The Use of Meteorlogical Data to Improve Contrail Detection in Thermal Imagery over Ireland.

NASA Technical Reports Server (NTRS)

Whelan, Gillian M.; Cawkwell, Fiona; Mannstein, Hermann; Minnis, Patrick

2009-01-01

Aircraft induced contrails have been found to have a net warming influence on the climate system, with strong regional dependence. Persistent linear contrails are detectable in 1 Km thermal imagery and, using an automated Contrail Detection Algorithm (CDA), can be identified on the basis of their different properties at the 11 and 12 m w av.el enTgthshe algorithm s ability to distinguish contrails from other linear features depends on the sensitivity of its tuning parameters. In order to keep the number of false identifications low, the algorithm imposes strict limits on contrail size, linearity and intensity. This paper investigates whether including additional information (i.e. meteorological data) within the CDA may allow for these criteria to be less rigorous, thus increasing the contrail-detection rate, without increasing the false alarm rate.

K-12 Students as Ground Observers of Contrails in Support of Scientific Research

NASA Technical Reports Server (NTRS)

Chambers, Lin H.; Moore, Susan W.; Fischer, Joyce D.; Sepulveda, Roberto; Clark, C.

2004-01-01

Scientists are very interested in the formation of contrails, both the type and the coverage. To be detected by a satellite-born instrument, the contrail must be of a certain size, which means that some contrails go undetected. The K-12 education community is assisting with the study of contrails by participating in a network of student observers. To provide a venue for student contrail observations, the GLOBE Contrails protocol was developed as part of the GLOBE Atmospheric Science protocols. The first year of observations has provided a rich resource for researcher.

Measurements of upper troposheric humidity at low temperatures during CRYSTAL-FACE

NASA Technical Reports Server (NTRS)

Herman, Robert L.; Heymsfield, Andrew J.; Ridley, Brian A.; Bui, Paul T.

2003-01-01

Aircraft condensation trails (contrails) and thin cirrus were studied by instruments on the NASA WB-57F high-altitude aircraft during contrails and optically thing cirrus are contrasted by different levels of supersaturation with respect to ice. During the July 13, 2002, flight of the WB-57F aircraft intercepted visible contrails produced by both the WB-57F and ER-2 aircraft. These contrails were located immediately below the local tropopause, where ambient temperatures were very low (-76 degree C). The contrails were clearly indicated by an abrupt increase in NO and a simultaneous, abrupt decrease in ice supersaturation. With in the contrail, the relative humidity was close to 130% with respect to ice, higher than expected from theory. Outside the contrails was a persistent layer of subvisible currus extending from approximately 13 to 15 km altitude. This layer was characterized y significant supersaturations because the ambient concentrations of ice particles were insufficient to significantly deplete the ice supersaturation. We will discuss in situ measurements and models simulations of humidity.

Exploring the Radiative Effect and Climate Impact of Contaminated Contrails

NASA Astrophysics Data System (ADS)

Yi, B.; Yang, P.; Minnis, P.; Duda, D. P.

2015-12-01

As an impact of human aviation activities, contrails have drawn a great deal of attention. There have been numerous investigations into the contrail properties, radiative effects, and climate impact. However, very little effort has been focused on the impact of contaminated contrails. Generated by the combustion process within the aircraft engine, the aerosols and exhaust gases frequently influence contrail formation. Contrail ice crystals contaminated by soot particles have been found to exhibit dramatically different light scattering properties from those of pristine crystals. In this study, we employ state-of-the-art light scattering computational capabilities to calculate the single-scattering properties of soot-contaminated contrails. The contaminated contrail particle is assumed to be a hexagonal ice column containing several soot particles. The invariant imbedding T-matrix method and the Ray-by-Ray geometry optics method are combined to construct a simplified yet novel set of contaminated contrail optical properties. The bulk optical properties are calculated based on the data set and are parameterized for use in the Community Atmospheric Model. Using global contrail retrievals from satellite remote sensing observations in 2006 and 2012, simulations are conducted using the general circulation model to analyze contaminated contrail radiative effects as well as their climatic sensitivities. Our results show that the contaminated contrail is significantly more absorbing than pristine contrail in the shortwave spectrum. As a result, much stronger contrail radiative impact and climate feedback are found. Several sensitivity studies are also implemented to quantify the effect of contrail contamination.

Changes in Cirrus Cloudiness and their Relationship to Contrails

NASA Technical Reports Server (NTRS)

Minnis, Patrick; Ayers, J. Kirk; Palikonda, Rabindra; Doelling, David R.; Schumann, Ulrich; Gierens, Klaus

2001-01-01

Condensation trails, or contrails, formed in the wake of high-altitude aircraft have long been suspected of causing the formation of additional cirrus cloud cover. More cirrus is possible because 10 - 20% of the atmosphere at typical commercial flight altitudes is clear but ice-saturated. Since they can affect the radiation budget like natural cirrus clouds of equivalent optical depth and microphysical properties, contrail -generated cirrus clouds are another potential source of anthropogenic influence on climate. Initial estimates of contrail radiative forcing (CRF) were based on linear contrail coverage and optical depths derived from a limited number of satellite observations. Assuming that such estimates are accurate, they can be considered as the minimum possible CRF because contrails often develop into cirrus clouds unrecognizable as contrails. These anthropogenic cirrus are not likely to be identified as contrails from satellites and would, therefore, not contribute to estimates of contrail coverage. The mean lifetime and coverage of spreading contrails relative to linear contrails are needed to fully assess the climatic effect of contrails, but are difficult to measure directly. However, the maximum possible impact can be estimated using the relative trends in cirrus coverage over regions with and without air traffic. In this paper, the upper bound of CRF is derived by first computing the change in cirrus coverage over areas with heavy air traffic relative to that over the remainder of the globe assuming that the difference between the two trends is due solely to contrails. This difference is normalized to the corresponding linear contrail coverage for the same regions to obtain an average spreading factor. The maximum contrail-cirrus coverage, estimated as the product of the spreading factor and the linear contrail coverage, is then used in the radiative model to estimate the maximum potential CRF for current air traffic.

Impact of biofuels on contrail warming

NASA Astrophysics Data System (ADS)

Caiazzo, Fabio; Agarwal, Akshat; Speth, Raymond L.; Barrett, Steven R. H.

2017-11-01

Contrails and contrail-cirrus may be the largest source of radiative forcing (RF) attributable to aviation. Biomass-derived alternative jet fuels are a potentially major way to mitigate the climate impacts of aviation by reducing lifecycle CO2 emissions. Given the up to 90% reduction in soot emissions from paraffinic biofuels, the potential for a significant impact on contrail RF due to the reduction in contrail-forming ice nuclei (IN) remains an open question. We simulate contrail formation and evolution to quantify RF over the United States under different emissions scenarios. Replacing conventional jet fuels with paraffinic biofuels generates two competing effects. First, the higher water emissions index results in an increase in contrail occurrence (~ +8%). On the other hand, these contrails are composed of larger diameter crystals (~ +58%) at lower number concentrations (~ -75%), reducing both contrail optical depth (~ -29%) and albedo (~ -32%). The net changes in contrail RF induced by switching to biofuels range from -4% to +18% among a range of assumed ice crystal habits (shapes). In comparison, cleaner burning engines (with no increase in water emissions index) result in changes to net contrail RF ranging between -13% and +5% depending on habit. Thus, we find that even 67% to 75% reductions in aircraft soot emissions are insufficient to substantially reduce warming from contrails, and that the use of biofuels may either increase or decrease contrail warming—contrary to previous expectations of a significant decrease in warming.

Contrail formation in the tropopause region caused by emissions from an Ariane 5 rocket

NASA Astrophysics Data System (ADS)

Voigt, Ch.; Schumann, U.; Graf, K.

2016-07-01

Rockets directly inject water vapor and aerosol into the atmosphere, which promotes the formation of ice clouds in ice supersaturated layers of the atmosphere. Enhanced mesospheric cloud occurrence has frequently been detected near 80-kilometer altitude a few days after rocket launches. Here, unique evidence for cirrus formation in the tropopause region caused by ice nucleation in the exhaust plume from an Ariane 5-ECA rocket is presented. Meteorological reanalysis data from the European Centre for Medium-Range Weather Forecasts show significant ice supersaturation at the 100-hectopascal level in the American tropical tropopause region on November 26, 2011. Near 17-kilometer altitudes, the temperatures are below the Schmidt-Appleman threshold temperature for rocket condensation trail formation on that day. Immediately after the launch from the Ariane 5-ECA at 18:39 UT (universal time) from Kourou, French Guiana, the formation of a rocket contrail is detected in the high resolution visible channel from the SEVIRI (Spinning Enhanced Visible and InfraRed Imager) on the METEOSAT9 satellite. The rocket contrail is transported to the south and its dispersion is followed in SEVIRI data for almost 2 h. The ice crystals predominantly nucleated on aluminum oxide particles emitted by the Ariane 5-ECA solid booster and further grow by uptake of water vapor emitted from the cryogenic main stage and entrained from the ice supersaturated ambient atmosphere. After rocket launches, the formation of rocket contrails can be a frequent phenomenon under ice supersaturated conditions. However, at present launch rates, the global climate impact from rocket contrail cirrus in the tropopause region is small.

Three-dimensional large-eddy simulations of the early phase of contrail-to-cirrus transition: effects of atmospheric turbulence and radiative transfer

DOE PAGES

Paoli, Roberto; Thouron, Odile; Cariolle, Daniel; ...

2017-12-08

Here, this article presents the results from numerical experiments of the early phase of contrail-cirrus formation using a limited set of fully three-dimensional, high-resolution large-eddy-simulations. The focus is laid on the interplay between atmospheric turbulence and the radiative transfer (and to a limited extent the ambient ice relative humidity), and how this interaction affects the contrail evolution and the characteristics of the resulting contrail-cirrus one hour after emission. Turbulence is sustained via a large-scale stochastic forcing that creates a non-uniform shear in addition to pure turbulent fluctuations. This effect manifests in the formation of vertically sheared structures of ice crystals.more » When radiative transfer is activated, ice tends to redistribute more uniformly along the vertical direction forming spotty vertical structures. For the conditions analyzed in this study, atmospheric turbulence, inclusive of non-uniform turbulent shear and turbulent fluctuations, affects primarily the contrail width whereas the microphysical properties such ice water path and ice mass are controlled by radiative transfer and relative humidity.« less

Three-dimensional large-eddy simulations of the early phase of contrail-to-cirrus transition: effects of atmospheric turbulence and radiative transfer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paoli, Roberto; Thouron, Odile; Cariolle, Daniel

Here, this article presents the results from numerical experiments of the early phase of contrail-cirrus formation using a limited set of fully three-dimensional, high-resolution large-eddy-simulations. The focus is laid on the interplay between atmospheric turbulence and the radiative transfer (and to a limited extent the ambient ice relative humidity), and how this interaction affects the contrail evolution and the characteristics of the resulting contrail-cirrus one hour after emission. Turbulence is sustained via a large-scale stochastic forcing that creates a non-uniform shear in addition to pure turbulent fluctuations. This effect manifests in the formation of vertically sheared structures of ice crystals.more » When radiative transfer is activated, ice tends to redistribute more uniformly along the vertical direction forming spotty vertical structures. For the conditions analyzed in this study, atmospheric turbulence, inclusive of non-uniform turbulent shear and turbulent fluctuations, affects primarily the contrail width whereas the microphysical properties such ice water path and ice mass are controlled by radiative transfer and relative humidity.« less

Synoptic Control of Contrail Cirrus Life Cycles and Their Modification Due to Reduced Soot Number Emissions

NASA Astrophysics Data System (ADS)

Bier, A.; Burkhardt, U.; Bock, L.

2017-11-01

The atmospheric state, aircraft emissions, and engine properties determine formation and initial properties of contrails. The synoptic situation controls microphysical and dynamical processes and causes a wide variability of contrail cirrus life cycles. A reduction of soot particle number emissions, resulting, for example, from the use of alternative fuels, strongly impacts initial ice crystal numbers and microphysical process rates of contrail cirrus. We use the European Centre/Hamburg (ECHAM) climate model version 5 including a contrail cirrus modul, studying process rates, properties, and life cycles of contrail cirrus clusters within different synoptic situations. The impact of reduced soot number emissions is approximated by a reduction in the initial ice crystal number, exemplarily studied for 80%. Contrail cirrus microphysical and macrophysical properties can depend much more strongly on the synoptic situation than on the initial ice crystal number. They can attain a large cover, optical depth, and ice water content in long-lived and large-scale ice-supersaturated areas, making them particularly climate-relevant. In those synoptic situations, the accumulated ice crystal loss due to sedimentation is increased by around 15% and the volume of contrail cirrus, exceeding an optical depth of 0.02, and their short-wave radiative impact are strongly decreased due to reduced soot emissions. These reductions are of little consequence in short-lived and small-scale ice-supersaturated areas, where contrail cirrus stay optically very thin and attain a low cover. The synoptic situations in which long-lived and climate-relevant contrail cirrus clusters can be found over the eastern U.S. occur in around 25% of cases.

Properties of individual contrails: a compilation of observations and some comparisons

NASA Astrophysics Data System (ADS)

Schumann, Ulrich; Baumann, Robert; Baumgardner, Darrel; Bedka, Sarah T.; Duda, David P.; Freudenthaler, Volker; Gayet, Jean-Francois; Heymsfield, Andrew J.; Minnis, Patrick; Quante, Markus; Raschke, Ehrhard; Schlager, Hans; Vázquez-Navarro, Margarita; Voigt, Christiane; Wang, Zhien

2017-01-01

Mean properties of individual contrails are characterized for a wide range of jet aircraft as a function of age during their life cycle from seconds to 11.5 h (7.4-18.7 km altitude, -88 to -31 °C ambient temperature), based on a compilation of about 230 previous in situ and remote sensing measurements. The airborne, satellite, and ground-based observations encompass exhaust contrails from jet aircraft from 1972 onwards, as well as a few older data for propeller aircraft. The contrails are characterized by mean ice particle sizes and concentrations, extinction, ice water content, optical depth, geometrical depth, and contrail width. Integral contrail properties include the cross-section area and total number of ice particles, total ice water content, and total extinction (area integral of extinction) per contrail length. When known, the contrail-causing aircraft and ambient conditions are characterized. The individual datasets are briefly described, including a few new analyses performed for this study, and compiled together to form a contrail library (COLI). The data are compared with results of the Contrail Cirrus Prediction (CoCiP) model. The observations confirm that the number of ice particles in contrails is controlled by the engine exhaust and the formation process in the jet phase, with some particle losses in the wake vortex phase, followed later by weak decreases with time. Contrail cross sections grow more quickly than expected from exhaust dilution. The cross-section-integrated extinction follows an algebraic approximation. The ratio of volume to effective mean radius decreases with time. The ice water content increases with increasing temperature, similar to non-contrail cirrus, while the equivalent relative humidity over ice saturation of the contrail ice mass increases at lower temperatures in the data. Several contrails were observed in warm air above the Schmidt-Appleman threshold temperature. The emission index of ice particles, i.e., the number of ice particles formed in the young contrail per burnt fuel mass, is estimated from the measured concentrations for estimated dilution; maximum values exceed 1015 kg-1. The dependence of the data on the observation methods is discussed. We find no obvious indication for significant contributions from spurious particles resulting from shattering of ice crystals on the microphysical probes.

Remote Sensing of Aircraft Contrails Using a Field Portable Digital Array Scanned Interferometer

NASA Technical Reports Server (NTRS)

Smith, William Hayden

1997-01-01

With a Digital Array Scanned Interferometer (DASI), we have obtained proof-of-concept observations with which we demonstrate DASI capabilities for the determination of contrail properties. These include the measurement of the cloud and soot microphysical parameters, as well, the abundances of specific pollutant species such as SO(sub x) or NO(sub x). From high quality hyperspectral data and using radiative transfer methods and atmospheric chemistry analysis in the data reduction and interpretation, powerful inferences concerning cloud formation, evolution and dissipation can be made. Under this sub-topic, we will integrate DASI with computer controlled scanning of the field-of-view to direct the sensor towards contrails and exhaust plumes for tracking the emitting vehicles. The optimum DASI wavelength sensitivity range for sensing contrails is 0.35 - 2.5 micron. DASI deploys on the ground or from aircraft to observe contrails in the vicinity. This enables rapid, accurate measurement of the temporal, spatial, and chemical evolution of contrails (or other plumes or exhaust sources) with a low cost, efficient sensor.

Sulfur Oxidation and Contrail Precursor Chemistry

NASA Technical Reports Server (NTRS)

DeWitt, Kenneth J.

2003-01-01

Sulfuric acid (H2SO4), formed in commercial aircraft operations via fuel-S (goes to) SO2 (goes to) SO3 (goes to) H2SO4 plays an important role in the formation of contrails. It is believed that the first step occurs inside the combustor, the second step in the engine exit nozzle, and the third step in the exhaust plume. Thus, measurements of the sulfur oxidation rates are critical to the understanding of contrail formation. Field measurements of contrails formed behind commercial aircraft indicate that significantly greater conversion of fuel-bound sulfur to sulfate aerosol occurs than can be explained by our current knowledge of contrail physics and chemistry. The conversion of sulfur from S(IV) to S(VI) oxidation state, required for sulfate aerosol formation, is thermodynamically favored for the conditions that exist within jet engines but is kinetically disfavored. The principal reaction pathway is O+SO2+M (goes to) SO3+M. The rates of this reaction have never been measured in the temperature and pressure regimes available to aircraft operation. In the first year (FY02) of this project, we performed a series of experiments to elucidate the rate information for the O+SO2+M (goes to) SO3+M reaction. The work performed is described following the proposed work plan. Because we used the H2/O2 system for an O-atom source and rate coefficients were obtained via computer simulation, construction of a reaction mechanism and either recalculation or estimation of thermodynamic properties of H(x)SO(y) species are described first.

Towards a Model Climatology of Relative Humidity in the Upper Troposphere for Estimation of Contrail and Contrail-Induced Cirrus

NASA Technical Reports Server (NTRS)

Selkirk, Henry B.; Manyin, M.; Ott, L.; Oman, L.; Benson, C.; Pawson, S.; Douglass, A. R.; Stolarski, R. S.

2011-01-01

The formation of contrails and contrail cirrus is very sensitive to the relative humidity of the upper troposphere. To reduce uncertainty in an estimate of the radiative impact of aviation-induced cirrus, a model must therefore be able to reproduce the observed background moisture fields with reasonable and quantifiable fidelity. Here we present an upper tropospheric moisture climatology from a 26-year ensemble of simulations using the GEOS CCM. We compare this free-running model's moisture fields to those obtained from the MLS and AIRS satellite instruments, our most comprehensive observational databases for upper tropospheric water vapor. Published comparisons have shown a substantial wet bias in GEOS-5 assimilated fields with respect to MLS water vapor and ice water content. This tendency is clear as well in the GEOS CCM simulations. The GEOS-5 moist physics in the GEOS CCM uses a saturation adjustment that prevents supersaturation, which is unrealistic when compared to in situ moisture observations from MOZAIC aircraft and balloon sondes as we will show. Further, the large-scale satellite datasets also consistently underestimate super-saturation when compared to the in-situ observations. We place these results in the context of estimates of contrail and contrail cirrus frequency.

Contrail Formation in Aircraft Wakes Using Large-Eddy Simulations

NASA Technical Reports Server (NTRS)

Paoli, R.; Helie, J.; Poinsot, T. J.; Ghosal, S.

2002-01-01

In this work we analyze the issue of the formation of condensation trails ("contrails") in the near-field of an aircraft wake. The basic configuration consists in an exhaust engine jet interacting with a wing-tip training vortex. The procedure adopted relies on a mixed Eulerian/Lagrangian two-phase flow approach; a simple micro-physics model for ice growth has been used to couple ice and vapor phases. Large eddy simulations have carried out at a realistic flight Reynolds number to evaluate the effects of turbulent mixing and wake vortex dynamics on ice-growth characteristics and vapor thermodynamic properties.

Calculations of condensation and chemistry in an aircraft contrail

NASA Technical Reports Server (NTRS)

Miake-Lye, Richard C.; Brown, R. C.; Anderson, M. R.; Kolb, C. E.

1994-01-01

The flow field, chemistry, and condensation nucleation behind a transport airplane are calculated in two regimes using two separate reacting flow codes: first the axisymmetric plume, then the three dimensional vortex wake. The included chemical kinetics equations follow the evolution of the NO(y) and SO(x) chemical families. In the plume regime, the chemistry is coupled with the binary homogeneous formation of sulfate condensation nuclei, where the calculated nucleation rates predict that copious quantities of H2SO4/H2O nuclei are produced in subnanometer sizes. These sulfate aerosols could play a major role in the subsequent condensation of water vapor and the formation of contrails under favorable atmospheric conditions.

Contrails and contrail-cirrus microphysics

DOT National Transportation Integrated Search

2008-01-25

Theme 4 of the ACCRI, Contrails and Contrail-Specific Microphysics, reviews the current : state of understanding of the science of contrails: 1) how they are formed, 2) their microphysical properties as they evolve, 3) how they develop into con...

Contrails and their impact on shortwave radiation and photovoltaic power production - a regional model study

NASA Astrophysics Data System (ADS)

Gruber, Simon; Unterstrasser, Simon; Bechtold, Jan; Vogel, Heike; Jung, Martin; Pak, Henry; Vogel, Bernhard

2018-05-01

A high-resolution regional-scale numerical model was extended by a parameterization that allows for both the generation and the life cycle of contrails and contrail cirrus to be calculated. The life cycle of contrails and contrail cirrus is described by a two-moment cloud microphysical scheme that was extended by a separate contrail ice class for a better representation of the high concentration of small ice crystals that occur in contrails. The basic input data set contains the spatially and temporally highly resolved flight trajectories over Central Europe derived from real-time data. The parameterization provides aircraft-dependent source terms for contrail ice mass and number. A case study was performed to investigate the influence of contrails and contrail cirrus on the shortwave radiative fluxes at the earth's surface. Accounting for contrails produced by aircraft enabled the model to simulate high clouds that were otherwise missing on this day. The effect of these extra clouds was to reduce the incoming shortwave radiation at the surface as well as the production of photovoltaic power by up to 10 %.

Spatial large-eddy simulations of contrail formation in the wake of an airliner

NASA Astrophysics Data System (ADS)

Paoli, R.

2015-12-01

Contrails and contrail-cirrus are the most uncertain contributors to aviation radiative forcing. In order to reduce this uncertainty one needs to gain more knowledge on the physicochemical processes occurring in the aircraft plume, which eventually lead to the transformation of contrails into cirrus. To that end, the accurate prediction of the number of activated particles and their spatial and size distributions at the end of the jet regime may be helpful to initialize simulations in the following vortex regime. We present the results from spatial large-eddy simulations (LES) of contrail formation in the near-field wake of a generic (but full-scale) airliner that is representative of those used in long-haul flights in current fleets. The flow around the aircraft has been computed using a RANS code taking into account the full geometry that include the engines and the aerodynamic set-up for cruise conditions. The data have been reconstructed at a plane closely behind the trailing edge of the wing and used as inflow boundary conditions for the LES. We employ fully compressible 3D LES coupled to Lagrangian microphysical module that tracks parcels of ice particles individually. The ice microphysical model is simple yet it contains the basic thermodynamic ingredients to model soot activation and water vapor deposition. Compared to one-dimensional models or even RANS, LES allow for more accurate predictions of the mixing between exhaust and ambient air. Hence, the number of activated particles and the ice growth rate can be also determined with higher accuracy. This is particularly crucial for particles located at the edge of the jet that experience large gradients of temperature and humidity. The results of the fully coupled LES (where the gas phase and the particles are solved together) are compared to offline simulations where the ice microphysics model is run using thermodynamic data from pre-calculated particle trajectories extracted from inert LES (where ice microphysics has been switched off).

The lifecycle and climate-impact of contrail cirrus

NASA Astrophysics Data System (ADS)

Schumann, Ulrich

2016-04-01

The lifecycle of contrail cirrus has to be understood as a prerequisite to compute its weather and climate impact for given airtraffic and meteorology. As a new concept, this study distinguishes between: 1) Externally limited contrail cirrus, where contrails form in moderately ice-supersaturated air, but ice particles stay small and contrails end by sublimation because of drying of the ambient air, e.g., when the ambient air subsides; 2) Internally limited contrail cirrus, where contrails form at high humidity with strong supersaturation or form in rising air masses, so that the ice particles grow until their fall speed gets large, and the ice particles finally fall to lower levels (e.g. in fall streaks). For both kinds of contrail cirrus, scaling laws are set up which show how the "Surface Forcing" (SF), i.e. the time-integral of optical depth times width (integral of ice particle number per flight distance times ice particle cross-section area times extinction efficiency) depends on the lifetime, on the number of ice particles per unit length, ambient humidity, uplift velocity, wind shear, turbulent mixing, and temperature. SF can be converted into an energy forcing (EF), from which the global radiative forcing can be evaluated, for given radiative Earth-atmosphere properties and traffic density. The scaling laws are tested by comparison to global contrail simulations with the most recent version of CoCiP (as in Schumann, 2012; and some changes), using ECMWF data and a global traffic data bases (ACCRI). The model assumes that contrail ice particles form initially mainly on soot, that the ice particles consume the ice supersaturation in the contrail plume, that the ice particle number decreases slightly with lifetime, and that interactions of contrails with ambient cirrus are weak. The scaling laws and the model allow estimating the climate impact of contrails as a function of a given aircraft and weather parameters. The results are compared to available results from airborne observation campaigns, like CONCERT and MLCIRRUS, from remote sensing, from large eddy simulations and global model studies. For externally limited contrails, the climate impact of contrails increases with about the square of the externally controlled lifetime and the third root of the number contrail ice particles per flight distance. For internally limited contrails, SF grows about linearly with this number.

Contrail Tracking and ARM Data Product Development

NASA Technical Reports Server (NTRS)

Duda, David P.; Russell, James, III

2005-01-01

A contrail tracking system was developed to help in the assessment of the effect of commercial jet contrails on the Earth's radiative budget. The tracking system was built by combining meteorological data from the Rapid Update Cycle (RUC) numerical weather prediction model with commercial air traffic flight track data and satellite imagery. A statistical contrail-forecasting model was created a combination of surface-based contrail observations and numerical weather analyses and forecasts. This model allows predictions of widespread contrail occurrences for contrail research on either a real-time basis or for long-term time scales. Satellite-derived cirrus cloud properties in polluted and unpolluted regions were compared to determine the impact of air traffic on cirrus.

Estimates of cloud radiative forcing in contrail clusters using GOES imagery

NASA Astrophysics Data System (ADS)

Duda, David P.; Minnis, Patrick; Nguyen, Louis

2001-03-01

Using data from the Geostationary Operational Environmental Satellite (GOES), the evolution of solar and longwave radiative forcing in contrail clusters is presented in several case studies. The first study examines contrails developing over the midwestern United States in a region of upper tropospheric moisture enhanced by the remnants of Hurricane Nora on September 26, 1997. Two other cases involve contrail clusters that formed over the Chesapeake Bay and the Atlantic Ocean on February 11 and March 5, 1999, respectively. The last study includes contrails forming over the tropical Pacific near Hawaii. Observations of tropical contrails near Hawaii show that the contrail optical properties are similar to those measured from satellite in the midlatitudes, with visible optical depths between 0.3 and 0.5 and particle sizes between 30 and 60 μm as the contrails mature into diffuse cloudiness. Radiative transfer model simulations of the tropical contrail case suggest that ice crystal shape may have an important effect on radiative forcing in contrails. The magnitudes of the observed solar and longwave radiative forcings were 5.6 and 3.2 W m-2 less than those from the corresponding model simulations, and these differences are attributed to the subpixel scale low clouds and uncertainties in the anisotropic reflectance and limb-darkening models used to estimate the observed forcing. Since the broadband radiative forcing in contrails often changes rapidly, contrail forcing estimates based only on the polar orbiting advanced very high resolution radiometer (AVHRR) data could be inaccurate due to the lack of sufficient temporal sampling.

Climate impact of contrails and contrail cirrus

DOT National Transportation Integrated Search

2008-01-25

Generally, the climatic impact of air traffic (of which a substantial part may be due to contrails and contrail cirrus) today (year 2000) amounts to 2-8% of the global radiative forcing associated with climate change. Due to the projected increase in...

Estimating the Contrail Impact on Climate Using the UK Met Office Model

NASA Astrophysics Data System (ADS)

Rap, A.; Forster, P. M.

2008-12-01

With air travel predicted to increase over the coming century, the emissions associated with air traffic are expected to have a significant warming effect on climate. According to current best estimates, an important contribution comes from contrails. However, as reported by the IPCC fourth assessment report, these current best estimates still have a high uncertainty. The development and validation of contrail parameterizations in global climate models is therefore very important. This current study develops a contrail parameterization within the UK Met Office Climate Model. Using this new parameterization, we estimate that for the 2002 traffic, the global mean annual contrail coverage is approximately 0.11%, a value which in good agreement with several other estimates. The corresponding contrail radiative forcing (RF) is calculated to be approximately 4 and 6 mWm-2 in all-sky and clear-sky conditions, respectively. These values lie within the lower end of the RF range reported by the latest IPCC assessment. The relatively high cloud masking effect on contrails observed by our parameterization compared with other studies is investigated, and a possible cause for this difference is suggested. The effect of the diurnal variations of air traffic on both contrail coverage and contrail RF is also investigated. The new parameterization is also employed in thirty-year slab-ocean model runs in order to give one of the first insights into contrail effects on daily temperature range and the climate impact of contrails.

Automatic Jet Contrail Detection and Segmentation

NASA Technical Reports Server (NTRS)

Weiss, J.; Christopher, S. A.; Welch, R. M.

1997-01-01

Jet contrails are an important subset of cirrus clouds in the atmosphere, and thin cirrus are thought to enhance the greenhouse effect due to their semi-transparent nature. They are nearly transparent to the solar energy reaching the surface, but they reduce the planetary emission to space due to their cold ambient temperatures. Having 'seeded' the environment, contrails often elongate and widen into cirrus-like features. However, there is great uncertainty regarding the impact of contrails on surface temperature and precipitation. With increasing numbers of subsonic aircraft operating in the upper troposphere, there is the possibility of increasing cloudiness which could lead to changes in the radiation balance. Automatic detection and seg- mentation of jet contrails in satellite imagery is important because (1) it is impractical to compile a contrail climatology by hand, and (2) with the segmented images it will be possible to retrieve contrail physical properties such as optical thickness, effective ice crystal diameter and emissivity.

Aircraft-type dependency of contrail evolution

NASA Astrophysics Data System (ADS)

Unterstrasser, S.; Görsch, N.

2014-12-01

The impact of aircraft type on contrail evolution is assessed using a large eddy simulation model with Lagrangian ice microphysics. Six different aircraft ranging from the small regional airliner Bombardier CRJ to the largest aircraft Airbus A380 are taken into account. Differences in wake vortex properties and fuel flow lead to considerable variations in the early contrail geometric depth and ice crystal number. Larger aircraft produce contrails with more ice crystals (assuming that the number of initially generated ice crystals per kilogram fuel is constant). These initial differences are reduced in the first minutes, as the ice crystal loss during the vortex phase is stronger for larger aircraft. In supersaturated air, contrails of large aircraft are much deeper after 5 min than those of small aircraft. A parameterization for the final vertical displacement of the wake vortex system is provided, depending only on the initial vortex circulation and stratification. Cloud resolving simulations are used to examine whether the aircraft-induced initial differences have a long-lasting mark. These simulations suggest that the synoptic scenario controls the contrail cirrus evolution qualitatively. However, quantitative differences between the contrail cirrus properties of the various aircraft remain over the total simulation period of 6 h. The total extinctions of A380-produced contrails are about 1.5 to 2.5 times higher than those from contrails of a Bombardier CRJ.

Radiative Forcing by Contrails

NASA Technical Reports Server (NTRS)

Meerkoetter, R.; Schumann, U.; Doelling, D. R.; Nakajima, T.; Tsushima, Y.

1999-01-01

A parametric study of the instantaneous radiative impact of contrails is presented using three different radiative transfer models for a series of model atmospheres and cloud parameters. Contrails are treated as geometrically and optically thin plane parallel homogeneous cirrus layers in a static atmospheres The ice water content is varied as a function of ambient temperature. The model atmospheres include tropical, mid-latitude, and subarctic summer and winter atmospheres Optically thin contrails cause a positive net forcing at top of the atmosphere. At the surface the radiative forcing is negative during daytime. The forcing increases with the optical depth and the amount of contrail cover. At the top of the atmosphere a mean contrail cover of 0.1% with average optical depth of 0.2 to 0.5 causes about 0.01 to 0.03 W/m(exp 2)a daily mean instantaneous radiative forcing. Contrails cool the surface during the day and heat the surface during the night, and hence reduce the daily temperature amplitude The net effect depends strongly on the daily variation of contrail cloud cover. The indirect radiative forcing due to particle changes in natural cirrus clouds may be of the same magnitude as the direct one due to additional cover.

3D-CFD Investigation of Contrails and Volatile Aerosols Produced in the Near-Field of an Aircraft Wake

NASA Astrophysics Data System (ADS)

Garnier, F.; Ghedhaifi, W.; Vancassel, X.; Khou, J. C.; Montreuil, E.

2015-12-01

Civil aviation contributes to degradation of air quality around airport (SOx, NOx, speciated hydrocarbons,…) and climate change through its emissions of greenhouse gases (CO2, water vapor), as well as particulate matters. These particles include soot particles formed in the combustor, volatile aerosols and contrails generated in the aircraft wake. Although the aircraft emissions represent today only about 3% of all those produced on the surface of the earth by other anthropogenic sources, they are mostly released in the very sensitive region of the upper troposphere/lower stratosphere. These emissions have a radiative effect reinforced by specific physical and chemical processes at high altitudes, such as cloud formation and ozone production. In this context, most of the work to-date assessed that the actual effect of aviation on the climate are affected by very large uncertainties, partly due to lack of knowledge on the mechanisms of new particles formation and growth processes in the exhaust plume of the aircraft. The engine exhaust gases are mixed in the ambient air under the influence of the interaction between the jet engine and the wing tip vortices. The characteristics of vortices as well as their interaction with the jet depend on the aircraft airframe especially on the wing geometry and the engine position (distance from the wing tip). The aim of this study is to examine the influence of aircraft parameters on contrail formation using a 3D CFD calculation based on a RANS (Reynolds Average Navier-Stokes) approach. Numerical simulations have been performed using CEDRE, the multiphysics ONERA code for energetics. CEDRE is a CFD code using finite volume methods and unstructured meshes. These meshes are especially appropriate when complex geometries are used. A transport model has been used for condensation of water vapor onto ice particles. Growth is evaluated using a modified Fick's law to mass transfer on particles. In this study, different aircraft configurations are analysed, a two-engine and a four-engine aircraft. The results show the influence on the engine location on the contrail formation in terms of size and distribution of ice particles in the near-field of the aircraft wake. Comparisons with reported observations in situ show a good agreement.

The effect of ice crystal shape on aircraft contrails

NASA Astrophysics Data System (ADS)

Meza Castillo, Omar E.

Aircraft contrails are a common phenomenon observed in the sky. They are formed mainly of water, from the ambient atmosphere and as a by-product of the combustion process, in the form of ice crystals. They have been identified as a potential contributor to global warming. Some contrails can be long-lived and create man-made cloud cover, thus possibly altering the radiative balance of the earth. There has been a great deal of research on various aspects of contrail development, but to date, little has been done on the influence of ice crystal shapes on the contrail evolution. In-situ studies have reported that young contrails are mainly quasi-spherical crystals while older contrails can have a much more diverse spectrum of possible shapes. The most common shapes found in contrails are quasi-spherical, hexagonal columns, hexagonal plates, and bullet rosettes. Numerical simulations of contrails to date typically have assumed "spherical" as the default ice shape. This work simulated contrail development with a large eddy simulation (LES) model that implemented both spherical and non-spherical shapes to examine the effects. The included shape effect parameters, such as capacitance coefficient, ventilation factor, Kelvin effect, fall velocity and ice crystal surface area, help to establish the shape difference in the results. This study also investigated initial sensitivities to an additional ice parameter, the ice deposition coefficient. The literature shows conflicting values for this coefficient over a wide range. In the course of this investigation a comparison of various ice metrics was made for simulations with different assumed crystal shapes (spheres, hexagonal columns, hexagonal plates, bullet rosettes and combination of shapes). The simulations were performed at early and late contrail time, with a range of ice crystal sizes, and with/without coupled radiation. In young and older contrails and without coupled radiation, the difference from the shape effect in ice crystal number, N(t), is not significant compared with the level of uncertainty. In young contrails, the difference between spherical and non-spherical shapes in N(t) is less than 7% for relatively large ice particles and 23% for relatively small ice particles. The ice mass, M(t), is not significantly affected by the crystal shapes, with less than 8% difference. However, the ice surface area, S(t), is the ice metric more sensitive to crystal shape, with a maximum difference of 68%. It increases at late time, though it is mainly governed by geometrical rather than dynamical effects. The small sensitivity to shape effects in the ice contrail metrics when radiation is not included suggests that the spherical shape will provide a reasonable representation for all shapes found in the in-situ studies. The radiation is included at late time, when the lasting effects of contrails are more critical. The inclusion of coupled radiation increases the level of dispersion in the results and hence increases slightly the differences due to shape effects. The small difference is also observed in the infrared heating rates of contrails.

Aircraft-Induced Hole Punch and Canal Clouds

NASA Astrophysics Data System (ADS)

Heymsfield, A. J.; Kennedy, P.; Massie, S. T.; Schmitt, C. G.; Wang, Z.; Haimov, S.; Rangno, A.

2009-12-01

The production of holes and channels in altocumulus clouds by two commercial turboprop aircraft is documented for the first time. An unprecedented data set combining in situ measurements from microphysical probes with remote sensing measurements from cloud radar and lidar, all operating from the NSF/NCAR C130 aircraft, as well as ground-based NOAA and CSU radars, is used to describe the radar/lidar properties of a hole punch cloud and channel and the ensuing ice microphysical properties and structure of the ice column that subsequently developed. Ice particle production by commercial turboprop aircraft climbing through clouds much warmer than the regions where contrails are produced has the potential to modify significantly the cloud microphysical properties and effectively seed them under some conditions. Jet aircraft may also be producing hole punch clouds when flying through altocumulus with supercooled droplets at heights lower than their normal cruise altitudes where contrails can form. Commercial aircraft therefore can generate ice and affect the clouds at temperatures as much as 30°C warmer than the -40°C contrail formation threshold temperature.

The engineering options for mitigating the climate impacts of aviation.

PubMed

Williams, Victoria

2007-12-15

Aviation is a growing contributor to climate change, with unique impacts due to the altitude of emissions. If existing traffic growth rates continue, radical engineering solutions will be required to prevent aviation becoming one of the dominant contributors to climate change. This paper reviews the engineering options for mitigating the climate impacts of aviation using aircraft and airspace technologies. These options include not only improvements in fuel efficiency, which would reduce carbon dioxide (CO2) emissions, but also measures to reduce non-CO2 impacts including the formation of persistent contrails. Integrated solutions to optimize environmental performance will require changes to airframes, engines, avionics, air traffic control systems and airspace design. While market-based measures, such as offset schemes and emissions trading, receive growing attention, this paper sets out the crucial role of engineering in the challenge to develop a 'green air traffic system'.

Case Study Analyses of the SUCCESS DC-8 Scanning Lidar Database

NASA Technical Reports Server (NTRS)

Uthe, Edward E.

2000-01-01

Under project SUCCESS (Subsonic Aircraft Contrail and Cloud Effects Special Study) funded by the Atmospheric Effects of Aviation Program, SRI International (SRI) developed an angular scanning backscatter lidar for operation on the NASA DC-8 research aircraft and deployed the scanning lidar during the SUCCESS field campaign. The primary purpose of the lidar was to generate real-time video displays of clouds and contrails above, ahead of, and below the DC-8 as a means to help position the aircraft for optimum cloud and contrail sampling by onboard in situ sensors, and to help extend the geometrical domain of the in situ sampling records. A large, relatively complex lidar database was collected and several data examples were processed to illustrate the value of the lidar data for interpreting the other data records collected during SUCCESS. These data examples were used to develop a journal publication for the special SUCCESS Geophysical Research Letters issue. The data examples justified data analyses of a larger part of the DC-8 lidar database and is the objective of the current study. Efficient processing of the SUCCESS DC-8 scanning lidar database required substantial effort to enhance hardware and software components of the data system that was used for the initial analyses. MATLAB instructions are used to generate altitude and distance color-coded lidar displays corrected for effects introduced by aircraft pitch and forward movement during an angular scan time interval. Onboard in situ sensor atmospheric measurements are propagated to distances ahead of the DC-8 using recorded aircraft velocity so that they can be plotted on the lidar displays for comparison with lidar remotely observed aerosol distributions. Resulting lidar and in situ sensor polar scan displays over extended sampling intervals are integrated into a time series movie format for 36 case studies. Contrails and clouds were detected to ranges of 15 km by the forward-viewing angular scanning lidar and were progressively mapped as the aircraft approached and penetrated them. Near aircraft lidar observations were much better correlated with in situ sensor observations than lidar observations at greater distances ahead of the aircraft. The major cause of this difference was thought to be the about 2 deg. offset of the lidar viewing direction from the flight direction. Contrail spatial distributions were not of the quality obtainable from ground-based lidar observations. This results because contrails tend to become horizontally stratified, vertical distance between angular lidar observations increases with increased distance from the aircraft, and erratic aircraft motions during an angular scan. The most useful lidar observations were made with lidar viewing directions of vertically upward or vertically downward. These provided real-time information on aircraft altitudes to achieve optimum in situ cloud and contrail sampling. At sampling altitudes, the forward viewing angular scanning observations were useful for fine-tuning the aircraft altitude for cloud and contrail penetration. Best information on cloud and contrail properties were obtained from vertically directed lidar observations as the aircraft performed a series of upward and downward penetrations of contrails. This operational mode was especially well suited for lidar and radiometric evaluation of cloud and contrail optical and radiative properties. The vertical viewing lidar detected ice crystals thought to be precipitating from an aircraft contrail and their scavenging by a cirrus cloud layer. The lidar display indicates that the crystals are effective for increasing cirrus cloud density. Vertical angular scanning observations can evaluate the sharp decrease in lidar backscatter for small off-vertical viewing directions that result from horizontally aligned ice crystals and perhaps can provide additional information on crystal shapes. The about 2 deg. offset of the lidar viewing direction from the flight direction is thought to have greatly degraded the forward-viewing angular scanning observations and this mode of operation was not fully evaluated. However, the reasoning for this capability remains valid and the angular scan presentations collected during this program justifies modification of the lidar pod for true forward direction lidar viewing during future cloud and contrail studies.

Automated detection of jet contrails using the AVHRR split window

NASA Technical Reports Server (NTRS)

Engelstad, M.; Sengupta, S. K.; Lee, T.; Welch, R. M.

1992-01-01

This paper investigates the automated detection of jet contrails using data from the Advanced Very High Resolution Radiometer. A preliminary algorithm subtracts the 11.8-micron image from the 10.8-micron image, creating a difference image on which contrails are enhanced. Then a three-stage algorithm searches the difference image for the nearly-straight line segments which characterize contrails. First, the algorithm searches for elevated, linear patterns called 'ridges'. Second, it applies a Hough transform to the detected ridges to locate nearly-straight lines. Third, the algorithm determines which of the nearly-straight lines are likely to be contrails. The paper applies this technique to several test scenes.

Estimating Contrail Climate Effects from Satellite Data

NASA Technical Reports Server (NTRS)

Minnis, Patrick; Duda, David P.; Palikonda, Rabindra; Bedka, Sarah T.; Boeke, Robyn; Khlopenkov, Konstantin; Chee, Thad; Bedka, Kristopher T.

2011-01-01

An automated contrail detection algorithm (CDA) is developed to exploit six of the infrared channels on the 1-km MODerate-resolution Imaging Spectroradiometer (MODIS) on the Terra and Aqua satellites. The CDA is refined and balanced using visual error analysis. It is applied to MODIS data taken by Terra and Aqua over the United States during 2006 and 2008. The results are consistent with flight track data, but differ markedly from earlier analyses. Contrail coverage is a factor of 4 less than other retrievals and the retrieved contrail optical depths and radiative forcing are smaller by approx.30%. The discrepancies appear to be due to the inability to detect wider, older contrails that comprise a significant amount of the contrail coverage. An example of applying the algorithm to MODIS data over the entire Northern Hemisphere is also presented. Overestimates of contrail coverage are apparent in some tropical regions. Methods for improving the algorithm are discussed and are to be implemented before analyzing large amounts of Northern Hemisphere data. The results should be valuable for guiding and validating climate models seeking to account for aviation effects on climate.

Contrails/cirrus optics and radiation

DOT National Transportation Integrated Search

2008-01-25

In this subject-specific white paper, we present a literature survey of past and current developments regarding the impact of contrails and contrail cirrus on the radiation field of the Earths atmosphere and climate. A number of recommendations fo...

DC-8 scanning lidar characterization of aircraft contrails and cirrus clouds

NASA Technical Reports Server (NTRS)

Nielsen, Norman B.; Uthe, Edward E. (Principal Investigator)

1996-01-01

A Subsonic Assessment (SASS) element of the overall Atmospheric Effects of Aviation Project (AEAP) was initiated by NASA to assess the atmospheric impact of subsonic aircraft. SRI was awarded a project to develop and test a scanning backscatter lidar for installation on the NASA DC-8 (year 1), participate in the Subsonic Aircraft: Contrail and Cloud Effects Special Study (SUCCESS) field program (year 2), and conduct a comprehensive analysis of field data (year 3). A scanning mirror pod attached to the DC-8 aircraft provides for scanning lidar observations ahead of the DC-8 and fixed-angle upward or downward observations. The lidar system installed within the DC-8 transmits 275 MJ at 1.06 gm wavelength or about 130 mJ at 1.06 and 0.53 gm simultaneously. Range-resolved aerosol backscatter is displayed in real time in terms of cloud/contrail spatial distributions. The objectives of the project are to map contrail/cloud vertical distributions ahead of DC-8; provide DC-8 guidance into enhanced scattering layers; document DC-8 flight path intersection of contrail and cloud geometries (in-situ measurement positions relative to cloud/contrail shape and an extension of in-situ measurements into the vertical -- integrated contrail/cloud properties); analyze contrail/cloud radiative properties with LIRAD (combined lidar and radiometry) technique; evaluate mean particle sizes of aircraft emissions from two-wavelength observations; study contrail/cloud interactions, diffusion, and mass decay/growth; and make observations in the near-field of aircraft engine emissions. The scanning mirror pod may also provide a scanning capability for other remote sensing instruments.

The GLOBE Contrail Protocol: Initial Analysis of Results

NASA Technical Reports Server (NTRS)

Chambers, Lin; Duda, David

2004-01-01

The GLOBE contrail protocol was launched in March 2003 to obtain surface observer reports of contrail occurrence to complement satellite and model studies underway at NASA Langley, among others. During the first year, more than 30,000 ground observations of contrails were submitted to GLOBE. An initial analysis comparing the GLOBE observations to weather prediction model results for relative humidity at flight altitudes is in progress. This paper reports on the findings to date from this effort.

The importance of the diurnal and annual cycle of air traffic for contrail radiative forcing.

PubMed

Stuber, Nicola; Forster, Piers; Rädel, Gaby; Shine, Keith

2006-06-15

Air traffic condensation trails, or contrails, are believed to have a net atmospheric warming effect, although one that is currently small compared to that induced by other sources of human emissions. However, the comparably large growth rate of air traffic requires an improved understanding of the resulting impact of aircraft radiative forcing on climate. Contrails have an effect on the Earth's energy balance similar to that of high thin ice clouds. Their trapping of outgoing longwave radiation emitted by the Earth and atmosphere (positive radiative forcing) is partly compensated by their reflection of incoming solar radiation (negative radiative forcing). On average, the longwave effect dominates and the net contrail radiative forcing is believed to be positive. Over daily and annual timescales, varying levels of air traffic, meteorological conditions, and solar insolation influence the net forcing effect of contrails. Here we determine the factors most important for contrail climate forcing using a sophisticated radiative transfer model for a site in southeast England, located in the entrance to the North Atlantic flight corridor. We find that night-time flights during winter (December to February) are responsible for most of the contrail radiative forcing. Night flights account for only 25 per cent of daily air traffic, but contribute 60 to 80 per cent of the contrail forcing. Further, winter flights account for only 22 per cent of annual air traffic, but contribute half of the annual mean forcing. These results suggest that flight rescheduling could help to minimize the climate impact of aviation.

NASA Alternative-Fuel Effects on Contrails and Cruise Emissions (ACCESS) Flight Experiments

NASA Astrophysics Data System (ADS)

Anderson, B. E.; Moore, R.; Beyersdorf, A. J.; Thornhill, K. L., II; Shook, M.; Winstead, E.; Ziemba, L. D.; Bulzan, D. L.; Brown, A.; Beaton, B.; Schlager, H.

2014-12-01

Although the emission performance of gas-turbine engines burning renewable aviation fuels have been thoroughly documented in recent ground-based studies, there is still great uncertainty regarding how the fuels effect aircraft exhaust composition and contrail formation at cruise altitudes. To fill this information gap, the NASA Aeronautics Research Mission Directorate sponsored the ACCESS flight series to make detailed measurements of trace gases, aerosols and ice particles in the near-field behind the NASA DC-8 aircraft as it burned either standard petroleum-based fuel of varying sulfur content or a 50:50 blend of standard fuel and a hydro-treated esters and fatty acid (HEFA) jet fuel produced from camelina plant oil. ACCESS 1, conducted in spring 2013 near Palmdale CA, focused on refining flight plans and sampling techniques and used the instrumented NASA Langley HU-25 aircraft to document DC-8 emissions and contrails on five separate flights of ~2 hour duration. ACCESS 2, conducted from Palmdale in May 2014, engaged partners from the Deutsches Zentrum für Luft- und Raumfahrt (DLR) and National Research Council-Canada to provide additional scientific expertise and sampling aircraft (Falcon 20 and CT-133, respectively) with more extensive trace gas, particle, or air motion measurement capability. Eight, muliti-aircraft research flights of 2 to 4 hour duration were conducted to document the emissions and contrail properties of the DC-8 as it 1) burned low sulfur Jet A, high sulfur Jet A or low sulfur Jet A/HEFA blend, 2) flew at altitudes between 6 and 11 km, and 3) operated its engines at three different fuel flow rates. This presentation further describes the ACCESS flight experiments, examines fuel type and thrust setting impacts on engine emissions, and compares cruise-altitude observations with similar data acquired in ground-test venues.

DC-8 Scanning Lidar Characterization of Aircraft Contrails and Cirrus Clouds

NASA Technical Reports Server (NTRS)

Uthe, Edward E.; Nielsen, Norman B.; Oseberg, Terje E.

1998-01-01

An angular-scanning large-aperture (36 cm) backscatter lidar was developed and deployed on the NASA DC-8 research aircraft as part of the SUCCESS (Subsonic Aircraft: Contrail and Cloud Effects Special Study) program. The lidar viewing direction could be scanned continuously during aircraft flight from vertically upward to forward to vertically downward, or the viewing could be at fixed angles. Real-time pictorial displays generated from the lidar signatures were broadcast on the DC-8 video network and used to locate clouds and contrails above, ahead of, and below the DC-8 to depict their spatial structure and to help select DC-8 altitudes for achieving optimum sampling by onboard in situ sensors. Several lidar receiver systems and real-time data displays were evaluated to help extend in situ data into vertical dimensions and to help establish possible lidar configurations and applications on future missions. Digital lidar signatures were recorded on 8 mm Exabyte tape and generated real-time displays were recorded on 8mm video tape. The digital records were transcribed in a common format to compact disks to facilitate data analysis and delivery to SUCCESS participants. Data selected from the real-time display video recordings were processed for publication-quality displays incorporating several standard lidar data corrections. Data examples are presented that illustrate: (1) correlation with particulate, gas, and radiometric measurements made by onboard sensors, (2) discrimination and identification between contrails observed by onboard sensors, (3) high-altitude (13 km) scattering layer that exhibits greatly enhanced vertical backscatter relative to off-vertical backscatter, and (4) mapping of vertical distributions of individual precipitating ice crystals and their capture by cloud layers. An angular scan plotting program was developed that accounts for DC-8 pitch and velocity.

Alternative-Fuel Effects on Contrails & Cruise Emissions (ACCESS-2) Flight Experiment

NASA Technical Reports Server (NTRS)

Anderson, Bruce E.

2015-01-01

Although the emission performance of gas-turbine engines burning renewable aviation fuels have been thoroughly documented in recent ground-based studies, there is still great uncertainty regarding how the fuels effect aircraft exhaust composition and contrail formation at cruise altitudes. To fill this information gap, the NASA Aeronautics Research Mission Directorate sponsored the ACCESS flight series to make detailed measurements of trace gases, aerosols and ice particles in the near-field behind the NASA DC-8 aircraft as it burned either standard petroleum-based fuel of varying sulfur content or a 50:50 blend of standard fuel and a hydro-treated esters and fatty acid (HEFA) jet fuel produced from camelina plant oil. ACCESS 1, conducted in spring 2013 near Palmdale CA, focused on refining flight plans and sampling techniques and used the instrumented NASA Langley HU-25 aircraft to document DC-8 emissions and contrails on five separate flights of approx.2 hour duration. ACCESS 2, conducted from Palmdale in May 2014, engaged partners from the Deutsches Zentrum fuer Luft- und Raumfahrt (DLR) and National Research Council-Canada to provide additional scientific expertise and sampling aircraft (Falcon 20 and CT-133, respectively) with more extensive trace gas, particle, or air motion measurement capability. Eight, muliti-aircraft research flights of 2 to 4 hour duration were conducted to document the emissions and contrail properties of the DC-8 as it 1) burned low sulfur Jet A, high sulfur Jet A or low sulfur Jet A/HEFA blend, 2) flew at altitudes between 6 and 11 km, and 3) operated its engines at three different fuel flow rates. This presentation further describes the ACCESS flight experiments, examines fuel type and thrust setting impacts on engine emissions, and compares cruise-altitude observations with similar data acquired in ground tests.

Do contrails significantly reduce daily temperature range?

NASA Astrophysics Data System (ADS)

Hong, Gang; Yang, Ping; Minnis, Patrick; Hu, Yong X.; North, Gerald

2008-12-01

One of the most visible anthropogenic phenomena in the atmosphere is the occurrence of contrails. The direct effects of contrails on surface temperature are investigated on the basis of the data sets for the cloud cover and surface temperature over the conterminous United States for the period 1971-2001. It is shown that the increase of the average daily temperature range (DTR) over the United States during the three-day grounding period of 11-14 September 2001 cannot be attributed to the absence of contrails, a subject was debated in several previous studies. The present analysis suggests that the DTR is attributed to the change of low cloudiness.

The GLOBE Earth Day 2004 Contrail Count-a-Thon

NASA Technical Reports Server (NTRS)

Chambers, Lin H.; Cole, Julia

2004-01-01

Early in 2004 the GLOBE Science team suggested a contrail count activity to celebrate Earth Day 2004, which was held this year on April 22nd in the United States and some other countries around the world. The GLOBE contrail team embraced this idea and developed a simplified data collection sheet for this special project. Information about the event was shared through the GLOBE site, UCAR and NASA press releases, the NASA portal (http://www.nasa.gov) and the CERES S'COOL Project (http://scool.larc.nasa.gov). On Earth Day, about 120 observations were received through the GLOBE Contrail Count-a-Thon website, about 70 contrail observations were received through regular GLOBE data reporting, and 19 contrail observations were received through regular S'COOL data reporting. Only observations between 11:00 and 13:00 local time were included in the Count-a-Thon. The event was reported in the Boulder Daily Camera beforehand and in the Oregon Register-Guard after the fact. It was also reported on National Public Radio s Day-to-Day show; whose host even submitted an observation. This poster discusses the Count-a-Thon experience and reports the results.

Carbon balance of China constrained by CONTRAIL aircraft CO2 measurements

NASA Astrophysics Data System (ADS)

Jiang, F.; Wang, H. M.; Chen, J. M.; Machida, T.; Zhou, L. X.; Ju, W. M.; Matsueda, H.; Sawa, Y.

2014-03-01

Terrestrial CO2 flux estimates in China using atmospheric inversion method are beset with considerable uncertainties because very few atmospheric CO2 concentration measurements are available. In order to improve these estimates, nested atmospheric CO2 inversion during 2002-2008 is performed in this study using passenger aircraft-based CO2 measurements over Eurasia from the Comprehensive Observation Network for Trace gases by Airliner (CONTRAIL) project. The inversion system includes 43 regions with a focus on China, and is based on the Bayesian synthesis approach and the TM5 transport model. The terrestrial ecosystem carbon flux modeled by the BEPS model and the ocean exchange simulated by the OPA-PISCES-T model are considered as the prior fluxes. The impacts of CONTRAIL CO2 data on inverted China terrestrial carbon fluxes are quantified, the improvement of the inverted fluxes after adding CONTRAIL CO2 data are rationed against climate factors and evaluated by comparing the simulated atmospheric CO2 concentrations with three independent surface CO2 measurements in China. Results show that with the addition of CONTRAIL CO2 data, the inverted carbon sink in China increases while those in South and Southeast Asia decrease. Meanwhile, the posterior uncertainties over these regions are all reduced. CONTRAIL CO2 data also have a large effect on the inter-annual variation of carbon sinks in China, leading to a better correlation between the carbon sink and the annual mean climate factors. Evaluations against the CO2 measurements at three sites in China also show that the CONTRAIL CO2 measurements have improved the inversion results.

Carbon balance of China constrained by CONTRAIL aircraft CO2 measurements

NASA Astrophysics Data System (ADS)

Jiang, F.; Wang, H. M.; Chen, J. M.; Machida, T.; Zhou, L. X.; Ju, W. M.; Matsueda, H.; Sawa, Y.

2014-09-01

Terrestrial carbon dioxide (CO2) flux estimates in China using atmospheric inversion method are beset with considerable uncertainties because very few atmospheric CO2 concentration measurements are available. In order to improve these estimates, nested atmospheric CO2 inversion during 2002-2008 is performed in this study using passenger aircraft-based CO2 measurements over Eurasia from the Comprehensive Observation Network for Trace gases by Airliner (CONTRAIL) project. The inversion system includes 43 regions with a focus on China, and is based on the Bayesian synthesis approach and the TM5 transport model. The terrestrial ecosystem carbon flux modeled by the Boreal Ecosystems Productivity Simulator (BEPS) model and the ocean exchange simulated by the OPA-PISCES-T model are considered as the prior fluxes. The impacts of CONTRAIL CO2 data on inverted China terrestrial carbon fluxes are quantified, the improvement of the inverted fluxes after adding CONTRAIL CO2 data are rationed against climate factors and evaluated by comparing the simulated atmospheric CO2 concentrations with three independent surface CO2 measurements in China. Results show that with the addition of CONTRAIL CO2 data, the inverted carbon sink in China increases while those in South and Southeast Asia decrease. Meanwhile, the posterior uncertainties over these regions are all reduced (2-12%). CONTRAIL CO2 data also have a large effect on the inter-annual variation of carbon sinks in China, leading to a better correlation between the carbon sink and the annual mean climate factors. Evaluations against the CO2 measurements at three sites in China also show that the CONTRAIL CO2 measurements may have improved the inversion results.

Simulation and Optimization Methods for Assessing the Impact of Aviation Operations on the Environment

NASA Technical Reports Server (NTRS)

Sridhar, Banavar; Chen, Neil; Ng, Hok K.

2010-01-01

There is increased awareness of anthropogenic factors affecting climate change and urgency to slow the negative impact. Greenhouse gases, oxides of Nitrogen and contrails resulting from aviation affect the climate in different and uncertain ways. This paper develops a flexible simulation and optimization software architecture to study the trade-offs involved in reducing emissions. The software environment is used to conduct analysis of two approaches for avoiding contrails using the concepts of contrail frequency index and optimal avoidance trajectories.

Variability of the contrail radiative forcing due to crystal shape

NASA Astrophysics Data System (ADS)

Markowicz, K. M.; Witek, M. L.

2011-12-01

The aim of this study is to examine the influence of particles' shape and particles' optical properties on the contrail radiative forcing. Contrail optical properties in the shortwave and longwave range are derived using a ray-tracing geometric method and the discrete dipole approximation method, respectively. Both methods present good correspondence of the single scattering albedo and the asymmetry parameter in a transition range (3-7μm). We compare optical properties defined following simple 10 crystals habits randomly oriented: hexagonal plates, hexagonal columns with different aspect ratio, and spherical. There are substantial differences in single scattering properties between ten crystal models investigated here (e.g. hexagonal columns and plates with different aspect ratios, spherical particles). The single scattering albedo and the asymmetry parameter both vary up to 0.1 between various crystal shapes. Radiative forcing calculations were performed using a model which includes an interface between the state-of-the-art radiative transfer model Fu-Liou and databases containing optical properties of the atmosphere and surface reflectance and emissivity. This interface allows to determine radiative fluxes in the atmosphere and to estimate the contrail radiative forcing for clear- and all-sky (including natural clouds) conditions for various crystal shapes. The Fu-Liou code is fast and therefore it is suitable for computing radiative forcing on a global scale. At the same time it has sufficiently good accuracy for such global applications. A noticeable weakness of the Fu-Liou code is that it does not take into account the 3D radiative effects, e.g. cloud shading and horizontal. Radiative transfer model calculations were performed at horizontal resolution of 5x5 degree and time resolution of 20 min during day and 3 h during night. In order to calculate a geographic distribution of the global and annual mean contrail radiative forcing, the contrail cover must be determined. Two cases are discussed here: a 1% homogeneous contrail cover and the contrail cover provided by Rädel and Shine (2008). In the second distribution case, a more realistic contrail cover is taken into account. This model combines the AERO2K flight inventory with meteorological data and normalizes it with respect to the contrail cover derived from satellite observations. Simulations performed by the Fu-Liou model show significant variability of the shortwave, longwave, and net radiative forcing with crystal shape. The nonspherical crystals have smaller net forcing in contrary to spherical particles. The differences in net radiative forcing between optical models reach up to 50%. The hexagonal column and hexagonal plate particles show the smallest net radiative forcing while the largest forcing is obtained for the spheres. The global and annual mean shortwave, longwave, and net contrail radiative forcing, average over all crystal models and assuming an optical depth of 0.3 at visible wavelengths, is -5.7, 16.8, and 11.1 mW/m2, respectively. A ratio of the radiative forcings' standard deviation to the mean value, derived using 10 different ice particle models, is about 0.2 for the shortwave, 0.14 for the longwave, and 0.23 for the net radiation.

Contrail Cirrus Forecasts for the ML-CIRRUS Experiment and Some Comparison Results

NASA Astrophysics Data System (ADS)

Schumann, Ulrich; Graf, Kaspar; Bugliaro, Luca; Dörnbrack, Andreas; Giez, Andreas; Jurkat, Tina; Kaufmann, Stefan; Krämer, Martina; Minikin, Andreas; Schäfler, Andreas; Voigt, Christiane; Wirth, Martin; Zahn, Andreas; Ziereis, Helmut

2015-04-01

Model simulations with the contrail cirrus prediction model CoCiP driven by numerical weather prediction (NWP) data provided from the European Centre for Medium Range Forecasts (ECMWF) and global aircraft waypoint data show a mean computed cover (for optical depth larger than 0.1) of 0.23% globally, and 5.4% over mid Europe (Schumann and Graf, JGR, 2013). The computed mean longwave radiative forcing (RF) reaches 3 W m-2 over mid Europe (10°W-20°E and 40°N-55°N), and 0.13 W m-2 globally. The global net RF is about 40-60% smaller because of compensating shortwave cooling induced by contrails during daytime. The results depend on several model details such as the number of ice particles forming from aircraft soot emissions, the contrail plume dispersion, ice particle sedimentation etc., all influencing contrail life time and their optical properties. The quantitative results depend also strongly on ambient relative humidity, vertical motion and on ice water content of other cirrus predicted by the NWP model. In order to test and possibly improve this and other contrail models, high-quality observations are needed to which multi-parameter model output can be compared. The Mid-Latitude Cirrus Experiment ML-CIRRUS was performed (see C. Voigt et al., this conference) with a suite of in-situ and Lidar instruments for airborne measurements on the research aircraft HALO. Before and during the mission, CoCiP was run daily to provide 3-days forecasts of contrail cover using operational ECMWF forecasts and historical traffic data. CoCiP forecast output was made available in an internet tool twice a day for experiment planning. The one-day and two-day contrail forecasts often showed only small differences. Still, most recent forecasts and detailed satellite observations results were transmitted via satellite link to the crew for onboard campaign optimization. After the campaign, a data base of realistic air traffic data has been setup from various sources, and CoCiP was rerun with improved ECMWF-NWP data (at one-hour time resolution). The model results are included in the HALO mission data bank, and the results are available for comparison to in-situ data. The data are useful for identifying aircraft and other sources for measured air properties. The joint analysis of observations and model result has basically just started. Preliminary results from comparisons with lidar-measured extinction profiles, in-situ measured humidity, nitrogen oxides, and aerosol and ice particle concentrations, and with meteorological observations (wind, temperature etc.) illustrate the expected gain in insight. The contrail forecasts have been checked by comparison to available data including satellite data and HALO observations. During the campaign, it became obvious that predicted contrail cirrus cover compared qualitatively mostly well with what was found when HALO reached predicted cirrus regions. From the analysis of the measured data, some examples of significant correlation between model results and observations have been found. However, the quantitative agreement is not uniform. As expected, nature is far more variable than a model can predict. The observed optical properties of cirrus and contrails vary far more in time and space than predicted. Local values were often far higher or lower than mean values. A one-to-one correlation between local observations and model results is not to be expected. This inhomogeneity may have consequences for the climate impact of aviation induced cloud changes.

NASA Alternative Aviation Fuel Research

NASA Astrophysics Data System (ADS)

Anderson, B. E.; Beyersdorf, A. J.; Thornhill, K. L., II; Moore, R.; Shook, M.; Winstead, E.; Ziemba, L. D.; Crumeyrolle, S.

2015-12-01

We present an overview of research conducted by NASA Aeronautics Research Mission Directorate to evaluate the performance and emissions of "drop-in" alternative jet fuels, highlighting experiment design and results from the Alternative Aviation Fuel Experiments (AAFEX-I & -II) and Alternative Fuel-Effects on Contrails and Cruise Emissions flight series (ACCESS-I & II). These projects included almost 100 hours of sampling exhaust emissions from the NASA DC-8 aircraft in both ground and airborne operation and at idle to takeoff thrust settings. Tested fuels included Fischer-Tropsch (FT) synthetic kerosenes manufactured from coal and natural-gas feedstocks; Hydro-treated Esters and Fatty-Acids (HEFA) fuels made from beef-tallow and camelina-plant oil; and 50:50 blends of these alternative fuels with Jet A. Experiments were also conducted with FT and Jet A fuels doped with tetrahydrothiophene to examine the effects of fuel sulfur on volatile aerosol and contrail formation and microphysical properties. Results indicate that although the absence of aromatic compounds in the alternative fuels caused DC-8 fuel-system leaks, the fuels did not compromise engine performance or combustion efficiency. And whereas the alternative fuels produced only slightly different gas-phase emissions, dramatic reductions in non-volatile particulate matter (nvPM) emissions were observed when burning the pure alternative fuels, particularly at low thrust settings where particle number and mass emissions were an order of magnitude lower than measured from standard jet fuel combustion; 50:50 blends of Jet A and alternative fuels typically reduced nvPM emissions by ~50% across all thrust settings. Alternative fuels with the highest hydrogen content produced the greatest nvPM reductions. For Jet A and fuel blends, nvPM emissions were positively correlated with fuel aromatic and naphthalene content. Fuel sulfur content regulated nucleation mode aerosol number and mass concentrations within aging exhaust plumes, but did not clearly impact contrail formation or microphysics.

Variations of GHGs from the lower-troposphere to the UT/LS revealed by two Japanese regular aircraft observation programs

NASA Astrophysics Data System (ADS)

Niwa, Yosuke; Machida, Toshinobu; Sawa, Yousuke; Tsuboi, Kazuhiro; Matsueda, Hidekazu; Imasu, Ryoichi

2014-05-01

A Japan-centered observation network consisting of two regular aircraft programs have revealed the greenhouse gases variations from the lower-troposphere to the upper-troposphere/lower-stratosphere (UT/LS) regions. In the Comprehensive Observation Network for Trace gases by Airliner (CONTRAIL) project, in-situ continuous measurement equipment (CME) onboard commercial passenger aircraft world-widely observes CO2 profiles in vertical over tens of airports and in horizontal in the UT/LS regions. The CONTRAIL-CME has revealed three-dimensional structure of the global CO2 distribution and has exposed significant inter-hemispheric transport of CO2 through the upper-troposphere. In inverse modeling, the CME data have provided strong constraints on CO2 flux estimation especially for the Asian tropics. Automatic flask air sampling equipment (ASE) is also onboard the CONTRAIL aircraft and has been observing CO2 mixing ratios as well as those of methane, carbon monoxide, nitrous oxide and other trace species in the upper-troposphere between Japan and Australia. The observation period of the ASE has reached 20 years. In recent years, the ASE program has extended to the northern subarctic UT/LS region and has given an insight of transport mechanisms in the UT/LS by observing seasonal GHGs variations. In the other aircraft observation program by Japan Meteorological Agency, variations of GHGs have been observed by flask-sampling onboard a C-130H aircraft horizontally in the mid-troposphere over the western North Pacific as well as vertically over Minamitorishima-Island. The C-130H aircraft has persistently observed high mixing ratios of CH4 in the mid-troposphere, which seems to be originated from fossil fuel combustion throughout the year as well as from biogenic sources during summer in the Asian regions. Those above aircraft observation programs have a significant role for constraining GHGs flux estimates by filling the data gap of the existing surface measurement network specifically in the regions of Asia and the western North Pacific.

A multi-approach to the optical depth of a contrail cirrus cluster

NASA Astrophysics Data System (ADS)

Vazquez-Navarro, Margarita; Bugliaro, Luca; Schumann, Ulrich; Strandgren, Johan; Wirth, Martin; Voigt, Christiane

2017-04-01

Amongst the individual aviation emissions, contrail cirrus contribute the largest fraction to the aviation effects on climate. To investigate the optical depth from contrail cirrus, we selected a cirrus and contrail cloud outbreak on the 10th April 2014 between the North Sea and Switzerland detected during the ML-CIRRUS experiment (Voigt et al., 2017). The outbreak was not forecast by weather prediction models. We describe its origin and evolution using a combination of in-situ measurements, remote sensing approaches and contrail prediction model prognosis. The in-situ and lidar measurements were carried out with the HALO aircraft, where the cirrus was first identified. Model predictions from the contrail prediction model CoCiP (Schumann et al., 2012) point to an anthropogenic origin. The satellite pictures from the SEVIRI imager on MSG combined with the use of a contrail cluster tracking algorithm enable the automatic assessment of the origin, displacement and growth of the cloud and the correct labeling of cluster pixels. The evolution of the optical depth and particle size of the selected cluster pixels were derived using the CiPS algorithm, a neural network primarily based on SEVIRI images. The CoCiP forecast of the cluster compared to the actual cluster tracking show that the model correctly predicts the occurrence of the cluster and its advection direction although the cluster spreads faster than simulated. The optical depth derived from CiPS and from the airborne high spectral resolution lidar WALES are compared and show a remarkably good agreement. This confirms that the new CiPS algorithm is a very powerful tool for the assessment of the optical depth of even optically thinner cirrus clouds. References: Schumann, U.: A contrail cirrus prediction model, Geosci. Model Dev., 5, 543-580, doi: 10.5194/gmd-5-543-2012, 2012. Voigt, C., Schumann, U., Minikin, A., Abdelmonem, A., Afchine, A., Borrmann, S., Boettcher, M., Buchholz, B., Bugliaro, L., Costa, A., Curtius, J., Dollner, M., Dörnbrack, A., Dreiling, V., Ebert, V., Ehrlich, A., Fix, A., Forster, L., Frank, F., Fütterer, D., Giez, A., Graf, K., Grooß, J.-U., Groß, S., Heimerl, K., Heinold, B., Hüneke, T., Järvinen, E., Jurkat, T., Kaufmann, S., Kenntner, M., Klingebiel, M., Klimach, T., Kohl, R., Krämer, M., Krisna, T. C., Luebke, A., Mayer, B., Mertes, S., Molleker, S., Petzold, A., Pfeilsticker, K., Port, M., Rapp, M., Reutter, P., Rolf, C., Rose, D., Sauer, D., Schäfler, A., Schlage, R., Schnaiter, M., Schneider, J., Spelten, N., Spichtinger, P., Stock, P., Walser, A., Weigel, R., Weinzierl, B., Wendisch, M., Werner, F., Wernli, H., Wirth, M., Zahn, A., Ziereis, H., and Zöger, M.: ML-CIRRUS - The airborne experiment on natural cirrus and contrail cirrus with the high-altitude long-range research aircraft HALO, Bull. Amer. Meteorol. Soc., in press, doi: 10.1175/BAMS-D-15-00213.1, 2017.

Advanced Energetics for Aeronautical Applications. Volume II

NASA Technical Reports Server (NTRS)

Alexander, David S.

2005-01-01

NASA has identified water vapor emission into the upper atmosphere from commercial transport aircraft, particularly as it relates to the formation of persistent contrails, as a potential environmental problem. Since 1999, MSE has been working with NASA-LaRC to investigate the concept of a transport-size emissionless aircraft fueled with liquid hydrogen combined with other possible breakthrough technologies. The goal of the project is to significantly advance air transportation in the next decade and beyond. The power and propulsion (P/P) system currently being studied would be based on hydrogen fuel cells (HFCs) powering electric motors, which drive fans for propulsion. The liquid water reaction product is retained onboard the aircraft until a flight mission is completed. As of now, NASA-LaRC and MSE have identified P/P system components that, according to the high-level analysis conducted to date, are light enough to make the emissionless aircraft concept feasible. Calculated maximum aircraft ranges (within a maximum weight constraint) and other performance predictions are included in this report. This report also includes current information on advanced energy-related technologies, which are still being researched, as well as breakthrough physics concepts that may be applicable for advanced energetics and aerospace propulsion in the future.

Contrails of Small and Very Large Optical Depth

NASA Technical Reports Server (NTRS)

Atlas, David; Wang, Zhien

2010-01-01

This work deals with two kinds of contrails. The first comprises a large number of optically thin contrails near the tropopause. They are mapped geographically using a lidar to obtain their height and a camera to obtain azimuth and elevation. These high-resolution maps provide the local contrail geometry and the amount of optically clear atmosphere. The second kind is a single trail of unprecedentedly large optical thickness that occurs at a lower height. The latter was observed fortuitously when an aircraft moving along the wind direction passed over the lidar, thus providing measurements for more than 3 h and an equivalent distance of 620 km. It was also observed by Geostationary Operational Environmental Satellite (GOES) sensors. The lidar measured an optical depth of 2.3. The corresponding extinction coefficient of 0.023 per kilometer and ice water content of 0.063 grams per cubic meter are close to the maximum values found for midlatitude cirrus. The associated large radar reflectivity compares to that measured by ultrasensitive radar, thus providing support for the reality of the large optical depth.

The time-dependence of the defective nature of ice Ic (cubic ice) and its implications for atmospheric science

NASA Astrophysics Data System (ADS)

Sippel, Christian; Koza, Michael M.; Hansen, Thomas C.; Kuhs, Werner F.

2010-05-01

The possible atmospheric implication of ice Ic (cubic ice) has already been suggested some time ago in the context of snow crystal formation [1]. New findings from air-borne measurements in cirrus clouds and contrails have put ice Ic into the focus of interest to understand the so-called "supersaturation puzzle" [2,3,4]. Our recent microstructural work on ice Ic [5,6] appears to be highly relevant in this context. We have found that ice Ic is characterized by a complex stacking fault pattern, which changes as a function of temperature as well as time. Indeed, from our own [7] and other group's work [8] one knows that (in contrast to earlier believe) ice Ic can form up to temperatures at least as high as 240K - thus in the relevant range for cirrus clouds. We have good preliminary evidence that the "cubicity" (which can be related to stacking fault probabilities) as well as the particle size of ice Ic are the relevant parameters for this correlation. The "cubicity" of stacking faulty ice Ic (established by diffraction) correlates nicely with the increased supersaturation at decreasing temperatures observed in cirrus clouds and contrails, a fact, which may be considered as further evidence for the presence of ice Ic. Recently, we have studied the time-dependency of the changes in both "cubicity" and particle size at various temperatures of relevance for cirrus clouds and contrails by in-situ neutron powder diffraction. The timescales over which changes occur (several to many hours) are similar to the life-time of cirrus clouds and contrails and suggest that the supersaturation situation may change within this time span in the natural environment too. Some accompanying results obtained by cryo-SEM (scanning electron microscopy) work will also be presented and suggest that stacking-faulty ice Ic has kinky surfaces providing many more active centres for heterogeneous reactions on the surface than in the usually assumed stable hexagonal form of ice Ih with its rather flat low-indexed crystal faces. [1] T Kobayashi & T Kuroda (1987) Snow Crystals. In: Morphology of Crystals (ed. I Sunagawa), Terra Scientific Publishing, Tokyo, pp.649-743. [2] RS Gao & 19 other authors (2004) Evidence that nitric acid increases relative humidity in low-temperature cirrus clouds. Science 303, 516-520. [3] T Peter, C Marcolli, P Spichtinger, T Corti, MC Baker & T Koop (2006) When dry air is too humid. Science 314, 1399-1402. [4] JE Shilling, MA Tolbert, OB Toon, EJ Jensen, BJ Murray & AK Bertram (2006) Measurements of the vapor pressure of cubic ice and their implications for atmospheric ice clouds. Geophys.Res.Lett. 33, 026671. [5] TC Hansen, MM Koza & WF Kuhs (2008) Formation and annealing of cubic ice: I Modelling of stacking faults. J.Phys.Cond.Matt. 20, 285104. [6] TC Hansen, MM Koza, P Lindner & WF Kuhs (2008) Formation and annealing of cubic ice: II. Kinetic study. J.Phys.Cond.Matt. 20, 285105. [7] WF Kuhs, G Genov, DK Staykova & AN Salamatin, T Hansen (2004) Ice perfection and the onset of anomalous preservation of gas hydrates. Phys.Chem.Chem.Phys. 6, 4917-4920. [8] BJ Murray, DA Knopf & AK Bertram (2005) The formation of cubic ice under conditions relevant to Earth's atmosphere. Nature 434, 292-205.

The Role of Turbulence in Chemical and Dynamical Processes in the Near-Field Wake of Subsonic Aircraft

NASA Technical Reports Server (NTRS)

Lewellen, D. C.; Lewellen, W. Steve

2002-01-01

During this grant, covering the period from September 1998 to December 2001, we continued the investigation of the role of turbulent mixing in the wake of subsonic aircraft initiated in 1994 for NASA's Atmospheric Effects of Aviation Project. The goal of the research has been to provide sufficient understanding and quantitative analytical capability to assess the dynamical, chemical, and microphysical interactions in the near-field wake that have the greatest potential to influence the global atmospheric impact of the projected fleet of subsonic aircraft. Through large-eddy simulations we have shown that turbulence in the early wake dynamics can have a strong effect on both the ice microphysics of contrail evolution and on wake chemistry. The wake vortex dynamics are the primary determinant of the vertical extent of the contrail; this together with the local wind shear largely determines the horizontal extent. The fraction of the initial ice crystals surviving the wake vortex dynamics, their spatial distribution, and the ice mass distribution are all sensitive to the aircraft type, assumed initial ice crystal number, and ambient humidity and turbulence conditions. Our model indicates that there is a significant range of conditions for which a smaller aircraft such as a B737 produces as significant a persistent contrail as a larger aircraft such as a B747, even though the latter consumes almost five times as much fuel. Large-eddy simulations of the near wake of a B757 provided a fine-grained chemical-dynamical representation of simplified NOx - HOx chemistry in wakes of ages from a few seconds to several minutes. By sampling the simulated data in a manner similar to that of in situ aircraft measurements it was possible to provide a likely explanation for a puzzle uncovered in the 1996 SUCCESS flight measurements of OH and HO2 The results illustrate the importance of considering fluid dynamics effects in interpreting chemistry results when mixing rates and species fluctuations are large, and demonstrate the feasibility of using 3D unsteady LES with coupled chemistry to study such phenomena.

Optical Properties of Ice Particles in Young Contrails

NASA Technical Reports Server (NTRS)

Hong, Gang; Feng, Qian; Yang, Ping; Kattawar, George; Minnis, Patrick; Hu, Yong X.

2008-01-01

The single-scattering properties of four types of ice crystals (pure ice crystals, ice crystals with an internal mixture of ice and black carbon, ice crystals coated with black carbon, and soot coated with ice) in young contrails are investigated at wavelengths 0.65 and 2.13 micrometers using Mie codes from coated spheres. The four types of ice crystals have distinct differences in their single-scattering properties because of the embedded black carbon. The bulk scattering properties of young contrails consisting of the four types of ice crystals are further investigated by averaging their single-scattering properties over a typical ice particle size distribution found in young contrails. The effect of the radiative properties of the four types of ice particles on the Stokes parameters I, Q, U, and V is also investigated for different viewing zenith angles and relative azimuth angles with a solar zenith angle of 30 degrees using a vector radiative transfer model based on the adding-doubling technique. The Stokes parameters at a wavelength of 0.65 micrometers show pronounced differences for the four types of ice crystals. Those at a wavelength of 2.13 micrometers show similar variations with the viewing zenith angle and relative azimuth angle, but their values are noticeably different.

Optical scattering and microphysical properties of subvisual cirrus clouds, and climatic implications

NASA Technical Reports Server (NTRS)

Sassen, Kenneth; Griffin, Michael K.; Dodd, Gregory C.

1989-01-01

The optical and microphysical properties of subvisual cirrus clouds are derived from ground-based polarization lidar, shortwave radiation flux, and solar corona measurements of two approximately 0.75 km deep cirrus located near the tropopause. The first cloud produced no visual manifestations under excellent viewing conditions, and the second appeared to be a persistent aircraft contrail that was generally visible except in the zenith direction. Average lidar linear depolarization ratios and volume backscatter coefficients for the two clouds were 0.19 and 0.35, and 0.6 x 10 to the -3 and 1.4 x 10 to the -3 /km sr, respectively. It is estimated that the zenith-subvisual cirrus contained ice crystals of 25-micron effective diameter at a mean concentration of 25/1 and ice mass content of 0.2 mg/cu m. The threshold cloud optical thickness for visual-versus-invisible cirrus, derived from both broadband shortwave flux and 0.694 micrometer lidar data, is found to be tau sub c approx equal 0.03. Such tau values are comparable to those of 5 to 10 km deep stratospheric aerosol clouds of volcanic origin and polar stratospheric clouds, which are episodic in nature. Hence, we conclude that if these clouds are a fairly common feature of the upper troposphere, as recent SAGE satellite measurements would suggest, then the impact of natural and contrail subvisual cirrus on the planet's radiation balance may be relatively significant.

Optical scattering and microphysical properties of subvisual cirrus clouds, and climatic implications

NASA Technical Reports Server (NTRS)

Sassen, Kenneth; Griffin, Michael K.; Dodd, Gregory C.

1988-01-01

The optical and microphysical properties of subvisual cirrus clouds are derived from ground-based polarization lidar, shortwave radiation flux, and solar corona measurements of two approximately 0.75 km deep cirrus located near the tropopause. The first cloud produced no visual manifestations under excellent viewing conditions, and the second appeared to be a persistent aircraft contrail that was generally visible except in the zenith direction. Average lidar linear depolarization ratios and volume backscatter coefficients for the two clouds were 0.19 and 0.35, and 0.6x10 to the -3 and 1.4x10 to the -3 /km sr, respectively. It is estimated that the zenith-subvisual cirrus contained ice crystals of 25 micron effective diameter at a mean concentration of 25/l and ice mass content of 0.2 mg/cu m. The threshold cloud optical thickness for visual-versus-invisible cirrus, derived from both broadband shortwave flux and 0.694 micrometer lidar data, is found to be tau sub c approx equal 0.03. Such tau values are comparable to those of 5 to 10 km deep stratospheric aerosol clouds of volcanic origin and polar stratospheric clouds, which are episodic in nature. Hence, we conclude that if these clouds are a fairly common feature of the upper troposphere, as recent SAGE satellite measurements would suggest, then the impact of natural and contrail subvisual cirrus on the planet's radiation balance may be relatively significant.

Aviation effects on already-existing cirrus clouds

PubMed Central

Tesche, Matthias; Achtert, Peggy; Glantz, Paul; Noone, Kevin J.

2016-01-01

Determining the effects of the formation of contrails within natural cirrus clouds has proven to be challenging. Quantifying any such effects is necessary if we are to properly account for the influence of aviation on climate. Here we quantify the effect of aircraft on the optical thickness of already-existing cirrus clouds by matching actual aircraft flight tracks to satellite lidar measurements. We show that there is a systematic, statistically significant increase in normalized cirrus cloud optical thickness inside mid-latitude flight tracks compared with adjacent areas immediately outside the tracks. PMID:27327838

Aviation effects on already-existing cirrus clouds.

PubMed

Tesche, Matthias; Achtert, Peggy; Glantz, Paul; Noone, Kevin J

2016-06-21

Determining the effects of the formation of contrails within natural cirrus clouds has proven to be challenging. Quantifying any such effects is necessary if we are to properly account for the influence of aviation on climate. Here we quantify the effect of aircraft on the optical thickness of already-existing cirrus clouds by matching actual aircraft flight tracks to satellite lidar measurements. We show that there is a systematic, statistically significant increase in normalized cirrus cloud optical thickness inside mid-latitude flight tracks compared with adjacent areas immediately outside the tracks.

Climate impact of anthropogenic aerosols on cirrus clouds

NASA Astrophysics Data System (ADS)

Penner, J.; Zhou, C.

2017-12-01

Cirrus clouds have a net warming effect on the atmosphere and cover about 30% of the Earth's area. Aerosol particles initiate ice formation in the upper troposphere through modes of action that include homogeneous freezing of solution droplets, heterogeneous nucleation on solid particles immersed in a solution, and deposition nucleation of vapor onto solid particles. However, the efficacy with which particles act to form cirrus particles in a model depends on the representation of updrafts. Here, we use a representation of updrafts based on observations of gravity waves, and follow ice formation/evaporation during both updrafts and downdrafts. We examine the possible change in ice number concentration from anthropogenic soot originating from surface sources of fossil fuel and biomass burning and from aircraft particles that have previously formed ice in contrails. Results show that fossil fuel and biomass burning soot aerosols with this version exert a radiative forcing of -0.15±0.02 Wm-2 while aircraft aerosols that have been pre-activated within contrails exert a forcing of -0.20±0.06 Wm-2, but it is possible to decrease these estimates of forcing if a larger fraction of dust particles act as heterogeneous ice nuclei. In addition aircraft aerosols may warm the climate if a large fraction of these particles act as ice nuclei. The magnitude of the forcing in cirrus clouds can be comparable to the forcing exerted by anthropogenic aerosols on warm clouds. This assessment could therefore support climate models with high sensitivity to greenhouse gas forcing, while still allowing the models to fit the overall historical temperature change.

Probing Aircraft Flight Test Hazard Mitigation for the Alternative Fuel Effects on Contrails & Cruise Emissions (ACCESS) Research Team

NASA Technical Reports Server (NTRS)

Kelly, Michael J.

2013-01-01

The Alternative Fuel Effects on Contrails & Cruise Emissions (ACCESS) Project Integration Manager requested in July 2012 that the NASA Engineering and Safety Center (NESC) form a team to independently assess aircraft structural failure hazards associated with the ACCESS experiment and to identify potential flight test hazard mitigations to ensure flight safety. The ACCESS Project Integration Manager subsequently requested that the assessment scope be focused predominantly on structural failure risks to the aircraft empennage raft empennage.

Earth Observations taken during Expedition Four

NASA Image and Video Library

2002-05-15

ISS004-E-11807 (15 May 2002) --- This digital photograph, taken through the windows of the International Space Station on May 15, 2002, shows condensation trails over the Rhône Valley in the region west of Lyon, France. Condensation trails-or contrails-are straight lines of ice crystals that form in the wake of jet liners where air temperatures are lower than about -40 degrees Centigrade. Scientists have observed that newer contrails are thin whereas older trails have widened with time as a result of light winds. Because of this tendency for thin contrails to cover greater areas with time, it is estimated that these “artificial clouds” cover 0.1 per cent of the planet’s surface. Percentages are far higher in some places, say the scientists, such as southern California, the Ohio River Valley and parts of Europe, as illustrated here. The climatic impact of such clouds is poorly understood, which is why scientists continue to study them using images such as this.

Validation of GOSAT/TANSO-FTS TIR V01.00 CO2 and CH4 products

NASA Astrophysics Data System (ADS)

Saitoh, N.; Kimoto, S.; Sugimura, R.; Imasu, R.; Kawakami, S.; Shiomi, K.; Machida, T.; Sawa, Y.; Matsuda, H.

2014-12-01

Greenhouse Gases Observing Satellite (GOSAT) has been making observations continuously for more than five years since its launch on 23 January 2009. Thermal and Near-infrared Sensor for Carbon Observation Fourier Transform Spectrometer (TANSO-FTS) on board the GOSAT simultaneously observes column abundances and profiles of CO2 and CH4 in the same field of view, from the shortwave infrared (SWIR) and thermal infrared (TIR) bands, respectively. We have just released the latest TIR CO2 and CH4 products, V01.00, to registered researchers. To validate the data quality of the V01.00 TIR CO2 product, we compared the TIR data with CO2 data obtained by Continuous CO2 Measuring Equipment (CME) on board JAL aircraft in Comprehensive Observation Network for Trace gases by Airliner (CONTRAIL) project. The aircraft CO2 data obtained during the level flights were compared with the V01.00 TIR upper tropospheric CO2 data. The CONTRAIL CO2 "profile" data obtained during the ascending and descending flights over several airports were compared with the TIR CO2 profiles. In the profile comparisons, we applied the TIR averaging kernel functions to the coincident CONTRAIL CO2 profiles. The V01.00 upper atmospheric CO2 data agreed to the CONTRAIL level flight CO2 data on average within 0.5-1%. Some TIR CO2 data showed relatively large differences from the nearest aircraft data, which suggests the existence of several problems such as L1B spectral calibration and nighttime cloud detection issues. The TIR V01.00 CO2 profile data from 9 to 13 km showed better agreement to CONTRAIL CO2 data than the a priori. However, the TIR CO2 data at around and below 5 km had low bias of 1-1.5%. The TIR V01.00 CH4 data generally showed reasonable latitudinal distributions as the previous version. In the Antarctic, unexpected high amounts of CH4 were seen in upper troposphere and lower stratosphere in autumn. This is probably because of the problem of simultaneously retrieved ozone concentration.

Imposing strong constraints on tropical terrestrial CO2 fluxes using passenger aircraft based measurements

NASA Astrophysics Data System (ADS)

Niwa, Yosuke; Machida, Toshinobu; Sawa, Yousuke; Matsueda, Hidekazu; Schuck, Tanja J.; Brenninkmeijer, Carl A. M.; Imasu, Ryoichi; Satoh, Masaki

2012-06-01

Because very few measurements of atmospheric carbon dioxide (CO2) are available in the tropics, estimates of surface CO2 fluxes in tropical regions are beset with considerable uncertainties. To improve estimates of tropical terrestrial fluxes, atmospheric CO2 inversion was performed using passenger aircraft based measurements of the Comprehensive Observation Network for Trace gases by Airliner (CONTRAIL) project in addition to the surface measurement data set of GLOBALVIEW-CO2. Regional monthly fluxes at the earth's surface were estimated using the Bayesian synthesis approach focusing on the period 2006-2008 using the Nonhydrostatic Icosahedral Atmospheric Model-based Transport Model (NICAM-TM). By adding the aircraft to the surface data, the posterior flux errors were greatly reduced; specifically, error reductions of up to 64% were found for tropical Asia regions. This strong impact is closely related to efficient vertical transport in the tropics. The optimized surface fluxes using the CONTRAIL data were evaluated by comparing the simulated atmospheric CO2 distributions with independent aircraft measurements of the Civil Aircraft for the Regular Investigation of the atmosphere Based on an Instrument Container (CARIBIC) project. The inversion with the CONTRAIL data yields the global carbon sequestration rates of 2.22 ± 0.28 Pg C yr-1 for the terrestrial biosphere and 2.24 ± 0.27 Pg C yr-1 for the oceans (the both are adjusted by riverine input of CO2). For the first time the CONTRAIL CO2 measurements were used in an inversion system to identify the areas of greatest impact in terms of reducing flux uncertainties.

Case Study Analyses of the Success DC-8 Scanning Lidar Database

NASA Technical Reports Server (NTRS)

Uthe, Edward E.

2000-01-01

Under project SUCCESS (Subsonic Aircraft Contrail and Cloud Effects Special Study) funded by the Atmospheric Effects of Aviation Program, SRI International (SRI) developed an angular scanning back'scatter lidar for operation on the NASA DC-8 research aircraft and deployed the scanning lidar during the SUCCESS field campaign. The primary purpose of the lidar was to generate real-time video displays of clouds and contrails above, ahead of, and below the DC-8 as a means to help position the aircraft for optimum cloud and contrail sampling by onboard in situ sensors, and to help extend the geometrical domain of the in situ sampling records. A large, relatively complex lidar database was collected and several data examples were processed to illustrate the value of the lidar data for interpreting the other data records collected during SUCCESS. These data examples were used to develop a journal publication for the special SUCCESS Geophysical Research Letters issue (reprint presented as Appendix A). The data examples justified data analyses of a larger part of the DC-8 lidar database and is the objective of the current study.

Probing Aircraft Flight Test Hazard Mitigation for the Alternative Fuel Effects on Contrails and Cruise Emissions (ACCESS) Research Team . Volume 2; Appendices

NASA Technical Reports Server (NTRS)

Kelly, Michael J.

2013-01-01

The Alternative Fuel Effects on Contrails and Cruise Emissions (ACCESS) Project Integration Manager requested in July 2012 that the NASA Engineering and Safety Center (NESC) form a team to independently assess aircraft structural failure hazards associated with the ACCESS experiment and to identify potential flight test hazard mitigations to ensure flight safety. The ACCESS Project Integration Manager subsequently requested that the assessment scope be focused predominantly on structural failure risks to the aircraft empennage (horizontal and vertical tail). This report contains the Appendices to Volume I.

The Effects of Commercial Airline Traffic on LSST Observing Efficiency

NASA Astrophysics Data System (ADS)

Gibson, Rose; Claver, Charles; Stubbs, Christopher

2016-01-01

The Large Synoptic Survey Telescope (LSST) is a ten-year survey that will map the southern sky in six different filters 800 times before the end of its run. In this paper, we explore the primary effect of airline traffic on scheduling the LSST observations in addition to the secondary effect of condensation trails, or contrails, created by the presence of the aircraft. The large national investment being made in LSST implies that small improvments observing efficiency through aircraft and contrail avoidance can result in a significant improvement in the quality of the survey and its science. We have used the Automatic Dependent Surveillance-Broadcast (ADS-B) signals received from commercial aircraft to monitor and record activity over the LSST site. We installed a ADS-B ground station on Cerro Pachón, Chile consiting of a1090Mhz antenna on the Andes Lidar Observatory feeding a RTL2832U software defined radio. We used dump1090 to convert the received ADS-B telementry into Basestation format, where we found that during the busiest time of the night there were only 4 signals being received each minute on average, which will have very small direct effect, if any, on the LSST observing scheduler. As part of future studies we will examin the effects of contrals on LSST observations. Gibson was supported by the NOAO/KPNO Research Experiences for Undergraduates (REU) Program which is funded by the National Science Foundation Research Experience for Undergraduates Program (AST-1262829).

Vertical dispersion of an aircraft wake: Aerosol-lidar analysis of entrainment and detrainment in the vortex regime

NASA Astrophysics Data System (ADS)

Sussmann, Ralf

1999-01-01

Vertical dispersion of contrails in the vortex regime is investigated by focusing on the role of entrainment and detrainment of exhaust with respect to the pair of trailing vortices. A ground-based backscatter-depolarization lidar with an integrated CCD camera provides information on optical and geometrical parameters of the contrail in the time span between 5.7 and 50.3 s behind a B747-400 aircraft. This is combined with coincident airborne in situ measurements of turbulence and the vertical profiles of temperature and wind speed in a case study. The two wingtip vortices, separated by 47 m, are descending with an increasing speed (2.5-3.1 m/s for 10.8-47.8 s behind aircraft) in the weakly non-stably-stratified atmosphere. The turbulent vertical dissipation rate on the day of the study above southern Germany is a factor of 1000 higher than found typically above oceans at cruising altitude. At 4.2 s behind the aircraft, a diffuse secondary wake starts to evolve above the two wingtip vortices. After ≈ 50 s the secondary wake encloses a cross-sectional area (4410 m2) comparable to that of the primary wake (4620 m2) and a relative ice surface area of 1:5. The observed early onset of the secondary wake is conjectured to be due to turbulent detrainment of fluid out of the primary wake which can be enhanced by detrainment due to baroclinic forces later in the vortex regime evolution. By exclusion of other mechanisms of secondary wake formation, detrainment of fluid from the primary wake is concluded to be the precondition for secondary wake formation. Detrainment due to baroclinic forces, shear or turbulence is, in general, unlikely to be absent for typical atmospheric conditions. It is suggested that the ambient humidity level may determine when a secondary wake is visible above a vortex pair and when it is not.

The recent changes and topics in CONTRAIL project

NASA Astrophysics Data System (ADS)

Machida, Toshinobu; Umezawa, Taku; Sawa, Yousuke; Niwa, Yosuke; Matsueda, Hidekazu

2016-04-01

CONTRAIL (Comprehensive Observation Network for TRace gases by AIrLiner) project has been conducted since 2005 with Continuous CO2 Measuring Equipment (CME) and Automatic air Sampling Equipment (ASE) onboard commercial airliners for observations of atmospheric greenhouse gases. Japan Airlines (JAL) offers eight Boeing 777-200ER airplanes modified for CONTRAIL; CME and ASE can be installed on all and five of them, respectively. ASE measurements have provided the long records of greenhouse gases in the upper troposphere along the flight route between Japan and Australia. In addition, we started a new sampling program between Japan and Europe in 2012 to obtain data at higher latitudes and the UT/LS region. When the 777-200ER airplane was not operated for the observation route, we used Manual air Sampling Equipment (MSE) for taking air onboard as substitute for ASE. Since flight routes with 777-200ER have been restricted only to Asian countries and Hawaii in the last few years, additional two Boeing 777-300ERs were modified to install CME and expand area coverage of CO2 observations to Europe, Australia and the east coast of US in 2015-2016. We also present our recent data analysis for intensive CME observations over Delhi, India, which indicates a significant impact of Indian wintertime agriculture on the regional carbon budget.

Study Confirms Biofuels Reduce Jet Engine Pollution on This Week @NASA – March 17, 2017

NASA Image and Video Library

2017-03-17

Findings published March 15 in the journal Nature from a series of flight tests in 2013 and 2014 near NASA’s Armstrong Flight Research Center in California indicate that using biofuels helps jet engines reduce particle emissions in exhaust by as much as 50 to 70 percent. That’s both an economic and an environmental benefit. The findings were based on data from the Alternative Fuel Effects on Contrails and Cruise Emissions Study, or ACCESS. The international research program led by NASA and involving agencies from Germany and Canada, studied the effects of alternative fuels on aircraft-generated contrails, engine performance and emissions. Also, NASA @SXSW Interactive Festival, Satellites See Winter Storm from Space, CST-100 Starliner Parachute Testing, and NASA’s Pi Day Challenge!

Modeling the Environmental Impact of Air Traffic Operations

NASA Technical Reports Server (NTRS)

Chen, Neil

2011-01-01

There is increased interest to understand and mitigate the impacts of air traffic on the climate, since greenhouse gases, nitrogen oxides, and contrails generated by air traffic can have adverse impacts on the climate. The models described in this presentation are useful for quantifying these impacts and for studying alternative environmentally aware operational concepts. These models have been developed by leveraging and building upon existing simulation and optimization techniques developed for the design of efficient traffic flow management strategies. Specific enhancements to the existing simulation and optimization techniques include new models that simulate aircraft fuel flow, emissions and contrails. To ensure that these new models are beneficial to the larger climate research community, the outputs of these new models are compatible with existing global climate modeling tools like the FAA's Aviation Environmental Design Tool.

Modeling Macro- and Micro-Scale Turbulent Mixing and Chemistry in Engine Exhaust Plumes

NASA Technical Reports Server (NTRS)

Menon, Suresh

1998-01-01

Simulation of turbulent mixing and chemical processes in the near-field plume and plume-vortex regimes has been successfully carried out recently using a reduced gas phase kinetics mechanism which substantially decreased the computational cost. A detailed mechanism including gas phase HOx, NOx, and SOx chemistry between the aircraft exhaust and the ambient air in near-field aircraft plumes is compiled. A reduced mechanism capturing the major chemical pathways is developed. Predictions by the reduced mechanism are found to be in good agreement with those by the detailed mechanism. With the reduced chemistry, the computer CPU time is saved by a factor of more than 3.5 for the near-field plume modeling. Distributions of major chemical species are obtained and analyzed. The computed sensitivities of major species with respect to reaction step are deduced for identification of the dominant gas phase kinetic reaction pathways in the jet plume. Both the near field plume and the plume-vortex regimes were investigated using advanced mixing models. In the near field, a stand-alone mixing model was used to investigate the impact of turbulent mixing on the micro- and macro-scale mixing processes using a reduced reaction kinetics model. The plume-vortex regime was simulated using a large-eddy simulation model. Vortex plume behind Boeing 737 and 747 aircraft was simulated along with relevant kinetics. Many features of the computed flow field show reasonable agreement with data. The entrainment of the engine plumes into the wing tip vortices and also the partial detrainment of the plume were numerically captured. The impact of fluid mechanics on the chemical processes was also studied. Results show that there are significant differences between spatial and temporal simulations especially in the predicted SO3 concentrations. This has important implications for the prediction of sulfuric acid aerosols in the wake and may partly explain the discrepancy between past numerical studies (that employed parabolic or temporal approximations) and the measured data. Finally to address the major uncertainty in the near-field plume modeling related to the plume processing of sulfur compounds and advanced model was developed to evaluate its impact on the chemical processes in the near wake. A comprehensive aerosol model is developed and it is coupled with chemical kinetics and the axisymmetric turbulent jet flow models. The integrated model is used to simulate microphysical processes in the near-field jet plume, including sulfuric acid and water binary homogeneous nucleation, coagulation, non-equilibrium heteromolecular condensation, and sulfur-induced soot activation. The formation and evolution of aerosols are computed and analyzed. The computed results show that a large number of ultra-fine (0.3--0.6 nm in radius) volatile HSO4 - HO embryos are generated in the near-field plume. These embryos further grow in size by self coagulation and condensation. Soot particles can be activated by both heterogeneous nucleation and scavenging of H2SO4-H2O aerosols. These activated soot particles can serve as water condensation nuclei for contrail formation. Conditions under which ice contrails can form behind aircrafts are studied. The sensitivities of the threshold temperature for contrail formation with respect to aircraft propulsion efficiency, relative humidity, and ambient pressure are evaluated. The computed aerosol properties for different extent of fuel sulfur conversion to S(VI) (SO3 and H2SO4) in engine are examined and the results are found to be sensitive to this conversion fraction.

Contrails over Lisbon, Portugal

NASA Image and Video Library

2008-09-05

This image from NASA EarthKAM shows Lisbon, the capital of Portugal. The superb natural harbor at Lisbon is a commercially important European port handling much of the import-export traffic for Portugal and Spain.

The Effects of Water Vapor and Clouds on the Spectral Distribution of Solar Radiation at the...

NASA Technical Reports Server (NTRS)

Pilewskie, P.; Bergstrom, R.; Mariani, P.; Gore, Warren J. Y. (Technical Monitor)

1997-01-01

During the Subsonic Contrail and Cloud Effect Special Study (SUCCESS) a Solar Spectral Flux Radiometer was deployed at the surface in a zenith observing position. The instrument measured the solar spectral downwelling irradiance between 350 and 2500 nm with 10 nm resolution. From April 12 through April 29 approximately 18000 spectra were acquired, under a variety of meteorological conditions including cloud free, cirrus, Stearns, and cumulonimbus clouds. This study focuses on the effect of cirrus and cirrus contrails on the spectral distribution of solar irradiance at the surface and on inferring cirrus properties from their spectral transmittance. The observations have also proven to be useful for comparing the solar spectral irradiance measurements with model predictions, and in particular, for inferring the amount of solar radiation absorbed in the clear and cloudy atmosphere.

Sulfur Oxidation and Contrail Precursor Chemistry

NASA Technical Reports Server (NTRS)

DeWitt, Kenneth; Hwang, Soon M.

2005-01-01

Sulfuric acid (H2SO4), formed in commercial aircraft operations via Fuel-S (right arrow) SO2 + SO3 (right arrow) H2SO4, plays an important role in affecting the global climate change through atmospheric chemical reactions and radiative forcing. Measurement of the sulfur oxidation rates is critical to the understanding of the contrail formation. The principle reaction pathway is SO2 + O + M (right arrow) SO3 + M. Although there are many measurements for the rates of this reaction, it has never been measured in the temperature and pressure regime available to aircraft operation. In this investigation, a series of experiments were performed behind the reflected shock waves in a shock tube. OH radicals were produced in lean, shock heated SO2/H2/O2/Ar mixtures. The reaction progress was followed using OH absorption spectroscopy at 310 nm. The data were analyzed with the aid of computer modeling/simulation. The mean value of the rate coefficients of R21 determined is k(sub 21,0)/[M]= 3.9 x 10(exp 15) cm(sup 6) per square mole per second at T = 960 - 1150 K and rho = 16-30 micromole per cubic centimeter with uncertainty limits of plus or minus 30%. A non-Arrhenius fit to our data together with all existing data gives k(sub 21,0)/[M] = 1.3 x 10(exp 24) T (exp -2.5) exp(-2350 K/T) cm(sup 6) per square mole per second at T = 300 - 2500 K with the same uncertainty limits given above. The calculated conversion of S(IV)(SO2) to S(VI) (SO3 + H2SO4) was about 2% in our experimental conditions.

Identification of Flights for Cost-Efficient Climate Impact Reduction

NASA Technical Reports Server (NTRS)

Chen, Neil Y.; Kirschen, Philippe G.; Sridhar, Banavar; Ng, Hok K.

2014-01-01

The aircraft-induced climate impact has drawn attention in recent years. Aviation operations affect the environment mainly through the release of carbon-dioxide, nitrogen-oxides, and by the formation of contrails. Recent research has shown that altering trajectories can reduce aviation environmental cost by reducing Absolute Global Temperature Change Potential, a climate assessment metric that adapts a linear system for modeling the global temperature response to aviation emissions and contrails. However, these methods will increase fuel consumption that leads to higher fuel costs imposed on airlines. The goal of this work is to identify ights for which the environmental cost of climate impact reduction outweighs the increase in operational cost on an individual aircraft basis. Environmental cost is quanti ed using the monetary social cost of carbon. The increase in operational cost is considering cost of additional fuel usage only. For this paper, an algorithm has been developed that modi es the trajectories of ights to evaluate the e ect of environ- mental cost and operational cost of ights in the United States National Airspace System. The algorithm identi es ights for which the environmental cost of climate impact can be reduced and modi es their trajectories to achieve maximum environmental net bene t, which is the di erence between reduction in environmental cost and additional operational cost. The result shows on a selected day, 16% of the ights among eight major airlines, or 2,043 ights, can achieve environmental net bene t using weather forecast data, resulting in net bene t of around $500,000. The result also suggests that the long-haul ights would be better candidates for cost-ecient climate impact reduction than the short haul ights. The algorithm will help to identify the characteristics of ights that are capable of applying cost-ecient climate impact reduction strategy.

Surface CO2 Flux in Weekly Time Resolution Over the Globe Inferred From CONTRAIL Data set

NASA Astrophysics Data System (ADS)

Taguchi, S.; Machida, T.; Matsueda, H.; Sawa, Y.

2008-12-01

Concentrations of CO2 observed on passenger aircrafts are ready for data assimilation in biogeochemical models. Five auto measurement system called the continuous CO2 measuring Equipments (CME) are installed on Boeing 747 and 777 and are measuring CO2 in every 10 second in ascending and descending mode and every 1 minute during level flight (Machida et al., doi:10.1175/2008JTECHA1082.1). The measurement system, named comprehensive observation network for trace gases by airliner (CONTRAIL) has been tested in 2006 and is in full operation since November 2006. In this presentation, we will show a preliminary result of inverse calculation to estimate weekly sources and sinks of CO2 in 2007 at 64 surface areas on the globe. About 30000 data world wide extending from 3km to 11 km in 2007 were selected from full data set due to a limitation of our solver. A global atmospheric transport model driven with a meteorological data set of ECMWF was used to derive a gain matrix which represents a response at a sampling point of concentrations from a continuous release of CO2 for a week at individual area. Fluxes in 56 weeks starting from 5th December 2006 were estimated. The root mean squared error between concentrations simulated using weekly fluxes and CONTRAIL was 1.6ppm which improved 12 percent from that of concentrations simulated using monthly fluxes estimated from other data set.

Wildfires in Eastern U.S.

NASA Technical Reports Server (NTRS)

2002-01-01

Drought conditions have plagued the Appalachian Mountains in October and November, and low relative humidity combined with dry leaves on the ground has created extreme fire danger in many eastern states. This true-color MODIS image made from data collected on November 13, 2001, shows smoke from numerous fires (indicated in red), predominantly in southern West Virginia (image center), Kentucky (to the southwest), and Tennessee (south). The fires, at least some of which are likely the result of arson, have burned thousands of acres throughout the region. Unfortunately for those people fighting the fires, the fire danger is likely to remain high, with no significant rain expected in the near term. South of Lake Erie, the southernmost of the Great Lakes, numerous aircraft contrails crisscross Ohio. Water vapor emitted with engine exhaust condenses in the cold, dry air at high altitudes, leaving behind a trail of condensation--a contrail. Image courtesy Jacques Descloitres, MODIS Land Rapid Response Team at NASA GSFC

Retrievals of Aerosol and Cloud Particle Microphysics Using Polarization and Depolarization Techniques

NASA Technical Reports Server (NTRS)

Mishchenko, Michael; Hansen, James E. (Technical Monitor)

2001-01-01

The recent availability of theoretical techniques for computing single and multiple scattering of light by realistic polydispersions of spherical and nonspherical particles and the strong dependence of the Stokes scattering matrix on particle size, shape, and refractive index make polarization and depolarization measurements a powerful particle characterization tool. In this presentation I will describe recent applications of photopolarimetric and lidar depolarization measurements to remote sensing characterization of tropospheric aerosols, polar stratospheric clouds (PSCs), and contrails. The talk will include (1) a short theoretical overview of the effects of particle microphysics on particle single-scattering characteristics; (2) the use of multi-angle multi-spectral photopolarimetry to retrieve the optical thickness, size distribution, refractive index, and number concentration of tropospheric aerosols over the ocean surface; and (3) the application of the T-matrix method to constraining the PSC and contrail particle microphysics using multi-spectral measurements of lidar backscatter and depolarization.

Winter crop CO2 uptake inferred from CONTRAIL CO2 measurements over Delhi, India

NASA Astrophysics Data System (ADS)

Umezawa, T.; Niwa, Y.; Sawa, Y.; Machida, T.; Matsueda, H.

2016-12-01

CONTRAIL is an ongoing project that measures atmospheric trace gases onboard aircraft of Japan Airlines. Atmospheric CO2 concentration is analyzed using Continuous CO2 Measuring Equipment (CME) during intercontinental flights. Since 2005, we have obtained >7 millions of data points of CO2 concentration along level-flight and ascent/descent tracks of >12 thousands flights with extensive coverage of the Asia-Pacific region. In this study, we analyze 787 vertical profiles of CO2 over Delhi, India. The surrounding area is mainly covered by irrigated croplands with patchy urban areas. We observed a general increase of CO2 toward the ground in the boundary layer throughout December-April due to urban CO2 emissions from the Delhi metropolitan area. In January-March, however, we frequently observed sharp decreases of CO2 below 2 km, indicating the existence of local CO2 sinks in this season. We calculated enhancement/depletion of CO2 amount in the boundary layer, and found clear depletion in February-March, coincident with the growing season of the winter crops (mainly wheat) in the region. It is also inferred that the crop uptake may exceed in magnitude the urban anthropogenic emissions from the Delhi area, indicating significance of agricultural CO2 fluxes in the regional carbon budget. Due to the winter crop uptake, CO2 concentration over Delhi shows no increasing/decreasing temporal trends during January-March when that at baseline stations at similar latitudes in the northern hemisphere increases steadily. This suggests that the CONTRAIL measurements capture local to regional flux signals that are not well resolved by the existing observation network.

Landing of STS-63 Discovery at KSC

NASA Technical Reports Server (NTRS)

1995-01-01

Contrails stream from the port side wing of the Space Shuttle Discovery as it touches down on Runway 15 at the Kennedy Space Center's (KSC) Shuttle Landing Facility to complete an eight day mission. Touchdown occurred at 6:50:19 a.m. (EST), February 11, 1995.

Integration of Linear Dynamic Emission and Climate Models with Air Traffic Simulations

NASA Technical Reports Server (NTRS)

Sridhar, Banavar; Ng, Hok K.; Chen, Neil Y.

2012-01-01

Future air traffic management systems are required to balance the conflicting objectives of maximizing safety and efficiency of traffic flows while minimizing the climate impact of aviation emissions and contrails. Integrating emission and climate models together with air traffic simulations improve the understanding of the complex interaction between the physical climate system, carbon and other greenhouse gas emissions and aviation activity. This paper integrates a national-level air traffic simulation and optimization capability with simple climate models and carbon cycle models, and climate metrics to assess the impact of aviation on climate. The capability can be used to make trade-offs between extra fuel cost and reduction in global surface temperature change. The parameters in the simulation can be used to evaluate the effect of various uncertainties in emission models and contrails and the impact of different decision horizons. Alternatively, the optimization results from the simulation can be used as inputs to other tools that monetize global climate impacts like the FAA s Aviation Environmental Portfolio Management Tool for Impacts.

Winter crop CO2 uptake inferred from CONTRAIL measurements over Delhi, India

NASA Astrophysics Data System (ADS)

Umezawa, Taku; Niwa, Yosuke; Sawa, Yousuke; Machida, Toshinobu; Matsueda, Hidekazu

2016-11-01

Recent studies have shown the impact of expanding agricultural activities on atmospheric CO2 variations and the global carbon cycle. In this study, we show clear evidence of the measureable impact of Indian wintertime crops (mainly wheat) on the regional carbon budget using high-frequency atmospheric CO2 measurements by Comprehensive Observation Network for Trace gases by Airliners (CONTRAIL) over Delhi; this phenomenon is not detected by the existing network of surface CO2 sites. While a general increase in the vertical profiles of CO2 toward the ground in the boundary layer was observed throughout December-April, we frequently observed sharp decreases below 2 km during January-March. Seasonal circulations during these 3 months indicated influences from neighboring croplands (with patchy urban areas) located upwind. We conclude that the observed CO2 decrease is attributable to active uptake by the crops grown in winter and that the uptake exceeds in magnitude the urban CO2 emissions from the Delhi metropolitan area.

Probabilistic description of ice-supersaturated layers in low resolution profiles of relative humidity

NASA Astrophysics Data System (ADS)

Dickson, N. C.; Gierens, K. M.; Rogers, H. L.; Jones, R. L.

2010-07-01

The global observation, assimilation and prediction in numerical models of ice super-saturated (ISS) regions (ISSR) are crucial if the climate impact of aircraft condensation trails (contrails) is to be fully understood, and if, for example, contrail formation is to be avoided through aircraft operational measures. Given their small scales compared to typical atmospheric model grid sizes, statistical representations of the spatial scales of ISSR are required, in both horizontal and vertical dimensions, if global occurrence of ISSR is to be adequately represented in climate models. This paper uses radiosonde launches made by the UK Meteorological Office, from the British Isles, Gibraltar, St. Helena and the Falkland Islands between January 2002 and December 2006, to investigate the probabilistic occurrence of ISSR. Each radiosonde profile is divided into 50- and 100-hPa pressure layers, to emulate the coarse vertical resolution of some atmospheric models. Then the high resolution observations contained within each thick pressure layer are used to calculate an average relative humidity and an ISS fraction for each individual thick pressure layer. These relative humidity pressure layer descriptions are then linked through a probability function to produce an s-shaped curve which empirically describes the ISS fraction in any average relative humidity pressure layer. Using this empirical understanding of the s-shaped relationship a mathematical model was developed to represent the ISS fraction within any arbitrary thick pressure layer. Two models were developed to represent both 50- and 100-hPa pressure layers with each reconstructing their respective s-shapes within 8-10% of the empirical curves. These new models can be used, to represent the small scale structures of ISS events, in modelled data where only low vertical resolution is available. This will be useful in understanding, and improving the global distribution, both observed and forecasted, of ice super-saturation.

Persian Gulf Contrail Altitude Limits

DTIC Science & Technology

1991-06-01

71000 APR 5500 32800 50200 68500 MAT 25000 34800 50600 73500 JUN 31500 43400 57400 84500 JUL 39500 45200 50800 70500 AUG 35000 44200 58800 81500 SEP 33500...47900 65000 HAT 30500 38200 49900 61000 Jui3~4500 44200 54600 69500 JUL 18500 45100 51600 67000 AUG3 39000 M4300 54500 69500 SEP 34500 43500 53900

Nutrient transitions are a source of persisters in Escherichia coli biofilms.

PubMed

Amato, Stephanie M; Brynildsen, Mark P

2014-01-01

Chronic and recurrent infections have been attributed to persisters in biofilms, and despite this importance, the mechanisms of persister formation in biofilms remain unclear. The plethora of biofilm characteristics that could give rise to persisters, including slower growth, quorum signaling, oxidative stress, and nutrient heterogeneity, have complicated efforts to delineate formation pathways that generate persisters during biofilm development. Here we sought to specifically determine whether nutrient transitions, which are a common metabolic stress encountered within surface-attached communities, stimulate persister formation in biofilms and if so, to then identify the pathway. To accomplish this, we established an experimental methodology where nutrient availability to biofilm cells could be controlled exogenously, and then used that method to discover that diauxic carbon source transitions stimulated persister formation in Escherichia coli biofilms. Previously, we found that carbon source transitions stimulate persister formation in planktonic E. coli cultures, through a pathway that involved ppGpp and nucleoid-associated proteins, and therefore, tested the functionality of that pathway in biofilms. Biofilm persister formation was also found to be dependent on ppGpp and nucleoid-associated proteins, but the importance of specific proteins and enzymes between biofilm and planktonic lifestyles was significantly different. Data presented here support the increasingly appreciated role of ppGpp as a central mediator of bacterial persistence and demonstrate that nutrient transitions can be a source of persisters in biofilms.

Genetics of Persister Formation in Pseudomonas aeruginosa

DTIC Science & Technology

2012-12-14

RNA endonuclease toxin-anti-toxin modules must be knocked out before there is an observable effect on persister formation (Maisonneuve, Shakespeare et...multidrug tolerance in Escherichia coli." J Bacteriol 186(24): 8172-8180. Maisonneuve, E., L. J. Shakespeare , et al. (2011). "Bacterial persistence by RNA...endonuclease toxin-anti-toxin modules must be knocked out before there is an observable effect on persister formation (Maisonneuve, Shakespeare et al. 2011

Inter-annual variations of CO2 observed by commercial airliner in the CONTRAIL project

NASA Astrophysics Data System (ADS)

Sawa, Yousuke; Machida, Toshinobu; Matsueda, Hidekazu; Niwa, Yosuke; Umezawa, Taku

2016-04-01

Since 2005, we have conducted an observation program for greenhouse gases using the passenger aircraft of the Japan Airlines named Comprehensive Observation Network for TRace gases by AIrLiner (CONTRAIL). Over the past 10 years, successful operation of Continuous CO2 Measuring Equipment (CME) has delivered more than 6 million in-situ CO2 data from about 12000 flights between Japan and Europe, Australia, North America, or Asia. The large number of CME data enable us to well characterize spatial distributions and seasonal changes of CO2 in wide regions of the globe especially the Asia-Pacific regions. While the mean growth rates for the past 10 years were about 2 ppm/year, large growth rates of about 3 ppm/year were found in the wide latitudinal bands from 30S to 70N from the second half of 2012 to the first half of 2013. The multiyear data sets have the potential to help understand the global/regional CO2 budget. One good example is the significant inter-annual difference in CO2 vertical profiles observed over Singapore between October 2014 and October 2015, which is attributable to the massive biomass burnings in Indonesia in 2015.

Survival of bactericidal antibiotic treatment by tolerant persister cells of Klebsiella pneumoniae.

PubMed

Li, Ying; Zhang, Luhua; Zhou, Yingshun; Zhang, Zhikun; Zhang, Xinzhuo

2018-03-01

Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infections. In this study, we aimed to investigate the persister subpopulation of K. pneumoniae. The presence of persisters in K. pneumoniae was determined by treatment with high concentrations of antibiotics, used alone or in combination. The effect of low level of antibiotics on persister formation was investigated by pre-exposure of cells to antibiotics with low concentrations followed by higher doses. The dependence of persister levels on growth phase was determined by measuring the survival ability of cells along the growth stages upon exposure to a high concentration of antibiotic. Analysis on persister type was carried out by persister elimination assays.Results/Key findings. We show that K. pneumoniae produces high levels of tolerant persister cells to survive treatment by a variety of high concentrations of bactericidal antibiotics and persister formation is prevalent among K. pneumoniae clinical strains. Besides, we find that persister cells can be induced by low concentrations of antibiotics. Finally, we provide evidence that persister formation is growth phase-dependent and Type II persisters dominate the persister subpopulation during the entire exponential phase of K. pneumoniae. Our study describes the formation of tolerant persister cells that allow survival of treatment by high concentrations of antibiotics in K. pneumoniae.

The defective nature of ice Ic and its implications for atmospheric science

NASA Astrophysics Data System (ADS)

Kuhs, W. F.; Hansen, T. C.

2009-04-01

The possible atmospheric implication of ice Ic (cubic ice) has already been suggested some time ago in the context of snow crystal formation [1]. New findings from air-borne measurements in cirrus clouds and contrails have put ice Ic into the focus of interest to understand the so-called "supersaturation puzzle" [2,3,4,5]. Our recent microstructural work on ice Ic [6,7] appears to be highly relevant in this context. We have found that ice Ic is characterized by a complex stacking fault pattern, which changes as a function of temperature as well as time. Indeed, from our own [8] and other group's work [9] one knows that (in contrast to earlier believe) ice Ic can form up to temperatures at least as high as 240K - thus in the relevant range for cirrus clouds. We have good preliminary evidence that the "cubicity" (which can be related to stacking fault probabilities) as well as the particle size of ice Ic are the relevant parameters for this correlation. The "cubicity" of stacking faulty ice Ic (established by diffraction) correlates nicely with the increased supersaturation at decreasing temperatures observed in cirrus clouds and contrails, a fact, which may be considered as further evidence for the presence of ice Ic. Moreover, the stacking faults lead to kinks in the outer shapes of the minute ice Ic crystals as seen by cryo scanning electron microscopy (cryo-SEM); these defective sites are likely to play some role in heterogeneous reactions in the atmosphere. The cryo-SEM work suggests that stacking-faulty ice Ic has many more active centres for such reactions than the usually considered thermodynamically stable form, ice Ih. [1] T Kobayashi & T Kuroda (1987) Snow Crystals. In: Morphology of Crystals (ed. I Sunagawa), Terra Scientific Publishing, Tokyo, pp.649-743. [2] DM Murphy (2003) Dehydration in cold clouds is enhanced by a transition from from cubic to hexagonal ice. Geophys.Res.Lett.,30, 2230, doi:10.1029/2003GL018566. [3] RS Gao & 19 other authors (2004) Evidence that nitric acid increases relative humidity in low-temperature cirrus clouds. Science 303, 516-520. [4] T Peter, C Marcolli, P Spaichinger, T Corti, MC Baker & T Koop (2006) When dry air is too humid. Science 314, 1399-1402. [5] JE Shilling, MA Tolbert, OB Toon, EJ Jensen, BJ Murray & AK Bertram (2006) Measurements of the vapor pressure of cubic ice and their implications for atmospheric ice clouds. Geophys.Res.Lett. 33, 026671. [6] TC Hansen, MM Koza & WF Kuhs (2008) Formation and annealing of cubic ice: I Modelling of stacking faults. J.Phys.Cond.Matt. 20, 285104. [7] TC Hansen, MM Koza, P Lindner & WF Kuhs (2008) Formation and annealing of cubic ice: II. Kinetic study. J.Phys.Cond.Matt. 20, 285105. [8] WF Kuhs, G Genov, DK Staykova & AN Salamatin (2004) Ice perfection and the onset of anomalous preservation of gas hydrates. Phys.Chem.Chem.Phys. 6, 4917-4920. [9] BJ Murray, DA Knopf & AK Bertram (2005) The formation of cubic ice under conditions relevant to Earth's atmosphere. Nature 434, 292-205.

The differential role of cortical protein synthesis in taste memory formation and persistence

NASA Astrophysics Data System (ADS)

Levitan, David; Gal-Ben-Ari, Shunit; Heise, Christopher; Rosenberg, Tali; Elkobi, Alina; Inberg, Sharon; Sala, Carlo; Rosenblum, Kobi

2016-05-01

The current dogma suggests that the formation of long-term memory (LTM) is dependent on protein synthesis but persistence of the memory trace is not. However, many of the studies examining the effect of protein synthesis inhibitors (PSIs) on LTM persistence were performed in the hippocampus, which is known to have a time-dependent role in memory storage, rather than the cortex, which is considered to be the main structure to store long-term memories. Here we studied the effect of PSIs on LTM formation and persistence in male Wistar Hola (n⩾5) rats by infusing the protein synthesis inhibitor, anisomycin (100 μg, 1 μl), into the gustatory cortex (GC) during LTM formation and persistence in conditioned taste aversion (CTA). We found that local anisomycin infusion to the GC before memory acquisition impaired LTM formation (P=8.9E-5), but had no effect on LTM persistence when infused 3 days post acquisition (P=0.94). However, when we extended the time interval between treatment with anisomycin and testing from 3 days to 14 days, LTM persistence was enhanced (P=0.01). The enhancement was on the background of stable and non-declining memory, and was not recapitulated by another amnesic agent, APV (10 μg, 1 μl), an N-methyl-D-aspartate receptor antagonist (P=0.54). In conclusion, CTA LTM remains sensitive to the action of PSIs in the GC even 3 days following memory acquisition. This sensitivity is differentially expressed between the formation and persistence of LTM, suggesting that increased cortical protein synthesis promotes LTM formation, whereas decreased protein synthesis promotes LTM persistence.

Estimating Asian terrestrial carbon fluxes from CONTRAIL aircraft and surface CO2 observations for the period 2006 to 2010

NASA Astrophysics Data System (ADS)

Zhang, H. F.; Chen, B. Z.; van der Laan-Luijkx, I. T.; Machida, T.; Matsueda, H.; Sawa, Y.; Fukuyama, Y.; Labuschagne, C.; Langenfelds, R.; van der Schoot, M.; Xu, G.; Yan, J. W.; Zhou, L. X.; Tans, P. P.; Peters, W.

2013-10-01

Current estimates of the terrestrial carbon fluxes in Asia ("Asia" refers to lands as far west as the Urals and is divided into Boreal Eurasia, Temperate Eurasia and tropical Asia based on TransCom regions) show large uncertainties particularly in the boreal and mid-latitudes and in China. In this paper, we present an updated carbon flux estimate for Asia by introducing aircraft CO2 measurements from the CONTRAIL (Comprehensive Observation Network for Trace gases by Airline) program into an inversion modeling system based on the CarbonTracker framework. We estimated the averaged annual total Asian terrestrial land CO2 sink was about -1.56 Pg C yr-1 over the period 2006-2010, which offsets about one-third of the fossil fuel emission from Asia (+4.15 Pg C yr-1). The uncertainty of the terrestrial uptake estimate was derived from a set of sensitivity tests and ranged from -1.07 to -1.80 Pg C yr-1, comparable to the formal Gaussian error of ±1.18 Pg C yr-1 (1-sigma). The largest sink was found in forests, predominantly in coniferous forests (-0.64 Pg C yr-1) and mixed forests (-0.14 Pg C yr-1); and the second and third large carbon sinks were found in grass/shrub lands and crop lands, accounting for -0.44 Pg C yr-1 and -0.20 Pg C yr-1, respectively. The peak-to-peak amplitude of inter-annual variability (IAV) was 0.57 Pg C yr-1 ranging from -1.71 Pg C yr-1 to -2.28 Pg C yr-1. The IAV analysis reveals that the Asian CO2 sink was sensitive to climate variations, with the lowest uptake in 2010 concurrent with summer flood/autumn drought and the largest CO2 sink in 2009 owing to favorable temperature and plentiful precipitation conditions. We also found the inclusion of the CONTRAIL data in the inversion modeling system reduced the uncertainty by 11% over the whole Asian region, with a large reduction in the southeast of Boreal Eurasia, southeast of Temperate Eurasia and most Tropical Asian areas.

Imposing strong constraints on tropical terrestrial CO2 fluxes using passenger aircraft based measurements

NASA Astrophysics Data System (ADS)

Niwa, Y.; Machida, T.; Sawa, Y.; Matsueda, H.; Schuck, T. J.; Brenninkmeijer, C. A.; Imasu, R.; Satoh, M.

2011-12-01

Better understanding of the global and regional carbon budget is needed to perform a reliable prediction of future climate with an earth system model. However, the reliability of CO2 source/sink estimation by inverse modeling, which is one of the promising methods to estimate regional carbon budget, is limited because of sparse observational data coverage. Very few observational data are available in tropics. Therefore, especially the reconstruction of tropical terrestrial fluxes has considerable uncertainties. In this study, regional CO2 fluxes for 2006-2008 are estimated by inverse modeling using the Comprehensive Observation Network for Trace gases by Airliner (CONTRAIL) in addition to the surface measurement dataset of GLOBALVIEW-CO2. CONTRAIL is a recently established CO2 measurement network using in-situ measurement instruments on board commercial aircraft. Five CONTRAIL aircraft travel back and forth between Japan and many areas: Europe, North America, Southeast Asia, South Asia, and Australia. The Bayesian synthesis approach is used to estimate monthly fluxes for 42 regions using NICAM-TM simulations with existing CO2 flux datasets and monthly mean observational data. It is demonstrated that the aircraft data have great impact on estimated tropical terrestrial fluxes. By adding the aircraft data to the surface data, the analyzed uncertainty of tropical fluxes has been reduced by 15 % and more than 30 % uncertainty reduction rate is found in Southeast and South Asia. Specifically, for annual net CO2 fluxes, nearly neutral fluxes of Indonesia, which is estimated using the surface dataset alone, turn to positive fluxes, i.e. carbon sources. In Indonesia, a remarkable carbon release during the severe drought period of October-December in 2006 is estimated, which suggests that biosphere respiration or biomass burning was larger than the prior fluxes. Comparison of the optimized atmospheric CO2 with independent aircraft measurements of CARIBIC tends to validate results of the inversion system. It is expected that the use of instantaneous observational data with more sophisticated inversion methods will provide more accurate estimation of surface CO2 fluxes.

Characteristics of Four-years of GOSAT/TANSO-FTS TIR V1.0 CO2 and CH4 Products

NASA Astrophysics Data System (ADS)

Saitoh, N.; Kimoto, S.; Sugimura, R.; Imasu, R.; Shiomi, K.; Kuze, A.; Kataoka, F.; Knuteson, R. O.; Machida, T.; Sawa, Y.; Matsuda, H.

2015-12-01

Greenhouse Gases Observing Satellite (GOSAT) was launched on 23 January 2009, and has continued to make global observations, including both nadir and off-nadir measurements, for more than six years since its launch. The thermal infrared (TIR) band of Thermal and Near-infrared Sensor for Carbon Observation Fourier Transform Spectrometer (TANSO-FTS) on board the GOSAT has observed CO2 and CH4 profiles. We have analyzed the four-year data from 2010 through 2013 of the latest released version of the TIR Level 2 (L2) CO2 and CH4 products (V1.0). Comparisons of the TIR upper atmospheric CO2 product with CO2 data from Comprehensive Observation Network for Trace Gases by Airliner (CONTRAIL) aircraft measurements show that the growth rate estimated from the TIR CO2 data is slightly lower than that from the CONTRAIL data. Overall, the TIR V1.0 CO2 product has better quality in the upper troposphere and lower stratosphere than the a priori judging from comparisons with the collocated aircraft data. In spring and summer, however, the quality of the TIR L2 CO2 products became slightly worse than in the other seasons, especially in the low and northern-mid latitudes. This is because the corresponding a priori had a larger bias and the TIR Level 1B (L1B) radiance spectra might have a larger bias in the spring-summer seasons. Here, we have tested several types of correction methods to modify the L1B spectral bias, and then compared CO2 and CH4 concentrations retrieved after applying spectral bias correction factors with coincident CONTRAIL and HIAPER Pole-to-Pole Observation (HIPPO) aircraft data. The comparison results suggest that the L1B spectral bias correction factor should be changed depending on wavelength. In addition, it should be expressed as a function of on-board internal calibration blackbody temperatures. This is because they are weak season-dependent parameters; they were clearly lower in spring and summer.

The MqsRA Toxin-Antitoxin System from Xylella fastidiosa Plays a Key Role in Bacterial Fitness, Pathogenicity, and Persister Cell Formation.

PubMed

Merfa, Marcus V; Niza, Bárbara; Takita, Marco A; De Souza, Alessandra A

2016-01-01

Through the formation of persister cells, bacteria exhibit tolerance to multidrug and other environmental stresses without undergoing genetic changes. The toxin-antitoxin (TA) systems are involved in the formation of persister cells because they are able to induce cell dormancy. Among the TA systems, the MqsRA system has been observed to be highly induced in persister cells of Xylella fastidiosa (causal agent of citrus variegated chlorosis-CVC) activated by copper stress, and has been described in Escherichia coli as related to the formation of persister cells and biofilms. Thus, we evaluated the role of this TA system in X. fastidiosa by overexpressing the MqsR toxin, and verified that the toxin positively regulated biofilm formation and negatively cell movement, resulting in reduced pathogenicity in citrus plants. The overexpression of MqsR also increased the formation of persister cells under copper stress. Analysis of the gene and protein expression showed that this system likely has an autoregulation mechanism to express the toxin and antitoxin in the most beneficial ratio for the cell to oppose stress. Our results suggest that this TA system plays a key role in the adaptation and survival of X. fastidiosa and reveal new insights into the physiology of phytopathogen-host interactions.

The MqsRA Toxin-Antitoxin System from Xylella fastidiosa Plays a Key Role in Bacterial Fitness, Pathogenicity, and Persister Cell Formation

PubMed Central

Merfa, Marcus V.; Niza, Bárbara; Takita, Marco A.; De Souza, Alessandra A.

2016-01-01

Through the formation of persister cells, bacteria exhibit tolerance to multidrug and other environmental stresses without undergoing genetic changes. The toxin-antitoxin (TA) systems are involved in the formation of persister cells because they are able to induce cell dormancy. Among the TA systems, the MqsRA system has been observed to be highly induced in persister cells of Xylella fastidiosa (causal agent of citrus variegated chlorosis—CVC) activated by copper stress, and has been described in Escherichia coli as related to the formation of persister cells and biofilms. Thus, we evaluated the role of this TA system in X. fastidiosa by overexpressing the MqsR toxin, and verified that the toxin positively regulated biofilm formation and negatively cell movement, resulting in reduced pathogenicity in citrus plants. The overexpression of MqsR also increased the formation of persister cells under copper stress. Analysis of the gene and protein expression showed that this system likely has an autoregulation mechanism to express the toxin and antitoxin in the most beneficial ratio for the cell to oppose stress. Our results suggest that this TA system plays a key role in the adaptation and survival of X. fastidiosa and reveal new insights into the physiology of phytopathogen-host interactions. PMID:27375608

SeaWiFS: The Western United States and Mexico

NASA Technical Reports Server (NTRS)

2002-01-01

The linear patterns in the clouds over the Pacific suggest contrail origins. Subtle variations in cloud density reveal vortex street downwind (southeast) of Mexico's Guadalupe Island. The Great Salt Lake in Utah is divided into two very different colored bodies of water by a railroad causeway. The southern Gulf of California continues to bloom brightly. Credit: Provided by the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

Himalayan Foothills, Bangladesh

NASA Technical Reports Server (NTRS)

1992-01-01

This remarkably clear, pre-monsoon view of the Himalayan foothills of Bangladesh (26.0N, 89.5E) shows the deforestation of the lower slopes for agriculture and pasture lands. The cleared lower slopes are generally used for tea cultivation. The intensity of agricultural land use, mostly in the form of small, family subsistance farms on the Ganges Plain is evident over most of the scene. Note also, the aircraft contrail and Tista River.

Private Militias: The Cancer of the American Society

DTIC Science & Technology

2001-04-01

large legal settlements against the traditional hate groups (the Ku Klux Klan and Aryan Nations), membership enrollment declined in the 1990s; whereas...the legal settlements against the “hate groups” have driven many of their members to either seek out other organizations with shared beliefs or to...take away the legal rights of Americans, committed the OKC bombing, spread toxins across America via contrails from aircraft, attempted to cause a

National Plan for Aeronautics Research and Development and Related Infrastructure

DTIC Science & Technology

2007-12-01

change—is not well understood. FUNDAMENTAL ENERGY AND ENVIRONMENTAL CHALLENGES TO OVERCOME Concerns about aviation’s environmental impacts and...relationships between long-term impacts like CO2 and shorter lived impacts like NOx emissions, contrails and cirrus clouds . ENERGY AND ENVIRONMENT R&D GOALS...abling future generations of aircraft (N+1, N+2, and N+3) that permit better management of the energy resources and environmental impact ; and (2

Biofuel blending reduces particle emissions from aircraft engines at cruise conditions

NASA Astrophysics Data System (ADS)

Moore, Richard H.; Thornhill, Kenneth L.; Weinzierl, Bernadett; Sauer, Daniel; D'Ascoli, Eugenio; Kim, Jin; Lichtenstern, Michael; Scheibe, Monika; Beaton, Brian; Beyersdorf, Andreas J.; Barrick, John; Bulzan, Dan; Corr, Chelsea A.; Crosbie, Ewan; Jurkat, Tina; Martin, Robert; Riddick, Dean; Shook, Michael; Slover, Gregory; Voigt, Christiane; White, Robert; Winstead, Edward; Yasky, Richard; Ziemba, Luke D.; Brown, Anthony; Schlager, Hans; Anderson, Bruce E.

2017-03-01

Aviation-related aerosol emissions contribute to the formation of contrail cirrus clouds that can alter upper tropospheric radiation and water budgets, and therefore climate. The magnitude of air-traffic-related aerosol-cloud interactions and the ways in which these interactions might change in the future remain uncertain. Modelling studies of the present and future effects of aviation on climate require detailed information about the number of aerosol particles emitted per kilogram of fuel burned and the microphysical properties of those aerosols that are relevant for cloud formation. However, previous observational data at cruise altitudes are sparse for engines burning conventional fuels, and no data have previously been reported for biofuel use in-flight. Here we report observations from research aircraft that sampled the exhaust of engines onboard a NASA DC-8 aircraft as they burned conventional Jet A fuel and a 50:50 (by volume) blend of Jet A fuel and a biofuel derived from Camelina oil. We show that, compared to using conventional fuels, biofuel blending reduces particle number and mass emissions immediately behind the aircraft by 50 to 70 per cent. Our observations quantify the impact of biofuel blending on aerosol emissions at cruise conditions and provide key microphysical parameters, which will be useful to assess the potential of biofuel use in aviation as a viable strategy to mitigate climate change.

Biofuel blending reduces particle emissions from aircraft engines at cruise conditions.

PubMed

Moore, Richard H; Thornhill, Kenneth L; Weinzierl, Bernadett; Sauer, Daniel; D'Ascoli, Eugenio; Kim, Jin; Lichtenstern, Michael; Scheibe, Monika; Beaton, Brian; Beyersdorf, Andreas J; Barrick, John; Bulzan, Dan; Corr, Chelsea A; Crosbie, Ewan; Jurkat, Tina; Martin, Robert; Riddick, Dean; Shook, Michael; Slover, Gregory; Voigt, Christiane; White, Robert; Winstead, Edward; Yasky, Richard; Ziemba, Luke D; Brown, Anthony; Schlager, Hans; Anderson, Bruce E

2017-03-15

Aviation-related aerosol emissions contribute to the formation of contrail cirrus clouds that can alter upper tropospheric radiation and water budgets, and therefore climate. The magnitude of air-traffic-related aerosol-cloud interactions and the ways in which these interactions might change in the future remain uncertain. Modelling studies of the present and future effects of aviation on climate require detailed information about the number of aerosol particles emitted per kilogram of fuel burned and the microphysical properties of those aerosols that are relevant for cloud formation. However, previous observational data at cruise altitudes are sparse for engines burning conventional fuels, and no data have previously been reported for biofuel use in-flight. Here we report observations from research aircraft that sampled the exhaust of engines onboard a NASA DC-8 aircraft as they burned conventional Jet A fuel and a 50:50 (by volume) blend of Jet A fuel and a biofuel derived from Camelina oil. We show that, compared to using conventional fuels, biofuel blending reduces particle number and mass emissions immediately behind the aircraft by 50 to 70 per cent. Our observations quantify the impact of biofuel blending on aerosol emissions at cruise conditions and provide key microphysical parameters, which will be useful to assess the potential of biofuel use in aviation as a viable strategy to mitigate climate change.

Workshop on the Impacts of Aviation on Climate Change

NASA Technical Reports Server (NTRS)

Wuebbles, Don; Gupta, Mohan; Ko, Malcolm

2006-01-01

Projections indicate that demand for aviation transportation will increase by more than two fold over the next few decades. Timely action is needed to understand and quantify the potential climate impacts of aviation emissions particularly given the sustained lapse over the last several years in U.S. research activities in this area. In response to the stated needs, a group of international experts participated in the Workshop on the Impacts of Aviation on Climate Change during June 7-9, 2006 in Boston, MA. The workshop focus was on the impacts of subsonic aircraft emissions in the UT/LS region and on the potential response of the climate system. The goals of the workshop were to assess and document the present state of scientific knowledge, to identify the key underlying uncertainties and gaps, to identify ongoing and further research needed, to explore the development of climate impact metrics, and to help focus the scientific community on the aviation-climate change research needs. The workshop concluded that the major ways that aviation can affect climate, in agreement with the 1999 assessment by the Intergovernmental Panel on Climate Change (IPCC), are the direct climate effects from CO2 and water vapor emissions, the indirect forcing on climate resulting from changes in the distributions and concentrations of ozone and methane as a primary consequence of aircraft nitrogen oxide (NOx) emissions, the direct effects (and indirect effects on clouds) from emitted aerosols and aerosol precursors, and the climate effects associated with contrails and cirrus cloud formation. The workshop was organized in three subgroups: (1) Effects of aircraft emissions on the UT/LS chemical composition, (2) Effects of water and particle emissions on contrails and on cirrus clouds, and (3) Impacts on climate from aircraft emissions and identification of suitable metrics to measure these impacts. The workshop participants acknowledged the need for focused research specifically to address the uncertainties and gaps in our understanding of current and projected impacts of aviation on climate and to develop metrics to better characterize these impacts. This may entail coordination and/or expansion of existing and planned climate research programs, or new activities. Such efforts should include strong and continuing interactions among the science and aviation communities as well as policymakers to develop well-informed decisions.

Three-dimensional variations of atmospheric CO2: aircraft measurements and multi-transport model simulations

NASA Astrophysics Data System (ADS)

Niwa, Y.; Patra, P. K.; Sawa, Y.; Machida, T.; Matsueda, H.; Belikov, D.; Maki, T.; Ikegami, M.; Imasu, R.; Maksyutov, S.; Oda, T.; Satoh, M.; Takigawa, M.

2011-12-01

Numerical simulation and validation of three-dimensional structure of atmospheric carbon dioxide (CO2) is necessary for quantification of transport model uncertainty and its role on surface flux estimation by inverse modeling. Simulations of atmospheric CO2 were performed using four transport models and two sets of surface fluxes compared with an aircraft measurement dataset of Comprehensive Observation Network for Trace gases by AIrLiner (CONTRAIL), covering various latitudes, longitudes, and heights. Under this transport model intercomparison project, spatiotemporal variations of CO2 concentration for 2006-2007 were analyzed with a three-dimensional perspective. Results show that the models reasonably simulated vertical profiles and seasonal variations not only over northern latitude areas but also over the tropics and southern latitudes. From CONTRAIL measurements and model simulations, intrusion of northern CO2 in to the Southern Hemisphere, through the upper troposphere, was confirmed. Furthermore, models well simulated the vertical propagation of seasonal variation in the northern free troposphere. However, significant model-observation discrepancies were found in Asian regions, which are attributable to uncertainty of the surface CO2 flux data. In summer season, differences in latitudinal gradients by the fluxes are comparable to or greater than model-model differences even in the free troposphere. This result suggests that active summer vertical transport sufficiently ventilates flux signals up to the free troposphere and the models could use those for inferring surface CO2 fluxes.

Three-dimensional variations of atmospheric CO2: aircraft measurements and multi-transport model simulations

NASA Astrophysics Data System (ADS)

Niwa, Y.; Patra, P. K.; Sawa, Y.; Machida, T.; Matsueda, H.; Belikov, D.; Maki, T.; Ikegami, M.; Imasu, R.; Maksyutov, S.; Oda, T.; Satoh, M.; Takigawa, M.

2011-04-01

Numerical simulation and validation of three-dimensional structure of atmospheric carbon dioxide (CO2) is necessary for quantification of transport model uncertainty and its role on surface flux estimation by inverse modeling. Simulations of atmospheric CO2 were performed using four transport models and two sets of surface fluxes compared with an aircraft measurement dataset of Comprehensive Observation Network for Trace gases by AIrLiner (CONTRAIL), covering various latitudes, longitudes, and heights. Under this transport model intercomparison project, spatiotemporal variations of CO2 concentration for 2006-2007 were analyzed with a three-dimensional perspective. Results show that the models reasonably simulated vertical profiles and seasonal variations not only over northern latitude areas but also over the tropics and southern latitudes. From CONTRAIL measurements and model simulations, intrusion of northern CO2 in to the Southern Hemisphere, through the upper troposphere, was confirmed. Furthermore, models well simulated the vertical propagation of seasonal variation in the northern free-troposphere. However, significant model-observation discrepancies were found in Asian regions, which are attributable to uncertainty of the surface CO2 flux data. The models consistently underestimated the north-tropics mean gradient of CO2 both in the free-troposphere and marine boundary layer during boreal summer. This result suggests that the north-tropics contrast of annual mean net non-fossil CO2 flux should be greater than 2.7 Pg C yr-1 for 2007.

Global Civil Aviation Black Carbon Particle Mass and Number Emissions

NASA Astrophysics Data System (ADS)

Stettler, M. E. J.

2015-12-01

Black carbon (BC) is a product of incomplete combustion emitted by aircraft engines. In the atmosphere, BC particles strongly absorb incoming solar radiation and influence cloud formation processes leading to highly uncertain, but likely net positive warming of the earth's atmosphere. At cruise altitude, BC particle number emissions can influence the concentration of ice nuclei that can lead to contrail formation, with significant and highly uncertainty climate impacts. BC particles emitted by aircraft engines also degrade air quality in the vicinity of airports and globally. A significant contribution to the uncertainty in environmental impacts of aviation BC emissions is the uncertainty in emissions inventories. Previous work has shown that global aviation BC mass emissions are likely to have been underestimated by a factor of three. In this study, we present an updated global BC particle number inventory and evaluate parameters that contribute to uncertainty using global sensitivity analysis techniques. The method of calculating particle number from mass utilises a description of the mobility of fractal aggregates and uses the geometric mean diameter, geometric standard deviation, mass-mobility exponent, primary particle diameter and material density to relate the particle number concentration to the total mass concentration. Model results show good agreement with existing measurements of aircraft BC emissions at ground level and at cruise altitude. It is hoped that the results of this study can be applied to estimate direct and indirect climate impacts of aviation BC emissions in future studies.

Oblique view of cloud patterns over Pacific Ocean

NASA Image and Video Library

1975-07-16

AST-01-042 (16 July 1975) --- An oblique view of unique cloud patterns over the Pacific Ocean caused by aircraft contrail shadows altering cumulus clouds and forming straight line clouds, as photographed from the Apollo spacecraft in Earth orbit during the joint U.S.-USSR Apollo-Soyuz Test Project mission. This area is southwest of Los Angeles, California. This photograph was taken at an altitude of 177 kilometers (110 statute miles) with a 70mm Hasselblad camera using medium-speed Ektachrome QX-807 type film.

Cloud microstructure studies

NASA Technical Reports Server (NTRS)

Blau, H. H., Jr.; Fowler, M. G.; Chang, D. T.; Ryan, R. T.

1972-01-01

Over two thousand individual cloud droplet size distributions were measured with an optical cloud particle spectrometer flown on the NASA Convair 990 aircraft. Representative droplet spectra and liquid water content, L (gm/cu m) were obtained for oceanic stratiform and cumuliform clouds. For non-precipitating clouds, values of L range from 0.1 gm/cu m to 0.5 gm/cu m; with precipitation, L is often greater than 1 gm/cu m. Measurements were also made in a newly formed contrail and in cirrus clouds.

Greek Islands, Western Asia Minor as seen from STS-58

NASA Technical Reports Server (NTRS)

1993-01-01

This north-looking view shows the western margin of Turkey (right) and the Dodecanese Islands of Greece between the Aegean Sea (left) and the Sea of Crete (foreground). The largest island is Crete (foreground) with the semicircular island of Thira beyond. Thira is dominated by the volcanoe Santorini. Two airplane contrails appear between the Turkish mainland and the large island of Rhodes immediately offshore. The narrow straits of the Dardanelles, joining the Black Sea to the Mediterranean, can be detected top left.

Persister formation in Staphylococcus aureus is associated with ATP depletion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Conlon, Brian P.; Rowe, Sarah E.; Gandt, Autumn Brown

Persisters are dormant phenotypic variants of bacterial cells that are tolerant to killing by antibiotics1. Persisters are associated with chronic bacterial infection and antibiotic treatment failure. In Escherichia coli, toxin/antitoxin (TA) modules are responsible for persister formation. The mechanism of persister formation in Gram positive bacteria is unknown. Staphylococcus aureus is a major human pathogen, responsible for a variety of chronic and relapsing infections such as osteomyelitis, endocarditis and infections of implanted devices. Deleting TA modules in S. aureus did not affect the level of persisters. Here we show that S. aureus persisters are produced due to a stochastic entrancemore » to stationary phase accompanied by a drop in intracellular ATP. Cells expressing stationary state markers are present throughout the growth phase, increasing in frequency with cell density. Cell sorting revealed that expression of stationary markers was associated with a 100-1000 fold increased likelihood of survival to antibiotic challenge. We find that the antibiotic tolerance of these cells is due to a drop in intracellular ATP. The ATP level of the cell is predictive of bactericidal antibiotic efficacy and explains bacterial tolerance to antibiotic treatment.« less

MazEF toxin-antitoxin proteins alter Escherichia coli cell morphology and infrastructure during persister formation and regrowth.

PubMed

Cho, Junho; Carr, Anita Nicole; Whitworth, Lisa; Johnson, Brent; Wilson, Kevin Scott

2017-03-01

When exposed to antibiotics, many bacteria respond by activating intracellular 'toxin' proteins, which arrest cell growth and induce formation of persister cells that survive antibiotics. After antibiotics are removed, persisters can regrow by synthesizing 'antitoxin' proteins that sequester toxin proteins. In Escherichia coli, MazE antitoxin sequesters the activity of MazF toxin, which extensively cleaves cellular RNAs. Although the functions of MazEF proteins are well characterized, there is surprisingly little known about their effects on cell structure. Here, using a combination of microscopy techniques, we visualized the effects of MazEF and three bactericidal antibiotics on E. coli cell morphology and infrastructure. When ectopically expressed in E. coli, MazF temporarily stalled cell growth and induced persister formation, but only mildly elevated DNA mutagenesis. Viewed by electron microscopy, MazF-expressing persister cells were arrested in cell growth and division. Their chromosomal DNAs were compacted into thread-like structures. Their ribosomes were excluded from their nucleoids. After exposure to ciprofloxacin, persister regrowth was activated by MazE. Cell division remained inhibited while cells became extraordinarily elongated, then divided multiple times during stationary growth phase. This extreme filamentation during persister regrowth was unique to ciprofloxacin-treated persisters, likely caused by inhibition of cell division during regrowth, and was not observed with kanamycin-treated persisters.

Wave Clouds over Ireland

NASA Image and Video Library

2017-12-08

Visualization Date 2003-12-18 Clouds ripple over Ireland and Scotland in a wave pattern, similar to the pattern of waves along a seashore. The similarity is not coincidental — the atmosphere behaves like a fluid, so when it encounters an obstacle, it must move around it. This movement forms a wave, and the wave movement can continue for long distances. In this case, the waves were caused by the air moving over and around the mountains of Scotland and Ireland. As the air crested a wave, it cooled, and clouds formed. Then, as the air sank into the trough, the air warmed, and clouds did not form. This pattern repeated itself, with clouds appearing at the peak of every wave. Other types of clouds are also visible in the scene. Along the northwestern and southwestern edges of this true-color image from December 17, 2003, are normal mid-altitude clouds with fairly uniform appearances. High altitude cirrus-clouds float over these, casting their shadows on the lower clouds. Open- and closed-cell clouds formed off the coast of northwestern France, and thin contrail clouds are visible just east of these. Contrail clouds form around the particles carried in airplane exhaust. Fog is also visible in the valleys east of the Cambrian Mountains, along the border between northern/central Wales and England. This is an Aqua MODIS image. Sensor Aqua/MODIS Credit Jacques Descloitres, MODIS Rapid Response Team, NASA/GSFC For more information go to: visibleearth.nasa.gov/view_rec.php?id=6146

Persistence-Retention. Snapshot™ Report, Spring 2014

ERIC Educational Resources Information Center

National Student Clearinghouse, 2014

2014-01-01

This snapshot report provides information on student persistence and retention rates for Spring 2014. Data is presented in tabular format on the following: (1) First-Year Persistence and Retention Rates by Starting Enrollment Intensity (all institutional sectors); (2) First-Year Persistence and Retention Rates by Age at College Entry (all…

The Extraction of Information From Visual Persistence

ERIC Educational Resources Information Center

Erwin, Donald E.

1976-01-01

This research sought to distinguish among three concepts of visual persistence by substituting the physical presence of the target stimulus while simultaneously inhibiting the formation of a persisting representation. Reportability of information about the stimuli was compared to a condition in which visual persistence was allowed to fully develop…

Instrumentation for Aerosol and Gas Speciation

NASA Technical Reports Server (NTRS)

Coggiola, Michael J.

1998-01-01

Using support from NASA Grant No. NAG 2-963, SRI International successfully completed the project, entitled, 'Instrumentation for Aerosol and Gas Speciation.' This effort (SRI Project 7383) covered the design, fabrication, testing, and deployment of a real-time aerosol speciation instrument in NASA's DC-8 aircraft during the Spring 1996 SUbsonic aircraft: Contrail and Cloud Effects Special Study (SUCCESS) mission. This final technical report describes the pertinent details of the instrument design, its abilities, its deployment during SUCCESS and the data acquired from the mission, and the post-mission calibration, data reduction, and analysis.

A search for life on earth at 100 meter resolution.

NASA Technical Reports Server (NTRS)

Sagan, C.; Wallace, D.

1971-01-01

Study of several thousand photos indicating that about 0.01 of Gemini and Apollo photographs of the earth at 100 m resolution reveal signs of life - rectangular arrays due to human agricultural and urban territoriality, roads, canals, jet contrails, and industrial pollution. Potential false positives - e.g., dunes, sand bars, jetstream clouds - abound. A curve is derived for the detectivity of contemporary life on earth, in a plot of ground resolution versus global coverage. A comparable biology on Mars would not have been detected by all observations of Mars through Mariner 7.

NASA DC-8 Airborne Scanning Lidar Sensor Development

NASA Technical Reports Server (NTRS)

Nielsen, Norman B.; Uthe, Edward E.; Kaiser, Robert D.; Tucker, Michael A.; Baloun, James E.; Gorordo, Javier G.

1996-01-01

The NASA DC-8 aircraft is used to support a variety of in-situ and remote sensors for conducting environmental measurements over global regions. As part of the atmospheric effects of aviation program (AEAP) the DC-8 is scheduled to conduct atmospheric aerosol and gas chemistry and radiation measurements of subsonic aircraft contrails and cirrus clouds. A scanning lidar system is being developed for installation on the DC-8 to support and extend the domain of the AEAP measurements. Design and objectives of the DC-8 scanning lidar are presented.

A common mechanism involving the TORC1 pathway can lead to amphotericin B-persistence in biofilm and planktonic Saccharomyces cerevisiae populations.

PubMed

Bojsen, Rasmus; Regenberg, Birgitte; Gresham, David; Folkesson, Anders

2016-02-23

Fungal infections are an increasing clinical problem. Decreased treatment effectiveness is associated with biofilm formation and drug recalcitrance is thought to be biofilm specific. However, no systematic investigations have tested whether resistance mechanisms are shared between biofilm and planktonic populations. We performed multiplexed barcode sequencing (Bar-seq) screening of a pooled collection of gene-deletion mutants cultivated as biofilm and planktonic cells. Screening for resistance to the ergosterol-targeting fungicide amphotericin B (AmB) revealed that the two growth modes had significant overlap in AmB-persistent mutants. Mutants defective in sterol metabolism, ribosome biosynthesis, and the TORC1 and Ras pathways showed increased persistence when treated with AmB. The ras1, ras2 and tor1 mutants had a high-persister phenotype similar to wild-type biofilm and planktonic cells exposed to the TORC1 pathway inhibitor rapamycin. Inhibition of TORC1 with rapamycin also increased the proportion of persisters in Candida albicans and Candida glabrata. We propose that decreased TORC1-mediated induction of ribosome biosynthesis via Ras can lead to formation of AmB-persister cells regardless of whether the cells are in planktonic or biofilm growth mode. Identification of common pathways leading to growth mode-independent persister formation is important for developing novel strategies for treating fungal infections.

(p)ppGpp-Dependent Persisters Increase the Fitness of Escherichia coli Bacteria Deficient in Isoaspartyl Protein Repair.

PubMed

VandenBerg, Kelsey E; Ahn, Sarah; Visick, Jonathan E

2016-09-01

The l-isoaspartyl protein carboxyl methyltransferase (PCM) repairs protein damage resulting from spontaneous conversion of aspartyl or asparaginyl residues to isoaspartate and increases long-term stationary-phase survival of Escherichia coli under stress. In the course of studies intended to examine PCM function in metabolically inactive cells, we identified pcm as a gene whose mutation influences the formation of ofloxacin-tolerant persisters. Specifically, a Δpcm mutant produced persisters for an extended period in stationary phase, and a ΔglpD mutation drastically increased persisters in a Δpcm background, reaching 23% of viable cells. The high-persister double mutant showed much higher competitive fitness than the pcm mutant in competition with wild type during long-term stationary phase, suggesting a link between persistence and the mitigation of unrepaired protein damage. We hypothesized that reduced metabolism in the high-persister strain might retard protein damage but observed no gross differences in metabolism relative to wild-type or single-mutant strains. However, methylglyoxal, which accumulates in glpD mutants, also increased fitness, suggesting a possible mechanism. High-level persister formation in the Δpcm ΔglpD mutant was dependent on guanosine pentaphosphate [(p)ppGpp] and polyphosphate. In contrast, persister formation in the Δpcm mutant was (p)ppGpp independent and thus may occur by a distinct pathway. We also observed an increase in conformationally unstable proteins in the high-persister strain and discuss this as a possible trigger for persistence as a response to unrepaired protein damage. Protein damage is an important factor in the survival and function of cells and organisms. One specific form of protein damage, the formation of the abnormal amino acid isoaspartate, can be repaired by a nearly universally conserved enzyme, PCM. PCM-directed repair is associated with stress survival and longevity in bacteria, insects, worms, plants, mice, and humans, but much remains to be learned about the specific effects of protein damage and repair. This paper identifies an unexpected connection between isoaspartyl protein damage and persisters, subpopulations in bacterial cultures showing increased tolerance to antibiotics. In the absence of PCM, the persister population in Escherichia coli bacteria increased, especially if the metabolic gene glpD was also mutated. High levels of persisters in pcm glpD double mutants correlated with increased fitness of the bacteria in a competition assay, and the fitness was dependent on the signal molecule (p)ppGpp; this may represent an alternative pathway for responding to protein damage. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

FORMATION AND PERSISTANCE OF DNA ADDUCTS IN THE LIVER OF BROWN BULLHEADS EXPOSED TO BENZO(A)PYRENE

EPA Science Inventory

The formation and persistence of benzo[a]pyrene (BP)-DNA adducts in the liver of brown bullheads (Ictalurus nebulosus) treated with the hydrocarbon (20 mg/kg body wt, i.p.) was investigated using the 32P-postlabeling assay. he highest level of covalent binding of BP to liver DNA ...

Prophages and Growth Dynamics Confound Experimental Results with Antibiotic-Tolerant Persister Cells

PubMed Central

Fino, Cinzia; Sørensen, Michael A.; Semsey, Szabolcs

2017-01-01

ABSTRACT Bacterial persisters are phenotypic variants that survive antibiotic treatment in a dormant state and can be formed by multiple pathways. We recently proposed that the second messenger (p)ppGpp drives Escherichia coli persister formation through protease Lon and activation of toxin-antitoxin (TA) modules. This model found considerable support among researchers studying persisters but also generated controversy as part of recent debates in the field. In this study, we therefore used our previous work as a model to critically examine common experimental procedures to understand and overcome the inconsistencies often observed between results of different laboratories. Our results show that seemingly simple antibiotic killing assays are very sensitive to variations in culture conditions and bacterial growth phase. Additionally, we found that some assay conditions cause the killing of antibiotic-tolerant persisters via induction of cryptic prophages. Similarly, the inadvertent infection of mutant strains with bacteriophage ϕ80, a notorious laboratory contaminant, apparently caused several of the phenotypes that we reported in our previous studies. We therefore reconstructed all infected mutants and probed the validity of our model of persister formation in a refined assay setup that uses robust culture conditions and unravels the dynamics of persister cells through all bacterial growth stages. Our results confirm the importance of (p)ppGpp and Lon but no longer support a role of TA modules in E. coli persister formation under unstressed conditions. We anticipate that the results and approaches reported in our study will lay the ground for future work in the field. PMID:29233898

Stromal cells in chronic inflammation and tertiary lymphoid organ formation.

PubMed

Buckley, Christopher D; Barone, Francesca; Nayar, Saba; Bénézech, Cecile; Caamaño, Jorge

2015-01-01

Inflammation is an unstable state. It either resolves or persists. Why inflammation persists and the factors that define tissue tropism remain obscure. Increasing evidence suggests that tissue-resident stromal cells not only provide positional memory but also actively regulate the differential accumulation of inflammatory cells within inflamed tissues. Furthermore, at many sites of chronic inflammation, structures that mimic secondary lymphoid tissues are observed, suggesting that chronic inflammation and lymphoid tissue formation share common activation programs. Similarly, blood and lymphatic endothelial cells contribute to tissue homeostasis and disease persistence in chronic inflammation. This review highlights our increasing understanding of the role of stromal cells in inflammation and summarizes the novel immunological role that stromal cells exert in the persistence of inflammatory diseases.

Characterization and Transcriptome Analysis of Acinetobacter baumannii Persister Cells.

PubMed

Alkasir, Rashad; Ma, Yanan; Liu, Fei; Li, Jing; Lv, Na; Xue, Yong; Hu, Yongfei; Zhu, Baoli

2018-06-14

Acinetobacter baumannii is a nonfermenting Gram-negative bacillus. A. baumannii resistance is a significant obstacle to clinical infection treatment. The existence of persister cells (persisters) might represent the reason for therapy failure and relapse, and such cells may be the driving force behind rising resistance rates. In this study, A. baumannii ATCC 19606 was used as a target to explore the essential features of A. baumannii persisters. Antibiotic treatment of A. baumannii cultures at 50-fold the minimum inhibitory concentration resulted in a distinct plateau of surviving drug-tolerant persisters. The sensitive bacteria were lysed with ceftazidime, and the nonreplicating bacteria were isolated for transcriptome analysis using RNA sequencing. We analyzed the transcriptome of A. baumannii persisters and identified significantly differentially expressed genes, as well as their enriched pathways. The results showed that both the GP49 (HigB)/Cro (HigA) and DUF1044/RelB toxin/antitoxin systems were significantly increased during the persister incubation period. In addition, the activities of certain metabolic pathways (such as electron transport, adenosine triphosphate [ATP], and the citrate cycle) decreased sharply after antibiotic treatment and remained low during the persister period, while aromatic compound degradation genes were only upregulated in persisters. These results suggest the involvement of aromatic compound degradation genes in persister formation and maintenance. They further provide the first insight into the mechanism of persister formation in A. baumannii.

Fumarate-Mediated Persistence of Escherichia coli against Antibiotics

PubMed Central

Kim, Jun-Seob; Cho, Da-Hyeong; Heo, Paul; Jung, Suk-Chae; Park, Myungseo; Oh, Eun-Joong; Sung, Jaeyun; Kim, Pan-Jun; Lee, Suk-Chan; Lee, Dae-Hee; Lee, Sarah; Lee, Choong Hwan; Shin, Dongwoo

2016-01-01

Bacterial persisters are a small fraction of quiescent cells that survive in the presence of lethal concentrations of antibiotics. They can regrow to give rise to a new population that has the same vulnerability to the antibiotics as did the parental population. Although formation of bacterial persisters in the presence of various antibiotics has been documented, the molecular mechanisms by which these persisters tolerate the antibiotics are still controversial. We found that amplification of the fumarate reductase operon (FRD) in Escherichia coli led to a higher frequency of persister formation. The persister frequency of E. coli was increased when the cells contained elevated levels of intracellular fumarate. Genetic perturbations of the electron transport chain (ETC), a metabolite supplementation assay, and even the toxin-antitoxin-related hipA7 mutation indicated that surplus fumarate markedly elevated the E. coli persister frequency. An E. coli strain lacking succinate dehydrogenase (SDH), thereby showing a lower intracellular fumarate concentration, was killed ∼1,000-fold more effectively than the wild-type strain in the stationary phase. It appears that SDH and FRD represent a paired system that gives rise to and maintains E. coli persisters by producing and utilizing fumarate, respectively. PMID:26810657

STS-131 Discovery Launch

NASA Image and Video Library

2010-04-04

Contrails are seen as workers leave the Launch Control Center after the launch of the space shuttle Discovery and the start of the STS-131 mission at NASA Kennedy Space Center in Cape Canaveral, Fla. on Monday April 5, 2010. Discovery is carrying a multi-purpose logistics module filled with science racks for the laboratories aboard the station. The mission has three planned spacewalks, with work to include replacing an ammonia tank assembly, retrieving a Japanese experiment from the station’s exterior, and switching out a rate gyro assembly on the station’s truss structure. Photo Credit: (NASA/Bill Ingalls)

STS-28 Mission Insignia

NASA Technical Reports Server (NTRS)

1988-01-01

The STS-28 insignia was designed by the astronaut crew, who said it portrays the pride the American people have in their manned spaceflight program. It depicts America (the eagle) guiding the space program (the Space Shuttle) safely home from an orbital mission. The view looks south on Baja California and the west coast of the United States as the space travelers re-enter the atmosphere. The hypersonic contrails created by the eagle and Shuttle represent the American flag. The crew called the simple boldness of the design symbolic of America's unfaltering commitment to leadership in the exploration and development of space.

Space Shuttle Projects

NASA Image and Video Library

1988-11-07

The STS-28 insignia was designed by the astronaut crew, who said it portrays the pride the American people have in their manned spaceflight program. It depicts America (the eagle) guiding the space program (the Space Shuttle) safely home from an orbital mission. The view looks south on Baja California and the west coast of the United States as the space travelers re-enter the atmosphere. The hypersonic contrails created by the eagle and Shuttle represent the American flag. The crew called the simple boldness of the design symbolic of America's unfaltering commitment to leadership in the exploration and development of space.

Estimating Asian terrestrial carbon fluxes from CONTRAIL aircraft and surface CO2 observations for the period 2006-2010

NASA Astrophysics Data System (ADS)

Zhang, H. F.; Chen, B. Z.; Machida, T.; Matsueda, H.; Sawa, Y.; Fukuyama, Y.; Langenfelds, R.; van der Schoot, M.; Xu, G.; Yan, J. W.; Cheng, M. L.; Zhou, L. X.; Tans, P. P.; Peters, W.

2014-06-01

Current estimates of the terrestrial carbon fluxes in Asia show large uncertainties particularly in the boreal and mid-latitudes and in China. In this paper, we present an updated carbon flux estimate for Asia ("Asia" refers to lands as far west as the Urals and is divided into boreal Eurasia, temperate Eurasia and tropical Asia based on TransCom regions) by introducing aircraft CO2 measurements from the CONTRAIL (Comprehensive Observation Network for Trace gases by Airline) program into an inversion modeling system based on the CarbonTracker framework. We estimated the averaged annual total Asian terrestrial land CO2 sink was about -1.56 Pg C yr-1 over the period 2006-2010, which offsets about one-third of the fossil fuel emission from Asia (+4.15 Pg C yr-1). The uncertainty of the terrestrial uptake estimate was derived from a set of sensitivity tests and ranged from -1.07 to -1.80 Pg C yr-1, comparable to the formal Gaussian error of ±1.18 Pg C yr-1 (1-sigma). The largest sink was found in forests, predominantly in coniferous forests (-0.64 ± 0.70 Pg C yr-1) and mixed forests (-0.14 ± 0.27 Pg C yr-1); and the second and third large carbon sinks were found in grass/shrub lands and croplands, accounting for -0.44 ± 0.48 Pg C yr-1 and -0.20 ± 0.48 Pg C yr-1, respectively. The carbon fluxes per ecosystem type have large a priori Gaussian uncertainties, and the reduction of uncertainty based on assimilation of sparse observations over Asia is modest (8.7-25.5%) for most individual ecosystems. The ecosystem flux adjustments follow the detailed a priori spatial patterns by design, which further increases the reliance on the a priori biosphere exchange model. The peak-to-peak amplitude of inter-annual variability (IAV) was 0.57 Pg C yr-1 ranging from -1.71 Pg C yr-1 to -2.28 Pg C yr-1. The IAV analysis reveals that the Asian CO2 sink was sensitive to climate variations, with the lowest uptake in 2010 concurrent with a summer flood and autumn drought and the largest CO2 sink in 2009 owing to favorable temperature and plentiful precipitation conditions. We also found the inclusion of the CONTRAIL data in the inversion modeling system reduced the uncertainty by 11% over the whole Asian region, with a large reduction in the southeast of boreal Eurasia, southeast of temperate Eurasia and most tropical Asian areas.

Characterization of Aircraft Produced Soot and Contrails Near the Tropopause

NASA Technical Reports Server (NTRS)

Hallett, John; Gudson, James G.

1997-01-01

Participation in the SUCCESS project primarily involved development and deployment of specific instruments for characterizing jet aircraft exhaust emissions as particulates and their subsequent evolution as contrail particles, either liquid or solid, as cirrus. Observations can be conveniently considered in two categories - close or distant from the aircraft. Thus close to the aircraft the exhaust is mixing through the engine turbulence with a much drier and colder environment and developing water/ ice supersaturation along the trail depending on circumstances (near field), whereas distant from the aircraft (far field) the exhaust has cooled essentially to ambient temperature, the turbulence has decayed and any particle growth or evaporation is controlled by the prevailing ambient conditions. Intermediate between these two regions the main aircraft vortices form (one on each side of the aircraft) which tend to inhibit mixing under some conditions, a region extending from a few aircraft lengths to sometimes a hundred times this distance. Our approach to the problem lay in experience gained in characterizing the smoke from hydrocarbon combustion in terms of its cloud forming properties and its potential influence on the radiation properties of the smoke and subsequent cloud from the viewpoint of reduction (absorbtion and scattering ) of solar radiation flux leading to significant global cooling (Hudson et al 1991; Hallett and Hudson 1991). Engine exhaust contains a much smaller proportion of the fuel carbon than is sometimes present in ordinary combustion (less than 0.01% compared with 10%) and influences condensation in quite different ways, to be characterized by the Cloud Condensation Nucleus, CCN - supersaturation spectrum. The transition to ice is to be related to the dilution of solution droplets to freeze by homogeneous nucleation at temperatures somewhat below -40C (Pueschel et al 1998). The subsequent growth of ice particles depends critically on temperature, supersaturation and to some extent pressure, as is demonstrated in an NSF funded project being carried out in parallel with the work reported here. As will be discussed below, nucleation processes themselves and also exhaust impurities also influence the growth of ice particles and may control some aspects of growth of ice in contrails. Instrumentation was designed to give insight into these questions and to be flown on the NASA DC- 8 as a platform. In addition a modest program was undertaken to investigate the properties of laboratory produced smoke produced under controlled conditions from the viewpoint of forming both CCN and CN. The composition of the smoke could inferred from a thermal characterization technique; larger particles were captured by formvar replicator for detailed analysis; ice particles were captured and evaporated in flight on a new instrument, the cloudscope, to give their mass, density and impurity content.

The Role of Persistence at Preschool Age in Academic Skills at Kindergarten

ERIC Educational Resources Information Center

Mokrova, Irina L.; O'Brien, Marion; Calkins, Susan D.; Leerkes, Esther M.; Marcovitch, Stuart

2013-01-01

The current study examined the role of preschoolers' motivation, operationalized as persistence, in the formation of language and math skills at kindergarten. The participants were 263 children from diverse ethnic and socio-economic backgrounds. Demographic information, child persistence, and early cognitive-linguistic skills were assessed at…

Biofilm formation by enteric pathogens and its role in plant colonization and persistence

PubMed Central

Yaron, Sima; Römling, Ute

2014-01-01

The significant increase in foodborne outbreaks caused by contaminated fresh produce, such as alfalfa sprouts, lettuce, melons, tomatoes and spinach, during the last 30 years stimulated investigation of the mechanisms of persistence of human pathogens on plants. Emerging evidence suggests that Salmonella enterica and Escherichia coli, which cause the vast majority of fresh produce outbreaks, are able to adhere to and to form biofilms on plants leading to persistence and resistance to disinfection treatments, which subsequently can cause human infections and major outbreaks. In this review, we present the current knowledge about host, bacterial and environmental factors that affect the attachment to plant tissue and the process of biofilm formation by S. enterica and E. coli, and discuss how biofilm formation assists in persistence of pathogens on the plants. Mechanisms used by S. enterica and E. coli to adhere and persist on abiotic surfaces and mammalian cells are partially similar and also used by plant pathogens and symbionts. For example, amyloid curli fimbriae, part of the extracellular matrix of biofilms, frequently contribute to adherence and are upregulated upon adherence and colonization of plant material. Also the major exopolysaccharide of the biofilm matrix, cellulose, is an adherence factor not only of S. enterica and E. coli, but also of plant symbionts and pathogens. Plants, on the other hand, respond to colonization by enteric pathogens with a variety of defence mechanisms, some of which can effectively inhibit biofilm formation. Consequently, plant compounds might be investigated for promising novel antibiofilm strategies. PMID:25351039

Persistent Step-Flow Growth of Strained Films on Vicinal Substrates

NASA Astrophysics Data System (ADS)

Hong, Wei; Lee, Ho Nyung; Yoon, Mina; Christen, Hans M.; Lowndes, Douglas H.; Suo, Zhigang; Zhang, Zhenyu

2005-08-01

We propose a model of persistent step flow, emphasizing dominant kinetic processes and strain effects. Within this model, we construct a morphological phase diagram, delineating a regime of step flow from regimes of step bunching and island formation. In particular, we predict the existence of concurrent step bunching and island formation, a new growth mode that competes with step flow for phase space, and show that the deposition flux and temperature must be chosen within a window in order to achieve persistent step flow. The model rationalizes the diverse growth modes observed in pulsed laser deposition of SrRuO3 on SrTiO3.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Devereux, Nick, E-mail: devereux@erau.edu

Dual epoch spectroscopy of the lenticular galaxy, NGC 4203, obtained with the Hubble Space Telescope has revealed that the double-peaked component of the broad H{alpha} emission line is time variable, increasing by a factor of 2.2 in brightness between 1999 and 2010. Modeling the gas distribution responsible for the double-peaked profiles indicates that a ring is a more appropriate description than a disk and most likely represents the contrail of a red supergiant star that is being tidally disrupted at a distance of {approx}1500 AU from the central black hole. There is also a bright core of broad H{alpha} linemore » emission that is not time variable and identified with a large-scale inflow from an outer radius of {approx}1 pc. If the gas number density is {>=}10{sup 6} cm{sup -3}, as suggested by the absence of similarly broad [O I] and [O III] emission lines, then the steady state inflow rate is {approx} 2 Multiplication-Sign 10{sup -2} M{sub Sun} yr{sup -1}, which exceeds the inflow requirement to explain the X-ray luminosity in terms of radiatively inefficient accretion by a factor of {approx}6. The central active galactic nucleus (AGN) is unable to sustain ionization of the broad-line region; the discrepancy is particularly acute in 2010 when the broad H{alpha} emission line is dominated by the contrail of the infalling supergiant star. However, ram pressure shock ionization produced by the interaction of the infalling supergiant with the ambient interstellar medium may help alleviate the ionizing deficit by generating a mechanical source of ionization supplementing the photoionization provided by the AGN.« less

Effects of the swimming exercise on the consolidation and persistence of auditory and contextual fear memory.

PubMed

Faria, Rodolfo Souza; Gutierres, Luís Felipe Soares; Sobrinho, Fernando César Faria; Miranda, Iris do Vale; Reis, Júlia Dos; Dias, Elayne Vieira; Sartori, Cesar Renato; Moreira, Dalmo Antonio Ribeiro

2016-08-15

Exposure to negative environmental events triggers defensive behavior and leads to the formation of aversive associative memory. Cellular and molecular changes in the central nervous system underlie this memory formation, as well as the associated behavioral changes. In general, memory process is established in distinct phases such as acquisition, consolidation, evocation, persistence, and extinction of the acquired information. After exposure to a particular event, early changes in involved neural circuits support the memory consolidation, which corresponds to the short-term memory. Re-exposure to previously memorized events evokes the original memory, a process that is considered essential for the reactivation and consequent persistence of memory, ensuring that long-term memory is established. Different environmental stimuli may modulate the memory formation process, as well as their distinct phases. Among the different environmental stimuli able of modulating memory formation is the physical exercise which is a potent modulator of neuronal activity. There are many studies showing that physical exercise modulates learning and memory processes, mainly in the consolidation phase of the explicit memory. However, there are few reports in the literature regarding the role of physical exercise in implicit aversive associative memory, especially at the persistence phase. Thus, the present study aimed to investigate the relationship between swimming exercise and the consolidation and persistence of contextual and auditory-cued fear memory. Male Wistar rats were submitted to sessions of swimming exercise five times a week, over six weeks. After that, the rats were submitted to classical aversive conditioning training by a pairing tone/foot shock paradigm. Finally, rats were evaluated for consolidation and persistence of fear memory to both auditory and contextual cues. Our results demonstrate that classical aversive conditioning with tone/foot shock pairing induced consolidation as well as persistence of conditioned fear memory. In addition, rats submitted to swimming exercise over six weeks showed an improved performance in the test of auditory-cued fear memory persistence, but not in the test of contextual fear memory persistence. Moreover, no significant effect from swimming exercise was observed on consolidation of both contextual and auditory fear memory. So, our study, revealing the effect of the swimming exercise on different stages of implicit memory of tone/foot shock conditioning, contributes to and complements the current knowledge about the environmental modulation of memory process. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Persister eradication: lessons from the world of natural products.

PubMed

Keren, Iris; Mulcahy, Lawrence R; Lewis, Kim

2012-01-01

Persisters are specialized survivor cells that protect bacterial populations from killing by antibiotics. Persisters are dormant phenotypic variants of regular cells rather than mutants. Bactericidal antibiotics kill by corrupting their targets into producing toxic products; tolerance to antibiotics follows when targets are inactive. Transcriptome analysis of isolated persisters points to toxin/antitoxin modules as a principle component of persister formation. Mechanisms of persister formation are redundant, making it difficult to eradicate these cells. In Escherichia coli, toxins RelE and MazF cause dormancy by degrading mRNA; HipA inhibits translation by phosphorylating Ef-Tu; and TisB forms an anion channel in the membrane, leading to a decrease in pmf and ATP levels. Prolonged treatment of chronic infections with antibiotics selects for hip mutants that produce more persister cells. Eradication of tolerant persisters is a serious challenge. Some of the existing antibiotics are capable of killing persisters, pointing to ways of developing therapeutics to treat chronic infections. Mitomycin is a prodrug which is converted into a reactive compound forming adducts with DNA upon entering the cell. Prolonged treatment with aminoglycosides that cause mistranslation leading to misfolded peptides can sterilize a stationary culture of Pseudomonas aeruginosa, a pathogen responsible for chronic, highly tolerant infections of cystic fibrosis patients. Finally, one of the best bactericidal agents is rifampin, an inhibitor of RNA polymerase, and we suggest that it "kills" by preventing persister resuscitation. Copyright © 2012 Elsevier Inc. All rights reserved.

Nontypeable Haemophilus influenzae initiates formation of neutrophil extracellular traps.

PubMed

Juneau, Richard A; Pang, Bing; Weimer, Kristin E D; Armbruster, Chelsie E; Swords, W Edward

2011-01-01

Nontypeable Haemophilus influenzae (NTHI) is a leading cause of otitis media infections, which are often chronic and/or recurrent in nature. NTHI and other bacterial species persist in vivo within biofilms during otitis media and other persistent infections. These biofilms have a significant host component that includes neutrophil extracellular traps (NETs). These NETs do not mediate clearance of NTHI, which survives within NET structures by means of specific subpopulations of lipooligosaccharides on the bacterial surface that are determinants of biofilm formation in vitro. In this study, the ability of NTHI and NTHI components to initiate NET formation was examined using an in vitro model system. Both viable and nonviable NTHI strains were shown to promote NET formation, as did preparations of bacterial DNA, outer membrane proteins, and lipooligosaccharide (endotoxin). However, only endotoxin from a parental strain of NTHI exhibited equivalent potency in NET formation to that of NTHI. Additional studies showed that NTHI entrapped within NET structures is resistant to both extracellular killing within NETs and phagocytic killing by incoming neutrophils, due to oligosaccharide moieties within the lipooligosaccharides. Thus, we concluded that NTHI elicits NET formation by means of multiple pathogen-associated molecular patterns (most notably endotoxin) and is highly resistant to killing within NET structures. These data support the conclusion that, for NTHI, formation of NET structures may be a persistence determinant by providing a niche within the middle-ear chamber.

Probabilistic description of ice-supersaturated layers in low resolution profiles of relative humidity

NASA Astrophysics Data System (ADS)

Dickson, N. C.; Gierens, K. M.; Rogers, H. L.; Jones, R. L.

2010-02-01

The global observation, assimilation and prediction in numerical models of ice super-saturated (ISS) regions (ISSR) are crucial if the climate impact of aircraft condensations trails (contrails) is to be fully understood, and if, for example, contrail formation is to be avoided through aircraft operational measures. A robust assessment of the global distribution of ISSR will further this debate, and ISS event occurrence, frequency and spatial scales have recently attracted significant attention. The mean horizontal path length through ISSR as observed by MOZAIC aircraft is 150 km (±250 km). The average vertical thickness of ISS layers is 600-800 m (±575 m) but layers ranging from 25 m to 3000 m have been observed, with up to one third of ISS layers thought to be less than 100 m deep. Given their small scales compared to typical atmospheric model grid sizes, statistical representations of the spatial scales of ISSR are required, in both horizontal and vertical dimensions, if global occurrence of ISSR is to be adequately represented in climate models. This paper uses radiosonde launches made by the UK Meteorological Office, from the British Isles, Gibraltar, St. Helena and the Falkland Islands between January 2002 and December 2006, to investigate the probabilistic occurrence of ISSR. Specifically each radiosonde profile is divided into 50- and 100-hPa pressure layers, to emulate the coarse vertical resolution of some atmospheric models. Then the high resolution observations contained within each thick pressure layer are used to calculate an average relative humidity and an ISS fraction for each individual thick pressure layer. These relative humidity pressure layer descriptions are then linked through a probability function to produce an s-shaped curve describing the ISS fraction in any average relative humidity pressure layer. An empirical investigation has shown that this one curve is statistically valid for mid-latitude locations, irrespective of season and altitude, however, pressure layer depth is an important variable. Using this empirical understanding of the s-shaped relationship a mathematical model was developed to represent the ISS fraction within any arbitrary thick pressure layer. Here the statistical distributions of actual high resolution RHi observations in any thick pressure layer, along with an error function, are used to mathematically describe the s-shape. Two models were developed to represent both 50- and 100-hPa pressure layers with each reconstructing their respective s-shapes within 8-10% of the empirical curves. These new models can be used, to represent the small scale structures of ISS events, in modelled data where only low vertical resolution is available. This will be useful in understanding, and improving the global distribution, both observed and forecasted, of ice super-saturation.

Breathing Cleaner Fumes

NASA Image and Video Library

2014-10-14

There’s more than one way to study the impact of biofuels. For NASA scientists, it means trailing an aircraft from as little as 300 feet behind while flying 34,000 feet in the air. Earlier this year, a NASA-led team conducted a series of carefully choreographed flights over California in order to sniff out how aircraft emissions differ when using petroleum fuels or biofuels. Early results from the Alternative Fuel Effects on Contrails and Cruise Emissions (ACCESS II) experiment confirm that blended biofuel is the cleaner-burning fuel. "Our findings show we definitely see a 50 percent reduction in soot emissions from the DC-8 when it burns the blended fuel as opposed to jet fuel alone," said Bruce Anderson, ACCESS principal investigator from NASA's Langley Research Center. The DC-8 is a NASA science workhorse: a flying laboratory equipped to collect—or, in this case, produce—data for basic Earth science research. During the ACESS experiment, scientists took advantage of the aircraft's segregated fuel tank. On the fly, the pilot switched the fuel type sent to each of the four engines. The engines burned either jet fuel, or a 50-50 blend of jet fuel and a renewable alternative produced from camelina plant oil. With each change of fuel, three other instrumented aircraft took turns lining up in the DC-8's wake and flying anywhere from 90 meters (300 feet) to more than 30 kilometers (20 miles) behind to catch a sniff. Richard Moore, a post-doctoral fellow at NASA Langley, took this photograph with a DSLR camera on May 7, 2014, during an ACCESS II test flight over Edwards Air Force Base in California. The photo was taken from Langley's HU-25C Guardian jet as it descended toward NASA's Armstrong Flight Research Center after a successful three-hour sampling flight behind the DC-8. The aircraft trailing the DC-8 in the photo was a Falcon 20-E5 jet owned by the German Aerospace Center. The flight on May 7 was just the first in a series of flights that lasted throughout the month. After the campaign, researchers continued to examine the data to determine whether a reduction in soot emissions translates to a reduction in contrail formation, and how that might affect climate. Read more: 1.usa.gov/1wBfaKq NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

SUCCESS Evidence for Cirrus Cloud Ice Nucleation Mechanisms

NASA Technical Reports Server (NTRS)

Jensen, Eric; Gore, Warren J. Y. (Technical Monitor)

1997-01-01

During the SUCCESS mission, several measurements were made which should improve our understanding of ice nucleation processes in cirrus clouds. Temperature and water vapor concentration were made with a variety of instruments on the NASA DC-8. These observations should provide accurate upper tropospheric humidities. In particular, we will evaluate what humidities are required for ice nucleation. Preliminary results suggest that substantial supersaturations frequently exist in the upper troposphere. The leading-edge region of wave-clouds (where ice nucleation occurs) was sampled extensively at temperatures near -40 and -60C. These observations should give precise information about conditions required for ice nucleation. In addition, we will relate the observed aerosol composition and size distributions to the ice formation observed to evaluate the role of soot or mineral particles on ice nucleation. As an alternative technique for determining what particles act as ice nuclei, numerous samples of aerosols inside ice crystals were taken. In some cases, large numbers of aerosols were detected in each crystal, indicating that efficient scavenging occurred. Analysis of aerosols in ice crystals when only one particle per crystal was detected should help with the ice nucleation issue. Direct measurements of the ice nucleating activity of ambient aerosols drawn into airborne cloud chambers were also made. Finally, measurements of aerosols and ice crystals in contrails should indicate whether aircraft exhaust soot particles are effective ice nuclei.

STS-108 Endeavour Launch from Pad 39-B

NASA Technical Reports Server (NTRS)

2001-01-01

STS-108 Endeavour Launch from Pad 39-B KSC-01PD-1786 KENNEDY SPACE CENTER, Fla. -- Like a lighted taper, Space Shuttle Endeavour shines atop its twisted contrail as it soars into space on mission STS-108. Liftoff occurred at 5:19:28 p.m. EST (10:19.28 GMT). Endeavour will dock with the International Space Station on Dec. 7. STS-108 is the final Shuttle mission of 2001and the 107th Shuttle flight overall. It is the 12th flight to the Space Station. Landing of the orbiter at KSC's Shuttle Landing Facility is targeted for 1:05 p.m. EST (6:05 p.m. GMT) Dec. 16.

Does stress remove the HDAC brakes for the formation and persistence of long-term memory?

PubMed

White, André O; Wood, Marcelo A

2014-07-01

It has been known for numerous decades that gene expression is required for long-lasting forms of memory. In the past decade, the study of epigenetic mechanisms in memory processes has revealed yet another layer of complexity in the regulation of gene expression. Epigenetic mechanisms do not only provide complexity in the protein regulatory complexes that control coordinate transcription for specific cell function, but the epigenome encodes critical information that integrates experience and cellular history for specific cell functions as well. Thus, epigenetic mechanisms provide a unique mechanism of gene expression regulation for memory processes. This may be why critical negative regulators of gene expression, such as histone deacetylases (HDACs), have powerful effects on the formation and persistence of memory. For example, HDAC inhibition has been shown to transform a subthreshold learning event into robust long-term memory and also generate a form of long-term memory that persists beyond the point at which normal long-term memory fails. A key question that is explored in this review, from a learning and memory perspective, is whether stress-dependent signaling drives the formation and persistence of long-term memory via HDAC-dependent mechanisms. Copyright © 2013 Elsevier Inc. All rights reserved.

Does stress remove the HDAC brakes for the formation and persistence of long-term memory?

PubMed Central

White, André O.; Wood, Marcelo A.

2013-01-01

It has been known for numerous decades that gene expression is required for long-lasting forms of memory. In the past decade, the study of epigenetic mechanisms in memory processes has revealed yet another layer of complexity in the regulation of gene expression. Epigenetic mechanisms do not only provide complexity in the protein regulatory complexes that control coordinate transcription for specific cell function, but the epigenome encodes critical information that integrates experience and cellular history for specific cell functions as well. Thus, epigenetic mechanisms provide a unique mechanism of gene expression regulation for memory processes. This may be why critical negative regulators of gene expression, such as histone deacetylases (HDACs), have powerful effects on the formation and persistence of memory. For example, HDAC inhibition has been shown to transform a subthreshold learning event into robust long-term memory and also generate a form of long-term memory that persists beyond the point at which normal long-term memory fails. A key question that is explored in this review, from a learning and memory perspective, is whether stress-dependent signaling drives the formation and persistence of long-term memory via HDAC-dependent mechanisms. PMID:24149059

Laboratory and Cloud Chamber Studies of Formation Processes and Properties of Atmospheric Ice Particles

NASA Astrophysics Data System (ADS)

Leisner, T.; Abdelmonem, A.; Benz, S.; Brinkmann, M.; Möhler, O.; Rzesanke, D.; Saathoff, H.; Schnaiter, M.; Wagner, R.

2009-04-01

The formation of ice in tropospheric clouds controls the evolution of precipitation and thereby influences climate and weather via a complex network of dynamical and microphysical processes. At higher altitudes, ice particles in cirrus clouds or contrails modify the radiative energy budget by direct interaction with the shortwave and longwave radiation. In order to improve the parameterisation of the complex microphysical and dynamical processes leading to and controlling the evolution of tropospheric ice, laboratory experiments are performed at the IMK Karlsruhe both on a single particle level and in the aerosol and cloud chamber AIDA. Single particle experiments in electrodynamic levitation lend themselves to the study of the interaction between cloud droplets and aerosol particles under extremely well characterized and static conditions in order to obtain microphysical parameters as freezing nucleation rates for homogeneous and heterogeneous ice formation. They also allow the observation of the freezing dynamics and of secondary ice formation and multiplication processes under controlled conditions and with very high spatial and temporal resolution. The inherent droplet charge in these experiments can be varied over a wide range in order to assess the influence of the electrical state of the cloud on its microphysics. In the AIDA chamber on the other hand, these processes are observable under the realistic dynamic conditions of an expanding and cooling cloud- parcel with interacting particles and are probed simultaneously by a comprehensive set of analytical instruments. By this means, microphysical processes can be studied in their complex interplay with dynamical processes as for example coagulation or particle evaporation and growth via the Bergeron - Findeisen process. Shortwave scattering and longwave absorption properties of the nucleating and growing ice crystals are probed by in situ polarised laser light scattering measurements and infrared extinction spectroscopy. In conjunction with ex situ single particle imaging and light scattering measurements the relation between the overall extinction and depolarization properties of the ice clouds and the morphological details of the constituent ice crystals are investigated. In our contribution we will concentrate on the parameterization of homogeneous and heterogeneous ice formation processes under various atmospheric conditions and on the optical properties of the ice crystals produced under these conditions. First attempts to parameterize the observations will be presented.

Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.

PubMed

Iino, Ryota; Sakakihara, Shouichi; Matsumoto, Yoshimi; Nishino, Kunihiko

2016-01-01

A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.

SigB is a dominant regulator of virulence in Staphylococcus aureus small-colony variants.

PubMed

Mitchell, Gabriel; Fugère, Alexandre; Pépin Gaudreau, Karine; Brouillette, Eric; Frost, Eric H; Cantin, André M; Malouin, François

2013-01-01

Staphylococcus aureus small-colony variants (SCVs) are persistent pathogenic bacteria characterized by slow growth and, for many of these strains, an increased ability to form biofilms and to persist within host cells. The virulence-associated gene expression profile of SCVs clearly differs from that of prototypical strains and is often influenced by SigB rather than by the agr system. One objective of this work was to confirm the role of SigB in the control of the expression of virulence factors involved in biofilm formation and intracellular persistence of SCVs. This study shows that extracellular proteins are involved in the formation of biofilm by three SCV strains, which, additionally, have a low biofilm-dispersing activity. It was determined that SigB activity modulates biofilm formation by strain SCV CF07-S and is dominant over that of the agr system without being solely responsible for the repression of proteolytic activity. On the other hand, the expression of fnbA and the control of nuclease activity contributed to the SigB-dependent formation of biofilm of this SCV strain. SigB was also required for the replication of CF07-S within epithelial cells and may be involved in the colonization of lungs by SCVs in a mouse infection model. This study methodically investigated SigB activity and associated mechanisms in the various aspects of SCV pathogenesis. Results confirm that SigB activity importantly influences the production of virulence factors, biofilm formation and intracellular persistence for some clinical SCV strains.

Two regulatory RNA elements affect TisB-dependent depolarization and persister formation.

PubMed

Berghoff, Bork A; Hoekzema, Mirthe; Aulbach, Lena; Wagner, E Gerhart H

2017-03-01

Bacterial survival strategies involve phenotypic diversity which is generated by regulatory factors and noisy expression of effector proteins. The question of how bacteria exploit regulatory RNAs to make decisions between phenotypes is central to a general understanding of these universal regulators. We investigated the TisB/IstR-1 toxin-antitoxin system of Escherichia coli to appreciate the role of the RNA antitoxin IstR-1 in TisB-dependent depolarization of the inner membrane and persister formation. Persisters are phenotypic variants that have become transiently drug-tolerant by arresting growth. The RNA antitoxin IstR-1 sets a threshold for TisB-dependent depolarization under DNA-damaging conditions, resulting in two sub-populations: polarized and depolarized cells. Furthermore, our data indicate that an inhibitory 5' UTR structure in the tisB mRNA serves as a regulatory RNA element that delays TisB translation to avoid inappropriate depolarization when DNA damage is low. Investigation of the persister sub-population further revealed that both regulatory RNA elements affect persister levels as well as persistence time. This work provides an intriguing example of how bacteria exploit regulatory RNAs to control phenotypic heterogeneity. © 2016 John Wiley & Sons Ltd.

Community-acquired methicillin-resistant Staphylococcus aureus can persist in the throat.

PubMed

Hamdan-Partida, Aida; González-García, Samuel; de la Rosa García, Estela; Bustos-Martínez, Jaime

2018-06-01

Colonization by Staphylococcus aureus is an important factor in infections caused by this microorganism. Among the colonization niches of staphylococci are the nose, skin, intestinal tract, and, recently, the throat has been given relevance. Infections caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) can be fatal. Persistence of S. aureus is an important process in the pathogenesis of this microorganism and must be studied. The aim of this study was to determine the persistence of S. aureus in the throat, and characterized the strains. We studied the persistence of S. aureus for 6 years in the throat of apparently healthy people. The isolated strains from the persistent carriers were characterized through PFGE, spa-typing, SCCmec typing, resistance to methicillin, presence of virulence genes (adhesins and toxins), and the formation of biofilm. We found persistent and intermittent carriers of S. aureus in the throat, with methicillin-sensitive (MSSA), methicillin-resistant (MRSA) strains, and confirmed for the first time that CA-MRSA colonizes this niche. These strains can colonize persistently the throat for four years or more. Typification of strains through PFGE and spa-typing revealed that some carriers present the same strain, whereas others present different strains along the period of persistence. Almost all strains induced a strong biofilm formation. All strains presented adhesin and toxin genes, but no shared genotype was found. We conclude that S. aureus, including CA-MRSA strains, can remain persistently in the throat, finding a wide variability among the persistent strains. Copyright © 2018 Elsevier GmbH. All rights reserved.

Mycobacterium tuberculosis PhoY Proteins Promote Persister Formation by Mediating Pst/SenX3-RegX3 Phosphate Sensing.

PubMed

Namugenyi, Sarah B; Aagesen, Alisha M; Elliott, Sarah R; Tischler, Anna D

2017-07-11

The Mycobacterium tuberculosis phosphate-specific transport (Pst) system controls gene expression in response to phosphate availability by inhibiting the activation of the SenX3-RegX3 two-component system under phosphate-rich conditions, but the mechanism of communication between these systems is unknown. In Escherichia coli , inhibition of the two-component system PhoR-PhoB under phosphate-rich conditions requires both the Pst system and PhoU, a putative adaptor protein. E. coli PhoU is also involved in the formation of persisters, a subpopulation of phenotypically antibiotic-tolerant bacteria. M. tuberculosis encodes two PhoU orthologs, PhoY1 and PhoY2. We generated phoY single- and double-deletion mutants and examined the expression of RegX3-regulated genes by quantitative reverse transcription-PCR (qRT-PCR). Gene expression was increased only in the Δ phoY1 Δ phoY2 double mutant and could be restored to the wild-type level by complementation with either phoY1 or phoY2 or by deletion of regX3 These data suggest that the PhoY proteins function redundantly to inhibit SenX3-RegX3 activation. We analyzed the frequencies of antibiotic-tolerant persister variants in the phoY mutants using several antibiotic combinations. Persister frequency was decreased at least 40-fold in the Δ phoY1 Δ phoY2 mutant compared to the frequency in the wild type, and this phenotype was RegX3 dependent. A Δ pstA1 mutant lacking a Pst system transmembrane component exhibited a similar RegX3-dependent decrease in persister frequency. In aerosol-infected mice, the Δ phoY1 Δ phoY2 and Δ pstA1 mutants were more susceptible to treatment with rifampin but not isoniazid. Our data demonstrate that disrupting phosphate sensing mediated by the PhoY proteins and the Pst system enhances the susceptibility of M. tuberculosis to antibiotics both in vitro and during infection. IMPORTANCE Persister variants, subpopulations of bacteria that are phenotypically antibiotic tolerant, contribute to the lengthy treatment times required to cure Mycobacterium tuberculosis infection, but the molecular mechanisms governing their formation and maintenance are poorly characterized. Here, we demonstrate that a phosphate-sensing signal transduction system, comprising the Pst phosphate transporter, the two-component system SenX3-RegX3, and functionally redundant PhoY proteins that mediate signaling between Pst and SenX3-RegX3, influences persister formation. Activation of RegX3 by deletion of the phoY genes or a Pst system component resulted in decreased persister formation in vitro Activated RegX3 also limited persister formation during growth under phosphate-limiting conditions. Importantly, increased susceptibility to the front-line drug rifampin was also observed in a mouse infection model. Thus, the M. tuberculosis phosphate-sensing signal transduction system contributes to antibiotic tolerance and is a potential target for the development of novel therapeutics that may shorten the duration of tuberculosis treatment. Copyright © 2017 Namugenyi et al.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Conlon, Brian P.; Nakayasu, Ernesto S.; Fleck, Laura E.

The current antibiotic crisis stems from two distinct phenomena-drug resistance, and drug tolerance. Resistance mechanisms such as drug efflux or modification prevent antibiotics from binding to their targets 1, allowing pathogens to grow. Antibiotic tolerance is the property of persister cells, phenotypic variants of regular bacteria 2. Antibiotics kill by corrupting targets, but these are inactive in dormant persisters, leading to tolerance. Persisters were first identified by Joseph Bigger in 1944, when he discovered a surviving sub-population of Staphylococcus following treatment with penicillin3. Persisters are largely responsible for recalcitrance of chronic diseases such as tuberculosis, and various infections associated withmore » biofilms - endocarditis, osteomyelitis, infections of catheters and indwelling devices, and deep-seated infections of soft tissues 4. There are a number of redundant pathways involved in persister formation5,6 precluding development of drugs inhibiting their formation. The acyldepsipeptide antibiotic (ADEP 4) has been shown to activate the ClpP protease resulting in death of growing cells 7. Here we show that ADEP4 activated ClpP becomes a fairly non-specific protease and kills persister cells by degradation of over 400 intracellular targets. clpP mutants are resistant to ADEP4 7, but we find that they display increased susceptibility to killing by a range of conventional antibiotics. Combining ADEP4 with rifampicin leads to eradication of persisters, stationary and biofilm populations of Staphylococcus aureus in vitro and in a deep-seated murine infection. Target corruption/activation provides an approach to killing persisters and eradicating chronic infections.« less

Bilateral persistent sciatic arteries complicated with chronic lower limb ischemia

PubMed Central

Wang, Bin; Liu, Zhenjie; Shen, Laigen

2011-01-01

INTRODUCTION Persistent sciatic artery (PSA) is a rare vascular anomaly associated with a higher rate of aneurysm formation or thromboembolic complications causing lower extremity ischemia. PRESENTATION Of Case A 15-year-old female patient with bilateral PSA presented with lower extremity ischemia. Considering the age and symptoms of the patient, we did not perform any intervention, but continued surveillance with duplex ultrasonography in case of the high incidence of aneurysmal formation or thromboembolic event. DISCUSSION Epidemiology, development, anatomical structure, diagnosis and treatments of PSAs are discussed. CONCLUSION PSAs, are prone to early atheromatous degeneration and aneurysm formation. Treatment of a PSA mainly dependent on the symptoms is either by surgical procedures or by endovascular interventions. PMID:22096762

Modelling biofilm-induced formation damage and biocide treatment in subsurface geosystems

PubMed Central

Ezeuko, C C; Sen, A; Gates, I D

2013-01-01

Biofilm growth in subsurface porous media, and its treatment with biocides (antimicrobial agents), involves a complex interaction of biogeochemical processes which provide non-trivial mathematical modelling challenges. Although there are literature reports of mathematical models to evaluate biofilm tolerance to biocides, none of these models have investigated biocide treatment of biofilms growing in interconnected porous media with flow. In this paper, we present a numerical investigation using a pore network model of biofilm growth, formation damage and biocide treatment. The model includes three phases (aqueous, adsorbed biofilm, and solid matrix), a single growth-limiting nutrient and a single biocide dissolved in the water. Biofilm is assumed to contain a single species of microbe, in which each cell can be a viable persister, a viable non-persister, or non-viable (dead). Persisters describe small subpopulation of cells which are tolerant to biocide treatment. Biofilm tolerance to biocide treatment is regulated by persister cells and includes ‘innate’ and ‘biocide-induced’ factors. Simulations demonstrate that biofilm tolerance to biocides can increase with biofilm maturity, and that biocide treatment alone does not reverse biofilm-induced formation damage. Also, a successful application of biological permeability conformance treatment involving geologic layers with flow communication is more complicated than simply engineering the attachment of biofilm-forming cells at desired sites. PMID:23164434

Medial prefrontal cortex dopamine controls the persistent storage of aversive memories

PubMed Central

Gonzalez, María C.; Kramar, Cecilia P.; Tomaiuolo, Micol; Katche, Cynthia; Weisstaub, Noelia; Cammarota, Martín; Medina, Jorge H.

2014-01-01

Medial prefrontal cortex (mPFC) is essential for initial memory processing and expression but its involvement in persistent memory storage has seldom been studied. Using the hippocampus dependent inhibitory avoidance learning task and the hippocampus-independent conditioned taste aversion paradigm together with specific dopamine receptor agonists and antagonists we found that persistence but not formation of long-term aversive memories requires dopamine D1/D5 receptors activation in mPFC immediately after training and, depending on the task, between 6 and 12 h later. Our results indicate that besides its well-known participation in retrieval and early consolidation, mPFC also modulates the endurance of long-lasting aversive memories regardless of whether formation of the aversive mnemonic trace requires the participation of the hippocampus. PMID:25506318

Molecular brake pad hypothesis: pulling off the brakes for emotional memory

PubMed Central

Vogel-Ciernia, Annie

2015-01-01

Under basal conditions histone deacetylases (HDACs) and their associated co-repressor complexes serve as molecular ‘brake pads’ to prevent the gene expression required for long-term memory formation. Following a learning event, HDACs and their co-repressor complexes are removed from a subset of specific gene promoters, allowing the histone acetylation and active gene expression required for long-term memory formation. Inhibition of HDACs increases histone acetylation, extends gene expression profiles, and allows for the formation of persistent long-term memories for training events that are otherwise forgotten. We propose that emotionally salient experiences have utilized this system to form strong and persistent memories for behaviorally significant events. Consequently, the presence or absence of HDACs at a selection of specific gene promoters could serve as a critical barrier for permitting the formation of long-term memories. PMID:23096102

Common Methodology for Efficient Airspace Operations

NASA Technical Reports Server (NTRS)

Sridhar, Banavar

2012-01-01

Topics include: a) Developing a common methodology to model and avoid disturbances affecting airspace. b) Integrated contrails and emission models to a national level airspace simulation. c) Developed capability to visualize, evaluate technology and alternate operational concepts and provide inputs for policy-analysis tools to reduce the impact of aviation on the environment. d) Collaborating with Volpe Research Center, NOAA and DLR to leverage expertise and tools in aircraft emissions and weather/climate modeling. Airspace operations is a trade-off balancing safety, capacity, efficiency and environmental considerations. Ideal flight: Unimpeded wind optimal route with optimal climb and descent. Operations degraded due to reduction in airport and airspace capacity caused by inefficient procedures and disturbances.

Persistent hyperplastic tunica vasculosa lentis and persistent hyperplastic primary vitreous in transgenic line TgN3261Rpw.

PubMed

Colitz, C M; Malarkey, D E; Woychik, R P; Wilkinson, J E

2000-09-01

Persistent hyperplastic tunica vasculosa lentis and persistent hyperplastic primary vitreous are congenital ocular anomalies that can lead to cataract formation. A line of insertional mutant mice, TgN3261Rpw, generated at the Oak Ridge National Laboratory in a large-scale insertional mutagenesis program was found to have a low incidence (8/243; 3.29%) of multiple developmental ocular abnormalities. The ocular abnormalities include persistent hyperplastic primary vitreous, persistent hyperplastic tunica vasculosa lentis, failure of cleavage of the anterior segment, retrolental fibrovascular membrane, posterior polar cataract, and detached retina. This transgenic mouse line provides an ontogenetic model because of the high degree of similarity of this entity in humans, dogs, and mice.

Cortical dynamics of feature binding and reset: control of visual persistence.

PubMed

Francis, G; Grossberg, S; Mingolla, E

1994-04-01

An analysis of the reset of visual cortical circuits responsible for the binding or segmentation of visual features into coherent visual forms yields a model that explains properties of visual persistence. The reset mechanisms prevent massive smearing of visual percepts in response to rapidly moving images. The model simulates relationships among psychophysical data showing inverse relations of persistence to flash luminance and duration, greater persistence of illusory contours than real contours, a U-shaped temporal function for persistence of illusory contours, a reduction of persistence due to adaptation with a stimulus of like orientation, an increase of persistence with spatial separation of a masking stimulus. The model suggests that a combination of habituative, opponent, and endstopping mechanisms prevent smearing and limit persistence. Earlier work with the model has analyzed data about boundary formation, texture segregation, shape-from-shading, and figure-ground separation. Thus, several types of data support each model mechanism and new predictions are made.

RESEARCH ON EMISSIONS AND MITIGATION OF POP'S FROM COMBUSTION SOURCES

EPA Science Inventory

Chapter summarizes EPA's research on emissions and control of persistent organic pollutants (POPS) from combustion sources, with emphasis on source characterization and measurement, formation and destruction mechanisms, formation prevention, and flue gas cleaning. Laboratory exp...

Validation of XCO2 derived from SWIR of GOSAT TANSO-FTS with aircraft measurement data

NASA Astrophysics Data System (ADS)

Inoue, M.; Morino, I.; Uchino, O.; Miyamoto, Y.; Yoshida, Y.; Yokota, T.; Machida, T.; Sawa, Y.; Matsueda, H.; Sweeney, C.; Tans, P. P.; Andrews, A. E.; Patra, P. K.

2011-12-01

Column-averaged mixing ratios of carbon dioxide (XCO2) are retrieved from the Short-Wavelength InfraRed (SWIR) spectrum of Thermal And Near-infrared Sensor for carbon Observation - Fourier Transform Spectrometer (TANSO-FTS) onboard the Greenhouse gases Observing SATellite (GOSAT). They are compared with the aircraft data measured by the Comprehensive Observation Network for TRace gases by AIrLiner (CONTRAIL) project, National Oceanic and Atmospheric Administration (NOAA), and National Institute for Environmental Studies (NIES). Because limited aircraft measurement was conducted within a few hours of the GOSAT overpass time, we prepared temporally interpolated aircraft-based XCO2 data by fitting with the function that contains yearly trend and annual/semiannual sinusoidal variations to compare with GOSAT XCO2. As for the GOSAT XCO2 data, those retrieved within ±2 degrees or ±5 degrees latitude/longitude box centered at each observation site were used. In order to compare the aircraft data with the satellite data, the GOSAT column averaging kernels (CAK) should be taken into account to calculate the aircraft-based XCO2. However, it is hard to apply GOSAT CAK with temporally interpolated aircraft data. Therefore, we evaluated the GOSAT CAK impact on the aircraft-based XCO2 calculation by using CONTRAIL data over Narita (35.8N, 140.4E) where the maximum temporally match-upped data are available. The difference in the aircraft-based XCO2 with and without GOSAT CAK is lower by approximately 0.3 ppm. Here, we made a comparison between GOSAT XCO2 and aircraft-based XCO2 without GOSAT CAK. Although GOSAT data are underestimated by approximately 7-9 ppm for comparison in every site, there is a good correlation between both datasets in some sites, such as high-latitude regions of North America, Europe, Siberia, and ocean regions. A direct comparison between GOSAT and aircraft XCO2 at all observation sites shows that GOSAT SWIR XCO2 is biased low by about 8 ppm.

Measurements of Nucleation-Mode Particle Size Distributions in Aircraft Plumes during SULFUR 6

NASA Technical Reports Server (NTRS)

Brock, Charles A.; Bradford, Deborah G.

1999-01-01

This report summarizes the participation of the University of Denver in an airborne measurement program, SULFUR 6, which was undertaken in late September and early October of 1998 by the Deutsches Zentrum fur Luft und Raumfahrt (DLR). Scientific findings from two papers that have been published or accepted and from one manuscript that is in preparation are presented. The SULFUR 6 experiment was designed to investigate the emissions from subsonic aircraft to constrain calculations of possible atmospheric chemical and climatic effects. The University of Denver effort contributed toward the following SULFUR 6 goals: (1) To investigate the relationship between fuel sulfur content (FSC--mass of sulfur per mass of fuel) and particle number and mass emission index (El--quantity emitted per kg of fuel burned); (2) To provide upper and lower limits for the mass conversion efficiency (nu) of fuel sulfur to gaseous and particulate sulfuric acid; (3) To constrain models of volatile particle nucleation and growth by measuring the particle size distribution between 3 and 100 nm at aircraft plume ages ranging from 10(exp -1) to 10(exp 3) s; (4) To determine microphysical and optical properties and bulk chemical composition of soot particles in aircraft exhaust; and (5) To investigate the differences in particle properties between aircraft plumes in contrail and non-contrail situations. The experiment focused on emissions from the ATTAS research aircraft (a well characterized, but older technology turbojet) and from an in-service Boeing 737-300 aircraft provided by Lufthansa, with modem, high-bypass turbofan engines. Measurements were made from the DLR Dassault Falcon 900 aircraft, a modified business jet. The Atmospheric Effects of Aviation Program (AEAP) provided funding to operate an instrument, the nucleation-mode aerosol size spectrometer (N-MASS), during the SULFUR 6 campaign and to analyze the data. The N-MASS was developed at the University of Denver with the support of NOAA's Office of Global Programs and NASA's AEAP and measures particle size distributions in the 4-100 nm range.

Probabilistic description of ice-supersaturated layers in low resolution profiles of relative humidity N. C. Dickson, K. Gierens, H. L. Rogers, R. L. Jones

NASA Astrophysics Data System (ADS)

Dickson, N.

2009-12-01

The global observation, assimilation and prediction in numerical models of ice super-saturated (ISS) regions (ISSR) are crucial if the climate impact of aircraft condensations trails (contrails) is to be fully understood, and if, for example, contrail formation is to be avoided through aircraft operational measures. A robust assessment of the global distribution of ISSR will further this debate, and ISS event occurrence, frequency and spatial scales have recently attracted significant attention. The mean horizontal size of ISSR is 150 km (±250km) although 12-14% of ISS events occur on horizontal scales of less than 5km. The average vertical thickness of ISS layers is 600-800m (±575m) but layers ranging from 25m to 3000m have been observed, with up to one third of ISS layers thought to be less than 100m deep. Given their small scales compared to typical atmospheric model grid sizes, statistical representations of the spatial scales of ISSR are required, in both horizontal and vertical dimensions, if global occurrence of ISSR is to be adequately represented in climate models. This paper uses radiosonde launches made by the UK Meteorological Office, from the British Isles, Gibraltar, St. Helena and the Falkland Islands between January 2002 and December 2006, to investigate the probabilistic occurrence of ISSR. Specifically each radiosonde profile is divided into 50 and 100 hPa pressure layers, to emulate the coarse vertical resolution of some atmospheric models. Then the high resolution observations contained within each thick pressure layer are used to calculate an average relative humidity and an ISS fraction for each individual thick pressure layer. These relative humidity pressure layer descriptions are then linked through a probability function to produce an s-shaped curve describing the ISS fraction in any average relative humidity pressure layer. An empirical investigation has shown that this one curve is statistically valid for mid-latitude locations, irrespective of season and altitude, however, pressure layer depth is an important variable. Using this empirical understanding of the s-shaped relationship a mathematical model was developed to represent the ISS fraction within any arbitrary thick pressure layer. Here the statistical distributions of actual high resolution RHi observations in any thick pressure layer, along with an error function, are used to mathematically describe the s-shape. Two models were developed to represent both 50 and 100 hPa pressure layers with each reconstructing their respective s-shapes within 8-10% of the empirical curves. These new models can be used, to represent the small scale structures of ISS events, in modelled data where only low vertical resolution is available. This will be useful in understanding, and improving the global distribution, both observed and forecasted, of ice super-saturation.

Space-time crystals of trapped ions.

PubMed

Li, Tongcang; Gong, Zhe-Xuan; Yin, Zhang-Qi; Quan, H T; Yin, Xiaobo; Zhang, Peng; Duan, L-M; Zhang, Xiang

2012-10-19

Spontaneous symmetry breaking can lead to the formation of time crystals, as well as spatial crystals. Here we propose a space-time crystal of trapped ions and a method to realize it experimentally by confining ions in a ring-shaped trapping potential with a static magnetic field. The ions spontaneously form a spatial ring crystal due to Coulomb repulsion. This ion crystal can rotate persistently at the lowest quantum energy state in magnetic fields with fractional fluxes. The persistent rotation of trapped ions produces the temporal order, leading to the formation of a space-time crystal. We show that these space-time crystals are robust for direct experimental observation. We also study the effects of finite temperatures on the persistent rotation. The proposed space-time crystals of trapped ions provide a new dimension for exploring many-body physics and emerging properties of matter.

Pesticide nonextractable residue formation in soil: insights from inverse modeling of degradation time series.

PubMed

Loos, Martin; Krauss, Martin; Fenner, Kathrin

2012-09-18

Formation of soil nonextractable residues (NER) is central to the fate and persistence of pesticides. To investigate pools and extent of NER formation, an established inverse modeling approach for pesticide soil degradation time series was evaluated with a Monte Carlo Markov Chain (MCMC) sampling procedure. It was found that only half of 73 pesticide degradation time series from a homogeneous soil source allowed for well-behaved identification of kinetic parameters with a four-pool model containing a parent compound, a metabolite, a volatile, and a NER pool. A subsequent simulation indeed confirmed distinct parameter combinations of low identifiability. Taking the resulting uncertainties into account, several conclusions regarding NER formation and its impact on persistence assessment could nonetheless be drawn. First, rate constants for transformation of parent compounds to metabolites were correlated to those for transformation of parent compounds to NER, leading to degradation half-lives (DegT50) typically not being larger than disappearance half-lives (DT50) by more than a factor of 2. Second, estimated rate constants were used to evaluate NER formation over time. This showed that NER formation, particularly through the metabolite pool, may be grossly underestimated when using standard incubation periods. It further showed that amounts and uncertainties in (i) total NER, (ii) NER formed from the parent pool, and (iii) NER formed from the metabolite pool vary considerably among data sets at t→∞, with no clear dominance between (ii) and (iii). However, compounds containing aromatic amine moieties were found to form significantly more total NER when extrapolating to t→∞ than the other compounds studied. Overall, our study stresses the general need for assessing uncertainties, identifiability issues, and resulting biases when using inverse modeling of degradation time series for evaluating persistence and NER formation.

Progress of Validation of GOSAT Standard Products

NASA Astrophysics Data System (ADS)

Uchino, Osamu

2010-05-01

Isamu Morino, Tomoaki Tanaka, Yuki Miyamoto, Yukio Yoshida, Tatsuya Yokota, Toshinobu Machida National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan Debra Wunch, Paul Wennberg Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA Geoffrey Toon Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, USA Thorsten Warneke, Justus Notholt Institute of Environmental Physics, University of Bremen, Bremen, Germany David Griffith, Nicholas Deutscher Department of Chemistry, University of Wollongong, Wollongong New South Wales, Australia Vanessa Sherlock National Institute of Water and Atmospheric Research, Lauder, Central Otago, New Zealand Hidekazu Matsueda, Yousuke Sawa Meteorological Research Institute, 1-1 Nagamine, Tsukuba, Ibaraki 305-0052, Japan Colm Sweeney, Pieter Tans Earth System Research Laboratory, NOAA, Boulder, USA The Greenhouse gases Observing SATellite (GOSAT), launched on 23 January 2009, is the world's first satellite dedicated to measuring the concentrations of the two major greenhouse gases, carbon dioxide (CO2) and methane (CH4), from space. The data measured with the Thermal And Near-infrared Sensor for carbon Observation Fourier Transform Spectrometer (TANSO-FTS) and the Cloud and Aerosol Imager (TANSO-CAI) are processed into several types of data products. Column abundances of CO2 and CH4 (TANSO-FTS SWIR L2 data product) are retrieved from the FTS L1B spectral data. Validation of the FTS Level 2 data product is critical since the data is used for generating the FTS Level 3 (global distributions of column-averaged mixing ratio data of XCO2 and XCH4) and the FTS Level 4 (regional CO2 fluxes and three dimensional distribution of CO2 calculated from the estimated fluxes) products. The reference data to be used for validating abundances are required to have uncertainties of less than 1.0 % (0.3 % or 1 ppm is desirable) for CO2 and 2.0 % for CH4. Ground-based high-resolution FTSs that measure direct solar light are known to have the highest precision in observing column abundances of CO2 and CH4. Data provided from TCCON (Total Carbon Column Observing Network) have been used for the GOSAT data validation. The major error factors in the retrieval of the Level 2 column abundances of CO2 and CH4 are interferences by aerosols and thin cirrus clouds. To elucidate their influences on the column abundance retrieval, we measure aerosols and cirrus clouds using lidars and/or sky-radiometers at selected FTS sites. Concentrations of CO2 and CH4 measured by CONTRAIL (Comprehensive Observation Network for Trace gases by AIrLiner) are also of great importance in validating the Level 2 data product. In the CONTRAIL project, vertical profiles of CO2 concentrations are obtained during the take-off and landing periods at uncertainties of 0.2 ppm. These profiles are used to calculate XCO2. Furthermore airborne data prepared by NOAA and NIES are utilized in the validation work. We will present recent results of the validation activity in which we compare the Level 2 column concentrations against the reference data provided from TCCON, CONTRAIL, NOAA, and NIES.

Reassessing the Role of Type II Toxin-Antitoxin Systems in Formation of Escherichia coli Type II Persister Cells.

PubMed

Goormaghtigh, Frédéric; Fraikin, Nathan; Putrinš, Marta; Hallaert, Thibaut; Hauryliuk, Vasili; Garcia-Pino, Abel; Sjödin, Andreas; Kasvandik, Sergo; Udekwu, Klas; Tenson, Tanel; Kaldalu, Niilo; Van Melderen, Laurence

2018-06-12

Persistence is a reversible and low-frequency phenomenon allowing a subpopulation of a clonal bacterial population to survive antibiotic treatments. Upon removal of the antibiotic, persister cells resume growth and give rise to viable progeny. Type II toxin-antitoxin (TA) systems were assumed to play a key role in the formation of persister cells in Escherichia coli based on the observation that successive deletions of TA systems decreased persistence frequency. In addition, the model proposed that stochastic fluctuations of (p)ppGpp levels are the basis for triggering activation of TA systems. Cells in which TA systems are activated are thought to enter a dormancy state and therefore survive the antibiotic treatment. Using independently constructed strains and newly designed fluorescent reporters, we reassessed the roles of TA modules in persistence both at the population and single-cell levels. Our data confirm that the deletion of 10 TA systems does not affect persistence to ofloxacin or ampicillin. Moreover, microfluidic experiments performed with a strain reporting the induction of the yefM-yoeB TA system allowed the observation of a small number of type II persister cells that resume growth after removal of ampicillin. However, we were unable to establish a correlation between high fluorescence and persistence, since the fluorescence of persister cells was comparable to that of the bulk of the population and none of the cells showing high fluorescence were able to resume growth upon removal of the antibiotic. Altogether, these data show that there is no direct link between induction of TA systems and persistence to antibiotics. IMPORTANCE Within a growing bacterial population, a small subpopulation of cells is able to survive antibiotic treatment by entering a transient state of dormancy referred to as persistence. Persistence is thought to be the cause of relapsing bacterial infections and is a major public health concern. Type II toxin-antitoxin systems are small modules composed of a toxic protein and an antitoxin protein counteracting the toxin activity. These systems were thought to be pivotal players in persistence until recent developments in the field. Our results demonstrate that previous influential reports had technical flaws and that there is no direct link between induction of TA systems and persistence to antibiotics. Copyright © 2018 Goormaghtigh et al.

Differences in biofilm formation of produce and poultry Salmonella enterica isolates and their persistence on spinach plants

USDA-ARS?s Scientific Manuscript database

Repeat irrigation of spinach plants with water containing Salmonella was used to determine Salmonella persistence on spinach leaves. Spinach plants were irrigated four times (biweekly) with water containing ca. 2.1 log CFU Salmonella per 100 ml water (the maximum generic E. coli MPN recommended by...

Effects of a Strengths-Based First-Year Seminar on Student Thriving

ERIC Educational Resources Information Center

Shelburne, Nathan Andrew

2016-01-01

Colleges and universities commonly implement first-year seminars to support new students during the challenging and formative first semester. These programs are widely regarded as highly effective in promoting student persistence through the first year and beyond. However, attention on the indirect outcome of persistence as the primary measure of…

Director's Discretionary Fund Report for Fiscal Year 1996

NASA Technical Reports Server (NTRS)

1997-01-01

Topics covered include: Waterproofing the Space Shuttle tiles, thermal protection system for Reusable Launch Vehicles, computer modeling of the thermal conductivity of cometary ice, effects of ozone depletion and ultraviolet radiation on plants, a novel telemetric biosensor to monitor blood pH on-line, ion mobility in polymer electrolytes for lithium-polymer batteries, a microwave-pumped far infrared photoconductor, and a new method for measuring cloud liquid vapor using near infrared remote sensing. Also included: laser-spectroscopic instrument for turbulence measurement, remote sensing of aircraft contrails using a field portable imaging interferometer, development of a silicon-micromachined gas chromatography system for determination of planetary surface composition, planar Doppler velocimetry, chaos in interstellar chemistry, and a limited pressure cycle engine for high-speed output.

A search for life on earth at 100 meter resolution

NASA Technical Reports Server (NTRS)

Sagan, C.; Wallace, D.

1970-01-01

A study of several thousand photos indicated approximately 1% of Gemini and Apollo photographs of the earth at 100 m resolution revealed signs of life; rectangular arrays due to human agricultural and urban territoriality, roads, canals, jet contrails, and industrial pollution. Potential false positives such as dunes, sand bars, and jet stream clouds abound. A curve was derived for the detectivity of contemporary life on earth, in a plot of ground resolution versus global coverage. A comparable biology on Mars would not have been detected by all observations of Mars through Mariner 7. Forthcoming Mars orbiter and lander imaging experiments hold significant promise of detecting life on Mars of contemporary terrestrial extent and advancement, should such life exist.

Phenotypic and genetic heterogeneity within biofilms with particular emphasis on persistence and antimicrobial tolerance.

PubMed

Sadiq, Faizan A; Flint, Steve; Li, YanJun; Ou, Kai; Yuan, Lei; He, Guo Qing

2017-09-01

Phenotypic changes or phase variation within biofilms is an important feature of bacterial dormant life. Enhanced resistance to antimicrobials is one of the distinct features displayed by a fraction of cells within biofilms. It is believed that persisters are mainly responsible for this phenotypic heterogeneity. However, there is still an unresolved debate on the formation of persisters. In this short review, we highlight all known genomic and proteomic changes encountered by bacterial cells within biofilms. We have also described all phenotypic changes displayed by bacterial cells within biofilms with particular emphasis on enhanced antimicrobial tolerance of biofilms with particular reference to persisters. In addition, all currently known models of persistence have been succinctly discussed.

Factors Mediating Environmental Biofilm Formation by Legionella pneumophila.

PubMed

Abu Khweek, Arwa; Amer, Amal O

2018-01-01

Legionella pneumophila ( L. pneumophila ) is an opportunistic waterborne pathogen and the causative agent for Legionnaires' disease, which is transmitted to humans via inhalation of contaminated water droplets. The bacterium is able to colonize a variety of man-made water systems such as cooling towers, spas, and dental lines and is widely distributed in multiple niches, including several species of protozoa In addition to survival in planktonic phase, L. pneumophila is able to survive and persist within multi-species biofilms that cover surfaces within water systems. Biofilm formation by L. pneumophila is advantageous for the pathogen as it leads to persistence, spread, resistance to treatments and an increase in virulence of this bacterium. Furthermore, Legionellosis outbreaks have been associated with the presence of L. pneumophila in biofilms, even after the extensive chemical and physical treatments. In the microbial consortium-containing L. pneumophila among other organisms, several factors either positively or negatively regulate the presence and persistence of L. pneumophila in this bacterial community. Biofilm-forming L. pneumophila is of a major importance to public health and have impact on the medical and industrial sectors. Indeed, prevention and removal protocols of L. pneumophila as well as diagnosis and hospitalization of patients infected with this bacteria cost governments billions of dollars. Therefore, understanding the biological and environmental factors that contribute to persistence and physiological adaptation in biofilms can be detrimental to eradicate and prevent the transmission of L. pneumophila . In this review, we focus on various factors that contribute to persistence of L. pneumophila within the biofilm consortium, the advantages that the bacteria gain from surviving in biofilms, genes and gene regulation during biofilm formation and finally challenges related to biofilm resistance to biocides and anti-Legionella treatments.

Biofilm Formation on Stainless Steel by Streptococcus thermophilus UC8547 in Milk Environments Is Mediated by the Proteinase PrtS.

PubMed

Bassi, D; Cappa, F; Gazzola, S; Orrù, L; Cocconcelli, P S

2017-04-15

In Streptococcus thermophilus , gene transfer events and loss of ancestral traits over the years contribute to its high level of adaptation to milk environments. Biofilm formation capacity, a phenotype that is lost in the majority of strains, plays a role in persistence in dairy environments, such as milk pasteurization and cheese manufacturing plants. To investigate this property, we have studied S. thermophilus UC8547, a fast-acidifying dairy starter culture selected for its high capacity to form biofilm on stainless steel under environmental conditions resembling the dairy environment. Using a dynamic flow cell apparatus, it was shown that S. thermophilus UC8547 biofilm formation on stainless steel depends on the presence of milk proteins. From this strain, which harbors the prtS gene for the cell wall protease and shows an aggregative phenotype, spontaneous mutants with impaired biofilm capacity can be isolated at high frequency. These mutants lack the PrtS expendable island, as confirmed by comparison of the genome sequence of UC8547Δ3 with that of the parent strain. The prtS island excision occurs between two 26-bp direct repeats located in the two copies of the IS Sth1 flanking this genomic island. The central role of PrtS was confirmed by analyzing the derivative strain UC8547Δ16, whose prtS gene was interrupted by an insertional mutation, thereby making it incapable of biofilm formation. PrtS, acting as a binding substance between the milk proteins adhered to stainless steel and S. thermophilus cell envelopes, mediates biofilm formation in dairy environments. This feature provides S. thermophilus with an ecological benefit for its survival and persistence in this environment. IMPORTANCE The increased persistence of S. thermophilus biofilm has consequences in the dairy environment: if, on the one hand, the release of this microorganism from biofilm can promote the fermentation of artisanal cheeses, under industrial conditions it may lead to undesirable contamination of dairy products. The study of the molecular mechanism driving S. thermophilus biofilm formation provides increased knowledge on how an ancestral trait affects relevant phenotypes, such as persistence in the environment and efficiency of growth in milk. This study provides insight into the genetic factors affecting biofilm formation at dairy plants. Copyright © 2017 American Society for Microbiology.

Biofilm Formation on Stainless Steel by Streptococcus thermophilus UC8547 in Milk Environments Is Mediated by the Proteinase PrtS

PubMed Central

Cappa, F.; Gazzola, S.; Orrù, L.

2017-01-01

ABSTRACT In Streptococcus thermophilus, gene transfer events and loss of ancestral traits over the years contribute to its high level of adaptation to milk environments. Biofilm formation capacity, a phenotype that is lost in the majority of strains, plays a role in persistence in dairy environments, such as milk pasteurization and cheese manufacturing plants. To investigate this property, we have studied S. thermophilus UC8547, a fast-acidifying dairy starter culture selected for its high capacity to form biofilm on stainless steel under environmental conditions resembling the dairy environment. Using a dynamic flow cell apparatus, it was shown that S. thermophilus UC8547 biofilm formation on stainless steel depends on the presence of milk proteins. From this strain, which harbors the prtS gene for the cell wall protease and shows an aggregative phenotype, spontaneous mutants with impaired biofilm capacity can be isolated at high frequency. These mutants lack the PrtS expendable island, as confirmed by comparison of the genome sequence of UC8547Δ3 with that of the parent strain. The prtS island excision occurs between two 26-bp direct repeats located in the two copies of the ISSth1 flanking this genomic island. The central role of PrtS was confirmed by analyzing the derivative strain UC8547Δ16, whose prtS gene was interrupted by an insertional mutation, thereby making it incapable of biofilm formation. PrtS, acting as a binding substance between the milk proteins adhered to stainless steel and S. thermophilus cell envelopes, mediates biofilm formation in dairy environments. This feature provides S. thermophilus with an ecological benefit for its survival and persistence in this environment. IMPORTANCE The increased persistence of S. thermophilus biofilm has consequences in the dairy environment: if, on the one hand, the release of this microorganism from biofilm can promote the fermentation of artisanal cheeses, under industrial conditions it may lead to undesirable contamination of dairy products. The study of the molecular mechanism driving S. thermophilus biofilm formation provides increased knowledge on how an ancestral trait affects relevant phenotypes, such as persistence in the environment and efficiency of growth in milk. This study provides insight into the genetic factors affecting biofilm formation at dairy plants. PMID:28159787

Executive Functions and Prefrontal Cortex: A Matter of Persistence?

PubMed Central

Ball, Gareth; Stokes, Paul R.; Rhodes, Rebecca A.; Bose, Subrata K.; Rezek, Iead; Wink, Alle-Meije; Lord, Louis-David; Mehta, Mitul A.; Grasby, Paul M.; Turkheimer, Federico E.

2011-01-01

Executive function is thought to originates from the dynamics of frontal cortical networks. We examined the dynamic properties of the blood oxygen level dependent time-series measured with functional MRI (fMRI) within the prefrontal cortex (PFC) to test the hypothesis that temporally persistent neural activity underlies performance in three tasks of executive function. A numerical estimate of signal persistence, the Hurst exponent, postulated to represent the coherent firing of cortical networks, was determined and correlated with task performance. Increasing persistence in the lateral PFC was shown to correlate with improved performance during an n-back task. Conversely, we observed a correlation between persistence and increasing commission error – indicating a failure to inhibit a prepotent response – during a Go/No-Go task. We propose that persistence within the PFC reflects dynamic network formation and these findings underline the importance of frequency analysis of fMRI time-series in the study of executive functions. PMID:21286223

Characterization of multi-drug tolerant persister cells in Streptococcus suis.

PubMed

Willenborg, Jörg; Willms, Daniela; Bertram, Ralph; Goethe, Ralph; Valentin-Weigand, Peter

2014-05-12

Persister cells constitute a subpopulation of dormant cells within a microbial population which are genetically identical but phenotypically different to regular cells. Notably, persister cells show an elevated tolerance to antimicrobial agents. Thus, they are considered to represent a microbial 'bet-hedging' strategy and are of particular importance in pathogenic bacteria. We studied the ability of the zoonotic pathogen Streptococcus (S.) suis to form multi-drug tolerant variants and identified persister cells dependent on the initial bacterial growth phase. We observed lower numbers of persisters in exponential phase cultures than in stationary growth phase populations. S. suis persister cells showed a high tolerance to a variety of antibiotics, and the phenotype was not inherited as tested with four passages of S. suis populations. Furthermore, we provide evidence that the persister phenotype is related to expression of genes involved in general metabolic pathways since we found higher numbers of persister cells in a mutant strain defective in the catabolic arginine deiminase system as compared to its parental wild type strain. Finally, we observed persister cell formation also in other S. suis strains and pathogenic streptococcal species. Taken together, this is the first study that reports multi-drug tolerant persister cells in the zoonotic pathogen S. suis.

Distributed metadata servers for cluster file systems using shared low latency persistent key-value metadata store

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bent, John M.; Faibish, Sorin; Pedone, Jr., James M.

A cluster file system is provided having a plurality of distributed metadata servers with shared access to one or more shared low latency persistent key-value metadata stores. A metadata server comprises an abstract storage interface comprising a software interface module that communicates with at least one shared persistent key-value metadata store providing a key-value interface for persistent storage of key-value metadata. The software interface module provides the key-value metadata to the at least one shared persistent key-value metadata store in a key-value format. The shared persistent key-value metadata store is accessed by a plurality of metadata servers. A metadata requestmore » can be processed by a given metadata server independently of other metadata servers in the cluster file system. A distributed metadata storage environment is also disclosed that comprises a plurality of metadata servers having an abstract storage interface to at least one shared persistent key-value metadata store.« less

Space-Time Crystals of Trapped Ions

DTIC Science & Technology

2012-10-15

Spontaneous symmetry breaking can lead to the formation of time crystals, as well as spatial crystals. Here we propose a space- time crystal of...fields with fractional fluxes. The persistent rotation of trapped ions produces the temporal order, leading to the formation of a space- time crystal . We

METABOLISM OF BENZO[A]PYRENE AND PERSISTENCE OF DNA ADDUCTS IN THE BROWN BULLHEAD (ICTALURUS NEBULOSUS)

EPA Science Inventory

The in vitro metabolism of [3H]benzo[a]pyrene (BP) and [14C]benzo[a]pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus Nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. ompared to rat liver microsomes, bullhead l...

Validation of AIRS Retrievals of CO2 via Comparison to In Situ Measurements

NASA Technical Reports Server (NTRS)

Olsen, Edward T.; Chahine, Moustafa T.; Chen, Luke L.; Jiang, Xun; Pagano, Thomas S.; Yung, Yuk L.

2008-01-01

Topics include AIRS on Aqua, 2002-present with discussion about continued operation to 2011 and beyond and background, including spectrum, weighting functions, and initialization; comparison with aircraft and FTIR measurements in Masueda (CONTRAIL) JAL flask measurements, Park Falls, WI FTIR, Bremen, GDF, and Spitsbergen, Norway; AIRS retrievals over addition FTIR sites in Darwin, AU and Lauder, NZ; and mid-tropospheric carbon dioxide weather and contribution from major surface sources. Slide titles include typical AIRS infrared spectrum, AIRS sensitivity for retrieving CO2 profiles, independence of CO2 solution with respect to the initial guess, available in situ measurements for validation and comparison, comparison of collocated V1.5x AIRS CO2 (N_coll greater than or equal to 9) with INTEX-NA and SPURT;

Plume and wake dynamics, mixing, and chemistry behind an HSCT aircraft

NASA Technical Reports Server (NTRS)

Miake-Lye, R. C.; Martinez-Sanchez, M.; Brown, R. C.; Kolb, C. E.

1991-01-01

The chemical evolution and mixing and vortical motion of a High Speed Civil Transport's engine exhausts must be analyzed in order to track the gas and its speciation as emissions are mixed to atmospheric scales. Attention is presently given to an analytic model of the wake dynamical processes which accounts for the roll-up of the trailing vorticity, its breakup due to the Crow instability, and the subsequent evolution and motion of the reconnected vorticity. The concentrated vorticity is noted to wrap up the buoyant exhaust and suppress its continued mixing and dilution. The species tracked encompass those which could be heterogeneously reactive on the surfaces of the condensed ice particles, and those capable of reacting with exhaust soot particle surfaces to form active contrail and/or cloud condensation nuclei.

KSC-2011-5843

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

KSC-2011-5847

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it touches down on Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

KSC-2011-5841

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

KSC-2011-5845

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

KSC-2011-5846

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it touches down on Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

KSC-2011-5844

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

KSC-2011-5842

NASA Image and Video Library

2011-07-21

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Atlantis as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Atlantis marked the 26th nighttime landing of NASA's Space Shuttle Program and the 78th landing at Kennedy. It also was the final mission for the shuttle program. STS-135 was the 33rd and final flight for Atlantis, which has spent 307 days in space, orbited Earth 4,848 times and traveled 125,935,769 miles. For more information on the space shuttle era, visit www.nasa.gov/mission_pages/shuttle/flyout. Photo credit: NASA/Sandra Joseph and Kevin O'Connell

Stellar Contrails in Quasi-stellar Objects: The Origin of Broad Absorption Lines

NASA Astrophysics Data System (ADS)

Scoville, Nick; Norman, Colin

1995-10-01

Active galactic nuclei (AGNs) and quasars often exhibit infrared excesses at λ = 2-10 microns attributable to thermal dust emission. In this paper we propose that this hot dust is supplied by circumstellar mass loss from evolved stars in the nuclear star cluster. The physics of the mass-loss dust, specifically the evaporation temperature, is a critical parameter in determining the accretion rate of mass-loss material onto the central AGN. For standard interstellar dust grains with an evaporation temperature of 1800 K the dust is destroyed inside a radius of 1 pc from a central luminosity source of 5 × 10 Lsun. The mass-loss material inside 1 pc will therefore have a lower radiation pressure efficiency and accrete inward. Outside this critical radius, dust may survive, and the mass loss is accelerated outward owing to the high radiation pressure efficiency of the dust mixed with the gas. The outflowing material will consist of discrete trails of debris shed by the individual mass-loss stars, and we suggest that these trails produce the broad absorption lines (BALs) seen in 5%-10% of QSOs. The model accounts naturally for the maximum outflow velocities seen in the BALs (˜30,000 km s-1 and varying as L¼) since this maximum terminal velocity occurs for matter originating at the inner edge of the radiative equilibrium dust survival zone. Although the radiation pressure acts on the dust, individual grains will be highly charged (Z ˜ 103+), and the grains are therefore strongly coupled to the gas through the ambient magnetic fields. Numerical hydrodynamic calculations were done to follow the evolution of mass-loss material. As the orbiting debris is driven outward by radiation pressure, the trail forms a spiral with initially high pitch angle (˜85°). The trails are compressed into thin ribbons in the radial direction initially by the radiation pressure gradients due to absorption within the trail. After reaching > 104 km s-1 radial velocity, the compression can be maintained by ram pressure due to an ambient gas of modest density (˜102 cm-3). Each of the stellar contrails will have mean column density ˜1019-1021 cm-2, volume density ˜108-109 cm-3, and thickness 1011-1012 cm along the line of sight to the AGN corresponding to parameters deduced from observations of the BAL clouds. Assuming minimal expansion perpendicular to the line of sight at the speed of sound, the width of the trails is 1015-1016 cm, or 102-103 times the line-of-sight depth. Since the UV-emitting accretion disk probably has a radius of about 2 × 1016 cm, a single trail will only partially cover the continuum, but for the column densities quoted above the observed absorption lines (e.g., C IV) will be optically thick with τ > 10. Since the contrails are nearly radial just after leaving the star when the maximum outward acceleration occurs, a large range of velocities (˜4000 km s-1) will be seen in absorption of the QSO light from each trail, and only a few disk-crossing trails are needed to account for the full width of broad absorption line troughs.

Exploiting OAuth 2.0: from User Delegation for OGC Services to a Generic Federation-as-a-Service Solution for Federated Identity Management

NASA Astrophysics Data System (ADS)

Kershaw, Philip; Jensen, Jens; Stephens, Ag; van Engen, Willem

2013-04-01

We explore an application of OAuth to enable user delegation for OGC-based services and the evolution of this solution to form part of a wider Federation-as-a-Service offering for federated identity management. OAuth has established itself in the commercial sector as a means for users to delegate access to secured resources under their control to third parties. It has also found its way into the academic and research domains as a solution for user delegation. Notable examples including the CILogon project for Teragrid in the US, and also, closer to the Earth Sciences, as part of the OGC Web Services, Phase 6 Testbed. Both are examples of OAuth 1.0 implementations. Version 2.0 has seen significant changes to this original specification which have not been without controversy but it has arguably provided a greater degree of flexibility in how it can be applied and the use cases that it can address. At CEDA (Centre for Environmental Data Archival, STFC), a Python implementation of OAuth 2.0 was made to explore these capabilities with a focus on providing a solution for user delegation for data access, processing and visualisation services for the Earth Observation and Climate sciences domains. The initial goal was to provide a means of delegating short-lived user credentials to trusted services along the same lines as the established approach of Proxy certificates widely used in Grid computing. For the OGC and other HTTP-based services employed by CEDA, OAuth makes a natural fit for this role, integrating with minimal impact on existing interfaces. Working implementations have been made for CEDA's COWS Web Processing Service and Web Map Service. Packaging the software and making it available in Open Source repositories together with the generic nature of the solution have made it readily exploitable in other application domains. At the Max Planck Institute for Psycholinguistics (Nijmegen, The Netherlands), the software will be used to integrate some tools in the CLARIN infrastructure*. Enhancements have been fedback to the package through this activity. Collaboration with STFC's Scientific Computing department has also seen this solution expand and evolve to support a more demanding set of use cases required to meet the needs for Contrail, an EU Framework 7 project. The goal of Contrail is to develop an Open Source solution for federating resources from multiple Cloud providers. Bringing the solution developed with OAuth together with technologies such as SAML and OpenID it has been possible to develop a generic suite of services to support federated access and identity management, a Federation-as-a-Service package. This is showing promise with trials with the EUDAT project. A deployment of the Contrail software is also planned for CEMS (the facility for Climate and Environmental Monitoring from Space), a new joint academic-industry led facility based at the STFC Harwell site providing access to large-volume Earth Observation and Climate datasets through a Cloud-based service model. * This work is part of the programme of BiG Grid, the Dutch e-Science Grid, which is financially supported by the Netherlands Organisation for Scientific Research, NWO.

Formation of Deep Electron Trap by Yb3+ Codoping Leads into Super-Long Persistent Luminescence in Ce3+-doped Yttrium Aluminum Gallium Garnet Phosphors.

PubMed

Ueda, Jumpei; Miyano, Shun; Tanabe, Setsuhisa

2018-05-23

The Y 3 Al 2 Ga 3 O 12 :Ce 3+ -Cr 3+ compound is one of the brightest persistent phosphors, but its persistent luminescence (PersL) duration is not so long due to the relatively shallow Cr 3+ electron trap. Comparing the vacuum referred binding energy of the electron trapping state by Cr 3+ and those by lanthanide ions, we selected Yb 3+ as a deeper electron trapping center. The Y 3 Al 2 Ga 3 O 12 :Ce 3+ -Yb 3+ phosphors show Ce 3+ :5d→4f green persistent luminescence after ceasing blue light excitation. The formation of Yb 2+ was confirmed by the increased intensity of absorption at 585 nm during the charging process. This result indicates that the Yb 3+ ions act as electron traps by capturing an electron. From the thermoluminescence glow curves, it was found the Yb 3+ trap makes much deeper electron trap with 1.01 eV depth than the Cr 3+ electron trap with 0.81 eV depth. This deeper Yb 3+ trap provides much slower detrapping rate of filled electron traps than the Cr 3+ -codoped persistent phosphor. In addition, by preparing transparent ceramics and optimizing Ce 3+ and Yb 3+ concentrations, the Y 3 Al 2 Ga 3 O 12 :Ce 3+ (0.2%)-Yb 3+ (0.1%) as-made transparent ceramic phosphor showed super long persistent luminescence for over 138.8 hours after ceasing blue light charging.

EndoAnchors to Resolve Persistent Type Ia Endoleak Secondary to Proximal Cuff With Parallel Graft Placement.

PubMed

Donselaar, Esmé J; van der Vijver-Coppen, Rozemarijn J; van den Ham, Leo H; Lardenoye, Jan Willem H P; Reijnen, Michel M P J

2016-02-01

To describe 2 patients with a distally migrated endograft causing a type Ia endoleak and treatment with a proximal cuff and chimney grafts that required EndoAnchors to finally seal the leak. Two men, ages 86 and 72 years, presented with stent-graft migration and type Ia endoleak at 5 and 15 years after endovascular repair, respectively. Both were treated with a proximal cuff in combination with a chimney graft to the left renal artery. In both cases, the type Ia endoleak persisted, likely due to gutter formation. Both patients were treated in the same setting with EndoAnchors that instantly resolved the endoleak. At 1-year follow-up, there was no recurrent endoleak or migration, with patent chimney grafts and renal arteries and stable renal function. EndoAnchors may effectively resolve a persistent type Ia endoleak arising from gutter formation after placement of a proximal cuff and chimney grafts. © The Author(s) 2015.

Muscle organizers in Drosophila: the role of persistent larval fibers in adult flight muscle development

NASA Technical Reports Server (NTRS)

Farrell, E. R.; Fernandes, J.; Keshishian, H.

1996-01-01

In many organisms muscle formation depends on specialized cells that prefigure the pattern of the musculature and serve as templates for myoblast organization and fusion. These include muscle pioneers in insects and muscle organizing cells in leech. In Drosophila, muscle founder cells have been proposed to play a similar role in organizing larval muscle development during embryogenesis. During metamorphosis in Drosophila, following histolysis of most of the larval musculature, there is a second round of myogenesis that gives rise to the adult muscles. It is not known whether muscle founder cells organize the development of these muscles. However, in the thorax specific larval muscle fibers do not histolyze at the onset of metamorphosis, but instead serve as templates for the formation of a subset of adult muscles, the dorsal longitudinal flight muscles (DLMs). Because these persistent larval muscle fibers appear to be functioning in many respects like muscle founder cells, we investigated whether they were necessary for DLM development by using a microbeam laser to ablate them singly and in combination. We found that, in the absence of the larval muscle fibers, DLMs nonetheless develop. Our results show that the persistent larval muscle fibers are not required to initiate myoblast fusion, to determine DLM identity, to locate the DLMs in the thorax, or to specify the total DLM fiber volume. However, they are required to regulate the number of DLM fibers generated. Thus, while the persistent larval muscle fibers are not obligatory for DLM fiber formation and differentiation, they are necessary to ensure the development of the correct number of fibers.

Kinetics of interior loop formation in semiflexible chains.

PubMed

Hyeon, Changbong; Thirumalai, D

2006-03-14

Loop formation between monomers in the interior of semiflexible chains describes elementary events in biomolecular folding and DNA bending. We calculate analytically the interior distance distribution function for semiflexible chains using a mean field approach. Using the potential of mean force derived from the distance distribution function we present a simple expression for the kinetics of interior looping by adopting Kramers theory. For the parameters, that are appropriate for DNA, the theoretical predictions in comparison with the case are in excellent agreement with explicit Brownian dynamics simulations of wormlike chain (WLC) model. The interior looping times (tauIC) can be greatly altered in the cases when the stiffness of the loop differs from that of the dangling ends. If the dangling end is stiffer than the loop then tauIC increases for the case of the WLC with uniform persistence length. In contrast, attachment of flexible dangling ends enhances rate of interior loop formation. The theory also shows that if the monomers are charged and interact via screened Coulomb potential then both the cyclization (tauc) and interior looping (tauIC) times greatly increase at low ionic concentration. Because both tauc and tauIC are determined essentially by the effective persistence length [lp(R)] we computed lp(R) by varying the range of the repulsive interaction between the monomers. For short range interactions lp(R) nearly coincides with the bare persistence length which is determined largely by the backbone chain connectivity. This finding rationalizes the efficacy of describing a number of experimental observations (response of biopolymers to force and cyclization kinetics) in biomolecules using WLC model with an effective persistence length.

Integration and Validation in Hybrid-Online Teacher Preparation: A Case Study of Persistence in a Native American Special Education Licensure Program

ERIC Educational Resources Information Center

Patterson, Donna Rose

2013-01-01

This study explored persistence through the experience of professional studies students in a special education licensure program. The context of the study was a graduate level teacher preparation program delivered in a hybrid format of face-to-face and online learning environments. The goal of the program was to prepare teachers from a Native…

The Persistence of Erroneous Familiarity in an Epileptic Male: Challenging Perceptual Theories of Deja Vu Activation

ERIC Educational Resources Information Center

O'Connor, Akira R.; Moulin, Christopher J. A.

2008-01-01

We report the case of a 39-year-old, temporal lobe epileptic male, MH. Prior to complex partial seizure, experienced up to three times a day, MH often experiences an aura experienced as a persistent sensation of deja vu. Data-driven theories of deja vu formation suggest that partial familiarity for the perceived stimulus is responsible for the…

Coinfection with Haemophilus influenzae promotes pneumococcal biofilm formation during experimental otitis media and impedes the progression of pneumococcal disease.

PubMed

Weimer, Kristin E D; Armbruster, Chelsie E; Juneau, Richard A; Hong, Wenzhou; Pang, Bing; Swords, W Edward

2010-10-01

Otitis media is an extremely common pediatric infection and is mostly caused by bacteria that are carried within the nasopharyngeal microbiota. It is clear that most otitis media cases involve simultaneous infection with multiple agents. Chinchillas were infected with nontypeable Haemophilus influenzae, Streptococcus pneumoniae, or a combination of both organisms, and the course of disease was compared. In vitro experiments were also performed to address how coinfection impacts biofilm formation. The incidence of systemic disease was reduced in coinfected animals, compared with those infected with pneumococcus alone. Pneumococci were present within surface-attached biofilms in coinfected animals, and a greater proportion of translucent colony type was observed in the coinfected animals. Because this colony type has been associated with pneumococcal biofilms, the impact of coinfection on pneumococcal biofilm formation was investigated. The results clearly show enhanced biofilm formation in vitro by pneumococci in the presence of H. influenzae. Based on these data, we conclude that coinfection with H. influenzae facilitates pneumococcal biofilm formation and persistence on the middle ear mucosal surface. This enhanced biofilm persistence correlates with delayed emergence of opaque colony variants within the bacterial population and a resulting decrease in systemic infection.

Rapid formation and evolution of an extreme haze episode in Northern China during winter 2015

PubMed Central

Sun, Yele; Chen, Chen; Zhang, Yingjie; Xu, Weiqi; Zhou, Libo; Cheng, Xueling; Zheng, Haitao; Ji, Dongsheng; Li, Jie; Tang, Xiao; Fu, Pingqing; Wang, Zifa

2016-01-01

We investigate the rapid formation and evolutionary mechanisms of an extremely severe and persistent haze episode that occurred in northern China during winter 2015 using comprehensive ground and vertical measurements, along with receptor and dispersion model analysis. Our results indicate that the life cycle of a severe winter haze episode typically consists of four stages: (1) rapid formation initiated by sudden changes in meteorological parameters and synchronous increases in most aerosol species, (2) persistent evolution with relatively constant variations in secondary inorganic aerosols and secondary organic aerosols, (3) further evolution associated with fog processing and significantly enhanced sulfate levels, and (4) clearing due to dry, cold north-northwesterly winds. Aerosol composition showed substantial changes during the formation and evolution of the haze episode but was generally dominated by regional secondary aerosols (53–67%). Our results demonstrate the important role of regional transport, largely from the southwest but also from the east, and of coal combustion emissions for winter haze formation in Beijing. Also, we observed an important downward mixing pathway during the severe haze in 2015 that can lead to rapid increases in certain aerosol species. PMID:27243909

Formation of Polychlorinated Biphenyls on Secondary Copper Production Fly Ash: Mechanistic Aspects and Correlation to Other Persistent Organic Pollutants

NASA Astrophysics Data System (ADS)

Jiang, Xiaoxu; Liu, Guorui; Wang, Mei; Zheng, Minghui

2015-09-01

Emission of unintentionally formed polychlorinated biphenyls (PCBs) from industrial thermal processes is a global issue. Because the production and use of technical PCB mixtures has been banned, industrial thermal processes have become increasingly important sources of PCBs. Among these processes, secondary copper smelting is an important PCB source in China. In the present study, the potential for fly ash-mediated formation of PCBs in the secondary copper industry, and the mechanisms involved, were studied in laboratory thermochemical experiments. The total PCB concentrations were 37-70 times higher than the initial concentrations. Thermochemical reactions on the fly ash amplified the potential toxic equivalents of PCBs. The formation of PCBs over time and the effect of temperature were investigated. Based on analyses of PCB homologue profiles with different reaction conditions, a chlorination mechanism was proposed for forming PCBs in addition to a de novo synthesis mechanism. The chlorination pathway was supported by close correlations between each pair of adjacent homologue groups. Formation of PCBs and multiple persistent organic pollutants, including polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated naphthalenes, occurred during the tests, indicating that these compounds may share similar formation mechanisms.

Soot and Sulfuric Acid from Aircraft: Is There Enough to Cause Detrimental Environmental E-kCTSs?

NASA Technical Reports Server (NTRS)

Pueschel, R. F.; Strawa, A. W.; Ferry, G. V.; Howard, S. D.; Verma, S.

1998-01-01

Aerosol from aircraft can affect the environment in three ways: First, soot aerosol has been implicated to cause Icing-tern ozone depletion at mid-latitudes in the lower stratosphere at a rate of approx. 5% per decade. This effect is in addition and unrelated to the polar ozone holes which are strongly influenced by heterogeneous chemistry on polar stratospheric clouds. Second, the most obvious effect of jet aircraft is the formation of visible contrails in the upper troposphere. The Salt Lake City region experienced an 8% increase in cirrus cloud cover over a 15-year period which covariates with an increase in regional commercial air traffic. If soot particles act as freezing nuclei to cause contrail formation heterogeneously, they would be linked to a secondary effect to cloud modification that very likely is climatologically important. Third, a buildup of soot aerosol could reduce the single scatter albedo of stratospheric aerosol from 0.993+0.004 to 0.98, a critical value that has been postulated to separate stratospheric cooling from warming. Thus arises an important question: Do aircraft emit sufficient amounts of soot to have detrimental effects and warrant emission controls? During the 1996 SUCCESS field campaign, we sampled aerosols in the exhaust wake of a Boeing 757 aircraft and determined emission indices for sulfuric acid (EI(sub H2SO4) = 9.0E-2 and 5.0E-1 g/kg (sub FUEL) for 75 and 675 ppm fuel-sulfur, respectively) and soot aerosol (2.2E-3 less than EI(sub SOOT) = l.lE-2 g/kg (sub FUEL)). The soot particle analysis accounted for their fractal nature, determined electron-microscopically, which enhanced the surface area by a factor of 26 and the volume 11-fold over equivalent-volume spheres. The corresponding fuel-sulfur to H2SO4 conversion efficiency was 10% (for 675 ppmm fuel-S) and 37% (for 75 ppmm fuel-S). Applying the H2SO4 emission index to the 1990 fuel use by the worlds commercial fleets of 1.3E11 kg, a conversion efficiency of 30% of 500 ppmm fuel-S would have led to an annual contribution to the atmospheric sulfur budget by aircraft of 2.E7 kg H2SO4. This is about one part in 1.E4 of anthropogenic sulfate from other sources. The soot emission index given above yielded a 1990 injection of soot aerosol by aircraft of 1.E6 kg. Thus, soot amounts to only five percent of the aerosol generated by aircraft. Its reactivity with ozone would have to be 20 times that of sulfuric acid particles to make it chemically significant. Nevertheless, the findings, of stratospheric soot loadings commensurate with aircraft fuel consumption, based on the emission index given above and the assumption of stratospheric residence times of the order of one year implicate aircraft as stratospheric polluters. A trend similar to soot of H2SO4 aerosol loading could not be deciphered, neither from in situ measurements nor SAGE II satellite extinction, against the "noise" due to volcanic eruptions. Observation of soot particles at 20 km altitude which, if emitted by aircraft were generated at 10-12 km altitude, suggests a displacement of those particles against gravity. Because eddy mixing is virtually absent in the lower stratosphere and isentropic mixing explains lofting to only about 15 km, radiometric forces acting on morphologically and chemically asymmetric soot particles must be considered a possibility. The consequence could be an extended residence time of soot against that of sulfuric acid aerosol that would lower the single scatter albedo with time.

Origin of the U(1) field mass in superconductors

NASA Astrophysics Data System (ADS)

Koizumi, Hiroyasu

2017-05-01

Recently, a new theory for superconductivity has been put forward, in which the persistent current generation is attributed to the emergent singularities of the electronic wave function that are created by the spin-twisting itinerant circular motion of electrons. The persistent current generated by this mechanism behaves in every respect like supercurrent in superconductors, yielding the flux quantum h/2e and the Josephson frequency 2eV/h, where h is Planck’s constant, -e is the electron charge, and V is the voltage across the Josephson junction. The mass generation of the U(1) gauge field (or the Meissner effect) in the new theory is due to the emergence of topological objects, ‘instantons’ generated by the single-valued requirement of the wave function in the presence of the emergent singularities. The current standard theory of superconductivity is based on the BCS theory, and explains the emergence of superconductivity as due to the global U(1) gauge symmetry breaking realized by the Cooper pair formation. The U(1) field mass generation is believed to be due to this global U(1) gauge symmetry breaking. However, the feasibility of this mechanism has been questioned since no known interaction can prepare the global U(1) symmetry broken state from the normal state. We argue here that the U(1) mass generation in the BCS superconductor can be attributed to the one by the instanton mentioned above if the Rashba spin-orbit interaction is added. Then, the occurrence of persistent current generation becomes due to the instanton formation, and the role of the Cooper pair formation is to stabilize the instanton by providing an energy gap for perturbative excitations. Upon forming the Cooper pair, the instanton is stabilized and persistent current generation becomes possible. Thus, the superconducting transition temperature coincides with the Cooper pair formation temperature.

In Vitro Emergence of High Persistence upon Periodic Aminoglycoside Challenge in the ESKAPE Pathogens

PubMed Central

Verstraeten, Natalie; Fauvart, Maarten

2016-01-01

Health care-associated infections present a major threat to modern medical care. Six worrisome nosocomial pathogens—Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.—are collectively referred to as the “ESKAPE bugs.” They are notorious for extensive multidrug resistance, yet persistence, or the phenotypic tolerance displayed by a variant subpopulation, remains underappreciated in these pathogens. Importantly, persistence can prevent eradication of antibiotic-sensitive bacterial populations and is thought to act as a catalyst for the development of genetic resistance. Concentration- and time-dependent aminoglycoside killing experiments were used to investigate persistence in the ESKAPE pathogens. Additionally, a recently developed method for the experimental evolution of persistence was employed to investigate adaptation to high-dose, extended-interval aminoglycoside therapy in vitro. We show that ESKAPE pathogens exhibit biphasic killing kinetics, indicative of persister formation. In vitro cycling between aminoglycoside killing and persister cell regrowth, evocative of clinical high-dose extended-interval therapy, caused a 37- to 213-fold increase in persistence without the emergence of resistance. Increased persistence also manifested in biofilms and provided cross-tolerance to different clinically important antibiotics. Together, our results highlight a possible drawback of intermittent, high-dose antibiotic therapy and suggest that clinical diagnostics might benefit from taking into account persistence. PMID:27185802

Characterization of multi-drug tolerant persister cells in Streptococcus suis

PubMed Central

2014-01-01

Background Persister cells constitute a subpopulation of dormant cells within a microbial population which are genetically identical but phenotypically different to regular cells. Notably, persister cells show an elevated tolerance to antimicrobial agents. Thus, they are considered to represent a microbial ‘bet-hedging’ strategy and are of particular importance in pathogenic bacteria. Results We studied the ability of the zoonotic pathogen Streptococcus (S.) suis to form multi-drug tolerant variants and identified persister cells dependent on the initial bacterial growth phase. We observed lower numbers of persisters in exponential phase cultures than in stationary growth phase populations. S. suis persister cells showed a high tolerance to a variety of antibiotics, and the phenotype was not inherited as tested with four passages of S. suis populations. Furthermore, we provide evidence that the persister phenotype is related to expression of genes involved in general metabolic pathways since we found higher numbers of persister cells in a mutant strain defective in the catabolic arginine deiminase system as compared to its parental wild type strain. Finally, we observed persister cell formation also in other S. suis strains and pathogenic streptococcal species. Conclusions Taken together, this is the first study that reports multi-drug tolerant persister cells in the zoonotic pathogen S. suis. PMID:24885389

Decadal trends in regional CO2 fluxes estimated from atmospheric inversions

NASA Astrophysics Data System (ADS)

Saeki, T.; Patra, P. K.

2016-12-01

Top-down approach (or atmospheric inversion) using atmospheric transport models and CO2 observations are an effective way to optimize surface fluxes at subcontinental scales and monthly time intervals. We used the CCSR/NIES/FRCGC AGCM-based Chemistry Transport Model (JAMSTEC's ACTM) and atmospheric CO2 concentrations at NOAA, CSIRO, JMA, NIES, NIES-MRI sites from Obspack GLOBALVIEW-CO2 data product (2013) for estimating CO2 fluxes for the period of 1990-2011. Carbon fluxes were estimated for 84 partitions (54 lands + 30 oceans) of the globe by using a Bayesian synthesis inversion framework. A priori fluxes are (1) atmosphere-ocean exchange from Takahashi et al. (2009), (2) 3-hourly terrestrial biosphere fluxes (annually balanced) from CASA model, and (3) fossil fuel fluxes from CDIAC global totals and EDGAR4.2 spatial distributions. Four inversion cases have been tested with 1) 21 sites (sites which have real data fraction of 90 % or more for 1989-2012), 2) 21 sites + CONTRAIL data, 3) 66 sites (over 70 % coverage), and 4) 157 sites. As a result of time-dependent inversions, mean total flux (excluding fossil fuel) for the period 1990-2011 is estimated to be -3.09 ±0.16 PgC/yr (mean and standard deviation of the four cases), where land (incl. biomass burning and land use change) and ocean absorb an average rate of -1.80 ±0.18 and -1.29 ±0.08 PgC/yr, respectively. The average global total sink from 1991-2000 to 2001-2010 increases by about 0.5 PgC/yr, mainly due to the increase in northern and tropical land sinks (Africa, Boreal Eurasia, East Asia and Europe), while ocean sinks show no clear trend. Inversion with CONTRAIL data estimates large positive flux anomalies in late 1997 associated with the 1997/98 El-Nino, while inversion without CONTARIL data between Japan and Australia fails to estimate such large anomalies. Acknowledgements. This work is supported by the Environment Research and Technology Development Fund (2-1401) of the Ministry of the Environment, Japan. We thank all measurement groups for submitting CO2 concentration data to the obspack-GLOBALVIEW product.

Family Matters: Familial Support and Science Identity Formation for African American Female STEM Majors

ERIC Educational Resources Information Center

Parker, Ashley Dawn

2013-01-01

This research seeks to understand the experiences of African American female undergraduates in STEM. It investigates how familial factors and science identity formation characteristics influence persistence in STEM while considering the duality of African American women's status in society. This phenomenological study was designed using critical…

Enhancing Formative Assessment Practice and Encouraging Middle School Mathematics Engagement and Persistence

ERIC Educational Resources Information Center

Beesley, Andrea D.; Clark, Tedra F.; Dempsey, Kathleen; Tweed, Anne

2018-01-01

In the transition to middle school, and during the middle school years, students' motivation for mathematics tends to decline from what it was during elementary school. Formative assessment strategies in mathematics can help support motivation by building confidence for challenging tasks. In this study, the authors developed and piloted a…

Effect of Particulate Matter Mineral Composition on Environmentally Persistent Free Radical (EPFR) Formation.

PubMed

Feld-Cook, Elisabeth E; Bovenkamp-Langlois, Lisa; Lomnicki, Slawo M

2017-09-19

Environmentally Persistent Free Radicals (EPFRs) are newly discovered, long-lived surface bound radicals that form on particulate matter and combustion borne particulates, such as fly ash. Human exposure to such particulates lead to translocation into the lungs and heart resulting in cardio-vascular and respiratory disease through the production of reactive oxygen species. Analysis of some waste incinerator fly ashes revealed a significant difference between their EPFR contents. Although EPFR formation occurs on the metal domains, these differences were correlated with the altering concentration of calcium and sulfur. To analyze these phenomena, surrogate fly ashes were synthesized to mimic the presence of their major mineral components, including metal oxides, calcium, and sulfur. The results of this study led to the conclusion that the presence of sulfates limits formation of EPFRs due to inhibition or poisoning of the transition metal active sites necessary for their formation. These findings provide a pathway toward understanding differences in EPFR presence on particulate matter and uncover the possibility of remediating EPFRs from incineration and hazardous waste sites.

The persistence of erroneous familiarity in an epileptic male: challenging perceptual theories of déjà vu activation.

PubMed

O'Connor, Akira R; Moulin, Christopher J A

2008-11-01

We report the case of a 39-year-old, temporal lobe epileptic male, MH. Prior to complex partial seizure, experienced up to three times a day, MH often experiences an aura experienced as a persistent sensation of déjà vu. Data-driven theories of déjà vu formation suggest that partial familiarity for the perceived stimulus is responsible for the sensation. Consequently, diverting attention away from this stimulus should cause the sensation to dissipate. MH, whose sensations of déjà vu persist long enough for him to shift his perceptual focus a number of times during the experience, spontaneously reports that these shifts make no difference to the sensation experienced. This novel observation challenges data-driven theories of déjà vu formation which have been used to explain the occurrence of déjà vu in those with temporal lobe epilepsy and the general population. Clearly, in epilepsy, erratic neuronal firing is the likely contributor, and in this paper we postulate that such brain firing causes higher-order erroneous 'cognitive feelings'. We tentatively extend this account to the general population. Rather than being a reaction to familiar elements in perceptual stimuli, déjà vu is likely to be the result of a cognitive feeling borne of the erroneous activation of neural familiarity circuits such as the parahippocampal gyrus, persisting as long as this activation persists.

Behavioral self-organization underlies the resilience of a coastal ecosystem.

PubMed

de Paoli, Hélène; van der Heide, Tjisse; van den Berg, Aniek; Silliman, Brian R; Herman, Peter M J; van de Koppel, Johan

2017-07-25

Self-organized spatial patterns occur in many terrestrial, aquatic, and marine ecosystems. Theoretical models and observational studies suggest self-organization, the formation of patterns due to ecological interactions, is critical for enhanced ecosystem resilience. However, experimental tests of this cross-ecosystem theory are lacking. In this study, we experimentally test the hypothesis that self-organized pattern formation improves the persistence of mussel beds ( Mytilus edulis ) on intertidal flats. In natural beds, mussels generate self-organized patterns at two different spatial scales: regularly spaced clusters of mussels at centimeter scale driven by behavioral aggregation and large-scale, regularly spaced bands at meter scale driven by ecological feedback mechanisms. To test for the relative importance of these two spatial scales of self-organization on mussel bed persistence, we conducted field manipulations in which we factorially constructed small-scale and/or large-scale patterns. Our results revealed that both forms of self-organization enhanced the persistence of the constructed mussel beds in comparison to nonorganized beds. Small-scale, behaviorally driven cluster patterns were found to be crucial for persistence, and thus resistance to wave disturbance, whereas large-scale, self-organized patterns facilitated reformation of small-scale patterns if mussels were dislodged. This study provides experimental evidence that self-organization can be paramount to enhancing ecosystem persistence. We conclude that ecosystems with self-organized spatial patterns are likely to benefit greatly from conservation and restoration actions that use the emergent effects of self-organization to increase ecosystem resistance to disturbance.

Behavioral self-organization underlies the resilience of a coastal ecosystem

PubMed Central

de Paoli, Hélène; van der Heide, Tjisse; van den Berg, Aniek; Silliman, Brian R.; Herman, Peter M. J.

2017-01-01

Self-organized spatial patterns occur in many terrestrial, aquatic, and marine ecosystems. Theoretical models and observational studies suggest self-organization, the formation of patterns due to ecological interactions, is critical for enhanced ecosystem resilience. However, experimental tests of this cross-ecosystem theory are lacking. In this study, we experimentally test the hypothesis that self-organized pattern formation improves the persistence of mussel beds (Mytilus edulis) on intertidal flats. In natural beds, mussels generate self-organized patterns at two different spatial scales: regularly spaced clusters of mussels at centimeter scale driven by behavioral aggregation and large-scale, regularly spaced bands at meter scale driven by ecological feedback mechanisms. To test for the relative importance of these two spatial scales of self-organization on mussel bed persistence, we conducted field manipulations in which we factorially constructed small-scale and/or large-scale patterns. Our results revealed that both forms of self-organization enhanced the persistence of the constructed mussel beds in comparison to nonorganized beds. Small-scale, behaviorally driven cluster patterns were found to be crucial for persistence, and thus resistance to wave disturbance, whereas large-scale, self-organized patterns facilitated reformation of small-scale patterns if mussels were dislodged. This study provides experimental evidence that self-organization can be paramount to enhancing ecosystem persistence. We conclude that ecosystems with self-organized spatial patterns are likely to benefit greatly from conservation and restoration actions that use the emergent effects of self-organization to increase ecosystem resistance to disturbance. PMID:28696313

Surviving colorectal cancer: long-term, persistent ostomy-specific concerns and adaptations.

PubMed

Sun, Virginia; Grant, Marcia; McMullen, Carmit K; Altschuler, Andrea; Mohler, M Jane; Hornbrook, Mark C; Herrinton, Lisa J; Baldwin, Carol M; Krouse, Robert S

2013-01-01

The purpose of this article was to describe persistent ostomy-specific concerns and adaptations in long-term (>5 years) colorectal cancer survivors with ostomies. Thirty-three colorectal cancer survivors who participated in 8 gender- and health-related quality of life stratified focus groups and 130 colorectal cancer survivors who provided written comments to 2 open-ended questions on ostomy location and pouch problems participated in the study. Data were collected on health maintenance organization members in Oregon, southwestern Washington, and northern California. Qualitative data were analyzed for the 8 focus groups and written comments from 2 open-ended survey questions. Discussions from the focu s groups were recorded, transcribed, and analyzed using content analysis. Written content from the open-ended questions was derived from a mailed questionnaire on health-related quality of life in survivors with ostomies and analyzed using content analysis. Discussions related to persistent ostomy-related issues more than 5 years after formation were common. Persistent ostomy-related issues were focused on clothing restrictions and adaptations, dietary concerns, issues related to ostomy equipment and self-care, and the constant need to find solutions to adjust and readjust to living with an ostomy. Ostomy-specific concerns persist 5 years and more for long-term colorectal cancer survivors after initial ostomy formation. Adaptations tend to be individualized and based on trial and error. Findings underscore the need to develop long-term support mechanisms that survivors can access to promote better coping and adjustment to living with an ostomy.

Environmental reservoirs and mechanisms of persistence of Vibrio cholerae

PubMed Central

Lutz, Carla; Erken, Martina; Noorian, Parisa; Sun, Shuyang; McDougald, Diane

2013-01-01

It is now well accepted that Vibrio cholerae, the causative agent of the water-borne disease cholera, is acquired from environmental sources where it persists between outbreaks of the disease. Recent advances in molecular technology have demonstrated that this bacterium can be detected in areas where it has not previously been isolated, indicating a much broader, global distribution of this bacterium outside of endemic regions. The environmental persistence of V. cholerae in the aquatic environment can be attributed to multiple intra- and interspecific strategies such as responsive gene regulation and biofilm formation on biotic and abiotic surfaces, as well as interactions with a multitude of other organisms. This review will discuss some of the mechanisms that enable the persistence of this bacterium in the environment. In particular, we will discuss how V. cholerae can survive stressors such as starvation, temperature, and salinity fluctuations as well as how the organism persists under constant predation by heterotrophic protists. PMID:24379807

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

PubMed Central

Madeira, Fernanda Fernandez; Borsatto, Kelly Cristine; Lima, Anna Claudia Campaner; Ravazi, Amanda; de Oliveira, Jader; da Rosa, João Aristeu; de Azeredo-Oliveira, Maria Tercília Vilela; Alevi, Kaio Cesar Chaboli

2016-01-01

All species of triatomines are considered potential vectors of Chagas disease and the reproductive biology of these bugs has been studied by different approaches. In 1999, nucleolar persistence during meiosis was observed in the subfamily for the first time. Recently, it has been observed that all species within the genus Rhodnius exhibit the same phenomenon, suggesting that it may be a synapomorphy of the triatomines. Thus, this article aims to analyze the nucleolar behavior during spermatogenesis of 59 triatomine species. All analyzed species exhibited nucleolar persistence during meiosis. Recently, it has been suggested that nucleolar persistence may be fundamental for the spermatogenesis of these vectors, since it is related to the formation of the chromatoid body. Therefore, we emphasize that this phenomenon is a peculiarity of the Triatominae subfamily and that further studies are required to analyze whether the nucleolar material that persists is active. PMID:27645782

Technical Evaluation Report

DTIC Science & Technology

2005-04-01

Job, asked if the time constant of tinnitus persistence after an acute acoustic exposure can be predicted from data on hearing thresholds and/or from...individuals whose tinnitus subsides before 72 hours, post-exposure, and individuals whose tinnitus persists Technical Evaluation Report T - 8 RTO-MP-HFM-123...induces formation of vasoactive lipid peroxidation products in the cochlea. Brain Research, 878, 163-173. [10] Rao, D. B, Moore, D. R., Reinke, L. A

Effect of copper oxide concentration on the formation and persistency of environmentally persistent free radicals (EPFRs) in particulates.

PubMed

Kiruri, Lucy W; Khachatryan, Lavrent; Dellinger, Barry; Lomnicki, Slawo

2014-02-18

Environmentally persistent free radicals (EPFRs) are formed by the chemisorption of substituted aromatics on metal oxide surfaces in both combustion sources and superfund sites. The current study reports the dependency of EPFR yields and their persistency on metal loading in particles (0.25, 0.5, 0.75, 1, 2, and 5% CuO/silica). The EPFRs were generated through exposure of particles to three adsorbate vapors at 230 °C: phenol, 2-monochlorophenol (2-MCP), and dichlorobenzene (DCBz). Adsorption resulted in the formation of surface-bound phenoxyl- and semiquinoine-type radicals with characteristic EPR spectra displaying a g value ranging from ∼ 2.0037 to 2.006. The highest EPFR yield was observed for CuO concentrations between 1 and 3% in relation to MCP and phenol adsorption. However, radical density, which is expressed as the number of radicals per copper atom, was highest at 0.75-1% CuO loading. For 1,2-dichlorobenzene adsorption, radical concentration increased linearly with decreasing copper content. At the same time, a qualitative change in the radicals formed was observed--from semiquinone to chlorophenoxyl radicals. The two longest lifetimes, 25 and 23 h, were observed for phenoxyl-type radicals on 0.5% CuO and chlorophenoxyl-type radicals on 0.75% CuO, respectively.

High potential for formation and persistence of chimeras following aggregated larval settlement in the broadcast spawning coral, Acropora millepora

PubMed Central

Puill-Stephan, E.; van Oppen, M. J. H.; Pichavant-Rafini, K.; Willis, B. L.

2012-01-01

In sessile modular marine invertebrates, chimeras can originate from fusions of closely settling larvae or of colonies that come into contact through growth or movement. While it has been shown that juveniles of brooding corals fuse under experimental conditions, chimera formation in broadcast spawning corals, the most abundant group of reef corals, has not been examined. This study explores the capacity of the broadcast spawning coral Acropora millepora to form chimeras under experimental conditions and to persist as chimeras in the field. Under experimental conditions, 1.5-fold more larvae settled in aggregations than solitarily, and analyses of nine microsatellite loci revealed that 50 per cent of juveniles tested harboured different genotypes within the same colony. Significantly, some chimeric colonies persisted for 23 months post-settlement, when the study ended. Genotypes within persisting chimeric colonies all showed a high level of relatedness, whereas rejecting colonies displayed variable levels of relatedness. The nearly threefold greater sizes of chimeras compared with solitary juveniles, from settlement through to at least three months, suggest that chimerism is likely to be an important strategy for maximizing survival of vulnerable early life-history stages of corals, although longer-term studies are required to more fully explore the potential benefits of chimerism. PMID:21752820

High potential for formation and persistence of chimeras following aggregated larval settlement in the broadcast spawning coral, Acropora millepora.

PubMed

Puill-Stephan, E; van Oppen, M J H; Pichavant-Rafini, K; Willis, B L

2012-02-22

In sessile modular marine invertebrates, chimeras can originate from fusions of closely settling larvae or of colonies that come into contact through growth or movement. While it has been shown that juveniles of brooding corals fuse under experimental conditions, chimera formation in broadcast spawning corals, the most abundant group of reef corals, has not been examined. This study explores the capacity of the broadcast spawning coral Acropora millepora to form chimeras under experimental conditions and to persist as chimeras in the field. Under experimental conditions, 1.5-fold more larvae settled in aggregations than solitarily, and analyses of nine microsatellite loci revealed that 50 per cent of juveniles tested harboured different genotypes within the same colony. Significantly, some chimeric colonies persisted for 23 months post-settlement, when the study ended. Genotypes within persisting chimeric colonies all showed a high level of relatedness, whereas rejecting colonies displayed variable levels of relatedness. The nearly threefold greater sizes of chimeras compared with solitary juveniles, from settlement through to at least three months, suggest that chimerism is likely to be an important strategy for maximizing survival of vulnerable early life-history stages of corals, although longer-term studies are required to more fully explore the potential benefits of chimerism.

Computationally derived rules for persistence of C60 nanowires on recumbent pentacene bilayers.

PubMed

Cantrell, Rebecca A; James, Christine; Clancy, Paulette

2011-08-16

The tendency for C(60) nanowires to persist on two monolayers of recumbent pentacene is studied using molecular dynamics (MD) simulations. A review of existing experimental literature for the tilt angle adopted by pentacene on noble metal surfaces shows that studies cover a limited range from 55° to 90°, motivating simulation studies of essentially the entire range of tilt angles (10°-90°) to predict the optimum surface tilt angle for C(60) nanowire formation. The persistence of a 1D nanowire depends sensitively on this tilt angle, the amount of initial tensile strain, and the presence of surface step edges. At room temperature, C(60) nanowires oriented along the pentacene short axes persist for several nanoseconds and are more likely to occur if they reside between, or within, pentacene rows for ϕ ≤ ∼60°. The likelihood of this persistence increases the smaller the tilt angle. Nanowires oriented along the long axes of pentacene molecules are unlikely to form. The limit of stability of nanowires was tested by raising the temperature to 400 K. Nanowires located between pentacene rows survived this temperature rise, but those located initially within pentacene rows are only stable in the range ϕ(1) = 30°-50°. Flatter pentacene surfaces, that is, tilt angles above about 60°, are subject to disorder caused by C(60) molecules "burrowing" into the pentacene surface. An initial strain of 5% applied to the C(60) nanowires significantly decreases the likelihood of nanowire persistence. In contrast, any appreciable surface roughness, even by half a monolayer in height of a third pentacene monolayer, strongly enhances the likelihood of nanowire formation due to the strong binding energy of C(60) molecules to step edges.

Specific Human and Candida Cellular Interactions Lead to Controlled or Persistent Infection Outcomes during Granuloma-Like Formation

PubMed Central

Misme-Aucouturier, Barbara; Albassier, Marjorie

2016-01-01

ABSTRACT A delayed type of multicellular process could be crucial during chronic candidiasis in determining the course of infection. This reaction, consisting of organized immune cells surrounding the pathogen, initiates an inflammatory response to avoid fungal dissemination. The goal of the present study was to examine, at an in vitro cellular scale, Candida and human immune cell interaction dynamics during a long-term period. By challenging human peripheral blood immune cells from 10 healthy donors with 32 Candida albicans and non-albicans (C. glabrata, C. tropicalis, C. parapsilosis, C. dubliniensis, C. lusitaniae, C. krusei, and C. kefyr) clinical isolates, we showed that Candida spp. induced the formation of granuloma-like structures within 6 days after challenge, but their sizes and the respective fungal burdens differed according to the Candida species. These two parameters are positively correlated. Phenotypic characteristics, such as hypha formation and higher axenic growth rate, seem to contribute to yeast persistence within granuloma-like structures. We showed an interindividual variability of the human response against Candida spp. Higher proportions of neutrophils and elevated CD4+/CD8+ T cell ratios during the first days after challenge were correlated with early production of gamma interferon (IFN-γ) and associated with controlled infection. In contrast, the persistence of Candida could result from upregulation of proinflammatory cytokines such as interleukin-6 (IL-6), IFN-γ, and tumor necrosis factor alpha (TNF-α) and a poor anti-inflammatory negative feedback (IL-10). Importantly, regulatory subsets of NK cells and CD4lo CD8hi doubly positive (DP) lymphocytes at late stage infiltrate granuloma-like structures and could correlate with the IL-10 and TNF-α production. These data offer a base frame to explain cellular events that guide infection control or fungal persistence. PMID:27799331

Coherex WAVECREST I Left Atrial Appendage Occlusion Study

ClinicalTrials.gov

2015-01-13

Non-valvular Paroxysmal, Persistent, or Permanent Atrial Fibrillation; LAA Anatomy Amenable to Treatment by Percutaneous Technique; Anticoagulation Indication for Potential Thrombus Formation in the Left Atrium

In Vitro Emergence of High Persistence upon Periodic Aminoglycoside Challenge in the ESKAPE Pathogens.

PubMed

Michiels, Joran Elie; Van den Bergh, Bram; Verstraeten, Natalie; Fauvart, Maarten; Michiels, Jan

2016-08-01

Health care-associated infections present a major threat to modern medical care. Six worrisome nosocomial pathogens-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.-are collectively referred to as the "ESKAPE bugs." They are notorious for extensive multidrug resistance, yet persistence, or the phenotypic tolerance displayed by a variant subpopulation, remains underappreciated in these pathogens. Importantly, persistence can prevent eradication of antibiotic-sensitive bacterial populations and is thought to act as a catalyst for the development of genetic resistance. Concentration- and time-dependent aminoglycoside killing experiments were used to investigate persistence in the ESKAPE pathogens. Additionally, a recently developed method for the experimental evolution of persistence was employed to investigate adaptation to high-dose, extended-interval aminoglycoside therapy in vitro We show that ESKAPE pathogens exhibit biphasic killing kinetics, indicative of persister formation. In vitro cycling between aminoglycoside killing and persister cell regrowth, evocative of clinical high-dose extended-interval therapy, caused a 37- to 213-fold increase in persistence without the emergence of resistance. Increased persistence also manifested in biofilms and provided cross-tolerance to different clinically important antibiotics. Together, our results highlight a possible drawback of intermittent, high-dose antibiotic therapy and suggest that clinical diagnostics might benefit from taking into account persistence. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

[Activating effect of cyclophosphane at late stages of persistence of the tick-borne encephalitis virus].

PubMed

Frolova, T V; Pogodina, V V; Larina, G I; Frolova, M P; Karmysheva, V Ia

1982-01-01

Conditions of activation of persistent infection caused by subcutaneous inoculation of Syrian hamsters with the B-383 and Vasilchenko strains of tick-borne encephalitis virus (TBE) were studied. After 2 administrations of cyclophosphane (CP) on day 170 of infection clinically manifest disease developed in some animals with increasingly severe pathomorphological lesions in the CNS. Several variants of activated TBE virus were isolated from brains and spleens of CP-treated hamsters. The activation of persistent infection was observed in the presence of marked decreased of humoral immunity level, weight of the thymus, and values of spontaneous rosette-formation.

Formation of environmentally persistent free radicals as the mechanism for reduced catechol degradation on hematite-silica surface under UV irradiation.

PubMed

Li, Hao; Pan, Bo; Liao, Shaohua; Zhang, Di; Xing, Baoshan

2014-05-01

Iron is rich in soils, and is recently reported to form stable complexes with organic free radicals, generating environmentally persistent free radicals (EPFRs). The observation may challenge the common viewpoint that iron is an effective catalyst to facilitate the degradation of various organic chemicals. But no study was specifically designed to investigate the possible inhibited degradation of organic chemicals because of the formation of EPFRs in dry environment. We observed that catechol degradation under UV irradiation was decreased over 20% in silica particles coated with 1% hematite in comparison to uncoated silica particles. Stabilized semiquinone or quinine and phenol radicals were involved in HMT-silica system. EPFR formation was thus the reason for the reduced catechol degradation on HMT-silica surface under UV irradiation at ambient temperature. EPFRs should be incorporated in the studies of organic contaminants geochemical behavior, and will be a new input in their environmental fate modeling. Copyright © 2014 Elsevier Ltd. All rights reserved.

The formation of nitrogen-containing organic oxidation products in a heavily polluted urban environment

NASA Astrophysics Data System (ADS)

Chen, Q.; Cheng, X.; Zheng, Y.; Li, Y.; Zhu, T.; Zhang, Q.; Canagaratna, M. R.; Nowark, J.; Worsnop, D. R.

2017-12-01

Nitrogen-containing organic oxidation products are important species that may contribute to secondary organic aerosol and to redistribute nitrogen oxides through photolysis and oxidation. We deployed a nitrate ion chemical ionization time-of-flight mass spectrometer and a long time-of-flight aerosol mass spectrometer in Beijing for various seasons to study the nitrogen-containing organic species in both gas and particle phases. High concentrations of nitrated phenols were observed in both winter and summer, due to contributions from both primary and secondary sources. The concentrations of gaseous dinitrophenols tracked the severe haze events and correlated well with many highly oxygenated organic molecules. We also identified mass spectral tracers for quantifying organic nitrates. Significant photochemical production of nitrate and organic nitrates were evident during severe haze events, which may contribute to persistent particle formation. The findings are consistent with observed high OH turnover rates, highlighting the importance of gaseous oxidation pathways on persistent particle formation during haze.

Effect of persistent trace compounds in landfill gas on engine performance during energy recovery: a case study.

PubMed

Sevimoğlu, Orhan; Tansel, Berrin

2013-01-01

Performances of gas engines operated with landfill gas (LFG) are affected by the impurities in the LFG, reducing the economic viability of energy recovery. The purpose of this study was to characterize the trace compounds in the LFG at the Odayeri Landfill, Istanbul, Turkey which is used for energy recovery. Composite gas samples were collected and analyzed for trace compounds (hydrocarbons, siloxanes, and volatile halogenated hydrocarbons) over a 3-year period. Trace compounds entering the gas engines, their impact on the engine performance were evaluated. The operational problems included deposit formation in the combustion chamber, turbocharger, and intercooler of engine before the scheduled maintenance times. High levels of hydrogen sulfide, as well as chlorinated and fluorinated compounds cause corrosion of the engine parts and decrease life of the engine oils. Persistence of siloxanes results in deposit formation, increasing engine maintenance costs. Pretreatment of LFG is necessary to protect the engines at the waste-to-energy facilities with persistence levels of siloxanes and volatile halogenated hydrocarbons. Copyright © 2012 Elsevier Ltd. All rights reserved.

Treatment of a Persistent False Lumen with Aneurysm Formation Following Surgical Repair of Type A Dissection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jeganathan, Reubendra, E-mail: reubenj@hotmail.com; Kennedy, Peter; MacGowan, Simon

2007-06-15

We describe the case of a 68-year-old man who developed aneurysmal dilatation of the proximal descending thoracic aorta 8 years after repair of a type A dissection. The aneurysm was due to an anastomotic leak at the distal end of the previous repair in the ascending aorta with antegrade perfusion of the false lumen. Surgical repair of the anastomotic leak partially obliterated the false lumen and computed tomography scan demonstrated thrombosis in a large proportion of the false lumen aneurysm. Follow-up with surveillance scans showed persistent filling of this aneurysm due to retrograde flow of blood within the false lumen.more » Coil embolization of the false lumen within the thoracic aorta was performed which successfully thrombosed the aneurysm with a reduction in diameter. Late aneurysm formation may complicate type A dissection repairs during follow-up due to a persistent false lumen, especially if there is an anastomotic leak. This case report describes a strategy to deal with this difficult clinical problem.« less

The Formation of a Small-Scale Filament After Flux Emergence on the Quiet Sun

NASA Astrophysics Data System (ADS)

Chen, Hechao; Yang, Jiayan; Yang, Bo; Ji, Kaifan; Bi, Yi

2018-06-01

We present observations of the formation process of a small-scale filament on the quiet Sun during 5 - 6 February 2016 and investigate its formation cause. Initially, a small dipole emerged, and its associated arch filament system was found to reconnect with overlying coronal fields accompanied by numerous extreme ultraviolet bright points. When the bright points faded, many elongated dark threads formed and bridged the positive magnetic element of the dipole and the external negative network fields. Interestingly, an anticlockwise photospheric rotational motion (PRM) set in within the positive endpoint region of the newborn dark threads following the flux emergence and lasted for more than 10 hours. Under the drive of the PRM, these dispersive dark threads gradually aligned along the north-south direction and finally coalesced into an inverse S-shaped filament. Consistent with the dextral chirality of the filament, magnetic helicity calculations show that an amount of negative helicity was persistently injected from the rotational positive magnetic element and accumulated during the formation of the filament. These observations suggest that twisted emerging fields may lead to the formation of the filament via reconnection with pre-existing fields and release of its inner magnetic twist. The persistent PRM might trace a covert twist relaxation from below the photosphere to the low corona.

Coinfection with Haemophilus influenzae promotes pneumococcal biofilm formation during experimental otitis media and impedes the progression of pneumococcal disease

PubMed Central

Weimer, Kristin E.D.; Armbruster, Chelsie E.; Juneau, Richard A.; Hong, Wenzhou; Pang, Bing; Swords, W. Edward

2010-01-01

Background Otitis media is an extremely common pediatric infection, and is mostly caused by bacteria that are carried within the nasopharyngeal microbiota. It is clear that most otitis media cases involve simultaneous infection with multiple agents. Methods Chinchillas were infected with nontypeable Haemophilus influenzae, Streptococcus pneumoniae, or a combination of both organisms, and the course of disease was compared. In vitro experiments were also performed to address how coinfection impacts biofilm formation. Results The incidence of systemic disease was reduced in coinfected animals as compared to those infected with pneumococcus alone. Pneumococci were present within surface-attached biofilms in coinfected animals, and a greater proportion of translucent colony type was observed in the coinfected animals. As this colony type has been associated with pneumococcal biofilms, the impact of coinfection on pneumococcal biofilm formation was investigated. The results clearly show enhanced biofilm formation in vitro by pneumococci in the presence of H. influenzae. Conclusions Based on these data, we conclude that coinfection with H. influenzae facilitates pneumococcal biofilm formation and persistence on the middle-ear mucosal surface. This enhanced biofilm persistence correlates with delayed emergence of opaque colony variants within the bacterial population, and a resulting decrease in systemic infection. PMID:20715928

Low-frequency oscillations of the East Asia-Pacific teleconnection pattern and their impacts on persistent heavy precipitation in the Yangtze-Huai River valley

NASA Astrophysics Data System (ADS)

Li, Lei; Zhai, Panmao; Chen, Yang; Ni, Yunqi

2016-06-01

Based on the daily reanalysis data from NCEP-NCAR and daily precipitation data from the China National Meteorological Information Center, an ensemble empirical mode decomposition method is employed to extract the predominant oscillation modes of the East Asia-Pacific (EAP) teleconnection pattern. The influences of these low-frequency modes on persistent heavy precipitation in the Yangtze-Huai River (YHR) valley are investigated. The results indicate that the EAP pattern and rainfall in YHR valley both exhibit remarkable 10-30- and 30-60-day oscillations. The impacts of the EAP pattern on the YHR persistent heavy precipitation can be found on both the 10-30- and 30-60-day timescales—the 10-30-day scale for most cases. Composite analysis indicates that, on the 10-30-day timescale, formation of the EAP pattern in the lower and middle troposphere is determined by convective systems near the tropical western Pacific; whereas in the middle troposphere, the phase transition is jointly contributed by both the dispersion of zonal wave energies at higher latitudes and convective systems over the South China Sea. In the context of the 10-30-day EAP pattern, the anomalously abundant moisture is transported by an anomalous subtropical anticyclone system, and strong moisture convergence results from that anomalous anticyclone system and a cyclonic system in the midlatitude East Asia. Such a combination of systems persists for at least three days, contributing to the formation of persistent heavy precipitation in the YHR valley.

Surviving Colorectal Cancer: Long-Term, Persistent Ostomy-Specific Concerns and Adaptations

PubMed Central

Sun, Virginia; Grant, Marcia; McMullen, Carmit K.; Altschuler, Andrea; Mohler, M. Jane; Hornbrook, Mark C.; Herrinton, Lisa J.; Baldwin, Carol M.; Krouse, Robert S.

2012-01-01

Purpose The purpose of this paper is to describe persistent ostomy-specific concerns and adaptations in long-term (> 5 years) colorectal cancer survivors with ostomies. Subjects and Settings Thirty three colorectal cancer survivors who participated in eight gender- and health related Quality of life (HRQOL) stratified focus groups and 130 colorectal cancer survivors who provided written comments to two open-ended questions on ostomy location and pouch problems participated in the study. Data were collected on health maintenance organization members in Oregon, southwestern Washington and northern California. Methods Qualitative data were analyzed for the 8 focus groups and written comments from 2 open-ended survey questions. Discussions from the focus groups were recorded, transcribed and analyzed using content analysis. Written content from the open-ended questions was derived from a mailed questionnaire on health related quality of life in survivors with ostomies and analyzed using content analysis. Results Discussions related to persistent ostomy-related issues more than 5 years after formation were common. Persistent ostomy-related issues were focused on clothing restrictions and adaptations, dietary concerns, issues related to ostomy equipment and self-care, and the constant need to find solutions to adjust and re-adjust to living with an ostomy. Conclusions Ostomy-specific concerns persist 5 years and more for long-term colorectal cancer survivors after initial ostomy formation. Adaptations tend to be individualized and based on trial and error. Findings underscore the need to develop long-term support mechanisms that survivors can access to promote better coping and adjustment to living with an ostomy. PMID:23222968

Persistent increased PKMζ in long-term and remote spatial memory.

PubMed

Hsieh, Changchi; Tsokas, Panayiotis; Serrano, Peter; Hernández, A Iván; Tian, Dezhi; Cottrell, James E; Shouval, Harel Z; Fenton, André Antonio; Sacktor, Todd Charlton

2017-02-01

PKMζ is an autonomously active PKC isoform that is thought to maintain both LTP and long-term memory. Whereas persistent increases in PKMζ protein sustain the kinase's action in LTP, the molecular mechanism for the persistent action of PKMζ during long-term memory has not been characterized. PKMζ inhibitors disrupt spatial memory when introduced into the dorsal hippocampus from 1day to 1month after training. Therefore, if the mechanisms of PKMζ's persistent action in LTP maintenance and long-term memory were similar, persistent increases in PKMζ would last for the duration of the memory, far longer than most other learning-induced gene products. Here we find that spatial conditioning by aversive active place avoidance or appetitive radial arm maze induces PKMζ increases in dorsal hippocampus that persist from 1day to 1month, coinciding with the strength and duration of memory retention. Suppressing the increase by intrahippocampal injections of PKMζ-antisense oligodeoxynucleotides prevents the formation of long-term memory. Thus, similar to LTP maintenance, the persistent increase in the amount of autonomously active PKMζ sustains the kinase's action during long-term and remote spatial memory maintenance. Copyright © 2016. Published by Elsevier Inc.

Persistent agents in Axelrod's social dynamics model

NASA Astrophysics Data System (ADS)

Reia, Sandro M.; Neves, Ubiraci P. C.

2016-01-01

Axelrod's model of social dynamics has been studied under the effect of external media. Here we study the formation of cultural domains in the model by introducing persistent agents. These are agents whose cultural traits are not allowed to change but may be spread through local neighborhood. In the absence of persistent agents, the system is known to present a transition from a monocultural to a multicultural regime at some critical Q (number of traits). Our results reveal a dependence of critical Q on the occupation probability p of persistent agents and we obtain the phase diagram of the model in the (p,Q) -plane. The critical locus is explained by the competition of two opposite forces named here barrier and bonding effects. Such forces are verified to be caused by non-persistent agents which adhere (adherent agents) to the set of traits of persistent ones. The adherence (concentration of adherent agents) as a function of p is found to decay for constant Q. Furthermore, adherence as a function of Q is found to decay as a power law with constant p.

Conceptualization, Development and Validation of an Instrument for Investigating Elements of Undergraduate Physics Laboratory Learning Environments: The UPLLES (Undergraduate Physics Laboratory Learning Environment Survey)

ERIC Educational Resources Information Center

Thomas, Gregory P; Meldrum, Al; Beamish, John

2013-01-01

First-year undergraduate physics laboratories are important physics learning environments. However, there is a lack of empirically informed literature regarding how students perceive their overall laboratory learning experiences. Recipe formats persist as the dominant form of instructional design in these sites, and these formats do not adequately…

Energy conversion modeling of the intrinsic persistent luminescence of solids via energy transfer paths between transition levels.

PubMed

Huang, Bolong; Sun, Mingzi

2017-04-05

An energy conversion model has been established for the intrinsic persistent luminescence in solids related to the native point defect levels, formations, and transitions. In this study, we showed how the recombination of charge carriers between different defect levels along the zero phonon line (ZPL) can lead to energy conversions supporting the intrinsic persistent phosphorescence in solids. This suggests that the key driving force for this optical phenomenon is the pair of electrons hopping between different charged defects with negative-U eff . Such a negative correlation energy will provide a sustainable energy source for electron-holes to further recombine in a new cycle with a specific quantum yield. This will help us to understand the intrinsic persistent luminescence with respect to native point defect levels as well as the correlations of electronics and energetics.

Breaking the cycle: extending the persistent pain cycle diagram using an affective pictorial metaphor.

PubMed

Stones, Catherine; Cole, Frances

2014-01-01

The persistent pain cycle diagram is a common feature of pain management literature. but how is it designed and is it fulfilling its potential in terms of providing information to motivate behavioral change? This article examines on-line persistent pain diagrams and critically discusses their purpose and design approach. By using broad information design theories by Karabeg and particular approaches to dialogic visual communications in business, this article argues the need for motivational as well as cognitive diagrams. It also outlines the design of a new persistent pain cycle that is currently being used with chronic pain patients in NHS Bradford, UK. This new cycle adopts and then visually extends an established verbal metaphor within acceptance and commitment therapy (ACT) in an attempt to increase the motivational aspects of the vicious circle diagram format.

Mobility-induced persistent chimera states

NASA Astrophysics Data System (ADS)

Petrungaro, Gabriela; Uriu, Koichiro; Morelli, Luis G.

2017-12-01

We study the dynamics of mobile, locally coupled identical oscillators in the presence of coupling delays. We find different kinds of chimera states in which coherent in-phase and antiphase domains coexist with incoherent domains. These chimera states are dynamic and can persist for long times for intermediate mobility values. We discuss the mechanisms leading to the formation of these chimera states in different mobility regimes. This finding could be relevant for natural and technological systems composed of mobile communicating agents.

Effect of Copper Oxide Concentration on the Formation and Persistency of Environmentally Persistent Free Radicals (EPFRs) in Particulates

PubMed Central

2015-01-01

Environmentally persistent free radicals (EPFRs) are formed by the chemisorption of substituted aromatics on metal oxide surfaces in both combustion sources and superfund sites. The current study reports the dependency of EPFR yields and their persistency on metal loading in particles (0.25, 0.5, 0.75, 1, 2, and 5% CuO/silica). The EPFRs were generated through exposure of particles to three adsorbate vapors at 230 °C: phenol, 2-monochlorophenol (2-MCP), and dichlorobenzene (DCBz). Adsorption resulted in the formation of surface-bound phenoxyl- and semiquinoine-type radicals with characteristic EPR spectra displaying a g value ranging from ∼2.0037 to 2.006. The highest EPFR yield was observed for CuO concentrations between 1 and 3% in relation to MCP and phenol adsorption. However, radical density, which is expressed as the number of radicals per copper atom, was highest at 0.75–1% CuO loading. For 1,2-dichlorobenzene adsorption, radical concentration increased linearly with decreasing copper content. At the same time, a qualitative change in the radicals formed was observed—from semiquinone to chlorophenoxyl radicals. The two longest lifetimes, 25 and 23 h, were observed for phenoxyl-type radicals on 0.5% CuO and chlorophenoxyl-type radicals on 0.75% CuO, respectively. PMID:24437381

Imbalanced Oxidative Stress Causes Chlamydial Persistence during Non-Productive Human Herpes Virus Co-Infection

PubMed Central

Prusty, Bhupesh K.; Böhme, Linda; Bergmann, Birgit; Siegl, Christine; Krause, Eva; Mehlitz, Adrian; Rudel, Thomas

2012-01-01

Both human herpes viruses and Chlamydia are highly prevalent in the human population and are detected together in different human disorders. Here, we demonstrate that co-infection with human herpes virus 6 (HHV6) interferes with the developmental cycle of C. trachomatis and induces persistence. Induction of chlamydial persistence by HHV6 is independent of productive virus infection, but requires the interaction and uptake of the virus by the host cell. On the other hand, viral uptake is strongly promoted under co-infection conditions. Host cell glutathione reductase activity was suppressed by HHV6 causing NADPH accumulation, decreased formation of reduced glutathione and increased oxidative stress. Prevention of oxidative stress restored infectivity of Chlamydia after HHV6-induced persistence. We show that co-infection with Herpes simplex virus 1 or human Cytomegalovirus also induces chlamydial persistence by a similar mechanism suggesting that Chlamydia -human herpes virus co-infections are evolutionary shaped interactions with a thus far unrecognized broad significance. PMID:23077614

New Weapons to Fight Old Enemies: Novel Strategies for the (Bio)control of Bacterial Biofilms in the Food Industry.

PubMed

Coughlan, Laura M; Cotter, Paul D; Hill, Colin; Alvarez-Ordóñez, Avelino

2016-01-01

Biofilms are microbial communities characterized by their adhesion to solid surfaces and the production of a matrix of exopolymeric substances, consisting of polysaccharides, proteins, DNA and lipids, which surround the microorganisms lending structural integrity and a unique biochemical profile to the biofilm. Biofilm formation enhances the ability of the producer/s to persist in a given environment. Pathogenic and spoilage bacterial species capable of forming biofilms are a significant problem for the healthcare and food industries, as their biofilm-forming ability protects them from common cleaning processes and allows them to remain in the environment post-sanitation. In the food industry, persistent bacteria colonize the inside of mixing tanks, vats and tubing, compromising food safety and quality. Strategies to overcome bacterial persistence through inhibition of biofilm formation or removal of mature biofilms are therefore necessary. Current biofilm control strategies employed in the food industry (cleaning and disinfection, material selection and surface preconditioning, plasma treatment, ultrasonication, etc.), although effective to a certain point, fall short of biofilm control. Efforts have been explored, mainly with a view to their application in pharmaceutical and healthcare settings, which focus on targeting molecular determinants regulating biofilm formation. Their application to the food industry would greatly aid efforts to eradicate undesirable bacteria from food processing environments and, ultimately, from food products. These approaches, in contrast to bactericidal approaches, exert less selective pressure which in turn would reduce the likelihood of resistance development. A particularly interesting strategy targets quorum sensing systems, which regulate gene expression in response to fluctuations in cell-population density governing essential cellular processes including biofilm formation. This review article discusses the problems associated with bacterial biofilms in the food industry and summarizes the recent strategies explored to inhibit biofilm formation, with special focus on those targeting quorum sensing.

Salmonella Extracellular Matrix Components Influence Biofilm Formation and Gallbladder Colonization.

PubMed

Adcox, Haley E; Vasicek, Erin M; Dwivedi, Varun; Hoang, Ky V; Turner, Joanne; Gunn, John S

2016-11-01

Salmonella enterica serovar Typhi, the causative agent of typhoid fever in humans, forms biofilms encapsulated by an extracellular matrix (ECM). Biofilms facilitate colonization and persistent infection in gallbladders of humans and mouse models of chronic carriage. Individual roles of matrix components have not been completely elucidated in vitro or in vivo To examine individual functions, strains of Salmonella enterica serovar Typhimurium, the murine model of S Typhi, in which various ECM genes were deleted or added, were created to examine biofilm formation, colonization, and persistence in the gallbladder. Studies show that curli contributes most significantly to biofilm formation. Expression of Vi antigen decreased biofilm formation in vitro and virulence and bacterial survival in vivo without altering the examined gallbladder pro- or anti-inflammatory cytokines. Oppositely, loss of all ECM components (ΔwcaM ΔcsgA ΔyihO ΔbcsE) increased virulence and bacterial survival in vivo and reduced gallbladder interleukin-10 (IL-10) levels. Colanic acid and curli mutants had the largest defects in biofilm-forming ability and contributed most significantly to the virulence increase of the ΔwcaM ΔcsgA ΔyihO ΔbcsE mutant strain. While the ΔwcaM ΔcsgA ΔyihO ΔbcsE mutant was not altered in resistance to complement or growth in macrophages, it attached and invaded macrophages better than the wild-type (WT) strain. These data suggest that ECM components have various levels of importance in biofilm formation and gallbladder colonization and that the ECM diminishes disseminated disease in our model, perhaps by reducing cell attachment/invasion and dampening inflammation by maintaining/inducing IL-10 production. Understanding how ECM components aid acute disease and persistence could lead to improvements in therapeutic treatment of typhoid fever patients. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Polarized and persistent Ca²⁺ plumes define loci for formation of wall ingrowth papillae in transfer cells.

PubMed

Zhang, Hui-Ming; Imtiaz, Mohammad S; Laver, Derek R; McCurdy, David W; Offler, Christina E; van Helden, Dirk F; Patrick, John W

2015-03-01

Transfer cell morphology is characterized by a polarized ingrowth wall comprising a uniform wall upon which wall ingrowth papillae develop at right angles into the cytoplasm. The hypothesis that positional information directing construction of wall ingrowth papillae is mediated by Ca(2+) signals generated by spatiotemporal alterations in cytosolic Ca(2+) ([Ca(2+)]cyt) of cells trans-differentiating to a transfer cell morphology was tested. This hypothesis was examined using Vicia faba cotyledons. On transferring cotyledons to culture, their adaxial epidermal cells synchronously trans-differentiate to epidermal transfer cells. A polarized and persistent Ca(2+) signal, generated during epidermal cell trans-differentiation, was found to co-localize with the site of ingrowth wall formation. Dampening Ca(2+) signal intensity, by withdrawing extracellular Ca(2+) or blocking Ca(2+) channel activity, inhibited formation of wall ingrowth papillae. Maintenance of Ca(2+) signal polarity and persistence depended upon a rapid turnover (minutes) of cytosolic Ca(2+) by co-operative functioning of plasma membrane Ca(2+)-permeable channels and Ca(2+)-ATPases. Viewed paradermally, and proximal to the cytosol-plasma membrane interface, the Ca(2+) signal was organized into discrete patches that aligned spatially with clusters of Ca(2+)-permeable channels. Mathematical modelling demonstrated that these patches of cytosolic Ca(2+) were consistent with inward-directed plumes of elevated [Ca(2+)]cyt. Plume formation depended upon an alternating distribution of Ca(2+)-permeable channels and Ca(2+)-ATPase clusters. On further inward diffusion, the Ca(2+) plumes coalesced into a uniform Ca(2+) signal. Blocking or dispersing the Ca(2+) plumes inhibited deposition of wall ingrowth papillae, while uniform wall formation remained unaltered. A working model envisages that cytosolic Ca(2+) plumes define the loci at which wall ingrowth papillae are deposited. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

New Weapons to Fight Old Enemies: Novel Strategies for the (Bio)control of Bacterial Biofilms in the Food Industry

PubMed Central

Coughlan, Laura M.; Cotter, Paul D.; Hill, Colin; Alvarez-Ordóñez, Avelino

2016-01-01

Biofilms are microbial communities characterized by their adhesion to solid surfaces and the production of a matrix of exopolymeric substances, consisting of polysaccharides, proteins, DNA and lipids, which surround the microorganisms lending structural integrity and a unique biochemical profile to the biofilm. Biofilm formation enhances the ability of the producer/s to persist in a given environment. Pathogenic and spoilage bacterial species capable of forming biofilms are a significant problem for the healthcare and food industries, as their biofilm-forming ability protects them from common cleaning processes and allows them to remain in the environment post-sanitation. In the food industry, persistent bacteria colonize the inside of mixing tanks, vats and tubing, compromising food safety and quality. Strategies to overcome bacterial persistence through inhibition of biofilm formation or removal of mature biofilms are therefore necessary. Current biofilm control strategies employed in the food industry (cleaning and disinfection, material selection and surface preconditioning, plasma treatment, ultrasonication, etc.), although effective to a certain point, fall short of biofilm control. Efforts have been explored, mainly with a view to their application in pharmaceutical and healthcare settings, which focus on targeting molecular determinants regulating biofilm formation. Their application to the food industry would greatly aid efforts to eradicate undesirable bacteria from food processing environments and, ultimately, from food products. These approaches, in contrast to bactericidal approaches, exert less selective pressure which in turn would reduce the likelihood of resistance development. A particularly interesting strategy targets quorum sensing systems, which regulate gene expression in response to fluctuations in cell-population density governing essential cellular processes including biofilm formation. This review article discusses the problems associated with bacterial biofilms in the food industry and summarizes the recent strategies explored to inhibit biofilm formation, with special focus on those targeting quorum sensing. PMID:27803696

[Computer tomography in the diagnosis of persistent hyperplastic primary vitreous body].

PubMed

Prokes, B; Rehůrek, J

1989-10-01

The authors described and evaluated clinical and CT pictures of five children with persistence of hyperplastic primary vitreous body originating due to regression of embryonal hyaloid vascular system. It becomes clinically manifest especially in leucocoria, reduced globe of the eye, prolonged ciliary processi and the formation of fibrovascular changes behind the lens. CT picture is characterized by a) increased density of vitreous body, b) dense stripes going in retrolental direction and in the course of the Cloquet canal, c) microphthalmus, d) absence of calcifications and e) facultative changes on the lens and anterior chamber. These signs represent an important criterium for differentiating persistence of hyperplastic primary vitreous body from retinoblastoma.

Evidence That Nitric Acid Increases Relative Humidity in Low-Temperature Cirrus Clouds

NASA Technical Reports Server (NTRS)

Gao, R. S.; Popp, P. J.; Fahey, D. W.; Marcy, T. P.; Herman, R. L.; Weinstock, E. M.; Baumgardner, D. G.; Garrett, T. J.; Rosenlof, K. H.; Thompson, T. L.

2004-01-01

In situ measurements of the relative humidity with respect to ice (RH(sub(i)) and of nitric acid (HNO3) were made in both natural and contrail cirrus clouds in the upper troposphere. At temperatures lower than 202 kelvin, RH(sub i) values show a sharp increase to average values of over 130% in both cloud types. These enhanced RH(sub i) values are attributed to the presence of a new class of NHO3- containing ice particles (Delta-ice). We propose that surface HNO3 molecules prevent the ice/vapor system from reaching equilibrium by a mechanism similar to that of freezing point depression by antifreeze proteins. Delta-ice represents a new link between global climate and natural and anthropogenic nitrogen oxide emissions. Including Delta-ice in climate models will alter simulated cirrus properties and the distribution of upper tropospheric water vapor.

Examination of diurnal temperature range at coterminous U.S. stations during Sept. 8-17, 2001

NASA Astrophysics Data System (ADS)

van Wijngaarden, W. A.

2012-07-01

The tragic events of Sept. 11, 2001 resulted in suspension of commercial flights over North America. It has been suggested that the diurnal temperature range (DTR) increased due to an absence of airplane contrails. This study examined hourly data observed at 288 stations. The average DTR, temperature, maximum/minimum temperature and relative humidity were found for each day in 2001 and compared to the average value occurring during 1975-2005. For the coterminous U.S., the DTR averaged over the period Sept. 11-14, 2001 was about 1°C larger than that found for the 3 days prior and after the flight ban. However, the day-to-day DTR does not correlate well with the flight ban. Plots of the change in DTR throughout North America during Sept. 8-17 show changes consistent with the natural progression of weather systems.

Hypercalibration: A Pan-STARRS1-Based Recalibration of the Sloan Digital Sky Survey Photometry

DOE PAGES

Finkbeiner, Douglas P.; Schlafly, Edward F.; Schlegel, David J.; ...

2016-05-05

In this paper, we present a recalibration of the Sloan Digital Sky Survey (SDSS) photometry with new flat fields and zero points derived from Pan-STARRS1. Using point-spread function (PSF) photometry of 60 million stars with 16 < r < 20, we derive a model of amplifier gain and flat-field corrections with per-run rms residuals of 3 millimagnitudes (mmag) in griz bands and 15 mmag in u band. The new photometric zero points are adjusted to leave the median in the Galactic north unchanged for compatibility with previous SDSS work. We also identify transient non-photometric periods in SDSS ("contrails") based onmore » photometric deviations co-temporal in SDSS bands. Finally, the recalibrated stellar PSF photometry of SDSS and PS1 has an rms difference of {9, 7, 7, 8} mmag in griz, respectively, when averaged over 15' regions.« less

Formational Turning Points in the Transition to College: Understanding How Communication Events Shape First-Generation Students' Pedagogical and Interpersonal Relationships with Their College Teachers

ERIC Educational Resources Information Center

Wang, Tiffany R.

2014-01-01

In the present study, I explored student-teacher interaction, student-teacher relationship formation and development, and the ways in which student-teacher interaction and relationships facilitated support and persistence for first-generation (FG) students during the transition to college. Using transition theory as a sensitizing framework, I took…

Persistence Increases in the Absence of the Alarmone Guanosine Tetraphosphate by Reducing Cell Growth

DTIC Science & Technology

2016-08-16

this persister state is guanosine tetraphosphate (ppGpp), the alarmone that was first linked to nutrient stress. In Escherichia coli , ppGpp redirects...on guanosine tetraphosphate (ppGpp) and on toxins of toxin/ antitoxin (TA) systems. In Escherichia coli , ppGpp is produced as a response to nutrient...formation by inducing the TisB toxin in Escherichia coli . PLoS Biol. 8, e1000317 (2010). 8. Hu, Y., Kwan, B. W., Osbourne, D. O., Benedik, M. J

Evidence for persistent flow and aqueous sedimentation on early Mars.

PubMed

Malin, Michael C; Edgett, Kenneth S

2003-12-12

Landforms representative of sedimentary processes and environments that occurred early in martian history have been recognized in Mars Global Surveyor Mars Orbiter Camera and Mars Odyssey Thermal Emission Imaging System images. Evidence of distributary, channelized flow (in particular, flow that lasted long enough to foster meandering) and the resulting deposition of a fan-shaped apron of debris indicate persistent flow conditions and formation of at least some large intracrater layered sedimentary sequences within fluvial, and potentially lacustrine, environments.

Organic Nanocrystals with Bright Red Persistent Room-Temperature Phosphorescence for Biological Applications.

PubMed

Fateminia, S M Ali; Mao, Zhu; Xu, Shidang; Yang, Zhiyong; Chi, Zhenguo; Liu, Bin

2017-09-25

Persistent room-temperature phosphorescence (RTP) in pure organic materials has attracted great attention because of their unique optical properties. The design of organic materials with bright red persistent RTP remains challenging. Herein, we report a new design strategy for realizing high brightness and long lifetime of red-emissive RTP molecules, which is based on introducing an alkoxy spacer between the hybrid units in the molecule. The spacer offers easy Br-H bond formation during crystallization, which also facilitates intermolecular electron coupling to favor persistent RTP. As the majority of RTP compounds have to be confined in a rigid environment to quench nonradiative relaxation pathways for bright phosphorescence emission, nanocrystallization is used to not only rigidify the molecules but also offer the desirable size and water-dispersity for biomedical applications. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

comK Prophage Junction Fragments as Markers for Listeria monocytogenes Genotypes Unique to Individual Meat and Poultry Processing Plants and a Model for Rapid Niche-Specific Adaptation, Biofilm Formation, and Persistence ▿ †

PubMed Central

Verghese, Bindhu; Lok, Mei; Wen, Jia; Alessandria, Valentina; Chen, Yi; Kathariou, Sophia; Knabel, Stephen

2011-01-01

Different strains of Listeria monocytogenes are well known to persist in individual food processing plants and to contaminate foods for many years; however, the specific genotypic and phenotypic mechanisms responsible for persistence of these unique strains remain largely unknown. Based on sequences in comK prophage junction fragments, different strains of epidemic clones (ECs), which included ECII, ECIII, and ECV, were identified and shown to be specific to individual meat and poultry processing plants. The comK prophage-containing strains showed significantly higher cell densities after incubation at 30°C for 48 h on meat and poultry food-conditioning films than did strains lacking the comK prophage (P < 0.05). Overall, the type of strain, the type of conditioning film, and the interaction between the two were all highly significant (P < 0.001). Recombination analysis indicated that the comK prophage junction fragments in these strains had evolved due to extensive recombination. Based on the results of the present study, we propose a novel model in which the concept of defective comK prophage was replaced with the rapid adaptation island (RAI). Genes within the RAI were recharacterized as “adaptons,” as these genes may allow L. monocytogenes to rapidly adapt to different food processing facilities and foods. If confirmed, the model presented would help explain Listeria's rapid niche adaptation, biofilm formation, persistence, and subsequent transmission to foods. Also, comK prophage junction fragment sequences may permit accurate tracking of persistent strains back to and within individual food processing operations and thus allow the design of more effective intervention strategies to reduce contamination and enhance food safety. PMID:21441318

comK prophage junction fragments as markers for Listeria monocytogenes genotypes unique to individual meat and poultry processing plants and a model for rapid niche-specific adaptation, biofilm formation, and persistence.

PubMed

Verghese, Bindhu; Lok, Mei; Wen, Jia; Alessandria, Valentina; Chen, Yi; Kathariou, Sophia; Knabel, Stephen

2011-05-01

Different strains of Listeria monocytogenes are well known to persist in individual food processing plants and to contaminate foods for many years; however, the specific genotypic and phenotypic mechanisms responsible for persistence of these unique strains remain largely unknown. Based on sequences in comK prophage junction fragments, different strains of epidemic clones (ECs), which included ECII, ECIII, and ECV, were identified and shown to be specific to individual meat and poultry processing plants. The comK prophage-containing strains showed significantly higher cell densities after incubation at 30°C for 48 h on meat and poultry food-conditioning films than did strains lacking the comK prophage (P < 0.05). Overall, the type of strain, the type of conditioning film, and the interaction between the two were all highly significant (P < 0.001). Recombination analysis indicated that the comK prophage junction fragments in these strains had evolved due to extensive recombination. Based on the results of the present study, we propose a novel model in which the concept of defective comK prophage was replaced with the rapid adaptation island (RAI). Genes within the RAI were recharacterized as "adaptons," as these genes may allow L. monocytogenes to rapidly adapt to different food processing facilities and foods. If confirmed, the model presented would help explain Listeria's rapid niche adaptation, biofilm formation, persistence, and subsequent transmission to foods. Also, comK prophage junction fragment sequences may permit accurate tracking of persistent strains back to and within individual food processing operations and thus allow the design of more effective intervention strategies to reduce contamination and enhance food safety.

Environmentally Persistent Free Radical (EPFRs) - Ambient Air Particulates, Soils and Fate of Some Pollutants

NASA Astrophysics Data System (ADS)

Lomnicki, S. M.

2017-12-01

Environmentally Persistent Free Radicals (EPFRs) are relatively recently discovered species that are present on ambient air particulates. Their origin is typically associated with the combustion borne PM, where in the cool zone of the combustion process aromatic precursors react with the metal centers of particulates forming surface-organic complex with radical characteristics. EPFRs have been found to be sufficiently resistant to be emitted from the combustion sources and persist in the ambient air on particulates. Their inhalation has been associated with severe health effects, and potentially are one of the major agents contributing the epidemiological risks of PM exposure. Interestingly, EPFRs can be formed not only at the elevated temperatures but also in ambient conditions, where the contact of precursor molecules with transition metal (but not only) domains can result in adsorbate complexes. In fact, EPFRs have been detected in the contaminated soils, or during the oil spill incidents. It is very likely, that the interaction of some molecules released to the air can result in the formation of EPFRs on the ambient air particulates in atmospheric conditions. These species can be a natural degradation by-products that lead to the formation of oxygenated organics in ambient atmosphere.

Dynamic Sumoylation of a Conserved Transcription Corepressor Prevents Persistent Inclusion Formation during Hyperosmotic Stress

PubMed Central

Oeser, Michelle L.; Amen, Triana; Nadel, Cory M.; Bradley, Amanda I.; Reed, Benjamin J.; Jones, Ramon D.; Gopalan, Janani; Kaganovich, Daniel; Gardner, Richard G.

2016-01-01

Cells are often exposed to physical or chemical stresses that can damage the structures of essential biomolecules. Stress-induced cellular damage can become deleterious if not managed appropriately. Rapid and adaptive responses to stresses are therefore crucial for cell survival. In eukaryotic cells, different stresses trigger post-translational modification of proteins with the small ubiquitin-like modifier SUMO. However, the specific regulatory roles of sumoylation in each stress response are not well understood. Here, we examined the sumoylation events that occur in budding yeast after exposure to hyperosmotic stress. We discovered by proteomic and biochemical analyses that hyperosmotic stress incurs the rapid and transient sumoylation of Cyc8 and Tup1, which together form a conserved transcription corepressor complex that regulates hundreds of genes. Gene expression and cell biological analyses revealed that sumoylation of each protein directs distinct outcomes. In particular, we discovered that Cyc8 sumoylation prevents the persistence of hyperosmotic stress-induced Cyc8-Tup1 inclusions, which involves a glutamine-rich prion domain in Cyc8. We propose that sumoylation protects against persistent inclusion formation during hyperosmotic stress, allowing optimal transcriptional function of the Cyc8-Tup1 complex. PMID:26800527

Increased mitochondrial functions in human glioblastoma cells persistently infected with measles virus.

PubMed

Takahashi, Megumi; Wolf, Alexander M; Watari, Eiji; Norose, Yoshihiko; Ohta, Shigeo; Takahashi, Hidemi

2013-09-01

Measles virus (MV) is known for its ability to cause an acute infection with a potential of development of persistent infection. However, knowledge of how viral genes and cellular factors interact to cause or maintain the persistent infection has remained unclear. We have previously reported the possible involvement of mitochondrial short chain enoyl-CoA hydratase (ECHS), which is localized at mitochondria, in the regulation of MV replication. In this study we found increased functions of mitochondria in MV-persistently infected cells compared with uninfected or acutely infected cells. Furthermore, impairment of mitochondrial functions by treatment with mitochondrial inhibitors such as ethidium bromide (EtBr) or carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) induced the cytopathic effects of extensive syncytial formation in persistently infected cells. These findings suggest that mitochondria are one of the subcellular organelles contributing to regulate persistent infection of MV. Recent studies showed mitochondria provide an integral platform for retinoic acid-inducible protein (RIG-I)-like cytosolic receptors (RLRs) signaling and participate in cellular innate antiviral immunity. Our findings not only reveal a role of mitochondria in RLR mediated antiviral signaling but also suggest that mitochondria contribute to the regulation of persistent viral infection. Copyright © 2013 Elsevier B.V. All rights reserved.

Cyclic AMP Regulates Bacterial Persistence through Repression of the Oxidative Stress Response and SOS-Dependent DNA Repair in Uropathogenic Escherichia coli.

PubMed

Molina-Quiroz, Roberto C; Silva-Valenzuela, Cecilia; Brewster, Jennifer; Castro-Nallar, Eduardo; Levy, Stuart B; Camilli, Andrew

2018-01-09

Bacterial persistence is a transient, nonheritable physiological state that provides tolerance to bactericidal antibiotics. The stringent response, toxin-antitoxin modules, and stochastic processes, among other mechanisms, play roles in this phenomenon. How persistence is regulated is relatively ill defined. Here we show that cyclic AMP, a global regulator of carbon catabolism and other core processes, is a negative regulator of bacterial persistence in uropathogenic Escherichia coli , as measured by survival after exposure to a β-lactam antibiotic. This phenotype is regulated by a set of genes leading to an oxidative stress response and SOS-dependent DNA repair. Thus, persister cells tolerant to cell wall-acting antibiotics must cope with oxidative stress and DNA damage and these processes are regulated by cyclic AMP in uropathogenic E. coli IMPORTANCE Bacterial persister cells are important in relapsing infections in patients treated with antibiotics and also in the emergence of antibiotic resistance. Our results show that in uropathogenic E. coli , the second messenger cyclic AMP negatively regulates persister cell formation, since in its absence much more persister cells form that are tolerant to β-lactams antibiotics. We reveal the mechanism to be decreased levels of reactive oxygen species, specifically hydroxyl radicals, and SOS-dependent DNA repair. Our findings suggest that the oxidative stress response and DNA repair are relevant pathways to target in the design of persister-specific antibiotic compounds. Copyright © 2018 Molina-Quiroz et al.

Post-Deposition (and Ongoing?) Modification of Caloris Ejecta Blocks

NASA Astrophysics Data System (ADS)

Wright, J.; Conway, S. J.; Balme, M. R.; Rothery, D. A.

2018-05-01

Caloris ejecta blocks have been modified by mass-wasting that has persisted long after their formation. Volatiles may be involved in this process. Block geomorphology therefore has implications for Mercury's interior volatile content.

A dityrosine network mediated by dual oxidase and peroxidase influences the persistence of Lyme disease pathogens within the vector.

PubMed

Yang, Xiuli; Smith, Alexis A; Williams, Mark S; Pal, Utpal

2014-05-02

Ixodes scapularis ticks transmit a wide array of human and animal pathogens including Borrelia burgdorferi; however, how tick immune components influence the persistence of invading pathogens remains unknown. As originally demonstrated in Caenorhabditis elegans and later in Anopheles gambiae, we show here that an acellular gut barrier, resulting from the tyrosine cross-linking of the extracellular matrix, also exists in I. scapularis ticks. This dityrosine network (DTN) is dependent upon a dual oxidase (Duox), which is a member of the NADPH oxidase family. The Ixodes genome encodes for a single Duox and at least 16 potential peroxidase proteins, one of which, annotated as ISCW017368, together with Duox has been found to be indispensible for DTN formation. This barrier influences pathogen survival in the gut, as an impaired DTN in Doux knockdown or in specific peroxidase knockdown ticks, results in reduced levels of B. burgdorferi persistence within ticks. Absence of a complete DTN formation in knockdown ticks leads to the activation of specific tick innate immune pathway genes that potentially resulted in the reduction of spirochete levels. Together, these results highlighted the evolution of the DTN in a diverse set of arthropod vectors, including ticks, and its role in protecting invading pathogens like B. burgdorferi. Further understanding of the molecular basis of tick innate immune responses, vector-pathogen interaction, and their contributions in microbial persistence may help the development of new targets for disrupting the pathogen life cycle.

Representativeness analysis of CO_{2} profiles near a tall tower and from commercial airliner programs

NASA Astrophysics Data System (ADS)

Chen, Huilin; Katrynski, Krzysztof; Nedelec, Philippe; Machida, Toshinobu; Matsueda, Hidekazu; Sawa, Yousuke; Gerbig, Christoph

2010-05-01

Aircraft profiles for atmospheric trace gases have been collected using both rental aircraft and from commercial airliners. High-accuracy regular in situ CO2 measurements aboard rental aircraft over northeast Poland have been upgraded since August 2008. During each flight, two profiles are taken with a spatial separation of 20 kilometers. Until now, 74 profiles with continuous CO2 have been collected. Meanwhile, aircraft profiles for carbon monoxide (CO) have been made aboard commercial airliners within MOZAIC (Measurement of Ozone, water vapor, carbon monoxide and nitrogen oxides by AIrbus in-service airCraft) and for CO2 within CONTRAIL (Comprehensive Observation Network for TRace gases byAIrLiner) respectively. Starting from 2011, IAGOS-ERI (Integration of routine Aircraft measurements into a Global Observing System - European Research Infrastructure) will provide continuous CO2, CH4 and H2O measurements using instruments deployed aboard commercial airliners, with many profiles during take-off and landing over airports distributed all over the globe. These profiles contain not only vertical gradients but also regionally representative information. It is of importance to investigate how these profiles could be used for applications such as satellite validation and inverse modeling to retrieve surface-atmosphere exchange fluxes of greenhouse gases at regional to continental scales. Especially profiles from commercial airliners near major cities, which are potentially influenced by local fossil fuel emissions, need to be assessed with respect to their regional representativeness. We analyzed CO profiles over Frankfurt airport from the MOZAIC and CO2 profiles from CONTRAIL using STILT (the Stochastic Time Inverted Lagrangian Transport model) combined with a high resolution CO emission map in central Europe. Combining STILT footprints (maps of sensitivities to upstream surface fluxes) with high resolution emission inventories allows to attribute the contribution fossil fuel emissions to local vs. regional sources. In contrast, we analyzed CO2 profiles over northeast Poland in a similar way, where fossil fuel emissions are insignificant. The representativeness analysis provides information on under which circumstances such profiles can be used for potential applications, i.e. satellite validation and inverse modeling. The analysis suggests that a combined measurement of CO2 and CO significantly improves the usability of the regular profiles, where CO serves as the emission tracer.

Specific Human and Candida Cellular Interactions Lead to Controlled or Persistent Infection Outcomes during Granuloma-Like Formation.

PubMed

Misme-Aucouturier, Barbara; Albassier, Marjorie; Alvarez-Rueda, Nidia; Le Pape, Patrice

2017-01-01

A delayed type of multicellular process could be crucial during chronic candidiasis in determining the course of infection. This reaction, consisting of organized immune cells surrounding the pathogen, initiates an inflammatory response to avoid fungal dissemination. The goal of the present study was to examine, at an in vitro cellular scale, Candida and human immune cell interaction dynamics during a long-term period. By challenging human peripheral blood immune cells from 10 healthy donors with 32 Candida albicans and non-albicans (C. glabrata, C. tropicalis, C. parapsilosis, C. dubliniensis, C. lusitaniae, C. krusei, and C. kefyr) clinical isolates, we showed that Candida spp. induced the formation of granuloma-like structures within 6 days after challenge, but their sizes and the respective fungal burdens differed according to the Candida species. These two parameters are positively correlated. Phenotypic characteristics, such as hypha formation and higher axenic growth rate, seem to contribute to yeast persistence within granuloma-like structures. We showed an interindividual variability of the human response against Candida spp. Higher proportions of neutrophils and elevated CD4 + /CD8 + T cell ratios during the first days after challenge were correlated with early production of gamma interferon (IFN-γ) and associated with controlled infection. In contrast, the persistence of Candida could result from upregulation of proinflammatory cytokines such as interleukin-6 (IL-6), IFN-γ, and tumor necrosis factor alpha (TNF-α) and a poor anti-inflammatory negative feedback (IL-10). Importantly, regulatory subsets of NK cells and CD4 lo CD8 hi doubly positive (DP) lymphocytes at late stage infiltrate granuloma-like structures and could correlate with the IL-10 and TNF-α production. These data offer a base frame to explain cellular events that guide infection control or fungal persistence. Copyright © 2016 Misme-Aucouturier et al.

Impact of Medium and Substrate on Growth of Pseudomonas Fluorescens Biofilms on Polyurethane Paint

DTIC Science & Technology

2011-02-01

biofilm formation on polyurethane (PU) coatings, and to define how those parameters contribute to polyurethane biodegradation. We used a batch flow system...determine which factors best support the growth and persistence of Pseudomonas fluorescens biofilms . Factors that enhance biofilm formation and...AFRL-RX-WP-TP-2011-4131 IMPACT OF MEDIUM AND SUBSTRATE ON GROWTH OF PSEUDOMONAS FLUORESCENS BIOFILMS ON POLYURETHANE PAINT Wendy L. Goodson

Additive Effects of Mechanical Marrow Ablation and PTH Treatment on de Novo Bone Formation in Mature Adult Rats

PubMed Central

Zhang, Qing; Miller, Christopher; Bible, Jesse; Li, Jiliang; Xu, Xiaoqing; Mehta, Nozer; Gilligan, James; Vignery, Agnès; Scholz, Jodi A Carlson

2012-01-01

Mechanical ablation of bone marrow in young rats induces rapid but transient bone growth, which can be enhanced and maintained for three weeks by the administration of parathyroid hormone (PTH). Additionally, marrow ablation, followed by PTH treatment for three months leads to increased cortical thickness. In this study, we sought to determine whether PTH enhances bone formation after marrow ablation in aged rats. Aged rats underwent unilateral femoral marrow ablation and treatment with PTH or vehicle for four weeks. Both femurs from each rat were analyzed by X-ray and pQCT, then analyzed either by microCT, histology or biomechanical testing. Marrow ablation alone induced transient bone formation of low abundance that persisted over four weeks, while marrow ablation followed by PTH induced bone formation of high abundance that also persisted over four weeks. Our data confirms that the osteo-inducive effect of marrow ablation and the additive effect of marrow ablation, followed by PTH, occurs in aged rats. Our observations open new avenues of investigations in the field of tissue regeneration. Local marrow ablation, in conjunction with an anabolic agent, might provide a new platform for rapid site-directed bone growth in areas of high bone loss, such as in the hip and wrist, which are subject to fracture. PMID:24710549

Cloud condensation nuclei and ice nucleation activity of hydrophobic and hydrophilic soot particles.

PubMed

Koehler, Kirsten A; DeMott, Paul J; Kreidenweis, Sonia M; Popovicheva, Olga B; Petters, Markus D; Carrico, Christian M; Kireeva, Elena D; Khokhlova, Tatiana D; Shonija, Natalia K

2009-09-28

Cloud condensation nuclei (CCN) activity and ice nucleation behavior (for temperatures

Biofilm Formation Potential of Heat-Resistant Escherichia coli Dairy Isolates and the Complete Genome of Multidrug-Resistant, Heat-Resistant Strain FAM21845

PubMed Central

Schmid, Michael; Kulli, Sandra; Schneeberger, Kerstin; Naskova, Javorka; Knøchel, Susanne; Ahrens, Christian H.

2017-01-01

ABSTRACT We tested the biofilm formation potential of 30 heat-resistant and 6 heat-sensitive Escherichia coli dairy isolates. Production of curli and cellulose, static biofilm formation on polystyrene (PS) and stainless steel surfaces, biofilm formation under dynamic conditions (Bioflux), and initial adhesion rates (IAR) were evaluated. Biofilm formation varied greatly between strains, media, and assays. Our results highlight the importance of the experimental setup in determining biofilm formation under conditions of interest, as correlation between different assays was often not a given. The heat-resistant, multidrug-resistant (MDR) strain FAM21845 showed the strongest biofilm formation on PS and the highest IAR and was the only strain that formed significant biofilms on stainless steel under conditions relevant to the dairy industry, and it was therefore fully sequenced. Its chromosome is 4.9 Mb long, and it harbors a total of five plasmids (147.2, 54.2, 5.8, 2.5, and 1.9 kb). The strain carries a broad range of genes relevant to antimicrobial resistance and biofilm formation, including some on its two large conjugative plasmids, as demonstrated in plate mating assays. IMPORTANCE In biofilms, cells are embedded in an extracellular matrix that protects them from stresses, such as UV radiation, osmotic shock, desiccation, antibiotics, and predation. Biofilm formation is a major bacterial persistence factor of great concern in the clinic and the food industry. Many tested strains formed strong biofilms, and especially strains such as the heat-resistant, MDR strain FAM21845 may pose a serious issue for food production. Strong biofilm formation combined with diverse resistances (some encoded on conjugative plasmids) may allow for increased persistence, coselection, and possible transfer of these resistance factors. Horizontal gene transfer may conceivably occur in the food production setting or the gastrointestinal tract after consumption. PMID:28550056

Biofilm Formation Potential of Heat-Resistant Escherichia coli Dairy Isolates and the Complete Genome of Multidrug-Resistant, Heat-Resistant Strain FAM21845.

PubMed

Marti, Roger; Schmid, Michael; Kulli, Sandra; Schneeberger, Kerstin; Naskova, Javorka; Knøchel, Susanne; Ahrens, Christian H; Hummerjohann, Jörg

2017-08-01

We tested the biofilm formation potential of 30 heat-resistant and 6 heat-sensitive Escherichia coli dairy isolates. Production of curli and cellulose, static biofilm formation on polystyrene (PS) and stainless steel surfaces, biofilm formation under dynamic conditions (Bioflux), and initial adhesion rates (IAR) were evaluated. Biofilm formation varied greatly between strains, media, and assays. Our results highlight the importance of the experimental setup in determining biofilm formation under conditions of interest, as correlation between different assays was often not a given. The heat-resistant, multidrug-resistant (MDR) strain FAM21845 showed the strongest biofilm formation on PS and the highest IAR and was the only strain that formed significant biofilms on stainless steel under conditions relevant to the dairy industry, and it was therefore fully sequenced. Its chromosome is 4.9 Mb long, and it harbors a total of five plasmids (147.2, 54.2, 5.8, 2.5, and 1.9 kb). The strain carries a broad range of genes relevant to antimicrobial resistance and biofilm formation, including some on its two large conjugative plasmids, as demonstrated in plate mating assays. IMPORTANCE In biofilms, cells are embedded in an extracellular matrix that protects them from stresses, such as UV radiation, osmotic shock, desiccation, antibiotics, and predation. Biofilm formation is a major bacterial persistence factor of great concern in the clinic and the food industry. Many tested strains formed strong biofilms, and especially strains such as the heat-resistant, MDR strain FAM21845 may pose a serious issue for food production. Strong biofilm formation combined with diverse resistances (some encoded on conjugative plasmids) may allow for increased persistence, coselection, and possible transfer of these resistance factors. Horizontal gene transfer may conceivably occur in the food production setting or the gastrointestinal tract after consumption. Copyright © 2017 Marti et al.

Tight coupling between nucleus and cell migration through the perinuclear actin cap

PubMed Central

Kim, Dong-Hwee; Cho, Sangkyun; Wirtz, Denis

2014-01-01

ABSTRACT Although eukaryotic cells are known to alternate between ‘advancing’ episodes of fast and persistent movement and ‘hesitation’ episodes of low speed and low persistence, the molecular mechanism that controls the dynamic changes in morphology, speed and persistence of eukaryotic migratory cells remains unclear. Here, we show that the movement of the interphase nucleus during random cell migration switches intermittently between two distinct modes – rotation and translocation – that follow with high fidelity the sequential rounded and elongated morphologies of the nucleus and cell body, respectively. Nuclear rotation and translocation mediate the stop-and-go motion of the cell through the dynamic formation and dissolution, respectively, of the contractile perinuclear actin cap, which is dynamically coupled to the nuclear lamina and the nuclear envelope through LINC complexes. A persistent cell movement and nuclear translocation driven by the actin cap are halted following the disruption of the actin cap, which in turn allows the cell to repolarize for its next persistent move owing to nuclear rotation mediated by cytoplasmic dynein light intermediate chain 2. PMID:24639463

Small protein-mediated quorum sensing in a gram-negative bacterium: novel targets for control of infectious disease.

PubMed

Ronald, Pamela C

2011-12-01

Control of Gram-negative bacterial infections of plants and animals remains a major challenge because conventional approaches are often not sufficient to eradicate these infections. One major reason for their persistence seems to be the capability of the bacteria to grow within biofilms that protect them from adverse environmental factors. Quorum sensing (QS) plays an important role in the formation of biofilms. In QS, small molecules serve as signals to recognize bacterial cell population size, leading to changes in expression of specific genes when a signal has accumulated to some threshold concentration. The small protein Ax21 (Activator of XA21-mediated immunity), serves as a QS factor that regulates biofilm formation and virulence in the Gram-negative bacterium, Xanthomonas oryzae pv. oryzae. Knowledge of small protein-mediated QS in Gram-negative bacteria can be used to develop new methods to control persistent Gram-negative infections. © Discovery Medicine

The continuous and persistent periodical growth induced by substrate accommodation in In2O3 nanostructure chains and their photoluminescence properties

NASA Astrophysics Data System (ADS)

Shariati, Mohsen

2015-03-01

The growth of pyramidal and triangular beaded In2O3 nanocrystal chains by using oxygen-assisted thermal evaporation, substrate accommodation and condensation method has been articulated. Self-assembled In2O3 nanocrystal chains have been synthesized by the vapor-solid (VS) and vapor-liquid-solid (VLS) growth mechanism and also through controlling the kinetics factors (saturation ratio). A periodical one-dimensional (1-D) and persistent (0-D) growth was proposed to explain the formation of lateral nanostructures, and this formation aspect was ascribed to the alternate 1-D and 0-D growth. Preparing the needed growth factor, the In2O3 nanocrystal chains extended to several micrometers. The growth mechanism analysis was useful to realize the relation between the kinetics factors and the complex nanostructure. The morphology and size of nanocrystals intensively were changed by oxygen concentration and led to interesting photoluminescence property.

Mycobacteria exploit nitric oxide-induced transformation of macrophages into permissive giant cells.

PubMed

Gharun, Kourosh; Senges, Julia; Seidl, Maximilian; Lösslein, Anne; Kolter, Julia; Lohrmann, Florens; Fliegauf, Manfred; Elgizouli, Magdeldin; Vavra, Martina; Schachtrup, Kristina; Illert, Anna L; Gilleron, Martine; Kirschning, Carsten J; Triantafyllopoulou, Antigoni; Henneke, Philipp

2017-12-01

Immunity to mycobacteria involves the formation of granulomas, characterized by a unique macrophage (MΦ) species, so-called multinucleated giant cells (MGC). It remains unresolved whether MGC are beneficial to the host, that is, by prevention of bacterial spread, or whether they promote mycobacterial persistence. Here, we show that the prototypical antimycobacterial molecule nitric oxide (NO), which is produced by MGC in excessive amounts, is a double-edged sword. Next to its antibacterial capacity, NO propagates the transformation of MΦ into MGC, which are relatively permissive for mycobacterial persistence. The mechanism underlying MGC formation involves NO-induced DNA damage and impairment of p53 function. Moreover, MGC have an unsurpassed potential to engulf mycobacteria-infected apoptotic cells, which adds a further burden to their antimycobacterial capacity. Accordingly, mycobacteria take paradoxical advantage of antimicrobial cellular efforts by driving effector MΦ into a permissive MGC state. © 2017 The Authors.

[Formation and persistence of L-variants of Salmonella typhi in experimental typhoid and in carriers].

PubMed

Levina, G A; Prozorovskiĭ, S V; Iagud, S L; Grumman, M I; Gorelov, A L

1981-07-01

The possibility of the induction and persistence of S. typhi L-forms in the process of experimental typhoid infection and carriership has been studied in rabbits. This study has revealed that the process of L-transformation leading to the appearance of the imbalanced growth forms and unstable L-forms of S. typhi in the organism of the animals infected with S. typhi culture may occur under the conditions of carriership. Such changed forms can be detected in the organism of the animals 18 months after the primary infection.

Stress within a Restricted Time Window Selectively Affects the Persistence of Long-Term Memory

PubMed Central

Fang, Qin; Chai, Ning; Zhao, Li-Yan; Xue, Yan-Xue; Luo, Yi-Xiao; Jian, Min; Han, Ying; Shi, Hai-Shui; Lu, Lin; Wu, Ping; Wang, Ji-Shi

2013-01-01

The effects of stress on emotional memory are distinct and depend on the stages of memory. Memory undergoes consolidation and reconsolidation after acquisition and retrieval, respectively. Stress facilitates the consolidation but disrupts the reconsolidation of emotional memory. Previous research on the effects of stress on memory have focused on long-term memory (LTM) formation (tested 24 h later), but the effects of stress on the persistence of LTM (tested at least 1 week later) are unclear. Recent findings indicated that the persistence of LTM requires late-phase protein synthesis in the dorsal hippocampus. The present study investigated the effect of stress (i.e., cold water stress) during the late phase after the acquisition and retrieval of contextual fear memory in rats. We found that stress and corticosterone administration during the late phase (12 h) after acquisition, referred to as late consolidation, selectively enhanced the persistence of LTM, whereas stress during the late phase (12 h) after retrieval, referred to as late reconsolidation, selectively disrupted the restabilized persistence of LTM. Moreover, the effects of stress on the persistence of LTM were blocked by the corticosterone synthesis inhibitor metyrapone, which was administered before stress, suggesting that the glucocorticoid system is involved in the effects of stress on the persistence of LTM. We conclude that stress within a restricted time window after acquisition or retrieval selectively affects the persistence of LTM and depends on the glucocorticoid system. PMID:23544051

A Search for FRB 121102-like Persistent Radio-luminous Sources—Candidates and Implications for the FRB Rate and Searches

NASA Astrophysics Data System (ADS)

Ofek, Eran O.

2017-09-01

The localization of the repeating fast radio burst (FRB), FRB 121102, suggests that it is associated with a persistent radio-luminous compact source in the FRB host galaxy. Using the FIRST radio catalog, I present a search for luminous persistent sources in nearby galaxies, with radio luminosities > 10 % of the FRB 121102 persistent source luminosity. The galaxy sample contains about 30% of the total galaxy g-band luminosity within < 108 Mpc, in a footprint of 10,600 deg2. After rejecting sources likely due to active galactic nuclei activity or background sources, I am left with 11 candidates that are presumably associated with galactic disks or star-formation regions. At least some of these candidates are likely to be due to chance alignment. In addition, I find 85 sources within 1\\prime\\prime of galactic nuclei. Assuming that the radio persistent sources are not related to galactic nuclei and that they follow the galaxy g-band light, the 11 sources imply a 95% confidence upper limit on the space density of luminous persistent sources of ≲ 5× {10}-5 Mpc-3, and that at any given time only a small fraction of galaxies host a radio-luminous persistent source (≲ {10}-3 {L}* -1). Assuming a persistent source lifetime of 100 years, this implies a birth rate of ≲ 5× {10}-7 yr-1 Mpc-3. Given the FRB volumetric rate, and assuming that all FRBs repeat and are associated with persistent radio sources, this sets a lower limit on the rate of FRB events per persistent source of ≳ 0.8 yr-1. I argue that these 11 candidates are good targets for FRB searches and I estimate the FRB event rate from these candidates.

Identification of an Antimicrobial Agent Effective against Methicillin-Resistant Staphylococcus aureus Persisters Using a Fluorescence-Based Screening Strategy

PubMed Central

Kim, Wooseong; Conery, Annie L.; Rajamuthiah, Rajmohan; Fuchs, Beth Burgwyn; Ausubel, Frederick M.; Mylonakis, Eleftherios

2015-01-01

Persisters are a subpopulation of normal bacterial cells that show tolerance to conventional antibiotics. Persister cells are responsible for recalcitrant chronic infections and new antibiotics effective against persisters would be a major development in the treatment of these infections. Using the reporter dye SYTOX Green that only stains cells with permeabilized membranes, we developed a fluorescence-based screening assay in a 384-well format for identifying compounds that can kill methicillin-resistant Staphylococcus aureus (MRSA) persisters. The assay proved robust and suitable for high throughput screening (Z`-factor: >0.7). In screening a library of hits from a previous screen, which identified compounds that had the ability to block killing of the nematode Caenorhabditis by MRSA, we discovered that the low molecular weight compound NH125, a bacterial histidine kinase inhibitor, kills MRSA persisters by causing cell membrane permeabilization, and that 5 μg/mL of the compound can kill all cells to the limit of detection in a 108 CFU/mL culture of MRSA persisters within 3h. Furthermore, NH125 disrupts 50% of established MRSA biofilms at 20 μg/mL and completely eradicates biofilms at 160 μg/mL. Our results suggest that the SYTOX Green screening assay is suitable for large-scale projects to identify small molecules effective against MRSA persisters and should be easily adaptable to a broad range of pathogens that form persisters. Since NH125 has strong bactericidal properties against MRSA persisters and high selectivity to bacteria, we believe NH125 is a good anti-MRSA candidate drug that should be further evaluated. PMID:26039584

Properties, formation, and dissipation of the North Pacific Eastern Subtropical Mode Water and its impact on interannual spiciness anomalies

NASA Astrophysics Data System (ADS)

Katsura, Shota

2018-03-01

The properties, formation, and dissipation of the North Pacific Eastern Subtropical Mode Water (ESTMW), their interannual variability, and impact on spiciness anomalies in the upper permanent pycnocline were investigated using Argo profiling float data in 2005-2015. The core temperature and salinity of ESTMWs were horizontally compensated to a constant density, and core potential density concentrates in a range of 24.5-25.2 kg m-3 with two distinct peaks. ESTMWs showed different spatial distribution and persistence for its core potential density. Denser ESTMWs with a potential density of 24.9-25.2 kg m-3 were formed in winter mixed layer depth maximum centered at 30°N, 140°W and lighter ESTMWs of 24.5-24.9 kg m-3 were formed south and east of it. After formation through shoaling of the winter mixed layer, the former persisted until the following autumn and a small part of it subducted in winter, while the latter dissipated in summer. The formation region of ESTMW corresponded to the summer sea surface density maximum resulting from its poleward sea surface salinity front. Sea surface density maximum maintains weak stratification during summer, preconditioning the deepening of the winter mixed layer and hence the formation of ESTMWs. A relationship between the ESTMW formation region and the summer sea surface density maximum was also found in the North Atlantic and the South Pacific, implying the importance of sea surface salinity fronts and the associated summer sea surface density maximum to ESTMW formation. Interannual variations of ESTMW reflected that of the winter mixed layer in its formation region, and the thickness of ESTMW was related to the Pacific decadal oscillation. ESTMW contributed to the occurrence of spice injection and affected spiciness anomalies in the upper permanent pycnocline through its formation and dissipation.

Steering cell migration by alternating blebs and actin-rich protrusions.

PubMed

Diz-Muñoz, Alba; Romanczuk, Pawel; Yu, Weimiao; Bergert, Martin; Ivanovitch, Kenzo; Salbreux, Guillaume; Heisenberg, Carl-Philipp; Paluch, Ewa K

2016-09-02

High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood. Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision. Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times.

Key-value store with internal key-value storage interface

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bent, John M.; Faibish, Sorin; Ting, Dennis P. J.

A key-value store is provided having one or more key-value storage interfaces. A key-value store on at least one compute node comprises a memory for storing a plurality of key-value pairs; and an abstract storage interface comprising a software interface module that communicates with at least one persistent storage device providing a key-value interface for persistent storage of one or more of the plurality of key-value pairs, wherein the software interface module provides the one or more key-value pairs to the at least one persistent storage device in a key-value format. The abstract storage interface optionally processes one or moremore » batch operations on the plurality of key-value pairs. A distributed embodiment for a partitioned key-value store is also provided.« less

Can inertia-gravity waves persistently alter the tropopause inversion layer?

NASA Astrophysics Data System (ADS)

Kunkel, Daniel; Hoor, Peter; Wirth, Volkmar

2014-11-01

Previous simulations of baroclinic life cycles have shown, among many other features, the evolution of a tropopause inversion layer (TIL) as well as the spontaneous emission of inertia-gravity waves (IGWs). This study suggests that the latter two are related to each other, i.e., that IGWs may affect the TIL in a persistent manner. The IGWs are emitted along the jet and grow to large amplitudes, leading to the appearance of low-gradient Richardson numbers that indicate Kelvin-Helmholtz instability. Ensuing energy dissipation, local heating, and turbulence may persistently alter the thermodynamical structure of the tropopause region and, therefore, contribute to TIL formation or alter an existing TIL. Moreover, the flow in the region of the IGW favors the occurrence of wave capture, which may enhance the effect of wave breaking.

Characterization of Acinetobacter baumannii biofilm associated components

NASA Astrophysics Data System (ADS)

Brossard, Kari A.

Acinetobacter baumannii is a Gram-negative aerobic coccobaccillus that is a major cause of nosocomial infections worldwide. Infected individuals may develop pneumonia, urinary tract, wound, and other infections that are associated with the use of indwelling medical devices such as catheters and mechanical ventilation. Treatment is difficult because many A. baumannii isolates have developed multi-drug resistance and the bacterium can persist on abiotic surfaces. Persistence and resistance may be due to formation of biofilms, which leads to long-term colonization, evasion of the host immune system and resistance to treatment with antibiotics and disinfectants. While biofilms are complex multifaceted structures, two bacterial components that have been shown to be important in formation and stability are exopolysaccharides (EPS) and the biofilm-associated protein (Bap). An EPS, poly-beta-1,6-N-acetylglucosamine, PNAG, has been described for E. coli and S. epidermidis. PNAG acts as an intercellular adhesin. Production of this adhesin is dependent on the pga/icaABCD locus. We have identified a homologous locus in A. baumannii 307-0294 that is involved in production of an exopolysaccharide, recognized by an anti-PNAG antibody. We hypothesized that the A. baumannii pgaABCD locus plays a role in biofilm formation, and protection against host innate defenses and disinfectants suggesting that PNAG is a possible virulence factor for the organism. The first aim of this thesis will define the pgaABCD locus. We have previously identified Bap, a protein with similarity to those described for S. aureus and we have demonstrated that this protein is involved in maintaining the stability of biofilms on glass. We hypothesized that A. baumannii Bap plays a role in persistence and pathogenesis and is regulated by quorum sensing. In our second aim we will examine the role of Bap in attachment and biofilm formation on medically relevant surfaces and also determine if Bap is involved in epithelial cell surface attachment and invasion. Finally we will determine the effects of quorum sensing on the expression of Bap.

Impaired clearance of neutrophils extracellular trap (NET) may induce detrimental tissular effect.

PubMed

Anjos, Paula M F; Fagundes-Netto, Fernanda S; Volpe, Caroline M O; Nogueira-Machado, Jose A

2014-01-01

Neutrophils Extracellular Trap (NET) is composed of nuclear chromatin with hyper segmentation of nuclear lobes, citrullination of histone-associated DNA and mixing with cytoplasmic proteins including the enzyme myeloperoxidase. It is believed that neutrophils trap can kill microorganisms and constitutes a new form of innate defense. However, in some conditions, NET formation may be detrimental to the organism due to its association with autoantibody formation. Thus, NETs can be beneficial or detrimental depending of the DNA clearance recent registered patents describing the processes, products, methods and therapeutic indications of the neutrophil extracellular trap (NET) phenomenon have been reported. The patents US8710039; EP2465536; EP2651440; US20130302345; US20140099648; US20130183662; WO2012166611; and RU2463349C2, related to NETosis, suggest an association between NET formation and autoimmunity. However, its function is still not fully understood. Some parasites have learned to escape from NET using nucleases. NET persistence could be due to a possible enzymatic inhibition as suggested in Grabar´s theory for explaining the induction of physiologic or pathologic autoantibodies. In the present mini-review NET persistence due to impairment in the homeostasis clearance of DNA is discussed.

Perfluorocarboxylic acid (PFCA) atmospheric formation and transport to the Arctic.

NASA Astrophysics Data System (ADS)

Pike-thackray, C.; Selin, N. E.

2015-12-01

Perfluorocarboxylic acids (PFCAs) are highly persistent and toxic environmental contaminants that have been found in remote locations such as the Arctic, far from emission sources. These persistent organic pollutants are emitted directly to the atmosphere as well as being produced by the degradation of precursor compounds in the atmosphere, but recent trends towards increasing precursor emissions and decreasing direct emissions raise the importance of production in the atmosphere. Our work aims to improve understanding of the atmospheric degradation of fluorotelomer precursor compounds to form the long-chain PFCAs PFOA (C8) and PFNA (C9).Using the atmospheric chemical transport model GEOS-Chem, which uses assimilated meteorology to simulate the atmospheric transport of trace gas species, we investigate the interaction of the atmospheric formation of PFCAs and the atmospheric transport of their precursor species. Our simulations are a first application of the GEOS-Chem framework to PFCA chemistry. We highlight the importance of the spatial and temporal variability of background atmospheric chemical conditions experienced during transport. We find that yields and formation times of PFOA and PFNA respond differently and strongly to the photochemical conditions of the atmosphere, such as the abundance of NO, HO2, and other photochemical species.

Collapsed tetragonal phase as a strongly covalent and fully nonmagnetic state: Persistent magnetism with interlayer As-As bond formation in Rh-doped Ca0 .8Sr0 .2Fe2As2

NASA Astrophysics Data System (ADS)

Zhao, K.; Glasbrenner, J. K.; Gretarsson, H.; Schmitz, D.; Bednarcik, J.; Etter, M.; Sun, J. P.; Manna, R. S.; Al-Zein, A.; Lafuerza, S.; Scherer, W.; Cheng, J. G.; Gegenwart, P.

2018-02-01

A well-known feature of the CaFe2As2 -based superconductors is the pressure-induced collapsed tetragonal phase that is commonly ascribed to the formation of an interlayer As-As bond. Using detailed x-ray scattering and spectroscopy, we find that Rh-doped Ca0.8Sr0.2Fe2As2 does not undergo a first-order phase transition and that local Fe moments persist despite the formation of interlayer As-As bonds. Our density functional theory calculations reveal that the Fe-As bond geometry is critical for stabilizing magnetism and the pressure-induced drop in the c lattice parameter observed in pure CaFe2As2 is mostly due to a constriction within the FeAs planes. The collapsed tetragonal phase emerges when covalent bonding of strongly hybridized Fe 3 d and As 4 p states completely wins out over their exchange splitting. Thus the collapsed tetragonal phase is properly understood as a strong covalent phase that is fully nonmagnetic with the As-As bond forming as a by-product.

The Primordial Entropy of Jupiter

NASA Astrophysics Data System (ADS)

Cumming, Andrew; Helled, Ravit; Venturini, Julia

2018-04-01

The formation history of giant planets determines their primordial structure and consequent evolution. We simulate various formation paths of Jupiter to determine its primordial entropy, and find that a common outcome is for proto-Jupiter to have non-convective regions in its interior. We use planet formation models to calculate how the entropy and post-formation luminosity depend on model properties such as the solid accretion rate and opacity, and show that the gas accretion rate and its time evolution play a key role in determining the entropy profile. The predicted luminosity of Jupiter shortly after formation varies by a factor of 2-3 for different choices of model parameters. We find that entropy gradients inside Jupiter persist for ˜10 Myr after formation. We suggest that these gradients should be considered together with heavy-element composition gradients when modeling Jupiter's evolution and internal structure.

The primordial entropy of Jupiter

NASA Astrophysics Data System (ADS)

Cumming, Andrew; Helled, Ravit; Venturini, Julia

2018-07-01

The formation history of giant planets determines their primordial structure and consequent evolution. We simulate various formation paths of Jupiter to determine its primordial entropy, and find that a common outcome is for proto-Jupiter to have non-convective regions in its interior. We use planet formation models to calculate how the entropy and post-formation luminosity depend on model properties such as the solid accretion rate and opacity, and show that the gas accretion rate and its time evolution play a key role in determining the entropy profile. The predicted luminosity of Jupiter shortly after formation varies by a factor of 2-3 for different choices of model parameters. We find that entropy gradients inside Jupiter persist for ˜10 Myr after formation. We suggest that these gradients should be considered together with heavy-element composition gradients when modelling Jupiter's evolution and internal structure.

Review of osteoimmunology and the host response in endodontic and periodontal lesions

PubMed Central

Graves, Dana T.; Oates, Thomas; Garlet, Gustavo P.

2011-01-01

Both lesions of endodontic origin and periodontal diseases involve the host response to bacteria and the formation of osteolytic lesions. Important for both is the upregulation of inflammatory cytokines that initiate and sustain the inflammatory response. Also important are chemokines that induce recruitment of leukocyte subsets and bone-resorptive factors that are largely produced by recruited inflammatory cells. However, there are differences also. Lesions of endodontic origin pose a particular challenge since that bacteria persist in a protected reservoir that is not readily accessible to the immune defenses. Thus, experiments in which the host response is inhibited in endodontic lesions tend to aggravate the formation of osteolytic lesions. In contrast, bacteria that invade the periodontium appear to be less problematic so that blocking arms of the host response tend to reduce the disease process. Interestingly, both lesions of endodontic origin and periodontitis exhibit inflammation that appears to inhibit bone formation. In periodontitis, the spatial location of the inflammation is likely to be important so that a host response that is restricted to a subepithelial space is associated with gingivitis, while a host response closer to bone is linked to bone resorption and periodontitis. However, the persistence of inflammation is also thought to be important in periodontitis since inflammation present during coupled bone formation may limit the capacity to repair the resorbed bone. PMID:21547019

Formation and Stabilization of Environmentally Persistent Free Radicals Induced by the Interaction of Anthracene with Fe(III)-Modified Clays.

PubMed

Jia, Hanzhong; Nulaji, Gulimire; Gao, Hongwei; Wang, Fu; Zhu, Yunqing; Wang, Chuanyi

2016-06-21

Environmentally persistent free radicals (EPFRs) are occasionally detected in Superfund sites but the formation of EPFRs induced by polycyclic aromatic hydrocarbons (PAHs) is not well understood. In the present work, the formation of EPFRs on anthracene-contaminated clay minerals was quantitatively monitored via electron paramagnetic resonance (EPR) spectroscopy, and surface/interface-related environmental influential factors were systematically explored. The obtained results suggest that EPFRs are more readily formed on anthracene-contaminated Fe(III)-montmorillonite than in other tested systems. Depending on the reaction condition, more than one type of organic radicals including anthracene-based radical cations with g-factors of 2.0028-2.0030 and oxygenic carbon-centered radicals featured by g-factors of 2.0032-2.0038 were identified. The formed EPFRs are stabilized by their interaction with interlayer surfaces, and such surface-bound EPFRs exhibit slow decay with 1/e-lifetime of 38.46 days. Transformation pathway and possible mechanism are proposed on the basis of experimental results and quantum mechanical simulations. Overall, the formation of EPFRs involves single-electron-transfer from anthracene to Fe(III) initially, followed by H2O addition on formed aromatic radical cation. Because of their potential exposure in soil and atmosphere, such clay surface-associated EPFRs might induce more serious toxicity than PAHs and exerts significant impacts on human health.

Persistent axial neck pain after cervical disc arthroplasty: a radiographic analysis.

PubMed

Wagner, Scott C; Formby, Peter M; Kang, Daniel G; Van Blarcum, Gregory S; Cody, John P; Tracey, Robert W; Lehman, Ronald A

2016-07-01

There is very little literature examining optimal radiographic parameters for placement of cervical disc arthroplasty (CDA), nor is there substantial evidence evaluating the relationship between persistent postoperative neck pain and radiographic outcomes. We set out to perform a single-center evaluation of the radiographic outcomes, including associated complications, of CDA. This is a retrospective review. Two hundred eighty-five consecutive patients undergoing CDA were included in the review. The outcome measures were radiological parameters (preoperative facet arthrosis, disc height, CDA placement in sagittal and coronal planes, heterotopic ossification [HO] formation, etc.) and patient outcomes (persistent pain, recurrent pain, new-onset pain, etc.). We performed a retrospective review of all patients from a single military tertiary medical center from August 2008 to August 2012 undergoing CDA. Preoperative, immediate postoperative, and final follow-up films were evaluated. The clinical outcomes and complications associated with the procedure were also examined. The average radiographic follow-up was 13.5 months and the rate of persistent axial neck pain was 17.2%. For patients with persistent neck pain, the rate of HO formation per level studied was 22.6%, whereas the rate was significantly lower for patients without neck pain (11.7%, p=.03). There was no significant association between the severity of HO and the presence of neck pain. Patients with a preoperative diagnosis of cervicalgia, compared to those without cervicalgia, were significantly more likely to experience continued neck pain postoperatively (28.6% vs. 13.1%, p=.01). There were no differences in preoperative facet arthrosis, pre- or postoperative disc height, segmental range of motion, or placement of the device relative to the posterior edge of the vertebral body.However, patients with implants more centered between the uncovertebral joints were more likely to experience posterior neck pain (p=.03). We found that posterior axial neck pain is relatively frequent after CDA, and patients with persistent neck pain were significantly more likely to have preoperative cervicalgia and develop HO postoperatively. We also found that patients with implants that were placed off-centered were less likely to also complain of neck pain, although the reasons for this finding remain unclear. Published by Elsevier Inc.

Eradication of Pseudomonas aeruginosa cells by cathodic electrochemical currents delivered with graphite electrodes.

PubMed

Niepa, Tagbo H R; Wang, Hao; Gilbert, Jeremy L; Ren, Dacheng

2017-03-01

Antibiotic resistance is a major challenge to the treatment of bacterial infections associated with medical devices and biomaterials. One important intrinsic mechanism of such resistance is the formation of persister cells that are phenotypic variants of microorganisms and highly tolerant to antibiotics. Recently, we reported a new approach to eradicating persister cells of Pseudomonas aeruginosa using low-level direct electrochemical current (DC) and synergy with the antibiotic tobramycin. To further understand the underlying mechanism and develop this technology toward possible medical applications, we investigated the electricidal activities of non-metallic biomaterial on persister and biofilm cells of P. aeruginosa using graphite-based TGON™ 805 electrodes. We employed both single and dual chamber systems to compare electrochemical factors of TGON and stainless steel 304 electrodes. The results revealed that TGON-based treatments were highly effective against P. aeruginosa persister cells. In the single chamber system, complete eradication of planktonic persister cells (corresponding to a 7-log killing) was achieved with 70μA/cm 2 DC using TGON electrodes within 40min of treatment, while the cell viability in biofilms was reduced by 2 logs within 1h. The killing effects were dose and time dependent with higher current densities requiring less time. Moreover, reduction reactions were found more effective than oxidation reactions, confirming that metal cations are not indispensable, although they may facilitate cell killing. The findings of this study can help develop electrochemical technologies to eradicate persister and biofilm cells for more effective treatment of medical device and biomaterial associated infections. Infections associated with medical devices and biomaterials present a major challenge due to high-level tolerance of microbes to conventional antibiotics. It is well established that such tolerance is due to the formation of dormant persister cells and multicellular structures known as biofilms. Recent studies have demonstrated electrochemical treatment as a promising alternative to eradicate bacterial infections, since the killing mechanism is independent of the growth phase of bacterial cells, but relies on various electrochemical species interplaying during the treatment. The current study investigated major bactericidal properties of the electrochemical currents mediated via TGON, a carbon-based electrode material. Up to total eradication of Pseudomonas aeruginosa persister cells was achieved. The new knowledge of electrochemical properties and the bioactivity of TGON may help develop new methods/devices to eradicate bacterial infections by delivering safe levels of electrochemical currents. Copyright © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Invited review: effect, persistence, and virulence of coagulase-negative Staphylococcus species associated with ruminant udder health.

PubMed

Vanderhaeghen, W; Piepers, S; Leroy, F; Van Coillie, E; Haesebrouck, F; De Vliegher, S

2014-09-01

The aim of this review is to assess the effect of coagulase-negative staphylococci (CNS) species on udder health and milk yield in ruminants, and to evaluate the capacity of CNS to cause persistent intramammary infections (IMI). Furthermore, the literature on factors suspected of playing a role in the pathogenicity of IMI-associated CNS, such as biofilm formation and the presence of various putative virulence genes, is discussed. The focus is on the 5 CNS species that have been most frequently identified as causing bovine IMI using reliable molecular identification methods (Staphylococcus chromogenes, Staphylococcus simulans, Staphylococcus haemolyticus, Staphylococcus xylosus, and Staphylococcus epidermidis). Although the effect on somatic cell count and milk production is accepted to be generally limited or nonexistent for CNS as a group, indications are that the typical effects differ between CNS species and perhaps even strains. It has also become clear that many CNS species can cause persistent IMI, contrary to what has long been believed. However, this trait appears to be quite complicated, being partly strain dependent and partly dependent on the host's immunity. Consistent definitions of persistence and more uniform methods for testing this phenomenon will benefit future research. The factors explaining the anticipated differences in pathogenic behavior appear to be more difficult to evaluate. Biofilm formation and the presence of various staphylococcal virulence factors do not seem to (directly) influence the effect of CNS on IMI but the available information is indirect or insufficient to draw consistent conclusions. Future studies on the effect, persistence, and virulence of the different CNS species associated with IMI would benefit from using larger and perhaps even shared strain collections and from adjusting study designs to a common framework, as the large variation currently existing therein is a major problem. Also within-species variation should be investigated. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Inhibitors of biofilm formation by biofuel fermentation contaminants

USDA-ARS?s Scientific Manuscript database

Biofuel fermentation contaminants such as Lactobacillus sp. may persist in production facilities by forming recalcitrant biofilms. In this study, biofilm-forming strains of Lactobacillus brevis, L. fermentum, and L. plantarum were isolated and characterized from a dry-grind fuel ethanol plant. A var...

Biofilm formation and ethanol inhibition by bacterial contaminants of biofuel fermentation

USDA-ARS?s Scientific Manuscript database

Bacterial contaminants can inhibit ethanol production in biofuel fermentations, and even result in stuck fermentations. Contaminants may persist in production facilities by forming recalcitrant biofilms. A two-year longitudinal study was conducted of bacterial contaminants from a Midwestern dry grin...

Involvement of the host DNA-repair enzyme TDP2 in formation of the covalently closed circular DNA persistence reservoir of hepatitis B viruses.

PubMed

Königer, Christian; Wingert, Ida; Marsmann, Moritz; Rösler, Christine; Beck, Jürgen; Nassal, Michael

2014-10-07

Hepatitis B virus (HBV), the causative agent of chronic hepatitis B and prototypic hepadnavirus, is a small DNA virus that replicates by protein-primed reverse transcription. The product is a 3-kb relaxed circular DNA (RC-DNA) in which one strand is linked to the viral polymerase (P protein) through a tyrosyl-DNA phosphodiester bond. Upon infection, the incoming RC-DNA is converted into covalently closed circular (ccc) DNA, which serves as a viral persistence reservoir that is refractory to current anti-HBV treatments. The mechanism of cccDNA formation is unknown, but the release of P protein is one mandatory step. Structural similarities between RC-DNA and cellular topoisomerase-DNA adducts and their known repair by tyrosyl-DNA-phosphodiesterase (TDP) 1 or TDP2 suggested that HBV may usurp these enzymes for its own purpose. Here we demonstrate that human and chicken TDP2, but only the yeast ortholog of TDP1, can specifically cleave the Tyr-DNA bond in virus-adapted model substrates and release P protein from authentic HBV and duck HBV (DHBV) RC-DNA in vitro, without prior proteolysis of the large P proteins. Consistent with TPD2's having a physiological role in cccDNA formation, RNAi-mediated TDP2 depletion in human cells significantly slowed the conversion of RC-DNA to cccDNA. Ectopic TDP2 expression in the same cells restored faster conversion kinetics. These data strongly suggest that TDP2 is a first, although likely not the only, host DNA-repair factor involved in HBV cccDNA biogenesis. In addition to establishing a functional link between hepadnaviruses and DNA repair, our results open new prospects for directly targeting HBV persistence.

Selective Denervation for Persistent Knee Pain After Total Knee Arthroplasty: A Report of 50 Cases.

PubMed

Shi, Shao-Min; Meister, David W; Graner, Kelly C; Ninomiya, James T

2017-03-01

Despite the general success of total knee arthroplasty (TKA), up to 20% of patients report dissatisfaction following surgery. One potential cause of this dissatisfaction is residual pain secondary to neuroma formation in the sensory nerve branches that innervate the knee. We found, after performing a retrospective review, that up to 9.7% of patients following primary TKA and up to 21% of revision cases exhibited persistent knee pain attributable to neuroma formation. Despite the high incidence of this pathology, little is known about the effective diagnosis or treatment of neuroma formation following TKA. Between 2011 and 2014, 50 patients with persistent symptomatic neuroma pain following TKA underwent selective denervation. These patients had demonstrated the appropriate selection criteria and had failed conservative management. Patients were evaluated by the visual analog scale pain score and the Knee Society Score to determine the outcome of the described treatment. Thirty-two patients (64%) rated their outcome as excellent, 10 (20%) as good, 3 (6%) as fair, and 2 (4%) reported no change. The mean visual analog scale pain score was improved from 9.4 ± 0.8 to 1.1 ± 1.6 following surgery (P ≤ .001). The mean Knee Society Scores increased from 45.5 ± 14.3 to 94.1 ± 8.6 points (P ≤ .0001). Three patients (6%) required the second neurectomy due to recurrent pain and received excellent pain relief postoperatively. There were 2 complications of superficial skin peri-incisional hyperemia related to dressings. Average follow-up duration was 24 months (range, 16-38 months). Our study suggests that selective denervation provides an effective and long-lasting option for the management of this pathology. Copyright © 2016 Elsevier Inc. All rights reserved.

Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks

PubMed Central

Yang, Hee-Jeong; Bogomolnaya, Lydia M.; Elfenbein, Johanna R.; Endicott-Yazdani, Tiana; Reynolds, M. Megan; Porwollik, Steffen; Cheng, Pui; Xia, Xiao-Qin

2016-01-01

Contaminated chicken/egg products are major sources of human salmonellosis, yet the strategies used by Salmonella to colonize chickens are poorly understood. We applied a novel two-step hierarchical procedure to identify new genes important for colonization and persistence of Salmonella enterica serotype Typhimurium in chickens. A library of 182 S. Typhimurium mutants each containing a targeted deletion of a group of contiguous genes (for a total of 2,069 genes deleted) was used to identify regions under selection at 1, 3, and 9 days postinfection in chicks. Mutants in 11 regions were under selection at all assayed times (colonization mutants), and mutants in 15 regions were under selection only at day 9 (persistence mutants). We assembled a pool of 92 mutants, each deleted for a single gene, representing nearly all genes in nine regions under selection. Twelve single gene deletion mutants were under selection in this assay, and we confirmed 6 of 9 of these candidate mutants via competitive infections and complementation analysis in chicks. STM0580, STM1295, STM1297, STM3612, STM3615, and STM3734 are needed for Salmonella to colonize and persist in chicks and were not previously associated with this ability. One of these key genes, STM1297 (selD), is required for anaerobic growth and supports the ability to utilize formate under these conditions, suggesting that metabolism of formate is important during infection. We report a hierarchical screening strategy to interrogate large portions of the genome during infection of animals using pools of mutants of low complexity. Using this strategy, we identified six genes not previously known to be needed during infection in chicks, and one of these (STM1297) suggests an important role for formate metabolism during infection. PMID:26857572

The topology of the cosmic web in terms of persistent Betti numbers

NASA Astrophysics Data System (ADS)

Pranav, Pratyush; Edelsbrunner, Herbert; van de Weygaert, Rien; Vegter, Gert; Kerber, Michael; Jones, Bernard J. T.; Wintraecken, Mathijs

2017-03-01

We introduce a multiscale topological description of the Megaparsec web-like cosmic matter distribution. Betti numbers and topological persistence offer a powerful means of describing the rich connectivity structure of the cosmic web and of its multiscale arrangement of matter and galaxies. Emanating from algebraic topology and Morse theory, Betti numbers and persistence diagrams represent an extension and deepening of the cosmologically familiar topological genus measure and the related geometric Minkowski functionals. In addition to a description of the mathematical background, this study presents the computational procedure for computing Betti numbers and persistence diagrams for density field filtrations. The field may be computed starting from a discrete spatial distribution of galaxies or simulation particles. The main emphasis of this study concerns an extensive and systematic exploration of the imprint of different web-like morphologies and different levels of multiscale clustering in the corresponding computed Betti numbers and persistence diagrams. To this end, we use Voronoi clustering models as templates for a rich variety of web-like configurations and the fractal-like Soneira-Peebles models exemplify a range of multiscale configurations. We have identified the clear imprint of cluster nodes, filaments, walls, and voids in persistence diagrams, along with that of the nested hierarchy of structures in multiscale point distributions. We conclude by outlining the potential of persistent topology for understanding the connectivity structure of the cosmic web, in large simulations of cosmic structure formation and in the challenging context of the observed galaxy distribution in large galaxy surveys.

Past Peatland Distribution as an Indicator of Hydroclimate and Temperature

NASA Astrophysics Data System (ADS)

Treat, C. C.; Jones, M.; Lacourse, T.; Payne, R.; Peteet, D. M.; Sannel, B.; Stelling, J.; Talbot, J.; Williams, C. J.; Kleinen, T.; Grosse, G.; Yu, Z.; Finkelstein, S. A.; Broothaerts, N.; Dommain, R.; Kuhry, P.; Lähteenoja, O.; Dalton, A.; Notebaert, B.; Swindles, G. T.; Tarnocai, C.; Verstraeten, G.; Xia, Z.; Brovkin, V.

2016-12-01

Peatlands, wetlands with > 30 cm of organic sediment, cover more than 3 x 106 km2 of the earth surface and have been accumulating carbon and sediments throughout the Holocene. The location of peatland formation and accumulation has been dynamic over time, as peat formation in areas like Alaska and the West Siberian Lowlands preceded peat formation in Fennoscandia and Eastern North America due to more favorable climate for peat formation. Using the geographic distribution of peatlands in the past can indicate general climatic conditions, including hydroclimate, given that the underlying geology is well understood. Peatlands form under a variety of climatic conditions and landscape positions but do not persist under arid conditions, instead requiring either humid conditions or cold temperatures. However, peatlands may have existed in the past in areas not currently suitable for peatland formation and persistence, but where peats can be found at depth within the sediment column. Here we map the locations of histic paleosols, relict peat, and buried peats since the Last Glacial Maximum using a compilation of sites from previous studies. We compare these records of past peatland distribution to present-day peatland distribution. We evaluate regional differences in timing of peatland development in these buried peatlands to the development of extant peatlands. Finally, we compare the timing of past peatland extent to the to modeled paleoclimate during the Quaternary. In addition to implications for paleoclimate, these past peatlands are not well accounted for in present-day soil carbon stocks but could be an important component of deep soil carbon pools.

Observation of rocket pollution with overhead sensors

NASA Astrophysics Data System (ADS)

Fisher, Annette

2011-12-01

The objective of this thesis is to study the dispersal of rocket pollution through remote sensing techniques. Substantial research with remote sensors has been dedicated to observation of volcanic plumes, particulate dispersion, and aircraft contrails with less emphasis on observing rocket launches and the effects on the surrounding environment. This research focuses on observation of rocket exhaust constituents, particularly carbon soot, alumina, and water vapor. The sensors utilized in this thesis have unique capabilities that provide measurements that are likely capable of detecting the rocket exhaust constituents. Methodology and analysis included choosing an appropriate launch vehicle with obtainable launch data and various booster combinations of liquid propellant only or a combination of liquid and solid propellant, prioritizing the data based on launch time versus sensor passing, processing the data, and applying known constituent properties to the data sets where key areas of work in this endeavor. Results of this work demonstrate a unique capability in monitoring man-made pollution and the extent the pollution can spread to surrounding areas.

Bromoxynil Degradation in a Mississippi Silt Loam Soil

USDA-ARS?s Scientific Manuscript database

There is a paucity of data on bromoxynil fate in agricultural soils. The objective of these laboratory experiments were to 1) assess bromoxynil sorption, mineralization, bound residue formation, and persistence of extractable residues in native (i.e., non-autoclaved) Dundee silt loam soil collecte...

Formation and stability of impurity "snakes" in tokamak plasmas.

PubMed

Delgado-Aparicio, L; Sugiyama, L; Granetz, R; Gates, D A; Rice, J E; Reinke, M L; Bitter, M; Fredrickson, E; Gao, C; Greenwald, M; Hill, K; Hubbard, A; Hughes, J W; Marmar, E; Pablant, N; Podpaly, Y; Scott, S; Wilson, R; Wolfe, S; Wukitch, S

2013-02-08

New observations of the formation and dynamics of long-lived impurity-induced helical "snake" modes in tokamak plasmas have recently been carried out on Alcator C-Mod. The snakes form as an asymmetry in the impurity ion density that undergoes a seamless transition from a small helically displaced density to a large crescent-shaped helical structure inside q<1, with a regularly sawtoothing core. The observations show that the conditions for the formation and persistence of a snake cannot be explained by plasma pressure alone. Instead, many features arise naturally from nonlinear interactions in a 3D MHD model that separately evolves the plasma density and temperature.

Commonly prescribed β-lactam antibiotics induce C. trachomatis persistence/stress in culture at physiologically relevant concentrations

PubMed Central

Kintner, Jennifer; Lajoie, Dawn; Hall, Jennifer; Whittimore, Judy; Schoborg, Robert V.

2014-01-01

Chlamydia trachomatis, the most common bacterial sexually transmitted disease agent worldwide, enters a viable, non-dividing and non-infectious state (historically termed persistence and more recently referred to as the chlamydial stress response) when exposed to penicillin G in culture. Notably, penicillin G-exposed chlamydiae can reenter the normal developmental cycle upon drug removal and are resistant to azithromycin-mediated killing. Because penicillin G is less frequently prescribed than other β-lactams, the clinical relevance of penicillin G-induced chlamydial persistence/stress has been questioned. The goal of this study was to determine whether more commonly used penicillins also induce C. trachomatis serovar E persistence/stress. All penicillins tested, as well as clavulanic acid, induced formation of aberrant, enlarged reticulate bodies (RB) (called aberrant bodies or AB) characteristic of persistent/stressed chlamydiae. Exposure to the penicillins and clavulanic acid also reduced chlamydial infectivity by >95%. None of the drugs tested significantly reduced chlamydial unprocessed 16S rRNA or genomic DNA accumulation, indicating that the organisms were viable, though non-infectious. Finally, recovery assays demonstrated that chlamydiae rendered essentially non-infectious by exposure to ampicillin, amoxicillin, carbenicillin, piperacillin, penicillin V, and clavulanic acid recovered infectivity after antibiotic removal. These data definitively demonstrate that several commonly used penicillins induce C. trachomatis persistence/stress at clinically relevant concentrations. PMID:24783061

Microbes Persist: Using a Systems Biology Approach to Reveal How the Soil Microbiome Shapes Soil Organic Matter

NASA Astrophysics Data System (ADS)

Pett-Ridge, J.

2017-12-01

Soils store more carbon than the atmosphere and terrestrial vegetation combined, yet the factors that control its persistence remain elusive. Recent insights have overturned the long-held assumption that carbon stability depends mostly on chemical `recalcitrance' of soil organic matter (SOM). Instead, an emerging paradigm emphasizes how environmental drivers like temperature and moisture, soil minerals, and microbial ecology interact to control SOM formation, stabilization, and turnover. Detailed spectroscopic and isotopic (14C) analyses of mineral-associated SOM show that the oldest carbon in soil may be easily broken down and respired in the laboratory, and that it biochemically resembles microbial cells and metabolites far more than plant material. This places microbial ecophysiology at the center of the soil carbon persistence question. Microbial cells likely interact with mineral surfaces as part of an ecological strategy to condition their environment (e.g. biofilm formation or extracellular enzyme production), and their diverse cellular components likely associate with minerals after cells die. Collectively, these microbial characteristics - metabolic activities, population growth strategies, and cellular biochemistry - can be thought of as `soil ecophysiological traits'. This presentation will explore potential traits that may be fruitful targets for studies evaluating the persistence and importance of microbial products as SOM precursors, and will highlight results showing that soil mineral type influences the microbial communities that colonize mineral surfaces, as well as the quantity and type of mineral-associated carbon that accumulates. I will propose a series of integrated approaches that used together can examine how genomic capacity and activities of soil microbiomes are shaped by edaphic conditions (moisture, temperature, redox regimes) and fundamentally affect the terrestrial soil C pool.

Optimizing Blue Persistent Luminescence in (Sr 1-δ Ba δ ) 2 MgSi 2 O 7: Eu 2+ ,Dy 3+ via Solid Solution for Use in Point-of-Care Diagnostics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Finley, Erin; Cobb, Angelica; Duke, Anna

Inorganic persistent luminescent phosphors are an excellent class of optical reporters for enabling sensitive point-of-care diagnostics, particularly with smartphone-based biosensing devices in testing formats such as the lateral flow assay (LFA). Here, the development of persistent phosphors for this application is focused on the solid solution (Sr 1-δBa δ) 2MgSi 2O 7:Eu 2+,Dy 3+ (δ = 0, 0.125, 0.25, 0.375), which is prepared using a high-temperature solid-state reaction as confirmed by synchrotron X-ray powder diffraction. The substitution of barium for strontium enables control over the Eu 2+ 5d-orbital crystal field splitting (CFS) as a tool for tuning the emission wavelengthmore » while maintaining luminescence lifetimes >9 min across the composition range. Thermoluminescence measurements of the solid solution provide evidence that trap states contribute to the persistent lifetimes with the trap depths also remaining constant as a function of composition. Time-gated luminescence images of these compounds are captured on a smartphone arranged in a layout to mimic a point-of-care test and demonstrate the viability of using these materials as optical reporters. Moreover, comparing the blue-emitting (Sr 0.625Ba 0.375) 2MgSi 2O 7:Eu 2+,Dy 3+ and the green-emitting SrAl 2O 4:Eu 2+,Dy 3+ in a single LFA-type format shows these two compounds can be detected and resolved simultaneously, thereby permitting the development of a multiplexed LFA.« less

A New Paradigm for New Oceans

NASA Astrophysics Data System (ADS)

Foulger, G. R.; Doré, A. G.; Franke, D.; Geoffroy, L.; Gernigon, L.; Hole, M.; Hoskuldsson, A.; Julian, B. R.; Kusznir, N.; Martinez, F.; Natland, J. H.; Peace, A.; Petersen, K. D.; Schiffer, C.; Stephenson, R.; Stoker, M. S.

2017-12-01

The original simple theory of plate tectonics had to be refined to accommodate second-order geological features such as back-arc basins and continental deformation zones. We propose an additional refinement that is required by complexities that form and persist in new oceans when inhomogeneous continental lithosphere/tectosphere disintegrates. Such complexities include continual plate-boundary reorganizations and migrations, distributed continental material in the ocean, propagating and dying ridges, and sagging, flexing and tilting in the oceans and at continent-ocean boundary zones. Reorganizations of stress and motion persist, resulting in variable orientations over short distances, tectonic reactivations, complex plate boundary configurations including multiple triple junctions, and the formation and abandonment of oceanic microplates. Resulting local compressions and extensions are manifest as bathymetric anomalies, vertical motions, and distributed volcanism at various times and places as the new ocean grows. Examples of regions that exhibit some or all of these features include the North Atlantic, the Rio Grande Rise/Walvis Ridge region of the South Atlantic, and the Seychelles-Mauritius region in the Indian Ocean. We suggest that these complexities arise as a result of the formation of new spreading plate boundaries by rifts propagating through continental lithosphere/tectosphere that is anisotropic as a result of inherited structure/composition and/or a sub-lithospheric mantle destabilized by lithospheric-controlled processes. Such scenarios result in complicated disintegration of continents and local persistent dynamic instability in the new ocean.

Vaginal thread formation in the healthy offspring of untreated Long-Evans rats

EPA Science Inventory

Vaginal threads are characterized as cords of mesenchymal tissue that cross the vaginal opening. They are sometimes apparent in rats after weaning, and typically disappear within 1-2 days as the female reaches puberty. If persistent, they can increase uncertainty in assessing rep...

A Psychoeducational Support Group for Serious Mental Illness

ERIC Educational Resources Information Center

Lefley, Harriet P.

2009-01-01

The formation, structure, and goals of an open-ended psychoeducational support group for people with serious and persistent mental illnesses are described, differentiating psychoeducation from psychotherapy, and professional from peer-led support groups. Major goals are to provide education for illness management and help members combat social…

Exploring the Impacts of Social Networking Sites on Academic Relations in the University

ERIC Educational Resources Information Center

Rambe, Patient

2011-01-01

Social networking sites (SNS) affordances for persistent interaction, collective generation of knowledge, and formation of peer-based clusters for knowledge sharing render them useful for developing constructivist knowledge environments. However, notwithstanding their academic value, these environments are not necessarily insulated from the…

Direct evaluation of Pseudomonas aeruginosa biofilm mediators in a chronic infection model.

PubMed

Byrd, Matthew S; Pang, Bing; Hong, Wenzhou; Waligora, Elizabeth A; Juneau, Richard A; Armbruster, Chelsie E; Weimer, Kristen E D; Murrah, Kyle; Mann, Ethan E; Lu, Haiping; Sprinkle, April; Parsek, Matthew R; Kock, Nancy D; Wozniak, Daniel J; Swords, W Edward

2011-08-01

Biofilms contribute to Pseudomonas aeruginosa persistence in a variety of diseases, including cystic fibrosis, burn wounds, and chronic suppurative otitis media. However, few studies have directly addressed P. aeruginosa biofilms in vivo. We used a chinchilla model of otitis media, which has previously been used to study persistent Streptococcus pneumoniae and Haemophilus influenzae infections, to show that structures formed in vivo are biofilms of bacterial and host origin within a matrix that includes Psl, a P. aeruginosa biofilm polysaccharide. We evaluated three biofilm and/or virulence mediators of P. aeruginosa known to affect biofilm formation in vitro and pathogenesis in vivo--bis-(3',5')-cyclic dimeric GMP (c-di-GMP), flagella, and quorum sensing--in a chinchilla model. We show that c-di-GMP overproduction has a positive impact on bacterial persistence, while quorum sensing increases virulence. We found no difference in persistence attributed to flagella. We conclude from these studies that a chinchilla otitis media model provides a means to evaluate pathogenic mediators of P. aeruginosa and that in vitro phenotypes should be examined in multiple infection systems to fully understand their role in disease.

The travel-related carbon dioxide emissions of atmospheric researchers

NASA Astrophysics Data System (ADS)

Stohl, A.

2008-11-01

Most atmospheric scientists agree that greenhouse gas emissions have already caused significant changes to the global climate system and that these changes will accelerate in the near future. At the same time, atmospheric scientists who like other scientists rely on international collaboration and information exchange travel a lot and, thereby, cause substantial emissions of CO2. In this paper, the CO2 emissions of the employees working at an atmospheric research institute (the Norwegian Institute for Air Research, NILU) caused by all types of business travel (conference visits, workshops, field campaigns, instrument maintainance, etc.) were calculated for the years 2005 2007. It is estimated that more than 90% of the emissions were caused by air travel, 3% by ground travel and 5% by hotel usage. The travel-related annual emissions were between 1.9 and 2.4 t CO2 per employee or between 3.9 and 5.5 t CO2 per scientist. For comparison, the total annual per capita CO2 emissions are 4.5 t worldwide, 1.2 t for India, 3.8 t for China, 5.9 t for Sweden and 19.1 t for Norway. The travel-related CO2 emissions of a NILU scientist, occurring in 24 days of a year on average, exceed the global average annual per capita emission. Norway's per-capita CO2 emissions are among the highest in the world, mostly because of the emissions from the oil industry. If the emissions per NILU scientist derived in this paper are taken as representative for the average Norwegian researcher, travel by Norwegian scientists would nevertheless account for a substantial 0.2% of Norway's total CO2 emissions. Since most of the travel-related emissions are due to air travel, water vapor emissions, ozone production and contrail formation further increase the relative importance of NILU's travel in terms of radiative forcing.

The travel-related carbon dioxide emissions of atmospheric researchers

NASA Astrophysics Data System (ADS)

Stohl, A.

2008-04-01

Most atmospheric scientists agree that greenhouse gas emissions have already caused significant changes to the global climate system and that these changes will accelerate in the near future. At the same time, atmospheric scientists who - like other scientists - rely on international collaboration and information exchange travel a lot and, thereby, cause substantial emissions of carbon dioxide (CO2). In this paper, the CO2 emissions of the employees working at an atmospheric research institute (the Norwegian Institute for Air Research, NILU) caused by all types of business travel (conference visits, workshops, field campaigns, instrument maintainance, etc.) were calculated for the years 2005-2007. It is estimated that more than 90% of the emissions were caused by air travel, 3% by ground travel and 5% by hotel usage. The travel-related annual emissions were between 1.9 and 2.4 t CO2 per employee or between 3.9 and 5.5 t CO2 per scientist. For comparison, the total annual per capita CO2 emissions are 4.5 t worldwide, 1.2 t for India, 3.8 t for China, 5.9 t for Sweden and 19.1 t for Norway. The travel-related CO2 emissions of a NILU scientist, occurring in 24 days of a year on average, exceed the global average annual per capita emission. Norway's per-capita CO2 emissions are among the highest in the world, mostly because of the emissions from the oil industry. If the emissions per NILU scientist derived in this paper are taken as representative for the average Norwegian researcher, travel by Norwegian scientists would nevertheless account for a substantial 0.2% of Norway's total CO2 emissions. Since most of the travel-related emissions are due to air travel, water vapor emissions, ozone production and contrail formation further increase the relative importance of NILU's travel in terms of radiative forcing.

Role Of High Speed Photography In The Testing Capabilities Of The Arnold Engineering Development Center (AEDC) Range And Track Facilities

NASA Astrophysics Data System (ADS)

Hendrix, Roy E.; Dugger, Paul H.

1983-03-01

Since the onset of user testing in the AEDC aeroballistic ranges in 1961, concentrated efforts in such areas as model launching techniques, test environment simulation, and specialized instrumentation have been made to enhance the usefulness of these test facilities. A wide selection of specialized instrumentation has been developed over the years to provide, among other features, panoramic photographic coverage of test models during flight. Pulsed ruby lasers, xenon flash lamps, visible-light spark sources, and flash X-ray systems are employed as short-duration radiation sources in various front-light and back-light photographic systems. Visible-light and near infrared image intensifier diodes are used to achieve high-speed shuttering in photographic pyrometry systems that measure surface temperatures of test models in flight. Turbine-driven framing cameras are used to provide multiframe photography of such high-speed phenomena as impact debris formation and model encounter with erosive fields. As a result, the capabilities of these ballistic range test units have increased significantly in regard to the types of tests that can be accommodated and to the quality and quantity of data that can be provided. Presently, five major range and companion track facilities are active in conducting hypervelocity testing in AEDC's von K6rman Gas Dynamics Facility (VKF): Ranges G, K, and S-1 and Tracks G and K. The following types of tests are conducted in these test units: ablation/erosion, transpiration-cooled nosetip (TCNT), nosetip transition, heat transfer, aerodynamic, cannon projectile, rocket contrail, reentry physics, and hypervelocity impact. The parallel achievements in high-speed photography and testing capabilities are discussed, and the significant role of photographic systems in the development of the overall testing capabilities of the AEDC range and track facilities is illustrated in numerous examples of photographic results.

A study of extractive and remote-sensing sampling and measurement of emissions from military aircraft engines

NASA Astrophysics Data System (ADS)

Cheng, Meng-Dawn; Corporan, Edwin

2010-12-01

Aircraft emissions contribute to the increased atmospheric burden of particulate matter (PM) that plays an important role in air quality, human health, visibility, contrail formation and climate change. Sampling and measurement of modern aircraft emissions at the engine exhaust plane (EEP) for engine and fuel certification remains challenging, as no agency-certified method is available. In this paper we summarize the results of three recent field studies devoted to investigate the consistency and applicability of "extractive" and "optical remote-sensing" (ORS) technologies in the sampling and measurement of gaseous and PM emitted by a number of military aircraft engines. Three classes of military engines were investigated; these include T56, TF33, and T700 & T701C types of engines, which consume 70-80% of the military aviation fuel each year. JP-8 and Fischer-Tropsch (FT)-derived paraffinic fuels were used to study the effect of fuels. It was found that non-volatile particles in the engine emissions were in the 20 nm range for the low power condition of new helicopter engines to 80 nm for the high power condition of legacy engines. Elemental analysis indicated little metals were present on particles, while most of the materials on the exhaust particles were carbon and sulfate based. Alkanes, carbon monoxide, carbon dioxide, nitrogen oxides, sulfur dioxide, formaldehyde, ethylene, acetylene and propylene were detected. The last five species were most noticeable only under low engine power. The emission indices calculated based on the ORS data deviate significantly from those based on the extractive data. Nevertheless, the ORS techniques were useful in the sense that it provided non-intrusive real-time detection of species in the exhaust plume, which warrants further development. The results obtained in this program help validate sampling methodology and measurement techniques used for non-volatile PM aircraft emissions as described in the SAE AIR6037 (2009).

Copper oxide-based model of persistent free radical formation on combustion-derived particulate matter.

PubMed

Lomnicki, Slawo; Truong, Hieu; Vejerano, Eric; Dellinger, Barry

2008-07-01

We have found that environmentally persistent free radicals (PFRs) are formed by adsorption of substituted aromatic molecular precursors on the surface of cupric oxide-containing particles at temperatures between 100 and 400 degrees C. This temperature range corresponds to the conditions in the postflame, cool zone of combustion, and thermal processes. Depending upon the nature of the precursor and the adsorption temperature, both substituted phenoxyl and semiquinone radicals are formed. The PFRs are formed through a mechanism of initial physisorption, followed by chemisorption via elimination of water or hydrogen chloride, and electron transfer resulting in the simultaneous reduction of Cu(II) to Cu(I) and formation of the PFR. The PFRs are still observable by electron paramagnetic resonance (EPR) after exposure to air for more than a day. Their lifetimes under vacuum appear to be infinite. Other redox-active transition metals such as iron are expected to also mediate or catalyze the formation of PFRs. The properties of the observed radicals are consistent with radicals previously observed on airborne and combustion-generated particulate matter. We propose a catalytic biochemical cycle for both the particle-associated semiquinone and phenoxyl PFRs that result in the formation of hydroxyl radical and other reactive oxygen species (ROS). This suggests that combustion-generated, particle-associated PFRs may be responsible for the oxidative stress resulting in cardiopulmonary disease and probably cancer that has been attributed to exposure to airborne fine particles.

Providing Geographic Datasets as Linked Data in Sdi

NASA Astrophysics Data System (ADS)

Hietanen, E.; Lehto, L.; Latvala, P.

2016-06-01

In this study, a prototype service to provide data from Web Feature Service (WFS) as linked data is implemented. At first, persistent and unique Uniform Resource Identifiers (URI) are created to all spatial objects in the dataset. The objects are available from those URIs in Resource Description Framework (RDF) data format. Next, a Web Ontology Language (OWL) ontology is created to describe the dataset information content using the Open Geospatial Consortium's (OGC) GeoSPARQL vocabulary. The existing data model is modified in order to take into account the linked data principles. The implemented service produces an HTTP response dynamically. The data for the response is first fetched from existing WFS. Then the Geographic Markup Language (GML) format output of the WFS is transformed on-the-fly to the RDF format. Content Negotiation is used to serve the data in different RDF serialization formats. This solution facilitates the use of a dataset in different applications without replicating the whole dataset. In addition, individual spatial objects in the dataset can be referred with URIs. Furthermore, the needed information content of the objects can be easily extracted from the RDF serializations available from those URIs. A solution for linking data objects to the dataset URI is also introduced by using the Vocabulary of Interlinked Datasets (VoID). The dataset is divided to the subsets and each subset is given its persistent and unique URI. This enables the whole dataset to be explored with a web browser and all individual objects to be indexed by search engines.

Light-Induced Peroxide Formation in ZnO: Origin of Persistent Photoconductivity

PubMed Central

Kang, Youngho; Nahm, Ho-Hyun; Han, Seungwu

2016-01-01

The persistent photoconductivity (PPC) in ZnO has been a critical problem in opto-electrical devices employing ZnO such as ultraviolet sensors and thin film transistors for the transparent display. While the metastable state of oxygen vacancy (VO) is widely accepted as the microscopic origin of PPC, recent experiments on the influence of temperature and oxygen environments are at variance with the VO model. In this study, using the density-functional theory calculations, we propose a novel mechanism of PPC that involves the hydrogen-zinc vacancy defect complex (2H-VZn). We show that a substantial amount of 2H-VZn can exist during the growth process due to its low formation energy. The light absorption of 2H-VZn leads to the metastable state that is characterized by the formation of (peroxide) around the defect, leaving the free carriers in the conduction band. Furthermore, we estimate the lifetime of photo-electrons to be ~20 secs, which is similar to the experimental observation. Our model also explains the experimental results showing that PPC is enhanced (suppressed) in oxygen-rich (low-temperature) conditions. By revealing a convincing origin of PPC in ZnO, we expect that the present work will pave the way for optimizing optoelectronic properties of ZnO. PMID:27748378

System and Method for Providing a Climate Data Persistence Service

NASA Technical Reports Server (NTRS)

Schnase, John L. (Inventor); Ripley, III, William David (Inventor); Duffy, Daniel Q. (Inventor); Thompson, John H. (Inventor); Strong, Savannah L. (Inventor); McInerney, Mark (Inventor); Sinno, Scott (Inventor); Tamkin, Glenn S. (Inventor); Nadeau, Denis (Inventor)

2018-01-01

A system, method and computer-readable storage devices for providing a climate data persistence service. A system configured to provide the service can include a climate data server that performs data and metadata storage and management functions for climate data objects, a compute-storage platform that provides the resources needed to support a climate data server, provisioning software that allows climate data server instances to be deployed as virtual climate data servers in a cloud computing environment, and a service interface, wherein persistence service capabilities are invoked by software applications running on a client device. The climate data objects can be in various formats, such as International Organization for Standards (ISO) Open Archival Information System (OAIS) Reference Model Submission Information Packages, Archive Information Packages, and Dissemination Information Packages. The climate data server can enable scalable, federated storage, management, discovery, and access, and can be tailored for particular use cases.

Antibiotic regimen based on population analysis of residing persister cells eradicates Staphylococcus epidermidis biofilms

PubMed Central

Yang, Shoufeng; Hay, Iain D.; Cameron, David R.; Speir, Mary; Cui, Bintao; Su, Feifei; Peleg, Anton Y.; Lithgow, Trevor; Deighton, Margaret A.; Qu, Yue

2015-01-01

Biofilm formation is a major pathogenicity strategy of Staphylococcus epidermidis causing various medical-device infections. Persister cells have been implicated in treatment failure of such infections. We sought to profile bacterial subpopulations residing in S. epidermidis biofilms, and to establish persister-targeting treatment strategies to eradicate biofilms. Population analysis was performed by challenging single biofilm cells with antibiotics at increasing concentrations ranging from planktonic minimum bactericidal concentrations (MBCs) to biofilm MBCs (MBCbiofilm). Two populations of “persister cells” were observed: bacteria that survived antibiotics at MBCbiofilm for 24/48 hours were referred to as dormant cells; those selected with antibiotics at 8 X MICs for 3 hours (excluding dormant cells) were defined as tolerant-but-killable (TBK) cells. Antibiotic regimens targeting dormant cells were tested in vitro for their efficacies in eradicating persister cells and intact biofilms. This study confirmed that there are at least three subpopulations within a S. epidermidis biofilm: normal cells, dormant cells, and TBK cells. Biofilms comprise more TBK cells and dormant cells than their log-planktonic counterparts. Using antibiotic regimens targeting dormant cells, i.e. effective antibiotics at MBCbiofilm for an extended period, might eradicate S. epidermidis biofilms. Potential uses for this strategy are in antibiotic lock techniques and inhaled aerosolized antibiotics. PMID:26687035

Burial increases seed longevity of two Artemisia tridentata (Asteraceae) subspecies

USGS Publications Warehouse

Wijayratne, Upekala C.; Pyke, David A.

2012-01-01

Premise of the study: Seed longevity and persistence in soil seed banks may be especially important for population persistence in ecosystems where opportunities for seedling establishment and disturbance are unpredictable. The fire regime, an important driver of population dynamics in sagebrush steppe ecosystems, has been altered by exotic annual grass invasion. Soil seed banks may play an active role in postfire recovery of the foundation shrub Artemisia tridentata, yet conditions under which seeds persist are largely unknown. Methods: We investigated seed longevity of two Artemisia tridentata subspecies in situ by retrieving seed bags that were placed at varying depths over a 2 yr period. We also sampled naturally dispersed seeds in litter and soil immediately after seed dispersal and before flowering in subsequent seasons to estimate seed persistence. Key results: After 24 mo, seeds buried at least 3 cm below the soil surface retained 30–40% viability whereas viability of seeds on the surface and under litter declined to 0 and Artemisia tridentata has the potential to form a short-term soil seed bank that persists longer than has been commonly assumed, and that burial is necessary for seed longevity. Use of seeding techniques that promote burial of some seeds to aid in formation of a soil seed bank may increase restoration potential.

Buoyancy of the ''Y2K'' Persistent Train and the Trajectory of the 04:00:29 UT Leonid Fireball

NASA Technical Reports Server (NTRS)

Jenniskens, Peter; Rairden, Rick L.; DeVincenzi, Donald L. (Technical Monitor)

2000-01-01

The atmospheric trajectory is calculated of a particularly well studied fireball and train during the 1999 Leonid Multi-Instrument Aircraft Campaign. Less than a minute after the meteor's first appearance, the train curves into a '2'-shape, which persisted until at least 13 minutes after the fireball. We conclude that the shape results because of horizontal winds from gravity waves with a scale height of 8.3 km at 79-91 km altitude, as well as a westerly wind gradient with altitude. In addition, there is downward drift that affects the formation of loops in the train early on.

Buoyancy of the "Y2K" Persistent Train and the Trajectory of the 04:00:29 UT Leonid Fireball

NASA Astrophysics Data System (ADS)

Jenniskens, Peter; Rairden, Rick L.

The atmospheric trajectory is calculated of a particularly well studied fireball and train during the 1999 Leonid Multi-Instrument Aircraft Campaign. Less than a minute after the meteor's first appearance, the train curves into a "2"-shape, which persisted until at least 13 minutes after the fireball. We conclude that the shape results because of horizontal winds from gravity waves with a scale height of 8.3 km at 79-91 km altitude, as well as a westerly wind gradient with altitude. In addition, there is downward drift that affects the formation of loops in the train early on.

ATF4, A Novel Mediator of the Anabolic Actions of PTH on Bone

DTIC Science & Technology

2009-07-01

formation rate and bone mineral density (severe osteoporosis) that persists throughout life. The expression of both osteocalcin (Ocn) and bone sialoprotein ...established that ATF4 is critical for osteoblast differentiation as demonstrated by dramatically reduced expression of osteocalcin and bone sialoprotein mRNA

The Bordetella Bps polysaccharide is required for biofilm formation and persistence in the lower respiratory tract of swine

USDA-ARS?s Scientific Manuscript database

Bordetella bronchiseptica is pervasive in swine populations and plays multiple roles in respiratory disease. Additionally, B. bronchiseptica is capable of establishing long-term or chronic infections in swine. Bacterial biofilms are increasingly recognized as important contributors to chronic bacter...

Phytochemicals reduce biofilm formation and inactivates mature biofilm of Campylobacter jejuni

USDA-ARS?s Scientific Manuscript database

Campylobacter jejuni is the leading cause of human foodborne illness globally, and is strongly linked with the consumption of contaminated poultry products. However, little is known about the persistence of C. jejuni in the poultry processing environment. Several studies have shown that C. jejuni ca...

Persistent Themes in Colleges of Business

ERIC Educational Resources Information Center

Bohanon, Cecil E.

2008-01-01

In this article, the author examines a number of issues in colleges of commerce in their formative period from 1900 to 1930. He discusses 4 areas: content of business curriculum, professional nature of business and business schools, social responsibility of corporate managers, and integration of the business curriculum. Many of the topics are…

Distinct Neural Mechanisms Mediate Olfactory Memory Formation at Different Timescales

ERIC Educational Resources Information Center

McNamara, Ann Marie; Magidson, Phillip D.; Linster, Christiane; Wilson, Donald A.; Cleland, Thomas A.

2008-01-01

Habituation is one of the oldest forms of learning, broadly expressed across sensory systems and taxa. Here, we demonstrate that olfactory habituation induced at different timescales (comprising different odor exposure and intertrial interval durations) is mediated by different neural mechanisms. First, the persistence of habituation memory is…

Approximating the Practice of Writing the Dissertation Literature Review

ERIC Educational Resources Information Center

Kucan, Linda

2011-01-01

This article is an attempt to join the conversation about doctoral preparation that has been a persistent but intermittent topic of interest in educational journals. Previous articles have focused on the format of the dissertation, the purpose of the literature review, and approaches to supporting doctoral students in acquiring professional…

Understanding and Addressing the Achievement Gap through Individualized Instruction and Formative Assessment

ERIC Educational Resources Information Center

Yeh, Stuart S.

2010-01-01

This article synthesizes findings regarding the development of competence and learned helplessness and factors influencing persistence and intrinsic motivation, suggests the process through which small differences in early achievement are magnified by the current structure of schools, and reviews evidence suggesting that the characteristics of a…

New geochronologic and palaeomagnetic data for the hominid-bearing Hadar Formation of Ethiopia

USGS Publications Warehouse

Aronson, J.L.; Schmitt, T.J.; Walter, R.C.; Taieb, M.; Tiercelin, J.J.; Johanson, D.C.; Naeser, C.W.; Nairn, A.E.M.

1977-01-01

A 2.6 Myr K/Ar age has been derived for a primary unreworked tuff high in the hominid-bearing Hadar Formation (Kada Hadar Member), stratigraphically above all the important fossil finds. A 2.6 Myr fission track age has been derived on zircons from this tuff. New K/Ar results on the Kadada Moumou basalt (Sidi Hakoma Member) suggest a 3.0 Myr age. Preliminary interpretation of a detailed continuous palaeomagnetic section through the formation indicates the existence of persistent normal and reversed sequences. With the radiometric age control this magnetic sequence appears to correlate with the Gauss Epoch. These initial results imply the fossil-rich Hadar Formation spans from somewhat older than 3.1 Myr to somewhat younger than 2.6 Myr. ?? 1977 Nature Publishing Group.

Epigenetic mechanisms in fear conditioning: Implications for treating post-traumatic stress disorder

PubMed Central

Kwapis, Janine L.; Wood, Marcelo A.

2014-01-01

Post-traumatic stress disorder (PTSD) and other anxiety disorders stemming from dysregulated fear memory are problematic and costly. Understanding the molecular mechanisms that contribute to the formation and maintenance of these persistent fear associations is critical to developing treatments for PTSD. Epigenetic mechanisms, which control gene expression to produce long-lasting changes in cellular function, may support the formation of fear memory underlying PTSD. Here, we address the role of epigenetic mechanisms in the formation, storage, updating, and extinction of fear memories and discuss methods of targeting these epigenetic mechanisms to reduce the initial formation of fear memory or to enhance its extinction. Epigenetic mechanisms may provide a novel target for pharmaceutical and other treatments to reduce aversive memory contributing to PTSD. PMID:25220045

The hormonal and behavioral response to group formation, seasonal changes, and restraint stress in the highly social Malayan Flying Fox (Pteropus vampyrus) and the less social Little Golden-mantled Flying Fox (Pteropus pumilus) (Chiroptera: Pteropodidae).

PubMed

Reeder, DeeAnn M; Kosteczko, Nicole S; Kunz, Thomas H; Widmaier, Eric P

2006-04-01

This study examined behavioral and physiological responses (changes in inter-animal spacing, total glucocorticoids, testosterone, and body mass) to the formation of breeding and same-sex groups in two bat species, the socially gregarious Malayan Flying Fox (Pteropus vampyrus) and the less social Little Golden-mantled Flying Fox (Pteropus pumilus). We hypothesized that social instability, especially in the breeding groups and especially in P. vampyrus, would result in elevated glucocorticoids and that social facilitation of breeding and/or male-male competition would result in persistently higher levels of testosterone in breeding males. Seasonal rhythms in all measures were also predicted, and the glucocorticoid stress response was expected to vary by sex, season, and group type. Nearly all animals responded to group formation with elevated glucocorticoids, but, for breeding animals (especially aggressive male P. vampyrus), these responses persisted over time. In both species, breeding group formation resulted in elevated testosterone in males. Glucocorticoids, testosterone, testes volume, and body mass generally peaked in the breeding season in males (late summer and early autumn), but the seasonal glucocorticoid peak in females occurred in late winter and early spring. All animals responded to restraint stress with elevations in glucocorticoids that largely did not differ by sex, time of year, reproductive condition, group type, or, in lactating females, the presence of her pup. Changes in both behavior and physiology were more evident in P. vampyrus than in P. pumilus, and we believe that their underlying social differences influenced their responses to group formation and to the changing seasonal environment.

Formation of thermal coagulum on multielectrode catheters during phased radiofrequency energy ablation of persistent atrial fibrillation.

PubMed

Debruyne, Philippe; Rossenbacker, Tom; Vankelecom, Bart; Charlier, Filip; Roosen, John; Ector, Bavo; Janssens, Luc

2014-02-01

Radiofrequency ablation (RFA) can unfavorably cause coagulum on the ablation electrode. The aim of this study was to assess this phenomenon on three different multielectrode catheters used to treat persistent atrial fibrillation with duty-cycled RFA. Twenty-six consecutive patients have been treated with the pulmonary vein ablation catheter (PVAC) and the multiarray ablation catheter (MAAC). In 13 patients, additional ablation with the multiarray septal catheter (MASC) has been performed. The multichannel RF generator GENius™ (Medtronic Inc., Minneapolis, MN, USA) independently delivered energy in a bipolar and unipolar mode (ratio of 4/1, 2/1, or 1/1) to any of the electrodes. Versions 14.2, 14.3, and 14.4 of the generator were used. Coagulum presence was determined postablation by careful visual inspection of the catheter electrodes. No coagulum formation was visualized on the PVACs. Coagulum formation was visualized in 59% of the electrodes of the MAACs using a 2/1 mode and the 14.2 software version versus 69% using the 14.4 version and a 2/1 mode (P = 0.7) versus 14% of the electrodes applying a 1/1 ratio and the 14.4 software version (P < 0.001). Duty-cycled RFA in 2/1 bipolar/unipolar ratio generates a substantial frequency of coagulum formation on the multielectrode catheters MAAC and MASC. The use of the 14.4 version of the software to drive the RF generator and the use of energy in the default 1/1 bipolar/unipolar ratio could significantly reduce the frequency of coagulum formation, but so far, could not completely overcome it. The PVAC did not form coagulum, regardless of generator version or energy ratio used. ©2013, The Authors. Journal compilation ©2013 Wiley Periodicals, Inc.

A multiple antibiotic-resistant enterobacter cloacae strain isolated from a bioethanol fermentation facility.

PubMed

Murphree, Colin A; Li, Qing; Heist, E Patrick; Moe, Luke A

2014-09-17

An Enterobacter cloacae strain (E. cloacae F3S3) that was collected as part of a project to assess antibiotic resistance among bacteria isolated from bioethanol fermentation facilities demonstrated high levels of resistance to antibiotics added prophylactically to bioethanol fermentors. PCR assays revealed the presence of canonical genes encoding resistance to penicillin (ampC) and erythromycin (ermG). Assays measuring biofilm formation under antibiotic stress indicated that erythromycin induced biofilm formation in E. cloacae F3S3. Planktonic growth and biofilm formation were observed at a high ethanol content, indicating E. cloacae F3S3 can persist in a bioethanol fermentor under the highly variable environmental conditions found in fermentors.

[Infectious risk related to the formation of multi-species biofilms (Candida - bacteria) on peripheral vascular catheters].

PubMed

Seghir, A; Boucherit-Otmani, Z; Sari-Belkharroubi, L; Boucherit, K

2017-03-01

The Candida yeasts are the fourth leading cause of death from systemic infections, the risk may increase when the infection also involves bacteria. Yeasts and bacteria can adhere to medical implants, such as peripheral vascular catheters, and form a multicellular structures called "mixed biofilms" more resistant to antimicrobials agents. However, the formation of mixed biofilms on implants leads to long-term persistent infections because they can act as reservoirs of pathogens that have poorly understood interactions. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Relationship between seed bank expression, adult longevity and aridity in species of Chaetanthera (Asteraceae) in central Chile.

PubMed

Arroyo, M T K; Chacon, P; Cavieres, L A

2006-09-01

Broad surveys have detected inverse relationships between seed and adult longevity and between seed size and adult longevity. However, low and unpredictable precipitation is also associated with seed bank (SB) expression in semi-arid and arid areas. The relationship between adult longevity, SB formation, seed mass and aridity is examined in annual and perennial herbs of Chaetanthera (Asteraceae) from the Chilean Mediterranean-type climate and winter-rainfall desert areas over a precipitation range of one order of magnitude. Seeds of 18 species and subtaxa (32 populations) were buried in field locations, and exhumed after two successive germination periods. Seeds not germinating in the field were tested in a growth chamber, and remnant intact seed tested for viability. Seed banks were classed as transient or persistent. The effect of life form, species, population and burial time on persistent SB size was assessed with factorial ANOVA. Persistent seed bank size was compared with the Martonne aridity index (shown to be a surrogate for inter-annual variation in precipitation) and seed size using linear regression. ANCOVA assessed the effect of life-form on SB size with aridity as covariate. Three species had a transient SB and 15 a persistent SB. ANOVA revealed a significant effect of life-form on SB size with annuals having larger SB size and greater capacity to form a persistent SB than perennials. Significant inter-population variation in SB size was found in 64% of cases. Seed mass was negatively correlated with persistent SB size. Persistent seed bank size was significantly correlated with the Martonne aridity index in the perennial and annual species, with species from more arid areas having larger persistent SBs. However, when aridity was considered as a covariate, ANCOVA revealed no significant differences between the annual and perennial herbs. Persistent seed bank size in Chaetanthera appears to reflect environmental selection rather than any trade-off with adult longevity.

Persistent gastrocutaneous fistula: factors affecting the need for closure.

PubMed

Wyrick, Deidre L; Bozeman, Andrew P; Smith, Samuel D; Jackson, Richard J; Maxson, R Todd; Kelley, Karen R; Mathews, Donna L; Li, Jingyun; Swearingen, Christopher J; Dassinger, Melvin S

2013-12-01

The occurrence of gastrocutaneous fistula (GCF) is a well-known complication after gastrostomy tube placement. We explore multiple factors to ascertain their impact on the rate of persistent GCF formation. We retrospectively reviewed patient records for all gastrostomies (GT) constructed at our institution from 2007 to 2011. Association of GCF with method of placement, concomitant fundoplication, neurologic findings, duration of therapy, and demographics was evaluated using logistic regression. Nine hundred fifty patients had GTs placed, of which 148 patients had GTs removed and 47 (32%) of 148 required surgical closure secondary to persistent GCF. Laparoscopic and open procedures comprised 79 (53%) of 148 and 69 (47%) of 148, respectively. Seventeen (22%) patients in the laparoscopic group developed persistent GCF, compared to 30 (43%) in the open group (P=0.035, OR=2.52). Seventy-one patients had concomitant Nissen fundoplication. Thirty-one (44%) developed GCF, compared to 16 (21%) without a Nissen (P=0.002, OR=4.94). Patients with button in place for 303 days had persistent GCF incidence of 23%, compared to 45% at 540 days (P<0.001, OR=3.51) and 50% at 850 days (P=0.011, OR=4.51). Patients with device placed at 1.8 months of age were more likely to develop GCF compared to those with device placed at 8.9 months of age (P=0.017, OR=2.35). Open operations, concurrent Nissen and younger age at placement were all statistically significant factors causing persistent GCF. © 2013.

Application of a Persistent Dissolved-phase Reactive Treatment Zone for Mitigation of Mass Discharge from Sources Located in Lower-Permeability Sediments

PubMed Central

Marble, J.C.; Brusseau, M.L.; Carroll, K.C.; Plaschke, M.; Fuhrig, L.; Brinker, F.

2015-01-01

The purpose of this study is to examine the development and effectiveness of a persistent dissolved-phase treatment zone, created by injecting potassium permanganate solution, for mitigating discharge of contaminant from a source zone located in a relatively deep, low-permeability formation. A localized 1,1-dichloroethene (DCE) source zone comprising dissolved- and sorbed-phase mass is present in lower permeability strata adjacent to a sand/gravel unit in a section of the Tucson International Airport Area (TIAA) Superfund Site. The results of bench-scale studies conducted using core material collected from boreholes drilled at the site indicated that natural oxidant demand was low, which would promote permanganate persistence. The reactive zone was created by injecting a permanganate solution into multiple wells screened across the interface between the lower-permeability and higher-permeability units. The site has been monitored for nine years to characterize the spatial distribution of DCE and permanganate. Permanganate continues to persist at the site, and a substantial and sustained decrease in DCE concentrations in groundwater has occurred after the permanganate injection.. These results demonstrate successful creation of a long-term, dissolved-phase reactive-treatment zone that reduced mass discharge from the source. This project illustrates the application of in-situ chemical oxidation as a persistent dissolved-phase reactive-treatment system for lower-permeability source zones, which appears to effectively mitigate persistent mass discharge into groundwater. PMID:26300570

Defining ADHD symptom persistence in adulthood: optimizing sensitivity and specificity.

PubMed

Sibley, Margaret H; Swanson, James M; Arnold, L Eugene; Hechtman, Lily T; Owens, Elizabeth B; Stehli, Annamarie; Abikoff, Howard; Hinshaw, Stephen P; Molina, Brooke S G; Mitchell, John T; Jensen, Peter S; Howard, Andrea L; Lakes, Kimberley D; Pelham, William E

2017-06-01

Longitudinal studies of children diagnosed with ADHD report widely ranging ADHD persistence rates in adulthood (5-75%). This study documents how information source (parent vs. self-report), method (rating scale vs. interview), and symptom threshold (DSM vs. norm-based) influence reported ADHD persistence rates in adulthood. Five hundred seventy-nine children were diagnosed with DSM-IV ADHD-Combined Type at baseline (ages 7.0-9.9 years) 289 classmates served as a local normative comparison group (LNCG), 476 and 241 of whom respectively were evaluated in adulthood (Mean Age = 24.7). Parent and self-reports of symptoms and impairment on rating scales and structured interviews were used to investigate ADHD persistence in adulthood. Persistence rates were higher when using parent rather than self-reports, structured interviews rather than rating scales (for self-report but not parent report), and a norm-based (NB) threshold of 4 symptoms rather than DSM criteria. Receiver-Operating Characteristics (ROC) analyses revealed that sensitivity and specificity were optimized by combining parent and self-reports on a rating scale and applying a NB threshold. The interview format optimizes young adult self-reporting when parent reports are not available. However, the combination of parent and self-reports from rating scales, using an 'or' rule and a NB threshold optimized the balance between sensitivity and specificity. With this definition, 60% of the ADHD group demonstrated symptom persistence and 41% met both symptom and impairment criteria in adulthood. © 2016 Association for Child and Adolescent Mental Health.

Social Memory Formation Rapidly and Differentially Affects the Motivation and Performance of Vocal Communication Signals in the Bengalese Finch (Lonchura striata var. domestica).

PubMed

Toccalino, Danielle C; Sun, Herie; Sakata, Jon T

2016-01-01

Cognitive processes like the formation of social memories can shape the nature of social interactions between conspecifics. Male songbirds use vocal signals during courtship interactions with females, but the degree to which social memory and familiarity influences the likelihood and structure of male courtship song remains largely unknown. Using a habituation-dishabituation paradigm, we found that a single, brief (<30 s) exposure to a female led to the formation of a short-term memory for that female: adult male Bengalese finches were significantly less likely to produce courtship song to an individual female when re-exposed to her 5 min later (i.e., habituation). Familiarity also rapidly decreased the duration of courtship songs but did not affect other measures of song performance (e.g., song tempo and the stereotypy of syllable structure and sequencing). Consistent with a contribution of social memory to the decrease in courtship song with repeated exposures to the same female, the likelihood that male Bengalese finches produced courtship song increased when they were exposed to a different female (i.e., dishabituation). Three consecutive exposures to individual females also led to the formation of a longer-term memory that persisted over days. Specifically, when courtship song production was assessed 2 days after initial exposures to females, males produced fewer and shorter courtship songs to familiar females than to unfamiliar females. Measures of song performance, however, were not different between courtship songs produced to familiar and unfamiliar females. The formation of a longer-term memory for individual females seemed to require at least three exposures because males did not differentially produce courtship song to unfamiliar females and females that they had been exposed to only once or twice. Taken together, these data indicate that brief exposures to individual females led to the rapid formation and persistence of social memories and support the existence of distinct mechanisms underlying the motivation to produce and the performance of courtship song.

Adherence of curli producing Shiga-toxigenic Escherichia coli to baby spinach leaves

USDA-ARS?s Scientific Manuscript database

Cellular appendages, such as curli fibers have been suggested to be involved in STEC persistence in fresh produce as these curli are critical in biofilm formation and adherence to animal cells. We determined the role of curli in attachment of STEC on spinach leaves. The curli expression by wild-ty...

Preliminary Success and Retention Rates in Selected Math Courses. Research Report.

ERIC Educational Resources Information Center

Cuesta Coll., San Luis Obispo, CA. Matriculation and Research Services.

This report presents findings of exploratory research on success, retention, and persistence in math courses at Cuesta College. The following research questions were addressed: (1) How do success rates in Math 23 (elementary algebra) and Math 27 (intermediate algebra) compare with traditional and computer-assisted formats? (2) What are the…

International Distance Learning in Special Education: A Program Evaluation of a US-Ecuador Collaboration

ERIC Educational Resources Information Center

McPherson, Rebekah

2010-01-01

The internationalization of distance learning in special education is at a pivotal point in expansion. Even with concerted efforts through traditional means to increase the supply of special educators, shortages persist; therefore, teacher preparation programs are turning to online education. This dissertation study was a formative program…

FORMATION OF NITRO MUSK ADDUCTS OF RAINBOW TROUT HEMOGLOBIN FOR POTENTIAL USE AS BIOMARKERS OF EXPOSURE

EPA Science Inventory

The high use of nitro musk xylene (MX) and musk ketone (MK) as fragrances, and their persistence and bioaccumulation potential make them ubiquitous environmental contaminants. The 4-amino-MX (AMX) and 2-amino-MK (AMK) metabolites have been detected in trout fish hemoglobin (Hb) s...

Detection and Learning of Unexpected Behaviors of Systems of Dynamical Systems by Using the Q2 Abstractions

DTIC Science & Technology

2017-11-01

Finite State Machine ............................................... 21 9 Main Ontological Concepts for Representing Structure of a Multi -Agent...19 NetLogo Simulation of persistent surveillance of circular plume by 4 UAVs ........................36 20 Flocking Emergent Behaviors in Multi -UAV...Region) - Undesirable Group Formation ................................................................................... 40 24 Two UAVs Moving in

Molecular analysis of vector genome structures after liver transduction by conventional and self-complementary adeno-associated viral serotype vectors in murine and nonhuman primate models.

PubMed

Sun, Xun; Lu, You; Bish, Lawrence T; Calcedo, Roberto; Wilson, James M; Gao, Guangping

2010-06-01

Vectors based on several new adeno-associated viral (AAV) serotypes demonstrated strong hepatocyte tropism and transduction efficiency in both small- and large-animal models for liver-directed gene transfer. Efficiency of liver transduction by AAV vectors can be further improved in both murine and nonhuman primate (NHP) animals when the vector genomes are packaged in a self-complementary (sc) format. In an attempt to understand potential molecular mechanism(s) responsible for enhanced transduction efficiency of the sc vector in liver, we performed extensive molecular studies of genome structures of conventional single-stranded (ss) and sc AAV vectors from liver after AAV gene transfer in both mice and NHPs. These included treatment with exonucleases with specific substrate preferences, single-cutter restriction enzyme digestion and polarity-specific hybridization-based vector genome mapping, and bacteriophage phi29 DNA polymerase-mediated and double-stranded circular template-specific rescue of persisted circular genomes. In mouse liver, vector genomes of both genome formats seemed to persist primarily as episomal circular forms, but sc vectors converted into circular forms more rapidly and efficiently. However, the overall differences in vector genome abundance and structure in the liver between ss and sc vectors could not account for the remarkable differences in transduction. Molecular structures of persistent genomes of both ss and sc vectors were significantly more heterogeneous in macaque liver, with noticeable structural rearrangements that warrant further characterizations.

Molecular Analysis of Vector Genome Structures After Liver Transduction by Conventional and Self-Complementary Adeno-Associated Viral Serotype Vectors in Murine and Nonhuman Primate Models

PubMed Central

Sun, Xun; Lu, You; Bish, Lawrence T.; Calcedo, Roberto; Wilson, James M.

2010-01-01

Abstract Vectors based on several new adeno-associated viral (AAV) serotypes demonstrated strong hepatocyte tropism and transduction efficiency in both small- and large-animal models for liver-directed gene transfer. Efficiency of liver transduction by AAV vectors can be further improved in both murine and nonhuman primate (NHP) animals when the vector genomes are packaged in a self-complementary (sc) format. In an attempt to understand potential molecular mechanism(s) responsible for enhanced transduction efficiency of the sc vector in liver, we performed extensive molecular studies of genome structures of conventional single-stranded (ss) and sc AAV vectors from liver after AAV gene transfer in both mice and NHPs. These included treatment with exonucleases with specific substrate preferences, single-cutter restriction enzyme digestion and polarity-specific hybridization-based vector genome mapping, and bacteriophage ϕ29 DNA polymerase-mediated and double-stranded circular template-specific rescue of persisted circular genomes. In mouse liver, vector genomes of both genome formats seemed to persist primarily as episomal circular forms, but sc vectors converted into circular forms more rapidly and efficiently. However, the overall differences in vector genome abundance and structure in the liver between ss and sc vectors could not account for the remarkable differences in transduction. Molecular structures of persistent genomes of both ss and sc vectors were significantly more heterogeneous in macaque liver, with noticeable structural rearrangements that warrant further characterizations. PMID:20113166

The persistent microbicidal effect in water exposed to the corona discharge.

PubMed

Julák, Jaroslav; Scholtz, Vladimír; Kotúčová, Soňa; Janoušková, Olga

2012-07-01

This article describes and particularly explains a new phenomenon of persistent microbicidal effect of water previously exposed to the low-temperature plasma, which cannot be attributed to the acidification only. The direct microbicidal action of plasma is well documented, being mediated by number of reactive particles with a short lifetime. However, we observed the microbicidal effect also in exposed water stored for a month, where it must be mediated by stable particles. In water and in phosphate-buffered saline, the formation of NO(x) and corresponding acids, H(2)O(2) and O(3) was confirmed after exposition to the low-temperature plasma generated in air by DC negative glow corona and positive streamer discharge. The time course of acidification, H(2)O(2) and O(3) formation were deremined. Except uncertain traces of HCN, SIFT-MS analysis of exposed liquids reveals no additional reactive compounds. The microbicidal effect persists almost unchanged during 4 weeks of storage, although O(3) completely and H(2)O(2) almost disappears. Staphylococcus epidermidis and Escherichia coli were inactivated within 10 min of incubation in exposed liquids, Candida albicans needs at least 1 h. The solutions prepared by artificial mixing of reactive compounds mimic the action of exposed water, but in lesser extent. The acid milieu is the main cause of the microbicidal effect, but the possibility of still unidentified additional compound remains open. Copyright © 2011 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Differential role of calpain-dependent protein cleavage in intermediate and long-term operant memory in Aplysia.

PubMed

Lyons, Lisa C; Gardner, Jacob S; Lentsch, Cassidy T; Gandour, Catherine E; Krishnan, Harini C; Noakes, Eric J

2017-01-01

In addition to protein synthesis, protein degradation or protein cleavage may be necessary for intermediate (ITM) and long-term memory (LTM) to remove molecular constraints, facilitate persistent kinase activity and modulate synaptic plasticity. Calpains, a family of conserved calcium dependent cysteine proteases, modulate synaptic function through protein cleavage. We used the marine mollusk Aplysia californica to investigate the in vivo role of calpains during intermediate and long-term operant memory formation using the learning that food is inedible (LFI) paradigm. A single LFI training session, in which the animal associates a specific netted seaweed with the failure to swallow, generates short (30min), intermediate (4-6h) and long-term (24h) memory. Using the calpain inhibitors calpeptin and MDL-28170, we found that ITM requires calpain activity for induction and consolidation similar to the previously reported requirements for persistent protein kinase C activity in intermediate-term LFI memory. The induction of LTM also required calpain activity. In contrast to ITM, calpain activity was not necessary for the molecular consolidation of LTM. Surprisingly, six hours after LFI training we found that calpain activity was necessary for LTM, although this is a time at which neither persistent PKC activity nor protein synthesis is required for the maintenance of long-term LFI memory. These results demonstrate that calpains function in multiple roles in vivo during associative memory formation. Copyright © 2016 Elsevier Inc. All rights reserved.

14C tebuconazole degradation in Colombian soils.

PubMed

Mosquera, C S; Martínez, M J; Guerrero, J A

2010-01-01

Tebuconazole is a fungicide used on onion crops (Allium Fistulosum L) in Colombia. Persistence of pesticides in soils is characterized by the half-life (DT50), which is influenced by their chemical structure, the physical and chemical properties of the soil and the previous soil history. Based on its structural and chemical properties, tebuconazole should be expected to be relatively persistent in soils. Laboratory incubation studies were conducted to evaluate persistence and bond residues of 14C tebuconazole in three soils, two inceptisol (I) and one histosol (H). Textural classifications were: loam (101), loamy sand (102) and loam (H03), respectively. Data obtained followed a first-order degradation kinetics (R2 > or = 0.899) with DT50 values between 158 and 198 days. The production of 14CO2 from the 14C-ring-labelled test chemicals was very low and increased slightly during 63 days in all cases. The methanol extractable 14C-residues were higher than aqueous ones and both decreased over incubation time for the three soils. The formation of bound 14C-residues increased with time and final values were 11.3; 5.55 and 7.87% for 101, 102 and H03 respectively. Soil 101 showed the lowest mineralization rate and the highest bound residues formation, which might be explained by the clay fraction content. In contrast, an inverse behavior was found for soils 102 and H03, these results might be explained by the higher soil organic carbon content.

Persistence of the cell-cycle checkpoint kinase Wee1 in SadA- and SadB-deficient neurons disrupts neuronal polarity.

PubMed

Müller, Myriam; Lutter, Daniela; Püschel, Andreas W

2010-01-15

Wee1 is well characterized as a cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells. Here we identify a novel function of Wee1 in postmitotic neurons during the establishment of distinct axonal and dendritic compartments, which is an essential step during neuronal development. Wee1 is expressed in unpolarized neurons but is downregulated after neurons have extended an axon. Suppression of Wee1 impairs the formation of minor neurites but does not interfere with axon formation. However, neuronal polarity is disrupted when neurons fail to downregulate Wee1. The kinases SadA and SadB (Sad kinases) phosphorylate Wee1 and are required to initiate its downregulation in polarized neurons. Wee1 expression persists in neurons that are deficient in SadA and SadB and disrupts neuronal polarity. Knockdown of Wee1 rescues the Sada(-/-);Sadb(-/-) mutant phenotype and restores normal polarity in these neurons. Our results demonstrate that the regulation of Wee1 by SadA and SadB kinases is essential for the differentiation of polarized neurons.

Significance of ambient conditions in uranium absorption and emission features of laser ablation plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Skrodzki, P. J.; Shah, N. P.; Taylor, N.

2016-11-01

This study employs laser ablation (LA) to investigate mechanisms for U optical signal variation under various environmental conditions during laser absorption spectroscopy (LAS) and optical emission spectroscopy (OES). Potential explored mechanisms for signal quenching related to ambient conditions include plasma chemistry (e.g., uranium oxide formation), ambient gas confinement effects, and other collisional interactions between plasma constituents and the ambient gas. LA-LAS studies show that the persistence of the U ground state population is significantly reduced in the presence of air ambient compared to nitrogen. LA-OES results yield congested spectra from which the U I 356.18 nm transition is prominent andmore » serves as the basis for signal tracking. LA-OES signal and persistence vary negligibly between the test gases (air and N2), unlike the LA-LAS results. The plume hydrodynamic features and plume fundamental properties showed similar results in both air and nitrogen ambient. Investigation of U oxide formation in the laser-produced plasma suggests that low U concentration in a sample hinders consistent detection of UO molecular spectra.« less

Significance of ambient conditions in uranium absorption and emission features of laser ablation plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Skrodzki, P. J.; Shah, N. P.; Taylor, N.

2016-10-02

This study employs laser ablation (LA) to investigate mechanisms for U optical signal variation under various environmental conditions during laser absorption spectroscopy (LAS) and optical emission spectroscopy (OES). Potential mechanisms explored for signal quenching related to ambient conditions include plasma chemistry (e.g., uranium oxide formation), ambient gas confinement effects, and other collisional interactions between plas-ma constituents and the ambient gas. LA-LAS studies show that the persistence of the U ground state population is significantly reduced in the presence of air ambient compared to nitrogen. LA-OES yields congested spectra from which the U I 356.18 nm transition is prominent and servesmore » as the basis for signal tracking. LA-OES signal and per-sistence vary negligibly between the test gases (air and N 2), unlike the LA-LAS results. The plume hydrodynamic features and plume fundamental properties showed similar results in both air and nitrogen ambient. In conclusion, investigation of U oxide formation in the laser-produced plasma suggests that low U concentration in a sample hinders consistent detection of UO molecular spectra.« less

Galaxy evolution in groups and clusters: star formation rates, red sequence fractions and the persistent bimodality

NASA Astrophysics Data System (ADS)

Wetzel, Andrew R.; Tinker, Jeremy L.; Conroy, Charlie

2012-07-01

Using galaxy group/cluster catalogues created from the Sloan Digital Sky Survey Data Release 7, we examine in detail the specific star formation rate (SSFR) distribution of satellite galaxies and its dependence on stellar mass, host halo mass and halo-centric radius. All galaxies, regardless of central satellite designation, exhibit a similar bimodal SSFR distribution, with a strong break at SSFR ≈ 10-11 yr-1 and the same high SSFR peak; in no regime is there ever an excess of galaxies in the 'green valley'. Satellite galaxies are simply more likely to lie on the quenched ('red sequence') side of the SSFR distribution. Furthermore, the satellite quenched fraction excess above the field galaxy value is nearly independent of galaxy stellar mass. An enhanced quenched fraction for satellites persists in groups with halo masses down to 3 × 1011 M⊙ and increases strongly with halo mass and towards halo centre. We find no detectable quenching enhancement for galaxies beyond ˜2 Rvir around massive clusters once these galaxies have been decomposed into centrals and satellites. These trends imply that (1) galaxies experience no significant environmental effects until they cross within ˜Rvir of a more massive host halo; (2) after this, star formation in active satellites continues to evolve in the same manner as active central galaxies for several Gyr; and (3) once begun, satellite star formation quenching occurs rapidly. These results place strong constraints on satellite-specific quenching mechanisms, as we will discuss further in companion papers.

Generation of Covalently Closed Circular DNA of Hepatitis B Viruses via Intracellular Recycling Is Regulated in a Virus Specific Manner

PubMed Central

Köck, Josef; Rösler, Christine; Zhang, Jing-Jing; Blum, Hubert E.; Nassal, Michael; Thoma, Christian

2010-01-01

Persistence of hepatitis B virus (HBV) infection requires covalently closed circular (ccc)DNA formation and amplification, which can occur via intracellular recycling of the viral polymerase-linked relaxed circular (rc) DNA genomes present in virions. Here we reveal a fundamental difference between HBV and the related duck hepatitis B virus (DHBV) in the recycling mechanism. Direct comparison of HBV and DHBV cccDNA amplification in cross-species transfection experiments showed that, in the same human cell background, DHBV but not HBV rcDNA converts efficiently into cccDNA. By characterizing the distinct forms of HBV and DHBV rcDNA accumulating in the cells we find that nuclear import, complete versus partial release from the capsid and complete versus partial removal of the covalently bound polymerase contribute to limiting HBV cccDNA formation; particularly, we identify genome region-selectively opened nuclear capsids as a putative novel HBV uncoating intermediate. However, the presence in the nucleus of around 40% of completely uncoated rcDNA that lacks most if not all of the covalently bound protein strongly suggests a major block further downstream that operates in the HBV but not DHBV recycling pathway. In summary, our results uncover an unexpected contribution of the virus to cccDNA formation that might help to better understand the persistence of HBV infection. Moreover, efficient DHBV cccDNA formation in human hepatoma cells should greatly facilitate experimental identification, and possibly inhibition, of the human cell factors involved in the process. PMID:20824087

Identification of Salmonella enterica Serovar Typhimurium Genes Regulated during Biofilm Formation on Cholesterol Gallstone Surfaces

PubMed Central

Gonzalez-Escobedo, Geoffrey

2013-01-01

Salmonella spp. are able to form biofilms on abiotic and biotic surfaces. In vivo studies in our laboratory have shown that Salmonella can form biofilms on the surfaces of cholesterol gallstones in the gallbladders of mice and human carriers. Biofilm formation on gallstones has been demonstrated to be a mechanism of persistence. The purpose of this work was to identify and evaluate Salmonella sp. cholesterol-dependent biofilm factors. Differential gene expression analysis between biofilms on glass or cholesterol-coated surfaces and subsequent quantitative real-time PCR (qRT-PCR) revealed that type 1 fimbria structural genes and a gene encoding a putative outer membrane protein (ycfR) were specifically upregulated in Salmonella enterica serovar Typhimurium biofilms grown on cholesterol-coated surfaces. Spatiotemporal expression of ycfR and FimA verified their regulation during biofilm development on cholesterol-coated surfaces. Surprisingly, confocal and scanning electron microscopy demonstrated that a mutant of type 1 fimbria structural genes (ΔfimAICDHF) and a ycfR mutant showed increased biofilm formation on cholesterol-coated surfaces. In vivo experiments using Nramp1+/+ mice harboring gallstones showed that only the ΔycfR mutant formed extensive biofilms on mouse gallstones at 7 and 21 days postinfection; ΔfimAICDHF was not observed on gallstone surfaces after the 7-day-postinfection time point. These data suggest that in Salmonella spp., wild-type type 1 fimbriae are important for attachment to and/or persistence on gallstones at later points of chronic infection, whereas YcfR may represent a specific potential natural inhibitor of initial biofilm formation on gallstones. PMID:23897604

The Valley Networks on Mars

NASA Astrophysics Data System (ADS)

Gulick, V. C.

2002-12-01

Despite three decades of exploration, the valley networks on Mars still seem to raise more questions than they answer. Valley systems have formed in the southern highlands, along some regions of the dichotomy boundary and the south rim of Valles Marineris, around the rim of some impact craters, and on the flanks of some volcanoes. They are found on some of the oldest and youngest terrains as well as on intermediate aged surfaces. There is surprisingly little consensus as to the formation and the paleoclimatic implications of the valley networks. Did the valleys require a persistent solar-driven atmospheric hydrological cycle involving precipitation, surface runoff, infiltration and groundwater outflow as they typically do on Earth? Or are they the result of magmatic or impact-driven thermal cycling of ground water involving persistent outflow and subsequent runoff? Are they the result of some other process(es)? Ground-water sapping, surface-water runoff, debris flows, wind erosion, and formation mechanisms involving other fluids have been proposed. Until such basic questions as these are definitively answered, their significance for understanding paleoclimatic change on Mars remains cloudy. I will review what is known about valley networks using data from both past and current missions. I will discuss what we have learned about their morphology, environments in which they formed, their spatial and temporal associations, possible formation mechanisms, relation to outflow channel and gully formation, as well as the possible implications for past climate change on Mars. Finally I will discuss how future, meter to submeter scale imaging and other remote sensing observations may shed new light on the debate over the origin of these enigmatic features.

Persistent Charge-Density-Wave Order in Single-Layer TaSe2.

PubMed

Ryu, Hyejin; Chen, Yi; Kim, Heejung; Tsai, Hsin-Zon; Tang, Shujie; Jiang, Juan; Liou, Franklin; Kahn, Salman; Jia, Caihong; Omrani, Arash A; Shim, Ji Hoon; Hussain, Zahid; Shen, Zhi-Xun; Kim, Kyoo; Min, Byung Il; Hwang, Choongyu; Crommie, Michael F; Mo, Sung-Kwan

2018-02-14

We present the electronic characterization of single-layer 1H-TaSe 2 grown by molecular beam epitaxy using a combined angle-resolved photoemission spectroscopy, scanning tunneling microscopy/spectroscopy, and density functional theory calculations. We demonstrate that 3 × 3 charge-density-wave (CDW) order persists despite distinct changes in the low energy electronic structure highlighted by the reduction in the number of bands crossing the Fermi energy and the corresponding modification of Fermi surface topology. Enhanced spin-orbit coupling and lattice distortion in the single-layer play a crucial role in the formation of CDW order. Our findings provide a deeper understanding of the nature of CDW order in the two-dimensional limit.

Habitat restoration promotes pollinator persistence and colonization in intensively managed agriculture.

PubMed

M'Gonigle, Leithen K; Ponisio, Lauren C; Cutler, Kerry; Kremen, Claire

2015-09-01

Widespread evidence of pollinator declines has led to policies supporting habitat restoration including in agricultural landscapes. Yet, little is yet known about the effectiveness of these restoration techniques for promoting stable populations and communities of pollinators, especially in intensively managed agricultural landscapes. Introducing floral resources, such as flowering hedgerows, to enhance intensively cultivated agricultural landscapes is known to increase the abundances of native insect pollinators in and around restored areas. Whether this is a result of local short-term concentration at flowers or indicative of true increases in the persistence and species richness of these communities remains unclear. It is also unknown whether this practice supports species of conservation concern (e.g., those with more specialized dietary requirements). Analyzing occupancies of native bees and syrphid flies from 330 surveys across 15 sites over eight years, we found that hedgerow restoration promotes rates of between-season persistence and colonization as compared with unrestored field edges. Enhanced persistence and colonization, in turn, led to the formation of more species-rich communities. We also find that hedgerows benefit floral resource specialists more than generalists, emphasizing the value of this restoration technique for conservation in agricultural landscapes.

Intraseptal infusion of oxotremorine impairs memory in a delayed-non-match-to-sample radial maze task.

PubMed

Bunce, J G; Sabolek, H R; Chrobak, J J

2003-01-01

The medial septal nucleus is part of the forebrain circuitry that supports memory. This nucleus is rich in cholinergic receptors and is a putative target for the development of cholinomimetic cognitive-enhancing drugs. Septal neurons, primarily cholinergic and GABAergic, innervate the entire hippocampal formation and regulate hippocampal formation physiology and emergent function. Direct intraseptal drug infusions can produce amnestic or promnestic effects depending upon the type of drug administered. However, intraseptal infusion of the cholinomimetic oxotremorine has been reported to produce both promnestic and amnestic effects when administered prior to task performance. The present study examined whether post-acquisition intraseptal infusion of oxotremorine would be promnestic or amnestic in a delayed-non-match-to-sample radial maze task. In this task rats must remember information about spatial locations visited during a daily sample session and maintain that information over extended retention intervals (hours) in order to perform accurately on the daily test session. Treatments may then be administered during the retention interval. Alterations in maze performance during the test session an hour or more after treatment evidences effects on memory. In the present study, intraseptal infusion of oxotremorine (1.0-10.0 microg) produced a linear dose-related impairment of memory performance. Importantly, we also observed disrupted performance on the day after treatment. This persistent deficit was related only to memory over the retention interval and did not affect indices of short-term memory (ability to avoid repetitive or proactive errors during both the pre- and post-delay sessions). The persistent deficit contrasts with the acute amnestic effects of other intraseptally administered drugs including the cholinomimetics carbachol and tacrine. Thus, intraseptal oxotremorine produced a preferential disruption of memory consolidation as well as a persistent alteration of medial septal circuits. These findings are discussed with regards to multi-stage models of hippocampal-dependent memory formation and the further development of therapeutic strategies in the treatment of mild cognitive impairment as well as age-related decline and Alzheimer's dementia.

Flow-sediment-large woody debris interplay: Introducing an appropriately scaled laboratory experiment

NASA Astrophysics Data System (ADS)

Friedrich, H.; Spreitzer, G.; Tunnicliffe, J. F.

2017-12-01

The morphology of steep (>0.01 m/m) forested streams is governed not only by water-sediment interplay, but also by accumulations of coarse and fine organic debris. In this project we look at the jamming dynamics (formation, persistence and hydraulic feedbacks) of large woody debris with the help of scaled laboratory experiments. In New Zealand, the recruitment of wood from both natural tree-fall and forest harvesting has led to obstruction of culverts, bridges and other river constrictions. Understanding the dynamics of jam formation and persistence is important for harvest practice guidelines, management of sediment accumulation, as well as establishing impacts to habitat and infrastructure. In this study, we provide the context of our work, present our experimental setup for studying the complex flow-sediment-wood interactions and present some initial results. In our experimental setup, we varied feed rates of sediment and organic fine material in order to establish concentration thresholds for jam formation, and development of sediment retention capacity upstream of the jam. Large woody debris accumulation is studied for different blocking scenarios, and the effect on sediment transport is measured. Sediment quantities and changes in channel bed morphology upstream of the critical cross section are evaluated, together with resulting backwater effects, and associated energy losses. In the long term, our results will inform our understanding of the processes that take place from the mobilization of woody debris to accumulation.

Catechol degradation on hematite/silica-gas interface as affected by gas composition and the formation of environmentally persistent free radicals.

PubMed

Li, Hao; Guo, Huiying; Pan, Bo; Liao, Shaohua; Zhang, Di; Yang, Xikun; Min, Chungang; Xing, Baoshan

2016-04-15

Environmentally persistent free radicals (EPFRs) formed on a solid particle surface have received increasing attention because of their toxic effects. However, organic chemical fate regulated by EPFRs has rarely been investigated, and this information may provide the missing link in understanding their environmental behavior. Previous studies have suggested that the reduction of transition metals is involved in EPFRs formation. We thus hypothesize that an oxidative environment may inhibit EPFRs formation in particle-gas interface, which will consequently release free radicals and accelerate organic chemical degradation. Our result indicates that a 1% hematite coating on a silica surface inhibited catechol degradation in N2, especially at low catechol loadings on solid particles (SCT). However, under an O2 environment, catechol degradation decreased when SCT was <1 μg/mg but increased when SCT was >1 μg/mg. Stable organic free radicals were observed in the N2 system with g factors in the 2.0035-2.0050 range, suggesting the dominance of oxygen-centered free radicals. The introduction of O2 into the catechol degradation system substantially decreased the free radical signals and decreased the Fe(II) content. These results were observed in both dark and light irradiation systems, indicating the ubiquitous presence of EPFRs in regulating the fate of organic chemicals.

The relationship between PSD-95 clustering and spine stability in vivo.

PubMed

Cane, Michele; Maco, Bohumil; Knott, Graham; Holtmaat, Anthony

2014-02-05

The appearance and disappearance of dendritic spines, accompanied by synapse formation and elimination may underlie the experience-dependent reorganization of cortical circuits. The exact temporal relationship between spine and synapse formation in vivo remains unclear, as does the extent to which synapse formation enhances the stability of newly formed spines and whether transient spines produce synapses. We used in utero electroporation of DsRedExpress- and eGFP-tagged postsynaptic density protein 95 (PSD-95) to investigate the relationship between spine and PSD stability in mouse neocortical L2/3 pyramidal cells in vivo. Similar to previous studies, spines and synapses appeared and disappeared, even in naive animals. Cytosolic spine volumes and PSD-95-eGFP levels in spines covaried over time, suggesting that the strength of many individual synapses continuously changes in the adult neocortex. The minority of newly formed spines acquired PSD-95-eGFP puncta. Spines that failed to acquire a PSD rarely survived for more than a day. Although PSD-95-eGFP accumulation was associated with increased spine lifetimes, most new spines with a PSD did not convert into persistent spines. This indicates that transient spines may serve to produce short-lived synaptic contacts. Persistent spines that were destined to disappear showed, on average, reduced PSD-95-eGFP levels well before the actual pruning event. Altogether, our data indicate that the PSD size relates to spine stability in vivo.

Catechol degradation on hematite/silica–gas interface as affected by gas composition and the formation of environmentally persistent free radicals

PubMed Central

Li, Hao; Guo, Huiying; Pan, Bo; Liao, Shaohua; Zhang, Di; Yang, Xikun; Min, Chungang; Xing, Baoshan

2016-01-01

Environmentally persistent free radicals (EPFRs) formed on a solid particle surface have received increasing attention because of their toxic effects. However, organic chemical fate regulated by EPFRs has rarely been investigated, and this information may provide the missing link in understanding their environmental behavior. Previous studies have suggested that the reduction of transition metals is involved in EPFRs formation. We thus hypothesize that an oxidative environment may inhibit EPFRs formation in particle-gas interface, which will consequently release free radicals and accelerate organic chemical degradation. Our result indicates that a 1% hematite coating on a silica surface inhibited catechol degradation in N2, especially at low catechol loadings on solid particles (SCT). However, under an O2 environment, catechol degradation decreased when SCT was <1 μg/mg but increased when SCT was >1 μg/mg. Stable organic free radicals were observed in the N2 system with g factors in the 2.0035–2.0050 range, suggesting the dominance of oxygen-centered free radicals. The introduction of O2 into the catechol degradation system substantially decreased the free radical signals and decreased the Fe(II) content. These results were observed in both dark and light irradiation systems, indicating the ubiquitous presence of EPFRs in regulating the fate of organic chemicals. PMID:27079263

Adaptation to Lactose in Lactase Non Persistent People: Effects on Intolerance and the Relationship between Dairy Food Consumption and Evalution of Diseases.

PubMed

Szilagyi, Andrew

2015-08-13

Dairy foods contain complex nutrients which interact with the host. Yet, evolution of lactase persistence has divided the human species into those that can or cannot digest lactose in adulthood. Such a ubiquitous trait has differential effects on humanity. The literature is reviewed to explore how the divide affects lactose handling by lactase non persistent persons. There are two basic differences in digesters. Firstly, maldigesters consume less dairy foods, and secondly, excess lactose is digested by colonic microflora. Lactose intolerance in maldigesters may occur with random lactose ingestion. However, lactose intolerance without maldigestion tends to detract from gaining a clear understanding of the mechanisms of symptoms formation and leads to confusion with regards to dairy food consumption. The main consequence of intolerance is withholding dairy foods. However, regular dairy food consumption by lactase non persistent people could lead to colonic adaptation by the microbiome. This process may mimic a prebiotic effect and allows lactase non persistent people to consume more dairy foods enhancing a favorable microbiome. This process then could lead to alterations in outcome of diseases in response to dairy foods in lactose maldigesters. The evidence that lactose is a selective human prebiotic is reviewed and current links between dairy foods and some diseases are discussed within this context. Colonic adaptation has not been adequately studied, especially with modern microbiological techniques.

Adaptation to Lactose in Lactase Non Persistent People: Effects on Intolerance and the Relationship between Dairy Food Consumption and Evalution of Diseases

PubMed Central

Szilagyi, Andrew

2015-01-01

Dairy foods contain complex nutrients which interact with the host. Yet, evolution of lactase persistence has divided the human species into those that can or cannot digest lactose in adulthood. Such a ubiquitous trait has differential effects on humanity. The literature is reviewed to explore how the divide affects lactose handling by lactase non persistent persons. There are two basic differences in digesters. Firstly, maldigesters consume less dairy foods, and secondly, excess lactose is digested by colonic microflora. Lactose intolerance in maldigesters may occur with random lactose ingestion. However, lactose intolerance without maldigestion tends to detract from gaining a clear understanding of the mechanisms of symptoms formation and leads to confusion with regards to dairy food consumption. The main consequence of intolerance is withholding dairy foods. However, regular dairy food consumption by lactase non persistent people could lead to colonic adaptation by the microbiome. This process may mimic a prebiotic effect and allows lactase non persistent people to consume more dairy foods enhancing a favorable microbiome. This process then could lead to alterations in outcome of diseases in response to dairy foods in lactose maldigesters. The evidence that lactose is a selective human prebiotic is reviewed and current links between dairy foods and some diseases are discussed within this context. Colonic adaptation has not been adequately studied, especially with modern microbiological techniques. PMID:26287234

The Enigmatic Persistence of Anorexia Nervosa

PubMed Central

Walsh, B. Timothy

2014-01-01

Objective In this review, based on recent advances in cognitive neuroscience, the author presents a formulation in which the marked persistence of anorexia nervosa can be usefully understood as a well-ingrained maladaptive habit. Method The author reviewed the relevant literature on the development and course of anorexia nervosa and interpreted critical features in light of developments in cognitive neuroscience. Results Anorexia nervosa is a well characterized disorder with remarkable persistence both across history and among affected individuals. Food restriction, the salient behavioral feature of the disorder, often begins innocently but gradually takes on a life of its own. Over time, it becomes highly entrenched and resistant to change through either psychological or pharmacological treatment. Cognitive neuroscience has described two related but distinct processes that underlie the acquisition of new patterns of behavior, namely, action-outcome and stimulus-response learning. It is likely that both processes are engaged in the development of anorexia nervosa and that stimulus-response learning (that is, habit formation) is critical to the persistence of the dieting behavior. Conclusions The formulation of the dieting behavior characteristic of anorexia nervosa as a well-entrenched habit provides a basis for understanding the striking persistence of this disorder. This model helps explain the resistance of anorexia nervosa to interventions that have established efficacy in related disorders and implies that addressing the dieting behavior is critical, especially early in the course of the illness, before it has become ingrained. PMID:23429750

Survival of Pseudomonas aeruginosa exposed to sunlight resembles the phenom of persistence.

PubMed

Forte Giacobone, Ana F; Oppezzo, Oscar J

2015-01-01

During exposure of Pseudomonas aeruginosa stationary phase cells to natural solar radiation, a reduction in the rate of loss of bacterial viability was observed when survival fractions were lower than 1/10,000. This reduction was independent of the growth medium used and of the initial bacterial concentration, and was also observed when irradiation was performed with artificial UVA radiation (365nm, 47Wm(-2)). These results indicate the presence of a small bacterial subpopulation with increased tolerance to radiation. Such a tolerance is non-heritable, since survival curves comparable to those of the parental strain were obtained from survivors to long-term exposure to radiation. The radiation response described here resembles the phenomenon called persistence, which consists of the presence of a small subpopulation of slow-growing cells which are able to survive antibiotic treatment within a susceptible bacterial population. The condition of persister cells is acquired via a reversible switch and involves active defense systems towards oxidative stress. Persistence is probably responsible for biphasic responses of bacteria to several stress conditions, one of which may be exposure to sunlight. The models currently used to analyze the lethal action of sunlight overestimate the effect of high-dose irradiation. These models could be improved by including the potential formation of persister cells. Copyright © 2014 Elsevier B.V. All rights reserved.

Antimicrobial resistance, biofilm formation and virulence reveal Actinobacillus pleuropneumoniae strains' pathogenicity complexity.

PubMed

Pereira, Monalessa Fábia; Rossi, Ciro César; Seide, Larissa Eler; Martins Filho, Sebastião; Dolinski, Cláudia de Melo; Bazzolli, Denise Mara Soares

2018-05-07

Porcine pleuropneumonia is an important cause of lowered productivity and economic loss in the pig industry worldwide, associated primarily with Actinobacillus pleuropneumoniae infection. Its colonization and persistence within the upper respiratory tract of affected pigs depends upon interactions between a number of genetically controlled virulence factors, such as pore-forming repeats-in-toxin exoproteins, biofilm formation, and antimicrobial resistance. This study investigated correlations between biofilm-forming capacity, antimicrobial resistance, and virulence of A. pleuropneumoniae obtained from clinical outbreaks of disease, using a Galleria mellonella alternative infection model. Results suggest that virulence is diverse amongst the 21 strains of A. pleuropneumoniae examined and biofilm formation correlated with genetic control of antimicrobial resistance. Copyright © 2018 Elsevier Ltd. All rights reserved.

Biofilm Formation and Disinfectant Susceptibility of Persistent and Nonpersistent Listeria monocytogenes Isolates from Gorgonzola Cheese Processing Plants.

PubMed

Costa, Annalisa; Bertolotti, Luigi; Brito, Luisa; Civera, Tiziana

2016-11-01

The aim of this study was to investigate whether the biofilm-forming ability and/or the disinfectant susceptibility accounted for the persistence of Listeria monocytogenes in Gorgonzola cheese processing plants. For this purpose, a set of 16 L. monocytogenes isolates collected in the 2004-2007 period was analyzed, including 11 persistent isolates collected in different years, within the collection period, and displaying identical or highly correlated pulsotypes. The evaluation of biofilm-forming ability was assessed using crystal violet (CV) staining and the enumeration of viable cells on stainless steel coupons (SSC). Absorbance values obtained with CV staining for persistent and nonpersistent isolates were not significantly different (rm-ANOVA p > 0.05) and the cell counts from nonpersistent isolates showed to be higher compared with persistent isolates (rm-ANOVA p < 0.05). A simulation of disinfectant treatments was performed on spot inoculated coupons in clean and dirty conditions, according to EN 13697, and on biofilms on SSC, grown in nutrient-rich (dirty) and limiting (clean) conditions using acid acetic-hydrogen peroxide (P3) and acid citric-hydrogen peroxide (MS) commercial disinfectants. The treatment was considered effective when a 4 Log reduction in viable cell count was observed. The Log reductions of persistent and nonpersistent isolates, obtained with both the assays in clean and dirty conditions, were compared and no significant differences were detected (rm-ANOVA p > 0.05). A greater influence of organic matter on MS could explain why P3 was efficient in reducing to effective levels the majority of the isolates at the lowest concentration suggested by the manufacturer (0.2% [v/v]), while the same purpose required a higher concentration (1% [v/v]) of MS. In conclusion, our results demonstrate that the persistence of these isolates in Gorgonzola cheese processing plants was linked neither to the biofilm-forming ability nor to their susceptibility to hydrogen peroxide-based disinfectants; therefore, other factors should contribute to the persistent colonization of the dairies.

A cell-based assay to assess the persistence of action of agonists acting at recombinant human beta(2) adrenoceptors.

PubMed

Summerhill, Susan; Stroud, Timothy; Nagendra, Roshini; Perros-Huguet, Christelle; Trevethick, Michael

2008-01-01

The aim was to establish a robust, 96-well, cell-based assay to assess the potency and persistence of action of agonists acting at human recombinant beta(2) adrenoceptors expressed in CHO (Chinese Hamster Ovary) cells and to compare this with published duration of action data in guinea pig isolated trachea and human bronchus. Cells were treated with either: (i) beta-adrenoceptor agonist for 30 min, washed and cyclicAMP (cAMP) measured 30 min later-termed 'washed' cells or, (ii) treated with solvent for 30 min, washed, and then treated with beta-adrenoceptor agonist for 30 min and cAMP measured-termed 'unwashed' cells. The 'washed' EC(50) was divided by the 'unwashed' EC(50) to determine a rightward shift concentration ratio, which was indicative of the persistence of action at the receptor. At the beta(2) adrenoceptor salmeterol, carmoterol and indacaterol were resistant to washing with a concentration ratio of <5, indicating a long persistence of action, whereas formoterol, isoprenaline and salbutamol were washed out with a ratio of 32, >294 and >800 respectively, suggesting a shorter persistence of action. At beta(1) and beta(3) adrenoceptors all compounds washed out. The persistent effects of salmeterol at beta(2) following washing could be reversed by the selective beta(2) antagonist ICI 118551, suggesting continued receptor activation. The data presented agree well with published data assessing duration of action of beta(2) agonists in human isolated bronchus and guinea pig isolated trachea. Key features are: (a) it is a 96-well format which can be used to assess many compounds in a single experiment, (b) both potency and persistence of agonist action are assessed in the same assay, (c) any effects of concentration on the persistence of action can be highlighted, and (d) it allows triage of compounds prior to tissue bath studies thus reducing the use of animal tissue.

Mib1 contributes to persistent directional cell migration by regulating the Ctnnd1-Rac1 pathway.

PubMed

Mizoguchi, Takamasa; Ikeda, Shoko; Watanabe, Saori; Sugawara, Michiko; Itoh, Motoyuki

2017-10-31

Persistent directional cell migration is involved in animal development and diseases. The small GTPase Rac1 is involved in F-actin and focal adhesion dynamics. Local Rac1 activity is required for persistent directional migration, whereas global, hyperactivated Rac1 enhances random cell migration. Therefore, precise control of Rac1 activity is important for proper directional cell migration. However, the molecular mechanism underlying the regulation of Rac1 activity in persistent directional cell migration is not fully understood. Here, we show that the ubiquitin ligase mind bomb 1 (Mib1) is involved in persistent directional cell migration. We found that knockdown of MIB1 led to an increase in random cell migration in HeLa cells in a wound-closure assay. Furthermore, we explored novel Mib1 substrates for cell migration and found that Mib1 ubiquitinates Ctnnd1. Mib1-mediated ubiquitination of Ctnnd1 K547 attenuated Rac1 activation in cultured cells. In addition, we found that posterior lateral line primordium cells in the zebrafish mib1 ta52b mutant showed increased random migration and loss of directional F-actin-based protrusion formation. Knockdown of Ctnnd1 partially rescued posterior lateral line primordium cell migration defects in the mib1 ta52b mutant. Taken together, our data suggest that Mib1 plays an important role in cell migration and that persistent directional cell migration is regulated, at least in part, by the Mib1-Ctnnd1-Rac1 pathway. Published under the PNAS license.

A study of ingestion and dispersion of engine exhaust products in trailing vortex systems

NASA Technical Reports Server (NTRS)

Nielsen, J. N.; Stahara, S. S.; Woolley, J. P.

1973-01-01

Analysis has been made of the ingestion and dispersion of engine exhaust products into the trailing vortex system of supersonic aircraft flying in the stratosphere. The rate of mixing between the supersonic jet and the co-flowing supersonic stream was found to be an order of magnitude less than would be expected on the basis of subsonic eddy-viscosity results. The length of the potential core was 66 nozzle exit radii so that the exhaust gases remain at elevated temperatures and concentrations over much longer distances than previsously estimated. Ingestion started at the end of the potential core and all hot gas from the engine was ingested into the trailing vortex within two core lengths. Comparison between the buoyancy calculations for the supersonic case with nondimensionalized subsonic aircraft contrail data on wake spreading showed good agreement. Velocity and temperature profiles have been specified at various stages of the wake, and the analysis in this report can be used to predict variations of concentrations of species such as nitrogen oxides under conditions of chemical reaction.

Radiative Forcing Over Ocean by Ship Wakes

NASA Technical Reports Server (NTRS)

Gatebe, Charles K.; Wilcox, E.; Poudyal, R.; Wang, J.

2011-01-01

Changes in surface albedo represent one of the main forcing agents that can counteract, to some extent, the positive forcing from increasing greenhouse gas concentrations. Here, we report on enhanced ocean reflectance from ship wakes over the Pacific Ocean near the California coast, where we determined, based on airborne radiation measurements that ship wakes can increase reflected sunlight by more than 100%. We assessed the importance of this increase to climate forcing, where we estimated the global radiative forcing of ship wakes to be -0.00014 plus or minus 53% Watts per square meter assuming a global distribution of 32331 ships of size of greater than or equal to 100000 gross tonnage. The forcing is smaller than the forcing of aircraft contrails (-0.007 to +0.02 Watts per square meter), but considering that the global shipping fleet has rapidly grown in the last five decades and this trend is likely to continue because of the need of more inter-continental transportation as a result of economic globalization, we argue that the radiative forcing of wakes is expected to be increasingly important especially in harbors and coastal regions.

Systemic reactive angioendotheliomatosis-like syndrome in a steer presumed to be persistently infected with bovine viral diarrhea virus.

PubMed

Breshears, M A; Johnson, B J

2008-09-01

Unusual proliferative intravascular lesions were seen in multiple organs of a 2-year-old Corriente steer presumed to be persistently infected with bovine viral diarrhea virus (BVDV), based on widespread immunohistochemical detection of BVDV antigen. Proliferations of spindle cells, which were immunohistochemically positive for von Willebrand factor-related antigen, partially-to-completely occluded vessel lumens and were supported by cells that were immunohistochemically positive for smooth muscle actin. Distribution and character of the intraluminal proliferations are strikingly similar to those described in feline systemic reactive angioendotheliomatosis, a rare entity of unknown cause. The presence of occasional intravascular thrombi suggests that the proliferative vasculopathy was associated with an underlying thrombotic process with immunohistochemical similarities to thrombotic thrombocytopenic purpura of humans. Death of the steer was due to hemorrhage from a castration wound, which may indicate thrombocytopenia or platelet dysfunction. The role of persistent BVDV infection in the formation of the intravascular lesions is unknown.

Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales.

PubMed

Daly, Rebecca A; Borton, Mikayla A; Wilkins, Michael J; Hoyt, David W; Kountz, Duncan J; Wolfe, Richard A; Welch, Susan A; Marcus, Daniel N; Trexler, Ryan V; MacRae, Jean D; Krzycki, Joseph A; Cole, David R; Mouser, Paula J; Wrighton, Kelly C

2016-09-05

Hydraulic fracturing is the industry standard for extracting hydrocarbons from shale formations. Attention has been paid to the economic benefits and environmental impacts of this process, yet the biogeochemical changes induced in the deep subsurface are poorly understood. Recent single-gene investigations revealed that halotolerant microbial communities were enriched after hydraulic fracturing. Here, the reconstruction of 31 unique genomes coupled to metabolite data from the Marcellus and Utica shales revealed that many of the persisting organisms play roles in methylamine cycling, ultimately supporting methanogenesis in the deep biosphere. Fermentation of injected chemical additives also sustains long-term microbial persistence, while thiosulfate reduction could produce sulfide, contributing to reservoir souring and infrastructure corrosion. Extensive links between viruses and microbial hosts demonstrate active viral predation, which may contribute to the release of labile cellular constituents into the extracellular environment. Our analyses show that hydraulic fracturing provides the organismal and chemical inputs for colonization and persistence in the deep terrestrial subsurface.

The roles of biofilm matrix polysaccharide Psl in mucoid Pseudomonas aeruginosa biofilms.

PubMed

Ma, Luyan; Wang, Shiwei; Wang, Di; Parsek, Matthew R; Wozniak, Daniel J

2012-07-01

The opportunistic pathogen Pseudomonas aeruginosa causes life-threatening, persistent infections in patients with cystic fibrosis (CF). Persistence is attributed to the ability of these bacteria to form structured communities (biofilms). Biofilms rely on an extracellular polymeric substances matrix to maintain structure. Psl exopolysaccharide is a key matrix component of nonmucoid biofilms, yet the role of Psl in mucoid biofilms is unknown. In this report, using a variety of mutants in a mucoid P. aeruginosa background, we found that deletion of Psl-encoding genes dramatically decreased their biofilm formation ability, indicating that Psl is also a critical matrix component of mucoid biofilms. Our data also suggest that the overproduction of alginate leads to mucoid biofilms, which occupy more space, whereas Psl-dependent biofilms are densely packed. These data suggest that Psl polysaccharide may have significant contributions in biofilm persistence in patients with CF and may be helpful for designing therapies for P. aeruginosa CF infection. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Integrated combined effects of temperature, pH and sodium chloride concentration on biofilm formation by Salmonella enterica ser. Enteritidis and Typhimurium under low nutrient food-related conditions.

PubMed

Iliadis, Ioannis; Daskalopoulou, Aikaterini; Simões, Manuel; Giaouris, Efstathios

2018-05-01

Salmonella enterica is a major foodborne bacterial pathogen. This forms biofilms on surfaces and persists, depending on the strain and the environment. The integrative interaction of temperature (T; 13-39 °C), pH (5-8) and sodium chloride (NaCl) concentration (0.5-8.5%) on biofilm formation by two S. enterica strains (ser. Enteritidis and Typhimurium) was here evaluated under low nutrient conditions. This was achieved using response surface methodology to model the combined effect of each factor on the response, through mathematical quadratic fitting of the outcomes of a sequence of designed experiments. These last were executed by incubating stainless steel coupons carrying sessile bacteria, for 24 h, in 1:10 diluted tryptone soya broth, under 15 different combinations of three independent factors (T, pH and NaCl). For each strain, a second order polynomial model, describing the relationship between biofilm formation (log CFU/cm 2 ) and the factors (T, pH and NaCl), was developed using least square regression analysis. Both derived models predicted the combined influences of these factors on biofilm formation, with agreement between predictions and experimental observations (R 2  ≥ 0.96, P ≤ 0.0001). For both strains, the increase of NaCl content restricted their sessile growth, while under low salinity conditions (NaCl < 4%) biofilm formation was favored as pH increased, regardless of T. Interestingly, under low salt content, and depending on the strain, biofilm formation was either favored or hindered by increasing T. Thus, 34.5 and 13 °C were the T predicted to maximize biofilm formation by strains Enteritidis and Typhimurium, respectively, something which was also experimentally verified. To sum, these models can predict the interactive influences of crucial food-related factors on biofilm growth of a significant foodborne pathogen towards the efforts to limit its persistence in food industry. Copyright © 2018 Elsevier Ltd. All rights reserved.

Long-term memory consolidation: The role of RNA-binding proteins with prion-like domains.

PubMed

Sudhakaran, Indulekha P; Ramaswami, Mani

2017-05-04

Long-term and short-term memories differ primarily in the duration of their retention. At a molecular level, long-term memory (LTM) is distinguished from short-term memory (STM) by its requirement for new gene expression. In addition to transcription (nuclear gene expression) the translation of stored mRNAs is necessary for LTM formation. The mechanisms and functions for temporal and spatial regulation of mRNAs required for LTM is a major contemporary problem, of interest from molecular, cell biological, neurobiological and clinical perspectives. This review discusses primary evidence in support for translational regulatory events involved in LTM and a model in which different phases of translation underlie distinct phases of consolidation of memories. However, it focuses largely on mechanisms of memory persistence and the role of prion-like domains in this defining aspect of long-term memory. We consider primary evidence for the concept that Cytoplasmic Polyadenylation Element Binding (CPEB) protein enables the persistence of formed memories by transforming in prion-like manner from a soluble monomeric state to a self-perpetuating and persistent polymeric translationally active state required for maintaining persistent synaptic plasticity. We further discuss prion-like domains prevalent on several other RNA-binding proteins involved in neuronal translational control underlying LTM. Growing evidence indicates that such RNA regulatory proteins are components of mRNP (RiboNucleoProtein) granules. In these proteins, prion-like domains, being intrinsically disordered, could mediate weak transient interactions that allow the assembly of RNP granules, a source of silenced mRNAs whose translation is necessary for LTM. We consider the structural bases for RNA granules formation as well as functions of disordered domains and discuss how these complicate the interpretation of existing experimental data relevant to general mechanisms by which prion-domain containing RBPs function in synapse specific plasticity underlying LTM.

The Culture of Science and the Rhetoric of Scientism: From Francis Bacon to the Darwin Fish

ERIC Educational Resources Information Center

Lessl, Thomas M.

2007-01-01

The culture of modern science continues to establish its public identity by appealing to values and historical conceptions that reflect its appropriation of various religious ideals during its formative period, most especially in the rhetoric of Francis Bacon. These elements have persisted because they continue to achieve similar goals, but the…

Testing the Limits of Politeness: Youth Group Talk in a Community Organization.

ERIC Educational Resources Information Center

Pazey, Barbara

This paper builds on previous work which analyzed ways of speaking, the use of space, and relationship formation in the work and play of organizations, in order to determine how youth develop a sense of self, empowerment, and persistence. Examined here are the speech interactions taken from three teaching lessons involving youth and youth leaders…

Ontogeny of Contextual Fear Memory Formation, Specificity, and Persistence in Mice

ERIC Educational Resources Information Center

Akers, Katherine G.; Arruda-Carvalho, Maithe; Josselyn, Sheena A.; Frankland, Paul W.

2012-01-01

Pinpointing the precise age when young animals begin to form memories of aversive events is valuable for understanding the onset of anxiety and mood disorders and for detecting early cognitive impairment in models of childhood-onset disorders. Although these disorders are most commonly modeled in mice, we know little regarding the development of…

Depth in an Age of Digital Distraction: The Value of a Catholic College in Today's World

ERIC Educational Resources Information Center

Kelly, Conor M.

2015-01-01

A commitment to holistic student formation in the liberal arts tradition and to the Catholic faith is a hallmark of most Catholic higher education institutions. To be most effective, Catholic institutions must adapt this central mission to changing circumstances in an age of ubiquitous mobile technologies and persistent digital distractions. By…

An Empirical Investigation of Methods for Assessing Item Fit for Mixed Format Tests

ERIC Educational Resources Information Center

Chon, Kyong Hee; Lee, Won-Chan; Ansley, Timothy N.

2013-01-01

Empirical information regarding performance of model-fit procedures has been a persistent need in measurement practice. Statistical procedures for evaluating item fit were applied to real test examples that consist of both dichotomously and polytomously scored items. The item fit statistics used in this study included the PARSCALE's G[squared],…

Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells

PubMed Central

Wang, Jennifer T; Kong, Dong; Hoerner, Christian R; Loncarek, Jadranka

2017-01-01

Centrioles are composed of long-lived microtubules arranged in nine triplets. However, the contribution of triplet microtubules to mammalian centriole formation and stability is unknown. Little is known of the mechanism of triplet microtubule formation, but experiments in unicellular eukaryotes indicate that delta-tubulin and epsilon-tubulin, two less-studied tubulin family members, are required. Here, we report that centrioles in delta-tubulin and epsilon-tubulin null mutant human cells lack triplet microtubules and fail to undergo centriole maturation. These aberrant centrioles are formed de novo each cell cycle, but are unstable and do not persist to the next cell cycle, leading to a futile cycle of centriole formation and disintegration. Disintegration can be suppressed by paclitaxel treatment. Delta-tubulin and epsilon-tubulin physically interact, indicating that these tubulins act together to maintain triplet microtubules and that these are necessary for inheritance of centrioles from one cell cycle to the next. PMID:28906251

Timing of the formation and migration of giant planets as constrained by CB chondrites

PubMed Central

Johnson, Brandon C.; Walsh, Kevin J.; Minton, David A.; Krot, Alexander N.; Levison, Harold F.

2016-01-01

The presence, formation, and migration of giant planets fundamentally shape planetary systems. However, the timing of the formation and migration of giant planets in our solar system remains largely unconstrained. Simulating planetary accretion, we find that giant planet migration produces a relatively short-lived spike in impact velocities lasting ~0.5 My. These high-impact velocities are required to vaporize a significant fraction of Fe,Ni metal and silicates and produce the CB (Bencubbin-like) metal-rich carbonaceous chondrites, a unique class of meteorites that were created in an impact vapor-melt plume ~5 My after the first solar system solids. This indicates that the region where the CB chondrites formed was dynamically excited at this early time by the direct interference of the giant planets. Furthermore, this suggests that the formation of the giant planet cores was protracted and the solar nebula persisted until ~5 My. PMID:27957541

Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells.

PubMed

Wang, Jennifer T; Kong, Dong; Hoerner, Christian R; Loncarek, Jadranka; Stearns, Tim

2017-09-14

Centrioles are composed of long-lived microtubules arranged in nine triplets. However, the contribution of triplet microtubules to mammalian centriole formation and stability is unknown. Little is known of the mechanism of triplet microtubule formation, but experiments in unicellular eukaryotes indicate that delta-tubulin and epsilon-tubulin, two less-studied tubulin family members, are required. Here, we report that centrioles in delta-tubulin and epsilon-tubulin null mutant human cells lack triplet microtubules and fail to undergo centriole maturation. These aberrant centrioles are formed de novo each cell cycle, but are unstable and do not persist to the next cell cycle, leading to a futile cycle of centriole formation and disintegration. Disintegration can be suppressed by paclitaxel treatment. Delta-tubulin and epsilon-tubulin physically interact, indicating that these tubulins act together to maintain triplet microtubules and that these are necessary for inheritance of centrioles from one cell cycle to the next.

Timing of the formation and migration of giant planets as constrained by CB chondrites.

PubMed

Johnson, Brandon C; Walsh, Kevin J; Minton, David A; Krot, Alexander N; Levison, Harold F

2016-12-01

The presence, formation, and migration of giant planets fundamentally shape planetary systems. However, the timing of the formation and migration of giant planets in our solar system remains largely unconstrained. Simulating planetary accretion, we find that giant planet migration produces a relatively short-lived spike in impact velocities lasting ~0.5 My. These high-impact velocities are required to vaporize a significant fraction of Fe,Ni metal and silicates and produce the CB (Bencubbin-like) metal-rich carbonaceous chondrites, a unique class of meteorites that were created in an impact vapor-melt plume ~5 My after the first solar system solids. This indicates that the region where the CB chondrites formed was dynamically excited at this early time by the direct interference of the giant planets. Furthermore, this suggests that the formation of the giant planet cores was protracted and the solar nebula persisted until ~5 My.

Supraglacial Lakes in the Percolation Zone of the Western Greenland Ice Sheet: Formation and Development using Operation IceBridge Snow Radar and ATM (2009-2014)

NASA Astrophysics Data System (ADS)

Chen, C.; Howat, I. M.; de la Peña, S.

2015-12-01

Surface meltwater lakes on the Greenland Ice Sheet have appeared at higher elevations, extending well into the percolation zone, under recent warming, with the largest expansion occurring in the western Greenland Ice Sheet. The conditions that allow lakes to form atop firn are poorly constrained, but the formation of new lakes imply changes in the permeability of the firn at high elevations, promoting meltwater runoff. We explore the formation and evolution of new surface lakes in this region above 1500 meters, using a combination of satellite imagery and repeat Snow (2-6.5 GHz) radar echograms and LIDAR measurements from NASA's Operation IceBridge of 2009-2014. We identify conditions for surface lake formation at their farthest inland extent and suggest behaviors of persistence and lake drainage are due to differences in regional ice dynamics.

Extracellular DNA facilitates the formation of functional amyloids in Staphylococcus aureus biofilms.

PubMed

Schwartz, Kelly; Ganesan, Mahesh; Payne, David E; Solomon, Michael J; Boles, Blaise R

2016-01-01

Persistent staphylococcal infections often involve surface-associated communities called biofilms. Staphylococcus aureus biofilm development is mediated by the co-ordinated production of the biofilm matrix, which can be composed of polysaccharides, extracellular DNA (eDNA) and proteins including amyloid fibers. The nature of the interactions between matrix components, and how these interactions contribute to the formation of matrix, remain unclear. Here we show that the presence of eDNA in S. aureus biofilms promotes the formation of amyloid fibers. Conditions or mutants that do not generate eDNA result in lack of amyloids during biofilm growth despite the amyloidogeneic subunits, phenol soluble modulin peptides, being produced. In vitro studies revealed that the presence of DNA promotes amyloid formation by PSM peptides. Thus, this work exposes a previously unacknowledged interaction between biofilm matrix components that furthers our understanding of functional amyloid formation and S. aureus biofilm biology. © 2015 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

Molecular dynamics simulations of polyelectrolyte brushes under poor solvent conditions: origins of bundle formation.

PubMed

He, Gui-Li; Merlitz, Holger; Sommer, Jens-Uwe

2014-03-14

Molecular dynamics simulations are applied to investigate salt-free planar polyelectrolyte brushes under poor solvent conditions. Starting above the Θ-point with a homogeneous brush and then gradually reducing the temperature, the polymers initially display a lateral structure formation, forming vertical bundles of chains. A further reduction of the temperature (or solvent quality) leads to a vertical collapse of the brush. By varying the size and selectivity of the counterions, we show that lateral structure formation persists and therefore demonstrate that the entropy of counterions being the dominant factor for the formation of the bundle phase. By applying an external compression force on the brush we calculate the minimal work done on the polymer phase only and prove that the entropy gain of counterions in the bundle state, as compared to the homogeneously collapsed state at the same temperature, is responsible for the lateral microphase segregation. As a consequence, the observed lateral structure formation has to be regarded universal for osmotic polymer brushes below the Θ-point.

Hole punch clouds over the Bahamas

NASA Image and Video Library

2017-12-08

In elementary school, students learn that water freezes at 0 degrees Celsius (32 degrees Fahrenheit). That is true most of the time, but there are exceptions to the rule. For instance, water with very few impurities (such as dust or pollution particles, fungal spores, bacteria) can be chilled to much cooler temperatures and still remain liquid—a process known as supercooling. Supercooling may sound exotic, but it occurs pretty routinely in Earth’s atmosphere. Altocumulus clouds, a common type of mid-altitude cloud, are mostly composed of water droplets supercooled to a temperature of about -15 degrees C. Altocumulus clouds with supercooled tops cover about 8 percent of Earth’s surface at any given time. Supercooled water droplets play a key role in the formation of hole-punch and canal clouds, the distinctive clouds shown in these satellite images. Hole-punch clouds usually appear as circular gaps in decks of altocumulus clouds; canal clouds look similar but the gaps are longer and thinner. This true-color image shows hole-punch and canal clouds off the coast of Florida, as observed on December 12, 2014, by the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA’s Terra satellite. Both types of cloud form when aircraft fly through cloud decks rich with supercooled water droplets and produce aerodynamic contrails. Air expands and cools as it moves around the wings and past the propeller, a process known as adiabatic cooling. Air temperatures over jet wings often cool by as much as 20 degrees Celsius, pushing supercooled water droplets to the point of freezing. As ice crystals form, they absorb nearby water droplets. Since ice crystals are relatively heavy, they tend to sink. This triggers tiny bursts of snow or rain that leave gaps in the cloud cover. Whether a cloud formation becomes a hole-punch or canal depends on the thickness of the cloud layer, the air temperature, and the degree of horizontal wind shear. Both descending and ascending aircraft—including jets and propeller planes—can trigger hole-punch and canal clouds. The nearest major airports in the images above include Miami International, Fort Lauderdale International, Grand Bahama International, and Palm Beach International. Credit: NASA/GSFC/Jeff Schmaltz/MODIS Land Rapid Response Team NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Severe Acute Local Reactions to a Hyaluronic Acid-derived Dermal Filler

PubMed Central

Hays, Geoffrey P.; Caglia, Anthony E.; Caglia, Michael

2010-01-01

Injectable fillers are normally well tolerated by patients with little or no adverse effects. The most common side effects include swelling, redness, bruising, and pain at the injection site. This report describes three cases in which patients injected with a hyaluronic acid-derived injectable filler that is premixed with lidocaine developed adverse reactions including persistent swelling, pain, and nodule formation. Two of the three patients' abscesses were cultured for aerobic and anaerobic bacteria and mycobacterium. All three cultures were negative. Abscess persistence in all cases necessitated physical removal and/or enzymatic degradation with hyaluronidase. The effects subsided only after the product had been removed. Two of these patients were subsequently treated with other hyaluronic acid-derived dermal fillers without adverse events. PMID:20725567

Transcriptome Sequencing Reveals Wide Expression Reprogramming of Basal and Unknown Genes in Leptospira biflexa Biofilms

PubMed Central

Spangenberg, Lucía; Lopes Bastos, Bruno; Graña, Martín; Vasconcelos, Larissa; Almeida, Áurea; Greif, Gonzalo; Robello, Carlos; Ristow, Paula

2016-01-01

ABSTRACT The genus Leptospira is composed of pathogenic and saprophytic spirochetes. Pathogenic Leptospira is the etiological agent of leptospirosis, a globally spread neglected disease. A key ecological feature of some pathogenic species is their ability to survive both within and outside the host. For most leptospires, the ability to persist outside the host is associated with biofilm formation, a most important bacterial strategy to face and overcome hostile environmental conditions. The architecture and biochemistry of leptospiral biofilms are rather well understood; however, the genetic program underpinning biofilm formation remains mostly unknown. In this work, we used the saprophyte Leptospira biflexa as a model organism to assess over- and underrepresented transcripts during the biofilm state, using transcriptome sequencing (RNA-seq) technology. Our results showed that some basal biological processes like DNA replication and cell division are downregulated in the mature biofilm. Additionally, we identified significant expression reprogramming for genes involved in motility, sugar/lipid metabolism, and iron scavenging, as well as for outer membrane-encoding genes. A careful manual annotation process allowed us to assign molecular functions to many previously uncharacterized genes that are probably involved in biofilm metabolism. We also provided evidence for the presence of small regulatory RNAs in this species. Finally, coexpression networks were reconstructed to pinpoint functionally related gene clusters that may explain how biofilm maintenance is regulated. Beyond elucidating some genetic aspects of biofilm formation, this work reveals a number of pathways whose functional dissection may impact our understanding of leptospiral biology, in particular how these organisms adapt to environmental changes. IMPORTANCE In this work, we describe the first transcriptome based on RNA-seq technology focused on studying transcriptional changes associated with biofilm growth in a member of the genus Leptospira. As many pathogenic species of this genus can survive inside the host but also persist in environmental water, mostly forming biofilms, identifying the molecular basis of this capacity can impact the understanding of how leptospires are able to fulfill a complete life cycle that alternates between adaptation to the host and adaptation to hostile external environmental conditions. We identified several genes and regulatory networks that can be the kickoff for deepening understanding of the molecular mechanisms involving bacterial persistence via biofilm formation; understanding this is important for the future development of tools for controlling leptospirosis. PMID:27303713

Transcriptome Sequencing Reveals Wide Expression Reprogramming of Basal and Unknown Genes in Leptospira biflexa Biofilms.

PubMed

Iraola, Gregorio; Spangenberg, Lucía; Lopes Bastos, Bruno; Graña, Martín; Vasconcelos, Larissa; Almeida, Áurea; Greif, Gonzalo; Robello, Carlos; Ristow, Paula; Naya, Hugo

2016-01-01

The genus Leptospira is composed of pathogenic and saprophytic spirochetes. Pathogenic Leptospira is the etiological agent of leptospirosis, a globally spread neglected disease. A key ecological feature of some pathogenic species is their ability to survive both within and outside the host. For most leptospires, the ability to persist outside the host is associated with biofilm formation, a most important bacterial strategy to face and overcome hostile environmental conditions. The architecture and biochemistry of leptospiral biofilms are rather well understood; however, the genetic program underpinning biofilm formation remains mostly unknown. In this work, we used the saprophyte Leptospira biflexa as a model organism to assess over- and underrepresented transcripts during the biofilm state, using transcriptome sequencing (RNA-seq) technology. Our results showed that some basal biological processes like DNA replication and cell division are downregulated in the mature biofilm. Additionally, we identified significant expression reprogramming for genes involved in motility, sugar/lipid metabolism, and iron scavenging, as well as for outer membrane-encoding genes. A careful manual annotation process allowed us to assign molecular functions to many previously uncharacterized genes that are probably involved in biofilm metabolism. We also provided evidence for the presence of small regulatory RNAs in this species. Finally, coexpression networks were reconstructed to pinpoint functionally related gene clusters that may explain how biofilm maintenance is regulated. Beyond elucidating some genetic aspects of biofilm formation, this work reveals a number of pathways whose functional dissection may impact our understanding of leptospiral biology, in particular how these organisms adapt to environmental changes. IMPORTANCE In this work, we describe the first transcriptome based on RNA-seq technology focused on studying transcriptional changes associated with biofilm growth in a member of the genus Leptospira. As many pathogenic species of this genus can survive inside the host but also persist in environmental water, mostly forming biofilms, identifying the molecular basis of this capacity can impact the understanding of how leptospires are able to fulfill a complete life cycle that alternates between adaptation to the host and adaptation to hostile external environmental conditions. We identified several genes and regulatory networks that can be the kickoff for deepening understanding of the molecular mechanisms involving bacterial persistence via biofilm formation; understanding this is important for the future development of tools for controlling leptospirosis.

Spatiotemporal characterization of ionizing radiation induced DNA damage foci and their relation to chromatin organization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Costes, Sylvain V; Chiolo, Irene; Pluth, Janice M.

2009-09-15

DNA damage sensing proteins have been shown to localize to the sites of DSB within seconds to minutes following ionizing radiation (IR) exposure, resulting in the formation of microscopically visible nuclear domains referred to as radiation-induced foci (RIF). This review characterizes the spatio-temporal properties of RIF at physiological doses, minutes to hours following exposure to ionizing radiation, and it proposes a model describing RIF formation and resolution as a function of radiation quality and nuclear densities. Discussion is limited to RIF formed by three interrelated proteins ATM (Ataxia telangiectasia mutated), 53BP1 (p53 binding protein 1) and ?H2AX (phosphorylated variant histonemore » H2AX). Early post-IR, we propose that RIF mark chromatin reorganization, leading to a local nuclear scaffold rigid enough to keep broken DNA from diffusing away, but open enough to allow the repair machinery. We review data indicating clear kinetic and physical differences between RIF emerging from dense and uncondensed regions of the nucleus. At later time post-IR, we propose that persistent RIF observed days following exposure to ionizing radiation are nuclear ?scars? marking permanent disruption of the chromatin architecture. When DNA damage is resolved, such chromatin modifications should not necessarily lead to growth arrest and it has been shown that persistent RIF can replicate during mitosis. Thus, heritable persistent RIF spanning over tens of Mbp may affect the transcriptome of a large progeny of cells. This opens the door for a non DNA mutation-based mechanism of radiation-induced phenotypes.« less

A branching process model for the analysis of abortive colony size distributions in carbon ion-irradiated normal human fibroblasts.

PubMed

Sakashita, Tetsuya; Hamada, Nobuyuki; Kawaguchi, Isao; Hara, Takamitsu; Kobayashi, Yasuhiko; Saito, Kimiaki

2014-05-01

A single cell can form a colony, and ionizing irradiation has long been known to reduce such a cellular clonogenic potential. Analysis of abortive colonies unable to continue to grow should provide important information on the reproductive cell death (RCD) following irradiation. Our previous analysis with a branching process model showed that the RCD in normal human fibroblasts can persist over 16 generations following irradiation with low linear energy transfer (LET) γ-rays. Here we further set out to evaluate the RCD persistency in abortive colonies arising from normal human fibroblasts exposed to high-LET carbon ions (18.3 MeV/u, 108 keV/µm). We found that the abortive colony size distribution determined by biological experiments follows a linear relationship on the log-log plot, and that the Monte Carlo simulation using the RCD probability estimated from such a linear relationship well simulates the experimentally determined surviving fraction and the relative biological effectiveness (RBE). We identified the short-term phase and long-term phase for the persistent RCD following carbon-ion irradiation, which were similar to those previously identified following γ-irradiation. Taken together, our results suggest that subsequent secondary or tertiary colony formation would be invaluable for understanding the long-lasting RCD. All together, our framework for analysis with a branching process model and a colony formation assay is applicable to determination of cellular responses to low- and high-LET radiation, and suggests that the long-lasting RCD is a pivotal determinant of the surviving fraction and the RBE.

Stability and nonlinear adjustment of vortices in Keplerian flows

NASA Astrophysics Data System (ADS)

Bodo, G.; Tevzadze, A.; Chagelishvili, G.; Mignone, A.; Rossi, P.; Ferrari, A.

2007-11-01

Aims:We investigate the stability, nonlinear development and equilibrium structure of vortices in a background shearing Keplerian flow Methods: We make use of high-resolution global two-dimensional compressible hydrodynamic simulations. We introduce the concept of nonlinear adjustment to describe the transition of unbalanced vortical fields to a long-lived configuration. Results: We discuss the conditions under which vortical perturbations evolve into long-lived persistent structures and we describe the properties of these equilibrium vortices. The properties of equilibrium vortices appear to be independent from the initial conditions and depend only on the local disk parameters. In particular we find that the ratio of the vortex size to the local disk scale height increases with the decrease of the sound speed, reaching values well above the unity. The process of spiral density wave generation by the vortex, discussed in our previous work, appear to maintain its efficiency also at nonlinear amplitudes and we observe the formation of spiral shocks attached to the vortex. The shocks may have important consequences on the long term vortex evolution and possibly on the global disk dynamics. Conclusions: Our study strengthens the arguments in favor of anticyclonic vortices as the candidates for the promotion of planetary formation. Hydrodynamic shocks that are an intrinsic property of persistent vortices in compressible Keplerian flows are an important contributor to the overall balance. These shocks support vortices against viscous dissipation by generating local potential vorticity and should be responsible for the eventual fate of the persistent anticyclonic vortices. Numerical codes have be able to resolve shock waves to describe the vortex dynamics correctly.

Ecological persistence in the Late Mississippian (Serpukhovian, Namurian A) Megafloral record of the Upper Silesian Basin, Czech Republic

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gastaldo, R.A.; Purkynova, E.; Simunek, Z.

2009-05-15

The Serpukhovian (Namurian A) stratigraphy of the Ostrava Formation, Upper Silesian Coal Basin, Czech Republic, consists of coal-bearing paralic sediments underlain by marine deposits in a cyclothemic nature similar to those in the Pennsylvanian of Euramerica. The thickness of the formation exceeds 3000 m, in which >170 coals are identified in a foreland basin setting. Fifty-five genetic cycles are identified in the present study, using transgressional erosional surfaces as lower and upper boundaries. Terrestrial plant-macrofossil assemblages are preserved within each cycle, mostly associated with coals, and these represent a sampling of the coastal plain vegetation. New high-precision isotope dilution-thermal ionizationmore » mass spectrometry U-Pb ages on zircons from tonsteins of two coals provide chronometric constraints for the Serpukhovian. Unweighted Pair Group Method with Arithmetic Mean clustering and Bayesian statistical classification group macrofloral assemblages into four distinct stratigraphic clusters, with assemblages persisting for <18 cycles before compositional change. Cycle duration, based on Ludmila (328.84{+-}0.16 Ma) and Karel (328.01{+-}0.08 Ma) tonsteins, overlaps the short-period (100 kyr) eccentricity cycle at the 95% confidence interval. These dates push the beginning of the Serpukhovian several million years deeper in time. An estimate for the Visean-Serpukhovian boundary is proposed at similar to 330 Ma. Late Mississippian wetland ecosystems persisted for >1.8 million years before regional perturbation, extirpation, or extinction of taxa occurred. Significant changes in the composition of macrofloral clusters occur across major marine intervals.« less

Far-reaching transport of Pearl River plume water by upwelling jet in the northeastern South China Sea

NASA Astrophysics Data System (ADS)

Chen, Zhaoyun; Pan, Jiayi; Jiang, Yuwu; Lin, Hui

2017-09-01

Satellite images from the Moderate Resolution Imaging Spectroradiometer (MODIS) show that there was a belt of turbid water appearing along an upwelling front near the Chinese coast of Guangdong, and indicate that the turbid water of the Pearl River plume water could be transported to a far-reaching area east of the Taiwan Bank. Numerical modeling results are consistent with the satellite observations, and reveal that a strong jet exists at the upwelling front with a speed as high as 0.8 m s- 1, which acts as a pathway for transporting the high-turbidity plume water. The dynamical analysis suggests that geostrophic equilibrium dominates in the upwelling front and plume areas, and the baroclinicity of the upwelling front resulting from the horizontal density gradient is responsible for the generation of the strong jet, which enhances the far-reaching transport of the terrigenous nutrient-rich water of the Pearl River plume. Model sensitivity analyses also confirm that this jet persists as long as the upwelling front exists, even when the wind subsides and becomes insignificant. Further idealized numerical model experiments indicate that the formation and persistence of the upwelling front jet depend on the forcing strength of the upwelling-favorable wind. The formation time of the jet varies from 15 to 158 h as the stress of the upwelling-favorable wind changes from 0.2 to 0.01 N m- 2. With the persistent transport of the nutrient-rich plume water, biophysical activities can be promoted significantly in the far-reaching destination area of the oligotrophic water.

Activation of ERK2 in basolateral amygdala underlies the promoting influence of stress on fear memory and anxiety: influence of midazolam pretreatment.

PubMed

Maldonado, N M; Espejo, P J; Martijena, I D; Molina, V A

2014-02-01

Exposure to emotionally arousing experiences elicits a robust and persistent memory and enhances anxiety. The amygdala complex plays a key role in stress-induced emotional processing and in the fear memory formation. It is well known that ERK activation in the amygdala is a prerequisite for fear memory consolidation. Moreover, stress elevates p-ERK2 levels in several areas of the brain stress circuitry. Therefore, given that the ERK1/2 cascade is activated following stress and that the role of this cascade is critical in the formation of fear memory, the present study investigated the potential involvement of p-ERK2 in amygdala subnuclei in the promoting influence of stress on fear memory formation and on anxiety-like behavior. A robust and persistent ERK2 activation was noted in the Basolateral amygdala (BLA), which was evident at 5min after restraint and lasted at least one day after the stressful experience. Midazolam, a short-acting benzodiazepine ligand, administered prior to stress prevented the increase in the p-ERK2 level in the BLA. Pretreatment with intra-BLA infusion of U0126 (MEK inhibitor), but not into the adjacent central nucleus of the amygdala, attenuated the stress-induced promoting influence on fear memory formation. Finally, U0126 intra-BLA infusion prevented the enhancement of anxiety-like behavior in stressed animals. These findings suggest that the selective ERK2 activation in BLA following stress exposure is an important mechanism for the occurrence of the promoting influence of stress on fear memory and on anxiety-like behavior. © 2013 Published by Elsevier B.V. and ECNP.

[Correlation of Persistent Free Radicals, PCDD/Fs and Metals in Waste Incineration Fly Ash].

PubMed

Wang, Tian-jiao; Chen, Tong; Zhan, Ming-xiu; Guo, Ying; Li, Xiao-dong

2016-03-15

Environmentally persistent free radicals (EPFRs) are relatively highly stable and found in the formation of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs). Recent studies have concentrated on model dioxin formation reactions and there are few studies on actual waste incineration fly ash. In order to study EPFRs and the correlation with dioxins and heavy metals in waste incineration fly ash, the spins of EPFRs, concentration of PCDD/Fs and metals in samples from 6 different waste incinerators were detected. The medical waste incineration fly ash from Tianjin, municipal solid waste incineration fly ash from Jiangxi Province, black carbon and slag from municipal solid waste incinerator in Lanxi, Zhejiang Province, all contained EPFRs. Above all the signal in Tianjin sample was the strongest. Hydroxyl radicals, carbon-center radicals and semiquinone radicals were detected. Compared with other samples, Jiangxi fly ash had the highest toxic equivalent quantity (TEQ) of dioxins, up to 7.229 4 ng · g⁻¹. However, the dioxin concentration in the Tianjin sample containing the strongest EPFR signals was only 0.092 8 ng · g⁻¹. There was perhaps little direct numeric link between EPFRs and PCDD/Fs. But the spins of EPFRs in samples presented an increasing trend as the metal contents increased, especially with Al, Fe, Zn. The signal strength of radicals was purposed to be related to the metal contents. The concentration of Zn (0.813 7% ) in the Tianjin sample was the highest and this sample contained much more spins of oxygen-center radicals. We could presume the metal Zn had a greater effect on the formation of EPFRs, and was easier to induce the formation of radicals with a longer half-life period.

Bone morphogenetic protein-mediated interaction of periosteum and diaphysis. Citric acid and other factors influencing the generation of parosteal bone.

PubMed

Kübler, N; Urist, M R

1990-09-01

In rabbits, after long-bone growth is complete and the cambium layer regresses, mesenchymal-type cells with embryonic potential (competence) for bone development persist in the adventitial layer of periosteum. These cells are not determined osteoprogenitor cells (stem cells) because bone tissue differentiation does not occur when adult periosteum is transplanted into a heterotopic site. In this respect, adventitial cells differ from bone marrow stroma cells. In a parosteal orthotopic site in the space between the adult periosteum and diaphysis, implants of bone morphogenetic protein (BMP) and associated noncollagenous proteins (BMP/NCP) induce adventitia and adjacent muscle connective-tissue-derived cells to switch from a fibrogenetic to a chondroosteoprogenetic pattern of bone development. The quantity of induced bone is proportional to the dose of BMP/NCP in the range from 10 to 50 mg; immature rabbits produced larger deposits than mature rabbits in response to BMP/NCP. Preoperative local intramuscular injections of citric, edetic, or hyaluronic acids in specified concentrations markedly enhanced subperiosteal BMP/NCP-induced bone formation. The quantity of bovine or human BMP/NCP-induced bone formation in rabbits is also increased by very low-dose immunosuppression but not by bone mineral, tricalcium phosphate ceramic, inorganic calcium salts, or various space-occupying, unspecific chemical irritants. Although composities of BMP/NCP and allogeneic rabbit tendon collagen increased the quantity of bone in a parosteal site, in a heterotopic site the composite failed to induce bone formation. In a parosteal site, the conditions permitting BMP/NCP-induced bone formation develop, and the end product of the morphogenetic response is a duplicate diaphysis. How BMP reactivates the morphogenetic process in postfetal mesenchymal-type adventitial cells persisting in adult periosteum (including adjacent muscle attachments) is not known.

Living in the matrix: assembly and control of Vibrio cholerae biofilms

PubMed Central

Teschler, Jennifer K.; Zamorano-Sánchez, David; Utada, Andrew S.; Warner, Christopher J. A.; Wong, Gerard C. L.; Linington, Roger G.; Yildiz, Fitnat H.

2015-01-01

Preface Nearly all bacteria form biofilms as a strategy for survival and persistence. Biofilms are associated with biotic and abiotic surfaces and are composed of aggregates of cells that are encased by a self-produced or acquired extracellular matrix. Vibrio cholerae has been studied as a model organism for understanding biofilm formation in environmental pathogens, as it spends much of its life cycle outside of the human host in the aquatic environment. Given the important role of biofilm formation in the V. cholerae life cycle, the molecular mechanisms underlying this process and the signals that trigger biofilm assembly or dispersal have been areas of intense investigation over the past 20 years. In this Review, we discuss V. cholerae surface attachment, various matrix components and the regulatory networks controlling biofilm formation. PMID:25895940

Foamy Macrophages from Tuberculous Patients' Granulomas Constitute a Nutrient-Rich Reservoir for M. tuberculosis Persistence

PubMed Central

Poquet, Yannick; Levillain, Florence; Botanch, Catherine; Bardou, Fabienne; Daffé, Mamadou; Emile, Jean-François; Marchou, Bruno; Cardona, Pere-Joan; de Chastellier, Chantal; Altare, Frédéric

2008-01-01

Tuberculosis (TB) is characterized by a tight interplay between Mycobacterium tuberculosis and host cells within granulomas. These cellular aggregates restrict bacterial spreading, but do not kill all the bacilli, which can persist for years. In-depth investigation of M. tuberculosis interactions with granuloma-specific cell populations are needed to gain insight into mycobacterial persistence, and to better understand the physiopathology of the disease. We have analyzed the formation of foamy macrophages (FMs), a granuloma-specific cell population characterized by its high lipid content, and studied their interaction with the tubercle bacillus. Within our in vitro human granuloma model, M. tuberculosis long chain fatty acids, namely oxygenated mycolic acids (MA), triggered the differentiation of human monocyte-derived macrophages into FMs. In these cells, mycobacteria no longer replicated and switched to a dormant non-replicative state. Electron microscopy observation of M. tuberculosis–infected FMs showed that the mycobacteria-containing phagosomes migrate towards host cell lipid bodies (LB), a process which culminates with the engulfment of the bacillus into the lipid droplets and with the accumulation of lipids within the microbe. Altogether, our results suggest that oxygenated mycolic acids from M. tuberculosis play a crucial role in the differentiation of macrophages into FMs. These cells might constitute a reservoir used by the tubercle bacillus for long-term persistence within its human host, and could provide a relevant model for the screening of new antimicrobials against non-replicating persistent mycobacteria. PMID:19002241

Does Tyrosyl DNA Phosphodiesterase-2 Play a Role in Hepatitis B Virus Genome Repair?

PubMed Central

Boregowda, Rajeev; Sohn, Ji A.; Ledesma, Felipe Cortes; Caldecott, Keith W.; Seeger, Christoph; Hu, Jianming

2015-01-01

Hepatitis B virus (HBV) replication and persistence are sustained by a nuclear episome, the covalently closed circular (CCC) DNA, which serves as the transcriptional template for all viral RNAs. CCC DNA is converted from a relaxed circular (RC) DNA in the virion early during infection as well as from RC DNA in intracellular progeny nucleocapsids via an intracellular amplification pathway. Current antiviral therapies suppress viral replication but cannot eliminate CCC DNA. Thus, persistence of CCC DNA remains an obstacle toward curing chronic HBV infection. Unfortunately, very little is known about how CCC DNA is formed. CCC DNA formation requires removal of the virally encoded reverse transcriptase (RT) protein from the 5’ end of the minus strand of RC DNA. Tyrosyl DNA phosphodiesterase-2 (Tdp2) was recently identified as the enzyme responsible for cleavage of tyrosyl-5’ DNA linkages formed between topoisomerase II and cellular DNA. Because the RT-DNA linkage is also a 5’ DNA-phosphotyrosyl bond, it has been hypothesized that Tdp2 might be one of several elusive host factors required for CCC DNA formation. Therefore, we examined the role of Tdp2 in RC DNA deproteination and CCC DNA formation. We demonstrated Tdp2 can cleave the tyrosyl-minus strand DNA linkage using authentic HBV RC DNA isolated from nucleocapsids and using RT covalently linked to short minus strand DNA produced in vitro. On the other hand, our results showed that Tdp2 gene knockout did not block CCC DNA formation during HBV infection of permissive human hepatoma cells and did not prevent intracellular amplification of duck hepatitis B virus CCC DNA. These results indicate that although Tdp2 can remove the RT covalently linked to the 5’ end of the HBV minus strand DNA in vitro, this protein might not be required for CCC DNA formation in vivo. PMID:26079492

A new class of synthetic retinoid antibiotics effective against bacterial persisters.

PubMed

Kim, Wooseong; Zhu, Wenpeng; Hendricks, Gabriel Lambert; Van Tyne, Daria; Steele, Andrew D; Keohane, Colleen E; Fricke, Nico; Conery, Annie L; Shen, Steven; Pan, Wen; Lee, Kiho; Rajamuthiah, Rajmohan; Fuchs, Beth Burgwyn; Vlahovska, Petia M; Wuest, William M; Gilmore, Michael S; Gao, Huajian; Ausubel, Frederick M; Mylonakis, Eleftherios

2018-04-05

A challenge in the treatment of Staphylococcus aureus infections is the high prevalence of methicillin-resistant S. aureus (MRSA) strains and the formation of non-growing, dormant 'persister' subpopulations that exhibit high levels of tolerance to antibiotics and have a role in chronic or recurrent infections. As conventional antibiotics are not effective in the treatment of infections caused by such bacteria, novel antibacterial therapeutics are urgently required. Here we used a Caenorhabditis elegans-MRSA infection screen to identify two synthetic retinoids, CD437 and CD1530, which kill both growing and persister MRSA cells by disrupting lipid bilayers. CD437 and CD1530 exhibit high killing rates, synergism with gentamicin, and a low probability of resistance selection. All-atom molecular dynamics simulations demonstrated that the ability of retinoids to penetrate and embed in lipid bilayers correlates with their bactericidal ability. An analogue of CD437 was found to retain anti-persister activity and show an improved cytotoxicity profile. Both CD437 and this analogue, alone or in combination with gentamicin, exhibit considerable efficacy in a mouse model of chronic MRSA infection. With further development and optimization, synthetic retinoids have the potential to become a new class of antimicrobials for the treatment of Gram-positive bacterial infections that are currently difficult to cure.

Modeling antimicrobial tolerance and treatment of heterogeneous biofilms.

PubMed

Zhao, Jia; Seeluangsawat, Paisa; Wang, Qi

2016-12-01

A multiphasic, hydrodynamic model for spatially heterogeneous biofilms based on the phase field formulation is developed and applied to analyze antimicrobial tolerance of biofilms by acknowledging the existence of persistent and susceptible cells in the total population of bacteria. The model implements a new conversion rate between persistent and susceptible cells and its homogeneous dynamics is bench-marked against a known experiment quantitatively. It is then discretized and solved on graphic processing units (GPUs) in 3-D space and time. With the model, biofilm development and antimicrobial treatment of biofilms in a flow cell are investigated numerically. Model predictions agree qualitatively well with available experimental observations. Specifically, numerical results demonstrate that: (i) in a flow cell, nutrient, diffused in solvent and transported by hydrodynamics, has an apparent impact on persister formation, thereby antimicrobial persistence of biofilms; (ii) dosing antimicrobial agents inside biofilms is more effective than dosing through diffusion in solvent; (iii) periodic dosing is less effective in antimicrobial treatment of biofilms in a nutrient deficient environment than in a nutrient sufficient environment. This model provides us with a simulation tool to analyze mechanisms of biofilm tolerance to antimicrobial agents and to derive potentially optimal dosing strategies for biofilm control and treatment. Copyright © 2016 Elsevier Inc. All rights reserved.

Social Capital in Schools: A Conceptual and Empirical Analysis of the Equity of Its Distribution and Relation to Academic Achievement

ERIC Educational Resources Information Center

Salloum, Serena J.; Goddard, Roger D.; Larsen, Ross

2017-01-01

Background: Schools face pressure to promote equitable student outcomes as the achievement gap continues to persist. The authors examine different ways in which social capital has been conceptualized as well as prior theory and research on its formation and consequences. While some theoretical and empirical work conceptualizes social capital as a…

The formation of dense understory layers in forests worldwide: consequences and implications for forest dynamics, biodiversity, and succession

Treesearch

Alejandro A. Royo; Walter P. Carson

2010-01-01

Alterations to natural herbivore and disturbance regimes often allow a select suite of forest understory plant species to dramatically spread and form persistent, mono-dominant thickets. Following their expansion, this newly established understory canopy can alter tree seedling recruitment rates and exert considerable control over the rate and direction of secondary...

The formation of dense understory layers in the forest worldwide: consequences and implications for forest dynamics, biodiversity, and succession

Treesearch

Alejandro A. Royo; Walter P. Carson

2010-01-01

Alterations to natural herbivore and disturbance regimes often allow a select suite of forest understory plant species to dramatically spread and form persistent, mono-dominant thickets. Following their expansion, this newly established understory canopy can alter tree seedling recruitment rates and exert considerable control over the rate and direction of secondary...

PATIKAweb: a Web interface for analyzing biological pathways through advanced querying and visualization.

PubMed

Dogrusoz, U; Erson, E Z; Giral, E; Demir, E; Babur, O; Cetintas, A; Colak, R

2006-02-01

Patikaweb provides a Web interface for retrieving and analyzing biological pathways in the Patika database, which contains data integrated from various prominent public pathway databases. It features a user-friendly interface, dynamic visualization and automated layout, advanced graph-theoretic queries for extracting biologically important phenomena, local persistence capability and exporting facilities to various pathway exchange formats.

Investigating Metabolic Control of Persister Formation in Biofilms

DTIC Science & Technology

2013-10-01

References Albe, K.R., Butler, M.H., and Wright, B.E. (1990). Cellular concentrations of enzymes and their substrates. Journal of theoretical biology 143 ...0.00 1,2- Propanediol 0.00±0.00 Tween 40 0.25±0.04 α- Keto - Glutaric Acid 0.26±0.07 α- Keto -Butyric Acid 0.18±0.03 α-Methyl-D

On the formation and persistence of superfog in woodland smoke

Treesearch

G.L. Achtemeier

2009-01-01

Dense fogs, comparable to historical fogs in England, have been implicated in numerous roadway accidents in the southern United States. Many of the fogs have occurred in association with prescribed burning. Direct measurements of superfog (fog reducing visibility to less than 3 m) were taken during burning of forest litter on 22 March 2003. Visibility was measured at 0...

Role of curli and cellulose expression by Escherichia coli O157:H7 on the cell’s ability to attach to spinach

USDA-ARS?s Scientific Manuscript database

Shiga-toxigenic Escherichia coli O157:H7 (STEC) outbreaks have been linked to consumption of fresh produce. Cellular appendages, such as curli fibers have been suggested to be involved in STEC persistence in fresh produce as these curli are critical in biofilm formation and adherence to animal cell...

On the formation and persistence of superfog in woodland smoke

Treesearch

Gary L. Achtemeier

2009-01-01

Dense fogs. comparable to historical fogs in England, have been implicated in numerous roadway accidents in the southern United States. Many of the fogs have occurred in association with prescribed burning. Direct measurements of superfog (fog reducing visibility to less than 3 rn) were taken during burning of forest litter on 22 March 2003. Visibility was measured at...

Loose, Falling Characters and Sentences: The Persistence of the OCR Problem in Digital Repository E-Books

ERIC Educational Resources Information Center

Kichuk, Diana

2015-01-01

The electronic conversion of scanned image files to readable text using optical character recognition (OCR) software and the subsequent migration of raw OCR text to e-book text file formats are key remediation or media conversion technologies used in digital repository e-book production. Despite real progress, the OCR problem of reliability and…

Knowing Where They Went: Six Years of Online Access Statistics via the Online Catalog for Federal Government Information

ERIC Educational Resources Information Center

Brown, Christopher C.

2011-01-01

As federal government information is increasingly migrating to online formats, libraries are providing links to this content via URLs or persistent URLs (PURLs) in their online public access catalogs (OPACs). Clickthrough statistics that accumulated as users visited links to online content in the University of Denver's library OPAC were gathered…

Acute prostatitis in middle-aged men: a prospective study.

PubMed

Kravchick, S; Cytron, S; Agulansky, L; Ben-Dor, D

2004-01-01

To determine the clinical outcome of middle-aged men with acute prostatitis, the optimum time for re-assessing their prostate-specific antigen (PSA) levels, and to detect any possible echotextural and vascular changes that remain as a consequence of acute inflammation. Persistent fever prompted a re-evaluation for prostatic abscess formation in 28 middle-aged men, using transrectal ultrasonography (TRUS) colour Doppler imaging, undertaken at the 3-, 6- and 12-month visits. The results of TRUS were compared with laboratory data and clinical outcome. Two abscesses were detected; 19 (68%) of the patients remained infection-free at the 3-month visit. Serum PSA levels were elevated in 11 (39%) of the patients at this visit; three prostate carcinomas were diagnosed. Increased intraprostatic colour flow was detected in 68% and there were hypoechoic areas in 46% of the patients. The re-evaluation for abscess formation should not be postponed for > 48 h. Patients with acute prostatitis tend to have persistent infection. PSA levels could be high even up to 3 months after an acute episode. Middle-aged men with carcinoma could be missed during the acute phase of inflammation. PSA and TRUS monitoring are strongly recommended.

Turbulence and fossil turbulence lead to life in the universe

NASA Astrophysics Data System (ADS)

Gibson, Carl H.

2013-07-01

Turbulence is defined as an eddy-like state of fluid motion where the inertial-vortex forces of the eddies are larger than all the other forces that tend to damp the eddies out. Fossil turbulence is a perturbation produced by turbulence that persists after the fluid ceases to be turbulent at the scale of the perturbation. Because vorticity is produced at small scales, turbulence must cascade from small scales to large, providing a consistent physical basis for Kolmogorovian universal similarity laws. Oceanic and astrophysical mixing and diffusion are dominated by fossil turbulence and fossil turbulent waves. Observations from space telescopes show turbulence and vorticity existed in the beginning of the universe and that their fossils persist. Fossils of big bang turbulence include spin and the dark matter of galaxies: clumps of ∼1012 frozen hydrogen planets that make globular star clusters as seen by infrared and microwave space telescopes. When the planets were hot gas, they hosted the formation of life in a cosmic soup of hot-water oceans as they merged to form the first stars and chemicals. Because spontaneous life formation according to the standard cosmological model is virtually impossible, the existence of life falsifies the standard cosmological model.

Turbulence and Fossil Turbulence lead to Life in the Universe

NASA Astrophysics Data System (ADS)

Gibson, Carl H.

2012-03-01

Turbulence is defined as an eddy-like state of fluid motion where the inertial-vortex forces of the eddies are larger than all the other forces that tend to damp the eddies out. Fossil turbulence is a perturbation produced by turbulence that persists after the fluid ceases to be turbulent at the scale of the perturbation. Because vorticity is produced at small scales, turbulence must cascade from small scales to large, providing a consistent physical basis for Kolmogorovian universal similarity laws. Oceanic and astrophysical mixing and diffusion are dominated by fossil turbulence and fossil turbulent waves. Observations from space telescopes show turbulence and vorticity existed in the beginning of the universe and that their fossils persist. Fossils of big bang turbulence include spin and the dark matter of galaxies: clumps of ~ 1012 frozen hydrogen planets that make globular star clusters as seen by infrared and microwave space telescopes. When the planets were hot gas, they hosted the formation of life in a cosmic soup of hot- water oceans as they merged to form the first stars and chemicals. Because spontaneous life formation according to the standard cosmological model is virtually impossible, the existence of life falsifies the standard cosmological model.

Germination traits explain soil seed persistence across species: the case of Mediterranean annual plants in cereal fields

PubMed Central

Saatkamp, Arne; Affre, Laurence; Dutoit, Thierry; Poschlod, Peter

2011-01-01

Background and Aims Seed persistence in the soil under field conditions is an important issue for the maintenance of local plant populations and the restoration of plant communities, increasingly so in the light of rapidly changing land use and climate change. Whereas processes important for dispersal in space are well known, knowledge of processes governing dispersal in time is still limited. Data for morphological seed traits such as size have given contradictory results for prediction of soil seed persistence or cover only a few species. There have been few experimental studies on the role of germination traits in determining soil seed persistence, while none has studied their predictive value consistently across species. Delayed germination, as well as light requirements for germination, have been suggested to contribute to the formation of persistent seed banks. Moreover, diurnally fluctuating temperatures can influence the timing of germination and are therefore linked to seed bank persistence. Methods The role of germination speed measured by T50 (days to germination of 50 % of all germinated seeds), light requirement and reaction to diurnally fluctuating temperatures in determining seed persistence in the soil was evaluated using an experimental comparative data set of 25 annual cereal weed species. Key Results It is shown that light requirements and slow germination are important features to maintain seeds ungerminated just after entering the soil, and hence influence survival of seeds in the soil. However, the detection of low diurnally fluctuating temperatures enhances soil seed bank persistence by limiting germination. Our data further suggest that the effect of diurnally fluctuating temperatures, as measured on seeds after dispersal and dry storage, is increasingly important to prevent fatal germination after longer burial periods. Conclusions These results underline the functional role of delayed germination and light for survival of seeds in the soil and hence their importance for shaping the first part of the seed decay curve. Our analyses highlight the detection of diurnally fluctuating temperatures as a third mechanism to achieve higher soil seed persistence after burial which interacts strongly with season. We therefore advocate focusing future research on mechanisms that favour soil seed persistence after longer burial times and moving from studies of morphological features to exploration of germination traits such as reaction to diurnally fluctuating temperatures. PMID:21224268

The sources, fate, and toxicity of chemical warfare agent degradation products.

PubMed Central

Munro, N B; Talmage, S S; Griffin, G D; Waters, L C; Watson, A P; King, J F; Hauschild, V

1999-01-01

We include in this review an assessment of the formation, environmental fate, and mammalian and ecotoxicity of CW agent degradation products relevant to environmental and occupational health. These parent CW agents include several vesicants: sulfur mustards [undistilled sulfur mustard (H), sulfur mustard (HD), and an HD/agent T mixture (HT)]; nitrogen mustards [ethylbis(2-chloroethyl)amine (HN1), methylbis(2-chloroethyl)amine (HN2), tris(2-chloroethyl)amine (HN3)], and Lewisite; four nerve agents (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate (VX), tabun (GA), sarin (GB), and soman (GD)); and the blood agent cyanogen chloride. The degradation processes considered here include hydrolysis, microbial degradation, oxidation, and photolysis. We also briefly address decontamination but not combustion processes. Because CW agents are generally not considered very persistent, certain degradation products of significant persistence, even those that are not particularly toxic, may indicate previous CW agent presence or that degradation has occurred. Of those products for which there are data on both environmental fate and toxicity, only a few are both environmentally persistent and highly toxic. Major degradation products estimated to be of significant persistence (weeks to years) include thiodiglycol for HD; Lewisite oxide for Lewisite; and ethyl methyl phosphonic acid, methyl phosphonic acid, and possibly S-(2-diisopropylaminoethyl) methylphosphonothioic acid (EA 2192) for VX. Methyl phosphonic acid is also the ultimate hydrolysis product of both GB and GD. The GB product, isopropyl methylphosphonic acid, and a closely related contaminant of GB, diisopropyl methylphosphonate, are also persistent. Of all of these compounds, only Lewisite oxide and EA 2192 possess high mammalian toxicity. Unlike other CW agents, sulfur mustard agents (e.g., HD) are somewhat persistent; therefore, sites or conditions involving potential HD contamination should include an evaluation of both the agent and thiodiglycol. Images Figure 1 Figure 3 Figure 5 PMID:10585900

Characterization of Air Plane Soot Surrogates using Raman spectroscopy and laser ablation techniques

NASA Astrophysics Data System (ADS)

Chazallon, Bertrand; Ortega, Ismael Kenneth; Ikhenazene, Raouf; Pirim, Claire; Carpentier, Yvain; Irimiea, Cornelia; Focsa, Cristian; Ouf, François-Xavier

2016-04-01

Aviation alters the composition of the atmosphere globally and can thus drive climate change and ozone depletion [1]. Aircraft exhaust plumes contain species (gases and soot particles) produced by the combustion of kerosene with ambient air in the combustion chamber of the engine. Soot particles emitted by air-planes produce persistent contrails in the upper troposphere in ice-supersaturated air masses that contribute to cloudiness and impact the radiative properties of the atmosphere. These aerosol-cloud interactions represent one of the largest sources of uncertainty in global climate models [2]. Though the formation of atmospheric ice particles has been studied for many years [3], there are still numerous opened questions on nucleation properties of soot particles [4], as the ice nucleation experiments showed a large spread in results depending on the nucleation mode chosen and origin of the soot produced. The reasons behind these discrepancies reside in the different physico-chemical properties (composition, structure) of soot particles produced in different conditions, e.g., with respect to fuel or combustion techniques. In this work, we use Raman microscopy (514 and 785 nm excitation wavelengths) and ablation techniques (Secondary Ions Mass Spectrometry, and Laser Desorption Mass Spectrometry) to characterize soot particle surrogates produced from a CAST generator (propane fuel, four different global equivalence ratios). They are produced as analogues of air-plane soot collected at different engine regimes (PowerJet SaM-146 turbofan) simulating a landing and take-off (LTO) cycle (MERMOSE project (http://mermose.onera.fr/)) [6]. The spectral parameters of the first-order Raman bands of these soot samples are analyzed using a de-convolution approach described by Sadezky et al. (2005) [5]. A systematic Raman analysis is carried out to select a number of parameters (laser wavelength, irradiance at sample, exposure time) that will alter the sample and the resulting spectra. If much literature discussed the most appropriate values of these parameters for analyzing graphite, coals, or amorphous carbon, only few works have dealt with soot, and no published studies have proposed the optimal parameter values for air-plane soot surrogates. In this work, we present the effect of three Raman parameters (laser wavelength, irradiance at sample, exposure time) on air-plane soot surrogates. The obtained results qualitatively indicate higher reactivity of soot samples collected in specific air-oxidation conditions. The surface chemical composition of the soot particles with special focus on PAHs are analyzed by two-Step (Desorption/Ionization) Laser Mass Spectrometry (L2MS) and Time of Fight Secondary Ion Mass Spectrometry (ToF-SIMS) techniques. In both techniques the spectra are obtained using positive polarity, which is better suited for detection of PAHs. A good agreement was obtained between the two techniques for the total PAH content of the analyzed samples. CAST samples are further processed by Raman spectroscopy to get complementary information on their structure. References [1] Lee et al., Atmos. Env. 44, 4678-4734, 2010 [2] IPCC 2014, Chap7: http://www.ipcc.ch [3] L. Dufour, Ciel et Terre, vol 82, p1-36, 1966 [4] C. Hoose & O. Möhler, Atmos.Chem.Phys. 12, 9817-9854, 2012 [5] Sadezky, A., Muckenhuber, H., Grothe, H., Niessner, R. and Pöschl U., (2005) Carbon, 43, 1731-1742. [6] Parent, Laffon, Marhaba, Ferry, Regier, Ortega, Chazallon, Carpentier, Focsa, (2016) Carbon (accepted)

Effect of turpentine-induced fever during the enamel formation of rat incisor.

PubMed

Tung, Kuochung; Fujita, Haruko; Yamashita, Yasuo; Takagi, Yuzo

2006-06-01

Some epidemiological studies have indicated that diseases resulting in prolonged and sustained fever, such as exanthemata, respiratory infections and otitis media in infantile period or childhood are likely to have a marked deleterious effect on enamel formation, but the relationship between fever and enamel defects is unknown. The purpose of the present study was to induce a persistent high fever and examine the effects on the developing tooth enamel. Twenty male Wistar rats weighting 140+/-10 g were used in this study. For the experimental group, a dose of 2.3 ml/kg steam-distilled turpentine was subcutaneously injected into both hind limbs five times at 12h intervals. Control rats received 2.3 ml/kg of sterile saline into the same injection site. The rectal temperatures of animals were measured at the febrile period. After constant periods, the animals were sacrificed, and the mandibular incisors were examined by contact microradiography (CMR) and histological observation. The febrile state lasted for 57 h and the average temperature rose 1.51 degrees C higher than that of the control group. The ground sections, semi-thin and ultra-thin sections of mandibular incisors were prepared and the enamel was observed. The microradiographs showed a radiolucent line along with the incremental line in the enamel. Moreover, microscopic examination indicated disorientation of enamel prism and crystal-free area within this radiolucent lesion. Persistent high fever pattern was established firmly by the turpentine injections and the process of enamel formation was influenced by the febrile period.

Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation

PubMed Central

Hyppa, Randy W.; Benko, Zsigmond; Misova, Ivana; Schleiffer, Alexander; Smith, Gerald R.; Gregan, Juraj

2016-01-01

To identify new proteins required for faithful meiotic chromosome segregation, we screened a Schizosaccharomyces pombe deletion mutant library and found that deletion of the dbl2 gene led to missegregation of chromosomes during meiosis. Analyses of both live and fixed cells showed that dbl2Δ mutant cells frequently failed to segregate homologous chromosomes to opposite poles during meiosis I. Removing Rec12 (Spo11 homolog) to eliminate meiotic DNA double-strand breaks (DSBs) suppressed the segregation defect in dbl2Δ cells, indicating that Dbl2 acts after the initiation of meiotic recombination. Analyses of DSBs and Holliday junctions revealed no significant defect in their formation or processing in dbl2Δ mutant cells, although some Rec12-dependent DNA joint molecules persisted late in meiosis. Failure to segregate chromosomes in the absence of Dbl2 correlated with persistent Rad51 foci, and deletion of rad51 or genes encoding Rad51 mediators also suppressed the segregation defect of dbl2Δ. Formation of foci of Fbh1, an F-box helicase that efficiently dismantles Rad51-DNA filaments, was impaired in dbl2Δ cells. Our results suggest that Dbl2 is a novel regulator of Fbh1 and thereby Rad51-dependent DSB repair required for proper meiotic chromosome segregation and viable sex cell formation. The wide conservation of these proteins suggests that our results apply to many species. PMID:27304859

HDAC3 and the Molecular Brake Pad Hypothesis

PubMed Central

McQuown, Susan C.; Wood, Marcelo A.

2011-01-01

Successful transcription of specific genes required for long-term memory processes involves the orchestrated effort of not only transcription factors, but also very specific enzymatic protein complexes that modify chromatin structure. Chromatin modification has been identified as a pivotal molecular mechanism underlying certain forms of synaptic plasticity and memory. The best-studied form of chromatin modification in the learning and memory field is histone acetylation, which is regulated by histone acetyltransferases and histone deacetylases (HDACs). HDAC inhibitors have been shown to strongly enhance long-term memory processes, and recent work has aimed to identify contributions of individual HDACs. In this review, we focus on HDAC3 and discuss its recently defined role as a negative regulator of long-term memory formation. HDAC3 is part of a corepressor complex and has direct interactions with class II HDACs that may be important for its molecular and behavioral consequences. And last, we propose the “molecular brake pad” hypothesis of HDAC function. The HDACs and associated corepressor complexes may function in neurons, in part, as “molecular brake pads.” HDACs are localized to promoters of active genes and act as a persistent clamp that requires strong activity-dependent signaling to temporarily release these complexes (or brake pads) to activate gene expression required for long-term memory formation. Thus, HDAC inhibition removes the “molecular brake pads” constraining the processes necessary for long-term memory and results in strong, persistent memory formation. PMID:21521655

MHD and Reconnection Activity During Local Helicity Injection

NASA Astrophysics Data System (ADS)

Barr, J. L.; Bongard, M. W.; Burke, M. G.; Fonck, R. J.; Reusch, J. A.; Richner, N. J.

2016-10-01

Scaling local helicity injection (LHI) to larger devices requires a validated, predictive model of its current drive mechanism. NIMROD simulations predict the injected helical current streams persist in the edge and periodically reconnect to form axisymmetric current rings that travel into the bulk plasma to grow Ip and poloidal flux. In simulation, these events result in discrete bursts of Alfvénic-frequency MHD activity and jumps in Ip of order ΔIp Iinj , in qualitative agreement with large n = 1 activity found in experiment. Fast imaging prior to tokamak formation supports the instability of, and apparent reconnection between, adjacent helical streams. The bursts exhibit toroidal amplitude asymmetries consistent with a kink structure singly line-tied to the injectors. Internal measurements localize this activity to the injector radial location. Pairwise correlations of poloidal Mirnov coil amplitude and phase match expectations of an edge-localized current stream carrying Iinj. Prior to tokamak formation, reconnection from both adjacent helical windings and co-injected current streams are shown to strongly heat impurity ions. After tokamak formation, strong anomalous ion heating in the plasma edge is attributed to continuous reconnection between colinear streams. The n = 1 bursts occur less frequently as Ip rises, likely caused by increased stream stability as Bv rises and qedge drops. This evidence supports the general NIMROD model of LHI, confirms the persistence and role of the edge current streams, and motivates experiments at higher Iinj and BT. Supported by US DOE Grants DE-FG02-96ER54375, DE-SC0006928.

On critical behaviour in generalized Kadomtsev-Petviashvili equations

NASA Astrophysics Data System (ADS)

Dubrovin, B.; Grava, T.; Klein, C.

2016-10-01

An asymptotic description of the formation of dispersive shock waves in solutions to the generalized Kadomtsev-Petviashvili (KP) equation is conjectured. The asymptotic description based on a multiscales expansion is given in terms of a special solution to an ordinary differential equation of the Painlevé I hierarchy. Several examples are discussed numerically to provide strong evidence for the validity of the conjecture. The numerical study of the long time behaviour of these examples indicates persistence of dispersive shock waves in solutions to the (subcritical) KP equations, while in the supercritical KP equations a blow-up occurs after the formation of the dispersive shock waves.

Bone formation within a breast abscess.

PubMed

Mannu, Gurdeep Singh; Ahmed, Farid; Cunnick, Giles; Mungalsingh, Naren

2014-09-22

We present a rare case of osseous metaplasia in a poorly healing breast abscess. An 87-year-old woman was referred to the breast surgery clinic with a painful lump in her right breast. Initial imaging and core biopsy suggested a breast abscess. Despite several courses of antibiotics and repeated attempts at aspiration the painful lesion persisted. It was eventually surgically excised in its entirety and final histopathology showed the presence of bone formation within the abscess. The patient's symptoms subsequently resolved. To the best of our knowledge, this is the first case in the literature, of osseous metaplasia within a breast abscess in the absence of malignancy. 2014 BMJ Publishing Group Ltd.

Bone formation within a breast abscess

PubMed Central

Mannu, Gurdeep Singh; Ahmed, Farid; Cunnick, Giles; Mungalsingh, Naren

2014-01-01

We present a rare case of osseous metaplasia in a poorly healing breast abscess. An 87-year-old woman was referred to the breast surgery clinic with a painful lump in her right breast. Initial imaging and core biopsy suggested a breast abscess. Despite several courses of antibiotics and repeated attempts at aspiration the painful lesion persisted. It was eventually surgically excised in its entirety and final histopathology showed the presence of bone formation within the abscess. The patient's symptoms subsequently resolved. To the best of our knowledge, this is the first case in the literature, of osseous metaplasia within a breast abscess in the absence of malignancy. PMID:25246453

DOE Office of Scientific and Technical Information (OSTI.GOV)

L. Delgado-Aparicio, et. al.

New observations of the formation and dynamics of long-lived impurity-induced helical "snake" modes in tokamak plasmas have recently been carried-out on Alcator C-Mod. The snakes form as an asymmetry in the impurity ion density that undergoes a seamless transition from a small helically displaced density to a large crescent-shaped helical structure inside q < 1, with a regularly sawtoothing core. The observations show that the conditions for the formation and persistence of a snake cannot be explained by plasma pressure alone. Instead, many features arise naturally from nonlinear interactions in a 3D MHD model that separately evolves the plasma densitymore » and temperature« less

Predicted electric-field-induced hexatic structure in an ionomer membrane

NASA Astrophysics Data System (ADS)

Allahyarov, Elshad; Taylor, Philip L.

2009-08-01

Coarse-grained molecular-dynamics simulations were used to study the morphological changes induced in a Nafion®-like ionomer by the imposition of a strong electric field. We observe the formation of structures aligned along the direction of the applied field. The polar head groups of the ionomer sidechains aggregate into clusters, which then form rodlike formations which assemble into a hexatic array aligned with the direction of the field. Occasionally these lines of sulfonates and protons form a helical structure. Upon removal of the electric field, the hexatic array of rodlike structures persists and has a lower calculated free energy than the original isotropic morphology.

Social Memory Formation Rapidly and Differentially Affects the Motivation and Performance of Vocal Communication Signals in the Bengalese Finch (Lonchura striata var. domestica)

PubMed Central

Toccalino, Danielle C.; Sun, Herie; Sakata, Jon T.

2016-01-01

Cognitive processes like the formation of social memories can shape the nature of social interactions between conspecifics. Male songbirds use vocal signals during courtship interactions with females, but the degree to which social memory and familiarity influences the likelihood and structure of male courtship song remains largely unknown. Using a habituation-dishabituation paradigm, we found that a single, brief (<30 s) exposure to a female led to the formation of a short-term memory for that female: adult male Bengalese finches were significantly less likely to produce courtship song to an individual female when re-exposed to her 5 min later (i.e., habituation). Familiarity also rapidly decreased the duration of courtship songs but did not affect other measures of song performance (e.g., song tempo and the stereotypy of syllable structure and sequencing). Consistent with a contribution of social memory to the decrease in courtship song with repeated exposures to the same female, the likelihood that male Bengalese finches produced courtship song increased when they were exposed to a different female (i.e., dishabituation). Three consecutive exposures to individual females also led to the formation of a longer-term memory that persisted over days. Specifically, when courtship song production was assessed 2 days after initial exposures to females, males produced fewer and shorter courtship songs to familiar females than to unfamiliar females. Measures of song performance, however, were not different between courtship songs produced to familiar and unfamiliar females. The formation of a longer-term memory for individual females seemed to require at least three exposures because males did not differentially produce courtship song to unfamiliar females and females that they had been exposed to only once or twice. Taken together, these data indicate that brief exposures to individual females led to the rapid formation and persistence of social memories and support the existence of distinct mechanisms underlying the motivation to produce and the performance of courtship song. PMID:27378868

A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm.

PubMed

Motomura, Kazuki; Kawashima, Tomokazu; Berger, Frédéric; Kinoshita, Tetsu; Higashiyama, Tetsuya; Maruyama, Daisuke

2018-01-29

Cell fusion is a pivotal process in fertilization and multinucleate cell formation. A plant cell is ubiquitously surrounded by a hard cell wall, and very few cell fusions have been observed except for gamete fusions. We recently reported that the fertilized central cell (the endosperm) absorbs the persistent synergid, a highly differentiated cell necessary for pollen tube attraction. The synergid-endosperm fusion (SE fusion) appears to eliminate the persistent synergid from fertilized ovule in Arabidopsis thaliana Here, we analyzed the effects of various inhibitors on SE fusion in an in vitro culture system. Different from other cell fusions, neither disruption of actin polymerization nor protein secretion impaired SE fusion. However, transcriptional and translational inhibitors decreased the SE fusion success rate and also inhibited endosperm division. Failures of SE fusion and endosperm nuclear proliferation were also induced by roscovitine, an inhibitor of cyclin-dependent kinases (CDK). These data indicate unique aspects of SE fusion such as independence of filamentous actin support and the importance of CDK-mediated mitotic control. © 2018. Published by The Company of Biologists Ltd.

Mesoporous TiO2/graphene composite films for the photocatalytic degradation of eco-persistent pollutants

NASA Astrophysics Data System (ADS)

Kusumawati, Yuly; Pauporté, Thierry; Viana, Bruno; Zouzelka, Radek; Remzova, Monika; Rathousky, Jiri

2017-03-01

Graphene/oxide composite structures are attracting increasing attention for many advanced applications. In the present work, mesoporous layers composed of TiO2 nanoparticles and graphene at various concentrations have been coated on conductive glass substrates. They have been tested for the photocatalytic degradation of 4-chlorophenol used as a model compound of an eco-persistent pollutant dilute in water. The formation of intermediate degradation products, namely, hydroquinone and benzoquinone, has been followed. The results show the high photocatalytic activity of the layers and a beneficial effect of graphene for an optimum concentration of 1.2 wt. %. The decrease in the activity observed at higher graphene content is assigned to the light absorption by this component. The key parameters for the enhancement of the photocatalytic performance are discussed.

A Smoking Gun in the Carina Nebula

NASA Technical Reports Server (NTRS)

Hamaguchi, Kenji; Corcoran, Michael F.; Ezoe, Yuichiro; Townsley, Leisa; Broos, Patrick; Gruendl, Robert; Vaidya, Kaushar; White, Stephen M.; Petre, Rob; Chu, You-Hua

2009-01-01

The Carina Nebula is one of thc youngest, most active sites of massive star formation in our Galaxy. In this nebula, we have discovered a bright X-ray source that has persisted for approx.30 years. The soft X-ray spectrum. consistent with kT approx.130 eV blackbody radiation with mild extinction, and no counterpart in the near- and mid-infrared wavelengths indicate that it is a, approx. 10(exp 6)-year-old neutron star housed in the Carina Nebula. Current star formation theory does not suggest that the progenitor of the neutron star and massive stars in the Carina Nebula, in particular (eta)Car, are coeval. This result demonstrates that the Carina Nebula experienced at least two major episodes of massive star formation. The neutron star would be responsible for remnants of high energy activity seen in multiple wavelengths.

Neural and Cellular Mechanisms of Fear and Extinction Memory Formation

PubMed Central

Orsini, Caitlin A.; Maren, Stephen

2012-01-01

Over the course of natural history, countless animal species have evolved adaptive behavioral systems to cope with dangerous situations and promote survival. Emotional memories are central to these defense systems because they are rapidly acquired and prepare organisms for future threat. Unfortunately, the persistence and intrusion of memories of fearful experiences are quite common and can lead to pathogenic conditions, such as anxiety and phobias. Over the course of the last thirty years, neuroscientists and psychologists alike have attempted to understand the mechanisms by which the brain encodes and maintains these aversive memories. Of equal interest, though, is the neurobiology of extinction memory formation as this may shape current therapeutic techniques. Here we review the extant literature on the neurobiology of fear and extinction memory formation, with a strong focus on the cellular and molecular mechanisms underlying these processes. PMID:22230704

Early elective colostomy following spinal cord injury.

PubMed

Boucher, Michelle

Elective colostomy is an accepted method of bowel management for patients who have had a spinal cord injury (SCI). Approximately 2.4% of patients with SCI have a colostomy, and traditionally it is performed as a last resort several years after injury, and only if bowel complications persist when all other methods have failed. This is despite evidence that patients find a colostomy easier to manage and frequently report wishing it had been performed earlier. It was noticed in the author's spinal unit that increasing numbers of patients were requesting colostomy formation during inpatient rehabilitation following SCI. No supporting literature was found for this; it appears to be an emerging and untested practice. This article explores colostomy formation as a method of bowel management in patients with SCI, considers the optimal time for colostomy formation after injury and examines issues for health professionals.

Drivers of Plankton Patch Formation, Persistence and Decline in East Sound, Orcas Island, Washington

DTIC Science & Technology

2012-09-30

processes, including phytoplankton growth rates, carbon to Chl a ratios (Welschmeyer & Lorenzen 1984), heterotrophic protist grazing rates (Montagnes...dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography 45: 569-579 Menden-Deuer S, EJ Lessard & J Satterberg. 2001. Effect of...nutrient availability on measurements of heterotrophic protist grazing rates in Narragansett Bay, Rhode Island, USA. ASLO Aquatic Sciences Meeting

Drivers of Plankton Patch Formation, Persistence and Decline in East Sound, Orcas Island, Washington

DTIC Science & Technology

2011-09-30

protist grazing rates (Montagnes & Lessard 1999), and cellular carbon content (Putt & Stoecker 1989, Crawford & Stoecker 1996). The radiolabeling...Lessard. 2000. Carbon to volume relationships for dinoflagellates, diatoms, and other protist plankton. Limnology and Oceanography 45: 569-579 Menden...2011. Effects of copepod addition and nutrient availability on measurements of heterotrophic protist grazing rates in Narragansett Bay, Rhode Island

Exopolysaccharide Productivity and Biofilm Phenotype on Oral Commensal Bacteria as Pathogenesis of Chronic Periodontitis

DTIC Science & Technology

2012-01-01

2 Exopolysaccharide Productivity and Biofilm Phenotype on Oral Commensal Bacteria as Pathogenesis of Chronic Periodontitis Takeshi Yamanaka1...species biofilm in the oral cavity can cause persistent chronic periodontitis along with the importance of dental plaque formation and maturation...independent manner could be pathogenic for periodontal tissues and can cause chronic periodontitis lesions. 2.1 Initial colonizers on the tooth surface

DNA in soil: adsorption, genetic transformation, molecular evolution and genetic microchip.

PubMed

Trevors, J T

1996-07-01

This review examines interactions between DNA and soil with an emphasis on the persistence and stability of DNA in soil. The role of DNA in genetic transformation in soil microorganisms will also be discussed. In addition, a postulated mechanism for stabilization and elongation/assembly of primitive genetic material and the role of soil particles, salt concentrations, temperature cycling and crystal formation is examined.

PKA and PKC Are Required for Long-Term but Not Short-Term in Vivo Operant Memory in "Aplysia"

ERIC Educational Resources Information Center

Michel, Maximilian; Green, Charity L.; Lyons, Lisa C.

2011-01-01

We investigated the involvement of PKA and PKC signaling in a negatively reinforced operant learning paradigm in "Aplysia", learning that food is inedible (LFI). In vivo injection of PKA or PKC inhibitors blocked long-term LFI memory formation. Moreover, a persistent phase of PKA activity, although not PKC activity, was necessary for long-term…

Quorum signaling and sensing by nontypeable Haemophilus influenzae.

PubMed

Swords, W Edward

2012-01-01

Quorum signals are diffusible factors produced by bacteria that coordinate communal responses. For nontypeable Haemophilus influenzae (NTHi), a series of recent papers indicate that production and sensing of quorum signals are determinants of biofilm formation/maturation and persistence in vivo. In this mini-review I will summarize the current knowledge about quorum signaling/sensing by this organism, and identify specific topics for additional study.

Quorum signaling and sensing by nontypeable Haemophilus influenzae

PubMed Central

Swords, W. Edward

2012-01-01

Quorum signals are diffusible factors produced by bacteria that coordinate communal responses. For nontypeable Haemophilus influenzae (NTHi), a series of recent papers indicate that production and sensing of quorum signals are determinants of biofilm formation/maturation and persistence in vivo. In this mini-review I will summarize the current knowledge about quorum signaling/sensing by this organism, and identify specific topics for additional study. PMID:22919689

Large-Scale Antecedent Conditions Associated with 2014-2015 Winter Onset over North America and mid-Winter Storminess Along the North Atlantic Coast

NASA Astrophysics Data System (ADS)

Bosart, L. F.; Papin, P. P.; Bentley, A. M.; Benjamin, M.; Winters, A. C.

2015-12-01

Winter 2014-2015 was marked by the coldest November weather in 35 years east of the Rockies and record-breaking snowstorms and cold from the eastern Great Lakes to Atlantic Canada in January and February 2015. Record-breaking warmth prevailed across the Intermountain West and Rockies beneath a persistent upper-level ridge. Winter began with a series of arctic air mass surges that culminated in an epic lake-effect snowstorm occurred over western New York before Thanksgiving and was followed by a series of snow and ice storms that disrupted Thanksgiving holiday travel widely. Winter briefly abated in part of December, but returned with a vengeance between mid-January and mid-February 2015 when multiple extreme weather events that featured record-breaking monthly and seasonal snowfalls and record-breaking daily minimum temperatures were observed. This presentation will show how: (1) the recurvature and extratropical transition (ET) of Supertyphoon (STY) Nuri in the western Pacific in early November 2014, and its subsequent explosive reintensification as an extratropical cyclone (EC), disrupted the North Pacific jet stream and downstream Northern Hemisphere (NH) circulation, produced high-latitude ridging and the formation of an omega block over western North America, triggered downstream baroclinic development and the formation of a deep trough over eastern North America, and ushered in winter 2014-2015, (2) the ET/EC of STY Nuri increased subsequent week two predictability over the North Pacific and North America in association with diabatically influenced high-latitude ridge building, and (3) the amplification of the large-scale NH flow pattern beginning in January 2015 resulted in the formation of persistent high-amplitude ridges over northeastern Russia, Alaska, western North America, and the North Atlantic while deep troughs formed over the eastern North Pacific and eastern North America. This persistent amplified flow pattern supported the occurrence of frequent heavy snowstorms, including blizzards, over parts of the northeastern United States and adjacent Atlantic Canada during the latter part of January and much of February 2015.

Body mass index modulates aromatic DNA adduct levels and their persistence in smokers.

PubMed

Godschalk, Roger W L; Feldker, Dorien E M; Borm, Paul J A; Wouters, Emiel F M; van Schooten, Frederik-Jan

2002-08-01

Smokers with a low body mass index (BMI; weight/height(2)) have a higher risk for developing lung malignancies as compared with smokers of average weight, but there is no mechanistic explanation for this observation. Carcinogens in cigarette smoke are thought to elicit cancer by the formation of DNA adducts, which give the opportunity to additionally investigate the biological link between BMI and lung cancer. DNA adduct levels in peripheral blood lymphocytes of 24 healthy smoking volunteers (0.76 +/- 0.41 adducts per 10(8) nucleotides) positively correlated with cigarette consumption (r = 0.51; P = 0.01) and were inversely related with BMI (r = -0.48; P = 0.02). A significant overall relationship was observed when both parameters were included in multiple regression analysis (r = 0.63; P = 0.007). Moreover, body composition may affect DNA adduct persistence, because lipophilic tobacco smoke-derived carcinogens accumulate in adipose tissue and can be mobilized once exposure ceases. Therefore, DNA adduct levels and BMI were reassessed in all of the subjects after a nonsmoking period of 22 weeks. Adduct levels declined to 0.44 +/- 0.23 per 10(8) nucleotides (P = 0.002), and the estimated half-life was 11 weeks on the basis of exponential decay to background levels in never-smoking controls (0.33 +/- 0.18 per 10(8) nucleotides). Overweight subjects (BMI >25) with little weight gain after smoking cessation (

Introduction to the SONEX (Subsonic Assessment Ozone and Nitrogen Oxides Experiment) and POLINAT-2 (Pollution from Aircraft Emissions in the North Atlantic Flight Corridor) Special Issue

NASA Technical Reports Server (NTRS)

Thompson, Anne M.; Singh, Hanwant B.; Schlager, Hans; Einaudi, Franco (Technical Monitor)

2000-01-01

Emissions of atmospheric species from the engines of subsonic aircraft at cruise altitude (roughly, above seven kilometers) are of concern to scientists, the aviation industry and policymakers for two reasons. First, water vapor, soot and sulfur oxides, and related heterogeneous processes, may modify clouds and aerosols enough to perturb radiative forcing in the UT/LS (upper troposphere/lower stratosphere). A discussion of these phenomena appears in Chapter 3 of the IPCC Aviation Assessment (1999). An airborne campaign conducted to evaluate aviation effects on contrail, cirrus and cloud formation, is described in Geophysical Research Letters. The second concern arises from subsonic aircraft emissions of nitrogen oxides (NO + NO2 = NO(sub x)), CO, and hydrocarbons. These species may add to the background mixture of photochemically reactive species that form ozone. In the UT/LS, ozone is a highly effective greenhouse gas. The impacts of subsonic aircraft emissions on tropospheric NO(sub x) and ozone budgets have been studied with models that focus on UT chemistry [e.g. see discussions of individual models in Brasseur et al., 1998; Friedl et al., 1997; IPCC, 1999]. Depending on the model used, projected increases in the global subsonic aircraft fleet from 1992 to 2015 will lead to a 50-100 pptv increase in UT/LS NO. at 12 km (compared to 50-150 pptv background) in northern hemisphere midlatitudes. The corresponding 12-km ozone increase is 7-11 ppbv, or 5-10% (Chapter 4 in IPCC, 1999). Two major sources of uncertainties in model estimates of aviation effects are: (1) the often limited degree to which global models - the scale required to evaluate aircraft emissions - realistically simulate atmospheric transport and other physical processes; (2) limited UT/LS observations of trace gases with which to evaluate model performance. In response to the latter deficiency, a number of airborne campaigns aimed at elucidating the effect of aircraft on atmospheric nitrogen oxides and ozone were performed between 1990 and 1996 (see descriptions in Friedl et al., 1997; Brasseur et al., 1998).

Coccoliths in the Celtic Sea

NASA Technical Reports Server (NTRS)

2002-01-01

As the basis of the marine food chain, phytoplankton are important indicators of change in the oceans. These marine flora also extract carbon dioxide from the atmosphere for use in photosynthesis, and play an important role in global climate. Phytoplankton blooms that occur near the surface are readily visible from space, enabling a global estimation of the presence of chlorophyll and other pigments. There are more than 5,000 different species of phytoplankton however, and it is not always possible to identify the type of phytoplankton present using space-based remote sensing.

Coccolithophores, however, are a group of phytoplankton that are identifiable from space. These microscopic plants armor themselves with external plates of calcium carbonate. The plates, or coccoliths, give the ocean a milky white or turquoise appearance during intense blooms. The long-term flux of coccoliths to the ocean floor is the main process responsible for the formation of chalk and limestone.

This image is a natural-color view of the Celtic Sea and English Channel regions, and was acquired by the Multi-angle Imaging SpectroRadiometer's nadir (vertical-viewing) camera on June 4, 2001 during Terra orbit 7778. It represents an area of 380 kilometers x 445 kilometers, and includes portions of southwestern England and northwestern France. The coccolithophore bloom in the lower left-hand corner usually occurs in the Celtic Sea for several weeks in summer. The coccoliths backscatter light from the water column to create a bright optical effect. Other algal and/or phytoplankton blooms can also be discerned along the coasts near Portsmouth, England and Granville, France.

At full resolution, evidence of human activity is also apparent in this image. White specks associated with ship wakes are present in the open water, and aircraft contrails are visible within the high cirrus clouds over the English Channel.

MISR was built and is managed by NASA's Jet Propulsion Laboratory, Pasadena, CA, for NASA's Office of Earth Science, Washington, DC. The Terra satellite is managed by NASA's Goddard Space Flight Center, Greenbelt, MD. JPL is a division of the California Institute of Technology.

Analytical Tools for Cloudscope Ice Measurement

NASA Technical Reports Server (NTRS)

Arnott, W. Patrick

1998-01-01

The cloudscope is a ground or aircraft instrument for viewing ice crystals impacted on a sapphire window. It is essentially a simple optical microscope with an attached compact CCD video camera whose output is recorded on a Hi-8 mm video cassette recorder equipped with digital time and date recording capability. In aircraft operation the window is at a stagnation point of the flow so adiabatic compression heats the window to sublimate the ice crystals so that later impacting crystals can be imaged as well. A film heater is used for ground based operation to provide sublimation, and it can also be used to provide extra heat for aircraft operation. The compact video camera can be focused manually by the operator, and a beam splitter - miniature bulb combination provide illumination for night operation. Several shutter speeds are available to accommodate daytime illumination conditions by direct sunlight. The video images can be directly used to qualitatively assess the crystal content of cirrus clouds and contrails. Quantitative size spectra are obtained with the tools described in this report. Selected portions of the video images are digitized using a PCI bus frame grabber to form a short movie segment or stack using NIH (National Institute of Health) Image software with custom macros developed at DRI. The stack can be Fourier transform filtered with custom, easy to design filters to reduce most objectionable video artifacts. Particle quantification of each slice of the stack is performed using digital image analysis. Data recorded for each particle include particle number and centroid, frame number in the stack, particle area, perimeter, equivalent ellipse maximum and minimum radii, ellipse angle, and pixel number. Each valid particle in the stack is stamped with a unique number. This output can be used to obtain a semiquantitative appreciation of the crystal content. The particle information becomes the raw input for a subsequent program (FORTRAN) that synthesizes each slice and separates the new from the sublimating particles. The new particle information is used to generate quantitative particle concentration, area, and mass size spectra along with total concentration, solar extinction coefficient, and ice water content. This program directly creates output in html format for viewing with a web browser.

Formation of halogenated organics during waste-water disinfection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Singer, P.C.; Brown, R.A.; Wiseman, J.F.

The research examined the formation of trihalomethanes (THMs) and total organic halides (TOX) during wastewater chlorination at three wastewater treatment plants in the central Piedmont of North Carolina. Secondary effluent samples were collected before and after the addition of chlorine at each of the three treatment facilities; chlorinated samples were taken from various locations within the chlorine contact chambers and at the plant discharge. Water samples were also collected upstream and downstream from two of the plant outfalls to determine the increase and persistence of THMs and TOX below each plant. TOX and THM formation was evaluated in terms ofmore » effluent wastewater quality (e.g., residual chemical oxygen demand, total organic carbon and ammonia concentration), chlorine dose, chlorine contacting system, methods of chlorine addition, and chlorine-to-ammonia ratio. The results showed that TOX was present in the unchlorinated wastewater and that additional TOX was formed immediately after chlorine addition. Small to insignificant amounts of THMS were detected. TOX formation did not increase with increasing contact time, due to the rapid depletion of free chlorine and the formation of combined chlorine in the chlorine contact chamber.« less

Persistent sulfate formation from London Fog to Chinese haze

PubMed Central

Wang, Gehui; Zhang, Renyi; Gomez, Mario E.; Yang, Lingxiao; Levy Zamora, Misti; Hu, Min; Lin, Yun; Peng, Jianfei; Guo, Song; Meng, Jingjing; Li, Jianjun; Cheng, Chunlei; Hu, Tafeng; Ren, Yanqin; Wang, Yuesi; Gao, Jian; Cao, Junji; An, Zhisheng; Zhou, Weijian; Li, Guohui; Wang, Jiayuan; Tian, Pengfei; Marrero-Ortiz, Wilmarie; Secrest, Jeremiah; Du, Zhuofei; Zheng, Jing; Shang, Dongjie; Zeng, Limin; Shao, Min; Wang, Weigang; Huang, Yao; Wang, Yuan; Zhu, Yujiao; Li, Yixin; Hu, Jiaxi; Pan, Bowen; Cai, Li; Cheng, Yuting; Ji, Yuemeng; Zhang, Fang; Rosenfeld, Daniel; Liss, Peter S.; Duce, Robert A.; Kolb, Charles E.; Molina, Mario J.

2016-01-01

Sulfate aerosols exert profound impacts on human and ecosystem health, weather, and climate, but their formation mechanism remains uncertain. Atmospheric models consistently underpredict sulfate levels under diverse environmental conditions. From atmospheric measurements in two Chinese megacities and complementary laboratory experiments, we show that the aqueous oxidation of SO2 by NO2 is key to efficient sulfate formation but is only feasible under two atmospheric conditions: on fine aerosols with high relative humidity and NH3 neutralization or under cloud conditions. Under polluted environments, this SO2 oxidation process leads to large sulfate production rates and promotes formation of nitrate and organic matter on aqueous particles, exacerbating severe haze development. Effective haze mitigation is achievable by intervening in the sulfate formation process with enforced NH3 and NO2 control measures. In addition to explaining the polluted episodes currently occurring in China and during the 1952 London Fog, this sulfate production mechanism is widespread, and our results suggest a way to tackle this growing problem in China and much of the developing world. PMID:27849598

[Inhibition of chlorobenzene formation via various routes during waste incineration by ammonium sulfate and urea].

PubMed

Yan, Mi; Qi, Zhi-Fu; Li, Xiao-Dong; Hu, Yan-Jun; Chen, Tong

2014-01-01

Chlorobenzene (CBz) is the precursor of polychlorinated dibenzo-p-dioxins/polychlorinated dibenzofurans (PCDD/Fs) generated in the processes of waste incineration, and it is regarded as a good indicator of PCDD/Fs for realizing PCDD/Fs online monitoring, moreover, pentachlorobenzene (PeCBz) and Hexachlorobenzene (HxCBz) belong to Persistent Organic Pollutants (POPs). However, the emission control of CBz in waste incineration does not attract enough attention, so this study focused on the inhibition of the 3 CBz formation routes in waste combustion by ammonium sulfate and urea, including CB formation from fly ash, CB formation from 1,2-dichlorobenzene (1,2-DiCBz) and the combustion of model medical waste. The results showed that both ammonium sulfate and urea reduced CBz yield during these three thermal processes. For instance, the inhibition rates of tetrachlorobenzene (TeCBz), PeCBz and HxCBz were 66.8%, 57.4% and 50.4%, respectively, when 1% urea was co-combusted with medical waste. By comparing the effect of ammonium sulfate and urea on CBz formation by three routes, urea was considered as a comparatively stable inhibitor for CBz.

Information persistence using XML database technology

NASA Astrophysics Data System (ADS)

Clark, Thomas A.; Lipa, Brian E. G.; Macera, Anthony R.; Staskevich, Gennady R.

2005-05-01

The Joint Battlespace Infosphere (JBI) Information Management (IM) services provide information exchange and persistence capabilities that support tailored, dynamic, and timely access to required information, enabling near real-time planning, control, and execution for DoD decision making. JBI IM services will be built on a substrate of network centric core enterprise services and when transitioned, will establish an interoperable information space that aggregates, integrates, fuses, and intelligently disseminates relevant information to support effective warfighter business processes. This virtual information space provides individual users with information tailored to their specific functional responsibilities and provides a highly tailored repository of, or access to, information that is designed to support a specific Community of Interest (COI), geographic area or mission. Critical to effective operation of JBI IM services is the implementation of repositories, where data, represented as information, is represented and persisted for quick and easy retrieval. This paper will address information representation, persistence and retrieval using existing database technologies to manage structured data in Extensible Markup Language (XML) format as well as unstructured data in an IM services-oriented environment. Three basic categories of database technologies will be compared and contrasted: Relational, XML-Enabled, and Native XML. These technologies have diverse properties such as maturity, performance, query language specifications, indexing, and retrieval methods. We will describe our application of these evolving technologies within the context of a JBI Reference Implementation (RI) by providing some hopefully insightful anecdotes and lessons learned along the way. This paper will also outline future directions, promising technologies and emerging COTS products that can offer more powerful information management representations, better persistence mechanisms and improved retrieval techniques.

Practitioner review: Effective ingredients of prevention programs for youth at risk of persistent juvenile delinquency--recommendations for clinical practice.

PubMed

de Vries, Sanne L A; Hoeve, Machteld; Assink, Mark; Stams, Geert Jan J M; Asscher, Jessica J

2015-02-01

There is a lack of knowledge about specific effective ingredients of prevention programs for youth at risk for persistent delinquent behavior. The present study combines findings of previous studies by examining the effectiveness of programs in preventing persistent juvenile delinquency and by studying which particular program, sample, and study characteristics contribute to the effects. Information on effective ingredients offers specific indications of how programs may be improved in clinical practice. A literature search in PsychINFO, ERIC, PubMed, Sociological Abstracts, Criminal Justice Abstracts, and Google Scholar was performed. Only (quasi)experimental studies and studies that focused on adolescents at risk for (persistent) delinquent behavior were included. Multilevel meta-analysis was conducted on 39 studies (N = 9,084). Participants' ages ranged from 6 to 20 years (M = 14 years, SD = 2.45). The overall effect size was significant and small in magnitude (d = 0.24, p < .001). Behavioral-oriented programs, focusing on parenting skills training, behavioral modeling, or behavioral contracting yielded the largest effects. Multimodal programs and programs carried out in the family context proved to be more beneficial than individual and group-based programs. Less intensive programs yielded larger effects. Prevention programs have positive effects on preventing persistent juvenile delinquency. In order to improve program effectiveness, interventions should be behavioral-oriented, delivered in a family or multimodal format, and the intensity of the program should be matched to the level of risk of the juvenile. © 2014 Association for Child and Adolescent Mental Health.

Increased persistence via asynchrony in oscillating ecological populations with long-range interaction

NASA Astrophysics Data System (ADS)

Gupta, Anubhav; Banerjee, Tanmoy; Dutta, Partha Sharathi

2017-10-01

Understanding the influence of the structure of a dispersal network on the species persistence and modeling a realistic species dispersal in nature are two central issues in spatial ecology. A realistic dispersal structure which favors the persistence of interacting ecological systems was studied [M. D. Holland and A. Hastings, Nature (London) 456, 792 (2008), 10.1038/nature07395], where it was shown that a randomization of the structure of a dispersal network in a metapopulation model of prey and predator increases the species persistence via clustering, prolonged transient dynamics, and amplitudes of population fluctuations. In this paper, by contrast, we show that a deterministic network topology in a metapopulation can also favor asynchrony and prolonged transient dynamics if species dispersal obeys a long-range interaction governed by a distance-dependent power law. To explore the effects of power-law coupling, we take a realistic ecological model, namely, the Rosenzweig-MacArthur model in each patch (node) of the network of oscillators, and show that the coupled system is driven from synchrony to asynchrony with an increase in the power-law exponent. Moreover, to understand the relationship between species persistence and variations in power-law exponent, we compute a correlation coefficient to characterize cluster formation, a synchrony order parameter, and median predator amplitude. We further show that smaller metapopulations with fewer patches are more vulnerable to extinction as compared to larger metapopulations with a higher number of patches. We believe that the present work improves our understanding of the interconnection between the random network and the deterministic network in theoretical ecology.

The Regulation of Transcription in Memory Consolidation

PubMed Central

Alberini, Cristina M.; Kandel, Eric R.

2015-01-01

De novo transcription of DNA is a fundamental requirement for the formation of long-term memory. It is required during both consolidation and reconsolidation, the posttraining and postreactivation phases that change the state of the memory from a fragile into a stable and long-lasting form. Transcription generates both mRNAs that are translated into proteins, which are necessary for the growth of new synaptic connections, as well as noncoding RNA transcripts that have regulatory or effector roles in gene expression. The result is a cascade of events that ultimately leads to structural changes in the neurons that mediate long-term memory storage. The de novo transcription, critical for synaptic plasticity and memory formation, is orchestrated by chromatin and epigenetic modifications. The complexity of transcription regulation, its temporal progression, and the effectors produced all contribute to the flexibility and persistence of long-term memory formation. In this article, we provide an overview of the mechanisms contributing to this transcriptional regulation underlying long-term memory formation. PMID:25475090

On the persistence of reproductive barriers in Eucalyptus: the bridging of mechanical barriers to zygote formation by F1 hybrids is counteracted by intrinsic post-zygotic incompatibilities.

PubMed

Larcombe, Matthew J; Costa E Silva, João; Tilyard, Paul; Gore, Peter; Potts, Brad M

2016-09-01

Many previous studies conclude that pre-zygotic barriers such as mechanical isolation account for most reproductive isolation between pairs of taxa. However, the inheritance and persistence of barriers such as these after the first generation of hybridization is rarely quantified, even though it is a vital consideration in understanding gene flow potential. There is an asymmetrical pre-zygotic mechanical barrier to hybridization between Eucalyptus nitens and Eucalyptus globulus, which completely prevents small-flowered E. nitens pollen from mating with large E. globulus flowers, while the reverse cross is possible. We aimed to determine the relative importance of pre- and post-zygotic barriers in preventing gene flow following secondary contact between E. nitens and E. globulus, including the inheritance of barriers in advanced-generation hybrids. Experimental crossing was used to produce outcrossed E. nitens, E. globulus and their F1, F2, BCg and BCn hybrids. The strength and inheritance of a suite of pre- and post-zygotic barriers were assessed, including 20-year survival, growth and reproductive capacity. The mechanical barrier to hybridization was lost or greatly reduced in the F1 hybrid. In contrast, intrinsic post-zygotic barriers were strong and persistent. Line-cross analysis indicated that the outbreeding depression in the hybrids was best explained by epistatic loss. The removal of strong mechanical barriers between E. nitens and E. globulus allows F1 hybrids to act as a bridge for bi-directional gene flow between these species. However, strong and persistent post-zygotic barriers exist, meaning that wherever F1 hybridization does occur, intrinsic post-zygotic barriers will be responsible for most reproductive isolation in this system. This potential transient nature of mechanical barriers to zygote formation due to additive inheritance in hybrids appears under-appreciated, and highlights the often important role that intrinsic post-mating barriers play in maintaining species boundaries at zones of secondary contact. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Vinculin is required for cell polarization, migration, and extracellular matrix remodeling in 3D collagen.

PubMed

Thievessen, Ingo; Fakhri, Nikta; Steinwachs, Julian; Kraus, Viola; McIsaac, R Scott; Gao, Liang; Chen, Bi-Chang; Baird, Michelle A; Davidson, Michael W; Betzig, Eric; Oldenbourg, Rudolf; Waterman, Clare M; Fabry, Ben

2015-11-01

Vinculin is filamentous (F)-actin-binding protein enriched in integrin-based adhesions to the extracellular matrix (ECM). Whereas studies in 2-dimensional (2D) tissue culture models have suggested that vinculin negatively regulates cell migration by promoting cytoskeleton-ECM coupling to strengthen and stabilize adhesions, its role in regulating cell migration in more physiologic, 3-dimensional (3D) environments is unclear. To address the role of vinculin in 3D cell migration, we analyzed the morphodynamics, migration, and ECM remodeling of primary murine embryonic fibroblasts (MEFs) with cre/loxP-mediated vinculin gene disruption in 3D collagen I cultures. We found that vinculin promoted 3D cell migration by increasing directional persistence. Vinculin was necessary for persistent cell protrusion, cell elongation, and stable cell orientation in 3D collagen, but was dispensable for lamellipodia formation, suggesting that vinculin-mediated cell adhesion to the ECM is needed to convert actin-based cell protrusion into persistent cell shape change and migration. Consistent with this finding, vinculin was necessary for efficient traction force generation in 3D collagen without affecting myosin II activity and promoted 3D collagen fiber alignment and macroscopical gel contraction. Our results suggest that vinculin promotes directionally persistent cell migration and tension-dependent ECM remodeling in complex 3D environments by increasing cell-ECM adhesion and traction force generation. © FASEB.

Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding

PubMed Central

De Andrade, Paula B. M.; Neff, Laurence A.; Strosova, Miriam K.; Arsenijevic, Denis; Patthey-Vuadens, Ophélie; Scapozza, Leonardo; Montani, Jean-Pierre; Ruegg, Urs T.; Dulloo, Abdul G.; Dorchies, Olivier M.

2015-01-01

Weight regain after caloric restriction results in accelerated fat storage in adipose tissue. This catch-up fat phenomenon is postulated to result partly from suppressed skeletal muscle thermogenesis, but the underlying mechanisms are elusive. We investigated whether the reduced rate of skeletal muscle contraction-relaxation cycle that occurs after caloric restriction persists during weight recovery and could contribute to catch-up fat. Using a rat model of semistarvation-refeeding, in which fat recovery is driven by suppressed thermogenesis, we show that contraction and relaxation of leg muscles are slower after both semistarvation and refeeding. These effects are associated with (i) higher expression of muscle deiodinase type 3 (DIO3), which inactivates tri-iodothyronine (T3), and lower expression of T3-activating enzyme, deiodinase type 2 (DIO2), (ii) slower net formation of T3 from its T4 precursor in muscles, and (iii) accumulation of slow fibers at the expense of fast fibers. These semistarvation-induced changes persisted during recovery and correlated with impaired expression of transcription factors involved in slow-twitch muscle development. We conclude that diminished muscle thermogenesis following caloric restriction results from reduced muscle T3 levels, alteration in muscle-specific transcription factors, and fast-to-slow fiber shift causing slower contractility. These energy-sparing effects persist during weight recovery and contribute to catch-up fat. PMID:26441673

Haemophilus parainfluenzae Strain ATCC 33392 Forms Biofilms In Vitro and during Experimental Otitis Media Infections

PubMed Central

Pang, Bing

2017-01-01

ABSTRACT Haemophilus parainfluenzae is a nutritionally fastidious, Gram-negative bacterium with an oropharyngeal/nasopharyngeal carriage niche that is associated with a range of opportunistic infections, including infectious endocarditis and otitis media (OM). These infections are often chronic/recurrent in nature and typically involve bacterial persistence within biofilm communities that are highly resistant to host clearance. This study addresses the primary hypothesis that H. parainfluenzae forms biofilm communities that are important determinants of persistence in vivo. The results from in vitro biofilm studies confirmed that H. parainfluenzae formed biofilm communities within which the polymeric matrix was mainly composed of extracellular DNA and proteins. Using a chinchilla OM infection model, we demonstrated that H. parainfluenzae formed surface-associated biofilm communities containing bacterial and host components that included neutrophil extracellular trap (NET) structures and that the bacteria mainly persisted in these biofilm communities. We also used this model to examine the possible interaction between H. parainfluenzae and its close relative Haemophilus influenzae, which is also commonly carried within the same host environments and can cause OM. The results showed that coinfection with H. influenzae promoted clearance of H. parainfluenzae from biofilm communities during OM infection. The underlying mechanisms for bacterial persistence and biofilm formation by H. parainfluenzae and knowledge about the survival defects of H. parainfluenzae during coinfection with H. influenzae are topics for future work. PMID:28674033

Haemophilus parainfluenzae Strain ATCC 33392 Forms Biofilms In Vitro and during Experimental Otitis Media Infections.

PubMed

Pang, Bing; Swords, W Edward

2017-09-01

Haemophilus parainfluenzae is a nutritionally fastidious, Gram-negative bacterium with an oropharyngeal/nasopharyngeal carriage niche that is associated with a range of opportunistic infections, including infectious endocarditis and otitis media (OM). These infections are often chronic/recurrent in nature and typically involve bacterial persistence within biofilm communities that are highly resistant to host clearance. This study addresses the primary hypothesis that H. parainfluenzae forms biofilm communities that are important determinants of persistence in vivo The results from in vitro biofilm studies confirmed that H. parainfluenzae formed biofilm communities within which the polymeric matrix was mainly composed of extracellular DNA and proteins. Using a chinchilla OM infection model, we demonstrated that H. parainfluenzae formed surface-associated biofilm communities containing bacterial and host components that included neutrophil extracellular trap (NET) structures and that the bacteria mainly persisted in these biofilm communities. We also used this model to examine the possible interaction between H. parainfluenzae and its close relative Haemophilus influenzae , which is also commonly carried within the same host environments and can cause OM. The results showed that coinfection with H. influenzae promoted clearance of H. parainfluenzae from biofilm communities during OM infection. The underlying mechanisms for bacterial persistence and biofilm formation by H. parainfluenzae and knowledge about the survival defects of H. parainfluenzae during coinfection with H. influenzae are topics for future work. Copyright © 2017 American Society for Microbiology.

Surface Measurements of Solar Spectral Radiative Flux in the Cloud-Free Atmosphere

NASA Technical Reports Server (NTRS)

Pilewskie, Peter; Goetz, A. F. H.; Bergstrom, R.; Beal, D.; Gore, Warren J. Y. (Technical Monitor)

1997-01-01

Recent studies (Charlock, et al.; Kato, et. al) have indicated a potential discrepancy between measured solar irradiance in the cloud-free atmosphere and model derived downwelling solar irradiance. These conclusions were based primarily on broadband integrated solar flux. Extinction (both absorption and scattering) phenomena, however, typically have spectral characteristics that would be present in moderate resolution (e.g., 10 nm) spectra, indicating the need for such measurements to thoroughly investigate the cause of any discrepancies. The 1996 Department of Energy Atmospheric Radiation Measurement Program (ARM) Intensive Observation Period (IOP), held simultaneously with the NASA Subsonic Aircraft: Contrail and Cloud Effects Special Study (SUCCESS) Program, provided an opportunity for two simultaneous but independent measurements of moderate resolution solar spectral downwelling irradiance at the surface. The instruments were the NASA Ames Solar Spectral Flux Radiometer and the Analytical Spectral Devices, Inc., FieldSpecT-FR. Spectral and band integrated quantities from both sets of measurements will be presented, along with estimates of the downwelling solar irradiance from band model and line by line calculations, in an effort to determine the compatibility between measured and calculated solar irradiance in the cloud-free atmosphere.

STS-96 Discovery night landing front view

NASA Technical Reports Server (NTRS)

1999-01-01

Bright lights at KSC's Shuttle Landing Facility runway 15 illuminate the landing of Space Shuttle Discovery, which completes the 9-day, 19-hour, 13-minute and 1-second long STS-96 mission. A contrail streams from the wing. Main gear touchdown was at 2:02:43 EDT June 6 , landing on orbit 154 of the mission. Nose gear touchdown was at 2:02:59 a.m. EDT, and the wheels stopped at 2:03:39 a.m. EDT. At the controls were Commander Kent V. Rominger and Pilot Rick D. Husband. Also onboard the orbiter were Mission Specialists Ellen Ochoa (Ph.D.), Tamara E. Jernigan (Ph.D.), Daniel S. Barry (M.D., Ph.D.), Julie Payette and Valery Ivanovich Tokarev. Payette represents the Canadian Space Agency and Tokarev the Russian Space Agency. The crew returned from the second flight to the International Space Station on a logistics and resupply mission. This was the 94th flight in the Space Shuttle program and the 26th for Discovery, also marking the 47th at KSC, the 24th in the last 25 missions, 11th at night, and the 18th consecutive landing in Florida.

Mesoscale Variability in SUCCESS Data

NASA Technical Reports Server (NTRS)

Eckermann, Stephen D.; Stewart, Richard W. (Technical Monitor)

1998-01-01

Analysis of meteorological, chemical and microphysical data from the airborne SUCCESS (SUbsonic aircraft Contrail and Cloud Effects Special Study) mission is reported. Careful analysis of the complex DC-8 flight pattern of May 2, 1996 reveals 19 linear flight segments within six main geographical areas, which we have analyzed. Significant mountain wave activity is revealed in the data from the MMS (Meteorology Measurement System) and MTP (Microwave Temperature Profiler) instruments on the DC-8, which resembles previous observations of mountain wave structures near Boulder, Colorado. Strong mountain-wave-induced upwelling downwind of the Rockies is noted. Turbulence is also noted in regions of the mountain wave consistent with overturning near the tropopause. Zonal winds recorded on the ER-2 are shown to be consistent with mountain wave breaking at or near critical levels in the stratosphere, consistent with the strong turbulence reported by the pilot during the ER-2 flight. These observations have been supported with spectral analyses and modeling studies. 'Postcasts' of mountain wave activity on May 2, 1996 using the Naval Research Laboratory Mountain Wave Forecast Model predicts both strong mountain wave activity near the tropopause and strong mountain-wave-induced turbulence in the stratosphere.

KSC-2011-4189

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Endeavour as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. On board are STS-134 Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialists Mike Fincke, Drew Feustel, Greg Chamitoff and the European Space Agency's Roberto Vittori. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kevin O'Connell

KSC-2011-4172

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- A vapor trail follows space shuttle Endeavour as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. On board are STS-134 Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialists Mike Fincke, Drew Feustel, Greg Chamitoff and the European Space Agency's Roberto Vittori. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Chuck Tintera

KSC-2011-4190

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- Vapor trails follow space shuttle Endeavour as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. On board are STS-134 Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialists Mike Fincke, Drew Feustel, Greg Chamitoff and the European Space Agency's Roberto Vittori. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Kevin O'Connell

KSC-2011-4186

NASA Image and Video Library

2011-06-01

CAPE CANAVERAL, Fla. -- A vapor trail follows space shuttle Endeavour as it approaches Runway 15 on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida for the final time. A vapor trail, known as a contrail, is a cloud of water vapor that condenses and freezes around the small particles in aircraft exhaust. Main gear touchdown was at 2:34:51 a.m. EDT, followed by nose gear touchdown at 2:35:04 a.m., and wheelstop at 2:35:36 a.m. On board are STS-134 Commander Mark Kelly, Pilot Greg H. Johnson, and Mission Specialists Mike Fincke, Drew Feustel, Greg Chamitoff and the European Space Agency's Roberto Vittori. STS-134 delivered the Alpha Magnetic Spectrometer-2 (AMS) and the Express Logistics Carrier-3 (ELC-3) to the International Space Station. AMS will help researchers understand the origin of the universe and search for evidence of dark matter, strange matter and antimatter from the station. ELC-3 carried spare parts that will sustain station operations once the shuttles are retired from service. STS-134 was the 25th and final flight for Endeavour, which has spent 299 days in space, orbited Earth 4,671 times and traveled 122,883,151 miles. Photo credit: NASA/Tom Joseph

STS-28 Columbia - Orbiter Vehicle (OV)-102 - Crew Insignia

NASA Image and Video Library

1988-10-11

S88-40309 (7 Nov. 1988) --- The STS-28 insignia was designed by the astronaut crew, who said it portrays the pride the American people have in their manned spaceflight program. It depicts America (the eagle) guiding the space program (the space shuttle) safely home from an orbital mission. The view looks south on Baja California and the west coast of the United States as the space travelers re-enter the atmosphere. The hypersonic contrails created by the eagle and shuttle represent the American flag. The crew called the simple boldness of the design symbolic of America's unfaltering commitment to leadership in the exploration and development of space. Crew members for STS-28 are astronauts Brewster H. Shaw Jr.,commander; Richard N. Richards, pilot; and David C. Leestma, Mark N. Brown and James C. Adamson, mission specialists. The NASA insignia design for space shuttle flights is reserved for use by the astronauts and for other official use as the NASA Administrator may authorize. Public availability has been approved only in the forms of illustrations by the various news media. When and if there is any change in this policy, which is not anticipated, the change will be publicly announced. Photo credit: NASA

Modelling exhaust plume mixing in the near field of an aircraft

NASA Astrophysics Data System (ADS)

Garnier, F.; Brunet, S.; Jacquin, L.

1997-11-01

A simplified approach has been applied to analyse the mixing and entrainment processes of the engine exhaust through their interaction with the vortex wake of an aircraft. Our investigation is focused on the near field, extending from the exit nozzle until about 30 s after the wake is generated, in the vortex phase. This study was performed by using an integral model and a numerical simulation for two large civil aircraft: a two-engine Airbus 330 and a four-engine Boeing 747. The influence of the wing-tip vortices on the dilution ratio (defined as a tracer concentration) shown. The mixing process is also affected by the buoyancy effect, but only after the jet regime, when the trapping in the vortex core has occurred. In the early wake, the engine jet location (i.e. inboard or outboard engine jet) has an important influence on the mixing rate. The plume streamlines inside the vortices are subject to distortion and stretching, and the role of the descent of the vortices on the maximum tracer concentration is discussed. Qualitative comparison with contrail photograph shows similar features. Finally, tracer concentration of inboard engine centreline of B-747 are compared with other theoretical analyses and measured data.

Quantifying expert consensus against the existence of a secret, large-scale atmospheric spraying program

NASA Astrophysics Data System (ADS)

Shearer, Christine; West, Mick; Caldeira, Ken; Davis, Steven J.

2016-08-01

Nearly 17% of people in an international survey said they believed the existence of a secret large-scale atmospheric program (SLAP) to be true or partly true. SLAP is commonly referred to as ‘chemtrails’ or ‘covert geoengineering’, and has led to a number of websites purported to show evidence of widespread chemical spraying linked to negative impacts on human health and the environment. To address these claims, we surveyed two groups of experts—atmospheric chemists with expertize in condensation trails and geochemists working on atmospheric deposition of dust and pollution—to scientifically evaluate for the first time the claims of SLAP theorists. Results show that 76 of the 77 scientists (98.7%) that took part in this study said they had not encountered evidence of a SLAP, and that the data cited as evidence could be explained through other factors, including well-understood physics and chemistry associated with aircraft contrails and atmospheric aerosols. Our goal is not to sway those already convinced that there is a secret, large-scale spraying program—who often reject counter-evidence as further proof of their theories—but rather to establish a source of objective science that can inform public discourse.

Patterning the dorsal longitudinal flight muscles (DLM) of Drosophila: insights from the ablation of larval scaffolds

NASA Technical Reports Server (NTRS)

Fernandes, J. J.; Keshishian, H.

1996-01-01

The six Dorsal Longitudinal flight Muscles (DLMs) of Drosophila develop from three larval muscles that persist into metamorphosis and serve as scaffolds for the formation of the adult fibers. We have examined the effect of muscle scaffold ablation on the development of DLMs during metamorphosis. Using markers that are specific to muscle and myoblasts we show that in response to the ablation, myoblasts which would normally fuse with the larval muscle, fuse with each other instead, to generate the adult fibers in the appropriate regions of the thorax. The development of these de novo DLMs is delayed and is reflected in the delayed expression of erect wing, a transcription factor thought to control differentiation events associated with myoblast fusion. The newly arising muscles express the appropriate adult-specific Actin isoform (88F), indicating that they have the correct muscle identity. However, there are frequent errors in the number of muscle fibers generated. Ablation of the larval scaffolds for the DLMs has revealed an underlying potential of the DLM myoblasts to initiate de novo myogenesis in a manner that resembles the mode of formation of the Dorso-Ventral Muscles, DVMs, which are the other group of indirect flight muscles. Therefore, it appears that the use of larval scaffolds is a superimposition on a commonly used mechanism of myogenesis in Drosophila. Our results show that the role of the persistent larval muscles in muscle patterning involves the partitioning of DLM myoblasts, and in doing so, they regulate formation of the correct number of DLM fibers.

Dact1-3 mRNAs exhibit distinct expression domains during tooth development

PubMed Central

Kettunen, Päivi; Kivimäe, Saul; Keshari, Pankaj; Klein, Ophir D.; Cheyette, Benjamin N.R.; Luukko, Keijo

2010-01-01

Wnt signaling is essential for tooth formation. Dact proteins modulate Wnt signaling by binding to the intracellular protein Dishevelled (Dvl). Comparison of all known mouse Dact genes, Dact1-3, from the morphological initiation of mandibular first molar development after the onset of the root formation using sectional in situ hybridization showed distinct, complementary and overlapping expression patterns for the studied genes. While Dact2 expression was restricted to the dental epithelium including the enamel knot signaling centers and tooth specific preameloblasts, Dact1 and Dact3 showed developmentally regulated expression in the dental mesenchyme. Both mRNAs were first detected in the presumptive dental mesenchyme. After being downregulated from the condensed dental mesenchyme of the bud stage tooth germ, Dact1 was upregulated in the dental follicle masenchyme at the cap stage and subsequently also in the dental papilla at the bell stage where the expression persisted to the postnatal stages. In contrast, Dact3 transcripts persisted throughout the dental mesenchymal tissue components including the tooth-specific cells, preodontoblasts before transcripts were largely downregulated from the tooth germ postnatally. Collectively these results suggest that Dact1 and -3 may contribute to early tooth formation by modulation of Wnt signaling pathways in the mesenchyme, including preodontoblasts, whereas Dact2 may play important signal-modulating roles in the adjacent epithelial cells including the enamel knot signaling centers and preameloblasts. Future loss-of-function studies will help elucidate whether any of these functions are redundant, particularly for Dact1 and Dact3. PMID:20170752

Helicobacter pylori Biofilm Formation and Its Potential Role in Pathogenesis.

PubMed

Hathroubi, Skander; Servetas, Stephanie L; Windham, Ian; Merrell, D Scott; Ottemann, Karen M

2018-06-01

Despite decades of effort, Helicobacter pylori infections remain difficult to treat. Over half of the world's population is infected by H. pylori , which is a major cause of duodenal and gastric ulcers as well as gastric cancer. During chronic infection, H. pylori localizes within the gastric mucosal layer, including deep within invaginations called glands; thanks to its impressive ability to survive despite the harsh acidic environment, it can persist for the host's lifetime. This ability to survive and persist in the stomach is associated with urease production, chemotactic motility, and the ability to adapt to the fluctuating environment. Additionally, biofilm formation has recently been suggested to play a role in colonization. Biofilms are surface-associated communities of bacteria that are embedded in a hydrated matrix of extracellular polymeric substances. Biofilms pose a substantial health risk and are key contributors to many chronic and recurrent infections. This link between biofilm-associated bacteria and chronic infections likely results from an increased tolerance to conventional antibiotic treatments as well as immune system action. The role of this biofilm mode in antimicrobial treatment failure and H. pylori survival has yet to be determined. Furthermore, relatively little is known about the H. pylori biofilm structure or the genes associated with this mode of growth. In this review, therefore, we aim to highlight recent findings concerning H. pylori biofilms and the molecular mechanism of their formation. Additionally, we discuss the potential roles of biofilms in the failure of antibiotic treatment and in infection recurrence. Copyright © 2018 American Society for Microbiology.

Condensin II Resolves Chromosomal Associations to Enable Anaphase I Segregation in Drosophila Male Meiosis

PubMed Central

Hartl, Tom A.; Sweeney, Sarah J.; Knepler, Peter J.; Bosco, Giovanni

2008-01-01

Several meiotic processes ensure faithful chromosome segregation to create haploid gametes. Errors to any one of these processes can lead to zygotic aneuploidy with the potential for developmental abnormalities. During prophase I of Drosophila male meiosis, each bivalent condenses and becomes sequestered into discrete chromosome territories. Here, we demonstrate that two predicted condensin II subunits, Cap-H2 and Cap-D3, are required to promote territory formation. In mutants of either subunit, territory formation fails and chromatin is dispersed throughout the nucleus. Anaphase I is also abnormal in Cap-H2 mutants as chromatin bridges are found between segregating heterologous and homologous chromosomes. Aneuploid sperm may be generated from these defects as they occur at an elevated frequency and are genotypically consistent with anaphase I segregation defects. We propose that condensin II–mediated prophase I territory formation prevents and/or resolves heterologous chromosomal associations to alleviate their potential interference in anaphase I segregation. Furthermore, condensin II–catalyzed prophase I chromosome condensation may be necessary to resolve associations between paired homologous chromosomes of each bivalent. These persistent chromosome associations likely consist of DNA entanglements, but may be more specific as anaphase I bridging was rescued by mutations in the homolog conjunction factor teflon. We propose that the consequence of condensin II mutations is a failure to resolve heterologous and homologous associations mediated by entangled DNA and/or homolog conjunction factors. Furthermore, persistence of homologous and heterologous interchromosomal associations lead to anaphase I chromatin bridging and the generation of aneuploid gametes. PMID:18927632

Formation of Environmentally Persistent Free Radical (EPFR) in Iron(III) Cation-Exchanged Smectite Clay

PubMed Central

Nwosu, Ugwumsinachi G.; Roy, Amitava; dela Cruz, Albert Leo N.; Dellinger, Barry; Cook, Robert

2016-01-01

Environmentally persistent free radicals (EPFRs) have been found at a number of Superfund sites, with EPFRs being formed via a proposed redox process at ambient environmental conditions. The possibility of such a redox process taking place at ambient environmental conditions is studied utilizing a surrogate soil system of phenol and iron(III)-exchanged calcium montmorillonite clay, Fe(III)CaM. Sorption of phenol by the Fe(III)CaM is demonstrated by Fourier-transformed infra-red (FT-IR) spectroscopy, as evidenced by the peaks between 1345 cm−1 and 1595 cm−1, and at lower frequencies between 694 cm−1 and 806 cm−1, as well as X-ray diffraction (XRD) spectroscopy, as shown by an increase in interlayer spacing within Fe(III)CaM. The formation and characterization of the EPFRs is determined by electron paramagnetic resonance (EPR) spectroscopy, showing phenoxyl-type radical with a g-factor of 2.0034 and ΔHp-p of 6.1 G at an average concentration of 7.5 × 1017 spins/g. EPFRs lifetime data are indicative of oxygen and water molecules being responsible for EPFR decay. The change in the oxidation state of the iron redox center is studied by X-ray absorption near-edge structure (XANES) spectroscopy, showing that 23% of the Fe(III) is reduced to Fe(II). X-ray photoemission spectroscopy (XPS) results confirm the XANES results. These findings, when combined with the EPFR concentration data, demonstrate that the stoichiometry of the EPFR formation under the conditions of this study is 1.5 × 10−2 spins/Fe(II) atom. PMID:26647158

Formation of environmentally persistent free radical (EPFR) in iron(III) cation-exchanged smectite clay.

PubMed

Nwosu, Ugwumsinachi G; Roy, Amitava; dela Cruz, Albert Leo N; Dellinger, Barry; Cook, Robert

2016-01-01

Environmentally persistent free radicals (EPFRs) have been found at a number of Superfund sites, with EPFRs being formed via a proposed redox process at ambient environmental conditions. The possibility of such a redox process taking place at ambient environmental conditions is studied utilizing a surrogate soil system of phenol and iron(III)-exchanged calcium montmorillonite clay, Fe(III)CaM. Sorption of phenol by the Fe(III)CaM is demonstrated by Fourier-transformed infra-red (FT-IR) spectroscopy, as evidenced by the peaks between 1345 cm(-1) and 1595 cm(-1), and at lower frequencies between 694 cm(-1) and 806 cm(-1), as well as X-ray diffraction (XRD) spectroscopy, as shown by an increase in interlayer spacing within Fe(III)CaM. The formation and characterization of the EPFRs is determined by electron paramagnetic resonance (EPR) spectroscopy, showing phenoxyl-type radical with a g-factor of 2.0034 and ΔHP-P of 6.1 G at an average concentration of 7.5 × 10(17) spins per g. EPFRs lifetime data are indicative of oxygen and water molecules being responsible for EPFR decay. The change in the oxidation state of the iron redox center is studied by X-ray absorption near-edge structure (XANES) spectroscopy, showing that 23% of the Fe(III) is reduced to Fe(II). X-ray photoemission spectroscopy (XPS) results confirm the XANES results. These findings, when combined with the EPFR concentration data, demonstrate that the stoichiometry of the EPFR formation under the conditions of this study is 1.5 × 10(-2) spins per Fe(II) atom.

Pendulum Exercises After Hip Arthroscopy: A Video Technique.

PubMed

Sauber, Ryan; Saborio, George; Nickel, Beth M; Kivlan, Benjamin R; Christoforetti, John J

2016-08-01

Advanced hip joint-preserving arthroscopic techniques have been shown to improve patient-reported functional outcomes with low rates of postoperative complications. Prior work has shown that formation of adhesive scar is a potential source of persistent pain and cause for revision surgery. As resources for postoperative in-studio physical therapy become scarce, a home-based strategy to avoid scar formation without adding formal therapy cost may be beneficial. The purpose of this technical note is to introduce a patient-centered educational video technique for home-caregiver delivery of manual hip pendulum exercises in the postoperative setting. This video technique offers access to our method for pendulum exercise as part of early recovery after advanced hip arthroscopy.

Ultrastructural blood-brain barrier alterations and edema formation in acute spinal cord trauma.

PubMed

Goodman, J H; Bingham, W G; Hunt, W E

1976-04-01

Endothelial changes leading to edema formation are examined in the primate spinal cord (Macaca mulatta) following a lesion created by a 20-gm weight falling 15 cm onto the exposed dura. Intravascular perfusion of a paraformaldehydeglutaraldehyde solution followed by carbon black provides adequate fixation of vascular structures and glial elements. Myelin is poorly preserved. Ultrastructural alterations of the blood-brain barrier consist of loss of integrity of the endothelial tight junctions. Edema caused by vascular disruption and parenchymatous extravasation of intravascular contents is observed along with glial swelling. Interglial gap junctions persist in areas of marked cellular seperation and do not impede the migration of edema fluid.

Bioconvection and front formation of Paramecium tetraurelia

NASA Astrophysics Data System (ADS)

Kitsunezaki, So; Komori, Rie; Harumoto, Terue

2007-10-01

We have investigated the bioconvection of Paramecium tetraurelia in high-density suspensions made by centrifugal concentration. When a suspension is kept at rest in a Hele-Shaw cell, a crowded front of paramecia is formed in the vicinity of the bottom and it propagates gradually toward the water-air interface. Fluid convection occurs under this front, and it is driven persistently by the upward swimming of paramecia. The roll structures of the bioconvection become turbulent with an increase in the depth of the suspension; they also change rapidly as the density of paramecia increases. Our experimental results suggest that lack of oxygen in the suspension causes the active individual motions of paramecia to induce the formation of this front.

The government of chronic poverty: from exclusion to citizenship?

PubMed

Hickey, Sam

2010-01-01

Development trustees have increasingly sought to challenge chronic poverty by promoting citizenship amongst poor people, a move that frames citizenship formation as central to overcoming the exclusions and inequalities associated with uneven development. For sceptics, this move within inclusive neoliberalism is inevitably depoliticising and disempowering, and our cases do suggest that citizenship-based strategies rarely alter the underlying basis of poverty. However, our evidence also offers some support to those optimists who suggest that progressive moves towards poverty reduction and citizenship formation have become more rather than less likely at the current juncture. The promotion of citizenship emerges here as a significant but incomplete effort to challenge poverty that persists over time.

Compressive response and helix formation of a semiflexible polymer confined in a nanochannel

NASA Astrophysics Data System (ADS)

Hayase, Yumino; Sakaue, Takahiro; Nakanishi, Hiizu

2017-05-01

Configurations of a single semiflexible polymer is studied when it is pushed into a nanochannel in the case where the polymer persistence length lp is much longer than the channel diameter D :lp/D ≫1 . Using numerical simulations, we show that the polymer undergoes a sequence of recurring structural transitions upon longitudinal compression: random deflection along the channel, a helix going around the channel wall, double-fold random deflection, double-fold helix, etc. We find that the helix transition can be understood as buckling of deflection segments, and the initial helix formation takes place at very small compression with no appreciable weak compression regime of the random deflection polymer.

Esherichia coli serotype O157:H7 retention on solid surfaces and peroxide resistance is enhanced by dual-strain biofilm formation

USDA-ARS?s Scientific Manuscript database

In a previous study we showed that an Escherichia coli O157:H7 strain that was unable to form biofilm could persist in large numbers in dual-strain biofilms formed with an E. coli O-:H4 companion strain. In this study we tested additional companion strains for their ability to retain serotype O157:H...