Fundamental studies of MALDI with an orthogonal TOF mass spectrometer
NASA Astrophysics Data System (ADS)
Qiao, Hui
The interaction between the matrix and analyte molecules are studied with a high resolution MALDI imaging technique in an orthogonal-injection time of flight (TOF) mass spectrometer. The analyte incorporation and distribution patterns have been clearly demonstrated. Purified protein analytes were found to be homogeneously incorporated in large single crystals of DHB and sinapinic acid matrices, with no evidence for preferred crystal faces. Segregation of some species was observed and appeared to correlate with analyte hydrophobicity, and to a lesser extent analyte mass or mobility. Similar segregation phenomena were observed with confocal laser scanning microscopy of the same analytes labeled with fluorescent dyes in 2,5-DHB single crystals. The above investigations may shed some light on optimizing sample preparation with different matrices. The influence of incident laser parameters on sensitivity in MALDI has been investigated using orthogonal-injection TOF instruments. A qualitative comparison was first made between the beam profiles obtained with a N 2 laser and a Nd:YAG laser using 2-m long optical fibers. The N 2 laser gives better sensitivity, consistent with a more uniform fluence distribution and therefore better coverage of the N2 laser profile. Most of the difference disappears when a 30-m long fiber is used or when the fibers are twisted during irradiation to smooth out the fluence distribution. In more systematic measurements, the total integrated ion yield from a single spot (a measure of sensitivity) was found to increase rapidly with fluence to a maximum, and then saturate or decrease slightly. Thus, the optimum sensitivity is achieved at high fluence. For a fluence near threshold, the integrated yield has a steep (cubic) dependence on the spot size, but the yield saturates at higher fluence for smaller spots. The area dependence is much weaker (close to linear) for fluence values above saturation, with the result that the highest integrated yields
Orthogonal ion injection apparatus and process
Kurulugama, Ruwan T; Belov, Mikhail E
2014-04-15
An orthogonal ion injection apparatus and process are described in which ions are directly injected into an ion guide orthogonal to the ion guide axis through an inlet opening located on a side of the ion guide. The end of the heated capillary is placed inside the ion guide such that the ions are directly injected into DC and RF fields inside the ion guide, which efficiently confines ions inside the ion guide. Liquid droplets created by the ionization source that are carried through the capillary into the ion guide are removed from the ion guide by a strong directional gas flow through an inlet opening on the opposite side of the ion guide. Strong DC and RF fields divert ions into the ion guide. In-guide orthogonal injection yields a noise level that is a factor of 1.5 to 2 lower than conventional inline injection known in the art. Signal intensities for low m/z ions are greater compared to convention inline injection under the same processing conditions.
MALDI-TOF and nESI Orbitrap MS/MS identify orthogonal parts of the phosphoproteome.
Ruprecht, Benjamin; Roesli, Christoph; Lemeer, Simone; Kuster, Bernhard
2016-05-01
Phosphorylation is a reversible posttranslational protein modification which plays a pivotal role in intracellular signaling. Despite extensive efforts, phosphorylation site mapping of proteomes is still incomplete motivating the exploration of alternative methods that complement existing workflows. In this study, we compared tandem mass spectrometry (MS/MS) on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) and nano-electrospray ionization (nESI) Orbitrap instruments with respect to their ability to identify phosphopeptides from complex proteome digests. Phosphopeptides were enriched from tryptic digests of cell lines using Fe-IMAC column chromatography and subjected to LC-MS/MS analysis. We found that the two analytical workflows exhibited considerable orthogonality. For instance, MALDI-TOF MS/MS favored the identification of phosphopeptides encompassing clear motif signatures for acidic residue directed kinases. The extent of orthogonality of the two LC-MS/MS systems was comparable to that of using alternative proteases such as Asp-N, Arg-C, chymotrypsin, Glu-C and Lys-C on just one LC-MS/MS instrument. Notably, MALDI-TOF MS/MS identified an unexpectedly high number and percentage of phosphotyrosine sites (∼20% of all sites), possibly as a direct consequence of more efficient ionization. The data clearly show that LC-MALDI MS/MS can be a useful complement to LC-nESI MS/MS for phosphoproteome mapping and particularly so for acidic and phosphotyrosine containing peptides. PMID:26990019
Gatare, I.; Panajotov, K.; Sciamanna, M.
2007-02-15
We report theoretically on a pure frequency-induced polarization bistability in a vertical-cavity surface-emitting laser (VCSEL) subject to orthogonal optical injection, i.e., the master laser light polarization is orthogonal to that of the slave VCSEL. As the frequency detuning is scanned from negative to positive values and for a fixed injected power, the VCSEL exhibits two successive and possibly bistable polarization switchings. The first switching (from the slave laser polarization to the injected light polarization) exhibits a bistable region whose width is maximum for a given value of the injected power. Such a dependency of hysteresis width on the injected power is similar to that recently found experimentally by Hong et al.[Electron. Lett. 36, 2019 (2000)]. The bistability accompanying the second switching (from the injected light polarization back to the slave laser free-running polarization) exhibits, however, significantly different features related to the occurrence of optical chaos. Interestingly, the width of the bistable region can be tuned over a large range not only by modifying the injection parameters but also by modifying the device parameters, in particular the VCSEL linewidth enhancement factor.
Li, Sensen; Lin, Zongtao; Jiang, Haixiu; Tong, Lingkun; Wang, Hong; Chen, Shizhong
2016-08-01
Fufang Banbianlian Injection (FBI) is a well-known traditional Chinese medicine formula composed of three herbal medicines. However, the systematic investigation on its chemical components has not been reported yet. In this study, a high-performance liquid chromatography combined with diode-array detector, and coupled to an electrospray ionization with ion-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method, was established for the identification of chemical profile in FBI. Sixty-six major constituents (14 phenolic acids, 14 iridoids, 20 flavonoids, 2 benzylideneacetone compounds, 3 phenylethanoid glycosides, 1 coumarin, 1 lignan, 3 nucleosides, 1 amino acids, 1 monosaccharides, 2 oligosaccharides, 3 alduronic acids and citric acid) were identified or tentatively characterized by comparing their retention times and MS spectra with those of standards or literature data. Finally, all constituents were further assigned in the individual herbs (InHs), although some of them were from multiple InHs. As a result, 11 compounds were from Lobelia chinensis Lour, 33 compounds were from Scutellaria barbata D. Don and 38 compounds were from Hedyotis diffusa Willd. In conclusion, the developed HPLC-DAD-ESI-IT-TOF-MS method is a rapid and efficient technique for analysis of FBI sample, and could be a valuable method for the further study on the quality control of the FBI. PMID:27107094
Chen, Tsung-Chi; Fillmore, Thomas L.; Prost, Spencer A.; Moore, Ronald J.; Ibrahim, Yehia M.; Smith, Richard D.
2015-06-24
The electrodynamic ion funnel facilitates efficient focusing and transfer of charged particles in the higher pressure regions (e.g. ion source interfaces) of mass spectrometers, and thus providing increased sensitivity. An “off-axis” ion funnel design has been developed to reduce the source contamination and interferences from, e.g. ESI droplet residue and other poorly focused neutral or charged particles with very high mass-to charge ratios. In this study a dual ion funnel interface consisting of an orthogonal higher pressure electrodynamic ion funnel (HPIF) and an ion funnel trap combined with a triple quadruple mass spectrometer was developed and characterized. An orthogonal ion injection inlet and a repeller plate electrode was used to direct ions to an ion funnel HPIF at 9-10 Torr pressure. Several critical factors for the HPIF were characterized, including the effects of RF amplitude, DC gradient and operating pressure. Compared to the triple quadrupole standard interface more than 4-fold improvement in the limit of detection for the direct quantitative MS analysis of low abundance peptides was observed. Lastly, the sensitivity enhancement in liquid chromatography selected reaction monitoring (SRM) analyses of low abundance peptides spiked into a highly complex mixture was also compared with that obtained using a both commercial s-lens interface and a in-line dual ion funnel interface.
Chen, Tsung-Chi; Fillmore, Thomas L.; Prost, Spencer A.; Moore, Ronald J.; Ibrahim, Yehia M.; Smith, Richard D.
2015-06-24
The electrodynamic ion funnel facilitates efficient focusing and transfer of charged particles in the higher pressure regions (e.g. ion source interfaces) of mass spectrometers, and thus providing increased sensitivity. An “off-axis” ion funnel design has been developed to reduce the source contamination and interferences from, e.g. ESI droplet residue and other poorly focused neutral or charged particles with very high mass-to charge ratios. In this study a dual ion funnel interface consisting of an orthogonal higher pressure electrodynamic ion funnel (HPIF) and an ion funnel trap combined with a triple quadruple mass spectrometer was developed and characterized. An orthogonal ionmore » injection inlet and a repeller plate electrode was used to direct ions to an ion funnel HPIF at 9-10 Torr pressure. Several critical factors for the HPIF were characterized, including the effects of RF amplitude, DC gradient and operating pressure. Compared to the triple quadrupole standard interface more than 4-fold improvement in the limit of detection for the direct quantitative MS analysis of low abundance peptides was observed. Lastly, the sensitivity enhancement in liquid chromatography selected reaction monitoring (SRM) analyses of low abundance peptides spiked into a highly complex mixture was also compared with that obtained using a both commercial s-lens interface and a in-line dual ion funnel interface.« less
Chen, Tsung-Chi; Fillmore, Thomas L.; Prost, Spencer A.; Moore, Ronald J.; Ibrahim, Yehia M.; Smith, Richard D.
2015-07-21
The electrodynamic ion funnel facilitates efficient focusing and transfer of charged particles in the higher pressure regions (e.g. ion source interfaces) of mass spectrometers, and thus providing increased sensitivity. An “off-axis” ion funnel design has been developed to reduce the source contamination and interferences from, e.g. ESI droplet residue and other poorly focused neutral or charged particles with very high mass-to charge ratios. In this study a dual ion funnel interface consisting of an orthogonal higher pressure electrodynamic ion funnel (HPIF) and an ion funnel trap combined with a triple quadruple mass spectrometer was developed and characterized. An orthogonal ion injection inlet and a repeller plate electrode was used to direct ions to an ion funnel HPIF at 9-10 Torr pressure. Several critical factors for the HPIF were characterized, including the effects of RF amplitude, DC gradient and operating pressure. Compared to the triple quadrupole standard interface more than 4-fold improvement in the limit of detection for the direct quantitative MS analysis of low abundance peptides was observed. The sensitivity enhancement in liquid chromatography selected reaction monitoring (SRM) analyses of low abundance peptides spiked into a highly complex mixture was also compared with that obtained using a both commercial s-lens interface and a in-line dual ion funnel interface.
Yang, Yidi; Zhu, Xi; Zhang, Fei; Li, Wei; Wu, Ying; Ding, Li
2016-06-01
Stress testing was carried out under acidic, alkaline, oxidative, thermal and photolytic conditions to evaluate the intrinsic stability of posaconazole injection. A total of four degradation products were detected and the drug was found to be susceptible to oxidative and thermal degradations. Three unknown degradants formed under oxidative stress condition were isolated by preparative HPLC and unambiguously elucidated by LC-TOF/MS, LC-MS/MS, (1)H NMR, (13)C NMR and 2D NMR techniques. Based on the spectrometric and spectroscopic information, these novel degradation products were unequivocally assigned as the N-oxides of posaconazole. Probable mechanisms for the formation of the degradants were proposed. A new and selective HPLC method was developed and validated to separate, detect and quantify all the degradants in posaconazole injection. PMID:27023129
Zhang, Shan; Li, Yan-Jing; Zhang, Chun-Xiao; Huang, Wen-Zhe; Ding, Gang; Wang, Zhen-Zhong; Bi, Yu-An; Xiao, Wei
2016-02-01
A high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) was developed for the analysis of chemical composition change in the production process of Re Du Ning injection, a Chinese medicine preparation with a combination of Lonicera japonica Thunb., Gardenia jasminoides Ellis and Artemisia annua L. A total of 90 compounds from raw materials-intermediates-Re Du Ning injection were detected; among them, 55 compounds were identified or tentatively characterized, and the characteristic ions of different types of compounds were described. Based on these studies, the different types of compounds in the various process routes were analyzed. A total of 28 compounds, including seven iridoid glycosides and six monoterpenes from G. jasminoides Ellis, five iridoid glycosides, nine phenolic acids and one unknown compound from L. japonica Thunb., were transferred to Re Du Ning injection, and two unknown compounds were generated in the production process of Re Du Ning injection. The results indicated that the Chinese Medicine Pharmaceutical process control is very important. This method could provide some reference for other Chinese medicine preparations. PMID:26058547
Sun, Hongyang; Liu, Meixian; Lin, Zongtao; Jiang, Haixiu; Niu, Yanyan; Wang, Hong; Chen, Shizhong
2015-11-10
A high-performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method was established for excellent separation and structural identification of constituents in Shuang-huang-lian powder injection (SHLPI). The typical ultraviolet absorptions, accurate empirical molecular formula and reasonable fragmentation mechanisms of these ingredients were used for their structural elucidation. In consequence, 125 constituents (33 phenolic acids, 29 flavonoids, 32 phenylethanoid glycosides, 15 iridoid glycosides, 8 lignans, 3 amino acids and 2 purines nucleosides, 2 quinoid glycosides and 1 alkylbenzene glycoside) were either unequivocally identified or tentatively characterized by comparing authentic standards or published data. The result showed that this study could provide valuable information for the quality control and further investigation of SHLPI formula. PMID:26177215
Li, Fang; Tan, Yi-Sha; Chen, Hong-Lin; Yan, Yan; Zhai, Ke-Feng; Li, Da-Peng; Kou, Jun-Ping; Yu, Bo-Yang
2015-09-01
YiQiFuMai Powder Injection (YQFM) is a re-developed preparation based on the well-known traditional Chinese medicine formula Sheng-mai-san. It has been widely used for the treatment of cardiovascular disease with definite clinical efficacy in China, but its bioactive molecules remain obscure. In this study, an effective method has been employed as a tool for screening active components in YQFM, using human umbilical vein endothelial cells (HUVECs) extraction and liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS). Nine compounds, which could interact with HUVECs, were identified as ginsenosides Rb1, Rc, Rb2, Rd, 20(S)-Rg3, 20(R)-Rg3, Rk1/Rg5 and schisandrin by comparing with reference substances or literature. In vitro assays showed that schisandrin at concentrations of 10-100 μM protected HUVECs from hypoxia/reoxygenation (H/R) injury, increased cell viability, nitric oxide (NO) content and decreased lactate dehydrogenase (LDH) leakage, malonaldehyde (MDA) content and ROS generation. Moreover, schisandrin pretreatment inhibited cell apoptosis, as evidenced by inhibiting activation of caspase-3 and increasing the Bcl-2/Bax ratio. These data indicate that HUVECs biospecific extraction coupled with HPLC-ESI-Q-TOF-MS/MS analysis is a reliable method for screening potential bioactive components from traditional Chinese medicines. Meanwhile, the vascular endothelium protective property of schisandrin might be beneficial for the treatment of cardiovascular disease. PMID:26452526
Li, Lingzhi; Wang, Zhenzhong; Peng, Ying; Fu, Xiaohuan; Wang, Yongxiang; Xiao, Wei; Song, Shaojiang
2015-01-01
A high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-UV-irradiation (HPLC-QTOF-MS-UV) method was established for rapid separation and structural identification of the constituents in Re Du Ning Injections (RDNI). A total of 20 potentially bioactive compounds including 10 caffeoylquinic acids and 10 iridoid glycosides were identified or tentatively characterized in RDNI by comparing their retention times and MS spectra with those of authentic standards or literature data. In particular, UV-irradiation was employed in the identification of the cis/trans isomers of caffeoylquinic acids. Furthermore, each compound was assigned to the individual raw materials (Artemisia annua L., Lonicera japonica Thunb. or Gardenia jasminoides Ellis) present in RDNI. This is the first time that an HPLC-QTOF-MS-UV analytical method has been used for the identification of caffeoylquinic acids in RDNI. PMID:25265866
Orthogonal polynomials and tolerancing
NASA Astrophysics Data System (ADS)
Rogers, John R.
2011-10-01
Previous papers have established the inadvisability of applying tolerances directly to power-series aspheric coefficients. The basic reason is that the individual terms are far from orthogonal. Zernike surfaces and the new Forbes surface types have certain orthogonality properties over the circle described by the "normalization radius." However, at surfaces away from the stop, the optical beam is smaller than the surface, and the polynomials are not orthogonal over the area sampled by the beam. In this paper, we investigate the breakdown of orthogonality as the surface moves away from the aperture stop, and the implications of this to tolerancing.
Soha, Aria; Chiu, Mickey; Mannel, Eric; Stoll, Sean; Lynch, Don; Boose, Steve; Northacker, Dave; Alfred, Marcus; Lindesay, James; Chujo, Tatsuya; Inaba, Motoi; Nonaka, Toshihiro; Sato, Wataru; Sakatani, Ikumi; Hirano, Masahiro; Choi, Ihnjea
2014-01-15
This is a technical scope of work (TSW) between the Fermi National Accelerator Laboratory (Fermilab) and the experimenters of PHENIX Fast TOF group who have committed to participate in beam tests to be carried out during the FY2014 Fermilab Test Beam Facility program. The goals for this test beam experiment are to verify the timing performance of the two types of time-of-flight detector prototypes.
Orthogonal Regression and Equivariance.
ERIC Educational Resources Information Center
Blankmeyer, Eric
Ordinary least-squares regression treats the variables asymmetrically, designating a dependent variable and one or more independent variables. When it is not obvious how to make this distinction, a researcher may prefer to use orthogonal regression, which treats the variables symmetrically. However, the usual procedure for orthogonal regression is…
Orthogonal tensor decompositions
Tamara G. Kolda
2000-03-01
The authors explore the orthogonal decomposition of tensors (also known as multi-dimensional arrays or n-way arrays) using two different definitions of orthogonality. They present numerous examples to illustrate the difficulties in understanding such decompositions. They conclude with a counterexample to a tensor extension of the Eckart-Young SVD approximation theorem by Leibovici and Sabatier [Linear Algebra Appl. 269(1998):307--329].
Coherent orthogonal polynomials
Celeghini, E.; Olmo, M.A. del
2013-08-15
We discuss a fundamental characteristic of orthogonal polynomials, like the existence of a Lie algebra behind them, which can be added to their other relevant aspects. At the basis of the complete framework for orthogonal polynomials we include thus–in addition to differential equations, recurrence relations, Hilbert spaces and square integrable functions–Lie algebra theory. We start here from the square integrable functions on the open connected subset of the real line whose bases are related to orthogonal polynomials. All these one-dimensional continuous spaces allow, besides the standard uncountable basis (|x〉), for an alternative countable basis (|n〉). The matrix elements that relate these two bases are essentially the orthogonal polynomials: Hermite polynomials for the line and Laguerre and Legendre polynomials for the half-line and the line interval, respectively. Differential recurrence relations of orthogonal polynomials allow us to realize that they determine an infinite-dimensional irreducible representation of a non-compact Lie algebra, whose second order Casimir C gives rise to the second order differential equation that defines the corresponding family of orthogonal polynomials. Thus, the Weyl–Heisenberg algebra h(1) with C=0 for Hermite polynomials and su(1,1) with C=−1/4 for Laguerre and Legendre polynomials are obtained. Starting from the orthogonal polynomials the Lie algebra is extended both to the whole space of the L{sup 2} functions and to the corresponding Universal Enveloping Algebra and transformation group. Generalized coherent states from each vector in the space L{sup 2} and, in particular, generalized coherent polynomials are thus obtained. -- Highlights: •Fundamental characteristic of orthogonal polynomials (OP): existence of a Lie algebra. •Differential recurrence relations of OP determine a unitary representation of a non-compact Lie group. •2nd order Casimir originates a 2nd order differential equation that defines
Reengineering orthogonally selective riboswitches.
Dixon, Neil; Duncan, John N; Geerlings, Torsten; Dunstan, Mark S; McCarthy, John E G; Leys, David; Micklefield, Jason
2010-02-16
The ability to independently control the expression of multiple genes by addition of distinct small-molecule modulators has many applications from synthetic biology, functional genomics, pharmaceutical target validation, through to gene therapy. Riboswitches are relatively simple, small-molecule-dependent, protein-free, mRNA genetic switches that are attractive targets for reengineering in this context. Using a combination of chemical genetics and genetic selection, we have developed riboswitches that are selective for synthetic "nonnatural" small molecules and no longer respond to the natural intracellular ligands. The orthogonal selectivity of the riboswitches is also demonstrated in vitro using isothermal titration calorimetry and x-ray crystallography. The riboswitches allow highly responsive, dose-dependent, orthogonally selective, and dynamic control of gene expression in vivo. It is possible that this approach may be further developed to reengineer other natural riboswitches for application as small-molecule responsive genetic switches in both prokaryotes and eukaryotes. PMID:20133756
Some discrete multiple orthogonal polynomials
NASA Astrophysics Data System (ADS)
Arvesú, J.; Coussement, J.; van Assche, W.
2003-04-01
In this paper, we extend the theory of discrete orthogonal polynomials (on a linear lattice) to polynomials satisfying orthogonality conditions with respect to r positive discrete measures. First we recall the known results of the classical orthogonal polynomials of Charlier, Meixner, Kravchuk and Hahn (T.S. Chihara, An Introduction to Orthogonal Polynomials, Gordon and Breach, New York, 1978; R. Koekoek and R.F. Swarttouw, Reports of the Faculty of Technical Mathematics and Informatics No. 98-17, Delft, 1998; A.F. Nikiforov et al., Classical Orthogonal Polynomials of a Discrete Variable, Springer, Berlin, 1991). These polynomials have a lowering and raising operator, which give rise to a Rodrigues formula, a second order difference equation, and an explicit expression from which the coefficients of the three-term recurrence relation can be obtained. Then we consider r positive discrete measures and define two types of multiple orthogonal polynomials. The continuous case (Jacobi, Laguerre, Hermite, etc.) was studied by Van Assche and Coussement (J. Comput. Appl. Math. 127 (2001) 317-347) and Aptekarev et al. (Multiple orthogonal polynomials for classical weights, manuscript). The families of multiple orthogonal polynomials (of type II) that we will study have a raising operator and hence a Rodrigues formula. This will give us an explicit formula for the polynomials. Finally, there also exists a recurrence relation of order r+1 for these multiple orthogonal polynomials of type II. We compute the coefficients of the recurrence relation explicitly when r=2.
NASA Astrophysics Data System (ADS)
Mehranian, Abolfazl; Kotasidis, Fotis; Zaidi, Habib
2016-02-01
Time-of-flight (TOF) positron emission tomography (PET) technology has recently regained popularity in clinical PET studies for improving image quality and lesion detectability. Using TOF information, the spatial location of annihilation events is confined to a number of image voxels along each line of response, thereby the cross-dependencies of image voxels are reduced, which in turns results in improved signal-to-noise ratio and convergence rate. In this work, we propose a novel approach to further improve the convergence of the expectation maximization (EM)-based TOF PET image reconstruction algorithm through subsetization of emission data over TOF bins as well as azimuthal bins. Given the prevalence of TOF PET, we elaborated the practical and efficient implementation of TOF PET image reconstruction through the pre-computation of TOF weighting coefficients while exploiting the same in-plane and axial symmetries used in pre-computation of geometric system matrix. In the proposed subsetization approach, TOF PET data were partitioned into a number of interleaved TOF subsets, with the aim of reducing the spatial coupling of TOF bins and therefore to improve the convergence of the standard maximum likelihood expectation maximization (MLEM) and ordered subsets EM (OSEM) algorithms. The comparison of on-the-fly and pre-computed TOF projections showed that the pre-computation of the TOF weighting coefficients can considerably reduce the computation time of TOF PET image reconstruction. The convergence rate and bias-variance performance of the proposed TOF subsetization scheme were evaluated using simulated, experimental phantom and clinical studies. Simulations demonstrated that as the number of TOF subsets is increased, the convergence rate of MLEM and OSEM algorithms is improved. It was also found that for the same computation time, the proposed subsetization gives rise to further convergence. The bias-variance analysis of the experimental NEMA phantom and a clinical
Orthogonal Regression: A Teaching Perspective
ERIC Educational Resources Information Center
Carr, James R.
2012-01-01
A well-known approach to linear least squares regression is that which involves minimizing the sum of squared orthogonal projections of data points onto the best fit line. This form of regression is known as orthogonal regression, and the linear model that it yields is known as the major axis. A similar method, reduced major axis regression, is…
NASA Astrophysics Data System (ADS)
Tonry, J.; Burke, Barry E.; Schechter, Paul L.
1997-10-01
We have designed and built a new type of CCD that we call an orthogonal transfer CCD (OTCCD), which permits parallel clocking horizontally as well as vertically. The device has been used successfully to remove image motion caused by atmospheric turbulence at rates up to 100 Hz, and promises to be a better, cheaper way to carry out image motion correction for imaging than by using fast tip/tilt mirrors. We report on the device characteristics, and find that the large number of transfers needed to track image motion does not significantly degrade the image either because of charge transfer inefficiency or because of charge traps. For example, after 100 sec of tracking at 100 Hz approximately 3% of the charge would diffuse into a skirt around the point spread function. Four nights of data at the Michigan-Dartmouth-MIT (MDM) 2.4-m telescope also indicate that the atmosphere is surprisingly benign, in terms of both the speed and coherence angle of image motion. Image motion compensation improved image sharpness by about 0.5'' in quadrature with no degradation over a field of at least 3 arcminutes. (SECTION: Astronomical Instrumentation)
Compact orthogonal NMR field sensor
Gerald, II, Rex E.; Rathke, Jerome W.
2009-02-03
A Compact Orthogonal Field Sensor for emitting two orthogonal electro-magnetic fields in a common space. More particularly, a replacement inductor for existing NMR (Nuclear Magnetic Resonance) sensors to allow for NMR imaging. The Compact Orthogonal Field Sensor has a conductive coil and a central conductor electrically connected in series. The central conductor is at least partially surrounded by the coil. The coil and central conductor are electrically or electro-magnetically connected to a device having a means for producing or inducing a current through the coil and central conductor. The Compact Orthogonal Field Sensor can be used in NMR imaging applications to determine the position and the associated NMR spectrum of a sample within the electro-magnetic field of the central conductor.
Orthogonal polynomials and deformed oscillators
NASA Astrophysics Data System (ADS)
Borzov, V. V.; Damaskinsky, E. V.
2015-10-01
In the example of the Fibonacci oscillator, we discuss the construction of oscillator-like systems associated with orthogonal polynomials. We also consider the question of the dimensions of the corresponding Lie algebras.
"Orthogonality" in Learning and Assessment
ERIC Educational Resources Information Center
Leslie, David
2014-01-01
This chapter proposes a simple framework, "orthogonality," to help clarify what stakeholders think about learning in college, how we assess outcomes, and how clear assessment methods might help increase confidence in returns on investment.
Nonlocality of orthogonal product states
NASA Astrophysics Data System (ADS)
Zhang, Zhi-Chao; Gao, Fei; Qin, Su-Juan; Yang, Ying-Hui; Wen, Qiao-Yan
2015-07-01
In this paper, we mainly study the local indistinguishability of mutually orthogonal product basis quantum states in d ⊗d . In 3 ⊗3 , Bennett et al. [ Phys. Rev. A 59, 1070 (1999), 10.1103/PhysRevA.59.1070] presented nine orthogonal product basis quantum states which cannot be distinguished by local operations and classical communication (LOCC). In the work by Zhang et al. [Z.-C. Zhang et al., Phys. Rev. A 90, 022313 (2014), 10.1103/PhysRevA.90.022313], this result was generalized in d ⊗d , where d is odd. In this paper, we aim to construct locally indistinguishable orthogonal product basis quantum states in d ⊗d . For the general d ⊗d (d >2 ) quantum system, we first construct 4 d -4 orthogonal product states, and prove these states are locally indistinguishable using a very simple but quite effective method. Then, based on these states, we construct some classes of locally indistinguishable orthogonal product basis quantum states (OPBS) in d ⊗d (d >2 ) . Finally, we construct some LOCC indistinguishable OPBS in multipartite quantum systems. All of the above results demonstrate the phenomenon of nonlocality without entanglement.
Biomedical studies by TOF-SIMS imaging.
Bich, Claudia; Touboul, David; Brunelle, Alain
2015-01-01
Imaging by secondary ion mass spectrometry coupled to time-of-flight mass analysis (TOF-SIMS) is a method of which the applications have greatly increased since 10 years. Taking advantage of the development of cluster ion sources, TOF-SIMS offers images of molecular ions at a micrometer lateral resolution or slightly below and does not require complex sample preparation. Although TOF-SIMS has been primarily dedicated to surface analysis of inorganic or polymeric samples, several groups have successfully demonstrated that TOF-SIMS imaging is also perfectly suited for mapping organic compounds, such as drugs or lipids, directly on tissue sections from animals or from human biopsies. This minireview will enlighten some of these developments in the field of biomedical applications. PMID:25708628
Retinal vein occlusion-intravitreal injection; Triamcinolone-intravitreal injection; Dexamethasone-intravitreal injection; Lucentis-intravitreal injection; Avastin-intravitreal injection; Bevacizumab-intravitreal injection; Ranibizumab- ...
Orthogonality preserving infinite dimensional quadratic stochastic operators
Akın, Hasan; Mukhamedov, Farrukh
2015-09-18
In the present paper, we consider a notion of orthogonal preserving nonlinear operators. We introduce π-Volterra quadratic operators finite and infinite dimensional settings. It is proved that any orthogonal preserving quadratic operator on finite dimensional simplex is π-Volterra quadratic operator. In infinite dimensional setting, we describe all π-Volterra operators in terms orthogonal preserving operators.
High sensitive and throughput screening of Aflatoxin using MALDI-TOF-TOF-PSD-MS/MS
Technology Transfer Automated Retrieval System (TEKTRAN)
We have achieved sensitive and efficient detection of aflatoxin B1(AFB1) through matrix-assisted laser desorption/ionization time-of-flight-time-of-flight mass spectrometry (MALDI-TOF-TOF) and post-source decay (PSD) tandem mass spectrometry (MS/MS) using an acetic acid – a-cyano-4-hydroxycinnamic a...
Generalized orthogonal wavelet phase reconstruction.
Axtell, Travis W; Cristi, Roberto
2013-05-01
Phase reconstruction is used for feedback control in adaptive optics systems. To achieve performance metrics for high actuator density or with limited processing capabilities on spacecraft, a wavelet signal processing technique is advantageous. Previous derivations of this technique have been limited to the Haar wavelet. This paper derives the relationship and algorithms to reconstruct phase with O(n) computational complexity for wavelets with the orthogonal property. This has additional benefits for performance with noise in the measurements. We also provide details on how to handle the boundary condition for telescope apertures. PMID:23695316
Time-resolved proper orthogonal decomposition of liquid jet dynamics
NASA Astrophysics Data System (ADS)
Arienti, Marco; Soteriou, Marios C.
2009-11-01
New insight into the mechanism of liquid jet in crossflow atomization is provided by an analysis technique based on proper orthogonal decomposition and spectral analysis. Data are provided in the form of high-speed videos of the jet near field from experiments over a broad range of injection conditions. For each condition, proper orthogonal modes (POMs) are generated and ordered by intensity variation relative to the time average. The feasibility of jet dynamics reduction by truncation of the POM series to the first few modes is then examined as a function of crossflow velocity for laminar and turbulent liquid injection. At conditions where the jet breaks up into large chunks of liquid, the superposition of specific orthogonal modes is observed to track long waves traveling along the liquid column. The temporal coefficients of these modes can be described as a bandpass spectrum that shifts toward higher frequencies as the crossflow velocity is increased. The dynamic correlation of these modes is quantified by their cross-power spectrum density. Based on the frequency and wavelength extracted from the videos, the observed traveling waves are linked to the linearly fastest growing wave of Kelvin-Helmholtz instability. The gas boundary layer thickness at the gas-liquid shear layer emerges at the end of this study as the dominant length scale of jet dynamics at moderate Weber numbers.
Nonambipolarity, orthogonal conductivity, poloidal flow, and torque
Hulbert, G.W.; Perkins, F.W.
1989-02-01
Nonambipolar processes, such as neutral injection onto trapped orbits or ripple-diffusion loss of ..cap alpha..-particles, act to charge a plasma. A current j/sub r/ across magnetic surfaces must arise in the bulk plasma to maintain charge neutrality. An axisymmetric, neoclassical model of the bulk plasma shows that these currents are carried by the ions and exert a j/sub r/B/sub theta/R/c torque in the toroidal direction. A driven poloidal flow V/sub theta/ = E/sub r/'c/B must also develop. The average current density
Orthogonality catastrophe in quantum sticking.
Clougherty, Dennis P; Zhang, Yanting
2012-09-21
We show that the orthogonality catastrophe can dramatically affect the probability with which an ultralow energy atom or ion will stick to a surface. We predict new energy-dependent scaling laws for the sticking probability in this low-energy regime. We provide numerical results of this theory for the case of ultracold electrons sticking to the surface of highly porous silicon and show that the sticking probability can differ substantially from that calculated with perturbation theory. We then generalize our results for finite surface temperatures and find surprisingly that the sticking probability can change sharply, vanishing below a critical incident energy that varies with the surface temperature. We describe in detail this superreflective surface phase for ultralow energy matter waves where the reflection coefficient is strictly equal to one. PMID:23005925
Orthogonality Catastrophe in Quantum Sticking
NASA Astrophysics Data System (ADS)
Clougherty, Dennis P.; Zhang, Yanting
2012-09-01
We show that the orthogonality catastrophe can dramatically affect the probability with which an ultralow energy atom or ion will stick to a surface. We predict new energy-dependent scaling laws for the sticking probability in this low-energy regime. We provide numerical results of this theory for the case of ultracold electrons sticking to the surface of highly porous silicon and show that the sticking probability can differ substantially from that calculated with perturbation theory. We then generalize our results for finite surface temperatures and find surprisingly that the sticking probability can change sharply, vanishing below a critical incident energy that varies with the surface temperature. We describe in detail this superreflective surface phase for ultralow energy matter waves where the reflection coefficient is strictly equal to one.
Orthogonal separations: Comparison of orthogonality metrics by statistical analysis.
Schure, Mark R; Davis, Joe M
2015-10-01
Twenty orthogonality metrics (OMs) derived from convex hull, information theory, fractal dimension, correlation coefficients, nearest neighbor distances and bin-density techniques were calculated from a diverse group of 47 experimental two-dimensional (2D) chromatograms. These chromatograms comprise two datasets; one dataset is a collection of 2D chromatograms from Peter Carr's laboratory at the University of Minnesota, and the other dataset is based on pairs of one-dimensional chromatograms previously published by Martin Gilar and coworkers (Waters Corp.). The chromatograms were pooled to make a third or combined dataset. Cross-correlation results suggest that specific OMs are correlated within families of nearest neighbor methods, correlation coefficients and the information theory methods. Principal component analysis of the OMs show that none of the OMs stands out as clearly better at explaining the data variance than any another OM. Principal component analysis of individual chromatograms shows that different OMs favor certain chromatograms. The chromatograms exhibit a range of quality, as subjectively graded by nine experts experienced in 2D chromatography. The subjective (grading) evaluations were taken at two intervals per expert and demonstrated excellent consistency for each expert. Excellent agreement for both very good and very bad chromatograms was seen across the range of experts. However, evaluation uncertainty increased for chromatograms that were judged as average to mediocre. The grades were converted to numbers (percentages) for numerical computations. The percentages were correlated with OMs to establish good OMs for evaluating the quality of 2D chromatograms. Certain metrics correlate better than others. However, these results are not consistent across all chromatograms examined. Most of the nearest neighbor methods were observed to correlate poorly with the percentages. However, one method, devised by Clark and Evans, appeared to work
MAP reconstruction for Fourier rebinned TOF-PET data
NASA Astrophysics Data System (ADS)
Bai, Bing; Lin, Yanguang; Zhu, Wentao; Ren, Ran; Li, Quanzheng; Dahlbom, Magnus; DiFilippo, Frank; Leahy, Richard M.
2014-02-01
Time-of-flight (TOF) information improves the signal-to-noise ratio in positron emission tomography (PET). The computation cost in processing TOF-PET sinograms is substantially higher than for nonTOF data because the data in each line of response is divided among multiple TOF bins. This additional cost has motivated research into methods for rebinning TOF data into lower dimensional representations that exploit redundancies inherent in TOF data. We have previously developed approximate Fourier methods that rebin TOF data into either three-dimensional (3D) nonTOF or 2D nonTOF formats. We refer to these methods respectively as FORET-3D and FORET-2D. Here we describe maximum a posteriori (MAP) estimators for use with FORET rebinned data. We first derive approximate expressions for the variance of the rebinned data. We then use these results to rescale the data so that the variance and mean are approximately equal allowing us to use the Poisson likelihood model for MAP reconstruction. MAP reconstruction from these rebinned data uses a system matrix in which the detector response model accounts for the effects of rebinning. Using these methods we compare the performance of FORET-2D and 3D with TOF and nonTOF reconstructions using phantom and clinical data. Our phantom results show a small loss in contrast recovery at matched noise levels using FORET compared to reconstruction from the original TOF data. Clinical examples show FORET images that are qualitatively similar to those obtained from the original TOF-PET data but with a small increase in variance at matched resolution. Reconstruction time is reduced by a factor of 5 and 30 using FORET3D+MAP and FORET2D+MAP respectively compared to 3D TOF MAP, which makes these methods attractive for clinical applications.
Experimental stability maps of a 1550nm-VCSEL subject to polarized optical injection
NASA Astrophysics Data System (ADS)
Hurtado, A.; Schires, K.; Khan, N.; Quirce, A.; Valle, A.; Pesquera, L.; Henning, I. D.; Adams, M. J.
2010-02-01
We report an experimental study of the injection locking properties and nonlinear dynamics of a 1550nm-Vertical Cavity Surface Emitting Laser (VCSEL) subject to parallel and to orthogonal polarized optical injection into the two orthogonal polarizations of the fundamental transverse mode of the device. A rich variety of nonlinear dynamics have been observed for both parallel and orthogonal optical injection outside the stable locking range, including limit cycle, period doubling, chaos and polarization switching. Additionally, for the case of orthogonal optical injection, we report a first experimental observation of the change in the shape of the stability map as the applied bias current is increased well above threshold.
Cherenkov TOF PET with silicon photomultipliers
NASA Astrophysics Data System (ADS)
Dolenec, R.; Korpar, S.; Križan, P.; Pestotnik, R.
2015-12-01
As previously demonstrated, an excellent timing resolution below 100 ps FWHM is possible in time-of-flight positron emission tomography (TOF PET) if the detection method is based on the principle of detecting photons of Cherenkov light, produced in a suitable material and detected by microchannel plate photomultipliers (MCP PMTs). In this work, the silicon photomultipliers (SiPMs) were tested for the first time as the photodetectors in Cherenkov TOF PET. The high photon detection efficiency (PDE) of SiPMs led to a large improvement in detection efficiency. On the other hand, the time response of currently available SiPMs is not as good as that of MCP PMTs. The SiPM dark counts introduce a new source of random coincidences in Cherenkov method, which would be overwhelming with present SiPM technology at room temperature. When the apparatus was cooled, its performance significantly improved.
Characterization of honeybee venom by MALDI-TOF and nanoESI-QqTOF mass spectrometry.
Matysiak, Jan; Schmelzer, Christian E H; Neubert, Reinhard H H; Kokot, Zenon J
2011-01-25
The aim of the study was to comprehensively characterize different honeybee venom samples applying two complementary mass spectrometry methods. 41 honeybee venom samples of different bee strains, country of origin (Poland, Georgia, and Estonia), year and season of the venom collection were analyzed using MALDI-TOF and nanoESI-QqTOF-MS. It was possible to obtain semi-quantitative data for 12 different components in selected honeybee venom samples using MALDI-TOF method without further sophisticated and time consuming sample pretreatment. Statistical analysis (ANOVA) has shown that there are qualitative and quantitative differences in the composition between honeybee venom samples collected over different years. It has also been demonstrated that MALDI-TOF spectra can be used as a "protein fingerprint" of honeybee venom in order to confirm the identity of the product. NanoESI-QqTOF-MS was applied especially for identification purposes. Using this technique 16 peptide sequences were identified, including melittin (12 different breakdown products and precursors), apamine, mast cell degranulating peptide and secapin. Moreover, the significant achievement of this study is the fact that the new peptide (HTGAVLAGV+Amidated (C-term), M(r)=822.53Da) has been discovered in bee venom for the first time. PMID:20850943
[Orthogonal Vector Projection Algorithm for Spectral Unmixing].
Song, Mei-ping; Xu, Xing-wei; Chang, Chein-I; An, Ju-bai; Yao, Li
2015-12-01
Spectrum unmixing is an important part of hyperspectral technologies, which is essential for material quantity analysis in hyperspectral imagery. Most linear unmixing algorithms require computations of matrix multiplication and matrix inversion or matrix determination. These are difficult for programming, especially hard for realization on hardware. At the same time, the computation costs of the algorithms increase significantly as the number of endmembers grows. Here, based on the traditional algorithm Orthogonal Subspace Projection, a new method called. Orthogonal Vector Projection is prompted using orthogonal principle. It simplifies this process by avoiding matrix multiplication and inversion. It firstly computes the final orthogonal vector via Gram-Schmidt process for each endmember spectrum. And then, these orthogonal vectors are used as projection vector for the pixel signature. The unconstrained abundance can be obtained directly by projecting the signature to the projection vectors, and computing the ratio of projected vector length and orthogonal vector length. Compared to the Orthogonal Subspace Projection and Least Squares Error algorithms, this method does not need matrix inversion, which is much computation costing and hard to implement on hardware. It just completes the orthogonalization process by repeated vector operations, easy for application on both parallel computation and hardware. The reasonability of the algorithm is proved by its relationship with Orthogonal Sub-space Projection and Least Squares Error algorithms. And its computational complexity is also compared with the other two algorithms', which is the lowest one. At last, the experimental results on synthetic image and real image are also provided, giving another evidence for effectiveness of the method. PMID:26964231
On orthogonality preserving quadratic stochastic operators
Mukhamedov, Farrukh; Taha, Muhammad Hafizuddin Mohd
2015-05-15
A quadratic stochastic operator (in short QSO) is usually used to present the time evolution of differing species in biology. Some quadratic stochastic operators have been studied by Lotka and Volterra. In the present paper, we first give a simple characterization of Volterra QSO in terms of absolutely continuity of discrete measures. Further, we introduce a notion of orthogonal preserving QSO, and describe such kind of operators defined on two dimensional simplex. It turns out that orthogonal preserving QSOs are permutations of Volterra QSO. The associativity of genetic algebras generated by orthogonal preserving QSO is studied too.
Analytic TOF PET reconstruction algorithm within DIRECT data partitioning framework
NASA Astrophysics Data System (ADS)
Matej, Samuel; Daube-Witherspoon, Margaret E.; Karp, Joel S.
2016-05-01
Iterative reconstruction algorithms are routinely used for clinical practice; however, analytic algorithms are relevant candidates for quantitative research studies due to their linear behavior. While iterative algorithms also benefit from the inclusion of accurate data and noise models the widespread use of time-of-flight (TOF) scanners with less sensitivity to noise and data imperfections make analytic algorithms even more promising. In our previous work we have developed a novel iterative reconstruction approach (DIRECT: direct image reconstruction for TOF) providing convenient TOF data partitioning framework and leading to very efficient reconstructions. In this work we have expanded DIRECT to include an analytic TOF algorithm with confidence weighting incorporating models of both TOF and spatial resolution kernels. Feasibility studies using simulated and measured data demonstrate that analytic-DIRECT with appropriate resolution and regularization filters is able to provide matched bias versus variance performance to iterative TOF reconstruction with a matched resolution model.
Fragmentation of Multiply-Charged Intact Protein Ions Using MALDI TOF-TOF Mass Spectrometry
Liu, Zhaoyang; Schey, Kevin L.
2008-01-01
Top down proteomics in a TOF-TOF instrument was further explored by examining the fragmentation of multiply charged precursors ions generated by matrix-assisted laser desorption ionization. Evaluation of sample preparation conditions allowed selection of solvent/matrix conditions and sample deposition methods to produce sufficiently abundant doubly and triply charged precursor ions for subsequent CID experiments. As previously reported, preferential cleavage was observed at sites C-terminal to acidic residues and N-terminal to proline residues for all ions examined. An increase in non-preferential fragmentation as well as additional low mass product ions was observed in the spectra from multiply charged precursor ions providing increased sequence coverage. This enhanced fragmentation from multiply charged precursor ions became increasingly important with increasing protein molecular weight and facilitates protein identification using database searching algorithms. The useable mass range for MALDI TOF-TOF analysis of intact proteins has been expanded to 18.2 kDa using this approach. PMID:17693096
Chen Pingxing; Li Chengzu
2003-12-01
We consider the relation between the orthogonality and the distinguishability of a set of arbitrary states (including multipartite states). It is shown that if a set of arbitrary states can be distinguished by local operations and classical communication (LOCC), each of the states can be written as a linear combination of product vectors such that all product vectors of one of the states are orthogonal to the other states. With this result we then prove a simple necessary condition for LOCC distinguishability of a class of orthogonal states. These conclusions may be useful in discussing the distinguishability of orthogonal quantum states further, understanding the essence of nonlocality and discussing the distillation of entanglement.
NASA Astrophysics Data System (ADS)
Warneke, C.; Veres, P.; Murphy, S. M.; Soltis, J.; Field, R. A.; Graus, M. G.; Koss, A.; Li, S.-M.; Li, R.; Yuan, B.; Roberts, J. M.; de Gouw, J. A.
2015-01-01
Here we compare volatile organic compound (VOC) measurements using a standard proton-transfer-reaction quadrupole mass spectrometer (PTR-QMS) with a new proton-transfer-reaction time of flight mass spectrometer (PTR-TOF) during the Uintah Basin Winter Ozone Study 2013 (UBWOS2013) field experiment in an oil and gas field in the Uintah Basin, Utah. The PTR-QMS uses a quadrupole, which is a mass filter that lets one mass to charge ratio pass at a time, whereas the PTR-TOF uses a time of flight mass spectrometer, which takes full mass spectra with typical 0.1 s-1 min integrated acquisition times. The sensitivity of the PTR-QMS in units of counts per ppbv (parts per billion by volume) is about a factor of 10-35 times larger than the PTR-TOF, when only one VOC is measured. The sensitivity of the PTR-TOF is mass dependent because of the mass discrimination caused by the sampling duty cycle in the orthogonal-acceleration region of the TOF. For example, the PTR-QMS on mass 33 (methanol) is 35 times more sensitive than the PTR-TOF and for masses above 120 amu less than 10 times more. If more than 10-35 compounds are measured with PTR-QMS, the sampling time per ion decreases and the PTR-TOF has higher signals per unit measuring time for most masses. For UBWOS2013 the PTR-QMS measured 34 masses in 37 s and on that timescale the PTR-TOF is more sensitive for all masses. The high mass resolution of the TOF allows for the measurements of compounds that cannot be separately detected with the PTR-QMS, such as oxidation products from alkanes and cycloalkanes emitted by oil and gas extraction. PTR-TOF masses do not have to be preselected, allowing for identification of unanticipated compounds. The measured mixing ratios of the two instruments agreed very well (R2 ≥ 0.92 and within 20%) for all compounds and masses monitored with the PTR-QMS.
NASA Astrophysics Data System (ADS)
Warneke, C.; Veres, P. R.; Murphy, S. M.; Soltis, J.; Field, R. A.; Graus, M. G.; Koss, A.; Li, S.-M.; Li, R.; Yuan, B.; Roberts, J. M.; de Gouw, J. A.
2014-07-01
Here we compare volatile organic compound (VOC) measurements using a standard Proton-Transfer-Reaction Quadrupole Mass Spectrometer (PTR-QMS) with a new Proton-Transfer-Reaction Time Of Flight Mass Spectrometer (PTR-TOF) during the Uintah Basin Winter Ozone Study 2013 (UBWOS2013) field experiment in an oil and gas field in the Uintah Basin, Utah. The PTR-QMS uses a quadrupole, which is a mass filter that lets one mass pass at a time, whereas the PTR-TOF uses a Time Of Flight mass spectrometer, which takes full mass spectra with typical 0.1 s to 1 min integrated acquisition times. The sensitivity of the PTR-QMS in units of counts per ppbv is about a factor of 10-35 times larger than the PTR-TOF, when only one VOC is measured. The sensitivity of the PTR-TOF is mass dependent because of the mass discrimination caused by the sampling duty cycle in the orthogonal-acceleration region of the TOF. For example, the PTR-QMS on mass 33 (methanol) is 35 times more sensitive than the PTR-TOF and for masses above 120 amu less than 10 times more. If more than 10-35 compounds are measured with PTR-QMS, the sampling time per ion decreases and the PTR-TOF has higher signals per unit measuring time for most masses. For UBWOS2013 the PTR-QMS measured 34 masses in 37 s and on that time-scale the PTR-TOF is more sensitive for all masses. The high mass resolution of the TOF allows for the measurements of compounds that cannot be separately detected with the PTR-QMS, such as oxidation products from alkanes and cycloalkanes emitted by oil and gas extraction. PTR-TOF masses do not have to be pre-selected allowing for identification of unanticipated compounds. The measured mixing ratios of the two instruments agreed very well (R2 ≥ 0.92 and within 20%) for all compounds and masses monitored with the PTR-QMS.
MALDI-TOF MS in Prenatal Genomics
Zhong, Xiao Yan; Holzgreve, Wolfgang
2009-01-01
Summary Prenatal diagnosis aims either to provide the reassurance to the couples at risk of having an affected child by timely appropriate therapy or to give the parents a chance to decide the fate of the unborn babies with health problems. Invasive prenatal diagnosis (IPD) is accurate, however, carrying a risk of miscarriage. Non-invasive prenatal diagnosis (NIPD) has been developed based on the existing of fetal genetic materials in maternal circulation; however, a minority fetal DNA in majority maternal background DNA hinders the detections of fetal traits. Different protocols and assays, such as homogenous MassEXTEND (hME), single allele base extension reaction (SABER), precise measuring copy number variation of each allele, and quantitative methylation and expression analysis using the high-throughput sensitive matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), allow NIPD for single gene disorders, fetal blood group genotyping and fetal aneuploidies as well as the development of fetal gender-independent biomarkers in maternal circulation for management of pathological pregnancies. In this review, we summarise the use of MALDI-TOF MS in prenatal genomics. PMID:21049077
... it.Golimumab injection comes in prefilled syringes and auto-injection devices for subcutaneous injection. Use each syringe ... method.Do not remove the cap from the auto-injection device or the cover from the prefilled ...
Technology Transfer Automated Retrieval System (TEKTRAN)
Matrix-assisted laser desorption/ionization time-of-flight-time-of-flight mass spectrometry(MALDI-TOF-TOF-MS)has provided new capabilities for the rapid identification of digested and non-digested proteins. The tandem (MS/MS) capability of TOF-TOF instruments allows precursor ion selection/isolation...
Face hallucination using orthogonal canonical correlation analysis
NASA Astrophysics Data System (ADS)
Zhou, Huiling; Lam, Kin-Man
2016-05-01
A two-step face-hallucination framework is proposed to reconstruct a high-resolution (HR) version of a face from an input low-resolution (LR) face, based on learning from LR-HR example face pairs using orthogonal canonical correlation analysis (orthogonal CCA) and linear mapping. In the proposed algorithm, face images are first represented using principal component analysis (PCA). Canonical correlation analysis (CCA) with the orthogonality property is then employed, to maximize the correlation between the PCA coefficients of the LR and the HR face pairs to improve the hallucination performance. The original CCA does not own the orthogonality property, which is crucial for information reconstruction. We propose using orthogonal CCA, which is proven by experiments to achieve a better performance in terms of global face reconstruction. In addition, in the residual-compensation process, a linear-mapping method is proposed to include both the inter- and intrainformation about manifolds of different resolutions. Compared with other state-of-the-art approaches, the proposed framework can achieve a comparable, or even better, performance in terms of global face reconstruction and the visual quality of face hallucination. Experiments on images with various parameter settings and blurring distortions show that the proposed approach is robust and has great potential for real-world applications.
Triacylglycerol profile in cocoa liquors using MALDI-TOF and LC-ESI tandem mass spectrometry.
Bono, Luca; Seraglia, Roberta; Roverso, Marco; Di Carro, Marina; Magi, Emanuele
2014-09-01
Triacylglycerols are responsible for chocolate's peculiar melting behavior: the type and position of fatty acids on the glycerol molecule strongly affect the melting range of cocoa butter. For this reason, the characterization of triglyceride composition in cocoa products is particularly important. In this work, triacylglycerols extracted from cocoa liquor samples were analyzed by matrix-assisted laser desorption/ionization time-of-flight (TOF) and electrospray ionization tandem mass spectrometry (MS/MS) coupled to liquid chromatography. Extracted samples were initially analyzed by direct injection in MS to obtain information on triglyceride molecular weights; relevant MS parameters were optimized, and the possible formation of the adducts [M + Na](+) and [M + NH(4)](+) was studied. Tandem mass experiments (both with triple quadrupole and TOF/TOF) were performed to study the fragmentation pathways (in particular, the loss of palmitic, stearic and oleic acid) and identify the triacylglycerols in cocoa liquors. Some signals of the spectra obtained with both MS techniques could indicate the presence of diacylglycerols in the cocoa extract, but different experimental evidences demonstrated that they were generated by the in-source fragmentation of triglycerides. A nonaqueous reversed-phase chromatographic separation was also developed and used to support the identification of the analytes; nine triacylglycerols were recognized in the cocoa liquor extracts. The three different batches of Ecuador cocoa liquor did not show significant differences in the triacylglycerol profile. PMID:25230186
Nonlocality of orthogonal product basis quantum states
NASA Astrophysics Data System (ADS)
Zhang, Zhi-Chao; Gao, Fei; Tian, Guo-Jing; Cao, Tian-Qing; Wen, Qiao-Yan
2014-08-01
In this paper, we mainly study the local indistinguishability of mutually orthogonal product basis quantum states in the high-dimensional quantum systems. In the Hilbert space of 3⊗3, Walgate and Hardy [Phys. Rev. Lett. 89, 147901 (2002), 10.1103/PhysRevLett.89.147901] presented a very simple proof for nonlocality of nine orthogonal product basis quantum states which are given by Bennett et al. [Phys. Rev. A 59, 1070 (1999), 10.1103/PhysRevA.59.1070]. In the quantum system of d⊗d, where d is odd, we construct d2 orthogonal product basis quantum states and prove these states are locally indistinguishable. Then we are able to construct some locally indistinguishable product basis quantum states in the multipartite systems. All these results reveal the phenomenon of "nonlocality without entanglement."
Orthogonal rational functions and tridiagonal matrices
NASA Astrophysics Data System (ADS)
Bultheel, A.; González-Vera, P.; Hendriksen, E.; Njåstad, O.
2003-04-01
We study the recurrence relation for rational functions whose poles are in a prescribed sequence of numbers that are real or infinite and that are orthogonal with respect to an Hermitian positive linear functional. We especially discuss the interplay between finite and infinite poles. The recurrence relation will also be described in terms of a tridiagonal matrix which is a generalization of the Jacobi. matrix of the polynomial situation which corresponds to placing all the poles at infinity. This matrix not only describes the recurrence relation, but it can be used to give a determinant expression for the orthogonal rational functions and it also allows for the formulation of a generalized eigenvalue problem whose eigenvalues are the zeros of an orthogonal rational function. These nodes can be used in rational Gauss-type quadrature formulas and the corresponding weights can be obtained from the first components of the corresponding eigenvectors.
Cheawchan, Sumitra; Uchida, Satoshi; Sogawa, Hiromitsu; Koyama, Yasuhito; Takata, Toshikazu
2016-01-12
The thermotriggered modification of surfaces was performed under catalyst-free conditions using an orthogonal agent possessing both nitrile N-oxide and Meldrum's acid moieties. The nitrile N-oxide moiety of the orthogonal agent successfully underwent catalyst-free 1,3-dipolar cycloaddition to unsaturated bonds of glass surfaces to produce Meldrum's acid-functionalized surfaces. The subsequent thermal decomposition of Meldrum's acid moiety in the presence of nucleophiles afforded versatile nucleophile-modified surfaces (e.g., wet, waterproof, and photoactive surfaces). Surface characteristics were investigated with the water contact angle, time-of-flight secondary ion mass spectrometry (TOF-SIMS), and X-ray photoelectron spectroscopy (XPS). In addition, the surface modification of silica nanoparticles using the orthogonal agent was also achieved to evaluate the density of the functional group concentration on the surface. PMID:26652940
MALDI-TOF and cluster-TOF-SIMS imaging of Fabry disease biomarkers
NASA Astrophysics Data System (ADS)
Touboul, David; Roy, Sandrine; Germain, Dominique P.; Chaminade, Pierre; Brunelle, Alain; Laprevote, Olivier
2007-02-01
Fabry disease is an X-linked disorder of glycosphingolipid metabolism, in which a partial or total deficiency of [alpha]-galactosidase A, a lysosomal enzyme, results in the progressive accumulation of neutral glycosphingolipids (globotriaosylceramide and digalactosylceramide) in most fluids and tissues of the body. Few information is available about the composition and distribution in tissues of the accumulated glycosphingolipids species. Mass spectrometry imaging is an innovative technique, which can provide pieces of information about the distribution of numerous biological compounds, such as lipids, directly on the tissue sections. MALDI-TOF and cluster-TOF-SIMS imaging approaches were used to study the localization of lipids (cholesterol, cholesterol sulfate, vitamin E, glycosphingolipids ...) on skin and kidney sections of patients affected by the Fabry disease. Numerous information on pathophysiology were enlightened by both techniques.
MALDI-TOF/TOF CID Study of Poly(1,4-dihydroxybenzene terephthalate) Fragmentation Reactions
Gies, Anthony P.; Stow, Sarah M.; McLean, John A.; Hercules, David M.
2015-01-01
MALDI-TOF/TOF collision-induced dissociation (CID) experiments were conducted on model aromatic polyester oligomers. CID fragmentation studies identified initial fracture of the ester bond and subsequent CO loss as a major pathway, consistent with the general fragmentation mechanism used to explain the origin of poly(p-phenylenediamine terephthalamide) (PPD-T) fragment ions. Specifically, both charge-remote and charge-site fragmentation were observed. Different parent-ion species were observed, the major ones being carboxyl-hydroxyl, di-carboxyl, di-hydroxyl, and phenyl-carboxyl terminated. One species observed was hydroxyl-diethylamine terminated caused by reaction of carboxyl groups with triethylamine added to the synthesis reaction mixture. Fragment ions reflected the end groups of the parent oligomers. Some MALDI fragment-ion spectra were obtained for species showing exchange between Li and H at the carboxyl end group. Bond energy calculations provide further insight into suggested fragmentation mechanisms. PMID:26195848
Technology Transfer Automated Retrieval System (TEKTRAN)
Keratin was extracted from wool by reduction with 2-mercaptoethanol. It was isolated as intact keratin and characterized by its similar molecular weight, protein composition, and secondary structure to native keratin. Gel electrophoresis patterns and MALDI-TOF/TOF peptide sequences provided the ide...
Technology Transfer Automated Retrieval System (TEKTRAN)
This chapter describes a step-by-step protocol and discussion of top-down proteomic identification of protein biomarkers of food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed in the Pro...
Actinobaculum schaalii: identification with MALDI-TOF
Tuuminen, T; Suomala, P; Harju, I
2014-01-01
Actinobaculum schaalii is an emerging uropathogen. So far, its identification has been performed with 16S rRNA gene sequencing or PCR. The diagnosis has often been delayed due to fastidious growth and identification problems. Eleven clinical isolates of A. schaalii from bloodstream infections that were initially identified with 16S rRNA sequencing analysis were recovered and later identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF). We present a review of bacteriological data of these patients, an algorithm for fast laboratory work-up and advocate the use of sensitized culture of urine to allow better recovery of A. schaalii in susceptible patients. PMID:25356339
Improved piecewise orthogonal signal correction algorithm.
Feudale, Robert N; Tan, Huwei; Brown, Steven D
2003-10-01
Piecewise orthogonal signal correction (POSC), an algorithm that performs local orthogonal filtering, was recently developed to process spectral signals. POSC was shown to improve partial leastsquares regression models over models built with conventional OSC. However, rank deficiencies within the POSC algorithm lead to artifacts in the filtered spectra when removing two or more POSC components. Thus, an updated OSC algorithm for use with the piecewise procedure is reported. It will be demonstrated how the mathematics of this updated OSC algorithm were derived from the previous version and why some OSC versions may not be as appropriate to use with the piecewise modeling procedure as the algorithm reported here. PMID:14639746
Nonlinear Submodels Of Orthogonal Linear Models
ERIC Educational Resources Information Center
Bechtel, Gordon G.
1973-01-01
It is the purpose of this paper to suggest the orthogonal analysis of variance as a device for simplifying either the analytic or iterative problem of finding LS (least squares) estimates for the parameters of particular nonlinear models. (Author/RK)
Multipartite invariant states. II. Orthogonal symmetry
Chruscinski, Dariusz; Kossakowski, Andrzej
2006-06-15
We construct a class of multipartite states possessing orthogonal symmetry. This new class contains multipartite states which are invariant under the action of local unitary operations introduced in our preceding paper [Phys. Rev. A 73, 062314 (2006)]. We study basic properties of multipartite symmetric states: separability criteria and multi-PPT conditions.
The Rigid Orthogonal Procrustes Rotation Problem
ERIC Educational Resources Information Center
ten Berge, Jos M. F.
2006-01-01
The problem of rotating a matrix orthogonally to a best least squares fit with another matrix of the same order has a closed-form solution based on a singular value decomposition. The optimal rotation matrix is not necessarily rigid, but may also involve a reflection. In some applications, only rigid rotations are permitted. Gower (1976) has…
Border separation for adjacent orthogonal fields
Werner, B.L.; Khan, F.M.; Sharma, S.C.; Lee, C.K.; Kim, T.H. )
1991-06-01
Field border separations for adjacent orthogonal fields can be calculated geometrically, given the validity of some important assumptions such as beam alignment and field uniformity. Thermoluminescent dosimetry (TLD) measurements were used to investigate dose uniformity across field junctions as a function of field separation and, in particular, to review the CCSG recommendation for the treatment of medulloblastoma with separate head and spine fields.
Three-Dimensional Orthogonal Co-ordinates
ERIC Educational Resources Information Center
Astin, J.
1974-01-01
A systematic approach to general orthogonal co-ordinates, suitable for use near the end of a beginning vector analysis course, is presented. It introduces students to tensor quantities and shows how equations and quantities needed in classical problems can be determined. (Author/LS)
A class of orthogonal nonrecursive binomial filters.
NASA Technical Reports Server (NTRS)
Haddad, R. A.
1971-01-01
The time- and frequency-domain properties of the orthogonal binomial sequences are presented. It is shown that these sequences, or digital filters based on them, can be generated using adders and delay elements only. The frequency-domain behavior of these nonrecursive binomial filters suggests a number of applications as low-pass Gaussian filters or as inexpensive bandpass filters.
Gram-Schmidt Orthogonalization by Gauss Elimination.
ERIC Educational Resources Information Center
Pursell, Lyle; Trimble, S. Y.
1991-01-01
Described is the hand-calculation method for the orthogonalization of a given set of vectors through the integration of Gaussian elimination with existing algorithms. Although not numerically preferable, this method adds increased precision as well as organization to the solution process. (JJK)
Orthogonal Thin Film Photovoltaics on Vertical Nanostructures.
Ahnood, Arman; Zhou, H; Suzuki, Y; Sliz, R; Fabritius, T; Nathan, Arokia; Amaratunga, G A J
2015-12-01
Decoupling paths of carrier collection and illumination within photovoltaic devices is one promising approach for improving their efficiency by simultaneously increasing light absorption and carrier collection efficiency. Orthogonal photovoltaic devices are core-shell type structures consisting of thin film photovoltaic stack on vertical nanopillar scaffolds. These types of devices allow charge collection to take place in the radial direction, perpendicular to the path of light in the vertical direction. This approach addresses the inherently high recombination rate of disordered thin films, by allowing semiconductor films with minimal thicknesses to be used in photovoltaic devices, without performance degradation associated with incomplete light absorption. This work considers effects which influence the performance of orthogonal photovoltaic devices. Illumination non-uniformity as light travels across the depth of the pillars, electric field enhancement due to the nanoscale size and shape of the pillars, and series resistance due to the additional surface structure created through the use of pillars are considered. All of these effects influence the operation of orthogonal solar cells and should be considered in the design of vertically nanostructured orthogonal photovoltaics. PMID:26676997
Orthogonal Thin Film Photovoltaics on Vertical Nanostructures
NASA Astrophysics Data System (ADS)
Ahnood, Arman; Zhou, H.; Suzuki, Y.; Sliz, R.; Fabritius, T.; Nathan, Arokia; Amaratunga, G. A. J.
2015-12-01
Decoupling paths of carrier collection and illumination within photovoltaic devices is one promising approach for improving their efficiency by simultaneously increasing light absorption and carrier collection efficiency. Orthogonal photovoltaic devices are core-shell type structures consisting of thin film photovoltaic stack on vertical nanopillar scaffolds. These types of devices allow charge collection to take place in the radial direction, perpendicular to the path of light in the vertical direction. This approach addresses the inherently high recombination rate of disordered thin films, by allowing semiconductor films with minimal thicknesses to be used in photovoltaic devices, without performance degradation associated with incomplete light absorption. This work considers effects which influence the performance of orthogonal photovoltaic devices. Illumination non-uniformity as light travels across the depth of the pillars, electric field enhancement due to the nanoscale size and shape of the pillars, and series resistance due to the additional surface structure created through the use of pillars are considered. All of these effects influence the operation of orthogonal solar cells and should be considered in the design of vertically nanostructured orthogonal photovoltaics.
Minakata, Kayoko; Nozawa, Hideki; Yamagishi, Itaru; Gonmori, Kunio; Suzuki, Masako; Hasegawa, Koutaro; Wurita, Amin; Watanabe, Kanako; Suzuki, Osamu
2014-02-01
A rapid determination method is presented for gold (Au(3+)) and platinum (Pt(4+)) in tissues using matrix-assisted laser desorption ionization quadrupole time-of-flight mass spectrometry (MALDI-Q-TOF-MS). Au and Pt ions in wet-ashed tissue solution were reacted with diethyldithiocarbamate (DDC), and the resulting chelate complex ions Au(DDC)2 (+) and Pt(DDC)3 (+) were detected by MALDI-Q-TOF-MS using α-cyano-4-hydroxycinnamic acid as a matrix. The limit of detection (LOD) was 0.8 ng/g tissue and the quantification range was 2-400 ng/g for Au, and the LOD was 6 ng/g tissue and the quantification range was 20-4,000 ng/g for Pt. The Pt levels detected by MALDI-Q-TOF-MS in several tissues of a patient overdosed with cisplatin were nearly the same as those detected by flow-injection electrospray ionization mass spectrometry. The LODs of Au and Pt were 0.04 pg per well (sample spot) and 0.3 pg per well, respectively. To our knowledge, this is the first attempt to quantify Au(3+) and Pt(4+) ions in tissues by MALDI-Q-TOF-MS. PMID:23455689
... at golimumab injection before injecting it. Check the expiration date printed on the auto-injection device or carton and do not use the medication if the expiration date has passed. Do not use a prefilled syringe ...
SS{sub p}G: A strongly orthogonal geminal method with relaxed strong orthogonality
Cagg, Brett A. Rassolov, Vitaly A.
2014-10-28
Strong orthogonality is an important constraint placed on geminal wavefunctions in order to make variational minimization tractable. However, strong orthogonality prevents certain, possibly important, excited configurations from contributing to the ground state description of chemical systems. The presented method lifts strong orthogonality constraint from geminal wavefunction by computing a perturbative-like correction to each geminal independently from the corrections to all other geminals. The method is applied to the Singlet-type Strongly orthogonal Geminals variant of the geminal wavefunction. Comparisons of this new SS{sub p}G method are made to the non-orthogonal AP1roG and the unconstrained Geminal Mean-Field Configuration Interaction method using small atomic and molecular systems. The correction is also compared to Density Matrix Renormalization Group calculations performed on long polyene chains in order to assess its scalability and applicability to large strongly correlated systems. The results of these comparisons demonstrate that although the perturbative correction is small, it may be a necessary first step in the systematic improvement of any strongly orthogonal geminal method.
NASA Astrophysics Data System (ADS)
Wang, Jie; Liang, Xingdong; Chen, Longyong; Ding, Chibiao
2015-01-01
Orthogonal frequency division multiplexing (OFDM) chirp waveform, which is composed of two successive identical linear frequency modulated subpulses, is a newly proposed orthogonal waveform scheme for multiinput multioutput synthetic aperture radar (SAR) systems. However, according to the waveform model, radar systematic error, which introduces phase or amplitude difference between the subpulses of the OFDM waveform, significantly degrades the orthogonality. The impact of radar systematic error on the waveform orthogonality is mainly caused by the systematic nonlinearity rather than the thermal noise or the frequency-dependent systematic error. Due to the influence of the causal filters, the first subpulse leaks into the second one. The leaked signal interacts with the second subpulse in the nonlinear components of the transmitter. This interaction renders a dramatic phase distortion in the beginning of the second subpulse. The resultant distortion, which leads to a phase difference between the subpulses, seriously damages the waveform's orthogonality. The impact of radar systematic error on the waveform orthogonality is addressed. Moreover, the impact of the systematic nonlinearity on the waveform is avoided by adding a standby between the subpulses. Theoretical analysis is validated by practical experiments based on a C-band SAR system.
Controlled electron injection into laser wakefields with a perpendicular injection laser pulse
Wang, W.-M.; Sheng, Z.-M.; Zhang, J.
2008-11-17
Electron injection into laser wakefields for acceleration by two orthogonally directed laser pulses is investigated theoretically. It is found that efficient injection occurs provided the two pulses are collinearly polarized, even if the injection pulse is much weaker than the pump pulse driving wakefields. Compared with the head-on colliding injection geometry, this scheme allows for a shorter propagation distance less than a Rayleigh length for the injection pulse, before its overlapping with the pump pulse. Moreover, it can generate electron beams stably with comparable low energy spread and emittance, as demonstrated by particle-in-cell simulations. The optimization of laser parameters is also investigated.
Applications of MALDI-TOF MS in environmental microbiology.
Santos, Inês C; Hildenbrand, Zacariah L; Schug, Kevin A
2016-05-10
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is an emerging technique for microbial identification, characterization, and typing. The single colony method can be used for obtaining a protein fingerprint or profile unique to each microorganism. This technique has been mainly used in the clinical field, but it also has significant potential in the environmental field. The applications of MALDI-TOF MS in environmental microbiology are discussed in this review. An overview on the use of MALDI-TOF MS for environmental proteomics and metabolomics is given as well as its use for bacterial strain typing and bioremediation research. A more detailed review on the use of this technique for the identification, differentiation, and categorization of environmental microorganisms is given. Some of the parameters that can influence the results and reproducibility of MALDI-TOF MS are also discussed. PMID:27072574
Moran, M J; Bond, E J; Clancy, T J; Eckart, M J; Khater, H Y; Glebov, V Y
2012-05-07
The first several campaigns of laser fusion experiments at the National Ignition Facility (NIF) included a family of high-sensitivity scintillator/photodetector neutron-time-of-flight (nTOF) detectors for measuring DD and DT neutron yields. The detectors provided consistent neutron yield benchmarks from below 1E9 (DD) to nearly 1E15 (DT). The detectors demonstrated DT yield measurement precisions better than 5%, but the absolute accuracy relies on cross calibration with independent measurements of absolute neutron yield. The 4.5-m nTOF data have provided a useful testbed for testing improvements in nTOF data processing, especially with respect to improving the accuracies of the detector impulse response functions. The resulting improvements in data analysis methods have produced more accurate results. In summary, results from the NIF 4.5-m nTOF detectors have provided consistent measurements of DD and DT neutron yields from laser-fusion implosions.
Lewin, Mark; Guilhaus, Michael; Wildgoose, Jason; Hoyes, John; Bateman, Bob
2002-01-01
Ions experience small deflections in the vicinity of grids in accelerators and ion mirrors in time-of-flight (TOF) mass spectrometers. Recent experiments with an orthogonal acceleration (oa) TOF instrument have verified that the effect can significantly degrade resolution when ions approach grids at an angle deviating from 90 degrees. The phenomenon becomes significant only when ions have components of velocity at right angles to the wires of the grids. A model is presented in this study to predict this phenomenon for parallel wire grids. The fractional energy spread of ions (calculated in the static TOF-spectrometer frame of reference) scales directly with the approach angle of ions to the grid (as measured from normal approach). The energy spread also scales with the range of angles that is a consequence of the focusing effect in each gap between the wires of the grid. The equations imply that closely spaced parallel wire grids are best for deployment in oa-TOF systems where non-zero approach angles are unavoidable. Such grids are relatively impractical to manufacture and support but rectangular repeat cell grids with relatively few wires at right angles to the source axis are shown experimentally to introduce minimal energy spread. When these grids are rotated by 90 degrees, the resolution measured in a Q-TOF spectrometer is degraded in approximate agreement with the parallel wire model. A practical implication of this work is that grid transmissions in oa-TOF systems may be significantly increased without loss of resolution. Improvements of approximately 200% (V-mode) and approximately 400% (W-mode) in ion transmission were obtained in this study without compromising resolution. This was achieved with approximately 73% transmission grids and greater potential improvements in transmission are being realised since this study with approximately 89% transmission grids having similar geometry. PMID:11870899
Orthogonal photoswitching in a multifunctional molecular system
Lerch, Michael M.; Hansen, Mickel J.; Velema, Willem A.; Szymanski, Wiktor; Feringa, Ben L.
2016-01-01
The wavelength-selective, reversible photocontrol over various molecular processes in parallel remains an unsolved challenge. Overlapping ultraviolet-visible spectra of frequently employed photoswitches have prevented the development of orthogonally responsive systems, analogous to those that rely on wavelength-selective cleavage of photo-removable protecting groups. Here we report the orthogonal and reversible control of two distinct types of photoswitches in one solution, that is, a donor–acceptor Stenhouse adduct (DASA) and an azobenzene. The control is achieved by using three different wavelengths of irradiation and a thermal relaxation process. The reported combination tolerates a broad variety of differently substituted photoswitches. The presented system is also extended to an intramolecular combination of photoresponsive units. A model application for an intramolecular combination of switches is presented, in which the DASA component acts as a phase-transfer tag, while the azobenzene moiety independently controls the binding to α-cyclodextrin. PMID:27401266
Describing freeform surfaces with orthogonal functions
NASA Astrophysics Data System (ADS)
Ochse, D.; Uhlendorf, K.; Reichmann, L.
2015-09-01
In optical design with freeform surfaces descriptions of the surfaces are needed that use only few parameters and are suitable for optimisation. Depending on the merit function - spot size or wavefront error - and the position of the surface in the system, different surface types can yield different optimisation performance. It has been demonstrated by G. Forbes that slope orthogonal polynomials are an advantageous freeform description. From literature on Gaussian moments it is known that this can be achieved using differences of Zernike polynomials, which are easy to compute and implement with recent algorithms. We will demonstrate the benefits of Zernike polynomials with optimisation examples. Furthermore we present an orthogonal surface representation on a rectangular aperture based on Chebyshev polynomials. This description is very convenient when the aperture has a very high aspect ratio, or when designing a system with a rectangular pupil.
HOLA: Human-like Orthogonal Network Layout.
Kieffer, Steve; Dwyer, Tim; Marriott, Kim; Wybrow, Michael
2016-01-01
Over the last 50 years a wide variety of automatic network layout algorithms have been developed. Some are fast heuristic techniques suitable for networks with hundreds of thousands of nodes while others are multi-stage frameworks for higher-quality layout of smaller networks. However, despite decades of research currently no algorithm produces layout of comparable quality to that of a human. We give a new "human-centred" methodology for automatic network layout algorithm design that is intended to overcome this deficiency. User studies are first used to identify the aesthetic criteria algorithms should encode, then an algorithm is developed that is informed by these criteria and finally, a follow-up study evaluates the algorithm output. We have used this new methodology to develop an automatic orthogonal network layout method, HOLA, that achieves measurably better (by user study) layout than the best available orthogonal layout algorithm and which produces layouts of comparable quality to those produced by hand. PMID:26390483
Orthogonal gradient networks via post polymerization reaction
NASA Astrophysics Data System (ADS)
Chinnayan Kannan, Pandiyarajan; Genzer, Jan
2015-03-01
We report a novel synthetic route to generate orthogonal gradient networks through post polymerization reaction using pentaflurophenylmethacrylate (PFPMAc) active ester chemistry. These chemoselective monomers were successfully copolymerized with 5 mole% of the photo (methacryloyloxybenzophenone) and thermal (styrenesulfonylazide) crosslinkers. Subsequently, the copolymers were modified by a series of amines having various alkyl chain lengths. The conversion of post polymerization reaction was monitored using Fourier Transform Infrared Spectroscopy (FT-IR) and noticed that almost all pentaflurophenyl moieties are substituted by amines within in an hour without affecting the crosslinkers. In addition, the incorporation of photo and thermal crosslinkers in the polymer enabled us to achieve stable and covalently surface-bound polymer gradient networks (PGN) in an orthogonal manner, i.e. complete control over the crosslink density of the network in two opposite directions (i.e. heat vs photo). The network properties such as wettability, swelling and tensile modulus of the gradient coatings are studied and revealed in the paper.
Orthogonalized operators for the f shell
Judd, B.R.; Crosswhite, H.
1984-04-01
Orthogonalized operators are introduced in the atomic configurations f/sup N/ in order to yield parameters that are more precisely defined and more stable than the conventional ones. Of the four Racah operators e/sub 0/, e/sub 1/, e/sub 2/, only e/sub 1/ needs adjusting. The set of two-electron scalars is made complete by the generalized Trees operators e/sub ..cap alpha../', e/sub ..beta../', and e/sub ..gamma../'. Of the three-electron scalars t/sub i/, only t/sub 2/ requires alteration. The theory is illustrated for f/sup 3/ by adding the orthogonalized operators in successive steps and comparing the fits with those obtained if the conventional operators are used.
MALDI-TOF MS quantification of coccidiostats in poultry feeds.
Wang, J; Sporns, P
2000-07-01
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a relatively new technique that is having a great impact on analyses. This study is the first to demonstrate the use of linear MALDI-TOF MS to identify and quantify coccidiostats in poultry feeds. 2,5-Dihydroxybenzoic acid (DHB) was found to be the best matrix. In MALDI-TOF MS, coccidiostats form predominantly [M + Na](+) ions, with additional small amounts of [M + K](+) and [M - H + 2Na](+) ions, and no obvious fragment ions. Salinomycin and narasin were unstable in the concentrated DHB matrix solution but were stable when dried on the MALDI-TOF MS probe. A simple fast Sep-pak C18 cartridge purification procedure was developed for the MALDI-TOF MS quantification of coccidiostats in poultry feeds. The MALDI-TOF MS limit of detection for lasalocid, monensin, salinomycin, and narasin standards was 251, 22, 24, and 24 fmol, respectively. The method detection limit for salinomycin and narasin in poultry feeds was 2.4 microgram/g. PMID:10898626
Orthogonal nilpotent superfields from linear models
NASA Astrophysics Data System (ADS)
Kallosh, Renata; Karlsson, Anna; Mosk, Benjamin; Murli, Divyanshu
2016-05-01
We derive supersymmetry/supergravity models with constrained orthogonal nilpotent superfields from the linear models in the formal limit where the masses of the sgoldstino, inflatino and sinflaton tend to infinity. The case where the sinflaton mass remains finite leads to a model with a `relaxed' constraint, where the sinflaton remains an independent field. Our procedure is equivalent to a requirement that some of the components of the curvature of the moduli space tend to infinity.
Stochastic processes with orthogonal polynomial eigenfunctions
NASA Astrophysics Data System (ADS)
Griffiths, Bob
2009-12-01
Markov processes which are reversible with either Gamma, Normal, Poisson or Negative Binomial stationary distributions in the Meixner class and have orthogonal polynomial eigenfunctions are characterized as being processes subordinated to well-known diffusion processes for the Gamma and Normal, and birth and death processes for the Poisson and Negative Binomial. A characterization of Markov processes with Beta stationary distributions and Jacobi polynomial eigenvalues is also discussed.
Orthogonal rational functions and quadrature on an interval
NASA Astrophysics Data System (ADS)
van Deun, J.; Bultheel, A.
2003-04-01
Rational functions with real poles and poles in the complex lower half-plane, orthogonal on the real line, are well known. Quadrature formulas similar to the Gauss formulas for orthogonal polynomials have been studied. We generalize to the case of arbitrary complex poles and study orthogonality on a finite interval. The zeros of the orthogonal rational functions are shown to satisfy a quadratic eigenvalue problem. In the case of real poles, these zeros are used as nodes in the quadrature formulas.
Multicarrier orthogonal spread-spectrum (MOSS) data communications
Smith, Stephen F.; Dress, William B.
2008-01-01
Systems and methods are described for multicarrier orthogonal spread-spectrum (MOSS) data communication. A method includes individually spread-spectrum modulating at least two of a set of orthogonal frequency division multiplexed carriers, wherein the resulting individually spread-spectrum modulated at least two of a set of orthogonal frequency division multiplexed carriers are substantially mutually orthogonal with respect to both frequency division multiplexing and spread-spectrum modulation.
Nested Krylov methods and preserving the orthogonality
NASA Technical Reports Server (NTRS)
Desturler, Eric; Fokkema, Diederik R.
1993-01-01
Recently the GMRESR inner-outer iteraction scheme for the solution of linear systems of equations was proposed by Van der Vorst and Vuik. Similar methods have been proposed by Axelsson and Vassilevski and Saad (FGMRES). The outer iteration is GCR, which minimizes the residual over a given set of direction vectors. The inner iteration is GMRES, which at each step computes a new direction vector by approximately solving the residual equation. However, the optimality of the approximation over the space of outer search directions is ignored in the inner GMRES iteration. This leads to suboptimal corrections to the solution in the outer iteration, as components of the outer iteration directions may reenter in the inner iteration process. Therefore we propose to preserve the orthogonality relations of GCR in the inner GMRES iteration. This gives optimal corrections; however, it involves working with a singular, non-symmetric operator. We will discuss some important properties, and we will show by experiments that, in terms of matrix vector products, this modification (almost) always leads to better convergence. However, because we do more orthogonalizations, it does not always give an improved performance in CPU-time. Furthermore, we will discuss efficient implementations as well as the truncation possibilities of the outer GCR process. The experimental results indicate that for such methods it is advantageous to preserve the orthogonality in the inner iteration. Of course we can also use iteration schemes other than GMRES as the inner method; methods with short recurrences like GICGSTAB are of interest.
Functional systems with orthogonal dynamic covalent bonds.
Wilson, Adam; Gasparini, Giulio; Matile, Stefan
2014-03-21
This review summarizes the use of orthogonal dynamic covalent bonds to build functional systems. Dynamic covalent bonds are unique because of their dual nature. They can be as labile as non-covalent interactions or as permanent as covalent bonds, depending on conditions. Examples from nature, reaching from the role of disulfides in protein folding to thioester exchange in polyketide biosynthesis, indicate how dynamic covalent bonds are best used in functional systems. Several synthetic functional systems that employ a single type of dynamic covalent bonds have been reported. Considering that most functional systems make simultaneous use of several types of non-covalent interactions together, one would expect the literature to contain many examples in which different types of dynamic covalent bonds are similarly used in tandem. However, the incorporation of orthogonal dynamic covalent bonds into functional systems is a surprisingly rare and recent development. This review summarizes the available material comprehensively, covering a remarkably diverse collection of functions. However, probably more revealing than the specific functions addressed is that the questions asked are consistently quite unusual, very demanding and highly original, focusing on molecular systems that can self-sort, self-heal, adapt, exchange, replicate, transcribe, or even walk and "think" (logic gates). This focus on adventurous chemistry off the beaten track supports the promise that with orthogonal dynamic covalent bonds we can ask questions that otherwise cannot be asked. The broad range of functions and concepts covered should appeal to the supramolecular organic chemist but also to the broader community. PMID:24287608
Orthogonal NGS for High Throughput Clinical Diagnostics
Chennagiri, Niru; White, Eric J.; Frieden, Alexander; Lopez, Edgardo; Lieber, Daniel S.; Nikiforov, Anastasia; Ross, Tristen; Batorsky, Rebecca; Hansen, Sherry; Lip, Va; Luquette, Lovelace J.; Mauceli, Evan; Margulies, David; Milos, Patrice M.; Napolitano, Nichole; Nizzari, Marcia M.; Yu, Timothy; Thompson, John F.
2016-01-01
Next generation sequencing is a transformative technology for discovering and diagnosing genetic disorders. However, high-throughput sequencing remains error-prone, necessitating variant confirmation in order to meet the exacting demands of clinical diagnostic sequencing. To address this, we devised an orthogonal, dual platform approach employing complementary target capture and sequencing chemistries to improve speed and accuracy of variant calls at a genomic scale. We combined DNA selection by bait-based hybridization followed by Illumina NextSeq reversible terminator sequencing with DNA selection by amplification followed by Ion Proton semiconductor sequencing. This approach yields genomic scale orthogonal confirmation of ~95% of exome variants. Overall variant sensitivity improves as each method covers thousands of coding exons missed by the other. We conclude that orthogonal NGS offers improvements in variant calling sensitivity when two platforms are used, better specificity for variants identified on both platforms, and greatly reduces the time and expense of Sanger follow-up, thus enabling physicians to act on genomic results more quickly. PMID:27090146
Constrained predictive control using orthogonal expansions
Finn, C.K. ); Wahlberg, B. . Dept. of Automatic Control); Ydstie, B.E. . Dept. of Chemical Engineering)
1993-11-01
Orthogonal expansion is routinely used for multivariable predictive control and optimization in the chemical and petrochemical manufacturing industries. In this article, the authors approximate bounded operators by orthogonal expansion. The rate of convergence depends on the choice of basis functions. Markov-Laguerre functions give rapid convergence for open-loop stable systems with long delay. The Markov-Kautz model can be used for lightly damped systems, and a more general orthogonal expansion is developed for modeling multivariable systems with widely scattered poles. The finite impulse response model is a special case of these models. A-priori knowledge about dominant time constants, time delay and oscillatory modes is used to reduce the model complexity and to improve conditioning of the parameter estimation algorithm. Algorithms for predictive control are developed, as well as conditions for constraint compatibility, closed-loop stability and constraint satisfaction for the ideal case. An H[infinity]--like design technique proposed guarantees robust stability in the presence of input constraints; output constraints may give chatter. A chatter-free algorithm is proposed.
2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing.
Petry-Podgórska, Inga; Zídková, Jitka; Flodrová, Dana; Bobálová, Janette
2010-11-15
The barley proteins have been the subject of interests of many research groups dealing with barley grains, malt and beer. The proteins which remain intact after harsh malting conditions influence the quality and flavor of beer. The characteristic feature of the proteins present in malt and beer is their extensive modification with carbohydrates, mainly glucose that comes from the starch degradation during technological processes. The degree of the protein glycation has an effect on the quality of malt and beer and on the properties of the beer foam. A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called "shot-gun" approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting. PMID:20956095
NASA Astrophysics Data System (ADS)
Yan, Yuetian; Ubukata, Masaaki; Cody, Robert B.; Holy, Timothy E.; Gross, Michael L.
2014-08-01
A method for structural elucidation of biomolecules dating to the 1980s utilized high-energy collisions (~10 keV, laboratory frame) that induced charge-remote fragmentations (CRF), a class of fragmentations particularly informative for lipids, steroids, surfactants, and peptides. Unfortunately, the capability for high-energy activation has largely disappeared with the demise of magnetic sector instruments. With the latest designs of tandem time-of-flight mass spectrometers (TOF/TOF), however, this capability is now being restored to coincide with the renewed interest in metabolites and lipids, including steroid-sulfates and other steroid metabolites. For these metabolites, structure determinations are required at concentration levels below that appropriate for NMR. To meet this need, we explored CRF with TOF/TOF mass spectrometry for two groups of steroid sulfates, 3-sulfates and 21-sulfates. We demonstrated that the current generation of MALDI TOF/TOF instruments can generate charge-remote fragmentations for these materials. The resulting collision-induced dissociation (CID) spectra are useful for positional isomer differentiation and very often allow the complete structure determination of the steroid. We also propose a new nomenclature that directly indicates the cleavage sites on the steroid ring with carbon numbers.
Koehbach, Johannes; Gruber, Christian W; Becker, Christian; Kreil, David P; Jilek, Alexander
2016-05-01
Several biologically active peptides contain a d- amino acid in a well-defined position, which is position 2 in all peptide epimers isolated to date from vertebrates and also some from invertebrates. The detection of such D- residues by standard analytical techniques is challenging. In tandem mass spectrometric (MS) analysis, although fragment masses are the same for all stereoisomers, peak intensities are known to depend on chirality. Here, we observe that the effect of a d- amino acid in the second N-terminal position on the fragmentation pattern in matrix assisted laser desorption time-of-flight spectrometry (MALDI-TOF/TOF MS) strongly depends on the peptide sequence. Stereosensitive fragmentation (SF) is correlated to a neighborhood effect, but the d- residue also exerts an overall effect influencing distant bonds. In a fingerprint analysis, multiple peaks can thus serve to identify the chirality of a sample in short time and potentially high throughput. Problematic variations between individual spots could be successfully suppressed by cospotting deuterated analogues of the epimers. By identifying the [d-Leu2] isomer of the predicted peptide GH-2 (gene derived bombininH) in skin secretions of the toad Bombina orientalis, we demonstrated the analytical power of SF-MALDI-TOF/TOF measurements. In conclusion, SF-MALDI-TOF/TOF MS combines high sensitivity, versatility, and the ability to complement other methods. PMID:26985971
2016-01-01
Several biologically active peptides contain a d- amino acid in a well-defined position, which is position 2 in all peptide epimers isolated to date from vertebrates and also some from invertebrates. The detection of such D- residues by standard analytical techniques is challenging. In tandem mass spectrometric (MS) analysis, although fragment masses are the same for all stereoisomers, peak intensities are known to depend on chirality. Here, we observe that the effect of a d- amino acid in the second N-terminal position on the fragmentation pattern in matrix assisted laser desorption time-of-flight spectrometry (MALDI-TOF/TOF MS) strongly depends on the peptide sequence. Stereosensitive fragmentation (SF) is correlated to a neighborhood effect, but the d- residue also exerts an overall effect influencing distant bonds. In a fingerprint analysis, multiple peaks can thus serve to identify the chirality of a sample in short time and potentially high throughput. Problematic variations between individual spots could be successfully suppressed by cospotting deuterated analogues of the epimers. By identifying the [d-Leu2] isomer of the predicted peptide GH-2 (gene derived bombininH) in skin secretions of the toad Bombina orientalis, we demonstrated the analytical power of SF-MALDI-TOF/TOF measurements. In conclusion, SF-MALDI-TOF/TOF MS combines high sensitivity, versatility, and the ability to complement other methods. PMID:26985971
... with other medications. Paclitaxel injection manufactured with polyoxyethylated castor oil is used to treat ovarian cancer (cancer that ... cancer, and lung cancer. Paclitaxel injection with polyoxyethylated castor oil is also used to treat Kaposi's sarcoma (a ...
... become pregnant during your treatment, stop using mipomersen injection and call your doctor immediately. ... Mipomersen injection may cause side effects. Tell your doctor if any of these ... and tiredness that are most likely to occur during the first 2 days ...
... infections. Levofloxacin injection is also used to prevent anthrax (a serious infection that may be spread on ... in people who may have been exposed to anthrax germs in the air. Levofloxacin injection is in ...
... injection is also used to prevent or treat anthrax (a serious infection that may be spread on ... in people who may have been exposed to anthrax germs in the air. Ciprofloxacin injection is in ...
... with at least one other medication given by mouth or by injection. Romidepsin injection is in a ... antifungals such as itraconazole (Sporanox), ketoconazole (Nizoral), and voriconazole (Vfend); cisapride (Propulsid) (not available in the U.S.); ...
Degarelix injection is used to treat advanced prostate cancer (cancer that begins in the prostate [a male reproductive gland]). Degarelix injection is in a class of medications called gonadotropin-releasing hormone (GnRH) ...
... other medications. Paclitaxel injection manufactured with polyoxyethylated castor oil is used to treat ovarian cancer (cancer that ... and lung cancer. Paclitaxel injection with polyoxyethylated castor oil is also used to treat Kaposi's sarcoma (a ...
... course of disease where symptoms flare up from time to time) of multiple sclerosis (MS; a disease in which ... to inject glatiramer, inject it around the same time every day. Follow the directions on your prescription ...
... any laboratory test, tell your doctor and the laboratory personnel that you are receiving or received daratumumab injection. ... a blood transfusion, tell your doctor and the laboratory personnel that you are receiving or received daratumumab injection. ...
... will need to take folic acid and vitamin B12 during your treatment with pralatrexate injection to help ... that you will need to receive a vitamin B12 injection no more than 10 weeks before your ...
... injection is used to treat infections caused by bacteria including pneumonia and other lower respiratory tract (lung) ... medications called cephamycin antibiotics. It works by killing bacteria.Antibiotics such as cefoxitin injection will not work ...
... treat certain types of serious infections caused by bacteria when other antibiotics cannot be used. Chloramphenicol injection ... antibiotics. It works by stopping the growth of bacteria..Antibiotics such as chloramphenicol injection will not work ...
... is used to treat infections caused by certain bacteria. Oxacillin injection is in a class of medications called penicillins. It works by killing bacteria.Antibiotics such as oxacillin injection will not work ...
... to treat infections caused by certain types of bacteria. Nafcillin injection is in a class of medications called penicillins. It works by killing bacteria.Antibiotics such as nafcillin injection will not work ...
... tract, kidney, and abdomen that are caused by bacteria. Doripenem injection is not approved by the Food ... medications called carbapenem antibiotics. It works by killing bacteria.Antibiotics such as doripenem injection will not work ...
... Medroxyprogesterone injection is a very effective method of birth control but does not prevent the spread of human ... you have been using a different method of birth control and are switching to medroxyprogesterone injection, your doctor ...
Chloramphenicol injection is used to treat certain types of serious infections caused by bacteria when other antibiotics cannot be used. Chloramphenicol injection is in a class of medications called ...
... injection is used to prevent harmful effects of methotrexate (Rheumatrex, Trexall) when methotrexate is used to to treat certain types of ... people who have accidentally received an overdose of methotrexate or similar medications. Levoleucovorin injection is in a ...
... forms of estrogen injection are used to treat hot flushes (hot flashes; sudden strong feelings of heat and sweating) ... If you are using estrogen injection to treat hot flushes, your symptoms should improve within 1 to ...
Palonosetron injection is used to prevent nausea and vomiting that may occur within 24 hours after receiving ... occur several days after receiving certain chemotherapy medications. Palonosetron injection is in a class of medications called ...
... normal number of red blood cells) caused by uterine fibroids (noncancerous growths in the uterus). Leuprolide injection is ... Your doctor will tell you how long your treatment with leuprolide injection will last. When used in ...
Naltrexone injection is used along with counseling and social support to help people who have stopped drinking large ... injection is also used along with counseling and social support to help people who have stopped abusing opiate ...
Posaconazole injection is used to prevent fungal infections in people with a weakened ability to fight infection. Posaconazole injection is in a class of medications called azole antifungals. It works ...
Adrenalin® Chloride Solution ... a pre-filled automatic injection device containing a solution (liquid) to inject under the skin or into ... device when this date passes. Look at the solution in the device from time to time. If ...
Trastuzumab injection is used along with other medications or after other medications have been used to treat ... has spread to other parts of the body. Trastuzumab injection is also used during and after treatment ...
... using fondaparinux injection while you are in the hospital at least 6 to 8 hours after your ... you will continue to use fondaparinux after your hospital stay, you can inject fondaparinux yourself or have ...
Doxycycline injection is used to treat or prevent bacterial infections, including pneumonia and other respiratory tract infections. ... certain skin, genital, intestine, and urinary system infections. Doxycycline injection may be used to treat or prevent ...
... Medroxyprogesterone subcutaneous injection is also used to treat endometriosis (a condition in which the type of tissue ... parts of the body in women who have endometriosis. Medroxyprogesterone injection is a very effective method of ...
Ferumoxytol injection is used to treat iron-deficiency anemia (a lower than normal number of red blood ... and may cause the kidneys to stop working). Ferumoxytol injection is in a class of medications called ...
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Momentum space orthogonal polynomial projection quantization
NASA Astrophysics Data System (ADS)
Handy, C. R.; Vrinceanu, D.; Marth, C. B.; Gupta, R.
2016-04-01
The orthogonal polynomial projection quantization (OPPQ) is an algebraic method for solving Schrödinger’s equation by representing the wave function as an expansion {{\\Psi }}(x)={\\displaystyle \\sum }n{{{Ω }}}n{P}n(x)R(x) in terms of polynomials {P}n(x) orthogonal with respect to a suitable reference function R(x), which decays asymptotically not faster than the bound state wave function. The expansion coefficients {{{Ω }}}n are obtained as linear combinations of power moments {μ }{{p}}=\\int {x}p{{\\Psi }}(x) {{d}}x. In turn, the {μ }{{p}}'s are generated by a linear recursion relation derived from Schrödinger’s equation from an initial set of low order moments. It can be readily argued that for square integrable wave functions representing physical states {{lim}}n\\to ∞ {{{Ω }}}n=0. Rapidly converging discrete energies are obtained by setting Ω coefficients to zero at arbitrarily high order. This paper introduces an extention of OPPQ in momentum space by using the representation {{Φ }}(k)={\\displaystyle \\sum }n{{{\\Xi }}}n{Q}n(k)T(k), where Q n (k) are polynomials orthogonal with respect to a suitable reference function T(k). The advantage of this new representation is that it can help solving problems for which there is no coordinate space moment equation. This is because the power moments in momentum space are the Taylor expansion coefficients, which are recursively calculated via Schrödinger’s equation. We show the convergence of this new method for the sextic anharmonic oscillator and an algebraic treatment of Gross-Pitaevskii nonlinear equation.
Heisenberg algebra, umbral calculus and orthogonal polynomials
Dattoli, G.; Levi, D.; Winternitz, P.
2008-05-15
Umbral calculus can be viewed as an abstract theory of the Heisenberg commutation relation [P,M]=1. In ordinary quantum mechanics, P is the derivative and M the coordinate operator. Here, we shall realize P as a second order differential operator and M as a first order integral one. We show that this makes it possible to solve large classes of differential and integrodifferential equations and to introduce new classes of orthogonal polynomials, related to Laguerre polynomials. These polynomials are particularly well suited for describing the so-called flatenned beams in laser theory.
Observations on the Proper Orthogonal Decomposition
NASA Technical Reports Server (NTRS)
Berkooz, Gal
1992-01-01
The Proper Orthogonal Decomposition (P.O.D.), also known as the Karhunen-Loeve expansion, is a procedure for decomposing a stochastic field in an L(2) optimal sense. It is used in diverse disciplines from image processing to turbulence. Recently the P.O.D. is receiving much attention as a tool for studying dynamics of systems in infinite dimensional space. This paper reviews the mathematical fundamentals of this theory. Also included are results on the span of the eigenfunction basis, a geometric corollary due to Chebyshev's inequality and a relation between the P.O.D. symmetry and ergodicity.
Analysis of chromatograph systems using orthogonal collocation
NASA Technical Reports Server (NTRS)
Woodrow, P. T.
1974-01-01
Research is generating fundamental engineering design techniques and concepts for the chromatographic separator of a chemical analysis system for an unmanned, Martian roving vehicle. A chromatograph model is developed which incorporates previously neglected transport mechanisms. The numerical technique of orthogonal collocation is studied. To establish the utility of the method, three models of increasing complexity are considered, the latter two being limiting cases of the derived model: (1) a simple, diffusion-convection model; (2) a rate of adsorption limited, inter-intraparticle model; and (3) an inter-intraparticle model with negligible mass transfer resistance.
Noncommutative Pfaffians associated with the orthogonal algebra
Artamonov, Dmitrii V; Golubeva, Valentina A
2012-12-31
Commutators of Pfaffians associated with the orthogonal algebra are found in skew-symmetric and root realizations of o{sub N}. A generating function of Pfaffians is proved to satisfy the reflection equation. A relation between Pfaffians in skew-symmetric and root realizations of o{sub N} is established. Using these results we construct an integrable equation of Knizhnik-Zamolodchikov type using the Capelli central elements in U(o{sub N}), which are sums of squares of the considered Pfaffians. A classical limit of the obtained Knizhnik-Zamolodchikov type equation turns out to be a very specific system of equations of isomonodromic deformations. Bibliography: 18 titles.
Llorach, Rafael; Medina, Sonia; García-Viguera, Cristina; Zafrilla, Pilar; Abellán, José; Jauregui, Olga; Tomás-Barberán, Francisco A; Gil-Izquierdo, Angel; Andrés-Lacueva, Cristina
2014-06-01
Metabolomics has emerged in the field of food and nutrition sciences as a powerful tool for doing profiling approaches. In this context, HPLC-q-TOF-based metabolomics approach was applied to unveil changes in the urinary metabolome in human subjects (n = 51, 23 men and 28 women) after regular aronia-citrus juice (AC-juice) intake (250 mL/day) during 16 weeks compared to individuals given a placebo beverage. Samples were analyzed by HPLC-q-TOF followed by multivariate data analysis (orthogonal signal filtering-partial least square discriminant analysis) that discriminated relevant mass features related to AC-juice intake. The results showed that biomarkers of AC-juice intake including metabolites coming from metabolism of food components as proline betaine, ferulic acid, and two unknown mercapturate derivatives were identified. Discovery of new biomarkers of food intake will help in the building up of the food metabolome and facilitate future insights into the mechanisms of action of dietary components in population health. PMID:24616048
Ji, Jian; Sun, Jiadi; Pi, Fuwei; Zhang, Shuang; Sun, Chao; Wang, Xiumei; Zhang, Yinzhi; Sun, Xiulan
2016-08-25
Gas chromatography-time of fly/mass spectrum (GC-TOF/MS) based complete murine macrophage ANA-1 cell metabolome strategy, including the endo-metabolome and the exo-metabolome, ANA-1 cell viability assays and apoptosis induced by diverse concentrations of DON were evaluated for selection of an optimized dose for in-depth metabolomic research. Using the optimized chromatography and mass spectrometry parameters, the metabolites detected by GC-TOF/MS were identified and processed with multivariate statistical analysis, including principal componentanalysis (PCA) and orthogonal projection to latent structures-discriminant analysis (OPLS-DA) analysis. The data sets were screened with a t-test (P) value < 0.05, VIP value > 1, similarity value > 500, leaving 16 exo-metabolite variables and 11 endo-metabolite variables for further pathway analysis. Implementing the integration of key metabolic pathways, the metabolism pathways were categorized into two dominating types, metabolism of amino acid and glycometabolism. Glycine, serine and threonine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis and phenylalanine metabolism were the significant amino acids affected by the metabolic pathways, indicating statistically significant fold changes including pyruvate, serine, glycine, lactate and threonine. Glycolysis or gluconeogenesis, starch and sucrose metabolism, and galactose metabolism, belonging to glycometabolism, were the pathways that were found to be primarily affected, resulting in abnormal metabolites such as glucose-1P, Glucose, gluconic acid, myo-inositol, sorbitol and glycerol. PMID:27350164
Geometric reconstruction of biological orthogonal plywoods.
Aguilar Gutierrez, Oscar F; Rey, Alejandro D
2016-01-28
In this paper we focus on the structural determination of biological orthogonal plywoods, fiber-like composite analogues of liquid crystalline phases, where the fibrils of the building blocks show sharp 90° orientation jumps between fibers in adjacent domains. We present an original geometric and computational modelling that allows us to determine the fibrillary orientation in biological plywoods from periodic herringbone patterns commonly observed in cross-sections. Although herringbone patterns were long reported, the specific and quantitative relationships between herringbones and the orthogonal plywoods were absent or at best incomplete. Here we provide an efficient and new procedure to perform an inverse problem that connects two specific features of the herringbone patterns (aperture angle and wavelength) with the 3D morphology of the structure, whose accuracy and validity were ascertained through in silico simulations and also with real specimens ("Eremosphaera viridis"). This contribution extends significantly the better known characterization methods of 2D cross sections, such as the arced patterns observed in biological helicoidal plywoods, and with the present proposed methodology it adds another characterization tool for a variety of biological fibrous composites that form cornea-like tissues. PMID:26583687
TOF-SIMS imaging of lipids on rat brain sections.
Touboul, David; Brunelle, Alain
2015-01-01
Since several decades, secondary ion mass spectrometry (SIMS) coupled to time of flight (TOF) is used for atomic or small inorganic/organic fragments imaging on different materials. With the advent of polyatomic ion sources leading to a significant increase of sensitivity in combination with a reasonable spatial resolution (1-10 μm), TOF-SIMS is becoming a more and more popular analytical platform for MS imaging. Even if this technique is limited to small molecules (typically below 1,000 Da), it offers enough sensitivity to detect and locate various classes of lipids directly on the surface of tissue sections. This chapter is thus dedicated to the TOF-SIMS analysis of lipids in positive and negative ion modes on rat brain tissue sections using a bismuth cluster ion source. PMID:25361663
Identification of flea species using MALDI-TOF/MS.
Yssouf, Amina; Socolovschi, Cristina; Leulmi, Hamza; Kernif, Tahar; Bitam, Idir; Audoly, Gilles; Almeras, Lionel; Raoult, Didier; Parola, Philippe
2014-05-01
In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas. PMID:24878069
Speciation of surface gold in pressure oxidized carbonaceous gold ores by TOF-SIMS and TOF-LIMS
NASA Astrophysics Data System (ADS)
Dimov, S. S.; Chryssoulis, S. L.; Sodhi, R. N.
2003-01-01
To the best of our knowledge, this is the first attempt ever to speciate gold preg-robbed by carbonaceous matter using a surface sensitive microbeam technique. This approach enables the direct determination of gold species sorbed on carbonaceous particulates thus providing a new tool in understanding the chemistry of gold sorption on carbon. The reasoning behind this effort was to study the detrimental effect chloride ions have on gold recovery by pressure oxidation of carbonaceous sulfide ores, a technology largely used by the mining industry. The characterization of the sorbed gold species involved three surface sensitive microbeam analytical techniques (TOF-SIMS, TOF-LIMS and XPS) providing confirmatory results for better accuracy. Optimum conditions for detection of gold compounds with minimum fragmentation by TOF-SIMS and TOF-LIMS mass spectrometers have been determined. A reference library of 16 major gold complexes with halogen, thiosulfate, cyanide and thiocyanate groups relevant to the gold recovery processes has been established. The most suitable of the microbeam techniques tested was found to be negative (-ve) ion TOF-LIMS, offering best sensitivity and a small analytical spot size.
Fission of Actinides Induced by Neutrons at nTOF
Tassan-Got, L.; Audouin, L.; Berthier, B.; Ferrant, L.; Isaev, S.; Le Naour, C.; Stephan, C.; Trubert, D.; Duran, I.; Paradela, C.; Moreau, C.
2005-05-24
The neutron-induced fission cross sections of 233U, 234U, 232Th, 237Np, 209Bi, natPb have been measured on the nTOF facility at CERN, which allows an accurate energy measurement owing to the long path. Parallel plate avalanche counters were used to detect the 2 fission fragments in coincidence. This method allows an efficient discrimination of fission reactions among other types of reactions especially at high energies, and it is well suited for the very large energy range available at nTOF. The case of 234U will be used as an example of the quality of the data obtained in these measurements.
Qualitative and quantitative analyses of alkaloids in Uncaria species by UPLC-ESI-Q-TOF/MS.
Wang, Hai-Bo; Qi, Wen; Zhang, Lin; Yuan, Dan
2014-01-01
An ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (Q-TOF/MS) method has been optimized and established for the rapid analysis of the alkaloids in 22 samples originating from five Uncaria (U.) species. The accurate mass measurement of all the protonated molecules and subsequent fragment ions offers higher quality structural information for the interpretation of fragmentation pathways of the various groups of alkaloids. A total of 19 oxindole alkaloids, 16 indole alkaloids and 1 flavone were identified by co-chromatography of the sample extract with authentic standards, comparison of the retention time, characteristic molecular ions and fragment ions, or were tentatively identified by MS/MS determination. Moreover, the method was validated for the simultaneous quantification of the 24 components within 10.5 min. The potential chemical markers were identified for classification of the U. species samples by principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA). The results demonstrate the similarity and differences in alkaloids among the five U. species, which is helpful for the standardization and quality control of the medical materials of the U. Ramulus Cum Unics (URCU). Furthermore, with multivariate statistical analysis, the determined markers are more definite and useful for chemotaxonomy of the U. genus. PMID:25366313
How orthogonal are the OBO Foundry ontologies?
2011-01-01
Background Ontologies in biomedicine facilitate information integration, data exchange, search and query of biomedical data, and other critical knowledge-intensive tasks. The OBO Foundry is a collaborative effort to establish a set of principles for ontology development with the eventual goal of creating a set of interoperable reference ontologies in the domain of biomedicine. One of the key requirements to achieve this goal is to ensure that ontology developers reuse term definitions that others have already created rather than create their own definitions, thereby making the ontologies orthogonal. Methods We used a simple lexical algorithm to analyze the extent to which the set of OBO Foundry candidate ontologies identified from September 2009 to September 2010 conforms to this vision. Specifically, we analyzed (1) the level of explicit term reuse in this set of ontologies, (2) the level of overlap, where two ontologies define similar terms independently, and (3) how the levels of reuse and overlap changed during the course of this year. Results We found that 30% of the ontologies reuse terms from other Foundry candidates and 96% of the candidate ontologies contain terms that overlap with terms from the other ontologies. We found that while term reuse increased among the ontologies between September 2009 and September 2010, the level of overlap among the ontologies remained relatively constant. Additionally, we analyzed the six ontologies announced as OBO Foundry members on March 5, 2010, and identified that the level of overlap was extremely low, but, notably, so was the level of term reuse. Conclusions We have created a prototype web application that allows OBO Foundry ontology developers to see which classes from their ontologies overlap with classes from other ontologies in the OBO Foundry (http://obomap.bioontology.org). From our analysis, we conclude that while the OBO Foundry has made significant progress toward orthogonality during the period of this
Beta-integrals and finite orthogonal systems of Wilson polynomials
Neretin, Yu A
2002-08-31
The integral is calculated and the system of orthogonal polynomials with weight equal to the corresponding integrand is constructed. This weight decreases polynomially, therefore only finitely many of its moments converge. As a result the system of orthogonal polynomials is finite. Systems of orthogonal polynomials related to {sub 5}H{sub 5}-Dougall's formula and the Askey integral is also constructed. All the three systems consist of Wilson polynomials outside the domain of positiveness of the usual weight.
Orthogonal canonical forms for second-order systems
NASA Technical Reports Server (NTRS)
Williams, Trevor; Laub, Alan J.
1992-01-01
It is shown that a linear second-order system with arbitrary damping cannot be reduced to Hessenberg-triangular form by means of orthogonal transformations. However, it is also shown that such an orthogonal reduction is always possible for the modal damping commonly assumed for models of flexible structures. It is shown that modally damped models can be orthogonally reduced to a new triangular second-order Schur form.
Chemical Cross-linking and MALDI-TOF/TOF to Investigate Protein Interacting Domains
Pottiez, G.; Ciborowski, P.; Wojtkiewicz, Melinda
2012-01-01
INTRODUCTION: Mapping peptides modified by chemical cross-linker(s) provides clues about proteins' interacting domains. One complication is that such modification may result from intra- but not inter-molecular interactions. Western blot analyses showed an ability of pGSN to spontaneously form homo-dimers and homo-trimers, which we also detected in samples of human plasma. Therefore, main goal of this study was to use chemical cross-linking to map residues involved in inter- and/or intra-molecular interactions. METHODS: Two separate commercially available preparations were used in this study: recombinant expressed in E.coli (Cytoskeleton Inc.) and native purified from human plasma (Sigma, Inc.). Cross-linkers, Bis(sulfosuccinimidyl)glutarate-d0 (BS2G-d0), Bis(sulfo-succinimidyl)suberate-d0 (BS3G-d0), Bis-N-succinimidyl(PEG)5 (BS(PEG)5) and 3,3′-Dithiobis (sulfosuccinimidylpropionate) (DTSSP) were from Thermo Scientific (San Jose, CA, USA). For mass spectrometry analyses we used ABSciex 4800 MALDI-TOF/TOF. RESULTS: We used cross-linkers with arm length ranging from 7.7Å to 21.7Å. We present that MALDI based mass spectrometry generates high quality data to show lysine residues modified by cross-linkers and combined with existing data based on crystallography (Protein Data Bank, PDB) can be used to discriminate between inter- and intra-molecular linking. Interestingly, a 12.0Å linker did not support complete intra-molecular cross-linking of monomer like 11.4Å cross-linker resulting in the presence of two bands in 1-DE analysis. CONCLUSIONS: When interpreting results of in vitro cross-linking interacting proteins, a possibility of self cross-linking of any of interacting proteins should be considered. Therefore, critical residues for forming hetero-complexes can be blocked and new and not biologically relevant interacting domains can be created. Flexibility of some regions within the protein polypeptide chain may lead to linking of distant residues where linker
Multifunctional Surface Manipulation Using Orthogonal Click Chemistry.
Brooks, Karson; Yatvin, Jeremy; McNitt, Christopher D; Reese, R Alexander; Jung, Calvin; Popik, Vladimir V; Locklin, Jason
2016-07-01
Polymer brushes are excellent substrates for the covalent immobilization of a wide variety of molecules due to their unique physicochemical properties and high functional group density. By using reactive microcapillary printing, poly(pentafluorophenyl acrylate) brushes with rapid kinetic rates toward aminolysis can be partially patterned with other click functionalities such as strained cyclooctyne derivatives and sulfonyl fluorides. This trireactive surface can then react locally and selectively in a one pot reaction via three orthogonal chemistries at room temperature: activated ester aminolysis, strain promoted azide-alkyne cycloaddition, and sulfur(VI) fluoride exchange, all of which are tolerant of ambient moisture and oxygen. Furthermore, we demonstrate that these reactions can also be used to create areas of morphologically distinct surface features on the nanoscale, by inducing buckling instabilities in the films and the grafting of nanoparticles. This approach is modular, and allows for the development of highly complex surface motifs patterned with different chemistry and morphology. PMID:27280689
Confocal imaging with orthogonally polarized illumination beams
NASA Astrophysics Data System (ADS)
Kalita, Ranjan; Boruah, Bosanta R.
2016-03-01
In confocal microscopy the polarization of the illumination beam plays an important role in determining the orientation of the fluorescent molecules being illuminated. The efficiency of the excitation depends on the angle between the excitation electric field and the direction of the molecular dipole. In order to determine the orientation of the fluorescent molecules in the focal plane the molecules are to be excited using two mutually orthogonal electric fields. In this paper we show how a computer generated holography technique can be implemented using a ferroelectric liquid crystal spatial light modulator to conveniently obtain two images of the same target once with an X polarized illumination beam and another with a Y polarized illumination beam.
Response Surface Modeling Using Multivariate Orthogonal Functions
NASA Technical Reports Server (NTRS)
Morelli, Eugene A.; DeLoach, Richard
2001-01-01
A nonlinear modeling technique was used to characterize response surfaces for non-dimensional longitudinal aerodynamic force and moment coefficients, based on wind tunnel data from a commercial jet transport model. Data were collected using two experimental procedures - one based on modem design of experiments (MDOE), and one using a classical one factor at a time (OFAT) approach. The nonlinear modeling technique used multivariate orthogonal functions generated from the independent variable data as modeling functions in a least squares context to characterize the response surfaces. Model terms were selected automatically using a prediction error metric. Prediction error bounds computed from the modeling data alone were found to be- a good measure of actual prediction error for prediction points within the inference space. Root-mean-square model fit error and prediction error were less than 4 percent of the mean response value in all cases. Efficacy and prediction performance of the response surface models identified from both MDOE and OFAT experiments were investigated.
Description of sunspot cycles by orthogonal functions
NASA Astrophysics Data System (ADS)
Teuber, D. L.; Reichmann, E. J.; Wilson, R. M.
1984-10-01
Based on the principal component analysis technique and evidence for a 22-yr double-sunspot cycle periodicity. The time series of sunspot numbers is represented as a sum of mutually orthogonal eigenvectors in the time domain. It is shown that the first two eigenvectors account for about 90 percent of the cumulative 'signal power,' and that this is sufficient for reconstruction of the raw data curve. It is also noted that the second eigenvector behaves as the time derivative of the first, and that a phase-plane plot of these eigenvectors (i.e. a plot of a variable vs. its rate of change) suggests that the sun's sunspot cycle is driven by an oscillator; the implication is that, embedded within the sun, a chronometer is at work (e.g. Dicke, 1979).
Description of sunspot cycles by orthogonal functions
NASA Technical Reports Server (NTRS)
Teuber, D. L.; Reichmann, E. J.; Wilson, R. M.
1984-01-01
Based on the principal component analysis technique and evidence for a 22-yr double-sunspot cycle periodicity. The time series of sunspot numbers is represented as a sum of mutually orthogonal eigenvectors in the time domain. It is shown that the first two eigenvectors account for about 90 percent of the cumulative 'signal power,' and that this is sufficient for reconstruction of the raw data curve. It is also noted that the second eigenvector behaves as the time derivative of the first, and that a phase-plane plot of these eigenvectors (i.e. a plot of a variable vs. its rate of change) suggests that the sun's sunspot cycle is driven by an oscillator; the implication is that, embedded within the sun, a chronometer is at work (e.g. Dicke, 1979).
Orthogonal polynomials for refinable linear functionals
NASA Astrophysics Data System (ADS)
Laurie, Dirk; de Villiers, Johan
2006-12-01
A refinable linear functional is one that can be expressed as a convex combination and defined by a finite number of mask coefficients of certain stretched and shifted replicas of itself. The notion generalizes an integral weighted by a refinable function. The key to calculating a Gaussian quadrature formula for such a functional is to find the three-term recursion coefficients for the polynomials orthogonal with respect to that functional. We show how to obtain the recursion coefficients by using only the mask coefficients, and without the aid of modified moments. Our result implies the existence of the corresponding refinable functional whenever the mask coefficients are nonnegative, even when the same mask does not define a refinable function. The algorithm requires O(n^2) rational operations and, thus, can in principle deliver exact results. Numerical evidence suggests that it is also effective in floating-point arithmetic.
Inverse solutions for tilting orthogonal double prisms.
Li, Anhu; Ding, Ye; Bian, Yongming; Liu, Liren
2014-06-10
An analytical reverse solution and actual examples are given to show how to direct a laser beam from a pair of orthogonal prisms to given targets in free space. Considering the influences of double-prism structural parameters, a lookup table method to seek the numerical reverse solution of each prism's tilting angle is also proposed for steering the double-prism orientation to track a target position located in the near field. Some case studies, as well as a specified elliptical target trajectory scanned by the cam-based driving double prisms, exhibit the significant application values of the theoretical derivation. The analytic reverse and numerical solutions can be generalized to investigate the synthesis of scanning patterns and the controlling strategy of double-prism tilting motion, the potentials of which can be explored to perform the orientation and position tracking functions in applications of precision engineering fields. PMID:24921137
Orthogonal patterns in binary neural networks
NASA Technical Reports Server (NTRS)
Baram, Yoram
1988-01-01
A binary neural network that stores only mutually orthogonal patterns is shown to converge, when probed by any pattern, to a pattern in the memory space, i.e., the space spanned by the stored patterns. The latter are shown to be the only members of the memory space under a certain coding condition, which allows maximum storage of M=(2N) sup 0.5 patterns, where N is the number of neurons. The stored patterns are shown to have basins of attraction of radius N/(2M), within which errors are corrected with probability 1 in a single update cycle. When the probe falls outside these regions, the error correction capability can still be increased to 1 by repeatedly running the network with the same probe.
Measuring the orthogonality error of coil systems
Heilig, B.; Csontos, A.; Pajunpää, K.; White, Tim; St. Louis, B.; Calp, D.
2012-01-01
Recently, a simple method was proposed for the determination of pitch angle between two coil axes by means of a total field magnetometer. The method is applicable when the homogeneous volume in the centre of the coil system is large enough to accommodate the total field sensor. Orthogonality of calibration coil systems used for calibrating vector magnetometers can be attained by this procedure. In addition, the method can be easily automated and applied to the calibration of delta inclination–delta declination (dIdD) magnetometers. The method was tested by several independent research groups, having a variety of test equipment, and located at differing geomagnetic observatories, including: Nurmijärvi, Finland; Hermanus, South Africa; Ottawa, Canada; Tihany, Hungary. This paper summarizes the test results, and discusses the advantages and limitations of the method.
LOCC indistinguishable orthogonal product quantum states
NASA Astrophysics Data System (ADS)
Zhang, Xiaoqian; Tan, Xiaoqing; Weng, Jian; Li, Yongjun
2016-07-01
We construct two families of orthogonal product quantum states that cannot be exactly distinguished by local operation and classical communication (LOCC) in the quantum system of 2k+i ⊗ 2l+j (i, j ∈ {0, 1} and i ≥ j ) and 3k+i ⊗ 3l+j (i, j ∈ {0, 1, 2}). And we also give the tiling structure of these two families of quantum product states where the quantum states are unextendible in the first family but are extendible in the second family. Our construction in the quantum system of 3k+i ⊗ 3l+j is more generalized than the other construction such as Wang et al.’s construction and Zhang et al.’s construction, because it contains the quantum system of not only 2k ⊗ 2l and 2k+1 ⊗ 2l but also 2k ⊗ 2l+1 and 2k+1 ⊗ 2l+1. We calculate the non-commutativity to quantify the quantumness of a quantum ensemble for judging the local indistinguishability. We give a general method to judge the indistinguishability of orthogonal product states for our two constructions in this paper. We also extend the dimension of the quantum system of 2k ⊗ 2l in Wang et al.’s paper. Our work is a necessary complement to understand the phenomenon of quantum nonlocality without entanglement.
LOCC indistinguishable orthogonal product quantum states
Zhang, Xiaoqian; Tan, Xiaoqing; Weng, Jian; Li, Yongjun
2016-01-01
We construct two families of orthogonal product quantum states that cannot be exactly distinguished by local operation and classical communication (LOCC) in the quantum system of 2k+i ⊗ 2l+j (i, j ∈ {0, 1} and i ≥ j ) and 3k+i ⊗ 3l+j (i, j ∈ {0, 1, 2}). And we also give the tiling structure of these two families of quantum product states where the quantum states are unextendible in the first family but are extendible in the second family. Our construction in the quantum system of 3k+i ⊗ 3l+j is more generalized than the other construction such as Wang et al.’s construction and Zhang et al.’s construction, because it contains the quantum system of not only 2k ⊗ 2l and 2k+1 ⊗ 2l but also 2k ⊗ 2l+1 and 2k+1 ⊗ 2l+1. We calculate the non-commutativity to quantify the quantumness of a quantum ensemble for judging the local indistinguishability. We give a general method to judge the indistinguishability of orthogonal product states for our two constructions in this paper. We also extend the dimension of the quantum system of 2k ⊗ 2l in Wang et al.’s paper. Our work is a necessary complement to understand the phenomenon of quantum nonlocality without entanglement. PMID:27377310
LOCC indistinguishable orthogonal product quantum states.
Zhang, Xiaoqian; Tan, Xiaoqing; Weng, Jian; Li, Yongjun
2016-01-01
We construct two families of orthogonal product quantum states that cannot be exactly distinguished by local operation and classical communication (LOCC) in the quantum system of (2k+i) ⊗ (2l+j) (i, j ∈ {0, 1} and i ≥ j ) and (3k+i) ⊗ (3l+j) (i, j ∈ {0, 1, 2}). And we also give the tiling structure of these two families of quantum product states where the quantum states are unextendible in the first family but are extendible in the second family. Our construction in the quantum system of (3k+i) ⊗ (3l+j) is more generalized than the other construction such as Wang et al.'s construction and Zhang et al.'s construction, because it contains the quantum system of not only (2k) ⊗ (2l) and (2k+1) ⊗ (2l) but also (2k) ⊗ (2l+1) and (2k+1) ⊗ (2l+1). We calculate the non-commutativity to quantify the quantumness of a quantum ensemble for judging the local indistinguishability. We give a general method to judge the indistinguishability of orthogonal product states for our two constructions in this paper. We also extend the dimension of the quantum system of (2k) ⊗ (2l) in Wang et al.'s paper. Our work is a necessary complement to understand the phenomenon of quantum nonlocality without entanglement. PMID:27377310
The second level TOF trigger for the Obelix experiment
Lai, A.; Musa, L.; Serci, S. )
1994-08-01
The authors describe a CAMAC module, named TRIG-GERK, performing the function of second level trigger in the TOF apparatus of the PS201 experiment at CERN. The module is realized on a single board and is implemented by using three XILINX PGAs. The internal architecture of the module as well as its programming capabilities and mode of usage is described.
... may be prescribed for other uses; ask your doctor or pharmacist for more information. ... Before using lacosamide injection,tell your doctor and pharmacist if you are allergic to lacosamide, any other medications, or any of the ingredients in lacosamide injection. Ask your pharmacist for a ...
... lines under the skin skin depressions at the injection site increased body fat or movement to different areas of your body inappropriate happiness difficulty falling asleep or staying asleep extreme ... increased appetite injection site pain or redness Some side effects can ...
... you that you will need to receive a vitamin B12 injection no more than 10 weeks before your first ... tests to check your body's response to pralatrexate injection.Ask your ... such as vitamins, minerals, or other dietary supplements. You should bring ...
... lack of vitamin B12 or inability to absorb vitamin B12. Your doctor will not prescribe leucovorin injection to treat this type of anemia.tell your ... tests to check your body's response to leucovorin injection.It is ... such as vitamins, minerals, or other dietary supplements. You should bring ...
... and colorless. The liquid may contain small white particles, but should not contain large or colored particles. Do not use a syringe or dosing pen ... liquid is cloudy or contains large or colored particles.The best place to inject etanercept injection is ...
Wittich, Christopher M.; Ficalora, Robert D.; Mason, Thomas G.; Beckman, Thomas J.
2009-01-01
Patients commonly present to primary care physicians with musculoskeletal symptoms. Clinicians certified in internal medicine must be knowledgeable about the diagnosis and management of musculoskeletal diseases, yet they often receive inadequate postgraduate training on this topic. The musculoskeletal problems most frequently encountered in our busy injection practice involve, in decreasing order, the knees, trochanteric bursae, and glenohumeral joints. This article reviews the clinical presentations of these problems. It also discusses musculoskeletal injections for these problems in terms of medications, indications, injection technique, and supporting evidence from the literature. Experience with joint injection and the pharmacological principles described in this article should allow primary care physicians to become comfortable and proficient with musculoskeletal injections. PMID:19720781
Sabo, Martin; Matejčík, Štefan
2012-06-19
We demonstrate the application of corona discharge ion mobility spectrometry with orthogonal acceleration time of flight mass spectrometry (CD IMS-oaTOF) for volatile organic compounds (VOCs) monitoring. Two-dimensional (2D) IMS-oaTOF spectra of VOCs were recorded in nearly real time. The corona discharge atmospheric pressure chemical ionization (APCI) source was operated in positive mode in nitrogen and air. The CD ion source generates in air H(3)O(+)(H(2)O)(n) and NO(+). The NO(+) offers additional possibility for selective ionization and for an increase of the sensitivity of monoaromatic compounds. In addition to H(3)O(+)(H(2)O)(n) and NO(+), we have carried out ionization of VOCs using acetone as dopant gas ((CH(3))(2)COH(+)). Sixteen model VOCs (tetrahydrofuran, butanol, n-propanol, iso-propano, acetone, methanol, ethanol, toluene, benzene, amomnia, dioxan, triethylamine, acetonitrile, formaldehyde, m-xylene, 2,2,2-trifluoroethylamine) were tested using these ionization techniques. PMID:22594852
The Gibbs Phenomenon for Series of Orthogonal Polynomials
ERIC Educational Resources Information Center
Fay, T. H.; Kloppers, P. Hendrik
2006-01-01
This note considers the four classes of orthogonal polynomials--Chebyshev, Hermite, Laguerre, Legendre--and investigates the Gibbs phenomenon at a jump discontinuity for the corresponding orthogonal polynomial series expansions. The perhaps unexpected thing is that the Gibbs constant that arises for each class of polynomials appears to be the same…
A new method testing the orthogonality of different protecting groups.
Ágoston, Károly; Ágoston, Ágnes; Dorgan, Colin R; Fügedi, Péter
2015-12-11
A new test was elaborated to identify a new set of orthogonal protecting groups. With the developed method eight different protecting groups were tested under various deprotection conditions and the complex reaction mixtures were analysed by HPLC. The developed method allows for quick identification of orthogonality using simple model structures. PMID:26580711
Least-Squares Adaptive Control Using Chebyshev Orthogonal Polynomials
NASA Technical Reports Server (NTRS)
Nguyen, Nhan T.; Burken, John; Ishihara, Abraham
2011-01-01
This paper presents a new adaptive control approach using Chebyshev orthogonal polynomials as basis functions in a least-squares functional approximation. The use of orthogonal basis functions improves the function approximation significantly and enables better convergence of parameter estimates. Flight control simulations demonstrate the effectiveness of the proposed adaptive control approach.
Experimental quantum cryptography scheme based on orthogonal states: preliminary results
NASA Astrophysics Data System (ADS)
Avella, Alessio; Brida, Giorgio; Degiovanni, Ivo P.; Genovese, Marco; Gramegna, Marco; Traina, Paolo
2010-04-01
Since, in general, non-orthogonal states cannot be cloned, any eavesdropping attempt in a Quantum Communication scheme using non-orthogonal states as carriers of information introduces some errors in the transmission, leading to the possibility of detecting the spy. Usually, orthogonal states are not used in Quantum Cryptography schemes since they can be faithfully cloned without altering the transmitted data. Nevertheless, L. Goldberg and L. Vaidman [Phys. Rev. Lett. 75 (7), pp. 12391243, 1995] proposed a protocol in which, even if the data exchange is realized using two orthogonal states, any attempt to eavesdrop is detectable by the legal users. In this scheme the orthogonal states are superpositions of two localized wave packets which travel along separate channels, i.e. two different paths inside a balanced Mach-Zehnder interferometer. Here we present an experiment realizing this scheme.
Iiyoshi, A.; Vogoshi, S.
1983-12-01
The Plasma Physics Laboratory and the Dept. of Electrical Engineering report on three types of pellet injectors which have different applications: injection of a pellet into a magnetic bottle for magnetic confinement; injection of a pellet into a vacuum chamber for an inertial confinement experiment; and injection of a pellet into a magnetic bottle where the pellet is ionized by high-power laser irradiation for target plasma production. The requirements of pellet injectors are summarized in a table. Theoretical studies on pellet ablation in hot plasma and ablated particle diffusion are underway.
Approximate Orthogonal Sparse Embedding for Dimensionality Reduction.
Lai, Zhihui; Wong, Wai Keung; Xu, Yong; Yang, Jian; Zhang, David
2016-04-01
Locally linear embedding (LLE) is one of the most well-known manifold learning methods. As the representative linear extension of LLE, orthogonal neighborhood preserving projection (ONPP) has attracted widespread attention in the field of dimensionality reduction. In this paper, a unified sparse learning framework is proposed by introducing the sparsity or L1-norm learning, which further extends the LLE-based methods to sparse cases. Theoretical connections between the ONPP and the proposed sparse linear embedding are discovered. The optimal sparse embeddings derived from the proposed framework can be computed by iterating the modified elastic net and singular value decomposition. We also show that the proposed model can be viewed as a general model for sparse linear and nonlinear (kernel) subspace learning. Based on this general model, sparse kernel embedding is also proposed for nonlinear sparse feature extraction. Extensive experiments on five databases demonstrate that the proposed sparse learning framework performs better than the existing subspace learning algorithm, particularly in the cases of small sample sizes. PMID:25955995
Directional lapped orthogonal transform: theory and design.
Muramatsu, Shogo; Han, Dandan; Kobayashi, Tomoya; Kikuchi, Hisakazu
2012-05-01
This paper proposes a directional design method of 2-D nonseparable linear-phase paraunitary filter banks. The proposed method is based on a lattice structure consisting of the 2-D separable DCT block and nonseparable support extension processes. Because of the nonseparability, the bases are allowed to be directional with the critically fixed subsampling, overlapping, orthogonal, symmetric, real-valued, and compact support properties. First, a novel vanishing moment (VM) condition is introduced as a suitable directional constraint, where the moment is referred to as the trend VM. The condition forces wavelet filters, i.e., high-pass and bandpass filters, to annihilate trend-surface components. Second, some theoretical properties of TVMs are discussed for general 2-D paraunitary systems, and then, the properties are applied to the lattice parameters. In order to verify the significance, several design examples are shown, the trend-surface annihilation properties are numerically confirmed, and the denoising capability is evaluated for images through shrinkage. It is shown that our proposed transforms yield perceptually preferable results. PMID:22231175
Anatomy of lithosphere necking during orthogonal rifting
NASA Astrophysics Data System (ADS)
Nestola, Yago; Cavozzi, Cristian; Storti, Fabrizio
2013-04-01
The evolution of lithosphere necking is a fundamental parameter controlling the structural architecture and thermal-state of rifted margin. The necking shape depends on several parameters, including the extensional strain-rate and thermal layering of the lithosphere. Despite a large number of analogue and numerical modelling studies on lithosphere extension, a quantitative description of the evolution of necking through time is still lacking. We used analogue modelling to simulate in three-dimension the progression of lithosphere thinning and necking during orthogonal rifting. In our models we simulated a typical "cold and young" 4-layer lithosphere stratigraphy: brittle upper crust (loose quartz sand), ductile lower crust (silicon-barite mixture), brittle upper mantle (loose quartz sand), and ductile lower mantle (silicon-barite mixture). The experimental lithosphere rested on a glucose syrup asthenosphere. We monitored model evolution by periodic and coeval laser scanning of both the surface topography and the lithosphere base. After model completion, each of the four layers was removed and the top of the underlying layer was scanned. This technical approach allowed us to quantify the evolution in space and time of the thinning factors for both the whole lithosphere (βz) and the crust (γ). The area of incremental effective stretching (βy) parallel to the extensional direction was obtained from the βz maps.
The Representation and Parametrization of Orthogonal Matrices.
Shepard, Ron; Brozell, Scott R; Gidofalvi, Gergely
2015-07-16
Four representations and parametrizations of orthogonal matrices Q ∈ R(m×n) in terms of the minimal number of essential parameters {φ} are discussed: the exponential representation, the Householder reflector representation, the Givens rotation representation, and the rational Cayley transform representation. Both square n = m and rectangular n < m situations are considered. Two separate kinds of parametrizations are considered: one in which the individual columns of Q are distinct, the Stiefel manifold, and the other in which only span(Q) is significant, the Grassmann manifold. The practical issues of numerical stability, continuity, and uniqueness are discussed. The computation of Q in terms of the essential parameters {φ}, and also the extraction of {φ} for a given Q are considered for all of the parametrizations. The transformation of gradient arrays between the Q and {φ} variables is discussed for all representations. It is our hope that developers of new methods will benefit from this comparative presentation of an important but rarely analyzed subject. PMID:25946418
Genuinely multipartite entangled states and orthogonal arrays
NASA Astrophysics Data System (ADS)
Goyeneche, Dardo; Życzkowski, Karol
2014-08-01
A pure quantum state of N subsystems with d levels each is called k-multipartite maximally entangled state, which we call a k-uniform state, if all its reductions to k qudits are maximally mixed. These states form a natural generalization of N-qudit Greenberger-Horne-Zeilinger states which belong to the class 1-uniform states. We establish a link between the combinatorial notion of orthogonal arrays and k-uniform states and prove the existence of several classes of such states for N-qudit systems. In particular, known Hadamard matrices allow us to explicitly construct 2-uniform states for an arbitrary number of N >5 qubits. We show that finding a different class of 2-uniform states would imply the Hadamard conjecture, so the full classification of 2-uniform states seems to be currently out of reach. Furthermore, we establish links between the existence of k-uniform states and classical and quantum error correction codes and provide a graph representation for such states.
A reduced basis localized orthogonal decomposition
NASA Astrophysics Data System (ADS)
Abdulle, Assyr; Henning, Patrick
2015-08-01
In this work we combine the framework of the Reduced Basis method (RB) with the framework of the Localized Orthogonal Decomposition (LOD) in order to solve parametrized elliptic multiscale problems. The idea of the LOD is to split a high dimensional Finite Element space into a low dimensional space with comparably good approximation properties and a remainder space with negligible information. The low dimensional space is spanned by locally supported basis functions associated with the node of a coarse mesh obtained by solving decoupled local problems. However, for parameter dependent multiscale problems, the local basis has to be computed repeatedly for each choice of the parameter. To overcome this issue, we propose an RB approach to compute in an "offline" stage LOD for suitable representative parameters. The online solution of the multiscale problems can then be obtained in a coarse space (thanks to the LOD decomposition) and for an arbitrary value of the parameters (thanks to a suitable "interpolation" of the selected RB). The online RB-LOD has a basis with local support and leads to sparse systems. Applications of the strategy to both linear and nonlinear problems are given.
Quantum nonlocality of multipartite orthogonal product states
NASA Astrophysics Data System (ADS)
Xu, Guang-Bao; Wen, Qiao-Yan; Qin, Su-Juan; Yang, Ying-Hui; Gao, Fei
2016-03-01
Local distinguishability of orthogonal quantum states is an area of active research in quantum information theory. However, most of the relevant results are about local distinguishability in bipartite Hilbert space and very little is known about the multipartite case. In this paper we present a generic method to construct a completable n -partite (n ≥3 ) product basis with only 2 n members, which exhibits nonlocality without entanglement with n parties, each holding a system of any finite dimension. We give an effective proof of the nonlocality of the completable multipartite product basis. In addition, we construct another incomplete multipartite product basis with a smaller number of members that cannot be distinguished by local operations and classical communication in a d1⊗d2⊗⋯⊗dn quantum system, where n ≥3 and di≥2 for i =1 ,2 ,...,n . The results can lead to a better understanding of the phenomenon of nonlocality without entanglement in any multipartite quantum system.
... has not improved when treated with other medications, rheumatoid arthritis (a condition in which the body attacks its ... continues. When certolizumab injection is used to treat rheumatoid arthritis, it is usually given every other week and ...
... dose of ramucirumab injection. Tell your doctor or nurse if you experience any of the following while you receive ramucirumab: uncontrollable shaking of a part of the body; back pain or spasms; chest pain and tightness; chills; flushing; ...
... organs where eggs are formed) and small cell lung cancer (a type of cancer that begins in the ... topotecan injection is used to treat ovarian or lung cancer, it is usually given once a day for ...
... antibiotic, to help treat your infection. The drug will be either injected directly into a vein through ... catheter or added to an intravenous fluid that will drip through a needle or catheter into a ...
... medications to relieve pain in people with advanced prostate cancer who did not respond to other medications. Mitoxantrone ... doses). When mitoxantrone injection is used to treat prostate cancer, it is usually given once every 21 days. ...
... this medicine each month during RSV season. Your health care provider will let you know when the monthly injections are no longer needed.Your child's health care provider (doctor, nurse, or pharmacist) may measure ...
Terbutaline injection is used to treat wheezing, shortness of breath, coughing, and chest tightness caused by asthma, chronic bronchitis, and emphysema. Terbutaline is in a class of medications called beta ...
... of the body and causes pain, heavy or irregular menstruation [periods], and other symptoms). Leuprolide injection (Lupron ... mention any of the following: certain medications for irregular heartbeat such as amiodarone (Cordarone), disopyramide (Norpace), procainamide ( ...
... accompanied by nausea and sensitivity to sound and light). Sumatriptan injection is also used to treat the ... children. Store it at room temperature, away from light, excess heat, and moisture (not in the bathroom). ...
... contraceptives (birth control pills, patches, rings, injections, or implants); niacin (Niacor, Niaspan, Slo-Niacin); octreotide (Sandostatin);oral ... cramps abnormal heartbeat large weight gain in a short period of time swelling of the arms, hands, ...
... had a serious allergic reaction (difficulty breathing or swallowing or swelling of the face, throat, tongue, lips, ... the face, throat, tongue, lips, or eyes difficulty swallowing or breathing Fondaparinux injection may cause other side ...
... course of disease where symptoms flare up from time to time) of multiple sclerosis (MS; a disease in which ... injections. Before you use daclizumab yourself the first time, read the written instructions that come with it. ...
... emotions). Haloperidol injection is also used to control motor tics (uncontrollable need to repeat certain body movements) ... people who have Tourette's disorder (condition characterized by motor or verbal tics). Haloperidol is in a class ...
... causes pain, swelling, and damage) including the following: Crohn's disease (a condition in which the body attacks the ... home. When certolizumab injection is used to treat Crohn's disease, it is usually given every two weeks for ...
... prevent episodes of symptoms in people who have Crohn's disease (a condition in which the body attacks the ... If you are receiving natalizumab injection to treat Crohn's disease, your symptoms should improve during the first few ...
... blood infections or serious skin infections caused by bacteria. Daptomycin injection is in a class of medications called cyclic lipopeptide antibiotics. It works by killing bacteria. Antibiotics will not work for treating colds, flu, ...
... is used to treat certain infections caused by bacteria. Ciprofloxacin injection is also used to prevent or ... of antibiotics called fluoroquinolones. It works by killing bacteria that cause infections. Antibiotics will not work for ...
... treat certain serious infections that are caused by bacteria such as meningitis (infection of the membranes that ... medications called aminoglycoside antibiotics. It works by killing bacteria.Antibiotics such as gentamicin injection will not work ...
... abdominal (stomach area) infections, that are caused by bacteria. It is also used for the prevention of ... medications called carbapenem antibiotics. It works by killing bacteria.Antibiotics such as ertapenem injection will not work ...
... is used to treat certain infections caused by bacteria including pneumonia, and skin, urinary tract, and kidney ... medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as cefepime injection will not work ...
... is used to treat certain infections caused by bacteria such as gonorrhea (a sexually transmitted disease), pelvic ... medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as ceftriaxone injection will not work ...
... skin, and abdominal (stomach area) infections caused by bacteria. Moxifloxacin injection is in a class of antibiotics called fluoroquinolones. It works by killing the bacteria that cause infections. Antibiotics will not work against ...
... infections and pneumonia (lung infection) caused by certain bacteria. Ceftaroline is in a class of medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as ceftaroline injection will not work ...
... treat certain serious infections that are caused by bacteria such as meningitis (infection of the membranes that ... medications called aminoglycoside antibiotics. It works by killing bacteria.Antibiotics such as tobramycin injection will not work ...
... is used to treat certain infections caused by bacteria including skin, bone, joint, genital, blood, heart valve, ... medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as cefazolin injection will not work ...
... is used to treat certain infections caused by bacteria including pneumonia and other lower respiratory tract (lung) ... medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as cefotaxime injection will not work ...
... treat certain serious infections that are caused by bacteria such as meningitis (infection of the membranes that ... medications called aminoglycoside antibiotics. It works by killing bacteria.Antibiotics such as amikacin injection will not work ...
... to treat certain infections that are caused by bacteria such as meningitis (infection of the membranes that ... of medications called penicillins. It works by killing bacteria.Antibiotics such as ampicillin injection will not work ...
... is used to treat certain infections caused by bacteria including pneumonia and other lower respiratory tract (lung) ... medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as cefuroxime injection will not work ...
... medications called glycopeptide antibiotics. It works by killing bacteria that cause infections.Antibiotics such as vancomycin injection ... infection may not be completely treated and the bacteria may become resistant to antibiotics.
... is used to treat certain infections caused by bacteria including pneumonia and other lower respiratory tract (lung) ... medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as ceftazidime injection will not work ...
... serious skin infections caused by certain types of bacteria. Telavancin injection is in a class of medications ... antibiotics. It works by stopping the growth of bacteria. Antibiotics will not work for colds, flu, or ...
... injection, prefilled syringes containing diluent (liquid to be mixed with teduglutide powder), needles to attach to the diluent syringe, dosing syringes with needles attached, and alcohol swab pads. Throw away needles, syringes, and vials ...
... is in a class of medications called cephamycin antibiotics. It works by killing bacteria.Antibiotics such as cefoxitin injection will not work for colds, flu, or other viral infections. Taking antibiotics when they are not needed increases your risk ...
... medications called penicillins. It works by killing bacteria.Antibiotics such as nafcillin injection will not work for colds, flu, or other viral infections. Taking antibiotics when they are not needed increases your risk ...
... is in a class of medications called cephalosporin antibiotics. It works by killing bacteria.Antibiotics such as cefepime injection will not work for colds, flu, or other viral infections. Using antibiotics when they are not needed increases your risk ...
... medications called penicillins. It works by killing bacteria.Antibiotics such as oxacillin injection will not work for colds, flu, or other viral infections. Taking antibiotics when they are not needed increases your risk ...
... severe allergic reactions. It is used in the management of certain types of edema (fluid retention and ... needed for normal body functioning) and in the management of certain types of shock. Dexamethasone injection is ...
... treat a certain type of non-small-cell lung cancer that has spread to nearby tissues or to ... successfully with other medications for non-small-cell lung cancer. Pembrolizumab injection is in a class of medications ...
... Ibandronate is in a class of medications called bisphosphonates. It works by preventing bone breakdown and increasing ... while receiving this medication.Being treated with a bisphosphonate medication such as ibandronate injection for osteoporosis may ...
... asthma attacks (sudden episodes of wheezing, shortness of breath, and trouble breathing) in people with allergic asthma ( ... receiving a dose of omalizumab injection shortness of breath coughing up blood skin sores severe pain, numbness ...
... chest pain; shortness of breath; dizziness; loss of consciousness; or fast, irregular, or pounding heartbeat.Keep all appointments with your doctor and the laboratory.Talk to your doctor about the risks of receiving necitumumab injection.
... receiving cancer chemotherapy medications. Dolasetron is in a class of medications called serotonin 5-HT3 receptor antagonists. ... stiff or twitching muscles seizures coma (loss of consciousness) Dolasetron injection may cause other side effects. Call ...
... also used together with other medications to treat cervical cancer (cancer that begins in the opening of the ... days. When topotecan injection is used to treat cervical cancer, it is usually given once a day for ...
Ertapenem injection is used to treat certain serious infections, including pneumonia and urinary tract, skin, diabetic foot, ... for the prevention of infections following colorectal surgery. Ertapenem is in a class of medications called carbapenem ...
... immediate-release injection is also used to control diarrhea and flushing caused by carcinoid tumors (slow-growing ... symptoms are severe or do not go away: diarrhea constipation pale, bulky, foul-smelling stools constantly feeling ...
... injection may cause serious allergic reactions during an infusion and for 2 hours afterward. A doctor or ... the following symptoms during or shortly after your infusion: hives; rash; itching; swelling of the face, eyes, ...
... injection may cause serious allergic reactions during an infusion and for several hours afterward. A doctor or ... of the following symptoms during or after your infusion: rash; itching; swelling of the face, eyes, mouth, ...
... as a solution (liquid) to be given by infusion (injected into a vein). It is usually given ... doctor or nurse in a doctor's office or infusion center. Panitumumab is usually given once every 2 ...
... is in a class of medications called monoclonal antibodies with radioisotopes. It works by attaching to cancer ... you receive tositumomab injection, your body may develop antibodies (substances in the blood that help the immune ...
... is in a class of medications called monoclonal antibodies with radioisotopes. It works by attaching to cancer ... you receive ibritumomab injection, your body may develop antibodies (substances in the blood that help the immune ...
Temozolomide is used to treat certain types of brain tumors. Temozolomide is in a class of medications called ... injected once a day. For some types of brain tumors, temozolomide is given daily for 42 to 49 ...
... to treat certain serious infections including community acquired pneumonia (a lung infection that developed in a person ... Tigecycline injection should not be used to treat pneumonia that developed in people who were in a ...
... injection is also used in combination with opioid (narcotic) medications to relieve moderate to severe pain. Acetaminophen is in a class of medications called analgesics (pain relievers) and antipyretics (fever reducers). It works by changing ...
... certain side effects that may be caused by chemotherapy medications. Dexrazoxane injection (Zinecard) is used to prevent ... tissues that may be caused when an anthracycline chemotherapy medication such as daunorubicin (Daunoxome, Cerubidine), doxorubicin (Doxil), ...
... menstrual periods), who have an increased risk for fractures (broken bones) or who cannot take or did ... receiving certain treatments that increase their risk for fractures. Denosumab injection (Xgeva) is used to reduce fractures ...
... of disability in patients with certain forms of multiple sclerosis (MS). Mitoxantrone injection is also used together with steroid ... a class of medications called anthracenediones. Mitoxantrone treats MS by stopping certain cells of the immune system ...
... and pharmacist if you are allergic to dexrazoxane injection or any other medications.tell your doctor and pharmacist what prescription and nonprescription medications, vitamins, nutritional supplements, and herbal products you are taking ...
... for at least 5 days after receiving oritavancin injection.tell your doctor and pharmacist what other prescription and nonprescription medications, vitamins, nutritional supplements, and herbal products you are taking ...
Ferumoxytol injection is used to treat iron-deficiency anemia (a lower than normal number of red blood cells due to too little iron) in adults with chronic kidney disease (damage to the kidneys which may worsen over ...
... month. Exenatide extended-release solution is injected once weekly at any time of day without regard to ... you remember it and then continue your regular weekly schedule. However, if there are less than 3 ...
... injection is used to treat fungal infections, including yeast infections of the mouth, throat, esophagus (tube leading from ... by fungus. Fluconazole is also used to prevent yeast infections in patients who are likely to become infected ...
Mipomersen injection is used to decrease levels of cholesterol and other fatty substances in the blood in people who have homozygous familial hypercholesterolemia (HoFH; a rare inherited condition that ...
... pneumonia and other lower respiratory tract (lung) infections; meningitis (infection of the membranes that surround the brain ... hearing loss, if you are being treated for meningitis Cefuroxime injection may cause other side effects. Call ...
... cancer cells in preparation for a bone marrow transplant. Busulfan is in a class of medications called ... a total of 16 doses) before bone marrow transplant.Busulfan injection may cause seizures during therapy with ...
... injection is used to treat constipation caused by opioid (narcotic) pain medications in patients with advanced illnesses ... a class of medications called peripherally acting mu-opioid receptor antagonists. It works by protecting the bowel ...
... treatment.You may receive methylprednisolone injection in a hospital or medical facility, or you may be given ... doctor or if you are admitted to a hospital. It is also important information to carry with ...
... have.You may receive ampicillin injection in a hospital or you may administer the medication at home. ... doctor or if you are admitted to a hospital. It is also important information to carry with ...
... bleeding fever, cough, flu-like symptoms, muscle aches, burning on urination, worsening skin problems, and other signs of infection rash blistering or peeling skin Romidepsin injection may cause other side effects. Call your doctor if you have any unusual ...
... the pancreas and small intestine that caused increased production of stomach acid). Ranitidine injection is in a ... your doctor and the laboratory. Your doctor may order certain lab tests to check your body's response ...
... injection is for intravenous (into a vein) use only. Giving ganciclovir through intramuscular (into a muscle) or ... the storage of ganciclovir solution. Store your medication only as directed. Make sure you understand what you ...
... syndrome in people who need additional nutrition or fluids from intravenous (IV) therapy. Teduglutide injection is in ... analogs. It works by improving the absorption of fluids and nutrients in the intestines.
Olanzapine extended-release injection is used to treat schizophrenia (a mental illness that causes disturbed or unusual ... treat episodes of agitation in people who have schizophrenia or in people who have bipolar I disorder ( ...
... release (long-acting) injection is used to treat schizophrenia (a mental illness that causes disturbed or unusual ... do not already have diabetes. If you have schizophrenia, you are more likely to develop diabetes than ...
... injections (Abilify Maintena, Aristada) are used to treat schizophrenia (a mental illness that causes disturbed or unusual ... treat episodes of agitation in people who have schizophrenia or in people who have bipolar I disorder ( ...
... to see if you need to receive any vaccinations. It is important to have all vaccines appropriate ... treatment with secukinumab injection. Do not have any vaccinations during your treatment without talking to your doctor. ...
... fat in the stomach area in adults with human immunodeficiency virus (HIV) who have lipodystrophy (increased body ... injection is in a class of medications called human growth hormone-releasing factor (GRF) analogs. It works ...
... emergency medical treatment to reverse the life-threatening effects of a known or suspected opiate (narcotic) overdose. ... is also used after surgery to reverse the effects of opiates given during surgery. Naloxone injection is ...
... Methotrexate injection is also used to treat severe psoriasis (a skin disease in which red, scaly patches ... slowing the growth of cancer cells. Methotrexate treats psoriasis by slowing the growth of skin cells to ...
... sometimes are accompanied by nausea and sensitivity to sound and light). Sumatriptan injection is also used to ... stomach pain sudden weight loss paleness or blue color of the fingers and toes shortness of breath ...
... injection is in a class of medications called RANK ligand inhibitors. It works by decreasing bone breakdown ... medicines you are taking, as well as any products such as vitamins, minerals, or other dietary supplements. ...
... for CML and can no longer benefit from these medications or cannot take these medications due to side effects. Omacetaxine injection is ... side effects. Tell your doctor if any of these symptoms are severe or do not go away: ...
... is used with other medications to prevent immediate transplant rejection (attack of the transplanted organ by the immune system of the person receiving the organ) in people who are receiving kidney transplants. Basiliximab injection is in a class of medications ...
... is used to relieve symptoms caused by slow stomach emptying in people who have diabetes. These symptoms include ... When metoclopramide injection is used to treat slowed stomach emptying due to diabetes, it may be given up ...
Manna, Sugata; Bhandoola, Avinash
2016-01-01
Intrathymic injection is used in several T cell-associated immunological studies to deliver cells or other substances directly into the thymus. Here, we describe the intrathymic injection procedure involving surgical incision of the mouse with or without a thoracotomy. Though this procedure can result in poor recovery, postsurgical complications, and distress to the animal, it is actually a simple procedure that can be carried out relatively easily and quickly with experience. PMID:26294410
Bayesian Peptide Peak Detection for High Resolution TOF Mass Spectrometry
Zhang, Jianqiu; Zhou, Xiaobo; Wang, Honghui; Suffredini, Anthony; Zhang, Lin; Huang, Yufei; Wong, Stephen
2011-01-01
In this paper, we address the issue of peptide ion peak detection for high resolution time-of-flight (TOF) mass spectrometry (MS) data. A novel Bayesian peptide ion peak detection method is proposed for TOF data with resolution of 10 000–15 000 full width at half-maximum (FWHW). MS spectra exhibit distinct characteristics at this resolution, which are captured in a novel parametric model. Based on the proposed parametric model, a Bayesian peak detection algorithm based on Markov chain Monte Carlo (MCMC) sampling is developed. The proposed algorithm is tested on both simulated and real datasets. The results show a significant improvement in detection performance over a commonly employed method. The results also agree with expert’s visual inspection. Moreover, better detection consistency is achieved across MS datasets from patients with identical pathological condition. PMID:21544266
ASTROPHYSICS AT n{sub T}OF FACILITY
Tagliente, G.; Colonna, N.; Marrone, S.; Tagliente, G.; Terlizzi, R.; Abbondanno, U.; Belloni, F.; Fujii, K.; Milazzo, P. M.; Aerts, G.; Andriamonje, S.; Berthoumieux, E.; Dridi, W.; Ferrant, L.; Gunsing, F.; Pancin, J.; Perrot, L.; Plukis, A.; Stephan, C.; Tassan-Got, L.
2010-08-04
Heavy elements with Z{>=}30 are made by neutron capture reactions during stellar He burning and presumably in supernovae. This contribution deals mainly with the slow neutron capture (s) process which is responsible for about one half of the abundances in the mass region between Fe and Bi. The slow time scale implies that the reaction path of this process involves mostly stable isotopes which can be studied in detail in laboratory experiments. The neutron time of flight (n{sub T}OF) facility at CERN is a neutron spallation source, its white neutron energy spectrum ranges from thermal to several MeV, covering the full energy range of interest for nuclear astrophysics, in particular for measurements of the neutron capture cross section required in s-process nucleosynthesis. This contribution gives an overview on the astrophysical program made at n{sub T}OF facility, the results and the implications will be considered.
Automatic control system for the COSY-TOF vacuum system
NASA Astrophysics Data System (ADS)
Abdel-Bary, M.; Abdel-Samad, S.; Kilian, K.
2005-02-01
An automatic control system (ACS) for the vacuum installations of the Time-Of-Flight (TOF) spectrometer at the COoler SYnchrotron (COSY) was developed. SIMATIC S7 software and hardware was used to build the control program and Windows Control Center (WinCC) software was taken to make the human-machine interface. The ACS can work automatically or manually. In both cases there are safety interlocks in order to prevent damage of the accelerator or detector components. The testing was firstly done by using external signals and a simulator in WinCC, then secondly we connected it to a real, small vacuum system. The communication between the SIMATIC S7 hardware and the WinCC, which run in a PC computer, is done by Multi Point Interface (MPI). The ACS is now installed in the COSY-TOF experiment and runs routinely.
Wang, Huai-Yung; Chi, Yu-Chieh; Lin, Gong-Ru
2016-08-01
A novel millimeter-wave radio over fiber (MMW-RoF) link at carrier frequency of 35-GHz is proposed with the use of remotely beating MMW generation from reference master and injected slave colorless laser diode (LD) carriers at orthogonally polarized dual-wavelength injection-locking. The slave colorless LD supports lasing one of the dual-wavelength master modes with orthogonal polarizations, which facilitates the single-mode direct modulation of the quadrature amplitude modulation (QAM) orthogonal frequency division multiplexing (OFDM) data. Such an injected single-carrier encoding and coupled dual-carrier transmission with orthogonal polarization effectively suppresses the cross-heterodyne mode-beating intensity noise, the nonlinear modulation (NLM) and four-wave mixing (FWM) sidemodes during injection locking and fiber transmission. In 25-km single-mode fiber (SMF) based wireline system, the dual-carrier under single-mode encoding provides baseband 24-Gbit/s 64-QAM OFDM transmission with an error vector magnitude (EVM) of 8.8%, a bit error rate (BER) of 3.7 × 10^{-3}, a power penalty of <1.5 dB. After remotely self-beating for wireless transmission, the beat MMW carrier at 35 GHz can deliver the passband 16-QAM OFDM at 4 Gbit/s to show corresponding EVM and BER of 15.5% and 1.4 × 10^{-3}, respectively, after 25-km SMF and 1.6-m free-space transmission. PMID:27505734
Characterization of Enterobacteria using MALDI-TOF mass spectrometry.
Pribil, Patrick; Fenselau, Catherine
2005-09-15
A method is proposed for the rapid classification of Gram-negative Enterobacteria using on-slide solubilization and trypsin digestion of proteins, followed by MALDI-TOF MS analysis. Peptides were identified from tryptic digests using microsequencing by tandem mass spectrometry and database searches. Proteins from the outer membrane family (OMP) were consistently identified in the Enterobacteria Escherichia coli, Enterobacter cloacae, Erwinia herbicola, and Salmonella typhimurium. Database searches indicate that these OMP peptides observed are unique to the Enterobacteria order. PMID:16159146
Polar Plate Theory for Orthogonal Anisotropy
NASA Technical Reports Server (NTRS)
Bailey, Michelle D.; Bower, Mark V.
2000-01-01
Laminated fiber-reinforced (or filamentary) composites are used today for their high strength-to-weight and stiffness-to-weight ratios. However, because of the anisotropic behavior of composites, determining the response on a macroscopic scale is challenging. This is particularly evident in the evaluation of the governing differential equations of a circular disk with the fibers of the lamina oriented with rectilinear orthogonality. This includes any situation involving a composite plate of circular geometry in which out-of-plane displacements due to load are desired, such as fastener pull through loading of a composite plate. Current analysis techniques use numerical methods with rectilinear coordinate systems to solve problems with circular geometry. These analyses over predict plate stiffness by 20% and underpredict failure by 70%. Consequently, there is a need to transform classical composite plate theory to a polar coordinate system. In order to better analyze structures with circular geometries the classical composite plate equations are transformed into the plate equations for a rectilinearly anisotropic composite in polar coordinates. A composite plate is typically a laminate of fibers in rectilinear directions. Subsequent to the lay-tip the necessary geometry is cut out of a rectangular plate. In a similar manner, the derivation of the plate equation starts with the fundamental definitions of strain, displacement and curvature and incorporates the material property angular dependence into the equilibrium equations for a differential polar element. In the transformed state, the stiffness coefficients are no longer constant, adding to the complexity of the governing differential equations. This paper discusses the new derivation and evaluation of the plate equations for a circular composite disk with orthogonal rectilinear anisotropy. The resultant new three partial differential equations, which describe the circular anisotropic plate, can be used to
Ocean Models and Proper Orthogonal Decomposition
NASA Astrophysics Data System (ADS)
Salas-de-Leon, D. A.
2007-05-01
The increasing computational developments and the better understanding of mathematical and physical systems resulted in an increasing number of ocean models. Long time ago, modelers were like a secret organization and recognize each other by using secret codes and languages that only a select group of people was able to recognize and understand. The access to computational systems was reduced, on one hand equipment and the using time of computers were expensive and restricted, and on the other hand, they required an advance computational languages that not everybody wanted to learn. Now a days most college freshman own a personal computer (PC or laptop), and/or have access to more sophisticated computational systems than those available for research in the early 80's. The resource availability resulted in a mayor access to all kind models. Today computer speed and time and the algorithms does not seem to be a problem, even though some models take days to run in small computational systems. Almost every oceanographic institution has their own model, what is more, in the same institution from one office to the next there are different models for the same phenomena, developed by different research member, the results does not differ substantially since the equations are the same, and the solving algorithms are similar. The algorithms and the grids, constructed with algorithms, can be found in text books and/or over the internet. Every year more sophisticated models are constructed. The Proper Orthogonal Decomposition is a technique that allows the reduction of the number of variables to solve keeping the model properties, for which it can be a very useful tool in diminishing the processes that have to be solved using "small" computational systems, making sophisticated models available for a greater community.
Exploratory analysis of TOF-SIMS data from biological surfaces
NASA Astrophysics Data System (ADS)
Vaidyanathan, Seetharaman; Fletcher, John S.; Henderson, Alex; Lockyer, Nicholas P.; Vickerman, John C.
2008-12-01
The application of multivariate analytical tools enables simplification of TOF-SIMS datasets so that useful information can be extracted from complex spectra and images, especially those that do not give readily interpretable results. There is however a challenge in understanding the outputs from such analyses. The problem is complicated when analysing images, given the additional dimensions in the dataset. Here we demonstrate how the application of simple pre-processing routines can enable the interpretation of TOF-SIMS spectra and images. For the spectral data, TOF-SIMS spectra used to discriminate bacterial isolates associated with urinary tract infection were studied. Using different criteria for picking peaks before carrying out PC-DFA enabled identification of the discriminatory information with greater certainty. For the image data, an air-dried salt stressed bacterial sample, discussed in another paper by us in this issue, was studied. Exploration of the image datasets with and without normalisation prior to multivariate analysis by PCA or MAF resulted in different regions of the image being highlighted by the techniques.
Chemical characterization of combustion deposits by TOF-SIMS
NASA Astrophysics Data System (ADS)
Sjövall, P.; Lausmaa, J.; Tullin, C.; Högberg, J.
2003-01-01
We have investigated the potential usefulness of TOF-SIMS for chemical analysis of deposits formed in combustion reactors. By using TOF-SIMS, it was possible to (i) identify inorganic chemical compounds in the deposits, (ii) semi-quantitatively estimate the relative concentrations of the main constituents and (iii) obtain images showing the lateral distribution of the main constituents, on the surface and in cross-sections of deposit samples. It was found that the main components in the deposit samples were KCl and K 2SO 4, while K 2CO 3, NaCl, Na 2SO 4, Ca(OH) 2 and CaCl 2 were present in smaller concentrations. In addition, deposits from combustion of recycled wood chips contained considerable amounts of ZnCl 2, PbCl 2, ZnO and PbO. Large variations in the chemical composition were observed for different samples and throughout the cross-section of a single sample. The chlorides, in particular NaCl, were present mainly as particles, while the sulfates were more homogeneously distributed in the deposit. The results from this study show that TOF-SIMS analysis of combustion deposits can contribute significantly to an increased understanding of the formation and growth of deposits in combustion reactors.
CANbus protocol and applications for STAR TOF Control
NASA Astrophysics Data System (ADS)
Schambach, J.; Bridges, L.; Burton, W.; Eppley, G.; Kajimoto, K.; Nussbaum, T.
2011-12-01
A large-area Time-of-Flight (TOF) system based on Multi-gap Resistive Plate Chambers (MRPCs) has recently been installed in the STAR experiment at RHIC. The approximately 23000 detector channels are read out and digitized using custom electronics based on the CERN NINO and HPTDC chips. The data are sent to the experimental data acquisition system (DAQ) using the ALICE fiber optics based Detector Data Link (DDL). The readout system consists of a total of approximately 2100 custom electronics boards mounted directly on 120 TOF trays, as well as four DAQ and trigger interface boards outside the detector that collect data from 30 trays each and send it to DAQ. Control and monitoring of these electronics boards is done using a tiered network of CANbus connections to a control PC. We describe the physical implementation and topology of the CANbus connections and the custom protocol developed for this project. Several command-line tools as well as a Qt4-based graphical tool developed on the host side to facilitate configuration, control, and monitoring of the TOF system are also described.
Performance of TOF powder diffractometers on reactor sources
NASA Astrophysics Data System (ADS)
Peters, Judith; Bleif, Hans-Jürgen; Kali, György; Rosta, Laszlo; Mezei, Ferenc
2006-11-01
In 1998, a prototype of a time-of-flight (TOF) powder diffractometer was built at KFKI in Budapest in collaboration with the Hahn-Meitner-Institut (HMI) in Berlin. At a reactor source the neutron pulses are produced by a chopper system, which allows for shorter pulses than those obtained at pulsed spallation sources in the wavelength range most relevant for diffraction work, i.e. λ⩾0.7 Å. Furthermore, the chopper system provides an ideal symmetric line shape. First results proved the high potential of the approach, namely an excellent resolution of 1-5×10 -3 for Δ d/d was achieved. The prototype is presently rebuilt as a user instrument at the Budapest Neutron Centre. At HMI Berlin a new much more complex TOF powder diffractometer (EXED=extreme environment diffractometer) with higher resolution is under construction. It will benefit from variable resolution to achieve either ultrahigh resolution or very high intensities at conventional resolutions. EXED is devoted to studies under extreme sample conditions, for instance the TOF technique permits the access of a broad range of Q-values or d-spacing domains under scattering angle access strongly restricted by the use of highest field magnets. The whole instrument was simulated by Monte Carlo (MC) technique, and the simulations yield promising results.
Quantitative Analysis with Heavy Ion E-TOF ERD
Banks, J.C.; Doyle, B.L.; Font, A. Climent
1999-07-23
Heavy ion TOF ERD combined with energy detection (E-TOF-ERD) is a powerful analytical technique taking advantage of the following facts: the scattering cross section is usually very high ({approximately}10{sup {minus}21} cm{sup 2}/sr) compared to regular He RBS ({approximately}10{sup {minus}25} cm{sup 2}/sr), contrary to what happens with the energy resolution in ordinary surface solid barrier detectors, time resolution is almost independent of the atomic mass of the detected element, and the detection in coincidence of time and energy signals allows for the mass separation of overlapping signals with the same energy (or time of flight). Measurements on several oxides have been performed with the E-TOF-ERD set up at Sandia National Laboratories using an incident beam of 10-15 MeV Au. The information on the composition of the sample is obtained from the time domain spectrum, which is converted to energy domain, and then, using existing software codes, the analysis is performed. During the quantification of the results, they have found problems related to the interaction of the beam with the sample and to the tabulated values of the stopping powers for heavy ions.
Spontaneous-Desorption Ionizer for a TOF-MS
NASA Technical Reports Server (NTRS)
Schultz, J. Albert
2006-01-01
A time-of-flight mass spectrometer (TOF-MS) like the one mentioned in the immediately preceding article has been retrofitted with an ionizer based on a surface spontaneous-desorption process. This ionizer includes an electron multiplier in the form of a microchannel plate (MCP). Relative to an ionizer based on a hot-filament electron source, this ionizer offers advantages of less power consumption and greater mechanical ruggedness. The current density and stability characteristics of the electron emission of this ionizer are similar to those of a filament-based ionizer. In tests of various versions of this ionizer in the TOF-MS, electron currents up to 100 nA were registered. Currents of microamperes or more - great enough to satisfy requirements in most TOFMS applications - could be obtained by use of MCPs different from those used in the tests, albeit at the cost of greater bulk. One drawback of this ionizer is that the gain of the MCP decreases as a function of the charge extracted thus far; the total charge that can be extracted over the operational lifetime is about 1 coulomb. An MCP in the ion-detector portion of the TOF-MS is subject to the same limitation.
Perturbations around the zeros of classical orthogonal polynomials
NASA Astrophysics Data System (ADS)
Sasaki, Ryu
2015-04-01
Starting from degree N solutions of a time dependent Schrödinger-like equation for classical orthogonal polynomials, a linear matrix equation describing perturbations around the N zeros of the polynomial is derived. The matrix has remarkable Diophantine properties. Its eigenvalues are independent of the zeros. The corresponding eigenvectors provide the representations of the lower degree ( 0 , 1 , … , N - 1 ) polynomials in terms of the zeros of the degree N polynomial. The results are valid universally for all the classical orthogonal polynomials, including the Askey scheme of hypergeometric orthogonal polynomials and its q-analogues.
Subjective ranking of concert halls substantiated through orthogonal objective parameters.
Cerdá, Salvador; Giménez, Alicia; Cibrián, Rosa; Girón, Sara; Zamarreño, Teófilo
2015-02-01
This paper studies the global subjective assessment, obtained from mean values of the results of surveys addressed to members of the audience of live concerts in Spanish auditoriums, through the mean values of the three orthogonal objective parameters (Tmid, IACCE3, and LEV), expressed in just noticeable differences (JNDs), regarding the best-valued hall. Results show that a linear combination of the relative variations of orthogonal parameters can largely explain the overall perceived quality of the sample. However, the mean values of certain orthogonal parameters are not representative, which shows that an alternative approach to the problem is necessary. Various possibilities are proposed. PMID:25697992
Technology Transfer Automated Retrieval System (TEKTRAN)
Matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF-TOF) mass spectrometry is increasingly utilized for rapid top-down proteomic identification of proteins. This identification may involve analysis of either a pure protein or a protein mixture. For analysis of a pure protein...
A new calibrant for MALDI-TOF-TOF-PSD-MS/MS of non-digested proteins for top-down proteomic analysis
Technology Transfer Automated Retrieval System (TEKTRAN)
RATIONALE: Matrix-assisted laser desorption/ionization (MALDI) time-of-flight-time-of-flight (TOF-TOF) tandem mass spectrometry (MS/MS) has seen increasing use for post-source decay (PSD)-MS/MS analysis of non-digested protein ions for top-down proteomic identification. However, there is no commonl...
Technology Transfer Automated Retrieval System (TEKTRAN)
We have identified six protein biomarkers from two strains of E. coli O157:H7 and one non-pathogenic E. coli strain by matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight tandem mass spectrometry (TOF/TOF-MS/MS) and top-down proteomics. Mature, intact proteins were ext...
Dernovics, Mihaly; García-Barrera, Tamara; Bierla, Katarzyna; Preud'homme, Hugues; Lobinski, Ryszard
2007-05-01
A three-step chromatographic procedure using orthogonal separation mechanisms (size-exclusion, cation-exchange and ion-pairing reversed phase) was developed to purify three low molecular weight selenospecies, including the major compound, from the aqueous extract of monkeypot (Lecythis minor) nuts. The following reversed-phase nanoHPLC-electrospray Q-TOF-MS/MS allowed the formal standardless identification of selenocystathionine and two isoforms of gamma-glutamyl-selenocystathionine. This is the first MS and MS/MS-based formal evidence of the presence of these compounds in a biological sample. PMID:17471390
NASA Astrophysics Data System (ADS)
Dimov, S. S.; Chryssoulis, S. L.
2004-06-01
Results from a comparative study on the detection of organic collectors by TOF-SIMS, vacuum ultraviolet surface analysis by laser ionization with TOF-SIMS detection (VUV SALI TOF-SIMS) and VUV SALI with laser desorption (VUV TOF-LIMS) are reported. The study was carried out on a PHI 7200 TOF-SIMS instrument upgraded with two lasers: one for laser desorption and another one for VUV laser postionization. A systematic analysis of the laser desorption process lead to a set of optimized desorption parameters and desorption of molecules with a controlled level of fragmentation. The recorded spectra of organic collectors by VUV SALI with laser desorption are characterized by strong parent peaks and simpler fragmentation patterns, which allow for easy molecular identification. Advantages and limitations of the three techniques for analysis of organic collectors on mineral grains are discussed.
Analysis of orthogonal waveform for spaceborne MIMO-GMTI radar
NASA Astrophysics Data System (ADS)
Zou, Bo; Dong, Zhen; Du, Xiang-yu
2011-10-01
The application of MIMO (Multiple input multiple output) techniques to spaceborne multichannel radar offers a number of advantages, including target detection, parameter estimation, and so on. Based on two kinds of waveforms presented in MIMO radar, a concise definition of synthetical ISLR is proposed. Through analysis of synthetical ISLR for two kinds of waveforms, it concludes that compared with orthogonal frequency division waveform, the crosscorrelation of orthogonal code waveform badly weakens the performance of spaceborne MIMO radar in GMTI (Ground moving target indication). Thus, by adopting orthogonal frequency division waveform, the basic principle of space-time-frequency adaptive processing is studied. Simulation results demonstrate the superiority of frequency division orthogonal MIMO radar in improving clutter suppression and GMTI performance.
Systems of Differential Equations with Skew-Symmetric, Orthogonal Matrices
ERIC Educational Resources Information Center
Glaister, P.
2008-01-01
The solution of a system of linear, inhomogeneous differential equations is discussed. The particular class considered is where the coefficient matrix is skew-symmetric and orthogonal, and where the forcing terms are sinusoidal. More general matrices are also considered.
Special paraunitary matrices, Cayley transform, and multidimensional orthogonal filter banks.
Zhou, Jianping; Do, Minh N; Kovaĉević, Jelena
2006-02-01
We characterize and design multidimensional (MD) orthogonal filter banks using special paraunitary matrices and the Cayley transform. Orthogonal filter banks are represented by paraunitary matrices in the polyphase domain. We define special paraunitary matrices as paraunitary matrices with unit determinant. We show that every paraunitary matrix can be characterized by a special paraunitary matrix and a phase factor. Therefore, the design of paraunitary matrices (and thus of orthogonal filter banks) becomes the design of special paraunitary matrices, which requires a smaller set of nonlinear equations. Moreover, we provide a complete characterization of special paraunitary matrices in the Cayley domain, which converts nonlinear constraints into linear constraints. Our method greatly simplifies the design of MD orthogonal filter banks and leads to complete characterizations of such filter banks. PMID:16479821
Direct Orthogonal Distance to Quadratic Surfaces in 3D.
Lott, Gus K
2014-09-01
Discovering the orthogonal distance to a quadratic surface is a classic geometric task in vision, modeling, and robotics. I describe a simple, efficient, and stable direct solution for the orthogonal distance (foot-point) to an arbitrary quadratic surface from a general finite 3D point. The problem is expressed as the intersection of three quadratic surfaces, two of which are derived from the requirement of orthogonality of two non-coincident planes with the tangent plane to the quadric. A sixth order single-variable polynomial is directly generated in one coordinate of the surface point. The method detects intersection points at infinity and operates smoothly across all real quadratic surface classes. The method also geometrically detects continuums of orthogonal points (i.e., from the exact center of a sphere). I discuss algorithm performance, compare it to a state-of-the-art estimator, demonstrate the algorithm on synthetic data, and describe extension to arbitrary dimension. PMID:26352239
13. Credit JTL: Detail, orthogonal view of Egyptian Revivial decorative ...
13. Credit JTL: Detail, orthogonal view of Egyptian Revivial decorative motifs used typically at midpoints of diagonals - Reading-Halls Station Bridge, U.S. Route 220, spanning railroad near Halls Station, Muncy, Lycoming County, PA
A proof of van Aubel's theorem using orthogonal vectors
NASA Astrophysics Data System (ADS)
Glaister, P.
2016-04-01
We show how two linearly independent vectors can be used to construct two orthogonal vectors of equal magnitude in a simple way. The proof that the constructed vectors are orthogonal and of equal magnitude is a good exercise for students studying properties of scalar and vector triple products. We then show how this result can be used to prove van Aubel's theorem that relates the two line segments joining the centres of squares on opposite sides of a plane quadrilateral.
Expansion and orthogonalization of measured modes for structure identification
NASA Technical Reports Server (NTRS)
Smith, Suzanne Weaver
1989-01-01
The purpose was to investigate a new simultaneous expansion/orthogonalization method in comparison with two previously published expansion methods and a widely used orthogonalization technique. Each expansion method uses data from an analytical model of the structure to complete the estimate of the mode shape vectors. Berman and Nagy used Guyan expansion in their work with improving analytical models. In this method, modes are expanded one at a time, producing a set not orthogonal with respect to the mass matrix. Baruch and Bar Itzhack's optimal orthogonalization procedure was used to subsequently adjust the expanded modes. A second expansion technique was presented by O'Callahan, Avitabile, and Reimer and separately by Kammer. Again, modes are expanded individually and orthogonalized after expansion with the same optimal technique as above. Finally, a simultaneous expansion/orthogonalization method was developed from the orthogonal Procrustes problem of computational mathematics. In this method modes are optimally expanded as a set and orthogonal with respect to the mass matrix as a result. Two demonstation problems were selected for the comparison of the methods described. The first problem is an 8 degree of freedom spring-mass problem first presented by Kabe. Several conditions were examined for expansion method including the presence of errors in the measured data and in the analysis models. As a second demonstration problem, data from tests of laboratory scale model truss structures was expanded for system identification. Tests with a complete structure produced a correlated analysis model and the stiffness and mass matrices. Tests of various damaged configurations produced measured data for 6 modes at 14 dof locations.
Local unitary equivalence of quantum states and simultaneous orthogonal equivalence
NASA Astrophysics Data System (ADS)
Jing, Naihuan; Yang, Min; Zhao, Hui
2016-06-01
The correspondence between local unitary equivalence of bipartite quantum states and simultaneous orthogonal equivalence is thoroughly investigated and strengthened. It is proved that local unitary equivalence can be studied through simultaneous similarity under projective orthogonal transformations, and four parametrization independent algorithms are proposed to judge when two density matrices on ℂd1 ⊗ ℂd2 are locally unitary equivalent in connection with trace identities, Kronecker pencils, Albert determinants and Smith normal forms.
... injection is used to treat wet age-related macular degeneration (AMD; an ongoing disease of the eye that causes loss of the ability to see straight ahead and may make it more ... used to treat macular edema after retinal vein occlusion (an eye disease ...
... prednisone to treat prostate cancer (cancer of a male reproductive organ) that has already been treated with other medications. Cabazitaxel injection is in a class of medications called microtubule inhibitors. It works by slowing or stopping the growth of cancer cells.
... purple blotches or lines under the skin skin depressions at the injection site increased body fat or movement to different areas of your body difficulty falling asleep or staying asleep inappropriate happiness extreme ... increased sweating muscle weakness joint pain dizziness irregular ...
... purple blotches or lines under the skin skin depressions at the injection site increased body fat or movement to different areas of your body difficulty falling asleep or staying asleep inappropriate happiness extreme ... increased sweating muscle weakness joint pain dizziness irregular ...
... a sudden wave of mild or intense body heat) decreased sexual ability or desire leg or joint pain breast pain pain, itching, swelling, or redness at the place where injection was given difficulty falling asleep or staying asleep Some side effects can be serious. If you experience any of ...
... tests to check your body's response to eribulin injection.It is important for you to keep a written list of all of the prescription and nonprescription (over-the-counter) medicines you are taking, as well as any products such as vitamins, minerals, or other dietary supplements. You should bring ...
... to 7 days after you receive each pegaptanib injection.It is important for you to keep a written list of all of the prescription and nonprescription (over-the-counter) medicines you are taking, as well as any products such as vitamins, minerals, or other dietary supplements. You should bring ...
... and pharmacist what other prescription and nonprescription medications, vitamins, nutritional supplements, and herbal products you are taking or plan to take. Be sure to mention any of the following: allergy shots (a series of injections given regularly to prevent the body from developing ...
... treat ulcers (sores in the lining of the stomach or intestine) that were not successfully treated with other medications. ... Ellison syndrome (tumors in the pancreas and small intestine that caused increased production of stomach acid). Famotidine injection is in a class of ...
... treat ulcers (sores in the lining of the stomach or intestine) that were not successfully treated with other medications. ... Ellison syndrome (tumors in the pancreas and small intestine that caused increased production of stomach acid). Ranitidine injection is in a class of ...
... provider immediately: chest pain or difficulty breathing confusion fast or irregular heartbeat severe headache irritation at the injection site If you experience a serious side effect, you or your doctor may send a report to the Food and Drug Administration's (FDA) MedWatch Adverse Event Reporting ...
... will be given to you two times a day for 2 to 3 weeks, and then once a day, 5 to 7 days of each week.Your dose of ganciclovir will ... may give you several doses (enough for a day's supply) of premixed ganciclovir injection solution at one ...
... have received ibritumomab injection.do not have any vaccinations without talking to your doctor.you should know ... cells) and myelodysplastic syndrome (condition in which blood cells do not ... online (http://www.fda.gov/Safety/MedWatch) or by phone (1-800-332-1088).
... have received tositumomab injection.do not have any vaccinations without talking to your doctor.you should know ... blood cells), myelodysplastic syndrome (condition in which blood cells do not ... online (http://www.fda.gov/Safety/MedWatch) or by phone (1-800-332-1088).
... other body tissues where it is used for energy. Dulaglutide injection also works by slowing the movement ... In case of overdose, call your local poison control center at 1-800-222-1222. If the victim has collapsed or is not breathing, call local emergency services at ...
... other body tissues where it is used for energy. Liraglutide injection also slows the emptying of the ... In case of overdose, call your local poison control center at 1-800-222-1222. If the victim has collapsed or is not breathing, call local emergency services at ...
... other body tissues where it is used for energy. Albiglutide injection also works by slowing the movement ... In case of overdose, call your local poison control center at 1-800-222-1222. If the victim has collapsed or is not breathing, call local emergency services at ...
Orthogonal metals: The simplest non-Fermi liquids
NASA Astrophysics Data System (ADS)
Nandkishore, Rahul; Metlitski, Max A.; Senthil, T.
2012-07-01
We present a fractionalized metallic phase which is indistinguishable from the Fermi liquid in conductivity and thermodynamics, but is sharply distinct in one-electron properties, such as the electron spectral function. We dub this phase the “orthogonal metal.” The orthogonal metal and the transition to it from the Fermi liquid are naturally described using a slave-particle representation wherein the electron is expressed as a product of a fermion and a slave Ising spin. We emphasize that when the slave spins are disordered, the result is not a Mott insulator (as erroneously assumed in the prior literature), but rather the orthogonal metal. We construct prototypical ground-state wave functions for the orthogonal metal by modifying the Jastrow factor of Slater-Jastrow wave functions that describe ordinary Fermi liquids. We further demonstrate that the transition from the Fermi liquid to the orthogonal metal can, in some circumstances, provide a simple example of a continuous destruction of a Fermi surface with a critical Fermi surface appearing right at the critical point. We present exactly soluble models that realize an orthogonal metal phase, and the phase transition to the Fermi liquid. These models thus provide valuable solvable examples for phase transitions associated with the death of a Fermi surface.
Expeditious oligosaccharide synthesis via selective, semi-orthogonal, and orthogonal activation
Kaeothip, Sophon; Demchenko, Alexei V.
2011-01-01
Traditional strategies for oligosaccharide synthesis often require extensive protecting and/or leaving group manipulations between each glycosylation step, thereby increasing the total number of synthetic steps while decreasing the efficiency of the synthesis. In contrast, expeditious strategies allow for the rapid chemical synthesis of complex carbohydrates by minimizing extraneous chemical manipulations. Oligosaccharide synthesis by selective activation of one leaving group over another is one such expeditious strategy. Herein, the significant improvements that have recently emerged in the area of the selective activation are discussed. The development of orthogonal strategy further expands the scope of the selective activation methodology. Surveyed in this article, are representative examples wherein these excellent innovations have been applied to the synthesis of various oligosaccharide sequences. PMID:21663897
Choi, Minseok; Sapsis, Themistoklis P.; Karniadakis, George Em
2014-08-01
The Karhunen–Lòeve (KL) decomposition provides a low-dimensional representation for random fields as it is optimal in the mean square sense. Although for many stochastic systems of practical interest, described by stochastic partial differential equations (SPDEs), solutions possess this low-dimensional character, they also have a strongly time-dependent form and to this end a fixed-in-time basis may not describe the solution in an efficient way. Motivated by this limitation of standard KL expansion, Sapsis and Lermusiaux (2009) [26] developed the dynamically orthogonal (DO) field equations which allow for the simultaneous evolution of both the spatial basis where uncertainty ‘lives’ but also the stochastic characteristics of uncertainty. Recently, Cheng et al. (2013) [28] introduced an alternative approach, the bi-orthogonal (BO) method, which performs the exact same tasks, i.e. it evolves the spatial basis and the stochastic characteristics of uncertainty. In the current work we examine the relation of the two approaches and we prove theoretically and illustrate numerically their equivalence, in the sense that one method is an exact reformulation of the other. We show this by deriving a linear and invertible transformation matrix described by a matrix differential equation that connects the BO and the DO solutions. We also examine a pathology of the BO equations that occurs when two eigenvalues of the solution cross, resulting in an instantaneous, infinite-speed, internal rotation of the computed spatial basis. We demonstrate that despite the instantaneous duration of the singularity this has important implications on the numerical performance of the BO approach. On the other hand, it is observed that the BO is more stable in nonlinear problems involving a relatively large number of modes. Several examples, linear and nonlinear, are presented to illustrate the DO and BO methods as well as their equivalence.
Large strip RPCs for the LEPS2 TOF system
NASA Astrophysics Data System (ADS)
Tomida, N.; Niiyama, M.; Ohnishi, H.; Tran, N.; Hsieh, C.-Y.; Chu, M.-L.; Chang, W.-C.; Chen, J.-Y.
2014-12-01
High time-resolution resistive plate chambers (RPCs) with large-size readout strips are developed for the time-of-flight (TOF) detector system of the LEPS2 experiment at SPring-8. The experimental requirement is a 50-ps time resolution for a strip size larger than 100 cm2/channel. We are able to achieve 50-ps time resolutions with 2.5×100 cm2 strips by directly connecting the amplifiers to strips. With the same time resolution, the number of front-end electronics (FEE) is also reduced by signal addition.
[Studies of bacterial typing with MALDI-TOF].
Culebras, Esther; Alvarez-Buylla, Adela; Jose Artacho Reinoso, M; Antonio Lepe, Jose
2016-06-01
MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry has emerged as a potential tool for microbial characterization and identification in many microbiology departments. The technology is rapid, sensitive, and relatively inexpensive in terms of both the labour and costs involved. This review provides an overview on its utility for strain typing and epidemiological studies and explains the methodological approaches that can be used both for the performance of the technique and for the analysis of results. Finally, the review summarizes studies on the characterization of distinct bacterial species. PMID:27389292
Method for improving the time resolution of a TOF system
NASA Astrophysics Data System (ADS)
Li, Shao-Li; Heng, Yue-Kun; Zhao, Tian-Chi; Fu, Zai-Wei; Liu, Shu-Lin; Qian, Sen; Liu, Shu-Dong; Chen, Xiao-Hui; Jia, Ru; Huang, Guo-Rui; Lei, Xiang-Cui
2013-01-01
In order to study the possibility of improving the timing performance of the time of flight (TOF) systems, which are made of plastic scintillator counters, and read out by photomultiplier tubes (PMT) with mesh dynodes and conventional electronics, we have conducted a study using faster PMTs and ultra fast waveform digitizers to read out the plastic scintillators. Different waveform analysis methods are used to calculate the time resolution of such a system. Results are compared with the conventional discriminating method based on a threshold and pulse height. Our tests and analysis show that significant timing performance improvements can be achieved by using this new system.
MONSTER: a TOF Spectrometer for β-delayed Neutron Spectroscopy
Martínez, T.; Cano-Ott, D.; Castilla, J.; Garcia, A.R.; Marin, J.; Martinez, G.; Mendoza, E.; Santos, C.; Tera, F.J.; Villamarin, D.; Agramunt, J.; Algora, A.; Domingo, C.; Jordan, M.D.; Rubio, B.; Taín, J.L.; Bhattacharya, C.; Banerjee, K.; Bhattacharya, S.; Roy, P.; and others
2014-06-15
β-delayed neutron (DN) data, including emission probabilities, Pn, and energy spectrum, play an important role in our understanding of nuclear structure, nuclear astrophysics and nuclear technologies. A MOdular Neutron time-of-flight SpectromeTER (MONSTER) is being built for the measurement of the neutron energy spectra and branching ratios. The TOF spectrometer will consist of one hundred liquid scintillator cells covering a significant solid angle. The MONSTER design has been optimized by using Monte Carlo (MC) techniques. The response function of the MONSTER cell has been characterized with mono-energetic neutron beams and compared to dedicated MC simulations.
Chang, Kai Lun; Ho, Paul C
2014-01-01
Findings from epidemiology, preclinical and clinical studies indicate that consumption of coffee could have beneficial effects against dementia and Alzheimer's disease (AD). The benefits appear to come from caffeinated coffee, but not decaffeinated coffee or pure caffeine itself. Therefore, the objective of this study was to use metabolomics approach to delineate the discriminant metabolites between caffeinated and decaffeinated coffee, which could have contributed to the observed therapeutic benefits. Gas chromatography time-of-flight mass spectrometry (GC-TOF-MS)-based metabolomics approach was employed to characterize the metabolic differences between caffeinated and decaffeinated coffee. Orthogonal partial least squares discriminant analysis (OPLS-DA) showed distinct separation between the two types of coffee (cumulative Q(2) = 0.998). A total of 69 discriminant metabolites were identified based on the OPLS-DA model, with 37 and 32 metabolites detected to be higher in caffeinated and decaffeinated coffee, respectively. These metabolites include several benzoate and cinnamate-derived phenolic compounds, organic acids, sugar, fatty acids, and amino acids. Our study successfully established GC-TOF-MS based metabolomics approach as a highly robust tool in discriminant analysis between caffeinated and decaffeinated coffee samples. Discriminant metabolites identified in this study are biologically relevant and provide valuable insights into therapeutic research of coffee against AD. Our data also hint at possible involvement of gut microbial metabolism to enhance therapeutic potential of coffee components, which represents an interesting area for future research. PMID:25098597
Steiner, Wes E; Harden, Charles S; Hong, Feng; Klopsch, Steve J; Hill, Herbert H; McHugh, Vincent M
2006-02-01
The use of negative ion monitoring mode with an atmospheric pressure ion mobility orthogonal reflector time-of-flight mass spectrometer [IM(tof)MS] to detect chemical warfare agent (CWA) degradation products from aqueous phase samples has been determined. Aqueous phase sampling used a traditional electrospray ionization (ESI) source for sample introduction and ionization. Certified reference materials (CRM) of CWA degradation products for the detection of Schedule 1, 2, or 3 toxic chemicals or their precursors as defined by the chemical warfare convention (CWC) treaty verification were used in this study. A mixture of six G-series nerve related CWA degradation products (EMPA, IMPA, EHEP, IHEP, CHMPA, and PMPA) and their related collision induced dissociation (CID) fragment ions (MPA and EPA) were found in each case to be clearly resolved and detected using the IM(tof)MS instrument in negative ion monitoring mode. Corresponding ions, masses, drift times, K(o) values, and signal intensities for each of the CWA degradation products are reported. PMID:16413205
Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA synthetase pairs
Schultz, Peter G.; Wang, Lei; Anderson, John Christopher; Chin, Jason W.; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Stephen William; Zhang, Zhiwen
2011-09-06
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
Schultz, Peter G.; Wang, Lei; Anderson, John Christopher; Chin, Jason; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Steven William; Zhang, Zhiwen
2012-05-22
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
Schultz, Peter G.; Wang, Lei; Anderson, John Christopher; Chin, Jason; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Steven William; Zhang, Zhiwen
2008-04-08
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
Schultz, Peter G.; Wang, Lei; Anderson, John Christopher; Chin, Jason; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Stephen William; Zhang, Zhiwen
2010-05-11
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
A modular strategy for engineering orthogonal chimeric RNA transcription regulators
Takahashi, Melissa K.; Lucks, Julius B.
2013-01-01
Antisense RNA transcription attenuators are a key component of the synthetic biology toolbox, with their ability to serve as building blocks for both signal integration logic circuits and transcriptional cascades. However, a central challenge to building more sophisticated RNA genetic circuitry is creating larger families of orthogonal attenuators that function independently of each other. Here, we overcome this challenge by developing a modular strategy to create chimeric fusions between the engineered transcriptional attenuator from plasmid pT181 and natural antisense RNA translational regulators. Using in vivo gene expression assays in Escherichia coli, we demonstrate our ability to create chimeric attenuators by fusing sequences from five different translational regulators. Mutagenesis of these functional attenuators allowed us to create a total of 11 new chimeric attenutaors. A comprehensive orthogonality test of these culminated in a 7 × 7 matrix of mutually orthogonal regulators. A comparison between all chimeras tested led to design principles that will facilitate further engineering of orthogonal RNA transcription regulators, and may help elucidate general principles of non-coding RNA regulation. We anticipate that our strategy will accelerate the development of even larger families of orthogonal RNA transcription regulators, and thus create breakthroughs in our ability to construct increasingly sophisticated RNA genetic circuitry. PMID:23761434
Neutron Capture Measurements at the n lowbar TOF Facility
Milazzo, P. M.; Abbondanno, U.; Fujii, K.; Aerts, G.; Andriamonje, S.; Berthoumieux, E.; Dridi, W.; Ferrant, L.; Gunsing, F.; Pancin, J.; Perrot, L.; Plukis, A.; Stephan, C.; Tassan-Got, L.; Alvarez-Velarde, F.; Cano-Ott, D.; Embid-Segura, M.
2009-03-31
Nuclear astrophysics, advanced nuclear technology and nuclear structure physics present many cases that require neutron capture reaction data with high precision. In particular, refined data are needed for stellar nucleosynthesis, for nuclear waste transmutation studies, and for the design of generation IV reactors. The measurements take profit of the pulsed neutron beam of the n lowbar TOF facility at CERN, which is generated by proton-induced spallation reactions on a massive lead target. The low repetition rate of the proton beam, the high instantaneous neutron flux, and the favourable background conditions in the experimental area make this facility unique for high resolution time-of-flight measurements of neutron induced reaction cross sections. The n lowbar TOF collaboration is presently operating two different experimental set-ups. The first consists of two low-neutron sensitivity C{sub 6}D{sub 6} detectors with the analysis relying on the Pulse Height Weighting technique. In addition, a Total Absorption Calorimeter, consisting of 40 BaF{sub 2} crystals covering the whole solid angle, was used. A review of several capture measurements with these detectors on selected stable and unstable samples will be presented.
Rapid identification of Streptomyces isolates by MALDI-TOF MS.
Loucif, Lotfi; Bendjama, Esma; Gacemi-Kirane, Djamila; Rolain, Jean-Marc
2014-12-01
The recent emergence of multidrug-resistant bacteria over the last decade has led to a renewal in the discovery of new antimicrobial drugs. Streptomyces members are practically unlimited sources of new antibiotics. However, the identification of Streptomyces species is difficult and time-consuming. Therefore, there is a need for alternative methods for their rapid identification. In this study, an efficient protocol of identification using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) was developed and applied for the rapid identification of Streptomyces isolates from the El Kala lakes in northeastern Algeria. A collection of 48 Streptomyces isolates were used for this study. The optimized procedure allowed us to obtain specific and reproducible protein spectra for each Streptomyces isolate tested. The spectra generated were used to build a preliminary local database based on their initial 16S rRNA identification. The blind test used for the identification of 20 Streptomyces strains already available in our created database and 20 unknown Streptomyces isolates showed that all (100%) of the Streptomyces strains listed in the database were rapidly (<30min) identified with high scores of up to 2.8. Here, for the first time we showed that MALDI-TOF MS could be used as a cost-effective tool for the rapid identification of Streptomyces isolates. PMID:24862894
Challenges in biomarker discovery with MALDI-TOF MS.
Hajduk, Joanna; Matysiak, Jan; Kokot, Zenon J
2016-07-01
MALDI-TOF MS technique is commonly used in system biology and clinical studies to search for new potential markers associated with pathological conditions. Despite numerous concerns regarding a sample preparation or processing of complex data, this strategy is still recognized as a popular tool and its awareness has risen in the proteomic community over the last decade. In this review, we present comprehensive application of MALDI mass spectrometry with special focus on profiling research. We also discuss major advantages and disadvantages of universal sample preparation methods such as micro-SPE columns, immunodepletion or magnetic beads, and we show the potential of nanostructured materials in capturing low molecular weight subproteomes. Furthermore, as the general protocol considerably affects spectra quality and interpretation, an alternative solution for improved ion detection, including hydrophobic constituents, data processing and statistical analysis is being considered in up-to-date profiling pattern. In conclusion, many reports involving MALDI-TOF MS indicated highly abundant proteins as valuable indicators, and at the same time showed the inaccuracy of available methods in the detection of low abundant proteome that is the most interesting from the clinical perspective. Therefore, the analytical aspects of sample preparation methods should be standardized to provide a reproducible, low sample handling and credible procedure. PMID:27134187
Carrier-interleaved orthogonal multi-electrode multi-carrier resistivity-measurement tool
NASA Astrophysics Data System (ADS)
Cai, Yu; Sha, Shuang
2016-09-01
This paper proposes a new carrier-interleaved orthogonal multi-electrode multi-carrier resistivity-measurement tool used in a cylindrical borehole environment during oil-based mud drilling processes. The new tool is an orthogonal frequency division multiplexing access-based contactless multi-measurand detection tool. The tool can measure formation resistivity in different azimuthal angles and elevational depths. It can measure many more measurands simultaneously in a specified bandwidth than the legacy frequency division multiplexing multi-measurand tool without a channel-select filter while avoiding inter-carrier interference. The paper also shows that formation resistivity is not sensitive to frequency in certain frequency bands. The average resistivity collected from N subcarriers can increase the measurement of the signal-to-noise ratio (SNR) by N times given no amplitude clipping in the current-injection electrode. If the clipping limit is taken into account, with the phase rotation of each single carrier, the amplitude peak-to-average ratio can be reduced by 3 times, and the SNR can achieve a 9/N times gain over the single-carrier system. The carrier-interleaving technique is also introduced to counter the carrier frequency offset (CFO) effect, where the CFO will cause inter-pad interference. A qualitative analysis and simulations demonstrate that block-interleaving performs better than tone-interleaving when coping with a large CFO. The theoretical analysis also suggests that increasing the subcarrier number can increase the measurement speed or enhance elevational resolution without sacrificing receiver performance. The complex orthogonal multi-pad multi-carrier resistivity logging tool, in which all subcarriers are complex signals, can provide a larger available subcarrier pool than other types of transceivers.
Ion composition in interchange injection events in Saturn's magnetosphere
NASA Astrophysics Data System (ADS)
Thomsen, M. F.; Reisenfeld, D. B.; Wilson, R. J.; Andriopoulou, M.; Crary, F. J.; Hospodarsky, G. B.; Jackman, C. M.; Jia, X.; Khurana, K. K.; Paranicas, C.; Roussos, E.; Sergis, N.; Tokar, R. L.
2014-12-01
Interchange injection events are commonly observed by the Cassini spacecraft in the region between about 6 and 12 Rs (1 Rs = 60,268 km) and even frequently beyond. In this study, 13 examples of interchange injection events are identified in Cassini/Cassini Plasma Spectrometer data under special conditions such that time-of-flight (TOF) mass spectra could be obtained from entirely within the events. Using the TOF data to separate the main ion species H+, H2+, and W+, approximate densities of each species are calculated under the assumption that all distributions were isotropic. The light-ion density ratios, H2+/H+, in the injection events are not discernibly different from those ratios in control intervals from the ambient plasma. However, the water-group ratio, W+/H+, is significantly lower than ambient. The comparison of the measured density ratios with the range of values observed throughout Saturn's magnetosphere indicates that the values of W+/H+ that are as low as those observed within the injection events are found primarily beyond L~14 (where L is the equatorial crossing distance, in Saturn radius, of a dipole field line), indicating that the injection events are delivering plasma from the outer magnetosphere at times traveling at least 6 Rs.
Locally indistinguishable orthogonal product bases in arbitrary bipartite quantum system
NASA Astrophysics Data System (ADS)
Xu, Guang-Bao; Yang, Ying-Hui; Wen, Qiao-Yan; Qin, Su-Juan; Gao, Fei
2016-08-01
As we know, unextendible product basis (UPB) is an incomplete basis whose members cannot be perfectly distinguished by local operations and classical communication. However, very little is known about those incomplete and locally indistinguishable product bases that are not UPBs. In this paper, we first construct a series of orthogonal product bases that are completable but not locally distinguishable in a general m ⊗ n (m ≥ 3 and n ≥ 3) quantum system. In particular, we give so far the smallest number of locally indistinguishable states of a completable orthogonal product basis in arbitrary quantum systems. Furthermore, we construct a series of small and locally indistinguishable orthogonal product bases in m ⊗ n (m ≥ 3 and n ≥ 3). All the results lead to a better understanding of the structures of locally indistinguishable product bases in arbitrary bipartite quantum system.
Locally indistinguishable orthogonal product bases in arbitrary bipartite quantum system.
Xu, Guang-Bao; Yang, Ying-Hui; Wen, Qiao-Yan; Qin, Su-Juan; Gao, Fei
2016-01-01
As we know, unextendible product basis (UPB) is an incomplete basis whose members cannot be perfectly distinguished by local operations and classical communication. However, very little is known about those incomplete and locally indistinguishable product bases that are not UPBs. In this paper, we first construct a series of orthogonal product bases that are completable but not locally distinguishable in a general m ⊗ n (m ≥ 3 and n ≥ 3) quantum system. In particular, we give so far the smallest number of locally indistinguishable states of a completable orthogonal product basis in arbitrary quantum systems. Furthermore, we construct a series of small and locally indistinguishable orthogonal product bases in m ⊗ n (m ≥ 3 and n ≥ 3). All the results lead to a better understanding of the structures of locally indistinguishable product bases in arbitrary bipartite quantum system. PMID:27503634
Investigation of formation mechanisms of chips in orthogonal cutting process
NASA Astrophysics Data System (ADS)
Ma, W.
2012-08-01
This work investigates the formation mechanisms of chips in orthogonal cutting of mild steel and the transformation conditions between various morphology chips. It is supposed that the modeling material follows the Johnson-Cook constitutive model. In orthogonal cutting process, both the plastic flow and the instability behaviors of chip materials are caused by the plane strain loadings. Therefore, the general instability behaviors of materials in plane strain state are first analyzed with linear perturbation method and a universal instability criterion is established. Based on the analytical results, the formation mechanisms of chips and the transformation conditions between continuous and serrated chips are further studied by instability phase diagram method. The results show that the chip formation strongly depends on the intensity ratios between shear and normal stresses. The ratios of dissipative rates of plastic work done by compression and shear stresses govern the transformation from continuous to serrated chips. These results are verified by the numerical simulations on the orthogonal cutting process.
Bio-orthogonally Deciphered Binary Nanoemitters for Tumor Diagnostics.
An, Hong-Wei; Qiao, Sheng-Lin; Li, Li-Li; Yang, Chao; Lin, Yao-Xin; Wang, Yi; Qiao, Zeng-Ying; Wang, Lei; Wang, Hao
2016-08-01
Bioinspired design concept has been recognized as one of the most promising strategies for discovering new biomaterials. However, smart biomaterials that are of growing interests in biomedical field need biological processability to meet their emergent applications in vivo. Herein, a new bio-orthogonally deciphered approach has been demonstrated for modulating optical properties of nanomaterials in living systems. The self-assembled nanoemitters based on cyanine-pyrene molecule 1 with inert optical property are designed and prepared. The structure and optical feature of the nanoemitters 1 can be efficiently and reliably modulated by a unique bio-orthogonal mechanism with abundant glutathione (GSH) as an activator. As a result, the self-assembled nanoemitters 1 spontaneously exhibits binary emissions for high-performance tumor imaging in vivo. We believe that this bio-orthogonally deciphered strategy opens a new avenue for designing variable smart biomaterials or devices in biomedical applications. PMID:27434548
Orthogonal chemical functionalization of patterned gold on silica surfaces
Léonard, Didier; Le Mogne, Thierry; Zuttion, Francesca; Chevalier, Céline; Phaner-Goutorbe, Magali; Souteyrand, Éliane
2015-01-01
Summary Single-step orthogonal chemical functionalization procedures have been developed with patterned gold on silica surfaces. Different combinations of a silane and a thiol were simultaneously deposited on a gold/silica heterogeneous substrate. The orthogonality of the functionalization (i.e., selective grafting of the thiol on the gold areas and the silane on the silica) was demonstrated by X-ray photoelectron spectroscopy (XPS) as well as time-of-flight secondary ion mass spectrometry (ToF–SIMS) mapping. The orthogonal functionalization was used to immobilize proteins onto gold nanostructures on a silica substrate, as demonstrated by atomic force microscopy (AFM). These results are especially promising in the development of future biosensors where the selective anchoring of target molecules onto nanostructured transducers (e.g., nanoplasmonic biosensors) is a major challenge. PMID:26734519
Orthogonal chemical functionalization of patterned gold on silica surfaces.
Palazon, Francisco; Léonard, Didier; Le Mogne, Thierry; Zuttion, Francesca; Chevalier, Céline; Phaner-Goutorbe, Magali; Souteyrand, Éliane; Chevolot, Yann; Cloarec, Jean-Pierre
2015-01-01
Single-step orthogonal chemical functionalization procedures have been developed with patterned gold on silica surfaces. Different combinations of a silane and a thiol were simultaneously deposited on a gold/silica heterogeneous substrate. The orthogonality of the functionalization (i.e., selective grafting of the thiol on the gold areas and the silane on the silica) was demonstrated by X-ray photoelectron spectroscopy (XPS) as well as time-of-flight secondary ion mass spectrometry (ToF-SIMS) mapping. The orthogonal functionalization was used to immobilize proteins onto gold nanostructures on a silica substrate, as demonstrated by atomic force microscopy (AFM). These results are especially promising in the development of future biosensors where the selective anchoring of target molecules onto nanostructured transducers (e.g., nanoplasmonic biosensors) is a major challenge. PMID:26734519
Orthogonal test and experimental study on fire floating pump
NASA Astrophysics Data System (ADS)
Liu, J. R.; Zheng, J. F.; Fu, D. P.; Wang, P.
2013-12-01
In order to develop high efficiency fire floating pump, 250YYB-250 fire floating pump was taken as an example. The orthogonal experiment of L9 (34), which contains factors with three levels of blade numbers of impeller, outlet angle, impeller fold-angle, was performed to design nine types of impellers. Numerical simulation of whole flow field based on Fluent was adopted to perform an orthogonal test, the order of geometric parameters affects the performance of fire floating pump with complex impeller. The best design scheme for pump model was acquired. Meanwhile, the optimized design scheme was determined, and corresponding test was carried out. It demonstrated that the efficiency of the final optimal design model pump at rated flow point is of 85%. The efficiency is higher than the national standards, which verified the feasibility of the method of orthogonal design in pump design.
Scattering equations and Kawai-Lewellen-Tye orthogonality
NASA Astrophysics Data System (ADS)
Cachazo, Freddy; He, Song; Yuan, Ellis Ye
2014-09-01
Several recent developments point to the fact that rational maps from n-punctured spheres to the null cone of D-dimensional momentum space provide a natural language for describing the scattering of massless particles in D dimensions. In this paper we identify and study equations relating the kinematic invariants sab and the puncture locations σc, which we call the scattering equations. We provide an inductive algorithm in the number of particles for their solutions and prove a remarkable property which we call Kawai-Lewellen-Tye (KLT) orthogonality. In a nutshell, KLT orthogonality means that "Parke-Taylor" vectors constructed from the solutions to the scattering equations are mutually orthogonal with respect to the KLT bilinear form. We end with comments on possible connections to gauge theory and gravity amplitudes in any dimension and to the high-energy limit of string theory amplitudes.
Experimental quantum-cryptography scheme based on orthogonal states
NASA Astrophysics Data System (ADS)
Avella, Alessio; Brida, Giorgio; Degiovanni, Ivo Pietro; Genovese, Marco; Gramegna, Marco; Traina, Paolo
2010-12-01
Since, in general, nonorthogonal states cannot be cloned, any eavesdropping attempt in a quantum-communication scheme using nonorthogonal states as carriers of information introduces some errors in the transmission, leading to the possibility of detecting the spy. Usually, orthogonal states are not used in quantum-cryptography schemes since they can be faithfully cloned without altering the transmitted data. Nevertheless, L. Goldberg and L. Vaidman [Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.75.1239 75, 1239 (1995)] proposed a protocol in which, even if the data exchange is realized using two orthogonal states, any attempt to eavesdrop is detectable by the legal users. In this scheme the orthogonal states are superpositions of two localized wave packets traveling along separate channels. Here we present an experiment realizing this scheme.
Calculation on flux-MMF relationship of orthogonal-core
Tajima, K.; Kaga, A.; Anazawa, Y. ); Ichinokura, O. )
1993-03-01
Orthogonal-cores have various potential applications, for instance in parametric transformers and dc-ac converters. The operating characteristics of the devices can be calculated on the basis of the measured relationship of flux to MMF of the orthogonal-core. To achieve optimal design of the applied device, the relationship of flux to MMF must be determined; however, this involves solving a three dimensional nonlinear problem. In this paper, the authors calculate the flux-MMF relationship based on a magnetic circuit model for the orthogonal-core. The computed results agree well with experiment. The method of this study is shown to be valid for calculation of characteristics and useful for optimal design of application devices.
Semi-orthogonal wavelets for elliptic variational problems
Hardin, D.P.; Roach, D.W.
1998-04-01
In this paper the authors give a construction of wavelets which are (a) semi-orthogonal with respect to an arbitrary elliptic bilinear form a({center_dot},{center_dot}) on the Sobolev space H{sub 0}{sup 1}((0, L)) and (b) continuous and piecewise linear on an arbitrary partition of [0, L]. They illustrate this construction using a model problem. They also construct alpha-orthogonal Battle-Lemarie type wavelets which fully diagonalize the Galerkin discretized matrix for the model problem with domain IR. Finally they describe a hybrid basis consisting of a combination of elements from the semi-orthogonal wavelet basis and the hierarchical Schauder basis. Numerical experiments indicate that this basis leads to robust scalable Galerkin discretizations of the model problem which remain well-conditioned independent of {epsilon}, L, and the refinement level K.
Experimental quantum-cryptography scheme based on orthogonal states
Avella, Alessio; Brida, Giorgio; Degiovanni, Ivo Pietro; Genovese, Marco; Gramegna, Marco; Traina, Paolo
2010-12-15
Since, in general, nonorthogonal states cannot be cloned, any eavesdropping attempt in a quantum-communication scheme using nonorthogonal states as carriers of information introduces some errors in the transmission, leading to the possibility of detecting the spy. Usually, orthogonal states are not used in quantum-cryptography schemes since they can be faithfully cloned without altering the transmitted data. Nevertheless, L. Goldberg and L. Vaidman [Phys. Rev. Lett. 75, 1239 (1995)] proposed a protocol in which, even if the data exchange is realized using two orthogonal states, any attempt to eavesdrop is detectable by the legal users. In this scheme the orthogonal states are superpositions of two localized wave packets traveling along separate channels. Here we present an experiment realizing this scheme.
Locally indistinguishable orthogonal product bases in arbitrary bipartite quantum system
Xu, Guang-Bao; Yang, Ying-Hui; Wen, Qiao-Yan; Qin, Su-Juan; Gao, Fei
2016-01-01
As we know, unextendible product basis (UPB) is an incomplete basis whose members cannot be perfectly distinguished by local operations and classical communication. However, very little is known about those incomplete and locally indistinguishable product bases that are not UPBs. In this paper, we first construct a series of orthogonal product bases that are completable but not locally distinguishable in a general m ⊗ n (m ≥ 3 and n ≥ 3) quantum system. In particular, we give so far the smallest number of locally indistinguishable states of a completable orthogonal product basis in arbitrary quantum systems. Furthermore, we construct a series of small and locally indistinguishable orthogonal product bases in m ⊗ n (m ≥ 3 and n ≥ 3). All the results lead to a better understanding of the structures of locally indistinguishable product bases in arbitrary bipartite quantum system. PMID:27503634
Measurement matrix optimization method based on matrix orthogonal similarity transformation
NASA Astrophysics Data System (ADS)
Pan, Jinfeng
2016-05-01
Optimization of the measurement matrix is one of the important research aspects of compressive sensing theory. A measurement matrix optimization method is presented based on the orthogonal similarity transformation of the information operator's Gram matrix. In terms of the fact that the information operator's Gram matrix is a singular symmetric matrix, a simplified orthogonal similarity transformation is deduced, and thus the simplified diagonal matrix that is orthogonally similar to it is obtained. Then an approximation of the Gram matrix is obtained by letting all the nonzero diagonal entries of the simplified diagonal matrix equal their average value. Thus an optimized measurement matrix can be acquired according to its relationship with the information operator. Results of experiments show that the optimized measurement matrix compared to the random measurement matrix is less coherent with dictionaries. The relative signal recovery error also declines when the proposed measurement matrix is utilized.
Mayer, Brian P; DeHope, Alan J; Mew, Daniel A; Spackman, Paul E; Williams, Audrey M
2016-04-19
Attribution of the origin of an illicit drug relies on identification of compounds indicative of its clandestine production and is a key component of many modern forensic investigations. The results of these studies can yield detailed information on method of manufacture, starting material source, and final product, all critical forensic evidence. In the present work, chemical attribution signatures (CAS) associated with the synthesis of the analgesic fentanyl, N-(1-phenylethylpiperidin-4-yl)-N-phenylpropanamide, were investigated. Six synthesis methods, all previously published fentanyl synthetic routes or hybrid versions thereof, were studied in an effort to identify and classify route-specific signatures. A total of 160 distinct compounds and inorganic species were identified using gas and liquid chromatographies combined with mass spectrometric methods (gas chromatography/mass spectrometry (GC/MS) and liquid chromatography-tandem mass spectrometry-time of-flight (LC-MS/MS-TOF)) in conjunction with inductively coupled plasma mass spectrometry (ICPMS). The complexity of the resultant data matrix urged the use of multivariate statistical analysis. Using partial least-squares-discriminant analysis (PLS-DA), 87 route-specific CAS were classified and a statistical model capable of predicting the method of fentanyl synthesis was validated and tested against CAS profiles from crude fentanyl products deposited and later extracted from two operationally relevant surfaces: stainless steel and vinyl tile. This work provides the most detailed fentanyl CAS investigation to date by using orthogonal mass spectral data to identify CAS of forensic significance for illicit drug detection, profiling, and attribution. PMID:27010913
Wang, Fei; Chen, Fang-Fang; Gao, Wen-Bo; Wang, Hai-Yong; Zhao, Ning-Wei; Xu, Min; Gao, De-Yu; Yu, Wei; Yan, Xiao-Ling; Zhao, Jian-Ning; Li, Xiao-Jun
2016-09-01
The objective of the study is to investigate potential citrullinated autoantigens as targets of anti-citrullinated protein antibodies (ACPAs) response in synovial fluids (SFs) of patients with rheumatoid arthritis (RA). SFs from six RA patients and six osteoarthritis (OA) patients as controls were collected. The citrullinated proteins in SFs were extracted by immunoprecipitation with rabbit anti-citrulline antibodies. Matrix-assisted laser desorption/ionization time of flight mass spectrometry/time of flight mass spectrometry (MALDI-TOF/TOF) mass spectrometry was subsequently performed to discover a characteristic neutral loss to finally determine citrullinated autoantigens. A total of 182 citrullinated peptides and 200 citrullinated sites were identified in RA SFs, while 3 citrullinated peptides and 4 citrullinated sites were identified in OA SFs. The 182 citrullinated peptides from RA SFs and the 3 citrullinated peptides from OA SFs were derived from 83 and 3 autoantigens, respectively. Eighty-three autoantigens except protein-arginine deiminase type-2 (PADI2) and protein-arginine deiminase type-2 (PADI4) were over-citrullinated compared with controls, and the citrullinated sites of PADI2 and PADI4 were different in two groups. Interestingly, citrullinated histone H3.3 (H3F3A) was found in OA controls, but not in RA groups. The differential citrullinated proteins identified in RA SFs suggested potential autoantigens were targeted for ACPAs response and might contribute to the induction and perpetuation of complement activation and joint inflammation in RA. PMID:27060082
Luczak, Magdalena; Marczak, Lukasz; Stobiecki, Maciej
2014-01-01
Shotgun proteomic methods involving iTRAQ (isobaric tags for relative and absolute quantitation) peptide labeling facilitate quantitative analyses of proteomes and searches for useful biomarkers. However, the plasma proteome's complexity and the highly dynamic plasma protein concentration range limit the ability of conventional approaches to analyze and identify a large number of proteins, including useful biomarkers. The goal of this paper is to elucidate the best approach for plasma sample pretreatment for MS- and iTRAQ-based analyses. Here, we systematically compared four approaches, which include centrifugal ultrafiltration, SCX chromatography with fractionation, affinity depletion, and plasma without fractionation, to reduce plasma sample complexity. We generated an optimized protocol for quantitative protein analysis using iTRAQ reagents and an UltrafleXtreme (Bruker Daltonics) MALDI TOF/TOF mass spectrometer. Moreover, we used a simple, rapid, efficient, but inexpensive sample pretreatment technique that generated an optimal opportunity for biomarker discovery. We discuss the results from the four sample pretreatment approaches and conclude that SCX chromatography without affinity depletion is the best plasma sample preparation pretreatment method for proteome analysis. Using this technique, we identified 1,780 unique proteins, including 1,427 that were quantified by iTRAQ with high reproducibility and accuracy. PMID:24988083
Wang, Da-Zhi; Li, Cheng; Xie, Zhang-Xian; Dong, Hong-Po; Lin, Lin; Hong, Hua-Sheng
2011-01-01
This study developed a multilayered, gel-based, and underivatized strategy for de novo protein sequence analysis of unsequenced dinoflagellates using a MALDI-TOF/TOF mass spectrometer with the assistance of DeNovo Explorer software. MASCOT was applied as the first layer screen to identify either known or unknown proteins sharing identical peptides presented in a database. Once the confident identifications were removed after searching against the NCBInr database, the remainder was searched against the dinoflagellate expressed sequence tag database. In the last layer, those borderline and nonconfident hits were further subjected to de novo interpretation using DeNovo Explorer software. The de novo sequences passing a reliability filter were subsequently submitted to nonredundant MS-BLAST search. Using this layer identification method, 216 protein spots representing 158 unique proteins out of 220 selected protein spots from Alexandrium tamarense, a dinoflagellate with unsequenced genome, were confidently or tentatively identified by database searching. These proteins were involved in various intracellular physiological activities. This study is the first effort to develop a completely automated approach to identify proteins from unsequenced dinoflagellate databases and establishes a preliminary protein database for various physiological studies of dinoflagellates in the future. PMID:21977052
Tandem time-of-flight mass spectrometer (TOF-TOF) with a quadratic-field ion mirror
NASA Astrophysics Data System (ADS)
Giannakopulos, Anastassios E.; Thomas, Benjamin; Colburn, Alex W.; Reynolds, David J.; Raptakis, Emmanuel N.; Makarov, Alexander A.; Derrick, Peter J.
2002-05-01
A tandem time-of-flight (TOF-TOF) mass spectrometer comprised of two ion mirrors is described. The first ion mirror, which is a linear-field, single-stage mirror (MS1) with an intermediate collision cell, has been designed to provide the temporal focus necessary for the second, quadratic-field ion mirror (MS2) to function effectively. Due to the wide energy-range focusing capabilities of the quadratic field employed in the second ion mirror all the fragment ions can be collected in one spectrum without the need to step the reflecting working voltage of the MS2. The size of the active area of the microchannel plate detector used in the preliminary experiments was the limiting factor governing the collection efficiently of fragment ions. The use of the first ion mirror to provide temporal focusing of the precursor ion packet at the first focal point of the quadratic mirror used as the MS2 requires no alteration of the focusing conditions for different masses, in contrast to delayed extraction or postsource pulsed focusing. Precursor ions formed by matrix-assisted laser desorption/ionization were mass-selected with an ion gate located before the collision cell and the fragment ions were mass analyzed using the quadratic-field ion mirror. Experimental results demonstrating effective high-energy collision-induced dissociation of polymer and fullerene molecule-ions are presented.
MALDI-TOF MS of Trichoderma: A model system for the identification of microfungi
Technology Transfer Automated Retrieval System (TEKTRAN)
This investigation aimed to assess whether MALDI-TOF MS analysis of proteomics could be applied to the study of Trichoderma, a fungal genus selected because it includes many species and is phylogenetically well defined. We also investigated whether MALDI-TOF MS analysis of proteomics would reveal ap...
TOF-SIMS Analysis of Red Color Inks of Writing and Printing Tools on Questioned Documents.
Lee, Jihye; Nam, Yun Sik; Min, Jisook; Lee, Kang-Bong; Lee, Yeonhee
2016-05-01
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is a well-established surface technique that provides both elemental and molecular information from several monolayers of a sample surface while also allowing depth profiling or image mapping to be performed. Static TOF-SIMS with improved performances has expanded the application of TOF-SIMS to the study of a variety of organic, polymeric, biological, archaeological, and forensic materials. In forensic investigation, the use of a minimal sample for the analysis is preferable. Although the TOF-SIMS technique is destructive, the probing beams have microsized diameters so that only small portion of the questioned sample is necessary for the analysis, leaving the rest available for other analyses. In this study, TOF-SIMS and attenuated total reflectance Fourier transform infrared (ATR-FTIR) were applied to the analysis of several different pen inks, red sealing inks, and printed patterns on paper. The overlapping areas of ballpoint pen writing, red seal stamping, and laser printing in a document were investigated to identify the sequence of recording. The sequence relations for various cases were determined from the TOF-SIMS mapping image and the depth profile. TOF-SIMS images were also used to investigate numbers or characters altered with two different red pens. TOF-SIMS was successfully used to determine the sequence of intersecting lines and the forged numbers on the paper. PMID:27122425
Connection preserving deformations and q-semi-classical orthogonal polynomials
NASA Astrophysics Data System (ADS)
Ormerod, Christopher M.; Witte, N. S.; Forrester, Peter J.
2011-09-01
We present a framework for the study of q-difference equations satisfied by q-semi-classical orthogonal systems. As an example, we identify the q-difference equation satisfied by a deformed version of the little q-Jacobi polynomials as a gauge transformation of a special case of the associated linear problem for q-PVI. We obtain a parametrization of the associated linear problem in terms of orthogonal polynomial variables and find the relation between this parametrization and that of Jimbo and Sakai.
Universal Continuous-Variable State Orthogonalizer and Qubit Generator
NASA Astrophysics Data System (ADS)
Coelho, Antonio S.; Costanzo, Luca S.; Zavatta, Alessandro; Hughes, Catherine; Kim, M. S.; Bellini, Marco
2016-03-01
We experimentally demonstrate a universal strategy for producing a quantum state that is orthogonal to an arbitrary, infinite-dimensional, pure input one, even if only a limited amount of information about the latter is available. Arbitrary coherent superpositions of the two mutually orthogonal states are then produced by a simple change in the experimental parameters. We use input coherent states of light to illustrate two variations of the method. However, we show that the scheme works equally well for arbitrary input fields and constitutes a universal procedure, which may thus prove a useful building block for quantum state engineering and quantum information processing with continuous-variable qubits.
Equipment and preliminary results for orthogonal pressurized planar electrochromatography.
Dzido, Tadeusz H; Lopaciuk, Eryk; Płocharz, Paweł W; Chomicki, Adam; Zembrzycka, Magdalena; Frank, Hartmut
2014-03-21
We report combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) techniques into a single technique in which both OPLC and PPEC processes proceed simultaneously and orthogonally. The separation process with this new technique is performed in adsorbent layer of a chromatographic plate, which is equipped with special sealing margin on its whole periphery and closed under pressure in special chamber. We have named this separation technique as orthogonal pressurized planar electrochromatography (OPPEC). Examples of analytical and micropreparative (continuous) OPPEC separations are demonstrated. PMID:24572544
National Ignition Facility (NIF) Neutron time-of-flight (nTOF) Measurements
Lerche, R A; Glebov, V Y; Moran, M J; McNaney, J M; Kilkenny, J D; Eckart, M; Zacharias, R A; Haslam, J J; Clancy, T J; Yeoman, M F; Warwas, D P; Sangster, T C; Stoeckl, C; Knauer, J; Horsfield, C J
2010-05-13
The first three of eighteen neutron time-of-flight (nTOF) channels have been installed at the National Ignition Facility (NIF). The role of these detectors includes yield, temperature, and bang time measurements. This article focuses on nTOF data analysis and quality of results obtained for the first set of experiments to use all 192 NIF beams. Targets produced up to 2 x 10{sup 10} 2.45-MeV neutrons for initial testing of the nTOF detectors. Differences in neutron scattering at the OMEGA laser facility where the detectors were calibrated and at NIF result in different response functions at the two facilities. Monte Carlo modeling shows this difference. The nTOF performance on these early experiments indicates the nTOF system with its full complement of detectors should perform well in future measurements of yield, temperature, and bang time.
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Study of a Cherenkov TOF-PET module
NASA Astrophysics Data System (ADS)
Korpar, S.; Dolenec, R.; Križan, P.; Pestotnik, R.; Stanovnik, A.
2013-12-01
An apparatus, consisting of two PbF2 crystals, each coupled to a multichannel plate photomultiplier (MCP-PMT), has been constructed in order to measure the time-of-flight (TOF) of the two 511 keV annihilation photons produced in positron emission tomography (PET). Excellent timing is achieved by detecting the prompt Cherenkov photons produced by the absorption of the 511 keV gamma photons. The present work describes the measurement and image reconstruction of two 22Na point sources. In addition, the influence of the radiator thickness and the Cherenkov light absorption cut-off of the crystal on the efficiency and the timing resolution have been studied by Monte Carlo simulation.
TOF-SIMS investigation of Streptomyces coelicolor, a mycelial bacterium
NASA Astrophysics Data System (ADS)
Vaidyanathan, Seetharaman; Fletcher, John S.; Lockyer, Nicholas P.; Vickerman, John C.
2008-12-01
Streptomyces coelicolor is a mycelial microorganism that produces several secondary metabolites, including antibiotics. The physiology of the organism has largely been investigated in liquid cultures due to ease of monitoring different physiological parameters and more homogeneous culture conditions. However, solid cultures reflect the natural physiology of the microorganism better, given that in its natural state it grows in the soil. Imaging mass spectrometry with TOF-SIMS and C 60+ primary ion beams offers a potential route to studying chemical changes at the molecular level, both intracellular and extracellular that can help in understanding the natural physiology of the microorganism. Here, we report the application of the technique for studying the lateral distribution of the chemical species detected in a population, grown in both liquid and solid cultures. The capability of the technique for studying biological systems with minimal system intervention is demonstrated.
A MRPC prototype for SOLID-TOF in Jlab
Wang, Y; Wang, J; Gonzalez-Diaz, D; Chen, H; Chen, J; Li, Y; Camsonne, A; Chen, J -P; Gao, H
2013-03-01
A prototype of Multi-gap Resistive Plate Chamber (MRPC) for the future SoLID time of flight system at JLab has been developed. The counter, trapezoidal in shape, is assembled with the newly developed low-resistive Chinese glass. It has 10 × 0.25 mm gas gaps and 11 readout strips of different lengths. The strip width is 2.5 cm with a strip-to-strip interval of 3 mm. Preliminary tests performed with cosmic-rays showed an efficiency higher than 95% and a time resolution around 50 ps. Results under diffuse/uniform irradiation performed at JLab with scattered high energy electrons showed a time resolution of 70–80 ps and over-95% efficiency up to an incoming flux of 15 kHz/cm{sup 2}. These performances meet the requirements of the new time of flight system SoLID-TOF.
Analysis of TOF-SIMS spectra from fullerene compounds
NASA Astrophysics Data System (ADS)
Kato, N.; Yamashita, Y.; Iida, S.; Sanada, N.; Kudo, M.
2008-12-01
We analyzed TOF-SIMS spectra obtained from three different size of fullerenes (C 60, C 70 and C 84) by using Ga +, Au + and Au 3+ primary ion beams and investigated the fragmentation patterns, the enhancement of secondary ion yields and the restraint of fragmentation by using cluster primary ion beams compared with monoatomic primary ion beams. In the TOS-SIMS spectra from C 70 and C 84, it was found that a fragment ion, identified as C 60+ ( m/ z = 720), showed a relatively high intensity compared with that of other fragment ions related to C 2 depletion. It was also found that the Au 3+ bombardment caused intensity enhancement of intact molecules (C 60+, C 70+ and C 84+) and restrained the fragmentation due to C 2 depletion.
Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry
Parker, Laurie; Engel-Hall, Aaron; Drew, Kevin; Steinhardt, George; Helseth, Donald L.; Jabon, David; McMurry, Timothy; Angulo, David S.; Kron, Stephen J.
2010-01-01
Despite advances in methods and instrumentation for analysis of phosphopeptides using mass spectrometry, it is still difficult to quantify the extent of phosphorylation of a substrate due to physiochemical differences between unphosphorylated and phosphorylated peptides. Here we report experiments to investigate those differences using MALDI-TOF mass spectrometry for a set of synthetic peptides by creating calibration curves of known input ratios of peptides/phosphopeptides and analyzing their resulting signal intensity ratios. These calibration curves reveal subtleties in sequence-dependent differences for relative desorption/ionization efficiencies that cannot be seen from single-point calibrations. We found that the behaviors were reproducible with a variability of 5–10% for observed phosphopeptide signal. Although these data allow us to begin addressing the issues related to modeling these properties and predicting relative signal strengths for other peptide sequences, it is clear this behavior is highly complex and needs to be further explored. PMID:18064576
Microorganism Identification Based On MALDI-TOF-MS Fingerprints
NASA Astrophysics Data System (ADS)
Elssner, Thomas; Kostrzewa, Markus; Maier, Thomas; Kruppa, Gary
Advances in MALDI-TOF mass spectrometry have enabled the development of a rapid, accurate and specific method for the identification of bacteria directly from colonies picked from culture plates, which we have named the MALDI Biotyper. The picked colonies are placed on a target plate, a drop of matrix solution is added, and a pattern of protein molecular weights and intensities, "the protein fingerprint" of the bacteria, is produced by the MALDI-TOF mass spectrometer. The obtained protein mass fingerprint representing a molecular signature of the microorganism is then matched against a database containing a library of previously measured protein mass fingerprints, and scores for the match to every library entry are produced. An ID is obtained if a score is returned over a pre-set threshold. The sensitivity of the techniques is such that only approximately 104 bacterial cells are needed, meaning that an overnight culture is sufficient, and the results are obtained in minutes after culture. The improvement in time to result over biochemical methods, and the capability to perform a non-targeted identification of bacteria and spores, potentially makes this method suitable for use in the detect-to-treat timeframe in a bioterrorism event. In the case of white-powder samples, the infectious spore is present in sufficient quantity in the powder so that the MALDI Biotyper result can be obtained directly from the white powder, without the need for culture. While spores produce very different patterns from the vegetative colonies of the corresponding bacteria, this problem is overcome by simply including protein fingerprints of the spores in the library. Results on spores can be returned within minutes, making the method suitable for use in the "detect-to-protect" timeframe.
Digital orthogonal receiver for wideband radar based on compressed sensing
NASA Astrophysics Data System (ADS)
Hou, Qingkai; Liu, Yang; Chen, Zengping; Su, Shaoying
2014-10-01
Digital orthogonal receiver is one of the key techniques in digital receiver of soft radar, and compressed sensing is attracting more and more attention in radar signal processing. In this paper, we propose a CS digital orthogonal receiver for wideband radar which utilizes compressed sampling in the acquisition of radar raw data. In order to reconstruct complex signal from sub-sampled raw data, a novel sparse dictionary is proposed to represent the real-valued radar raw signal sparsely. Using our dictionary and CS algorithm, we can reconstruct the complex-valued radar signal from sub-sampled echoes. Compared with conventional digital orthogonal radar receiver, the architecture of receiver in this paper is more simplified and the sampling frequency of ADC is reduced sharply. At the same time, the range profile can be obtained during the reconstruction, so the matched filtering can be eliminated in the receiver. Some experiments on ISAR imaging based on simulated data prove that the phase information of radar echoes is well reserved in our orthogonal receiver and the whole design is effective for wideband radar.
Response Surface Model Building Using Orthogonal Arrays for Computer Experiments
NASA Technical Reports Server (NTRS)
Unal, Resit; Braun, Robert D.; Moore, Arlene A.; Lepsch, Roger A.
1997-01-01
This study investigates response surface methods for computer experiments and discusses some of the approaches available. Orthogonal arrays constructed for computer experiments are studied and an example application to a technology selection and optimization study for a reusable launch vehicle is presented.
Crossover ensembles of random matrices and skew-orthogonal polynomials
Kumar, Santosh; Pandey, Akhilesh
2011-08-15
Highlights: > We study crossover ensembles of Jacobi family of random matrices. > We consider correlations for orthogonal-unitary and symplectic-unitary crossovers. > We use the method of skew-orthogonal polynomials and quaternion determinants. > We prove universality of spectral correlations in crossover ensembles. > We discuss applications to quantum conductance and communication theory problems. - Abstract: In a recent paper (S. Kumar, A. Pandey, Phys. Rev. E, 79, 2009, p. 026211) we considered Jacobi family (including Laguerre and Gaussian cases) of random matrix ensembles and reported exact solutions of crossover problems involving time-reversal symmetry breaking. In the present paper we give details of the work. We start with Dyson's Brownian motion description of random matrix ensembles and obtain universal hierarchic relations among the unfolded correlation functions. For arbitrary dimensions we derive the joint probability density (jpd) of eigenvalues for all transitions leading to unitary ensembles as equilibrium ensembles. We focus on the orthogonal-unitary and symplectic-unitary crossovers and give generic expressions for jpd of eigenvalues, two-point kernels and n-level correlation functions. This involves generalization of the theory of skew-orthogonal polynomials to crossover ensembles. We also consider crossovers in the circular ensembles to show the generality of our method. In the large dimensionality limit, correlations in spectra with arbitrary initial density are shown to be universal when expressed in terms of a rescaled symmetry breaking parameter. Applications of our crossover results to communication theory and quantum conductance problems are also briefly discussed.
Orthogonal Projection in Teaching Regression and Financial Mathematics
ERIC Educational Resources Information Center
Kachapova, Farida; Kachapov, Ilias
2010-01-01
Two improvements in teaching linear regression are suggested. The first is to include the population regression model at the beginning of the topic. The second is to use a geometric approach: to interpret the regression estimate as an orthogonal projection and the estimation error as the distance (which is minimized by the projection). Linear…
Trusted materials using orthogonal testing. 2015 Annual report
Van Benthem, Mark
2015-09-01
The purpose of this project is to prove (or disprove) that a reasonable number of simple tests can be used to provide a unique data signature for materials, changes in which could serve as a harbinger of material deviation, prompting further evaluations. The routine tests are mutually orthogonal to any currently required materials specification tests.
Distortion correction for the orthogonally-splitting-imaging pose sensor
NASA Astrophysics Data System (ADS)
Yang, Qian; Sun, Chang-ku; Wang, Peng; Li, Wen-qiang; Liu, Xin-tong
2015-06-01
The orthogonally-splitting-imaging pose sensor utilizes not only large field of view spherical lenses but also two sets of cylindrical ones to realize the high-speed, high-precision and wide-field pose measurement. Notable distortion, however, results from the wide-field lenses at the same time. Therefore, to obtain the best performance of the camera model, a distortion correction method is proposed in this paper, which combines the advantages of the high-stability of the Least Square fittings based on the orthogonal polynomials and the independence of the distortion correction based on the cross-ratio invariability. In this way, the ill-conditioned fitting matrix as well as the iteration and optimization procedures in solving extrinsic and intrinsic parameters can be avoided. Due to the wide fitness of the cross-ratio invariability and the orthogonal polynomials, this distortion correction technique is also suitable to other optical set up with different imaging structure. The experiment results that the corrected grids have superior precision and reliability with their original slopes demonstrate that the distortion model on the basis of orthogonal polynomial is validated and that the distortion correction is effective.
Complex Exceptional Orthogonal Polynomials and Quasi-invariance
NASA Astrophysics Data System (ADS)
Haese-Hill, William A.; Hallnäs, Martin A.; Veselov, Alexander P.
2016-05-01
Consider the Wronskians of the classical Hermite polynomials H_{λ, l}(x):=Wr(H_l(x),H_{k_1}(x),ldots,H_{k_n}(x)), quad l in Z_{≥0}{setminus} {k_1,ldots,k_n}, where {k_i=λ_i+n-i, i=1,ldots, n} and {λ=(λ_1, ldots, λ_n)} is a partition. Gómez-Ullate et al. showed that for a special class of partitions the corresponding polynomials are orthogonal and dense among all polynomials with respect to a certain inner product, but in contrast to the usual case have some degrees missing (so-called exceptional orthogonal polynomials). We generalise their results to all partitions by considering complex contours of integration and non-positive Hermitian products. The corresponding polynomials are orthogonal and dense in a finite-codimensional subspace of {C[x]} satisfying certain quasi-invariance conditions. A Laurent version of exceptional orthogonal polynomials, related to monodromy-free trigonometric Schrödinger operators, is also presented.
Alternative Kondo breakdown mechanism: Orbital-selective orthogonal metal transition
NASA Astrophysics Data System (ADS)
Zhong, Yin; Liu, Ke; Wang, Yong-Qiang; Luo, Hong-Gang
2012-09-01
In a recent paper of Nandkishore, Metlitski, and Senthil [Phys. Rev. B1098-012110.1103/PhysRevB.86.045128 86, 045128 (2012)], a concept of orthogonal metal has been introduced to reinterpret the disordered state of slave-spin representation in the Hubbard model as an exotic gapped metallic state. We extend this concept to study the corresponding quantum phase transition in the extended Anderson lattice model. It is found that the disordered state of slave spins in this model is an orbital-selective orthogonal metal, a generalization of the concept of the orthogonal metal in the Hubbard model. The quantum critical behaviors are multiscale and dominated by a z=3 and z=2 critical modes in the high- and low-temperature regime, respectively. Such behaviors are obviously in contrast to the naive expectation in the Hubbard model. The result provides alternative Kondo breakdown mechanism for heavy fermion compounds underlying the physics of the orbital-selective orthogonal metal in the disordered state, which is different from the conventional Kondo breakdown mechanism with the fractionalized Fermi-liquid picture. This work is expected to be useful in understanding the quantum criticality happening in some heavy fermion materials and other related strongly correlated systems.
A Monotonically Convergent Algorithm for Orthogonal Congruence Rotation.
ERIC Educational Resources Information Center
Kiers, Henk A. L.; Groenen, Patrick
1996-01-01
An iterative majorization algorithm is proposed for orthogonal congruence rotation that is guaranteed to converge from every starting point. In addition, the algorithm is easier to program than the algorithm proposed by F. B. Brokken, which is not guaranteed to converge. The derivation of the algorithm is traced in detail. (SLD)
Chemical Bonding: The Orthogonal Valence-Bond View
Sax, Alexander F.
2015-01-01
Chemical bonding is the stabilization of a molecular system by charge- and spin-reorganization processes in chemical reactions. These processes are said to be local, because the number of atoms involved is very small. With multi-configurational self-consistent field (MCSCF) wave functions, these processes can be calculated, but the local information is hidden by the delocalized molecular orbitals (MO) used to construct the wave functions. The transformation of such wave functions into valence bond (VB) wave functions, which are based on localized orbitals, reveals the hidden information; this transformation is called a VB reading of MCSCF wave functions. The two-electron VB wave functions describing the Lewis electron pair that connects two atoms are frequently called covalent or neutral, suggesting that these wave functions describe an electronic situation where two electrons are never located at the same atom; such electronic situations and the wave functions describing them are called ionic. When the distance between two atoms decreases, however, every covalent VB wave function composed of non-orthogonal atomic orbitals changes its character from neutral to ionic. However, this change in the character of conventional VB wave functions is hidden by its mathematical form. Orthogonal VB wave functions composed of orthonormalized orbitals never change their character. When localized fragment orbitals are used instead of atomic orbitals, one can decide which local information is revealed and which remains hidden. In this paper, we analyze four chemical reactions by transforming the MCSCF wave functions into orthogonal VB wave functions; we show how the reactions are influenced by changing the atoms involved or by changing their local symmetry. Using orthogonal instead of non-orthogonal orbitals is not just a technical issue; it also changes the interpretation, revealing the properties of wave functions that remain otherwise undetected. PMID:25906476
Inverse spin Hall effect by spin injection
NASA Astrophysics Data System (ADS)
Liu, S. Y.; Horing, Norman J. M.; Lei, X. L.
2007-09-01
Motivated by a recent experiment [S. O. Valenzuela and M. Tinkham, Nature (London) 442, 176 (2006)], the authors present a quantitative microscopic theory to investigate the inverse spin-Hall effect with spin injection into aluminum considering both intrinsic and extrinsic spin-orbit couplings using the orthogonalized-plane-wave method. Their theoretical results are in good agreement with the experimental data. It is also clear that the magnitude of the anomalous Hall resistivity is mainly due to contributions from extrinsic skew scattering.
NASA Astrophysics Data System (ADS)
Cardamone, Jeanette M.
2010-04-01
Investigations of keratins extracted from wool by reduction hydrolysis and by alkaline hydrolysis showed that their chemical compositions and secondary structures were similar to original wool. The keratin isolates were similar in amino acid, Amides I and II, and secondary structure to each other and to original wool. From SDS-PAGE electrophoresis, keratin isolated by reduction contained protein homologs of molecular weight, ˜40-60 kDa and keratin isolate from alkaline hydrolysis contained peptide fragments of ˜6-8 kDa. MALDI-TOF/TOF spectrometry confirmed that the reduction isolate contained Type II microfibrillar component 7C, hair Type II intermediate filament, Type I microfibrillar 48 kDa component 8C-1, and Type I microfibrillar 47.6 kDa protein homologs which contained alanine, glutamine, glutamic acid, leucine, serine, leucine, and cystine with highest amounts glutamic acid and leucine amino acids. FTIR spectroscopy was applied to examine secondary structure to confirm the content of α-helix/β-sheet/disordered regions for original wool (58.2%/37.9%/3.9%); keratin from reduction (36.7%/50.2%/13.1%); and keratin from alkaline hydrolysis (25.7%/51.8%/22.5%). The higher content of β-sheet secondary structure and intact α-helical conformation characterized these isolates as viable starting materials for chemical modification to form novel bio-based materials useful in industrial formulations and compositions. In particular keratin extracted by reduction with the molecular weight of original wool and the probability of useful mechanical properties can be transformed into stand-alone products of various shapes and forms such as porous foams, sponges, mats, and films for bio-based, adaptable structures.
Identification of Dermatophyte Species after Implementation of the In-House MALDI-TOF MS Database
Calderaro, Adriana; Motta, Federica; Montecchini, Sara; Gorrini, Chiara; Piccolo, Giovanna; Piergianni, Maddalena; Buttrini, Mirko; Medici, Maria Cristina; Arcangeletti, Maria Cristina; Chezzi, Carlo; De Conto, Flora
2014-01-01
Despite that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a powerful tool in the clinical microbiology setting, few studies have till now focused on MALDI-TOF MS-based identification of dermatophytes. In this study, we analyze dermatophytes strains isolated from clinical samples by MALDI-TOF MS to supplement the reference database available in our laboratory. Twenty four dermatophytes (13 reference strains and 11 field isolated strains), identified by both conventional and molecular standard procedures, were analyzed by MALDI-TOF MS, and the spectra obtained were used to supplement the available database, limited to a few species. To verify the robustness of the implemented database, 64 clinical isolates other than those used for the implementation were identified by MALDI-TOF MS. The implementation allowed the identification of the species not included in the original database, reinforced the identification of the species already present and correctly identified those within the Trichophyton mentagrophytes complex previously classified as Trichophyton. tonsurans by MALDI-TOF MS. The dendrogram obtained by analyzing the proteic profiles of the different species of dermatophytes reflected their taxonomy, showing moreover, in some cases, a different clusterization between the spectra already present in the database and those newly added. In this study, MALDI-TOF MS proved to be a useful tool suitable for the identification of dermatophytes for diagnostic purpose. PMID:25216335
Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
Anderson, J Christopher [San Francisco, CA; Schultz, Peter G [La Jolla, CA; Santoro, Stephen [Cambridge, MA
2009-05-05
Compositions and methods of producing components of protein biosynthetic machinery that include glutamyl orthogonal tRNAs, glutamyl orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of glutamyl tRNAs/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
Direct MALDI-TOF mass spectrometric peptide profiling of neuroendocrine tissue of Drosophila.
Wegener, Christian; Neupert, Susanne; Predel, Reinhard
2010-01-01
Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to analyze the peptide complement in the nervous system of a variety of invertebrate animals, from leech to Aplysia and many arthropod species, especially insects and crustaceans. Proper sample preparation is often the most crucial step to obtain the necessary data. Here, we describe protocols for the use of MALDI-TOF mass spectrometry to directly analyze the peptidome of neuroendocrine tissues of insects, particularly Drosophila melanogaster, by MALDI-TOF MS. PMID:20013204
Polarized optical injection in long-wavelength vertical-cavity surface emitting lasers
NASA Astrophysics Data System (ADS)
Hurtado, A.; Schires, K.; Khan, N.; Al-Seyab, R.; Henning, I. D.; Adams, M. J.
2011-05-01
We report a comprehensive study of the effects of polarized optical injection in long-wavelength Vertical-Cavity Surface Emitting Lasers (LW-VCSELs) emitting at the telecom wavelength of 1550nm. We analyze the properties of the polarization switching and bistability that can be induced in a 1550nm-VCSEL under orthogonal and arbitrary polarized optical injection. Additionally, we study the injection locking bandwidth of these devices when subject to different polarized optical injection. Furthermore, we also analyze the relationship existing between the injection locking bandwidth and the polarization switching range when the device is subject to orthogonally-polarized optical injection. Finally, we have identified regions of different nonlinear dynamics outside the injection locking bandwidth, including regions of periodic dynamics (such as limit cycle and period doubling) and chaos when these devices are subject to parallel and to orthogonal optical injection. This rich variety of nonlinear effects observed at 1550nm offers exciting prospects for novel practical uses of VCSELs in optical switching/routing applications in optical networks.
CVS Decomposition of 3D Homogeneous Turbulence Using Orthogonal Wavelets
NASA Technical Reports Server (NTRS)
Farge, Marie; Schneider, Kai; Pellegrino, Giulio; Wray, A. A.; Rogallo, R. S.
2000-01-01
This paper compares the filtering used in Coherent Vortex Simulation (CVS) decomposition with an orthogonal wavelet basis, with the Proper Orthogonal Decomposition (POD) or Fourier filtering. Both methods are applied to a field of Direct Numerical Simulation (DNS) data of 3D forced homogeneous isotropic turbulence at microscale Reynolds number R(sub lambda) = 168. We show that, with only 3%N retained modes, CVS filtering separates the coherent vortex tubes from the incoherent background flow. The latter is structureless, has an equipartition energy spectrum, and has a Gaussian velocity probability distribution function (PDF) and an exponential vorticity PDF. On the other hand, the Fourier basis does not extract the coherent vortex tubes cleanly and leaves organized structures in the residual high wavenumber modes whose PDFs are stretched exponentials for both the velocity and the vorticity.
Orthogonally modulated molecular transport junctions for resettable electronic logic gates
Meng, Fanben; Hervault, Yves-Marie; Shao, Qi; Hu, Benhui; Norel, Lucie; Rigaut, Stéphane; Chen, Xiaodong
2014-01-01
Individual molecules have been demonstrated to exhibit promising applications as functional components in the fabrication of computing nanocircuits. Based on their advantage in chemical tailorability, many molecular devices with advanced electronic functions have been developed, which can be further modulated by the introduction of external stimuli. Here, orthogonally modulated molecular transport junctions are achieved via chemically fabricated nanogaps functionalized with dithienylethene units bearing organometallic ruthenium fragments. The addressable and stepwise control of molecular isomerization can be repeatedly and reversibly completed with a judicious use of the orthogonal optical and electrochemical stimuli to reach the controllable switching of conductivity between two distinct states. These photo-/electro-cooperative nanodevices can be applied as resettable electronic logic gates for Boolean computing, such as a two-input OR and a three-input AND-OR. The proof-of-concept of such logic gates demonstrates the possibility to develop multifunctional molecular devices by rational chemical design. PMID:24394717
Tracking Lung Tumors in Orthogonal X-Rays
2013-01-01
This paper presents a computationally very efficient, robust, automatic tracking method that does not require any implanted fiducials for low-contrast tumors. First, it generates a set of motion hypotheses and computes corresponding feature vectors in local windows within orthogonal-axis X-ray images. Then, it fits a regression model that maps features to 3D tumor motions by minimizing geodesic distances on motion manifold. These hypotheses can be jointly generated in 3D to learn a single 3D regression model or in 2D through back projection to learn two 2D models separately. Tumor is tracked by applying regression to the consecutive image pairs while selecting optimal window size at every time. Evaluations are performed on orthogonal X-ray videos of 10 patients. Comparative experimental results demonstrate superior accuracy (~1 pixel average error) and robustness to varying imaging artifacts and noise at the same time. PMID:23986789
Orthogonal canonical forms for second-order systems
NASA Technical Reports Server (NTRS)
Williams, Trevor; Laub, Alan
1989-01-01
The authors prove that a linear second-order system with arbitrary damping cannot be reduced to Hessenberg-triangular form by means of orthogonal transformations, while this reduction is always possible for the modal damping commonly assumed for models of flexible structures. The type of canonical form obtainable by means of orthogonal transformations acting on a second-order system is heavily dependent on the type of damping considered. If the damping matrix is merely positive semi-definite symmetric, it is generally not possible to obtain a reduction to Hessenberg-triangular form, while this reduction is trivial for zero or Rayleigh damping. If damping is modal, however, as is commonly assumed in structural models, the reduction exists and is nontrivial. Furthermore, reduction to triangular second-order Schur form is always possible for such damping: this canonical form appears likely to have applications to second-order system theory.
Modelling Trends in Ordered Correspondence Analysis Using Orthogonal Polynomials.
Lombardo, Rosaria; Beh, Eric J; Kroonenberg, Pieter M
2016-06-01
The core of the paper consists of the treatment of two special decompositions for correspondence analysis of two-way ordered contingency tables: the bivariate moment decomposition and the hybrid decomposition, both using orthogonal polynomials rather than the commonly used singular vectors. To this end, we will detail and explain the basic characteristics of a particular set of orthogonal polynomials, called Emerson polynomials. It is shown that such polynomials, when used as bases for the row and/or column spaces, can enhance the interpretations via linear, quadratic and higher-order moments of the ordered categories. To aid such interpretations, we propose a new type of graphical display-the polynomial biplot. PMID:25791164
Orthogonal bone cutting: saw design and operating characteristics.
Krause, W R
1987-08-01
The cutting process of orthopaedic bone saws was considered as orthogonal (two-dimensional) cutting for determination of the horizontal and vertical force components of single edge cutting tools with rake angles of 0 to -30 degrees. The Merchant analysis for orthogonal cutting was used to determine the resultant force and other force and work relationships. The effect of an imposed lateral vibration on the cutting tool was also investigated. The results of the tests indicated a strong interaction between the measured and derived forces with the rake angle and feed velocity. It was concluded that to reduce the cutting forces and work expenditure, a negative rake angle between 0 and -10 degrees, high feed velocity, and an imposed lateral vibration provided the greatest reduction in force and energy expenditure. PMID:3657116
External orthogonality in subsystem time-dependent density functional theory.
Chulhai, Dhabih V; Jensen, Lasse
2016-08-01
Subsystem density functional theory (subsystem DFT) is a DFT partitioning method that is exact in principle, but depends on approximations to the kinetic energy density functional (KEDF). One may avoid the use of approximate KEDFs by ensuring that the inter-subsystem molecular orbitals are orthogonal, termed external orthogonality (EO). We present a method that extends a subsystem DFT method, that includes EO, into the time-dependent DFT (TDDFT) regime. This method therefore removes the need for approximations to the kinetic energy potential and kernel, and we show that it can accurately reproduce the supermolecular TDDFT results for weakly and strongly coupled subsystems, and for systems with strongly overlapping densities (where KEDF approximations traditionally fail). PMID:26932176
Orthogonal polarization Mirau interferometer using reflective-type waveplate.
Tapilouw, Abraham Mario; Chen, Liang-Chia; Jen, Yi-Jun; Lin, Shyh-Tsong; Yeh, Sheng-Lih
2013-07-15
This work proposes an orthogonal polarization Mirau interferometry using a reflective-type waveplate to generate different polarization orientations for broadband white light interferometry. The reflective-type half-waveplate is employed as the reference arm of the Mirau interferometer to convert polarization and it generates a reference light with an orientation orthogonal to the object light. An advantage of the proposed interferometer is its ability to control the ratio of light intensity between the object and reference arms to maximize the interferometric fringe contrast. Better, more accurate calibration of standard step height has been achieved by the developed interferometer, which also can measure solder bumps that traditional Mirau interferometers usually cannot measure. PMID:23939094
Entanglement as a resource to distinguish orthogonal product states
Zhang, Zhi-Chao; Gao, Fei; Cao, Tian-Qing; Qin, Su-Juan; Wen, Qiao-Yan
2016-01-01
It is known that there are many sets of orthogonal product states which cannot be distinguished perfectly by local operations and classical communication (LOCC). However, these discussions have left the following open question: What entanglement resources are necessary and/or sufficient for this task to be possible with LOCC? In m ⊗ n, certain classes of unextendible product bases (UPB) which can be distinguished perfectly using entanglement as a resource, had been presented in 2008. In this paper, we present protocols which use entanglement more efficiently than teleportation to distinguish some classes of orthogonal product states in m ⊗ n, which are not UPB. For the open question, our results offer rather general insight into why entanglement is useful for such tasks, and present a better understanding of the relationship between entanglement and nonlocality. PMID:27458034
Orthogonally modulated molecular transport junctions for resettable electronic logic gates
NASA Astrophysics Data System (ADS)
Meng, Fanben; Hervault, Yves-Marie; Shao, Qi; Hu, Benhui; Norel, Lucie; Rigaut, Stéphane; Chen, Xiaodong
2014-01-01
Individual molecules have been demonstrated to exhibit promising applications as functional components in the fabrication of computing nanocircuits. Based on their advantage in chemical tailorability, many molecular devices with advanced electronic functions have been developed, which can be further modulated by the introduction of external stimuli. Here, orthogonally modulated molecular transport junctions are achieved via chemically fabricated nanogaps functionalized with dithienylethene units bearing organometallic ruthenium fragments. The addressable and stepwise control of molecular isomerization can be repeatedly and reversibly completed with a judicious use of the orthogonal optical and electrochemical stimuli to reach the controllable switching of conductivity between two distinct states. These photo-/electro-cooperative nanodevices can be applied as resettable electronic logic gates for Boolean computing, such as a two-input OR and a three-input AND-OR. The proof-of-concept of such logic gates demonstrates the possibility to develop multifunctional molecular devices by rational chemical design.
Orthogonal polynomial interpretation of Δ-Toda equations
NASA Astrophysics Data System (ADS)
Area, I.; Branquinho, A.; Foulquié Moreno, A.; Godoy, E.
2015-10-01
In this paper a discretization of Toda equations is analyzed. The correspondence between these Δ-Toda equations for the coefficients of the Jacobi operator and its resolvent function is established. It is shown that the spectral measure of these operators evolve in t like {(1+x)}1-t {{d}}μ (x) where {{d}}μ is a given positive Borel measure. The Lax pair for the Δ-Toda equations is derived and characterized in terms of linear functionals, where orthogonal polynomials which satisfy an Appell condition with respect to the forward difference operator Δ appear in a natural way. In order to illustrate the results of the paper we work out two examples of Δ-Toda equations related with Jacobi and Laguerre orthogonal polynomials.
A general boundary capability embedded in an orthogonal mesh
Hewett, D.W.; Yu-Jiuan Chen
1995-07-01
The authors describe how they hold onto orthogonal mesh discretization when dealing with curved boundaries. Special difference operators were constructed to approximate numerical zones split by the domain boundary; the operators are particularly simple for this rectangular mesh. The authors demonstrated that this simple numerical approach, termed Dynamic Alternating Direction Implicit, turned out to be considerably more efficient than more complex grid-adaptive algorithms that were tried previously.
Orthogonal mapping: A computer program for quantifying shape differences
Huffman, T.; Christopher, R.A.; Hazel, J.E.
1978-01-01
Quantitative differences in the shape of two or more objects can be obtained by Orthogonal Mapping provided coincidental or analogous points can be identified on each object. The least-squares difference between each set of analogous points is determined by use of a projective transformation of a set of measured points which involves the rotation, translocation, and scaling of these points relative to a set of fixed points. ?? 1978.
Star polymer synthesis viaλ-orthogonal photochemistry.
Hiltebrandt, Kai; Kaupp, Michael; Molle, Edgar; Menzel, Jan P; Blinco, James P; Barner-Kowollik, Christopher
2016-08-01
We introduce a light induced sequence enabling λ-orthogonal star polymer formation via an arms-first approach, based on an α,ω-functional polymer carrying tetrazole and o-methyl benzaldehyde moieties, which upon irradiation can readily undergo cycloaddition with a trifunctional maleimide core. Depending on the wavelength, the telechelic strand can be attached to the core at either photo-reactive end. PMID:27378494
Parsimonious extreme learning machine using recursive orthogonal least squares.
Wang, Ning; Er, Meng Joo; Han, Min
2014-10-01
Novel constructive and destructive parsimonious extreme learning machines (CP- and DP-ELM) are proposed in this paper. By virtue of the proposed ELMs, parsimonious structure and excellent generalization of multiinput-multioutput single hidden-layer feedforward networks (SLFNs) are obtained. The proposed ELMs are developed by innovative decomposition of the recursive orthogonal least squares procedure into sequential partial orthogonalization (SPO). The salient features of the proposed approaches are as follows: 1) Initial hidden nodes are randomly generated by the ELM methodology and recursively orthogonalized into an upper triangular matrix with dramatic reduction in matrix size; 2) the constructive SPO in the CP-ELM focuses on the partial matrix with the subcolumn of the selected regressor including nonzeros as the first column while the destructive SPO in the DP-ELM operates on the partial matrix including elements determined by the removed regressor; 3) termination criteria for CP- and DP-ELM are simplified by the additional residual error reduction method; and 4) the output weights of the SLFN need not be solved in the model selection procedure and is derived from the final upper triangular equation by backward substitution. Both single- and multi-output real-world regression data sets are used to verify the effectiveness and superiority of the CP- and DP-ELM in terms of parsimonious architecture and generalization accuracy. Innovative applications to nonlinear time-series modeling demonstrate superior identification results. PMID:25291736
Multilayer block copolymer meshes by orthogonal self-assembly
Tavakkoli K. G., Amir; Nicaise, Samuel M.; Gadelrab, Karim R.; Alexander-Katz, Alfredo; Ross, Caroline A.; Berggren, Karl K.
2016-01-01
Continued scaling-down of lithographic-pattern feature sizes has brought templated self-assembly of block copolymers (BCPs) into the forefront of nanofabrication research. Technologies now exist that facilitate significant control over otherwise unorganized assembly of BCP microdomains to form both long-range and locally complex monolayer patterns. In contrast, the extension of this control into multilayers or 3D structures of BCP microdomains remains limited, despite the possible technological applications in next-generation devices. Here, we develop and analyse an orthogonal self-assembly method in which multiple layers of distinct-molecular-weight BCPs naturally produce nanomesh structures of cylindrical microdomains without requiring layer-by-layer alignment or high-resolution lithographic templating. The mechanisms for orthogonal self-assembly are investigated with both experiment and simulation, and we determine that the control over height and chemical preference of templates are critical process parameters. The method is employed to produce nanomeshes with the shapes of circles and Y-intersections, and is extended to produce three layers of orthogonally oriented cylinders. PMID:26796218
Modular and Orthogonal Synthesis of Hybrid Polymers and Networks
Liu, Shuang; Dicker, Kevin T.; Jia, Xinqiao
2015-01-01
Biomaterials scientists strive to develop polymeric materials with distinct chemical make-up, complex molecular architectures, robust mechanical properties and defined biological functions by drawing inspirations from biological systems. Salient features of biological designs include (1) repetitive presentation of basic motifs; and (2) efficient integration of diverse building blocks. Thus, an appealing approach to biomaterials synthesis is to combine synthetic and natural building blocks in a modular fashion employing novel chemical methods. Over the past decade, orthogonal chemistries have become powerful enabling tools for the modular synthesis of advanced biomaterials. These reactions require building blocks with complementary functionalities, occur under mild conditions in the presence of biological molecules and living cells and proceed with high yield and exceptional selectivity. These chemistries have facilitated the construction of complex polymers and networks in a step-growth fashion, allowing facile modulation of materials properties by simple variations of the building blocks. In this review, we first summarize features of several types of orthogonal chemistries. We then discuss recent progress in the synthesis of step growth linear polymers, dendrimers and networks that find application in drug delivery, 3D cell culture and tissue engineering. Overall, orthogonal reactions and modulular synthesis have not only minimized the steps needed for the desired chemical transformations but also maximized the diversity and functionality of the final products. The modular nature of the design, combined with the potential synergistic effect of the hybrid system, will likely result in novel hydrogel matrices with robust structures and defined functions. PMID:25572255
Animation of orthogonal texture patterns for vector field visualization.
Bachthaler, Sven; Weiskopf, Daniel
2008-01-01
This paper introduces orthogonal vector field visualization on 2D manifolds: a representation by lines that are perpendicular to the input vector field. Line patterns are generated by line integral convolution (LIC). This visualization is combined with animation based on motion along the vector field. This decoupling of the line direction from the direction of animation allows us to choose the spatial frequencies along the direction of motion independently from the length scales along the LIC line patterns. Vision research indicates that local motion detectors are tuned to certain spatial frequencies of textures, and the above decoupling enables us to generate spatial frequencies optimized for motion perception. Furthermore, we introduce a combined visualization that employs orthogonal LIC patterns together with conventional, tangential streamline LIC patterns in order to benefit from the advantages of these two visualization approaches. In addition, a filtering process is described to achieve a consistent and temporally coherent animation of orthogonal vector field visualization. Different filter kernels and filter methods are compared and discussed in terms of visualization quality and speed. We present respective visualization algorithms for 2D planar vector fields and tangential vector fields on curved surfaces, and demonstrate that those algorithms lend themselves to efficient and interactive GPU implementations. PMID:18467751
Orthogonality breaking through few-mode optical fiber.
Parnet, Francois; Fade, Julien; Alouini, Mehdi
2016-04-01
Polarization sensing and imaging through optical fibers is a technological challenge motivated by promising applications for in vivo, in situ polarimetric endoscopy for biomedical diagnosis. Among the recent approaches proposed to solve this issue, the depolarization/dichroism sensing by polarization orthogonality breaking (DSOB) technique was shown to perform remotely through single-mode optical fibers for depolarization/diattenuation measurements. In this article, we investigate the applicability of such a technique in slightly multimode waveguides. Through theoretical modeling and numerical simulations, we evidence the conditions required for the polarization orthogonality to be preserved after propagation in a few-mode fiber, notably in terms of detection geometry of the spatial modes. Original experiments realized in few-mode fibers both in transmission and reflection configurations are also reported and validate the theoretical predictions. These results allow us to analyze the influence of the experimental parameters, such as detection geometry, sample tilt, or fiber length, on orthogonality preservation and on the measurement dynamics of the DSOB technique in slightly multimode waveguides. PMID:27139651
Multilayer block copolymer meshes by orthogonal self-assembly
NASA Astrophysics Data System (ADS)
Tavakkoli K. G., Amir; Nicaise, Samuel M.; Gadelrab, Karim R.; Alexander-Katz, Alfredo; Ross, Caroline A.; Berggren, Karl K.
2016-01-01
Continued scaling-down of lithographic-pattern feature sizes has brought templated self-assembly of block copolymers (BCPs) into the forefront of nanofabrication research. Technologies now exist that facilitate significant control over otherwise unorganized assembly of BCP microdomains to form both long-range and locally complex monolayer patterns. In contrast, the extension of this control into multilayers or 3D structures of BCP microdomains remains limited, despite the possible technological applications in next-generation devices. Here, we develop and analyse an orthogonal self-assembly method in which multiple layers of distinct-molecular-weight BCPs naturally produce nanomesh structures of cylindrical microdomains without requiring layer-by-layer alignment or high-resolution lithographic templating. The mechanisms for orthogonal self-assembly are investigated with both experiment and simulation, and we determine that the control over height and chemical preference of templates are critical process parameters. The method is employed to produce nanomeshes with the shapes of circles and Y-intersections, and is extended to produce three layers of orthogonally oriented cylinders.
Orthogonal reference pattern multiplexing for collinear holographic data storage.
Cao, Liangcai; Liu, Jinqiu; Li, Jianhua; He, Qingsheng; Jin, Guofan
2014-01-01
An orthogonal reference pattern multiplexing (ORPM) method for collinear holographic data storage (CHDS) is investigated to increase the data storage density and realize parallel optical image superimposition. Holograms are multiplexed in the same volume of the recording medium with multiple orthogonal reference patterns (RPs). The physical principle of this method is analyzed based on scalar diffraction theory. The orthogonal condition of the RPs is derived in order to suppress the interpage cross talk. The parameters of the radial-line RP have significant influence on the signal-to-noise ratio (SNR) of the reconstructed data page. They are optimized to reduce the intrapage cross talk. With a random binary phase mask (RBPM) located closely before the spatial light modulator, SNR of the reconstructed data page is seven times the SNR without the RBPM. Three data pages are multiplexed in the same volume of the medium using the ORPM method. The reconstructed data pages for the CHDS system show the effectiveness of the RBPM in suppressing the intrapage and interpage cross talk. PMID:24513981
Parallel and orthogonal stimulus in ultradiluted neural networks
NASA Astrophysics Data System (ADS)
Sobral, G. A., Jr.; Vieira, V. M.; Lyra, M. L.; da Silva, C. R.
2006-10-01
Extending a model due to Derrida, Gardner, and Zippelius, we have studied the recognition ability of an extreme and asymmetrically diluted version of the Hopfield model for associative memory by including the effect of a stimulus in the dynamics of the system. We obtain exact results for the dynamic evolution of the average network superposition. The stimulus field was considered as proportional to the overlapping of the state of the system with a particular stimulated pattern. Two situations were analyzed, namely, the external stimulus acting on the initialization pattern (parallel stimulus) and the external stimulus acting on a pattern orthogonal to the initialization one (orthogonal stimulus). In both cases, we obtained the complete phase diagram in the parameter space composed of the stimulus field, thermal noise, and network capacity. Our results show that the system improves its recognition ability for parallel stimulus. For orthogonal stimulus two recognition phases emerge with the system locking at the initialization or stimulated pattern. We confront our analytical results with numerical simulations for the noiseless case T=0 .
Limited-memory adaptive snapshot selection for proper orthogonal decomposition
Oxberry, Geoffrey M.; Kostova-Vassilevska, Tanya; Arrighi, Bill; Chand, Kyle
2015-04-02
Reduced order models are useful for accelerating simulations in many-query contexts, such as optimization, uncertainty quantification, and sensitivity analysis. However, offline training of reduced order models can have prohibitively expensive memory and floating-point operation costs in high-performance computing applications, where memory per core is limited. To overcome this limitation for proper orthogonal decomposition, we propose a novel adaptive selection method for snapshots in time that limits offline training costs by selecting snapshots according an error control mechanism similar to that found in adaptive time-stepping ordinary differential equation solvers. The error estimator used in this work is related to theory bounding the approximation error in time of proper orthogonal decomposition-based reduced order models, and memory usage is minimized by computing the singular value decomposition using a single-pass incremental algorithm. Results for a viscous Burgers’ test problem demonstrate convergence in the limit as the algorithm error tolerances go to zero; in this limit, the full order model is recovered to within discretization error. The resulting method can be used on supercomputers to generate proper orthogonal decomposition-based reduced order models, or as a subroutine within hyperreduction algorithms that require taking snapshots in time, or within greedy algorithms for sampling parameter space.
Minimal parameter solution of the orthogonal matrix differential equation
NASA Technical Reports Server (NTRS)
Baritzhack, Itzhack Y.; Markley, F. Landis
1988-01-01
As demonstrated in this work, all orthogonal matrices solve a first order differential equation. The straightforward solution of this equation requires n sup 2 integrations to obtain the element of the nth order matrix. There are, however, only n(n-1)/2 independent parameters which determine an orthogonal matrix. The questions of choosing them, finding their differential equation and expressing the orthogonal matrix in terms of these parameters are considered. Several possibilities which are based on attitude determination in three dimensions are examined. It is shown that not all 3-D methods have useful extensions to higher dimensions. It is also shown why the rate of change of the matrix elements, which are the elements of the angular rate vector in 3-D, are the elements of a tensor of the second rank (dyadic) in spaces other than three dimensional. It is proven that the 3-D Gibbs vector (or Cayley Parameters) are extendable to other dimensions. An algorithm is developed employing the resulting parameters, which are termed Extended Rodrigues Parameters, and numerical results are presented of the application of the algorithm to a fourth order matrix.
Minimal parameter solution of the orthogonal matrix differential equation
NASA Technical Reports Server (NTRS)
Bar-Itzhack, Itzhack Y.; Markley, F. Landis
1990-01-01
As demonstrated in this work, all orthogonal matrices solve a first order differential equation. The straightforward solution of this equation requires n sup 2 integrations to obtain the element of the nth order matrix. There are, however, only n(n-1)/2 independent parameters which determine an orthogonal matrix. The questions of choosing them, finding their differential equation and expressing the orthogonal matrix in terms of these parameters are considered. Several possibilities which are based on attitude determination in three dimensions are examined. It is shown that not all 3-D methods have useful extensions to higher dimensions. It is also shown why the rate of change of the matrix elements, which are the elements of the angular rate vector in 3-D, are the elements of a tensor of the second rank (dyadic) in spaces other than three dimensional. It is proven that the 3-D Gibbs vector (or Cayley Parameters) are extendable to other dimensions. An algorithm is developed emplying the resulting parameters, which are termed Extended Rodrigues Parameters, and numerical results are presented of the application of the algorithm to a fourth order matrix.
Quantitative determination of Piroxicam by TLC-MALDI TOF MS.
Crecelius, Anna; Clench, Malcolm R; Richards, Don S; Parr, Vic
2004-04-01
A quantitative thin-layer chromatography (TLC)-matrix-assisted laser desorption (MALDI) TOF mass spectrometry (MS) method for the determination of Piroxicam has been developed. Following preliminary experiments three different approaches to the incorporation of the internal standard (Tenoxicam) into the TLC plates were investigated. These were: (a) adding the internal standard to the mobile phase and pre-developing the plate, (b) coating the plate with internal standard by electrospraying prior to matrix application and finally, (c) mixing the internal standard into the matrix solution and electrospraying both. The most successful method was that where the internal standard was pre-developed over the plate. For this method linearity was observed over the range between 400 and 800ng of Piroxicam. The precision was found to be in the range of 1-9% R.S.D. from the average detected value (n = 5), dependent on the amount of analyte on the TLC plate. The proposed method was accurate with +/-2% deviation from the known amount of Piroxicam in the sample spot. PMID:15030877
Imaging results and TOF studies with axial PET detectors
NASA Astrophysics Data System (ADS)
Joram, Christian
2013-12-01
We have developed a fully operational PET demonstrator setup which allows true 3D reconstruction of the 511 keV photons and therefore leads to practically parallax free images. The AX-PET concept is based on thin 100 mm long scintillation crystals (LYSO), axially oriented and arranged in layers around the field of view. Layers of wavelength shifting plastic strips mounted in between the crystal layers give the axial coordinate. Both crystals and WLS strips are individually read out by G-APD (SiPM) photodetectors. The fully scalable concept overcomes the dilemma of sensitivity versus spatial resolution which is inherent to classical PET designs. A demonstrator set-up based on two axial modules was exhaustively characterized using point-like sources, phantoms filled with radiotracer and finally rats and a mouse. The results entirely meet the performance expectations (<2 mm FWHM in all three coordinates over the complete field of view) and also demonstrated the ability to include Compton interactions (inter-crystal scatter) in the reconstruction without noticeable performance loss. Our recent studies focus on a TOF extension of the AX-PET concept making use of the novel digital SiPM detectors by Philips. After reproducing comparable energy and spatial resolution on a small digital AX-PET set-up with 100 mm long crystals, we demonstrated a coincidence resolving time of about 210 ps FWHM.
Mediavilla-Gradolph, María Concepción; De Toro-Peinado, Inmaculada; Bermúdez-Ruiz, María Pilar; García-Martínez, María de los Ángeles; Ortega-Torres, María; Montiel Quezel-Guerraz, Natalia; Palop-Borrás, Begoña
2015-01-01
The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species. PMID:26106617
Toward an In Situ Organic and Atomic Microprobe with Laser TOF-MS
NASA Technical Reports Server (NTRS)
Brinckerhoff, W. B.; Cornish, T. J.; McEntire, R. W.; Cheng, A. F.; Benson, R. C.
2000-01-01
We present details of a new miniature laser time-of-flight mass spectrometer (TOF-MS) with improved resolution and sensitivity, for in situ analysis of elemental, isotopic, and organic/molecular composition.
Schiller, J; Arnhold, J; Benard, S; Reichl, S; Arnold, K
1999-05-31
Matrix-assisted laser desorption ionization and time-of-flight mass spectrometry (MALDI-TOF MS) has been used to investigate degradation products of two selected polysaccharides of cartilage (chondroitin sulfate and hyaluronic acid). Testicular hyaluronate lyase and chondroitin ABC lyase were used for enzymic digestion of both polysaccharides as well as of cartilage specimens. Polysaccharide solutions and cartilage supernatants were assayed by positive and negative MALDI-TOF MS. Especially chondroitin ABC lyase produced high amounts of digestion products (unsaturated di- and tetrasaccharides) from polysaccharides as well as from cartilage, clearly monitored by MALDI-TOF MS. It is concluded that MALDI-TOF MS provides a precise and fast tool for the determination of oligosaccharides since no previous derivatization is required. PMID:10576924
Mo, Jingxin; Eggers, Paul K; Raston, Colin L; Lim, Lee Yong
2014-04-01
Carboplatin and paclitaxel co-loaded nanovesicles (CPT-PTX-CLV), a novel intravenous formulation void of cremophor EL, may have significant advantages over conventional carboplatin and paclitaxel formulations with respect to tumor targeting, sustained drug release, reduced toxicity, and synergistic efficacy profiles. The aim of this study was to develop and validate a rapid, specific, sensitive, and reliable liquid chromatography-time of flight-mass spectrometry (LC/TOF MS)-based bioanalytical method for the simultaneous quantification of CPT and PTX in a fetal bovine serum (FBS) vehicle containing the dispersed nanovesicles. The analytes were extracted from FBS by simple protein precipitation, with subsequent separation of CPT and PTX on a Waters HPLC SunFire C18 column at a flow rate of 0.25 ml/min using gradient elution mode. The total analytical time was only 12 min. Detection and quantitation was performed by electrospray ionization (ESI) in the positive ionization mode with selective ion monitoring (SIM) at m/z 310.0152 for CPT and 876.3224 for PTX. The calibration curves were linear over the concentration range of 10-4,000 ng/ml for CPT and 5-2,000 ng/ml for PTX (r (2) > 0.99), with the respective lower limit of quantification (LLOQ) at 10 and 5 ng/ml. The intra- and inter-day precision and accuracy of analysis of the quality control samples at low, medium, and high concentration levels were ≤13.6 % relative standard deviation (RSD) and ≤14.6 % relative errors (RE). The rapid, sensitive, and reproducible LC/TOF MS method may be used to support preclinical and clinical pharmacological studies of the CPT-PTX-CLV administered by injection in animal and human cancer models. PMID:24573580
Ant colony optimization for biomarker identification from MALDI-TOF mass spectra.
Ressom, Habtom W; Varghese, Rency S; Orvisky, Eduard; Drake, Steven K; Hortin, Glen L; Abdel-Hamid, Mohamed; Loffredo, Christopher A; Goldman, Radoslav
2006-01-01
We present a novel method that combines ant colony optimization with support vector machines (ACO-SVM) to select candidate biomarkers from MALDI-TOF serum profiles of hepatocellular carcinoma (HCC) patients and matched controls. The method identified relevant mass points that achieve high sensitivity and specificity in distinguishing HCC patients from healthy individuals. The results indicate that the MALDI-TOF technology could provide the means to discover novel biomarkers for HCC. PMID:17946638
Cluster TOF-SIMS imaging as a tool for micrometric histology of lipids in tissue.
Bich, Claudia; Touboul, David; Brunelle, Alain
2014-01-01
Recent developments in instrumentation, ion beams or analyzers, for cluster time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging are described here. The methods which are employed to increase the sensitivity or to perform three-dimensional analyses in the organic materials are also illustrated. This review shows the improvements made for lipid imaging by cluster TOF-SIMS in various types of material and applications, and gives reasons for the expansion of its utilization. PMID:24265115
NASA Astrophysics Data System (ADS)
Futatani, Shimpei; Bos, Wouter J. T.; del-Castillo-Negrete, Diego; Schneider, Kai; Benkadda, Sadruddin; Farge, Marie
2011-03-01
We assess two techniques for extracting coherent vortices out of turbulent flows: the wavelet based Coherent Vorticity Extraction (CVE) and the Proper Orthogonal Decomposition (POD). The former decomposes the flow field into an orthogonal wavelet representation and subsequent thresholding of the coefficients allows one to split the flow into organized coherent vortices with non-Gaussian statistics and an incoherent random part which is structureless. POD is based on the singular value decomposition and decomposes the flow into basis functions which are optimal with respect to the retained energy for the ensemble average. Both techniques are applied to direct numerical simulation data of two-dimensional drift-wave turbulence governed by Hasegawa-Wakatani equation, considering two limit cases: the quasi-hydrodynamic and the quasi-adiabatic regimes. The results are compared in terms of compression rate, retained energy, retained enstrophy and retained radial flux, together with the enstrophy spectrum and higher order statistics.
Quantitative analysis of surface contaminants on silicon wafers by means of TOF-SIMS
NASA Astrophysics Data System (ADS)
Rostam-Khani, P.; Philipsen, J.; Jansen, E.; Eberhard, H.; Vullings, P.
2006-07-01
Measurement of surface metal contamination on silicon wafers is essential for yield enhancement in IC manufacturing. Vapour phase decomposition coupled with either inductively coupled plasma mass spectrometry (VPD-ICP-MS), or total reflection X-ray fluorescence (VPD-TXRF), TXRF and, more recently, TOF-SIMS are used to monitor surface metal contamination. These techniques complement each other in their respective strengths and weaknesses. For reliable and accurate quantification, so-called relative sensitivity factors (RSF) are required for TOF-SIMS analysis. For quantification purposes in VPD, the collection efficiency (CE) is important to ensure complete collection of contamination. A standard procedure has been developed that combines the determination of these RSFs as well as the collection efficiency using all the analytical techniques mentioned above. Therefore, sample wafers were intentionally contaminated and analysed (by TOF-SIMS) directly after preparation. After VPD-ICP-MS, several scanned surfaces were analysed again by TOF-SIMS. Comparing the intensities of the specific metals before and after the VPD-DC procedure on the scanned surface allows the determination of so-called removing efficiency (RE). In general, very good agreement was obtained comparing the analytical techniques after updating the RSFs for TOF-SIMS. Progress has been achieved concerning the CE evaluation as well as determining the RSFs for 16 elements more precisely for TOF-SIMS.
Charge transport and injection in amorphous organic electronic materials
NASA Astrophysics Data System (ADS)
Tse, Shing Chi
This thesis presents how we use various measuring techniques to study the charge transport and injection in organic electronic materials. Understanding charge transport and injection properties in organic solids is of vital importance for improving performance characteristics of organic electronic devices, including organic-light-emitting diodes (OLEDs), photovoltaic cells (OPVs), and field effect transistors (OFETs). The charge transport properties of amorphous organic materials, commonly used in organic electronic devices, are investigated by the means of carrier mobility measurements. Transient electroluminescence (EL) technique was used to evaluate the electron mobility of an electron transporting material--- tris(8-hydroxyquinoline) aluminum (Alq3). The results are in excellent agreement with independent time-of-flight (TOF) measurements. Then, the effect of dopants on electron transport was also examined. TOF technique was also used to examine the effects of tertiary-butyl (t-Bu) substitutions on anthracene derivatives (ADN). All ADN compounds were found to be ambipolar. As the degree of t-Bu substitution increases, the carrier mobilities decrease progressively. The reduction of carrier mobilities with increasing t-butylation can be attributed to a decrease in the charge-transfer integral or the wavefunction overlap. In addition, from TOF measurements, two naphthylamine-based hole transporters, namely, N,N'-diphenyl-N,N'-bis(1-naphthyl)(1,1'-biphenyl)-4,4'diamine (NPB) and 4,4',4"-tris(n-(2-naphthyl)-n-phenyl-amino)-triphenylamine (2TNATA) were found to possess electron-transporting (ET) abilities. An organic light-emitting diode that employed NPB as the ET material was demonstrated. The electron conducting mechanism of NPB and 2TNATA in relation to the hopping model will be discussed. Furthermore, the ET property of NPB applied in OLEDs will also be examined. Besides transient EL and TOF techniques, we also use dark-injection space-charge-limited current
Husáková, Lenka; Urbanová, Iva; Šrámková, Jitka; Konečná, Michaela; Bohuslavová, Jana
2013-03-15
In this work a simple technique employing oxalic and nitric acid to cow's milk samples prior to analysis by inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometry (ICP-oa-TOF-MS) was introduced. After the precipitation of calcium and proteins via oxalic and nitric acid, respectively, the resulting liquid phase was aspirated with a concentric glass nebulizer for ICP-TOF-MS determination of trace elements. Precipitation of proteins is essential for better separation of solid and liquid phase of modified samples. Separation of calcium as a precipitated non-soluble oxalate enables the elimination of spectral interferences originating from different calcium containing species like (40)Ca(35)Cl(+), (40)Ca(37)Cl(+), (43)Ca(16)O(+), (40)Ca(18)O(+), (44)Ca(16)O(+), (43)Ca(16)O(1)H(+) onto the determination of As, Se, Co and Ni whose assay is more difficult when using conventional quadrupole instruments. High detection capability is further an advantage as the approach enables the analysis without dilution. The methodology may serve, in addition, for a fast and sensitive determination of some other elements. After that, direct, reliable and simultaneous determination of 16 elements (Li, Be, B, V, Cr, Mn, Ni, Co, Ga, As, Se, Mo, Sn, Sb, Cs, Tl) at trace and ultra-trace levels in milk can be performed under optimum instrumental conditions and by using Rh as an internal standard. Accuracy and precision was assessed by measuring NCS ZC73015 milk powder control standard, yielding results in agreement with certified values and RSD <10%. The accuracy was also checked by comparison of the results of the proposed method with those found by a method based on a microwave-assisted digestion of real samples. PMID:23598096
Ji, Jian; Zhu, Pei; Pi, Fuwei; Sun, Chao; Jiang, Hui; Sun, Jiadi; Wang, Xiumei; Zhang, Yinzhi; Sun, Xiulan
2016-09-15
The actual health risk from exposure to combined mycotoxins is unknown, and few studies have focused on changes to cellular biological systems (e.g., metabolomics) caused by combined mycotoxic effects. To evaluate the combined mycotoxic effects of deoxynivalenol (DON) and zearalenone (ZEN) on the level of cellular biological systems, gas chromatographic, time-of-flight mass spectroscopy (GC-TOF/MS) of the complete murine macrophage ANA-1 cell metabolome was implemented in this study. Using optimized chromatography and mass spectrometry parameters, the metabolites detected by GC-TOF/MS were identified and processed using multivariate statistical analysis, including principal component analysis (PCA) and orthogonal projection on latent-structures discriminant analysis (OPLS-DA). The metabolite sets were screened for further pathway analysis under rules of t-test (P) value < 0.05, VIP value > 1, and similarity value > 500. The mainly interfered metabolism pathways were categorized into two dominant types: amino acid metabolism and glycometabolism. Four metabolites, palmitic acid, 1-monopalmitin, ribose-5-phosphate and 2-deoxy-D-galactose, occur only under combined "DON + ZEN" treatment, indicating abnormal metabolism in ANA-1 cells. The metabolic state of ANA-1 cells under induction by combined "DON + ZEN" illustrates that DON may inhibit the estrogenic effects of ZEN. Thus, the combined effect of "DON + ZEN" may exacerbate toxicity in the pentose phosphate pathway, while palmitic acid metabolism is likely a new pathway effected by the combination, "DON + ZEN." PMID:27530666
Fast SiPM Readout of the PANDA TOF Detector
NASA Astrophysics Data System (ADS)
Böhm, M.; Lehmann, A.; Motz, S.; Uhlig, F.
2016-05-01
For the identification of low momentum charged particles and for event timing purposes a barrel Time-of-Flight (TOF) detector surrounding the interaction point is planned for the PANDA experiment at FAIR . Since the boundary conditions in terms of available radial space and radiation length are quite strict the favored layout is a hodoscope composed of several thousand small scintillating tiles (SciTils) read out by silicon photomultipliers (SiPMs). A time resolution of well below 100 ps is aimed for. With the originally proposed 30 × 30 × 5 mm3 SciTils read out by two single 3 × 3 mm2 SiPMs at the rims of the scintillator the targeted time resolution can be just reached, but with a considerable position dependence across the scintillator surface. In this paper we discuss other design options to further improve the time resolution and its homogeneity. It will be shown that wide scintillating rods (SciRods) with a size of, e.g., 50 × 30 × 5 mm3 or longer and read out at opposite sides by a chain of four serially connected SiPMs a time resolution down to 50 ps can be reached without problems. In addition, the position dependence of the time resolution is negligible. These SciRods were tested in the laboratory with electrons of a 90Sr source and under real experimental conditions in a particle beam at CERN. The measured time resolutions using fast BC418 or BC420 plastic scintillators wrapped in aluminum foil were consistently between 45 and 75 ps dependent on the SciRod design. This is a significant improvement compared to the original SciTil layout.
Heat welding of non-orthogonal X-junction of single-walled carbon nanotubes
NASA Astrophysics Data System (ADS)
Yang, Xueming; Han, Zhonghe; Li, Yonghua; Chen, Dongci; Zhang, Pu; To, Albert C.
2012-09-01
Though X-junctions of single-walled carbon nanotubes (SWCNTs) have been intensively studied, studies concerning non-orthogonal X-junctions are still very rare. In this paper, the heat welding of defect-free non-orthogonal X-junctions with different crossed angles are investigated by molecular dynamics simulations. The difference between the heat welding of non-orthogonal and orthogonal X-junctions is described, and the angle effect on the configuration and stability of the heat welded non-orthogonal X-junctions is discussed. Compared with the orthogonal X-junction, two crossed SWCNTs with a smaller non-orthogonal angle are easier to join by heat welding, and this may be an important reason why the large tubes are difficult to join, whereas large nanotube bundles are easier to observe in experiments.
Integrated, Dual Orthogonal Antennas for Polarimetric Ground Penetrating Radar
NASA Astrophysics Data System (ADS)
Pauli, Mario; Wiesbeck, Werner
2015-04-01
Ground penetrating radar systems are mostly equipped with single polarized antennas, for example with single linear polarization or with circular polarization. The radiated waves are partly reflected at the ground surface and very often the penetrating waves are distorted in their polarization. The distortion depends on the ground homogeneity and the orientation of the antennas relative to the ground structure. The received signals from the reflecting objects may most times only be classified according to their coverage and intensity. This makes the recognition of the objects difficult or impossible. In airborne and spaceborne Remote Sensing the systems are meanwhile mostly equipped with front ends with dual orthogonal polarized antennas for a full polarimetric operation. The received signals, registered in 2x2 scattering matrices according to co- and cross polarization, are processed for the evaluation of all features of the targets. Ground penetrating radars could also profit from the scientific results of Remote Sensing. The classification of detected objects for their structure and orientation requires more information in the reflected signal than can be measured with a single polarization [1, 2]. In this paper dual linear, orthogonal polarized antennas with a common single, frequency independent phase center, are presented [3]. The relative bandwidth of these antennas can be 1:3, up to 1:4. The antenna is designed to work in the frequency range between 3 GHz and 11 GHz, but can be easily adapted to the GPR frequency range by scaling. The size of the antenna scaled for operation in typical GPR frequencies would approximately be 20 by 20 cm2. By the implementation in a dielectric carrier it could be reduced in size if required. The major problem for ultra wide band, dual polarized antennas is the frequency independent feed network, realizing the required phase shifts. For these antennas a network, which is frequency independent over a wide range, has been
Resolution enhancement of computed radiography images using two orthogonal tilts
NASA Astrophysics Data System (ADS)
Pollmann, Steven I.; Norley, Chris J. D.; Yuan, Xunhua; Holdsworth, David W.
2012-03-01
Limitations to the spatial resolution of current digital x-ray systems are bounded by the physical characteristics of the xray detector. However, the need to image smaller structures provides motivation to develop high-resolution x-ray detector systems for use with computed radiographic, and tomographic x-ray systems. We report the implementation of a tilted detector technique (TDT) to attain near isotropic resolution enhancement by combining two orthogonal image views, acquired with existing detector hardware tilted at a fixed angle. Images were acquired using a ceiling-mounted x-ray unit (Proteus XR/a, GE Medical Systems, 50kVp, 250mAs). Images were digitized using a Fujifilm Capsula X CR system, from a 35×43cm detector cassette placed on an angulated stand, featuring a 3520×4280 image matrix with an in-plane pixel spacing of 0.1mm. Three images were acquired: two for use with our TDT; and one for comparison, with no detector tilt. Performance was determined by using two line-pair phantoms (Models 07-521 and 07-533, Nuclear Associates) placed orthogonally to each other in the field of view. Custom software corrected for perspective distortion, co-registered and combined the tilted-detector images into a single higher-resolution image. Following unwarping and co-registration, the limiting spatial resolution of an image obtained via the weighted combination of the two orthogonal views (8 lp/mm) is found to be superior to that of a single view acquired with no detector tilt (5 lp/mm). This novel technique shows significant improvement in the spatial resolution of x-ray image acquisitions, using existing x-ray components and detector hardware.
NASA Astrophysics Data System (ADS)
Fischer, W.; Hahn, H.; Mackay, W. W.; Tsoupas, N.
1997-05-01
During the RHIC sextant test in January 1997 beam was injected into a sixth of one of the rings for the first time. We describe the injection zone and its bottlenecks, the application program to steer the beam and the injection kickers. We report on the commissioning of the injection systems and on measurements of the kickers.
Magnetic particle detection in unshielded environment using orthogonal fluxgate gradiometer
NASA Astrophysics Data System (ADS)
Elrefai, Ahmed L.; Sasada, Ichiro
2015-05-01
A new detection system for magnetic particles, which can operate in an unshielded environment, is developed using a fundamental mode orthogonal fluxgate gradiometer. The proposed detection system offers the advantages of cost, size, and weight reduction as compared to contamination detection systems using superconducting quantum interference device sensor. The detection system can be used to detect metallic contamination in foods or lithium ion battery production lines. The system has been investigated numerically to optimize various design parameters of the system. Experimental setup has been developed to evaluate some of the numerically predicted results. Steel balls were successfully detected down to the diameter of 50 μm.
Transfer Function Identification Using Orthogonal Fourier Transform Modeling Functions
NASA Technical Reports Server (NTRS)
Morelli, Eugene A.
2013-01-01
A method for transfer function identification, including both model structure determination and parameter estimation, was developed and demonstrated. The approach uses orthogonal modeling functions generated from frequency domain data obtained by Fourier transformation of time series data. The method was applied to simulation data to identify continuous-time transfer function models and unsteady aerodynamic models. Model fit error, estimated model parameters, and the associated uncertainties were used to show the effectiveness of the method for identifying accurate transfer function models from noisy data.
Aspects of surface generation in orthogonal ultraprecision machining
Lucca, D.A.; Seo, Y.W.; Rhorer, R.L.
1994-10-01
The depth of the plastically deformed layer at the workpiece surface which resulted in the orthogonal ultraprecision machining of Cu over the range of uncut chip thicknesses of 0.01-10 {mu}m was investigated. Two tools with the same nominal geometry but with differing edge geometries were used to machine both Te-Cu and fine grain Cu. Tool edge geometries were characterized by atomic force microscopy, taking into account the AFM cantilever tip radius. Magnitudes of the measured depths appear to be consistent with values reported in the literature and those arrived at by simple analyses.
Basis difference method for orthogonal systems on a surface
NASA Astrophysics Data System (ADS)
Korobitsyn, V. A.
2011-07-01
The basis operator method intended for constructing systems of difference approximations to differential operators in vector and tensor analysis is extended to orthogonal systems on a surface. A class of completely conservative differential-difference schemes for continuum mechanics in Lagrangian variables is constructed. Basis operators are constructed using the finite volume equation, consistency conditions for discrete operators of the first derivative, and consistent projection operators for grid functions. A system of differential-difference continuum mechanics equations on a surface is obtained, which implies all conservation laws typical of the continuum case, including additional ones. A stability estimate is derived for discrete equations of an incompressible viscous fluid.
Orthogonal (transverse) arrangements of actin in endothelia and fibroblasts
Curtis, Adam; Aitchison, Gregor; Tsapikouni, Theodora
2006-01-01
Though actin filaments running across the cell (transverse actin) have been occasionally reported for epithelial cells in groups and for cells growing on fibres, there has been no report heretofore of transverse actin in cells grown on planar substrata. This paper describes evidence in support of this possibility derived from actin staining, polarization microscopy and force measurements. The paper introduces two new methods for detecting the orientation and activity of contractile elements in cells. The orthogonal actin is most obvious in cells grown on groove ridge structures, but can be detected in cells grown on flat surfaces. PMID:17015307
The Local Orthogonality Between Quantum States and Entanglement Decomposition
NASA Astrophysics Data System (ADS)
Kim, Sunho; Wu, Junde; Zhang, Lin; Cho, Minhyung
2016-06-01
In the paper, we show that when a quantum state can be decomposed as a convex combination of locally orthogonal mixed states, its entanglement can be decomposed into the entanglement of these mixed states without losing them. The obtained result generalizes a corresponding one proved by Horodecki (Acta Phys. Slov. 48, 141 1998). But, for the entanglement cost it requires certain conditions for holding the decomposition, and the distillable entanglement only has a week result as inequality. Finally, we presented an example to show that the conditions of our conclusions are existence.
Recurrence relations of the multi-indexed orthogonal polynomials. III
NASA Astrophysics Data System (ADS)
Odake, Satoru
2016-02-01
In Paper II, we presented conjectures of the recurrence relations with constant coefficients for the multi-indexed orthogonal polynomials of Laguerre, Jacobi, Wilson, and Askey-Wilson types. In this paper we present a proof for the Laguerre and Jacobi cases. Their bispectral properties are also discussed, which gives a method to obtain the coefficients of the recurrence relations explicitly. This paper extends to the Laguerre and Jacobi cases the bispectral techniques recently introduced by Gómez-Ullate et al. [J. Approx. Theory 204, 1 (2016); e-print arXiv:1506.03651 [math.CA
Capillary-Driven Flow in Liquid Filaments Connecting Orthogonal Channels
NASA Technical Reports Server (NTRS)
Allen, Jeffrey S.
2005-01-01
Capillary phenomena plays an important role in the management of product water in PEM fuel cells because of the length scales associated with the porous layers and the gas flow channels. The distribution of liquid water within the network of gas flow channels can be dramatically altered by capillary flow. We experimentally demonstrate the rapid movement of significant volumes of liquid via capillarity through thin liquid films which connect orthogonal channels. The microfluidic experiments discussed provide a good benchmark against which the proper modeling of capillarity by computational models may be tested. The effect of surface wettability, as expressed through the contact angle, on capillary flow will also be discussed.
Conditioning analysis of incomplete Cholesky factorizations with orthogonal dropping
Napov, Artem
2012-03-16
The analysis of preconditioners based on incomplete Cholesky factorization in which the neglected (dropped) components are orthogonal to the approximations being kept is presented. General estimate for the condition number of the preconditioned system is given which only depends on the accuracy of individual approximations. The estimate is further improved if, for instance, only the newly computed rows of the factor are modified during each approximation step. In this latter case it is further shown to be sharp. The analysis is illustrated with some existing factorizations in the context of discretized elliptic partial differential equations.
Orthogonal cutting characteristics of graphite/epoxy composite material
NASA Astrophysics Data System (ADS)
Wang, D. H.; Ramulu, M.; Wern, C. W.
Orthogonal cutting experimental study was conducted to investigate the machining characteristics of Graphite/Epoxy (Gr/Ep) composite material. Machining characteristics were evaluated in terms of cutting forces, chip formation, and surface morphology of unidirectional Gr/Ep composite material of different fiber orientations. The cutting forces were measured by a three-dimensional circular-type dynamometer. Chips were examined under Scanning Electron Microscopy (SEM) and the machined surface morphology was investigated by measuring the surface roughness and by observing SEM photographs. Cutting forces, chip formation process and the surface morphology of machined surface were found to be highly dependent on the fiber orientations with respect to the cutting direction.
Variability in GRB light curves: Introducing Orthogonal Matching Pursuit
NASA Astrophysics Data System (ADS)
Dereli, Husne; Bégué, Damien; Ryde, Felix
2016-07-01
Constraining the variability of GRBs is important as it is one of the few keys to estimate many unknown parameters, such as the emission radius, the Lorentz factor, the size of the progenitor. In this work, we introduced the Orthogonal Matching Pursuit (OMP) method to study GRB light curves and to compute the minimum time variability of GRBs. Commonly used in medical sciences, this method reconstructs a signal by choosing among predefined functional shapes. We will discuss the implementation of the code, and compare its performances with those of other dedicated methods (Haar wavelet analysis, peak finding algorithm and step wise filter correlation).
Spin Symmetry and Size Consistency of Strongly Orthogonal Geminals.
Jeszenszki, Péter; Surján, Péter R; Szabados, Ágnes
2015-07-14
An overview of geminal-based wavefunctions is given, allowing for singlet-triplet mixing within the two-electron units. Spin contamination of the total wavefunction (obtained as an antisymmetrized product) is restored by spin projection. Full variation after projection is examined for two models. One is the long known spin-projected, extended Hartree-Fock (EHF). The other is a yet unexplored function, termed spin-projected, extended antisymmetrized product of strongly orthogonal geminals (EAPSG). Studies on size consistency are presented for both models. Numerical evaluation of EHF and EAPSG is performed for small test systems (H4 and H8). PMID:26575746
Orthogonal linear group-subgroup pairs with the same invariants
NASA Astrophysics Data System (ADS)
Solomon, S.
2005-03-01
The main theorem of Galois theory states that there are no finite group-subgroup pairs with the same invariants. On the other hand, if we consider complex linear reductive groups instead of finite groups, the analogous statement is no longer true: There exist counterexample group-subgroup pairs with the same invariants. However, it's possible to classify all these counterexamples for certain types of groups. In [16], we provided the classification for connected complex irreducible groups, and, in this paper, for connected complex orthogonal groups, i.e., groups that preserve some non-degenerate quadratic form.
Universality of Mesoscopic Fluctuations for Orthogonal Polynomial Ensembles
NASA Astrophysics Data System (ADS)
Breuer, Jonathan; Duits, Maurice
2016-03-01
We prove that the fluctuations of mesoscopic linear statistics for orthogonal polynomial ensembles are universal in the sense that two measures with asymptotic recurrence coefficients have the same asymptotic mesoscopic fluctuations (under an additional assumption on the local regularity of one of the measures). The convergence rate of the recurrence coefficients determines the range of scales on which the limiting fluctuations are identical. Our main tool is an analysis of the Green's function for the associated Jacobi matrices.As a particular consequencewe obtain a central limit theorem for the modified Jacobi Unitary Ensembles on all mesoscopic scales.
Proton lifetime in orthogonal theories of family unification
NASA Astrophysics Data System (ADS)
Bagger, Jonathan; Dimopoulos, Savas; Massó, Eduard
1984-09-01
We compute the proton lifetime in recently proposed orthogonal theories of family unification. For ΛMS = 100 MeV, we find a partial lifetime τ(p-->e+π0) of 5.9×1031+/-1 yr, where the error in the exponent comes from uncertainties in the hadronic wave function. Important decay products include electrons, neutrinos and non-strange mesons. Fulbright Fellow. On leave of absence from Departament de Física Teòrica, Universitat Autónoma de Barcelona, Belaterra, Spain.
Magnetic particle detection in unshielded environment using orthogonal fluxgate gradiometer
Elrefai, Ahmed L. Sasada, Ichiro
2015-05-07
A new detection system for magnetic particles, which can operate in an unshielded environment, is developed using a fundamental mode orthogonal fluxgate gradiometer. The proposed detection system offers the advantages of cost, size, and weight reduction as compared to contamination detection systems using superconducting quantum interference device sensor. The detection system can be used to detect metallic contamination in foods or lithium ion battery production lines. The system has been investigated numerically to optimize various design parameters of the system. Experimental setup has been developed to evaluate some of the numerically predicted results. Steel balls were successfully detected down to the diameter of 50 μm.
Emami, Kaveh; Nelson, Andrew; Hack, Ethan; Zhang, Jinwei; Green, David H.; Caldwell, Gary S.; Mesbahi, Ehsan
2016-01-01
The genus Pseudoalteromonas constitutes an ecologically significant group of marine Gammaproteobacteria with potential biotechnological value as producers of bioactive compounds and of enzymes. Understanding their roles in the environment and bioprospecting for novel products depend on efficient ways of identifying environmental isolates. Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) biotyping has promise as a rapid and reliable method of identifying and distinguishing between different types of bacteria, but has had relatively limited application to marine bacteria and has not been applied systematically to Pseudoalteromonas. Therefore, we constructed a MALDI-TOF MS database of 31 known Pseudoalteromonas species, to which new isolates can be compared by MALDI-TOF biotyping. The ability of MALDI-TOF MS to distinguish between species was scrutinized by comparison with 16S rRNA gene sequencing. The patterns of similarity given by the two approaches were broadly but not completely consistent. In general, the resolution of MALDI-TOF MS was greater than that of 16S rRNA gene sequencing. The database was tested with 13 environmental Pseudoalteromonas isolates from UK waters. All of the test strains could be identified to genus level by MALDI-TOF MS biotyping, but most could not be definitely identified to species level. We conclude that several of these isolates, and possibly most, represent new species. Thus, further taxonomic investigation of Pseudoalteromonas is needed before MALDI-TOF MS biotyping can be used reliably for species identification. It is, however, a powerful tool for characterizing and distinguishing among environmental isolates and can make an important contribution to taxonomic studies. PMID:26903983
Emami, Kaveh; Nelson, Andrew; Hack, Ethan; Zhang, Jinwei; Green, David H; Caldwell, Gary S; Mesbahi, Ehsan
2016-01-01
The genus Pseudoalteromonas constitutes an ecologically significant group of marine Gammaproteobacteria with potential biotechnological value as producers of bioactive compounds and of enzymes. Understanding their roles in the environment and bioprospecting for novel products depend on efficient ways of identifying environmental isolates. Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) biotyping has promise as a rapid and reliable method of identifying and distinguishing between different types of bacteria, but has had relatively limited application to marine bacteria and has not been applied systematically to Pseudoalteromonas. Therefore, we constructed a MALDI-TOF MS database of 31 known Pseudoalteromonas species, to which new isolates can be compared by MALDI-TOF biotyping. The ability of MALDI-TOF MS to distinguish between species was scrutinized by comparison with 16S rRNA gene sequencing. The patterns of similarity given by the two approaches were broadly but not completely consistent. In general, the resolution of MALDI-TOF MS was greater than that of 16S rRNA gene sequencing. The database was tested with 13 environmental Pseudoalteromonas isolates from UK waters. All of the test strains could be identified to genus level by MALDI-TOF MS biotyping, but most could not be definitely identified to species level. We conclude that several of these isolates, and possibly most, represent new species. Thus, further taxonomic investigation of Pseudoalteromonas is needed before MALDI-TOF MS biotyping can be used reliably for species identification. It is, however, a powerful tool for characterizing and distinguishing among environmental isolates and can make an important contribution to taxonomic studies. PMID:26903983
MALDI-TOF mass spectrometry - a rapid method for the identification of dermatophyte species.
Nenoff, Pietro; Erhard, Marcel; Simon, Jan C; Muylowa, Grace K; Herrmann, Jürgen; Rataj, Waldemar; Gräser, Yvonne
2013-01-01
Altogether 285 dermatophyte isolates of 21 different species - including both Trichophyton rubrum and T. interdigitale, but also eight additional Trichophyton species, Microsporum canis and seven other Microsporum species, as well as Epidermophyton floccosum and Arthroderma spp. - were analyzed using Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and the AnagnosTec 'SARAMIS' (Spectral Archiving and Microbial Identification System) software. In addition, sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA was performed for a high number of the tested strains. Sufficient agreement was found between the results obtained with standard identification methods and those with the MALDI-TOF MS for species identification of dermatophytes. A mass spectra database was constructed which contained the species identifications of all 285 isolates. The results were confirmed for 164 of the isolates by sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA. Statistical analysis of all 285 dermatophyte strains showed that conventional identification matched the results of MALDI-TOF MS for 78.2% of the isolates tested. In the case of the 164 isolates for which the identifications were confirmed by PCR, the results of their conventional diagnosis and MALDI-TOF MS were in agreement for only 68.9 % (113 of 164 strains) of the test isolates. In contrast, there was agreement of 99.3 % or 98.8 % in the identifications obtained with PCR and MALDI-TOF MS techniques (283/285 or 162/164). The two exceptions were isolates that proved to be T. violaceum which could not be identified by the MALDI-TOF MS technique. In conclusion, the MALDI-TOF mass spectroscopy represents a fast and very specific method for species differentiation of dermatophytes grown in culture. PMID:22574631
Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases.
Sagredo, Sandra; Pirzer, Tobias; Aghebat Rafat, Ali; Goetzfried, Marisa A; Moncalian, Gabriel; Simmel, Friedrich C; de la Cruz, Fernando
2016-03-18
DNA-binding proteins are promising reagents for the sequence-specific modification of DNA-based nanostructures. Here, we investigate the utility of a series of relaxase proteins-TrwC, TraI, and MobA-for nanofunctionalization. Relaxases are involved in the conjugative transfer of plasmids between bacteria, and bind to their DNA target sites via a covalent phosphotyrosine linkage. We study the binding of the relaxases to two standard DNA origami structures-rodlike six-helix bundles and flat rectangular origami sheets. We find highly orthogonal binding of the proteins with binding yields of 40-50 % per binding site, which is comparable to other functionalization methods. The yields differ for the two origami structures and also depend on the position of the binding sites. Due to their specificity for a single-stranded DNA target, their orthogonality, and their binding properties, relaxases are a uniquely useful addition to the toolbox available for the modification of DNA nanostructures with proteins. PMID:26915475
Xylonucleic acid: synthesis, structure, and orthogonal pairing properties
Maiti, Mohitosh; Maiti, Munmun; Knies, Christine; Dumbre, Shrinivas; Lescrinier, Eveline; Rosemeyer, Helmut; Ceulemans, Arnout; Herdewijn, Piet
2015-01-01
There is a common interest for studying xeno-nucleic acid systems in the fields of synthetic biology and the origin of life, in particular, those with an engineered backbone and possessing novel properties. Along this line, we have investigated xylonucleic acid (XyloNA) containing a potentially prebiotic xylose sugar (a 3′-epimer of ribose) in its backbone. Herein, we report for the first time the synthesis of four XyloNA nucleotide building blocks and the assembly of XyloNA oligonucleotides containing all the natural nucleobases. A detailed investigation of pairing and structural properties of XyloNAs in comparison to DNA/RNA has been performed by thermal UV-melting, CD, and solution state NMR spectroscopic studies. XyloNA has been shown to be an orthogonal self-pairing system which adopts a slightly right-handed extended helical geometry. Our study on one hand, provides understanding for superior structure-function (-pairing) properties of DNA/RNA over XyloNA for selection as an informational polymer in the prebiotic context, while on the other hand, finds potential of XyloNA as an orthogonal genetic system for application in synthetic biology. PMID:26175047
Geometric Attitude Controls And Estimations On The Special Orthogonal Group
NASA Astrophysics Data System (ADS)
Wu, Tse-Huai
This dissertation is concerned with spacecraft attitude control and estimation problems from the point of view of geometric mechanics. The controllers and observers are built on the special orthogonal group without any parameterizations, where the attitude dynamics is treated in a global and unique manner. The dissertation is composed of three parts. A leader-follower attitude formation control scheme is reported such that the leader spacecraft control its absolute attitude with respect to the inertial reference frame and the follower spacecraft control relative attitude with respect to other spacecraft in the formation. The unique feature is that both the absolute attitude and the relative attitude control systems are developed directly in terms of the line-of-sight observations, where attitude determination and estimation processes are not required. Second, an angular velocity observer is developed such that the estimated angular velocity is guaranteed to converge to the true angular velocity asymptotically from almost all initial estimates. Then, the presented observer is integrated with a proportional-derivative attitude tracking controller to show a separation type property for attitude tracking in the absence of angular velocity measurements. A hybrid observer for the attitude dynamics of a rigid body is proposed to guarantee global asymptotic stability. By designing a set of attitude error functions, attitude estimates are expelled from undesired equilibria to achieve global asymptotic stability. To guarantee that the estimated attitudes evolve on the special orthogonal group, a numerical algorithm based on the Lie group method is presented.
Orthogonal Cherenkov sound in spin-orbit coupled systems
NASA Astrophysics Data System (ADS)
Smirnov, Sergey
2015-06-01
Conventionally the Cherenkov sound is governed by orbital degrees of freedom and is excited by supersonic particles. Additionally, it usually has a forward nature with a conic geometry known as the Cherenkov cone whose axis is oriented along the supersonic particle motion. Here we predict Cherenkov sound of a unique nature entirely resulting from the electronic spin degree of freedom and demonstrate a fundamentally distinct Cherenkov effect originating from essentially subsonic electrons in two-dimensional gases with both Bychkov-Rashba and Dresselhaus spin-orbit interactions. Specifically, we show that the axis of the conventional forward Cherenkov cone gets a nontrivial quarter-turn and at the same time the sound distribution strongly localizes around this rotated axis being now orthogonal to the subsonic particle motion. Apart from its fundamentally appealing nature, the orthogonal Cherenkov sound could have applications in planar semiconductor technology combining spin and acoustic phenomena to develop, e.g., acoustic amplifiers or sound sources with a flexible spin dependent orientation of the sound propagation.
Impact Damage of 3D Orthogonal Woven Composite Circular Plates
NASA Astrophysics Data System (ADS)
Ji, Changgan; Sun, Baozhong; Qiu, Yiping; Gu, Bohong
2007-11-01
The damages of 3D orthogonal woven composite circular plate under quasi-static indentation and transverse impact were tested with Materials Test System (MTS) and modified split Hopkinson bar (SHPB) apparatus. The load vs. displacement curves during quasi-static penetration and impact were obtained to study the energy absorption of the composite plate. The fluctuation of the impact stress waves has been unveiled. Differences of the load-displacement curves between the quasi-static and impact loading are discussed. This work also aims at establishing a unit-cell model to analyze the damage of composites. A user material subroutine which named VUMAT for characterizing the constitutive relationship of the 3-D orthogonal woven composite and the damage evolution is incorporated with a finite element code ABAQUS/Explicit to simulate the impact damage process of the composite plates. From the comparison of the load-displacement curves and energy absorption curves of the composite plate between experimental and FEM simulation, it is shown that the unit-cell model of the 3D woven composite and the VUMAT combined with the ABAQUS/Explicit can calculate the impact responses of the circular plate precisely. Furthermore, the model can also be extended to simulate the impact behavior of the 3D woven composite structures.
Cerenkov luminescence tomography based on preconditioning orthogonal matching pursuit
NASA Astrophysics Data System (ADS)
Liu, Haixiao; Hu, Zhenhua; Wang, Kun; Tian, Jie; Yang, Xin
2015-03-01
Cerenkov luminescence imaging (CLI) is a novel optical imaging method and has been proved to be a potential substitute of the traditional radionuclide imaging such as positron emission tomography (PET) and single-photon emission computed tomography (SPECT). This imaging method inherits the high sensitivity of nuclear medicine and low cost of optical molecular imaging. To obtain the depth information of the radioactive isotope, Cerenkov luminescence tomography (CLT) is established and the 3D distribution of the isotope is reconstructed. However, because of the strong absorption and scatter, the reconstruction of the CLT sources is always converted to an ill-posed linear system which is hard to be solved. In this work, the sparse nature of the light source was taken into account and the preconditioning orthogonal matching pursuit (POMP) method was established to effectively reduce the ill-posedness and obtain better reconstruction accuracy. To prove the accuracy and speed of this algorithm, a heterogeneous numerical phantom experiment and an in vivo mouse experiment were conducted. Both the simulation result and the mouse experiment showed that our reconstruction method can provide more accurate reconstruction result compared with the traditional Tikhonov regularization method and the ordinary orthogonal matching pursuit (OMP) method. Our reconstruction method will provide technical support for the biological application for Cerenkov luminescence.
Tomographic Approach in Three-Orthogonal-Basis Quantum Key Distribution
NASA Astrophysics Data System (ADS)
Liang, Wen-Ye; Wen, Hao; Yin, Zhen-Qiang; Chen, Hua; Li, Hong-Wei; Chen, Wei; Han, Zheng-Fu
2015-09-01
At present, there is an increasing awareness of some three-orthogonal-basis quantum key distribution protocols, such as, the reference-frame-independent (RFI) protocol and the six-state protocol. For secure key rate estimations of these protocols, there are two methods: one is the conventional approach, and another is the tomographic approach. However, a comparison between these two methods has not been given yet. In this work, with the general model of rotation channel, we estimate the key rate using conventional and tomographic methods respectively. Results show that conventional estimation approach in RFI protocol is equivalent to tomographic approach only in the case of that one of three orthogonal bases is always aligned. In other cases, tomographic approach performs much better than the respective conventional approaches of the RFI protocol and the six-state protocol. Furthermore, based on the experimental data, we illustrate the deep connections between tomography and conventional RFI approach representations. Supported by the National Basic Research Program of China under Grant Nos. 2011CBA00200 and 2011CB921200 and the National Natural Science Foundation of China under Grant Nos. 60921091, 61475148, and 61201239 and Zhejiang Natural Science Foundation under Grant No. LQ13F050005
Orthogonalizing EM: A design-based least squares algorithm
Xiong, Shifeng; Dai, Bin; Huling, Jared; Qian, Peter Z. G.
2016-01-01
We introduce an efficient iterative algorithm, intended for various least squares problems, based on a design of experiments perspective. The algorithm, called orthogonalizing EM (OEM), works for ordinary least squares and can be easily extended to penalized least squares. The main idea of the procedure is to orthogonalize a design matrix by adding new rows and then solve the original problem by embedding the augmented design in a missing data framework. We establish several attractive theoretical properties concerning OEM. For the ordinary least squares with a singular regression matrix, an OEM sequence converges to the Moore-Penrose generalized inverse-based least squares estimator. For ordinary and penalized least squares with various penalties, it converges to a point having grouping coherence for fully aliased regression matrices. Convergence and the convergence rate of the algorithm are examined. Finally, we demonstrate that OEM is highly efficient for large-scale least squares and penalized least squares problems, and is considerably faster than competing methods when n is much larger than p. Supplementary materials for this article are available online. PMID:27499558
Improved analytical model for residual stress prediction in orthogonal cutting
NASA Astrophysics Data System (ADS)
Qi, Zhaoxu; Li, Bin; Xiong, Liangshan
2014-09-01
The analytical model of residual stress in orthogonal cutting proposed by Jiann is an important tool for residual stress prediction in orthogonal cutting. In application of the model, a problem of low precision of the surface residual stress prediction is found. By theoretical analysis, several shortages of Jiann's model are picked out, including: inappropriate boundary conditions, unreasonable calculation method of thermal stress, ignorance of stress constraint and cyclic loading algorithm. These shortages may directly lead to the low precision of the surface residual stress prediction. To eliminate these shortages and make the prediction more accurate, an improved model is proposed. In this model, a new contact boundary condition between tool and workpiece is used to make it in accord with the real cutting process; an improved calculation method of thermal stress is adopted; a stress constraint is added according to the volumeconstancy of plastic deformation; and the accumulative effect of the stresses during cyclic loading is considered. At last, an experiment for measuring residual stress in cutting AISI 1045 steel is conducted. Also, Jiann's model and the improved model are simulated under the same conditions with cutting experiment. The comparisons show that the surface residual stresses predicted by the improved model is closer to the experimental results than the results predicted by Jiann's model.
Improved analytical model for residual stress prediction in orthogonal cutting
NASA Astrophysics Data System (ADS)
Qi, Zhaoxu; Li, Bin; Xiong, Liangshan
2014-09-01
The analytical model of residual stress in orthogonal cutting proposed by Jiann is an important tool for residual stress prediction in orthogonal cutting. In application of the model, a problem of low precision of the surface residual stress prediction is found. By theoretical analysis, several shortages of Jiann's model are picked out, including: inappropriate boundary conditions, unreasonable calculation method of thermal stress, ignorance of stress constraint and cyclic loading algorithm. These shortages may directly lead to the low precision of the surface residual stress prediction. To eliminate these shortages and make the prediction more accurate, an improved model is proposed. In this model, a new contact boundary condition between tool and workpiece is used to make it in accord with the real cutting process; an improved calculation method of thermal stress is adopted; a stress constraint is added according to the volume-constancy of plastic deformation; and the accumulative effect of the stresses during cyclic loading is considered. At last, an experiment for measuring residual stress in cutting AISI 1045 steel is conducted. Also, Jiann's model and the improved model are simulated under the same conditions with cutting experiment. The comparisons show that the surface residual stresses predicted by the improved model is closer to the experimental results than the results predicted by Jiann's model.
Hyvärinen, Aapo; Hirayama, Jun-ichiro; Kiviniemi, Vesa; Kawanabe, Motoaki
2016-03-01
In many multivariate time series, the correlation structure is nonstationary, that is, it changes over time. The correlation structure may also change as a function of other cofactors, for example, the identity of the subject in biomedical data. A fundamental approach for the analysis of such data is to estimate the correlation structure (connectivities) separately in short time windows or for different subjects and use existing machine learning methods, such as principal component analysis (PCA), to summarize or visualize the changes in connectivity. However, the visualization of such a straightforward PCA is problematic because the ensuing connectivity patterns are much more complex objects than, say, spatial patterns. Here, we develop a new framework for analyzing variability in connectivities using the PCA approach as the starting point. First, we show how to analyze and visualize the principal components of connectivity matrices by a tailor-made rank-two matrix approximation in which we use the outer product of two orthogonal vectors. This leads to a new kind of transformation of eigenvectors that is particularly suited for this purpose and often enables interpretation of the principal component as connectivity between two groups of variables. Second, we show how to incorporate the orthogonality and the rank-two constraint in the estimation of PCA itself to improve the results. We further provide an interpretation of these methods in terms of estimation of a probabilistic generative model related to blind separation of dependent sources. Experiments on brain imaging data give very promising results. PMID:26735746
Statistical benchmarking for orthogonal electrostatic quantum dot qubit devices
NASA Astrophysics Data System (ADS)
Gamble, John; Frees, Adam; Friesen, Mark; Coppersmith, S. N.
2014-03-01
Quantum dots in semiconductor systems have emerged as attractive candidates for the implementation of quantum information processors because of the promise of scalability, manipulability, and integration with existing classical electronics. A limitation in current devices is that the electrostatic gates used for qubit manipulation exhibit strong cross-capacitance, presenting a barrier for practical scale-up. Here, we introduce a statistical framework for making precise the notion of orthogonality. We apply our method to analyze recently implemented designs at the University of Wisconsin-Madison that exhibit much increased orthogonal control than was previously possible. We then use our statistical modeling to future device designs, providing practical guidelines for devices to have robust control properties. Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy Nuclear Security Administration under contract DE-AC04-94AL85000. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressly or implied, of the US Government. This work was supported in part by the Laboratory Directed Research and Development program at Sandia National Laboratories, by ARO (W911NF-12-0607), and by the United States Department of Defense.
Empirical Orthogonal Functions of the Australian Regional Ionosphere
NASA Astrophysics Data System (ADS)
Bouya, Z.; Terkildsen, M.; Francis, M.
2011-12-01
Understanding the natural background variability of the ionosphere is required for investigations of the ionospheric response to external perturbations. Decomposition of ionospheric observations into a set of spatiotemporal structures allows the separation of the anomalous response of the ionosphere from the background variability. This paper presents preliminary results of the analysis of Australian regional Total Electron Content (TEC) using an Empirical Orthogonal Function (EOF) technique to distinguish the variability of the ionosphere caused by the solar and geomagnetic activity. TEC data at evenly distributed grid points was estimated using the Spherical Cap Harmonic Analysis (SCHA) method, applied to GPS data collected from the Australian Regional GPS Network (ARGN). This approach yields more detailed maps of regional TEC variation than can be obtained using global models. EOF analysis was used to decompose the TEC data set into a series of orthogonal Eigenfunction (EOF base function) and associated coefficients. The base function represents the variation in TEC with latitude and longitude. The coefficients represent the variation with time. Analysis of a year worth of data from 2010 yielded the following results: The first 5 EOFs describe 95% of the total TEC variance. The equatorial zone dominates in the spatial structure of the first EOF. This mode has a distinct diurnal and seasonal evolution its proportion is ~65% and highly correlates with the solar activity. Keywords: EOF, Equatorial Anomaly, Regional, SCHA, TEC, GPS, Australia .
Improvement in Lesion Detection with Whole – Body Oncologic TOF - PET
El Fakhri, Georges; Surti, Suleman; Trott, Cathryn M.; Scheuermann, Joshua; Karp, Joel S.
2011-01-01
Time of Flight (TOF) PET has great potential in whole-body oncological applications and recent work has demonstrated qualitatively in patient studies the improvement that can be achieved in lesion visibility. The aim of this work was to objectively quantify the improvement in lesion detectability that can be achieved in pulmonary and hepatic lesions with whole body FDG TOF-PET in a cohort of 100 patients as a function of patient body mass index (BMI), lesion location and contrast, and scanning time. Methods 100 patients with BMIs ranging from 16 to 45 were included in this study. Artificial 1-cm spherical lesions were imaged separately in air at variable locations of each patient’s lung and liver, appropriately attenuated and incorporated in the patient listmode data with four different lesion-to-background contrast ranges. The fused studies with artificial lesion present or absent were reconstructed using a listmode, un-relaxed OSEM with chronologically ordered subsets and a Gaussian TOF kernel for TOF reconstruction. Conditions were compared on the basis of performance of a 3-channel Hotelling observer (CHO-SNR) in detecting the presence of a sphere of unknown size on an anatomic background while modeling observer noise. Results TOF-PET yielded an improvement in lesion detection performance (ratio of CHO-SNR) over non-TOF PET of 8.3% in the liver and 15.1% in the lungs. The improvement in all lesions was 20.3, 12.0, 9.2 and 7.5% for mean contrast values of 2.0:1, 3.2:1, 4.4:1, and 5.7:1, respectively. Furthermore, this improvement was 9.8% in patients with BMI<30 and 11.1% in patients with BMI>30. Performance plateaued faster as a function of number of iterations with TOF than non-TOF. Conclusion TOF-PET yielded a significant improvement in lesion detection in oncologic studies over all contrasts and BMIs that was greater for lower lesion contrast. This improvement was achieved without compromising other aspects of PET imaging. PMID:21321265
Developing integrated TOF-SIMS/MALDI IMS system in studying biological systems
NASA Astrophysics Data System (ADS)
Wu, Ligang
Using imaging mass spectrometry (IMS) techniques (including TOF-SIMS and MALDI IMS) to study biological systems is a relatively new concept and quickly gained popularity in recent years. Imaging mass spectrometry is a discovery technology that utilizes a focused ion beam or laser beam to desorb ions from sample surface. By detecting the desorbed ions, the chemical distributions and biological changes of a sample surface can be analyzed. These techniques offer a new analytical imaging approach to investigate biological processes at the cellular and tissue level. In this research, a novel integrated TOF-SIMS/MALDI IMS system as well as IMS based biological-sample-preparation techniques and data-reduction methods are developed. We then demonstrate the power of these techniques in studying different biological systems, including monosaccharides isomers, human breast cancer cell lines, mouse embryo tissues and mouse kidney sections. Using TOF-SIMS and statistical analysis methods, seven monosaccharide isomers are fully differentiated by analyzing their characteristic spectral pattern. In addition, a deep understanding of the fragmentation pathway of these isomers under ion bombardment is gained. In an application of TOF-SIMS to the differentiation of three human breast cancer cell lines, MCF-7, T47D, and MDA-MB-231, we show that principal component analysis (PCA) data reduction of TOF-SIMS spectra can differentiate cellular compartments (cytosol, nuclear and particulate) within the cell types, as well as homogenates from among the three cell lines. In a tissue-specific application, we extend the analytical capabilities of TOF-SIMS and PCA by imaging and differentiating Formalin-fixed paraffin-embedded (FFPE) mouse embryo tissues. We demonstrate reproducible differentiation of six tissue types based on the remaining small molecules after paraffin-embedding and the fragments of the cellular proteins. In a unique study of fresh frozen mouse kidney tissues, both TOF
Evaluation of ice-tea quality by DART-TOF/MS.
Rajchl, Aleš; Prchalová, Jana; Kružík, Vojtěch; Ševčík, Rudolf; Čížková, Helena
2015-11-01
DART (Direct Analysis in Real Time) coupled with Time-of-Flight Mass Spectrometry (TOF/MS) has been used for analyses of ice-teas. The article focuses on quality and authenticity of ice-teas as one of the most important tea-based products on the market. Twenty-one samples of ice-teas (black and green) were analysed. Selected compounds of ice-teas were determined: theobromine, caffeine, total phenolic compounds, total soluble solids, total amino acid concentration, preservatives and saccharides were determined. Fingerprints of DART-TOF/MS spectra were used for comprehensive assessment of the ice-tea samples. The DART-TOF/MS method was used for monitoring the following compounds: citric acid, caffeine, saccharides, artificial sweeteners (saccharin, acesulphame K), and preservatives (sorbic and benzoic acid), phosphoric acid and phenolic compounds. The measured data were subjected to a principal components analysis. The HPLC and DART-TOF/MS methods were compared in terms of determination of selected compounds (caffeine, benzoic acid, sorbic acid and saccharides) in the ice-teas. The DART-TOF/MS technique seems to be a suitable method for fast screening, testing quality and authenticity of tea-based products. PMID:26505766
[Utility of MALDI-TOF MS for the identification of anaerobic bacteria].
Zárate, Mariela S; Romano, Vanesa; Nievas, Jimena; Smayevsky, Jorgelina
2014-01-01
The analysis by MALDI-TOF MS (Matrix-assited laser desorption/ionization time-of-flight mass spectrometry) has become a reference method for the identification of microorganisms in Clinical Microbiology. However, data on some groups of microorganisms are still controversial. The aim of this study is to determine the utility of MALDI-TOF MS for the identification of clinical isolates of anaerobic bacteria. One-hundred and six anaerobic bacteria isolates were analyzed by MALDI-TOF MS and by conventional biochemical tests. In those cases where identification by conventional methodology was not applicable or in the face of discordance between sequencing methodologies, 16 S rRNA gene sequence analysis was performed. The conventional method and MALDI-TOF MS agreed at genus and species level by 95.3 %. Concordance in gram-negative bacilli was 91.4% and 100% among gram-positive bacilli; there was also concordance both in the 8 isolates studied in gram-positive cocci and in the single gram-negative cocci included. The data obtained in this study demonstrate that MALDI-TOF MS offers the possibility of adequate identification of anaerobic bacteria. PMID:25011591
Nonresonant Laser SNMS and TOF SIMS analysis of sub-μm structures
NASA Astrophysics Data System (ADS)
Kollmer, F.; Bourdos, N.; Kamischke, R.; Benninghoven, A.
2003-01-01
We determined and compared the capabilities of TOF-SIMS and Laser-SNMS for chemical mapping of sub-μm structures by investigating different inorganic and organic samples. For our investigations we used a combined TOF-SIMS/Laser-SNMS instrument, equipped with a focused (<80 nm) Ga +-LMIS and an excimer laser for nonresonant postionization of sputtered neutrals. For 16 monoelemental metal samples we determined useful yields up to 10 -2 (Laser-SNMS) and 10 -3 (TOF-SIMS), respectively. The high yields allow chemical characterization of metal oxide particles, such as Al 2O 3, Fe 3O 4 and CeO 2 with diameters well below the spot size of the primary ion beam, as well as the detection and identification of metal loads on nanotips and semiquantitative elemental mapping of catalysts. Compared with TOF-SIMS, Laser-SNMS features a number of advantages for elemental analysis: higher and more uniform yields, i.e. sensitivities, ease of quantification because of a drastically reduced matrix effect, and compatibility with extreme surface topographies, since sputtered neutrals are not affected by distortions of the extraction field. Laser-SNMS and TOF-SIMS results for phase separated phospholipid/protein LB monolayers on Au substrates demonstrate the capabilities of these techniques for molecular surface mapping.
Investigation of natural dyes and ancient textiles from korea using TOF-SIMS
NASA Astrophysics Data System (ADS)
Lee, Yeonhee; Lee, Jihye; Kim, Youngsoo; Choi, Seokchan; Ham, Seung Wook; Kim, Kang-Jin
2008-12-01
The identification of the colorants used on ancient textiles provides a historical pathway to the understanding of the processes associated with one of the oldest of chemical technologies, namely textile dyeing. In this paper, time-of-flight secondary ion mass spectrometry (TOF-SIMS) was used to detect dyes on textiles avoiding the time-consuming and destructive extraction procedures necessary for the spectrophotometric and chromatographic methods previously used. The plant dyes investigated belong to a variety of chemical groups, which include curcumin, crocin, carthamin, purpurin, alizarin, brazilin, shikonin, and indigo. Reference textile samples were prepared with dye extracts of plants and were characterized by TOF-SIMS. TOF-SIMS spectra for the dyed textiles showed element ions from metallic mordants, specific fragment ions, and molecular ions from organic dyes. Remnant dyes on excavated textiles have also been identified using TOF-SIMS. The ancient textile sample showed the presence of indigo clearly, although the fiber itself had degraded badly. From the results, it was concluded that most of plant dyes can be identified with TOF-SIMS and it is a very promising technique for the archaeology field.
Abreu, Lucas M; Moreira, Gláucia M; Ferreira, Douglas; Rodrigues-Filho, Edson; Pfenning, Ludwig H
2014-12-01
We assessed the species diversity among 45 strains of Clonostachys from different substrates and localities in Brazil using molecular phylogenetics, and compared the results with the phenotypic classification of strains obtained from matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Phylogenetic analyses were based on beta tubulin (Tub), ITS-LSU rDNA, and a combined Tub-ITS DNA dataset. MALDI-TOF MS analyses were performed using intact conidia and conidiophores of strains cultivated on oatmeal agar and 4% malt extract agar. Six known species were identified: Clonostachys byssicola, Clonostachys candelabrum, Clonostachys pseudochroleuca, Clonostachys rhizophaga, Clonostachys rogersoniana, and Clonostachys rosea. Two clades and two singleton lineages did not correspond to known species represented in the reference DNA dataset and were identified as Clonostachys sp. 1-4. Multivariate cluster analyses of MALDI-TOF MS data classified the strains into eight clusters and three singletons, corresponding to the ten identified species plus one additional cluster containing two strains of C. rogersoniana that split from the other co-specific strains. The consistent results of MALDI-TOF MS supported the identification of strains assigned to C. byssicola and C. pseudochroleuca, which did not form well supported clades in all phylogenetic analyses, but formed distinct clusters in the MALDI-TOF dendrograms. PMID:25457948
Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes
NASA Astrophysics Data System (ADS)
Petkovic, Marijana; Petrovic, Biljana; Savic, Jasmina; Bugarcic, Zivadin D.; Dimitric-Markovic, Jasmina; Momic, Tatjana; Vasic, Vesna
2010-02-01
Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a suitable method for the analysis of inorganic and organic compounds and biomolecules. This makes MALDI-TOF MS convenient for monitoring the interaction of metallo-drugs with biomolecules. Results presented in this manuscript demonstrate that flavonoids such as apigenin, kaempferol and luteolin are suitable for MALDI-TOF MS analysis of Pt(II), Pd(II), Pt(IV) and Ru(III) complexes, giving different signal-to-noise ratios of the analyte peak. The MALDI-TOF mass spectra of inorganic complexes acquired with these flavonoid matrices are easy to interpret and have some advantages over the application of other commonly used matrices: a low number of matrix peaks are detectable and the coordinative metal-ligand bond is, in most cases, preserved. On the other hand, flavonoids do not act as typical matrices, as their excess is not required for the acquisition of MALDI-TOF mass spectra of inorganic complexes.
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
Schultz, Peter G.; Wang, Lei; Anderson, John Christopher; Chin, Jason W.; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Stephen William; Zhang, Zhiwen
2015-10-20
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
Methods and composition for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
Schultz, Peter G.; Wang, Lei; Anderson, John Christopher; Chin, Jason W.; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Stephen William; Zhang, Zhiwen
2012-05-08
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
Schultz, Peter; Wang, Lei; Anderson, John Christopher; Chin, Jason; Liu, David R.; Magliery, Thomas J.; Meggers, Eric L.; Mehl, Ryan Aaron; Pastrnak, Miro; Santoro, Stephen William; Zhang, Zhiwen
2006-08-01
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.
Technology Transfer Automated Retrieval System (TEKTRAN)
Spectral fingerprints were acquired for Ruby Red grapefruit using direct injection-electrospray ionization with time-of-flight and ion trap mass spectrometry (DI-ESI-TOF-MS and DI-ESI-IT-MS). Rio Red grapefruits were harvested 3 times a year (early, mid, and late harvests) in 2005 and 2006 from con...
Design and content determination of nimesulide injectable formulation.
Luo, Lei; Tao, Wanfu; Bourkaib, Nadia; Luo, Yonghuang
2015-07-01
The aim of the present study was to formulate Nimesulide inject able solution, establish a method for content determination, and accumulate data for registration of a new Nimesulide formulation. The optimal Nimesulide inject able formulation was determined based on the results of single factor test and orthogonal test. Moreover, clarity, stability, pH, content and related substances of Nimesulide were used as the main study indicators. The content of Nimesulide in inject able solution was determined by the high performance liquid chromatography (HPLC) method. The mobile phase consisted of V (methanol): V (potassium dihydrogen phosphate, pH 4.2)=60:40 at a flow rate of 1.0 mL/min. The detection was carried out with UV detector (λ(max) =254 nm) under a column temperature of 25°C and an injection volume of 20μL. The optimal inject able formulation was 4% Nimesulide, 4% ethanolamine, 0.1% L-cysteine, 0.01% EDTA-2Na, a suitable amount of lactic acid and water for injection. Nimesulide detection limits range from 20 to 80 μg/mL with a correlation coefficient of 0.9995 and high average recovery 99.91% (RSD=0.06%). In conclusion, the formulation was suitable for Nimesulide inject able form, and the determination method was simple, sensitive and accurate. Therefore, the Nimesulide inject able formulation can be used for industrial production and effectively controlled. PMID:26142510
Chang, Kai Lun; Ho, Paul C.
2014-01-01
Findings from epidemiology, preclinical and clinical studies indicate that consumption of coffee could have beneficial effects against dementia and Alzheimer’s disease (AD). The benefits appear to come from caffeinated coffee, but not decaffeinated coffee or pure caffeine itself. Therefore, the objective of this study was to use metabolomics approach to delineate the discriminant metabolites between caffeinated and decaffeinated coffee, which could have contributed to the observed therapeutic benefits. Gas chromatography time-of-flight mass spectrometry (GC-TOF-MS)-based metabolomics approach was employed to characterize the metabolic differences between caffeinated and decaffeinated coffee. Orthogonal partial least squares discriminant analysis (OPLS-DA) showed distinct separation between the two types of coffee (cumulative Q2 = 0.998). A total of 69 discriminant metabolites were identified based on the OPLS-DA model, with 37 and 32 metabolites detected to be higher in caffeinated and decaffeinated coffee, respectively. These metabolites include several benzoate and cinnamate-derived phenolic compounds, organic acids, sugar, fatty acids, and amino acids. Our study successfully established GC-TOF-MS based metabolomics approach as a highly robust tool in discriminant analysis between caffeinated and decaffeinated coffee samples. Discriminant metabolites identified in this study are biologically relevant and provide valuable insights into therapeutic research of coffee against AD. Our data also hint at possible involvement of gut microbial metabolism to enhance therapeutic potential of coffee components, which represents an interesting area for future research. PMID:25098597
Automated Gain Control Ion Funnel Trap for Orthogonal Time-of-Flight Mass Spectrometry
Ibrahim, Yehia M.; Belov, Mikhail E.; Liyu, Andrei V.; Smith, Richard D.
2009-01-01
Time-of-flight mass spectrometry (TOF MS) is increasingly used in proteomics research. Herein, we report on the development and characterization of a TOF MS instrument with improved sensitivity equipped with an electrodynamic ion funnel trap (IFT) that employs an automated gain control (AGC) capability. The IFT-TOF MS was coupled to a reversed-phase capillary liquid chromatography (RPLC) separation and evaluated in experiments with complex proteolytic digests. When applied to a global tryptic digest of Shewanella oneidensis proteins, an order-of-magnitude increase in sensitivity compared to that of the conventional continuous mode of operation was achieved due to efficient ion accumulation prior to TOF MS analysis. As a result of this sensitivity improvement and related improvement in mass measurement accuracy, the number of unique peptides identified in the AGC-IFT mode was 5-fold greater than that obtained in the continuous mode. PMID:18512944
Futatani, S.; Bos, W.J.T.; Del-Castillo-Negrete, Diego B; Schneider, Kai; Benkadda, S.; Farge, Marie
2011-01-01
We assess two techniques for extracting coherent vortices out of turbulent flows: the wavelet based Coherent Vorticity Extraction (CVE) and the Proper Orthogonal Decomposition (POD). The former decomposes the flow field into an orthogonal wavelet representation and subsequent thresholding of the coefficients allows one to split the flow into organized coherent vortices with non-Gaussian statistics and an incoherent random part which is structureless. POD is based on the singular value decomposition and decomposes the flow into basis functions which are optimal with respect to the retained energy for the ensemble average. Both techniques are applied to direct numerical simulation data of two-dimensional drift-wave turbulence governed by Hasegawa Wakatani equation, considering two limit cases: the quasi-hydrodynamic and the quasi-adiabatic regimes. The results are compared in terms of compression rate, retained energy, retained enstrophy and retained radial flux, together with the enstrophy spectrum and higher order statistics. (c) 2010 Published by Elsevier Masson SAS on behalf of Academie des sciences.
Marchetti-Deschmann, Martina; Stephan, Christopher; Häubl, Georg; Allmaier, Günter; Krska, Rudolf; Cvak, Barbara
2016-07-15
The increasing number of bioconjugates used for bioanalytical purposes and in pharmaceutical industries has led to an increasing demand for robust quality control of products derived from covalently linking small molecules to proteins. Here we report, for the first time, a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF)-based method to determine the quantity and location of the hapten zearalenone (ZEN) introduced to the carrier protein conalbumin (Con). This bioconjugate is of special interest because of its application in lateral flow immunoassays commercially available for fast testing of food and feed for the presence of ZEN, a common contaminant of all major cereal grains worldwide. Mass spectrometry (MS) analysis of the intact protein turned out to be highly reproducible allowing for the determination of the average hapten load of the carrier protein. In that way an easy and fast method to screen for changes in ZEN load after bioconjugate synthesis was established. For a more detailed hapten load characterization, measurements at the peptide level were of importance. Systematic studies, implementing post-source decay (PSD) and high- and low-energy collision-induced dissociation (CID), showed characteristic fragmentation pattern for three model peptides carrying between one and three lysines (the primary target for the ZEN modification) besides other, less obvious modification sites (serine, arginine and the N-terminus). By this, indicative reporter ions (m/z 203 and 316) and neutral losses (Δm/z 373 and 317) for the ZEN modification in general, plus immonium ions (m/z 87, 142 and 159) for the lysine modification in particular were identified. Based on these findings, proteolytic peptides, tentatively assigned to be modified, were unequivocally confirmed to be affected by bioconjugation. For a protein carrying on average only 2-3 modifications per molecule 29 Lys out of 59 potential modifications sites were actually modified
NASA Astrophysics Data System (ADS)
Thomas, T.; Suyker, A.; Burba, G. G.; Billesbach, D.
2014-12-01
The eddy covariance method for estimating fluxes of trace gases, energy and momentum in the constant flux layer above a plant canopy fundamentally relies on accurate measurements of the vertical wind speed. This wind speed is typically measured using a three dimensional ultrasonic anemometer. These anemometers incorporate designs with transducer sets that are aligned either orthogonally or non-orthogonally. Previous studies comparing the two designs suggest differences in measured 3D wind speed components, in particular vertical wind speed, from the non-orthogonal transducer relative to the orthogonal design. These differences, attributed to additional flow distortion caused by the non-orthogonal transducer arrangement, directly affect fluxes of trace gases, energy and momentum. A field experiment is being conducted over a rain-fed soybean field at the AmeriFlux site (US-Ne3) near Mead, Nebraska. In this study, ultrasonic anemometers featuring orthogonal transducer sets (ATI Vx Probe) and non-orthogonal transducer sets (Gill R3-100) collect high frequency wind vector and sonic temperature data. Sensible heat and momentum fluxes and other key sonic performance data are evaluated based on environmental parameters including wind speed, wind direction, temperature, and angle of attack. Preliminary field experiment results are presented.
Application of neural networks with orthogonal activation functions in control of dynamical systems
NASA Astrophysics Data System (ADS)
Nikolić, Saša S.; Antić, Dragan S.; Milojković, Marko T.; Milovanović, Miroslav B.; Perić, Staniša Lj.; Mitić, Darko B.
2016-04-01
In this article, we present a new method for the synthesis of almost and quasi-orthogonal polynomials of arbitrary order. Filters designed on the bases of these functions are generators of generalised quasi-orthogonal signals for which we derived and presented necessary mathematical background. Based on theoretical results, we designed and practically implemented generalised first-order (k = 1) quasi-orthogonal filter and proved its quasi-orthogonality via performed experiments. Designed filters can be applied in many scientific areas. In this article, generated functions were successfully implemented in Nonlinear Auto Regressive eXogenous (NARX) neural network as activation functions. One practical application of the designed orthogonal neural network is demonstrated through the example of control of the complex technical non-linear system - laboratory magnetic levitation system. Obtained results were compared with neural networks with standard activation functions and orthogonal functions of trigonometric shape. The proposed network demonstrated superiority over existing solutions in the sense of system performances.
One method for calculating flux-MMF relationship of orthogonal-core
Tajima, Katsubumi; Kaga, Akio; Anazawa, Yoshihisa . Dept. of Electrical and Electronics Engineering); Ichinokura, Osamu . Dept. of Electrical Engineering)
1993-11-01
The orthogonal-core has various applications, e.g. as a variable inductor, a parametric transformer and a DC-AC converter. This paper describes one method for calculating the flux-MMF relationship of an orthogonal-core. The calculation is based on a 3-dimensional magnetic circuit model of the orthogonal-core. The model is derived by dividing the orthogonal-core, inclusive of the surrounding region, into elements comprising a 3-dimensional magnetic circuit. Using this model, the authors can compute the flux-MMF relationship of the orthogonal core with arbitrary dimensions from the B-H characteristic of the core material. The calculation method presented here is useful for optimum design of devices using an orthogonal-core.
TOF-Bρ mass measurements of very exotic nuclides for astrophysical calculations at the NSCL
NASA Astrophysics Data System (ADS)
Matoš, M.; Estrade, A.; Amthor, M.; Aprahamian, A.; Bazin, D.; Becerril, A.; Elliot, T.; Galaviz, D.; Gade, A.; Gupta, S.; Lorusso, G.; Montes, F.; Pereira, J.; Portillo, M.; Rogers, A. M.; Schatz, H.; Shapira, D.; Smith, E.; Stolz, A.; Wallace, M.
2008-01-01
Atomic masses play a crucial role in many nuclear astrophysics calculations. The lack of experimental values for relevant exotic nuclides triggered a rapid development of new mass measurement devices around the world. The time-of-flight (TOF) mass measurements offer a complementary technique to the most precise one, Penning trap measurements (Blaum 2006 Phys. Rep. 425 1), the latter being limited by the rate and half-lives of the ions of interest. The NSCL facility provides a well-suited infrastructure for the TOF mass measurements of very exotic nuclei. At this facility, we have recently implemented a TOF-Bρ technique and performed mass measurements of neutron-rich nuclides in the Fe region, important for r-process calculations and for calculations of processes occurring in the crust of accreting neutron stars.
TOF Electron Energy Analyzer for Spin and Angular Resolved Photoemission Spectroscopy
Lebedev, Gennadi; Jozwiak, Chris; Andresen, Nord; Lanzara, Alessandra; Hussain, Zahid
2008-07-09
Current pulsed laser and synchrotron x-ray sources provide new opportunities for Time-Of- Flight (TOF) based photoemission spectroscopy to increase photoelectron energy resolution and efficiency compared to current standard techniques. The principals of photoelectron timing front formation, temporal aberration minimization, and optimization of electron beam transmission are presented. We have developed these concepts into a high resolution Electron Optical Scheme (EOS) of a TOF Electron Energy Analyzer (TOF-EEA) for photoemission spectroscopy. The EOS of the analyzer includes an electrostatic objective lens, three columns of transport lenses and a 90 degree energy band pass filter (BPF). The analyzer has two modes of operation: Spectrometer Mode (SM) with straight passage of electrons through the EOS undeflected by the BPF, allowing the entire spectrum to be measured, and Monochromator Mode (MM) in which the BPF defines a certain energy window inside the scope of the electron energy spectrum.
[Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification].
Relloso, María S; Nievas, Jimena; Fares Taie, Santiago; Farquharson, Victoria; Mujica, María T; Romano, Vanesa; Zarate, Mariela S; Smayevsky, Jorgelina
2015-01-01
The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification. PMID:25882136
Schaumann, Reiner; Knoop, Nicolas; Genzel, Gelimer H; Losensky, Kevin; Rosenkranz, Christiane; Stîngu, Catalina S; Schellenberger, Wolfgang; Rodloff, Arne C; Eschrich, Klaus
2013-01-01
Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli. PMID:23919091
Schaumann, Reiner; Knoop, Nicolas; Genzel, Gelimer H; Losensky, Kevin; Rosenkranz, Christiane; Stîngu, Catalina S; Schellenberger, Wolfgang; Rodloff, Arne C; Eschrich, Klaus
2013-01-01
Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli. PMID:23919091
Rapid identification of acetic acid bacteria using MALDI-TOF mass spectrometry fingerprinting.
Andrés-Barrao, Cristina; Benagli, Cinzia; Chappuis, Malou; Ortega Pérez, Ruben; Tonolla, Mauro; Barja, François
2013-03-01
Acetic acid bacteria (AAB) are widespread microorganisms characterized by their ability to transform alcohols and sugar-alcohols into their corresponding organic acids. The suitability of matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF MS) for the identification of cultured AAB involved in the industrial production of vinegar was evaluated on 64 reference strains from the genera Acetobacter, Gluconacetobacter and Gluconobacter. Analysis of MS spectra obtained from single colonies of these strains confirmed their basic classification based on comparative 16S rRNA gene sequence analysis. MALDI-TOF analyses of isolates from vinegar cross-checked by comparative sequence analysis of 16S rRNA gene fragments allowed AAB to be identified, and it was possible to differentiate them from mixed cultures and non-AAB. The results showed that MALDI-TOF MS analysis was a rapid and reliable method for the clustering and identification of AAB species. PMID:23182036
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
Anderson, J. Christopher; Wu, Ning; Santoro, Stephen; Schultz, Peter G.
2009-08-18
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
Anderson, J. Christopher; Wu, Ning; Santoro, Stephen; Schultz, Peter G.
2009-12-29
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
Anderson, J. Christopher; Wu, Ning; Santoro, Stephen; Schultz, Peter G.
2011-10-04
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
Anderson, J. Christopher; Wu, Ning; Santoro, Stephen; Schultz, Peter G
2014-03-11
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.
Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest.
Bastia, Deepak; Srivastava, Pankaj; Zaman, Shamsu; Choudhury, Malay; Mohanty, Bidyut K; Bacal, Julien; Langston, Lance D; Pasero, Philippe; O'Donnell, Michael E
2016-06-28
Several important physiological transactions, including control of replicative life span (RLS), prevention of collision between replication and transcription, and cellular differentiation, require programmed replication fork arrest (PFA). However, a general mechanism of PFA has remained elusive. We previously showed that the Tof1-Csm3 fork protection complex is essential for PFA by antagonizing the Rrm3 helicase that displaces nonhistone protein barriers that impede fork progression. Here we show that mutations of Dbf4-dependent kinase (DDK) of Saccharomyces cerevisiae, but not other DNA replication factors, greatly reduced PFA at replication fork barriers in the spacer regions of the ribosomal DNA array. A key target of DDK is the mini chromosome maintenance (Mcm) 2-7 complex, which is known to require phosphorylation by DDK to form an active CMG [Cdc45 (cell division cycle gene 45), Mcm2-7, GINS (Go, Ichi, Ni, and San)] helicase. In vivo experiments showed that mutational inactivation of DDK caused release of Tof1 from the chromatin fractions. In vitro binding experiments confirmed that CMG and/or Mcm2-7 had to be phosphorylated for binding to phospho-Tof1-Csm3 but not to its dephosphorylated form. Suppressor mutations that bypass the requirement for Mcm2-7 phosphorylation by DDK restored PFA in the absence of the kinase. Retention of Tof1 in the chromatin fraction and PFA in vivo was promoted by the suppressor mcm5-bob1, which bypassed DDK requirement, indicating that under this condition a kinase other than DDK catalyzed the phosphorylation of Tof1. We propose that phosphorylation regulates the recruitment and retention of Tof1-Csm3 by the replisome and that this complex antagonizes the Rrm3 helicase, thereby promoting PFA, by preserving the integrity of the Fob1-Ter complex. PMID:27298353
Ensemble forecast of typhoon generated by orthogonal conditional nonlinear optimal perturbations
NASA Astrophysics Data System (ADS)
Huo, Zhenhua; Duan, Wansuo; Zhou, Feifan
2016-04-01
Orthogonal conditional nonlinear optimal perturbations (CNOPs) are the initial perturbations that have the largest impact on the forecast results in orthogonal subspaces of the initial perturbation space. Previous studies demonstrate the successful application of orthogonal CNOPs in ensemble forecasting. And further analysis indicates that orthogonal CNOPs may be more adapt to the prediction of strong events, among which typhoon events occur in the tropical or subtropical areas where the diabatic physical processes is very important and has strong nonlinear behavior. For these reasons, this paper focuses on the application of orthogonal CNOPs in ensemble forecast of typhoon. In this study, orthogonal CNOPs, orthogonal singular vectors (SVs), bred vectors (BVs) and random perturbations (RPs) are applied for typhoon ensemble forecasts using MM5 model. The results show that, for typhoons Matsa in 2005 and Sepat in 2007, ensemble forecasts generated by orthogonal CNOPs greatly improve the control forecast, successfully predicts the landing location of Matsa, and gives the warning information of the landing of Sepat. In detail, for the ensemble mean associated with orthogonal CNOPs, the averaging track forecast error over 5 days is decreased by 45.58 km for Matsa and 87.8 km for Sepat, compared with control forecast. However, ensemble forecasts generated by other three methods could not successfully predict the landing location of Matsa and give the warning information of the landing of Sepat. Compared with orthogonal SVs, BVs and RPs, ensemble forecasts generated by orthogonal CNOPs corresponds to the largest ensemble spread, improves the control forecast at the largest extent, and best samples the distribution of initial analysis errors. All these results show that orthogonal CNOPs may provide another useful technique for ensemble forecast of typhoon.
Technology Transfer Automated Retrieval System (TEKTRAN)
We have measured the relative abundance of the B-subunits and mRNA transcripts of two Stx2 subtypes present in Shiga toxin-producing Escherichia coli (STEC) O157:H- strain E32511 using matrix-assisted laser desorption/ionization time-of-flight-time-of-flight tandem mass spectrometry (MALDI-TOF-TOF-M...
Technology Transfer Automated Retrieval System (TEKTRAN)
A method has been developed to identify the alpha-subunit of shiga toxin 2 (alpha-Stx2) from Escherichia coli O157:H7 using matrix-assisted laser desorption/ionization time-of-flight-time-of-flight tandem mass spectrometry (MALDI-TOF-TOF-MS/MS) and top-down proteomics using web-based software develo...
Technology Transfer Automated Retrieval System (TEKTRAN)
The disulfide-intact and disulfide-reduced beta-subunit of Shiga toxin 2 (beta-Stx2) from Escherichia coli O157:H7 (strain EDL933) has been identified by matrix-assisted laser desorption/ionization time-of-flight-time-of-flight tandem mass spectrometry (MALDI-TOF-TOF-MS/MS) and top-down proteomic an...
van Prehn, Joffrey; van Veen, Suzanne Q; Schelfaut, Jacqueline J G; Wessels, Els
2016-05-01
We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used. PMID:26971637
Time expanding multihit TDC for the BELLE TOF detector at the KEK B-factory
Varner, G.; Kichimi, H.; Yamaguchi, H.
1997-12-31
Utilizing a time expansion technique, a multihit TDC has been developed for readout of the BELLE TOF detector at the KEK B-Factory. Time digitization consists of three steps: tagging an input signal with respect to a beam collision synchronized reference clock, expansion of this time interval, and readout by a conventional multihit TDC. Using a time expansion factor of 20 and a multihit TDC with a 500 ps LSB, this system provides a precision TOF measurement of 25 ps LSB, {approximately}20 ps resolution, and with a dead time of less than 1 {mu}s.
Corticotropin, Repository Injection
... age; episodes of symptoms in people who have multiple sclerosis (MS; a disease in which the nerves do ... When corticotropin repository injection is used to treat multiple sclerosis, it is usually injected once a day for ...
Urinary incontinence - injectable implant
Injectable implants are injections of material into the urethra to help control urine leakage ( urinary incontinence ) caused by a ... into the tissue next to the sphincter. The implant procedure is usually done in the hospital. Or ...
Calcitonin salmon injection is used to treat osteoporosis in postmenopausal women. Osteoporosis is a disease that causes bones to weaken and break more easily. Calcitonin salmon injection is also used to treat Paget's disease ...
Botox® Cosmetic ... OnabotulinumtoxinA injection (Botox, Botox Cosmetic) is used to treat a number of conditions.OnabotulinumtoxinA injection (Botox) is used to relieve the symptoms of cervical dystonia ( ...
... this page: //medlineplus.gov/ency/article/007633.htm Hip joint injection To use the sharing features on this ... injection is a shot of medicine into the hip joint. The medicine helps relieve pain and inflammation. It ...
... severe submental fat ('double chin'; fatty tissue located under the chin). Deoxycholic acid injection is in a ... as a liquid to be injected subcutaneously (just under the skin) by a doctor. Your doctor will ...
(rim a bott' you lye num bee)RimabotulinumtoxinB injection may spread from the area of injection and ... Event Reporting program online (http://www.fda.gov/Safety/MedWatch) or by phone (1-800-332-1088).
Aminocaproic acid injection is used to control bleeding that occurs when blood clots are broken down too quickly. This ... the baby is ready to be born). Aminocaproic acid injection is also used to control bleeding in ...
Deoxycholic acid injection is used to improve the appearance and profile of moderate to severe submental fat ('double chin'; fatty tissue located under the chin). Deoxycholic acid injection is in a class of medications called ...
Sodium Ferric Gluconate Injection
Sodium ferric gluconate injection is used to treat iron-deficiency anemia (a lower than normal number of ... are also receiving the medication epoetin (Epogen, Procrit). Sodium ferric gluconate injection is in a class of ...
Iron dextran injection is used to treat iron-deficiency anemia (a lower than normal number of red blood cells ... treated with iron supplements taken by mouth. Iron dextran injection is in a class of medications called ...
... injection is used to relieve the symptoms of cervical dystonia (spasmodic torticollis; uncontrollable tightening of the neck ... is injected into a muscle, it blocks the nerve signals that cause uncontrollable tightening and movements of ...
State diagram of an orthogonal spin transfer spin valve device
Ye, Li; Wolf, Georg; Pinna, Daniele; Chaves-O'Flynn, Gabriel D.; Kent, Andrew D.
2015-05-21
We present the switching characteristics of a spin-transfer device that incorporates a perpendicularly magnetized spin-polarizing layer with an in-plane magnetized free and fixed magnetic layer, known as an orthogonal spin transfer spin valve device. This device shows clear switching between parallel (P) and antiparallel (AP) resistance states and the reverse transition (AP → P) for both current polarities. Further, hysteretic transitions are shown to occur into a state with a resistance intermediate between that of the P and AP states, again for both current polarities. These unusual spin-transfer switching characteristics can be explained within a simple macrospin model that incorporates thermal fluctuations and considers a spin-polarized current that is tilted with respect to the free layer's plane, due to the presence of the spin-transfer torque from the polarizing layer.
Orthogonally referenced integrated ensemble for navigation and timing
Smith, Stephen Fulton; Moore, James Anthony
2014-04-01
An orthogonally referenced integrated ensemble for navigation and timing includes a dual-polyhedral oscillator array, including an outer sensing array of oscillators and an inner clock array of oscillators situated inside the outer sensing array. The outer sensing array includes a first pair of sensing oscillators situated along a first axis of the outer sensing array, a second pair of sensing oscillators situated along a second axis of the outer sensing array, and a third pair of sensing oscillators situated along a third axis of the outer sensing array. The inner clock array of oscillators includes a first pair of clock oscillators situated along a first axis of the inner clock array, a second pair of clock oscillators situated along a second axis of the inner clock array, and a third pair of clock oscillators situated along a third axis of the inner clock array.
Orthogonally referenced integrated ensemble for navigation and timing
Smith, Stephen Fulton; Moore, James Anthony
2013-02-26
An orthogonally referenced integrated ensemble for navigation and timing includes a dual-polyhedral oscillator array, including an outer sensing array of oscillators and an inner clock array of oscillators situated inside the outer sensing array. The outer sensing array includes a first pair of sensing oscillators situated along a first axis of the outer sensing array, a second pair of sensing oscillators situated along a second axis of the outer sensing array, and a third pair of sensing oscillators situated along a third axis of the outer sensing array. The inner clock array of oscillators includes a first pair of clock oscillators situated along a first axis of the inner clock array, a second pair of clock oscillators situated along a second axis of the inner clock array, and a third pair of clock oscillators situated along a third axis of the inner clock array.