Overhead View of Area Surrounding Pathfinder

NASA Technical Reports Server (NTRS)

1997-01-01

Overhead view of the area surrounding the Pathfinder lander illustrating the Sojourner traverse. Red rectangles are rover positions at the end of sols 1-30. Locations of soil mechanics experiments, wheel abrasion experiments, and APXS measurements are shown. The A numbers refer to APXS measurements as discussed in the paper by Rieder et al. (p. 1770, Science Magazine, see image note). Coordinates are given in the LL frame.

The photorealistic, interactive, three-dimensional virtual reality (VR) terrain models were created from IMP images using a software package developed for Pathfinder by C. Stoker et al. as a participating science project. By matching features in the left and right camera, an automated machine vision algorithm produced dense range maps of the nearfield, which were projected into a three-dimensional model as a connected polygonal mesh. Distance and angle measurements can be made on features viewed in the model using a mouse-driven three-dimensional cursor and a point-and-click interface. The VR model also incorporates graphical representations of the lander and rover and the sequence and spatial locations at which rover data were taken. As the rover moved, graphical models of the rover were added for each position that could be uniquely determined using stereo images of the rover taken by the IMP. Images taken by the rover were projected into the model as two-dimensional 'billboards' to show the proper perspective of these images.

NOTE: original caption as published in Science Magazine

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Terrain at Landing Site

NASA Image and Video Library

1997-07-05

Portions of Mars Pathfinder's deflated airbags (seen in the foreground), a large rock in mid-field, and a hill in the background were taken by the Imager for Mars Pathfinder (IMP) aboard Mars Pathfinder during the spacecraft's first day on the Red Planet. Pathfinder successfully landed on Mars at 10:07 a.m. PDT earlier today. The IMP is a stereo imaging system with color capability provided by 24 selectable filters -- twelve filters per "eye." It stands 1.8 meters above the Martian surface, and has a resolution of two millimeters at a range of two meters. http://photojournal.jpl.nasa.gov/catalog/PIA00615

Big Crater as Viewed by Pathfinder Lander

NASA Technical Reports Server (NTRS)

1997-01-01

The 'Big Crater' is actually a relatively small Martian crater to the southeast of the Mars Pathfinder landing site. It is 1500 meters (4900 feet) in diameter, or about the same size as Meteor Crater in Arizona. Superimposed on the rim of Big Crater (the central part of the rim as seen here) is a smaller crater nicknamed 'Rimshot Crater.' The distance to this smaller crater, and the nearest portion of the rim of Big Crater, is 2200 meters (7200 feet). To the right of Big Crater, south from the spacecraft, almost lost in the atmospheric dust 'haze,' is the large streamlined mountain nicknamed 'Far Knob.' This mountain is over 450 meters (1480 feet) tall, and is over 30 kilometers (19 miles) from the spacecraft. Another, smaller and closer knob, nicknamed 'Southeast Knob' can be seen as a triangular peak to the left of the flanks of the Big Crater rim. This knob is 21 kilometers (13 miles) southeast from the spacecraft.

The larger features visible in this scene - Big Crater, Far Knob, and Southeast Knob - were discovered on the first panoramas taken by the IMP camera on the 4th of July, 1997, and subsequently identified in Viking Orbiter images taken over 20 years ago. The scene includes rocky ridges and swales or 'hummocks' of flood debris that range from a few tens of meters away from the lander to the distance of South Twin Peak. The largest rock in the nearfield, just left of center in the foreground, nicknamed 'Otter', is about 1.5 meters (4.9 feet) long and 10 meters (33 feet) from the spacecraft.

This view of Big Crater was produced by combining 6 individual 'Superpan' scenes from the left and right eyes of the IMP camera. Each frame consists of 8 individual frames (left eye) and 7 frames (right eye) taken with different color filters that were enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The IMP was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Sojourner, Wedge, & Shark

NASA Technical Reports Server (NTRS)

1997-01-01

This Imager for Mars Pathfinder (IMP) image taken near the end of daytime operations on Sol 50 shows the Sojourner rover between the rocks 'Wedge' (foreground) and 'Shark' (behind rover). The rover successfully deployed its Alpha Proton X-Ray Spectrometer on Shark on Sol 52.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Sojourner's APXS at Shark

NASA Technical Reports Server (NTRS)

1997-01-01

The Sojourner rover is seen next to the rock 'Shark', in this image taken by the Imager for Mars Pathfinder (IMP) near the end of daytime operations on Sol 52. The rover's Alpha Proton X-Ray Spectrometer is deployed against the rock. The rock 'Wedge' is in the foreground.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Mineralogic and compositional properties of Martian soil and dust: results from Mars Pathfinder

USGS Publications Warehouse

Bell, J.F.; McSween, H.Y.; Crisp, J.A.; Morris, R.V.; Murchie, S.L.; Bridges, N.T.; Johnson, J. R.; Britt, D.T.; Golombek, M.P.; Moore, H.J.; Ghosh, A.; Bishop, J.L.; Anderson, R.C.; Brückner, J.; Economou, T.; Greenwood, J.P.; Gunnlaugsson, H.P.; Hargraves, R.M.; Hviid, S.; Knudsen, J.M.; Madsen, M.B.; Reid, R.; Rieder, R.; Soderblom, L.

2000-01-01

Mars Pathfinder obtained multispectral, elemental, magnetic, and physical measurements of soil and dust at the Sagan Memorial Station during the course of its 83 sol mission. We describe initial results from these measurements, concentrating on multispectral and elemental data, and use these data, along with previous Viking, SNC meteorite, and telescopic results, to help constrain the origin and evolution of Martian soil and dust. We find that soils and dust can be divided into at least eight distinct spectral units, based on parameterization of Imager for Mars Pathfinder (IMP) 400 to 1000 nm multispectral images. The most distinctive spectral parameters for soils and dust are the reflectivity in the red, the red/blue reflectivity ratio, the near-IR spectral slope, and the strength of the 800 to 1000 nm absorption feature. Most of the Pathfinder spectra are consistent with the presence of poorly crystalline or nanophase ferric oxide(s), sometimes mixed with small but varying degrees of well-crystalline ferric and ferrous phases. Darker soil units appear to be coarser-grained, compacted, and/or mixed with a larger amount of dark ferrous materials relative to bright soils. Nanophase goethite, akaganeite, schwertmannite, and maghemite are leading candidates for the origin of the absorption centered near 900 nm in IMP spectra. The ferrous component in the soil cannot be well-constrained based on IMP data. Alpha proton X-ray spectrometer (APXS) measurements of six soil units show little variability within the landing site and show remarkable overall similarity to the average Viking-derived soil elemental composition. Differences exist between Viking and Pathfinder soils, however, including significantly higher S and Cl abundances and lower Si abundances in Viking soils and the lack of a correlation between Ti and Fe in Pathfinder soils. No significant linear correlations were observed between IMP spectral properties and APXS elemental chemistry. Attempts at constraining the mineralogy of soils and dust using normative calculations involving mixtures of smectites and silicate and oxide minerals did not yield physically acceptable solutions. We attempted to use the Pathfinder results to constrain a number of putative soil and dust formation scenarios, including palagonitization and acid-fog weathering. While the Pathfinder soils cannot be chemically linked to the Pathfinder rocks by palagonitization, this study and McSween et al. [1999] suggest that palagonitic alteration of a Martian basaltic rock, plus mixture with a minor component of locally derived andesitic rock fragments, could be consistent with the observed soil APXS and IMP properties.

Digital mapping of the Mars Pathfinder landing site: Design, acquisition, and derivation of cartographic products for science applications

USGS Publications Warehouse

Gaddis, L.R.; Kirk, R.L.; Johnson, J. R.; Soderblom, L.A.; Ward, A.W.; Barrett, J.; Becker, K.; Decker, T.; Blue, J.; Cook, D.; Eliason, E.; Hare, T.; Howington-Kraus, E.; Isbell, C.; Lee, E.M.; Redding, B.; Sucharski, R.; Sucharski, T.; Smith, P.H.; Britt, D.T.

1999-01-01

The Imager for Mars Pathfinder (IMP) acquired more than 16,000 images and provided panoramic views of the surface of Mars at the Mars Pathfinder landing site in Ares Vallis. This paper describes the stereoscopic, multispectral IMP imaging sequences and focuses on their use for digital mapping of the landing site and for deriving cartographic products to support science applications of these data. Two-dimensional cartographic processing of IMP data, as performed via techniques and specialized software developed for ISIS (the U.S.Geological Survey image processing software package), is emphasized. Cartographic processing of IMP data includes ingestion, radiometric correction, establishment of geometric control, coregistration of multiple bands, reprojection, and mosaicking. Photogrammetric processing, an integral part of this cartographic work which utilizes the three-dimensional character of the IMP data, supplements standard processing with geometric control and topographic information [Kirk et al., this issue]. Both cartographic and photogrammetric processing are required for producing seamless image mosaics and for coregistering the multispectral IMP data. Final, controlled IMP cartographic products include spectral cubes, panoramic (360?? azimuthal coverage) and planimetric (top view) maps, and topographic data, to be archived on four CD-ROM volumes. Uncontrolled and semicontrolled versions of these products were used to support geologic characterization of the landing site during the nominal and extended missions. Controlled products have allowed determination of the topography of the landing site and environs out to ???60 m, and these data have been used to unravel the history of large- and small-scale geologic processes which shaped the observed landing site. We conclude by summarizing several lessons learned from cartographic processing of IMP data. Copyright 1999 by the American Geophysical Union.

Sojourner APXS at Shark

NASA Image and Video Library

1997-08-28

NASA's Sojourner rover is seen next to the rock "Shark," in this image taken by the Imager for Mars Pathfinder (IMP) near the end of daytime operations on Sol 52. The rover's Alpha Proton X-Ray Spectrometer is deployed against the rock. The rock "Wedge" is in the foreground. The Sojourner rover is seen next to the rock "Shark," in this image taken by the Imager for Mars Pathfinder (IMP) near the end of daytime operations on Sol 52. The rover's Alpha Proton X-Ray Spectrometer is deployed against the rock. The rock "Wedge" is in the foreground.

Barnacle Bill in Super Resolution from Insurance Panorama

NASA Technical Reports Server (NTRS)

1998-01-01

Barnacle Bill is a small rock immediately west-northwest of the Mars Pathfinder lander and was the first rock visited by the Sojourner Rover's alpha proton X-ray spectrometer (APXS) instrument. This image shows super resolution techniques applied to the first APXS target rock, which was never imaged with the rover's forward cameras. Super resolution was applied to help to address questions about the texture of this rock and what it might tell us about its mode of origin.

This view of Barnacle Bill was produced by combining the 'Insurance Pan' frames taken while the IMP camera was still in its stowed position on sol2. The composite color frames that make up this anaglyph were produced for both the right and left eye of the IMP. The right eye composite consists of 5 frames, taken with different color filters, the left eye consists of only 1 frame. The resultant image from each eye was enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be. These panchromatic frames were then colorized with the red, green, and blue filtered images from the same sequence. The color balance was adjusted to approximate the true color of Mars.

The anaglyph view was produced by combining the left with the right eye color composite frames by assigning the left eye composite view to the red color plane and the right eye composite view to the green and blue color planes (cyan), to produce a stereo anaglyph mosaic. This mosaic can be viewed in 3-D on your computer monitor or in color print form by wearing red-blue 3-D glasses.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. Barnacle Bill is a small rock immediately west-northwest of the Mars Pathfinder lander and was the first rock visited by the Sojourner Rover's alpha proton X-ray spectrometer (APXS) instrument.

Sunset over Twin Peaks

NASA Technical Reports Server (NTRS)

1997-01-01

This image was taken by the Imager for Mars Pathfinder (IMP) about one minute after sunset on Mars on Sol 21. The prominent hills dubbed 'Twin Peaks' form a dark silhouette at the horizon, while the setting sun casts a pink glow over the darkening sky. The image was taken as part of a twilight study which indicates how the brightness of the sky fades with time after sunset. Scientists found that the sky stays bright for up to two hours after sunset, indicating that Martian dust extends very high into the atmosphere.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Pre-Dawn Martian Sky

NASA Technical Reports Server (NTRS)

1997-01-01

On Sol 39 there were wispy blue clouds in the pre-dawn sky of Mars, as seen by the Imager for Mars Pathfinder (IMP). The color image was made by taking blue, green, and red images and then combining them into a single color image. The clouds appear to have a bluish side and a greenish side because they moved (in the wind from the northeast) between images. This picture was made an hour and twenty minutes before sunrise -- the sun is not shining directly on the water ice clouds, but they are illuminated by the dawn twilight.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Yogi the rock - 3D

NASA Technical Reports Server (NTRS)

1997-01-01

Yogi, a rock taller than rover Sojourner, is the subject of this image, taken in stereo by the deployed Imager for Mars Pathfinder (IMP) on Sol 3. 3D glasses are necessary to identify surface detail. The soil in the foreground has been the location of multiple soil mechanics experiments performed by Sojourner's cleated wheels. Pathfinder scientists were able to control the force inflicted on the soil beneath the rover's wheels, giving them insight into the soil's mechanical properties. The soil mechanics experiments were conducted after this image was taken.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

Pathfinder Rear Ramp

NASA Image and Video Library

1997-07-06

NASA's Mars Pathfinder's rear rover ramp can be seen successfully unfurled in this image, taken at the end of Sol 2 by the Imager for Mars Pathfinder (IMP). This ramp was later used for the deployment of the microrover Sojourner, which occurred at the end of Sol 2. Areas of a lander petal and deflated airbag are visible at left. The image helped Pathfinder scientists determine that the rear ramp was the one to use for rover deployment. At upper right is the rock dubbed "Barnacle Bill," which Sojourner will later study. http://photojournal.jpl.nasa.gov/catalog/PIA00627

Ventifacts at the Pathfinder landing site

USGS Publications Warehouse

Bridges, N.T.; Greeley, R.; Haldemann, A.F.C.; Herkenhoff, K. E.; Kraft, M.; Parker, T.J.; Ward, A.W.

1999-01-01

About half of the rocks at the Mars Pathfinder Ares Vallis landing site appear to be ventifacts, rocks abraded by windborne particles. Comparable resolution images taken by the Imager for Mars Pathfinder (IMP) camera and the Viking landers show that ventifacts are more abundant at the Pathfinder site. The ventifacts occur in several forms, including rocks with faceted edges, finger-like projections, elongated pits, flutes, grooves, and possible rills. The trends of elongated pits, flutes, grooves, and rills cluster at ???280-330?? clockwise from north and generally dip 10-30?? away from their trend direction. These orientations are indicative of southeast to northwest winds and differ from the trend of wind tails at the landing site, the direction of local wind streaks, and predictions of the Global Circulation Model, all of which indicate northeast to southwest winds. The disparity between these data sets strongly suggests that local circulation patterns have changed since the abrasion of the ventifacted rocks. The greater number of ventifacts at the Pathfinder site compared to either of the Viking sites is most easily explained as being due to a larger supply of abrading particles, composed of either sand-sized grains or indurated dust aggregates, and higher surface roughness, which should increase the momentum of saltating grains. The Pathfinder ventifacts may have formed shortly after the deposition of outflow channel sediments nearly 2 Gry ago, when a large local supply of abrading particles should have been abundant and atmospheric conditions may have been more conducive to rock abrasion from saltating grains. Based on how ventifacts form on Earth, the several ventifact forms seen at the Pathfinder site and their presence on some rocks but not on others are probably due to local airflow conditions, original rock shape, exposure duration, rock movement, and to a lesser extent, rock lithology. The abundance of ventifacts at the Pathfinder site, together with other evidence of weathering, indicates that unaltered rock surfaces are rare on Mars. Copyright 1999 by the American Geophysical Union.

Big Crater as Viewed by Pathfinder Lander - Anaglyph

NASA Technical Reports Server (NTRS)

1997-01-01

The 'Big Crater' is actually a relatively small Martian crater to the southeast of the Mars Pathfinder landing site. It is 1500 meters (4900 feet) in diameter, or about the same size as Meteor Crater in Arizona. Superimposed on the rim of Big Crater (the central part of the rim as seen here) is a smaller crater nicknamed 'Rimshot Crater.' The distance to this smaller crater, and the nearest portion of the rim of Big Crater, is 2200 meters (7200 feet). To the right of Big Crater, south from the spacecraft, almost lost in the atmospheric dust 'haze,' is the large streamlined mountain nicknamed 'Far Knob.' This mountain is over 450 meters (1480 feet) tall, and is over 30 kilometers (19 miles) from the spacecraft. Another, smaller and closer knob, nicknamed 'Southeast Knob' can be seen as a triangular peak to the left of the flanks of the Big Crater rim. This knob is 21 kilometers (13 miles) southeast from the spacecraft.

The larger features visible in this scene - Big Crater, Far Knob, and Southeast Knob - were discovered on the first panoramas taken by the IMP camera on the 4th of July, 1997, and subsequently identified in Viking Orbiter images taken over 20 years ago. The scene includes rocky ridges and swales or 'hummocks' of flood debris that range from a few tens of meters away from the lander to the distance of South Twin Peak. The largest rock in the nearfield, just left of center in the foreground, nicknamed 'Otter', is about 1.5 meters (4.9 feet) long and 10 meters (33 feet) from the spacecraft.

This view of Big Crater was produced by combining 6 individual 'Superpan' scenes from the left and right eyes of the IMP camera. Each frame consists of 8 individual frames (left eye) and 7 frames (right eye) taken with different color filters that were enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be.

The anaglyph view of Big Crater was produced by combining the left and right eye mosaics (above) by assigning the left eye view to the red color plane and the right eye view to the green and blue color planes (cyan), to produce a stereo anaglyph mosaic. This mosaic can be viewed in 3-D on your computer monitor or in color print form by wearing red-blue 3-D glasses.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The IMP was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

Multispectral Imaging from Mars PATHFINDER

NASA Technical Reports Server (NTRS)

Ferrand, William H.; Bell, James F., III; Johnson, Jeffrey R.; Bishop, Janice L.; Morris, Richard V.

2007-01-01

The Imager for Mars Pathfinder (IMP) was a mast-mounted instrument on the Mars Pathfinder lander which landed on Mars Ares Vallis floodplain on July 4, 1997. During the 83 sols of Mars Pathfinders landed operations, the IMP collected over 16,600 images. Multispectral images were collected using twelve narrowband filters at wavelengths between 400 and 1000 nm in the visible and near infrared (VNIR) range. The IMP provided VNIR spectra of the materials surrounding the lander including rocks, bright soils, dark soils, and atmospheric observations. During the primary mission, only a single primary rock spectral class, Gray Rock, was recognized; since then, Black Rock, has been identified. The Black Rock spectra have a stronger absorption at longer wavelengths than do Gray Rock spectra. A number of coated rocks have also been described, the Red and Maroon Rock classes, and perhaps indurated soils in the form of the Pink Rock class. A number of different soil types were also recognized with the primary ones being Bright Red Drift, Dark Soil, Brown Soil, and Disturbed Soil. Examination of spectral parameter plots indicated two trends which were interpreted as representing alteration products formed in at least two different environmental epochs of the Ares Vallis area. Subsequent analysis of the data and comparison with terrestrial analogs have supported the interpretation that the rock coatings provide evidence of earlier martian environments. However, the presence of relatively uncoated examples of the Gray and Black rock classes indicate that relatively unweathered materials can persist on the martian surface.

Location and Geologic Setting for the Three U.S. Mars Landers

NASA Technical Reports Server (NTRS)

Parker, T. J.; Kirk, R. L.

1999-01-01

Super resolution of the horizon at both Viking landing sites has revealed "new" features we use for triangulation, similar to the approach used during the Mars Pathfinder Mission. We propose alternative landing site locations for both landers for which we believe the confidence is very high. Super resolution of VL-1 images also reveals some of the drift material at the site to consist of gravel-size deposits. Since our proposed location for VL-2 is NOT on the Mie ejecta blanket, the blocky surface around the lander may represent the meter-scale texture of "smooth palins" in the region. The Viking Lander panchromatic images typically offer more repeat coverage than does the IMP on Mars Pathfinder, due to the longer duration of these landed missions. Sub-pixel offsets, necessary for super resolution to work, appear to be attributable to thermal effects on the lander and settling of the lander over time. Due to the greater repeat coverage (particularly in the near and mid-fields) and all-panchromatic images, the gain in resolution by super resolution processing is better for Viking than it is with most IMP image sequences. This enhances the study of textural details near the lander and enables the identification rock and surface textures at greater distances from the lander. Discernment of stereo in super resolution im-ages is possible to great distances from the lander, but is limited by the non-rotating baseline between the two cameras and the shorter height of the cameras above the ground compared to IMP. With super resolution, details of horizon features, such as blockiness and crater rim shapes, may be better correlated with Orbiter images. A number of horizon features - craters and ridges - were identified at VL-1 during the misison, and a few hils and subtle ridges were identified at VL-2. We have added a few "new" horizon features for triangulation at the VL-2 landing site in Utopia Planitia. These features were used for independent triangulation with features visible in Viking Orbiter and MGS MOC images, though the actual location of VL-1 lies in a data dropout in the MOC image of the area. Additional information is contained in the original extended abstract.

Super Resolution Image of Yogi

NASA Technical Reports Server (NTRS)

1997-01-01

Yogi is a meter-size rock about 5 meters northwest of the Mars Pathfinder lander and was the second rock visited by the Sojourner Rover's alpha proton X-ray spectrometer (APXS) instrument. This mosaic shows super resolution techniques applied to the second APXS target rock, which was poorly illuminated in the rover's forward camera view taken before the instrument was deployed. Super resolution was applied to help to address questions about the texture of this rock and what it might tell us about its mode of origin.

This mosaic of Yogi was produced by combining four 'Super Pan' frames taken with the IMP camera. This composite color mosaic consists of 7 frames from the right eye, taken with different color filters that were enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be. This panchromatic frame was then colorized with the red, green, and blue filtered images from the same sequence. The color balance was adjusted to approximate the true color of Mars. Shadows were processed separately from the rest of the rock and combined with the rest of the scene to bring out details in the shadow of Yogi that would be too dark to view at the same time as the sunlit surfaces.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

New perspective of undeployed rover

NASA Technical Reports Server (NTRS)

1997-01-01

This image features a different perspective of one of the first pictures taken by the Imager for Mars Pathfinder (IMP) lander shortly after its touchdown at 10:07 AM Pacific Daylight Time on July 4. The image has been transformed to a perspective that would match that of an observer standing at the point the image was taken. Sojourner is seen in the foreground in its stowed position on a solar panel of the lander. Both ramps, the rear of which Sojourner would use on July 5 to safely descend to the Martian surface, were still undeployed when this image was taken. The double hills called 'Twin Peaks' are clearly visible in the background.

The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Airbag retraction

NASA Technical Reports Server (NTRS)

1997-01-01

This image shows that the Mars Pathfinder airbags have been successfully retracted, allowing safe deployment of the rover ramps. The Sojourner rover is at lower right, and rocks are visible in the background. Mars Pathfinder landed successfully on the surface of Mars today at 10:07 a.m. PDT.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

360-degree b/w Monster

NASA Image and Video Library

1997-07-10

This 360 degree "monster" panorama was taken by the deployed Imager for Mars Pathfinder (IMP) on Sol 3. All three petals, the perimeter of the deflated airbags, deployed rover Sojourner, forward and backward ramps and prominent surface features are visible. The IMP stands 1.8 meters over the Martian surface. The curvature and misalignment of several sections are due to image parallax. http://photojournal.jpl.nasa.gov/catalog/PIA00662

Airbag retraction

NASA Technical Reports Server (NTRS)

1997-01-01

This image shows that the Mars Pathfinder airbags have been successfully retracted, allowing safe deployment of the rover ramps. The Sojourner rover, still in its deployed position, is at center image, and rocks are visible in the background. Mars Pathfinder landed successfully on the surface of Mars today at 10:07 a.m. PDT.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

"Roadrunner Flats"

NASA Image and Video Library

1997-10-14

This enhanced color image of the Pathfinder landing site shows the eastern horizon. The elongated, reddish, low contrast region in the distance is "Roadrunner Flats." This image was taken by the Imager for Mars Pathfinder (IMP). Sojourner spent 83 days of a planned seven-day mission exploring the Martian terrain, acquiring images, and taking chemical, atmospheric and other measurements. The final data transmission received from Pathfinder was at 10:23 UTC on September 27, 1997. Although mission managers tried to restore full communications during the following five months, the successful mission was terminated on March 10, 1998. http://photojournal.jpl.nasa.gov/catalog/PIA00979

Results of the Imager for Mars Pathfinder windsock experiment

USGS Publications Warehouse

Sullivan, R.; Greeley, R.; Kraft, M.; Wilson, G.; Golombek, M.; Herkenhoff, K.; Murphy, J.; Smith, P.

2000-01-01

The Imager for Mars Pathfinder (IMP) windsock experiment measured wind speeds at three heights within 1.2 m of the Martian surface during Pathfinder landed operations. These wind data allowed direct measurement of near-surface wind profiles on Mars for the first time, including determination of aerodynamic roughness length and wind friction speeds. Winds were light during periods of windsock imaging, but data from the strongest breezes indicate aerodynamic roughness length of 3 cm at the landing site, with wind friction speeds reaching 1 m/s. Maximum wind friction speeds were about half of the threshold-of-motion friction speeds predicted for loose, fine-grained materials on smooth Martian terrain and about one third of the threshold-of-motion friction speeds predicted for the same size particles over terrain with aerodynamic roughness of 3 cm. Consistent with this, and suggesting that low wind speeds prevailed when the windsock array was not imaged and/or no particles were available for aeolian transport, no wind-related changes to the surface during mission operations have been recognized. The aerodynamic roughness length reported here implies that proposed deflation of fine particles around the landing site, or activation of duneforms seen by IMP and Sojourner, would require wind speeds >28 m/s at the Pathfinder top windsock height (or >31 m/s at the equivalent Viking wind sensor height of 1.6 m) and wind speeds >45 m/s above 10 m. These wind speeds would cause rock abrasion if a supply of durable particles were available for saltation. Previous analyses indicate that the Pathfinder landing site probably is rockier and rougher than many other plains units on Mars, so aerodynamic roughness length elsewhere probably is less than the 3-cm value reported for the Pathfinder site. Copyright 2000 by the American Geophysical Union.

Panoramic Views of the Landing site from Sagan Memorial Station

NASA Technical Reports Server (NTRS)

1997-01-01

Each of these panoramic views is a controlled mosaic of approximately 300 IMP images covering 360 degrees of azimuth and elevations from approximately 4 degrees above the horizon to 45 degrees below it. Simultaneous adjustment of orientations of all images has been performed to minimize discontinuities between images. Mosaics have been highpass-filtered and contrast-enhanced to improve discrimination of details without distorting relative colors overall.

TOP IMAGE: Enhanced true-color image created from the 'Gallery Pan' sequence, acquired on sols 8-10 so that local solar time increases nearly continuously from about 10:00 at the right edge to about 12:00 at the left. Mosaics of images obtained by the right camera through 670 nm, 530 nm, and 440 nm filters were used as red, green and blue channels. Grid ticks indicate azimuth clockwise from north in 30 degree increments and elevation in 15 degree increments.

BOTTOM IMAGE: Anaglyphic stereoimage created from the 'monster pan' sequence, acquired in four sections between about 8:30 and 15:00 local solar time on sol 3. Mosaics of images obtained through the 670 nm filter (left camera) and 530 and 440 nm filters (right camera) were used where available. At the top and bottom, left- and right-camera 670 nm images were used. Part of the northern horizon was not imaged because of the tilt of the lander. This image may be viewed stereoscopically through glasses with a red filter for the left eye and a cyan filter for the right eye.

NOTE: original caption as published in Science Magazine

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Overhead View of Pathfinder Landing Site

NASA Technical Reports Server (NTRS)

1997-01-01

Planimetric (overhead view) map of the landing site, to a distance of 20 meters from the spacecraft. North is at the top in this and Plates 3-5. To produce this map, images were geometrically projected onto an assumed mean surface representing the ground. Features above the ground plane (primarily rocks) therefore appear displaced radially outward; the amount of distortion increases systematically with distance. The upper surfaces of the lander and rover also appear enlarged and displaced because of their height. Primary grid (white) is based on the Landing Site Cartographic (LSC) coordinate system, defined with X eastward, Y north, and Z up, and origin located at the mean ground surface immediately beneath the deployed position of the IMP camera gimbal center. Secondary ticks (cyan) are based on the Mars local level (LL) frame, which has X north, Y east, Z down, with origin in the center of the lander baseplate. Rover positions (including APXS measurements) are commonly reported in the LL frame. Yellow grid shows polar coordinates based on the LSC system. Cartographic image processing by U.S. Geological Survey.

NOTE: original caption as published in Science Magazine

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Lander, Airbags, & Martian terrain

NASA Technical Reports Server (NTRS)

1997-01-01

Several objects have been imaged by the Imager for Mars Pathfinder (IMP) during the spacecraft's first day on Mars. Portions of the deflated airbags, part of one the lander's petals, soil, and several rocks are visible. The furrows in the soil were artificially produced by the retraction of the airbags after landing, which occurred at 10:07 a.m. PDT.

The IMP is a stereo imaging system with color capability provided by 24 selectable filters -- twelve filters per 'eye.

Sojourner Sits Near Rock Garden

NASA Technical Reports Server (NTRS)

2003-01-01

The Mars Pathfinder Rover Sojourner is images by the Imager for Mars Pathfinder as it nears the rock 'Wedge.' Part of the Rock Garden is visible in the upper right of the image.

Pathfinder, a low-cost Discovery mission, is the first of a new fleet of spacecraft that are planned to explore Mars over the next ten years. Mars Global Surveyor, already en route, arrives at Mars on September 11 to begin a two year orbital reconnaissance of the planet's composition, topography, and climate. Additional orbiters and landers will follow every 26 months.

The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Rover Soil Experiments Near Yogi

NASA Technical Reports Server (NTRS)

1997-01-01

Sojourner, while on its way to the rock Yogi, performed several soil mechanics experiments. Piles of loose material churned up from the experiment are seen in front of and behind the Rover. The rock Pop-Tart is visible near the front right rover wheel. Yogi is at upper right. The image was taken by the Imager for Mars Pathfinder.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Northeast View from Pathfinder Lander

NASA Image and Video Library

1997-11-04

This panorama of the region to the northeast of the lander was constructed to support the Sojourner Rover Team's plans to conduct an "autonomous traverse" to explore the terrain away from the lander after science objectives in the lander vicinity had been met. The large, relatively bright surface in the foreground, about 10 meters (33 feet) from the spacecraft, in this scene is "Baker's Bench." The large, elongated rock left of center in the middle distance is "Zaphod." This view was produced by combining 8 individual "Superpan" scenes from the left and right eyes of the IMP camera. Each frame consists of 8 individual frames (left eye) and 7 frames (right eye) taken with different color filters that were enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be. http://photojournal.jpl.nasa.gov/catalog/PIA01000

Photogrammetric analysis of horizon panoramas: The Pathfinder landing site in Viking orbiter images

USGS Publications Warehouse

Oberst, J.; Jaumann, R.; Zeitler, W.; Hauber, E.; Kuschel, M.; Parker, T.; Golombek, M.; Malin, M.; Soderblom, L.

1999-01-01

Tiepoint measurements, block adjustment techniques, and sunrise/sunset pictures were used to obtain precise pointing data with respect to north for a set of 33 IMP horizon images. Azimuth angles for five prominent topographic features seen at the horizon were measured and correlated with locations of these features in Viking orbiter images. Based on this analysis, the Pathfinder line/sample coordinates in two raw Viking images were determined with approximate errors of 1 pixel, or 40 m. Identification of the Pathfinder location in orbit imagery yields geological context for surface studies of the landing site. Furthermore, the precise determination of coordinates in images together with the known planet-fixed coordinates of the lander make the Pathfinder landing site the most important anchor point in current control point networks of Mars. Copyright 1999 by the American Geophysical Union.

First image of clouds over Mars

NASA Technical Reports Server (NTRS)

1997-01-01

This is the first image ever taken from the surface of Mars of an overcast sky. Featured are stratus clouds coming from the northeast at about 15 miles per hour (6.7 meters/second) at an approximate height of ten miles (16 kilometers) above the surface. The 'you are here' notation marks where Earth was situated in the sky at the time the image was taken. Scientists had hoped to see Earth in this image, but the cloudy conditions prevented a clear viewing. Similar images will be taken in the future with the hope of capturing a view of Earth. From Mars, Earth would appear as a tiny blue dot as a star would appear to an Earthbound observer. Pathfinder's imaging system will not be able to resolve Earth's moon. The clouds consist of water ice condensed on reddish dust particles suspended in the atmosphere. Clouds on Mars are sometimes localized and can sometimes cover entire regions, but have not yet been observed to cover the entire planet. The image was taken about an hour and forty minutes before sunrise by the Imager for Mars Pathfinder (IMP) on Sol 16 at about ten degrees up from the eastern Martian horizon.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages and Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

MARS PATHFINDER CAMERA TEST IN SAEF-2

NASA Technical Reports Server (NTRS)

1996-01-01

In the Spacecraft Assembly and Encapsulation Facility-2 (SAEF-2), workers from the Jet Propulsion Laboratory (JPL) are conducting a systems test of the imager for the Mars Pathfinder. Mounted on the Pathfinder lander, the imager (the white cylindrical element the worker is touching) is a specially designed camera featuring a stereo-imaging system with color capability provided by a set of selectable filters. It is mounted on an extendable mast that will pop up after the lander touches down on the Martian surface. The imager will transmit images of the terrain, allowing engineers back on Earth to survey the landing site before the Pathfinder rover is deployed to explore the area. The Mars Pathfinder is scheduled for launch aboard a Delta II expendable launch vehicle on Dec. 2. JPL manages the Pathfinder project for NASA.

New Insights into the Geology of the Mars Pathfinder Landing Site from Spectral and Morphologic Analysis of the 12-Color Superpan Panorama

NASA Technical Reports Server (NTRS)

Murchie, S.; Barnouin-Jha, O.; Barnouin-Jha, K.; Bishop, J.; Johnson, J.; McSween, H.; Morris, R.

2003-01-01

New analyses of rocks and soils at the Mars Pathfinder landing site have been completed using the full Imager for Mars Pathfinder (IMP) 12- color SuperPan panorama. These revise early conclusions that rocks at the landing site are a single lithology coated only by windblown dust. We conclude instead that there is also a second lithology in addition to the dominant gray rock, and that it is consistent with highlands material excavated from beneath a thin veneer of northern plains; that many rocks have cemented coatings that formed during an early, probably wetter climate; and that young rocks excavated after coating formation ceased are mainly breccias or conglomerates.

Shark

NASA Technical Reports Server (NTRS)

1997-01-01

This false color composite image from the Pathfinder lander shows the rock 'Shark' at upper right (Shark is about 0.69 m wide, 0.40 m high, and 6.4 m from the lander). The rock looks like a conglomerate in Sojourner rover images, but only the large elements of its surface textures can be seen here. This demonstrates the usefulness of having a robot rover geologist able to examine rocks up close.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

First look at rock & soil properties

NASA Technical Reports Server (NTRS)

1997-01-01

The earliest survey of spectral properties of the rocks and soils surrounding Pathfinder was acquired as a narrow strip covering the region just beyond the where the rover made its egress from the lander. The wavelength filters used, all in the binocular camera's right eye, cover mainly visible wavelengths. These data reveal at least five kinds of rocks and soil in the immediate vicinity of the lander. All of the spectra are ratioed to the mean spectrum of bright red drift to highlight the differences. Different occurrences of drift (pink spectra) are closely similar. Most of the rocks (black spectra) have a dark gray color, and are both darker and less red than the drift, suggesting less weathering. Typical soils (green spectra) are intermediate in properties to the rocks and drift. Both these data and subsequent higher resolution images show that the typical soil consists of a mixture of drift and small dark gray particles resembling the rock. However, two other kinds of materials are significantly different from the rocks and drift. Pinkish or whitish pebbles and crusts on some of the rocks (blue spectra) are brighter in blue light and darker in near-infrared light than is the drift, and they lack the spectral characteristics closely associated with iron minerals. Dark red soils in the lee of several rocks are about as red as the drift, but consistently darker. The curvature in the spectrum at visible wavelengths suggests either more ferric iron minerals than in the drift or a larger particle size.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator. JPL is an operating division of the California Institute of Technology (Caltech).

Rock Garden

NASA Technical Reports Server (NTRS)

1997-01-01

This false color composite image of the Rock Garden shows the rocks 'Shark' and 'Half Dome' at upper left and middle, respectively. Between these two large rocks is a smaller rock (about 0.20 m wide, 0.10 m high, and 6.33 m from the Lander) that was observed close-up with the Sojourner rover (see PIA00989).

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Diversity of soils near rover deploy region

NASA Technical Reports Server (NTRS)

1997-01-01

The surface near the rover's egress from the lander contains mainly bright red drift (#1), dark gray rocks such as Cradle (# 3), soil intermediate in color to the rocks and drift (#2), and dark red soil on and around the rock Lamb (#4). Globally, Mars is characterized by similar color variations. The spectra, measured using the full 13-color capability of the Imager for Mars Pathfinder (IMP), provide evidence for the mineralogy of the unweathered rocks and highly weathered red soils.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech).

Marie Curie during ORT4

NASA Technical Reports Server (NTRS)

2003-01-01

Marie Curie rover drives down the rear ramp during Operational Readiness Test (ORT) 4.

Pathfinder, a low-cost Discovery mission, is the first of a new fleet of spacecraft that are planned to explore Mars over thenext ten years. Mars Global Surveyor, already en route, arrives at Mars on September 11 to begin a two year orbital reconnaissance of the planet's composition, topography, and climate. Additional orbiters and landers will follow every 26 months.

The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Mapping Rock and Soil Units in the MPF IMP SuperPan Using a Kohonen Self Organizing Map

NASA Technical Reports Server (NTRS)

Farrand, W.; Merenyi, E.; Murchie, S.; Barnouin-Jha, O.; Johnson, J.

2004-01-01

The 1997 Mars Pathfinder mission provided information on a site in the Ares Vallis floodplain. Initial analysis of multispectral data from the Imager for Mars Pathfinder (IMP) indicated the presence of only a single rock type, the 'gray rock' spectral class and various coated variants thereof (e.g., 'maroon rock'). Continued analysis of the IMP 'SuperPan' mosaic has confirmed multiple examples of a second 'black rock' spectral class existing as small cobbles in the near field and as boulders in the far field. These results are consistent with recent analysis of MGS Thermal Emission Spectrometer (TES) data which indicates that there is likely a mix of both 'Surface Type 1' (ST1) and 'Surface Type 2' (ST2) spectral classes at the MPF landing site. Nominally, the black rock spectral class would correspond to ST1 (basalts) and 'gray rock' would correspond to ST2 (andesites). Orbital remote sensing has also revealed the pervasive presence of layering on Mars. Recently it was suggested that there are extensive outcrops of the black rock spectral class in the SuperPan far field on the flanks of the Twin Peaks and on the rim of Big Crater. These authors suggested that these exposures represented outcrops of black rock from beneath a surficial, flood deposited layer. In this work, we have reexamined the MPF IMP SuperPan mosaic using an artificial neural network self organizing map (SOM) processing architecture in order to classify the distribution of spectral classes within the SuperPan. In this paper, we present initial results from that work and draw specific attention to a subset of the identified spectral classes in order to address questions relating to whether there are extensive exposures of black rock in the IMP far field, what other materials might be exposed in the far field, and what evidence there is for subsurface layering at the MPF landing site.

Wind effects on Martian soil

NASA Technical Reports Server (NTRS)

1997-01-01

This false-color combination image highlights details of wind effects on the Martian soil at the Pathfinder landing site. Red and blue filter images have been combined to enhance brightness contrasts among several soil units. Martian winds have distributed these lighter and darker fine materials in complex patterns around the rocks in the scene (blue). For scale, the rock at right center is 16 centimeters (6.3 inches) long. This scene is one of several that will be monitored weekly for changes caused by wind activity.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages and Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Soil disturbance by airbags

NASA Technical Reports Server (NTRS)

1997-01-01

Disturbance of the drift at the Pathfinder landing site reveals a shallow subsurface that is slightly darker but has similar spectral properties. The top set of images, in true color, shows the soils disturbed by the last bounce of the lander on its airbags before coming to rest and the marks created by retraction of the airbags. In the bottom set of images color differences have been enhanced. The mast at center is the Atmospheric Structure Instrument/Meteorology Package (ASI/MET). The ASI/MET is an engineering subsytem that acquired atmospheric data during Pathfinder's descent, and will continue to get more data through the entire landed mission. A shadow of the ASI/MET appears on a rock at left.

Mars Pathfinder was developed and managed by the Jet Propulsion Laboratory (JPL) for the National Aeronautics and Space Administration. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Color Mosaic of Rover & Terrain

NASA Image and Video Library

1997-07-05

NASA's Sojourner rover and undeployed ramps onboard the Mars Pathfinder spacecraft can be seen in this image, by the Imager for Mars Pathfinder (IMP) on July 4 (Sol 1). This image has been corrected for the curvature created by parallax. The microrover Sojourner is latched to the petal, and has not yet been deployed. The ramps are a pair of deployable metal reels which will provide a track for the rover as it slowly rolls off the lander, over the spacecraft's deflated airbags, and onto the surface of Mars. Pathfinder scientists will use this image to determine whether it is safe to deploy the ramps. One or both of the ramps will be unfurled, and then scientists will decide whether the rover will use either the forward or backward ramp for its descent. http://photojournal.jpl.nasa.gov/catalog/PIA00621

Sojourner near the Rock Garden

NASA Technical Reports Server (NTRS)

1997-01-01

This image of the Sojourner rover was taken near the end of daytime operations on Sol 42. The rover is between the rocks 'Wedge' (left) and 'Flute Top' (right). Other rocks visible include 'Flat Top' (behind Flute Top) and those in the Rock Garden, at the top of the frame. The cylindrical object extending from the back end of Sojourner is the Alpha Proton X-Ray Spectrometer.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Marie Curie during ORT6

NASA Technical Reports Server (NTRS)

2003-01-01

Marie Curie sits on the lander petal prior to deployment during the pre launch Operations Readiness Test (ORT) 6.

Pathfinder, a low-cost Discovery mission, is the first of a new fleet of spacecraft that are planned to explore Mars over thenext ten years. Mars Global Surveyor, already en route, arrives at Mars on September 11 to begin a two year orbital reconnaissance of the planet's composition, topography, and climate. Additional orbiters and landers will follow every 26 months.

The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

Terrain and Rock Yogi

NASA Image and Video Library

1997-07-06

The left portion of this image, taken by the Imager for Mars Pathfinder (IMP) on July 5, 1997 (Sol 2), shows a portion of the large rock nicknamed "Yogi." Portions of a petal and deflated airbag are in the foreground. The dark circular object at right is a portion of the lander's high-gain antenna. http://photojournal.jpl.nasa.gov/catalog/PIA00630

Software Aids Visualization Of Mars Pathfinder Mission

NASA Technical Reports Server (NTRS)

Weidner, Richard J.

1996-01-01

Report describes Simulator for Imager for Mars Pathfinder (SIMP) computer program. SIMP generates "virtual reality" display of view through video camera on Mars lander spacecraft of Mars Pathfinder mission, along with display of pertinent textual and graphical data, for use by scientific investigators in planning sequences of activities for mission.

Rover Touchdown on Martian Surface

NASA Image and Video Library

1997-07-06

This picture taken by the IMP (Imager for Mars Pathfinder) aboard the Mars Pathfinder spacecraft depicts the rover Sojourner's position after driving onto the Martian surface. Sojourner has become the first autonomous robot ever to traverse the surface of Mars. This image reflects the success of Pathfinder's principle objective -- to place a payload on Mars in a safe, operational configuration. The primary mission of Sojourner, scheduled to last seven days, will be to use its Alpha Proton X-ray Spectrometer (APXS) instrument to determine the elements that make up the rocks and soil on Mars. A full study using the APXS takes approximately ten hours, and can measure all elements except hydrogen at any time of the Martian day or night. The APXS will conduct its studies by bombarding rocks and soil samples with alpha particle radiation -- charged particles equivalent to the nucleus of a helium atom, consisting of two protons and two neutrons. http://photojournal.jpl.nasa.gov/catalog/PIA00623

Barnacle Bill in Super Resolution from Super Panorama

NASA Image and Video Library

1998-07-03

"Barnacle Bill" is a small rock immediately west-northwest of the Mars Pathfinder lander and was the first rock visited by the Sojourner Rover's alpha proton X-ray spectrometer (APXS) instrument. This image shows super resolution techniques applied to the first APXS target rock, which was never imaged with the rover's forward cameras. Super resolution was applied to help to address questions about the texture of this rock and what it might tell us about its mode of origin. This view of Barnacle Bill was produced by combining the "Super Panorama" frames from the IMP camera. Super resolution was applied to help to address questions about the texture of these rocks and what it might tell us about their mode of origin. The composite color frames that make up this anaglyph were produced for both the right and left eye of the IMP. The composites consist of 7 frames in the right eye and 8 frames in the left eye, taken with different color filters that were enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be. These panchromatic frames were then colorized with the red, green, and blue filtered images from the same sequence. The color balance was adjusted to approximate the true color of Mars. The anaglyph view was produced by combining the left with the right eye color composite frames by assigning the left eye composite view to the red color plane and the right eye composite view to the green and blue color planes (cyan), to produce a stereo anaglyph mosaic. This mosaic can be viewed in 3-D on your computer monitor or in color print form by wearing red-blue 3-D glasses. http://photojournal.jpl.nasa.gov/catalog/PIA01409

Lander, Airbags, & Martian Terrain

NASA Image and Video Library

1997-07-05

Several objects have been imaged by the Imager for Mars Pathfinder (IMP) during the spacecraft's first day on Mars. Portions of the deflated airbags, part of one the lander's petals, soil, and several rocks are visible. The furrows in the soil were artificially produced by the retraction of the airbags after landing, which occurred at 10:07 a.m. PDT. http://photojournal.jpl.nasa.gov/catalog/PIA00616

Sojourner APXS at Moe - Right Eye

NASA Image and Video Library

1997-10-13

The Sojourner rover's Alpha Proton X-ray Spectrometer (APXS) is shown deployed against the rock "Moe" on the afternoon of Sol 64 (September 7). The rocks to the left of Moe are "Shark" (left of Sojourner) and "Half Dome" (behind Sojourner). They were previously measured by the APXS. The image was taken by the Imager for Mars Pathfinder (IMP). http://photojournal.jpl.nasa.gov/catalog/PIA00966

Sojourner APXS at Moe - Left Eye

NASA Image and Video Library

1999-07-02

The Sojourner rover's Alpha Proton X-ray Spectrometer (APXS) is shown deployed against the rock "Moe" on the afternoon of Sol 64 (September 7). The rocks to the left of Moe are "Shark" (left of Sojourner) and "Half Dome" (behind Sojourner). They were previously measured by the APXS. The image was taken by the Imager for Mars Pathfinder (IMP). Sojourner spent 83 days of a planned seven-day mission exploring the Martian terrain, acquiring images, and taking chemical, atmospheric and other measurements. The final data transmission received from Pathfinder was at 10:23 UTC on September 27, 1997. Although mission managers tried to restore full communications during the following five months, the successful mission was terminated on March 10, 1998. http://photojournal.jpl.nasa.gov/catalog/PIA01560

MARS PATHFINDER CAMERA TEST IN SAEF-2

NASA Technical Reports Server (NTRS)

1996-01-01

In the Spacecraft Assembly and Encapsulation Facility-2 (SAEF-2), workers from the Jet Propulsion Laboratory (JPL) are conducting a systems test of the imager for the Mars Pathfinder. The imager (white and metallic cylindrical element close to hand of worker at left) is a specially designed camera featuring a stereo- imaging system with color capability provided by a set of selectable filters. It is mounted atop an extendable mast on the Pathfinder lander. Visible to the far left is the small rover which will be deployed from the lander to explore the Martian surface. Transmitting back to Earth images of the trail left by the rover will be one of the mission objectives for the imager. To the left of the worker standing near the imager is the mast for the low-gain antenna; the round high-gain antenna is to the right. Visible in the background is the cruise stage that will carry the Pathfinder on a direct trajectory to Mars. The Mars Pathfinder is one of two Mars-bound spacecraft slated for launch aboard Delta II expendable launch vehicles this year.

I-123 amphetamine vs Xe-133 SPECT: A comparative study in patients with unilateral Cerebrovascular Disease (CVD)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buell, U.; Krappel, W.; Schmiedek, P.

1985-05-01

SPECT may be used to measure regional cerebral blood flow (rCBF) by X) Xe-133 gas and a rotating four detector array system or by I) I-123 amphetamine (IMP) and a rotating gamma camera. Results of X) and I) were compared to find out an optimum time frame wherein cerebral IMP distribution reflects rCBF, using Xe-133 as reference. In 20 patients (pts) with strictly unilateral CFD, X) and I) were performed within 48 hrs. X) was used to establish interhemispherical ratios (R) of rCBF (diseased-to-normal hemisphere) from both, hemispherical (half) slices (S, at 6-8 and 10-12 cm above OML, 2 SRmore » per pt) and standardized areas (A, 6 AR per S). I) was done with a dual head gamma camera at (2) 13-27, (2) 33-47 min and (3) 5.5. hrs after injection of 180 MBq IMP-123 (p,5n). After reconstruction, identical S of X) and I) were evaluated by a ROI computer program. For comparison, values of SR and AR were subtracted from l.000 and pronounced CVD was selected by thresholds for XE-R (>.11 and >.14). In pronounced CVD, IMP(2)SR was significantly different from Xe SR. AR showed a low correlation, too. At 5.5 hrs, IMP 3 did represent rCBF at least. However, excellent congruence was found at IMP 1 (13-27 min pi.) The authors conclude that in CVED cerebral IMP uptake and distribution represent rCBF only during the first 30 min after injection.« less

Interannual, seasonal and diurnal Mars surface environmental cycles observed from Viking to Curiosity

NASA Astrophysics Data System (ADS)

Martinez, German; Vicente-Retortillo, Álvaro; Kemppinen, Osku; Fischer, Erik; Fairen, Alberto G.; Guzewich, Scott David; Haberle, Robert; Lemmon, Mark T.; Newman, Claire E.; Renno, Nilton O.; Richardson, Mark I.; Smith, Michael D.; De la Torre, Manuel; Vasavada, Ashwin R.

2016-10-01

We analyze in-situ environmental data from the Viking landers to the Curiosity rover to estimate atmospheric pressure, near-surface air and ground temperature, relative humidity, wind speed and dust opacity with the highest confidence possible. We study the interannual, seasonal and diurnal variability of these quantities at the various landing sites over a span of more than twenty Martian years to characterize the climate on Mars and its variability. Additionally, we characterize the radiative environment at the various landing sites by estimating the daily UV irradiation (also called insolation and defined as the total amount of solar UV energy received on flat surface during one sol) and by analyzing its interannual and seasonal variability.In this study we use measurements conducted by the Viking Meteorology Instrument System (VMIS) and Viking lander camera onboard the Viking landers (VL); the Atmospheric Structure Instrument/Meteorology (ASIMET) package and the Imager for Mars Pathfinder (IMP) onboard the Mars Pathfinder (MPF) lander; the Miniature Thermal Emission Spectrometer (Mini-TES) and Pancam instruments onboard the Mars Exploration Rovers (MER); the Meteorological Station (MET), Thermal Electrical Conductivity Probe (TECP) and Phoenix Surface Stereo Imager (SSI) onboard the Phoenix (PHX) lander; and the Rover Environmental Monitoring Station (REMS) and Mastcam instrument onboard the Mars Science Laboratory (MSL) rover.A thorough analysis of in-situ environmental data from past and present missions is important to aid in the selection of the Mars 2020 landing site. We plan to extend our analysis of Mars surface environmental cycles by using upcoming data from the Temperature and Wind sensors (TWINS) instrument onboard the InSight mission and the Mars Environmental Dynamics Analyzer (MEDA) instrument onboard the Mars 2020 mission.

MARS PATHFINDER CAMERA TEST IN SAEF-2

NASA Technical Reports Server (NTRS)

1996-01-01

Jet Propulsion Laboratory (JPL) workers conduct a systems test of the Mars Pathfinder imager, installed atop the Pathfinder lander (with JPL insignia). The imager is the white cyclindrical structure close to the worker's gloved hand. At left is the small rover that will be deployed from the lander to explore the Martian surface. The rover is mounted on one of three petals that will be attached to the lander. The two-pronged mast extending upward from the lander is for the low-gain antenna. The imager is mounted on a mast that will be extended after the lander touches down on Mars, affording a better view of the area. The imager is a camera that will transmit images of the Martian surface as well as the trail left by the rover, helping researchers to better understand the composition of the soil. It also is equipped with selectable filters for gathering data about the atmosphere of the Red Planet. JPL manages the Mars Pathfinder project for NASA. The journey to Mars is scheduled to begin with liftoff Dec. 2 aboard a Delta II expendable launch vehicle.

Sojourner & Terrain

NASA Image and Video Library

1997-07-06

The undeployed Sojourner rover is seen still latched to a lander petal in this image, taken by the Imager for Mars Pathfinder (IMP) on Sol 1, the lander's first day on Mars. Portions of a petal and deflated airbag are in the foreground. The rectangular rock at right has been dubbed "Flat top," and may be a possible object of study for Sojourner's Alpha Proton X-Ray Spectrometer (APXS) instrument. The mismatched portion of image at left is a misregistered section of data. http://photojournal.jpl.nasa.gov/catalog/PIA00631

Rover, Airbags, & Surrounding Rocks

NASA Image and Video Library

1997-07-05

This image of the Martian surface was taken by the Imager for Mars Pathfinder (IMP) before sunset on July 4, 1997 (Sol 1), the spacecraft's first day on Mars. The airbags have been partially retracted, and portions the petal holding the undeployed rover Sojourner can be seen at lower left. The rock in the center of the image may be a future target for chemical analysis. The soil in the foreground has been disturbed by the movement of the airbags as they retracted. http://photojournal.jpl.nasa.gov/catalog/PIA00619

The color of the Martian sky and its influence on the illumination of the Martian surface

USGS Publications Warehouse

Thomas, N.; Markiewicz, W.J.; Sablotny, R.M.; Wuttke, M.W.; Keller, H.U.; Johnson, J. R.; Reid, R.J.; Smith, R.H.

1999-01-01

The dust in the atmosphere above the Mars Pathfinder landing site produced a bright, red sky that increases in redness toward the horizon at midday. There is also evidence for an absorption band in the scattered light from the sky at 860 nm. A model of the sky brightness has been developed [Markiewicz et al., this issue] and tested against Imager for Mars Pathfinder (IMP) observations of calibration targets on the lander. The resulting model has been used to quantify the total diffuse flux onto a surface parallel to the local level for several solar elevation angles and optical depths. The model shows that the diffuse illumination in shadowed areas is strongly reddened while areas illuminated directly by the Sun (and the blue forward scattering peak) see a more solar-type spectrum, in agreement with Viking and IMP observations. Quantitative corrections for the reddening in shadowed areas are demonstrated. It is shown quantitatively that the unusual appearance of the rock Yogi (the east face of which appeared relatively blue in images taken during the morning but relatively red during the afternoon) can be explained purely by the changing illumination geometry. We conclude that any spectrophotometric analysis of surfaces on Mars must take into account the diffuse flux. Specifically, the reflectances of surfaces viewed under different illumination geometries cannot be investigated for spectral diversity unless a correction has been applied which removes the influence of the reddened diffuse flux. Copyright 1999 by the American Geophysical Union.

Rover imaging system for the Mars rover/sample return mission

NASA Technical Reports Server (NTRS)

1993-01-01

In the past year, the conceptual design of a panoramic imager for the Mars Environmental Survey (MESUR) Pathfinder was finished. A prototype camera was built and its performace in the laboratory was tested. The performance of this camera was excellent. Based on this work, we have recently proposed a small, lightweight, rugged, and highly capable Mars Surface Imager (MSI) instrument for the MESUR Pathfinder mission. A key aspect of our approach to optimization of the MSI design is that we treat image gathering, coding, and restoration as a whole, rather than as separate and independent tasks. Our approach leads to higher image quality, especially in the representation of fine detail with good contrast and clarity, without increasing either the complexity of the camera or the amount of data transmission. We have made significant progress over the past year in both the overall MSI system design and in the detailed design of the MSI optics. We have taken a simple panoramic camera and have upgraded it substantially to become a prototype of the MSI flight instrument. The most recent version of the camera utilizes miniature wide-angle optics that image directly onto a 3-color, 2096-element CCD line array. There are several data-taking modes, providing resolution as high as 0.3 mrad/pixel. Analysis tasks that were performed or that are underway with the test data from the prototype camera include the following: construction of 3-D models of imaged scenes from stereo data, first for controlled scenes and later for field scenes; and checks on geometric fidelity, including alignment errors, mast vibration, and oscillation in the drive system. We have outlined a number of tasks planned for Fiscal Year '93 in order to prepare us for submission of a flight instrument proposal for MESUR Pathfinder.

Using virtual reality for science mission planning: A Mars Pathfinder case

NASA Technical Reports Server (NTRS)

Kim, Jacqueline H.; Weidner, Richard J.; Sacks, Allan L.

1994-01-01

NASA's Mars Pathfinder Project requires a Ground Data System (GDS) that supports both engineering and scientific payloads with reduced mission operations staffing, and short planning schedules. Also, successful surface operation of the lander camera requires efficient mission planning and accurate pointing of the camera. To meet these challenges, a new software strategy that integrates virtual reality technology with existing navigational ancillary information and image processing capabilities. The result is an interactive workstation based applications software that provides a high resolution, 3-dimensial, stereo display of Mars as if it were viewed through the lander camera. The design, implementation strategy and parametric specification phases for the development of this software were completed, and the prototype tested. When completed, the software will allow scientists and mission planners to access simulated and actual scenes of Mars' surface. The perspective from the lander camera will enable scientists to plan activities more accurately and completely. The application will also support the sequence and command generation process and will allow testing and verification of camera pointing commands via simulation.

Preliminary results on photometric properties of materials at the Sagan Memorial Station, Mars

USGS Publications Warehouse

Johnson, J. R.; Kirk, R.; Soderblom, L.A.; Gaddis, L.; Reid, R.J.; Britt, D.T.; Smith, P.; Lemmon, M.; Thomas, N.; Bell, J.F.; Bridges, N.T.; Anderson, R.; Herkenhoff, K. E.; Maki, J.; Murchie, S.; Dummel, A.; Jaumann, R.; Trauthan, F.; Arnold, G.

1999-01-01

Reflectance measurements of selected rocks and soils over a wide range of illumination geometries obtained by the Imager for Mars Pathfinder (IMP) camera provide constraints on interpretations of the physical and mineralogical nature of geologic materials at the landing site. The data sets consist of (1) three small "photometric spot" subframed scenes, covering phase angles from 20?? to 150??; (2) two image strips composed of three subframed images each, located along the antisunrise and antisunset lines (photometric equator), covering phase angles from ???0?? to 155??; and (3) full-image scenes of the rock "Yogi," covering phase angles from 48?? to 100??. Phase functions extracted from calibrated data exhibit a dominantly backscattering photometric function, consistent with the results from the Viking lander cameras. However, forward scattering behavior does appear at phase angles >140??, particularly for the darker gray rock surfaces. Preliminary efforts using a Hapke scattering model are useful in comparing surface properties of different rock and soil types but are not well constrained, possibly due to the incomplete phase angle availability, uncertainties related to the photometric function of the calibration targets, and/or the competing effects of diffuse and direct lighting. Preliminary interpretations of the derived Hapke parameters suggest that (1) red rocks can be modeled as a mixture of gray rocks with a coating of bright and dark soil or dust, and (2) gray rocks have macroscopically smoother surfaces composed of microscopically homogeneous, clear materials with little internal scattering, which may imply a glass-like or varnished surface. Copyright 1999 by the American Geophysical Union.

Acidalia and Chryse Plains, Mars

NASA Image and Video Library

2000-06-14

Somewhere down there sits the Mars Pathfinder lander and Sojourner rover. This Mars Global Surveyor Mars Orbiter Camera view of the red planet shows the region that includes Ares Vallis and the Chryse Plains upon which both Mars Pathfinder and the Viking 1 landed in 1997 and 1976, respectively. Acidalia Planitia is the dark surface that dominates the center left. The Pathfinder site is immediately south of Acidalia, just left of center in this view. Also shown--the north polar cap is at the top, and Arabia Terra and Sinus Meridiani are to the right. The bluish-white features are clouds. This is a color composite of 9 red and 9 blue image strips taken by the Mars Global Surveyor Mars Orbiter Camera on 9 successive orbits from pole-to-pole during the calibration phase of the mission in March 1999. The color is computer-enhanced and is not shown as it would actually appear to the human eye. http://photojournal.jpl.nasa.gov/catalog/PIA02000

Soil and Rock Yogi

NASA Image and Video Library

1997-07-06

Several possible targets of study for rover Sojourner's Alpha Proton X-Ray Spectrometer (APXS) instrument are seen in this image, taken by the Imager for Mars Pathfinder (IMP) on Sol 2. The smaller rock at left has been dubbed "Barnacle Bill," while the larger rock at right, approximately 3-4 meters from the lander, is now nicknamed "Yogi." Barnacle Bill is scheduled to be the first object of study for the APXS. Portions of a petal and deflated airbag are also visible at lower right. http://photojournal.jpl.nasa.gov/catalog/PIA00629

Sunset over "Twin Peaks"

NASA Image and Video Library

1997-08-06

This image was taken by the Imager for Mars Pathfinder (IMP) about one minute after sunset on Mars on Sol 21. The prominent hills dubbed "Twin Peaks" form a dark silhouette at the horizon, while the setting sun casts a pink glow over the darkening sky. The image was taken as part of a twilight study which indicates how the brightness of the sky fades with time after sunset. Scientists found that the sky stays bright for up to two hours after sunset, indicating that Martian dust extends very high into the atmosphere. http://photojournal.jpl.nasa.gov/catalog/PIA00783

Dust Wind Tails Around Rocks

NASA Technical Reports Server (NTRS)

1997-01-01

This image was taken by the Sojourner rover's left front camera on Sol 32. The Pathfinder lander is at right and is about 9 meters away. Wind tails of dust are clearly seen extending from the left side of many of the small rocks in the foreground. The large rocks on the horizon at left center are the next goal of Sojourner as it continues our exploration of Mars.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and managed the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

MOC's Highest Resolution View of Mars Pathfinder Landing Site

NASA Technical Reports Server (NTRS)

2000-01-01

[figure removed for brevity, see original site] (A) Mars Pathfinder site, left: April 1998; right: January 2000.

[figure removed for brevity, see original site] (B) top: April 1998; bottom: January 2000.

Can Mars Global Surveyor's 1.5 meter (5 ft) per pixel camera be used to find any evidence as to the fate of the Mars Polar Lander that was lost on December 3, 1999? One way to find out is to look for one of the other Mars landers and determine what, if anything, can be seen. There have been three successful Mars lander missions: Viking 1 (July 1976), Viking 2 (September 1976), and Mars Pathfinder (July 1997). Of these, the location of Mars Pathfinder is known the best because there are several distinct landmarks visible in the lander's images that help in locating the spacecraft. The MGS MOC Operations Team at Malin Space Science Systems has been tasked since mid-December 1999 with looking for the lost Polar Lander. Part of this effort has been to test the capabilities of MOC by taking a picture of the landing site of Mars Pathfinder.

An attempt to photograph the Pathfinder site was made once before, in April 1998, by turning the entire MGS spacecraft so that the camera could point at the known location of the Mars Pathfinder lander. Turning the MGS spacecraft like this is not a normal operation--it takes considerable planning, and disrupts the on-going, normal acquisition of science data. It took 3 attempts to succeed, but on April 22, 1998, MOC acquired the picture seen on the left side of Figure A, above. The three near-by major landmarks that were visible to the Pathfinder's cameras are labeled here (North Peak, Big Crater, Twin Peaks). It was known at the time that this image was not adequate to see the Pathfinder lander because the camera was not in focus and had a resolution of only 3.3 meters (11 ft) per pixel. In this and all other images shown here, north is up. All views of the 1998 MOC image are illuminated from the lower right, all views of the 2000 MOC image are illuminated from the lower left.

As part of the Polar Lander search effort, the Mars Pathfinder site was targeted again in December 1999 and January 2000. Like the 1998 attempt, the spacecraft had to be pointed off of its normal, nadir (straight-down) view. Like history repeating itself, it once again took 3 tries before the Pathfinder landing site was hit. The picture on the right side of Figure A, above, shows the new image that was acquired on January 16, 2000. The white box indicates the location shown in Figure B (above, right). The 1000 m scale bar equals 0.62 miles.

Figure B (above) shows a subsection of both the 1998 image (top, labeled SPO-1-25603) and the 2000 image (bottom, labeled m11-2414) projected at a scale of 3 meters (10 ft) per pixel. At this scale, the differences in camera focus and sunlight illumination angle are apparent, with the January 2000 image being both in focus and having better lighting conditions. In addition, the MGS spacecraft took the 2000 image from a lower altitude than in 1998, thus the image has better spatial resolution overall. The 500 m scale bar is equal to about 547 yards. The white box shows the location of images in Figure C, below.

[figure removed for brevity, see original site] (C) higher-resolution view; left: April 1998; right: January 2000.

[figure removed for brevity, see original site] D) Erroneous, preliminary identification of Mars Pathfinder location in January 2000 image. Subsequent analysis (Figures E & F, below) identified the correct spot.

The third figure (C, above) again shows portions of the April 1998 image (C, left) and January 2000 image (C, right), only this time they have been enlarged to a resolution of 0.75 meters (2.5 ft) per pixel. The intrinsic resolution of the January 2000 image is 1.5 meters (5 ft), so this is a 200% expanded view of the actual M11-02414 image. The circular features in this and the previous images are impact craters in various states of erosion. Some boulders (dark dots) can be seen near the crater in the lower left corner. The texture that runs diagonally across the scene from upper left toward lower right consists of ridges created by the giant floods that washed through the Pathfinder site from Ares and/or Tiu Vallis many hundreds of millions of years ago. These ridges and the troughs between them were also seen by the Pathfinder lander; their crests often covered with boulders and cobbles (which cannot be seen at the resolution of the MOC image). The 100 m scale bar is equal to 109 yards (which can be compared with a 100 yard U.S. football field). The Mars Pathfinder landing site is located near the center of this view.

The fourth picture, Figure D (above), shows a feature that was initially thought to be the Mars Pathfinder lander by MOC investigators. This and the following figures point out just how difficult it is to find a lander on the martian surface using the MGS MOC. Figure D was prepared early in the week following receipt of the new MOC image on January 17, 2000, and for several days it was believed that the lander had been found. As the subsequent two figures will show (E, and F, below), this location appears to be in error. How the features were misidentified is discussed below. Both Figure D and Figure F, showing possible locations of the Pathfinder lander in the MOC image, are enlarged by a factor of three over the intrinsic resolution of that image (that is, to a scale of 0.5 meters or about 1 ft, 7 inch per pixel). The right picture in Figure D shows sight-lines to the large horizon features--Big Crater, Twin Peaks, and North Peak--that were derived by the MOC team by looking at the images taken by the lander in 1997. After placing these lines on the overall image, there appeared to be two features close to the intersection of the sight-lines. Based upon the consistency of the size and shape of the lander as illuminated by sunlight in this image, the northern of the two candidate features (the small 'hump' at the center of both left and right pictures) was considered, at the time, to be the most likely. HOWEVER...

[figure removed for brevity, see original site] (E) Photoclinometry, Topography, and Revised Landing Site Location.

[figure removed for brevity, see original site] (F) Mars Pathfinder Landing Site; lander not resolved by MOC.

Later in the week following acquisition of the January 16, 2000, image (and over the following weekend), there was time for additional analysis to determine whether the rounded hump identified earlier in the week (Figure D, above) was, in fact, the Mars Pathfinder lander. A computer program that estimates relative topography in a MOC image from knowledge of the illumination (called 'shape-from-shading' or photoclinometry) was run to determine which parts of the landing site image are depressions, which are hills, and which are flat surfaces. The picture at the left in Figure E (above) shows the photoclinometry results for the area around the Pathfinder lander. The picture at the center of Figure E shows the same photoclinometry results overlain by an inset of a topographic map of the Pathfinder landing site derived by the U.S. Geological Survey Astrogeology Branch (Flagstaff, Arizona) from photogrammetry (parallax measurements) using images from Pathfinder's own stereo camera. By matching the features seen by MOC with those seen by the Pathfinder (the large arrows are examples of the matching), the location of the lander was refined and is now indicated in the picture on the right side of Figure E. The large, rounded hump previously identified as Pathfinder in Figure D (above), is more likely a large boulder that was seen in Pathfinder's images and named 'Couch' by the Pathfinder science team in 1997.

Figure F is summary of the results of this effort to find Mars Pathfinder: it shows that while the landing site of Mars Pathfinder can be identified, the lander itself cannot be seen. It is too small to be resolved in an image where each pixel acquired by the MOC covers a square of 1.5 meters (5 feet) to a side, given the contrast conditions on Mars and the MOC's ability to discriminate contrast. At this scale, Pathfinder is not much larger than two pixels, and the same is true of the lost Polar Lander.

No evidence has been found in the January 2000 MOC image of the aft portion of Mars Pathfinder's aeroshell or its parachute, either. If the aeroshell is laying on its side, as interpreted from Mars Pathfinder's images, then it would be very difficult to see this from orbit. Because Pathfinder did not image the parachute, it is not known how it may be configured on the surface--it could be wrapped around the aeroshell or a boulder, for example.

This effort to photograph the Mars Pathfinder lander demonstrates that it is extremely difficult to find a lander on the surface of Mars using the Mars Orbiter Camera aboard the MGS spacecraft. This analysis suggests that it is not very likely that the December 1999 Polar Lander will be found by MOC.

Weathering of rock 'Ginger'

NASA Technical Reports Server (NTRS)

1997-01-01

One of the more unusual rocks at the site is Ginger, located southeast of the lander. Parts of it have the reddest color of any material in view, whereas its rounded lobes are gray and relatively unweathered. These color differences are brought out in the inset, enhanced at the upper right. In the false color image at the lower right, the shape of the visible-wavelength spectrum (related to the abundance of weathered ferric iron minerals) is indicated by the hue of the rocks. Blue indicates relatively unweathered rocks. Typical soils and drift, which are heavily weathered, are shown in green and flesh tones. The very red color in the creases in the rock surface correspond to a crust of ferric minerals. The origin of the rock is uncertain; the ferric crust may have grown underneath the rock, or it may cement pebbles together into a conglomerate. Ginger will be a target of future super-resolution studies to better constrain its origin.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator. JPL is an operating division of the California Institute of Technology (Caltech).

High-gain Antenna & Terrain

NASA Image and Video Library

1997-07-06

Areas of rocky Martian terrain are seen in this image, taken by the Imager for Mars Pathfinder (IMP) on Sol 2. Portions of a lander petal and deflated airbag are at lower left. The dark disk at center is the high-gain antenna, and the silver cylindrical objects at upper right are part of the antenna's mechanism. An area of relatively smooth terrain is seen at upper right, which may offer clues to how this area was formed, and may be a future target for Sojourner's studies. The black area at lower right and small strip at top center is missing data. http://photojournal.jpl.nasa.gov/catalog/PIA00625

Cloud-Aerosol LIDAR and Infrared Pathfinder Satellite Observation (CALIPSO) Spacecraft: Independent Technical Assessment

NASA Technical Reports Server (NTRS)

Gilbrech, Richard J.; McManamen, John P.; Wilson, Timmy R.; Robinson, Frank; Schoren, William R.

2004-01-01

CALIPSO is a joint science mission between the CNES, LaRC and GSFC. It was selected as an Earth System Science Pathfinder satellite mission in December 1998 to address the role of clouds and aerosols in the Earth's radiation budget. The spacecraft includes a NASA light detecting and ranging (LIDAR) instrument, a NASA wide-field camera and a CNES imaging infrared radiometer. The scope of this effort was a review of the Proteus propulsion bus design and an assessment of the potential for personnel exposure to hydrazine propellant.

Cloud-Aerosol LIDAR and Infrared Pathfinder Satellite Observation (CALIPSO) Spacecraft: Independent Technical Assessment

NASA Technical Reports Server (NTRS)

Gilbrech, Richard J.; McManamen, John P.; Wilson, Timmy R.; Robinson, Frank; Schoren, William R.

2005-01-01

CALIPSO is a joint science mission between the CNES, LaRC and GSFC. It was selected as an Earth System Science Pathfinder satellite mission in December 1998 to address the role of clouds and aerosols in the Earth's radiation budget. The spacecraft includes a NASA light detecting and ranging (LIDAR) instrument, a NASA wide-field camera and a CNES imaging infrared radiometer. The scope of this effort was a review of the Proteus propulsion bus design and an assessment of the potential for personnel exposure to hydrazine propellant.

Extreme Rock Distributions on Mars and Implications for Landing Safety

NASA Technical Reports Server (NTRS)

Golombek, M. P.

2001-01-01

Prior to the landing of Mars Pathfinder, the size-frequency distribution of rocks from the two Viking landing sites and Earth analog surfaces was used to derive a size-frequency model, for nomimal rock distributions on Mars. This work, coupled with extensive testing of the Pathfinder airbag landing system, allowed an estimate of what total rock abundances derived from thermal differencing techniques could be considered safe for landing. Predictions based on this model proved largely correct at predicting the size-frequency distribution of rocks at the Mars Pathfinder site and the fraction of potentially hazardous rocks. In this abstract, extreme rock distributions observed in Mars Orbiter Camera (MOC) images are compared with those observed at the three landing sites and model distributions as an additional constraint on potentially hazardous surfaces on Mars.

False Color Processing to Enhance Differences Around Yogi

NASA Technical Reports Server (NTRS)

1997-01-01

In this scene showing the rover deployed at rock Yogi, the colors have similarly been enhanced to bring out differences. The same three kinds of rocks are recognized as in the distance. Yogi (red arrow), one of the large rocks with a weathered coating, exhibits a fresh face to the northeast, resulting perhaps from eolian scouring or from fracturing off of pieces to expose a fresher surface. Barnacle Bill and Cradle (blue arrows) are typical of the unweathered smaller rocks. During its traverse to Yogi the rover stirred the soil and exposed material from several cm in depth. During one of the turns to deploy Sojourner's Alpha Proton X-Ray Spectrometer (inset and white arrow), the wheels dug particularly deeply and exposed white material. Spectra of this white material show it is virtually identical to Scooby Doo, and such white material may underlie much of the site. The lander's rear ramp, which Sojourner used to descend to the Martian surface, is at lower left.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and managed the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator. JPL is an operating division of the California Institute of Technology (Caltech).

Correlation of Rock Spectra with Quantitative Morphologic Indices: Evidence for a Single Rock Type at the Mars Pathfinder Landing Site

NASA Technical Reports Server (NTRS)

Yingst, R. A.; Biedermann, K. L.; Pierre, N. M.; Haldemann, A. F. C.; Johnson, J. R.

2005-01-01

The Mars Pathfinder (MPF) landing site was predicted to contain a broad sampling of rock types varying in mineralogical, physical, mechanical and geochemical characteristics. Although rocks have been divided into several spectral categories based on Imager for Mars Pathfinder (IMP) visible/near-infrared data, efforts in isolating and classifying spectral units among MPF rocks and soils have met with varying degrees of success, as many factors influencing spectral signatures cannot be quantified to a sufficient level to be removed. It has not been fully determined which spectral categories stem from intrinsic mineralogical differences between rocks or rock surfaces, and which result from factors such as physical or chemical weathering. This has made isolation of unique rock mineralogies difficult. Morphology, like composition, is a characteristic tied to the intrinsic properties and geologic and weathering history of rocks. Rock morphologies can be assessed quantitatively and compared with spectral data, to identify and classify rock types at the MPF landing site. They can also isolate actual rock spectra from spectral types that are surficial in origin, as compositions associated with mantling dust or chemical coatings would presumably not influence rock morphology during weathering events. We previously reported on an initial classification of rocks using the quantitative morphologic indices of size, roundness, sphericity and elongation. Here, we compare this database of rock characteristics with associated rock surface spectra to improve our ability to discriminate between spectra associated with rock types and those from other sources.

Rock Garden Mosaic

NASA Technical Reports Server (NTRS)

1997-01-01

This image mosaic of part of the 'Rock Garden' was taken by the Sojourner rover's left front camera on Sol 71 (September 14). The rock 'Shark' is at left center and 'Half Dome' is at right. Fine-scale textures on the rocks are clearly seen. Broken crust-like material is visible at bottom center.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

VR for Mars Pathfinder

NASA Technical Reports Server (NTRS)

Blackmon, Theodore

1998-01-01

Virtual reality (VR) technology has played an integral role for Mars Pathfinder mission, operations Using an automated machine vision algorithm, the 3d topography of the Martian surface was rapidly recovered fro -a the stereo images captured. by the Tender camera to produce photo-realistic 3d models, An advanced, interface was developed for visualization and interaction with. the virtual environment of the Pathfinder landing site for mission scientists at the Space Flight Operations Facility of the Jet Propulsion Laboratory. The VR aspect of the display allowed mission scientists to navigate on Mars in Bud while remaining here on Earth, thus improving their spatial awareness of the rock field that surrounds the lenders Measurements of positions, distances and angles could be easily extracted from the topographic models, providing valuable information for science analysis and mission. planning. Moreover, the VR map of Mars has also been used to assist with the archiving and planning of activities for the Sojourner rover.

Spectral heterogeneity on Phobos and Deimos: HiRISE observations and comparisons to Mars Pathfinder results

USGS Publications Warehouse

Thomas, N.; Stelter, R.; Ivanov, A.; Bridges, N.T.; Herkenhoff, K. E.; McEwen, A.S.

2011-01-01

The High-Resolution Imaging Science Experiment (HiRISE) onboard Mars Reconnaissance Orbiter (MRO) has been used to observe Phobos and Deimos at spatial scales of around 6 and 20 m/px, respectively. HiRISE (McEwen et al.; JGR, 112, CiteID E05S02, DOI: 10.1029/2005JE002605, 2007) has provided, for the first time, high-resolution colour images of the surfaces of the Martian moons. When processed, by the production of colour ratio images for example, the data show considerable small-scale heterogeneity, which might be attributable to fresh impacts exposing different materials otherwise largely hidden by a homogenous regolith. The bluer material that is draped over the south-eastern rim of the largest crater on Phobos, Stickney, has been perforated by an impact to reveal redder material and must therefore be relatively thin. A fresh impact with dark crater rays has been identified. Previously identified mass-wasting features in Stickney and Limtoc craters stand out strongly in colour. The interior deposits in Stickney appear more inhomogeneous than previously suspected. Several other local colour variations are also evident. Deimos is more uniform in colour but does show some small-scale inhomogeneity. The bright streamers (Thomas et al.; Icarus, 123, 536556,1996) are relatively blue. One crater to the south-west of Voltaire and its surroundings appear quite strongly reddened with respect to the rest of the surface. The reddening of the surroundings may be the result of ejecta from this impact. The spectral gradients at optical wavelengths observed for both Phobos and Deimos are quantitatively in good agreement with those found by unresolved photometric observations made by the Imager for Mars Pathfinder (IMP; Thomas et al.; JGR, 104, 90559068, 1999). The spectral gradients of the blue and red units on Phobos bracket the results from IMP. ?? 2010 Elsevier Ltd. All rights reserved.

Wide-Angle Polarimetric Camera for Korea Pathfinder Lunar Orbiter

NASA Astrophysics Data System (ADS)

Choi, Y. J.; Kim, S.; Kang, K. I.

2016-12-01

A polarimetry data contains valuable information about the lunar surface such as the grain size and porosity of the regolith. However, a polarimetry toward the Moon in its orbit has not been performed. We plan to perform the polarimetry in lunar orbit through Korea Pathfinder Lunar Orbiter (KPLO), which will be launched around 2018/2019 as the first Korean lunar mission. Wide-Angle Polarimetric Camera (PolCam) is selected as one of the onboard instrument for KPLO. The science objectives are ; (1) To obtain the polarization data of the whole lunar surface at wavelengths of 430nm and 650nm for phase angle range from 0° to 120° with a spatial resolution of 80 m. (2) To obtain the reflectance ratios at 320 nm and 430 nm for the whole lunar surface with a spatial resolution of 80m. We will summarize recent results of lunar surface from ground-based polarimetric observations and will briefly introduce the science rationals and operation concept of PolCam.

Pebbles, Cobbles, and Sockets

NASA Technical Reports Server (NTRS)

1997-01-01

This Rover image of 'Shark' (upper left center), 'Half Dome' (upper right), and a small rock (right foreground) reveal textures and structures not visible in lander camera images. These rocks are interpreted as conglomerates because their surfaces have rounded protrusions up to several centimeters in size. It is suggested that the protrusions are pebbles and granules.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Wind Tails Near Chimp

NASA Technical Reports Server (NTRS)

1997-01-01

This image of the rock 'Chimp' was taken by the Sojourner rover's right front camera on Sol 72 (September 15). Fine-scale texture on Chimp and other rocks is clearly visible. Wind tails, oriented from lower right to upper left, are seen next to small pebbles in the foreground. These were most likely produced by wind action.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Stereo Images of Wind Tails Near Chimp

NASA Technical Reports Server (NTRS)

1997-01-01

This stereo image pair of the rock 'Chimp' was taken by the Sojourner rover's front cameras on Sol 72 (September 15). Fine-scale texture on Chimp and other rocks is clearly visible. Wind tails, oriented from lower right to upper left, are seen next to small pebbles in the foreground. These were most likely produced by wind action.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Pathfinder Rover, Airbags, & Martian Terrain

NASA Image and Video Library

1997-07-05

This is one of the first pictures taken by the camera on the Mars Pathfinder lander shortly after its touchdown at 10:07 AM Pacific Daylight Time on July 4, 1997. The small rover, named Sojourner, is seen in the foreground in its position on a solar panel of the lander. The white material on either side of the rover is part of the deflated airbag system used to absorb the shock of the landing. Between the rover and the horizon is the rock-strewn martian surface. Two hills are seen in the right distance, profiled against the light brown sky. http://photojournal.jpl.nasa.gov/catalog/PIA00611

Geologic Measurements using Rover Images: Lessons from Pathfinder with Application to Mars 2001

NASA Technical Reports Server (NTRS)

Bridges, N. T.; Haldemann, A. F. C.; Herkenhoff, K. E.

1999-01-01

The Pathfinder Sojourner rover successfully acquired images that provided important and exciting information on the geology of Mars. This included the documentation of rock textures, barchan dunes, soil crusts, wind tails, and ventifacts. It is expected that the Marie Curie rover cameras will also successfully return important information on landing site geology. Critical to a proper analysis of these images will be a rigorous determination of rover location and orientation. Here, the methods that were used to compute rover position for Sojourner image analysis are reviewed. Based on this experience, specific recommendations are made that should improve this process on the '01 mission.

Mars Pathfinder Landing Site and Surroundings

NASA Technical Reports Server (NTRS)

2007-01-01

NASA's Mars Pathfinder landed on Mars on July 4, 1997, and continued operating until Sept. 27 of that year. The landing site is on an ancient flood plain of the Ares and Tiu outflow channels. The High Resolution Imaging Science Experiment (HiRISE) camera on NASA's Mars Reconnaissance Orbiter took an image on Dec. 21, 2006, that provides unprecedented detail of the geology of the region and hardware on the surface.

[figure removed for brevity, see original site] HiRISE Image This is the entire image. The crater at center bottom was unofficially named 'Big Crater' by the Pathfinder team. Its wall was visible from Pathfinder, located 3 kilometers (2 miles) to the north. The two bright features to the upper left of Big Crater are the 'Twin Peaks,' also observed by Pathfinder. The bright mound to the upper right of the Twin Peaks is 'North Knob,' seen in Pathfinder images as peaking over the horizon.

At this scale there is no obvious geologic evidence of an ancient flood. Rather, impact craters dominate the scene, attesting to an old surface. The age is probably on the order of 1.8 billion to 3.5 billion years, when the Ares and Tiu floods are estimated to have occurred. Wind-formed linear ripples and dunes are seen throughout and are concentrated within craters. Sets of polygonal ridges of enigmatic origin are seen east of the Pathfinder lander. Rocks are visible over the entire image, with heavy concentrations near fresh-looking craters. Most of them are probably blocks tossed outward by crater-forming impacts.

The complete image is centered at 19.1 degrees north latitude, 326.8 degrees east longitude. The range to the target site was 284.7 kilometers (177.9 miles). At this distance the image scale is 28.5 centimeters (11 inches) per pixel, so objects about 85 centimeters (33 inches) across are resolved. The image shown here has been map-projected to 25 centimeters (10 inches) per pixel. North is up. The image was taken at a local Mars time of 3:35 p.m., and the scene is illuminated from the west with a solar incidence angle of 52 degrees, thus the sun was about 38 degrees above the horizon. At a solar longitude of 154.0 degrees, the season on Mars is northern summer.

[figure removed for brevity, see original site] Landing Site Region This is a close-up of the area in the vicinity of the Pathfinder landing site. Major features are named. The white box outlines the area of the image, discussed next, where hardware is seen.

[figure removed for brevity, see original site] Hardware on the Surface This image shows the Pathfinder lander on the surface. Zooming in, one can discern the ramps, science deck, and portions of the airbags on the Pathfinder lander. (See next image for closer view.) The back shell and parachute are to the south, and four features that may be portions of the heat shield are identified. Two of these were visible from Pathfinder. At the time of that mission, the nearest object was provisionally identified as the back shell. However, analysis of the HiRISE image and reinterpretation of Pathfinder images, plus an improved understanding of how hardware looks on the Martian surface based on ground-level and orbital images of the Mars Exploration Rover landing sites, indicate that the glint is bright enough that it may be insulating material from inside the heat shield. The back shell and parachute were out of sight behind a ridge from Pathfinder's ground view. One of the three bright features, identified as heat shield debris, was also identified during the Pathfinder mission.

[figure removed for brevity, see original site] [figure removed for brevity, see original site] Annotated Version Unannotated Version Topographic Map of Landing Site Region Portions of the HiRISE image are overlaid onto color-coded topographic maps constructed by the U.S. Geological Survey from stereo images acquired by the Imager for Mars Pathfinder on the lander. The white feature at the center is Pathfinder lander. The scales on the x and y axes are in meters, with the lander as the zero point. The color code for elevation relative to the lander is different in the left and right images, and shown in meters underneath each image. The correspondence between the overhead view revealed by HiRISE and the positions of topographic features inferred almost a decade ago from Pathfinder's horizontal view of the landscape is striking. The close-up on the right complements panoramas taken by the lander's camera, including the accompanying composite version showing the Sojourner rover at various locations it reached during the mission.

[figure removed for brevity, see original site] Mars Pathfinder Gallery Panorama This version of the Gallery Panorama taken with the lander's Imager for Mars Pathfinder camera shows many of the locations where the mission's Sojourner rover ended a Martian day during the 12-week mission. (There was only one Sojourner. The image is a composite.) One annotation indicates the last known position of Sojourner, near the rock 'Chimp,' at the time of the final data transmission from the lander. The location labeled 'Sojourner?' has been tentatively identified as the current position of the rover based on comparison of the ground-level view with the Dec. 21, 2006, image from NASA's Mars Reconnaissance Orbiter. At the proposed current location of the rover, a feature can be discerned in the 2006 orbital image that is about the right size for Sojourner and wasn't present when the Gallery Panorama was taken. Some rocks and other features that can be identified in the orbiter's high-resolution view are labeled in this ground-level view.

[figure removed for brevity, see original site] Topographic Perspective of Landing Site Region) This is a perspective view based on the topographic map and artificial color derived from Pathfinder and other data. The vertical scale is exaggerated by a factor of three, compared with horizontal dimensions. The white feature at center is the Pathfinder lander. It appears flat because the topographic map derived from the Imager for Mars Pathfinder data did not include the spacecraft itself.

The Role of APEX as a Pathfinder for AtLAST

NASA Astrophysics Data System (ADS)

Wyrowski, Friedrich

2018-01-01

Now more than 12 years in operation, the Atacama Pathfinder Experiment (APEX) 12 m submillimeter telescope has significantly contributed to a wide variety of submillimeter astronomy science areas, ranging from the discoveries of new molecules to large and deep imaging of the submillimeter sky. While ALMA operation is in full swing, APEX is strengthening its role not only as pathfinder for studying large source samples and spatial scales to prepare detailed high angular resolution ALMA follow ups, but also as fast response instruments to complement new results from ALMA. Furthermore, APEX ensures southern hemisphere access for submillimeter projects complementing archival Herschel research as well as new SOFIA science. With new broadband and multipixel receivers as well as large cameras for wide-field continuum imaging, APEX will pave the way towards the science envisioned with ATLAST. In this contribution, the current status and ongoing upgrades of APEX will be discussed, with an emphasis on the importance of continuous cutting edge science and state-of-the-art instrumentation that will bridge the gap towards ATLAST.

Martian Terrain, Unfurled Rover Ramps & Deflated Airbags

NASA Image and Video Library

1997-07-05

The Imager for Mars Pathfinder (IMP) took this image of surrounding terrain in the mid-morning on Mars (2:30 PM Pacific Daylight Time) earlier today. Part of the small rover, Sojourner, is visible on the left side of the picture. The tan cylinder to the right of the rover is one of two rolled-up ramps by which the rover will descend to the ground. The white, billowy material in the center of the picture is part of the airbag system. Many rocks of different shapes and sizes are visible between the lander and the horizon. Two hills are visible on the horizon. The notch on the left side of the leftmost conical hill is an artifact of the processing of this picture. http://photojournal.jpl.nasa.gov/catalog/PIA00613

Optical properties of the Martian aerosols as derived from Imager for Mars Pathfinder midday sky brightness data

NASA Astrophysics Data System (ADS)

Shalygina, O. S.; Markiewicz, W. J.; Hviid, S. F.

2012-09-01

It is well known that the aerosol play a major role in the energy budget of the Martian atmosphere. The importance of the aerosols for the radiative loading of the atmosphere has hence, direct impact on the Martian present weather and its seasonal cycle as well as consequences for its long term climate. Very accurate models of the sky brightness are required to separate the atmospheric illumination from the spectrum of the Martian surface, and hence to understand the mineralogy of the surface rocks and soil. Such accurate models are only possible if the optical properties of the Martian aerosols are known. In this work we analyze the images of the brightness of the Martian sky at midday acquired from the surface of the Mars during the Mars Pathfinder mission. The Imager for Mars Pathfinder (IMP) obtained data in filters centered at 443.6, 481.0, 670.8, 896.1 and 965.3 nm. Useful data sets were returned on sols 27, 40, 56, 65, 68, 74 and 82. Although the coverage in scattering angles of this sequence is limited to about 100°, having the Sun near zenith minimizes multiple scattering. This property should help in accuracy of constraining the size distribution and material properties. The shape of the particles can be expected to be less well constrained, as scattering events at angles around 150° are only present through multiple scattering. Data from sol 56 (Figure 1) were fitted with multiple scattering radiative transfer calculations to extract the size distribution, optical properties, and shape of the aerosols suspended in the atmosphere [1].

Pathfinder operations

NASA Technical Reports Server (NTRS)

Wilcher, J.; Stelzried, C.; Finley, S.

1986-01-01

In 1981, the Inter-Agency Consultative Group (composed of European, Soviet, Japanese and American space agency representatives) conceived the idea of using the two Soviet Vega spacecraft as pathfinders for Giotto since they would arrive at Halley's Comet approximately one week before Giotto. The Vega trajectory data and the Halley camera angle data were combined to improve the comet orbit accuracy. This was used to improve the Giotto fly-by targeting. The DSN performed delta DOR (VLBI) and one-way Doppler measurements of the Vega spacecraft for orbit determination. Although the early part-up phase had many problems, the results during the critical November 30, 1985 to March 4, 1986 operational phase had an overall 95 percent success rate, with 59 successes out of 62 two-station passes.

Mosaic of Wedge, Shark, Half-Dome, Moe and Flat Top

NASA Technical Reports Server (NTRS)

1997-01-01

The front cameras aboard the rover Sojourner imaged several prominent rocks on Sol 44. The highly-textured rock at left is Wedge, and in the background from left to right are Shark, Half-Dome, and Moe. The rectangular rock at right is Flat Top, which earlier close-up images revealed to be made up of elongated pits, possibly made by vesicles from volcanic outgassing or etches caused by weathering.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Constraints on Martian Soil Composition as Inferred from Viking XRFS and Pathfinder APXS and IMP Data

NASA Technical Reports Server (NTRS)

Bridges, N. T.; Crisp, J. A.

2000-01-01

With the successful operation of the Alpha Proton X-Ray Spectrometer (APXS) during 1997's Mars Pathfinder (MPF) mission, geochemistry data are now available from three sites on Mars. APXS raw spectra for six soils and five rocks have been converted to compositional abundances. The Viking Lander X-Ray Fluorescence Spectrometer (XRFS) successfully measured elemental abundances of nine soils at Viking 1 and eight soils at Viking 2. Although the three landing sites are located in different parts of Mars, the soils exhibit broad similarities, with an iron-rich chemistry similar to that of palagonite. However, the Pathfinder sods show some significant differences from Viking soils, notably an enrichment in silica and depletion in sulfur. The XRFS samples consisted of near-surface and deep (up to 22 cm) soils acquired by a collector head at the cod of a retractable boom. It was possible to collect and analyze pebbles as large a 2 cm, but only sod, some in the form of consolidated clods, was sampled. In contrast, the APXS measured materials in situ. This resulted in MPF "rock" analyses that probably had a significant dust component and, as explored here, "soil" analyses that may have contained a rocky component We examine several possibilities to explain these differences and other attributes of the APXS and XRFS data sets: 1) The APXS soil measurements actually sampled a mixture of Viking-like soil and small bits of high-silica, low-sulfur rock, 2) The soils were derived from high-silica rocks mixed with a minor component of globally-homogenized dust; these soils are chemically distinct and have a separate geologic history from the Viking soils. 3) The weathering environment was different at the Pathfinder landing site compared to the Viking sites, and 4) Uncertainties in the XRFS and APXS measurements result in reported elemental abundances different than those that are actually present We show that none of the possibilities can be discounted, but that an MPF soil distinct in composition from Viking sods is best supported by the available data.

Mars Pathfinder and Mars Global Surveyor Outreach Compilation

NASA Astrophysics Data System (ADS)

1999-09-01

This videotape is a compilation of the best NASA JPL (Jet Propulsion Laboratory) videos of the Mars Pathfinder and Mars Global Surveyor missions. The mission is described using animation and narration as well as some actual footage of the entire sequence of mission events. Included within these animations are the spacecraft orbit insertion; descent to the Mars surface; deployment of the airbags and instruments; and exploration by Sojourner, the Mars rover. JPL activities at spacecraft control during significant mission events are also included at the end. The spacecraft cameras pan the surrounding Mars terrain and film Sojourner traversing the surface and inspecting rocks. A single, brief, processed image of the Cydonia region (Mars face) at an oblique angle from the Mars Global Surveyor is presented. A description of the Mars Pathfinder mission, instruments, landing and deployment process, Mars approach, spacecraft orbit insertion, rover operation are all described using computer animation. Actual color footage of Sojourner as well as a 360 deg pan of the Mars terrain surrounding the spacecraft is provided. Lower quality black and white photography depicting Sojourner traversing the Mars surface and inspecting Martian rocks also is included.

Mars Pathfinder and Mars Global Surveyor Outreach Compilation

NASA Technical Reports Server (NTRS)

1999-01-01

This videotape is a compilation of the best NASA JPL (Jet Propulsion Laboratory) videos of the Mars Pathfinder and Mars Global Surveyor missions. The mission is described using animation and narration as well as some actual footage of the entire sequence of mission events. Included within these animations are the spacecraft orbit insertion; descent to the Mars surface; deployment of the airbags and instruments; and exploration by Sojourner, the Mars rover. JPL activities at spacecraft control during significant mission events are also included at the end. The spacecraft cameras pan the surrounding Mars terrain and film Sojourner traversing the surface and inspecting rocks. A single, brief, processed image of the Cydonia region (Mars face) at an oblique angle from the Mars Global Surveyor is presented. A description of the Mars Pathfinder mission, instruments, landing and deployment process, Mars approach, spacecraft orbit insertion, rover operation are all described using computer animation. Actual color footage of Sojourner as well as a 360 deg pan of the Mars terrain surrounding the spacecraft is provided. Lower quality black and white photography depicting Sojourner traversing the Mars surface and inspecting Martian rocks also is included.

Topographic Map of Pathfinder Landing Site

NASA Technical Reports Server (NTRS)

1997-01-01

Topographic map of the landing site, to a distance of 60 meters from the lander in the LSC coordinate system. The lander is shown schematically in the center; 2.5 meter radius circle (black) centered on the camera was not mapped. Gentle relief [root mean square (rms) elevation variation 0.5 m; rms a directional slope 4O] and organization of topography into northwest and northeast-trending ridges about 20 meters apart are apparent. Roughly 30% of the illustrated area is hidden from the camera behind these ridges. Contours (0.2 m interval) and color coding of elevations were generated from a digital terrain model, which was interpolated by kriging from approximately 700 measured points. Angular and parallax point coordinates were measured manually on a large (5 m length) anaglyphic uncontrolled mosaic and used to calculate Cartesian (LSC) coordinates. Errors in azimuth on the order of 10 are therefore likely; elevation errors were minimized by referencing elevations to the local horizon. The uncertainty in range measurements increases quadratically with range. Given a measurement error of 1/2 pixel, the expected precision in range is 0.3 meter at 10 meter range, and 10 meters at 60 meter range. Repeated measurements were made, compared, and edited for consistency to improve the range precision. Systematic errors undoubtedly remain and will be corrected in future maps compiled digitally from geometrically controlled images. Cartographic processing by U.S. Geological Survey.

NOTE: original caption as published in Science Magazine

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Mars Pathfinder flight system integration and test.

NASA Astrophysics Data System (ADS)

Muirhead, B. K.

This paper describes the system integration and test experiences, problems and lessons learned during the assembly, test and launch operations (ATLO) phase of the Mars Pathfinder flight system scheduled to land on the surface of Mars on July 4, 1997. The Mars Pathfinder spacecraft consists of three spacecraft systems: cruise stage, entry vehicle and lander. The cruise stage carries the entry and lander vehicles to Mars and is jettisoned prior to entry. The entry vehicle, including aeroshell, parachute and deceleration rockets, protects the lander during the direct entry and reduces its velocity from 7.6 to 0 km/s in stages during the 5 min entry sequence. The lander's touchdown is softened by airbags which are retracted once stopped on the surface. The lander then uprights itself, opens up fully and begins surface operations including deploying its camera and rover. This paper overviews the system design and the results of the system integration and test activities, including the entry, descent and landing subsystem elements. System test experiences including science instruments, the microrover, Sojourner, and software are discussed. The final qualification of the entry, descent and landing subsystems during this period is also discussed.

Windblown Dunes and Ripples

NASA Technical Reports Server (NTRS)

2003-01-01

MGS MOC Release No. MOC2-411, 4 July 2003

July 4, 2003, is the 6th anniversary of the Mars Pathfinder landing. One of the elements carried to the red planet by Pathfinder was the Wind Sock Experiment. This project was designed to measure wind activity by taking pictures of three aluminum 'wind socks.' While the winds at the Mars Pathfinder site did not blow particularly strong during the course of that mission, dust storms seen from orbit and Earth-based telescopes attest to the fact that wind is a major force of change on the dry, desert surface of Mars today. This Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) narrow angle image shows dark sand dunes and lighter-toned ripples trapped among the mountainous central peak of an old impact crater in Terra Tyrrhena near 13.9oS, 246.7oW. The dune slip faces--the steepest slope on the larger dunes--indicate sand transport is from the top/upper left toward the bottom/lower right. North is toward the top/upper right; the picture is 3 km (1.9 mi) across. Sunlight illuminates the scene from the upper left. This picture was obtained in April 2003.

Dust devil vortices seen by the Mars Pathfinder camera

USGS Publications Warehouse

Metzger, S.M.; Carr, J.R.; Johnson, J. R.; Parker, T.J.; Lemmon, M.T.

1999-01-01

Discovery of dust devil vortices in Mars Pathfinder (MPF) images reveals a dust entrainment mechanism at work on Mars. Scattering of visible light by dust in the Martian atmosphere creates a pronounced haze, preventing conventional image processing from displaying dust plumes. Spectral differencing techniques have enhanced five localized dust plumes from the general haze in images acquired near midday, which we determine to be dust devils. Processing of 440 nm images highlights dust devils as distinct occultation features against the horizon. The dust devils are interpreted to be 14-79 m wide, 46-350 m tall, travel at 0.5-4.6 m/s, with dust loading of 7E-5 kg m-3, relative to the general haze of 9E-8 kg m-3, and total particulate transport of 2.2 - 700 kg. The vortices match predictions from terrestrial analog studies. Copyright 1999 by the American Geophysical Union.

Evaluation of Origin Ensemble algorithm for image reconstruction for pixelated solid-state detectors with large number of channels

NASA Astrophysics Data System (ADS)

Kolstein, M.; De Lorenzo, G.; Mikhaylova, E.; Chmeissani, M.; Ariño, G.; Calderón, Y.; Ozsahin, I.; Uzun, D.

2013-04-01

The Voxel Imaging PET (VIP) Pathfinder project intends to show the advantages of using pixelated solid-state technology for nuclear medicine applications. It proposes designs for Positron Emission Tomography (PET), Positron Emission Mammography (PEM) and Compton gamma camera detectors with a large number of signal channels (of the order of 106). For PET scanners, conventional algorithms like Filtered Back-Projection (FBP) and Ordered Subset Expectation Maximization (OSEM) are straightforward to use and give good results. However, FBP presents difficulties for detectors with limited angular coverage like PEM and Compton gamma cameras, whereas OSEM has an impractically large time and memory consumption for a Compton gamma camera with a large number of channels. In this article, the Origin Ensemble (OE) algorithm is evaluated as an alternative algorithm for image reconstruction. Monte Carlo simulations of the PET design are used to compare the performance of OE, FBP and OSEM in terms of the bias, variance and average mean squared error (MSE) image quality metrics. For the PEM and Compton camera designs, results obtained with OE are presented.

Solar array model corrections from Mars Pathfinder lander data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ewell, R.C.; Burger, D.R.

1997-12-31

The MESUR solar array power model initially assumed values for input variables. After landing early surface variables such as array tilt and azimuth or early environmental variables such as array temperature can be corrected. Correction of later environmental variables such as tau versus time, spectral shift, dust deposition, and UV darkening is dependent upon time, on-board science instruments, and ability to separate effects of variables. Engineering estimates had to be made for additional shadow losses and Voc sensor temperature corrections. Some variations had not been expected such as tau versus time of day, and spectral shift versus time of day.more » Additions needed to the model are thermal mass of lander petal and correction between Voc sensor and temperature sensor. Conclusions are: the model works well; good battery predictions are difficult; inclusion of Isc and Voc sensors was valuable; and the IMP and MAE science experiments greatly assisted the data analysis and model correction.« less

Using Engineering Cameras on Mars Landers and Rovers to Retrieve Atmospheric Dust Loading

NASA Astrophysics Data System (ADS)

Wolfe, C. A.; Lemmon, M. T.

2014-12-01

Dust in the Martian atmosphere influences energy deposition, dynamics, and the viability of solar powered exploration vehicles. The Viking, Pathfinder, Spirit, Opportunity, Phoenix, and Curiosity landers and rovers each included the ability to image the Sun with a science camera that included a neutral density filter. Direct images of the Sun provide the ability to measure extinction by dust and ice in the atmosphere. These observations have been used to characterize dust storms, to provide ground truth sites for orbiter-based global measurements of dust loading, and to help monitor solar panel performance. In the cost-constrained environment of Mars exploration, future missions may omit such cameras, as the solar-powered InSight mission has. We seek to provide a robust capability of determining atmospheric opacity from sky images taken with cameras that have not been designed for solar imaging, such as lander and rover engineering cameras. Operational use requires the ability to retrieve optical depth on a timescale useful to mission planning, and with an accuracy and precision sufficient to support both mission planning and validating orbital measurements. We will present a simulation-based assessment of imaging strategies and their error budgets, as well as a validation based on archival engineering camera data.

Alignment Test Results of the JWST Pathfinder Telescope Mirrors in the Cryogenic Environment

NASA Technical Reports Server (NTRS)

Whitman, Tony L.; Wells, Conrad; Hadaway, James; Knight, J. Scott; Lunt, Sharon

2016-01-01

After integration of the Optical Telescope Element (OTE) to the Integrated Science Instrument Module (ISIM) to become the OTIS, the James Webb Space Telescope OTIS is tested at NASAs Johnson Space Center (JSC) in the cryogenic vacuum Chamber A for alignment and optical performance. The alignment of the mirrors comprises a sequence of steps as follows: The mirrors are coarsely aligned using photogrammetry cameras with reflective targets attached to the sides of the mirrors. Then a multi-wavelength interferometer is aligned to the 18-segment primary mirror using cameras at the center of curvature to align reflected light from the segments and using fiducials at the edge of the primary mirror. Once the interferometer is aligned, the 18 primary mirror segments are then adjusted to optimize wavefront error of the aggregate mirror. This process phases the piston and tilt positions of all the mirror segments. An optical fiber placed at the Cassegrain focus of the telescope then emits light towards the secondary mirror to create a collimated beam emitting from the primary mirror. Portions of the collimated beam are retro-reflected from flat mirrors at the top of the chamber to pass through the telescope to the SI detector. The image on the detector is used for fine alignment of the secondary mirror and a check of the primary mirror alignment using many of the same analysis techniques used in the on-orbit alignment. The entire process was practiced and evaluated in 2015 at cryogenic temperature with the Pathfinder telescope.

Dust deposition on the Mars Exploration Rover Panoramic Camera (Pancam) calibration targets

USGS Publications Warehouse

Kinch, K.M.; Sohl-Dickstein, J.; Bell, J.F.; Johnson, J. R.; Goetz, W.; Landis, G.A.

2007-01-01

The Panoramic Camera (Pancam) on the Mars Exploration Rover mission has acquired in excess of 20,000 images of the Pancam calibration targets on the rovers. Analysis of this data set allows estimates of the rate of deposition and removal of aeolian dust on both rovers. During the first 150-170 sols there was gradual dust accumulation on the rovers but no evidence for dust removal. After that time there is ample evidence for both dust removal and dust deposition on both rover decks. We analyze data from early in both rover missions using a diffusive reflectance mixing model. Assuming a dust settling rate proportional to the atmospheric optical depth, we derive spectra of optically thick layers of airfall dust that are consistent with spectra from dusty regions on the Martian surface. Airfall dust reflectance at the Opportunity site appears greater than at the Spirit site, consistent with other observations. We estimate the optical depth of dust deposited on the Spirit calibration target by sol 150 to be 0.44 ?? 0.13. For Opportunity the value was 0.39 ?? 0.12. Assuming 80% pore space, we estimate that the dust layer grew at a rate of one grain diameter per ???100 sols on the Spirit calibration target. On Opportunity the rate was one grain diameter per ???125 sols. These numbers are consistent with dust deposition rates observed by Mars Pathfinder taking into account the lower atmospheric dust optical depth during the Mars Pathfinder mission. Copyright 2007 by the American Geophysical Union.

Alignment test results of the JWST Pathfinder Telescope mirrors in the cryogenic environment

NASA Astrophysics Data System (ADS)

Whitman, Tony L.; Wells, Conrad; Hadaway, James B.; Knight, J. Scott; Lunt, Sharon

2016-07-01

After integration of the Optical Telescope Element (OTE) to the Integrated Science Instrument Module (ISIM) to become the OTIS, the James Webb Space Telescope OTIS is tested at NASA's Johnson Space Center (JSC) in the cryogenic vacuum Chamber A for alignment and optical performance. The alignment of the mirrors comprises a sequence of steps as follows: The mirrors are coarsely aligned using photogrammetry cameras with reflective targets attached to the sides of the mirrors. Then a multi-wavelength interferometer is aligned to the 18-segment primary mirror using cameras at the center of curvature to align reflected light from the segments and using fiducials at the edge of the primary mirror. Once the interferometer is aligned, the 18 primary mirror segments are then adjusted to optimize wavefront error of the aggregate mirror. This process phases the piston and tilt positions of all the mirror segments. An optical fiber placed at the Cassegrain focus of the telescope then emits light towards the secondary mirror to create a collimated beam emitting from the primary mirror. Portions of the collimated beam are retro-reflected from flat mirrors at the top of the chamber to pass through the telescope to the Science Instrument (SI) detector. The image on the detector is used for fine alignment of the secondary mirror and a check of the primary mirror alignment using many of the same analysis techniques used in the on-orbit alignment. The entire process was practiced and evaluated in 2015 at cryogenic temperature with the Pathfinder telescope.

Identification, Characterization, and Quantification of Impurities of Safinamide Mesilate: Process-Related Impurities and Degradation Products.

PubMed

Zou, Liang; Sun, Lili; Zhang, Hui; Hui, Wenkai; Zou, Qiaogen; Zhu, Zheying

2017-07-01

The characterization of process-related impurities and degradation products of safinamide mesilate (SAFM) in bulk drug and a stability-indicating HPLC method for the separation and quantification of all the impurities were investigated. Four process-related impurities (Imp-B, Imp-C, Imp-D, and Imp-E) were found in the SAFM bulk drug. Five degradation products (Imp-A, Imp-C, Imp-D, Imp-E, and Imp-F) were observed in SAFM under oxidative conditions. Imp-C, Imp-D, and Imp-E were also degradation products and process-related impurities. Remarkably, one new compound, identified as (S)-2-[4-(3-fluoro-benzyloxy) benzamido] propanamide (i.e., Imp-D), is being reported here as an impurity for the first time. Furthermore, the structures of the aforementioned impurities were characterized and confirmed via IR, NMR, and MS techniques, and the most probable formation mechanisms of all impurities proposed according to the synthesis route. Optimum separation was achieved on an Inertsil ODS-3 column (250 × 4.6 mm, 5 μm), using 0.1% formic acid in water (pH adjusted to 5.0) and acetonitrile as the mobile phase in gradient mode. The proposed method was found to be stability-indicating, precise, linear, accurate, sensitive, and robust for the quantitation of SAFM and its process-related substances, including its degradation products.

Oncofetal protein IMP3, a new cancer biomarker.

PubMed

Gong, Yuna; Woda, Bruce A; Jiang, Zhong

2014-05-01

IMP3 is a member of a family of RNA-binding proteins that consists of IMP1, IMP2 and IMP3. These proteins contain 2 RNA recognition motifs and 4 K-homology domains that allow them to bind RNAs strongly and specifically. IMP3 is an oncofetal protein involved in embryogenesis and its expression is associated with a number of malignant neoplasms. IMP3 is associated with aggressive and advanced cancers and is specifically expressed in malignant tumors but is not found in adjacent benign tissues. Moreover, in vitro studies have shown that IMP3 promotes tumor cell proliferation, adhesion, and invasion. This review focuses on the studies of IMP3 expression in different cancers and emphasizes the potential utility of IMP3 in routine surgical pathology practice. We also discuss IMP3 as a prognostic biomarker for cancer patients' outcomes.

IMP-29, a Novel IMP-Type Metallo-β-Lactamase in Pseudomonas aeruginosa

PubMed Central

Jeannot, Katy; Poirel, Laurent; Robert-Nicoud, Marjorie; Cholley, Pascal; Nordmann, Patrice

2012-01-01

Analysis of two clonally related multiresistant Pseudomonas aeruginosa isolates led to the identification of a novel IMP-type metallo-β-lactamase. IMP-29 was significantly different from the other IMP variants (the closest variant being IMP-5 with 93% amino acid identity). The blaIMP-29 gene cassette was carried by a class 1 integron in strain 10.298, while in strain 10.266 it was located in a rearranged DNA region on a 30-kb conjugative plasmid. Biochemical analysis confirmed that IMP-29 efficiently hydrolyzed carbapenems. PMID:22290960

IMP3 expression is associated with poor outcome and epigenetic deregulation in intrahepatic cholangiocarcinoma.

PubMed

Gao, Yuanyuan; Yang, Michelle; Jiang, Zhong; Woda, Bruce A; Mercurio, Arthur M; Qin, Jianjie; Huang, Xinli; Zhang, Feng

2014-06-01

IMP3 is a fetal protein not expressed in normal adult tissues. IMP3 is an oncoprotein and a useful biomarker for a variety of malignancies and is associated with reduced overall survival of a number of them. IMP3 expression and its prognostic value for patients with intrahepatic cholangiocarcinoma (ICC) have not been well investigated. The molecular mechanism underlying IMP3 expression in human cancer cells remains to be elucidated. Here we investigated IMP3 expression in ICC and adjacent nonneoplastic liver in 72 unifocal primary ICCs from a single institute by immunohistochemistry, immunoblotting, and real-time polymerase chain reaction. IMP3 was specifically expressed in cancer cells but not in the surrounding normal tissue, and 59 (82%) of 72 ICCs were IMP3 positive by immunohistochemistry. Among 35 cases with lymphovascular invasion, 26 (74%) showed IMP3 positivity in lymph node metastases. IMP3 expression was significantly correlated with tumor size, pathological grade, metastasis, and clinical stage. Kaplan-Meier analysis demonstrated an inverse correlation between IMP3 expression and overall survival rate. Multivariate analysis revealed that IMP3 was the only risk factor associated with survival. To further explore the mechanism of IMP3 expression in cancers, we identified 2 CpG islands at IMP3 proximal promoter. Interestingly, the IMP3 promoter was almost completely demethylated in ICCs in contrast to densely methylated promoter in normal liver tissues. IMP3 expression is a useful biomarker for ICCs and can provide an independent prognostic value for patients with ICC. To our knoweldge, this is the first direct evidence of epigenetic deregulation of IMP3 in human cancer. Copyright © 2014 The Auhtors. Published by Elsevier Inc. All rights reserved.

IGF2BP2/IMP2-Deficient mice resist obesity through enhanced translation of Ucp1 mRNA and Other mRNAs encoding mitochondrial proteins.

PubMed

Dai, Ning; Zhao, Liping; Wrighting, Diedra; Krämer, Dana; Majithia, Amit; Wang, Yanqun; Cracan, Valentin; Borges-Rivera, Diego; Mootha, Vamsi K; Nahrendorf, Matthias; Thorburn, David R; Minichiello, Liliana; Altshuler, David; Avruch, Joseph

2015-04-07

Although variants in the IGF2BP2/IMP2 gene confer risk for type 2 diabetes, IMP2, an RNA binding protein, is not known to regulate metabolism. Imp2(-/-) mice gain less lean mass after weaning and have increased lifespan. Imp2(-/-) mice are highly resistant to diet-induced obesity and fatty liver and display superior glucose tolerance and insulin sensitivity, increased energy expenditure, and better defense of core temperature on cold exposure. Imp2(-/-) brown fat and Imp2(-/-) brown adipocytes differentiated in vitro contain more UCP1 polypeptide than Imp2(+/+) despite similar levels of Ucp1 mRNA; the Imp2(-/-)adipocytes also exhibit greater uncoupled oxygen consumption. IMP2 binds the mRNAs encoding Ucp1 and other mitochondrial components, and most exhibit increased translational efficiency in the absence of IMP2. In vitro IMP2 inhibits translation of mRNAs bearing the Ucp1 untranslated segments. Thus IMP2 limits longevity and regulates nutrient and energy metabolism in the mouse by controlling the translation of its client mRNAs. Copyright © 2015 Elsevier Inc. All rights reserved.

KSC-05PD-1400

NASA Technical Reports Server (NTRS)

2005-01-01

KENNEDY SPACE CENTER, FLA. Inside the Astrotech Payload Processing Facility on Vandenberg Air Force Base in California, workers hold the Wide Field Camera that they will install on the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) spacecraft at right. CALIPSO will fly in combination with the CloudSat satellite to provide never-before-seen 3-D perspectives of how clouds and aerosols form, evolve, and affect weather and climate. CALIPSO and CloudSat will join three other satellites in orbit to enhance understanding of climate systems. The launch date for CALIPSO/CloudSat is no earlier than Aug. 22.

Global Molecular Epidemiology of IMP-Producing Enterobacteriaceae.

PubMed

Matsumura, Yasufumi; Peirano, Gisele; Motyl, Mary R; Adams, Mark D; Chen, Liang; Kreiswirth, Barry; DeVinney, Rebekah; Pitout, Johann D D

2017-04-01

International data on the molecular epidemiology of Enterobacteriaceae with IMP carbapenemases are lacking. We performed short-read (Illumina) whole-genome sequencing on a global collection of 38 IMP-producing clinical Enterobacteriaceae (2008 to 2014). IMP-producing Enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in the South Pacific and Asia. Specific bla IMP -containing integrons (In809 with bla IMP-4 , In722 with bla IMP-6 , and In687 with bla IMP-14 ) were circulating among different bacteria in countries such as Australia, Japan, and Thailand. In1312 with bla IMP-1 was present in Klebsiella pneumoniae from Japan and Citrobacter freundii from Brazil. Klebsiella pneumoniae ( n = 22) was the most common species; clonal complex 14 (CC14) from Philippines and Japan was the most common clone and contained In1310 with bla IMP-26 and In1321 with bla IMP-6 The Enterobacter cloacae complex ( n = 9) consisted of Enterobacter hormaechei and E. cloacae cluster III. CC78 (from Taiwan) containing In73 with bla IMP-8 was the most common clone among the E. cloacae complex. This study highlights the importance of surveillance programs using the latest molecular techniques for providing insight into the characteristics and global distribution of Enterobacteriaceae with bla IMP genes. Copyright © 2017 American Society for Microbiology.

Global Molecular Epidemiology of IMP-Producing Enterobacteriaceae

PubMed Central

Peirano, Gisele; Motyl, Mary R.; Adams, Mark D.; Chen, Liang; Kreiswirth, Barry; DeVinney, Rebekah

2017-01-01

ABSTRACT International data on the molecular epidemiology of Enterobacteriaceae with IMP carbapenemases are lacking. We performed short-read (Illumina) whole-genome sequencing on a global collection of 38 IMP-producing clinical Enterobacteriaceae (2008 to 2014). IMP-producing Enterobacteriaceae (7 varieties within 11 class 1 integrons) were mainly present in the South Pacific and Asia. Specific blaIMP-containing integrons (In809 with blaIMP-4, In722 with blaIMP-6, and In687 with blaIMP-14) were circulating among different bacteria in countries such as Australia, Japan, and Thailand. In1312 with blaIMP-1 was present in Klebsiella pneumoniae from Japan and Citrobacter freundii from Brazil. Klebsiella pneumoniae (n = 22) was the most common species; clonal complex 14 (CC14) from Philippines and Japan was the most common clone and contained In1310 with blaIMP-26 and In1321 with blaIMP-6. The Enterobacter cloacae complex (n = 9) consisted of Enterobacter hormaechei and E. cloacae cluster III. CC78 (from Taiwan) containing In73 with blaIMP-8 was the most common clone among the E. cloacae complex. This study highlights the importance of surveillance programs using the latest molecular techniques for providing insight into the characteristics and global distribution of Enterobacteriaceae with blaIMP genes. PMID:28167555

Energetic particle flux experiment (IMP-F and G)

NASA Technical Reports Server (NTRS)

Anderson, K. A.

1972-01-01

The technical aspects of the University of California IMP-F experiment aboard the Explorer-34 and the University of California IMP-G (S1) and (S2) experiments aboard the Explorer-41. The experiment detectors and electronics are discussed for each experiment as well as the fabrication, preflight and post-flight history. A description of the ground support equipment is also given for each experiment. These three experiments were essentially all different. The IMP-G experiment was essentially the IMP-F experiment with the addition of four Geiger-Mueller detectors. The IMP-G (S-2) was a supplementary experiment and differed completely from the IMP-F and IMP-G experiments. It was concluded that the ground support equipment approach used for the IMP-F and IMP-G experiments where emphasis was placed on a thorough exercise and monitoring of the experiment operation during various testing phases provided a high degree of confidence and reliability in these experiments. No known electronic failures have occurred during the spacecraft lifetime although some detector problems were experienced.

Molecular and epidemiological characterization of IMP-type metallo-β-lactamase-producing Enterobacter cloacae in a Large tertiary care hospital in Japan.

PubMed

Hayakawa, Kayoko; Miyoshi-Akiyama, Tohru; Kirikae, Teruo; Nagamatsu, Maki; Shimada, Kayo; Mezaki, Kazuhisa; Sugiki, Yuko; Kuroda, Emi; Kubota, Shiho; Takeshita, Nozomi; Kutsuna, Satoshi; Tojo, Masayoshi; Ohmagari, Norio

2014-06-01

IMP-type metallo-β-lactamase enzymes have been reported in different geographical areas and in various Gram-negative bacteria. However, the risk factors and epidemiology pertaining to IMP-type metallo-β-lactamase-producing Enterobacter cloacae (IMP-producing E. cloacae) have not been systematically evaluated. We conducted a retrospective, matched case-control study of patients from whom IMP-producing E. cloacae isolates were obtained, in addition to performing thorough molecular analyses of the clinically obtained IMP-producing E. cloacae isolates. Unique cases with IMP-producing E. cloacae isolation were included. Patients with IMP-producing E. cloacae were matched to uninfected controls at a ratio of 1 to 3. Fifteen IMP-producing E. cloacae cases were identified, with five of the isolates being obtained from blood, and they were matched to 45 uninfected controls. All (100%) patients from whom IMP-producing E. cloacae isolates were obtained had indwelling devices at the time of isolation, compared with one (2.2%) uninfected control. Independent predictors for isolation of IMP-producing E. cloacae were identified as cephalosporin exposure and invasive procedures within 3 months. Although in-hospital mortality rates were similar between cases and controls (14.3% versus 13.3%), the in-hospital mortality of patients with IMP-producing E. cloacae-caused bacteremia was significantly higher (40%) than the rate in controls. IMP-producing E. cloacae isolates were frequently positive for other resistance determinants. The MICs of meropenem and imipenem were not elevated; 10 (67%) and 12 (80%) of the 15 IMP-producing E. cloacae isolates had a MIC of ≤ 1 μg/ml. A phylogenetic tree showed a close relationship among the IMP-producing E. cloacae samples. Indwelling devices, exposure to cephalosporin, and a history of invasive procedures were associated with isolation of IMP-producing E. cloacae. Screening for carbapenemase production is important in order to apply appropriate clinical management and infection control measures. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Nuclear import of Nkx2-2 is mediated by multiple pathways

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Wenbo; Xu, PengPeng; Guo, YingYing

Nkx2-2 homeoprotein is essential for the development of the central nervous system and pancreas. Although the nuclear localization signals of Nkx2-2 have been identified, the responsible transport receptor is still unknown. Here, we demonstrate that imp α1 not only interacts with Nkx2-2 but also transports it into the nucleus in vitro by acting together with imp β1. However, the nuclear import of Nkx2-2 in cells was not inhibited in response to knockdown expression of endogenous imp β1 or over-expression of Bimax2. Furthermore, imp β1 and imp 13, but not imp 4, directly interact with Nkx2-2 and are capable of transporting Nkx2-2more » in an in vitro import assay. By GST pull-down assay, we demonstrate that mutation of NLS1 or NLS2 has no effect on interaction with imp α1 or imp 13, but significantly reduced binding to imp β1. Thus, the nuclear import of Nkx2-2 is mediated not only by the classical import pathway but also directly by imp β1 or imp 13.« less

Pathfinder-Plus takes off on flight in Hawaii

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on a flight over Hawaii in 1998. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on flight in Hawaii

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on a flight over Hawaii in 1998. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on a flight over Hawaiian island N'ihau

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on a flight over the Hawaiian island of N'ihau in 1998. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on flight over Hawaiian island N'ihau

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on a flight over the Hawaiian island of N'ihau in 1998. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on flight near Hawaiian island N'ihau

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on a flight with the Hawaiian island of N'ihau in the background. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on flight over Hawaii

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus flying over the Hawaiian Islands in 1998 with Ni'ihau Island in the background. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on flight over Hawaii

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on flight over Hawaii. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on a flight in Hawaii

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on a flight in 1998 over Hawaiian waters. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Pathfinder-Plus on flight over Hawaiian Islands

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on flight over Hawaiian Islands in 1998. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Intramyocardial pressure gradients in working and nonworking isolated cat hearts.

PubMed

Mihailescu, L S; Abel, F L

1994-03-01

This study presents an improved method for the measurement of intramyocardial pressure (IMP) using the servo-nulling mechanism. Glass micropipettes (20-24 microns OD) were used as transducers, coated to increase their mechanical resistance to breakage, and placed inside the left ventricular wall with a micropipette holder and manipulator. IMP was measured at the base of the left ventricle in working and nonworking isolated cat hearts that were perfused with Krebs-Henseleit buffer. In working hearts a transmural gradient of systolic IMP oriented from endocardium toward the epicardium was found; the endocardial values for systolic IMP were slightly higher than systolic left ventricular pressure (LVP), by 11-18%. Increases in afterload induced increases in IMP, without changing the systolic IMP-to-LVP ratio. In nonworking hearts with drained left ventricles, the systolic transmural gradient for IMP described for working hearts persisted, but at lower values, and was directly dependent on coronary perfusion pressure. Systolic IMP-to-LVP ratios were always > 1. The diastolic IMP of both working and nonworking hearts exhibited irregular transmural gradients. Our results support the view that generated systolic IMP is largely independent of LVP development.

PIA01554

NASA Image and Video Library

1999-07-02

This image of the rock "Flat Top" was taken from the left of the Sojourner rover's front cameras on Sol 42. Pits on the edge of the rock and a fluted surface are clearly visible. The rocks in the left background comprise the Rock Garden. Sojourner spent 83 days of a planned seven-day mission exploring the Martian terrain, acquiring images, and taking chemical, atmospheric and other measurements. The final data transmission received from Pathfinder was at 10:23 UTC on September 27, 1997. Although mission managers tried to restore full communications during the following five months, the successful mission was terminated on March 10, 1998. http://photojournal.jpl.nasa.gov/catalog/PIA01554

PIA01555

NASA Image and Video Library

1999-07-02

This image of the rock "Flat Top" was taken from the right of the Sojourner rover's front cameras on Sol 42. Pits on the edge of the rock and a fluted surface are clearly visible. The rocks in the left background comprise the "Rock Garden." Sojourner spent 83 days of a planned seven-day mission exploring the Martian terrain, acquiring images, and taking chemical, atmospheric and other measurements. The final data transmission received from Pathfinder was at 10:23 UTC on September 27, 1997. Although mission managers tried to restore full communications during the following five months, the successful mission was terminated on March 10, 1998. http://photojournal.jpl.nasa.gov/catalog/PIA01555

Insight into stereochemistry of a new IMP allelic variant (IMP-55) metallo-β-lactamase identified in a clinical strain of Acinetobacter baumannii.

PubMed

Shakibaie, Mohammad Reza; Azizi, Omid; Shahcheraghi, Fereshteh

2017-07-01

Metallo-β-lactamases (MBLs) such as IMPs are broad-spectrum β-lactamases that inactivate virtually all β-lactam antibiotics including carbapenems. In this study, we investigated the hydrolytic activity, phylogenetic relationship, three dimensional (3D) structure including zinc binding motif of a new IMP variant (IMP-55) identified in a clinical strain of Acinetobacter baumannii (AB). AB strain 56 was isolated from an adult ICU of a teaching hospital in Kerman, Iran. It exhibited MIC 32μg/ml to imipenem and showed MBL activity. Hydrolytic property of the MBL enzyme was measured phenotypically. Presence of bla IMP gene encoded by class 1 integrons was detected by PCR-sequencing. Phylogenetic tree of IMP protein was constructed using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and 3D model including zinc binding motif was predicted by bioinformatics softwares. Analysis of IMP sequence led to the identification of a novel IMP-type designated as IMP-55 (GenBank: KU299753.1; UniprotKB: A0A0S2MTX2). Impact in term of hydrolytic activity compared to the closest variants suggested efficient imipenem hydrolysis by this enzyme. Evolutionary distance matrix assessment indicated that IMP-55 protein is not closely related to other A. baumannii IMPs, however, shared 98% homology with Escherichia coli IMP-30 (UniprotKB: A0A0C5PJR0) and Pseudomonas aeruginosa IMP-1 (UniprotKB: Q19KT1). It consisted of five α-helices, ten β-sheets and six loops. A monovalent zinc ion attached to core of enzyme via His95, His97, His157 and Cys176. Multiple amino acid sequence alignments and mutational trajectory with reported IMPs showed 4 amino acid substitutions at positions 12(Phe→Ile), 31(Asp→Glu), 172(Leu→Phe) and 185(Asn→Lys). We suggest that the pleiotropic effect of mutations due to frequent administration of imipenem is responsible for emergence of new IMP variant in our hospitals. Copyright © 2017 Elsevier B.V. All rights reserved.

Description of IMP-31, a novel metallo-β-lactamase found in an ST235 Pseudomonas aeruginosa strain in Western Germany.

PubMed

Pfennigwerth, Niels; Geis, Gabriele; Gatermann, Sören G; Kaase, Martin

2015-07-01

The objective of this study was to characterize a novel IMP-type metallo-β-lactamase (MBL) found in an MDR clinical isolate of Pseudomonas aeruginosa. The P. aeruginosa isolate NRZ-00156 was recovered from an inguinal swab from a patient hospitalized in Western Germany and showed high MICs of carbapenems. MBL production was analysed by Etest for MBLs, an EDTA combined disc test and an EDTA bioassay. Typing of the isolate was performed by MLST. Genetic characterization of the new blaIMP gene was performed by sequencing the PCR products. A phylogenetic tree was constructed. The novel blaIMP gene was expressed in Escherichia coli TOP10 and the enzyme was subjected to biochemical characterization. The P. aeruginosa isolate NRZ-00156 expressed the ST235 allelic profile and was resistant to all the β-lactams tested except aztreonam. The isolate was positive for MBL production and harboured a new IMP allele, blaIMP-31, located on a disrupted class I integron [also carrying the blaOXA-35, aac(6')-Ib, aac(3)-Ic and aphA15 genes]. Its closest relative was IMP-35, with 96.7% amino acid identity. Expression of blaIMP-31 demonstrated that E. coli TOP10 producing IMP-31 had elevated resistance to all the β-lactams tested except aztreonam. Kinetic data were obtained for both IMP-31 and IMP-1. In comparison with IMP-1, IMP-31 showed weaker hydrolytic activity against all the β-lactams tested, which resulted from lower kcat values. The characterization of the new IMP-type gene blaIMP-31 from an ST235 P. aeruginosa isolate indicates an ongoing spread of highly divergent IMP-type carbapenemases in clinical P. aeruginosa strains and highlights the continuous need for the prevention of nosocomial infections caused by MDR Gram-negative bacteria. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Pathfinder-Plus on flight over Hawaiian Islands, with N'ihau and Lehua in the background

NASA Technical Reports Server (NTRS)

1998-01-01

Pathfinder-Plus on flight over Hawaiian Islands, with N'ihau and Lehua in the background. Pathfinder was a remotely controlled, solar-powered flying wing, designed and built as a proof-of-concept vehicle for a much larger aircraft capable of flying at extremely high altitudes for weeks at a time. It was built by AeroVironment, Inc., a California company that developed the human-powered Gossamer Condor and Gossamer Albatross lightweight aircraft during the 1970s, and later made the solar-electric powered Gossamer Penguin and Solar Challenger. The basic configuration and concepts for Pathfinder were first realized with the HALSOL (High Altitude Solar) aircraft, built in 1983 by AeroVironment and the Lawrence Livermore Laboratory. Pathfinder was constructed of advanced composites, plastics, and foam, and despite a wingspan of nearly 100 feet, it weighed only about 600 pounds. Pathfinder was one of several unpiloted prototypes under study by NASA's ERAST (Environmental Research Aircraft and Sensor Technology) program, a NASA-industry alliance which is helping develop advanced technologies that will enable aircraft to study the earth's environment during extremely long flights at altitudes in excess of 100,000 feet. (See project description below for Pathfinder's conversion to Pathfinder Plus.) In 1998, the Pathfinder solar-powered flying wing (see its photographs and project description) was modified into the longer-winged Pathfinder Plus configuration and on Aug. 6, 1998, Pathfinder Plus set an altitude record (for propeller-driven aircraft) of approximately 80,285 feet at the Pacific Missile Range Facility. The goal of the Pathfinder Plus flights was to validate new solar, aerodynamic, propulsion, and systems technology developed for its successor, the Centurion, which was designed to reach and sustain altitudes in the 100,000-foot range. The Centurion was succeeded by the Helios Prototype with a goal of reaching and sustaining flight at an altitude of 100,000 feet and flying non-stop for at least 4 days above 50,000 feet. Major activities of Pathfinder Plus' Hawaiian flights included detection of forest nutrient status, forest regrowth after damage caused by Hurricane Iniki in 1992, sediment/algal concentrations in coastal waters, and assessment of coral reef health. Pathfinder science activities were coordinated by NASA's Ames Research Center, Mountain View, California, and included researchers from the University of Hawaii and the University of California. Pathfinder is part of NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program managed by NASA's Dryden Flight Research Center, Edwards, California. Pathfinder and Pathfinder Plus were designed, built, and operated by AeroVironment, Inc., Monrovia, California. Pathfinder had a 98.4-foot wing span and weighed 560 pounds. Pathfinder Plus has a 121-foot wing span and weighs about 700 pounds. Pathfinder was powered by six electric motors while Pathfinder Plus has eight. Pathfinder's solar arrays produced approximately 8,000 watts of power while Pathfinder Plus' solar arrays produce about 12,500 watts of power. Both Pathfinder aircraft were built primarily of composites, plastic, and foam.

Evaluation of haloalkaliphilic sulfur-oxidizing microorganisms with potential application in the effluent treatment of the petroleum industry.

PubMed

Olguín-Lora, P; Le Borgne, S; Castorena-Cortés, G; Roldán-Carrillo, T; Zapata-Peñasco, I; Reyes-Avila, J; Alcántara-Pérez, S

2011-02-01

Haloalkaliphilic sulfur-oxidizing mixed cultures for the treatment of alkaline-saline effluents containing sulfide were characterized and evaluated. The mixed cultures (IMP-PB, IMP-XO and IMP-TL) were obtained from Mexican alkaline soils collected in Puebla (PB), Xochimilco (XO) and Tlahuac (TL), respectively. The Ribosomal Intergenic Spacer Analysis (RISA) revealed bacteria related to Thioalkalibacterium and Thioalkalivibrio in IMP-XO and IMP-PB mixed cultures. Halomonas strains were detected in IMP-XO and IMP-TL. In addition, an uncultured Bacteroides bacterium was present in IMP-TL. Mixed cultures were evaluated at different pH and NaCl concentrations at 30°C. IMP-PB and IMP-TL expressed thiosulfate-oxidizing activity in the 7.5-10.5 pH range, whereas IMP-XO presented its maximal activity with 19.0 mg O₂ g (protein)⁻¹ min⁻¹, at pH 10.6; it was not affected by NaCl concentrations up to 1.7 M. In continuous culture, IMP-XO showed a growth rate of 15 day⁻¹, productivity of 433.4 mg(protein) l⁻¹ day⁻¹ and haloalkaliphilic sulfur-oxidizing activity was also detected up to 170 mM by means of N-methyl-diethanolamine (MDEA). Saline-alkaline soil samples are potential sources of haloalkaliphilic sulfur-oxidizing bacteria and the mixed cultures could be applied in the treatment of inorganic sulfur compounds in petroleum industry effluents under alkaline-saline conditions.

Maps of the Martian Landing Sites and Rover Traverses: Viking 1 and 2, Mars Pathfinder, and Phoenix Landers, and the Mars Exploration Rovers.

NASA Astrophysics Data System (ADS)

Parker, T. J.; Calef, F. J., III; Deen, R. G.; Gengl, H.

2016-12-01

The traverse maps produced tactically for the MER and MSL rover missions are the first step in placing the observations made by each vehicle into a local and regional geologic context. For the MER, Phoenix and MSL missions, 25cm/pixel HiRISE data is available for accurately localizing the vehicles. Viking and Mars Pathfinder, however, relied on Viking Orbiter images of several tens of m/pixel to triangulate to horizon features visible both from the ground and from orbit. After Pathfinder, MGS MOC images became available for these landing sites, enabling much better correlations to horizon features and localization predictions to be made, that were then corroborated with HiRISE images beginning 9 years ago. By combining topography data from MGS, Mars Express, and stereo processing of MRO CTX and HiRISE images into orthomosaics (ORRs) and digital elevation models (DEMs), it is possible to localize all the landers and rover positions to an accuracy of a few tens of meters with respect to the Mars global control net, and to better than half a meter with respect to other features within a HiRISE orthomosaic. JPL's MIPL produces point clouds of the MER Navcam stereo images that can be processed into 1cm/pixel ORR/DEMs that are then georeferenced to a HiRISE/CTX base map and DEM. This allows compilation of seamless mosaics of the lander and rover camera-based ORR/DEMs with the HiRISE ORR/DEM that can be viewed in 3 dimensions with GIS programs with that capability. We are re-processing the Viking Lander, Mars Pathfinder, and Phoenix lander data to allow similar ORR/DEM products to be made for those missions. For the fixed landers and Spirit, we will compile merged surface/CTX/HiRISE ORR/DEMs, that will enable accurate local and regional mapping of these landing sites, and allow comparisons of the results from these missions to be made with current and future surface missions.

Intramuscular pressures for monitoring different tasks and muscle conditions

NASA Technical Reports Server (NTRS)

Sejersted, O. M.; Hargens, A. R.

1995-01-01

Intramuscular fluid pressure (IMP) can easily be measured in man and animals. It follows the law of Laplace which means that it is determined by the tension of the muscle fibers, the recording depth and by fiber geometry (fiber curvature or pennation angle). Thick, bulging muscles create high IMPs (up to 1000 mmHg) and force transmission to tendons becomes inefficient. High resting or postexercise IMPs are indicative of a compartment syndrome due to muscle swelling within a low-compliance osseofascial boundary. IMP increases linearly with force (torque) independent of the mode or speed of contraction (isometric, eccentric, concentric). IMP is also a much better predictor of muscle force than the EMG signal. During prolonged low-force isometric contractions, cyclic variations in IMP are seen. Since IMP influences muscle blood flow through the muscle pump, autoregulating vascular elements, and compression of the intramuscular vasculature, alterations in IMP have important implications for muscle function.

Effects of exogenous inosine monophosphate on growth performance, flavor compounds, enzyme activity, and gene expression of muscle tissues in chicken.

PubMed

Yan, Junshu; Liu, Peifeng; Xu, Liangmei; Huan, Hailin; Zhou, Weiren; Xu, Xiaoming; Shi, Zhendan

2018-04-01

The goal of this experiment was to examine effects of diets supplemented with exogenous inosine monophosphate (IMP) on the growth performance, flavor compounds, enzyme activity and gene expression of chicken. A total of 1,500 healthy, 1-day-old male 3-yellow chickens were used for a 52-d experimental period. Individuals were randomly divided into 5 groups (group I, II, III, IV, V) with 6 replicates per group, and fed a basal diet supplemented with 0.0, 0.05, 0.1, 0.2, and 0.3% IMP, respectively. There was no significant response to the increasing dietary IMP level in average daily feed intake (ADFI), average daily gain (ADG), and feed:gain ratio (F/G) (P ≥ 0.05). IMP content of the breast and thigh muscle showed an exponential and linear response to the increasing dietary IMP level (P < 0.05), the highest IMP content was obtained when the diet with 0.3% and 0.2% exogenous IMP was fed. There were significant effects of IMP level in diet on free amino acids (FAA) (exponential, linear and quadratic effect, P < 0.05) and delicious amino acids (DAA) (quadratic effect, P < 0.01) content in breast muscle. FAA and DAA content in thigh muscle showed an exponential and linear response (P < 0.05), and quadratic response (P < 0.01) to the increasing dietary IMP level, the highest FAA and DAA content was obtained when the diet with 0.2% exogenous IMP was fed. Dietary IMP supplementation had a quadratic effect on 5΄-NT and the alkaline phosphatase (ALP) enzyme activity in the breast muscle (P < 0.05), and the adenosine triphosphate (ATP) enzyme activity in the thigh muscles increased exponentially and linearly with increasing IMP level in diet (exponential effect, P = 0.061; linear effect, P = 0.059). Cyclohydrolase (ATIC) gene expression in thigh muscle had a quadratic response to the increasing dietary IMP level (P < 0.05), 0.2% exogenous IMP group had the highest (AMPD1) gene expression of the breast muscle and ATIC gene expression of the thigh muscle. These results indicate that dietary IMP did not affect the growth performance of chicken, the diet with 0.2 to 0.3% exogenous IMP is optimal to improve the meat flavor quality in chicken.

Leg intramuscular pressures during locomotion in humans

NASA Technical Reports Server (NTRS)

Ballard, R. E.; Watenpaugh, D. E.; Breit, G. A.; Murthy, G.; Holley, D. C.; Hargens, A. R.

1998-01-01

To assess the usefulness of intramuscular pressure (IMP) measurement for studying muscle function during gait, IMP was recorded in the soleus and tibialis anterior muscles of 10 volunteers during treadmill walking and running by using transducer-tipped catheters. Soleus IMP exhibited single peaks during late-stance phase of walking [181 +/- 69 (SE) mmHg] and running (269 +/- 95 mmHg). Tibialis anterior IMP showed a biphasic response, with the largest peak (90 +/- 15 mmHg during walking and 151 +/- 25 mmHg during running) occurring shortly after heel strike. IMP magnitude increased with gait speed in both muscles. Linear regression of soleus IMP against ankle joint torque obtained by a dynamometer produced linear relationships (n = 2, r = 0.97 for both). Application of these relationships to IMP data yielded estimated peak soleus moment contributions of 0.95-1.65 N . m/kg during walking, and 1.43-2.70 N . m/kg during running. Phasic elevations of IMP during exercise are probably generated by local muscle tissue deformations due to muscle force development. Thus profiles of IMP provide a direct, reproducible index of muscle function during locomotion in humans.

Retrieving Atmospheric Dust Loading on Mars Using Engineering Cameras and MSL's Mars Hand Lens Imager (MAHLI)

NASA Astrophysics Data System (ADS)

Wolfe, C. A.; Lemmon, M. T.

2015-12-01

Dust in the Martian atmosphere influences energy deposition, dynamics, and the viability of solar powered exploration vehicles. The Viking, Pathfinder, Spirit, Opportunity, Phoenix, and Curiosity landers and rovers each included the ability to image the Sun with a science camera equipped with a neutral density filter. Direct images of the Sun not only provide the ability to measure extinction by dust and ice in the atmosphere, but also provide a variety of constraints on the Martian dust and water cycles. These observations have been used to characterize dust storms, to provide ground truth sites for orbiter-based global measurements of dust loading, and to help monitor solar panel performance. In the cost-constrained environment of Mars exploration, future missions may omit such cameras, as the solar-powered InSight mission has. We seek to provide a robust capability of determining atmospheric opacity from sky images taken with cameras that have not been designed for solar imaging, such as the engineering cameras onboard Opportunity and the Mars Hand Lens Imager (MAHLI) on Curiosity. Our investigation focuses primarily on the accuracy of a method that determines optical depth values using scattering models that implement the ratio of sky radiance measurements at different elevation angles, but at the same scattering angle. Operational use requires the ability to retrieve optical depth on a timescale useful to mission planning, and with an accuracy and precision sufficient to support both mission planning and validating orbital measurements. We will present a simulation-based assessment of imaging strategies and their error budgets, as well as a validation based on the comparison of direct extinction measurements from archival Navcam, Hazcam, and MAHLI camera data.

Motility screen identifies Drosophila IGF-II mRNA-binding protein--zipcode-binding protein acting in oogenesis and synaptogenesis.

PubMed

Boylan, Kristin L M; Mische, Sarah; Li, Mingang; Marqués, Guillermo; Morin, Xavier; Chia, William; Hays, Thomas S

2008-02-01

The localization of specific mRNAs can establish local protein gradients that generate and control the development of cellular asymmetries. While all evidence underscores the importance of the cytoskeleton in the transport and localization of RNAs, we have limited knowledge of how these events are regulated. Using a visual screen for motile proteins in a collection of GFP protein trap lines, we identified the Drosophila IGF-II mRNA-binding protein (Imp), an ortholog of Xenopus Vg1 RNA binding protein and chicken zipcode-binding protein. In Drosophila, Imp is part of a large, RNase-sensitive complex that is enriched in two polarized cell types, the developing oocyte and the neuron. Using time-lapse confocal microscopy, we establish that both dynein and kinesin contribute to the transport of GFP-Imp particles, and that regulation of transport in egg chambers appears to differ from that in neurons. In Drosophila, loss-of-function Imp mutations are zygotic lethal, and mutants die late as pharate adults. Imp has a function in Drosophila oogenesis that is not essential, as well as functions that are essential during embryogenesis and later development. Germline clones of Imp mutations do not block maternal mRNA localization or oocyte development, but overexpression of a specific Imp isoform disrupts dorsal/ventral polarity. We report here that loss-of-function Imp mutations, as well as Imp overexpression, can alter synaptic terminal growth. Our data show that Imp is transported to the neuromuscular junction, where it may modulate the translation of mRNA targets. In oocytes, where Imp function is not essential, we implicate a specific Imp domain in the establishment of dorsoventral polarity.

IMP series report/bibliography

NASA Technical Reports Server (NTRS)

King, J. H.

1971-01-01

The main characteristics of the IMP spacecraft and experiments are considered and the scientific knowledge gained is presented in the form of abstracts of scientific papers using IMP data. Spacecraft characteristics, including temporal and spatial coverages, are presented followed by an annotated bibliography. Experiments conducted on all IMP's (including prelaunch IMP's H and J) are described. Figures are presented showing the time histories, through the end of 1970, of magnetic field, plasma, and energetic particle experiments.

The MVACS Surface Stereo Imager on Mars Polar Lander

NASA Astrophysics Data System (ADS)

Smith, P. H.; Reynolds, R.; Weinberg, J.; Friedman, T.; Lemmon, M. T.; Tanner, R.; Reid, R. J.; Marcialis, R. L.; Bos, B. J.; Oquest, C.; Keller, H. U.; Markiewicz, W. J.; Kramm, R.; Gliem, F.; Rueffer, P.

2001-08-01

The Surface Stereo Imager (SSI), a stereoscopic, multispectral camera on the Mars Polar Lander, is described in terms of its capabilities for studying the Martian polar environment. The camera's two eyes, separated by 15.0 cm, provide the camera with range-finding ability. Each eye illuminates half of a single CCD detector with a field of view of 13.8° high by 14.3° wide and has 12 selectable filters between 440 and 1000 nm. The f18 optics have a large depth of field, and no focusing mechanism is required; a mechanical shutter is avoided by using the frame transfer capability of the 528 × 512 CCD. The resolving power of the camera, 0.975 mrad/pixel, is the same as the Imager for Mars Pathfinder camera, of which it is nearly an exact copy. Specially designed targets are positioned on the Lander; they provide information on the magnetic properties of wind-blown dust, and radiometric standards for calibration. Several experiments beyond the requisite color panorama are described in detail: contour mapping of the local terrain, multispectral imaging of interesting features (possibly with ice or frost in shaded spots) to study local mineralogy, and atmospheric imaging to constrain the properties of the haze and clouds. Eight low-transmission filters are included for imaging the Sun directly at multiple wavelengths to give SSI the ability to measure dust opacity and potentially the water vapor content. This paper is intended to document the functionality and calibration of the SSI as flown on the failed lander.

MIG1-dependent and MIG1-independent regulation of GAL gene expression in Saccharomyces cerevisiae: role of Imp2p.

PubMed

Alberti, Adriana; Lodi, Tiziana; Ferrero, Iliana; Donnini, Claudia

2003-10-15

Imp2p (Yil154c) is a transcriptional activator involved in glucose derepression of the maltose, galactose and raffinose utilization pathways and in resistance to thermal, oxidative or osmotic stress. We analysed the role of Imp2 in the regulation of GAL genes. Imp2 was shown to have a positive effect on glucose derepression of Leloir pathway genes and their activator gene GAL4. The effect of Imp2 on galactose metabolism was shown to be partially dependent on Mig1p. The Mig1-independent role depends on Nrg1p. However, disruption of both MIG1 and NRG1 only partially relieves the glucose repression of GAL genes in the Deltaimp2 mutant, indicating that Imp2 must also have other function(s). Moreover, the interaction between IMP2 and GAL6/BLH1, a recently isolated gene involved in the regulation of GAL genes that shares with Imp2 the ability to protect cells from the glycopeptide bleomycin, was also analysed. The results suggest a major role of Imp2 in a GAL6-independent pathway. Copyright 2003 John Wiley & Sons, Ltd.

Structure of Importin-α from a Filamentous Fungus in Complex with a Classical Nuclear Localization Signal.

PubMed

Bernardes, Natalia E; Takeda, Agnes A S; Dreyer, Thiago R; Freitas, Fernanda Z; Bertolini, Maria Célia; Fontes, Marcos R M

2015-01-01

Neurospora crassa is a filamentous fungus that has been extensively studied as a model organism for eukaryotic biology, providing fundamental insights into cellular processes such as cell signaling, growth and differentiation. To advance in the study of this multicellular organism, an understanding of the specific mechanisms for protein transport into the cell nucleus is essential. Importin-α (Imp-α) is the receptor for cargo proteins that contain specific nuclear localization signals (NLSs) that play a key role in the classical nuclear import pathway. Structures of Imp-α from different organisms (yeast, rice, mouse, and human) have been determined, revealing that this receptor possesses a conserved structural scaffold. However, recent studies have demonstrated that the Impα mechanism of action may vary significantly for different organisms or for different isoforms from the same organism. Therefore, structural, functional, and biophysical characterization of different Impα proteins is necessary to understand the selectivity of nuclear transport. Here, we determined the first crystal structure of an Impα from a filamentous fungus which is also the highest resolution Impα structure already solved to date (1.75 Å). In addition, we performed calorimetric analysis to determine the affinity and thermodynamic parameters of the interaction between Imp-α and the classical SV40 NLS peptide. The comparison of these data with previous studies on Impα proteins led us to demonstrate that N. crassa Imp-α possess specific features that are distinct from mammalian Imp-α but exhibit important similarities to rice Imp-α, particularly at the minor NLS binding site.

[Expression of IMP3 in osteosarcoma and its clinical significance].

PubMed

Li, Kang-hua; Huang, Yue-ping; Zhang, Jun; Li, Guo-jun; Li, Si-hong

2009-05-01

To analyze the expressional variability of IMP3 between osteosarcoma and osteochondroma and explore its clinical significance. Paraffin sections from 68 patients with osteosarcoma and 20 patients with osteochondroma were examined for the expression of IMP3 by SP immunohistochemistry. The negative, weak positive, moderate positive and strong positive expression rates of IMP3 in 68 patients with osteosarcoma were 4.41% (3/68), 22.06% (15/68), 22.74% (19/68), 45.59% (31/68), respectively, which were significantly higher than those in 20 cases of osteochondroma tissues (P<0.01). The positive expression of IMP3 in osteosarcoma was negative correlation with the 3-year survival rate of osteosarcoma patients after the operation (P<0.01). The results suggest that IMP3 plays important roles in the tumorigenesis, progress and prognosis of osteosarcoma, and the expression of IMP3 may be an important feature of osteosarcoma.

Evaluating skeletal muscle electromechanical delay with intramuscular pressure.

PubMed

Go, Shanette A; Litchy, William J; Evertz, Loribeth Q; Kaufman, Kenton R

2018-06-08

Intramuscular pressure (IMP) is the fluid pressure generated within skeletal muscle and directly reflects individual muscle tension. The purpose of this study was to assess the development of force, IMP, and electromyography (EMG) in the tibialis anterior (TA) muscle during ramped isometric contractions and evaluate electromechanical delay (EMD). Force, EMG, and IMP were simultaneously measured during ramped isometric contractions in eight young, healthy human subjects. The EMD between the onset of force and EMG activity (Δt-EMG force) and the onset of IMP and EMG activity (Δt EMG-IMP) were calculated. A statistically significant difference (p < 0.05) was found between the mean force-EMG EMD (36 ± 31 ms) and the mean IMP-EMG EMD (3 ± 21 ms). IMP reflects changes in muscle tension due to the contractile muscle elements. Copyright © 2018 Elsevier Ltd. All rights reserved.

Experiences of intervertebral motion palpation in osteopathic practice - A qualitative interview study among Swedish osteopaths.

PubMed

Sposato, Niklas S; Bjerså, Kristofer

2017-01-01

Assessment in manual therapy includes quantitative and qualitative procedures, and intervertebral motion palpation (IMP) is one of the core assessment methods in osteopathic practice. The aim of this study was to explore osteopathic practitioners' experiences of clinical decision-making and IMP as a diagnostic tool for planning and evaluation of osteopathic interventions. The study was conducted with semi-structured interviews that included eight informants. Content analysis was used as the analytical procedure. In total, three categories emerged from the analysis: strategic decision-making, diagnostic usability of IMP, and treatment applicability of IMP. The study indicated that IMP was considered relevant and was given particular importance in cases where IMP findings confirmed clinical information attained from other stages in the diagnostic process as a whole. However, IMP findings were experienced as less important if they were not correlated to other findings. Copyright © 2016 Elsevier Ltd. All rights reserved.

IMP2, a nuclear gene controlling the mitochondrial dependence of galactose, maltose and raffinose utilization in Saccharomyces cerevisiae.

PubMed

Donnini, C; Lodi, T; Ferrero, I; Puglisi, P P

1992-02-01

The IMP2 gene of Saccharomyces cerevisiae is involved in the nucleo-mitochondrial control of maltose, galactose and raffinose utilization as shown by the inability of imp2 mutants to grow on these carbon sources in respiratory-deficient conditions or in the presence of ethidium bromide and erythromycin. The negative phenotype cannot be scored in the presence of inhibitors of respiration and oxidative phosphorylation, indicating that the role of the mitochondria in the utilization of the above-mentioned carbon sources in imp2 mutants is not at the energetical level. Mutations in the IMP2 gene also confer many phenotypic alterations in respiratory-sufficient conditions, e.g. leaky phenotype on oxidizable carbon sources, sensitivity to heat shock and sporulation deficiency. The IMP2 gene has been cloned, sequenced and disrupted. The phenotype of null imp2 mutants is indistinguishable from that of the originally isolated mutant.

Intramuscular Pressure Measurement During Locomotion in Humans

NASA Technical Reports Server (NTRS)

Ballard, Ricard E.

1996-01-01

To assess the usefulness of intramuscular pressure (IMP) measurement for studying muscle function during gait, IMP was recorded in the soleus and tibialis anterior muscles of ten volunteers during, treadmill walking, and running using transducer-tipped catheters. Soleus IMP exhibited single peaks during late-stance phase of walking (181 +/- 69 mmHg, mean +/- S.E.) and running (269 +/- 95 mmHg). Tibialis anterior IMP showed a biphasic response, with the largest peak (90 +/- 15 mmHg during walking and 151 +/- 25 mmHg during running) occurring shortly after heel strike. IMP magnitude increased with gait speed in both muscles. Linear regression of soleus IMP against ankle joint torque obtained by a dynamometer in two subjects produced linear relationships (r = 0.97). Application of these relationships to IMP data yielded estimated peak soleus moment contributions of 0.95-165 Nm/Kg during walking, and 1.43-2.70 Nm/Kg during running. IMP results from local muscle tissue deformations caused by muscle force development and thus, provides a direct, practical index of muscle function during locomotion in humans.

Management of Patients With Pancreatic Cysts: Analysis of Possible False-Negative Cases of Malignancy.

PubMed

Kowalski, Thomas; Siddiqui, Ali; Loren, David; Mertz, Howard R; Mallat, Damien; Haddad, Nadim; Malhotra, Nidhi; Sadowski, Brett; Lybik, Mark J; Patel, Sandeep N; Okoh, Emuejevoke; Rosenkranz, Laura; Karasik, Michael; Golioto, Michael; Linder, Jeffrey; Catalano, Marc F; Al-Haddad, Mohammad A

2016-09-01

To examine the utility of integrated molecular pathology (IMP) in managing surveillance of pancreatic cysts based on outcomes and analysis of false negatives (FNs) from a previously published cohort (n=492). In endoscopic ultrasound with fine-needle aspiration (EUS-FNA) of cyst fluid lacking malignant cytology, IMP demonstrated better risk stratification for malignancy at approximately 3 years' follow-up than International Consensus Guideline (Fukuoka) 2012 management recommendations in such cases. Patient outcomes and clinical features of Fukuoka and IMP FN cases were reviewed. Practical guidance for appropriate surveillance intervals and surgery decisions using IMP were derived from follow-up data, considering EUS-FNA sampling limitations and high-risk clinical circumstances observed. Surveillance intervals for patients based on IMP predictive value were compared with those of Fukuoka. Outcomes at follow-up for IMP low-risk diagnoses supported surveillance every 2 to 3 years, independent of cyst size, when EUS-FNA sampling limitations or high-risk clinical circumstances were absent. In 10 of 11 patients with FN IMP diagnoses (2% of cohort), EUS-FNA sampling limitations existed; Fukuoka identified high risk in 9 of 11 cases. In 4 of 6 FN cases by Fukuoka (1% of cohort), IMP identified high risk. Overall, 55% of cases had possible sampling limitations and 37% had high-risk clinical circumstances. Outcomes support more cautious management in such cases when using IMP. Adjunct use of IMP can provide evidence for relaxed surveillance of patients with benign cysts that meet Fukuoka criteria for closer observation or surgery. Although infrequent, FN results with IMP can be associated with EUS-FNA sampling limitations or high-risk clinical circumstances.

Oncofetal Protein IMP3: A Novel Molecular Marker That Predicts Metastasis of Papillary and Chromophobe Renal Cell Carcinomas

PubMed Central

Jiang, Zhong; Lohse, Christine M.; Chu, Peigou G.; Wu, Chin-Lee; Woda, Bruce A.; Rock, Kenneth L.; Kwon, Eugene D.

2009-01-01

BACKGROUND Whether an oncofetal protein, IMP3, can serve as a prognostic biomarker to predict metastasis for patients with localized papillary and chromophobe subtypes of renal cell carcinomas (RCCs) was investigated. METHODS The expression of IMP3 in 334 patients with primary papillary and chromophobe RCC from multiple medical centers was evaluated by immunohistochemistry. The 317 patients with localized papillary and chromophobe RCCs were further evaluated for outcome analyses. RESULTS IMP3 was significantly increased in a subset of localized papillary and chromophobe RCCs that subsequently metastasized. Patients with localized IMP3-positive tumors (n = 33; 10%) were over 10 times more likely to metastasize (risk ratio [RR], 11.38; 95% confidence interval [CI], 5.40–23.96; P <.001) and were nearly twice as likely to die (RR, 1.91; 95% CI, 1.13–3.22; P =.016) compared with patients with localized IMP3 negative tumors. The 5-year metastasis-free and overall survival rates were 64% and 58% for patients with IMP3-positive localized papillary and chromophobe RCCs compared with 98% and 85% for patients with IMP3 negative tumors, respectively. In multivariable analysis adjusting for the TNM stage and nuclear grade, patients with IMP3-positive tumors were still over 10 times more likely to progress to distant metastasis (RR, 13.45; 95% CI, 6.00–30.14; P <.001) and were still nearly twice as likely die (RR, 1.95; 95% CI, 1.15–3.31; P =.013) compared with patients with IMP3-negative tumors. CONCLUSIONS IMP3 is an independent prognostic biomarker that can be used to identify a subgroup of patients with localized papillary and chromophobe RCC who are at high risk for developing distant metastasis. PMID:18412154

Intramuscular Pressure of Tibialis Anterior Reflects Ankle Torque but Does Not Follow Joint Angle-Torque Relationship.

PubMed

Ateş, Filiz; Davies, Brenda L; Chopra, Swati; Coleman-Wood, Krista; Litchy, William J; Kaufman, Kenton R

2018-01-01

Intramuscular pressure (IMP) is the hydrostatic fluid pressure that is directly related to muscle force production. Electromechanical delay (EMD) provides a link between mechanical and electrophysiological quantities and IMP has potential to detect local electromechanical changes. The goal of this study was to assess the relationship of IMP with the mechanical and electrical characteristics of the tibialis anterior muscle (TA) activity at different ankle positions. We hypothesized that (1) the TA IMP and the surface EMG (sEMG) and fine-wire EMG (fwEMG) correlate to ankle joint torque, (2) the isometric force of TA increases at increased muscle lengths, which were imposed by a change in ankle angle and IMP follows the length-tension relationship characteristics, and (3) the electromechanical delay (EMD) is greater than the EMD of IMP during isometric contractions. Fourteen healthy adults [7 female; mean ( SD ) age = 26.9 (4.2) years old with 25.9 (5.5) kg/m 2 body mass index] performed (i) three isometric dorsiflexion (DF) maximum voluntary contraction (MVC) and (ii) three isometric DF ramp contractions from 0 to 80% MVC at rate of 15% MVC/second at DF, Neutral, and plantarflexion (PF) positions. Ankle torque, IMP, TA fwEMG, and TA sEMG were measured simultaneously. The IMP, fwEMG, and sEMG were significantly correlated to the ankle torque during ramp contractions at each ankle position tested. This suggests that IMP captures in vivo mechanical properties of active muscles. The ankle torque changed significantly at different ankle positions however, the IMP did not reflect the change. This is explained with the opposing effects of higher compartmental pressure at DF in contrast to the increased force at PF position. Additionally, the onset of IMP activity is found to be significantly earlier than the onset of force which indicates that IMP can be designed to detect muscular changes in the course of neuromuscular diseases impairing electromechanical transmission.

Incorporation of a recombinant Eimeria maxima IMP1 antigen into nanoparticles confers protective immunity against E. Maxima challenge infection.

PubMed

Jenkins, Mark C; Stevens, Laura; O'Brien, Celia; Parker, Carolyn; Miska, Katrzyna; Konjufca, Vjollca

2018-02-14

The purpose of this study was to determine if conjugating a recombinant Eimeria maxima protein, namely EmaxIMP1, into 20 nm polystyrene nanoparticles (NP) could improve the level of protective immunity against E. maxima challenge infection. Recombinant EmaxIMP1 was expressed in Escherichia coli as a poly-His fusion protein, purified by NiNTA chromatography, and conjugated to 20 nm polystyrene NP (NP-EmaxIMP1). NP-EMaxIMP1 or control non-recombinant (NP-NR) protein were delivered per os to newly-hatched broiler chicks with subsequent booster immunizations at 3 and 21 days of age. In battery cage studies (n = 4), chickens immunized with NP-EMaxIMP1 displayed complete protection as measured by weight gain (WG) against E. maxima challenge compared to chickens immunized with NP-NR. WG in the NP-EMaxIMP1-immunized groups was identical to WG in chickens that were not infected with E. maxima infected chickens. In floor pen studies (n = 2), chickens immunized with NP-EMaxIMP1 displayed partial protection as measured by WG against E. maxima challenge compared to chickens immunized with NP-NR. In order to understand the basis for immune stimulation, newly-hatched chicks were inoculated per os with NP-EMaxIMP1 or NP-NR protein, and the small intestine, bursa, and spleen, were examined for NP localization at 1 h and 6 h post-inoculation. Within 1 h, both NP-EMaxIMP1 and NP-NR were observed in all 3 tissues. An increase was observed in the level of NP-EmaxIMP1 and NP-NR in all tissues at 6 h post-inoculation. These data indicate that 20 nm NP-EmaxIMP1 or NP-NR reached deeper tissues within hours of oral inoculation and elicited complete to partial immunity against E. maxima challenge infection. Published by Elsevier Ltd.

Development and validation of a selective, sensitive and stability indicating UPLC-MS/MS method for rapid, simultaneous determination of six process related impurities in darunavir drug substance.

PubMed

A, Vijaya Bhaskar Reddy; Yusop, Zulkifli; Jaafar, Jafariah; Aris, Azmi B; Majid, Zaiton A; Umar, Khalid; Talib, Juhaizah

2016-09-05

In this study a sensitive and selective gradient reverse phase UPLC-MS/MS method was developed for the simultaneous determination of six process related impurities viz., Imp-I, Imp-II, Imp-III, Imp-IV, Imp-V and Imp-VI in darunavir. The chromatographic separation was performed on Acquity UPLC BEH C18 (50 mm×2.1mm, 1.7μm) column using gradient elution of acetonitrile-methanol (80:20, v/v) and 5.0mM ammonium acetate containing 0.01% formic acid at a flow rate of 0.4mL/min. Both negative and positive electrospray ionization (ESI) modes were operated simultaneously using multiple reaction monitoring (MRM) for the quantification of all six impurities in darunavir. The developed method was fully validated following ICH guidelines with respect to specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, robustness and sample solution stability. The method was able to quantitate Imp-I, Imp-IV, Imp-V at 0.3ppm and Imp-II, Imp-III, and Imp-VI at 0.2ppm with respect to 5.0mg/mL of darunavir. The calibration curves showed good linearity over the concentration range of LOQ to 250% for all six impurities. The correlation coefficient obtained was >0.9989 in all the cases. The accuracy of the method lies between 89.90% and 104.60% for all six impurities. Finally, the method has been successfully applied for three formulation batches of darunavir to determine the above mentioned impurities, however no impurity was found beyond the LOQ. This method is a good quality control tool for the trace level quantification of six process related impurities in darunavir during its synthesis. Copyright © 2016 Elsevier B.V. All rights reserved.

Imp2, the PSTPIP homolog in fission yeast, affects sensitivity to the immunosuppressant FK506 and membrane trafficking in fission yeast

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kita, Ayako; Higa, Mari; Doi, Akira

Cytokinesis is a highly ordered process that divides one cell into two cells, which is functionally linked to the dynamic remodeling of the plasma membrane coordinately with various events such as membrane trafficking. Calcineurin is a highly conserved serine/threonine protein phosphatase, which regulates multiple biological functions, such as membrane trafficking and cytokinesis. Here, we isolated imp2-c3, a mutant allele of the imp2{sup +} gene, encoding a homolog of the mouse PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1), using a genetic screen for mutations that are synthetically lethal with calcineurin deletion in fission yeast. The imp2-c3 mutants showed a defect in cytokinesis withmore » multi-septated phenotypes, which was further enhanced upon treatment with the calcineurin inhibitor FK506. Notably, electron micrographs revealed that the imp2-c3 mutant cells accumulated aberrant multi-lamella Golgi structures and putative post-Golgi secretory vesicles, and exhibited fragmented vacuoles in addition to thickened septa. Consistently, imp2-c3 mutants showed a reduced secretion of acid phosphatase and defects in vacuole fusion. The imp2-c3 mutant cells exhibited a weakened cell wall, similar to the membrane trafficking mutants identified in the same genetic screen such as ypt3-i5. These findings implicate the PSTPIP1 homolog Imp2 in Golgi/vacuole function, thereby affecting various cellular processes, including cytokinesis and cell integrity. - Highlights: • We isolated imp2-c3, in a synthetic lethal screen with calcineurin in fission yeast. • The imp2{sup +} gene encodes a component of the actin contractile ring similar to Cdc15. • The imp2-c3 mutants showed defects in cytokinesis, which were exacerbated by FK506. • The imp2-c3 mutants were defective in membrane trafficking and cell wall integrity. • Our study revealed a novel role for Imp2 in the Golgi/vacuolar membrane trafficking.« less

New Earth-Observing Instrument Installed on the International Space Station

NASA Image and Video Library

2017-12-08

In January 2013, a new Earth-observing instrument was installed on the International Space Station (ISS). ISERV Pathfinder consists of a commercial camera, a telescope, and a pointing system, all positioned to look through the Earth-facing window of ISS’s Destiny module. ISERV Pathfinder is intended as an engineering exercise, with the long-term goal of developing a system for providing imagery to developing nations as they monitor natural disasters and environmental concerns. The image above is the “first light” from the new ISERV camera system, taken at 1:44 p.m. local time on February 16, 2013. It shows the Rio San Pablo as it empties into the Golfo de Montijo in Veraguas, Panama. It is an ecological transition zone, changing from agriculture and pastures to mangrove forests, swamps, and estuary systems. The area has been designated a protected area by the National Environmental Authority (ANAM) of Panama and is listed as a “wetland of international importance” under the Ramsar Convention. (Note that the image is rotated so that north is to the upper right.) “ISERV’s full potential is yet to be seen, but we hope it will really make a difference in people’s lives,” said principal investigator Burgess Howell of NASA’s Marshall Space Flight Center. “For example, if an earthen dam gives way in Bhutan, we want to be able to show officials where the bridge is out or where a road is washed out or a power substation is inundated. This kind of information is critical to focus and speed rescue efforts.” The instrument will be controlled from NASA Marshall in Huntsville, Alabama, in collaboration with researchers at hubs in Central America, East Africa, and the Hindu Kush–Himalaya region. They will rely on positioning software to know where the space station is at each moment and to calculate the next chance to view a particular area on the ground. If there's a good viewing opportunity, the SERVIR team will instruct the camera to take high-resolution photographs at 3 to 7 frames per second, totaling as many as 100 images per pass. With a resolution down to 3.2 meters (10 feet), it will be possible to spot fairly small details and objects. The current mission will test the limitations of Pathfinder and identify measures for improvements in a more permanent system. For instance, the engineering team is working to determine how the geometry of the ISS window affects the imagery; how much sunlight is needed to capture clear images; and how the atmosphere affects that clarity. This characterization phase will last several weeks to a few months. Eventually, ISERV should be made available to the natural hazards community and to basic research scientists. ISERV is short for ISS SERVIR Environmental Research and Visualization system. Together with the U.S. Agency for International Development, NASA runs the SERVIR program to provide satellite data, maps, and other tools to environmental decisionmakers in developing countries. (Servir is a Spanish word meaning “to serve.”) Learn more about the SERVIR program by clicking here: servirglobal.net/Global.aspx NASA image by Burgess Howell, SERVIR Global program. Caption by Dauna Coulter, NASA Marshall Space Flight Center, and Mike Carlowicz, NASA Earth Observatory. Instrument: ISS - ISERV Pathfinder Credit: NASA Earth Observatory - 1.usa.gov/12Aqmg9 NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

40 CFR 86.1370-2007 - Not-To-Exceed test procedures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... regeneration event where regeneration is pending (state 1). (iii) If either N0 or N12 are zero, then RF cannot... absolute intake manifold pressure (IMP) for the corresponding IMP: ER06oc00.009 Where: P = absolute intake... absolute intake manifold pressure (IMP) for the corresponding IMP: ER06oc00.010 Where: P = absolute intake...

40 CFR 86.1370-2007 - Not-To-Exceed test procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... regeneration event where regeneration is pending (state 1). (iii) If either N0 or N12 are zero, then RF cannot... absolute intake manifold pressure (IMP) for the corresponding IMP: ER06oc00.009 Where: P = absolute intake... absolute intake manifold pressure (IMP) for the corresponding IMP: ER06oc00.010 Where: P = absolute intake...

40 CFR 86.1370 - Not-To-Exceed test procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... event where regeneration is pending (state 1). (iii) If either N0 or N12 are zero, then RF cannot be... absolute intake manifold pressure (IMP) for the corresponding IMP: ER06oc00.009 Where: P = absolute intake... absolute intake manifold pressure (IMP) for the corresponding IMP: ER06oc00.010 Where: P = absolute intake...

Effects of RNAi-Mediated Knockdown of Histone Methyltransferases on the Sex-Specific mRNA Expression of Imp in the Silkworm Bombyx mori

PubMed Central

Suzuki, Masataka G.; Ito, Haruka; Aoki, Fugaku

2014-01-01

Sexual differentiation in Bombyx mori is controlled by sex-specific splicing of Bmdsx, which results in the omission of exons 3 and 4 in a male-specific manner. In B. mori, insulin-like growth factor II mRNA-binding protein (Imp) is a male-specific factor involved in male-specific splicing of Bmdsx. Male-specific Imp mRNA results from the male-specific inclusion of exon 8. To verify the link between histone methylation and alternative RNA processing in Imp, we examined the effects of RNAi-mediated knockdown of several histone methyltransferases on the sex-specific mRNA expression of Imp. As a result, male-specific expression of Imp mRNA was completely abolished when expression of the H3K79 methyltransferase DOT1L was repressed to <10% of that in control males. Chromatin immunoprecipitation-quantitative PCR analysis revealed a higher distribution of H3K79me2 in normal males than in normal females across Imp. RNA polymerase II (RNAP II) processivity assays indicated that RNAi knockdown of DOT1L in males caused a twofold decrease in RNAP II processivity compared to that in control males, with almost equivalent levels to those observed in normal females. Inhibition of RNAP II-mediated elongation in male cells repressed the male-specific splicing of Imp. Our data suggest the possibility that H3K79me2 accumulation along Imp is associated with the male-specific alternative processing of Imp mRNA that results from increased RNAP II processivity. PMID:24758924

Immunophenotyping of the cerebrospinal fluid as a prognostic factor at diagnosis of acute lymphoblastic leukemia in children and adolescents.

PubMed

Cancela, Camila Silva Peres; Murao, Mitiko; Assumpção, Juliana Godoy; Souza, Marcelo Eduardo de Lima; de Macedo, Antonio Vaz; Viana, Marcos Borato; De Oliveira, Benigna Maria

2017-03-01

This study aimed at evaluating the use of immunophenotyping (IMP) in the identification of blast cells in the cerebrospinal fluid (CSF) of children and adolescents with acute lymphoblastic leukemia (ALL). Sixty-seven patients aged 18 years or younger were included. Fifty-five CSF samples were analyzed at initial diagnosis and 17 at the time of relapse. A cytological analysis (CA) was performed in all 72 samples, while IMP was done in 63. Blasts were identified in only three samples by CA, whereas all three samples were found negative by IMP, one of which had no isolation of nucleated cells after centrifugation. Among the samples analyzed by IMP, 11 showed a positive blast count, two of which had been inconclusive using CA. No equivalence was found between CA and IMP results (p = 0.55). CSF IMP positivity was not associated with other risk factors for ALL relapse. Among the 55 patients included at the time of diagnosis of ALL, eight relapsed during follow-up. Considering the cases of central nervous system (CNS) relapse, one of the patients belonged to the CSF IMP-positive group (11%) at diagnosis, and the other two cases, to the IMP-negative (5%) group. Detection of CSF blast cells using IMP was associated with a worse overall (p < 0.0001) and event-free survival (p < 0.0001). These results show that CSF IMP may be a useful additional method to conventional CA in the diagnosis of CNS involvement in ALL, and for the identification of high-risk subgroups that would benefit from an intensified therapy.

Dynamic Pathfinders: Leveraging Your OPAC to Create Resource Guides

ERIC Educational Resources Information Center

Hunter, Ben

2008-01-01

Library pathfinders are a time-tested method of leading library users to important resources. However, paper-based pathfinders suffer from space limitations, and both paper-based and Web-based pathfinders require frequent updates to keep up with new library acquisitions. This article details a step-by-step method to create an online dynamic…

The first 3':5'-cyclic nucleotide-amino acid complex: L-His-cIMP.

PubMed

Slepokura, Katarzyna

2012-08-01

In the crystal structure of the L-His-cIMP complex, i.e. L-histidinium inosine 3':5'-cyclic phosphate [systematic name: 5-(2-amino-2-carboxyethyl)-1H-imidazol-3-ium 7-hydroxy-2-oxo-6-(6-oxo-6,9-dihydro-1H-purin-9-yl)-4a,6,7,7a-tetrahydro-4H-1,3,5,2λ(5)-furo[3,2-d][1,3,2λ(5)]dioxaphosphinin-2-olate], C(6)H(10)N(3)O(2)(+)·C(10)H(10)N(4)O(7)P(-), the Hoogsteen edge of the hypoxanthine (Hyp) base of cIMP and the Hyp face are engaged in specific amino acid-nucleotide (His···cIMP) recognition, i.e. by abutting edge-to-edge and by π-π stacking, respectively. The Watson-Crick edge of Hyp and the cIMP phosphate group play a role in nonspecific His···cIMP contacts. The interactions between the cIMP anions (anti/C3'-endo/trans-gauche/chair conformers) are realized mainly between riboses and phosphate groups. The results for this L-His-cIMP complex, compared with those for the previously reported solvated L-His-IMP crystal structure, indicate a different nature of amino acid-nucleotide recognition and interactions upon the 3':5'-cyclization of the nucleotide phosphate group.

Differential expression of IMP3 between male and female mature teratomas--immunohistochemical evidence of malignant nature.

PubMed

Goodman, Steven; Zhang, Liping; Cheng, Liang; Jiang, Zhong

2014-10-01

Ovarian mature teratoma is a benign tumour, whereas mature teratoma in adult testicular germ cell tumours (TGCTs) is considered to be a malignant tumour. IMP3, an oncofetal protein, plays an important role in embryogenesis and carcinogenesis. IMP3 has been demonstrated to be a malignant biomarker that is mainly expressed in malignant neoplasms rather than benign tissues. The aim of this study was to analyse IMP3 expression in germ cell tumours, and compare its expression between male and female teratomas. One hundred and seventy-eight cases (62 TGCTs, 52 ovarian teratomas, 27 metastatic testicular teratomas, and 37 cases of normal testicular tissue) obtained from the archives of two large academic medical centres were examined for IMP3 expression. Of the 62 TGCTs, 30 had mature teratoma components. IMP3 expression was present in 100% (30/30) of testicular mature teratoma components, and in 96% (26/27) of metastatic testicular teratomas. Other TGCT components also expressed IMP3 in 99% of cases (78/79). IMP3 expression was negative in all female mature teratomas. We describe for the first time an immunostaining marker that has differential expression in male and female mature teratomas, indicating that their pathogenesis differs. High expression of IMP3 in adult mature testicular teratomas supports their malignant nature. © 2014 John Wiley & Sons Ltd.

Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner

PubMed Central

Lowe, Alan R; Tang, Jeffrey H; Yassif, Jaime; Graf, Michael; Huang, William YC; Groves, Jay T; Weis, Karsten; Liphardt, Jan T

2015-01-01

Soluble karyopherins of the importin-β (impβ) family use RanGTP to transport cargos directionally through the nuclear pore complex (NPC). Whether impβ or RanGTP regulate the permeability of the NPC itself has been unknown. In this study, we identify a stable pool of impβ at the NPC. A subpopulation of this pool is rapidly turned-over by RanGTP, likely at Nup153. Impβ, but not transportin-1 (TRN1), alters the pore's permeability in a Ran-dependent manner, suggesting that impβ is a functional component of the NPC. Upon reduction of Nup153 levels, inert cargos more readily equilibrate across the NPC yet active transport is impaired. When purified impβ or TRN1 are mixed with Nup153 in vitro, higher-order, multivalent complexes form. RanGTP dissolves the impβ•Nup153 complexes but not those of TRN1•Nup153. We propose that impβ and Nup153 interact at the NPC's nuclear face to form a Ran-regulated mesh that modulates NPC permeability. DOI: http://dx.doi.org/10.7554/eLife.04052.001 PMID:25748139

Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells.

PubMed

Yashiro, Ryu; Murota, Yukiko; Nishida, Kazumichi M; Yamashiro, Haruna; Fujii, Kaede; Ogai, Asuka; Yamanaka, Soichiro; Negishi, Lumi; Siomi, Haruhiko; Siomi, Mikiko C

2018-06-19

In Drosophila ovarian somatic cells (OSCs), Piwi represses transposons transcriptionally to maintain genome integrity. Piwi nuclear localization requires the N terminus and PIWI-interacting RNA (piRNA) loading of Piwi. However, the underlying mechanism remains unknown. Here, we show that Importinα (Impα) plays a pivotal role in Piwi nuclear localization and that Piwi has a bipartite nuclear localization signal (NLS). Impα2 and Impα3 are highly expressed in OSCs, whereas Impα1 is the least expressed. Loss of Impα2 or Impα3 forces Piwi to be cytoplasmic, which is rectified by overexpression of any Impα members. Extension of Piwi-NLS with an additional Piwi-NLS leads Piwi to be imported to the nucleus in a piRNA-independent manner, whereas replacement of Piwi-NLS with SV40-NLS fails. Limited proteolysis analysis suggests that piRNA loading onto Piwi triggers conformational change, exposing the N terminus to the environment. These results suggest that Piwi autoregulates its nuclear localization by exposing the NLS to Impα upon piRNA loading. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Intramuscular pressure and electromyography as indexes of force during isokinetic exercise

NASA Technical Reports Server (NTRS)

Aratow, M.; Ballard, R. E.; Grenshaw, A. G.; Styf, J.; Watenpaugh, D. E.; Kahan, N. J.; Hargens, A. R.

1993-01-01

A direct method for measuring force production of specific muscles during dynamic exercise is presently unavailable. Previous studies indicate that both intramuscular pressure (IMP) and electromyography (EMG) correlate linearly with muscle contraction force during isometric exercise. The objective of this study was to compare IMP and EMG as linear assessors of muscle contraction force during dynamic exercise. IMP and surface EMG activity were recorded during concentric and eccentric isokinetic plantarflexion and dorsiflexion of the ankle joint from the tibialis anterior (TA) and soleus (SOL) muscles of nine male volunteers. Ankle torque was measured using a dynamometer, and IMP was measured via catheterization. IMP exhibited better linear correlation than EMG with ankle joint torque during concentric contractions of the SOL and the TA, as well as during eccentric contractions. IMP provides a better index of muscle contraction force than EMG during concentric and eccentric exercise through the entire range of torque. IMP reflects intrinsic mechanical properties of individual muscles, such as length-tension relationships, which EMG is unable to assess.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaur, Gurpreet; Ly-Huynh, Jennifer D.; Jans, David A., E-mail: David.Jans@monash.edu

Highlights: • High intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import. • The effect of Ca{sup 2+} on nuclear import does not relate to changes in the nuclear pore. • High intracellular calcium can result in mislocalisation of Impβ1, Ran and RCC1. - Abstract: We previously showed that increased intracellular calcium can modulate Importin (Imp)β1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impβ1 andmore » Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.« less

Ras-sensitive IMP modulation of the Raf/MEK/ERK cascade through KSR1.

PubMed

Matheny, Sharon A; White, Michael A

2006-01-01

The E3 ubiquitin ligase IMP (impedes mitogenic signal propagation) was isolated as a novel Ras effector that negatively regulates ERK1/2 activation. Current evidence suggests that IMP limits the functional assembly of Raf/MEK complexes by inactivation of the KSR1 adaptor/scaffold protein. Interaction with Ras-GTP stimulates IMP autoubiquitination to relieve limitations on KSR function. The elevated sensitivity of IMP-depleted cells to ERK1/2 pathway activation suggests IMP acts as a signal threshold regulator by imposing reversible restrictions on the assembly of functional Raf/MEK/ERK kinase modules. These observations challenge commonly held concepts of signal transmission by Ras to the MAPK pathway and provide evidence for the role of amplitude modulation in tuning cellular responses to ERK1/2 pathway engagement. Here we describe details of the methods, including RNA interference, ubiquitin ligase assays, and protein complex analysis, that can be used to display the Ras-sensitive contribution of IMP to KSR-dependent modulation of the Raf/MEK/ERK pathway.

Pathfinder aircraft taking off - setting new solar powered altitude record

NASA Technical Reports Server (NTRS)

1995-01-01

The Pathfinder solar-powered remotely piloted aircraft climbs to a record-setting altitude of 50,567 feet during a flight Sept. 11, 1995, at NASA's Dryden Flight Research Center, Edwards, California. Pathfinder was a lightweight, solar-powered, remotely piloted flying wing aircraft used to demonstrate the use of solar power for long-duration, high-altitude flight. Its name denotes its mission as the 'Pathfinder' or first in a series of solar-powered aircraft that will be able to remain airborne for weeks or months on scientific sampling and imaging missions. Solar arrays covered most of the upper wing surface of the Pathfinder aircraft. These arrays provided up to 8,000 watts of power at high noon on a clear summer day. That power fed the aircraft's six electric motors as well as its avionics, communications, and other electrical systems. Pathfinder also had a backup battery system that could provide power for two to five hours, allowing for limited-duration flight after dark. Pathfinder flew at airspeeds of only 15 to 20 mph. Pitch control was maintained by using tiny elevators on the trailing edge of the wing while turns and yaw control were accomplished by slowing down or speeding up the motors on the outboard sections of the wing. On September 11, 1995, Pathfinder set a new altitude record for solar-powered aircraft of 50,567 feet above Edwards Air Force Base, California, on a 12-hour flight. On July 7, 1997, it set another, unofficial record of 71,500 feet at the Pacific Missile Range Facility, Kauai, Hawaii. In 1998, Pathfinder was modified into the longer-winged Pathfinder Plus configuration. (See the Pathfinder Plus photos and project description.)

Pathfinder aircraft taking off - setting new solar powered altitude record

NASA Technical Reports Server (NTRS)

1995-01-01

The Pathfinder solar-powered remotely piloted aircraft climbs to a record-setting altitude of 50,567 feet during a flight Sept. 11, 1995, at NASA's Dryden Flight Research Center, Edwards, California. The flight was part of the NASA ERAST (Environmental Research Aircraft and Sensor Technology) program. The Pathfinder was designed and built by AeroVironment Inc., Monrovia, California. Solar arrays cover nearly all of the upper wing surface and produce electricity to power the aircraft's six motors. Pathfinder was a lightweight, solar-powered, remotely piloted flying wing aircraft used to demonstrate the use of solar power for long-duration, high-altitude flight. Its name denotes its mission as the 'Pathfinder' or first in a series of solar-powered aircraft that will be able to remain airborne for weeks or months on scientific sampling and imaging missions. Solar arrays covered most of the upper wing surface of the Pathfinder aircraft. These arrays provided up to 8,000 watts of power at high noon on a clear summer day. That power fed the aircraft's six electric motors as well as its avionics, communications, and other electrical systems. Pathfinder also had a backup battery system that could provide power for two to five hours, allowing for limited-duration flight after dark. Pathfinder flew at airspeeds of only 15 to 20 mph. Pitch control was maintained by using tiny elevators on the trailing edge of the wing while turns and yaw control were accomplished by slowing down or speeding up the motors on the outboard sections of the wing. On September 11, 1995, Pathfinder set a new altitude record for solar-powered aircraft of 50,567 feet above Edwards Air Force Base, California, on a 12-hour flight. On July 7, 1997, it set another, unofficial record of 71,500 feet at the Pacific Missile Range Facility, Kauai, Hawaii. In 1998, Pathfinder was modified into the longer-winged Pathfinder Plus configuration. (See the Pathfinder Plus photos and project description.)

Increased IMP dehydrogenase gene expression in solid tumor tissues and tumor cell lines

DOE Office of Scientific and Technical Information (OSTI.GOV)

Collart, F.R.; Chubb, C.B.; Mirkin, B.L.

1992-07-10

IMP dehydrogenase, a regulatory enzyme of guanine nucleotide biosynthesis, may play a role in cell proliferation and malignancy. To assess this possibility, we examined IMP dehydrogenase expression in a series of human solid tumor tissues and tumor cell lines in comparison with their normal counterparts. Increased IMP dehydrogenase gene expression was observed in brain tumors relative to normal brain tissue and in sarcoma cells relative to normal fibroblasts. Similarly, in several B- and T-lymphoid leukemia cell lines, elevated levels of IMP dehydrogenase mRNA and cellular enzyme were observed in comparison with the levels in peripheral blood lymphocytes. These results aremore » consistent with an association between increased IMP dehydrogenase expression and either enhanced cell proliferation or malignant transformation.« less

Signaling threshold regulation by the Ras effector IMP.

PubMed

Matheny, Sharon A; White, Michael A

2009-04-24

The Ras effector and E3 ligase family member IMP (impedes mitogenic signal propagation) acts as a steady-state resistor within the Raf-MEK-ERK kinase module. IMP concentrations are directly regulated by Ras, through induction of autoubiquitination, to permit productive Raf-MEK complex assembly. Inhibition of Raf-MEK pathway activation by IMP occurs through the inactivation of KSR, a scaffold/adapter protein that couples activated Raf to its substrate MEK1. The capacity of IMP to inhibit signal propagation through Raf to MEK is, in part, a consequence of disrupting KSR1 homo-oligomerization and c-Raf-B-Raf hetero-oligomerization. These observations suggest that IMP functions as a threshold modulator, controlling sensitivity of the cascade to stimulus by directly limiting the assembly of functional KSR1-dependent Raf-MEK complexes.

Adaption of a corrector module to the IMP dynamics program

NASA Technical Reports Server (NTRS)

1972-01-01

The corrector module of the RAEIOS program and the IMP dynamics computer program were combined to achieve a date-fitting capability with the more general spacecraft dynamics models of the IMP program. The IMP dynamics program presents models of spacecraft dynamics for satellites with long, flexible booms. The properties of the corrector are discussed and a description is presented of the performance criteria and search logic for parameter estimation. A description is also given of the modifications made to add the corrector to the IMP program. This includes subroutine descriptions, common definitions, definition of input, and a description of output.

Effect of two non-synonymous ecto-5'-nucleotidase variants on the genetic architecture of inosine 5'-monophosphate (IMP) and its degradation products in Japanese Black beef.

PubMed

Uemoto, Yoshinobu; Ohtake, Tsuyoshi; Sasago, Nanae; Takeda, Masayuki; Abe, Tsuyoshi; Sakuma, Hironori; Kojima, Takatoshi; Sasaki, Shinji

2017-11-13

Umami is a Japanese term for the fifth basic taste and is an important sensory property of beef palatability. Inosine 5'-monophosphate (IMP) contributes to umami taste in beef. Thus, the overall change in concentration of IMP and its degradation products can potentially affect the beef palatability. In this study, we investigated the genetic architecture of IMP and its degradation products in Japanese Black beef. First, we performed genome-wide association study (GWAS), candidate gene analysis, and functional analysis to detect the causal variants that affect IMP, inosine, and hypoxanthine. Second, we evaluated the allele frequencies in the different breeds, the contribution of genetic variance, and the effect on other economical traits using the detected variants. A total of 574 Japanese Black cattle were genotyped using the Illumina BovineSNP50 BeadChip and were then used for GWAS. The results of GWAS showed that the genome-wide significant single nucleotide polymorphisms (SNPs) on BTA9 were detected for IMP, inosine, and hypoxanthine. The ecto-5'-nucleotidase (NT5E) gene, which encodes the enzyme NT5E for the extracellular degradation of IMP to inosine, was located near the significant region on BTA9. The results of candidate gene analysis and functional analysis showed that two non-synonymous SNPs (c.1318C > T and c.1475 T > A) in NT5E affected the amount of IMP and its degradation products in beef by regulating the enzymatic activity of NT5E. The Q haplotype showed a positive effect on IMP and a negative effect on the enzymatic activity of NT5E in IMP degradation. The two SNPs were under perfect linkage disequilibrium in five different breeds, and different haplotype frequencies were seen among breeds. The two SNPs contribute to about half of the total genetic variance in IMP, and the results of genetic relationship between IMP and its degradation products showed that NT5E affected the overall concentration balance of IMP and its degradation products. In addition, the SNPs in NT5E did not have an unfavorable effect on the other economical traits. Based on all the above findings taken together, two non-synonymous SNPs in NT5E would be useful for improving IMP and its degradation products by marker-assisted selection in Japanese Black cattle.

An Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and the TLR-5 agonist Salmonella typhimurium FliC flagellin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yin, Guangwen; Qin, Mei; Liu, Xianyong

Highlights: •We found a new protective protein – (IMPI) in Eimeria tenella. •EtIMP1-flagellin fusion protein is an effective immunogen against Eimeria infection. •Flagellin can be as an apicomplexan parasite vaccine adjuvant in chickens. -- Abstract: Immune mapped protein-1 (IMP1) is a new protective protein in apicomplexan parasites, and exits in Eimeria tenella. But its structure and immunogenicity in E. tenella are still unknown. In this study, IMPI in E. tenella was predicted to be a membrane protein. To evaluate immunogenicity of IMPI in E. tenella, a chimeric subunit vaccine consisting of E. tenella IMP1 (EtIMP1) and a molecular adjuvant (amore » truncated flagellin, FliC) was constructed and over-expressed in Escherichia coli and its efficacy against E. tenella infection was evaluated. Three-week-old AA broiler chickens were vaccinated with the recombinant EtIMP1-truncated FliC without adjuvant or EtIMP1 with Freund’s Complete Adjuvant. Immunization of chickens with the recombinant EtIMP1-truncated FliC fusion protein resulted in stronger cellular immune responses than immunization with only recombinant EtIMP1 with adjuvant. The clinical effect of the EtIMP1-truncated FliC without adjuvant was also greater than that of the EtIMP1 with adjuvant, which was evidenced by the differences between the two groups in body weight gain, oocyst output and caecal lesions of E. tenella-challenged chickens. The results suggested that the EtIMP1-flagellin fusion protein can be used as an effective immunogen in the development of subunit vaccines against Eimeria infection. This is the first demonstration of antigen-specific protective immunity against avian coccidiosis using a recombinant flagellin as an apicomplexan parasite vaccine adjuvant in chickens.« less

Activating PXR by Imperatorin Attenuates Dextran Sulphate Sodium-Induced Colitis in Mice.

PubMed

Liu, Meijing; Zhang, Guohui; Zheng, Chunge; Song, Meng; Liu, Fangle; Huang, Xiaotao; Bai, Shasha; Huang, Xinan; Lin, Chaozhan; Zhu, Chenchen; Hu, Yingjie; Mi, Suiqing; Liu, Changhui

2018-06-26

The activation of human pregnane X receptor (PXR) has potential therapeutic uses for inflammatory bowel disease (IBD). Imperatorin (IMP), a naturally-occurring coumarin, is the main bioactive ingredient of Angelica dahurica Radix, which is regularly used to treat the common cold and intestinal disorders. However, there are no data on the protective effects of IMP against IBD. The effects of IMP on PXR-modulated cytochrome P450 3A4 (CYP3A4) expression were assessed using a PXR transactivation assay, a mammalian two-hybrid assay, a competitive ligand-binding assay, analysis of CYP3A4 mRNA and protein expression levels, and measurement of CYP3A4 activity using a cell-based reporter gene assay and in vitro model. The inhibitory effects of IMP on NF-κB activity was evaluated by a reporter assay and NF-κB p65 nuclear translocation. The anti-IBD effects of IMP were investigated in a dextran sulphate sodium (DSS)-induced colitis mouse model. Colon inflammatory cytokines were assessed by ELISA. IMP activated CYP3A4 promoter activity, recruited steroid receptor coactivator 1 (SRC-1) to the ligand-binding domain of PXR, and increased the expression and activity of CYP3A4. However, PXR knockdown substantially reduced PXR-mediated CYP3A4 expression. Furthermore, IMP-mediated PXR activation suppressed NF-κB nuclear translocation and downregulated lipopolysaccharide-induced proinflammatory gene expression. Nevertheless, PXR knockdown partially reduced the IMP-mediated inhibition of NF-κB. IMP ameliorated DSS-induced colitis by PXR/NF-κB signalling. IMP serves as a PXR agonist to attenuate DSS-induced colitis by the suppression of the NF-κB-mediated proinflammatory response in a PXR/NF-κB- dependent manner. This article is protected by copyright. All rights reserved.

Impact of Internal Metallic Ports in Temporary Tissue Expanders on Postmastectomy Radiation Dose Distribution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Susie A.; Ogunleye, Tomiwa; Dhabbaan, Anees

Purpose: Temporary tissue expanders (TTE) with an internal magnetic metal port (IMP) have been increasingly used for breast reconstruction in post-mastectomy patients who receive radiation therapy (XRT). We evaluated XRT plans of patients with IMP to determine its effect on XRT dose distribution. Methods and Materials: Original treatment plans with CT simulation scans of 24 consecutive patients who received XRT (ORI), planned without heterogeneity corrections, to a reconstructed breast containing an IMP were used. Two additional treatment plans were then generated: one treatment plan with the IMP assigned the electron density of the rare earth magnet, nickel plated neodymium-iron-boron (HET),more » and a second treatment plan with the IMP assigned a CT value of 1 to simulate a homogeneous breast without an IMP (BRS). All plans were prescribed 50 Gy to the reconstructed breast (CTV). Results: CTV coverage by 50 Gy was significantly lower in the HET (mean 87.7% CTV) than in either the ORI (mean 99.7% CTV, P<.001) or BRS plans (mean 95.0% CTV, P<.001). The effect of the port was more pronounced on CT slices containing the IMP with prescription dose coverage of the CTV being less in the HET than in either ORI (mean difference 33.6%, P<.01) or BRS plans (mean difference 30.1%, P<.001). HET had a less homogeneous and conformal dose distribution than BRS or ORI. Conclusion: IMPs increase dose heterogeneity and reduce dose to the breast CTV through attenuation of the beam. For optimal XRT treatment, heterogeneity corrections should be used in XRT planning for patients with TTE with IMP, as the IMP impacts dose distribution.« less

Oligomerization of uridine phosphorimidazolides on montmorillonite: a model for the prebiotic synthesis of RNA on minerals

NASA Technical Reports Server (NTRS)

Ding, P. Z.; Kawamura, K.; Ferris, J. P.

1996-01-01

The 5'-phosphorimidazolide of uridine reacts on Na(+)-montmorillonite 22A in aqueous solution to give oligomers as long as 7 mers. The maximum chain length increases to 9 mers and the overall oligomer yield increases when 9:1 ImpU, A5' ppA mixtures react under the same conditions. The oligomer yield and maximum chain length decreases with the structure of the added pyrophosphate in the order A5' ppA > A5' ppU > U5' ppU. Structure analysis of individual oligomer fractions was performed by selective enzymatic hydrolyses followed by HPLC analysis of the products. The regioselectivity for 3',5'-bond formation is 80-90% in the 9:1 ImpU, A5' ppA reaction, a percentage comparable to that observed in the 9:1 ImpA, A5' ppA reaction. Oligomerization of ImpU is inhibited by addition of dA5' ppdA, and MeppA. No oligomers containing A5' ppU were products of the 9:1 ImpU,A5' ppA reaction, a finding consistent with the simple addition of the ImpU to the A5' ppA and not the rearrangement of an ImpU-A5' ppA adduct. Concentrations of lysine or arginine which were close to that of the ImpU did not inhibit oligomer formation. Treatment of Na(+)-montmorillonite with 1 M arginine yielded arginine-montmorillonite, an amino acid-mineral adduct which did not catalyze ImpU oligomerization. Neither the 4-9 mers formed in the 9:1 ImpU, A5' ppA reaction nor the 4-9 mers formed by the base hydrolysis of poly(U) served as templates for the formation of oligo(A)s.

An Experimental Design Approach for Impurity Profiling of Valacyclovir-Related Products by RP-HPLC

PubMed Central

Katakam, Prakash; Dey, Baishakhi; Hwisa, Nagiat T; Assaleh, Fathi H; Chandu, Babu R; Singla, Rajeev K; Mitra, Analava

2014-01-01

Abstract Impurity profiling has become an important phase of pharmaceutical research where both spectroscopic and chromatographic methods find applications. The analytical methodology needs to be very sensitive, specific, and precise which will separate and determine the impurity of interest at the 0.1% level. Current research reports a validated RP-HPLC method to detect and separate valacyclovir-related impurities (Imp-E and Imp-G) using the Box-Behnken design approach of response surface methodology. A gradient mobile phase (buffer: acetonitrile as mobile phase A and acetonitrile: methanol as mobile phase B) was used. Linearity was found in the concentration range of 50–150 μg/mL. The mean recovery of impurities was 99.9% and 103.2%, respectively. The %RSD for the peak areas of Imp-E and Imp-G were 0.9 and 0.1, respectively. No blank interferences at the retention times of the impurities suggest the specificity of the method. The LOD values were 0.0024 μg/mL for Imp-E and 0.04 μg/mL for Imp-G and the LOQ values were obtained as 0.0082 μg/mL and 0.136 μg/mL, respectively, for the impurities. The S/N ratios in both cases were within the specification limits. Proper peak shapes and satisfactory resolution with good retention times suggested the suitability of the method for impurity profiling of valacyclovir-related drug substances. PMID:25853072

Characteristics and management of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemase in a tertiary hospital.

PubMed

Pang, Feng; Jia, Xiu-Qin; Song, Zhen-Zhu; Li, Yan-Hua; Wang, Bin; Zhao, Qi-Gang; Wang, Chuan-Xin; Zhang, Yi; Wang, Le-Xin

2016-03-01

The emergence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases is rare. We report an occurrence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases in a Chinese tertiary care hospital from November 2010 to December 2012. The clinical characteristics of 30 patients were described. The genetic relationship of isolates was determined by pulsed-field gel electrophoresis (PFGE). Carbapenemases were detected by modified Hodge test (MHT) and polymerase chain reactions (PCRs). Amplicons were sequenced and blasted to determine the genotype. Most infected patients were from intensive care unit and had complex and serious underlying illnesses requiring mechanical ventilation. PFGE revealed that Klebsiella pneumoniae showed two major PFGE types. Two Klebsiella oxytoca had an indistinguishable PFGE pattern, while four Enterobacter cloacae were different strains. The sequencing studies showed Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemase in the 23 infected patients. The majority of patients had infections with the carbapenemase-producing Enterobacteriaceae (CPE) strain, most were successfully treated with a range of antibiotics and discharged. It is important to maintain a high index of suspicion to screen for carbapenemase-producing Enterobacteriaceae strains. Rapid identification of these strains and implementation of stringent procedures are the key to prevent major outbreaks in a hospital setting.

Hippo in Super Resolution from Super Panorama

NASA Image and Video Library

1998-07-03

This view of the "Hippo," 25 meters to the west of the lander, was produced by combining the "Super Panorama" frames from the IMP camera. Super resolution was applied to help to address questions about the texture of this rock and what it might tell us about its mode of origin. The composite color frames that make up this anaglyph were produced for both the right and left eye of the IMP. These composites consist of more than 15 frames per eye (because multiple sequences covered the same area), taken with different color filters that were enlarged by 500% and then co-added using Adobe Photoshop to produce, in effect, a super-resolution panchromatic frame that is sharper than an individual frame would be. These panchromatic frames were then colorized with the red, green, and blue filtered images from the same sequence. The color balance was adjusted to approximate the true color of Mars. The anaglyph view was produced by combining the left with the right eye color composite frames by assigning the left eye composite view to the red color plane and the right eye composite view to the green and blue color planes (cyan), to produce a stereo anaglyph mosaic. This mosaic can be viewed in 3-D on your computer monitor or in color print form by wearing red-blue 3-D glasses. http://photojournal.jpl.nasa.gov/catalog/PIA01421

Hyper-dependence of breast cancer cell types on the nuclear transporter Importin β1.

PubMed

Kuusisto, Henna V; Jans, David A

2015-08-01

We previously reported that overexpression of members of the Importin (Imp) superfamily of nuclear transporters results in increased nuclear trafficking through conventional transport pathways in tumour cells. Here we show for the first time that the extent of overexpression of Impβ1 correlates with disease state in the MCF10 human breast tumour progression system. Excitingly, we find that targeting Impβ1 activity through siRNA is >30 times more efficient in decreasing the viability of malignant ductal carcinoma cells compared to isogenic non-transformed counterparts, and is highly potent and tumour selective at subnanomolar concentrations. Tumour cell selectivity of the siRNA effects was unique to Impβ1 and not other Imps, with flow cytometric analysis showing >60% increased cell death compared to controls concomitant with reduced nuclear import efficiency as indicated by confocal microscopic analysis. This hypersensitivity of malignant cell types to Impβ1 knockdown raises the exciting possibility of anti-cancer therapies targeted at Impβ1. Copyright © 2015 Elsevier B.V. All rights reserved.

Imipenem Treatment Induces Expression of Important Genes and Phenotypes in a Resistant Acinetobacter baumannii Isolate.

PubMed

Dhabaan, Ghulam Nasser; AbuBakar, Sazaly; Cerqueira, Gustavo Maia; Al-Haroni, Mohammed; Pang, Sui Ping; Hassan, Hamimah

2015-12-14

Acinetobacter baumannii has emerged as a notorious multidrug-resistant pathogen, and development of novel control measures is of the utmost importance. Understanding the factors that play a role in drug resistance may contribute to the identification of novel therapeutic targets. Pili are essential for A. baumannii adherence to and biofilm formation on abiotic surfaces as well as virulence. In the present study, we found that biofilm formation was significantly induced in an imipenem-resistant (Imp(r)) strain treated with a subinhibitory concentration of antibiotic compared to that in an untreated control and an imipenem-susceptible (Imp(s)) isolate. Using microarray and quantitative PCR analyses, we observed that several genes responsible for the synthesis of type IV pili were significantly upregulated in the Imp(r) but not in the Imp(s) isolate. Notably, this finding is corroborated by an increase in the motility of the Imp(r) strain. Our results suggest that the ability to overproduce colonization factors in response to imipenem treatment confers biological advantage to A. baumannii and may contribute to clinical success. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

IMP3 Expression in Borderline Tumors of the Ovary.

PubMed

El-Balat, Ahmed; Sänger, Nicole; Karn, Thomas; Becker, Sven; Holtrich, Uwe; Muallem, Zelal; Arsenic, Ruza

2017-02-01

Borderline ovarian tumors (BOTs) have a less aggressive behavior than invasive epithelial ovarian tumors. Still some patients relapse or succumb to disease. Molecular markers that reliably predict prognosis are lacking. Insulin-like growth factor II mRNA-binding protein (IMP3) has been suggested as a prognostic marker in colorectal, hepatocellular, and ovarian clear-cell carcinomas. We analyzed the expression of IMP3 by immunohistochemistry in a cohort of 140 BOT and its association with histopathological features. We found no association of IMP3 expression with patients' age, FIGO stage, microinvasion, and presence of implants. In contrast, IMP3 expression correlated to mucinous subtype of BOTs (42.2% vs. 9.5% among other subtypes) (p<0.001). IMP3 expression was found to be associated with the presence of in situ carcinoma in MBOT, but not in other subtypes (p=0.021). Expression of IMP3 in BOT is associated with the mucinous subtype and may serve as an early indicator for the development of malignant features. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Mars Pathfinder Status at Launch

NASA Technical Reports Server (NTRS)

Spear, A. J.; Freeman, Delma C., Jr.; Braun, Robert D.

1996-01-01

The Mars Pathfinder Flight System is in final test, assembly and launch preparations at the Kennedy Space Center in Florida. Launch is scheduled for 2 Dec. 1996. The Flight System development, in particular the Entry, Descent, and Landing (EDL) system, was a major team effort involving JPL, other NASA centers and industry. This paper provides a summary Mars Pathfinder description and status at launch. In addition, a section by NASA's Langley Research Center, a key EDL contributor, is provided on their support to Mars Pathfinder. This section is included as an example of the work performed by Pathfinder team members outside JPL.

Pathfinder aircraft liftoff on altitude record setting flight of 71,500 feet

NASA Technical Reports Server (NTRS)

1997-01-01

The Pathfinder aircraft has set a new unofficial world record for high-altitude flight of over 71,500 feet for solar-powered aircraft at the U.S. Navy's Pacific Missile Range Facility, Kauai, Hawaii. Pathfinder was designed and manufactured by AeroVironment, Inc, of Simi Valley, California, and was operated by the firm under a jointly sponsored research agreement with NASA's Dryden Flight Research Center, Edwards, California. Pathfinder's record-breaking flight occurred July 7, 1997. The aircraft took off at 11:34 a.m. PDT, passed its previous record altitude of 67,350 feet at about 5:45 p.m. and then reached its new record altitude at 7 p.m. The mission ended with a perfect nighttime landing at 2:05 a.m. PDT July 8. The new record is the highest altitude ever attained by a propellor-driven aircraft. Before Pathfinder, the altitude record for propellor-driven aircraft was 67,028 feet, set by the experimental Boeing Condor remotely piloted aircraft. Pathfinder was a lightweight, solar-powered, remotely piloted flying wing aircraft used to demonstrate the use of solar power for long-duration, high-altitude flight. Its name denotes its mission as the 'Pathfinder' or first in a series of solar-powered aircraft that will be able to remain airborne for weeks or months on scientific sampling and imaging missions. Solar arrays covered most of the upper wing surface of the Pathfinder aircraft. These arrays provided up to 8,000 watts of power at high noon on a clear summer day. That power fed the aircraft's six electric motors as well as its avionics, communications, and other electrical systems. Pathfinder also had a backup battery system that could provide power for two to five hours, allowing for limited-duration flight after dark. Pathfinder flew at airspeeds of only 15 to 20 mph. Pitch control was maintained by using tiny elevators on the trailing edge of the wing while turns and yaw control were accomplished by slowing down or speeding up the motors on the outboard sections of the wing. On September 11, 1995, Pathfinder set a new altitude record for solar-powered aircraft of 50,567 feet above Edwards Air Force Base, California, on a 12-hour flight. On July 7, 1997, it set another, unofficial record of 71,500 feet at the Pacific Missile Range Facility, Kauai, Hawaii. In 1998, Pathfinder was modified into the longer-winged Pathfinder Plus configuration. (See the Pathfinder Plus photos and project description.)

Readout of the UFFO Slewing Mirror Telescope to detect UV/optical photons from Gamma-Ray Bursts

NASA Astrophysics Data System (ADS)

Kim, J. E.; Lim, H.; Nam, J. W.; Brandt, S.; Budtz-Jørgensen, C.; Castro-Tirado, A. J.; Chen, P.; Choi, H. S.; Grossan, B.; Huang, M. A.; Jeong, S.; Jung, A.; Kim, M. B.; Kim, S.-W.; Lee, J.; Linder, E. V.; Liu, T.-C.; Na, G. W.; Panasyuk, M. I.; Park, I. H.; Ripa, J.; Reglero, V.; Smoot, G. F.; Svertilov, S.; Vedenkin, N.; Yashin, I.

2013-07-01

The Slewing Mirror Telescope (SMT) was proposed for rapid response to prompt UV/optical photons from Gamma-Ray Bursts (GRBs). The SMT is a key component of the Ultra-Fast Flash Observatory (UFFO)-pathfinder, which will be launched aboard the Lomonosov spacecraft at the end of 2013. The SMT utilizes a motorized mirror that slews rapidly forward to its target within a second after triggering by an X-ray coded mask camera, which makes unnecessary a reorientation of the entire spacecraft. Subsequent measurement of the UV/optical is accomplished by a 10 cm aperture Ritchey-Chrètien telescope and the focal plane detector of Intensified Charge-Coupled Device (ICCD). The ICCD is sensitive to UV/optical photons of 200-650 nm in wavelength by using a UV-enhanced S20 photocathode and amplifies photoelectrons at a gain of 104-106 in double Micro-Channel Plates. These photons are read out by a Kodak KAI-0340 interline CCD sensor and a CCD Signal Processor with 10-bit Analog-to-Digital Converter. Various control clocks for CCD readout are implemented using a Field Programmable Gate Array (FPGA). The SMT readout is in charge of not only data acquisition, storage and transfer, but also control of the slewing mirror, the ICCD high voltage adjustments, power distribution, and system monitoring by interfacing to the UFFO-pathfinder. These functions are realized in the FPGA to minimize power consumption and to enhance processing time. The SMT readout electronics are designed and built to meet the spacecraft's constraints of power consumption, mass, and volume. The entire system is integrated with the SMT optics, as is the UFFO-pathfinder. The system has been tested and satisfies the conditions of launch and those of operation in space: those associated with shock and vibration and those associated with thermal and vacuum, respectively. In this paper, we present the SMT readout electronics: the design, construction, and performance, as well as the results of space environment test.

Mature DIABLO/Smac Is Produced by the IMP Protease Complex on the Mitochondrial Inner Membrane

PubMed Central

Burri, Lena; Strahm, Yvan; Hawkins, Christine J.; Gentle, Ian E.; Puryer, Michelle A.; Verhagen, Anne; Callus, Bernard; Vaux, David; Lithgow, Trevor

2005-01-01

DIABLO/Smac is a mitochondrial protein that can promote apoptosis by promoting the release and activation of caspases. To do so, DIABLO/Smac must first be processed by a mitochondrial protease and then released into the cytosol, and we show this in an intact cellular system. We propose that the precursor form of DIABLO/Smac enters the mitochondria through a stop-transfer pathway and is processed to its active form by the inner membrane peptidase (IMP) complex. Catalytic subunits of the mammalian IMP complex were identified based on sequence conservation and functional complementation, and the novel sequence motif RX5P in Imp1 and NX5S in Imp2 distinguish the two catalytic subunits. DIABLO/Smac is one of only a few specific proteins identified as substrates for the IMP complex in the mitochondrial intermembrane space. PMID:15814844

Pathfinder landing sites at candidate SNC impact ejection sites

NASA Technical Reports Server (NTRS)

Golombek, Matthew P.

1994-01-01

If Mars Pathfinder were able to land at a site on Mars from which the SNC meteorites were ejected by impact, the Pathfinder mission would essentially represent a very inexpensive sample return mission. Geologic units that contain four potential impact craters from which SNC meteorites could have been ejected from Mars are accessible to the Mars Pathfinder lander. Determining that SNC meteorites came from a particular spot on Mars raises the intriguing possibility of using Pathfinder as a sample return mission and providing a radiometric age for the considerably uncertain martian crater-age timescale. Pathfinder instruments are capable of determining if the rock type at the landing site is similar to that of one or more of the SNC meteorites, which would strengthen the hypothesis that the SNC meteorites did, in fact, come from Mars. Unfortunately, instrument observations from Pathfinder are probably not capable of determining if the geologic unit sampled by the lander is definitively the unit from which a SNC meteorite came from as opposed to Mars in general or perhaps a particular region on Mars. This abstract evaluates the possibility of landing at potential SNC ejection sites and the ability of Pathfinder to identify the landing site as the place from which an SNC meteorite came.

Piperacillin-tazobactam vs. imipenem-cilastatin as empirical therapy in hematopoietic stem cell transplantation recipients with febrile neutropenia.

PubMed

Jing, Yu; Li, Jian; Yuan, Lei; Zhao, Xiaoli; Wang, Quanshun; Yu, Li; Zhou, Daobin; Huang, Wenrong

2016-03-01

This randomized, dual-center study compared the efficacy and safety of piperacillin-tazobactam (PTZ) and imipenem-cilastatin (IMP) in hematopoietic stem cell transplantation (HSCT) recipients with febrile neutropenia. HSCT recipients with febrile neutropenia were randomized into two groups receiving either PTZ or IMP as initial empiric antibiotic. Endpoints were defervescence rate after empiric antibiotic for 48 h, success at end of therapy, and side effects. Defervescence within 48 h after empiric antibiotic was observed in 46 patients with PTZ (75.4%) and 59 patients with IMP (95.2%) (p = 0.002). Ten patients (10/46) in the PTZ group and two patients (2/59) in the IMP group switched empiric antibiotics due to recurrent fever (p = 0.005). Success of initial antibiotic with modification was achieved in 34 patients with PTZ (55.7%) and 53 patients with IMP (85.5%) at the end of therapy (p = 0.001). To treat the bacteremia, seven of 10 patients in the PTZ group and one of eight patients in the IMP group needed to switch the empiric antibiotic (p = 0.025). Compared with PTZ, IMP had more gastrointestinal adverse events (p = 0.045). This study demonstrates that IMP had better efficacy than PTZ as an empiric antibiotic for febrile neutropenia in the HSCT setting, but with more gastrointestinal side reactions. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Local requirement of the Drosophila insulin binding protein imp-L2 in coordinating developmental progression with nutritional conditions.

PubMed

Sarraf-Zadeh, Ladan; Christen, Stefan; Sauer, Uwe; Cognigni, Paola; Miguel-Aliaga, Irene; Stocker, Hugo; Köhler, Katja; Hafen, Ernst

2013-09-01

In Drosophila, growth takes place during the larval stages until the formation of the pupa. Starvation delays pupariation to allow prolonged feeding, ensuring that the animal reaches an appropriate size to form a fertile adult. Pupariation is induced by a peak of the steroid hormone ecdysone produced by the prothoracic gland (PG) after larvae have reached a certain body mass. Local downregulation of the insulin/insulin-like growth factor signaling (IIS) activity in the PG interferes with ecdysone production, indicating that IIS activity in the PG couples the nutritional state to development. However, the underlying mechanism is not well understood. In this study we show that the secreted Imaginal morphogenesis protein-Late 2 (Imp-L2), a growth inhibitor in Drosophila, is involved in this process. Imp-L2 inhibits the activity of the Drosophila insulin-like peptides by direct binding and is expressed by specific cells in the brain, the ring gland, the gut and the fat body. We demonstrate that Imp-L2 is required to regulate and adapt developmental timing to nutritional conditions by regulating IIS activity in the PG. Increasing Imp-L2 expression at its endogenous sites using an Imp-L2-Gal4 driver delays pupariation, while Imp-L2 mutants exhibit a slight acceleration of development. These effects are strongly enhanced by starvation and are accompanied by massive alterations of ecdysone production resulting most likely from increased Imp-L2 production by neurons directly contacting the PG and not from elevated Imp-L2 levels in the hemolymph. Taken together our results suggest that Imp-L2-expressing neurons sense the nutritional state of Drosophila larvae and coordinate dietary information and ecdysone production to adjust developmental timing under starvation conditions. Copyright © 2013 Elsevier Inc. All rights reserved.

cIMP synthesized by sGC as a mediator of hypoxic contraction of coronary arteries.

PubMed

Chen, Zhengju; Zhang, Xu; Ying, Lei; Dou, Dou; Li, Yanhui; Bai, Yun; Liu, Juan; Liu, Limei; Feng, Han; Yu, Xiaoxing; Leung, Susan Wai-Sum; Vanhoutte, Paul M; Gao, Yuansheng

2014-08-01

cGMP is considered the only mediator synthesized by soluble guanylyl cyclase (sGC) in response to nitric oxide (NO). However, purified sGC can synthesize several other cyclic nucleotides, including inosine 3',5'-cyclic monophosphate (cIMP). The present study was designed to determine the role of cIMP in hypoxic contractions of isolated porcine coronary arteries. Vascular responses were examined by measuring isometric tension. Cyclic nucleotides were assayed by HPLC tandem mass spectroscopy. Rho kinase (ROCK) activity was determined by measuring the phosphorylation of myosin phosphatase target subunit 1 using Western blot analysis and an ELISA kit. The level of cIMP, but not that of cGMP, was elevated by hypoxia in arteries with, but not in those without, endothelium [except if treated with diethylenetriamine (DETA) NONOate]; the increases in cIMP were inhibited by the sGC inhibitor 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ). Hypoxia (Po2: 25-30 mmHg) augmented contractions of arteries with and without endothelium if treated with DETA NONOate; these hypoxic contractions were blocked by ODQ. In arteries without endothelium, hypoxic augmentation of contraction was also obtained with exogenous cIMP. In arteries with endothelium, hypoxic augmentation of contraction was further enhanced by inosine 5'-triphosphate, the precursor for cIMP. The augmentation of contraction caused by hypoxia or cIMP was accompanied by increased phosphorylation of myosin phosphatase target subunit 1 at Thr(853), which was prevented by the ROCK inhibitor Y-27632. ROCK activity in the supernatant of isolated arteries was stimulated by cIMP in a concentration-dependent fashion. These results demonstrate that cIMP synthesized by sGC is the likely mediator of hypoxic augmentation of coronary vasoconstriction, in part by activating ROCK. Copyright © 2014 the American Physiological Society.

Imp and Syp RNA-binding proteins govern decommissioning of Drosophila neural stem cells

PubMed Central

Yang, Ching-Po; Samuels, Tamsin J.; Huang, Yaling; Yang, Lu; Ish-Horowicz, David; Davis, Ilan

2017-01-01

The termination of the proliferation of Drosophila neural stem cells, also known as neuroblasts (NBs), requires a ‘decommissioning’ phase that is controlled in a lineage-specific manner. Most NBs, with the exception of those of the mushroom body (MB), are decommissioned by the ecdysone receptor and mediator complex, causing them to shrink during metamorphosis, followed by nuclear accumulation of Prospero and cell cycle exit. Here, we demonstrate that the levels of Imp and Syp RNA-binding proteins regulate NB decommissioning. Descending Imp and ascending Syp expression have been shown to regulate neuronal temporal fate. We show that Imp levels decline slower in the MB than in other central brain NBs. MB NBs continue to express Imp into pupation, and the presence of Imp prevents decommissioning partly by inhibiting the mediator complex. Late-larval induction of transgenic Imp prevents many non-MB NBs from decommissioning in early pupae. Moreover, the presence of abundant Syp in aged NBs permits Prospero accumulation that, in turn, promotes cell cycle exit. Together, our results reveal that progeny temporal fate and progenitor decommissioning are co-regulated in protracted neuronal lineages. PMID:28851709

Pathfinder ground preparations prior to altitude record setting flight of 71,500 feet

NASA Technical Reports Server (NTRS)

1997-01-01

Technicians make final adjustments on the solar-powered Pathfinder remotely piloted research aircraft prior to the craft's taking off on a flight which established a new unofficial world's altitude record for both propellor-driven and solar-powered aircraft. The new record of more than 71,500 feet was set during a 14 1/2-hour flight July 7, 1997, from the U.S. Navy's Pacific Missile Range Facility (PMRF) at Barking Sands, Kauai, Hawaii. The new altitude record is subject to verification by the National Aeronautics Association. The Pathfinder took off at 8:34 a.m. HDT, passed its previous record altitude of 67,350 feet about 2:45 p.m., and then reached its new mark at about 4 p.m. Controllers on the ground then initiated a slow decent, and Pathfinder landed seven hours later at 11:05 p.m. HDT. The experimental Boeing Condor remotely-piloted aircraft had held the previous record for propellor-driven craft of 67,028 feet. The Pathfinder had exceeded that height on a previous flight on June 9, 1997, but not by a large enough margin to be considered a new record. Pathfinder was a lightweight, solar-powered, remotely piloted flying wing aircraft used to demonstrate the use of solar power for long-duration, high-altitude flight. Its name denotes its mission as the 'Pathfinder' or first in a series of solar-powered aircraft that will be able to remain airborne for weeks or months on scientific sampling and imaging missions. Solar arrays covered most of the upper wing surface of the Pathfinder aircraft. These arrays provided up to 8,000 watts of power at high noon on a clear summer day. That power fed the aircraft's six electric motors as well as its avionics, communications, and other electrical systems. Pathfinder also had a backup battery system that could provide power for two to five hours, allowing for limited-duration flight after dark. Pathfinder flew at airspeeds of only 15 to 20 mph. Pitch control was maintained by using tiny elevators on the trailing edge of the wing while turns and yaw control were accomplished by slowing down or speeding up the motors on the outboard sections of the wing. On September 11, 1995, Pathfinder set a new altitude record for solar-powered aircraft of 50,567 feet above Edwards Air Force Base, California, on a 12-hour flight. On July 7, 1997, it set another, unofficial record of 71,500 feet at the Pacific Missile Range Facility, Kauai, Hawaii. In 1998, Pathfinder was modified into the longer-winged Pathfinder Plus configuration. (See the Pathfinder Plus photos and project description.)

IMP-27, a Unique Metallo-β-Lactamase Identified in Geographically Distinct Isolates of Proteus mirabilis.

PubMed

Dixon, Nyssa; Fowler, Randal C; Yoshizumi, A; Horiyama, Tsukasa; Ishii, Y; Harrison, Lucas; Geyer, Chelsie N; Moland, Ellen Smith; Thomson, Kenneth; Hanson, Nancy D

2016-10-01

A novel metallo-β-lactamase gene, blaIMP-27, was identified in unrelated Proteus mirabilis isolates from two geographically distinct locations in the United States. Both isolates harbor blaIMP-27 as part of the first gene cassette in a class 2 integron. Antimicrobial susceptibility testing indicated susceptibility to aztreonam, piperacillin-tazobactam, and ceftazidime but resistance to ertapenem. However, hydrolysis assays indicated that ceftazidime was a substrate for IMP-27. Copyright © 2016 Dixon et al.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shirasaka, Y.; Ito, M.; Okuno, T.

Sequential {sup 123}I-N-isopropyl-p-iodoamphetamine (IMP) single-photon emission computed tomography (SPECT) was performed in 2 patients with acute infantile hemiplegia. In both patients, low uptake of IMP was detected in the targeted abnormal hemisphere. The {sup 123}I-IMP-SPECT findings indicative of a pathologic condition persisted even when the clinical findings and electroencephalographic abnormalities improved. Because of its sensitivity, noninvasiveness, and accurate reflection of the cerebral blood flow distribution, {sup 123}I-IMP-SPECT is useful in the examination of acute infantile hemiplegia and in the evaluation of prognosis.

Elucidating the Role of Residue 67 in IMP-Type Metallo-β-Lactamase Evolution.

PubMed

LaCuran, Alecander E; Pegg, Kevin M; Liu, Eleanor M; Bethel, Christopher R; Ai, Ni; Welsh, William J; Bonomo, Robert A; Oelschlaeger, Peter

2015-12-01

Antibiotic resistance in bacteria is ever changing and adapting, as once-novel β-lactam antibiotics are losing their efficacy, primarily due to the production of β-lactamases. Metallo-β-lactamases (MBLs) efficiently inactivate a broad range of β-lactam antibiotics, including carbapenems, and are often coexpressed with other antibacterial resistance factors. The rapid dissemination of MBLs and lack of novel antibacterials pose an imminent threat to global health. In an effort to better counter these resistance-conferring β-lactamases, an investigation of their natural evolution and resulting substrate specificity was employed. In this study, we elucidated the effects of different amino acid substitutions at position 67 in IMP-type MBLs on the ability to hydrolyze and confer resistance to a range of β-lactam antibiotics. Wild-type β-lactamases IMP-1 and IMP-10 and mutants IMP-1-V67A and IMP-1-V67I were characterized biophysically and biochemically, and MICs for Escherichia coli cells expressing these enzymes were determined. We found that all variants exhibited catalytic efficiencies (kcat/Km) equal to or higher than that of IMP-1 against all tested β-lactams except penicillins, against which IMP-1 and IMP-1-V67I showed the highest kcat/Km values. The substrate-specific effects of the different amino acid substitutions at position 67 are discussed in light of their side chain structures and possible interactions with the substrates. Docking calculations were employed to investigate interactions between different side chains and an inhibitor used as a β-lactam surrogate. The differences in binding affinities determined experimentally and computationally seem to be governed by hydrophobic interactions between residue 67 and the inhibitor and, by inference, the β-lactam substrates. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Characterization of the Eimeria maxima sporozoite surface protein IMP1.

PubMed

Jenkins, M C; Fetterer, R; Miska, K; Tuo, W; Kwok, O; Dubey, J P

2015-07-30

The purpose of this study was to characterize Eimeria maxima immune-mapped protein 1 (IMP1) that is hypothesized to play a role in eliciting protective immunity against E. maxima infection in chickens. RT-PCR analysis of RNA from unsporulated and sporulating E. maxima oocysts revealed highest transcription levels at 6-12h of sporulation with a considerable downregulation thereafter. Alignment of IMP1 coding sequence from Houghton, Weybridge, and APU-1 strains of E. maxima revealed single nucleotide polymorphisms that in some instances led to amino acid changes in the encoded protein sequence. The E. maxima (APU-1) IMP1 cDNA sequence was cloned and expressed in 2 different polyHis Escherichia coli expression vectors. Regardless of expression vector, recombinant E. maxima IMP1 (rEmaxIMP1) was fairly unstable in non-denaturing buffer, which is consistent with stability analysis of the primary amino acid sequence. Antisera specific for rEmaxIMP1 identified a single 72 kDa protein or a 61 kDa protein by non-reducing or reducing SDS-PAGE/immunoblotting. Immunofluorescence staining with anti-rEmaxIMP1, revealed intense surface staining of E. maxima sporozoites, with negligible staining of merozoite stages. Immuno-histochemical staining of E. maxima-infected chicken intestinal tissue revealed staining of E. maxima developmental stages in the lamnia propia and crypts at both 24 and 48 h post-infection, and negligible staining thereafter. The expression of IMP1 during early stages of in vivo development and its location on the sporozoite surface may explain in part the immunoprotective effect of this protein against E. maxima infection. Published by Elsevier B.V.

Hypoxic Vasospasm Mediated by cIMP: When Soluble Guanylyl Cyclase Turns Bad.

PubMed

Gao, Yuansheng; Chen, Zhengju; Leung, Susan W S; Vanhoutte, Paul M

2015-06-01

In a number of isolated blood vessel types, hypoxia causes an acute contraction that is dependent on the presence of nitric oxide and activation of soluble guanylyl cyclase. It is more pronounced when the preparations are constricted and is therefore termed hypoxic augmentation of vasoconstriction. This hypoxic response is accompanied by increases in the intracellular level of inosine 5'-triphosphate and in the synthesis of inosine 3',5'-cyclic monophosphate (cIMP) by soluble guanylyl cyclase. The administration of exogenous cIMP or inosine 5'-triphosphate causes augmented vasoconstriction to hypoxia. Furthermore, the vasoconstriction evoked by hypoxia and cIMP is associated with increased activity of Rho kinase (ROCK), indicating that cIMP may mediate the hypoxic effect by sensitizing the myofilaments to Ca through ROCK. Hypoxia is implicated in exaggerated vasoconstriction in the pathogenesis of coronary artery disease, myocardial infarction, hypertension, and stroke. The newly found role of cIMP may help to identify unique therapeutic targets for certain cardiovascular disorders.

Reflex test reminders in required cancer synoptic templates decrease order entry error: An analysis of mismatch repair immunohistochemical orders to screen for Lynch syndrome.

PubMed

Kilgore, Mark R; McIlwain, Carrie A; Schmidt, Rodney A; Norquist, Barbara M; Swisher, Elizabeth M; Garcia, Rochelle L; Rendi, Mara H

2016-01-01

Endometrial carcinoma (EC) is the most common extracolonic malignant neoplasm associated with Lynch syndrome (LS). LS is caused by autosomal dominant germline mutations in DNA mismatch repair (MMR) genes. Screening for LS in EC is often evaluated by loss of immunohistochemical (IHC) expression of DNA MMR enzymes MLH1, MSH2, MSH6, and PMS2 (MMR IHC). In July 2013, our clinicians asked that we screen all EC in patients ≤60 for loss of MMR IHC expression. Despite this policy, several cases were not screened or screening was delayed. We implemented an informatics-based approach to ensure that all women who met criteria would have timely screening. Reports are created in PowerPath (Sunquest Information Systems, Tucson, AZ) with custom synoptic templates. We implemented an algorithm on March 6, 2014 requiring pathologists to address MMR IHC in patients ≤60 with EC before sign out (S/O). Pathologists must answer these questions: is patient ≤60 (yes/no), if yes, follow-up questions (IHC done previously, ordered with addendum to follow, results included in report, N/A, or not ordered), if not ordered, one must explain. We analyzed cases from July 18, 2013 to August 31, 2016 preimplementation (PreImp) and postimplementation (PostImp) that met criteria. Data analysis was performed using the standard data package included with GraphPad Prism ® 7.00 (GraphPad Software, Inc., La Jolla, CA, USA). There were 147 patients who met criteria (29 PreImp and 118 PostImp). IHC was ordered in a more complete and timely fashion PostImp than PreImp. PreImp, 4/29 (13.8%) cases did not get any IHC, but PostImp, only 4/118 (3.39%) were missed ( P = 0.0448). Of cases with IHC ordered, 60.0% (15/25) were ordered before or at S/O PreImp versus 91.2% (104/114) PostImp ( P = 0.0004). Relative to day of S/O, the mean days of order delay were longer and more variable PreImp versus PostImp (12.9 ± 40.7 vs. -0.660 ± 1.15; P = 0.0227), with the average being before S/O PostImp. This algorithm ensures MMR IHC ordering in women ≤60 with EC and can be applied to similar scenarios. Ancillary tests for management are increasing, especially genetic and molecular-based methods. The burden of managing orders and results remains with the pathologist and relying on human intervention alone is ineffective. Ordering IHC before or at S/O prevents oversight and the additional work of retrospective ordering and reporting.

Reliability and concurrent validity of the Infant Motor Profile.

PubMed

Heineman, Kirsten R; Middelburg, Karin J; Bos, Arend F; Eidhof, Lieke; La Bastide-Van Gemert, Sacha; Van Den Heuvel, Edwin R; Hadders-Algra, Mijna

2013-06-01

The Infant Motor Profile (IMP) is a qualitative assessment of motor behaviour in infancy. It consists of five domains: movement variation, variability, fluency, symmetry, and performance. The aim of this study was to assess interobserver reliability and concurrent validity of the IMP with the Alberta Infant Motor Scale (AIMS) and an age-specific neurological examination. Fifty-nine preterm infants (25 females, 34 males; median gestational age 29.7wks, median birthweight 1285g) and 146 term infants (74 females, 72 males; median gestational age 40.1wks, birthweight 3500g) were included. Assessments were performed at corrected ages of 4, 6, 10, 12, and 18 months and consisted of the IMP, AIMS, and an age-specific neurological examination. Interobserver reliability was investigated on a sample of 25 video recordings. Non-parametric statistics were used to analyse the data. Interobserver reliability was high (intraclass correlation coefficient 0.95). At all ages, AIMS scores correlated weakly to fairly with total IMP scores (Spearman's ρ 0.36-0.55), but moderately to strongly with scores on the performance domain of the IMP (Spearman's ρ 0.47-0.84). A clear relation was found between total IMP score and outcome of the neurological examination (Kruskal-Wallis p<0.001 at all ages). Interobserver reliability of the IMP is good. Concurrent validity with the AIMS is best for the IMP performance domain. Concurrent validity with age-specific neurological examination is very good. © The Authors. Developmental Medicine & Child Neurology © 2013 Mac Keith Press.

Characterization of Protein Detergent Complexes by NMR, Light Scattering, and Analytical Ultracentrifugation

PubMed Central

Maslennikov, Innokentiy; Krupa, Martin; Dickson, Christopher; Esquivies, Luis; Blain, Katherine; Kefala, Georgia; Choe, Senyon; Kwiatkowski, Witek

2009-01-01

Bottlenecks in expression, solubilization, purification and crystallization hamper the structural study of integral membrane proteins (IMPs). Successful crystallization is critically dependent on the purity, stability and oligomeric homogeneity of an IMP sample. These characteristics are in turn strongly influenced by the type and concentration of the detergents used in IMP preparation. By utilizing the techniques and analytical tools we earlier developed for the characterization of protein-detergent complexes (PDCs) (Maslennikov et al., 2007), we demonstrate that for successful protein extraction from E. coli membrane fractions, the solubilizing detergent associates preferentially to IMPs rather than to membrane lipids. Notably, this result is contrary to the generally accepted mechanism of detergent-mediated IMP solubilization. We find that for one particular member of the family of proteins studied (E. coli receptor kinases, which is purified in mixed multimeric states and oligomerizes through its transmembrane region), the protein oligomeric composition is largely unaffected by a 10-fold increase in protein concentration, by alteration of micelle properties through addition of other detergents to the PDC sample, or by a 20-fold variation in the detergent concentration used for solubilization of the IMP from the membrane. We observed that the conditions used for expression of the IMP, which impact protein density in the membrane, has the greatest influence on the IMP oligomeric structure. Finally, we argue that for concentrating PDCs smaller than 30 kDa, stirred concentration cells are less prone to over-concentration of detergent and are therefore more effective than centrifugal ultrafiltration devices. PMID:19214777

Micro-scale NMR Experiments for Monitoring the Optimization of Membrane Protein Solutions for Structural Biology.

PubMed

Horst, Reto; Wüthrich, Kurt

2015-07-20

Reconstitution of integral membrane proteins (IMP) in aqueous solutions of detergent micelles has been extensively used in structural biology, using either X-ray crystallography or NMR in solution. Further progress could be achieved by establishing a rational basis for the selection of detergent and buffer conditions, since the stringent bottleneck that slows down the structural biology of IMPs is the preparation of diffracting crystals or concentrated solutions of stable isotope labeled IMPs. Here, we describe procedures to monitor the quality of aqueous solutions of [ 2 H, 15 N]-labeled IMPs reconstituted in detergent micelles. This approach has been developed for studies of β-barrel IMPs, where it was successfully applied for numerous NMR structure determinations, and it has also been adapted for use with α-helical IMPs, in particular GPCRs, in guiding crystallization trials and optimizing samples for NMR studies (Horst et al ., 2013). 2D [ 15 N, 1 H]-correlation maps are used as "fingerprints" to assess the foldedness of the IMP in solution. For promising samples, these "inexpensive" data are then supplemented with measurements of the translational and rotational diffusion coefficients, which give information on the shape and size of the IMP/detergent mixed micelles. Using microcoil equipment for these NMR experiments enables data collection with only micrograms of protein and detergent. This makes serial screens of variable solution conditions viable, enabling the optimization of parameters such as the detergent concentration, sample temperature, pH and the composition of the buffer.

The semantic pathfinder: using an authoring metaphor for generic multimedia indexing.

PubMed

Snoek, Cees G M; Worring, Marcel; Geusebroek, Jan-Mark; Koelma, Dennis C; Seinstra, Frank J; Smeulders, Arnold W M

2006-10-01

This paper presents the semantic pathfinder architecture for generic indexing of multimedia archives. The semantic pathfinder extracts semantic concepts from video by exploring different paths through three consecutive analysis steps, which we derive from the observation that produced video is the result of an authoring-driven process. We exploit this authoring metaphor for machine-driven understanding. The pathfinder starts with the content analysis step. In this analysis step, we follow a data-driven approach of indexing semantics. The style analysis step is the second analysis step. Here, we tackle the indexing problem by viewing a video from the perspective of production. Finally, in the context analysis step, we view semantics in context. The virtue of the semantic pathfinder is its ability to learn the best path of analysis steps on a per-concept basis. To show the generality of this novel indexing approach, we develop detectors for a lexicon of 32 concepts and we evaluate the semantic pathfinder against the 2004 NIST TRECVID video retrieval benchmark, using a news archive of 64 hours. Top ranking performance in the semantic concept detection task indicates the merit of the semantic pathfinder for generic indexing of multimedia archives.

Kinetic analysis of IMP split dose method for two consecutive measurement of cerebral blood flow

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nishizawa, S.; Yonekura, Y.; Tanaka, F.

1994-05-01

The split dose method for two consecutive measurements of cerebral blood flow (CBF) with I-123 IMP seems to offer a great merit to the SPECT study of the brain. However, because of complexity of the dynamics of IMP, it is not clear if microsphere (MS) model permits a estimation of CBF for the 2nd dose. We applied kinetic (KN) analysis based on 2 compartment model to the dynamic SPECT scan data, and compared the results with those obtained by MS model. Dynamic SPECT (1-min scans for 50 min) was performed using 3-head SPECT camera in 5 patients to test themore » reproducibility of measured CBF and in 9 patients to test the vascular response to acetazolamide (ACZ). Two doses of IMP (111 MBq each) were injected at the time of, and 25 min after, the scan initiation. ACZ (1g) was administered at 13 min. Arterial blood samples were drawn manually during the scan and an octanol extracted input function was obtained. Dynamic scan data for 22 min was used for CBF by KN analysis (K1), and 4-min scan data at 5 min for CBF by MS model (Km), for each dose. For 2nd CBF by MS model, simple subtraction of brain activity due to the I st dose was done using 4-min scan data just prior to the 2nd dose. Reproducibility of measured CBF by KN analysis was excellent (r=0.949, 1st K1=39.2{plus_minus}5.6 and 2nd K1=38.5{plus_minus}6.6 ml/l00g/min: mean{plus_minus}SD). Vascular response to ACZ was good (1st K1=42.4{plus_minus}7.8 to 2nd K1=67.9{plus_minus}10.0) in areas without ischemia, but poor (1st K1=41.1{plus_minus}8.5 to 2nd K1=46.1{plus_minus}11.1) in ischemic areas. Compared to KN analysis, MS model underestimated 3.5% for the 1st CBF measurement and 12.8% for the 2nd. However, excellent correlation was observed not only between 1st K1 and Km (r=0.993, slope=0.920) but between 2nd K1 and Km (r=0.994, slope=0.814), and the results permitted a reasonable correction for Km.« less

Navigation Flight Operations for Mars Pathfinder

NASA Technical Reports Server (NTRS)

Vaughan, Robin M.; Kallemeyn, Pieter H., Jr.; Spencer, David A.; Braun, Robert D.

2004-01-01

On July 4, 1997, Mars Pathfinder became the first spacecraft to land on the surface of Mars in 21 years. Pathfinder was launched on December 4, 1996 and spent seven months en route to the red planet. This report discusses the navigation flight experience for the Mars Pathfinder interplanetary cruise. In particular, orbit determination and maneuver design and execution results are presented. Special emphasis is given to the navigation role in the days and hours leading up to and including the Entry, Descent, and Landing (EDL) phase.

Validating the Usefulness of Combined Japanese GMS Data For Long-Term Global Change Studies

NASA Technical Reports Server (NTRS)

Simpson, James J.; Dodge, James C. (Technical Monitor)

2001-01-01

The primary objectives of the Geostationary Meteorological Satellite (GMS)-5 Pathfinder Project were the following: (1) to evaluate GMS-5 data for sources of error and develop methods for minimizing any such errors in GMS-5 data; (2) to prepare a GMS-5 Pathfinder data set for the GMS-5 Pathfinder Benchmark Period (1 July 95 - 30 June 96); and (3) show the usefulness of the improved Pathfinder data set in at least one geophysical application. All objectives were met.

IMP 7 (Explorer 47) trajectory, September 26, 1972 to September 25, 1978

NASA Technical Reports Server (NTRS)

Milligan, Pamela A.; Lazarus, Alan J.

1988-01-01

The trajectory plots for IMP 7 (Explorer 47) are contained. For each orbit the trajectory is shown in five panels on two pages; each panel is a different representation or projection. The trajectory parameters were obtained from the multi-coordinate ephemeris (MCE) tapes supplied to IMP experimenters by the IMP project. The plots on the right hand pages use a geocentric, solar-ecliptic coordinate system. Distances are in units of earth radii. The plots on the left hand pages use geocentric, solar magnetospheric coordinates with distances in earth radii.

Imp2 regulates GBM progression by activating IGF2/PI3K/Akt pathway.

PubMed

Mu, Qingchun; Wang, Lijun; Yu, Fengbo; Gao, Haijun; Lei, Ting; Li, Peiwen; Liu, Pengfei; Zheng, Xu; Hu, Xitong; Chen, Yong; Jiang, Zhenfeng; Sayari, Arash J; Shen, Jia; Huang, Haiyan

2015-01-01

Glioblastomas multiforme (GBM) are the most frequently occurring malignant brain cancers. Treatment for GBM consists of surgical resection and subsequent adjuvant radiation therapy and chemotherapy. Despite this, GBM patient survival is limited to 12-15 months, and researchers are continually trying to develop improved therapy options. Insulin-like growth factor 2 mRNA-binding protein 2 (Imp2) is known to be upregulated in many cancers and is known to regulate the signaling activity of insulin-like growth factor 2 (IGF2). However, relatively little is known about its role in malignant development of GBM. In this study, we first found Imp2 is upregulated in GBM tissues by using clinical samples and public database search. Studies with loss and gain of Imp2 expression in in vitro GBM cell culture system demonstrated the role of Imp2 in promoting GBM cell proliferation, migration, invasion and epithelial-to-mesenchymal transition (EMT). Additionally, our results show that Imp2 regulates the activity of IGF2, which further activates PI3K/Akt signaling, thereby to promote GBM malignancy. Inhibition of Imp2 was also found to sensitize GBM to temozolomide treatment. These observations add to the current knowledge of GBM biology, and may prove useful in development of more effective GBM therapy.

Imp2 regulates GBM progression by activating IGF2/PI3K/Akt pathway

PubMed Central

Mu, Qingchun; Wang, Lijun; Yu, Fengbo; Gao, Haijun; Lei, Ting; Li, Peiwen; Liu, Pengfei; Zheng, Xu; Hu, Xitong; Chen, Yong; Jiang, Zhenfeng; Sayari, Arash J; Shen, Jia; Huang, Haiyan

2015-01-01

Glioblastomas multiforme (GBM) are the most frequently occurring malignant brain cancers. Treatment for GBM consists of surgical resection and subsequent adjuvant radiation therapy and chemotherapy. Despite this, GBM patient survival is limited to 12–15 months, and researchers are continually trying to develop improved therapy options. Insulin-like growth factor 2 mRNA-binding protein 2 (Imp2) is known to be upregulated in many cancers and is known to regulate the signaling activity of insulin-like growth factor 2 (IGF2). However, relatively little is known about its role in malignant development of GBM. In this study, we first found Imp2 is upregulated in GBM tissues by using clinical samples and public database search. Studies with loss and gain of Imp2 expression in in vitro GBM cell culture system demonstrated the role of Imp2 in promoting GBM cell proliferation, migration, invasion and epithelial-to-mesenchymal transition (EMT). Additionally, our results show that Imp2 regulates the activity of IGF2, which further activates PI3K/Akt signaling, thereby to promote GBM malignancy. Inhibition of Imp2 was also found to sensitize GBM to temozolomide treatment. These observations add to the current knowledge of GBM biology, and may prove useful in development of more effective GBM therapy. PMID:25719943

IMP3 can predict aggressive behaviour of lung adenocarcinoma

PubMed Central

2012-01-01

Background Lung cancer most often presents as an inoperable tumour and the diagnosis is usually performed on a small biopsy/cytology specimen. In the group of non small cell lung cancer - not otherwise specified, adenocarcinoma phenotype can be determined immunohistochemically using TTF-1 and Napsin A. Expression of oncofetal protein IMP3 in human cancer is associated with poor differentiation and aggressive behaviour. In the present study expression of IMP3 was correlated with expression of TTF-1 and Napsin A, histological subtype and clinical stage of lung adenocarcinoma. We were interested whether distant metastases are associated with IMP3 overexpression, regardless of the histologic subtype of adenocarcinoma. Methods In retrospective study, consecutive series of 105 patients with advanced lung adenocarcinoma diagnosed from 2006 to 2009 in Clinical Hospital Center Split, Croatia, were analysed. Clinical data were collected from the Pulmology Department and time of death from the Mortality Registry. Paraffin blocks of bronchoscopic biopsies were collected from the Institute of Pathology and 15 cases excluded from the analysis due to insufficient material. Expression of IMP3, Napsin A and TTF-1 were analysed by indirect enzyme immunohistochemistry. Statistical analysis was performed and P values less than 0.05 considered significant. Results Of 90 patients, 71 (78%) were males and 19 (22%) females. Median age for males was 61.5 years (min-max 43–83) and for females 61 years (min-max 44–86). Pleural effusion was found in 15 (16.6%) and distant metastases in 45 (50%) cases. According to histological subtypes, there were 34 acinar, 2 lepidic, 2 papillary and 52 solid subtypes. IMP3 overexpression was found in 63 cases (70%) and was correlated with solid subtype (P = 0.002) and negative/weak Napsin A expression (P = 0.004). Strong Napsin A expression correlated with TTF-1 expression (P = 0.003) and lower histological grades (P = 0.031). Patients with IMP3 overexpression more often had distant metastases than patients with negative IMP3, 55.5% versus 33.3% (P = 0.033). Non solid subtypes with IMP3 overexpression developed distant metastasis more common than non solid subtypes with negative IMP3, 72% versus 35% (P = 0.028). Conclusions Expression of IMP3 correlates with solid subtype and with distant metastases regardless of histological subtype of lung adenocarcinoma. Virtual slides http://www.diagnosticpathology.diagnomx.eu/vs/1966211581795258 Zusammenfassung Hintergrund Das Lungenkarzinom kommt meistens als nicht resektabler Tumor vor und die Diagnose kann nur in kleinen Biopsaten oder zytologisch gestellt werden. In der Gruppe der nicht kleinzelligen Lungenkarzinome kann der nicht anders spezifizierte Adenokarzinom Phänotyp mit Hilfe der Antikörper TTF-1 und Napsin A diagnostiziert werden. Die Expression des onkoföetalen Proteins IMP3 ist bei humanen Karzinomen mit agressivem Verhalten und metastatischem Potential verbunden. In dieser Studie korreliert die Expression von IMP3 mit TTF-1, Napsin A, histologischem Typ und klinischem Staging des Lungenkarzinoms. Wir waren daran interessiert, ob Fernmetastasen mit IMP3 Überexpression assoziiert sind, unabhängig von der histologischen Subtyp von Adenokarzinom. Methode In der retrospektiven Studie wurden die von 2006 bis 2009 im Klinischem Krankenhaus Split, Kroatien diagnostizerte Adenokarzinome der Lunge von 105 Patienten analysiert. Die klinischen Daten stammten aus der Abteilung für Pulmologie und im Falle des Todes vom Todesregister. Die Paraffinblöcke der primären Lungenbiopsate dieser Patienten wurden im Institut für Pathologie mit der indirekter Enzym - Immunohistochemie mittels Kombination der Antikörper gegen IMP3, Napsin A und TTF1 untersucht. 15 Fälle aus der Analyse aufgrund unzureichender Material ausgeschlossen. Es wurde eine statistische Untersuchung durchgeführt und Werte weniger als 0.05 P wurden als statistisch signifikant bezeichnet. Ergebnisse Von 90 Patienten mit Lungencarcinom waren 71 (78%) mänlich, durchschnittliches Alter war für Männer 61.5 Jahre (min-max 43–83) und 61 Jahre für Frauen (min-max 44–86). Pleurale Effusionen fand man in 15 Fällen (16.6%) und Fernmetastasen in 45 (50%) Fällen. Histologische Sybtypen waren: 2 lepidic Karzinome, 34 azinäre Karzinome, 2 papilläre und 52 solide Karzinome. IMP3 war exprimiert in 63 Fälle (70%). Positive IMP3 Expression war mit solidem Typ (P = 0.002) und negativer Napsin A Expression (P = 0.004) assoziert. Napsin A Expression war mit niedrigem Gradus (P = 0.031) und positiver TTF-1 Expression (P = 0.003) assoziert. Patienten mit IMP3 Überexpression öfter hatten Fernmetastasen als Patienten mit negativen IMP3, 55.5% versus 33.3% (P = 0.033). Non solide Subtyp mit IMP3 Überexpression entwickelten Fernmetastasen Meer häufiger als nicht festem Subtyp mit negativen IMP3, 72% versus 35% (P = 0.028). Schlussworte Die Expression von IMP3 ist mit negaativer Expression von Napsin A, solidem Subtyp und Metastasen verbunden und hat praktische predictive Werte in der pathologischen Diagnose des Adenokarzinoms der Lunge. Die Expression von IMP3 korreliert mit soliden Subtyp und mit Fernmetastasen unabhängig von histologische Subtyp Lungenadenokarzinom. PMID:23190601

Robot Manipulator Technologies for Planetary Exploration

NASA Technical Reports Server (NTRS)

Das, H.; Bao, X.; Bar-Cohen, Y.; Bonitz, R.; Lindemann, R.; Maimone, M.; Nesnas, I.; Voorhees, C.

1999-01-01

NASA exploration missions to Mars, initiated by the Mars Pathfinder mission in July 1997, will continue over the next decade. The missions require challenging innovations in robot design and improvements in autonomy to meet ambitious objectives under tight budget and time constraints. The authors are developing design tools, component technologies and capabilities to address these needs for manipulation with robots for planetary exploration. The specific developments are: 1) a software analysis tool to reduce robot design iteration cycles and optimize on design solutions, 2) new piezoelectric ultrasonic motors (USM) for light-weight and high torque actuation in planetary environments, 3) use of advanced materials and structures for strong and light-weight robot arms and 4) intelligent camera-image coordinated autonomous control of robot arms for instrument placement and sample acquisition from a rover vehicle.

Polarimetric Observations of the Lunar Surface

NASA Astrophysics Data System (ADS)

Kim, S.

2017-12-01

Polarimetric images contain valuable information on the lunar surface such as grain size and porosity of the regolith, from which one can estimate the space weathering environment on the lunar surface. Surprisingly, polarimetric observation has never been conducted from the lunar orbit before. A Wide-Angle Polarimetric Camera (PolCam) has been recently selected as one of three Korean science instruments onboard the Korea Pathfinder Lunar Orbiter (KPLO), which is aimed to be launched in 2019/2020 as the first Korean lunar mission. PolCam will obtain 80 m-resolution polarimetric images of the whole lunar surface between -70º and +70º latitudes at 320, 430 and 750 nm bands for phase angles up to 115º. I will also discuss previous polarimetric studies on the lunar surface based on our ground-based observations.

Testing of the LISA pathfinder GRS

NASA Astrophysics Data System (ADS)

Antonucci, Federica; Cavalleri, Antonella; Ciani, Giacomo; Congedo, Giuseppe; Dolesi, Rita; de Marchi, Fabrizio; Ferraioli, Luigi; Hueller, Mauro; Nicolodi, Daniele; Tombolato, David; Vitale, Stefano; Wass, Peter J.; Weber, William J.

The ESA/NASA mission,LISA (Laser Interferometric Space Antenna), will measure gravita-tional waves emitted by astronomical sources, galactic and extra-galactic, at frequencies 10-4 to 10-1 Hz. LISA is a 5-million-km arm-length interferometer whose mirrors are test masses which must be nominally free-falling to a level which does not exceed 3 · 10-15 ms-2 Hz -1/2 in acceleration. LISA Pathfinder is a technology demonstration mission which will show that the relative parasitic acceleration between two masses on one spacecraft can be lower than 3 · 10-14 ms-2 Hz -1/2 , at frequencies around 1 mHz -one order of magnitude larger than LISA's goal. At the core of the LISA Pathfinder experiment is the GRS (gravitational reference sensor), a capacitive sensor with mm gaps used to measure the position of the test mass and actuate its position in 6-degrees-of-freedom. Testing the purity of free-fall for LISA Pathfinder on-ground is achieved using a torsion pendulum which allows us to measure force disturbances at a level relevant to LISA Pathfinder. We will present the latest campaign of tests of the LISA Pathfinder GRS using the 4-test-mass torsion pendulum facility aimed at measuring force-noise sources (responsible for the parasitic acceleration) for LISA Pathfinder in its frequency band. Our GRS , is the LISA Pathfinder flight-model replica, and its testing is crucial in verifying the design and performance of the flight instrument and measuring many of the unwanted disturbances which can limit the performance of LISA and LISA pathfinder. The measurements concern the dependence of the force on the test mass position in the sensor and their electrostatic coupling, electrostatic fields due to surface-potential variations and thermal gradients.

Space Science

NASA Image and Video Library

1996-12-04

The Mars Pathfinder began the journey to Mars with liftoff atop a Delta II expendable launch vehicle from launch Complex 17B on Cape Canaveral Air Station. The Mars Pathfinder traveled on a direct trajectory to Mars, and arrived there in July 1997. Mars Pathfinder sent a lander and small robotic rover, Sojourner, to the surface of Mars. The primary objective of the mission was to demonstrate a low-cost way of delivering a science package to the surface of Mars using a direct entry, descent and landing with the aid of small rocket engines, a parachute, airbags and other techniques. In addition, landers and rovers of the future will share the heritage of Mars Pathfinder designs and technologies first tested in this mission. Pathfinder also collected invaluable data about the Martian surface.

Identification of metabolic pathways using pathfinding approaches: a systematic review.

PubMed

Abd Algfoor, Zeyad; Shahrizal Sunar, Mohd; Abdullah, Afnizanfaizal; Kolivand, Hoshang

2017-03-01

Metabolic pathways have become increasingly available for various microorganisms. Such pathways have spurred the development of a wide array of computational tools, in particular, mathematical pathfinding approaches. This article can facilitate the understanding of computational analysis of metabolic pathways in genomics. Moreover, stoichiometric and pathfinding approaches in metabolic pathway analysis are discussed. Three major types of studies are elaborated: stoichiometric identification models, pathway-based graph analysis and pathfinding approaches in cellular metabolism. Furthermore, evaluation of the outcomes of the pathways with mathematical benchmarking metrics is provided. This review would lead to better comprehension of metabolism behaviors in living cells, in terms of computed pathfinding approaches. © The Author 2016. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Mars Pathfinder Atmospheric Entry Navigation Operations

NASA Technical Reports Server (NTRS)

Braun, R. D.; Spencer, D. A.; Kallemeyn, P. H.; Vaughan, R. M.

1997-01-01

On July 4, 1997, after traveling close to 500 million km, the Pathfinder spacecraft successfully completed entry, descent, and landing, coming to rest on the surface of Mars just 27 km from its target point. In the present paper, the atmospheric entry and approach navigation activities required in support of this mission are discussed. In particular, the flight software parameter update and landing site prediction analyses performed by the Pathfinder operations navigation team are described. A suite of simulation tools developed during Pathfinder's design cycle, but extendible to Pathfinder operations, are also presented. Data regarding the accuracy of the primary parachute deployment algorithm is extracted from the Pathfinder flight data, demonstrating that this algorithm performed as predicted. The increased probability of mission success through the software parameter update process is discussed. This paper also demonstrates the importance of modeling atmospheric flight uncertainties in the estimation of an accurate landing site. With these atmospheric effects included, the final landed ellipse prediction differs from the post-flight determined landing site by less then 0.5 km in downtrack.

Dimensions of disinhibited personality and their relation with alcohol use and problems

PubMed Central

Gunn, Rachel L.; Finn, Peter R.; Endres, Michael J.; Gerst, Kyle R.; Spinola, Suzanne

2013-01-01

Although alcohol use disorders (AUDs) have been associated with different aspects of disinhibited personality and antisociality, less is known about the specific relationships among different domains of disinhibited personality, antisociality, alcohol use, and alcohol problems. The current study was designed to address three goals, (i) to provide evidence of a three-factor model of disinhibited personality (comprised of impulsivity [IMP], risk taking/ low harm avoidance [RTHA], excitement seeking [ES]), (ii) to test hypotheses regarding the association between each dimension and alcohol use and problems, and (iii) to test the hypothesis that antisociality (social deviance proneness [SDP]) accounts for the direct association between IMP and alcohol problems, while ES is directly related to alcohol use. Measures of disinhibited personality IMP, RTHA, ES and SDP and alcohol use and problems were assessed in a sample of young adults (N=474), which included a high proportion of individuals with AUDs. Confirmatory factor analyses supported a three-factor model of disinhibited personality reflecting IMP, RTHA, and ES. A structural equation model (SEM) showed that IMP was specifically associated with alcohol problems, while ES was specifically associated with alcohol use. In a second SEM, SDP accounted for the majority of the variance in alcohol problems associated with IMP. The results suggest aspects of IMP associated with SDP represent a direct vulnerability to alcohol problems. In addition, the results suggest that ES reflects a specific vulnerability to excessive alcohol use, which is then associated with alcohol problems, while RTHA is not specifically associated with alcohol use or problems when controlling for IMP and ES. PMID:23588138

Integrating Subject Pathfinders into Online Catalogs.

ERIC Educational Resources Information Center

Jarvis, William E.

1985-01-01

Discusses the integration of subject pathfinders into online public access catalogs (OPAC) through following features: within the OPAC, offline user guide manuals, remotely printed upon user request, or online as saved searches displayed in help screen format. Excerpts of a pathfinder display for biotechnology are presented. Four sources are…

Parallel algorithms for islanded microgrid with photovoltaic and energy storage systems planning optimization problem: Material selection and quantity demand optimization

NASA Astrophysics Data System (ADS)

Cao, Yang; Liu, Chun; Huang, Yuehui; Wang, Tieqiang; Sun, Chenjun; Yuan, Yue; Zhang, Xinsong; Wu, Shuyun

2017-02-01

With the development of roof photovoltaic power (PV) generation technology and the increasingly urgent need to improve supply reliability levels in remote areas, islanded microgrid with photovoltaic and energy storage systems (IMPE) is developing rapidly. The high costs of photovoltaic panel material and energy storage battery material have become the primary factors that hinder the development of IMPE. The advantages and disadvantages of different types of photovoltaic panel materials and energy storage battery materials are analyzed in this paper, and guidance is provided on material selection for IMPE planners. The time sequential simulation method is applied to optimize material demands of the IMPE. The model is solved by parallel algorithms that are provided by a commercial solver named CPLEX. Finally, to verify the model, an actual IMPE is selected as a case system. Simulation results on the case system indicate that the optimization model and corresponding algorithm is feasible. Guidance for material selection and quantity demand for IMPEs in remote areas is provided by this method.

IMP improves water-holding capacity, physical and sensory properties of heat-induced gels from porcine meat.

PubMed

Nakamura, Yukinobu; Migita, Koshiro; Okitani, Akihiro; Matsuishi, Masanori

2014-05-01

Water-holding capacity (WHC) of heat-induced pork gels was examined. The heat-induced gels were obtained from meat homogenates prepared by adding nine volumes of 0.3-0.5 mol/L NaCl solutions containing 9-36 mmol/L disodium inosine-5'-monophosphate (IMP) or 9 mmol/L tetrapotassium pyrophosphate (KPP) to minced pork. IMP at 36 mmol/L enhanced the WHC to the same level as attained by KPP. Physical and sensory properties of heat-induced gels were also examined. The heat-induced gels were prepared from porcine meat homogenates containing 0.3 mol/L NaCl and 9-36 mmol/L IMP or 9 mmol/L KPP. IMP at 36 mmol/L enhanced the values of hardness, cohesiveness, gumminess and springiness, measured with a Tensipresser, and several organoleptic scores to the same level as the score attained by KPP. Thus, it is concluded that IMP is expected to be a practical substitute for pyrophosphates to improve the quality of sausages. © 2014 Japanese Society of Animal Science.

Affinity of guanosine derivatives for polycytidylate revisited

NASA Technical Reports Server (NTRS)

Kanavarioti, A.; Hurley, T. B.; Baird, E. E.

1995-01-01

Evidence is presented for complexation of guanosine 5'-monophosphate 2-methylimidazolide (2-MeImpG) with polycytidylate (poly(C)) at pH 8.0 and 23 degrees C in the presence of 1.0 M NaCl2 and 0.2 M MgCl2 in water. The association of 2-MeImpG with poly(C) was investigated using UV-vis spectroscopy as well as by monitoring the kinetics of the nucleophilic substitution reaction of the imidazole moiety by amines. The results of both methods are consistent with moderately strong poly(C) 2-MeImpG complexation and the spectrophotometric measurements allowed the construction of a binding isotherm with a concentration of 2-MeImpG equal to 5.55 +/- 0.15 mM at half occupancy. UV spectroscopy was employed to establish the binding of other guanosine derivatives on poly(C). These derivatives are guanosine 5'-monophosphate (5'GMP), guanosine 5'-monophosphate imidazolide (ImpG), and guanosine 5'-monophosphate morpholidate (morpG). Within experimental error these guanosine derivatives exhibit the same affinity for poly(C) as 2-MeImpG.

Dominance of IMP-4-Producing Enterobacter cloacae among Carbapenemase-Producing Enterobacteriaceae in Australia

PubMed Central

Townell, Nicola; Nimmo, Graeme R.; George, Narelle M.; Robson, Jennifer; Vohra, Renu; Davis, Louise; Heney, Claire; Paterson, David L.

2015-01-01

The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) has been increasing worldwide. blaIMP has been reported to be the predominant carbapenemase-encoding gene within Enterobacteriaceae in Australia. However, there are limited data currently available on CPE from Queensland, Australia. A total of 58 CPE isolates were isolated between July 2009 and March 2014 from Queensland hospitals. The clonality of isolates was determined by Diversilab repetitive sequence-based PCR. The isolates were investigated for the resistance mechanisms carbapenemase, extended-spectrum β-lactamase, and AmpC β-lactamase and for aminoglycoside resistance and plasmid-mediated quinolone resistance genes by PCR. The plasmid types associated with carbapenemase-encoding genes were characterized. The majority of the CPE were Enterobacter cloacae (n = 29). The majority of Queensland CPE isolates were IMP producers and comprised 11 species (n = 48). Nine NDM-producing Enterobacteriaceae were identified. One NDM-producing Klebsiella pneumoniae isolate coproduced OXA-48. One K. pneumoniae isolate was an OXA-181 producer. The incidence of IMP producers increased significantly in 2013. blaIMP-4 was found in all IMP-producing isolates. blaTEM, qnrB, and aacA4 were common among IMP-4 producers. The HI2 (67%) and L/M (21%) replicons were associated with blaIMP-4. All HI2 plasmids were of sequence type 1 (ST1). All but one of the NDM producers possessed blaCTX-M-15. The 16S rRNA methylase genes found among NDM producers were armA, rmtB, rmtC, and rmtF. The substantial increase in the prevalence of CPE in Queensland has been associated mainly with the emergence E. cloacae strains possessing HI2 plasmids carrying blaIMP-4 over the past 2 years. The importation of NDM producers and/or OXA-48-like producers in patients also contributed to the increased emergence of CPE. PMID:25918153

What does the brain of children with developmental dyslexia tell us about reading improvement? ERP evidence from an intervention study

PubMed Central

Hasko, Sandra; Groth, Katarina; Bruder, Jennifer; Bartling, Jürgen; Schulte-Körne, Gerd

2014-01-01

Intervention is key to managing developmental dyslexia (DD), but not all children with DD benefit from treatment. Some children improve (improvers, IMP), whereas others do not improve (non-improvers, NIMP). Neurobiological differences between IMP and NIMP have been suggested, but studies comparing IMP and NIMP in childhood are missing. The present study examined whether ERP patterns change with treatment and differ between IMP and NIMP. We investigated the ERPs of 28 children with DD and 25 control children (CON) while performing a phonological lexical decision (PLD) task before and after a 6-month intervention. After intervention children with DD were divided into IMP (n = 11) and NIMP (n = 17). In the PLD–task children were visually presented with words, pseudohomophones, pseudowords, and false fonts and had to decide whether the presented stimulus sounded like an existing German word or not. Prior to intervention IMP showed higher N300 amplitudes over fronto-temporal electrodes compared to NIMP and CON and N400 amplitudes were attenuated in both IMP and NIMP compared to CON. After intervention N300 amplitudes of IMP were comparable to those of CON and NIMP. This suggests that the N300, which has been related to phonological access of orthographic stimuli and integration of orthographic and phonological representations, might index a compensatory mechanism or precursor that facilitates reading improvement. The N400, which is thought to reflect grapheme-phoneme conversion or the access to the orthographic lexicon increased in IMP from pre to post and was comparable to CON after intervention. Correlations between N300 amplitudes pre, growth in reading ability and N400 amplitudes post indicated that higher N300 amplitudes might be important for reading improvement and increase in N400 amplitudes. The results suggest that children with DD, showing the same cognitive profile might differ regarding their neuronal profile which could further influence reading improvement. PMID:25018723

JWST Pathfinder Telescope Risk Reduction Cryo Test Program

NASA Technical Reports Server (NTRS)

Matthews, Gary W.; Scorse, Thomas R.; Spina, John A.; Noel, Darin M.; Havey, Keith A., Jr.; Huguet, Jesse A.; Whitman, Tony L.; Wells, Conrad; Walker, Chanda B.; Lunt, Sharon;

MESUR Pathfinder Science Investigations

NASA Technical Reports Server (NTRS)

Golombek, M.

1993-01-01

The MESUR (Mars Environmental Survey) Pathfinder mission is the first Discovery mission planned for launch in 1996. MESUR Pathfinder is designed as an engineering demonstration of the entry, descent and landing approach to be employed by the follow-on MESUR Network mission, which will land of order 10 small stations on the surface of Mars to investigate interior, atmospheric and surface properties. Pathfinder is a small Mars lander, equipped with a microrover to deploy instruments and explore the local landing site. Instruments selected for Pathfinder include a surface imager on a 1 m pop-up mast (stereo with spectral filters), an atmospheric structure instrument/surface meteorology package, and an alpha proton x-ray spectrometer. The microrover will carry the alpha proton x-ray spectrometer to a number of different rocks and surface materials and provide close-up imaging...

The Infant Motor Profile: a standardized and qualitative method to assess motor behaviour in infancy.

PubMed

Heineman, Kirsten R; Bos, Arend F; Hadders-Algra, Mijna

2008-04-01

A reliable and valid instrument to assess neuromotor condition in infancy is a prerequisite for early detection of developmental motor disorders. We developed a video-based assessment of motor behaviour, the Infant Motor Profile (IMP), to evaluate motor abilities, movement variability, ability to select motor strategies, movement symmetry, and fluency. The IMP consists of 80 items and is applicable in children from 3 to 18 months. The present study aimed to test intra- and interobserver reliability and concurrent validity of the IMP with the Alberta Infant Motor Scale (AIMS) and Touwen neurological examination. The study group consisted of 40 low-risk term (median gestational age [GA] 40 wks, range 38-42 wks) and 40 high-risk preterm infants (median GA 29.6 wks, range 26-33 wks) with corrected ages 4 to 18 months (31 females, 49 males). Intra- and interobserver agreement of the IMP were satisfactory (Spearman's rho=0.9). Concurrent validity of IMP and AIMS was good (Spearman's rho=0.8, p<0.005). The IMP was able to differentiate between infants with normal neurological condition, simple minor neurological dysfunction (MND), complex MND, and abnormal neurological condition (p<0.005). This means that the IMP may be a promising tool to evaluate neurological integrity during infancy, a suggestion that needs confirmation by means of assessment of larger groups of infants with heterogeneous neurological conditions.

Accumulation of I-123 IMP in hepatic cell adenoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suto, Yuji; Kodama, Fumiko; Kato, Takashi

1995-07-01

I-123 IMP is now widely used as a radioactive material for cerebral blood flow scintigraphy. It is also known that this substance will accumulate in certain types of tumors. The authors present a case of a 47-year-old woman who showed accumulation of I-123 IMP in hepatic cell adenoma. 6 refs., 3 figs.

Dissemination of genetically related IMP-6-producing multidrug-resistant Pseudomonas aeruginosa ST235 in South Korea.

PubMed

Yoo, Jung Sik; Yang, Ji Woo; Kim, Hye Mee; Byeon, Jeongheum; Kim, Hwa Su; Yoo, Jae Il; Chung, Gyung Tae; Lee, Yeong Seon

2012-04-01

The present study aimed to describe the prevalence and molecular epidemiology of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa isolates obtained from non-tertiary care hospitals and geriatric hospitals in South Korea. Of the 644 isolates, 224 were carbapenem-resistant, amongst which 41 (18.3%) were MBL-producers and the major MBL type was IMP-6 (35 isolates). IMP-6-producing isolates were multidrug-resistant and showed higher minimum inhibitory concentrations for meropenem than imipenem. All of the IMP-6-producing isolates had class 1 integrons with amplification sizes of 4.5 kb/5.5 kb (34 isolates) or 3.0 kb (1 isolate); 4.5 kb/5.5 kb integrons had bla(IMP-6)-qac-aacA4-bla(OXA-1)-aadA1 (5.5 kb) and aadB-cmlA-bla(OXA-10)-aadA1 (4.5 kb). Pulsed-field gel electrophoresis (PFGE) analysis indicated that all IMP-6-producing P. aeruginosa from various geographic areas had nearly identical patterns with >85% similarity. All IMP-6-producing isolates showed high genetic similarity to those obtained from tertiary care hospitals and had the same integron type, indicating the spread of these strains to the three types of hospitals nationwide. These data show the wide spreading of clonally related IMP-6-producing P. aeruginosa (sequence type 235) through tertiary, non-tertiary and geriatric hospitals in South Korea. Continuous monitoring and thorough infection control should be performed in all types of hospitals to prevent further spreading of MBL-producing P. aeruginosa. Copyright © 2012 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

Exquisite Modulation of the Active Site of Methanocaldococcus jannaschii Adenylosuccinate Synthetase in Forward Reaction Complexes.

PubMed

Karnawat, Vishakha; Mehrotra, Sonali; Balaram, Hemalatha; Puranik, Mrinalini

2016-05-03

In enzymes that conduct complex reactions involving several substrates and chemical transformations, the active site must reorganize at each step to complement the transition state of that chemical step. Adenylosuccinate synthetase (ADSS) utilizes a molecule each of guanosine 5'-monophosphate (GTP) and aspartate to convert inosine 5'-monophosphate (IMP) into succinyl adenosine 5'-monophosphate (sAMP) through several kinetic intermediates. Here we followed catalysis by ADSS through high-resolution vibrational spectral fingerprints of each substrate and intermediate involved in the forward reaction. Vibrational spectra show differential ligand distortion at each step of catalysis, and band positions of substrates are influenced by binding of cosubstrates. We found that the bound IMP is distorted toward its N1-deprotonated form even in the absence of any other ligands. Several specific interactions between GTP and active-site amino acid residues result in large Raman shifts and contribute substantially to intrinsic binding energy. When both IMP and GTP are simultaneously bound to ADSS, IMP is converted into an intermediate 6-phosphoryl inosine 5'-monophosphate (6-pIMP). The 6-pIMP·ADSS complex was found to be stable upon binding of the third ligand, hadacidin (HDA), an analogue of l-aspartate. We find that in the absence of HDA, 6-pIMP is quickly released from ADSS, is unstable in solution, and converts back into IMP. HDA allosterically stabilizes ADSS through local conformational rearrangements. We captured this complex and determined the spectra and structure of 6-pIMP in its enzyme-bound state. These results provide important insights into the exquisite tuning of active-site interactions with changing substrate at each kinetic step of catalysis.

An injury mortality prediction based on the anatomic injury scale

PubMed Central

Wang, Muding; Wu, Dan; Qiu, Wusi; Wang, Weimi; Zeng, Yunji; Shen, Yi

2017-01-01

Abstract To determine whether the injury mortality prediction (IMP) statistically outperforms the trauma mortality prediction model (TMPM) as a predictor of mortality. The TMPM is currently the best trauma score method, which is based on the anatomic injury. Its ability of mortality prediction is superior to the injury severity score (ISS) and to the new injury severity score (NISS). However, despite its statistical significance, the predictive power of TMPM needs to be further improved. Retrospective cohort study is based on the data of 1,148,359 injured patients in the National Trauma Data Bank hospitalized from 2010 to 2011. Sixty percent of the data was used to derive an empiric measure of severity of different Abbreviated Injury Scale predot codes by taking the weighted average death probabilities of trauma patients. Twenty percent of the data was used to create computing method of the IMP model. The remaining 20% of the data was used to evaluate the statistical performance of IMP and then be compared with the TMPM and the single worst injury by examining area under the receiver operating characteristic curve (ROC), the Hosmer–Lemeshow (HL) statistic, and the Akaike information criterion. IMP exhibits significantly both better discrimination (ROC-IMP, 0.903 [0.899–0.907] and ROC-TMPM, 0.890 [0.886–0.895]) and calibration (HL-IMP, 9.9 [4.4–14.7] and HL-TMPM, 197 [143–248]) compared with TMPM. All models show slight changes after the extension of age, gender, and mechanism of injury, but the extended IMP still dominated TMPM in every performance. The IMP has slight improvement in discrimination and calibration compared with the TMPM and can accurately predict mortality. Therefore, we consider it as a new feasible scoring method in trauma research. PMID:28858124

Animal-vegetal polarity in the plasma membrane of a molluscan egg: a quantitative freeze-fracture study.

PubMed

Speksnijder, J E; Mulder, M M; Dohmen, M R; Hage, W J; Bluemink, J G

1985-03-01

Using freeze-fracture electron microscopy, the numerical particle distribution in the fertilized Nassarius egg plasma membrane has been analyzed in four areas at different positions along the animal-vegetal axis of the egg. These areas can be distinguished by distinct microvilli patterns and differences in microvilli densities. In all areas, more IMPs (intramembrane particles) are present on the P face than on the corresponding E face. The ratio of the number of IMPs present on E and P face is similar in all areas (0.48-0.55) except for the most animal part of the vegetal hemisphere, where relatively more IMPs remain attached to the exterior half of the fractured membrane (E/P ratio = 0.88). The IMP density at the vegetal pole of the egg is considerably higher than in the animal hemisphere and in the animal part of the vegetal hemisphere. This difference is due to an increased number of IMPs in all size classes (4-18 nm). In the area adjacent to the vegetal pole the density of particles is also higher than in the two more animal areas, but here the difference is exclusively due to the smaller IMP size classes (4-8 nm). Statistical analysis of our data reveals that the area adjacent to the vegetal pole patch is significantly different from the other areas with respect to the distribution of the IMPs over the different IMP size classes. These results demonstrate the polar organization of the Nassarius egg plasma membrane. The possible role of this surface heterogeneity in the spatial organization of the egg cell and the later embryo is discussed.

Visualization of RNA–protein interactions in living cells: FMRP and IMP1 interact on mRNAs

PubMed Central

Rackham, Oliver; Brown, Chris M

2004-01-01

Protein expression depends significantly on the stability, translation efficiency and localization of mRNA. These qualities are largely dictated by the RNA-binding proteins associated with an mRNA. Here, we report a method to visualize and localize RNA–protein interactions in living mammalian cells. Using this method, we found that the fragile X mental retardation protein (FMRP) isoform 18 and the human zipcode-binding protein 1 ortholog IMP1, an RNA transport factor, were present on common mRNAs. These interactions occurred predominantly in the cytoplasm, in granular structures. In addition, FMRP and IMP1 interacted independently of RNA. Tethering of FMRP to an mRNA caused IMP1 to be recruited to the same mRNA and resulted in granule formation. The intimate association of FMRP and IMP1 suggests a link between mRNA transport and translational repression in mammalian cells. PMID:15282548

Reduction and Analysis of Meteorology Data from the Mars Pathfinder Lander

NASA Technical Reports Server (NTRS)

Murphy, James R.; Bridger, Alison F. C.; Haberle, Robert M.

1998-01-01

Dr. James Murphy is a member of the Mars Pathfinder Atmospheric Structure Investigation Meteorology (ASI/MET) Science Team. The activities of Dr. Murphy, and his collaborators are summarized in this report, which reviews the activities in support of the analysis of the meteorology data from the Mars Pathfinder Lander.

AVHRR-Based Polar Pathfinder Products: Evaluation, Enhancement, and Transition to MODIS

NASA Technical Reports Server (NTRS)

Fowler, Charles; Maslanik, James; Stone, Robert; Stroeve, Julienne; Emery, William

2001-01-01

The AVHRR-Based Polar Pathfinder (APP) products include calibrated AVHRR channel data, surface temperatures, albedo, satellite scan and solar geometries, and a cloud mask composited into twice- per-day images, and daily averaged fields of sea ice motion, for regions poleward of 50 deg. latitude. Our goals under this grant, in general, are four-fold: 1. To quantify the APP accuracy and sources of error by comparing Pathfinder products with field measurements. 2. To determine the consistency of mean fields and trends in comparison with longer time series of available station data and forecast model output. 3. To investigate the consistency of the products between the different AVHRR instruments over the 1982-present period of the NOAA program. 4. To compare an annual cycle of the AVHRR Pathfinder products with MODIS to establish a baseline for extending Pathfinder-type products into the new ESE period. Year One tasks include intercomparisons of the Pathfinder products with field measurements, testing of algorithm assumptions, collection of field data, and further validation and possible improvement of the multi-sensor ice motion fields. Achievements for these tasks are summarized below.

MARS PATHFINDER PYRO SYSTEMS SWITCHING ACTIVITY

NASA Technical Reports Server (NTRS)

1996-01-01

The Mars Pathfinder lander is subjected to a electrical and functional tests of its pyrotechic petal deployer system by Jet Propulsion Laboratory (JPL) engineers and technicians in KSC's Spacecraft Assembly and Encapsulation Facility (SAEF-2). In the background is the Pathfinder cruise stage, which the lander will be mated to once its functional tests are complete. The lander will remain attached to this stage during its six-to-seven-month journey to Mars. When the lander touches down on the surface of Mars next year, the pyrotechnic system will deploy its three petals open like a flower and allow the Sojourner autonomous rover to explore the Martian surface. The Mars Pathfinder is scheduled for launch aboard a Delta II expendable launch vehicle on Dec. 2, the beginning of a 24-day launch period. JPL is managing the Mars Pathfinder project for NASA.

IMp: The customizable LEGO(®) Pinned Insect Manipulator.

PubMed

Dupont, Steen; Price, Benjamin; Blagoderov, Vladimir

2015-01-01

We present a pinned insect manipulator (IMp) constructed of LEGO® building bricks with two axes of movement and two axes of rotation. In addition we present three variants of the IMp to emphasise the modular design, which facilitates resizing to meet the full range of pinned insect specimens, is fully customizable, collapsible, affordable and does not require specialist tools or knowledge to assemble.

Construct Validity of the Infant Motor Profile: Relation with Prenatal, Perinatal, and Neonatal Risk Factors

ERIC Educational Resources Information Center

Heineman, Kirsten R.; La Bastide-Van Gemert, Sacha; Fidler, Vaclav; Middelburg, Karin J.; Bos, Arend F.; Hadders-Algra, Mijna

2010-01-01

Aim: The Infant Motor Profile (IMP) is a qualitative assessment of motor behaviour of infants aged 3 to 18 months. The aim of this study was to investigate construct validity of the IMP through the relation of IMP scores with prenatal, perinatal, and neonatal variables, including the presence of brain pathology indicated by neonatal ultrasound…

Incorporation of a recombinant Eimeria maxima IMP1 antigen into nanoparticles confers protective immunity against E. maxima challenge infection

USDA-ARS?s Scientific Manuscript database

The purpose of this study was to determine if incorporating a recombinant Eimeria maxima protein, namely rEmaxIMP1, into gold nanoparticles (NP) could improve the level of protective immunity against E. maxima challenge infection. Recombinant EmaxIMP1 was expressed in Escherchia coli as a poly-His f...

IMP: Using microsat technology to support engineering research inside of the International Space Station

NASA Astrophysics Data System (ADS)

Carroll, Kieran A.

2000-01-01

This paper describes an International Space Station (ISS) experiment-support facility being developed by Dynacon for the Canadian Space Agency (CSA), based on microsatellite technology. The facility is called the ``Intravehicular Maneuverable Platform,'' or IMP. The core of IMP is a small, free-floating platform (or ``bus'') deployed inside one of the pressurized crew modules of ISS. Exchangeable experimental payloads can then be mounted to the IMP bus, in order to carry out engineering development or demonstration tests, or microgravity science experiments: the bus provides these payloads with services typical of a standard satellite bus (power, attitude control, etc.). The IMP facility takes advantage of unique features of the ISS, such as the Shuttle-based logistics system and the continuous availability of crew members, to greatly reduce the expense of carrying out space engineering experiments. Further cost reduction has been made possible by incorporating technology that Dynacon has developed for use in a current microsatellite mission. Numerous potential payloads for IMP have been identified, and the first of these (a flexible satellite control experiment) is under development by Dynacon and the University of Toronto's Institute for Aerospace Studies, for the CSA. .

A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties

PubMed Central

Nishino, Jinsuke; Kim, Sunjung; Zhu, Yuan; Zhu, Hao; Morrison, Sean J

2013-01-01

Stem cell properties change over time to match the changing growth and regeneration demands of tissues. We showed previously that adult forebrain stem cell function declines during aging because of increased expression of let-7 microRNAs, evolutionarily conserved heterochronic genes that reduce HMGA2 expression. Here we asked whether let-7 targets also regulate changes between fetal and adult stem cells. We found a second let-7 target, the RNA binding protein IMP1, that is expressed by fetal, but not adult, neural stem cells. IMP1 expression was promoted by Wnt signaling and Lin28a expression and opposed by let-7 microRNAs. Imp1-deficient neural stem cells were prematurely depleted in the dorsal telencephalon due to accelerated differentiation, impairing pallial expansion. IMP1 post-transcriptionally inhibited the expression of differentiation-associated genes while promoting the expression of self-renewal genes, including Hmga2. A network of heterochronic gene products including Lin28a, let-7, IMP1, and HMGA2 thus regulates temporal changes in stem cell properties. DOI: http://dx.doi.org/10.7554/eLife.00924.001 PMID:24192035

Impaired expression of importin/karyopherin {beta}1 leads to post-implantation lethality

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miura, Katsutaka; Yoshinobu, Kumiko; Imaizumi, Takashi

2006-03-03

Importin {beta}1 (Imp{beta})/karyopherin {beta}1 (Kpnb1) mediates the nuclear import of a large variety of substrates. This study aimed to investigate the requirement for the Kpnb1 gene in mouse development, using a gene trap line, B6-CB-Ayu8108 {sup GtgeoIMEG} (Ayu8108 {sup geo}), in which the trap vector was inserted into the promoter region of the Kpnb1 gene, but in reverse orientation of the Kpnb1 gene. Ayu8108 {sup geo/geo} homozygous embryos could develop to the blastocyst stage, but died before embryonic day 5.5, and expression of the Kpnb1 gene in homozygous blastocysts was undetectable. We also replaced the {beta}geo gene with Imp{beta} cDNAmore » through Cre-mediated recombination to rescue Imp{beta} expression. Homozygous mice for the rescued allele Ayu8108 {sup Imp{beta}}{sup /Imp{beta}} were born and developed normally. These results demonstrated that the cause of post-implantation lethality of Ayu8108 {sup geo/geo} homozygous embryos was impaired expression of the Kpnb1 gene, indicating indispensable roles of Imp{beta}1 in early development of mice.« less

Relative to the Caltech Electron/Isotope Spectrometer-IMP

NASA Technical Reports Server (NTRS)

1997-01-01

The Electron Isotope Spectrometers (EIS) on IMP-8 was designed to provide measurements of low energy isotopes from H to O (1 less than or equal to Z less than or equal to 8) over the energy range from approx. 2 to approx. 40 MeV/nuc, as well as low energy electrons from approx. 0.2 to approx. 6 MeV. These measurements supported studies of the acceleration and transport of energetic particles originating on the Sun, in interplanetary space, in the Galaxy, and in Earth's and Jupiter's magnetosphere. IMP-8 was launched in October 1973 and still continues to provide key particles and fields measurements from orbit about the Earth. The IMP-8 EIS continued to operate flawlessly until 8/3/92, when a partial failure was suffered in a bias supply, which reduced the ability of the instrument to measure high energy particles. The EIS was subsequently turned off in 1995. A nearly identical instrument was flown on IMP-7 from 10/92 to 7/96, and many studies combined results from both instruments. For completeness, this report summarizes scientific results and publications from the entire IMP-7&8 effort, not just from the period covered by NAG5-727.

Luteal phase bleeding after IVF cycles: comparison between progesterone vaginal gel and intramuscular progesterone and correlation with pregnancy outcomes

PubMed Central

Jabara, Sami; Barnhart, Kurt; Schertz, Joan C; Patrizio, Pasquale

2009-01-01

Background: To compare luteal phase bleeding and pregnancy outcomes in normogonadotropic patients receiving progesterone vaginal gel (PVG) or intramuscular progesterone (IMP) injections. Methods: In this retrospective cohort study, data from 270 patients (292 cycles) undergoing day-3 fresh embryo transfer were analyzed. PVG, 90 mg daily (170 cycles) or IMP, 50 mg daily (122 cycles) began at egg retrieval. Results: Luteal phase bleeding was significantly more common in the PVG than the IMP group. No significant differences were observed in biochemical pregnancy or spontaneous abortion rates between the two groups. Patients who bled before the pregnancy test had significantly lower total and clinical pregnancy rates than non-bleeders. Total and ongoing pregnancy/delivery rates were higher in the PVG than IMP group, but did not achieve statistical significance. Conclusion: Luteal phase bleeding was more common in the PVG group than the IMP group, but pregnancy was successful in more patients in the PVG group. Luteal phase bleeding is prevented or delayed during IMP treatment, but patients who bled before the pregnancy test, whether using the gel or injected progesterone, had significantly reduced pregnancy rates compared with non-bleeders. PMID:20485581

IMP3 Stabilization of WNT5B mRNA Facilitates TAZ Activation in Breast Cancer.

PubMed

Samanta, Sanjoy; Guru, Santosh; Elaimy, Ameer L; Amante, John J; Ou, Jianhong; Yu, Jun; Zhu, Lihua J; Mercurio, Arthur M

2018-05-29

Insulin-like growth factor-2 mRNA-binding protein 3 (IMP3) is an oncofetal protein associated with many aggressive cancers and implicated in the function of breast cancer stem cells (CSCs). The mechanisms involved, however, are poorly understood. We observed that IMP3 facilitates the activation of TAZ, a transcriptional co-activator of Hippo signaling that is necessary for the function of breast CSCs. The mechanism by which IMP3 activates TAZ involves both mRNA stability and transcriptional regulation. IMP3 stabilizes the mRNA of an alternative WNT ligand (WNT5B) indirectly by repressing miR145-5p, which targets WNT5B, resulting in TAZ activation by alternative WNT signaling. IMP3 also facilitates the transcription of SLUG, which is necessary for TAZ nuclear localization and activation, by a mechanism that is also mediated by WNT5B. These results demonstrate that TAZ can be regulated by an mRNA-binding protein and that this regulation involves the integration of Hippo and alternative WNT-signaling pathways. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

A review of parameters and heuristics for guiding metabolic pathfinding.

PubMed

Kim, Sarah M; Peña, Matthew I; Moll, Mark; Bennett, George N; Kavraki, Lydia E

2017-09-15

Recent developments in metabolic engineering have led to the successful biosynthesis of valuable products, such as the precursor of the antimalarial compound, artemisinin, and opioid precursor, thebaine. Synthesizing these traditionally plant-derived compounds in genetically modified yeast cells introduces the possibility of significantly reducing the total time and resources required for their production, and in turn, allows these valuable compounds to become cheaper and more readily available. Most biosynthesis pathways used in metabolic engineering applications have been discovered manually, requiring a tedious search of existing literature and metabolic databases. However, the recent rapid development of available metabolic information has enabled the development of automated approaches for identifying novel pathways. Computer-assisted pathfinding has the potential to save biochemists time in the initial discovery steps of metabolic engineering. In this paper, we review the parameters and heuristics used to guide the search in recent pathfinding algorithms. These parameters and heuristics capture information on the metabolic network structure, compound structures, reaction features, and organism-specificity of pathways. No one metabolic pathfinding algorithm or search parameter stands out as the best to use broadly for solving the pathfinding problem, as each method and parameter has its own strengths and shortcomings. As assisted pathfinding approaches continue to become more sophisticated, the development of better methods for visualizing pathway results and integrating these results into existing metabolic engineering practices is also important for encouraging wider use of these pathfinding methods.

Validation of the Version 1 NOAA/NASA Pathfinder Sea Surface Temperature Data Set

NASA Technical Reports Server (NTRS)

Smith, Elizabeth A.

1998-01-01

A high-resolution, global satellite-derived sea surface temperature (SST) data set called Pathfinder, from the Advanced Very High Resolution Radiometer (AVHRR) aboard the NOAA Polar Orbiters, is available from the Jet Propulsion Laboratory Physical Oceanography Distributed Active Archive Center (JPL PO.DAAC). Suitable for research as well as education, the Pathfinder SST data set is a result of a collaboration between the National Oceanographic and Atmospheric Administration (NOAA), the National Aeronautics and Space Administration (NASA) and investigators at several universities. NOAA and NASA are the sponsors of the Pathfinder Program, which takes advantage of currently archived Earth science data from satellites. Where necessary, satellite sensors have been intercalibrated, algorithms improved and processing procedures revised, in order to produce long time-series, global measurements of ocean, land and atmospheric properties necessary for climate research. Many Pathfinder data sets are available to researchers now, nearly a decade before the first launch of NASA's Earth Observing System (EOS). The lessons learned from the Pathfinder programs will facilitate the processing and management of terabytes of data from EOS. The Oceans component of Pathfinder has undertaken to reprocess all Global Area Coverage (GAC) data acquired by the 5-channel AVHRRs since 1981. The resultant data products are consistent and stably calibrated [Rao, 1993a, Rao, 1993b, Brown et al., 1993], Earth-gridded SST fields at a variety of spatial and temporal resolutions.

Wind and IMP 8 Solar Wind, Magnetosheath and Shock Data

NASA Technical Reports Server (NTRS)

2004-01-01

The purpose of this project was to provide the community access to magnetosheath data near Earth. We provided 27 years of IMP 8 magnetosheath proton velocities, densities, and temperatures with our best (usually 1-min.) time resolution. IMP 8 crosses the magnetosheath twice each 125 day orbit, and we provided magnetosheath data for the roughly 27 years of data for which magnetometer data are also available (which are needed to reliably pick boundaries). We provided this 27 years of IMP 8 magnetosheath data to the NSSDC; this data is now integrated with the IMP 8 solar wind data with flags indicating whether each data point is in the solar wind, magnetosheath, or at the boundary between the two regions. The plasma speed, density, and temperature are provided for each magnetosheath point. These data are also available on the MIT web site ftp://space .mit.edu/pub/plasma/imp/www/imp.html. We provide ASCII time-ordered rows of data giving the observation time, the spacecraft position in GSE, the velocity is GSE, the density and temperature for protons. We also have analyzed and archived on our web site the Wind magnetosheath plasma parameters. These consist of ascii files of the proton and alpha densities, speeds, and thermal speeds. These data are available at ftp://space.mit.edu/pub/plasma/wind/sheath These are the two products promised in the work statement and they have been completed in full.

Empirical therapies among adults hospitalized for community-acquired upper urinary tract infections: A decision-tree analysis of mortality, costs, and resistance.

PubMed

Parienti, J J; Lucet, J C; Lefort, A; Armand-Lefèvre, L; Wolff, M; Caron, F; Cattoir, V; Yazdanpanah, Y

2015-09-01

Poor outcomes occur when patients with serious infections receive antibiotics to which the organisms are resistant. Decision trees simulated in-hospital mortality, costs, incremental cost-effectiveness ratio per life year saved, and carbapenem resistance according to 3 empirical antibiotic strategies among adults hospitalized for community-acquired (CA) upper urinary tract infections (UTIs): ceftriaxone (CRO) plus gentamicin (GM) in the intensive care unit (ICU), imipenem (IMP), and individualized choice (IMP or CRO) based on clinical risk factors for CA- extended-spectrum β-lactamase (ESBL). The estimated prevalence of CA-ESBL on admission was 5% (range, 1.3%-17.6%); 3% and 97% were admitted to the ICU and medical ward (MW), respectively. In the ICU, CRO plus GM was dominated; IMP was cost-effective (incremental cost-effectiveness ratio: €4,400 per life year saved compared with individualized choice). In the MW, IMP had no impact on mortality and was less costly (-€142 per patient vs CRO, -€38 vs individualized choice). The dominance of IMP was consistent in sensitivity analyses. Compared with CRO, colonization by carbapenem-resistant pathogens increased by an odds ratio of 4.5 in the IMP strategy. Among the ICU patients, empirical IMP therapy reduces mortality at an acceptable cost. Among MW patients, individualized choice or CRO is preferred to limit carbapenem resistance at a reasonable cost. Copyright © 2015 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

An outer membrane protein (OmpA) of Escherichia coli K-12 undergoes a conformational change during export.

PubMed

Freudl, R; Schwarz, H; Stierhof, Y D; Gamon, K; Hindennach, I; Henning, U

1986-08-25

Pulse-chase experiments were performed to follow the export of the Escherichia coli outer membrane protein OmpA. Besides the pro-OmpA protein, which carries a 21-residue signal sequence, three species of ompA gene products were distinguishable. One probably represented an incomplete nascent chain, another the mature protein in the outer membrane, and the third, designated imp-OmpA (immature processed), a protein which was already processed but apparently was still associated with the plasma membrane. The pro- and imp-OmpA proteins could be characterized more fully by using a strain overproducing the ompA gene products; pro- and imp-OmpA accumulated in large amounts. It could be shown that the imp- and pro-OmpA proteins differ markedly in conformation from the OmpA protein. The imp-OmpA, but not the pro-OmpA, underwent a conformational change and gained phage receptor activity upon addition of lipopolysaccharide. Utilizing a difference in detergent solubility between the two polypeptides and employing immunoelectron microscopy, it could be demonstrated that the pro-OmpA protein accumulated in the cytoplasm while the imp-OmpA was present in the periplasmic space. The results suggest that the pro-OmpA protein, bound to the plasma membrane, is processed, and the resulting imp-OmpA, still associated with the plasma membrane, recognizes the lipid A moiety of the lipopolysaccharide. The resulting conformational change may then force the protein into the outer membrane.

GeneImp: Fast Imputation to Large Reference Panels Using Genotype Likelihoods from Ultralow Coverage Sequencing

PubMed Central

Spiliopoulou, Athina; Colombo, Marco; Orchard, Peter; Agakov, Felix; McKeigue, Paul

2017-01-01

We address the task of genotype imputation to a dense reference panel given genotype likelihoods computed from ultralow coverage sequencing as inputs. In this setting, the data have a high-level of missingness or uncertainty, and are thus more amenable to a probabilistic representation. Most existing imputation algorithms are not well suited for this situation, as they rely on prephasing for computational efficiency, and, without definite genotype calls, the prephasing task becomes computationally expensive. We describe GeneImp, a program for genotype imputation that does not require prephasing and is computationally tractable for whole-genome imputation. GeneImp does not explicitly model recombination, instead it capitalizes on the existence of large reference panels—comprising thousands of reference haplotypes—and assumes that the reference haplotypes can adequately represent the target haplotypes over short regions unaltered. We validate GeneImp based on data from ultralow coverage sequencing (0.5×), and compare its performance to the most recent version of BEAGLE that can perform this task. We show that GeneImp achieves imputation quality very close to that of BEAGLE, using one to two orders of magnitude less time, without an increase in memory complexity. Therefore, GeneImp is the first practical choice for whole-genome imputation to a dense reference panel when prephasing cannot be applied, for instance, in datasets produced via ultralow coverage sequencing. A related future application for GeneImp is whole-genome imputation based on the off-target reads from deep whole-exome sequencing. PMID:28348060

Pathfinder Instruments for Cloud and Aerosol Spaceborne Observations (PICASSO)

NASA Technical Reports Server (NTRS)

McCormick, M. Patrick; Winker, David M.

1998-01-01

This paper will describe the planned 3-year Pathfinder Instruments for Cloud and Aerosol Spaceborne Observations (PICASSO) mission, its instrumentation and implementation. It will use LITE and other data, plus analyses, to show the feasibility of such a mission. PICASSO is being proposed for NASA's Earth System Science Pathfinder (ESSP) program with launch predicted in 2003.

Pathfinder-Plus flight in Hawaii

NASA Technical Reports Server (NTRS)

2002-01-01

Pathfinder-Plus flight in Hawaii June 2002 AeroVironment's Pathfinder-Plus solar-powered flying wing recently flew a three-flight demonstration of its ability to relay third-generation cell phone and video signals as well as provide Internet linkage. The two pods underneath the center section of the wing carried the advanced two-way telecom package, developed by Japanese telecommunications interests.

Looking Back in Time: Building the James Webb Space Telescope (JWST) Optical Telescope Element

NASA Technical Reports Server (NTRS)

Feinberg, Lee

2016-01-01

When it launches in 2018, the James Webb Space Telescope (JWST) will look back in time at the earliest stars and galaxies forming in the universe. This talk will look back in time at the development of the JWST telescope. This will include a discussion of the design, technology development, mirror development, wave front sensing and control algorithms, lightweight cryogenic deployable structure, pathfinder telescope, and integration and test program evolution and status. The talk will provide the engineering answers on why the mirrors are made of Beryllium, why there are 18 segments, where and how the mirrors were made, how the mirrors get aligned using the main science camera, and how the telescope is being tested. It will also look back in time at the many dedicated people all over the country who helped build it.

The Mars Pathfinder Mission and Science Results

NASA Technical Reports Server (NTRS)

Golombek, M. P.

1999-01-01

Mars Pathfinder, the first low-cost, quick Discovery class mission to be completed, successfully landed on the surface of Mars on July 4, 1997, deployed and navigated a small rover, and collected data from 3 science instruments and 10 technology experiments. The mission operated on Mars for 3 months and returned 2.3 Gbits of new data, including over 16,500 lander and 550 rover images, 16 chemical analyses of rocks and soil, and 8.5 million individual temperature, pressure and wind measurements. The rover traversed 100 m clockwise around the lander, exploring about 200 square meters of the surface. The mission captured the imagination of the public, and garnered front page headlines during the first week. A total of about 566 million internet "hits" were registered during the first month of the mission, with 47 million "hits" on July 8th alone, making the Pathfinder landing by far the largest internet event in history at the time. Pathfinder was the first mission to deploy a rover on Mars. It carried a chemical analysis instrument, to characterize the rocks and soils in a landing area over hundreds of square meters on Mars, which provided a calibration point or "ground truth" for orbital remote sensing observations. The combination of spectral imaging of the landing area by the lander camera, chemical analyses aboard the rover, and close-up imaging of colors, textures and fabrics with the rover cameras offered the potential of identifying rocks (petrology and mineralogy). With this payload, a landing site in Ares Vallis was selected because it appeared acceptably safe and offered the prospect of analyzing a variety of rock types expected to be deposited by catastrophic floods, which enabled addressing first-order scientific questions such as differentiation of the crust, the development of weathering products, and the nature of the early Martian environment and its subsequent evolution. The 3 instruments and rover allowed seven areas of scientific investigation: the geology and geomorphology of the surface, mineralogy and geochemistry of rocks and soils, physical properties of surface materials, magnetic properties of airborne dust, atmospheric science including aerosols, and rotational and orbital dynamics of Mars. Scientists were assembled into 7 Science Operations Groups that were responsible for requesting measurements by the 3 instruments, rover and engineering subsystems for carrying out their scientific investigations and for analyzing the data and reporting on their findings. The spacecraft was launched on December 4, 1996 and had a 7 month cruise to Mars, with four trajectory correction maneuvers. The vehicle entered the atmosphere directly following cruise stage separation. Parachute deployment, heatshield and lander separation, radar ground acquisition, airbag inflation and rocket ignition all occurred before landing at 2:58 AM true local solar time (9:56:55 AM PDT). The lander bounced at least 15 times up to 12 in high without airbag rupture, demonstrating the robustness of this landing system. Reconstruction of the final landing sequence indicates that the parachute/backshel1/1ander was tilted due to a northwest directed wind and wind shear, which resulted in the lander bouncing about I km to the northwest and initially downhill about 20 m from where the solid rockets fired. Two anomalously bright spots located in the lander scene are likely the heatshield, which continued in a ballistic trajectory about 2 km downrange (west southwest), and the backshell/parachute, which stayed nearer to where the rockets fired. Unconnected disturbed soil patches in the scene indicate that the final few bounces of the lander were from the east-southeast and were followed by a gentle roll to the west before coming to rest on the base petal. The location of the lander away from where the solid rockets fired and considerations of the exhaust products used to inflate the airbags and their fate, indicate that the Pathfinder landing system is one of the cleanest designed leaving the local area essentially contaminant free. The radio signal from the low-=gain antenna was received at 11:34 AM PDT indicating a successful landing.

Gambling on the Protestants: the Pathfinder Fund and birth control in Peru, 1958-1965.

PubMed

López, L Necochea

2014-01-01

Among the agencies involved in population control activities in the mid-twentieth century, none scored as many early victories in Latin America as did the Pathfinder Fund, founded by Procter & Gamble scion Clarence Gamble. This article analyzes a style in the delivery of family planning assistance in the developing world through the work of the Pathfinder Fund in Peru, the organization's hub in South America, and shows how Pathfinder personnel collaborated with local Protestant institutions. Its Protestant allies helped Pathfinder set up and manage rapid interventions such as the production of pamphlets, the smuggling of contraceptives, and the enrollment of physicians as advocates of the use of intrauterine devices. Although these rapid interventions helped quickly disseminate information and certain technologies among a fortunate few, they also weakened legitimate state agencies, neglected the monitoring of the safety of the drugs supplied, and alienated allies with their high-handed boldness.

LISA Pathfinder: An important first step towards a space-based gravitational wave observatory

NASA Astrophysics Data System (ADS)

Thorpe, James

2017-08-01

ESA's LISA Pathfinder mission was launched on Dec 3rd, 2015 and completed earlier this Summer. During this relatively short mission, Pathfinder at its two science payloads, Europe's LISA Technology Package and NASA's Disturbance Reduction System, demonstrated several techniques and technologies that enable development of a future space-based gravitational wave observatory. Most notably, Pathfinder demonstrated that the technique of drag-free flight could be utilized to place a test mass in near-perfect free-fall, with residual accelerations at the femto-g level in the milliHertz band. Additionally, technologies such as precision bonded optical structures for metrology, micropropulsion systems, and non-contact charge control, were successfully tested, retiring risk for LISA. In this talk, I will present an overview of Pathfinder's results to date and some perspective on how this success will be leveraged into realizing LISA.

IMp: The customizable LEGO® Pinned Insect Manipulator

PubMed Central

Dupont, Steen; Price, Benjamin; Blagoderov, Vladimir

2015-01-01

Abstract We present a pinned insect manipulator (IMp) constructed of LEGO® building bricks with two axes of movement and two axes of rotation. In addition we present three variants of the IMp to emphasise the modular design, which facilitates resizing to meet the full range of pinned insect specimens, is fully customizable, collapsible, affordable and does not require specialist tools or knowledge to assemble. PMID:25685035

Dynamic hydraulic fluid stimulation regulated intramedullary pressure.

PubMed

Hu, Minyi; Serra-Hsu, Frederick; Bethel, Neville; Lin, Liangjun; Ferreri, Suzanne; Cheng, Jiqi; Qin, Yi-Xian

2013-11-01

Physical signals within the bone, i.e. generated from mechanical loading, have the potential to initiate skeletal adaptation. Strong evidence has pointed to bone fluid flow (BFF) as a media between an external load and the bone cells, in which altered velocity and pressure can ultimately initiate the mechanotransduction and the remodeling process within the bone. Load-induced BFF can be altered by factors such as intramedullary pressure (ImP) and/or bone matrix strain, mediating bone adaptation. Previous studies have shown that BFF induced by ImP alone, with minimum bone strain, can initiate bone remodeling. However, identifying induced ImP dynamics and bone strain factor in vivo using a non-invasive method still remains challenging. To apply ImP as a means for alteration of BFF, it was hypothesized that non-invasive dynamic hydraulic stimulation (DHS) can induce local ImP with minimal bone strain to potentially elicit osteogenic adaptive responses via bone-muscle coupling. The goal of this study was to evaluate the immediate effects on local and distant ImP and strain in response to a range of loading frequencies using DHS. Simultaneous femoral and tibial ImP and bone strain values were measured in three 15-month-old female Sprague Dawley rats during DHS loading on the tibia with frequencies of 1Hz to 10Hz. DHS showed noticeable effects on ImP induction in the stimulated tibia in a nonlinear fashion in response to DHS over the range of loading frequencies, where they peaked at 2Hz. DHS at various loading frequencies generated minimal bone strain in the tibiae. Maximal bone strain measured at all loading frequencies was less than 8με. No detectable induction of ImP or bone strain was observed in the femur. This study suggested that oscillatory DHS may regulate the local fluid dynamics with minimal mechanical strain in the bone, which serves critically in bone adaptation. These results clearly implied DHS's potential as an effective, non-invasive intervention for osteopenia and osteoporosis treatments. © 2013. Published by Elsevier Inc. All rights reserved.

Young Lunar Volcanic Features: How Did They Form?

NASA Astrophysics Data System (ADS)

Elder, Catherine; Hayne, Paul O.; Donaldson Hanna, Kerri; Bandfield, Joshua; Ghent, Rebecca; Williams, Jean-Pierre; Paige, David A.

2016-10-01

Irregular mare patches (IMPs) on the Moon are younger than 100 Myr [1, 2], but their formation mechanism is unknown. Previous work has suggested caldera collapse [3], explosive outgassing [2], pyroclastic eruptions [4], basaltic lava flows [1, 5], and regolith drainage into graben [6]. Here we present observations from the Lunar Reconnaissance Orbiter (LRO) Diviner thermal radiometer of the four largest IMPs. These observations suggest that the surfaces of the IMPs are on average only slightly rockier than the surrounding regolith. The nighttime cooling curves of the IMPs and the surrounding regolith do not intersect, which suggests that there is no layering in the top 5-10 cm of the IMPs. We also measure the thermal inertia (parameterized through the "H-parameter" [7]) of the IMPs. We find that the thermal inertia of Sosigenes is higher than that of the surrounding regolith (probably due to mass wasting), the thermal inertia of Cauchy-5 and Maskelyne is not significantly different from the surrounding regolith, and the thermal inertia of the largest smooth mound in Ina is significantly lower than the surrounding regolith. Only some IMPs are in topographic depressions or associated with graben, so neither caldera collapse nor drainage into graben can explain the formation of all IMPs. It is unlikely that basaltic lava flows would lead to a thermal inertia lower than that of lunar regolith. Therefore, of the formation mechanisms proposed to date, pyroclastic eruptions or another type of explosive outgassing [e.g. 2] possibly accompanied by basaltic lava flows or drainage into graben best explain the available observations of IMPs.[1] Braden, S. et al. (2014) Nature Geo 7, 787-791. [2] Schultz, P. H. et al. (2006) Nature 444, 184-186. [3] El-Baz, F. (1973) Apollo 17: Preliminary Science Report 330, 30-13. [4] Carter, L. B. et al. (2013) LPSC 44, 2146. [5] Garry, W. B. et al. (2012) JGR 117, E00H31. [6] Qiao, L. et al. (2002) LPSC 47, 2002. [7] Vasavada, A. R. et al. (2012) JGR 117, E00H18.Part of this work was performed at the Jet Propulsion Laboratory, California Institute of Technology under contract with the National Aeronautics and Space Administration.

Pathfinder aircraft flight #1

NASA Image and Video Library

1996-11-19

The Pathfinder research aircraft's solar cell arrays are prominently displayed as it touches down on the bed of Rogers Dry Lake at the Dryden Flight Research Center, Edwards, California, following a test flight. The solar arrays covered more than 75 percent of Pathfinder's upper wing surface, and provided electricity to power its six electric motors, flight controls, communications links and a host of scientific sensors.

Mars Pathfinder Landing Site Workshop

NASA Technical Reports Server (NTRS)

Golombek, Matthew (Editor)

1994-01-01

The Mars Pathfinder Project is an approved Discovery-class mission that will place a lander and rover on the surface of the Red Planet in July 1997. The Mars Pathfinder Landing Site Workshop was designed to allow the Mars scientific community to provide input as to where to land Pathfinder on Mars. The workshop was attended by over 60 people from around the United States and from Europe. Over 20 landing sites were proposed at the workshop, and the scientific questions and problems concerning each were addressed. The workshop and the discussion that occured during and afterward have significantly improved the ability to select a scientifically exciting but safe landing site on Mars.

Pathfinder aircraft flight #1

NASA Image and Video Library

1996-11-19

The Pathfinder solar-powered research aircraft settles in for landing on the bed of Rogers Dry Lake at the Dryden Flight Research Center, Edwards, California, after a successful test flight Nov. 19, 1996. The ultra-light craft flew a racetrack pattern at low altitudes over the flight test area for two hours while project engineers checked out various systems and sensors on the uninhabited aircraft. The Pathfinder was controlled by two pilots, one in a mobile control unit which followed the craft, the other in a stationary control station. Pathfinder, developed by AeroVironment, Inc., is one of several designs being evaluated under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program.

Oligonucleotide synthesis catalyzed by the Zn/2+/ ion

NASA Technical Reports Server (NTRS)

Sawai, H.; Orgel, L. E.

1975-01-01

Results of experiments are reported in which Zn(2+) ion catalyzed the formation of oligonucleotides from nucleoside phosphorimidazolides in aqueous solution, even in the absence of a template. Specifically, the imidazolides (ImpU or ImpA) polymerized to form ImpApA, and pApA, pApApA, and pApApApA, or the analogous uracil compounds. In addition, the expected hydrolysis products of the hydrolysis of ImpA were formed (pA, imidazole). Judging from the ratio of pA(n) over pA (with and without zinc ion), this ion increased the efficiency of phosphodiester-bond formation by up to 10 times. Possible mechanisms for the reaction are tentatively proposed.

Visible and Near-Infrared Properties of Optical Fibers Coupled to the Pathfinder High-Resolution NIR Spectrograph

NASA Astrophysics Data System (ADS)

McCoy, K.; Ramsey, L.

2011-09-01

The Penn State Astronomy and Astrophysics Department’s Pathfinder instrument is a fiber-fed, warm-bench echelle spectrograph designed to explore technical issues that must be resolved in order to measure precise radial velocities that will allow the detection of exoplanets in the near-infrared (NIR). In May 2010, Pathfinder demonstrated 10-20 m/s radial-velocity precision in the NIR at the 9 meter Hobby-Eberly Telescope. To attain even higher precision, we are investigating the NIR properties of the optical fibers that transmit light from the telescope to Pathfinder. We conducted a series of modal noise tests with visible and NIR laser diodes on a 200 micron diameter, fused-silica, multimode optical fiber as the preliminary step in analyzing the degrading effects of modal noise on radial-velocity precision. We report these test results and comment on our future tests to reduce the negative effects of modal noise and focal ratio degradation (FRD). The lessons learned from this research and the Pathfinder prototype will be used in Pathfinder II, which will aim to achieve better than 5 m/s in the NIR.

Hemimegalencephaly: Clinical, EEG, neuroimaging, and IMP-SPECT correlation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Konkol, R.J.; Maister, B.H.; Wells, R.G.

1990-11-01

Iofetamine-single photon emission computed tomography (IMP-SPECT) was performed on 2 girls (5 1/2 and 6 years of age) with histories of intractable seizures, developmental delay, and unilateral hemiparesis secondary to hemimegalencephaly. Electroencephalography (EEG) revealed frequent focal discharges in 1 patient, while a nearly continuous burst suppression pattern over the malformed hemisphere was recorded in the other. IMP-SPECT demonstrated a good correlation with neuroimaging studies. In spite of the different EEG patterns, which had been proposed to predict contrasting clinical outcomes, both IMP-SPECT scans disclosed a similar decrease in tracer uptake in the malformed hemisphere. These results are consistent with themore » pattern of decreased tracer uptake found in other interictal studies of focal seizures without cerebral malformations. In view of recent recommendations for hemispherectomy in these patients, we suggest that the IMP-SPECT scan be used to compliment EEG as a method to define the extent of abnormality which may be more relevant to long-term prognosis than EEG alone.« less

Interface Message Processors for the ARPA Computer Network

DTIC Science & Technology

1975-04-01

Pluribus IMP construction and checkout; sizeable changes to the i*4P message-processing algorithms: and Satellite IMP issues. The IMP message...extremely low cost modification design. We have begun to consider changes to the MLC design which would enable the MLC to suppress continuous breaks...existing authentication mechanisms need not make these changes . 2.7 Other Topics During the first quarter BBN constructed an environmental test chamber

IDC Integrated Master Plan.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clifford, David J.; Harris, James M.

2014-12-01

This is the IDC Re-Engineering Phase 2 project Integrated Master Plan (IMP). The IMP presents the major accomplishments planned over time to re-engineer the IDC system. The IMP and the associate Integrated Master Schedule (IMS) are used for planning, scheduling, executing, and tracking the project technical work efforts. REVISIONS Version Date Author/Team Revision Description Authorized by V1.0 12/2014 IDC Re- engineering Project Team Initial delivery M. Harris

Distinct Signaling Roles of cIMP, cCMP, and cUMP.

PubMed

Seifert, Roland

2016-10-04

The cyclic purine nucleotide cIMP and the cyclic pyrimidine nucleotides cCMP and cUMP are emerging second messengers. These cNMPs show different biological effects, but the molecular mechanisms remain elusive. In this issue of Structure, Ng et al. (2016) provide structural evidence for distinct interactions of cIMP, cCMP, and cUMP with ion channels. Copyright © 2016 Elsevier Ltd. All rights reserved.

IMP-1 and a Novel Metallo-β-Lactamase, VIM-6, in Fluorescent Pseudomonads Isolated in Singapore

PubMed Central

Koh, Tse Hsien; Wang, Grace Chee Yeng; Sng, Li-Hwei

2004-01-01

Four carbapenem-resistant Pseudomonas spp. were isolated from patients in Singapore. One Pseudomonas putida isolate contained a blaIMP-1 identical to that first described in Japan. The sequence of a variant blaIMP-1 in Pseudomonas fluorescens contained four silent mutations compared with the original sequence. The remaining P. putida isolates contained blaVIM-6, a novel VIM gene variant. PMID:15155248

[Simultaneous determination of sivelestat and its metabolite XW-IMP-A in human plasma using HPLC-MS/MS].

PubMed

Wang, Jing; Dai, Xiao-jian; Zhang, Yi-fan; Zhong, Da-fang; Wu, Yu-lin; Chen, Xiao-yan

2015-10-01

A simple and rapid method was developed based on high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to determine sivelestat and its metabolite XW-IMP-A in human plasma. After a simple protein precipitation, the samples and internal standards were analyzed on a C18 column by a gradient elution program. The mobile phase consisted of 30% acetonitrile in methanol and 5 mmol · L(-1) ammonium acetate at a flow rate of 0.7 mL · min(-1). The mass spectrometric data was collected in multiple reaction monitoring mode (MRM) in the negative electrospray ionization. The standard curves were linear in the range of 10.0-15,000 ng · mL(-1) for sivelestat, and 2.50-1000 ng · mL(-1) for XW-IMP-A. The low limits of quantitation were identified at 10.0 and 2.50 ng · mL for sivelestat and XW-IMP-A, respectively. The intra- and inter-day precision were within 11.3% and 13.1% for sivelestat and XW-IMP-A, and accuracy was 0.3% and 0.6% for sivelestat and XW-IMP-A, within the acceptable limits across all concentrations. The method was successfully validated in the pharmacokinetic study of sivelestat in healthy Chinese volunteers.

Mechanics of the fast-start: muscle function and the role of intramuscular pressure in the escape behavior of amia calva and polypterus palmas

PubMed

Westneat; Hale; Mchenry; Long

1998-11-01

The fast-start escape response is a rapid, powerful body motion used to generate high accelerations of the body in virtually all fishes. Although the neurobiology and behavior of the fast-start are often studied, the patterns of muscle activity and muscle force production during escape are less well understood. We studied the fast-starts of two basal actinopterygian fishes (Amia calva and Polypterus palmas) to investigate the functional morphology of the fast-start and the role of intramuscular pressure (IMP) in escape behavior. Our goals were to determine whether IMP increases during fast starts, to look for associations between muscle activity and elevated IMP, and to determine the functional role of IMP in the mechanics of the escape response. We simultaneously recorded the kinematics, muscle activity patterns and IMP of four A. calva and three P. palmas during the escape response. Both species generated high IMPs of up to 90 kPa (nearly 1 atmosphere) above ambient during the fast-start. The two species showed similar pressure magnitudes but had significantly different motor patterns and escape performance. Stage 1 of the fast-start was generated by simultaneous contraction of locomotor muscle on both sides of the body, although electromyogram amplitudes on the contralateral (convex) side of the fish were significantly lower than on the ipsilateral (concave) side. Simultaneous recordings of IMP, escape motion and muscle activity suggest that pressure change is caused by the contraction and radial swelling of cone-shaped myomeres. We develop a model of IMP production that incorporates myomere geometry, the concept of constant-volume muscular hydrostats, the relationship between fiber angle and muscle force, and the forces that muscle fibers produce. The timing profile of pressure change, behavior and muscle action indicates that elevated muscle pressure is a mechanism of stiffening the body and functions in force transmission during the escape response.

The Impact of Cognitive Impairment in Dementia on Self-Care Domains in Diabetes Mellitus: A Systematic Search and Narrative Review.

PubMed

Santos, Tamsin; Lovell, Janaka; Shiell, Kerrie; Johnson, Marilyn; Ibrahim, Joseph E

2018-04-29

Self-management is integral to effective chronic disease management. Cognitive impairments (CogImp) associated with dementia have not previously been reviewed in diabetes mellitus (DM) self-care. (i) Whether CogImp associated with dementia impact self-care. (ii) Whether specific CogImp affects key DM self-care processes. A systematic literature search with a narrative review was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. This review examined studies published from January, 2000 to February, 2016 describing the relationship between cognition and DM self-care domains in community dwelling older adults with dementia/CogImp. Eight studies met inclusion criteria. Decrements in all self-care domains were associated with CogImp. Problem solving was related to reduced disease knowledge (OR 0.87, 95% CI=0.49-1.55), resulting in poorer glycemic control. Decision-making impairments manifested as difficulties in adjusting insulin doses, leading to more hospital admissions. People without CogImp were better able to find/utilize resources by adhering to recommended management (OR 1.03, 95% CI=1.02-1.05). A lack of interaction with health care providers was demonstrated through reduced receipt of important routine investigation including eye examinations (ARR=0.85, 95% CI=0.85-0.86), HbA1c testing (ARR=0.96, 95% CI=0.96-0.97) and LDL-C testing (ARR=0.91, 95% CI=0.901-0.914). People without CogImp had better clinic attendance (OR 2.17, 95% CI=1.30-3.70). Action taking deficits were apparent through less self-testing of blood sugar levels (20.2% vs 24.4%, p=0.1) resulting in poorer glycemic control, self-care and more frequent micro/macrovascular complications. Persons with diabetes and CogImp, particularly in domains of learning, memory and executive function, were significantly impaired in all self-care tasks. This article is protected by copyright. All rights reserved.

Identification, synthesis and structural characterization of process related and degradation impurities of acrivastine and validation of HPLC method.

PubMed

Kumar, Ajay; Devineni, Subba Rao; Dubey, Shailender Kumar; Kumar, Pradeep; Srivastava, Vishal; Ambulgekar, Girish; Jain, Mohit; Gupta, Dharmendra Kumar; Singh, Gurmeet; Kumar, Rajesh; Hiriyanna, S G; Kumar, Pramod

2016-10-17

Four impurities (Imp-I-IV) were detected using gradient HPLC method in few laboratory batches of acrivastine in the level of 0.03-0.12% and three impurities (Imp-I-III) were found to be known and one (Imp-IV) was unknown. In forced degradation study, the drug is degraded into four degradation products under oxidation and photolytic conditions. Two impurities (Imp-III and -IV) were concurred with process related impurities whereas Imp-V and -VI were identified as new degradation impurities. Based on LC-ESI/MS n study, the chemical structures of new impurities were presumed as 1-[(2E)-3-(4-methylphenyl)-3-{6-[(1E)-3-oxobut-1-en-1-yl]pyridin-2-yl}prop-2-en-1-yl]pyrrolidin-1-ium-1-olate (Imp-IV), 1-{[3-(4-methylphenyl)-3-{6-[(1E)-3-oxobut-1-en-1-yl]pyridin-2-yl}oxiran-2-yl]methyl}pyrrolidin-1-ium-1-olate (Imp-V) and 2-[2-(4-methylphenyl)-3-[(1-oxidopyrrolidin-1-ium-1-yl)methyl]oxiran-2-yl]-6-[(1E)-3-oxobut-1-en-1-yl]pyridin-1-ium-1-olate (Imp-VI), and confirmed by their synthesis followed by spectroscopic analysis, IR, NMR ( 1 H, 13 C) and mass. An efficient and selective high-performance liquid chromatography method has been developed and resolved well the drug related substances on a Phenomenex Gemini C-18 (250×4.6mm, particle size 5μm) column. The mobile phase was composed of sodium dihydrogen phosphate (10mM) and methanol, temperature at 25°C, and a PDA detector set at 254nm used for detection. The method was validated with respect to specificity, linearity, precision, accuracy, and sensitivity and satisfactory results were achieved. Identification, synthesis, characterization of impurities and method validation were first reported in this paper. Copyright © 2016 Elsevier B.V. All rights reserved.

Cytotoxic and pro-oxidative effects of Imperata cylindrica aerial part ethyl acetate extract in colorectal cancer in vitro.

PubMed

Kwok, Amy Ho Yan; Wang, Yan; Ho, Wing Shing

2016-05-15

Colorectal cancer (CRC) is the third most common cancer. Its global incidence and mortality have been on the rise. Recent strategy of therapies has involved the use of non-steroid anti-inflammatory drugs and cyclooxygenase-selective inhibitors. Aerial parts of Imperata cylindrical L. Raeusch (IMP) have been used as an anti-inflammatory agent in traditional Chinese medicine. Asarachidonate acid cascadeis often involved in inflammation-related malignancy and IMP is an anti-inflammatory agent, hence it is hypothesized that IMP aerial part ethyl acetate extract exerts cytotoxic effects on colorectal cancer cells in vitro. The HT-29 adenocarcinoma cell line was used to elucidate its pro-apoptotic activities. Flow cytometry and fluorescent microscopy were performed to assess cell cycle arrest and the accumulation of reactive oxygen species (ROS). The mRNA and hormone levels of arachidonate acid pathways were studied via quantitative reverse transcription PCR (qRT-PCR) and ELISA. The 50% growth inhibitory effect (GI50) of the IMP extract on HT-29 was measured with a value of 14.5 µg/ml. Immuno-blot and caspase-3/7 activity assay showed the pro-apoptotic effect of IMP on the activation of caspase cascade. G2/M arrest was observed via flow cytometry. The ROS activity was modulated by the IMP extraction a concentration-dependent manner in HT-29 cells. The IMP extract increased PGE2 and PGF2α levels qRT-PCR revealed that transcripts of rate-limiting PGE2- and PGF2α-biosynthetic enzymes - COX-1, mPGES1 and AKR1C3 were notably up-regulated. Among the prostanoid receptors, EP1 and FP transcripts were up-regulated while EP4 transcripts decreased. The findings suggest that the proliferative effect of PGE2, which is generally believed to associate with heightened DNA synthesis and cross-talk with MAPK pathways, is likely triggered by the pro-apoptotic or -oxidative effects exerted by IMP extract in HT-29 cells. Concurring with this notion, indomethacin (COX-1/2-inhibitor) was demonstrated to potentiate the cytotoxic effect of IMP extract (GI50 ≦ 10.8 µg/ml). The results show that the cytotoxic effect of IMP extract predominates over the influence of proliferative prostanoids released by challenged colorectal cancer cells, and may present a potential source for development of novel anti-cancer drugs. Copyright © 2016 Elsevier GmbH. All rights reserved.

Motoneuron axon pathfinding errors in zebrafish: Differential effects related to concentration and timing of nicotine exposure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Menelaou, Evdokia; Paul, Latoya T.; Perera, Surangi N.

Nicotine exposure during embryonic stages of development can affect many neurodevelopmental processes. In the developing zebrafish, exposure to nicotine was reported to cause axonal pathfinding errors in the later born secondary motoneurons (SMNs). These alterations in SMN axon morphology coincided with muscle degeneration at high nicotine concentrations (15–30 μM). Previous work showed that the paralytic mutant zebrafish known as sofa potato exhibited nicotine-induced effects onto SMN axons at these high concentrations but in the absence of any muscle deficits, indicating that pathfinding errors could occur independent of muscle effects. In this study, we used varying concentrations of nicotine at differentmore » developmental windows of exposure to specifically isolate its effects onto subpopulations of motoneuron axons. We found that nicotine exposure can affect SMN axon morphology in a dose-dependent manner. At low concentrations of nicotine, SMN axons exhibited pathfinding errors, in the absence of any nicotine-induced muscle abnormalities. Moreover, the nicotine exposure paradigms used affected the 3 subpopulations of SMN axons differently, but the dorsal projecting SMN axons were primarily affected. We then identified morphologically distinct pathfinding errors that best described the nicotine-induced effects on dorsal projecting SMN axons. To test whether SMN pathfinding was potentially influenced by alterations in the early born primary motoneuron (PMN), we performed dual labeling studies, where both PMN and SMN axons were simultaneously labeled with antibodies. We show that only a subset of the SMN axon pathfinding errors coincided with abnormal PMN axonal targeting in nicotine-exposed zebrafish. We conclude that nicotine exposure can exert differential effects depending on the levels of nicotine and developmental exposure window. - Highlights: • Embryonic nicotine exposure can specifically affect secondary motoneuron axons in a dose-dependent manner. • The nicotine-induced secondary motoneuron axonal pathfinding errors can occur independent of any muscle fiber alterations. • Nicotine exposure primarily affects dorsal projecting secondary motoneurons axons. • Nicotine-induced primary motoneuron axon pathfinding errors can influence secondary motoneuron axon morphology.« less

IMP-8. Volume 2: Scientific section. [Bibliography

NASA Technical Reports Server (NTRS)

1980-01-01

Results of the analysis of the IMP-8 data, which was collected during the first six and one-half years after launch of the IMP-8 spacecraft are presented. The plasma wave experiment data were processed and are available in an easily accessible summary form. These data continue to provide a valuable source for comparative studies with plasma wave experiments on other spacecraft operating in the solar wind and within the Earth's magnetosphere.

Emery-Kivelson solution of the two-channel Kondo problem

NASA Astrophysics Data System (ADS)

Sengupta, Anirvan M.; Georges, Antoine

1994-04-01

We consider the two-channel Kondo model in the Emery-Kivelson approach, and calculate the total susceptibility enhancement due to the impurity χimp=χ-χbulk. We find that χimp exactly vanishes at the solvable point, in a completely analogous way to the singular part of the specific heat Cimp. A perturbative calculation around the solvable point yields the generic behavior χimp~log(1/T), Cimp~T logT and the known universal value of the Wilson ratio RW=8/3. From this calculation, the Kondo temperature can be identified and is found to behave as the inverse square of the perturbation parameter. The small-field, zero-temperature behavior χimp~log(1/h) is also recovered.

JWST Pathfinder Telescope Integration

NASA Technical Reports Server (NTRS)

Matthews, Gary W.; Kennard, Scott H.; Broccolo, Ronald T.; Ellis, James M.; Daly, Elizabeth A.; Hahn, Walter G.; Amon, John N.; Mt. Pleasant, Stephen M.; Texter, Scott; Atkinson, Charles B.;

Pathfinder aircraft liftoff on altitude record setting flight of 71,500 feet

NASA Image and Video Library

1997-07-07

The Pathfinder aircraft has set a new unofficial world record for high-altitude flight of over 71,500 feet for solar-powered aircraft at the U.S. Navy's Pacific Missile Range Facility, Kauai, Hawaii. Pathfinder was designed and manufactured by AeroVironment, Inc, of Simi Valley, California, and was operated by the firm under a jointly sponsored research agreement with NASA's Dryden Flight Research Center, Edwards, California. Pathfinder's record-breaking flight occurred July 7, 1997. The aircraft took off at 11:34 a.m. PDT, passed its previous record altitude of 67,350 feet at about 5:45 p.m. and then reached its new record altitude at 7 p.m. The mission ended with a perfect nighttime landing at 2:05 a.m. PDT July 8. The new record is the highest altitude ever attained by a propellor-driven aircraft. Before Pathfinder, the altitude record for propellor-driven aircraft was 67,028 feet, set by the experimental Boeing Condor remotely piloted aircraft.

Morning Martian Atmospheric Temperature Gradients and Fluctuations Observed by Mars Pathfinder

NASA Technical Reports Server (NTRS)

Mihalov, John D.; Haberle, R. M.; Murphy, J. R.; Seiff, A.; Wilson, G. R.

1999-01-01

We have studied the most prominent atmospheric temperature fluctuations observed during Martian mornings by Mars Pathfinder and have concluded, based on comparisons with wind directions, that they appear to be a result of atmospheric heating associated with the Lander spacecraft. Also, we have examined the morning surface layer temperature lapse rates, which are found to decrease as autumn approaches at the Pathfinder location, and which have mean (and median) values as large as 7.3 K/m in the earlier portions of the Pathfinder landed mission. It is plausible that brief isolated periods with gradients twice as steep are associated with atmospheric heating adjacent to Lander air bag material. In addition, we have calculated the gradient with height of the structure function obtained with Mars Pathfinder, for Mars' atmospheric temperatures measured within about 1.3 m from the surface, assuming a power law dependence, and have found that these gradients superficially resemble those reported for the upper region of the terrestrial stable boundary layer.

High Resolution Airborne Digital Imagery for Precision Agriculture

NASA Technical Reports Server (NTRS)

Herwitz, Stanley R.

1998-01-01

The Environmental Research Aircraft and Sensor Technology (ERAST) program is a NASA initiative that seeks to demonstrate the application of cost-effective aircraft and sensor technology to private commercial ventures. In 1997-98, a series of flight-demonstrations and image acquisition efforts were conducted over the Hawaiian Islands using a remotely-piloted solar- powered platform (Pathfinder) and a fixed-wing piloted aircraft (Navajo) equipped with a Kodak DCS450 CIR (color infrared) digital camera. As an ERAST Science Team Member, I defined a set of flight lines over the largest coffee plantation in Hawaii: the Kauai Coffee Company's 4,000 acre Koloa Estate. Past studies have demonstrated the applications of airborne digital imaging to agricultural management. Few studies have examined the usefulness of high resolution airborne multispectral imagery with 10 cm pixel sizes. The Kodak digital camera integrated with ERAST's Airborne Real Time Imaging System (ARTIS) which generated multiband CCD images consisting of 6 x 106 pixel elements. At the designated flight altitude of 1,000 feet over the coffee plantation, pixel size was 10 cm. The study involved the analysis of imagery acquired on 5 March 1998 for the detection of anomalous reflectance values and for the definition of spectral signatures as indicators of tree vigor and treatment effectiveness (e.g., drip irrigation; fertilizer application).

Mars Pathfinder Rover-Lewis Research Center Technology Experiments Program

NASA Technical Reports Server (NTRS)

Stevenson, Steven M.

1997-01-01

An overview of NASA's Mars Pathfinder Program is given and the development and role of three technology experiments from NASA's Lewis Research Center and carried on the Mars Pathfinder rover is described. Two recent missions to Mars were developed and managed by the Jet Propulsion Laboratory, and launched late last year: Mars Global Surveyor in November 1996 and Mars Pathfinder in December 1996. Mars Global Surveyor is an orbiter which will survey the planet with a number of different instruments, and will arrive in September 1997, and Mars Pathfinder which consists of a lander and a small rover, landing on Mars July 4, 1997. These are the first two missions of the Mars Exploration Program consisting of a ten year series of small robotic martian probes to be launched every 26 months. The Pathfinder rover will perform a number of technology and operational experiments which will provide the engineering information necessary to design and operate more complex, scientifically oriented surface missions involving roving vehicles and other machinery operating in the martian environment. Because of its expertise in space power systems and technologies, space mechanisms and tribology, Lewis Research Center was asked by the Jet Propulsion Laboratory, which is heading the Mars Pathfinder Program, to contribute three experiments concerning the effects of the martian environment on surface solar power systems and the abrasive qualities of the Mars surface material. In addition, rover static charging was investigated and a static discharge system of several fine Tungsten points was developed and fixed to the rover. These experiments and current findings are described herein.

High-resolution topomapping of candidate MER landing sites with Mars Orbiter Camera narrow-angle images

USGS Publications Warehouse

Kirk, R.L.; Howington-Kraus, E.; Redding, B.; Galuszka, D.; Hare, T.M.; Archinal, B.A.; Soderblom, L.A.; Barrett, J.M.

2003-01-01

We analyzed narrow-angle Mars Orbiter Camera (MOC-NA) images to produce high-resolution digital elevation models (DEMs) in order to provide topographic and slope information needed to assess the safety of candidate landing sites for the Mars Exploration Rovers (MER) and to assess the accuracy of our results by a variety of tests. The mapping techniques developed also support geoscientific studies and can be used with all present and planned Mars-orbiting scanner cameras. Photogrammetric analysis of MOC stereopairs yields DEMs with 3-pixel (typically 10 m) horizontal resolution, vertical precision consistent with ???0.22 pixel matching errors (typically a few meters), and slope errors of 1-3??. These DEMs are controlled to the Mars Orbiter Laser Altimeter (MOLA) global data set and consistent with it at the limits of resolution. Photoclinometry yields DEMs with single-pixel (typically ???3 m) horizontal resolution and submeter vertical precision. Where the surface albedo is uniform, the dominant error is 10-20% relative uncertainty in the amplitude of topography and slopes after "calibrating" photoclinometry against a stereo DEM to account for the influence of atmospheric haze. We mapped portions of seven candidate MER sites and the Mars Pathfinder site. Safety of the final four sites (Elysium, Gusev, Isidis, and Meridiani) was assessed by mission engineers by simulating landings on our DEMs of "hazard units" mapped in the sites, with results weighted by the probability of landing on those units; summary slope statistics show that most hazard units are smooth, with only small areas of etched terrain in Gusev crater posing a slope hazard.

Limiting concentrations of activated mononucleotides necessary for poly(C)-directed elongation of oligoguanylates

NASA Technical Reports Server (NTRS)

Kanavarioti, A.; Chang, S.; Alberas, D. J.

1990-01-01

Selected imidazolide-activated nucleotides have been subjected to hydrolysis under conditions similar to those that favor their template-directed oligomerization. Rate constants of hydrolysis of the P-N bond in guanosine 5'-monophosphate 2-methylimidazolide (2-MeImpG) and in guanosine 5'-monophosphate imidazolide (ImpG), kh, have been determined in the presence/absence of magnesium ion as a function of temperature and polycytidylate [poly(C)] concentration. Using the rate constant of hydrolysis of 2-MeImpG and the rate constant of elongation, i.e., the reaction of an oligoguanylate with 2-MeImpG in the presence of poly(C) acting as template, the limiting concentration of 2-MeImpG necessary for oligonucleotide elongation to compete with hydrolysis can be calculated. The limiting concentration is defined as the initial concentration of monomer that results in its equal consumption by hydrolysis and by elongation. These limiting concentrations of 2-MeImpG are found to be 1.7 mM at 37 degrees C and 0.36 mM at 1 degrees C. Boundary conditions in the form of limiting concentration of activated nucleotide may be used to evaluate a prebiotic model for chemical synthesis of biopolymers. For instance, the limiting concentration of monomer can be used as a basis of comparison among catalytic, but nonenzymatic, RNA-type systems. We also determined the rate constant of dimerization of 2-MeImpG, k2 = 0.45 +/- 0.06 M-1 h-1 in the absence of poly(C), and 0.45 +/- 0.06 less than or equal to k2 less than or equal to 0.97 +/- 0.13 M-1 h-1 in its presence at 37 degrees C and pH 7.95.(ABSTRACT TRUNCATED AT 250 WORDS).

Antibiotic resistance pattern and evaluation of metallo-beta lactamase genes (VIM and IMP) in Pseudomonas aeruginosa strains producing MBL enzyme, isolated from patients with secondary immunodeficiency

PubMed Central

Shirani, Kiana; Ataei, Behrouz; Roshandel, Fardad

2016-01-01

Background: One of the most common causes of hospital-acquired secondary infections in hospitalized patients is Pseudomonas aeruginosa. The aim of this study is to evaluate the expression of IMP and VIM in Pseudomonas aeruginosa strains (carbapenem resistant and producer MBL enzyme) in patients with secondary immunodeficiency. Materials and Methods: In a cross sectional study, 96 patients with secondary immunodeficiency hospitalized in the Al-Zahra hospital were selected. Carbapenem resistant strains isolated and modified Hodge test was performed in order to confirm the presence of the metallo carbapenemase enzyme. Under the standard conditions they were sent to the central laboratory for investigating nosocomial infection Multiplex PCR. Results: Of 96 samples 28.1% were IMP positive, 5.2% VIM positive and 3.1% both VIM and IMP positive. The prevalence of multidrug resistance in the IMP and/or VIM negative samples was 29%, while all 5 VIM positive samples have had multidrug resistance. Also the prevalence of multi-drug resistance in IMP positive samples were 96.3% and in IMP and VIM positive samples were 100%. According to Fisher’s test, the prevalence of multi-drug resistance based on gene expression has significant difference (P < 0.001). Conclusion: Based on the results of this study it can be concluded that, a significant percentage of patients with secondary immunodeficiency that suffer nosocomial infections with multidrug resistance, especially Pseudomonas aeruginosa, are probably MBL-producing gene positive. Therefore the cause of infection should be considered in the hospital care system to identify their features, the presence of genes involved in the development of multi-drug resistance and antibiotic therapy. PMID:27563634

Functional analysis of sex-determination genes by gene silencing with LNA-DNA gapmers in the silkworm, Bombyx mori.

PubMed

Sakai, Hiroki; Sakaguchi, Honami; Aoki, Fugaku; Suzuki, Masataka G

2015-08-01

The sexual fate of B. mori is determined genetically; ZW, female and ZZ, male. Recently, we successfully identified a strong candidate gene at the top of the sex determination cascade in B. mori. This gene was termed Feminizer (Fem) and revealed to be a source of Fem-piRNA. Further, we found that B. mori doublesex (Bmdsx) splicing was markedly altered to produce the male-type isoform when a Fem-piRNA inhibitor was injected into ZW embryos. Moreover, knockdown of Masculinizer (Masc), a Fem-piRNA target gene, altered to produce the female-type isoform of Bmdsx in male embryos. However, it remains unclear as to whether Masc directly regulates the sex-specific expression of Bmdsx. In previous studies, we determined that the male-specific isoform of the Bombyx homolog of IGF-II mRNA-binding protein (Imp(M)) was involved in the male-specific splicing of Bmdsx. In an attempt to clarify the genetic relationship between Fem, Masc, Imp(M), and Bmdsx, knockdown experiments were performed. Knockdown of Fem shifted into male-type Bmdsx, Imp(M) and Masc in female embryos. Knockdown of Masc led to the production of the female-type Bmdsx and a dramatic reduction in Imp(M) expression in male embryos. Knockdown of Imp(M) shifted Bmdsx splice mode from the male-type into the female-type. Our results suggest that: (1) Fem reduces Masc expression, (2) Masc dramatically induces Imp(M) expression, and (3) Imp(M) shifting Bmdsx splice mode from the female-type into the male-type. Based on these findings, we propose a possible genetic cascade regulating sex determination in B. mori. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: comparison with detergent micelles, bicelles and liposomes.

PubMed

Lyukmanova, E N; Shenkarev, Z O; Khabibullina, N F; Kopeina, G S; Shulepko, M A; Paramonov, A S; Mineev, K S; Tikhonov, R V; Shingarova, L N; Petrovskaya, L E; Dolgikh, D A; Arseniev, A S; Kirpichnikov, M P

2012-03-01

Production of integral membrane proteins (IMPs) in a folded state is a key prerequisite for their functional and structural studies. In cell-free (CF) expression systems membrane mimicking components could be added to the reaction mixture that promotes IMP production in a soluble form. Here lipid-protein nanodiscs (LPNs) of different lipid compositions (DMPC, DMPG, POPC, POPC/DOPG) have been compared with classical membrane mimicking media such as detergent micelles, lipid/detergent bicelles and liposomes by their ability to support CF synthesis of IMPs in a folded and soluble state. Three model membrane proteins of different topology were used: homodimeric transmembrane (TM) domain of human receptor tyrosine kinase ErbB3 (TM-ErbB3, 1TM); voltage-sensing domain of K(+) channel KvAP (VSD, 4TM); and bacteriorhodopsin from Exiguobacterium sibiricum (ESR, 7TM). Structural and/or functional properties of the synthesized proteins were analyzed. LPNs significantly enhanced synthesis of the IMPs in a soluble form regardless of the lipid composition. A partial disintegration of LPNs composed of unsaturated lipids was observed upon co-translational IMP incorporation. Contrary to detergents the nanodiscs resulted in the synthesis of ~80% active ESR and promoted correct folding of the TM-ErbB3. None of the tested membrane mimetics supported CF synthesis of correctly folded VSD, and the protocol of the domain refolding was developed. The use of LPNs appears to be the most promising approach to CF production of IMPs in a folded state. NMR analysis of (15)N-Ile-TM-ErbB3 co-translationally incorporated into LPNs shows the great prospects of this membrane mimetics for structural studies of IMPs produced by CF systems. Copyright © 2011 Elsevier B.V. All rights reserved.

Intramuscular pressure: A better tool than EMG to optimize exercise for long-duration space flight

NASA Technical Reports Server (NTRS)

Hargens, A. R.; Ballard, R. E.; Aratow, M.; Crenshaw, A.; Styf, J.; Kahan, N.; Watenpaugh, D. E.

1992-01-01

A serious problem experienced by astronauts during long-duration space flight is muscle atrophy. In order to develop countermeasures for this problem, a simple method for monitoring in vivo function of specific muscles is needed. Previous studies document that both intramuscular pressure (IMP) and electromyography (EMG) provide quantitative indices of muscle contraction force during isometric exercise. However, at present there are no data available concerning the usefulness of IMP versus EMG during dynamic exercise. Methods: IMP (Myopress catheter) and surface EMG activity were measured continuously and simultaneously in the tibalis anterior (TA) and soleus (SOL) muscles of 9 normal male volunteers (28-54 years). These parameters were recorded during both concentric and eccentric exercises which consisted of plantarflexon and dorsiflexon of the ankle joint. A Lido Active Isokinetic Dynamometer concurrently recorded ankle joint torque and position. Results: Intramuscular pressure correlated linearly with contraction force for both SOL (r exp 2 = 0.037) and TA (R exp 2 = 0.716 and r exp 2 = 0.802, respectively). During eccentric exercises, SOL and TA IMP also correlated linearly with contraction force (r(exp 2) = 0.883 and r(exp 2) = 0.904 respectively), but SOL and TA EMG correlated poorly with force (r(exp 2) = 0.489 and r(exp 2) = 0.702 respectively). Conclusion: IMP measurement provides a better index of muscle contraction force than EMG during concentric and eccentric exercise. IMP reflects intrinsic mechanical properties of individual muscles, such as length tension relationships. Although invasive, IMP provides a more powerful tool and EMG for developing exercise hardware and protocols for astronauts exposed to long-duration space flight.

IMP dehydrogenase. II. Purification and properties of the enzyme from Yoshida sarcoma ascites tumor cells.

PubMed

Okada, M; Shimura, K; Shiraki, H; Nakagawa, H

1983-11-01

The preceding paper showed that IMP dehydrogenase [IMP:NAD+ oxidoreductase, EC 1.2.1.14] tended to form a precipitable complex(es) through ionic and hydrophobic interactions. On the basis of these observations, a method was developed for purification of IMP dehydrogenase from Yoshida sarcoma ascites cells. On SDS-polyacrylamide gel electrophoresis, the purified preparation (1.19 U/mg protein) appeared homogeneous and its minimum molecular weight was estimated to be 68K daltons. Amino acid analyses indicated a subunit molecular weight of 68,042. Molecular sieve chromatography in the presence of 10% (NH4)2SO4 showed that the molecular weight of the native enzyme was 127K daltons. These values indicate that the native enzyme is composed of two identical subunits. However, the purified enzyme gave 4 protein bands on polyacrylamide gel electrophoresis under non-denaturing conditions, and appeared as a single fraction in the vicinity of the void volume on Ultrogel AcA 34 column chromatography at low salt concentration, indicating that its molecular weight exceeded 200K daltons. These findings indicate that the enzyme tends to aggregate owing to its own physicochemical characteristics. The Km values for IMP and NAD were calculated to be 12 and 25 microM, respectively, and the Ki values for XMP, GMP, and AMP to be 109, 130, and 854 microM, respectively. The purified enzyme showed full activity in the presence of K+, and K+ could be partially replaced by Na+. PCMB inactivated the enzyme, but the activity was completely restored by the addition of DTT. Cl-IMP also inactivated the enzyme and IMP prevented this inactivation.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibiotic resistance pattern and evaluation of metallo-beta lactamase genes (VIM and IMP) in Pseudomonas aeruginosa strains producing MBL enzyme, isolated from patients with secondary immunodeficiency.

PubMed

Shirani, Kiana; Ataei, Behrouz; Roshandel, Fardad

2016-01-01

One of the most common causes of hospital-acquired secondary infections in hospitalized patients is Pseudomonas aeruginosa. The aim of this study is to evaluate the expression of IMP and VIM in Pseudomonas aeruginosa strains (carbapenem resistant and producer MBL enzyme) in patients with secondary immunodeficiency. In a cross sectional study, 96 patients with secondary immunodeficiency hospitalized in the Al-Zahra hospital were selected. Carbapenem resistant strains isolated and modified Hodge test was performed in order to confirm the presence of the metallo carbapenemase enzyme. Under the standard conditions they were sent to the central laboratory for investigating nosocomial infection Multiplex PCR. Of 96 samples 28.1% were IMP positive, 5.2% VIM positive and 3.1% both VIM and IMP positive. The prevalence of multidrug resistance in the IMP and/or VIM negative samples was 29%, while all 5 VIM positive samples have had multidrug resistance. Also the prevalence of multi-drug resistance in IMP positive samples were 96.3% and in IMP and VIM positive samples were 100%. According to Fisher's test, the prevalence of multi-drug resistance based on gene expression has significant difference (P < 0.001). Based on the results of this study it can be concluded that, a significant percentage of patients with secondary immunodeficiency that suffer nosocomial infections with multidrug resistance, especially Pseudomonas aeruginosa, are probably MBL-producing gene positive. Therefore the cause of infection should be considered in the hospital care system to identify their features, the presence of genes involved in the development of multi-drug resistance and antibiotic therapy.

Aerothermal Protuberance Heating Design and Test Configurations for Ascent Vehicle Design

NASA Technical Reports Server (NTRS)

Martin, Charles E.; Neumann, Richard D.; Freeman, Delma

2010-01-01

A series of tests were conducted to evaluate protuberance heating for the purposes of vehicle design and modification. These tests represent a state of the art approach to both testing and instrumentation for defining aerothermal protuberance effects on the protuberance and surrounding areas. The testing was performed with a number of wind tunnel entries beginning with the proof of concept "pathfinder" test in the Test Section 1 (TS1) tunnel in the Langley Unitary Plan Wind Tunnel (UPWT). The TS1 section (see Figures 1a and 1b) is a lower Mach number tunnel and the Test Section 2 (TS2) has overlapping and higher Mach number capability as showin in Figure 1c. The pathfinder concept was proven and testing proceeded for a series of protuberance tests using an existing splitter aluminum protuberance mounting plate, Macor protuberances, thin film gages, total temperature and pressure gages, Kulite pressure transducers, Infra-Red camera imaging, LASER velocimetry evaluations and the UPWT data collection system. A boundary layer rake was used to identify the boundary layer profile at the protuberance locations for testing and helped protuberance design. This paper discusses the techniques and instrumentation used during the protuberance heating tests performed in the UPWT in TS1 and TS2. Runs of the protuberances were made Mach numbers of 1.5, 2.16, 2.65, and 3.51. The data set generated from this testing is for ascent protuberance effects and is termed Protuberance Heating Ascent Data (PHAD) and this testing may be termed PHAD-1 to distinguish it from future testing of this type.

Global Observations of Aerosols and Clouds from Combined Lidar and Passive Instruments to Improve Radiation Budget and Climate Studies

NASA Technical Reports Server (NTRS)

Winker, David M.

1999-01-01

Current uncertainties in the effects of clouds and aerosols on the Earth radiation budget limit our understanding of the climate system and the potential for global climate change. Pathfinder Instruments for Cloud and Aerosol Spaceborne Observations - Climatologie Etendue des Nuages et des Aerosols (PICASSO-CENA) is a recently approved satellite mission within NASA's Earth System Science Pathfinder (ESSP) program which will address these uncertainties with a unique suite of active and passive instruments. The Lidar In-space Technology Experiment (LITE) demonstrated the potential benefits of space lidar for studies of clouds and aerosols. PICASSO-CENA builds on this experience with a payload consisting of a two-wavelength polarization-sensitive lidar, an oxygen A-band spectrometer (ABS), an imaging infrared radiometer (IIR), and a wide field camera (WFC). Data from these instruments will be used to measure the vertical distributions of aerosols and clouds in the atmosphere, as well as optical and physical properties of aerosols and clouds which influence the Earth radiation budget. PICASSO-CENA will be flown in formation with the PM satellite of the NASA Earth Observing System (EOS) to provide a comprehensive suite of coincident measurements of atmospheric state, aerosol and cloud optical properties, and radiative fluxes. The mission will address critical uncertainties iin the direct radiative forcing of aerosols and clouds as well as aerosol influences on cloud radiative properties and cloud-climate radiation feedbacks. PICASSO-CENA is planned for a three year mission, with a launch in early 2003. PICASSO-CENA is being developed within the framework of a collaboration between NASA and CNES.

Interactions Measurement Payload for Shuttle (IMPS) Definition Phase Study.

DTIC Science & Technology

1984-12-15

7 -AS5 222 INTERACTIONS MEASUREMENT PAYLOAD FOR SHUTTLE (IMPS) 1/3 DEFINITION PHASE STUDY(U) JET PROPULSION LAB PASADENA CA G C HILL 15 DEC 84 JPL-D...OF FUNDING NOS. PROGRAM PROJECT TASK WORK UNIT ELEMENT NO. NO NO. NO. S 11 TITLE fnciude Security Classficalion Interactions Measure 63410F 1822 01...block number, d tor Shuttle The Interactions Measurement Payload for hyttle (IMPS) project will study interactions between large space vehicles, such as

Integrated Micro-Power System (IMPS) Development at NASA Glenn Research Center

NASA Technical Reports Server (NTRS)

Wilt, David; Hepp, Aloysius; Moran, Matt; Jenkins, Phillip; Scheiman, David; Raffaelle, Ryne

2003-01-01

Glenn Research Center (GRC) has a long history of energy related technology developments for large space related power systems, including photovoltaics, thermo-mechanical energy conversion, electrochemical energy storage. mechanical energy storage, power management and distribution and power system design. Recently, many of these technologies have begun to be adapted for small, distributed power system applications or Integrated Micro-Power Systems (IMPS). This paper will describe the IMPS component and system demonstration efforts to date.

Evaluation of Etest MBL for Detection of blaIMP-1 and blaVIM-2 Allele-Positive Clinical Isolates of Pseudomonas spp. and Acinetobacter spp.

PubMed Central

Lee, Kyungwon; Yong, Dongeun; Yum, Jong Hwa; Lim, Yong Sik; Bolmström, Anne; Qwärnström, Anette; Karlsson, Åsa; Chong, Yunsop

2005-01-01

The Etest MBL (AB BIODISK, Solna, Sweden) correctly differentiated all 57 isolates of Acinetobacter spp. and Pseudomonas aeruginosa with the blaIMP-1 allele and 135 of 137 (98.5%) Acinetobacter spp. and Pseudomonas spp. isolates with the blaVIM-2 allele. The Etest MBL was reliable for detecting the IMP-1- and VIM-2-producing Pseudomonas and Acinetobacter isolates. PMID:15695713

Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens.

PubMed

Yamamoto, Norihisa; Kawahara, Ryuji; Akeda, Yukihiro; Shanmugakani, Rathina Kumar; Yoshida, Hisao; Hagiya, Hideharu; Hara, Naohiro; Nishi, Isao; Yukawa, Satomi; Asada, Rumiko; Sasaki, Yumi; Maeda, Kazuhiro; Sakamoto, Noriko; Hamada, Shigeyuki; Tomono, Kazunori

2017-03-24

Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. Fifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO 4 ) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO 4 ). A total of 1035 clinical samples were then examined to detect CPE using chromID CARBA and M-ECC medium. All tested strains producing NDM-, KPC-, and OXA-48-carbapenemases were successfully cultured in the media employed. Although most of the IMP-positive strains did not grow in CHROMagar KPC, chromID CARBA, or MC-ECC, all tested strains grew on M-ECC. When faecal samples were applied to the media, M-ECC medium allowed the best growth of IMP-type CPE with a significantly higher sensitivity (99.3%) than that of chromID CARBA (13.9%). M-ECC medium was determined as the most favourable selective medium for the detection of IMP-type CPE as well as other types of CPE.

Inosine-5'-monophosphate is a candidate agent to resolve rigor mortis of skeletal muscle.

PubMed

Matsuishi, Masanori; Tsuji, Mariko; Yamaguchi, Megumi; Kitamura, Natsumi; Tanaka, Sachi; Nakamura, Yukinobu; Okitani, Akihiro

2016-11-01

The object of the present study was to reveal the action of inosine-5'-monophosphate (IMP) toward myofibrils in postmortem muscles. IMP solubilized isolated actomyosin within a narrow range of KCl concentration, 0.19-0.20 mol/L, because of the dissociation of actomyosin into actin and myosin, but it did not solubilize the proteins in myofibrils with 0.2 mol/L KCl. However, IMP could solubilize both proteins in myofibrils with 0.2 mol/L KCl in the presence of 1 m mol/L pyrophosphate or 1.0-3.3 m mol/L adenosine-5'-diphosphate (ADP). Thus, we presumed that pyrophosphate and ADP released thin filaments composed of actin, and thick filaments composed of myosin from restraints of myofibrils, and then both filaments were solubilized through the IMP-induced dissociation of actomyosin. Thus, we concluded that IMP is a candidate agent to resolve rigor mortis because of its ability to break the association between thick and thin filaments. © 2016 Japanese Society of Animal Science.

Detection of VIM- and IMP-type Metallo-Beta-Lactamase Genes in Acinetobacter baumannii Isolates from Patients in Two Hospitals in Tehran

PubMed Central

Davoodi, Saba; Boroumand, Mohammad Ali; Sepehriseresht, Saeed; Pourgholi, Leila

2015-01-01

Background Acinetobacter baumannii, is an opportunistic pathogen and is responsible for numerous nosocomial infections. In recent years, this microorganism has been resistant to a wide range of antibiotics. One of the most important mechanisms of resistance in this microorganism is production of metallo-beta-lactamases (MBLs). Objectives The aim of this study was to detect VIM- and IMP-type metallo-beta-lactamase genes in Acinetobacter baumanniiisolates from patients in two Hospitals in Tehran. Materials and Methods 104 isolates were tested using the PCR method for the identification of VIM- and IMP-type genes. Results vim1, vim2, imp1 and imp2 genes were detected in 6.7%, 41.7%, 50% and 1.7% of the isolates from Tehran Heart Center, and in 29.5%, 38.6%, 4.5% and 4.5% of the isolates from Shahid Mutahhari Hospital respectively. Discussions Our analysis revealed that the majority of the isolates had at least one of these genes, indicating that MBLs production is an important resistance mechanism in Acinetobacter baumannii. PMID:28959283

Telescope Array Control System Based on Wireless Touch Screen Platform

NASA Astrophysics Data System (ADS)

Fu, X. N.; Huang, L.; Wei, J. Y.

2016-07-01

GWAC (Ground-based Wide Angle Cameras) are the ground-based observational instruments of the Sino-French cooperation SVOM (Space Variable Objects Monitor) astronomical satellite, and Mini-GWAC is a pathfinder and supplement of GWAC. In the context of the Mini-GWAC telescope array, this paper introduces the design and implementation of a kind of telescope array control system, which is based on wireless serial interface module to communicate. We describe the development and implementation of the system in detail in terms of control system principle, system hardware structure, software design, experiment, and test. The system uses the touch-control PC which is based on the Windows CE system as the upper-computer, the wireless transceiver module and PLC (Programmable Logic Controller) as the core. It has the advantages of low cost, reliable data transmission, and simple operation. So far, the control system has been applied to Mini-GWAC successfully.

Telescope Array Control System Based on Wireless Touch Screen Platform

NASA Astrophysics Data System (ADS)

Fu, Xia-nan; Huang, Lei; Wei, Jian-yan

2017-10-01

Ground-based Wide Angle Cameras (GMAC) are the ground-based observational facility for the SVOM (Space Variable Object Monitor) astronomical satellite of Sino-French cooperation, and Mini-GWAC is the pathfinder and supplement of GWAC. In the context of the Mini-GWAC telescope array, this paper introduces the design and implementation of a kind of telescope array control system based on the wireless touch screen platform. We describe the development and implementation of the system in detail in terms of control system principle, system hardware structure, software design, experiment, and test etc. The system uses a touch-control PC which is based on the Windows CE system as the upper computer, while the wireless transceiver module and PLC (Programmable Logic Controller) are taken as the system kernel. It has the advantages of low cost, reliable data transmission, and simple operation. And the control system has been applied to the Mini-GWAC successfully.

Teaching, Learning, and Planetary Exploration

NASA Technical Reports Server (NTRS)

Brown, Robert A.

2002-01-01

This is the final report of a program that examined the fundamentals of education associated with space activities, promoted educational policy development in appropriate forums, and developed pathfinder products and services to demonstrate the utility of advanced communication technologies for space-based education. Our focus was on space astrophysics and planetary exploration, with a special emphasis on the themes of the Origins Program, with which the Principal Investigator (PI) had been involved from the outset. Teaching, Learning, and Planetary Exploration was also the core funding of the Space Telescope Science Institute's (ST ScI) Special Studies Office (SSO), and as such had provided basic support for such important NASA studies as the fix for Hubble Space Telescope (HST) spherical aberration, scientific conception of the HST Advanced Camera, specification of the Next-Generation Space Telescope (NGST), and the strategic plan for the second decade of the HST science program.

Stray light suppression in the Goddard IRAM 2-Millimeter Observer (GISMO)

NASA Astrophysics Data System (ADS)

Sharp, E. H.; Benford, D. J.; Fixsen, D. J.; Moseley, S. H.; Staguhn, J. G.; Wollack, E. J.

2012-09-01

The Goddard-IRAM Superconducting 2 Millimeter Observer (GISMO) is an 8x16 Transition Edge Sensor (TES) array of bolometers built as a pathfinder for TES detector development efforts at NASA Goddard Space Flight Center. GISMO has been used annually at the Institut de Radioastronomie Millimétrique (IRAM) 30 meter telescope since 2007 under engineering time and was opened in the spring of 2012 to the general astronomical community. The spring deployment provided an opportunity to modify elements of the room temperature optics before moving the instrument to its new permanent position in the telescope receiver cabin. This allowed for the possibility to extend the cryostat, introduce improved cold baffling and thus further optimize the stray light performance for final astronomical use of the instrument, which has been completed and validated. We will demonstrate and discuss several of the methods used to quantify and limit the influence of stray light in the GISMO camera.

Stray Light Suppression in the Goddard IRAM 2-Millimeter Observer (GISMO)

NASA Technical Reports Server (NTRS)

Sharp, E. H.; Benford, D. J.; Fixsen, D. J.; Moseley, S. H.; Staguhn, J. G.; Wollack, E. J.

2012-01-01

The Goddard-IRAM Superconducting 2 Millimeter Observer (GISMO) is an 8xl6 Transition Edge Sensor (TES) array of bolometers built as a pathfinder for TES detector development efforts at NASA Goddard Space Flight Center. GISMO has been used annually at the Institut de Radioastronomie Millimetrique (IRAM) 30 meter telescope since 2007 under engineering time and was opened in the spring of 2012 to the general astronomical community. The spring deployment provided an opportunity to modify elements of the room temperature optics before moving the instrument to its new permanent position in the telescope receiver cabin. This allowed for the possibility to extend the cryostat, introduce improved cold baffling and thus further optimize the stray light performance for final astronomical use of the instrument, which has been completed and validated. We will demonstrate and discuss several of the methods used to quantify and limit the influence of stray light in the GISMO camera.

Oligomerization reactions of ribonucleotides - The reaction of the 5'-phosphorimidazolide of adenosine with diadenosine pyrophosphate on montmorillonite and other minerals

NASA Technical Reports Server (NTRS)

Ferris, James P.; Ertem, Gozen

1993-01-01

The overall regiospecificity of the 3',5'-phosphodiester bond formation is shown to be increased from 64 to 83 percent when a 9:1 ratio of ImpA to A5'ppA instead of ImpA alone is reacted in the presence of Na(+)-montmorillonite. The major factor in the regiospecificity increase is the formation of 3',5'-linked A5'ppA3'pA and A5'pp-A3'pA3'pA. The results obtained show that ImpA reacts more readily with A5'ppA than it does with another ImpA molecule and that A5'ppA plays a central role in directing the formation of 3',5'-phosphodiester bonds.

Interactions measurement payload for Shuttle

NASA Technical Reports Server (NTRS)

Guidice, D. A.; Pike, C. P.

1985-01-01

The Interactions Measurement Payload for Shuttle (IMPS) consisted of engineering experiments to determine the effects of the space environment on projected Air Force space systems. Measurements by IMPS on a polar-orbit Shuttle flight will lead to detailed knowledge of the interaction of the low-altitude polar-auroral environment on materials, equipment and technologies to be used in future large, high-power space systems. The results from the IMPS measurements will provide direct input to MIL-STD design guidelines and test standards that properly account for space-environment effects.

Pharmacodynamics of Imipenem in Combination with β-Lactamase Inhibitor MK7655 in a Murine Thigh Model

PubMed Central

Mavridou, Eleftheria; Melchers, Ria J. B.; van Mil, Anita C. H. A. M.; Mangin, E.; Motyl, Mary R.

2014-01-01

MK7655 is a newly developed beta-lactamase inhibitor of class A and class C carbapenemases. Pharmacokinetics (PK) of imipenem-cilastatin (IMP/C) and MK7655 were determined for intraperitoneal doses of 4 mg/kg to 128 mg/kg of body weight. MIC and pharmacodynamics (PD) studies of MK7655 were performed against several beta-lactamase producing Pseudomonas aeruginosa and Klebsiella pneumoniae strains to determine its effect in vitro and in vivo. Neutropenic mice were infected in each thigh 2 h before treatment with an inoculum of approximately 5 × 106 CFU. They were treated with IMP/C alone (every 2 hours [q2h], various doses) or in combination with MK7655 in either a dose fractionation study or q2h for 24 h and sacrificed for CFU determinations. IMP/MK7655 decreased MICs regarding IMP MIC. The PK profiles of IMP/C and MK7655 were linear over the dosing range studied and comparable with volumes of distribution (V) of 0.434 and 0.544 liter/kg and half-lives (t1/2) of 0.24 and 0.25 h, respectively. Protein binding of MK7655 was 20%. A sigmoidal maximum effect (Emax) model was fit to the PK/PD index responses. The effect of the inhibitor was not related to the maximum concentration of drug in serum (Cmax)/MIC, and model fits for T>MIC and area under the concentration-time curve (AUC)/MIC were comparable (R2 of 0.7 and 0.75), but there appeared to be no significant relationship of effect with dose frequency. Escalating doses of MK7655 and IMP/C showed that the AUC of MK7655 required for a static effect was dependent on the dose of IMP/C and the MIC of the strain, with a mean area under the concentration-time curve for the free, unbound fraction of the drug (fAUC) of 26.0 mg · h/liter. MK7655 shows significant activity in vivo and results in efficacy of IMP/C in otherwise resistant strains. The exposure-response relationships found can serve as a basis for establishing dosing regimens in humans. PMID:25403667

Motoneuron axon pathfinding errors in zebrafish: Differential effects related to concentration and timing of nicotine exposure

PubMed Central

Menelaou, Evdokia; Paul, Latoya T.; Perera, Surangi N.; Svoboda, Kurt R.

2015-01-01

Nicotine exposure during embryonic stages of development can affect many neurodevelopmental processes. In the developing zebrafish, exposure to nicotine was reported to cause axonal pathfinding errors in the later born secondary motoneurons (SMN). These alterations in SMN axon morphology coincided with muscle degeneration at high nicotine concentrations (15–30µM). Previous work showed that the paralytic mutant zebrafish known as sofa potato, exhibited nicotine-induced effects onto SMN axons at these high concentrations but in the absence of any muscle deficits, indicating that pathfinding errors could occur independent of muscle effects. In this study, we used varying concentrations of nicotine at different developmental windows of exposure to specifically isolate its effects onto subpopulations of motoneuron axons. We found that nicotine exposure can affect SMN axon morphology in a dose-dependent manner. At low concentrations of nicotine, SMN axons exhibited pathfinding errors, in the absence of any nicotine-induced muscle abnormalities. Moreover, the nicotine exposure paradigms used affected the 3 subpopulations of SMN axons differently, but the dorsal projecting SMN axons were primarily affected. We then identified morphologically distinct pathfinding errors that best described the nicotine-induced effects on dorsal projecting SMN axons. To test whether SMN pathfinding was potentially influenced by alterations in the early born primary motoneuron (PMN), we performed dual labeling studies, where both PMN and SMN axons were simultaneously labeled with antibodies. We show that only a subset of the SMN axon pathfinding errors coincided with abnormal PMN axonal targeting in nicotine-exposed zebrafish. We conclude that nicotine exposure can exert differential effects depending on the levels of nicotine and developmental exposure window. PMID:25668718

Pathfinder aircraft taking off - setting new solar powered altitude record

NASA Image and Video Library

1995-09-11

The Pathfinder solar-powered remotely piloted aircraft climbs to a record-setting altitude of 50,567 feet during a flight Sept. 11, 1995, at NASA's Dryden Flight Research Center, Edwards, California. The flight was part of the NASA ERAST (Environmental Research Aircraft and Sensor Technology) program. The Pathfinder was designed and built by AeroVironment Inc., Monrovia, California. Solar arrays cover nearly all of the upper wing surface and produce electricity to power the aircraft's six motors.

MARS PATHFINDER INSPECTED BY ENGINEER LINDA ROBECK IN SAEF-2

NASA Technical Reports Server (NTRS)

1996-01-01

In the SAEF-2 spacecraft checkout facility, engineer Linda Robeck of the Jet Propulsion Laboratory inspects the Mars Pathfinder lander. The spacecraft arrived at Kennedy Space Center from Pasadena, CA on Aug. 13, 1996. The petals of the lander will be opened for checkout of the spacecraft and the installation of the small rover. Launch of Mars Pathfinder aboard a McDonnell Douglas Delta II rocket will occur from Pad B at Complex 17 on Dec. 2.

Pathfinder aircraft flight #1

NASA Image and Video Library

1996-11-19

The Pathfinder solar-powered research aircraft is silhouetted against a clear blue sky as it soars aloft during a checkout flight from the Dryden Flight Research Center, Edwards, California, November, 1996.

Constraints and Approach for Selecting the Mars Surveyor '01 Landing Site

NASA Technical Reports Server (NTRS)

Golombek, M.; Bridges, N.; Gilmore, M.; Haldemann, A.; Parker, T.; Saunders, R.; Spencer, D.; Smith, J.; Weitz, C.

1999-01-01

There are many similarities between the Mars Surveyor '01 (MS '01) landing site selection process and that of Mars Pathfinder. The selection process includes two parallel activities in which engineers define and refine the capabilities of the spacecraft through design, testing and modeling and scientists define a set of landing site constraints based on the spacecraft design and landing scenario. As for Pathfinder, the safety of the site is without question the single most important factor, for the simple reason that failure to land safely yields no science and exposes the mission and program to considerable risk. The selection process must be thorough and defensible and capable of surviving multiple withering reviews similar to the Pathfinder decision. On Pathfinder, this was accomplished by attempting to understand the surface properties of sites using available remote sensing data sets and models based on them. Science objectives are factored into the selection process only after the safety of the site is validated. Finally, as for Pathfinder, the selection process is being done in an open environment with multiple opportunities for community involvement including open workshops, with education and outreach opportunities.

Constraints, Approach and Present Status for Selecting the Mars Surveyor 2001 Landing Site

NASA Technical Reports Server (NTRS)

Golombek, M.; Anderson, F.; Bridges, N.; Briggs, G.; Gilmore, M.; Gulick, V.; Haldemann, A.; Parker, T.; Saunders, R.; Spencer, D.;

NASA's Chemical Transfer Propulsion Program for Pathfinder

NASA Technical Reports Server (NTRS)

Hannum, Ned P.; Berkopec, Frank D.; Zurawski, Robert L.

1989-01-01

Pathfinder is a research and technology project, with specific deliverables, initiated by the National Aeronautics and Space Administration (NASA) which will strengthen the technology base of the United States civil space program in preparation for future space exploration missions. Pathfinder begins in Fiscal Year 1989, and is to advance a collection of critical technologies for these missions and ensure technology readiness for future national decisions regarding exploration of the solar system. The four major thrusts of Pathfinder are: surface exploration, in-space operations, humans-in-space, and space transfer. The space transfer thrust will provide the critical technologies needed for transportation to, and return from, the Moon, Mars, and other planets in the solar system, as well as for reliable and cost-effective Earth-orbit operations. A key element of this thrust is the Chemical Transfer Propulsion program which will provide the propulsion technology for high performance, liquid oxygen/liquid hydrogen expander cycle engines which may be operated and maintained in space. Described here are the program overview including the goals and objectives, management, technical plan, and technology transfer for the Chemical Transfer Propulsion element of Pathfinder.

Free-Flight Experiments in LISA Pathfinder

NASA Technical Reports Server (NTRS)

Thorpe, J. I.; Cutler, C. J.; Hewitson, M.; Jennrich, O.; Maghami, P.; Paczkowski, S.; Russano, G.; Vitale, S.; Weber, W. J.

2014-01-01

The LISA Pathfinder mission will demonstrate the technology of drag-free test masses for use as inertial references in future space-based gravitational wave detectors. To accomplish this, the Pathfinder spacecraft will perform drag-free flight about a test mass while measuring the acceleration of this primary test mass relative to a second reference test mass. Because the reference test mass is contained within the same spacecraft, it is necessary to apply forces on it to maintain its position and attitude relative to the spacecraft. These forces are a potential source of acceleration noise in the LISA Pathfinder system that are not present in the full LISA configuration. While LISA Pathfinder has been designed to meet it's primary mission requirements in the presence of this noise, recent estimates suggest that the on-orbit performance may be limited by this 'suspension noise'. The drift-mode or free-flight experiments provide an opportunity to mitigate this noise source and further characterize the underlying disturbances that are of interest to the designers of LISA-like instruments. This article provides a high-level overview of these experiments and the methods under development to analyze the resulting data.

Lessons Learned from the Design, Certification, and Operation of the Space Shuttle Integrated Main Propulsion System (IMPS)

NASA Technical Reports Server (NTRS)

Martinez, Hugo E.; Albright, John D.; D'Amico, Stephen J.; Brewer, John M.; Melcher, John C., IV

2011-01-01

The Space Shuttle Integrated Main Propulsion System (IMPS) consists of the External Tank (ET), Orbiter Main Propulsion System (MPS), and Space Shuttle Main Engines (SSMEs). The IMPS is tasked with the storage, conditioning, distribution, and combustion of cryogenic liquid hydrogen (LH2) and liquid oxygen (LO2) propellants to provide first and second stage thrust for achieving orbital velocity. The design, certification, and operation of the associated IMPS hardware have produced many lessons learned over the course of the Space Shuttle Program (SSP). A subset of these items will be discussed in this paper for consideration when designing, building, and operating future spacecraft propulsion systems. This paper will focus on lessons learned related to Orbiter MPS and is the first of a planned series to address the subject matter.

Characterizing Intermediate-Mass, Pre-Main-Sequence Stars via X-Ray Emision

NASA Astrophysics Data System (ADS)

Haze Nunez, Evan; Povich, Matthew Samuel; Binder, Breanna Arlene; Broos, Patrick; Townsley, Leisa K.

2018-01-01

The X-ray emission from intermediate-mass, pre-main-sequence stars (IMPS) can provide useful constraints on the ages of very young (${<}5$~Myr) massive star forming regions. IMPS have masses between 2 and 8 $M_{\\odot}$ and are getting power from the gravitational contraction of the star. Main-sequence late-B and A-type stars are not expected to be strong X-ray emitters, because they lack the both strong winds of more massive stars and the magneto-coronal activity of lower-mass stars. There is, however, mounting evidence that IMPS are powerful intrinsic x-ray emitters during their convection-dominated early evolution, before the development and rapid growth of a radiation zone. We present our prime candidates for intrinsic, coronal X-ray emission from IMPS identified in the Chandra Carina Complex Project. The Carina massive star-forming complex is of special interest due to the wide variation of star formation stages within the region. Candidate IMPS were identified using infrared spectral energy distribution (SED) models. X-ray properties, including thermal plasma temperatures and absorption-corrected fluxes, were derived from XSPEC fits performed using absorption ($N_{H}$) constrained by the extinction values returned by the infrared SED fits. We find that IMPS have systematically higher X-ray luminosities compared to their lower-mass cousins, the TTauri stars.This work is supported by the National Science Foundation under grant CAREER-1454334 and by NASA through Chandra Award 18200040.

Emergence of Imipenem-Resistant Pseudomonas aeruginosa Clinical Isolates from Egypt Coharboring VIM and IMP Carbapenemases.

PubMed

El-Domany, Ramadan Ahmed; Emara, Mohamed; El-Magd, Mohammed A; Moustafa, Walaa H; Abdeltwab, Nesma M

2017-09-01

Pseudomonas aeruginosa is an important human pathogen and the leading cause of nosocomial infections. P. aeruginosa is characterized by massive intrinsic resistance to a multiple classes of antibiotics with carbapenems being the most potent inhibitor of P. aeruginosa and considered the first choice for its treatment. Therefore, it is crucial to investigate novel mechanisms of resistance of P. aeruginosa to carbapenems for achieving successful therapy. A total of 114 P. aeruginosa isolates from two university hospitals in Egypt were recruited in this study. Antimicrobial susceptibility testing revealed that 50 isolates (43.8%) exhibited multidrug-resistant (MDR) phenotype, of them 14 isolates (12.2%) were imipenem (IPM)-resistant. Of these 14 isolates, 13 isolates (11.4%) exhibited the metallo-β-lactamase (MBL) phenotype. MBLs encoding genes, VIM and IMP, were identified by PCR. PCR results revealed that four isolates harbored the VIM gene alone, one isolate harbored IMP gene alone, and four isolates harbored both genes. The correct size of PCR products of VIM and IMP genes (390 and 188 bp, respectively) were sequenced to confirm results of PCR and to look for any possible polymorphism among MBL genes of tested isolates. Data analysis of these sequences showed 100% identity of nucleotide sequences of MBL genes among tested Egyptian patients. To our knowledge, this is the first report of IMP carbapenemase-encoding gene in Africa and the first detection of the emergence of P. aeruginosa coproducing VIM and IMP genes in Egypt.

Preliminary Findings of the Photovoltaic Cell Calibration Experiment on Pathfinder Flight 95-3

NASA Technical Reports Server (NTRS)

Vargas-Aburto, Carlos

1997-01-01

The objective of the photovoltaic (PV) cell calibration experiment for Pathfinder was to develop an experiment compatible with an ultralight UAV to predict the performance of PV cells at AM0, the solar spectrum in space, using the Langley plot technique. The Langley plot is a valuable technique for this purpose and requires accurate measurements of air mass (pressure), cell temperature, solar irradiance, and current-voltage(IV) characteristics with the cells directed normal to the direct ray of the sun. Pathfinder's mission objective (95-3) of 65,000 ft. maximum altitude, is ideal for performing the Langley plot measurements. Miniaturization of electronic data acquisition equipment enabled the design and construction of an accurate and light weight measurement system that meets Pathfinder's low payload weight requirements.

MARS PATHFINDER LANDER REMOVED FROM SHIPPING CONTAINER IN SAEF-2

NASA Technical Reports Server (NTRS)

1996-01-01

In the SAEF-2 spacecraft checkout facility at Kennedy Space Center, engineers and technicians from Jet Propulsion Laboratory remove the Mars Pathfinder lander from its shipping container, still covered in protective wrapping. Pictured from L-R, Linda Robeck, Jerry Gutierrez, Lorraine Garcia, Chuck Foehlinger of JPL. The arrival of the spacecraft at KSC from Pasadena, CA occurred on Aug. 13, 1996. Launch of Mars Pathfinder aboard a McDonnell Douglas Delta II rocket will occur from Pad B at Complex 17 on Dec. 2.

Pathfinder aircraft in flight

NASA Image and Video Library

1995-07-27

The Pathfinder research aircraft's wing structure was clearly defined as it soared under a clear blue sky during a test flight July 27, 1995, from Dryden Flight Research Center, Edwards, California. The center section and outer wing panels of the aircraft had ribs constructed of thin plastic foam, while the ribs in the inner wing panels are fabricated from lightweight composite material. Developed by AeroVironment, Inc., the Pathfinder was one of several unmanned aircraft being evaluated under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program.

Mars Pathfinder Microrover- Implementing a Low Cost Planetary Mission Experiment

NASA Technical Reports Server (NTRS)

Matijevic, J.

1996-01-01

The Mars Pathfinder Microrover Flight Experiment (MFEX) is a NASA Office of Space Access and Technology (OSAT) flight experiment which has been delivered and integrated with the Mars Pathfinder (MPF) lander and spacecraft system. The total cost of the MFEX mission, including all subsystem design and development, test, integration with the MPF lander and operations on Mars has been capped at $25 M??is paper discusses the process and the implementation scheme which has resulted in the development of this first Mars rover.

MARS PATHFINDER AIR BAG INSTALLATION IN SAEF-2

NASA Technical Reports Server (NTRS)

1996-01-01

In the Spacecraft Assembly and Encapsulation Facility-2 (SAEF-2), the Jet Propulsion Laboratory (JPL) team installs air bags on the Mars Pathfinder lander. The four airbags will cushion the lander as it touches down on the Martian surface, protecting the delicate instruments and Surveyor small rover inside the tetrahedral-shaped lander. The Mars Pathfinder is one of two Mars-bound spacecraft being prepared for launch this fall. Liftoff is set for Dec. 2 at the beginning of a 24-day launch period.

Increased skin permeation efficiency of imperatorin via charged ultradeformable lipid vesicles for transdermal delivery

PubMed Central

Lin, Hongwei; Xie, Qingchun; Huang, Xin; Ban, Junfeng; Wang, Bo; Wei, Xing

2018-01-01

Aim The aim of this work was to develop a novel vesicular carrier, ultradeformable liposomes (UDLs), to expand the applications of the Chinese herbal medicine, imperatorin (IMP), and increase its transdermal delivery. Methods In this study, we prepared IMP-loaded UDLs using the thin-film hydration method and evaluated their encapsulation efficiency, vesicle deformability, skin permeation, and the amounts accumulated in different depths of the skin in vitro. The influence of different charged surfactants on the properties of the UDLs was also investigated. Results The results showed that the UDLs containing cationic surfactants had high entrapment efficiency (60.32%±2.82%), an acceptable particle size (82.4±0.65 nm), high elasticity, and prolonged drug release. The penetration rate of IMP in cationic-UDLs was 3.45-fold greater than that of IMP suspension, which was the highest value among the vesicular carriers. UDLs modified with cationic surfactant also showed higher fluorescence intensity in deeper regions of the epidermis. Conclusion The results of our study suggest that cationic surfactant-modified UDLs could increase the transdermal flux, prolong the release of the drug, and serve as an effective dermal delivery system for IMP. PMID:29467573

Oligomerization reactions of ribonucleotides - The reaction of the 5'-phosphorimidazolide of nucleosides on montmorillonite and other minerals

NASA Technical Reports Server (NTRS)

Ferris, James P.; Ertem, Gozen

1992-01-01

The reaction of ImpA in the presence of Na(+)-montmorillonite 22A or Na(+)-Volclay in aqueous, pH 8 solution gives a 50-60 percent yield of dimers and trimers (pA)2 and (pA)3. The ratio of 3',5'-phosphodiester bond formation is twice as great as 2',5'-bond formation. The reaction requires the presence of Mg2+ and is inhibited by 0.4 M imidazole. N-methylimidazole enhances the rate of the reaction but does not cause major changes in yield or product composition. Higher yields were obtained when Li+- or Ca2+-montmorillonites were used in place of Na+-montmorillonite. Little or no phosphodiester bond formation was observed with Mg2+- or Al3+-montmorillonite. Montmorillonites other than 22A and Volclay exhibited litle or no catalysis. In addittion, little or no catalysis was exhibited in ferrugenous smectite, nontronite, allophane, imogolite or sepiolite. Oligomers were also formed by the reaction of ImpG, 2-methylImpG, ImpC and ImpU in the presence of Na+-montmorillonite. The pyrimidine nucleotides gave significantly lower yields of oligomers.

Increased skin permeation efficiency of imperatorin via charged ultradeformable lipid vesicles for transdermal delivery.

PubMed

Lin, Hongwei; Xie, Qingchun; Huang, Xin; Ban, Junfeng; Wang, Bo; Wei, Xing; Chen, Yanzhong; Lu, Zhufen

2018-01-01

The aim of this work was to develop a novel vesicular carrier, ultradeformable liposomes (UDLs), to expand the applications of the Chinese herbal medicine, imperatorin (IMP), and increase its transdermal delivery. In this study, we prepared IMP-loaded UDLs using the thin-film hydration method and evaluated their encapsulation efficiency, vesicle deformability, skin permeation, and the amounts accumulated in different depths of the skin in vitro. The influence of different charged surfactants on the properties of the UDLs was also investigated. The results showed that the UDLs containing cationic surfactants had high entrapment efficiency (60.32%±2.82%), an acceptable particle size (82.4±0.65 nm), high elasticity, and prolonged drug release. The penetration rate of IMP in cationic-UDLs was 3.45-fold greater than that of IMP suspension, which was the highest value among the vesicular carriers. UDLs modified with cationic surfactant also showed higher fluorescence intensity in deeper regions of the epidermis. The results of our study suggest that cationic surfactant-modified UDLs could increase the transdermal flux, prolong the release of the drug, and serve as an effective dermal delivery system for IMP.

Ras regulates assembly of mitogenic signalling complexes through the effector protein IMP.

PubMed

Matheny, Sharon A; Chen, Chiyuan; Kortum, Robert L; Razidlo, Gina L; Lewis, Robert E; White, Michael A

2004-01-15

The signal transduction cascade comprising Raf, mitogen-activated protein (MAP) kinase kinase (MEK) and MAP kinase is a Ras effector pathway that mediates diverse cellular responses to environmental cues and contributes to Ras-dependent oncogenic transformation. Here we report that the Ras effector protein Impedes Mitogenic signal Propagation (IMP) modulates sensitivity of the MAP kinase cascade to stimulus-dependent activation by limiting functional assembly of the core enzymatic components through the inactivation of KSR, a scaffold/adaptor protein that couples activated Raf to its substrate MEK. IMP is a Ras-responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination, which releases the inhibition of Raf-MEK complex formation. Thus, Ras activates the MAP kinase cascade through simultaneous dual effector interactions: induction of Raf kinase activity and derepression of Raf-MEK complex formation. IMP depletion results in increased stimulus-dependent MEK activation without alterations in the timing or duration of the response. These observations suggest that IMP functions as a threshold modulator, controlling sensitivity of the cascade to stimulus and providing a mechanism to allow adaptive behaviour of the cascade in chronic or complex signalling environments.

Pathfinder Innovation Projects

EPA Pesticide Factsheets

The Pathfinder program supports high-risk, high-reward research ideas with funding and staff time. The goal is to feed a culture of innovation in the Agency and integrate innovative ideas in EPA research programs.

Predominance of carbapenem-resistant Pseudomonas aeruginosa isolates carrying blaIMP and blaVIM metallo-β-lactamases in a major hospital in Costa Rica.

PubMed

Toval, Francisco; Guzmán-Marte, Anel; Madriz, Vivian; Somogyi, Teresita; Rodríguez, César; García, Fernando

2015-01-01

This study aimed to assess the molecular basis of the resistance to carbapenems in clinical isolates of Pseudomonas aeruginosa recovered from a tertiary-level health facility in San José, Costa Rica. A total of 198 non-duplicated isolates were evaluated for their susceptibility to β-lactams, aminoglycosides and fluoroquinolones. The production of metallo-β-lactamases (MBLs), the presence of MBL encoding genes (blaIMP, blaVIM and blaGIM-1) and the occurrence of these genes within class 1 integrons were investigated. In addition, an ERIC2 PCR fingerprinting method was used to elucidate the distribution of the detected MBL genes within the strain collection. Of the 198 isolates tested, 125 (63.1 %) were categorized as carbapenem-resistant. The majority (88.8 %) of the carbapemen-resistant isolates also showed resistance to ceftazidime, cefepime, aztreonam, ticarcillin/clavulanic acid, amikacin, gentamicin, tobramycin, ciprofloxacin and gatifloxacin. Among the carbapenem-resistant isolates, 102 (81.6 %) showed MBL activity. Strikingly, both blaIMP and blaVIM genes were simultaneously detected in most (94.1 %) of the 102 MBL producers. Five carbapenem-resistant MBL producers were positive only for blaIMP genes. Almost 70 % of the isolates examined harboured the intI1 gene, accompanied by the sul1 and qacEΔ1 genes in 136 (99 %) and 122 (89 %) isolates, respectively. The majority (94.4 %) of the carbapenem-resistant isolates carried the intI1 gene, in contrast to 26 % of the carbapenem-susceptible isolates. Ninety-three out of 96 (96.9 %) isolates carrying both blaIMP and blaVIM genes also harboured the intI1, sul1 and qacEΔ1 genes. Gene cassettes from carbapenem-susceptible and MBL-negative carbapenem-resistant isolates encoded aminoglycoside-resistance enzymes (aadA2, aadA4 and aadA6) as well as orfD and qacF genes. RAPD analysis distributed 126 of the isolates in 29 clusters. Eighty of the 90 blaIMP (+) blaVIM (+) isolates were sorted into 16 different clusters, suggesting that the blaIMP and blaVIM genes detected were located within a genetic element capable of lateral transfer. Carbapenem-resistant MBL-positive isolates were recovered from almost all hospital wards and were over-represented in samples obtained from the surgical emergency and intensive care therapy units. Remarkably, three carbapenem-resistant isolates, exhibiting MBL activity and carrying both blaIMP and blaVIM genes, were recovered from outpatients. Sequence analysis of both bla genes in various isolates revealed that they correspond to the alleles blaIMP-18 and blaVIM-2. To our knowledge, this is the first report of the combination of two metallo-β-lactamases encoded by the blaIMP-18 and blaVIM-2 genes in P. aeruginosa. © 2015 The Authors.

Insulin-like growth factor II mRNA-binding protein 3 (IMP3) is a marker that predicts presence of invasion in papillary biliary tumors.

PubMed

Sasaki, Motoko; Sato, Yasunori

2017-04-01

Biliary tumors showing intraductal papillary growth (Pap-BTs) include intraductal papillary neoplasm of the bile duct (IPNB) and papillary cholangiocarcinoma (CC). A differential diagnosis between IPNB and papillary CC currently remains challenging. The aim of the present study is to identify histological features and immunohistochemical markers of malignant potential such as tumor invasion in Pap-BTs. Subjects comprised 37 patients with Pap-BT (intrahepatic and perihilar [proximal], 27: 17 noninvasive and 10 invasive; distal, 10: all invasive). We examined histological features and the expression of p53, enhancer of zeste homolog 2, insulin-like growth factor II mRNA-binding protein 3 (IMP3), and DNA methyltransferase-1 in the intraductal area in Pap-BTs. Noninvasive Pap-BT was characterized by the presence of a low-grade dysplastic area, edematous stroma, and the absence of necrosis. The expression of p53, enhancer of zeste homolog 2, IMP3, and DNA methyltransferase-1 was significantly weaker in noninvasive Pap-BTs than in invasive Pap-BTs (P<.01). Diffuse cytoplasmic IMP3 expression was absent in noninvasive Pap-BTs. IMP3 showed the greatest specificity to predict a presence of invasion. A heatmap demonstrated that proximal noninvasive Pap-BTs and distal Pap-BTs may be completely different. In bile duct biopsies, the expression of IMP3 was the most precise predictor of invasion in Pap-BTs. In conclusion, Pap-BTs may be separated into 3 subgroups: (1) proximal noninvasive Pap-BT, corresponding to IPNB; (2) distal invasive Pap-BT, corresponding to papillary CC; and (3) the remaining Pap-BT including IPNB with associated adenocarcinomas, based on histological and immunohistochemical features. IMP3 may be a useful marker for predicting invasion in Pap-BT. Copyright © 2017 Elsevier Inc. All rights reserved.

ISTP observations of plasmoid ejection: IMP 8 and Geotail

DOE Office of Scientific and Technical Information (OSTI.GOV)

Slavin, J.A.; Fairfield, D.H.; Kuznetsova, M.M.

1998-01-01

IMP 8 and Geotail observations of traveling compression regions (TCRs) and plasmoids, respectively, are used to investigate plasmoid formation and ejection. One year of IMP 8 magnetometer measurements taken during the distant tail phase of the Geotail mission were searched for TCRs, which signal the release of plasmoids down the tail. A total of 10 such intervals were identified. Examination of the Geotail measurements showed that this spacecraft was in the magnetotail for only three of the events. However, in all three cases, clear plasmoid signatures were observed at Geotail. These plasmoids were observed at distances of X={minus}170 to {minus}197more » R{sub E}. The in situ plasma velocities in these plasmoids are found to exceed the time-of-flight speeds between IMP 8 and Geotail suggesting that some further acceleration may have taken place following release. The inferred lengths of these plasmoids, {approximately}27{endash}40 R{sub E}, are comparable to the downtail distance of IMP 8. This indicates that TCR at IMP 8 can be caused by plasmoids forming not only earthward but also adjacent to or just tailward of the spacecraft. The closeness of IMP 8 to the point of plasmoid formation is confirmed by the small, {approximately}0{endash}3min, time delays between the TCR perturbation and substorm onset. In two of the plasmoid events, high-speed earthward plasma flows and streaming energetic particles were measured in the plasma sheet boundary layer surrounding the plasmoid along with large positive B{sub z} at the leading edge of the plasmoid suggesting that the core of the plasmoid was {open_quotes}snow plowing{close_quotes} into flux tubes recently closed at an active distant neutral line. In summary, these unique two-point measurements clearly show plasmoid ejection near substorm onset, their rapid movement to the distant tail and their further evolution as they encounter preexisting X lines in the distant tail. {copyright} 1998 American Geophysical Union« less

Overexpression of the OsIMP Gene Increases the Accumulation of Inositol and Confers Enhanced Cold Tolerance in Tobacco through Modulation of the Antioxidant Enzymes' Activities.

PubMed

Zhang, Rong-Xiang; Qin, Li-Jun; Zhao, De-Gang

2017-07-20

Inositol is a cyclic polyol that is involved in various physiological processes, including signal transduction and stress adaptation in plants. l- myo -inositol monophosphatase (IMPase) is one of the metal-dependent phosphatase family members and catalyzes the last reaction step of biosynthesis of inositol. Although increased IMPase activity induced by abiotic stress has been reported in chickpea plants, the role and regulation of the IMP gene in rice ( Oryza sativa L.) remains poorly understood. In the present work, we obtained a full-length cDNA sequence coding IMPase in the cold tolerant rice landraces in Gaogonggui, which is named as OsIMP . Multiple alignment results have displayed that this sequence has characteristic signature motifs and conserved enzyme active sites of the phosphatase super family. Phylogenetic analysis showed that IMPase is most closely related to that of the wild rice Oryza brachyantha , while transcript analysis revealed that the expression of the OsIMP is significantly induced by cold stress and exogenous abscisic acid (ABA) treatment. Meanwhile, we cloned the 5' flanking promoter sequence of the OsIMP gene and identified several important cis -acting elements, such as LTR (low-temperature responsiveness), TCA-element (salicylic acid responsiveness), ABRE-element (abscisic acid responsiveness), GARE-motif (gibberellin responsive), MBS (MYB Binding Site) and other cis -acting elements related to defense and stress responsiveness. To further investigate the potential function of the OsIMP gene, we generated transgenic tobacco plants overexpressing the OsIMP gene and the cold tolerance test indicated that these transgenic tobacco plants exhibit improved cold tolerance. Furthermore, transgenic tobacco plants have a lower level of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA), and a higher content of total chlorophyll as well as increased antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), when compared to wild type (WT) tobacco plants under normal and cold stress conditions.

Structure-function studies of adenylosuccinate synthetase from Escherichia coli.

PubMed

Honzatko, R B; Fromm, H J

1999-10-01

Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, thermodynamically coupling the hydrolysis of GTP to the formation of adenylosuccinate from l-aspartate and IMP. The enzyme from Esherichia coli undergoes a ligand-induced dimerization, which leads to the assembly of a complete active site. The binding of IMP causes conformational changes over distances of 30 A, the end result of which is the activation of essential catalytic elements and the organization of the binding pocket for Mg(2+)-GTP. The enzyme promotes first a phosphoryl transfer from GTP to the 6-oxygen atom of IMP, by way of a transition state that has characteristics of both associative and dissociative reaction pathways. Following the formation of 6-phosphoryl-IMP, the enzyme then catalyzes the nucleophilic displacement of the 6-phosphoryl group by the alpha-amino group of l-aspartate in a transition state, which requires two metal cations. Copyright 1999 Academic Press.

Extendible-retractable electric field measurement antenna for IMP J

NASA Technical Reports Server (NTRS)

Larrick, W.

1973-01-01

An antenna dispenser mechanism for the IMP J spacecraft was designed, fabricated, and tested. Upon command the mechanism deploys or retracts a conductor for use as a receiving antenna for an electric field measurement experiment. Five identical units were fabricated and tested to the IMP H & J environmental test specification. Of these, four are designated for flight on the IMP J spacecraft and one as a prototype flight spare. The testing program was successfully completed although certain design modifications were required as problems were uncovered by the testing; particularly thermal vacuum operation. The antenna mechanism functions well under the expected environmental and loading conditions. The wear life and load capability of the dry molybdenum disulphide lubricant originally used on the heavily loaded worm and gear pair were disappointing and a substitute material was applied. The lubricant finally applied performed well; although other problems were generated.

Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins

PubMed Central

Verardi, Raffaello; Traaseth, Nathaniel J.; Masterson, Larry R.; Vostrikov, Vitaly V.; Veglia, Gianluigi

2013-01-01

In this chapter, we summarize the isotopic labeling strategies used to obtain high-quality solution and solid-state NMR spectra of biological samples, with emphasis on integral membrane proteins (IMPs). While solution NMR is used to study IMPs under fast tumbling conditions, such as in the presence of detergent micelles or isotropic bicelles, solid-state NMR is used to study the structure and orientation of IMPs in lipid vesicles and bilayers. In spite of the tremendous progress in biomolecular NMR spectroscopy, the homogeneity and overall quality of the sample is still a substantial obstacle to overcome. Isotopic labeling is a major avenue to simplify overlapped spectra by either diluting the NMR active nuclei or allowing the resonances to be separated in multiple dimensions. In the following we will discuss isotopic labeling approaches that have been successfully used in the study of IMPs by solution and solid-state NMR spectroscopy. PMID:23076578

Immersive Environments for Mission Operations: Beyond Mars Pathfinder

NASA Technical Reports Server (NTRS)

Wright, J.; Hartman, F.; Cooper, B.

1998-01-01

Immersive environments are just beginning to be used to support mission operations at the Jet Propulsion Laboratory. This technology contributed to the Mars Pathfinder Mission in planning sorties for the Sojourner rover.

Pathfinder Innovation Projects: Awardees 2015

EPA Pesticide Factsheets

The Pathfinder program supports high-risk, high-reward research ideas with funding and staff time. The goal is to feed a culture of innovation in the Agency and integrate innovative ideas in EPA research programs.

Pathfinder Innovation Projects: Awardees 2016

EPA Pesticide Factsheets

The Pathfinder program supports high-risk, high-reward research ideas with funding and staff time. The goal is to feed a culture of innovation in the Agency and integrate innovative ideas in EPA research programs.

Dimerization in Highly Concentrated Solutions of Phosphoimidazolide Activated Monomucleotides

NASA Astrophysics Data System (ADS)

Kanavarioti, Anastassia

1997-08-01

Phosphoimidazolide activated ribomononucleotides (*pN) are useful substrates for the non-enzymatic synthesis of polynucleotides. However, dilute neutral aqueous solutions of *pN typically yield small amounts of dimers and traces of polymers; most of *pN hydrolyzes to yield nucleoside 5'-monophosphate. Here we report the self-condensation of nucleoside 5'-phosphate 2-methylimidazolide (2-MeImpN with N = cytidine, uridine or guanosine) in the presence of Mg2+ in concentrated solutions, such as might have been found in an evaporating lagoon on prebiotic Earth. The product distribution indicates that oligomerization is favored at the expense of hydrolysis. At 1.0 M, 2-MeImpU and 2-MeImpC produce about 65% of oligomers including 4% of the 3',5'-linked dimer. Examination of the product distribution of the three isomeric dimers in a self-condensation allows identification of reaction pathways that lead to dimer formation. Condensations in a concentrated mixture of all three nucleotides (U,C,G mixtures) is made possible by the enhanced solubility of 2-MeImpG in such mixtures. Although percent yield of internucleotide linked dimers is enhanced as a function of initial monomer concentration, pyrophosphate dimer yields remain practically unchanged at about 20% for 2-MeImpU, 16% for 2-MeImpC and 25% of the total pyrophosphate in the U,C,G mixtures. The efficiency by which oligomers are produced in these concentrated solutions makes the evaporating lagoon scenario a potentially interesting medium for the prebiotic synthesis of dimers and short RNAs.

Access to investigational medicinal products for minors in Europe: ethical and regulatory issues in negotiating children's access to investigational medicines.

PubMed

Pinxten, Wim; Nys, Herman; Dierickx, Kris

2010-12-01

Patients who search for a better treatment, an increased quality of life, or even a chance to preserve life itself may claim to have an interest in accessing investigational medicinal products (IMP), particularly when no validated treatment for their disease or condition exists. For many, awaiting the uncertain and time-consuming process of converting an IMP into an approved drug may not appear a realistic option, as prognoses may be grim and a dramatic outcome may seem hard to avert. Gaining access to an IMP, however, often proves to be a difficult enterprise with a highly uncertain outcome. In addition, the process of seeking access to IMP is surrounded by various ethical issues that will be explored in this article. This paper explores the ethical concerns in two potential tracks of seeking access to IMP for minors: on an individual basis, or collectively, as a patient organisation. In this discourse, several unique ethical and regulatory concerns related to the direct negotiation of access to IMP for minor patients are identified, with a focus on product safety, the recruitment of research subjects, the unnoticed entry of market mechanisms in the recruitment of research subjects, and the sidelining of third parties in the recruitment process. The paper concludes with a concise reflection on the way forward. The quest for access to investigational drugs is particularly relevant to paediatric practice, in which a significant share of the drugs prescribed has never been tested in children or labelled for use in the paediatric population.

Constraints, Approach, and Status of Mars Surveyor 2001 Landing Site Selection

NASA Technical Reports Server (NTRS)

Golombek, M.; Bridges, N.; Briggs, G.; Gilmore, M.; Haldemann, A.; Parker, T.; Saunders, R.; Spencer, D.; Smith, J.; Soderblom, L.

1999-01-01

There are many similarities between the Mars Surveyor '01 (MS '01) landing site selection process and that of Mars Pathfinder. The selection process includes two parallel activities in which engineers define and refine the capabilities of the spacecraft through design, testing and modeling and scientists define a set of landing site constraints based on the spacecraft design and landing scenario. As for Pathfinder, the safety of the site is without question the single most important factor, for the simple reason that failure to land safely yields no science and exposes the mission and program to considerable risk. The selection process must be thorough and defensible and capable of surviving multiple withering reviews similar to the Pathfinder decision. On Pathfinder, this was accomplished by attempting to understand the surface properties of sites using available remote sensing data sets and models based on them. Science objectives are factored into the selection process only after the safety of the site is validated. Finally, as for Pathfinder, the selection process is being done in an open environment with multiple opportunities for community involvement including open workshops, with education and outreach opportunities. Additional information is contained in the original extended abstract.

International Space Station Instmments Collect Imagery of Natural Disasters

NASA Technical Reports Server (NTRS)

Evans, C. A.; Stefanov, W. L.

2013-01-01

A new focus for utilization of the International Space Station (ISS) is conducting basic and applied research that directly benefits Earth's citizenry. In the Earth Sciences, one such activity is collecting remotely sensed imagery of disaster areas and making those data immediately available through the USGS Hazards Data Distribution System, especially in response to activations of the International Charter for Space and Major Disasters (known informally as the "International Disaster Charter", or IDC). The ISS, together with other NASA orbital sensor assets, responds to IDC activations following notification by the USGS. Most of the activations are due to natural hazard events, including large floods, impacts of tropical systems, major fires, and volcanic eruptions and earthquakes. Through the ISS Program Science Office, we coordinate with ISS instrument teams for image acquisition using several imaging systems. As of 1 August 2013, we have successfully contributed imagery data in support of 14 Disaster Charter Activations, including regions in both Haiti and the east coast of the US impacted by Hurricane Sandy; flooding events in Russia, Mozambique, India, Germany and western Africa; and forest fires in Algeria and Ecuador. ISS-based sensors contributing data include the Hyperspectral Imager for the Coastal Ocean (HICO), the ISERV (ISS SERVIR Environmental Research and Visualization System) Pathfinder camera mounted in the US Window Observational Research Facility (WORF), the ISS Agricultural Camera (ISSAC), formerly operating from the WORF, and high resolution handheld camera photography collected by crew members (Crew Earth Observations). When orbital parameters and operations support data collection, ISS-based imagery adds to the resources available to disaster response teams and contributes to the publicdomain record of these events for later analyses.

Improved iodine radiolabels for monoclonal antibody therapy.

PubMed

Stein, Rhona; Govindan, Serengulam V; Mattes, M Jules; Chen, Susan; Reed, Linda; Newsome, Guy; McBride, Bill J; Griffiths, Gary L; Hansen, Hans J; Goldenberg, David M

2003-01-01

A major disadvantage of (131)iodine (I)-labeled monoclonal antibodies (MAbs) for radioimmunotherapy has been the rapid diffusion of iodotyrosine from target cells after internalization and catabolism of the radioiodinated MAbs. We recently reported that a radioiodinated, diethylenetriaminepentaacetic acid-appended peptide, designated immunomedics' residualizing peptide 1 (IMP-R1), was a residualizing iodine label that overcame many of the limitations that had impeded the development of residualizing iodine for clinical use. To determine the factors governing the therapeutic index of the labeled MAb, as well as the factors required for production of radioiodinated MAb in high yield and with high specific activity, variations in the peptide structure of IMP-R1 were evaluated. A series of radioiodinated, diethylenetriaminepentaacetic acid-appended peptide moieties (IMP-R1 through IMP-R8) that differed in overall hydrophilicity and charge were compared. Radioiodinations of the peptides followed by conjugations to disulfide-reduced RS7 (an anti-epithelial glycoprotein-1 MAb) furnished radioimmunoconjugates in good overall incorporations, with immunoreactivities comparable to that of directly radioiodinated RS7. Specific activities of up to 8 mCi/mg and yields > 80% have been achieved. In vitro processing experiments showed marked increases in radioiodine retention with all of the adducts; radioiodine retention at 45 h was up to 86% greater in cells than with directly iodinated RS7. Each of the (125)I-peptide-RS7 conjugates was compared with (131)I-RS7 (labeled by the chloramine-T method) in paired-label biodistribution studies in nude mice bearing human lung tumor xenografts. All of the residualizing substrates exhibited significantly enhanced retention in tumor in comparison to directly radioiodinated RS7, but the nontarget uptakes differed significantly among the residualizing labels. The best labels were IMP-R4 and IMP-R8, showing superior tumor-to-non-tumor ratios by virtue of high tumor uptake and retention and low normal organ uptake, as well as superior radiochemical properties. The therapeutic efficacy of (131)I-IMP-R4-RS7 was compared with that of conventionally (131)I-labeled RS7 and (90)yttrium-RS7 in the nude mice lung cancer model. The therapeutic efficacy of (131)I-IMP-R4-RS7 and (90)yttrium-RS7 were equivalent, and both agents yielded significantly improved control of tumor growth compared with conventional (131)I-labeled RS7.

Operations and Autonomy of the Mars Pathfinder Microrover

NASA Technical Reports Server (NTRS)

Mishkin, A. H.; Morrison, J. C.; Nguyen, T. T.; Stone, H. W.; Cooper, B. K.

1998-01-01

The Microrover Flight Experiment (MFEX) is a NSAS OACT (Office of Advanced Concepts and Technology) flight experiment which, integrated with the Mars Pathfinder (MPF) lander and spacecraft system, landed on Mars on July 4, 1997.

Lithium-Thionyl Chloride Batteries for the Mars Pathfinder Microrover

NASA Technical Reports Server (NTRS)

Deligiannis, Frank; Frank, Harvey; Staniewicz, R. J.; Willson, John

1996-01-01

A discussion of the power requirements for the Mars Pathfinder Mission is given. Topics include: battery requirements; cell design; battery design; test descriptions and results. A summary of the results is also included.

Pathfinder on lakebed rolling out for test flight

NASA Image and Video Library

1995-12-10

The Pathfinder research aircraft's wing structure is clearly defined in this photo as personnel from AeroVironment rolled it out onto the lakebed at NASA's Dryden Flight Research Center, Edwards, California, for another test flight.

Determination of trace element mineral/liquid partition coefficients in melilite and diopside by ion and electron microprobe techniques

NASA Technical Reports Server (NTRS)

Kuehner, S. M.; Laughlin, J. R.; Grossman, L.; Johnson, M. L.; Burnett, D. S.

1989-01-01

The applicability of ion microprobe (IMP) for quantitative analysis of minor elements (Sr, Y, Zr, La, Sm, and Yb) in the major phases present in natural Ca-, Al-rich inclusions (CAIs) was investigated by comparing IMP results with those of an electron microprobe (EMP). Results on three trace-element-doped glasses indicated that it is not possible to obtain precise quantitative analysis by using IMP if there are large differences in SiO2 content between the standards used to derive the ion yields and the unknowns.

Importin-7 Mediates Nuclear Trafficking of DNA in Mammalian Cells

PubMed Central

Dhanoya, Arjun; Wang, Tse; Keshavarz-Moore, Eli; Fassati, Ariberto; Chain, Benjamin M

2013-01-01

Eukaryotic cells have the ability to uptake and transport endogenous and exogenous DNA in their nuclei, however little is known about the specific pathways involved. Here we show that the nuclear transport receptor importin 7 (imp7) supports nuclear import of supercoiled plasmid DNA and human mitochondrial DNA in a Ran and energy-dependent way. The imp7-dependent pathway was specifically competed by excess DNA but not by excess of maltose-binding protein fused with the classical nuclear localizing signal (NLS) or the M9 peptides. Transport of DNA molecules complexed with poly-l-lysine was impaired in intact cells depleted of imp7, and DNA complexes remained localized in the cytoplasm. Poor DNA nuclear import in cells depleted of imp7 directly correlated with lower gene expression levels in these cells compared to controls. Inefficient nuclear import of transfected DNA induced greater upregulation of the interferon pathway, suggesting that rapid DNA nuclear import may prevent uncontrolled activation of the innate immune response. Our results provide evidence that imp7 is a non-redundant component of an intrinsic pathway in mammalian cells for efficient accumulation of exogenous and endogenous DNA in the nucleus, which may be critical for the exchange of genetic information between mitochondria and nuclear genomes and to control activation of the innate immune response. PMID:23067392

Testing Gender Effects on the Mechanisms Explaining the Association between Post-Traumatic Stress Symptoms and Substance Use Frequency

PubMed Central

Bornovalova, Marina A.; Ouimette, Paige; Crawford, Aaron V.; Levy, Roy

2009-01-01

The present study examines gender differences in the mechanisms that explain the association between PTSD symptoms and substance use frequency in a sample of 182 urban substance users. Specifically, the current study examined gender differences in the role of two potential explanatory variables, namely, difficulties controlling impulsive behavior when distressed (IMP), and a lack of emotional awareness and clarity (AW/CLAR). Multiple-group path modeling (across males and females) was used to examine gender differences in the path coefficients from PTSD symptoms to IMP and AW/CLAR, and from these latter variables to drug use frequency. Results indicated that PTSD symptoms were associated with IMP and AW/CLAR, and these path coefficients did not vary by gender. However, gender differences emerged when considering the path coefficients from AW/CLAR and IMP to substance use frequency. Specifically, for women, the association between PTSD and substance use was partially explained by IMP, whereas for men, the association between PTSD and substance use was partially explained by AW/CLAR. The current study is the first to examine gender differences in mechanisms accounting for the association between PTSD and substance use frequency, and these results also support the value and importance of examining gender differences in mechanisms underlying PTSD-SUD comorbidity. PMID:19423233

Rearing practices and impulsivity/hyperactivity symptoms in relation to inflated responsibility and obsessive-compulsive symptoms.

PubMed

Smári, Jakob; Martinsson, Davíð Rúrik; Einarsson, Hjalti

2010-10-01

The aim of the study was to investigate potential precursors of inflated responsibility (responsibility attitudes) and obsessive-compulsive (OCD) symptoms. It was argued that both parental overprotection and impulsivity, separately and in interaction with each other, contribute to inflated responsibility and OCD symptoms. In a large sample of young adults (N = 570), self-report measures of OCD symptoms (OCI-R), responsibility attitudes (RAS), anxiety/depression (HADS), rearing practices (EMBU), present and past impulsivity/hyperactivity symptoms (IMP/HY) were administered. Overprotection as well as IMP/HY were found to predict OCD symptoms as well as inflated responsibility. Finally, a significant interaction was found between IMP/HY and overprotection with regard to both OCD symptoms and inflated responsibility. This effect reflected that IMP/HY was more strongly related to OCD symptoms and responsibility in people who had not been overprotected than in people who had been. Conversely overprotection was related to OCD symptoms and responsibility in people low but not in people high in IMP/HY. The results seem to indicate that the inadequacy between offer and need for parental control may play a role in the development of OCD symptoms. © 2010 The Authors. Scandinavian Journal of Psychology © 2010 The Scandinavian Psychological Associations.

Processing and Analysis of Mars Pathfinder Science Data at JPL's Science Data Processing Section

NASA Technical Reports Server (NTRS)

LaVoie, S.; Green, W.; Runkle, A.; Alexander, D.; Andres, P.; DeJong, E.; Duxbury, E.; Freda, D.; Gorjian, Z.; Hall, J.;

Sojourner Sits Near "Rock Garden"

NASA Image and Video Library

2003-02-01

The Mars Pathfinder Rover Sojourner images by the Imager for Mars Pathfinder as it nears the rock "Wedge." Part of the Rock Garden is visible in the upper right of the image. http://photojournal.jpl.nasa.gov/catalog/PIA04318

Analysis of Mars Pathfinder Entry Data, Aerothermal Heating, and Heat Shield Material Response

NASA Technical Reports Server (NTRS)

Milos, Frank; Chen, Y. K.; Tran, H. K.; Rasky, Daniel J. (Technical Monitor)

1997-01-01

The Mars Pathfinder heatshield contained several thermocouples and resistance thermometers. A description of the experiment, the entry data, and analysis of the entry environment and material response is presented. In particular, the analysis addresses uncertainties of the data and the fluid dynamics and material response models. The calculations use the latest trajectory and atmosphere reconstructions for the Pathfinder entry. A modified version of the GIANTS code is used for CFD (computational fluid dynamics) analyses, and FIAT is used for material response. The material response and flowfield are coupled appropriately. Three different material response models are considered. The analysis of Pathfinder entry data for validation of aerothermal heating and material response models is complicated by model uncertainties and unanticipated data-acquisition and processing problems. We will discuss these issues as well as ramifications of the data and analysis for future Mars missions.

Pathfinder technologies for bold new missions. [U.S. research and development program for space exploration

NASA Technical Reports Server (NTRS)

Sadin, Stanley R.; Rosen, Robert

1987-01-01

Project Pathfinder is a proposed U.S. Space Research and Technology program intended to enable bold new missions of space exploration. Pathfinder continues the advancement of technological capabilities and extends the foundation established under the Civil Space Technology Initiative, CSTI. By filling critical technological gaps, CSTI enhances access to Earth orbit and supports effective operations and science missions therein. Pathfinder, with a longer-term horizon, looks to a future that builds on Shuttle and Space Station and addresses technologies that support a range of exploration missions including: a return to the Moon to build an outpost; piloted missions to Mars; and continued scientific exploration of Earth and the other planets. The program's objective is to develop, within reasonable time frames, those emerging and innovative technologies that will make possible both new and enhanced missions and system concepts.

AVHRR-Based Polar Pathfinder Products: Evaluation, Enhancement and Transition to MODIS

NASA Technical Reports Server (NTRS)

Fowler, Charles; Masalanik, James; Stone, Robert; Stroeve, Julienne; Emery, William

2001-01-01

The Advanced Very High Resolution Radiometer (AVHRR)-Based Polar Pathfinder (APP) products include calibrated AVHRR channel data, surface temperatures, albedo, satellite scan and solar geometries, and cloud mask, all composited into twice-per-day images, and daily averaged fields of sea ice motion, for regions poleward of 50 latitude. Our general goals under this grant: (1) Quantify the APP accuracy and sources of error by comparing Pathfinder products with field measurements; (2) Determine the consistency of mean fields and trends in comparison with longer time series of available station data and forecast model output; (3) Investigate the consistency of the products between the different AVHRR instruments over the 1982-present period of the NOAA program; and (4) Compare and annual cycle of the APP products with MODIS to establish a baseline for extending Pathfinder-type products into the new ESE period.

Modeling to Mars: a NASA Model Based Systems Engineering Pathfinder Effort

NASA Technical Reports Server (NTRS)

Phojanamongkolkij, Nipa; Lee, Kristopher A.; Miller, Scott T.; Vorndran, Kenneth A.; Vaden, Karl R.; Ross, Eric P.; Powell, Bobby C.; Moses, Robert W.

2017-01-01

The NASA Engineering Safety Center (NESC) Systems Engineering (SE) Technical Discipline Team (TDT) initiated the Model Based Systems Engineering (MBSE) Pathfinder effort in FY16. The goals and objectives of the MBSE Pathfinder include developing and advancing MBSE capability across NASA, applying MBSE to real NASA issues, and capturing issues and opportunities surrounding MBSE. The Pathfinder effort consisted of four teams, with each team addressing a particular focus area. This paper focuses on Pathfinder team 1 with the focus area of architectures and mission campaigns. These efforts covered the timeframe of February 2016 through September 2016. The team was comprised of eight team members from seven NASA Centers (Glenn Research Center, Langley Research Center, Ames Research Center, Goddard Space Flight Center IV&V Facility, Johnson Space Center, Marshall Space Flight Center, and Stennis Space Center). Collectively, the team had varying levels of knowledge, skills and expertise in systems engineering and MBSE. The team applied their existing and newly acquired system modeling knowledge and expertise to develop modeling products for a campaign (Program) of crew and cargo missions (Projects) to establish a human presence on Mars utilizing In-Situ Resource Utilization (ISRU). Pathfinder team 1 developed a subset of modeling products that are required for a Program System Requirement Review (SRR)/System Design Review (SDR) and Project Mission Concept Review (MCR)/SRR as defined in NASA Procedural Requirements. Additionally, Team 1 was able to perform and demonstrate some trades and constraint analyses. At the end of these efforts, over twenty lessons learned and recommended next steps have been identified.

Interactions of UNC-34 Enabled With Rac GTPases and the NIK Kinase MIG-15 in Caenorhabditis elegans Axon Pathfinding and Neuronal Migration

PubMed Central

Shakir, M. Afaq; Gill, Jason S.; Lundquist, Erik A.

2006-01-01

Many genes that affect axon pathfinding and cell migration have been identified. Mechanisms by which these genes and the molecules they encode interact with one another in pathways and networks to control developmental events are unclear. Rac GTPases, the cytoskeletal signaling molecule Enabled, and NIK kinase have all been implicated in regulating axon pathfinding and cell migration. Here we present evidence that, in Caenorhabditis elegans, three Rac GTPases, CED-10, RAC-2, and MIG-2, define three redundant pathways that each control axon pathfinding, and that the NIK kinase MIG-15 acts in each Rac pathway. Furthermore, we show that the Enabled molecule UNC-34 defines a fourth partially redundant pathway that acts in parallel to Rac/MIG-15 signaling in axon pathfinding. Enabled and the three Racs also act redundantly to mediate AQR and PQR neuronal cell migration. The Racs and UNC-34 Ena might all control the formation of actin-based protrusive structures (lamellipodia and filopodia) that mediate growth cone outgrowth and cell migration. MIG-15 does not act with the three Racs in execution of cell migration. Rather, MIG-15 affects direction of PQR neuronal migration, similar to UNC-40 and DPY-19, which control initial Q cell polarity, and Wnt signaling, which acts later to control Q cell-directed migration. MIG-2 Rac, which acts with CED-10 Rac, RAC-2 Rac, and UNC-34 Ena in axon pathfinding and cell migration, also acts with MIG-15 in PQR directional migration. PMID:16204220

IMP mission

NASA Technical Reports Server (NTRS)

1972-01-01

The program requirements and operations requirements for the IMP mission are presented. The satellite configuration is described and the missions are analyzed. The support equipment, logistics, range facilities, and responsibilities of the launching organizations are defined. The systems for telemetry, communications, satellite tracking, and satellite control are identified.

Disturbance Reduction System Thrusters Stabilize LISA Pathfinder

NASA Image and Video Library

2015-12-03

The LISA Pathfinder spacecraft is on its way to space, having successfully launched from Kourou, French Guiana Dec. 3, 2015. On board is the state-of-the-art Disturbance Reduction System DRS, a thruster technology developed at NASA JPL.

Dark Energy and Gravity Experiment Explorer and Pathfinder

NASA Astrophysics Data System (ADS)

Chiow, S.-w.; Yu, N.

2018-02-01

We propose to utilize the unique gravity and vacuum environment in the orbits of the Deep Space Gateway for direct detections of dark energy using atom interferometers, and for pathfinder experiments for future gravitational wave and dark matter detections.

Two-gigawatt burst-mode operation of the intense microwave prototype (IMP) free-electron laser (FEL) for the microwave tokamak experiment (MTX)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Felker, B.; Allen, S.; Bell, H.

1993-10-06

The MTX explored the plasma heating effects of 140 GHz microwaves from both Gyrotrons and from the IMP FEL wiggler. The Gyrotron was long pulse length (0.5 seconds maximum) and the FEL produced short-pulse length, high-peak power, single and burst modes of 140 GHZ microwaves. Full-power operations of the IMP FEL wiggler were commenced in April of 1992 and continued into October of 1992. The Experimental Test Accelerator H (ETA-II) provided a 50-nanosecond, 6-MeV, 2--3 kAmp electron beam that was introduced co-linear into the IMP FEL with a 140 GHz Gyrotron master oscillator (MO). The FEL was able to amplifymore » the MO signal from approximately 7 kW to peaks consistently in the range of 1--2 GW. This microwave pulse was transmitted into the MTX and allowed the exploration of the linear and non-linear effects of short pulse, intense power in the MTX plasma. Single pulses were used to explore and gain operating experience in the parameter space of the IMP FEL, and finally evaluate transmission and absorption in the MTX. Single-pulse operations were repeatable. After the MTX was shut down burst-mode operations were successful at 2 kHz. This paper will describe the IMP FEL, Microwave Transmission System to MTX, the diagnostics used for calorimetric measurements, and the operations of the entire Microwave system. A discussion of correlated and uncorrelated errors that affect FEL performance will be made Linear and non-linear absorption data of the microwaves in the MTX plasma will be presented.« less

Molecular analysis of the integrons of metallo-β-lactamase-producing Pseudomonas aeruginosa isolates collected by nationwide surveillance programs across Japan.

PubMed

Mano, Yoko; Saga, Tomoo; Ishii, Yoshikazu; Yoshizumi, Ayumi; Bonomo, Robert A; Yamaguchi, Keizo; Tateda, Kazuhiro

2015-02-21

We investigate the evolving molecular epidemiology of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa isolates collected in a 100 institution, nationwide surveillance study in Japan from 2004 to 2006. MBL-producers were detected in 23/996 isolates (2.3%) in 2004 and 21/992 (2.1%) in 2006. Antimicrobial resistance (specifically, carbapenem resistance) rates between two periods did not differ significantly. MBL-producers were more prevalent in urinary tract isolates. bla IMP-1 group was the most predominant (38 isolates, 80%), followed by 3 bla IMP-7, 2 bla IMP-11 group, and 1 bla VIM-1. All MBL genes were identified in 16 different class 1 integrons, most of which were novel to INTEGRALL database. A total of 17 isolates of sequence type (ST) 235, a recognized worldwide drug-resistant lineage, were distributed in 5 geographic regions across Japan. ST235 isolates included a sublineage associated with In113-like integron. ST357 was identified in 14 isolates, 9 of which harboring a sole bla IMP-1 gene cassette (In994) were recovered from Chugoku region in 2004. ST357 isolates with bla IMP-11 group or ST235 with bla IMP-7 emerged in 2006. We also report for the first time the presence of novel fosI gene cassette in strains other than Mycobacterium spp. Our data give an important "snapshot" of the molecular characteristics and dynamics of MBL-producing lineages in P. aeruginosa in Japan. The significant association of specific genotypes and integrons implies that dissemination and transmission of the preexisting resistant lineage, rather than horizontal gene transfer in situ, might largely explain their endemicity.

Synthesis and characterization of aluminosilicate catalyst impregnated by nickel oxide

NASA Astrophysics Data System (ADS)

Maulida, Iffana Dani; Sriatun, Taslimah

2015-09-01

Aluminosilicate as a catalyst has been synthesized by pore-engineering using CetylTrimethylAmmonium-Bromide (CTAB) as templating agent. It can produce bigger aluminosilicate pore therefore it will be more suitable for bulky molecule. The aims of this research are to synthesize aluminosilicate supported by Nickel, using CTAB surfactant as templating agent for larger pore radius than natural zeolite and characterize the synthesis product, consist of total acid sites and surface area characteristic. This research has been done with following steps. First, making sodium silicate and sodium aluminate. Second, aluminosilicate was synthesized by direct methods, calcined at 550, 650 and 750°C variation temperature, characterized product by X-RD and FTIR spectrometer. Third, NiCl2 was impregnated to the aluminosilicate that has the best cristallinity and main TO4 functional groups product (550 sample). Variation of NiCl2:aluminosilicate (w/w) ratio were 25%:75%, 50%:50% and 75%:25%. Last but not least characterization of catalytic properties was performed. It comprised total acidity test (gravimetric method) and Surface Area Analyzer. The result shows that the product synthesized by direct method at 550oC calcination temperature has the best cristallinity and main functional groups of TO4. The highest total acid sites was 31.6 mmole/g (Imp-A sample). Surface Area Analyzer shows that Imp-B sample has the best pore distribution and highest total pore volume and specific surface area with value 32.424 cc/g and 46.8287 m2/g respectively. We can draw the conclusion that the most potential catalyst is Imp-A sample compared to Imp-B and Imp-C because it has the highest total acid sites. However the most effective catalyst used for product selectivity was Imp-B sample among all samples.

Identification of urinary metabolites of imperatorin with a single run on an LC/Triple TOF system based on multiple mass defect filter data acquisition and multiple data mining techniques.

PubMed

Qiao, Shi; Shi, Xiaowei; Shi, Rui; Liu, Man; Liu, Ting; Zhang, Kerong; Wang, Qiao; Yao, Meicun; Zhang, Lantong

2013-08-01

The detection of drug metabolites, especially for minor metabolites, continues to be a challenge because of the complexity of biological samples. Imperatorin (IMP) is an active natural furocoumarin component originating from many traditional Chinese herbal medicines and is expected to be pursued as a new vasorelaxant agent. In the present study, a generic and efficient approach was developed for the in vivo screening and identification of IMP metabolites using liquid chromatography-Triple TOF mass spectrometry. In this approach, a novel on-line data acquisition method mutiple mass defect filter (MMDF) combined with dynamic background subtraction was developed to trace all probable urinary metabolites of IMP. Comparing with the traditionally intensity-dependent data acquisition method, MMDF method could give the information of low-level metabolites masked by background noise and endogenous components. Thus, the minor metabolites in complex biological matrices could be detected. Then, the sensitive and specific multiple data-mining techniques extracted ion chromatography, mass defect filter, product ion filter, and neutral loss filter were used for the discovery of IMP metabolites. Based on the proposed strategy, 44 phase I and 7 phase II metabolites were identified in rat urine after oral administration of IMP. The results indicated that oxidization was the main metabolic pathway and that different oxidized substituent positions had a significant influence on the fragmentation of the metabolites. Two types of characteristic ions at m/z 203 and 219 can be observed in the MS/MS spectra. This is the first study of IMP metabolism in vivo. The interpretation of the MS/MS spectra of these metabolites and the proposed metabolite pathway provide essential data for further pharmacological studies of other linear-type furocoumarins.

Functional expression of the GABAA receptor α2 and α3 subunits at synapses between intercalated medial paracapsular neurons of mouse amygdala

PubMed Central

Geracitano, Raffaella; Fischer, David; Kasugai, Yu; Ferraguti, Francesco; Capogna, Marco

2012-01-01

In the amygdala, GABAergic neurons in the intercalated medial paracapsular cluster (Imp) have been suggested to play a key role in fear learning and extinction. These neurons project to the central (CE) amygdaloid nucleus and to other areas within and outside the amygdala. In addition, they give rise to local collaterals that innervate other neurons in the Imp. Several drugs, including benzodiazepines (BZ), are allosteric modulators of GABAA receptors. BZ has both anxiolytic and sedative actions, which are mediated through GABAA receptors containing α2/α3 and α1 subunits, respectively. To establish whether α1 or α2/α3 subunits are expressed at Imp cell synapses, we used paired recordings of anatomically identified Imp neurons and high resolution immunocytochemistry in the mouse. We observed that a selective α3 subunit agonist, TP003 (100 nM), significantly increased the decay time constant of the unitary IPSCs. A similar effect was also induced by zolpidem (10 μM) or by diazepam (1 μM). In contrast, lower doses of zolpidem (0.1–1 μM) did not significantly alter the kinetics of the unitary IPSCs. Accordingly, immunocytochemical experiments established that the α2 and α3, but not the α1 subunits of the GABAA receptors, were present at Imp cell synapses of the mouse amygdala. These results define, for the first time, some of the functional GABAA receptor subunits expressed at synapses of Imp cells. The data also provide an additional rationale to prompt the search of GABAA receptor α3 selective ligands as improved anxiolytic drugs. PMID:22666188

The impact of assumptions regarding vaccine-induced immunity on the public health and cost-effectiveness of hepatitis A vaccination: Is one dose sufficient?

PubMed

Curran, Desmond; de Ridder, Marc; Van Effelterre, Thierry

2016-11-01

Hepatitis A vaccination stimulates memory cells to produce an anamnestic response. In this study, we used a mathematical model to examine how long-term immune memory might convey additional protection against clinical/icteric infections. Dynamic and decision models were used to estimate the expected number of cases, and the costs and quality-adjusted life-years (QALYs), respectively. Several scenarios were explored by assuming: (1) varying duration of vaccine-induced immune memory, (2) and/or varying levels of vaccine-induced immune memory protection (IMP), (3) and/or varying levels of infectiousness in vaccinated individuals with IMP. The base case analysis assumed a time horizon of 25 y (2012 - 2036), with additional analyses over 50 and 75 y. The analyses were conducted in the Mexican public health system perspective. In the base case that assumed no vaccine-induced IMP, the 2-dose hepatitis A vaccination strategy was cost-effective compared with the 1-dose strategy over the 3 time horizons. However, it was not cost-effective if we assumed additional IMP durations of at least 10 y in the 25-y horizon. In the 50- and 75-y horizons, the 2-dose strategy was always cost-effective, except when 100% reduction in the probability of icteric Infections, 75% reduction in infectiousness, and mean durations of IMP of at least 50 y were assumed. This analysis indicates that routine vaccination of toddlers against hepatitis A virus would be cost-effective in Mexico using a single-dose vaccination strategy. However, the cost-effectiveness of a second dose depends on the assumptions of additional protection by IMP and the time horizon over which the analysis is performed.

The impact of assumptions regarding vaccine-induced immunity on the public health and cost-effectiveness of hepatitis A vaccination: Is one dose sufficient?

PubMed Central

Curran, Desmond; de Ridder, Marc; Van Effelterre, Thierry

2016-01-01

ABSTRACT Hepatitis A vaccination stimulates memory cells to produce an anamnestic response. In this study, we used a mathematical model to examine how long-term immune memory might convey additional protection against clinical/icteric infections. Dynamic and decision models were used to estimate the expected number of cases, and the costs and quality-adjusted life-years (QALYs), respectively. Several scenarios were explored by assuming: (1) varying duration of vaccine-induced immune memory, (2) and/or varying levels of vaccine-induced immune memory protection (IMP), (3) and/or varying levels of infectiousness in vaccinated individuals with IMP. The base case analysis assumed a time horizon of 25 y (2012 – 2036), with additional analyses over 50 and 75 y. The analyses were conducted in the Mexican public health system perspective. In the base case that assumed no vaccine-induced IMP, the 2-dose hepatitis A vaccination strategy was cost-effective compared with the 1-dose strategy over the 3 time horizons. However, it was not cost-effective if we assumed additional IMP durations of at least 10 y in the 25-y horizon. In the 50- and 75-y horizons, the 2-dose strategy was always cost-effective, except when 100% reduction in the probability of icteric Infections, 75% reduction in infectiousness, and mean durations of IMP of at least 50 y were assumed. This analysis indicates that routine vaccination of toddlers against hepatitis A virus would be cost-effective in Mexico using a single-dose vaccination strategy. However, the cost-effectiveness of a second dose depends on the assumptions of additional protection by IMP and the time horizon over which the analysis is performed. PMID:27428611

Nuclear transport adapts to varying heat stress in a multistep mechanism.

PubMed

Ogawa, Yutaka; Imamoto, Naoko

2018-05-10

Appropriate cell growth conditions are limited to a narrow temperature range. Once the temperature is out of this range, cells respond to protect themselves, but temperature thresholds at which various intracellular responses occur, including nuclear transport systems, remain unclear. Using a newly developed precise temperature shift assay, we found that individual transport pathways have different sensitivities to a rise in temperature. Nuclear translocations of molecular chaperone HSP70s occur at a much lower temperature than the inhibition of Ran-dependent transport. Subsequently, importin (Imp) α/β-dependent import ceases at a lower temperature than other Ran-dependent transport, suggesting that these are controlled by independent mechanisms. In vitro research revealed that the inhibition of Imp α/β-dependent import is caused by the dysfunction of Imp α1 specifically at lower temperature. Thus, the thermosensitivity of Imp α1 modulates transport balances and enables the multistep shutdown of Ran-dependent transport systems according to the degree of heat stress. © 2018 Ogawa and Imamoto.

Report on final recommendations for IMPS engineering-science payload

NASA Technical Reports Server (NTRS)

Garrett, H. B.

1984-01-01

Six general categories of key scientific and engineering concerns for the interactions measurements payload for shuttle (IMPS) mission are addressed: (1) dielectric charging; (2) material property changes; (3) electromagnetic interference, plasma interactions, and plasma wake effects associated with high-voltage solar arrays and large space structures; (4) radio frequency distortion and nonlinearities due to the enhanced plasma in the shuttle ram/wake; (5) shuttle glow and contamination; and (6) plasma interactions with the space-based radar. Lesser concerns are the interactions associated with EVA; the radiation and SEU effects peculiar to the auroral/polar cap environments; and space debris. The measurements needed to address the concerns associated with the general categories are described and a list of generic investigations capable of making the required measurements, emphasizing the spectrum of measurements necessary to quantize the interactions in the auroral/polar environments are included. A suggested ground-test plan for the IMPS project, a description of proposed follow-on IMPS missions, and a detailed bibliography for each of the interactions discussed are included.

Insulin-like growth factor II messenger RNA-binding protein-3 is an independent prognostic factor in uterine leiomyosarcoma.

PubMed

Yasutake, Nobuko; Ohishi, Yoshihiro; Taguchi, Kenichi; Hiraki, Yuka; Oya, Masafumi; Oshiro, Yumi; Mine, Mari; Iwasaki, Takeshi; Yamamoto, Hidetaka; Kohashi, Kenichi; Sonoda, Kenzo; Kato, Kiyoko; Oda, Yoshinao

2018-04-01

The aim of this study was to identify the prognostic factors of uterine leiomyosarcoma (ULMS). We reviewed 60 cases of surgically resected ULMSs and investigated conventional clinicopathological factors, together with the expression of insulin-like growth factor II messenger RNA-binding protein-3 (IMP3), hormone receptors and cell cycle regulatory markers by immunohistochemistry. Mediator complex subunit 12 (MED12) mutation analysis was also performed. Univariate analyses revealed that advanced stage (P < 0.0001), older age (P = 0.0244) and IMP3 expression (P = 0.0011) were significant predictors of a poor outcome. Multivariate analysis revealed advanced stage (P < 0.0001) and IMP3 (P = 0.0373) as independent predictors of a poor prognosis. Expressions of cell cycle markers and hormone receptors, and MED12 mutations (12% in ULMSs) were not identified as prognostic markers in this study. IMP3 expression in ULMS could be a marker of a poor prognosis. © 2017 John Wiley & Sons Ltd.

Modis, SeaWIFS, and Pathfinder funded activities

NASA Technical Reports Server (NTRS)

Evans, Robert H.

1995-01-01

MODIS (Moderate Resolution Imaging Spectrometer), SeaWIFS (Sea-viewing Wide Field Sensor), Pathfinder, and DSP (Digital Signal Processor) objectives are summarized. An overview of current progress is given for the automatic processing database, client/server status, matchup database, and DSP support.

Design Overview of the DM Radio Pathfinder Experiment

NASA Technical Reports Server (NTRS)

Silva-Feaver, Maximiliano; Chaudhuri, Saptarshi; Cho, Hsaio-Mei; Dawson, Carl; Graham, Peter; Irwin, Kent; Kuenstner, Stephen; Li, Dale; Mardon, Jeremy; Moseley, Harvey;

Strategy for selecting Mars Pathfinder landing sites

NASA Technical Reports Server (NTRS)

Greeley, Ronald; Kuzmin, Ruslin O.

1994-01-01

A strategy for Pathfinder site selection must be developed that is fundamentally different from most previous considerations. At least two approaches can be identified. In one approach, the objective is to select a site representing a key geologic unit on Mars, i.e., a unit that is widespread, easily recognized, and used frequently as a datum in various investigations. The second approach is to select a site that potentially affords access to a wide variety of rock types. Because rover range is limited, rocks from a variety of sources must be assembled in a small area for sampling. Regardless of the approach taken in site selection, the Pathfinder site should include eolian deposits and provisions should be made to obtain measurements on soils. A recommended approach for selecting the Mars Pathfinder landing site is to identify a deltaic deposit, composed of sediments derived from sources of various ages and geologic units that shows evidence of eolian activity. The site should be located as close as possible to the part of the outwash where rapid deposition occurred because the likelihood of 'sorting' by size and composition increases with distance, decreasing the probability of heterogeneity. In addition, it is recommended that field operation tests be conducted to gain experience and insight into conducting science with Pathfinder.

Muscle Microvascular Blood Flow, Oxygenation, pH, and Perfusion Pressure Decrease in Simulated Acute Compartment Syndrome.

PubMed

Challa, Sravya T; Hargens, Alan R; Uzosike, Amarachi; Macias, Brandon R

2017-09-06

The current gold standard for diagnosing acute compartment syndrome (ACS) is an assessment of clinical signs, invasive measurement of intramuscular pressure (IMP), and measurement of local perfusion pressure. However, IMP measurements have several shortcomings, including pain, risk of infection, risk of technique error, plugging of the catheter tip, lack of consensus on the diagnostic pressure threshold, and lack of specificity and sensitivity. The objective of this study was to evaluate muscle hemodynamics, oxygenation, and pH as diagnostic parameters in a human model of ACS. We hypothesized that as IMP increases, muscle microvascular blood flow, oxygenation, and pH decrease in the anterior compartment of a leg at heart level and that they decrease significantly more when the leg is elevated further. An external pneumatic leg pressure chamber, combined with a venous stasis thigh cuff, was used to increase IMP and simulate ACS. Eight healthy subjects (5 males and 3 females; mean age, 26 years) had photoplethysmography and near-infrared spectroscopy-pH sensors placed over the middle aspect of the tibialis anterior muscle of the right (experimental) and left (control) legs. Leg chamber pressure conditions (40, 50, and 60 mm Hg) were applied in a randomized order after baseline measurements were taken. Data were collected continuously for each 11-minute pressure condition, with an 11-minute recovery period after each condition, and the average of the last 6 minutes was used for data analyses. The same protocol was repeated with each subject's legs elevated 12 cm above heart level. Data were analyzed using repeated-measures analysis of variance (ANOVA). As IMP increased, muscle microvascular blood flow (p = 0.01), oxygenation (p < 0.001), and pH (p < 0.001) all decreased significantly in the experimental leg compared with the control leg. At all IMP levels, leg elevation significantly decreased muscle oxygenation (p = 0.013) and perfusion pressure (p = 0.03) compared with the control leg at heart level. These results indicate that muscle microvascular blood flow, oxygenation, pH, and perfusion pressure decrease significantly as IMP increases in a human model of ACS. This study identifies hemodynamic and metabolic parameters as potential noninvasive diagnostic tools for ACS.

Status of the laser ion source at IMP

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sha, S.; Graduate University of Chinese Academy of Sciences, Beijing 100049; School of Nuclear science and technology, Lanzhou University, Lanzhou 73000

2012-02-15

A laser (Nd:YAG laser, 3 J, 1064 nm, 8-10 ns) ion source has been built and under development at IMP to provide pulsed high-charge-state heavy ion beams to a radio frequency quadrupole (RFQ) for upgrading the IMP accelerators with a new low-energy beam injector. The laser ion source currently operates in a direct plasma injection scheme to inject the high charge state ions produced from a solid target into the RFQ. The maximum power density on the target was about 8.4 x 10{sup 12} W/cm{sup 2}. The preliminary experimental results will be presented and discussed in this paper.

Circular Probable Error for Circular and Noncircular Gaussian Impacts

DTIC Science & Technology

2012-09-01

1M simulated impacts ph(k)=mean(imp(:,1).^2+imp(:,2).^2<=CEP^2); % hit frequency on CEP end phit (j)=mean(ph...avg 100 hit frequencies to “incr n” end % GRAPHICS plot(i, phit ,’r-’); % error exponent versus Ph estimate

The Consequences of a Problem-Based Mathematics Curriculum

ERIC Educational Resources Information Center

Clarke, David; Breed, Margarita; Fraser, Sherry

2004-01-01

Implementation of a problem-based mathematics curriculum, the "Interactive Mathematics Program" (IMP), at three high schools in California has been associated with more than just differences in student achievement. The outcomes that distinguished students who participated in the IMP program from students who followed a conventional…

Detection of VIM-2-, IMP-1- and NDM-1-producing multidrug resistant Pseudomonas aeruginosa in Malaysia.

PubMed

Liew, Siew Mun; Rajasekaram, Ganeswrei; Puthucheary, Savithri D; Chua, Kek Heng

2018-02-09

The increasing incidence of carbapenem-resistant Pseudomonas aeruginosa along with the discovery of novel metallo-β-lactamases (MBLs) is of concern. In this study, the isolation of Malaysian MBL-producing P. aeruginosa clinical strains was investigated. Fifty-three P. aeruginosa clinical strains were isolated from different patients in Sultanah Aminah Hospital, Johor Bahru, Malaysia in 2015. Antimicrobial susceptibility test was conducted. Minimum inhibitory concentrations (MICs) of imipenem and meropenem were determined by Etest. The carbapenem-resistant strains were screened for MBL production by IMP-EDTA double disk synergy test (DDST), MBL imipenem/imipenem-inhibitor (IP/IPI) Etest and polymerase chain reaction (PCR). Genotyping was performed by multilocus sequence typing (MLST) analysis. Three (5.7%) clinical strains were identified as MBL producers. Multidrug resistance was observed in the three strains, and two were resistant to all the antimicrobials tested. Sequencing analysis confirmed the three strains to harbour carbapenemase genes: one with bla IMP-1 , one with bla VIM-2 and the other with bla NDM-1 genes. These multidrug resistant strains were identified as sequence type (ST) 235 and ST308. None of the bla IMP-1 and bla NDM-1 genes have been reported in Malaysian P. aeruginosa. The emergence of imipenemase 1 (IMP-1)- and New Delhi metallo-β-lactamase 1 (NDM-1)-producing P. aeruginosa in Malaysia maybe travel-associated. Copyright © 2018. Published by Elsevier Ltd.

A Comprehensive Framework for International Medical Programs: A 2017 consensus statement from the American College of Academic International Medicine

PubMed Central

Garg, Manish; Peck, Gregory L.; Arquilla, Bonnie; Miller, Andrew C.; Soghoian, Sari E.; Anderson III, Harry L.; Bloem, Christina; Firstenberg, Michael S.; Galwankar, Sagar C.; Guo, Weidun Alan; Izurieta, Ricardo; Krebs, Elizabeth; Hansoti, Bhakti; Nanda, Sudip; Nwachuku, Chinenye O.; Nwomeh, Benedict; Paladino, Lorenzo; Papadimos, Thomas J.; Sharpe, Richard P.; Swaroop, Mamta; Stawicki, Stanislaw P.

2017-01-01

The American College of Academic International Medicine (ACAIM) represents a group of clinicians who seek to promote clinical, educational, and scientific collaboration in the area of Academic International Medicine (AIM) to address health care disparities and improve patient care and outcomes globally. Significant health care delivery and quality gaps persist between high-income countries (HICs) and low-and-middle-income countries (LMICs). International Medical Programs (IMPs) are an important mechanism for addressing these inequalities. IMPs are international partnerships that primarily use education and training-based interventions to build sustainable clinical capacity. Within this overall context, a comprehensive framework for IMPs (CFIMPs) is needed to assist HICs and LMICs navigate the development of IMPs. The aim of this consensus statement is to highlight best practices and engage the global community in ACAIM's mission. Through this work, we highlight key aspects of IMPs including: (1) the structure; (2) core principles for successful and ethical development; (3) information technology; (4) medical education and training; (5) research and scientific investigation; and (6) program durability. The ultimate goal of current initiatives is to create a foundation upon which ACAIM and other organizations can begin to formalize a truly global network of clinical education/training and care delivery sites, with long-term sustainability as the primary pillar of international inter-institutional collaborations. PMID:29291171

A novel facile method of labeling octreotide with (18)F-fluorine.

PubMed

Laverman, Peter; McBride, William J; Sharkey, Robert M; Eek, Annemarie; Joosten, Lieke; Oyen, Wim J G; Goldenberg, David M; Boerman, Otto C

2010-03-01

Several methods have been developed to label peptides with (18)F. However, in general these are laborious and require a multistep synthesis. We present a facile method based on the chelation of (18)F-aluminum fluoride (Al(18)F) by 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA). The method is characterized by the labeling of NOTA-octreotide (NOTA-d-Phe-cyclo[Cys-Phe-d-Trp-Lys-Thr-Cys]-Throl (MH(+) 1305) [IMP466]) with (18)F. Octreotide was conjugated with the NOTA chelate and labeled with (18)F in a 2-step, 1-pot method. The labeling procedure was optimized with regard to the labeling buffer, peptide, and aluminum concentration. Radiochemical yield, specific activity, in vitro stability, and receptor affinity were determined. Biodistribution of (18)F-IMP466 was studied in AR42J tumor-bearing mice and compared with that of (68)Ga-labeled IMP466. In addition, small-animal PET/CT images were acquired. IMP466 was labeled with Al(18)F in a single step with 50% yield. The labeled product was purified by high-performance liquid chromatography to remove unbound Al(18)F and unlabeled peptide. The radiolabeling, including purification, was performed in 45 min. The specific activity was 45,000 GBq/mmol, and the peptide was stable in serum for 4 h at 37 degrees C. Labeling was performed at pH 4.1 in sodium citrate, sodium acetate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, and 2-(N-morpholino)ethanesulfonic acid buffer and was optimal in sodium acetate buffer. The apparent 50% inhibitory concentration of the (19)F-labeled IMP466 determined on AR42J cells was 3.6 nM. Biodistribution studies at 2 h after injection showed a high tumor uptake of (18)F-IMP466 (28.3 +/- 5.2 percentage injected dose per gram [%ID/g]; tumor-to-blood ratio, 300 +/- 90), which could be blocked by an excess of unlabeled peptide (8.6 +/- 0.7 %ID/g), indicating that the accumulation in the tumor was receptor-mediated. Biodistribution of (68)Ga-IMP466 was similar to that of (18)F-IMP466. (18)F-IMP466 was stable in vivo, because bone uptake was only 0.4 +/- 0.2 %ID/g, whereas free Al(18)F accumulated rapidly in the bone (36.9 +/- 5.0 %ID/g at 2 h after injection). Small-animal PET/CT scans showed excellent tumor delineation and high preferential accumulation in the tumor. NOTA-octreotide could be labeled rapidly and efficiently with (18)F using a 2-step, 1-pot method. The compound was stable in vivo and showed rapid accretion in somatostatin receptor subtype 2-expressing AR42J tumors in nude mice. This method can be used to label other NOTA-conjugated compounds with (18)F.

Baseline Design and Performance Analysis of Laser Altimeter for Korean Lunar Orbiter

NASA Astrophysics Data System (ADS)

Lim, Hyung-Chul; Neumann, Gregory A.; Choi, Myeong-Hwan; Yu, Sung-Yeol; Bang, Seong-Cheol; Ka, Neung-Hyun; Park, Jong-Uk; Choi, Man-Soo; Park, Eunseo

2016-09-01

Korea’s lunar exploration project includes the launching of an orbiter, a lander (including a rover), and an experimental orbiter (referred to as a lunar pathfinder). Laser altimeters have played an important scientific role in lunar, planetary, and asteroid exploration missions since their first use in 1971 onboard the Apollo 15 mission to the Moon. In this study, a laser altimeter was proposed as a scientific instrument for the Korean lunar orbiter, which will be launched by 2020, to study the global topography of the surface of the Moon and its gravitational field and to support other payloads such as a terrain mapping camera or spectral imager. This study presents the baseline design and performance model for the proposed laser altimeter. Additionally, the study discusses the expected performance based on numerical simulation results. The simulation results indicate that the design of system parameters satisfies performance requirements with respect to detection probability and range error even under unfavorable conditions.

Characterization of the Eimeria maxima sporozoite surface protein IMP1

USDA-ARS?s Scientific Manuscript database

The purpose of this study was to characterize Eimeria maxima immunoprotective protein IMP1 that is hypothesized to play a role in eliciting protective immunity against E. maxima infection in chickens. RT-PCR analysis of RNA from unsporulated and sporulating E. maxima oocysts revealed highest transc...

Membrane interactions between secretion granules and plasmalemma in three exocrine glands

PubMed Central

Tanaka, Y; De Camilli, P; Meldolesi, J

1980-01-01

Three types of membrane interactions were studied in three exocrine systems (the acinar cells of the rat parotid, rat lacrimal gland, and guinea pig pancrease) by freeze- fracture and thin-section electron microscopy: exocytosis, induced in vivo by specific pharmacological stimulations; the mutual apposition of secretory granule membranes in the intact cell; membrane appositions induced in vitro by centrifugation of the isolated granules. In all three glandular cells, the distribution of intramembrane particles (IMP) on the fracture faces of the luminal plasmagranule membrane particles (IMP) on the fracture faces of the lumenal plasmalemma appeared random before stimulation. However, after injection of secretagogues, IMP were rapidly clearly from the areas of granule- plasmalemma apposition in the parotid cells and, especially, in lacrimocytes. In the latter, the cleared areas appeared as large bulges toward the lumen, whereas in the parotid they were less pronounced. Exocytotic openings were usually large and the fracture faces of their rims were covered with IMP. In contrast, in stimulated pancreatic acinar cells, the IMP distribution remained apparently random after stimulation. Exocytoses were established through the formation of narrown necks, and no images which might correspond to early stages of membrane fusion were revealed. Within the cytoplasm of parotid and lacrimal cells (but not in the pancreas), both at rest and after stimulation, secretion granules were often closely apposed by means of flat, circular areas, also devoid of IMP. In thin sections, the images corresponding to IMP-free areas were close granule-granule and granule-plasmalemma appositions, sometimes with focal merging of the membrane outer layers to yield pentalaminar structures. Isolated secretion granules were forced together in vitro by centrifugation. Under these conditions, increasing the centrifugal force from 1,600 to 50,000 g for 10 min resulted in a progressive, statistically significant increase of the frequency of IMP-free flat appositions between parotid granules. In contrast, no such areas were seen between freeze-fractured pancreatic granules, although some focal pentalaminar appositions appeared in section after centrifugation at 50 and 100,000 g for 10 min. On the basis of the observation that, in secretory cells, IMP clearing always develops in deformed membrane areas (bulges, depressions, flat areas), it is suggested that it might result from the forced mechanical apposition of the interacting membranes. This might be a preliminary process not sufficient to initiate fusion. In the pancreas, IMP clearing could occur over surface areas too small to be detected. In stimulated parotid and lacrimal glands they were exceptional. These structures were either attached at the sites of continuity between granule and plasma membranes, or free in the acinar lumen, with a preferential location within exocytotic pockets or in their proximity. Experiments designed to investigate the nature of these blisters and vesicles revealed that they probably arise artifactually during glutaraldehyde fixation. In fact, (a) they were large and numerous in poorly fixed samples but were never observed in thin sections of specimens fixed in one step with glutaraldehyde and OsO(4); and (b) no increase in concentration of phospholipids was observed in the parotid saliva and pancreatic juice after stimulation of protein discharge, as was to be expected if release of membrane material were occurring after exocytosis. PMID:7380885

Curricular/Instructional Technology Resources.

ERIC Educational Resources Information Center

Roy, Loriene, Comp.

Part of a larger report on the Four Directions Project, an American Indian technology innovation project, this section includes 10 "pathfinders" to locating information on learning and instructional technology resources. The pathfinders were designed by students in the Graduate School of Library and Information Science at the University…

Internet Technology Resources.

ERIC Educational Resources Information Center

Roy, Loriene, Comp.

Part of a larger report on the Four Directions Project, an American Indian technology innovation project, this section includes six "pathfinders" to locating information on Internet resources. The pathfinders were designed by students in the Graduate School of Library and Information Science at the University of Texas at Austin in…

Cultural Themes.

ERIC Educational Resources Information Center

Roy, Loriene, Comp.

Part of a larger report on the Four Directions Project, an American Indian technology innovation project, this section includes 10 "pathfinders" to locating information on Native American cultural themes. The pathfinders were designed by students in the Graduate School of Library and Information Science at the University of Texas at…

Correspondence and Least Squares Analyses of Soil and Rock Compositions for the Viking Lander 1 and Pathfinder Sites

NASA Technical Reports Server (NTRS)

Larsen, K. W.; Arvidson, R. E.; Jolliff, B. L.; Clark, B. C.

2000-01-01

Correspondence and Least Squares Mixing Analysis techniques are applied to the chemical composition of Viking 1 soils and Pathfinder rocks and soils. Implications for the parent composition of local and global materials are discussed.

Atmosphere Processing Module Automation and Catalyst Durability Analysis for Mars ISRU Pathfinder

NASA Technical Reports Server (NTRS)

Petersen, Elspeth M.

2016-01-01

The Mars In-Situ Resource Utilization Pathfinder was designed to create fuel using components found in the planet’s atmosphere and regolith for an ascension vehicle to return a potential sample return or crew return vehicle from Mars. The Atmosphere Processing Module (APM), a subunit of the pathfinder, uses cryocoolers to isolate and collect carbon dioxide from Mars simulant gas. The carbon dioxide is fed with hydrogen into a Sabatier reactor where methane is produced. The APM is currently undergoing the final stages of testing at Kennedy Space Center prior to process integration testing with the other subunits of the pathfinder. The automation software for the APM cryocoolers was tested and found to perform nominally. The catalyst used for the Sabatier reactor was investigated to determine the factors contributing to catalyst failure. The results from the catalyst testing require further analysis, but it appears that the rapid change in temperature during reactor start up or the elevated operating temperature is responsible for the changes observed in the catalyst.

Distributed Active Archive Center

NASA Technical Reports Server (NTRS)

Bodden, Lee; Pease, Phil; Bedet, Jean-Jacques; Rosen, Wayne

1993-01-01

The Goddard Space Flight Center Version 0 Distributed Active Archive Center (GSFC V0 DAAC) is being developed to enhance and improve scientific research and productivity by consolidating access to remote sensor earth science data in the pre-EOS time frame. In cooperation with scientists from the science labs at GSFC, other NASA facilities, universities, and other government agencies, the DAAC will support data acquisition, validation, archive and distribution. The DAAC is being developed in response to EOSDIS Project Functional Requirements as well as from requirements originating from individual science projects such as SeaWiFS, Meteor3/TOMS2, AVHRR Pathfinder, TOVS Pathfinder, and UARS. The GSFC V0 DAAC has begun operational support for the AVHRR Pathfinder (as of April, 1993), TOVS Pathfinder (as of July, 1993) and the UARS (September, 1993) Projects, and is preparing to provide operational support for SeaWiFS (August, 1994) data. The GSFC V0 DAAC has also incorporated the existing data, services, and functionality of the DAAC/Climate, DAAC/Land, and the Coastal Zone Color Scanner (CZCS) Systems.

PATHFINDER: Probing Atmospheric Flows in an Integrated and Distributed Environment

NASA Technical Reports Server (NTRS)

Wilhelmson, R. B.; Wojtowicz, D. P.; Shaw, C.; Hagedorn, J.; Koch, S.

1995-01-01

PATHFINDER is a software effort to create a flexible, modular, collaborative, and distributed environment for studying atmospheric, astrophysical, and other fluid flows in the evolving networked metacomputer environment of the 1990s. It uses existing software, such as HDF (Hierarchical Data Format), DTM (Data Transfer Mechanism), GEMPAK (General Meteorological Package), AVS, SGI Explorer, and Inventor to provide the researcher with the ability to harness the latest in desktop to teraflop computing. Software modules developed during the project are available in the public domain via anonymous FTP from the National Center for Supercomputing Applications (NCSA). The address is ftp.ncsa.uiuc.edu, and the directory is /SGI/PATHFINDER.

Pathfinder: A Retrospective

NASA Technical Reports Server (NTRS)

Landis, Geoffrey A.; Lyons, Valerie (Technical Monitor)

2002-01-01

Mars is one of the most interesting planets in the solar system, featuring enormous canyons, giant volcanoes, and indications that, early in its history, it might have had rivers and perhaps even oceans. Five years ago, in July of 1997, the Pathfinder mission landed on Mars, bringing with it the microwave-oven sized Sojourner rover to wander around on the surface and analyse rocks. Among the experiments on the mission was one designed to analyse dust deposition. Pathfinder is only the first of an armada of spacecraft which will examine Mars from the pole to the equator in the next decade, culminating with a mission to bring humans to Mars.

Holographic beam mapping of the CHIME pathfinder array

NASA Astrophysics Data System (ADS)

Berger, Philippe; Newburgh, Laura B.; Amiri, Mandana; Bandura, Kevin; Cliche, Jean-François; Connor, Liam; Deng, Meiling; Denman, Nolan; Dobbs, Matt; Fandino, Mateus; Gilbert, Adam J.; Good, Deborah; Halpern, Mark; Hanna, David; Hincks, Adam D.; Hinshaw, Gary; Höfer, Carolin; Johnson, Andre M.; Landecker, Tom L.; Masui, Kiyoshi W.; Mena Parra, Juan; Oppermann, Niels; Pen, Ue-Li; Peterson, Jeffrey B.; Recnik, Andre; Robishaw, Timothy; Shaw, J. Richard; Siegel, Seth; Sigurdson, Kris; Smith, Kendrick; Storer, Emilie; Tretyakov, Ian; Van Gassen, Kwinten; Vanderlinde, Keith; Wiebe, Donald

2016-08-01

The Canadian Hydrogen Intensity Mapping Experiment (CHIME) Pathfinder radio telescope is currently surveying the northern hemisphere between 400 and 800 MHz. By mapping the large scale structure of neutral hydrogen through its redshifted 21 cm line emission between z 0.8-2.5 CHIME will contribute to our understanding of Dark Energy. Bright astrophysical foregrounds must be separated from the neutral hydrogen signal, a task which requires precise characterization of the polarized telescope beams. Using the DRAO John A. Galt 26 m telescope, we have developed a holography instrument and technique for mapping the CHIME Pathfinder beams. We report the status of the instrument and initial results of this effort.

Pathfinder, v6 n6, Nov/Dec 2008. Foundation Data and Technology

DTIC Science & Technology

2008-12-01

Geospatial-Intelligence Agency,Office of Corporate Communications,4600 Sangamore Road ,Bethesda,MD, 20816 -5003 8. PERFORMING ORGANIZATION REPORT...Communications 4600 Sangamore Road, Mail Stop D-54 Bethesda, MD 20816 -5003 Telephone: (301) 227-7388, DSN 287-7388 E-mail: pathfinder@nga.mil

A Pathfinder for Animal Research and Animal Rights.

ERIC Educational Resources Information Center

Anderson, David C.

1992-01-01

This pathfinder was originally prepared for "Biomedical Research and Animal Rights," a session sponsored by the Veterinary Medical Libraries and Research Libraries Sections of the Medical Library Association. Current resources are described, from bibliographies to electronic bulletin boards, which relate to the issue of laboratory animal…

Is it feasible to pool funds for local children's services in England? Evidence from the national evaluation of children's trust pathfinders.

PubMed

Lorgelly, Paula; Bachmann, Max; Shreeve, Ann; Reading, Richard; Thorburn, June; Mugford, Miranda; O'Brien, Margaret; Husbands, Chris

2009-01-01

To describe how funds were pooled or otherwise jointly managed by National Health Service (NHS) primary care trusts and local authorities in England. To compare expenditure on local children's services by health, education and social services. We conducted a questionnaire survey of all 35 children's trust pathfinders, six months after they were launched, with a follow-up at 2.5 years. We also undertook an in-depth analysis of local authorities and primary care trusts, within eight pathfinder areas and three non-pathfinder areas, whereby we compared expenditure on children's services, interviewed managers and professionals and examined financial documents. Local authorities and NHS trusts coordinated expenditure in various ways, most commonly through informal agreements and aligning budgets but also by formally pooling budgets. The latter were usually for selected services such as child and adolescent mental health services, though four children's trusts pathfinders pooled (or aligned) their budgets for all children's services. Total expenditure per child was greatest for education, lowest for social services and intermediate for health. However, it was difficult to quantify education expenditure on children with health and social care needs, and health care expenditure on children. Sharing money for local children's services requires shared objectives, trust, and legal and accounting expertise. Several different mechanisms are permitted and many are feasible but programme budgeting for children's services could make them more effective.

Uncoupling nicotine mediated motoneuron axonal pathfinding errors and muscle degeneration in zebrafish

DOE Office of Scientific and Technical Information (OSTI.GOV)

Welsh, Lillian; Tanguay, Robert L.; Svoboda, Kurt R.

Zebrafish embryos offer a unique opportunity to investigate the mechanisms by which nicotine exposure impacts early vertebrate development. Embryos exposed to nicotine become functionally paralyzed by 42 hpf suggesting that the neuromuscular system is compromised in exposed embryos. We previously demonstrated that secondary spinal motoneurons in nicotine-exposed embryos were delayed in development and that their axons made pathfinding errors (Svoboda, K.R., Vijayaraghaven, S., Tanguay, R.L., 2002. Nicotinic receptors mediate changes in spinal motoneuron development and axonal pathfinding in embryonic zebrafish exposed to nicotine. J. Neurosci. 22, 10731-10741). In that study, we did not consider the potential role that altered skeletalmore » muscle development caused by nicotine exposure could play in contributing to the errors in spinal motoneuron axon pathfinding. In this study, we show that an alteration in skeletal muscle development occurs in tandem with alterations in spinal motoneuron development upon exposure to nicotine. The alteration in the muscle involves the binding of nicotine to the muscle-specific AChRs. The nicotine-induced alteration in muscle development does not occur in the zebrafish mutant (sofa potato, [sop]), which lacks muscle-specific AChRs. Even though muscle development is unaffected by nicotine exposure in sop mutants, motoneuron axonal pathfinding errors still occur in these mutants, indicating a direct effect of nicotine exposure on nervous system development.« less

Basic principles for measurement of intramuscular pressure

NASA Technical Reports Server (NTRS)

Hargens, A. R.; Ballard, R. E.

1995-01-01

We review historical and methodological approaches to measurements of intramuscular pressure (IMP) in humans. These techniques provide valuable measures of muscle tone and activity as well as diagnostic criteria for evaluation of exertional compartment syndrome. Although the wick and catheter techniques provide accurate measurements of IMP at rest, their value for exercise studies and diagnosis of exertional compartment syndrome is limited because of low frequency response and hydrostatic (static and inertial) pressure artifacts. Presently, most information on diagnosis of exertional compartment syndromes during dynamic exercise is available using the Myopress catheter. However, future research and clinical diagnosis using IMP can be optimized by the use of a miniature transducer-tipped catheter such as the Millar Mikro-tip.

Acceleration mechanisms for energetic particles in the earth's magnetosphere

NASA Technical Reports Server (NTRS)

Schiferl, S.; Fan, C. Y.; Hsieh, K. C.; Erickson, K. N.; Gloeckler, G.

1982-01-01

By analyzing data on energetic particle fluxes measured simultaneously with detector systems on several earth satellites, signatures of different acceleration mechanisms for these particles were searched for. One of the samples is an event observed on ATS-6 and IMP-7. IMP-7 was in the dusk quarter at 38 earth radii while ATS-6 was located at local midnight at a distance of 6.6 earth radii. Although the flux variations as observed on the two spacecraft both showed 1.5 min periodicity, there was a 40-second time lag with IMP-7 behind. The results indicate that the particles are accelerated by magnetic field line annihilation, with the x-point located at about 10 earth radii.

Reduction and analysis of data from the plasma wave instruments on the IMP-6 and IMP-8 spacecraft

NASA Technical Reports Server (NTRS)

Gurnett, D. A.; Anderson, R. R.

1983-01-01

The primary data reduction effort during the reporting period was to process summary plots of the IMP 8 plasma wave data and to submit these data to the National Space Science Data Center. Features of the electrostatic noise are compared with simultaneous observations of the magnetic field, plasma and energetic electrons. Spectral characteristics of the noise and the results of this comparison both suggest that in its high frequency part at least the noise does not belong to normal modes of plasma waves but represents either quasi-thermal noise in the non-Maxwellian plasma or artificial noise generated by spacecraft interaction with the medium.

A New Era in Solar Thermal-IR Astronomy: the NSO Array Camera (NAC) on the McMath-Pierce Telescope

NASA Astrophysics Data System (ADS)

Ayres, T.; Penn, M.; Plymate, C.; Keller, C.

2008-09-01

The U.S. National Solar Observatory Array Camera (NAC) is a cryogenically cooled 1Kx1K InSb ``Aladdin" array that recently became operational at the McMath-Pierce facility on Kitt Peak, a high dry site in the southwest U.S. (Arizona). The new camera is similar to those already incorporated into instruments on nighttime telescopes, and has unprecedented sensitivity, low noise, and excellent cosmetics compared with the Amber Engineering (AE) device it replaces. (The latter was scavenged from a commercial surveillance camera in the 1990's: only 256X256 format, high noise, and annoying flatfield structure). The NAC focal plane is maintained at 30 K by a mechanical closed-cycle helium cooler, dispensing with the cumbersome pumped--solid-N2 40 K system used previously with the AE camera. The NAC linearity has been verified for exposures as short as 1 ms, although latency in the data recording holds the maximum frame rate to about 8 Hz (in "streaming mode"). The camera is run in tandem with the Infrared Adaptive Optics (IRAO) system. Utilizing a 37-actuator deformable mirror, IRAO can--under moderate seeing conditions--correct the telescope image to the diffraction limit longward of 2.3 mu (if a suitable high contrast target is available: the IR granulation has proven too bland to reliably track). IRAO also provides fine control over the solar image for spatial scanning in long-slit mode with the 14 m vertical "Main" spectrograph (MS). A 1'X1' area scan, with 0.5" steps orthogonal to the slit direction, requires less than half a minute, much shorter than p-mode and granulation evolution time scales. A recent engineering test run, in April 2008, utilized NAC/IRAO/MS to capture the fundamental (4.6 mu) and first-overtone (2.3 mu) rovibrational bands of CO, including maps of quiet regions, drift scans along the equatorial limbs (to measure the off-limb molecular emissions), and imaging of a fortuitous small sunspot pair, a final gasp, perhaps, of Cycle 23. Future work with the NAC will emphasize pathfinding toward the next generation of IR imaging spectrometers for the Advanced Technology Solar Telescope, whose 4 m aperture finally will bring sorely needed high spatial resolution to daytime infrared astronomy. In the meantime, the NAC is available to qualified solar physicists from around the world to conduct forefront research in the 1-5 mu region, on the venerable--but infrared friendly--McMath-Pierce telescope.

IMP I, H, and J

NASA Technical Reports Server (NTRS)

1973-01-01

The schedule for the IMP project for the eighth, ninth, and tenth satellites of the series is presented. A description of the spacecraft and the weekly summaries of the operations performed on the spacecraft are provided. The project planning, project problems, recommendations, and reports of launch operations are described. Drawings of the satellite structures are included.

XM746 Practice Fuze.

DTIC Science & Technology

1980-01-31

SANDMILLISEC HARD SAND .. : 33 20!~ U SMOKE EXIT RELATIONSHIP pd TO XMBO04 PROJ SURIAL DEPTH AS A FUNCTION OF EXIT HOLE LOCATION IMP VEL - 900 PT/SIC IMP...distance between the be determined. Adenes evlope for then obser- 30% ordinate and 16% or 84% The 0confidence level" d- Ttons was derived as follows

Interpretation Training in Individuals with Generalized Social Anxiety Disorder: A Randomized Controlled Trial

ERIC Educational Resources Information Center

Amir, Nader; Taylor, Charles T.

2012-01-01

Objective: To examine the efficacy of a multisession computerized interpretation modification program (IMP) in the treatment of generalized social anxiety disorder (GSAD). Method: The sample comprised 49 individuals meeting diagnostic criteria for GSAD who were enrolled in a randomized, double-blind placebo-controlled trial comparing IMP (n = 23)…

[Examination of metallo-beta-lactamase-producing different types of Serratia marcescens detected in the same patient].

PubMed

Takamitsu, Ito; Fukui, Yasuo; Ono, Noriaki; Ikeda, Fumiaki; Kanayama, Akiko; Kobayashi, Intetsu

2013-03-01

Metallo-beta-lactamase (MBL) producing Serratia marcescens isolate was recovered from a study patient in September, 2007 in whom MBL non-producing S. marcescens had been isolated 2 months previously. Two S. marcescens isolates recovered from the study patient showed the same pulsed-field gel electrophoresis (PFGE) pattern. Seven S. marcescens isolates were recovered from other patients in our hospital during August, 2007 and November, 2007. Five of the seven isolates produced MBL. All of the MBL-producing isolates showed the same PFGE pattern and harbored plasmids of the same size and bla(IMP) genes. The bla(IMP) genes were easily transferred to Escherichia coli DH5alpha by transformation of a plasmid purified from the MBL-producing isolate. Those transformation experiments suggested that bla(IMP) genes were encoded by the plasmid. From these observations, it was speculated that the MBL non-producing S. marcescens isolate recovered from the study patient had acquired the plasmid which encoded bla(IMP) genes and a monoclone of MBL-producing S. marcescens spread horizontally in our hospital.

The Pathfinder Microrover

NASA Technical Reports Server (NTRS)

Matijevic, J. R.; Bickler, D. B.; Braun, D. F.; Eisen, H. J.; Matthies, L. H.; Mishkin, A. H.; Stone, H. W.; van Nieuwstadt, L. M.; Wen, L. C.; Wilcox, B. H.;

Pathfinders: An Intellectual Guide to Libraries.

ERIC Educational Resources Information Center

Jung, Claudia Ruediger; And Others

Intended as an example for other college libraries, this collection of 38 pathfinders and bibliographies was developed by the reference staff of the Calvin Coolidge Library at Castleton State College, Vermont. Designed to present the types of literature available in particular subject fields and those works readily available in the Coolidge…

Pathfinder Teaching and Learning Units.

ERIC Educational Resources Information Center

Hawaii Univ., Honolulu. Sea Grant Program.

This collection of teaching units were selected from materials developed during the Operation Pathfinder Institutes (OPI) which took place in the Pacific region between 1994 and 1999. The institutes were intended to provide upper elementary and middle school science teachers with an opportunity to develop a deeper understanding of the marine…

Sedimentary geomorphology of the Mars Pathfinder Landing Site

NASA Technical Reports Server (NTRS)

Rice, James W., Jr.; Parker, Timothy Jay

1997-01-01

The first landing on Mars in over 20 years will take place July 4, 1997, near te mouth of the Ares Vallis outflow channel located in southeastern Chryse Planitia. Mars Pathfinder, unlike Viking 1, is expected to land on a surface that has a distinct and unambiguous fluvial signature.

Teacher Job Satisfaction: Lessons from the TSW Pathfinder Project

ERIC Educational Resources Information Center

Butt, Graham; Lance, Ann; Fielding, Antony; Gunter, Helen; Rayner, Steve; Thomas, Hywel

2005-01-01

Government policy assumes that modernization and remodelling will be effective as external intervention mechanisms to improve job satisfaction. Based on data collected as part of the evaluation of the "Transforming the School Workforce Pathfinder Project", an argument is presented here which suggests that internal management models may…

Airbag Retraction

NASA Image and Video Library

1997-07-05

This image shows that the Mars Pathfinder airbags have been successfully retracted, allowing safe deployment of the rover ramps. The Sojourner rover is at lower right, and rocks are visible in the background. Mars Pathfinder landed successfully on the surface of Mars today at 10:07 a.m. PDT. http://photojournal.jpl.nasa.gov/catalog/PIA00618

Pathfinders on Black Dance in America.

ERIC Educational Resources Information Center

Roy, Loriene, Ed.

This is a compilation of 18 pathfinders (i.e., a bibliographic instruction aid) on black dance in America, prepared by graduate students in the "Information Resources in the Humanities" and the "Information Resources in the Social Sciences" classes in the Graduate School of Library and Information Science at the University of…

Mechanical design of the Mars Pathfinder mission

NASA Technical Reports Server (NTRS)

Eisen, Howard Jay; Buck, Carl W.; Gillis-Smith, Greg R.; Umland, Jeffrey W.

1997-01-01

The Mars Pathfinder mission and the Sojourner rover is reported on, with emphasis on the various mission steps and the performance of the technologies involved. The mechanical design of mission hardware was critical to the success of the entry sequence and the landing operations. The various mechanisms employed are considered.

Pathfinder, Volume 7. Number 4, Jul/Aug 2009. GEOINT in Action

DTIC Science & Technology

2009-08-01

Rd,Bethesda,MD, 20816 -5003 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S...Communications 4600 Sangamore Road, Mail Stop D-39 Bethesda, MD 20816 -5003 Telephone: (301) 227-7388, DSN 287-7388 E-mail: pathfinder@nga.mil

Pathfinder, v6 n3, May/Jun 2008. Unifying the Intelligence Profession

DTIC Science & Technology

2008-06-01

ADDRESS(ES) National Geospatial-Intelligence Agency,Office of Corporate Communications,4600 Sangamore Road ,Bethesda,MD, 20816 -5003 8. PERFORMING...Sangamore Road, Mail Stop D-54 Bethesda, MD 20816 -5003 Telephone: (301) 227-7388, DSN 287-7388 E-mail: pathfinder@nga.mil Director Vice Adm. Robert

Pathfinder, v6 n5, Sep/Oct 2008. Shielding Our Home and Nation

DTIC Science & Technology

2008-10-01

Agency,Office of Corporate Communications,4600 Sangamore Road ,Bethesda,MD, 20816 -5003 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING... 20816 -5003 Telephone: (301) 227-7388, DSN 287-7388 E-mail: pathfinder@nga.mil Director Vice Adm. Robert B. Murrett, U.S. Navy Deputy Director

Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination

PubMed Central

Chen, Shuqing; Zeng, Baosheng; James, Anthony A.; Tan, Anjiang; Huang, Yongping

2017-01-01

Manipulation of sex determination pathways in insects provides the basis for a wide spectrum of strategies to benefit agriculture and public health. Furthermore, insects display a remarkable diversity in the genetic pathways that lead to sex differentiation. The silkworm, Bombyx mori, has been cultivated by humans as a beneficial insect for over two millennia, and more recently as a model system for studying lepidopteran genetics and development. Previous studies have identified the B. mori Fem piRNA as the primary female determining factor and BmMasc as its downstream target, while the genetic scenario for male sex determination was still unclear. In the current study, we exploite the transgenic CRISPR/Cas9 system to generate a comprehensive set of knockout mutations in genes BmSxl, Bmtra2, BmImp, BmImpM, BmPSI and BmMasc, to investigate their roles in silkworm sex determination. Absence of Bmtra2 results in the complete depletion of Bmdsx transcripts, which is the conserved downstream factor in the sex determination pathway, and induces embryonic lethality. Loss of BmImp or BmImpM function does not affect the sexual differentiation. Mutations in BmPSI and BmMasc genes affect the splicing of Bmdsx and the female reproductive apparatus appeared in the male external genital. Intriguingly, we identify that BmPSI regulates expression of BmMasc, BmImpM and Bmdsx, supporting the conclusion that it acts as a key auxiliary factor in silkworm male sex determination. PMID:28103247

Intramuscular pressures beneath elastic and inelastic leggings

NASA Technical Reports Server (NTRS)

Murthy, G.; Ballard, R. E.; Breit, G. A.; Watenpaugh, D. E.; Hargens, A. R.

1994-01-01

Leg compression devices have been used extensively by patients to combat chronic venous insufficiency and by astronauts to counteract orthostatic intolerance following spaceflight. However, the effects of elastic and inelastic leggings on the calf muscle pump have not been compared. The purpose of this study was to compare in normal subjects the effects of elastic and inelastic compression on leg intramuscular pressure (IMP), an objective index of calf muscle pump function. IMP in soleus and tibialis anterior muscles was measured with transducer-tipped catheters. Surface compression between each legging and the skin was recorded with an air bladder. Subjects were studied under three conditions: (1) control (no legging), (2) elastic legging, and (3) inelastic legging. Pressure data were recorded for each condition during recumbency, sitting, standing, walking, and running. Elastic leggings applied significantly greater surface compression during recumbency (20 +/- 1 mm Hg, mean +/- SE) than inelastic leggings (13 +/- 2 mm Hg). During recumbency, elastic leggings produced significantly higher soleus IMP of 25 +/- 1 mm Hg and tibialis anterior IMP of 28 +/- 1 mm Hg compared to 17 +/- 1 mm Hg and 20 +/- 2 mm Hg, respectively, generated by inelastic leggings and 8 +/- 1 mm Hg and 11 +/- 1 mm Hg, respectively, without leggings. During sitting, walking, and running, however, peak IMPs generated in the muscular compartments by elastic and inelastic leggings were similar. Our results suggest that elastic leg compression applied over a long period in the recumbent posture may impede microcirculation and jeopardize tissue viability.(ABSTRACT TRUNCATED AT 250 WORDS).

Symptoms, endoscopic findings and reflux monitoring results in candidates for bariatric surgery.

PubMed

Heimgartner, Benjamin; Herzig, Marcus; Borbély, Yves; Kröll, Dino; Nett, Philipp; Tutuian, Radu

2017-07-01

Gastroesophageal reflux disease (GERD) is common in patients with obesity. Diagnosing GERD is important as bariatric operations have different influence on GERD. We assessed reflux symptoms and objective findings prior to surgery. Work-up included esophageal symptoms quantification by VAS-scores, esophagogastroduodenoscopy (EGD) and 24-h impedance-pH (imp-pH) monitoring off PPI therapy. Imp-pH was classified as abnormal if either %time pH<4 was abnormal, total number of reflux episodes was elevated or symptom index (SI) was positive. Among 100 consecutive patients (68F, age 40±11years, BMI 44.9±6.9kg/m 2 ) 54% reported heartburn and/or regurgitation, 71% had objective evidence of GERD (38% endoscopic lesions and 33% only abnormal imp-pH results). Imp-pH was superior to EGD in identifying GERD (sensitivity 85% vs. 54%, p<0.01). Symptomatic and asymptomatic patients had similar prevalence of esophageal lesions (37% vs. 39%) and abnormal imp-pH findings (68% vs. 50%). Sixty nine percent of patients with abnormal %time pH<4 had a normal number of reflux episodes. Half of patients with obesity reported typical GERD symptoms and >70% had evidence of GERD. Poor acid clearance was the main mechanisms. Since typical reflux symptoms don't predict objective findings, endoscopy and reflux monitoring should be part of the surgery work-up especially before restrictive procedures. Copyright © 2017 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

Pathophysiologic study of chronic infarcts with I-123 isopropyl iodo-amphetamine (IMP): the importance of periinfarct area

DOE Office of Scientific and Technical Information (OSTI.GOV)

Raynaud, C.; Rancurel, G.; Samson, Y.

1987-01-01

Seventeen chronic cerebral infarcts were investigated by a highly sensitive, dedicated brain single photon emission computerized tomography system using /sup 123/I-isopropyl iodoamphetamine (IMP) and /sup 133/Xe. IMP uptake was measured 10 minutes, 2 hours, and 5 hours after injection, and regional cerebral blood flow was measured with 133Xe. In 4 cases a positron emission tomography system was used to measure the rCBF and the regional metabolic rate of oxygen with C15O2 and 15O2. The results obtained allowed us to identify 2 abnormal zones. One, the central area, was characterized by a severe decrease in IMP uptake and rCBF averaging 34%more » and 46% respectively and by a hypodense image on the x-ray computerized tomography scan. The second, the periinfarct or ''peripheral area'' was characterized by a moderate decrease in IMP uptake and regional cerebral blood flow averaging 13 and 19% respectively; this area extended around the central area and had a normal density on computerized tomography scan. The IMP hypofixation of the peripheral area observed at the 10th minute tended to disappear at the 5th hour. The volume of this area was often found to be quite large, covering more than 30% of a hemisphere whereas the central area did not exceed 25%. Volume appeared to be correlated with the neurological status of the patient. The nature of the peripheral area is not established with certainty. It may be caused by deafferentation of areas not directly affected by the ischemic insult and/or selective ischemic neuronal loss. The results stress the important role played by the peripheral area, which may be useful in establishing the prognosis and evaluating the efficacy of therapy in individual stroke cases.« less

Study on Enhancement Principle and Stabilization for the Luminol-H2O2-HRP Chemiluminescence System

PubMed Central

Yang, Lihua; Jin, Maojun; Du, Pengfei; Chen, Ge; Zhang, Chan; Wang, Jian; Jin, Fen; Shao, Hua; She, Yongxin; Wang, Shanshan; Zheng, Lufei; Wang, Jing

2015-01-01

A luminol-H2O2-HRP chemiluminescence system with high relative luminescent intensity (RLU) and long stabilization time was investigated. First, the comparative study on the enhancement effect of ten compounds as enhancers to the luminol-H2O2-HRP chemiluminescence system was carried out, and the results showed that 4-(imidazol-1-yl)phenol (4-IMP), 4-iodophenol (4-IOP), 4-bromophenol (4-BOP) and 4-hydroxy-4’-iodobiphenyl (HIOP) had the best performance. Based on the experiment, the four enhancers were dissolved in acetone, acetonitrile, methanol, and dimethylformamide (DMF) with various concentrations, the results indicated that 4-IMP, 4-IOP, 4-BOP and HIOP dissolved in DMF with the concentrations of 0.2%, 3.2%, 1.6% and 3.2% could get the highest RLU values. Subsequently, the influences of pH, ionic strength, HRP, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol on the stabilization of the luminol-H2O2-HRP chemiluminescence system were studied, and we found that pH value, ionic strength, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol have little influence on luminescent stabilization, while HRP has a great influence. In different ranges of HRP concentration, different enhancers should be selected. When the concentration is within the range of 0~6 ng/mL, 4-IMP should be selected. When the concentration of HRP ranges from 6 to 25ng/mL, 4-IOP was the best choice. And when the concentration is within the range of 25~80 ng/mL, HIOP should be selected as the enhancer. Finally, the three well-performing chemiluminescent enhanced solutions (CESs) have been further optimized according to the three enhancers (4-IMP, 4-IOP and HIOP) in their utilized HRP concentration ranges. PMID:26154162

Multiyear, Multinational Survey of the Incidence and Global Distribution of Metallo-β-Lactamase-Producing Enterobacteriaceae and Pseudomonas aeruginosa.

PubMed

Kazmierczak, Krystyna M; Rabine, Sharon; Hackel, Meredith; McLaughlin, Robert E; Biedenbach, Douglas J; Bouchillon, Samuel K; Sahm, Daniel F; Bradford, Patricia A

2016-02-01

Metallo-β-lactamases (MBLs) hydrolyze all classes of β-lactams except monobactams and are not inhibited by classic serine β-lactamase inhibitors. Gram-negative pathogens isolated from patient infections were collected from 202 medical centers in 40 countries as part of a global surveillance study from 2012 to 2014. Carbapenem-nonsusceptible Enterobacteriaceae and Pseudomonas aeruginosa were characterized for bla genes encoding VIM, IMP, NDM, SPM, and GIM variants using PCR and sequencing. A total of 471 MBL-positive isolates included the following species (numbers of isolates are in parentheses): P. aeruginosa (308), Klebsiella spp. (85), Enterobacter spp. (39), Proteeae (16), Citrobacter freundii (12), Escherichia coli (6), and Serratia marcescens (5) and were submitted by sites from 34 countries. Of these, 69.6% were collected in 9 countries (numbers of isolates are in parentheses): Russia (72), Greece (61), Philippines (54), Venezuela (29), and Kuwait, Nigeria, Romania, South Africa, and Thailand (20 to 25 isolates each). Thirty-two different MBL variants were detected (14 VIM, 14 IMP, and 4 NDM enzymes). Seven novel MBL variants were encountered in the study, each differing from a previously reported variant by one amino acid substitution: VIM-42 (VIM-1 [V223I]), VIM-43 (VIM-4 [A24V]), VIM-44 (VIM-2 [K257N]), VIM-45 (VIM-2 [T35I]), IMP-48 (IMP-14 [I69T]), IMP-49 (IMP-18 [V49F]), and NDM-16 (NDM-1 [R264H]). The in vitro activities of all tested antibiotics against MBL-positive Enterobacteriaceae were significantly reduced with the exception of that of aztreonam-avibactam (MIC90, 0.5 to 1 μg/ml), whereas colistin was the most effective agent against MBL-positive P. aeruginosa isolates (>97% susceptible). Although the global percentage of isolates encoding MBLs remains relatively low, their detection in 12 species, 34 countries, and all regions participating in this surveillance study is concerning. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Multiyear, Multinational Survey of the Incidence and Global Distribution of Metallo-β-Lactamase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

PubMed Central

Rabine, Sharon; Hackel, Meredith; McLaughlin, Robert E.; Biedenbach, Douglas J.; Bouchillon, Samuel K.; Sahm, Daniel F.; Bradford, Patricia A.

2015-01-01

Metallo-β-lactamases (MBLs) hydrolyze all classes of β-lactams except monobactams and are not inhibited by classic serine β-lactamase inhibitors. Gram-negative pathogens isolated from patient infections were collected from 202 medical centers in 40 countries as part of a global surveillance study from 2012 to 2014. Carbapenem-nonsusceptible Enterobacteriaceae and Pseudomonas aeruginosa were characterized for bla genes encoding VIM, IMP, NDM, SPM, and GIM variants using PCR and sequencing. A total of 471 MBL-positive isolates included the following species (numbers of isolates are in parentheses): P. aeruginosa (308), Klebsiella spp. (85), Enterobacter spp. (39), Proteeae (16), Citrobacter freundii (12), Escherichia coli (6), and Serratia marcescens (5) and were submitted by sites from 34 countries. Of these, 69.6% were collected in 9 countries (numbers of isolates are in parentheses): Russia (72), Greece (61), Philippines (54), Venezuela (29), and Kuwait, Nigeria, Romania, South Africa, and Thailand (20 to 25 isolates each). Thirty-two different MBL variants were detected (14 VIM, 14 IMP, and 4 NDM enzymes). Seven novel MBL variants were encountered in the study, each differing from a previously reported variant by one amino acid substitution: VIM-42 (VIM-1 [V223I]), VIM-43 (VIM-4 [A24V]), VIM-44 (VIM-2 [K257N]), VIM-45 (VIM-2 [T35I]), IMP-48 (IMP-14 [I69T]), IMP-49 (IMP-18 [V49F]), and NDM-16 (NDM-1 [R264H]). The in vitro activities of all tested antibiotics against MBL-positive Enterobacteriaceae were significantly reduced with the exception of that of aztreonam-avibactam (MIC90, 0.5 to 1 μg/ml), whereas colistin was the most effective agent against MBL-positive P. aeruginosa isolates (>97% susceptible). Although the global percentage of isolates encoding MBLs remains relatively low, their detection in 12 species, 34 countries, and all regions participating in this surveillance study is concerning. PMID:26643349

Study on Enhancement Principle and Stabilization for the Luminol-H2O2-HRP Chemiluminescence System.

PubMed

Yang, Lihua; Jin, Maojun; Du, Pengfei; Chen, Ge; Zhang, Chan; Wang, Jian; Jin, Fen; Shao, Hua; She, Yongxin; Wang, Shanshan; Zheng, Lufei; Wang, Jing

2015-01-01

A luminol-H2O2-HRP chemiluminescence system with high relative luminescent intensity (RLU) and long stabilization time was investigated. First, the comparative study on the enhancement effect of ten compounds as enhancers to the luminol-H2O2-HRP chemiluminescence system was carried out, and the results showed that 4-(imidazol-1-yl)phenol (4-IMP), 4-iodophenol (4-IOP), 4-bromophenol (4-BOP) and 4-hydroxy-4'-iodobiphenyl (HIOP) had the best performance. Based on the experiment, the four enhancers were dissolved in acetone, acetonitrile, methanol, and dimethylformamide (DMF) with various concentrations, the results indicated that 4-IMP, 4-IOP, 4-BOP and HIOP dissolved in DMF with the concentrations of 0.2%, 3.2%, 1.6% and 3.2% could get the highest RLU values. Subsequently, the influences of pH, ionic strength, HRP, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol on the stabilization of the luminol-H2O2-HRP chemiluminescence system were studied, and we found that pH value, ionic strength, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol have little influence on luminescent stabilization, while HRP has a great influence. In different ranges of HRP concentration, different enhancers should be selected. When the concentration is within the range of 0~6 ng/mL, 4-IMP should be selected. When the concentration of HRP ranges from 6 to 25 ng/mL, 4-IOP was the best choice. And when the concentration is within the range of 25~80 ng/mL, HIOP should be selected as the enhancer. Finally, the three well-performing chemiluminescent enhanced solutions (CESs) have been further optimized according to the three enhancers (4-IMP, 4-IOP and HIOP) in their utilized HRP concentration ranges.

Carbapenem-Nonsusceptible Enterobacteriaceae in Taiwan

PubMed Central

Wang, Jann-Tay; Wu, Un-In; Lauderdale, Tsai-Ling Yang; Chen, Mei-Chen; Li, Shu-Ying; Hsu, Le-Yin; Chang, Shan-Chwen

2015-01-01

A total of 1135 carbapenem-resistant (nonsusceptible) Enterobacteriaceae (CRE) isolates were recovered between November 2010 and July 2012 (517 from 2010-2011 and 618 from 2012) from 4 hospitals in Taiwan. Carbapenemase-producing Enterobacteriaceae (CPE) comprised 5.0% (57 isolates), including 17 KPC-2 (16 Klebsiella pneumoniae and 1 Escherichia coli), 1 NDM-1 (K. oxytoca), 37 IMP-8 (26 Enterobacter cloacae, 4 Citrobacter freundii, 4 Raoultella planticola, 1 K. pneumoniae, 1 E. coli and 1 K. oxytoca), and 2 VIM-1 (1 E. cloacae, 1 E. coli). The KPC-2-positive K. pneumoniae were highly clonal even in isolates from different hospitals, and all were ST11. IMP-8 positive E. cloacae from the same hospitals showed higher similarity in PFGE pattern than those from different hospitals. A total of 518 CRE isolates (45.6%) were positive for bla ESBL, while 704 (62.0%) isolates were bla AmpC-positive, 382 (33.6% overall) of which carried both bla ESBL and bla AmpC. CTX-M (414, 80.0%) was the most common bla ESBL, while DHA (497, 70.6%) and CMY (157, 22.3%) were the most common bla AmpC. Co-carriage of bla ESBL and bla AmpC was detected in 31 (54.4%) and 15 (26.3%) of the 57 CPE, respectively. KPC-2 was the most common carbapenemase detected in K. pneumoniae (2.8%), while IMP-8 was the most common in E. cloacae (9.7%). All KPC-2-positive CRE were resistant to all three tested carbapenems. However, fourteen of the 37 IMP-8-positive CRE were susceptible to both imipenem and meropenem in vitro. Intra- and inter-hospital spread of KPC-2-producing K. pneumoniae and IMP-8-producing E. cloacae likely occurred. Although the prevalence of CPE is still low, careful monitoring is urgently needed. Non-susceptibility to ertapenem might need to be considered as one criterion of definition for CRE in areas where IMP type carbapenemase is prevalent. PMID:25794144

High prevalence of Salmonella and IMP-4-producing Enterobacteriaceae in the silver gull on Five Islands, Australia.

PubMed

Dolejska, Monika; Masarikova, Martina; Dobiasova, Hana; Jamborova, Ivana; Karpiskova, Renata; Havlicek, Martin; Carlile, Nicholas; Priddel, David; Cizek, Alois; Literak, Ivan

2016-01-01

The objective of this study was to investigate the silver gull as an indicator of environmental contamination by salmonellae and carbapenemase-producing Enterobacteriaceae (CPE) in south-east Australia. A total of 504 cloacal samples were collected from gull chicks at three nesting colonies in New South Wales, Australia [White Bay (n = 144), Five Islands (n = 200) and Montague Island (n = 160)] and were examined for salmonellae and CPE. Isolates were tested for carbapenemase genes and susceptibility to 14 antibiotics. Clonality was determined by PFGE and MLST. Genetic context and conjugative transfer of the carbapenemase gene were determined. A total of 120 CPE of 10 species, mainly Escherichia coli (n = 85), carrying the gene blaIMP-4, blaIMP-38 or blaIMP-26 were obtained from 80 (40%) gulls from Five Islands. Thirty percent of birds from this colony were colonized by salmonellae. Most isolates contained the gene within a class 1 integron showing a blaIMP-4-qacG-aacA4-catB3 array. The blaIMP gene was carried by conjugative plasmids of variable sizes (80-400 kb) and diverse replicons, including HI2-N (n = 30), HI2 (11), A/C (17), A/C-Y (2), L/M (5), I1 (1) and non-typeable (6). Despite the overall high genetic variability, common clones and plasmid types were shared by different birds and bacterial isolates, respectively. Our data demonstrate a large-scale transmission of carbapenemase-producing bacteria into wildlife, likely as a result of the feeding habits of the birds at a local waste depot. The isolates from gulls showed significant similarities with clinical isolates from Australia, suggesting the human origin of the isolates. The sources of CPE for gulls on Five Islands should be explored and proper measures applied to stop the transmission into the environment. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

Mars Orbiter Camera High Resolution Images: Some Results From The First 6 Weeks In Orbit

NASA Technical Reports Server (NTRS)

1997-01-01

The Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) images acquired shortly after orbit insertion were relatively poor in both resolution and image quality. This poor performance was solely the result of low sunlight conditions and the relative distance to the planet, both of which have been progressively improving over the past six weeks. Some of the better images are used here (see PIA01021 through PIA01029) to illustrate how the MOC images provide substantially better views of the martian surface than have ever been recorded previously from orbit.

This U.S. Geological Survey shaded relief map provides an overall context for the MGS MOC images of the Tithonium/Ius Chasma, Ganges Chasma, and Schiaparelli Crater. Closeup images of the Tithonium/Ius Chasma area are visible in PIA01021 through PIA01023. Closeups of Ganges Chasma are available as PIA01027 through PIA01029, and Schiaparelli Crater is shown in PIA01024 through PIA01026. The Mars Pathfinder landing site is shown to the north of the sites of the MGS images.

Launched on November 7, 1996, Mars Global Surveyor entered Mars orbit on Thursday, September 11, 1997. The original mission plan called for using friction with the planet's atmosphere to reduce the orbital energy, leading to a two-year mapping mission from close, circular orbit (beginning in March 1998). Owing to difficulties with one of the two solar panels, aerobraking was suspended in mid-October and resumed in November 8. Many of the original objectives of the mission, and in particular those of the camera, are likely to be accomplished as the mission progresses.

Malin Space Science Systems and the California Institute of Technology built the MOC using spare hardware from the Mars Observer mission. MSSS operates the camera from its facilities in San Diego, CA. The Jet Propulsion Laboratory's Mars Surveyor Operations Project operates the Mars Global Surveyor spacecraft with its industrial partner, Lockheed Martin Astronautics, from facilities in Pasadena, CA and Denver, CO.

The Importance of the Presence of a 5′-Ribonucleotide and the Contribution of the T1R1 + T1R3 Heterodimer and an Additional Low-Affinity Receptor in the Taste Detection of l-Glutamate as Assessed Psychophysically

PubMed Central

Smith, Kimberly R.

2014-01-01

The molecular receptors underlying the purported “umami” taste quality commonly associated with l-glutamate have been controversial. Evidence supports the involvement of the T1R1 + T1R3 heterodimer, a GPCR broadly tuned to l-amino acids, but variants of two mGluRs expressed in taste buds have also been implicated. Using a rigorous psychophysical taste-testing paradigm, we demonstrated impaired, if not eliminated, detection of MSG in WT and T1R1, T1R2, T1R3, and T1R2 + T1R3 KO mice when the contribution of sodium was minimized by the epithelial sodium channel blocker amiloride. When inosine 5′-monophosphate (IMP), a ribonucleotide that potentiates the l-glutamate signal through the T1R1 + T1R3 heterodimer, was added, the WT and T1R2 KO mice were able to detect the compound stimulus across all MSG (+amiloride) concentrations due, in part, to the taste of IMP. In contrast, mice lacking T1R1 or T1R3 could not detect IMP alone, yet some were able to detect MSG + amiloride + IMP, but only at the higher MSG concentrations. Interestingly, the sensitivity of T1R1 KO mice to another l-amino acid, lysine, was unimpaired, suggesting that some l-amino acids can be detected through T1R1 + T1R3-independent receptors without sensitivity loss. Given that IMP is not thought to affect mGluRs, behavioral detection of l-glutamate appears to require the contribution of the T1R1 + T1R3 receptor. However, the partial competence observed in some T1R1 and T1R3 KO mice when MSG + amiloride + IMP was tested suggests that a T1R1 or T1R3 homodimer or an unidentified protein, perhaps in conjunction with T1R1 or T1R3, can serve as a low-affinity taste receptor for l-glutamate in the presence of IMP. PMID:25253867

The importance of the presence of a 5'-ribonucleotide and the contribution of the T1R1 + T1R3 heterodimer and an additional low-affinity receptor in the taste detection of L-glutamate as assessed psychophysically.

PubMed

Smith, Kimberly R; Spector, Alan C

2014-09-24

The molecular receptors underlying the purported "umami" taste quality commonly associated with l-glutamate have been controversial. Evidence supports the involvement of the T1R1 + T1R3 heterodimer, a GPCR broadly tuned to l-amino acids, but variants of two mGluRs expressed in taste buds have also been implicated. Using a rigorous psychophysical taste-testing paradigm, we demonstrated impaired, if not eliminated, detection of MSG in WT and T1R1, T1R2, T1R3, and T1R2 + T1R3 KO mice when the contribution of sodium was minimized by the epithelial sodium channel blocker amiloride. When inosine 5'-monophosphate (IMP), a ribonucleotide that potentiates the l-glutamate signal through the T1R1 + T1R3 heterodimer, was added, the WT and T1R2 KO mice were able to detect the compound stimulus across all MSG (+amiloride) concentrations due, in part, to the taste of IMP. In contrast, mice lacking T1R1 or T1R3 could not detect IMP alone, yet some were able to detect MSG + amiloride + IMP, but only at the higher MSG concentrations. Interestingly, the sensitivity of T1R1 KO mice to another l-amino acid, lysine, was unimpaired, suggesting that some l-amino acids can be detected through T1R1 + T1R3-independent receptors without sensitivity loss. Given that IMP is not thought to affect mGluRs, behavioral detection of l-glutamate appears to require the contribution of the T1R1 + T1R3 receptor. However, the partial competence observed in some T1R1 and T1R3 KO mice when MSG + amiloride + IMP was tested suggests that a T1R1 or T1R3 homodimer or an unidentified protein, perhaps in conjunction with T1R1 or T1R3, can serve as a low-affinity taste receptor for l-glutamate in the presence of IMP. Copyright © 2014 the authors 0270-6474/14/3413234-12$15.00/0.

Interpretation criteria for FDG PET/CT in multiple myeloma (IMPeTUs): final results. IMPeTUs (Italian myeloma criteria for PET USe).

PubMed

Nanni, Cristina; Versari, Annibale; Chauvie, Stephane; Bertone, Elisa; Bianchi, Andrea; Rensi, Marco; Bellò, Marilena; Gallamini, Andrea; Patriarca, Francesca; Gay, Francesca; Gamberi, Barbara; Ghedini, Pietro; Cavo, Michele; Fanti, Stefano; Zamagni, Elena

2018-05-01

ᅟ: FDG PET/CT ( 18 F-fluoro-deoxy-glucose positron emission tomography/computed tomography) is a useful tool to image multiple myeloma (MM). However, simple and reproducible reporting criteria are still lacking and there is the need for harmonization. Recently, a group of Italian nuclear medicine experts defined new visual descriptive criteria (Italian Myeloma criteria for Pet Use: IMPeTUs) to standardize FDG PET/CT evaluation in MM patients. The aim of this study was to assess IMPeTUs reproducibility on a large prospective cohort of MM patients. Patients affected by symptomatic MM who had performed an FDG PET/CT at baseline (PET0), after induction (PET-AI), and the end of treatment (PET-EoT) were prospectively enrolled in a multicenter trial (EMN02)(NCT01910987; MMY3033). After anonymization, PET images were uploaded in the web platform WIDEN® and hence distributed to five expert nuclear medicine reviewers for a blinded independent central review according to the IMPeTUs criteria. Consensus among reviewers was measured by the percentage of agreement and the Krippendorff's alpha. Furthermore, on a patient-based analysis, the concordance among all the reviewers in terms of positivity or negativity of the FDG PET/CT scan was tested for different thresholds of positivity (Deauville score (DS 2, 3, 4, 5) for the main parameters (bone marrow, focal score, extra-medullary disease). Eighty-six patients (211 FDG PET/CT scans) were included in this analysis. Median patient age was 58 years (range, 35-66 years), 45% were male, 15% of them were in stage ISS (International Staging System) III, and 42% had high-risk cytogenetics. The percentage agreement was superior to 75% for all the time points, reaching 100% of agreement in assessing the presence skull lesions after therapy. Comparable results were obtained when the agreement analysis was performed using the Krippendorff's alpha coefficient, either in every single time point of scanning (PET0, PET-AI or PET-EoT) or overall for all the scans together. DS proved highly reproducible with the highest reproducibility for score 4. IMPeTUs criteria proved highly reproducible and could therefore be considered as a base for harmonizing PET interpretation in multiple myeloma. A prospective clinical validation of IMPeTUs criteria is underway.

Interface Generation and Compositional Verification in JavaPathfinder

NASA Technical Reports Server (NTRS)

Giannakopoulou, Dimitra; Pasareanu, Corina

2009-01-01

We present a novel algorithm for interface generation of software components. Given a component, our algorithm uses learning techniques to compute a permissive interface representing legal usage of the component. Unlike our previous work, this algorithm does not require knowledge about the component s environment. Furthermore, in contrast to other related approaches, our algorithm computes permissive interfaces even in the presence of non-determinism in the component. Our algorithm is implemented in the JavaPathfinder model checking framework for UML statechart components. We have also added support for automated assume-guarantee style compositional verification in JavaPathfinder, using component interfaces. We report on the application of the presented approach to the generation of interfaces for flight software components.

Review of the trajectory and atmospheric structure reconstruction for Mars Pathfinder

NASA Astrophysics Data System (ADS)

Withers, Paul; Towner, Martin; Hathi, Brijen; Zarnecki, John

2004-02-01

Mars Pathfinder landed on Mars on July 4, 1997. It used a novel deceleration procedure, consisting of a hypersonic aeroshell, a transonic parachute, retro-rockets, and airbags, to reach the surface safely. Its aerodynamic properties passively maintained a near-zero angle of attack throughout its entry. There were no gyroscopes to monitor attitude. Several different trajectory reconstructions have been based on the assumptions that accelerations along its symmetry axis are directed along its flight path and that accelerations in other directions are insignificant. The aerodynamics of Pathfinder once its parachute opened are still not well-understood and the available observations are probably not sufficient to improve matters significantly in the future.

James Webb Space Telescope Optical Telescope Element Integrated Science Instrument Module (OTIS) Status

NASA Technical Reports Server (NTRS)

Feinberg, Lee; Voyton, Mark; Lander, Julie; Keski-Kuha, Ritva; Matthews, Gary

2016-01-01

The James Webb Space Telescope Optical Telescope Element (OTE) and Integrated ScienceInstrument Module (ISIM)are integrated together to form the OTIS. Once integrated, the OTIS undergoes primary mirrorcenter of curvatureoptical tests, electrical and operational tests, acoustics and vibration testing at the Goddard SpaceFlight Center beforebeing shipped to the Johnson Space Center for cryogenic optical testing of the OTIS. In preparationfor the cryogenicoptical testing, the JWST project has built a Pathfinder telescope and has completed two OpticalGround SystemEquipment (OGSE) cryogenic optical tests with the Pathfinder. In this paper, we will summarize opticaltest results todate and status the final Pathfinder test and the OTIS integration and environmental test preparations

HOWARD EISEN, JPL'S LEAD MECHANICAL TECHNICIAN, HOLDS MARS PATHFINDER 'SOJOURNER' ROVER 1:1 SCALE DU

NASA Technical Reports Server (NTRS)

1996-01-01

The Mars Pathfinder 'Sojourner' rover l:l scale duplicate test vehicle is held by Howard Eisen, its lead mechanical technician from the Jet Propulsion Laboratory, with Kennedy Space Center's Vehicle Assembly Building looming in the background. The launch of NASA's Mars Pathfinder spacecraft aboard a McDonnell Douglas Delta II rocket is scheduled for Monday, Dec. 2, at 2:09:11 a.m. EST. This is a single instantaneous target launch time without a second opportunity on that day. Liftoff will occur from Pad B at Launch Complex 17 on Cape Canaveral Air Station, Fla. There is a 24-day launch opportunity which extends through Dec. 31.

LISA Pathfinder: A Mission Status

NASA Astrophysics Data System (ADS)

Hewitson, Martin; LISA Pathfinder Team Team

2016-03-01

On December 3rd at 04:04 UTC, The European Space Agency launched the LISA Pathfinder satellite on board a VEGA rocket from Kourou in French Guiana. After a series of orbit raising manoeuvres and a 2 month long transfer orbit, LISA Pathfinder arrived at L1. Following a period of commissioning, the science operations commenced at the start of March, beginning the demonstration of technologies and methodologies which pave the way for a future large-scale gravitational wave observatory in space. This talk will present the scientific goals of the mission, discuss the technologies being tested, elucidate the link to a future space-based observatory, such as LISA, and present preliminary results from the in-orbit operations and experiments.

Interplanetary monitoring platform engineering history and achievements

NASA Technical Reports Server (NTRS)

Butler, P. M.

1980-01-01

In the fall of 1979, last of ten Interplanetary Monitoring Platform Satellite (IMP) missions ended a ten year series of flights dedicated to obtaining new knowledge of the radiation effects in outer space and of solar phenomena during a period of maximum solar flare activity. The technological achievements and scientific accomplishments from the IMP program are described.

The discharge of certain amounts of industrial microplastic from a production plant into the River Danube is permitted by the Austrian legislation.

PubMed

Lechner, Aaron; Ramler, David

2015-05-01

Numerous studies have quantified the amount of plastic litter in aquatic ecosystems and tried to assess its impacts and threats. This reflects a rising awareness of plastic as an environmental problem. As a next logical step, identifying and regulating the sources must be in the focus of scientific efforts. We report on a spillage of industrial microplastic (IMP) from a production plant situated at an Austrian Danube tributary. This is the first identified point source of IMP litter in freshwater systems. However, due to generous thresholds established by the Austrian government substantial amounts of IMP are legally introduced into running waters. Copyright © 2015 Elsevier Ltd. All rights reserved.

Substorms, Plasmoids, Flux Robes, and Magnetotail Flux Loss on March 25, 1983: CDAW-8

DTIC Science & Technology

1988-10-03

1400 UT. The magnitude and orientation of the 11 GOES 5 OR 1982-01 9A SE14 14 ZSM -l1ORe ISEE 3 -10 -5 5 OReYSM YS m ISEE 1 5 14r- -* IMP 8 0 Fig. lb...and references therein]. ISEE 1 saw a 31% field decrease from 55 nT to 38 nT between 1020 and 1058 UT, and a 35 % decrease from 62 nT to 41 nT between...strength is determined by the component 29 2.5 POLAR CAP AREA 2.0 E ISEE 1 . 5 -i 60 x - 50 IMP 8 - 40 35 ISEE-3 (B = 22nt) 30 25 ISE IMP8 cc" Z u

[Development Of 25-Year Imp 8 Bow Shock Crossing "List, Ingestion Of This List To Cdaweb, & Enhancement"

NASA Technical Reports Server (NTRS)

Merka, J.; Szabo, A.; Narock, T. W.; King, J. H.; Paularena, K. I.; Richardson, J. D.

2003-01-01

The MIT portion of this project was to use the plasma data from IMP 8 to identify bow shock crossings for construction of a bow shock data base. In collaboration with Goddard, we determined which shock parameters would be included in the catalog and developed a set of flags for characterizing the data. IMP 8 data from 1973-2001 were surveyed for bow shock crossings; the crossings apparent in the plasma data were compared to a list of crossing chosen in the magnetometer data by Goddard. Differences were reconciled to produce a single list. The data were then provided to the NSSDC for archiving. All the work ascribed to MIT in the proposal was completed.

3',5'-cIMP as Potential Second Messenger in the Vascular Wall.

PubMed

Leung, Susan W S; Gao, Yuansheng; Vanhoutte, Paul M

2017-01-01

Traditionally, only the 3',5'-cyclic monophosphates of adenosine and guanosine (produced by adenylyl cyclase and guanylyl cyclase, respectively) are regarded as true "second messengers" in the vascular wall, despite the presence of other cyclic nucleotides in different tissues. Among these noncanonical cyclic nucleotides, inosine 3',5'-cyclic monophosphate (cIMP) is synthesized by soluble guanylyl cyclase in porcine coronary arteries in response to hypoxia, when the enzyme is activated by endothelium-derived nitric oxide. Its production is associated with augmentation of vascular contraction mediated by stimulation of Rho kinase. Based on these findings, cIMP appears to meet most, if not all, of the criteria required for it to be accepted as a "second messenger," at least in the vascular wall.

Pathfinders: Making a Way from Segregation to Community Life.

ERIC Educational Resources Information Center

O'Brien, Connie Lyle; Mount, Beth; O'Brien, John; Rosen, Fredda

This paper describes the Pathfinders program in New York (New York), which works to facilitate the full integration of adults with developmental disabilities into workplaces and neighborhoods. The paper is organized around the question of whether students graduating from special education can find paid and volunteer work in community settings,…

AeroVironment's Jim Daley, Rik Meininger, Derek Lisoski and Wyatt Sadler (clockwise from bottom left) closely monitor systems testing of the Pathfinder-Plus.

NASA Image and Video Library

2004-09-17

AeroVironment's test director Jim Daley, backup pilot Rik Meininger, stability and controls engineer Derek Lisoski and pilot Wyatt Sadler (clockwise from bottom left) closely monitor systems testing of the Pathfinder-Plus solar aircraft from the control station.

Do Integrated Children's Services Improve Children's Outcomes?: Evidence from England's Children's Trust Pathfinders

ERIC Educational Resources Information Center

O'Brien, Margaret; Bachmann, Max O.; Jones, Natalia R.; Reading, Richard; Thoburn, June; Husbands, Chris; Shreeve, Ann; Watson, Jacqueline

2009-01-01

Thirty-five children's trust pathfinders, local cross-sector partnerships, were introduced across England in 2003 to promote greater integration in children's services. Using administrative performance data, this paper tracks yearly trends in child service outputs and child well-being outcomes from 1997 to 2004 in these local areas, including the…

Global Climate Change Pathfinder: A Guide to Information Resources. Second Edition.

ERIC Educational Resources Information Center

Pintozzi, Chestalene; Jones, Douglas E.

This pathfinder is a guide to scientific and technical aspects of global climate change including meteorological and climatological aspects; biological, agricultural, and public policy implications; and the chemical processes involved. Sources are arranged by type of publication and include: (1) 10 reference sources; (2) 12 bibliographies; (3) 44…

Pathfinder. Volume 8, Number 3, May/June 2010. Technology - Rendering an Ever-Clearer Picture

DTIC Science & Technology

2010-06-01

Agency,Office of Corporate Communications,4600 Sangamore Road, Mail Stop D-54,Bethesda,MD, 20816 -5003 8. PERFORMING ORGANIZATION REPORT NUMBER 9...Bethesda, MD 20816 -5003 Telephone: (301) 227-7388, DSN 287-7388 E-mail: pathfinder@nga.mil Director Vice Adm. Robert B. Murrett, U.S. Navy Deputy

Wind Drifts at Viking 1 Landing Site

NASA Technical Reports Server (NTRS)

1997-01-01

This image is of so-called wind drifts seen at the Viking 1 landing site. These are somewhat different from the features seen at the Pathfinder site in two important ways. 1) These landforms have no apparent slip-or avalanche-face as do both terrestrial dunes and the Pathfinder features, and may represent deposits of sediment falling from the air, as opposed to dune sand, which 'hops' or saltates along the ground; 2) these features may indicate erosion on one side, because of the layering and apparent scouring on their right sides. They may, therefore have been deposited by a wind moving left to right, partly or weakly cemented or solidified by surface processes at some later time, then eroded by a second wind (right to left), exposing their internal structure.

Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is a division of the California Institute of Technology (Caltech).

Statistical analysis of soil geochemical data to identify pathfinders associated with mineral deposits: An example from the Coles Hill uranium deposit, Virginia, USA

USGS Publications Warehouse

Levitan, Denise M.; Zipper, Carl E.; Donovan, Patricia; Schreiber, Madeline E.; Seal, Robert; Engle, Mark A.; Chermak, John A.; Bodnar, Robert J.; Johnson, Daniel K.; Aylor, Joseph G.

2015-01-01

Soil geochemical anomalies can be used to identify pathfinders in exploration for ore deposits. In this study, compositional data analysis is used with multivariate statistical methods to analyse soil geochemical data collected from the Coles Hill uranium deposit, Virginia, USA, to identify pathfinders associated with this deposit. Elemental compositions and relationships were compared between the collected Coles Hill soil and reference soil samples extracted from a regional subset of a national-scale geochemical survey. Results show that pathfinders for the Coles Hill deposit include light rare earth elements (La and Ce), which, when normalised by their Al content, are correlated with U/Al, and elevated Th/Al values, which are not correlated with U/Al, supporting decoupling of U from Th during soil generation. These results can be used in genetic and weathering models of the Coles Hill deposit, and can also be applied to future prospecting for similar U deposits in the eastern United States, and in regions with similar geological/climatic conditions.

Actuation crosstalk in free-falling systems: Torsion pendulum results for the engineering model of the LISA pathfinder gravitational reference sensor

NASA Astrophysics Data System (ADS)

Bassan, M.; Cavalleri, A.; De Laurentis, M.; De Marchi, F.; De Rosa, R.; Di Fiore, L.; Dolesi, R.; Finetti, N.; Garufi, F.; Grado, A.; Hueller, M.; Marconi, L.; Milano, L.; Minenkov, Y.; Pucacco, G.; Stanga, R.; Vetrugno, D.; Visco, M.; Vitale, S.; Weber, W. J.

2018-01-01

In this paper we report on measurements on actuation crosstalk, relevant to the gravitational reference sensors for LISA Pathfinder and LISA. In these sensors, a Test Mass (TM) falls freely within a system of electrodes used for readout and control. These measurements were carried out on ground with a double torsion pendulum that allowed us to estimate both the torque injected into the sensor when a control force is applied and, conversely, the force leaking into the translational degree of freedom due to the applied torque.The values measured on our apparatus (the engineering model of the LISA Pathfinder sensor) agree to within 0.2% (over a maximum measured crosstalk of 1%) with predictions of a mathematical model when measuring force to torque crosstalk, while it is somewhat larger than expected (up to 3.5%) when measuring torque to force crosstalk. However, the values in the relevant range, i.e. when the TM is well centered ( ± 10 μm) in the sensor, remain smaller than 0.2%, satisfying the LISA Pathfinder requirements.

Relating MBSE to Spacecraft Development: A NASA Pathfinder

NASA Technical Reports Server (NTRS)

Othon, Bill

2016-01-01

The NASA Engineering and Safety Center (NESC) has sponsored a Pathfinder Study to investigate how Model Based Systems Engineering (MBSE) and Model Based Engineering (MBE) techniques can be applied by NASA spacecraft development projects. The objectives of this Pathfinder Study included analyzing both the products of the modeling activity, as well as the process and tool chain through which the spacecraft design activities are executed. Several aspects of MBSE methodology and process were explored. Adoption and consistent use of the MBSE methodology within an existing development environment can be difficult. The Pathfinder Team evaluated the possibility that an "MBSE Template" could be developed as both a teaching tool as well as a baseline from which future NASA projects could leverage. Elements of this template include spacecraft system component libraries, data dictionaries and ontology specifications, as well as software services that do work on the models themselves. The Pathfinder Study also evaluated the tool chain aspects of development. Two chains were considered: 1. The Development tool chain, through which SysML model development was performed and controlled, and 2. The Analysis tool chain, through which both static and dynamic system analysis is performed. Of particular interest was the ability to exchange data between SysML and other engineering tools such as CAD and Dynamic Simulation tools. For this study, the team selected a Mars Lander vehicle as the element to be designed. The paper will discuss what system models were developed, how data was captured and exchanged, and what analyses were conducted.

NASA's Webb "Pathfinder Telescope" Successfully Completes First Super-Cold Optical Test

NASA Image and Video Library

2017-12-08

Testing is crucial part of NASA's success on Earth and in space. So, as the actual flight components of NASA's James Webb Space Telescope come together, engineers are testing the non-flight equipment to ensure that tests on the real Webb telescope later goes safely and according to plan. Recently, the "pathfinder telescope," or just “Pathfinder,” completed its first super-cold optical test that resulted in many first-of-a-kind demonstrations. "This test is the first dry-run of the equipment and procedures we will use to conduct an end-to-end optical test of the flight telescope and instruments," said Mark Clampin, Webb telescope Observatory Project Scientist at NASA's Goddard Space Flight Center in Greenbelt, Maryland. "It provides confidence that once the flight telescope is ready, we are fully prepared for a successful test of the flight hardware." The Pathfinder is a non-flight replica of the Webb telescope’s center section backplane, or “backbone,” that includes mirrors. The flight backplane comes in three segments, a center section and two wing-like parts, all of which will support large hexagonal mirrors on the Webb telescope. The pathfinder only consists of the center part of the backplane. However, during the test, it held two full size spare primary mirror segments and a full size spare secondary mirror to demonstrate the ability to optically test and align the telescope at the planned operating temperatures of -400 degrees Fahrenheit (-240 Celsius). Read more: www.nasa.gov/feature/goddard/nasas-webb-pathfinder-telesc... Credit: NASA/Goddard/Chris Gunn NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Coexpression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain.

PubMed

Huang, Chia-Yi; Chu, Dachen; Hwang, Wei-Chao; Tsaur, Meei-Ling

2012-11-01

Precise axon pathfinding is crucial for establishment of the initial neuronal network during development. Pioneer axons navigate without the help of preexisting axons and pave the way for follower axons that project later. Voltage-gated ion channels make up the intrinsic electrical activity of pioneer axons and regulate axon pathfinding. To elucidate which channel molecules are present in pioneer axons, immunohistochemical analysis was performed to examine 14 voltage-gated ion channels (Kv1.1-Kv1.3, Kv3.1-Kv3.4, Kv4.3, Cav1.2, Cav1.3, Cav2.2, Nav1.2, Nav1.6, and Nav1.9) in nine axonal tracts in the developing rat forebrain, including the optic nerve, corpus callosum, corticofugal fibers, thalamocortical axons, lateral olfactory tract, hippocamposeptal projection, anterior commissure, hippocampal commissure, and medial longitudinal fasciculus. We found A-type K⁺ channel Kv3.4 in both pioneer axons and early follower axons and L-type Ca²⁺ channel Cav1.2 in pioneer axons and early and late follower axons. Spatially, Kv3.4 and Cav1.2 were colocalized with markers of pioneer neurons and pioneer axons, such as deleted in colorectal cancer (DCC), in most fiber tracts examined. Temporally, Kv3.4 and Cav1.2 were expressed abundantly in most fiber tracts during axon pathfinding but were downregulated beginning in synaptogenesis. By contrast, delayed rectifier Kv channels (e.g., Kv1.1) and Nav channels (e.g., Nav1.2) were absent from these fiber tracts (except for the corpus callosum) during pathfinding of pioneer axons. These data suggest that Kv3.4 and Cav1.2, two high-voltage-activated ion channels, may act together to control Ca²⁺ -dependent electrical activity of pioneer axons and play important roles during axon pathfinding. Copyright © 2012 Wiley Periodicals, Inc.

Assessment of Mars Pathfinder landing site predictions

USGS Publications Warehouse

Golombek, M.P.; Moore, H.J.; Haldemann, A.F.C.; Parker, T.J.; Schofield, J.T.

1999-01-01

Remote sensing data at scales of kilometers and an Earth analog were used to accurately predict the characteristics of the Mars Pathfinder landing site at a scale of meters. The surface surrounding the Mars Pathfinder lander in Ares Vallis appears consistent with orbital interpretations, namely, that it would be a rocky plain composed of materials deposited by catastrophic floods. The surface and observed maximum clast size appears similar to predictions based on an analogous surface of the Ephrata Fan in the Channeled Scabland of Washington state. The elevation of the site measured by relatively small footprint delay-Doppler radar is within 100 m of that determined by two-way ranging and Doppler tracking of the spacecraft. The nearly equal elevations of the Mars Pathfinder and Viking Lander 1 sites allowed a prediction of the atmospheric conditions with altitude (pressure, temperature, and winds) that were well within the entry, descent, and landing design margins. High-resolution (~38 m/pixel) Viking Orbiter 1 images showed a sparsely cratered surface with small knobs with relatively low slopes, consistent with observations of these features from the lander. Measured rock abundance is within 10% of that expected from Viking orbiter thermal observations and models. The fractional area covered by large, potentially hazardous rocks observed is similar to that estimated from model rock distributions based on data from the Viking landing sites, Earth analog sites, and total rock abundance. The bulk and fine-component thermal inertias measured from orbit are similar to those calculated from the observed rock size-frequency distribution. A simple radar echo model based on the reflectivity of the soil (estimated from its bulk density), and the measured fraction of area covered by rocks was used to approximate the quasi-specular and diffuse components of the Earth-based radar echos. Color and albedo orbiter data were used to predict the relatively dust free or unweathered surface around the Pathfinder lander compared to the Viking landing sites. Comparisons with the experiences of selecting the Viking landing sites demonstrate the enormous benefit the Viking data and its analyses and models had on the successful predictions of the Pathfinder site. The Pathfinder experience demonstrates that, in certain locations, geologic processes observed in orbiter data can be used to infer surface characteristics where those processes dominate over other processes affecting the Martian surface layer. Copyright 1999 by the American Geophysical Union.

Intramuscular pressures in antigravity muscles using gravity-independent, pneumatic hardware.

PubMed

Macias, Brandon R; Minocha, Ranjeet; Cutuk, Adnan A; Hill, James; Shiau, Jonathon; Hargens, Alan R

2008-08-01

Resistive exercise helps prevent muscle atrophy in microgravity, but better exercise equipment is needed. Therefore, the purpose of this study was to determine if a pneumatic, gravity-independent leg-press device (LPD) provides sufficient force to leg musculature. We hypothesized that intramuscular pressure (IMP), a quantitative index of muscle force, is greater in the antigravity superficial posterior and deep posterior compartments than in the non-antigravity anterior compartment during bilateral leg-press exercise. Millar pressure transducers were inserted into the anterior, lateral, superficial posterior, and deep posterior muscle compartments of the left leg of eight healthy subjects (three women, five men). Subjects were supine on the Keiser SX-1, a pneumatic LPD. Then maximal voluntary contraction (MVC) was determined; each subject performed three consecutive voluntary contractions at approximately 18%, 50%, and 100% MVC while continuously measuring IMP. Repeated measures ANOVA were used to determine differences of IMPs between compartments and loads. The magnitudes of IMP (mean +/- SEM) at 18 - 3% (abbreviated approximately 18%), 50%, and 100% MVC in the superficial and deep posterior compartments were significantly greater than that in the anterior compartment during exercise (P < 0.05). Additionally, IMPs in all four compartments significantly rose as resistance increased at approximately 18%, 50%, and 100% MVC (P < 0.05). The LPD provides significantly increased resistance to all four compartments, but with greater loading of the antigravity compartments as compared to the non-antigravity compartment. Since antigravity muscles of the leg are contained primarily in the superficial and deep posterior compartments, the LPD may help prevent muscle atrophy associated with microgravity.

Improving AIDS Care After Trauma (ImpACT): Pilot Outcomes of a Coping intervention Among HIV-Infected Women with Sexual Trauma in South Africa.

PubMed

Sikkema, Kathleen J; Mulawa, Marta I; Robertson, Corne; Watt, Melissa H; Ciya, Nonceba; Stein, Dan J; Cherenack, Emily M; Choi, Karmel W; Kombora, Matapelo; Joska, John A

2018-03-01

Improving AIDS Care after Trauma (ImpACT), a coping intervention for HIV-infected women with sexual abuse histories, was evaluated for feasibility and potential efficacy in a public clinic in Cape Town, South Africa. Sixty-four participants were enrolled prior to starting antiretroviral therapy (ART). After completing baseline assessments, participants were randomly assigned to standard of care (SoC: three adherence counseling sessions) or ImpACT (SoC plus four individual and three group sessions). Participants completed assessments at 3 months (after individual sessions) and 6 months post-baseline. In exploratory analysis of primary outcomes, ImpACT participants, compared to SoC, reported greater reductions in avoidance and arousal symptoms of PTSD and greater increases in ART adherence motivation at 3 months. Clinically significant decreases in overall PTSD symptoms were also demonstrated at 3 months. These effects continued as trends at the 6-month assessment, in addition to increases in social/spiritual coping. In analysis of secondary outcomes, high levels of non-adherence to ART and poor care engagement were evident at 6 months, with no differences between study arms. A trauma-focused, culturally-adapted individual intervention delivered by a non-specialist in the HIV care setting is feasible and acceptable. Preliminary findings suggest ImpACT has potential to reduce PTSD symptoms and increase ART adherence motivation, but a more intensive intervention may be needed to improve and maintain care engagement among this population. ClinicalTrials.gov NCT02223390.

Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

PubMed Central

Yang, Zhengrong; Wang, Chi; Zhou, Qingxian; An, Jianli; Hildebrandt, Ellen; Aleksandrov, Luba A; Kappes, John C; DeLucas, Lawrence J; Riordan, John R; Urbatsch, Ina L; Hunt, John F; Brouillette, Christie G

2014-01-01

Detergent interaction with extramembranous soluble domains (ESDs) is not commonly considered an important determinant of integral membrane protein (IMP) behavior during purification and crystallization, even though ESDs contribute to the stability of many IMPs. Here we demonstrate that some generally nondenaturing detergents critically destabilize a model ESD, the first nucleotide-binding domain (NBD1) from the human cystic fibrosis transmembrane conductance regulator (CFTR), a model IMP. Notably, the detergents show equivalent trends in their influence on the stability of isolated NBD1 and full-length CFTR. We used differential scanning calorimetry (DSC) and circular dichroism (CD) spectroscopy to monitor changes in NBD1 stability and secondary structure, respectively, during titration with a series of detergents. Their effective harshness in these assays mirrors that widely accepted for their interaction with IMPs, i.e., anionic > zwitterionic > nonionic. It is noteworthy that including lipids or nonionic detergents is shown to mitigate detergent harshness, as will limiting contact time. We infer three thermodynamic mechanisms from the observed thermal destabilization by monomer or micelle: (i) binding to the unfolded state with no change in the native structure (all detergent classes); (ii) native state binding that alters thermodynamic properties and perhaps conformation (nonionic detergents); and (iii) detergent binding that directly leads to denaturation of the native state (anionic and zwitterionic). These results demonstrate that the accepted model for the harshness of detergents applies to their interaction with an ESD. It is concluded that destabilization of extramembranous soluble domains by specific detergents will influence the stability of some IMPs during purification. PMID:24652590

Response properties of the pharyngeal branch of the glossopharyngeal nerve for umami taste in mice and rats.

PubMed

Kitagawa, Junichi; Takahashi, Yoshihiro; Matsumoto, Shigeji; Shingai, Tomio

2007-04-24

Many studies have reported the mechanism underlying umami taste. However, there are no investigations of responses to umami stimuli taste originating from chemoreceptors in the pharyngeal region. The pharyngeal branch of the glossopharyngeal nerve (GPN-ph) innervating the pharynx has unique responses to taste stimulation that differs from responses of the chorda tympani nerve and lingual branch of the glossopharyngeal nerve. Water evokes robust response, but NaCl solutions at physiological concentrations do not elicit responses. The present study was designed to examine umami taste (chemosensory) responses in the GPN-ph. Response characteristics to umami taste were compared between mice and rats. In mice, stimulation with compounds eliciting umami taste (0.1M monosodium L-glutamate (MSG), 0.01M inosine monophosphate (IMP) and the mixture of 0.1M MSG+0.01M IMP) evoked higher responses than application of distilled water (DW). However, synergistic response of a mixture of 0.1M MSG+0.01M IMP was not observed. In rats, there is no significant difference between the responses to umami taste (0.1M MSG, 0.01M IMP and the mixture of 0.1M MSG+0.01M IMP) and DW. Monopotassium glutamate (MPG) was used in rats to examine the contribution of the sodium component of MSG on the response. Stimulation with 0.1M MPG evoked a higher response when compared with responses to DW. The present results suggest that umami taste compounds are effective stimuli of the chemoreceptors in the pharynx of both mice and rats.

Molecular detection of metallo-β-lactamase genes, bla IMP-1, bla VIM-2 and bla SPM-1 in imipenem resistant Pseudomonas aeruginosa isolated from clinical specimens in teaching hospitals of Ahvaz, Iran.

PubMed

Moosavian, Mojtaba; Rahimzadeh, Mohammad

2015-02-01

Carbapenem resistant Pseudomonas aeruginosa is a serious cause of nosocomial infections. The main purpose of the study is to determine the prevalence rate of imipenem resistant Pseudomonas aeruginosa carrying metallo-ß-lactamase (MBL) genes. 236 Pseudomonas aeruginosa isolates were collected from teaching hospitals of Ahvaz University of Medical Sciences during a period of 9 months in 2012. These strains were identified using conventional microbiological tests. The susceptibility of isolates to antibiotics were assessed using disk diffusion test. The IMP-EDTA combination disk phenotypic test was performed for detection of MBL producing strains. Finally, polymerase chain reaction (PCR) was performed to detect MBL genes, bla IMP-1, bla VIM-2 and bla SPM-1 in imipenem resistant strains. Out of 236 examined isolates, 122 isolates (51.4%) were resistant to imipenem. The IMP-EDTA combination test showed that among 122 imipenem resistant strains, 110 strains (90%) were phenotipically MBL producers. Additionally, the results of PCR method showed that 2 strains (1.6%) and 67strains (55%) of imipenem resistant Pseudomonas aeruginosa isolates contained bla VIM-2 and bla IMP-1 genes respectively. No SPM-1gene was found in the examined samples. Resistance of P. aeruginosa isolates to imipenem due to MBL enzymes is increasing in Ahavaz. Because of clinical significance of this kind of resistance, rapid detection of MBL producing strains and followed by appropriate treatment is necessary to prevent the spreading of these organisms.

Identification and characterization of metallo-β-lactamases producing Pseudomonas aeruginosa clinical isolates in University Hospital from Zanjan Province, Iran.

PubMed

Doosti, Masoumeh; Ramazani, Ali; Garshasbi, Maryam

2013-01-01

Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. A total of 70 P. aeruginosa isolates were identified from patients admitted at intensive care units. The antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method and for production of MBL using double-disk synergy test (DDST). After DNA extraction, the presence of blaVIM and blaIMP genes and class 1 integron were detected by PCR. Most of the isolates were resistant to meropenem, cefotaxime and imipenem (IPM). Also, 44/70 (62.85%) IPM resistant isolates were confirmed by DDST. Of the 44 clinical isolates, 41 (93%) isolates showed MIC≥4 µg/ml for IPM. Based on the DDST results, 36 (87.8%) were confirmed to be MBL producers. PCR amplification showed that 23/41 (56%) carried blaVIM and 10/41 (24.3%) possessed blaIMP gene. Also, 31/44 (70.5%) isolates contained class 1 integron gene. Our results highlight that the genes for Verona integron-encoded metallo-β-lactamase, IPM β-lactamases and class 1 integrons were predominantly present among the IPM-resistant P. aeruginosa tested in our province and also the frequency of blaVIM type is higher than blaIMP. This is the first report of P. aeruginosa strains producing blaIMP with high frequency from Zanjan province of Iran.

Taste sensitivity for monosodium glutamate and an increased liking of dietary protein.

PubMed

Luscombe-Marsh, Natalie D; Smeets, Astrid J P G; Westerterp-Plantenga, Margriet S

2008-04-01

The aim of the present study was to determine individuals' taste threshold for monosodium glutamate (MSG) alone and in combination with inosine 5'-monophosphate (IMP-5) and to examine if this threshold was related to an increase in sensory properties (including pleasantness of taste) and/or to one's preference for dietary protein over carbohydrate and fat. Using the triangle tasting method, the taste threshold was determined for thirty-six women and twenty-four men. Thresholds varied from zero to infinite as determined using a clear soup with added MSG in the concentration range of 0.1 to 0.8 % (w/w) MSG. Subjects rated fourteen sensory properties of the soup and also their 'liking', 'eating frequency' and 'preference' of twenty-two common high-protein, high-carbohydrate and high-fat food items. The taste threshold (and therefore sensitivity) of MSG was lowered from 0.33 (sem 0.24) to 0.26 (sem 0.22) % MSG when 0.25 % (w/w) IMP-5 was added. None of the sensory properties assessed was associated with the taste threshold of MSG +/- 0.25 % IMP-5 in the overall study population. However, the taste descriptor 'meatiness' was associated with the threshold data for individuals who could taste concentrations of

Effect of bolus volume and viscosity on pharyngeal automated impedance manometry variables derived for broad Dysphagia patients.

PubMed

Omari, Taher I; Dejaeger, Eddy; Tack, Jan; Van Beckevoort, Dirk; Rommel, Nathalie

2013-06-01

Automated impedance manometry (AIM) analysis measures swallow variables defining bolus timing, pressure, contractile vigour, and bolus presence, which are combined to derive a swallow risk index (SRI) correlating with aspiration. In a heterogeneous cohort of dysphagia patients, we assessed the impact of bolus volume and viscosity on AIM variables. We studied 40 patients (average age = 46 years). Swallowing of boluses was recorded with manometry, impedance, and videofluoroscopy. AIMplot software was used to derive functional variables: peak pressure (PeakP), pressure at nadir impedance (PNadImp), time from nadir impedance to peak pressure (TNadImp-PeakP), the interval of impedance drop in the distal pharynx (flow interval, FI), upper oesophageal sphincter (UES) relaxation interval (UES RI), nadir UES pressure (Nad UESP), UES intrabolus pressure (UES IBP), and UES resistance. The SRI was derived using the formula SRI = (FI * PNadImp)/(PeakP * (TNadImp-PeakP + 1)) * 100. A total of 173 liquid, 44 semisolid, and 33 solid boluses were analysed. The SRI was elevated in relation to aspiration. PeakP increased with volume. SRI was not significantly altered by bolus volume. PNadImp, UES IBP, and UES resistance increased with viscosity. SRI was lower with increased viscosity. In patients with dysphagia, the SRI is elevated in relation to aspiration, reduced by bolus viscosity, and not affected by bolus volume. These data provide evidence that pharyngeal AIM analysis may have clinical utility for assessing deglutitive aspiration risk to liquid boluses.

Hearing evaluation of intratympanic methylprednisolone perfusion for refractory sudden sensorineural hearing loss.

PubMed

She, Wandong; Dai, Yanhong; Du, Xiaoping; Yu, Chenjie; Chen, Feng; Wang, Junguo; Qin, Xiaoming

2010-02-01

To investigate the effectiveness and safety of intratympanic methylprednisolone perfusion (IMP) through a microcatheter in patients with sudden sensorineural hearing loss (SSNHL) who failed a conventional treatment. Prospective clinical study. This study was conducted in Nanjing Drum Tower Hospital, Nanjing University Medical School. Patients who had failed a minimum 10-day conventional treatment were included. Twenty-six patients in the study group (SG) received methylprednisolone perfusion through a microcatheter placed into the tympanum once a day for 10 days and the conventional treatment. Twenty-three patients who received a second conventional treatment (no steroid) served as the comparison group (CG). All patients were followed up for three months after the end of treatment. The effective rates for SG and CG were 50 percent (61.9% when only patients with an interval from onset to IMP < or = 60 days were included) and 21.7 percent, respectively (chi(2) = 4.194, P = 0.041). The pure-tone average improvement was 20.2 +/- 15.6 dB in SG, and 9.2 +/- 13.7 dB in CG (z = 2.51, P = 0.011). In SG, hearing improvement at low frequencies was better than that at high frequencies. The interval from onset to IMP affected the efficacy of IMP. IMP through a microcatheter is a promising treatment for refractory SSNHL. The data suggest that the treatment may be more effective when administered at the earlier stages of SSNHL when the conventional treatment has failed. Copyright 2010 American Academy of Otolaryngology-Head and Neck Surgery Foundation. Published by Mosby, Inc. All rights reserved.

LROC Targeted Observations for the Next Generation of Scientific Exploration

NASA Astrophysics Data System (ADS)

Jolliff, B. L.

2015-12-01

Imaging of the Moon at high spatial resolution (0.5 to 2 mpp) by the Lunar Reconnaissance Orbiter Camera (LROC) Narrow Angle Cameras (NAC) plus topographic data derived from LROC NAC and WAC (Wide Angle Camera) and LOLA (Lunar Orbiting Laser Altimeter), coupled with recently obtained hyperspectral NIR and thermal data, permit studies of composition, mineralogy, and geologic context at essentially an outcrop scale. Such studies pave the way for future landed and sample return missions for high science priority targets. Among such targets are (1) the youngest volcanic rocks on the Moon, including mare basalts formed as recently as ~1 Ga, and irregular mare patches (IMPs) that appear to be even younger [1]; (2) volcanic rocks and complexes with compositions more silica-rich than mare basalts [2-4]; (3) differentiated impact-melt deposits [5,6], ancient volcanics, and compositional anomalies within the South Pole-Aitken basin; (4) exposures of recently discovered key crustal rock types in uplifted structures such as essentially pure anorthosite [7] and spinel-rich rocks [8]; and (5) frozen volatile-element-rich deposits in polar areas [9]. Important data sets include feature sequences of paired NAC images obtained under similar illumination conditions, NAC geometric stereo, from which high-resolution DTMs can be made, and photometric sequences useful for assessing composition in areas of mature cover soils. Examples of each of these target types will be discussed in context of potential future missions. References: [1] Braden et al. (2014) Nat. Geo. 7, 787-791. [2] Glotch et al. (2010) Science, 329, 1510-1513. [3] Greenhagen et al. (2010) Science, 329, 1507-1509. [4] Jolliff et al. (2011) Nat. Geo. 4, 566-571. [5] Vaughan et al (2013) PSS 91, 101-106. [6] Hurwitz and Kring (2014) J. Geophys. Res. 119, 1110-1133 [7] Ohtake et al. (2009) Nature, 461, 236-241 [8] Pieters et al. (2014) Am. Min. 99, 1893-1910. [9] Colaprete et al. (2010) Science 330, 463-468.

Cerebral blood-flow tomography: xenon-133 compared with isopropyl-amphetamine-iodine-123: concise communication

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lassen, N.A.; Henriksen, L.; Holm, S.

1983-01-01

Tomographic maps of local cerebral blood flow (CBF) were obtained with xenon-133 and with isopropyl-amphetamine-iodine-123 (IMP) in 11 subjects: one normal, two tumor cases, and eight cerebrovascular cases. A highly sensitive four-face, rapidly rotating, single-photon emission tomograph was used. The Xe-133 flow maps are essentially based on the average Xe-133 concentration over the initial 2 min during and after an inhalation of the inert gas lasting 1 min. These maps agreed very well with the early IMP maps obtained over the initial 10 min following an i.v. bolus injection. The subsequent IMP tomograms showed a slight decrease in contrast amountingmore » to appr. five percentage points in the CBF ratio between diseased and contralateral areas. It is concluded that Xe-133 is more practical: low cost, available on a 7-day basis, easily repeatable, quantifiable without the need for arterial sampling, and with low radiation exposure to patient and personnel. On the other hand, IMP gives an image of slightly higher resolution. It also introduces a new class of iodinated brain-seeking compounds allowing, perhaps, imaging of other functions more important than mere blood flow.« less

Relationship of the eye uptake of N-isopropyl-p-(/sup 123/I)iodoamphetamine to melanin production

DOE Office of Scientific and Technical Information (OSTI.GOV)

Holman, B.L.; Wick, M.M.; Kaplan, M.L.

1984-03-01

Eye uptake has been a potential concern with N-isopropyl-p-(/sup 123/I)iodoamphetamine (I-123 IMP) because it has been observed in certain animal species. The authors have investigated the cause of the eye uptake and its relationship to melanin synthesis. In a 1-yr-old cynomolgus monkey, high concentration of the tracer was seen in the eyes regardless of the type of anesthesia (pentobarbital or ketamine) or the oral administration of Lugol's solution. The eye uptake at 24 hr after injection of I-123 IMP was equally high in an 8-yr-old rhesus monkey. The ratio of radioactivity in the eye of black compared with white albinomore » mice was 10:1 at 30 min, 18:1 at 2 hr and 36:1 at 24 hr after injection if I-123 IMP. No eye uptake above soft-tissue background was seen in five patients at 2, 24, and 48 hr after injection. I-123 IMP is avidly incorporated into melanocytes actively producing melanin, but substantially less in melanocytes where production of melanin has ceased as in the human eye.« less

Modification of mature non-reducible collagen cross-link concentrations in bovine m. gluteus medius and semitendinosus with steer age at slaughter, breed cross and growth promotants.

PubMed

Roy, B C; Sedgewick, G; Aalhus, J L; Basarab, J A; Bruce, H L

2015-12-01

Increased meat toughness with animal age has been attributed to mature trivalent collagen cross-link formation. Intramuscular trivalent collagen cross-link content may be decreased by reducing animal age at slaughter and/or inducing muscle re-modeling with growth promotants. This hypothesis was tested in m. gluteus medius (GM) and m. semitendinosus (ST) from 112 beef steers finished at either 12 to 13 (rapid growth) or 18 to 20 (slow growth) months of age. Hereford-Aberdeen Angus (HAA) or Charolais-Red Angus (CRA) steers were randomly assigned to receive implants (IMP), ractopamine (RAC), both IMP and RAC, or none (control). RAC decreased pyridinoline (mol/mol collagen) and IMP increased Ehrlich chromogen (EC) (mol/mol collagen) in the GM. In the ST, RAC increased EC (mol/mol collagen) but decreased EC (nmol/g raw muscle) in slow growing CRA steers. Also, IMP increased ST pyridinoline (nmol/g raw muscle) of slow-growing HAA steers. Results indicated alteration of perimysium collagen cross-links content in muscle in response to growth promotants. Copyright © 2015 Elsevier Ltd. All rights reserved.

Recent Development of IMP ECR Ion Sources

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhao, H.W.; Zhang, Z.M.; Sun, L.T.

2005-03-15

Great efforts have been made to develop highly charged ECR ion sources for application of heavy ion accelerator and atomic physics research at IMP in the past few years. The latest development of ECR ion sources at IMP is briefly reviewed. Intense beams with high and intermediate charge states have been produced from IMP LECR3 by optimization of the ion source conditions including rf frequency extended up to 18GHz. 1.1 emA of Ar8+ and 325 e{mu} A of Ar11+ were produced. Dependence of beam emittance on those key parameters of ECR ion source, beam extraction and space charge compensation weremore » experimentally studied at LECR3. Furthermore, an advanced superconducting ECR ion source named SECRAL is being constructed. SECRAL is designed to operate at rf frequency 18-28GHz with axial mirror magnetic fields 3.6-4.0 Tesla at injection, 2.2 Tesla at extraction and sextupole field 2.0 Tesla at the wall. The superconducting magnet with sextupole and three solenoids was tested in a test-cryostat and 95% of designed fields were reached. Construction status and planed schedule of SECRAL are presented.« less

Formation and photoluminescence of GaAs{sub 1−x}N{sub x} dilute nitride achieved by N-implantation and flash lamp annealing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gao, Kun, E-mail: k.gao@hzdr.de; Helm, M.; Technische Universität Dresden, 01062 Dresden

2014-07-07

In this paper, we present the fabrication of dilute nitride semiconductor GaAs{sub 1−x}N{sub x} by nitrogen-ion-implantation and flash lamp annealing (FLA). N was implanted into the GaAs wafers with atomic concentration of about x{sub imp1} = 0.38% and x{sub imp2} = 0.76%. The GaAs{sub 1−x}N{sub x} layer is regrown on GaAs during FLA treatment in a solid phase epitaxy process. Room temperature near band-edge photoluminescence (PL) has been observed from the FLA treated GaAs{sub 1−x}N{sub x} samples. According to the redshift of the near band-edge PL peak, up to 80% and 44% of the implanted N atoms have been incorporated into the lattice bymore » FLA for x{sub imp1} = 0.38% and x{sub imp2} = 0.76%, respectively. Our investigation shows that ion implantation followed by ultrashort flash lamp treatment, which allows for large scale production, exhibits a promising prospect on bandgap engineering of GaAs based semiconductors.« less

A long non-coding RNA, LncMyoD, regulates skeletal muscle differentiation by blocking IMP2-mediated mRNA translation.

PubMed

Gong, Chenguang; Li, Zhizhong; Ramanujan, Krishnan; Clay, Ieuan; Zhang, Yunyu; Lemire-Brachat, Sophie; Glass, David J

2015-07-27

Increasing evidence suggests that long non-coding RNAs (LncRNAs) represent a new class of regulators of stem cells. However, the roles of LncRNAs in stem cell maintenance and myogenesis remain largely unexamined. For this study, hundreds of intergenic LncRNAs were identified that are expressed in myoblasts and regulated during differentiation. One of these LncRNAs, termed LncMyoD, is encoded next to the Myod gene and is directly activated by MyoD during myoblast differentiation. Knockdown of LncMyoD strongly inhibits terminal muscle differentiation, largely due to a failure to exit the cell cycle. LncMyoD directly binds to IGF2-mRNA-binding protein 2 (IMP2) and negatively regulates IMP2-mediated translation of proliferation genes such as N-Ras and c-Myc. While the RNA sequence of LncMyoD is not well conserved between human and mouse, its locus, gene structure, and function are preserved. The MyoD-LncMyoD-IMP2 pathway elucidates a mechanism as to how MyoD blocks proliferation to create a permissive state for differentiation. Copyright © 2015 Elsevier Inc. All rights reserved.

Airbag Retraction

NASA Image and Video Library

1997-07-05

This image shows that the Mars Pathfinder airbags have been successfully retracted, allowing safe deployment of the rover ramps. The Sojourner rover, still in its deployed position, is at center image, and rocks are visible in the background. Mars Pathfinder landed successfully on the surface of Mars today at 10:07 a.m. PDT. http://photojournal.jpl.nasa.gov/catalog/PIA00617

Pathfinder: The Geospatial Intelligence Magazine Serving the Front Line, March/April 2009. Volume 7, Number 2

DTIC Science & Technology

2009-04-01

ADDRESS(ES) National Geospatial-Intelligence Agency,4600 Sangamore Rd Mail Stop D-54,Bethesda,MD, 20816 -5003 8. PERFORMING ORGANIZATION REPORT NUMBER 9...Bethesda, MD 20816 -5003 Telephone: (301) 227-7388, DSN 287-7388 E-mail: pathfinder@nga.mil Director Vice Adm. Robert B. Murrett, U.S. Navy Deputy

Global comparisons between the modified Pathfinder derived sea surface temperature and skin temperatures from the along-track scanning radiometer on board ERS-2: how close are we getting?

NASA Technical Reports Server (NTRS)

Vazquez, J.

2001-01-01

Sea Surface Temperatures (SST) as derived from the Pathfinder Sea Surface Temperature Data Set and the Along-Track Scanning Radiometer on-board the European Remote Sensing Satellite provide a unique opportunity for comparing two independent SST data sets.

SLS Pathfinder Segments Car Train Departure

NASA Image and Video Library

2016-03-02

An Iowa Northern locomotive, contracted by Goodloe Transportation of Chicago, departs from NASA’s Kennedy Space Center in Florida, with two containers on railcars for transport to the Jay Jay railroad yard. The containers held two pathfinders, or test versions, of solid rocket booster segments for NASA’s Space Launch System rocket that were delivered to the Rotation, Processing and Surge Facility (RPSF). Inside the RPSF, the Ground Systems Development and Operations Program and Jacobs Engineering, on the Test and Operations Support Contract, will conduct a series of lifts, moves and stacking operations using the booster segments, which are inert, to prepare for Exploration Mission-1, deep-space missions and the journey to Mars. The pathfinder booster segments are from Orbital ATK in Utah.

James Webb Space Telescope Optical Telescope Element/Integrated Science Instrument Module (OTIS) Status

NASA Technical Reports Server (NTRS)

Feinberg, Lee; Voyton, Mark; Lander, Juli; Keski-Kuha, Ritva; Matthews, Gary

2016-01-01

The James Webb Space Telescope Optical Telescope Element (OTE) and Integrated Science Instrument Module (ISIM) are integrated together to form the OTIS. Once integrated, the OTIS undergoes primary mirror center of curvature optical tests, electrical and operational tests, acoustics and vibration testing at the Goddard Space Flight Center before being shipped to the Johnson Space Center for cryogenic optical testing of the OTIS. In preparation for the cryogenic optical testing, the JWST project has built a Pathfinder telescope and has completed two Optical Ground System Equipment (OGSE) cryogenic optical tests with the Pathfinder. In this paper, we will summarize optical test results to date and status the final Pathfinder test and the OTIS integration and environmental test preparations

LISA Pathfinder Spacecraft Artist Concept

NASA Image and Video Library

2015-12-03

This artist's concept shows ESA's LISA Pathfinder spacecraft, which launched on Dec. 3, 2015, from Kourou, French Guiana, will help pave the way for a mission to detect gravitational waves. LISA Pathfinder, led by the European Space Agency (ESA), is designed to test technologies that could one day detect gravitational waves. Gravitational waves, predicted by Einstein's theory of general relativity, are ripples in spacetime produced by any accelerating body. But the waves are so weak that Earth- or space-based observatories would likely only be able to directly detect such signals coming from massive astronomical systems, such as binary black holes or exploding stars. Detecting gravitational waves would be an important piece in the puzzle of how our universe began. http://photojournal.jpl.nasa.gov/catalog/PIA20196

SLS Pathfinder Segments Car Train Departure

NASA Image and Video Library

2016-03-02

An Iowa Northern locomotive, contracted by Goodloe Transportation of Chicago, departs from the Rotation, Processing and Surge Facility (RPSF) at NASA’s Kennedy Space Center in Florida, with two containers on railcars for transport to the NASA Jay Jay railroad yard. The containers held two pathfinders, or test versions, of solid rocket booster segments for NASA’s Space Launch System rocket that were delivered to the RPSF. Inside the RPSF, the Ground Systems Development and Operations Program and Jacobs Engineering, on the Test and Operations Support Contract, will conduct a series of lifts, moves and stacking operations using the booster segments, which are inert, to prepare for Exploration Mission-1, deep-space missions and the journey to Mars. The pathfinder booster segments are from Orbital ATK in Utah.

Visible Wavelength Spectroscopy of Ferric Minerals: A Key Tool for Identification of Ancient Martian Aqueous Environments

NASA Technical Reports Server (NTRS)

Murchie, Scott L.; Bell, J. F., III; Morris, Richard V.

2000-01-01

The mineralogic signatures of past aqueous alteration of a basaltic Martian crust may include iron oxides and oxyhydroxides, zeolites, carbonates, phyllosilicates, and silica. The identities, relative abundances, and crystallinities of the phases formed in a particular environment depend on physicochemical conditions. At one extreme, hot spring environments may be characterized by smectite-chlorite to talc-kaolinite silicate assemblages, plus crystalline ferric oxides dominated by hematite. However, most environments, including cold springs, pedogenic layers, and ponded surface water, are expected to deposit iron oxides and oxyhydroxides, carbonates, and smectite-dominated phyllosilicates. A substantial fraction of the ferric iron is expected to occur in nanophase form, with the exact mineralogy strongly influenced by Eh-pH conditions. Detection of these phases has been an objective of a large body of terrestrial telescopic, Mars orbital, and landed spectral investigations and in situ compositional measurements. However, clear identifications of many of these phases is lacking. Neither carbonate nor silica has been unequivocally detected by any method. Although phyllosilicates may occur near the limit of detection by remote sensing, in general they appear to occur in only poorly crystalline form. In contrast, compelling evidence for ferric iron minerals has been gathered by recent telescopic investigations, the Imager for Mars Pathfinder (IMP), and the Thermal Emission Spectrometer (TES) on the Mars Global Surveyor (MGS). These data yield two crucial findings: (1) In the global, high spatial resolution TES data set, highly crystalline ferric iron (as coarse-grained 'gray' hematite) has been recognized but with only very limited spatial occurrence and (2) Low-resolution telescopic reflectance spectroscopy, very limited orbital reflectance spectroscopy, and landed multispectral imaging provide strong indications that at least two broad classes of ferric iron minerals are commonplace in non-dust covered regions.

Structure of Cryptosporidium IMP dehydrogenase bound to an inhibitor with in vivo antiparasitic activity

DOE PAGES

Kim, Youngchang; Makowska-Grzyska, Magdalena; Gorla, Suresh Kumar; ...

2015-04-21

Inosine 5´-monophosphate dehydrogenase (IMPDH) is a promising target for the treatment of Cryptosporidium infections. Here, the structure of C. parvum IMPDH ( CpIMPDH) in complex with inosine 5´-monophosphate (IMP) and P131, an inhibitor with in vivo anticryptosporidial activity, is reported. P131 contains two aromatic groups, one of which interacts with the hypoxanthine ring of IMP, while the second interacts with the aromatic ring of a tyrosine in the adjacent subunit. In addition, the amine and NO 2 moieties bind in hydrated cavities, forming water-mediated hydrogen bonds to the protein. The design of compounds to replace these water molecules is amore » new strategy for the further optimization of C. parvum inhibitors for both antiparasitic and antibacterial applications.« less

Differential diagnosis of bilateral parietal abnormalities in I-123 IMP SPECT imaging

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kuwabara, Y.; Ichiya, Y.; Otsuka, M.

1990-12-01

This report discusses the clinical significance of bilateral parietal abnormalities on I-123 IMP SPECT imaging in 158 patients with cerebral disorders. This pattern was seen in 15 out of 21 patients with Alzheimer's disease; it was also seen in 4 out of 5 patients with Parkinson's disease with dementia, in 3 out of 17 patients with vascular dementia, in 1 out of 36 patients with cerebral infarction without dementia, in 1 out of 2 patients with hypoglycemia, and in 1 out of 2 patients with CO intoxication. Detection of bilateral parietal abnormalities is a useful finding in the diagnosis ofmore » Alzheimer's disease, but one should keep in mind that other cerebral disorders may also show a similar pattern with I-123 IMP SPECT imaging.« less

IMS/Satellite Situation Center report: Predicted orbit plots for IMP-J-1976

NASA Technical Reports Server (NTRS)

1975-01-01

Predicted orbit plots for the IMP-J satellite were given for the time period January-December 1976. These plots are shown in three projections. The time period covered by each set of projections is 12 days and 6 hours, corresponding approximately to the period of IMP-J. The three coordinate systems used are the Geocentric Solar Ecliptic system (GSE), the Geocentric Solar Magnetospheric system (GSM), and the Solar Magnetic system (SM). For each of the three projections, time ticks and codes are given on the satellite trajectories. The codes are interpreted in the table at the base of each plot. Time is given in the table as year/day/decimal hour, and the total time covered by each plot is shown at the bottom of each table.

Structure of Cryptosporidium IMP dehydrogenase bound to an inhibitor with in vivo antiparasitic activity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Youngchang; Makowska-Grzyska, Magdalena; Gorla, Suresh Kumar

2015-04-21

Inosine 5'-monophosphate dehydrogenase (IMPDH) is a promising target for the treatment ofCryptosporidiuminfections. Here, the structure ofC. parvumIMPDH (CpIMPDH) in complex with inosine 5'-monophosphate (IMP) and P131, an inhibitor within vivoanticryptosporidial activity, is reported. P131 contains two aromatic groups, one of which interacts with the hypoxanthine ring of IMP, while the second interacts with the aromatic ring of a tyrosine in the adjacent subunit. In addition, the amine and NO 2moieties bind in hydrated cavities, forming water-mediated hydrogen bonds to the protein. The design of compounds to replace these water molecules is a new strategy for the further optimization ofC. parvuminhibitorsmore » for both antiparasitic and antibacterial applications.« less

IRAS asteroid families

NASA Technical Reports Server (NTRS)

Veeder, G. J.; Williams, J. G.; Tedesco, E. F.; Matson, D. L.

1991-01-01

The Infrared Astronomical Satellite (IRAS) sampled the entire asteroid population at wavelengths from 12 to 100 microns during its 1983 all sky survey. The IRAS Minor Planet Survey (IMPS) includes updated results for more recently numbered as well as other additional asteroids with reliable orbital elements. Albedos and diameters were derived from the observed thermal emission and assumed absolute visual magnitudes and then entered into the IMPS database at the Infrared Processing and Analysis Center (IPAC) for members of the Themis, Eos, Koronis and Maria asteroid families and compared with their visual colors. The IMPS results for the small (down to about 20 km) asteroids within these major families confirm trends previously noted for their larger members. Each of these dynamical families which are defined by their similar proper elements appears to have homogeneous physical properties.

Planetary Image Geometry Library

NASA Technical Reports Server (NTRS)

Deen, Robert C.; Pariser, Oleg

2010-01-01

The Planetary Image Geometry (PIG) library is a multi-mission library used for projecting images (EDRs, or Experiment Data Records) and managing their geometry for in-situ missions. A collection of models describes cameras and their articulation, allowing application programs such as mosaickers, terrain generators, and pointing correction tools to be written in a multi-mission manner, without any knowledge of parameters specific to the supported missions. Camera model objects allow transformation of image coordinates to and from view vectors in XYZ space. Pointing models, specific to each mission, describe how to orient the camera models based on telemetry or other information. Surface models describe the surface in general terms. Coordinate system objects manage the various coordinate systems involved in most missions. File objects manage access to metadata (labels, including telemetry information) in the input EDRs and RDRs (Reduced Data Records). Label models manage metadata information in output files. Site objects keep track of different locations where the spacecraft might be at a given time. Radiometry models allow correction of radiometry for an image. Mission objects contain basic mission parameters. Pointing adjustment ("nav") files allow pointing to be corrected. The object-oriented structure (C++) makes it easy to subclass just the pieces of the library that are truly mission-specific. Typically, this involves just the pointing model and coordinate systems, and parts of the file model. Once the library was developed (initially for Mars Polar Lander, MPL), adding new missions ranged from two days to a few months, resulting in significant cost savings as compared to rewriting all the application programs for each mission. Currently supported missions include Mars Pathfinder (MPF), MPL, Mars Exploration Rover (MER), Phoenix, and Mars Science Lab (MSL). Applications based on this library create the majority of operational image RDRs for those missions. A Java wrapper around the library allows parts of it to be used from Java code (via a native JNI interface). Future conversions of all or part of the library to Java are contemplated.

International Space Station Data Collection for Disaster Response

NASA Technical Reports Server (NTRS)

Stefanov, William L.; Evans, Cynthia A.

2015-01-01

Remotely sensed data acquired by orbital sensor systems has emerged as a vital tool to identify the extent of damage resulting from a natural disaster, as well as providing near-real time mapping support to response efforts on the ground and humanitarian aid efforts. The International Space Station (ISS) is a unique terrestrial remote sensing platform for acquiring disaster response imagery. Unlike automated remote-sensing platforms it has a human crew; is equipped with both internal and externally-mounted remote sensing instruments; and has an inclined, low-Earth orbit that provides variable views and lighting (day and night) over 95 percent of the inhabited surface of the Earth. As such, it provides a useful complement to autonomous sensor systems in higher altitude polar orbits. NASA remote sensing assets on the station began collecting International Disaster Charter (IDC) response data in May 2012. The initial NASA ISS sensor systems responding to IDC activations included the ISS Agricultural Camera (ISSAC), mounted in the Window Observational Research Facility (WORF); the Crew Earth Observations (CEO) Facility, where the crew collects imagery using off-the-shelf handheld digital cameras; and the Hyperspectral Imager for the Coastal Ocean (HICO), a visible to near-infrared system mounted externally on the Japan Experiment Module Exposed Facility. The ISSAC completed its primary mission in January 2013. It was replaced by the very high resolution ISS SERVIR Environmental Research and Visualization System (ISERV) Pathfinder, a visible-wavelength digital camera, telescope, and pointing system. Since the start of IDC response in 2012 there have been 108 IDC activations; NASA sensor systems have collected data for thirty-two of these events. Of the successful data collections, eight involved two or more ISS sensor systems responding to the same event. Data has also been collected by International Partners in response to natural disasters, most notably JAXA and Roscosmos/Energia through the Urugan program.

Evaluation of the fit of zirconia copings fabricated by direct and indirect digital impression procedures.

PubMed

Lee, Bora; Oh, Kyung Chul; Haam, Daewon; Lee, Joon-Hee; Moon, Hong-Seok

2018-02-07

Intraoral scanners are effective for direct digital impression when dental restorations are fabricated using computer-aided design and computer-aided manufacturing (CAD-CAM); however, if the abutment tooth cannot be dried completely or the prepared margin is placed subgingivally, accurate digital images cannot always be guaranteed. The purpose of this in vitro study was to compare the internal and marginal discrepancies of zirconia copings fabricated directly using an intraoral scanner with those fabricated indirectly with impression scanning. Forty-five resin dies fabricated with a 3-dimensional (3D) printer were divided into 3 groups: direct scanning (DS), impression scanning (IMP), and lost-wax casting (LW). For the DS group, a resin die was scanned with an intraoral scanner (Trios; 3Shape), whereas for the IMP group, impressions made with polyether were scanned with a cast scanner (D700; 3Shape). The zirconia copings were fabricated in the same way in the DS and IMP groups. For the LW group, impressions were made in the same way as in the IMP group, and Ni-Cr alloy copings were fabricated using LW. The marginal and internal discrepancies of the copings were measured by cementing them onto resin dies, embedding them in acrylic resin, and sectioning them in a buccolingual direction. The cement layer was measured, and the Kruskal-Wallis test was used to detect significant differences (α=.05). A nonparametric Friedman test was also performed to compare the measurements of each group by location (α=.05). The mean marginal discrepancies in the DS, IMP, and LW groups were 18.1 ±9.8, 23.2 ±17.2, and 32.3 ±18.6 μm (mean ±standard deviation), respectively. The mean internal discrepancies of the DS, IMP, and LW groups in the axial area were 38.0 ±9.1, 47.0 ±16.3, and 36.5 ±15.8 μm, and those in the occlusal area were 36.7 ±16.9, 33.4 ±21.6, and 44.5 ±31.9 μm, respectively. No statistically significant differences were found in marginal or internal discrepancies among groups (P>.05). Within the limitations of this study, the zirconia copings fabricated with CAD-CAM using different digitization methods and Ni-Cr copings fabricated using the lost-wax technique and casting produced clinically acceptable marginal and internal discrepancies. No significant differences were found among the DS, IMP, and LW groups. Copyright © 2017 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.

Montmorillonite, Oligonucleotides, RNA and Origin of Life

NASA Astrophysics Data System (ADS)

Ertem, Gözen

2004-12-01

Na-montmorillonite prepared from Volclay by the titration method facilitates the self-condensation of ImpA, the 5'-phosphorimidazolide derivative of adenosine. As was shown by AE-HPLC analysis and selective enzymatic hydrolysis of products, oligo(A)s formed in this reaction are 10 monomer units long and contain 67% 3',5'-phosphodiester bonds (Ferris and Ertem, 1992a). Under the same reaction conditions, 5'-phosphorimidazolide derivatives of cytidine, uridine and guanosine also undergo self-condensation producing oligomers containing up to 12-14 monomer units for oligo(C)s to 6 monomer units for oligo(G)s. In oligo(C)s and oligo(U)s, 75-80% of the monomers are linked by 2',5'-phosphodiester bonds. Hexamer and higher oligomers isolated from synthetic oligo(C)s formed by montmorillonite catalysis, which contain both 3',5'- and 2',5'-linkages, serve as catalysts for the non-enzymatic template directed synthesis of oligo(G)s from activated monomer 2-MeImpG, guanosine 5'-phospho-2-methylimidazolide (Ertem and Ferris, 1996). Pentamer and higher oligomers containing exclusively 2',5'-linkages, which were isolated from the synthetic oligo(C)s, also serve as templates and produce oligo(G)s with both 2',5'- and 3',5'-phosphodiester bonds. Kinetic studies on montmorillonite catalyzed elongation rates of oligomers using the computer program SIMFIT demonstrated that the rate constants for the formation of oligo(A)s increased in the order of 2-mer <3-mer <4-mer ... <7-mer (Kawamura and Ferris, 1994). A decameric primer, dA(pdA)8pA bound to montmorillonite was elongated to contain up to 50 monomer units by daily addition of activated monomer ImpA to the reaction mixture (Ferris, Hill and Orgel, 1996). Analysis of dimer fractions formed in the montmorillonite catalyzed reaction of binary and quaternary mixtures of ImpA, ImpC, 2-MeImpG and ImpU suggested that only a limited number of oligomers could have formed on the primitive Earth rather than equal amounts of all possible isomers (Ertem and Ferris, 2000). Formation of phosphodiester bonds between mononucleotides by montmorillonite catalysis is a fascinating discovery, and a significant step forward in efforts to find out how the first RNA-like oligomers might have formed in the course of chemical evolution. However, as has been pointed out in several publications, these systems should be regarded as models rather than a literal representation of prebiotic chemistry (Orgel, 1998; Joyce and Orgel, 1999; Schwartz, 1999).