Soil carbon stocks and carbon sequestration rates in seminatural grassland in Aso region, Kumamoto, Southern Japan.

PubMed

Toma, Yo; Clifton-Brown, John; Sugiyama, Shinji; Nakaboh, Makoto; Hatano, Ryusuke; Fernández, Fabián G; Ryan Stewart, J; Nishiwaki, Aya; Yamada, Toshihiko

2013-06-01

Global soil carbon (C) stocks account for approximately three times that found in the atmosphere. In the Aso mountain region of Southern Japan, seminatural grasslands have been maintained by annual harvests and/or burning for more than 1000 years. Quantification of soil C stocks and C sequestration rates in Aso mountain ecosystem is needed to make well-informed, land-use decisions to maximize C sinks while minimizing C emissions. Soil cores were collected from six sites within 200 km(2) (767-937 m asl.) from the surface down to the k-Ah layer established 7300 years ago by a volcanic eruption. The biological sources of the C stored in the Aso mountain ecosystem were investigated by combining C content at a number of sampling depths with age (using (14) C dating) and δ(13) C isotopic fractionation. Quantification of plant phytoliths at several depths was used to make basic reconstructions of past vegetation and was linked with C-sequestration rates. The mean total C stock of all six sites was 232 Mg C ha(-1) (28-417 Mg C ha(-1) ), which equates to a soil C sequestration rate of 32 kg C ha(-1)  yr(-1) over 7300 years. Mean soil C sequestration rates over 34, 50 and 100 years were estimated by an equation regressing soil C sequestration rate against soil C accumulation interval, which was modeled to be 618, 483 and 332 kg C ha(-1)  yr(-1) , respectively. Such data allows for a deeper understanding in how much C could be sequestered in Miscanthus grasslands at different time scales. In Aso, tribe Andropogoneae (especially Miscanthus and Schizoachyrium genera) and tribe Paniceae contributed between 64% and 100% of soil C based on δ(13) C abundance. We conclude that the seminatural, C4 -dominated grassland system serves as an important C sink, and worthy of future conservation. © 2013 Blackwell Publishing Ltd.

Eruption mechanism as inferred from geomagnetic changes with special attention to the 1989 1990 activity of Aso volcano

NASA Astrophysics Data System (ADS)

Tanaka, Yoshikazu

1993-06-01

Geomagnetic changes associated with the volcanic activity of Aso volcano were detected with a dense network of continuously recording proton-precession magnetometers during the period from June 1989 to June 1990. Magnetic date clearly indicate that changes in the magnetization within the volcano are most probably caused by temperature changes. This activity can be divided into five stages, which are characterized by magnetization and demagnetization of the volcano. These magnetic changes with durations of a few months are definitely correlated with some typical volcanic events at the crater as well as the volcanic tremor activity. The demagnetization stage appears when the vent is covered by a water pool or the volcanic activity is enhanced. The magnetization stage follows the opening of a vent and several large explosions which make the vent permeable. The source of magnetic changes lies at a depth of about 200 m below the crater rim in the southwestern part of the active crater. Magnetic moments responsible for observed magnetic changes at stages 1, 2 and 4 are 3.4, -5.2 and -2.2 Wbm, respectively. The corresponding source volume is estimated as a single sphere of radius 40-50 m or a spherical shell of 100 m or so. An effective mechanism of rapid heating/cooling exists, which is ascribed to the interaction of groundwater and superheated vapor, i.e., a shallow hydrothermal system. This hydrothermal system driven by the surface cap of the vent, controls every feature of the eruptions at the final outlet of Aso volcano.

Synthesis, crystal structure, thermal analysis and dielectric properties of Rb4(SO4)(HSO4)2(H3AsO4) compound

NASA Astrophysics Data System (ADS)

Belhaj Salah, M.; Nouiri, N.; Jaouadi, K.; Mhiri, T.; Zouari, N.

2018-01-01

A new inorganic Rb4(SO4)(HSO4)2(H3AsO4) compound was prepared. It was found to crystallize in the monoclinic system (P21 space group) with the following lattice parameters: a = 5868 (1) Å, b = 13,579(2) Å, c = 11,809 (3) Å and β = 94,737 (1)°. The structure is characterized by SO42-, HSO4- and H3AsO4 tetrahedra connected by hydrogen bridge to form two types of dimmer (H(8)S(2)O4- … S(1)O42- and H(12)S(2)O4- … H3AsO4). These dimmers are interconnected by both hydrogen bonds O(14)sbnd H(14)· · ·O(4) and O(15)sbnd H(15)· · ·O(2). They are also linked by the hydrogen bridge assured by the hydrogen atoms H(2), H(3) and H(4) of the H3AsO4 group to build the chain S(1)O4⋯H3AsO4 which are parallel to the ''a'',direction. The rubidium cations are coordinated by eight oxygen atoms with Rbsbnd O distance ranging from 2893(8) to 3.415(6) Å. The existence of Osbnd H and (S/As)sbnd O bonds in the structure at room temperature has been confirmed by IR and Raman spectroscopy in the frequency ranges 4000-400 cm-1and 1200 - 50 cm-1, respectively. Thermal analysis of Rb4(HSO4)(HSO4)2(H3AsO4) showed that the transformation to high temperature phase occurs at 407 K by one-step process. Thermal decomposition of the product takes place at much higher temperatures, with an onset of approximately 522 K. The first transition detected by differential scanning calorimetry (DSC) was also analyzed by dielectric and conductivity measurements using the impedance spectroscopy techniques. The conductivity in the high temperature phase at 428 K is 1.04 × 10-3 Ω-1 cm-1, and the activation energy for the proton transport is 0.36 eV. The conductivity relaxation parameters associated with the high disorder protonic conduction have been examined from analysis of the M"/M"max spectrum measured in a wide temperature range. Transport properties of this material appear to be due to the proton hopping mechanism. The obtained results show that this transition is protonic by nature.

Crystal structure of BaMn2(AsO4)2 containing discrete [Mn4O18]28- units.

PubMed

Alcantar, Salvador; Ledbetter, Hollis R; Ranmohotti, Kulugammana G S

2017-12-01

In our attempt to search for mixed alkaline-earth and transition metal arsenates, the title compound, barium dimanganese(II) bis-(arsenate), has been synthesized by employing a high-temperature RbCl flux. The crystal structure of BaMn 2 (AsO 4 ) 2 is made up of MnO 6 octa-hedra and AsO 4 tetra-hedra assembled by sharing corners and edges into infinite slabs with composition [Mn 2 (AsO 4 ) 2 ] 2- that extend parallel to the ab plane. The barium cations reside between parallel slabs maintaining the inter-slab connectivity through coordination to eight oxygen anions. The layered anionic framework comprises weakly inter-acting [Mn 4 O 18 ] 28- tetra-meric units. In each tetra-mer, the manganese(II) cations are in a planar arrangement related by a center of inversion. Within the slabs, the tetra-meric units are separated from each other by 6.614 (2) Å (Mn⋯Mn distances). The title compound has isostructural analogues amongst synthetic Sr M 2 ( X O 4 ) 2 compounds with M = Ni, Co, and X = As, P.

Topologically identical, but geometrically isomeric layers in hydrous α-, β-Rb[UO2(AsO3OH)(AsO2(OH)2)]·H2O and anhydrous Rb[UO2(AsO3OH)(AsO2(OH)2)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yu, Na; Klepov, Vladislav V.; Villa, Eric M.

The hydrothermal reaction of uranyl nitrate with rubidium nitrate and arsenic (III) oxide results in the formation of polymorphic α- and β-Rb[UO2(AsO3OH)(AsO2(OH)2)]·H2O (α-, β-RbUAs) and the anhydrous phase Rb[UO2(AsO3OH)(AsO2(OH)2)] (RbUAs). These phases were structurally, chemically and spectroscopically characterized. The structures of all three compounds are based upon topologically identical, but geometrically isomeric layers. The layers are linked with each other by means of the Rb cations and hydrogen bonding. Dehydration experiments demonstrate that water deintercalation from hydrous α- and β-RbUAs yields anhydrous RbUAs via topotactic reactions.

Monoclinic structure of hydroxylpyromorphite Pb10(PO4)6(OH)2 - hydroxylmimetite Pb10(AsO4)6(OH)2 solid solution series

NASA Astrophysics Data System (ADS)

Giera, Alicja; Manecki, Maciej; Borkiewicz, Olaf; Zelek, Sylwia; Rakovan, John; Bajda, Tomasz; Marchlewski, Tomasz

2016-04-01

Seven samples of hydroxyl analogues of pyromorphite-mimetite solid solutions series were synthesized from aqueous solutions at 80° C in a computer-controlled chemistate: 200 mL aqueous solutions of 0.05M Pb(NO3)2 and 0.03M KH2AsO4 and/or KH2PO4 were dosed with a 0.25 mL/min rate to a glass beaker, which initially contained 100 mL of distilled water. Constant pH of 8 was maintained using 2M KOH. The syntheses yielded homogeneous fine-grained white precipitates composition of which was close to theoretical Pb10[(PO4)6-x(AsO4)x](OH)2, where x = 0, 1, 2, 3, 4, 5, 6. High-resolution powder X-ray diffraction data were obtained in transmission geometry at the beamline 11-BM at the Advanced Photon Source (Argonne National Laboratory in Illinois, USA). The structure Rietveld refinements based on starting parameters of either hexagonal hydroxylpyromorphite or monoclinic mimetite-M were performed using GSAS+EXPGUI software. Apatite usually crystallizes in the hexagonal crystal system with the space group P63/m. For the first time, however, the lowering of the hexagonal to monoclinic crystal symmetry was observed in the hydroxyl variety of pyromorphite-mimetite solid solution series. This is indicated by better fitting of the modeled monoclinic structure to the experimental data. The same is not the case for analogous calcium hydroxylapatite series Ca5(PO4)3OH - Ca5(AsO4)3OH (Lee et al. 2009). Systematical linear increase of unit cell parameters is observed with As substitution from a=9.88, b=19.75, and c=7.43 for Pb10(PO4)6(OH)2 to a=10.23, b=20.32, and c=7.51 for Pb10(AsO4)6(OH)2. A strong pseudohexagonal character (γ ≈ 120° and b ≈ 2a) of the analyzed monoclinic phases was established. This work is partially funded by AGH research grant no 11.11.140.319 and partially by Polish NCN grant No 2011/01/M/ST10/06999. Lee Y.J., Stephens P.W., Tang Y., Li W., Philips B.L., Parise J.B., Reeder R.J., 2009. Arsenate substitution in hydroxylapatite: Structural characterization

Polarized-neutron investigation of magnetic ordering and spin dynamics in BaCo2(AsO4)2 frustrated honeycomb-lattice magnet.

PubMed

Regnault, L-P; Boullier, C; Lorenzo, J E

2018-01-01

The magnetic properties of the cobaltite BaCo 2 (AsO 4 ) 2 , a good realization of the quasi two-dimensional frustrated honeycomb-lattice system with strong planar anisotropy, have been reinvestigated by means of spherical neutron polarimetry with CRYOPAD. From accurate measurements of polarization matrices both on elastic and inelastic contributions as a function of the scattering vector Q , we have been able to determine the low-temperature magnetic structure of BaCo 2 (AsO 4 ) 2 and reveal its puzzling in-plane spin dynamics. Surprisingly, the ground-state structure (described by an incommensurate propagation vector [Formula: see text], with [Formula: see text] and [Formula: see text]) appears to be a quasi-collinear structure, and not a simple helix, as previously determined. In addition, our results have revealed the existence of a non-negligible out-of-plane moment component [Formula: see text]/Co 2+ , representing about 10% of the in-plane component, as demonstrated by the presence of finite off-diagonal elements [Formula: see text] and [Formula: see text] of the polarization matrix, both on elastic and inelastic magnetic contributions. Despite a clear evidence of the existence of a slightly inelastic contribution of structural origin superimposed to the magnetic excitations at the scattering vectors [Formula: see text] and [Formula: see text] (energy transfer [Formula: see text] meV), no strong inelastic nuclear-magnetic interference terms could be detected so far, meaning that the nuclear and magnetic degrees of freedom have very weak cross-correlations. The strong inelastic [Formula: see text] and [Formula: see text] matrix elements can be understood by assuming that the magnetic excitations in BaCo 2 (AsO 4 ) 2 are spin waves associated with trivial anisotropic precessions of the magnetic moments involved in the canted incommensurate structure.

Salt shell fallout during the ash eruption at the Nakadake crater, Aso volcano, Japan: evidence of an underground hydrothermal system surrounding the erupting vent

NASA Astrophysics Data System (ADS)

Shinohara, Hiroshi; Geshi, Nobuo; Yokoo, Akihiko; Ohkura, Takahiro; Terada, Akihiko

2018-03-01

A hot and acid crater lake is located in the Nakadake crater, Aso volcano, Japan. The volume of water in the lake decreases with increasing activity, drying out prior to the magmatic eruptions. Salt-rich materials of various shapes were observed, falling from the volcanic plume during the active periods. In May 2011, salt flakes fell from the gas plume emitted from an intense fumarole when the acid crater lake was almost dry. The chemical composition of these salt flakes was similar to those of the salts formed by the drying of the crater lake waters, suggesting that they originated from the crater lake water. The salt flakes are likely formed by the drying up of the crater lake water droplets sprayed into the plume by the fumarolic gas jet. In late 2014, the crater lake dried completely, followed by the magmatic eruptions with continuous ash eruptions and intermittent Strombolian explosions. Spherical hollow salt shells were observed on several occasions during and shortly after the weak ash eruptions. The chemical composition of the salt shells was similar to the salts formed by the drying of the crater lake water. The hollow structure of the shells suggests that they were formed by the heating of hydrothermal solution droplets suspended by a mixed stream of gas and ash in the plume. The salt shells suggest the existence of a hydrothermal system beneath the crater floor, even during the course of magmatic eruptions. Instability of the magmatic-hydrothermal interface can cause phreatomagmatic explosions, which often occur at the end of the eruptive phase of this volcano.

Using Himawari-8, estimation of SO2 cloud altitude at Aso volcano eruption, on October 8, 2016

NASA Astrophysics Data System (ADS)

Ishii, Kensuke; Hayashi, Yuta; Shimbori, Toshiki

2018-02-01

It is vital to detect volcanic plumes as soon as possible for volcanic hazard mitigation such as aviation safety and the life of residents. Himawari-8, the Japan Meteorological Agency's (JMA's) geostationary meteorological satellite, has high spatial resolution and sixteen observation bands including the 8.6 μm band to detect sulfur dioxide (SO2). Therefore, Ash RGB composite images (RED: brightness temperature (BT) difference between 12.4 and 10.4 μm, GREEN: BT difference between 10.4 and 8.6 μm, BLUE: 10.4 μm) discriminate SO2 clouds and volcanic ash clouds from meteorological clouds. Since the Himawari-8 has also high temporal resolution, the real-time monitoring of ash and SO2 clouds is of great use. A phreatomagmatic eruption of Aso volcano in Kyushu, Japan, occurred at 01:46 JST on October 8, 2016. For this eruption, the Ash RGB could detect SO2 cloud from Aso volcano immediately after the eruption and track it even 12 h after. In this case, the Ash RGB images every 2.5 min could clearly detect the SO2 cloud that conventional images such as infrared and split window could not detect sufficiently. Furthermore, we could estimate the height of the SO2 cloud by comparing the Ash RGB images and simulations of the JMA Global Atmospheric Transport Model with a variety of height parameters. As a result of comparison, the top and bottom height of the SO2 cloud emitted from the eruption was estimated as 7 and 13-14 km, respectively. Assuming the plume height was 13-14 km and eruption duration was 160-220 s (as estimated by seismic observation), the total emission mass of volcanic ash from the eruption was estimated as 6.1-11.8 × 108 kg, which is relatively consistent with 6.0-6.5 × 108 kg from field survey. [Figure not available: see fulltext.

Temporal Variation in Oscillatory Characteristics of Long-period Tremor at Aso Volcano, Japan.

NASA Astrophysics Data System (ADS)

Yamamoto, M.; Ohkura, T.; Kaneshima, S.; Kawakatsu, H.

2017-12-01

At Aso volcano, Japan, various kinds of volcanic signals with broad frequency contents have been observed since 1930s. One of these signals is long-period tremor (LPT) with a dominant period of around 15 s, which is intermittently emitted from the volcano regardless of the surface activity. Our broadband seismic observations have revealed that LPTs are a kind of resonance oscillation of a crack-like conduit beneath the crater. In this study, aiming to detect a temporal variation of volcanic system, we analyze the long-term variation of LPTs from 1994 to the present.We first examine the temporal variation of dominant periods of LPTs (fundamental mode of around 15 s and the first overtone of around 7 s) using the continuous data recorded at broadband stations close to the active crater. The result shows a clear temporal change in the dominant periods of LPTs in 2003-2005 and 2014-2015. In 2003-2005, the periods of the two modes show correlated temporal change, and it can be interpreted as compositional and/or thermal change of hydrothermal fluids. On the other hand, in 2014-2015, the period of first overtone is almost constant at around 8 s, while that of the fundamental mode shows relatively large temporal fluctuations between 16 s and 12 s. To explain the different behavior among the two resonant modes, we examine the oscillatory characteristics of a fluid-filled crack having linearly varying thickness. With this model, we find that the ratio between resonance periods becomes smaller than that in the case of a flat crack having constant thickness. This behavior can be understood by considering the effective thickness of the crack depends on the wavelength of each resonant mode. Based on these results, the different temporal variation of dominant periods can be interpreted by depth-dependent thickness of the crack-like conduit which may be caused by pressurization and/or intrusion of magma at deeper portion of the conduit. These results suggest the importance of

The oscillation model of hydrothermal dynamics beneath Aso volcano, southwest Japan after small eruption on May 2011: A new understanding model using repeated absolute and relative gravity measurement

NASA Astrophysics Data System (ADS)

Sofyan, Yayan; Nishijima, Jun; Fujimitsu, Yasuhiro; Yoshikawa, Shin; Kagiyama, Tsuneomi; Ohkura, Takahiro

2016-01-01

At the end of 2010, the seismic activity in Aso volcano intensely increased and water level in the Nakadake crater decreased until early in 2011, then was followed by a small eruption in May 2011. After the eruption and heavy rain, the volcanic activity subsided to calm period, crater bottom was refilled with water, and water level increased in the Nakadake crater. The next tremor reappeared in 2014 and tracked to eruption in November 2014. This eruptive pattern and water level variation in the crater repeatedly appeared on the surface, and it should be related to the hydrothermal dynamics beneath Aso volcano. We initiated the gravity measurements in relation to hydrothermal dynamics in the subsurface of Aso volcano using Scintrex CG-5 (549) and LaCoste Romberg type G-1016 relative gravimeter at 28 benchmarks in April 2011, one month before the eruption. The repeated gravity measurements continue to monitor Aso volcano with a series of the measurement after the eruption in every three months to a half year. We analyze the gravity variation from 2011 to 2014 between the time of the phreatic and strombolian eruption. The measurements covered the area more than 60 km2 in the west side of Aso caldera. A new gravity network was also installed in May 2010 at seven benchmarks using A10-017 absolute gravimeter, which re-occupied in October 2010, June 2011 and two benchmarks in June 2014. As a result, the gravity changes distinguish hydrothermal dynamic in the subsurface, which has a direct correlation to water level fluctuation in the crater, after the first eruption and before the second discharge. The monitoring data notice large gravity changes between the surveys at benchmarks around Nakadake crater and Kusasenri area. The simple 3D inversion models of the 4-D gravity data deduce the density contrast distribution beneath Aso volcano. The inversion and mass change result generate the oscillation typical as a new understanding model. The variation of the mass shows a

Analysis of gas jetting and fumarole acoustics at Aso Volcano, Japan

DOE PAGES

McKee, Kathleen; Fee, David; Yokoo, Akihiko; ...

2017-03-30

The gas-thrust region of a large volcanic eruption column is predominately a momentum-driven, fluid flow process that perturbs the atmosphere and produces sound akin to noise from jet and rocket engines, termed “jet noise”. In this paper, we aim to enhance understanding of large-scale volcanic jets by studying an accessible, less hazardous fumarolic jet. We characterize the acoustic signature of ~ 2.5-meter wide vigorously jetting fumarole at Aso Volcano, Japan using a 5-element infrasound array located on the nearby crater. The fumarole opened on 13 July 2015 on the southwest flank of the partially collapsed pyroclastic cone within Aso Volcano'smore » Naka-dake crater and had persistent gas jetting, which produced significant audible jet noise. The array was ~ 220 m from the fumarole and 57.6° from the vertical jet axis, a recording angle not typically feasible in volcanic environments. Array processing is performed to distinguish fumarolic jet noise from wind. Highly correlated periods are characterized by sustained, low-amplitude signal with a 7–10 Hz spectral peak. Finite difference time domain method numerical modeling suggests the influence of topography near the vent and along the propagation path significantly affects the spectral content, complicating comparisons with laboratory jet noise. The fumarolic jet has a low estimated Mach number (0.3 to 0.4) and measured temperature of ~ 260 °C. The Strouhal number for infrasound from volcanic jet flows and geysers is not known; thus we assume a peak Strouhal number of 0.19 based on pure-air laboratory jet experiments. This assumption leads to an estimated exit velocity of the fumarole of ~ 79 to 132 m/s. Finally, using published gas composition data from 2003 to 2009, the fumarolic vent area estimated from thermal infrared images, and estimated jet velocity, we estimate total volatile flux at ~ 160–270 kg/s (14,000–23,000 t/d).« less

Analysis of gas jetting and fumarole acoustics at Aso Volcano, Japan

DOE Office of Scientific and Technical Information (OSTI.GOV)

McKee, Kathleen; Fee, David; Yokoo, Akihiko

The gas-thrust region of a large volcanic eruption column is predominately a momentum-driven, fluid flow process that perturbs the atmosphere and produces sound akin to noise from jet and rocket engines, termed “jet noise”. In this paper, we aim to enhance understanding of large-scale volcanic jets by studying an accessible, less hazardous fumarolic jet. We characterize the acoustic signature of ~ 2.5-meter wide vigorously jetting fumarole at Aso Volcano, Japan using a 5-element infrasound array located on the nearby crater. The fumarole opened on 13 July 2015 on the southwest flank of the partially collapsed pyroclastic cone within Aso Volcano'smore » Naka-dake crater and had persistent gas jetting, which produced significant audible jet noise. The array was ~ 220 m from the fumarole and 57.6° from the vertical jet axis, a recording angle not typically feasible in volcanic environments. Array processing is performed to distinguish fumarolic jet noise from wind. Highly correlated periods are characterized by sustained, low-amplitude signal with a 7–10 Hz spectral peak. Finite difference time domain method numerical modeling suggests the influence of topography near the vent and along the propagation path significantly affects the spectral content, complicating comparisons with laboratory jet noise. The fumarolic jet has a low estimated Mach number (0.3 to 0.4) and measured temperature of ~ 260 °C. The Strouhal number for infrasound from volcanic jet flows and geysers is not known; thus we assume a peak Strouhal number of 0.19 based on pure-air laboratory jet experiments. This assumption leads to an estimated exit velocity of the fumarole of ~ 79 to 132 m/s. Finally, using published gas composition data from 2003 to 2009, the fumarolic vent area estimated from thermal infrared images, and estimated jet velocity, we estimate total volatile flux at ~ 160–270 kg/s (14,000–23,000 t/d).« less

The susceptibility analysis of landslides induced by earthquake in Aso volcanic area, Japan, scoping the prediction

NASA Astrophysics Data System (ADS)

Kubota, Tetsuya; Takeda, Tsuyoshi

2017-04-01

Kumamoto earthquake on April 16th 2016 in Kumamoto prefecture, Kyushu Island, Japan with intense seismic scale of M7.3 (maximum acceleration = 1316 gal in Aso volcanic region) yielded countless instances of landslide and debris flow that induced serious damages and causalities in the area, especially in the Aso volcanic mountain range. Hence, field investigation and numerical slope stability analysis were conducted to delve into the characteristics or the prediction factors of the landslides induced by this earthquake. For the numerical analysis, Finite Element Method (FEM) and CSSDP (Critical Slip Surface analysis by Dynamic Programming theory based on limit equilibrium method) were applied to the landslide slopes with seismic acceleration observed. These numerical analysis methods can automatically detect the landslide slip surface which has minimum Fs (factor of safety). The various results and the information obtained through this investigation and analysis were integrated to predict the landslide susceptible slopes in volcanic area induced by earthquakes and rainfalls of their aftermath, considering geologic-geomorphologic features, geo-technical characteristics of the landslides and vegetation effects on the slope stability. Based on the FEM or CSSDP results, the landslides occurred in this earthquake at the mild gradient slope on the ridge have the safety factor of slope Fs=2.20 approximately (without rainfall nor earthquake, and Fs>=1.0 corresponds to stable slope without landslide) and 1.78 2.10 (with the most severe rainfall in the past) while they have approximately Fs=0.40 with the seismic forces in this earthquake (horizontal direction 818 gal, vertical direction -320 gal respectively, observed in the earthquake). It insists that only in case of earthquakes the landslide in volcanic sediment apt to occur at the mild gradient slopes as well as on the ridges with convex cross section. Consequently, the following results are obtained. 1) At volcanic

Application of Sawdust modified with m-DMDHEU/Choline chloride for the removal of CrO4 2- and H2AsO4 - in water

NASA Astrophysics Data System (ADS)

Dang, N. T.; Quan, P. T.; Sang, P. H. P.

2017-06-01

This article studies the use of acacia auriculiformis wood sawdust modified with 4,5-dihydroxy-1,3-bis (methoxymethyl) imidazolidin-2-one (m-DMDHEU) and choline chloride for separating CrO4 2- and H2AsO4 - ions in water. NaOH 0.2N/ethanol 70° solution was used to remove lignin from the raw material, the material was then immersed in m-DMDHEU/choline chloride aqueous solution for 24 hours, after that the material was activated at 140oC for one hour. The ability to adsorb and exchange ions of the material was examined using solutions containing CrO4 2- and H2AsO4 - ions in different conditions. The results suggested that the ability to separate CrO4 2- and H2AsO4 - ions of the modified material was better than that of anion resin at pH = 7.0; the chromate adsorption capacity was the highest in acidic condition; the presence of arsenate (V) anions had no effect on the ability to remove chromate. Lastly, the modified material was used to treat water samples containing concentrations of arsenic similar to groundwater in several arsenic-contaminated areas of Vietnam.

The thermal signature of Aso Volcano during unrest episodes detected from space and ground-based measurements

NASA Astrophysics Data System (ADS)

Cigolini, Corrado; Coppola, Diego; Yokoo, Akihiko; Laiolo, Marco

2018-04-01

The thermal signature of Aso Volcano (Nakadake) during unrest episodes has been analyzed by combining the MODIS-MIROVA data set (2000-2017) with high-resolution images (LANDSAT 8 OLI and Sentinel 2) and ground-based thermal observations (2013-2017). The site of major activity (crater 1) is located at the summit of the volcano and is composed by a fumarole field (located in the South Area) and an acidic lake (replaced by a Central Pit during Strombolian phases). The volcanic radiative power (VRP) obtained by nighttime satellite data during the reference period was mainly below 3 MW. This thermal threshold marks the transition from high fumarole activity (HFA) to Strombolian eruptions (SE). However, periods characterized by sporadic phreatic eruptions (PE, eventually bearing phreatomagmatic episodes), which is the prevalent phase during unrest episodes, exhibit very low VRP values, being around 0.5 MW, or below. The statistical analysis of satellite data shows that the transition from HFA to Strombolian activity (which started on August 2014 and ceased in May 2015) occurs when VRP values are above the cited 3 MW threshold. In particular during marked Strombolian phases (November-December 2014), the radiative power was higher than 4 MW, reaching peak values up to 15.6 MW (on December 7, 2014, i.e., 10 days after the major Strombolian explosion of November 27). Conversely, ground-based measurements show that heat fluxes recorded by FLIR T440 Thermo-camera on the fumarole field of the South Area has been relatively stable around 2 MW until February 2015. Their apparent temperatures were fluctuating around 490-575 °C before the major Strombolian explosive event, whereas those recorded at the active vent, named Central Pit, reached their maxima slightly above 600 °C; then both exhibited a decreasing trend in the following days. During the Strombolian activity, the crater lake dried out and was then replenished by early July, 2016. Then, volcanic activity shifted back to

Ionic molal conductivities, activity coefficients, and dissociation constants of HAsO42− and H2AsO4− from 5 to 90°C and ionic strengths from 0.001 up to 3 mol kg−1 and applications in natural systems

USGS Publications Warehouse

Zhu, Xiangyu; Nordstrom, D. Kirk; McCleskey, R. Blaine; Wang, Rucheng

2016-01-01

Arsenic is known to be one of the most toxic inorganic elements, causing worldwide environmental contamination. However, many fundamental properties related to aqueous arsenic species are not well known which will inhibit our ability to understand the geochemical behavior of arsenic (e.g. speciation, transport, and solubility). Here, the electrical conductivity of Na2HAsO4 solutions has been measured over the concentration range of 0.001–1 mol kg−1 and the temperature range of 5–90°C. Ionic strength and temperature-dependent equations were derived for the molal conductivity of HAsO42−and H2AsO4− aqueous ions. Combined with speciation calculations and the approach used by McCleskey et al. (2012b), these equations can be used to calculate the electrical conductivities of arsenic-rich waters having a large range of effective ionic strengths (0.001–3 mol kg−1) and temperatures (5–90°C). Individual ion activity coefficients for HAsO42− and H2AsO4− in the form of the Hückel equation were also derived using the mean salt method and the mean activity coefficients of K2HAsO4 (0.001–1 mol kg−1) and KH2AsO4 (0.001–1.3 mol kg−1). A check on these activity coefficients was made by calculating mean activity coefficients for Na2HAsO4 and NaH2AsO4 solutions and comparing them to measured values. At the same time Na-arsenate complexes were evaluated. The NaH2AsO40 ion pair is negligible in NaH2AsO4 solutions up to 1.3 mol kg−1. The NaHAsO4− ion pair is important in NaHAsO4 solutions >0.1 mol kg−1 and the formation constant of 100.69 was confirmed. The enthalpy, entropy, free energy and heat capacity for the second and third arsenic acid dissociation reactions were calculated from pH measurements. These properties have been incorporated into a widely used geochemical calculation code WATEQ4F and applied to natural arsenic waters. For arsenic spiked water samples from Yellowstone National Park, the mean difference between the

Mineral and chemical variations within an ash-flow sheet from Aso caldera, Southwestern Japan

USGS Publications Warehouse

Lipman, P.W.

1967-01-01

Although products of individual volcanic eruptions, especially voluminous ash-flow eruptions, have been considered among the best available samples of natural magmas, detailed petrographic and chemical study indicates that bulk compositions of unaltered Pleistocene ash-flow tuffs from Aso caldera, Japan, deviate significantly from original magmatic compositions. The last major ash-flow sheet from Aso caldera is as much as 150 meters thick and shows a general vertical compositional change from phenocryst-poor rhyodacite upward into phenocryst-rich trachyandesite; this change apparently reflects in inverse order a compositionally zoned magma chamber in which more silicic magma overlay more mafic magma. Details of these magmatic variations were obscured, however, by: (1) mixing of compositionally distinct batches of magma during upwelling in the vent, as indicated by layering and other heterogeneities within single pumice lumps; (2) mixing of particulate fragments-pumice lumps, ash, and phenocrysts-of varied compositions during emplacement, with the result that separate pumice lenses from a single small outcrop may have a compositional range nearly as great as the bulk-rook variation of the entire sheet; (3) density sorting of phenocrysts and ash during eruption and emplacement, resulting in systematic modal variations with distance from the caldera; (4) addition of xenocrysts, resulting in significant contamination and modification of proportions of crystals in the tuffs; and (5) ground-water leaching of glassy fractions during hydration after cooling. Similar complexities characterize ash-flow tuffs under study in southwestern Nevada and in the San Juan Mountains, Colorado, and probably are widespread in other ash-flow fields as well. Caution and careful planning are required in study of the magmatic chemistry and phenocryst mineralogy of these rocks. ?? 1967 Springer-Verlag.

Coordination of Advanced Solar Observatory (ASO) Science Working Group (SWG) for the study of instrument accommodation and operational requirements on space station

NASA Technical Reports Server (NTRS)

Wu, S. T.

1989-01-01

The objectives are to coordinate the activities of the Science Working Group (SWG) of the Advanced Solar Observatory (ASO) for the study of instruments accommodation and operation requirements on board space station. In order to facilitate the progress of the objective, two conferences were organized, together with two small group discussions.

Structural Development and Oxidation of the Takanoobane Rhyolite Lava in Aso Caldera, Japan

NASA Astrophysics Data System (ADS)

Furukawa, K.; Uno, K.; Miyagi, I.

2007-12-01

The Takanoobane rhyolite lava (hereafter described as the TR lava) is distributed in the western part of Aso caldera, middle Kyushu Island, SW Japan. The TR lava is one of the central cones. The volume, SiO2 contents and K-Ar age are 0.14km3 (Miyabuchi et al., 2004), 71-72% (Furukawa, 2006) and 51+-5ka (Matsumoto et al., 1991), respectively. The TR lava was effused in a subaerial environment. In this study, we show vertical structural variation and the development of the TR lava from the four drilling cores obtained by Aso Volcanological Laboratory in 2001-2002. The TR lava is about 90m thick in the proximal part, and the internal structures are divided into three parts: Alternation of the pumiceous layers and the obsidian layers (the upper part), the crystalline rhyolite layer (the central part), and the obsidian layer (the lower part). This structural variation apparently resembles to that of the Obsidian Dome near long valley caldera in eastern California (Manley and Fink, 1987). The central crystalline rhyolite layer of the TR lava is characterized by the development of the flow structure, which is composed of interconnected minute cavities. The shapes and sizes of the structure are varied from stubby or lens to flattened and from a few mm to above 5 cm in length, respectively. The morphology of the flow structure tends to be flattened with distance from the source region. It is probably due to shear stress caused by the lava movement We described the vertical variation of the mineral assemblage of Fe-Ti oxides. It shows that the highly oxidized Fe-Ti oxides tend to be distributed around the flow structure. Thus, the part is selectively oxidized. It is supported also by the rock magnetic experiments. Above studies and cooling history calculated by a numerical modeling show that the oxidation was caused by the increasing of fO2 at the part. We interpret that the increasing of fO2 was caused by the release of hydrogen from the degassing lava. Hydrogen should be

Kamarizaite, Fe{3/3+}(AsO4)2(OH)3 · 3H2O, a new mineral species, arsenate analogue of tinticite

NASA Astrophysics Data System (ADS)

Chukanov, N. V.; Pekov, I. V.; Möckel, S.; Mukhanova, A. A.; Belakovsky, D. I.; Levitskaya, L. A.; Bekenova, G. K.

2010-12-01

Kamarizaite, a new mineral species, has been identified in the dump of the Kamariza Mine, Lavrion mining district, Attica Region, Greece, in association with goethite, scorodite, and jarosite. It was named after type locality. Kamarizaite occurs as fine-grained monomineralic aggregates (up to 3 cm across) composed of platy crystals up to 1 μm in size and submicron kidney-shaped segregations. The new mineral is yellow to beige, with light yellow streak. The Mohs hardness is about 3. No cleavage is observed. The density measured by hydrostatic weighing is 3.16(1) g/cm3, and the calculated density is 3.12 g/cm3. The wavenumbers of absorption bands in the IR spectrum of kamarizaite are (cm-1; s is strong band, w is weak band): 3552, 3315s, 3115, 1650w, 1620w, 1089, 911s, 888s, 870, 835s, 808s, 614w, 540, 500, 478, 429. According to TG and IR data, complete dehydration and dehydroxylation in vacuum (with a weight loss of 15.3(1)%) occurs in the temperature range 110-420°C. Mössbauer data indicate that all iron in kamarizaite is octahedrally coordinated Fe3+. Kamarizaite is optically biaxial, positive: n min = 1.825, n max = 1.835, n mean = 1.83(1) (for a fine-grained aggregate). The chemical composition of kamarizaite (electron microprobe, average of four point analyses) is as follows, wt %: 0.35 CaO, 41.78 Fe2O3, 39.89 As2O5, 1.49 SO3, 15.3 H2O (from TG data); the total is 98.81. The empirical formula calculated on the basis of (AsO4,SO4)2 is Ca0.03Fe{2.86/3+} (AsO4)1.90(SO4)0.10(OH)2.74 · 3.27H2O. The idealized formula is Fe{3/3+}(AsO4)2(OH)3 · 3H2O. Kamarizaite is an arsenate analogue of orthorhombic tinticite, space group Pccm, Pcc2, Pcmm, Pcm21, or Pc2 m; a = 21.32(1), b = 13.666(6), c =15.80(1) Å, V= 4603.29(5) Å3, Z= 16. The strongest reflections of the X-ray powder diffraction pattern [ bar d , Å ( I, %) ( hkl)] are: 6.61 (37) (112, 120), 5.85 (52) (311), 3.947 (100) (004, 032, 511), 3.396 (37) (133, 431), 3.332 (60) (314), 3.085 (58) (621, 414, 324

From drones to ASO: Using 'Structure-From-Motion' photogrammetry to quantify variations in snow depth at multiple scales

NASA Astrophysics Data System (ADS)

Skiles, M.

2017-12-01

The ability to accurately measure and manage the natural snow water reservoir in mountainous regions has its challenges, namely mapping of snowpack depth and snow water equivalent (SWE). Presented here is a scalable method that differentially maps snow depth using Structure from Motion (SfM); a photogrammetric technique that uses 2d images to create a 3D model/Digital Surface Model (DSM). There are challenges with applying SfM to snow, namely, relatively uniform snow brightness can make it difficult to produce quality images needed for processing, and vegetation can limit the ability to `see' through the canopy to map both the ground and snow beneath. New techniques implemented in the method to adapt to these challenges will be demonstrated. Results include a time series at (1) the plot scale, imaged with an unmanned areal vehicle (DJI Phantom 2 adapted with Sony A5100) over the Utah Department of Transportation Atwater Study Plot in Little Cottonwood Canyon, UT, and at (2) the mountain watershed scale, imaged from the RGB camera aboard the Airborne Snow Observatory (ASO), over the headwaters of the Uncompahgre River in the San Juan Mountains, CO. At the plot scale we present comparisons to measured snow depth, and at the watershed scale we present comparisons to the ASO lidar DSM. This method is of interest due to its low cost relative to lidar, making it an accessible tool for snow research and the management of water resources. With advancing unmanned aerial vehicle technology there are implications for scalability to map snow depth, and SWE, across large basins.

A Connection from Arctic Stratospheric Ozone to El Niño-Southern Oscillation

NASA Astrophysics Data System (ADS)

Xie, F.

2017-12-01

Antarctic stratospheric ozone depletion is thought to influence the Southern Hemisphere tropospheric climate. Recently, Arctic stratospheric ozone (ASO) variations have been found to affect the middle-high latitude tropospheric climate in the Northern Hemisphere. This paper demonstrates that the impact of ASO can extend to the tropics, with the ASO variations leading El Niño-Southern Oscillation (ENSO) events by about 20 months. Using observations, analysis, and simulations, the connection between ASO and ENSO is established by combining the high-latitude stratosphere to troposphere pathway with the extratropical to tropical climate teleconnection. This shows that the ASO radiative anomalies influence the North Pacific Oscillation (NPO), and the anomalous NPO and induced Victoria Mode anomalies link to the North Pacific circulation that then influences ENSO. Our results imply that incorporating realistic and time-varying ASO into climate system models may help to improve ENSO predictions.

Behavioral and endocrine changes following antisense oligonucleotide-induced reduction in the rat NOP receptor.

PubMed

Blakley, Gregory G; Pohorecky, Larissa A; Benjamin, Daniel

2004-02-01

Compared with the use of classic receptor ligands, antisense oligonucleotides (ASO) targeted at specific central nervous system receptors are an effective alternative in experiments designed to examine the behavioral role of such systems. The nociception/orphaninFQ (N/OFQ) system has been implicated in mediating endocrine function, feeding, stress, pain, anxiety, and the rewarding effects of drugs of abuse. The objective of the current study was to examine whether long-term ASO-induced downregulation of N/OFQ's receptor (NOP) produced changes in endocrine, anxiety, nociception and ethanol's (EtOH's) locomotor activating properties. Male Long Evans rats were implanted with osmotic mini-pumps containing ASO for the NOP receptor. ASO was chronically infused for 26 days and, during this time, multiple behavioral and physiological measurements were conducted. ASO infusion significantly reduced expression of the NOP receptor in brain, confirmed by significant reductions of OFQ-stimulated [(35)S]-GTPgammaS binding in the paraventricular nucleus, prefrontal cortex, and septum. Behavioral changes were observed in ASO-treated animals including higher body temperature, increased water intake, decreased corticosterone (CORT) levels, decreased grooming in the open field, increased tail-flick latency, shorter durations on the open arms of the elevated plus maze, and heightened locomotor activity following EtOH. These behavioral, physiological and endocrine changes are relatively consistent with previous findings with agonists and antagonists for the NOP receptor and, taken together, suggest that ASO-induced downregulation of the NOP receptor is an effective method for studying the N/OFQ system.

Infrared and Raman spectroscopic characterizations on new Fe sulphoarsenate hilarionite (Fe2(III)(SO4)(AsO4)(OH)·6H2O): Implications for arsenic mineralogy in supergene environment of mine area

NASA Astrophysics Data System (ADS)

Liu, Jing; He, LiLe; Dong, Faqin; Frost, Ray L.

2017-01-01

Hilarionite (Fe2 (SO4)(AsO4)(OH)·6H2O) is a new Fe sulphoarsenates mineral, which recently is found in the famous Lavrion ore district, Atliki Prefecture, Greece. The spectroscopic study of hilarionite enriches the data of arsenic mineralogy in supergene environment of a mine area. The infrared and Raman means are used to characterize the molecular structure of this mineral. The IR bands at 875 and 905 cm- 1 are assigned to the antisymmetric stretching vibrations of AsO43 -. The IR bands at 1021, 1086 and 1136 cm- 1 correspond to the possible antisymmetric and symmetric stretching vibrations of SO42 -. The Raman bands at 807, 843 and 875 cm- 1 clearly show that arsenate components in the mineral structure, which are assigned to the symmetric stretching vibrations (ν1) of AsO43 - (807 and 843 cm- 1) and the antisymmetric vibration (ν3) (875 cm- 1). IR bands provide more sulfate information than Raman, which can be used as the basis to distinguish hilarionite from kaňkite. The powder XRD data shows that hilarionite has obvious differences with the mineral structure of kaňkite. The thermoanalysis and SEM-EDX results show that hilarionite has more sulfate than arsenate.

The 3D Mesonet Concept: Extending Networked Surface Meteorological Tower Observations Through Unmanned Aircraft Systems

NASA Astrophysics Data System (ADS)

Chilson, P. B.; Fiebrich, C. A.; Huck, R.; Grimsley, J.; Salazar-Cerreno, J.; Carson, K.; Jacob, J.

2017-12-01

Fixed monitoring sites, such as those in the US National Weather Service Automated Surface Observing System (ASOS) and the Oklahoma Mesonet provide valuable, high temporal resolution information about the atmosphere to forecasters and the general public. The Oklahoma Mesonet is comprised of a network of 120 surface sites providing a wide array of atmospheric measurements up to a height of 10 m with an update time of five minutes. The deployment of small unmanned aircraft to collect in-situ vertical measurements of the atmospheric state in conjunction with surface conditions has potential to significantly expand weather observation capabilities. This concept can enhance the safety of individuals and support commerce through improved observations and short-term forecasts of the weather and other environmental variables in the lower atmosphere. We report on a concept of adding the capability of collecting vertical atmospheric measurements (profiles) through the use of unmanned aerial systems (UAS) at remote Oklahoma sites deemed suitable for this application. While there are a number of other technologies currently available that can provide measurements of one or a few variables, the proposed UAS concept will be expandable and modular to accommodate several different sensor packages and provide accurate in-situ measurements in virtually all weather conditions. Such a system would facilitate off-site maintenance and calibration and would provide the ability to add new sensors as they are developed or as new requirements are identified. The small UAS must be capable of accommodating the weight of all sensor packages and have lighting, communication, and aircraft avoidance systems necessary to meet existing or future FAA regulations. The system must be able to operate unattended, which necessitates the inclusion of risk mitigation measures such as a detect and avoid radar and the ability to transmit and receive transponder signals. Moreover, the system should be able to

High-Elevation Evapotranspiration Estimates During Drought: Using Streamflow and NASA Airborne Snow Observatory SWE Observations to Close the Upper Tuolumne River Basin Water Balance

NASA Astrophysics Data System (ADS)

Henn, Brian; Painter, Thomas H.; Bormann, Kat J.; McGurk, Bruce; Flint, Alan L.; Flint, Lorraine E.; White, Vince; Lundquist, Jessica D.

2018-02-01

Hydrologic variables such as evapotranspiration (ET) and soil water storage are difficult to observe across spatial scales in complex terrain. Streamflow and lidar-derived snow observations provide information about distributed hydrologic processes such as snowmelt, infiltration, and storage. We use a distributed streamflow data set across eight basins in the upper Tuolumne River region of Yosemite National Park in the Sierra Nevada mountain range, and the NASA Airborne Snow Observatory (ASO) lidar-derived snow data set over 3 years (2013-2015) during a prolonged drought in California, to estimate basin-scale water balance components. We compare snowmelt and cumulative precipitation over periods from the ASO flight to the end of the water year against cumulative streamflow observations. The basin water balance residual term (snow melt plus precipitation minus streamflow) is calculated for each basin and year. Using soil moisture observations and hydrologic model simulations, we show that the residual term represents short-term changes in basin water storage over the snowmelt season, but that over the period from peak snow water equivalent (SWE) to the end of summer, it represents cumulative basin-mean ET. Warm-season ET estimated from this approach is 168 (85-252 at 95% confidence), 162 (0-326) and 191 (48-334) mm averaged across the basins in 2013, 2014, and 2015, respectively. These values are lower than previous full-year and point ET estimates in the Sierra Nevada, potentially reflecting reduced ET during drought, the effects of spatial variability, and the part-year time period. Using streamflow and ASO snow observations, we quantify spatially-distributed hydrologic processes otherwise difficult to observe.

Global Space Weather Observational Network: Challenges and China's Contribution

NASA Astrophysics Data System (ADS)

Wang, C.

2017-12-01

To understand space weather physical processes and predict space weather accurately, global space-borne and ground-based space weather observational network, making simultaneous observations from the Sun to geo-space (magnetosphere, ionosphere and atmosphere), plays an essential role. In this talk, we will present the advances of the Chinese space weather science missions, including the ASO-S (Advanced Space-borne Solar Observatory), MIT (Magnetosphere - Ionosphere- Thermosphere Coupling Exploration), and the ESA-China joint space weather science mission SMILE (Solar wind - Magnetosphere - Ionosphere Link Explore), a new mission to image the magnetosphere. Compared to satellites, ground-based monitors are cheap, convenient, and provide continuous real-time data. We will also introduce the Chinese Meridian Project (CMP), a ground-based program fully utilizing the geographic location of the Chinese landmass to monitor the geo-space environment. CMP is just one arm of a larger program that Chinese scientists are proposing to the international community. The International Meridian Circle Program (IMCP) for space weather hopes to connect chains of ground-based monitors at the longitudinal meridians 120 deg E and 60 deg W. IMCP takes advantage of the fact that these meridians already have the most monitors of any on Earth, with monitors in Russia, Australia, Brazil, the United States, Canada, and other countries. This data will greatly enhance the ability of scientists to monitor and predict the space weather worldwide.

Hilarionite, Fe{2/3+}(SO4)(AsO4)(OH) · 6H2O, a new supergene mineral from Lavrion, Greece

NASA Astrophysics Data System (ADS)

Pekov, I. V.; Chukanov, N. V.; Yapaskurt, V. O.; Rusakov, V. S.; Belakovsky, D. I.; Turchkova, A. G.; Voudouris, P.; Magganas, A.; Katerinopoulos, A.

2014-12-01

A new mineral, hilarionite, ideally Fe{2/3+} (SO4)(AsO4)(OH) · 6H2O, has been found in the Hilarion Mine, Agios Konstantinos, Kamariza, Lavrion district, Attiki Prefecture, Greece. It was formed in the oxidation zone of a sulfide-rich orebody in association with goethite, gypsum, bukovskyite, jarosite, melanterite, chalcanthite, allophane, and azurite. Hilarionite occurs as light green (typically with an olive or grayish tint) to light yellowish green spherulites (up to 1 mm in size) and bunches of prismatic to acicular "individuals" up to 0.5 mm long that are in fact near-parallel or divergent aggregates of very thin, curved fibers up to 0.3 mm long and usually lesser than 2 μm thick. The luster is silky to vitreous. The Mohs' hardness is ca. 2. Hilarionite is ductile, its "individuals" are flexible and inelastic; fracture is uneven or splintery. D(meas) = 2.40(5), D(calc) = 2.486 g/cm3. IR spectrum shows the presence of arsenate and sulfate groups and H2O molecules in significant amounts. The Mössbauer spectrum indicates the presence of Fe3+ at two six-fold coordinated sites and the absence of Fe2+. Hilarionite is optically biaxial (+), α = 1.575(2), γ = 1.64(2), 2 V is large. The chemical composition (electron microprobe, average of 7 point analyses; H2O determined by modified Penfield method) is as follows, wt %: 0.03 MnO, 0.18 CuO, 0.17 ZnO, 33.83 Fe2O3, 0.22 P2O5, 18.92 As2O5, 22.19 SO3, 26.3 H2O, total is 101.82%. The empirical formula calculated on the basis of 15 O is: (Fe{1.90/3+}Cu0.01Zn0.01)Σ1.92[(SO4)1.24(AsO4)0.74(PO4)0.01]Σ1.99(OH)1.01 · 6.03H2O. The X-ray powder diffraction data show close structural relationship of hilarionite and kaňkite, Fe{2/3+}(AsO4)2 · 7H2O. Hilarionite is monoclinic, space group C2/ m, Cm or C2, a = 18.53(4), b = 17.43(3), c = 7.56(1) Å, β = 94.06(15)°, V = 2436(3) Å3, Z = 8. The strongest reflections in the X-ray powder diffraction pattern ( d, Å- I[ hkl]) are: 12.66-100[110], , 5.00-10[22l], , 4

Targeted delivery of antisense oligonucleotides to hepatocytes using triantennary N-acetyl galactosamine improves potency 10-fold in mice.

PubMed

Prakash, Thazha P; Graham, Mark J; Yu, Jinghua; Carty, Rick; Low, Audrey; Chappell, Alfred; Schmidt, Karsten; Zhao, Chenguang; Aghajan, Mariam; Murray, Heather F; Riney, Stan; Booten, Sheri L; Murray, Susan F; Gaus, Hans; Crosby, Jeff; Lima, Walt F; Guo, Shuling; Monia, Brett P; Swayze, Eric E; Seth, Punit P

2014-07-01

Triantennary N-acetyl galactosamine (GalNAc, GN3: ), a high-affinity ligand for the hepatocyte-specific asialoglycoprotein receptor (ASGPR), enhances the potency of second-generation gapmer antisense oligonucleotides (ASOs) 6-10-fold in mouse liver. When combined with next-generation ASO designs comprised of short S-cEt (S-2'-O-Et-2',4'-bridged nucleic acid) gapmer ASOs, ∼ 60-fold enhancement in potency relative to the parent MOE (2'-O-methoxyethyl RNA) ASO was observed. GN3: -conjugated ASOs showed high affinity for mouse ASGPR, which results in enhanced ASO delivery to hepatocytes versus non-parenchymal cells. After internalization into cells, the GN3: -ASO conjugate is metabolized to liberate the parent ASO in the liver. No metabolism of the GN3: -ASO conjugate was detected in plasma suggesting that GN3: acts as a hepatocyte targeting prodrug that is detached from the ASO by metabolism after internalization into the liver. GalNAc conjugation also enhanced potency and duration of the effect of two ASOs targeting human apolipoprotein C-III and human transthyretin (TTR) in transgenic mice. The unconjugated ASOs are currently in late stage clinical trials for the treatment of familial chylomicronemia and TTR-mediated polyneuropathy. The ability to translate these observations in humans offers the potential to improve therapeutic index, reduce cost of therapy and support a monthly dosing schedule for therapeutic suppression of gene expression in the liver using ASOs. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

Pharmacology of a Central Nervous System Delivered 2′-O-Methoxyethyl–Modified Survival of Motor Neuron Splicing Oligonucleotide in Mice and Nonhuman Primates

PubMed Central

Chun, Seung J.; Norris, Daniel A.; Hung, Gene; Lee, Sam; Matson, John; Fey, Robert A.; Gaus, Hans; Hua, Yimin; Grundy, John S.; Krainer, Adrian R.; Henry, Scott P.; Bennett, C. Frank

2014-01-01

Spinal muscular atrophy (SMA) is a debilitating neuromuscular disease caused by the loss of survival of motor neuron (SMN) protein. Previously, we demonstrated that ISIS 396443, an antisense oligonucleotide (ASO) targeted to the SMN2 pre-mRNA, is a potent inducer of SMN2 exon 7 inclusion and SMN protein expression, and improves function and survival of mild and severe SMA mouse models. Here, we demonstrate that ISIS 396443 is the most potent ASO in central nervous system (CNS) tissues of adult mice, compared with several other chemically modified ASOs. We evaluated methods of ISIS 396443 delivery to the CNS and characterized its pharmacokinetics and pharmacodynamics in rodents and nonhuman primates (NHPs). Intracerebroventricular bolus injection is a more efficient method of delivering ISIS 396443 to the CNS of rodents, compared with i.c.v. infusion. For both methods of delivery, the duration of ISIS 396443–mediated SMN2 splicing correction is long lasting, with maximal effects still observed 6 months after treatment discontinuation. Administration of ISIS 396443 to the CNS of NHPs by a single intrathecal bolus injection results in widespread distribution throughout the spinal cord. Based upon these preclinical studies, we have advanced ISIS 396443 into clinical development. PMID:24784568

Cellular uptake mediated by epidermal growth factor receptor facilitates the intracellular activity of phosphorothioate-modified antisense oligonucleotides

PubMed Central

Wang, Shiyu; Allen, Nickolas; Vickers, Timothy A; Revenko, Alexey S; Sun, Hong; Liang, Xue-hai; Crooke, Stanley T

2018-01-01

Abstract Chemically modified antisense oligonucleotides (ASOs) with phosphorothioate (PS) linkages have been extensively studied as research and therapeutic agents. PS-ASOs can enter the cell and trigger cleavage of complementary RNA by RNase H1 even in the absence of transfection reagent. A number of cell surface proteins have been identified that bind PS-ASOs and mediate their cellular uptake; however, the mechanisms that lead to productive internalization of PS-ASOs are not well understood. Here, we characterized the interaction between PS-ASOs and epidermal growth factor receptor (EGFR). We found that PS-ASOs trafficked together with EGF and EGFR into clathrin-coated pit structures. Their co-localization was also observed at early endosomes and inside enlarged late endosomes. Reduction of EGFR decreased PS-ASO activity without affecting EGF-mediated signaling pathways and overexpression of EGFR increased PS-ASO activity in cells. Furthermore, reduction of EGFR delays PS-ASO trafficking from early to late endosomes. Thus, EGFR binds to PS-ASOs at the cell surface and mediates essential steps for active (productive) cellular uptake of PS-ASOs through its cargo-dependent trafficking processes which migrate PS-ASOs from early to late endosomes. This EGFR-mediated process can also serve as an additional model to better understand the mechanism of intracellular uptake and endosomal release of PS-ASOs. PMID:29514240

Broadband Ground Motion Observation and Simulation for the 2016 Kumamoto Earthquake

NASA Astrophysics Data System (ADS)

Miyake, H.; Chimoto, K.; Yamanaka, H.; Tsuno, S.; Korenaga, M.; Yamada, N.; Matsushima, T.; Miyakawa, K.

2016-12-01

During the 2016 Kumamoto earthquake, strong motion data were widely recorded by the permanent dense triggered strong motion network of K-NET/KiK-net and seismic intensity meters installed by local government and JMA. Seismic intensities close to the MMI 9-10 are recorded twice at the Mashiki town, and once at the Nishihara village and KiK-net Mashiki (KMMH16 ground surface). Near-fault records indicate extreme ground motion exceeding 400 cm/s in 5% pSv at a period of 1 s for the Mashiki town and 3-4 s for the Nishihara village. Fault parallel velocity components are larger between the Mashiki town and the Nishihara village, on the other hand, fault normal velocity components are larger inside the caldera of the Aso volcano. The former indicates rupture passed through along-strike stations, and the latter stations located at the forward rupture direction (e.g., Miyatake, 1999). In addition to the permanent observation, temporary continuous strong motion stations were installed just after the earthquake in the Kumamoto city, Mashiki town, Nishihara village, Minami-Aso village, and Aso town, (e.g., Chimoto et al., 2016; Tsuno et al., 2016; Yamanaka et al. 2016). This study performs to estimate strong motion generation areas for the 2016 Kumamoto earthquake sequence using the empirical Green's function method, then to simulate broadband ground motions for both the permanent and temporary strong motion stations. Currently the target period range is between 0.1 s to 5-10 s due to the signal-to-noise ratio of element earthquakes used for the empirical Green's functions. We also care fault dimension parameters N within 4 to 10 to avoid spectral sags and artificial periodicity. The simulated seismic intensities as well as fault normal and parallel velocity components will be discussed.

Blood Pressure Lowering and Safety Improvements With Liver Angiotensinogen Inhibition in Models of Hypertension and Kidney Injury.

PubMed

Mullick, Adam E; Yeh, Steve T; Graham, Mark J; Engelhardt, Jeffery A; Prakash, Thazha P; Crooke, Rosanne M

2017-09-01

Uncontrolled hypertension is an important contributor to cardiovascular disease. Despite the armamentarium of antihypertensive treatments, there remains a need for novel agents effective in individuals who cannot reach acceptable blood pressure levels. Inhibitors targeting the renin-angiotensin-aldosterone system (RAAS) are widely used but may not optimally inhibit RAAS and demonstrate an acceptable safety profile. Experiments were conducted to characterize a series of AGT (angiotensinogen) antisense oligonucleotides (ASOs) and compare their efficacy and tolerability to traditional RAAS blockade. AGT ASOs which target multiple systemic sites of AGT versus an N-acetylgalactosamine-conjugated AGT ASO that targets the liver were compared with captopril and losartan. Spontaneously hypertensive rats fed an 8% NaCl diet, a model of malignant hypertension resistant to standard RAAS inhibitors, demonstrated robust and durable blood pressure reductions with AGT ASO treatments, which was not observed with standard RAAS blockade. Studies in rat models of acute kidney injury produced by salt deprivation revealed kidney injury with ASO treatment that reduced kidney-expressed AGT, but not in animals treated with the N-acetylgalactosamine AGT ASO despite comparable plasma AGT reductions. Administration of either captopril or losartan also produced acute kidney injury during salt deprivation. Thus, intrarenal RAAS derived from kidney AGT, and inhibited by the standard of care, contributes to the maintenance of renal function during severe RAAS challenge. Such improvements in efficacy and tolerability by a liver-selective AGT inhibitor could be desirable in individuals not at their blood pressure goal with existing RAAS blockade. © 2017 American Heart Association, Inc.

Spleen-specific suppression of TNF-alpha by cationic hydrogel-delivered antisense nucleotides for the prevention of arthritis in animal models.

PubMed

Dong, Lei; Xia, Suhua; Chen, Huan; Chen, Jiangning; Zhang, Junfeng

2009-09-01

This study developed a transplantable platform based on cationic hydrogels to deliver antisense oligodeoxynucleotides (ASOs) targeting the mRNA of TNF-alpha. Cationic agarose (c-agarose) was obtained by conjugating ethylenediamine to agarose via an N,N'-carbonyldiimidazole (CDI)-activation method. ASO-c-agarose system was constructed by mixing ASO in cationic agarose gel of proper concentration and gelation temperature. In vivo assessment of ASO distribution suggested that the system specifically target to spleen, wherein the c-agarose-delivered ASO had a concentration remarkably 50-fold higher than that of the naked ASO. The distribution of c-agarose-delivered ASO was scarcely detectable in liver and kidney. Next, three types of animal models were setup to evaluate the therapeutic efficacies of ASO-Gel, including the adjuvant-induced arthritis (AA), carrageen/lipopolysaccharide (LPS)-induced arthritis (CLA) and collagen-induced arthritis (CIA) models. The effects of ASO-c-agarose in alleviating inflammation and tissue destruction were evidenced in more than 90% of the testing animals, with decrease of main inflammatory cytokines, lightening of joint swelling and tissue damage, as well as increase in their body weights. All these findings suggest that this highly operable devise for the conveyance of antisense nucleotides together with its spleen-targeting property, could become a useful means of antisense-based therapeutics against rheumatoid arthritis and other diseases.

Spin and charge controlled by antisymmetric spin-orbit coupling in a triangular-triple-quantum-dot Kondo system

NASA Astrophysics Data System (ADS)

Koga, M.; Matsumoto, M.; Kusunose, H.

2018-05-01

We study a local antisymmetric spin-orbit (ASO) coupling effect on a triangular-triple-quantum-dot (TTQD) system as a theoretical proposal for a new application of the Kondo physics to nanoscale devices. The electric polarization induced by the Kondo effect is strongly correlated with the spin configurations and molecular orbital degrees of freedom in the TTQD. In particular, an abrupt sign reversal of the emergent electric polarization is associated with a quantum critical point in a magnetic field, which can also be controlled by the ASO coupling that changes the mixing weight of different orbital components in the TTQD ground state.

47 CFR 87.525 - Scope of service.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Automatic Weather Stations (AWOS/ASOS) § 87.525 Scope of service. Automatic weather observation stations (AWOS) and automatic surface observation stations (ASOS) must provide up-to-date weather information including the time of the latest weather sequence, altimeter setting, wind speed and direction, dew point...

47 CFR 87.525 - Scope of service.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Automatic Weather Stations (AWOS/ASOS) § 87.525 Scope of service. Automatic weather observation stations (AWOS) and automatic surface observation stations (ASOS) must provide up-to-date weather information including the time of the latest weather sequence, altimeter setting, wind speed and direction, dew point...

47 CFR 87.525 - Scope of service.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Automatic Weather Stations (AWOS/ASOS) § 87.525 Scope of service. Automatic weather observation stations (AWOS) and automatic surface observation stations (ASOS) must provide up-to-date weather information including the time of the latest weather sequence, altimeter setting, wind speed and direction, dew point...

47 CFR 87.525 - Scope of service.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Automatic Weather Stations (AWOS/ASOS) § 87.525 Scope of service. Automatic weather observation stations (AWOS) and automatic surface observation stations (ASOS) must provide up-to-date weather information including the time of the latest weather sequence, altimeter setting, wind speed and direction, dew point...

47 CFR 87.525 - Scope of service.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Automatic Weather Stations (AWOS/ASOS) § 87.525 Scope of service. Automatic weather observation stations (AWOS) and automatic surface observation stations (ASOS) must provide up-to-date weather information including the time of the latest weather sequence, altimeter setting, wind speed and direction, dew point...

Antisense oligonucleotide therapy rescues disruptions in organization of exploratory movements associated with Usher syndrome type 1C in mice.

PubMed

Donaldson, Tia N; Jennings, Kelsey T; Cherep, Lucia A; McNeela, Adam M; Depreux, Frederic F; Jodelka, Francine M; Hastings, Michelle L; Wallace, Douglas G

2018-02-15

Usher syndrome, Type 1C (USH1C) is an autosomal recessive inherited disorder in which a mutation in the gene encoding harmonin is associated with multi-sensory deficits (i.e., auditory, vestibular, and visual). USH1C (Usher) mice, engineered with a human USH1C mutation, exhibit these multi-sensory deficits by circling behavior and lack of response to sound. Administration of an antisense oligonucleotide (ASO) therapeutic that corrects expression of the mutated USH1C gene, has been shown to increase harmonin levels, reduce circling behavior, and improve vestibular and auditory function. The current study evaluates the organization of exploratory movements to assess spatial organization in Usher mice and determine the efficacy of ASO therapy in attenuating any such deficits. Usher and heterozygous mice received the therapeutic ASO, ASO-29, or a control, non-specific ASO treatment at postnatal day five. Organization of exploratory movements was assessed under dark and light conditions at two and six-months of age. Disruptions in exploratory movement organization observed in control-treated Usher mice were consistent with impaired use of self-movement and environmental cues. In general, ASO-29 treatment rescued organization of exploratory movements at two and six-month testing points. These observations are consistent with ASO-29 rescuing processing of multiple sources of information and demonstrate the potential of ASO therapies to ameliorate topographical disorientation associated with other genetic disorders. Copyright © 2017 Elsevier B.V. All rights reserved.

Utility of High Temporal Resolution Observations for Heat Health Event Characterization

NASA Astrophysics Data System (ADS)

Palecki, M. A.

2017-12-01

Many heat health watch systems produce a binary on/off warning when conditions are predicted to exceed a given threshold during a day. Days with warnings and their mortality/morbidity statistics are analyzed relative to days not warned to determine the impacts of the event on human health, the effectiveness of warnings, and other statistics. The climate analyses of the heat waves or extreme temperature events are often performed with hourly or daily observations of air temperature, humidity, and other measured or derived variables, especially the maxima and minima of these data. However, since the beginning of the century, 5-minute observations are readily available for many weather and climate stations in the United States. NOAA National Centers for Environmental Information (NCEI) has been collecting 5-minute observations from the NOAA Automated Surface Observing System (ASOS) stations since 2000, and from the U.S. Climate Reference Network (USCRN) stations since 2005. This presentation will demonstrate the efficacy of utilizing 5-minute environmental observations to characterize heat waves by counting the length of time conditions exceed extreme thresholds based on individual and multiple variables and on derived variables such as the heat index. The length and depth of recovery periods between daytime heating periods will also be examined. The length of time under extreme conditions will influence health outcomes for those directly exposed. Longer periods of dangerous conditions also could increase the chances for poor health outcomes for those only exposed intermittently through cumulative impacts.

Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol

PubMed Central

Nishina, Tomoko; Numata, Junna; Nishina, Kazutaka; Yoshida-Tanaka, Kie; Nitta, Keiko; Piao, Wenying; Iwata, Rintaro; Ito, Shingo; Kuwahara, Hiroya; Wada, Takeshi; Mizusawa, Hidehiro; Yokota, Takanori

2015-01-01

We developed an efficient system for delivering short interfering RNA (siRNA) to the liver by using α-tocopherol conjugation. The α-tocopherol–conjugated siRNA was effective and safe for RNA interference–mediated gene silencing in vivo. In contrast, when the 13-mer LNA (locked nucleic acid)-DNA gapmer antisense oligonucleotide (ASO) was directly conjugated with α-tocopherol it showed markedly reduced silencing activity in mouse liver. Here, therefore, we tried to extend the 5′-end of the ASO sequence by using 5′-α-tocopherol–conjugated 4- to 7-mers of unlocked nucleic acid (UNA) as a “second wing.” Intravenous injection of mice with this α-tocopherol–conjugated chimeric ASO achieved more potent silencing than ASO alone in the liver, suggesting increased delivery of the ASO to the liver. Within the cells, the UNA wing was cleaved or degraded and α-tocopherol was released from the 13-mer gapmer ASO, resulting in activation of the gapmer. The α-tocopherol–conjugated chimeric ASO showed high efficacy, with hepatic tropism, and was effective and safe for gene silencing in vivo. We have thus identified a new, effective LNA-DNA gapmer structure in which drug delivery system (DDS) molecules are bound to ASO with UNA sequences. PMID:25584900

15 CFR 946.5 - Change in operations-commissioning and decommissioning.

Code of Federal Regulations, 2013 CFR

2013-01-01

... NATIONAL WEATHER SERVICE MODERNIZATION OF THE NATIONAL WEATHER SERVICE § 946.5 Change in operations—commissioning and decommissioning. (a) Before commissioning any new NEXRAD or ASOS weather observation system...; technical coordination with weather service users has been completed; and the system satisfactorily supports...

15 CFR 946.5 - Change in operations-commissioning and decommissioning.

Code of Federal Regulations, 2011 CFR

2011-01-01

... NATIONAL WEATHER SERVICE MODERNIZATION OF THE NATIONAL WEATHER SERVICE § 946.5 Change in operations—commissioning and decommissioning. (a) Before commissioning any new NEXRAD or ASOS weather observation system...; technical coordination with weather service users has been completed; and the system satisfactorily supports...

15 CFR 946.5 - Change in operations-commissioning and decommissioning.

Code of Federal Regulations, 2012 CFR

2012-01-01

... NATIONAL WEATHER SERVICE MODERNIZATION OF THE NATIONAL WEATHER SERVICE § 946.5 Change in operations—commissioning and decommissioning. (a) Before commissioning any new NEXRAD or ASOS weather observation system...; technical coordination with weather service users has been completed; and the system satisfactorily supports...

15 CFR 946.5 - Change in operations-commissioning and decommissioning.

Code of Federal Regulations, 2014 CFR

2014-01-01

... NATIONAL WEATHER SERVICE MODERNIZATION OF THE NATIONAL WEATHER SERVICE § 946.5 Change in operations—commissioning and decommissioning. (a) Before commissioning any new NEXRAD or ASOS weather observation system...; technical coordination with weather service users has been completed; and the system satisfactorily supports...

15 CFR 946.5 - Change in operations-commissioning and decommissioning.

Code of Federal Regulations, 2010 CFR

2010-01-01

... NATIONAL WEATHER SERVICE MODERNIZATION OF THE NATIONAL WEATHER SERVICE § 946.5 Change in operations—commissioning and decommissioning. (a) Before commissioning any new NEXRAD or ASOS weather observation system...; technical coordination with weather service users has been completed; and the system satisfactorily supports...

Premature Extinction of the Weather Observer: How Much Risk is the Air Force Assuming

DTIC Science & Technology

2015-12-01

is impacted in some way by the weather and the forecast. DOD assets are exposed to haz- ardous weather conditions each year, the effects of which...discussion of ASOS accuracy follows and is accompanied by an assessment of sur- face weather observations’ impacts to operations as a function of time as well...from some back-up techniques. This section details current knowledge of AMOS and cor- responding impacts of AMOS employment. Current Fielded Systems

Implementation of an Automatic Stop Order and Initial Antibiotic Exposure in Very Low Birth Weight Infants.

PubMed

Tolia, Veeral N; Desai, Sujata; Qin, Huanying; Rayburn, Polli D; Poon, Grace; Murthy, Karna; Ellsbury, Dan L; Chiruvolu, Arpitha

2017-01-01

Objective  To evaluate if an antibiotic automatic stop order (ASO) changed early antibiotic exposure (use in the first 7 days of life) or clinical outcomes in very low birth weight (VLBW) infants. Study Design  We compared birth characteristics, early antibiotic exposure, morbidity, and mortality data in VLBW infants (with birth weight <= 1500 g) born 2 years before (pre-ASO group, n  = 313) to infants born in the 2 years after (post-ASO, n  = 361) implementation of an ASO guideline. Early antibiotic exposure was quantified by days of therapy (DOT) and antibiotic use > 48 hours. Secondary outcomes included mortality, early mortality, early onset sepsis (EOS), and necrotizing enterocolitis. Results  Birth characteristics were similar between the two groups. We observed reduced median antibiotic exposure (pre-ASO: 6.5 DOT vs. Post-ASO: 4 DOT; p  < 0.001), and a lower percentage of infants with antibiotic use > 48 hours (63.4 vs. 41.3%; p  < 0.001). There were no differences in mortality (12.1 vs 10.2%; p  = 0.44), early mortality, or other reported morbidities. EOS accounted for less than 10% of early antibiotic use. Conclusion  Early antibiotic exposure was reduced after the implementation of an ASO without changes in observed outcomes. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Earth observation taken by the Expedition 33 crew.

NASA Image and Video Library

2012-11-18

ISS033-E-022852 (18 Nov. 2012) --- This view, photographed by an Expedition 33 crew member on the International Space Station, highlights the 24-kilometer wide Aso caldera on the Japanese Island of Kyushu, formed during four explosive eruptions that took place from 300,000 to 90,000 years ago, according to scientists. These major eruptions produced pyroclastic flows and airfall tephra that covered much of Kyushu. As the eruptions emptied the magma chambers beneath the ancient volcanoes, they collapsed ? forming the caldera. Shadows highlight the caldera rim at left, while green vegetation covers slopes between the rim and caldera floor at right. Volcanic activity continued in the caldera following its formation, represented by 17 younger volcanoes including Naka-dake at center. Naka-dake is one of Japan?s most active volcanoes, with ash plumes produced from the summit crater as recently as June 2011. Another prominent crater, Kusasenri, is visible to the west of Naka-dake. This crater is the site of the Aso Volcano Museum as well as pasture for cows and horses. The Aso caldera floor is largely occupied by urban and agricultural land uses that present a gray to white speckled appearance in the image. Fields and cities surround the younger volcanic structures in the caldera center to the north, west, and south. Tan to yellow-brown regions along the crater rim, and along the lower slopes of the younger volcanic highlands in the central caldera, are lacking the dense tree cover indicated by greener areas in the image.

The use of LIDAR Technology for Measuring Mixing Heights under the Photochemical Assessment Monitoring Program; leveraging research under the joint DISCOVER-AQ/FRAPPÉ Missions

EPA Science Inventory

The operational use of ceilometers across the United States has been limited to detection of cloud-base heights across the Automatic Surface Observing Systems (ASOS) primarily operated by the National Weather Service and the Federal Aviation Administration. Continued improvements...

Peroxide-mediated desulfurization of phosphorothioate oligonucleotides and its prevention.

PubMed

Krotz, Achim H; Mehta, Rahul C; Hardee, Gregory E

2005-02-01

Desulfurization at the internucleotide phosphorothioate linkage of antisense oligonucleotides (ASOs) in dermatological formulations has been investigated using strong ion exchange chromatography and mass spectroscopy. The formation of phosphate diester linkages appeared to arise from a reaction between the phosphorothioate oligonucleotide and a potent oxidizing agent. Screening of excipients used in the formulation indicated that the cause of desulfurization was related to the presence of polyethylene glycol-derived nonionic surfactants MYRJ 52 or BRIJ 58. Autoxidation of the polyethylene glycol chain is suggested as the probable origin for the observed incompatibility. The ability of various antioxidants to prevent oxidative degradation of ASO-1 in simple test systems and in oil-in-water emulsions is described. It is found that in test systems both lipophilic and hydrophilic antioxidants are effective. However, in cream formulation (oil-in-water emulsions) of ASO-1 the addition of hydrophilic antioxidants L-cysteine or DL-alpha-lipoic acid has been shown to be superior in protecting the oligonucleotide from desulfurization upon storage. Copyright 2004 Wiley-Liss, Inc.

A preparation zone for volcanic explosions beneath Naka-dake crater, Aso volcano, as inferred from magnetotelluric surveys

NASA Astrophysics Data System (ADS)

Kanda, Wataru; Tanaka, Yoshikazu; Utsugi, Mitsuru; Takakura, Shinichi; Hashimoto, Takeshi; Inoue, Hiroyuki

2008-11-01

The 1st crater of Naka-dake, Aso volcano, is one of the most active craters in Japan, and known to have a characteristic cycle of activity that consists of the formation of a crater lake, drying-up of the lake water, and finally a Strombolian-type eruption. Recent observations indicate an increase in eruptive activity including a decrease in the level of the lake water, mud eruptions, and red hot glows on the crater wall. Temporal variations in the geomagnetic field observed around the craters of Naka-dake also indicate that thermal demagnetization of the subsurface rocks has been occurring in shallow subsurface areas around the 1st crater. Volcanic explosions act to release the energy transferred from magma or volcanic fluids. Measurement of the subsurface electrical resistivity is a promising method in investigating the shallow structure of the volcanic edifices, where energy from various sources accumulates, and in investigating the behaviors of magma and volcanic fluids. We carried out audio-frequency magnetotelluric surveys around the craters of Naka-dake in 2004 and 2005 to determine the detailed electrical structure down to a depth of around 1 km. The main objective of this study is to identify the specific subsurface structure that acts to store energy as a preparation zone for volcanic eruption. Two-dimensional inversions were applied to four profiles across the craters, revealing a strongly conductive zone at several hundred meters depth beneath the 1st crater and surrounding area. In contrast, we found no such remarkable conductor at shallow depths beneath the 4th crater, which has been inactive for 70 years, finding instead a relatively resistive body. The distribution of the rotational invariant of the magnetotelluric impedance tensor is consistent with the inversion results. This unusual shallow structure probably reflects the existence of a supply path of high-temperature volcanic gases to the crater bottom. We propose that the upper part of the

Growth Oscillatory Zoning in Erythrite, Ideally Co3(AsO4)2·8H2O: Structural Variations in Vivianite-Group Minerals

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antao, Sytle M.; Dhaliwal, Inayat

The crystal structure of an oscillatory zoned erythrite sample from Aghbar mine, Bou Azzer, Morocco, was refined using synchrotron high-resolution powder X-ray diffraction (HRPXRD) data, Rietveld refinement, space group C2/m, and Z = 2. The crystal contains two sets of oscillatory zones that appear to have developed during epitaxial growth. The unit-cell parameters obtained are a = 10.24799(3) Å, b = 13.42490(7) Å, c = 4.755885(8) Å, β = 105.1116(3)°, and V = 631.680(4) Å3. The empirical formula for erythrite, obtained with electron-probe micro-analysis (EPMA), is [Co2.78Zn0.11Ni0.07Fe0.04]Σ3.00(AsO4)2·8H2O. Erythrite belongs to the vivianite-type structure that contains M1O2(H2O)4 octahedra and M22O6(H2O)4 octahedralmore » dimers that are linked by TO4 (T5+ = As or P) tetrahedra to form complex layers parallel to the (010) plane. These layers are connected by hydrogen bonds. The average [6] = 2.122(1) Å and average [6] = 2.088(1) Å. With space group C2/m, there are two solid solutions: M3(AsO4)2·8H2O and M3(PO4)2·8H2O where M2+ = Mg, Fe, Co, Ni, or Zn. In these As- and P-series, using data from this study and from the literature, we find that their structural parameters evolve linearly with V and in a nearly parallel manner despite of the large difference in size between P5+ (0.170 Å) and As5+ (0.355 Å) cations. Average [4], [6], and [6] distances increase linearly with V. The average distance is affected by M atoms, whereas the average distance is unaffected because it contains shorter and stronger P–O bonds. Although As- and P-series occur naturally, there is no structural reason why similar V-series vivianite-group minerals do not occur naturally or cannot be synthesized.« less

Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish.

PubMed

Pauli, Andrea; Montague, Tessa G; Lennox, Kim A; Behlke, Mark A; Schier, Alexander F

2015-01-01

Antisense oligonucleotides (ASOs) are synthetic, single-strand RNA-DNA hybrids that induce catalytic degradation of complementary cellular RNAs via RNase H. ASOs are widely used as gene knockdown reagents in tissue culture and in Xenopus and mouse model systems. To test their effectiveness in zebrafish, we targeted 20 developmental genes and compared the morphological changes with mutant and morpholino (MO)-induced phenotypes. ASO-mediated transcript knockdown reproduced the published loss-of-function phenotypes for oep, chordin, dnd, ctnnb2, bmp7a, alk8, smad2 and smad5 in a dosage-sensitive manner. ASOs knocked down both maternal and zygotic transcripts, as well as the long noncoding RNA (lncRNA) MALAT1. ASOs were only effective within a narrow concentration range and were toxic at higher concentrations. Despite this drawback, quantitation of knockdown efficiency and the ability to degrade lncRNAs make ASOs a useful knockdown reagent in zebrafish.

System/observer/controller identification toolbox

NASA Technical Reports Server (NTRS)

Juang, Jer-Nan; Horta, Lucas G.; Phan, Minh

1992-01-01

System Identification is the process of constructing a mathematical model from input and output data for a system under testing, and characterizing the system uncertainties and measurement noises. The mathematical model structure can take various forms depending upon the intended use. The SYSTEM/OBSERVER/CONTROLLER IDENTIFICATION TOOLBOX (SOCIT) is a collection of functions, written in MATLAB language and expressed in M-files, that implements a variety of modern system identification techniques. For an open loop system, the central features of the SOCIT are functions for identification of a system model and its corresponding forward and backward observers directly from input and output data. The system and observers are represented by a discrete model. The identified model and observers may be used for controller design of linear systems as well as identification of modal parameters such as dampings, frequencies, and mode shapes. For a closed-loop system, an observer and its corresponding controller gain directly from input and output data.

Targeting of Repeated Sequences Unique to a Gene Results in Significant Increases in Antisense Oligonucleotide Potency

PubMed Central

Vickers, Timothy A.; Freier, Susan M.; Bui, Huynh-Hoa; Watt, Andrew; Crooke, Stanley T.

2014-01-01

A new strategy for identifying potent RNase H-dependent antisense oligonucleotides (ASOs) is presented. Our analysis of the human transcriptome revealed that a significant proportion of genes contain unique repeated sequences of 16 or more nucleotides in length. Activities of ASOs targeting these repeated sites in several representative genes were compared to those of ASOs targeting unique single sites in the same transcript. Antisense activity at repeated sites was also evaluated in a highly controlled minigene system. Targeting both native and minigene repeat sites resulted in significant increases in potency as compared to targeting of non-repeated sites. The increased potency at these sites is a result of increased frequency of ASO/RNA interactions which, in turn, increases the probability of a productive interaction between the ASO/RNA heteroduplex and human RNase H1 in the cell. These results suggest a new, highly efficient strategy for rapid identification of highly potent ASOs. PMID:25334092

The Group on Earth Observations and the Global Earth Observation System of Systems

NASA Astrophysics Data System (ADS)

Achache, J.

2006-05-01

The Group on Earth Observations (GEO) is leading a worldwide effort to build a Global Earth Observation System of Systems (GEOSS) over the next 10 years. The GEOSS vision, articulated in its 10-Year Implementation Plan, represents the consolidation of a global scientific and political consensus: the assessment of the state of the Earth requires continuous and coordinated observation of our planet at all scales. GEOSS aims to achieve comprehensive, coordinated and sustained observations of the Earth system in order to improve monitoring of the state of the Earth; increase understanding of Earth processes; and enhance prediction of the behaviour of the Earth system. After the World Summit on Sustainable Development in 2002 highlighted the urgent need for coordinated observations relating to the state of the Earth, GEO was established at the Third Earth Observation Summit in February 2005 and the GEOSS 10-Year Implementation Plan was endorsed. GEO currently involves 60 countries; the European Commission; and 43 international organizations and has begun implementation of the GEOSS 10-Year Implementation Plan. GEO programme activities cover nine societal benefit areas (Disasters; Health; Energy; Climate; Water; Weather; Ecosystems; Agriculture; Biodiversity) and five transverse or crosscutting elements (User Engagement; Architecture; Data Management; Capacity Building; Outreach). All these activities have as their final goal the establishment of the "system of systems" which will yield a broad range of basic societal benefits, including the reduction of loss of life and property from tsunamis, hurricanes, and other natural disasters; improved water resource and energy management; and improved understanding of environmental factors significant to public health. As a "system of systems", GEOSS will work with and build upon existing national, regional, and international systems to provide comprehensive, coordinated Earth observations from thousands of instruments worldwide

The macrophage as a Trojan horse for antisense oligonucleotide delivery.

PubMed

Novak, James S; Jaiswal, Jyoti K; Partridge, Terence A

2018-06-04

The gateway to the promised land of gene therapy has been obstructed by the problem of accurate and efficient delivery of therapeutic agents to their target sites. This is true both of constructs designed to directly express proteins of interest, and of constructs or agents aimed at modifying the expression of endogenous genes. It is recognized as a major impediment to the effective application of genetic therapies currently or incipiently in clinical trial. Our recent study has examined the mechanism underlying delivery of therapeutic antisense oligonucleotides (ASO) for treating the devastating muscle disease Duchenne muscular dystrophy [1]. Working to understand the mode of ASO delivery in DMD, we discovered that inflammatory cells act as a depot that locally stores the intravenously administered ASO. This local depot of ASO then becomes available to the muscle fibres by way of satellite cells that deliver their cargo by fusion with damaged fibres during muscle repair. This finding points to a potentially novel strategy for systemic ASO delivery, involving the use of the inflammatory cell as a Trojan horse. Such an approach would have the benefit not only of enhancing tissue-specific delivery of ASO, but also of reducing the impact of their rapid clearance from the circulation. Here, we discuss the issues surrounding ASO-mediated exon skipping efficacy for DMD, and outline research aimed at improving targeted ASO delivery.

Landslides triggered by an earthquake and heavy rainfalls at Aso volcano, Japan, detected by UAS and SfM-MVS photogrammetry

NASA Astrophysics Data System (ADS)

Saito, Hitoshi; Uchiyama, Shoichiro; Hayakawa, Yuichi S.; Obanawa, Hiroyuki

2018-12-01

Unmanned aerial systems (UASs) and structure-from-motion multi-view stereo (SfM-MVS) photogrammetry have attracted a tremendous amount of interest for use in the creation of high-definition topographic data for geoscientific studies. By using these techniques, this study examined the topographic characteristics of coseismic landslides triggered by the 2016 Kumamoto earthquake (Mw 7.1) in the Sensuikyo area (1.0 km2) at Aso volcano, Japan. The study area has frequently experienced rainfall-induced landslide events, such as those in 1990, 2001, and 2012. We obtained orthorectified images and digital surface models (DSMs) with a spatial resolution of 0.06 m before and after the 2016 Kumamoto earthquake. By using these high-definition images and DSMs, we detected a total of 54 coseismic landslides with volumes of 9.1-3994.6 m3. These landslides, many of which initiated near topographic ridges, were typically located on upside hillslopes of previous rainfall-induced landslide scars that formed in 2012. This result suggests that the topographic effect on seismic waves, i.e., amplification of ground acceleration, was important for coseismic landslide initiation in the study area. The average depth of the coseismic landslides was 1.5 m, which is deeper than the depth of the rainfall-induced landslides prior to these. The total sediment production of the coseismic landslides reached 2.5 × 104 m3/km2, which is of the same order as the sediment production triggered by the previous single heavy rainfall event. This result indicates that the effects of the 2016 Kumamoto earthquake in terms of sediment production and topographic changes were similar to those of the rainfall-induced landslide event in the study area.

Identification and characterization of intracellular proteins that bind oligonucleotides with phosphorothioate linkages

PubMed Central

Liang, Xue-hai; Sun, Hong; Shen, Wen; Crooke, Stanley T.

2015-01-01

Although the RNase H-dependent mechanism of inhibition of gene expression by chemically modified antisense oligonucleotides (ASOs) has been well characterized, little is known about the interactions between ASOs and intracellular proteins that may alter cellular localization and/or potency of ASOs. Here, we report the identification of 56 intracellular ASO-binding proteins using multi-step affinity selection approaches. Many of the tested proteins had no significant effect on ASO activity; however, some proteins, including La/SSB, NPM1, ANXA2, VARS and PC4, appeared to enhance ASO activities, likely through mechanisms related to subcellular distribution. VARS and ANXA2 co-localized with ASOs in endocytic organelles, and reduction in the level of VARS altered lysosome/ASO localization patterns, implying that these proteins may facilitate ASO release from the endocytic pathway. Depletion of La and NPM1 reduced nuclear ASO levels, suggesting potential roles in ASO nuclear accumulation. On the other hand, Ku70 and Ku80 proteins inhibited ASO activity, most likely by competition with RNase H1 for ASO/RNA duplex binding. Our results demonstrate that phosphorothioate-modified ASOs bind a set of cellular proteins that affect ASO activity via different mechanisms. PMID:25712094

Scientific and Regulatory Policy Committee Points-to-consider Paper*: Drug-induced Vascular Injury Associated with Nonsmall Molecule Therapeutics in Preclinical Development: Part 2. Antisense Oligonucleotides.

PubMed

Engelhardt, Jeffery A; Fant, Pierluigi; Guionaud, Silvia; Henry, Scott P; Leach, Michael W; Louden, Calvert; Scicchitano, Marshall S; Weaver, James L; Zabka, Tanja S; Frazier, Kendall S

2015-10-01

Drug-induced vascular injury (DIVI) is a recurrent challenge in the development of novel pharmaceutical agents. In recent years, DIVI has been occasionally observed in nonhuman primates given RNA-targeting therapeutics such as antisense oligonucleotide therapies (ASOs) during chronic toxicity studies. While DIVI in laboratory animal species has been well characterized for vasoactive small molecules, and immune-mediated responses against large molecule biotherapeutics have been well described, there is little published information regarding DIVI induced by ASOs to date. Preclinical DIVI findings in monkeys have caused considerable delays in development of promising new ASO therapies, because of the uncertainty about whether DIVI in preclinical studies is predictive of effects in humans, and the lack of robust biomarkers of DIVI. This review of DIVI discusses clinical and microscopic features of vasculitis in monkeys, their pathogenic mechanisms, and points to consider for the toxicologist and pathologist when confronted with ASO-related DIVI. Relevant examples of regulatory feedback are included to provide insight into risk assessment of ASO therapies. © 2015 by The Author(s).

Hepatotoxicity of high affinity gapmer antisense oligonucleotides is mediated by RNase H1 dependent promiscuous reduction of very long pre-mRNA transcripts

PubMed Central

Burel, Sebastien A.; Hart, Christopher E.; Cauntay, Patrick; Hsiao, Jill; Machemer, Todd; Katz, Melanie; Watt, Andy; Bui, Huynh-hoa; Younis, Husam; Sabripour, Mahyar; Freier, Susan M.; Hung, Gene; Dan, Amy; Prakash, T.P.; Seth, Punit P.; Swayze, Eric E.; Bennett, C. Frank; Crooke, Stanley T.; Henry, Scott P.

2016-01-01

High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, transcriptional profiles were collected from the livers of mice treated with a panel of highly efficacious hepatotoxic or non-hepatotoxic LNA ASOs. We observed highly selective transcript knockdown in mice treated with non-hepatotoxic LNA ASOs, while the levels of many unintended transcripts were reduced in mice treated with hepatotoxic LNA ASOs. This transcriptional signature was concurrent with on-target RNA reduction and preceded transaminitis. Remarkably, the mRNA transcripts commonly reduced by toxic LNA ASOs were generally not strongly associated with any particular biological process, cellular component or functional group. However, they tended to have much longer pre-mRNA transcripts. We also demonstrate that the off-target RNA knockdown and hepatotoxicity is attenuated by RNase H1 knockdown, and that this effect can be generalized to high affinity modifications beyond LNA. This suggests that for a certain set of ASOs containing high affinity modifications such as LNA, hepatotoxicity can occur as a result of unintended off-target RNase H1 dependent RNA degradation. PMID:26553810

The Airborne Snow Observatory: fusion of imaging spectrometer and scanning lidar for studies of mountain snow cover (Invited)

NASA Astrophysics Data System (ADS)

Painter, T. H.; Andreadis, K.; Berisford, D. F.; Goodale, C. E.; Hart, A. F.; Heneghan, C.; Deems, J. S.; Gehrke, F.; Marks, D. G.; Mattmann, C. A.; McGurk, B. J.; Ramirez, P.; Seidel, F. C.; Skiles, M.; Trangsrud, A.; Winstral, A. H.; Kirchner, P.; Zimdars, P. A.; Yaghoobi, R.; Boustani, M.; Khudikyan, S.; Richardson, M.; Atwater, R.; Horn, J.; Goods, D.; Verma, R.; Boardman, J. W.

2013-12-01

Snow cover and its melt dominate regional climate and water resources in many of the world's mountainous regions. However, we face significant water resource challenges due to the intersection of increasing demand from population growth and changes in runoff total and timing due to climate change. Moreover, increasing temperatures in desert systems will increase dust loading to mountain snow cover, thus reducing the snow cover albedo and accelerating snowmelt runoff. The two most critical properties for understanding snowmelt runoff and timing are the spatial and temporal distributions of snow water equivalent (SWE) and snow albedo. Despite their importance in controlling volume and timing of runoff, snowpack albedo and SWE are still poorly quantified in the US and not at all in most of the globe, leaving runoff models poorly constrained. Recognizing this need, JPL developed the Airborne Snow Observatory (ASO), an imaging spectrometer and imaging LiDAR system, to quantify snow water equivalent and snow albedo, provide unprecedented knowledge of snow properties, and provide complete, robust inputs to snowmelt runoff models, water management models, and systems of the future. Critical in the design of the ASO system is the availability of snow water equivalent and albedo products within 24 hours of acquisition for timely constraint of snowmelt runoff forecast models. In spring 2013, ASO was deployed for its first year of a multi-year Demonstration Mission of weekly acquisitions in the Tuolumne River Basin (Sierra Nevada) and monthly acquisitions in the Uncompahgre River Basin (Colorado). The ASO data were used to constrain spatially distributed models of varying complexities and integrated into the operations of the O'Shaughnessy Dam on the Hetch Hetchy reservoir on the Tuolumne River. Here we present the first results from the ASO Demonstration Mission 1 along with modeling results with and without the constraint by the ASO's high spatial resolution and spatially

Sensitivity Observing System Experiment (SOSE)-a new effective NWP-based tool in designing the global observing system

NASA Astrophysics Data System (ADS)

Marseille, Gert-Jan; Stoffelen, Ad; Barkmeijer, Jan

2008-03-01

Lacking an established methodology to test the potential impact of prospective extensions to the global observing system (GOS) in real atmospheric cases we developed such a method, called Sensitivity Observing System Experiment (SOSE). For example, since the GOS is non uniform it is of interest to investigate the benefit of complementary observing systems filling its gaps. In a SOSE adjoint sensitivity structures are used to define a pseudo true atmospheric state for the simulation of the prospective observing system. Next, the synthetic observations are used together with real observations from the existing GOS in a state-of-the-art Numerical Weather Prediction (NWP) model to assess the potential added value of the new observing system. Unlike full observing system simulation experiments (OSSE), SOSE can be applied to real extreme events that were badly forecast operationally and only requires the simulation of the new instrument. As such SOSE is an effective tool, for example, to define observation requirements for extensions to the GOS. These observation requirements may serve as input for the design of an operational network of prospective observing systems. In a companion paper we use SOSE to simulate potential future space borne Doppler Wind Lidar (DWL) scenarios and assess their capability to sample meteorologically sensitive areas not well captured by the current GOS, in particular over the Northern Hemisphere oceans.

Jupiter System Observer

NASA Technical Reports Server (NTRS)

Senske, Dave; Kwok, Johnny

2008-01-01

This slide presentation reviews the proposed mission for the Jupiter System Observer. The presentation also includes overviews of the mission timeline, science goals, and spacecraftspecifications for the satellite.

Identification and characterization of intracellular proteins that bind oligonucleotides with phosphorothioate linkages.

PubMed

Liang, Xue-hai; Sun, Hong; Shen, Wen; Crooke, Stanley T

2015-03-11

Although the RNase H-dependent mechanism of inhibition of gene expression by chemically modified antisense oligonucleotides (ASOs) has been well characterized, little is known about the interactions between ASOs and intracellular proteins that may alter cellular localization and/or potency of ASOs. Here, we report the identification of 56 intracellular ASO-binding proteins using multi-step affinity selection approaches. Many of the tested proteins had no significant effect on ASO activity; however, some proteins, including La/SSB, NPM1, ANXA2, VARS and PC4, appeared to enhance ASO activities, likely through mechanisms related to subcellular distribution. VARS and ANXA2 co-localized with ASOs in endocytic organelles, and reduction in the level of VARS altered lysosome/ASO localization patterns, implying that these proteins may facilitate ASO release from the endocytic pathway. Depletion of La and NPM1 reduced nuclear ASO levels, suggesting potential roles in ASO nuclear accumulation. On the other hand, Ku70 and Ku80 proteins inhibited ASO activity, most likely by competition with RNase H1 for ASO/RNA duplex binding. Our results demonstrate that phosphorothioate-modified ASOs bind a set of cellular proteins that affect ASO activity via different mechanisms. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Observing System Evaluations Using GODAE Systems

DTIC Science & Technology

2009-09-01

DISTRIBUTION/AVAILABILITY STATEMENT Approved for public release, distribution is unlimite 13. SUPPLEMENTARY NOTES 20091228151 14. ABSTRACT Global ocean...forecast systems, developed under the Global Ocean Data Assimilation Experiment (GODAE), are a powerful means of assessing the impact of different...components of the Global Ocean Observing System (GOOS). Using a range of analysis tools and approaches, GODAE systems are useful for quantifying the

A Comparison of Different Database Technologies for the CMS AsyncStageOut Transfer Database

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ciangottini, D.; Balcas, J.; Mascheroni, M.

AsyncStageOut (ASO) is the component of the CMS distributed data analysis system (CRAB) that manages users transfers in a centrally controlled way using the File Transfer System (FTS3) at CERN. It addresses a major weakness of the previous, decentralized model, namely that the transfer of the user’s output data to a single remote site was part of the job execution, resulting in inefficient use of job slots and an unacceptable failure rate. Currently ASO manages up to 600k files of various sizes per day from more than 500 users per month, spread over more than 100 sites. ASO uses amore » NoSQL database (CouchDB) as internal bookkeeping and as way to communicate with other CRAB components. Since ASO/CRAB were put in production in 2014, the number of transfers constantly increased up to a point where the pressure to the central CouchDB instance became critical, creating new challenges for the system scalability, performance, and monitoring. This forced a re-engineering of the ASO application to increase its scalability and lowering its operational effort. In this contribution we present a comparison of the performance of the current NoSQL implementation and a new SQL implementation, and how their different strengths and features influenced the design choices and operational experience. We also discuss other architectural changes introduced in the system to handle the increasing load and latency in delivering output to the user.« less

A comparison of different database technologies for the CMS AsyncStageOut transfer database

NASA Astrophysics Data System (ADS)

Ciangottini, D.; Balcas, J.; Mascheroni, M.; Rupeika, E. A.; Vaandering, E.; Riahi, H.; Silva, J. M. D.; Hernandez, J. M.; Belforte, S.; Ivanov, T. T.

2017-10-01

AsyncStageOut (ASO) is the component of the CMS distributed data analysis system (CRAB) that manages users transfers in a centrally controlled way using the File Transfer System (FTS3) at CERN. It addresses a major weakness of the previous, decentralized model, namely that the transfer of the user’s output data to a single remote site was part of the job execution, resulting in inefficient use of job slots and an unacceptable failure rate. Currently ASO manages up to 600k files of various sizes per day from more than 500 users per month, spread over more than 100 sites. ASO uses a NoSQL database (CouchDB) as internal bookkeeping and as way to communicate with other CRAB components. Since ASO/CRAB were put in production in 2014, the number of transfers constantly increased up to a point where the pressure to the central CouchDB instance became critical, creating new challenges for the system scalability, performance, and monitoring. This forced a re-engineering of the ASO application to increase its scalability and lowering its operational effort. In this contribution we present a comparison of the performance of the current NoSQL implementation and a new SQL implementation, and how their different strengths and features influenced the design choices and operational experience. We also discuss other architectural changes introduced in the system to handle the increasing load and latency in delivering output to the user.

Synthetic observations of protostellar multiple systems

NASA Astrophysics Data System (ADS)

Lomax, O.; Whitworth, A. P.

2018-04-01

Observations of protostars are often compared with synthetic observations of models in order to infer the underlying physical properties of the protostars. The majority of these models have a single protostar, attended by a disc and an envelope. However, observational and numerical evidence suggests that a large fraction of protostars form as multiple systems. This means that fitting models of single protostars to observations may be inappropriate. We produce synthetic observations of protostellar multiple systems undergoing realistic, non-continuous accretion. These systems consist of multiple protostars with episodic luminosities, embedded self-consistently in discs and envelopes. We model the gas dynamics of these systems using smoothed particle hydrodynamics and we generate synthetic observations by post-processing the snapshots using the SPAMCART Monte Carlo radiative transfer code. We present simulation results of three model protostellar multiple systems. For each of these, we generate 4 × 104 synthetic spectra at different points in time and from different viewing angles. We propose a Bayesian method, using similar calculations to those presented here, but in greater numbers, to infer the physical properties of protostellar multiple systems from observations.

The Earth Observing System

NASA Technical Reports Server (NTRS)

Shaffer, Lisa Robock

1992-01-01

The restructuring of the NASA Earth Observing System (EOS), designed to provide comprehensive long term observations from space of changes occurring on the Earth from natural and human causes in order to have a sound scientific basis for policy decisions on protection of the future, is reported. In response to several factors, the original program approved in the fiscal year 1991 budget was restructured and somewhat reduced in scope. The resulting program uses three different sized launch vehicles to put six different spacecraft in orbit in the first phase, followed by two replacement launches for each of five of the six satellites to maintain a long term observing capability to meet the needs of global climate change research and other science objectives. The EOS system, including the space observatories, the data and information system, and the interdisciplinary global change research effort, are approved and proceeding. Elements of EOS are already in place, such as the research investigations and initial data system capabilities. The flights of precursor satellite and Shuttle missions, the ongoing data analysis, and the evolutionary enhancements to the integrated Earth science data management capabilities are all important building blocks to the full EOS program.

The Implementation of a Plant and Minor Property Accounting System for the Naval Postgraduate School.

DTIC Science & Technology

1987-09-01

Autmatic Data Processing iquipment CPU Central Procesing Unit DSA Date lase Administrator USU Data "aso Management system D&XC Defense Automation...ground? -How big is a certain piece of plant property (e.g., for shipment)? 2. Nxnected Data Base Volume The current inventory of NPS plant property...System, Automated Data TU I. 89aom so fg o - e In October 136 the aval Postgraduate School was directed to centralize the accounting and control of

Thrombolytic therapeutic effect monitoring based on functional near-infrared spectroscopy

NASA Astrophysics Data System (ADS)

Pan, Boan; Wang, Pengbo; Li, Yaoxian; Gao, Yuan; Li, Ting

2017-02-01

Deep vein thrombosis (DVT) is of serious mortality and morbidity, which often happens in inpatients and especially with the postoperative population [1]. The golden standard to diagnose DVT is venography, which relies on complicated imaging modalities requiring to be injected in a vein below the clot invasively and ionizing procedures that employing xray imaging to show where and how the DVT blocks. The near-infrared spectroscopy (NIRS) is recently found to be an intriguing and potential method detecting DVT in clinics. It has been reported recently that employing NIRS to diagnose DVT. Arteriosclerosis obliterans (ASO), local extremities manifestations of systemic atherosclerosis, usually cause thrombosis and the reduction of distal blood flow. Thrombolytic therapy is to use exogenous activator to activate the dissolution system, which can dissolve intracoronary thrombus. Here we attempt to monitor the DVT and ASO patients during the whole procedure of thrombolytic treatment, then compare the data with those DVT and ASO patients did not take treatments and normal population. 8 DVT and 9 ASO patients and 12 normal subjects were recruited to take the measurements of concentration variation of oxy- and deoxy-hemoglobins (Δ[HbO2] and Δ[Hb]) by NIRS-based thrombosis monitor. Thereinto, 5 DVT and 6 ASO patients has taken the thrombolytic treatment, and the data for the periods before treatment, during treatment, and after treatment were extracted for analysis. We found that Δ[HbO2] fluctuates and even decreases in DVT and ASO patients. After the thrombolytic therapy, Δ[HbO2] increases about 45% and converge to the curves of normal subjects. And the Δ[Hb] emerges the similar trends, except for the rising trend in the beginning and the downtrend after thrombolytic therapy. The findings indicated NIRS has big potential in clinical monitoring of DVT and ASO patients and offering reliable and quantitative evaluation of thrombolytic therapy outcomes.

Autonomous aerial observations to extend and complement the Earth Observing System: a science-driven systems-oriented approach

NASA Astrophysics Data System (ADS)

Sandford, Stephen P.; Harrison, F. W.; Langford, John; Johnson, James W.; Qualls, Garry; Emmitt, David; Jones, W. Linwood; Shugart, Herman H., Jr.

2004-12-01

The current Earth observing capability depends primarily on spacecraft missions and ground-based networks to provide the critical on-going observations necessary for improved understanding of the Earth system. Aircraft missions play an important role in process studies but are limited to relatively short-duration flights. Suborbital observations have contributed to global environmental knowledge by providing in-depth, high-resolution observations that space-based and in-situ systems are challenged to provide; however, the limitations of aerial platforms - e.g., limited observing envelope, restrictions associated with crew safety and high cost of operations have restricted the suborbital program to a supporting role. For over a decade, it has been recognized that autonomous aerial observations could potentially be important. Advances in several technologies now enable autonomous aerial observation systems (AAOS) that can provide fundamentally new observational capability for Earth science and applications and thus lead scientists and engineers to rethink how suborbital assets can best contribute to Earth system science. Properly developed and integrated, these technologies will enable new Earth science and operational mission scenarios with long term persistence, higher-spatial and higher-temporal resolution at lower cost than space or ground based approaches. This paper presents the results of a science driven, systems oriented study of broad Earth science measurement needs. These needs identify aerial mission scenarios that complement and extend the current Earth Observing System. These aerial missions are analogous to space missions in their complexity and potential for providing significant data sets for Earth scientists. Mission classes are identified and presented based on science driven measurement needs in atmospheric, ocean and land studies. Also presented is a nominal concept of operations for an AAOS: an innovative set of suborbital assets that

Linear Multivariable Regression Models for Prediction of Eddy Dissipation Rate from Available Meteorological Data

NASA Technical Reports Server (NTRS)

MCKissick, Burnell T. (Technical Monitor); Plassman, Gerald E.; Mall, Gerald H.; Quagliano, John R.

2005-01-01

Linear multivariable regression models for predicting day and night Eddy Dissipation Rate (EDR) from available meteorological data sources are defined and validated. Model definition is based on a combination of 1997-2000 Dallas/Fort Worth (DFW) data sources, EDR from Aircraft Vortex Spacing System (AVOSS) deployment data, and regression variables primarily from corresponding Automated Surface Observation System (ASOS) data. Model validation is accomplished through EDR predictions on a similar combination of 1994-1995 Memphis (MEM) AVOSS and ASOS data. Model forms include an intercept plus a single term of fixed optimal power for each of these regression variables; 30-minute forward averaged mean and variance of near-surface wind speed and temperature, variance of wind direction, and a discrete cloud cover metric. Distinct day and night models, regressing on EDR and the natural log of EDR respectively, yield best performance and avoid model discontinuity over day/night data boundaries.

76 FR 64235 - Establishment of Class E Airspace; Nahunta, GA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-18

.... * * * * * ASO GA E5 Nahunta, GA [New] Brantley County Airport, GA (Lat. 31[deg]12'22'' N., long. 81[deg]54'22...-0727; Airspace Docket No. 11-ASO-32] Establishment of Class E Airspace; Nahunta, GA AGENCY: Federal... at Nahunta, GA, to accommodate the new Area Navigation (RNAV) Global Positioning System (GPS...

DNA/RNA heteroduplex oligonucleotide for highly efficient gene silencing

PubMed Central

Nishina, Kazutaka; Piao, Wenying; Yoshida-Tanaka, Kie; Sujino, Yumiko; Nishina, Tomoko; Yamamoto, Tsuyoshi; Nitta, Keiko; Yoshioka, Kotaro; Kuwahara, Hiroya; Yasuhara, Hidenori; Baba, Takeshi; Ono, Fumiko; Miyata, Kanjiro; Miyake, Koichi; Seth, Punit P.; Low, Audrey; Yoshida, Masayuki; Bennett, C. Frank; Kataoka, Kazunori; Mizusawa, Hidehiro; Obika, Satoshi; Yokota, Takanori

2015-01-01

Antisense oligonucleotides (ASOs) are recognized therapeutic agents for the modulation of specific genes at the post-transcriptional level. Similar to any medical drugs, there are opportunities to improve their efficacy and safety. Here we develop a short DNA/RNA heteroduplex oligonucleotide (HDO) with a structure different from double-stranded RNA used for short interfering RNA and single-stranded DNA used for ASO. A DNA/locked nucleotide acid gapmer duplex with an α-tocopherol-conjugated complementary RNA (Toc-HDO) is significantly more potent at reducing the expression of the targeted mRNA in liver compared with the parent single-stranded gapmer ASO. Toc-HDO also improves the phenotype in disease models more effectively. In addition, the high potency of Toc-HDO results in a reduction of liver dysfunction observed in the parent ASO at a similar silencing effect. HDO technology offers a novel concept of therapeutic oligonucleotides, and the development of this molecular design opens a new therapeutic field. PMID:26258894

The Global Ocean Observing System

NASA Technical Reports Server (NTRS)

Kester, Dana

1992-01-01

A Global Ocean Observing System (GOOS) should be established now with international coordination (1) to address issues of global change, (2) to implement operational ENSO forecasts, (3) to provide the data required to apply global ocean circulation models, and (4) to extract the greatest value from the one billion dollar investment over the next ten years in ocean remote sensing by the world's space agencies. The objectives of GOOS will focus on climatic and oceanic predictions, on assessing coastal pollution, and in determining the sustainability of living marine resources and ecosystems. GOOS will be a complete system including satellite observations, in situ observations, numerical modeling of ocean processes, and data exchange and management. A series of practical and economic benefits will be derived from the information generated by GOOS. In addition to the marine science community, these benefits will be realized by the energy industries of the world, and by the world's fisheries. The basic oceanic variables that are required to meet the oceanic and predictability objectives of GOOS include wind velocity over the ocean, sea surface temperature and salinity, oceanic profiles of temperature and salinity, surface current, sea level, the extent and thickness of sea ice, the partial pressure of CO2 in surface waters, and the chlorophyll concentration of surface waters. Ocean circulation models and coupled ocean-atmosphere models can be used to evaluate observing system design, to assimilate diverse data sets from in situ and remotely sensed observations, and ultimately to predict future states of the system. The volume of ocean data will increase enormously over the next decade as new satellite systems are launched and as complementary in situ measuring systems are deployed. These data must be transmitted, quality controlled, exchanged, analyzed, and archived with the best state-of-the-art computational methods.

Operable Data Management for Ocean Observing Systems

NASA Astrophysics Data System (ADS)

Chavez, F. P.; Graybeal, J. B.; Godin, M. A.

2004-12-01

As oceanographic observing systems become more numerous and complex, data management solutions must follow. Most existing oceanographic data management systems fall into one of three categories: they have been developed as dedicated solutions, with limited application to other observing systems; they expect that data will be pre-processed into well-defined formats, such as netCDF; or they are conceived as robust, generic data management solutions, with complexity (high) and maturity and adoption rates (low) to match. Each approach has strengths and weaknesses; no approach yet fully addresses, nor takes advantage of, the sophistication of ocean observing systems as they are now conceived. In this presentation we describe critical data management requirements for advanced ocean observing systems, of the type envisioned by ORION and IOOS. By defining common requirements -- functional, qualitative, and programmatic -- for all such ocean observing systems, the performance and nature of the general data management solution can be characterized. Issues such as scalability, maintaining metadata relationships, data access security, visualization, and operational flexibility suggest baseline architectural characteristics, which may in turn lead to reusable components and approaches. Interoperability with other data management systems, with standards-based solutions in metadata specification and data transport protocols, and with the data management infrastructure envisioned by IOOS and ORION, can also be used to define necessary capabilities. Finally, some requirements for the software infrastructure of ocean observing systems can be inferred. Early operational results and lessons learned, from development and operations of MBARI ocean observing systems, are used to illustrate key requirements, choices, and challenges. Reference systems include the Monterey Ocean Observing System (MOOS), its component software systems (Software Infrastructure and Applications for MOOS, and

Therapeutic potential of targeting microRNA-10b in established intracranial glioblastoma: first steps toward the clinic.

PubMed

Teplyuk, Nadiya M; Uhlmann, Erik J; Gabriely, Galina; Volfovsky, Natalia; Wang, Yang; Teng, Jian; Karmali, Priya; Marcusson, Eric; Peter, Merlene; Mohan, Athul; Kraytsberg, Yevgenya; Cialic, Ron; Chiocca, E Antonio; Godlewski, Jakub; Tannous, Bakhos; Krichevsky, Anna M

2016-03-01

MicroRNA-10b (miR-10b) is a unique oncogenic miRNA that is highly expressed in all GBM subtypes, while absent in normal neuroglial cells of the brain. miR-10b inhibition strongly impairs proliferation and survival of cultured glioma cells, including glioma-initiating stem-like cells (GSC). Although several miR-10b targets have been identified previously, the common mechanism conferring the miR-10b-sustained viability of GSC is unknown. Here, we demonstrate that in heterogeneous GSC, miR-10b regulates cell cycle and alternative splicing, often through the non-canonical targeting via 5'UTRs of its target genes, including MBNL1-3, SART3, and RSRC1. We have further assessed the inhibition of miR-10b in intracranial human GSC-derived xenograft and murine GL261 allograft models in athymic and immunocompetent mice. Three delivery routes for the miR-10b antisense oligonucleotide inhibitors (ASO), direct intratumoral injections, continuous osmotic delivery, and systemic intravenous injections, have been explored. In all cases, the treatment with miR-10b ASO led to targets' derepression, and attenuated growth and progression of established intracranial GBM. No significant systemic toxicity was observed upon ASO administration by local or systemic routes. Our results indicate that miR-10b is a promising candidate for the development of targeted therapies against all GBM subtypes. © 2016 The Authors. Published under the terms of the CC BY 4.0 license.

Observing system simulation experiments with multiple methods

NASA Astrophysics Data System (ADS)

Ishibashi, Toshiyuki

2014-11-01

An observing System Simulation Experiment (OSSE) is a method to evaluate impacts of hypothetical observing systems on analysis and forecast accuracy in numerical weather prediction (NWP) systems. Since OSSE requires simulations of hypothetical observations, uncertainty of OSSE results is generally larger than that of observing system experiments (OSEs). To reduce such uncertainty, OSSEs for existing observing systems are often carried out as calibration of the OSSE system. The purpose of this study is to achieve reliable OSSE results based on results of OSSEs with multiple methods. There are three types of OSSE methods. The first one is the sensitivity observing system experiment (SOSE) based OSSE (SOSEOSSE). The second one is the ensemble of data assimilation cycles (ENDA) based OSSE (ENDA-OSSE). The third one is the nature-run (NR) based OSSE (NR-OSSE). These three OSSE methods have very different properties. The NROSSE evaluates hypothetical observations in a virtual (hypothetical) world, NR. The ENDA-OSSE is very simple method but has a sampling error problem due to a small size ensemble. The SOSE-OSSE requires a very highly accurate analysis field as a pseudo truth of the real atmosphere. We construct these three types of OSSE methods in the Japan meteorological Agency (JMA) global 4D-Var experimental system. In the conference, we will present initial results of these OSSE systems and their comparisons.

The Global Observing System in the Assimilation Context

NASA Technical Reports Server (NTRS)

Reinecker, Michele M.; Gelaro, R.; Pawson, S.; Reichle, R.; McCarty, W.

2011-01-01

Weather and climate analyses and predictions all rely on the global observing system. However, the observing system, whether atmosphere, ocean, or land surface, yields a diverse set of incomplete observations of the different components of Earth s environment. Data assimilation systems are essential to synthesize the wide diversity of in situ and remotely sensed observations into four-dimensional state estimates by combining the various observations with model-based estimates. Assimilation, or associated tools and products, are also useful in providing guidance for the evolution of the observing system of the future. This paper provides a brief overview of the global observing system and information gleaned through assimilation tools, and presents some evaluations of observing system gaps and issues.

Cross-species comparison of in vivo PK/PD relationships for second-generation antisense oligonucleotides targeting apolipoprotein B-100.

PubMed

Yu, Rosie Z; Lemonidis, Kristina M; Graham, Mark J; Matson, John E; Crooke, Rosanne M; Tribble, Diane L; Wedel, Mark K; Levin, Arthur A; Geary, Richard S

2009-03-01

The in vivo pharmacokinetics/pharmacodynamics of 2'-O-(2-methoxyethyl) (2'-MOE) modified antisense oligonucleotides (ASOs), targeting apolipoprotein B-100 (apoB-100), were characterized in multiple species. The species-specific apoB antisense inhibitors demonstrated target apoB mRNA reduction in a drug concentration and time-dependent fashion in mice, monkeys, and humans. Consistent with the concentration-dependent decreases in liver apoB mRNA, reductions in serum apoB, and LDL-C, and total cholesterol were concurrently observed in animal models and humans. Additionally, the long duration of effect after cessation of dosing correlated well with the elimination half-life of 2'-MOE modified apoB ASOs studied in mice (t(1/2) congruent with 20 days) and humans (t(1/2) congruent with 30 days) following parental administrations. The plasma concentrations of ISIS 301012, observed in the terminal elimination phase of both mice and monkeys were in equilibrium with liver. The partition ratios between liver and plasma were similar, approximately 6000:1, across species, and thus provide a surrogate for tissue exposure in humans. Using an inhibitory E(max) model, the ASO liver EC(50s) were 101+/-32, 119+/-15, and 300+/-191 microg/g of ASO in high-fat-fed (HF) mice, transgenic mice containing the human apoB transgene, and monkeys, respectively. The estimated liver EC(50) in man, extrapolated from trough plasma exposure, was 81+/-122 microg/g. Therefore, extraordinary consistency of the exposure-response relationship for the apoB antisense inhibitor was observed across species, including human. The cross-species PK/PD relationships provide confidence in the use of pharmacology animal models to predict human dosing for second-generation ASOs targeting the liver.

Biosorption and toxicity responses to arsenite (As[III]) in Scenedesmus quadricauda.

PubMed

Zhang, Jianying; Ding, Tengda; Zhang, Chunlong

2013-08-01

Toxicity and biosorption responses to arsenite (As[III]) were examined in a 96-h exposure study using Scenedesmus quadricauda, one of the most popular green algae distributed in freshwaters in China. Results indicated that the pH-dependent distribution of two arsenite species (H2AsO3(-) and H3AsO3) played an important role in biosorption and toxicity. The undissociated H3AsO3 was more toxic than its monoanionic H2AsO3(-) through comparison of algal cell numbers, chlorophyll-a contents, and algal ultrastructural changes observed with transmission electron microscopy. An effective biosorption of 89.0mgg(-1) at 100mgL(-1) As[III] was found in the treatments with an initial pH of 9.3 and 25.2μgg(-1) at 0.03mgL(-1) As[III] at an initial pH of 8.2 as a result of the predominant species of H2AsO3(-) under the ambient pH and Eh conditions. Our results imply that S. quadricauda may provide a new means for the removal of toxic arsenite species present in contaminated surface water. Copyright © 2013 Elsevier Ltd. All rights reserved.

GEOS observation systems intercomparison investigation results

NASA Technical Reports Server (NTRS)

Berbert, J. H.

1974-01-01

The results of an investigation designed to determine the relative accuracy and precision of the different types of geodetic observation systems used by NASA is presented. A collocation technique was used to minimize the effects of uncertainties in the relative station locations and in the earth's gravity field model by installing accurate reference tracking systems close to the systems to be compared, and by precisely determining their relative survey. The Goddard laser and camera systems were shipped to selected sites, where they tracked the GEOS satellite simultaneously with other systems for an intercomparison observation.

Responses to pandemic ASO3-adjuvanted A/California/07/09 H1N1 influenza vaccine in human immunodeficiency virus-infected individuals.

PubMed

Kelly, Deborah; Burt, Kimberley; Missaghi, Bayan; Barrett, Lisa; Keynan, Yoav; Fowke, Keith; Grant, Michael

2012-08-31

Influenza infection may be more serious in human immunodeficiency virus (HIV)-infected individuals, therefore, vaccination against seasonal and pandemic strains is highly advised. Seasonal influenza vaccines have had no significant negative effects in well controlled HIV infection, but the impact of adjuvanted pandemic A/California/07/2009 H1N1 influenza hemaglutinin (HA) vaccine, which was used for the first time in the Canadian population as an authorized vaccine in autumn 2009, has not been extensively studied. Assess vaccine-related effects on CD4(+) T cell counts and humoral responses to the vaccine in individuals attending the Newfoundland and Labrador Provincial HIV clinic. A single dose of Arepanrix™ split vaccine including 3.75 μg A/California/07/2009 H1N1 HA antigen and ASO3 adjuvant was administered to 81 HIV-infected individuals by intramuscular injection. Plasma samples from shortly before, and 1-5 months after vaccination were collected from 80/81 individuals to assess humoral anti-H1N1 HA responses using a sensitive microbead-based array assay. Data on CD4(+) T cell counts, plasma viral load, antiretroviral therapy and patient age were collected from clinical records of 81 individuals. Overall, 36/80 responded to vaccination either by seroconversion to H1N1 HA or with a clear increase in anti-H1N1 HA antibody levels. Approximately 1/3 (28/80) had pre-existing anti-H1N1 HA antibodies and were more likely to respond to vaccination (22/28). Responders had higher baseline CD4(+) T cell counts and responders without pre-existing antibodies against H1N1 HA were younger than either non-responders or responders with pre-existing antibodies. Compared to changes in their CD4(+) T cell counts observed over a similar time period one year later, vaccine recipients displayed a minor, transient fall in CD4(+) T cell numbers, which was greater amongst responders. We observed low response rates to the 2009 pandemic influenza vaccine among HIV-infected individuals

Co-Administration of an Excipient Oligonucleotide Helps Delineate Pathways of Productive and Nonproductive Uptake of Phosphorothioate Antisense Oligonucleotides in the Liver.

PubMed

Donner, Aaron J; Wancewicz, Edward V; Murray, Heather M; Greenlee, Sarah; Post, Noah; Bell, Melanie; Lima, Walt F; Swayze, Eric E; Seth, Punit P

2017-08-01

Phosphorothioate (PS) modified antisense oligonucleotides (ASOs) have progressed rapidly in the clinic for treating a variety of disease indications. We previously demonstrated that the activity of PS ASOs in the liver can be enhanced by co-infusion of an excipient oligonucleotide (EON). It was posited that the EON saturates a nonproductive uptake pathway(s) thereby permitting accumulation of the PS ASO in a productive tissue compartment. In this report, we measured PS ASO activity following administration by bolus, infusion or co-fusion with EON within hepatocytes and nonparenchymal cells (NPCs), of the liver. This revealed that while ASOs accumulate preferentially in NPCs, they are intrinsically more active in hepatocytes. Furthermore, we show that the EON enhances ASO potency when infused up to 72 h before or after administration of the active ASO suggesting that the EON can saturate and displace the ASO from nonproductive to productive compartments. Physical presence of the EON in tissues was required for optimal potentiation suggesting that there is a dynamic distribution of the ASO and EON between the compartments. Lastly, using a candidate approach, we confirmed Stabilin-2 as a molecular pathway for ASO uptake in sinusoidal endothelial cells and the ASGR as a pathway for ASO uptake into hepatocytes in the liver.

Observing System Simulation Experiments

NASA Technical Reports Server (NTRS)

Prive, Nikki

2015-01-01

This presentation gives an overview of Observing System Simulation Experiments (OSSEs). The components of an OSSE are described, along with discussion of the process for validating, calibrating, and performing experiments. a.

Solar System Observations with JWST

NASA Technical Reports Server (NTRS)

Norwood, James; Hammel, Heidi; Milam, Stefanie; Stansberry, John; Lunine, Jonathan; Chanover, Nancy; Hines, Dean; Sonneborn, George; Tiscareno, Matthew; Brown, Michael;

Using Combined Marine Spatial Planning Tools and Observing System Experiments to define Gaps in the Emerging European Ocean Observing System.

NASA Astrophysics Data System (ADS)

Nolan, G.; Pinardi, N.; Vukicevic, T.; Le Traon, P. Y.; Fernandez, V.

2016-02-01

Ocean observations are critical to providing accurate ocean forecasts that support operational decision making in European open and coastal seas. Observations are available in many forms from Fixed platforms e.g. Moored Buoys and tide gauges, underway measurements from Ferrybox systems, High Frequency radars and more recently from underwater Gliders and profiling floats. Observing System Simulation Experiments have been conducted to examine the relative contribution of each type of platform to an improvement in our ability to accurately forecast the future state of the ocean with HF radar and Gliders showing particular promise in improving model skill. There is considerable demand for ecosystem products and services from today's ocean observing system and biogeochemical observations are still relatively sparse particularly in coastal and shelf seas. There is a need to widen the techniques used to assess the fitness for purpose and gaps in the ocean observing system. As well as Observing System Simulation Experiments that quantify the effect of observations on the overall model skill we present a gap analysis based on (1) Examining where high model skill is required based on a marine spatial planning analysis of European seas i.e where does activity take place that requires more accurate forecasts? and (2) assessing gaps based on the capacity of the observing system to answer key societal challenges e.g. site suitability for aquaculture and ocean energy, oil spill response and contextual oceanographic products for fisheries and ecosystems. The broad based analysis will inform the development of the proposed European Ocean Observing System as a contribution to the Global Ocean Observing System (GOOS).

Carrier Based Air Logistics Study: Maintenance Analysis.

DTIC Science & Technology

1982-01-01

MONITORING AGENCY NAME & ADDRESS(If dIierent loan Controling 01116.) 1S. SECURITY CLASS. (of Od. report) gel Unclassified IS&. DECL ASSI IlCATION/ OOWNGRAOIN...Management System AECL Avionics Equipment Configuration List AIMD Aircraft Intermediate Maintenance Department ASO Aviation Supply Office ASW...implementation. Component-specific data, and indentured[2] relationships between components extracted from the Aviation Supply Office ( ASO ) weapon

Synthesis and Assimilation Systems - Essential Adjuncts to the Global Ocean Observing System

NASA Technical Reports Server (NTRS)

Rienecker, Michele M.; Balmaseda, Magdalena; Awaji, Toshiyuki; Barnier, Bernard; Behringer, David; Bell, Mike; Bourassa, Mark; Brasseur, Pierre; Breivik, Lars-Anders; Carton, James;

Expanded science and management utllity of SWE and albedo data from the NASA/JPL Airborne Snow Observatory

NASA Astrophysics Data System (ADS)

Painter, T. H.; Deems, J. S.; Marks, D. G.; Hedrick, A. R.; Bormann, K.; Skiles, S. M.; Boardman, J. W.; Graham, C. B.; McGurk, B. J.; Gehrke, F.; Berisford, D. F.; Ferraz, A.; Saatchi, S.; Schimel, D.

2016-12-01

The NASA Airborne Snow Observatory (ASO), an imaging spectrometer and imaging LiDAR system, to quantify snow water equivalent and snow albedo, provide unprecedented knowledge of snow properties, and provide complete, robust inputs to snowmelt runoff models, water management models, and systems of the future. This talk presents results from the fourth year of the ASO program, 2016, and the now four years of data record in the Western United States. Following on the heels of the most intense, sustained drought in California history, 2016 held promise of a large snowfall year due to an intense El Nino anomaly. Ultimately, the year had approximately 85% of average peak SWE. In the Sierra Nevada, ASO measured 10x greater SWE than near peak in the dramatic 2015 drought year, and twice that of the more moderate drought year of 2013. Water managers in the Sierra were using these data regularly and extending the dynamic range of newly established relationships between accumulated runoff (circa April through July runoff) and ASO total basin SWE acquisitions. ASO also participated in the NASA OLYMPEX project by flying the entire snow-covered reghions of the Olympic Peninsula for distributed SWE. These data are now being used to validate snowfall estimates from modeling and accumulation patterns as inferred from the NASA Global Precipitation Measurement mission (GPM). The ASO snow program expanded to acquire data in the McKenzie and Deschutes Rivers of Oregon in participation with university and state/federal agencies; Sagehen and Lee Vining basins in the Sierra Nevada, California; the Reynolds Creek Experimental Watershed in Idaho; and the East River, in the Colorado River Basin. These regions extend the existing program flying the Tuolumne, Merced, Lakes, Rush Creek, and Middle+South Forks of Kings River Basins in the California Sierra Nevada and the Upper Rio Grande, Conejos, and Uncompahgre Basins in the Colorado Rocky Mountains.The ASO SWE and albedo data are now being

NOAA Observing System Integrated Analysis (NOSIA): development and support to the NOAA Satellite Observing System Architecture

NASA Astrophysics Data System (ADS)

Reining, R. C.; Cantrell, L. E., Jr.; Helms, D.; LaJoie, M.; Pratt, A. S.; Ries, V.; Taylor, J.; Yuen-Murphy, M. A.

2016-12-01

There is a deep relationship between NOSIA-II and the Federal Earth Observation Assessment (EOA) efforts (EOA 2012 and 2016) chartered under the National Science and Technology Council, Committee on Environment, Natural Resources, and Sustainability, co-chaired by the White House Office of Science and Technology Policy, NASA, NOAA, and USGS. NOSIA-1, which was conducted with a limited scope internal to NOAA in 2010, developed the methodology and toolset that was adopted for EOA 2012, and NOAA staffed the team that conducted the data collection, modeling, and analysis effort for EOA 2012. EOA 2012 was the first-ever integrated analysis of the relative impact of 379 observing systems and data sources contributing to the key objectives identified for 13 Societal Benefit Areas (SBA) including Weather, Climate, Disasters, Oceans and Coastal Resources, and Water Resources. This effort culminated in the first National Plan for Civil Earth Observations. NOAA conducted NOSIA-II starting in 2012 to extend the NOSIA methodology across all of NOAA's Mission Service Areas, covering a representative sample (over 1000) of NOAA's products and services. The detailed information from NOSIA-II is being integrated into EOA 2016 to underpin a broad array of Key Products, Services, and (science) Objectives (KPSO) identified by the inter-agency SBA teams. EOA 2016 is expected to provide substantially greater insight into the cross-agency impacts of observing systems contributing to a wide array of KPSOs, and by extension, to societal benefits flowing from these public-facing products. NOSIA-II is being adopted by NOAA as a corporate decision-analysis and support capability to inform leadership decisions on its integrated observing systems portfolio. Application examples include assessing the agency-wide impacts of planned decommissioning of ships and aircraft in NOAA's fleet, and the relative cost-effectiveness of alternative space-based architectures in the post-GOES-R and JPSS era

Integrated Arctic Observation System Development Under Horizon 2020

NASA Astrophysics Data System (ADS)

Sandven, S.

2016-12-01

The overall objective of INTAROS is to develop an integrated Arctic Observation System (iAOS) by extending, improving and unifying existing systems in the different regions of the Arctic. INTAROS will have a strong multidisciplinary focus, with tools for integration of data from atmosphere, ocean, cryosphere and terrestrial sciences, provided by institutions in Europe, North America and Asia. Satellite earth observation data plays an increasingly important role in such observing systems, because the amount of EO data for observing the global climate and environment grows year by year. In situ observing systems are much more limited due to logistical constraints and cost limitations. The sparseness of in situ data is therefore the largest gap in the overall observing system. INTAROS will assess strengths and weaknesses of existing observing systems and contribute with innovative solutions to fill some of the critical gaps in the in situ observing network. INTAROS will develop a platform, iAOS, to search for and access data from distributed databases. The evolution into a sustainable Arctic observing system requires coordination, mobilization and cooperation between the existing European and international infrastructures (in-situ and remote including space-based), the modeling communities and relevant stakeholder groups. INTAROS will include development of community-based observing systems, where local knowledge is merged with scientific data. An integrated Arctic Observation System will enable better-informed decisions and better-documented processes within key sectors (e.g. local communities, shipping, tourism, fishing), in order to strengthen the societal and economic role of the Arctic region and support the EU strategy for the Arctic and related maritime and environmental policies.

Earth observing system: 1989 reference handbook

NASA Technical Reports Server (NTRS)

1989-01-01

NASA is studying a coordinated effort called the Mission to Planet Earth to understand global change. The goals are to understand the Earth as a system, and to determine those processes that contribute to the environmental balance, as well as those that may result in changes. The Earth Observing System (Eos) is the centerpiece of the program. Eos will create an integrated scientific observing system that will enable multidisciplinary study of the Earth including the atmosphere, oceans, land surface, polar regions, and solid Earth. Science goals, the Eos data and information system, experiments, measuring instruments, and interdisciplinary investigations are described.

Transpiration characteristics of forests and shrubland under land cover change within the large caldera of Mt. Aso, Japan

NASA Astrophysics Data System (ADS)

Miyazawa, Y.; Inoue, A.; Maruyama, A.

2013-12-01

Grassland within a caldera of Mt. Aso has been maintained for fertilizer production from grasses and cattle feeding. Due to the changes in the agricultural and social structure since 1950's, a large part of the grassland was converted to plantations or abandoned to shrublands. Because vegetations of different plant functional types differ in evapotranspiration; ET, a research project was launched to examine the effects of the ongoing land use change on the ET within the caldera, and consequently affect the surface and groundwater discharge of the region. As the part of the project, transpiration rate; E of the major 3 forest types were investigated using sap flow measurements. Based on the measured data, stomatal conductance; Gs was inversely calculated and its response to the environmental factors was modeled using Jarvis-type equation in order to estimate ET of a given part of the caldera based on the plant functional type and the weather data. The selected forests were conifer plantation, deciduous broadleaved plantation and shrubland, which were installed with sap flow sensors to calculate stand-level transpiration rate. Sap flux; Js did not show clear differences among sites despite the large differences in sapwood area. In early summer solar radiation was limited to low levels due to frequent rainfall events and therefore, Js was the function of solar radiation rather than other environmental factors, such as vapor pressure deficit and soil water content. Gs was well regressed with the vapor pressure deficit and solar radiation. The estimated E based on Gs model and the weather data was 0.3-1.2 mm day-1 for each site and was comparable to the E of grassland in other study sites. Results suggested that transpiration rate in growing was not different between vegetations but its annual value are thought to differ due to the different phenology.

Characterization and Application of a Nose-Only Exposure Chamber for Inhalation Delivery of Liposomal Drugs and Nucleic Acids to Mice

PubMed Central

Seshadri, S.; Garbuzenko, O.B.; Han, T.; Wang, Z.; Minko, T.

2013-01-01

Abstract Background A small nose-only exposure chamber was evaluated for inhalation delivery of drug carrier systems (DCSs) to mice for the treatment of lung cancer. The chamber then was used for inhalation delivery of an anticancer drug, antisense oligonucleotides (ASO), and small interfering RNA (siRNA) directly to the cancerous lungs of mice. Methods The uniformity of particle delivery across the ports of the exposure chamber and stability of the DCS (liposomes) during continuous aerosolization by a Collison nebulizer were examined. The mean produced particle size by number was approximately 130 nm, and the mass median diameter was approximately 270 nm. The system was then used to deliver DCS containing doxorubicin (DOX) and ASO or siRNA targeted to multidrug resistance-associated protein 1 (MRP1) mRNA as suppressors of cancer cell resistance. The retention of the drug in the lungs and the effect on tumor size were compared after inhalation delivery and intravenous injection in a nu/nu mouse model of lung cancer. Results The aerosol mass across the four inhalation ports had a coefficient of variation of less than 12%, and approximately 1.4% of the nebulized mass was available for inhalation at each port. The mean size of 130 nm of liposomal DCS did not change significantly during continuous 60-min aerosolization. For inhalation delivery of DCS with DOX+ASO/siRNA, the amount of drugs available for inhalation was lower compared with intravenous injection of DOX; however, the observed lung dose and the retention time were significantly higher. The delivery of DOX+ASO/siRNA via inhalation resulted in tumor volume reduction of more than 90%, whereas only about 40% reduction was achieved after intravenous injection of DOX. Conclusions The investigated exposure system is suitable for inhalation delivery of complex DCS, and its use to deliver DCS containing anticancer drugs and resistance suppressors via inhalation offered a superior method for lung cancer

Observing System Simulation Experiments: An Overview

NASA Technical Reports Server (NTRS)

Prive, Nikki C.; Errico, Ronald M.

2016-01-01

An overview of Observing System Simulation Experiments (OSSEs) will be given, with focus on calibration and validation of OSSE frameworks. Pitfalls and practice will be discussed, including observation error characteristics, incestuousness, and experimental design. The potential use of OSSEs for investigation of the behaviour of data assimilation systems will be explored, including some results from experiments using the NASAGMAO OSSE.

A Convex Formulation for Learning a Shared Predictive Structure from Multiple Tasks

PubMed Central

Chen, Jianhui; Tang, Lei; Liu, Jun; Ye, Jieping

2013-01-01

In this paper, we consider the problem of learning from multiple related tasks for improved generalization performance by extracting their shared structures. The alternating structure optimization (ASO) algorithm, which couples all tasks using a shared feature representation, has been successfully applied in various multitask learning problems. However, ASO is nonconvex and the alternating algorithm only finds a local solution. We first present an improved ASO formulation (iASO) for multitask learning based on a new regularizer. We then convert iASO, a nonconvex formulation, into a relaxed convex one (rASO). Interestingly, our theoretical analysis reveals that rASO finds a globally optimal solution to its nonconvex counterpart iASO under certain conditions. rASO can be equivalently reformulated as a semidefinite program (SDP), which is, however, not scalable to large datasets. We propose to employ the block coordinate descent (BCD) method and the accelerated projected gradient (APG) algorithm separately to find the globally optimal solution to rASO; we also develop efficient algorithms for solving the key subproblems involved in BCD and APG. The experiments on the Yahoo webpages datasets and the Drosophila gene expression pattern images datasets demonstrate the effectiveness and efficiency of the proposed algorithms and confirm our theoretical analysis. PMID:23520249

CATOS (Computer Aided Training/Observing System): Automating animal observation and training.

PubMed

Oh, Jinook; Fitch, W Tecumseh

2017-02-01

In animal behavioral biology, an automated observing/training system may be useful for several reasons: (a) continuous observation of animals for documentation of specific, irregular events, (b) long-term intensive training of animals in preparation for behavioral experiments, (c) elimination of potential cues and biases induced by humans during training and testing. Here, we describe an open-source-based system named CATOS (Computer Aided Training/Observing System) developed for such situations. There are several notable features in this system. CATOS is flexible and low cost because it is based on free open-source software libraries, common hardware parts, and open-system electronics based on Arduino. Automated video condensation is applied, leading to significantly reduced video data storage compared to the total active hours of the system. A data-viewing utility program helps a user browse recorded data quickly and more efficiently. With these features, CATOS has the potential to be applied to many different animal species in various environments such as laboratories, zoos, or even private homes. Also, an animal's free access to the device without constraint, and a gamified learning process, enhance the animal's welfare and enriches their environment. As a proof of concept, the system was built and tested with two different species. Initially, the system was tested for approximately 10 months with a domesticated cat. The cat was successfully and fully automatically trained to discriminate three different spoken words. Then, in order to test the system's adaptability to other species and hardware components, we used it to train a laboratory rat for 3 weeks.

An adaptive tracking observer for failure-detection systems

NASA Technical Reports Server (NTRS)

Sidar, M.

1982-01-01

The design problem of adaptive observers applied to linear, constant and variable parameters, multi-input, multi-output systems, is considered. It is shown that, in order to keep the observer's (or Kalman filter) false-alarm rate (FAR) under a certain specified value, it is necessary to have an acceptable proper matching between the observer (or KF) model and the system parameters. An adaptive observer algorithm is introduced in order to maintain desired system-observer model matching, despite initial mismatching and/or system parameter variations. Only a properly designed adaptive observer is able to detect abrupt changes in the system (actuator, sensor failures, etc.) with adequate reliability and FAR. Conditions for convergence for the adaptive process were obtained, leading to a simple adaptive law (algorithm) with the possibility of an a priori choice of fixed adaptive gains. Simulation results show good tracking performance with small observer output errors and accurate and fast parameter identification, in both deterministic and stochastic cases.

15 CFR Appendix A to Part 946 - National Weather Service Modernization Criteria

Code of Federal Regulations, 2010 CFR

2010-01-01

... specifically in Addendum I, Appendix D of the ASOS Site Component Commissioning Evaluation Package (the ASOS Package). Criteria: a. ASOS Acceptance Test: The site component acceptance test, which includes objective..., has been successfully completed in accordance with item 1a, p. D-2 of Appendix D of the ASOS Package...

15 CFR Appendix A to Part 946 - National Weather Service Modernization Criteria

Code of Federal Regulations, 2013 CFR

2013-01-01

... specifically in Addendum I, Appendix D of the ASOS Site Component Commissioning Evaluation Package (the ASOS Package). Criteria: a. ASOS Acceptance Test: The site component acceptance test, which includes objective..., has been successfully completed in accordance with item 1a, p. D-2 of Appendix D of the ASOS Package...

15 CFR Appendix A to Part 946 - National Weather Service Modernization Criteria

Code of Federal Regulations, 2012 CFR

2012-01-01

... specifically in Addendum I, Appendix D of the ASOS Site Component Commissioning Evaluation Package (the ASOS Package). Criteria: a. ASOS Acceptance Test: The site component acceptance test, which includes objective..., has been successfully completed in accordance with item 1a, p. D-2 of Appendix D of the ASOS Package...

15 CFR Appendix A to Part 946 - National Weather Service Modernization Criteria

Code of Federal Regulations, 2011 CFR

2011-01-01

... specifically in Addendum I, Appendix D of the ASOS Site Component Commissioning Evaluation Package (the ASOS Package). Criteria: a. ASOS Acceptance Test: The site component acceptance test, which includes objective..., has been successfully completed in accordance with item 1a, p. D-2 of Appendix D of the ASOS Package...

Assessing Preschool Children's Competitive Behaviour: An Observational System

ERIC Educational Resources Information Center

Tsiakara, Angeliki; Digelidis, Nikolaos M.

2014-01-01

The aim of this study was to develop a direct observational system in order to assess competitive behaviours in preschool children. Participants were 176 children (90 boys, 86 girls; M[subscript age]?=?5.2 years) from 10 kindergarten classes of one town of Central Greece. A new observational system (Observational System Assessing Competition in…

Development of quality metrics for ambulatory pediatric cardiology: Transposition of the great arteries after arterial switch operation.

PubMed

Baker-Smith, Carissa M; Carlson, Karina; Ettedgui, Jose; Tsuda, Takeshi; Jayakumar, K Anitha; Park, Matthew; Tede, Nikola; Uzark, Karen; Fleishman, Craig; Connuck, David; Likes, Maggie; Penny, Daniel J

2018-01-01

To develop quality metrics (QMs) for the ambulatory care of patients with transposition of the great arteries following arterial switch operation (TGA/ASO). Under the auspices of the American College of Cardiology Adult Congenital and Pediatric Cardiology (ACPC) Steering committee, the TGA/ASO team generated candidate QMs related to TGA/ASO ambulatory care. Candidate QMs were submitted to the ACPC Steering Committee and were reviewed for validity and feasibility using individual expert panel member scoring according to the RAND-UCLA methodology. QMs were then made available for review by the entire ACC ACPC during an "open comment period." Final approval of each QM was provided by a vote of the ACC ACPC Council. Patients with TGA who had undergone an ASO were included. Patients with complex transposition were excluded. Twelve candidate QMs were generated. Seven metrics passed the RAND-UCLA process. Four passed the "open comment period" and were ultimately approved by the Council. These included: (1) at least 1 echocardiogram performed during the first year of life reporting on the function, aortic dimension, degree of neoaortic valve insufficiency, the patency of the systemic and pulmonary outflows, the patency of the branch pulmonary arteries and coronary arteries, (2) neurodevelopmental (ND) assessment after ASO; (3) lipid profile by age 11 years; and (4) documentation of a transition of care plan to an adult congenital heart disease (CHD) provider by 18 years of age. Application of the RAND-UCLA methodology and linkage of this methodology to the ACPC approval process led to successful generation of 4 QMs relevant to the care of TGA/ASO pediatric patients in the ambulatory setting. These metrics have now been incorporated into the ACPC Quality Network providing guidance for the care of TGA/ASO patients across 30 CHD centers. © 2017 Wiley Periodicals, Inc.

Antitumor activity of Annona squamosa seed oil.

PubMed

Chen, Yong; Chen, Yayun; Shi, Yeye; Ma, Chengyao; Wang, Xunan; Li, Yue; Miao, Yunjie; Chen, Jianwei; Li, Xiang

2016-12-04

Custard apple (Annona squamosa Linn.) is an edible tropical fruit, and its seeds have been used to treat "malignant sore" (cancer) and other usage as insecticide. A comparison of extraction processes, chemical composition analysis and antitumor activity of A. squamosa seed oil (ASO) were investigated. The optimal extraction parameters of ASO were established by comparing percolation, soxhlet, ultrasonic and SFE-CO 2 extraction methods. The chemical composition of fatty acid and content of total annonaceous acetogenins (ACGs) of ASO was investigated by GC-MS and colorimetric assay, and anti-tumor activity of ASO was tested using H 22 xenografts bearing mice. The optimal extraction parameters of ASO were obtained as follows: using soxhlet extraction method with extraction solvent of petroleum ether, temperature of 80°C, and extraction time of 90min. Under these conditions, the yield of ASO was 22.65%. GC-MS analysis results showed that the main chemical compositions of fatty acid of ASO were palmitic acid (9.92%), linoleic acid (20.49%), oleic acid (56.50%) and stearic acid (9.14%). The total ACGs content in ASO was 41.00mg/g. ASO inhibited the growth of H 22 tumor cells in mice with a maximum inhibitory rate of 53.54% by oral administration. Furthermore, it was found that ASO exerted an antitumor effect via decreasing interleukin-6 (IL-6), janus kinase (Jak) and phosphorylated signal transducers and activators of transcription (p-Stat3) expression. The results demonstrated that ASO suppressed the H 22 solid tumor development may due to its main chemical constituents unsaturated fatty acid and ACGs via IL-6/Jak/Stat3 pathway. ASO may be a potential candidate for the treatment of cancer. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Metabolic Consequences of High-Fat Diet Are Attenuated by Suppression of HIF-1α

PubMed Central

Shin, Mi-Kyung; Drager, Luciano F.; Yao, Qiaoling; Bevans-Fonti, Shannon; Yoo, Doo-Young; Jun, Jonathan C.; Aja, Susan; Bhanot, Sanjay; Polotsky, Vsevolod Y.

2012-01-01

Obesity is associated with tissue hypoxia and the up-regulation of hypoxia inducible factor 1 alpha (HIF-1α). Prior studies in transgenic mice have shown that HIF-1α plays a role in the metabolic dysfunction associated with obesity. Therefore, we hypothesized that, after the development of diet-induced obesity (DIO), metabolic function could be improved by administration of HIF-1α antisense oligonucleotides (ASO). DIO mice were treated with HIF-1α ASO or with control ASO for 8 weeks and compared with an untreated group. We found that HIF-1α ASO markedly suppressed Hif-1α gene expression in adipose tissue and the liver. HIF-1α ASO administration induced weight loss. Final body weight was 41.6±1.4 g in the HIF-1α ASO group vs 46.7±0.9 g in the control ASO group and 47.9±0.8 g in untreated mice (p<0.001). HIF-1α ASO increased energy expenditure (13.3±0.6 vs 12±0.1 and 11.9±0.4 kcal/kg/hr, respectively, p<0.001) and decreased the respiratory exchange ratio (0.71±0.01 vs 0.75±0.01 and 0.76±0.01, respectively, p<0.001), which suggested switching metabolism to fat oxidation. In contrast, HIF-1a ASO had no effect on food intake or activity. HIF-1α ASO treatment decreased fasting blood glucose (195.5±8.4 mg/dl vs 239±7.8 mg/dl in the control ASO group and 222±8.2 mg/dl in untreated mice, p<0.01), plasma insulin, hepatic glucose output, and liver fat content. These findings demonstrate that the metabolic consequences of DIO are attenuated by HIF-1α ASO treatment. PMID:23049707

Land-cover observations as part of a Global Earth Observation System of Systems (GEOSS): Progress, activities, and prospects

USGS Publications Warehouse

Herold, M.; Woodcock, C.E.; Loveland, Thomas R.; Townshend, J.; Brady, M.; Steenmans, C.; Schmullius, C. C.

2008-01-01

The international land-cover community has been working with GEO since 2005 to build the foundations for land-cover observations as an integral part of a Global Earth Observation System of Systems (GEOSS). The Group on Earth Observation (GEO) has provided the platform to elevate the societal relevance of land cover monitoring and helped to link a diverse set of global, regional, and national activities. A dedicated 2007-2009 GEO work plan task has resulted in achievements on the strategic and implementation levels. Integrated Global Observations of the Land (IGOL), the land theme of the Integrated Global Observation Strategy (IGOS), has been approved and is now in the process of transition into GEO implementation. New global land-cover maps at moderate spatial resolutions (i.e., GLOBCOVER) are being produced using guidelines and standards of the international community. The Middecadal Global Landsat Survey for 2005-2006 is extending previous 1990 and 2000 efforts for global, high-quality Landsat data. Despite this progress, essential challenges for building a sustained global land-cover-observing system remain, including: international cooperation on the continuity of global observations; ensuring consistency in land monitoring approaches; community engagement and country participation in mapping activities; commitment to ongoing quality assurance and validation; and regional networking and capacity building.

1993 Earth Observing System reference handbook

NASA Technical Reports Server (NTRS)

Asrar, Ghassem (Editor); Dokken, David Jon (Editor)

1993-01-01

Mission to Planet Earth (MTPE) is a NASA-sponsored concept that uses space- and ground-based measurement systems to provide the scientific basis for understanding global change. The space-based components of MTPE will provide a constellation of satellites to monitor the Earth from space. Sustained observations will allow researchers to monitor climate variables overtime to determine trends; however, space-based monitoring alone is not sufficient. A comprehensive data and information system, a community of scientists performing research with the data acquired, and extensive ground campaigns are all important components. Brief descriptions of the various elements that comprise the overall mission are provided. The Earth Observing System (EOS) - a series of polar-orbiting and low-inclination satellites for long-term global observations of the land surface, biosphere, solid Earth, atmosphere, and oceans - is the centerpiece of MTPE. The elements comprising the EOS mission are described in detail.

On the development of earth observation satellite systems

NASA Technical Reports Server (NTRS)

1977-01-01

Subsequent to the launching of the first LANDSAT by NASA, Japan has recognized the importance of data from earth observation satellites, has conducted studies, and is preparing to develop an independent system. The first ocean observation satellite will be launched in 1983, the second in 1985. The first land observation satellite is scheduled to be launched in 1987 and by 1990 Japan intends to have both land and ocean observation systems in regular operation. The association reception and data processing systems are being developed.

Therapeutic suppression of translation initiation factor eIF4E expression reduces tumor growth without toxicity

PubMed Central

Graff, Jeremy R.; Konicek, Bruce W.; Vincent, Thomas M.; Lynch, Rebecca L.; Monteith, David; Weir, Spring N.; Schwier, Phil; Capen, Andrew; Goode, Robin L.; Dowless, Michele S.; Chen, Yuefeng; Zhang, Hong; Sissons, Sean; Cox, Karen; McNulty, Ann M.; Parsons, Stephen H.; Wang, Tao; Sams, Lillian; Geeganage, Sandaruwan; Douglass, Larry E.; Neubauer, Blake Lee; Dean, Nicholas M.; Blanchard, Kerry; Shou, Jianyong; Stancato, Louis F.; Carter, Julia H.; Marcusson, Eric G.

2007-01-01

Expression of eukaryotic translation initiation factor 4E (eIF4E) is commonly elevated in human and experimental cancers, promoting angiogenesis and tumor growth. Elevated eIF4E levels selectively increase translation of growth factors important in malignancy (e.g., VEGF, cyclin D1) and is thereby an attractive anticancer therapeutic target. Yet to date, no eIF4E-specific therapy has been developed. Herein we report development of eIF4E-specific antisense oligonucleotides (ASOs) designed to have the necessary tissue stability and nuclease resistance required for systemic anticancer therapy. In mammalian cultured cells, these ASOs specifically targeted the eIF4E mRNA for destruction, repressing expression of eIF4E-regulated proteins (e.g., VEGF, cyclin D1, survivin, c-myc, Bcl-2), inducing apoptosis, and preventing endothelial cells from forming vessel-like structures. Most importantly, intravenous ASO administration selectively and significantly reduced eIF4E expression in human tumor xenografts, significantly suppressing tumor growth. Because these ASOs also target murine eIF4E, we assessed the impact of eIF4E reduction in normal tissues. Despite reducing eIF4E levels by 80% in mouse liver, eIF4E-specific ASO administration did not affect body weight, organ weight, or liver transaminase levels, thereby providing the first in vivo evidence that cancers may be more susceptible to eIF4E inhibition than normal tissues. These data have prompted eIF4E-specific ASO clinical trials for the treatment of human cancers. PMID:17786246

Interleaved Observation Execution and Rescheduling on Earth Observing Systems

NASA Technical Reports Server (NTRS)

Khatib, Lina; Frank, Jeremy; Smith, David; Morris, Robert; Dungan, Jennifer

2003-01-01

Observation scheduling for Earth orbiting satellites solves the following problem: given a set of requests for images of the Earth, a set of instruments for acquiring those images distributed on a collecting of orbiting satellites, and a set of temporal and resource constraints, generate a set of assignments of instruments and viewing times to those requests that satisfy those constraints. Observation scheduling is often construed as a constrained optimization problem with the objective of maximizing the overall utility of the science data acquired. The utility of an image is typically based on the intrinsic importance of acquiring it (for example, its importance in meeting a mission or science campaign objective) as well as the expected value of the data given current viewing conditions (for example, if the image is occluded by clouds, its value is usually diminished). Currently, science observation scheduling for Earth Observing Systems is done on the ground, for periods covering a day or more. Schedules are uplinked to the satellites and are executed rigorously. An alternative to this scenario is to do some of the decision-making about what images are to be acquired on-board. The principal argument for this capability is that the desirability of making an observation can change dynamically, because of changes in meteorological conditions (e.g. cloud cover), unforeseen events such as fires, floods, or volcanic eruptions, or un-expected changes in satellite or ground station capability. Furthermore, since satellites can only communicate with the ground between 5% to 10% of the time, it may be infeasible to make the desired changes to the schedule on the ground, and uplink the revisions in time for the on-board system to execute them. Examples of scenarios that motivate an on-board capability for revising schedules include the following. First, if a desired visual scene is completely obscured by clouds, then there is little point in taking it. In this case

Th(As(III)4As(V)4O18): a mixed-valent oxoarsenic(III)/arsenic(V) actinide compound obtained under extreme conditions.

PubMed

Yu, Na; Klepov, Vladislav V; Kegler, Philip; Bosbach, Dirk; Albrecht-Schmitt, Thomas E; Alekseev, Evgeny V

2014-08-18

A high-temperature/high-pressure method was employed to investigate phase formation in the Th(NO3)4·5H2O-As2O3-CsNO3 system. It was observed that an excess of arsenic(III) in starting system leads to the formation of Th(As(III)4As(V)4O18), which is representative of a rare class of mixed-valent arsenic(III)/arsenic(V) compounds. This compound was studied with X-ray diffraction, energy-dispersive X-ray, and Raman spectroscopy methods. Crystallographic data show that Th(As(III)4As(V)4O18) is built from (As(III)4As(V)4O18)(4-) layers connected through Th atoms. The arsenic layers are found to be isoreticular to those in previously reported As2O3 and As3O5(OH), and the geometric differences between them are discussed. Bands in the Raman spectrum are assigned with respect to the presence of AsO3 and AsO4 groups.

Hemimorphite as a natural sink for arsenic in zinc deposits and related mine tailings: Evidence from single-crystal EPR spectroscopy and hydrothermal synthesis

NASA Astrophysics Data System (ADS)

Mao, Mao; Lin, Jinru; Pan, Yuanming

2010-05-01

Hemimorphite is a refractory mineral in surface environments and occurs commonly in supergene non-sulfide Zn deposits and Zn mine tailings. Single-crystal electron paramagnetic resonance (EPR) spectra of gamma-ray-irradiated hemimorphite from Mapimi (Durango, Mexico) reveal two arsenic-associated oxyradicals: [AsO 4] 4- and [AsO 4] 2-. Inductively coupled plasma mass spectrometry analyses confirm this sample to contain 270 ppm As and that hemimorphite from other Zn deposits has appreciable amounts of arsenic as well. Spin Hamiltonian parameters, including matrices g, A ( 75As) and P( 75As), show that the [AsO 4] 4- radical formed from electron trapping by a locally uncompensated [AsO 4] 3- ion substituting for [SiO 4] 4-. Matrices g, A( 75As) and P( 75As) of the [AsO 4] 2- radical show it to have the unpaired spin on the bridging oxygen of an [AsO 4] 3- ion at a Si site and linked to a monovalent impurity ion. This structural model for the [AsO 4] 2- radical is further supported by observed 29Si and 1H superhyperfine structures arising from interactions with a single Si atom (A/g eβe = ˜1 mT at B// c) and two equivalent H atoms (A/g eβe = ˜0.3 mT at B∧ b = 10°), respectively. Hydrothermal experiments at 200 °C and ˜9.5 MPa show that hemimorphite contains up to ˜2.5 wt% As 2O 5 and suggest that both the arsenate concentration and the pH value in the solution affect the As content in hemimorphite. These results demonstrate that hemimorphite is capable of sequestering arsenate in its crystal lattice, hence is a natural sink for attenuating As in supergene non-sulfide Zn deposits and Zn mine tailings. Moreover, results from hemimorphite potentially have more far-reaching implications for major silicates such as zeolites in the immobilization and removal of arsenic in surface environments.

Developing a Carbon Observing System

NASA Astrophysics Data System (ADS)

Moore, B., III

2015-12-01

There is a clear need to better understand and predict future climate change, so that science can more confidently inform climate policy, including adaptation planning and future mitigation strategies. Understanding carbon cycle feedbacks, and the relationship between emissions (fossil and land use) and the resulting atmospheric carbon dioxide (CO2) and methane (CH4) concentrations in a changing climate has been recognized as an important goal by the IPCC. The existing surface greenhouse gas observing networks provide accurate and precise measurements of background values, but they are not configured to target the extended, complex and dynamic regions of the carbon budget. Space Agencies around the globe are committed to CO2 and CH4 observations: GOSAT-1/2, OCO-2/3, MERLin, TanSat, and CarbonSat. In addition to these Low Earth Orbit (LEO) missions, a new mission in Geostationary Orbit (GEO), geoCARB, which would provide mapping-like measurements of carbon dioxide, methane, and carbon monoxide concentrations over major land areas, has been recently proposed to the NASA Venture Program. These pioneering missions do not provide the spatial/temporal coverage to answer the key carbon-climate questions at process relevant scales nor do they address the distribution and quantification of anthropogenic sources at urban scales. They do demonstrate, however, that a well-planned future system of system integrating space-based LEO and GEO missions with extensive in situ observations could provide the accuracy, spatial resolution, and coverage needed to address critical open issues in the carbon-climate system. Dr. Diana Wickland devoted enormous energy in developing a comprehensive apprioach to understand the global carbon cycle; she understood well that an integrated, coordinated, international approach is needed. This shines through in her recent contribution in co-chairing the team that produced the "CEOS Strategy for Carbon Observations from Space." A NASA-funded community

Distributed snow data as a tool to inform water management decisions: Using Airborne Snow Observatory (ASO) at the Hetch Hetchy Reservoir in Yosemite National Park, City and County of San Francisco.

NASA Astrophysics Data System (ADS)

Graham, C. B.

2016-12-01

The timing and magnitude of spring snowmelt and runoff is critical in managing reservoirs in the Western United States. The Hetch Hetchy Reservoir in Yosemite National Park provides drinking water for 2.6 million customers in over 30 communities in the San Francisco Bay Area. Power generation from Hetch Hetchy meets the municipal load of the City and County of San Francisco. Water from the Hetch Hetchy Reservoir is also released in the Tuolumne River, supporting critical ecosystems in Yosemite National Park and the Stanislaus National Forest. Better predictions of long (seasonal) and short (weekly) term streamflow allow for more secure water resource planning, earlier power generation and ecologically beneficial releases from the Reservoir. Hetch Hetchy Reservoir is fed by snow dominated watersheds in the Sierra Mountains. Better knowledge of snowpack conditions allow for better predictions of inflows, both at the seasonal and at the weekly time scales. The ASO project has provided the managers of Hetch Hetchy Reservoir with high resolution estimates of total snowpack and snowpack distribution in the 460 mi2 Hetch Hetchy. We show that there is a tight correlation between snowpack estimates and future streamflow, allowing earlier, more confident operational decisions. We also show how distributed SWE estimates were used to develop and test a hydrologic model of the system (PRMS). This model, calibrated directly to snowpack conditions, is shown to correctly simulate snowpack volume and distribution, as well as streamflow patterns.

Characterizing long-term radon concentration changes in a geothermal area for correlation with volcanic earthquakes and reservoir temperatures: A case study from Mt. Aso, southwestern Japan

NASA Astrophysics Data System (ADS)

Koike, Katsuaki; Yoshinaga, Tohru; Asaue, Hisafumi

2014-04-01

The purpose of this study is to characterize in detail the temporal changes in Rn (radon-222) concentration in soil gases near fumaroles and clarify its correlation with volcanic earthquakes and temperatures in two geothermal reservoirs. Mt. Aso crater in southwest Japan, which has two reservoirs on its western side estimated by magnetotelluric survey to be at about 2 km in depth, was selected for this study. For the long-term survey, the α scintillation counter method was used weekly for 12.5 years at the three hot springs within a 2-km range. Rn concentrations were calculated using the CRAS method, a calculation method that considers radioactive equilibrium or nonequilibrium state of the soil gas. Rn concentrations generally showed similar fluctuation patterns among the sites. CRAS was used as a new indicator for evaluating the age of the soil gas. This age corresponds to the elapsed time determined from the generation of Rn based on the measurement of the numbers of atoms of Rn and its daughter 218Po at the start of measurement. In comparing the Rn data with the history of earthquakes in the Aso caldera, volcanic seismicity was identified as a major controlling factor in the sudden increase and decrease in Rn concentration as a function of age. For more precise detections of change, Rn concentrations were measured continuously at one site by pumping soil gas from a borehole and using an ionization chamber over 2.5 years. Five chemical components (He, H2, N2, CH4, and CO2) were then measured by gas chromatography at 1-week intervals. Because Rn concentrations are affected strongly by atmospheric temperatures, the residual components were obtained by subtracting the trend of the components from the original data. Chemical component data were used to estimate the temperature and pressure in the reservoir at the site; temperatures ranged from 229 to 280 °C, (average 265 °C, average pressure 80 MPa). Residual Rn concentrations showed a clear correlation with

Gulf of Mexico Coastal Ocean Observing System: The Gulf Component of the U.S. Integrated Ocean Observing System

NASA Astrophysics Data System (ADS)

Bernard, L. J.; Moersdorf, P. F.

2005-05-01

The United States is developing an Integrated Ocean Observing System (IOOS) as the U.S. component of the international Global Ocean Observing System (GOOS). IOOS consists of: (1) a coastal observing system for the U.S. EEZ, estuaries, and Great Lakes; and (2) a contribution to the global component of GOOS focused on climate and maritime services. The coastal component will consist of: (1) a National Backbone of observations and products from our coastal ocean supported by federal agencies; and (2) contributions of Regional Coastal Ocean Observing Systems (RCOOS). The Gulf of Mexico Coastal Ocean Observing System (GCOOS) is one of eleven RCOOS. This paper describes how GCOOS is progressing as a system of systems to carry out data collection, analysis, product generation, dissemination of information, and data archival. These elements are provided by federal, state, and local government agencies, academic institutions, non-government organization, and the private sector. This end-to-end system supports the seven societal goals of the IOOS, as provided by the U.S. Commission on Ocean Policy: detect and forecast oceanic components of climate variability, facilitate safe and efficient marine operations, ensure national security, manage marine resources, preserve and restore healthy marine ecosystems, mitigate natural hazards, and ensure public health. The initial building blocks for GCOOS include continuing in situ observations, satellite products, models, and other information supported by federal and state government, private industry, and academia. GCOOS has compiled an inventory of such activities, together with descriptions, costs, sources of support, and possible out-year budgets. These activities provide information that will have broader use as they are integrated and enhanced. GCOOS has begun that process by several approaches. First, GCOOS has established a web site (www.gcoos.org) which is a portal to such activities and contains pertinent information

Inhaled ENaC antisense oligonucleotide ameliorates cystic fibrosis-like lung disease in mice.

PubMed

Crosby, Jeff R; Zhao, Chenguang; Jiang, Chong; Bai, Dong; Katz, Melanie; Greenlee, Sarah; Kawabe, Hiroshi; McCaleb, Michael; Rotin, Daniela; Guo, Shuling; Monia, Brett P

2017-11-01

Epithelial sodium channel (ENaC, Scnn1) hyperactivity in the lung leads to airway surface dehydration and mucus accumulation in cystic fibrosis (CF) patients and in mice with CF-like lung disease. We identified several potent ENaC specific antisense oligonucleotides (ASOs) and tested them by inhalation in mouse models of CF-like lung disease. The inhaled ASOs distributed into lung airway epithelial cells and decreased ENaC expression by inducing RNase H1-dependent degradation of the targeted Scnn1a mRNA. Aerosol delivered ENaC ASO down-regulated mucus marker expression and ameliorated goblet cell metaplasia, inflammation, and airway hyper-responsiveness. Lack of systemic activity of ASOs delivered via the aerosol route ensures the safety of this approach. Our results demonstrate that antisense inhibition of ENaC in airway epithelial cells could be an effective and safe approach for the prevention and reversal of lung symptoms in CF and potentially other inflammatory diseases of the lung. Copyright © 2017 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

Chandra Observations of the Solar System

NASA Astrophysics Data System (ADS)

Lisse, Carey

2014-11-01

Many solar system objects are now known to emit X-rays due to charge-exchange between highly charged solar wind (SW) minor ions and neutrals in their extended atmospheres, including Earth, Venus, Mars, Jupiter, and the heliosphere, with total power outputs on the MW - GW scale. (Currently only upper limits exist for Saturn and Pluto.) Chandra observations of their morphology, spectra, and time dependence provide important information about the neutral atmosphere structure and the SW flux and charge state. Chandra observations of solar x-ray scattering from Earth, Venus, Mars, Jupiter, Saturn, and the Moon have also provided important clues for the scattering material and the solar radiation field at the body. We present here a 15 year summary of Chandra's solar system observations.

Computerized Observation System (COS) for Field Experiences.

ERIC Educational Resources Information Center

Reed, Thomas M.; And Others

The Computerized Observation System (COS) is a software program which an observer can use with a portable microcomputer to document preservice and inservice teacher performance. Specific observable behavior such as appropriate questions and responses shown to increase student achievement are recorded as Low Inference Observation Measures. Time on…

Asialoglycoprotein receptor 1 mediates productive uptake of N-acetylgalactosamine-conjugated and unconjugated phosphorothioate antisense oligonucleotides into liver hepatocytes

PubMed Central

Hettrick, Lisa; Revenko, Alexey; Kinberger, Garth A.; Prakash, Thazha P.; Seth, Punit P.

2017-01-01

Abstract Antisense oligonucleotide (ASO) therapeutics show tremendous promise for the treatment of previously intractable human diseases but to exert their effects on cellular RNA processing they must first cross the plasma membrane by endocytosis. The conjugation of ASOs to a receptor ligand can dramatically increase their entry into certain cells and tissues, as demonstrated by the implementation of N-acetylgalactosamine (GalNAc)-conjugated ASOs for Asialoglycoprotein Receptor (ASGR)-mediated uptake into liver hepatocytes. We compared the internalization and activity of GalNAc-conjugated ASOs and their parents in endogenous ASGR-expressing cells and were able to recapitulate hepatocyte ASO uptake and activity in cells engineered to heterologously express the receptor. We found that the minor receptor subunit, ASGR2, is not required for effective in vitro or in vivo uptake of GalNAc-conjugated ASO and that the major subunit, ASGR1, plays a small but significant role in the uptake of unconjugated phosphorothioate ASOs into hepatocytes. Moreover, our data demonstrates there is a large excess capacity of liver ASGR for the effective uptake of GalNAc–ASO conjugates, suggesting broad opportunities to exploit receptors with relatively moderate levels of expression. PMID:29069408

Earth Observing System, Conclusions and Recommendations

NASA Technical Reports Server (NTRS)

1984-01-01

The following Earth Observing Systems (E.O.S.) recommendations were suggested: (1) a program must be initiated to ensure that present time series of Earth science data are maintained and continued. (2) A data system that provides easy, integrated, and complete access to past, present, and future data must be developed as soon as possible. (3) A long term research effort must be sustained to study and understand these time series of Earth observations. (4) The E.O.S. should be established as an information system to carry out those aspects of the above recommendations which go beyond existing and currently planned activities. (5) The scientific direction of the E.O.S. should be established and continued through an international scientific steering committee.

Allele-Specific Inhibition of Rhodopsin With an Antisense Oligonucleotide Slows Photoreceptor Cell Degeneration

PubMed Central

Murray, Susan F.; Jazayeri, Ali; Matthes, Michael T.; Yasumura, Douglas; Yang, Haidong; Peralta, Raechel; Watt, Andy; Freier, Sue; Hung, Gene; Adamson, Peter S.; Guo, Shuling; Monia, Brett P.; LaVail, Matthew M.; McCaleb, Michael L.

2015-01-01

Purpose To preserve photoreceptor cell structure and function in a rodent model of retinitis pigmentosa with P23H rhodopsin by selective inhibition of the mutant rhodopsin allele using a second generation antisense oligonucleotide (ASO). Methods Wild-type mice and rats were treated with ASO by intravitreal (IVT) injection and rhodopsin mRNA and protein expression were measured. Transgenic rats expressing the murine P23H rhodopsin gene (P23H transgenic rat Line 1) were administered either a mouse-specific P23H ASO or a control ASO. The contralateral eye was injected with PBS and used as a comparator control. Electroretinography (ERG) measurements and analyses of the retinal outer nuclear layer were conducted and correlated with rhodopsin mRNA levels. Results Rhodopsin mRNA and protein expression was reduced after a single ASO injection in wild-type mice with a rhodopsin-specific ASO. Transgenic rat eyes that express a murine P23H rhodopsin gene injected with a murine P23H ASO had a 181 ± 39% better maximum amplitude response (scotopic a-wave) as compared with contralateral PBS-injected eyes; the response in control ASO eyes was not significantly different from comparator contralateral eyes. Morphometric analysis of the outer nuclear layer showed a significantly thicker nuclear layer in eyes injected with murine P23H ASO (18%) versus contralateral PBS-injected eyes. Conclusions Allele-specific ASO-mediated knockdown of mutant P23H rhodopsin expression slowed the rate of photoreceptor degeneration and preserved the function of photoreceptor cells in eyes of the P23H rhodopsin transgenic rat. Our data indicate that ASO treatment is a potentially effective therapy for the treatment of retinitis pigmentosa. PMID:26436889

An Observer's View of the ORAC System at UKIRT

NASA Astrophysics Data System (ADS)

Wright, G. S.; Bridger, A. B.; Pickup, D. A.; Tan, M.; Folger, M.; Economou, F.; Adamson, A. J.; Currie, M. J.; Rees, N. P.; Purves, M.; Kackley, R. D.

The Observatory Reduction and Acquisition Control system (ORAC) was commissioned with its first instrument at the UK Infrared Telescope (UKIRT) in October 1999, and with all of the other UKIRT instrumentation this year. ORAC's advance preparation Observing Tool makes it simpler to prepare and carry out observations. Its Observing Manager gives observers excellent feedback on their observing as it goes along, reducing wasted time. The ORAC pipelined Data Reduction system produces near-publication quality reduced data at the telescope. ORAC is now in use for all observing at UKIRT, including flexibly scheduled nights and service observing. This paper provides an observer's perspective of the system and its performance.

Synthesis and biological evaluation of sialyl-oligonucleotide conjugates targeting leukocyte B trans-membranal receptor CD22 as delivery agents for nucleic acid drugs.

PubMed

St-Pierre, Gabrielle; Pal, Sudip; Østergaard, Michael E; Zhou, Tianyuan; Yu, Jinghua; Tanowitz, Michael; Seth, Punit P; Hanessian, Stephen

2016-06-01

Antisense oligonucleotides (ASOs) modified with ligands which target cell surface receptors have the potential to significantly improve potency in the target tissue. This has recently been demonstrated using triantennary N-acetyl d-galactosamine conjugated ASOs. CD22 is a cell surface receptor expressed exclusively on B cells thus presenting an attractive target for B cell specific delivery of drugs. Herein, we reported the synthesis of monovalent and trivalent ASO conjugates with biphenylcarbonyl (BPC) modified sialic acids and their study as ASO delivery agents into B cells. CD22 positive cells exhibited reduced potency when treated with ligand modified ASOs and mechanistic examination suggested reduced uptake into cells potentially as a result of sequestration of ASO by other cell-surface proteins. Copyright © 2016 Elsevier Ltd. All rights reserved.

A Compute Perspective: Delivering Decision Support Products in 24 Hours from the Airborne Snow Observatory

NASA Astrophysics Data System (ADS)

Ramirez, P.; Mattmann, C. A.; Painter, T. H.; Seidel, F. C.; Trangsrud, A.; Hart, A. F.; Goodale, C. E.; Boardman, J. W.; Heneghan, C.; Verma, R.; Khudikyan, S.; Boustani, M.; Zimdars, P. A.; Horn, J.; Neely, S.

2013-12-01

The JPL Airborne Snow Observatory (ASO) must process 100s of GB of raw data to 100s of Terabytes of derived data in 24 hour Near Real Time (NRT) latency in a geographically distributed mobile compute and data-intensive processing setting. ASO provides meaningful information to water resource managers in the Western US letting them know how much water to maintain; or release, and what the prospectus of the current snow season is in the Sierra Nevadas. Providing decision support products processed from airborne data in a 24 hour timeframe is an emergent field and required the team to develop a novel solution as this process is typically done over months. We've constructed a system that combines Apache OODT; with Apache Tika; with the Interactive Data Analysis (IDL)/ENVI programming environment to rapidly and unobtrusively generate, distribute and archive ASO data as soon as the plane lands near Mammoth Lakes, CA. Our system is flexible, underwent several redeployments and reconfigurations, and delivered this critical information to stakeholders during the recent "Snow On" campaign March 2013 - June 2013. This talk will take you through a day in the life of the compute team from data acquisition, delivery, processing, and dissemination. Within this context, we will discuss the architecture of ASO; the open source software we used; the data we stored; and how it was delivered to its users. Moreover we will discuss the logistics, system engineering, and staffing that went into the developing, deployment, and operation of the mobile compute system.

NANOOS, the Northwest Association of Networked Ocean Observing Systems: a regional Integrated Ocean Observing System (IOOS) for the Pacific Northwest US

NASA Astrophysics Data System (ADS)

Newton, J.; Martin, D.; Kosro, M.

2012-12-01

NANOOS is the Northwest Association of Networked Ocean Observing Systems, the Pacific Northwest Regional Association of the United States Integrated Ocean Observing System (US IOOS). User driven since its inception in 2003, this regional observing system is responding to a variety of scientific and societal needs across its coastal ocean, estuaries, and shorelines. Regional priorities have been solicited and re-affirmed through active engagement with users and stakeholders. NANOOS membership is composed of an even mix of academic, governmental, industry, and non-profit organizations, who appoint representatives to the NANOOS Governing Council who confirm the priority applications of the observing system. NANOOS regional priorities are: Maritime Operations, Regional Fisheries, Ecosystem Assessment, Coastal Hazards, and Climate. NANOOS' regional coastal ocean observing system is implemented by seven partners (three universities, three state agencies, and one industry). Together, these partners conduct the observations, modeling, data management and communication, analysis products, education and outreach activities of NANOOS. Observations, designed to span coastal ocean, shorelines, and estuaries, include physical, chemical, biological and geological measurements. To date, modeling has been more limited in scope, but has provided the system with increased coverage for some parameters. The data management and communication system for NANOOS, led by the NANOOS Visualization System (NVS) is the cornerstone of the user interaction with NANOOS. NVS gives users access to observational data, both real time and archived, as well as modeling output. Given the diversity of user needs, measurements, and the complexity of the coastal environment, the challenge for the system is large. NANOOS' successes take advantage of technological advances, including real-time data transmission, profiling buoys, gliders, HF radars, and modeling. The most profound challenges NANOOS faces stem

VRP09 Reduction of Corneal Scarring Following Blast and Burn Injuries to Cornea Using siRNAs Targeting TGFb and CTGF

DTIC Science & Technology

2013-03-01

oligonucleotide-based drug approaches (better than ribozymes, antisense oligonucleotides ( ASO ), or microRNAs). (4) To accomplish these objectives, we...negative control scrambled ASO (designated NC). The combination of siRNAs T1 and R1 produced a knockdown of ~80% of TGFb1 protein in the conditioned...sequences (antisense oligonucleotides, ASOs ) into rabbit corneal cells and found that technique was very effective in delivering ASOs into the stroma and

Observing System Simulation Experiments for Fun and Profit

NASA Technical Reports Server (NTRS)

Prive, Nikki C.

2015-01-01

Observing System Simulation Experiments can be powerful tools for evaluating and exploring both the behavior of data assimilation systems and the potential impacts of future observing systems. With great power comes great responsibility - given a pure modeling framework, how can we be sure our results are meaningful? The challenges and pitfalls of OSSE calibration and validation will be addressed, as well as issues of incestuousness, selection of appropriate metrics, and experiment design. The use of idealized observational networks to investigate theoretical ideas in a fully complex modeling framework will also be discussed

Designing the Climate Observing System of the Future

NASA Astrophysics Data System (ADS)

Weatherhead, Elizabeth C.; Wielicki, Bruce A.; Ramaswamy, V.; Abbott, Mark; Ackerman, Thomas P.; Atlas, Robert; Brasseur, Guy; Bruhwiler, Lori; Busalacchi, Antonio J.; Butler, James H.; Clack, Christopher T. M.; Cooke, Roger; Cucurull, Lidia; Davis, Sean M.; English, Jason M.; Fahey, David W.; Fine, Steven S.; Lazo, Jeffrey K.; Liang, Shunlin; Loeb, Norman G.; Rignot, Eric; Soden, Brian; Stanitski, Diane; Stephens, Graeme; Tapley, Byron D.; Thompson, Anne M.; Trenberth, Kevin E.; Wuebbles, Donald

2018-01-01

Climate observations are needed to address a large range of important societal issues including sea level rise, droughts, floods, extreme heat events, food security, and freshwater availability in the coming decades. Past, targeted investments in specific climate questions have resulted in tremendous improvements in issues important to human health, security, and infrastructure. However, the current climate observing system was not planned in a comprehensive, focused manner required to adequately address the full range of climate needs. A potential approach to planning the observing system of the future is presented in this article. First, this article proposes that priority be given to the most critical needs as identified within the World Climate Research Program as Grand Challenges. These currently include seven important topics: melting ice and global consequences; clouds, circulation and climate sensitivity; carbon feedbacks in the climate system; understanding and predicting weather and climate extremes; water for the food baskets of the world; regional sea-level change and coastal impacts; and near-term climate prediction. For each Grand Challenge, observations are needed for long-term monitoring, process studies and forecasting capabilities. Second, objective evaluations of proposed observing systems, including satellites, ground-based and in situ observations as well as potentially new, unidentified observational approaches, can quantify the ability to address these climate priorities. And third, investments in effective climate observations will be economically important as they will offer a magnified return on investment that justifies a far greater development of observations to serve society's needs.

On the connection between autoimmunity, tic disorders and obsessive-compulsive disorders: a meta-analysis on anti-streptolysin O titres.

PubMed

Pozzi, Marco; Pellegrino, Paolo; Carnovale, Carla; Perrone, Valentina; Antoniazzi, Stefania; Perrotta, Cristiana; Radice, Sonia; Clementi, Emilio

2014-12-01

Anti-streptolysin O (ASO) titration is useful in the context of autoimmune pathologies, including specific cases of tic and obsessive-compulsive disorders occurring after streptococcal infections. There is currently a lack of consensus on the use of ASO titres; therefore we performed a meta-analysis to systematise available data and clarify the role of ASO titres in the context of neuropsychiatric disorders. A meta-analysis was performed on ASO titration in neuropsychiatric patients, including tic disorders and obsessive-compulsive disorders. Included studies reported numbers of positive subjects, depending on a chosen threshold, or detailed ASO titrations. Three hundred and twenty nine studies were identified, of which 13 were eligible for meta-analysis. Due to limited available data, only tic disorders were evaluated. The odds ratio of finding an abnormal ASO titre in patients was 3.22 (95% C.I. 1.51-6.88) as compared to healthy controls and 16.14 (95% C.I. 8.11-32.11) as compared to non-psychiatric patients. Studies using different thresholds were generally concordant. ASO titres were also compared quantitatively, finding an overall difference of the means of 70.50 U/ml (95% C.I. 25.21-115.80) in favour of patients with tic disorders. Based on current evidence, tic disorders are associated with a significant increase in ASO titres, evident both in a threshold-level perspective and on a quantitative level. These results encourage the systematisation of ASO titration in the context of tic disorders.

Discovery and Characterization of PRCAT47: A Novel Prostate Lineage and Cancer-Specific Long Noncoding RNA

DTIC Science & Technology

2017-07-01

targeting PRCAT47. ASOs were able to knock down PRCAT47 at high efficacy. Genes regulated upon ASO-mediated knockdown are highly correlated with...PRCAT47. ASOs were able to knock down PRCAT47 at high efficacy. Genes regulated upon ASO-mediated knockdown are highly correlated with that of siRNA...The following section will highlight the progress made in each sub-aims/ tasks proposed in the grant, including a detailed description of methods

The Global Ocean Observing System: One perspective

NASA Technical Reports Server (NTRS)

Wilson, J. Ron

1992-01-01

This document presents a possible organization for a Global Ocean Observing System (GOOS) within the Intergovernmental Oceanographic Commission and the joint ocean programs with the World Meteorological Organization. The document and the organization presented here is not intended to be definitive, complete or the best possible organization for such an observation program. It is presented at this time to demonstrate three points. The first point to be made is that an international program office for GOOS along the lines of the WOCE and TOGA IPOs is essential. The second point is that national programs will have to continue to collect data at the scale of WOCE plus TOGA and more. The third point is that there are many existing groups and committees within the IOC and joint IOC/WMO ocean programs that can contribute essential experience to and form part of the basis of a Global Ocean Observing System. It is particularly important to learn from what has worked and what has not worked in the past if a successful ocean observing system is to result.

Effective, Low-Cost Recovery of Toxic Arsenate Anions from Water by Using Hollow-Sphere Geode Traps.

PubMed

Shenashen, Mohamed A; Akhtar, Naeem; Selim, Mahmoud M; Morsy, Wafaa M; Yamaguchi, Hitoshi; Kawada, Satoshi; Alhamid, Abdulaziz A; Ohashi, Naoki; Ichinose, Izumi; Alamoudi, Ahmad S; El-Safty, Sherif A

2017-08-04

Because of the devastating impact of arsenic on terrestrial and aquatic organisms, the recovery, removal, disposal, and management of arsenic-contaminated water is a considerable challenge and has become an urgent necessity in the field of water treatment. This study reports the controlled fabrication of a low-cost adsorbent based on microscopic C-,N-doped NiO hollow spheres with geode shells composed of poly-CN nanospherical nodules (100 nm) that were intrinsically stacked and wrapped around the hollow spheres to form a shell with a thickness of 500-700 nm. This C-,N-doped NiO hollow-sphere adsorbent (termed CNN) with multiple diffusion routes through open pores and caves with connected open macro/meso windows over the entire surface and well-dispersed hollow-sphere particles that create vesicle traps for the capture, extraction, and separation of arsenate (AsO 4 3- ) species from aqueous solution. The CNN structures are considered to be a potentially attractive adsorbent for AsO 4 3- species due to 1) superior removal and trapping capacity from water samples and 2) selective trapping of AsO 4 3- from real water samples that mainly contained chloride and nitrate anions and Fe 2+ , and Mn 2+ , Ca 2+ , and Mg 2+ cations. The structural stability of the hierarchal geodes was evident after 20 cycles without any significant decrease in the recovery efficiency of AsO 4 3- species. To achieve low-cost adsorbents and toxic-waste management, this superior CNN AsO 4 3- dead-end trapping and recovery system evidently enabled the continuous control of AsO 4 3- disposal in water-scarce environments, presents a low-cost and eco-friendly adsorbent for AsO 4 3- species, and selectively produced water-free arsenate species. These CNN geode traps show potential as excellent adsorbent candidates in environment remediation tools and human healthcare. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comparison between anterior segmental osteotomy versus conventional orthodontic treatment in root resorption: a radiographic study using cone-beam computed tomography.

PubMed

Hwang, Bo-Yeon; Choi, Byung-Joon; Lee, Baek-Soo; Kwon, Yong-Dae; Lee, Jung-Woo; Jung, Junho; Ohe, Joo-Young

2017-12-01

Patients who received orthodontic treatment are likely to have apical root shortening. It appears that external apical root resorption results from a combination of patient-related risk factors such as genetic influences, systemic factors, and orthodontic treatment-related factors. Regarding the fact that the anterior segmental osteotomy (ASO) has been known for its possibility of complementing external apical root resorption and of buffering periodontal problems, it has been the preferred treatment. However, the studies on the efficacy of ASO in preserving the root are not sufficient. In this study, we compared the amount of root resorption between the patients who only received orthodontic treatment and the patients who received orthodontic treatment with ASO. This study included 28 patients (the number of incisor = 198) who received orthodontic treatment with or without ASO. We categorize them into groups A and B by the type of orthodontic treatment (group A: conventional orthodontic treatment; group B: orthodontic treatment with ASO). Cone-beam computed tomographic and cephalometric evaluations were retrospectively performed on the radiographs taken for the diagnosis of the treatment before treatment and at the end of active treatment. In group B, root resorption itself and its rate both turned out to have significantly lower than those in group A. Also, the change of incisal angle is significantly smaller in group B than in group A. On the other hand, in group A, the change of incisal angle was positively correlated with the change of AP (anteroposterior) position. In group B, the change of incisal angle was negatively correlated with the duration of the orthodontic treatment. In group B, amount of root resorption (mm) was positively correlated with the duration of the orthodontic treatment. The results show lesser root resorption and shorter treatment duration with ASO than with conventional orthodontic treatment. Therefore, if the indications are accurately

Semantics-enabled knowledge management for global Earth observation system of systems

NASA Astrophysics Data System (ADS)

King, Roger L.; Durbha, Surya S.; Younan, Nicolas H.

2007-10-01

The Global Earth Observation System of Systems (GEOSS) is a distributed system of systems built on current international cooperation efforts among existing Earth observing and processing systems. The goal is to formulate an end-to-end process that enables the collection and distribution of accurate, reliable Earth Observation data, information, products, and services to both suppliers and consumers worldwide. One of the critical components in the development of such systems is the ability to obtain seamless access of data across geopolitical boundaries. In order to gain support and willingness to participate by countries around the world in such an endeavor, it is necessary to devise mechanisms whereby the data and the intellectual capital is protected through procedures that implement the policies specific to a country. Earth Observations (EO) are obtained from a multitude of sources and requires coordination among different agencies and user groups to come to a shared understanding on a set of concepts involved in a domain. It is envisaged that the data and information in a GEOSS context will be unprecedented and the current data archiving and delivery methods need to be transformed into one that allows realization of seamless interoperability. Thus, EO data integration is dependent on the resolution of conflicts arising from a variety of areas. Modularization is inevitable in distributed environments to facilitate flexible and efficient reuse of existing ontologies. Therefore, we propose a framework for modular ontologies based knowledge management approach for GEOSS and present methods to enable efficient reasoning in such systems.

Classification framework for partially observed dynamical systems

NASA Astrophysics Data System (ADS)

Shen, Yuan; Tino, Peter; Tsaneva-Atanasova, Krasimira

2017-04-01

We present a general framework for classifying partially observed dynamical systems based on the idea of learning in the model space. In contrast to the existing approaches using point estimates of model parameters to represent individual data items, we employ posterior distributions over model parameters, thus taking into account in a principled manner the uncertainty due to both the generative (observational and/or dynamic noise) and observation (sampling in time) processes. We evaluate the framework on two test beds: a biological pathway model and a stochastic double-well system. Crucially, we show that the classification performance is not impaired when the model structure used for inferring posterior distributions is much more simple than the observation-generating model structure, provided the reduced-complexity inferential model structure captures the essential characteristics needed for the given classification task.

The Integrated Ocean Observing System Data Assembly Center

NASA Astrophysics Data System (ADS)

Bouchard, R. H.; Henderson, D.; Burnett, W.; Hervey, R. V.; Crout, R.

2008-05-01

The Integrated Ocean Observing System (IOOS) is the U.S. contribution to the Global Ocean Observing System and the Global Earth Observing System of Systems (GEOSS). As the Integrated Ocean Observing System Data Assembly Center (IOOS DAC), the National Oceanic and Atmospheric Administration`s (NOAA) National Data Buoy Center (NDBC) collects data from ocean observing systems and performs quality control on the data. Once the IOOS DAC performs the quality control, it distributes them in real-time: (1) in World Meteorological Organization alphanumeric data formats via the Global Telecommunications System (GTS) that provides instant availability to national and international users (2) in text files via its website (http://www.ndbc.noaa.gov) that provide easy access and use, and (3) in netCDF format via its OPeNDAP/DODS Server (http://dods.ndbc.noaa.gov) that provides higher resolution data than available in WMO alphanumeric or text file formats. The IOOS DAC routinely checks and distributes data from about 200 NDBC stations that include meteorological and oceanographic observations from buoys and coastal stations, water-level estimations from tsunameters (DART), and climate monitoring from buoys (Tropical Atmosphere Ocean array (TAO)). The IOOS DAC operates continuously - 24 hours per day, 7 days per week. In addition to data from NDBC`s platforms, the IOOS DAC applies its scientific expertise and data management and communications capabilities to facilitate partnerships for the exchange and application of data and to coordinate and leverage regional assets and resources from about 350 IOOS Partner stations. The IOOS DAC through its quality control process provides feedback to its partners on the quality of their observation that can lead to improved quality of the observations. The NDBC-IOOS Data Partnerships span the Western Hemisphere with data collection from the Beaufort Sea to the Peru Current, from the International Date Line to the central Atlantic Ocean, and

Observations and Numerical Modelling of Strong Meteotsunami of 13 June 2013 on the East Coast of the USA

NASA Astrophysics Data System (ADS)

Fine, I.; Sepic, J.; Rabinovich, A.; Thomson, R.

2014-12-01

A strong "derecho" (rapidly moving lines of convectively induced intense thunderstorms) was generated over the Midwestern United States on 12-13 June 2013 and propagated across the Appalachian Mountains to the Atlantic Ocean. Three hours after the derecho crossed the Atlantic coast, a ~2-m high meteotsunami wave was reported to have hit the New Jersey coast. Significant tsunami-like oscillations, with wave heights of ~0.6 m, were also recorded by a number of tide-gauges located along the eastern seaboard from Nova Scotia to South Carolina, at Bermuda, and by open-ocean DART 44402. These observations triggered the tsunami-alert mode of the DART station. Intense air pressure disturbances (with pressure change of 3-6 hPa in 20 min) and strong winds were observed at a number of National Oceanic and Atmospheric Administration (NOAA) and Automated Surface Observing System (ASOS) stations to be propagating simultaneously with the derecho system, indicating that the pressure disturbances were the primary cause for the sea level oscillations in Chesapeake and Delaware bays. The air pressure disturbance continued to propagate seaward over the continental shelf, thereby generating long waves via Proudman resonance at those areas of the shelf where the propagation speed of the air pressure disturbance matched the long wave speed. Upon reaching the shelf break, the long-waves were partly transmitted (reaching Bermuda 5 hours later) and partly reflected (returning to the east coast of the US and Canada 3 to 6 hours later). A numerical barotropic ocean model forced with idealized air pressure and wind fields was used successfully to simulate the event. The meteotsunami arrival times and maximum wave heights obtained from the model closely match the measured values and confirm initial assumptions regarding the partitioning between transmitted and reflected meteotsunami waves.

Interoperable Access to Near Real Time Ocean Observations with the Observing System Monitoring Center

NASA Astrophysics Data System (ADS)

O'Brien, K.; Hankin, S.; Mendelssohn, R.; Simons, R.; Smith, B.; Kern, K. J.

2013-12-01

The Observing System Monitoring Center (OSMC), a project funded by the National Oceanic and Atmospheric Administration's Climate Observations Division (COD), exists to join the discrete 'networks' of In Situ ocean observing platforms -- ships, surface floats, profiling floats, tide gauges, etc. - into a single, integrated system. The OSMC is addressing this goal through capabilities in three areas focusing on the needs of specific user groups: 1) it provides real time monitoring of the integrated observing system assets to assist management in optimizing the cost-effectiveness of the system for the assessment of climate variables; 2) it makes the stream of real time data coming from the observing system available to scientific end users into an easy-to-use form; and 3) in the future, it will unify the delayed-mode data from platform-focused data assembly centers into a standards- based distributed system that is readily accessible to interested users from the science and education communities. In this presentation, we will be focusing on the efforts of the OSMC to provide interoperable access to the near real time data stream that is available via the Global Telecommunications System (GTS). This is a very rich data source, and includes data from nearly all of the oceanographic platforms that are actively observing. We will discuss how the data is being served out using a number of widely used 'web services' (including OPeNDAP and SOS) and downloadable file formats (KML, csv, xls, netCDF), so that it can be accessed in web browsers and popular desktop analysis tools. We will also be discussing our use of the Environmental Research Division's Data Access Program (ERDDAP), available from NOAA/NMFS, which has allowed us to achieve our goals of serving the near real time data. From an interoperability perspective, it's important to note that access to the this stream of data is not just for humans, but also for machine-to-machine requests. We'll also delve into how we

76 FR 19746 - Approval for Extension of Subzone Status and Manufacturing Authority; Foreign-Trade Subzone 169A...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-08

... Subzone Status and Manufacturing Authority; Foreign-Trade Subzone 169A; Aso LLC (Adhesive Bandages... indefinitely extend subzone status and manufacturing authority on behalf of Aso LLC (Aso) to perform adhesive... and manufacturing authority for the production of adhesive bandages under zone procedures within...

RNA helicase A is not required for RISC activity.

PubMed

Liang, Xue-Hai; Crooke, Stanley T

2013-10-01

It has been shown that siRNAs can compete with each other or with endogenous miRNAs for RISC components. This competition may complicate the interpretations of phenotypes observed through siRNA-mediated knockdown of genes, especially those genes implicated in the RISC pathway. In this study, we re-examined the function of RNA helicase A (RHA), which has been previously proposed to function in RISC loading based on siRNA-mediated knockdown studies. Here we show that reduced RISC activity or loading of siRNAs was observed only in cells depleted of RHA using siRNA, but not using RNaseH-dependent antisense oligonucleotides (ASOs), suggesting that the impaired RISC function stems from the competition between pre-existing and newly transfected siRNAs, but not from reduction of the RHA protein. This view is further supported by the findings that cells depleted of a control protein, NCL1, using siRNA, but not ASO, exhibited similar defects on the loading and activity of a subsequently transfected siRNA. Transfection of RHA or NCL1 siRNAs, but not ASOs, reduced the levels of endogenous miRNAs, suggesting a competition mechanism. As a positive control, we showed that reduction of MOV10 by either siRNA or ASO decreased siRNA activity, confirming its role in RISC function. Together, our results indicate that RHA is not required for RISC activity or loading, and suggest that proper controls are required when using siRNAs to functionalize genes to avoid competition effects. © 2013. Published by Elsevier B.V. All rights reserved.

Optical data communication for Earth observation satellite systems

NASA Astrophysics Data System (ADS)

Fischer, J.; Loecherbach, E.

1991-10-01

The current development status of optical communication engineering in comparison to the conventional microwave systems and the different configurations of the optical data communication for Earth observation satellite systems are described. An outlook to future optical communication satellite systems is given. During the last decade Earth observation became more and more important for the extension of the knowledge about our planet and the human influence on nature. Today pictures taken by satellites are used, for example, to discover mineral resources or to predict harvest, crops, climate, and environment variations and their influence on the population. A new and up to date application for Earth observation satellites can be the verification of disarmament arrangements and the control of crises areas. To solve these tasks a system of Earth observing satellites with sensors tailored to the envisaged mission is necessary. Besides these low Earth orbiting satellites, a global Earth observation system consists of at least two data relay satellites. The communication between the satellites will be established via Inter-Satellite Links (ISL) and Inter-Orbit Links (IOL). On these links, bitrates up to 1 Gbit/s must be taken into account. Due to the increasing scarcity of suitable frequencies, higher carrier frequencies must probably be considered, and possible interference with terrestrial radio relay systems are two main problems for a realization in microwave technique. One important step to tackle these problems is the use of optical frequencies for IOL's and ISL's.

Microscopic mechanism of amino silicone oil modification and modification effect with different amino group contents based on molecular dynamics simulation

NASA Astrophysics Data System (ADS)

He, Liping; Li, Wenjun; Chen, Dachuan; Yuan, Jianmin; Lu, Gang; Zhou, Dianwu

2018-05-01

The microscopic mechanism of amino silicone oil (ASO) modification of natural fiber was investigated for the first time using molecular dynamics (MD) simulation at the atomic and molecular levels. The MD simulation results indicated that the ASO molecular interacted with the cellulose molecular within the natural fiber, mainly by intermolecular forces of Nsbnd Hsbnd O and Osbnd Hsbnd N hydrogen bonds and the molecular chain of ASO absorbed onto the natural fiber in a selective orientation, i.e., the hydrophobic alkyl groups (sbnd CnH2n+1) project outward and the polar amino groups (sbnd NH2) point to the surface of natural fiber. Consequently, the ASO modification changed the surface characteristic of natural fiber from hydrophilic to hydrophobic. Furthermore, the modification effects of the ASO modification layer with different amino group contents (m:n ratio) were also evaluated in this study by calculating the binding energy between the ASO modifier and natural fiber, and the cohesive energy density and free volume of the ASO modification layer. The results showed that the binding energy reached a maximum when the m:n ratio of ASO was of 8:4, suggesting that a good bonding strength was achieved at this m:n ratio. It was also found that the cohesive energy density enhanced with the increase in the amino group content, and the higher the cohesive energy density, the easier the formation of the ASO modification layer. However, the fraction free volume decreased with the increase in the amino group content. This is good for improving the water-proof property of natural fiber. The present work can provide an effective method for predicting the modification effects and designing the optimized m:n ratio of ASO modification.

Predictive dose-based estimation of systemic exposure multiples in mouse and monkey relative to human for antisense oligonucleotides with 2'-o-(2-methoxyethyl) modifications.

PubMed

Yu, Rosie Z; Grundy, John S; Henry, Scott P; Kim, Tae-Won; Norris, Daniel A; Burkey, Jennifer; Wang, Yanfeng; Vick, Andrew; Geary, Richard S

2015-01-20

Evaluation of species differences and systemic exposure multiples (or ratios) in toxicological animal species versus human is an ongoing exercise during the course of drug development. The systemic exposure ratios are best estimated by directly comparing area under the plasma concentration-time curves (AUCs), and sometimes by comparing the dose administered, with the dose being adjusted either by body surface area (BSA) or body weight (BW). In this study, the association between AUC ratio and the administered dose ratio from animals to human were studied using a retrospective data-driven approach. The dataset included nine antisense oligonucleotides (ASOs) with 2'-O-(2-methoxyethyl) modifications, evaluated in two animal species (mouse and monkey) following single and repeated parenteral administrations. We found that plasma AUCs were similar between ASOs within the same species, and are predictable to human exposure using a single animal species, either mouse or monkey. Between monkey and human, the plasma exposure ratio can be predicted directly based on BW-adjusted dose ratios, whereas between mouse and human, the exposure ratio would be nearly fivefold lower in mouse compared to human based on BW-adjusted dose values. Thus, multiplying a factor of 5 for the mouse BW-adjusted dose would likely provide a reasonable AUC exposure estimate in human at steady-state.

Planning for the Global Earth Observation System of Systems (GEOSS)

USGS Publications Warehouse

Christian, E.

2005-01-01

The Group on Earth Observations was established to promote comprehensive, coordinated, and sustained Earth observations. Its mandate is to implement the Global Earth Observation System of Systems (GEOSS) in accord with the GEOSS 10-Year Implementation Plan and Reference Document. During the months over which the GEOSS Implementation Plan was developed, many issues surfaced and were addressed. This article discusses several of the more interesting or challenging of those issues-e.g. fitting in with existing organizations and securing stable funding - some of which have yet to be resolved fully as of this writing. Despite the relatively short period over which the Implementation Plan had to be developed, there is a good chance that the work undertaken will be influential for decades to come. ?? 2005 Elsevier Ltd. All rights reserved.

Ab initio calculation of harmonic force fields and vibrational spectra for the arsine oxides and sulfides R sub 3 AsY (R = H, F; Y = O, S) and related compounds

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schneider, W.; Thiel, W.; Komornicki, A.

1990-04-05

Ab initio self-consistent-field calculations using effective core potentials and polarized double-zeta basis sets are reported for the arsenic compounds H{sub 3}As, H{sub 3}AsO, H{sub 3}AsS, F{sub 3}As, F{sub 3}AsO, F{sub 3}AsS, cis- and trans-H{sub 2}AsOH, and HAsO. The calculated geometries, rotational constants, vibrational frequencies, Coriolis coupling constants, centrifugal distortion constants, infrared band intensities, and force fields are compared with the available experimental data. Good agreement is found in the case of the known molecules, especially H{sub 3}As and F{sub 3}As, so that the predictions for the unknown molecules are expected to be realistic. The theoretical results confirm a recent spectroscopicmore » identification of H{sub 3}AsO, H{sub 2}AsOH, and HAsO and suggest reassignment of several observed frequencies.« less

Team Formation in Partially Observable Multi-Agent Systems

NASA Technical Reports Server (NTRS)

Agogino, Adrian K.; Tumer, Kagan

2004-01-01

Sets of multi-agent teams often need to maximize a global utility rating the performance of the entire system where a team cannot fully observe other teams agents. Such limited observability hinders team-members trying to pursue their team utilities to take actions that also help maximize the global utility. In this article, we show how team utilities can be used in partially observable systems. Furthermore, we show how team sizes can be manipulated to provide the best compromise between having easy to learn team utilities and having them aligned with the global utility, The results show that optimally sized teams in a partially observable environments outperform one team in a fully observable environment, by up to 30%.

Construction of a patient observation system using KINECTTM

NASA Astrophysics Data System (ADS)

Miyaura, Kazunori; Kumazaki, Yu; Fukushima, Chika; Kato, Shingo; Saitoh, Hidetoshi

2014-03-01

Improvement in the positional accuracy of irradiation is expected by capturing patient motion (intra-fractional error) during irradiation. The present study reports the construction of a patient observation system using Microsoft® KINECTTM. By tracking movement, we made it possible to add a depth component to the acquired position coordinates and to display three-axis (X, Y, and Z) movement. Moreover, the developed system can be displayed in a graph which is constructed from the coordinate position at each time interval. Using the developed system, an observer can easily visualize patient movement. When the body phantom was moved a known distance in the X, Y, and Z directions, good coincidence was shown with each axis. We built a patient observation system which captures a patient's motion using KINECTTM.

An Introduction to Observing System Simulation Experiments

NASA Technical Reports Server (NTRS)

Prive, Nikki C.

2017-01-01

Observing System Simulation Experiments (OSSEs) are used to estimate the potential impact of proposed new instruments and data on numerical weather prediction. OSSEs can also be used to help design new observing platforms and to investigate the behavior of data assimilation systems. A basic overview of how to design and perform an OSSE will be given, as well as best practices and pitfalls. Some examples using the OSSE framework developed at the NASA Global Modeling and Assimilation Office will be shown.

76 FR 21703 - Approval for Extension of Subzone Status and Manufacturing Authority; Foreign-Trade Subzone 169A...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-18

... Subzone Status and Manufacturing Authority; Foreign-Trade Subzone 169A; Aso LLC; (Adhesive Bandages... indefinitely extend subzone status and manufacturing authority on behalf of Aso LLC, to perform adhesive... LLC (Aso) to perform adhesive bandage manufacturing within FTZ Subzone 169A in Sarasota County...

Intra-caldera active fault: An example from the Mw 7.0 2016 Kumamoto, Japan, earthquake

NASA Astrophysics Data System (ADS)

Toda, S.; Murakami, T.; Takahashi, N.

2017-12-01

A NE-trending 30-km-long surface rupture with up to 2.4 m dextral slip emerged during the Mw=7.0 16 April 2016 Kumamoto earthquake along the previously mapped Futagawa and northern Hinagu fault systems. The 5-km-long portion of the northeast rupture end, which was previously unidentified, crossed somma and extended to the 20-km-diameter Aso Caldera, one of the major active volcanoes, central Kyushu. We here explore geologic exposures of interplays of active faulting and active volcanism, and then argue the Futagawa fault system has been influenced by the ring fault system associated with the caldera forming gigantic eruptions since 270 ka, last of which occurred 90 ka ejecting a huge amount of ignimbrite. To understand the interplays, together with the mapping of the 2016 rupture, we employed an UAV to capture numerous photos of the exposures along the canyon and developed 3D orthochromatic topographic model using PhotoScan. One-hundred-meter-deep Kurokawa River canyon by the Aso Caldera rim exposes two lava flow units of 50 ka vertically offset by 10 m by the Futatawa fault system. Reconstructions of the collapsed bridges across the Kurokawa River also reveal cross sections of a 30-meter-high tectonic bulge and 10-m-scale negative flower structure deformed by the frequent fault movements. We speculate two fault developing models across the Aso Caldera. One is that the NE edge of the Futagawa fault system was cut and reset by the caldera forming ring fault, which indicates the 3-km-long rupture extent within the Aso Caldera would be a product of the fault growth since the last Aso-4 eruption of 90 ka. It enables us to estimate the 33 mm/yr of the fault propagation speed. An alternative model is that subsurface rupture of the Kumamoto earthquake extended further to the NE rim, the other side of the caldera edge, which is partially supported by the geodetic and seismic inversions. With respect to the model, the clear surface rupture of the 2016 Kumamoto earthquake

Earth Observing System Covariance Realism Updates

NASA Technical Reports Server (NTRS)

Ojeda Romero, Juan A.; Miguel, Fred

2017-01-01

This presentation will be given at the International Earth Science Constellation Mission Operations Working Group meetings June 13-15, 2017 to discuss the Earth Observing System Covariance Realism updates.

An Observation-based Assessment of Instrument Requirements for a Future Precipitation Process Observing System

NASA Astrophysics Data System (ADS)

Nelson, E.; L'Ecuyer, T. S.; Wood, N.; Smalley, M.; Kulie, M.; Hahn, W.

2017-12-01

Global models exhibit substantial biases in the frequency, intensity, duration, and spatial scales of precipitation systems. Much of this uncertainty stems from an inadequate representation of the processes by which water is cycled between the surface and atmosphere and, in particular, those that govern the formation and maintenance of cloud systems and their propensity to form the precipitation. Progress toward improving precipitation process models requires observing systems capable of quantifying the coupling between the ice content, vertical mass fluxes, and precipitation yield of precipitating cloud systems. Spaceborne multi-frequency, Doppler radar offers a unique opportunity to address this need but the effectiveness of such a mission is heavily dependent on its ability to actually observe the processes of interest in the widest possible range of systems. Planning for a next generation precipitation process observing system should, therefore, start with a fundamental evaluation of the trade-offs between sensitivity, resolution, sampling, cost, and the overall potential scientific yield of the mission. Here we provide an initial assessment of the scientific and economic trade-space by evaluating hypothetical spaceborne multi-frequency radars using a combination of current real-world and model-derived synthetic observations. Specifically, we alter the field of view, vertical resolution, and sensitivity of a hypothetical Ka- and W-band radar system and propagate those changes through precipitation detection and intensity retrievals. The results suggest that sampling biases introduced by reducing sensitivity disproportionately affect the light rainfall and frozen precipitation regimes that are critical for warm cloud feedbacks and ice sheet mass balance, respectively. Coarser spatial resolution observations introduce regime-dependent biases in both precipitation occurrence and intensity that depend on cloud regime, with even the sign of the bias varying within a

Susquehanna River Basin Hydrologic Observing System (SRBHOS)

NASA Astrophysics Data System (ADS)

Reed, P. M.; Duffy, C. J.; Dressler, K. A.

2004-12-01

In response to the NSF-CUAHSI initiative for a national network of Hydrologic Observatories, we propose to initiate the Susquehanna River Basin Hydrologic Observing System (SRBHOS), as the northeast node. The Susquehanna has a drainage area of 71, 410 km2. From the headwaters near Cooperstown, NY, the river is formed within the glaciated Appalachian Plateau physiographic province, crossing the Valley and Ridge, then the Piedmont, before finishing its' 444 mile journey in the Coastal Plain of the Chesapeake Bay. The Susquehanna is the major source of water and nutrients to the Chesapeake. It has a rich history in resource development (logging, mining, coal, agriculture, urban and heavy industry), with an unusual resilience to environmental degradation, which continues today. The shallow Susquehanna is one of the most flood-ravaged rivers in the US with a decadal regularity of major damage from hurricane floods and rain-on-snow events. As a result of this history, it has an enormous infrastructure for climate, surface water and groundwater monitoring already in place, including the nations only regional groundwater monitoring system for drought detection. Thirty-six research institutions have formed the SRBHOS partnership to collaborate on a basin-wide network design for a new scientific observing system. Researchers at the partner universities have conducted major NSF research projects within the basin, setting the stage and showing the need for a new terrestrial hydrologic observing system. The ultimate goal of SRBHOS is to close water, energy and solute budgets from the boundary layer to the water table, extending across plot, hillslope, watershed, and river basin scales. SRBHOS is organized around an existing network of testbeds (legacy watershed sites) run by the partner universities, and research institutions. The design of the observing system, when complete, will address fundamental science questions within major physiographic regions of the basin. A nested

Outcomes after corrective surgery for congenital dextro-transposition of the great arteries using the arterial switch technique: a protocol for a scoping systematic review

PubMed Central

Mbuagbaw, Lawrence; Forlemu-Kamwa, Doris; Chu, Angela; Thabane, Lehana; Dillenberg, Rejane

2014-01-01

Introduction Dextro-transposition of the great arteries (d-TGA) is a life-threatening congenital health defect that requires rapid surgery. The most widely used approach is the arterial switch operation (ASO) developed by Jatene in the 1970s. The first set of children who received this intervention are now adults. The objective of this scoping review of the literature was to document the short-term (less than 1 year), medium-term (1–20 years) and long-term (more than 20 years) outcomes in children who underwent the ASO. Our primary income is survival, but we will explore other secondary surgical, cardiovascular, reproductive and quality-of-life outcomes. Methods and analyses Using a systematic scoping review approach, we will conduct a systematic search of the published literature for experimental and observational studies published on children who received the ASO intervention for classic d-TGA. We will search MEDLINE, Excerpta Medica Database (EMBASE), Cumulative Index to Nursing and Allied Health Literature (CINAHL) and Literatura Latino Americana em Ciências da Saúde (LILACS) from 1973 (date of the first successful ASO) to February 2014. Identified articles will be screened in duplicate and full text for selected articles will be retrieved. Data extraction will be carried out in duplicate. Discrepancies will be resolved by consensus or by consulting a third author. Where possible, proportions will be pooled using the inverse variance method. Our findings will be reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and Meta-analysis Of Observational studies in Epidemiology (MOOSE) guidelines. Ethics and dissemination The results of this paper will be disseminated as peer-reviewed publications, at conferences and at clinical rounds. Our findings may answer important questions for surgeons who perform the ASO intervention and for clinicians who take care of patients after surgery and throughout their

Structure and spectroscopic study of aqueous Fe(III)-As(V) complexes using UV-Vis, XAS and DFT-TDDFT.

PubMed

Chai, Liyuan; Yang, Jinqin; Zhang, Ning; Wu, Pin-Jiun; Li, Qingzhu; Wang, Qingwei; Liu, Hui; Yi, Haibo

2017-09-01

Aqueous complexes between ferric (Fe(III)) and arsenate (As(V)) are indispensable for understanding the mobility of arsenic (As) in Fe(III)-As(V)-rich systems. In this study, aqueous Fe(III)-As(V) complexes, FeH 2 AsO 4 2+ and FeHAsO 4 + , were postulated based on the qualitative analysis of UV-Vis spectra in both Fe(III)-As(V)-HClO 4 and Fe(III)-As(V)-H 2 SO 4 systems. Subsequently, monodentate structures were evidenced by Fe K-edge EXAFS and modeled as [FeH 2 AsO 4 (H 2 O) 5 ] 2+ and [FeHAsO 4 (H 2 O) 5 ] + by DFT. The feature band at ∼280 nm was verified as electron excitation chiefly from Fe-As-bridged O atoms to d-orbital of Fe in [FeH 2 AsO 4 (H 2 O) 5 ] 2+ and [FeHAsO 4 (H 2 O) 5 ] + . The structural and spectral information of Fe(III)-As(V) complexes will enable future speciation analysis in Fe(III)-As(V)-rich system. Copyright © 2017. Published by Elsevier Ltd.

NASA's mission to planet Earth: Earth observing system

NASA Technical Reports Server (NTRS)

1993-01-01

The topics covered include the following: global climate change; radiation, clouds, and atmospheric water; the ocean; the troposphere - greenhouse gases; land cover and the water cycle; polar ice sheets and sea level; the stratosphere - ozone chemistry; volcanoes; the Earth Observing System (EOS) - how NASA will support studies of global climate change?; research and assessment - EOS Science Investigations; EOS Data and Information System (EOSDIS); EOS observations - instruments and spacecraft; a national international effort; and understanding the Earth System.

Control of phosphorothioate stereochemistry substantially increases the efficacy of antisense oligonucleotides.

PubMed

Iwamoto, Naoki; Butler, David C D; Svrzikapa, Nenad; Mohapatra, Susovan; Zlatev, Ivan; Sah, Dinah W Y; Meena; Standley, Stephany M; Lu, Genliang; Apponi, Luciano H; Frank-Kamenetsky, Maria; Zhang, Jason Jingxin; Vargeese, Chandra; Verdine, Gregory L

2017-09-01

Whereas stereochemical purity in drugs has become the standard for small molecules, stereoisomeric mixtures containing as many as a half million components persist in antisense oligonucleotide (ASO) therapeutics because it has been feasible neither to separate the individual stereoisomers, nor to synthesize stereochemically pure ASOs. Here we report the development of a scalable synthetic process that yields therapeutic ASOs having high stereochemical and chemical purity. Using this method, we synthesized rationally designed stereopure components of mipomersen, a drug comprising 524,288 stereoisomers. We demonstrate that phosphorothioate (PS) stereochemistry substantially affects the pharmacologic properties of ASOs. We report that Sp-configured PS linkages are stabilized relative to Rp, providing stereochemical protection from pharmacologic inactivation of the drug. Further, we elucidated a triplet stereochemical code in the stereopure ASOs, 3'-SpSpRp, that promotes target RNA cleavage by RNase H1 in vitro and provides a more durable response in mice than stereorandom ASOs.

Promoting discovery and access to real time observations produced by regional coastal ocean observing systems

NASA Astrophysics Data System (ADS)

Anderson, D. M.; Snowden, D. P.; Bochenek, R.; Bickel, A.

2015-12-01

In the U.S. coastal waters, a network of eleven regional coastal ocean observing systems support real-time coastal and ocean observing. The platforms supported and variables acquired are diverse, ranging from current sensing high frequency (HF) radar to autonomous gliders. The system incorporates data produced by other networks and experimental systems, further increasing the breadth of the collection. Strategies promoted by the U.S. Integrated Ocean Observing System (IOOS) ensure these data are not lost at sea. Every data set deserves a description. ISO and FGDC compliant metadata enables catalog interoperability and record-sharing. Extensive use of netCDF with the Climate and Forecast convention (identifying both metadata and a structured format) is shown to be a powerful strategy to promote discovery, interoperability, and re-use of the data. To integrate specialized data which are often obscure, quality control protocols are being developed to homogenize the QC and make these data more integrate-able. Data Assembly Centers have been established to integrate some specialized streams including gliders, animal telemetry, and HF radar. Subsets of data that are ingested into the National Data Buoy Center are also routed to the Global Telecommunications System (GTS) of the World Meteorological Organization to assure wide international distribution. From the GTS, data are assimilated into now-cast and forecast models, fed to other observing systems, and used to support observation-based decision making such as forecasts, warnings, and alerts. For a few years apps were a popular way to deliver these real-time data streams to phones and tablets. Responsive and adaptive web sites are an emerging flexible strategy to provide access to the regional coastal ocean observations.

Observing Natural Hazards: Tsunami, Hurricane, and El Niño Observations from the NDBC Ocean Observing System of Systems

NASA Astrophysics Data System (ADS)

O'Neil, K.; Bouchard, R.; Burnett, W. H.; Aldrich, C.

2009-12-01

The National Oceanic and Atmospheric Administration’s (NOAA) National Data Buoy Center (NDBC) operates and maintains the NDBC Ocean Observing Systems of Systems (NOOSS), comprised of 3 networks that provide critical information before and during and after extreme hazards events, such as tsunamis, hurricanes, and El Niños. While each system has its own mission, they have in common the requirement to remain on station in remote areas of the ocean to provide reliable and accurate observations. After the 2004 Sumatran Tsunami, NOAA expanded its network of tsunameters from six in the Pacific Ocean to a vast network of 39 stations providing information to Tsunami Warning Centers to enable faster and more accurate tsunami warnings for coastal communities in the Pacific, Atlantic, Caribbean and the Gulf of Mexico. The tsunameter measurements are used to detect the amplitude and period of the tsunamis, and the data can be assimilated into models for the prediction and impact of the tsunamis to coastal communities. The network has been used for the detection of tsunamis generated by earthquakes, including the 2006 and 2007 Kuril Islands, 2007 Peru, and Solomon Islands, and most recently for the 2009 Dusky Sound, New Zealand earthquake. In August 2009, the NOAA adjusted its 2009 Atlantic Hurricane Seasonal Outlooks from above normal to near or below normal activity, primarily due to a strengthening El Niño. A key component in the detection of that El Niño was the Tropical Atmosphere Ocean Array (TAO) operated by NDBC. TAO provides real-time data for improved detection, understanding, and prediction of El Niño and La Niña. The 55-buoy TAO array spans the central and eastern equatorial Pacific providing real-time and post-deployment recovery data to support climate analysis and forecasts. Although, in this case, the El Niño benefits the tropical Atlantic, the alternate manifestation, La Niña typically enhances hurricane activity in the Atlantic. The various phases of

Exploring our outer solar system - The Giant Planet System Observers

NASA Astrophysics Data System (ADS)

Cooper, J. F.; Sittler, E. C., Jr.; Sturner, S. J.; Pitman, J. T.

As space-faring peoples now work together to plan and implement future missions that robotically prepare for landing humans to explore the Moon, and later Mars, the time is right to develop evolutionary approaches for extending this next generation of exploration beyond Earth's terrestrial planet neighbors to the realm of the giant planets. And while initial fly-by missions have been hugely successful in providing exploratory surveys of what lies beyond Mars, we need to consider now what robotic precursor mission capabilities we need to emplace that prepare us properly, and comprehensively, for long-term robotic exploration, and eventual human habitation, beyond Mars to the outer reaches of our solar system. To develop practical strategies that can establish prioritized capabilities, and then develop a means for achieving those capabilities within realistic budget and technology considerations, and in reasonable timeframes, is our challenge. We suggest one component of such an approach to future outer planets exploration is a series of Giant Planets System Observer (GPSO) missions that provide for long- duration observations, monitoring, and relay functions to help advance our understanding of the outer planets and thereby enable a sound basis for planning their eventual exploration by humans. We envision these missions as being comparable to taking Hubble-class remote-sensing facilities, along with the space physics capabilities of long-lived geospace and heliospheric missions, to the giant planet systems and dedicating long observing lifetimes (HST, 16 yr.; Voyagers, 29 yr.) to the exhaustive study and characterization of those systems. GPSO missions could feature 20-yr+ extended mission lifetimes, direct inject trajectories to maximize useful lifetime on target, placement strategies that take advantage of natural environment shielding (e.g., Ganymede magnetic field) where possible, orbit designs having favorable planetary system viewing geometries, comprehensive

Spanish Earth Observation Satellite System

NASA Astrophysics Data System (ADS)

Borges, A.; Cerezo, F.; Fernandez, M.; Lomba, J.; Lopez, M.; Moreno, J.; Neira, A.; Quintana, C.; Torres, J.; Trigo, R.; Urena, J.; Vega, E.; Vez, E.

2010-12-01

The Spanish Ministry of Industry, Tourism and Trade (MITyC) and the Ministry of Defense (MoD) signed an agreement in 2007 for the development of a "Spanish Earth Observation Satellite System" based, in first instance, on two satellites: a high resolution optical satellite, called SEOSAT/Ingenio, and a radar satellite based on SAR technology, called SEOSAR/Paz. SEOSAT/Ingenio is managed by MITyC through the Centre for the Development of Industrial Technology (CDTI), with technical and contractual support from the European Space Agency (ESA). HISDESA T together with the Spanish Instituto Nacional de Técnica Aeroespacial (INTA, National Institute for Aerospace Technology) will be responsible for the in-orbit operation and the commercial operation of both satellites, and for the technical management of SEOSAR/Paz on behalf of the MoD. In both cases EADS CASA Espacio (ECE) is the prime contractor leading the industrial consortia. The ground segment development will be assigned to a Spanish consortium. This system is the most important contribution of Spain to the European Programme Global Monitoring for Environment and Security, GMES. This paper presents the Spanish Earth Observation Satellite System focusing on SEOSA T/Ingenio Programme and with special emphasis in the potential contribution to the ESA Third Party Missions Programme and to the Global Monitoring for Environment and Security initiative (GMES) Data Access.

NASA's Earth Observing System Data and Information System - EOSDIS

NASA Technical Reports Server (NTRS)

Ramapriyan, Hampapuram K.

2011-01-01

This slide presentation reviews the work of NASA's Earth Observing System Data and Information System (EOSDIS), a petabyte-scale archive of environmental data that supports global climate change research. The Earth Science Data Systems provide end-to-end capabilities to deliver data and information products to users in support of understanding the Earth system. The presentation contains photographs from space of recent events, (i.e., the effects of the tsunami in Japan, and the wildfires in Australia.) It also includes details of the Data Centers that provide the data to EOSDIS and Science Investigator-led Processing Systems. Information about the Land, Atmosphere Near-real-time Capability for EOS (LANCE) and some of the uses that the system has made possible are reviewed. Also included is information about how to access the data, and evolutionary plans for the future of the system.

A system decomposition approach to the design of functional observers

NASA Astrophysics Data System (ADS)

Fernando, Tyrone; Trinh, Hieu

2014-09-01

This paper reports a system decomposition that allows the construction of a minimum-order functional observer using a state observer design approach. The system decomposition translates the functional observer design problem to that of a state observer for a smaller decomposed subsystem. Functional observability indices are introduced, and a closed-form expression for the minimum order required for a functional observer is derived in terms of those functional observability indices.

The Australian Integrated Marine Observing System

NASA Astrophysics Data System (ADS)

Proctor, R.; Meyers, G.; Roughan, M.; Operators, I.

2008-12-01

The Integrated Marine Observing System (IMOS) is a 92M project established with 50M from the National Collaborative Research Infrastructure Strategy (NCRIS) and co-investments from 10 operators including Universities and government agencies (see below). It is a nationally distributed set of equipment established and maintained at sea, oceanographic data and information services that collectively will contribute to meeting the needs of marine research in both open oceans and over the continental shelf around Australia. In particular, if sustained in the long term, it will permit identification and management of climate change in the marine environment, an area of research that is as yet almost a blank page, studies relevant to conservation of marine biodiversity and research on the role of the oceans in the climate system. While as an NCRIS project IMOS is intended to support research, the data streams are also useful for many societal, environmental and economic applications, such as management of offshore industries, safety at sea, management of marine ecosystems and fisheries and tourism. The infrastructure also contributes to Australia's commitments to international programs of ocean observing and international conventions, such as the 1982 Law of the Sea Convention that established the Australian Exclusive Economic Zone, the United Nations Framework Convention on Climate Change, the Global Ocean Observing System and the intergovernmental coordinating activity Global Earth Observation System of Systems. IMOS is made up of nine national facilities that collect data, using different components of infrastructure and instruments, and two facilities that manage and provide access to data and enhanced data products, one for in situ data and a second for remotely sensed satellite data. The observing facilities include three for the open (bluewater) ocean (Argo Australia, Enhanced Ships of Opportunity and Southern Ocean Time Series), three facilities for coastal

Early outcome for the primary arterial switch operation beyond the age of 3 weeks.

PubMed

Ismail, Sameh R; Kabbani, Mohamed S; Najm, Hani K; Abusuliman, Riyadh M; Elbarbary, Mahmoud

2010-07-01

The arterial switch operation (ASO) for neonates is the standard management for transposition of the great arteries (TGA) with an intact ventricular septum (IVS). Patients presenting for late ASO are at risk due to the possibility of left ventricle (LV) involution. This study aimed to assess the early postoperative course and outcome for children with TGA/IVS and still conditioned LV presenting for late primary ASO. A retrospective study of all TGA/IVS patients who underwent a primary ASO between March 2002 and March 2008 was conducted. The cases were divided into two groups. Group A included all the cases of early ASO repaired before the age of 3 weeks, whereas group B included all the preslected cases of late ASO repaired after the age of 3 weeks. The demographics, intensive care unit (ICU) parameters, complications, and short-term outcomes of the two groups were compared. The study enrolled of 91 patients: 64 patients (70%) in group A and 27 patients (30%) in group B. The mean age was 11 +/- 4 days in group A and 37 +/- 17 days in group B (P < 0.001). The two groups showed no significant statistical differences in ICU parameters, complications, or mortality. For patients with TGA/IVS, ASO still can be tolerated beyond the first month of life in selected cases. Provided the LV still is conditioned, age should not be a limitation for ASO.

Snowmelt and water resources in a changing climate and dustier world

NASA Astrophysics Data System (ADS)

Painter, T. H.

2015-12-01

Snow cover and its melt dominate regional climate and water resources in the world's mountain regions, providing for critical agricultural and sustaining populations in otherwise dry regions. Snowmelt timing and magnitude in mountains tend to be controlled by absorption of solar radiation and snow water equivalent, respectively, and yet both of these are very poorly known even in the best-instrumented mountain regions of the globe. In this talk, we discuss developments in the spaceborne and airborne remote sensing of snow properties, and the assimilation of these products into research water cycle modeling and operational forecasting. Our work with the NWS Colorado Basin River Forecast Center has shown marked improvements in runoff forecasting through inclusion of MODIS and VIIRS fractional snow covered area data. Moreover, the analyses have shown that the CBRFC forecasting errors are strongly sensitive to actual dust radiative forcing in snow with rising limb excursions as large as 40%. With MODIS retrievals of dust radiative forcing, the CBRFC will be implementing modifications to forecasts to reduce those errors to order < 10%. In the last few years, the NASA Airborne Snow Observatory has emerged to provide the first spatially explicit distributions of snow water equivalent and coincident snow albedo products for mountain basins. ASO is an imaging spectrometer and imaging LiDAR system, to quantify snow water equivalent and snow albedo, provide unprecedented knowledge of snow properties, and provide complete, robust inputs to snowmelt runoff models, water management models, and systems of the future. ASO has been flying in the Western US for three snowmelt seasons. In 2015, ASO provided complete basin coverage for the Tuolumne, Merced, Lakes, Rush Creek, and Middle+South Forks of Kings River Basins in the California Sierra Nevada and the Upper Rio Grande, Conejos, and Uncompahgre Basins in the Colorado Rocky Mountains. Analyses show that with ASO data, river

GENERAL EARTHQUAKE-OBSERVATION SYSTEM (GEOS).

USGS Publications Warehouse

Borcherdt, R.D.; Fletcher, Joe B.; Jensen, E.G.; Maxwell, G.L.; VanSchaack, J.R.; Warrick, R.E.; Cranswick, E.; Johnston, M.J.S.; McClearn, R.

1985-01-01

Microprocessor technology has permitted the development of a General Earthquake-Observation System (GEOS) useful for most seismic applications. Central-processing-unit control via robust software of system functions that are isolated on hardware modules permits field adaptability of the system to a wide variety of active and passive seismic experiments and straightforward modification for incorporation of improvements in technology. Various laboratory tests and numerous deployments of a set of the systems in the field have confirmed design goals, including: wide linear dynamic range (16 bit/96 dB); broad bandwidth (36 hr to 600 Hz; greater than 36 hr available); selectable sensor-type (accelerometer, seismometer, dilatometer); selectable channels (1 to 6); selectable record mode (continuous, preset, trigger); large data capacity (1. 4 to 60 Mbytes); selectable time standard (WWVB, master, manual); automatic self-calibration; simple field operation; full capability to adapt system in the field to a wide variety of experiments; low power; portability; and modest costs. System design goals for a microcomputer-controlled system with modular software and hardware components as implemented on the GEOS are presented. The systems have been deployed for 15 experiments, including: studies of near-source strong motion; high-frequency microearthquakes; crustal structure; down-hole wave propagation; teleseismicity; and earth-tidal strains.

A reduced adaptive observer for multivariable systems. [using reduced dynamic ordering

NASA Technical Reports Server (NTRS)

Carroll, R. L.; Lindorff, D. P.

1973-01-01

An adaptive observer for multivariable systems is presented for which the dynamic order of the observer is reduced, subject to mild restrictions. The observer structure depends directly upon the multivariable structure of the system rather than a transformation to a single-output system. The number of adaptive gains is at most the sum of the order of the system and the number of input parameters being adapted. Moreover, for the relatively frequent specific cases for which the number of required adaptive gains is less than the sum of system order and input parameters, the number of these gains is easily determined by inspection of the system structure. This adaptive observer possesses all the properties ascribed to the single-input single-output adpative observer. Like the other adaptive observers some restriction is required of the allowable system command input to guarantee convergence of the adaptive algorithm, but the restriction is more lenient than that required by the full-order multivariable observer. This reduced observer is not restricted to cycle systems.

The wireless networking system of Earthquake precursor mobile field observation

NASA Astrophysics Data System (ADS)

Wang, C.; Teng, Y.; Wang, X.; Fan, X.; Wang, X.

2012-12-01

The mobile field observation network could be real-time, reliably record and transmit large amounts of data, strengthen the physical signal observations in specific regions and specific period, it can improve the monitoring capacity and abnormal tracking capability. According to the features of scatter everywhere, a large number of current earthquake precursor observation measuring points, networking technology is based on wireless broadband accessing McWILL system, the communication system of earthquake precursor mobile field observation would real-time, reliably transmit large amounts of data to the monitoring center from measuring points through the connection about equipment and wireless accessing system, broadband wireless access system and precursor mobile observation management center system, thereby implementing remote instrument monitoring and data transmition. At present, the earthquake precursor field mobile observation network technology has been applied to fluxgate magnetometer array geomagnetic observations of Tianzhu, Xichang,and Xinjiang, it can be real-time monitoring the working status of the observational instruments of large area laid after the last two or three years, large scale field operation. Therefore, it can get geomagnetic field data of the local refinement regions and provide high-quality observational data for impending earthquake tracking forecast. Although, wireless networking technology is very suitable for mobile field observation with the features of simple, flexible networking etc, it also has the phenomenon of packet loss etc when transmitting a large number of observational data due to the wireless relatively weak signal and narrow bandwidth. In view of high sampling rate instruments, this project uses data compression and effectively solves the problem of data transmission packet loss; Control commands, status data and observational data transmission use different priorities and means, which control the packet loss rate within

Merging Radar Quantitative Precipitation Estimates (QPEs) from the High-resolution NEXRAD Reanalysis over CONUS with Rain-gauge Observations

NASA Astrophysics Data System (ADS)

Prat, O. P.; Nelson, B. R.; Stevens, S. E.; Nickl, E.; Seo, D. J.; Kim, B.; Zhang, J.; Qi, Y.

2015-12-01

The processing of radar-only precipitation via the reanalysis from the National Mosaic and Multi-Sensor Quantitative (NMQ/Q2) based on the WSR-88D Next-generation Radar (Nexrad) network over the Continental United States (CONUS) is completed for the period covering from 2002 to 2011. While this constitutes a unique opportunity to study precipitation processes at higher resolution than conventionally possible (1-km, 5-min), the long-term radar-only product needs to be merged with in-situ information in order to be suitable for hydrological, meteorological and climatological applications. The radar-gauge merging is performed by using rain gauge information at daily (Global Historical Climatology Network-Daily: GHCN-D), hourly (Hydrometeorological Automated Data System: HADS), and 5-min (Automated Surface Observing Systems: ASOS; Climate Reference Network: CRN) resolution. The challenges related to incorporating differing resolution and quality networks to generate long-term large-scale gridded estimates of precipitation are enormous. In that perspective, we are implementing techniques for merging the rain gauge datasets and the radar-only estimates such as Inverse Distance Weighting (IDW), Simple Kriging (SK), Ordinary Kriging (OK), and Conditional Bias-Penalized Kriging (CBPK). An evaluation of the different radar-gauge merging techniques is presented and we provide an estimate of uncertainty for the gridded estimates. In addition, comparisons with a suite of lower resolution QPEs derived from ground based radar measurements (Stage IV) are provided in order to give a detailed picture of the improvements and remaining challenges.

Observing System Forecast Experiments at the DAO

NASA Technical Reports Server (NTRS)

Atlas, Robert

2001-01-01

Since the advent of meteorological satellites in the 1960's, numerous experiments have been conducted in order to evaluate the impact of these and other data on atmospheric analysis and prediction. Such studies have included both OSE'S and OSSE's. The OSE's were conducted to evaluate the impact of specific observations or classes of observations on analyses and forecasts. Such experiments have been performed for selected types of conventional data and for various satellite data sets as they became available. (See for example the 1989 ECMWF/EUMETSAT workshop proceedings on "The use of satellite data in operational numerical weather prediction" and the references contained therein.) The ODYSSEY were conducted to evaluate the potential for future observing systems to improve Numerical Weather Prediction NWP and to plan for the Global Weather Experiment and more recently for EVANS (Atlas et al., 1985a; Arnold and Day, 1986; Hoffman et al., 1990). In addition, OSSE's have been run to evaluate trade-offs in the design of observing systems and observing networks (Atlas and Emmitt, 1991; Rohaly and Krishnamurti, 1993), and to test new methodology for data assimilation (Atlas and Bloom, 1989).

NASA's Earth Observing Data and Information System

NASA Technical Reports Server (NTRS)

Mitchell, Andrew E.; Behnke, Jeanne; Lowe, Dawn; Ramapriyan, H. K.

2009-01-01

NASA's Earth Observing System Data and Information System (EOSDIS) has been a central component of NASA Earth observation program for over 10 years. It is one of the largest civilian science information system in the US, performing ingest, archive and distribution of over 3 terabytes of data per day much of which is from NASA s flagship missions Terra, Aqua and Aura. The system supports a variety of science disciplines including polar processes, land cover change, radiation budget, and most especially global climate change. The EOSDIS data centers, collocated with centers of science discipline expertise, archive and distribute standard data products produced by science investigator-led processing systems. Key to the success of EOSDIS is the concept of core versus community requirements. EOSDIS supports a core set of services to meet specific NASA needs and relies on community-developed services to meet specific user needs. EOSDIS offers a metadata registry, ECHO (Earth Observing System Clearinghouse), through which the scientific community can easily discover and exchange NASA s Earth science data and services. Users can search, manage, and access the contents of ECHO s registries (data and services) through user-developed and community-tailored interfaces or clients. The ECHO framework has become the primary access point for cross-Data Center search-and-order of EOSDIS and other Earth Science data holdings archived at the EOSDIS data centers. ECHO s Warehouse Inventory Search Tool (WIST) is the primary web-based client for discovering and ordering cross-discipline data from the EOSDIS data centers. The architecture of the EOSDIS provides a platform for the publication, discovery, understanding and access to NASA s Earth Observation resources and allows for easy integration of new datasets. The EOSDIS also has developed several methods for incorporating socioeconomic data into its data collection. Over the years, we have developed several methods for determining

NASDA's earth observation satellite data archive policy for the earth observation data and information system (EOIS)

NASA Technical Reports Server (NTRS)

Sobue, Shin-ichi; Yoshida, Fumiyoshi; Ochiai, Osamu

1996-01-01

NASDA's new Advanced Earth Observing Satellite (ADEOS) is scheduled for launch in August, 1996. ADEOS carries 8 sensors to observe earth environmental phenomena and sends their data to NASDA, NASA, and other foreign ground stations around the world. The downlink data bit rate for ADEOS is 126 MB/s and the total volume of data is about 100 GB per day. To archive and manage such a large quantity of data with high reliability and easy accessibility it was necessary to develop a new mass storage system with a catalogue information database using advanced database management technology. The data will be archived and maintained in the Master Data Storage Subsystem (MDSS) which is one subsystem in NASDA's new Earth Observation data and Information System (EOIS). The MDSS is based on a SONY ID1 digital tape robotics system. This paper provides an overview of the EOIS system, with a focus on the Master Data Storage Subsystem and the NASDA Earth Observation Center (EOC) archive policy for earth observation satellite data.

Strategies for In Vivo Screening and Mitigation of Hepatotoxicity Associated with Antisense Drugs.

PubMed

Kamola, Piotr J; Maratou, Klio; Wilson, Paul A; Rush, Kay; Mullaney, Tanya; McKevitt, Tom; Evans, Paula; Ridings, Jim; Chowdhury, Probash; Roulois, Aude; Fairchild, Ann; McCawley, Sean; Cartwright, Karen; Gooderham, Nigel J; Gant, Timothy W; Moores, Kitty; Hughes, Stephen A; Edbrooke, Mark R; Clark, Kenneth; Parry, Joel D

2017-09-15

Antisense oligonucleotide (ASO) gapmers downregulate gene expression by inducing enzyme-dependent degradation of targeted RNA and represent a promising therapeutic platform for addressing previously undruggable genes. Unfortunately, their therapeutic application, particularly that of the more potent chemistries (e.g., locked-nucleic-acid-containing gapmers), has been hampered by their frequent hepatoxicity, which could be driven by hybridization-mediated interactions. An early de-risking of this liability is a crucial component of developing safe, ASO-based drugs. To rank ASOs based on their effect on the liver, we have developed an acute screen in the mouse that can be applied early in the drug development cycle. A single-dose (3-day) screen with streamlined endpoints (i.e., plasma transaminase levels and liver weights) was observed to be predictive of ASO hepatotoxicity ranking established based on a repeat-dose (15 day) study. Furthermore, to study the underlying mechanisms of liver toxicity, we applied transcriptome profiling and pathway analyses and show that adverse in vivo liver phenotypes correlate with the number of potent, hybridization-mediated off-target effects (OTEs). We propose that a combination of in silico OTE predictions, streamlined in vivo hepatotoxicity screening, and a transcriptome-wide selectivity screen is a valid approach to identifying and progressing safer compounds. Copyright © 2017 GSK R&D. Published by Elsevier Inc. All rights reserved.

HARMFUL ALGAL BLOOMS OBSERVING SYSTEM PILOT PROJECT

EPA Science Inventory

The HABSOS Pilot Project is being developed through a partnership of federal, state and academic organizations as proof-of-concept for a coastal observing system in the Gulf of Mexico. The goal is to design a HAB data management system and develop the regional communication infra...

Earth Observation System Flight Dynamics System Covariance Realism

NASA Technical Reports Server (NTRS)

Zaidi, Waqar H.; Tracewell, David

2016-01-01

This presentation applies a covariance realism technique to the National Aeronautics and Space Administration (NASA) Earth Observation System (EOS) Aqua and Aura spacecraft based on inferential statistics. The technique consists of three parts: collection calculation of definitive state estimates through orbit determination, calculation of covariance realism test statistics at each covariance propagation point, and proper assessment of those test statistics.

Weather Observation Systems and Efficiency of Fighting Forest Fires

NASA Astrophysics Data System (ADS)

Khabarov, N.; Moltchanova, E.; Obersteiner, M.

2007-12-01

Weather observation is an essential component of modern forest fire management systems. Satellite and in-situ based weather observation systems might help to reduce forest loss, human casualties and destruction of economic capital. In this paper, we develop and apply a methodology to assess the benefits of various weather observation systems on reductions of burned area due to early fire detection. In particular, we consider a model where the air patrolling schedule is determined by a fire hazard index. The index is computed from gridded daily weather data for the area covering parts Spain and Portugal. We conduct a number of simulation experiments. First, the resolution of the original data set is artificially reduced. The reduction of the total forest burned area associated with air patrolling based on a finer weather grid indicates the benefit of using higher spatially resolved weather observations. Second, we consider a stochastic model to simulate forest fires and explore the sensitivity of the model with respect to the quality of input data. The analysis of combination of satellite and ground monitoring reveals potential cost saving due to a "system of systems effect" and substantial reduction in burned area. Finally, we estimate the marginal improvement schedule for loss of life and economic capital as a function of the improved fire observing system.

Rescue of peripheral vestibular function in Usher syndrome mice using a splice-switching antisense oligonucleotide.

PubMed

Vijayakumar, Sarath; Depreux, Frederic F; Jodelka, Francine M; Lentz, Jennifer J; Rigo, Frank; Jones, Timothy A; Hastings, Michelle L

2017-09-15

Usher syndrome type 1C (USH1C/harmonin) is associated with profound retinal, auditory and vestibular dysfunction. We have previously reported on an antisense oligonucleotide (ASO-29) that dramatically improves auditory function and balance behavior in mice homozygous for the harmonin mutation Ush1c c.216G > A following a single systemic administration. The findings were suggestive of improved vestibular function; however, no direct vestibular assessment was made. Here, we measured vestibular sensory evoked potentials (VsEPs) to directly assess vestibular function in Usher mice. We report that VsEPs are absent or abnormal in Usher mice, indicating profound loss of vestibular function. Strikingly, Usher mice receiving ASO-29 treatment have normal or elevated vestibular response thresholds when treated during a critical period between postnatal day 1 and 5, respectively. In contrast, treatment of mice with ASO-29 treatment at P15 was minimally effective at rescuing vestibular function. Interestingly, ASO-29 treatment at P1, P5 or P15 resulted in sufficient vestibular recovery to support normal balance behaviors, suggesting a therapeutic benefit to balance with ASO-29 treatment at P15 despite the profound vestibular functional deficits that persist with treatment at this later time. These findings provide the first direct evidence of an effective treatment of peripheral vestibular function in a mouse model of USH1C and reveal the potential for using antisense technology to treat vestibular dysfunction. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Resource utilization after introduction of a standardized clinical assessment and management plan.

PubMed

Friedman, Kevin G; Rathod, Rahul H; Farias, Michael; Graham, Dionne; Powell, Andrew J; Fulton, David R; Newburger, Jane W; Colan, Steven D; Jenkins, Kathy J; Lock, James E

2010-01-01

A Standardized Clinical Assessment and Management Plan (SCAMP) is a novel quality improvement initiative that standardizes the assessment and management of all patients who carry a predefined diagnosis. Based on periodic review of systemically collected data the SCAMP is designed to be modified to improve its own algorithm. One of the objectives of a SCAMP is to identify and reduce resource utilization and patient care costs. We retrospectively reviewed resource utilization in the first 93 arterial switch operation (ASO) SCAMP patients and 186 age-matched control ASO patients. We compared diagnostic and laboratory testing obtained at the initial SCAMP clinic visit and control patient visits. To evaluate the effect of the SCAMP over time, the number of clinic visits per patient year and echocardiograms per patient year in historical control ASO patients were compared to the projected rates for ASO SCAMP participants. Cardiac magnetic resonance imaging (MRI), stress echocardiogram, and lipid profile utilization were higher in the initial SCAMP clinic visit group than in age-matched control patients. Total echocardiogram and lung scan usage were similar. Chest X-ray and exercise stress testing were obtained less in SCAMP patients. ASO SCAMP patients are projected to have 0.5 clinic visits and 0.5 echocardiograms per year. Historical control patients had more clinic visits (1.2 vs. 0.5 visits/patient year, P<.01) and a higher echocardiogram rate (0.92 vs. 0.5 echocardiograms/patient year, P<.01) Implementation of a SCAMP may initially lead to increased resource utilization, but over time resource utilization is projected to decrease.

RNA splicing process analysis for identifying antisense oligonucleotide inhibitors with padlock probe-based isothermal amplification.

PubMed

Ren, Xiaojun; Deng, Ruijie; Wang, Lida; Zhang, Kaixiang; Li, Jinghong

2017-08-01

RNA splicing, which mainly involves two transesterification steps, is a fundamental process of gene expression and its abnormal regulation contributes to serious genetic diseases. Antisense oligonucleotides (ASOs) are genetic control tools that can be used to specifically control genes through alteration of the RNA splicing pathway. Despite intensive research, how ASOs or various other factors influence the multiple processes of RNA splicing still remains obscure. This is largely due to an inability to analyze the splicing efficiency of each step in the RNA splicing process with high sensitivity. We addressed this limitation by introducing a padlock probe-based isothermal amplification assay to achieve quantification of the specific products in different splicing steps. With this amplified assay, the roles that ASOs play in RNA splicing inhibition in the first and second steps could be distinguished. We identified that 5'-ASO could block RNA splicing by inhibiting the first step, while 3'-ASO could block RNA splicing by inhibiting the second step. This method provides a versatile tool for assisting efficient ASO design and discovering new splicing modulators and therapeutic drugs.

RNA splicing process analysis for identifying antisense oligonucleotide inhibitors with padlock probe-based isothermal amplification† †Electronic supplementary information (ESI) available: Additional experimental materials, methods, DNA sequences and supplementary figures and tables. See DOI: 10.1039/c7sc01336a Click here for additional data file.

PubMed Central

Ren, Xiaojun; Deng, Ruijie; Wang, Lida; Zhang, Kaixiang

2017-01-01

RNA splicing, which mainly involves two transesterification steps, is a fundamental process of gene expression and its abnormal regulation contributes to serious genetic diseases. Antisense oligonucleotides (ASOs) are genetic control tools that can be used to specifically control genes through alteration of the RNA splicing pathway. Despite intensive research, how ASOs or various other factors influence the multiple processes of RNA splicing still remains obscure. This is largely due to an inability to analyze the splicing efficiency of each step in the RNA splicing process with high sensitivity. We addressed this limitation by introducing a padlock probe-based isothermal amplification assay to achieve quantification of the specific products in different splicing steps. With this amplified assay, the roles that ASOs play in RNA splicing inhibition in the first and second steps could be distinguished. We identified that 5′-ASO could block RNA splicing by inhibiting the first step, while 3′-ASO could block RNA splicing by inhibiting the second step. This method provides a versatile tool for assisting efficient ASO design and discovering new splicing modulators and therapeutic drugs. PMID:28989608

Compatibility of ASO3-adjuvanted H1N1pdm09 and seasonal trivalent influenza vaccines in adults: results of a randomized, controlled trial.

PubMed

Scheifele, David W; Ward, Brian J; Dionne, Marc; Vanderkooi, Otto G; Loeb, Mark; Coleman, Brenda L; Li, Yan

2012-07-06

When Canada chose a novel adjuvanted vaccine to combat the 2009 influenza pandemic, seasonal trivalent inactivated vaccine (TIV) was also available but compatibility of the two had not been assessed. To compare responses after concurrent or sequential administration of these vaccines, adults 20-59 years old were randomly assigned (1:1) to receive ASO3-adjuvanted H1N1pdm09 vaccine (Arepanrix, GSK, Quebec City, Quebec), with TIV (Vaxigrip, Sanofi Pasteur, Toronto) given concurrently or 21 days later. Blood was obtained at baseline and 21 days after each vaccination to measure hemagglutination inhibition (HAI) titers. Adverse effects were assessed using symptom diaries and personal interviews. 282 participants completed the study (concurrent vaccines 145, sequential vaccines 137). HAI titers to H1N1pdm09 were ≥ 40 at baseline in 15-18% of participants and following vaccination in 91-92%. Initially seropositive subjects (titer ≥ 10) had lower H1N1pdm09 geometric mean HAI titers (GMT) after concurrent than separate vaccinations (320.0 vs 476.5, p=0.039) but both exceeded GM responses of initially naïve participants, which were unaffected by concurrent TIV. Responses to TIV were not lower after concurrent than separate vaccination. Adverse event rates were not increased by concurrent vaccinations above those with H1N1pdm09 vaccine alone. This adjuvanted H1N1pdm09 vaccine was immunogenic and compatible with concurrently administered TIV. Copyright © 2012 Elsevier Ltd. All rights reserved.

Current systematic carbon-cycle observations and the need for implementing a policy-relevant carbon observing system

NASA Astrophysics Data System (ADS)

Ciais, P.; Dolman, A. J.; Bombelli, A.; Duren, R.; Peregon, A.; Rayner, P. J.; Miller, C.; Gobron, N.; Kinderman, G.; Marland, G.; Gruber, N.; Chevallier, F.; Andres, R. J.; Balsamo, G.; Bopp, L.; Bréon, F.-M.; Broquet, G.; Dargaville, R.; Battin, T. J.; Borges, A.; Bovensmann, H.; Buchwitz, M.; Butler, J.; Canadell, J. G.; Cook, R. B.; DeFries, R.; Engelen, R.; Gurney, K. R.; Heinze, C.; Heimann, M.; Held, A.; Henry, M.; Law, B.; Luyssaert, S.; Miller, J.; Moriyama, T.; Moulin, C.; Myneni, R. B.; Nussli, C.; Obersteiner, M.; Ojima, D.; Pan, Y.; Paris, J.-D.; Piao, S. L.; Poulter, B.; Plummer, S.; Quegan, S.; Raymond, P.; Reichstein, M.; Rivier, L.; Sabine, C.; Schimel, D.; Tarasova, O.; Valentini, R.; Wang, R.; van der Werf, G.; Wickland, D.; Williams, M.; Zehner, C.

2014-07-01

A globally integrated carbon observation and analysis system is needed to improve the fundamental understanding of the global carbon cycle, to improve our ability to project future changes, and to verify the effectiveness of policies aiming to reduce greenhouse gas emissions and increase carbon sequestration. Building an integrated carbon observation system requires transformational advances from the existing sparse, exploratory framework towards a dense, robust, and sustained system in all components: anthropogenic emissions, the atmosphere, the ocean, and the terrestrial biosphere. The paper is addressed to scientists, policymakers, and funding agencies who need to have a global picture of the current state of the (diverse) carbon observations. We identify the current state of carbon observations, and the needs and notional requirements for a global integrated carbon observation system that can be built in the next decade. A key conclusion is the substantial expansion of the ground-based observation networks required to reach the high spatial resolution for CO2 and CH4 fluxes, and for carbon stocks for addressing policy-relevant objectives, and attributing flux changes to underlying processes in each region. In order to establish flux and stock diagnostics over areas such as the southern oceans, tropical forests, and the Arctic, in situ observations will have to be complemented with remote-sensing measurements. Remote sensing offers the advantage of dense spatial coverage and frequent revisit. A key challenge is to bring remote-sensing measurements to a level of long-term consistency and accuracy so that they can be efficiently combined in models to reduce uncertainties, in synergy with ground-based data. Bringing tight observational constraints on fossil fuel and land use change emissions will be the biggest challenge for deployment of a policy-relevant integrated carbon observation system. This will require in situ and remotely sensed data at much higher

Therapeutic silencing of FSP27 reduces the progression of atherosclerosis in Ldlr-/- mice.

PubMed

Rajamoorthi, Ananthi; Lee, Richard G; Baldán, Ángel

2018-05-24

Obesity, hepatosteatosis, and hypertriglyceridemia are components of the metabolic syndrome and independent risk factors for cardiovascular disease. The lipid droplet-associated protein CIDEC (cell death-inducing DFFA-like effector C), known in mice as FSP27 (fat-specific protein 27), plays a key role in maintaining triacylglyceride (TAG) homeostasis in adipose tissue and liver, and controls circulating TAG levels in mice. Importantly, mutations and SNPs in CIDEC are associated with dyslipidemia and altered metabolic function in humans. Here we tested whether systemic silencing of Fsp27 using antisense oligonucleotides (ASOs) was atheroprotective in LDL receptor knock-out (Ldlr -/- ) mice. Atheroprone Ldlr -/- mice were fed a high-fat, high-cholesterol diet for 12 weeks while simultaneously dosed with saline, ASO-ctrl, or ASO-Fsp27. Data show that, compared to control treatments, silencing Fsp27 significantly reduced body weight gain and visceral adiposity, prevented diet-induced hypertriglyceridemia, and reduced atherosclerotic lesion size both in en face aortas and in the aortic root. Our findings suggest that therapeutic silencing of Fsp27 with ASOs may be beneficial in the prevention and management of atherogenic disease in patients with metabolic syndrome. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Incorporating Parallel Computing into the Goddard Earth Observing System Data Assimilation System (GEOS DAS)

NASA Technical Reports Server (NTRS)

Larson, Jay W.

1998-01-01

Atmospheric data assimilation is a method of combining actual observations with model forecasts to produce a more accurate description of the earth system than the observations or forecast alone can provide. The output of data assimilation, sometimes called the analysis, are regular, gridded datasets of observed and unobserved variables. Analysis plays a key role in numerical weather prediction and is becoming increasingly important for climate research. These applications, and the need for timely validation of scientific enhancements to the data assimilation system pose computational demands that are best met by distributed parallel software. The mission of the NASA Data Assimilation Office (DAO) is to provide datasets for climate research and to support NASA satellite and aircraft missions. The system used to create these datasets is the Goddard Earth Observing System Data Assimilation System (GEOS DAS). The core components of the the GEOS DAS are: the GEOS General Circulation Model (GCM), the Physical-space Statistical Analysis System (PSAS), the Observer, the on-line Quality Control (QC) system, the Coupler (which feeds analysis increments back to the GCM), and an I/O package for processing the large amounts of data the system produces (which will be described in another presentation in this session). The discussion will center on the following issues: the computational complexity for the whole GEOS DAS, assessment of the performance of the individual elements of GEOS DAS, and parallelization strategy for some of the components of the system.

Virtual Observation System for Earth System Model: An Application to ACME Land Model Simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Dali; Yuan, Fengming; Hernandez, Benjamin

Investigating and evaluating physical-chemical-biological processes within an Earth system model (EMS) can be very challenging due to the complexity of both model design and software implementation. A virtual observation system (VOS) is presented to enable interactive observation of these processes during system simulation. Based on advance computing technologies, such as compiler-based software analysis, automatic code instrumentation, and high-performance data transport, the VOS provides run-time observation capability, in-situ data analytics for Earth system model simulation, model behavior adjustment opportunities through simulation steering. A VOS for a terrestrial land model simulation within the Accelerated Climate Modeling for Energy model is also presentedmore » to demonstrate the implementation details and system innovations.« less

Virtual Observation System for Earth System Model: An Application to ACME Land Model Simulations

DOE PAGES

Wang, Dali; Yuan, Fengming; Hernandez, Benjamin; ...

2017-01-01

Investigating and evaluating physical-chemical-biological processes within an Earth system model (EMS) can be very challenging due to the complexity of both model design and software implementation. A virtual observation system (VOS) is presented to enable interactive observation of these processes during system simulation. Based on advance computing technologies, such as compiler-based software analysis, automatic code instrumentation, and high-performance data transport, the VOS provides run-time observation capability, in-situ data analytics for Earth system model simulation, model behavior adjustment opportunities through simulation steering. A VOS for a terrestrial land model simulation within the Accelerated Climate Modeling for Energy model is also presentedmore » to demonstrate the implementation details and system innovations.« less

Observation and integrated Earth-system science: A roadmap for 2016-2025

NASA Astrophysics Data System (ADS)

Simmons, Adrian; Fellous, Jean-Louis; Ramaswamy, Venkatachalam; Trenberth, Kevin; Asrar, Ghassem; Balmaseda, Magdalena; Burrows, John P.; Ciais, Philippe; Drinkwater, Mark; Friedlingstein, Pierre; Gobron, Nadine; Guilyardi, Eric; Halpern, David; Heimann, Martin; Johannessen, Johnny; Levelt, Pieternel F.; Lopez-Baeza, Ernesto; Penner, Joyce; Scholes, Robert; Shepherd, Ted

2016-05-01

This report is the response to a request by the Committee on Space Research of the International Council for Science to prepare a roadmap on observation and integrated Earth-system science for the coming ten years. Its focus is on the combined use of observations and modelling to address the functioning, predictability and projected evolution of interacting components of the Earth system on timescales out to a century or so. It discusses how observations support integrated Earth-system science and its applications, and identifies planned enhancements to the contributing observing systems and other requirements for observations and their processing. All types of observation are considered, but emphasis is placed on those made from space. The origins and development of the integrated view of the Earth system are outlined, noting the interactions between the main components that lead to requirements for integrated science and modelling, and for the observations that guide and support them. What constitutes an Earth-system model is discussed. Summaries are given of key cycles within the Earth system. The nature of Earth observation and the arrangements for international coordination essential for effective operation of global observing systems are introduced. Instances are given of present types of observation, what is already on the roadmap for 2016-2025 and some of the issues to be faced. Observations that are organised on a systematic basis and observations that are made for process understanding and model development, or other research or demonstration purposes, are covered. Specific accounts are given for many of the variables of the Earth system. The current status and prospects for Earth-system modelling are summarized. The evolution towards applying Earth-system models for environmental monitoring and prediction as well as for climate simulation and projection is outlined. General aspects of the improvement of models, whether through refining the

Theoretical studies of arsenite adsorption and its oxidation mechanism on a perfect TiO 2 anatase (1 0 1) surface

NASA Astrophysics Data System (ADS)

Wei, Zhigang; Zhang, Shaowen; Pan, Zhanchang; Liu, Yue

2011-11-01

There are many areas in the world where the ground water has been contaminated by arsenic. TiO2 is one of the most promising materials that can remove arsenic from groundwater supplies by the adsorption-based processes. The TiO2 surface is capable of photo-catalytic oxidation (PCO) changing the arsenite [As(III)] to arsenate [As(V)] which is more easily absorbed by the surface, increasing the efficiency of the process. In this paper, a density functional theory calculation has been performed to investigate the adsorption of As(III) on a perfect TiO2 anatase (1 0 1) surface. All the As(III) solution species such as H3AsO3, H2AsO3-, HAsO32- and AsO33- are put onto the surface with many different possible attitudes to obtain the adsorption energy. Based on the adsorption energy and the concentration of H3AsO3, H2AsO3-, HAsO32- and AsO33- in an aqueous solution, the bidentate binuclear (BB) adsorption configurations of H2AsO3- on the surface are more favorable at low As(III) concentrations, whereas BB form and monodentate mononuclear (MM) form may coexist at higher concentrations. By calculating H2AsO3- co-adsorption with water and oxygen, we can confirm the deep acceptor character of an adsorbed O2 molecule which implies that surface superoxide (or hydroperoxyl radical) plays an important role during the PCO process of As(III) on TiO2 surface.

Do the filarial lymphoedema patients''3 months recall' on acute dermato-lymphangio-adenitis (ADLA) correlate with streptococcal serology?

PubMed

De Britto, L J; Das, L K

2012-08-01

The aim of this study was to know the correlation of patients' 3 months recall on acute dermato-lymphangio-adenitis (ADLA) with anti-streptolysin O (ASO) serology and its application as a tool to know the burden of ADLA in the community. Fifty-nine lymphoedema (LE) patients and 27 age matched controls were clinically assessed for LE and the occurrence of ADLA during the previous 3 months was obtained by recall. After obtaining the informed consent, 2 mL of venous blood sample was collected and ASO was quantified in Olympus AU400 auto-analyzer. When the results were computed as two groups, controls and LE patients with no reported ADLA and LE patients with reported ADLA (by 3 months recall), the ASO positivity and ASO titre was significantly higher in the later group (P < 0.05). When the results were computed as three groups, controls with no reported ADLA, LE patients with no reported ADLA and LE patients with reported ADLA, the ASO titre was significantly higher in LE patients reported ADLA (P < 0.05). As ASO was measured in post-infection phase, we relied on the ASO titre for making conclusion. Patients' 3 months recall on ADLA correlates with the ASO titre and therefore, it could be considered as a tool to measure the burden of ADLA in the community. Multicentre community-based studies are needed to ascertain the findings. © 2011 The Authors. Journal of the European Academy of Dermatology and Venereology © 2011 European Academy of Dermatology and Venereology.

Motives for meaningful involvement in rural AIDS service organizations

PubMed Central

Paterson, Barbara L.; Ross, Steven; Gaudet, Ted

2013-01-01

The research described herein was a three-year exploratory descriptive study to examine how meaningful involvement (MIPA) is conceptualized and experienced in rural regions of the Maritime provinces of Canada. The focus of this paper is one aspect of the research; i.e., what motivates the clients of AIDS Service Organizations (ASOs) in rural Canada to become meaningfully involved? We interviewed 34 people who were past or current clients of ASOs in Maritime Canada and who self-reported as engaging in at-risk behaviors for HIV or living with HIV. The interviews explored participants' perspectives about their motives for becoming meaningfully involved in an ASO. Three themes regarding motives for MIPA were revealed: (1) meeting personal needs; (2) making a difference to others; and (3) recognizing a fit between their skills, goals, needs and the opportunities and experiences within the ASO and with other ASO clients. Participants generally cited more than one motive. This research study contributed to the field of knowledge about the motives for MIPA in which it reveals (1) that MIPA was conceptualized by the rural ASO clients as whatever participation provided them personal meaning (i.e., by fulfilling a personal need, by making a difference, and by recognizing a fit) and (2) the important role that ASO staff and volunteers have in fostering and sustaining MIPA. The study also highlighted a trajectory of involvements that support the need for ASOs to entertain a wide range of roles that are assumed as MIPA. PMID:24111835

Nonlinear observer designs for fuel cell power systems

NASA Astrophysics Data System (ADS)

Gorgun, Haluk

A fuel cell is an electrochemical device that combines hydrogen and oxygen, with the aid of electro-catalysts, to produce electricity. A fuel cell consists of a negatively charged anode, a positively charged cathode and an electrolyte, which transports protons or ions. A low temperature fuel cell has an electrical potential of about 0.7 Volt when generating a current density of 300--500 mA/cm2. Practical fuel cell power systems will require a combination of several cells in series (a stack) to satisfy the voltage requirements of specific applications. Fuel cells are suitable for a potentially wide variety of applications, from stationary power generation in the range of hundreds of megawatts to portable electronics in the range of a couple of watts. Efficient operation of a fuel cell system requires advanced feedback control designs. Reliable measurements from the system are necessary to implement such designs. However, most of the commercially available sensors do not operate properly in the reformate and humidified gas streams in fuel cell systems. Sensors working varying degrees of success are too big and costly, and sensors that are potentially low cost are not reliable or do not have the required life time [28]. Observer designs would eliminate sensor needs for measurements, and make feedback control implementable. Since the fuel cell system dynamics are highly nonlinear, observer design is not an easy task. In this study we aim to develop nonlinear observer design methods applicable to fuel cell systems. In part I of the thesis we design an observer to estimate the hydrogen partial pressure in the anode channel. We treat inlet partial pressure as an unknown slowly varying parameter and develop an adaptive observer that employs a nonlinear voltage injection term. However in this design Fuel Processing System (FPS) dynamics are not modelled, and their effect on the anode dynamics are treated as plant uncertainty. In part II of the thesis we study the FPS

Carbon Dioxide Observational Platform System (CO-OPS), feasibility study

NASA Technical Reports Server (NTRS)

Bouquet, D. L.; Hall, D. W.; Mcelveen, R. P.

1987-01-01

The Carbon Dioxide Observational Platform System (CO-OPS) is a near-space, geostationary, multi-user, unmanned microwave powered monitoring platform system. This systems engineering feasibility study addressed identified existing requirements such as: carbon dioxide observational data requirements, communications requirements, and eye-in-the-sky requirements of other groups like the Defense Department, the Forestry Service, and the Coast Guard. In addition, potential applications in: earth system science, space system sciences, and test and verification (satellite sensors and data management techniques) were considered. The eleven month effort is summarized. Past work and methods of gathering the required observational data were assessed and rough-order-of magnitude cost estimates have shown the CO-OPS system to be most cost effective (less than $30 million within a 10 year lifetime). It was also concluded that there are no technical, schedule, or obstacles that would prevent achieving the objectives of the total 5-year CO-OPS program.

JWST Planetary Observations Within the Solar System

NASA Technical Reports Server (NTRS)

Lunine, Jonathan; Hammel, Heidi; Schaller, Emily; Sonneborn, George; Orton, Glenn; Rieke, George; Rieke, Marcia

2010-01-01

JWST provides capabilities unmatched by other telescopic facilities in the near to mid infrared part of the electromagnetic spectrum. Its combination of broad wavelength range, high sensitivity and near diffraction-limited imaging around two microns wavelength make it a high value facility for a variety of Solar System targets. Beyond Neptune, a class of cold, large bodies that include Pluto, Triton and Eris exhibits surface deposits of nitrogen, methane, and other molecules that are poorly observed from the ground, but for which JWST might provide spectral mapping at high sensitivity and spatial resolution difficult to match with the current generation of ground-based observatories. The observatory will also provide unique sensitivity in a variety of near and mid infrared windows for observing relatively deep into the atmospheres of Uranus and Neptune, searching there for minor species. It will examine the Jovian aurora in a wavelength regime where the background atmosphere is dark. Special provision of a subarray observing strategy may allow observation of Jupiter and Saturn over a larger wavelength range despite their large surface brightnesses, allowing for detailed observation of transient phenomena including large scale storms and impact-generation disturbances. JWST's observations of Saturn's moon Titan will overlap with and go beyond the 2017 end-of-mission for Cassini, providing an important extension to the time-series of meteorological studies for much of northern hemisphere summer. It will overlap with a number of other planetary missions to targets for which JWST can make unique types of observations. JWST provides a platform for linking solar system and extrasolar planet studies through its unique observational capabilities in both arenas.

Computerized Systems for Collecting Real-Time Observational Data.

ERIC Educational Resources Information Center

Kahng, SungWoo; Iwata, Brian

1998-01-01

A survey of 15 developers of computerized real-time observation systems found many systems have incorporated laptop or handheld computers as well as bar-code scanners. Most systems used IBM-compatible software, and ranged from free to complete systems costing more than $1,500. Data analysis programs were included with most programs. (Author/CR)

A net-centric system of services model for the Integrated Earth Observation System (IEOS) and the Integrated Ocean Observing System (IOOS)

NASA Astrophysics Data System (ADS)

Ardanuy, Philip; Bensman, Ed; Bergen, Bill; Chen, Bob; Griffith, Frank; Sutton, Cary; Hood, Carroll; Ritchie, Adrian; Tarro, Andre

2006-08-01

This paper considers an evolved technique for significantly enhanced enterprise-level data processing, reprocessing, archival, dissemination, and utilization. There is today a robust working paradigm established with the Advanced Weather Interactive Processing System (AWIPS)-NOAA/NWS's information integration and fusion capability. This process model extends vertically, and seamlessly, from environmental sensing through the direct delivery of societal benefit. NWS, via AWIPS, is the primary source of weather forecast and warning information in the nation. AWIPS is the tested and proven "the nerve center of operations" at all 122 NWS Weather Forecast Offices (WFOs) and 13 River Forecast Centers (RFCs). However, additional line organizations whose role in satisfying NOAA's five mission goals (ecosystems, climate, weather & water, commerce & transportation, and mission support) in multiple program areas might be facilitated through utilization of AWIPS-like functionalities, including the National Marine Fisheries Service (NMFS); National Environmental Satellite, Data, and Information Service (NESDIS); Office of Oceanic & Atmospheric Research (OAR); and the National Ocean Service (NOS). In addition to NOAA's mission goals, there are nine diverse, recommended, and important societal benefit areas in the US Integrated Earth Observation System (IEOS). This paper shows how the satisfaction of this suite of goals and benefit areas can be optimized by leveraging several key ingredients: (1) the evolution of AWIPS towards a net-centric system of services concept of operations; (2) infusion of technologies and concepts from pathfinder systems; (3) the development of new observing systems targeted at deliberate, and not just serendipitous, societal benefit; and (4) the diverse, nested local, regional, national, and international scales of the different benefits and goal areas, and their interoperability and interplay across the system of systems.

The Earth Phenomena Observing System: Intelligent Autonomy for Satellite Operations

NASA Technical Reports Server (NTRS)

Ricard, Michael; Abramson, Mark; Carter, David; Kolitz, Stephan

2003-01-01

Earth monitoring systems of the future may include large numbers of inexpensive small satellites, tasked in a coordinated fashion to observe both long term and transient targets. For best performance, a tool which helps operators optimally assign targets to satellites will be required. We present the design of algorithms developed for real-time optimized autonomous planning of large numbers of small single-sensor Earth observation satellites. The algorithms will reduce requirements on the human operators of such a system of satellites, ensure good utilization of system resources, and provide the capability to dynamically respond to temporal terrestrial phenomena. Our initial real-time system model consists of approximately 100 satellites and large number of points of interest on Earth (e.g., hurricanes, volcanoes, and forest fires) with the objective to maximize the total science value of observations over time. Several options for calculating the science value of observations include the following: 1) total observation time, 2) number of observations, and the 3) quality (a function of e.g., sensor type, range, slant angle) of the observations. An integrated approach using integer programming, optimization and astrodynamics is used to calculate optimized observation and sensor tasking plans.

Building a Global Earth Observation System of Systems (GEOSS) and Its Interoperability Challenges

NASA Astrophysics Data System (ADS)

Ryan, B. J.

2015-12-01

Launched in 2005 by industrialized nations, the Group on Earth Observations (GEO) began building the Global Earth Observation System of Systems (GEOSS). Consisting of both a policy framework, and an information infrastructure, GEOSS, was intended to link and/or integrate the multitude of Earth observation systems, primarily operated by its Member Countries and Participating Organizations, so that users could more readily benefit from global information assets for a number of society's key environmental issues. It was recognized that having ready access to observations from multiple systems was a prerequisite for both environmental decision-making, as well as economic development. From the very start, it was also recognized that the shear complexity of the Earth's system cannot be captured by any single observation system, and that a federated, interoperable approach was necessary. While this international effort has met with much success, primarily in advancing broad, open data policies and practices, challenges remain. In 2014 (Geneva, Switzerland) and 2015 (Mexico City, Mexico), Ministers from GEO's Member Countries, including the European Commission, came together to assess progress made during the first decade (2005 to 2015), and approve implementation strategies and mechanisms for the second decade (2016 to 2025), respectively. The approved implementation strategies and mechanisms are intended to advance GEOSS development thereby facilitating the increased uptake of Earth observations for informed decision-making. Clearly there are interoperability challenges that are technological in nature, and several will be discussed in this presentation. There are, however, interoperability challenges that can be better characterized as economic, governmental and/or political in nature, and these will be discussed as well. With the emergence of the Sustainable Development Goals (SDGs), the World Conference on Disaster Risk Reduction (WCDRR), and the United Nations

Current systematic carbon-cycle observations and the need for implementing a policy-relevant carbon observing system

Treesearch

P. Ciais; A. J. Dolman; A. Bombelli; R. Duren; A. Peregon; P. J. Rayner; C. Miller; N. Gobron; G. Kinderman; G. Marland; N. Gruber; F. Chevallier; R. J. Andres; G. Balsamo; L. Bopp; F.-M. Bréon; G. Broquet; R. Dargaville; T. J. Battin; A. Borges; H. Bovensmann; M. Buchwitz; J. Butler; J. G. Canadell; R. B. Cook; R. DeFries; R. Engelen; K. R. Gurney; C. Heinze; M. Heimann; A. Held; M. Henry; B. Law; S. Luyssaert; J. Miller; T. Moriyama; C. Moulin; R. B. Myneni; C. Nussli; M. Obersteiner; D. Ojima; Y. Pan; J.-D. Paris; S. L. Piao; B. Poulter; S. Plummer; S. Quegan; P. Raymond; M. Reichstein; L. Rivier; C. Sabine; D. Schimel; O. Tarasova; R. Valentini; R. Wang; G. van der Werf; D. Wickland; M. Williams; C. Zehner

2014-01-01

A globally integrated carbon observation and analysis system is needed to improve the fundamental understanding of the global carbon cycle, to improve our ability to project future changes, and to verify the effectiveness of policies aiming to reduce greenhouse gas emissions and increase carbon sequestration. Building an integrated carbon observation system requires...

"New Space Explosion" and Earth Observing System Capabilities

NASA Astrophysics Data System (ADS)

Stensaas, G. L.; Casey, K.; Snyder, G. I.; Christopherson, J.

2017-12-01

This presentation will describe recent developments in spaceborne remote sensing, including introduction to some of the increasing number of new firms entering the market, along with new systems and successes from established players, as well as industry consolidation reactions to these developments from communities of users. The information in this presentation will include inputs from the results of the Joint Agency Commercial Imagery Evaluation (JACIE) 2017 Civil Commercial Imagery Evaluation Workshop and the use of the US Geological Survey's Requirements Capabilities and Analysis for Earth Observation (RCA-EO) centralized Earth observing systems database and how system performance parameters are used with user science applications requirements.

Observations of the Pluto-Charon System

NASA Technical Reports Server (NTRS)

Tholen, David J.

2004-01-01

We are continuing the analysis of adaptive optics observations of the Pluto-Charon system, with the goal of confirming the orbital eccentricity reported by Tholen and Bule (1997). Previous work on these data, obtained with the Hokupa's adaptive optics system and Gemini North and reported by Tholea (2002), utilized only a portion of the full set of 348 images taken on 8 nights between 2001 and 2002, and was based on a preliminary calibration of the image scale and position angle of the detector. For each of the three observing runs, independent calibrations were performed using the motion of an asteroid past a fixed stellar source to remove any minor differences in the way the instrument was mounted on the telescope for each run. The image scales determined for each run are good to better than 1 part in 1000, while the individual position angle determinations are good at least 0.1 deg. The preliminary analysis reported at last year's DPS meeting indicated consistency with the orbit determined from the HST observations acquired a decade ago, however, a more careful analysis yields a longitude of periapsis of 132.2 degrees plus or minus 9.3 degrees, disagreeing with the HST results: Finally, possible explanation for the differences in orbital solutions are considered.

Spatial and temporal variation of seismic velocity during earthquakes and volcanic eruptions in western Japan: Insight into mechanism for seismic velocity variation

NASA Astrophysics Data System (ADS)

Tsuji, T.; Ikeda, T.; Nimiya, H.

2017-12-01

We report spatio-temporal variations of seismic velocity around the seismogenic faults in western Japan. We mainly focus on the seismic velocity variation during (1) the 2016 Off-Mie earthquake in the Nankai subduction zone (Mw5.8) and (2) the 2016 Kumamoto earthquake in Kyushu Island (Mw7.0). We applied seismic interferometry and surface wave analysis to the ambient noise data recorded by Hi-net and DONET seismometers of National Research Institute for Earth Science and Disaster Resilience (NIED). Seismic velocity near the rupture faults and volcano decreased during the earthquake. For example, we observed velocity reduction around the seismogenic Futagawa-Hinagu fault system and Mt Aso in the 2016 Kumamoto earthquake. We also identified velocity increase after the eruptions of Mt Aso. During the 2016 Off-Mie earthquake, we observed seismic velocity variation in the Nankai accretionary prism. After the earthquakes, the seismic velocity gradually returned to the pre-earthquake value. The velocity recovering process (healing process) is caused by several mechanisms, such as pore pressure reduction, strain change, and crack sealing. By showing the velocity variations obtained at different geologic settings (volcano, seismogenic fault, unconsolidated sediment), we discuss the mechanism of seismic velocity variation as well as the post-seismic fault healing process.

Microbial arsenic oxidation in a shallow marine hydrothermal vent system

NASA Astrophysics Data System (ADS)

Amend, J. P.; Meyer-Dombard, D. R.; Pichler, T.; Price, R.; Herndon, E.; Hsia, N.

2005-12-01

The toxic effects of arsenic are well documented, but this Group V element can also serve as an energy source to a diverse group of microorganisms. Most of the attention has been on arsenate (AsV) reduction, but the focus is shifting to include arsenite (AsIII) oxidation and subsequent immobilization through coprecipitation with iron (oxy)hydroxides. The shallow marine hydrothermal fluids near Ambitle Island, Papua New Guinea are characterized by arsenite concentrations of up to 1,000 μg/L. Directly proximal to the vent orifices, arsenate coprecipitates with 2-line ferrihydrite, coating rocks and corals in red and green biofilms up to 1 cm thick. DNA extracted from these coatings was amplified with archaeal- and bacterial-specific primers, and the 16S rRNA gene was sequenced. Both biofilm samples revealed archaeal communities exclusively composed of uncultured Crenarchaea. The bacterial members are primarily gamma Proteobacteria and Planctomycetes in the red biofilm, but 60% of the community in the green biofilm affiliate with the alpha Proteobacteria and candidate group OP11; there is minimal overlap in bacterial phylotypes between the two coatings. Slurries from these coatings were also used to inoculate geochemically designed growth media supplemented with various redox couples, including aerobic and anaerobic As(III) oxidation. On a medium targeting anaerobic, chemolithoautotrophic arsenic oxidation coupled to ferric iron reduction at 50 °C, predominantly rod-shaped organisms (~5×105 cells/ml) were enriched. In contrast, on an aerobic arsenic oxidation medium, coccoid-shaped organisms (~3×106 cells/ml) were enriched. The respective thermophilic microbial communities may be taking advantage of overall metabolisms represented by H3AsO3(aq) + 2FeOOH(s) + 3H+ = H2AsO4- + 2Fe2+ + 3H2O (1) and H3AsO3(aq) + 1/2O2(aq) = H2AsO4- + H+. (2) To date, no arsenite oxidizers are known to use ferric iron as a terminal electron acceptor (reaction 1). However, this

The disagreement between the ideal observer and human observers in hardware and software imaging system optimization: theoretical explanations and evidence

NASA Astrophysics Data System (ADS)

He, Xin

2017-03-01

The ideal observer is widely used in imaging system optimization. One practical question remains open: do the ideal and human observers have the same preference in system optimization and evaluation? Based on the ideal observer's mathematical properties proposed by Barrett et. al. and the empirical properties of human observers investigated by Myers et. al., I attempt to pursue the general rules regarding the applicability of the ideal observer in system optimization. Particularly, in software optimization, the ideal observer pursues data conservation while humans pursue data presentation or perception. In hardware optimization, the ideal observer pursues a system with the maximum total information, while humans pursue a system with the maximum selected (e.g., certain frequency bands) information. These different objectives may result in different system optimizations between human and the ideal observers. Thus, an ideal observer optimized system is not necessarily optimal for humans. I cite empirical evidence in search and detection tasks, in hardware and software evaluation, in X-ray CT, pinhole imaging, as well as emission computed tomography to corroborate the claims. (Disclaimer: the views expressed in this work do not necessarily represent those of the FDA)

Tephra Sedimentation from a Short-term Wind-affected Volcanic Plume of the 8 October 2016 Aso Nakadake Eruption, Japan

NASA Astrophysics Data System (ADS)

Tsuji, T.; Nishizaka, N.; Onishi, K.

2017-12-01

Sedimentation processes during explosive volcanic eruptions can be constrained based on detailed analysis of grain-size variation of tephra deposits. Especially, an accurate description of the amount of fine particles has also significant implications for the assessment of specific tephra hazards. Grain size studies for single short-term eruption has advantage to contribute understanding the sedimentation processes because it is simple compared to long-lasting eruption. The 2016 Aso Nakadake eruption, Japan represents an ideal for the study of short-term eruptions thanks to an accurate investigation. Then, we investigate the grain size variation with distance from the vent and sedimentological features of the deposit to discuss the sedimentation processes of the tephra fragments. The eruption provided pyroclastic flow deposit and fallout tephra which distributed NE to ENE direction from the vent. The deposits between 4 and 20 km from vent consist of fine-coated lapilli to coarse ash, ash pellet and mud droplet in ascending degree. The samples are lapilli-bearing within 20 km from vent and those outside of 20 km mainly consist of ash particles. Detailed analyses of individual samples highlight a rapid decay of maximum and mean grain size for the deposit from proximal to distal. The decay trend of maximum grain-size is approximated by three segments of exponential curves with two breaks-in-slope at 10 and 40 km from vent. Most of the sampled deposits are characterized by bimodal grain-size distributions, with the modes of the coarse subpopulation decreasing with distance from vent and those of the fine subpopulation being mostly stable. The fine subpopulation has been interpreted as being mostly associated with size-selective sedimentation processes (e.g., particle aggregation) confirmed by the existence of fine-coated particles, ash pellet and mud droplet. As the fine-coated particles generally have a higher terminal velocity than the individual constituent

Economic value of ecosystem conservation in Japan: Reduction of starting point bias by bid effect function

Treesearch

Mitsuyasu Yabe

2007-01-01

Over 18 million people visit and enjoy the view of the world’s largest class caldera topography, which forms the important landscape element of National Park Aso. Aso grassland spreads and rare plants exist in the harmony of nature and human activities. This study was a Contingent Valuation (CV) survey to estimate the conservation value of Aso grassland. We...

Altimetric system: Earth observing system. Volume 2h: Panel report

NASA Technical Reports Server (NTRS)

Bindschadler, Robert A.; Born, George; Chase, Robert R. P.; Fu, Lee-Lueng; Mouginis-Mark, Peter; Parsons, Chester; Tapley, Byron

1987-01-01

A rationale and recommendations for planning, implementing, and operating an altimetric system aboard the Earth observing system (Eos) spacecraft is provided. In keeping with the recommendations of the Eos Science and Mission Requirements Working Group, a complete altimetric system is defined that is capable of perpetuating the data set to be derived from TOPEX/Poseidon, enabling key scientific questions to be addressed. Since the scientific utility and technical maturity of spaceborne radar altimeters is well documented, the discussion is limited to highlighting those Eos-specific considerations that materially impact upon radar altimetric measurements.

Economic Value of an Advanced Climate Observing System

NASA Astrophysics Data System (ADS)

Wielicki, B. A.; Cooke, R.; Young, D. F.; Mlynczak, M. G.

2013-12-01

Scientific missions increasingly need to show the monetary value of knowledge advances in budget-constrained environments. For example, suppose a climate science mission promises to yield decisive information on the rate of human caused global warming within a shortened time frame. How much should society be willing to pay for this knowledge today? The US interagency memo on the social cost of carbon (SCC) creates a standard yardstick for valuing damages from carbon emissions. We illustrate how value of information (VOI) calculations can be used to monetize the relative value of different climate observations. We follow the SCC, setting uncertainty in climate sensitivity to a truncated Roe and Baker (2007) distribution, setting discount rates of 2.5%, 3% and 5%, and using one of the Integrated Assessment Models sanctioned in SCC (DICE, Nordhaus 2008). We consider three mitigation scenarios: Business as Usual (BAU), a moderate mitigation response DICE Optimal, and a strong response scenario (Stern). To illustrate results, suppose that we are on the BAU emissions scenario, and that we would switch to the Stern emissions path if we learn with 90% confidence that the decadal rate of temperature change reaches or exceeds 0.2 C/decade. Under the SCC assumptions, the year in which this happens, if it happens, depends on the uncertain climate sensitivity and on the emissions path. The year in which we become 90% certain that it happens depends, in addition, on our Earth observations, their accuracy, and their completeness. The basic concept is that more accurate observations can shorten the time for societal decisions. The economic value of the resulting averted damages depends on the discount rate, and the years in which the damages occur. A new climate observation would be economically justified if the net present value (NPV) of the difference in averted damages, relative to the existing systems, exceeds the NPV of the system costs. Our results (Cooke et al. 2013

OBSERVATIONS OF HIERARCHICAL SOLAR-TYPE MULTIPLE STAR SYSTEMS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roberts, Lewis C. Jr.; Tokovinin, Andrei; Mason, Brian D.

2015-10-15

Twenty multiple stellar systems with solar-type primaries were observed at high angular resolution using the PALM-3000 adaptive optics system at the 5 m Hale telescope. The goal was to complement the knowledge of hierarchical multiplicity in the solar neighborhood by confirming recent discoveries by the visible Robo-AO system with new near-infrared observations with PALM-3000. The physical status of most, but not all, of the new pairs is confirmed by photometry in the Ks band and new positional measurements. In addition, we resolved for the first time five close sub-systems: the known astrometric binary in HIP 17129AB, companions to the primariesmore » of HIP 33555, and HIP 118213, and the companions to the secondaries in HIP 25300 and HIP 101430. We place the components on a color–magnitude diagram and discuss each multiple system individually.« less

Molecular Imaging and Pharmacokinetic Analysis of Carbon-11 Labeled Antisense Oligonucleotide LY2181308 in Cancer Patients

PubMed Central

Saleem, Azeem; Matthews, Julian C.; Ranson, Malcolm; Callies, Sophie; André, Valérie; Lahn, Michael; Dickinson, Claire; Prenant, Christian; Brown, Gavin; McMahon, Adam; Talbot, Denis C.; Jones, Terry; Price, Patricia M.

2011-01-01

Antisense oligonucleotides (ASOs) have potential as anti-cancer agents by specifically modulating genes involved in tumorigenesis. However, little is known about ASO biodistribution and tissue pharmacokinetics (PKs) in humans, including whether sufficient delivery to target tumor tissue may be achieved. In this preliminary study in human subjects, we used combined positron emission and computed tomography (PET-CT) imaging and subsequent modeling analysis of acquired dynamic data, to examine the in vivo biodistribution and PK properties of LY2181308 - a second generation ASO which targets the apoptosis inhibitor protein survivin. Following radiolabeling of LY2181308 with methylated carbon-11 ([11C]methylated-LY2181308), micro-doses (<1mg) were administered to three patients with solid tumors enrolled in a phase I trial. Moderate uptake of [11C]methylated-LY2181308 was observed in tumors (mean=32.5ng*h /mL, per mg administered intravenously). Highest uptake was seen in kidney and liver and lowest uptake was seen in lung and muscle. One patient underwent repeat analysis on day 15 of multiple dose therapy, during administration of LY2181308 (750mg), when altered tissue PKs and a favorable change in biodistribution was seen. [11C]methylated-LY2181308 exposure increased in tumor, lung and muscle, whereas renal and hepatic exposure decreased. This suggests that biological barriers to ASO tumor uptake seen at micro-doses were overcome by therapeutic dosing. In addition, 18F-labeled fluorodeoxyglucose (FDG) scans carried out in the same patient before and after treatment showed up to 40% decreased tumor metabolism. For the development of anti-cancer ASOs, the results provide evidence of LY2181308 tumor tissue delivery and add valuable in vivo pharmacological information. For the development of novel therapeutic agents in general, the study exemplifies the merits of applying PET imaging methodology early in clinical investigations. PMID:21772926

NOAO observing proposal processing system

NASA Astrophysics Data System (ADS)

Bell, David J.; Gasson, David; Hartman, Mia

2002-12-01

Since going electronic in 1994, NOAO has continued to refine and enhance its observing proposal handling system. Virtually all related processes are now handled electronically. Members of the astronomical community can submit proposals through email, web form or via Gemini's downloadable Phase-I Tool. NOAO staff can use online interfaces for administrative tasks, technical reviews, telescope scheduling, and compilation of various statistics. In addition, all information relevant to the TAC process is made available online. The system, now known as ANDES, is designed as a thin-client architecture (web pages are now used for almost all database functions) built using open source tools (FreeBSD, Apache, MySQL, Perl, PHP) to process descriptively-marked (LaTeX, XML) proposal documents.

SCHeMA web-based observation data information system

NASA Astrophysics Data System (ADS)

Novellino, Antonio; Benedetti, Giacomo; D'Angelo, Paolo; Confalonieri, Fabio; Massa, Francesco; Povero, Paolo; Tercier-Waeber, Marie-Louise

2016-04-01

It is well recognized that the need of sharing ocean data among non-specialized users is constantly increasing. Initiatives that are built upon international standards will contribute to simplify data processing and dissemination, improve user-accessibility also through web browsers, facilitate the sharing of information across the integrated network of ocean observing systems; and ultimately provide a better understanding of the ocean functioning. The SCHeMA (Integrated in Situ Chemical MApping probe) Project is developing an open and modular sensing solution for autonomous in situ high resolution mapping of a wide range of anthropogenic and natural chemical compounds coupled to master bio-physicochemical parameters (www.schema-ocean.eu). The SCHeMA web system is designed to ensure user-friendly data discovery, access and download as well as interoperability with other projects through a dedicated interface that implements the Global Earth Observation System of Systems - Common Infrastructure (GCI) recommendations and the international Open Geospatial Consortium - Sensor Web Enablement (OGC-SWE) standards. This approach will insure data accessibility in compliance with major European Directives and recommendations. Being modular, the system allows the plug-and-play of commercially available probes as well as new sensor probess under development within the project. The access to the network of monitoring probes is provided via a web-based system interface that, being implemented as a SOS (Sensor Observation Service), is providing standard interoperability and access tosensor observations systems through O&M standard - as well as sensor descriptions - encoded in Sensor Model Language (SensorML). The use of common vocabularies in all metadatabases and data formats, to describe data in an already harmonized and common standard is a prerequisite towards consistency and interoperability. Therefore, the SCHeMA SOS has adopted the SeaVox common vocabularies populated by

Big Data in the Earth Observing System Data and Information System

NASA Technical Reports Server (NTRS)

Lynnes, Chris; Baynes, Katie; McInerney, Mark

2016-01-01

Approaches that are being pursued for the Earth Observing System Data and Information System (EOSDIS) data system to address the challenges of Big Data were presented to the NASA Big Data Task Force. Cloud prototypes are underway to tackle the volume challenge of Big Data. However, advances in computer hardware or cloud won't help (much) with variety. Rather, interoperability standards, conventions, and community engagement are the key to addressing variety.

Earth Observing System (EOS) advanced altimetry

NASA Technical Reports Server (NTRS)

Parsons, C. L.; Walsh, E. J.

1988-01-01

In the post-TOPEX era, satellite radar altimeters will be developed with the capability of measuring the earth's surface topography over a wide swath of coverage, rather than just at the satellite's nadir. The identification of potential spacecraft flight missions in the future was studied. The best opportunity was found to be the Earth Observing System (EOS). It is felt that an instrument system that has a broad appeal to the earth sciences community stands a much better chance of being selected as an EOS instrument. Consequently, the Topography and Rain Radar Imager (TARRI) will be proposed as a system that has the capability to profile the Earth's topography regardless of the surface type. The horizontal and height resolutions of interest are obviously significantly different over land, ice, and water; but, the use of radar to provide an all-weather observation capability is applicable to the whole earth. The scientific guidance for the design and development of this instrument and the eventual scientific utilization of the data produced by the TARRI will be provided by seven science teams. The teams are formed around scientific disciplines and are titled: Geology/Geophysics, Hydrology/Rain, Oceanography, Ice/Snow, Geodesy/Orbit/Attitude, Cartography, and Surface Properties/Techniques.

INTAROS: Development of an integrated Arctic observation system under Horizon 2020

NASA Astrophysics Data System (ADS)

Beszczynska-Möller, Agnieszka; Sandven, Stein; Sagen, Hanne

2017-04-01

INTAROS is a research and innovation action funded under the H2020-BG-09 call for the five-year period 2016-2021. INTAROS will develop an integrated Arctic Observation System (iAOS) by extending, improving and unifying existing systems in the different regions of the Arctic. INTAROS will have a strong multidisciplinary focus, with tools for integration of data from atmosphere, ocean, cryosphere and terrestrial sciences, provided by institutions in Europe, North America and Asia. Satellite earth observation (EO) data plays an increasingly important role in such observing systems, because the amount of EO data for observing the global climate and environment grows year by year. EO data will therefore be integrated into iAOS based on existing products and databases. In situ observing systems are much more limited due to logistical constraints and cost limitations. The sparseness of in situ data is therefore the largest gap in the overall observing system. INTAROS will assess strengths and weaknesses of existing Arctic observing systems and contribute with innovative solutions to fill some of the critical gaps in the selected networks. INTAROS will develop a platform, iAOS, to search for and access data from distributed databases. The evolution into a sustainable Arctic observing system requires coordination, mobilization and cooperation between the existing European and international infrastructures (in-situ and remote, including space-based), the modeling communities and relevant stakeholder groups. INTAROS will include development of community-based observing systems, where local knowledge is merged with scientific data. Multidisciplinary data integrated under INTAROS will contribute to better understanding of interactions and coupling in the complex Arctic ice-ocean-land-atmosphere system. An integrated Arctic Observation System will enable better-informed decisions and better-documented processes within key sectors (e.g. local communities, shipping, tourism

SEU induced errors observed in microprocessor systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Asenek, V.; Underwood, C.; Oldfield, M.

In this paper, the authors present software tools for predicting the rate and nature of observable SEU induced errors in microprocessor systems. These tools are built around a commercial microprocessor simulator and are used to analyze real satellite application systems. Results obtained from simulating the nature of SEU induced errors are shown to correlate with ground-based radiation test data.

Metabolic alterations and neurodevelopmental outcome of infants with transposition of the great arteries.

PubMed

Park, I Sook; Yoon, S Young; Min, J Yeon; Kim, Y Hwue; Ko, J Kok; Kim, K Soo; Seo, D Man; Lee, J Hee

2006-01-01

Abnormal neurodevelopment has been reported for infants who were born with transposition of the great arteries (TGA) and underwent arterial switch operation (ASO). This study evaluates the cerebral metabolism of TGA infants at birth and before ASO and neurodevelopment 1 year after ASO. Proton magnetic resonance spectroscopy (1H-MRS) was performed on 16 full-term TGA brains before ASO within 3-6 days after birth. The brain metabolite ratios of [NAA/Cr], [Cho/Cr], and [mI/Cr] evaluated measured. Ten infants were evaluated at 1 year using the Bayley Scales of Infants Development II (BSED II). Cerebral metabolism of infants with TGA was altered in parietal white matter (PWM) and occipital gray matter (OGM) at birth before ASO. One year after ASO, [Cho/Cr] in PWM remained altered, but all metabolic ratios in OGM were normal. The results of BSID II at 1 year showed delayed mental and psychomotor development. This delayed neurodevelopmental outcome may reflect consequences of the altered cerebral metabolism in PWM measured by 1H-MRS. It is speculated that the abnormal hemodynamics due to TGA in utero may be responsible for the impaired cerebral metabolism and the subsequent neurodevelopmental deficit.

Effects of Different Observational Systems and Time Sequences Upon Non-Participant Observers' Behavioral Ratings.

ERIC Educational Resources Information Center

Wodarski, John S.; And Others

Four different observational systems and two time sequences were employed to determine the extent to which they would yield different incidences of anti-social behavior. Two videotapes, randomly chosen from a pool of 30 tapes, were utilized. These illustrated the behaviors of anti-social children in a natural setting. Six observers were reliably…

Transonic Compressor: Program System TXCO for Data Acquisition and On-Line Reduction.

DTIC Science & Technology

1980-10-01

IMONIDAYIYEARIHOUR,IMINISEC) OS16 C ............................................................... (0S17 C 0SiB C Gel dole ond line and convert the...linear curve fits SECON real intercept of linear curve fit (as from CURVE) 65 - . FLOW CHART SUBROUTINE CALIB - - - Aso C’A / oonre& *Go wSAt*irc

The Central Nucleus of the Amygdala and Corticotropin-Releasing Factor: Insights into Contextual Fear Memory

PubMed Central

Pitts, Matthew W.; Todorovic, Cedomir; Blank, Thomas; Takahashi, Lorey K.

2009-01-01

The central nucleus of the amygdala (CeA) has been traditionally viewed in fear conditioning to serve as an output neural center that transfers conditioned information formed in the basolateral amygdala to brain structures that generate emotional responses. Recent studies suggest that the CeA may also be involved in fear memory consolidation. In addition, corticotropin-releasing factor systems were shown to facilitate memory consolidation in the amygdala, which contains a high density of CRF immunoreactive cell bodies and fibers in the lateral part of the CeA (CeAl). However, the involvement of CeA CRF in contextual fear conditioning remains poorly understood. Therefore, we first conducted a series of studies using fiber-sparing lesion and reversible inactivation methods to assess the general role of the CeA in contextual fear. We then used identical training and testing procedures to compare and evaluate the specific function of CeA CRF using CRF antisense oligonucleotides (CRF ASO). Rats microinjected with ibotenic acid, muscimol, or a CRF ASO into the CeA prior to contextual fear conditioning showed typical levels of freezing during acquisition training but exhibited significant reductions in contextual freezing in a retention test 48 h later. Furthermore, CeA inactivation induced by either muscimol or CRF ASO administration immediately prior to retention testing did not impair freezing, suggesting that the previously observed retention deficits were due to inhibition of consolidation rather than fear expression. Collectively, our results suggest CeA involvement in the consolidation of contextual fear memory and specifically implicate CeA CRF as an important mediator. PMID:19494159

Linear system identification via backward-time observer models

NASA Technical Reports Server (NTRS)

Juang, Jer-Nan; Phan, Minh Q.

1992-01-01

Presented here is an algorithm to compute the Markov parameters of a backward-time observer for a backward-time model from experimental input and output data. The backward-time observer Markov parameters are decomposed to obtain the backward-time system Markov parameters (backward-time pulse response samples) for the backward-time system identification. The identified backward-time system Markov parameters are used in the Eigensystem Realization Algorithm to identify a backward-time state-space model, which can be easily converted to the usual forward-time representation. If one reverses time in the model to be identified, what were damped true system modes become modes with negative damping, growing as the reversed time increases. On the other hand, the noise modes in the identification still maintain the property that they are stable. The shift from positive damping to negative damping of the true system modes allows one to distinguish these modes from noise modes. Experimental results are given to illustrate when and to what extent this concept works.

Paleomagnetic Directions of 3-4ka Basaltic Volcanoes in the Aso Central Cone, Kyushu Japan: Contributions to the Paleosecular Variation and the Volcano-Stratigraphic Studies.

NASA Astrophysics Data System (ADS)

Shibuya, H.; Mochizuki, N.; Miyabuchi, Y.

2017-12-01

In the central cone of Aso volcano, Kyushu Japan, there are 4 basaltic volcanic cones of 3-4 ka in age. The lava flows from those cones spread on the flank of the cones, and they were classified in the relation to each cone. The composition and lithology of those lavas are, however, often difficult to distinguish each other. Thus, we try the magnetostratigraphic study of those lava flows to confirm the classification. The samples were collected from 22 sites, one from a scoria cone and others are from lava, and measured their paleomagnetism. The magnetization of those samples is quite simple, as expected, and alternating field demagnetization well defines the primary component. The site mean directions aligns well on an arc, which defines the paleosecular variation of those ages, 3-4ka. The lava flows and a welded scoria classified as of two centers (Komezuka and Kamikomezuka) are well clustered and confirmed to a single or very closely erupted in time for each center. On the other hand, lava flows related to the other two centers (Ojo and Kijima) have multiple clusters in paleomagnetic directions, and their ages estimated from the paleosecular variation curve interfingers to the classification. It is also very interesting that there seems to be a stagnant point in secular variation just before 3ka, whose direction is similar to the known stagnant point in archeomagnetic secular variation at around 800CE. If there is tendency to stop the SV at the direction, it may be related to the core dynamo processes.

(TES) Thermal Enclosure System with (COS) Crystal Observation System

NASA Technical Reports Server (NTRS)

1993-01-01

The COS consists of a specially designed (VDA) Vapor Diffusion Apparatus tray with 6 chambers, a video camera for each chamber, a lighting system, and associated hardware. By observing the crystal growth in each chamber, researchers can identify which conditions and concentrations of proteins and precipitants are best for promoting the crystal growth to a particular protein.

The Jupiter System Observer: Probing the Foundations of Planetary Systems

NASA Astrophysics Data System (ADS)

Senske, D.; Prockter, L.; Collins, G.; Cooper, J.; Hendrix, A.; Hibbitts, K.; Kivelson, M.; Orton, G.; Schubert, G.; Showman, A.; Turtle, E.; Williams, D.; Kwok, J.; Spilker, T.; Tan-Wang, G.

2007-12-01

Galileo's observations in the 1600's of the dynamic system of Jupiter and its moons launched a revolution in understanding the way planetary systems operate. Now, some 400 years later, the discovery of extra solar planetary systems with Jupiter-sized bodies has led to a similar revolution in thought regarding how these systems form and evolve. From the time of Galileo, the Jovian system has been viewed as a solar system in miniature, providing a laboratory to study, diverse and dynamic processes in a single place. The icy Galilean satellites provide a window into solar system history by preserving in their cratering records a chronology dating back nearly 4.5 By and extending to the present. The continuously erupting volcanoes of Io may provide insight into the era when magma oceans were common. The discovery of an internally generated magnetic field at Ganymede, one of only three terrestrial bodies to possess such a field, is a place to gain insight as to how dynamos work. The confirmation and characterization of icy satellite subsurface oceans impacts the way habitability is considered. Understanding the composition and volatile inventory of Jupiter can shed light into how planets accrete from the solar nebulae. Finally, like our sun, Jupiter influences its system through its extensive magnetic field. In early 2007, NASA's Science Mission Directorate formed four Science Definition Teams (SDTs) to formulate science goals and objectives in anticipation of the initiation of a flagship-class mission to the outer solar system (Europa, Jupiter system, Titan and Enceladus). The Jupiter System Observer (JSO) mission concept emphasizes overall Jupiter system science: 1) Jupiter and its atmosphere, 2) the geology and geophysics of the Galilean satellites (Io, Europa, Ganymede and Callisto), 3) the magnetosphere environment - both Jupiter's and Ganymede's&pand 4) interactions within the system. Focusing on the unique geology, presence of an internal magnetic field and

System study of the carbon dioxide observational platform system (CO-OPS): Project overview

NASA Technical Reports Server (NTRS)

Stephens, J. Briscoe; Thompson, Wilbur E.

1987-01-01

The resulting options from a system study for a near-space, geo-stationary, observational monitoring platform system for use in the Department of Energy's (DOE) National Carbon Dioxide Observational Platform System (CO-OPS) on the greenhouse effect are discussed. CO-OPS is being designed to operate continuously for periods of up to 3 months in quasi-fixed position over most global regional targets of interest and could make horizon observations over a land-sea area of circular diameter up to about 600 to 800 statute miles. This affords the scientific and engineering community a low-cost means of operating their payloads for monitoring the regional parameters they deem relevant to their investigations of the carbon dioxide greenhouse effect at one-tenth the cost of most currently utilized comparable remote sensing techniques.

AtlantOS - Optimizing and Enhancing the Integrated Atlantic Ocean Observing System

NASA Astrophysics Data System (ADS)

Reitz, Anja; Visbeck, Martin; AtlantOS Consortium, the

2016-04-01

Atlantic Ocean observation is currently undertaken through loosely-coordinated, in-situ observing networks, satellite observations and data management arrangements of heterogeneous international, national and regional design to support science and a wide range of information products. Thus there is tremendous opportunity to develop the systems towards a fully integrated Atlantic Ocean Observing System consistent with the recently developed 'Framework of Ocean Observing'. The vision of AtlantOS is to improve and innovate Atlantic observing by using the Framework of Ocean Observing to obtain an international, more sustainable, more efficient, more integrated, and fit-for-purpose system. Hence, the AtlantOS initiative will have a long-lasting and sustainable contribution to the societal, economic and scientific benefit arising from this integrated approach. This will be delivered by improving the value for money, extent, completeness, quality and ease of access to Atlantic Ocean data required by industries, product supplying agencies, scientist and citizens. The overarching target of the AtlantOS initiative is to deliver an advanced framework for the development of an integrated Atlantic Ocean Observing System that goes beyond the state-of -the-art, and leaves a legacy of sustainability after the life of the project. The legacy will derive from the following aims: i) to improve international collaboration in the design, implementation and benefit sharing of ocean observing, ii) to promote engagement and innovation in all aspects of ocean observing, iii) to facilitate free and open access to ocean data and information, iv) to enable and disseminate methods of achieving quality and authority of ocean information, v) to strengthen the Global Ocean Observing System (GOOS) and to sustain observing systems that are critical for the Copernicus Marine Environment Monitoring Service and its applications and vi) to contribute to the aims of the Galway Statement on Atlantic

The Effects of 2′-O-Methoxyethyl Containing Antisense Oligonucleotides on Platelets in Human Clinical Trials

PubMed Central

Baker, Brenda F.; Witztum, Joseph L.; Kwoh, T. Jesse; Pham, Nguyen C.; Salgado, Nelson; McEvoy, Bradley W.; Cheng, Wei; Hughes, Steven G.; Bhanot, Sanjay; Geary, Richard S.

2017-01-01

A thorough analysis of clinical trial data in the Ionis integrated safety database (ISDB) was performed to determine if there is a class effect on platelet numbers and function in subjects treated with 2′-O-methoxyethyl (2′MOE)-modified antisense oligonucleotides (ASOs). The Ionis ISDB includes over 2,600 human subjects treated with 16 different 2′MOE ASOs in placebo-controlled and open-label clinical trials over a range of doses up to 624 mg/week and treatment durations as long as 4.6 years. This analysis showed that there is no class generic effect on platelet numbers and no incidence of confirmed platelet levels below 50 K/μL in subjects treated with 2′MOE ASOs. Only 7 of 2,638 (0.3%) subjects treated with a 2′MOE ASO experienced a confirmed postbaseline (BSLN) platelet count between 100 and 50 K/μL. Three of sixteen 2′MOE ASOs had >10% incidence of platelet decreases >30% from BSLN, suggesting that certain sequences may associate with clinically insignificant platelet declines. Further to these results, we found no evidence that 2′MOE ASOs alter platelet function, as measured by the lack of clinically relevant bleeding in the presence or absence of other drugs that alter platelet function and/or number and by the results from trials conducted with the factor XI (FXI) ASO. PMID:28145801

Demonstrating the Alaska Ocean Observing System in Prince William Sound

NASA Astrophysics Data System (ADS)

Schoch, G. Carl; McCammon, Molly

2013-07-01

The Alaska Ocean Observing System and the Oil Spill Recovery Institute developed a demonstration project over a 5 year period in Prince William Sound. The primary goal was to develop a quasi-operational system that delivers weather and ocean information in near real time to diverse user communities. This observing system now consists of atmospheric and oceanic sensors, and a new generation of computer models to numerically simulate and forecast weather, waves, and ocean circulation. A state of the art data management system provides access to these products from one internet portal at http://www.aoos.org. The project culminated in a 2009 field experiment that evaluated the observing system and performance of the model forecasts. Observations from terrestrial weather stations and weather buoys validated atmospheric circulation forecasts. Observations from wave gages on weather buoys validated forecasts of significant wave heights and periods. There was an emphasis on validation of surface currents forecasted by the ocean circulation model for oil spill response and search and rescue applications. During the 18 day field experiment a radar array mapped surface currents and drifting buoys were deployed. Hydrographic profiles at fixed stations, and by autonomous vehicles along transects, were made to acquire measurements through the water column. Terrestrial weather stations were the most reliable and least costly to operate, and in situ ocean sensors were more costly and considerably less reliable. The radar surface current mappers were the least reliable and most costly but provided the assimilation and validation data that most improved ocean circulation forecasts. We describe the setting of Prince William Sound and the various observational platforms and forecast models of the observing system, and discuss recommendations for future development.

Atrial Function after the Atrial Switch Operation for Transposition of the Great Arteries: Comparison with Arterial Switch and Normals by Cardiovascular Magnetic Resonance.

PubMed

Franzoso, Francesca D; Wohlmuth, Christoph; Greutmann, Matthias; Kellenberger, Christian J; Oxenius, Angela; Voser, Eva M; Valsangiacomo Buechel, Emanuela R

2016-09-01

The atria serve as reservoir, conduit, and active pump for ventricular filling. The performance of the atrial baffles after atrial switch repair for transposition of the great arteries may be abnormal and impact the function of the systemic right ventricle. We sought to assess atrial function in patients after atrial repair in comparison to patients after arterial switch repair (ASO) and to controls. Using magnetic resonance imaging, atrial volumes and functional parameters were measured in 17 patients after atrial switch repair, 9 patients after ASO and 10 healthy subjects. After the atrial switch operation, the maximum volume of the pulmonary venous atrium was significantly enlarged, but not of the systemic venous atrium. In both patients groups, independently from the surgical technique used, the minimum atrial volumes were elevated, which resulted in a decreased total empting fraction compared with controls (P < .01). The passive empting volume was diminished for right atrium, but elevated for left atrium after atrial switch and normal for left atrium after ASO; however, the passive empting fraction was diminished for both right atrium and left atrium after both operations (P < .01). The active empting volume was the most affected parameter in both atria and both groups and active empting fractions were highly significantly reduced compared with controls. Atrial function is abnormal in all patients, after atrial switch and ASO repair. The cyclic volume changes, that is, atrial filling and empting, are reduced when compared with normal subjects. Thus, the atria have lost part of their capacity to convert continuous venous flow into a pulsatile ventricular filling. The function of the pulmonary venous atrium, acting as preload for the systemic right ventricle, after atrial switch is altered the most. © 2015 Wiley Periodicals, Inc.

Expert systems tools for Hubble Space Telescope observation scheduling

NASA Technical Reports Server (NTRS)

Miller, Glenn; Rosenthal, Don; Cohen, William; Johnston, Mark

1987-01-01

The utility of expert systems techniques for the Hubble Space Telescope (HST) planning and scheduling is discussed and a plan for development of expert system tools which will augment the existing ground system is described. Additional capabilities provided by these tools will include graphics-oriented plan evaluation, long-range analysis of the observation pool, analysis of optimal scheduling time intervals, constructing sequences of spacecraft activities which minimize operational overhead, and optimization of linkages between observations. Initial prototyping of a scheduler used the Automated Reasoning Tool running on a LISP workstation.

78 FR 48080 - Proposed Establishment of Class E Airspace; Magee, MS

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-07

...) Global Positioning System (GPS) Standard Instrument Approach Procedure (SIAP) serving Magee Municipal...) operations within the National Airspace System. DATES: Comments must be received on or before September 23... No. 13-ASO-8) and be submitted in triplicate to the Docket Management System (see ADDRESSES section...

The Earth Observing System Terra Mission

NASA Technical Reports Server (NTRS)

Kaufman, Yoram J.

2000-01-01

Langley's remarkable solar and lunar spectra collected from Mt. Whitney inspired Arrhenius to develop the first quantitative climate model in 1896. After the launch in Dec. 16 1999, NASA's Earth Observing AM Satellite (EOS-Terra) will repeat Langley's experiment, but for the entire planet, thus pioneering a wide array of calibrated spectral observations from space of the Earth System. Conceived in response to real environmental problems, EOS-Terra, in conjunction with other international satellite efforts, will fill a major gap in current efforts by providing quantitative global data sets with a resolution smaller than 1 km on the physical, chemical and biological elements of the earth system. Thus, like Langley's data, EOS-Terra can revolutionize climate research by inspiring a new generation of climate system models and enable us to assess the human impact on the environment. In the talk I shall review the historical perspective of the Terra mission and the key new elements of the mission. We expect to have some first images that demonstrate the most innovative capability from EOS Terra: MODIS - 1.37 microns cirrus channel; 250 m daily cover for clouds and vegetation change; 7 solar channels for land and aerosol; new fire channels; Chlorophyll fluorescence; MISR - 9 multi angle views of clouds and vegetation; MOPITT - Global CO maps and CH4 maps; ASTER - Thermal channels for geological studies with 15-90 m resolution.

An Automated Cloud Observation System (ACOS).

DTIC Science & Technology

1980-12-17

IQ 4. TITLE (and SubliIII.J 5- TYPE OF REPORT & PERIOD COVERED AN.JUTOXMATrD A LOUD OBSERVATION Scientific . Interim. SYSTEM (ALLIS) 6 PERFORMING ORG...p oci -dIi IV ( ( )S). . hhe 6 lists the percentage of agreement realizied by -:~ tc thc fiftt en metthods (I’ p touping ceilomieter t’ ata (see Ta

Using high resolution Lidar data from SnowEx to characterize the sensitivity of snow depth retrievals to point-cloud density and vegetation

NASA Astrophysics Data System (ADS)

Patterson, V. M.; Bormann, K.; Deems, J. S.; Painter, T. H.

2017-12-01

The NASA SnowEx campaign conducted in 2016 and 2017 provides a rich source of high-resolution Lidar data from JPL's Airborne Snow Observatory (ASO - http://aso.jpl.nasa.gov) combined with extensive in-situ measurements in two key areas in Colorado: Grand Mesa and Senator Beck. While the uncertainty in the 50m snow depth retrievals from NASA's ASO been estimated at 1-2cm in non-vegetated exposed areas (Painter et al., 2016), the impact of forest cover and point-cloud density on ASO snow lidar depth retrievals is relatively unknown. Dense forest canopies are known to reduce lidar penetration and ground strikes thus affecting the elevation surface retrieved from in the forest. Using high-resolution lidar point cloud data from the ASO SnowEx campaigns (26pt/m2) we applied a series of data decimations (up to 90% point reduction) to the point cloud data to quantify the relationship between vegetation, ground point density, resulting snow-off and snow-on surface elevations and finally snow depth. We observed non-linear reductions in lidar ground point density in forested areas that were strongly correlated to structural forest cover metrics. Previously, the impacts of these data decimations on a small study area in Grand Mesa showed a sharp increase in under-canopy surface elevation errors of -0.18m when ground point densities were reduced to 1.5pt/m2. In this study, we expanded the evaluation to the more topographically challenging Senator Beck basin, have conducted analysis along a vegetation gradient and are considering snow the impacts of snow depth rather than snow-off surface elevation. Preliminary analysis suggest that snow depth retrievals inferred from airborne lidar elevation differentials may systematically underestimate snow depth in forests where canopy density exceeds 1.75 and where tree heights exceed 5m. These results provide a basis from which to identify areas that may suffer from vegetation-induced biases in surface elevation models and snow depths

Early and progressive sensorimotor anomalies in mice overexpressing wild-type human alpha-synuclein.

PubMed

Fleming, Sheila M; Salcedo, Jonathan; Fernagut, Pierre-Olivier; Rockenstein, Edward; Masliah, Eliezer; Levine, Michael S; Chesselet, Marie-Françoise

2004-10-20

Accumulation of alpha-synuclein in brain is a hallmark of synucleinopathies, neurodegenerative diseases that include Parkinson's disease. Mice overexpressing alpha-synuclein under the Thy-1 promoter (ASO) show abnormal accumulation of alpha-synuclein in cortical and subcortical regions of the brain, including the substantia nigra. We examined the motor deficits in ASO mice with a battery of sensorimotor tests that are sensitive to alterations in the nigrostriatal dopaminergic system. Male wild-type and ASO mice were tested every 2 months for 8 months for motor performance and coordination on a challenging beam, inverted grid, and pole, sensorimotor deficits in an adhesive removal test, spontaneous activity in a cylinder, and gait. Fine motor skills were assessed by the ability to grasp cotton from a bin. ASO mice displayed significant impairments in motor performance and coordination and a reduction in spontaneous activity as early as 2 months of age. Motor performance and coordination impairments became progressively worse with age and sensorimotor deficits appeared at 6 months. Fine motor skills were altered at 4 months and worsened at 8 months. These data indicate that overexpression of alpha-synuclein induced an early and progressive behavioral phenotype that can be detected in multiple tests of sensorimotor function. These behavioral deficits provide a useful way to assess novel drug therapy in genetic models of synucleinopathies.

X-ray Photoelectron Spectroscopic and Raman microscopic investigation of the variscite group minerals: Variscite, strengite, scorodite and mansfieldite

NASA Astrophysics Data System (ADS)

Kloprogge, J. Theo; Wood, Barry J.

2017-10-01

Several structurally related AsO4 and PO4 minerals, were studied with Raman microscopy and X-ray Photoelectron Spectroscopy (XPS). XPS revealed only Fe, As and O for scorodite. The Fe 2p, As 3d, and O 1s indicated one position for Fe2 +, while 2 different environments for O and As were observed. The O 1s at 530.3 eV and the As 3d 5/2 at 43.7 eV belonged to AsO4, while minor bands for O 1s at 531.3 eV and As 3d 5/2 at 44.8 eV were due to AsO4 groups exposed on the surface possibly forming OH-groups. Mansfieldite showed, besides Al, As and O, a trace of Co. The PO4 equivalent of mansfieldite is variscite. The change in crystal structure replacing As with P resulted in an increase in the binding energy (BE) of the Al 2p by 2.9 eV. The substitution of Fe3 + for Al3 + in the structure of strengite resulted in a Fe 2p at 710.8 eV. An increase in the Fe 2p BE of 4.8 eV was found between mansfieldite and strengite. The scorodite Raman OH-stretching region showed a sharp band at 3513 cm- 1 and a broad band around 3082 cm- 1. The spectrum of mansfieldite was like that of scorodite with a sharp band at 3536 cm- 1 and broader maxima at 3100 cm- 1 and 2888 cm- 1. Substituting Al in the arsenate structure instead of Fe resulted in a shift of the metal-OH-stretching mode by 23 cm- 1 towards higher wavenumbers due to a slightly longer H-bonding in mansfieldite compared to scorodite. The intense band for scorodite at 805 cm- 1 was ascribed to the symmetric stretching mode of the AsO4. The medium intensity bands at 890, 869, and 830 cm- 1 were ascribed to the internal modes. A significant shift towards higher wavenumbers was observed for mansfieldite. The strengite Raman spectrum in the 900-1150 cm- 1 shows a strong band at 981 cm- 1 accompanied by a series of less intense bands. The 981 cm- 1 band was assigned to the PO4 symmetric stretching mode, while the weak band at 1116 cm- 1 was the corresponding antisymmetric stretching mode. The remaining bands at 1009, 1023 and 1035 cm- 1

Penn State Radar Systems: Implementation and Observations

NASA Astrophysics Data System (ADS)

Urbina, J. V.; Seal, R.; Sorbello, R.; Kuyeng, K.; Dyrud, L. P.

2014-12-01

Software Defined Radio/Radar (SDR) platforms have become increasingly popular as researchers, hobbyists, and military seek more efficient and cost-effective means for radar construction and operation. SDR platforms, by definition, utilize a software-based interface for configuration in contrast to traditional, hard-wired platforms. In an effort to provide new and improved radar sensing capabilities, Penn State has been developing advanced instruments and technologies for future radars, with primary objectives of making such instruments more capable, portable, and more cost effective. This paper will describe the design and implementation of two low-cost radar systems and their deployment in ionospheric research at both low and mid-latitudes. One radar has been installed near Penn State campus, University Park, Pennsylvania (77.97°W, 40.70°N), to make continuous meteor observations and mid-latitude plasma irregularities. The second radar is being installed in Huancayo (12.05°S, -75.33°E), Peru, which is capable of detecting E and F region plasma irregularities as well as meteor reflections. In this paper, we examine and compare the diurnal and seasonal variability of specular, non- specular, and head-echoes collected with these two new radar systems and discuss sampling biases of each meteor observation technique. We report our current efforts to validate and calibrate these radar systems with other VHF radars such as Jicamarca and SOUSY. We also present the general characteristics of continuous measurements of E-region and F-region coherent echoes using these modern radar systems and compare them with coherent radar events observed at other geographic mid-latitude radar stations.

PROMOTING CANCER PREVENTION AND CONTROL IN COMMUNITY-BASED HIV/AIDS SERVICE ORGANIZATIONS: ARE THEY READY?

PubMed Central

Guidry, John A.; Lubetkin, Erica I.; Corner, Geoffrey W.; Lord-Bessen, Jennifer; Kornegay, Mark; Burkhalter, Jack E.

2015-01-01

Community-based organizations (CBOs) serving persons living with HIV or AIDS face the challenge of an aging population with more chronic diseases. This study assessed cancer programming needs of AIDS service organizations (ASOs) in New York, New Jersey, and Connecticut by conducting a community needs assessment. Sixty (58%) of 103 organizations completed the survey. ASOs conduct activities most related to early steps along the cancer care continuum, but they also express great interest in expanding cancer-focused programming into new areas. ASOs have resources or capacities in assisting HIV+ clients with mental health or substance abuse problems, but there exists a need for funding in undertaking or expanding cancer-focused programs. ASOs are receptive to collaborating with researchers on disseminating cancer prevention and control knowledge in their settings. Community-academic research partnerships enable resonant training and technical assistance methods to be explored that will enhance the abilities of ASOs to bring cancer-related programming to their clients. PMID:24450277

Promoting cancer prevention and control in community-based HIV/AIDS service organizations: are they ready?

PubMed

Guidry, John A; Lubetkin, Erica; Corner, Geoffrey; Lord-Bessen, Jennifer; Kornegay, Mark; Burkhalter, Jack E

2014-02-01

Community-based organizations (CBOs) serving persons living with HIV or AIDS face the challenge of an aging population with more chronic diseases. This study assessed cancer programming needs of AIDS service organizations (ASOs) in New York, New Jersey, and Connecticut by conducting a community needs assessment. Sixty (58%) of 103 organizations completed the survey. ASOs conduct activities most related to early steps along the cancer care continuum, but they also express great interest in expanding cancer-focused programming into new areas. ASOs have resources or capacities in assisting HIV+ clients with mental health or substance abuse problems, but there exists a need for funding in undertaking or expanding cancer-focused programs. ASOs are receptive to collaborating with researchers on disseminating cancer prevention and control knowledge in their settings. Community-academic research partnerships enable resonant training and technical assistance methods to be explored that will enhance the abilities of ASOs to bring cancer-related programming to their clients.

Earth Observing System: Science Objectives and Challenges

NASA Technical Reports Server (NTRS)

King, Michael D.

1999-01-01

The Earth Observing System (EOS) is a space-based observing system comprised of a series of satellite sensors by which scientists can monitor the Earth, a Data and Information System (EOSDIS) enabling researchers worldwide to access the satellite data, and an interdisciplinary science research program to interpret the satellite data. In this presentation we review the key areas of scientific uncertainty in understanding climate and global change, and follow that with a description of the EOS goals, objectives, and scientific research elements that comprise the program (instrument science teams and interdisciplinary investigations). Finally, I will describe how scientists and policy makers intend to use EOS data improve our understanding of key global change uncertainties, such as: (i) clouds and radiation, including fossil fuel and natural emissions of sulfate aerosol and its potential impact on cloud feedback, (ii) man's impact on ozone depletion, with examples of ClO and O3 obtained from the UARS satellite during the Austral Spring, and (iii) volcanic eruptions and their impact on climate, with examples from the eruption of Mt. Pinatubo.

Earth Observing System: Science Objectives and Challenges

NASA Technical Reports Server (NTRS)

King, Michael D.

1998-01-01

The Earth Observing System (EOS) is a space-based observing system comprised of a series of satellite sensors by which scientists can monitor the Earth, a Data and Information System (EOSDIS) enabling researchers worldwide to access the satellite data, and an interdisciplinary science research program to interpret the satellite data. In this presentation I will describe the key areas of scientific uncertainty in understanding climate and global change, and follow that with a description of the EOS goals, objectives, and scientific research elements that comprise the program (instrument science teams and interdisciplinary investigations). Finally, I will describe how scientists and policy makers intend to use EOS data to improve our understanding of key global change uncertainties, such as: (i) clouds and radiation, including fossil fuel and natural emissions of sulfate aerosol and its potential impact on cloud feedback, (ii) man's impact on ozone depletion, with examples of ClO and O3 obtained from the UARS satellite during the Austral Spring, and (iii) volcanic eruptions and their impact on climate, with examples from the eruption of Mt. Pinatubo.

Earth Observing Data System Data and Information System (EOSDIS) Overview

NASA Technical Reports Server (NTRS)

Klene, Stephan

2016-01-01

The National Aeronautics and Space Administration (NASA) acquires and distributes an abundance of Earth science data on a daily basis to a diverse user community worldwide. The NASA Big Earth Data Initiative (BEDI) is an effort to make the acquired science data more discoverable, accessible, and usable. This presentation will provide a brief introduction to the Earth Observing System Data and Information System (EOSDIS) project and the nature of advances that have been made by BEDI to other Federal Users.

Observation and integrated Earth-system science: A roadmap for 2016–2025

DOE Office of Scientific and Technical Information (OSTI.GOV)

Simmons, Adrian; Fellous, Jean-Louis; Ramaswamy, V.

This report is the response to a request by the Committee on Space Research of the International Council for Science to prepare a roadmap on observation and integrated Earth-system science for the coming ten years. Its focus is on the combined use of observations and modelling to address the functioning, predictability and projected evolution of interacting components of the Earth system on timescales out to a century or so. It discusses how observations support integrated Earth-system science and its applications, and identifies planned enhancements to the contributing observing systems and other requirements for observations and their processing. All types ofmore » observation are considered, but emphasis is placed on those made from space. The origins and development of the integrated view of the Earth system are outlined, noting the interactions between the main components that lead to requirements for integrated science and modelling, and for the observations that guide and support them. What constitutes an Earth-system model is discussed. Summaries are given of key cycles within the Earth system. The nature of Earth observation and the arrangements for international coordination essential for effective operation of global observing systems are introduced. Instances are given of present types of observation, what is already on the roadmap for 2016–2025 and some of the issues to be faced. Observations that are organized on a systematic basis and observations that are made for process understanding and model development, or other research or demonstration purposes, are covered. Specific accounts are given for many of the variables of the Earth system. The current status and prospects for Earth-system modelling are summarized. The evolution towards applying Earth-system models for environmental monitoring and prediction as well as for climate simulation and projection is outlined. General aspects of the improvement of models, whether through refining the

Continued Analysis of EUVE Solar System Observations

NASA Technical Reports Server (NTRS)

Gladstone, G. Randall

2001-01-01

This is the final report for this project. We proposed to continue our work on extracting important results from the EUVE (Extreme UltraViolet Explorer) archive of lunar and jovian system observations. In particular, we planned to: (1) produce several monochromatic images of the Moon at the wavelengths of the brightest solar EUV emission lines; (2) search for evidence of soft X-ray emissions from the Moon and/or X-ray fluorescence at specific EUV wavelengths; (3) search for localized EUV and soft X-ray emissions associated with each of the Galilean satellites; (4) search for correlations between localized Io Plasma Torus (IPT) brightness and volcanic activity on Io; (5) search for soft X-ray emissions from Jupiter; and (6) determine the long term variability of He 58.4 nm emissions from Jupiter, and relate these to solar variability. However, the ADP review panel suggested that the work concentrate on the Jupiter/IPT observations, and provided half the requested funding. Thus we have performed no work on the first two tasks, and instead concentrated on the last three. In addition we used funds from this project to support reduction and analysis of EUVE observations of Venus. While this was not part of the original statement of work, it is entirely in keeping with extracting important results from EUVE solar system observations.

Comparison of Observation Impacts in Two Forecast Systems using Adjoint Methods

NASA Technical Reports Server (NTRS)

Gelaro, Ronald; Langland, Rolf; Todling, Ricardo

2009-01-01

An experiment is being conducted to compare directly the impact of all assimilated observations on short-range forecast errors in different operational forecast systems. We use the adjoint-based method developed by Langland and Baker (2004), which allows these impacts to be efficiently calculated. This presentation describes preliminary results for a "baseline" set of observations, including both satellite radiances and conventional observations, used by the Navy/NOGAPS and NASA/GEOS-5 forecast systems for the month of January 2007. In each system, about 65% of the total reduction in 24-h forecast error is provided by satellite observations, although the impact of rawinsonde, aircraft, land, and ship-based observations remains significant. Only a small majority (50- 55%) of all observations assimilated improves the forecast, while the rest degrade it. It is found that most of the total forecast error reduction comes from observations with moderate-size innovations providing small to moderate impacts, not from outliers with very large positive or negative innovations. In a global context, the relative impacts of the major observation types are fairly similar in each system, although regional differences in observation impact can be significant. Of particular interest is the fact that while satellite radiances have a large positive impact overall, they degrade the forecast in certain locations common to both systems, especially over land and ice surfaces. Ongoing comparisons of this type, with results expected from other operational centers, should lead to more robust conclusions about the impacts of the various components of the observing system as well as about the strengths and weaknesses of the methodologies used to assimilate them.

Observation Scheduling System

NASA Technical Reports Server (NTRS)

Chien, Steve A.; Tran, Daniel Q.; Rabideau, Gregg R.; Schaffer, Steven R.

2011-01-01

Software has been designed to schedule remote sensing with the Earth Observing One spacecraft. The software attempts to satisfy as many observation requests as possible considering each against spacecraft operation constraints such as data volume, thermal, pointing maneuvers, and others. More complex constraints such as temperature are approximated to enable efficient reasoning while keeping the spacecraft within safe limits. Other constraints are checked using an external software library. For example, an attitude control library is used to determine the feasibility of maneuvering between pairs of observations. This innovation can deal with a wide range of spacecraft constraints and solve large scale scheduling problems like hundreds of observations and thousands of combinations of observation sequences.

Efficacy of an ankle brace with a subtalar locking system in inversion control in dynamic movements.

PubMed

Zhang, Songning; Wortley, Michael; Chen, Qingjian; Freedman, Julia

2009-12-01

Controlled laboratory study. To examine effectiveness of an ankle brace with a subtalar locking system in restricting ankle inversion during passive and dynamic movements. Semirigid ankle braces are considered more effective in restricting ankle inversion than other types of brace, but a semirigid brace with a subtalar locking system may be even more effective. Nineteen healthy subjects with no history of major lower extremity injuries were included in the study. Participants performed 5 trials of an ankle inversion drop test and a lateral-cutting movement without wearing a brace and while wearing either the Element (with the subtalar locking system), a Functional ankle brace, or an ASO ankle brace. A 2-way repeated-measures analysis of variance (ANOVA) was used to assess brace differences (P?.05). All 3 braces significantly reduced total passive ankle frontal plane range of motion (ROM), with the Element ankle brace being the most effective. For the inversion drop the results showed significant reductions in peak ankle inversion angle and inversion ROM for all 3 braces compared to the no brace condition; and the peak inversion velocity was also reduced for the Element brace and the Functional brace. In the lateral-cutting movement, a small but significant reduction of the peak inversion angle in early foot contact and the peak eversion velocity at push-off were seen when wearing the Element and the Functional ankle braces compared to the no brace condition. Peak vertical ground reaction force was reduced for the Element brace compared to the ASO brace and the no brace conditions. These results suggest that the tested ankle braces, especially the Element brace, provided effective restriction of ankle inversion during both passive and dynamic movements.

Polymorphic robotic system controlled by an observing camera

NASA Astrophysics Data System (ADS)

Koçer, Bilge; Yüksel, Tugçe; Yümer, M. Ersin; Özen, C. Alper; Yaman, Ulas

2010-02-01

Polymorphic robotic systems, which are composed of many modular robots that act in coordination to achieve a goal defined on the system level, have been drawing attention of industrial and research communities since they bring additional flexibility in many applications. This paper introduces a new polymorphic robotic system, in which the detection and control of the modules are attained by a stationary observing camera. The modules do not have any sensory equipment for positioning or detecting each other. They are self-powered, geared with means of wireless communication and locking mechanisms, and are marked to enable the image processing algorithm detect the position and orientation of each of them in a two dimensional space. Since the system does not depend on the modules for positioning and commanding others, in a circumstance where one or more of the modules malfunction, the system will be able to continue operating with the rest of the modules. Moreover, to enhance the compatibility and robustness of the system under different illumination conditions, stationary reference markers are employed together with global positioning markers, and an adaptive filtering parameter decision methodology is enclosed. To the best of authors' knowledge, this is the first study to introduce a remote camera observer to control modules of a polymorphic robotic system.

Science-Grade Observing Systems as Process Observatories: Mapping and Understanding Nonlinearity and Multiscale Memory with Models and Observations

NASA Astrophysics Data System (ADS)

Barros, A. P.; Wilson, A. M.; Miller, D. K.; Tao, J.; Genereux, D. P.; Prat, O.; Petersen, W. A.; Brunsell, N. A.; Petters, M. D.; Duan, Y.

2015-12-01

Using the planet as a study domain and collecting observations over unprecedented ranges of spatial and temporal scales, NASA's EOS (Earth Observing System) program was an agent of transformational change in Earth Sciences over the last thirty years. The remarkable space-time organization and variability of atmospheric and terrestrial moist processes that emerged from the analysis of comprehensive satellite observations provided much impetus to expand the scope of land-atmosphere interaction studies in Hydrology and Hydrometeorology. Consequently, input and output terms in the mass and energy balance equations evolved from being treated as fluxes that can be used as boundary conditions, or forcing, to being viewed as dynamic processes of a coupled system interacting at multiple scales. Measurements of states or fluxes are most useful if together they map, reveal and/or constrain the underlying physical processes and their interactions. This can only be accomplished through an integrated observing system designed to capture the coupled physics, including nonlinear feedbacks and tipping points. Here, we first review and synthesize lessons learned from hydrometeorology studies in the Southern Appalachians and in the Southern Great Plains using both ground-based and satellite observations, physical models and data-assimilation systems. We will specifically focus on mapping and understanding nonlinearity and multiscale memory of rainfall-runoff processes in mountainous regions. It will be shown that beyond technical rigor, variety, quantity and duration of measurements, the utility of observing systems is determined by their interpretive value in the context of physical models to describe the linkages among different observations. Second, we propose a framework for designing science-grade and science-minded process-oriented integrated observing and modeling platforms for hydrometeorological studies.

Observed ices in the Solar System

USGS Publications Warehouse

Clark, Roger N.; Grundy, Will; Carlson, Robert R.; Noll, Keith; Gudipati, Murthy; Castillo-Rogez, Julie C.

2013-01-01

Ices have been detected and mapped on the Earth and all planets and/or their satellites further from the sun. Water ice is the most common frozen volatile observed and is also unambiguously detected or inferred in every planet and/or their moon(s) except Venus. Carbon dioxide is also extensively found in all systems beyond the Earth except Pluto although it sometimes appears to be trapped rather than as an ice on some objects. The largest deposits of carbon dioxide ice is on Mars. Sulfur dioxide ice is found in the Jupiter system. Nitrogen and methane ices are common beyond the Uranian system. Saturn’s moon Titan probably has the most complex active chemistry involving ices, with benzene (C6H6) and many tentative or inferred compounds including ices of Cyanoacetylene (HC3N), Toluene (C7H8), Cyanogen (C2N2), Acetonitrile (CH3CN), H2O, CO2, and NH3. Confirming compounds on Titan is hampered by its thick smoggy atmosphere. Ammonia was predicted on many icy moons but is notably absent among the definitively detected ices with the possible exception of Enceladus. Comets, storehouses of many compounds that could exist as ices in their nuclei, have only had small amounts of water ice definitively detected on their surfaces. Only one asteroid has had a direct detection of surface water ice, although its presence can be inferred in others. This chapter reviews some of the properties of ices that lead to their detection, and surveys the ices that have been observed on solid surfaces throughout the Solar System.

Synthetic Nucleic Acids and Treatment of Neurological Diseases.

PubMed

Corey, David R

2016-10-01

The ability to control gene expression with antisense oligonucleotides (ASOs) could provide a new treatment strategy for disease. To review the use of ASOs for the treatment of neurological disorders. Articles were identified through a search of PubMed references from 2000 to 2016 for articles describing the use of ASOs to treat disease, with specific attention to neurological disease. We concentrated our review on articles pertaining to activation of frataxin expression (Friedreich's ataxia) and production of active survival motor neuron 2 (SMN2, spinal muscular atrophy). Many neurological diseases are caused by inappropriate expression of a protein. Mutations may reduce expression of a wild-type protein, and strategies to activate expression may provide therapeutic benefit. For other diseases, a mutant protein may be expressed too highly and methods that reduce mutant protein expression might form the basis for drug development. Synthetic ASOs can recognize cellular RNA and control gene expression. Antisense oligonucleotides are not a new concept, but successful clinical development has proceeded at a slow pace. Advances in ASO chemistry, biological understanding, and clinical design are making successful applications more likely. Both laboratory and clinical studies are demonstrating the potential of ASOs as a source of drugs to treat neurological disease.

Anti-sense oligonucleotide therapies for the treatment of hyperlipidaemia.

PubMed

Wierzbicki, Anthony S; Viljoen, Adie

2016-09-01

Anti-sense oligonucleotide (ASO) therapies are a new development in clinical pharmacology offering greater specificity compared to small molecule inhibitors and the ability to target intracellular process' not susceptible to antibody-based therapies. This article reviews the chemical biology of ASOs and related RNA therapeutics. It then reviews the data on their use to treat hyperlipidaemia. Data on mipomersen - an ASO to apolipoprotein B-100(apoB) licensed for treatment of homozygous familial hypercholesterolaemia (FH) is presented. Few effective therapies are available to reduce atehrogenic lipoprotein (a) levels. An ASO therapy to apolipoprotein(a) (ISIS Apo(a)Rx) specifically reduced lipoprotein (a) levels by up to 78%. Treatment options for patients with familial chylomicronaemia syndrome (lipoprotein lipase deficiency; LPLD) or lipodystrophies are highly limited and often inadequate. Volanesorsen, an ASO to apolipoprotein C-3, shows promise in the treatment of LPLD and severe hypertriglyceridaemia as it increases clearance of triglyceride-rich lipoproteins and can normalise triglycerides in these patients. The uptake of the novel ASO therapies is likely to be limited to selected niche groups or orphan diseases. These will include homozygous FH, severe heterozygous FH for mipomersen; LPLD deficiency and lipodystrophy syndromes for volanesorsen and treatment of patients with high elevated Lp(a) levels.

Atmospheric Observations from Unmanned Aircraft Systems

NASA Technical Reports Server (NTRS)

Newman, Paul A.

2008-01-01

Unmanned Aircraft Systems (UASs) provide a new and exciting avenue for atmospheric observations. NASA has a number of UASs. Amongst these are the Ikhana (24 hrs., 7000 km), the Altair (120 hrs., 6500 km), the Aerosonde (30 hrs., 3000 km), and the Global Hawk (30 hrs., 22,000 km). This presentation provides a brief history of UASs which is followed by a description of their capabilities. The presentation concludes by describing an example mission - the UAS Aura Validation Experiment (UAS-AVE). This mission will be flown on the NASA Global Hawk in the Spring/Summer of 2009. The goals fo the mission are to: 1) provide Aura validation observations, 2) sample the break up of the Arctic polar vortex, 3) observed cross-Pacific transport of aerosols and pollutants such as ozone, and 4) sample intense water advective events that impact the U.S. west coast (atmospheric rivers). Because of their range and duration, UASs provide new and exciting opportunities for atmospheric science.

IEOOS: the Spanish Institute of Oceanography Observing System

NASA Astrophysics Data System (ADS)

Tel, E.; Balbin, R.; Cabanas, J. M.; Garcia, M. J.; Garcia-Martinez, M. C.; Gonzalez-Pola, C.; Lavin, A.; Lopez-Jurado, J. L.; Rodriguez, C.; Ruiz-Villarreal, M.; Sanchez-Leal, R. F.; Vargas-Yanez, M.; Velez-Belchi, P.

2015-10-01

Since its foundation, 100 years ago, the Spanish Institute of Oceanography (IEO) has been observing and measuring the ocean characteristics. Here is a summary of the initiatives of the IEO in the field of the operational oceanography (OO). Some systems like the tide gauges network has been working for more than 70 years. The IEO standard sections began at different moments depending on the local projects, and nowadays there are more than 180 coastal stations and deep-sea ones that are systematically sampled, obtaining physical and biochemical measurements. At this moment, the IEO Observing System (IEOOS) includes 6 permanent moorings equipped with currentmeters, an open-sea ocean-meteorological buoy offshore Santander and an SST satellital image reception station. It also supports the Spanish contribution to the ARGO international program with 47 deployed profilers, and continuous monitoring thermosalinometers, meteorological stations and ADCP onboard the IEO research vessels. The system is completed with the IEO contribution to the RAIA and Gibraltar observatories, and the development of regional prediction models. All these systematic measurements allow the IEO to give responses to ocean research activities, official agencies requirements and industrial and main society demands as navigation, resource management, risks management, recreation, etc, as well as for management development pollution-related economic activities or marine ecosystems. All these networks are linked to international initiatives, framed largely in supranational programs Earth observation sponsored by the United Nations or the European Union. The synchronic observation system permits following spatio-temporal description of some events, as new deep water formation in the Mediterranean Sea and the injection of heat to intermediate waters in the Bay of Biscay after some colder northern storms in winter 2005.

Reduced-Rank Array Modes of the California Current Observing System

NASA Astrophysics Data System (ADS)

Moore, Andrew M.; Arango, Hernan G.; Edwards, Christopher A.

2018-01-01

The information content of the ocean observing array spanning the U.S. west coast is explored using the reduced-rank array modes (RAMs) derived from a four-dimensional variational (4D-Var) data assimilation system covering a period of three decades. RAMs are an extension of the original formulation of array modes introduced by Bennett (1985) but in the reduced model state-space explored by the 4D-Var system, and reveal the extent to which this space is activated by the observations. The projection of the RAMs onto the empirical orthogonal functions (EOFs) of the 4D-Var background error correlation matrix provides a quantitative measure of the effectiveness of the measurements in observing the circulation. It is found that much of the space spanned by the background error covariance is unconstrained by the present ocean observing system. The RAM spectrum is also used to introduce a new criterion to prevent 4D-Var from overfitting the model to the observations.

78 FR 14475 - Proposed Establishment of Class E Airspace; Cleveland, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-06

...) Global Positioning System (GPS) Standard Instrument Approach Procedures at Cleveland Regional Jetport. This action would enhance the safety and airspace management of Instrument Flight Rules (IFR...; Airspace Docket No. 12-ASO-47) and be submitted in triplicate to the Docket Management System (see...

The Earth Observing System Terra Mission

NASA Technical Reports Server (NTRS)

Kaufman, Yoram J.; Einaudi, Franco (Technical Monitor)

2000-01-01

Langley's remarkable solar and lunar spectra collected from Mt. Whitney inspired Arrhenius to develop the first quantitative climate model in 1896. After the launch in Dec. 16 1999, NASA's Earth Observing AM Satellite (EOS-Terra) will repeat Langley's experiment, but for the entire planet, thus pioneering a wide array of calibrated spectral observations from space of the Earth System. Conceived in response to real environmental problems, EOS-Terra, in conjunction with other international satellite efforts, will fill a major gap in current efforts by providing quantitative global data sets with a resolution better than 1 km on the physical, chemical and biological elements of the earth system. Thus, like Langley's data, EOS-Terra can revolutionize climate research by inspiring a new generation of climate system models and enable us to assess the human impact on the environment. In the talk I shall review the historical perspective of the Terra mission and the key new elements of the mission. We expect to have first images that demonstrate the most innovative capability from EOS Terra 5 instruments: MODIS - 1.37 micron cirrus cloud channel; 250m daily coverage for clouds and vegetation change; 7 solar channels for land and aerosol studies; new fire channels; Chlorophyll fluorescence; MISR - first 9 multi angle views of clouds and vegetation; MOPITT - first global CO maps and C114 maps; ASTER - Thermal channels for geological studies with 15-90 m resolution.

Microscopic video observation of capillary vessel systems using diffuse back lighting

NASA Astrophysics Data System (ADS)

Sakai, Minako; Arai, Hiroki; Iwai, Toshiaki

2017-04-01

We have been developing a simple and practical video microscopy system based on absorption spectra of biological substance to perform spectroscopic observation of living tissues. The diffuse backlighting effect is actively used in the developed system, which is generated by multiple light scattering in the tissue. It is demonstrated that the light specularly reflected from the skin surface can be completely suppressed in the microscopic observation and the biological activity of the capillary vessel systems distributed under the skin can be successfully observed. As a result, we can confirm the effectiveness of the video microscopy system using diffuse backlighting and the applicability of our developed system.

VRP09 Reduction of Corneal Scarring Following Blast and Burn Injuries to Cornea Using siRNAs Targeting TGFb and CTGF

DTIC Science & Technology

2012-10-01

selective of all gene-targeted, oligonucleotide-based drug approaches (better than ribozymes, antisense oligonucleotides ( ASO ), or microRNAs).(4) We will...respect to a scrambled siRNA control. For the migration assay, a circular region in the middle of the well was removed using a gel removal solution...oligonucleotides, ASOs ) into rabbit corneal cells and found that technique was very effective in delivering ASOs into the stroma and even into the endothelial cell

Accelerating assimilation development for new observing systems using EFSO

NASA Astrophysics Data System (ADS)

Lien, Guo-Yuan; Hotta, Daisuke; Kalnay, Eugenia; Miyoshi, Takemasa; Chen, Tse-Chun

2018-03-01

To successfully assimilate data from a new observing system, it is necessary to develop appropriate data selection strategies, assimilating only the generally useful data. This development work is usually done by trial and error using observing system experiments (OSEs), which are very time and resource consuming. This study proposes a new, efficient methodology to accelerate the development using ensemble forecast sensitivity to observations (EFSO). First, non-cycled assimilation of the new observation data is conducted to compute EFSO diagnostics for each observation within a large sample. Second, the average EFSO conditionally sampled in terms of various factors is computed. Third, potential data selection criteria are designed based on the non-cycled EFSO statistics, and tested in cycled OSEs to verify the actual assimilation impact. The usefulness of this method is demonstrated with the assimilation of satellite precipitation data. It is shown that the EFSO-based method can efficiently suggest data selection criteria that significantly improve the assimilation results.

The Influence of Observation Errors on Analysis Error and Forecast Skill Investigated with an Observing System Simulation Experiment

NASA Technical Reports Server (NTRS)

Prive, N. C.; Errico, R. M.; Tai, K.-S.

2013-01-01

The Global Modeling and Assimilation Office (GMAO) observing system simulation experiment (OSSE) framework is used to explore the response of analysis error and forecast skill to observation quality. In an OSSE, synthetic observations may be created that have much smaller error than real observations, and precisely quantified error may be applied to these synthetic observations. Three experiments are performed in which synthetic observations with magnitudes of applied observation error that vary from zero to twice the estimated realistic error are ingested into the Goddard Earth Observing System Model (GEOS-5) with Gridpoint Statistical Interpolation (GSI) data assimilation for a one-month period representing July. The analysis increment and observation innovation are strongly impacted by observation error, with much larger variances for increased observation error. The analysis quality is degraded by increased observation error, but the change in root-mean-square error of the analysis state is small relative to the total analysis error. Surprisingly, in the 120 hour forecast increased observation error only yields a slight decline in forecast skill in the extratropics, and no discernable degradation of forecast skill in the tropics.

The diversity of planetary system architectures: contrasting theory with observations

NASA Astrophysics Data System (ADS)

Miguel, Y.; Guilera, O. M.; Brunini, A.

2011-10-01

In order to explain the observed diversity of planetary system architectures and relate this primordial diversity to the initial properties of the discs where they were born, we develop a semi-analytical model for computing planetary system formation. The model is based on the core instability model for the gas accretion of the embryos and the oligarchic growth regime for the accretion of the solid cores. Two regimes of planetary migration are also included. With this model, we consider different initial conditions based on recent results of protoplanetary disc observations to generate a variety of planetary systems. These systems are analysed statistically, exploring the importance of several factors that define the planetary system birth environment. We explore the relevance of the mass and size of the disc, metallicity, mass of the central star and time-scale of gaseous disc dissipation in defining the architecture of the planetary system. We also test different values of some key parameters of our model to find out which factors best reproduce the diverse sample of observed planetary systems. We assume different migration rates and initial disc profiles, in the context of a surface density profile motivated by similarity solutions. According to this, and based on recent protoplanetary disc observational data, we predict which systems are the most common in the solar neighbourhood. We intend to unveil whether our Solar system is a rarity or whether more planetary systems like our own are expected to be found in the near future. We also analyse which is the more favourable environment for the formation of habitable planets. Our results show that planetary systems with only terrestrial planets are the most common, being the only planetary systems formed when considering low-metallicity discs, which also represent the best environment for the development of rocky, potentially habitable planets. We also found that planetary systems like our own are not rare in the

The RITMARE Ocean Observing System for the Italian Seas

NASA Astrophysics Data System (ADS)

Crise, A.

2016-02-01

Among its objectives, the Italian RITMARE Flagship Programme has the aim to produce a prototype of the RITMARE Ocean observing system explicitelly designed to provide a powerful infrastructure to the Italian marine science community, to help implement national and Europen environmental regulations and to contribute to the future European Ocean Observing System. The projects takes advantage of the existing platforms (fixed-point moorings, HF and X-band radars, gliders, satellite products), that constitute the basic components of the system. The structure of the RITMARE Ocean observing system is composed by a permanent component (mooring network, satellite images, HF radars) and relocatable component (gliders, drifters, relocatable infrastructures). The increasing number of available relocatable/expandable platforms allow a much larger flexibility in term of allocation of observations but requires an sampling strategy the can be modified according the scientific and socio-economic priorities. As an example, RITMARE focus is set on an experiment on the South Adriatic Pit convective area and its dynamic interactions with the adjacent Bari Canyon cascading site. (Central Mediterranean Sea). Additional effort is paid to support innovation for sensors (e.g. ship-borne LIDAR, stereo-optic directional wave detection, X-band radar innovative products), operational employment of gliders (e.g. Wave Glider) and new class of operational models. The integration can be obtained at different level: the is expected to be achieved at ICT level by defining standard interfaces (NedCDF, SOS) and catalogs in order to facilitate the discovery, viewing and downloading services of data and products. The implementation of a distributed platform-oriented RT repositories adopt a number of THREDDS web servers that act as endpoints for the RITMARE portal. The final aim is to decouple the platforms from the observations, moving from a set of observation to a suite of Essential Ocean Variables by

Study on observation planning of LAMOST focal plane positioning system and its simulation

NASA Astrophysics Data System (ADS)

Zhai, Chao; Jin, Yi; Peng, Xiaobo; Xing, Xiaozheng

2006-06-01

Fiber Positioning System of LAMOST focal plane based on subarea thinking, adopts a parallel controllable positioning plan, the structure is designed as a round area and overlapped each other in order to eliminate the un-observation region. But it also makes the observation efficiency of the system become an important problem. In this paper According to the system, the model of LAMOST focal plane Observation Planning including 4000 fiber positioning units is built, Stars are allocated using netflow algorithm and mechanical collisions are diminished through the retreat algorithm, then the simulation of the system's observation efficiency is carried out. The problem of observation efficiency of LAMOST focal plane is analysed systemic and all-sided from the aspect of overlapped region, fiber positioning units, observation radius, collisions and so on. The observation efficiency of the system in theory is describes and the simulation indicates that the system's observation efficiency is acceptable. The analyses play an indicative role on the design of the LAMOST focal plane structure.

Status of the NASA GMAO Observing System Simulation Experiment

NASA Technical Reports Server (NTRS)

Prive, Nikki C.; Errico, Ronald M.

2014-01-01

An Observing System Simulation Experiment (OSSE) is a pure modeling study used when actual observations are too expensive or difficult to obtain. OSSEs are valuable tools for determining the potential impact of new observing systems on numerical weather forecasts and for evaluation of data assimilation systems (DAS). An OSSE has been developed at the NASA Global Modeling and Assimilation Office (GMAO, Errico et al 2013). The GMAO OSSE uses a 13-month integration of the European Centre for Medium- Range Weather Forecasts 2005 operational model at T511/L91 resolution for the Nature Run (NR). Synthetic observations have been updated so that they are based on real observations during the summer of 2013. The emulated observation types include AMSU-A, MHS, IASI, AIRS, and HIRS4 radiance data, GPS-RO, and conventional types including aircraft, rawinsonde, profiler, surface, and satellite winds. The synthetic satellite wind observations are colocated with the NR cloud fields, and the rawinsondes are advected during ascent using the NR wind fields. Data counts for the synthetic observations are matched as closely as possible to real data counts, as shown in Figure 2. Errors are added to the synthetic observations to emulate representativeness and instrument errors. The synthetic errors are calibrated so that the statistics of observation innovation and analysis increments in the OSSE are similar to the same statistics for assimilation of real observations, in an iterative method described by Errico et al (2013). The standard deviations of observation minus forecast (xo-H(xb)) are compared for the OSSE and real data in Figure 3. The synthetic errors include both random, uncorrelated errors, and an additional correlated error component for some observational types. Vertically correlated errors are included for conventional sounding data and GPS-RO, and channel correlated errors are introduced to AIRS and IASI (Figure 4). HIRS, AMSU-A, and MHS have a component of horizontally

A Digital Video System for Observing and Recording Occultations

NASA Astrophysics Data System (ADS)

Barry, M. A. Tony; Gault, Dave; Pavlov, Hristo; Hanna, William; McEwan, Alistair; Filipović, Miroslav D.

2015-09-01

Stellar occultations by asteroids and outer solar system bodies can offer ground based observers with modest telescopes and camera equipment the opportunity to probe the shape, size, atmosphere, and attendant moons or rings of these distant objects. The essential requirements of the camera and recording equipment are: good quantum efficiency and low noise; minimal dead time between images; good horological faithfulness of the image timestamps; robustness of the recording to unexpected failure; and low cost. We describe an occultation observing and recording system which attempts to fulfil these requirements and compare the system with other reported camera and recorder systems. Five systems have been built, deployed, and tested over the past three years, and we report on three representative occultation observations: one being a 9 ± 1.5 s occultation of the trans-Neptunian object 28978 Ixion (m v =15.2) at 3 seconds per frame; one being a 1.51 ± 0.017 s occultation of Deimos, the 12 km diameter satellite of Mars, at 30 frames per second; and one being a 11.04 ± 0.4 s occultation, recorded at 7.5 frames per second, of the main belt asteroid 361 Havnia, representing a low magnitude drop (Δm v = ~0.4) occultation.

Data acquisition system for operational earth observation missions

NASA Technical Reports Server (NTRS)

Deerwester, J. M.; Alexander, D.; Arno, R. D.; Edsinger, L. E.; Norman, S. M.; Sinclair, K. F.; Tindle, E. L.; Wood, R. D.

1972-01-01

The data acquisition system capabilities expected to be available in the 1980 time period as part of operational Earth observation missions are identified. By data acquisition system is meant the sensor platform (spacecraft or aircraft), the sensors themselves and the communication system. Future capabilities and support requirements are projected for the following sensors: film camera, return beam vidicon, multispectral scanner, infrared scanner, infrared radiometer, microwave scanner, microwave radiometer, coherent side-looking radar, and scatterometer.

The Global Geodetic Observing System: Recent Activities and Accomplishments

NASA Astrophysics Data System (ADS)

Gross, R. S.

2017-12-01

The Global Geodetic Observing System (GGOS) of the International Association of Geodesy (IAG) provides the basis on which future advances in geosciences can be built. By considering the Earth system as a whole (including the geosphere, hydrosphere, cryosphere, atmosphere and biosphere), monitoring Earth system components and their interactions by geodetic techniques and studying them from the geodetic point of view, the geodetic community provides the global geosciences community with a powerful tool consisting mainly of high-quality services, standards and references, and theoretical and observational innovations. The mission of GGOS is: (a) to provide the observations needed to monitor, map and understand changes in the Earth's shape, rotation and mass distribution; (b) to provide the global frame of reference that is the fundamental backbone for measuring and consistently interpreting key global change processes and for many other scientific and societal applications; and (c) to benefit science and society by providing the foundation upon which advances in Earth and planetary system science and applications are built. The goals of GGOS are: (1) to be the primary source for all global geodetic information and expertise serving society and Earth system science; (2) to actively promote, sustain, improve, and evolve the integrated global geodetic infrastructure needed to meet Earth science and societal requirements; (3) to coordinate with the international geodetic services that are the main source of key parameters and products needed to realize a stable global frame of reference and to observe and study changes in the dynamic Earth system; (4) to communicate and advocate the benefits of GGOS to user communities, policy makers, funding organizations, and society. In order to accomplish its mission and goals, GGOS depends on the IAG Services, Commissions, and Inter-Commission Committees. The Services provide the infrastructure and products on which all contributions

IEOOS: the Spanish Institute of Oceanography Observing System

NASA Astrophysics Data System (ADS)

Tel, Elena; Balbin, Rosa; Cabanas, Jose-Manuel; Garcia, Maria-Jesus; Garcia-Martinez, M. Carmen; Gonzalez-Pola, Cesar; Lavin, Alicia; Lopez-Jurado, Jose-Luis; Rodriguez, Carmen; Ruiz-Villarreal, Manuel; Sánchez-Leal, Ricardo F.; Vargas-Yáñez, Manuel; Vélez-Belchí, Pedro

2016-03-01

Since its foundation, 100 years ago, the Spanish Institute of Oceanography (IEO) has been observing and measuring the ocean characteristics. Here is a summary of the initiatives of the IEO in the field of the operational oceanography. Some systems like the tide gauges network has been working for more than 70 years. The standard sections began at different moments depending on the local projects, and nowadays there are more than 180 coastal stations and deep-sea ones that are systematically sampled, obtaining physical and biochemical measurements. At this moment, the Observing System includes six permanent moorings equipped with current meters, an open-sea ocean-meteorological buoy offshore Santander and a sea-surface temperature satellite image station. It also supports the Spanish contribution to the Argo international programme with 47 deployed profilers, and continuous monitoring thermosalinometers, meteorological stations and vessel-mounted acoustic Doppler current profilers on the research vessel fleet. The system is completed with the contribution to the Northwest Iberian peninsula and Gibraltar observatories, and the development of regional prediction models. All these systematic measurements allow the IEO to give responses to ocean research activities, official agencies requirements and industrial and main society demands such as navigation, resource management, risks management, recreation, as well as for management development pollution-related economic activities or marine ecosystems. All these networks are linked to international initiatives, framed largely in supranational programmes of Earth observation sponsored by the United Nations or the European Union. The synchronic observation system permits a spatio-temporal description of some events, such as new deep water formation in the Mediterranean Sea and the injection of heat to intermediate waters in the Bay of Biscay after some colder northern storms in winter 2005.

OSCAR/Surface: Metadata for the WMO Integrated Observing System WIGOS

NASA Astrophysics Data System (ADS)

Klausen, Jörg; Pröscholdt, Timo; Mannes, Jürg; Cappelletti, Lucia; Grüter, Estelle; Calpini, Bertrand; Zhang, Wenjian

2016-04-01

The World Meteorological Organization (WMO) Integrated Global Observing System (WIGOS) is a key WMO priority underpinning all WMO Programs and new initiatives such as the Global Framework for Climate Services (GFCS). It does this by better integrating WMO and co-sponsored observing systems, as well as partner networks. For this, an important aspect is the description of the observational capabilities by way of structured metadata. The 17th Congress of the Word Meteorological Organization (Cg-17) has endorsed the semantic WIGOS metadata standard (WMDS) developed by the Task Team on WIGOS Metadata (TT-WMD). The standard comprises of a set of metadata classes that are considered to be of critical importance for the interpretation of observations and the evolution of observing systems relevant to WIGOS. The WMDS serves all recognized WMO Application Areas, and its use for all internationally exchanged observational data generated by WMO Members is mandatory. The standard will be introduced in three phases between 2016 and 2020. The Observing Systems Capability Analysis and Review (OSCAR) platform operated by MeteoSwiss on behalf of WMO is the official repository of WIGOS metadata and an implementation of the WMDS. OSCAR/Surface deals with all surface-based observations from land, air and oceans, combining metadata managed by a number of complementary, more domain-specific systems (e.g., GAWSIS for the Global Atmosphere Watch, JCOMMOPS for the marine domain, the WMO Radar database). It is a modern, web-based client-server application with extended information search, filtering and mapping capabilities including a fully developed management console to add and edit observational metadata. In addition, a powerful application programming interface (API) is being developed to allow machine-to-machine metadata exchange. The API is based on an ISO/OGC-compliant XML schema for the WMDS using the Observations and Measurements (ISO19156) conceptual model. The purpose of the

Introduction to Observing System Simulation Experiments (OSSEs)

NASA Technical Reports Server (NTRS)

Prive, Nikki C.

2014-01-01

This presentation gives a brief overview of Observing System Simulation Experiments (OSSEs), including what OSSEs are, and how and why they are performed. The intent is to educate the audience in light of the OSSE-related sections of the Forecast Improvement Act (H.R. 2413).

AsyncStageOut: Distributed user data management for CMS Analysis

NASA Astrophysics Data System (ADS)

Riahi, H.; Wildish, T.; Ciangottini, D.; Hernández, J. M.; Andreeva, J.; Balcas, J.; Karavakis, E.; Mascheroni, M.; Tanasijczuk, A. J.; Vaandering, E. W.

2015-12-01

AsyncStageOut (ASO) is a new component of the distributed data analysis system of CMS, CRAB, designed for managing users' data. It addresses a major weakness of the previous model, namely that mass storage of output data was part of the job execution resulting in inefficient use of job slots and an unacceptable failure rate at the end of the jobs. ASO foresees the management of up to 400k files per day of various sizes, spread worldwide across more than 60 sites. It must handle up to 1000 individual users per month, and work with minimal delay. This creates challenging requirements for system scalability, performance and monitoring. ASO uses FTS to schedule and execute the transfers between the storage elements of the source and destination sites. It has evolved from a limited prototype to a highly adaptable service, which manages and monitors the user file placement and bookkeeping. To ensure system scalability and data monitoring, it employs new technologies such as a NoSQL database and re-uses existing components of PhEDEx and the FTS Dashboard. We present the asynchronous stage-out strategy and the architecture of the solution we implemented to deal with those issues and challenges. The deployment model for the high availability and scalability of the service is discussed. The performance of the system during the commissioning and the first phase of production are also shown, along with results from simulations designed to explore the limits of scalability.

Solar System Observations with the James Webb Space Telescope

NASA Technical Reports Server (NTRS)

Norwood, James; Hammel, Heidi; Milam, Stefanie; Stansberry, John; Lunine, Jonathan; Chanover, Nancy; Hines, Dean; Sonneborn, George; Tiscareno, Matthew; Brown, Michael;

Novel classification system of rib fractures observed in infants.

PubMed

Love, Jennifer C; Derrick, Sharon M; Wiersema, Jason M; Pinto, Deborrah C; Greeley, Christopher; Donaruma-Kwoh, Marcella; Bista, Bibek

2013-03-01

Rib fractures are considered highly suspicious for nonaccidental injury in the pediatric clinical literature; however, a rib fracture classification system has not been developed. As an aid and impetus for rib fracture research, we developed a concise schema for classifying rib fracture types and fracture location that is applicable to infants. The system defined four fracture types (sternal end, buckle, transverse, and oblique) and four regions of the rib (posterior, posterolateral, anterolateral, and anterior). It was applied to all rib fractures observed during 85 consecutive infant autopsies. Rib fractures were found in 24 (28%) of the cases. A total of 158 rib fractures were identified. The proposed schema was adequate to classify 153 (97%) of the observed fractures. The results indicate that the classification system is sufficiently robust to classify rib fractures typically observed in infants and should be used by researchers investigating infant rib fractures. © 2013 American Academy of Forensic Sciences.

Linear system identification via backward-time observer models

NASA Technical Reports Server (NTRS)

Juang, Jer-Nan; Phan, Minh

1993-01-01

This paper presents an algorithm to identify a state-space model of a linear system using a backward-time approach. The procedure consists of three basic steps. First, the Markov parameters of a backward-time observer are computed from experimental input-output data. Second, the backward-time observer Markov parameters are decomposed to obtain the backward-time system Markov parameters (backward-time pulse response samples) from which a backward-time state-space model is realized using the Eigensystem Realization Algorithm. Third, the obtained backward-time state space model is converted to the usual forward-time representation. Stochastic properties of this approach will be discussed. Experimental results are given to illustrate when and to what extent this concept works.

Earth observing system - Concepts and implementation strategy

NASA Technical Reports Server (NTRS)

Hartle, R. E.

1986-01-01

The concepts of an Earth Observing System (EOS), an information system being developed by the EOS Science and Mission Requirements Working Group for international use and planned to begin in the 1990s, are discussed. The EOS is designed to study the factors that control the earth's hydrologic cycle, biochemical cycles, and climatologic processes by combining the measurements from remote sensing instruments, in situ measurement devices, and a data and information system. Three EOS platforms are planned to be launched into low, polar, sun-synchronous orbits during the Space Station's Initial Operating Configuration, one to be provided by ESA and two by the United States.

Evaluation of tropical Pacific observing systems using NCEP and GFDL ocean data assimilation systems

NASA Astrophysics Data System (ADS)

Xue, Yan; Wen, Caihong; Yang, Xiaosong; Behringer, David; Kumar, Arun; Vecchi, Gabriel; Rosati, Anthony; Gudgel, Rich

2017-08-01

The TAO/TRITON array is the cornerstone of the tropical Pacific and ENSO observing system. Motivated by the recent rapid decline of the TAO/TRITON array, the potential utility of TAO/TRITON was assessed for ENSO monitoring and prediction. The analysis focused on the period when observations from Argo floats were also available. We coordinated observing system experiments (OSEs) using the global ocean data assimilation system (GODAS) from the National Centers for Environmental Prediction and the ensemble coupled data assimilation (ECDA) from the Geophysical Fluid Dynamics Laboratory for the period 2004-2011. Four OSE simulations were conducted with inclusion of different subsets of in situ profiles: all profiles (XBT, moorings, Argo), all except the moorings, all except the Argo and no profiles. For evaluation of the OSE simulations, we examined the mean bias, standard deviation difference, root-mean-square difference (RMSD) and anomaly correlation against observations and objective analyses. Without assimilation of in situ observations, both GODAS and ECDA had large mean biases and RMSD in all variables. Assimilation of all in situ data significantly reduced mean biases and RMSD in all variables except zonal current at the equator. For GODAS, the mooring data is critical in constraining temperature in the eastern and northwestern tropical Pacific, while for ECDA both the mooring and Argo data is needed in constraining temperature in the western tropical Pacific. The Argo data is critical in constraining temperature in off-equatorial regions for both GODAS and ECDA. For constraining salinity, sea surface height and surface current analysis, the influence of Argo data was more pronounced. In addition, the salinity data from the TRITON buoys played an important role in constraining salinity in the western Pacific. GODAS was more sensitive to withholding Argo data in off-equatorial regions than ECDA because it relied on local observations to correct model biases and

The 2016 Kumamoto-Oita earthquake sequence: aftershock seismicity gap and dynamic triggering in volcanic areas

NASA Astrophysics Data System (ADS)

Uchide, Takahiko; Horikawa, Haruo; Nakai, Misato; Matsushita, Reiken; Shigematsu, Norio; Ando, Ryosuke; Imanishi, Kazutoshi

2016-11-01

The 2016 Kumamoto-Oita earthquake sequence involving three large events ( M w ≥ 6) in the central Kyushu Island, southwest Japan, activated seismicities in two volcanic areas with unusual and puzzling spatial gaps after the largest earthquake ( M w 7.0) of April 16, 2016. We attempt to reveal the seismic process during the sequence by following seismological data analyses. Our hypocenter relocation result implies that the large events ruptured different faults of a complex fault system. A slip inversion analysis of the largest event indicates a large slip in the seismicity gap (Aso gap) in the caldera of Mt. Aso, which probably released accumulated stress and resulted in little aftershock production. We identified that the largest event dynamically triggered a mid-M6 event at Yufuin (80 km northeast of the epicenter), which is consistent with existence of the 20-km long zone where seismicity was activated and surface offset was observed. These findings will help us study the contribution of the identified complexity in fault geometries and the geotherm in the volcanic areas to the revealed seismic process and consequently improve our understanding of the seismo-volcano tectonics.[Figure not available: see fulltext.

Towards An Oceanographic Component Of A Global Earth Observation System Of Systems: Progress And Challenges

NASA Astrophysics Data System (ADS)

Ackleson, S. G.

2012-12-01

Ocean observatories (systems of coordinated sensors and platforms providing real-time in situ observations across multiple temporal and spatial scales) have advanced rapidly during the past several decades with the integration of novel hardware, development of advanced cyber-infrastructures and data management software, and the formation of researcher networks employing fixed, drifting, and mobile assets. These advances have provided persistent, real-time, multi-disciplinary observations representing even the most extreme environmental conditions, enabled unique and informative views of complicated ocean processes, and aided in the development of more accurate and higher fidelity ocean models. Combined with traditional ship-based and remotely sensed observations, ocean observatories have yielded new knowledge across a broad spectrum of earth-ocean scales that would likely not exist otherwise. These developments come at a critical time in human history when the demands of global population growth are creating unprecedented societal challenges associated with rapid climatic change and unsustainable consumption of key ocean resources. Successfully meeting and overcoming these challenges and avoiding the ultimate tragedy of the commons will require greater knowledge of environmental processes than currently exists, including interactions between the ocean, the overlying atmosphere, and the adjacent land and synthesizing new knowledge into effective policy and management structures. To achieve this, researchers must have free and ready access to comprehensive data streams (oceanic, atmospheric, and terrestrial), regardless of location and collection system. While the precedent for the concept of free and open access to environmental data is not new (it traces back to the International Geophysical Year, 1957), implementing procedures and standards on a global scale is proving to be difficult, both logistically and politically. Observatories have been implemented in many

Observer Use of Standardized Observation Protocols in Consequential Observation Systems

ERIC Educational Resources Information Center

Bell, Courtney A.; Yi, Qi; Jones, Nathan D.; Lewis, Jennifer M.; McLeod, Monica; Liu, Shuangshuang

2014-01-01

Evidence from a handful of large-scale studies suggests that although observers can be trained to score reliably using observation protocols, there are concerns related to initial training and calibration activities designed to keep observers scoring accurately over time (e.g., Bell, et al, 2012; BMGF, 2012). Studies offer little insight into how…

32 CFR 1656.1 - Purpose; definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Service Office (ASO). An office to administer the Alternative Service Program in a specified geographical area. (3) Alternative Service Office Manager (ASOM). The head of the ASO. (4) Alternative Service Work...

32 CFR 1656.1 - Purpose; definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Service Office (ASO). An office to administer the Alternative Service Program in a specified geographical area. (3) Alternative Service Office Manager (ASOM). The head of the ASO. (4) Alternative Service Work...

32 CFR 1656.1 - Purpose; definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Service Office (ASO). An office to administer the Alternative Service Program in a specified geographical area. (3) Alternative Service Office Manager (ASOM). The head of the ASO. (4) Alternative Service Work...

32 CFR 1656.1 - Purpose; definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Service Office (ASO). An office to administer the Alternative Service Program in a specified geographical area. (3) Alternative Service Office Manager (ASOM). The head of the ASO. (4) Alternative Service Work...

32 CFR 1656.1 - Purpose; definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Service Office (ASO). An office to administer the Alternative Service Program in a specified geographical area. (3) Alternative Service Office Manager (ASOM). The head of the ASO. (4) Alternative Service Work...

NASA's Earth Observing System (EOS): Observing the Atmosphere, Land, Oceans, and Ice from Space

NASA Technical Reports Server (NTRS)

King, Michael D.

2004-01-01

The Earth Observing System (EOS) is a space-based observing system comprised of a series of satellite sensors by which scientists can monitor the Earth, a Data and Information System (EOSDIS) enabling researchers worldwide to access the satellite data, and an interdisciplinary science research program to interpret the satellite data. During this year, the last of the first series of EOS missions, Aura, was launched. Aura is designed exclusively to conduct research on the composition, chemistry, and dynamics of the Earth's upper and lower atmosphere, employing multiple instruments on a single spacecraft. Aura is the third in a series of major Earth observing satellites to study the environment and climate change and is part of NASA's Earth Science Enterprise. The first and second missions, Terra and Aqua, are designed to study the land, oceans, atmospheric constituents (aerosols, clouds, temperature, and water vapor), and the Earth's radiation budget. The other seven EOS spacecraft include satellites to study (i) land cover & land use change, (ii) solar irradiance and solar spectral variation, (iii) ice volume, (iv) ocean processes (vector wind and sea surface topography), and (v) vertical variations of clouds, water vapor, and aerosols up to and including the stratosphere. Aura's chemistry measurements will also follow up on measurements that began with NASA's Upper Atmosphere Research Satellite and continue the record of satellite ozone data collected from the TOMS missions. In this presentation I will describe how scientists are using EOS data to examine the health of the earth's atmosphere, including atmospheric chemistry, aerosol properties, and cloud properties, with a special but not exclusive look at the latest earth observing mission, Aura.

NASA's Earth Observing System (EOS): Observing the Atmosphere, Land, Oceans, and Ice from Space

NASA Technical Reports Server (NTRS)

King, Michael D.

2005-01-01

The Earth Observing System (EOS) is a space-based observing system comprised of a series of satellite sensors by whch scientists can monitor the Earth, a Data and Information System (EOSDIS) enabling researchers worldwide to access the satellite data, and an interdisciplinary science research program to interpret the satellite data. During this year, the last of the first series of EOS missions, Aura, was launched. Aura is designed exclusively to conduct research on the composition, chemistry, and dynamics of the Earth's upper and lower atmosphere, employing multiple instruments on a single spacecraft. Aura is the third in a series of major Earth observing satellites to study the environment and climate change and is part of NASA's Earth Science Enterprise. The first and second missions, Terra and Aqua, are designed to study the land, oceans, atmospheric constituents (aerosols, clouds, temperature, and water vapor), and the Earth's radiation budget. The other seven EOS spacecraft include satellites to study (i) land cover & land use change, (ii) solar irradiance and solar spectral variation, (iii) ice volume, (iv) ocean processes (vector wind and sea surface topography), and (v) vertical variations of clouds, water vapor, and aerosols up to and including the stratosphere. Aura's chemistry measurements will also follow up on measurements that began with NASA's Upper Atmosphere Research Satellite and continue the record of satellite ozone data collected from the TOMS missions. In this presentation I will describe how scientists are using EOS data to examine the health of the earth's atmosphere, including atmospheric chemistry, aerosol properties, and cloud properties, with a special look at the latest earth observing mission, Aura.

78 FR 14473 - Proposed Establishment of Class E Airspace; Sanibel, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-06

...) Global Positioning System (GPS) special Standard Instrument Approach Procedure (SIAP) serving Sanibel Island Heliport. This action would enhance the safety and airspace management of Instrument Flight Rules...; Airspace Docket No. 12-ASO-18) and be submitted in triplicate to the Docket Management System (see...

77 FR 39652 - Proposed Establishment of Class E Airspace; La Belle, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

...) Global Positioning System (GPS) Standard Instrument Approach Procedures at La Belle Municipal Airport. This action would enhance the safety and airspace management of Instrument Flight Rules (IFR...; Airspace Docket No. 12-ASO-29) and be submitted in triplicate to the Docket Management System (see...

76 FR 49390 - Proposed Establishment of Class E Airspace; Palmyra, PA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-10

...) Global Positioning System (GPS) Standard Instrument Approach Procedures at Reigle Field. This action would enhance the safety and airspace management of Instrument Flight Rules (IFR) operations at the...; Airspace Docket No. 11-ASO-17) and be submitted in triplicate to the Docket Management System (see...

78 FR 52111 - Proposed Establishment of Class E Airspace; Aliceville, AL

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-22

... (RNAV) Global Positioning System (GPS) Standard Instrument Approach Procedure (SIAP) serving George Downer Airport. This action would enhance the safety and airspace management of Instrument Flight Rules...; Airspace Docket No. 13-ASO-7) and be submitted in triplicate to the Docket Management System (see ADDRESSES...

Coupling fractionation and batch desorption to understand arsenic and fluoride co-contamination in the aquifer system.

PubMed

Kumar, Manish; Das, Nilotpal; Goswami, Ritusmita; Sarma, Kali Prasad; Bhattacharya, Prosun; Ramanathan, A L

2016-12-01

The present work is an attempt to study As and F+ coevality using laboratory based assays which couples fractionation and batch dissolution experiments. Sequential extraction procedure (SEP) resulting into five "operationally defined phases", was performed on sediment and soil samples collected from the Brahmaputra flood plains, Assam, India. High correlation between the Fe (hydr)oxide fraction and total As content of the soil/sediment sample indicates the involvement of Fe (hydr)oxides as the principal source of As. F - being an anion has high potential to be sorbed onto positively charged surfaces. Findings of the SEP were used to design the batch desorption experiments by controlling the Fe (hydr)oxide content of the soil/sediment. Desorption of As and F - was observed under acidic, neutral and alkaline pH from untreated and Fe (hydr)oxide removed samples. Highest amount of As and F - were found to be released from untreated samples under alkaline pH, while the amount leached from samples with no Fe (hydr)oxide was low. The study showed that the Fe (hydr)oxide fraction commonly found in the soils and sediments, had high affinity for negatively charged species like F - oxyanions of As, AsO 4 3- (arsenate) and AsO 3 3- (arsenite). Fe (hydr)oxide fraction was found to play the major role in co-evolution of As and F - . Two sorption coefficients were proposed based on easily leachable fraction and As present in the groundwater of sampling location for understanding of contamination vulnerability from the leaching. Copyright © 2016 Elsevier Ltd. All rights reserved.

Abuse Experiences of Family Members, Child Maltreatment, and the Development of Sex Offending Among Incarcerated Adolescent Males.

PubMed

McCuish, Evan C; Cale, Jesse; Corrado, Raymond R

2017-02-01

Child sexual abuse is considered a risk factor for the development of sexual offending in adolescence. Beyond this, comparisons of the risk factor profiles between adolescent sex offenders (ASOs) and adolescent non-sex offenders (ANSOs) have uncovered minimal differences. However, differences between ASOs and ANSOs in terms of patterns in the abuse histories of their family members have rarely been studied. The aim in the current study was to retrospectively examine histories of abuse among family members of ASOs compared with ANSOs to determine whether and how these were related to youth abuse experiences and sexual offending in adolescence. The current study is based on a sample of 482 incarcerated male adolescents (ASOs = 67, ANSOs = 415). Latent class analysis was conducted to determine multidimensional familial abuse profiles, and a series of logistic regression models were used to examine the relationship between family abuse profiles, youth abuse experiences, and adolescent sexual offending. Overall, familial abuse profiles were related to subsequent youth abuse experiences and sexual offending, and these abuse profiles differentiated ASOs and ANSOs.

The 1990 Reference Handbook: Earth Observing System

NASA Technical Reports Server (NTRS)

1990-01-01

An overview of the Earth Observing System (EOS) including goals and requirements is given. Its role in the U.S. Global Change Research Program and the International--Biosphere Program is addressed. The EOS mission requirements, science, fellowship program, data and information systems architecture, data policy, space measurement, and mission elements are presented along with the management of EOS. Descriptions of the facility instruments, instrument investigations, and interdisciplinary investigations are also present. The role of the National Oceanic and Atmospheric Administration in the mission is mentioned.

ASO: Antistreptolysin O titer

MedlinePlus

... of Group A Streptococcal Infections. American Society for Microbiology [On-line journal]. Clin Microbiol Rev . 2000 July; ... Forbes, B. et. al. (© 2007). Bailey & Scott's Diagnostic Microbiology, 12th Edition: Mosby Elsevier Press, St. Louis, MO. ...

Development of KIAPS Observation Processing Package for Data Assimilation System

NASA Astrophysics Data System (ADS)

Kang, Jeon-Ho; Chun, Hyoung-Wook; Lee, Sihye; Han, Hyun-Jun; Ha, Su-Jin

2015-04-01

The Korea Institute of Atmospheric Prediction Systems (KIAPS) was founded in 2011 by the Korea Meteorological Administration (KMA) to develop Korea's own global Numerical Weather Prediction (NWP) system as nine year (2011-2019) project. Data assimilation team at KIAPS has been developing the observation processing system (KIAPS Package for Observation Processing: KPOP) to provide optimal observations to the data assimilation system for the KIAPS Global Model (KIAPS Integrated Model - Spectral Element method based on HOMME: KIM-SH). Currently, the KPOP is capable of processing the satellite radiance data (AMSU-A, IASI), GPS Radio Occultation (GPS-RO), AIRCRAFT (AMDAR, AIREP, and etc…), and synoptic observation (SONDE and SURFACE). KPOP adopted Radiative Transfer for TOVS version 10 (RTTOV_v10) to get brightness temperature (TB) for each channel at top of the atmosphere (TOA), and Radio Occultation Processing Package (ROPP) 1-dimensional forward module to get bending angle (BA) at each tangent point. The observation data are obtained from the KMA which has been composited with BUFR format to be converted with ODB that are used for operational data assimilation and monitoring at the KMA. The Unified Model (UM), Community Atmosphere - Spectral Element (CAM-SE) and KIM-SH model outputs are used for the bias correction (BC) and quality control (QC) of the observations, respectively. KPOP provides radiance and RO data for Local Ensemble Transform Kalman Filter (LETKF) and also provides SONDE, SURFACE and AIRCRAFT data for Three-Dimensional Variational Assimilation (3DVAR). We are expecting all of the observation type which processed in KPOP could be combined with both of the data assimilation method as soon as possible. The preliminary results from each observation type will be introduced with the current development status of the KPOP.

Overcoming imatinib resistance conferred by the BIM deletion polymorphism in chronic myeloid leukemia with splice-switching antisense oligonucleotides

PubMed Central

Liu, Jun; Bhadra, Malini; Sinnakannu, Joanna Rajeswary; Yue, Wan Lin; Tan, Cheryl Weiqi; Rigo, Frank; Ong, S.Tiong; Roca, Xavier

2017-01-01

Many tyrosine kinase-driven cancers, including chronic myeloid leukemia (CML), are characterized by high response rates to specific tyrosine kinase inhibitors (TKIs) like imatinib. In East Asians, primary imatinib resistance is caused by a deletion polymorphism in Intron 2 of the BIM gene, whose product is required for TKI-induced apoptosis. The deletion biases BIM splicing from exon 4 to exon 3, generating splice isoforms lacking the exon 4-encoded pro-apoptotic BH3 domain, which impairs the ability of TKIs to induce apoptosis. We sought to identify splice-switching antisense oligonucleotides (ASOs) that block exon 3 but enhance exon 4 splicing, and thereby resensitize BIM deletion-containing cancers to imatinib. First, we mapped multiple cis-acting splicing elements around BIM exon 3 by minigene mutations, and found an exonic splicing enhancer acting via SRSF1. Second, by a systematic ASO walk, we isolated ASOs that corrected the aberrant BIM splicing. Eight of 67 ASOs increased exon 4 levels in BIM deletion-containing cells, and restored imatinib-induced apoptosis and TKI sensitivity. This proof-of-principle study proves that resistant CML cells by BIM deletion polymorphism can be resensitized to imatinib via splice-switching BIM ASOs. Future optimizations might yield a therapeutic ASO as precision-medicine adjuvant treatment for BIM-polymorphism-associated TKI-resistant CML and other cancers. PMID:29100409

Overcoming imatinib resistance conferred by the BIM deletion polymorphism in chronic myeloid leukemia with splice-switching antisense oligonucleotides.

PubMed

Liu, Jun; Bhadra, Malini; Sinnakannu, Joanna Rajeswary; Yue, Wan Lin; Tan, Cheryl Weiqi; Rigo, Frank; Ong, S Tiong; Roca, Xavier

2017-09-29

Many tyrosine kinase-driven cancers, including chronic myeloid leukemia (CML), are characterized by high response rates to specific tyrosine kinase inhibitors (TKIs) like imatinib. In East Asians, primary imatinib resistance is caused by a deletion polymorphism in Intron 2 of the BIM gene, whose product is required for TKI-induced apoptosis. The deletion biases BIM splicing from exon 4 to exon 3, generating splice isoforms lacking the exon 4-encoded pro-apoptotic BH3 domain, which impairs the ability of TKIs to induce apoptosis. We sought to identify splice-switching antisense oligonucleotides (ASOs) that block exon 3 but enhance exon 4 splicing, and thereby resensitize BIM deletion-containing cancers to imatinib. First, we mapped multiple cis -acting splicing elements around BIM exon 3 by minigene mutations, and found an exonic splicing enhancer acting via SRSF1. Second, by a systematic ASO walk, we isolated ASOs that corrected the aberrant BIM splicing. Eight of 67 ASOs increased exon 4 levels in BIM deletion-containing cells, and restored imatinib-induced apoptosis and TKI sensitivity. This proof-of-principle study proves that resistant CML cells by BIM deletion polymorphism can be resensitized to imatinib via splice-switching BIM ASOs. Future optimizations might yield a therapeutic ASO as precision-medicine adjuvant treatment for BIM -polymorphism-associated TKI-resistant CML and other cancers.

Antisense oligonucleotide reduction of apoB-ameliorated atherosclerosis in LDL receptor-deficient mice[S

PubMed Central

Mullick, Adam E.; Fu, Wuxia; Graham, Mark J.; Lee, Richard G.; Witchell, Donna; Bell, Thomas A.; Whipple, Charles P.; Crooke, Rosanne M.

2011-01-01

Chronic elevations of plasma apolipoprotein B (apoB) are strongly associated with cardiovascular disease. We have previously demonstrated that inhibition of hepatic apoB mRNA using antisense oligonucleotides (ASO) results in reductions of apoB, VLDL, and LDL in several preclinical animal models and humans. In this study, we evaluated the anti-atherogenic effects of a murine-specific apoB ASO (ISIS 147764) in hypercholesterolemic LDLr deficient (LDLr−/−) mice. ISIS 147764 was administered weekly at 25-100 mg/kg for 10-12 weeks and produced dose-dependent reductions of hepatic apoB mRNA and plasma LDL by 60-90%. No effects on these parameters were seen in mice receiving control ASOs. ApoB ASO treatment also produced dose-dependent reductions of aortic en face and sinus atherosclerosis from 50-90%, with high-dose treatment displaying less disease than the saline-treated, chow-fed LDLr−/− mice. No changes in intestinal cholesterol absorption were seen with apoB ASO treatment, suggesting that the cholesterol-lowering pharmacology of 147764 was primarily due to inhibition of hepatic apoB synthesis and secretion. In summary, ASO-mediated suppression of apoB mRNA expression profoundly reduced plasma lipids and atherogenesis in LDLr−/− mice, leading to the hypothesis that apoB inhibition in humans with impaired LDLr activity may produce similar effects. PMID:21343632

Antisense oligonucleotides targeting translation inhibitory elements in 5' UTRs can selectively increase protein levels.

PubMed

Liang, Xue-Hai; Sun, Hong; Shen, Wen; Wang, Shiyu; Yao, Joyee; Migawa, Michael T; Bui, Huynh-Hoa; Damle, Sagar S; Riney, Stan; Graham, Mark J; Crooke, Rosanne M; Crooke, Stanley T

2017-09-19

A variety of diseases are caused by deficiencies in amounts or activity of key proteins. An approach that increases the amount of a specific protein might be of therapeutic benefit. We reasoned that translation could be specifically enhanced using trans-acting agents that counter the function of negative regulatory elements present in the 5' UTRs of some mRNAs. We recently showed that translation can be enhanced by antisense oligonucleotides (ASOs) that target upstream open reading frames. Here we report the amount of a protein can also be selectively increased using ASOs designed to hybridize to other translation inhibitory elements in 5' UTRs. Levels of human RNASEH1, LDLR, and ACP1 and of mouse ACP1 and ARF1 were increased up to 2.7-fold in different cell types and species upon treatment with chemically modified ASOs targeting 5' UTR inhibitory regions in the mRNAs encoding these proteins. The activities of ASOs in enhancing translation were sequence and position dependent and required helicase activity. The ASOs appear to improve the recruitment of translation initiation factors to the target mRNA. Importantly, ASOs targeting ACP1 mRNA significantly increased the level of ACP1 protein in mice, suggesting that this approach has therapeutic and research potentials. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Experimental land observing data system feasibility study

NASA Technical Reports Server (NTRS)

Buckley, J. L.; Kraiman, H.

1982-01-01

An end-to-end data system to support a Shuttle-based Multispectral Linear Array (MLA) mission in the mid-1980's was defined. The experimental Land Observing System (ELOS) is discussed. A ground system that exploits extensive assets from the LANDSAT-D Program to effectively meet the objectives of the ELOS Mission was defined. The goal of 10 meter pixel precision, the variety of data acquisition capabilities, and the use of Shuttle are key to the mission requirements, Ground mission management functions are met through the use of GSFC's Multi-Satellite Operations Control Center (MSOCC). The MLA Image Generation Facility (MIGF) combines major hardware elements from the Applications Development Data System (ADDS) facility and LANDSAT Assessment System (LAS) with a special purpose MLA interface unit. LANDSAT-D image processing techniques, adapted to MLA characteristics, form the basis for the use of existing software and the definition of new software required.

In vitro and in vivo inhibition of proangiogenic retinal phenotype by an antisense oligonucleotide downregulating uPAR expression.

PubMed

Lulli, Matteo; Cammalleri, Maurizio; Granucci, Irene; Witort, Ewa; Bono, Silvia; Di Gesualdo, Federico; Lupia, Antonella; Loffredo, Rosa; Casini, Giovanni; Dal Monte, Massimo; Capaccioli, Sergio

2017-08-26

Neoangiogenesis is the main pathogenic event involved in a variety of retinal diseases. It has been recently demonstrated that inhibiting the urokinase-type plasminogen activator receptor (uPAR) results in reduced angiogenesis in a mouse model of oxygen-induced retinopathy (OIR), establishing uPAR as a therapeutic target in proliferative retinopathies. Here, we evaluated in cultured human retinal endothelial cells (HRECs) and in OIR mice the potential of a specific antisense oligodeoxyribonucleotide (ASO) in blocking the synthesis of uPAR and in providing antiangiogenic effects. uPAR expression in HRECs was inhibited by lipofection with the phosphorotioated 5'-CGGCGGGTGACCCATGTG-3' ASO-uPAR, complementary to the initial translation site of uPAR mRNA. Inhibition of uPAR expression via ASO-uPAR was evaluated in HRECs by analyzing VEGF-induced tube formation and migration. In addition, the well-established and reproducible murine OIR model was used to induce retinal neovascularization in vivo. OIR mice were injected intraperitoneally with ASO-uPAR and retinopathy was evaluated considering the extent of the avascular area in the central retina and neovascular tuft formation. The ASO-uPAR specifically decreased uPAR mRNA and protein levels in HRECs and mitigated VEGF-induced tube formation and cell migration. Noteworthy, in OIR mice ASO-uPAR administration reduced both the avascular area and the formation of neovascular tufts. In conclusion, although the extrapolation of these experimental findings to the clinic is not straightforward, ASO-uPAR may be considered a potential therapeutic tool for treatment of proliferative retinal diseases. Copyright © 2017 Elsevier Inc. All rights reserved.

Factor XI Antisense Oligonucleotide for Prevention of Venous Thrombosis

PubMed Central

Büller, Harry R.; Bethune, Claudette; Bhanot, Sanjay; Gailani, David; Monia, Brett P.; Raskob, Gary E.; Segers, Annelise; Verhamme, Peter; Weitz, Jeffrey I.

2015-01-01

BACKGROUND Experimental data indicate that reducing factor XI levels attenuates thrombosis without causing bleeding, but the role of factor XI in the prevention of postoperative venous thrombosis in humans is unknown. FXI-ASO (ISIS 416858) is a second-generation antisense oligonucleotide that specifically reduces factor XI levels. We compared the efficacy and safety of FXI-ASO with those of enoxaparin in patients undergoing total knee arthroplasty. METHODS In this open-label, parallel-group study, we randomly assigned 300 patients who were undergoing elective primary unilateral total knee arthroplasty to receive one of two doses of FXI-ASO (200 mg or 300 mg) or 40 mg of enoxaparin once daily. The primary efficacy outcome was the incidence of venous thromboembolism (assessed by mandatory bilateral venography or report of symptomatic events). The principal safety outcome was major or clinically relevant nonmajor bleeding. RESULTS Around the time of surgery, the mean (±SE) factor XI levels were 0.38±0.01 units per milliliter in the 200-mg FXI-ASO group, 0.20±0.01 units per milliliter in the 300-mg FXI-ASO group, and 0.93±0.02 units per milliliter in the enoxaparin group. The primary efficacy outcome occurred in 36 of 134 patients (27%) who received the 200-mg dose of FXI-ASO and in 3 of 71 patients (4%) who received the 300-mg dose of FXI-ASO, as compared with 21 of 69 patients (30%) who received enoxaparin. The 200-mg regimen was noninferior, and the 300-mg regimen was superior, to enoxaparin (P<0.001). Bleeding occurred in 3%, 3%, and 8% of the patients in the three study groups, respectively. CONCLUSIONS This study showed that factor XI contributes to postoperative venous thromboembolism; reducing factor XI levels in patients undergoing elective primary unilateral total knee arthroplasty was an effective method for its prevention and appeared to be safe with respect to the risk of bleeding. (Funded by Isis Pharmaceuticals; FXI-ASO TKA ClinicalTrials.gov number

Antisense oligonucleotides for the treatment of dyslipidaemia.

PubMed

Visser, Maartje E; Witztum, Joseph L; Stroes, Erik S G; Kastelein, John J P

2012-06-01

Antisense oligonucleotides (ASOs) are short synthetic analogues of natural nucleic acids designed to specifically bind to a target messenger RNA (mRNA) by Watson-Crick hybridization, inducing selective degradation of the mRNA or prohibiting translation of the selected mRNA into protein. Antisense technology has the ability to inhibit unique targets with high specificity and can be used to inhibit synthesis of a wide range of proteins that could influence lipoprotein levels and other targets. A number of different classes of antisense agents are under development. To date, mipomersen, a 2'-O-methoxyethyl phosphorothioate 20-mer ASO, is the most advanced ASO in clinical development. It is a second-generation ASO developed to inhibit the synthesis of apolipoprotein B (apoB)-100 in the liver. In Phase 3 clinical trials, mipomersen has been shown to significantly reduce plasma low-density lipoprotein cholesterol (LDL-c) as well as other atherogenic apoB containing lipoproteins such as lipoprotein (a) [Lp(a)] and small-dense LDL particles. Although concerns have been raised because of an increase in intrahepatic triglyceride content, preliminary data from long-term studies suggest that with continued treatment, liver fat levels tend to stabilize or decline. Further studies are needed to evaluate potential clinical relevance of these changes. Proprotein convertase subtilisin/kexin-9 (PCSK9) is another promising novel target for lowering LDL-c by ASOs. Both second-generation ASOs and ASOs using locked nucleic acid technology have been developed to inhibit PCSK9 and are under clinical development. Other targets currently being addressed include apoC-III and apo(a) or Lp(a). By directly inhibiting the synthesis of specific proteins, ASO technology offers a promising new approach to influence the metabolism of lipids and to control lipoprotein levels. Its application to a wide variety of potential targets can be expected if these agents prove to be clinically safe and

An optimization approach for observation association with systemic uncertainty applied to electro-optical systems

NASA Astrophysics Data System (ADS)

Worthy, Johnny L.; Holzinger, Marcus J.; Scheeres, Daniel J.

2018-06-01

The observation to observation measurement association problem for dynamical systems can be addressed by determining if the uncertain admissible regions produced from each observation have one or more points of intersection in state space. An observation association method is developed which uses an optimization based approach to identify local Mahalanobis distance minima in state space between two uncertain admissible regions. A binary hypothesis test with a selected false alarm rate is used to assess the probability that an intersection exists at the point(s) of minimum distance. The systemic uncertainties, such as measurement uncertainties, timing errors, and other parameter errors, define a distribution about a state estimate located at the local Mahalanobis distance minima. If local minima do not exist, then the observations are not associated. The proposed method utilizes an optimization approach defined on a reduced dimension state space to reduce the computational load of the algorithm. The efficacy and efficiency of the proposed method is demonstrated on observation data collected from the Georgia Tech Space Object Research Telescope.

The function of the earth observing system - Data information system Distributed Active Archive Centers

NASA Technical Reports Server (NTRS)

Lapenta, C. C.

1992-01-01

The functionality of the Distributed Active Archive Centers (DAACs) which are significant elements of the Earth Observing System Data and Information System (EOSDIS) is discussed. Each DAAC encompasses the information management system, the data archival and distribution system, and the product generation system. The EOSDIS DAACs are expected to improve the access to earth science data set needed for global change research.

77 FR 23597 - Revocation of Class E Airspace; Southport, NC, and Establishment of Class E Airspace; Oak Island, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-20

... designated as South Brunswick County Airport, Southport, NC, (old name), and establish Class E airspace... surface of the earth. * * * * * ASO NC E5 Southport, NC [Removed] * * * * * ASO NC E5 Oak Island, NC [New...

Chandra X-Ray Observatory Observations of the Jovian System

NASA Technical Reports Server (NTRS)

Elsner, R. F.; Bhardwaj, A.; Gladstone, R.; Waite, J. H.; Ford, P.; Branduari-Raymont, G.

2005-01-01

Chandra X-ray Observatory (CXO) and XMM-Newton observations of x-rays from the Jovian system have answered questions that arose from early observations with the Einstein and Rosat X-ray Observatories, but in the process of vastly increasing our knowledge of x-ray emission from Jupiter and its environs they have also raised new questions and point to new opportunities for future studies. We will review recent x-ray results on the Jovian system, from the point of view of the CXO, and discuss various questions that have arisen in the course of our studies. We will discuss prospects for more observations in the immediate future, and how they might address open questions. Finally we will briefly describe ways in which an imaging x-ray spectrometer in the vicinity of the Jovian system could provide a wealth of data and results concerning Jupiter's x-ray auroral and disk emission, elemental abundance measurements for the Galilean moons, and detailed studies of x-ray emission from the Io Plasma Torus.

Earth observing system. Data and information system. Volume 2A: Report of the EOS Data Panel

NASA Technical Reports Server (NTRS)

1986-01-01

The purpose of this report is to provide NASA with a rationale and recommendations for planning, implementing, and operating an Earth Observing System data and information system that can evolve to meet the Earth Observing System's needs in the 1990s. The Earth Observing System (Eos), defined by the Eos Science and Mission Requirements Working Group, consists of a suite of instruments in low Earth orbit acquiring measurements of the Earth's atmosphere, surface, and interior; an information system to support scientific research; and a vigorous program of scientific research, stressing study of global-scale processes that shape and influence the Earth as a system. The Eos data and information system is conceived as a complete research information system that would transcend the traditional mission data system, and include additional capabilties such as maintaining long-term, time-series data bases and providing access by Eos researchers to relevant non-Eos data. The Working Group recommends that the Eos data and information system be initiated now, with existing data, and that the system evolve into one that can meet the intensive research and data needs that will exist when Eos spacecraft are returning data in the 1990s.

77 FR 770 - Proposed Revocation of Class E Airspace; Southport, NC, and Proposed Establishment of Class E...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-06

... County Airport, Southport, NC, (old name), and establish Class E airspace extending upward from 700 feet... surface of the earth. * * * * * ASO NC E5 Southport, NC [Removed] * * * * * ASO NC E5 Oak Island, NC [New...

Industrial use of land observation satellite systems

NASA Technical Reports Server (NTRS)

Henderson, F. B., III

1984-01-01

The principal industrial users of land observation satellite systems are the geological industries; oil/gas, mining, and engineering/environmental companies. The primary system used is LANDSAT/MSS. Currently, use is also being made of the limited amounts of SKYLAB photography, SEASAT and SIR-A radar, and the new LANDSAT/TM data available. Although considered experimental, LANDSAT data is now used operationally by several hundred exploration and engineering companies worldwide as a vastly improved geological mapping tool to help direct more expensive geophysical and drilling phases, leading to more efficient decision-making and results. Future needs include global LANDSAT/TM; higher spatial resolution; stereo and radar; improved data handling, processing distribution and archiving systems, and integrated geographical information systems (GIS). For a promising future, governments must provide overall continuity (government and/or private sector) of such systems, insure continued government R and D, and commit to operating internationally under the civil Open Skies policy.

Spatial and temporal seismic velocity changes on Kyushu Island during the 2016 Kumamoto earthquake

PubMed Central

Nimiya, Hiro; Ikeda, Tatsunori; Tsuji, Takeshi

2017-01-01

Monitoring of earthquake faults and volcanoes contributes to our understanding of their dynamic mechanisms and to our ability to predict future earthquakes and volcanic activity. We report here on spatial and temporal variations of seismic velocity around the seismogenic fault of the 2016 Kumamoto earthquake [moment magnitude (Mw) 7.0] based on ambient seismic noise. Seismic velocity near the rupture faults and Aso volcano decreased during the earthquake. The velocity reduction near the faults may have been due to formation damage, a change in stress state, and an increase in pore pressure. Further, we mapped the post-earthquake fault-healing process. The largest seismic velocity reduction observed at Aso volcano during the earthquake was likely caused by pressurized volcanic fluids, and the large increase in seismic velocity at the volcano’s magma body observed ~3 months after the earthquake may have been a response to depressurization caused by the eruption. This study demonstrates the usefulness of continuous monitoring of faults and volcanoes. PMID:29202026

Virtual Estimator for Piecewise Linear Systems Based on Observability Analysis

PubMed Central

Morales-Morales, Cornelio; Adam-Medina, Manuel; Cervantes, Ilse; Vela-Valdés and, Luis G.; García Beltrán, Carlos Daniel

2013-01-01

This article proposes a virtual sensor for piecewise linear systems based on observability analysis that is in function of a commutation law related with the system's outpu. This virtual sensor is also known as a state estimator. Besides, it presents a detector of active mode when the commutation sequences of each linear subsystem are arbitrary and unknown. For the previous, this article proposes a set of virtual estimators that discern the commutation paths of the system and allow estimating their output. In this work a methodology in order to test the observability for piecewise linear systems with discrete time is proposed. An academic example is presented to show the obtained results. PMID:23447007

Assessing the spatial variability of mountain precipitation in California's Sierra Nevada using the Airborne Snow Observatory

NASA Astrophysics Data System (ADS)

Brandt, T.; Deems, J. S.; Painter, T. H.; Dozier, J.

2016-12-01

In California's Sierra Nevada, 10 or fewer winter storms are responsible for most of the annual precipitation, which falls mostly as snow. Presently, surface stations are used to measure the dynamics of mountain precipitation. However, even in places like the Sierra Nevada—one of the most gauged regions in the world—the paucity of surface stations can lead to large errors in precipitation thereby biasing both total water year and short-term streamflow forecasts. Remotely sensed snow depth and water equivalent, at a time scale that resolves storms, might provide a novel solution to the problems of: (1) quantifying the spatial variability of mountain precipitation; and (2) assessing gridded precipitation products that are mostly based on surface station interpolation. NASA's Airborne Snow Observatory (ASO), an imaging spectrometer and LiDAR system, has measured snow in the Tuolumne River Basin in California's Sierra Nevada for the past four years, 2013-2016; and, measurements will continue. Principally, ASO monitors the progression of melt for water supply forecasting, nonetheless, a number of flights bracketed storms allowing for estimates of snow accumulation. In this study we examine a few of the ASO recorded storms to determine both the basin and subbasin orographic effect as well as the spatial patterns in total precipitation. We then compare these results to a number of gridded climate products and weather models including: Daymet, the Parameter-elevation Regressions on Independent Slopes Model (PRISM), the North American Land Data Assimilation System (NLDAS-2), and the Weather Research and Forecasting (WRF) model. Finally, to put each ASO recorded storm into context, we use a climatology produced from snow pillows and the North American Regional Reanalysis (NARR) for 2014-2016 to examine key accumulation events, and classify storms based on their integrated water vapor flux.

Telescope Automation and Remote Observing System (TAROS)

NASA Astrophysics Data System (ADS)

Wilson, G.; Czezowski, A.; Hovey, G. R.; Jarnyk, M. A.; Nielsen, J.; Roberts, B.; Sebo, K.; Smith, D.; Vaccarella, A.; Young, P.

2005-12-01

TAROS is a system that will allow for the Australian National University telescopes at a remote location to be operated automatically or interactively with authenticated control via the internet. TAROS is operated by a Java front-end GUI and employs the use of several Java technologies - such as Java Message Service (JMS) for communication between the telescope and the remote observer, Java Native Interface to integrate existing data acquisition software written in C++ (CICADA) with new Java programs and the JSky collection of Java GUI components for parts of the remote observer client. In this poster the design and implementation of TAROS is described.

ORAC: a modern observing system for UKIRT

NASA Astrophysics Data System (ADS)

Bridger, Alan; Wright, Gillian S.; Economou, Frossie; Tan, Min; Currie, Malcolm J.; Pickup, David A.; Adamson, Andrew J.; Rees, Nicholas P.; Purves, Maren; Kackley, Russell

2000-06-01

The steady improvement in telescope performance at UKIRT and the increase in data acquisition rates led to a strong desired for an integrated observing framework that would meet the needs of future instrumentation, as well as providing some support for existing instrumentation. Thus the Observatory Reduction and Acquisition Control (ORAC) project was created in 1997 with the goals of improving the scientific productivity in the telescope, reducing the overall ongoing support requirements, and eventually supporting the use of more flexibly scheduled observing. The project was also expected to achieve this within a tight resource allocation. In October 1999 the ORAC system was commissioned at the United Kingdom Infrared Telescope.

The NOAA Satellite Observing System Architecture Study

NASA Technical Reports Server (NTRS)

Volz, Stephen; Maier, Mark; Di Pietro, David

2016-01-01

NOAA is beginning a study, the NOAA Satellite Observing System Architecture (NSOSA) study, to plan for the future operational environmental satellite system that will follow GOES and JPSS, beginning about 2030. This is an opportunity to design a modern architecture with no pre-conceived notions regarding instruments, platforms, orbits, etc. The NSOSA study will develop and evaluate architecture alternatives to include partner and commercial alternatives that are likely to become available. The objectives will include both functional needs and strategic characteristics (e.g., flexibility, responsiveness, sustainability). Part of this study is the Space Platform Requirements Working Group (SPRWG), which is being commissioned by NESDIS. The SPRWG is charged to assess new or existing user needs and to provide relative priorities for observational needs in the context of the future architecture. SPRWG results will serve as input to the process for new foundational (Level 0 and Level 1) requirements for the next generation of NOAA satellites that follow the GOES-R, JPSS, DSCOVR, Jason-3, and COSMIC-2 missions.

Defining the Canary Islands Oceanic Platform (PLOCAN) Observing System mission

NASA Astrophysics Data System (ADS)

Delory, Eric; Hernández-Brito, Joaquín.; Llínas, Octavio

2010-05-01

A permanent multidisciplinary ocean observing system is planned as both a technological and scientific infrastructure for the Canary Islands Oceanic Platform (PLOCAN). The first component of its two-fold mission is to respond to systems and processes' in-situ environmental testing, certification and benchmarking requirements. This will generally take place in dedicated oceanic experimental areas, from the vicinity of the platform to the deep ocean. While these areas and related infrastructures still are at definition stage, an anticipated prerequisite is that testbed observing assets will have to provide a broad range of measurements in agreement with, as well as in contribution to, current and upcoming environmental and technical standards. The second component is to contribute to the global effort towards continuous and real-time multidisciplinary ocean observations. Related activities will encompass climate change parameters characterization as well as important regional specificities like the preservation and study of the region's unique marine biodiversity and sparsely explored seabed. Continuous sampling is planned to progressively expand from the platform vicinity down to the end of the continental slope - to about 3000m depth, the surrounding seabed and water column, then scale up to the region, through mobile systems and fixed open-ocean stations. Such a large and diverse spectrum of observing activities stems from the fact that the PLOCAN observing system is at the center of a long-term strategy, thus granting the opportunity to plan its mission by way of an ambitious set of ocean measurement methods and technologies.

The European Marine Observing Network and the development of an Integrated European Ocean Observing System. An EuroGOOS perspective

NASA Astrophysics Data System (ADS)

Fernandez, Vicente; Gorringe, Patrick; Nolan, Glenn

2016-04-01

The ocean benefits many sectors of society, being the biggest reservoir of heat, water, carbon and oxygen and playing a fundamental role regulating the earth's climate. We rely on the oceans for food, transport, energy and recreation. Therefore, a sustained marine observation network is crucial to further our understanding of the oceanic environment and to supply scientific data to meet society's need. Marine data and observations in Europe, collected primarily by state governmental agencies, is offered via five Regional Operational Oceanographic Systems (ROOS) within the context of EuroGOOS (http://www.eurogos.eu), an International Non-Profit Association of national governmental agencies and research organizations (40 members from 19 member states) committed to European-scale operational oceanography within the context of the Intergovernmental Global Ocean Observing System (GOOS). Strong cooperation within these regions, enabling the involvement of additional partners and countries, forms the basis of EuroGOOS work. Ocean data collected from different type of sensors (e.g. moored buoys, tide gauges, Ferrybox systems, High Frequency radars, gliders and profiling floats) is accessible to scientist and other end users through data portals and initiatives such as the European Marine Observations and Data Network (EMODnet) (www.emodnet.eu) and the Copernicus Marine Service Copernicus (www.copernicus.eu). Although a relatively mature European ocean observing capability already exists and its well-coordinated at European level, some gaps have been identified, for example the demand for ecosystem products and services, or the case that biogeochemical observations are still relatively sparse particularly in coastal and shelf seas. Assessing gaps based on the capacity of the observing system to answer key societal challenges e.g. site suitability for aquaculture and ocean energy, oil spill response and contextual oceanographic products for fisheries and ecosystems is still

Mission operations concepts for Earth Observing System (EOS)

NASA Technical Reports Server (NTRS)

Kelly, Angelita C.; Taylor, Thomas D.; Hawkins, Frederick J.

1991-01-01

Mission operation concepts are described which are being used to evaluate and influence space and ground system designs and architectures with the goal of achieving successful, efficient, and cost-effective Earth Observing System (EOS) operations. Emphasis is given to the general characteristics and concepts developed for the EOS Space Measurement System, which uses a new series of polar-orbiting observatories. Data rates are given for various instruments. Some of the operations concepts which require a total system view are also examined, including command operations, data processing, data accountability, data archival, prelaunch testing and readiness, launch, performance monitoring and assessment, contingency operations, flight software maintenance, and security.

Ground-based Observation System Development for the Moon Hyper-spectral Imaging

NASA Astrophysics Data System (ADS)

Wang, Yang; Huang, Yu; Wang, Shurong; Li, Zhanfeng; Zhang, Zihui; Hu, Xiuqing; Zhang, Peng

2017-05-01

The Moon provides a suitable radiance source for on-orbit calibration of space-borne optical instruments. A ground-based observation system dedicated to the hyper-spectral radiometry of the Moon has been developed for improving and validating the current lunar model. The observation instrument using a dispersive imaging spectrometer is particularly designed for high-accuracy observations of the lunar radiance. The simulation and analysis of the push-broom mechanism is made in detail for lunar observations, and the automated tracking and scanning is well accomplished in different observational condition. A three-month series of hyper-spectral imaging experiments of the Moon have been performed in the wavelength range from 400 to 1000 nm near Lijiang Observatory (Yunnan, China) at phase angles -83°-87°. Preliminary results and data comparison are presented, and it shows the instrument performance and lunar observation capability of this system are well validated. Beyond previous measurements, this observation system provides the entire lunar disk images of continuous spectral coverage by adopting the push-broom mode with special scanning scheme and leads to the further research of lunar photometric model.

Developing Vocabularies to Improve Understanding and Use of NOAA Observing Systems

NASA Astrophysics Data System (ADS)

Austin, M.

2014-12-01

The NOAA Observing System Integrated Analysis project (NOSIA II), is an attempt to capture and tell the story of how valuable observing systems are in producing products and services that are required to fulfill the NOAA's diverse mission. NOAA's goals and mission areas cover a broad range of environmental data; a complexity exists in terms and vocabulary as applied to the creation of observing system derived products. The NOSIA data collection focused first on decomposing NOAA's goals in the creation and acceptance of Mission Service Areas (MSAs) by NOAA senior leadership. Products and services that supported the MSAs were then identified through the process of interviewing product producers across NOAA organization. Product Data inputs including models, databases and observing system were also identified. The NOSIA model contains over 20,000 nodes each representing levels in a network connecting products, datasources, users and desired outcomes. An immediate need became apparent that the complexity and variety of the data collected required data management to mature the quality and the content of the NOSIA model. The NOSIA Analysis Database (ADB) was developed initially to improve consistency of terms and data types to allow for the linkage of observing systems, products and NOAA's Goals and mission. The ADB also allowed for the prototyping of reports and product generation in an easily accessible and comprehensive format for the first time. Web based visualization of relationships between products, datasources, users, producers were generated to make the information easily understood This includes developing ontologies/vocabularies that are used for the development of users type specific products for NOAA leadership, Observing System Portfolio mangers and the users of NOAA data.

Spectroradiometric considerations for advanced land observing systems

NASA Technical Reports Server (NTRS)

Slater, P. N.

1986-01-01

Research aimed at improving the inflight absolute radiometric calibration of advanced land observing systems was initiated. Emphasis was on the satellite sensor calibration program at White Sands. Topics addressed include: absolute radiometric calibration of advanced remote sensing; atmospheric effects on reflected radiation; inflight radiometric calibration; field radiometric methods for reflectance and atmospheric measurement; and calibration of field relectance radiometers.

Observing Arctic Ecology using Networked Infomechanical Systems

NASA Astrophysics Data System (ADS)

Healey, N. C.; Oberbauer, S. F.; Hollister, R. D.; Tweedie, C. E.; Welker, J. M.; Gould, W. A.

2012-12-01

Understanding ecological dynamics is important for investigation into the potential impacts of climate change in the Arctic. Established in the early 1990's, the International Tundra Experiment (ITEX) began observational inquiry of plant phenology, plant growth, community composition, and ecosystem properties as part of a greater effort to study changes across the Arctic. Unfortunately, these observations are labor intensive and time consuming, greatly limiting their frequency and spatial coverage. We have expanded the capability of ITEX to analyze ecological phenomenon with improved spatial and temporal resolution through the use of Networked Infomechanical Systems (NIMS) as part of the Arctic Observing Network (AON) program. The systems exhibit customizable infrastructure that supports a high level of versatility in sensor arrays in combination with information technology that allows for adaptable configurations to numerous environmental observation applications. We observe stereo and static time-lapse photography, air and surface temperature, incoming and outgoing long and short wave radiation, net radiation, and hyperspectral reflectance that provides critical information to understanding how vegetation in the Arctic is responding to ambient climate conditions. These measurements are conducted concurrent with ongoing manual measurements using ITEX protocols. Our NIMS travels at a rate of three centimeters per second while suspended on steel cables that are ~1 m from the surface spanning transects ~50 m in length. The transects are located to span soil moisture gradients across a variety of land cover types including dry heath, moist acidic tussock tundra, shrub tundra, wet meadows, dry meadows, and water tracks. We have deployed NIMS at four locations on the North Slope of Alaska, USA associated with 1 km2 ARCSS vegetation study grids including Barrow, Atqasuk, Toolik Lake, and Imnavait Creek. A fifth system has been deployed in Thule, Greenland beginning in

Use of In-Situ and Remotely Sensed Snow Observations for the National Water Model in Both an Analysis and Calibration Framework.

NASA Astrophysics Data System (ADS)

Karsten, L. R.; Gochis, D.; Dugger, A. L.; McCreight, J. L.; Barlage, M. J.; Fall, G. M.; Olheiser, C.

2017-12-01

Since version 1.0 of the National Water Model (NWM) has gone operational in Summer 2016, several upgrades to the model have occurred to improve hydrologic prediction for the continental United States. Version 1.1 of the NWM (Spring 2017) includes upgrades to parameter datasets impacting land surface hydrologic processes. These parameter datasets were upgraded using an automated calibration workflow that utilizes the Dynamic Data Search (DDS) algorithm to adjust parameter values using observed streamflow. As such, these upgrades to parameter values took advantage of various observations collected for snow analysis. In particular, in-situ SNOTEL observations in the Western US, volunteer in-situ observations across the entire US, gamma-derived snow water equivalent (SWE) observations courtesy of the NWS NOAA Corps program, gridded snow depth and SWE products from the Jet Propulsion Laboratory (JPL) Airborne Snow Observatory (ASO), gridded remotely sensed satellite-based snow products (MODIS,AMSR2,VIIRS,ATMS), and gridded SWE from the NWS Snow Data Assimilation System (SNODAS). This study explores the use of these observations to quantify NWM error and improvements from version 1.0 to version 1.1, along with subsequent work since then. In addition, this study explores the use of snow observations for use within the automated calibration workflow. Gridded parameter fields impacting the accumulation and ablation of snow states in the NWM were adjusted and calibrated using gridded remotely sensed snow states, SNODAS products, and in-situ snow observations. This calibration adjustment took place over various ecological regions in snow-dominated parts of the US for a retrospective period of time to capture a variety of climatological conditions. Specifically, the latest calibrated parameters impacting streamflow were held constant and only parameters impacting snow physics were tuned using snow observations and analysis. The adjusted parameter datasets were then used to

US Integrated Ocean Observing System (IOOS°): Delivering Benefits to Science and Society

NASA Astrophysics Data System (ADS)

Willis, Z. S.

2011-12-01

The United States Integrated Ocean Observing System (IOOS°) is a user-driven, coordinated network of people, organizations, and technology that generate and disseminate continuous data about our coastal waters, Great Lakes, and oceans supported by strong research and development activities. IOOS° is our Eyes on our Oceans, Coasts and Great Lakes that enable the United States to track, predict, manage, and adapt to changes in our marine environment and deliver critical information to decision makers to improve safety, enhance our economy and protect our environment. IOOS provides a major shift in the approach to ocean observing by drawing together the vast network of disparate federal and non-federal observing systems to produce a cohesive suite of data, information, and products on a sufficient geographic and temporal scale to support decision-making. Two interdependent components constitute the U.S. IOOS: (1) the global ocean component, and (2) the coastal component. The strength of IOOS is in its partnerships, starting with the federal agencies, the partnerships extend internationally for the global component and to the local level for the coastal component. The coastal component includes the national set of observations for the U.S. Ocean, Coasts and Great Lakes, a network of Regional Associations that are establishing Regional Coastal Ocean Observing Systems (RCOOS) and the Alliance for Coastal Technologies (ACT). The U.S. IOOS is our nation's contribution to the Global Ocean Observing System (GOOS) - the ocean component of the Global Earth Observation System of Systems (GEOSS).

Through the Looking Glass: Droughtorama to Snowpocalypse in the Sierra Nevada as studied with the NASA Airborne Snow Observatory

NASA Astrophysics Data System (ADS)

Painter, T. H.; Bormann, K.; Deems, J. S.; Hedrick, A. R.; Marks, D. G.; Skiles, M.; Stock, G. M.

2017-12-01

Across the last five years, the Sierra Nevada has seen increasing drought and then an abrupt return to a top five snowpack. Fortunately, the NASA Airborne Snow Observatory has been flying the Central Sierra Nevada since the spring of 2013, quantifying critical mountain basins' snow water equivalent and snow albedo. The huge variation of snowpack years captured by the NASA ASO is of enormous benefit to water cycle science, ecosystem science, and water management utilization of ASO data and its modeling. It allows a much broader understanding of mountain basin snow season cases for understanding snowmelt runoff, snow/rain mixes, snowfall distribution, evapotranspiration, soil moisture, and glacier mass balance. For water management, trust in empirical and physically-based modeling from the ASO data for application anywhere in the range of snow years is greatly improved by having consistency in that modeling with the span of years ASO has characterized. The NASA ASO was designed to characterize mountain snowpack and fill this void in water cycle science. Our original conversations with partner California Department of Water Resources in 2011 focused on the utility of ASO for flood risk mitigation, given the large snowfall of that year. However, from 2012 through 2016, California snowpacks expressed horrible drought, reaching the nadir in 2015 with the lowest snowpack on record. The 2016 snowpack was nearly normal according to snow pillows and snow courses (ASO's record is too short to define a `normal' year). However, 2017 had enormous snowfall in January and February, keeping snow pillows on track with the largest year on record, 1982-83. However, March backed off and the record year was lost. Still, accumulation was enormous. In parts of the San Joaquin basin, snow depths were > 30 m. The sum of near April 1 ASO total basin SWE for 2013 through 2016 in the Tuolumne Basin was only 92% of the near April 1, 2017 acquisition. In addition to the large accumulation of

XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hori, Shin-Ichiro; Yamamoto, Tsuyoshi; Obika, Satoshi, E-mail: obika@phs.osaka-u.ac.jp

Antisense oligonucleotides (ASOs) can suppress the expression of a target gene by cleaving pre-mRNA and/or mature mRNA via RNase H1. Following the initial endonucleolytic cleavage by RNase H1, the target RNAs are degraded by a mechanism that is poorly understood. To better understand this degradation pathway, we depleted the expression of two major 5′ to 3′ exoribonucleases (XRNs), named XRN1 and XRN2, and analyzed the levels of 3′ fragments of the target RNAs in vitro. We found that the 3′ fragments of target pre-mRNA generated by ASO were almost completely degraded from their 5′ ends by nuclear XRN2 after RNase H1-mediatedmore » cleavage, whereas the 3′ fragments of mature mRNA were partially degraded by XRN2. In contrast to ASO, small interference RNA (siRNA) could reduce the expression level of only mature mRNA, and the 3′ fragment was degraded by cytoplasmic XRN1. Our findings indicate that the RNAs targeted by RNase H1-dependent ASO are rapidly degraded in the nucleus, contrary to the cytoplasmic degradation pathway mediated by siRNA. - Highlights: • We compared the degradation mechanism of the transcript targeted by ASO and siRNA. • We focused on two 5′ to 3′ exoribonucleases, cytoplasmic XRN1, and nuclear XRN2. • The 3′ fragment of target pre-mRNA generated by ASO was degraded by XRN2. • The 3′ fragment of target mRNA generated by ASO was partially degraded by XRN2. • XRN1 depletion promoted accumulation of the 3′ fragment of mRNA generated by siRNA.« less

Validating SWE reconstruction using Airborne Snow Observatory measurements in the Sierra Nevada

NASA Astrophysics Data System (ADS)

Bair, N.; Rittger, K.; Davis, R. E.; Dozier, J.

2015-12-01

The Airborne Snow Observatory (ASO) program offers high resolution estimates of snow water equivalent (SWE) in several small basins across California during the melt season. Primarily, water managers use this information to model snowmelt runoff into reservoirs. Another, and potentially more impactful, use of ASO SWE measurements is in validating and improving satellite-based SWE estimates which can be used in austere regions with no ground-based snow or water measurements, such as Afghanistan's Hindu Kush. Using the entire ASO dataset to date (2013-2015) which is mostly from the Upper Tuolumne basin, but also includes measurements from 2015 in the Kings, Rush Creek, Merced, and Mammoth Lakes basins, we compare ASO measurements to those from a SWE reconstruction method. Briefly, SWE reconstruction involves downscaling energy balance forcings to compute potential melt energy, then using satellite-derived estimates of fractional snow covered area (fSCA) to estimate snow melt from potential melt. The snowpack can then be built in reverse, given a remotely-sensed date of snow disappearance (fSCA=0). Our model has improvements over previous iterations in that it: uses the full energy balance (compared to a modified degree-day) approach, models bulk and surface snow temperatures, accounts for ephemeral snow, and uses a remotely-sensed snow albedo adjusted for impurities. To check that ASO provides accurate snow measurements, we compare fSCA derived from ASO snow depth at 3 m resolution with fSCA from a spectral unmixing algorithm for LandSAT at 30 m, and from binary SCA estimates from Geoeye at 0.5 m from supervised classification. To conclude, we document how our reconstruction model has evolved over the years and provide specific examples where improvements have been made using ASO and other verification sources.

The Southern California Coastal Ocean Observing System (SCCOOS): Developing A Coastal Observation System To Enable Both Science Based Decision Making And Scientific Discovery

NASA Astrophysics Data System (ADS)

Terrill, E.; John, O.

2005-05-01

The Southern California Coastal Ocean Observing System (SCCOOS) is a consortium that extends from Northern Baja CA in Mexico to Morro Bay at the southern edge of central California, and aims to streamline, coordinate, and further develop individual institutional efforts by creating an integrated, multidisciplinary coastal observatory in the Bight of Southern California for the benefit of society. By leveraging existing infrastructure, partnerships, and private, local, state, and federal resources, SCCOOS is developing a fully operational coastal observation system to address issues related to coastal water quality, marine life resources, and coastal hazards for end user communities spanning local, state, and federal interests. However, to establish a sensible observational approach to address these societal drivers, sound scientific approaches are required in both the system design and the transformation of data to useful products. Since IOOS and coastal components of the NSF Ocean Observatories Initiative (OOI) are not mutually exclusive within this framework, the SCCOOS consortium of observatory implementers have created an organizational structure that encourages dovetailing of OOI into the routine observations provided by the operational components of a regional IOOS. To begin the development, SCCOOS has grant funding from the California Coastal Conservancy as part of a $21M, statewide initiative to establish a Coastal Ocean Currents Monitoring Program, and funding from NOAA's Coastal Observing Technology System (COTS). In addition, SCCOOS is leveraging IT development that has been supported by the NSF Information Technology Research program Real-time observatories, Applications,and Data Manageemnt Network (ROADNET), and anticipates using developments which will result from the NSF Laboratory for Ocean Observatory Knowledge Integration Grid (LOOKING) program. The observational components now funded at SCCOOS include surface current mapping by HF radar; high

Hippocampal UCP2 is essential for cognition and resistance to anxiety but not required for the benefits of exercise.

PubMed

Wang, D; Zhai, X; Chen, P; Yang, M; Zhao, J; Dong, J; Liu, H

2014-09-26

Uncoupling protein-2 (UCP2) reduces oxidative stress by facilitating the influx of protons into mitochondrial matrix, thus dissociating mitochondrial oxidation from ATP synthesis. UCP2 is expressed abundantly in brain areas and plays a key role in neuroprotection. Here, we sought to determine if UCP2 deficiency produces cognitive impairment and anxiety in young mice, and to determine if hippocampal UCP2 is essential for the beneficial effects of voluntary exercise. Antisense oligonucleotide (ASO) was used to produce UCP2 knockdown in mice. Our results firstly showed that UCP2-targeted ASO significantly reduced UCP2 mRNA and protein expression in the hippocampus. ASO treatment impaired learning and memory of the mice in Y-maze, T-maze, and object recognition tests (ORT). ASO-treated mice exhibited more anxiously in OPT, light/dark box test, and elevated plus maze (EPM) than the control mice. We also found that wheel running ameliorated cognitive dysfunction and anxiety-like behaviors in ASO-treated mice. Furthermore, voluntary exercise reversed ASO-induced changes in hippocampal levels of serotonin (5-HT), dopamine (DA), and norepinephrine (NE). However, UCP2 protein in the hippocampus was not correlated with cognitive and anxiolytic benefits of exercise. These findings suggest that hippocampal UCP2 is essential for cognitive function and the resistance to anxiety of mice, but not required for the beneficial effects of exercise. Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

Antisense Masking of an hnRNP A1/A2 Intronic Splicing Silencer Corrects SMN2 Splicing in Transgenic Mice

PubMed Central

Hua, Yimin; Vickers, Timothy A.; Okunola, Hazeem L.; Bennett, C. Frank; Krainer, Adrian R.

2008-01-01

survival of motor neuron 2, centromeric (SMN2) is a gene that modifies the severity of spinal muscular atrophy (SMA), a motor-neuron disease that is the leading genetic cause of infant mortality. Increasing inclusion of SMN2 exon 7, which is predominantly skipped, holds promise to treat or possibly cure SMA; one practical strategy is the disruption of splicing silencers that impair exon 7 recognition. By using an antisense oligonucleotide (ASO)-tiling method, we systematically screened the proximal intronic regions flanking exon 7 and identified two intronic splicing silencers (ISSs): one in intron 6 and a recently described one in intron 7. We analyzed the intron 7 ISS by mutagenesis, coupled with splicing assays, RNA-affinity chromatography, and protein overexpression, and found two tandem hnRNP A1/A2 motifs within the ISS that are responsible for its inhibitory character. Mutations in these two motifs, or ASOs that block them, promote very efficient exon 7 inclusion. We screened 31 ASOs in this region and selected two optimal ones to test in human SMN2 transgenic mice. Both ASOs strongly increased hSMN2 exon 7 inclusion in the liver and kidney of the transgenic animals. Our results show that the high-resolution ASO-tiling approach can identify cis-elements that modulate splicing positively or negatively. Most importantly, our results highlight the therapeutic potential of some of these ASOs in the context of SMA. PMID:18371932

Rescue of Outer Hair Cells with Antisense Oligonucleotides in Usher Mice Is Dependent on Age of Treatment.

PubMed

Ponnath, Abhilash; Depreux, Frederic F; Jodelka, Francine M; Rigo, Frank; Farris, Hamilton E; Hastings, Michelle L; Lentz, Jennifer J

2018-02-01

The absence of functional outer hair cells is a component of several forms of hereditary hearing impairment, including Usher syndrome, the most common cause of concurrent hearing and vision loss. Antisense oligonucleotide (ASO) treatment of mice with the human Usher mutation, Ush1c c.216G>A, corrects gene expression and significantly improves hearing, as measured by auditory-evoked brainstem responses (ABRs), as well as inner and outer hair cell (IHC and OHC) bundle morphology. However, it is not clear whether the improvement in hearing achieved by ASO treatment involves the functional rescue of outer hair cells. Here, we show that Ush1c c.216AA mice lack OHC function as evidenced by the absence of distortion product otoacoustic emissions (DPOAEs) in response to low-, mid-, and high-frequency tone pairs. This OHC deficit is rescued by treatment with an ASO that corrects expression of Ush1c c.216G>A. Interestingly, although rescue of inner hairs cells, as measured by ABR, is achieved by ASO treatment as late as 7 days after birth, rescue of outer hair cells, measured by DPOAE, requires treatment before post-natal day 5. These results suggest that ASO-mediated rescue of both IHC and OHC function is age dependent and that the treatment window is different for the different cell types. The timing of treatment for congenital hearing disorders is of critical importance for the development of drugs such ASO-29 for hearing rescue.

AsyncStageOut: Distributed User Data Management for CMS Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Riahi, H.; Wildish, T.; Ciangottini, D.

2015-12-23

AsyncStageOut (ASO) is a new component of the distributed data analysis system of CMS, CRAB, designed for managing users' data. It addresses a major weakness of the previous model, namely that mass storage of output data was part of the job execution resulting in inefficient use of job slots and an unacceptable failure rate at the end of the jobs. ASO foresees the management of up to 400k files per day of various sizes, spread worldwide across more than 60 sites. It must handle up to 1000 individual users per month, and work with minimal delay. This creates challenging requirementsmore » for system scalability, performance and monitoring. ASO uses FTS to schedule and execute the transfers between the storage elements of the source and destination sites. It has evolved from a limited prototype to a highly adaptable service, which manages and monitors the user file placement and bookkeeping. To ensure system scalability and data monitoring, it employs new technologies such as a NoSQL database and re-uses existing components of PhEDEx and the FTS Dashboard. We present the asynchronous stage-out strategy and the architecture of the solution we implemented to deal with those issues and challenges. The deployment model for the high availability and scalability of the service is discussed. The performance of the system during the commissioning and the first phase of production are also shown, along with results from simulations designed to explore the limits of scalability.« less

Newer systems for bacterial resistances to toxic heavy metals.

PubMed Central

Silver, S; Ji, G

1994-01-01

Bacterial plasmids contain specific genes for resistances to toxic heavy metal ions including Ag+, AsO2-, AsO4(3-), Cd2+, Co2+, CrO4(2-), Cu2+, Hg2+, Ni2+, Pb2+, Sb3+, and Zn2+. Recent progress with plasmid copper-resistance systems in Escherichia coli and Pseudomonas syringae show a system of four gene products, an inner membrane protein (PcoD), an outer membrane protein (PcoB), and two periplasmic Cu(2+)-binding proteins (PcoA and PcoC). Synthesis of this system is governed by two regulatory proteins (the membrane sensor PcoS and the soluble responder PcoR, probably a DNA-binding protein), homologous to other bacterial two-component regulatory systems. Chromosomally encoded Cu2+ P-type ATPases have recently been recognized in Enterococcus hirae and these are closely homologous to the bacterial cadmium efflux ATPase and the human copper-deficiency disease Menkes gene product. The Cd(2+)-efflux ATPase of gram-positive bacteria is a large P-type ATPase, homologous to the muscle Ca2+ ATPase and the Na+/K+ ATPases of animals. The arsenic-resistance system of gram-negative bacteria functions as an oxyanion efflux ATPase for arsenite and presumably antimonite. However, the structure of the arsenic ATPase is fundamentally different from that of P-type ATPases. The absence of the arsA gene (for the ATPase subunit) in gram-positive bacteria raises questions of energy-coupling for arsenite efflux. The ArsC protein product of the arsenic-resistance operons of both gram-positive and gram-negative bacteria is an intracellular enzyme that reduces arsenate [As(V)] to arsenite [As(III)], the substrate for the transport pump. Newly studied cation efflux systems for Cd2+, Zn2+, and Co2+ (Czc) or Co2+ and Ni2+ resistance (Cnr) lack ATPase motifs in their predicted polypeptide sequences. Therefore, not all plasmid-resistance systems that function through toxic ion efflux are ATPases. The first well-defined bacterial metallothionein was found in the cyanobacterium Synechococcus

Detecting climate variations and change: New challenges for observing and data management systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karl, T.R.; Quayle, R.G.; Groisman, P.Ya.

1993-08-01

Several essential aspects of weather observing and the management of weather data related to improving knowledge of climate variations and change in the surface boundary layer and the consequences for socioeconomic and biogeophysical systems, are discussed. The issues include long-term homogeneous time series of routine weather observations; time- and space-scale resolution of datasets derived from the observations; information about observing systems, data collection systems, and data reduction algorithms; and the enhancement of weather observing systems to serve as climate observing systems. Although much has been learned from existing weather networks and methods of data management, the system is far frommore » perfect. Several vital areas have not received adequate attention. Particular improvements are needed in the interaction between network designers and climatologists; operational analyses that focus on detecting and documenting outliers and time-dependent biases within datasets; developing the means to cope with and minimize potential inhomogeneities in weather observing systems; and authoritative documentation of how various aspects of climate have or have not changed. In this last area, close attention must be given to time and space resolution of the data. In many instances the time and space resolution requirements for understanding why the climate changes are not synonymous with understanding how it has changed or varied. This is particularly true within the surface boundary layer. A standard global daily/monthly climate message should also be introduced to supplement current Global Telecommunication System's CLIMAT data. Overall, a call is made for improvements in routine weather observing, data management, and analysis systems. Routine observations have provided (and will continue to provide) most of the information regarding how the climate has changed during the last 100 years affecting where we live, work, and grow our food. 58 refs., 8 figs

LBT observations of the HR8799 planetary system

NASA Astrophysics Data System (ADS)

Mesa, D.; Arcidiacono, C.; Claudi, R. U.; Desidera, S.; Esposito, S.; Gratton, R.; Masciadri, E.

2013-09-01

We present here observations of the HR8799 planetary system performed in H and Ks band exploiting the AO system at the Large Binocular Telescope and the PISCES camera. Thanks to the excellent performence of the instrument we were able to detect for the first time the inner known planet of the system (HR8799) in the H band. Precise photometric and astrometric measures have been taken for all the four planets. Further, exploiting ours and previous astrometric results, we were able to put some limits on the planetary orbits of the four planets. The analysis of the dinamical stability of the system seems to show lower planetary masses than the ones adopted until now.

Safety of antisense oligonucleotide and siRNA-based therapeutics.

PubMed

Chi, Xuan; Gatti, Philip; Papoian, Thomas

2017-05-01

Oligonucleotide-based therapy is an active area of drug development designed to treat a variety of gene-specific diseases. Two of the more promising platforms are the antisense oligonucleotides (ASOs) and short interfering RNAs (siRNAs), both of which are often directed against similar targets. In light of recent reports on clinical trials of severe thrombocytopenia with two different ASO drugs and increased peripheral neuropathy with an siRNA drug, we compared and contrasted the specific safety characteristics of these two classes of oligonucleotide therapeutic. The objectives were to assess factors that could contribute to the specific toxicities observed with these two classes of promising drugs, and get a better understanding of the potential mechanism(s) responsible for these rare, but serious, adverse events. Published by Elsevier Ltd.

Using the SPICE system to help plan and interpret space science observations

NASA Technical Reports Server (NTRS)

Acton, Charles H., Jr.

1993-01-01

A portable multimission information system named SPICE is used to assemble, archive, and provide easy user access to viewing geometry and other ancillary information needed by space scientists to interpret observations of bodies within our solar system. The modular nature of this system lends it to use in planning such observations as well. With a successful proof of concept on Voyager, the SPICE system has been adapted to the Magellan, Galileo and Mars Observer missions, and to a variety of ground based operations. Adaptation of SPICE for Cassini and the Russian Mars 94/96 projects is underway, and work on Cassini will follow, SPICE has been used to support observation planning for moving targets on the Hubble Space Telescope Project. Applications for SPICE on earth science, space physics and other astrophysics missions are under consideration.

Observers for Systems with Nonlinearities Satisfying an Incremental Quadratic Inequality

NASA Technical Reports Server (NTRS)

Acikmese, Ahmet Behcet; Corless, Martin

2004-01-01

We consider the problem of state estimation for nonlinear time-varying systems whose nonlinearities satisfy an incremental quadratic inequality. These observer results unifies earlier results in the literature; and extend it to some additional classes of nonlinearities. Observers are presented which guarantee that the state estimation error exponentially converges to zero. Observer design involves solving linear matrix inequalities for the observer gain matrices. Results are illustrated by application to a simple model of an underwater.

A Conceptual Framework for Assessment of the Benefits of a Global Earth Observation System of Systems

NASA Astrophysics Data System (ADS)

Fritz, S.; Scholes, R. J.; Obersteiner, M.; Bouma, J.

2007-12-01

The aim of the Global Earth Observation System of Systems (GEOSS) is to contribute to human wellbeing though improving the information available to decision-makers at all levels relating to human health and safety, protection of the global environment, the reduction of losses from natural disasters, and achieving sustainable development. Specifically, GEOSS proposes that better international co-operation in the collection, interpretation and sharing of Earth Observation information is an important and cost-effective mechanism for achieving this aim. While there is a widespread intuition that this proposition is correct, at some point the following question needs to be answered: how much additional investment in Earth Observation (and specifically, in its international integration) is enough? This leads directly to some challenging subsidiary questions, such as how can the benefits of Earth Observation be assessed? What are the incremental costs of GEOSS? Are there societal benefit areas where the return on investment is higher than in others? The Geo-Bene project has developed a `benefit chain' concept as a framework for addressing these questions. The basic idea is that an incremental improvement in the observing system (including its data collection, interpretation and information-sharing aspects) will result in an improvement in the quality of decisions based on that information. This will in turn lead to better societal outcomes, which have a value. This incremental value must be judged against the incremental cost of the improved observation system. Since in many cases there will be large uncertainties in the estimation of both the costs and the benefits, and it may not be possible to express one or both of them in monetary terms, we show how order-of-magnitude approaches and a qualitative understanding of the shape of the cost-benefit curves can help guide rational investment decision in Earth Observation systems.

The Earth Observation Data for Habitat Monitoring (EODHaM) system

NASA Astrophysics Data System (ADS)

Lucas, Richard; Blonda, Palma; Bunting, Peter; Jones, Gwawr; Inglada, Jordi; Arias, Marcela; Kosmidou, Vasiliki; Petrou, Zisis I.; Manakos, Ioannis; Adamo, Maria; Charnock, Rebecca; Tarantino, Cristina; Mücher, Caspar A.; Jongman, Rob H. G.; Kramer, Henk; Arvor, Damien; Honrado, Joāo Pradinho; Mairota, Paola

2015-05-01

To support decisions relating to the use and conservation of protected areas and surrounds, the EU-funded BIOdiversity multi-SOurce monitoring System: from Space TO Species (BIO_SOS) project has developed the Earth Observation Data for HAbitat Monitoring (EODHaM) system for consistent mapping and monitoring of biodiversity. The EODHaM approach has adopted the Food and Agriculture Organization Land Cover Classification System (LCCS) taxonomy and translates mapped classes to General Habitat Categories (GHCs) from which Annex I habitats (EU Habitats Directive) can be defined. The EODHaM system uses a combination of pixel and object-based procedures. The 1st and 2nd stages use earth observation (EO) data alone with expert knowledge to generate classes according to the LCCS taxonomy (Levels 1 to 3 and beyond). The 3rd stage translates the final LCCS classes into GHCs from which Annex I habitat type maps are derived. An additional module quantifies changes in the LCCS classes and their components, indices derived from earth observation, object sizes and dimensions and the translated habitat maps (i.e., GHCs or Annex I). Examples are provided of the application of EODHaM system elements to protected sites and their surrounds in Italy, Wales (UK), the Netherlands, Greece, Portugal and India.

Enhancement of observability and protection of smart power system

NASA Astrophysics Data System (ADS)

Siddique, Abdul Hasib

It is important for a modern power grid to be smarter in order to provide reliable and sustainable supply of electricity. Traditional way of receiving data from the wired system is a very old and outdated technology. For a quicker and better response from the electric system, it is important to look at wireless systems as a feasible option. In order to enhance the observability and protection it is important to integrate wireless technology with the modern power system. In this thesis, wireless network based architecture for wide area monitoring and an alternate method for performing current measurement for protection of generators and motors, has been adopted. There are basically two part of this project. First part deals with the wide area monitoring of the power system and the second part focuses more on application of wireless technology from the protection point of view. A number of wireless method have been adopted in both the part, these includes Zigbee, analog transmission (Both AM and FM) and digital transmission. The main aim of our project was to propose a cost effective wide area monitoring and protection method which will enhance the observability and stability of power grid. A new concept of wireless integration in the power protection system has been implemented in this thesis work.

Polarimetric Radar Retrievals in Southeast Texas During Hurricane Harvey

NASA Astrophysics Data System (ADS)

Wolff, D. B.; Petersen, W. A.; Tokay, A.; Marks, D. A.; Pippitt, J. L.; Kirstetter, P. E.

2017-12-01

Hurricane Harvey hit the Texas Gulf Coast as a major hurricane on August 25, 2017 before exiting the state as a tropical storm on September 1, 2017. In its wake, it left a flood of historic proportions, with some areas measuring 60 inches of rain over a five-day period. Although the storm center stayed west of the immediate Houston area training bands of precipitation impacted the Houston area for five full days. The National Weather Service (NWS) WSR88D dual-polarimetric radar (KHGX), located southeast of Houston, maintained operations for the entirety of the event. The Harris County Flood Warning System (HCFWS) had 150 rain gauges deployed in its network and seven NWS Automated Surface Observing Systems (ASOS) rain gauges are also located in the area. In this study, we used the full radar data set to retrieve daily and event-total precipitation estimates within 120 km of the KHGX radar for the period August 25-29, 2017. These estimates were then compared to the HCFWS and ASOS gauges. Three different polarimetric hybrid rainfall retrievals were used: Ciffeli et al. 2011; Bringi et al. 2004; and, Chen et al. 2017. Each of these hybrid retrievals have demonstrated robust performance in the past. However, both daily and event-total comparisons from each of these retrievals compared to those of HCFWS and ASOS rain gauge networks resulted in significant underestimates by the radar retrievals. These radar underestimates are concerning. Sources of error and variance will be investigated to understand the source of radar-gauge disagreement. One current hypothesis is that due to the large number of small drops often found in hurricanes, the differential reflectivity and specific differential phase are relatively small so that the hybrid algorithms use only the reflectivity/rain rate procedure (so called Z-R relationships), and hence rarely invoke the ZDR or KDP procedures. Thus, an alternative Z-R relationship must be invoked to retrieve accurate rain rate estimates.

ALMA and VLA observations of the HD 141569 system

NASA Astrophysics Data System (ADS)

White, Jacob Aaron; Boley, A. C.; MacGregor, M. A.; Hughes, A. M.; Wilner, D. J.

2018-03-01

We present VLA 9 mm (33 GHz) and archival ALMA 2.9 mm (103 GHz) observations of the HD 141569 system. The VLA observations achieve a resolution of 0.25 arcsec (˜28 au) and a sensitivity of 4.7 μJy beam- 1. We find (1) a 52 ± 5 μJy point source at the location of HD 141569A that shows potential variability, (2) the detected flux is contained within the SED-inferred central clearing of the disc meaning the spectral index of the dust disc is steeper than previously inferred, and (3) the M dwarf companions are also detected and variable. Previous lower resolution VLA observations (semester 14A) found a higher flux density, interpreted as solely dust emission. When combined with ALMA observations, the VLA 14A observations suggested the spectral index, and grain size distribution of HD 141569's disc was shallow and an outlier among debris systems. Using archival ALMA observations of HD 141569 at 0.87 and 2.9 mm, we find a dust spectral index of αmm = 1.81 ± 0.20. The VLA 16A flux corresponds to a brightness temperature of ˜5 × 106 K, suggesting strong non-disc emission is affecting the inferred grain properties. The VLA 16A flux density of the M2V companion HD 141569B is 149 ± 9 μJy, corresponding to a brightness temperature of ˜2 × 108 K and suggesting significant stellar variability when compared to the VLA14A observations, which are smaller by a factor of ˜6.

Full Two-Body Problem Mass Parameter Observability Explored Through Doubly Synchronous Systems

NASA Astrophysics Data System (ADS)

Davis, Alex Benjamin; Scheeres, Daniel

2018-04-01

The full two-body problem (F2BP) is often used to model binary asteroid systems, representing the bodies as two finite mass distributions whose dynamics are influenced by their mutual gravity potential. The emergent behavior of the F2BP is highly coupled translational and rotational mutual motion of the mass distributions. For these systems the doubly synchronous equilibrium occurs when both bodies are tidally-locked and in a circular co-orbit. Stable oscillations about this equilibrium can be shown, for the nonplanar system, to be combinations of seven fundamental frequencies of the system and the mutual orbit rate. The fundamental frequencies arise as the linear periods of center manifolds identified about the equilibrium which are heavily influenced by each body’s mass parameters. We leverage these eight dynamical constraints to investigate the observability of binary asteroid mass parameters via dynamical observations. This is accomplished by proving the nonsingularity of the relationship between the frequencies and mass parameters for doubly synchronous systems. Thus we can invert the relationship to show that given observations of the frequencies, we can solve for the mass parameters of a target system. In so doing we are able to predict the estimation covariance of the mass parameters based on observation quality and define necessary observation accuracies for desired mass parameter certainties. We apply these tools to 617 Patroclus, a doubly synchronous Trojan binary and flyby target of the LUCY mission, as well as the Pluto and Charon system in order to predict mutual behaviors of these doubly synchronous systems and to provide observational requirements for these systems’ mass parameters

An operational, multistate, earth observation data management system

NASA Technical Reports Server (NTRS)

Eastwood, L. F., Jr.; Hill, C. T.; Morgan, R. P.; Gohagan, J. K.; Hays, T. R.; Ballard, R. J.; Crnkovich, G. G.; Schaeffer, M. A.

1977-01-01

State, local, and regional agencies involved in natural resources management were investigated as potential users of satellite remotely sensed data. This group's needs are assessed and alternative data management systems serving some of those needs are outlined. It is concluded that an operational earth observation data management system will be of most use to these user agencies if it provides a full range of information services -- from raw data acquisition to interpretation and dissemination of final information products.

Towards a regional coastal ocean observing system: An initial design for the Southeast Coastal Ocean Observing Regional Association

NASA Astrophysics Data System (ADS)

Seim, H. E.; Fletcher, M.; Mooers, C. N. K.; Nelson, J. R.; Weisberg, R. H.

2009-05-01

A conceptual design for a southeast United States regional coastal ocean observing system (RCOOS) is built upon a partnership between institutions of the region and among elements of the academic, government and private sectors. This design envisions support of a broad range of applications (e.g., marine operations, natural hazards, and ecosystem-based management) through the routine operation of predictive models that utilize the system observations to ensure their validity. A distributed information management system enables information flow, and a centralized information hub serves to aggregate information regionally and distribute it as needed. A variety of observing assets are needed to satisfy model requirements. An initial distribution of assets is proposed that recognizes the physical structure and forcing in the southeast U.S. coastal ocean. In-situ data collection includes moorings, profilers and gliders to provide 3D, time-dependent sampling, HF radar and surface drifters for synoptic sampling of surface currents, and satellite remote sensing of surface ocean properties. Nested model systems are required to properly represent ocean conditions from the outer edge of the EEZ to the watersheds. An effective RCOOS will depend upon a vital "National Backbone" (federally supported) system of in situ and satellite observations, model products, and data management. This dependence highlights the needs for a clear definition of the National Backbone components and a Concept of Operations (CONOPS) that defines the roles, functions and interactions of regional and federal components of the integrated system. A preliminary CONOPS is offered for the Southeast (SE) RCOOS. Thorough system testing is advocated using a combination of application-specific and process-oriented experiments. Estimates of costs and personnel required as initial components of the SE RCOOS are included. Initial thoughts on the Research and Development program required to support the RCOOS are

El Nino and the Global Ocean Observing System

NASA Technical Reports Server (NTRS)

Halpern, David

1999-01-01

Until a decade ago, an often-quoted expression in oceanography is that very few observations are recorded throughout the ocean. Now, the sentiment is no longer valid in the uppermost 10% of the tropical Pacific Ocean nor at the surface of the global ocean. One of the remarkable legacies of the 1985-1994 Tropical Oceans Global Atmosphere (TOGA) Program is an in situ marine meteorological and upper oceanographic measurement array throughout the equatorial Pacific to monitor the development and maintenance of El Nino episodes. The TOGA Observing System, which initially consisted of moored- and drifting-buoy arrays, a network of commercial ships, and coastal and island stations, now includes a constellation of satellites and data-assimilating models to simulate subsurface oceanographic conditions. The El Nino and La Nina tropical Pacific Ocean observing system represents the initial phase of an integrated global ocean observing system. Remarkable improvements have been made in ocean model simulation of subsurface currents, but some problems persist. For example, the simulation of the South Equatorial Current (SEC) remains an important challenge in the 2S-2N Pacific equatorial wave guide. During El Nino the SEC at the equator is reduced and sometimes the direction is reversed, becoming eastward. Both conditions allow warm water stored in the western Pacific to invade the eastern region, creating an El Nino episode. Assimilation of data is a tenet of faith to correct simulation errors caused by deficiencies in surface fluxes (especially wind stress) and parameterizations of subgrid-scale physical processes. In the first of two numerical experiments, the Pacific SEC was simulated with and without assimilation of subsurface temperature data. Along the equator, a very weak SEC occurred throughout the eastern Pacific, independent of assimilation of data. However, as displayed in the diagram, in the western Pacific there was no satisfactory agreement between the two

Extreme AO Observations of Two Triple Asteroid Systems with SPHERE

NASA Astrophysics Data System (ADS)

Yang, B.; Wahhaj, Z.; Beauvalet, L.; Marchis, F.; Dumas, C.; Marsset, M.; Nielsen, E. L.; Vachier, F.

2016-04-01

We present the discovery of a new satellite of asteroid (130) Elektra—S/2014 (130) 1—in differential imaging and in integral field spectroscopy data over multiple epochs obtained with Spectro-Polarimetric High-contrast Exoplanet Research/Very Large Telescope. This new (second) moonlet of Elektra is about 2 km across, on an eccentric orbit, and about 500 km away from the primary. For a comparative study, we also observed another triple asteroid system, (93) Minerva. For both systems, component-resolved reflectance spectra of the satellites and primary were obtained simultaneously. No significant spectral difference was observed between the satellites and the primary for either triple system. We find that the moonlets in both systems are more likely to have been created by sub-disruptive impacts as opposed to having been captured.

Sliding mode control-based linear functional observers for discrete-time stochastic systems

NASA Astrophysics Data System (ADS)

Singh, Satnesh; Janardhanan, Sivaramakrishnan

2017-11-01

Sliding mode control (SMC) is one of the most popular techniques to stabilise linear discrete-time stochastic systems. However, application of SMC becomes difficult when the system states are not available for feedback. This paper presents a new approach to design a SMC-based functional observer for discrete-time stochastic systems. The functional observer is based on the Kronecker product approach. Existence conditions and stability analysis of the proposed observer are given. The control input is estimated by a novel linear functional observer. This approach leads to a non-switching type of control, thereby eliminating the fundamental cause of chatter. Furthermore, the functional observer is designed in such a way that the effect of process and measurement noise is minimised. Simulation example is given to illustrate and validate the proposed design method.

NIRCam Coronagraphic Observations of Disks and Planetary Systems

NASA Astrophysics Data System (ADS)

Beichman, Charles A.; Ygouf, Marie; Gaspar, Andras; NIRCam Science Team

2017-06-01

The NIRCam coronagraph offers a dramatic increase in sensitivity at wavelengths of 3-5 um where young planets are brightest. While large ground-based telescopes with Extreme Adaptive Optics have an advantage in inner working angle, NIRCam's sensitivity will allow high precision photometry for known planets and searches for planets with masses below that of Saturn. For debris disk science NIRCam observations will address the scattering properties of dust, look for evidence of ices and tholins, and search for planets which affect the structure of the disk itself.The NIRCam team's GTO program includes medium-band filter observations of known young planets having 1-5 Jupiter masses. A collaborative program with the MIRI team will provide coronagraphic observations at longer wavelengths. The combined dataset will yield the exoplanet’s total luminosity and effective temperature, an estimate of the initial entropy of the newly-formed planet, and the retrieval of atmospheric properties.The program will also make deep searches for lower mass planets toward known planetary systems, nearby young M stars and debris disk systems. Achievable mass limits range from ~1 Jupiter mass beyond 20 AU for the brightest A stars to perhaps a Uranus mass within 10 AU for the closest M stars.We will discuss details of the coronagraphic program for both the exoplanet and debris disk cases with an emphasis on using APT to optimize the observations of target and reference stars.

Ionospheric Simulation System for Satellite Observations and Global Assimilative Model Experiments - ISOGAME

NASA Technical Reports Server (NTRS)

Pi, Xiaoqing; Mannucci, Anthony J.; Verkhoglyadova, Olga; Stephens, Philip; Iijima, Bryron A.

2013-01-01

Modeling and imaging the Earth's ionosphere as well as understanding its structures, inhomogeneities, and disturbances is a key part of NASA's Heliophysics Directorate science roadmap. This invention provides a design tool for scientific missions focused on the ionosphere. It is a scientifically important and technologically challenging task to assess the impact of a new observation system quantitatively on our capability of imaging and modeling the ionosphere. This question is often raised whenever a new satellite system is proposed, a new type of data is emerging, or a new modeling technique is developed. The proposed constellation would be part of a new observation system with more low-Earth orbiters tracking more radio occultation signals broadcast by Global Navigation Satellite System (GNSS) than those offered by the current GPS and COSMIC observation system. A simulation system was developed to fulfill this task. The system is composed of a suite of software that combines the Global Assimilative Ionospheric Model (GAIM) including first-principles and empirical ionospheric models, a multiple- dipole geomagnetic field model, data assimilation modules, observation simulator, visualization software, and orbit design, simulation, and optimization software.

A data delivery system for IMOS, the Australian Integrated Marine Observing System

NASA Astrophysics Data System (ADS)

Proctor, R.; Roberts, K.; Ward, B. J.

2010-09-01

The Integrated Marine Observing System (IMOS, www.imos.org.au), an AUD 150 m 7-year project (2007-2013), is a distributed set of equipment and data-information services which, among many applications, collectively contribute to meeting the needs of marine climate research in Australia. The observing system provides data in the open oceans around Australia out to a few thousand kilometres as well as the coastal oceans through 11 facilities which effectively observe and measure the 4-dimensional ocean variability, and the physical and biological response of coastal and shelf seas around Australia. Through a national science rationale IMOS is organized as five regional nodes (Western Australia - WAIMOS, South Australian - SAIMOS, Tasmania - TASIMOS, New SouthWales - NSWIMOS and Queensland - QIMOS) surrounded by an oceanic node (Blue Water and Climate). Operationally IMOS is organized as 11 facilities (Argo Australia, Ships of Opportunity, Southern Ocean Automated Time Series Observations, Australian National Facility for Ocean Gliders, Autonomous Underwater Vehicle Facility, Australian National Mooring Network, Australian Coastal Ocean Radar Network, Australian Acoustic Tagging and Monitoring System, Facility for Automated Intelligent Monitoring of Marine Systems, eMarine Information Infrastructure and Satellite Remote Sensing) delivering data. IMOS data is freely available to the public. The data, a combination of near real-time and delayed mode, are made available to researchers through the electronic Marine Information Infrastructure (eMII). eMII utilises the Australian Academic Research Network (AARNET) to support a distributed database on OPeNDAP/THREDDS servers hosted by regional computing centres. IMOS instruments are described through the OGC Specification SensorML and where-ever possible data is in CF compliant netCDF format. Metadata, conforming to standard ISO 19115, is automatically harvested from the netCDF files

Earth Observing System. Science and Mission Requirements, Volume 1, Part 1

NASA Technical Reports Server (NTRS)

1984-01-01

The Earth Observing System (EOS) is a planned NASA program, which will carry the multidisciplinary Earth science studies employing a variety of remote sensing techniques in the 1990's, as a prime mission, using the Space Station polar platform. The scientific rationale, recommended observational needs, the broad system configuration and a recommended implementation strategy to achieve the stated mission goals are provided.

State deadbeat response and observability in multi-modal systems

NASA Technical Reports Server (NTRS)

Conner, L. T., Jr.; Stanford, D. P.

1984-01-01

Two aspects of multimodal systems are examined. It is shown that any completely controllable system with state dimension n not exceeding three allows a choice of feedback matrices resulting in a state deadbeat response. Some of the results presented here are valid for arbitrary n, and it is suggested that for all n the state deadbeat response can be obtained under the hypothesis of complete controllability. The controllability canonical form for a multimodal system is refined by introducing a notion of observability which is dual to controllability for these systems.

Development of the AuScope Australian Earth Observing System

NASA Astrophysics Data System (ADS)

Rawling, T.

2017-12-01

Advances in monitoring technology and significant investment in new national research initiatives, will provide significant new opportunities for delivery of novel geoscience data streams from across the Australian continent over the next decade. The AuScope Australian Earth Observing System (AEOS) is linking field and laboratory infrastructure across Australia to form a national sensor array focusing on the Solid Earth. As such AuScope is working with these programs to deploy observational infrastructure, including MT, passive seismic, and GNSS networks across the entire Australian Continent. Where possible the observational grid will be co-located with strategic basement drilling in areas of shallow cover and tied with national reflection seismic and sampling transects. This integrated suite of distributed earth observation and imaging sensors will provide unprecedented imaging fidelity of our crust, across all length and time scales, to fundamental and applied researchers in the earth, environmental and geospatial sciences. The AEOS will the Earth Science community's Square Kilometer Array (SKA) - a distributed telescope that looks INTO the earth rather than away from it - a 10 million SKA. The AEOS is strongly aligned with other community strategic initiatives including the UNCOVER research program as well as other National Collaborative Research Infrastructure programs such as the Terrestrial Environmental Research Network (TERN) and the Integrated Marine Observing System (IMOS) providing an interdisciplinary collaboration platform across the earth and environmental sciences. There is also very close alignment between AuScope and similar international programs such as EPOS, the USArray and EarthCube - potential collaborative linkages we are currently in the process of pursuing more fomally. The AuScope AEOS Infrastructure System is ultimately designed to enable the progressive construction, refinement and ongoing enrichment of a live, "FAIR" four

Assessment of DoD Enterprise Resource Planning Business Systems

DTIC Science & Technology

2011-02-01

activities, and processes to the organizational units that execute them • Architecture standards, such as application of the BPMN • Recommended...Recommendation: • Create and use style guides for the development of BPMN based process models. The style guide would probably include specifications such as...o All processes must have ‘entry points’ or ‘triggers’ in the form of BPMN Events o All processes must have ‘outcomes’ also in the form of BPMN

EXTREME AO OBSERVATIONS OF TWO TRIPLE ASTEROID SYSTEMS WITH SPHERE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, B.; Wahhaj, Z.; Dumas, C.

We present the discovery of a new satellite of asteroid (130) Elektra—S/2014 (130) 1—in differential imaging and in integral field spectroscopy data over multiple epochs obtained with Spectro-Polarimetric High-contrast Exoplanet Research/Very Large Telescope. This new (second) moonlet of Elektra is about 2 km across, on an eccentric orbit, and about 500 km away from the primary. For a comparative study, we also observed another triple asteroid system, (93) Minerva. For both systems, component-resolved reflectance spectra of the satellites and primary were obtained simultaneously. No significant spectral difference was observed between the satellites and the primary for either triple system. Wemore » find that the moonlets in both systems are more likely to have been created by sub-disruptive impacts as opposed to having been captured.« less

Observational studies of the clearing phase in proto-planetary disk systems

NASA Technical Reports Server (NTRS)

Grady, Carol A.

1994-01-01

A summary of the work completed during the first year of a 5 year program to observationally study the clearing phase of proto-planetary disks is presented. Analysis of archival and current IUE data, together with supporting optical observations has resulted in the identification of 6 new proto-planetary disk systems associated with Herbig Ae/Be stars, the evolutionary precursors of the beta Pictoris system. These systems exhibit large amplitude light and optical color variations which enable us to identify additional systems which are viewed through their circumstellar disks including a number of classical T Tauri stars. On-going IUE observations of Herbig Ae/Be and T Tauri stars with this orientation have enabled us to detect bipolar emission plausibly associated with disk winds. Preliminary circumstellar extinction studies were completed for one star, UX Ori. Intercomparison of the available sample of edge-on systems, with stars ranging from 1-6 solar masses, suggests that the signatures of accreting gas, disk winds, and bipolar flows and the prominence of a dust-scattered light contribution to the integrated light of the system decreases with decreasing IR excess.

Global Earth Observation System of Systems (GEOSS): Initial Actions to Enhance Data Sharing to Meet Societal Needs

NASA Astrophysics Data System (ADS)

Adang, T.

2006-05-01

Over 60 nations and 50 participating organizations are working to make the Global Earth Observation System of Systems (GEOSS) a reality. The U.S. contribution to GEOSS is the Integrated Earth Observation System (IEOS), with a vision of enabling a healthy public, economy and planet through an integrated, comprehensive, and sustained Earth observation system. The international Group on Earth Observations (GEO) and the U.S. Group on Earth Observations have developed strategic plans for both GEOSS and IEOS, respectively, and are now working the first phases of implementation. Many of these initial actions are data architecture related and are being addressed by architecture and data working groups from both organizations - the GEO Architecture and Data Committee and the USGEO Architecture and Data Management Working Group. NOAA has actively participated in both architecture groups and has taken internal action to better support GEOSS and IEOS implementation by establishing the Global Earth Observation Integrated Data Environment (GEO IDE). GEO IDE provides a "system of systems" framework for effective and efficient integration of NOAA's many quasi-independent systems, which individually address diverse mandates in such areas resource management, weather forecasting, safe navigation, disaster response, and coastal mapping among others. GEO IDE will have a services oriented architecture, allowing NOAA Line Offices to retain a high level of independence in many of their data management decisions, and encouraging innovation in pursuit of their missions. Through GEO IDE, NOAA partners (both internal and external) will participate in a well-ordered, standards-based data and information infrastructure that will allow users to easily locate, acquire, integrate and utilize NOAA data and information. This paper describes the initial progress being made by GEO and USGEO architecture and data working groups, a status report on GEO IDE development within NOAA, and an assessment of

Ionospheric Simulation System for Satellite Observations and Global Assimilative Modeling Experiments (ISOGAME)

NASA Technical Reports Server (NTRS)

Pi, Xiaoqing; Mannucci, Anthony J.; Verkhoglyadova, Olga P.; Stephens, Philip; Wilson, Brian D.; Akopian, Vardan; Komjathy, Attila; Lijima, Byron A.

2013-01-01

ISOGAME is designed and developed to assess quantitatively the impact of new observation systems on the capability of imaging and modeling the ionosphere. With ISOGAME, one can perform observation system simulation experiments (OSSEs). A typical OSSE using ISOGAME would involve: (1) simulating various ionospheric conditions on global scales; (2) simulating ionospheric measurements made from a constellation of low-Earth-orbiters (LEOs), particularly Global Navigation Satellite System (GNSS) radio occultation data, and from ground-based global GNSS networks; (3) conducting ionospheric data assimilation experiments with the Global Assimilative Ionospheric Model (GAIM); and (4) analyzing modeling results with visualization tools. ISOGAME can provide quantitative assessment of the accuracy of assimilative modeling with the interested observation system. Other observation systems besides those based on GNSS are also possible to analyze. The system is composed of a suite of software that combines the GAIM, including a 4D first-principles ionospheric model and data assimilation modules, an Internal Reference Ionosphere (IRI) model that has been developed by international ionospheric research communities, observation simulator, visualization software, and orbit design, simulation, and optimization software. The core GAIM model used in ISOGAME is based on the GAIM++ code (written in C++) that includes a new high-fidelity geomagnetic field representation (multi-dipole). New visualization tools and analysis algorithms for the OSSEs are now part of ISOGAME.

[Observation of oral actions using digital image processing system].

PubMed

Ichikawa, T; Komoda, J; Horiuchi, M; Ichiba, H; Hada, M; Matsumoto, N

1990-04-01

A new digital image processing system to observe oral actions is proposed. The system provides analyses of motion pictures along with other physiological signals. The major components are a video tape recorder, a digital image processor, a percept scope, a CCD camera, an A/D converter and a personal computer. Five reference points were marked on the lip and eyeglasses of 9 adult subjects. Lip movements were recorded and analyzed using the system when uttering five vowels and [ka, sa, ta, ha, ra, ma, pa, ba[. 1. Positions of the lip when uttering five vowels were clearly classified. 2. Active articulatory movements of the lip were not recognized when uttering consonants [k, s, t, h, r[. It seemed lip movements were dependent on tongue and mandibular movements. Downward and rearward movements of the upper lip, and upward and forward movements of the lower lip were observed when uttering consonants [m, p, b[.

The Coastal Observing System for Northern and Arctic Seas (COSYNA)

NASA Astrophysics Data System (ADS)

Baschek, Burkard; Schroeder, Friedhelm; Brix, Holger; Riethmüller, Rolf; Badewien, Thomas H.; Breitbach, Gisbert; Brügge, Bernd; Colijn, Franciscus; Doerffer, Roland; Eschenbach, Christiane; Friedrich, Jana; Fischer, Philipp; Garthe, Stefan; Horstmann, Jochen; Krasemann, Hajo; Metfies, Katja; Merckelbach, Lucas; Ohle, Nino; Petersen, Wilhelm; Pröfrock, Daniel; Röttgers, Rüdiger; Schlüter, Michael; Schulz, Jan; Schulz-Stellenfleth, Johannes; Stanev, Emil; Staneva, Joanna; Winter, Christian; Wirtz, Kai; Wollschläger, Jochen; Zielinski, Oliver; Ziemer, Friedwart

2017-05-01

The Coastal Observing System for Northern and Arctic Seas (COSYNA) was established in order to better understand the complex interdisciplinary processes of northern seas and the Arctic coasts in a changing environment. Particular focus is given to the German Bight in the North Sea as a prime example of a heavily used coastal area, and Svalbard as an example of an Arctic coast that is under strong pressure due to global change.The COSYNA automated observing and modelling system is designed to monitor real-time conditions and provide short-term forecasts, data, and data products to help assess the impact of anthropogenically induced change. Observations are carried out by combining satellite and radar remote sensing with various in situ platforms. Novel sensors, instruments, and algorithms are developed to further improve the understanding of the interdisciplinary interactions between physics, biogeochemistry, and the ecology of coastal seas. New modelling and data assimilation techniques are used to integrate observations and models in a quasi-operational system providing descriptions and forecasts of key hydrographic variables. Data and data products are publicly available free of charge and in real time. They are used by multiple interest groups in science, agencies, politics, industry, and the public.

Clinical value of antistreptolysin O levels in adult patients with tonsillitis: report I.

PubMed

Trushin, Vladimir; Englender, Moshe

2017-04-01

To assess the clinical value of antistreptolysin O (ASO) level in adult patients with acute tonsillitis of group A beta-hemolytic streptococcus (GABHS) etiology and its interaction with the Centor score and throat cultures data. ASO antibody titers and throat cultures were obtained from 260 adult patients with acute tonsillitis of GABHS etiology initially proven by the Centor score. The results were compared with the group of 100 adult patients with recurrent tonsillitis who underwent tonsillectomy and with the group of 100 healthy adults. Throat cultures revealed GABHS-positive results in 69 acute cases (26.5%) and in 24 recurrent cases (24%), i.e., with no significant differences between the groups (p = 0.845). There was no significant difference between cases with GABHS-positive and with GABHS-negative throat culture in ASO titers results (mean 250 and 280, respectively, p = 0.44) but these titers were significantly higher than established normative data (p < 0.01). For the group of recurrent tonsillitis cases, the mean ASO titer was 363 being significantly higher in comparison with acute cases (p = 0.015). The ASO antibody titers are significantly higher than normative ranges in cases of acute tonsillitis in adults. The detection of the elevated titers may lead to early antibiotherapy to tonsillitis. The Centor score is supported by the ASO data and less supported by throat cultures data. Further research should reveal if these titers might have predictive value for possible further recurrence or serve as an indicator for tonsillectomy in cases of recurrent tonsillitis.

21-cm Observations with the NASA ADAS 18-meter Antenna System: Baseline Astronomical Observations and Measurements of Performance Characteristics

NASA Astrophysics Data System (ADS)

Malphrus, B. K.; Combs, M. S.; Kruth, J.

2001-12-01

Herein we report astronomical observations made with the NASA Advanced Data Acquisition System (ADAS). The NASA ADAS antenna, located at NASA Goddard Spaceflight Center's Wallops Flight Facility, Virginia, is an 18-meter X-band antenna system that has been primarily used for satellite tracking and served as the telecommunication station for the NASA IUE satellite until ca. 1997. A joint NASA-Morehead State University (MSU)-Kentucky NSF EPSCoR venture has been initiated to upgrade and relocate the antenna system to MSU's Astrophysics Laboratory where it will provide a research instrument and active laboratory for undergraduate students as well as be engaged in satellite tracking missions. As part of the relocation efforts, many systems will be upgraded including replacement of a hydrostatic azimuth bearing with a high-precision electromechanical bearing, a new servo system, and Ku-capable reflector surface. It is widely believed that there are still contributions that small aperture centimeter-wave instruments can make utilizing three primary observing strategies: 1.) longitudinal studies of RF variations in cosmic phenomena, 2.) surveys of large areas of sky, and 3.) fast reactions to transient phenomena. MSU faculty and staff along with NASA engineers re-outfitted the ADAS system with RF systems and upgraded servo controllers during the spring and summer of 2001. Empirical measurements of primary system performance characteristics were made including G/T (at S- and L bands), noise figures, pointing and tracking accuracies, and drive speeds and accelerations. Baseline astronomical observations were made with the MSU L-band receiver using a 6 MHz bandwidth centered at 1420 MHz (21-cm) and observing over a range of frequencies (up to 2.5 MHz, tunable over the 6 MHz window) with a 2048-channel back-end spectrometer, providing up to 1 KHz frequency resolution. Baseline observations of radio sources herein reported include Cygnus A, 3C 157, 3C 48 and the Andromeda

Toward an integrated Volcanic Ash Observing System in Europe

NASA Astrophysics Data System (ADS)

Lee, Deborah; Lisk, Ian

2014-05-01

Volcanic ash from the Icelandic eruption of Eyjafjallajökull in April and May of 2010 resulted in the decision by many northern European countries to impose significant restrictions on the use of their airspace. The eruption, extent and persistence of the ash revealed how reliant society now is on a safe and efficient air transport system and the fragility of that system when affected by the impact of complex natural hazards. As part of an EC framework programme, the 2011-2013 WEZARD (WEather HaZARD for aeronautics) consortium conducted a cross-industry volcanic ash capability and gap analyses, with the EUMETNET (network of 29 National Meteorological Services) led Work Package 3 focussing on a review of observational and monitoring capabilities, atmospheric dispersion modelling and data exchange. The review has revealed a patchwork of independent observing capabilities for volcanic ash, with some countries investing and others not at all, and most existing networks focus on space-based products. Existing capabilities do not provide the necessary detail on the geographical and vertical extent of volcanic ash and associated levels of contamination, which decision makers in the aviation industry require in order to decide where it is safe to fly. A resultant high priority was identified by WEZARD Work Package 3 for an enhanced observational network of complementary monitoring systems needed to initialise, validate and verify volcanic ash dispersion model output and forecasts. Thus a key recommendation is to invest in a major pre-operational demonstrator "European volcanic ash observing network", focussing on distal monitoring, and aiming to a) fill R&D gaps identified in instrumentation and algorithms and b) integrate data, where possible in near-real-time, from a range of ground-based, airborne and space-based techniques. Here we present a key WEZARD recommendation toward an integrated volcanic ash observing system in Europe, in context with other related projects

Radiometric calibration of the Earth observing system's imaging sensors

NASA Technical Reports Server (NTRS)

Slater, P. N.

1987-01-01

Philosophy, requirements, and methods of calibration of multispectral space sensor systems as applicable to the Earth Observing System (EOS) are discussed. Vicarious methods for calibration of low spatial resolution systems, with respect to the Advanced Very High Resolution Radiometer (AVHRR), are then summarized. Finally, a theoretical introduction is given to a new vicarious method of calibration using the ratio of diffuse-to-global irradiance at the Earth's surfaces as the key input. This may provide an additional independent method for in-flight calibration.

The Impact of British Airways Wind Observations on the Goddard Earth Observing System Analyses and Forecasts

NASA Technical Reports Server (NTRS)

Rukhovets, Leonid; Sienkiewicz, M.; Tenenbaum, J.; Kondratyeva, Y.; Owens, T.; Oztunali, M.; Atlas, Robert (Technical Monitor)

2001-01-01

British Airways flight data recorders can provide valuable meteorological information, but they are not available in real-time on the Global Telecommunication System. Information from the flight recorders was used in the Global Aircraft Data Set (GADS) experiment as independent observations to estimate errors in wind analyses produced by major operational centers. The GADS impact on the Goddard Earth Observing System Data Assimilation System (GEOS DAS) analyses was investigated using GEOS-1 DAS version. Recently, a new Data Assimilation System (fvDAS) has been developed at the Data Assimilation Office, NASA Goddard. Using fvDAS , the, GADS impact on analyses and forecasts was investigated. It was shown the GADS data intensify wind speed analyses of jet streams for some cases. Five-day forecast anomaly correlations and root mean squares were calculated for 300, 500 hPa and SLP for six different areas: Northern and Southern Hemispheres, North America, Europe, Asia, USA These scores were obtained as averages over 21 forecasts from January 1998. Comparisons with scores for control experiments without GADS showed a positive impact of the GADS data on forecasts beyond 2-3 days for all levels at the most areas.

Solar System Observing with the Space Infrared Telescope Facility (SIRTF)

NASA Technical Reports Server (NTRS)

Cleve, J. Van; Meadows, V. S.; Stansberry, J.

2003-01-01

SIRTF is NASA's Space Infrared Telescope Facility. Currently planned for launch on 15 Apr 2003, it is the final element in NASA's Great Observatories Program. SIRTF has an 85 cm diameter f/12 lightweight beryllium telescope, cooled to lekss than 5.5K. It is diffraction-limited at 6.5 microns, and has wavelengthcoverage from 3-180 microns. Its estimated lifetime (limited by cryogen) is 2.5 years at minimum, with a goal of 5+ years. SIRTF has three instruments, IRAC, IRS, and MIPS. IRAC (InfraRed Array Camera) provides simultaneous images at wavelengths of 3.6, 4.5, 5.8, and 8.0 microns. IRS (InfraRed Spectrograph) has 4 modules providing low-resolution (R=60-120) spectra from 5.3 to 40 microns, high-resolution (R=600) spectra from 10 to 37 microns, and an autonomous target acquisition system (PeakUp) which includes small-field imaging at 15 microns. MIPS (Multiband Imaging Photometer for SIRTF)} does imaging photometry at 24, 70, and 160 m and low-resolution (R=15-25) spectroscopy (SED) between 55 and 96 microns. The SIRTF Guaranteed Time Observers (GTOs) are planning to observe Outer Solar System satellites and planets, extinct comets and low-albedo asteroids, Centaurs and Kuiper Belt Objects, cometary dust trails, and a few active short-period comets. The GTO programs are listed in detail in the SIRTF Reserved Observations Catalog (ROC). We would like to emphasize that there remain many interesting subjects for the General Observers (GO). Proposal success for the planetary observer community in the first SIRTF GO proposal cycle (GO-1) determines expectations for future GO calls and Solar System use of SIRTF, so we would like promote a strong set of planetary GO-1 proposals. Towards that end, we present this poster, and we will convene a Solar System GO workshop 3.5 months after launch.

OOSTethys - Open Source Software for the Global Earth Observing Systems of Systems

NASA Astrophysics Data System (ADS)

Bridger, E.; Bermudez, L. E.; Maskey, M.; Rueda, C.; Babin, B. L.; Blair, R.

2009-12-01

An open source software project is much more than just picking the right license, hosting modular code and providing effective documentation. Success in advancing in an open collaborative way requires that the process match the expected code functionality to the developer's personal expertise and organizational needs as well as having an enthusiastic and responsive core lead group. We will present the lessons learned fromOOSTethys , which is a community of software developers and marine scientists who develop open source tools, in multiple languages, to integrate ocean observing systems into an Integrated Ocean Observing System (IOOS). OOSTethys' goal is to dramatically reduce the time it takes to install, adopt and update standards-compliant web services. OOSTethys has developed servers, clients and a registry. Open source PERL, PYTHON, JAVA and ASP tool kits and reference implementations are helping the marine community publish near real-time observation data in interoperable standard formats. In some cases publishing an OpenGeospatial Consortium (OGC), Sensor Observation Service (SOS) from NetCDF files or a database or even CSV text files could take only minutes depending on the skills of the developer. OOSTethys is also developing an OGC standard registry, Catalog Service for Web (CSW). This open source CSW registry was implemented to easily register and discover SOSs using ISO 19139 service metadata. A web interface layer over the CSW registry simplifies the registration process by harvesting metadata describing the observations and sensors from the “GetCapabilities” response of SOS. OPENIOOS is the web client, developed in PERL to visualize the sensors in the SOS services. While the number of OOSTethys software developers is small, currently about 10 around the world, the number of OOSTethys toolkit implementers is larger and growing and the ease of use has played a large role in spreading the use of interoperable standards compliant web services widely

Sorption of As(V) on aluminosilicates treated with Fe(II) nanoparticles.

PubMed

Dousová, Barbora; Grygar, Tomás; Martaus, Alexandr; Fuitová, Lucie; Kolousek, David; Machovic, Vladimír

2006-10-15

Adsorption of arsenic on clay surfaces is important for the natural and simulated removal of arsenic species from aqueous environments. In this investigation, three samples of clay minerals (natural metakaoline, natural clinoptilolite-rich tuff, and synthetic zeolite) in both untreated and Fe-treated forms were used for the sorption of arsenate from model aqueous solution. The treatment of minerals consisted of exposing them to concentrated solution of Fe(II). Within this process the mineral surface has been laden with Fe(III) oxi(hydroxides) whose high affinity for the As(V) adsorption is well known. In all investigated systems the sorption capacity of Fe(II)-treated sorbents increased significantly in comparison to the untreated material (from about 0.5 to >20.0 mg/g, which represented more than 95% of the total As removal). The changes of Fe-bearing particles in the course of treating process and subsequent As sorption were investigated by the diffuse reflectance spectroscopy and the voltammetry of microparticles. IR spectra of treated and As(V)-saturated solids showed characteristic bands caused by Fe(III)SO(4), Fe(III)O, and AsO vibrations. In untreated As(V)-saturated solids no significant AsO vibrations were observed due to the negligible content of sorbed arsenate.

Climate Outreach Using Regional Coastal Ocean Observing System Portals

NASA Astrophysics Data System (ADS)

Anderson, D. M.; Hernandez, D. L.; Wakely, A.; Bochenek, R. J.; Bickel, A.

2015-12-01

Coastal oceans are dynamic, changing environments affected by processes ranging from seconds to millennia. On the east and west coast of the U.S., regional observing systems have deployed and sustained a remarkable diverse array of observing tools and sensors. Data portals visualize and provide access to real-time sensor networks. Portals have emerged as an interactive tool for educators to help students explore and understand climate. Bringing data portals to outreach events, into classrooms, and onto tablets and smartphones enables educators to address topics and phenomena happening right now. For example at the 2015 Charleston Science Technology Engineering and Math (STEM) Festival, visitors navigated the SECOORA (Southeast Coastal Ocean Observing regional Association) data portal to view the real-time marine meteorological conditions off South Carolina. Map-based entry points provide an intuitive interface for most students, an array of time series and other visualizations depict many of the essential principles of climate science manifest in the coastal zone, and data down-load/ extract options provide access to the data and documentation for further inquiry by advanced users. Beyond the exposition of climate principles, the portal experience reveals remarkable technologies in action and shows how the observing system is enabled by the activity of many different partners.

Dmpk gene deletion or antisense knockdown does not compromise cardiac or skeletal muscle function in mice

PubMed Central

Carrell, Samuel T.; Carrell, Ellie M.; Auerbach, David; Pandey, Sanjay K.; Bennett, C. Frank; Dirksen, Robert T.; Thornton, Charles A.

2016-01-01

Myotonic dystrophy type 1 (DM1) is a genetic disorder in which dominant-active DM protein kinase (DMPK) transcripts accumulate in nuclear foci, leading to abnormal regulation of RNA processing. A leading approach to treat DM1 uses DMPK-targeting antisense oligonucleotides (ASOs) to reduce levels of toxic RNA. However, basal levels of DMPK protein are reduced by half in DM1 patients. This raises concern that intolerance for further DMPK loss may limit ASO therapy, especially since mice with Dmpk gene deletion reportedly show cardiac defects and skeletal myopathy. We re-examined cardiac and muscle function in mice with Dmpk gene deletion, and studied post-maturity knockdown using Dmpk-targeting ASOs in mice with heterozygous deletion. Contrary to previous reports, we found no effect of Dmpk gene deletion on cardiac or muscle function, when studied on two genetic backgrounds. In heterozygous knockouts, the administration of ASOs reduced Dmpk expression in cardiac and skeletal muscle by > 90%, yet survival, electrocardiogram intervals, cardiac ejection fraction and muscle strength remained normal. The imposition of cardiac stress by pressure overload, or muscle stress by myotonia, did not unmask a requirement for DMPK. Our results support the feasibility and safety of using ASOs for post-transcriptional silencing of DMPK in muscle and heart. PMID:27522499

Comparative analysis of minimal residual disease detection using four-color flow cytometry, consensus IgH-PCR, and quantitative IgH PCR in CLL after allogeneic and autologous stem cell transplantation.

PubMed

Böttcher, S; Ritgen, M; Pott, C; Brüggemann, M; Raff, T; Stilgenbauer, S; Döhner, H; Dreger, P; Kneba, M

2004-10-01

The clinically most suitable method for minimal residual disease (MRD) detection in chronic lymphocytic leukemia is still controversial. We prospectively compared MRD assessment in 158 blood samples of 74 patients with CLL after stem cell transplantation (SCT) using four-color flow cytometry (MRD flow) in parallel with consensus IgH-PCR and ASO IgH real-time PCR (ASO IgH RQ-PCR). In 25 out of 106 samples (23.6%) with a polyclonal consensus IgH-PCR pattern, MRD flow still detected CLL cells, proving higher sensitivity of flow cytometry over PCR-genescanning with consensus IgH-primers. Of 92 samples, 14 (15.2%) analyzed in parallel by MRD flow and by ASO IgH RQ-PCR were negative by our flow cytometric assay but positive by PCR, thus demonstrating superior sensitivity of RQ-PCR with ASO primers. Quantitative MRD levels measured by both methods correlated well (r=0.93). MRD detection by flow and ASO IgH RQ-PCR were equally suitable to monitor MRD kinetics after allogeneic SCT, but the PCR method detected impending relapses after autologous SCT earlier. An analysis of factors that influence sensitivity and specificity of flow cytometry for MRD detection allowed to devise further improvements of this technique.

RBS characterization of arsenic(III) partitioning from aqueous phase into the active layers of thin-film composite NF/RO membranes.

PubMed

Mi, Baoxia; Mariñas, Benito J; Cahill, David G

2007-05-01

The main objective of this study was to apply Rutherford backscattering spectrometry (RBS) for characterizing the partitioning of arsenic(III) from aqueous phase into the active layer of NF/RO membranes. NF/RO membranes with active layer materials including polyamide (PA), PA-polyvinyl alcohol derivative (PVA), and sulfonated-polyethersulfone (SPES) were investigated. The partition coefficient was found to be constant in the investigated As-(III) concentration range of 0.005-0.02 M at each pH investigated. The partitioning of As(III) when predominantly present as H3AsO3 (pH 3.5-8.0) was not affected by pH. In contrast, the partition coefficient of As(III) at pH 10.5, when it was predominantly present as H2AsO3-, was found to be approximately 33-49% lower than that of H3AsO3. The partition coefficients of H3AsO3 and H2AsO3- for membranes containing PA in their active layers were within the respective ranges of 6.2-8.1 and 3.6-5.4, while the corresponding values (4.8 and 3.0, respectively) for the membrane with SPES active layer were approximately 30% lower than the average values for the PA membranes.

Liver-specific inhibition of acyl-coenzyme a:cholesterol acyltransferase 2 with antisense oligonucleotides limits atherosclerosis development in apolipoprotein B100-only low-density lipoprotein receptor-/- mice.

PubMed

Bell, Thomas A; Brown, J Mark; Graham, Mark J; Lemonidis, Kristina M; Crooke, Rosanne M; Rudel, Lawrence L

2006-08-01

The purpose of this study was to determine the effects of liver-specific inhibition of acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) on the development of hypercholesterolemia and atherosclerosis in mice. Apolipoprotein B100-only low-density lipoprotein (LDL) receptor-/- mice were given saline, a nontargeting control antisense oligonucleotide (ASO), or ASOs targeting ACAT2 biweekly for a period spanning 16 weeks. Mice treated with ACAT2 targeting ASOs had liver-specific reduction in ACAT2 mRNA, yet intestinal ACAT2 and cholesterol absorption was left undisturbed. ASO-mediated knockdown of ACAT2 resulted in reduction of total plasma cholesterol, increased levels of plasma triglyceride, and a shift in LDL cholesteryl ester (CE) fatty acid composition from mainly saturated and monounsaturated to polyunsaturated fatty acid enrichment. Furthermore, the liver-specific depletion of ACAT2 resulted in protection against diet-induced hypercholesterolemia and aortic CE deposition. This is the first demonstration that specific pharmacological inhibition of ACAT2, without affecting ACAT1, is atheroprotective. Hepatic ACAT2 plays a critical role in driving the production of atherogenic lipoproteins, and therapeutic interventions, such as the ACAT2-specific ASOs used here, which reduce acyltransferase 2 (ACAT2) function in the liver without affecting ACAT1, may provide clinical benefit for cardiovascular disease prevention.

Reduction of VLDL secretion decreases cholesterol excretion in niemann-pick C1-like 1 hepatic transgenic mice.

PubMed

Marshall, Stephanie M; Kelley, Kathryn L; Davis, Matthew A; Wilson, Martha D; McDaniel, Allison L; Lee, Richard G; Crooke, Rosanne M; Graham, Mark J; Rudel, Lawrence L; Brown, J Mark; Temel, Ryan E

2014-01-01

An effective way to reduce LDL cholesterol, the primary risk factor of atherosclerotic cardiovascular disease, is to increase cholesterol excretion from the body. Our group and others have recently found that cholesterol excretion can be facilitated by both hepatobiliary and transintestinal pathways. However, the lipoprotein that moves cholesterol through the plasma to the small intestine for transintestinal cholesterol efflux (TICE) is unknown. To test the hypothesis that hepatic very low-density lipoproteins (VLDL) support TICE, antisense oligonucleotides (ASO) were used to knockdown hepatic expression of microsomal triglyceride transfer protein (MTP), which is necessary for VLDL assembly. While maintained on a high cholesterol diet, Niemann-Pick C1-like 1 hepatic transgenic (L1Tg) mice, which predominantly excrete cholesterol via TICE, and wild type (WT) littermates were treated with control ASO or MTP ASO. In both WT and L1Tg mice, MTP ASO decreased VLDL triglyceride (TG) and cholesterol secretion. Regardless of treatment, L1Tg mice had reduced biliary cholesterol compared to WT mice. However, only L1Tg mice treated with MTP ASO had reduced fecal cholesterol excretion. Based upon these findings, we conclude that VLDL or a byproduct such as LDL can move cholesterol from the liver to the small intestine for TICE.

Generalised observer design for dissipative Lipschitz nonlinear systems in the presence of measurement noise

NASA Astrophysics Data System (ADS)

Vijayaraghavan, Krishna

2014-11-01

This paper presents two novel observer concepts. First, it develops a globally exponentially stable nonlinear observer for noise-free dissipative nonlinear systems. Second, for a dissipative nonlinear system with measurement noise, the paper develops an observer to guarantee a desired performance, namely an upper limit on the ratio of the square of the weighted L2 norm of the error to the square of the weighted L2 norm of the measurement noise. The necessary and sufficient conditions for both observers are reformulated as algebraic Riccati equations (AREs) so that standard solvers can be utilised. In addition, the paper presents necessary and sufficient conditions to be satisfied by the nonlinear system in order to ensure that the ARE (and hence the observer design problem) has a solution. The use of the methodology developed in this paper is demonstrated through illustrative examples. In literature, there is no previous observer for dissipative system that provides both necessary and sufficient conditions. Results for noisy system either rely on linearising the system about state trajectory (requiring initial estimates to be close to the actual states) or are for specialised systems that cannot be extended to dissipative systems.

An operational, multistate, earth observation data management system

NASA Technical Reports Server (NTRS)

Eastwood, L. F., Jr.; Hays, T. R.; Hill, C. T.; Ballard, R. J.; Morgan, R. P.; Crnkovich, G. G.; Gohagan, J. K.; Schaeffer, M. A.

1977-01-01

The purpose of this paper is to investigate a group of potential users of satellite remotely sensed data - state, local, and regional agencies involved in natural resources management. We assess this group's needs in five states and outline alternative data management systems to serve some of those needs. We conclude that an operational Earth Observation Data Management System (EODMS) will be of most use to these user agencies if it provides a full range of information services - from raw data acquisition to interpretation and dissemination of final information products.

Further development of the EUMETNET Composite Observing System (EUCOS)

NASA Astrophysics Data System (ADS)

Klink, S.; Dibbern, J.

2009-09-01

EUCOS, which stands for EUMETNET Composite Observing System, is a EUMETNET programme whose main objective is a central management of surface based operational observations on a European-wide scale serving the needs of regional scale NWP. EUMETNET is a consortium of currently 26 national meteorological services in Europe that provides a framework for different operational and developmental co-operative programmes between the services. The work content of the EUCOS Programme includes the management of the operational observing networks, through the E-AMDAR, E-ASAP, E-SURFMAR and E-WINPROF programmes. The coordination of NMSs owned territorial networks (e.g. radiosonde stations and synoptic stations), data quality monitoring, fault reporting and recovery, a studies programme for the evolution of the observing networks and liaison with other organisations like WMO are among the tasks of the programme. The current period of the EUCOS programme has a five year duration (2007-2011) and a two stage approach was proposed in the programme definition. During the transition phase 2007-2008 no new programmatic objectives had been set because amongst others the Space-Terrestrial (S-T) study which investigated the relative contributions of selected space based and ground based observing systems to the forecast skill of global and regional NWP models had to be finalised first. Based on the findings of this study EUCOS currently prepares a redesign of its upper-air network. The original EUCOS upper-air network design was prepared in 2000 in order to define a set of stations serving the common general NWP requirement. Additional considerations were to make it possible to supply a common set of performance standards across the territory of EUMETNET Members and to ensure that the radiosonde network interleaved with AMDAR airports. The EUCOS upper-air network now requires a redesign because of several reasons. There is a need to take into account the significant evolution of the AMDAR

Development of a System for Observing Dance Activities in the Classroom Environment (SODANCE)

ERIC Educational Resources Information Center

Sims, Meredith; Abel, Mark; Clasey, Jody; Beighle, Aaron; Fedewa, Alicia; Erwin, Heather

2016-01-01

This study sought to develop a system for observing dance activities in the classroom environment (SODANCE) based on the system for observing fitness instructional time (SOFIT). SODANCE utilizes direct observation to quantify the physical activity intensity of dance activities. Female students aged 11-17 years participated in an activity protocol…

Operative planning of functional sessions for multisatellite observation and communication systems

NASA Astrophysics Data System (ADS)

Darnopykh, Valeriy V.; Malyshev, Veniamin V.

2012-04-01

An important control aspect of modern satellite observation and communication systems is the control of the functional processes. Functional sessions proceed under conditions of restricted technical ability, large amounts or information to be processed by the on-board equipment, practice inequality of the received information, intentions of system management and operators, interests of customers and other factors. A large number of spacecrafts (SC) in orbital constellation is one of the most important factors affecting the functional process also. Besides that some modern projects of satellite systems are multifunctional that is mixed operations of observation and communication. Therefore the functioning of SC on-board equipment must be accurately co-ordinate. That is why the problem of operative planning the functioning of these systems, while directly affecting the efficiency of the system, is very complex and actual at present. A methodical approach and software package for operative planning of functional processes for satellite observation and communication systems, including multifunctional projects, are considered in the paper. The base scheme of this approach consists of four main stages: stage 1—modeling of SC orbital kinematics and dynamics; stage 2—modeling of system functional processes with all kind of restrictions and criterion function values; stage 3—solving an optimization tasks by numerical applicable algorithms and constructing the optimal (or accuracy) plans; stage 4—repeated plan optimization (different variants) and analyzing. Such scheme is the result of authors practical research which have been realized during last 15 years by the operative planning as for any kinds of single SC as for satellite systems with different structure of orbital constellation. The research helps to unify the procedure of operative planning, to formulate basic principles and approaches for its solving, to develop special software package. The main aspects