Super-rogue waves in simulations based on weakly nonlinear and fully nonlinear hydrodynamic equations.

PubMed

Slunyaev, A; Pelinovsky, E; Sergeeva, A; Chabchoub, A; Hoffmann, N; Onorato, M; Akhmediev, N

2013-07-01

The rogue wave solutions (rational multibreathers) of the nonlinear Schrödinger equation (NLS) are tested in numerical simulations of weakly nonlinear and fully nonlinear hydrodynamic equations. Only the lowest order solutions from 1 to 5 are considered. A higher accuracy of wave propagation in space is reached using the modified NLS equation, also known as the Dysthe equation. This numerical modeling allowed us to directly compare simulations with recent results of laboratory measurements in Chabchoub et al. [Phys. Rev. E 86, 056601 (2012)]. In order to achieve even higher physical accuracy, we employed fully nonlinear simulations of potential Euler equations. These simulations provided us with basic characteristics of long time evolution of rational solutions of the NLS equation in the case of near-breaking conditions. The analytic NLS solutions are found to describe the actual wave dynamics of steep waves reasonably well.

36 CFR 910.34 - Accommodations for the physically handicapped.

Code of Federal Regulations, 2014 CFR

2014-07-01

... physically handicapped. 910.34 Section 910.34 Parks, Forests, and Public Property PENNSYLVANIA AVENUE... § 910.34 Accommodations for the physically handicapped. (a) Every development shall incorporate features which will make the development accessible by the physically handicapped. The standards in the “American...

36 CFR 910.34 - Accommodations for the physically handicapped.

Code of Federal Regulations, 2012 CFR

2012-07-01

... physically handicapped. 910.34 Section 910.34 Parks, Forests, and Public Property PENNSYLVANIA AVENUE... § 910.34 Accommodations for the physically handicapped. (a) Every development shall incorporate features which will make the development accessible by the physically handicapped. The standards in the “American...

36 CFR § 910.34 - Accommodations for the physically handicapped.

Code of Federal Regulations, 2013 CFR

2013-07-01

... physically handicapped. § 910.34 Section § 910.34 Parks, Forests, and Public Property PENNSYLVANIA AVENUE... § 910.34 Accommodations for the physically handicapped. (a) Every development shall incorporate features which will make the development accessible by the physically handicapped. The standards in the “American...

Exploring the physics of the accretion and jet in nearby narrow-line Seyfert 1 galaxies

NASA Astrophysics Data System (ADS)

Yao, Su; Qiao, Erlin; Wu, Xue-Bing; You, B.

2018-06-01

In this paper, we explore the physics of the accretion and jet in narrow-line Seyfert 1 (NLS1) galaxy. Specifically, we compile a sample composed of 16 nearby NLS1 with Lbol/LEdd ≳ 0.1. We investigate the mutual correlation between their radio luminosity LR, X-ray luminosity LX, optical luminosity L5100, and black hole mass MBH. By adopting partial correlation analysis, we find (1) a positive correlation between LX and MBH and (2) a weak positive correlation between LR and L5100. However, we don't find significant correlations between LR and LX or between LX and L5100 after considering the effect of the black hole mass, which leads to a finding that LX/LEdd is independent of L5100/LEdd. Interestingly, the findings that LX is correlated with MBH and LX/LEdd is not correlated with L5100/LEdd support that the X-ray emission is saturated with increasing \\dot{M} for Lbol/LEdd ≳ 0.1 in NLS1, which may be understood in the framework of slim disc scenario. Finally, we suggest that a larger NLS1 sample with high-quality radio and X-ray data is needed to further confirm this result in the future.

Self-Esteem, Achievement Goals, and Self-Handicapping in College Physical Education.

PubMed

Chen, Zuosong; Sun, Kaihong; Wang, Kun

2017-01-01

This study aims to investigate the relationships among self-esteem, achievement goals, and self-handicapping and the potential mediating role of achievement goals in the relationship between self-esteem and self-handicapping in college physical education. The participants were 320 Chinese college students. Three validated scales were employed to assess participants' self-esteem, achievement goals, and self-handicapping in college physical education. Results showed that self-esteem had a negative effect on self-handicapping. Self-esteem had a positive effect on mastery goals, but had a negative effect on performance-avoidance goals. Mastery goals had a negative effect and performance-avoidance goals had a positive effect on self-handicapping. Moreover, mastery goals and performance-avoidance goals partially mediated the relationship between self-esteem and self-handicapping, and self-esteem had both direct and indirect effects on self-handicapping in college physical education. The findings indicate that improving individual's self-esteem and promoting mastery goals while reducing performance-avoidance goals may be relevant strategies to reduce self-handicapping in college physical education.

Assessing Student Teachers' Attitudes toward Mentally Retarded and Physically Handicapped Persons.

ERIC Educational Resources Information Center

Ashman, Adrian F.

1984-01-01

Analysis of questionnaires completed by 104 postgraduate level education students revealed that Ss were more accepting of physical than mental handicaps. No sex differences were found. Identified five factors: perceived rights and privileges of physically handicapped and retarded persons, interpersonal comfort with handicapped people, attitudes…

Determining the relation between quality of life, handicap, fitness, and physical activity for persons with spinal cord injury.

PubMed

Manns, P J; Chad, K E

1999-12-01

Determining relationships among fitness, physical activity, subjective quality of life, and handicap in persons with spinal cord injury (SCI). Cross-sectional exploratory study. University research laboratory setting. Twenty-eight men and 10 women (age 35.9+/-9.3 yrs) with SCI (17 quadriplegic, 21 paraplegic). Fitness (maximal incremental exercise test on arm ergometer), physical activity (leisure time exercise questionnaire), subjective quality of life (Quality of Life Profile: Physical and Sensory Disabilities Version), and level of handicap (Craig Handicap Assessment Reporting Technique). Correlational analyses examined relationships between the measures. Physical activity was correlated with composite handicap score in quadriplegic and paraplegic persons. Handicap domain scores for physical independence, mobility, and occupation were correlated with physical activity in quadriplegic persons. There was no relation between the subjective quality of life scores and fitness and physical activity in either group. Physical activity may play an important role in handicap for persons with SCI, particularly persons with quadriplegic injuries. The lack of a relation between activity variables and subjective quality of life may be related to the global nature of the measure used.

Solitonic dynamics and excitations of the nonlinear Schrödinger equation with third-order dispersion in non-Hermitian PT-symmetric potentials.

PubMed

Chen, Yong; Yan, Zhenya

2016-03-22

Solitons are of the important significant in many fields of nonlinear science such as nonlinear optics, Bose-Einstein condensates, plamas physics, biology, fluid mechanics, and etc. The stable solitons have been captured not only theoretically and experimentally in both linear and nonlinear Schrödinger (NLS) equations in the presence of non-Hermitian potentials since the concept of the parity-time -symmetry was introduced in 1998. In this paper, we present novel bright solitons of the NLS equation with third-order dispersion in some complex -symmetric potentials (e.g., physically relevant -symmetric Scarff-II-like and harmonic-Gaussian potentials). We find stable nonlinear modes even if the respective linear -symmetric phases are broken. Moreover, we also use the adiabatic changes of the control parameters to excite the initial modes related to exact solitons to reach stable nonlinear modes. The elastic interactions of two solitons are exhibited in the third-order NLS equation with -symmetric potentials. Our results predict the dynamical phenomena of soliton equations in the presence of third-order dispersion and -symmetric potentials arising in nonlinear fiber optics and other physically relevant fields.

Solitonic dynamics and excitations of the nonlinear Schrödinger equation with third-order dispersion in non-Hermitian PT-symmetric potentials

PubMed Central

Chen, Yong; Yan, Zhenya

2016-01-01

Solitons are of the important significant in many fields of nonlinear science such as nonlinear optics, Bose-Einstein condensates, plamas physics, biology, fluid mechanics, and etc. The stable solitons have been captured not only theoretically and experimentally in both linear and nonlinear Schrödinger (NLS) equations in the presence of non-Hermitian potentials since the concept of the parity-time -symmetry was introduced in 1998. In this paper, we present novel bright solitons of the NLS equation with third-order dispersion in some complex -symmetric potentials (e.g., physically relevant -symmetric Scarff-II-like and harmonic-Gaussian potentials). We find stable nonlinear modes even if the respective linear -symmetric phases are broken. Moreover, we also use the adiabatic changes of the control parameters to excite the initial modes related to exact solitons to reach stable nonlinear modes. The elastic interactions of two solitons are exhibited in the third-order NLS equation with -symmetric potentials. Our results predict the dynamical phenomena of soliton equations in the presence of third-order dispersion and -symmetric potentials arising in nonlinear fiber optics and other physically relevant fields. PMID:27002543

Making Facilities Accessible to the Physically Handicapped.

ERIC Educational Resources Information Center

State Univ. Construction Fund, Albany, NY.

Guidelines on performance criteria for the State University of New York consider two main types of handicapped: the ambulant and semi-ambulant, including some physically handicapped, the visually and aurally handicapped, and persons with cardiac conditions; and persons confined to wheel chairs. The handicapped and planning for them are discussed.…

24 CFR 235.331 - Increased maximum mortgage amount for physically handicapped persons.

Code of Federal Regulations, 2014 CFR

2014-04-01

... for physically handicapped persons. 235.331 Section 235.331 Housing and Urban Development Regulations... maximum mortgage amount for physically handicapped persons. If the mortgage relates to a dwelling unit to be occupied by a handicapped person as defined in § 235.5(c)(2), the otherwise applicable dollar...

24 CFR 235.331 - Increased maximum mortgage amount for physically handicapped persons.

Code of Federal Regulations, 2010 CFR

2010-04-01

... for physically handicapped persons. 235.331 Section 235.331 Housing and Urban Development Regulations... maximum mortgage amount for physically handicapped persons. If the mortgage relates to a dwelling unit to be occupied by a handicapped person as defined in § 235.5(c)(2), the otherwise applicable dollar...

24 CFR 235.331 - Increased maximum mortgage amount for physically handicapped persons.

Code of Federal Regulations, 2012 CFR

2012-04-01

... for physically handicapped persons. 235.331 Section 235.331 Housing and Urban Development Regulations... maximum mortgage amount for physically handicapped persons. If the mortgage relates to a dwelling unit to be occupied by a handicapped person as defined in § 235.5(c)(2), the otherwise applicable dollar...

24 CFR 235.331 - Increased maximum mortgage amount for physically handicapped persons.

Code of Federal Regulations, 2011 CFR

2011-04-01

... for physically handicapped persons. 235.331 Section 235.331 Housing and Urban Development Regulations... maximum mortgage amount for physically handicapped persons. If the mortgage relates to a dwelling unit to be occupied by a handicapped person as defined in § 235.5(c)(2), the otherwise applicable dollar...

24 CFR 235.331 - Increased maximum mortgage amount for physically handicapped persons.

Code of Federal Regulations, 2013 CFR

2013-04-01

... for physically handicapped persons. 235.331 Section 235.331 Housing and Urban Development Regulations... maximum mortgage amount for physically handicapped persons. If the mortgage relates to a dwelling unit to be occupied by a handicapped person as defined in § 235.5(c)(2), the otherwise applicable dollar...

The Narrow-Line Region of Narrow-Line Seyfert 1 Galaxies

NASA Astrophysics Data System (ADS)

Rodríguez-Ardila, A.; Binette, Luc; Pastoriza, Miriani G.; Donzelli, Carlos J.

2000-08-01

This work studies the optical emission-line properties and physical conditions of the narrow-line region (NLR) of seven narrow-line Seyfert 1 galaxies (NLS1's) for which high signal-to-noise ratio spectroscopic observations were available. The resolution is 340 km s-1 (at Hα) over the wavelength interval 3700-9500 Å, enabling us to separate the broad and narrow components of the permitted emission lines. Our results show that the flux carried out by the narrow component of Hβ is, on average, 50% of the total line flux. As a result, the [O III] λ5007/Hβ ratio emitted in the NLR varies from 1 to 5, instead of the universally adopted value of 10. This has strong implications for the required spectral energy distribution that ionizes the NLR gas. Photoionization models that consider a NLR composed of a combination of matter-bounded and ionization-bounded clouds are successful at explaining the low [O III] λ5007/Hβ ratio and the weakness of low-ionization lines of NLS1's. Variation of the relative proportion of these two type of clouds nicely reproduces the dispersion of narrow-line ratios found among the NLS1 sample. Assuming similar physical model parameters of both NLS1's and the normal Seyfert 1 galaxy NGC 5548, we show that the observed differences of emission-line ratios between these two groups of galaxies can be explained, to a first approximation, in terms of the shape of the input ionizing continuum. Narrow emission-line ratios of NLS1's are better reproduced by a steep power-law continuum in the EUV-soft X-ray region, with spectral index α~-2. Flatter spectral indices (α~-1.5) match the observed line ratios of NGC 5548 but are unable to provide a good match to the NLS1 ratios. This result is consistent with ROSAT observations of NLS1's, which show that these objects are characterized by steeper power-law indices than those of Seyfert 1 galaxies with strong broad optical lines. Based on observations made at CASLEO. Complejo Astronómico El Leoncito (CASLEO) is operated under agreement between the Consejo Nacional de Investigaciones Científicas y técnicas de la República Argentina and the National Universities of La Plata, Córdoba and San Juán.

46 CFR 153.1108 - Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 5 2014-10-01 2014-10-01 false Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity exceeds 25 mPa sec at 20 °C: Categories A, B, and C... Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity...

46 CFR 153.1108 - Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 5 2012-10-01 2012-10-01 false Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity exceeds 25 mPa sec at 20 °C: Categories A, B, and C... Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity...

46 CFR 153.1108 - Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 5 2013-10-01 2013-10-01 false Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity exceeds 25 mPa sec at 20 °C: Categories A, B, and C... Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity...

46 CFR 153.1108 - Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity exceeds 25 mPa sec at 20 °C: Categories A, B, and C... Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity...

46 CFR 153.1108 - Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity exceeds 25 mPa sec at 20 °C: Categories A, B, and C... Heated prewash for solidifying NLS, high viscosity NLS and required prewashes of NLS whose viscosity...

Trait self-esteem and claimed self-handicapping motives in sports situations.

PubMed

Finez, Lucie; Berjot, Sophie; Rosnet, Elisabeth; Cleveland, Christena; Tice, Dianne M

2012-12-01

We examined the relationship between physical self-esteem and claimed self-handicapping among athletes by taking motives into consideration. In Study 1, 99 athletes were asked to report their tendency to engage in claimed self-handicapping for self-protective and self-enhancement motives (trait measures). Low self-esteem athletes reported a higher tendency to engage in claimed self-handicapping for these two motives compared with high self-esteem athletes. Neither low nor high self-esteem athletes reported a preference for one motive over the other. In Study 2, 107 athletes participated in a test that was ostensibly designed to assess high physical abilities - and thus to encourage self-handicapping for self-enhancement motives (success-meaningful condition) - or to assess low physical abilities, and thus to encourage self-handicapping for self-protective motives (failure-meaningful condition). Before starting the test, athletes were given the opportunity to claim handicaps that could impair their performance. Low self-esteem athletes claimed more handicaps than high self-esteem athletes in both conditions. Findings suggest that low physical self-esteem athletes engage more in claimed handicapping regardless of motives, relative to high physical self-esteem athletes.

Primary Handicaps of Students in Programs for the Physically Handicapped.

ERIC Educational Resources Information Center

Hinesley, J. Howard, Comp.

Reported is a survey of types of handicaps served by special programs for physically handicapped, homebound, and hospitalized children in Florida. The information represents 215 full time and hourly teachers. Types of handicaps served are listed, ranked, and number of cases given. Cerebral palsy is reported to be the most common type of handicap…

The Design of a Computer Table for the Physically Handicapped Student.

ERIC Educational Resources Information Center

Fitterman, L. Jeffrey

The paper describes the development of a computer table for physically handicapped students including persons with moderate to severe cerebral palsy, muscular dystrophy, uncontrolled epilepsy, and paralysis due to physical trauma. The project first reviewed furniture currently available for the physically handicapped and then conducted ergonomic…

Fear of failure and self-handicapping in college physical education.

PubMed

Chen, Lung Hung; Chen, Mei-Yen; Lin, Meng-Shyan; Kee, Ying Hwa; Shui, Shang-Hsueh

2009-12-01

The purpose of this study was to examine the relationship between fear of failure and self-handicapping within the context of physical education. Participants were 103 college freshmen enrolled in aerobic dance physical education classes in Taiwan. They completed the Performance Failure Appraisal Inventory and Self-Handicapping Scale for Sport 3 mo. after entering the class. Hierarchical regression indicated that scores on fear of failure predicted self-handicapping scores.

Telecommunication for the Physically Handicapped.

ERIC Educational Resources Information Center

Cunningham, Pat; Gose, Joan

The paper examines the uses of telecommunication for physically handicapped students. Basic equipment, including a modem and keyboard interface, are described. The types and uses of computer bulletin boards are also described. Among benefits of telecommunications for physically handicapped students noted in the paper are social prestige,…

46 CFR 153.2 - Definitions and acronyms.

Code of Federal Regulations, 2013 CFR

2013-10-01

... viscosity NLS includes high viscosity Category B NLS and high viscosity Category C NLS. High viscosity Category B NLS means any Category B NLS having a viscosity of at least 25 mPa.s at 20 °C and at least 25 mPa.s at the time it is unloaded. High viscosity Category C NLS means any Category C NLS having a...

46 CFR 153.2 - Definitions and acronyms.

Code of Federal Regulations, 2014 CFR

2014-10-01

... viscosity NLS includes high viscosity Category B NLS and high viscosity Category C NLS. High viscosity Category B NLS means any Category B NLS having a viscosity of at least 25 mPa.s at 20 °C and at least 25 mPa.s at the time it is unloaded. High viscosity Category C NLS means any Category C NLS having a...

28 CFR 41.31 - Handicapped person.

Code of Federal Regulations, 2011 CFR

2011-07-01

... having, a mental or physical impairment that substantially limits one or more major life activities. (4...) Has a physical or mental impairment that substantially limits major life activities only as a result... Persons § 41.31 Handicapped person. (a) Handicapped person means any person who has a physical or mental...

Synergy of two low-affinity NLSs determines the high avidity of influenza A virus nucleoprotein NP for human importin α isoforms.

PubMed

Wu, Wei; Sankhala, Rajeshwer S; Florio, Tyler J; Zhou, Lixin; Nguyen, Nhan L T; Lokareddy, Ravi K; Cingolani, Gino; Panté, Nelly

2017-09-12

The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. Through site-directed mutagenesis and functional analysis, we found that NLS2 is also monopartite and is indispensable for viral infection. Atomic structures of importin α bound to two variants of NLS2 revealed NLS2 primarily binds the major-NLS binding site of importin α, unlike NLS1 that associates with the minor NLS-pocket. Though peptides corresponding to NLS1 and NLS2 bind weakly to importin α, the two NLSs synergize in the context of the full length NP to confer high avidity for importin α7, explaining why the virus efficiently replicates in the respiratory tract that exhibits high levels of this isoform. This study, the first to functionally characterize NLS2, demonstrates NLS2 plays an important and unexpected role in influenza A virus infection. We propose NLS1 and NLS2 form a bipartite NLS in trans, which ensures high avidity for importin α7 while preventing non-specific binding to viral RNA.

Ethics and human rights issues experienced by nurses in leadership roles.

PubMed

Redman, Barbara A; Fry, Sara T

2003-01-01

The aims of this study were to identify (1) the ethics and human rights issues experienced by nurses in leadership roles (NLs); (2) how frequently these issue occurred in the NLs'practices; and (3) how disturbed the NLs were by the issues. Dillman's Total Design Method (1978) for mailed surveys guided the study design. Data analysis was performed on 470 questionnaires from New England RNs in nursing leadership roles. The most frequently experienced ethics and human rights issues during the previous 12 months were (1) protecting patient right and human dignity; (2) respecting or not respecting informed consent to treatment; (3) use or nonuse of physical or chemical restraints; (4) providing care with possible risks to the RN's health; (5) following or not following advance directives; and (6) staffing patterns that limit patient access to nursing care. The most disturbing ethics and human rights issues experienced by the NLs were staffing patterns that limited patient access to nursing care, prolonging the dying process with inappropriate measures, working with unethical, incompetent, or impaired colleagues, implementing managed care policies that threaten quality of care, not considering quality of the patient's life, and caring for patients and families who are uninformed or misinformed about treatment, prognosis, or medical alternatives. Nearly 39% of the NLs reported experiencing ethics and human rights issues one to four times a week or more, and more than 90% handled their most recent ethics issue by discussing it with nursing peers. Study findings have implications for ethics education and resource support for nurses in leadership roles, and for further research on how NLs handle ethics and human rights issues in the workplace.

Library Services to the Blind and Physically Handicapped.

ERIC Educational Resources Information Center

De Cleene, Clare

This report describes library services for the blind and physically handicapped supported by the Library Services and Construction Act (LSCA), Title I, funds during fiscal 1985. Trends in library services for the blind and physically handicapped determined by an examination of reports from individual states are briefly summarized for the areas of…

Books for the Blind and Physically Handicapped. Fourth Edition.

ERIC Educational Resources Information Center

Haycraft, Howard

The article discusses library services available for the blind and the physically handicapped at the Division for the Blind and Physically Handicapped of the Library of Congress and at regional libraries. Topics covered include conditions and eligibility for service, with talking book service provided for individuals who cannot read or use…

Student Aides for Handicapped College Students. Final Report and Manual.

ERIC Educational Resources Information Center

Urie, Robert M.; And Others

The stated purpose of the project was to demonstrate that the use of student aides to assist selected physically handicapped students in the college setting, in conjunction with special physical facilities and individual counseling sessions for both the physically handicapped and the student aides, would result in a more satisfactory academic,…

A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1.

PubMed

Chen, Kang; Guo, Lingling; Zhang, Jiulong; Chen, Qing; Wang, Kuanglei; Li, Chenxi; Li, Weinan; Qiao, Mingxi; Zhao, Xiuli; Hu, Haiyang; Chen, Dawei

2017-01-15

In the present report, a degradable gene delivery system (PAMS/DNA/10NLS) containing nucleus location signal peptide (NLS) was prepared. The agarose gel electrophoresis, particle size and zeta potential of PAMS/DNA/10NLS were similar to those of PAMS/DNA, which proved that NLS did not affect the interaction between PAMS and DNA. PAMS/DNA/10NLS exhibited marked extracellular and intracellular degradation under acidic conditions. The degradation was believed to allow NLS to come into contact with importins easily, which was able to mediate the nucleus import. With the help of NLS, PAMS/DNA/10NLS exhibited a higher transfection capability than PAMS/DNA. Moreover, the transfection of PAMS/DNA/10NLS was less dependent on the breakdown of the nucleus envelope than PAMS/DNA. Considering that GTPase-activating protein 1 (RanGAP1) was able to activate the endogenous GTPase, which was necessary for NLS-mediated nucleus import, RanGAP1 overexpressed cells (RanGAP1 cells) were produced. This result showed that RanGAP1 cells had higher GTPase activities than normal cells. Both the nucleus import and transfection efficiency of PAMS/DNA/10NLS were markedly higher in RanGAP1 cells than that in normal cells. The in vivo transfection results also showed that the transfection efficiency of PAMS/DNA/10NLS was higher in RanGAP1 pre-treated mice than that in normal mice. These findings showed that PAMS/DNA/10NLS is a promising gene delivery system with the assistance of RanGAP1. The present report describes the increased transfection efficiency of a degradable gene delivery system (PAMS/DNA/10NLS) containing nuclear location signal (NLS) with the assistance of GTPase-activating protein 1 (RanGAP1). The physicochemical properties of PAMS/DNA/10NLS were similar to those of PAMS/DNA. PAMS/DNA/10NLS exhibited great extracellular and intracellular degradations, which might allow NLS to contact with importins easily. With the help of NLS, PAMS/DNA/10NLS exhibited a higher transfection capability than PAMS/DNA. The transfection of PAMS/DNA/10NLS had less dependence on the breakdown of nuclear envelope. Both the nuclear import and transfection efficiency of PAMS/DNA/10NLS were higher in RanGAP1 overexpressed cells than that in normal cells. Moreover, the transfection efficiency of PAMS/DNA/10NLS was higher in RanGAP1 pre-treated mice than that in normal mice. Copyright © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Determinants of handicap after stroke: the North East Melbourne Stroke Incidence Study (NEMESIS).

PubMed

Sturm, Jonathan W; Donnan, Geoffrey A; Dewey, Helen M; Macdonell, Richard A L; Gilligan, Amanda K; Thrift, Amanda G

2004-03-01

Handicap, although more relevant to the patient than impairment or disability, has received little attention in people with stroke. The aim of this study was to identify, in an unselected population, factors determining handicap at 2 years after stroke. All first-ever cases of stroke in a population of 306 631 over a 1-year period were assessed. Stroke severity, comorbidity, and demographic information was recorded. Among survivors, 2-year poststroke handicap was assessed with the London Handicap Scale. Disability, physical impairment, depression, anxiety, living arrangements, and recurrent stroke at 2 years were documented. If necessary, proxy assessments were obtained, except for mood. Linear regression analyses were performed to identify factors independently associated with handicap. First, all assessments (proxy and nonproxy) were examined; then, the nonproxy assessments were used to examine the effects of mood. Of 266 patients with incident stroke who were alive at 2 years, 226 (85%) were assessed. Significant determinants of handicap on univariable analysis were age, female sex, socioeconomic status, alcohol intake, stroke subtype, initial stroke severity; 2-year physical impairment, disability, depression and anxiety scores; institutionalization; and recurrent stroke. On multivariable analysis, the independent determinants of handicap were age and 2-year physical impairment and disability. In analysis restricted to nonproxy data, depression and anxiety were also independently associated with handicap. Age, concurrent disability, and physical impairment were more important determinants of handicap than other demographic factors or initial stroke severity. Because depression and anxiety were independently associated with handicap, their treatment may potentially reduce handicap in stroke patients.

Transformation of sweet orange [Citrus sinensis (L.) Osbeck] with pthA-nls for acquiring resistance to citrus canker disease.

PubMed

Yang, Li; Hu, Chunhua; Li, Na; Zhang, Jiayin; Yan, Jiawen; Deng, Ziniu

2011-01-01

The COOH terminal of pthA encoding three nuclear localizing signals (NLS) was amplified by polymerase chain reaction (PCR) from the plasmid of Xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. Then the sense and antisense strands of the nls were cloned into pBI121 vector. pthA-nls driven by the CaMV35 s promoter was transferred into sweet orange via Agrobacterium -mediated transformation. Successful integration was confirmed by PCR and Southern blotting, and 12 sense-nls (nls (+)) and 9 antisense-nls (nls (-)) transgenic clones were obtained. The expression of nls fragment was analyzed by RT-PCR, Real time q-PCR and Western blotting, in which the specific NLS protein was detected only in nls (+) transgenic clones. In an in vitro assay, when pin-puncture inoculation was performed with 2.5 × 10(7) cfu/ml of bacterial solution, the nls (+) transgenic clones showed no typical lesion development, while typical symptoms were observed in the wild types and the nls (-) transgenic clones. In vivo assay results indicated that the nls (+) transgenic clones showed less disease incidence, in comparison with the wild types and the nls (-) transgenic clones, when pin-puncture inoculation was performed with 10(4)-10(5) cfu/ml. The minimum disease incidence was 23.3% for 'Sucarri' sweet orange and 33.3% for 'Bingtang' sweet orange. When 10(4)-10(7) cfu/ml of pathogen was spray inoculated, the nls (+) transgenic clones did not show any symptom, and even the concentration raised to 10(9) cfu/ml, the disease incidence was 20-80%, while the wild types and the nls (-) transgenic clones had 100% disease development with whatever concentration of inoculum. Two transgenic clones were confirmed to be resistant to citrus canker disease in the repeated inoculation. The results suggested that the transformation of nls sense strands may offer an effective way to acquire resistance to citrus canker disease.

NLS-RARα promotes proliferation and inhibits differentiation in HL-60 cells.

PubMed

Hu, Xiu-Xiu; Zhong, Liang; Zhang, Xi; Gao, Yuan-Mei; Liu, Bei-Zhong

2014-01-01

A unique mRNA produced in leukemic cells from a t(15;17) acute promyelocytic leukemia (APL) patient encodes a fusion protein between the retinoic acid receptor α (RARα) and a myeloid gene product called PML. Studies have reported that neutrophil elastase (NE) cleaves bcr-1-derived PML-RARα in early myeloid cells, leaving only the nuclear localization signal (NLS) of PML attached to RARα. The resultant NLS-RARα fusion protein mainly localizes to, and functions within, the cell nucleus. It is speculated that NLS-RARα may act in different ways from the wild-type RARα, but its biological characteristics have not been reported. This study takes two approaches. Firstly, the NLS-RARα was silenced with pNLS-RARα-shRNA. The mRNA and protein expression of NLS-RARα were detected by RT-PCR and Western blot respectively. Cell proliferation in vitro was assessed by MTT assay. Flow cytometry (FCM) was used to detect the differentiation of cells. Secondly, the NLS-RARα was over-expressed by preparation of recombinant adenovirus HL-60/pAd-NLS-RARα. The assays of mRNA and protein expression of NLS-RARα, and cell proliferation, were as above. By contrast, cell differentiation was stimulated by all trans retinoic acid (ATRA) (2.5µmol/L) at 24h after virus infection of pAd-NLS-RARα, and then detected by CD11b labeling two days later. The transcription and translation of C-MYC was detected in HL-60/pAd-NLS-RARα cells which treated by ATRA. Our results showed that compared to the control groups, the expression of NLS-RARα was significantly reduced in the HL-60/pNLS-RARα-shRNA cells, and increased dramatically in the HL-60/pAd-NLS-RARα cells. The proliferation was remarkably inhibited in the HL-60/pNLS-RARα-shRNA cells in a time-dependent manner, but markedly promoted in the HL-60/pAd-NLS-RARα cells. FCM outcome revealed the differentiation increased in HL-60/pNLS-RARα-shRNA cells, and decreased in the HL-60/pAd-NLS-RARα cells treated with 2.5µmol/L ATRA. The expression of C-MYC increased strikingly in HL-60/pAd-NLS-RARα cells treated with 2.5µmol/L ATRA. Down-regulation of NLS-RARα expression inhibited the proliferation and induced the differentiation of HL-60 cells. On the contrary, over-expression of NLS-RARα promoted proliferation and reduced the ATRA-induced differentiation of HL-60 cells.

A spectroscopic analysis of a sample of narrow-line Seyfert 1 galaxies selected from the Sloan Digital Sky Survey

NASA Astrophysics Data System (ADS)

Cracco, V.; Ciroi, S.; Berton, M.; Di Mille, F.; Foschini, L.; La Mura, G.; Rafanelli, P.

2016-10-01

We revisited the spectroscopic characteristics of narrow-line Seyfert 1 galaxies (NLS1s) by analysing a homogeneous sample of 296 NLS1s at redshift between 0.028 and 0.345, extracted from the Sloan Digital Sky Survey (SDSS-DR7) public archive. We confirm that NLS1s are mostly characterized by Balmer lines with Lorentzian profiles, lower black hole masses and higher Eddington ratios than classic broad-line Seyfert 1 (BLS1s), but they also appear to be active galactic nuclei (AGNs) contiguous with BLS1s and sharing with them common properties. Strong Fe II emission does not seem to be a distinctive property of NLS1s, as low values of Fe II/Hβ are equally observed in these AGNs. Our data indicate that Fe II and Ca II kinematics are consistent with the one of Hβ. On the contrary, O I λ8446 seems to be systematically narrower and it is likely emitted by gas of the broad-line region more distant from the ionizing source and showing different physical properties. Finally, almost all NLS1s of our sample show radial motions of the narrow-line region highly ionized gas. The mechanism responsible for this effect is not yet clear, but there are hints that very fast outflows require high continuum luminosities (>1044 erg s-1) or high Eddington ratios (log (Lbol/LEdd) > -0.1).

Evolutionary gradient of predicted nuclear localization signals (NLS)-bearing proteins in genomes of family Planctomycetaceae.

PubMed

Guo, Min; Yang, Ruifu; Huang, Chen; Liao, Qiwen; Fan, Guangyi; Sun, Chenghang; Lee, Simon Ming-Yuen

2017-04-04

The nuclear envelope is considered a key classification marker that distinguishes prokaryotes from eukaryotes. However, this marker does not apply to the family Planctomycetaceae, which has intracellular spaces divided by lipidic intracytoplasmic membranes (ICMs). Nuclear localization signal (NLS), a short stretch of amino acid sequence, destines to transport proteins from cytoplasm into nucleus, and is also associated with the development of nuclear envelope. We attempted to investigate the NLS motifs in Planctomycetaceae genomes to demonstrate the potential molecular transition in the development of intracellular membrane system. In this study, we identified NLS-like motifs that have the same amino acid compositions as experimentally identified NLSs in genomes of 11 representative species of family Planctomycetaceae. A total of 15 NLS types and 170 NLS-bearing proteins were detected in the 11 strains. To determine the molecular transformation, we compared NLS-bearing protein abundances in the 11 representative Planctomycetaceae genomes with them in genomes of 16 taxonomically varied microorganisms: nine bacteria, two archaea and five fungi. In the 27 strains, 29 NLS types and 1101 NLS-bearing proteins were identified, principal component analysis showed a significant transitional gradient from bacteria to Planctomycetaceae to fungi on their NLS-bearing protein abundance profiles. Then, we clustered the 993 non-redundant NLS-bearing proteins into 181 families and annotated their involved metabolic pathways. Afterwards, we aligned the ten types of NLS motifs from the 13 families containing NLS-bearing proteins among bacteria, Planctomycetaceae or fungi, considering their diversity, length and origin. A transition towards increased complexity from non-planctomycete bacteria to Planctomycetaceae to archaea and fungi was detected based on the complexity of the 10 types of NLS-like motifs in the 13 NLS-bearing proteins families. The results of this study reveal that Planctomycetaceae separates slightly from the members of non-planctomycete bacteria but still has substantial differences from fungi, based on the NLS-like motifs and NLS-bearing protein analysis.

[Analysis of nuclear localization and signal function of MITF protein predisposing to Warrdenburg syndrome].

PubMed

Zhang, Hua; Feng, Juan; Chen, Hongsheng; Li, Jiada; Luo, Hunjin; Feng, Yong

2015-12-01

To study the role of dysfunction of nuclear localization signals (NLS) of MITF protein in the pathogenesis of Waardenburg syndrome. Eukaryotic expression plasmid pCMV-MITF-Flag was used as a template to generate mutant plasmid pCMV-MITF△NLS-Flag by molecular cloning technique in order to design the mutagenic primers. The UACC903 cells were transfected transiently with MITF and MITF△NLS plasmids, and the luciferase activity assays were performed to determine their impact on the transcriptional activities of target gene tyrosinase (TYR). The oligonucleotide 5'-GAACGAAGAAGAAGATTT-3' was subcloned into pEGFP-N1 to generate recombinant eukaryotic expression plasmid pEGFP-N1-MITF-NLS. The NIH3T3 cells were transfected separately with MITF, MITF△NLS, pEGFP-N1 and pEGFP-N1-NLS plasmids, and their subcellular distribution was observed by immunoflorescence assays. Expression plasmids for the mutant MITF△NLS with loss of core NLS sequence and pEGFP-N1-NLS coupled with MITF△NLS were successfully generated. Compared with the wild-type MITF, MITF△NLS was not able to transactivate the transcriptional activities of promoter TYR and did not affect the normal function of MITF. MITF△NLS was only localized in the cytoplasm and pEGFP-N1 was found in both the cytoplasm and nucleus, whereas pEGFP-N1-NLS was mainly located in the nucleus. This study has confirmed the localization function of NLS sequence 213ERRRRF218 within the MITF protein. Mutant MITF with loss of NLS has failed to transactivate the transcriptional activities of target gene TYR, which can result in melanocyte defects and cause WS.

24 CFR 8.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-04-01

... NONDISCRIMINATION BASED ON HANDICAP IN FEDERALLY ASSISTED PROGRAMS AND ACTIVITIES OF THE DEPARTMENT OF HOUSING AND... altered, can be approached, entered, and used by individuals with physical handicaps. The phrase... individuals with physical handicaps. A unit that is on an accessible route and is adaptable and otherwise in...

Minimizing Teacher Stress. Structuring Positive Interactions for Handicapped and Nonhandicapped Children in Physical Education.

ERIC Educational Resources Information Center

Colvin, Nola R.; And Others

1982-01-01

Components of an integrated physical education program, which consists of handicapped and nonhandicapped students, include: (1) activities that promote interaction among all students; (2) strategies that place handicapped and nonhandicapped students together; and (3) reinforcement of cooperative behavior. (CJ)

Four nucleocytoplasmic-shuttling proteins and p53 interact specifically with the YB-NLS and are involved in anticancer reagent-induced nuclear localization of YB-1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tanaka, Toru; Ohashi, Sachiyo; Kobayashi, Shunsuke

In cancer cells, anticancer reagents often trigger nuclear accumulation of YB-1, which participates in the progression of cancer malignancy. YB-1 has a non-canonical nuclear localization signal (YB-NLS). Here we found that four nucleocytoplasmic-shuttling RNA-binding proteins and p53 interact specifically with the YB-NLS and co-accumulate with YB-1 in the nucleus of actinomycin D-treated cells. To elucidate the roles of these YB-NLS-binding proteins, we performed a dominant-negative experiment in which a large excess of YB-NLS interacts with the YB-NLS-binding proteins, and showed inhibitory effects on actinomycin D-induced nuclear transport of endogenous YB-1 and subsequent MDR1 gene expression. Furthermore, the YB-NLS-expressing cells weremore » also found to show increased drug sensitivity. Our results suggest that these YB-NLS-associating proteins are key factors for nuclear translocation/accumulation of YB-1 in cancer cells. - Highlights: • Four nucleocytoplasmic-shuttling proteins and p53 associate with YB-NLS. • They showed nuclear co-accumulation with YB-1 in actinomycin D-treated cells. • Overexpression of YB-NLS was carried out to take YB-NLS-binding proteins from YB-1. • YB-NLS inhibited actinomycin D-induced nuclear localization of endogenous YB-1. • YB-NLS suppressed actinomycin D-induced expression of MDR1.« less

Visible and Near-Infrared Spectroscopy of Seyfert 1 and Narrow-Line Seyfert 1 Galaxies

NASA Astrophysics Data System (ADS)

Rodríguez-Ardila, Alberto; Pastoriza, Miriani G.; Donzelli, Carlos J.

2000-01-01

This paper studies the continuum and emission-line properties of a sample composed of 16 normal Seyfert 1 and seven narrow-line Seyfert 1 (NLS1) galaxies using optical and near-IR CCD spectroscopy. The continuum emission of the galaxies can be described in terms of a combination of stellar population, a nonstellar continuum of power-law form, and Fe II emission. A significative difference in the optical spectral index between NLS1's and normal Seyfert 1's is observed; the latter is steeper. Most NLS1's show Fe II/Hβ ratios larger than those observed in the other Seyfert 1's. In the IRAS band, both groups of galaxies have very similar properties. We have searched for the presence of optically thin gas in the broad-line region (BLR) of the galaxies by comparing the broad O I λ8446 and Hα emission-line profiles. Our analysis show that in the NLS1's, both profiles are similar in shape and width. This result contradicts the hypothesis of thin gas emission in the high-velocity part of the BLR to explain the ``narrowness'' of broad optical permitted lines in these objects. Evidence of narrow O I λ8446 emission is found in six galaxies of our sample, implying that this line is not restricted to a pure BLR phenomenon. In the narrow-line region, we find similar luminosities in the permitted and high-ionization lines of NLS1's and normal Seyfert 1's. However, low-ionization lines such as [O I] λ6300, [O II] λ3727, and [S II] λλ6717, 6731 are intrinsically less luminous in NLS1's. Physical properties derived from density- and temperature-sensitive line ratios suggest that the [O II] and [S II] emitting zones are overlapping in normal Seyfert 1's and separated in NLS1's. Based on observations made at CASLEO. Complejo Astronómico El Leoncito (CASLEO) is operated under agreement between the Consejo Nacional de Investigaciones Científicas y técnicas de la República Argentina and the National Universities of La Plata, Córdoba and San Juán.

Teaching the Physically Handicapped to Swim.

ERIC Educational Resources Information Center

Anderson, William

First principles of teaching swimming to the handicapped are reviewed; attention is given to children with cerebral palsy or muscular dystrophy, physical handicaps, blindness, and deafness. Swimming strokes, suggested exercises, group teaching, and a typical sequence of lessons and exercises are considered. Some case histories and a plan for a…

Yeast Nucleoporins Involved in Passive Nuclear Envelope Permeability

PubMed Central

Shulga, Nataliya; Mosammaparast, Nima; Wozniak, Richard; Goldfarb, David S.

2000-01-01

The vertebrate nuclear pore complex (NPC) harbors an ∼10-nm diameter diffusion channel that is large enough to admit 50-kD polypeptides. We have analyzed the permeability properties of the Saccharomyces cerevisiae nuclear envelope (NE) using import (NLS) and export (NES) signal-containing green fluorescent protein (GFP) reporters. Compared with wild-type, passive export rates of a classical karyopherin/importin (Kap) Kap60p/Kap95p-targeted NLS-GFP reporter (cNLS-GFP) were significantly faster in nup188-Δ and nup170-Δ cells. Similar results were obtained using two other NLS-GFP reporters, containing either the Kap104p-targeted Nab2p NLS (rgNLS) or the Kap121p-targeted Pho4p NLS (pNLS). Elevated levels of Hsp70 stimulated cNLS-GFP import, but had no effect on the import of rgNLS-GFP. Thus, the role of Hsp70 in NLS-directed import may be NLS- or targeting pathway-specific. Equilibrium sieving limits for the diffusion channel were assessed in vivo using NES-GFP reporters of 36–126 kD and were found to be greater than wild-type in nup188-Δ and nup170-Δ cells. We propose that Nup170p and Nup188p are involved in establishing the functional resting diameter of the NPC's central transport channel. PMID:10831607

Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro: Implications for the role of NLS in viral replication

PubMed Central

2011-01-01

Background Porcine circovirus type 2 (PCV2) is believed to be the primary causative agent of postweaning multisystemic wasting syndrome (PMWS). It is supposed that capsid protein of PCV may contribute to replication control via interaction between Cap and Rep in the nucleoplasm. In this study, we described the construction and in vitro characterization of NLS-exchanged PCV DNA clones based on a PMWS-associated PCV2b isolate from China to determine the role of ORF2 NLS in PCV replication. Results The PCV1, PCV2, PCV2-NLS1 and PCV1-NLS2 DNA clone were generated by ligating a copy of respective genome in tandem with a partial duplication. The PCV2-NLS1 and PCV1-NLS2 DNA clone contained a chimeric genome in which the ORF2 NLS was exchanged. The four DNA clones were all confirmed to be infectious in vitro when transfected into PK-15 cells, as PCV capsid protein were expressed in approximately 10-20% of the transfected cells. The in vitro growth characteristics of the DNA clones were then determined and compared. All the recovered progeny viruses gave rise to increasing infectious titers during passages and were genetically stable by genomic sequencing. The chimeric PCV1-NLS2 and PCV2-NLS1 viruses had the final titers of about 104.2 and 103.8 TCID50/ml, which were significantly lower than that of PCV1 and PCV2 (105.6 and 105.0 TCID50/ml, respectively). When the ORF2 NLS exchanged, the mutant PCV2 (PCV2-NLS1) still replicated less efficiently and showed lower infectious titer than did PCV1 mutant (PCV1-NLS2), which was consistent with the distinction between wild type PCV1 and PCV2. Conclusions Recovery of the chimeiric PCV1-NLS2 and PCV2-NLS1 progeny viruses indicate that the nuclear localization signal sequence of capsid protein are functionally exchangeable between PCV1 and PCV2 with respect to the role of nuclear importing and propagation. The findings also reveal that ORF2 NLS play an accessory role in the replication of PCV. However, we found that ORF2 NLS was not responsible for the distinction of in vitro growth characteristic between PCV1 and PCV2. Further studies are required to determine the in vivo viral replication and pathogenicity of the NLS chimeric DNA clones. PMID:21733152

Implicit theory of athletic ability and self-handicapping in college students.

PubMed

Chen, Lung Hung; Chen, Mei-Yen; Lin, Meng-Shyan; Kee, Ying Hwa; Kuo, Chin Fang; Shui, Shang-Hsueh

2008-10-01

Self-handicapping is a maladaptive behavior which undermines students' achievements, but the antecedents of self-handicapping are not well studied in physical education. The aim was to examine the relations of implicit theory of abilities and self-handicapping in physical education. 264 college students, whose mean age was 20.3 yr. (SD = 1.7), completed the Conceptions of the Nature of Athletic Ability Questionnaire-2 and Self-handicapping Scale for Sport. Analysis indicated entity beliefs positively predicted reduced effort and making excuses. Also, incremental beliefs negatively predicted reduced effort. Results are discussed in terms of implicit theory of ability and self-handicapping. Directions for research and implications are stated.

Considerations for Integration of Physically Handicapped and Non-Handicapped Preschool Children.

ERIC Educational Resources Information Center

Cormack, Elizabeth O.

Eight physically handicapped (blind, spina bifida, cerebral palsy, or genetic defect) preschool children and eight children with normal sensory motor abilities (mean age of both groups 3 1/2 years) participated in a preschool demonstration project designed to promote equal educational and social experiences in an integrated preschool through the…

Distance Teaching and the Visually Handicapped: Some New Developments Using Synthetic Speech.

ERIC Educational Resources Information Center

Vincent, A. T.

1983-01-01

Distance education is important for many physically handicapped students where attendance at a conventional institution is not possible, either because of their physical condition or for reasons in common with the nonhandicapped. This paper examines an area of disability, visual handicap, and examines research into the use of microelectronic…

Competency-Based Training Program--Teachers of Physical Education for Handicapped Individuals. Summer Institute Final Report, 1986-1990.

ERIC Educational Resources Information Center

Cheatum, Billye Ann

This federally funded project's goals were to provide a competency-based training program that would enable Western Michigan University graduate and undergraduate students to meet Michigan's endorsement requirements for teaching physical education to handicapped individuals, and to provide direct services to handicapped children in the Kalamazoo…

45 CFR 84.47 - Nonacademic services.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and... applies may not discriminate on the basis of handicap. A recipient that offers physical education courses... may offer to handicapped students physical education and athletic activities that are separate or...

22 CFR 142.47 - Non-academic services.

Code of Federal Regulations, 2010 CFR

2010-04-01

... services. (a) Physical education and athletics. (1) In providing physical education courses, athletics and... not discriminate on the basis of handicap. A recipient that offers physical education courses or that... handicapped students physical education and athletic activities that are separate or different only if...

22 CFR 217.47 - Nonacademic services.

Code of Federal Regulations, 2010 CFR

2010-04-01

... services. (a) Physical education and athletics. (1) In providing physical education courses and athletics... applies may not discriminate on the basis of handicap. A recipient that offers physical education courses... may offer to handicapped students physical education and athletic activities that are separate or...

46 CFR 153.2 - Definitions and acronyms.

Code of Federal Regulations, 2012 CFR

2012-10-01

... materials that are transported as bulk liquids by water in § 153.40. High viscosity NLS includes high viscosity Category B NLS and high viscosity Category C NLS. High viscosity Category B NLS means any Category B NLS having a viscosity of at least 25 mPa.s at 20 °C and at least 25 mPa.s at the time it is...

46 CFR 153.2 - Definitions and acronyms.

Code of Federal Regulations, 2011 CFR

2011-10-01

... materials that are transported as bulk liquids by water in § 153.40. High viscosity NLS includes high viscosity Category B NLS and high viscosity Category C NLS. High viscosity Category B NLS means any Category B NLS having a viscosity of at least 25 mPa.s at 20 °C and at least 25 mPa.s at the time it is...

Searches for H2O masers toward narrow-line Seyfert 1 galaxies

NASA Astrophysics Data System (ADS)

Yoshiaki, Hagiwara; Doi, Akihiro; Hachisuka, Kazuya; Horiuchi, Shinji

2018-05-01

We present searches for 22 GHz H2O masers toward 36 narrow-line Seyfert 1 galaxies (NLS1s), selected from known NLS1s with vsys ≲ 41000 km s-1. Out of the 36 NLS1s in our sample, 11 have been first surveyed in our observations, while the observations of other NLS1s were previously reported in literature. In our survey, no new water maser source from NLS1s was detected at the 3σ rms level of 8.4 mJy to 144 mJy, which depends on different observing conditions or inhomogeneous sensitivities of each observation using three different telescopes. It is likely that the non-detection of new masers in our NLS1 sample is primarily due to insufficient sensitivities of our observations. Including the five known NLS1 masers, the total detection rate of the H2O maser in NLS1s is not remarkably different from that of type 2 Seyfert galaxies or LINERs. However, more extensive and systematic searches of NLS1 would be required for a statistical discussion of the detection rate of the NLS1 maser, compared with that of type 2 Seyferts or LINERs.

Searches for H2O masers toward narrow-line Seyfert 1 galaxies

NASA Astrophysics Data System (ADS)

Hagiwara, Yoshiaki; Doi, Akihiro; Hachisuka, Kazuya; Horiuchi, Shinji

2018-06-01

We present searches for 22 GHz H2O masers toward 36 narrow-line Seyfert 1 galaxies (NLS1s), selected from known NLS1s with vsys ≲ 41000 km s-1. Out of the 36 NLS1s in our sample, 11 have been first surveyed in our observations, while the observations of other NLS1s were previously reported in literature. In our survey, no new water maser source from NLS1s was detected at the 3σ rms level of 8.4 mJy to 144 mJy, which depends on different observing conditions or inhomogeneous sensitivities of each observation using three different telescopes. It is likely that the non-detection of new masers in our NLS1 sample is primarily due to insufficient sensitivities of our observations. Including the five known NLS1 masers, the total detection rate of the H2O maser in NLS1s is not remarkably different from that of type 2 Seyfert galaxies or LINERs. However, more extensive and systematic searches of NLS1 would be required for a statistical discussion of the detection rate of the NLS1 maser, compared with that of type 2 Seyferts or LINERs.

Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kitamura, Akira; Nakayama, Yusaku; Kinjo, Masataka, E-mail: kinjo@sci.hokudai.ac.jp

2015-07-31

Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells. However, the detailed mechanism of nuclear retention of GFP-NLS remains unclear. Here, we show that a candidate mechanism for the strong nuclear retention of GFP-NLS is via the RNA-binding ability of the NLS sequence. GFP tagged with a classical NLS derived from Simian virus 40 (GFP-NLS{sup SV40}) localized not only in the nucleoplasm, but also to the nucleolus, the nuclear subdomain in which ribosome biogenesismore » takes place. GFP-NLS{sup SV40} in the nucleolus was mobile, and intriguingly, the diffusion coefficient, which indicates the speed of diffusing molecules, was 1.5-fold slower than in the nucleoplasm. Fluorescence correlation spectroscopy (FCS) analysis showed that GFP-NLS{sup SV40} formed oligomers via RNA binding, the estimated molecular weight of which was larger than the limit for passive nuclear export into the cytoplasm. These findings suggest that the nuclear localization of GFP-NLS{sup SV40} likely results from oligomerization mediated via RNA binding. The analytical technique used here can be applied for elucidating the details of other nuclear localization mechanisms, including those of several types of nuclear proteins. In addition, GFP-NLS{sup SV40} can be used as an excellent marker for studying both the nucleoplasm and nucleolus in living cells. - Highlights: • Nuclear localization signal-tagged GFP (GFP-NLS) showed clear nuclear localization. • The GFP-NLS dynamically localized not only in the nucleoplasm, but also to the nucleolus. • The nuclear localization of GFP-NLS results from transient oligomerization mediated via RNA binding. • Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus.« less

Expanding the Definition of the Classical Bipartite Nuclear Localization Signal

PubMed Central

Lange, Allison; McLane, Laura M.; Mills, Ryan E.; Devine, Scott E.; Corbett, Anita H.

2010-01-01

Nuclear localization signals (NLSs) are amino acid sequences that target cargo proteins into the nucleus. Rigorous characterization of NLS motifs is essential to understanding and predicting pathways for nuclear import. The best-characterized NLS is the classical NLS (cNLS), which is recognized by the cNLS receptor, importin-α. cNLSs are conventionally defined as having one (monopartite) or two clusters of basic amino acids separated by a 9-12 amino acid linker (bipartite). Motivated by the finding that Ty1 integrase, which contains an unconventional putative bipartite cNLS with a 29 amino acid linker, exploits the classical nuclear import machinery, we assessed the functional boundaries for linker length within a bipartite cNLS. We confirmed that the integrase cNLS is a bona fide bipartite cNLS, then carried out a systematic analysis of linker length in an obligate bipartite cNLS cargo, which revealed that some linkers longer than conventionally defined can function in nuclear import. Linker function is dependent on the sequence and likely the inherent flexibility of the linker. Subsequently, we interrogated the Saccharomyces cerevisiae proteome to identify cellular proteins containing putative long bipartite cNLSs. We experimentally confirmed that Rrp4 contains a bipartite cNLS with a 25 amino acid linker. Our studies reveal that the traditional definition of bipartite cNLSs is too restrictive and linker length can vary depending on amino acid composition PMID:20028483

Radio-emitting narrow-line Seyfert 1 galaxies in the JVLA perspective

NASA Astrophysics Data System (ADS)

Berton, M.; Congiu, E.; Järvelä, E.; Antonucci, R.; Kharb, P.; Lister, M. L.; Tarchi, A.; Caccianiga, A.; Chen, S.; Foschini, L.; Lähteenmäki, A.; Richards, J. L.; Ciroi, S.; Cracco, V.; Frezzato, M.; La Mura, G.; Rafanelli, P.

2018-06-01

We report the first results of a survey on 74 narrow-line Seyfert 1 galaxies (NLS1s) carried out in 2015 with the Karl G. Jansky Very Large Array (JVLA) at 5 GHz in A-configuration. So far, this is the largest survey aimed to image the radio continuum of NLS1s. We produced radio maps in order to compare the general properties of three different samples of objects: radio-quiet NLS1s (RQNLS1s), steep-spectrum radio-loud NLS1s (S-NLS1s), and flat-spectrum radio-loud NLS1s (F-NLS1s). We find that the three classes correspond to different radio morphologies, with F-NLS1s being more compact, and RQNLS1s often showing diffuse emission on kpc scales. We also find that F-NLS1s might be low-luminosity and possibly young blazars, and that S-NLS1s are part of the parent population of F-NLS1s. Dedicated studies to RQNLS1s are needed to fully understand their role in the unification pictures. The reduced images (FITS files) are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/614/A87

Nonlinear Schroedinger Approximations for Partial Differential Equations with Quadratic and Quasilinear Terms

NASA Astrophysics Data System (ADS)

Cummings, Patrick

We consider the approximation of solutions of two complicated, physical systems via the nonlinear Schrodinger equation (NLS). In particular, we discuss the evolution of wave packets and long waves in two physical models. Due to the complicated nature of the equations governing many physical systems and the in-depth knowledge we have for solutions of the nonlinear Schrodinger equation, it is advantageous to use approximation results of this kind to model these physical systems. The approximations are simple enough that we can use them to understand the qualitative and quantitative behavior of the solutions, and by justifying them we can show that the behavior of the approximation captures the behavior of solutions to the original equation, at least for long, but finite time. We first consider a model of the water wave equations which can be approximated by wave packets using the NLS equation. We discuss a new proof that both simplifies and strengthens previous justification results of Schneider and Wayne. Rather than using analytic norms, as was done by Schneider and Wayne, we construct a modified energy functional so that the approximation holds for the full interval of existence of the approximate NLS solution as opposed to a subinterval (as is seen in the analytic case). Furthermore, the proof avoids problems associated with inverting the normal form transform by working with a modified energy functional motivated by Craig and Hunter et al. We then consider the Klein-Gordon-Zakharov system and prove a long wave approximation result. In this case there is a non-trivial resonance that cannot be eliminated via a normal form transform. By combining the normal form transform for small Fourier modes and using analytic norms elsewhere, we can get a justification result on the order 1 over epsilon squared time scale.

Correlates and importance of neglect-like symptoms in complex regional pain syndrome.

PubMed

Wittayer, Matthias; Dimova, Violeta; Birklein, Frank; Schlereth, Tanja

2018-05-01

Neglect-like symptoms (NLS) are frequently observed in complex regional pain syndrome (CRPS). The clinical meaning of NLS, however, is largely unknown. Therefore, this study sets out to assess the importance of NLS for patient outcome and to explore their clinical correlates. We assessed NLS in a group of 53 patients with CRPS and compared the results to 28 healthy volunteers. To define the origin of the NLS reports, we tested the subjective visual midline, performed a limb-laterality recognition test, and quantitative sensory testing. In addition, psychological and pain assessment scales were completed. Tests were analyzed with univariate and multivariate approaches. After 6 months, patients were reassessed and the influence of NLS on pain outcome was determined. Most patients reported NLS in the questionnaire, whereas subjective visual midline and limb-laterality recognition test in contrast to previous studies did not reveal perceptual disturbances. Neglect-like symptom scores were associated with pain and pain catastrophizing in acute CRPS and anxiety and thermal sensory loss in chronic CRPS. Furthermore, high NLS scores had a negative impact on pain outcome after 6 months. Our results indicate that NLS have a different meaning in acute and chronic CRPS and might be of prognostic value. Possibly, treatment should focus on reducing NLS.

A Comprehensive Physical Education Program for the Severely Physically Handicapped.

ERIC Educational Resources Information Center

DeBell, James

Described is an adaptive comprehensive physical education (PE) program (funded by Title III in 1968) for 48 severly physically handicapped senior high school students in Rochester, New York. Program aims are given to be providing a diversified program of developmental activities in a friendly environment to increase the physical, social, and…

Socially Mainstreaming Handicapped Students.

ERIC Educational Resources Information Center

Miller, Maurice; Loukellis, Irene

The basis for socially mainstreaming handicapped children as well as changing attitudes toward the handicapped are discussed; and a list of teaching activities and resources for introducing students to the mentally retarded, the visually impaired, the physically handicapped, the hearing impaired, and the learning disabled is presented. A summary…

Train in vain: the role of the self in claimed self-handicapping strategies.

PubMed

Finez, Lucie; Sherman, David K

2012-10-01

Two field studies investigate the role of self in the tendency of athletes to engage in claimed handicapping strategies during training (anticipatively claiming that handicaps may interfere with their performance). Study 1 tested the relationship between trait self-esteem and athletes' engagement in claimed self-handicapping. As hypothesized, low physical self-esteem athletes claimed more handicaps than high physical self-esteem athletes. For stronger evidence for the causal role of the self, Study 2 tested whether securing athletes' self-worth through self-affirmation would lead to decreased claimed self-handicapping by using a mixed model design that allows for both between-subjects (affirmation vs. control condition) and within-subject comparisons (before vs. after self-affirmation intervention). Self-affirmed athletes had decreased levels of claimed self-handicapping. Studies 1 and 2 also demonstrate that athletes engage in claimed self-handicapping during training, which could have deleterious effects on subsequent performance. Discussion centers on theoretical implications and applications for coaches, sport teachers, and sport psychologists.

The role of the National Launch System in support of Space Station Freedom

NASA Technical Reports Server (NTRS)

Green, J. L.; Saucillo, R. J.; Cirillo, W. M.

1992-01-01

A study was performed to determine the most appropriate potential use of the National Launch System (NLS) for Space Station Freedom (SSF) logistics resupply and growth assembly needs. Objectives were to estimate earth-to-SSF cargo requirements, identify NLS sizing trades, and assess operational constraints of a shuttle and NLS transportation infrastructure. Detailed NLS and Shuttle flight manifests were developed to model varying levels of NLS support. NLS delivery of SSF propellant, and in some cases, cryoenic fluids, yield significant shuttle flight savings with minimum impact to the baseline SSF design. Additional cargo can be delivered by the NLS if SSF trash disposal techniques are employed to limit return cargo requirements. A common vehicle performance level can be used for both logistics resupply and growth hardware delivery.

Computational Fluid Dynamic (CFD) analysis of axisymmetric plume and base flow of film/dump cooled rocket nozzle

NASA Technical Reports Server (NTRS)

Tucker, P. K.; Warsi, S. A.

1993-01-01

Film/dump cooling a rocket nozzle with fuel rich gas, as in the National Launch System (NLS) Space Transportation Main Engine (STME), adds potential complexities for integrating the engine with the vehicle. The chief concern is that once the film coolant is exhausted from the nozzle, conditions may exist during flight for the fuel-rich film gases to be recirculated to the vehicle base region. The result could be significantly higher base temperatures than would be expected from a regeneratively cooled nozzle. CFD analyses were conduced to augment classical scaling techniques for vehicle base environments. The FDNS code with finite rate chemistry was used to simulate a single, axisymmetric STME plume and the NLS base area. Parallel calculations were made of the Saturn V S-1 C/F1 plume base area flows. The objective was to characterize the plume/freestream shear layer for both vehicles as inputs for scaling the S-C/F1 flight data to NLS/STME conditions. The code was validated on high speed flows with relevant physics. This paper contains the calculations for the NLS/STME plume for the baseline nozzle and a modified nozzle. The modified nozzle was intended to reduce the fuel available for recirculation to the vehicle base region. Plumes for both nozzles were calculated at 10kFT and 50kFT.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kiriyama, Takao; Hirano, Makito; Asai, Hirohide

Triple A syndrome is an autosomal recessive neurological disease, mimicking motor neuron disease, and is caused by mutant ALADIN, a nuclear-pore complex component. We recently discovered that the pathogenesis involved impaired nuclear import of DNA repair proteins, including DNA ligase I and the cerebellar ataxia causative protein aprataxin. Such impairment was overcome by fusing classical nuclear localization signal (NLS) and 137-aa downstream sequence of XRCC1, designated stretched NLS (stNLS). We report here that the minimum essential sequence of stNLS (mstNLS) is residues 239-276, downsized by more than 100 aa. mstNLS enabled efficient nuclear import of DNA repair proteins in patientmore » fibroblasts, functioned under oxidative stress, and reduced oxidative-stress-induced cell death, more effectively than stNLS. The stress-tolerability of mstNLS was also exerted in control fibroblasts and neuroblastoma cells. These findings may help develop treatments for currently intractable triple A syndrome and other oxidative-stress-related neurological diseases, and contribute to nuclear compartmentalization study.« less

Chitosan Nanoparticles for Nuclear Targeting: The Effect of Nanoparticle Size and Nuclear Localization Sequence Density.

PubMed

Tammam, Salma N; Azzazy, Hassan M E; Breitinger, Hans G; Lamprecht, Alf

2015-12-07

Many recently discovered therapeutic proteins exert their main function in the nucleus, thus requiring both efficient uptake and correct intracellular targeting. Chitosan nanoparticles (NPs) have attracted interest as protein delivery vehicles due to their biocompatibility and ability to escape the endosomes offering high potential for nuclear delivery. Molecular entry into the nucleus occurs through the nuclear pore complexes, the efficiency of which is dependent on NP size and the presence of nuclear localization sequence (NLS). Chitosan nanoparticles of different sizes (S-NPs ≈ 25 nm; L-NP ≈ 150 nm) were formulated, and they were modified with different densities of the octapeptide NLS CPKKKRKV (S-NPs, 0.25, 0.5, 2.0 NLS/nm(2); L-NPs, 0.6, 0.9, 2 NLS/nm(2)). Unmodified and NLS-tagged NPs were evaluated for their protein loading capacity, extent of cell association, cell uptake, cell surface binding, and finally nuclear delivery efficiency in L929 fibroblasts. To avoid errors generated with cell fractionation and nuclear isolation protocols, nuclear delivery was assessed in intact cells utilizing Förster resonance energy transfer (FRET) fluorometry and microscopy. Although L-NPs showed ≈10-fold increase in protein loading per NP when compared to S-NPs, due to higher cell association and uptake S-NPs showed superior protein delivery. NLS exerts a size and density dependent effect on nanoparticle uptake and surface binding, with a general reduction in NP cell surface binding and an increase in cell uptake with the increase in NLS density (up to 8.4-fold increase in uptake of High-NLS-L-NPs (2 NLS/nm(2)) compared to unmodified L-NPs). However, for nuclear delivery, unmodified S-NPs show higher nuclear localization rates when compared to NLS modified NPs (up to 5-fold by FRET microscopy). For L-NPs an intermediate NLS density (0.9 NLS/nm(2)) seems to provide highest nuclear localization (3.7-fold increase in nuclear delivery compared to High-NLS-L-NPs). Results indicate that a higher NLS density does not result in maximum protein nuclear localization and that a universal optimal density for NPs of different sizes does not exist.

Bowling Green-Warren County Consumer-Career Education Handbook for Exceptional Education. Grades 1-12.

ERIC Educational Resources Information Center

Bowling Green Independent School District, KY.

The document contains consumer and career education curriculum guides for elementary and secondary teachers of handicapped children. Various units are grouped into the following disability categories: primary, intermediate, and secondary educable mentally handicapped; trainable mentally handicapped; emotionally disturbed; physically handicapped;…

Your Rights as Parents of a Handicapped Child.

ERIC Educational Resources Information Center

Crary, James O.; And Others

This booklet provides information on the rights of parents of handicapped children (according to both Illinois and national laws) and describes services required by law for the handicapped child and the necessary steps to obtain these services. Definitions of various mental, physical, and emotional handicaps are given according to legal…

Seven Special Kids: Employment Problems of Handicapped Youth.

ERIC Educational Resources Information Center

Smith, R. C.

A study of the employment problems facing physically and mentally handicapped youth is reported. To illustrate the main points, results of extensive interviews with seven handicapped youth are juxtaposed with statistics and findings. The study looks at tne continuum of services offered to handicapped individuals, including understanding the…

The Canadian Human Rights Act. Physical Handicap and Employment = Loi canadienne sur les droits de la personne. Le handicap physique et l'emploi.

ERIC Educational Resources Information Center

Canadian Human Rights Commission, Ottawa (Ontario).

This booklet presents administrative criteria for implementation of the Canadian Human Rights Act to be used by the Canadian Human Rights Commission to evaluate employment decisions affecting physically handicapped individuals. They are of value to employers who need to know how the Commission will analyze employer arguments that they have not…

An Estimate of the Number of Persons with Visual and Physical Handicaps Eligible for DBPH Services.

ERIC Educational Resources Information Center

Pollet, Dorothy, Comp.

It is estimated that 1,868,000 people are certainly eligible for the services of the Library of Congress Division for the Blind and Physically Handicapped. Between 5,781,500 and 11,384,500 are also possibly eligible. Handicapping conditions which are certainly eligible are severe visual impairment, absence of one or both arms or hands, cerebral…

The feasibility of accommodating physically handicapped individuals on pedestrian over and undercrossing structures. Appendix

DOT National Transportation Integrated Search

2008-09-01

This report describes the evaluation of a sampling of over- and undercrossing structures to identify major and minor access barriers for the physically handicapped. From this evaluation, it was determined that it is feasible to accommodate the physic...

Therapeutic Intervention for the Physically Handicapped

ERIC Educational Resources Information Center

Spillios, James; Janzen, Henry L.

1978-01-01

The need for training counselors specifically for intervention with the physically handicapped is the major focus of this article. Definitions of disabilities, rehabilitation and emotional factors are stressed as important variables in physical and psychotherapeutic treatment. The authors review some of the psychological aspects in counseling the…

Wave instabilities in the presence of non vanishing background in nonlinear Schrödinger systems

PubMed Central

Trillo, S.; Gongora, J. S. Totero; Fratalocchi, A.

2014-01-01

We investigate wave collapse ruled by the generalized nonlinear Schrödinger (NLS) equation in 1+1 dimensions, for localized excitations with non-zero background, establishing through virial identities a new criterion for blow-up. When collapse is arrested, a semiclassical approach allows us to show that the system can favor the formation of dispersive shock waves. The general findings are illustrated with a model of interest to both classical and quantum physics (cubic-quintic NLS equation), demonstrating a radically novel scenario of instability, where solitons identify a marginal condition between blow-up and occurrence of shock waves, triggered by arbitrarily small mass perturbations of different sign. PMID:25468032

The Detroit Approach to Adapted Physical Education and Recreation.

ERIC Educational Resources Information Center

Elkins, Bruce; Czapski, Stephen

The report describes Detroit's Adaptive Physical Education Consortium Project in Michigan. Among the main objectives of the project are to coordinate all physical education and recreation services to the handicapped in the Detroit area; to facilitate the mainstreaming of capable handicapped individuals into existing "regular" physical…

46 CFR 153.440 - Cargo temperature sensors.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Category A NLS or a Category B NLS having a viscosity of at least 25 mPa.s at 20 °C; (ii) A Category C NLS having a viscosity of at least 60 mPa.s at 20 °C; or (iii) A Category A, B, or C NLS that has a melting...

46 CFR 153.440 - Cargo temperature sensors.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Category A NLS or a Category B NLS having a viscosity of at least 25 mPa.s at 20 °C; (ii) A Category C NLS having a viscosity of at least 60 mPa.s at 20 °C; or (iii) A Category A, B, or C NLS that has a melting...

46 CFR 153.440 - Cargo temperature sensors.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Category A NLS or a Category B NLS having a viscosity of at least 25 mPa.s at 20 °C; (ii) A Category C NLS having a viscosity of at least 60 mPa.s at 20 °C; or (iii) A Category A, B, or C NLS that has a melting...

Influence of optical activity on rogue waves propagating in chiral optical fibers.

PubMed

Temgoua, D D Estelle; Kofane, T C

2016-06-01

We derive the nonlinear Schrödinger (NLS) equation in chiral optical fiber with right- and left-hand nonlinear polarization. We use the similarity transformation to reduce the generalized chiral NLS equation to the higher-order integrable Hirota equation. We present the first- and second-order rational solutions of the chiral NLS equation with variable and constant coefficients, based on the modified Darboux transformation method. For some specific set of parameters, the features of chiral optical rogue waves are analyzed from analytical results, showing the influence of optical activity on waves. We also generate the exact solutions of the two-component coupled nonlinear Schrödinger equations, which describe optical activity effects on the propagation of rogue waves, and their properties in linear and nonlinear coupling cases are investigated. The condition of modulation instability of the background reveals the existence of vector rogue waves and the number of stable and unstable branches. Controllability of chiral optical rogue waves is examined by numerical simulations and may bring potential applications in optical fibers and in many other physical systems.

The exact rogue wave recurrence in the NLS periodic setting via matched asymptotic expansions, for 1 and 2 unstable modes

NASA Astrophysics Data System (ADS)

Grinevich, P. G.; Santini, P. M.

2018-04-01

The focusing Nonlinear Schrödinger (NLS) equation is the simplest universal model describing the modulation instability (MI) of quasi monochromatic waves in weakly nonlinear media, the main physical mechanism for the generation of rogue (anomalous) waves (RWs) in Nature. In this paper we investigate the x-periodic Cauchy problem for NLS for a generic periodic initial perturbation of the unstable constant background solution, in the case of N = 1 , 2 unstable modes. We use matched asymptotic expansion techniques to show that the solution of this problem describes an exact deterministic alternate recurrence of linear and nonlinear stages of MI, and that the nonlinear RW stages are described by the N-breather solution of Akhmediev type, whose parameters, different at each RW appearance, are always given in terms of the initial data through elementary functions. This paper is motivated by a preceding work of the authors in which a different approach, the finite gap method, was used to investigate periodic Cauchy problems giving rise to RW recurrence.

The estrogen receptor alpha nuclear localization sequence is critical for fulvestrant-induced degradation of the receptor.

PubMed

Casa, Angelo J; Hochbaum, Daniel; Sreekumar, Sreeja; Oesterreich, Steffi; Lee, Adrian V

2015-11-05

Fulvestrant, a selective estrogen receptor down-regulator (SERD) is a pure competitive antagonist of estrogen receptor alpha (ERα). Fulvestrant binds ERα and reduces the receptor's half-life by increasing protein turnover, however, its mechanism of action is not fully understood. In this study, we show that removal of the ERα nuclear localization sequence (ERΔNLS) resulted in a predominantly cytoplasmic ERα that was degraded in response to 17-β-estradiol (E2) but was resistant to degradation by fulvestrant. ERΔNLS bound the ligands and exhibited receptor interaction similar to ERα, indicating that the lack of degradation was not due to disruption of these processes. Forcing ERΔNLS into the nucleus with a heterologous SV40-NLS did not restore degradation, suggesting that the NLS domain itself, and not merely receptor localization, is critical for fulvestrant-induced ERα degradation. Indeed, cloning of the endogenous ERα NLS onto the N-terminus of ERΔNLS significantly restored both its nuclear localization and turnover in response to fulvestrant. Moreover, mutation of the sumoylation targets K266 and K268 within the NLS impaired fulvestrant-induced ERα degradation. In conclusion, our study provides evidence for the unique role of the ERα NLS in fulvestrant-induced degradation of the receptor. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Science and the Physically Handicapped.

ERIC Educational Resources Information Center

Ricker, Kenneth S.

1979-01-01

The integration of physically (sensory and orthopedic) handicapped students into science classes creates a complex problem for science educators. Suggestions are offered for teacher preparation and for the development of specialized materials and modifications for instructional strategies. (JMF)

26 CFR 1.42-11 - Provision of services.

Code of Federal Regulations, 2010 CFR

2010-04-01

... intermediate care facility for the mentally and physically handicapped. See also § 1.42-9(b). (3) Required... intermediate care facility for the mentally or physically handicapped. For a building described in section 42(i...

26 CFR 1.42-11 - Provision of services.

Code of Federal Regulations, 2013 CFR

2013-04-01

... intermediate care facility for the mentally and physically handicapped. See also § 1.42-9(b). (3) Required... intermediate care facility for the mentally or physically handicapped. For a building described in section 42(i...

Physical Education and Recreation for the Visually Handicapped. Revised Edition.

ERIC Educational Resources Information Center

Buell, Charles E.

Part I of this book on physical education for the visually handicapped deals with what physical educators and recreation specialists should know about blindness. Examples are given of athletic accomplishments of visually impaired or sightless athletes. Prevailing misconceptions and attitudes about blindness are discussed, and the importance of…

Nonlocal nonlinear Schrödinger equations and their soliton solutions

NASA Astrophysics Data System (ADS)

Gürses, Metin; Pekcan, Aslı

2018-05-01

We study standard and nonlocal nonlinear Schrödinger (NLS) equations obtained from the coupled NLS system of equations (Ablowitz-Kaup-Newell-Segur (AKNS) equations) by using standard and nonlocal reductions, respectively. By using the Hirota bilinear method, we first find soliton solutions of the coupled NLS system of equations; then using the reduction formulas, we find the soliton solutions of the standard and nonlocal NLS equations. We give examples for particular values of the parameters and plot the function |q(t, x)|2 for the standard and nonlocal NLS equations.

NLS cycle 1 and NLS 2 base heating technical notes. Appendix 3: Preliminary cycle 1 NLS base heating environments. Cycle 1 NLS base heating environments. NLS 2 650K STME base heating environments

NASA Technical Reports Server (NTRS)

Bender, Robert L.; Reardon, John E.; Prendergast, Maurice J.; Schmitz, Craig P.; Brown, John R.

1992-01-01

A preliminary analysis of National Launch System ascent plume induced base heating environments has been completed to support the Induced Environments Panel's objective to assist in maturing the NLS vehicle (1.5 stage and heavy launch lift vehicle) design. Environments during ascent have been determined from this analysis for a few selected locations on the engine nozzles and base heat shield for both vehicles. The environments reflect early summer 1991 configurations and performance data and conservative methodology. A more complete and thorough analysis is under way to update these environments for the cycle 1 review in January 1992.

45 CFR 84.37 - Nonacademic services.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) Nonacademic and extracurricular services and activities may include counseling services, physical recreational... recipient may offer to handicapped students physical education and athletic activities that are separate or... HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Preschool, Elementary, and...

Screening for Physical Problems in Classrooms for Severely Handicapped Students.

ERIC Educational Resources Information Center

Dever, Richard; Knapczyk, Dennis

1980-01-01

The authors present a screening device with which teachers of severely handicapped students may detect the presence of a physical problem. The screening approach covers vision, auditory problems, seizures, orthopedic problems, and pain. (CL)

46 CFR 153.486 - Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D.

Code of Federal Regulations, 2014 CFR

2014-10-01

... COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.486 Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D. (a) If NLS... 46 Shipping 5 2014-10-01 2014-10-01 false Design and equipment for removing NLS residue by...

46 CFR 153.486 - Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D.

Code of Federal Regulations, 2013 CFR

2013-10-01

... COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.486 Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D. (a) If NLS... 46 Shipping 5 2013-10-01 2013-10-01 false Design and equipment for removing NLS residue by...

46 CFR 153.486 - Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D.

Code of Federal Regulations, 2012 CFR

2012-10-01

... COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.486 Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D. (a) If NLS... 46 Shipping 5 2012-10-01 2012-10-01 false Design and equipment for removing NLS residue by...

46 CFR 153.486 - Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D.

Code of Federal Regulations, 2010 CFR

2010-10-01

... COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.486 Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D. (a) If NLS... 46 Shipping 5 2010-10-01 2010-10-01 false Design and equipment for removing NLS residue by...

46 CFR 153.486 - Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D.

Code of Federal Regulations, 2011 CFR

2011-10-01

... COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.486 Design and equipment for removing NLS residue by ventilation: Categories A, B, C, and D. (a) If NLS... 46 Shipping 5 2011-10-01 2011-10-01 false Design and equipment for removing NLS residue by...

Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae.

PubMed

Wei, Tong; Chen, Tsung-Chi; Ho, Yuen Ting; Ronald, Pamela C

2016-01-01

The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae ( Xoo ). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag ( Ubi -XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. To determine if the predicted NLS is required for XA21-mediated immunity in planta , we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions ( Ubi -XA21nls-GFP). Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higher Xoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed in Ubi- XA21-GFP. These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.

Narrow vs. Broad line Seyfert 1 galaxies: X-ray, optical and mid-infrared AGN characteristics

NASA Astrophysics Data System (ADS)

Lakićević, Maša; Popović, Luka Č.; Kovačević-Dojčinović, Jelena

2018-05-01

We investigated narrow line Seyfert 1 galaxies (NLS1s) at optical, mid-infrared (MIR) and X-ray wavelengths, comparing them to the broad line active galactic nuclei (BLAGNs). We found that black hole mass, coronal line luminosities, X-ray hardness ratio and X-ray, optical and MIR luminosities are higher for the BLAGNs than for NLS1s, while policyclic aromatic hydrocarbon (PAH) contribution and the accretion rates are higher for the NLS1s. Furthermore, we found some trends among spectral parameters that NLS1s have and BLAGNs do not have. The evolution of FWHM(Hβ) with the luminosities of MIR and coronal lines, continuum luminosities, PAH contribution, Hβ broad line luminosity, FWHM[O III] and EW(HβNLR), are important trends found for NLS1s. That may contribute to the insight that NLS1s are developing AGNs, growing their black holes, while their luminosities and FWHM(Hβ) consequently grow, and that BLAGNs are mature, larger objects of slower and/or different evolution. Black hole mass is related to PAH contribution only for NLS1s, which may suggest that PAHs are more efficiently destroyed in NLS1s.

JET PROPERTIES OF GeV-SELECTED RADIO-LOUD NARROW-LINE SEYFERT 1 GALAXIES AND POSSIBLE CONNECTION TO THEIR DISK AND CORONA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, Xiao-Na; Lin, Da-Bin; Liang, En-Wei

The observed spectral energy distributions of five GeV-selected narrow-line Seyfert 1 (NLS1) galaxies are fitted with a model including the radiation ingredients from the relativistic jet, the accretion disk, and the corona. We compare the properties of these GeV NLS1 galaxies with flat spectrum radio quasars (FSRQs), BL Lacertae objects (BL Lacs), and radio-quiet (RQ) Seyfert galaxies, and explore possible hints for jet-disk/corona connection. Our results show that the radiation physics and the jet properties of the GeV NLS1 galaxies resemble that of FSRQs. The luminosity variations of PMN J0948+0022 and 1H 0323+342 at the GeV band is tightly correlatedmore » with the beaming factor (δ), similar to that observed in FSRQ 3C 279. The accretion disk luminosities and the jet powers of the GeV NLS1 galaxies cover both the ranges of FSRQs and BL Lacs. With the detection of bright corona emission in 1H 0323+342, we show that the ratio of the corona luminosity (L {sub corona}) to the accretion disk luminosity (L {sub d}) is marginally within the high end of this ratio distribution for an RQ Seyfert galaxy sample, and the variation of jet luminosity may connect with L {sub corona}. However, it is still unclear whether a system with a high L {sub corona}/L {sub d} ratio prefers to power a jet.« less

Physical Education Facilities for the Handicapped.

ERIC Educational Resources Information Center

Isaacs, Larry; Frederick, Stephen D.

1980-01-01

Physical education facilities at Wright State University in Dayton, Ohio have been adapted for the recreational needs of handicapped students. Changes include a special exercise room, accessible locker and shower facilities, a pool area, and a wheelchair repair shop. (CJ)

Does the Open Door Include the Physically Handicapped?

ERIC Educational Resources Information Center

Tuscher, James; Fox, Gary C.

1971-01-01

Though the community college offers many advantages to the physically handicapped because of its diversified programs and extensive counseling services, a recent survey found that too few community colleges provide the necessary architectural modifications to make them accessible. (NF)

Identification and functional characterization of a novel bipartite nuclear localization sequence in ARID1A

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bateman, Nicholas W.; The John P. Murtha Cancer Center, Walter Reed National Military Medical Center, 8901 Wisconsin Avenue, Bethesda 20889, MD; Shoji, Yutaka

2016-01-01

AT-rich interactive domain-containing protein 1A (ARID1A) is a recently identified nuclear tumor suppressor frequently altered in solid tumor malignancies. We have identified a bipartite-like nuclear localization sequence (NLS) that contributes to nuclear import of ARID1A not previously described. We functionally confirm activity using GFP constructs fused with wild-type or mutant NLS sequences. We further show that cyto-nuclear localized, bipartite NLS mutant ARID1A exhibits greater stability than nuclear-localized, wild-type ARID1A. Identification of this undescribed functional NLS within ARID1A contributes vital insights to rationalize the impact of ARID1A missense mutations observed in patient tumors. - Highlights: • We have identified a bipartitemore » nuclear localization sequence (NLS) in ARID1A. • Confirmation of the NLS was performed using GFP constructs. • NLS mutant ARID1A exhibits greater stability than wild-type ARID1A.« less

A Data Based Gymnasium: A Systematic Approach to Physical Education for the Handicapped.

ERIC Educational Resources Information Center

Dunn, John M.; And Others

The authors describe a data based physical education curriculum designed for low incidence severely handicapped students by Oregon State University in conjunction with Teaching Research. Chapter 1 provides a brief introduction to the physical education curriculum and the Teaching Research model with emphasis placed on the importance of…

Data Based Physical Education for the Severely Handicapped.

ERIC Educational Resources Information Center

Dunn, John M.; Morehouse, Jim W.

The paper provides an overview of a data based physical education program for the severely handicapped which has been developed at Oregon State University's Department of Physical Education in cooperation with the Special Education Department of Teaching Research. Concepts which form the basis of the model include that there is no way of…

The Physically Handicapped and Health Impaired Children: Do They Prosper in Regular Toronto Elementary Schools?

ERIC Educational Resources Information Center

Larter, Sylvia E.

The experience of 88 physically handicapped and health impaired (PH/HI) children attending Toronto, Canada, regular elementary schools in either regular classes or "integrated" special education classes was assessed with regard to their academic, social, emotional, medical, and physical needs. The integrated classification meant they…

The Due-Able Process Could Happen to You! Physical Educators, Handicapped Students, and the Law.

ERIC Educational Resources Information Center

Kennedy, Susan O.; And Others

1989-01-01

This article presents basic information for regular and special physical educators to help them better understand the procedural rights of parents as well as the schools, and to help them make appropriate judgments for the physical education placement and programing of students with handicaps. (IAH)

Integrating Physically Handicapped Pupils.

ERIC Educational Resources Information Center

Hodgson, Ann

1984-01-01

Intended for regular class teachers working with mainstreamed physically handicapped pupils, the article offers guidelines regarding school organization, conditions directly affecting the pupil, and classroom considerations. A brief review of the major conditions (such as allergies, cerebral palsy, and muscular dystrophy) is presented. (CL)

Cassette Books.

ERIC Educational Resources Information Center

Library of Congress, Washington, DC. National Library Service for the Blind and Physically Handicapped.

This catalog lists cassette books produced by the National Library Service for the Blind and Physically Handicapped during 1989. Books are listed alphabetically within subject categories under nonfiction and fiction headings. Nonfiction categories include: animals and wildlife, the arts, bestsellers, biography, blindness and physical handicaps,…

14 CFR 1251.201 - Reasonable accommodation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... OF HANDICAP Employment Practices § 1251.201 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an otherwise qualified handicapped... facilities used by employees readily accessible to and usable by handicapped persons; and (2) Job...

45 CFR 84.12 - Reasonable accommodation.

Code of Federal Regulations, 2012 CFR

2012-10-01

... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices... physical or mental limitations of an otherwise qualified handicapped applicant or employee unless the... readily accessible to and usable by handicapped persons, and (2) job restructuring, part-time or modified...

14 CFR 1251.201 - Reasonable accommodation.

Code of Federal Regulations, 2012 CFR

2012-01-01

... OF HANDICAP Employment Practices § 1251.201 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an otherwise qualified handicapped... facilities used by employees readily accessible to and usable by handicapped persons; and (2) Job...

14 CFR 1251.201 - Reasonable accommodation.

Code of Federal Regulations, 2013 CFR

2013-01-01

... OF HANDICAP Employment Practices § 1251.201 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an otherwise qualified handicapped... facilities used by employees readily accessible to and usable by handicapped persons; and (2) Job...

14 CFR 1251.201 - Reasonable accommodation.

Code of Federal Regulations, 2011 CFR

2011-01-01

... OF HANDICAP Employment Practices § 1251.201 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an otherwise qualified handicapped... facilities used by employees readily accessible to and usable by handicapped persons; and (2) Job...

45 CFR 1151.32 - Reasonable accommodation.

Code of Federal Regulations, 2012 CFR

2012-10-01

... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NONDISCRIMINATION ON THE BASIS OF HANDICAP Discrimination... to the known physical or mental limitations of an otherwise qualified handicapped applicant or... by employees readily accessible to and usable by handicapped persons; and (2) Job restructuring, part...

45 CFR 1151.32 - Reasonable accommodation.

Code of Federal Regulations, 2014 CFR

2014-10-01

... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NONDISCRIMINATION ON THE BASIS OF HANDICAP Discrimination... to the known physical or mental limitations of an otherwise qualified handicapped applicant or... by employees readily accessible to and usable by handicapped persons; and (2) Job restructuring, part...

45 CFR 1151.32 - Reasonable accommodation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NONDISCRIMINATION ON THE BASIS OF HANDICAP Discrimination... to the known physical or mental limitations of an otherwise qualified handicapped applicant or... by employees readily accessible to and usable by handicapped persons; and (2) Job restructuring, part...

45 CFR 1151.32 - Reasonable accommodation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NONDISCRIMINATION ON THE BASIS OF HANDICAP Discrimination... to the known physical or mental limitations of an otherwise qualified handicapped applicant or... by employees readily accessible to and usable by handicapped persons; and (2) Job restructuring, part...

45 CFR 1151.32 - Reasonable accommodation.

Code of Federal Regulations, 2013 CFR

2013-10-01

... HUMANITIES NATIONAL ENDOWMENT FOR THE ARTS NONDISCRIMINATION ON THE BASIS OF HANDICAP Discrimination... to the known physical or mental limitations of an otherwise qualified handicapped applicant or... by employees readily accessible to and usable by handicapped persons; and (2) Job restructuring, part...

Portrayal of Physically Handicapped Characters in Adolescent Fiction.

ERIC Educational Resources Information Center

Stroud, Janet G.

1980-01-01

Reviews the portrayals of handicapped fictional characters for prognosis of the handicap, for effect on the subject and other characters, and for treatment of the disability. Twelve recently published books are examined for their readership interest for young people. (RAA)

Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro

PubMed Central

Medina-Alarcón, Kaila P.; Singulani, Junya L.; Voltan, Aline R.; Sardi, Janaina C. O.; Petrônio, Maicon S.; Santos, Mariana B.; Polaquini, Carlos R.; Regasini, Luis O.; Bolzani, Vanderlan S.; da Silva, Dulce H. S.; Chorilli, Marlus; Mendes-Giannini, Maria J. S.; Fusco-Almeida, Ana M.

2017-01-01

Dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. Nanostructured lipid systems (NLS) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. This work aimed to evaluate dodecyl protocatechuate loaded into a NLS (NLS+dodecyl) as a strategy for the treatment of Paracoccidioides brasiliensis and P. lutzii in vitro. Antifungal activity against P. brasiliensis and P. lutzii was evaluated using the microdilution technique. NLS+dodecyl showed high antifungal activity with a minimum inhibitory concentration ranging from 0.06 to 0.03 μg/mL; 4- to 16-fold higher than that of free dodecyl. NLS+dodecyl was able to inhibit fungal adhesion of the extracellular artificial matrix proteins (laminin and fibronectin), resulting in 82.4 and 81% inhibition, respectively, an increase of 8–17% compared with free dodecyl. These findings corroborate previous results demonstrating 65 and 74% inhibition of fungal adhesion in pulmonary fibroblast cells by dodecyl and NLS+dodecyl, respectively, representing a 9% increase in inhibition for NLS+dodecyl. Subsequently, cytotoxicity was evaluated using the 0.4% sulforhodamine B assay. NLS+dodecyl did not exhibit cytotoxicity in MRC5 (human pneumocyte) and HepG2 (human hepatic carcinoma) cells, thus increasing the selectivity index for NLS+dodecyl. In addition, cytotoxicity was evaluated in vivo using the Caenorhabditis elegans model; neither dodecyl nor NLS+dodecyl exhibited any toxic effects. Taken together, these results suggest that NLS can be used as a strategy to improve the activity of dodecyl against P. brasiliensis and P. lutzii because it improves antifungal activity, increases the inhibition of fungal adhesion in lung cells and the extracellular matrix in vitro, and does not exhibit any toxicity both in vitro and in vivo. PMID:28659880

Stress-related behaviors among horses used in a therapeutic riding program.

PubMed

Kaiser, Lana; Heleski, Camie R; Siegford, Janice; Smith, Katharine Ann

2006-01-01

To determine whether therapeutic riding resulted in higher levels of stress or frustration for horses than did recreational riding and whether therapeutic riding with at-risk individuals was more stressful for the horses than was therapeutic riding with individuals with physical or emotional handicaps. Observational study. 14 horses in a therapeutic riding program. An ethogram of equine behaviors was created, and horses were observed while ridden by 5 groups of riders (recreational riders, physically handicapped riders, psychologically handicapped riders, at risk children, and special education children). Number of stress-related behaviors (ears pinned back, head raised, head turned, head tossed, head shaken, head down, and defecation) was compared among groups. No significant differences in mean number of stress-related behaviors were found when horses were ridden by recreational riders, physically handicapped riders, psychologically handicapped riders, or special education children. However, mean number of stress-related behaviors was significantly higher when horses were ridden by the at-risk children. Results suggest that for horses in a therapeutic riding program, being ridden by physically or psychologically handicapped individuals is no more stressful for the horses than is being ridden in the same setting by recreational riders. However, at-risk children caused more stress to the horses, suggesting that the time horses are ridden by at-risk children should be limited both daily and weekly.

Structural basis for the regulation of nuclear import of Epstein-Barr virus nuclear antigen 1 (EBNA1) by phosphorylation of the nuclear localization signal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nakada, Ryohei; Hirano, Hidemi; Structural Biology Research Center, Graduate School of Science, Nagoya University

Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is expressed in every EBV-positive tumor and is essential for the maintenance, replication, and transcription of the EBV genome in the nucleus of host cells. EBNA1 is a serine phosphoprotein, and it has been shown that phosphorylation of S385 in the nuclear localization signal (NLS) of EBNA1 increases the binding affinity to the nuclear import adaptor importin-α1 as well as importin-α5, and stimulates nuclear import of EBNA1. To gain insights into how phosphorylation of the EBNA1 NLS regulates nuclear import, we have determined the crystal structures of two peptide complexes of importin-α1: onemore » with S385-phosphorylated EBNA1 NLS peptide, determined at 2.0 Å resolution, and one with non-phosphorylated EBNA1 NLS peptide, determined at 2.2 Å resolution. The structures show that EBNA1 NLS binds to the major and minor NLS-binding sites of importin-α1, and indicate that the binding affinity of the EBNA1 NLS to the minor NLS-binding site could be enhanced by phosphorylation of S385 through electrostatic interaction between the phosphate group of phospho-S385 and K392 of importin-α1 (corresponding to R395 of importin-α5) on armadillo repeat 8. - Highlights: • Nuclear import of EBNA1 can be regulated by phosphorylation of NLS. • Crystal structures of importin-α1 bound to the NLS peptides of EBNA1 are solved. • Structures provide insights into how phosphorylation can regulate nuclear import.« less

Large-scale environments of narrow-line Seyfert 1 galaxies

NASA Astrophysics Data System (ADS)

Järvelä, E.; Lähteenmäki, A.; Lietzen, H.; Poudel, A.; Heinämäki, P.; Einasto, M.

2017-09-01

Studying large-scale environments of narrow-line Seyfert 1 (NLS1) galaxies gives a new perspective on their properties, particularly their radio loudness. The large-scale environment is believed to have an impact on the evolution and intrinsic properties of galaxies, however, NLS1 sources have not been studied in this context before. We have a large and diverse sample of 1341 NLS1 galaxies and three separate environment data sets constructed using Sloan Digital Sky Survey. We use various statistical methods to investigate how the properties of NLS1 galaxies are connected to the large-scale environment, and compare the large-scale environments of NLS1 galaxies with other active galactic nuclei (AGN) classes, for example, other jetted AGN and broad-line Seyfert 1 (BLS1) galaxies, to study how they are related. NLS1 galaxies reside in less dense environments than any of the comparison samples, thus confirming their young age. The average large-scale environment density and environmental distribution of NLS1 sources is clearly different compared to BLS1 galaxies, thus it is improbable that they could be the parent population of NLS1 galaxies and unified by orientation. Within the NLS1 class there is a trend of increasing radio loudness with increasing large-scale environment density, indicating that the large-scale environment affects their intrinsic properties. Our results suggest that the NLS1 class of sources is not homogeneous, and furthermore, that a considerable fraction of them are misclassified. We further support a published proposal to replace the traditional classification to radio-loud, and radio-quiet or radio-silent sources with a division into jetted and non-jetted sources.

A Multigrid NLS-4DVar Data Assimilation Scheme with Advanced Research WRF (ARW)

NASA Astrophysics Data System (ADS)

Zhang, H.; Tian, X.

2017-12-01

The motions of the atmosphere have multiscale properties in space and/or time, and the background error covariance matrix (Β) should thus contain error information at different correlation scales. To obtain an optimal analysis, the multigrid three-dimensional variational data assimilation scheme is used widely when sequentially correcting errors from large to small scales. However, introduction of the multigrid technique into four-dimensional variational data assimilation is not easy, due to its strong dependence on the adjoint model, which has extremely high computational costs in data coding, maintenance, and updating. In this study, the multigrid technique was introduced into the nonlinear least-squares four-dimensional variational assimilation (NLS-4DVar) method, which is an advanced four-dimensional ensemble-variational method that can be applied without invoking the adjoint models. The multigrid NLS-4DVar (MG-NLS-4DVar) scheme uses the number of grid points to control the scale, with doubling of this number when moving from a coarse to a finer grid. Furthermore, the MG-NLS-4DVar scheme not only retains the advantages of NLS-4DVar, but also sufficiently corrects multiscale errors to achieve a highly accurate analysis. The effectiveness and efficiency of the proposed MG-NLS-4DVar scheme were evaluated by several groups of observing system simulation experiments using the Advanced Research Weather Research and Forecasting Model. MG-NLS-4DVar outperformed NLS-4DVar, with a lower computational cost.

Physical Education Programming for Exceptional Learners.

ERIC Educational Resources Information Center

Folio, M. Rhonda

This book provides programming ideas, methods, strategies, and adaptations of the learning environment for implementing physical education programs for handicapped students. Part I, "Legislation and the Challenge," introduces Public Law 94-142, the Education for All Handicapped Children Act, its mandates, and its procedures for…

The feasibility of accommodating physically handicapped individuals on pedestrian over and undercrossing structures

DOT National Transportation Integrated Search

1980-09-01

The objective of this study was to determine the feasibility of accommodating the physically handicapped on over- and undercrossing structures. Based upon the evaluation of 124 crossing structures, 86 percent of these structures had at least one majo...

22 CFR 142.47 - Non-academic services.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS... not discriminate on the basis of handicap. A recipient that offers physical education courses or that operates or sponsors intercollegiate, club, or intramural athletics shall provide to qualified handicapped...

22 CFR 142.47 - Non-academic services.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS... not discriminate on the basis of handicap. A recipient that offers physical education courses or that operates or sponsors intercollegiate, club, or intramural athletics shall provide to qualified handicapped...

22 CFR 142.47 - Non-academic services.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS... not discriminate on the basis of handicap. A recipient that offers physical education courses or that operates or sponsors intercollegiate, club, or intramural athletics shall provide to qualified handicapped...

22 CFR 142.47 - Non-academic services.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS... not discriminate on the basis of handicap. A recipient that offers physical education courses or that operates or sponsors intercollegiate, club, or intramural athletics shall provide to qualified handicapped...

Preschool Curriculum for Exceptional Children.

ERIC Educational Resources Information Center

Kelley-Saur, Cheryl

The curriculum guide for use with preschool handicapped children provides 28 activity units as well as general teaching suggestions. An initial section covers ideas for adapting activities to specific handicapping conditions including the visually impaired, hearing impaired, physically handicapped, and mentally disabled. Subsections also cover…

Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae

DOE PAGES

Wei, Tong; Chen, Tsung-Chi; Ho, Yuen Ting; ...

2016-10-05

Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae( Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag ( Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunity in planta, we generated transgenic plants overexpressing an XA21 variant carrying themore » NLS with the same alanine substitutions ( Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higher Xoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed in Ubi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.« less

Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wei, Tong; Chen, Tsung-Chi; Ho, Yuen Ting

Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae( Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag ( Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunity in planta, we generated transgenic plants overexpressing an XA21 variant carrying themore » NLS with the same alanine substitutions ( Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higher Xoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed in Ubi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.« less

Crystal Structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2

PubMed Central

Soniat, Michael; Sampathkumar, Parthasarathy; Collett, Garen; Gizzi, Anthony S.; Banu, Radhika N.; Bhosle, Rahul C.; Chamala, Swetha; Chowdhury, Sukanya; Fiser, Andras; Glenn, Alan S.; Hammonds, James; Hillerich, Brandan; Khafizov, Kamil; Love, James D.; Matikainen, Bridget; Seidel, Ronald D.; Toro, Rafael; Kumar, P. Rajesh; Bonanno, Jeffery B.; Chook, Yuh Min; Almo, Steven C.

2013-01-01

Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2•PY-NLS structures. PMID:23535894

Fun Food Experiences for Preschool Children with Handicaps.

ERIC Educational Resources Information Center

Mosiman, Joyce; And Others

The manual includes lesson plans and suggestions for introducting handicapped children to good nutrition through enjoyable activities. Special considerations for specific types of handicaps are briefly noted (including safety considerations for children with physical disabilities). Each lesson is organized according to purpose, teacher objectives,…

Exceptional Pupils. Special Education Bulletin Number 1.

ERIC Educational Resources Information Center

Indiana State Dept. of Public Instruction, Indianapolis. Div. of Special Education.

An introduction to exceptional children precedes a discussion of each of the following areas of exceptionality; giftedness, mental retardation, physical handicaps and special health problems, blindness and partial vision, aural handicaps, speech handicaps, emotional disturbance, and learning disabilities. Each chapter is followed by a bibliography…

41 CFR 101-8.311 - Historic Preservation Programs.

Code of Federal Regulations, 2011 CFR

2011-07-01

... FEDERAL FINANCIAL ASSISTANCE 8.3-Discrimination Prohibited on the Basis of Handicap § 101-8.311 Historic... and usable by handicapped persons. This paragraph does not necessarily require a recipient to make... by handicapped persons. Methods of achieving accessibility include: (i) Making physical alterations...

41 CFR 101-8.311 - Historic Preservation Programs.

Code of Federal Regulations, 2014 CFR

2014-07-01

... FEDERAL FINANCIAL ASSISTANCE 8.3-Discrimination Prohibited on the Basis of Handicap § 101-8.311 Historic... and usable by handicapped persons. This paragraph does not necessarily require a recipient to make... by handicapped persons. Methods of achieving accessibility include: (i) Making physical alterations...

41 CFR 101-8.311 - Historic Preservation Programs.

Code of Federal Regulations, 2013 CFR

2013-07-01

... FEDERAL FINANCIAL ASSISTANCE 8.3-Discrimination Prohibited on the Basis of Handicap § 101-8.311 Historic... and usable by handicapped persons. This paragraph does not necessarily require a recipient to make... by handicapped persons. Methods of achieving accessibility include: (i) Making physical alterations...

41 CFR 101-8.311 - Historic Preservation Programs.

Code of Federal Regulations, 2012 CFR

2012-07-01

... FEDERAL FINANCIAL ASSISTANCE 8.3-Discrimination Prohibited on the Basis of Handicap § 101-8.311 Historic... and usable by handicapped persons. This paragraph does not necessarily require a recipient to make... by handicapped persons. Methods of achieving accessibility include: (i) Making physical alterations...

Aquatics for the Handicapped--A Review of Literature.

ERIC Educational Resources Information Center

Christie, Irene

1985-01-01

The author reviews the literature on aquatic activity for the disabled, discussing the physical, physiological, psychological, and sociological benefits of swimming and water safety activities. Unique properties of water and legal requirements regarding physical education of the handicapped, specifically citing the development of skills in…

Principles and Methods of Adapted Physical Education.

ERIC Educational Resources Information Center

Arnheim, Daniel D.; And Others

Programs in adapted physical education are presented preceded by a background of services for the handicapped, by the psychosocial implications of disability, and by the growth and development of the handicapped. Elements of conducting programs discussed are organization and administration, class organization, facilities, exercise programs…

32 CFR 56.3 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... more major life activities. (4) Is regarded as having an impairment. Has: (i) A physical or mental... ON THE BASIS OF HANDICAP IN PROGRAMS AND ACTIVITIES ASSISTED OR CONDUCTED BY THE DEPARTMENT OF... Federal government. (c) Handicapped person. Any person who has a physical or mental impairment that...

Comprehensive Microcomputer Applications for Severely Physically Handicapped Children.

ERIC Educational Resources Information Center

Rushakoff, G. Evan; Lombardino, Linda J.

1983-01-01

Explained for educators of severly physically handicapped (SPH) children are basic component parts of the microcomputer system, adaptations for children unable to use a standard keyboard, and applications for communication, academic work, writing, creative arts, recreation, future employment, and young SPH children. Factors educators should…

Vapor deposition on doublet airfoil substrates: Control of coating thickness and microstructure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rodgers, Theron M.; Zhao, Hengbei; Wadley, Haydn N. G., E-mail: haydn@virginia.edu

Gas jet assisted vapor deposition processes for depositing coatings are conducted at higher pressures than conventional physical vapor deposition methods, and have shown promise for coating complex shaped substrates including those with non-line-of-sight (NLS) regions on their surface. These regions typically receive vapor atoms at a lower rate and with a wider incident angular distribution than substrate regions in line-of-sight (LS) of the vapor source. To investigate the coating of such substrates, the thickness and microstructure variation along the inner (curved) surfaces of a model doublet airfoil containing both LS and NLS regions has been investigated. Results from atomistic simulationsmore » and experiments confirm that the coating's thickness is thinner in flux-shadowed regions than in other regions for all the coating processes investigated. They also indicated that the coatings columnar microstructure and pore volume fraction vary with surface location through the LS to NLS transition zone. A substrate rotation strategy for optimizing the thickness over the entire doublet airfoil surface was investigated, and led to the identification of a process that resulted in only small variation of coating thickness, columnar growth angle, and pore volume fraction on all doublet airfoil surfaces.« less

Swimming for the Handicapped Child and Adult: Occasional Papers No. 10.

ERIC Educational Resources Information Center

Neishloss, Lou

Outlined are physiological and psychological values of swimming for the handicapped, basic principles and teaching procedures for instructing physically handicapped persons, and specific suggestions for teaching swimming to persons with the following conditions; amputations, polio, paraplegia, cerebral palsy, spina bifida, Legg-Perthes Disease,…

22 CFR 142.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR... Respect to the Handicapped in Federally-Assisted Programs,” issued April 28, 1976. (b) The Act means the... property. (j) Handicapped person. (1) means any person who: (i) has a physical or mental impairment which...

22 CFR 142.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR... Respect to the Handicapped in Federally-Assisted Programs,” issued April 28, 1976. (b) The Act means the... property. (j) Handicapped person. (1) means any person who: (i) has a physical or mental impairment which...

22 CFR 142.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR... Respect to the Handicapped in Federally-Assisted Programs,” issued April 28, 1976. (b) The Act means the... property. (j) Handicapped person. (1) means any person who: (i) has a physical or mental impairment which...

Proceedings from Statewide Conference on Physical Education for Handicapped Children and Youth (1st, Ithaca, New York, October 1-3, 1972).

ERIC Educational Resources Information Center

Ithaca Coll., NY.

The conference attempted to improve experiences in physical education and recreation for all children, regardless of handicap, through the preparation and demonstration of model instructional units. The 38 units reported are in the areas of perceptual-motor development, physical fitness, aquatics, basic conditioning exercises for gymnastics,…

Sequenced Instructional Programs in Physical Education for the Handicapped.

ERIC Educational Resources Information Center

Carr, Dorothy B.; And Others

The curriculum guidelines for a comprehensive physical education program consist of developmentally sequenced skills and instructional activities appropriate for handicapped children from early preschool age (18 months) through high school. Suggested activities and materials are arranged in color-coded sections on motor and movement skills,…

7 CFR 1753.25 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) (Access to Physically Handicapped), which requires certain buildings financed with Federal funds be designed and constructed to be accessible to the physically handicapped. (2) Pub. L. 91-596 (29 U.S.C. 651.... Separate contracts may be used for planting shrubbery, surfacing of roads and parking areas, and other...

7 CFR 1753.25 - General.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) (Access to Physically Handicapped), which requires certain buildings financed with Federal funds be designed and constructed to be accessible to the physically handicapped. (2) Pub. L. 91-596 (29 U.S.C. 651.... Separate contracts may be used for planting shrubbery, surfacing of roads and parking areas, and other...

7 CFR 1753.25 - General.

Code of Federal Regulations, 2013 CFR

2013-01-01

...) (Access to Physically Handicapped), which requires certain buildings financed with Federal funds be designed and constructed to be accessible to the physically handicapped. (2) Pub. L. 91-596 (29 U.S.C. 651.... Separate contracts may be used for planting shrubbery, surfacing of roads and parking areas, and other...

7 CFR 1753.25 - General.

Code of Federal Regulations, 2012 CFR

2012-01-01

...) (Access to Physically Handicapped), which requires certain buildings financed with Federal funds be designed and constructed to be accessible to the physically handicapped. (2) Pub. L. 91-596 (29 U.S.C. 651.... Separate contracts may be used for planting shrubbery, surfacing of roads and parking areas, and other...

7 CFR 1753.25 - General.

Code of Federal Regulations, 2014 CFR

2014-01-01

...) (Access to Physically Handicapped), which requires certain buildings financed with Federal funds be designed and constructed to be accessible to the physically handicapped. (2) Pub. L. 91-596 (29 U.S.C. 651.... Separate contracts may be used for planting shrubbery, surfacing of roads and parking areas, and other...

Children's Physical Appearance and Adult Expectations.

ERIC Educational Resources Information Center

Feeg, Veronica DeCarolis; Peters, Donald L.

Caregivers' expectancies and behaviors in relationship to young handicapped children were investigated in this paper. Two studies were conducted in an attempt to answer three questions: (1) How will caregivers respond to the young handicapped child? (2) Do the child's stimulus characteristics, such as physical appearance, create biases in…

36 CFR 910.34 - Accommodations for the physically handicapped.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Accommodations for the physically handicapped. 910.34 Section 910.34 Parks, Forests, and Public Property PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION GENERAL GUIDELINES AND UNIFORM STANDARDS FOR URBAN PLANNING AND DESIGN OF DEVELOPMENT...

36 CFR 910.34 - Accommodations for the physically handicapped.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Accommodations for the physically handicapped. 910.34 Section 910.34 Parks, Forests, and Public Property PENNSYLVANIA AVENUE DEVELOPMENT CORPORATION GENERAL GUIDELINES AND UNIFORM STANDARDS FOR URBAN PLANNING AND DESIGN OF DEVELOPMENT...

Sensational Studies in Marine Science.

ERIC Educational Resources Information Center

Keller, E. C., Jr.; Schroyer, Fred C.

1981-01-01

Presents a description of a five-week summer course in marine biology and oceanography offered to college-bound, secondary students with varied physical handicaps. Summarizes insights gained after four summer sessions related to communication problems, physical arrangements for the wheelchair-bound, and handicap-proof maps; evaluates the course's…

[22q11.2 deletion: handicap-related problems and coping strategies of primary caregivers].

PubMed

Briegel, Wolfgang; Schneider, Marco; Schwab, K Otfried

2009-11-01

To investigate handicap-related problems of children and adolescents with 22q11.2 deletion syndrome and their primary caregivers' coping strategies. Primary caregivers of 153 subjects aged 2-16 years were anonymously asked to fill out questionnaires, e.g., the Handicap Related Problems for Parents Inventory. Primary caregivers of 96 subjects (53 males, 43 females; mean age: 7;0 [2;1-16;11] years) sent back questionnaires. Patient's behaviour and discipline were the most important handicap-related problems. Significant correlations could be found between the patient's age and his/her relationship with the primary caregiver (rho=0.228; p=.029) and other family members (rho=0.293; p=.004). Compared to other parents of physically handicapped children or those with multiple handicaps, these parents did not experience increased stress. The more the coping strategies "self-fulfillment" and "intensification of partnership" were used, the lower parental stress was (p=.012, p=.025, respectively). "Focusing on the handicapped child" was positively correlated with high parental stress (p=.000). With regard to parental stress and coping strategies, primary caregivers of children and adolescents with 22q11.2 deletion do not significantly differ from other parents of physically handicapped children. As handicap-related family problems increase with the patient's age, a growing need for counseling, especially for aspects of parenting and discipline, and for treatment can be presumed.

46 CFR 153.1128 - Discharge of NLS residue from a cargo tank to the sea: Categories A. B, C, and D.

Code of Federal Regulations, 2010 CFR

2010-10-01

... the ship must be in water at least 25 m (76.2 ft) deep: (1) Category B or C NLS residue diluted to...) To discharge a Category D NLS residue to which 10 times its volume in water is added and mixed, the...) In water at least 25 m (76.2 ft) deep; (2) If discharging the residue of a Category A NLS cargo...

Rights Handbook for Handicapped Children and Adults.

ERIC Educational Resources Information Center

Des Jardins, Charlotte; Hull, Rita

The handbook is intended as a reference to rights of handicapped children and adults as defined by federal and state legislation. A section on education makes up a major portion of the document and includes information on the following areas: definition of handicapped children, early childhood, visual impairment, hearing impairment, physical or…

Self-Handicapping in School Physical Education: The Influence of the Motivational Climate

ERIC Educational Resources Information Center

Standage, Martyn; Treasure, Darren C.; Hooper, Katherine; Kuczka, Kendy

2007-01-01

Background: Self-handicapping is an attribution-related process whereby individuals create performance impediments/excuses to protect self-worth in socially evaluative environments. Thus, the prevailing motivational climate would appear to be an important factor when attempting to understand the situational self-handicapping process within school…

A Computerized Bibliographic Service for the Blind and Physically Handicapped

ERIC Educational Resources Information Center

Friedman, Morton H.

1975-01-01

Describes a three-year plan and a system study designed to produce a computerized union catalog and an in-process file for both the Library of Congress Division for the Blind and Physically Handicapped and a network of almost 200 libraries throughout the nation. (Author/PF)

Outdoor Recreation for the Physically Handicapped. A Handbook of Design Standards.

ERIC Educational Resources Information Center

New York State Council on Parks and Outdoor Recreation, Albany.

Public outdoor recreational facilities and the needs of the physically handicapped are overviewed with guidelines to develop such facilities. Circulation and access provisions for wheel chairs and the semi-ambulatory are emphasized, including requirements for parking lots, pathways, resting areas and public bathrooms. Standard dimensions and…

Consumer Education. Information Supplements for Physically Disabled Students. Teacher's Guide.

ERIC Educational Resources Information Center

Tipsord, Barbara; And Others

This manual contains supplementary information for use by instructors who teach consumer education and resources management to physically handicapped students in regular classes. It is subdivided according to typical consumer education topics and handicapping conditions. Addressed in the individual sections of the manual are the folowing topics:…

[Physical handicapped, economic practices and matrimonial strategies in Senegal].

PubMed

Fassin, D

1991-01-01

Social relations around the handicapped are generally presented in terms of economic dependence and social inadaptation. This point of view leads to give greater importance, especially in Africa, to studying the way in which group and society help the physically or mentally ill. Actually, this approach does not give a complete account about the real situation of the handicapped in social relations of production and reproduction. From a series of in-depth interviews conducted in handicapped families of the suburbs of Dakar, two aspects are analyzed: the economic role of the handicapped, through the circulation of the product of his begging in his household or through the exploitation of his work as apprentice in a workshop; and his value on the marriage market, where invalid persons are given without dowry if they are women, and must pay a much more important amount if they are men. The social situation of the handicapped thus is not only a matter of assistance or charity, but as well of strategies that the handicapped and above all his circle implement in order to take advantage of the stigma or on the contrary try to erase it.

Nuclear targeting of the maize R protein requires two nuclear localization sequences

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shieh, M.W.; Raikhel, N.V.; Wessler, S.R.

1993-02-01

Previous genetic and structural evidence indicates that the maize R gene encodes a nuclear transcriptional activating factor. In-frame carboxyl- and amino-terminal fusions of the R gene to the reporter gene encoding [beta]-glucuronidase (GUS) were sufficient to direct GUS to the nucleus of the transiently transformed onion (Allium cepa) epidermal cells. Further analysis of chimeric constructs containing regions of the R gene fused to the GUS cDNA revealed three specific nuclear localization sequences (NLSs) that were capable of redirecting the GUS protein to the nucleus. Amino-terminal NLS-A (amino acids 100-109, GDRRAAPARP) contained several arginine residues; a similar localization signal is foundmore » in only a few viral proteins. The medial NLS-M (amino acids 419-428, MSERKRREKL) is a simian virus 40 large T antigen-type NLS, and the carboxyl-terminal NLS-C (amino acids 598-610, MISESLRKAIGKR) is a mating type [alpha]2 type. NLSs M and C are independently sufficient to direct the GUS protein to the nucleus when it is fused at the amino terminus of GUS, whereas NLS-A fused to GUS partitioned between the nucleus and cytoplasm. Similar partitioning was observed when localization signals NLS-A and NLS-C were independently fused to the carboxy-terminal portion of GUS. A sequential deletion of the localization signals indicated that the amino-terminal and carboxyl-terminal fusions of R and GUS were redirected to the nucleus only when both NLS-A and -M, or NLS-C and -M, were present. These results indicate that multiple localization signals are necessary for nuclear targeting of this protein. The conservation of the localization signals within the alleles of R and similar proteins from other organisms is also discussed. 45 refs., 6 figs.« less

Variations in Nuclear Localization Strategies Among Pol X Family Enzymes.

PubMed

Kirby, Thomas W; Pedersen, Lars C; Gabel, Scott A; Gassman, Natalie R; London, Robert E

2018-06-22

Despite the essential roles of pol X family enzymes in DNA repair, information about the structural basis of their nuclear import is limited. Recent studies revealed the unexpected presence of a functional NLS in DNA polymerase β, indicating the importance of active nuclear targeting, even for enzymes likely to leak into and out of the nucleus. The current studies further explore the active nuclear transport of these enzymes by identifying and structurally characterizing the functional NLS sequences in the three remaining human pol X enzymes: terminal deoxynucleotidyl transferase (TdT), DNA polymerase μ (pol μ), and DNA polymerase λ (pol λ). NLS identifications are based on Importin α (Impα) binding affinity determined by fluorescence polarization of fluorescein-labeled NLS peptides, X-ray crystallographic analysis of the Impα∆IBB•NLS complexes, and fluorescence-based subcellular localization studies. All three polymerases use NLS sequences located near their N-terminus; TdT and pol μ utilize monopartite NLS sequences, while pol λ utilizes a bipartite sequence, unique among the pol X family members. The pol μ NLS has relatively weak measured affinity for Impα, due in part to its proximity to the N-terminus that limits non-specific interactions of flanking residues preceding the NLS. However, this effect is partially mitigated by an N-terminal sequence unsupportive of Met1 removal by methionine aminopeptidase, leading to a 3-fold increase in affinity when the N-terminal methionine is present. Nuclear targeting is unique to each pol X family enzyme with variations dependent on the structure and unique functional role of each polymerase. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

The Davey-Stewartson Equation on the Half-Plane

NASA Astrophysics Data System (ADS)

Fokas, A. S.

2009-08-01

The Davey-Stewartson (DS) equation is a nonlinear integrable evolution equation in two spatial dimensions. It provides a multidimensional generalisation of the celebrated nonlinear Schrödinger (NLS) equation and it appears in several physical situations. The implementation of the Inverse Scattering Transform (IST) to the solution of the initial-value problem of the NLS was presented in 1972, whereas the analogous problem for the DS equation was solved in 1983. These results are based on the formulation and solution of certain classical problems in complex analysis, namely of a Riemann Hilbert problem (RH) and of either a d-bar or a non-local RH problem respectively. A method for solving the mathematically more complicated but physically more relevant case of boundary-value problems for evolution equations in one spatial dimension, like the NLS, was finally presented in 1997, after interjecting several novel ideas to the panoply of the IST methodology. Here, this method is further extended so that it can be applied to evolution equations in two spatial dimensions, like the DS equation. This novel extension involves several new steps, including the formulation of a d-bar problem for a sectionally non-analytic function, i.e. for a function which has different non-analytic representations in different domains of the complex plane. This, in addition to the computation of a d-bar derivative, also requires the computation of the relevant jumps across the different domains. This latter step has certain similarities (but is more complicated) with the corresponding step for those initial-value problems in two dimensions which can be solved via a non-local RH problem, like KPI.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aratani, Satoko; Oishi, Takayuki; Fujita, Hidetoshi

RNA helicase A (RHA), an ATPase/helicase, regulates the gene expression at various steps including transcriptional activation and RNA processing. RHA is known to shuttle between the nucleus and cytoplasm. We identified the nuclear localization signal (NLS) of RHA and analyzed the nuclear import mechanisms. The NLS of RHA (RHA-NLS) consisting of 19 amino acid residues is highly conserved through species and does not have the consensus classical NLS. In vitro nuclear import assays revealed that the nuclear import of RHA was Ran-dependent and mediated with the classical importin-{alpha}/{beta}-dependent pathway. The binding assay indicated that the basic residues in RHA-NLS weremore » used for interaction with importin-{alpha}. Furthermore, the nuclear import of RHA-NLS was supported by importin-{alpha}1 and preferentially importin-{alpha}3. Our results indicate that the nuclear import of RHA is mediated by the importin-{alpha}3/importin-{beta}-dependent pathway and suggest that the specificity for importin may regulate the functions of cargo proteins.« less

Selecting Library Materials for Exceptional Children.

ERIC Educational Resources Information Center

Harris, Karen H.

1979-01-01

Presents principles for selecting library materials appropriate for physically handicapped, mentally retarded, and emotionally disturbed children, and for promoting understanding of the handicapped by nonimpaired children. (CMV)

DIRECTORY OF CAMPS FOR THE HANDICAPPED.

ERIC Educational Resources Information Center

National Easter Seal Society for Crippled Children and Adults, Chicago, IL.

ONE HUNDRED AND SEVENTY-SEVEN RESIDENT CAMPS IN THE UNITED STATES AND CANADA AND 77 DAY CAMPS IN THE UNITED STATES WHICH SERVE CHILDREN OR ADULTS WITH PHYSICAL, MENTAL, SOCIAL, AND EMOTIONAL HANDICAPS ARE LISTED ALPHABETICALLY BY STATE. FOR EACH CAMP, INFORMATION ON TYPES OF THE HANDICAPPED WHO ARE ACCEPTED, SPECIFIC EXCLUSIONS, AGE RANGE, NUMBER…

BCRMD Program for Multiply Handicapped Pupils: Evaluation Report. School Year 1975-1976.

ERIC Educational Resources Information Center

Echternacht, Gary

Presented is an evaluation of a program which provided supplementary pre-reading and mathematics instruction to multiply handicapped children (physically and mentally handicapped) in six elementary and two intermediate schools. It is explained that students were individually instructed on objectives which they failed on the McGraw Hill Test of…

Alabama School for the Blind Fitness and Nutrition Project.

ERIC Educational Resources Information Center

Patterson, Douglas

This project compared the relative physical fitness levels of 30 visually handicapped/blind students (aged 7-18) at a residential school with that of non-handicapped peers, to develop a school-based fitness and nutrition intervention program and determine impact of the intervention. The compounding handicapping nature of fitness deficits and…

Oklahoma Curriculum Guide for Teaching Driver Education to the Handicapped.

ERIC Educational Resources Information Center

1973

This booklet is intended to aid driver education instructors and supervisors to develop a program of driver education for physically or mentally handicapped persons. The guide is organized in five sections. The first section introduces the concept of driver education for the handicapped, and relates its history, philosophy, objectives for a…

Access Guide to South Carolina State Parks for People with Special Needs.

ERIC Educational Resources Information Center

South Carolina State Dept. of Parks, Recreation, and Tourism, Columbia. Div. of Engineering and Planning.

The guide was developed to assist physically handicapped persons in using South Carolina State Parks. It describes some of the accessibility problems identified in a 1986 Inventory of Handicapped Accessibility in South Carolina State Parks and Welcome Centers. It is noted that building construction since 1967 has met handicapped design criteria…

The Vulnerable Child. Caring for Children, Number Five.

ERIC Educational Resources Information Center

Murphy, Lois B.; Leeper, Ethel M.

The booklet addresses the problems of vulnerable children with suggestions child care center workers may use both for recognizing signs of handicaps in children and helping children overcome existing handicaps. The vulnerable child is defined as a child who may be overwhelmed by physical and/or emotional handicaps and thus is at risk of later…

Vocational Home Economics for the Mainstreamed Physically Handicapped Student: The Least Restrictive Environment.

ERIC Educational Resources Information Center

Olson, Susan J.

School building and classroom accessibility are the first factors considered in a discussion of making provisions for mainstreamed physically handicapped students in vocational home economics programs. Legal mandates are cited, and ideas for adapting food labs and sewing labs for wheelchair students and students with visual impairments are noted.…

Safari Adventures: A Bibliography for Young Readers Preschool through Junior High.

ERIC Educational Resources Information Center

Sumner, Mary Ann, Comp.

This annotated bibliography contains books for blind or physically handicapped youngsters from preschool through junior high. The books are available in braille, on audiocassette, or recorded discs. The approximately 400 titles listed were produced in Florida or obtained from the National Library Service for the Blind and Physically Handicapped.…

Occupational Opportunities for the Physically Handicapped. Part B. Manual.

ERIC Educational Resources Information Center

Uthe, Elaine F.

This manual presents the master lists of 206 job titles of 167 different Dictionary of Occupational Titles (DOT) code numbers which were held by physically handicapped graduates/completers of vocational programs as determined by a business and industry survey and graduate followup. (The project itself is reported in CE 026 163; survey and followup…

Barrier Free Design Handbook: Accommodations for the Physically Handicapped.

ERIC Educational Resources Information Center

Veterans Administration, Washington, DC. Office of Construction.

The handbook sets forth design requirements for construction and renovation of buildings owned, leased, or funded by the Veterans Administration (VA), to permit physically handicapped persons ready access. The book is intended mainly for use by the VA staff and contracted architect/engineer firms involved in developing criteria and designing,…

Summer Education Program for Neurologically and Physically Handicapped Children. Summer 1975. Evaluation Report.

ERIC Educational Resources Information Center

Ellis, Ronald S.

Evaluated was the Summer Education Program for Neurologically and Physically Handicapped Children, designed to improve the performance of 145 children (6-16 years old) in the following areas--gross motor skills, swimming, fine motor skills, socialization with nonhandicapped peers, and independent daily living skills. The program included the…

Hearing-aid use and long-term health outcomes: Hearing handicap, mental health, social engagement, cognitive function, physical health, and mortality.

PubMed

Dawes, Piers; Cruickshanks, Karen J; Fischer, Mary E; Klein, Barbara E K; Klein, Ronald; Nondahl, David M

2015-01-01

To clarify the impact of hearing aids on mental health, social engagement, cognitive function, and physical health outcomes in older adults with hearing impairment. We assessed hearing handicap (hearing handicap inventory for the elderly; HHIE-S), cognition (mini mental state exam, trail making, auditory verbal learning, digit-symbol substitution, verbal fluency, incidence of cognitive impairment), physical health (SF-12 physical component, basic and instrumental activities of daily living, mortality), social engagement (hours per week spent in solitary activities), and mental health (SF-12 mental component) at baseline, five years prior to baseline, and five and 11 years after baseline. Community-dwelling older adults with hearing impairment (N = 666) from the epidemiology of hearing loss study cohort. There were no significant differences between hearing-aid users and non-users in cognitive, social engagement, or mental health outcomes at any time point. Aided HHIE-S was significantly better than unaided HHIE-S. At 11 years hearing-aid users had significantly better SF-12 physical health scores (46.2 versus 41.2; p = 0.03). There was no difference in incidence of cognitive impairment or mortality. There was no evidence that hearing aids promote cognitive function, mental health, or social engagement. Hearing aids may reduce hearing handicap and promote better physical health.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klucevsek, K.; Daley, J.; Darshan, M.S.

We have investigated the nuclear import strategies of high-risk HPV18 L2 minor capsid protein. HPV18 L2 interacts with Kap {alpha}{sub 2} adapter, and Kap {beta}{sub 2} and Kap {beta}{sub 3} nuclear import receptors. Moreover, binding of RanGTP to either Kap {beta}{sub 2} or Kap {beta}{sub 3} inhibits their interaction with L2, suggesting that these Kap {beta}/L2 complexes are import competent. Mapping studies show that HPV18 L2 contains two NLSs: in the N-terminus (nNLS) and in the C-terminus (cNLS), both of which can independently mediate nuclear import. Both nNLS and cNLS form a complex with Kap {alpha}{sub 2}{beta}{sub 1} heterodimer andmore » mediate nuclear import via a classical pathway. The nNLS is also essential for the interaction of HPV18 L2 with Kap {beta}{sub 2} and Kap {beta}{sub 3}. Interestingly, both nNLS and cNLS interact with the viral DNA and this DNA binding occurs without nucleotide sequence specificity. Together, the data suggest that HPV18 L2 can interact via its NLSs with several Kaps and the viral DNA and may enter the nucleus via multiple import pathways mediated by Kap {alpha}{sub 2}{beta}{sub 1} heterodimers, Kap {beta}{sub 2} and Kap {beta}{sub 3}.« less

Nuclear localization of the DNA repair scaffold XRCC1: Uncovering the functional role of a bipartite NLS

DOE PAGES

Kirby, Thomas W.; Gassman, Natalie R.; Smith, Cassandra E.; ...

2015-08-25

We have characterized the nuclear localization signal (NLS) of XRCC1 structurally using X-ray crystallography and functionally using fluorescence imaging. Crystallography and binding studies confirm the bipartite nature of the XRCC1 NLS interaction with Importin α (Impα) in which the major and minor binding motifs are separated by >20 residues, and resolve previous inconsistent determinations. Binding studies of peptides corresponding to the bipartite NLS, as well as its major and minor binding motifs, to both wild-type and mutated forms of Impα reveal pronounced cooperative binding behavior that is generated by the proximity effect of the tethered major and minor motifs ofmore » the NLS. The cooperativity stems from the increased local concentration of the second motif near its cognate binding site that is a consequence of the stepwise binding behavior of the bipartite NLS. We predict that the stepwise dissociation of the NLS from Impα facilitates unloading by providing a partially complexed intermediate that is available for competitive binding by Nup50 or the Importin β binding domain. This behavior gives a basis for meeting the intrinsically conflicting high affinity and high flux requirements of an efficient nuclear transport system.« less

7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis

PubMed Central

van Gelderen, Peter; Merkle, Hellmuth; Chen, Christina; Lassmann, Hans; Duyn, Jeff H.; Bagnato, Francesca

2014-01-01

Background Neocortical lesions (NLs) are an important pathological component of multiple sclerosis (MS), but their visualization by magnetic resonance imaging (MRI) remains challenging. Objectives We aimed at assessing the sensitivity of multi echo gradient echo (ME-GRE) T2 *-weighted MRI at 7.0 Tesla in depicting NLs compared to myelin and iron staining. Methods Samples from two MS patients were imaged post mortem using a whole body 7T MRI scanner with a 24-channel receive-only array. Isotropic 200 micron resolution images with varying T2 * weighting were reconstructed from the ME-GRE data and converted into R2 * maps. Immunohistochemical staining for myelin (proteolipid protein, PLP) and diaminobenzidine-enhanced Turnbull blue staining for iron were performed. Results Prospective and retrospective sensitivities of MRI for the detection of NLs were 48% and 67% respectively. We observed MRI maps detecting only a small portion of 20 subpial NLs extending over large cortical areas on PLP stainings. No MRI signal changes suggestive of iron accumulation in NLs were observed. Conversely, R2 * maps indicated iron loss in NLs, which was confirmed by histological quantification. Conclusions High-resolution post mortem imaging using R2 * and magnitude maps permits detection of focal NLs. However, disclosing extensive subpial demyelination with MRI remains challenging. PMID:25303286

Guidelines to birth control counselling of the physically handicapped.

PubMed Central

Szasz, G.; Miller, S.; Anderson, L.

1979-01-01

Birth control counselling of well motivated physically handicapped men and women is discussed in a series of suggested steps: (1) preappointment arrangements are made to ensure physical access to the office, and the partner or a helper may be invited to the visit; (2) the patient is encouraged to name the desired birth control method; (3) the patient's fertility is established from the history and the results of physical examination and laboratory tests; (4) methods of birth control are matched to the patient's physical and mental capabilities; (5) potential side effects of the proposed methods are considered in the light of the handicap; (6) a method is finally recommended that may be a trade-off, with ease of use balanced against efficacy, and living with side effects balanced against the risks of pregnancy; and (7) rechecks are necessary to ensure that the patient is using the method with skill and satisfaction. PMID:156579

7 CFR 15b.31 - Treatment of students.

Code of Federal Regulations, 2013 CFR

2013-01-01

..., research, occupational training, housing, health, insurance, counseling, financial aid, physical education... basis of handicap, exclude any qualified handicapped student from any course, course of study, or other...

7 CFR 15b.31 - Treatment of students.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., research, occupational training, housing, health, insurance, counseling, financial aid, physical education... basis of handicap, exclude any qualified handicapped student from any course, course of study, or other...

7 CFR 15b.31 - Treatment of students.

Code of Federal Regulations, 2012 CFR

2012-01-01

..., research, occupational training, housing, health, insurance, counseling, financial aid, physical education... basis of handicap, exclude any qualified handicapped student from any course, course of study, or other...

7 CFR 15b.31 - Treatment of students.

Code of Federal Regulations, 2014 CFR

2014-01-01

..., research, occupational training, housing, health, insurance, counseling, financial aid, physical education... basis of handicap, exclude any qualified handicapped student from any course, course of study, or other...

Art Therapy: A Creative Approach to the Care of Mentally Handicapped People.

ERIC Educational Resources Information Center

Males, Jeanne; Males, Bruce

1979-01-01

The article describes the art therapy program for the mentally handicapped at St. Lawrence's Hospital in England. The program, which also serves those retarded patients who are physically handicapped, functions as a diagnosic and assessment aid, direct therapeutic aid, part of an integrated treatment program, means of employment for some patients,…

Association for Education of the Visually Handicapped Biennial Conference (Forty-Ninth, Toronto, Canada, June 1968).

ERIC Educational Resources Information Center

Association for Education of the Visually Handicapped, Philadelphia, PA.

Essays on the visually handicapped are concerned with congenital rubella, an evaluation of multiply handicapped children, the use and abuse of the IQ, visual perception dysfunction, spatial perceptions in the partially sighted, programs in daily living skills, sex education needs, and physical activity as an enhancement of functioning. Other…

KI-Aikido for Handicapped Students at Leeward Community College: Theory and Practice.

ERIC Educational Resources Information Center

MacGugan, Kirk

In an effort to provide physical education instruction for handicapped students, Leeward Community College implemented, on a pilot basis, a non-credit course in KI-Aikido, an oriental martial art which combines theory and exercise toward the goal of controlling the body through the power of the mind. The course, offered to both handicapped and…

Late-assembly of human ribosomal protein S20 in the cytoplasm is essential for the functioning of the small subunit ribosome

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tai, Lin-Ru; Chou, Chang-Wei; Wu, Jing-Ying

2013-11-15

Using immuno-fluorescent probing and Western blotting analysis, we reveal the exclusive cytoplasm nature of the small subunit ribosomal protein S20. To illustrate the importance of the cellular compartmentation of S20 to the function of small subunit 40S, we created a nuclear resident S20{sub NLS} mutant gene and examined polysome profile of cells that had been transfected with the S20{sub NLS} gene. As a result, we observed the formation of recombinant 40S carried S20{sub NLS} but this recombinant 40S was never found in the polysome, suggesting such a recombinant 40S was translation incompetent. Moreover, by the tactic of the energy depletionmore » and restoration, we were able to restrain the nuclear-resided S20{sub NLS} in the cytoplasm. Yet, along a progressive energy restoration, we observed the presence of recombinant 40S subunits carrying the S20{sub NLS} in the polysome. This proves that S20 needs to be cytoplasmic in order to make a functional 40S subunit. Furthermore, it also implies that the assembly order of ribosomal protein in eukaryote is orderly regulated. - Highlights: • The step of S20 assembled on 40S is happened in the cytoplasm. • A small subunit assembled with a nuclear S20{sub NLS} is translational incompetence. • Using energy depletion and recovery to manipulate the cellular compartment of S20{sub NLS}. • Cytoplasm-retained S20{sub NLS} is crucial for creating a functional small subunit.« less

Nuclear Import and Export of the Thyroid Hormone Receptor.

PubMed

Zhang, Jibo; Roggero, Vincent R; Allison, Lizabeth A

2018-01-01

The thyroid hormone receptors, TRα1 and TRβ1, are members of the nuclear receptor superfamily that forms one of the most abundant classes of transcription factors in multicellular organisms. Although primarily localized to the nucleus, TRα1 and TRβ1 shuttle rapidly between the nucleus and cytoplasm. The fine balance between nuclear import and export of TRs has emerged as a critical control point for modulating thyroid hormone-responsive gene expression. Mutagenesis studies have defined two nuclear localization signal (NLS) motifs that direct nuclear import of TRα1: NLS-1 in the hinge domain and NLS-2 in the N-terminal A/B domain. Three nuclear export signal (NES) motifs reside in the ligand-binding domain. A combined approach of shRNA-mediated knockdown and coimmunoprecipitation assays revealed that nuclear entry of TRα1 is facilitated by importin 7, likely through interactions with NLS-2, and importin β1 and the adapter importin α1 interacting with both NLS-1 and NLS-2. Interestingly, TRβ1 lacks NLS-2 and nuclear import depends solely on the importin α1/β1 heterodimer. Heterokaryon and fluorescence recovery after photobleaching shuttling assays identified multiple exportins that play a role in nuclear export of TRα1, including CRM1 (exportin 1), and exportins 4, 5, and 7. Even single amino acid changes in TRs dramatically alter their intracellular distribution patterns. We conclude that mutations within NLS and NES motifs affect nuclear shuttling activity, and propose that TR mislocalization contributes to the development of some types of cancer and Resistance to Thyroid Hormone syndrome. © 2018 Elsevier Inc. All rights reserved.

Occupational Counseling for Physically Handicapped Students: A Demonstration and Training Project.

ERIC Educational Resources Information Center

Goldman, Leo; Flugman, Bert

Presented is a report of a project to provide occupational counseling to over 60 physically handicapped high school students. Noted are project goals involving direct and indirect provision of services to the students, the training of counselors through a 1-year internship, and the development of a model for a counseling specialty within the…

Guide to Information Systems in Physical Education and Recreation for Impaired, Disabled, and Handicapped Persons.

ERIC Educational Resources Information Center

Summerfield, Liane, Ed.

The information systems/centers listed in this guide are organized into the following five sections according to their area of concentration: (1) Education/Special Education; (2) the Sciences; (3) Physical Education, Recreation, and Related Areas; (4) Products and Services for Handicapped Consumers; and (5) Other. The systems and centers have also…

36 CFR 701.6 - Loans of library materials for blind and other physically handicapped persons.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Loans of library materials... Property LIBRARY OF CONGRESS PROCEDURES AND SERVICES § 701.6 Loans of library materials for blind and other physically handicapped persons. (a) Program. In connection with the Library's program of service under the...

36 CFR 701.6 - Loans of library materials for blind and other physically handicapped persons.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Loans of library materials... Property LIBRARY OF CONGRESS PROCEDURES AND SERVICES § 701.6 Loans of library materials for blind and other physically handicapped persons. (a) Program. In connection with the Library's program of service under the...

Effects of a Training Program on the Job Readiness of Physically Handicapped Sheltered Workshop Employees.

ERIC Educational Resources Information Center

Tesolowski, Dennis G.; Halpin, Gerald

Twenty-two physically handicapped sheltered workshop employees were matched on the severity of their disabilities and randomly assigned to either an experimental or control group. Those in the experimental group participated in a one-hour-per-day, fifteen-day job readiness training program in addition to their regular sheltered workshop jobs while…

33 CFR 151.35 - Certificates needed to carry Category D NLS and Category D Oil-like NLS.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Protocol on Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships Noxious Liquid Substance Pollution § 151.35 Certificates needed to carry Category D NLS and Category D Oil-like... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION VESSELS CARRYING OIL, NOXIOUS LIQUID...

[Psychosocial adjustment of the handicapped child--possibilities and problems of rehabilitation].

PubMed

Eulitz, R

1989-01-01

A successful childhood is to be striven after particularly for a handicapped child. Habilitation and rehabilitation, only carefully adapted to one another referring to medical specialties in colaboration with the family, may rise to the demand of the individuality of every one handicapped child. On prior conditions children with chiefly physical handicaps may be integrated into the polytechnical secondary school providing general education in the home town. The total-body examination of these children remains to be the basis for all paediatric actions.

National Launch System comparative economic analysis

NASA Technical Reports Server (NTRS)

Prince, A.

1992-01-01

Results are presented from an analysis of economic benefits (or losses), in the form of the life cycle cost savings, resulting from the development of the National Launch System (NLS) family of launch vehicles. The analysis was carried out by comparing various NLS-based architectures with the current Shuttle/Titan IV fleet. The basic methodology behind this NLS analysis was to develop a set of annual payload requirements for the Space Station Freedom and LEO, to design launch vehicle architectures around these requirements, and to perform life-cycle cost analyses on all of the architectures. A SEI requirement was included. Launch failure costs were estimated and combined with the relative reliability assumptions to measure the effects of losses. Based on the analysis, a Shuttle/NLS architecture evolving into a pressurized-logistics-carrier/NLS architecture appears to offer the best long-term cost benefit.

Role of SIRT1-mediated mitochondrial and Akt pathways in glioblastoma cell death induced by Cotinus coggygria flavonoid nanoliposomes

PubMed Central

Wang, Gang; Wang, Jun Jie; To, Tony SS; Zhao, Hua Fu; Wang, Jing

2015-01-01

Flavonoids, the major polyphenol components in Cotinus coggygria (CC), have been found to show an anticancer effect in our previous study; however, the exact mechanisms of inducing human glioblastoma (GBM) cell death remain to be resolved. In this study, a novel polyvinylpyrrolidone K-30/sodium dodecyl sulfate and polyethyleneglycol-coated liposome loaded with CC flavonoids (CCFs) was developed to enhance solubility and the antibrain tumor effect, and the molecular mechanism regarding how CCF nanoliposomes (CCF-NLs) induce apoptotic cell death in vitro was investigated. DBTRG-05MG GBM cell lines treated with CCF-NLs showed potential antiproliferative effects. Regarding the underlying mechanisms of inducing apoptosis in DBTRG-05MG GBM cells, CCF-NLs were shown to downregulate the expression of antiapoptotic B-cell lymphoma/leukemia 2 (Bcl-2), an apoptosis-related protein family member, but the expression of proapoptotic Bcl-2-associated X protein was enhanced compared with that in controls. CCF-NLs also inhibited the activity of caspase-3 and -9, which is the initiator caspase of the extrinsic and intrinsic apoptotic pathways. Blockade of caspase activation consistently induced apoptosis and inhibited growth in CCF-NL-treated DBTRG-05MG cells. This study further investigated the role of the Akt pathway in the apoptotic cell death by CCF-NLs, showing that CCF-NLs deactivated Akt. Specifically, CCF-NLs downregulated the expression of p-Akt and SIRT1 as well as the level of phosphorylated p53. Together, these results indicated SIRT1/p53-mediated cell death was induced by CCF-NLs, but not by extracellular signal-regulated kinase, in DBTRG-05MG cells. Overall, this study suggested caspase-dependent activation of both the intrinsic and extrinsic signaling pathways, probably through blockade of the SIRT1/p53-mediated mitochondrial and Akt pathways to exert the proapoptotic effect of CCF-NLs in DBTRG-05MG GBM cells. PMID:26345416

Semiclassical limit of the focusing NLS: Whitham equations and the Riemann-Hilbert Problem approach

NASA Astrophysics Data System (ADS)

Tovbis, Alexander; El, Gennady A.

2016-10-01

The main goal of this paper is to put together: a) the Whitham theory applicable to slowly modulated N-phase nonlinear wave solutions to the focusing nonlinear Schrödinger (fNLS) equation, and b) the Riemann-Hilbert Problem approach to particular solutions of the fNLS in the semiclassical (small dispersion) limit that develop slowly modulated N-phase nonlinear wave in the process of evolution. Both approaches have their own merits and limitations. Understanding of the interrelations between them could prove beneficial for a broad range of problems involving the semiclassical fNLS.

Integrability and structural stability of solutions to the Ginzburg-Landau equation

NASA Technical Reports Server (NTRS)

Keefe, Laurence R.

1986-01-01

The integrability of the Ginzburg-Landau equation is studied to investigate if the existence of chaotic solutions found numerically could have been predicted a priori. The equation is shown not to possess the Painleveproperty, except for a special case of the coefficients that corresponds to the integrable, nonlinear Schroedinger (NLS) equation. Regarding the Ginzburg-Landau equation as a dissipative perturbation of the NLS, numerical experiments show all but one of a family of two-tori solutions, possessed by the NLS under particular conditions, to disappear under real perturbations to the NLS coefficients of O(10 to the -6th).

Self-handicapping in school physical education: The influence of the motivational climate.

PubMed

Standage, Martyn; Treasure, Darren C; Hooper, Katherine; Kuczka, Kendy

2007-03-01

Self-handicapping is an attribution-related process whereby individuals create performance impediments/excuses to protect self-worth in socially evaluative environments. Thus, the prevailing motivational climate would appear to be an important factor when attempting to understand the situational self-handicapping process within school physical education. Drawing from achievement goal theory, the study examined the effect of experimentally induced conditions (viz. task vs. ego) on situational self-handicapping. Seventy British secondary school students (36 females and 34 males; M age = 11.98; SD=0.31). Participants were randomly assigned to partake in a running endurance task in either an ego-involving (20 male students and 16 female students) or a task-involving (14 male students and 20 female students) condition. Prior to completing the experimental task, participants were given the opportunity to claim situational self-handicaps. Data for goal orientations, subjective climate perceptions, perceived ability and perceived task importance were also obtained. After determining the effectiveness of the experimental manipulation, results revealed participants in the ego-involving condition to report significantly more situational self-handicapping claims. Further, and after controlling for individual difference variables, the results of moderated hierarchical regression analysis revealed subjective perceptions of an ego-involving climate to be the main positive predictor of situational self-handicapping. Although a weaker contributor to the percentage of variance explained, task orientation emerged as a negative predictor of situational self-handicapping. The findings suggest that PE teachers would be prudent to minimize ego-involving situations should they wish to reduce situational self-handicapping.

Association of Neglect-Like Symptoms with Anxiety, Somatization, and Depersonalization in Complex Regional Pain Syndrome.

PubMed

Michal, Matthias; Adler, Julia; Reiner, Iris; Wermke, Andreas; Ackermann, Tatiana; Schlereth, Tanja; Birklein, Frank

2017-04-01

Many patients with complex regional pain syndrome (CRPS) report some foreignness of the affected limb, which is referred to as "neglect-like symptoms" (NLS). Despite similarities of the NLS reports to symptoms of body image disturbances in mental disorders, no study has been conducted to examine such associations. We investigated 50 patients with CRPS and 45 pain control patients (N = 27, chronic limb pain; N = 18, migraine headache). NLS, anxiety, depression, depersonalization, and somatization were assessed using validated questionnaires. Seventy-two percent of the CRPS patients reported at least one NLS vs 29.6% and 33.3% in the two patient control groups. In limb pain controls, NLS correlated with pain intensity. In CRPS patients, NLS correlated with anxiety (rho = 0.658, P  < 0.001), somatization (rho = 0.616, P  < 0.001), depersonalization (rho = 0.634, P  < 0.001), and pain catastrophizing (rho = 0.456, P  < 0.01), but not with intensity of pain, duration of pain, or pain disability. In CRPS patients, NLS could be a result of somatization, depression, anxiety, and depersonalization, but probably not of pain. Whether these associations are causative must be clarified in longitudinal psychological studies. © 2016 American Academy of Pain Medicine. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

Relations between female students' personality traits and reported handicaps to rhythmic gymnastics performance.

PubMed

Ferrand, Claude; Champely, Stephane; Brunel, Philippe C

2005-04-01

The present study evaluated the relative contributions of Self-esteem, Trait anxiety, and Public Self-consciousness to self-handicapping on a sex-typed task, within a specific academic sport context. Prior to the competitive examination used to recruit French Physical Education Teachers, female sport students (N = 74) were asked to list and rate on a 7-point scale handicaps which could be disruptive to their Rhythmic Gymnastics performance. Self-esteem did not account for significant variance in any category of handicaps. Trait Anxiety was negatively related to handicaps related to Rhythmic Gymnastics and to Social and Work Commitments. Public Self-consciousness was significantly related to endorsement of Friends and Family Commitments handicaps. These results were discussed in relation to the literature.

Hearing handicap, rather than measured hearing impairment, predicts poorer quality of life over 10 years in older adults.

PubMed

Gopinath, Bamini; Schneider, Julie; Hickson, Louise; McMahon, Catherine M; Burlutsky, George; Leeder, Stephen R; Mitchell, Paul

2012-06-01

We aimed to determine the prospective association between measured hearing impairment, self-reported hearing handicap and hearing aid use with quality of life. 829 Blue Mountains Hearing Study participants (≥ 55 years) were examined between 1997-1999 and 2007-2009. The shortened version of the hearing handicap inventory was administered. Hearing levels were measured using pure-tone audiometry. Quality of life was assessed using the 36-Item Short-Form Survey (SF-36); higher scores reflect better quality of life. Hearing impairment at baseline compared with no impairment was associated with lower mean SF-36 mental composite score 10 years later (multivariable-adjusted p=0.03). Physical composite score and mean scores for seven of the eight SF-36 domains after 10-year follow-up were significantly lower among participants who self-reported hearing handicap at baseline. Differences in the adjusted means between participants with and without hearing handicap ranged from 2.7 (physical composite score) to 10.4 units ('role limitations due to physical problems' domain). Individuals who developed incident hearing impairment compared to those who did not, had adjusted mean scores 9.5- and 7.7-units lower in the 'role limitation due to physical problems', and 'bodily pain' domains, respectively, at the 10-year follow-up. Hearing aid users versus non-users at baseline showed a 1.82-point (p=0.03) and 3.32-point (p=0.01) increase in SF-36 mental composite score and mental health domain over the 10-year follow-up, respectively. Older adults with self-perceived hearing handicap constitute a potential risk group for overall deterioration in quality of life, while hearing aid use could help improve the well-being of hearing impaired adults. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

46 CFR 151.12-10 - Operation of oceangoing non-self-propelled ships Carrying Category D NLS.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 5 2011-10-01 2011-10-01 false Operation of oceangoing non-self-propelled ships... and Operating Requirements for Control of Pollution From Category D NLS Cargoes § 151.12-10 Operation of oceangoing non-self-propelled ships Carrying Category D NLS. (a) An oceangoing non-self-propelled...

Reading the Future: NLS Partnerships Help Canadians Meet the Challenges of a Changing World.

ERIC Educational Resources Information Center

National Literacy Secretariat, Ottawa (Ontario).

Canada's National Literacy Secretariat (NLS) develops innovative partnerships to involve voluntary and national organizations, governments, business, and labor in a national effort to make Canada a more literate society. The two main objectives of the NLS are to promote a variety of opportunities for people to improve their reading and writing…

Getting Through College With a Disability; A Summary of Services Available on 500 Campuses for Students With Handicapping Conditions.

ERIC Educational Resources Information Center

President's Committee on Employment of the Handicapped, Washington, DC.

This guide to college selection for the physically handicapped student summarizes information on the services and policies of 500 two- and four-year colleges. As to each college, it indicates if there are student organizations, offices, financial aid services, or career development services specifically designed for handicapped students, and if…

The lack of antiepileptic drugs and worsening of seizures among physically handicapped patients with epilepsy during the Great East Japan Earthquake.

PubMed

Kobayashi, Satoru; Endo, Wakaba; Inui, Takehiko; Wakusawa, Keisuke; Tanaka, Soichiro; Onuma, Akira; Haginoya, Kazuhiro

2016-08-01

Takuto Rehabilitation Center for Children is located in Sendai, the capital of the Miyagi prefecture, and faces the Pacific Ocean. The tsunami caused by the Great East Japan Earthquake resulted in tremendous damage to this region. Many physically handicapped patients with epilepsy who are treated at our hospital could not obtain medicine. We surveyed patients with epilepsy, using a questionnaire to identify the problems during the acute phase of the Great East Japan Earthquake. After the earthquake, we mailed questionnaires to physically handicapped patients with epilepsy who are treated and prescribed medications at our hospital, or to their parents. A total of 161 respondents completed the questionnaire. Overall, 68.4% of patients had seven days or less of stockpiled medication when the earthquake initially struck, and 28.6% of patients had no medication or almost no medication during the acute phase after the earthquake. Six patients were forced to stop taking their medication and nine patients experienced a worsening of seizures. Most (93.6%) patients stated they require a stockpile of medication for more than seven days: 20months after the earthquake, 76.9% patients a supply of drugs for more than seven days. We suggest that physically handicapped patients with epilepsy are recommended to prepare for natural disasters by stockpiling additional medication. Even if the stock of antiepileptic drugs is sufficient, stress could cause worsening of seizures. Specialized support is required after a disaster among physically handicapped patients with epilepsy. Copyright © 2016 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved.

Analysis of a developmentally regulated nuclear localization signal in Xenopus

PubMed Central

1992-01-01

The 289 residue nuclear oncoprotein encoded by the adenovirus 5 Ela gene contains two peptide sequences that behave as nuclear localization signals (NLS). One signal, located at the carboxy terminus, is like many other known NLSs in that it consists of a short stretch of basic residues (KRPRP) and is constitutively active in cells. The second signal resides within an internal 45 residue region of E1a that contains few basic residues or sequences that resemble other known NLSs. Moreover, this internal signal functions in injected Xenopus oocytes, but not in transfected Xenopus A6 cells, suggesting that it could be regulated developmentally (Slavicek et al. 1989. J. Virol. 63:4047). In this study, we show that the activity of this signal is sensitive to ATP depletion in vivo, efficiently directs the import of a 50 kD fusion protein and can compete with the E1a carboxy-terminal NLS for nuclear import. In addition, we have delineated the precise amino acid residues that comprise the second E1a NLS, and have assessed its utilization during Xenopus embryogenesis. Using amino acid deletion and substitution analyses, we show that the signal consists of the sequence FV(X)7-20MXSLXYM(X)4MF. By expressing in Xenopus embryos a truncated E1a protein that contains only the second NLS and by monitoring its cytoplasmic/nuclear distribution during development with indirect immunofluorescence, we find that the second NLS is utilized up to the early neurula stage. In addition, there appears to be a hierarchy among the embryonic germ layers as to when the second NLS becomes nonfunctional. For this reason, we refer to this NLS as the developmentally regulated nuclear localization signal (drNLS). The implications of these findings for early development are discussed. PMID:1387407

Backscattering and Nonparaxiality Arrest Collapse of Damped Nonlinear Waves

NASA Technical Reports Server (NTRS)

Fibich, G.; Ilan, B.; Tsynkov, S.

2002-01-01

The critical nonlinear Schrodinger equation (NLS) models the propagation of intense laser light in Kerr media. This equation is derived from the more comprehensive nonlinear Helmholtz equation (NLH) by employing the paraxial approximation and neglecting the backscattered waves. It is known that if the input power of the laser beam (i.e., L(sub 2) norm of the initial solution) is sufficiently high, then the NLS model predicts that the beam will self-focus to a point (i.e.. collapse) at a finite propagation distance. Mathematically, this behavior corresponds to the formation of a singularity in the solution of the NLS. A key question which has been open for many years is whether the solution to the NLH, i.e., the 'parent' equation, may nonetheless exist and remain regular everywhere, in particular for those initial conditions (input powers) that lead to blowup in the NLS. In the current study, we address this question by introducing linear damping into both models and subsequently comparing the numerical solutions of the damped NLH (boundary-value problem) with the corresponding solutions of the damped NLS (initial-value problem). Linear damping is introduced in much the same way as done when analyzing the classical constant-coefficient Helmholtz equation using the limiting absorption principle. Numerically, we have found that it provides a very efficient tool for controlling the solutions of both the NLH and NHS. In particular, we have been able to identify initial conditions for which the NLS solution does become singular. whereas the NLH solution still remains regular everywhere. We believe that our finding of a larger domain of existence for the NLH than that for the NLS is accounted for by precisely those mechanisms, that have been neglected when deriving the NLS from the NLH, i.e., nonparaxiality and backscattering.

The compensatory G88R change is essential in restoring the normal functions of influenza A/WSN/33 virus matrix protein 1 with a disrupted nuclear localization signal.

PubMed

Xie, Hang; Lin, Zhengshi; Mosier, Philip D; Desai, Umesh R; Gao, Yamei

2013-01-01

G88R emerged as a compensatory mutation in matrix protein 1 (M1) of influenza virus A/WSN/33 when its nuclear localization signal (NLS) was disrupted by R101S and R105S substitutions. The resultant M1 triple mutant M(NLS-88R) regained replication efficiency in vitro while remaining attenuated in vivo with the potential of being a live vaccine candidate. To understand why G88R was favored by the virus as a compensatory change for the NLS loss and resultant replication deficiency, three more M1 triple mutants with an alternative G88K, G88V, or G88E change in addition to R101S and R105S substitutions in the NLS were generated. Unlike the other M1 triple mutants, M(NLS-88R) replicated more efficiently in vitro and in vivo. The G88R compensatory mutation not only restored normal functions of M1 in the presence of a disrupted NLS but also resulted in a strong association of M1 with viral ribonucleoprotein. Under a transmission electron microscope, only the M1 layer of the M(NLS-88R) virion exhibited discontinuous fingerprint-like patterns with average thicknesses close to that of wild-type A/WSN/33. Computational modeling suggested that the compensatory G88R change could reestablish the integrity of the M1 layer through new salt bridges between adjacent M1 subunits when the original interactions were interrupted by simultaneous R101S and R105S replacements in the NLS. Our results suggested that restoring the normal functions of M1 was crucial for efficient virus replication.

Spectral stability of shifted states on star graphs

NASA Astrophysics Data System (ADS)

Kairzhan, Adilbek; Pelinovsky, Dmitry E.

2018-03-01

We consider the nonlinear Schrödinger (NLS) equation with the subcritical power nonlinearity on a star graph consisting of N edges and a single vertex under generalized Kirchhoff boundary conditions. The stationary NLS equation may admit a family of solitary waves parameterized by a translational parameter, which we call the shifted states. The two main examples include (i) the star graph with even N under the classical Kirchhoff boundary conditions and (ii) the star graph with one incoming edge and N  -  1 outgoing edges under a single constraint on coefficients of the generalized Kirchhoff boundary conditions. We obtain the general counting results on the Morse index of the shifted states and apply them to the two examples. In the case of (i), we prove that the shifted states with even N ≥slant 4 are saddle points of the action functional which are spectrally unstable under the NLS flow. In the case of (ii), we prove that the shifted states with the monotone profiles in the N  -  1 edges are spectrally stable, whereas the shifted states with non-monotone profiles in the N  -  1 edges are spectrally unstable, the two families intersect at the half-soliton states which are spectrally stable but nonlinearly unstable under the NLS flow. Since the NLS equation on a star graph with shifted states can be reduced to the homogeneous NLS equation on an infinite line, the spectral instability of shifted states is due to the perturbations breaking this reduction. We give a simple argument suggesting that the spectrally stable shifted states in the case of (ii) are nonlinearly unstable under the NLS flow due to the perturbations breaking the reduction to the homogeneous NLS equation.

EphB4 localises to the nucleus of prostate cancer cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mertens-Walker, Inga, E-mail: inga.mertenswalker@qut.edu.au; Australian Prostate Cancer Research Centre—Queensland, Translational Research Institute, 37 Kent Street, Woolloongabba 4102, QLD; Lisle, Jessica E.

2015-04-10

The EphB4 receptor tyrosine kinase is over-expressed in a variety of different epithelial cancers including prostate where it has been shown to be involved in survival, migration and angiogenesis. We report here that EphB4 also resides in the nucleus of prostate cancer cell lines. We used in silico methods to identify a bipartite nuclear localisation signal (NLS) in the extracellular domain and a monopartite NLS sequence in the intracellular kinase domain of EphB4. To determine whether both putative NLS sequences were functional, fragments of the EphB4 sequence containing each NLS were cloned to create EphB4NLS-GFP fusion proteins. Localisation of bothmore » NLS-GFP proteins to the nuclei of transfected cells was observed, demonstrating that EphB4 contains two functional NLS sequences. Mutation of the key amino residues in both NLS sequences resulted in diminished nuclear accumulation. As nuclear translocation is often dependent on importins we confirmed that EphB4 and importin-α can interact. To assess if nuclear EphB4 could be implicated in gene regulatory functions potential EphB4-binding genomic loci were identified using chromatin immunoprecipitation and Lef1 was confirmed as a potential target of EphB4-mediated gene regulation. These novel findings add further complexity to the biology of this important cancer-associated receptor. - Highlights: • The EphB4 protein can be found in the nucleus of prostate cancer cell lines. • EphB4 contains two functional nuclear localisation signals. • Chromatin immunoprecipitation has identified potential genome sequences to which EphB4 binds. • Lef1 is a confirmed target for EphB4-mediated gene regulation.« less

Guidance, Counseling, and Support Services for High School Students with Physical Disabilities: Visual, Hearing, Orthopedic, Neuromuscular, Epilepsy, Chronic Health Conditions. Includes State Resource Directory.

ERIC Educational Resources Information Center

Foster, June C.; And Others

Intended for use by high school guidance personnel, the two volumes provide general information and a resource guide on physical disabilities including visual impairment, hearing impairment, orthopedic handicap, neuromuscular handicap, epilepsy, diabetes, and other chronic health conditions. The first section provides an overview of each of the…

A Sequenced Instructional Program in Physical Education for the Handicapped, Phase III. Producing and Disseminating Demonstration Packages. Final Report.

ERIC Educational Resources Information Center

Carr, Dorothy B.; Avance, Lyonel D.

Presented is a sequenced instructional program in physical education which constitutes the third of a three-phase, 4-year project, funded by Title III, for handicapped children, preschool through high school levels, in the Los Angeles Unified School District. Described are the project setting and the following accomplishments: a curriculum guide…

Comparison of Apple, Epson, IBM, and Other Microcomputers for Applications in Rehabilitation Systems for Persons with Physical Handicaps. Revision D.

ERIC Educational Resources Information Center

Vanderheiden, Gregg C.

The paper analyzes major microcomputer systems and their use in rehabilitative systems for persons with physical handicaps. Four categories of microcomputers are addressed: systems designed for home or school with emphasis on low cost, recreation, and educational software; general purpose microcomputers with applications in a large number of…

75 FR 30373 - Elimination of Form ITA-362P, Information on Articles for Physically or Mentally Handicapped...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-01

... 0625-XA06 Elimination of Form ITA--362P, Information on Articles for Physically or Mentally Handicapped... Trade Administration (ITA) notifies the public that Form ITA-362P, which importers of articles for the... of the Florence Agreement to include duty-free treatment of imports of articles for the use or...

Making Facilities Accessible for the Physically Handicapped. Act. No. 1 of the Public Acts of 1966.

ERIC Educational Resources Information Center

Michigan State Legislature, Lansing.

A physical handicap is defined as an impairment which affects an individual to the extent that special facilities are needed to provide for his safety. Facilities include--(1) the special design of parking lots, building approaches and entrances, (2) stairs, ramps, doors, and multilevel floors, (3) corridors, and (4) rooms with sloping floors,…

36 CFR § 701.6 - Loans of library materials for blind and other physically handicapped persons.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Loans of library materials for... Property LIBRARY OF CONGRESS PROCEDURES AND SERVICES § 701.6 Loans of library materials for blind and other physically handicapped persons. (a) Program. In connection with the Library's program of service under the...

Library Resources for the Blind and Physically Handicapped: A Directory with FY 1998 Statistics on Readership, Circulation, Budget, Staff, and Collections.

ERIC Educational Resources Information Center

Library of Congress, Washington, DC. National Library Service for the Blind and Physically Handicapped.

This directory lists National Library Service for the Blind and Physically Handicapped libraries and machine-lending agencies alphabetically by state. Each entry includes address, phone and fax numbers, e-mail address, World Wide Web site, area served, librarian name, hours, book collection, special collections, assistive devices, special…

Curriculum Guidelines for Teaching Profound and Severely Retarded Students (I.Q. Under 40) Including Those with Physical Handicaps.

ERIC Educational Resources Information Center

Bailey, Constance

1975-01-01

Presented are curriculum guidelines for teaching severely and profoundly retarded students, including students with physical handicaps. In addition to a listing of aims and objectives (such as that the student should analyze the need for space for himself and others), guidelines are provided for the following areas: awareness (of such stimuli as…

Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor.

PubMed

Do, Hyun-Jin; Song, Hyuk; Yang, Heung-Mo; Kim, Dong-Ku; Kim, Nam-Hyung; Kim, Jin-Hoi; Cha, Kwang-Yul; Chung, Hyung-Min; Kim, Jae-Hwan

2006-03-20

We investigated nuclear localization signal (NLS) determinants within the AT-hook and ETS DNA-binding domains of murine Elf3 (mElf3), a member of the subfamily of epithelium-specific ETS transcription factors. Deletion mutants containing the AT-hook, ETS domain or both localized strictly in the nucleus, suggesting that these individual domains contain independent NLS motif(s). Within the AT-hook domain, four basic residues (244KRKR247) were critical for strong NLS activity, and two potent bipartite NLS motifs (236-252 and 249-267) were sufficient for nuclear import of mElf3, although less efficient than the full domain. In addition, one stretch of basic residues (318KKK320) within the ETS domain appears to be essential for mElf3 nuclear localization. Taken together, mElf3 contains multiple NLS motifs, which may function cooperatively to effect efficient nuclear transport.

Hearing aid use and long-term health outcomes: hearing handicap, mental health, social engagement, cognitive function, physical health and mortality

PubMed Central

Dawes, Piers; Cruickshanks, Karen J.; Fischer, Mary E.; Klein, Barbara E.K.; Klein, Ronald; Nondahl, David M.

2016-01-01

Objective To clarify the impact of hearing aids on mental health, social engagement, cognitive function, and physical health outcomes in older adults with hearing impairment. Design We assessed hearing handicap (Hearing Handicap Inventory for the Elderly; HHIE-S), cognition (Mini Mental State Exam, Trail Making, Auditory Verbal Learning, Digit-Symbol Substitution, Verbal Fluency, incidence of cognitive impairment), physical health (SF-12 physical component, basic and instrumental activities of daily living, mortality), social engagement (hours per week spent in solitary activities) and mental health (SF-12 mental component) at baseline, 5 years prior to baseline, and 5 and 11 years after baseline. Study sample Community-dwelling older adults with hearing impairment (N=666) from the Epidemiology of Hearing Loss Study cohort. Results There were no significant differences between hearing aid users and non-users in cognitive, social engagement or mental health outcomes at any time point. Aided HHIE-S was significantly better than unaided HHIE-S. At 11 years hearing aid users had significantly better SF-12 physical health scores (46.2 versus 41.2; p=0.03). There was no difference in incidence of cognitive impairment or mortality. Conclusion There was no evidence that hearing aids promote cognitive function, mental health, or social engagement. Hearing aids may reduce hearing handicap and promote better physical health. PMID:26140300

Arginine-rich cross-linking peptides with different SV40 nuclear localization signal content as vectors for intranuclear DNA delivery.

PubMed

Bogacheva, Mariia; Egorova, Anna; Slita, Anna; Maretina, Marianna; Baranov, Vladislav; Kiselev, Anton

2017-11-01

The major barriers for intracellular DNA transportation by cationic polymers are their toxicity, poor endosomal escape and inefficient nuclear uptake. Therefore, we designed novel modular peptide-based carriers modified with SV40 nuclear localization signal (NLS). Core peptide consists of arginine, histidine and cysteine residues for DNA condensation, endosomal escape promotion and interpeptide cross-linking, respectively. We investigated three polyplexes with different NLS content (10 mol%, 50 mol% and 90 mol% of SV40 NLS) as vectors for intranuclear DNA delivery. All carriers tested were able to condense DNA, to protect it from DNAase I and were not toxic to the cells. We observed that cell cycle arrest by hydroxyurea did not affect transfection efficacy of NLS-modified carriers which we confirmed using quantitative confocal microscopy analysis. Overall, peptide carrier modified with 90 mol% of SV40 NLS provided efficient transfection and nuclear uptake in non-dividing cells. Thus, incorporation of NLS into arginine-rich cross-linking peptides is an adequate approach to the development of efficient intranuclear gene delivery vehicles. Copyright © 2017 Elsevier Ltd. All rights reserved.

Piwi Nuclear Localization and Its Regulatory Mechanism in Drosophila Ovarian Somatic Cells.

PubMed

Yashiro, Ryu; Murota, Yukiko; Nishida, Kazumichi M; Yamashiro, Haruna; Fujii, Kaede; Ogai, Asuka; Yamanaka, Soichiro; Negishi, Lumi; Siomi, Haruhiko; Siomi, Mikiko C

2018-06-19

In Drosophila ovarian somatic cells (OSCs), Piwi represses transposons transcriptionally to maintain genome integrity. Piwi nuclear localization requires the N terminus and PIWI-interacting RNA (piRNA) loading of Piwi. However, the underlying mechanism remains unknown. Here, we show that Importinα (Impα) plays a pivotal role in Piwi nuclear localization and that Piwi has a bipartite nuclear localization signal (NLS). Impα2 and Impα3 are highly expressed in OSCs, whereas Impα1 is the least expressed. Loss of Impα2 or Impα3 forces Piwi to be cytoplasmic, which is rectified by overexpression of any Impα members. Extension of Piwi-NLS with an additional Piwi-NLS leads Piwi to be imported to the nucleus in a piRNA-independent manner, whereas replacement of Piwi-NLS with SV40-NLS fails. Limited proteolysis analysis suggests that piRNA loading onto Piwi triggers conformational change, exposing the N terminus to the environment. These results suggest that Piwi autoregulates its nuclear localization by exposing the NLS to Impα upon piRNA loading. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

NLSdb-major update for database of nuclear localization signals and nuclear export signals.

PubMed

Bernhofer, Michael; Goldberg, Tatyana; Wolf, Silvana; Ahmed, Mohamed; Zaugg, Julian; Boden, Mikael; Rost, Burkhard

2018-01-04

NLSdb is a database collecting nuclear export signals (NES) and nuclear localization signals (NLS) along with experimentally annotated nuclear and non-nuclear proteins. NES and NLS are short sequence motifs related to protein transport out of and into the nucleus. The updated NLSdb now contains 2253 NLS and introduces 398 NES. The potential sets of novel NES and NLS have been generated by a simple 'in silico mutagenesis' protocol. We started with motifs annotated by experiments. In step 1, we increased specificity such that no known non-nuclear protein matched the refined motif. In step 2, we increased the sensitivity trying to match several different families with a motif. We then iterated over steps 1 and 2. The final set of 2253 NLS motifs matched 35% of 8421 experimentally verified nuclear proteins (up from 21% for the previous version) and none of 18 278 non-nuclear proteins. We updated the web interface providing multiple options to search protein sequences for NES and NLS motifs, and to evaluate your own signal sequences. NLSdb can be accessed via Rostlab services at: https://rostlab.org/services/nlsdb/. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Impedance and Otoscopy Screening of Multiply Handicapped Children in School.

ERIC Educational Resources Information Center

Bruns, Janet M.; And Others

1979-01-01

In order to examine the effectiveness of impedance and otoscopic screening in the determination of middle ear abnormalities, 79 physically handicapped, mentally retarded school children (mean age 8 years) were examined. (Author/PHR)

A 1966 SUMMER PROGRAM FOR CHILDREN WITH PHYSICAL, MULTIPLE AND MENTAL HANDICAPS, JUNE 22, 1966 - AUGUST 17, 1966. PROGRAM EVALUATION.

ERIC Educational Resources Information Center

Milwaukee Public Schools, WI.

THIS SUMMER PROGRAM FOR HANDICAPPED CHILDREN WAS DESIGNED TO MEET THE SPECIAL NEEDS OF THESE CHILDREN FOR SOCIALIZATION AND BETTER USE OF LEISURE TIME AND TO PROVIDE DATA WHICH WOULD ENABLE THE DIVISION OF MUNICIPAL RECREATION AND ADULT EDUCATION OF THE MILWAUKEE PUBLIC SCHOOLS TO PLAN FUTURE PROGRAMS. BOTH THE MENTALLY HANDICAPPED CHILDREN'S…

Collapse for the higher-order nonlinear Schrödinger equation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Achilleos, V.; Diamantidis, S.; Frantzeskakis, D. J.

We examine conditions for finite-time collapse of the solutions of the higher-order nonlinear Schr odinger (NLS) equation incorporating third-order dispersion, self-steepening, linear and nonlinear gain and loss, and Raman scattering; this is a system that appears in many physical contexts as a more realistic generalization of the integrable NLS. By using energy arguments, it is found that the collapse dynamics is chiefly controlled by the linear/nonlinear gain/loss strengths. We identify a critical value of the linear gain, separating the possible decay of solutions to the trivial zero-state, from collapse. The numerical simulations, performed for a wide class of initial data,more » are found to be in very good agreement with the analytical results, and reveal long-time stability properties of localized solutions. The role of the higher-order effects to the transient dynamics is also revealed in these simulations.« less

Collapse for the higher-order nonlinear Schrödinger equation

DOE PAGES

Achilleos, V.; Diamantidis, S.; Frantzeskakis, D. J.; ...

2016-02-01

We examine conditions for finite-time collapse of the solutions of the higher-order nonlinear Schr odinger (NLS) equation incorporating third-order dispersion, self-steepening, linear and nonlinear gain and loss, and Raman scattering; this is a system that appears in many physical contexts as a more realistic generalization of the integrable NLS. By using energy arguments, it is found that the collapse dynamics is chiefly controlled by the linear/nonlinear gain/loss strengths. We identify a critical value of the linear gain, separating the possible decay of solutions to the trivial zero-state, from collapse. The numerical simulations, performed for a wide class of initial data,more » are found to be in very good agreement with the analytical results, and reveal long-time stability properties of localized solutions. The role of the higher-order effects to the transient dynamics is also revealed in these simulations.« less

Testing a model of antecedents and consequences of defensive pessimism and self-handicapping in school physical education.

PubMed

Ntoumanis, Nikos; Taylor, Ian M; Standage, Martyn

2010-12-01

There has been very limited research on the use of self-worth protection strategies in the achievement context of school physical education (PE). Thus the aim of the present study was to examine some antecedents and consequences of defensive pessimism and self-handicapping. The sample comprised 534 British pupils (275 females, 259 males) recruited from two schools who responded to established questionnaires. Results of structural equation modelling analysis indicated that self-handicapping and defensive pessimism were positively predicted by fear of failure and negatively predicted by competence valuation. In addition, defensive pessimism was negatively predicted by physical self-concept. In turn, defensive pessimism negatively predicted enjoyment in PE and intentions to participate in future optional PE programmes. Self-handicapping did not predict enjoyment or intentions. Results from multi-sample structural equation modelling showed the specified model to be largely invariant across males and females. The findings indicate that although both strategies aim to protect one's self-worth, some of their antecedents and consequences in PE may differ.

Implementation of an Initial Training Program for New Employees in a Profoundly Mentally and Physically Handicapped Children and Adult Residential Facility.

ERIC Educational Resources Information Center

Hoeft, Linda A.

A project was designed to reduce the turnover of direct care staff in a community-based residential facility that provides a home and educational or adult day training services for 54 severely or profoundly mentally and physically handicapped children and adults. The project sought to reduce total staff turnover by 40%, reduce employee…

38 CFR Appendix B to Subpart E of... - List of Age Distinctions Contained in Statutes and Regulations Governing Federal Financial...

Code of Federal Regulations, 2013 CFR

2013-07-01

..., incurs a disabling illness or injury . . . which results in such child's inability to continue or resume... begin before the person's eighteenth birthday; (2) if the person has a mental or physical handicap, and... attendance age under State law; or (2) his or her 14th birthday and due to physical or mental handicap may...

38 CFR Appendix B to Subpart E of... - List of Age Distinctions Contained in Statutes and Regulations Governing Federal Financial...

Code of Federal Regulations, 2014 CFR

2014-07-01

..., incurs a disabling illness or injury . . . which results in such child's inability to continue or resume... begin before the person's eighteenth birthday; (2) if the person has a mental or physical handicap, and... attendance age under State law; or (2) his or her 14th birthday and due to physical or mental handicap may...

Institute on Outdoor Education for the Handicapped. (Battle Creek Outdoor Education Center, Clear Lake, Dowling, Michigan, June 1969).

ERIC Educational Resources Information Center

Battle Creek Public Schools, MI.

In early June 1969, 55 special education and physical education teachers and experts in outdoor education met for a week at the Battle Creek Public Schools' outdoor education center, Clear Lake Camp (Michigan). The participants shared in the camping activities of children from Battle Creek classes for the physically and mentally handicapped, deaf,…

Development of Audio and Visual Media to Accompany Sequenced Instructional Programs in Physical Education for the Handicapped. Final Report. July 31, 1972.

ERIC Educational Resources Information Center

Avance, Lyonel D.; Carr, Dorothy B.

Presented is the final report of a project to develop and field test audio and visual media to accompany developmentally sequenced activities appropriate for a physical education program for handicapped children from preschool through high school. Brief sections cover the following: the purposes and accomplishments of the project; the population…

Pools for the Handicapped.

ERIC Educational Resources Information Center

American School and University, 1979

1979-01-01

Three institutions in Ohio now stress hydrotherapy and water recreation as important parts of individual educational programs for the handicapped. Specially designed and adapted pools provide freedom of movement and ego building as well as physical education and recreation. (Author)

36 CFR 701.6 - Loans of library materials for blind and other physically handicapped persons.

Code of Federal Regulations, 2011 CFR

2011-07-01

... or private schools where handicapped students are enrolled; however, the students in public or... their educational, vocational, and cultural opportunities in the field of music. Such scores, texts, and...

Educational and Occupational Aspirations and Early Attainment of Black Males and Females.

ERIC Educational Resources Information Center

Epps, Edgar G.; Jackson, Kenneth W.

The effects of school factors on occupational attainment of black students were studied based on data from the 1972 National Longitudinal Study (NLS) and its 1980 followup and the 1980 High School and Beyond (HSB) study and its 1982 followup. The sample sizes were as follows: 319 NLS females, 167 NLS males, 324 HSB females, and 259 HSB males.…

Is oxidative status influenced by dietary carotenoid and physical activity after moult in the great tit (Parus major)?

PubMed

Vaugoyeau, Marie; Decencière, Beatriz; Perret, Samuel; Karadas, Filiz; Meylan, Sandrine; Biard, Clotilde

2015-07-01

In the context of sexual and natural selection, an allocation trade-off for carotenoid pigments may exist because of their obligate dietary origin and their role both in the antioxidant and immune systems and in the production of coloured signals in various taxa, particularly birds. When birds have expended large amounts of carotenoids to feather growth such as after autumn moult, bird health and oxidative status might be more constrained. We tested this hypothesis in a bird species with carotenoid-based plumage colour, by manipulating dietary carotenoids and physical activity, which can decrease antioxidant capacity and increase reactive oxygen metabolite (ROM) concentration. Great tits were captured after moult and kept in aviaries, under three treatments: physical handicap and dietary supplementation with carotenoids, physical handicap and control diet, and no handicap and control diet. We measured plasma composition (antioxidant capacity, ROM concentration, and vitamin A, vitamin E and total carotenoid concentrations), immune system activation (blood sedimentation) and stress response (heterophil/lymphocyte ratio) and predicted that handicap treatment should influence these negatively and carotenoid supplementation positively. Coloration of yellow feathers was also measured. Carotenoid supplementation increased total plasma carotenoid concentration, decreased feather carotenoid chroma and marginally increased ROM concentration. Handicap increased blood sedimentation only in males but had no clear influence on oxidative stress, which contradicted previous studies. Further studies are needed to investigate how physical activity and carotenoid availability might interact and influence oxidative stress outside the moult period, and their combined potential influence on attractiveness and reproductive investment later during the breeding season. © 2015. Published by The Company of Biologists Ltd.

Modulational Instability of Cylindrical and Spherical NLS Equations. Statistical Approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grecu, A. T.; Grecu, D.; Visinescu, Anca

2010-01-21

The modulational (Benjamin-Feir) instability for cylindrical and spherical NLS equations (c/s NLS equations) is studied using a statistical approach (SAMI). A kinetic equation for a two-point correlation function is written and analyzed using the Wigner-Moyal transform. The linear stability of the Fourier transform of the two-point correlation function is studied and an implicit integral form for the dispersion relation is found. This is solved for different expressions of the initial spectrum (delta-spectrum, Lorentzian, Gaussian), and in the case of a Lorentzian spectrum the total growth of the instability is calculated. The similarities and differences with the usual one-dimensional NLS equationmore » are emphasized.« less

Voice handicap in essential tremor: a comparison with normal controls and Parkinson's disease.

PubMed

Louis, Elan D; Gerbin, Marina

2013-01-01

Although voice tremor is one of the most commonly noted clinical features of essential tremor (ET), there are nearly no published data on the handicap associated with it. The Voice Handicap Index (VHI) was self-administered by participants enrolled in a research study at Columbia University Medical Center. The VHI quantifies patients' perceptions of handicap due to voice difficulties. Data from 98 ET cases were compared with data from 100 controls and 85 patients with another movement disorder (Parkinson's disease, PD). Voice tremor was present on examination in 25 (25.5%) ET cases; 12 had mild voice tremor (ETMild VT) and 13 had marked voice tremor (ETMarked VT). VHI scores were higher in ET cases than controls (p = 0.02). VHI scores among ETMarked VT were similar to those of PD cases; both were significantly higher than controls (p<0.001). The three VHI subscale scores (physical, functional, emotional) were highest in ETMarked VT, with values that were similar to those observed in PD. The voice handicap associated with ET had multiple (i.e., physical, functional, and emotional) dimensions. Moreover, ET cases with marked voice tremor on examination had a level of self-reported voice handicap that was similar to that observed in patients with PD.

Help Physically Handicapped Students Achieve Business Skills.

ERIC Educational Resources Information Center

Simon, Judith C.

1980-01-01

Lists suggestions for teachers to prepare them for teaching clerical skills to handicapped students. Specifically discusses teaching typewriting to students with missing fingers or arms and business skills to hearing and visually impaired students. Lists sources of additional information. (JOW)

22 CFR 1510.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

22 CFR 1510.103 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Relations AFRICAN DEVELOPMENT FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

Architectural Considerations for Classrooms for Exceptional Children.

ERIC Educational Resources Information Center

Texas Education Agency, Austin.

Definitions are provided of the following exceptionalities: blind, partially sighted, physically handicapped, minimally brain injured, deaf, educable mentally retarded (primary, junior, and senior high levels), trainable mentally retarded, speech handicapped, and emotionally disturbed. Architectural guidelines specify classroom location, size,…

Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages.

PubMed

Inoue, Takanobu; Iida, Atsuo; Maegawa, Shingo; Sehara-Fujisawa, Atsuko; Kinoshita, Masato

2016-12-01

In this study, we verified nuclear transport activity of an artificial nuclear localization signal (aNLS) in medaka fish (Oryzias latipes). We generated a transgenic medaka strain expresses the aNLS tagged enhanced green fluorescent protein (EGFP) driven by a medaka beta-actin promoter. The aNLS-EGFP was accumulated in the nuclei of somatic tissues and yolk nuclei of oocytes, but undetectable in the spermatozoa. The fluorescent signal was observed from immediately after fertilization by a maternal contribution. Furthermore, male and female pronuclei were visualized in fertilized eggs, and nuclear dynamics of pronuclear fusion and subsequent cleavage were captured by time-lapse imaging. In contrast, SV40NLS exhibited no activity of nuclear transport in early embryos. In conclusion, the aNLS possesses a strong nuclear localization activity and is a useful probe for fluorescent observation of the pronuclei and nuclei in early developmental stage of medaka. © 2016 Japanese Society of Developmental Biologists.

Connections between Narrow Line Seyfert 1 Galaxies and Stellar Black Hole Candidates

NASA Astrophysics Data System (ADS)

Negoro, H.

Connections between narrow line Seyfert 1 galaxies (NLS1s) and black hole candidates are described. It has been pointed out that X-ray properties of NLS1s are simlar to those of stellar black hole candidates (BHCs). It is, however, not clear that NLS1s are corresponding to what `state' in the BHCs. Recently, rapid spectral variations during X-ray flares in a few NLS1s have been discovered using ASCA data. The properties of the spectral variations are very similar to those seen in stellar black hole candidates in the hard state. Such temporal variability accompanying the spectral change has not been recognized in black hole candidates in other states. These and recent theoretical progress based on a time variability model of the BHCs in the hard state imply that the advection plays an important role in the accretion process not only in the BHCs in the hard state, but also in NLS1s.

Nuclear localization of coactivator RAC3 is mediated by a bipartite NLS and importin {alpha}3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yeung, Percy Luk; Zhang, Aihua; Chen, J. Don

2006-09-15

The nuclear receptor coactivator RAC3 (also known as SRC-3/ACTR/AIB1/p/CIP/TRAM-1) belongs to the p160 coactivator family, which are involved in several physiological processes and diseases. Here we have investigated how RAC3 is translocated into the nucleus and show that it is mediated through a bipartite NLS and importin {alpha}3. This bipartite NLS is located within the conserved bHLH domain, and its mutation abolished nuclear localization. The NLS is also sufficient to cause nuclear import of EGFP, and the activity requires basic amino acids within the NLS. RAC3 binds strongly to importin {alpha}3, which also depends on the basic amino acids. Functionally,more » RAC3 cytoplasmic mutant loses its ability to enhance transcription, suggesting that nuclear localization is essential for coactivator function. Together, these results reveal a previous unknown mechanism for nuclear translocation of p160 coactivators and a critical function of the conserved bHLH within the coactivator.« less

Time-dependent spectral renormalization method

NASA Astrophysics Data System (ADS)

Cole, Justin T.; Musslimani, Ziad H.

2017-11-01

The spectral renormalization method was introduced by Ablowitz and Musslimani (2005) as an effective way to numerically compute (time-independent) bound states for certain nonlinear boundary value problems. In this paper, we extend those ideas to the time domain and introduce a time-dependent spectral renormalization method as a numerical means to simulate linear and nonlinear evolution equations. The essence of the method is to convert the underlying evolution equation from its partial or ordinary differential form (using Duhamel's principle) into an integral equation. The solution sought is then viewed as a fixed point in both space and time. The resulting integral equation is then numerically solved using a simple renormalized fixed-point iteration method. Convergence is achieved by introducing a time-dependent renormalization factor which is numerically computed from the physical properties of the governing evolution equation. The proposed method has the ability to incorporate physics into the simulations in the form of conservation laws or dissipation rates. This novel scheme is implemented on benchmark evolution equations: the classical nonlinear Schrödinger (NLS), integrable PT symmetric nonlocal NLS and the viscous Burgers' equations, each of which being a prototypical example of a conservative and dissipative dynamical system. Numerical implementation and algorithm performance are also discussed.

Computational identification of post-translational modification-based nuclear import regulations by characterizing nuclear localization signal-import receptor interaction.

PubMed

Lin, Jhih-Rong; Liu, Zhonghao; Hu, Jianjun

2014-10-01

The binding affinity between a nuclear localization signal (NLS) and its import receptor is closely related to corresponding nuclear import activity. PTM-based modulation of the NLS binding affinity to the import receptor is one of the most understood mechanisms to regulate nuclear import of proteins. However, identification of such regulation mechanisms is challenging due to the difficulty of assessing the impact of PTM on corresponding nuclear import activities. In this study we proposed NIpredict, an effective algorithm to predict nuclear import activity given its NLS, in which molecular interaction energy components (MIECs) were used to characterize the NLS-import receptor interaction, and the support vector regression machine (SVR) was used to learn the relationship between the characterized NLS-import receptor interaction and the corresponding nuclear import activity. Our experiments showed that nuclear import activity change due to NLS change could be accurately predicted by the NIpredict algorithm. Based on NIpredict, we developed a systematic framework to identify potential PTM-based nuclear import regulations for human and yeast nuclear proteins. Application of this approach has identified the potential nuclear import regulation mechanisms by phosphorylation of two nuclear proteins including SF1 and ORC6. © 2014 Wiley Periodicals, Inc.

A set of enhanced green fluorescent protein concatemers for quantitative determination of nuclear localization signal strength.

PubMed

Böhm, Jennifer; Thavaraja, Ramya; Giehler, Susanne; Nalaskowski, Marcus M

2017-09-15

Regulated transport of proteins between nucleus and cytoplasm is an important process in the eukaryotic cell. In most cases, active nucleo-cytoplasmic protein transport is mediated by nuclear localization signal (NLS) and/or nuclear export signal (NES) motifs. In this study, we developed a set of vectors expressing enhanced GFP (EGFP) concatemers ranging from 2 to 12 subunits (2xEGFP to 12xEGFP) for analysis of NLS strength. As shown by in gel GFP fluorescence analysis and αGFP Western blotting, EGFP concatemers are expressed as fluorescent full-length proteins in eukaryotic cells. As expected, nuclear localization of concatemeric EGFPs decreases with increasing molecular weight. By oligonucleotide ligation this set of EGFP concatemers can be easily fused to NLS motifs. After determination of intracellular localization of EGFP concatemers alone and fused to different NLS motifs we calculated the size of a hypothetic EGFP concatemer showing a defined distribution of EGFP fluorescence between nucleus and cytoplasm (n/c ratio = 2). Clear differences of the size of the hypothetic EGFP concatemer depending on the fused NLS motif were observed. Therefore, we propose to use the size of this hypothetic concatemer as quantitative indicator for comparing strength of different NLS motifs. Copyright © 2017 Elsevier Inc. All rights reserved.

Dissection of a nuclear localization signal.

PubMed

Hodel, M R; Corbett, A H; Hodel, A E

2001-01-12

The regulated process of protein import into the nucleus of a eukaryotic cell is mediated by specific nuclear localization signals (NLSs) that are recognized by protein import receptors. This study seeks to decipher the energetic details of NLS recognition by the receptor importin alpha through quantitative analysis of variant NLSs. The relative importance of each residue in two monopartite NLS sequences was determined using an alanine scanning approach. These measurements yield an energetic definition of a monopartite NLS sequence where a required lysine residue is followed by two other basic residues in the sequence K(K/R)X(K/R). In addition, the energetic contributions of the second basic cluster in a bipartite NLS ( approximately 3 kcal/mol) as well as the energy of inhibition of the importin alpha importin beta-binding domain ( approximately 3 kcal/mol) were also measured. These data allow the generation of an energetic scale of nuclear localization sequences based on a peptide's affinity for the importin alpha-importin beta complex. On this scale, a functional NLS has a binding constant of approximately 10 nm, whereas a nonfunctional NLS has a 100-fold weaker affinity of 1 microm. Further correlation between the current in vitro data and in vivo function will provide the foundation for a comprehensive quantitative model of protein import.

Design of an Automated Library Information Storage and Retrieval System for Library of Congress Division for the Blind and Physically Handicapped (DBPH). Final Report.

ERIC Educational Resources Information Center

Systems Architects, Inc., Randolph, MA.

A practical system for producing a union catalog of titles in the collections of the Library of Congress Division for the Blind and Physically Handicapped (DBPH), its regional network, and related agencies from a machine-readable data base is presented. The DBPH organization and operations and the associated regional library network are analyzed.…

A Novel Nuclear Trafficking Module Regulates the Nucleocytoplasmic Localization of the Rabies Virus Interferon Antagonist, P Protein*

PubMed Central

Oksayan, Sibil; Wiltzer, Linda; Rowe, Caitlin L.; Blondel, Danielle; Jans, David A.; Moseley, Gregory W.

2012-01-01

Regulated nucleocytoplasmic transport of proteins is central to cellular function and dysfunction during processes such as viral infection. Active protein trafficking into and out of the nucleus is dependent on the presence within cargo proteins of intrinsic specific modular signals for nuclear import (nuclear localization signals, NLSs) and export (nuclear export signals, NESs). Rabies virus (RabV) phospho (P) protein, which is largely responsible for antagonising the host anti-viral response, is expressed as five isoforms (P1–P5). The subcellular trafficking of these isoforms is thought to depend on a balance between the activities of a dominant N-terminal NES (N-NES) and a distinct C-terminal NLS (C-NLS). Specifically, the N-NES-containing isoforms P1 and P2 are cytoplasmic, whereas the shorter P3–P5 isoforms, which lack the N-NES, are believed to be nuclear through the activity of the C-NLS. Here, we show for the first time that RabV P contains an additional strong NLS in the N-terminal region (N-NLS), which, intriguingly, overlaps with the N-NES. This arrangement represents a novel nuclear trafficking module where the N-NLS is inactive in P1 but becomes activated in P3, concomitant with truncation of the N-NES, to become the principal targeting signal conferring nuclear accumulation. Understanding this unique switch arrangement of overlapping, co-regulated NES/NLS sequences is vital to delineating the critical role of RabV P protein in viral infection. PMID:22700958

Oversight on Education for All Handicapped Children Act, 1980. Oversight on Public Law 94-142, Education for All Handicapped Children Act. Part 2. Hearings before the Subcommittee on the Handicapped of the Committee on Labor and Human Resources, United States Senate, Ninety-Sixth Congress, Second Session (July 29 and 31, and September 10, 1980).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. Senate Committee on Labor and Human Resources.

The document contains proceedings from oversight hearings on the problems and difficulties of implementing P.L. 94-142, the Education for All Handicapped Children Act. Testimonies cover such issues as the failure to carry out effective and proper physical education programs, improper student placement, misapplication of the mainstreaming concept,…

38 CFR 18.437 - Nonacademic services.

Code of Federal Regulations, 2011 CFR

2011-07-01

... handicapped students physical education and athletic activities that are separate or different from those... services and activities in a manner which gives handicapped students an equal opportunity for participation in these services and activities. (2) Nonacademic and extracurricular services and activities may...

5 CFR 1636.103 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FEDERAL RETIREMENT THRIFT INVESTMENT BOARD... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

40 CFR 12.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL NONDISCRIMINATION ON THE BASIS OF HANDICAP..., walks, parking lots, rolling stock or other conveyances, or other real or personal property. Individual with handicaps means any person who has a physical or mental impairment that substantially limits one...

5 CFR 1850.103 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Administrative Personnel OFFICE OF SPECIAL COUNSEL ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

22 CFR 1701.103 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Relations UNITED STATES INSTITUTE OF PEACE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

22 CFR 1701.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Relations UNITED STATES INSTITUTE OF PEACE ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

Telecommunications: A New Horizon for the Handicapped.

ERIC Educational Resources Information Center

Cunningham, Pat; Gose, Joan

The paper describes a computer bulletin board program operated by physically handicapped high school students. Through the bulletin board system, resource people have been contacted, students' written communication and interpersonal relationships have been strengthened, and professional contact has been strengthened. Administrative implications…

Generalized fractional supertrace identity for Hamiltonian structure of NLS-MKdV hierarchy with self-consistent sources

NASA Astrophysics Data System (ADS)

Dong, Huan He; Guo, Bao Yong; Yin, Bao Shu

2016-06-01

In the paper, based on the modified Riemann-Liouville fractional derivative and Tu scheme, the fractional super NLS-MKdV hierarchy is derived, especially the self-consistent sources term is considered. Meanwhile, the generalized fractional supertrace identity is proposed, which is a beneficial supplement to the existing literature on integrable system. As an application, the super Hamiltonian structure of fractional super NLS-MKdV hierarchy is obtained.

Identification and functional characterization of a novel bipartite nuclear localization sequence in ARID1A.

PubMed

Bateman, Nicholas W; Shoji, Yutaka; Conrads, Kelly A; Stroop, Kevin D; Hamilton, Chad A; Darcy, Kathleen M; Maxwell, George L; Risinger, John I; Conrads, Thomas P

2016-01-01

AT-rich interactive domain-containing protein 1A (ARID1A) is a recently identified nuclear tumor suppressor frequently altered in solid tumor malignancies. We have identified a bipartite-like nuclear localization sequence (NLS) that contributes to nuclear import of ARID1A not previously described. We functionally confirm activity using GFP constructs fused with wild-type or mutant NLS sequences. We further show that cyto-nuclear localized, bipartite NLS mutant ARID1A exhibits greater stability than nuclear-localized, wild-type ARID1A. Identification of this undescribed functional NLS within ARID1A contributes vital insights to rationalize the impact of ARID1A missense mutations observed in patient tumors. Copyright © 2015 Elsevier Inc. All rights reserved.

34 CFR 1200.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL COUNCIL ON DISABILITY § 1200.103... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other... body. Individual with handicaps means any person who has a physical or mental impairment that...

12 CFR 794.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... UNION ADMINISTRATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. Handicapped person means any person who has a physical or...

22 CFR 1005.103 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. Handicapped person means any person who has a physical or...

10 CFR 1041.103 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... OF ENERGY (GENERAL PROVISIONS) ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. Handicapped person means any person who has a physical or...

29 CFR 33.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Secretary of Labor ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES..., equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. Individual with handicaps means any person who has a physical or mental impairment that...

12 CFR 606.603 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FARM CREDIT ADMINISTRATION § 606.603..., structures, equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. (f) Individual with handicaps means any person who has a physical or mental impairment...

22 CFR 1005.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Relations INTER-AMERICAN FOUNDATION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. Handicapped person means any person who has a physical or...

34 CFR 104.12 - Reasonable accommodation.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... accommodation to the known physical or mental limitations of an otherwise qualified handicapped applicant or...

34 CFR 104.12 - Reasonable accommodation.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... accommodation to the known physical or mental limitations of an otherwise qualified handicapped applicant or...

34 CFR 104.12 - Reasonable accommodation.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... accommodation to the known physical or mental limitations of an otherwise qualified handicapped applicant or...

34 CFR 104.12 - Reasonable accommodation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... accommodation to the known physical or mental limitations of an otherwise qualified handicapped applicant or...

34 CFR 104.12 - Reasonable accommodation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... accommodation to the known physical or mental limitations of an otherwise qualified handicapped applicant or...

A Point of Discrimination--Public Law 94-142.

ERIC Educational Resources Information Center

Padula, William V.

1979-01-01

The paper points out that because of an incorrect interpretation of the development of learning disabilities, physically handicapped children with learning disabilities are being discriminated against in the provisions of Public Law 94-142, the Education for All Handicapped Children Act. (PHR)

Handbook of Reasonable Accommodation.

ERIC Educational Resources Information Center

Heaton, Sandra M.; And Others

The booklet discusses a basic concept in affirmative action and nondiscrimination for the handicapped, which requires federal agencies to make reasonable accommodation to the physical or mental limitations of a qualified handicapped applicant or employee unless the accommodation would impose an undue hardship on the agency. Reasonable…

Impact of Type I Interferon on the Safety and Immunogenicity of an Experimental Live-Attenuated Herpes Simplex Virus 1 Vaccine in Mice

PubMed Central

Royer, Derek J.; Carr, Meghan M.; Chucair-Elliott, Ana J.; Halford, William P.

2017-01-01

ABSTRACT Viral fitness dictates virulence and capacity to evade host immune defenses. Understanding the biological underpinnings of such features is essential for rational vaccine development. We have previously shown that the live-attenuated herpes simplex virus 1 (HSV-1) mutant lacking the nuclear localization signal (NLS) on the ICP0 gene (0ΔNLS) is sensitive to inhibition by interferon beta (IFN-β) in vitro and functions as a highly efficacious experimental vaccine. Here, we characterize the host immune response and in vivo pathogenesis of HSV-1 0ΔNLS relative to its fully virulent parental strain in C57BL/6 mice. Additionally, we explore the role of type 1 interferon (IFN-α/β) signaling on virulence and immunogenicity of HSV-1 0ΔNLS and uncover a probable sex bias in the induction of IFN-α/β in the cornea during HSV-1 infection. Our data show that HSV-1 0ΔNLS lacks neurovirulence even in highly immunocompromised mice lacking the IFN-α/β receptor. These studies support the translational viability of the HSV-1 0ΔNLS vaccine strain by demonstrating that, while it is comparable to a virulent parental strain in terms of immunogenicity, HSV-1 0ΔNLS does not induce significant tissue pathology. IMPORTANCE HSV-1 is a common human pathogen associated with a variety of clinical presentations ranging in severity from periodic “cold sores” to lethal encephalitis. Despite the consistent failures of HSV subunit vaccines in clinical trials spanning the past 28 years, opposition to live-attenuated HSV vaccines predicated on unfounded safety concerns currently limits their widespread acceptance. Here, we demonstrate that a live-attenuated HSV-1 vaccine has great translational potential. PMID:28122977

Influenza A H3N2 subtype virus NS1 protein targets into the nucleus and binds primarily via its C-terminal NLS2/NoLS to nucleolin and fibrillarin

PubMed Central

2012-01-01

Background Influenza A virus non-structural protein 1 (NS1) is a virulence factor, which is targeted into the cell cytoplasm, nucleus and nucleolus. NS1 is a multi-functional protein that inhibits host cell pre-mRNA processing and counteracts host cell antiviral responses. Previously, we have shown that the NS1 protein of the H3N2 subtype influenza viruses possesses a C-terminal nuclear localization signal (NLS) that also functions as a nucleolar localization signal (NoLS) and targets the protein into the nucleolus. Results Here, we show that the NS1 protein of the human H3N2 virus subtype interacts in vitro primarily via its C-terminal NLS2/NoLS and to a minor extent via its N-terminal NLS1 with the nucleolar proteins, nucleolin and fibrillarin. Using chimeric green fluorescence protein (GFP)-NS1 fusion constructs, we show that the nucleolar retention of the NS1 protein is determined by its C-terminal NLS2/NoLS in vivo. Confocal laser microscopy analysis shows that the NS1 protein colocalizes with nucleolin in nucleoplasm and nucleolus and with B23 and fibrillarin in the nucleolus of influenza A/Udorn/72 virus-infected A549 cells. Since some viral proteins contain NoLSs, it is likely that viruses have evolved specific nucleolar functions. Conclusion NS1 protein of the human H3N2 virus interacts primarily via the C-terminal NLS2/NoLS and to a minor extent via the N-terminal NLS1 with the main nucleolar proteins, nucleolin, B23 and fibrillarin. PMID:22909121

Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Zi Chao; Chook, Yuh Min

Mutations in the proline/tyrosine–nuclear localization signal (PY-NLS) of the Fused in Sarcoma protein (FUS) cause amyotrophic lateral sclerosis (ALS). Here we report the crystal structure of the FUS PY-NLS bound to its nuclear import receptor Karyopherinβ2 (Kapβ2; also known as Transportin). The FUS PY-NLS occupies the structurally invariant C-terminal arch of Kapβ2, tracing a path similar to that of other characterized PY-NLSs. Unlike other PY-NLSs, which generally bind Kapβ2 in fully extended conformations, the FUS peptide is atypical as its central portion forms a 2.5-turn α-helix. The Kapβ2-binding epitopes of the FUS PY-NLS consist of an N-terminal PGKM hydrophobic motif,more » a central arginine-rich α-helix, and a C-terminal PY motif. ALS mutations are found almost exclusively within these epitopes. Each ALS mutation site makes multiple contacts with Kapβ2 and mutations of these residues decrease binding affinities for Kapβ2 (K D for wild-type FUS PY-NLS is 9.5 nM) up to ninefold. Thermodynamic analyses of ALS mutations in the FUS PY-NLS show that the weakening of FUS-Kapβ2 binding affinity, the degree of cytoplasmic mislocalization, and ALS disease severity are correlated.« less

Functional analysis of the C-terminal region of human adenovirus E1A reveals a misidentified nuclear localization signal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cohen, Michael J.; King, Cason R.; Dikeakos, Jimmy D.

The immortalizing function of the human adenovirus 5 E1A oncoprotein requires efficient localization to the nucleus. In 1987, a consensus monopartite nuclear localization sequence (NLS) was identified at the C-terminus of E1A. Since that time, various experiments have suggested that other regions of E1A influence nuclear import. In addition, a novel bipartite NLS was recently predicted at the C-terminal region of E1A in silico. In this study, we used immunofluorescence microscopy and co-immunoprecipitation analysis with importin-α to verify that full nuclear localization of E1A requires the well characterized NLS spanning residues 285–289, as well as a second basic patch situatedmore » between residues 258 and 263 ({sup 258}RVGGRRQAVECIEDLLNEPGQPLDLSCKRPRP{sup 289}). Thus, the originally described NLS located at the C-terminus of E1A is actually a bipartite signal, which had been misidentified in the existing literature as a monopartite signal, altering our understanding of one of the oldest documented NLSs. - Highlights: • Human adenovirus E1A is localized to the nucleus. • The C-terminus of E1A contains a bipartite nuclear localization signal (NLS). • This signal was previously misidentified to be a monopartite NLS. • Key basic amino acid residues within this sequence are highly conserved.« less

Structural determination of importin alpha in complex with beak and feather disease virus capsid nuclear localization signal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Patterson, Edward I.; EH Graham Centre for Agricultural Innovation; Dombrovski, Andrew K.

2013-09-06

Highlights: •Circovirus capsid proteins contain large nuclear localization signals (NLS). •A method of nuclear import has not been elucidated. •Beak and feather disease virus (BFDV) capsid NLS was crystallized with importin α. •The structure showed BFDV NLS binding to the major site of importin α. •Result shows implications for mechanism of nuclear transport for all circoviruses. -- Abstract: Circoviruses represent a rapidly increasing genus of viruses that infect a variety of vertebrates. Replication requires shuttling viral molecules into the host cell nucleus, a process facilitated by capsid-associated protein (Cap). Whilst a nuclear localization signal (NLS) has been shown to mediatemore » nuclear translocation, the mode of nuclear transport remains to be elucidated. To better understand this process, beak and feather disease virus (BFDV) Cap NLS was crystallized with nuclear import receptor importin-α (Impα). Diffraction yielded structural data to 2.9 Å resolution, and the binding site on both Impα and BFDV Cap NLS were well resolved. The binding mechanism for the major site is likely conserved across circoviruses as supported by the similarity of NLSs in circovirus Caps. This finding illuminates a crucial step for infection of host cells by this viral family, and provides a platform for rational drug design against the binding interface.« less

The NLS-Based Nonlinear Grey Multivariate Model for Forecasting Pollutant Emissions in China.

PubMed

Pei, Ling-Ling; Li, Qin; Wang, Zheng-Xin

2018-03-08

The relationship between pollutant discharge and economic growth has been a major research focus in environmental economics. To accurately estimate the nonlinear change law of China's pollutant discharge with economic growth, this study establishes a transformed nonlinear grey multivariable (TNGM (1, N )) model based on the nonlinear least square (NLS) method. The Gauss-Seidel iterative algorithm was used to solve the parameters of the TNGM (1, N ) model based on the NLS basic principle. This algorithm improves the precision of the model by continuous iteration and constantly approximating the optimal regression coefficient of the nonlinear model. In our empirical analysis, the traditional grey multivariate model GM (1, N ) and the NLS-based TNGM (1, N ) models were respectively adopted to forecast and analyze the relationship among wastewater discharge per capita (WDPC), and per capita emissions of SO₂ and dust, alongside GDP per capita in China during the period 1996-2015. Results indicated that the NLS algorithm is able to effectively help the grey multivariable model identify the nonlinear relationship between pollutant discharge and economic growth. The results show that the NLS-based TNGM (1, N ) model presents greater precision when forecasting WDPC, SO₂ emissions and dust emissions per capita, compared to the traditional GM (1, N ) model; WDPC indicates a growing tendency aligned with the growth of GDP, while the per capita emissions of SO₂ and dust reduce accordingly.

Reorientational versus Kerr dark and gray solitary waves using modulation theory.

PubMed

Assanto, Gaetano; Marchant, T R; Minzoni, Antonmaria A; Smyth, Noel F

2011-12-01

We develop a modulation theory model based on a Lagrangian formulation to investigate the evolution of dark and gray optical spatial solitary waves for both the defocusing nonlinear Schrödinger (NLS) equation and the nematicon equations describing nonlinear beams, nematicons, in self-defocusing nematic liquid crystals. Since it has an exact soliton solution, the defocusing NLS equation is used as a test bed for the modulation theory applied to the nematicon equations, which have no exact solitary wave solution. We find that the evolution of dark and gray NLS solitons, as well as nematicons, is entirely driven by the emission of diffractive radiation, in contrast to the evolution of bright NLS solitons and bright nematicons. Moreover, the steady nematicon profile is nonmonotonic due to the long-range nonlocality associated with the perturbation of the optic axis. Excellent agreement is obtained with numerical solutions of both the defocusing NLS and nematicon equations. The comparisons for the nematicon solutions raise a number of subtle issues relating to the definition and measurement of the width of a dark or gray nematicon.

Handicaps No Hindrance with Horses

ERIC Educational Resources Information Center

Seeley, Colleen

1974-01-01

A horseback riding program, sponsored by 4-H members for handicapped children in Michigan's Genesse County, has proven physically and emotionally veneficial for the children. All therapeutic exercises were performed with the approval of the child's physician and therapist. Plans for expanding the program are being considered. (AG)

Access: Exceptional Children and Technology.

ERIC Educational Resources Information Center

North Carolina State Dept. of Public Instruction, Raleigh. Div. for Exceptional Children.

The Exceptional Children and New Technology project sought to meet the instructional needs of physically handicapped, emotionally disturbed, learning disabled, and mentally handicapped children through the use of computer technology. The goals of the project were to test the instructional value of adaptive/assistive devices with exceptional…

22 CFR 711.103 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OVERSEAS PRIVATE INVESTMENT..., walks, parking lots, rolling stock or other conveyances, or other real or personal property. Historic... appropriate State or local government body. Individual with handicaps means any person who has a physical or...

22 CFR 711.103 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE OVERSEAS PRIVATE INVESTMENT..., walks, parking lots, rolling stock or other conveyances, or other real or personal property. Historic... appropriate State or local government body. Individual with handicaps means any person who has a physical or...

13 CFR 136.103 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE SMALL BUSINESS ADMINISTRATION § 136... any portion of buildings, structures, equipment, roads, walks, parking lots, rolling stock or other conveyances, or other real or personal property. Individual with handicaps means any person who has a physical...

Physiotherapy in Ordinary Schools.

ERIC Educational Resources Information Center

Davies, Jean; Hunt, Agnes

1980-01-01

A program to provide physiotherapy to mainstreamed physically handicapped English school children (from preschool through high school) is described. Begun in 1978, the once a week service has increased the independence of the children served and brought a better understanding of the handicapped students' capabilities to their teachers. (PHR)

7 CFR 15b.3 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., such diseases and conditions as orthopedic, visual, speech, and hearing impairments; cerebral palsy... (1974), the Education for All Handicapped Children Act of 1975, Public Law 94-142, 89 Stat. 773 (1975... property. (i) Handicapped person means any person who has a physical or mental impairment which...

Special Vocational Program for Handicapped Youth and Adults. Revised Edition.

ERIC Educational Resources Information Center

Jernberg, Donald E.

The Salina Area Vocational Technical School has a special vocational program which provides selected vocational training courses for students with physical, emotional, or intellectual handicaps. The program offers vocational counseling, placement services, work evaluation, job readiness training, and vocational training tryouts. Vocational…

Multi-Handicapped Blind Persons Can Work.

ERIC Educational Resources Information Center

Rusalem, Herbert; Richterman, Harold

The demonstration project assessed an innovative approach to the provision of remunerative work for evaluation, training, and employment purposes in sheltered workshops for 291 blind individuals who also were limited by vocationally significant intellectual, physical, emotional, and/or social disabilities. The multiply handicapped subgroup of the…

15 CFR 8b.12 - Reasonable accommodation.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Section 8b.12 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION AGAINST THE HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Employment... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...

15 CFR 8b.12 - Reasonable accommodation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Section 8b.12 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION AGAINST THE HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Employment... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...

15 CFR 8b.12 - Reasonable accommodation.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Section 8b.12 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION AGAINST THE HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Employment... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...

15 CFR 8b.12 - Reasonable accommodation.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Section 8b.12 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION AGAINST THE HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Employment... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...

15 CFR 8b.12 - Reasonable accommodation.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Section 8b.12 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION AGAINST THE HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Employment... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...

PRISE Reporter. Volume 12, 1980-81.

ERIC Educational Resources Information Center

PRISE Reporter, 1981

1981-01-01

The document consists of six issues of the "PRISE (Pennsylvania Resources and Information Center for Special Education) Reporter" which cover issues and happenings in the education of the mentally retarded, learning disabled, emotionally disturbed, physically handicapped, visually handicapped, and speech/hearing impaired. Lead articles include the…

Teaching Biology to Visually Handicapped Students. Resource Manual.

ERIC Educational Resources Information Center

Ricker, Kenneth S.

This resource manual presents numerous techniques for adapting science activities to the visually handicapped student, applicable to introductory biology courses in which microscopes are used extensively in the laboratory. Chapters include information on the following: alternative microscopic viewing techniques, physical models, tactile diagrams,…

15 CFR 8c.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.3 Definitions..., walks, parking lots, rolling stock or other conveyances, or other real or personal property. Individual with handicaps means any person who has a physical or mental impairment that substantially limits one...

Handicapped Individuals Services and Training Act. Hearing before the Subcommittee on Select Education of the Committee on Education and Labor, House of Representatives, Ninety-Seventh Congress, Second Session on H.R. 6820 (St. Paul, Minnesota and Loretto, Minnesota).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House Committee on Education and Labor.

This document is a transcript of hearings concerning the Handicapped Individuals Services and Training Act that would provide funds for operation of the Helen Keller National Center for Deaf-Blind Youths and Adults, the Vinland National Center for Health-Sports and Physical Fitness for Handicapped Individuals, and other projects and services for…

Refraction of dispersive shock waves

NASA Astrophysics Data System (ADS)

El, G. A.; Khodorovskii, V. V.; Leszczyszyn, A. M.

2012-09-01

We study a dispersive counterpart of the classical gas dynamics problem of the interaction of a shock wave with a counter-propagating simple rarefaction wave, often referred to as the shock wave refraction. The refraction of a one-dimensional dispersive shock wave (DSW) due to its head-on collision with the centred rarefaction wave (RW) is considered in the framework of the defocusing nonlinear Schrödinger (NLS) equation. For the integrable cubic nonlinearity case we present a full asymptotic description of the DSW refraction by constructing appropriate exact solutions of the Whitham modulation equations in Riemann invariants. For the NLS equation with saturable nonlinearity, whose modulation system does not possess Riemann invariants, we take advantage of the recently developed method for the DSW description in non-integrable dispersive systems to obtain main physical parameters of the DSW refraction. The key features of the DSW-RW interaction predicted by our modulation theory analysis are confirmed by direct numerical solutions of the full dispersive problem.

Time-Reversal Generation of Rogue Waves

NASA Astrophysics Data System (ADS)

Chabchoub, Amin; Fink, Mathias

2014-03-01

The formation of extreme localizations in nonlinear dispersive media can be explained and described within the framework of nonlinear evolution equations, such as the nonlinear Schrödinger equation (NLS). Within the class of exact NLS breather solutions on a finite background, which describe the modulational instability of monochromatic wave trains, the hierarchy of rational solutions localized in both time and space is considered to provide appropriate prototypes to model rogue wave dynamics. Here, we use the time-reversal invariance of the NLS to propose and experimentally demonstrate a new approach to constructing strongly nonlinear localized waves focused in both time and space. The potential applications of this time-reversal approach include remote sensing and motivated analogous experimental analysis in other nonlinear dispersive media, such as optics, Bose-Einstein condensates, and plasma, where the wave motion dynamics is governed by the NLS.

Orbital stability and energy estimate of ground states of saturable nonlinear Schrödinger equations with intensity functions in R2

NASA Astrophysics Data System (ADS)

Lin, Tai-Chia; Wang, Xiaoming; Wang, Zhi-Qiang

2017-10-01

Conventionally, the existence and orbital stability of ground states of nonlinear Schrödinger (NLS) equations with power-law nonlinearity (subcritical case) can be proved by an argument using strict subadditivity of the ground state energy and the concentration compactness method of Cazenave and Lions [4]. However, for saturable nonlinearity, such an argument is not applicable because strict subadditivity of the ground state energy fails in this case. Here we use a convexity argument to prove the existence and orbital stability of ground states of NLS equations with saturable nonlinearity and intensity functions in R2. Besides, we derive the energy estimate of ground states of saturable NLS equations with intensity functions using the eigenvalue estimate of saturable NLS equations without intensity function.

Narrow-Line Seyfert 1 Galaxies and their place in the Universe

NASA Astrophysics Data System (ADS)

Foschini, L.; Colpi, M.; Gallo, L.; Grupe, D.; Komossa, S.; Leighly, K.; Mathur, S.

In 1978, Davidson and Kinman wrote about Markarian 359: "This unusual object merits further observations...". In 1985, Osterbrock and Pogge defined a new class of active galactic nuclei (AGN), named Narrow-Line Seyfert 1 (NLS1). Twenty-five years later, NLS1s still continue to intrigue and bewilder. NLS1s manifest extreme behaviour at all wavelengths. They exhibit the most extreme X-ray variability seen in radio-quiet AGN, the most intense optical FeII emission, and high rates of star formation. In general, their characteristics are consistent of AGNs with relatively low mass black holes accreting close to the Eddington rate. The 2009 Fermi Gamma-ray Space Telescope discovery of high-energy (E>100 MeV) gamma rays in a handful of NLS1s has established the existence of relativistic jets in these systems -- a fact previously hinted at by the flat radio spectrum and high brightness temperature seen in some objects. Since NLS1 are generally hosted by spirals, this poses some intriguing questions on the galaxy evolution and on how relativistic jets are generated. It is therefore time for the broad community to come together and discuss what we have discovered in the last quarter century and lay the foundation for future work. Workshop Topics: * Central engine: BH mass, accretion disk, BLR/NLR, jet * Host galaxy: morphology, star formation, merging history * NLS1 in the Universe: comparison with other types of AGN, surveys/statistics, formation/merging, cosmological evolution

The NLS-Based Nonlinear Grey Multivariate Model for Forecasting Pollutant Emissions in China

PubMed Central

Pei, Ling-Ling; Li, Qin

2018-01-01

The relationship between pollutant discharge and economic growth has been a major research focus in environmental economics. To accurately estimate the nonlinear change law of China’s pollutant discharge with economic growth, this study establishes a transformed nonlinear grey multivariable (TNGM (1, N)) model based on the nonlinear least square (NLS) method. The Gauss–Seidel iterative algorithm was used to solve the parameters of the TNGM (1, N) model based on the NLS basic principle. This algorithm improves the precision of the model by continuous iteration and constantly approximating the optimal regression coefficient of the nonlinear model. In our empirical analysis, the traditional grey multivariate model GM (1, N) and the NLS-based TNGM (1, N) models were respectively adopted to forecast and analyze the relationship among wastewater discharge per capita (WDPC), and per capita emissions of SO2 and dust, alongside GDP per capita in China during the period 1996–2015. Results indicated that the NLS algorithm is able to effectively help the grey multivariable model identify the nonlinear relationship between pollutant discharge and economic growth. The results show that the NLS-based TNGM (1, N) model presents greater precision when forecasting WDPC, SO2 emissions and dust emissions per capita, compared to the traditional GM (1, N) model; WDPC indicates a growing tendency aligned with the growth of GDP, while the per capita emissions of SO2 and dust reduce accordingly. PMID:29517985

Vygotskian Tradition in the Psychological Study of Handicapped, Particularly Deaf Children.

ERIC Educational Resources Information Center

Knox, Jane; Kozulin, Alex

The paper reviews theories of Lev Vygotsky, founder of the Soviet school of cognitive developmental psychology and an architect of Soviet defectology, the discipline concerned with physically and mentally handicapped children. Three of his basic concepts are explained: (1) "cultural" versus "natural" mental functions; (2)…

Rights Handbook for Physically Handicapped Children: Child Advocacy Project.

ERIC Educational Resources Information Center

Minch, Janet; And Others

The handbook is intended to help parents of handicapped children understand and use Massachusetts' laws and federal legislation to obtain their children's rights to education, training, medical services, and tax and social security benefits. Guidelines regarding education concern such topics as the Bartley-Daly Law; class placement; home tutoring;…

Related Service Personnel's Resource Guide for Supporting Programs for Emotionally Handicapped Students.

ERIC Educational Resources Information Center

Indiana State Dept. of Education, Indianapolis. Div. of Special Education.

The guide provides an information resource for related and supportive services personnel (e.g., school nurse, physical therapist, speech language pathologist) in their interactions with emotionally handicapped (EH) students. Following a definition of EH students, the first of six brief chapters discusses student characteristics, presents three…

Camp Courageous of Iowa Staff Manual.

ERIC Educational Resources Information Center

Camp Courageous of Iowa, Monticello.

Designed as a useful and practical tool for the staff at Camp Courageous of Iowa, a year-round residential camp serving all handicapped individuals, the manual outlines safety rules for camp activities, characteristics of the mentally and physically handicapped, and a general description of the camp and its objectives. Contents of the manual…

A Family Oriented Enrichment Program for Handicapped Infants.

ERIC Educational Resources Information Center

Furuno, Setsu; O'Reilly, Kitty

This report describes a program for handicapped infants which emphasizes early treatment as a prime means of preventing more serious physical and behavioral problems later in life. Also, the program focuses on the entire family of the developmentally disabled, including infant, parents, and siblings. Program objectives include (1) increased…

Fostering Creativity in Handicapped Children.

ERIC Educational Resources Information Center

Sherrill, Claudine

Of the nine long range goals of adapted physical education, creative thinking and moving has received the least attention. However, this goal may be more important than others in helping handicapped children self-actualize through sports, dance, and aquatics. This paper defines behaviors in the creative process and describes several assessment…

The Resource Specialist in the Community College.

ERIC Educational Resources Information Center

Ribera, Gilbert J.

Described are results of a survey of California State Community College services for physically handicapped students in which 74 college coordinators and 102 handicapped students completed questionnaires. Findings are said to indicate that most of the state's community colleges did not employ a full time staff person to coordinate services for…

34 CFR Appendix A to Part 100 - Federal Financial Assistance to Which These Regulations Apply

Code of Federal Regulations, 2012 CFR

2012-07-01

.... Grants for research and demonstrations relating to physical education or recreation for handicapped...). 5. Loan service of captioned films and educational media; research on, and production and.... 1321). 7. Research and related activities in education of handicapped children (20 U.S.C. 1441). 8...

34 CFR Appendix A to Part 100 - Federal Financial Assistance to Which These Regulations Apply

Code of Federal Regulations, 2014 CFR

2014-07-01

.... Grants for research and demonstrations relating to physical education or recreation for handicapped...). 5. Loan service of captioned films and educational media; research on, and production and.... 1321). 7. Research and related activities in education of handicapped children (20 U.S.C. 1441). 8...

34 CFR Appendix A to Part 100 - Federal Financial Assistance to Which These Regulations Apply

Code of Federal Regulations, 2013 CFR

2013-07-01

.... Grants for research and demonstrations relating to physical education or recreation for handicapped...). 5. Loan service of captioned films and educational media; research on, and production and.... 1321). 7. Research and related activities in education of handicapped children (20 U.S.C. 1441). 8...

Examining Young Children's Perceptions of Handicaps.

ERIC Educational Resources Information Center

Ludlow, Barbara L.

The purposes of this study were to assess the attitudes of preschoolers toward two types of handicapping conditions (mental retardation and physical disabilities), and to explore the underlying social reasoning used by young children to formulate/support their perceptions. Sixteen 3- and 4-year-old boys and girls were presented with two…

Information Prosthetics for the Handicapped. Final Report.

ERIC Educational Resources Information Center

Papert, Seymour A.

The paper describes the successful use of the LOGO System (an interactive graphics, computer based learning environment) with 12 severely physically handicapped adolescents with cerebral palsy. Five goals were set forth concerning the opportunity to work with microcomputers in interesting ways, the assessment of the students' spatial competence,…

Science for Handicapped Children.

ERIC Educational Resources Information Center

Jones, Alan V.

This book consists of science activities for 11- to 16-year-old children who have physical disabilities. Part 1 presents information for teachers, parents, curriculum planners, and others who are interested in incorporating some science into the curriculum of the handicapped child. It outlines reasons for doing science, gives a suggested concept…

Structure-based nuclear import mechanism of histones H3 and H4 mediated by Kap123

DOE Office of Scientific and Technical Information (OSTI.GOV)

An, Sojin; Yoon, Jungmin; Kim, Hanseong

Kap123, a major karyopherin protein of budding yeast, recognizes the nuclear localization signals (NLSs) of cytoplasmic histones H3 and H4 and translocates them into the nucleus during DNA replication. Mechanistic questions include H3- and H4-NLS redundancy toward Kap123 and the role of the conserved diacetylation of cytoplasmic H4 (K5ac and K12ac) in Kap123-mediated histone nuclear translocation. Here, we report crystal structures of full-length Kluyveromyces lactis Kap123 alone and in complex with H3- and H4-NLSs. Structures reveal the unique feature of Kap123 that possesses two discrete lysine-binding pockets for NLS recognition. Structural comparison illustrates that H3- and H4-NLSs share at leastmore » one of two lysine-binding pockets, suggesting that H3- and H4-NLSs are mutually exclusive. Additionally, acetylation of key lysine residues at NLS, particularly H4-NLS diacetylation, weakens the interaction with Kap123. These data support that cytoplasmic histone H4 diacetylation weakens the Kap123-H4-NLS interaction thereby facilitating histone Kap123-H3-dependent H3:H4/Asf1 complex nuclear translocation.« less

An insertion in the methyltransferase domain of P. falciparum trimethylguanosine synthase harbors a classical nuclear localization signal.

PubMed

Babar, Prasad H; Dey, Vishakha; Jaiswar, Praveen; Patankar, Swati

Many Plasmodium falciparum proteins do not share homology with, and are generally longer than their respective orthologs. This, to some extent, can be attributed to insertions. Here, we studied a P. falciparum RNA hypermethylase, trimethylguanosine synthase (PfTGS1) that harbors a 76 amino acid insertion in its methyltransferase domain. Bioinformatics analysis revealed that this insertion was present in TGS1 orthologs from other Plasmodium species as well. Interestingly, a classical nuclear localization signal (NLS) was predicted in the insertions of primate parasite TGS1 proteins. To check whether these predicted NLS are functional, we developed an in vivo heterologous system using S. cerevisiae. The predicted NLS when fused to dimeric GFP were able to localize the fusion protein to the nucleus in yeast indicating that it is indeed recognized by the yeast nuclear import machinery. We further showed that the PfTGS1 NLS binds to P. falciparum importin-α in vitro, confirming that the NLS is also recognized by the P. falciparum classical nuclear import machinery. Thus, in this study we report a novel function of the insertion in PfTGS1. Copyright © 2016 Elsevier B.V. All rights reserved.

From nonlinear Schrödinger hierarchy to some (2+1)-dimensional nonlinear pseudodifferential equations

NASA Astrophysics Data System (ADS)

Yang, Xiao; Du, Dianlou

2010-08-01

The Poisson structure on CN×RN is introduced to give the Hamiltonian system associated with a spectral problem which yields the nonlinear Schrödinger (NLS) hierarchy. The Hamiltonian system is proven to be Liouville integrable. Some (2+1)-dimensional equations including NLS equation, Kadomtesev-Petviashvili I (KPI) equation, coupled KPI equation, and modified Kadomtesev-Petviashvili (mKP) equation, are decomposed into Hamilton flows via the NLS hierarchy. The algebraic curve, Abel-Jacobi coordinates, and Riemann-Jacobi inversion are used to obtain the algebrogeometric solutions of these equations.

Computation of Nonlinear Backscattering Using a High-Order Numerical Method

NASA Technical Reports Server (NTRS)

Fibich, G.; Ilan, B.; Tsynkov, S.

2001-01-01

The nonlinear Schrodinger equation (NLS) is the standard model for propagation of intense laser beams in Kerr media. The NLS is derived from the nonlinear Helmholtz equation (NLH) by employing the paraxial approximation and neglecting the backscattered waves. In this study we use a fourth-order finite-difference method supplemented by special two-way artificial boundary conditions (ABCs) to solve the NLH as a boundary value problem. Our numerical methodology allows for a direct comparison of the NLH and NLS models and for an accurate quantitative assessment of the backscattered signal.

Subcutaneous blood flow in psoriasis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klemp, P.

1985-03-01

The simultaneously recorded disappearance rates of /sup 133/xe from subcutaneous adipose tissue in the crus were studied in 10 patients with psoriasis vulgaris using atraumatic labeling of the tissue in lesional skin (LS) areas and symmetrical, nonlesional skin (NLS) areas. Control experiments were performed bilaterally in 10 younger, healthy subjects. The subcutaneous washout rate constant was significantly higher in LS, 0.79 +/- 0.05 min-1 x 10(2) compared to the washout rate constant of NLS, 0.56 +/- 0.07 min-1. 10(2), or the washout rate constant in the normal subjects, 0.46 +/- 0.17 min-1 x 10(2). The mean washout rate constant inmore » NLS was 25% higher than the mean washout rate constant in the normal subjects. The difference was, however, not statistically significant. Differences in the washout rate constants might be due to abnormal subcutaneous tissue-to-blood partition (lambda) in the LS--and therefore not reflecting the real differences in the subcutaneous blood flow (SBF). The lambda for /sup 133/Xe was therefore measured--using a double isotope washout method (/sup 133/Xe and (/sup 131/I)antipyrine)--in symmetrical sites of the lateral crus in LS and NLS of 10 patients with psoriasis vulgaris and in 10 legs of normal subjects. In LS the lambda was 4.52 +/- 1.67 ml/g, which was not statistically different from that of NLS, 5.25 +/- 2.19 ml/g, nor from that of normal subcutaneous tissue, 4.98 +/- 1.04 ml/g. Calculations of the SBF using the obtained lambda values gave a significantly higher SBF in LS, 3.57 +/- 0.23 ml/100 g/min, compared to SBF in the NLS, 2.94 +/- 0.37 ml/100 g/min. There was no statistically significant difference between SBF in NLS and SBF in the normal subjects. The increased SBF in LS of psoriatics might be a secondary phenomenon to an increased heat loss in the lesional skin.« less

[Sport as integration factor of the physically handicapped in our society].

PubMed

Labronici, R H; Cunha, M C; Oliveira, A D; Gabbai, A A

2000-12-01

The objective of this study was to make use of sports as a rehabilitation method, as well as to assess the physical, psychological, and social aspects of those present some physical handicap, particularly those who have some kind of chronic disease and are no longer taking part in any rehabilitation program. Thirty handicapped people were evaluated: fifteen started with basketball and fifteen with swimming, according either to the specific preference of each one of them or to the degree and kind of physical impairment. They were submmited to the following evaluations: clinical examination, physiotherapy assessment, social interview and use of the Rivermead Social Scale, functional classification of the sport, use of the Barthel and Rivermead Functional Scales, and the psychological profile test (POMS). After two years, no relevant change in the moving evolution of the athletes were reported. Concerning the POMS psychological test, both basketball and swimming groups presented with high vigor and low depression levels. Considering the social aspects, both groups presented substantial improvement, specially regarding their relationship to one person or more people and also in the everyday activities (be it social, leisure, or domestic), thus leading them to better social integration. This essay shows that sport can bring people who are physically impaired a better social integration and physical conditions.

Rogue waves in multiphase solutions of the focusing nonlinear Schrödinger equation

NASA Astrophysics Data System (ADS)

Bertola, Marco; El, Gennady A.; Tovbis, Alexander

2016-10-01

Rogue waves appearing on deep water or in optical fibres are often modelled by certain breather solutions of the focusing nonlinear Schrödinger (fNLS) equation which are referred to as solitons on finite background (SFBs). A more general modelling of rogue waves can be achieved via the consideration of multiphase, or finite-band, fNLS solutions of whom the standard SFBs and the structures forming due to their collisions represent particular, degenerate, cases. A generalized rogue wave notion then naturally enters as a large-amplitude localized coherent structure occurring within a finite-band fNLS solution. In this paper, we use the winding of real tori to show the mechanism of the appearance of such generalized rogue waves and derive an analytical criterion distinguishing finite-band potentials of the fNLS equation that exhibit generalized rogue waves.

One pot synthesis of highly luminescent polyethylene glycol anchored carbon dots functionalized with a nuclear localization signal peptide for cell nucleus imaging.

PubMed

Yang, Lei; Jiang, Weihua; Qiu, Lipeng; Jiang, Xuewei; Zuo, Daiying; Wang, Dongkai; Yang, Li

2015-04-14

Strong blue fluorescent polyethylene glycol (PEG) anchored carbon nitride dots (CDs@PEG) with a high quantum yield (QY) of 75.8% have been synthesized by a one step hydrothermal treatment. CDs with a diameter of ca. 6 nm are well dispersed in water and present a graphite-like structure. Photoluminescence (PL) studies reveal that CDs display excitation-dependent behavior and are stable under various test conditions. Based on the as-prepared CDs, we designed novel cell nucleus targeting imaging carbon dots functionalized with a nuclear localization signal (NLS) peptide. The favourable biocompatibilities of CDs and NLS modified CDs (NLS-CDs) are confirmed by in vitro cytotoxicity assays. Importantly, intracellular localization experiments in MCF7 and A549 cells demonstrate that NLS-CDs could be internalized in the nucleus and show blue light, which indicates that CDs may serve as cell nucleus imaging probes.

Asymptotic expansions and solitons of the Camassa-Holm - nonlinear Schrödinger equation

NASA Astrophysics Data System (ADS)

Mylonas, I. K.; Ward, C. B.; Kevrekidis, P. G.; Rothos, V. M.; Frantzeskakis, D. J.

2017-12-01

We study a deformation of the defocusing nonlinear Schrödinger (NLS) equation, the defocusing Camassa-Holm NLS, hereafter referred to as CH-NLS equation. We use asymptotic multiscale expansion methods to reduce this model to a Boussinesq-like equation, which is then subsequently approximated by two Korteweg-de Vries (KdV) equations for left- and right-traveling waves. We use the soliton solution of the KdV equation to construct approximate solutions of the CH-NLS system. It is shown that these solutions may have the form of either dark or antidark solitons, namely dips or humps on top of a stable continuous-wave background. We also use numerical simulations to investigate the validity of the asymptotic solutions, study their evolution, and their head-on collisions. It is shown that small-amplitude dark and antidark solitons undergo quasi-elastic collisions.

A comparison of idiopathic hypersomnia and narcolepsy-cataplexy using self report measures and sleep diary data.

PubMed Central

Bruck, D; Parkes, J D

1996-01-01

Eighteen patients with idiopathic hypersomnia (IH) were compared with 50 patients with the narcoleptic syndrome of cataplexy and daytime sleepiness (NLS) using self report questionnaires and a diary of sleep/wake patterns. The IH group reported more consolidated nocturnal sleep, a lower propensity to nap, greater refreshment after naps, and a greater improvement in excessive daytime sleepiness since onset than the NLS group. In IH, the onset of excessive daytime sleepiness was predominantly associated with familial inheritance or a viral illness. Two variable--number of reported awakenings during nocturnal sleep and the reported change in sleepiness since onset--provided maximum discrimination between the IH and NLS groups. Confusional arousals, extended naps or nocturnal sleep, autonomic nervous system dysfunction, low ratings of medication effectiveness, or side effects of medication were not associated differentially with either IH or NLS. PMID:8778267

Instructional Basics: Oppelt Standard Method of Therapeutic and Recreational Ice Skating.

ERIC Educational Resources Information Center

Oppelt, Kurt

Detailed in the booklet is the standard ice skating method and considered are the benefits of therapeutic ice skating for the handicapped and aged. Values for the mentally retarded and physically handicapped are seen to include physiological (such as increased flexibility and improved posture), psychological (including satifaction and enhanced…

Dance for Students with Orthopedic Conditions--Popular, Square, Folk, Modern, [and] Ballet.

ERIC Educational Resources Information Center

Harris, Cordelia Graves

1979-01-01

Preparation for teaching dance to orthopedically handicapped students is discussed in addition to specific suggestions for teaching popular dance, square dance, folk dance, modern dance, and ballet. Dancing is seen as a way to give orthopedically handicapped students physical education success without competition. Recommended music for each type…

Project on Recreation and Fitness for the Mentally Retarded.

ERIC Educational Resources Information Center

Hamilton City School District, OH.

All educable (IQ 50 to 75) and trainable (IQ 30 to 50) mentally handicapped and orthopedically handicapped children (16,000) in special education classes in Hamilton, Ohio, participated in a physical education program of vigorous movement and varied indoor and outdoor activities. Two full time instructors, 80 volunteers, and a part time secretary…

Activities of Daily Living Curriculum for Handicapped Adults. Materials Development Center Reprint #20.

ERIC Educational Resources Information Center

Wisconsin Univ. - Stout, Menomonie. Dept. of Rehabilitation and Manpower Services. Materials Development Center.

Designed for use in group and shelter homes, this curriculum is intended to impart the necessary skills for independent living. It should also better prepare mentally and physically handicapped individuals with the training required to handle the responsibilities accompanying competitive employment. These fourteen courses are included: money…

Design Guidelines: Study of Handicapped Accessibility in South Carolina State Parks.

ERIC Educational Resources Information Center

South Carolina State Dept. of Parks, Recreation, and Tourism, Columbia. Div. of Engineering and Planning.

The publication provides guidelines for the design of new facilities or rehabilitation of existing facilities to accommodate physically handicapped persons in the South Carolina State Parks system. The guidelines are also recommended for use in regional, special district, county, and municipal parks within the state. The guidelines were developed…

Feeding Management of a Child with a Handicap: A Guide for Professionals.

ERIC Educational Resources Information Center

Smith, Mary Ann Harvey; And Others

Intended for professionals from a variety of disciplines (such as nutrition, dentistry, nursing, occupational and physical therapy, speech pathology, social work, and education), the guide presents information on feeding problems of children with handicaps. Part I, which traces the development of feeding, considers in detail normal development and…

Social Studies in the Mainstreamed Classroom, K-6.

ERIC Educational Resources Information Center

Ochoa, Anna S.; Shuster, Susan K.

A guide is designed for elementary school teachers with handicapped students in their classrooms. Seven sections comprise the document. Section I discusses the implications of mainstreaming for classroom teachers. Discussed are 1) the physical and social isolation of the handicapped students in the 1960s; 2) the impact of Public Law 94-142 which…

Creative Movement Classes for Visually Handicapped Children in a Public School Setting

ERIC Educational Resources Information Center

Resnick, Rose

1973-01-01

To counteract the lack of healthful physical activities offered for visually handicapped children in San Francisco public schools, a creative movement program was implemented for eight girls and four boys, 6 to 20 years of age, who were blind or partially sighted, and ranged in intelligence from retarded to bright. (MC)

Fractures in Older Persons with Mental Handicap.

ERIC Educational Resources Information Center

Jancar, J.

1989-01-01

Examination of case records of 731 residents (age 40 and over) in British hospitals for persons with mental handicap found that 55 males and 62 females had sustained 1 or more fractures. The resident's age, physical and mental states, causes of fractures, and bones involved were studied, as were additional diseases, disorders, or drugs associated…

Mastery Learning of Stunts and Tumbling Activities for the Mentally Retarded.

ERIC Educational Resources Information Center

Chambless, Jim R.; And Others

Intended for physical education teachers of the handicapped, teacher educators at teacher training institutions, and research workers in areas of the handicapped, the document reports the effects of a theoretical model of teaching psychomotor tasks on the motor behavior of mentally retarded children. Chapter 1 introduces a psychomotor teaching…

Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α.

PubMed

Delaforge, Elise; Milles, Sigrid; Bouvignies, Guillaume; Bouvier, Denis; Boivin, Stephane; Salvi, Nicola; Maurin, Damien; Martel, Anne; Round, Adam; Lemke, Edward A; Jensen, Malene Ringkjøbing; Hart, Darren J; Blackledge, Martin

2015-12-09

Influenza A RNA polymerase complex is formed from three components, PA, PB1, and PB2. PB2 is independently imported into the nucleus prior to polymerase reconstitution. All crystallographic structures of the PB2 C-terminus (residues 536-759) reveal two globular domains, 627 and NLS, that form a tightly packed heterodimer. The molecular basis of the affinity of 627-NLS for importins remained unclear from these structures, apparently requiring large-scale conformational changes prior to importin binding. Using a combination of solution-state NMR, small-angle neutron scattering, small-angle X-ray scattering (SAXS), and Förster resonance energy transfer (FRET), we show that 627-NLS populates a temperature-dependent dynamic equilibrium between closed and open states. The closed state is stabilized by a tripartite salt bridge involving the 627-NLS interface and the linker, that becomes flexible in the open state, with 627 and NLS dislocating into a highly dynamic ensemble. Activation enthalpies and entropies associated with the rupture of this interface were derived from simultaneous analysis of temperature-dependent chemical exchange saturation transfer measurements, revealing a strong temperature dependence of both open-state population and exchange rate. Single-molecule FRET and SAXS demonstrate that only the open-form is capable of binding to importin α and that, upon binding, the 627 domain samples a dynamic conformational equilibrium in the vicinity of the C-terminus of importin α. This intrinsic large-scale conformational flexibility therefore enables 627-NLS to bind importin through conformational selection from a temperature-dependent equilibrium comprising both functional forms of the protein.

Side-to-side differences in lower extremity biomechanics during multi-directional jump landing in volleyball athletes.

PubMed

Sinsurin, Komsak; Srisangboriboon, Sarun; Vachalathiti, Roongtiwa

2017-07-01

Side-to-side differences of lower extremities may influence the likelihood of injury. Moreover, adding the complexity of jump-landing direction would help to explain lower extremity control during sport activities. The aim was to determine the effects of limb dominance and jump-landing direction on lower extremity biomechanics. Nineteen female volleyball athletes participated. Both dominant limbs (DLs) and non-dominant limbs (NLs) were examined in single-leg jump-landing tests in four directions, including forward (0°), diagonal (30° and 60°), and lateral (90°) directions. Kinematic marker trajectories and ground reaction forces were collected using a 10 camera Vicon system and an AMTI force plate. Repeated measures ANOVA (2 × 4, limb × direction) was used to analyse. The finding showed that, at peak vertical GRF, a significant interaction of limb dominance and direction effects was found in the hip flexion angle and lower extremity joint kinetics (p < .05). NLs and DLs exhibited significantly different strategies while landing in various directions. Significantly higher increase of ankle dorsiflexion angle was observed in lateral direction compared to other directions for both DLs and NLs (p < .05). Increasingly using ankle dorsiflexion was observed from the forward to the lateral direction for both DLs and NLs. However, NLs and DLs preferentially used different strategies of joint moment organization to respond to similar VGRFs in various directions. The response pattern of DLs might not be effective and may expose DLs to a higher injury risk, especially with regard to landing with awkward posture compared with NLs.

The R35 residue of the influenza A virus NS1 protein has minimal effects on nuclear localization but alters virus replication through disrupting protein dimerization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lalime, Erin N.; Pekosz, Andrew, E-mail: apekosz@jhsph.edu

The influenza A virus NS1 protein has a nuclear localization sequence (NLS) in the amino terminal region. This NLS overlaps sequences that are important for RNA binding as well as protein dimerization. To assess the significance of the NS1 NLS on influenza virus replication, the NLS amino acids were individually mutated to alanines and recombinant viruses encoding these mutations were rescued. Viruses containing NS1 proteins with mutations at R37, R38 and K41 displayed minimal changes in replication or NS1 protein nuclear localization. Recombinant viruses encoding NS1 R35A were not recovered but viruses containing second site mutations at position D39 inmore » addition to the R35A mutation were isolated. The mutations at position 39 were shown to partially restore NS1 protein dimerization but had minimal effects on nuclear localization. These data indicate that the amino acids in the NS1 NLS region play a more important role in protein dimerization compared to nuclear localization. - Highlights: • Mutations were introduced into influenza NS1 NLS1. • NS1 R37A, R38A, K41A viruses had minimal changes in replication and NS1 localization. • Viruses from NS1 R35A rescue all contained additional mutations at D39. • NS1 R35A D39X mutations recover dimerization lost in NS1 R35A mutations. • These results reaffirm the importance of dimerization for NS1 protein function.« less

Improved liver R2* mapping by pixel-wise curve fitting with adaptive neighborhood regularization.

PubMed

Wang, Changqing; Zhang, Xinyuan; Liu, Xiaoyun; He, Taigang; Chen, Wufan; Feng, Qianjin; Feng, Yanqiu

2018-08-01

To improve liver R2* mapping by incorporating adaptive neighborhood regularization into pixel-wise curve fitting. Magnetic resonance imaging R2* mapping remains challenging because of the serial images with low signal-to-noise ratio. In this study, we proposed to exploit the neighboring pixels as regularization terms and adaptively determine the regularization parameters according to the interpixel signal similarity. The proposed algorithm, called the pixel-wise curve fitting with adaptive neighborhood regularization (PCANR), was compared with the conventional nonlinear least squares (NLS) and nonlocal means filter-based NLS algorithms on simulated, phantom, and in vivo data. Visually, the PCANR algorithm generates R2* maps with significantly reduced noise and well-preserved tiny structures. Quantitatively, the PCANR algorithm produces R2* maps with lower root mean square errors at varying R2* values and signal-to-noise-ratio levels compared with the NLS and nonlocal means filter-based NLS algorithms. For the high R2* values under low signal-to-noise-ratio levels, the PCANR algorithm outperforms the NLS and nonlocal means filter-based NLS algorithms in the accuracy and precision, in terms of mean and standard deviation of R2* measurements in selected region of interests, respectively. The PCANR algorithm can reduce the effect of noise on liver R2* mapping, and the improved measurement precision will benefit the assessment of hepatic iron in clinical practice. Magn Reson Med 80:792-801, 2018. © 2018 International Society for Magnetic Resonance in Medicine. © 2018 International Society for Magnetic Resonance in Medicine.

The role of nuclear localization signal in parvovirus life cycle.

PubMed

Liu, Peng; Chen, Shun; Wang, Mingshu; Cheng, Anchun

2017-04-14

Parvoviruses are small, non-enveloped viruses with an approximately 5.0 kb, single-stranded DNA genome. Usually, the parvovirus capsid gene contains one or more nuclear localization signals (NLSs), which are required for guiding the virus particle into the nucleus through the nuclear pore. However, several classical NLSs (cNLSs) and non-classical NLSs (ncNLSs) have been identified in non-structural genes, and the ncNLSs can also target non-structural proteins into the nucleus. In this review, we have summarized recent research findings on parvovirus NLSs. The capsid protein of the adeno-associated virus has four potential nuclear localization sequences, named basic region 1 (BR), BR2, BR3 and BR4. BR3 was identified as an NLS by fusing it with green fluorescent protein. Moreover, BR3 and BR4 are required for infectivity and virion assembly. In Protoparvovirus, the canine parvovirus has a common cNLS located in the VP1 unique region, similar to parvovirus minute virus of mice (MVM) and porcine parvovirus. Moreover, an ncNLS is found in the C-terminal region of MVM VP1/2. Parvovirus B19 also contains an ncNLS in the C-terminal region of VP1/2, which is essential for the nuclear transport of VP1/VP2. Approximately 1 or 2 cNLSs and 1 ncNLS have been reported in the non-structural protein of bocaviruses. Understanding the role of the NLS in the process of parvovirus infection and its mechanism of nuclear transport will contribute to the development of therapeutic vaccines and novel antiviral medicines.

[Sports for handicapped persons between the text and its implementation].

PubMed

Negra Mahjoub, Samia; Djait, Salwa; Mahjoub, Rached; Turki, Ilhem

2003-05-01

The sports activities must occupy a paramount place in the schooling of the handicapped pupils. Indeed in addition to the development of the physical capacities, the sports activity has a positive impact on the development of the capacities of the communication and thus the integration of the pupil handicapped in his educational, family and social circle and on progress of psychomotor acquisitions. The national plan of education and the law N94-10 of August 3, 1994 relating to the sports activity in educational and university circle took part in the development and the promotion of the sports activities in the educational structures. This measurement, applied in the centers of specialized education, did not deny concerned the handicapped pupils provided education for in the colleges and normal colleges. What are the causes? Does there exist a remedy? How to introduce the sport for handicapped into the normal establishments?

Voice handicap and health-related quality of life after treatment for small laryngeal carcinoma.

PubMed

Killguss, Helen; Gottwald, Frank; Haderlein, Tino; Maier, Andreas; Rosanowski, Frank; Iro, Heinrich; Psychogios, Georgios; Schuster, Maria

2011-01-01

Treatment of small carcinoma of the larynx may lead to voice handicap and restricted quality of life. The relationship between the two is revealed. Sixty-five patients aged 62.1 ± 10.0 years rated their voice handicap and quality of life after treatment of T1 (n = 35) or T2 (n = 30) laryngeal carcinoma during regular out-patient examinations. For the self-assessment of the voice, the Voice Handicap Index (VHI) and the disease-independent Short Form-36 Health Survery (SF-36) questionnaires were used. Voice handicap (total score 38.9 ± 26.0) did not differ in the two tested groups, T1 and T2, and the data of SF-36 (physical score 43.0 ± 10.7; mental score 50.2 ± 9.1) showed significant differences for the mental score. Patients rated their voice handicap worse than healthy persons did after treatment of laryngeal carcinoma. VHI and SF-36 data were strongly correlated. Voice handicap is significantly related to the quality of life, especially affecting the mental domain. Thus, the rehabilitation of voice disorders should have a beneficial impact on quality of life. Copyright © 2010 S. Karger AG, Basel.

[Visually-impaired adolescents' interpersonal relationships at school].

PubMed

Bezerra, Camilla Pontes; Pagliuca, Lorita Marlena Freitag

2007-09-01

This study describes the school environment and how interpersonal relationships are conducted in view of the needs of visually handicapped adolescents. Data were collected through observations of the physical environment of two schools in Fortaleza, Ceara, Brazil, with the support of a checklist, in order to analyze the existence of obstacles. Four visually handicapped adolescents from 14 to 20 years of age were interviewed. Conclusions were that the obstacles that hamper the free locomotion, communication, and physical and social interaction of the blind--or people with other eye disorders--during their activities at school are numerous.

On the structure of critical energy levels for the cubic focusing NLS on star graphs

NASA Astrophysics Data System (ADS)

Adami, Riccardo; Cacciapuoti, Claudio; Finco, Domenico; Noja, Diego

2012-05-01

We provide information on a non-trivial structure of phase space of the cubic nonlinear Schrödinger (NLS) on a three-edge star graph. We prove that, in contrast to the case of the standard NLS on the line, the energy associated with the cubic focusing Schrödinger equation on the three-edge star graph with a free (Kirchhoff) vertex does not attain a minimum value on any sphere of constant L2-norm. We moreover show that the only stationary state with prescribed L2-norm is indeed a saddle point.

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moriyama, Tetsuji; Sangel, Percival; Yamaguchi, Hiroki

2015-07-03

Tripartite motif-containing 28 (TRIM28) is a transcription regulator, which forms a repressor complex containing heterochromatin protein 1 (HP1). Here, we report identification of a nuclear localization signal (NLS) within the 462-494 amino acid region of TRIM28 that overlaps with its HP1 binding site, HP1 box. GST-pulldown experiments revealed the interaction of the arginine-rich TRIM28 NLS with various importin α subtypes (α1, α2 and α4). In vitro transport assay demonstrated that nuclear localization of GFP-TRIM28 NLS is mediated by importin αs, in conjunction with importin β1 and Ran. Further, we demonstrated that HP1 and importin αs compete for binding to TRIM28. Together,more » our findings suggest that importin α has an essential role in the nuclear delivery and preferential HP1 interaction of TRIM28. - Highlights: • TRIM28 contains an NLS within the 462-494 amino acid region. • The nuclear import of TRIM28 is mediated by importin α/importin β1. • TRIM28 NLS overlaps with HP1 Box. • HP1 and importin α compete for binding to TRIM28.« less

Morbillivirus nucleoprotein possesses a novel nuclear localization signal and a CRM1-independent nuclear export signal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sato, Hiroki; Masuda, Munemitsu; Miura, Ryuichi

2006-08-15

Morbilliviruses, which belong to the Mononegavirales, replicate its RNA genome in the cytoplasm of the host cell. However, they also form characteristic intranuclear inclusion bodies, consisting of nucleoprotein (N), in infected cells. To analyze the mechanisms of nucleocytoplasmic transport of N protein, we characterized the nuclear localization (NLS) and nuclear export (NES) signals of canine distemper virus (CDV) N protein by deletion mutation and alanine substitution of the protein. The NLS has a novel leucine/isoleucine-rich motif (TGILISIL) at positions 70-77, whereas the NES is composed of a leucine-rich motif (LLRSLTLF) at positions 4-11. The NLS and NES of the Nmore » proteins of other morbilliviruses, that is, measles virus (MV) and rinderpest virus (RPV), were also analyzed. The NLS of CDV-N protein is conserved at the same position in MV-N protein, whereas the NES of MV-N protein is located in the C-terminal region. The NES of RPV-N protein is also located at the same position as CDV-N protein, whereas the NLS motif is present not only at the same locus as CDV-N protein but also at other sites. Interestingly, the nuclear export of all these N proteins appears to proceed via a CRM1-independent pathway.« less

Nuclear localization signal regulates porcine circovirus type 2 capsid protein nuclear export through phosphorylation.

PubMed

Hou, Qiang; Hou, Shaohua; Chen, Qing; Jia, Hong; Xin, Ting; Jiang, Yitong; Guo, Xiaoyu; Zhu, Hongfei

2018-02-15

The open reading frame 2 (ORF2) of Porcine circovirus type 2 (PCV2) encodes the major Capsid (Cap) protein, which self-assembles into virus-like particle (VLP) of similar morphology to the PCV2 virion and accumulates in the nucleus through the N-terminal arginine-rich nuclear localization signal (NLS). In this study, PCV2 Cap protein and its derivates were expressed via the baculovirus expression system, and the cellular localization of the recombinant proteins were investigated using anti-Cap mAb by imaging flow cytometry. Analysis of subcellular localization of Cap protein and its variants demonstrated that NLS mediated Cap protein nuclear export as well as nuclear import, and a phosphorylation site (S17) was identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the NLS domain to regulate Cap protein nuclear export. Phosphorylation of NLS regulating the PCV2 Cap protein nuclear export was also demonstrated in PK15 cells by fluorescence microscopy. Moreover, the influence of Rep and Rep' protein on Cap protein subcellular localization was investigated in PK15 cells. Phosphorylation of NLS regulating Cap protein nuclear export provides more detailed knowledge of the PCV2 viral life cycle. Copyright © 2018 Elsevier B.V. All rights reserved.

Improving the Functional Utility and Effectiveness of Classroom Services for Students with Profound Multiple Handicaps.

ERIC Educational Resources Information Center

Green, Carolyn W.; And Others

1986-01-01

A staff supervision and classroom management program to increase involvement in functional task activities (e.g., self-help, leisure, social/communication) was implemented in three classes serving 19 students (ages 11-36) with profound mental and physical handicaps. Large increases in functional task involvement and smaller increases in student…

The Impact of Childhood Disability on Black and White Families.

ERIC Educational Resources Information Center

Breslau, Naomi

Home interviews were given to 369 families of children (3-18 years old) with cystic fibrosis, cerebral palsy, myelodysplasia, and multiple physical handicaps to determine the impact of childhood disability on families. Results were compared with those of 465 control families who had one or more non-handicapped children in that age range. A cross…

Project Volunteer Power: Volunteers Serving the Job Needs of the Handicapped. Final Report.

ERIC Educational Resources Information Center

President's Committee on Employment of the Handicapped, Washington, DC.

The report covers the activities, results, and lessons learned in a project to test a new approach in developing greater opportunities in employment of the physically handicapped, mentally retarded, and mentally restored by harnessing the resources of leading volunteer women's organizations at the local level on a practical, planned basis. Project…

Effects of Support on the Attitude of the Primary Caregiver of a Child with Down Syndrome.

ERIC Educational Resources Information Center

Heritage, Jeannette G.; And Others

Parents of children with mental or physical handicaps often experience overwhelming feelings of stress and anxiety. Research indicates that support for the primary caregiver can help alleviate some stressors involved in raising a child with a handicap. This study examined how level of support affects feelings of satisfaction the primary caregiver…

THE ELEMENTARY SCHOOL CHILD AND HIS POSTURE PATTERNS.

ERIC Educational Resources Information Center

DAVIES, EVELYN A.

A CHILD'S POSTURE PATTERNS MAY LEAD TO AN ADULT'S PHYSICAL HANDICAP. THE MAIN THEME OF THIS BOOK IS TO SERVE AS A GUIDE FOR THE ELEMENTARY TEACHER OR PARENT IN THE DETECTION AND UNDERSTANDING OF DEVIATIONS FROM THE NORMAL POSTURE PATTERNS WHILE THE CHILD IS SITTING, STANDING, OR MOVING ABOUT SO AS TO PREVENT FUTURE HANDICAPPING CONDITIONS.…

Study of Man-Machine Communications Systems for Disabled Persons (The Handicapped). Volume V. Final Report.

ERIC Educational Resources Information Center

Kafafian, Haig

Instructions are given for teaching severely physically and/or neurologically handicapped students to use the 14-key Cybertype man-machine communications system, an electric writing machine with a simplified keyboard to enable persons with limited motor ability or coordination to communicate in written form. Explained are the various possible…

Study of Man-Machine Communications Systems for Disabled Persons (The Handicapped). Volume VII. Final Report.

ERIC Educational Resources Information Center

Kafafian, Haig

Teaching instructions, lesson plans, and exercises are provided for severely physically and/or neurologically handicapped persons learning to use the Cybertype electric writing machine with a tongue-body keyboard. The keyboard, which has eight double-throw toggle switches and a three-position state-selector switch, is designed to be used by…

Birding: An Introduction to Ornithological Delights for Blind and Physically Handicapped Individuals.

ERIC Educational Resources Information Center

Cylke, Frank Kurt, Ed.

The booklet provides resources for blind and visually handicapped individuals interested in birding as a hobby. It describes a midwestern group of visually impaired birders and discusses the importance and variety of bird sounds. Listed are a selection of recorded and braille books available on birding, sources of commercial recordings of bird…

Auxiliary Aids and Services for Postsecondary Students with Handicaps. Higher Education's Obligations under Section 504.

ERIC Educational Resources Information Center

Office for Civil Rights (ED), Washington, DC.

This pamphlet discusses Section 504 of the Rehabilitation Act of 1973, which prohibits discrimination on the basis of physical or mental handicap by any program or activity receiving federal financial assistance. The regulation applies to all recipients of Department of Education funding, with the focus of this pamphlet including colleges,…

Handicapped Students in the Danish Educational System.

ERIC Educational Resources Information Center

Ministry of Education, Copenhagen (Denmark).

The educational policy of Denmark and the educational system which has evolved from this policy are described. The policy states that everyone has a right to the same access to education and training, regardless of sex, social origins, geographic origins, and physical or mental handicap; and all public education is free of charge from the age of 5…

Assessment and Evaluation in Adapted Physical Education.

ERIC Educational Resources Information Center

Kennedy, Charlotte I.; Bundschuh, Ernie

Intended for physical education teachers, the booklet describes informal and formal methods for evaluating handicapped children to determine whether they can participate in a regular physical education program with nonhandicapped students, in a regular physical education program with modification, or in a specially designed physical education…

46 CFR 153.482 - Stripping quantities and interim standards for Category C NLS tanks on ships built before July 1...

Code of Federal Regulations, 2013 CFR

2013-10-01

... LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.482 Stripping quantities and interim standards for Category C NLS tanks on...

46 CFR 153.482 - Stripping quantities and interim standards for Category C NLS tanks on ships built before July 1...

Code of Federal Regulations, 2012 CFR

2012-10-01

... LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.482 Stripping quantities and interim standards for Category C NLS tanks on...

46 CFR 153.482 - Stripping quantities and interim standards for Category C NLS tanks on ships built before July 1...

Code of Federal Regulations, 2014 CFR

2014-10-01

... LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.482 Stripping quantities and interim standards for Category C NLS tanks on...

46 CFR 153.482 - Stripping quantities and interim standards for Category C NLS tanks on ships built before July 1...

Code of Federal Regulations, 2011 CFR

2011-10-01

... LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.482 Stripping quantities and interim standards for Category C NLS tanks on...

46 CFR 153.482 - Stripping quantities and interim standards for Category C NLS tanks on ships built before July 1...

Code of Federal Regulations, 2010 CFR

2010-10-01

... LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.482 Stripping quantities and interim standards for Category C NLS tanks on...

Nuclear transport of the Neurospora crassa NIT-2 transcription factor is mediated by importin-α.

PubMed

Bernardes, Natália E; Takeda, Agnes A S; Dreyer, Thiago R; Cupertino, Fernanda B; Virgilio, Stela; Pante, Nelly; Bertolini, Maria Célia; Fontes, Marcos R M

2017-12-06

The Neurospora crassa NIT-2 transcription factor belongs to the GATA transcription factor family and plays a fundamental role in the regulation of nitrogen metabolism. Because NIT-2 acts by accessing DNA inside the nucleus, understanding the nuclear import process of NIT-2 is necessary to characterize its function. Thus, in the present study, NIT-2 nuclear transport was investigated using a combination of biochemical, cellular, and biophysical methods. A complemented strain that produced an sfGFP-NIT-2 fusion protein was constructed, and nuclear localization assessments were made under conditions that favored protein translocation to the nucleus. Nuclear translocation was also investigated using HeLa cells, which showed that the putative NIT-2 nuclear localization sequence (NLS; 915 TISSKRQRRHSKS 927 ) was recognized by importin-α and that subsequent transport occurred via the classical import pathway. The interaction between the N. crassa importin-α (NcImpα) and the NIT-2 NLS was quantified with calorimetric assays, leading to the observation that the peptide bound to two sites with different affinities, which is typical of a monopartite NLS sequence. The crystal structure of the NcImpα/NIT-2 NLS complex was solved and revealed that the NIT-2 peptide binds to NcImpα with the major NLS-binding site playing a primary role. This result contrasts other recent studies that suggested a major role for the minor NLS-binding site in importin-α from the α2 family, indicating that both sites can be used for different cargo proteins according to specific metabolic requirements. © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

Nuclear localisation of 53BP1 is regulated by phosphorylation of the nuclear localisation signal.

PubMed

von Morgen, Patrick; Lidak, Tomas; Horejsi, Zuzana; Macurek, Libor

2018-06-01

Repair of damaged DNA is essential for maintaining genomic stability. TP53-binding protein 1 (53BP1) plays an important role in repair of the DNA double-strand breaks. Nuclear localisation of 53BP1 depends on importin β and nucleoporin 153, but the type and location of 53BP1 nuclear localisation signal (NLS) have yet to be determined. Here, we show that nuclear import of 53BP1 depends on two basic regions, namely 1667-KRK-1669 and 1681-KRGRK-1685, which are both needed for importin binding. Lysine 1667 is essential for interaction with importin and its substitution to arginine reduced nuclear localisation of 53BP1. Furthermore, we have found that CDK1-dependent phosphorylation of 53BP1 at S1678 impairs importin binding during mitosis. Phosphorylation-mimicking mutant S1678D showed reduced nuclear localisation, suggesting that phosphorylation of the NLS interferes with nuclear import of the 53BP1 CONCLUSIONS: We show that 53BP1 contains a classical bipartite NLS 1666-GKRKLITSEEERSPAKRGRKS-1686, which enables the importin-mediated nuclear transport of 53BP1. Additionally, we found that posttranslational modification within the NLS region can regulate 53BP1 nuclear import. Our results indicate that integrity of the NLS is important for 53BP1 nuclear localisation. Precise mapping of the NLS will facilitate further studies on the effect of posttranslational modifications and somatic mutations on the nuclear localisation 53BP1 and DNA repair. © 2018 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hirano, Hidemi; Matsuura, Yoshiyuki, E-mail: matsuura.yoshiyuki@d.mbox.nagoya-u.ac.jp

Highlights: {yields} MAL has a bipartite NLS that binds to Imp{alpha} in an extended conformation. {yields} Mutational analyses verified the functional significance of MAL-Imp{alpha} interactions. {yields} Induced folding and NLS-masking by G-actins inhibit nuclear import of MAL. -- Abstract: The coordination of cytoskeletal actin dynamics with gene expression reprogramming is emerging as a crucial mechanism to control diverse cellular processes, including cell migration, differentiation and neuronal circuit assembly. The actin-binding transcriptional coactivator MAL (also known as MRTF-A/MKL1/BSAC) senses G-actin concentration and transduces Rho GTPase signals to serum response factor (SRF). MAL rapidly shuttles between the cytoplasm and the nucleus inmore » unstimulated cells but Rho-induced depletion of G-actin leads to MAL nuclear accumulation and activation of transcription of SRF:MAL-target genes. Although the molecular and structural basis of actin-regulated nucleocytoplasmic shuttling of MAL is not understood fully, it is proposed that nuclear import of MAL is mediated by importin {alpha}/{beta} heterodimer, and that G-actin competes with importin {alpha}/{beta} for the binding to MAL. Here we present structural, biochemical and cell biological evidence that MAL has a classical bipartite nuclear localization signal (NLS) in the N-terminal 'RPEL' domain containing Arg-Pro-X-X-X-Glu-Leu (RPEL) motifs. The NLS residues of MAL adopt an extended conformation and bind along the surface groove of importin-{alpha}, interacting with the major- and minor-NLS binding sites. We also present a crystal structure of wild-type MAL RPEL domain in complex with five G-actins. Comparison of the importin-{alpha}- and actin-complexes revealed that the binding of G-actins to MAL is associated with folding of NLS residues into a helical conformation that is inappropriate for importin-{alpha} recognition.« less

Clinical significance of liver histology on outcomes in biliary atresia.

PubMed

Webb, Nicholas L; Jiwane, Ashish; Ooi, Chee Y; Nightinghale, Scott; Adams, Susan E; Krishnan, Usha

2017-03-01

Biliary atresia (BA) literature has focussed on the relationship between age at Kasai procedure (KP) and post-KP outcomes. This study primarily examines post-KP outcomes including, 6-month normalisation of bilirubin, 5-year native liver survival (NLS), development of portal hypertension (PHT) and incidence of ascending cholangitis at a single tertiary paediatric centre in Australia. The study also evaluated prognostic factors which may influence these aforementioned outcomes. Retrospective chart review of all BA cases between 1999 and 2014. Age at KP, liver biopsy results, use of ursodeoxycholic acid or prophylactic antibiotics and occurrence of PHT and ascending cholangitis post-KP were recorded and related to the primary post-KP outcome measures. BA was diagnosed in 29 patients. Twenty-four of 29 patients underwent KP. Median age at KP was 68 days (29-104). Fourteen of 24 (58.3%) had bridging fibrosis and 5 of 24 (20.8%) had cirrhosis at time of KP. Median follow-up was 8.4 years (2.08-15.58 years). Bilirubin normalisation within 6 months occurred in 7 of 24 (29.2%) patients and 5-year NLS was 45.8% (11/24). Fourteen of 24 (58.3%) had PHT and 18 of 24 (75%) patients had ascending cholangitis post-KP. Absence of bridging fibrosis in liver histology at KP was the only factor to be significantly associated with improved 5-year NLS. None of the other variables examined had a significant association with either 5-year NLS or bilirubin normalisation by 6 months. Five-year NLS in this series was 45.8%. Absence of bridging fibrosis at time of KP was the only factor significantly associated with improved 5-year NLS. © 2016 Paediatrics and Child Health Division (The Royal Australasian College of Physicians).

Classification of homoclinic rogue wave solutions of the nonlinear Schrödinger equation

NASA Astrophysics Data System (ADS)

Osborne, A. R.

2014-01-01

Certain homoclinic solutions of the nonlinear Schrödinger (NLS) equation, with spatially periodic boundary conditions, are the most common unstable wave packets associated with the phenomenon of oceanic rogue waves. Indeed the homoclinic solutions due to Akhmediev, Peregrine and Kuznetsov-Ma are almost exclusively used in scientific and engineering applications. Herein I investigate an infinite number of other homoclinic solutions of NLS and show that they reduce to the above three classical homoclinic solutions for particular spectral values in the periodic inverse scattering transform. Furthermore, I discuss another infinity of solutions to the NLS equation that are not classifiable as homoclinic solutions. These latter are the genus-2N theta function solutions of the NLS equation: they are the most general unstable spectral solutions for periodic boundary conditions. I further describe how the homoclinic solutions of the NLS equation, for N = 1, can be derived directly from the theta functions in a particular limit. The solutions I address herein are actual spectral components in the nonlinear Fourier transform theory for the NLS equation: The periodic inverse scattering transform. The main purpose of this paper is to discuss a broader class of rogue wave packets1 for ship design, as defined in the Extreme Seas program. The spirit of this research came from D. Faulkner (2000) who many years ago suggested that ship design procedures, in order to take rogue waves into account, should progress beyond the use of simple sine waves. 1An overview of other work in the field of rogue waves is given elsewhere: Osborne 2010, 2012 and 2013. See the books by Olagnon and colleagues 2000, 2004 and 2008 for the Brest meetings. The books by Kharif et al. (2008) and Pelinovsky et al. (2010) are excellent references.

Mechanism for G2 phase-specific nuclear export of the kinetochore protein CENP-F.

PubMed

Loftus, Kyle M; Cui, Heying; Coutavas, Elias; King, David S; Ceravolo, Amanda; Pereiras, Dylan; Solmaz, Sozanne R

2017-08-03

Centromere protein F (CENP-F) is a component of the kinetochore and a regulator of cell cycle progression. CENP-F recruits the dynein transport machinery and orchestrates several cell cycle-specific transport events, including transport of the nucleus, mitochondria and chromosomes. A key regulatory step for several of these functions is likely the G2 phase-specific export of CENP-F from the nucleus to the cytosol, where the cytoplasmic dynein transport machinery resides; however, the molecular mechanism of this process is elusive. Here, we have identified 3 phosphorylation sites within the bipartite classical nuclear localization signal (cNLS) of CENP-F. These sites are specific for cyclin-dependent kinase 1 (Cdk1), which is active in G2 phase. Phosphomimetic mutations of these residues strongly diminish the interaction of the CENP-F cNLS with its nuclear transport receptor karyopherin α. These mutations also diminish nuclear localization of the CENP-F cNLS in cells. Notably, the cNLS is phosphorylated in the -1 position, which is important to orient the adjacent major motif for binding into its pocket on karyopherin α. We propose that localization of CENP-F is regulated by a cNLS, and a nuclear export pathway, resulting in nuclear localization during most of interphase. In G2 phase, the cNLS is weakened by phosphorylation through Cdk1, likely resulting in nuclear export of CENP-F via the still active nuclear export pathway. Once CENP-F resides in the cytosol, it can engage in pathways that are important for cell cycle progression, kinetochore assembly and the faithful segregation of chromosomes into daughter cells.

University education for the physically disabled

PubMed Central

Gunn, Alexander D. G.

1977-01-01

Two per thousand of the student population are physically handicapped. The facilities for the care of physically disabled students were surveyed at the 34 universities that have full-time medical officers and primary medical teams. It was found that communication before arrival about special medical and nursing needs of the student is insufficient, that there is inadequate liaison within the universities between the admissions office and the medical unit, and a lack of published statements by the universities about the admission of the physically disabled. In contrast, the facilities available for the handicapped student are considerable, the academic success rate high, the range of serious disability being successfully coped with is extensive, and there is a need for more encouragement in the community for the physically disabled to undertake, where intellectually capable, a university education. ImagesFigure 1.Figure 2.Figure 3. PMID:140240

Structural analysis of the complex between influenza B nucleoprotein and human importin-α.

PubMed

Labaronne, Alice; Milles, Sigrid; Donchet, Amélie; Jensen, Malene Ringkjøbing; Blackledge, Martin; Bourhis, Jean-Marie; Ruigrok, Rob W H; Crépin, Thibaut

2017-12-07

Influenza viruses are negative strand RNA viruses that replicate in the nucleus of the cell. The viral nucleoprotein (NP) is the major component of the viral ribonucleoprotein. In this paper we show that the NP of influenza B has a long N-terminal tail of 70 residues with intrinsic flexibility. This tail contains the Nuclear Location Signal (NLS). The nuclear trafficking of the viral components mobilizes cellular import factors at different stages, making these host-pathogen interactions promising targets for new therapeutics. NP is imported into the nucleus by the importin-α/β pathway, through a direct interaction with importin-α isoforms. Here we provide a combined nuclear magnetic resonance and small-angle X-ray scattering (NMR/SAXS) analysis to describe the dynamics of the interaction between influenza B NP and the human importin-α. The NP of influenza B does not have a single NLS nor a bipartite NLS but our results suggest that the tail harbors several adjacent NLS sequences, located between residues 30 and 71.

Characterization of a nuclear localization signal in the C-terminus of the adeno-associated virus Rep68/78 proteins

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cassell, Geoffrey D.; Weitzman, Matthew D.

2004-10-01

Adeno-associated virus (AAV) replicates in the nucleus of infected cells, and therefore multiple nuclear import events are required for productive infection. We analyzed nuclear import of the viral Rep proteins and characterized a nuclear localization signal (NLS) in the C-terminus. We demonstrate that basic residues in this region constitute an NLS that is transferable and mediates interaction with the nuclear import receptor importin {alpha} in vitro. Mutant Rep proteins are predominantly cytoplasmic and are severely compromised for interactions with importin {alpha}, but retain their enzymatic functions in vitro. Interestingly, mutations of the NLS had significantly less effect on importin {alpha}more » interaction and replication in the context of Rep78 than when incorporated into the Rep68 protein. Together, our results demonstrate that a bipartite NLS exists in the shared part of Rep68 and Rep78, and suggest that an alternate entry mechanism may also contribute to nuclear localization of the Rep78 protein.« less

38 CFR 18.447 - Nonacademic services.

Code of Federal Regulations, 2010 CFR

2010-07-01

....447 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education... discriminate on the basis of handicap. A recipient that offers physical education courses or that operates or... students physical education and athletic activities that are separate or different only if separation or...

¹¹¹In-Bn-DTPA-nimotuzumab with/without modification with nuclear translocation sequence (NLS) peptides: an Auger electron-emitting radioimmunotherapeutic agent for EGFR-positive and trastuzumab (Herceptin)-resistant breast cancer.

PubMed

Fasih, Aisha; Fonge, Humphrey; Cai, Zhongli; Leyton, Jeffrey V; Tikhomirov, Ilia; Done, Susan J; Reilly, Raymond M

2012-08-01

Increased expression of epidermal growth factor receptors (EGFR) in breast cancer (BC) is often associated with trastuzumab (Herceptin)-resistant forms of the disease and represents an attractive target for novel therapies. Nimotuzumab is a humanized IgG(1) monoclonal antibody that is in clinical trials for treatment of EGFR-overexpressing malignancies. We show here that nimotuzumab derivatized with benzylisothiocyanate diethylenetriaminepentaacetic acid for labelling with the subcellular range Auger electron-emitter, (111)In and modified with nuclear translocation sequence (NLS) peptides ((111)In-NLS-Bn-DTPA-nimotuzumab) was bound, internalized and transported to the nucleus of EGFR-positive BC cells. Emission of Auger electrons in close proximity to the nucleus caused multiple DNA double-strand breaks which diminished the clonogenic survival (CS) of MDA-MB-468 cells that have high EGFR density (2.4 × 10(6) receptors/cell) to less than 3 %. (111)In-Bn-DTPA-nimotuzumab without NLS peptide modification was sevenfold less effective for killing MDA-MB-468 cells. (111)In-Bn-DTPA-nimotuzumab with/without NLS peptide modification were equivalently cytotoxic to MDA-MB-231 and TrR1 BC cells that have moderate EGFR density (5.4 × 10(5) or 4.2 × 10(5) receptors/cell, respectively) reducing their CS by twofold. MDA-MB-231 cells have intrinsic trastuzumab resistance due to low HER2 density, whereas TrR1 cells have acquired resistance despite HER2 overexpression. Biodistribution and microSPECT/CT imaging revealed that (111)In-NLS-Bn-DTPA-nimotuzumab exhibited more rapid elimination from the blood and lower tumour uptake than (111)In-Bn-DTPA-nimotuzumab. Tumour uptake of the radioimmunoconjugates in mice with MDA-MB-468 xenografts was high (8-16 % injected dose/g) and was blocked by administration of an excess of unlabelled nimotuzumab, demonstrating EGFR specificity. We conclude that (111)In-Bn-DTPA-nimotuzumab with/without NLS peptide modification are promising Auger electron-emitting radioimmunotherapeutic agents for EGFR-positive BC, but (111)In-Bn-DTPA-nimotuzumab may be preferred due to its higher tumour uptake in vivo.

The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme

PubMed Central

Wang, Yi-Ping; Du, Wen-Juan; Huang, Li-Ping; Wei, Yan-Wu; Wu, Hong-Li; Feng, Li; Liu, Chang-Ming

2016-01-01

Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme. Its nuclear localization is a prerequisite for its enzymatic function in the initiation of viral DNA replication. However, the mechanisms by which the PRV DNA polymerase holoenzyme enters the nucleus have not been determined. In this study, we characterized the nuclear import pathways of the PRV DNA polymerase catalytic and accessory subunits. Immunofluorescence analysis showed that UL42 localizes independently in the nucleus, whereas UL30 alone predominantly localizes in the cytoplasm. Intriguingly, the localization of UL30 was completely shifted to the nucleus when it was coexpressed with UL42, demonstrating that nuclear transport of UL30 occurs in an UL42-dependent manner. Deletion analysis and site-directed mutagenesis of the two proteins showed that UL42 contains a functional and transferable bipartite nuclear localization signal (NLS) at amino acids 354–370 and that K354, R355, and K367 are important for the NLS function, whereas UL30 has no NLS. Coimmunoprecipitation assays verified that UL42 interacts with importins α3 and α4 through its NLS. In vitro nuclear import assays demonstrated that nuclear accumulation of UL42 is a temperature- and energy-dependent process and requires both importins α and β, confirming that UL42 utilizes the importin α/β-mediated pathway for nuclear entry. In an UL42 NLS-null mutant, the UL42/UL30 heterodimer was completely confined to the cytoplasm when UL42 was coexpressed with UL30, indicating that UL30 utilizes the NLS function of UL42 for its translocation into the nucleus. Collectively, these findings suggest that UL42 contains an importin α/β-mediated bipartite NLS that transports the viral DNA polymerase holoenzyme into the nucleus in an in vitro expression system. PMID:26913023

Nonlinear Ocean Waves

DTIC Science & Technology

1994-01-06

for all of this work is the fact that the Kadomtsev - Petviashvili equation , a1(atu + ui)xU + a.3u) + ay2u = 0, (KP) describes approximately the evolution...the contents of these two papers. (a) Numerically induced chaos The cubic-nonlinear Schrtdinger equation in one dimension, iatA +,2V + 21i,1 =0, (NLS...arises in several physical contexts, including the evolution of nearly monochromatic, one-dimensional waves in deep water. The equation is known to be

Radiating dispersive shock waves in non-local optical media

PubMed Central

El, Gennady A.

2016-01-01

We consider the step Riemann problem for the system of equations describing the propagation of a coherent light beam in nematic liquid crystals, which is a general system describing nonlinear wave propagation in a number of different physical applications. While the equation governing the light beam is of defocusing nonlinear Schrödinger (NLS) equation type, the dispersive shock wave (DSW) generated from this initial condition has major differences from the standard DSW solution of the defocusing NLS equation. In particular, it is found that the DSW has positive polarity and generates resonant radiation which propagates ahead of it. Remarkably, the velocity of the lead soliton of the DSW is determined by the classical shock velocity. The solution for the radiative wavetrain is obtained using the Wentzel–Kramers–Brillouin approximation. It is shown that for sufficiently small initial jumps the nematic DSW is asymptotically governed by a Korteweg–de Vries equation with the fifth-order dispersion, which explicitly shows the resonance generating the radiation ahead of the DSW. The constructed asymptotic theory is shown to be in good agreement with the results of direct numerical simulations. PMID:27118911

Integrable discretisations for a class of nonlinear Schrödinger equations on Grassmann algebras

NASA Astrophysics Data System (ADS)

Grahovski, Georgi G.; Mikhailov, Alexander V.

2013-12-01

Integrable discretisations for a class of coupled (super) nonlinear Schrödinger (NLS) type of equations are presented. The class corresponds to a Lax operator with entries in a Grassmann algebra. Elementary Darboux transformations are constructed. As a result, Grassmann generalisations of the Toda lattice and the NLS dressing chain are obtained. The compatibility (Bianchi commutativity) of these Darboux transformations leads to integrable Grassmann generalisations of the difference Toda and NLS equations. The resulting systems will have discrete Lax representations provided by the set of two consistent elementary Darboux transformations. For the two discrete systems obtained, initial value and initial-boundary problems are formulated.

Cycle 0(CY1991) NLS trade studies and analyses report. Book 1: Structures and core vehicle

NASA Technical Reports Server (NTRS)

1992-01-01

This report (SR-1: Structures, Trades, and Analysis), documents the Core Tankage Trades and analyses performed in support of the National Launch System (NLS) Cycle 0 preliminary design activities. The report covers trades that were conducted on the Vehicle Assembly, Fwd Skirt, LO2 Tank, Intertank, LH2 Tank, and Aft Skirt of the NLS Core Tankage. For each trade study, a two page executive summary and the detail trade study are provided. The trade studies contain study results, recommended changes to the Cycle 0 Baselines, and suggested follow on tasks to be performed during Cycle 1.

Living with a Handicap. Australian Early Childhood Resource Booklets, No. 3, 1981.

ERIC Educational Resources Information Center

Australian Early Childhood Association, Inc., Watson.

Autobiographical sketches of four Australians living with physical handicaps are presented in this Australian early childhood resource booklet. Life experiences in school and out are reported by a blind man, a young woman who was brain damaged due to injuries in an automobile accident, a mother of a child with spina bifida, and a Doctor of…

Physical Education for Poorly Co-ordinated Children. The Promotion of Body Awareness and the Development of Basic Skills.

ERIC Educational Resources Information Center

Watson, Bevilie R.

This book deals with the promotion of body awareness through use of educational gymnastics, with handicapped or non-handicapped but poorly coordinated children of all age levels. The method provides an analytical approach to movement, encourages children to listen to language, and assists them in creating their own activities. The activities and…

Libraries for People with Handicaps: A Directory of Public Library Resources and Services in Ohio. Second edition.

ERIC Educational Resources Information Center

Ohio State Library, Columbus.

This second edition of the directory contains information collected from 249 public libraries for use by the handicapped, i.e., blind, physically disabled, aged, shut-in, and institutionalized persons. Several changes in format have been made in response to users' reactions to the first edition. For quick reference to library services, materials…

Barrier free access; right or privilege.

PubMed

Collings, G D

1976-09-01

Physically handicapped individuals and the elderly have for too long been denied access to public facilities and transportation systems. Barrier free access is a fundamental guarantee necessary for all handicapped and elderly if they are to become and remain contributing members of our society. A review is provided of legislation, constitutional theories, and current litigation within the context of providing a barrier free environment.

Happiness Is Healthiness. A Special Study Institute in Health Education for Mentally Handicapped Children in Elementary and Secondary Schools.

ERIC Educational Resources Information Center

New York State Education Dept., Albany. Bureau for Mentally Handicapped Children.

Presented are proceedings from a 3-day study institute (1971) to provide teachers of mentally handicapped (MH) students, health profession personnel, and physical education teachers in Schenectady with resource information for implementation of New York state's mandate to provide health education for all children. Included are the program schedule…

Report and Evaluation of the Second Annual Workshop for Vocational Education Personnel Working with the Handicapped.

ERIC Educational Resources Information Center

Sheppard, N. Alan

The report describes an inservice workshop for vocational education teachers of the physically, mentally, and multiply handicapped. The first third of the report consists of a general introduction and a summary of workshop activities with respect to (1) general information, (2) topics covered, (3) requirements for a letter grade, (4) summaries of…

Proposal for a Library Project for Severely-Profoundly Retarded, Multiple Handicapped Individuals.

ERIC Educational Resources Information Center

South Dakota State Library, Pierre.

A proposal for a library project for mentally and physically handicapped persons at Custer State Hospital includes a listening library for the residents of the hospital with a cassette player in each resident's room. Tape storage would be at the nurse's station on each floor, and the charge nurse on that floor would be responsible for the tapes.…

Pre-Placement Program for Severely Multi-Handicapped Deaf-Blind Children, 1980-1981. Final Evaluation Report.

ERIC Educational Resources Information Center

Tobias, Robert; And Others

Evaluation of the sixth and final year of operation for a preplacement program for 13 severely multiply handicapped deaf blind children, located in the Industrial Home for the Blind, is reported. The program is explained to prepare students for entrance into their existing special education programs. Qualitative findings on the physical setting,…

Study of Man-Machine Communications Systems for Disabled Persons (The Handicapped). Volume VI. Final Report.

ERIC Educational Resources Information Center

Kafafian, Haig

The instruction manual contains lessons for teaching severely physically and/or neurologically handicapped students to use the seven-key Cybertype electric writing machine. Unlike the 14-key keyboard, which requires bilateral coordination in arms, legs, or other parts of the body, the seven-key keyboard requires the use of only one part of the…

Projets Pilotes Quebecois Portant sur L'Integration Scolaire aux Niveaux Pre-Scolaire et Primaire (Quebec Pilot Projects on Mainstreaming of Preschool and Primary-Level Schoolchildren).

ERIC Educational Resources Information Center

Saint-Laurent, Lise; And Others

1992-01-01

This article synthesizes 55 Quebec (Canada) pilot projects on mainstreaming of pupils with learning disabilities, behavior disorders, physical or sensorial handicaps, or mental handicaps, at preschool and elementary levels. It focuses on extent of integration, interventions, training and support for teachers, and evaluation methodology used.…

Zinc oxide nanoleaves: A scalable disperser-assisted sonochemical approach for synthesis and an antibacterial application.

PubMed

Gupta, Anadi; Srivastava, Rohit

2018-03-01

Current study reports a new and highly scalable method for the synthesis of novel structure Zinc oxide nanoleaves (ZnO-NLs) using disperser-assisted sonochemical approach. The synthesis was carried out in different batches from 50mL to 1L to ensure the scalability of the method which produced almost similar results. The use of high speed (9000rpm) mechanical dispersion while bath sonication (200W, 33kHz) yield 4.4g of ZnO-NLs powder in 1L batch reaction within 2h (>96% yield). The ZnO-NLs shows an excellent thermal stability even at a higher temperature (900°C) and high surface area. The high antibacterial activity of ZnO-NLs against diseases causing Gram-positive bacteria Staphylococcus aureus shows a reduction in CFU, morphological changes like eight times reduction in cell size, cell burst, and cellular leakage at 200µg/mL concentration. This study provides an efficient, cost-effective and an environmental friendly approach for the synthesis of ZnO-NLs at industrial scale as well as new technique to increase the efficiency of the existing sonochemical method. We envisage that this method can be applied to various fields where ZnO is significantly consumed like rubber manufacturing, ceramic industry and medicine. Copyright © 2017 Elsevier B.V. All rights reserved.

Kinetic analysis of hyperpolarized data with minimum a priori knowledge: Hybrid maximum entropy and nonlinear least squares method (MEM/NLS).

PubMed

Mariotti, Erika; Veronese, Mattia; Dunn, Joel T; Southworth, Richard; Eykyn, Thomas R

2015-06-01

To assess the feasibility of using a hybrid Maximum-Entropy/Nonlinear Least Squares (MEM/NLS) method for analyzing the kinetics of hyperpolarized dynamic data with minimum a priori knowledge. A continuous distribution of rates obtained through the Laplace inversion of the data is used as a constraint on the NLS fitting to derive a discrete spectrum of rates. Performance of the MEM/NLS algorithm was assessed through Monte Carlo simulations and validated by fitting the longitudinal relaxation time curves of hyperpolarized [1-(13) C] pyruvate acquired at 9.4 Tesla and at three different flip angles. The method was further used to assess the kinetics of hyperpolarized pyruvate-lactate exchange acquired in vitro in whole blood and to re-analyze the previously published in vitro reaction of hyperpolarized (15) N choline with choline kinase. The MEM/NLS method was found to be adequate for the kinetic characterization of hyperpolarized in vitro time-series. Additional insights were obtained from experimental data in blood as well as from previously published (15) N choline experimental data. The proposed method informs on the compartmental model that best approximate the biological system observed using hyperpolarized (13) C MR especially when the metabolic pathway assessed is complex or a new hyperpolarized probe is used. © 2014 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc.

Experience of handicap and anxiety in phobic postural vertigo.

PubMed

Holmberg, Johan; Karlberg, Mikael; Harlacher, Uwe; Magnusson, Mans

2005-03-01

We found a difference in gender distribution in a population of phobic postural vertigo patients compared with dizzy patients seen in general neuro-otological practice. It appears as if women with phobic postural vertigo suffer more and are more handicapped by dizziness than both men with phobic postural vertigo and a population with dizziness. These differences may reflect other causes of phobic postural vertigo besides anxiety, such as gender-related coping behaviour and postural strategy. Anxiety influences the degree of suffering and handicap in dizzy patients. Experiences of anxiety and handicap were investigated among a population with phobic postural vertigo. Using the Dizziness Handicap Inventory, the Vertigo Symptom Scale and the Vertigo Handicap Questionnaire, 34 consecutive patients with phobic postural vertigo were compared with a population of 95 consecutive patients seen at a balance disorder clinic. Patients with phobic postural vertigo scored higher than the control subjects with respect to all parameters with the exception of the physical subscale of the Dizziness Handicap Inventory. Because there were significantly more women in the control group we performed a gender-specific analysis of the results. The higher test scores among patients with phobic postural vertigo can be explained by the higher scores among women in this group, while the test results for men were more similar to those of the control group.

Relationship between impairments, disability and handicap in reflex sympathetic dystrophy patients: a long-term follow-up study.

PubMed

Geertzen, J H; Dijkstra, P U; van Sonderen, E L; Groothoff, J W; ten Duis, H J; Eisma, W H

1998-10-01

To determine the relationship between impairments, disability and handicap in reflex sympathetic dystrophy (RSD) patients. A long-term follow-up study of upper extremity RSD patients. A university hospital. Sixty-five patients, 3-9 years (mean interval 5.5 years) after RSD of the upper extremity (mean age 50.2 years). Impairments: range of motion, moving two point discrimination, muscle strength of the hand and pain were measured. Disability was assessed with the Groningen Activity Restriction Scale (GARS) and handicap was assessed with three subscales (social functioning, role limitations due to physical problems and role limitations due to emotional problems) of the RAND-36. After RSD of the upper extremity, 62% of the patients are limited in activities of daily living (ADL) and/or instrumental ADL (IADL). Pain and restrictions in forward flexion of the shoulder, thumb opposition and grip strength are the most important impairments limiting ADL and IADL. Patients with limitations in ADL and IADL are significantly more handicapped than patients without limitations. Pain is the most important factor contributing to handicap. The relationship between impairments and disability and between disability and handicap in RSD patients is weak to moderate. Pain is the most important factor leading to disability and handicap.

Growth at adolescence. Clinical correlates.

PubMed

Daniel, W A

1985-03-01

Several highly significant changes occur within a relatively short period of time during adolescence. Great alteration in physique, developmental progress in thinking, and psychologic gains toward attaining ego identity take place but not always synchronously. Attention is paid to physical changes because they are visible and are of intense concern to adolescents, but physicians and other professionals should remember cognitive and psychosocial growth are affected by physical growth, and vice versa. Often there is a temporary disequilibrium in the relationship of these three areas of growth, and this can affect one or another part of the developmental pattern. It is therefore necessary to remind ourselves of the diversity of adolescent growth, and of adolescents, when caring for a young patient and be cognizant of growth in areas other than physical. More and more children with congenital or acquired handicaps are living to become adolescents and perhaps adults. Handicaps can be limited to one of the three major areas of growth or involve them all in varying degrees. For example, sickle cell disease, Crohn's disease, or ulcerative colitis may postpone physical growth for a significant period; this lack of pubertal change can affect psychosocial development but usually does not impair cognitive growth. Mental retardation may have no apparent effect on physical growth but can handicap the adolescent's psychosocial development. Growth still occurs in a sequential pattern but often it seems that handicapped youngsters reach a developmental milestone by a series of "detours." Physicians must recognize these lags or differences and try to facilitate progress, promote self-esteem, and provide understanding. Much can be done with anticipatory guidance. Adolescence often provides the opportunity to overcome past damage or, in some instances, to start anew on a more optimal program for physical and psychosocial growth. Young adolescent boys and girls usually look to the physician for factual information and guidance; they long for understanding by an adult outside of the family. If we can successfully fill their expectations, adolescents will be the better for it.

Developing job-related preplacement medical examinations.

PubMed

Hogan, J C; Bernacki, E J

1981-07-01

Federal regulations prohibiting discrimination in hiring require that employment selection procedures to evaluate applicants be based on job-related criteria. The preplacement physical examination used in employment, particularly in the placement of handicapped persons, must also be conducted in a job-related manner. This paper discusses the development and use of the physical examination in selecting and placing applicants for jobs in the workplace with special reference to handicapped persons and disabled veterans. It presents and justifies a method of performing these examinations in a manner consistent with humanistic and business goals as well as the goals of federal regulatory agencies prohibiting employment discrimination.

46 CFR 153.483 - Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1, 1986...

Code of Federal Regulations, 2010 CFR

2010-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.483 Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1...

46 CFR 153.480 - Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986: Categories...

Code of Federal Regulations, 2012 CFR

2012-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.480 Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986...

46 CFR 153.480 - Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986: Categories...

Code of Federal Regulations, 2013 CFR

2013-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.480 Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986...

46 CFR 153.480 - Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986: Categories...

Code of Federal Regulations, 2014 CFR

2014-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.480 Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986...

46 CFR 153.483 - Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1, 1986...

Code of Federal Regulations, 2014 CFR

2014-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.483 Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1...

46 CFR 153.480 - Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986: Categories...

Code of Federal Regulations, 2010 CFR

2010-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.480 Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986...

46 CFR 153.480 - Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986: Categories...

Code of Federal Regulations, 2011 CFR

2011-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.480 Stripping quantity for Category B and C NLS tanks on ships built after June 30, 1986...

46 CFR 153.483 - Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1, 1986...

Code of Federal Regulations, 2011 CFR

2011-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.483 Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1...

46 CFR 153.483 - Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1, 1986...

Code of Federal Regulations, 2012 CFR

2012-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.483 Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1...

46 CFR 153.483 - Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1, 1986...

Code of Federal Regulations, 2013 CFR

2013-10-01

..., LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Design and Equipment for Pollution Control § 153.483 Restricted voyage waiver for Category B and C NLS tanks on ships built before July 1...

Instructional Aids for Adaptive Physical Education.

ERIC Educational Resources Information Center

Cowart, James F.

This idea book on remedial physical education is divided into four sections. Each section contains drawings and brief explanations on ways to adapt physical education for the physically handicapped. The first section covers homemade equipment for developing muscular strength and endurance in specific muscles. Also included are resistive exercises…

7 CFR 15b.35 - Nonacademic services.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid... discriminate on the basis of handicap. A recipient that offers physical education courses or that operates or... students physical education and athletic activities that are separate or different only if separation or...

[Cross-cultural adaptation and validation of the Dizziness Handicap Inventory: Argentine version].

PubMed

Caldara, Betina; Asenzo, Adriana I; Brusotti Paglia, Gabriela; Ferreri, Eliana; Gomez, Ramiro S; Laiz, Mariela M; Luques, María L; Mangoni, Ana P; Marazzi, Carla; Matesa, María A; Peker, Guillermo; Pratto, Romina A; Quiroga, Cecilia E; Rapela, Laura; Ruiz, Vanesa R; Sanchez, Noelia; Taglioretti, Célide L; Tana, Andrés M; Zandstra, Ingrid V

2012-01-01

The Dizziness Handicap Inventory is a useful tool for quantifying self-perceived handicap in patients with vertigo, dizziness or unsteadiness and its impact on daily living activities. The Dizziness Handicap Inventory identifies functional, physical and emotional disorders related to balance disturbance. Our objective was to cross-culturally adapt the Peninsular Spanish version of the Dizziness Handicap Inventory for use in Argentina and validate the adapted Argentinian version. We included both healthy subjects and patients with vertigo, dizziness or unsteadiness, aged 18 to 85 years, native Spanish-speaking Argentinians. We introduced linguistic and cultural modifications to the Peninsular Spanish version to obtain the Argentinian one. This version was given twice to 108 patients, 24 to 72 h apart. Internal consistency, test-retest reliability and construct validity were assessed using a visual analogue scale, the Romberg test, the tandem Romberg test and the tandem gait test. We found high internal consistency (α=0.87) and very high test-retest reliability for the total Dizziness Handicap Inventory score (intraclass correlation coefficient: 0.98) and its subscales. The total Dizziness Handicap Inventory and the functional subscale were found to correlate significantly with the Romberg and tandem Romberg tests. The emotional subscale showed a significant correlation with the Romberg test and the eyes-open tandem Romberg test (P<.05) The Argentinian version of the Dizziness Handicap Inventory proved to be a reliable and valid tool to quantify self-perceived handicap resulting from vertigo, dizziness or unsteadiness. Copyright © 2011 Elsevier España, S.L. All rights reserved.

Preparation of physical therapists to work with handicapped infants and their families: current status and training needs.

PubMed

Cochrane, C G; Farley, B G; Wilhelm, I J

1990-06-01

The purposes of this study were to determine the current status of physical therapists' preparation to work with handicapped and at-risk infants and their families and to identify needs for infant- and family-focused training materials and curricula. Results of a telephone survey of 73 physical therapy programs and a follow-up mail survey of 14 physical therapy programs with infancy specialization options are presented. Students in entry-level programs and postprofessional master's degree programs with infancy specializations commonly received instruction in infancy-related topics. Many students received minimal or no exposure to family-related content. Family assessment and intervention were identified as the areas of highest priority for development of training materials and curricula. The results of this study provide direction for the design of infant- and family-focused training materials and curricula in physical therapy.

The White House Conference on Handicapped Individuals: Volume II. Final Report. Part A. May 23-27, 1977.

ERIC Educational Resources Information Center

1977

The three-part final report documents recommendations and resolutions and summarizes the work of more than 700 delegates to the White House Conference on Handicapped Individuals, May 23-27, 1977, in Washington, D.C. It is emphasized that the primary purpose of the conference was to give persons with mental and/or physical disabilities an…

The July 2010 Outburst of the NLS1 PMN J0948+0022

NASA Technical Reports Server (NTRS)

Foschini, L.; Ghisellini, G.; Marashi, L.; Tagliaferri, G.; Tavecchio, F.; Kovalev, Y. Y.; Kovalev, Yu. A.; Lister, M. L.; Richards, J. L.; D'Ammando, F.;

The Hallermann-Streiff Syndrome

ERIC Educational Resources Information Center

Nevin, N. C.; And Others

1974-01-01

Described are the physical and genetic factors in a case of Hallermann-Streiff Syndrome (characterized by dwarfism and other anomalies, with or without retardation) in an 11-year old severely physically and mentally handicapped Irish girl. (CL)

The self-serving function of hypochondriacal complaints: physical symptoms as self-handicapping strategies.

PubMed

Smith, T W; Snyder, C R; Perkins, S C

1983-04-01

The present experiment tested the hypothesis that hypochondriacal individuals commonly use reports of physical illness and symptoms as a strategy to control attributions made about their performances in evaluative settings (i.e., self-handicapping strategies). Specifically, it was predicted that hypochondriacal individuals would report more recent physical illness and complaints and more current physical symptoms in an evaluative setting in which poor health could serve as an alternative explanation for poor performance than would either individuals in an evaluative setting in which poor health was precluded as an excuse or individuals in a nonevaluative setting. As predicted, results supported this self-protective pattern of complaints in a hypochondriacal sample but not in a nonhypochondriacal group. The self-protective role of hypochondriacal behavior is discussed in relation to other theory and research on the nature and treatment of hypochondriasis.

Physical Requirements of the Workplace: Research Considerations for Personnel Selection.

ERIC Educational Resources Information Center

Hogan, Joyce C.

Personnel selection processes for physically demanding jobs often discriminate against women, minorities, and handicapped individuals. Developing and validating job-related performance tests requires the assessment of the physical demands of a job and of the same physical capabilities in individuals. Job analysis is a systematic assessment…

Guide for Teaching Physical Education, Grades 7-12.

ERIC Educational Resources Information Center

South Carolina State Dept. of Education, Columbia.

GRADES OR AGES: Grades 7-12. SUBJECT MATTER: Physical education, including team sports, stunts, tumbling and gymnastics, track and field, swimming, individual and dual sports, physical education for the handicapped, outdoor education, and rhythmic activities. ORGANIZATION AND PHYSICAL APPEARANCE: The guide is divided into 16 chapters, each of…

45 CFR 1170.47 - Nonacademic services.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ASSISTED PROGRAMS OR ACTIVITIES Postsecondary Education § 1170.47 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits... the basis of handicap. A recipient that offers physical education courses or that operates or sponsors...

Summative Evaluation of the National Literacy Secretariat. Final Report

ERIC Educational Resources Information Center

Human Resources and Skills Development Canada, 2004

2004-01-01

The National Literacy Secretariat (NLS) is a grants and contributions initiative established in 1987. The NLS has two objectives: (1) To increase opportunities and take-up, so that people improve their reading and writing skills; and (2) To work towards making Canada's social, economic and political life more accessible to those with weak literacy…

33 CFR 158.310 - Reception facilities: General.

Code of Federal Regulations, 2010 CFR

2010-07-01

... order to pass the inspection under § 158.160, must— (1) Be a reception facility as defined under § 158... residue; (5) Be capable of receiving NLS residue from an oceangoing ship within 24 hours after notice by that ship of the need for reception facilities; and (6) Be capable of completing the transfer of NLS...

33 CFR 151.33 - Certificates needed to carry Category C Oil-like NLS.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships Noxious Liquid Substance Pollution § 151.33 Certificates needed to carry Category C Oil-like NLS. (a) A U.S. oceangoing ship may not... HOMELAND SECURITY (CONTINUED) POLLUTION VESSELS CARRYING OIL, NOXIOUS LIQUID SUBSTANCES, GARBAGE, MUNICIPAL...

46 CFR 153.2 - Definitions and acronyms.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., including hydrofoils, air-cushion vehicles, submersibles, floating craft whether self-propelled or not, and fixed or floating platforms. Slop tanks include slop tanks and cargo tanks used as slop tanks. Solidifying NLS means a Category A, B, or C NLS that has a melting point— (1) Greater than 0 °C but less than...

33 CFR 151.35 - Certificates needed to carry Category D NLS and Category D Oil-like NLS.

Code of Federal Regulations, 2014 CFR

2014-07-01

... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION VESSELS CARRYING OIL, NOXIOUS LIQUID SUBSTANCES, GARBAGE, MUNICIPAL OR COMMERCIAL WASTE, AND BALLAST WATER Implementation of MARPOL 73/78 and the Protocol on Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships Noxious...

33 CFR 151.35 - Certificates needed to carry Category D NLS and Category D Oil-like NLS.

Code of Federal Regulations, 2012 CFR

2012-07-01

... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION VESSELS CARRYING OIL, NOXIOUS LIQUID SUBSTANCES, GARBAGE, MUNICIPAL OR COMMERCIAL WASTE, AND BALLAST WATER Implementation of MARPOL 73/78 and the Protocol on Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships Noxious...

33 CFR 151.35 - Certificates needed to carry Category D NLS and Category D Oil-like NLS.

Code of Federal Regulations, 2011 CFR

2011-07-01

... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION VESSELS CARRYING OIL, NOXIOUS LIQUID SUBSTANCES, GARBAGE, MUNICIPAL OR COMMERCIAL WASTE, AND BALLAST WATER Implementation of MARPOL 73/78 and the Protocol on Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships Noxious...

33 CFR 151.35 - Certificates needed to carry Category D NLS and Category D Oil-like NLS.

Code of Federal Regulations, 2010 CFR

2010-07-01

... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION VESSELS CARRYING OIL, NOXIOUS LIQUID SUBSTANCES, GARBAGE, MUNICIPAL OR COMMERCIAL WASTE, AND BALLAST WATER Implementation of MARPOL 73/78 and the Protocol on Environmental Protection to the Antarctic Treaty as it Pertains to Pollution from Ships Noxious...

Solitary wave for a nonintegrable discrete nonlinear Schrödinger equation in nonlinear optical waveguide arrays

NASA Astrophysics Data System (ADS)

Ma, Li-Yuan; Ji, Jia-Liang; Xu, Zong-Wei; Zhu, Zuo-Nong

2018-03-01

We study a nonintegrable discrete nonlinear Schrödinger (dNLS) equation with the term of nonlinear nearest-neighbor interaction occurred in nonlinear optical waveguide arrays. By using discrete Fourier transformation, we obtain numerical approximations of stationary and travelling solitary wave solutions of the nonintegrable dNLS equation. The analysis of stability of stationary solitary waves is performed. It is shown that the nonlinear nearest-neighbor interaction term has great influence on the form of solitary wave. The shape of solitary wave is important in the electric field propagating. If we neglect the nonlinear nearest-neighbor interaction term, much important information in the electric field propagating may be missed. Our numerical simulation also demonstrates the difference of chaos phenomenon between the nonintegrable dNLS equation with nonlinear nearest-neighbor interaction and another nonintegrable dNLS equation without the term. Project supported by the National Natural Science Foundation of China (Grant Nos. 11671255 and 11701510), the Ministry of Economy and Competitiveness of Spain (Grant No. MTM2016-80276-P (AEI/FEDER, EU)), and the China Postdoctoral Science Foundation (Grant No. 2017M621964).

Transduction of NeuroD2 protein induced neural cell differentiation.

PubMed

Noda, Tomohide; Kawamura, Ryuzo; Funabashi, Hisakage; Mie, Masayasu; Kobatake, Eiry

2006-11-01

NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta100-115) (lack of NLS), ND2(Delta123-134) (lack of basic) and ND2(Delta100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein.

The radio structure of the peculiar narrow-line Seyfert 1 galaxy candidate J1100+4421

NASA Astrophysics Data System (ADS)

Gabányi, K. É.; Frey, S.; Paragi, Z.; Järvelä, E.; Morokuma, T.; An, T.; Tanaka, M.; Tar, I.

2018-01-01

Narrow-line Seyfert 1 galaxies (NLS1) are an intriguing subclass of active galactic nuclei. Their observed properties indicate low central black hole mass and high accretion rate. The extremely radio-loud NLS1 sources often show relativistic beaming and are usually regarded as younger counterparts of blazars. Recently, the object SDSS J110006.07+442144.3 was reported as a candidate NLS1 source. The characteristics of its dramatic optical flare indicated its jet-related origin. The spectral energy distribution of the object was similar to that of the γ-ray detected radio-loud NLS1, PMN J0948+0022. Our high-resolution European very long baseline interferometry network observations at 1.7 and 5 GHz revealed a compact core feature with a brightness temperature of ≳1010 K. Using the lowest brightness temperature value and assuming a moderate Lorentz factor of ∼9, the jet viewing angle is ≲26°. Archival Very Large Array data show a large-scale radio structure with a projected linear size of ∼150 kpc reminiscent of double-sided morphology.

Disability in Activities of Daily Living and Severity of Dyskinesias Determine the Handicap of Parkinson's Disease Patients in Advanced Stage Selected to DBS.

PubMed

Coelho, Miguel; Abreu, Daisy; Correia-Guedes, Leonor; Lobo, Patricia Pita; Fabbri, Margherita; Godinho, Catarina; Domingos, Josefa; Albuquerque, Luisa; Freitas, Vanda; Pereira, João Miguel; Cattoni, Begona; Carvalho, Herculano; Reimão, Sofia; Rosa, Mário M; Ferreira, António Gonalves; Ferreira, Joaquim J

2017-01-01

There is scarce data on the level of handicap in Parkinson's disease (PD) and none in advanced stage PD. To assess the handicap in advanced stage PD patients with disabling levodopa-induced motor complications selected to deep brain stimulation (DBS). Data was prospectively recorded during routine evaluation for DBS. Handicap was measured using London Handicap Scale (LHS) (0 = maximal handicap; 1 = no handicap). Disease severity was evaluated using the Hoehn & Yahr scale and the UPDRS/MDS-UPDRS, during off and on after a supra-maximal dose of levodopa. Schwab and England Scale (S&E) was scored in off and on. Dyskinesias were scored using the modified Abnormal Involuntary Movement Scale (mAIMS). Results concern cross-sectional assessment before DBS. 100 PD patients (mean age 61 (±7.6); mean disease duration 12.20 (±4.6) years) were included. Median score of motor MDS-UPDRS was 54 in off and 25 in on. Mean total LHS score was 0.56 (±0.14). Patients were handicapped in several domains with a wide range of severity. Physical Independence and Social Integration were the most affected domains. Determinants of total LHS score were MDS-UPDRS part II off (β= -0.271; p = 0.020), S&E on (β= 0.264; p = 0.005) and off (β= 0.226; p = 0.020), and mAIMS on (β= -0.183; p = 0.042) scores (R2  = 29.6%). We were able to use handicap to measure overall health condition in advanced stage PD. Patients were moderately to highly handicapped and this was strongly determined by disability in ADL and dyskinesias. Change in handicap may be a good patient-centred outcome to assess efficiency of DBS.

Identification of critical regions in human SAMHD1 required for nuclear localization and Vpx-mediated degradation.

PubMed

Guo, Haoran; Wei, Wei; Wei, Zhenhong; Liu, Xianjun; Evans, Sean L; Yang, Weiming; Wang, Hong; Guo, Ying; Zhao, Ke; Zhou, Jian-Ying; Yu, Xiao-Fang

2013-01-01

The sterile alpha motif (SAM) and HD domain-containing protein-1 (SAMHD1) inhibits the infection of resting CD4+ T cells and myeloid cells by human and related simian immunodeficiency viruses (HIV and SIV). Vpx inactivates SAMHD1 by promoting its proteasome-dependent degradation through an interaction with CRL4 (DCAF1) E3 ubiquitin ligase and the C-terminal region of SAMHD1. However, the determinants in SAMHD1 that are required for Vpx-mediated degradation have not been well characterized. SAMHD1 contains a classical nuclear localization signal (NLS), and NLS point mutants are cytoplasmic and resistant to Vpx-mediated degradation. Here, we demonstrate that NLS-mutant SAMHD1 K11A can be rescued by wild-type SAMHD1, restoring its nuclear localization; consequently, SAMHD1 K11A became sensitive to Vpx-mediated degradation in the presence of wild-type SAMHD1. Surprisingly, deletion of N-terminal regions of SAMHD1, including the classical NLS, generated mutant SAMHD1 proteins that were again sensitive to Vpx-mediated degradation. Unlike SAMHD1 K11A, these deletion mutants could be detected in the nucleus. Interestingly, NLS-defective SAMHD1 could still bind to karyopherin-β1 and other nuclear proteins. We also determined that the linker region between the SAM and HD domain and the HD domain itself is important for Vpx-mediated degradation but not Vpx interaction. Thus, SAMHD1 contains an additional nuclear targeting mechanism in addition to the classical NLS. Our data indicate that multiple regions in SAMHD1 are critical for Vpx-mediated nuclear degradation and that association with Vpx is not sufficient for Vpx-mediated degradation of SAMHD1. Since the linker region and HD domain may be involved in SAMHD1 multimerization, our results suggest that SAMHD1 multimerization may be required for Vpx-mediation degradation.

The Gamma-Ray Emitting Radio-Loud Narrow-Line Seyfert 1 Galaxy PKS 2004-447 II. The Radio View

NASA Technical Reports Server (NTRS)

Schulz, R.; Kreikenbohm, A.; Kadler, M.; Ojha, R.; Ros, E.; Stevens, J.; Edwards, P. G.; Carpenter, B.; Elsaesser, D.; Gehrels, N.;

Emirati Teachers' Perceptions of Voice Handicap.

PubMed

Natour, Yaser S; Sartawi, Abdealaziz M; Al Muhairy, Ousha; Efthymiou, Effie; Marie, Basem S

2016-05-01

The purpose of the study was to explore Emirati teachers' perceptions of voice handicap and to analyze their acoustic characteristics to determine whether acoustic measures of teachers' voice would verify their perceptions of voice handicap. Sixty-six Emirati school teachers (33 men and 33 women), with different years of teaching experience and age, and 100 control participants (50 men and 50 women) underwent vocal assessment that included the Voice Handicap Index (VHI-Arab) and acoustic measures (F0, jitter%, shimmer%, signal to noise ratio [SNR]). Significant differences between the teachers' group scores and the control group scores on the following subscales of VHI-Arab: physical (P = 0.006), emotional (P = 0.004), and total score of the test (P = 0.002). No significant differences were found among teachers in the three VHI subscales, and the total score regarding gender (functional P = 0.307; physical P = 0.341; emotional P = 0.126; and total P = 0.184), age (functional P = 0.972; physical P = 0.525; emotional P = 0.772; and total P = 0.848), and years of teaching experience (functional P = 0.319; physical P = 0.619; emotional P = 0.926; and total P = 0.638). The significant differences between the teacher's group and the control group in three acoustic measures: F0 (P = 0.000), shimmer% (P = 0.000), and SNR (P = 0.000) were further investigated. Significant differences were found among female and male teachers in F0 (P = 0.00) and SNR (P = 0.007). As for teachers' age, significant differences were found in SNR (P = 0.028). Teachers' years of experience did not show significant differences in any of the acoustic measures. Teachers have a higher perception of voice handicap. However, they were able to produce better voice quality than control participants were, as expressed in better SNRs. This might have been caused either by manipulation of vocal properties or abusive overloading the vocal system to produce a procedurally acceptable voice quality. Copyright © 2016 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

Physical Aspects of Residential Living 1972.

ERIC Educational Resources Information Center

Blakeley, Richard; And Others

The monograph presents four papers on the significance of the physical environment in residential facilities for retarded individuals which describe initial efforts to improve the physical characteristics of an institution serving a severely and profoundly retarded, as well as multiply handicapped, population. R. Scheerenberger considers the…

Microcomputers: Independence and Information Access for the Physically Handicapped.

ERIC Educational Resources Information Center

Regen, Shari S.; Chen, Ching-chih

1984-01-01

Provides overview of recent technological developments in microcomputer technology for the physically disabled, including discussion of view expansion, "talking terminals," voice recognition, and price and convenience of micro-based products. Equipment manufacturers and training centers for the physically disabled are listed and microcomputer…

[Fitness for automobile driving of physically handicapped patients].

PubMed

Ekkernkamp, A; Gerlach, D

1987-05-01

Some 30.5 million Germans hold a driving license. For the person with a physical handicap, too, active traffic participation is of special importance, in that it constitutes an essential link in his rehabilitation. Road traffic authorities, in view of traffic safety, are held to consider both, the handicapped individual's interest in obtaining a license and society's claim to safety. Numerous studies have established the fact that disabled drivers, in particular those with impairment of the limbs, are neither more accident-prone nor otherwise more conspicious in road traffic than the non-disabled. The view that the driver with a physical disability poses particular risks, has been invalidated by extensive factual data and thorough literature review. Diseases of major relevance for driving ability are touched on, the range of adaptive equipment is exemplified. Physicians in accident surgery primarily have to deal with trauma to the spinal cord and limbs. The majority of these conditions permit good compensation by technical devices, enabling safe driving of a private car. Awareness of legal/administrative aspects of licensing, as well as of the adaptive potential of technical aids are of great importance to both physician and client.

Training Manual for School Bus Drivers Transporting the Handicapped. [A Resource Manual for the Development and Evaluation of Special Programs for Exceptional Students, Volume IV-A].

ERIC Educational Resources Information Center

Florida State Dept. of Education, Tallahassee. Bureau of Education for Exceptional Students.

One of a series designed to help Florida school districts provide special programs for exceptional children, the training packet is intended to be used in a 2-day training session for school bus drivers who transport handicapped students. The manual includes a brief introduction to five disability areas (physical impairments, visual impairments,…

NLS Handbook, 2005. National Longitudinal Surveys

ERIC Educational Resources Information Center

Bureau of Labor Statistics, 2006

2006-01-01

The National Longitudinal Surveys (NLS), sponsored by the U.S. Bureau of Labor Statistics (BLS), are a set of surveys designed to gather information at multiple points in time on the labor market experiences of groups of men and women. Each of the cohorts has been selected to represent all people living in the United States at the initial…

Implications of NLS Data on Self-Esteem and Locus of Control for Psychological Research.

ERIC Educational Resources Information Center

Conger, A. J.; Costanzo, P. R.

During the course of the National Longitudinal Study of the High School Class of 1972 (NLS), a tremendous amount of data will be collected on the educational, vocational, and personal development of high school graduates, and the personal, familial, social, institutional, and cultural factors that contribute to that development. Information of a…

Some Remarks on Similarity and Soliton Solutions of Nonlinear Klein-Gordon Equation

NASA Astrophysics Data System (ADS)

Tajiri, Masayoshi

1984-11-01

The three-dimensional nonlinear Klein-Gordon [, Higgs field and Yang-Milles] (3D-KG [, H and YM]) equation is first reduced to the 2D nonlinear Schrödinger (2D-NLS) and 2D-KG [, H and YM] equations, and secondly to the 1D-NLS and 1D-KG [, H and YM] equations by similarity transformations. It is shown that similar type soliton solutions of the 3D-KG, H and YM equations, which have singularity on a plane in (x, y, z, t) space, are obtained by substituting the soliton solutions of the 1D-NLS or 1D-KG (or H) equation into the similarity transformations. The soliton solutions of the YM equation are also investigated.

Theoretical and experimental evidence of non-symmetric doubly localized rogue waves.

PubMed

He, Jingsong; Guo, Lijuan; Zhang, Yongshuai; Chabchoub, Amin

2014-11-08

We present determinant expressions for vector rogue wave (RW) solutions of the Manakov system, a two-component coupled nonlinear Schrödinger (NLS) equation. As a special case, we generate a family of exact and non-symmetric RW solutions of the NLS equation up to third order, localized in both space and time. The derived non-symmetric doubly localized second-order solution is generated experimentally in a water wave flume for deep-water conditions. Experimental results, confirming the characteristic non-symmetric pattern of the solution, are in very good agreement with theory as well as with numerical simulations, based on the modified NLS equation, known to model accurately the dynamics of weakly nonlinear wave packets in deep water.

Theoretical and experimental evidence of non-symmetric doubly localized rogue waves

PubMed Central

He, Jingsong; Guo, Lijuan; Zhang, Yongshuai; Chabchoub, Amin

2014-01-01

We present determinant expressions for vector rogue wave (RW) solutions of the Manakov system, a two-component coupled nonlinear Schrödinger (NLS) equation. As a special case, we generate a family of exact and non-symmetric RW solutions of the NLS equation up to third order, localized in both space and time. The derived non-symmetric doubly localized second-order solution is generated experimentally in a water wave flume for deep-water conditions. Experimental results, confirming the characteristic non-symmetric pattern of the solution, are in very good agreement with theory as well as with numerical simulations, based on the modified NLS equation, known to model accurately the dynamics of weakly nonlinear wave packets in deep water. PMID:25383023

On the relationship between the classical Dicke-Jaynes-Cummings-Gaudin model and the nonlinear Schroedinger equation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Du, Dianlou; Geng, Xue

2013-05-15

In this paper, the relationship between the classical Dicke-Jaynes-Cummings-Gaudin (DJCG) model and the nonlinear Schroedinger (NLS) equation is studied. It is shown that the classical DJCG model is equivalent to a stationary NLS equation. Moreover, the standard NLS equation can be solved by the classical DJCG model and a suitably chosen higher order flow. Further, it is also shown that classical DJCG model can be transformed into the classical Gaudin spin model in an external magnetic field through a deformation of Lax matrix. Finally, the separated variables are constructed on the common level sets of Casimir functions and the generalizedmore » action-angle coordinates are introduced via the Hamilton-Jacobi equation.« less

Identification of a novel NLS of herpes simplex virus type 1 (HSV-1) VP19C and its nuclear localization is required for efficient production of HSV-1.

PubMed

Li, You; Zhao, Lei; Wang, Shuai; Xing, Junji; Zheng, Chunfu

2012-09-01

Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production.

Developmental and Adapted Physical Education. Second Edition.

ERIC Educational Resources Information Center

Clarke, H. Harrison; Clarke, David H.

This book concentrates on activities to meet the needs of children and adults who are deficient in basic physical fitness components, who are handicapped in some respect: posture faults, poor flexibility, weight problems, or other inadequacies which interfere with their effective participation in physical education activities. It stresses…

45 CFR 1151.11 - Handicapped person.

Code of Federal Regulations, 2011 CFR

2011-10-01

... who has a physical or mental impairment that substantially limits one or more major life activities... activities. (4) Is regarded as having an impairment means: (i) Has a physical or mental impairment that does..., the phrase: (1) Physical or mental impairment means: (i) Any physiological disorder or condition...

38 CFR 18.403 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... physical impairment that substantially limits one or more major life activities. (iv) Is regarded as having... life activities but is treated by a recipient as constituting such a limitation; (B) Has a physical or... person. (1) Handicapped person means any person who: (i) Has a physical or mental impairment which...

38 CFR 18.403 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... physical impairment that substantially limits one or more major life activities. (iv) Is regarded as having... life activities but is treated by a recipient as constituting such a limitation; (B) Has a physical or... person. (1) Handicapped person means any person who: (i) Has a physical or mental impairment which...

Quasi-integrability in the modified defocusing non-linear Schrödinger model and dark solitons

NASA Astrophysics Data System (ADS)

Blas, H.; Zambrano, M.

2016-03-01

The concept of quasi-integrability has been examined in the context of deformations of the defocusing non-linear Schrödinger model (NLS). Our results show that the quasi-integrability concept, recently discussed in the context of deformations of the sine-Gordon, Bullough-Dodd and focusing NLS models, holds for the modified defocusing NLS model with dark soliton solutions and it exhibits the new feature of an infinite sequence of alternating conserved and asymptotically conserved charges. For the special case of two dark soliton solutions, where the field components are eigenstates of a space-reflection symmetry, the first four and the sequence of even order charges are exactly conserved in the scattering process of the solitons. Such results are obtained through analytical and numerical methods, and employ adaptations of algebraic techniques used in integrable field theories. We perform extensive numerical simulations and consider the scattering of dark solitons for the cubic-quintic NLS model with potential V=η {I}^2-in /6{I}^3 and the saturable type potential satisfying [InlineEquation not available: see fulltext.], with a deformation parameter ɛ ∈ [InlineMediaObject not available: see fulltext.] and I = | ψ|2. The issue of the renormalization of the charges and anomalies, and their (quasi)conservation laws are properly addressed. The saturable NLS supports elastic scattering of two soliton solutions for a wide range of values of { η, ɛ, q}. Our results may find potential applications in several areas of non-linear science, such as the Bose-Einstein condensation.

A hybrid approach to nanoelectronics

NASA Astrophysics Data System (ADS)

Cerofolini, G. F.; Arena, G.; Camalleri, C. M.; Galati, C.; Reina, S.; Renna, L.; Mascolo, D.

2005-08-01

The definition of features on the nanometre length scale (NLS) is impossible via conventional lithography, but can be done using extreme ultraviolet, synchrotron-radiation, or electron beam lithography. However, since these techniques are very expensive and still in their infancy, their exploitation in integrated circuit (IC) processing is still highly putative. Geometries on the NLS can however be produced with relative ease using the spacer patterning technique, i.e. transforming vertical features (like film thickness) in the vicinity of a step of a sacrificial layer into horizontal features. The ultimate length that can be produced in this way is controlled by the steepness of the step defining the sacrificial layer, the uniformity of the deposited or grown films, and the anisotropy of its etching. While useful for the preparation of a few devices with special needs, the above trick does not allow by itself the development of a nanotechnology where each layer useful for defining the circuit should be on the NLS and aligned on the underlying geometries with tolerances on the NLS. Setting up such a nanotechnology is a major problem which will involve the IC industry in the post-Roadmap era. Irrespective of the detailed structure of the basic constituents (molecules, supramolecular structures, clusters, etc), ICs with nanoscopic active elements can hardly be prepared without the ability to produce arrays of conductive strips with pitch on the NLS. This work is devoted to describing a scheme (essentially based on the existing microelectronic technology) for their production without the use of advanced lithography and how it can be arranged to host molecular devices.

Residual disabilities in children treated for intracranial space-occupying lesions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bamford, F.N.; Jones, P.M.; Pearson, D.

1976-02-01

A retrospective study of 30 long-term survivors of cranial or craniospinal irradiation for intracranial space-occupying lesions has demonstrated physical and mental handicaps in the majority. This is most severe in those cases treated before the age of 11 years, and is not confined to those children having craniospinal or whole-brain irradiation. The mental handicap also appears to be progressive, but cannot be easily examined in this retrospective study. (auth)

Accessibility to Laboratories and Equipment for the Physically Handicapped: A Handbook for Vocational Education Personnel.

ERIC Educational Resources Information Center

Erekson, Thomas L.; And Others

Intended to assist Illinois educational personnel to provide vocational education services for students with physical disabilities, the handbook's eight sections focus on program and facility modification to accommodate physically disabled students in regular vocational education programs. Section I introduces the topic, provides an historical…

Adapting Physical Education Activities.

ERIC Educational Resources Information Center

Bundschuh, Ernest; And Others

Designed to meet the requirements of recent federal legislation, the booklet describes Project DART, which provides services in adapted physical education for handicapped children in Georgia. The first section examines the state of the art in adapted physical education and reviews the mandates of Public Law 94-142 (the Education for All…

Physical Education Through Movement in the City.

ERIC Educational Resources Information Center

Munz, Lorraine

The product of a Special Studies Institute, this teacher developed resource guide for the emotionally handicapped (K-6) presents concepts and activities relative to physical education in the urban out-of-doors. Focus is on adapting physical education to an urban environment, utilizing city resources and instilling skills necessary to cope with…

Book of Proceedings of Workshops Held on Physical Education for the Mentally Retarded.

ERIC Educational Resources Information Center

Mississippi Univ. for Women, Columbus.

Proceedings are presented from three workshops concerning physical education for the mentally retarded held at Mississippi State University, University of Mississippi, and University of Southern Mississippi in 1967. Topics covered in the workshops include program development of physical education with the mentally handicapped, reports of research…

Aqua Dynamics. Physical Conditioning through Water Exercises.

ERIC Educational Resources Information Center

President's Council on Physical Fitness and Sports, Washington, DC.

Swimming is recognized as America's most popular active sport. It is one of the best physical activities for people of all ages and for people who are physically handicapped. Vigorous water exercises can increase a person's flexibility, strength, and cardio-vascular endurance. Exercises requiring flexibility are performed more easily in water…

5 CFR 900.703 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... physical impairment that substantially limits one or more major life activities. (4) Is regarded as having... life activities but is treated by a recipient as constituting such a limitation; (ii) Has a physical or... or interest in such property. (d) Handicapped person means any person who has a physical or mental...

NLS-72-Postsecondary Education Transcript Files. Data User's Manual for Revised Transcript, Term, and Course Files. Supplementary Addendum.

ERIC Educational Resources Information Center

Adelman, Clifford

This addendum describes the nature, rationale, and results of the second cleaning and revision of the National Longitudinal Study of 1972 (NLS-72) Postsecondary Education Transcript Files (PETS). This project, conducted for nearly 3 years, followed an initial cleaning and reduction in 1987, which came about as a reaction to the idiosyncratic…

New Data Available for the National Longitudinal Surveys.

ERIC Educational Resources Information Center

Morgan, William R.

The National Longitudinal surveys (NLS) of Labor Market Behavior have been conducted by the Center for Human Resource Research at Ohio State University and supported by the Department of Labor since l966. In this paper, data from the fifth and newest NLS cohort, a national cohort of 12,686 youth who were aged 14-21 in 1979, are discussed.…

46 CFR 151.12-10 - Operation of oceangoing non-self-propelled ships Carrying Category D NLS.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 5 2010-10-01 2010-10-01 false Operation of oceangoing non-self-propelled ships Carrying Category D NLS. 151.12-10 Section 151.12-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Equipment and Operating Requirements for Control of...

Efficiency Study of NLS Base-Year Design. RTI-22U-884-3.

ERIC Educational Resources Information Center

Moore, R. P.; And Others

An efficiency study was conducted of the base year design used for the National Longitudinal Study of the High School Class of 1972 (NLS). Finding the optimal design involved a search for the numbers of sample schools and students that would maximize the variance at a given cost. Twenty-one variables describing students' plans, attitudes,…

46 CFR 153.470 - System for discharge of NLS residue to the sea: Categories A, B, C, and D.

Code of Federal Regulations, 2012 CFR

2012-10-01

... (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SHIPS CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS... discharge of NLS residue to the sea: Categories A, B, C, and D. Unless waived under § 153.491, each ship... cubic meters per hour at which the ship operator wishes to discharge slops (note: Qd affects the...