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Sample records for p450scc show anti-leukemia

  1. The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism.

    PubMed

    Slominski, Andrzej; Semak, Igor; Zjawiony, Jordan; Wortsman, Jacobo; Li, Wei; Szczesniewski, Andre; Tuckey, Robert C

    2005-08-01

    We show that cytochrome P450scc (CYP11A1) in either a reconstituted system or in isolated adrenal mitochondria can metabolize vitamin D3. The major products of the reaction with reconstituted enzyme were 20-hydroxycholecalciferol and 20,22-dihydroxycholecalciferol, with yields of 16 and 4%, respectively, of the original vitamin D3 substrate. Trihydroxycholecalciferol was a minor product, likely arising from further metabolism of dihydroxycholecalciferol. Based on NMR analysis and known properties of P450scc we propose that hydroxylation of vitamin D3 by P450scc occurs sequentially and stereospecifically with initial formation of 20(S)-hydroxyvitamin D3. P450scc did not metabolize 25-hydroxyvitamin D3, indicating that modification of C25 protected it against P450scc action. Adrenal mitochondria also metabolized vitamin D3 yielding 10 hydroxyderivatives, with UV spectra typical of vitamin D triene chromophores. Aminogluthimide inhibition showed that the three major metabolites, but not the others, resulted from P450scc action. It therefore appears that non-P450scc enzymes present in the adrenal cortex to some extent contribute to metabolism of vitamin D3. We conclude that purified P450scc in a reconstituted system or P450scc in adrenal mitochondria can add one hydroxyl group to vitamin D3 with subsequent hydroxylation being observed for reconstituted enzyme but not for adrenal mitochondria. Additional vitamin D3 metabolites arise from the action of other enzymes in adrenal mitochondria. These findings appear to define novel metabolic pathways involving vitamin D3 that remain to be characterized.

  2. The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism

    PubMed Central

    Slominski, Andrzej; Semak, Igor; Zjawiony, Jordan; Wortsman, Jacobo; Li, Wei; Szczesniewski, Andre; Tuckey, Robert C.

    2008-01-01

    We show that cytochrome P450scc (CYP11A1) in either a reconstituted system or in isolated adrenal mitochondria can metabolize vitamin D3. The major products of the reaction with reconstituted enzyme were 20-hydroxycholecalciferol and 20,22-dihydroxycholecalciferol, with yields of 16 and 4%, respectively, of the original vitamin D3 substrate. Trihydroxycholecalciferol was a minor product, likely arising from further metabolism of dihydroxycholecalciferol. Based on NMR analysis and known properties of P450scc we propose that hydroxylation of vitamin D3 by P450scc occurs sequentially and stereospecifically with initial formation of 20(S)-hydroxyvitamin D3. P450scc did not metabolize 25-hydroxyvitamin D3, indicating that modification of C25 protected it against P450scc action. Adrenal mitochondria also metabolized vitamin D3 yielding 10 hydroxyderivatives, with UV spectra typical of vitamin D triene chromophores. Aminogluthimide inhibition showed that the three major metabolites, but not the others, resulted from P450scc action. It therefore appears that non-P450scc enzymes present in the adrenal cortex to some extent contribute to metabolism of vitamin D3. We conclude that purified P450scc in a reconstituted system or P450scc in adrenal mitochondria can add one hydroxyl group to vitamin D3 with subsequent hydroxylation being observed for reconstituted enzyme but not for adrenal mitochondria. Additional vitamin D3 metabolites arise from the action of other enzymes in adrenal mitochondria. These findings appear to define novel metabolic pathways involving vitamin D3 that remain to be characterized. PMID:16098191

  3. Resveratrol stimulates cortisol biosynthesis by activating SIRT-dependent deacetylation of P450scc.

    PubMed

    Li, Donghui; Dammer, Eric B; Sewer, Marion B

    2012-07-01

    In the human adrenal cortex, cortisol is synthesized from cholesterol by members of the cytochrome P450 superfamily and hydroxysteroid dehydrogenases. Both the first and last steps of cortisol biosynthesis occur in mitochondria. Based on our previous findings that activation of ACTH signaling changes the ratio of nicotinamide adenine dinucleotide (NAD) phosphate to reduced NAD phosphate in adrenocortical cells, we hypothesized that pyridine nucleotide metabolism may regulate the activity of the mitochondrial NAD(+)-dependent sirtuin (SIRT) deacetylases. We show that resveratrol increases the protein expression and half-life of P450 side chain cleavage enzyme (P450scc). The effects of resveratrol on P450scc protein levels and acetylation status are dependent on SIRT3 and SIRT5 expression. Stable overexpression of SIRT3 abrogates the cellular content of acetylated P450scc, concomitant with an increase in P450scc protein expression and cortisol secretion. Mutation of K148 and K149 to alanine stabilizes the expression of P450scc and results in a 1.5-fold increase in pregnenolone biosynthesis. Finally, resveratrol also increases the protein expression of P450 11β, another mitochondrial enzyme required for cortisol biosynthesis. Collectively, this study identifies a role for NAD(+)-dependent SIRT deacetylase activity in regulating the expression of mitochondrial steroidogenic P450.

  4. A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin.

    PubMed

    Slominski, Andrzej; Zjawiony, Jordan; Wortsman, Jacobo; Semak, Igor; Stewart, Jeremy; Pisarchik, Alexander; Sweatman, Trevor; Marcos, Josep; Dunbar, Chuck; C Tuckey, Robert

    2004-11-01

    Following up on our previous findings that the skin possesses steroidogenic activity from progesterone, we now show widespread cutaneous expression of the full cytochrome P450 side-chain cleavage (P450scc) system required for the intracellular catalytic production of pregnenolone, i.e. the genes and proteins for P450scc enzyme, adrenodoxin, adrenodoxin reductase and MLN64. Functionality of the system was confirmed in mitochondria from skin cells. Moreover, purified mammalian P450scc enzyme and, most importantly, mitochondria isolated from placenta and adrenals produced robust transformation of 7-dehydrocholesterol (7-DHC; precursor to cholesterol and vitamin D3) to 7-dehydropregnenolone (7-DHP). Product identity was confirmed by comparison with the chemically synthesized standard and chromatographic, MS and NMR analyses. Reaction kinetics for the conversion of 7-DHC into 7-DHP were similar to those for cholesterol conversion into pregnenolone. Thus, 7-DHC can form 7-DHP through P450scc side-chain cleavage, which may serve as a substrate for further conversions into hydroxy derivatives through existing steroidogenic enzymes. In the skin, 5,7-steroidal dienes (7-DHP and its hydroxy derivatives), whether synthesized locally or delivered by the circulation, may undergo UVB-induced intramolecular rearrangements to vitamin D3-like derivatives. This novel pathway has the potential to generate a variety of molecules depending on local steroidogenic activity and access to UVB.

  5. A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin

    PubMed Central

    Slominski, Andrzej; Zjawiony, Jordan; Wortsman, Jacobo; Semak, Igor; Stewart, Jeremy; Pisarchik, Alexander; Sweatman, Trevor; Marcos, Josep; Dunbar, Chuck; Tuckey, Robert C.

    2005-01-01

    Following up on our previous findings that the skin possesses steroidogenic activity from progesterone, we now show widespread cutaneous expression of the full cytochrome P450 side-chain cleavage (P450scc) system required for the intracellular catalytic production of pregnenolone, i.e. the genes and proteins for P450scc enzyme, adrenodoxin, adrenodoxin reductase and MLN64. Functionality of the system was confirmed in mitochondria from skin cells. Moreover, purified mammalian P450scc enzyme and, most importantly, mitochondria isolated from placenta and adrenals produced robust transformation of 7-dehydrocholesterol (7-DHC; precursor to cholesterol and vitamin D3) to 7-dehydropregnenolone (7-DHP). Product identity was confirmed by comparison with the chemically synthesized standard and chromatographic, MS and NMR analyses. Reaction kinetics for the conversion of 7-DHC into 7-DHP were similar to those for cholesterol conversion into pregnenolone. Thus, 7-DHC can form 7-DHP through P450scc side-chain cleavage, which may serve as a substrate for further conversions into hydroxy derivatives through existing steroidogenic enzymes. In the skin, 5,7-steroidal dienes (7-DHP and its hydroxy derivatives), whether synthesized locally or delivered by the circulation, may undergo UVB-induced intramolecular rearrangements to vitamin D3-like derivatives. This novel pathway has the potential to generate a variety of molecules depending on local steroidogenic activity and access to UVB. PMID:15511223

  6. Enzymatic metabolism of ergosterol by cytochrome p450scc to biologically active 17alpha,24-dihydroxyergosterol.

    PubMed

    Slominski, Andrzej; Semak, Igor; Zjawiony, Jordan; Wortsman, Jacobo; Gandy, Michael N; Li, Jinghu; Zbytek, Blazej; Li, Wei; Tuckey, Robert C

    2005-08-01

    We demonstrate the metabolism of ergosterol by cytochrome P450scc in either a reconstituted system or isolated adrenal mitochondria. The major reaction product was identified as 17alpha,24-dihydroxyergosterol. Purified P450scc also generated hydroxyergosterol as a minor product, which is probably an intermediate in the synthesis of 17alpha,24-dihydroxyergosterol. In contrast to cholesterol and 7-dehydrocholesterol, cleavage of the ergosterol side chain was not observed. NMR analysis clearly located one hydroxyl group to C24, with evidence that the second hydroxyl group is at C17. 17alpha,24-Dihydroxyergosterol inhibited cell proliferation of HaCaT keratinocytes and melanoma cells. Thus, in comparison with cholesterol and 7-dehydrocholesterol, the 24-methyl group and the C22-C23 double bond of ergosterol prevent side chain cleavage by P450scc and change the enzyme's hydroxylase activity from C22 and C20, to C24 and C17, generating bioactive product.

  7. The xenoestrogen, 4-nonylphenol, impaired steroidogenesis in previtellogenic oocyte culture of Atlantic cod (Gadus morhua) by targeting the StAR protein and P450scc expressions.

    PubMed

    Kortner, Trond M; Arukwe, Augustine

    2007-02-01

    The steroidogenic acute regulatory (StAR) protein and cytochrome P450-mediated cholesterol side-chain cleavage (P450scc) have been localized in most steroidogenic organs and are rapidly synthesized in response to acute tropic hormone stimulation. In this study, we present the development of cod previtellogenic oocyte in vitro culture system, histological and molecular methods for evaluating the effects of endocrine disruptors such as nonylphenol (NP) on steroid hormone levels, the StAR protein and P450scc. In addition, expression pattern of cyclin-B was studied, because of cyclin B's role as an indicator of oocyte growth in fish. The in vitro previtellogenic oocyte culture technique was based on an agarose floating method. Tissue was cultured in a humidified incubator at 10 degrees C for 4, 7, 14 and 21 d with different concentrations of nonylphenol (0 (control), 1, 10, 50 and 100 microM) dissolved in ethanol (0.3%). Gene expressions were detected using validated real-time polymerase chain reaction (PCR) with specific primers. Immunohistochemistry of the StAR protein and P450scc were performed using antisera prepared against synthetic peptide for both proteins. Estradiol-17beta (E2) and 11-ketotestosterone (11-KT) tissue levels were estimated using enzyme immunoassay. Our data show that nonylphenol produced a unique and consistent concentration-specific pattern of modulation for the StAR protein, P450scc and cyclin-B gene expression at day 14 after exposure. This pattern is generally described as increasing from 0 (control) to 1 and 10 microM, and decreased at 50 and 100 microM. The observed changes in the StAR protein, P450scc and cyclin-B levels showed a direct relationship with changes in 11-KT levels at day 14 after exposure. Cellular localization of StAR and P450scc were specific to the follicular cells of previtellogenic oocytes, but with no differences in staining intensities. No significant change in oocyte diameter was observed between the exposure groups

  8. Effects of fluoride and aluminum on expressions of StAR and P450scc of related steroidogenesis in guinea pigs' testis.

    PubMed

    Dong, Chunguang; Cao, Jinling; Cao, Chunfang; Han, Yichao; Wu, Shouyan; Wang, Shaolin; Wang, Jundong

    2016-03-01

    A lot of studies have shown that fluoride and aluminum have toxic effect on male reproductive system, but the mechanism of which and the interaction between fluoride and aluminum is still unknown. This study investigated the effects of fluoride (NaF) or/and aluminum (AlCl3) on serum testosterone level, gene and protein expression levels of Steroidogenic Acute Regulatory Protein (StAR) and Cytochrome P450 cholesterol side chain cleavage enzyme (P450scc) in the testes of guinea pigs. Fifty-two guinea pigs were divided randomly into four groups (Control, HiF, HiAl and HiF + HiAl). Fluoride (150 mg NaF/L) or/and aluminum (300 mg AlCl3/L) were orally administrated to male guinea pigs for 13 weeks. The results showed that F and Al reduced number and elevated abnormal ratio of sperm. Meanwhile, the concentrations of serum testosterone in all experimental groups were decreased. P450scc protein expression was significantly reduced in all treatment groups, and StAR expression was decreased remarkably in HiF group and HiF + HiAl group. The levels of StAR mRNA in three groups were reduced by 53.9%, 21.4% and 33.4%, respectively, while the expressions of P450scc mRNA were reduced by 67.8%, 17.0% and 47.8%. Therefore, we concluded that F induced the reduction in testosterone and sperm amount, and thus in lower fertility, which might occur as a consequence of depressed StAR and P450scc mRNA expression. There were no synergistic effects between F and Al, instead, Al weakened the toxicity of F to some extents. The results indicated that Al had antagonism effects on F.

  9. Ah Receptor-mediated impairment of interrenal steroidogenesis involves StAR protein and P450scc gene attenuation in rainbow trout.

    PubMed

    Aluru, Neelakanteswar; Renaud, Rick; Leatherland, John F; Vijayan, Mathilakath M

    2005-04-01

    The objective of the study was to investigate the impact of aryl hydrocarbon receptor (AhR) activation on interrenal steroidogenesis in rainbow trout. To this end, fish were fed AhR agonist (beta-naphthoflavone (BNF): 10 mg/kg body mass/day) and antagonist (alpha-naphthoflavone (ANF): 10 mg/kg body mass/day) either singly or in combination (ABNF) for 5 days to elucidate the mechanisms involved in AhR-mediated depression of cortisol production. Liver AhR protein expression was significantly elevated only with ABNF, but not with BNF and ANF compared to the control group. However, all three treatments (BNF, ANF, and ABNF) significantly elevated cytochrome P450 1A1 (CYP1A1) gene and protein expression in the kidney and liver, respectively. Also, these three treatment groups had significantly depressed ACTH-stimulated cortisol production in vitro compared to the control group. This attenuation of interrenal steroidogenesis corresponded with a lower mRNA abundance of steroidogenic acute regulatory (StAR) protein and cytochrome P450 cholesterol side chain cleavage enzyme (P450scc), but not 11beta-hydroxylase. Furthermore, in vitro incubation of head kidney pieces with 7-3H-pregnenolone failed to show any treatment effects on pathways downstream of P450scc, except for a significantly higher conversion to progesterone in the BNF and ANF groups. Plasma cortisol and glucose levels showed no significant change between the treated groups and control, but liver and brain glucocorticoid receptor (GR) protein expression was higher in the BNF group, and ANF abolished this response. Taken together, both BNF and ANF impaired cortisol production, and the mechanism may involve attenuation of StAR and P450scc, the rate limiting steps in steroidogenesis. Overall, endocrine disruption by xenobiotics acting via AhR includes impaired cortisol biosynthesis and abnormal cortisol target tissue GR responses in rainbow trout.

  10. An alternative pathway of vitamin D metabolism. Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2.

    PubMed

    Slominski, Andrzej; Semak, Igor; Wortsman, Jacobo; Zjawiony, Jordan; Li, Wei; Zbytek, Blazej; Tuckey, Robert C

    2006-07-01

    We report an alternative, hydroxylating pathway for the metabolism of vitamin D2 in a cytochrome P450 side chain cleavage (P450scc; CYP11A1) reconstituted system. NMR analyses identified solely 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2 derivatives. 20-Hydroxyvitamin D2 was produced at a rate of 0.34 mol x min(-1) x mol(-1) P450scc, and 17,20-dihydroxyvitamin D2 was produced at a rate of 0.13 mol x min(-1) x mol(-1). In adrenal mitochondria, vitamin D2 was metabolized to six monohydroxy products. Nevertheless, aminoglutethimide (a P450scc inhibitor) inhibited this adrenal metabolite formation. Initial testing of metabolites for biological activity showed that, similar to vitamin D2, 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2 inhibited DNA synthesis in human epidermal HaCaT keratinocytes, although to a greater degree. 17,20-Dihydroxyvitamin D2 stimulated transcriptional activity of the involucrin promoter, again to a significantly greater extent than vitamin D2, while the effect of 20-hydroxyvitamin D2 was statistically insignificant. Thus, P450scc can metabolize vitamin D2 to generate novel products, with intrinsic biological activity (at least in keratinocytes).

  11. An alternative pathway of vitamin D2 metabolism Cytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2

    PubMed Central

    Slominski, Andrzej; Semak, Igor; Wortsman, Jacobo; Zjawiony, Jordan; Li, Wei; Zbytek, Blazej; Tuckey, Robert C.

    2007-01-01

    We report an alternative, hydroxylating pathway for the metabolism of vitamin D2 in a cytochrome P450 side chain cleavage (P450scc; CYP11A1) reconstituted system. NMR analyses identified solely 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2 derivatives. 20-Hydroxyvitamin D2 was produced at a rate of 0.34 mol·min−1·mol−1 P450scc, and 17,20-dihydroxyvitamin D2 was produced at a rate of 0.13 mol·min−1·mol−1. In adrenal mitochondria, vitamin D2 was metabolized to six monohydroxy products. Nevertheless, aminoglutethimide (a P450scc inhibitor) inhibited this adrenal metabolite formation. Initial testing of metabolites for biological activity showed that, similar to vitamin D2, 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2 inhibited DNA synthesis in human epidermal HaCaT keratinocytes, although to a greater degree. 17,20-Dihydroxyvitamin D2 stimulated transcriptional activity of the involucrin promoter, again to a significantly greater extent than vitamin D2, while the effect of 20-hydroxyvitamin D2 was statistically insignificant. Thus, P450scc can metabolize vitamin D2 to generate novel products, with intrinsic biological activity (at least in keratinocytes). PMID:16817851

  12. Amino-steroids as inhibitors and probes of the active site of cytochrome P-450scc. Effects on the enzyme from different sources.

    PubMed

    Kellis, J T; Sheets, J J; Vickery, L E

    1984-02-01

    A series of analogues of cholesterol, each having a primary amine attached to a shortened side chain, were tested for their effects on cytochrome P-450scc from several different sources. Reconstituted enzyme systems using disrupted mitochondria from bovine adrenal and placenta, adult human adrenal and placenta, neonatal human adrenal, and rat adrenal and testis were used to assay for inhibitory effects on the side chain cleavage of cholesterol to pregnenolone. Two of the derivatives tested, 22-amino-23,24-bisnor-5-cholen-3 beta-ol and 23-amino-24-nor-5-cholen-3 beta-ol, were found to be potent inhibitors of this reaction; the derivatives in which the amine was attached closer to or further from the steroid ring, (20 R and S)-20-amino-5-pregnen-3 beta-ol and 24-amino-5-cholen-3 beta-ol, were much weaker inhibitors. In addition, spectral studies with rat adrenal mitochondria and a soluble preparation of human placental cytochrome P-450scc showed that binding of the 22-amine derivative to the enzyme produces difference spectra characteristic of nitrogen bonding to the heme; this indicates that the heme is positioned close to C-22 in the steroid-enzyme complex. These findings on the relative effectiveness of the amino-steroid inhibitors and the type of complex formed are similar to results obtained with purified bovine adrenocortical cytochrome P-450scc. This establishes that the proximity of the substrate binding site and the heme-iron catalytic site is a feature common to the enzyme from several sources and is therefore likely to be a necessary property of the active site structure.

  13. Alteration of brain and interrenal StAR protein, P450scc, and Cyp11beta mRNA levels in atlantic salmon after nominal waterborne exposure to the synthetic pharmaceutical estrogen ethynylestradiol.

    PubMed

    Lyssimachou, Angeliki; Arukwe, Augustine

    2007-04-01

    Pharmaceuticals are ubiquitous pollutants in the aquatic environment, where their potential effects on nontarget species like fish has only recently become subject of systematic investigations. Recently, it was shown that the documented xenoestrogen nonylphenol produced variations in brain steroidogenic acute regulatory (StAR) protein, cytochrome P-450-mediated cholesterol side-chain cleavage (P450scc), and cytochrome P-45011beta hydroxylase (CYP11beta) gene transcripts of exposed juvenile salmon (Arukwe, 2005). In the present study, experiments were undertaken to examine the effect of the synthetic pharmaceutical endocrine disruptor ethynylestradiol (EE2), given in water at 5 or 50 ng/L and sampled at d 0 (control), 3, and 7 after exposure, on these key and rate-limiting brain and interrenal steroidogenic pathways of juvenile salmon using quantitative (real-time) polymerase chain reaction (qPCR). Our data, which are based on nominal exposure concentrations, show that brain and head kidney StAR and P450scc expression were modulated by EE2 in a time- and concentration-specific manner. While the StAR protein and P450scc showed EE2 concentration-dependent transcriptional increases in the brain and head kidney at d 3 after exposure, no significant effect was observed at d 7. The EE2 induced effects at d 7 were underscored because the carrier solvent (dimethyl sulfoxide, DMSO) produced significant induction of the StAR protein and P450scc in both the brain and head kidney at d 7 compared to d 3 postexposure. CYP11beta transcript was detected in the brain and head kidney, where the expression patterns were modulated by EE2 in a concentration-and time-specific manner. In the brain, DMSO produced significant changes in the CYP11beta gene expression at d 7 compared to d 3 after exposure. These changes in the levels of StAR, P450scc, and CYP11beta mRNA levels in important steroidogenic organs suggest that the experimental animals are experiencing a time-dependent impaired

  14. Cytochrome P450 side-chain cleavage (P450scc) in the hen ovary. I. Regulation of P450scc messenger RNA levels and steroidogenesis in theca cells of developing follicles.

    PubMed

    Kowalski, K I; Tilly, J L; Johnson, A L

    1991-12-01

    We have recently shown that granulosa cells from hen ovarian follicles, collected at a stage of development 2-3 wk prior to ovulation (e.g. 6-8 mm in diameter) are steroidogenically inactive. Therefore, the hypothesis tested in the present studies was that theca cells from follicles at this stage of development must contain sufficient levels of functional cytochrome P450 side-chain cleavage (P450scc) enzyme to produce the progestin precursor required for the synthesis of androgens and estrogens. Northern blot analysis of total theca RNA collected from 6-8-mm follicles indicated the presence of a single P450scc mRNA transcript of approximately 2 kb whose expression was increased following an 8-h preincubation with 200 ng/ml ovine LH (oLH) or 10 microM forskolin. Western blot analysis of crude mitochondrial protein revealed a band of immunoreactive P450scc protein of approximately 53 kDa that was determined to be capable of converting 25-hydroxycholesterol to pregnenolone in a cell-free system. In the second set of studies, conducted to examine the cellular regulation of steroidogenesis in isolated theca cells of 6-8-mm follicles, theca cells were found to produce measurable basal levels of cAMP, progesterone, androstenedione, and estradiol following a 3-h incubation of 5 x 10(5) cells. Furthermore, significant dose-dependent increases in steroidogenesis were observed in response to oLH (0.2-200 ng/ml), chicken FSH (cFSH; 20-200 ng/ml), cholera toxin (0.002-20 ng/ml), and 8-bromo-cAMP (0.1-3.33 mM). Phorbol 12-myristate 13-acetate (PMA; 10-167 nM) also stimulated dose-dependent increases in basal progesterone, androstenedione, and estradiol production. In addition, while PMA had no effect on oLH (200 ng/ml)-promoted cAMP accumulation, or on oLH (20 ng/ml)- or 8-bromo-cAMP (1 mM)-stimulated progesterone production, it attenuated oLH-induced and 8-bromo-cAMP-induced androstenedione and estradiol accumulation. We conclude that theca cells from 6-8-mm follicles possess m

  15. 20-Hydroxyvitamin D3, a Product of Vitamin D3 Hydroxylation by Cytochrome P450scc, Stimulates Keratinocyte Differentiation

    PubMed Central

    Zbytek, Blazej; Janjetovic, Zorica; Tuckey, Robert C.; Zmijewski, Michal A.; Sweatman, Trevor W.; Jones, Emily; Nguyen, Minh N.; Slominski, Andrzej T.

    2008-01-01

    It has been shown that mammalian cytochrome P450scc can metabolize vitamin D3 to 20-hydroxyvitamin D3 (20(OH)D3) and 20,22(OH)2D3. To define the biological significance of this pathway, we tested the effects of 20(OH)D3 on the differentiation program of keratinocytes and on the expression of enzymes engaged in vitamin D3 metabolism. Immortalized HaCaT and adult human epidermal keratinocytes were used as a model and the effects of 20(OH)D3 were compared with those of 25(OH)D3 and 1,25(OH)2D3. 20(OH)D3 inhibited proliferation and caused G2/M arrest. 20(OH)D3 stimulated involucrin and inhibited cytokeratin 14 expression. The potency of 20(OH)D3 was comparable to that of 1,25(OH)2D3. 20(OH)D3 decreased the expression of cytochrome P450 enzyme (CYP)27A1 and CYP27B1, however, having only slight effect on CYP24. The effect of 20(OH)D3 was dependent on the vitamin D receptor (VDR). As shown by electrophoretic mobility shift assay, 20(OH)D3 stimulated the binding of nuclear proteins to the VDRE. Transfection of cells with VDR-specific siRNA decreased 20(OH)D3-stimulated transcriptional activity of the VDRE promoter and the expression of involucrin and CYP24 mRNA. Therefore, the above studies identify 20(OH)D3 as a biologically active secosteroid that induces keratinocyte differentiation. These data imply that the previously unreported pathway of vitamin D3 metabolism by P450scc may have wider biological implications depending, for example, on the extent of adrenal gland or cutaneous metabolism. PMID:18368131

  16. Steroidogenic acute regulatory (StAR) protein and cholesterol side-chain cleavage (P450scc) as molecular and cellular targets for 17alpha-ethynylestradiol in salmon previtellogenic oocytes.

    PubMed

    Vang, Siv-Hege; Kortner, Trond M; Arukwe, Augustine

    2007-12-01

    Gonadal steroids are known to modulate both the synthesis and the release of gonadotropins by the pituitary and influence several brain functions that are apparently responsible for gender-specific differences in the regulation of the hypothalamus-pituitary-gonadal (HPG) axis. It is believed that the true rate-limiting step in acute steroid production is the movement of cholesterol across the mitochondrial membrane by the steroidogenic acute regulatory (StAR) protein and subsequent conversion to pregnenolone by P450-mediated cholesterol side chain cleavage (P450 scc). In the present study, we have evaluated the effects of 17alpha-ethynylestradiol (EE2) on salmon previtellogenic oocytes using an in vitro culture system and molecular, histological, and physiological methods. The in vitro culture technique was based on an agarose floating method recently validated for xenoestrogens in our laboratory. Tissue was cultured in a humidified incubator at 10 degrees C for 3, 7, and 14 days with different concentrations of EE2 [0 (control), 0.01, 0.1, and 1 microM] dissolved in ethanol (0.1%). The StAR, P450 scc, P450 arom isoforms, and insulin-like growth factor 2 (IGF-2) mRNA expressions were performed using validated real-time polymerase chain reaction (PCR) with specific primers, and immunohistochemistry of the StAR and P450 scc proteins was performed using antisera prepared against synthetic peptide for both proteins and estradiol-17beta (E2); testosterone (T) and 11-ketotestosterone (11-KT) tissue levels were performed using enzyme immunoassay (EIA). Our data show that EE2 produced time- and concentration-specific effects on the StAR protein, P450 scc, P450 arom isoforms, and IGF-2 gene expressions in salmon gonadal tissues. Cellular expression of the StAR and P450 scc proteins was mainly demonstrated in follicular cells of the oocyte membrane, showing time- and EE2 concentration-dependent differences in staining intensities. Tissue levels of E2, T, and 11-KT in salmon

  17. In vivo evidence for a novel pathway of vitamin D3 metabolism initiated by P450scc and modified by CYP27B1

    PubMed Central

    Slominski, Andrzej T.; Kim, Tae-Kang; Shehabi, Haleem Z.; Semak, Igor; Tang, Edith K. Y.; Nguyen, Minh N.; Benson, Heather A. E.; Korik, Elena; Janjetovic, Zorica; Chen, Jianjun; Yates, Charles R.; Postlethwaite, Arnold; Li, Wei; Tuckey, Robert C.

    2012-01-01

    We define previously unrecognized in vivo pathways of vitamin D3 (D3) metabolism generating novel D3-hydroxyderivatives different from 25-hydroxyvitamin D3 [25(OH)D3] and 1,25(OH)2D3. Their novel products include 20-hydroxyvitamin D3 [20(OH)D3], 22(OH)D3, 20,23(OH)2D3, 20,22(OH)2D3, 1,20(OH)2D3, 1,20,23(OH)3D3, and 17,20,23(OH)3D3 and were produced by placenta, adrenal glands, and epidermal keratinocytes. We detected the predominant metabolite [20(OH)D3] in human serum with a relative concentration ∼20 times lower than 25(OH)D3. Use of inhibitors and studies performed with isolated mitochondria and purified enzymes demonstrated involvement of the steroidogenic enzyme cytochrome P450scc (CYP11A1) as well as CYP27B1 (1α-hydroxylase). In placenta and adrenal glands with high CYP11A1 expression, the predominant pathway was D3 → 20(OH)D3 → 20,23(OH)2D3 → 17,20,23(OH)3D3 with further 1α-hydroxylation, and minor pathways were D3 → 25(OH)D3 → 1,25(OH)2D3 and D3 → 22(OH)D3 → 20,22(OH)2D3. In epidermal keratinocytes, we observed higher proportions of 22(OH)D3 and 20,22(OH)2D3. We also detected endogenous production of 20(OH)D3, 22(OH) D3, 20,23(OH)2D3, 20,22(OH)2D3, and 17,20,23(OH)3D3 by immortalized human keratinocytes. Thus, we provide in vivo evidence for novel pathways of D3 metabolism initiated by CYP11A1, with the product profile showing organ/cell type specificity and being modified by CYP27B1 activity. These findings define the pathway intermediates as natural products/endogenous bioregulators and break the current dogma that vitamin D is solely activated through the sequence D3 → 25(OH)D3 → 1,25(OH)2D3.—Slominski, A. T., Kim, T.-K., Shehabi, H. Z., Semak, I., Tang, E. K. Y., Nguyen, M. N., Benson, H. A. E., Korik, E., Janjetovic, Z., Chen, J., Yates, C. R., Postlethwaite, A., Li, W., Tuckey, R. C. In vivo evidence for a novel pathway of vitamin D3 metabolism initiated by P450scc and modified by CYP27B1. PMID:22683847

  18. Modulation of brain steroidogenesis by affecting transcriptional changes of steroidogenic acute regulatory (StAR) protein and cholesterol side chain cleavage (P450scc) in juvenile Atlantic salmon (Salmo salar) is a novel aspect of nonylphenol toxicity.

    PubMed

    Arukwe, Augustine

    2005-12-15

    Gene expression patterns for key brain steroidogenic (StAR, P450scc, CYP11beta) and xenobiotic- and steroid-metabolizing enzymes (CYP1A1 and CYP3A) have been investigated in waterborne nonylphenol (5, 15, and 50 microg/ L) treated juvenile Atlantic salmon (Salmo salar), in addition to carrier vehicle (ethanol) exposed fish, sampled at different time intervals (0, 3, and 7 days) after exposure. Gene expression patterns were studied using the quantitative polymerase chain reaction (real-time PCR). Treatment of juvenile salmon with nonylphenol caused significant induction of steroidogenic acute regulatory (StAR) protein mRNA at day 7 postexposure in the group receiving 15 microg of nonylphenol/L. P450scc was first induced in the group treated with 5 microg of nonylphenol/L at day 7; thereafter, an apparent nonylphenol-concentration-dependent decrease in P450scc mRNA was observed. CYP11beta mRNA was significantly induced at day 3 after exposure to 5 betag of nonylphenol/L; thereafter, CYP11beta mRNA levels were inhibited below control levels in the 15 and 50 microg of nonylphenol/L groups at day 3. At day 7, significant induction of CYP11beta mRNA was observed only in the group exposed to 15 microg of nonylphenol/L. For CYP1A1 mRNA, apparent nonylphenol-concentration-dependent decreases were observed at day 7 postexposure. CYP3A mRNA was significantly induced by all nonylphenol exposure concentrations at day 7. When exposed groups were compared, CYP3A transcript was significantly induced between 5 and 15 microg of nonylphenol/ L, and decreased between 15 and 50 microg of nonylphenol/ L. The ethanol control showed a significant reduction of CYP3A mRNA at day 3 postexposure. The present study has demonstrated variations in three key steroidogenic proteins and xenobiotic- and steroid-metabolizing CYP isoenzyme gene transcripts in the brain of nonylphenol-exposed juvenile salmon. Therefore, the present study represents a novel aspect of neuroendocrine effects of

  19. In vivo evidence for a novel pathway of vitamin D₃ metabolism initiated by P450scc and modified by CYP27B1.

    PubMed

    Slominski, Andrzej T; Kim, Tae-Kang; Shehabi, Haleem Z; Semak, Igor; Tang, Edith K Y; Nguyen, Minh N; Benson, Heather A E; Korik, Elena; Janjetovic, Zorica; Chen, Jianjun; Yates, Charles R; Postlethwaite, Arnold; Li, Wei; Tuckey, Robert C

    2012-09-01

    We define previously unrecognized in vivo pathways of vitamin D(3) (D3) metabolism generating novel D3-hydroxyderivatives different from 25-hydroxyvitamin D(3) [25(OH)D3] and 1,25(OH)(2)D3. Their novel products include 20-hydroxyvitamin D(3) [20(OH)D3], 22(OH)D3, 20,23(OH)(2)D3, 20,22(OH)(2)D3, 1,20(OH)(2)D3, 1,20,23(OH)(3)D3, and 17,20,23(OH)(3)D3 and were produced by placenta, adrenal glands, and epidermal keratinocytes. We detected the predominant metabolite [20(OH)D3] in human serum with a relative concentration ∼20 times lower than 25(OH)D3. Use of inhibitors and studies performed with isolated mitochondria and purified enzymes demonstrated involvement of the steroidogenic enzyme cytochrome P450scc (CYP11A1) as well as CYP27B1 (1α-hydroxylase). In placenta and adrenal glands with high CYP11A1 expression, the predominant pathway was D3 → 20(OH)D3 → 20,23(OH)(2)D3 → 17,20,23(OH)(3)D3 with further 1α-hydroxylation, and minor pathways were D3 → 25(OH)D3 → 1,25(OH)(2)D3 and D3 → 22(OH)D3 → 20,22(OH)(2)D3. In epidermal keratinocytes, we observed higher proportions of 22(OH)D3 and 20,22(OH)(2)D3. We also detected endogenous production of 20(OH)D3, 22(OH) D3, 20,23(OH)(2)D3, 20,22(OH)(2)D3, and 17,20,23(OH)(3)D3 by immortalized human keratinocytes. Thus, we provide in vivo evidence for novel pathways of D3 metabolism initiated by CYP11A1, with the product profile showing organ/cell type specificity and being modified by CYP27B1 activity. These findings define the pathway intermediates as natural products/endogenous bioregulators and break the current dogma that vitamin D is solely activated through the sequence D3 → 25(OH)D3 → 1,25(OH)(2)D3.

  20. Active site-directed inhibitors of cytochrome P-450scc. Structural and mechanistic implications of a side chain-substituted series of amino-steroids.

    PubMed

    Sheets, J J; Vickery, L E

    1983-10-10

    A series of analogues of cholesterol, each having a shortened side chain and a primary amine group, were prepared and tested for their effects on bovine adrenocortical cholesterol side chain cleavage cytochrome P-450 (P-450scc). A previous study had shown that one derivative, 22-amino-23,24-bisnor-5-cholen-3 beta-ol, is a potent competitive inhibitor of the enzyme and forms a complex in which the steroid ring binds to the cholesterol site and the side chain amine forms a bond with the heme iron (Sheets, J. J., and Vickery, L. E. (1982) Proc. Natl. Acad. Sci. U.S.A. 79, 5773-5777). In the studies reported here, the 23-amine derivative, 23-amino-24-nor-5-cholen-3 beta-ol, was found to be an equally potent inhibitor and to be competitive with respect to cholesterol (Ki = 38 nM). Binding of the 23-amine to P-450scc also caused formation of a low spin complex with an absorption maximum at 422 nm, indicative of a nitrogen-donor ligand. Other derivatives in which the side chain amine was linked closer to the steroid, 17 beta-amino-5-androsten-3 beta-ol and (20 R + S)-20-amino-5-pregnen-3 beta-ol, were found to be only very weak inhibitors (I50 greater than 100 microM) and did not produce the 422 nm spectral form when bound. Derivatives in which the amine was attached a greater distance from the steroid ring, 24-amino-5-cholen-3 beta-ol and 25-amino-26,27-bisnor-5-cholesten-3 beta-ol, caused a progressive decrease in inhibitory potency and a failure to produce the 422 nm form on binding. The dependence of the type of interaction of these amino-steroids with P-450scc upon the amine position establishes that the steroid binding site and the heme catalytic site of the enzyme are fixed within a specific distance of one another. The heme appears to be located sufficiently close to the position that the side chain of cholesterol would occupy to allow for direct attack of an iron-bound oxidant to occur during hydroxylation and side chain cleavage.

  1. Steroidogenic acute regulatory (StAR) protein and cholesterol side-chain cleavage (P450scc)-regulated steroidogenesis as an organ-specific molecular and cellular target for endocrine disrupting chemicals in fish.

    PubMed

    Arukwe, Augustine

    2008-12-01

    Biologically active steroids are synthesised de novo in specialised cells of several organs, including the adrenal gland, testis, ovary, brain, placenta and adipose tissue. Regardless of organ or tissue, the rate-limiting step in steroid hormone synthesis is the movement of cholesterol across the mitochondrial membrane (i.e. from the outer to the inner membrane) mediated by the steroidogenic acute regulatory (StAR) protein. Subsequent conversion of cholesterol to pregnenolone by cytochrome P450 side-chain cleavage (P450scc) represents the initiation of steroidogenesis. Chemically mediated disruption of StAR and P450scc expression may represent the first step in the sequence of related event cascades underlying xenoestrogen-induced toxicity and transmittable disturbances to the whole organism level. This may include, but is not limited to, alterations in sexual differentiation, growth, reproduction, development and metabolism. Despite the integral role of StAR and P450scc in acute steroidogenesis, and popular demand from regulatory agencies, bioassays for evaluating the effect of endocrine-disrupting chemicals have the potential to overlook chemicals that may modulate estrogenic responses through mechanisms that do not involve direct binding to estrogen receptors (ERs). In addition to their effect as direct ER agonists, the effects of endocrine disruptors may be evaluated and interpreted as interference with steroidogenesis and with the steroidal regulation of the normal development and function of juvenile, male and female individuals. Knowledge of these effects is scarce, indicating that relatively little is known about the mechanisms or mode-of-action of chemical alterations to steroidogenesis and their potential toxicity for wildlife species. In addition, analytical methods for the complete adaptation of these responses as biomarkers of response and effect are yet to be properly validated.

  2. Dasatinib-loaded albumin nanoparticles possess diminished endothelial cell barrier disruption and retain potent anti-leukemia cell activity

    PubMed Central

    Li, Zhenyu; Shetty, Sreerama; Fu, Jian

    2016-01-01

    Dasatinib (DAS), a second-generation tyrosine kinase inhibitor, is highly effective in treating chronic myeloid leukemia and Philadelphia chromosome-positive acute lymphoblastic leukemia. However, its clinical use is limited due to serious adverse effects. DAS can disrupt endothelial barrier integrity and increase endothelial permeability which may cause peripheral edema and pleural effusion. Albumin nanoparticles (NPs) as a drug carrier may serve as a useful tool for cell-selective drug delivery to reduce DAS-induced endothelial hyperpermeability and maintain endothelial barrier integrity. In this study, we reported that DAS-loaded NPs exhibited potent anti-leukemia efficacy as DAS alone. Importantly, albumin NPs as a drug carrier markedly reduced DAS-induced endothelial hyperpermeability by restraining the inhibition of Lyn kinase signaling pathway in endothelial cells. Therefore, albumin NPs could be a potential tool to improve anti-leukemia efficacy of DAS through its cell-selective effects. PMID:27391073

  3. Myricetin is a novel inhibitor of human inosine 5'-monophosphate dehydrogenase with anti-leukemia activity.

    PubMed

    Pan, Huiling; Hu, Qian; Wang, Jingyuan; Liu, Zehui; Wu, Dang; Lu, Weiqiang; Huang, Jin

    2016-09-02

    Human inosine 5'-monophosphate dehydrogenase (hIMPDH) is a rate-limiting enzyme in the de novo biosynthetic pathway of purine nucleotides, playing crucial roles in cellular proliferation, differentiation, and transformation. Dysregulation of hIMPDH expression and activity have been found in a variety of human cancers including leukemia. In this study, we found that myricetin, a naturally occurring phytochemical existed in berries, wine and tea, was a novel inhibitor of human type 1 and type 2 IMPDH (hIMPDH1/2) with IC50 values of 6.98 ± 0.22 μM and 4.10 ± 0.14 μM, respectively. Enzyme kinetic analysis using Lineweaver-Burk plot revealed that myricetin is a mix-type inhibitor for hIMPDH1/2. Differential scanning fluorimetry and molecular docking simulation data demonstrate that myricetin is capable of binding with hIMPDH1/2. Myricetin treatment exerts potent anti-proliferative and pro-apoptotic effects on K562 human leukemia cells in a dose-dependent manner. Importantly, cytotoxicity of myricetin on K562 cells were markedly attenuated by exogenous addition of guanosine, a salvage pathway of maintaining intracellular pool of guanine nucleotides. Taking together, these results indicate that natural product myricetin exhibits potent anti-leukemia activity by interfering with purine nucleotides biosynthetic pathway through the suppression of hIMPDH1/2 catalytic activity.

  4. In Vivo Anti-Leukemia, Quantum Chemical Calculations and ADMET Investigations of Some Quaternary and Isothiouronium Surfactants

    PubMed Central

    El-Henawy, Ahmed A.; Khowdiary, Manal M.; Badawi, Abdelfattah B.; Soliman, Hussein M.

    2013-01-01

    Anti-leukemia screening of previously prepared isothiouronium and quaternary salts was performed, and some salts exhibited promising activity as anticancer agents. Quantum chemical calculations were utilized to explore the electronic structure and stability of these compounds. Computational studies have been carried out at the PM3 semiempirical molecular orbitals level, to establish the HOMO-LUMO, IP and ESP mapping of these compounds. The ADMET properties were also studied to gain a clear view of the potential oral bioavailability of these compounds. The surface properties calculated included critical micelle concentration (CMC), maximum surface excess (Γmax), minimum surface area (Amin), free energy of micellization (ΔGomic) and adsorption (ΔGoads). PMID:24276171

  5. Enhancing in vivo Bioavailability in Beagle Dogs of GLM-7 as a Novel Anti-Leukemia Drug through a Self-Emulsifying Drug Delivery System for Oral Delivery.

    PubMed

    Wang, Yuli; Yu, Ning; Guo, Rui; Yang, Meiyan; Shan, Li; Huang, Wei; Gong, Wei; Shao, Shuai; Chen, Xiaoping; Gao, Chunsheng

    2016-01-01

    GLM-7 is a novel anti-leukemia drug in the pre-clinical study. The previous study shows that GLM-7 is a poorly water-soluble drug with low oral bioavailability. In this study, we employed the self-emulsifying drug delivery system (SEDDS) to improve the oral bioavailability of GLM-7. The GLM-7 SEDDS formulation was prepared using MCT as oil, ovolecithin as surfactant and Transcutol as co-surfactant, and the formulation parameters were optimized by the response surface methodology. The optimized GLM-7 SEDDS formulation showed a stable liquid state, and can automatically emulsify to form the isotropic emulsion once exposure to the water phase. The generated emulsion showed the spherical shape, and had an average size of about 399 nm and a zeta potential of about -42 mV. Compared to the GLM-7 dissolution less than 1.4% from pure GLM-7 powder (reference), the GLM-7 SEDDS formulation could remarkably enhance the in vitro dissolution to 83% in the medium of 0.1N HCL. The in vivo oral bioavailability of GLM-7 SEDDS formulation was investigated in beagle dogs. The results demonstrated that the GLM-7 SEDDS formulation significantly enhanced the plasma concentrations of GLM-7, and the Cmax reached to 878 ng/ml and was 9.2 folds as high as the Cmax 95.85 ng/ml of reference. Moreover, the area under the curve (AUC) of GLM-7 SEDDS formulation was 13.6 times higher than that of reference, which suggested that the SEDDS formulation remarkably increased the oral bioavailability of GLM-7.

  6. Overexpression of the steroidogenic enzyme cytochrome P450 side chain cleavage in the ventral tegmental area increases 3α,5α-THP and reduces long-term operant ethanol self-administration.

    PubMed

    Cook, Jason B; Werner, David F; Maldonado-Devincci, Antoniette M; Leonard, Maggie N; Fisher, Kristen R; O'Buckley, Todd K; Porcu, Patrizia; McCown, Thomas J; Besheer, Joyce; Hodge, Clyde W; Morrow, A Leslie

    2014-04-23

    Neuroactive steroids are endogenous neuromodulators capable of altering neuronal activity and behavior. In rodents, systemic administration of endogenous or synthetic neuroactive steroids reduces ethanol self-administration. We hypothesized this effect arises from actions within mesolimbic brain regions that we targeted by viral gene delivery. Cytochrome P450 side chain cleavage (P450scc) converts cholesterol to pregnenolone, the rate-limiting enzymatic reaction in neurosteroidogenesis. Therefore, we constructed a recombinant adeno-associated serotype 2 viral vector (rAAV2), which drives P450scc expression and neuroactive steroid synthesis. The P450scc-expressing vector (rAAV2-P450scc) or control GFP-expressing vector (rAAV2-GFP) were injected bilaterally into the ventral tegmental area (VTA) or nucleus accumbens (NAc) of alcohol preferring (P) rats trained to self-administer ethanol. P450scc overexpression in the VTA significantly reduced ethanol self-administration by 20% over the 3 week test period. P450scc overexpression in the NAc, however, did not alter ethanol self-administration. Locomotor activity was unaltered by vector administration to either region. P450scc overexpression produced a 36% increase in (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP, allopregnanolone)-positive cells in the VTA, but did not increase 3α,5α-THP immunoreactivity in NAc. These results suggest that P450scc overexpression and the resultant increase of 3α,5α-THP-positive cells in the VTA reduces ethanol reinforcement. 3α,5α-THP is localized to neurons in the VTA, including tyrosine hydroxylase neurons, but not astrocytes. Overall, the results demonstrate that using gene delivery to modulate neuroactive steroids shows promise for examining the neuronal mechanisms of moderate ethanol drinking, which could be extended to other behavioral paradigms and neuropsychiatric pathology.

  7. Attenuated A20 expression of acute myeloid leukemia-derived dendritic cells increased the anti-leukemia immune response of autologous cytolytic T cells.

    PubMed

    Zhang, Xiaoying; Su, Yongfeng; Song, Haifeng; Yu, Zhiyong; Zhang, Bin; Chen, Hu

    2014-06-01

    Previous studies reported leukemic cells from acute myeloid leukemia (AML) patients can differentiate into dendritic cells (DCs), which had some immunoregulatory dysfunctions to effectively stimulate autologous CTLs' anti-leukemia immune response. The zinc-finger protein A20, a negative regulator of the nuclear factor (NF)-κB pathway, was found to play a crucial role in controlling the maturation and function of human monocyte-derived DCs. However, the effects of A20 in AML derived DCs (AML-DCs) have not yet been evaluated. In this study, A20 expression was up-regulated in AML-DCs activated with tumor necrosis factor (TNF)-α. Then, A20 attenuation with siRNA in AML-DC enhanced the expression of several co-stimulatory molecules and proinflammatory cytokines. Furthermore, after A20 attenuation in AML-DCs, the autologous cytolytic T cells (CTLs) induced by AML-DCs had higher killing capability and specificity for primary AML cells. Additionally, receptor-interacting protein (RIP) and the NF-κBp65 pathway were elevated in AML-DCs when A20 was reduced. Hence, this study identified A20 as a negative regulator for controlling AML-DC maturation and immunostimulatory potency, as A20 down-regulation resulted in AML-DCs with enhanced autologous CTLs immune capacity through the NF-κB pathway.

  8. The plant alkaloid and anti-leukemia drug homoharringtonine sensitizes resistant human colorectal carcinoma cells to TRAIL-induced apoptosis via multiple mechanisms.

    PubMed

    Beranova, Lenka; Pombinho, Antonio R; Spegarova, Jarmila; Koc, Michal; Klanova, Magdalena; Molinsky, Jan; Klener, Pavel; Bartunek, Petr; Andera, Ladislav

    2013-06-01

    TNF-related apoptosis-inducing ligand (TRAIL) is a pro-apoptotic ligand from the TNF-alpha family that is under consideration, along with agonistic anti-TRAIL receptor antibodies, as a potential anti-tumor agent. However, most primary human tumors are resistant to monotherapy with TRAIL apoptogens, and thus the potential applicability of TRAIL in anti-tumor therapy ultimately depends on its rational combination with drugs targeting these resistances. In our high-throughput screening for novel agents/drugs that could sensitize TRAIL-resistant colorectal cancer cells to TRAIL-induced apoptosis, we found homoharringtonine (HHT), a cephalotaxus alkaloid and tested anti-leukemia drug, to be a very effective, low nanomolar enhancer of TRAIL-mediated apoptosis/growth suppression of these resistant cells. Co-treatment of TRAIL-resistant RKO or HT-29 cells with HHT and TRAIL led to the effective induction of apoptosis and the complete elimination of the treated cells. HHT suppressed the expression of the anti-apoptotic proteins Mcl-1 and cFLIP and enhanced the TRAIL-triggered activation of JNK and p38 kinases. The shRNA-mediated down-regulation of cFLIP or Mcl-1 in HT-29 or RKO cells variably enhanced their TRAIL-induced apoptosis but it did not markedly sensitize them to TRAIL-mediated growth suppression. However, with the notable exception of RKO/sh cFLIP cells, the downregulation of cFLIP or Mcl-1 significantly lowered the effective concentration of HHT in HHT + TRAIL co-treatment. Combined HHT + TRAIL therapy also led to the strong suppression of HT-29 tumors implanted into immunodeficient mice. Thus, HHT represents a very efficient enhancer of TRAIL-induced apoptosis with potential application in TRAIL-based, anti-cancer combination therapy.

  9. Multimodal effects of small molecule ROCK and LIMK inhibitors on mitosis, and their implication as anti-leukemia agents.

    PubMed

    Oku, Yusuke; Tareyanagi, Chiaki; Takaya, Shinichi; Osaka, Sayaka; Ujiie, Haruki; Yoshida, Kentaro; Nishiya, Naoyuki; Uehara, Yoshimasa

    2014-01-01

    Accurate chromosome segregation is vital for cell viability. Many cancer cells show chromosome instability (CIN) due to aberrant expression of the genes involved in chromosome segregation. The induction of massive chromosome segregation errors in such cancer cells by small molecule inhibitors is an emerging strategy to kill these cells selectively. Here we screened and characterized small molecule inhibitors which cause mitotic chromosome segregation errors to target cancer cell growth. We screened about 300 chemicals with known targets, and found that Rho-associated coiled-coil kinase (ROCK) inhibitors bypassed the spindle assembly checkpoint (SAC), which delays anaphase onset until proper kinetochore-microtubule interactions are established. We investigated how ROCK inhibitors affect chromosome segregation, and found that they induced microtubule-dependent centrosome fragmentation. Knockdown of ROCK1 and ROCK2 revealed their additive roles in centrosome integrity. Pharmacological inhibition of LIMK also induced centrosome fragmentation similar to that by ROCK inhibitors. Inhibition of ROCK or LIMK hyper-stabilized mitotic spindles and impaired Aurora-A activation. These results suggested that ROCK and LIMK are directly or indirectly involved in microtubule dynamics and activation of Aurora-A. Furthermore, inhibition of ROCK or LIMK suppressed T cell leukemia growth in vitro, but not peripheral blood mononuclear cells. They induced centrosome fragmentation and apoptosis in T cell leukemia cells. These results suggested that ROCK and LIMK can be a potential target for anti-cancer drugs.

  10. Immunohistochemical evidence: testicular and scented glandular androgen synthesis in muskrats (Ondatra zibethicus) during the breeding season.

    PubMed

    Li, Q; Weng, J; Zhang, H; Lu, L; Ma, X; Wang, Q; Cao, H; Liu, S; Xu, M; Weng, Q; Watanabe, G; Taya, K

    2011-10-19

    In order to elucidate the relationship between androgens and the function of the muskrat (Ondatra zibethicus) scented glands during the breeding season, we investigated immunolocalization of steroidogenic enzymes P450scc, 3βHSD and P450c17 in the muskrat testes and scented glands. Nine adult muskrats were obtained in March (n=3), May (n=3) and July (n=3) 2010. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal P450scc, human placental 3βHSD and porcine testicular P450c17. Histologically, all types of spermatogenic cells including mature-phase spermatozoa in seminiferous tubules were observed in all testes. Glandular cells, interstitial cells, epithelial cells and excretory tubules were identified in scented glands during the breeding season. P450scc, 3βHSD and P450c17 were only identified in Leydig cells during the breeding season; P450scc and P450c17 were observed in glandular cells of scented glands, however, 3βHSD was not found in scented glands during the breeding season. These novel findings provide the first evidence showing that scented glands of the muskrats are capable of locally synthesizing androgens and androgens acting via an endocrine, autocrine or paracrine manner may play an important role in scented gland function during the breeding season.

  11. Homozygous disruption of P450 side-chain cleavage (CYP11A1) is associated with prematurity, complete 46,XY sex reversal, and severe adrenal failure.

    PubMed

    Hiort, Olaf; Holterhus, Paul-Martin; Werner, Ralf; Marschke, Christine; Hoppe, Ute; Partsch, Carl-Joachim; Riepe, Felix G; Achermann, John C; Struve, Dagmar

    2005-01-01

    Disruption of the P450 side-chain cleavage cytochrome (P450scc) enzyme due to deleterious mutations of the CYP11A1 gene is thought to be incompatible with fetal survival because of impaired progesterone production by the fetoplacental unit. We present a 46,XY patient with a homozygous disruption of CYP11A1. The child was born prematurely with complete sex reversal and severe adrenal insufficiency. Laboratory data showed diminished or absent steroidogenesis in all pathways. Molecular genetic analysis of the CYP11A1 gene revealed a homozygous single nucleotide deletion leading to a premature termination at codon position 288. This mutation will delete highly conserved regions of the P450scc enzyme and thus is predicted to lead to a nonfunctional protein. Both healthy parents were heterozygous for this mutation. Our report demonstrates that severe disruption of P450scc can be compatible with survival in rare instances. Furthermore, defects in this enzyme are inherited in an autosomal-recessive fashion, and heterozygote carriers can be healthy and fertile. The possibility of P450scc-independent pathways of steroid synthesis in addition to the current concept of luteoplacental shift of progesterone synthesis in humans has to be questioned.

  12. Autoantibodies against Cytochrome P450 Side-Chain Cleavage Enzyme in Dogs (Canis lupus familiaris) Affected with Hypoadrenocorticism (Addison’s Disease)

    PubMed Central

    Boag, Alisdair M.; Christie, Michael R.; McLaughlin, Kerry A.; Syme, Harriet M.; Graham, Peter; Catchpole, Brian

    2015-01-01

    Canine hypoadrenocorticism likely arises from immune-mediated destruction of adrenocortical tissue, leading to glucocorticoid and mineralocorticoid deficiency. In humans with autoimmune Addison’s disease (AAD) or autoimmune polyendocrine syndrome (APS), circulating autoantibodies have been demonstrated against enzymes associated with adrenal steroid synthesis. The current study investigates autoantibodies against steroid synthesis enzymes in dogs with spontaneous hypoadrenocorticism. Coding regions of canine CYP21A2 (21-hydroxylase; 21-OH), CYP17A1 (17-hydroxylase; 17-OH), CYP11A1 (P450 side-chain cleavage enzyme; P450scc) and HSD3B2 (3β hydroxysteroid dehydrogenase; 3βHSD) were amplified, cloned and expressed as 35S-methionine radiolabelled recombinant protein. In a pilot study, serum samples from 20 dogs with hypoadrenocorticism and four unaffected control dogs were screened by radio-immunoprecipitation assay. There was no evidence of reactivity against 21-OH, 17-OH or 3βHSD, but five dogs with hypoadrenocorticism showed immunoreactivity to P450scc compared with controls. Serum samples were subsequently obtained from 213 dogs diagnosed with hypoadrenocorticism and 110 dogs from a hospital control population. Thirty control dogs were randomly selected to establish a threshold for antibody positivity (mean + 3 × standard deviation). Dogs with hypoadrenocorticism were more likely to be P450scc autoantibody positive than hospital controls (24% vs. 1.2%, respectively; p = 0.0016). Sex was significantly associated with the presence of P450scc autoantibodies in the case population, with 30% of females testing positive compared with 17% of males (p = 0.037). Significant associations with breed (p = 0.015) and DLA-type (DQA1*006:01 allele; p = 0.017) were also found. This cross-sectional study indicates that P450scc autoantibodies are present in a proportion of dogs affected with hypoadrenocorticism. PMID:26618927

  13. Autoantibodies against Cytochrome P450 Side-Chain Cleavage Enzyme in Dogs (Canis lupus familiaris) Affected with Hypoadrenocorticism (Addison's Disease).

    PubMed

    Boag, Alisdair M; Christie, Michael R; McLaughlin, Kerry A; Syme, Harriet M; Graham, Peter; Catchpole, Brian

    2015-01-01

    Canine hypoadrenocorticism likely arises from immune-mediated destruction of adrenocortical tissue, leading to glucocorticoid and mineralocorticoid deficiency. In humans with autoimmune Addison's disease (AAD) or autoimmune polyendocrine syndrome (APS), circulating autoantibodies have been demonstrated against enzymes associated with adrenal steroid synthesis. The current study investigates autoantibodies against steroid synthesis enzymes in dogs with spontaneous hypoadrenocorticism. Coding regions of canine CYP21A2 (21-hydroxylase; 21-OH), CYP17A1 (17-hydroxylase; 17-OH), CYP11A1 (P450 side-chain cleavage enzyme; P450scc) and HSD3B2 (3β hydroxysteroid dehydrogenase; 3βHSD) were amplified, cloned and expressed as 35S-methionine radiolabelled recombinant protein. In a pilot study, serum samples from 20 dogs with hypoadrenocorticism and four unaffected control dogs were screened by radio-immunoprecipitation assay. There was no evidence of reactivity against 21-OH, 17-OH or 3βHSD, but five dogs with hypoadrenocorticism showed immunoreactivity to P450scc compared with controls. Serum samples were subsequently obtained from 213 dogs diagnosed with hypoadrenocorticism and 110 dogs from a hospital control population. Thirty control dogs were randomly selected to establish a threshold for antibody positivity (mean + 3 × standard deviation). Dogs with hypoadrenocorticism were more likely to be P450scc autoantibody positive than hospital controls (24% vs. 1.2%, respectively; p = 0.0016). Sex was significantly associated with the presence of P450scc autoantibodies in the case population, with 30% of females testing positive compared with 17% of males (p = 0.037). Significant associations with breed (p = 0.015) and DLA-type (DQA1*006:01 allele; p = 0.017) were also found. This cross-sectional study indicates that P450scc autoantibodies are present in a proportion of dogs affected with hypoadrenocorticism.

  14. "The Show"

    ERIC Educational Resources Information Center

    Gehring, John

    2004-01-01

    For the past 16 years, the blue-collar city of Huntington, West Virginia, has rolled out the red carpet to welcome young wrestlers and their families as old friends. They have come to town chasing the same dream for a spot in what many of them call "The Show". For three days, under the lights of an arena packed with 5,000 fans, the…

  15. Congenital lipoid adrenal hyperplasia

    PubMed Central

    2014-01-01

    Congenital lipoid adrenal hyperplasia (lipoid CAH) is the most fatal form of CAH, as it disrupts adrenal and gonadal steroidogenesis. Most cases of lipoid CAH are caused by recessive mutations in the gene encoding steroidogenic acute regulatory protein (StAR). Affected patients typically present with signs of severe adrenal failure in early infancy and 46,XY genetic males are phenotypic females due to disrupted testicular androgen secretion. The StAR p.Q258X mutation accounts for about 70% of affected alleles in most patients of Japanese and Korean ancestry. However, it is more prevalent (92.3%) in the Korean population. Recently, some patients have been showed that they had late and mild clinical findings. These cases and studies constitute a new entity of 'nonclassic lipoid CAH'. The cholesterol side-chain cleavage enzyme, P450scc (CYP11A1), plays an essential role converting cholesterol to pregnenolone. Although progesterone production from the fetally derived placenta is necessary to maintain a pregnancy to term, some patients with P450scc mutations have recently been reported. P450scc mutations can also cause lipoid CAH and establish a recently recognized human endocrine disorder. PMID:25654062

  16. A Proposed Molecular Mechanism of High-Dose Vitamin D3 Supplementation in Prevention and Treatment of Preeclampsia

    PubMed Central

    Zabul, Piotr; Wozniak, Michal; Slominski, Andrzej T.; Preis, Krzysztof; Gorska, Magdalena; Korozan, Marek; Wieruszewski, Jan; Zmijewski, Michal A.; Zabul, Ewa; Tuckey, Robert; Kuban-Jankowska, Alicja; Mickiewicz, Wieslawa; Knap, Narcyz

    2015-01-01

    A randomized prospective clinical study performed on a group of 74 pregnant women (43 presenting with severe preeclampsia) proved that urinary levels of 15-F2t-isoprostane were significantly higher in preeclamptic patients relative to the control (3.05 vs. 2.00 ng/mg creatinine). Surprisingly enough, plasma levels of 25-hydroxyvitamin D3 in both study groups were below the clinical reference range with no significant difference between the groups. In vitro study performed on isolated placental mitochondria and placental cell line showed that suicidal self-oxidation of cytochrome P450scc may lead to structural disintegration of heme, potentially contributing to enhancement of oxidative stress phenomena in the course of preeclampsia. As placental cytochrome P450scc pleiotropic activity is implicated in the metabolism of free radical mediated arachidonic acid derivatives as well as multiple Vitamin D3 hydroxylations and progesterone synthesis, we propose that Vitamin D3 might act as a competitive inhibitor of placental cytochrome P450scc preventing the production of lipid peroxides or excess progesterone synthesis, both of which may contribute to the etiopathogenesis of preeclampsia. The proposed molecular mechanism is in accord with the preliminary clinical observations on the surprisingly high efficacy of high-dose Vitamin D3 supplementation in prevention and treatment of preeclampsia. PMID:26068234

  17. Effects of 17alpha-methyltestosterone exposure on steroidogenesis and cyclin-B mRNA expression in previtellogenic oocytes of Atlantic cod (Gadus morhua).

    PubMed

    Kortner, Trond M; Arukwe, Augustine

    2007-11-01

    Steroid hormone (estrogens and androgens) synthesis and regulation involve a large number of enzymes and potential biochemical pathways. In the context of these biochemical pathways, it is believed that the true rate-limiting step in acute steroid production is the movement of cholesterol across the mitochondrial membrane by the steroidogenic acute regulatory (StAR) protein and the subsequent conversion to pregnenolone by cytochrome P450-mediated side-chain cleavage (P450scc) enzyme. Oocyte development is a complex process that is triggered by the maturation-promoting factor (MPF) involving cyclin-B as a regulatory factor. In the present study, we evaluated the endocrine effects of 17alpha-methyltestosterone (MT) on steroidogenic pathways of Atlantic cod (Gadus morhua), using an in vitro previtellogenic oocyte culture technique that is based on an agarose floating method. Tissue was cultured in a humidified incubator at 10 degrees C for 1, 5, 10 and 20 days with different concentrations of the synthetic androgen MT (0 (control), 1, 10, 100 and 1000 microM) dissolved in ethanol (0.3%). Gene expressions for StAR, P450scc, aromatase-alpha (P450aromA) and cyclin-B were detected using validated real-time PCR with specific primer pairs. Cellular localization of the StAR protein and P450scc were performed using the immunohistochemical technique with antisera prepared against synthetic peptide for both proteins. Steroid hormones (estradiol-17beta: E2 and testosterone: T) levels were estimated using enzyme immunoassay. Our data showed significant concentration-specific increase (at day 1 and 5) and decrease (at day 10 and 20) of the StAR mRNA expression after exposure to MT. P450scc expression showed a MT concentration-specific decrease during the exposure periods and cyclin-B mRNA expression was decreased in MT concentration-dependent manner at days 10 and 20 (reaching almost total inhibition after exposure to 1000 microM MT). MT exposure produced variable effects on the P

  18. Regulation by retinoids of luteinizing hormone/chorionic gonadotropin receptor, cholesterol side-chain cleavage cytochrome P-450, 3 beta-hydroxysteroid dehydrogenase/delta (5-4)-isomerase and 17 alpha-hydroxylase/C17-20 lyase cytochrome P-450 messenger ribonucleic acid levels in the K9 mouse Leydig cell line.

    PubMed

    Lefèvre, A; Rogier, E; Astraudo, C; Duquenne, C; Finaz, C

    1994-12-01

    Vitamin A is a potent regulator of testicular function. We have reported that retinol (R) and retinoic acid (RA) induced a down regulation of luteinizing hormone/human chorionic gonadotropin (LH/CG) binding sites in K9 Leydig cells. In the present study we evaluated the effect of R and RA on LH/CG receptors, cholesterol side-chain cleavage cytochrome P-450 (P-450 scc), 17 alpha-hydroxylase/C17-20 lyase (P-450 17 alpha) and 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) mRNA levels in K9 mouse Leydig cells. To validate K9 cells as a model for studying Leydig cell steroidogenesis at the molecular level, we first investigated the effect of hCG on mRNA levels of the steroidogenic enzymes. P-450 scc, 3 beta HSD and P-450 17 alpha were expressed constitutively. The addition of 10 ng/ml hCG enhanced mRNA levels for the three genes within 2 h. Maximal accumulation of P-450 scc, P-450 17 alpha and 3 beta HSD mRNA in treated cells represents a 2.5-, 8.5- and 4-fold increase over control values, respectively. P-450 17 alpha expression reached a maximum by 4 h and then declined rapidly to return to control value by 24 h. The pattern of LH/CG receptor mRNAs in K9 cells was very similar to that of MA10 Leydig cells and showed six transcripts of 1.1, 1.6, 1.9, 2.6, 4.2 and 7.0 kb. Treatment of cells with R or RA resulted in a time- and dose-dependent decrease in all six species.(ABSTRACT TRUNCATED AT 250 WORDS)

  19. Effects of dietary soybean isoflavones (SI) on reproduction in the young breeder rooster.

    PubMed

    Heng, Dai; Zhang, Tao; Tian, Ye; Yu, Shangyu; Liu, Wenbo; Xu, Kaili; Liu, Juan; Ding, Yu; Zhu, Baochang; Yang, Yanzhou; Zhang, Cheng

    2017-02-01

    Soybean isoflavones (SIs) are phytoestrogens that competitive with estrogens in body. Although SIs play an important role in reproduction, their role in testicular development in roosters is unknown. This study was conducted to investigate the effect of SIs on testicular development and serum reproductive hormone profiles in young breeder roosters (70-133days old). Gene expression of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc), and 3β-hydroxysteroid dehydrogenase (3β-HSD), which are related to testosterone synthesis, in rooster testis were also evaluated after treatment with different SI doses. Although SIs had no significant effect on body weight, 5mg/kg SIs significantly increased the testis index and serum levels of reproductive hormones (gonadotropin releasing hormone, follicle- stimulating hormone, luteinizing hormone, and testosterone).To further investigate whether SIs regulate hormone synthesis via StAR, p450scc, 3β-HSD, real time-PCR was performed to measure the mRNA levels of the corresponding genes. The results showed that 5mg/kg of SIs significantly increased StAR mRNA levels. However, there were no significant effects on p450scc or 3β-HSD mRNA levels. Moreover, the spermatogonial development and the number of germ cell layers were increased by treatment with 5mg/kg of SIs. These results suggest that SIs promote testicular growth by increasing reproductive hormone secretion, which is closely related to StAR expression, to positively regulate reproduction in young roosters.

  20. T-2 toxin inhibits gene expression and activity of key steroidogenesis enzymes in mouse Leydig cells.

    PubMed

    Yang, Jian Ying; Zhang, Yong Fa; Meng, Xiang Ping; Li, Yuan Xiao; Ma, Kai Wang; Bai, Xue Fei

    2015-08-01

    T-2 toxin is one of the mycotoxins, a group of type A trichothecenes produced by several fungal genera including Fusarium species, which may lead to the decrease of the testosterone secretion in the primary Leydig cells derived from the mouse testis. The previous study demonstrated the effects of T-2 toxin through direct decrease of the testosterone biosynthesis in the primary Leydig cells derived from the mouse testis. In this study, we further examined the direct biological effects of T-2 toxin on steroidogenesis production, primarily in Leydig cells of mice. Mature mouse Leydig cells were purified by Percoll gradient centrifugation and the cell purity was determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining. To examine T-2 toxin-induced testosterone secretion decrease, we measured the transcription levels of 3 key steroidogenic enzymes and 5 enzyme activities including 3β-HSD-1, P450scc, StAR, CYP17A1, and 17β-HSD in T-2 toxin/human chorionicgonadotropin (hCG) co-treated cells. Our previous study showed that T-2 toxin (10(-7) M, 10(-8) M and 10(-9) M) significantly suppressed hCG (10 ng/ml)-induced testosterone secretion. The studies demonstrated that the suppressive effect is correlated with the decreases in the levels of transcription of 3β-HSD-1, P450scc, and StAR (P<0.05) and also in enzyme activities of 3β-HSD-1, P450scc, StAR, CYP17A1, and 17β-HSD (P<0.05).

  1. Cumene hydroperoxide supported demethylation of N,N-dimethylaniline by cytochrome P-450 from adrenal cortex mitochondria.

    PubMed

    Akhrem, A A; Khatyleva SYu; Shkumatov, V M; Chashchin, V L; Kiselev, P A

    1982-01-01

    The interaction of highly purified cytochrome P-450 from bovine adrenal cortex mitochondria (cytochrome P-450scc) with N,N-dimethylaniline (DMA), aniline, N-dimethylcyclohexylamine and cumene hydroperoxide (CHP) has been investigated. The formation of complexes between cytochrome P-450scc and the above listed compounds could be demonstrated. The reaction of oxidative demethylation of DMA by cumene hydroperoxide involving cytochrome P-450scc has been carried out at 37 degrees C; the mechanism of this process is discussed. Incubation of cytochrome P-450scc with negatively charged phospholipids, phosphatidylglycerol (PG), and phosphatidylinosite (PI) exerts an inhibiting effect on the reaction of oxidative demethylation. The interaction of cytochrome P-450scc with CHP is accompanied by hemoprotein destruction in a complex biphasic way. The process of oxidative demethylation of DMA in the system of cytochrome P-450scc-CHP has been concluded to have a predominantly radical character.

  2. Television Quiz Show Simulation

    ERIC Educational Resources Information Center

    Hill, Jonnie Lynn

    2007-01-01

    This article explores the simulation of four television quiz shows for students in China studying English as a foreign language (EFL). It discusses the adaptation and implementation of television quiz shows and how the students reacted to them.

  3. Showing What They Know

    ERIC Educational Resources Information Center

    Cech, Scott J.

    2008-01-01

    Having students show their skills in three dimensions, known as performance-based assessment, dates back at least to Socrates. Individual schools such as Barrington High School--located just outside of Providence--have been requiring students to actively demonstrate their knowledge for years. The Rhode Island's high school graduating class became…

  4. The Ozone Show.

    ERIC Educational Resources Information Center

    Mathieu, Aaron

    2000-01-01

    Uses a talk show activity for a final assessment tool for students to debate about the ozone hole. Students are assessed on five areas: (1) cooperative learning; (2) the written component; (3) content; (4) self-evaluation; and (5) peer evaluation. (SAH)

  5. What Do Maps Show?

    ERIC Educational Resources Information Center

    Geological Survey (Dept. of Interior), Reston, VA.

    This curriculum packet, appropriate for grades 4-8, features a teaching poster which shows different types of maps (different views of Salt Lake City, Utah), as well as three reproducible maps and reproducible activity sheets which complement the maps. The poster provides teacher background, including step-by-step lesson plans for four geography…

  6. Show Me the Way

    ERIC Educational Resources Information Center

    Dicks, Matthew J.

    2005-01-01

    Because today's students have grown up steeped in video games and the Internet, most of them expect feedback, and usually gratification, very soon after they expend effort on a task. Teachers can get quick feedback to students by showing them videotapes of their learning performances. The author, a 3rd grade teacher describes how the seemingly…

  7. Chemistry Game Shows

    NASA Astrophysics Data System (ADS)

    Campbell, Susan; Muzyka, Jennifer

    2002-04-01

    We present a technological improvement to the use of game shows to help students review for tests. Our approach uses HTML files interpreted with a browser on a computer attached to an LCD projector. The HTML files can be easily modified for use of the game in a variety of courses.

  8. Honored Teacher Shows Commitment.

    ERIC Educational Resources Information Center

    Ratte, Kathy

    1987-01-01

    Part of the acceptance speech of the 1985 National Council for the Social Studies Teacher of the Year, this article describes the censorship experience of this honored social studies teacher. The incident involved the showing of a videotape version of the feature film entitled "The Seduction of Joe Tynan." (JDH)

  9. Talk Show Science.

    ERIC Educational Resources Information Center

    Moore, Mitzi Ruth

    1992-01-01

    Proposes having students perform skits in which they play the roles of the science concepts they are trying to understand. Provides the dialog for a skit in which hot and cold gas molecules are interviewed on a talk show to study how these properties affect wind, rain, and other weather phenomena. (MDH)

  10. Stage a Water Show

    ERIC Educational Resources Information Center

    Frasier, Debra

    2008-01-01

    In the author's book titled "The Incredible Water Show," the characters from "Miss Alaineus: A Vocabulary Disaster" used an ocean of information to stage an inventive performance about the water cycle. In this article, the author relates how she turned the story into hands-on science teaching for real-life fifth-grade students. The author also…

  11. Not a "reality" show.

    PubMed

    Wrong, Terence; Baumgart, Erica

    2013-01-01

    The authors of the preceding articles raise legitimate questions about patient and staff rights and the unintended consequences of allowing ABC News to film inside teaching hospitals. We explain why we regard their fears as baseless and not supported by what we heard from individuals portrayed in the filming, our decade-long experience making medical documentaries, and the full un-aired context of the scenes shown in the broadcast. The authors don't and can't know what conversations we had, what documents we reviewed, and what protections we put in place in each televised scene. Finally, we hope to correct several misleading examples cited by the authors as well as their offhand mischaracterization of our program as a "reality" show.

  12. Public medical shows.

    PubMed

    Walusinski, Olivier

    2014-01-01

    In the second half of the 19th century, Jean-Martin Charcot (1825-1893) became famous for the quality of his teaching and his innovative neurological discoveries, bringing many French and foreign students to Paris. A hunger for recognition, together with progressive and anticlerical ideals, led Charcot to invite writers, journalists, and politicians to his lessons, during which he presented the results of his work on hysteria. These events became public performances, for which physicians and patients were transformed into actors. Major newspapers ran accounts of these consultations, more like theatrical shows in some respects. The resultant enthusiasm prompted other physicians in Paris and throughout France to try and imitate them. We will compare the form and substance of Charcot's lessons with those given by Jules-Bernard Luys (1828-1897), Victor Dumontpallier (1826-1899), Ambroise-Auguste Liébault (1823-1904), Hippolyte Bernheim (1840-1919), Joseph Grasset (1849-1918), and Albert Pitres (1848-1928). We will also note their impact on contemporary cinema and theatre.

  13. The Great Cometary Show

    NASA Astrophysics Data System (ADS)

    2007-01-01

    its high spatial and spectral resolution, it was possible to zoom into the very heart of this very massive star. In this innermost region, the observations are dominated by the extremely dense stellar wind that totally obscures the underlying central star. The AMBER observations show that this dense stellar wind is not spherically symmetric, but exhibits a clearly elongated structure. Overall, the AMBER observations confirm that the extremely high mass loss of Eta Carinae's massive central star is non-spherical and much stronger along the poles than in the equatorial plane. This is in agreement with theoretical models that predict such an enhanced polar mass-loss in the case of rapidly rotating stars. ESO PR Photo 06c/07 ESO PR Photo 06c/07 RS Ophiuchi in Outburst Several papers from this special feature focus on the later stages in a star's life. One looks at the binary system Gamma 2 Velorum, which contains the closest example of a star known as a Wolf-Rayet. A single AMBER observation allowed the astronomers to separate the spectra of the two components, offering new insights in the modeling of Wolf-Rayet stars, but made it also possible to measure the separation between the two stars. This led to a new determination of the distance of the system, showing that previous estimates were incorrect. The observations also revealed information on the region where the winds from the two stars collide. The famous binary system RS Ophiuchi, an example of a recurrent nova, was observed just 5 days after it was discovered to be in outburst on 12 February 2006, an event that has been expected for 21 years. AMBER was able to detect the extension of the expanding nova emission. These observations show a complex geometry and kinematics, far from the simple interpretation of a spherical fireball in extension. AMBER has detected a high velocity jet probably perpendicular to the orbital plane of the binary system, and allowed a precise and careful study of the wind and the shockwave

  14. Stretched View Showing 'Victoria'

    NASA Technical Reports Server (NTRS)

    2006-01-01

    [figure removed for brevity, see original site] Stretched View Showing 'Victoria'

    This pair of images from the panoramic camera on NASA's Mars Exploration Rover Opportunity served as initial confirmation that the two-year-old rover is within sight of 'Victoria Crater,' which it has been approaching for more than a year. Engineers on the rover team were unsure whether Opportunity would make it as far as Victoria, but scientists hoped for the chance to study such a large crater with their roving geologist. Victoria Crater is 800 meters (nearly half a mile) in diameter, about six times wider than 'Endurance Crater,' where Opportunity spent several months in 2004 examining rock layers affected by ancient water.

    When scientists using orbital data calculated that they should be able to detect Victoria's rim in rover images, they scrutinized frames taken in the direction of the crater by the panoramic camera. To positively characterize the subtle horizon profile of the crater and some of the features leading up to it, researchers created a vertically-stretched image (top) from a mosaic of regular frames from the panoramic camera (bottom), taken on Opportunity's 804th Martian day (April 29, 2006).

    The stretched image makes mild nearby dunes look like more threatening peaks, but that is only a result of the exaggerated vertical dimension. This vertical stretch technique was first applied to Viking Lander 2 panoramas by Philip Stooke, of the University of Western Ontario, Canada, to help locate the lander with respect to orbiter images. Vertically stretching the image allows features to be more readily identified by the Mars Exploration Rover science team.

    The bright white dot near the horizon to the right of center (barely visible without labeling or zoom-in) is thought to be a light-toned outcrop on the far wall of the crater, suggesting that the rover can see over the low rim of Victoria. In figure 1, the northeast and southeast rims are labeled

  15. Areolae of the placenta in the Antarctic minke whale (Balaenoptera bonaerensis).

    PubMed

    Sasaki, Motoki; Amano, Yoko; Hayakawa, Daisuke; Tsubota, Toshio; Ishikawa, Hajime; Mogoe, Toshihiro; Ohsumi, Seiji; Tetsuka, Masafumi; Miyamoto, Akio; Fukui, Yutaka; Budipitojo, Teguh; Kitamura, Nobuo

    2014-03-07

    In this study, we examined the existence and structure of areolae and the steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta morphologically and immunohistochemically. Placentas were collected from the 15th, 16th and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were recognized in observations of serial sections. The opening of the areolar cavity was hidden by chorionic villi with areolar trophoblast cells. Furthermore, a closed pouch-like structure lined by tall columnar cells similar to areolar trophoblast cells within the stroma of chorionic villi was noticed and continued to the areolar cavity, with the opening seen on serial sections. In a surface investigation of the chorion and endometrium by SEM, maternal (endometrial) areolae irregularly surrounded by endometrial folds were obvious. Moreover, we distinguished areolar trophoblast cells with long microvilli attached with many blebs from trophoblast cells. In our immunohistochemical observations, a steroidogenic enzyme, cytochrome P450 side chain cleavage enzyme (P450scc), was detected with strong immunoreactivity in trophoblast cells. However, areolar trophoblast cells showed weak or no immunoreactivity for P450scc.

  16. Abnormal regulation for progesterone production in placenta with prenatal cocaine exposure in rats.

    PubMed

    Wu, L; Yan, J; Qu, S C; Feng, Y Q; Jiang, X L

    2012-12-01

    Cocaine abuse in pregnant women is currently a significant public hygiene problem and is tightly associated with elevated risk for preterm delivery. Placental steroidogenesis especially progesterone production was essential for success and maintenance of pregnancy in humans and rodents. In the present study, we determined the impact of prenatal cocaine exposure on pathways of placental progesterone synthesis in rats. Pregnant rats were treated cocaine twice daily (15 mg/kg/day) during the third trimester, and the maternal and fetal plasma progesterone and pregnenolone concentrations were detected. We also examined both the protein and mRNA expression of some key enzymes and regulators for progesterone production in placenta. Results showed that, after maternal cocaine use during pregnancy, progesterone and pregnenolone concentrations in both maternal and fetal rats were significantly decreased. Although prenatal cocaine exposure had no effects on placental 3β-hydroxysteroid dehydrogenase type 1 (3βHSD1) expression, protein and mRNA expression of the cholesterol side-chain cleavage enzyme (P450scc/CYP11a) in placenta was significantly inhibited. Moreover, protein and mRNA expressions of MLN64 that regulating cholesterol transport and activating protein 2γ (AP2γ/Tfap2c) that controlling P450scc/CYP11a gene expression in placenta were both decreased following maternal cocaine use in pregnancy. Collectively, this study suggested that prenatal cocaine exposure could insult the placental progesterone production in rats possibly associated with the high risk for preterm delivery.

  17. Effects of T-2 toxin on the regulation of steroidogenesis in mouse Leydig cells.

    PubMed

    Yang, Jian Ying; Zhang, Yong Fa; Li, Yuan Xiao; Guan, Gui Ping; Kong, Xiang Feng; Liang, Ai Min; Ma, Kai Wang; Da Li, Guang; Bai, Xue Fei

    2016-10-01

    T-2 toxin is one of the mycotoxins, a group of type A trichothecenes produced by several fungal genera including Fusarium species, which may lead to the decrease of testosterone secretion in primary Leydig cells derived from mouse testis. The previous study demonstrated T-2 toxin decrease the testosterone biosynthesis in the primary Leydig cells derived from the mouse testis directly. In this study, we further examined the direct biological effects of T-2 toxin on the process of steroidogenesis, primarily in Leydig cells of mice. Leydig cells of mature mouse were purified by Percoll gradient centrifugation and the cell purity was determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining. To examine the decrease in T-2 toxin-induced testosterone secretion, we measured the transcription level of three key steroidogenic enzymes including 3β-HSD-1, cytochrome P450 side-chain cleavage (P450scc) enzyme, and steroidogenic acute regulatory (StAR) protein in T-2 toxin/human chorionic gonadotropin (hCG) co-treated cells. Our previous study showed that T-2 toxin (10(-7), 10(-8), and 10(-9) M) significantly suppressed hCG (10 ng/ml)-induced testosterone secretion. The studies demonstrated that the suppressive effect is correlated with a decrease in the level of transcription of 3β-HSD-1, P450scc, and StAR (p < 0.05).

  18. Regulation of mRNAs encoding the steroidogenic acute regulatory protein and cholesterol side-chain cleavage enzyme in the elasmobranch interrenal gland.

    PubMed

    Evans, Andrew N; Nunez, B Scott

    2010-08-01

    The rate-limiting and regulated step in steroidogenesis, the conversion of cholesterol to pregnenolone, is facilitated by the steroidogenic acute regulatory protein (StAR) and cytochrome P450 cholesterol side-chain cleavage (P450scc). We have isolated cDNAs encoding StAR and P450scc from the Atlantic stingray, Dasyatis sabina, and characterized the steroidogenic activity of the encoded proteins using a heterologous expression system. Green monkey kidney (COS-1) cells cotransfected with D. sabina StAR and human P450scc/adrenodoxin reductase/adrenodoxin fusion (F2) constructs produced significantly more pregnenolone than cells transfected with the F2 construct alone. COS-1 cells transfected with a modified F2 construct (F2DS) in which human P450scc is replaced by D. sabina P450scc had higher rates than cells transfected with D. sabina P450scc alone. In other vertebrates, the stress peptide adrenocorticotropic hormone (ACTH) elicits its effects on corticosteroidogenesis in part through regulation of StAR and P450scc mRNAs. In vitro incubation of D. sabina interrenal tissue with porcine ACTH significantly increased intracellular cAMP and corticosteroid production. As demonstrated by quantitative PCR, ACTH also induced significant increases in mRNA abundance of both StAR and P450scc. Our results suggest that, as in higher vertebrates, chronic ACTH-induced glucocorticoid synthesis in elasmobranchs is mediated by regulation of primary steroidogenic mRNAs. This study is the first to demonstrate steroidogenic activity of an elasmobranch P450scc protein and express a composite elasmobranch steroidogenic pathway in a heterologous cell line. Also, the regulation of StAR and P450scc mRNAs has not previously been demonstrated in elasmobranch fishes.

  19. 15. Detail showing lower chord pinconnected to vertical member, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Detail showing lower chord pin-connected to vertical member, showing floor beam riveted to extension of vertical member below pin-connection, and showing brackets supporting cantilevered sidewalk. View to southwest. - Selby Avenue Bridge, Spanning Short Line Railways track at Selby Avenue between Hamline & Snelling Avenues, Saint Paul, Ramsey County, MN

  20. Sequential metabolism of 7-dehydrocholesterol to steroidal 5,7-dienes in adrenal glands and its biological implication in the skin.

    PubMed

    Slominski, Andrzej T; Zmijewski, Michal A; Semak, Igor; Sweatman, Trevor; Janjetovic, Zorica; Li, Wei; Zjawiony, Jordan K; Tuckey, Robert C

    2009-01-01

    Since P450scc transforms 7-dehydrocholesterol (7DHC) to 7-dehydropregnenolone (7DHP) in vitro, we investigated sequential 7DHC metabolism by adrenal glands ex vivo. There was a rapid, time- and dose-dependent metabolism of 7DHC by adrenals from rats, pigs, rabbits and dogs with production of more polar 5,7-dienes as detected by RP-HPLC. Based on retention time (RT), UV spectra and mass spectrometry, we identified the major products common to all tested species as 7DHP, 22-hydroxy-7DHC and 20,22-dihydroxy-7DHC. The involvement of P450scc in adrenal metabolic transformation was confirmed by the inhibition of this process by DL-aminoglutethimide. The metabolism of 7DHC with subsequent production of 7DHP was stimulated by forscolin indicating involvement of cAMP dependent pathways. Additional minor products of 7DHC metabolism that were more polar than 7DHP were identified as 17-hydroxy-7DHP (in pig adrenals but not those of rats) and as pregna-4,7-diene-3,20-dione (7-dehydroprogesterone). Both products represented the major identifiable products of 7DHP metabolism in adrenal glands. Studies with purified enzymes show that StAR protein likely transports 7DHC to the inner mitochondrial membrane, that 7DHC can compete effectively with cholesterol for the substrate binding site on P450scc and that the catalytic efficiency of 3betaHSD for 7DHP (V(m)/K(m)) is 40% of that for pregnenolone. Skin mitochondria are capable of transforming 7DHC to 7DHP and the 7DHP is metabolized further by skin extracts. Finally, 7DHP, its photoderivative 20-oxopregnacalciferol, and pregnenolone exhibited biological activity in skin cells including inhibition of proliferation of epidermal keratinocytes and melanocytes, and melanoma cells. These findings define a novel steroidogenic pathway: 7DHC-->22(OH)7DHC-->20,22(OH)(2)7DHC-->7DHP, with potential further metabolism of 7DHP mediated by 3betaHSD or CYP17, depending on mammalian species. The 5-7 dienal intermediates of the pathway can be a source

  1. Sequential Metabolism of 7-Dehydrocholesterol to Steroidal 5,7-Dienes in Adrenal Glands and Its Biological Implication in the Skin

    PubMed Central

    Slominski, Andrzej T.; Zmijewski, Michal A.; Semak, Igor; Sweatman, Trevor; Janjetovic, Zorica; Li, Wei; Zjawiony, Jordan K.; Tuckey, Robert C.

    2009-01-01

    Since P450scc transforms 7-dehydrocholesterol (7DHC) to 7-dehydropregnenolone (7DHP) in vitro, we investigated sequential 7DHC metabolism by adrenal glands ex vivo. There was a rapid, time- and dose-dependent metabolism of 7DHC by adrenals from rats, pigs, rabbits and dogs with production of more polar 5,7-dienes as detected by RP-HPLC. Based on retention time (RT), UV spectra and mass spectrometry, we identified the major products common to all tested species as 7DHP, 22-hydroxy-7DHC and 20,22-dihydroxy-7DHC. The involvement of P450scc in adrenal metabolic transformation was confirmed by the inhibition of this process by DL-aminoglutethimide. The metabolism of 7DHC with subsequent production of 7DHP was stimulated by forscolin indicating involvement of cAMP dependent pathways. Additional minor products of 7DHC metabolism that were more polar than 7DHP were identified as 17-hydroxy-7DHP (in pig adrenals but not those of rats) and as pregna-4,7-diene-3,20-dione (7-dehydroprogesterone). Both products represented the major identifiable products of 7DHP metabolism in adrenal glands. Studies with purified enzymes show that StAR protein likely transports 7DHC to the inner mitochondrial membrane, that 7DHC can compete effectively with cholesterol for the substrate binding site on P450scc and that the catalytic efficiency of 3βHSD for 7DHP (Vm/Km) is 40% of that for pregnenolone. Skin mitochondria are capable of transforming 7DHC to 7DHP and the 7DHP is metabolized further by skin extracts. Finally, 7DHP, its photoderivative 20-oxopregnacalciferol, and pregnenolone exhibited biological activity in skin cells including inhibition of proliferation of epidermal keratinocytes and melanocytes, and melanoma cells. These findings define a novel steroidogenic pathway: 7DHC→22(OH)7DHC→20,22(OH)27DHC→7DHP, with potential further metabolism of 7DHP mediated by 3βHSD or CYP17, depending on mammalian species. The 5–7 dienal intermediates of the pathway can be a source of

  2. Hey Teacher, Your Personality's Showing!

    ERIC Educational Resources Information Center

    Paulsen, James R.

    1977-01-01

    A study of 30 fourth, fifth, and sixth grade teachers and 300 of their students showed that a teacher's age, sex, and years of experience did not relate to students' mathematics achievement, but that more effective teachers showed greater "freedom from defensive behavior" than did less effective teachers. (DT)

  3. Planning a Successful Tech Show

    ERIC Educational Resources Information Center

    Nikirk, Martin

    2011-01-01

    Tech shows are a great way to introduce prospective students, parents, and local business and industry to a technology and engineering or career and technical education program. In addition to showcasing instructional programs, a tech show allows students to demonstrate their professionalism and skills, practice public presentations, and interact…

  4. Satellite Animation Shows California Storms

    NASA Video Gallery

    This animation of visible and infrared imagery from NOAA's GOES-West satellite shows a series of moisture-laden storms affecting California from Jan. 6 through Jan. 9, 2017. TRT: 00:36 Credit: NASA...

  5. Satellite Movie Shows Erika Dissipate

    NASA Video Gallery

    This animation of visible and infrared imagery from NOAA's GOES-West satellite from Aug. 27 to 29 shows Tropical Storm Erika move through the Eastern Caribbean Sea and dissipate near eastern Cuba. ...

  6. National Orange Show Photovoltaic Demonstration

    SciTech Connect

    Dan Jimenez Sheri Raborn, CPA; Tom Baker

    2008-03-31

    National Orange Show Photovoltaic Demonstration created a 400KW Photovoltaic self-generation plant at the National Orange Show Events Center (NOS). The NOS owns a 120-acre state fairground where it operates an events center and produces an annual citrus fair known as the Orange Show. The NOS governing board wanted to employ cost-saving programs for annual energy expenses. It is hoped the Photovoltaic program will result in overall savings for the NOS, help reduce the State's energy demands as relating to electrical power consumption, improve quality of life within the affected grid area as well as increase the energy efficiency of buildings at our venue. In addition, the potential to reduce operational expenses would have a tremendous effect on the ability of the NOS to service its community.

  7. Phyllodes tumor showing intraductal growth.

    PubMed

    Makidono, Akari; Tsunoda, Hiroko; Mori, Miki; Yagata, Hiroshi; Onoda, Yui; Kikuchi, Mari; Nozaki, Taiki; Saida, Yukihisa; Nakamura, Seigo; Suzuki, Koyu

    2013-07-01

    Phyllodes tumor of the breast is a rare fibroepithelial lesion and particularly uncommon in adolescent girls. It is thought to arise from the periductal rather than intralobular stroma. Usually, it is seen as a well-defined mass. Phyllodes tumor showing intraductal growth is extremely rare. Here we report a girl who has a phyllodes tumor with intraductal growth.

  8. Magic Carpet Shows Its Colors

    NASA Technical Reports Server (NTRS)

    2004-01-01

    The upper left image in this display is from the panoramic camera on the Mars Exploration Rover Spirit, showing the 'Magic Carpet' region near the rover at Gusev Crater, Mars, on Sol 7, the seventh martian day of its journey (Jan. 10, 2004). The lower image, also from the panoramic camera, is a monochrome (single filter) image of a rock in the 'Magic Carpet' area. Note that colored portions of the rock correlate with extracted spectra shown in the plot to the side. Four different types of materials are shown: the rock itself, the soil in front of the rock, some brighter soil on top of the rock, and some dust that has collected in small recesses on the rock face ('spots'). Each color on the spectra matches a line on the graph, showing how the panoramic camera's different colored filters are used to broadly assess the varying mineral compositions of martian rocks and soils.

  9. "Medicine show." Alice in Doctorland.

    PubMed

    1987-01-01

    This is an excerpt from the script of a 1939 play provided to the Institute of Social Medicine and Community Health by the Library of Congress Federal Theater Project Collection at George Mason University Library, Fairfax, Virginia, pages 2-1-8 thru 2-1-14. The Federal Theatre Project (FTP) was part of the New Deal program for the arts 1935-1939. Funded by the Works Progress Administration (WPA) its goal was to employ theater professionals from the relief rolls. A number of FTP plays deal with aspects of medicine and public health. Pageants, puppet shows and documentary plays celebrated progress in medical science while examining social controversies in medical services and the public health movement. "Medicine Show" sharply contrasts technological wonders with social backwardness. The play was rehearsed by the FTP but never opened because funding ended. A revised version ran on Broadway in 1940. The preceding comments are adapted from an excellent, well-illustrated review of five of these plays by Barabara Melosh: "The New Deal's Federal Theatre Project," Medical Heritage, Vol. 2, No. 1 (Jan/Feb 1986), pp. 36-47.

  10. "Show me" bioethics and politics.

    PubMed

    Christopher, Myra J

    2007-10-01

    Missouri, the "Show Me State," has become the epicenter of several important national public policy debates, including abortion rights, the right to choose and refuse medical treatment, and, most recently, early stem cell research. In this environment, the Center for Practical Bioethics (formerly, Midwest Bioethics Center) emerged and grew. The Center's role in these "cultural wars" is not to advocate for a particular position but to provide well researched and objective information, perspective, and advocacy for the ethical justification of policy positions; and to serve as a neutral convener and provider of a public forum for discussion. In this article, the Center's work on early stem cell research is a case study through which to argue that not only the Center, but also the field of bioethics has a critical role in the politics of public health policy.

  11. Phoenix Scoop Inverted Showing Rasp

    NASA Technical Reports Server (NTRS)

    2008-01-01

    This image taken by the Surface Stereo Imager on Sol 49, or the 49th Martian day of the mission (July 14, 2008), shows the silver colored rasp protruding from NASA's Phoenix Mars Lander's Robotic Arm scoop. The scoop is inverted and the rasp is pointing up.

    Shown with its forks pointing toward the ground is the thermal and electrical conductivity probe, at the lower right. The Robotic Arm Camera is pointed toward the ground.

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is led by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  12. ShowMe3D

    SciTech Connect

    Sinclair, Michael B

    2012-01-05

    ShowMe3D is a data visualization graphical user interface specifically designed for use with hyperspectral image obtained from the Hyperspectral Confocal Microscope. The program allows the user to select and display any single image from a three dimensional hyperspectral image stack. By moving a slider control, the user can easily move between images of the stack. The user can zoom into any region of the image. The user can select any pixel or region from the displayed image and display the fluorescence spectrum associated with that pixel or region. The user can define up to 3 spectral filters to apply to the hyperspectral image and view the image as it would appear from a filter-based confocal microscope. The user can also obtain statistics such as intensity average and variance from selected regions.

  13. Casimir experiments showing saturation effects

    SciTech Connect

    Sernelius, Bo E.

    2009-10-15

    We address several different Casimir experiments where theory and experiment disagree. First out is the classical Casimir force measurement between two metal half spaces; here both in the form of the torsion pendulum experiment by Lamoreaux and in the form of the Casimir pressure measurement between a gold sphere and a gold plate as performed by Decca et al.; theory predicts a large negative thermal correction, absent in the high precision experiments. The third experiment is the measurement of the Casimir force between a metal plate and a laser irradiated semiconductor membrane as performed by Chen et al.; the change in force with laser intensity is larger than predicted by theory. The fourth experiment is the measurement of the Casimir force between an atom and a wall in the form of the measurement by Obrecht et al. of the change in oscillation frequency of a {sup 87}Rb Bose-Einstein condensate trapped to a fused silica wall; the change is smaller than predicted by theory. We show that saturation effects can explain the discrepancies between theory and experiment observed in all these cases.

  14. Mimas Showing False Colors #1

    NASA Technical Reports Server (NTRS)

    2005-01-01

    False color images of Saturn's moon, Mimas, reveal variation in either the composition or texture across its surface.

    During its approach to Mimas on Aug. 2, 2005, the Cassini spacecraft narrow-angle camera obtained multi-spectral views of the moon from a range of 228,000 kilometers (142,500 miles).

    The image at the left is a narrow angle clear-filter image, which was separately processed to enhance the contrast in brightness and sharpness of visible features. The image at the right is a color composite of narrow-angle ultraviolet, green, infrared and clear filter images, which have been specially processed to accentuate subtle changes in the spectral properties of Mimas' surface materials. To create this view, three color images (ultraviolet, green and infrared) were combined into a single black and white picture that isolates and maps regional color differences. This 'color map' was then superimposed over the clear-filter image at the left.

    The combination of color map and brightness image shows how the color differences across the Mimas surface materials are tied to geological features. Shades of blue and violet in the image at the right are used to identify surface materials that are bluer in color and have a weaker infrared brightness than average Mimas materials, which are represented by green.

    Herschel crater, a 140-kilometer-wide (88-mile) impact feature with a prominent central peak, is visible in the upper right of each image. The unusual bluer materials are seen to broadly surround Herschel crater. However, the bluer material is not uniformly distributed in and around the crater. Instead, it appears to be concentrated on the outside of the crater and more to the west than to the north or south. The origin of the color differences is not yet understood. It may represent ejecta material that was excavated from inside Mimas when the Herschel impact occurred. The bluer color of these materials may be caused by subtle differences in

  15. The modulatory role of transforming growth factor beta1 and androstenedione on follicle-stimulating hormone-induced gelatinase secretion and steroidogenesis in rat granulosa cells.

    PubMed

    Ke, Ferng-Chun; Chuang, Li-Chung; Lee, Ming-Ting; Chen, Yun Ju; Lin, Sui-Wen; Wang, Paulus S; Stocco, Douglas M; Hwang, Jiuan-Jiuan

    2004-05-01

    To investigate the potential roles of matrix metalloproteinases (MMPs) in ovarian granulosa cell differentiation, we studied the interactive effects of FSH and local ovarian factors, transforming growth factor beta1 (TGFbeta1) and androstenedione, on gelatinase secretion and progesterone production in rat ovarian granulosa cells. Granulosa cells of eCG-primed immature rats were treated once with various doses of FSH and TGFbeta1 and androstenedione alone or in combinations for 2 days. Conditioned media were analyzed for gelatinase activity using gelatin-zymography/densitometry and progesterone levels using enzyme immunoassay. Cell lysates were analyzed for steroidogenic acute regulatory (StAR) and cholesterol side-chain-cleavage (P450scc) enzyme protein levels. This study demonstrates for the first time that FSH dose-dependently increased the secretion of a major 63-kDa gelatinase and minor 92- and 67-kDa gelatinases. TGFbeta1 also dose-dependently increased the secretion of 63-kDa gelatinase, while androstenedione alone had no effect. The 92-kDa gelatinase was identified as the pro-MMP9 that could be cleaved by aminophenylmercuric acetate into the 83-kDa active form. Importantly, we show that TGFbeta1 and androgen act in an additive manner to enhance FSH stimulatory effects both on the secretion of gelatinases and the production of progesterone. We further show by immunoblotting that the enhancing effect of TGFbeta1 and androstenedione on FSH-stimulated steroidogenesis is partly mediated through the increased level of StAR protein and/or P450scc enzyme. In conclusion, this study indicates that, during antral follicle development, TGFbeta1 and androgen act to enhance FSH promotion of granulosa cell differentiation and that the process may involve the interplay of modulating cell- to-matrix/cell-to-cell interaction and steroidogenic activity.

  16. In utero-exposed di(n-butyl) phthalate induce dose dependent, age-related changes of morphology and testosterone-biosynthesis enzymes/associated proteins of Leydig cell mitochondria in rats.

    PubMed

    Motohashi, Masaya; Wempe, Michael F; Mutou, Tomoko; Okayama, Yuya; Kansaku, Norio; Takahashi, Hiroyuki; Ikegami, Masahiro; Asari, Masao; Wakui, Shin

    2016-04-01

    Female pregnant Sprague-Dawley rats were intragastrically (ig) administered di(n-butyl) phthalate (DBP) at four doses (0, 10, 50 and 100 mg/kg) during gestation days (GD) 12-21 (n = 5 per group). The age-related morphological changes of Leydig cell mitochondrion (LC-Mt) and testosterone biosynthesis enzymes/associated genes/proteins expression levels were investigated. As compared to the control (no DBP), the 10 mg, and 50 mg DBP dose groups, the 100 mg DBP dose group at weeks 5 and 7 showed a significant amount of small LC-Mt. Thereafter, from weeks 9 to 17, the LC-Mt size and quantity in the 100 mg DBP dose group increased and became statistically similar to the other dose groups; hence, dose and time-dependent LC-Mt changes were observed. Throughout the study, the 100 mg DBP dose group had significantly lower testosterone levels. In addition, the 100 mg DBP dose group displayed lower StAR (StAR, steroidogenic acute regulatory protein) and P450scc (CYP11a1, cholesterol side-chain cleavage enzyme) levels at weeks 5 and 7, but they became statistically similar to all other dose groups at weeks 9 to 17; in contrast, the SR-B1 (Sarb1, scavenger receptor class B member 1) levels were similar for all DBP dose groups. The rats in utero 100 mg DBP /kg/day (GD 12-21) exposure results from this study indicate a dose-dependent, age-related morphological change in LC-Mt which are linked to reductions in testosterone biosynthesis genes / proteins expression, specifically StAR and P450scc.

  17. Gene expression profiling during spermatogenesis in early maturing male Atlantic salmon parr testes.

    PubMed

    Maugars, Gersende; Schmitz, Monika

    2008-01-01

    The initiation of sexual maturation and spermatogenesis are complex processes that require the highly coordinated regulation of a number of key genes. The endocrine system plays crucial roles in these processes, but the precise mechanisms involved in sexual maturation of fish are poorly understood. We investigated the expression of genes encoding proteins involved in sex steroid biosynthesis (Ff1b (FTZ-F1 homolog), steroidogenic acute regulatory protein (StAR), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), 3beta-hydroxysteroid dehydrogenase/Delta(5)-Delta(4)-isomerase (3beta-HSD), cytochrome P450 17alpha-hydroxylase/17,20-lyase (P450c17), cytochrome P450 11beta-hydroxylase (P45011beta) and 11beta-hydroxysteroid dehydrogenase (11beta-HSD)) and the anti-Müllerian hormone (AMH) homolog during early sexual maturation of one-summer-old male Atlantic salmon parr by RT-PCR. Genes encoding Ff1b, StAR, 3beta-HSD, P450c17 and 11beta-HSD were upregulated during spermatogonial proliferation. During the course of spermatogenesis expression profiles of Ff1b, StAR, 3beta-HSD, P450scc, P450c17, P45011beta, and 11beta-HSD were similar; transcript levels being low during early stages, then strongly increasing during spermiogenesis. These results indicate that coordinated de novo transcription of genes encoding StAR as well as 3beta-HSD, P450c17 and 11beta-HSD might be required for sex steroids production during the initiation of spermatogenesis in salmon. In contrast, transcription levels of AMH were comparatively high in immature testes, decreased when spermatogenesis was initiated, and were lowest during spermiogenesis, suggesting that AMH suppression plays a crucial role in the process of spermatogenesis in salmonids. Correlation analyses show that FSH and LH might be differentially involved in the regulation of several of these genes studied.

  18. Exposure of preimplantation embryos to low-dose bisphenol A impairs testes development and suppresses histone acetylation of StAR promoter to reduce production of testosterone in mice.

    PubMed

    Hong, Juan; Chen, Fang; Wang, Xiaoli; Bai, Yinyang; Zhou, Rong; Li, Yingchun; Chen, Ling

    2016-05-15

    Previous studies have shown that bisphenol A (BPA) is a potential endocrine disruptor and testicular toxicant. The present study focused on exploring the impact of exposure to low dose of BPA on male reproductive development during the early embryo stage and the underlying mechanisms. BPA (20 μg/kg/day) was orally administered to female mice on days 1-5 of gestation. The male offspring were euthanized at PND10, 20, 24, 35 or PND50. We found that the mice exposed to BPA before implantation (BPA-mice) displayed retardation of testicular development with reduction of testosterone level. The diameter and epithelium height of seminiferous tubules were reduced in BPA-mice at PND35. The numbers of spermatogenic cells at different stages were significantly reduced in BPA-mice at PND50. BPA-mice showed a persistent reduction in serum and testicular testosterone levels starting from PND24, whereas GnRH mRNA was significantly increased at PND35 and PND50. The expressions of testicular StAR and P450scc in BPA-mice also decreased relative to those of the controls at PND35 and PND50. Further analysis found that the levels of histone H3 and H3K14 acetylation (Ac-H3 and H3K14ac) in the promoter of StAR were decreased relative to those of control mice, whereas the level of Ac-H3 in the promoter of P450scc was not significantly different between the groups. These results provide evidence that exposure to BPA in preimplantation embryo retards the development of testes by reducing histone acetylation of the StAR promoter to disrupt the testicular testosterone synthesis.

  19. Anti leukemia activity (Dunning ascitic) of 6-mercaptopurine and its metallo complexes in rats.

    PubMed

    Skinner, S M; Swatzell, J M; Lewis, R W

    1978-01-01

    Six-mercaptopurine and its Pt, Pd and Bi complexes were used at various dosage levels to treat Dunning ascitic leukemia in rats. Significant anticarcinogenic activity was found in all compounds with toxicity apparent at the highest dosage level of all but the platinum compound.

  20. Anti leukemia activity (L1210) of 6-mercaptopurine and its metallo complexes in mice.

    PubMed

    Skinner, S M; Lewis, R W

    1977-01-01

    Six-mercaptopurine in the free form and complexed with Pt, Pd, or Bi metals was used at various dosage levels to treat L1210 leukemia in mice. Anticarcinogenic activity was shown by six-mercaptopurine and the Pd and Bi complexes, inactivity by the Pt complex, and toxicity by the highest dosage level of six-mercaptopurine.

  1. The Physics of Equestrian Show Jumping

    ERIC Educational Resources Information Center

    Stinner, Art

    2014-01-01

    This article discusses the kinematics and dynamics of equestrian show jumping. For some time I have attended a series of show jumping events at Spruce Meadows, an international equestrian center near Calgary, Alberta, often referred to as the "Wimbledon of equestrian jumping." I have always had a desire to write an article such as this…

  2. Serving Up Activities for TV Cooking Shows.

    ERIC Educational Resources Information Center

    Katchen, Johanna E.

    This paper documents a presentation given on the use of English-language television cooking shows in English-as-a-Second-Language (ESL) and English-as-a-Foreign-Language (EFL) classrooms in Taiwan. Such shows can be ideal for classroom use, since they have a predictable structure consisting of short segments, are of interest to most students,…

  3. 47 CFR 90.505 - Showing required.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... MOBILE RADIO SERVICES Developmental Operation § 90.505 Showing required. (a) Except as provided in paragraph (b) of this section, each application for developmental operation shall be accompanied by a showing that: (1) The applicant has an organized plan of development leading to a specific objective;...

  4. The Language of Show Biz: A Dictionary.

    ERIC Educational Resources Information Center

    Sergel, Sherman Louis, Ed.

    This dictionary of the language of show biz provides the layman with definitions and essays on terms and expressions often used in show business. The overall pattern of selection was intended to be more rather than less inclusive, though radio, television, and film terms were deliberately omitted. Lengthy explanations are sometimes used to express…

  5. Gene Therapy Shows Promise for Aggressive Lymphoma

    MedlinePlus

    ... fullstory_163824.html Gene Therapy Shows Promise for Aggressive Lymphoma Over one-third of patients appeared disease- ... 2017 (HealthDay News) -- An experimental gene therapy for aggressive non-Hodgkin lymphoma beat back more than a ...

  6. Poverty Harder on Women's Hearts, Research Shows

    MedlinePlus

    ... page: https://medlineplus.gov/news/fullstory_163168.html Poverty Harder on Women's Hearts, Research Shows Poor females ... reduce the burden of cardiovascular disease around the world," Peters said. The study findings were published online ...

  7. Do dogs (Canis familiaris) show contagious yawning?

    PubMed

    Harr, Aimee L; Gilbert, Valerie R; Phillips, Kimberley A

    2009-11-01

    We report an experimental investigation into whether domesticated dogs display contagious yawning. Fifteen dogs were shown video clips of (1) humans and (2) dogs displaying yawns and open-mouth expressions (not yawns) to investigate whether dogs showed contagious yawning to either of these social stimuli. Only one dog performed significantly more yawns during or shortly after viewing yawning videos than to the open-mouth videos, and most of these yawns occurred to the human videos. No dogs showed significantly more yawning to the open-mouth videos (human or dog). The percentage of dogs showing contagious yawning was less than chimpanzees and humans showing this behavior, and considerably less than a recently published report investigating this behavior in dogs (Joly-Mascheroni et al. in Biol Lett 4:446-448, 2008).

  8. Spacecraft Image Mashup Shows Galactic Collision

    NASA Video Gallery

    This new composite image from the Chandra X-ray Observatory, the Hubble Space Telescope, and the Spitzer Space Telescope shows two colliding galaxies more than a 100 million years after they first ...

  9. Study Shows How Zika Attacks Infant Brain

    MedlinePlus

    ... gov/news/fullstory_162514.html Study Shows How Zika Attacks Infant Brain Virus can copy itself thousands ... New research paints a chilling portrait of how Zika ravages the infant brain. Scientists from the U.S. ...

  10. Fecal Transplant Shows Early Promise Against Autism

    MedlinePlus

    ... 163263.html Fecal Transplant Shows Early Promise Against Autism Small study found giving healthy gut bacteria to ... study suggests a novel treatment for kids with autism: Give these young patients a fresh supply of ...

  11. TRMM Satellite Shows Heavy Rainfall in Cristina

    NASA Video Gallery

    NASA's TRMM satellite rainfall data was overlaid on an enhanced visible/infrared image from NOAA's GOES-East satellite showing cloud and rainfall extent. Green areas indicate rainfall at over 20 mm...

  12. GOES Satellite Data Shows Tornado Development

    NASA Video Gallery

    This animation of NOAA's GOES-East satellite data shows the development and movement of the weather system that spawned tornadoes affecting the southern and eastern U.S. states on April 27-29, 2014...

  13. Educational Outreach: The Space Science Road Show

    NASA Astrophysics Data System (ADS)

    Cox, N. L. J.

    2002-01-01

    The poster presented will give an overview of a study towards a "Space Road Show". The topic of this show is space science. The target group is adolescents, aged 12 to 15, at Dutch high schools. The show and its accompanying experiments would be supported with suitable educational material. Science teachers at schools can decide for themselves if they want to use this material in advance, afterwards or not at all. The aims of this outreach effort are: to motivate students for space science and engineering, to help them understand the importance of (space) research, to give them a positive feeling about the possibilities offered by space and in the process give them useful knowledge on space basics. The show revolves around three main themes: applications, science and society. First the students will get some historical background on the importance of space/astronomy to civilization. Secondly they will learn more about novel uses of space. On the one hand they will learn of "Views on Earth" involving technologies like Remote Sensing (or Spying), Communication, Broadcasting, GPS and Telemedicine. On the other hand they will experience "Views on Space" illustrated by past, present and future space research missions, like the space exploration missions (Cassini/Huygens, Mars Express and Rosetta) and the astronomy missions (Soho and XMM). Meanwhile, the students will learn more about the technology of launchers and satellites needed to accomplish these space missions. Throughout the show and especially towards the end attention will be paid to the third theme "Why go to space"? Other reasons for people to get into space will be explored. An important question in this is the commercial (manned) exploration of space. Thus, the questions of benefit of space to society are integrated in the entire show. It raises some fundamental questions about the effects of space travel on our environment, poverty and other moral issues. The show attempts to connect scientific with

  14. Liquid Crystal Research Shows Deformation By Drying

    NASA Technical Reports Server (NTRS)

    2003-01-01

    These images, from David Weitz's liquid crystal research, show ordered uniform sized droplets (upper left) before they are dried from their solution. After the droplets are dried (upper right), they are viewed with crossed polarizers that show the deformation caused by drying, a process that orients the bipolar structure of the liquid crystal within the droplets. When an electric field is applied to the dried droplets (lower left), and then increased (lower right), the liquid crystal within the droplets switches its alignment, thereby reducing the amount of light that can be scattered by the droplets when a beam is shone through them.

  15. Show Them You Really Want the Job

    ERIC Educational Resources Information Center

    Perlmutter, David D.

    2012-01-01

    Showing that one really "wants" the job entails more than just really wanting the job. An interview is part Broadway casting call, part intellectual dating game, part personality test, and part, well, job interview. When there are 300 applicants for a position, many of them will "fit" the required (and even the preferred) skills listed in the job…

  16. EFFECT OF REPETITIVE FILM SHOWINGS ON LEARNING.

    ERIC Educational Resources Information Center

    MCTAVISH, C.L.; AND OTHERS

    A DETERMINATION OF THE INCREMENT IN LEARNING ATTRIBUTABLE SOLELY TO ONE, TWO, AND THREE REPETITIONS OF A FILM OVER AND ABOVE A SINGLE SHOWING WAS INVESTIGATED. THE SUBJECTS WERE 319 COLLEGE FRESHMEN WHO WERE DIVIDED INTO FOUR GROUPS AND WHO WERE SHOWN FOUR GENERAL SCIENCE FILMS. EACH GROUP SAW ONE OF THE FILMS ONCE, A SECOND FILM TWICE, A THIRD…

  17. Showing Enantiomorphous Crystals of Tartaric Acid

    ERIC Educational Resources Information Center

    Andrade-Gamboa, Julio

    2007-01-01

    Most of the articles and textbooks that show drawings of enantiomorphous crystals use an inadequate view to appreciate the fact that they are non-superimposable mirror images of one another. If a graphical presentation of crystal chirality is not evident, the main attribute of crystal enantiomorphism can not be recognized by students. The classic…

  18. Laser entertainment and light shows in education

    NASA Astrophysics Data System (ADS)

    Sabaratnam, Andrew T.; Symons, Charles

    2002-05-01

    Laser shows and beam effects have been a source of entertainment since its first public performance May 9, 1969, at Mills College in Oakland, California. Since 1997, the Photonics Center, NgeeAnn Polytechnic, Singapore, has been using laser shows as a teaching tool. Students are able to exhibit their creative skills and learn at the same time how lasers are used in the entertainment industry. Students will acquire a number of skills including handling three- phase power supply, operation of cooling system, and laser alignment. Students also acquire an appreciation of the arts, learning about shapes and contours as they develop graphics for the shows. After holography, laser show animation provides a combination of the arts and technology. This paper aims to briefly describe how a krypton-argon laser, galvanometer scanners, a polychromatic acousto-optic modulator and related electronics are put together to develop a laser projector. The paper also describes how students are trained to make their own laser animation and beam effects with music, and at the same time have an appreciation of the operation of a Class IV laser and the handling of optical components.

  19. 47 CFR 90.505 - Showing required.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES PRIVATE LAND MOBILE RADIO SERVICES Developmental Operation § 90.505 Showing required. (a) Except as provided in...) The actual transmission by radio is essential to proceed beyond the present stage of the program;...

  20. 47 CFR 90.505 - Showing required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES PRIVATE LAND MOBILE RADIO SERVICES Developmental Operation § 90.505 Showing required. (a) Except as provided in...) The actual transmission by radio is essential to proceed beyond the present stage of the program;...

  1. Tilapia show immunization response against Ich

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study compares the immune response of Nile tilapia and red tilapia against parasite Ichthyophthirius multifiliis (Ich) using a cohabitation challenge model. Both Nile and red tilapia showed strong immune response post immunization with live Ich theronts by IP injection or immersion. Blood serum...

  2. A Talk Show from the Past.

    ERIC Educational Resources Information Center

    Gallagher, Arlene F.

    1991-01-01

    Describes a two-day activity in which elementary students examine voting rights, the right to assemble, and women's suffrage. Explains the game, "Assemble, Reassemble," and a student-produced talk show with five students playing the roles of leaders of the women's suffrage movement. Profiles Elizabeth Cady Stanton, Lucretia Mott, Susan…

  3. State Data Show Gains in Reading

    ERIC Educational Resources Information Center

    Manzo, Kathleen Kennedy

    2007-01-01

    Schools taking part in the federal Reading First program are showing significant progress in boosting students' reading fluency and comprehension, according to state-reported data compiled and released by the U.S. Department of Education last week. In releasing for the first time detailed, multiyear data on how Reading First schools are performing…

  4. Human platelet sulfotransferase shows seasonal rhythms.

    PubMed

    Marazziti, D; Palego, L; Mazzanti, C; Silvestri, S; Cassano, G B

    1995-04-01

    Our study aimed to investigate the possible presence of seasonal changes in platelet phenolsulfotransferase (ST) in a group of 20 healthy, drug-free subjects of both sexes between 24 and 37 years of age. Blood samples were taken four times a year in the period immediately following the equinoxes and the solstices. The results showed that both Sts underwent seasonal changes: the lowest values were found in autumn and in winter, and the highest in the summer. A positive correlation between the two STs and the length of the photoperiod was observed in winter whereas in the spring we detected a negative correlation between the TL ST and the photoperiod length. Future studies should clarify whether platelet ST of patients with mood disorders shows a similar seasonality.

  5. Male genital leiomyomas showing androgen receptor expression.

    PubMed

    Suárez-Peñaranda, José Manuel; Vieites, Begoña; Evgenyeva, Elena; Vázquez-Veiga, Hugo; Forteza, Jeronimo

    2007-12-01

    Genital leiomyoma in men include those superficial leiomyomas arising in the scrotum and the areola. They are unusual neoplasms: few cases have been reported in the literature and they usually escape clinical diagnosis. Three cases of male genital leiomyomas are reported: two in the scrotum and one in the areola. They were all conservatively excised and the behaviour was completely benign in all cases. Histopathological examination showed the typical findings of superficial leiomyomas, with some minor differences between cases arising in the scrotum and those from the areola. Immunohistochemical findings not only confirmed the smooth muscle nature of all cases but also showed unequivocal immunostaining for androgen receptors in the leiomyomas from the scrotum. Immunostaining for androgen receptors in scrotal leiomyomas is, as far as we are aware, a previously unknown characteristic of male genital leiomyomas. This finding supports the role of steroid hormones in the growth of genital leiomyomas, similar to leiomyomas found in other locations.

  6. Kepler Systems That Show Multiple Transiting Objects

    NASA Astrophysics Data System (ADS)

    Steffen, Jason H.; Fabrycky, D. C.; Ford, E. B.; Holman, M. J.; Lissauer, J. J.; Ragozzine, D.; Welsh, W. F.; Kepler Science Team

    2011-01-01

    Exoplanetary systems that have multiple transiting planets provide unique and important insight into the formation, evolution, and dynamics of exoplanetary systems. Kepler has announced the discovery of a confirmed planetary system with multiple transiting planets (Kepler 9, Holman et al. 2010) as well as several candidate planetary systems that show multiple transiting objects (Steffen et al. 2010). Kepler 9 shows deviations from a constant period due to the ongoing dynamical interactions between the confirmed planets. From these transit timing variations (TTV) one can measure the planetary masses from the photometric data alone. The presence of several systems with multiple transiting candidates from the first quarter of data indicate that Kepler should continue to find systems with multiple transiting planets. Such systems will provide important, general information about the histories of planetary systems.

  7. Latest European coelacanth shows Gondwanan affinities.

    PubMed

    Cavin, Lionel; Forey, Peter L; Buffetaut, Eric; Tong, Haiyan

    2005-06-22

    The last European fossil occurrence of a coelacanth is from the Mid-Cretaceous of the English Chalk (Turonian, 90 million years ago). Here, we report the discovery of a coelacanth from Late Cretaceous non-marine rocks in southern France. It consists of a left angular bone showing structures that imply close phylogenetic affinities with some extinct Mawsoniidae. The closest relatives are otherwise known from Cretaceous continental deposits of southern continents and suggest that the dispersal of freshwater organisms from Africa to Europe occurred in the Late Cretaceous.

  8. Show Me the Invisible: Visualizing Hidden Content

    PubMed Central

    Geymayer, Thomas; Steinberger, Markus; Lex, Alexander; Streit, Marc; Schmalstieg, Dieter

    2014-01-01

    Content on computer screens is often inaccessible to users because it is hidden, e.g., occluded by other windows, outside the viewport, or overlooked. In search tasks, the efficient retrieval of sought content is important. Current software, however, only provides limited support to visualize hidden occurrences and rarely supports search synchronization crossing application boundaries. To remedy this situation, we introduce two novel visualization methods to guide users to hidden content. Our first method generates awareness for occluded or out-of-viewport content using see-through visualization. For content that is either outside the screen’s viewport or for data sources not opened at all, our second method shows off-screen indicators and an on-demand smart preview. To reduce the chances of overlooking content, we use visual links, i.e., visible edges, to connect the visible content or the visible representations of the hidden content. We show the validity of our methods in a user study, which demonstrates that our technique enables a faster localization of hidden content compared to traditional search functionality and thereby assists users in information retrieval tasks. PMID:25325078

  9. Surveys show support for green 'activities'.

    PubMed

    Baillie, Jonathan

    2012-03-01

    Two independently conducted surveys on sustainability - one into the 'views and values' of NHS 'leaders', and the other questioning the public about the importance of the 'green agenda' in the NHS, and their opinions on how the service might most effectively reduce its carbon footprint, form the basis of Sustainability in the NHS: Health Check 2012, a new NHS Sustainable Development Unit (NHS SDU) publication. As HEJ editor Jonathan Baillie reports, the new document also presents updated data on the 'size' of the carbon footprint of the NHS in England, showing that, although good work by a number of Trusts in the past two years has seen healthcare-generated carbon emissions start to 'level off', the biggest contributors have been the current health service spending review, and the increased national availability of renewable energy.

  10. Star Shows It Has The Right Stuff

    NASA Astrophysics Data System (ADS)

    2004-01-01

    Astronomers have used an observation by NASA's Chandra X-ray Observatory to make the best case yet that a star can be engulfed by its companion star and survive. This discovery will help astronomers better understand how closely coupled stars, and perhaps even stars and planets, evolve when one of the stars expands enormously in its red giant phase. The binary star system known as V471 Tauri comprises a white dwarf star (the primary) in a close orbit -- one thirtieth of the distance between Mercury and the Sun -- with a normal Sun-like star (the secondary). Chandra's data showed that the hot upper atmosphere of the secondary star has a deficit of carbon atoms relative to nitrogen atoms. "This deficit of carbon atoms is the first clear observational evidence that the normal star was engulfed by its companion in the past," according to Jeremy Drake of the Smithsonian Astrophysical Observatory in Cambridge, MA, who coauthored an article on V471 in The Astrophysical Journal Letters with Marek Sarna of the N. Copernicus Astronomical Center in Poland. The white dwarf star was once a star several times as massive as the Sun. Nuclear fusion reactions in the core of such a star convert carbon into nitrogen over a period of about a billion years. When the fuel in the core of the star is exhausted, the core collapses, triggering more energetic nuclear reactions that cause the star to expand and transform into a red giant before eventually collapsing to become a white dwarf. The carbon-poor material in the core of the red giant is mixed with outer part of the star, so its atmosphere shows a deficit of carbon, as compared with Sun-like stars. The X-ray spectra of a red giant star (top panel) and a Sun-like star (bottom panel) show the large difference in the peaks due to carbon atoms in the two stars. Theoretical calculations indicate that a red giant in a binary system can completely envelop its companion star and dramatically affect its evolution. During this common envelope

  11. Microbiological and environmental issues in show caves.

    PubMed

    Saiz-Jimenez, Cesareo

    2012-07-01

    Cultural tourism expanded in the last half of the twentieth century, and the interest of visitors has come to include caves containing archaeological remains. Some show caves attracted mass tourism, and economical interests prevailed over conservation, which led to a deterioration of the subterranean environment and the rock art. The presence and the role of microorganisms in caves is a topic that is often ignored in cave management. Knowledge of the colonisation patterns, the dispersion mechanisms, and the effect on human health and, when present, over rock art paintings of these microorganisms is of the utmost importance. In this review the most recent advances in the study of microorganisms in caves are presented, together with the environmental implications of the findings.

  12. Mesenchymal stem cells show radioresistance in vivo.

    PubMed

    Singh, Sarvpreet; Kloss, Frank R; Brunauer, Regina; Schimke, Magdalena; Jamnig, Angelika; Greiderer-Kleinlercher, Brigitte; Klima, Günter; Rentenberger, Julia; Auberger, Thomas; Hächl, Oliver; Rasse, Michael; Gassner, Robert; Lepperdinger, Günter

    2012-04-01

    Irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (MSC), which play a pivotal role in bone regeneration. As a consequence of radiotherapy, bones may develop osteoradionecrosis. When irradiating human bone-derived MSC in vitro with increasing doses, the cells' self-renewal capabilities were greatly reduced. Mitotically stalled cells were still capable of differentiating into osteoblasts and pre-adipocytes. As a large animal model comparable to the clinical situation, pig mandibles were subjected to fractionized radiation of 2 χ 9 Gy within 1 week. This treatment mimics that of a standardized clinical treatment regimen of head and neck cancer patients irradiated 30 χ 2 Gy. In the pig model, fractures which had been irradiated, showed delayed osseous healing. When isolating MSC at different time points post-irradiation, no significant changes regarding proliferation capacity and osteogenic differentiation potential became apparent. Therefore, pig mandibles were irradiated with a single dose of either 9 or 18 Gy in vivo, and MSC were isolated immediately afterwards. No significant differences between the untreated and 9 Gy irradiated bone with respect to proliferation and osteogenic differentiation were unveiled. Yet, cells isolated from 18 Gy irradiated specimens exhibited a reduced osteogenic differentiation capacity, and during the first 2 weeks proliferation rates were greatly diminished. Thereafter, cells recovered and showed normal proliferation behaviour. These findings imply that MSC can effectively cope with irradiation up to high doses in vivo. This finding should thus be implemented in future therapeutic concepts to protect regenerating tissue from radiation consequences.

  13. VLA Shows "Boiling" in Atmosphere of Betelgeuse

    NASA Astrophysics Data System (ADS)

    1998-04-01

    A team of astronomers says that observations with the National Science Foundation's Very Large Array (VLA) radio telescope show that a neighboring bloated star has giant convective plumes propelling gas from its surface (photosphere) up into the star's atmosphere. This new information contradicts long-held ideas that such stellar atmospheres are more uniform, and may resolve questions about how the star's atmosphere attains its enormous size as well as how dust and gas is driven away from the star. Jeremy Lim of the Academia Sinica Institute of Astronomy & Astrophysics in Taiwan; Chris Carilli, Anthony Beasley, and Ralph Marson of the National Radio Astronomy Observatory (NRAO) in Socorro, NM; and Stephen White of the University of Maryland studied the red-supergiant star Betelgeuse, about 430 light-years away in the constellation Orion. They reported their findings in the April 9 issue of the scientific journal Nature. "These radio-telescope images confirm that Betelgeuse -- already more than 600 times larger than our Sun -- has a dense atmosphere that extends to many times larger still than the star itself," said Lim. "The highest-resolution image shows the star's atmosphere to have a remarkably complex structure." "To our surprise," added White, "the images also show that most of the gas in the atmosphere is only about as hot as that on the surface. Previously, all of it was thought to be very much hotter." The astronomers used the VLA to make images of Betelgeuse at a variety of radio frequencies. The series of radio observations measured the temperature of the star's atmosphere at different heights. Previous observations with the Hubble Space Telescope (HST) at ultraviolet wavelengths showed that the star's atmosphere contains very hot gas at about twice the surface temperature. The VLA images showed that there also is lower-temperature gas throughout the atmosphere. This gas is near the surface temperature at low heights and decreases in temperature

  14. The earliest published electrocardiogram showing ventricular preexcitation.

    PubMed

    Von Knorre, Georg H

    2005-03-01

    When in 1930, Wolff, Parkinson, and White published what is today known as the WPW, or preexcitation syndrome, they, and subsequently others, found few comparable cases in the preceding literature. Among these the report of Cohn and Fraser, published in 1913, was the earliest. However, another even earlier documentation in a 1909 article by Hoffmann escaped notice till now. The ECG of a patient with paroxysmal tachycardia reveals a short PR interval and a delta-wave-induced widening of the QRS complex, even though the reproduced tachycardia was not preexcitation related. The interpretation of this poorly reproduced ECG can be confirmed by another and more detailed description of the patient in an electrocardiography textbook published in 1914 by the same author. Thus, the earliest publication of an ECG showing ventricular preexcitation now can be dated back to 1909. Moreover, the Hoffmann monograph contains two additional examples of the WPW syndrome not noticed until now. All three cases published by Hoffmann had their first ECG recordings in 1912 or earlier.

  15. NASA GIBS Use in Live Planetarium Shows

    NASA Astrophysics Data System (ADS)

    Emmart, C. B.

    2015-12-01

    The American Museum of Natural History's Hayden Planetarium was rebuilt in year 2000 as an immersive theater for scientific data visualization to show the universe in context to our planet. Specific astrophysical movie productions provide the main daily programming, but interactive control software, developed at AMNH allows immersive presentation within a data aggregation of astronomical catalogs called the Digital Universe 3D Atlas. Since 2006, WMS globe browsing capabilities have been built into a software development collaboration with Sweden's Linkoping University (LiU). The resulting Uniview software, now a product of the company SCISS, is operated by about fifty planetariums around that world with ability to network amongst the sites for global presentations. Public presentation of NASA GIBS has allowed authoritative narratives to be presented within the range of data available in context to other sources such as Science on a Sphere, NASA Earth Observatory and Google Earth KML resources. Specifically, the NOAA supported World Views Network conducted a series of presentations across the US that focused on local ecological issues that could then be expanded in the course of presentation to national and global scales of examination. NASA support of for GIBS resources in an easy access multi scale streaming format like WMS has tremendously enabled particularly facile presentations of global monitoring like never before. Global networking of theaters for distributed presentations broadens out the potential for impact of this medium. Archiving and refinement of these presentations has already begun to inform new types of documentary productions that examine pertinent, global interdependency topics.

  16. Tetrahydrobiopterin shows chaperone activity for tyrosine hydroxylase.

    PubMed

    Thöny, Beat; Calvo, Ana C; Scherer, Tanja; Svebak, Randi M; Haavik, Jan; Blau, Nenad; Martinez, Aurora

    2008-07-01

    Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of catecholamine neurotransmitters. Primary inherited defects in TH have been associated with l-DOPA responsive and non-responsive dystonia and infantile parkinsonism. In this study, we show that both the cofactor (6R)-l-erythro-5,6,7,8-tetrahydrobiopterin (BH(4)) and the feedback inhibitor and catecholamine product dopamine increase the kinetic stability of human TH isoform 1 in vitro. Activity measurements and synthesis of the enzyme by in vitro transcription-translation revealed a complex regulation by the cofactor including both enzyme inactivation and conformational stabilization. Oral BH(4) supplementation to mice increased TH activity and protein levels in brain extracts, while the Th-mRNA level was not affected. All together our results indicate that the molecular mechanisms for the stabilization are a primary folding-aid effect of BH(4) and a secondary effect by increased synthesis and binding of catecholamine ligands. Our results also establish that orally administered BH(4) crosses the blood-brain barrier and therapeutic regimes based on BH(4) supplementation should thus consider the effect on TH. Furthermore, BH(4) supplementation arises as a putative therapeutic agent in the treatment of brain disorders associated with TH misfolding, such as for the human TH isoform 1 mutation L205P.

  17. Temperature Data Shows Warming in 2001

    NASA Technical Reports Server (NTRS)

    2002-01-01

    TThe figure above depicts how much air temperatures near the Earth's surface changed relative to the global mean temperature from 1951 to 1980. NASA researchers used maps of urban areas derived from city lights data to account for the 'heat island' effect of cities. The red and orange colors show that temperatures are warmer in most regions of the world when compared to the 1951 to 1980 'normal' temperatures. Warming around the world has been widespread, but it is not present everywhere. The largest warming is in Northern Canada, Alaska and Siberia, as indicated by the deeper red colors. The lower 48 United States have become warmer recently, but only enough to make the temperatures comparable to what they were in the 1930s. The scale on the bottom of these temperature anomaly images represent degrees in Celsius. The negative numbers represent cooling and the positive numbers depict warming. Overall, the air temperature near the Earth's surface has warmed by 1oF (0.6oC) globally, on average, over the last century. For more information and additional images, read Satellites Shed Light on a Warmer World. Image courtesy Goddard Institute for Space Studies (GISS).

  18. First K2 mutiplanatary system showing TTVs

    NASA Astrophysics Data System (ADS)

    Barros, Susana C. C.

    2015-08-01

    In traditional transit timing variations (TTVs) analysis of multi-planetary systems, the individual TTVs are first derived from transit fitting and later modeled using n-body dynamic simulations to constrain planetary masses.I will show that fitting simultaneously the transit light curves with the system dynamics (photo-dynamical model) increases the precision of the TTV measurements and helps constrain the system architecture. I will exemplify the advantages of applying this photo-dynamical model to a multi-planetary system found in K2 data very close to 3:2 mean motion resonance. In this case the period of the larger TTV variations (libration period) is much longer (~1.5 years) than the duration of the K2 observations (~80 days). However, our method allows to detect the short period TTVs produced by the orbital conjunctions between the planets that in turn permits to uniquely characterize the system. Therefore, our method can be used to constrain the masses of near-resonant systems even when the full libration curve is not observed and hence will help understanding evolution of these interesting systems.

  19. Ancient bacteria show evidence of DNA repair

    PubMed Central

    Johnson, Sarah Stewart; Hebsgaard, Martin B.; Christensen, Torben R.; Mastepanov, Mikhail; Nielsen, Rasmus; Munch, Kasper; Brand, Tina; Gilbert, M. Thomas P.; Zuber, Maria T.; Bunce, Michael; Rønn, Regin; Gilichinsky, David; Froese, Duane; Willerslev, Eske

    2007-01-01

    Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability. PMID:17728401

  20. AD-1 multiple exposure showing wing sweep

    NASA Technical Reports Server (NTRS)

    1980-01-01

    This photograph is a multiple exposure showing the AD-1 aircraft with its wing swept at different angles between zero and 60 degrees. The Ames-Dryden-1 (AD-1) aircraft was designed to investigate the concept of an oblique (pivoting) wing. The wing could be rotated on its center pivot, so that it could be set at its most efficient angle for the speed at which the aircraft was flying. NASA Ames Research Center Aeronautical Engineer Robert T. Jones conceived the idea of an oblique wing. His wind tunnel studies at Ames (Moffett Field, CA) indicated that an oblique wing design on a supersonic transport might achieve twice the fuel economy of an aircraft with conventional wings. The oblique wing on the AD-1 pivoted about the fuselage, remaining perpendicular to it during slow flight and rotating to angles of up to 60 degrees as aircraft speed increased. Analytical and wind tunnel studiesthat Jones conducted at Ames indicated that a transport-sized oblique-wing aircraft flying at speeds of up to Mach 1.4 (1.4 times the speed of sound) would have substantially better aerodynamic performance than aircraft with conventional wings. The AD-1 structure allowed the project to complete all of its technical objectives. The type of low-speed, low-cost vehicle - as expected - exhibited aeroelastic and pitch-roll-coupling effects that contributed to poor handling at sweep angles above 45 degrees. The fiberglass structure limited the wing stiffness that would have improved the handling qualities. Thus, after completion of the AD-1 project, there was still a need for a transonic oblique-wing research aircraft to assess the effects of compressibility, evaluate a more representative structure, and analyze flight performance at transonic speeds (those on either side of the speed of sound). The aircraft was delivered to the Dryden Flight Research Center, Edwards, CA, in March 1979 and its first flight was on December 21, 1979. Piloting the aircraft on that flight, as well as on its last

  1. Mercury's Core Molten, Radar Study Shows

    NASA Astrophysics Data System (ADS)

    2007-05-01

    100 times, and showed that Mercury's spin axis is almost, but not exactly, perpendicular to the plane of its rotation around the Sun," Margot said. Margot worked with Stanton Peale of the University of California, Santa Barbara, Raymond Jurgens and Martin Slade of NASA's Jet Propulsion Laboratory, and Igor Holin of the Space Research Institute in Moscow. The National Radio Astronomy Observatory is a facility of the National Science Foundation, operated under cooperative agreement by Associated Universities, Inc. The Arecibo Observatory is part of the National Astronomy and Ionosphere Center, which is operated by Cornell University under a cooperative agreement with the NSF. Part of this work was supported by the Jet Propulsion Laboratory, operated by Caltech under contract with NASA.

  2. Cold-induced changes in stress hormone and steroidogenic transcript levels in cunner (Tautogolabrus adspersus), a fish capable of metabolic depression.

    PubMed

    Alzaid, Abdullah; Hori, Tiago S; Hall, Jennifer R; Rise, Matthew L; Gamperl, A Kurt

    2015-12-01

    The cunner (Tautogolabrus adspersus) is a fish with a wide latitudinal distribution that is capable of going into metabolic depression during the winter months, and thus, represents a unique model to investigate the impacts of cold temperatures on the stress response. In this study, we measured resting (pre-stress) plasma cortisol levels in 10 °C and 0 °C acclimated cunner from Newfoundland, and both catecholamine and cortisol levels after they were given a standardized handling stress (i.e. 1 min air exposure). In addition, we cloned and characterized cDNAs for several key genes of the cortisol-axis [cytochrome P450scc, steroidogenic acute regulatory protein (StAR) and a glucocorticoid receptor (GR) most likely to be an ortholog of the teleost GR2], determined the tissue distribution of their transcripts, and measured their constitutive (i.e. pre-stress) transcript levels in individuals acclimated to both temperatures. In cunner acclimated to 0 °C, post-stress epinephrine and norepinephrine levels were much lower (by approximately 9- and 5-fold, respectively) compared to 10 °C acclimated fish, and these fish had relatively low resting cortisol levels (~15 ngml(-1)) and showed a typical post-stress response. In contrast, those acclimated to 10 °C had quite high resting cortisol levels (~75 ngml(-1)) that actually decreased (to ~20 ngml(-1)) post-stress before returning to pre-stress levels. Finally, fish acclimated to 10 °C had higher P450scc transcript levels in the head kidney and lower levels of GR transcript in both the head kidney and liver. Taken together, these results suggest that: (1) temperature has a profound effect on the stress response of this species; and (2) although the ancestors of this species inhabited warm waters (i.e. they are members of the family Labridae), populations of cunner from colder regions may show signs of stress at temperatures as low as 10 °C.

  3. Anti-leukemia effects of the novel synthetic 1-benzylindole derivative 21-900 in vitro and in vivo

    PubMed Central

    HuangFu, Wei-Chun; Chao, Min-Wu; Cheng, Chun-Chun; Wei, Yu-Chieh; Wu, Yi-Wen; Liou, Jing-Ping; Hsiao, George; Lee, Yu-Ching; Yang, Chia-Ron

    2017-01-01

    Cancers are the major cause of death worldwide. Chemotherapy using cytotoxic drugs and targeted therapy is required when surgery is difficult, ineffective, or impossible. We previously synthesized the novel synthetic 1-benzylindole derivative 21-900 and found that it inhibits histone deacetylase (HDAC) activities and tubulin assembly. Here we tested its effects on the human leukaemia cell lines HL-60 and MOLT-4 in vitro and in vivo. We found that its potent cytotoxic effects were mediated through cell cycle arrest at the G2/M phase, which increased the population of sub-G1 cells, leading to apoptosis. Further, tubulin was depolymerized by 21-900 in a manner similar to that of vincristine, leading to disruption of microtubule dynamics and increased levels of the mitotic marker MPM-2. Further, 21-900 increased the expression of cleavage form of poly (ADP-ribose) polymerase (PARP), caspase 3, 7 (cleavage form), and pro-apoptotic protein BAK and decreased the expression of pro-survival BCL-2-family proteins BCL-2, MCL-1, and BID pro-form, leading to the induction of apoptosis. The growth of tumours in nude mice formed by xenografts of HL-60 and MOLT-4 cells was significantly inhibited by 21-900 without causing the mice to lose body weight. These findings indicate that 21-900 may serve as a potent anti-leukaemia drug. PMID:28181578

  4. Anti-Leukemia Activity of In Vitro-Expanded Human Gamma Delta T Cells in a Xenogeneic Ph+ Leukemia Model

    PubMed Central

    Siegers, Gabrielle M.; Felizardo, Tania C.; Mathieson, A. Mark; Kosaka, Yoko; Wang, Xing-Hua; Medin, Jeffrey A.; Keating, Armand

    2011-01-01

    Gamma delta T cells (GDTc) lyse a variety of hematological and solid tumour cells in vitro and in vivo, and are thus promising candidates for cellular immunotherapy. We have developed a protocol to expand human GDTc in vitro, yielding highly cytotoxic Vgamma9/Vdelta2 CD27/CD45RA double negative effector memory cells. These cells express CD16, CD45RO, CD56, CD95 and NKG2D. Flow cytometric, clonogenic, and chromium release assays confirmed their specific cytotoxicity against Ph+ cell lines in vitro. We have generated a fluorescent and bioluminescent Ph+ cell line, EM-2eGFPluc, and established a novel xenogeneic leukemia model. Intravenous injection of EM-2eGFPluc into NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice resulted in significant dose-dependent bone marrow engraftment; lower levels engrafted in blood, lung, liver and spleen. In vitro-expanded human GDTc injected intraperitoneally were found at higher levels in blood and organs compared to those injected intravenously; GDTc survived at least 33 days post-injection. In therapy experiments, we documented decreased bone marrow leukemia burden in mice treated with GDTc. Live GDTc were found in spleen and bone marrow at endpoint, suggesting the potential usefulness of this therapy. PMID:21304898

  5. Showing and Telling Farming: Agricultural Shows and Re-Imaging British Agriculture

    ERIC Educational Resources Information Center

    Holloway, Lewis

    2004-01-01

    Some actors in the ''mainstream'' agricultural sector are beginning to engage in strategies of influencing public perceptions of farming, responding to public anxieties over industrialised agriculture and to a supposed separation of non-farming publics from food production. This paper focuses on agricultural shows as sites and events central to…

  6. Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity.

    PubMed

    Mohan, Vijee; Gupta, Soni; Thomas, Sherinmol; Mickey, Hanjabam; Charakana, Chaitanya; Chauhan, Vineeta Singh; Sharma, Kapil; Kumar, Rakesh; Tyagi, Kamal; Sarma, Supriya; Gupta, Suresh Kumar; Kilambi, Himabindu Vasuki; Nongmaithem, Sapana; Kumari, Alka; Gupta, Prateek; Sreelakshmi, Yellamaraju; Sharma, Rameshwar

    2016-01-01

    Domestication of tomato has resulted in large diversity in fruit phenotypes. An intensive phenotyping of 127 tomato accessions from 20 countries revealed extensive morphological diversity in fruit traits. The diversity in fruit traits clustered the accessions into nine classes and identified certain promising lines having desirable traits pertaining to total soluble salts (TSS), carotenoids, ripening index, weight and shape. Factor analysis of the morphometric data from Tomato Analyzer showed that the fruit shape is a complex trait shared by several factors. The 100% variance between round and flat fruit shapes was explained by one discriminant function having a canonical correlation of 0.874 by stepwise discriminant analysis. A set of 10 genes (ACS2, COP1, CYC-B, RIN, MSH2, NAC-NOR, PHOT1, PHYA, PHYB and PSY1) involved in various plant developmental processes were screened for SNP polymorphism by EcoTILLING. The genetic diversity in these genes revealed a total of 36 non-synonymous and 18 synonymous changes leading to the identification of 28 haplotypes. The average frequency of polymorphism across the genes was 0.038/Kb. Significant negative Tajima'D statistic in two of the genes, ACS2 and PHOT1 indicated the presence of rare alleles in low frequency. Our study indicates that while there is low polymorphic diversity in the genes regulating plant development, the population shows wider phenotype diversity. Nonetheless, morphological and genetic diversity of the present collection can be further exploited as potential resources in future.

  7. Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity

    PubMed Central

    Mohan, Vijee; Gupta, Soni; Thomas, Sherinmol; Mickey, Hanjabam; Charakana, Chaitanya; Chauhan, Vineeta Singh; Sharma, Kapil; Kumar, Rakesh; Tyagi, Kamal; Sarma, Supriya; Gupta, Suresh Kumar; Kilambi, Himabindu Vasuki; Nongmaithem, Sapana; Kumari, Alka; Gupta, Prateek; Sreelakshmi, Yellamaraju; Sharma, Rameshwar

    2016-01-01

    Domestication of tomato has resulted in large diversity in fruit phenotypes. An intensive phenotyping of 127 tomato accessions from 20 countries revealed extensive morphological diversity in fruit traits. The diversity in fruit traits clustered the accessions into nine classes and identified certain promising lines having desirable traits pertaining to total soluble salts (TSS), carotenoids, ripening index, weight and shape. Factor analysis of the morphometric data from Tomato Analyzer showed that the fruit shape is a complex trait shared by several factors. The 100% variance between round and flat fruit shapes was explained by one discriminant function having a canonical correlation of 0.874 by stepwise discriminant analysis. A set of 10 genes (ACS2, COP1, CYC-B, RIN, MSH2, NAC-NOR, PHOT1, PHYA, PHYB and PSY1) involved in various plant developmental processes were screened for SNP polymorphism by EcoTILLING. The genetic diversity in these genes revealed a total of 36 non-synonymous and 18 synonymous changes leading to the identification of 28 haplotypes. The average frequency of polymorphism across the genes was 0.038/Kb. Significant negative Tajima’D statistic in two of the genes, ACS2 and PHOT1 indicated the presence of rare alleles in low frequency. Our study indicates that while there is low polymorphic diversity in the genes regulating plant development, the population shows wider phenotype diversity. Nonetheless, morphological and genetic diversity of the present collection can be further exploited as potential resources in future. PMID:27077652

  8. Dehydroepiandrosterone Biosynthesis, Role, and Mechanism of Action in the Developing Neural Tube

    PubMed Central

    Galdo, Mark; Gregonis, Jennifer; Fiore, Christelle S.; Compagnone, Nathalie A.

    2011-01-01

    Dehydroepiandrosterone (DHEA) is synthesized from cholesterol by activity of P450scc and P450c17, enzymes that we previously characterized in the developing nervous system. We describe the localization of P450c17 in the differentiated field of the ventral spinal cord in different motor neuron subtypes. We show that, during organogenesis, P450c17 activity is regulated along the antero/posterior axis of the spinal cord concomitantly with the gradient of neurogenesis. To examine whether DHEA may modulate this process, we measured proliferation and differentiation of ventral neural precursors in primary and explant cultures. Our results showed that DHEA-induced the expression of class II protein Nkx6.1, motor neuron precursor Olig-2, and definitive motor neuron marker Isl-1/2. DHEA also promoted proliferation of ventrally committed precursors in isolated spinal cord precursor cultures and in whole spinal cord explants. Both the proliferative and inductive effects of DHEA were dependent on sonic hedgehog signaling. The possibilities that the effects observed with DHEA were due to its metabolism into androgens or to activation of NMDA receptors were excluded. These results support the hypothesis that the tight regulation of DHEA biosynthesis may be a biologic clock restricting the period of ventral neuronal-precursor proliferation, thus controlling the number of pre-committed neurons in the developing neural tube. PMID:22649409

  9. Transcriptional regulation of steroid hydroxylase genes by corticotropin.

    PubMed Central

    John, M E; John, M C; Boggaram, V; Simpson, E R; Waterman, M R

    1986-01-01

    Maintenance of optimal steroidogenic capacity in the adrenal cortex is the result of a cAMP-dependent response to the peptide hormone corticotropin (ACTH). The molecular mechanism of this action of ACTH has been examined by using five recombinant DNA clones specific for enzymes of the steroidogenic pathway (P-450scc, P-45011 beta, P-450C21, P-45017 alpha, and adrenodoxin). The presence of nuclear precursors in steady-state RNA samples derived from cultured bovine adrenocortical cells and moderate increases in the number of RNA chain initiations, as determined by in vitro nuclear run-off assays, indicate that ACTH controls the expression of the gene(s) for each of these proteins at the transcriptional level. The ACTH-mediated increase in accumulation of transcripts specific for steroid hydroxylases in nuclear RNA can be specifically blocked by inhibiting protein synthesis in bovine adrenocortical cell cultures. The steady-state concentrations of nuclear RNA for control genes show no decrease upon cycloheximide treatment. These studies suggest that a primary action of ACTH in the adrenal cortex is to activate (via cAMP) the synthesis of rapidly turning over protein factors that in turn mediate increased initiation of transcription of steroid hydroxylase genes. We propose that these protein factors impart specificity of induction to genes encoding components of this pathway in steroidogenic tissues. Images PMID:3014507

  10. Design, Synthesis and Biological Activities of Novel Gemini 20S-Hydroxyvitamin D3 Analogs.

    PubMed

    Lin, Zongtao; Marepally, Srinivasa R; Kim, Tae-Kang; Janjetovic, Zorica; Oak, Allen Sw; Postlethwaite, Arnold E; Myers, Linda K; Tuckey, Robert C; Slominski, Andrzej T; Miller, Duane D; Li, Wei

    2016-03-01

    Vitamin D3 (D3) can be metabolized by cytochrome P450scc (CYP11A1) into 20S-hydroxyvitamin D3 (20D3) as a major metabolite. This bioactive metabolite has shown strong antiproliferative, antifibrotic, pro-differentiation and anti-inflammatory effects while being non-toxic (non-calcemic) at high concentrations. Since D3 analogs with two symmetric side chains (Gemini analogs) result in potent activation of the vitamin D receptor (VDR), we hypothesized that the chain length and composition of these types of analogs also containing a 20-hydroxyl group would affect their biological activities. In this study, we designed and synthesized a series of Gemini 20D3 analogs. Biological tests showed that some of these analogs are partial VDR activators and can significantly stimulate the expression of mRNA for VDR and VDR-regulated genes including CYP24A1 and transient receptor potential cation channel V6 (TRPV6). These analogs inhibited the proliferation of melanoma cells with potency comparable to that of 1α,25-dihydroxyvitamin D3. Moreover, these analogs reduced the level of interferon γ and up-regulated the expression of leukocyte associated immunoglobulin-like receptor 1 in splenocytes, indicating that they have potent anti-inflammatory activities. There are no clear correlations between the Gemini chain length and their VDR activation or biological activities, consistent with the high flexibility of the ligand-binding pocket of the VDR.

  11. Design, Synthesis and Biological Activities of Novel Gemini 20S-Hydroxyvitamin D3 Analogs

    PubMed Central

    LIN, ZONGTAO; MAREPALLY, SRINIVASA R.; KIM, TAE-KANG; JANJETOVIC, ZORICA; OAK, ALLEN SW.; POSTLETHWAITE, ARNOLD E.; MYERS, LINDA K.; TUCKEY, ROBERT C.; SLOMINSKI, ANDRZEJ T.; MILLER, DUANE D.; LI, WEI

    2017-01-01

    Vitamin D3 (D3) can be metabolized by cytochrome P450scc (CYP11A1) into 20S-hydroxyvitamin D3 (20D3) as a major metabolite. This bioactive metabolite has shown strong antiproliferative, antifibrotic, pro-differentiation and anti-inflammatory effects while being non-toxic (non-calcemic) at high concentrations. Since D3 analogs with two symmetric side chains (Gemini analogs) result in potent activation of the vitamin D receptor (VDR), we hypothesized that the chain length and composition of these types of analogs also containing a 20-hydroxyl group would affect their biological activities. In this study, we designed and synthesized a series of Gemini 20D3 analogs. Biological tests showed that some of these analogs are partial VDR activators and can significantly stimulate the expression of mRNA for VDR and VDR-regulated genes including CYP24A1 and transient receptor potential cation channel V6 (TRPV6). These analogs inhibited the proliferation of melanoma cells with potency comparable to that of 1α,25-dihydroxyvitamin D3. Moreover, these analogs reduced the level of interferon γ and up-regulated the expression of leukocyte associated immunoglobulin-like receptor 1 in splenocytes, indicating that they have potent anti-inflammatory activities. There are no clear correlations between the Gemini chain length and their VDR activation or biological activities, consistent with the high flexibility of the ligand-binding pocket of the VDR. PMID:26976974

  12. ALPK1 affects testosterone mediated regulation of proinflammatory cytokines production.

    PubMed

    Kuo, Tzer-Min; Yeh, Kun-Tu; Hsu, Hui-Ting; Chiang, Shang-Lun; Chang, Jan-Gowth; Huang, Chung-Ming; Tu, Hung-Pin; Liu, Chiu-Shong; Ko, Ying-Chin

    2015-11-01

    Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). In addition to having an inductive effect on the proinflammatory cytokines in monocytic THP1 cells, ALPK1 is expressed abundantly in the mouse testes. Low testosterone levels are commonly associated with arthritis, CKD, type 2 DM, cardiovascular disease and inflammation. The testosterone's anti-inflammatory effect has been demonstrated to reduce proinflammatory cytokines and adhesion molecules. In this study, we found that ALPK1 transgenic mice showed lower levels of testosterone in both the testes and the serum. Decreasing endogenous ALPK1 enhanced testosterone levels and transcripts of testosterone-regulated genes (P450scc, 3beta-HSD, P450C17, 17beta-HSD, StAR, and INSL3) in TM3 Leydig cells. In contrast, increasing testosterone decreased ALPK1 in both TM3 and monocytic THP1 cells. This decrease was accompanied by a reduction of the proinflammatory cytokines. Increased ALPK1 levels attenuated the testosterone effects in THP1 cells. Finally, we also found that ALPK1 increased the release of TNF-alpha and TGF-beta1 in the human embryonic kidney 293 cells, while testosterone inhibited ALPK1 in the primary kidney cells. Taken together, this data suggests that the balance between ALPK1 and testosterone plays a critical role in the testosterone-mediated inhibition of proinflammatory cytokines.

  13. Protective Effect of Selenium on Aflatoxin B1-Induced Testicular Toxicity in Mice.

    PubMed

    Cao, Zheng; Shao, Bing; Xu, Feibo; Liu, Yunfeng; Li, Yanfei; Zhu, Yanzhu

    2017-03-27

    Aflatoxins have been considered as one of the major risk factors of male infertility, and aflatoxin B1 (AFB1) is the most highly toxic and prevalent member of the aflatoxins family. Selenium (Se), an essential nutritional trace mineral for normal testicular development and male fertility, has received extensive intensive on protective effects of male reproductive system due to its potential antioxidant and activating testosterone synthesis. To investigate the protective effect of Se on AFB1-induced testicular toxicity, the mice were orally administered with AFB1 (0.75 mg/kg) and Se (0.2 mg/kg or 0.4 mg/kg) for 45 days. We found that that Se elevated testes index, sperm functional parameters (concentration, malformation, and motility), and the level of serum testosterone in AFB1-exposed mice. Moreover, our results showed that Se attenuated the AFB1-induced oxidative stress and the reduction of testicular testosterone synthesis enzyme protein expression such as steroidogenic acute regulatory protein (StAR), P450 side-chain cleavage (P450scc), and 17β-hydroxysteroid dehydrogenase (17β-HSD) in AFB1-exposed mice. These results demonstrated that Se conferred protection against AFB1-induced testicular toxicity and can be attributed to its antioxidant and increased testosterone level by stimulating protein expression of StAR and testosterone synthetic enzymes.

  14. An Outer Mitochondrial Translocase, Tom22, Is Crucial for Inner Mitochondrial Steroidogenic Regulation in Adrenal and Gonadal Tissues

    PubMed Central

    Rajapaksha, Maheshinie; Kaur, Jasmeet; Prasad, Manoj; Pawlak, Kevin J.; Marshall, Brendan; Perry, Elizabeth W.; Whittal, Randy M.

    2016-01-01

    After cholesterol is transported into the mitochondria of steroidogenic tissues, the first steroid, pregnenolone, is synthesized in adrenal and gonadal tissues to initiate steroid synthesis by catalyzing the conversion of pregnenolone to progesterone, which is mediated by the inner mitochondrial enzyme 3β-hydroxysteroid dehydrogenase 2 (3βHSD2). We report that the mitochondrial translocase Tom22 is essential for metabolic conversion, as its knockdown by small interfering RNA (siRNA) completely ablated progesterone conversion in both steroidogenic mouse Leydig MA-10 and human adrenal NCI cells. Tom22 forms a 500-kDa complex with mitochondrial proteins associated with 3βHSD2. Although the absence of Tom22 did not inhibit mitochondrial import of cytochrome P450scc (cytochrome P450 side chain cleavage enzyme) and aldosterone synthase, it did inhibit 3βHSD2 expression. Electron microscopy showed that Tom22 is localized at the outer mitochondrial membrane (OMM), while 3βHSD2 is localized at the inner mitochondrial space (IMS), where it interacts through a specific region with Tom22 with its C-terminal amino acids and a small amino acid segment of Tom22 exposed to the IMS. Therefore, Tom22 is a critical regulator of steroidogenesis, and thus, it is essential for mammalian survival. PMID:26787839

  15. Citrus limon extract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice.

    PubMed

    Singh, Nidhi; Singh, Shio Kumar

    2016-01-01

    The effect of oral administration of 50% ethanolic leaf extract of Citrus limon (500 and 1,000 mg/kg body weight/day) for 35 days on fertility and various male reproductive endpoints was evaluated in Parkes strain of mice. Testicular indices such as histology, 3β- and 17β-HSD enzymes activity, immunoblot expression of StAR and P450scc, and germ cell apoptosis by TUNEL and CASP- 3 expression were assessed. Motility, viability, and number of spermatozoa in the cauda epididymidis, level of serum testosterone, fertility indices, and toxicological parameters were also evaluated. Histologically, testes in extract-treated mice showed nonuniform degenerative changes in the seminiferous tubules. Treatment had adverse effects on steroidogenic markers in the testis and induced germ cell apoptosis. Significant reductions were noted in epididymal sperm parameters and serum level of testosterone in Citrus-treated mice compared to controls. Fertility of the extract-treated males was also suppressed, but libido remained unaffected. By 56 days of treatment withdrawal, alterations induced in the above parameters returned to control levels suggesting that Citrus treatment causes reversible suppression of spermatogenesis and fertility in Parkes mice. Suppression of spermatogenesis may result from germ cell apoptosis because of decreased production of testosterone. The present work indicated that Citrus leaves can affect male reproduction.

  16. Steroid exposure during larval development of Xenopus laevis affects mRNA expression of the reproductive pituitary-gonadal axis in a sex- and stage-dependent manner.

    PubMed

    Urbatzka, Ralph; Lorenz, Claudia; Wiedemann, Caterina; Lutz, Ilka; Kloas, Werner

    2014-03-01

    Steroids are known to influence the reproductive pituitary-gonadal axis in adult amphibians. Here, we studied the effects of hormones on pituitary and gonadal mRNA expression during the development of Xenopus laevis. Tadpoles at NF 58 (prometamorphosis) and at NF 66 (freshly metamorphosed) were exposed for three days to 17β-estradiol (E2), tamoxifen (TAM), testosterone (T), dihydrotestosterone (DHT) at 10(-7)M, and flutamide (FLU) at 10(-6)M. In both genders at NF 58 and 66, T and DHT decreased luteinizing hormone beta (lhβ), but increased follicle stimulating hormone beta (fshβ), while FLU induced lhβ specifically in males. In the testis steroidogenic genes (p450 side chain cleavage enzyme, p450scc; steroid acute regulatory protein, star) at NF 58 showed a similar pattern as for lhβ, while the response at NF 66 was only partially present. In females, TAM induced lhβ at NF 58, while E2 decreased lhβ and increased fshβ at NF 66. In the ovaries, no alterations were observed for the steroidogenic genes. Summarizing, gonadotropic and steroidogenic mRNA expression may indicate control of androgen level during testis differentiation in male tadpoles at NF 58. In females the non-responsiveness of steroidogenic genes could be a sign of gonadal quiescence during pre-pubertal stages.

  17. site plan, floor plan, southeast and east elevations, detail showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    site plan, floor plan, southeast and east elevations, detail showing original front entrance, interior detail showing fireplace in elevation - Neiman House, 1930 Providence Road, Charlotte, Mecklenburg County, NC

  18. 42 CFR 456.655 - Validation of showings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Validation of showings. 456.655 Section 456.655... Showing of an Effective Institutional Utilization Control Program § 456.655 Validation of showings. (a) The Administrator will periodically validate showings submitted under § 456.654. Validation...

  19. 42 CFR 456.655 - Validation of showings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 4 2011-10-01 2011-10-01 false Validation of showings. 456.655 Section 456.655... Showing of an Effective Institutional Utilization Control Program § 456.655 Validation of showings. (a) The Administrator will periodically validate showings submitted under § 456.654. Validation...

  20. TRMM Satellite Shows Bertha's Heavy Rain Pushed From Wind Shear

    NASA Video Gallery

    TRMM Satellite Shows Bertha's Heavy Rain Pushed From Wind Shear This 3-D flyby of Tropical Storm Bertha on Aug. 1 was created from TRMM satellite data. It shows (from the south) intense thunderstor...

  1. 78. VIEW OF UNCOMPLETED RESERVOIR, SHOWING FOREBAY AND FLUME; ALSO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    78. VIEW OF UNCOMPLETED RESERVOIR, SHOWING FOREBAY AND FLUME; ALSO SHOWING POOL ARRANGEMENT FOR TEMPORARILY UTILIZING WATER WITHOUT FILLING THE RESERVOIR, Print No. 232, April 1904 - Electron Hydroelectric Project, Along Puyallup River, Electron, Pierce County, WA

  2. 1. AERIAL VIEW, LOOKING NORTH, SHOWING COVERED BARGE (VESSEL 37) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. AERIAL VIEW, LOOKING NORTH, SHOWING COVERED BARGE (VESSEL 37) IN CENTER OF PICTURE WITH FOUR HATCHES SHOWING IN SUPERSTRUCTURE Charles Wisniewski, photographer, January 1985 - Shooters Island, Ships Graveyard, Vessel No. 37, Newark Bay, Staten Island (subdivision), Richmond County, NY

  3. 22. DETAIL EXTERIOR VIEW LOOKING NORTHWEST, SHOWING FISH LADDER AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. DETAIL EXTERIOR VIEW LOOKING NORTHWEST, SHOWING FISH LADDER AT NORTH END OF DAM/SPILLWAY; VIEW SHOWS SECTION OF FISH LADDER NEAR WHERE IT ENTERS THE COLUMBIA RIVER. - Bonneville Project, Bonneville Dam, Columbia River, Bonneville, Multnomah County, OR

  4. 16. SECOND FLOOR, EAST ROOM, SHOWING DOORWAYS TO BOXED WINDER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. SECOND FLOOR, EAST ROOM, SHOWING DOORWAYS TO BOXED WINDER STAIRWAYS-- UP AND DOWN-- AND WALL CABINET WITH DOORS OPEN TO SHOW STAIRWAY - High Farm, House, Creek Road, 1 mile West of Easton Road, Pipersville, Bucks County, PA

  5. 94. View looking south showing foundation equipment at work on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    94. View looking south showing foundation equipment at work on two of the piers. The view also shows the two completed cylinders in the midstream cluster of four. - Carquinez Bridge, Spanning Carquinez Strait at Interstate 80, Vallejo, Solano County, CA

  6. 21 CFR 1314.150 - Order To show cause.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Order To show cause. 1314.150 Section 1314.150 Food and Drugs DRUG ENFORCEMENT ADMINISTRATION, DEPARTMENT OF JUSTICE RETAIL SALE OF SCHEDULED LISTED CHEMICAL PRODUCTS Order to Show Cause § 1314.150 Order To show cause. (a) If, upon information gathered...

  7. 20. MEMBER 'A' SHOWS TENON AS USED IN POST 'A' ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. MEMBER 'A' SHOWS TENON AS USED IN POST 'A' (TN-159A-19), MEMBER 'B' IS BEAM 'B' IN TN-159A-19 AND SHOWS METHOD OF JOINING THESE MEMBERS. MEMBER 'C' SHOWS MORTISE IN BEAM 'B'. - Caleb Crosby Threshing Barn, Noeton (moved to Norris Dam State Park, Lake City), Morristown, Hamblen County, TN

  8. 56. View looking east. Detail showing an end of three ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. View looking east. Detail showing an end of three crib side walls, being flown by the derrick. The upper part of the fourth member, marked with an identifying tag, shows evidence of burning. Notice how well the lower members fit together. This view shows that individual timber members were worked, in part, after assembly. - Wabash & Erie Canal, Lock No. 2, 8 miles east of Fort Wayne, adjacent to U.S. Route 24, New Haven, Allen County, IN

  9. 52. View from ground level showing lower radar scanner switch ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    52. View from ground level showing lower radar scanner switch with open port door in radar scanner building 105 showing emanating waveguides from lower switch in vertical run; photograph also shows catwalk to upper scanner switch in upper left side of photograph and structural supports. - Clear Air Force Station, Ballistic Missile Early Warning System Site II, One mile west of mile marker 293.5 on Parks Highway, 5 miles southwest of Anderson, Anderson, Denali Borough, AK

  10. 26. VIEW, LOOKING NORTHWEST INSIDE TRANSFORMER ROOM, SHOWING OIL FILLED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. VIEW, LOOKING NORTHWEST INSIDE TRANSFORMER ROOM, SHOWING OIL- FILLED TRANSFORMER POTS - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA

  11. 4. View facing southwest showing the Silvertop Diner, Providence Fruit ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. View facing southwest showing the Silvertop Diner, Providence Fruit & Produce Building, and Merchants' Cold Storage Warehouse. - Provisions Warehouse Historic District, Kinsley & Harris Avenues, Providence, Providence County, RI

  12. 29. Detail view north showing amperage and voltage meters, operator's ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    29. Detail view north showing amperage and voltage meters, operator's room, west operator's house. - Yellow Mill Bridge, Spanning Yellow Mill Channel at Stratford Avenue, Bridgeport, Fairfield County, CT

  13. 11. Exterior detail view of northeast corner, showing stucco finish ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Exterior detail view of northeast corner, showing stucco finish and woodwork details - American Railway Express Company Freight Building, 1060 Northeast Division Street, Bend, Deschutes County, OR

  14. EAST AND NORTH SIDES OF BLOWER HOUSE SHOWING POWER WHEELS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    EAST AND NORTH SIDES OF BLOWER HOUSE SHOWING POWER WHEELS AND RACEWAY, LOOKING SOUTH. - Tannehill Furnace, 12632 Confederate Parkway, Tannehill Historical State Park, Bucksville, Tuscaloosa County, AL

  15. 17. VIEW LOOKING NORTHEAST, SHOWING SORTING AND SHIPPING SHED WITH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. VIEW LOOKING NORTHEAST, SHOWING SORTING AND SHIPPING SHED WITH SAWMILL BEHIND - Ichabod T. Williams & Sons Sawmill & Veneer Plant, Roosevelt Avenue at Carteret Avenue, Carteret, Middlesex County, NJ

  16. 25. VIEW, LOOKING SOUTHWEST INSIDE TRANSFORMER ROOM, SHOWING TRANSFORMERS AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. VIEW, LOOKING SOUTHWEST INSIDE TRANSFORMER ROOM, SHOWING TRANSFORMERS AND KNIFE SWITCHES - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA

  17. Contextual view showing northeastern eucalyptus windbreak and portion of citrus ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Contextual view showing northeastern eucalyptus windbreak and portion of citrus orchard. Camera facing 118" east-southeast. - Goerlitz House, 9893 Highland Avenue, Rancho Cucamonga, San Bernardino County, CA

  18. 14. View showing detail of truss (unidentified). Drawing courtesy Engineering ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View showing detail of truss (unidentified). Drawing courtesy Engineering Department, City of Cleveland. - Superior Avenue Viaduct, Cleveland East & West side, Cuyahoga Valley Vicinity, Cleveland, Cuyahoga County, OH

  19. 4. PERSPECTIVE VIEW OF MAIN AND EAST ELEVATIONS, SHOWING VIEW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. PERSPECTIVE VIEW OF MAIN AND EAST ELEVATIONS, SHOWING VIEW TOWARD CARPENTER'S HALL - Carpenters' Company, Front Store, 322 Chestnut Street & Carpenters' Court, Philadelphia, Philadelphia County, PA

  20. 54. VIEW SHOWING THE PLACEMENT OF SPIDER WEB BRACING, SHOOFLY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    54. VIEW SHOWING THE PLACEMENT OF SPIDER WEB BRACING, SHOOFLY BRIDGE, January 1935 - Sacramento River Bridge, Spanning Sacramento River at California State Highway 275, Sacramento, Sacramento County, CA

  1. 246. View showing the curvilinear alignment of the parkway on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    246. View showing the curvilinear alignment of the parkway on a ridge. Looking northeast. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  2. 5. VIEW, LOOKING NORTHEAST, SHOWING BRIDGE COUNTERWEIGHT New York, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. VIEW, LOOKING NORTHEAST, SHOWING BRIDGE COUNTERWEIGHT - New York, New Haven & Hartford Railroad, Niantic Bridge, Spanning Niantic River between East Lyme & Waterford, Old Lyme, New London County, CT

  3. 2. GENERAL VIEW FROM SOUTH SHOWING SOUTHWEST AND SOUTHEAST SIDES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. GENERAL VIEW FROM SOUTH SHOWING SOUTHWEST AND SOUTHEAST SIDES AND CLERESTORY ARRANGEMENT - Sulphur Springs Methodist Campground, Sulphur Springs Road (Sulphur Springs), Sulphur Springs, Washington County, TN

  4. New rain shed (Building No. 241) interior showing posts, braces, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    New rain shed (Building No. 241) interior showing posts, braces, and roof structure. - Hawaii Volcanoes National Park Water Collection System, Hawaii Volcanoes National Park, Volcano, Hawaii County, HI

  5. 13. VIEW INTO BLOCK AREA SHOWING KEY MECHANISM, NOTE FLOOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW INTO BLOCK AREA SHOWING KEY MECHANISM, NOTE FLOOR SEPARATION AT THRESHOLD AND KEY-WINDING MECHANISM - Montgomery County Jail, Washington & Spring Streets, Crawfordsville, Montgomery County, IN

  6. 1. Aerial view, looking northeast up Newark Bay, showing entire ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Aerial view, looking northeast up Newark Bay, showing entire island Charles Wisniewski, photographer, January 1985 - Shooters Island, Ships Graveyard, Newark Bay, Staten Island (subdivision), Richmond County, NY

  7. PERSPECTIVE VIEW SHOWING SOUTH SIDE OF BUILDING, LOOKING WESTNORTHWEST DOWN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PERSPECTIVE VIEW SHOWING SOUTH SIDE OF BUILDING, LOOKING WEST-NORTHWEST DOWN HARRISON AVENUE - Pearce Manufacturing Company, Factory A, Harrison Avenue West at Wilkens, Latrobe, Westmoreland County, PA

  8. INTERIOR VIEW OF ENTRANCE TO LABORATORY, SHOWING HANDHAMMERED ALUMINUM DOORS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF ENTRANCE TO LABORATORY, SHOWING HAND-HAMMERED ALUMINUM DOORS AND MARBLE. NOTE ALUMINUM LIGHT FIXTURE - Alcoa Research Laboratory, Freeport Road, New Kensington, Westmoreland County, PA

  9. Interior view, detail of the staircase to show the burnished ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, detail of the staircase to show the burnished aluminum and brass balustrade - Departmental Auditorium, Constitution Avenue between Twelfth and Fourteenth Streets, Washington, District of Columbia, DC

  10. 7. Photocopy of photograph (from Broome County Historical Society) showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photocopy of photograph (from Broome County Historical Society) showing SWIMMERS, PHOTOGRAPH TAKEN FACING NORTHEAST - Charles F. Johnson Pool, Charles F. Johnson Park, Johnson City, Broome County, NY

  11. 5. GENERAL VIEW, SECOND FLOOR, SHOWING TYPICAL WINDOW TRIM AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. GENERAL VIEW, SECOND FLOOR, SHOWING TYPICAL WINDOW TRIM AND THE PRESSED METAL CEILING, VIEW FROM SOUTHWEST. - 443 Seventh Street, Northwest (Commercial Building), Washington, District of Columbia, DC

  12. 23. VIEW SHOWING SALT RIVER PROJECT CREWS SLIPFORMING LATERAL DURING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. VIEW SHOWING SALT RIVER PROJECT CREWS SLIPFORMING LATERAL DURING REHABILITATION AND BETTERMENT PROGRAM Photographer: unknown. April 1968 - Arizona Canal, North of Salt River, Phoenix, Maricopa County, AZ

  13. 1. Southeast elevation of Oil House showing loading platform. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Southeast elevation of Oil House showing loading platform. - Delaware, Lackawanna & Western Railroad, Scranton Yards, Oil House, 650 feet Southeast of Cliff & Mechanic Streets, Scranton, Lackawanna County, PA

  14. 24. CONSTRUCTION PROGRESS VIEW TO NORTHWEST, SHOWING BLOWER BUILDING. INEEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. CONSTRUCTION PROGRESS VIEW TO NORTHWEST, SHOWING BLOWER BUILDING. INEEL PHOTO NUMBER NRTS-60-4407. - Idaho National Engineering Laboratory, Old Waste Calcining Facility, Scoville, Butte County, ID

  15. Detail view of upper southwest corner, showing representative view of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of upper southwest corner, showing representative view of cornice and window ornamentation - Hungarian Sick Benefit Societies Building, 1406-1418 State Street, Bridgeport, Fairfield County, CT

  16. 13. FOURTH FLOOR ROASTING ROOM, SHOWING CLERESTORY. VIEW TO SOUTH. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. FOURTH FLOOR ROASTING ROOM, SHOWING CLERESTORY. VIEW TO SOUTH. - Commercial & Industrial Buildings, McFadden Coffee & Spice Company, Factory & Warehouse, 145 First Street, Dubuque, Dubuque County, IA

  17. 12. TRANSMISSION GEARING SHOWING RELATION TO SEGMENT GEAR ON WATERWHEEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. TRANSMISSION GEARING SHOWING RELATION TO SEGMENT GEAR ON WATERWHEEL william E. Barrett, photographer, 1973 (copy negative) - Thomas Shepherd's Grist Mill, High Street Vicinity, Shepherdstown, Jefferson County, WV

  18. 2. VIEW SOUTH SHOWING NORTHEAST ELEVATION; BRICK CORBELLING, BUTTRESSES AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW SOUTH SHOWING NORTHEAST ELEVATION; BRICK CORBELLING, BUTTRESSES AND ART DECO STAINED GLASS - Poletown Historic District, St. Michael's Greek Catholic Church, 2390 East Grand Boulevard, Detroit, MI

  19. 6. SOUTHEAST ABUTMENT AT CALVERT STREET, SHOWING LEON HERMANT ALLEGORICAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. SOUTHEAST ABUTMENT AT CALVERT STREET, SHOWING LEON HERMANT ALLEGORICAL RELIEF OF TRANSPORTATION BY AUTOMOBILE - Calvert Street Bridge, Spanning Rock Creek & Potomac Parkway, Washington, District of Columbia, DC

  20. 25. DETAIL SHOWING BRAKING MECHANISM FOR TRAIN, NOTE HOT RAIL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. DETAIL SHOWING BRAKING MECHANISM FOR TRAIN, NOTE HOT RAIL ON LEFT - Jefferson National Expansion Memorial Arch, Mississippi River between Washington & Poplar Streets, Saint Louis, Independent City, MO

  1. 3. Photocopy of 1932 photograph showing another general view of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of 1932 photograph showing another general view of the mansion, looking northwest. Original photograph at the Philadelphia Museum of Art. - Strawberry Mansion, Philadelphia, Philadelphia County, PA

  2. 13. Detail showing canopy at southeast corner; note single column ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Detail showing canopy at southeast corner; note single column supporting structure - Fort Hood, World War II Temporary Buildings, Cold Storage Building, Seventeenth Street, Killeen, Bell County, TX

  3. Time dependent patient no-show predictive modelling development.

    PubMed

    Huang, Yu-Li; Hanauer, David A

    2016-05-09

    Purpose - The purpose of this paper is to develop evident-based predictive no-show models considering patients' each past appointment status, a time-dependent component, as an independent predictor to improve predictability. Design/methodology/approach - A ten-year retrospective data set was extracted from a pediatric clinic. It consisted of 7,291 distinct patients who had at least two visits along with their appointment characteristics, patient demographics, and insurance information. Logistic regression was adopted to develop no-show models using two-thirds of the data for training and the remaining data for validation. The no-show threshold was then determined based on minimizing the misclassification of show/no-show assignments. There were a total of 26 predictive model developed based on the number of available past appointments. Simulation was employed to test the effective of each model on costs of patient wait time, physician idle time, and overtime. Findings - The results demonstrated the misclassification rate and the area under the curve of the receiver operating characteristic gradually improved as more appointment history was included until around the 20th predictive model. The overbooking method with no-show predictive models suggested incorporating up to the 16th model and outperformed other overbooking methods by as much as 9.4 per cent in the cost per patient while allowing two additional patients in a clinic day. Research limitations/implications - The challenge now is to actually implement the no-show predictive model systematically to further demonstrate its robustness and simplicity in various scheduling systems. Originality/value - This paper provides examples of how to build the no-show predictive models with time-dependent components to improve the overbooking policy. Accurately identifying scheduled patients' show/no-show status allows clinics to proactively schedule patients to reduce the negative impact of patient no-shows.

  4. 13. VIEW SHOWING MOST OF THE PERIMETER FROM SPILLWAY BOX ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW SHOWING MOST OF THE PERIMETER FROM SPILLWAY BOX TO END OF EAST DAM. FOREGROUND VIEW SHOWS TRIPLE WALL CONSTRUCTION OF TONGUE AND GROOVE PLANKING USED IN CRIBBING - Three Bears Lake & Dams, North of Marias Pass, East Glacier Park, Glacier County, MT

  5. 2. DETAIL VIEW SHOWING WOODEN CRIBBING WITH LOWERED LAKE LEVEL, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. DETAIL VIEW SHOWING WOODEN CRIBBING WITH LOWERED LAKE LEVEL, EAST DAM, LOOKING NORTHEAST (View is middle of the perimeter showing in MT-88-A-1 above.) - Three Bears Lake & Dams, East Dam, North of Marias Pass, East Glacier Park, Glacier County, MT

  6. 47 CFR 101.411 - Supplementary showing required.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Supplementary showing required. 101.411 Section 101.411 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES FIXED MICROWAVE SERVICES Developmental Authorizations § 101.411 Supplementary showing required....

  7. Cross Section; Half Longitudinal Section Showing Middle Wall Reinforced with ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Cross Section; Half Longitudinal Section Showing Middle Wall Reinforced with Arch; Part Long Section Showing Inside of External Side Wall; East Entrance; Part Side South External; Part Reflected Plan of Soffite of Floor; Part Reflected Plan of Soffite of Roof - Blenheim Covered Bridge, Spanning Schoharie River, North Blenheim, Schoharie County, NY

  8. Showing and Telling: The Difference That Makes a Difference.

    ERIC Educational Resources Information Center

    Lewis, David

    2001-01-01

    Attempts to clarify an essential difference between the ways in which pictures and words convey meaning. Examines one attempt to differentiate and characterize various types of picture books and concludes by showing how Anthony Browne exploits the distinction between showing and telling to create the atmosphere of uncertainty and mystery in his…

  9. 47 CFR 73.33 - Antenna systems; showing required.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 4 2010-10-01 2010-10-01 false Antenna systems; showing required. 73.33... RADIO BROADCAST SERVICES AM Broadcast Stations § 73.33 Antenna systems; showing required. (a) An application for authority to install a broadcast antenna shall specify a definite site and include...

  10. 47 CFR 73.33 - Antenna systems; showing required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 4 2011-10-01 2011-10-01 false Antenna systems; showing required. 73.33... RADIO BROADCAST SERVICES AM Broadcast Stations § 73.33 Antenna systems; showing required. (a) An application for authority to install a broadcast antenna shall specify a definite site and include...

  11. 47 CFR 73.33 - Antenna systems; showing required.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 4 2014-10-01 2014-10-01 false Antenna systems; showing required. 73.33... RADIO BROADCAST SERVICES AM Broadcast Stations § 73.33 Antenna systems; showing required. (a) An application for authority to install a broadcast antenna shall specify a definite site and include...

  12. 47 CFR 73.33 - Antenna systems; showing required.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 4 2013-10-01 2013-10-01 false Antenna systems; showing required. 73.33... RADIO BROADCAST SERVICES AM Broadcast Stations § 73.33 Antenna systems; showing required. (a) An application for authority to install a broadcast antenna shall specify a definite site and include...

  13. 47 CFR 73.33 - Antenna systems; showing required.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 4 2012-10-01 2012-10-01 false Antenna systems; showing required. 73.33... RADIO BROADCAST SERVICES AM Broadcast Stations § 73.33 Antenna systems; showing required. (a) An application for authority to install a broadcast antenna shall specify a definite site and include...

  14. 31. Photographic copy of drawing showing profile of bridge after ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. Photographic copy of drawing showing profile of bridge after the 1888-1889 and 1899-1900 reconstructions; also shows profile of bridge before 1888 (Martin Sigvart Grytbak, Wabasha St. Bridge, Formerly St. Paul Bridge, 1919); profile of Wabasha street bridge - Wabasha Street Bridge, Spanning Mississippi River at Wabasha Street, Saint Paul, Ramsey County, MN

  15. 15. BALD MOUNTAIN MILL, INTERIOR SHOWING PRECIPITATION AREA FROM NORTH, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. BALD MOUNTAIN MILL, INTERIOR SHOWING PRECIPITATION AREA FROM NORTH, c. 1934. SHOWS PRECIPITATION TANK No. 1 (NOTE LOCKS), ZINC FEEDER WITH MIXING CONE, VACUUM RECEIVER AND PIPING. CREDIT WR. - Bald Mountain Gold Mill, Nevada Gulch at head of False Bottom Creek, Lead, Lawrence County, SD

  16. 77 FR 1513 - Air Show and Air Races; Public Hearing

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-10

    ... From the Federal Register Online via the Government Publishing Office NATIONAL TRANSPORTATION SAFETY BOARD Air Show and Air Races; Public Hearing TIME AND DATE: 9 a.m., Tuesday, January 10, 2012... hearing is to examine current regulations and oversight practices for air shows and air races,...

  17. TV shows on Light Pollution Education for the Public

    NASA Astrophysics Data System (ADS)

    Grigore, Valentin

    2015-03-01

    TV shows have the biggest impact for the public, so we can use them to inform and educate the public about light pollution and the importance of the dark sky for humanity and for the contemporary society. Some examples used in the TV show Us and the Sky at Columna TV, Romania, are presented.

  18. Survey Shows Blacks Not Concerned Enough about Kidney Disease

    ERIC Educational Resources Information Center

    Black Issues in Higher Education, 2004

    2004-01-01

    Health officials may have an uphill battle in educating Blacks about a disease that's being called a "silent killer," a recent survey shows. Kidney disease is an illness that's become more prevalent, especially in the nation's Black population, but a survey conducted in Jackson, Atlanta, Baltimore and Cleveland shows only 15 percent of those…

  19. Computer Slide Shows: A Trap for Bad Teaching

    ERIC Educational Resources Information Center

    Klemm, W. R.

    2007-01-01

    Slide shows presented with software such as PowerPoint or WordPerfect Presentations can trap instructors into bad teaching practices. Research on memory suggests that slide-show instruction can actually be less effective than traditional lecturing when the teacher uses a blackboard or overhead projector. The author proposes a model of classroom…

  20. The Easy Way to Create Computer Slide Shows.

    ERIC Educational Resources Information Center

    Anderson, Mary Alice

    1995-01-01

    Discusses techniques for creating computer slide shows. Topics include memory; format; color use; HyperCard and CD-ROM; font styles and sizes; graphs and graphics; the slide show option; special effects; and tips for effective presentation. (Author/AEF)

  1. 43 CFR 3430.2-1 - Initial showing.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-1 Initial showing. All preference right coal lease applications shall have contained or shall have... analysis, sulfur content and BTU content of the coal, and all supporting geological and geophysical data... to be mined by surface mining methods, isopachous maps of the overburden. These maps shall show...

  2. The Presentation of Science in Everyday Life: The Science Show

    ERIC Educational Resources Information Center

    Watermeyer, Richard

    2013-01-01

    This paper constitutes a case-study of the "science show" model of public engagement employed by a company of science communicators focused on the popularization of science, technology, engineering and mathematics (STEM) subject disciplines with learner constituencies. It examines the potential of the science show to foster the interest…

  3. 47 CFR 73.24 - Broadcast facilities; showing required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 4 2011-10-01 2011-10-01 false Broadcast facilities; showing required. 73.24... RADIO BROADCAST SERVICES AM Broadcast Stations § 73.24 Broadcast facilities; showing required. An authorization for a new AM broadcast station or increase in facilities of an existing station will be...

  4. 47 CFR 101.411 - Supplementary showing required.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Supplementary showing required. 101.411 Section 101.411 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES FIXED MICROWAVE SERVICES Developmental Authorizations § 101.411 Supplementary showing required....

  5. 47 CFR 101.411 - Supplementary showing required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Supplementary showing required. 101.411 Section 101.411 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES FIXED MICROWAVE SERVICES Developmental Authorizations § 101.411 Supplementary showing required....

  6. "The Daily Show with Jon Stewart": Part 1

    ERIC Educational Resources Information Center

    Trier, James

    2008-01-01

    Comedy Central's popular program "The Daily Show With Jon Stewart" is the best critical media literacy program on television, and it can be used in valuable ways in the classroom as part of a media literacy pedagogy. This Media Literacy column provides an overview of the show and its accompanying website and considers ways it might be used in the…

  7. The Daily Show with Jon Stewart: Part 2

    ERIC Educational Resources Information Center

    Trier, James

    2008-01-01

    "The Daily Show With Jon Stewart" is one of the best critical literacy programs on television, and in this Media Literacy column the author suggests ways that teachers can use video clips from the show in their classrooms. (For Part 1, see EJ784683.)

  8. View of Lake Sabrina Dam showing wooden planks along the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of Lake Sabrina Dam showing wooden planks along the upstream face and concrete base added in 1916/1917 and showing the iron grating covering upstream side of outlet structure is visible at lower photo center, view northeast - Bishop Creek Hydroelectric System, Plant 2, Lake Sabrina Dam, Bishop Creek, Bishop, Inyo County, CA

  9. 28. VIEW SOUTHWEST, EAST ABUTMENT SHOWING BASCULE LEAF IN RAISED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. VIEW SOUTHWEST, EAST ABUTMENT SHOWING BASCULE LEAF IN RAISED POSITION WITH THE EXTREME UPPER PORTION OF THE BASCULE RACK GEAR SHOWING IN THE LOWER RIGHT PORTION OF THE PHOTOGRAPH - Tomlinson Bridge, Spanning Quinnipiac River at Forbes Street (U.S. Route 1), New Haven, New Haven County, CT

  10. 108. View showing storage yard where material is received and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    108. View showing storage yard where material is received and sorted: also shows derrick framed to raise material from tracks and land on deck of approach. Material is then moved by narrow gage locomotive out to erection traveler. - Carquinez Bridge, Spanning Carquinez Strait at Interstate 80, Vallejo, Solano County, CA

  11. 27. GENERAL INTERIOR VIEW SHOWING SOUTH CORNER OF SHED WITH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. GENERAL INTERIOR VIEW SHOWING SOUTH CORNER OF SHED WITH ONE-STORY OFFICES, SHOWING TYPICAL COLUMN BASE WITH TIMBER BOLTED TO STEEL 'L' SHOE - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

  12. 15. Photocopy of historic view No. 31001 showing expansion of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Photocopy of historic view No. 31001 showing expansion of Building 461 (9/4/56) showing Building 463 tanks to the right of the photo. Original is on file Mare Island Naval Shipyard-Photography Lab. Photographer unknown. - Mare Island Naval Shipyard, Acid Mixing Facility, California Avenue & E Street, Vallejo, Solano County, CA

  13. Game Show Mathematics: Specializing, Conjecturing, Generalizing, and Convincing

    ERIC Educational Resources Information Center

    Lane, Catherine Pullin; Harkness, Shelly Sheats

    2012-01-01

    This article describes the authors' use of three game shows--"Survivor," "The Biggest Loser," and "Deal or No Deal?"--to determine to what degree students engaged in mathematical thinking: specializing, conjecturing, generalizing, and convincing (Burton, 1984). Student responses to the task of creating winning strategies to these shows were…

  14. ShowFlow: A practical interface for groundwater modeling

    SciTech Connect

    Tauxe, J.D.

    1990-12-01

    ShowFlow was created to provide a user-friendly, intuitive environment for researchers and students who use computer modeling software. What traditionally has been a workplace available only to those familiar with command-line based computer systems is now within reach of almost anyone interested in the subject of modeling. In the case of this edition of ShowFlow, the user can easily experiment with simulations using the steady state gaussian plume groundwater pollutant transport model SSGPLUME, though ShowFlow can be rewritten to provide a similar interface for any computer model. Included in this thesis is all the source code for both the ShowFlow application for Microsoft{reg sign} Windows{trademark} and the SSGPLUME model, a User's Guide, and a Developer's Guide for converting ShowFlow to run other model programs. 18 refs., 13 figs.

  15. Dolphin shows and interaction programs: benefits for conservation education?

    PubMed

    Miller, L J; Zeigler-Hill, V; Mellen, J; Koeppel, J; Greer, T; Kuczaj, S

    2013-01-01

    Dolphin shows and dolphin interaction programs are two types of education programs within zoological institutions used to educate visitors about dolphins and the marine environment. The current study examined the short- and long-term effects of these programs on visitors' conservation-related knowledge, attitude, and behavior. Participants of both dolphin shows and interaction programs demonstrated a significant short-term increase in knowledge, attitudes, and behavioral intentions. Three months following the experience, participants of both dolphin shows and interaction programs retained the knowledge learned during their experience and reported engaging in more conservation-related behaviors. Additionally, the number of dolphin shows attended in the past was a significant predictor of recent conservation-related behavior suggesting that repetition of these types of experiences may be important in inspiring people to conservation action. These results suggest that both dolphin shows and dolphin interaction programs can be an important part of a conservation education program for visitors of zoological facilities.

  16. Snacking on Television: A Content Analysis of Adolescents’ Favorite Shows

    PubMed Central

    Larson, Nicole I.; Gollust, Sarah E.; Neumark-Sztainer, Dianne

    2016-01-01

    Introduction Snacking is a complex behavior that may be influenced by entertainment media. Research suggests that snacking and unhealthy foods are commonly shown in programming that targets young audiences, but shows selected for study have been limited. We conducted a content analysis on shows that were named as favorites by adolescents to characterize portrayals of snacking on popular television. Methods A diverse sample of 2,130 adolescents (mean age, 14.3 y) listed 3 favorite television shows in a 2010 school-based survey. Three episodes each of the 25 most popular shows were coded for food-related content, including healthfulness, portion size, screen time use, setting, and social context. We also analyzed the characteristics of characters involved in eating incidents, the show type, and the show rating. We used χ2 tests, binomial tests, and multilevel regression models to compare incidence of snacks versus meals, the characteristics of those involved, and snacking across show characteristics. Results Almost half of food incidents on television shows were snacks. Snacks were significantly more likely than meals to be “mostly unhealthy” (69.3% vs 22.6%, P < .001) and were more likely to include screen time use (25.0% of snacking incidents vs 4.0% of meals, P < .001). Young characters and those coded as being of low socioeconomic status or overweight were overrepresented in snacking incidents. Sitcoms and shows rated for a youth audience were significantly more likely to portray snacking than were shows for adult audiences. Conclusion Media awareness and literacy programs should include foods and snacking behaviors among the issues they address. More healthful portrayals of food and dietary intake in entertainment shows’ content would create a healthier media environment for youth. PMID:27197079

  17. 1. DISTANT VIEW OF FRONT FROM SOUTHWEST SHOWING AREA OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. DISTANT VIEW OF FRONT FROM SOUTHWEST SHOWING AREA OF COUNCIL CHAMBERS, SHOP WITH PORTION OF WATER TREATMENT PLANT AND TRACK FILL AREA IN RIGHT HAND CORNER - Hardin City Water Works, 101 East Fourth Street, Hardin, Big Horn County, MT

  18. 2. AERIAL VIEW LOOKING WEST SOUTHWEST SHOWING DOLPHIN MANUFACTURING CO., ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. AERIAL VIEW LOOKING WEST SOUTHWEST SHOWING DOLPHIN MANUFACTURING CO., BARBOUR FLAX SPINNING CO. -- SPRUCE ST. MILL, ROGERS LOCOMOTIVE AND MACHINE WORKS -- MILLWRIGHT SHOP AND FITTING SHOP. - Great Falls S. U. M. Historic District, Oliver Street, Paterson, Passaic County, NJ

  19. 42. View forward on afterdeck, showing steering gear box in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    42. View forward on afterdeck, showing steering gear box in foreground, aft elevation of cabin beyond; weather canopy overhead is not an original or permanent feature - Schooner WAWONA, 1018 Valley Street, Seattle, King County, WA

  20. PERSPECTIVE FROM SOUTH SHOWING WEATHERED BOARD AND BATTEN SIDING. NOTE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PERSPECTIVE FROM SOUTH SHOWING WEATHERED BOARD AND BATTEN SIDING. NOTE BRIDGE SITS ON ONE CONCRETEFACED ABUTMENT AND ONE STONE PIER. - Beaverkill Bridge, Spanning Beaver Kill, TR 30 (Craigie Claire Road), Roscoe, Sullivan County, NY

  1. 17. Building 202, observation room for test cell, showing panel, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Building 202, observation room for test cell, showing panel, abort button, phones, and observation window. View looking northwest. - Rocket Engine Testing Facility, GRC Building No. 202, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH

  2. Detail of smokestack base on east side, showing door manufactured ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of smokestack base on east side, showing door manufactured by Heine Chimney Co., Chicago. - Fitzsimons General Hospital, Power House, Northwest Corner of East Harlow Avenue & North Page Street, Aurora, Adams County, CO

  3. North side, showing ramp at western section but photograph taken ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North side, showing ramp at western section but photograph taken to east of CO-172-BR-8 and looking southwesterly. - Fitzsimons General Hospital, Infirmary, Northwest Corner of East Bushnell Avenue & South Page Street, Aurora, Adams County, CO

  4. East side, middle section, looking southwest, shows slightly more northerly ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    East side, middle section, looking southwest, shows slightly more northerly section than CO-172-AO-4. - Fitzsimons General Hospital, Quartermaster's Storehouse, Southwest Corner of East I Avenue & North Twelfth Street, Aurora, Adams County, CO

  5. 4. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO EAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO EAST. - Rocky Mountain Arsenal, Laboratory Building, 510 feet South of December Seventh Avenue; 175 feet East of D Street, Commerce City, Adams County, CO

  6. 3. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO SOUTHEAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO SOUTHEAST. - Rocky Mountain Arsenal, Laboratory Building, 510 feet South of December Seventh Avenue; 175 feet East of D Street, Commerce City, Adams County, CO

  7. 6. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO NORTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO NORTHWEST. - Rocky Mountain Arsenal, Laboratory Building, 510 feet South of December Seventh Avenue; 175 feet East of D Street, Commerce City, Adams County, CO

  8. 5. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO SOUTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO SOUTHWEST. - Rocky Mountain Arsenal, Laboratory Building, 510 feet South of December Seventh Avenue; 175 feet East of D Street, Commerce City, Adams County, CO

  9. 7. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO NORTHEAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. INTERIOR OF BUILDING 313, SHOWING LABORATORY. VIEW TO NORTHEAST. - Rocky Mountain Arsenal, Laboratory Building, 510 feet South of December Seventh Avenue; 175 feet East of D Street, Commerce City, Adams County, CO

  10. 12. OFFSHORE VIEW OF PIER, LOOKING EAST, SHOWING LIFEGUARD TOWER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. OFFSHORE VIEW OF PIER, LOOKING EAST, SHOWING LIFEGUARD TOWER AND 2ND TEE (CENTER), REFUGE BAY (RIGHT) - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  11. 11. GROUND VIEW OF PIER, LOOKING SOUTH FROM WATERLINE; SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. GROUND VIEW OF PIER, LOOKING SOUTH FROM WATERLINE; SHOWING LIFEGUARD TOWER TO END OF PIER - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  12. 14. OFFSHORE VIEW OF PIER, LOOKING SOUTHEAST, SHOWING (LEFTRIGHT) PUMPHOUSE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. OFFSHORE VIEW OF PIER, LOOKING SOUTHEAST, SHOWING (LEFT-RIGHT) PUMPHOUSE, TACKLE BOX, RESTROOMS ON 3RD TEE - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  13. 22. GROUND VIEW OF PIERS, LOOKING SOUTHEAST, SHOWING BENTS 13, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. GROUND VIEW OF PIERS, LOOKING SOUTHEAST, SHOWING BENTS 1-3, STAIRWAY (RIGHT), RESTROOMS UNDER PIER (CENTER), MAXWELL'S RESTAURANT IN BACKGROUND - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  14. Oblique view showing western abutment, looking ENE along main line. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view showing western abutment, looking ENE along main line. Septa commuter train in foreground. - Pennsylvania Railroad, Whitford Bridge, Spanning Amtrak tracks at Whitford Road, Whitford, Chester County, PA

  15. 4. Building 11 north elevation oblique, showing detail of concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Building 11 north elevation oblique, showing detail of concrete landings, window treatments. Very obscured by unremovable vegetation. View looking west. - John & James Dobson Carpet Mill (West Parcel), Building No. 11, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  16. 3. Front of Mansion, facing east, shows portico, raised section ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Front of Mansion, facing east, shows portico, raised section of second story, and section (south of extreme left chimney) added c. 1914. Winter view. - Sotterly, State Route 245 & Vista Road Vicinity, Hollywood, St. Mary's County, MD

  17. 3. HJELM FARMSTEAD. OVERVIEW SHOWING EDGE OF GRANARY, SMALL BARN, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. HJELM FARMSTEAD. OVERVIEW SHOWING EDGE OF GRANARY, SMALL BARN, WINDMILL, AND POTATO CELLAR. VIEW TO SOUTH-SOUTHEAST. - Hjelm Farmstead, U.S. Highway 20 at New Sweden, Idaho Falls, Bonneville County, ID

  18. 3. VIEW, LOOKING NORTHEAST, SHOWING A SMALL FIELDSTONE DAM (KNOWN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW, LOOKING NORTHEAST, SHOWING A SMALL FIELD-STONE DAM (KNOWN LOCALLY AS DAM NO. 2), BUILT BY THE CCC - J. Clark Salyer National Wildlife Refuge Dams, Along Lower Souris River, Kramer, Bottineau County, ND

  19. 8. VIEW OF DAM 83, SHOWING OLD SOURIS RIVER CHANNEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW OF DAM 83, SHOWING OLD SOURIS RIVER CHANNEL FROM THE DOWNSTREAM FACE OF THE DAM WITH POND A IN THE BACKGROUND, LOOKING SOUTH - Upper Souris National Wildlife Refuge, Dam 83, Souris River Basin, Foxholm, Surrey (England), ND

  20. 11. VIEW OF SPILLWAY AT DAM 83, SHOWING REFUGE HEADQUARTERS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. VIEW OF SPILLWAY AT DAM 83, SHOWING REFUGE HEADQUARTERS ON THE HORIZON (LEFT, CENTER), LOOKING EAST - Upper Souris National Wildlife Refuge, Dam 83, Souris River Basin, Foxholm, Surrey (England), ND

  1. 9. VIEW OF SPILLWAY AT DAM 83, SHOWING LOCATION OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW OF SPILLWAY AT DAM 83, SHOWING LOCATION OF FORMER CONCRETE FLASHBOARD STRUCTURE ON RIGHT, LOOKING WEST - Upper Souris National Wildlife Refuge, Dam 83, Souris River Basin, Foxholm, Surrey (England), ND

  2. 4. VIEW, LOOKING SOUTHWEST, SHOWING A LARGE FIELDSTONE DAM (KNOWN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW, LOOKING SOUTHWEST, SHOWING A LARGE FIELD-STONE DAM (KNOWN LOCALLY AS DAM NO. 1), BUILT BY THE CCC - J. Clark Salyer National Wildlife Refuge Dams, Along Lower Souris River, Kramer, Bottineau County, ND

  3. 6. DETAIL VIEW SHOWING BASE OF LIGHT TOWER, LOOKING SOUTHEAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. DETAIL VIEW SHOWING BASE OF LIGHT TOWER, LOOKING SOUTHEAST - Monomoy Point Light Station, Approximately 3500 feet Northeast Powder Hole Pond, Monomoy National Wildlife Refuge, Chatham, Barnstable County, MA

  4. 10. DETAIL VIEW OF SPILLWAY AT DAM 83, SHOWING RIVER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. DETAIL VIEW OF SPILLWAY AT DAM 83, SHOWING RIVER COBBLE PAVING (FOREGROUND) AND WINGWALL, LOOKING EAST - Upper Souris National Wildlife Refuge, Dam 83, Souris River Basin, Foxholm, Surrey (England), ND

  5. 22. VIEW OF A SINGLE BEEHIVE COKE OVEN SHOWING THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. VIEW OF A SINGLE BEEHIVE COKE OVEN SHOWING THE INTERIOR STRUCTURE OF THE OVEN. - Tower Hill No. 2 Mine, Approximately 0.47 mile Southwest of intersection of Stone Church Road & Township Route 561, Hibbs, Fayette County, PA

  6. 9. DETAIL OF THE FAN HOUSE INTERIOR, SHOWING FAN OPENINGS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. DETAIL OF THE FAN HOUSE INTERIOR, SHOWING FAN OPENINGS. - Tower Hill No. 2 Mine, Approximately 0.47 mile Southwest of intersection of Stone Church Road & Township Route 561, Hibbs, Fayette County, PA

  7. 18. INTERIOR VIEW OF THE GENERATOR HOUSE, SHOWING CONCRETE MACHINERY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. INTERIOR VIEW OF THE GENERATOR HOUSE, SHOWING CONCRETE MACHINERY FOOTINGS, LOOKING SOUTHWEST. - Tower Hill No. 2 Mine, Approximately 0.47 mile Southwest of intersection of Stone Church Road & Township Route 561, Hibbs, Fayette County, PA

  8. 28. CROSS SECTION OF A RECTANGULAR COKE OVEN SHOWING THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. CROSS SECTION OF A RECTANGULAR COKE OVEN SHOWING THE INTERNAL STRUCTURE OF THE OVEN. - Tower Hill No. 2 Mine, Approximately 0.47 mile Southwest of intersection of Stone Church Road & Township Route 561, Hibbs, Fayette County, PA

  9. Detail unit 5, showing discharge pipe and vacuum valve on ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail unit 5, showing discharge pipe and vacuum valve on discharge side of pump - Wellton-Mohawk Irrigation System, Pumping Plant No. 1, Bounded by Gila River & Union Pacific Railroad, Wellton, Yuma County, AZ

  10. 32. VIEW LOOKING WEST SHOWING UNIT #3. VACUUM PUMP ON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. VIEW LOOKING WEST SHOWING UNIT #3. VACUUM PUMP ON LEFT, CONDENSER TURBINE ON RIGHT, JET CONDENSER IN CENTER REAR - Georgetown Steam Plant, South Warsaw Street, King County Airport, Seattle, King County, WA

  11. South side. Also showing forebay, wing for the main control ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South side. Also showing forebay, wing for the main control room, and evaporative cooling unit at left - Wellton-Mohawk Irrigation System, Pumping Plant No. 2, Bounded by Interstate 8 to south, Wellton, Yuma County, AZ

  12. 13. TOOL ROOM SHOWING W. ROBERTSON MACHINE & FOUNDRY CO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. TOOL ROOM SHOWING W. ROBERTSON MACHINE & FOUNDRY CO. NO. 5 POWER HACKSAW (FOREGROUND) AND WELLS METAL BAND SAW (BACKGROUND). VIEW SOUTHEAST - Oldman Boiler Works, Office/Machine Shop, 32 Illinois Street, Buffalo, Erie County, NY

  13. Genital Herpes Vaccine Shows Promise in Animal Trials

    MedlinePlus

    ... medlineplus.gov/news/fullstory_163137.html Genital Herpes Vaccine Shows Promise in Animal Trials Two-pronged approach ... THURSDAY, Jan. 19, 2017 (HealthDay News) -- A new vaccine for genital herpes could be nearing human clinical ...

  14. 6. EXTERIOR OF REAR (EAST END) AND NORTH SIDE SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. EXTERIOR OF REAR (EAST END) AND NORTH SIDE SHOWING ASBESTOS SIDING, BACKYARD LAWN, AND CLOTHESLINE. VIEW TO SOUTHWEST. - Bishop Creek Hydroelectric System, Plant 4, Worker Cottage, Bishop Creek, Bishop, Inyo County, CA

  15. BLDG 101, CENTRAL ENTRY/ PASSAGE SHOWING LEAD FLOOR, STEEL WALLS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BLDG 101, CENTRAL ENTRY/ PASSAGE SHOWING LEAD FLOOR, STEEL WALLS AND ASBESTOS CEILING - Naval Magazine Lualualei, Headquarters Branch, Operational Storage Building, Fifteenth Street near Kolekole Road intersection, Pearl City, Honolulu County, HI

  16. FEATURE 3, LARGE GUN POSITION, SHOWING MULTIPLE COMPARTMENTS, VIEW FACING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FEATURE 3, LARGE GUN POSITION, SHOWING MULTIPLE COMPARTMENTS, VIEW FACING SOUTH. - Naval Air Station Barbers Point, Anti-Aircraft Battery Complex-Large Gun Position, East of Coral Sea Road, northwest of Hamilton Road, Ewa, Honolulu County, HI

  17. FEATURE A. CONCRETE ANTIAIRCRAFT GUN POSITION, SHOWING CORAL RUBBLE BERM, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FEATURE A. CONCRETE ANTI-AIRCRAFT GUN POSITION, SHOWING CORAL RUBBLE BERM, VIEW FACING SOUTHEAST. - Naval Air Station Barbers Point, Battery-Anti-Aircraft Gun Position, South of Point Cruz Road & west of Coral Sea Road, Ewa, Honolulu County, HI

  18. Satellite Movie Shows Hurricane Cristobal Speeding Through North Atlantic

    NASA Video Gallery

    This animation of NOAA's GOES-East satellite imagery from August 26 through 29 shows Hurricane Cristobal changing into a post-tropical storm in the North Atlantic Ocean. Credit: NASA/NOAA GOES Project

  19. 8. Environmental view facing northwest showing pond in relationship to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Environmental view facing northwest showing pond in relationship to house - John Bly House, East side of County Road 857, just north of intersection with Quarry Run Road, Cheat Neck, Monongalia County, WV

  20. 7. William E. Barrett, Photographer, 1974. SKEWED VIEW SHOWING CHEAT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. William E. Barrett, Photographer, 1974. SKEWED VIEW SHOWING CHEAT RIVER VALLEY, REMAINS OF 1887 PIER AND c. 1900 MASONRY ARCHES. - Baltimore & Ohio Railroad, Tray Run Viaduct, Spanning Tray Run, Rowlesburg, Preston County, WV

  1. Environmental perspective facing south showing chicken house, tractor shed, and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Environmental perspective facing south showing chicken house, tractor shed, and homestead - Norris Farm, .5 mile west of County Road 857 & .25 mile east of County Road 88/1, Cheat Neck, Monongalia County, WV

  2. 2. GENERAL VIEW LOOKING EAST SHOWING SOUTH SIDE OF BRIDGE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. GENERAL VIEW LOOKING EAST SHOWING SOUTH SIDE OF BRIDGE. SHALLOW ARCH ON EXTREME RIGHT SPANS ELLIS ST. - Sudbury River Aqueduct, Echo Bridge, Spanning Charles River at Upper Newton Falls, Newton, Middlesex County, MA

  3. 20. BANKING ROOM, LOOKING SOUTH FROM NORTHWEST CORNER, SHOWING ESCALATOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. BANKING ROOM, LOOKING SOUTH FROM NORTHWEST CORNER, SHOWING ESCALATOR ENTRANCE FROM STREET ON RIGHT AND BALCONY EDGES OF TWO MEZZANINES BEYOND - Philadelphia Saving Fund Society, Twelfth & Market Streets, Philadelphia, Philadelphia County, PA

  4. 1. View southeast showing Kenyon Village from bridge over tracks ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. View southeast showing Kenyon Village from bridge over tracks on Main Street in village of Shamrock, Richmond-Charlestown, Rhode Island. - Kenyon Village, Kenyon School Road, Sherman Avenue, & Lewiston Avenue, Richmond (historical), Providence County, RI

  5. 8. 320 FOOT LEVEL, SWING ARM NINE SHOWING BACK SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. 320 FOOT LEVEL, SWING ARM NINE SHOWING BACK SIDE OF ENVIRONMENTAL CHAMBER (WHITE ROOM). WHITE ROOM MADE CONNECTION WITH CAPSULE ON LAUNCH VEHICLE. - Mobile Launcher One, Kennedy Space Center, Titusville, Brevard County, FL

  6. 34. View of pier 3, showing supporting main anchor arm ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    34. View of pier 3, showing supporting main anchor arm and cantilever arm spans, as seen from shore near pier 4, looking north - Williamstown-Marietta Bridge, Spanning Ohio River between Williamstown & Marietta, Williamstown, Wood County, WV

  7. 7. DETAIL VIEW OF ROCKER ARM, SHOWING POCKETS, LUGS, INCLINED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. DETAIL VIEW OF ROCKER ARM, SHOWING POCKETS, LUGS, INCLINED STOPPING BLOCK AT SHOREWARD END OF TRACK GIRDER - Seddon Island Scherzer Rolling Lift Bridge, Spanning Garrison Channel from Tampa to Seddon Island, Tampa, Hillsborough County, FL

  8. 22. View showing main anchor arm, as viewed from main ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View showing main anchor arm, as viewed from main cantilever arm looking south. Note upper chord eyebar arrangement. - Williamstown-Marietta Bridge, Spanning Ohio River between Williamstown & Marietta, Williamstown, Wood County, WV

  9. 6. DETAIL VIEW OF NORTHEAST END OF BRIDGE, SHOWING ROCKER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. DETAIL VIEW OF NORTHEAST END OF BRIDGE, SHOWING ROCKER ARM PORTION OF BASCULE - Seddon Island Scherzer Rolling Lift Bridge, Spanning Garrison Channel from Tampa to Seddon Island, Tampa, Hillsborough County, FL

  10. Interior view, detail view of southeast elevation to show niche ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, detail view of southeast elevation to show niche four tiers down from the east/one up from the south corner; bust is of Mendelssohn - National Park Seminary, Ballroom, Linden Lane, Silver Spring, Montgomery County, MD

  11. Interior view, close view of northwest elevation to show niche ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, close view of northwest elevation to show niche two tiers down from the west corner (niche also shown in MD-1109-F-8) - National Park Seminary, Ballroom, Linden Lane, Silver Spring, Montgomery County, MD

  12. Interior view, close view of the northwest elevation to show ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, close view of the northwest elevation to show niche four tiers down from the west corner/one from the north - National Park Seminary, Ballroom, Linden Lane, Silver Spring, Montgomery County, MD

  13. Interior view, close view of northwest elevation to show niche ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, close view of northwest elevation to show niche one tier down from the west corner; here the bust is of Beethoven - National Park Seminary, Ballroom, Linden Lane, Silver Spring, Montgomery County, MD

  14. 7. Building 7 interior, west end of building showing tier ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Building 7 interior, west end of building showing tier of skylight windows and modern equipment. View looking west. - John & James Dobson Carpet Mill (West Parcel), Building No. 7, 4041-4055 Ridge Avenue, Philadelphia, Philadelphia County, PA

  15. 10. NORTHWEST END OF WHITSETT PLANT SHOWING PIPELINES PROBABLY FOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. NORTHWEST END OF WHITSETT PLANT SHOWING PIPELINES PROBABLY FOR FIRE WATER STORAGE, LOOKING SOUTHWEST. - Whitsett Pump Plant, West side of Colorado River, north of Parker Dam, Parker Dam, San Bernardino County, CA

  16. 6. VIEW OF SPILLWAY TIMBERS AND WATER CONTROL BOX, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW OF SPILLWAY TIMBERS AND WATER CONTROL BOX, SHOWING WATER CONTROL BOX WITH LOWERED LAKE LEVEL - Three Bears Lake & Dams, Water Control Box, North of Marias Pass, East Glacier Park, Glacier County, MT

  17. 12. June 1988 INTERIOR, SOUTHWEST CORNER; SHOWING FIREFINDER (FOREGROUND), LIGHTNING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. June 1988 INTERIOR, SOUTHWEST CORNER; SHOWING FIREFINDER (FOREGROUND), LIGHTNING STOOL AND BED (BOTH TO RIGHT OF FIREFINDER) - Suntop Lookout, Forest Road 510, Mt. Baker-Snoqualmie National Forest, Greenwater, Pierce County, WA

  18. Overview of transformer platform showing three original stepup transformer (center), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Overview of transformer platform showing three original step-up transformer (center), steel switchback (right), and modern step-down transformer (foreground), view to northwest - Morony Hydroelectric Facility, Dam and Powerhouse, Morony Dam Road, Great Falls, Cascade County, MT

  19. 45. SELAH LINE, LOOKING NORTHEAST, SHOWING END OF LINE AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. SELAH LINE, LOOKING NORTHEAST, SHOWING END OF LINE AT LARSON FRUIT COMPANY - Yakima Valley Transportation Company Interurban Railroad, Connecting towns of Yakima, Selah & Wiley City, Yakima, Yakima County, WA

  20. 36. ORCHARD LINE, LOOKING SOUTH, SHOWING PACIFIC FRUIT PACKING HOUSE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. ORCHARD LINE, LOOKING SOUTH, SHOWING PACIFIC FRUIT PACKING HOUSE NEAR END OF LINE - Yakima Valley Transportation Company Interurban Railroad, Connecting towns of Yakima, Selah & Wiley City, Yakima, Yakima County, WA

  1. 2. View facing east showing the south elevation (rear) of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View facing east showing the south elevation (rear) of the garage, loading docks and rail spur, with Providence Fruit & Produce Building beyond. - Armour & Company Building, 100 Harris Avenue, Providence, Providence County, RI

  2. 19. VERTICAL VIEW, FROM DECK, SHOWING CONNECTION OF CENTER TRUSS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. VERTICAL VIEW, FROM DECK, SHOWING CONNECTION OF CENTER TRUSS TENSION BARS, DIAGONAL TENSION RODS, AND LATTICE-JOINED VERTICAL CHANNELS - Lenox Bridge, Spanning Obion River, Rural Road S8025, Lenox, Dyer County, TN

  3. 13. View South, showing the remaining pier footings for the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. View South, showing the remaining pier footings for the steam engine water tower for the Chesapeake and Ohio Railroad. - Cotton Hill Station Bridge, Spanning New River at State Route 16, Cotton Hill, Fayette County, WV

  4. 9. INTERIOR VIEW OF OPERATING HOUSE NO. 1, SHOWING DETAIL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. INTERIOR VIEW OF OPERATING HOUSE NO. 1, SHOWING DETAIL OF ELECTRICAL CONTROL PANELS, WORM WHEEL GEAR ASSEMBLY, AND NO. 1 CONTROL GATE HAND BRAKE, LOOKING WEST - Long Lake Hydroelectric Plant, Spillway Dam, Spanning Spokane River, Ford, Stevens County, WA

  5. Interior view of bedroom 2 showing louvers in former exterior ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of bedroom 2 showing louvers in former exterior window opening, facing northwest. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  6. Interior view of groundfloor servants bath showing original casement windows, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of ground-floor servants bath showing original casement windows, shower stall, and pipes at ceiling, facing southeast. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  7. General view of building in context showing residences fronting Dargue ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of building in context showing residences fronting Dargue Avenue Circle, facing south. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  8. View of southwest corner showing ell addition and carport, facing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of southwest corner showing ell addition and carport, facing northeast. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  9. Interior view of hall to bath 1 showing typical doors ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of hall to bath 1 showing typical doors and attic scuttle, facing east. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  10. Oblique view of northwest corner showing screened openings at ground ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of northwest corner showing screened openings at ground floor and mission scrolls, facing east. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  11. Interior view of groundfloor porch showing exposed concrete floor slab ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of ground-floor porch showing exposed concrete floor slab system, facing west. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  12. Interior view of bath 1 showing original tub and shower ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of bath 1 showing original tub and shower stall, facing southwest. - Albrook Air Force Station, Field Officer's Quarters, West side of Dargue Avenue Circle, Balboa, Former Panama Canal Zone, CZ

  13. 12. INTERIOR VIEW OF TRANSFORMER BUILDING, HIGHLINE PUMP PLANT, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. INTERIOR VIEW OF TRANSFORMER BUILDING, HIGHLINE PUMP PLANT, SHOWING NEW SWITCHES AND METERS, December 3, 1952 - Highline Canal & Pumping Station, South side of Salt River between Tempe, Phoenix & Mesa, Tempe, Maricopa County, AZ

  14. 8. Close view looking from the east to show cistern ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Close view looking from the east to show cistern and electric meter - Piece Sur Piece Building (House), On dirt road off of Highway 494, about 1 1/2 miles Northwest of Bermuda, Bermuda, Natchitoches Parish, LA

  15. 49. Photocopy of photograph, AERIAL PHOTOGRAPH SHOWING VIEW OF CNJ ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    49. Photocopy of photograph, AERIAL PHOTOGRAPH SHOWING VIEW OF CNJ BRIDGE PRIOR TO DEVELOPMENT OF PORT ELIZABETH - Central Railroad of New Jersey, Newark Bay Lift Bridge, Spanning Newark Bay, Newark, Essex County, NJ

  16. 39. Historic photograph, photographer unknown, c. 1944. VIEW SHOWING SHEEP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. Historic photograph, photographer unknown, c. 1944. VIEW SHOWING SHEEP CROSSING BRIDGE, LOOKING WEST FROM CORRAL AT EAST APPROACH TO WALKWAY. - Verde River Sheep Bridge, Spanning Verde River (Tonto National Forest), Cave Creek, Maricopa County, AZ

  17. 34. Historic photograph, photographer unknown, October 10, 1944. VIEW SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    34. Historic photograph, photographer unknown, October 10, 1944. VIEW SHOWING HORSESHOE DAM UNDER CONSTRUCTION, LOOKING WEST. ONLY THE BRIDGE TOWERS REMAIN. - Verde River Sheep Bridge, Spanning Verde River (Tonto National Forest), Cave Creek, Maricopa County, AZ

  18. 3. AERIAL VIEW SHOWING THE ENTIRE BRIDGE FROM EAST CABLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AERIAL VIEW SHOWING THE ENTIRE BRIDGE FROM EAST CABLE ANCHORAGE (EXTREME LEFT) TO WEST CABLE ANCHORAGE (UPPER RIGHT CORNER). March 1987. - Verde River Sheep Bridge, Spanning Verde River (Tonto National Forest), Cave Creek, Maricopa County, AZ

  19. 38. Historic photograph, photographer unknown, c. 1944. VIEW SHOWING BURROS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. Historic photograph, photographer unknown, c. 1944. VIEW SHOWING BURROS (OR MULES) CROSSING BRIDGE, LOOKING NORTHEAST. - Verde River Sheep Bridge, Spanning Verde River (Tonto National Forest), Cave Creek, Maricopa County, AZ

  20. 90. STARBOARD CATAPULT AFT LOOKING FORWARD SHOWING CROSSHEAD, RAILS, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    90. STARBOARD CATAPULT - AFT LOOKING FORWARD SHOWING CROSSHEAD, RAILS, RAM CYLINDER, RAM (ON DECK) AND RETRACTING ENGINE SHEAVE IN THE OVERHEAD. - U.S.S. HORNET, Puget Sound Naval Shipyard, Sinclair Inlet, Bremerton, Kitsap County, WA

  1. 6. DETAIL VIEW OF ENTRANCE GATES, SHOWING IRON GATE, STONE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. DETAIL VIEW OF ENTRANCE GATES, SHOWING IRON GATE, STONE WORK, AND GATE STOP FROM SOUTHEAST OF NORTHWEST ELEMENTS. - William Enston Home, Entrance Gate, 900 King Street, Charleston, Charleston County, SC

  2. 11. UNDERSIDE FROM SOUTH ABUTMENT SHOWING FLOOR SYSTEM, WALKWAY, AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. UNDERSIDE FROM SOUTH ABUTMENT SHOWING FLOOR SYSTEM, WALKWAY, AND FIRST PIER. LOOKING NORTH. - Rue Road Bridge, Rue Road, spanning Matchaponix Brook, .35 mile east of intersection with Route 613, Jamesburg, Middlesex County, NJ

  3. 12. Exterior detail view of roof structure at eave, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Exterior detail view of roof structure at eave, showing exposed rafter tails, skip sheathing and gutter - American Railway Express Company Freight Building, 1060 Northeast Division Street, Bend, Deschutes County, OR

  4. GPM Shows Large Rainfall Totals from Typhoon Dujuan

    NASA Video Gallery

    GPM showed that Taiwan's heaviest rainfall totals of over 275 mm (10.8 inches) were located along the coast south of where Typhoon Dujuan made landfall. The highest rainfall totals were found over ...

  5. East side, showing ruin of brownstone column capital that originally ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    East side, showing ruin of brownstone column capital that originally supported the east side portico, a feature that was destroyed in the 1886 earthquake - William Ravenel House, 13 East Battery Street, Charleston, Charleston County, SC

  6. 53. INTERIOR VIEW LOOKING NORTH NORTHEAST SHOWING THE REMAINS OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    53. INTERIOR VIEW LOOKING NORTH NORTHEAST SHOWING THE REMAINS OF A WOODEN SETTLING BOX IN THE BACKGROUND RIGHT. AMALGAMATING PANS IN THE FOREGROUND. - Standard Gold Mill, East of Bodie Creek, Northeast of Bodie, Bodie, Mono County, CA

  7. CRIB DAM, LOOKING ALONG DAM FROM WEST ABUTMENT, SHOWING PLANK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CRIB DAM, LOOKING ALONG DAM FROM WEST ABUTMENT, SHOWING PLANK SHEATHING IN FOREGROUND. VIEW TO EAST - Kachess Dam, 1904 Cascade Canal Company Crib Dam, Kachess River, 1.5 miles north of Interstate 90, Easton, Kittitas County, WA

  8. View of submerged remains of Read Sawmill, showing floor boards, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of submerged remains of Read Sawmill, showing floor boards, cross beams and notches for wall post beams. - Silas C. Read Sawmill, Outlet of Maxwell Lake near North Range Road, Fort Gordon, Richmond County, GA

  9. View of double floor boards with mortises cross beams, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of double floor boards with mortises cross beams, showing spikes and flooring nails (Lower board layer exposed) - Silas C. Read Sawmill, Outlet of Maxwell Lake near North Range Road, Fort Gordon, Richmond County, GA

  10. View of Northwest corner of the Merry Generator House showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of Northwest corner of the Merry Generator House showing floor boards and cross beams. Shifting of structure evident in staggered wall board profile. - Arthur Holmes Merry Generator House, Signal Lake North of Range Road, Fort Gordon, Richmond County, GA

  11. View of submerged remains of Read Sawmill, showing floor boards, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of submerged remains of Read Sawmill, showing floor boards, wall boards, tenoned uprights and mortised sill beams. - Silas C. Read Sawmill, Outlet of Maxwell Lake near North Range Road, Fort Gordon, Richmond County, GA

  12. 16. MASONRY DETAIL NO. 2, NORTH TRAINING WALL, SHOWING THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. MASONRY DETAIL NO. 2, NORTH TRAINING WALL, SHOWING THE RUBBLE CORE WHERE THE FACING STONES HAVE BEEN REMOVED. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

  13. 8. WEST END OF NORTH TRAINING WALL, LOOKING NORTHWEST, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. WEST END OF NORTH TRAINING WALL, LOOKING NORTHWEST, SHOWING THE LIGHTHOUSE SITE OFFSHORE, AND THE UNIDENTIFIED STRUCTURE FOUNDATIONS ONSHORE AT FAR RIGHT. - Oakland Harbor Training Walls, Mouth of Federal Channel to Inner Harbor, Oakland, Alameda County, CA

  14. 10. UPSTREAM SIDE OF UPPER MITER GATES SHOWING STOWED LEFT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. UPSTREAM SIDE OF UPPER MITER GATES SHOWING STOWED LEFT WING OF UPPER GUARD GATE (FAR LEFT). VIEW TO NORTHWEST. - Starved Rock Locks & Dam, Illinois Waterway River mile 231, Peru, La Salle County, IL

  15. 19. STAIRWAY TO TAINTER GATE SECTION OF SPILLWAY, SHOWING STEAM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. STAIRWAY TO TAINTER GATE SECTION OF SPILLWAY, SHOWING STEAM PIPES EMERGING FROM BOILERHOUSE (RIGHT) AND CONCRETE TAINTER GATE COUNTER WEIGHTS (BACKGROUND RIGHT). VIEW TO SOUTH. - Starved Rock Locks & Dam, Illinois Waterway River mile 231, Peru, La Salle County, IL

  16. 3. GENERAL VIEW SHOWING (FROM LEFT TO RIGHT) LOCKMASTER'S HOUSE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. GENERAL VIEW SHOWING (FROM LEFT TO RIGHT) LOCKMASTER'S HOUSE, VISITORS CENTER, AND NEW GARAGE. VIEW TO SOUTHWEST. - Starved Rock Locks & Dam, Illinois Waterway River mile 231, Peru, La Salle County, IL

  17. 1. FRONT (SOUTH) SIDE OF LOCKMASTER'S HOUSE SHOWING PORTION OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. FRONT (SOUTH) SIDE OF LOCKMASTER'S HOUSE SHOWING PORTION OF LOCK CHAMBER IN FOREGROUND. VIEW TO NORTH. - Starved Rock Locks & Dam, Lockmaster's House, Illinois Waterway River mile 231, Peru, La Salle County, IL

  18. 23. VIEW, FROM EAST, SHOWING DIVING AND MAIN POOLS AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. VIEW, FROM EAST, SHOWING DIVING AND MAIN POOLS AND WEST ELEVATION OF OFFICE AND FIRST AID BUILDING - Glen Echo Park, Crystal Swimming Pool, 7300 McArthur Boulevard, Glen Echo, Montgomery County, MD

  19. 5. South wall of Gas House showing connection to bridge. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. South wall of Gas House showing connection to bridge. - Delaware, Lackawanna & Western Railroad, Scranton Yards, Gas House, 100 block of South Washington Avenue, west side, Scranton, Lackawanna County, PA

  20. 32. Otter Lake Dam. View from downstream show how the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. Otter Lake Dam. View from downstream show how the dam blends into its environment. Looking east-northeast. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  1. 58. VIEW SHOWING GRAFFITI IN STAIRWELL INSIDE 'CATFISH' SILO Everett ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    58. VIEW SHOWING GRAFFITI IN STAIRWELL INSIDE 'CATFISH' SILO Everett Weinreb, photographer, March 1988 - Mount Gleason Nike Missile Site, Angeles National Forest, South of Soledad Canyon, Sylmar, Los Angeles County, CA

  2. 23. Surrender interview site, showing Pemberton Avenue concrete slab road ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Surrender interview site, showing Pemberton Avenue concrete slab road type with gutter (asphalt construction typical on Union and Confederate Avenues), view to the sw. - Vicksburg National Military Park Roads & Bridges, Vicksburg, Warren County, MS

  3. 1. EAST SIDE SHOWING LOW CINDERBLOCK WALL AND ASPHALTPAVED PARKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. EAST SIDE SHOWING LOW CINDER-BLOCK WALL AND ASPHALT-PAVED PARKING LOT FOR NEW CONTROL BUILDING. VIEW TO NORTHWEST. - Bishop Creek Hydroelectric System, Control Station, Hydrographer's Office, Bishop Creek, Bishop, Inyo County, CA

  4. 12. VIEW NORTH, ACROSS DECK CENTER AREA SHOWING ASPHALT AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. VIEW NORTH, ACROSS DECK CENTER AREA SHOWING ASPHALT AND NORTH SIDE GUARD WALL - Route 1 Extension, South Street Viaduct, Spanning Conrail & Wheeler Point Road at South Street, Newark, Essex County, NJ

  5. 9. VIEW NORTH, ACROSS DECK AT EAST SIDE SHOWING GRANITE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW NORTH, ACROSS DECK AT EAST SIDE SHOWING GRANITE BLOCK PAVING, EXPANSION JOINT AND NORTH SIDE PIPE RAILING - Route 1 Extension, South Street Viaduct, Spanning Conrail & Wheeler Point Road at South Street, Newark, Essex County, NJ

  6. 16. PIER CAP DETAIL, SHOWING EXPANSION AND FIXED BEARING SHOES, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. PIER CAP DETAIL, SHOWING EXPANSION AND FIXED BEARING SHOES, BOTTOM CHORD / END POST CONNECTION AND CANTILEVERED SIDEWALK. VIEW TO WEST. - Holbrook Bridge, Spanning Little Colorado River at AZ 77, Holbrook, Navajo County, AZ

  7. 13. Interior, Hangar 1301, showing bottom of a truss, steel ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Interior, Hangar 1301, showing bottom of a truss, steel hinge point and expansion joint, and concrete buttress, looking north northwest - Dover Air Force Base, Hangar No. 1301, Dover, Kent County, DE

  8. 13. VIEW LOOKING INSIDE SILO, SHOWING ELEVATOR (ON LEFT) AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW LOOKING INSIDE SILO, SHOWING ELEVATOR (ON LEFT) AND AIR CONDITIONING UNIT Everett Weinreb, photographer, April 1988 - Los Pinetos Nike Missile Site, Santa Clara Road, Los Angeles National Forest, Sylmar, Los Angeles County, CA

  9. 9. Roof. A view looking north, showing the elevator bulkheads ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Roof. A view looking north, showing the elevator bulkheads and the retrofit air-conditioning compressors and air handlers. - John T. Beasley Building, 632 Cherry Street (between Sixth & Seventh Streets), Terre Haute, Vigo County, IN

  10. 32. Coffee bean sluiceway on ground floor showing chute bringing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. Coffee bean sluiceway on ground floor showing chute bringing beans from first floor hopper. HAER PR, 6-MAGU, 1B-17 - Hacienda Buena Vista, PR Route 10 (Ponce to Arecibo), Magueyes, Ponce Municipio, PR

  11. 7. DETAIL VIEW OF PIN CONNECTION, NORTH WEB, SHOWING FLOOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. DETAIL VIEW OF PIN CONNECTION, NORTH WEB, SHOWING FLOOR BEANS, STRINGERS, LATTICE BRACING, EYEBARS AND DECKING, LOOKING SOUTH - Four Mile Bridge, Spanning Elk River on County Road 42, Steamboat Springs, Routt County, CO

  12. 8. DETAIL: GENERATOR FLOOR DIABLO POWERHOUSE SHOWING BUTTERFLY VALVE CONTROL, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. DETAIL: GENERATOR FLOOR DIABLO POWERHOUSE SHOWING BUTTERFLY VALVE CONTROL, MOSAIC TILE FLOOR, AS SEEN FROM VISITORS GALLERY, 1989. - Skagit Power Development, Diablo Powerhouse, On Skagit River, 6.1 miles upstream from Newhalem, Newhalem, Whatcom County, WA

  13. Detail of exciter turbine showing shaft, scroll case, servomotor and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of exciter turbine showing shaft, scroll case, servo-motor and operating ring (left foreground) and hand wheel for butterfly valve (right background) - Morony Hydroelectric Facility, Dam and Powerhouse, Morony Dam Road, Great Falls, Cascade County, MT

  14. 6. Photocopy of drawing showing pile bridge construction on Erie ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopy of drawing showing pile bridge construction on Erie Railway in 1841. Original illustration in DeGolyer Collection, Dallas, Texas. - Erie Railway, New Jersey, New York, Pennsylvania, Deposit, Broome County, NY

  15. 1. Photocopy of drawing showing pile superstructure of early railroad ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of drawing showing pile superstructure of early railroad track construction. Original illustration in Degolyer Collection, Dallas, Texas - Erie Railway, New Jersey, New York, Pennsylvania, Deposit, Broome County, NY

  16. Interior view of coffee processing structure No. 1, showing concrete ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of coffee processing structure No. 1, showing concrete reservoirs on floor, view towards the west - Finca Silem, Coffee Processing Structure No. 1, Highway 139, Kilometer 9.3, Maraguez, Ponce Municipio, PR

  17. 9. INTERIOR, VIEW SHOWING MAIN STAIRWAY AND ENTRY HALL THROUGH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. INTERIOR, VIEW SHOWING MAIN STAIRWAY AND ENTRY HALL THROUGH ENTRY INTO PARLOR. TAKEN FROM SOUTHWEST CORNER OF PARLOR, LOOKING NORTHWEST - Farquhar House, 1601 Sandy Spring Road (Route 108), Sandy Spring, Montgomery County, MD

  18. EPA Survey Shows $271 Billion Needed for Nations Wastewater Infrastructure

    EPA Pesticide Factsheets

    WASHINGTON - The U.S. Environmental Protection Agency (EPA) today released a survey showing that $271 billion is needed to maintain and improve the nation's wastewater infrastructure, including the pipes that carry wastewater to treatment plants, th

  19. 5. View showing Crooked River High Bridge in background and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. View showing Crooked River High Bridge in background and Ralph Modjeski railroad bridge in foreground - Crooked River High Bridge, Spanning Crooked River Gorge at Dalles-California Highway, Terrebonne, Deschutes County, OR

  20. 9. MAIN (WEST) ELEVATION, DETAIL OF DOORWAY, SHOWING FRONTISPIECE OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. MAIN (WEST) ELEVATION, DETAIL OF DOORWAY, SHOWING FRONTISPIECE OF COMPOSITE ATTACHED COLUMNS, SWAN'S NECK PEDIMENT WITH PINEAPPLE, EGG-AND-DART RELIEF AROUND ARCH - Mt. Harmon Plantation at World's End, Earleville, Cecil County, MD

  1. GOES-West Shows U.S. West's Record Rainfall

    NASA Video Gallery

    A new time-lapse animation of data from NOAA's GOES-West satellite provides a good picture of why the U.S. West Coast continues to experience record rainfall. The new animation shows the movement o...

  2. 2. Looking West, showing a view of the two through ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Looking West, showing a view of the two through trusses and only two of the pony trusses from the downstream side of the structure. - Weidemeyer Bridge, Spanning Thomes Creek at Rawson Road, Corning, Tehama County, CA

  3. 2. View of NE elevation of corn crib showing doubletrack ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. View of NE elevation of corn crib showing double-track rail system leading to upper level. - Laurel Valley Sugar Plantation, Corn Crib, 2 miles South of Thibodaux on State Route 308, Thibodaux, Lafourche Parish, LA

  4. 3. Interior view of corn crib showing heavytimber framing, railed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Interior view of corn crib showing heavy-timber framing, railed trackway and corn car at upper level. - Laurel Valley Sugar Plantation, Corn Crib, 2 miles South of Thibodaux on State Route 308, Thibodaux, Lafourche Parish, LA

  5. 11. DETAIL VIEW OF GENERATOR NO. 1, GENERATOR ROOM, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. DETAIL VIEW OF GENERATOR NO. 1, GENERATOR ROOM, SHOWING GRAVITY LUBRICATING OIL BOX ABOVE GENERATOR - Nine Mile Hydroelectric Development, Powerhouse, State Highway 291 along Spokane River, Nine Mile Falls, Spokane County, WA

  6. 30. Photocopy of lithograph showing Empire Stores at corner (Baker, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. Photocopy of lithograph showing Empire Stores at corner (Baker, Ostheimer and Co.) from Everts, Ensign & Everts, Combination Atlas Map of Erie County, 1876 - Empire Stores, 501-505 State Street, Erie, Erie County, PA

  7. Interior view, detail to show the ornate bronze doors to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, detail to show the ornate bronze doors to the entrance lobby elevators - United States Department of Commerce, Bounded by Fourteenth, Fifteenth, and E streets and Constitution Avenue, Washington, District of Columbia, DC

  8. Interior view, law library closer view to show painted details ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view, law library closer view to show painted details on the groin vaults - United States Department of Commerce, Bounded by Fourteenth, Fifteenth, and E streets and Constitution Avenue, Washington, District of Columbia, DC

  9. Detail view to show richness of materials such as the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view to show richness of materials such as the Fourteenth Street vestibule with its gilded groin-vaulted ceiling - United States Department of Commerce, Bounded by Fourteenth, Fifteenth, and E streets and Constitution Avenue, Washington, District of Columbia, DC

  10. 129. FULL AERIAL VIEW SHOWING FORWARD PORT QUARTER, ENTERING PEARL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    129. FULL AERIAL VIEW SHOWING FORWARD PORT QUARTER, ENTERING PEARL HARBOR AFTER APOLLO 11 RECOVERY. 26 JULY 1969. (NATIONAL ARCHIVES NO. 428-KN-18090) - U.S.S. HORNET, Puget Sound Naval Shipyard, Sinclair Inlet, Bremerton, Kitsap County, WA

  11. 9. VIEW SHOWING TRUSSES FROM DECK WITH 4' RANGE POLE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW SHOWING TRUSSES FROM DECK WITH 4' RANGE POLE AT SECOND VERTICAL POST ON SOUTH SIDE, LOOKING WEST - White River Bridge, Spanning White River at U.S. Highway 70, De Valls Bluff, Prairie County, AR

  12. 8. LOOKING EAST FROM TOP OF WATER TOWER: VIEW SHOWS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. LOOKING EAST FROM TOP OF WATER TOWER: VIEW SHOWS BUILDING #626 AND PORTION OF QUADRANGLE - Fort Sam Houston, San Antonio Depot, Water-Watch Tower, Grayson Street & New Braunfels Avenue, San Antonio, Bexar County, TX

  13. General view of the archaeological site showing excavation and revealing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of the archaeological site showing excavation and revealing the steps leading down into the eighteenth-century burial vault - Harry Buck House, North of Main Street (14800 Governor Oden Bowie Drive), Upper Marlboro, Prince George's County, MD

  14. Detail view of stone entrance gate pylon showing carved site ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of stone entrance gate pylon showing carved site name and Great Seal of the United States. View looking northeast. - Flanders Field American Cemetery & Memorial, Wortegemseweg 117, Waregem, West Flanders (Belgium)

  15. 8. IRON MOUNTAIN SHAFT ROOM TO UNIT #5 SHOWING TYPICAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. IRON MOUNTAIN SHAFT ROOM TO UNIT #5 SHOWING TYPICAL ARRANGEMENT OF SHAFT AND PUMP IN COLORADO RIVER AQUEDUCT PUMPHOUSES. - Iron Mountain Pump Plant, South of Danby Lake, north of Routes 62 & 177 junction, Rice, San Bernardino County, CA

  16. 19. Interior view of eastern segment of Roundhouse showing clerestory ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Interior view of eastern segment of Roundhouse showing clerestory lighting and locomotive smoke flues. - Central of Georgia Railway, Savannah Repair Shops & Terminal Facilities, Roundhouse, Site Bounded by West Broad, Jones, West Boundary & Hull, Savannah, Chatham County, GA

  17. 12. VIEW OF ELEVATOR MACHINE ROOM, SHOWING LOW CABINET TEAT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. VIEW OF ELEVATOR MACHINE ROOM, SHOWING LOW CABINET TEAT HOUSES HYDRAULIC CYLINDER AND CLOSET AT LEFT THAT HOUSES OPERATING VALVE, LOOKING NORTHEAST - 72 Marlborough Street, Residential Hydraulic Elevator, Boston, Suffolk County, MA

  18. 22. VIEW SOUTH, INTERIOR OF SOUTH PIT, SHOWING FINAL STEP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. VIEW SOUTH, INTERIOR OF SOUTH PIT, SHOWING FINAL STEP IN GEARING THAT DRIVES OPERATING WHEEL, WITH HYDRAULIC SHAFT BRAKE - Mystic River Bridge, Spanning Mystic River at U.S. Route 1, Groton, New London County, CT

  19. 4. VENTILATION FAN SHOWING RELATIVE POSITION IN THE AIR TUNNEL. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VENTILATION FAN SHOWING RELATIVE POSITION IN THE AIR TUNNEL. - Hot Springs National Park, Bathhouse Row, Ozark Bathhouse: Mechanical & Piping Systems, State Highway 7, 1 mile north of U.S. Highway 70, Hot Springs, Garland County, AR

  20. 3. General view showing rear of looking glass aircraft. View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. General view showing rear of looking glass aircraft. View to north. - Offutt Air Force Base, Looking Glass Airborne Command Post, Looking Glass Aircraft, On Operational Apron covering northeast half of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  1. 4. View showing underside of wing, looking glass aircraft. View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. View showing underside of wing, looking glass aircraft. View to north. - Offutt Air Force Base, Looking Glass Airborne Command Post, Looking Glass Aircraft, On Operational Apron covering northeast half of Project Looking Glass Historic District, Bellevue, Sarpy County, NE

  2. 18. INTERIOR OF KITCHEN NO. 1 SHOWING STAINED CABINETRY ON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. INTERIOR OF KITCHEN NO. 1 SHOWING STAINED CABINETRY ON OPPOSITE WALL FROM PAINTED CABINETS. VIEW TO NORTHEAST. - Bishop Creek Hydroelectric System, Plant 6, Cashbaugh-Kilpatrick House, Bishop Creek, Bishop, Inyo County, CA

  3. INTERIOR VIEW OF THE LANAI. SHOWING THE ORIGINAL STAINED CONCRETE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF THE LANAI. SHOWING THE ORIGINAL STAINED CONCRETE FLOOR WITH INCISED LINES, AND HINGED DOOR TO GARAGE WITH VERTICAL BOARD PANELING (BACKGROUND). VIEW FACING NORTHWEST. - Hickam Field, Officers' Housing Type J, 701 Beard Street, Honolulu, Honolulu County, HI

  4. INTERIOR VIEW OF ENTRY. SHOWING THE STAINED CONCRETE FLOOR AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF ENTRY. SHOWING THE STAINED CONCRETE FLOOR AND WINDOW WITH DIAMOND PATTERN MUNTINS. VIEW FACING NORTHWEST. - Hickam Field, Officers' Housing Type F, 602 Beard Avenue, Honolulu, Honolulu County, HI

  5. DETAIL OF THE FRONT PORCH SHOWING THE SQUARE COLUMN, COVED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL OF THE FRONT PORCH SHOWING THE SQUARE COLUMN, COVED CEILING, AND STAINED CONCRETE FLOOR. VIEW FACING NORTHWEST. - Hickam Field, Officers' Housing Type H, 208 Sixth Street, Honolulu, Honolulu County, HI

  6. 4. GENERAL VIEW, LOOKING NORTH, SHOWING BRIDGE PARAPETS AND SETTING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. GENERAL VIEW, LOOKING NORTH, SHOWING BRIDGE PARAPETS AND SETTING (SCALE ROD IS MEASURED IN FEET) - Spring Lake Bridge, Spanning Bob Barnes Branch at County Road No. 36D, Belleville, Yell County, AR

  7. 6. DETAIL VIEW, LOOKING NORTH, SHOWING DRAINAGE HOLE AT BASE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. DETAIL VIEW, LOOKING NORTH, SHOWING DRAINAGE HOLE AT BASE OF THE INTERIOR NORTH WALL OF THE NORTH ARCH - Spring Lake Bridge, Spanning Bob Barnes Branch at County Road No. 36D, Belleville, Yell County, AR

  8. Overview of interior showing turbine (center) and transformer (left). View ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Overview of interior showing turbine (center) and transformer (left). View to north-northwest - Flint Creek Hydroelectric Project, Powerhouse, Approximately 3 miles southeast of Porters Corner on Powerhouse Road, Philipsburg, Granite County, MT

  9. 4. DETAIL ALONG WEST SIDE, SHOWING EXTERIOR STAIRWAY, BUILDING NO. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. DETAIL ALONG WEST SIDE, SHOWING EXTERIOR STAIRWAY, BUILDING NO. 1 IN THE CENTER DISTANCE, AND ONE OF THE BENDING SHOPS AT RIGHT. - United Engineering Company Shipyard, Engineering Building, 2900 Main Street, Alameda, Alameda County, CA

  10. 23. VIEW NORTHWEST ON VIRGINIA AVENUE AXIS SHOWING NEW JERSEY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. VIEW NORTHWEST ON VIRGINIA AVENUE AXIS SHOWING NEW JERSEY AVENUE OVERPASS AND WASHINGTON MONUMENT. TAKEN FROM UNDERNEATH THE SOUTHEAST/SOUTHWEST FREEWAY. - Virginia Avenue, Washington, District of Columbia, DC

  11. 22. VIEW SOUTHWEST ON VIRGINIA AVENUE AXIS SHOWING SOUTH SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. VIEW SOUTHWEST ON VIRGINIA AVENUE AXIS SHOWING SOUTH SIDE OF GARFIELD PARK, NARROW ROADWAY, RAILROAD TRACKS ENTERING THE TUNNEL, AND THE SOUTHEAST/SOUTHWEST FREEWAY. TAKEN FROM THE NEW JERSEY AVENUE OVERPASS. - Virginia Avenue, Washington, District of Columbia, DC

  12. 2. GENERAL VIEW LOOKING NORTHEAST, SHOWING COKE MACHINE (CENTER), INTERMEDIATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. GENERAL VIEW LOOKING NORTHEAST, SHOWING COKE MACHINE (CENTER), INTERMEDIATE TIPPLE (RIGHT), AND OVENS - Shoaf Mine & Coke Works, East side of Shoaf, off Township Route 472, Shoaf, Fayette County, PA

  13. 1. GENERAL VIEW OF COKE WORKS LOOKING WEST, SHOWING OVENS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. GENERAL VIEW OF COKE WORKS LOOKING WEST, SHOWING OVENS IN FOREGROUND, LARRY CAR TIPPLE TO THE RIGHT, AND COAL TIPPLE IN CENTERGROUND - Lucernemines Coke Works, 0.2 mile East of Lucerne, Lucerne Mines, Indiana County, PA

  14. 30. Perimeter acquisition radar building room #318, showing radar control. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. Perimeter acquisition radar building room #318, showing radar control. Console and line printers - Stanley R. Mickelsen Safeguard Complex, Perimeter Acquisition Radar Building, Limited Access Area, between Limited Access Patrol Road & Service Road A, Nekoma, Cavalier County, ND

  15. 20. Third approach span, comparing pier types and showing guardrail ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Third approach span, comparing pier types and showing guardrail and connection to arch spring point, looking east - U.S. Route 1 Nottoway River Bridge, U.S. Route 1 spanning Nottoway River, McKenney, Dinwiddie County, VA

  16. 3. Historic American Buildings Survey February, 1953 LOOKING EAST, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey February, 1953 LOOKING EAST, SHOWING A GROUP OF HAGERSTOWN HIGH SCHOOL STUDENTS IN THE PROCESS OF UNCOVERING THE ADDITIONAL FOUNDATION - Jonathan Hager House (Foundation), Hagerstown, Washington County, MD

  17. 20. VIEW, LOOKING NORTH FROM BOSTON, SHOWING RAILING, PEDESTRIAN STAIR, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. VIEW, LOOKING NORTH FROM BOSTON, SHOWING RAILING, PEDESTRIAN STAIR, AND '10 SMOOT' MARKER (see data pages) - Harvard Bridge, Spanning Charles River at Massachusetts Avenue, Boston, Suffolk County, MA

  18. 4. VIEW SHOWING EXCAVATION IN ARIZONA CANAL, 8 MILES NORTHEAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW SHOWING EXCAVATION IN ARIZONA CANAL, 8 MILES NORTHEAST OF PHOENIX. NOTE MEN DRILLING AND EXCAVATING IN OPERATION; CAMELBACK MOUNTAIN IN THE DISTANCE Photographer: Walter J. Lubken. No date - Arizona Canal, North of Salt River, Phoenix, Maricopa County, AZ

  19. EXTERIOR ELEVATION, LOOKING SOUTH, ALSO SHOWING THE NORFOLK SOUTHERN STEAM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    EXTERIOR ELEVATION, LOOKING SOUTH, ALSO SHOWING THE NORFOLK SOUTHERN STEAM RESTORATION FLOOR INTERIOR WITH A DRILL PRESS (LEFT) AND BORING MILL (RIGHT). - Norfolk & Southern Steam Locomotive No. 1218, Norris Yards, East of Ruffner Road, Irondale, Jefferson County, AL

  20. Detail view of Fanno Creek trestle, showing trestle substructure, view ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of Fanno Creek trestle, showing trestle substructure, view looking north - Oregon Electric Railroad, Fanno Creek Trestle, Garden Home to Wilsonville Segment, Milepost 34.7, Garden Home, Washington County, OR

  1. 5. VIEW NORTHWEST SHOWING AQUEDUCT PRISM. NOTE INTERIOR STONE WORK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. VIEW NORTHWEST SHOWING AQUEDUCT PRISM. NOTE INTERIOR STONE WORK OF THE PARAPET WALL AND REMAINS OF 1920 TIMBER AND CONCRETE FLOORING SYSTEM. - Chesapeake & Ohio Canal, Conococheague Creek Aqueduct, Milepost 99.80, Williamsport, Washington County, MD

  2. 17. SOUTH SIDE, EAST HALF, PART 3, SHOWING THE EASTERN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. SOUTH SIDE, EAST HALF, PART 3, SHOWING THE EASTERN PART OF THE OFFICES AND BUILDING NO. 12 IN THE DISTANCE BEYOND THE EAST END. - United Engineering Company Shipyard, Inspection & Repair Shops, 2900 Main Street, Alameda, Alameda County, CA

  3. Interior view of living and dining areas, showing their separation ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of living and dining areas, showing their separation by medio punto, view towards the north, northeast - Pou Coffee Processing Structure, Casa No. 2, Highway 139, Kilometer 12, Maraguez, Ponce Municipio, PR

  4. 3. August, 1971. VIEW ALONG CANAL SHOWING BORDER PATH AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. August, 1971. VIEW ALONG CANAL SHOWING BORDER PATH AND BRIDGE FOR INSPECTION - ABOUT ONE MILE FROM CANAL HEAD. - Hurricane Irrigation Canal, State Route 15 Vicinity, Hurricane, Washington County, UT

  5. VIEW FROM PLATFORM FACING ROOMS AND SHOWING TRENCH PLATES. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW FROM PLATFORM FACING ROOMS AND SHOWING TRENCH PLATES. - U.S. Naval Base, Pearl Harbor, Drum & Can Loading Facility, South of Arizona Street near Kamehameha Highway, Pearl City, Honolulu County, HI

  6. 4. GENERAL VIEW SHOWING EARTHEN BERM AROUND STRUCTURE. NOTE INSTRUMENTATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. GENERAL VIEW SHOWING EARTHEN BERM AROUND STRUCTURE. NOTE INSTRUMENTATION TRENCH IN FOREGROUND RIGHT; VIEW TO WEST. - Cape Canaveral Air Station, Launch Complex 17, Facility 28401, East end of Lighthouse Road, Cape Canaveral, Brevard County, FL

  7. 6. VIEW LOOKING WEST FROM BRIDGE DECK, SHOWING TOP LATERAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW LOOKING WEST FROM BRIDGE DECK, SHOWING TOP LATERAL BRACING, MAIN TIE AND COUNTERS, SWAY STRUTS AND BRACING, AND INTERMEDIATE POSTS - Hart County Bridge, State Route 181, spanning Savannah River, Hartwell, Hart County, GA

  8. 4. DETAIL VIEW OF EAST (FRONT) ELEVATION, WITH SCALE, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. DETAIL VIEW OF EAST (FRONT) ELEVATION, WITH SCALE, SHOWING ENTRY, WALL FINISH, VARIOUS WINDOWS AND CORNICE - Sacred Heart Church at Whitemarsh, 16101 Annapolis Road, Bowie, Prince George's County, MD

  9. 7. Contextual view to eastnortheast showing downstream (west) side of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Contextual view to east-northeast showing downstream (west) side of bridge in setting, depicting dense riparian nature of area. - Stanislaus River Bridge, Atchison, Topeka & Santa Fe Railway at Stanislaus River, Riverbank, Stanislaus County, CA

  10. 6. HISTORIC AMERICAN BUILDINGS SURVEY, INTERIOR SHOWING ORIGINAL GRANITE COLUMNS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. HISTORIC AMERICAN BUILDINGS SURVEY, INTERIOR SHOWING ORIGINAL GRANITE COLUMNS AND COLUMN BRICKFACED AFTER THE GREAT FIRE 1904 - Old U.S. Appraisers Stores, Gay & Lombard Streets, Baltimore, Independent City, MD

  11. 1. VIEW INTO SHAFT ON FIRST FLOOR, SHOWING OPERATING ROPE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW INTO SHAFT ON FIRST FLOOR, SHOWING OPERATING ROPE TO RIGHT OF DOOR JAMB, OPENING AT LEFT TO FRONT PARLOR, LOOKING SOUTHEAST - 72 Marlborough Street, Residential Hydraulic Elevator, Boston, Suffolk County, MA

  12. Detail, east truss of south span, showing railing, vertical UL, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail, east truss of south span, showing railing, vertical U-L, diagonal eyebar U-L with turnbuckle - Castle Garden Bridge, Township Route 343 over Bennetts Branch of Sinnemahoning Creek, Driftwood, Cameron County, PA

  13. 1. General view of stockyards from livestock exchange building showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. General view of stockyards from livestock exchange building showing (l-r) cattle pens and Buckingham Road, which terminates at "L" Street. View to north. - South Omaha Union Stock Yards, 2900 "O" Plaza, Omaha, Douglas County, NE

  14. 2. FIRST FLOOR, FIRE ENGINE GARAGE, VIEW SHOWING STAIRWAY AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. FIRST FLOOR, FIRE ENGINE GARAGE, VIEW SHOWING STAIRWAY AND FIRE POLE (NOTE THE STAMPWORK ON WALL AND CEILING) - Hoboken Fire Engine Company Number Two, 1313 Washington Street, Hoboken, Hudson County, NJ

  15. 1. SHOWING RELATION OF FIRE CONTROL BUILDING, WATER TANK, AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. SHOWING RELATION OF FIRE CONTROL BUILDING, WATER TANK, AND TOWER, LOOKING SOUTH - Boswell Bay White Alice Site, Fire Control Building, Chugach National Forest, Cordova, Valdez-Cordova Census Area, AK

  16. 3. Oblique view of 215 Division Street, looking southeast, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Oblique view of 215 Division Street, looking southeast, showing rear (west) facade and north side, Fairbanks Company appears at left and 215 Division Street is visible at right - 215 Division Street (House), Rome, Floyd County, GA

  17. 1. Oblique view of 215 Division Street, looking southwest, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Oblique view of 215 Division Street, looking southwest, showing front (east) facade and north side, 213 Division Street is visible at left and 217 Division Street appears at right - 215 Division Street (House), Rome, Floyd County, GA

  18. 2. Oblique view of 215 Division Street, looking northeast, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Oblique view of 215 Division Street, looking northeast, showing rear (west) facade and south side, 217 Division Street is visible at left and Fairbanks Company appears at right - 215 Division Street (House), Rome, Floyd County, GA

  19. 3. Oblique view of 213 Division Street, looking northeast, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Oblique view of 213 Division Street, looking northeast, showing rear (west) facade and south side, 215 Division Street is visible at left and Fairbanks Company appears at right - 213 Division Street (House), Rome, Floyd County, GA

  20. 13. VIEW OF CENTER OF NORTH SECTION, SECOND FLOOR, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW OF CENTER OF NORTH SECTION, SECOND FLOOR, SHOWING TYPICAL SPACE AND STRUCTURAL CONFIGURATION, LOOKING EAST - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  1. 18. DETAIL VIEW SHOWING SAWTOOTH MONITOR ROOF AND TYPICAL STRUCTURAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. DETAIL VIEW SHOWING SAWTOOTH MONITOR ROOF AND TYPICAL STRUCTURAL CONFIGURATION OF TOP STORY, NORTH SECTION, THIRD FLOOR, LOOKING WEST INTO SOUTH SECTION - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  2. 12. VIEW ALONG SOUTHWEST SIDE OF SECOND FLOOR, SHOWING TYPICAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. VIEW ALONG SOUTHWEST SIDE OF SECOND FLOOR, SHOWING TYPICAL STRUCTURAL CONFIGURATION AND CONTEMPORARY PARTITIONING FOR OFFICES, LOOKING SOUTH - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  3. 1. VIEW OF SOUTHWEST ELEVATION SHOWING LOADING DOCK AND CANTED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW OF SOUTHWEST ELEVATION SHOWING LOADING DOCK AND CANTED CORNER WITH OFFICE ENTRANCE, LOOKING NORTHEAST. EASTERN CANAL IS IN FOREGROUND - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  4. 9. VIEW OF BASEMENT, NORTH SECTION, SHOWING TYPICAL PIER, FOOTINGS, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. VIEW OF BASEMENT, NORTH SECTION, SHOWING TYPICAL PIER, FOOTINGS, AND STRUCTURAL MEMBERS FOR FIRST FLOOR ABOVE, LOOKING WEST - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  5. 22. SOUTHWEST CORNER OF BANKING ROOM, FROM EAST, SHOWING CLOCK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. SOUTHWEST CORNER OF BANKING ROOM, FROM EAST, SHOWING CLOCK ON SOUTH WALL AND TWO MEZZANINES BEYOND COLUMNS - Philadelphia Saving Fund Society, Twelfth & Market Streets, Philadelphia, Philadelphia County, PA

  6. 4. VIEW FROM SOUTHWEST SHOWING WEST SIDE OF PIER AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW FROM SOUTHWEST SHOWING WEST SIDE OF PIER AND PEDESTRIAN BRIDGE CONNECTING MARINE MAMMAL PAVILION WITH BALTIMORE AQUARIUM - Baltimore Inner Harbor, Pier 4, South side of Pratt Street between Frederick Street & Market Place, Baltimore, Independent City, MD

  7. 10. GROUND VIEW OF PIER, LOOKING SOUTH FROM BEACH; SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. GROUND VIEW OF PIER, LOOKING SOUTH FROM BEACH; SHOWING (LEFT-RIGHT) CAPTAIN'S GALLEY'S GALLEY TO END OF PIER - Huntington Beach Municipal Pier, Pacific Coast Highway at Main Street, Huntington Beach, Orange County, CA

  8. BEACH ROAD SHOWING THE LAWN WITH KIAWE TREES BETWEEN THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BEACH ROAD SHOWING THE LAWN WITH KIAWE TREES BETWEEN THE ROAD AND THE BEACH. BEACH ROAD IS 14' WIDE. VIEW FACING SOUTH. - Hickam Field, Fort Kamehameha Historic Housing, Along Worchester Avenue & Hope Street, Honolulu, Honolulu County, HI

  9. 160. VIEW FROM NEW YORK HARBOR SHOWING THE JERSEY WATER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    160. VIEW FROM NEW YORK HARBOR SHOWING THE JERSEY WATER FRONT. PLEASE NOTE THAT ARROWS WERE ON ORIGINAL PHOTOGRAPH AND WHAT THEY POINT OUT IS NOT CERTAIN. - Morris Canal, Phillipsburg, Warren County, NJ

  10. 36. LARGE MOLD MAKING MACHINE, GREY IRON UNIT #4 SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. LARGE MOLD MAKING MACHINE, GREY IRON UNIT #4 SHOWING PATTERNS THAT FLASKS FIT OVER PRIOR TO BEING FILLED WITH SAND AND COMPRESSED. - Stockham Pipe & Fittings Company, Grey Iron Foundry, 4000 Tenth Avenue North, Birmingham, Jefferson County, AL

  11. OVERALL VIEW OF SOUTHERN DUCTILE'S PATTERN REPAIR SHOP, SHOWING A ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OVERALL VIEW OF SOUTHERN DUCTILE'S PATTERN REPAIR SHOP, SHOWING A SPANISH-MADE FORADIA BORING MACHINE IN THE FOREGROUND. - Southern Ductile Casting Company, Mold Making, 2217 Carolina Avenue, Bessemer, Jefferson County, AL

  12. Center pivot, showing substantial beams that support the trusses. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Center pivot, showing substantial beams that support the trusses. Looking north from civilian land. - Naval Supply Annex Stockton, Daggett Road Bridge, Daggett Road traversing Burns Cut Off, Stockton, San Joaquin County, CA

  13. Photocopy of "sheet 3 of 8" showing roof framing plan, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of "sheet 3 of 8" showing roof framing plan, ceiling plan, ceiling hangers and wall ties, cross section of rafters, and front elevation. - Badger Mountain Lookout, .125 mile northwest of Badger Mountain summit, East Wenatchee, Douglas County, WA

  14. Photocopy of "sheet 2 of 8" showing front, side and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of "sheet 2 of 8" showing front, side and rear elevations and the framing for those elevations. - Badger Mountain Lookout, .125 mile northwest of Badger Mountain summit, East Wenatchee, Douglas County, WA

  15. Photocopy of "sheet 6 of 8" showing cross section of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of "sheet 6 of 8" showing cross section of house, front elevation, fire finder stand, hip roof cap, and shiplap roof sheathing. - Badger Mountain Lookout, .125 mile northwest of Badger Mountain summit, East Wenatchee, Douglas County, WA

  16. 10. VIEW OF THE BADGER STATE MINEYARD, LOOKING NORTHEAST, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. VIEW OF THE BADGER STATE MINEYARD, LOOKING NORTHEAST, SHOWING THE HOIST HOUSE ON THE LEFT, THE IDLER TOWER, AND A LEG OF THE HEADFRAME ON THE RIGHT - Butte Mineyards, Badger State Mine, Butte, Silver Bow County, MT

  17. Detail of tower support construction shows two legs, cross beams ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of tower support construction shows two legs, cross beams end concrete point load footing on west side. Camera is pointed NW. - Badger Mountain Lookout, .125 mile northwest of Badger Mountain summit, East Wenatchee, Douglas County, WA

  18. Photocopy of "sheet 4 of 8" showing window details, door ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of "sheet 4 of 8" showing window details, door sill detail, vertical wall sections, and cross sections thru front, side and rear elevations. - Badger Mountain Lookout, .125 mile northwest of Badger Mountain summit, East Wenatchee, Douglas County, WA

  19. 12. VIEW, LOOKING NORTH, SHOWING CABLEWAY AND TRAM ABOVE AN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. VIEW, LOOKING NORTH, SHOWING CABLEWAY AND TRAM ABOVE AN ARCH UNDER CONSTRUCTION, WITH RAILROAD BRIDGE IN BACKGROUND - Cotter Bridge, Spanning White River at U.S. Highway 62, Cotter, Baxter County, AR

  20. 17. VIEW OF NORTH BRIDGE PORTAL, SHOWING ORNAMENTAL UPPER PORTAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. VIEW OF NORTH BRIDGE PORTAL, SHOWING ORNAMENTAL UPPER PORTAL STRUT AND GEOMETRIC DECORATED GUSSET. FACING NORTHEAST. - Coverts Crossing Bridge, Spanning Mahoning River along Township Route 372 (Covert Road), New Castle, Lawrence County, PA

  1. 2. FULL VIEW OF BRIDGE, SHOWING INCLINED END POST (HATTER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. FULL VIEW OF BRIDGE, SHOWING INCLINED END POST (HATTER POST), PORTAL BRACING, AND PORTAL STRUT AT EAST PORTAL, LOOKING WEST - Chester Bridge, Spanning Rock River on Old Marsh Road, East Waupun, Dodge County, WI

  2. 15. VIEW OF SOUTH BRIDGE PORTAL, SHOWING ORNAMENTAL UPPER PORTAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. VIEW OF SOUTH BRIDGE PORTAL, SHOWING ORNAMENTAL UPPER PORTAL STRUT AND GUSSETS (FLORAL MOTIF). FACING SOUTHWEST. - Coverts Crossing Bridge, Spanning Mahoning River along Township Route 372 (Covert Road), New Castle, Lawrence County, PA

  3. Detail of framing and planking near midship section showing old ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of framing and planking near midship section showing old frames and planks with bulwark timbers above. - Schooner ERNESTINA, New Bedford Whaling National Historical Park State Pier, New Bedford, Bristol County, MA

  4. 3. VIEW OF UPSTREAM FACE OF DAM, SHOWING OUTLET GATE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW OF UPSTREAM FACE OF DAM, SHOWING OUTLET GATE, LOOKING NORTHEAST - High Mountain Dams in Upalco Unit, Island Lake Dam, Ashley National Forest, 4.8 miles North of Miners Gulch Campground, Mountain Home, Duchesne County, UT

  5. 3. VIEW TO WEST SHOWING NORTHEAST SIDE OF BUILDING. NOTE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW TO WEST SHOWING NORTHEAST SIDE OF BUILDING. NOTE STIFFENING TRUSS OVER DOORWAY TO SUPPORT THE WALL. - Rosie the Riveter National Historical Park, Auxiliary Plate Shop, 912 Harbour Way, Richmond, Contra Costa County, CA

  6. 13. VIEW TO SOUTHEAST OF WAITING AREA SHOWING STAIRS TO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW TO SOUTHEAST OF WAITING AREA SHOWING STAIRS TO FIRST FLOOR (LEFT) AND HALL TO MANAGERS OFFICES (RIGHT). - Rosie the Riveter National Historical Park, Ford Assembly Plant, 1400 Harbour Way South, Richmond, Contra Costa County, CA

  7. VIEW EAST SHOWING THE WEST SIDE OF THE FORWARD (BOW) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW EAST SHOWING THE WEST SIDE OF THE FORWARD (BOW) SECTION - Rosie the Riveter National Historical Park, Rosie the Riveter Memorial, Off Regatta at Melville Square in Marina Park, Richmond, Contra Costa County, CA

  8. 5. GENERAL WAREHOUSE, PERSPECTIVE VIEW TO NORTHWEST SHOWING FRONT ELEVATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. GENERAL WAREHOUSE, PERSPECTIVE VIEW TO NORTHWEST SHOWING FRONT ELEVATION (LEFT) AND NORTH SIDE (RIGHT). - Rosie the Riveter National Historical Park, General Warehouse, 1320 Canal Boulevard, Richmond, Contra Costa County, CA

  9. 7. GENERAL WAREHOUSE, PERSPECTIVE VIEW TO NORTHWEST SHOWING FRONT ELEVATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. GENERAL WAREHOUSE, PERSPECTIVE VIEW TO NORTHWEST SHOWING FRONT ELEVATION (LEFT) AND NORTH SIDE (RIGHT). - Rosie the Riveter National Historical Park, General Warehouse, 1320 Canal Boulevard, Richmond, Contra Costa County, CA

  10. 3. VIEW TO WEST SHOWING EAST END OF OIL HOUSE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW TO WEST SHOWING EAST END OF OIL HOUSE (LEFT) AND EAST SIDE OF CRATING SHED AND ASSEMBLY BUILDING (RIGHT BACKGROUND). - Rosie the Riveter National Historical Park, Ford Assembly Plant, 1400 Harbour Way South, Richmond, Contra Costa County, CA

  11. 6. GENERAL WAREHOUSE, VIEW TO EAST SHOWING DETAIL OF ELEVATOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. GENERAL WAREHOUSE, VIEW TO EAST SHOWING DETAIL OF ELEVATOR DOOR (CENTER), FLANKING PEDESTRIAN ENTRIES, AND LOADING DOCK. - Rosie the Riveter National Historical Park, General Warehouse, 1320 Canal Boulevard, Richmond, Contra Costa County, CA

  12. 7. VIEW TO EAST SHOWING ELEVATION OF THE OFFICE PORTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. VIEW TO EAST SHOWING ELEVATION OF THE OFFICE PORTION OF THE STRUCTURE AT NORTHWEST CORNER. - Rosie the Riveter National Historical Park, Ford Assembly Plant, 1400 Harbour Way South, Richmond, Contra Costa County, CA

  13. CAFETERIA AT SHIPYARD NO. 3, PERSPECTIVE VIEW TO NORTHWEST SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CAFETERIA AT SHIPYARD NO. 3, PERSPECTIVE VIEW TO NORTHWEST SHOWING SOUTHEAST END AND FRONT SIDE (SHADED BY TREES) - Rosie the Riveter National Historical Park, Cafeteria, 1301 Canal Boulevard, Richmond, Contra Costa County, CA

  14. VIEW SOUTH SHOWING THE STRUCTURE REPRESENTING THE FORWARD (BOW) SECTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW SOUTH SHOWING THE STRUCTURE REPRESENTING THE FORWARD (BOW) SECTION OF A LIBERTY SHIP - Rosie the Riveter National Historical Park, Rosie the Riveter Memorial, Off Regatta at Melville Square in Marina Park, Richmond, Contra Costa County, CA

  15. 1. PERSPECTIVE VIEW TO WEST SHOWING SOUTHEAST END. NOTE THAT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. PERSPECTIVE VIEW TO WEST SHOWING SOUTHEAST END. NOTE THAT EXTERIOR CRANEWAY TRESTLES HAVE BEEN REDUCED IN LENGTH. - Rosie the Riveter National Historical Park, Auxiliary Plate Shop, 912 Harbour Way, Richmond, Contra Costa County, CA

  16. 4. GENERAL WAREHOUSE, PERSPECTIVE VIEW TO NORTHWEST SHOWING SOUTH SIDE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. GENERAL WAREHOUSE, PERSPECTIVE VIEW TO NORTHWEST SHOWING SOUTH SIDE (LEFT) AND FRONT ELEVATION (RIGHT). - Rosie the Riveter National Historical Park, General Warehouse, 1320 Canal Boulevard, Richmond, Contra Costa County, CA

  17. 4. VIEW TO EAST SHOWING WEST END OF CRANEWAY (CENTER), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. VIEW TO EAST SHOWING WEST END OF CRANEWAY (CENTER), WEST SIDE OF ASSEMBLY BUILDING (LEFT), AND WHARF (RIGHT). - Rosie the Riveter National Historical Park, Ford Assembly Plant, 1400 Harbour Way South, Richmond, Contra Costa County, CA

  18. 2. VIEW TO SOUTHWEST SHOWING OIL HOUSE (LEFT), BOILER ROOM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW TO SOUTHWEST SHOWING OIL HOUSE (LEFT), BOILER ROOM AND STACK (CENTER), ROOF OF CRANEWAY (BACKGROUND), AND CRATING SHED (RIGHT). - Rosie the Riveter National Historical Park, Ford Assembly Plant, 1400 Harbour Way South, Richmond, Contra Costa County, CA

  19. 15. Elevator no. 2: Scale floor above track shed, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Elevator no. 2: Scale floor above track shed, showing interlocking levers and beams, facing southeast - Washburn Crosby Company Elevators No. 2 & 3, 900 & 1000 Second Avenue, South, Minneapolis, Hennepin County, MN

  20. INTERIOR VIEW OF WEST BANK RAILROAD CONTROL TOWER SHOWING INTERLOCKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR VIEW OF WEST BANK RAILROAD CONTROL TOWER SHOWING INTERLOCKING SWITCHING LEVER CONTROL PANEL. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  1. 9. Detail of end post connection on south web, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Detail of end post connection on south web, showing vertical, end posts, pin and pin plate. View to south. - Slough Creek Bridge, Spanning Slough Creek at CART Road 330, Arrow Rock, Saline County, MO

  2. 8. DETAIL VIEW OF PIN CONNECTION, NORTH WEB, SHOWING FLOOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. DETAIL VIEW OF PIN CONNECTION, NORTH WEB, SHOWING FLOOR BEAM, STRINGERS, LATTICE BRACING, EYEBARS, AND PIN, LOOKING SOUTH - Four Mile Bridge, Spanning Elk River on County Road 42, Steamboat Springs, Routt County, CO

  3. Detail of northeast corner showing metal plates and poles of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of northeast corner showing metal plates and poles of unknown purpose. - Fitzsimons General Hospital, Nurses' Quarters, Southwest Corner of West Harlow Avenue, & South Eighth Street, Aurora, Adams County, CO

  4. Building D interior, looking south showing same stone wall as ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Building D interior, looking south showing same stone wall as it attaches to the east wall of Building D - Hinckley Knitting Mills, Building D, 21-35 East Wister Street, Philadelphia, Philadelphia County, PA

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    ... 164100.html Intensive Treatment Shows Potential Against Type 2 Diabetes Up to 40 percent experienced temporary remission, ... 15, 2017 (HealthDay News) -- Instead of managing type 2 diabetes as a chronic condition, what if people ...

  6. Antenna cab interior showing equipment rack and fiberglass antenna panels, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Antenna cab interior showing equipment rack and fiberglass antenna panels, looking southeast. - Western Union Telegraph Company, Jennerstown Relay, Laurel Summit Road off U.S. 30, Laughlintown, Westmoreland County, PA

  7. Antenna cab interior showing waveguide from external parabolic antenna (later ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Antenna cab interior showing waveguide from external parabolic antenna (later addition), looking north. - Western Union Telegraph Company, Jennerstown Relay, Laurel Summit Road off U.S. 30, Laughlintown, Westmoreland County, PA

  8. Antenna cab interior showing equipment rack and fiberglass antenna panels, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Antenna cab interior showing equipment rack and fiberglass antenna panels, looking west. - Western Union Telegraph Company, Jennerstown Relay, Laurel Summit Road off U.S. 30, Laughlintown, Westmoreland County, PA

  9. 19. WINDOW DETAIL, NORTH WALL OF GARAGE ADDITION. VIEW SHOWS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. WINDOW DETAIL, NORTH WALL OF GARAGE ADDITION. VIEW SHOWS CONCRETE BLOCK CONSTRUCTION OF ADDITION. - Chollas Heights Naval Radio Transmitting Facility, Transmitter Building, 6410 Zero Road, San Diego, San Diego County, CA

  10. 3. VIEW SHOWING RELATIONSHIP OF EAST SIDES OF BUILDINGS 15 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW SHOWING RELATIONSHIP OF EAST SIDES OF BUILDINGS 15 ON LEFT, BUILDING 40 GARAGE IN CENTER, AND BUILDING 4 IN DISTANCE. - Chollas Heights Naval Radio Transmitting Facility, 6410 Zero Road, San Diego, San Diego County, CA

  11. 1. WEST SIDE, NORTH END OF BUILDING 1. SHOWS ADDITIONAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. WEST SIDE, NORTH END OF BUILDING 1. SHOWS ADDITIONAL ANGLES IN BUILDING AT SOUTHERN END. - Chollas Heights Naval Radio Transmitting Facility, Transmitter Building, 6410 Zero Road, San Diego, San Diego County, CA

  12. 9. Detail Showing top of Samson Post and Walking Beam ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Detail Showing top of Samson Post and Walking Beam Pivot, Looking East - David Renfrew Oil Rig, East side of Connoquenessing Creek, 0.4 mile North of confluence with Thorn Creek, Renfrew, Butler County, PA

  13. Rod shop, roof and truss detail showing older pink roof ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Rod shop, roof and truss detail showing older pink roof truss, newer pratt truss, and longitudinal, truss for overhead traveling crane - Chicago, Burlington & Quincy Railroad, Roundhouse & Shops, Broadway & Spring Streets, Aurora, Kane County, IL

  14. INTERIOR OF BUILDING, SHOWING PIPELINES. view TO NORTH Plattsburgh ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR OF BUILDING, SHOWING PIPELINES. view TO NORTH - Plattsburgh Air Force Base, Industrial Wastewater Treatment & Disposal Facility, Off LeMay Road, outside SAC Alert Area, Plattsburgh, Clinton County, NY

  15. 44. DETAIL OF WALL SHOWING 1914 CALENDAR (DEPICTING PANAMA CANAL), ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    44. DETAIL OF WALL SHOWING 1914 CALENDAR (DEPICTING PANAMA CANAL), PATTERN FOR NARROW GAUGE RR WHEEL, AND AD-LOOKING SOUTHEAST. - W. A. Young & Sons Foundry & Machine Shop, On Water Street along Monongahela River, Rices Landing, Greene County, PA

  16. FIXED END OF MIDDLE SPAN. WESTERN SIDE SHOWING WELDING OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    FIXED END OF MIDDLE SPAN. WESTERN SIDE SHOWING WELDING OF TOP PLATE ADDED TO STRENGTHEN THE BRIDGE. - Spile Bridge Road Bridge, Spanning Black Lake Outlie at Spile Bridge Road, Oswegatchie, St. Lawrence County, NY

  17. CENTER AISLE, WEST STORAGE AREA, FROM EAST, SHOWING ADDED WIRE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CENTER AISLE, WEST STORAGE AREA, FROM EAST, SHOWING ADDED WIRE MESH, GYPSUM BOARD PARTITIONS, AND EXPOSED CEILING FRAMING - Fort Huachuca, Cavalry Stable, Clarkson Road, Sierra Vista, Cochise County, AZ

  18. 15. VIEW SHOWING FRONT OF MESS HALL, LOOKING NORTH. STORE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. VIEW SHOWING FRONT OF MESS HALL, LOOKING NORTH. STORE WORK WAS SUBSEQUENTLY ADDED Marilyn Ziemer, photographer, April 1988 - Los Pinetos Nike Missile Site, Santa Clara Road, Los Angeles National Forest, Sylmar, Los Angeles County, CA

  19. Close view of the Constitution Avenue elevation to show arched ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Close view of the Constitution Avenue elevation to show arched gateways and pedimented pavilion ("Commerce and Communication" sculpted pediment) - Interstate Commerce Commission, Constitution Avenue between Twelfth and Fourteenth streets, Washington, District of Columbia, DC

  20. Context view shows approach of access road to summit, communication ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Context view shows approach of access road to summit, communication towers and NW corner of lookout tower at center right. Camera is pointed SE. - Chelan Butte Lookout, Summit of Chelan Butte, Chelan, Chelan County, WA

  1. 20. VIEW OF EAST END OF SPERRY OCEAN DOCK, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. VIEW OF EAST END OF SPERRY OCEAN DOCK, SHOWING SOUTH AND EAST ELEVATION, INCLUDING PORTION OF THE SPERRY OCEAN DOCK DECK, LOOKING NORTH - Puget Sound Flouring Mills, 611 Schuster Parkway, Tacoma, Pierce County, WA

  2. 1. GENERAL VIEW SHOWING NORTHEAST END (FRONT) OF TRANSIT SHED, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. GENERAL VIEW SHOWING NORTHEAST END (FRONT) OF TRANSIT SHED, IN CONTEXT WITH LOADING YARD AND DERRICK, LOOKING WEST - Oakland Army Base, Transit Shed, East of Dunkirk Street & South of Burma Road, Oakland, Alameda County, CA

  3. 20. DETAIL VIEW OF SUBMERSIBLE GATE, SHOWING GATE ARMS, GATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. DETAIL VIEW OF SUBMERSIBLE GATE, SHOWING GATE ARMS, GATE PIERS, TRUNNION PIN AND GATE GAUGE, LOOKING NORTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 8, On Mississippi River near Houston County, MN, Genoa, Vernon County, WI

  4. 19. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE ARM, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE ARM, PIER, TRUNNION PIN AND GATE GAUGE, LOOKING NORTH - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 8, On Mississippi River near Houston County, MN, Genoa, Vernon County, WI

  5. 21. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE, GATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE, GATE ARM, TRUNNION PIN, PIER AND GATE GAUGE, LOOKING EAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 10, Guttenberg, Clayton County, IA

  6. 23. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING TRUNNION PIN, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING TRUNNION PIN, GATE ARM AND GATE GAUGE, LOOKING SOUTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 10, Guttenberg, Clayton County, IA

  7. 16. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE, GATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE, GATE ARM, TRUNNION PIN AND PIER, LOOKING NORTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 8, On Mississippi River near Houston County, MN, Genoa, Vernon County, WI

  8. 17. DETAIL VIEW OF TAINTER GATE, SHOWING SUBMERSIBLE (LEFT) AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. DETAIL VIEW OF TAINTER GATE, SHOWING SUBMERSIBLE (LEFT) AND NONSUBMERSIBLE (RIGHT) GATES, PIERS AND DAM BRIDGE, WITH ROLLER GATE HEADHOUSE IN BACKGROUND, LOOKING NORTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 10, Guttenberg, Clayton County, IA

  9. 21. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE ARM, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATE ARM, GATE PIER, TRUNNION PIN AND GATE GAUGE, LOOKING SOUTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 9, Lynxville, Crawford County, WI

  10. 11. DETAIL VIEW OF NONSUBMERSIBLE TAINTER GATE, SHOWING CABLE ASSEMBLY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. DETAIL VIEW OF NON-SUBMERSIBLE TAINTER GATE, SHOWING CABLE ASSEMBLY ATTACHMENT, LOOKING EAST (DOWNSTREAM) - Upper Mississippi River 9-Foot Channel Project, Lock & Dam 26R, Alton, Madison County, IL

  11. 10. DETAIL OF NONOVERFLOW SECTION OF DAM SHOWING PENSTOCK OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. DETAIL OF NON-OVERFLOW SECTION OF DAM SHOWING PENSTOCK OF SUBMERSIBLE TURBINE-GENERATOR - Middle Creek Hydroelectric Dam, On Middle Creek, West of U.S. Route 15, 3 miles South of Selinsgrove, Selinsgrove, Snyder County, PA

  12. 21. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING TRUNNION PIN, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING TRUNNION PIN, LOOKING NORTH - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 8, On Mississippi River near Houston County, MN, Genoa, Vernon County, WI

  13. 18. DETAIL VIEW OF NONSUBMERSIBLE TAINTER GATE, SHOWING GATES, GATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. DETAIL VIEW OF NON-SUBMERSIBLE TAINTER GATE, SHOWING GATES, GATE ARMS, PIERS AND DAM BRIDGE, LOOKING SOUTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 9, Lynxville, Crawford County, WI

  14. 17. DETAIL VIEW OF NONSUBMERSIBLE TAINTER GATE, SHOWING GATES, GATE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. DETAIL VIEW OF NON-SUBMERSIBLE TAINTER GATE, SHOWING GATES, GATE ARMS, PIERS AND DAM BRIDGE, WITH ROLLER GATE HEADHOUSE IN BACKGROUND, LOOKING SOUTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 9, Lynxville, Crawford County, WI

  15. 15. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATES AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. DETAIL VIEW OF SUBMERSIBLE TAINTER GATE, SHOWING GATES AND GATE ARMS, PIERS AND DAM BRIDGE, LOOKING NORTHEAST - Upper Mississippi River 9-Foot Channel, Lock & Dam No. 8, On Mississippi River near Houston County, MN, Genoa, Vernon County, WI

  16. CLOSE VIEW ALONG WATERFRONT TO SHOW BULKHEAD Southwest Washington, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    CLOSE VIEW ALONG WATERFRONT TO SHOW BULKHEAD - Southwest Washington, Urban Renewal Area, Bounded by Independence Avenue, Washington Avenue, South Capitol Street, Canal Street, P Street, Maine Avenue & Washington Channel, Fourteenth Street, D Street, & Twelfth Street, Washington, District of Columbia, DC

  17. 1. OVERALL VIEW OF BRIDGE AND LINCOLN HIGHWAY, SHOWING NORTH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. OVERALL VIEW OF BRIDGE AND LINCOLN HIGHWAY, SHOWING NORTH APPROACH TO BRIDGE. VIEW TO SOUTH. - Rock Valley Bridge, Spanning North Timber Creek at Old U.S. Highway 30, Marshalltown, Marshall County, IA

  18. 10. Photocopy of circa 1948 photograph showing a view of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Photocopy of circa 1948 photograph showing a view of the Waiting Room Access of Train Shed, courtesy of Reading Company Archives - Philadelphia & Reading Railroad, Terminal Station, 1115-1141 Market Street, Philadelphia, Philadelphia County, PA

  19. 11. Photocopy of circa 1948 photograph showing detail view of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photocopy of circa 1948 photograph showing detail view of Waiting Room ceiling, courtesy of Reading Company Archives - Philadelphia & Reading Railroad, Terminal Station, 1115-1141 Market Street, Philadelphia, Philadelphia County, PA

  20. 1. VIEW OF HOSPITAL COMPLEX FROM MOVIE THEATER, SHOWING SOUTHEAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW OF HOSPITAL COMPLEX FROM MOVIE THEATER, SHOWING SOUTHEAST SECTION OF COMPLEX - Fort Randall, Neuro-Psychiatric Ward, Northeast of intersection of California Boulevard & Nurse Drive, Cold Bay, Aleutian Islands, AK

  1. View of first level from north showing interstitial structural columns ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of first level from north showing interstitial structural columns for the Shuttle assemble configuration. - Marshall Space Flight Center, Saturn V Dynamic Test Facility, East Test Area, Huntsville, Madison County, AL

  2. 8. Photocopy of Station Grounding drawing showing floor plans (from ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Photocopy of Station Grounding drawing showing floor plans (from the BPA Engineering Vault, Drawing 005319, 3 May 1939) - Bonneville Power Administration South Bank Substation, I-84, South of Bonneville Dam Powerhouse, Bonneville, Multnomah County, OR

  3. 12. Interior view of cement and aggregate batch plant showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Interior view of cement and aggregate batch plant showing storage bins. Photographer unknown, c. 1926. Source: Ralph Pleasant. - Waddell Dam, On Agua Fria River, 35 miles northwest of Phoenix, Phoenix, Maricopa County, AZ

  4. 44. INTERIOR DETAIL OF OLD RESERVOIR AT KALAWAO SHOWING CEMENTLINED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    44. INTERIOR DETAIL OF OLD RESERVOIR AT KALAWAO SHOWING CEMENT-LINED RUBBLE CONSTRUCTION. - Kalaupapa Water Supply System, Waikolu Valley to Kalaupapa Settlement, Island of Molokai, Kalaupapa, Kalawao County, HI

  5. 4. Cement and Plaster Warehouse, southeast corner, showing alterations; pent ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Cement and Plaster Warehouse, southeast corner, showing alterations; pent roof, window and door openings, siding, brick foundation sheathing. - Curtis Wharf, Cement & Plaster Warehouse, O & Second Streets, Anacortes, Skagit County, WA

  6. View east, stone sluice, beginning of lower standing section, showing ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View east, stone sluice, beginning of lower standing section, showing third drop, stone pier in center, cement piers to right - Glens Falls Feeder, Sluice, Along south side of Glens Falls Feeder between locks 10 & 20, Hudson Falls, Washington County, NY

  7. 4. INTERIOR OF ABANDONED SANTA ANA CANAL TUNNEL, SHOWING CEMENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. INTERIOR OF ABANDONED SANTA ANA CANAL TUNNEL, SHOWING CEMENT TROUGH FLOOR AND UNFINISHED GRANITE ROOF. VIEW TO SOUTHWEST. - Santa Ana River Hydroelectric System, Abandoned Tunnel, Redlands, San Bernardino County, CA

  8. View northwest, discharge basin, floor, showing cement cross beams built ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View northwest, discharge basin, floor, showing cement cross beams built on stone bases - Glens Falls Feeder, Sluice, Along south side of Glens Falls Feeder between locks 10 & 20, Hudson Falls, Washington County, NY

  9. 19. INTERIOR VIEW INSIDE BUNKER SHOWING NITROGEN TANKS, 'MOBILE AIR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. INTERIOR VIEW INSIDE BUNKER SHOWING NITROGEN TANKS, 'MOBILE AIR MONITOR' EQUIPMENT, MAN. INEL PHOTO NUMBER 65-6183, TAKEN NOVEMBER 10, 1965. - Idaho National Engineering Laboratory, Advanced Reentry Vehicle Fusing System, Scoville, Butte County, ID

  10. 18. June 1974. BASEMENT, VIEW LOOKING NORTHWEST, SHOWING BELTING AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. June 1974. BASEMENT, VIEW LOOKING NORTHWEST, SHOWING BELTING AND ENGAGING MECHANISM FOR DRIVING THE TABLE SAW. - Gruber Wagon Works, Pennsylvania Route 183 & State Hill Road at Red Bridge Park, Bernville, Berks County, PA

  11. 1. Photocopy of photograph. VIEW NORTH SHOWING SOUTH ELEVATION (Original ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of photograph. VIEW NORTH SHOWING SOUTH ELEVATION (Original print in the possession of Engineering Photograph Collection, Municipal Archives, city of Rochester, New York, c. 1890) - Driving Park Avenue Bridge, Spanning Genesee River Gorge, Rochester, Monroe County, NY

  12. 1. VIEW SOUTHEAST SHOWING NORTHWEST (FRONT) ELEVATIONS OF 220 AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW SOUTHEAST SHOWING NORTHWEST (FRONT) ELEVATIONS OF 220 AND 218 KING STREET (HABS NO. DE-129-F) - King Street, 200 Block, 220 King Street (Commercial Building), Wilmington, New Castle County, DE

  13. 1. VIEW SOUTHEAST SHOWING NORTHWEST (FRONT) ELEVATIONS OF 214 AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW SOUTHEAST SHOWING NORTHWEST (FRONT) ELEVATIONS OF 214 AND 212 KING STREET (HABS NO. DE-129-I) - King Street, 200 Block, 214 King Street (Commercial Building), Wilmington, New Castle County, DE

  14. 38. INTERIOR VIEW OF FOUNDRY SHOWING HEAVY TIMBER ROOF TRUSSES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. INTERIOR VIEW OF FOUNDRY SHOWING HEAVY TIMBER ROOF TRUSSES AND TRAVELING CRANE. - Baltimore & Ohio Railroad, Mount Clare Shops, South side of Pratt Street between Carey & Poppleton Streets, Baltimore, Independent City, MD

  15. 5. MAIN BAY SHOWING ROOF CONSTRUCTION, ROOF TRUSS, CLERESTORY MONITOR, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. MAIN BAY SHOWING ROOF CONSTRUCTION, ROOF TRUSS, CLERESTORY MONITOR, AND GIRDER FOR ELECTRIC OVERHEAD TRAVEL CRANE (BOTTOM) - Oldman Boiler Works, Boilershop, 32 Illinois Street, Buffalo, Erie County, NY

  16. Interior view to show detail of the portal windows and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view to show detail of the portal windows and muntins creating a star-like pattern - National Park Seminary, Chateau Causeways, Between Linden Lane & Beach Drive, Silver Spring, Montgomery County, MD

  17. "BURBANK FARM" SHOWING BANK CUT CREATED WHEN BODEGA AVENUE ROADBED ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    "BURBANK FARM" SHOWING BANK CUT CREATED WHEN BODEGA AVENUE ROADBED WAS LOWERED. WPA STONE RETAINING WALL AT BASE OF CUT. LOOKING SOUTHWEST. - Gold Ridge Farm, 7777 Bodega Avenue, Sebastopol, Sonoma County, CA

  18. Detail of back of lion showing need for restoration, northeast ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of back of lion showing need for restoration, northeast corner of bridge. - Connecticut Avenue Bridge, Spans Rock Creek & Potomac Parkway at Connecticut Avenue, Washington, District of Columbia, DC

  19. INTERIOR DETAIL OF, CEILINGS OF EAST BEDROOM, NORTH WING, SHOWING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    INTERIOR DETAIL OF, CEILINGS OF EAST BEDROOM, NORTH WING, SHOWING PART OF MOUNTAIN LION MURAL; CAMERA FACING NORTHEAST - Harry Carey Ranch, Ranch House, 28515 San Francisquito Canyon Road, Saugus, Los Angeles County, CA

  20. Detail view in caryatid breezeway to show entrance steps; lion ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view in caryatid breezeway to show entrance steps; lion statues once flanked these steps - National Park Seminary, Aloha House, North of Linden Lane near corner of Beech Drive, Silver Spring, Montgomery County, MD