Long-range attraction of an ultrarelativistic electron beam by a column of neutral plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adli, Erik; Lindstrom, C. A.; Allen, J.

Here, we report on the experimental observation of the attraction of a beam of ultrarelativistic electrons towards a column of neutral plasma. In experiments performed at the FACET test facility at SLAC we observe that an electron beam moving parallel to a neutral plasma column, at an initial distance of many plasma column radii, is attracted into the column. Once the beam enters the plasma it drives a plasma wake similar to that of an electron beam entering the plasma column head-on. A simple analytical model is developed in order to capture the essential physics of the attractive force. Themore » attraction is further studied by 3D particle-in-cell numerical simulations. The results are an important step towards better understanding of particle beam–plasma interactions in general and plasma wakefield accelerator technology in particular.« less

Low-frequency instabilities and plasma turbulence

NASA Technical Reports Server (NTRS)

Ilic, D. B.

1973-01-01

A theoretical and experimental study is reported of steady-state and time-dependent characteristics of the positive column and the hollow cathode discharge (HCD). The steady state of a non-isothermal, cylindrical positive column in an axial magnetic field is described by three moment equations in the plasma approximation. Volume generation of electron-ion pairs by single-stage ionization, the presence of axial current, and collisions with neutrals are considered. The theory covers the range from the low pressure, collisionless regime to the intermediate pressure, collisional regime. It yields radial profiles of the charged particle velocities, density, potential, electron and ion temperatures, and demonstrates similarity laws for the positive column. The results are compared with two moment theories and with experimental data on He, Ar and Hg found in the literature for a wide range of pressures. A simple generalization of the isothermal theory for an infinitely long cylinder in an axial magnetic field to the case of a finite column with axial current flow is also demonstrated.

Nonlinear wave interaction in a plasma column

NASA Technical Reports Server (NTRS)

Larsen, J.

1972-01-01

Two particular cases of nonlinear wave interaction in a plasma column were investigated. The frequencies of the waves were on the order of magnitude of the electron plasma frequency, and ion motion was neglected. The nonlinear coupling of slow waves on a plasma column was studied by means of cold plasma theory, and the case of a plasma column surrounded by an infinite dielectric in the absence of a magnetic field was also examined. Nonlinear scattering from a plasma column in an electromagnetic field having it's magnetic field parallel to the axis of the column was investigated. Some experimental results on mode conversion in the presence of loss are presented along with some observations of nonlinear scattering, The effect of the earth's magnetic field and of discharge symmetry on the radiation pattern are discussed.

Direct-injection screening for acidic drugs in plasma and neutral drugs in equine urine by differential-gradient LC-LC coupled MS/MS.

PubMed

Stanley, Shawn M R; Wee, Wei Khee; Lim, Boon Huat; Foo, Hsiao Ching

2007-04-01

Direct-injection LC-LC hybrid tandem MS methods have been developed for undertaking broad-based screening for acidic drugs in protein-precipitated plasma and neutral doping agents in equine urine. In both analyses, analytes present in the matrix were trapped using a HLB extraction column before being refocused and separated on a Chromolith RP-18e monolithic analytical column using a controlled differential gradient generated by proportional dilution of the first column's eluent with water. Each method has been optimised by the adoption of a mobile phase and gradient that was tailored to enhance ionisation in the MS source while maintaining good chromatographic behaviour for the majority of the target drugs. The analytical column eluent was fed into the heated nebulizer (HN) part of the Duospray interface attached to a 4000 QTRAP mass spectrometer. Information dependent acquisition (IDA) with dynamic background subtraction (DBS) was configured to trigger a sensitive enhanced product ion (EPI) scan when a multiple reaction monitoring (MRM) survey scan signal exceeded the defined criteria. Ninety-one percent of acidic drugs in protein-precipitated plasma and 80% of the neutral compounds in equine urine were detected when spiked at 10 ng/ml.

STELLARATOR INJECTOR

DOEpatents

Post, R.F.

1962-09-01

A method and means are described for injecting energetic neutral atoms or molecular ions into dense magnetically collimated plasma columns of stellarators and the like in such a manner that the atoms or ions are able to significantly penetrate the column before being ionized by collision with the plasma constituent particles. Penetration of the plasma column by the neutral atoms or molecular ions is facilitated by superposition of two closely spaced magnetic mirrors on the plasma confinement field. The mirrors are moved apart to magnetically sweep plasma from a region between the mirrors and establish a relatively low plasma density therein. By virture of the low density, neutral atoms or molecular ions injected into the region significantly penetrate the plasma column before being ionized. Thereafter, the mirrors are diminished to permit the injected material to admix with the plasma in the remainder of the column. (AEC)

Hydrodynamic study of plasma amplifiers for soft-x-ray lasers: a transition in hydrodynamic behavior for plasma columns with widths ranging from 20 μm to 2 mm.

PubMed

Oliva, Eduardo; Zeitoun, Philippe; Velarde, Pedro; Fajardo, Marta; Cassou, Kevin; Ros, David; Sebban, Stephan; Portillo, David; le Pape, Sebastien

2010-11-01

Plasma-based seeded soft-x-ray lasers have the potential to generate high energy and highly coherent short pulse beams. Due to their high density, plasmas created by the interaction of an intense laser with a solid target should store the highest amount of energy density among all plasma amplifiers. Our previous numerical work with a two-dimensional (2D) adaptive mesh refinement hydrodynamic code demonstrated that careful tailoring of plasma shapes leads to a dramatic enhancement of both soft-x-ray laser output energy and pumping efficiency. Benchmarking of our 2D hydrodynamic code in previous experiments demonstrated a high level of confidence, allowing us to perform a full study with the aim of the way for 10-100 μJ seeded soft-x-ray lasers. In this paper, we describe in detail the mechanisms that drive the hydrodynamics of plasma columns. We observed transitions between narrow plasmas, where very strong bidimensional flow prevents them from storing energy, to large plasmas that store a high amount of energy. Millimeter-sized plasmas are outstanding amplifiers, but they have the limitation of transverse lasing. In this paper, we provide a preliminary solution to this problem.

Plasma Source Development for LAPD

NASA Astrophysics Data System (ADS)

Pribyl, P.; Gekelman, W.; Drandell, M.; Grunspen, S.; Nakamoto, M.; McBarron, A.

2003-10-01

The Large Plasma Device (LAPD) relies on an indirectly heated Barium Oxide (BaO) cathode to generate an extremely repeatable low-noise plasma. However there are two defects of this system: one is that the cathode is subject to oxygen poisoning in the event of accidental air leaks, requiring a lengthy recoating and regeneration process. Second, the indirect radiative heating is only about 50 % efficient, leading to a series of reliability issues. Alternate plasma sources are being investigated, including two types of directly heated BaO cathode and several configurations of inductively coupled RF plasmas. Direct heating for a cathode can be achieved either by embedding heaters within the nickel substrate, or by using inductive heating techniques to drive currents within the nickel itself. In both cases, the BaO coating still serves to emit the electrons and thus generate the plasma arc. An improved system would generate the plasma without the use of a "cathode" e.g. by inductively coupling energy directly into the plasma discharge. This technique is being investigated from the point of view of whether a) the bulk of the plasma column can be made sufficiently low-noise to be of experimental value and b) sufficiently dense plasmas can be formed.

Effect of turbulent flow on an atmospheric-pressure AC powered gliding arc discharge

NASA Astrophysics Data System (ADS)

Kong, Chengdong; Gao, Jinlong; Zhu, Jiajian; Ehn, Andreas; Aldén, Marcus; Li, Zhongshan

2018-06-01

A high-power gliding arc (GA) discharge was generated in a turbulent air flow driven by a 35 kHz alternating current electric power supply. The effects of the flow rate on the characteristics of the GA discharge were investigated using combined optical and electrical diagnostics. Phenomenologically, the GA discharge exhibits two types of discharge, i.e., glow type and spark type, depending on the flow rates and input powers. The glow-type discharge, which has peak currents of hundreds of milliamperes, is sustained at low flow rates. The spark-type discharge, which is characterized by a sharp current spike of several amperes with duration of less than 1 μs, occurs more frequently as the flow rate increases. Higher input power can suppress spark-type discharges in moderate turbulence, but this effect becomes weak under high turbulent conditions. Physically, the transition between glow- and spark-type is initiated by the short cutting events and the local re-ignition events. Short cutting events occur owing to the twisting, wrinkling, and stretching of the plasma columns that are governed by the relatively large vortexes in the flow. Local re-ignition events, which are defined as re-ignition along plasma columns, are detected in strong turbulence due to increment of the impedance of the plasma column and consequently the internal electric field strength. It is suggested that the vortexes with length scales smaller than the size of the plasma can penetrate into the plasma column and promote mixing with surroundings to accelerate the energy dissipation. Therefore, the turbulent flow influences the GA discharges by ruling the short cutting events with relatively large vortexes and the local re-ignition events with small vortexes.

Study of scattering cross section of a plasma column using Green's function volume integral equation method

NASA Astrophysics Data System (ADS)

Soltanmoradi, Elmira; Shokri, Babak

2017-05-01

In this article, the electromagnetic wave scattering from plasma columns with inhomogeneous electron density distribution is studied by the Green's function volume integral equation method. Due to the ready production of such plasmas in the laboratories and their practical application in various technological fields, this study tries to find the effects of plasma parameters such as the electron density, radius, and pressure on the scattering cross-section of a plasma column. Moreover, the incident wave frequency influence of the scattering pattern is demonstrated. Furthermore, the scattering cross-section of a plasma column with an inhomogeneous collision frequency profile is calculated and the effect of this inhomogeneity is discussed first in this article. These results are especially used to determine the appropriate conditions for radar cross-section reduction purposes. It is shown that the radar cross-section of a plasma column reduces more for a larger collision frequency, for a relatively lower plasma frequency, and also for a smaller radius. Furthermore, it is found that the effect of the electron density on the scattering cross-section is more obvious in comparison with the effect of other plasma parameters. Also, the plasma column with homogenous collision frequency can be used as a better shielding in contrast to its inhomogeneous counterpart.

Scaling of Turbulence and Transport with ρ* in LAPD

NASA Astrophysics Data System (ADS)

Guice, Daniel; Carter, Troy; Rossi, Giovanni

2014-10-01

The plasma column size of the Large Plasma Device (LAPD) is varied in order to investigate the variation of turbulence and transport with ρ* =ρs / a . The data set includes plasmas produced by the standard BaO plasma source (straight field plasma radius a 30 cm) as well as the new higher density, higher temperature LaB6 plasma source (straight field plasma radius a 10 cm). The size of the plasma column is scaled in order to observe a Bohm to Gyro-Bohm diffusion transition. The main plasma column magnetic field is held fixed while the field in the cathode region is changed in order to map the cathode to different plasma column scales in the main chamber. Past experiments in the LAPD have shown a change in the observed diffusion but no transition to Gyro-Bohm diffusion. Results will be presented from an ongoing campaign to push the LAPD into the Gyro-Bohm diffusion regime.

Existence of a return direction for plasma escaping from a pinched column in a plasma focus discharge

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kubes, P.; Cikhardt, J.; Klir, D.

2015-05-15

The use of multi-frame interferometry used on the PF-1000 device with the deuterium filling showed the existence of a return motion of the top of several lobules of the pinched column formed at the pinched plasma column. This phenomenon was observed in the presence of an over-optimal mass in front of the anode, which depressed the intensity of the implosion and the smooth surface of the pinched plasma column. The observed evolution was explored through the use of closed poloidal currents transmitted outside the pinched plasma. This interpretation complements the scenario of the closed currents flowing within the structures insidemore » the pinched column, which has been published recently on the basis of observations from interferometry, neutron, and magnetic probe diagnostics on this device.« less

Negative ion source development for a photoneutralization based neutral beam system for future fusion reactors

NASA Astrophysics Data System (ADS)

Simonin, A.; Agnello, R.; Bechu, S.; Bernard, J. M.; Blondel, C.; Boeuf, J. P.; Bresteau, D.; Cartry, G.; Chaibi, W.; Drag, C.; Duval, B. P.; de Esch, H. P. L.; Fubiani, G.; Furno, I.; Grand, C.; Guittienne, Ph; Howling, A.; Jacquier, R.; Marini, C.; Morgal, I.

2016-12-01

In parallel to the developments dedicated to the ITER neutral beam (NB) system, CEA-IRFM with laboratories in France and Switzerland are studying the feasibility of a new generation of NB system able to provide heating and current drive for the future DEMOnstration fusion reactor. For the steady-state scenario, the NB system will have to provide a high NB power level with a high wall-plug efficiency (η ˜ 60%). Neutralization of the energetic negative ions by photodetachment (so called photoneutralization), if feasible, appears to be the ideal solution to meet these performances, in the sense that it could offer a high beam neutralization rate (>80%) and a wall-plug efficiency higher than 60%. The main challenge of this new injector concept is the achievement of a very high power photon flux which could be provided by 3 MW Fabry-Perot optical cavities implanted along the 1 MeV D- beam in the neutralizer stage. The beamline topology is tall and narrow to provide laminar ion beam sheets, which will be entirely illuminated by the intra-cavity photon beams propagating along the vertical axis. The paper describes the present R&D (experiments and modelling) addressing the development of a new ion source concept (Cybele source) which is based on a magnetized plasma column. Parametric studies of the source are performed using Langmuir probes in order to characterize and compare the plasma parameters in the source column with different plasma generators, such as filamented cathodes, radio-frequency driver and a helicon antenna specifically developed at SPC-EPFL satisfying the requirements for the Cybele (axial magnetic field of 10 mT, source operating pressure: 0.3 Pa in hydrogen or deuterium). The paper compares the performances of the three plasma generators. It is shown that the helicon plasma generator is a very promising candidate to provide an intense and uniform negative ion beam sheet.

Analysis of staged Z-pinch implosion trajectories from experiments on Zebra

NASA Astrophysics Data System (ADS)

Ross, Mike P.; Conti, F.; Darling, T. W.; Ruskov, E.; Valenzuela, J.; Wessel, F. J.; Beg, F.; Narkis, J.; Rahman, H. U.

2017-10-01

The Staged Z-pinch plasma confinement concept relies on compressing an annular liner of high-Z plasma onto a target plasma column of deuterium fuel. The interface between the liner and target is stable against the Magneto-Rayleigh-Taylor Instability, which leads to effective fuel compression and makes the concept interesting as a potential fusion reactor. The liner initiates as a neutral gas puff, while the target plasma is a partially ionized (Zeff < 10 percent column ejected from a coaxial plasma gun. The Zebra pulsed power generator (1 MA peak current, 100 ns rise time) provides the discharge that ionizes the liner and drives the Z-pinch implosion. Diverse diagnostics observe the 100-300 km/s implosions including silicon diodes, photo-conducting detectors (PCDs), laser shadowgraphy, an XUV framing camera, and a visible streak camera. The imaging diagnostics track instabilities smaller than 0.1 mm, and Z-pinch diameters below 2.5 mm are seen at peak compression. This poster correlates the data from these diagnostics to elucidate implosion behavior dependencies on liner gas, liner pressure, target pressure, and applied, axial-magnetic field. Funded by the Advanced Research Projects Agency - Energy, DE-AR0000569.

Monitoring Temperature in High Enthalpy Arc-heated Plasma Flows using Tunable Diode Laser Absorption Spectroscopy

NASA Technical Reports Server (NTRS)

Martin, Marcel Nations; Chang, Leyen S.; Jeffries, Jay B.; Hanson, Ronald K.; Nawaz, Anuscheh; Taunk, Jaswinder S.; Driver, David M.; Raiche, George

2013-01-01

A tunable diode laser sensor was designed for in situ monitoring of temperature in the arc heater of the NASA Ames IHF arcjet facility (60 MW). An external cavity diode laser was used to generate light at 777.2 nm and laser absorption used to monitor the population of electronically excited oxygen atoms in an air plasma flow. Under the assumption of thermochemical equilibrium, time-resolved temperature measurements were obtained on four lines-of-sight, which enabled evaluation of the temperature uniformity in the plasma column for different arcjet operating conditions.

Generation and confinement of microwave gas-plasma in photonic dielectric microstructure.

PubMed

Debord, B; Jamier, R; Gérôme, F; Leroy, O; Boisse-Laporte, C; Leprince, P; Alves, L L; Benabid, F

2013-10-21

We report on a self-guided microwave surface-wave induced generation of ~60 μm diameter and 6 cm-long column of argon-plasma confined in the core of a hollow-core photonic crystal fiber. At gas pressure of 1 mbar, the micro-confined plasma exhibits a stable transverse profile with a maximum gas-temperature as high as 1300 ± 200 K, and a wall-temperature as low as 500 K, and an electron density level of 10¹⁴ cm⁻³. The fiber guided fluorescence emission presents strong Ar⁺ spectral lines in the visible and near UV. Theory shows that the observed combination of relatively low wall-temperature and high ionisation rate in this strongly confined configuration is due to an unprecedentedly wide electrostatic space-charge field and the subsequent ion acceleration dominance in the plasma-to-gas power transfer.

Fluid modeling of radical species generation mechanism in dense methane-air mixture streamer discharge

NASA Astrophysics Data System (ADS)

Qian, Muyang; Li, Gui; Kang, Jinsong; Liu, Sanqiu; Ren, Chunsheng; Zhang, Jialiang; Wang, Dezhen

2018-01-01

Atmospheric dielectric barrier discharge (DBD) was found to be promising in the context of plasma chemistry, plasma medicine, and plasma-assisted combustion. In this paper, we present a detailed fluid modeling study of abundant radical species produced by a positive streamer in atmospheric dense methane-air DBD. A two-dimensional axisymmetric fluid model is constructed, in which 82 plasma chemical reactions and 30 different species are considered. Spatial and temporal density distributions of dominant radicals and ions are presented. We lay our emphasis on the effect of varying relative permittivity (ɛr = 2, 4.5, and 9) on the streamer dynamics in the plasma column, such as electric field behavior, production, and destruction pathways of dominant radical species. We find that higher relative permittivity promotes propagation of electric field and formation of conduction channel in the plasma column. The streamer discharge is sustained by the direct electron-impact ionization of methane molecule. Furthermore, the electron-impact dissociation of methane (e + CH4 = >e + H+CH3) is found to be the dominant reaction pathway to produce CH3 and H radicals. Similarly, the electron-impact dissociations of oxygen (e + O2 = >e + O+O(1D), e + O2 = >e + O+O) are the major routes for O production.

Plasma expansion into a waveguide created by a linearly polarized femtosecond laser pulse

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lemos, N.; Grismayer, T.; Cardoso, L.

2013-06-15

We demonstrate the efficient generation of 4 mm and 8 mm long plasma waveguides in hydrogen and helium. These waveguides have matching spots sizes for 13 to 34 μm laser beams. The plasma waveguides are created by ultra-short laser pulses (sub-picosecond) of moderate intensities, ∼10{sup 15}–10{sup 16} W cm{sup −2}, that heat the plasma to initial temperatures of tens of eV in order to create a hot plasma column that will expand into a plasma waveguide. We have determined that the main heating mechanism when using fs laser pulses and plasma densities ∼10{sup 18–19} cm{sup −3} is Above Threshold Ionization.more » Detailed time and space electron density measurements are presented for the laser produced plasma waveguides.« less

Apparatus and method for plasma processing of SRF cavities

NASA Astrophysics Data System (ADS)

Upadhyay, J.; Im, Do; Peshl, J.; Bašović, M.; Popović, S.; Valente-Feliciano, A.-M.; Phillips, L.; Vušković, L.

2016-05-01

An apparatus and a method are described for plasma etching of the inner surface of superconducting radio frequency (SRF) cavities. Accelerator SRF cavities are formed into a variable-diameter cylindrical structure made of bulk niobium, for resonant generation of the particle accelerating field. The etch rate non-uniformity due to depletion of the radicals has been overcome by the simultaneous movement of the gas flow inlet and the inner electrode. An effective shape of the inner electrode to reduce the plasma asymmetry for the coaxial cylindrical rf plasma reactor is determined and implemented in the cavity processing method. The processing was accomplished by moving axially the inner electrode and the gas flow inlet in a step-wise way to establish segmented plasma columns. The test structure was a pillbox cavity made of steel of similar dimension to the standard SRF cavity. This was adopted to experimentally verify the plasma surface reaction on cylindrical structures with variable diameter using the segmented plasma generation approach. The pill box cavity is filled with niobium ring- and disk-type samples and the etch rate of these samples was measured.

Spatial structures arising along a surface wave produced plasma column: an experimental study

NASA Astrophysics Data System (ADS)

Atanassov, V.; Mateev, E.

2007-04-01

The formation of spatial structures in high-frequency and microwave discharges has been known for several decades. Nevertheless it still raises increased interest, probably due to the variety of the observed phenomena and the lack of adequate and systematic theoretical interpretation. In this paper we present preliminary results on observation of spatial structures appearing along a surface wave sustained plasma column. The experiments have been performed in noble gases (xenon and neon) at low to intermediate pressure and the surface wave has been launched by a surfatron. Under these conditions we have observed and documented: i) appearance of stationary plasma rings; ii) formation of standing-wave striationlike patterns; iii) contraction of the plasma column; iv) plasma column transition into moving plasma balls and filaments. Some of the existing theoretical considerations of these phenomena are reviewed and discussed.

3D Global Fluid Simulations of Turbulence in LAPD

NASA Astrophysics Data System (ADS)

Rogers, Barrett; Ricci, Paolo; Li, Bo

2009-05-01

We present 3D global fluid simulations of the UCLA upgraded Large Plasma Device (LAPD). This device confines an 18-m-long, cylindrically symmetric plasma with a uniform magnetic field. The plasma in the simulations is generated by density and temperature sources inside the computational domain, and sheath boundary conditions are applied at the ends of the plasma column. In 3D simulations of the entire plasma, we observe strong, rotating intermittent density and temperature fluctuations driven by resistive driftwave turbulence with finite parallel wavenumbers. Analogous simulations carried out in the 2D limit (that is, assuming that the motions are purely interchange-like) display much weaker mode activity driven a Kelvin-Helmholtz instability. The properties and scaling of the turbulence and transport will be discussed.

Absorption of laser plasma in competition with oscillation currents for a terahertz spectrum.

PubMed

Li, Xiaolu; Bai, Ya; Li, Na; Liu, Peng

2018-01-01

We generate terahertz radiation in a supersonic jet of nitrogen molecules pumped by intense two-color laser pulses. The tuning of terahertz spectra from blue shift to red shift is observed by increasing laser power and stagnation pressure, and the red shift range is enlarged with the increased stagnation pressure. Our simulation reveals that the plasma absorption of the oscillation currents and expanded plasma column owing to increased laser intensity and gas number density are crucial factors in the recurrence of the red shift of terahertz spectra. The findings disclose the microscopic mechanism of terahertz radiation and present a controlling knob for the manipulation of a broadband terahertz spectrum from laser plasma.

A Multiple Z-Pinch Configuration for the Generation of High-Density, Magnetized Plasmas

NASA Astrophysics Data System (ADS)

Tarditi, Alfonso G.

2015-11-01

The z-pinch is arguably the most straightforward and economical approach for the generation and confinement of hot plasmas, with a long history of theoretical investigations and experimental developments. While most of the past studies were focused on countering the natural tendency of z-pinches to develop instabilities, this study attempts to take advantage of those unstable regimes to form a quasi-stable plasma, with higher density and temperature, possibly of interest for a fusion reactor concept. For this purpose, a configuration with four z-pinch discharges, with axis parallel to each other and symmetrically positioned, is considered. Electrodes for the generation of the discharges and magnetic coils are arranged to favor the formation of concave discharge patterns. The mutual attraction from the co-streaming discharge currents enhances this pattern, leading to bent plasma streams, all nearing towards the axis. This configuration is intended to excite and sustain a ``kink'' unstable mode for each z-pinch, eventually producing either plasmoid structures, detached from each discharge, or sustained kink patterns: both these cases appear to lead to plasmas merging in the central region. The feasibility of this approach in creating a higher density, hotter, meta-stable plasma regime is investigated computationally, addressing both the kink excitation phase and the dynamics of the converging plasma columns.

Investigation of flows in LAPD and their relation to edge turbulence and intermittency

NASA Astrophysics Data System (ADS)

Schaffner, D.; Carter, T. A.; Friedman, B.; Vincena, S.; Auerbach, D. W.; Popovich, P.

2009-11-01

We report on measurements of spontaneous flows and turbulence in the Large Plasma Device (LAPD) at UCLA. Measurements of perpendicular and parallel flow using a six-sided Mach probe reveal edge-localized perpendicular flows. The source of this flow is under investigation and may be generated by boundary effects or turbulent processes. Particular cases where a plasma depletion zone is created, including inserting a blocking disk within the cathode region and forming a compressed column, are used to analyze the effects on plasma flows. Ultimately, the relationship between the flows, turbulence and intermittency---the formation of blobs---is sought.

MHD Instabilities and Toroidal Field Effects on Plasma Column Behavior in Tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Khorshid, Pejman; Plasma Physics Research Center, Islamic Azad University, 14665-678, Tehran; Wang, L.

2006-12-04

In the edge plasma of the CT-6B and IRAN-T1 tokamaks the shape of plasma column based on MHD behavior has been studied. The bulk of plasma behavior during plasma column rotation as non-rigid body plasma has been investigated. We found that mode number and rotation frequency of plasma column are different in angle position, so that the mode number detected from Mirnov coils array located in poloidal angle on the inner side of chamber is more than outer side which it can be because of toroidal magnetic field effects. The results of IR-T1 and CT-6B tokamaks compared with each other,more » so that in the CT-6B because of its coils number must be less, but because of its Iron core the effect of toroidal magnetic field became more effective with respect to IR-T1. In addition, it is shown that the plasma column behaves as non-Rigid body plasma so that the poloidal rotation velocity variation in CT-6B is more than IR-T1. A relative correction for island rotation frequency has been suggested in connection with IRAN-T1 and CT-6B tokamak results, which can be considered for optical measurement purposes and also for future advanced tokamak control design.« less

Tailoring the charged particle fluxes across the target surface of Magnum-PSI

NASA Astrophysics Data System (ADS)

Costin, C.; Anita, V.; Popa, G.; Scholten, J.; De Temmerman, G.

2016-04-01

Linear plasma generators are plasma devices designed to study fusion-relevant plasma-surface interactions. The first requirement for such devices is to operate with adjustable and well characterized plasma parameters. In the linear plasma device Magnum-PSI, the distribution of the charged particle flux across the target surface can be tailored by the target bias. The process is based on the radial inhomogeneity of the plasma column and it is evidenced by electrical measurements via a 2D multi-probe system installed as target. Typical results are reported for a hydrogen discharge operated at 125 A and confined by a magnetic field strength of 0.95 T in the middle of the coils. The probes were biased in the range of  -80 to  -25 V, while the floating potential of the target was about  -35 V. The results were obtained in steady-state regime of Magnum-PSI, being time-averaged over any type of fluctuations. Depending on the relative value of the target bias voltage with respect to the local floating potential in the plasma column, the entire target surface can be exposed to ion or electron dominated flux, respectively, or it can be divided into two adjacent zones: one exposed to electron flux and the other to ion flux. As a consequence of this effect, a floating conductive surface that interacts with an inhomogeneous plasma is exposed to non-zero local currents despite its overall null current and it is subjected to internal current flows.

Stability of a Plasma Column. Free-Particle Model; STABILITE D'UNE COLONNE DE PLASMA. MODELE DES PARTICULES LIBRES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Troyon, F.

1963-12-01

The stability of a field-free homogeneous column of plasma confined to an axial static field and the sum of an alternating and static B/sub tt/ field is considered in the freeparticle model. Conditions for the existence of a positive average restoring force are derived, and it is shown that for small deformations the column is stable for sufficientiy high frequency. (auth)

PLASMA ENERGIZATION

DOEpatents

Furth, H.P.; Chambers, E.S.

1962-03-01

BS>A method is given for ion cyclotron resonance heatthg of a magnetically confined plasma by an applied radio-frequency field. In accordance with the invention, the radiofrequency energy is transferred to the plasma without the usual attendent self-shielding effect of plasma polarlzatlon, whereby the energy transfer is accomplished with superior efficiency. More explicitly, the invention includes means for applying a radio-frequency electric field radially to an end of a plasma column confined in a magnetic mirror field configuration. The radio-frequency field propagates hydromagnetic waves axially through the column with the waves diminishing in an intermediate region of the column at ion cyclotron resonance with the fleld frequency. In such region the wave energy is converted by viscous damping to rotational energy of the plasma ions. (AEC)

Radial dependence of HF wave field strength in the BPD column. [Beam Plasma Discharge

NASA Technical Reports Server (NTRS)

Jost, R. J.; Anderson, H. R.; Bernstein, W.; Kellogg, P. J.

1982-01-01

The results of a recent set of RF frequency measurements of the beam plasma discharge (BPD) performed in order to determine a quantitative value for the field strength in the plasma frequency region of the spectrum are presented. The parallel and perpendicular components of the plasma wave electric fields inside the BPD column have comparable field strengths, on the order of 10 volts/m. The radial dependence of the field strength is very strong, decreasing by as much as 40 dB within one meter from the beam center, with the illumination or discharge column approximately one meter in diameter. The field strength inside the column increases as a function of distance along the beam at least for several meters from the gun aperture. The frequency and amplitude of the plasma wave increases with beam current. A particularly rapid increase in these parameters occurs as the beam current approaches the critical current.

Harnessing mass differential confinement effects in magnetized rotating plasmas to address new separation needs

NASA Astrophysics Data System (ADS)

Gueroult, R.; Rax, J.-M.; Zweben, S. J.; Fisch, N. J.

2018-01-01

The ability to separate large volumes of mixed species based on atomic mass appears desirable for a variety of emerging applications with high societal impact. One possibility to meet this objective consists in leveraging mass differential effects in rotating plasmas. Beyond conventional centrifugation, rotating plasmas offer in principle additional ways to separate elements based on mass. Single ion orbits show that ion radial mass separation in a uniform magnetized plasma column can be achieved by applying a tailored electric potential profile across the column, or by driving a rotating magnetic field within the column. Furthermore, magnetic pressure and centrifugal effects can be combined in a non-uniform geometry to separate ions based on mass along the field lines. Practical application of these separation schemes hinges on the ability to produce the desirable electric and magnetic field configuration within the plasma column.

Extreme degree of ionization in homogenous micro-capillary plasma columns heated by ultrafast current pulses.

PubMed

Avaria, G; Grisham, M; Li, J; Tomasel, F G; Shlyaptsev, V N; Busquet, M; Woolston, M; Rocca, J J

2015-03-06

Homogeneous plasma columns with ionization levels typical of megaampere discharges are created by rapidly heating gas-filled 520-μm-diameter channels with nanosecond rise time current pulses of 40 kA. Current densities of up to 0.3  GA cm^{-2} greatly increase Joule heating with respect to conventional capillary discharge Z pinches, reaching unprecedented degrees of ionization for a high-Z plasma column heated by a current pulse of remarkably low amplitude. Dense xenon plasmas are ionized to Xe^{28+}, while xenon impurities in hydrogen discharges reach Xe^{30+}. The unique characteristics of these hot, ∼300:1 length-to-diameter aspect ratio plasmas allow the observation of unexpected spectroscopic phenomena. Axial spectra show the unusual dominance of the intercombination line over the resonance line of He-like Al by nearly an order of magnitude, caused by differences in opacities in the axial and radial directions. These plasma columns could enable the development of sub-10-nm x-ray lasers.

Broadband sidebands generated by parametric instability in lower hybrid current drive experiments on EAST

NASA Astrophysics Data System (ADS)

Amicucci, L.; Ding, B. J.; Castaldo, C.; Cesario, R.; Giovannozzi, E.; Li, M. H.; Tuccillo, A. A.

2015-12-01

Modern research on nuclear fusion energy, based on the tokamak concept, has strong need of tools for actively driving non-inductive current especially at the periphery of plasma column, where tools available so far have poor efficiency. This is essential for solving one of the most critical problems for thermonuclear reactor, consisting in how to achieve the figure of fusion gain in the context of sufficient stability. The lower hybrid current drive (LHCD) effect has the potential capability of driving current at large radii of reactor plasma with high efficiency [1]. Experiments recently carried out on EAST showed that a strong activity of LH sideband waves (from the RF probe spectra), accompanied by weak core penetration of the coupled LH power, is present when operating at relatively high plasma densities. Previous theoretical results, confirmed by experiments on FTU, showed that the LH sideband phenomenon is produced by parametric instability (PI), which are mitigated by higher plasma edge temperatures. This condition is thus useful for enabling the LH power propagation when operating with profiles having high plasma densities even at the edge. In the present work, we show new PI modeling of EAST plasmas data, obtained in condition of higher plasma edge temperature due to chamber lithisation. The obtained trend of the PI frequencies and growth rates is consistent with data of RF probe spectra, available in different regimes of lithisated and not lithisated vessel. Moreover, these spectra are interpreted as PI effect occurring at the periphery of plasma column, however in the low field side where the LH power is coupled.

Investigation of the helicon discharge plasma parameters in a hybrid RF plasma system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aleksandrov, A. F.; Petrov, A. K., E-mail: alpetrov57@gmail.com; Vavilin, K. V.

2016-03-15

Results of an experimental study of the helicon discharge plasma parameters in a prototype of a hybrid RF plasma system equipped with a solenoidal antenna are described. It is shown that an increase in the external magnetic field leads to the formation of a plasma column and a shift of the maximum ion current along the discharge axis toward the bottom flange of the system. The shape of the plasma column can be controlled via varying the configuration of the magnetic field.

Influence of the Al wire placed in the anode axis on the transformation of the deuterium plasma column in the plasma focus discharge

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kubes, P.; Cikhardtova, B.; Cikhardt, J.

In this paper, we describe the influence of an Al wire of 270 μm in diameter placed along the anode axis on the transformation of the deuterium pinch column in a megaampere (MA) plasma focus device. The evolution of the pinched column and of the wire corona was investigated by means of the multiframe interferometry, neutron and X-ray diagnostics. The wire corona did not influence considerably on the evolution of dense plasma structures and neutron production, but it increased the plasma density and consequently, the currents around its surface. The distribution of the closed internal currents (ranging hundreds of kA) andmore » associated magnetic fields amounting to 5 T were also estimated in the dense plasma column and in plasmoidal structures at the near-equilibrium state. The description is based on the balance of the plasma pressure and the pressure of the internal poloidal and toroidal current components compressed by the external pinched column. The dominant number of fusion deuterium-deuterium (D-D) neutrons is produced during the evolution of instabilities, when the uninterrupted wire corona (containing deuterium) connects the dense structures of the pinch, and it did not allow the formation of a constriction of the sub-millimeter diameter.« less

Dramatic enhancement of supercontinuum generation in elliptically-polarized laser filaments

PubMed Central

Rostami, Shermineh; Chini, Michael; Lim, Khan; Palastro, John P.; Durand, Magali; Diels, Jean-Claude; Arissian, Ladan; Baudelet, Matthieu; Richardson, Martin

2016-01-01

Broadband laser sources based on supercontinuum generation in femtosecond laser filamentation have enabled applications from stand-off sensing and spectroscopy to the generation and self-compression of high-energy few-cycle pulses. Filamentation relies on the dynamic balance between self-focusing and plasma defocusing – mediated by the Kerr nonlinearity and multiphoton or tunnel ionization, respectively. The filament properties, including the supercontinuum generation, are therefore highly sensitive to the properties of both the laser source and the propagation medium. Here, we report the anomalous spectral broadening of the supercontinuum for filamentation in molecular gases, which is observed for specific elliptical polarization states of the input laser pulse. The resulting spectrum is accompanied by a modification of the supercontinuum polarization state and a lengthening of the filament plasma column. Our experimental results and accompanying simulations suggest that rotational dynamics of diatomic molecules play an essential role in filamentation-induced supercontinuum generation, which can be controlled with polarization ellipticity. PMID:26847427

Observations of temperature rise during electron cyclotron heating application in Proto-MPEX

NASA Astrophysics Data System (ADS)

Biewer, T. M.; Bigelow, T.; Caneses, J. F.; Diem, S. J.; Rapp, J.; Reinke, M.; Kafle, N.; Ray, H. B.; Showers, M.

2017-10-01

The Prototype Material Plasma Exposure eXperiment (Proto-MPEX) at ORNL utilizes a variety of power systems to generate and deliver a high heat flux plasma (1 MW/m2 for these discharges) onto the surface of material targets. In the experiments described here, up to 120 kW of 13.56 MHz ``helicon'' waves are combined with 20 kW of 28 GHz microwaves to produce Deuterium plasma discharges. The 28 GHz waves are launched in a region of the device where the magnetic field is axially varying near 0.8 T, resulting in the presence of a 2nd harmonic electron cyclotron heating (ECH) resonance layer that transects the plasma column. The electron density and temperature profiles are measured using a Thomson scattering (TS) diagnostic, and indicate that the electron density is radially peaked. In the core of the plasma column the electron density is higher than the cut-off density (0.9x1019 m-3) for ECH waves to propagate and O-X-B mode conversion into electron Bernstien waves (EBW) is expected. TS measurements indicate electron temperature increases during 28 GHz wave application, rising (from 5 eV to 20 eV) as the neutral Deuterium pressure is reduced below 1 mTorr. This work was supported by the US. D.O.E. contract DE-AC05-00OR22725.

Filamentation in the pinched column of the dense plasma focus

NASA Astrophysics Data System (ADS)

Kubes, P.; Paduch, M.; Cikhardt, J.; Cikhardtova, B.; Klir, D.; Kravarik, J.; Rezac, K.; Zielinska, E.; Sadowski, M. J.; Szymaszek, A.; Tomaszewski, K.; Zaloga, D.

2017-03-01

The paper describes the filamentary structure observed in the high-energy ultraviolet radiation for discharges performed at the hydrogen- or deuterium-filling and at the puffing of hydrogen, deuterium or helium, in a mega-ampere dense plasma-focus facility. The lifetime of this structure overcomes 50 ns. These filaments connect the surface of a pinched column with internal plasmoids formed at different combinations of filling and puffing gases and they should transport some current and plasma. During all the investigated deuterium shots, the fusion-produced neutrons were recorded. Therefore, deuterons should be present in the region of their acceleration, independent of the applied puffing of the gas. Simultaneously with the observed filaments, inside the dense plasma column small plasma-balls of mm-dimensions were observed, which had a similar lifetime (longer than the relaxation time) and quasi-stationary positions in the discharge volume. The observed filaments and balls might be a manifestation of the (i) discrete spatial structure of the current flowing through and around the dense plasma column and (ii) transport of the plasma from external layers to the central region. Their formation and visualization were easier due to the application of air admixtures in the puffed gas.

Plasma Channel Lenses and Plasma Tornadoes for Optical Beam Focusing and Transport

NASA Astrophysics Data System (ADS)

Hubbard, R. F.; Kaganovich, D.; Johnson, L. A.; Gordon, D. F.; Penano, J. R.; Hafizi, B.; Helle, M. H.; Mamonau, A. A.

2017-10-01

Shaped plasmas offer the possibility of manipulating laser pulses at intensities far above the damage limits for conventional optics. An example is the plasma channel, which is a cylindrical plasma column with an on-axis density minimum. Long plasma channels have been widely used to guide intense laser pulses, particularly in laser wakefield accelerators. A new concept, the ``plasma tornado'', offers the possibility of creating long plasma channels with no nearby structures and at densities lower than can be achieved by capillary discharges. A short plasma channel can focus a laser pulse in much the same manner as a conventional lens or off-axis parabola. When placed in front of the focal point of an intense laser pulse, a plasma channel lens (PCL) can reduce the effective f-number of conventional focusing optics. When placed beyond the focal point, it can act as a collimator. We will present experimental and modeling results for a new plasma tornado design, review experimental methods for generating short PCLs, and discuss potential applications. Supported by the Naval Research Laboratory Base Program.

Divertor for use in fusion reactors

DOEpatents

Christensen, Uffe R.

1979-01-01

A poloidal divertor for a toroidal plasma column ring having a set of poloidal coils co-axial with the plasma ring for providing a space for a thick shielding blanket close to the plasma along the entire length of the plasma ring cross section and all the way around the axis of rotation of the plasma ring. The poloidal coils of this invention also provide a stagnation point on the inside of the toroidal plasma column ring, gently curving field lines for vertical stability, an initial plasma current, and the shaping of the field lines of a separatrix up and around the shielding blanket.

Experiments on the Expansion of a Dense Plasma into a Background Magnetoplasma

NASA Astrophysics Data System (ADS)

Gekelman, Walter; Vanzeeland, Mike; Vincena, Steve; Pribyl, Pat

2003-10-01

There are many situations, which occur in space (coronal mass ejections, or are man-made (upper atmospheric detonations) as well as the initial stages of a supernovae, in which a dense plasma expands into a background magnetized plasma, that can support Alfvèn waves. The upgraded LArge Plasma Device (LAPD) is a machine, at UCLA, in which Alfvèn wave propagation in homogeneous and inhomogeneous plasmas has been studied. We describe a series of experiments,which involve the expansion of a dense (initially, n_laser-plasma/n_0≫1) laser-produced plasma into an ambient highly magnetized background plasma capable of supporting Alfvèn waves will be presented. The 150 MW laser is pulsed at the same 1 Hz repetition rate as the plasma in a highly reproducible experiment. The interaction results in the production of intense shear Alfvèn waves, as well as large density perturbations. The waves propagate away from the target and are observed to become plasma column resonances. In the initial phase the background magnetic field is expelled from a plasma bubble. Currents in the main body of the plasma are generated to neutralize the positively charged bubble. The current system which results, becomes that of a spectrum of shear Alfvèn waves. Spatial patterns of the wave magnetic fields waves are measured at over 10^4 locations. As the dense plasma expands across the magnetic field it seeds the column with shear waves. Most of the Alfvèn wave energy is in shear waves, which become field line resonances after a machine transit time. The interplay between waves, currents, inductive electric fields and space charge is analyzed in great detail. Dramatic movies of the measured wave fields and their associated currents will be presented. Work supported by ONR, and DOE /NSF.

Radial Profiles of the Plasma Electron Characteristics in a 30 kW Arc Jet

NASA Technical Reports Server (NTRS)

Codron, Douglas A.; Nawaz, Anuscheh

2013-01-01

The present effort aims to strengthen modeling work conducted at the NASA Ames Research Center by measuring the critical plasma electron characteristics within and slightly outside of an arc jet plasma column. These characteristics are intended to give physical insights while assisting in the formulation of boundary conditions to validate full scale simulations. Single and triple Langmuir probes have been used to achieve estimates of the electron temperature (T(sub e)), electron number density (n(sub e)) and plasma potential (outside of the plasma column) as probing location is varied radially from the flow centerline. Both the electron temperature and electron number density measurements show a large dependence on radial distance from the plasma column centerline with T(sub e) approx. = (3 - 12 eV and n(sub e) approx. = 10(exp 12) - 10(exp 14)/cu cm.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vincena, Stephen

The aim of the original proposal was a basic plasma study to experimentally investigate the fundamental physics of how dense, fast-flowing, and field-aligned jets of plasma couple energy and momentum to a much larger, ambient, magnetized plasma. Coupling channels that were explored included bulk plasma heating and flow generation; shock wave production; and wave radiation, particularly in the form of shear and compressional Alfvén waves. The wave radiation, particularly to shear Alfvén waves was successfully modeled using the 3D Particle-In-Cell code, OSIRIS. Experimentally, these jets were produced via pulsed Nd:YAG laser ablation of solid carbon (graphite) rods, which were immersedmore » in the main plasma column of the Large Plasma Device (LaPD) at UCLA’s Basic Plasma Science Facility (BaPSF.) The axial expansion of the laser-produced plasma (LPP) was supersonic and with parallel expansion speeds approximately equal to the Alfvén speed. The project was renewed and refocused efforts to then utilize the laser-produced plasmas as a tool for the disruption and reconnection of current sheets in magnetized plasmas« less

Non-linear wave interaction in a plasma column

NASA Technical Reports Server (NTRS)

Larsen, J.-M.; Crawford, F. W.

1979-01-01

Non-linear three-wave interaction is analysed for propagation along a cylindrical plasma column surrounded by an infinite dielectric, in the absence of a static magnetic field. An averaged-Lagrangian method is used, and the results are specialized to parametric interaction and mode conversion, assuming an undepleted pump wave. The theory for these two types of interactions is extended to include imperfect synchronism, and the effects of loss. Computations are presented indicating that parametric growth rates of the order of a fraction of a decibel per centimeter should be obtainable for plausible laboratory plasma column parameters.

Bernstein modes in a non-neutral plasma column

NASA Astrophysics Data System (ADS)

Walsh, Daniel; Dubin, Daniel H. E.

2018-05-01

This paper presents theory and numerical calculations of electrostatic Bernstein modes in an inhomogeneous cylindrical plasma column. These modes rely on finite Larmor radius effects to propagate radially across the column until they are reflected when their frequency matches the upper hybrid frequency. This reflection sets up an internal normal mode on the column and also mode-couples to the electrostatic surface cyclotron wave (which allows the normal mode to be excited and observed using external electrodes). Numerical results predicting the mode spectra, using a novel linear Vlasov code on a cylindrical grid, are presented and compared to an analytical Wentzel Kramers Brillouin (WKB) theory. A previous version of the theory [D. H. E. Dubin, Phys. Plasmas 20(4), 042120 (2013)] expanded the plasma response in powers of 1/B, approximating the local upper hybrid frequency, and consequently, its frequency predictions are spuriously shifted with respect to the numerical results presented here. A new version of the WKB theory avoids this approximation using the exact cold fluid plasma response and does a better job of reproducing the numerical frequency spectrum. The effect of multiple ion species on the mode spectrum is also considered, to make contact with experiments that observe cyclotron modes in a multi-species pure ion plasma [M. Affolter et al., Phys. Plasmas 22(5), 055701 (2015)].

Simultaneous determination of byak-angelicin and oxypeucedanin hydrate in rat plasma by column-switching high-performance liquid chromatography with ultraviolet detection.

PubMed

Ishihara, K; Fukutake, M; Asano, T; Mizuhara, Y; Wakui, Y; Yanagisawa, T; Kamei, H; Ohmori, S; Kitada, M

2001-04-05

A simple and sensitive column-switching HPLC method was developed for the simultaneous determination of two furocoumarin compounds, byak-angelicin and oxypeucedanin hydrate, which are the main components of hot water extract of Angelica dahurica root (AE), in rat plasma. Plasma sample was simply deproteinated with perchloric acid. After centrifugation, the supernatant was injected into a column-switching HPLC system consisting of a clean-up column (Symmetry Shield RP 8, 20x3.9 mm I.D.) and analytical column (Symmetry C18, 75x4.6 mm I.D.) which were connected with a six-port switching valve. The flow-rate of the mobile phase (acetonitrile-water, 20:80) was maintained at 1 ml/min. Detection was carried out at wavelength 260 nm with a UV detector. The column temperature was maintained at 40 degrees C. The calibration curves of byak-angelicin and oxypeucedanin hydrate were linear over the ranges 19.6 to 980 ng/ml (r2>0.997). The accuracy of these analytes was less than 4.4%. The intra- and inter-day relative standard deviations of byak-angelicin and oxypeucedanin hydrate were within 12.0% and 12.7%, respectively. The present method was applied for the analysis of plasma concentration from rats after administration of AE.

Tracing and separating plasma components causing matrix effects in hydrophilic interaction chromatography-electrospray ionization mass spectrometry.

PubMed

Ekdahl, Anja; Johansson, Maria C; Ahnoff, Martin

2013-04-01

Matrix effects on electrospray ionization were investigated for plasma samples analysed by hydrophilic interaction chromatography (HILIC) in gradient elution mode, and HILIC columns of different chemistries were tested for separation of plasma components and model analytes. By combining mass spectral data with post-column infusion traces, the following components of protein-precipitated plasma were identified and found to have significant effect on ionization: urea, creatinine, phosphocholine, lysophosphocholine, sphingomyelin, sodium ion, chloride ion, choline and proline betaine. The observed effect on ionization was both matrix-component and analyte dependent. The separation of identified plasma components and model analytes on eight columns was compared, using pair-wise linear correlation analysis and principal component analysis (PCA). Large changes in selectivity could be obtained by change of column, while smaller changes were seen when the mobile phase buffer was changed from ammonium formate pH 3.0 to ammonium acetate pH 4.5. While results from PCA and linear correlation analysis were largely in accord, linear correlation analysis was judged to be more straight-forward in terms of conduction and interpretation.

Determination of erythromycin in human plasma, using column liquid chromatography with a polymeric packing material, alkaline mobile phase and amperometric detection.

PubMed

Nilsson, L G; Walldorf, B; Paulsen, O

1987-12-25

A method based on column liquid chromatography was developed for determination of plasma concentrations of erythromycin. PRP-1, a polymeric type of packing material suitable for chromatography and amperometric detection at high pH, was used. The effect of pH on the column performance and on the electrochemical response was studied. A pH of ca. 10 was found to be optimal. After extraction with tert.-butyl methyl ether, plasma concentrations down to 0.2 mumol/l could be measured, using automated sample injection. Oleandomycin was used as internal standard. The method was used for determination of plasma concentrations in a pharmacokinetic study under steady-state conditions.

Non-linear wave interaction in a magnetoplasma column. I - Theory. II Experiment

NASA Technical Reports Server (NTRS)

Larsen, J.-M.; Crawford, F. W.

1979-01-01

The paper presents an analysis of non-linear three-wave interaction for propagation along a cylindrical plasma column surrounded either by a metallic boundary, or by an infinite dielectric, and immersed in an infinite, static, axial magnetic field. An averaged Lagrangian method is used and the results are specialized to parametric amplification and mode conversion, assuming an undepleted pump wave. Computations are presented for a magneto-plasma column surrounded by free space, indicating that parametric growth rates of the order of a fraction of a decibel per centimeter should be obtainable for plausible laboratory plasma parameters. In addition, experiments on non-linear mode conversion in a cylindrical magnetoplasma column are described. The results are compared with the theoretical predictions and good qualitative agreement is demonstrated.

Algebraic motion of vertically displacing plasmas

NASA Astrophysics Data System (ADS)

Pfefferlé, D.; Bhattacharjee, A.

2018-02-01

The vertical motion of a tokamak plasma is analytically modelled during its non-linear phase by a free-moving current-carrying rod inductively coupled to a set of fixed conducting wires or a cylindrical conducting shell. The solutions capture the leading term in a Taylor expansion of the Green's function for the interaction between the plasma column and the surrounding vacuum vessel. The plasma shape and profiles are assumed not to vary during the vertical drifting phase such that the plasma column behaves as a rigid body. In the limit of perfectly conducting structures, the plasma is prevented to come in contact with the wall due to steep effective potential barriers created by the induced Eddy currents. Resistivity in the wall allows the equilibrium point to drift towards the vessel on the slow timescale of flux penetration. The initial exponential motion of the plasma, understood as a resistive vertical instability, is succeeded by a non-linear "sinking" behaviour shown to be algebraic and decelerating. The acceleration of the plasma column often observed in experiments is thus concluded to originate from an early sharing of toroidal current between the core, the halo plasma, and the wall or from the thermal quench dynamics precipitating loss of plasma current.

Cold plasma technology close-up

USDA-ARS?s Scientific Manuscript database

This month’s column discusses cold plasma, an emerging technology that has potential applications as an antimicrobial process for fresh and fresh-cut fruits and vegetables, low-moisture foods, and food contact surfaces. Brendan A. Niemira, the coauthor of this month’s column, is the research leader ...

Characteristics of soft x-ray spectra from ultra-fast micro-capillary discharge plasmas

NASA Astrophysics Data System (ADS)

Li, Jing; Avaria, Gonzalo; Shlyaptsev, Vyacheslav; Tomasel, Fernando; Grisham, Michael; Dawson, Quincy; Rocca, Jorge; NSF CenterExtreme Ultraviolet Science; Technology Collaboration

2013-10-01

The efficient generation of high aspect ratio (e.g. 300:1) plasma columns ionized to very high degrees of ionization (e.g. Ni-like Xenon) by an ultrafast current pulses of moderate amplitude in micro-capillary channels is of interest for fundamental plasma studies and for applications such as the generation of discharge-pumped soft x-ray lasers. Spectra and simulations for plasmas generated in 500 um alumina capillary discharges driven by 35-40 kA current pulses with 4 ns rise time were obtained in Xenon and Neon discharges. The first shows the presence of lines corresponding to ionization stages up to Fe-like Xe. The latter show that Al impurities from the walls and Si (from injected SiH4) are ionized to the H-like and He-like stages. He-like spectra containing the resonance line significantly broaden by opacity, the intercombination line, and Li-like satellites are analyzed and modeled. For Xenon discharges, the spectral lines from the Ni-like transitions the 3d94d(3/2, 3/2)J=0 to the 3d94p(5/2, 3/2)J=1 and to 3d94p(3/2, 1/2)J=1 are observed at gas pressures up to 2.0 Torr. Work supported by NSF Award PHY-1004295.

X-ray emitting MHD accretion shocks in classical T Tauri stars. Case for moderate to high plasma-β values

NASA Astrophysics Data System (ADS)

Orlando, S.; Sacco, G. G.; Argiroffi, C.; Reale, F.; Peres, G.; Maggio, A.

2010-02-01

Context. Plasma accreting onto classical T Tauri stars (CTTS) is believed to impact the stellar surface at free-fall velocities, generating a shock. Current time-dependent models describing accretion shocks in CTTSs are one-dimensional, assuming that the plasma moves and transports energy only along magnetic field lines (β ≪ 1). Aims: We investigate the stability and dynamics of accretion shocks in CTTSs, considering the case of β ⪆ 1 in the post-shock region. In these cases the 1D approximation is not valid and a multi-dimensional MHD approach is necessary. Methods: We model an accretion stream propagating through the atmosphere of a CTTS and impacting onto its chromosphere by performing 2D axisymmetric MHD simulations. The model takes into account the stellar magnetic field, the gravity, the radiative cooling, and the thermal conduction (including the effects of heat flux saturation). Results: The dynamics and stability of the accretion shock strongly depend on the plasma β. In the case of shocks with β > 10, violent outflows of shock-heated material (and possibly MHD waves) are generated at the base of the accretion column and intensely perturb the surrounding stellar atmosphere and the accretion column itself (therefore modifying the dynamics of the shock). In shocks with β ≈ 1, the post-shock region is efficiently confined by the magnetic field. The shock oscillations induced by cooling instability are strongly influenced by β: for β > 10, the oscillations may be rapidly dumped by the magnetic field, approaching a quasi-stationary state, or may be chaotic with no obvious periodicity due to perturbation of the stream induced by the post-shock plasma itself; for β≈ 1 the oscillations are quasi-periodic, although their amplitude is smaller and the frequency higher than those predicted by 1D models. Three movies are only available in electronic form at http://www.aanda.org

High-throughput determination of faropenem in human plasma and urine by on-line solid-phase extraction coupled to high-performance liquid chromatography with UV detection and its application to the pharmacokinetic study.

PubMed

Xie, Rui; Wen, Jun; Wei, Hua; Fan, Guorong; Zhang, Dabing

2010-05-01

An automated system using on-line solid-phase extraction and HPLC with UV detection was developed for the determination of faropenem in human plasma and urine. Analytical process was performed isocratically with two reversed-phase columns connected by a switching valve. After simple pretreatment for plasma and urine with acetonitrile, a volume of 100microl upper layer of the plasma or urine samples was injected for on-line SPE column switching HPLC-UV analysis. The analytes were retained on the self-made trap column (Lichrospher C(18), 4.6mmx37mm, 25microm) with the loading solvent (20mM NaH(2)PO(4) adjusted pH 3.5) at flow rate of 2mlmin(-1), and most matrix materials were removed from the column to waste. After 0.5min washing, the valve was switched to another position so that the target analytes could be eluted from trap column to analytical column in the back-flush mode by the mobile phase (acetonitrile-20mM NaH(2)PO(4) adjusted pH 3.5, 16:84, v/v) at flow rate of 1.5mlmin(-1), and then separated on the analytical column (Ultimate XB-C(18), 4.6mmx50mm, 5microm).The complete cycle of the on-line SPE preconcentration purification and HPLC separation of the analytes was 5min. Calibration curves with good linearities (r=0.9994 for plasma sample and r=0.9988 for urine sample) were obtained in the range 0.02-5microgml(-1) in plasma and 0.05-10microg ml(-1) in urine for faropenem. The optimized method showed good performance in terms of specificity, linearity, detection and quantification limits, precision and accuracy. The method was successfully utilized to quantify faropenem in human plasma and urine to support the clinical pharmacokinetic studies. Copyright 2009 Elsevier B.V. All rights reserved.

Atomic oxygen beam source for erosion simulation

NASA Technical Reports Server (NTRS)

Cuthbertson, J. W.; Langer, W. D.; Motley, R. W.; Vaughn, J. A.

1991-01-01

A device for the production of low energy (3 to 10 eV) neutral atomic beams for surface modification studies is described that reproduces the flux of atomic oxygen in low Earth orbit. The beam is produced by the acceleration of plasma ions onto a negatively biased plate of high-Z metal; the ions are neutralized and reflected by the surface, retaining some fraction of their incident kinetic energy, forming a beam of atoms. The plasma is generated by a coaxial RF exciter which produces a magnetically-confined (4 kG) plasma column. At the end of the column, ions fall through the sheath to the plate, whose bias relative to the plasma can be varied to adjust the beam energy. The source provides a neutral flux approximately equal to 5 x 10(exp 16)/sq cm at a distance of 9 cm and a fluence approximately equal to 10(exp 20)/sq cm in five hours. The composition and energy of inert gas beams was diagnosed using a mass spectometer/energy analyzer. The energy spectra of the beams demonstrate energies in the range 5 to 15 eV, and qualitatively show expected dependences upon incident and reflecting atom species and potential drop. Samples of carbon film, carbon-based paint, Kapton, mylar, and teflon exposed to atomic O beams show erosion quite similar to that observed in orbit on the space shuttle.

Trivelpiece-Gould modes in a uniform unbounded plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stenzel, R. L.; Urrutia, J. M.

Trivelpiece-Gould (TG) modes originally described electrostatic surface waves on an axially magnetized cylindrical plasma column. Subsequent studies of electromagnetic waves in such plasma columns revealed two modes, a predominantly magnetic helicon mode (H) and the mixed magnetic and electrostatic Trivelpiece-Gould modes (TG). The latter are similar to whistler modes near the oblique cyclotron resonance in unbounded plasmas. The wave propagation in cylindrical geometry is assumed to be paraxial while the modes exhibit radial standing waves. The present work shows that TG modes also arise in a uniform plasma without radial standing waves. It is shown experimentally that oblique cyclotron resonancemore » arises in large mode number helicons. Their azimuthal wave number far exceeds the axial wave number which creates whistlers near the oblique cyclotron resonance. Cyclotron damping absorbs the TG mode and can energize electrons in the center of a plasma column rather than the edge of conventional TG modes. The angular orbital field momentum can produce new perpendicular wave-particle interactions.« less

Structure of the runaway electron loss during induced disruptions in TEXTOR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wongrach, K.; Finken, K. H.; Willi, O.

2015-10-15

The loss of runaway electrons during an induced disruption is recorded by a synchrotron imaging technique using a fast infrared CCD camera. The loss is predominantly diffuse. During the “spiky-loss phase”, when the runaway beam moves close to the wall, a narrow channel between the runaway column and a scintillator probe is formed and lasts until the runaway beam is terminated. In some cases, the processed images show a stripe pattern at the plasma edge. A comparison between the MHD dominated disruptions and the MHD-free disruption is performed. A new mechanism of plasma disruptions with the runaway electron generation andmore » a novel model which reproduces many characteristic features of the plasma beam evolution during a disruption is briefly described.« less

Reconnection Scaling Experiment (RSX): Magnetic Reconnection in Linear Geometry

NASA Astrophysics Data System (ADS)

Intrator, T.; Sovinec, C.; Begay, D.; Wurden, G.; Furno, I.; Werley, C.; Fisher, M.; Vermare, L.; Fienup, W.

2001-10-01

The linear Reconnection Scaling Experiment (RSX) at LANL is a new experiment that can create MHD relevant plasmas to look at the physics of magnetic reconnection. This experiment can scale many relevant parameters because the guns that generate the plasma and current channels do not depend on equilibrium or force balance for startup. We describe the experiment and initial electrostatic and magnetic probe data. Two parallel current channels sweep down a long plasma column and probe data accumulated over many shots gives 3D movies of magnetic reconnection. Our first data tries to define an operating regime free from kink instabilities that might otherwise confuse the data and shot repeatability. We compare this with MHD 2 fluid NIMROD simulations of the single current channel kink stability boundary for a variety of experimental conditions.

Hydrodynamic modelling of accretion impacts in classical T Tauri stars: radiative heating of the pre-shock plasma

NASA Astrophysics Data System (ADS)

Costa, G.; Orlando, S.; Peres, G.; Argiroffi, C.; Bonito, R.

2017-01-01

Context. It is generally accepted that, in classical T Tauri stars, the plasma from the circumstellar disc accretes onto the stellar surface with free-fall velocity and the impact generates a shock. The impact region is expected to contribute to emission in different spectral bands; many studies have confirmed that the X-rays arise from the post-shock plasma but, otherwise, there are no studies in the literature investigating the origin of the observed UV emission which is apparently correlated to accretion. Aims: We investigated the effect of radiative heating of the infalling material by the post-shock plasma at the base of the accretion stream, with the aim to identify in which region a significant part of the UV emission originates. Methods: We developed a one-dimensional hydrodynamic model describing the impact of an accretion stream onto the stellar surface; the model takes into account the gravity, the radiative cooling of an optically thin plasma, the thermal conduction, and the heating due to absorption of X-ray radiation. The latter term represents the heating of the infalling plasma due to the absorption of X-rays emitted from the post-shock region. Results: We found that the radiative heating of the pre-shock plasma plays a non-negligible role in the accretion phenomenon. In particular, the dense and cold plasma of the pre-shock accretion column is gradually heated up to a few 105K due to irradiation of X-rays arising from the shocked plasma at the impact region. This heating mechanism does not affect significantly the dynamics of the post-shock plasma. On the other hand, a region of radiatively heated gas (that we consider a precursor) forms in the unshocked accretion column and contributes significantly to UV emission. Our model naturally reproduces the luminosity of UV emission lines correlated to accretion and shows that most of the UV emission originates from the precursor.

Study of Pulsed vs. RF Plasma Properties for Surface Processing Applications

NASA Astrophysics Data System (ADS)

Tang, Ricky; Hopkins, Matthew; Barnat, Edward; Miller, Paul

2015-09-01

The ability to manipulate the plasma parameters (density, E/N) was previously demonstrated using a double-pulsed column discharge. Experiments extending this to large-surface plasmas of interest to the plasma processing community were conducted. Differences between an audio-frequency pulsed plasma and a radio-frequency (rf) discharge, both prevalent in plasma processing applications, were studied. Optical emission spectroscopy shows higher-intensity emission in the UV/visible range for the pulsed plasma comparing to the rf plasma at comparable powers. Data suggest that the electron energy is higher for the pulsed plasma leading to higher ionization, resulting in increased ion density and ion flux. Diode laser absorption measurements of the concentration of the 1S5 metastable and 1S4 resonance states of argon (correlated with the plasma E/N) provide comparisons between the excitation/ionization states of the two plasmas. Preliminary modeling efforts suggest that the low-frequency polarity switch causes a much more abrupt potential variation to support interesting transport phenomena, generating a ``wave'' of higher temperature electrons leading to more ionization, as well as ``sheath capture'' of a higher density bolus of ions that are then accelerated during polarity switch.

Nonlinear Generation of Electromagnetic Waves through Induced Scattering by Thermal Plasma.

PubMed

Tejero, E M; Crabtree, C; Blackwell, D D; Amatucci, W E; Mithaiwala, M; Ganguli, G; Rudakov, L

2015-12-09

We demonstrate the conversion of electrostatic pump waves into electromagnetic waves through nonlinear induced scattering by thermal particles in a laboratory plasma. Electrostatic waves in the whistler branch are launched that propagate near the resonance cone. When the amplitude exceeds a threshold ~5 × 10(-6) times the background magnetic field, wave power is scattered below the pump frequency with wave normal angles (~59°), where the scattered wavelength reaches the limits of the plasma column. The scattered wave has a perpendicular wavelength that is an order of magnitude larger than the pump wave and longer than the electron skin depth. The amplitude threshold, scattered frequency spectrum, and scattered wave normal angles are in good agreement with theory. The results may affect the analysis and interpretation of space observations and lead to a comprehensive understanding of the nature of the Earth's plasma environment.

Reconnection Scaling Experiment (RSX): Magnetic Reconnection in Linear Geometry

NASA Astrophysics Data System (ADS)

Intrator, T.; Sovinec, C.; Begay, D.; Wurden, G.; Furno, I.; Werley, C.; Fisher, M.; Vermare, L.; Fienup, W.

2001-10-01

The linear Reconnection Scaling Experiment (RSX) at LANL is a qualitatively different way of creating MHD relevant plasmas to look at the physics of magnetic reconnection. We show here an overview of the experiment and initial electrostatic and magnetic probe data. Plasma creation using plasma guns is independent of equilibrium or force balance, so we can scale many relevant parameters. As the magnetic reconnection region between two parallel current channels sweeps down a long plasma column we can generate 3D movies of magnetic reconnection from many repetitive shots. If two current channels were to move because of kink instabilities instead of mutual J x B forces and reconnection effects, each shot would less reproducible. Our data show the kink stability boundary for a single current channel. We compare this with MHD 2 fluid NIMROD simulations of the single current channel kink stability boundary for a variety of experimental conditions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Biewer, Theodore M.; Bigelow, Tim S.; Caneses Marin, Juan F.

The Prototype Material Plasma Exposure Experiment at the Oak Ridge National Laboratory utilizes a variety of power systems to generate and deliver a high heat flux plasma onto the surface of material targets. In the experiments described here, a deuterium plasma is produced via a ~100 kW, 13.56 MHz RF helicon source, to which ~20 kW of 28 GHz microwave power is applied. The electron density and temperature profiles are measured using a Thomson scattering (TS) diagnostic, and indicate that the electron density is centrally peaked. In the core of the plasma column, the electron density is higher than themore » cut-off density (~0.9 × 1019 m -3) for the launched mixture of X- and O-mode electron cyclotron heating waves to propagate. TS measurements indicate electron temperature increases from ~5 eV to ~20 eV during 28 GHz power application when the neutral deuterium pressure is reduced below 0.13 Pa (~1 mTorr.).« less

Separation of Albumin, Ceruloplasmin, and Transferrin from Human Plasma.

ERIC Educational Resources Information Center

Barnes, Grady; Frieden, Earl

1982-01-01

Procedures are provided for separating the principal metalloproteins (albumin, ceruloplasmin, and transferrin) from plasma using column chromatographic techniques. The experiment can be completed in two separate three-hour laboratory periods during which column chromatography is illustrated and the effect of pH on charge and affinity of a protein…

Collaborative Research. Atmospheric Pressure Microplasma Chemistry-Photon Synergies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, Sung-Jin; Eden, James Gary

Combining the effects of low temperature, atmospheric pressure microplasmas and microplasma photon sources offers the promise of greatly expanding the range of applications for each of them. The plasma sources create active chemical species and these can be activated further by the addition of photons and the associated photochemistry. There are many ways to combine the effects of plasma chemistry and photochemistry, especially if there are multiple phases present. This project combined the construction of appropriate test experimental systems, various spectroscopic diagnostics and mathematical modeling. Through a continuous discussion and co-design process with the UC-Berkeley Team, we have successfully completedmore » the fabrication and testing of all components for a microplasma array-assisted system designed for photon-activated plasma chemistry research. Microcavity plasma lamps capable of generating more than 20 mW/cm 2 at 172 nm (Xe dimer) were fabricated with a custom form factor to mate to the plasma chemistry setup, and a lamp was current being installed by the Berkeley team so as to investigate plasma chemistry-photon synergies at a higher photon energy (~7.2 eV) as compared to the UVA treatment that is afforded by UV LEDs operating at 365 nm. In particular, motivated by the promising results from the Berkeley team with UVA treatment, we also produced the first generation of lamps that can generate photons in the 300-370 nm wavelength range. Another set of experiments, conducted under the auspices of this grant, involved the use of plasma microjet arrays. The combination of the photons and excited radicals produced by the plasma column resulted in broad area deactivation of bacteria.« less

Post-pinch generation of electron beam in a low energy Mather-type plasma focus device

NASA Astrophysics Data System (ADS)

Behbahani, R. A.; Aghamir, F. M.; Aghamir

2013-10-01

The post-pinch generation of electron beam in a low energy Mather-type plasma focus (PF) device has been investigated. A fast-calibrated Rogowski coil was used to monitor the emission of electron beam. A two-channel diode X-ray spectrometer along with suitable filters provided the records of energy spectrum of X-ray radiation. Single time-period emissions of electron beam with duration of 100 to 20 ns were recorded in the high range of the device operating pressure (0.8-2 mbar). However, in the low range regime (0.2-0.8 mbar), occurrence of single spike electron beam with duration of 150 +/- 50 ns, as well as multi-emission of electrons with duration of 400 +/- 50 ns, was visible. A multi-peak of tube voltage along with multi-time-period radiation of X-rays dominated by copper lines (Cukα and Cukβ) was noticeable in the low-pressure range. The generated electron beam during the post-pinch phase of anomalous resistances is suspected to be the main source of X-ray radiation. This can also be related to the turbulence of the plasma column during the occurrence of anomalous resistances.

Algebraic motion of vertically displacing plasmas

DOE PAGES

Pfefferle, D.; Bhattacharjee, A.

2018-02-27

In this paper, the vertical motion of a tokamak plasma is analytically modelled during its non-linear phase by a free-moving current-carrying rod inductively coupled to a set of fixed conducting wires or a cylindrical conducting shell. The solutions capture the leading term in a Taylor expansion of the Green's function for the interaction between the plasma column and the surrounding vacuum vessel. The plasma shape and profiles are assumed not to vary during the vertical drifting phase such that the plasma column behaves as a rigid body. In the limit of perfectly conducting structures, the plasma is prevented to comemore » in contact with the wall due to steep effective potential barriers created by the induced Eddy currents. Resistivity in the wall allows the equilibrium point to drift towards the vessel on the slow timescale of flux penetration. The initial exponential motion of the plasma, understood as a resistive vertical instability, is succeeded by a non-linear “sinking” behaviour shown to be algebraic and decelerating. Finally, the acceleration of the plasma column often observed in experiments is thus concluded to originate from an early sharing of toroidal current between the core, the halo plasma, and the wall or from the thermal quench dynamics precipitating loss of plasma current.« less

Algebraic motion of vertically displacing plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pfefferle, D.; Bhattacharjee, A.

In this paper, the vertical motion of a tokamak plasma is analytically modelled during its non-linear phase by a free-moving current-carrying rod inductively coupled to a set of fixed conducting wires or a cylindrical conducting shell. The solutions capture the leading term in a Taylor expansion of the Green's function for the interaction between the plasma column and the surrounding vacuum vessel. The plasma shape and profiles are assumed not to vary during the vertical drifting phase such that the plasma column behaves as a rigid body. In the limit of perfectly conducting structures, the plasma is prevented to comemore » in contact with the wall due to steep effective potential barriers created by the induced Eddy currents. Resistivity in the wall allows the equilibrium point to drift towards the vessel on the slow timescale of flux penetration. The initial exponential motion of the plasma, understood as a resistive vertical instability, is succeeded by a non-linear “sinking” behaviour shown to be algebraic and decelerating. Finally, the acceleration of the plasma column often observed in experiments is thus concluded to originate from an early sharing of toroidal current between the core, the halo plasma, and the wall or from the thermal quench dynamics precipitating loss of plasma current.« less

Non-thermal plasma instabilities induced by deformation of the electron energy distribution function

NASA Astrophysics Data System (ADS)

Dyatko, N. A.; Kochetov, I. V.; Napartovich, A. P.

2014-08-01

Non-thermal plasma is a key component in gas lasers, microelectronics, medical applications, waste gas cleaners, ozone generators, plasma igniters, flame holders, flow control in high-speed aerodynamics and others. A specific feature of non-thermal plasma is its high sensitivity to variations in governing parameters (gas composition, pressure, pulse duration, E/N parameter). This sensitivity is due to complex deformations of the electron energy distribution function (EEDF) shape induced by variations in electric field strength, electron and ion number densities and gas excitation degree. Particular attention in this article is paid to mechanisms of instabilities based on non-linearity of plasma properties for specific conditions: gas composition, steady-state and decaying plasma produced by the electron beam, or by an electric current pulse. The following effects are analyzed: the negative differential electron conductivity; the absolute negative electron mobility; the stepwise changes of plasma properties induced by the EEDF bi-stability; thermo-current instability and the constriction of the glow discharge column in rare gases. Some of these effects were observed experimentally and some of them were theoretically predicted and still wait for experimental confirmation.

Plasma flow in peripheral region of detached plasma in linear plasma device

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hayashi, Y., E-mail: hayashi-yuki13@ees.nagoya-u.ac.jp; Ohno, N.; Kajita, S.

2016-01-15

A plasma flow structure is investigated using a Mach probe under detached plasma condition in a linear plasma device NAGDIS-II. A reverse flow along the magnetic field is observed in a steady-state at far-peripheral region of the plasma column in the upstream side from the recombination front. These experimental results indicate that plasma near the recombination front should strongly diffuse across the magnetic field, and it should be transported along the magnetic field in the reverse flow direction. Furthermore, bursty plasma density fluctuations associated with intermittent convective plasma transport are observed in the far-peripheral region of the plasma column inmore » both upstream and downstream sides from the recombination front. Such a nondiffusive transport can contribute to the intermittent reverse plasma flow, and the experimental results indicate that intermittent transports are frequently produced near the recombination front.« less

High performance liquid chromatographic method for the determination of cetirizine and ambroxol in human plasma and urine--a boxcar approach.

PubMed

Dharuman, J; Vasudhevan, M; Ajithlal, T

2011-09-01

A column switching high performance liquid chromatographic method with estimable sensitivity and accuracy was developed for the determination of cetirizine and ambroxol in human plasma using nebivolol as the internal standard. Plasma samples were prepared by liquid-liquid extraction in methylene chloride and a mixture of diethylether (80:20, v/v). The extracted samples were injected into a multifunctional clean-up column Supelcosil LCABZ (50 mm × 4.6 mm, 5 μm particle size) using mobile phase 1 comprising acetonitrile-phosphate buffer (pH 3.5; 20 mM) (20:80, v/v). The eluate of cetirizine and ambroxol were separated to an analytical Kromasil C(8) micro bore column (50 mm × 0.3 mm, 5 μm particle size) via a column switching device. A Kromasil C(18) analytical column (250 mm × 2.1 mm, 5 μm particle size) was used as a separation column. Mobile phase 2 consisting acetonitrile-triethylamine (0.5%) in phosphate buffer (pH 3.5; 20mM) (55:45, v/v) was used for the compound elution. The eluents were detected at 230 nm with photodiode array detector. An aliquot of 150 μl of plasma sample was introduced into the pretreatment column via the auto sampler using mobile phase 1 at a flow rate of 0.5 ml/min, column switching valve being positioned at A. The pretreatment column retained cetirizine, ambroxol and nebivolol (IS) in the column leaving the residual proteins of plasma eluted in void volume and drained out. The switching valve was shifted to position B at 7.5 min. Cetirizine, ambroxol and IS were eluted from the pretreatment column between 7. 5 and 11.5 min and introduced to the concentration column. Finally, cetirizine, ambroxol and IS were introduced to the separation column by switching valve using mobile phase 2 at a flow rate of 0.4 ml/min. During the analysis the pretreatment column was washed for the next analysis and resume to the position A. The total run time was 25 min for a sample. The procedure was repeated for urine analysis also. The method was linear from 2 to 450 ng/ml and 7-300 ng/ml for cetirizine and ambroxol respectively in plasma and 1-500 ng/ml and 5-400 ng/ml, respectively for cetirizine and ambroxol in urine. Intra-day and inter-day precision of cetirizine and ambroxol was below 15% in terms of coefficient of variation and accuracy of cetirizine and ambroxol was ranged from 94 to 101.6% and 91.1 to 100.2%, respectively. The method demonstrated high sensitivity and selectivity and therefore, applied to evaluate pharmacokinetics of cetirizine and ambroxol in healthy human volunteer after a single oral administration. Urine samples obtained from healthy human volunteers and clinical subjects with renal impairment have also been analyzed by the method to compare the elimination pattern. The method was precise and accurate for the estimation of cetirizine and ambroxol both in blood and in urine. Copyright © 2011 Elsevier B.V. All rights reserved.

Quantitative analysis of retinoids in biological fluids by high-performance liquid chromatography using column switching. I. Determination of isotretinoin and tretinoin and their 4-oxo metabolites in plasma.

PubMed

Wyss, R; Bucheli, F

1988-02-26

A fully automated gradient high-performance liquid chromatographic method for the determination of isotretinoin, tretinoin and their 4-oxo metabolites in plasma was developed, using the column-switching technique. After dilution with an internal standard solution containing 20% acetonitrile, 0.5 ml of the sample was injected onto a precolumn (17 X 4.6 mm I.D.), filled with C18 Corasil 37-53 micron. Proteins and polar plasma components were washed out using 1% ammonium acetate-acetonitrile (9:1, v/v) as mobile phase 1. After valve switching, the retained components were transferred to the analytical column in the backflush mode, separated by gradient elution and detected at 360 nm by UV detection. Using two coupled reversed-phase columns (125 mm long), the separation of cis and trans isomers was possible, and all four compounds could be quantified down to 2 ng/ml of plasma. The inter-assay precision in the concentration range 20-100 ng/ml was between 1.0 and 4.7% for all compounds.

Algebraic motion of vertically displacing plasmas

NASA Astrophysics Data System (ADS)

Bhattacharjee, Amitava; Pfefferle, David; Hirvijoki, Eero

2017-10-01

The vertical displacement of tokamak plasmas is modelled during the non-linear phase by a free-moving current-carrying rod coupled to a set of fixed conducting wires and a cylindrical conducting shell. The models capture the leading term in a Taylor expansion of the Green's function for the interaction between the plasma column and the vacuum vessel. The plasma is assumed not to vary during the VDE such that it behaves as a rigid body. In the limit of perfectly conducting structures, the plasma is prevented from coming in contact with the wall due to steep effective potential barriers by the eddy currents, and will hence oscillate at Alfvénic frequencies about a given force-free position. In addition to damping oscillations, resistivity allows for the column to drift towards the vessel on slow flux penetration timescales. The initial exponential motion of the plasma, i.e. the resistive vertical instability, is succeeded by a non-linear sinking behaviour, that is shown analytically to be algebraic and decelerative. The acceleration of the plasma column often observed in experiments is thus conjectured to originate from an early sharing of toroidal current between the core, the halo plasma and the wall or from the thermal quench dynamics precipitating loss of plasma current

High-energy photon spectra from a coaxial gas-puff experiment

NASA Astrophysics Data System (ADS)

Warren, S. W. R.; Degnan, J. H.; Beason, C. W.; Price, D. W.; Snell, M. P.

1987-04-01

An array of plastic scintillator/photomultiplier detectors was used to determine the high-energy (h x nu greater than 35 keV) photon spectra of a 130-kJ, 60-kV gas-injected coaxial-gun experiment. The detector array used six different filter-material/thickness combinations. High-energy photon signals were readily observed. The spectra were determined by deconvolution. The spectra have three characteristic components: radiative emission from high-Z impurities in the plasma, beam-target excited line emission, and thick-target bremsstrahlung from an electron beam generated in the plasma column. The electron beam consistent with the thick-target bremsstrahlung assumption was of the order of 100 A above 10 keV.

Aspartic acid incorporated monolithic columns for affinity glycoprotein purification.

PubMed

Armutcu, Canan; Bereli, Nilay; Bayram, Engin; Uzun, Lokman; Say, Rıdvan; Denizli, Adil

2014-02-01

Novel aspartic acid incorporated monolithic columns were prepared to efficiently affinity purify immunoglobulin G (IgG) from human plasma. The monolithic columns were synthesised in a stainless steel HPLC column (20 cm × 5 mm id) by in situ bulk polymerisation of N-methacryloyl-L-aspartic acid (MAAsp), a polymerisable derivative of L-aspartic acid, and 2-hydroxyethyl methacrylate (HEMA). Monolithic columns [poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid) (PHEMAsp)] were characterised by swelling studies, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The monolithic columns were used for IgG adsorption/desorption from aqueous solutions and human plasma. The IgG adsorption depended on the buffer type, and the maximum IgG adsorption from aqueous solution in phosphate buffer was 0.085 mg/g at pH 6.0. The monolithic columns allowed for one-step IgG purification with a negligible capacity decrease after ten adsorption-desorption cycles. Copyright © 2013 Elsevier B.V. All rights reserved.

Micro-column plasma emission liquid chromatograph

DOEpatents

Gay, Don D.

1984-01-01

In a direct current plasma emission spectrometer for use in combination with a micro-column liquid chromatograph, an improved plasma source unit. The plasma source unit includes a quartz capillary tube having an inlet means, outlet off gas means and a pair of spaced electrodes defining a plasma region in the tube. The inlet means is connected to and adapted to receive eluant of the liquid chromatograph along with a stream of plasma-forming gas. There is an opening through the wall of the capillary tube penetrating into the plasma region. A soft glass capillary light pipe is disposed at the opening, is connected to the spectrometer, and is adapted to transmit light passing from the plasma region to the spectrometer. There is also a source of electromotive force connected to the electrodes sufficient to initiate and sustain a plasma in the plasma region of the tube.

Radiative accretion shocks along nonuniform stellar magnetic fields in classical T Tauri stars

NASA Astrophysics Data System (ADS)

Orlando, S.; Bonito, R.; Argiroffi, C.; Reale, F.; Peres, G.; Miceli, M.; Matsakos, T.; Stehlé, C.; Ibgui, L.; de Sa, L.; Chièze, J. P.; Lanz, T.

2013-11-01

Context. According to the magnetospheric accretion model, hot spots form on the surface of classical T Tauri stars (CTTSs) in regions where accreting disk material impacts the stellar surface at supersonic velocity, generating a shock. Aims: We investigate the dynamics and stability of postshock plasma that streams along nonuniform stellar magnetic fields at the impact region of accretion columns. We study how the magnetic field configuration and strength determine the structure, geometry, and location of the shock-heated plasma. Methods: We model the impact of an accretion stream onto the chromosphere of a CTTS by 2D axisymmetric magnetohydrodynamic simulations. Our model considers the gravity, the radiative cooling, and the magnetic-field-oriented thermal conduction (including the effects of heat flux saturation). We explore different configurations and strengths of the magnetic field. Results: The structure, stability, and location of the shocked plasma strongly depend on the configuration and strength of the magnetic field. In the case of weak magnetic fields (plasma β ≳ 1 in the postshock region), a large component of B may develop perpendicular to the stream at the base of the accretion column, which limits the sinking of the shocked plasma into the chromosphere and perturbs the overstable shock oscillations induced by radiative cooling. An envelope of dense and cold chromospheric material may also develop around the shocked column. For strong magnetic fields (β < 1 in the postshock region close to the chromosphere), the field configuration determines the position of the shock and its stand-off height. If the field is strongly tapered close to the chromosphere, an oblique shock may form well above the stellar surface at the height where the plasma β ≈ 1. In general, we find that a nonuniform magnetic field makes the distribution of emission measure vs. temperature of the postshock plasma at T > 106 K lower than when there is uniform magnetic field. Conclusions: The initial magnetic field strength and configuration in the region of impact of the stream are expected to influence the chromospheric absorption and, therefore, the observability of the shock-heated plasma in the X-ray band. In addition, the field strength and configuration also influence the energy balance of the shocked plasma with its emission measure at T > 106 K, which is lower than expected for a uniform field. The above effects contribute to underestimating the mass accretion rates derived in the X-ray band. Movies are available in electronic form at http://www.aanda.org

A uniform laminar air plasma plume with large volume excited by an alternating current voltage

NASA Astrophysics Data System (ADS)

Li, Xuechen; Bao, Wenting; Chu, Jingdi; Zhang, Panpan; Jia, Pengying

2015-12-01

Using a plasma jet composed of two needle electrodes, a laminar plasma plume with large volume is generated in air through an alternating current voltage excitation. Based on high-speed photography, a train of filaments is observed to propagate periodically away from their birth place along the gas flow. The laminar plume is in fact a temporal superposition of the arched filament train. The filament consists of a negative glow near the real time cathode, a positive column near the real time anode, and a Faraday dark space between them. It has been found that the propagation velocity of the filament increases with increasing the gas flow rate. Furthermore, the filament lifetime tends to follow a normal distribution (Gaussian distribution). The most probable lifetime decreases with increasing the gas flow rate or decreasing the averaged peak voltage. Results also indicate that the real time peak current decreases and the real time peak voltage increases with the propagation of the filament along the gas flow. The voltage-current curve indicates that, in every discharge cycle, the filament evolves from a Townsend discharge to a glow one and then the discharge quenches. Characteristic regions including a negative glow, a Faraday dark space, and a positive column can be discerned from the discharge filament. Furthermore, the plasma parameters such as the electron density, the vibrational temperature and the gas temperature are investigated based on the optical spectrum emitted from the laminar plume.

Experimental identification of the kink instability as a poloidal flux amplification mechanism for coaxial gun spheromak formation.

PubMed

Hsu, S C; Bellan, P M

2003-05-30

The magnetohydrodynamic kink instability is observed and identified experimentally as a poloidal flux amplification mechanism for coaxial gun spheromak formation. Plasmas in this experiment fall into three distinct regimes which depend on the peak gun current to magnetic flux ratio, with (I) low values resulting in a straight plasma column with helical magnetic field, (II) intermediate values leading to kinking of the column axis, and (III) high values leading immediately to a detached plasma. Onset of column kinking agrees quantitatively with the Kruskal-Shafranov limit, and the kink acts as a dynamo which converts toroidal to poloidal flux. Regime II clearly leads to both poloidal flux amplification and the development of a spheromak configuration.

Demonstration of separate phosphotyrosyl- and phosphoseryl- histone phosphatase activities in the plasma membranes of a human astrocytoma.

PubMed

Leis, J F; Knowles, A F; Kaplan, N O

1985-06-01

A plasma membrane preparation from a human astrocytoma contained p-nitrophenyl phosphate (pNPP), phosphotyrosyl histone, and phosphoseryl histone hydrolysis activities. The pNPPase and phosphotyrosyl histone phosphatase activities were inhibited by vanadate, whereas the phosphoseryl histone phosphatase activity was not; the latter activity was inhibited by pyrophosphate and nucleoside di- and triphosphates. When the membranes were solubilized by Triton X-100 and the solubilized proteins were subjected to column chromatography on DEAE-Sephadex, Sepharose 6B-C1, and wheat germ agglutinin-Sepharose 4B columns, the pNPPase activity from the phosphoseryl histone phosphatase activity. The results from column chromatography also indicated that there may be multiple phosphotyrosyl and phosphoseryl protein phosphatases in the plasma membranes.

Differential coulometric oxidation following post column-switching high pressure liquid chromatography for fluorescence measurement of unmetabolized folic acid in human plasma.

PubMed

Bailey, Steven W; Ayling, June E

2013-11-08

Although many countries have fortified their grain supplies with folic acid (FA) to decrease the incidence of neural tube defects, others have not due to concerns that this synthetic folate might have some adverse effects. Persistent unmetabolized FA has been found even in plasma from fasted subjects. To facilitate measurement of low levels of folic acid in human plasma, post-column coulometric oxidative cleavage was used to convert poorly fluorescent FA into a highly fluorescent compound determined to be 6-formyl-pterin. To minimize sample work-up and maximize recovery, column-switching HPLC transferred a window of eluate containing the FA from the first column (C8) onto a second column (phenyl-hexyl). The pH of two mobile phases were adjusted to be above and then below a pK of the FA α-carboxyl group, thus promoting separation from compounds coeluting from the C8-column. This permitted sample preparation using only a simple high recovery protein precipitation. Definitive identification of FA in human plasma was accomplished by duplicate injections of sample with the electrochemical voltage set above and below its half-potential. The LOD (S/N=3) was 0.10 nM. The intra- and inter-assay CV's were 2.3% and 5%, respectively. Comparison of these results with those obtained by HPLC/MS/MS with stable isotope internal standard showed a slope of 1.00 ± 0.019. This simple, sensitive, and repeatable assay facilitates a more thorough investigation of the response of various human populations to folic acid intake. Post-column differential coulometric electrochemistry can expand the variety of compounds amenable to fluorescence detection. Copyright © 2013 Elsevier B.V. All rights reserved.

Excitation of THz hybrid modes in an elliptical dielectric rod waveguide with a cold collisionless unmagnetized plasma column by an annular electron beam

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rahmani, Z., E-mail: z.rahmani@kashanu.ac.ir; Safari, S.; Heidari-Semiromi, E.

2016-06-15

The dispersion relation of electromagnetic waves propagating in an elliptical plasma waveguide with a cold collisionless unmagnetized plasma column and a dielectric rod is studied analytically. The frequency spectrum of the hybrid waves and the growth rate for excitation of the waves by a thin annular relativistic elliptical electron beam (TAREEB) is obtained. The effects of relative permittivity constant of dielectric rod, geometrical dimensions, plasma frequency, accelerating voltage, and current density of TAREEB on the growth rate and frequency spectra of the waveguide will be investigated.

Separation of actinides using capillary extraction chromatography-inductively coupled plasma mass spectrometry.

PubMed

Peterson, Dominic S; Montoya, Velma M

2009-08-01

Trace levels of actinides have been separated on capillary extraction chromatography columns. Detection of the actinides was achieved using an inductively coupled plasma mass spectrometer, which was coupled with the extraction chromatography system. In this study, we compare 30-cm long, 4.6 mm i.d. columns to capillary columns (750 microm i.d.) with lengths from 30 cm up to 150 cm. The columns that were tested were packed with TRU resin. We were able to separate a mixture of five actinides ((232)Th, (238)U, (237)Np, (239)Pu, and (241)Am). This work has application to rapid bioassay as well as automated separations of actinide materials.

Current drive for stability of thermonuclear plasma reactor

NASA Astrophysics Data System (ADS)

Amicucci, L.; Cardinali, A.; Castaldo, C.; Cesario, R.; Galli, A.; Panaccione, L.; Paoletti, F.; Schettini, G.; Spigler, R.; Tuccillo, A.

2016-01-01

To produce in a thermonuclear fusion reactor based on the tokamak concept a sufficiently high fusion gain together stability necessary for operations represent a major challenge, which depends on the capability of driving non-inductive current in the hydrogen plasma. This request should be satisfied by radio-frequency (RF) power suitable for producing the lower hybrid current drive (LHCD) effect, recently demonstrated successfully occurring also at reactor-graded high plasma densities. An LHCD-based tool should be in principle capable of tailoring the plasma current density in the outer radial half of plasma column, where other methods are much less effective, in order to ensure operations in the presence of unpredictably changes of the plasma pressure profiles. In the presence of too high electron temperatures even at the periphery of the plasma column, as envisaged in DEMO reactor, the penetration of the coupled RF power into the plasma core was believed for long time problematic and, only recently, numerical modelling results based on standard plasma wave theory, have shown that this problem should be solved by using suitable parameter of the antenna power spectrum. We show here further information on the new understanding of the RF power deposition profile dependence on antenna parameters, which supports the conclusion that current can be actively driven over a broad layer of the outer radial half of plasma column, thus enabling current profile control necessary for the stability of a reactor.

Study of the characteristics of reconfigurable plasma antenna array

DOE Office of Scientific and Technical Information (OSTI.GOV)

Alias, Nur Salihah; Dagang, Ahmad Nazri; Ali, Mohd Tarmizi

This paper presents a design and simulation of a reconfigurable array of plasma antenna. The plasma column is used as radiating elements instead of metal to create an antenna. The advantages of the plasma antenna over the conventional antenna are its possible to change the operating parameters, such as the working pressure, input power, radius of the discharge tube, resonant frequency, and length of the plasma column. In addition, plasma antenna can be reconfigurable with respect to shape, frequency and radiation parameters in a very short time. The plasma discharge tube was designed with a length of 200 mm, the radiusmore » of the plasma column was 2.5 mm and the coupling sleeve was connected to the SMA as the ground. This simulation was performed by using the simulation software Computer Simulation Technology (CST). The frequency is set in the range of 1 GHz to 10 GHz. The performance of the designed antenna was analyzed in term of return loss, gain and radiation pattern. For reconfigurable plasma antenna array, it shows that the gain is increase when the number of antenna element is increase. The combination of the discharge tube and metal rod as an antenna array has been done, and the result shows that an array with the plasma element can achieve higher gain.« less

Pulsed, atmospheric pressure plasma source for emission spectrometry

DOEpatents

Duan, Yixiang; Jin, Zhe; Su, Yongxuan

2004-05-11

A low-power, plasma source-based, portable molecular light emission generator/detector employing an atmospheric pressure pulsed-plasma for molecular fragmentation and excitation is described. The average power required for the operation of the plasma is between 0.02 W and 5 W. The features of the optical emission spectra obtained with the pulsed plasma source are significantly different from those obtained with direct current (dc) discharge higher power; for example, strong CH emission at 431.2 nm which is only weakly observed with dc plasma sources was observed, and the intense CN emission observed at 383-388 nm using dc plasma sources was weak in most cases. Strong CN emission was only observed using the present apparatus when compounds containing nitrogen, such as aniline were employed as samples. The present apparatus detects dimethylsulfoxide at 200 ppb using helium as the plasma gas by observing the emission band of the CH radical. When coupled with a gas chromatograph for separating components present in a sample to be analyzed, the present invention provides an apparatus for detecting the arrival of a particular component in the sample at the end of the chromatographic column and the identity thereof.

Alfvén Waves Generated by Expanding Plasmas in the Laboratory and in Space

NASA Astrophysics Data System (ADS)

Gekelman, W.; Vanzeeland, M.; Vincena, S.; Pribyl, P.

2002-12-01

There are many situations, which occur in space (coronal mass ejections, supernovas), or are man-made (upper atmospheric detonations) in which a dense plasma expands into a background magnetized plasma, that can support Alfvén waves. The LArge Plasma Device (LAPD) is a machine, at UCLA, in which Alfvén waves propagation in homogeneous and inhomogeneous plasmas has been studied. These will be briefly reviewed. Then a new class of experiments which involve the expansion of a dense (initially, n/no>>1) laser-produced plasma into an ambient highly magnetized background plasma capable of supporting Alfvén waves will be presented. The 150 MW laser is pulsed at the same 1 Hz repetition rate as the plasma in a highly reproducible experiment. The laser beam impacts a solid target such that the initial plasma burst is directed either along or across the magnetic field. The interaction results in the production of intense shear and compressional Alfvén waves, as well as large density perturbations. The waves propagate away from the target and are observed to become plasma column resonances. The magnetic fields of the waves are obtained with a 3-axis inductive probe. Spatial patterns of the magnetic fields associated with the waves and density perturbations are measured at over {10}4 locations and will be shown in dramatic movies. These are used to estimate the coupling efficiency of the laser energy and kinetic energy of the dense plasma into wave energy. The wave generation mechanism is due to field aligned return currents, which replace fast electrons escaping the initial blast. Work supported by ONR, DOE, and NSF

M = +1, ± 1 and ± 2 mode helicon wave excitation.

NASA Astrophysics Data System (ADS)

Kim, J.-H.; Yun, S.-M.; Chang, H.-Y.

1996-11-01

The characteristics of M=+1, ± 1 and ± 2 modes helicon wave excited using a solenoid antenna, Nagoya type III and quadrupole antenna respectively are first investigated. The solenoid antenna is constructed by winding a copper cable on a quartz discharge tube. Two dimensional cross-field measurements of ArII optical emission induced by hot electrons are made to investigate RF power deposition: Components of the wave magnetic field measured with a single-turn, coaxial magnetic probe were compared with the field patterns computed for M=+1, ± 1 and ± 2 modes. The M=+1 mode plasma produced by the solenoid antenna has a cylindrical high intensity plasma column, which center is empty. This cylindrical high intensity column results from the rotation of the cross-sectional electric field pattern (right hand circularly polarization). The radial plasma density profile has a peak at r=2.5cm with axisymmetry. It has been found that the radial profile of the plasma density is in good agreement with the computed power deposition profile. The radial profiles of the wave magnetic field are in good agreement with computations. The plasma excited by Nagoya type III antenna has two high intensity columns which results from the linear combination of M=+1 and -1 modes (i.e. plane polarization). The radial plasma density profile is in good agreement with emission intensity profile of ArII line (488nm). The plasma excited by quadrupole antenna has four high intensity columns which results from the linear combination of M=+2 and -2 modes (i.e. plane polarization). In the M=± 2 modes, the radial plasma density profile is also in good agreement with emission intensity profile of ArII line.

Characterizing the plasma of the Rotating Wall Machine

NASA Astrophysics Data System (ADS)

Hannum, David A.

The Rotating Wall Machine (RoWM) is a line-tied linear screw pinch built to study current-driven external kink modes. The plasma column is formed by an array of seven electrostatic washer guns which can also be biased to drive plasma current. The array allows independent control over the electron density ne and current density Jz profiles of the column. Internal measurements of the plasma have been made with singletip Langmuir and magnetic induction ("B-dot") probes for a range of bias currents (Ib = 0, 300, 500 A/gun). Streams from the individual guns are seen to merge at a distance of z ≈ 36 cm from the guns; the exact distance depends on the value of Ib. The density of the column is directly proportional to the Ohmic dissipation power, but the temperature stays at a low, uniform value (Te ≈ 3.5 eV) for each bias level. Electron densities are on the order of ne ˜10 20 m-3. The electron density expands radially (across the Bz guide field) as the plasma moves along the column, though the current density Jz mainly stays parallel to the field lines. The singletip Langmuir probe diagnostic is difficult to analyze for Ib = 500 A/gun plasmas and fails as Ib is raised beyond this level. Spectrographic analysis of the Halpha line indicates that the hydrogen plasmas are nearly fully ionized at each bias level. Azimuthal E x B rotation is axially and radially sheared; rotation slows as the plasma reaches the anode. Perpendicular diffusivity is consistent with the classical value, D⊥ ≈ 5 m2/sec, while parallel resistivity is seen to be twice the classical Spitzer value, 2 x 10-4 O m.

Use of a novel cation-exchange restricted-access material for automated sample clean-up prior to the determination of basic drugs in plasma by liquid chromatography.

PubMed

Chiap, P; Rbeida, O; Christiaens, B; Hubert, Ph; Lubda, D; Boos, K S; Crommen, J

2002-10-25

A new kind of silica-based restricted-access material (RAM) has been tested in pre-columns for the on-line solid-phase extraction (SPE) of basic drugs from directly injected plasma samples before their quantitative analysis by reversed-phase liquid chromatography (LC), using the column switching technique. The outer surface of the porous RAM particlescontains hydrophilic diol groups while sulphonic acid groups are bound to the internal surface, which gives the sorbent the properties of a strong cation exchanger towards low molecular mass compounds. Macromolecules such as proteins have no access to the internal surface of the pre-column due to their exclusion from the pores and are then flushed directly out. The retention capability of this novel packing material has been tested for some hydrophilic basic drugs, such as atropine, fenoterol, ipratropium, procaine, sotalol and terbutaline, used as model compounds. The influence of the composition of the washing liquid on the retention of the analytes in the pre-column has been investigated. The elution profiles of the different compounds and the plasma matrix as well as the time needed for the transfer of the analytes from the pre-column to the analytical column were determined in order to deduce the most suitable conditions for the clean-up step and develop on-line methods for the LC determination of these compounds in plasma. The cationic exchange sorbent was also compared to another RAM, namely RP-18 ADS (alkyl diol silica) sorbent with respect to retention capability towards basic analytes.

Semi-micro high-performance liquid chromatographic analysis of tiropramide in human plasma using column-switching.

PubMed

Baek, Soo Kyoung; Lee, Seung Seok; Park, Eun Jeon; Sohn, Dong Hwan; Lee, Hye Suk

2003-02-05

A rapid and sensitive column-switching semi-micro high-performance liquid chromatography method was developed for the direct analysis of tiropramide in human plasma. The plasma sample (100 microl) was directly injected onto Capcell Pak MF Ph-1 precolumn where deproteinization and analyte fractionation occurred. Tiropramide was then eluted into an enrichment column (Capcell Pak UG C(18)) using acetonitrile-potassium phosphate (pH 7.0, 50 mM) (12:88, v/v) and was analyzed on a semi-micro C(18) analytical column using acetonitrile-potassium phosphate (pH 7.0, 10 mM) (50:50, v/v). The method showed excellent sensitivity (limit of quantification 5 ng/ml), and good precision (C.V.

Double-pulse THz radiation bursts from laser-plasma acceleration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bosch, R. A.

2006-11-15

A model is presented for coherent THz radiation produced when an electron bunch undergoes laser-plasma acceleration and then exits axially from a plasma column. Radiation produced when the bunch is accelerated is superimposed with transition radiation from the bunch exiting the plasma. Computations give a double-pulse burst of radiation comparable to recent observations. The duration of each pulse very nearly equals the electron bunch length, while the time separation between pulses is proportional to the distance between the points where the bunch is accelerated and where it exits the plasma. The relative magnitude of the two pulses depends upon bymore » the radius of the plasma column. Thus, the radiation bursts may be useful in diagnosing the electron bunch length, the location of the bunch's acceleration, and the plasma radius.« less

[Purification of arsenic-binding proteins in hamster plasma after oral administration of arsenite].

PubMed

Wang, Wenwen; Zhang, Min; Li, Chunhui; Qin, Yingjie; Hua, Naranmandura

2013-01-01

To purify the arsenic-binding proteins (As-BP) in hamster plasma after a single oral administration of arsenite (iAs(III)). Arsenite was given to hamsters in a single dose. Three types of HPLC columns, size exclusion, gel filtration and anion exchange columns, combined with an inductively coupled argon plasma mass spectrometer (ICP MS) were used to purify the As-BP in hamster plasma. SDS-PAGE was used to confirm the arsenic-binding proteins at each purification step. The three-step purification process successfully separated As-BP from other proteins (ie, arsenic unbound proteins) in hamster plasma. The molecular mass of purified As-BP in plasma was approximately 40-50 kD on SDS-PAGE. The three-step purification method is a simple and fast approach to purify the As-BP in plasma samples.

Nonlinear MHD simulation of current drive by multi-pulsed coaxial helicity injection in spherical torus

NASA Astrophysics Data System (ADS)

Kanki, Takashi; Nagata, Masayoshi; Kagei, Yasuhiro

2011-10-01

The dynamics of structures of magnetic field, current density, and plasma flow generated during multi-pulsed coaxial helicity injection in spherical torus is investigated by 3-D nonlinear MHD simulations. During the driven phase, the flux and current amplifications occur due to the merging and magnetic reconnection between the preexisting plasma in the confinement region and the ejected plasma from the gun region involving the n = 1 helical kink distortion of the central open flux column (COFC). Interestingly, the diamagnetic poloidal flow which tends toward the gun region is then observed due to the steep pressure gradients of the COFC generated by ohmic heating through an injection current winding around the inboard field lines, resulting in the formation of the strong poloidal flow shear at the interface between the COFC and the core region. This result is consistent with the flow shear observed in the HIST. During the decay phase, the configuration approaches the axisymmetric MHD equilibrium state without flow because of the dissipation of magnetic fluctuation energy to increase the closed flux surfaces, suggesting the generation of ordered magnetic field structure. The parallel current density λ concentrated in the COFC then diffuses to the core region so as to reduce the gradient in λ, relaxing in the direction of the Taylor state.

Determination of N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine in human plasma by solid-phase extraction and column-switching high-performance liquid chromatography with ultraviolet detection.

PubMed

Ono, I; Matsuda, K; Kanno, S

1996-04-12

A column-switching high-performance liquid chromatography method with ultraviolet detection at 210 nm has been developed for the determination of N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine (AY4166, I) in human plasma. Plasma samples were prepared by solid-phase extraction with Sep-Pak Light tC18, followed by HPLC. The calibration graph for I was linear in the range 0.1-20 micrograms/ml. The limit of quantitation of I, in plasma, was 0.05 microgram/ml. The recovery of spiked I (0.5 microgram/ml) to drug-free plasma was over 92% and the relative standard deviation of spiked I (0.5 microgram/ml) compared to drug-free plasma was 4.3% (n = 8).

Observations of electron heating during 28 GHz microwave power application in proto-MPEX

DOE PAGES

Biewer, Theodore M.; Bigelow, Tim S.; Caneses Marin, Juan F.; ...

2018-02-01

The Prototype Material Plasma Exposure Experiment at the Oak Ridge National Laboratory utilizes a variety of power systems to generate and deliver a high heat flux plasma onto the surface of material targets. In the experiments described here, a deuterium plasma is produced via a ~100 kW, 13.56 MHz RF helicon source, to which ~20 kW of 28 GHz microwave power is applied. The electron density and temperature profiles are measured using a Thomson scattering (TS) diagnostic, and indicate that the electron density is centrally peaked. In the core of the plasma column, the electron density is higher than themore » cut-off density (~0.9 × 1019 m -3) for the launched mixture of X- and O-mode electron cyclotron heating waves to propagate. TS measurements indicate electron temperature increases from ~5 eV to ~20 eV during 28 GHz power application when the neutral deuterium pressure is reduced below 0.13 Pa (~1 mTorr.).« less

Observations of electron heating during 28 GHz microwave power application in proto-MPEX

NASA Astrophysics Data System (ADS)

Biewer, T. M.; Bigelow, T. S.; Caneses, J. F.; Diem, S. J.; Green, D. L.; Kafle, N.; Rapp, J.; Proto-MPEX Team

2018-02-01

The Prototype Material Plasma Exposure Experiment at the Oak Ridge National Laboratory utilizes a variety of power systems to generate and deliver a high heat flux plasma onto the surface of material targets. In the experiments described here, a deuterium plasma is produced via a ˜100 kW, 13.56 MHz RF helicon source, to which ˜20 kW of 28 GHz microwave power is applied. The electron density and temperature profiles are measured using a Thomson scattering (TS) diagnostic, and indicate that the electron density is centrally peaked. In the core of the plasma column, the electron density is higher than the cut-off density (˜0.9 × 1019 m-3) for the launched mixture of X- and O-mode electron cyclotron heating waves to propagate. TS measurements indicate electron temperature increases from ˜5 eV to ˜20 eV during 28 GHz power application when the neutral deuterium pressure is reduced below 0.13 Pa (˜1 mTorr.).

Simulations of drift-Alfven turbulence in LAPD using BOUT

NASA Astrophysics Data System (ADS)

Popovich, Pavel; Umansky, Maxim; Carter, Troy; Cowley, Steve

2008-11-01

The LArge Plasma Device (LAPD) at UCLA is a 17 m long, 60 cm diameter magnetized plasma column with typical plasma parameters ne˜1x10^12cm-3, Te˜10eV, and B ˜1kG. The simple geometry and extensive measurement capability on LAPD allows for detailed comparison with and validation of numerical simulations of turbulence and transport. We analyse the LAPD results using simulations with the boundary plasma turbulence code BOUT. BOUT models the 3D electromagnetic plasma turbulence solving a system of fluid moment equations in a general tokamak geometry near the boundary. We will discuss the physical model and modifications of the BOUT code required for the LAPD configuration, and present the first results of the simulations and comparison to experimental measurements. In particular, a confinement transition is observed in LAPD under the application of bias-driven rotation. Also, intermittent generation and convection of filamentary structures (``blobs'' and ``holes'') is observed in the LAPD edge. Application of BOUT to modeling of these two phenomena will be discussed. E. Maggs, T.A. Carter, and R.J. Taylor, Phys. Plasmas 14, (2007) T.A. Carter, Phys. Plasmas 13, (2006)

Investigation of beam- and wave-plasma interactions in spherical tokamak Globus-M

NASA Astrophysics Data System (ADS)

Gusev, V. K.; Aminov, R. M.; Berezutskiy, A. A.; Bulanin, V. V.; Chernyshev, F. V.; Chugunov, I. N.; Dech, A. V.; Dyachenko, V. V.; Ivanov, A. E.; Khitrov, S. A.; Khromov, N. A.; Kurskiev, G. S.; Larionov, M. M.; Melnik, A. D.; Minaev, V. B.; Mineev, A. B.; Mironov, M. I.; Miroshnikov, I. V.; Mukhin, E. E.; Novokhatsky, A. N.; Panasenkov, A. A.; Patrov, M. I.; Petrov, A. V.; Petrov, Yu. V.; Podushnikova, K. A.; Rozhansky, V. A.; Rozhdestvensky, V. V.; Sakharov, N. V.; Shevelev, A. E.; Senichenkov, I. Yu.; Shcherbinin, O. N.; Stepanov, A. Yu.; Tolstyakov, S. Yu.; Varfolomeev, V. I.; Voronin, A. V.; Yagnov, V. A.; Yashin, A. Yu.; Zhilin, E. G.

2011-10-01

The experimental and theoretical results obtained in the last two years on the interaction of neutral particle beams and high-frequency waves with a plasma using the spherical tokamak Globus-M are discussed. The experiments on the injection of low-energy proton beam of ~300 eV directed particle energy are performed with a plasma gun that produces a hydrogen plasma jet of density up to 3 × 1022 m-3 and a high velocity up to 250 km s-1. A moderate density rise (up to 30%) is achieved in the central plasma region without plasma disruption. Experiments on high-energy (up to 30 keV) neutral beam injection into the D-plasma are analysed. Modelling results on confinement of fast particles inside the plasma column that follows the neutral beam injection are discussed. The influence of the magnetic field on the fast particle losses is argued. A neutral beam injection regime with primary ion heating is obtained and discussed. The new regime with fast current ramp-up and early neutral beam injection shows electron temperature rise and formation of broad Te profiles until the q = 1 flux surface enters the plasma column. An energetic particle mode in the range of frequencies 5-30 kHz and toroidal Alfvén eigenmodes in the range 50-300 kHz are recorded in that regime simultaneously with the Te rise. The energetic particle mode and toroidal Alfvén eigenmodes behaviour are discussed. The toroidal Alfvén eigenmode spectrum appears in Globus-M as a narrow band corresponding to n = 1. The first experimental results on plasma start-up and noninductive current drive generation are presented. The experiments are carried out with antennae providing mostly poloidal slowing down of waves with a frequency of 920 MHz, which is higher than a lower hybrid one existing under the experimental conditions. The high current drive efficiency is shown to be high (of about 0.25 A W-1), and its mechanism is proposed. Some near future plans of the experiments are also discussed.

Observation of helicon wave with m = 0 antenna in a weakly magnetized inductively coupled plasma source

NASA Astrophysics Data System (ADS)

Ellingboe, Bert; Sirse, Nishant; Moloney, Rachel; McCarthy, John

2015-09-01

Bounded whistler wave, called ``helicon wave,'' is known to produce high-density plasmas and has been exploited as a high density plasma source for many applications, including electric propulsion for spacecraft. In a helicon plasma source, an antenna wrapped around the magnetized plasma column launches a low frequency wave, ωce/2 >ωhelicon >ωce/100, in the plasma which is responsible for maintaining high density plasma. Several antenna designs have been proposed in order to match efficiently the wave modes. In our experiment, helicon wave mode is observed using an m = 0 antenna. A floating B dot probe, compensated to the capacitively coupled E field, is employed to measure axial-wave-field-profiles (z, r, and θ components) in the plasma at multiple radial positions as a function of rf power and pressure. The Bθ component of the rf-field is observed to be unaffected as the wave propagates in the axial direction. Power coupling between the antenna and the plasma column is identified and agrees with the E, H, and wave coupling regimes previously seen in M =1 antenna systems. That is, the Bz component of the rf-field is observed at low plasma density as the Bz component from the antenna penetrates the plasma. The Bz component becomes very small at medium density due to shielding at the centre of the plasma column; however, with increasing density, a sudden ``jump'' occurs in the Bz component above which a standing wave under the antenna with a propagating wave away from the antenna are observed.

Transformation of the ordered internal structures during the acceleration of fast charged particles in a dense plasma focus

NASA Astrophysics Data System (ADS)

Kubes, P.; Paduch, M.; Cikhardt, J.; Cikhardtova, B.; Klir, D.; Kravarik, J.; Rezac, K.; Zielinska, E.; Sadowski, M. J.; Szymaszek, A.; Tomaszewski, K.; Zaloga, D.

2017-07-01

The paper concerns important differences in the evolution of plasma column structures during the production of fusion neutrons in the first and subsequent neutron pulses, as observed for plasma-focus discharges performed with the deuterium filling. The first neutron pulse, of a more isotropic distribution, is usually produced during the formation of the first big plasmoid. The next neutron pulses can be generated by the fast deuterons moving dominantly in the downstream direction, at the instants of a disruption of the pinch constriction, when other plasmoids are formed during the constriction evolution. In both cases, the fusion neutrons are produced by a beam-target mechanism, and the acceleration of fast electron- and deuteron-beams can be interpreted by transformation and decay of the magnetic field associated with a filamentary structure of the current flow in the plasmoid.

Determination of N-(trans-4-isopropylcyclohexanecarbonyl)-D-phenylalanine and its metabolites in human plasma and urine by column-switching high-performance liquid chromatography with ultraviolet detection.

PubMed

Ono, I; Matsuda, K; Kanno, S

1997-05-09

A simple, rapid and sensitive two column-switching high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 210 nm has been developed for the determination of N-(trans-4-isopropylcyclohexanecarbonyl)-D-phenylalanine (AY4166, I) and its seven metabolites in human plasma and urine. Measurements of I and its metabolites were carried out by two column-switching HPLC, because metabolites were classified into two groups according to their retention times. After purification of plasma samples using solid-phase extraction and direct dilution of urinary samples, I and each metabolite were injected into HPLC. The calibration graphs for plasma and urinary samples were linear in the ranges 0.1 to 10 microg ml(-1) and 0.5 to 50 microg ml(-1), respectively. Recoveries of I and its seven metabolites were over 88% by the standard addition method and the relative standard deviations of I and its metabolites were 1-6%.

High-quality electron beams from beam-driven plasma accelerators by wakefield-induced ionization injection.

PubMed

Martinez de la Ossa, A; Grebenyuk, J; Mehrling, T; Schaper, L; Osterhoff, J

2013-12-13

We propose a new and simple strategy for controlled ionization-induced trapping of electrons in a beam-driven plasma accelerator. The presented method directly exploits electric wakefields to ionize electrons from a dopant gas and capture them into a well-defined volume of the accelerating and focusing wake phase, leading to high-quality witness bunches. This injection principle is explained by example of three-dimensional particle-in-cell calculations using the code OSIRIS. In these simulations a high-current-density electron-beam driver excites plasma waves in the blowout regime inside a fully ionized hydrogen plasma of density 5×10(17)cm-3. Within an embedded 100  μm long plasma column contaminated with neutral helium gas, the wakefields trigger ionization, trapping of a defined fraction of the released electrons, and subsequent acceleration. The hereby generated electron beam features a 1.5 kA peak current, 1.5  μm transverse normalized emittance, an uncorrelated energy spread of 0.3% on a GeV-energy scale, and few femtosecond bunch length.

Microscale depletion of high abundance proteins in human biofluids using IgY14 immunoaffinity resin: analysis of human plasma and cerebrospinal fluid.

PubMed

Hyung, Seok-Won; Piehowski, Paul D; Moore, Ronald J; Orton, Daniel J; Schepmoes, Athena A; Clauss, Therese R; Chu, Rosalie K; Fillmore, Thomas L; Brewer, Heather; Liu, Tao; Zhao, Rui; Smith, Richard D

2014-11-01

Removal of highly abundant proteins in plasma is often carried out using immunoaffinity depletion to extend the dynamic range of measurements to lower abundance species. While commercial depletion columns are available for this purpose, they generally are not applicable to limited sample quantities (<20 μL) due to low yields stemming from losses caused by nonspecific binding to the column matrix and concentration of large eluent volumes. Additionally, the cost of the depletion media can be prohibitive for larger-scale studies. Modern LC-MS instrumentation provides the sensitivity necessary to scale-down depletion methods with minimal sacrifice to proteome coverage, which makes smaller volume depletion columns desirable for maximizing sample recovery when samples are limited, as well as for reducing the expense of large-scale studies. We characterized the performance of a 346 μL column volume microscale depletion system, using four different flow rates to determine the most effective depletion conditions for ∼6-μL injections of human plasma proteins and then evaluated depletion reproducibility at the optimum flow rate condition. Depletion of plasma using a commercial 10-mL depletion column served as the control. Results showed depletion efficiency of the microscale column increased as flow rate decreased, and that our microdepletion was reproducible. In an initial application, a 600-μL sample of human cerebrospinal fluid (CSF) pooled from multiple sclerosis patients was depleted and then analyzed using reversed phase liquid chromatography-mass spectrometry to demonstrate the utility of the system for this important biofluid where sample quantities are more commonly limited.

Microscale depletion of high abundance proteins in human biofluids using IgY14 immunoaffinity resin: Analysis of human plasma and cerebrospinal fluid

DOE PAGES

Hyung, Seok Won; Piehowski, Paul D.; Moore, Ronald J.; ...

2014-09-06

Removal of highly abundant proteins in plasma is often carried out using immunoaffinity depletion to extend the dynamic range of measurements to lower abundance species. While commercial depletion columns are available for this purpose, they generally are not applicable to limited sample quantities (<20 µL) due to low yields stemming from losses caused by nonspecific binding to the column matrix. Additionally, the cost of the depletion media can be prohibitive for larger scale studies. Modern LC-MS instrumentation provides the sensitivity necessary to scale-down depletion methods with minimal sacrifice to proteome coverage, which makes smaller volume depletion columns desirable for maximizingmore » sample recovery when samples are limited, as well as for reducing the expense of large scale studies. We characterized the performance of a 346 µL column volume micro-scale depletion system, using four different flow rates to determine the most effective depletion conditions for ~6 μL injections of human plasma proteins and then evaluated depletion reproducibility at the optimum flow rate condition. Depletion of plasma using a commercial 10 mL depletion column served as the control. Results showed depletion efficiency of the micro-scale column increased as flow rate decreased, and that our micro-depletion was reproducible. We found, in an initial application, a 600 µL sample of human cerebral spinal fluid (CSF) pooled from multiple sclerosis patients was depleted and then analyzed using reversed phase liquid chromatography-mass spectrometry to demonstrate the utility of the system for this important biofluid where sample quantities are more commonly limited.« less

Pattern formation and filamentation in low temperature, magnetized plasmas - a numerical approach

NASA Astrophysics Data System (ADS)

Menati, Mohamad; Konopka, Uwe; Thomas, Edward

2017-10-01

In low-temperature discharges under the influence of high magnetic field, pattern and filament formation in the plasma has been reported by different groups. The phenomena present themselves as bright plasma columns (filaments) oriented parallel to the magnetic field lines at high magnetic field regime. The plasma structure can filament into different shapes from single columns to spiral and bright rings when viewed from the top. In spite of the extensive experimental observations, the observed effects lack a detailed theoretical and numerical description. In an attempt to numerically explain the plasma filamentation, we present a simplified model for the plasma discharge and power deposition into the plasma. Based on the model, 2-D and 3-D codes are being developed that solve Poisson's equation along with the fluid equations to obtain a self-consistent description of the plasma. The model and preliminary results applied to the specific plasma conditions will be presented. This work was supported by the US Dept. of Energy and NSF, DE-SC0016330, PHY-1613087.

Stereospecific analysis of loxoprofen in plasma by chiral column liquid chromatography with a circular dichroism-based detector.

PubMed

Kanazawa, Hideko; Tsubayashi, Akane; Nagata, Yoshiko; Matsushima, Yoshikazu; Mori, Chiharu; Kizu, Junko; Higaki, Megumu

2002-03-01

The chiral separation of loxoprofen was achieved on a chiral column with UV and circular dichroism (CD) detection. The good resolution of four loxoprofen stereoisomers was obtained. The column used for the chiral separation was Chiralcel OJ column (250 x 4.6 mm) using hexane-2-propanol-trifluoroacetic acid (95:5:0.1), as an eluent. The flow-rate was 1.0 ml/min and the detection was at 225 nm. In addition, CD and UV spectra were obtained by stopped flow scanning. The method allows the determination of the stereoisomers of loxoprofen in human plasma after the administration of therapeutic dose of the racemic drug, thus HPLC with CD detector is useful for the stereospecific determination of loxoprofen products in biological samples.

Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis.

PubMed

Vasdev, Neil; Collier, Thomas Lee

2016-08-17

Column-switching high performance liquid chromatography (HPLC) is extensively used for the critical analysis of radiolabeled ligands and their metabolites in plasma. However, the lack of streamlined apparatus and consequently varying protocols remain as a challenge among positron emission tomography laboratories. We report here the prototype apparatus and implementation of a fully automated and simplified column-switching procedure to allow for the easy and automated determination of radioligands and their metabolites in up to 5 mL of plasma. The system has been used with conventional UV and coincidence radiation detectors, as well as with a single quadrupole mass spectrometer.

Particle-in-cell simulations of bounded plasma discharges applied to low pressure high density sources and positive columns

NASA Astrophysics Data System (ADS)

Kawamura, Emi

Particle-in-cell (PIC) simulations of bounded plasma discharges are attractive because the fields and the particle motion can be obtained self-consistently from first principles. Thus, we can accurately model a wide range of nonlocal and kinetic behavior. The only disadvantage is that PIC may be computationally expensive compared to other methods. Fluid codes, for example, may run faster but make assumptions about the bulk plasma velocity distributions and ignore kinetic effects. In Chapter 1, we demonstrate methods of accelerating PIC simulations of bounded plasma discharges. We find that a combination of physical and numerical methods makes run-times for PIC codes much more competitive with other types of codes. In processing plasmas, the ion energy distributions (IEDs) arriving at the wafer target are crucial in determining ion anisotropy and etch rates. The current trend for plasma reactors is towards lower gas pressure and higher plasma density. In Chapter 2, we review and analyze IEDs arriving at the target of low pressure high density rf plasma reactors. In these reactors, the sheath is typically collisionless. We then perform PIC simulations of collisionless rf sheaths and find that the key parameter governing the shape of the TED at the wafer is the ratio of the ion transit time across the sheath over the rf period. Positive columns are the source of illumination in fluorescent mercury-argon lamps. The efficiency of light production increases with decreasing gas pressure and decreasing discharge radius. Most current lamp software is based on the local concept even though low pressure lighting discharges tend to be nonlocal. In Chapter 3, we demonstrate a 1d3v radial PIC model to conduct nonlocal kinetic simulations of low pressure, small radius positive columns. When compared to other available codes, we find that our PIC code makes the least approximations and assumptions and is accurate and stable over a wider parameter range. We analyze the PIC simulation results in detail and find that the radial electron heat flow, which is neglected in local models, plays a major role in maintaining the global power balance. In Chapter 2, we focused on the sheaths of low pressure high density plasma reactors. In Chapter 4, we extend our study to the bulk and presheaths. Typical industrial plasma reactors often use gases with complex chemistries which tend to generate discharges containing negative ions. For high density electronegative plasmas with low gas pressure, we expect Coulomb collisions between positive and negative ions to dominate over collisions between ions and neutrals. We incorporate a Coulomb collision model into our PIC code to study the effect of this ion-ion Coulomb scattering. We find that the Coulomb collisions between the positive and negative ions significantly modify the negative ion flux, density and kinetic energy profiles.

Electromagnetic Wave Transmittance Control using Anisotropic Plasma Lattice

NASA Astrophysics Data System (ADS)

Matlis, Eric; Corke, Thomas; Hoffman, Anthony

2017-11-01

Experiments of transmission through a lattice array of plasma columns have shown an absorption band close to the plasma frequency at 14 GHz. The beam was oriented at a 35° incident angle to the planar plasma cell. These experiments were designed to determine if the observed absorption was the result of the isotropic plasma medium or that of an anisotropic metamaterial. Transmission of the microwave energy was not consistent with an isotropic material in which absorption would monotonically increase below the plasma frequency. The experimental results are supported by an anisotropic model which was developed for the plasma permittivity using an effective medium approximation. The plasma columns were modeled as uniform rods with permittivity described by a Drude model while the components of the permittivity tensor was calculated using the Maxwell-Garnett effective medium theory. Electron densities of n = 4 x1012 cm-3 were assumed which is consistent with prior experimental measurements. This model confirms the existence of non-zero imaginary wave vector k in a narrow region centered about 14 GHz.

Model of vertical plasma motion during the current quench

NASA Astrophysics Data System (ADS)

Breizman, Boris; Kiramov, Dmitrii

2017-10-01

Tokamak disruptions impair plasma position control, which allows the plasma column to move and hit the wall. These detrimental events enhance thermal and mechanical loads due to halo currents and runaway electron losses. Their fundamental understanding and prevention is one of the high-priority items for ITER. As commonly observed in experiments, the disruptive plasma tends to move vertically, and the timescale of this motion is rather resistive than Alfvenic. These observations suggest that the plasma column is nearly force-free during its vertical motion. In fact, the force-free constraint is already used in disruption simulators. In this work, we consider a geometrically simple system that mimics the tokamak plasma surrounded by the conducting structures. Using this model, we highlight the underlying mechanism of the vertical displacement events during the current quench phase of plasma disruption. We also address a question of ideal MHD stability of the plasma during its resistive motion. Work supported by the U.S. Department of Energy Contracts DEFG02-04ER54742 and DE-SC0016283.

Analysis of carvedilol enantiomers in human plasma using chiral stationary phase column and liquid chromatography with tandem mass spectrometry.

PubMed

Poggi, Josiane Cristófani; Da Silva, Flávia Garcez; Coelho, Eduardo Barbosa; Marques, Maria Paula; Bertucci, Carlo; Lanchote, Vera Lucia

2012-03-01

Carvedilol is an antihypertensive drug available as a racemic mixture. (-)-(S)-carvedilol is responsible for the nonselective β-blocker activity but both enantiomers present similar activity on α(1)-adrenergic receptor. To our knowledge, this is the first study of carvedilol enantiomers in human plasma using a chiral stationary phase column and liquid chromatography with tandem mass spectrometry. The method involves plasma extraction with diisopropyl ether using metoprolol as internal standard and direct separation of the carvedilol enantiomers on a Chirobiotic T® (Teicoplanin) column. Protonated ions [M + H](+) and their respective ion products were monitored at transitions of 407 > 100 for the carvedilol enantiomers and 268 > 116 for the internal standard. The quantification limit was 0.2 ng ml(-1) for both enantiomers in plasma. The method was applied to study enantioselectivity in the pharmacokinetics of carvedilol administered as a single dose of 25 mg to a hypertensive patient. The results showed a higher plasma concentration of (+)-(R)-carvedilol (AUC(0-∞) 205.52 vs. 82.61 (ng h) ml(-1)), with an enantiomer ratio of 2.48. Copyright © 2012 Wiley Periodicals, Inc.

Method for making a non-extractable stationary phase of polymer within a capillary column

DOEpatents

Springston, Stephen R.

1990-01-01

A method for coating interior capillary column surfaces, or packing material of a packed column, used for gas chromatography, with a stationary polymer phase that is cross-linked by exposing it to a low-temperature plasma that is uniformly distributed over the column or packing material for a predetermined period of time to effect the desired degree of cross-linking of the coating.

Rapid quantification of underivatized amino acids in plasma by hydrophilic interaction liquid chromatography (HILIC) coupled with tandem mass-spectrometry.

PubMed

Prinsen, Hubertus C M T; Schiebergen-Bronkhorst, B G M; Roeleveld, M W; Jans, J J M; de Sain-van der Velden, M G M; Visser, G; van Hasselt, P M; Verhoeven-Duif, N M

2016-09-01

Amino acidopathies are a class of inborn errors of metabolism (IEM) that can be diagnosed by analysis of amino acids (AA) in plasma. Current strategies for AA analysis include cation exchange HPLC with post-column ninhydrin derivatization, GC-MS, and LC-MS/MS-related methods. Major drawbacks of the current methods are time-consuming procedures, derivative problems, problems with retention, and MS-sensitivity. The use of hydrophilic interaction liquid chromatography (HILIC) columns is an ideal separation mode for hydrophilic compounds like AA. Here we report a HILIC-method for analysis of 36 underivatized AA in plasma to detect defects in AA metabolism that overcomes the major drawbacks of other methods. A rapid, sensitive, and specific method was developed for the analysis of AA in plasma without derivatization using HILIC coupled with tandem mass-spectrometry (Xevo TQ, Waters). Excellent separation of 36 AA (24 quantitative/12 qualitative) in plasma was achieved on an Acquity BEH Amide column (2.1×100 mm, 1.7 μm) in a single MS run of 18 min. Plasma of patients with a known IEM in AA metabolism was analyzed and all patients were correctly identified. The reported method analyzes 36 AA in plasma within 18 min and provides baseline separation of isomeric AA such as leucine and isoleucine. No separation was obtained for isoleucine and allo-isoleucine. The method is applicable to study defects in AA metabolism in plasma.

Effects of Coulomb collisions on cyclotron maser and plasma wave growth in magnetic loops

NASA Technical Reports Server (NTRS)

Hamilton, Russell J.; Petrosian, Vahe

1990-01-01

The evolution of nonthermal electrons accelerated in magnetic loops is determined by solving the kinetic equation, including magnetic field convergence and Coulomb collisions in order to determine the effects of these interactions on the induced cyclotron maser and plasma wave growth. It is found that the growth rates are larger and the possibility of cyclotron maser action is stronger for smaller loop column density, for larger magnetic field convergence, for a more isotropic injected electron pitch angle distribution, and for more impulsive acceleration. For modest values of the column density in the coronal portion of a flaring loop, the growth rates of instabilities are significantly reduced, and the reduction is much larger for the cyclotron modes than for the plasma wave modes. The rapid decrease in the growth rates with increasing loop column density suggests that, in flare loops when such phenomena occur, the densities are lower than commonly accepted.

Beam-driven acceleration in ultra-dense plasma media

DOE PAGES

Shin, Young-Min

2014-09-15

Accelerating parameters of beam-driven wakefield acceleration in an extremely dense plasma column has been analyzed with the dynamic framed particle-in-cell plasma simulator, and compared with analytic calculations. In the model, a witness beam undergoes a TeV/m scale alternating potential gradient excited by a micro-bunched drive beam in a 10 25 m -3 and 1.6 x 10 28 m -3 plasma column. The acceleration gradient, energy gain, and transformer ratio have been extensively studied in quasi-linear, linear-, and blowout-regimes. The simulation analysis indicated that in the beam-driven acceleration system a hollow plasma channel offers 20 % higher acceleration gradient by enlargingmore » the channel radius (r) from 0.2 Ap to 0.6 .Ap in a blowout regime. This paper suggests a feasibility of TeV/m scale acceleration with a hollow crystalline structure (e.g. nanotubes) of high electron plasma density.« less

Rarefaction waves, solitons, and holes in a pure electron plasma

NASA Astrophysics Data System (ADS)

Moody, J. D.; Driscoll, C. F.

1995-12-01

The propagation of holes, solitons, and rarefaction waves along the axis of a magnetized pure electron plasma column is described. The time dependence of the radially averaged density perturbation produced by the nonlinear waves is measured at several locations along the plasma column for a wide range of plasma parameters. The rarefaction waves are studied by measuring the free expansion of the plasma into a vacuum. A new hydrodynamic theory is described that quantitatively predicts the free expansion measurements. The rarefaction is initially characterized by a self-similar plasma flow, resulting in a perturbed density and velocity without a characteristic length scale. The electron solitons show a small increase in propagation speed with increasing amplitude and exhibit electron bursts. The holes show a decrease in propagation speed with increasing amplitude. Collisions between holes and solitons show that these objects pass through each other undisturbed, except for a small offset.

Determination of alpidem, an imidazopyridine anxiolytic, and its metabolites by column-switching high-performance liquid chromatography with fluorescence detection.

PubMed

Flaminio, L; Ripamonti, M; Ascalone, V

1994-05-13

Alpidem, 6-chloro-2-(4-chlorophenyl)-N,N-dipropylimidazo[1,2-a]pyridine- 3-acetamide, is an anxiolytic imidazopyridine that undergoes a first-pass elimination after oral administration to humans; it is actively metabolized and three circulating metabolites have been identified in plasma due to N-dealkylation, oxidation or a combination of both processes. For the determination of the unchanged drug and its metabolites in human plasma, a column-switching HPLC method was developed. The method, based on solid-phase extraction (performed on-line), involves the automatic injection of plasma samples (200 microliters) on to a precolumn filled with C18 material, clean-up of the sample with water in order to remove protein and salts and transfer of the analytes to the analytical column (after valve switching) by means of the mobile phase. All the processes were performed in the presence of an internal standard, a compound chemically related to alpidem. During the analytical chromatography, the precolumn was flushed with different solvents and after regeneration with water, it was ready for further injections. The analytical column was a C8 type and the mobile phase was acetonitrile-methanol-phosphate buffer solution (45:15:45, v/v/v) at a flow-rate of 1.5 ml min-1. The column was connected to a fluorimetric detector operating at excitation and emission wavelengths of 255 and 423 nm, respectively. The limits of quantitation of alpidem and three metabolites were 2.5 and 1.5 ng ml-1, respectively, in human plasma.

Effects of Hot Limiter Biasing on Tokamak Runaway Discharges

NASA Astrophysics Data System (ADS)

Salar Elahi, A.; Ghoranneviss, M.; Ghanbari, M. R.

2013-10-01

In this research hot limiter biasing effects on the Runaway discharges were investigated. First wall of the tokamak reactors can affects serious damage due to the high energy runaway electrons during a major disruption and therefore its life time can be reduced. Therefore, it is important to find methods to decrease runaway electron generation and their energy. Tokamak limiter biasing is one of the methods for controlling the radial electric field and can induce a transition to an improved confinement state. In this article generation of runaway electrons and the energy they can obtain will be investigated theoretically. Moreover, in order to apply radial biasing an emissive limiter biasing is utilized. The biased limiter can apply +380 V in the status of cold and hot to the plasma and result in the increase of negative bias current in hot status. In fact, in this experiment we try to decrease the generation of runaway electrons and their energy by using emissive limiter biasing inserted on the IR-T1 tokamak. The mean energy of these electrons was obtained by spectroscopy of hard X-ray. Also, the plasma current center shift was measured from the vertical field coil characteristics in presence of limiter biasing. The calculation is made focusing on the vertical field coil current and voltage changes due to a horizontal displacement of plasma column.

Characteristics of X-Ray Emission from the PFRC-2 Capacitively Coupled Plasma

NASA Astrophysics Data System (ADS)

Oliver, Richard; Pearcy, Jacob; Jandovitz, Peter; Swanson, Charles; Matteucci, Jackson; Cohen, Samuel; PFRC Team

2015-11-01

It is uncertain what causes keV X-rays emitted from the central-cell region of a cool (bulk Te ~ 4 eV), tenuous (ne ~1010 cm-3), 5 cm diameter, weakly ionized hydrogen plasma column generated in a tandem high-mirror-ratio mirror machine (PFRC-2 device) by a low-power, external, capacitively-coupled RF (27 MHz) antenna. We explored whether the energetic electrons responsible for the X-rays exist only in the central cell (ER) or also in the asymmetric mirror regions at opposite ends of the machine, as well as how the spectra compare if they do exist in both regions. To address this, we have designed, built, calibrated, installed and operated an X-ray detector system to view the PFRC-2 region near the RF antenna in one end cell (MC). We observe somewhat different X-ray spectra emanating from the two regions. The system comprises two Amptek XR-100CR detectors with moveable slits that scan across the plasma column. Further control of radial resolution (to 0.4 cm) is afforded by changing the detector-to-slit distance. Calibrations were performed with an 55Fe source. These data are being used to understand the source of the fast electrons that create the X-rays in the MC and in the ER. This work is supported by the US DOE Contract No. DE-AC02-09CH11466 and the Princeton Environmental Institute.

Method for making a non-extractable stationary phase of polymer within a capillary column

DOEpatents

Springston, S.R.

1990-10-30

A method is described for coating interior capillary column surfaces, or packing material of a packed column, used for gas chromatography, with a stationary polymer phase that is cross-linked by exposing it to a low-temperature plasma that is uniformly distributed over the column or packing material for a predetermined period of time to effect the desired degree of cross-linking of the coating. 7 figs.

Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

DOEpatents

Knapp, Jr., Furn F.; Lisic, Edward C.; Mirzadeh, Saed; Callahan, Alvin P.

1993-01-01

A generator system for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form.

Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

DOEpatents

Knapp, F.F. Jr.; Lisic, E.C.; Mirzadeh, S.; Callahan, A.P.

1993-02-16

A generator system for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form.

Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

DOEpatents

Knapp, Jr., Furn F.; Lisic, Edward C.; Mirzadeh, Saed; Callahan, Alvin P.

1994-01-01

A generator system for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form.

Synthesis of the flavonoid 3',4',5'-trimethoxyflavonol and its determination in plasma and tissues of mice by HPLC with fluorescence detection.

PubMed

Britton, Robert G; Fong, Isabel; Saad, Shaban; Brown, Karen; Steward, William P; Gescher, Andreas; Sale, Stewart

2009-04-01

3',4',5'-Trimethoxyflavonol (TMFol) was synthesized as a potential colorectal cancer chemopreventive agent. An HPLC method for determination for TMFol in murine plasma and tissues was developed and validated using human plasma. Analyte was separated (C(18) column; fluorescence detection 330nm excitation, 440nm emission) using 69% methanol and 0.1M ammonium acetate buffer (pH 5.1) as mobile phase. The method was linear for 50-2500ng/ml plasma and 0.05-10microg/g tissue (r>0.99). TMFol was recovered from plasma or tissues using solid phase columns or organic solvent protein precipitation, respectively. Recovery at low, medium and high concentrations was 97.6-107.3%, with inter- and intra-day coefficients of variation of <10%. The lower limit of quantitation for plasma was 50ng/ml. The method was applied to measure steady-state TMFol plasma and tissue levels in mice which received dietary TMFol (0.2%).

Interaction of Fast Ions with Global Plasma Modes in the C-2 Field Reversed Configuration Experiment

NASA Astrophysics Data System (ADS)

Smirnov, Artem; Dettrick, Sean; Clary, Ryan; Korepanov, Sergey; Thompson, Matthew; Trask, Erik; Tuszewski, Michel

2012-10-01

A high-confinement operating regime [1] with plasma lifetimes significantly exceeding past empirical scaling laws was recently obtained by combining plasma gun edge biasing and tangential Neutral Beam Injection (NBI) in the C-2 field-reversed configuration (FRC) experiment [2, 3]. We present experimental and computational results on the interaction of fast ions with the n=2 rotational and n=1 wobble modes in the C-2 FRC. It is found that the n=2 mode is similar to quadrupole magnetic fields in its detrimental effect on the fast ion transport due to symmetry breaking. The plasma gun generates an inward radial electric field, thus stabilizing the n=2 rotational instability without applying the quadrupole magnetic fields. The resultant FRCs are nearly axisymmetric, which enables fast ion confinement. The NBI further suppresses the n=2 mode, improves the plasma confinement characteristics, and increases the plasma configuration lifetime [4]. The n=1 wobble mode has relatively little effect on the fast ion transport, likely due to the approximate axisymmetry about the displaced plasma column. [4pt] [1] M. Tuszewski et al., Phys. Rev. Lett. 108, 255008 (2012).[0pt] [2] M. Binderbauer et al., Phys. Rev. Lett. 105, 045003 (2010).[0pt] [3] H.Y. Guo et al., Phys. Plasmas 18, 056110 (2011).[0pt] [4] M. Tuszewski et al., Phys. Plasmas 19, 056108 (2012)

Characteristics of a DC-Driven Atmospheric Pressure Air Microplasma Jet

NASA Astrophysics Data System (ADS)

Choi, Jaegu; Matsuo, Keita; Yoshida, Hidekazu; Namihira, Takao; Katsuki, Sunao; Akiyama, Hidenori

2008-08-01

A dc-driven atmospheric pressure air plasma jet has been investigated for some applications, such as local dental treatment, the inner surface treatment of capillaries, stimuli for microorganisms, and the local cleaning of semiconductor devices. The main experimental results are as follows. The discharge in the pulsed mode occurs repetitively despite of the dc input, and the pulsed mode transfers to the continuous mode as the current exceeds a threshold. The measured emission spectrum from the arc column of the air discharge reveals that most energy of activated electrons is used for the excitation of N2 (second positive system bands) and part of the energy for the dissociation of O2. The length of the plasma torch depends on the tube length, inner gap distance, and flow rate. The maximum torch length of about 40 mm is obtained under certain conditions. The spatial distributions of plasma gas temperature are measured and confirmed by the visualization of the gas flow using Schlieren images. Furthermore, surface treatment and decolorization using the generated plasma torch are carried out, focusing on industrial applications.

Modeling and Theory of RF Antenna Systems on Proto-MPEX

NASA Astrophysics Data System (ADS)

Piotrowicz, P. A.; Caneses, J. F.; Goulding, R. H.; Green, D.; Caughman, J. B. O.; Ruzic, D. N.; Proto-MPEX Team

2017-10-01

The RF wave coupling of the helicon and ICH antennas installed on the Prototype Material Plasma Exposure eXperiment (MPEX) has been explored theoretically and via a full wave model implemented in COMSOL Multiphysics. The high-density mode in Proto-MPEX has been shown to occur when exciting radial eigenmodes of the plasma column which coincides with entering a Trivelpiece Gould (TG) anti-resonant regime, therefore suppressing edge heating in favor of core power deposition. The fast wave launched by the helicon antenna has a large wavelength and travels at a steep group velocity angle with the background magnetic field; for this reason the fast wave launched by the helicon antenna efficiently couples power to the core plasma. However, the ICH heating scheme relies on a small wavelength slow wave to couple power to the core of the plasma column. Coupling slow wave power to the core of the plasma column is sensitive to the location of the Alfven resonance. The wave-vector and group velocity vector of the slow wave in this parameter regime undergoes a drastic change in behavior when approaching the Alfven resonance. Full wave simulation results and dispersion analysis will be presented with suggestions to guide experimental progress. This work was supported by the US. D.O.E. contract DE-AC05-00OR22725.

Nonmonotonic radial distribution of excited atoms in a positive column of pulsed direct currect discharges in helium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barnat, E. V.; Kolobov, V. I.

2013-01-21

Nonmonotonic radial distributions of excited helium atoms have been experimentally observed in a positive column of pulsed helium discharges using planar laser induced fluorescence. Computational analysis of the discharge dynamics with a fluid plasma model confirms the experimental observations over a range of pressures and currents. The observed effect is attributed to the peculiarities of electron population-depopulation of the excited states during the 'dynamic discharge' conditions with strong modulations of the electric field maintaining the plasma.

Cylindrical stationary striations in surface wave produced plasma columns of argon

NASA Astrophysics Data System (ADS)

Kumar, Rajneesh; Kulkarni, Sanjay V.; Bora, Dhiraj

2007-12-01

Striations are a good example of manifestation of a glow discharge. In the present investigation, stationary striations in the surface wave produced plasma column are formed. Physical parameters (length, number, etc.) of such striations can be controlled by operating parameters. With the help of bifurcation theory, experimental results are explained by considering two-step ionization in the surface wave discharge mechanism in argon gas. It is also observed that the bifurcation parameter is a function of input power, working pressure, and tube radius.

Tungsten-188/carrier-free rhenium-188 perrhenic acid generator system

DOEpatents

Knapp, F.F. Jr.; Lisic, E.C.; Mirzadeh, S.; Callahan, A.P.

1994-01-04

A generator system has been invented for providing a carrier-free radioisotope in the form of an acid comprises a chromatography column in tandem fluid connection with an ion exchange column, the chromatography column containing a charge of a radioactive parent isotope. The chromatography column, charged with a parent isotope, is eluted with an alkali metal salt solution to generate the radioisotope in the form of an intermediate solution, which is passed through the ion-exchange column to convert the radioisotope to a carrier-free acid form. 1 figure.

Electric discharge during electrosurgery

PubMed Central

Shashurin, Alexey; Scott, David; Zhuang, Taisen; Canady, Jerome; Beilis, Isak I.; Keidar, Michael

2015-01-01

Electric discharge utilized for electrosurgery is studied by means of a recently developed method for the diagnostics of small-size atmospheric plasma objects based on Rayleigh scattering of microwaves on the plasma volume. Evolution of the plasma parameters in the near-electrode sheaths and in the positive column is measured and analyzed. It is found that the electrosurgical system produces a glow discharge of alternating current with strongly contracted positive column with current densities reaching 103 A/cm2. The plasma electron density and electrical conductivities in the channel were found be 1016 cm−3 and (1-2) Ohm−1cm−1, respectively. The discharge interrupts every instance when the discharge-driving AC voltage crosses zero and re-ignites again every next half-wave at the moment when the instant voltage exceeds the breakdown threshold. PMID:25880721

Electric discharge during electrosurgery.

PubMed

Shashurin, Alexey; Scott, David; Zhuang, Taisen; Canady, Jerome; Beilis, Isak I; Keidar, Michael

2015-04-16

Electric discharge utilized for electrosurgery is studied by means of a recently developed method for the diagnostics of small-size atmospheric plasma objects based on Rayleigh scattering of microwaves on the plasma volume. Evolution of the plasma parameters in the near-electrode sheaths and in the positive column is measured and analyzed. It is found that the electrosurgical system produces a glow discharge of alternating current with strongly contracted positive column with current densities reaching 10(3) A/cm(2). The plasma electron density and electrical conductivities in the channel were found be 10(16) cm(-3) and (1-2) Ohm(-1) cm(-1), respectively. The discharge interrupts every instance when the discharge-driving AC voltage crosses zero and re-ignites again every next half-wave at the moment when the instant voltage exceeds the breakdown threshold.

High-performance liquid chromatographic determination of the beta2-selective adrenergic agonist fenoterol in human plasma after fluorescence derivatization.

PubMed

Kramer, S; Blaschke, G

2001-02-10

A sensitive high-performance liquid chromatographic method has been developed for the determination of the beta2-selective adrenergic agonist fenoterol in human plasma. To improve the sensitivity of the method, fenoterol was derivatized with N-(chloroformyl)-carbazole prior to HPLC analysis yielding highly fluorescent derivatives. The assay involves protein precipitation with acetonitrile, liquid-liquid-extraction of fenoterol from plasma with isobutanol under alkaline conditions followed by derivatization with N-(chloroformyl)-carbazole. Reversed-phase liquid chromatographic determination of the fenoterol derivative was performed using a column-switching system consisting of a LiChrospher 100 RP 18 and a LiChrospher RP-Select B column with acetonitrile, methanol and water as mobile phase. The limit of quantitation in human plasma was 376 pg fenoterol/ml. The method was successfully applied for the assay of fenoterol in patient plasma.

Determination of highly protein bound drugs in plasma using high-performance liquid chromatography and column switching, exemplified by the retinoids.

PubMed

Wyss, R; Bucheli, F

1988-12-02

During method development for the determination of either isotretinoin, tretinoin and their 4-oxo-metabolites, or etretinate, acitretin and 13-cis-acitretin in plasma using high-performance liquid chromatography and column switching, recovery problems arose, when undiluted plasma samples were injected directly onto the precolumn. These recovery problems may be due to the strong binding of the retinoids to different plasma proteins. Measures to overcome this strong protein binding, such as variation of the injection solution composition and the purge mobile phase, were systematically investigated. Best recoveries were obtained by diluting of plasma with 9 mM sodium hydroxide-acetonitrile (8:2, v/v) and protein precipitation with ethanol for the isotretinoin and etretinate series, respectively, in combination with the use of a purge mobile phase containing ammonium acetate and 10-20% acetonitrile. Less effective was the use of a longer precolumn or heating of the precolumn.

Mode conversion and heating in a UCLA-high schools collaborative experiment

NASA Astrophysics Data System (ADS)

Smith, Miana; Buckley-Bonnano, Samuel; Pribyl, Patrick; Gekelman, Walter; Wise, Joe; Baker, Bob; Marmie, Ken

2016-10-01

A small plasma device is in operation for use by undergraduates and high school students at UCLA. Magnetic field up to 100 G, with density 108 <=ne <=1011cm-3 and temperature Te < 3eV are available in a 50 cm diameter plasma 2 meters long. The plasma is generated by an ICP source at one end operating at about 500 kHz. For this experiment, a small plate located near the edge of the plasma column is used as an electrostatic launcher. High frequency waves ωce < ω < 3ωce are launched radially from the plate in the low-density region, with electric field perpendicular to B and to the density gradient. A Langmuir probe located some distance away axially measures plasma heating along a field line that passes several cm in front of the launcher, localized in radius with δr 1cm Absorption and strong electron heating are observed at the plasma resonant layer. We explore the ``double resonance condition at which ωpe = 2ωce . Here strong interaction with electron Bernstein waves is expected. The Bernstein waves are also launched at low power and their dispersion relation verified. Work done at the BaPSF at UCLA which is supported by the DOE/NSF.

Eigenmode electric field profiles in cylindrical plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Litwin, C.; Hershkowitz, N.

Electric field profiles of plasma column eigenmodes in the ion-cyclotron range of frequencies are discussed. Step and parabolic density profiles are compared. The role of temperature and Alfven resonance is analyzed.

Equilibrium fitting analysis and propagation of magnetic fluctuations in the Multi-pulsing HIST plasmas

NASA Astrophysics Data System (ADS)

Matsumoto, K.; Hanano, T.; Ito, K.; Ishihara, M.; Higashi, T.; Kikuchi, Y.; Fukumoto, N.; Nagata, M.

2011-10-01

The current drive by Multi-pulsing Coaxial Helicity Injection (M-CHI) has been performed on HIST in a wide range of configurations from high-q ST to low-q ST and spheromak generated by the utilization of the toroidal field. It is a key issue to investigate the dynamo mechanism required to maintain each configuration. To identify the detail mechanisms regarding a helicity transport from the edge to the core region, we have investigated the characteristics of magnetic field fluctuations observed in M- CHI experiments. We have fitted internal magnetic field data to a ST configuration calculated by the equilibrium code with a hollow pressure profile in order to find the sustained configurations. Fluctuation frequency is identified as about 80 kHz and it has been found to propagate from the open flux column region toward the core region. The toroidal mode n=0 is dominant in the high TF coil current operation. Alfven wave generation has been identified by evaluating its velocity as a function of plasma density or magnetic field strength. We will discuss the relationship between the Alfven wave and helicity propagation.

Simultaneous determination of acetylsalicylic acid and salicylic acid in human plasma by isocratic high-pressure liquid chromatography with post-column hydrolysis and fluorescence detection.

PubMed

Hobl, Eva-Luise; Jilma, Bernd; Ebner, Josef; Schmid, Rainer W

2013-06-01

A selective, sensitive and rapid high-performance liquid chromatography method with post-column hydrolysis and fluorescence detection was developed for the simultaneous quantification of acetylsalicylic acid and its metabolite salicylic acid in human plasma. Following the addition of 2-hydroxy-3-methoxybenzoic acid as internal standard and simple protein precipitation with acetonitrile, the analytes were separated on a ProntoSIL 120 C18 ace-EPS column (150 × 2 mm, 3 µm) protected by a C8 guard column (5 µm). The mobile phase, 10 mm formic acid in water (pH 2.9) and acetonitrile (70:30, v/v), was used at a flow rate of 0.35 mL/min. After on-line post-column hydrolysis of acetylsalicylic acid (ASA) to salicylic acid (SA) by addition of alkaline solution, the analytes were measured at 290 nm (λex ) and 400 nm (λem ). The method was linear in the concentration ranges between 0.05 and 20 ng/μL for both ASA and SA with a lower limit of quantification of 25 pg/μL for SA and 50 pg/μL for ASA. The limit of detection was 15 pg/μL for SA and 32.5 pg/μL for ASA. The analysis of ASA and SA can be carried out within 8 min; therefore this method is suitable for measuring plasma concentrations of salicylates in clinical routine. Copyright © 2012 John Wiley & Sons, Ltd.

Polymyxin B-immobilized fiber column hemoperfusion removes endotoxin throughout a 24-hour treatment period.

PubMed

Mitaka, Chieko; Fujiwara, Naoto; Yamamoto, Mamoru; Toyofuku, Takahiro; Haraguchi, Go; Tomita, Makoto

2014-10-01

The purpose of this study was to evaluate the extent of endotoxin adsorption by polymyxin B-immobilized fiber column hemoperfusion (PMX) performed for a 24-hour treatment period in patients with septic shock. Nineteen patients with septic shock were retrospectively studied. The plasma endotoxin concentrations of blood drawn from the radial artery and from the outlet circuit of the PMX column were measured by kinetic turbidimetric limulus assay using an MT-358 Toxinometer (Wako Pure Chemical Industries, Ltd, Osaka, Japan) after 24 hours of PMX treatment. The endotoxin removal rate was defined by the following equation: ([radial artery endotoxin concentration - outlet circuit of PMX column endotoxin concentration]/radial artery endotoxin concentration) × 100%. The patients had a median Acute Physiology and Chronic Health Evaluation II score of 29 at intensive care unit admission and a 28-day mortality of 47%. Before the start of the PMX treatment, the median radial arterial plasma endotoxin concentration was 16.48 pg/mL. After 24 hours of PMX treatment, the median radial plasma endotoxin concentration had decreased to 1.857 pg/mL, and the concentration at the outlet circuit of the PMX column was further decreased to 0.779 pg/mL. The median endotoxin removal rate was 74.4%. These findings suggest that 24-hour PMX treatment was effective in removing endotoxin continuously throughout the entire treatment period. Copyright © 2014 Elsevier Inc. All rights reserved.

Flow and dynamo measurements during the coaxial helicity injection on HIST

NASA Astrophysics Data System (ADS)

Ando, K.; Higashi, T.; Nakatsuka, M.; Kikuchi, Y.; Fukumoto, N.; Nagata, M.

2009-11-01

The current drive by Coaxial Helicity Injection (CHI-CD) was performed on HIST in a wide range of configurations from high-q ST to low-q ST and spheromak generated by the utilization of the toroidal field. It is a key issue to investigate the dynamo mechanism required to maintain each configuration. To identify the detail mechanisms, it is needed to manifest a role of plasma flows in the CHI-CD. For this purpose, we have measured the ion flow and the dynamo electric field using an ion Doppler spectrometer (IDS) system, a Mach probe and a dynamo probe. The new dynamo probe consists of 3-axis Mach probes and magnetic pick-up coils. The flow measurements have shown that the intermittent generation of the flow is correlated to the fluctuation seen on the electron density and current signals during the driven phase. At this time, the toroidal direction of the ion flow in the central open flux column is opposite to that of the toroidal current there, i.e. the same direction as electrons. After the plasma enters to the resistive decay phase, the toroidal flow tends to reverse to the same direction as the toroidal current. The results are consistent with the model of the repetitive plasmoid ejection and coalescence proposed for CHI-CD. The plasma jet emanating from the gun source and magnetic field generations through reconnection during the driven phase is well reflected in the 3D MHD simulation.

Study of Plasma Chemistry and Plasma Processing.

DTIC Science & Technology

1983-01-01

m, 20% 0,0-oxybisfpropionitrile] on Chrom W column in these reactions comes from their pertinence to prebiotic was used for cyanogen and gaseous...xerography, prebiotic chemistry, and chemistry in the ionosphere. L -5- Euipment for RF Reactions Virtually all of the work on organic plasmas has used RF

Validated hydrophilic interaction LC-MS/MS method for simultaneous quantification of dacarbazine and 5-amino-4-imidazole-carboxamide in human plasma.

PubMed

Liu, Yanhong; Zhang, Weihua; Yang, Yuhui

2008-10-19

A hydrophilic interaction high performance liquid chromatography-tandem mass spectrometric method has been developed and validated for simultaneous quantification of dacarbazine (DTIC) and its terminal metabolite, 5-amino-4-imidazole-carboxamide (AIC) in human plasma. The plasma samples are first extracted by a C8+SCX mixed-mode 96-well plate to extend the extraction stability of DTIC and AIC. The extracted residues are further cleaned by a primary and secondary amine (PSA) adsorbent for minimization of matrix effect. Analyses are done on an Amide-80 HPLC column coupled to a tandem mass spectrometer fitted with an atmospheric pressure turbo ion spray ionization interface in the positive-ion mode. Both DTIC and AIC have reproducible retention times on the Amide-80 HPLC column. This type of column not only has an excellent column life (over 4000 injections), but also has zero carryover effect. The injection volume should be limited at 10 microL or less to avoid the peak splitting. The validated concentration ranges are from 0.5 to 500 ng/mL for DTIC and from 2.0 to 500 ng/mL for AIC. The validated method has been successfully applied to determine the pharmacokinetic profiles for human patients receiving DTIC infusions.

Gas arc constriction for plasma arc welding

NASA Technical Reports Server (NTRS)

McGee, William F. (Inventor); Rybicki, Daniel J. (Inventor)

1994-01-01

A welding torch for plasma arc welding apparatus has an inert gas applied circumferentially about the arc column externally of the constricting nozzle so as to apply a constricting force on the arc after it has exited the nozzle orifice and downstream of the auxiliary shielding gas. The constricting inert gas is supplied to a plenum chamber about the body of the torch and exits through a series of circumferentially disposed orifices in an annular wall forming a closure at the forward end of the constricting gas plenum chamber. The constricting force of the circumferential gas flow about the arc concentrates and focuses the arc column into a more narrow and dense column of energy after exiting the nozzle orifice so that the arc better retains its energy density prior to contacting the workpiece.

Plasma flow measurements in the Prototype-Material Plasma Exposure eXperiment (Proto-MPEX) and comparison with B2.5-Eirene modeling

NASA Astrophysics Data System (ADS)

Kafle, N.; Owen, L. W.; Caneses, J. F.; Biewer, T. M.; Caughman, J. B. O.; Donovan, D. C.; Goulding, R. H.; Rapp, J.

2018-05-01

The Prototype Material Plasma Exposure eXperiment (Proto-MPEX) at Oak Ridge National Laboratory is a linear plasma device that combines a helicon plasma source with additional microwave and radio frequency heating to deliver high plasma heat and particle fluxes to a target. Double Langmuir probes and Thomson scattering are being used to measure local electron temperature and density at various radial and axial locations. A recently constructed Mach-double probe provides the added capability of simultaneously measuring electron temperatures ( T e), electron densities ( n e), and Mach numbers (M). With this diagnostic, it is possible to infer the plasma flow, particle flux, and heat flux at different locations along the plasma column in Proto-MPEX. Preliminary results show Mach numbers of 0.5 (towards the dump plate) and 1.0 (towards the target plate) downstream from the helicon source, and a stagnation point (no flow) near the source for the case where the peak magnetic field was 1.3 T. Measurements of particle flow and ne and Te profiles are discussed. The extensive coverage provided by these diagnostics permits data-constrained B2.5-Eirene modeling of the entire plasma column, and comparison with results of modeling in the high-density helicon plasmas will be presented.

The MaPLE device of Saha Institute of Nuclear Physics: construction and its plasma aspects.

PubMed

Pal, Rabindranath; Biswas, Subir; Basu, Subhasis; Chattopadhyay, Monobir; Basu, Debjyoti; Chaudhuri, Manis; Chowdhuri, Manis

2010-07-01

The Magnetized Plasma Linear Experimental (MaPLE) device is a low cost laboratory plasma device at Saha Institute of Nuclear Physics fabricated in-house with the primary aim of studying basic plasma physics phenomena such as plasma instabilities, wave propagation, and their nonlinear behavior in magnetized plasma regime in a controlled manner. The machine is specially designed to be a versatile laboratory device that can provide a number of magnetic and electric scenario to facilitate such studies. A total of 36 number of 20-turn magnet coils, designed such as to allow easy handling, is capable of producing a uniform, dc magnetic field of about 0.35 T inside the plasma chamber of diameter 0.30 m. Support structure of the coils is planned in an innovative way facilitating straightforward fabrication and easy positioning of the coils. Further special feature lies in the arrangement of the spacers between the coils that can be maneuvered rather easily to create different magnetic configurations. Various methods of plasma production can be suitably utilized according to the experimental needs at either end of the vacuum vessel. In the present paper, characteristics of a steady state plasma generated by electron cyclotron resonance method using 2.45 GHz microwave power are presented. Scans using simple probe drives revealed that a uniform and long plasma column having electron density approximately 3-5x10(10) cm(-3) and temperature approximately 7-10 eV, is formed in the center of the plasma chamber which is suitable for wave launching experiments.

Increasing Plasma Parameters using Sheared Flow Stabilization of a Z-Pinch

NASA Astrophysics Data System (ADS)

Shumlak, Uri

2016-10-01

Recent experiments on the ZaP Flow Z-Pinch at the University of Washington have been successful in compressing the plasma column to smaller radii, producing the predicted increases in plasma density (1018 cm-3), temperature (200 eV), and magnetic fields (4 T), while maintaining plasma stability for many Alfven times (over 40 μs) using sheared plasma flows. These results indicate the suitability of the device as a discovery science platform for astrophysical and high energy density plasma research, and keeps open a possible path to achieving burning plasma conditions in a compact fusion device. Long-lived Z-pinch plasmas have been produced with dimensions of 1 cm radius and 100 cm long that are stabilized by sheared axial flows for over 1000 Alfven radial transit times. The observed plasma stability is coincident with the presence of a sheared flow as measured by time-resolved multi-chord ion Doppler spectroscopy applied to impurity ion radiation. These measurements yield insights into the evolution of the velocity profile and show that the stabilizing behavior of flow shear agrees with theoretical calculations and 2-D MHD computational simulations. The flow shear value, extent, and duration are shown to be consistent with theoretical models of the plasma viscosity, which places a design constraint on the maximum axial length of a sheared flow stabilized Z-pinch. Measurements of the magnetic field topology indicate simultaneous azimuthal symmetry and axial uniformity along the entire 100 cm length of the Z-pinch plasma. Separate control of plasma acceleration and compression have increased the accessible plasma parameters and have generated stable plasmas with radii below 0.5 cm, as measured with a high resolution digital holographic interferometer. This work was supported by Grants from U.S. DOE, NNSA, and ARPA-E.

Calculation of extracted ion beam particle distribution including within-extractor collisions from H-alpha Doppler shift measurements.

PubMed

Kim, Tae-Seong; Kim, Jinchoon; In, Sang Ryul; Jeong, Seung Ho

2008-02-01

Prototype long pulse ion sources are being developed and tested toward the goal of a deuterium beam extraction of 120 keV/65 A. The latest prototype source consists of a magnetic bucket plasma generator and a four-grid copper accelerator system with multicircular apertures of 568 holes. To measure the angular divergence and the ion species of the ion beam, an optical multichannel analyzer (OMA) system for a Doppler-shifted H-alpha lights was set up at the end of a gas-cell neutralizer. But the OMA data are very difficult to analyze due to a large background level on the top of the three energy peaks (coming from H(+), H(2) (+), and H(3) (+)). These background spectra in the OMA signals seem to result from partially accelerated ion beams in the accelerator. Extracted ions could undergo a premature charge exchange as the accelerator column tends to have a high hydrogen partial pressure from the unused gas from the plasma generator, resulting in a continuous background of partially accelerated beam particles at the accelerator exit. This effect is calculated by accounting for all the possible atomic collision processes and numerically summing up three ion species across the accelerator column. The collection of all the atomic reaction cross sections and the numerical summing up will be presented. The result considerably depends on the background pressure and the ion beam species ratio (H(+), H(2) (+), and H(3) (+)). This effect constitutes more than 20% of the whole particle distribution. And the energy distribution of those suffering from collisions is broad and shows a broad maximum in the vicinity of the half and the third energy region.

Compact electron beam focusing column

NASA Astrophysics Data System (ADS)

Persaud, Arun; Leung, Ka-Ngo; Reijonen, Jani

2001-12-01

A novel design for an electron beam focusing column has been developed at LBNL. The design is based on a low-energy spread multicusp plasma source which is used as a cathode for electron beam production. The focusing column is 10 mm in length. The electron beam is focused by means of electrostatic fields. The column is designed for a maximum voltage of 50 kV. Simulations of the electron trajectories have been performed by using the 2D simulation code IGUN and EGUN. The electron temperature has also been incorporated into the simulations. The electron beam simulations, column design and fabrication will be discussed in this presentation.

Role of electric fields in the MHD evolution of the kink instability

DOE PAGES

Lapenta, Giovanni; Skender, Marina

2017-02-17

Here, the discovery of electrostatic fields playing a crucial role in establishing plasma motion in the flux conversion and dynamo processes in reversed field pinches is revisited. In order to further elucidate the role of the electrostatic fields, a flux rope configuration susceptible to the kink instability is numerically studied with anMHDcode. Simulated nonlinear evolution of the kink instability is found to confirm the crucial role of the electrostatic fields. Anew insight is gained on the special function of the electrostatic fields: they lead the plasma towards the reconnection site at the mode resonant surface. Without this step the plasmamore » column could not relax to its nonlinear state, since no other agent is present to perform this role. While the inductive field generated directly by the kink instability is the dominant flow driver, the electrostatic field is found to allow the motion in the vicinity of the reconnection region.« less

Role of electric fields in the MHD evolution of the kink instability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lapenta, Giovanni; Skender, Marina

Here, the discovery of electrostatic fields playing a crucial role in establishing plasma motion in the flux conversion and dynamo processes in reversed field pinches is revisited. In order to further elucidate the role of the electrostatic fields, a flux rope configuration susceptible to the kink instability is numerically studied with anMHDcode. Simulated nonlinear evolution of the kink instability is found to confirm the crucial role of the electrostatic fields. Anew insight is gained on the special function of the electrostatic fields: they lead the plasma towards the reconnection site at the mode resonant surface. Without this step the plasmamore » column could not relax to its nonlinear state, since no other agent is present to perform this role. While the inductive field generated directly by the kink instability is the dominant flow driver, the electrostatic field is found to allow the motion in the vicinity of the reconnection region.« less

Determination of plasma azathioprine and 6-mercaptopurine in patients with rheumatoid arthritis treated with oral azathioprine.

PubMed

Liliemark, J; Pettersson, B; Lafolie, P; Zweig, T; Peterson, C

1990-07-01

Two specific high-performance liquid chromatography methods for determining plasma concentrations of azathioprine and 6-mercaptopurine after oral administration of azathioprine are presented. It was shown that azathioprine is unstable in the blood samples unless immediately cooled in ice water. The 2-amino analog, guaneran, was used as internal standard for azathioprine, which was extracted from plasma with ethylacetate. A Nucleosil C18 column was used for the separation. The detection limit was 6 nM. For quantification of 6-mercaptopurine, 6-thioguanine was used as internal standard. Plasma was deproteinized with HClO4 and the sample was purified on mercurial cellulose. A Beckman ODS column was used and the detection limit was 5 nM. Pharmacokinetic data from two patients are presented. Unchanged azathioprine was seen until 6 h after an oral dose of 32 mg/m2.

Positron production by x rays emitted by betatron motion in a plasma wiggler.

PubMed

Johnson, D K; Auerbach, D; Blumenfeld, I; Barnes, C D; Clayton, C E; Decker, F J; Deng, S; Emma, P; Hogan, M J; Huang, C; Ischebeck, R; Iverson, R; Joshi, C; Katsouleas, T C; Kirby, N; Krejcik, P; Lu, W; Marsh, K A; Mori, W B; Muggli, P; O'Connell, C L; Oz, E; Siemann, R H; Walz, D; Zhou, M

2006-10-27

Positrons in the energy range of 3-30 MeV, produced by x rays emitted by betatron motion in a plasma wiggler of 28.5 GeV electrons from the SLAC accelerator, have been measured. The extremely high-strength plasma wiggler is an ion column induced by the electron beam as it propagates through and ionizes dense lithium vapor. X rays in the range of 1-50 MeV in a forward cone angle of 0.1 mrad collide with a 1.7 mm thick tungsten target to produce electron-positron pairs. The positron spectra are found to be strongly influenced by the plasma density and length as well as the electron bunch length. By characterizing the beam propagation in the ion column these influences are quantified and result in excellent agreement between the measured and calculated positron spectra.

Some Research Centers for Plasma Physics and Solid State Physics in the Netherlands and Belgium. Part II. Belgium,

DTIC Science & Technology

plasma column and observed the interesting phenomenon of plasma ejection. At FUB, Balescu and Prigogine direct a group of sixty theoreticians doing...outstanding work in statistical physics. Balescu is writing another graduate textbook on non-equilibrium statistical mechanics. He is tackling the

Characterization of Flow and Ohm's Law in the Rotating Wall Machine

NASA Astrophysics Data System (ADS)

Hannum, David; Brookhart, M.; Forest, C. B.; Kendrick, R.; Mengin, G.; Paz-Soldan, C.

2010-11-01

The rotating wall machine is a linear screw-pinch built to study the role of different electromagnetic boundary conditions on the Resistive Wall Mode (RWM). Its plasma is created by an array of electrostatic washer guns which can be biased to discharge up to 1 kA of current each. Individual flux ropes from the guns shear, merge, and expand into a 20 cm diameter, ˜1 m long plasma column. Langmuir (singletip) and tri-axial B-dot probes move throughout the column to measure radial and axial profiles of key plasma parameters. As the plasma current increases, more H2 fuel is ionized, raising ne to 5 x10^20 m-3 while Te stays at a constant 3 eV. The electron density expands to the wall while the current density (Jz) stays pinched to the central axis. E xB and diamagnetic drifts create radially and axially sheared plasma rotation. Plasma resistivity follows the Spitzer model in the core while exceeding it at the edge. These measurements improve the model used to predict the RWM growth rate.

Experimental Results of OH Regime Investigation in Globus-M Spherical Torus

NASA Astrophysics Data System (ADS)

Golant, Victor; Gusev, Vasily; Levin, Roman; Petrov, Yuriy; Sakharov, Nikolay

2001-10-01

Plasma parameters were measured in novel spherical torus Globus-M in highly shaped plasmas with aspect ratio, A > 1.5, elongation, k < 1.9, triangularity < 0.5. Plasma column was created by direct induction method with the currents up to Ip 0.3 MA in the magnetic field, Bt - 0.08 - 0.5 T. In Globus-M spherical torus plasma column is closely fitted into the vacuum vessel and wall conditioning technology described in [1] was used to achieve good plasma performance. Plasma experiments were focused around achievement of ultimate OH regimes allowed by power supplies. The operational limits of the device were investigated. In the regime with extreme low q(cy1) < 1 and high normalized current > 4, the plasma current of almost 100kA was sustained transiently in low magnetic field 800 Gs. The first results on stability analysis with numerical code are presented. The runaway electrons behavior was studied in spherical tokamak conditions. Influence of plasma current and density ramp-up speeds, MHD events on plasma performance and stability was demonstrated. Magnetic reconstruction was performed with EFIT version adopted for PC simulations. Plans for auxiliary heating and current drive are discussed. 1. V.K. Gusev, …, V.E. Golant, et al., Nucl. Fusion 41, No 7, (2001), to be published

Development of an on-column enrichment technique based on C18-functionalized magnetic silica nanoparticles for the determination of lidocaine in rat plasma by high performance liquid chromatography.

PubMed

Chu, Bin; Lou, Dujuan; Yu, Panfeng; Hu, Shaonan; Shen, Shun

2011-10-14

In this study, a novel on-column enrichment technique filled with C(18)-functionalized magnetic silica nanoparticles was successfully developed for the determination of lidocaine in rat plasma by high performance liquid chromatography (HPLC). The synthesized Fe(3)O(4)@SiO(2)-C(18) nanoparticles were locally packed into the capillary by the application of magnets. Lidocaine in the sample solutions pumped into the capillary tube could be easily adsorbed by Fe(3)O(4)@SiO(2)-C(18) through hydrophobic interaction by the interior C(18) groups, and eluted by acetonitrile solution. Different extraction conditions were investigated. Method validations including linear range, quantification limit, detection limit, precision, accuracy and recovery were also studied. The results showed that the proposed method based on on-column enrichment by Fe(3)O(4)@SiO(2)-C(18) was a novel, little solvent and efficient approach for the determination of lidocaine in the complex plasma samples. Copyright © 2011 Elsevier B.V. All rights reserved.

Two-dimensional ion chromatography for the separation of ionic organophosphates generated in thermally decomposed lithium hexafluorophosphate-based lithium ion battery electrolytes.

PubMed

Kraft, Vadim; Grützke, Martin; Weber, Waldemar; Menzel, Jennifer; Wiemers-Meyer, Simon; Winter, Martin; Nowak, Sascha

2015-08-28

A two-dimensional ion chromatography (IC/IC) technique with heart-cutting mode for the separation of ionic organophosphates was developed. These analytes are generated during thermal degradation of three different commercially available Selectilyte™ lithium ion battery electrolytes. The composition of the investigated electrolytes is based on 1M lithium hexafluorophosphate (LiPF6) dissolved in ethylene carbonate/dimethyl carbonate (50:50wt%, LP30), ethylene carbonate/diethyl carbonate (50:50wt%, LP40) and ethylene carbonate/ethyl methyl carbonate (50:50wt%, LP50). The organophosphates were pre-separated from PF6(-) anion on the low capacity A Supp 4 column, which was eluted with a gradient step containing acetonitrile. The fraction containing analytes was retarded on a pre-concentration column and after that transferred to the high capacity columns, where the separation was performed isocratically. Different stationary phases and eluents were applied on the 2nd dimension for the investigation of retention times, whereas the highly promising results were obtained with a high capacitive A Supp 10 column. The organophosphates generated in LP30 and LP40 electrolytes could be separated by application of an aqueous NaOH eluent providing fast analysis time within 35min. For the separation of the organophosphates of LP50 electrolyte due to its complexity a NaOH eluent containing a mixture of methanol/H2O was necessary. In addition, the developed two dimensional IC method was hyphenated to an inductively coupled plasma mass spectrometer (ICP-MS) using aqueous NaOH without organic modifiers. This proof of principle measurement was carried out for future quantitative investigation regarding the concentration of the ionic organophosphates. Furthermore, the chemical stability of several ionic organophosphates in water and acetonitrile at room temperature over a period of 10h was investigated. In both solvents no decomposition of the investigated analytes was observed and therefore water as solvent for dilution of samples was proved as suitable. Copyright © 2015 Elsevier B.V. All rights reserved.

Fatigue and stress studies : an improved semi-automated procedure for flurometric determination of plasma catecholamines.

DOT National Transportation Integrated Search

1966-04-01

A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautom...

Determination of endogenous levels of 13-cis-retinoic acid (isotretinoin), all-trans-retinoic acid (tretinoin) and their 4-oxo metabolites in human and animal plasma by high-performance liquid chromatography with automated column switching and ultraviolet detection.

PubMed

Wyss, R; Bucheli, F

1997-10-24

A highly sensitive HPLC method with automated column switching was developed for the simultaneous determination of endogenous levels of 13-cis-retinoic acid (isotretinoin), all-trans-retinoic acid (tretinoin) and their 4-oxo metabolites in plasma samples from man, Cynomolgus monkey, rabbit, rat and mouse. Plasma (0.4 ml) was deproteinated by adding ethanol (1.5 ml) containing the internal standard acitretin. After centrifugation, 1.4 ml of the supernatant were directly injected onto the precolumn packed with LiChrospher 100 RP-18 (5 microm). 1.25% ammonium acetate and acetic acid-ethanol (8:2, v/v) was used as mobile phase during injection and 1% ammonium acetate and 2% acetic acid-ethanol (102:4, v/v) was added, on-line, to decrease the elution strength of the injection solution. After backflush purging of the precolumn, the retained components were transferred to the analytical column in the backflush mode, separated by gradient elution and detected at 360 nm. Two coupled Superspher 100 RP-18 endcapped columns (both 250x4 mm) were used for the separation, together with a mobile phase consisting of acetonitrile-water-10% ammonium acetate-acetic acid: (A) 600:300:60:10 (v/v/v/v), (B) 950:20:5:20 (v/v/v/v), and (C) 990:5:0:5 (v/v/v/v). The method was linear in the range 0.3-100 ng/ml, at least, with a quantification limit of 0.3 ng/ml. The mean recoveries from human plasma were 93.2%-94.4% and the mean inter-assay precision was 2.8%-3.2% (range 0.3-100 ng/ml). Similar results were obtained for animal plasma. The analytes were found to be stable in the plasma of all investigated species stored at -20 degrees C for 4.3 months and at -80 degrees C for 9 months, at least. At this temperature, human plasma samples were even stable for 2 years. The method was successfully applied to more than 6000 human and 1000 animal plasma samples from clinical and toxicokinetic studies. Endogenous levels determined in control patients and pregnant women were similar to published data from volunteers.

Concentration of perrhenate and pertechnetate solutions

DOEpatents

Knapp, F.F.; Beets, A.L.; Mirzadeh, S.; Guhlke, S.

1998-03-17

A method is described for preparing a concentrated solution of a carrier-free radioisotope which includes the steps of: (a) providing a generator column loaded with a composition containing a parent radioisotope; (b) eluting the generator column with an eluent solution which includes a salt of a weak acid to elute a target daughter radioisotope from the generator column in a first eluate; (c) eluting a cation-exchange column with the first eluate to exchange cations of the salt for hydrogen ions and to elute the target daughter radioisotope and a weak acid in a second eluate; (d) eluting an anion-exchange column with the second eluate to trap and concentrate the target daughter radioisotope and to elute the weak acid solution therefrom; and (e) eluting the concentrated target daughter radioisotope from the anion-exchange column with a saline solution. 1 fig.

Concentration of perrhenate and pertechnetate solutions

DOEpatents

Knapp, Furn F.; Beets, Arnold L.; Mirzadeh, Saed; Guhlke, Stefan

1998-01-01

A method of preparing a concentrated solution of a carrier-free radioisotope which includes the steps of: a. providing a generator column loaded with a composition containing a parent radioisotope; b. eluting the generator column with an eluent solution which includes a salt of a weak acid to elute a target daughter radioisotope from the generator column in a first eluate. c. eluting a cation-exchange column with the first eluate to exchange cations of the salt for hydrogen ions and to elute the target daughter radioisotope and a weak acid in a second eluate; d. eluting an anion-exchange column with the second eluate to trap and concentrate the target daughter radioisotope and to elute the weak acid solution therefrom; and e. eluting the concentrated target daughter radioisotope from the anion-exchange column with a saline solution.

Novel non-equilibrium modelling of a DC electric arc in argon

NASA Astrophysics Data System (ADS)

Baeva, M.; Benilov, M. S.; Almeida, N. A.; Uhrlandt, D.

2016-06-01

A novel non-equilibrium model has been developed to describe the interplay of heat and mass transfer and electric and magnetic fields in a DC electric arc. A complete diffusion treatment of particle fluxes, a generalized form of Ohm’s law, and numerical matching of the arc plasma with the space-charge sheaths adjacent to the electrodes are applied to analyze in detail the plasma parameters and the phenomena occurring in the plasma column and the near-electrode regions of a DC arc generated in atmospheric pressure argon for current levels from 20 A up to 200 A. Results comprising electric field and potential, current density, heating of the electrodes, and effects of thermal and chemical non-equilibrium are presented and discussed. The current-voltage characteristic obtained is in fair agreement with known experimental data. It indicates a minimum for arc current of about 80 A. For all current levels, a field reversal in front of the anode accompanied by a voltage drop of (0.7-2.6) V is observed. Another field reversal is observed near the cathode for arc currents below 80 A.

Technetium-99m generator system

DOEpatents

Mirzadeh, Saed; Knapp, Jr., Furn F.; Collins, Emory D.

1998-01-01

A .sup.99 Mo/.sup.99m Tc generator system includes a sorbent column loaded with a composition containing .sup.99 Mo. The sorbent column has an effluent end in fluid communication with an anion-exchange column for concentrating .sup.99m Tc eluted from the sorbent column. A method of preparing a concentrated solution of .sup.99m Tc includes the general steps of: a. providing a sorbent column loaded with a composition containing .sup.99 Mo, the sorbent column having an effluent end in fluid communication with an anion-exchange column; b. eluting the sorbent column with a salt solution to elute .sup.99m Tc from the sorbent and to trap and concentrate the eluted .sup.99m Tc on the ion-exchange column; and c. eluting the concentrated .sup.99m Tc from the ion-exchange column with a solution comprising a reductive complexing agent.

Technetium-99m generator system

DOEpatents

Mirzadeh, S.; Knapp, F.F. Jr.; Collins, E.D.

1998-06-30

A {sup 99}Mo/{sup 99m}Tc generator system includes a sorbent column loaded with a composition containing {sup 99}Mo. The sorbent column has an effluent end in fluid communication with an anion-exchange column for concentrating {sup 99m}Tc eluted from the sorbent column. A method of preparing a concentrated solution of {sup 99m}Tc includes the general steps of: (a) providing a sorbent column loaded with a composition containing {sup 99}Mo, the sorbent column having an effluent end in fluid communication with an anion-exchange column; (b) eluting the sorbent column with a salt solution to elute {sup 99m}Tc from the sorbent and to trap and concentrate the eluted {sup 99m}Tc on the ion-exchange column; and (c) eluting the concentrated {sup 99m}Tc from the ion-exchange column with a solution comprising a reductive complexing agent. 1 fig.

Gyrokinetic-water-bag modeling of low-frequency instabilities in a laboratory magnetized plasma column

NASA Astrophysics Data System (ADS)

Gravier, E.; Klein, R.; Morel, P.; Besse, N.; Bertrand, P.

2008-12-01

A new model is presented, named collisional-gyro-water-bag (CGWB), which describes the collisional drift waves and ion-temperature-gradient (ITG) instabilities in a plasma column. This model is based on the kinetic gyro-water-bag approach recently developed [P. Morel et al., Phys. Plasmas 14, 112109 (2007)] to investigate ion-temperature-gradient modes. In CGWB electron-neutral collisions have been introduced and are now taken into account. The model has been validated by comparing CGWB linear analysis with other models previously proposed and experimental results as well. Kinetic effects on collisional drift waves are investigated, resulting in a less effective growth rate, and the transition from collisional drift waves to ITG instability depending on the ion temperature gradient is studied.

Micro liquid chromatography-mass spectrometry with direct liquid introduction used for separation and quantitation of all-trans- and 13-cis-retinoic acids and their 4-oxo metabolites in human plasma.

PubMed

Ranalder, U B; Lausecker, B B; Huselton, C

1993-07-23

The separation and quantitation of the pentafluorobenzyl derivatives of all-trans- and 13-cis-retinoic acids and their 4-oxo metabolites in human plasma on micro high-performance liquid chromatographic columns (0.32 mm I.D.) is described. The column outlet was directly coupled to the source of a quadrupole mass spectrometer via a simple SFC-frit interface. Negative ion chemical ionization conditions were obtained by coaxial introduction of ammonia as a reagent gas. A signal-to-noise ratio well above 3 was obtained for 1 pg of each analyte injected. The limit of quantitation calculated from spiked biological plasma extracts was 0.3 ng/ml.

Production of plasmas by long-wavelength lasers

DOEpatents

Dawson, J.M.

1973-10-01

A long-wavelength laser system for heating low-density plasma to high temperatures is described. In one embodiment, means are provided for repeatedly receiving and transmitting long-wavelength laser light in successive stages to form a laser-light beam path that repeatedly intersects with the equilibrium axis of a magnetically confined toroidal plasma column for interacting the laser light with the plasma for providing controlled thermonuclear fusion. Embodiments for heating specific linear plasmas are also provided. (Official Gazette)

Mode suppression of a two-dimensional potential relaxation instability in a weakly magnetized discharge plasma

NASA Astrophysics Data System (ADS)

Gyergyek, T.; Čerček, M.; Jelić, N.; Stanojević, M.

1993-05-01

A potential relaxation instability (PRI) is modulated by an external signal using an additional grid to modulate the radial plasma potential profile in a magnetized plasma column in a linear magnetized discharge plasma device. It is observed that the electrode current oscillations follow the van der Pol equation with an external forcing term, and the linear growth rate of the instability is measured.

Degenerate mixing of plasma waves on cold, magnetized single-species plasmas

NASA Astrophysics Data System (ADS)

Anderson, M. W.; O'Neil, T. M.; Dubin, D. H. E.; Gould, R. W.

2011-10-01

In the cold-fluid dispersion relation ω =ωp/[1+(k⊥/kz)2]1/2 for Trivelpiece-Gould waves on an infinitely long magnetized plasma cylinder, the transverse and axial wavenumbers appear only in the combination k⊥/kz. As a result, for any frequency ω <ωp, there are infinitely many degenerate waves, all having the same value of k⊥/kz. On a cold finite-length plasma column, these degenerate waves reflect into one another at the ends; thus, each standing-wave normal mode of the bounded plasma is a mixture of many degenerate waves, not a single standing wave as is often assumed. A striking feature of the many-wave modes is that the short-wavelength waves often add constructively along resonance cones given by dz /dr=±(ωp2/ω2-1)1/2. Also, the presence of short wavelengths in the admixture for a predominantly long-wavelength mode enhances the viscous damping beyond what the single-wave approximation would predict. Here, numerical solutions are obtained for modes of a cylindrical plasma column with rounded ends. Exploiting the fact that the modes of a spheroidal plasma are known analytically (the Dubin modes), a perturbation analysis is used to investigate the mixing of low-order, nearly degenerate Dubin modes caused by small deformations of a plasma spheroid.

Plasma Modes

NASA Astrophysics Data System (ADS)

Dubin, D. H. E.

This chapter explores several aspects of the linear electrostatic normal modes of oscillation for a single-species non-neutral plasma in a Penning trap. Linearized fluid equations of motion are developed, assuming the plasma is cold but collisionless, which allow derivation of the cold plasma dielectric tensor and the electrostatic wave equation. Upper hybrid and magnetized plasma waves in an infinite uniform plasma are described. The effect of the plasma surface in a bounded plasma system is considered, and the properties of surface plasma waves are characterized. The normal modes of a cylindrical plasma column are discussed, and finally, modes of spheroidal plasmas, and finite temperature effects on the modes, are briefly described.

Assessment of quasi-linear effect of RF power spectrum for enabling lower hybrid current drive in reactor plasmas

NASA Astrophysics Data System (ADS)

Cesario, Roberto; Cardinali, Alessandro; Castaldo, Carmine; Amicucci, Luca; Ceccuzzi, Silvio; Galli, Alessandro; Napoli, Francesco; Panaccione, Luigi; Santini, Franco; Schettini, Giuseppe; Tuccillo, Angelo Antonio

2017-10-01

The main research on the energy from thermonuclear fusion uses deuterium plasmas magnetically trapped in toroidal devices. To suppress the turbulent eddies that impair thermal insulation and pressure tight of the plasma, current drive (CD) is necessary, but tools envisaged so far are unable accomplishing this task while efficiently and flexibly matching the natural current profiles self-generated at large radii of the plasma column [1-5]. The lower hybrid current drive (LHCD) [6] can satisfy this important need of a reactor [1], but the LHCD system has been unexpectedly mothballed on JET. The problematic extrapolation of the LHCD tool at reactor graded high values of, respectively, density and temperatures of plasma has been now solved. The high density problem is solved by the FTU (Frascati Tokamak Upgrade) method [7], and solution of the high temperature one is presented here. Model results based on quasi-linear (QL) theory evidence the capability, w.r.t linear theory, of suitable operating parameters of reducing the wave damping in hot reactor plasmas. Namely, using higher RF power densities [8], or a narrower antenna power spectrum in refractive index [9,10], the obstacle for LHCD represented by too high temperature of reactor plasmas should be overcome. The former method cannot be used for routinely, safe antenna operations, Thus, only the latter key is really exploitable in a reactor. The proposed solutions are ultimately necessary for viability of an economic reactor.

Toward validation of a 3-D plasma turbulence model using LAPD data

NASA Astrophysics Data System (ADS)

Umansky, M. V.

2010-11-01

Detailed results from a 3-D fluid simulation of plasma turbulence are compared with experimental data from the Large Plasma Device (LAPD) at UCLA. LAPD is a magnetized plasma column experiment with a high repetition rate, allowing detailed time-and-space resolved probe data on plasma turbulence and transport. The large amount of data allows a thorough comparison with the simulation results. For the observed drift-type modes, LAPD plasmas are strongly collisional (φ*/νei1 and λei/L1), providing justification for a fluid treatment. Accordingly, the model is based on reduced Braginskii equations and is implemented in the framework of the BOUT code, originally developed at LLNL for tokamak edge plasmas. Analysis of linear plasma instabilities shows that resistive drift modes, rotation-driven interchange modes, and Kelvin-Helmholtz modes can all be important in LAPD and have comparable frequencies and growth rates. In nonlinear simulations using measured LAPD density profiles, evolution of instabilities and self-generated zonal flows results in a saturated turbulent state. Comparisons of these simulations with measurements in LAPD plasmas reveal good agreement, in particular in the frequency spectrum, spatial correlation, and amplitude probability distribution function of density fluctuations. Also, consistent with the experiment, the simulations indicate a great deal of similarity between plasma turbulence in LAPD and some features of tokamak edge turbulence. Similar to tokamak edge plasmas, density transport appears to be predominantly carried by large particle-flux events. Despite the intermittent character of the calculated turbulence, as indicated by fluctuation statistics, the turbulent particle flux is consistent with a diffusive model with diffusion coefficient close to the Bohm value.

Split Flow Online Solid-Phase Extraction Coupled with Inductively Coupled Plasma Mass Spectrometry System for One-Shot Data Acquisition of Quantification and Recovery Efficiency.

PubMed

Furukawa, Makoto; Takagai, Yoshitaka

2016-10-04

Online solid-phase extraction (SPE) coupled with inductively coupled plasma mass spectrometry (ICPMS) is a useful tool in automatic sequential analysis. However, it cannot simultaneously quantify the analytical targets and their recovery percentages (R%) in one-shot samples. We propose a system that simultaneously acquires both data in a single sample injection. The main flowline of the online solid-phase extraction is divided into main and split flows. The split flow line (i.e., bypass line), which circumvents the SPE column, was placed on the main flow line. Under program-controlled switching of the automatic valve, the ICPMS sequentially measures the targets in a sample before and after column preconcentration and determines the target concentrations and the R% on the SPE column. This paper describes the system development and two demonstrations to exhibit the analytical significance, i.e., the ultratrace amounts of radioactive strontium ( 90 Sr) using commercial Sr-trap resin and multielement adsorbability on the SPE column. This system is applicable to other flow analyses and detectors in online solid phase extraction.

External control of ion waves in a plasma by high frequency fields

DOEpatents

Kaw, P.K.; Dawson, J.M.

1973-12-18

An apparatus and method are described for stabilizing plasma instabilities, in a magnetically confined plasma column by transmitting into the plasma high frequency electromagnetic waves at a frequency close to the electron plasma frequency. The said frequencies, e.g., are between the plasma frequency and 1.5 times the plasma frequency at a power level below the level for producing parametric instabilities in a plasma having temperatures from below 10 eV to about 10 keV or more, at densities from below 10/sup 13/ to above 10/sup 18/ particles/cm/sup 3/. (Official Gazette)

Simplified and Efficient Quantification of Low-abundance Proteins at Very High Multiplex via Targeted Mass Spectrometry*

PubMed Central

Burgess, Michael W.; Keshishian, Hasmik; Mani, D. R.; Gillette, Michael A.; Carr, Steven A.

2014-01-01

Liquid chromatography–multiple reaction monitoring mass spectrometry (LC-MRM-MS) of plasma that has been depleted of abundant proteins and fractionated at the peptide level into six to eight fractions is a proven method for quantifying proteins present at low nanogram-per-milliliter levels. A drawback of fraction-MRM is the increased analysis time due to the generation of multiple fractions per biological sample. We now report that the use of heated, long, fused silica columns (>30 cm) packed with 1.9 μm of packing material can reduce or eliminate the need for fractionation prior to LC-MRM-MS without a significant loss of sensitivity or precision relative to fraction-MRM. We empirically determined the optimal column length, temperature, gradient duration, and sample load for such assays and used these conditions to study detection sensitivity and assay precision. In addition to increased peak capacity, longer columns packed with smaller beads tolerated a 4- to 6-fold increase in analyte load without a loss of robustness or reproducibility. The longer columns also provided a 4-fold improvement in median limit-of-quantitation values with increased assay precision relative to the standard 12 cm columns packed with 3 μm material. Overall, the optimized chromatography provided an approximately 3-fold increase in analysis throughput with excellent robustness and less than a 2-fold reduction in quantitative sensitivity relative to fraction-MRM. The value of the system for increased multiplexing was demonstrated by the ability to configure an 800-plex MRM-MS assay, run in a single analysis, comprising 2400 transitions with retention time scheduling to monitor 400 unlabeled and heavy labeled peptide pairs. PMID:24522978

Evaluation and routine application of the novel restricted-access precolumn packing material Alkyl-Diol Silica: coupled-column high-performance liquid chromatographic analysis of the photoreactive drug 8-methoxypsoralen in plasma.

PubMed

Vielhauer, S; Rudolphi, A; Boos, K S; Seidel, D

1995-04-21

A fully automated coupled-column HPLC method for on-line sample processing and determination of the photoreactive drug 8-methoxypsoralen (8-MOP) in plasma has been developed. The method is based on the novel internal-surface reversed-phase precolumn packing materials Alkyl-Diol Silica (ADS). This new family of restricted-access materials has a hydrophilic, electroneutral outer particle surface and a hydrophobic internal pore surface. The supports tolerate the direct and repetitive injection of proteinaceous fluids such as plasma and allow a classical C18-, C8- or C4-reversed-phase partitioning at the internal (pore) surface. The total protein load, i.e. the lifetime of the precolumn used in this study (C8-Alkyl-Diol Silica, 25 microns, 25 x 4 mm I.D.), exceeds more than 100 ml of plasma. 8-MOP was detected by its native fluorescence (excitation 312 nm, emission 540 nm). Validation of the method revealed a quantitative and matrix-independent recovery (99.5-101.3% measured at five concentrations between 21.3 and 625.2 ng of 8-MOP per milliliter of plasma), linearity over a wide range of 8-MOP concentrations (1.2-3070 ng of 8-MOP/ml, r = 0.999), low limits of detection (0.39 ng of 8-MOP/ml) and quantitation (0.79 ng of 8-MOP/ml) and a high between-run (C.V. 1.47%, n = 10) and within-run (C.V. 1.33%, n = 10) reproducibility. This paper introduces coupled-column HPLC as a suitable method for on-site analysis of drug plasma profiles (bedside-monitoring).

Identification and control of plasma vertical position using neural network in Damavand tokamak.

PubMed

Rasouli, H; Rasouli, C; Koohi, A

2013-02-01

In this work, a nonlinear model is introduced to determine the vertical position of the plasma column in Damavand tokamak. Using this model as a simulator, a nonlinear neural network controller has been designed. In the first stage, the electronic drive and sensory circuits of Damavand tokamak are modified. These circuits can control the vertical position of the plasma column inside the vacuum vessel. Since the vertical position of plasma is an unstable parameter, a direct closed loop system identification algorithm is performed. In the second stage, a nonlinear model is identified for plasma vertical position, based on the multilayer perceptron (MLP) neural network (NN) structure. Estimation of simulator parameters has been performed by back-propagation error algorithm using Levenberg-Marquardt gradient descent optimization technique. The model is verified through simulation of the whole closed loop system using both simulator and actual plant in similar conditions. As the final stage, a MLP neural network controller is designed for simulator model. In the last step, online training is performed to tune the controller parameters. Simulation results justify using of the NN controller for the actual plant.

[Determination of acyclovir in mouse plasma and tissues by reversed-phase high performance liquid chromatography].

PubMed

Xu, Y; Zhou, S W; Tang, J L; Huang, L Q

2001-11-01

The aim of this study was to establish an high performance liquid chromatographic method for determining acyclovir (ACV) concentration in mouse plasma and tissues. A solution of 0.25 mL 60 g/L perchloric acid and 0.25 mL acetonitrile was added into 0.2 mL plasma or 0.2 g tissues to precipitate proteins. Following centrifugation, the supernatant obtained was injected into a reversed-phase column. Operating conditions were Hypersil ODS column(250 mm x 4.6 mm i.d., 5 microns), methanol-water-acetic acid(1:99:0.5, volume ratio) solution as mobile phase at a flow rate of 1.5 mL/min, UV detection at 252 nm. The detection limit of ACV concentration in plasma was 20 micrograms/L and that in tissues was 50 ng/g. The standard curves for ACV were linear in plasma and homogenate of tissues (r > 0.99). The precision of the method was good and the recoveries of ACV were higher than 97.5%. So this method is rapid, accurate and convenient for determination of ACV concentrations in plasma and tissues.

Beam deviation method as a diagnostic tool for the plasma focus.

PubMed

Schmidt, H; Rückle, B

1978-04-15

The application of an optical method for density measurements in cylindrical plasmas is described. The angular deviation of a probing light beam sent through a plasma is proportional to the maximum of the density in the plasma column. The deviation does not depend on the plasma dimensions; however, it is influenced to a certain degree by the density profile. The method is successfully applied to the investigation of a dense plasma focus with a time resolution of 2 nsec and a spatial resolution (in axial direction) of 2 mm.

Interplay between intrinsic plasma rotation and magnetic island evolution in disruptive discharges

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ronchi, G.; Severo, J. H. F.; Salzedas, F.

The behavior of the intrinsic toroidal rotation of the plasma column during the growth and eventual saturation of m/n = 2/1 magnetic islands, triggered by programmed density rise, has been carefully investigated in disruptive discharges in TCABR. The results show that, as the island starts to grow and rotate at a speed larger than that of the plasma column, the angular frequency of the intrinsic toroidal rotation increases and that of the island decreases, following the expectation of synchronization. As the island saturates at a large size, just before a major disruption, the angular speed of the intrinsic rotation decreasesmore » quite rapidly, even though the island keeps still rotating at a reduced speed. This decrease of the toroidal rotation is quite reproducible and can be considered as an indicative of disruption.« less

Evolution of space open electric arc burning in the external axial magnetic field

NASA Astrophysics Data System (ADS)

Urusova, I. R.; Urusova, T. E.

2018-06-01

The calculation was made for open DC electric arc burning in an external uniform axial magnetic field. It was performed within the framework of a nonstationary three-dimensional mathematical model in approximation of partial local thermodynamic equilibrium of plasma. A "schematic" analog of electron temperature fluctuations was proposed for numerical realization of the open electric arc column of a helical shape. According to calculations, it was established that the column of the open electric arc takes a helical space shape. Plasma rotates around a longitudinal axis of the arc, at that the directions of plasma rotation near the cathode and the anode are opposite. In the arc cross-sections, the velocity of plasma rotation is unequal and the deviation value of the same part of the arc from the central axis varies in time. A helical shape of the open arc is not stable and varies in time. Apparently, the open arc cannot remain stable and invariable in the time helical shape in the external axial magnetic field.

Studies of a driven Alfvénic cavity and cylindrical Alfven eigenmodes in LAPD

NASA Astrophysics Data System (ADS)

Lybarger, Warren; Carter, Troy; Brugman, Brian; Pribyl, Pat

2004-11-01

An Alfven wave MASER has been observed in the Large Plasma Device (LAPD), where an instability drives a resonant Alfven wave in the cavity defined by the cathode and anode of the discharge source(J.E. Maggs and G.J. Morales, PRL, 91, 035004-1 (2003)). We will present a study of external driving of this cavity, motivated by a desire to find a source of large amplitude Alfvén waves for studies of nonlinear interactions. The cavity is driven by modulating the discharge current using a broadband, high power push-pull amplifier. The Alfvén waves launched by exciting the cavity are large amplitude (δ B/B ˜ 1%) and are eigenmodes of the cylindrical column. Experimental results will be presented on the structure of the eigenmodes in the plasma column, the Q-value of the cavity and its dependence on plasma parameters, and deviations in the structure of the eigenmodes from ideal MHD due to kinetic effects. Experimental results will be compared to theories of Alfvén eigenmodes in a cylindrical column. * Work supported by DOE grant # DE-FG03-02ER54688

Increase in the neutron yield from a dense plasma-focus experiment performed with a conical tip placed in the centre of the anode end

NASA Astrophysics Data System (ADS)

Kubes, P.; Paduch, M.; Cikhardt, J.; Cikhardtova, B.; Klir, D.; Kravarik, J.; Rezac, K.; Zielinska, E.; Sadowski, M. J.; Szymaszek, A.; Tomaszewski, K.; Zaloga, D.

2017-09-01

The paper describes the evolution of self-organized structures inside a pinched plasma column during the phase of the effective production of fusion neutrons, as observed in the mega-ampere plasma focus experiment performed with a conical tip placed in the centre of the anode face. In a comparison with the plane anode face configuration, the described anode shape facilitated transformations in the pinch column during the neutron production and increased the neutron yield several times. Simultaneously, it decreased the minimal diameter and the length of the pinched column, and it depressed the first neutron pulse. It also induced shorter pulses of X-rays and neutrons, which enabled the determination of a temporal difference between the emission of electron and deuteron beams. The fast electrons were produced mainly during a disruption of the pinch constriction, while the fast deuterons - during the formation and explosion of plasmoids. The paper also presents the temporal evolution of a current distribution in the plasmoid during the neutron production, as well as the appearance and stable positions of current filaments traces upon the surface of the conical anode tip.

Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC-Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma.

PubMed

El-Zaher, Asmaa A; Mahrouse, Marianne A

2013-01-01

A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescamine reagent (0.01%, 1.5 mL) and borate buffer (pH 8.5, 2 mL). Experimental design was applied for optimization of the derivatization step. The resulting highly fluorescent stable derivative was chromatographed on C18 column and eluted using methanol:water (60:40, v/v) at a flow rate of 1 mL min(-1). A fluorescence detector (λex 390 and λem 480 nm) was used. The method was linear over the concentration range 20-200 ng mL(-1). The structure of the fluorescent product was proposed, the method was then validated and applied for the determination of TOB in human plasma. The results were statistically compared with the reference method, revealing no significant difference.

Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma

PubMed Central

El-Zaher, Asmaa A.; Mahrouse, Marianne A.

2013-01-01

A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescamine reagent (0.01%, 1.5 mL) and borate buffer (pH 8.5, 2 mL). Experimental design was applied for optimization of the derivatization step. The resulting highly fluorescent stable derivative was chromatographed on C18 column and eluted using methanol:water (60:40, v/v) at a flow rate of 1 mL min−1. A fluorescence detector (λex 390 and λem 480 nm) was used. The method was linear over the concentration range 20–200 ng mL−1. The structure of the fluorescent product was proposed, the method was then validated and applied for the determination of TOB in human plasma. The results were statistically compared with the reference method, revealing no significant difference. PMID:23700362

Isotopic generator for bismuth-212 and lead-212 based on radium

DOEpatents

Hines, J.J.; Atcher, R.W.; Friedman, A.M.

1985-01-30

Disclosed are method and apparatus for providing radionuclides of bismuth-212 and lead-212. Thorium-228 and carrier solution starting material is input to a radiologically contained portion of an isotopic generator system, and radium-224 is separated from thorium-228 which is retained by a strongly basic anion exchange column. The separated radium-224 is transferred to an accessible, strongly acidic cationic exchange column. The cationic column retains the radium-224, and natural radioactive decay generates bismuth-212 and lead-212. The cationic exchange column can also be separated from the contained portion of the system and utilized without the extraordinary safety measures necessary in the contained portion. Furthermore, the cationic exchange column provides over a relatively long time period the short lived lead-212 and bismuth-212 radionuclides which are useful for a variety of medical therapies.

Isotopic generator for bismuth-212 and lead-212 from radium

DOEpatents

Atcher, Robert W.; Friedman, Arnold M.; Hines, John

1987-01-01

A method and apparatus for providing radionuclides of bismuth-212 and lead-212. Thorium-228 and carrier solution starting material is input to a radiologically contained portion of an isotopic generator system, and radium-224 is separated from thorium-228 which is retained by a strongly basic anion exchange column. The separated radium-224 is transferred to an accessible, strongly acidic cationic exchange column. The cationic column retains the radium-224, and natural radioactive decay generates bismuth-212 and lead-212. The cationic exchange column can also be separated from the contained portion of the system and utilized without the extraordinary safety measures necessary in the contained portion. Furthermore, the cationic exchange column provides over a relatively long time period the short lived lead-212 and bismuth-212 radionuclides which are useful for a variety of medical therapies.

Experiments on Plasma Turbulence Created by Supersonic Plasma Flows with Shear

DTIC Science & Technology

2014-04-01

for producing a plasma column (in black). An insulated wire traverses the plasma and car - ries a pulsed current in x-direction. The unmagnetized ions... electric field which together with the B field around the wire causes an electron ExB drift. The ions are unmagnetized. A radial space charge electric field...by the self-consistent currents passing through the grid. These currents, consisting of electron and ion flows, are controlled by the electrical

Fully automated methods for the determination of hydrochlorothiazide in human plasma and urine.

PubMed

Hsieh, J Y; Lin, C; Matuszewski, B K; Dobrinska, M R

1994-12-01

LC assays utilizing fully automated sample preparation procedures on Zymark PyTechnology Robot and BenchMate Workstation for the quantification of hydrochlorothiazide (HCTZ) in human plasma and urine have been developed. After aliquoting plasma and urine samples, and adding internal standard (IS) manually, the robot executed buffer and organic solvent addition, liquid-liquid extraction, solvent evaporation and on-line LC injection steps for plasma samples, whereas, BenchMate performed buffer and organic solvent addition, liquid-liquid and solid-phase extractions, and on-line LC injection steps for urine samples. Chromatographic separations were carried out on Beckman Octyl Ultrasphere column using the mobile phase composed of 12% (v/v) acetonitrile and 88% of either an ion-pairing reagent (plasma) or 0.1% trifluoroacetic acid (urine). The eluent from the column was monitored with UV detector (271 nm). Peak heights for HCTZ and IS were automatically processed using a PE-Nelson ACCESS*CHROM laboratory automation system. The assays have been validated in the concentration range of 2-100 ng ml-1 in plasma and 0.1-20 micrograms ml-1 in urine. Both plasma and urine assays have the sensitivity and specificity necessary to determine plasma and urine concentrations of HCTZ from low dose (6.25/12.5 mg) administration of HCTZ to human subjects in the presence or absence of losartan.

Dynamics of conical wire array Z-pinch implosions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ampleford, D. J.; Lebedev, S. V.; Bland, S. N.

2007-10-15

A modification of the wire array Z pinch, the conical wire array, has applications to the understanding of wire array implosions and potentially to pulse shaping relevant to inertial confinement fusion. Results are presented from imploding conical wire array experiments performed on university scale 1 MA generators--the MAGPIE generator (1 MA, 240 ns) at Imperial College London [I. H. Mitchell et al., Rev. Sci Instrum. 67, 1533 (1996)] and the Nevada Terawatt Facility's Zebra generator (1 MA, 100 ns) at the University of Nevada, Reno [B. Bauer et al., in Dense Z-Pinches, edited by N. Pereira, J. Davis, and P.more » Pulsifer (AIP, New York, 1997), Vol. 409, p. 153]. This paper will discuss the implosion dynamics of conical wire arrays. Data indicate that mass ablation from the wires in this complex system can be reproduced with a rocket model with fixed ablation velocity. Modulations in the ablated plasma are present, the wavelength of which is invariant to a threefold variation in magnetic field strength. The axial variation in the array leads to a zippered precursor column formation. An initial implosion of a magnetic bubble near the cathode is followed by the implosion zippering upwards. Spectroscopic data demonstrating a variation of plasma parameters (e.g., electron temperature) along the Z-pinch axis is discussed, and experimental data are compared to magnetohydrodynamic simulations.« less

Column Selection for Biomedical Analysis Supported by Column Classification Based on Four Test Parameters

PubMed Central

Plenis, Alina; Rekowska, Natalia; Bączek, Tomasz

2016-01-01

This article focuses on correlating the column classification obtained from the method created at the Katholieke Universiteit Leuven (KUL), with the chromatographic resolution attained in biomedical separation. In the KUL system, each column is described with four parameters, which enables estimation of the FKUL value characterising similarity of those parameters to the selected reference stationary phase. Thus, a ranking list based on the FKUL value can be calculated for the chosen reference column, then correlated with the results of the column performance test. In this study, the column performance test was based on analysis of moclobemide and its two metabolites in human plasma by liquid chromatography (LC), using 18 columns. The comparative study was performed using traditional correlation of the FKUL values with the retention parameters of the analytes describing the column performance test. In order to deepen the comparative assessment of both data sets, factor analysis (FA) was also used. The obtained results indicated that the stationary phase classes, closely related according to the KUL method, yielded comparable separation for the target substances. Therefore, the column ranking system based on the FKUL-values could be considered supportive in the choice of the appropriate column for biomedical analysis. PMID:26805819

Column Selection for Biomedical Analysis Supported by Column Classification Based on Four Test Parameters.

PubMed

Plenis, Alina; Rekowska, Natalia; Bączek, Tomasz

2016-01-21

This article focuses on correlating the column classification obtained from the method created at the Katholieke Universiteit Leuven (KUL), with the chromatographic resolution attained in biomedical separation. In the KUL system, each column is described with four parameters, which enables estimation of the FKUL value characterising similarity of those parameters to the selected reference stationary phase. Thus, a ranking list based on the FKUL value can be calculated for the chosen reference column, then correlated with the results of the column performance test. In this study, the column performance test was based on analysis of moclobemide and its two metabolites in human plasma by liquid chromatography (LC), using 18 columns. The comparative study was performed using traditional correlation of the FKUL values with the retention parameters of the analytes describing the column performance test. In order to deepen the comparative assessment of both data sets, factor analysis (FA) was also used. The obtained results indicated that the stationary phase classes, closely related according to the KUL method, yielded comparable separation for the target substances. Therefore, the column ranking system based on the FKUL-values could be considered supportive in the choice of the appropriate column for biomedical analysis.

Review of controlled fusion research using laser heating.

NASA Technical Reports Server (NTRS)

Hertzberg, A.

1973-01-01

Development of methods for generating high laser pulse energy has stimulated research leading to new ideas for practical controlled thermonuclear fusion machines. A review is presented of some important efforts in progress, and two different approaches have been selected as examples for discussion. One involves the concept of very short pulse lasers with power output tailored, in time, to obtain a nearly isentropic compression of a deuterium-tritium pellet to very high densities and temperatures. A second approach utilizing long wavelength, long pulse, efficient gas lasers to heat a column of plasma contained in a solenoidal field is also discussed. The working requirements of the laser and various magnetic field geometries of this approach are described.

PLASMA PHYSICS AND STATISTICAL MECHANICS IN BRUSSELS, BELGIUM,

DTIC Science & Technology

significant research in the theory and experiment of the Tonks-Dattner resonances in a cylindrical plasma column. The second visit was to Professors I ...Prigogine and R. Balescu , of the Faculte des Sciences, Universite Libre de Bruxelles, who together direct a large group of scientists working on all

A theoretical study of hot plasma spheroids in the presence of low-frequency electromagnetic waves

NASA Astrophysics Data System (ADS)

Ahmadizadeh, Y.; Jazi, B.; Barjesteh, S.

2016-07-01

While taking into account thermal motion of electrons, scattering of electromagnetic waves with low frequency from hot plasma spheroids is investigated. In this theoretical research, ions are heavy to respond to electromagnetic fluctuations. The solution of scalar wave equation in spheroidal coordinates for electric potential inside the plasma spheroids are obtained. The variations of resonance frequencies vs. Debye length are studied and consistency between the obtained results in this paper and the results for the well-known plasma objects such as plasma column and spherical plasma have been proved.

Time-resolved characteristics of deuteron-beam generated by plasma focus discharge.

PubMed

Lim, Lian-Kuang; Yap, Seong-Ling; Bradley, D A

2018-01-01

The plasma focus device discussed herein is a Z-pinch pulsed-plasma arrangement. In this, the plasma is heated and compressed into a cylindrical column, producing a typical density of > 1025 particles/m3 and a temperature of (1-3) × 107 oC. The plasma focus has been widely investigated as a radiation source, including as ion-beams, electron-beams and as a source of x-ray and neutron production, providing considerable scope for use in a variety of technological situations. Thus said, the nature of the radiation emission depends on the dynamics of the plasma pinch. In this study of the characteristics of deuteron-beam emission, in terms of energy, fluence and angular distribution were analyzed. The 2.7 kJ plasma focus discharge has been made to operate at a pressure of less than 1 mbar rather than at its more conventional operating pressure of a few mbar. Faraday cup were used to determine deuteron-beam energy and deuteron-beam fluence per shot while CR-39 solid-state nuclear track detectors were employed in studying the angular distribution of deuteron emission. Beam energy and deuteron-beam fluence per shot have been found to be pressure dependent. The largest value of average deuteron energy measured for present conditions was found to be (52 ± 7) keV, while the deuteron-beam fluence per shot was of the order of 1015 ions/m2 when operated at a pressure of 0.2 mbar. The deuteron-beam emission is in the forward direction and is observed to be highly anisotropic.

Time-resolved characteristics of deuteron-beam generated by plasma focus discharge

PubMed Central

Bradley, D. A.

2018-01-01

The plasma focus device discussed herein is a Z-pinch pulsed-plasma arrangement. In this, the plasma is heated and compressed into a cylindrical column, producing a typical density of > 1025 particles/m3 and a temperature of (1–3) × 107 oC. The plasma focus has been widely investigated as a radiation source, including as ion-beams, electron-beams and as a source of x-ray and neutron production, providing considerable scope for use in a variety of technological situations. Thus said, the nature of the radiation emission depends on the dynamics of the plasma pinch. In this study of the characteristics of deuteron-beam emission, in terms of energy, fluence and angular distribution were analyzed. The 2.7 kJ plasma focus discharge has been made to operate at a pressure of less than 1 mbar rather than at its more conventional operating pressure of a few mbar. Faraday cup were used to determine deuteron-beam energy and deuteron-beam fluence per shot while CR-39 solid-state nuclear track detectors were employed in studying the angular distribution of deuteron emission. Beam energy and deuteron-beam fluence per shot have been found to be pressure dependent. The largest value of average deuteron energy measured for present conditions was found to be (52 ± 7) keV, while the deuteron-beam fluence per shot was of the order of 1015 ions/m2 when operated at a pressure of 0.2 mbar. The deuteron-beam emission is in the forward direction and is observed to be highly anisotropic. PMID:29309425

Experimental results from plasma transport on Prototype-Material Plasma Exposure eXperiment and comparison with B2-Eirene modeling

NASA Astrophysics Data System (ADS)

Kafle, N.; Caneses, J. F.; Biewer, T. M.; Owen, L.; Showers, M.; Donovan, D.; Caughman, J. B.; Goulding, R. H.; Rapp, Juergen

2017-10-01

Proto-MPEX at ORNL is a linear plasma device that combines a helicon plasma source with additional microwave and RF heating to deliver high plasma heat and particle fluxes to a target. Double Langmuir probes and Thomson scattering are being used to measure local Te and ne at various radial and axial locations. A recently constructed Mach- double probe provides the added capability of simultaneously measuring Te, ne, and Mach number. With this diagnostic, it is possible to infer the plasma flow, particle flux, and convective heat flux at different locations along the plasma column in Proto-MPEX. Preliminary results show Mach numbers of 0.6 and 0.8 in either direction away from the helicon source, and no flow near the source for the case where the peak magnetic field was 1.0 T. In addition, the Thomson Scattering system has been upgraded to measure ne and Te profiles at two axial locations, upstream at the electron heating location and downstream close to the target. Measurements of particle flow and flux profiles, heat flux, and profiles of ne and Te will be discussed. The extensive coverage provided by these diagnostics permits data-constrained B2-Eirene modeling of the entire plasma column, and comparison with results of modeling of high density mode plasmas will be presented. Supported by the US. D.O.E. contract DE-AC05-00OR22725.

Development and validation of a reversed-phase fluorescence HPLC method for determination of bucillamine in human plasma using pre-column derivatization with monobromobimane.

PubMed

Lee, Kang Choon; Chun, Young Goo; Kim, Insoo; Shin, Beom Soo; Park, Eun-Seok; Yoo, Sun Dong; Youn, Yu Seok

2009-07-15

A simple, specific and sensitive derivatization with monobromobimane (mBrB) and the corresponding HPLC-fluorescence quantitation method for the analysis of bucillamine in human plasma was developed and validated. The analytical procedure involves a simple protein precipitation, pre-column fluorescence derivatization, and separation by reversed-phase high performance liquid chromatography (RP-HPLC). The calibration curve showed good linearity over a wide concentration range (50 ng/mL to 10 microg/mL) in human plasma (r(2)=0.9998). The lower limit of quantitation (LLOQ) was 50 ng/mL. The average precision and accuracy at LLOQ were within 6.3% and 107.6%, respectively. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (300 mg) of bucillamine to 20 healthy Korean volunteers.

A rapid and sensitive method for the quantitation of montelukast in sheep plasma using liquid chromatography/tandem mass spectrometry.

PubMed

Papp, Robert; Luk, Pauline; Mullett, Wayne M; Kwong, Elizabeth

2007-10-15

A rapid LC-MS/MS method was developed and partially validated for the quantitation of montelukast in spiked sheep plasma. A total run time of 1.5 min was achieved using a short monolithic column and employing a rapid gradient. Sample preparation involved protein precipitation with twofold acetonitrile by volume during which a deuterated internal standard (montelukast D-6) was incorporated. The MRM transitions for montelukast and the deuterated internal standard were 586/422 and 592/427, respectively. A linear dynamic range of 0.25-500 ng/mL with a correlation coefficient of 0.9999 was achieved. Precision was below 5% at all levels except at the LOQ (0.36 ng/mL) which demonstrated an overall of R.S.D. of 8%. Post-column infusion experiments were performed with precipitated plasma matrix and showed minimal interference with the peaks of interest.

Enantiospecific determination of PNU-83894 and its major metabolite, PNU-83892, in plasma, and its application to the characterization of the enantioselective pharmacokinetics of PNU-83894 in the dog.

PubMed

Zhong, W Z; Williams, M G

2000-02-25

A chiral method for the simultaneous analysis of the (+)- and (-)-enantiomers of PNU-83894 and its metabolite, PNU-83892, in plasma was developed to characterize the enantioselective pharmacokinetics of PNU-83894, a potential anticonvulsant candidate. The method involves solid-phase extraction (phenyl column) of the enantiomers from plasma followed by direct enantioselective separation on a beta-cyclodextrin HPLC chiral column and UV detection at 230 nm. The linear range for this method was found to be 12.5 ng/ml to 5.00 microg/ml and the intra- and inter-assay precision and accuracy for each enantiomer were <11% in all cases. The validity of this assay was also demonstrated by its application to the pharmacokinetic evaluation of PNU-83894 in the dog.

[Dyuamical studies on metabolic chemistry of lignans from seeds of Arctium lappa].

PubMed

Zheng, Yi-min; Cai, Shao-xi; Xu, Xiu-ying; Fu, Shan-quan

2005-08-01

To study the metabolic chemistry and pharmaco-dynamics characters of ligan from seeds of Arctium lappa. HPLC method was used in the study. The analysis was carried out on C18 column. The mobile phase was CH3CN-0.05% H3PO4 (36:64) with flow-rate at 0.6 mL x min(-1) and wave-length of 210 nm. The column temperature was kept at 25 degrees C. The results indicated that the ligan was detected in plasma and the main organs 5 min after po. The main metabolic production in plasma was arctigenin. In addition, arctigenin and an unknown product were found in metabolic production in the organs. The method was stable,simple and reproducible. It can be used to determine the metabolic product of the ligan. The metabolic chemistry of ligan in plasma was obviously different from that in the main organs.

Oriented immobilized anti-hIgG via F(c) fragment-imprinted PHEMA cryogel for IgG purification.

PubMed

Bereli, Nilay; Ertürk, Gizem; Tümer, M Aşkin; Say, Ridvan; Denizli, Adil

2013-05-01

Antibodies are used in many applications, especially as diagnostic and therapeutic agents. Among the various techniques used for the purification of antibodies, immunoaffinity chromatography is by far the most common. For this purpose, oriented immobilization of antibodies is an important step for the efficiency of purification step. In this study, F(c) fragment-imprinted poly(hydroxyethyl methacrylate) cryogel (MIP) was prepared for the oriented immobilization of anti-hIgG for IgG purification from human plasma. Non-imprinted poly(hydroxyethyl methacrylate) cryogel (NIP) was also prepared for random immobilization of anti-hIgG to compare the adsorption capacities of oriented (MIP/anti-hIgG) and random (NIP/anti-hIgG) cryogel columns. The amount of immobilized anti-hIgG was 19.8 mg/g for the NIP column and 23.7 mg/g for the MIP column. Although the amount of immobilized anti-hIgG was almost the same for the NIP and MIP columns, IgG adsorption capacity was found to be three times higher than the NIP/anti-hIgG column (29.7 mg/g) for the MIP/anti-hIgG column (86.9 mg/g). Higher IgG adsorption capacity was observed from human plasma (up to 106.4 mg/g) with the MIP/anti-hIgG cryogel column. Adsorbed IgG was eluted using 1.0 M NaCl with a purity of 96.7%. The results obtained here are very encouraging and showed the usability of MIP/anti-hIgG cryogel prepared via imprinting of Fc fragments as an alternative to conventional immunoaffinity techniques for IgG purification. Copyright © 2012 John Wiley & Sons, Ltd.

Simultaneous targeted analysis of trimethylamine-N-oxide, choline, betaine, and carnitine by high performance liquid chromatography tandem mass spectrometry.

PubMed

Liu, Jia; Zhao, Mingming; Zhou, Juntuo; Liu, Changjie; Zheng, Lemin; Yin, Yuxin

2016-11-01

Trimethylamine-N-oxide (TMAO) is a metabolite generated from choline, betaine and carnitine in a gut microbiota-dependent way. This molecule is associated with development of atherosclerosis and cardiovascular events. A sensitive liquid chromatographic electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) has been developed and validated for the simultaneous determination of TMAO related molecules including TMAO, betaine, choline, and carnitine in mouse plasma. Analytes are extracted after protein precipitation by methanol and subjected to LC-ESI-MS/MS without preliminary derivatization. Separation of analytes was achieved on an amide column with acetonitrile-water as the mobile phase. This method has been fully validated in this study in terms of selectivity, linearity, sensitivity, precision, accuracy, and carryover effect, and the stability of the analyte under various conditions has been confirmed. This developed method has successfully been applied to plasma samples of our mouse model. Copyright © 2016 Elsevier B.V. All rights reserved.

Electrostatic ion-cyclotron waves in a nonuniform magnetic field

NASA Technical Reports Server (NTRS)

Cartier, S. L.; Dangelo, N.; Merlino, R. L.

1985-01-01

The properties of electrostatic ion-cyclotron waves excited in a single-ended cesium Q machine with a nonuniform magnetic field are described. The electrostatic ion-cyclotron waves are generated in the usual manner by drawing an electron current to a small exciter disk immersed in the plasma column. The parallel and perpendicular (to B) wavelengths and phase velocities are determined by mapping out two-dimensional wave phase contours. The wave frequency f depends on the location of the exciter disk in the nonuniform magnetic field, and propagating waves are only observed in the region where f is approximately greater than fci, where fci is the local ion-cyclotron frequency. The parallel phase velocity is in the direction of the electron drift. From measurements of the plasma properties along the axis, it is inferred that the electron drift velocity is not uniform along the entire current channel. The evidence suggests that the waves begin being excited at that axial position where the critical drift velocity is first exceeded, consistent with a current-driven excitation mechanism.

Antifouling Transparent ZnO Thin Films Fabricated by Atmospheric Pressure Cold Plasma Deposition

NASA Astrophysics Data System (ADS)

Suzaki, Yoshifumi; Du, Jinlong; Yuji, Toshifumi; Miyagawa, Hayato; Ogawa, Kazufumi

2015-09-01

One problem with outdoor-mounted solar panels is that power generation efficiency is reduced by face plate dirt; a problem with electronic touch panels is the deterioration of screen visibility caused by finger grease stains. To solve these problems, we should fabricate antifouling surfaces which have superhydrophobic and oil-repellent properties without spoiling the transparency of the transparent substrate. In this study, an antifouling surface with both superhydrophobicity and oil-repellency was fabricated on a glass substrate by forming a fractal microstructure. The fractal microstructure was constituted of transparent silica particles 100 nm in diameter and transparent zinc-oxide columns grown on silica particles through atmospheric pressure cold plasma deposition; the sample surface was coated with a chemically adsorbed monomolecular layer. Samples were obtained which had a superhydrophobic property (with a water droplet contact angle of more than 150°) and a high average transmittance of about 90% (with wavelengths ranging from 400 nm to 780 nm).

Simultaneous quantification of soman and VX adducts to butyrylcholinesterase, their aged methylphosphonic acid adduct and butyrylcholinesterase in plasma using an off-column procainamide-gel separation method combined with UHPLC-MS/MS.

PubMed

Liu, Chang-Cai; Huang, Gui-Lan; Xi, Hai-Ling; Liu, Shi-Lei; Liu, Jing-Quan; Yu, Hui-Lan; Zhou, Shi-Kun; Liang, Long-Hui; Yuan, Ling

2016-11-15

This work describes a novel and sensitive non-isotope dilution method for simultaneous quantification of organophosphorus nerve agents (OPNAs) soman (GD) and VX adducts to butyrylcholinesterase (BChE), their aged methylphosphonic acid (MeP) adduct and unadducted BChE in plasma exposed to OPNA. OPNA-BChE adducts were isolated with an off-column procainamide-gel separation (PGS) from plasma, and then digested with pepsin into specific adducted FGES * AGAAS nonapeptide (NP) biomarkers. The resulting NPs were detected by UHPLC-MS/MS MRM. The off-column PGS method can capture over 90% of BChE, MeP-BChE, VX-BChE and GD-BChE from their respective plasma materials. One newly designed and easily synthesized phosphorylated BChE nonapeptide with one Gly-to-Ala mutation was successfully reported to serve as internal standard instead of traditional isotopically labeled BChE nonapeptide. The linear range of calibration curves were from 1.00-200ngmL -1 for VX-NP, 2.00-200ngmL -1 for GD-NP and MeP-NP (R 2 ≥0.995), and 3.00-200ngmL -1 for BChE NP (R 2 ≥0.990). The inter-day precision had relative standard deviation (%RSD) of <8.89%, and the accuracy ranged between 88.9-120%. The limit of detection was calculated to be 0.411, 0.750, 0.800 and 1.43ngmL -1 for VX-NP, GD-NP, MeP-NP and BChE NP, respectively. OPNA-exposed quality control plasma samples were characterized as part of method validation. Investigation of plasma samples unexposed to OPNA revealed no baseline values or interferences. Using the off-column PGS method combined with UHPLC-MS/MS, VX-NP and GD-NP adducts can be unambiguously detected with high confidence in 0.10ngmL -1 and 0.50ngmL -1 of exposed human plasma respectively, only requiring 0.1mL of plasma sample and taking about four hours without special sample preparation equipment. These improvements make it a simple, sensitive and robust PGS-UHPLC-MS/MS method, and this method will become an attractive alternative to immunomagnetic separation (IMS) method and a useful diagnostic tool for retrospective detection of OPNA exposure with high confidence. Furthermore, using the developed method, the adducted BChE levels from VX and GD-exposed (0.10-100ngmL -1 ) plasma samples were completely characterized, and the fact that VX being more active and specific to BChE than GD was re-confirmed. Copyright © 2016 Elsevier B.V. All rights reserved.

Classification of LC columns based on the QSRR method and selectivity toward moclobemide and its metabolites.

PubMed

Plenis, Alina; Olędzka, Ilona; Bączek, Tomasz

2013-05-05

This paper focuses on a comparative study of the column classification system based on the quantitative structure-retention relationships (QSRR method) and column performance in real biomedical analysis. The assay was carried out for the LC separation of moclobemide and its metabolites in human plasma, using a set of 24 stationary phases. The QSRR models established for the studied stationary phases were compared with the column test performance results under two chemometric techniques - the principal component analysis (PCA) and the hierarchical clustering analysis (HCA). The study confirmed that the stationary phase classes found closely related by the QSRR approach yielded comparable separation for moclobemide and its metabolites. Therefore, the QSRR method could be considered supportive in the selection of a suitable column for the biomedical analysis offering the selection of similar or dissimilar columns with a relatively higher certainty. Copyright © 2013 Elsevier B.V. All rights reserved.

Low-threshold parametric excitation of the upper hybrid wave in experiments on electron-cyclotron resonance heating by an ordinary wave

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sysoeva, E. V., E-mail: tinlit@yandex.ru; Gusakov, E. Z.; Simonchik, L. V.

2016-07-15

The possibility of the low-threshold decay of an ordinary wave into an upper hybrid wave localized in a plasma column (or in an axisymmetric plasma filament) and a low-frequency wave is analyzed. It is shown that the threshold for such a decay, accompanied by the excitation of an ion-acoustic wave, can easily be overcome for plasma parameters typical of model experiments on the Granit linear plasma facility.

Hybrid Sargassum-sand sorbent: a novel adsorbent in packed column to treat metal-bearing wastewaters from inductively coupled plasma-optical emission spectrometry.

PubMed

Vijayaraghavan, K; Joshi, U M

2013-01-01

Laboratory batch and column experiments were carried out to examine the efficiency of algal-based treatment technique to clean-up wastewaters emanating from inductively coupled plasma-optical emission spectrometry (ICP-OES). Chemical characterization revealed the extreme complexity of the wastewater, with the presence of 14 different metals under very low pH (pH = 1.1), high conductivity (6.98 mS/cm), total dissolved solid (4.46 g/L) and salinity (3.77). Batch experiments using Sargassum biomass indicated that it was possible to attain high removal efficiencies at optimum pH of 4.0. Efforts were also made to continuously treat ICP-OES wastewater using up-flow packed column. However, swelling of Sargassum biomass leads to stoppage of column. To address the problem, Sargassum was mixed with sand at a ratio of 40: 60 on volume basis. Remarkably, the hybrid Sargassum-sand sorbent showed very high removal efficiency towards multiple metal ions with the column able to operate for 11 h at a flow rate of 10 mL/min. Metal ions such as Cu, Cd, and Pb were only under trace levels in the treated water until 11 h. The results of the treatment process were compared with trade effluent discharge standards. Further the process evaluation and cost analysis were presented.

Kinetic description of cyclotron-range oscillations of a non-neutral plasma column

NASA Astrophysics Data System (ADS)

Neu, S. C.; Morales, G. J.

1998-04-01

The kinetic analysis introduced by Prasad, Morales, and Fried [Prasad et al., Phys. Fluids 30, 3093 (1987)] is used to derive damping conditions and a differential equation for azimuthally propagating waves in a non-neutral plasma column in the limits rl/L≪1 and krl≪1 (where rl is the Larmor radius, k is the wave number, and L is the density scale length). The predictions of the kinetic analysis are verified using a two-dimensional particle-in-cell simulation of Bernstein modes in a thermal rigid-rotor equilibrium. Differences between modes in a strongly magnetized limit and near the Brillouin limit are studied in the simulation.

A new restricted access molecularly imprinted polymer capped with albumin for direct extraction of drugs from biological matrices: the case of chlorpromazine in human plasma.

PubMed

de Oliveira Isac Moraes, Gabriel; da Silva, Larissa Meirelles Rodrigues; dos Santos-Neto, Alvaro José; Florenzano, Fábio Herbst; Figueiredo, Eduardo Costa

2013-09-01

A new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) was developed, characterized, and used for direct analysis of chlorpromazine in human plasma samples. The RAMIP-BSA was synthesized using chlorpromazine, methacrylic acid, and ethylene glycol dimethacrylate as template, functional monomer, and cross-linker, respectively. Glycerol dimethacrylate and hydroxy methyl methacrylate were used to promote a hydrophilic surface (high density of hydroxyl groups). Afterward, the polymer was coated with BSA using glutaraldehyde as cross-linker, resulting in a protein chemical shield around it. The material was able to eliminate ca. 99% of protein when a 44-mg mL(-1) BSA aqueous solution was passed through it. The RAMIP-BSA was packed in a column and used for direct analysis of chlorpromazine in human plasma samples in an online column switching high-performance liquid chromatography system. The analytical calibration curve was prepared in a pool of human plasma samples with chlorpromazine concentrations ranging from 30 to 350 μg L(-1). The correlation coefficient obtained was 0.995 and the limit of quantification was 30 μg L(-1). Intra-day and inter-day precision and accuracy presented variation coefficients and relative errors lower than 15% and within -15 and 15%, respectively. The sample throughput was 3 h(-1) (sample preparation and chromatographic analysis steps) and the same RAMIP-BSA column was efficiently used for about 90 cycles.

Broad ion energy distributions in helicon wave-coupled helium plasma

NASA Astrophysics Data System (ADS)

Woller, K. B.; Whyte, D. G.; Wright, G. M.

2017-05-01

Helium ion energy distributions were measured in helicon wave-coupled plasmas of the dynamics of ion implantation and sputtering of surface experiment using a retarding field energy analyzer. The shape of the energy distribution is a double-peak, characteristic of radiofrequency plasma potential modulation. The broad distribution is located within a radius of 0.8 cm, while the quartz tube of the plasma source has an inner radius of 2.2 cm. The ion energy distribution rapidly changes from a double-peak to a single peak in the radius range of 0.7-0.9 cm. The average ion energy is approximately uniform across the plasma column including the double-peak and single peak regions. The widths of the broad distribution, ΔE , in the wave-coupled mode are large compared to the time-averaged ion energy, ⟨E ⟩. On the axis (r = 0), ΔE / ⟨E ⟩ ≲ 3.4, and at a radius near the edge of the plasma column (r = 2.2 cm), ΔE / ⟨E ⟩ ˜ 1.2. The discharge parameter space is scanned to investigate the effects of the magnetic field, input power, and chamber fill pressure on the wave-coupled mode that exhibits the sharp radial variation in the ion energy distribution.

High power fast wave experiments in LAPD: interaction with density fluctuations and status/plans for ICRH

NASA Astrophysics Data System (ADS)

Carter, Troy; Martin, Michael; van Compernolle, Bart; Gekelman, Walter; Pribyl, Pat; Vincena, Stephen; Tripathi, Shreekrishna; van Eester, Dirk; Crombe, Kristel

2016-10-01

The LArge Plasma Device (LAPD) at UCLA is a 17 m long, up to 60 cm diameter magnetized plasma column with typical plasma parameters ne 1012 -1013 cm-3, Te 1 - 10 eV, and B 1 kG. A new high-power ( 200 kW) RF system and antenna has been developed for LAPD, enabling the generation of large amplitude fast waves in LAPD. Interaction between the fast waves and density fluctuations is observed, resulting in modulation of the coupled RF power. Two classes of RF-induced density fluctuations are observed. First, a coherent (10 kHz) oscillation is observed spatially near the antenna in response to the initial RF turn-on transient. Second, broadband density fluctuations are enhanced when the RF power is above a threshold a threshold. Strong modulation of the fast wave magnetic fluctuations is observed along with broadening of the primary RF spectral line. Ultimately, high power fast waves will be used for ion heating in LAPD through minority species fundamental heating or second harmonic minority or majority heating. Initial experimental results from heating experiments will be presented along with a discussion of future plans. BaPSF supported by NSF and DOE.

On the resonance amplification of magnetic perturbations near the threshold of tearing instability in a tokamak

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arsenin, V. V., E-mail: arsenin-vv@nrcki.ru; Skovoroda, A. A., E-mail: skovoroda-aa@nrcki.ru

2015-12-15

Using a cylindrical model, a relatively simple description is presented of how a magnetic field perturbation stimulated by a low external helical current or a small helical distortion of the boundary and generating magnetic islands penetrates into a plasma column with a magnetic surface q=m/n to which tearing instability is attached. Linear analysis of the classical instability with an aperiodic growth of the perturbation in time shows that the perturbation amplitude in plasma increases in a resonant manner as the discharge parameters approach the threshold of tearing instability. In a stationary case, under the assumption on the helical character ofmore » equilibrium, which can be found from the two-dimensional nonlinear equation for the helical flux, there is no requirement for the small size of the island. Examples of calculations in which magnetic islands are large near the threshold of tearing instability are presented. The bifurcation of equilibrium near the threshold of tearing instability in plasma with a cylindrical boundary, i.e., the existence of helical equilibrium (along with cylindrical equilibrium) with large islands, is described. Moreover, helical equilibrium can also exist in the absence of instability.« less

Development and Bioanalytical Validation of a Liquid Chromatographic-Tandem Mass Spectrometric (LC-MS/MS) Method for the Quantification of the CCR5 Antagonist Maraviroc in Human Plasma

PubMed Central

Emory, Joshua F.; Seserko, Lauren A.; Marzinke, Mark A.

2014-01-01

Background Maraviroc is a CCR5 antagonist that has been utilized as a viral entry inhibitor in the management of HIV-1. Current clinical trials are pursuing maraviroc drug efficacy in both oral and topical formulations. Therefore, in order to fully understand drug pharmacokinetics, a sensitive method is required to quantify plasma drug concentrations. Methods Maraviroc-spiked plasma was combined with acetonitrile containing an isotopically-labeled internal standard, and following protein precipitation, samples were evaporated to dryness and reconstituted for liquid chromatographic-tandem mass spectrometric (LC-MS/MS) analysis. Chromatographic separation was achieved on a Waters BEH C8, 50 × 2.1 mm UPLC column, with a 1.7 μm particle size and the eluent was analyzed using an API 4000 mass analyzer in selected reaction monitoring mode. The method was validated as per FDA Bioanalytical Method Validation guidelines. Results The analytical measuring range of the LC-MS/MS method is 0.5-1000 ng/ml. Calibration curves were generated using weighted 1/x2 quadratic regression. Inter-and intra-assay precision was ≤ 5.38% and ≤ 5.98%, respectively; inter-and intra-assay accuracy (%DEV) was ≤ 10.2% and ≤ 8.44%, respectively. Additional studies illustrated similar matrix effects between maraviroc and its internal standard, and that maraviroc is stable under a variety of conditions. Method comparison studies with a reference LC-MS/MS method show a slope of 0.948 with a Spearman coefficient of 0.98. Conclusions Based on the validation metrics, we have generated a sensitive and automated LC-MS/MS method for maraviroc quantification in human plasma. PMID:24561264

Regulated bioanalysis of conformers - A case study with ASP2151 in dog plasma and urine.

PubMed

Ohtsu, Yoshiaki; Otsuka, Shohei; Nakamura, Takeshi; Noguchi, Kiyoshi

2015-08-01

We developed and validated bioanalytical methods for a potent helicase-primase inhibitor ASP2151 that has two conformers. These conformers elute as unseparated broad peaks under ordinary high-performance liquid chromatographic conditions, indicating discernable differences in hydrophobicity. We observed that column temperature and mobile phase pH have no effect on these peaks and that conformers form a single symmetrical peak when tetrahydrofuran is added to the mobile phase. In addition, we needed to develop semi-automated methods where inter-conversion of the conformers is unlikely to cause sample-to-sample extraction variability. Briefly, following the addition of deuterium-labeled ASP2151 as an internal standard (IS), dog plasma samples or acetonitrile-added urine samples were filtrated. The filtrates were then injected into a column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) system and trapped onto an extraction column. Extracts were back-flushed onto an analytical C18 column (4.6×50mm, 3μm) with a mobile phase consisting of methanol, tetrahydrofuran, and 20mmol/L ammonium acetate (45:5:50, v/v/v). The eluent was monitored in the negative atmospheric pressure chemical ionization mode. The calibration curve was linear over a range of 5-1000ng/mL for plasma and 0.5-100μg/mL for urine. Validation data met the acceptance criteria in accordance with regulatory guidance and demonstrated that these methods were selective, accurate, and reproducible. In addition, the present methods were successfully applied to a pharmacokinetic study in dogs. Copyright © 2015 Elsevier B.V. All rights reserved.

Sensitive determination of four tetracycline antibiotics in pig plasma by field-amplified sample stacking open-tubular capillary electrochromatography with dimethylethanolamine aminated polychloromethyl styrene nano-latex coated capillary column.

PubMed

Guo, Yaxiao; Meng, Lei; Zhang, Yanhao; Tang, Wei; Zhang, Wenfen; Xia, Yan; Ban, Fuguo; Wu, Ningpeng; Zhang, Shusheng

2013-12-30

This paper described the preparation and application of a new dimethylethanolamine aminated polychloromethyl styrene nano-latex (DMEAPL) coated capillary column (ccc-DMEAPL) in the determination of four tetracycline antibiotics (TCA) including tetracycline (TC), oxytetracycline (OTC), doxycycline (DC) and chlorotetracycline (CTC) in pig plasma. The ccc-DMEAPL column was characterized with steady EOF values of ca. 1.5-5.2×10(-5)cm(2)/Vs at pH 1.8-6.3. The optimized conditions for field-amplified sample stacking open-tubular capillary electrochromatography (FASS-OT-CEC) were as following: background electrolyte, 10mmol/L Na2HPO4+15mmol/L citric acid (pH 3.2); ccc-DMEAPL, 50μm i.d.×50cm (effective length 41.5cm), separation voltage, 18kV; column temperature, 25°C; UV detection wavelength, 270nm; water-plug injection: 30mbar×10s; sample electrokinetic injection, 10kV×20s. The four TCA were extracted with the solution of 10mmol/L Na2HPO4+15mmol/L citric acid+4g/L EDTA-2Na (pH 3.2). The FASS-OT-CEC method was validated in terms of linearity, sensitivity, selectivity, precision and accuracy. The LODs ranged from 3 to 7ng/mL, the recoveries for the four TCA were all more than 80%. The developed method was successfully applied for the determination of TCs in the actual pig plasma samples. Copyright © 2013 Elsevier B.V. All rights reserved.

Characterization of fast deuterons involved in the production of fusion neutrons in a dense plasma focus

NASA Astrophysics Data System (ADS)

Kubes, P.; Paduch, M.; Sadowski, M. J.; Cikhardt, J.; Cikhardtova, B.; Klir, D.; Kravarik, J.; Munzar, V.; Rezac, K.; Zielinska, E.; Skladnik-Sadowska, E.; Szymaszek, A.; Tomaszewski, K.; Zaloga, D.

2018-01-01

This paper considers regions of a fast deuteron production in a correlation with an evolution of ordered structures inside a pinch column of a mega-ampere plasma focus discharge. Ion pinhole cameras equipped with plastic PM-355 track-detectors recorded fast deuterons escaping in the downstream and other directions (up to 60° to the z-axis). Time-integrated ion images made it possible to estimate sources of the deuteron acceleration at the known magnetic field and deuteron energy values. The images of the fast deuterons emitted in the solid angle ranging from 0° to 4° showed two forms: central spots and circular images. The spots of 1-2 cm in diameter were produced by deuterons from the central pinch regions. The circular-shaped images of a radius above 3 cm (or their parts) were formed by deuterons from the region surrounding the dense pinch column. The ion pinhole cameras placed at angles above 20° to the z-axis recorded the ion spots only, and the ring-images were missing. The central region of the deuteron acceleration could be associated mainly with plasmoids, and the circular images could be connected with ring-shaped regions of the radius corresponding to tops of the plasma lobules outside the dense pinch column. The deuteron tracks forming ring-shaped images of a smaller (0.5-1) cm radius could be produced by deflections of the fast deuterons, which were caused by a magnetic field inside the dense pinch column.

Heating and cooling of the multiply charged ion nonequilibrium plasma in a high-current extended low-inductance discharge

NASA Astrophysics Data System (ADS)

Burtsev, V. A.; Kalinin, N. V.

2014-09-01

Using a radiation magnetohydrodynamics two-temperature model (RMHD model) of a high-current volumetric radiating Z-discharge, the heating and cooling of the nitrogen plasma in a pulsed pinched extended discharge is investigated as applied to the problem of creating a recombination laser based on 3 → 2 transitions of hydrogen-like nitrogen ions (λ = 13.4 nm). It is shown that the power supply of the discharge, which is represented by a dual storage-forming line and a transmission line, makes it possible to raise the power density of the nitrogen plasma to 0.01-1.00 TW/cm3. Accordingly, there arises the possibility of generating a fully ionized (i.e., consisting of bare nuclei and electrons) plasma through the heating (compression) of electrons owing to the self-magnetic field of the plasma current and Joule heat even if the plasma is cooled by its own radiation at this stage. Such a plasma is needed to produce the lasing (active) medium of a recombination laser based on electron transitions in hydrogen-like ions. At the second stage, it is necessary to rapidly and deeply cool the plasma to 20-40 eV for 1-2 ns. Cooling of the fully ionized expanding plasma was numerically simulated with the discharge current switched on and off by means of a switch with a rapidly rising resistance. In both cases, the plasma expansion in the discharge is not adiabatic. Even after the discharge current is fairly rapidly switched off, heating of electrons continues inside the plasma column for a time longer than the switching time. Discharge current switchoff improves the electron cooling efficiency only slightly. Under such conditions, the plasma cools down to 50-60 eV in the former case and to 46-54 eV in the latter case for 2-3 ns.

SPQR II: A beam-plasma interaction experiment

NASA Astrophysics Data System (ADS)

Bimbot, R.; Della-Negra, S.; Gardès, D.; Rivet, M. F.; Fleurier, C.; Dumax, B.; Hoffman, D. H. H.; Weyrich, K.; Deutsch, C.; Maynard, G.

1986-01-01

SPQR II is an interaction experiment designed to probe energy -and charge-exchange of Cn+ ions at 2 MeV/a.m.u., flowing through a fully ionized plasma column of hydrogen with nℓ=1019 e-cm-2 at T=5 eV. One expects a factor of two enhanced stopping over the cold gas case.

Effects of Io ejecta on Europa

NASA Astrophysics Data System (ADS)

Eviatar, A.; Siscoe, G. L.; Johnson, T. V.; Matson, D. L.

1981-07-01

The effects of plasma ejected from Io on the nature and evolution of the surface of Europa and on the relative importance of the roles played by the two satellites in the Jupiter magnetosphere are examined. Observations of an ultraviolet absorption feature on the trailing side of Europa are interpreted as due to an equilibrium column density of SO2 in a steady-state model of the implantation of iogenic ions into the surface of Europa and their subsequent sputtering. The observed sulfur column density of 2 x 10 to the 16th/sq cm implies a slow loss of material from Europa, mainly water ice, and indicates that the spectrum of particles sputtered is soft. Considerations of the comparative roles of corotating and energetic heavy ions are shown to suggest that the implantation and sputtering is primarily the result of the proton and light ion component of the plasma. The weakness of Europa as a plasma source resulting from the soft sputtered particle spectrum thus leads to the dominance of Io in contributing to the magnetospheric plasma.

Isoflavone-free soy protein prepared by column chromatography reduces plasma cholesterol in rats.

PubMed

Fukui, Kensuke; Tachibana, Nobuhiko; Wanezaki, Satoshi; Tsuzaki, Shinichi; Takamatsu, Kiyoharu; Yamamoto, Takashi; Hashimoto, Yukio; Shimoda, Tadahisa

2002-09-25

To know whether isoflavones are responsible for the hypocholesterolemic effect of soy protein, the effect on plasma cholesterol of isoflavone-free soy protein prepared by column chromatography was examined in rats. Five-week-old male Sprague-Dawley rats were fed cholesterol-enriched AIN-93G diets containing either 20% casein (CAS), 20% soy protein isolate (SPI), 20% isoflavone-free SPI (IF-SPI), 19.7% IF-SPI + 0.3% isoflavone-rich fraction (isoflavone concentrate, IC), or 20% CAS + 0.3% IC for 2 weeks. Plasma total cholesterol concentrations of rats fed SPI and IF-SPI were comparable and were significantly lower than that of rats fed CAS. The addition of IC to the CAS and IF-SPI did not influence plasma cholesterol level. Fecal steroid excretion of the three SPI groups was higher than that of the two CAS groups, whereas the addition of IC showed no effect. Thus, a significant fraction of the cholesterol-lowering effect of SPI in rats can be attributed to the protein content, but the isoflavones and other minor constituents may also play a role.

Direct-injection HPLC method of measuring micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column.

PubMed

Uranishi, Hiroaki; Nakamura, Mitsuhiro; Nakamura, Hiroki; Ikeda, Yukari; Otsuka, Mayuko; Kato, Zenichiro; Tsuchiya, Teruo

2011-04-15

A direct-injection HPLC-based method has been developed for determining amounts of micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column. The method is easy to perform and requires only 10 μL of a filtered plasma sample. The chromatographic separations were carried out with a gradient mode. The fluorescence detection wavelengths of excitation and emission were set at 273 nm and 464 nm, respectively. Retention times for micafungin and IS were 22.4 and 23.7 min, respectively. Micafungin and FR195743 (IS) peaks were completely separated with little tailing, and no interference was observed. The calibration curve of micafungin showed good linearity in the range of 0.5-20.0 μg/mL (r(2)=1.00). The intra-day accuracy ranged from -4.5 to 5.3%. The inter-day accuracy ranged from -9.8 to 1.5%. The precisions were less than 10%. This method is useful for the determination of micafungin in human plasma. Copyright © 2011 Elsevier B.V. All rights reserved.

Sensitive Detection of α-Conotoxin GI in Human Plasma Using a Solid-Phase Extraction Column and LC-MS/MS.

PubMed

Yu, Shuo; Yang, Bo; Yan, Liangping; Dai, Qiuyun

2017-07-28

α-conotoxin GI, a short peptide toxin in the venom of Conus geographus , is composed of 13 amino acids and two disulfide bonds. It is the most toxic component of Conus geographus venom with estimated lethal doses of 0.029-0.038 mg/kg for humans. There is currently no reported analytical method for this toxin. In the present study, a sensitive detection method was developed to quantify GI in human plasma using a solid-phase extraction (SPE) column (polystyrene-divinyl benzene copolymer) combined with liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in the multiple reaction monitoring (MRM) mode. The plasma samples were treated with a protein precipitating solvent (methanol: acetonitrile = 50:50, v / v ). GI in the solvent was efficiently extracted with an SPE column and was further separated by a Grace Alltima HP C 18 (50 × 2.1 mm, 5 μm) column at a flow rate of 0.4 mL/min. Water (with 2% methanol) acetonitrile (with 0.1% acetic acid) was selected as the mobile phase combination used in a linear gradient system. α-Conotoxin GI was analyzed by an API 4000 triple quadrupole mass spectrometer. In the method validation, the linear calibration curve in the range of 2.0 to 300.0 ng/mL had correlation coefficients ( r ) above 0.996. The recovery was 57.6-66.8% for GI and the internal standard. The lower limit of quantification (LLOQ) was 2 ng/mL. The intra- and inter-batch precisions were below 6.31% and 8.61%, respectively, and the accuracies were all within acceptance. GI was stable in a bench-top autosampler through long-term storage and freeze/thaw cycles. Therefore, this method is specific, sensitive and reliable for quantitative analysis of α-conotoxin GI in human plasma.

Bilayer Suspension Plasma-Sprayed Thermal Barrier Coatings with Enhanced Thermal Cyclic Lifetime: Experiments and Modeling

NASA Astrophysics Data System (ADS)

Gupta, Mohit; Kumara, Chamara; Nylén, Per

2017-08-01

Suspension plasma spraying (SPS) has been shown as a promising process to produce porous columnar strain tolerant coatings for thermal barrier coatings (TBCs) in gas turbine engines. However, the highly porous structure is vulnerable to crack propagation, especially near the topcoat-bondcoat interface where high stresses are generated due to thermal cycling. A topcoat layer with high toughness near the topcoat-bondcoat interface could be beneficial to enhance thermal cyclic lifetime of SPS TBCs. In this work, a bilayer coating system consisting of first a dense layer near the topcoat-bondcoat interface followed by a porous columnar layer was fabricated by SPS using Yttria-stabilised zirconia suspension. The objective of this work was to investigate if the bilayer topcoat architecture could enhance the thermal cyclic lifetime of SPS TBCs through experiments and to understand the effect of the column gaps/vertical cracks and the dense layer on the generated stresses in the TBC during thermal cyclic loading through finite element modeling. The experimental results show that the bilayer TBC had significantly higher lifetime than the single-layer TBC. The modeling results show that the dense layer and vertical cracks are beneficial as they reduce the thermally induced stresses which thus increase the lifetime.

Microwave Argon Plasma Torch

DTIC Science & Technology

2013-07-01

31st ICPIG, July 14-19, 2013, Granada , Spain Microwave Argon plasma torch E. Benova1, M. Pencheva-Atanasova1,2, P. Marinova1, V. Marchev1, T...See also ADA594770. International Conference on Phenomena in Ionized Gases (31st) (ICPIG) Held in Granada , Spain on 14-19 July 2013, The original...18 31st ICPIG, July 14-19, 2013, Granada , Spain EEDF usually strongly differs from Maxwellian and chages along the plasma column (this is

Generator for ionic gallium-68 based on column chromatography

DOEpatents

Neirinckx, Rudi D.; Davis, Michael A.

1981-01-01

A physiologically acceptable solution of gallium-68 fluorides, having an activity of 0.1 to 50 millicuries per milliliter of solution is provided. The solution is obtained from a generator comprising germanium-68 hexafluoride bound to a column of an anion exchange resin which forms gallium-68 in situ by eluting the column with an acid solution to form a solution containing .sup.68 Ga-fluorides. The solution then is neutralized prior to administration.

Simultaneous and rapid determination of gefitinib, erlotinib and afatinib plasma levels using liquid chromatography/tandem mass spectrometry in patients with non-small-cell lung cancer.

PubMed

Hayashi, Hideki; Kita, Yutaro; Iihara, Hirotoshi; Yanase, Koumei; Ohno, Yasushi; Hirose, Chiemi; Yamada, Maya; Todoroki, Kenichiro; Kitaichi, Kiyoyuki; Minatoguchi, Shinya; Itoh, Yoshinori; Sugiyama, Tadashi

2016-07-01

A simultaneous, selective, sensitive and rapid liquid chromatography/tandem mass spectrometry method was developed and validated for the quantification of gefitinib, erlotinib and afatinib in 250 μL samples of human blood plasma. Diluted plasma samples were extracted using a liquid-phase extraction procedure with tert-butyl methyl ether. The three drugs were separated by high-performance liquid chromatography using a C18 column and an isocratic mobile phase running at a flow rate of 0.2 mL/min for 5 min. The drugs were detected using a tandem mass spectrometer with electrospray ionization using imatinib as an internal standard. Calibration curves were generated over the linear concentration range of 0.05-100 nm in plasma with a lower limit of quantification of 0.01 or 0.05 nm for all compounds. Finally, the validated method was applied to a clinical pharmacokinetic study in patients with nonsmall-cell lung cancer (NSCLC) following the oral administration of afatinib. These results indicate that this method is suitable for assessing the risks and benefits of chemotherapy in patients with NSCLC and is useful for therapeutic drug monitoring for NSCLC treatment. As far as we know, this is the first report on LC-MS/MS method for the simultaneous quantification of NSCLC tyrosine kinase inhibitor plasma concentrations including afatinib. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

PALS laser-driven radiative jets for astrophysical and ICF applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pisarczyk, T.; Kasperczuk, A.; Stenz, Ch.

2008-03-19

High speed, well-collimated plasma jets were generated in the interaction of defocused single laser beam with planar, massive Cu target. The experiment was carried out at the iodine laser facility (Prague Asterix Laser System--PALS) using the third harmonic beam (0.438 {mu}m) with a pulse duration of 250 ps (FWHM) and an energy of 100 J. The information about geometry of plasma expansion, plasma dynamics and electron density were obtained by means of a 3-frame interferometric system. The plasma jet parameters reach the following values: the velocity up to 7x10{sup 7} cm/s, the internal Mach number greater than 10 and themore » electron density above 10{sup 19} cm{sup -3}. The jet characteristics are appropriate for the astrophysical and ICF applications. To ensure the interaction of this jet with gas or plasma as an ambient medium, a high-pressure supersonic gas nozzle was used, which created a cylindrical column of Ar or He. The results of first experiments dedicated to studies of collision of such a jet with a gas cloud are also presented. They clearly show the effect of shocks formation in ambient gases (He and Ar) due to the jet action. In the case of He the shock waves have usually a conical shape with a thickness of 1-1.5 mm, whereas in the case of Ar, the shock wave configuration is more complex and its thickness is less than 1 mm.« less

Laser ablation and column formation in silicon under oxygen-rich atmospheres

NASA Astrophysics Data System (ADS)

Pedraza, A. J.; Fowlkes, J. D.; Lowndes, D. H.

2000-11-01

The microstructure formed at the surface of silicon by cumulative pulsed-laser irradiation in oxygen-rich atmospheres consists of an array of microcolumns surrounded by microcanyons and microholes. Formation of SiOx at the exposed surface of silicon is most likely responsible for the occurrence of etching/ablation that causes the continuous deepening of canyons and holes. The growth mechanism of columns that is supported by the experimental evidence presented here is a process in which the columns are fed at their tips by the silicon-rich ablation plasma produced during pulsed-laser irradiation.

A microfluidic device for preparing next generation DNA sequencing libraries and for automating other laboratory protocols that require one or more column chromatography steps.

PubMed

Tan, Swee Jin; Phan, Huan; Gerry, Benjamin Michael; Kuhn, Alexandre; Hong, Lewis Zuocheng; Min Ong, Yao; Poon, Polly Suk Yean; Unger, Marc Alexander; Jones, Robert C; Quake, Stephen R; Burkholder, William F

2013-01-01

Library preparation for next-generation DNA sequencing (NGS) remains a key bottleneck in the sequencing process which can be relieved through improved automation and miniaturization. We describe a microfluidic device for automating laboratory protocols that require one or more column chromatography steps and demonstrate its utility for preparing Next Generation sequencing libraries for the Illumina and Ion Torrent platforms. Sixteen different libraries can be generated simultaneously with significantly reduced reagent cost and hands-on time compared to manual library preparation. Using an appropriate column matrix and buffers, size selection can be performed on-chip following end-repair, dA tailing, and linker ligation, so that the libraries eluted from the chip are ready for sequencing. The core architecture of the device ensures uniform, reproducible column packing without user supervision and accommodates multiple routine protocol steps in any sequence, such as reagent mixing and incubation; column packing, loading, washing, elution, and regeneration; capture of eluted material for use as a substrate in a later step of the protocol; and removal of one column matrix so that two or more column matrices with different functional properties can be used in the same protocol. The microfluidic device is mounted on a plastic carrier so that reagents and products can be aliquoted and recovered using standard pipettors and liquid handling robots. The carrier-mounted device is operated using a benchtop controller that seals and operates the device with programmable temperature control, eliminating any requirement for the user to manually attach tubing or connectors. In addition to NGS library preparation, the device and controller are suitable for automating other time-consuming and error-prone laboratory protocols requiring column chromatography steps, such as chromatin immunoprecipitation.

A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

PubMed Central

Tan, Swee Jin; Phan, Huan; Gerry, Benjamin Michael; Kuhn, Alexandre; Hong, Lewis Zuocheng; Min Ong, Yao; Poon, Polly Suk Yean; Unger, Marc Alexander; Jones, Robert C.; Quake, Stephen R.; Burkholder, William F.

2013-01-01

Library preparation for next-generation DNA sequencing (NGS) remains a key bottleneck in the sequencing process which can be relieved through improved automation and miniaturization. We describe a microfluidic device for automating laboratory protocols that require one or more column chromatography steps and demonstrate its utility for preparing Next Generation sequencing libraries for the Illumina and Ion Torrent platforms. Sixteen different libraries can be generated simultaneously with significantly reduced reagent cost and hands-on time compared to manual library preparation. Using an appropriate column matrix and buffers, size selection can be performed on-chip following end-repair, dA tailing, and linker ligation, so that the libraries eluted from the chip are ready for sequencing. The core architecture of the device ensures uniform, reproducible column packing without user supervision and accommodates multiple routine protocol steps in any sequence, such as reagent mixing and incubation; column packing, loading, washing, elution, and regeneration; capture of eluted material for use as a substrate in a later step of the protocol; and removal of one column matrix so that two or more column matrices with different functional properties can be used in the same protocol. The microfluidic device is mounted on a plastic carrier so that reagents and products can be aliquoted and recovered using standard pipettors and liquid handling robots. The carrier-mounted device is operated using a benchtop controller that seals and operates the device with programmable temperature control, eliminating any requirement for the user to manually attach tubing or connectors. In addition to NGS library preparation, the device and controller are suitable for automating other time-consuming and error-prone laboratory protocols requiring column chromatography steps, such as chromatin immunoprecipitation. PMID:23894273

Casingless down-hole for sealing an ablation volume and obtaining a sample for analysis

DOEpatents

Noble, Donald T.; Braymen, Steven D.; Anderson, Marvin S.

1996-10-01

A casing-less down hole sampling system for acquiring a subsurface sample for analysis using an inductively coupled plasma system is disclosed. The system includes a probe which is pushed into the formation to be analyzed using a hydraulic ram system. The probe includes a detachable tip member which has a soil point mad a barb, with the soil point aiding the penetration of the earth, and the barb causing the tip member to disengage from the probe and remain in the formation when the probe is pulled up. The probe is forced into the formation to be tested, and then pulled up slightly, to disengage the tip member and expose a column of the subsurface formation to be tested. An instrumentation tube mounted in the probe is then extended outward from the probe to longitudinally extend into the exposed column. A balloon seal mounted on the end of the instrumentation tube allows the bottom of the column to be sealed. A source of laser radiation is emitted from the instrumentation tube to ablate a sample from the exposed column. The instrumentation tube can be rotated in the probe to sweep the laser source across the surface of the exposed column. An aerosol transport system carries the ablated sample from the probe to the surface for testing in an inductively coupled plasma system. By testing at various levels in the down-hole as the probe is extracted from the soil, a profile of the subsurface formation may be obtained.

Analysis of essential oils and fragrances with a new generation of highly inert gas chromatographic columns coated with ionic liquids.

PubMed

Cagliero, Cecilia; Bicchi, Carlo; Cordero, Chiara; Liberto, Erica; Rubiolo, Patrizia; Sgorbini, Barbara

2017-04-28

In the fields of essential oils and fragrances, samples often consist of mixtures of compounds with similar structural and physical characteristics (e.g. mono- and sesquiterpenoids), whose correct identification closely depends on the synergic combination of chromatographic and mass spectral data. This sample complexity means that new GC stationary phases with different selectivities are continually being investigated. Ionic liquids (ILs) are of great interest as GC stationary phases in this field because of their selectivity (significantly different than that of currently phases) and their high temperature stability. A first generation of IL GC columns was found to be competitive when applied to these field, in terms of selectivity and efficiency, compared to conventional columns (polydimethylsiloxane, (e.g. OV-1), methyl-polysiloxane 5%-phenyl (e.g. SE-52), 7%-cyanopropyl, 7%-phenyl polysiloxane (e.g. OV-1701), and polyethylen glycol (e.g. PEG-20M). However, these columns showed significant activity towards polar or active analytes, which primarily affected their quantitative performance. A new generation of highly-inactive columns coated with three of the most widely-used ionic liquid GC stationary phases has recently been introduced; these phases are SLB-IL60i (1,12-di(tripropylphosphonium) dodecane bis(trifluoromethylsulfonyl) imide [NTf 2 ], SLB-IL76i (tri-(tripropylphosphonium-hexanamido)-triethylamine [NTf 2 ]), and SLB-IL111i (1,5-di (2,3-dimethyllimidazolium) pentane [NTf 2 ]). This study carefully tested the new inert IL columns, in view of their routine application in the fragrance and essential oil fields. They were found to have unusually high selectivity, comparable to that of first-generation IL columns, while their inertness and efficiency were competitive with those of currently-used conventional columns. The IL column performance of first and second generations was compared, through the quali-quantitative analysis of components in a group of different complexity samples; these included the Grob test, a standard mixture of "suspected" skin allergens, and the essential oils of chamomile and sandalwood. Copyright © 2017 Elsevier B.V. All rights reserved.

Radially localized helicon modes in nonuniform plasma

PubMed

Breizman; Arefiev

2000-04-24

A radial density gradient in an axisymmetric cylindrical plasma column forms a potential well for nonaxisymmetric helicon modes ( m not equal0). This paper presents an analytic description of such modes in the limit of small longitudinal wave numbers. The corresponding mode equation indicates the possibility of efficient resonant absorption of rf power in helicon discharges at unusually low frequencies.

Foaming in chemical surfactant free aqueous dispersions of anatase (titanium dioxide) particles.

PubMed

Pugh, R J

2007-07-17

Steady-state dynamic aqueous foams were generated from surfactant-free dispersion of aggregated anatase nanoparticles (in the micrometer size range). In order to tune the particle surfaces, to ensure a critical degree of hydrophobicity (so that they disperse in water and generate foam), the particles were subjected to low-temperature plasma treatment in the presence of a vapor-phase silane coupling agents. From ESCA it was shown that hydrophobization only occurred at a small number of surface sites. Foamability (foam generation) experiments were carried out under well-defined conditions at a range of gas flow rates using the Bikermann Foaming Column.1 The volume of the steady-state foams was determined under constant gas flow conditions, but on removing the gas flow, transient foams with short decay times (<5 s) were observed. The foamability of the steady-state foams was found to be dependent on (a) the time of plasma treatment of the particles (surface hydrophobicity), (b) the particle concentration in the suspension, and (c) the state of dispersion of the particles. High foamability was promoted in the neutral pH regions where the charged particles were highly dispersed. In the low and high pH regions where the particles were coagulated, the foamability was considerably reduced. This behavior was explained by the fact that the large coagula were less easily captured by the bubbles and more easily detached from the interface (during the turbulent foaming conditions) than individual dispersed particles.

Space-plasma campaign on UCLA's Large Plasma Device (LAPD)

NASA Astrophysics Data System (ADS)

Koepke, M. E.; Finnegan, S. M.; Knudsen, D. J.; Vincena, S.

2007-05-01

Knudsen [JGR, 1996] describes a potential role for stationary Alfvén (StA) waves in auroral arcs' frequency dependence. Magnetized plasmas are predicted to support electromagnetic perturbations that are static in a fixed frame if there is uniform background plasma convection. These stationary waves should not be confused with standing waves that oscillate in time with a fixed, spatially varying envelope. Stationary waves have no time variation in the fixed frame. In the drifting frame, there is an apparent time dependence as plasma convects past fixed electromagnetic structures. We describe early results from an experimental campaign to reproduce in the lab the basic conditions necessary for the creation of StA waves, namely quasi-steady-state convection across magnetic field-aligned current channels. We show that an off-axis, fixed channel of electron current (and depleted density) is created in the Large Plasma Device Upgrade (LAPD) at UCLA, using a small, heated, oxide-coated electrode at one plasma-column end and we show that the larger plasma column rotates about its cylindrical axis from a radial electric field imposed by a special termination electrode on the same end. Initial experimentation with plasma-rotation-inducing termination electrodes began in May 2006 in the West Virginia Q Machine, leading to two designs that, in January 2007, were tested in LAPD. The radial profile of azimuthal velocity was consistent with predictions of rigid-body rotation. Current-channel experiments in LAPD, in August 2006, showed that inertial Alfvén waves could be concentrated in an off-axis channel of electron current and depleted plasma density. These experimental results will be presented and discussed. This research is supported by DOE and NSF.

Characterization of the column and autocellular junctions that define the vasculature of gill lamellae.

PubMed

Kato, Akira; Nakamura, Korefumi; Kudo, Hisayuki; Tran, Yen Ha; Yamamoto, Yoko; Doi, Hiroyuki; Hirose, Shigehisa

2007-09-01

Novel adhesion junctions have been characterized that are formed at the interface between pillar cells and collagen columns, both of which are essential constituents of the gill lamellae in fish. We termed these junctions the "column junction" and "autocellular junction" and determined their molecular compositions by immunofluorescence microscopy using pufferfish. We visualized collagen columns by concanavalin A staining and found that the components of integrin-mediated cell-matrix adhesion, such as talin, vinculin, paxillin, and fibronectin, were concentrated on plasma membranes surrounding collagen columns (column membranes). This connection is analogous to the focal adhesion of cultured mammalian cells, dense plaque of smooth muscle cells, and myotendinous junction of skeletal muscle cells. We named this connection the "column junction." In the cytoplasm near the column, actin fibers, actinin, and a phosphorylated myosin light chain of 20 kDa are densely located, suggesting the contractile nature of pillar cells. The membrane infoldings surrounding the collagen columns were found to be connected by the autocellular junction, whose components are highly tyrosine-phosphorylated and contain the tight junction protein ZO-1. This study represents the first molecular characterization and fluorescence visualization of the column and autocellular junctions involved in both maintaining structural integrity and the hemodynamics of the branchial lamellae.

Exploring the Diffuse X-ray Emission of Supernova Remnant Kesteven 69 with XMM-Newton

NASA Astrophysics Data System (ADS)

Seo, Kyoung-Ae; Hui, Chung Yue

2013-06-01

We have investigated the X-ray emission from the shock-heated plasma of the Galactic supernova remnant Kesteven 69 with XMM-Newton. Assuming the plasma is at collisional ionization equilibrium, a plasma temperature and a column absorption are found to be kT ~ 0.62 keV and NH ~ 2.85 ×10^22 cm-2 respectively by imaging spectroscopy. Together with the deduced emission measure, we place constraints on its Sedov parameters.

The determination of fenspiride in human plasma and urine by liquid chromatography with electrochemical or ultraviolet detection.

PubMed

Sauveur, C; Baune, A; Vergnes, N; Jeanniot, J P

1989-01-01

A selective and sensitive method for the determination of fenspiride in biological fluids is described. The method involves liquid-liquid extraction followed by separation on a reversed-phase column with electrochemical detection for low levels of the drug in plasma (less than or equal to 100 ng ml-1) or UV absorption for higher concentrations in plasma or urine. The method is suitable for pharmacokinetic analyses and drug monitoring studies.

Neutron camera employing row and column summations

DOEpatents

Clonts, Lloyd G.; Diawara, Yacouba; Donahue, Jr, Cornelius; Montcalm, Christopher A.; Riedel, Richard A.; Visscher, Theodore

2016-06-14

For each photomultiplier tube in an Anger camera, an R.times.S array of preamplifiers is provided to detect electrons generated within the photomultiplier tube. The outputs of the preamplifiers are digitized to measure the magnitude of the signals from each preamplifier. For each photomultiplier tube, a corresponding summation circuitry including R row summation circuits and S column summation circuits numerically add the magnitudes of the signals from preamplifiers for each row and for each column to generate histograms. For a P.times.Q array of photomultiplier tubes, P.times.Q summation circuitries generate P.times.Q row histograms including R entries and P.times.Q column histograms including S entries. The total set of histograms include P.times.Q.times.(R+S) entries, which can be analyzed by a position calculation circuit to determine the locations of events (detection of a neutron).

Toroidal band limiter for a plasma containment device

DOEpatents

Kelley, George G.

1978-01-01

This invention relates to a toroidal plasma confinement device having poloidal and toroidal magnetic fields for confining a toroidal plasma column with a plasma current induced therein along an endless, circular equilibrium axis in a torus vacuum cavity wherein the improvement comprises the use of a toroidal plasma band limiter mounted within the vacuum cavity in such a manner as to ensure that the plasma energy is distributed more uniformly over the limiter surface thereby avoiding intense local heating of the limiter while at the same time substantially preventing damage to the plasma containment wall of the cavity by the energetic particles diffusing out from the confined plasma. A plurality of poloidal plasma ring limiters are also utilized for containment wall protection during any disruptive instability that might occur during operation of the device.

Development of a highly sensitive methodology for quantitative determination of fexofenadine in a microdose study by multiple injection method using ultra-high performance liquid chromatography with tandem mass spectrometry.

PubMed

Tanaka, Yukari; Yoshikawa, Yutaka; Yasui, Hiroyuki

2012-01-01

An ultra high-sensitivity method for quantifying fexofenadine concentration in rat plasma samples by multiple injection method (MIM) was developed for a microdose study. In this study, MIM involved continuous injections of multiple samples containing the single compound into a column of the ultra-HPLC (UHPLC) system, and then, temporary trapping of the analyte at the column head. This was followed by elution of the compound from the column and detection by mass spectrometer. Fexofenadine, used as a model compound in this study, was extracted from the plasma samples by a protein precipitation method. Chromatographic separation was achieved on a reversed-phase C18 column by using a gradient method with 0.1% formic acid and 0.1% formic acid in acetonitrile as the mobile phase. The analyte was quantified in the positive-ion electrospray ionization mode using selected reaction monitoring. In this study, the analytical time per fexofenadine sample was approximately 2 min according to the UHPLC system. The method exhibited the linear dynamic ranges of 5-5000 pg/mL for fexofenadine in rat plasma. The intra-day precisions were from 3.2 to 8.7% and the accuracy range was 95.2-99.3%. The inter-day precisions and accuracies ranged from 3.5 to 8.4% and from 98.6 to 102.6%, respectively. The validated MIM was successfully applied to a microdose study in the rats that received oral administration of 100 µg/kg fexofenadine. We suggest that this method might be beneficial for the quantification of fexofenadine concentrations in a microdose clinical study.

Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with UV-Vis detection.

PubMed

Cetin, Sevil Müge; Atmaca, Sedef

2004-03-26

A simple and reliable high-performance liquid chromatographic (HPLC) method with UV-Vis detection has been developed and validated for the determination of vigabatrin (VG) in human plasma and urine. The samples were pre-column derivatizated with 1,2-naphthoquinone-4-sulphonic acid sodium salt (NQS). A good chromatographic separation was achieved on a C18 column with a mobile phase consisting of acetonitrile and 10 mM orthophosphoric acid (pH 2.5) gradient elution. Tranexamic acid was used as an internal standard (I.S.). The method was linear over the concentration range of 0.8-30.0 microg/ml for both samples. The method is precise (relative standard deviation (R.S.D.) <9.13%) and accurate (relative mean error (RME) <-8.75%); analytical recoveries were 81.07% for plasma and 83.05% for urine. The assay was applied to pharmacokinetic study in a healthy volunteer after a single oral administration of 1 g of vigabatrin.

Finite-Length Diocotron Modes in a Non-neutral Plasma Column

NASA Astrophysics Data System (ADS)

Walsh, Daniel; Dubin, Daniel

2017-10-01

Diocotron modes are 2D distortions of a non-neutral plasma column that propagate azimuthally via E × B drifts. While the infinite-length theory of diocotron modes is well-understood for arbitrary azimuthal mode number l, the finite-length mode frequency is less developed (with some exceptions), and is naturally of relevance to experiments. In this poster, we present an approach to address finite length effects, such as temperature dependence of the mode frequency. We use a bounce-averaged solution to the Vlasov Equation, in which the Vlasov Equation is solved using action-angle variables of the unperturbed Hamiltonian. We write the distribution function as a Fourier series in the bounce-angle variable ψ, keeping only the bounce-averaged term. We demonstrate a numerical solution to this equation for a realistic plasma with a finite Debye Length, compare to the existing l = 1 theory, and discuss possible extensions of the existing theory to l ≠ 1 . Supported by NSF/DOE Partnership Grants PHY1414570 and DESC0002451.

Water-compatible molecularly imprinted polymers for efficient direct injection on-line solid-phase extraction of ropivacaine and bupivacaine from human plasma.

PubMed

Cobb, Zoe; Sellergren, Börje; Andersson, Lars I

2007-12-01

Two novel molecularly imprinted polymers (MIPs) selected from a combinatorial library of bupivacaine imprinted polymers were used for selective on-line solid-phase extraction of bupivacaine and ropivacaine from human plasma. The MIPs were prepared using methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linking monomer and in addition hydroxyethylmethacrylate to render the polymer surface hydrophilic. The novel MIPs showed high selectivity for the analytes and required fewer and lower concentrations of additives to suppress non-specific adsorption compared with a conventional MIP. This enabled the development of an on-line system for direct extraction of buffered plasma. Selective extraction was achieved without the use of time-consuming solvent switch steps, and transfer of the analytes from the MIP column to the analytical column was carried out under aqueous conditions fully compatible with reversed-phase LC gradient separation of analyte and internal standard. The MIPs showed excellent aqueous compatibility and yielded extractions with acceptable recovery and high selectivity.

Interactions of lectins with plasma membrane glycoproteins of the Ehrlich ascites carcinoma cell.

PubMed

Nachbar, M S; Oppenheim, J D; Aull, F

1976-02-06

Several aspects of the interaction of various lectins with the surface of Ehrlich ascites carcinoma cells are described. The order of agglutinating activity for various lectins is Ricinus communis greater than wheat germ greater than or equal to concanavalin A greater than or equal to soybean greater than Limulus polyphemus. No agglutination was noted for Ulex europaeus. Using 125I-labeled lectins it was determined that there are 1.6 and 7 times as many Ricinus communis lectin binding sites for concanavalin A and soybean lectins. Sodium deoxycholate-solubilized plasma membrane material was subjected to lectin affinity chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The lectin receptors of the plasma membrane appeared to be heterogeneous and some qualitative differences could be discerned among the electrophoretically analyzed material, which bound to and was specifically eluted from the various lectin affinity columns. The characteristics of elution of bound material from individual lectin columns indicated secondary hydrophobic interactions between concanavalin A or wheat germ agglutinin and their respective lectin receptor molecules.

A spin column-free approach to sodium hydroxide-based glycan permethylation.

PubMed

Hu, Yueming; Borges, Chad R

2017-07-24

Glycan permethylation was introduced as a tool to facilitate the study of glycans in 1903. Since that time, permethylation procedures have been continually modified to improve permethylation efficiency and qualitative applicability. Typically, however, either laborious preparation steps or cumbersome and uneconomical spin columns have been needed to obtain decent permethylation yields on small glycan samples. Here we describe a spin column-free (SCF) glycan permethylation procedure that is applicable to both O- and N-linked glycans and can be employed upstream to intact glycan analysis by MALDI-MS, ESI-MS, or glycan linkage analysis by GC-MS. The SCF procedure involves neutralization of NaOH beads by acidified phosphate buffer, which eliminates the risk of glycan oxidative degradation and avoids the use of spin columns. Optimization of the new permethylation procedure provided high permethylation efficiency for both hexose (>98%) and HexNAc (>99%) residues-yields which were comparable to (or better than) those of some widely-used spin column-based procedures. A light vs. heavy labelling approach was employed to compare intact glycan yields from a popular spin-column based approach to the SCF approach. Recovery of intact N-glycans was significantly better with the SCF procedure (p < 0.05), but overall yield of O-glycans was similar or slightly diminished (p < 0.05 for tetrasaccharides or smaller). When the SCF procedure was employed upstream to hydrolysis, reduction and acetylation for glycan linkage analysis of pooled glycans from unfractionated blood plasma, analytical reproducibility was on par with that from previous spin column-based "glycan node" analysis results. When applied to blood plasma samples from stage III-IV breast cancer patients (n = 20) and age-matched controls (n = 20), the SCF procedure facilitated identification of three glycan nodes with significantly different distributions between the cases and controls (ROC c-statistics > 0.75; p < 0.01). In summary, the SCF permethylation procedure expedites and economizes both intact glycan analysis and linkage analysis of glycans from whole biospecimens.

A spin column-free approach to sodium hydroxide-based glycan permethylation†

PubMed Central

Hu, Yueming; Borges, Chad R.

2018-01-01

Glycan permethylation was introduced as a tool to facilitate the study of glycans in 1903. Since that time, permethylation procedures have been continually modified to improve permethylation efficiency and qualitative applicability. Typically, however, either laborious preparation steps or cumbersome and uneconomical spin columns have been needed to obtain decent permethylation yields on small glycan samples. Here we describe a spin column-free (SCF) glycan permethylation procedure that is applicable to both O- and N-linked glycans and can be employed upstream to intact glycan analysis by MALDI-MS, ESI-MS, or glycan linkage analysis by GC-MS. The SCF procedure involves neutralization of NaOH beads by acidified phosphate buffer, which eliminates the risk of glycan oxidative degradation and avoids the use of spin columns. Optimization of the new permethylation procedure provided high permethylation efficiency for both hexose (>98%) and HexNAc (>99%) residues—yields which were comparable to (or better than) those of some widely-used spin column-based procedures. A light vs. heavy labelling approach was employed to compare intact glycan yields from a popular spin-column based approach to the SCF approach. Recovery of intact N-glycans was significantly better with the SCF procedure (p < 0.05), but overall yield of O-glycans was similar or slightly diminished (p < 0.05 for tetrasaccharides or smaller). When the SCF procedure was employed upstream to hydrolysis, reduction and acetylation for glycan linkage analysis of pooled glycans from unfractionated blood plasma, analytical reproducibility was on par with that from previous spin column-based “glycan node” analysis results. When applied to blood plasma samples from stage III–IV breast cancer patients (n = 20) and age-matched controls (n = 20), the SCF procedure facilitated identification of three glycan nodes with significantly different distributions between the cases and controls (ROC c-statistics > 0.75; p < 0.01). In summary, the SCF permethylation procedure expedites and economizes both intact glycan analysis and linkage analysis of glycans from whole biospecimens. PMID:28635997

Plasma focus neutron anisotropy measurements and influence of a deuteron beam obstacle

NASA Astrophysics Data System (ADS)

Talebitaher, A.; Springham, S. V.; Rawat, R. S.; Lee, P.

2017-03-01

The deuterium-deuterium (DD) fusion neutron yield and anisotropy were measured on a shot-to-shot basis for the NX2 plasma focus (PF) device using two beryllium fast-neutron activation detectors at 0° and 90° to the PF axis. Measurements were performed for deuterium gas pressures in the range 6-16 mbar, and positive correlations between neutron yield and anisotropy were observed at all pressures. Subsequently, at one deuterium gas pressure (13 mbar), the contribution to the fusion yield produced by the forwardly-directed D+ ion beam, emitted from the plasma pinch, was investigated by using a circular Pyrex plate to obstruct the beam and suppress its fusion contribution. Neutron measurements were performed with the obstacle positioned at two distances from the anode tip, and also without the obstacle. It was found that 80% of the neutron yield originates in the plasma pinch column and just above that. In addition, proton pinhole imaging was performed from the 0° and 90° directions to the pinch. The obtained proton images are consistent with the conclusion that DD fusion is concentrated ( 80%) in the pinch column region.

40 CFR 799.6756 - TSCA partition coefficient (n-octanol/water), generator column method.

Code of Federal Regulations, 2013 CFR

2013-07-01

... method, or any other reliable quantitative procedure must be used for those compounds that do not absorb... any other reliable quantitative method, aqueous solutions from the generator column enter a collecting... Solubilities and Octanol-Water Partition Coefficients of Hydrophobic Substances,” Journal of Research of the...

40 CFR 799.6756 - TSCA partition coefficient (n-octanol/water), generator column method.

Code of Federal Regulations, 2014 CFR

2014-07-01

... method, or any other reliable quantitative procedure must be used for those compounds that do not absorb... any other reliable quantitative method, aqueous solutions from the generator column enter a collecting... Solubilities and Octanol-Water Partition Coefficients of Hydrophobic Substances,” Journal of Research of the...

Characterization of Microstructure and Thermal Properties of YSZ Coatings Obtained by Axial Suspension Plasma Spraying (ASPS)

NASA Astrophysics Data System (ADS)

Ganvir, Ashish; Curry, Nicholas; Björklund, Stefan; Markocsan, Nicolaie; Nylén, Per

2015-10-01

The paper aims at demonstrating various microstructures which can be obtained using the suspension spraying technique and their respective significance in enhancing the thermal insulation property of a thermal barrier coating. Three different types of coating microstructures are discussed which were produced by the Axial Suspension Plasma Spraying. Detailed characterization of coatings was then performed. Optical and scanning electron microscopy were utilized for microstructure evaluations; x-ray diffraction for phase analysis; water impregnation, image analysis, and mercury intrusion porosimetry for porosity analysis, and laser flash analysis for thermal diffusivity measurements were used. The results showed that Axial Suspension Plasma Spraying can generate vertically cracked, porous, and feathery columnar-type microstructures. Pore size distribution was found in micron, submicron, and nanometer range. Higher overall porosity, the lower density of vertical cracks or inter-column spacing, and higher inter-pass porosity favored thermal insulation property of the coating. Significant increase in thermal diffusivity and conductivity was found at higher temperature, which is believed to be due to the pore rearrangement (sintering and pore coarsening). Thermal conductivity values for these coatings were also compared with electron beam physical vapor deposition (EBPVD) thermal barrier coatings from the literature and found to be much lower.

Interpenetration and deflection phenomena in collisions between supersonic, magnetized, tungsten plasma flows diagnosed using high resolution optical Thomson scattering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swadling, G. F.; Lebedev, S. V.; Burdiak, G.

An optical Thomson scattering diagnostic has been used to investigate collisions between supersonic, magnetized plasma flows, in particular the transition from collisionless to collisional interaction dynamics. These flows were produced using tungsten wire array z-pinches, driven by the 1.4 MA 240 ns Magpie generator at Imperial College London. Measurements of the collective-mode Thomson scattering ion-feature clearly indicate that the ablation flows are interpenetrating at 100 ns (after current start), and this interpenetration continues until at least 140 ns. The Thomson spectrum at 150 ns shows a clear change in the dynamics of the stream interactions, transitioning towards a collisional, shock-like interaction of the streamsmore » near the axis. The Thomson scattering data also provide indirect evidence of the presence of a significant toroidal magnetic field embedded in the “precursor” plasma near the axis of the array over the period 100–140 ns; these observations are in agreement with previous measurements [Swadling et al., Phys. Rev. Lett. 113, 035003 (2014)]. The Thomson scattering measurements at 150 ns suggest that this magnetic field must collapse at around the time the dense precursor column begins to form.« less

Investigation into Generation of Micro Features by Localised Electrochemical Deposition

NASA Astrophysics Data System (ADS)

Debnath, Subhrajit; Laskar, Hanimur Rahaman; Bhattacharyya, B.

2017-11-01

With the fast advancement of technology, localised electrochemical deposition (LECD) is becoming very advantageous in generating high aspect ratio micro features to meet the steep demand in modern precision industries of the present world. Except many other advantages, this technology is highly uncomplicated and economical for fabricating metal micro-parts with in micron ranges. In the present study, copper micro-columns have been fabricated utilizing LECD process. Different process parameters such as voltage, frequency, duty ratio and electrolyte concentration, which affect the deposition performance have been identified and their effects on deposition performances such as deposition rate, height and diameter of the micro-columns have been experimentally investigated. Taguchi's methodology has been used to study the effects as well as to obtain the optimum values of process parameters so that localised deposition with best performance can be achieved. Moreover, the generated micro-columns were carefully observed under optical and scanning electron microscope from where the surface quality of the deposited micro-columns has been studied qualitatively. Also, an array of copper micro-columns has been fabricated on stainless steel (SS-304) substrate for further exploration of LECD process capability.

Laboratory Studies on the Charging of Dust Grains in a Plasma

NASA Astrophysics Data System (ADS)

Xu, Wenjun

1993-01-01

The charging of dust grains by the surrounding plasma is studied in a dusty plasma device (DPD) (Xu, W., B. Song, R. L. Merlino, and N. D'Angelo, Rev. Sci. Instrum., 63, 5266, 1992). The dusty plasma device consists of a rotating-drum dust dispersal device used in conjunction with an existing Q-machine, to produce extended, steady state, magnetized plasma columns. The dust density in the dust chamber is controlled by the drum rotation speed. The device is capable of generating a dusty plasma in which as much as about 90% of the negative charge is attached to the dust grains of 1-10mu m size. Measurements of the dust parameter eta, the percentage of negative charge on free electrons in the dusty plasma, are presented. The dust parameter eta is found to depend on the rotational speed of the dust chamber, plasma density and the type and size of different dust. The dust parameter eta is calculated from a pair of Langmuir curves taken with and without dust under the same conditions. The operation of the dust chamber as described above has been confirmed by the agreement between the measurements of eta and the direct mechanical measurements consisting of weighing dust samples collected within the rotation dust chamber, at different rotation rates. By varying the ratio d/lambda_ {rm D} between the intergrain distance and the plasma Debye length, the effects predicted by Goertz and Ip (Goertz, C. K., and W-H. Ip, Geophys. Res. Lett., 11, 349, 1984), and subsequently reanalyzed in a more general fashion by Whipple et al. (Whipple, E. C., T. G. Northrop, and D. A. Mendis, J. Geophys. Rev., 90, 7405, 1985), as "isolated" dust grains become "closely packed" grains, have been demonstrated experimentally (Xu, W., N. D'Angelo, and R. L. Merlino, J. Geophys. Rev., 98, 7843, 1993). Similar results are presented and compared for two types of dust, kaolin and Al_2O _3, which have been studied in the experiment.

Casingless down-hole for sealing an ablation volume and obtaining a sample for analysis

DOEpatents

Noble, D.T.; Braymen, S.D.; Anderson, M.S.

1996-10-01

A casing-less down hole sampling system for acquiring a subsurface sample for analysis using an inductively coupled plasma system is disclosed. The system includes a probe which is pushed into the formation to be analyzed using a hydraulic ram system. The probe includes a detachable tip member which has a soil point and a barb, with the soil point aiding the penetration of the earth, and the barb causing the tip member to disengage from the probe and remain in the formation when the probe is pulled up. The probe is forced into the formation to be tested, and then pulled up slightly, to disengage the tip member and expose a column of the subsurface formation to be tested. An instrumentation tube mounted in the probe is then extended outward from the probe to longitudinally extend into the exposed column. A balloon seal mounted on the end of the instrumentation tube allows the bottom of the column to be sealed. A source of laser radiation is emitted from the instrumentation tube to ablate a sample from the exposed column. The instrumentation tube can be rotated in the probe to sweep the laser source across the surface of the exposed column. An aerosol transport system carries the ablated sample from the probe to the surface for testing in an inductively coupled plasma system. By testing at various levels in the down-hole as the probe is extracted from the soil, a profile of the subsurface formation may be obtained. 9 figs.

Simultaneous determination of D-aspartic acid and D-glutamic acid in rat tissues and physiological fluids using a multi-loop two-dimensional HPLC procedure.

PubMed

Han, Hai; Miyoshi, Yurika; Ueno, Kyoko; Okamura, Chieko; Tojo, Yosuke; Mita, Masashi; Lindner, Wolfgang; Zaitsu, Kiyoshi; Hamase, Kenji

2011-11-01

For a metabolomics study focusing on the analysis of aspartic and glutamic acid enantiomers, a fully automated two-dimensional HPLC system employing a microbore-ODS column and a narrowbore-enantioselective column was developed. By using this system, a detailed distribution of D-Asp and D-Glu besides L-Asp and L-Glu in mammals was elucidated. For the total analysis concept, the amino acids were first pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) to be sensitively and fluorometrically detected. For the non-stereoselective separation of the analytes in the first dimension a monolithic ODS column (750 mm × 0.53 mm i.d.) was adopted, and a self-packed narrowbore-Pirkle type enantioselective column (Sumichiral OA-2500S, 250 mm × 1.5 mm i.d.) was selected for the second dimension. In the rat plasma, RSD values for intra-day and inter-day precision were less than 6.8%, and the accuracy ranged between 96.1% and 105.8%. The values of LOQ of D-Asp and D-Glu were 5 fmol/injection (0.625 nmol/g tissue). The present method was successfully applied to the simultaneous determination of free aspartic acid and glutamic acid enantiomers in 7 brain areas, 11 peripheral tissues, plasma and urine of Wistar rats. Biologically significant D-Asp values were found in various tissue samples whereas for D-Glu the values were very low possibly indicating less significance. Copyright © 2011 Elsevier B.V. All rights reserved.

Cross flow cyclonic flotation column for coal and minerals beneficiation

DOEpatents

Lai, Ralph W.; Patton, Robert A.

2000-01-01

An apparatus and process for the separation of coal from pyritic impurities using a modified froth flotation system. The froth flotation column incorporates a helical track about the inner wall of the column in a region intermediate between the top and base of the column. A standard impeller located about the central axis of the column is used to generate a centrifugal force thereby increasing the separation efficiency of coal from the pyritic particles and hydrophillic tailings.

Cyclotron harmonic lines in the thermal magnetic fluctuation spectrum of spiraling electrons in plasmas

NASA Astrophysics Data System (ADS)

Stenzel, R. L.; Golubyatnikov, G.

1993-10-01

Radio frequency (rf) magnetic fluctuations B˜ have been measured with loop antennas in a large pulsed discharge plasma column (ne≲1012 cm-3, kTe≲3 eV, B0≂20 G, Ar, 2×10-4 Torr, 1 m diam×2.5 m length). A 1/f-like noise spectrum is observed in the whistler wave regime (ωce1/2ωci1/2<ω<ωce) both in the Maxwellian afterglow plasma and in the active discharge which contains energetic (45 eV) electrons. Discrete emission lines at the electron cyclotron frequency and its harmonics are found only in the presence of spiraling energetic electrons. These are naturally present in the active discharge but have also been injected as a controlled oblique electron beam into the Maxwellian afterglow plasma. In the latter case up to 15 cyclotron harmonic lines with weak amplitude decay B˜z(ω) are generated in the beam flux tube. From two-point correlation measurements it is shown that the line spectrum is due to ballistic beam modes rather than plasma eigenmodes driven unstable by the beam. The lines evolve from broadband thermal current fluctuations of the beam through a filtering effect. Those fluctuations which rotate synchronously with the ordered cyclotron motion (ω=nωc) constructively interfere (k∥=0) and produce coherent solenoidal rf fields, while others interfere destructively. Axial and azimuthal phase velocity measurements for rf-modulated beams clearly demonstrate the filtering effect. In the present parameter regime (ωp≫ωc) the fluctuations are evanescent and localized near the electron flux tube (rc≳c/ωp). In low density plasmas the fluctuations may couple to propagating electromagnetic waves and be observable externally as in earlier observations by Landauer or Ikegami.

Cylindrical neutron generator

DOEpatents

Leung, Ka-Ngo [Hercules, CA

2008-04-22

A cylindrical neutron generator is formed with a coaxial RF-driven plasma ion source and target. A deuterium (or deuterium and tritium) plasma is produced by RF excitation in a cylindrical plasma ion generator using an RF antenna. A cylindrical neutron generating target is coaxial with the ion generator, separated by plasma and extraction electrodes which contain many slots. The plasma generator emanates ions radially over 360.degree. and the cylindrical target is thus irradiated by ions over its entire circumference. The plasma generator and target may be as long as desired. The plasma generator may be in the center and the neutron target on the outside, or the plasma generator may be on the outside and the target on the inside. In a nested configuration, several concentric targets and plasma generating regions are nested to increase the neutron flux.

Cylindrical neutron generator

DOEpatents

Leung, Ka-Ngo

2005-06-14

A cylindrical neutron generator is formed with a coaxial RF-driven plasma ion source and target. A deuterium (or deuterium and tritium) plasma is produced by RF excitation in a cylindrical plasma ion generator using an RF antenna. A cylindrical neutron generating target is coaxial with the ion generator, separated by plasma and extraction electrodes which contain many slots. The plasma generator emanates ions radially over 360.degree. and the cylindrical target is thus irradiated by ions over its entire circumference. The plasma generator and target may be as long as desired. The plasma generator may be in the center and the neutron target on the outside, or the plasma generator may be on the outside and the target on the inside. In a nested configuration, several concentric targets and plasma generating regions are nested to increase the neutron flux.

Cylindrical neutron generator

DOEpatents

Leung, Ka-Ngo [Hercules, CA

2009-12-29

A cylindrical neutron generator is formed with a coaxial RF-driven plasma ion source and target. A deuterium (or deuterium and tritium) plasma is produced by RF excitation in a cylindrical plasma ion generator using an RF antenna. A cylindrical neutron generating target is coaxial with the ion generator, separated by plasma and extraction electrodes which contain many slots. The plasma generator emanates ions radially over 360.degree. and the cylindrical target is thus irradiated by ions over its entire circumference. The plasma generator and target may be as long as desired. The plasma generator may be in the center and the neutron target on the outside, or the plasma generator may be on the outside and the target on the inside. In a nested configuration, several concentric targets and plasma generating regions are nested to increase the neutron flux.

Electron-ion hybrid instability experiment upgrades to the Auburn Linear Experiment for Instability Studies.

PubMed

DuBois, A M; Arnold, I; Thomas, E; Tejero, E; Amatucci, W E

2013-04-01

The Auburn Linear EXperiment for Instability Studies (ALEXIS) is a laboratory plasma physics experiment used to study spatially inhomogeneous flows in a magnetized cylindrical plasma column that are driven by crossed electric (E) and magnetic (B) fields. ALEXIS was recently upgraded to include a small, secondary plasma source for a new dual source, interpenetrating plasma experiment. Using two plasma sources allows for highly localized electric fields to be made at the boundary of the two plasmas, inducing strong E × B velocity shear in the plasma, which can give rise to a regime of instabilities that have not previously been studied in ALEXIS. The dual plasma configuration makes it possible to have independent control over the velocity shear and the density gradient. This paper discusses the recent addition of the secondary plasma source to ALEXIS, as well as the plasma diagnostics used to measure electric fields and electron densities.

Micro-column plasma emission liquid chromatograph. [Patent application

DOEpatents

Gay, D.D.

1982-08-12

In a direct current plasma emission spectrometer for use in combination with a microcolumn liquid chromatograph, an improved plasma source unit is claimed. The plasma source unit includes a quartz capillary tube having an inlet means, outlet off gas means and a pair of spaced electrodes defining a plasma region in the tube. The inlet means is connected to and adapted to receive eluant of the liquid chromatograph along with a stream of plasma-forming gas. There is an opening through the wall of the capillary tube penetrating into the plasma region. A soft glass capillary light pipe is disposed at the opening, is connected to the spectrometer, and is adapted to transmit light passing from the plasma region to the spectrometer. There is also a source of electromotive force connected to the electrodes sufficient to initiate and sustain a plasma in the plasma region of the tube.

Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer.

PubMed

Hong, Chang-Sook; Funk, Sonja; Muller, Laurent; Boyiadzis, Michael; Whiteside, Theresa L

2016-01-01

Isolation from human plasma of exosomes that retain functional and morphological integrity for probing their protein, lipid and nucleic acid content is a priority for the future use of exosomes as biomarkers. A method that meets these criteria and can be scaled up for patient monitoring is thus desirable. Plasma specimens (1 mL) of patients with acute myeloid leukaemia (AML) or a head and neck squamous cell carcinoma (HNSCC) were differentially centrifuged, ultrafiltered and fractionated by size exclusion chromatography in small disposable columns (mini-SEC). Exosomes were eluted in phosphate-buffered saline and were evaluated by qNano for particle size and counts, morphology by transmission electron microscopy, protein content, molecular profiles by western blots, and for ability to modify functions of immune cells. Exosomes eluting in fractions #3-5 had a diameter ranging from 50 to 200 nm by qNano, with the fraction #4 containing the bulk of clean, unaggregated exosomes. The exosome elution profiles remained constant for repeated runs of the same plasma. Larger plasma volumes could be fractionated running multiple mini-SEC columns in parallel. Particle concentrations per millilitre of plasma in #4 fractions of AML and HNSCC were comparable and were higher (p<0.003) than those in normal controls. Isolated AML exosomes co-incubated with normal human NK cells inhibited NKG2D expression levels (p<0.004), and HNSCC exosomes suppressed activation (p<0.01) and proliferation of activated T lymphocytes (p<0.03). Mini-SEC allows for simple and reproducible isolation from human plasma of exosomes retaining structural integrity and functional activity. It enables molecular/functional analysis of the exosome content in serial specimens of human plasma for clinical applications.

Plasma rich in growth factors (PRGF-Endoret) stimulates proliferation and migration of primary keratocytes and conjunctival fibroblasts and inhibits and reverts TGF-beta1-Induced myodifferentiation.

PubMed

Anitua, Eduardo; Sanchez, Mikel; Merayo-Lloves, Jesus; De la Fuente, Maria; Muruzabal, Francisco; Orive, Gorka

2011-08-01

Plasma rich in growth factors (PRGF-Endoret) technology is an autologous platelet-enriched plasma obtained from patient's own blood, which after activation with calcium chloride allows the release of a pool of biologically active proteins that influence and promote a range of biological processes including cell recruitment, and growth and differentiation. Because ocular surface wound healing is mediated by different growth factors, we decided to explore the potential of PRGF-Endoret technology in stimulating the biological processes related with fibroblast-induced tissue repair. Furthermore, the anti-fibrotic properties of this technology were also studied. Blood from healthy donors was collected, centrifuged and, whole plasma column (WP) and the plasma fraction with the highest platelet concentration (F3) were drawn off, avoiding the buffy coat. Primary human cells including keratocytes and conjunctival fibroblasts were used to perform the "in vitro" investigations. The potential of PRGF-Endoret in promoting wound healing was evaluated by means of a proliferation and migration assays. Fibroblast cells were induced to myofibroblast differentiation after the treatment with 2.5 ng/mL of TGF-β1. The capability of WP and F3 to prevent and inhibit TGF-β1-induced differentiation was evaluated. Results show that this autologous approach significantly enhances proliferation and migration of both keratocytes and conjunctival fibroblasts. In addition, plasma rich in growth factors prevents and inhibits TGF-β1-induced myofibroblast differentiation. No differences were found between WP and F3 plasma fractions. These results suggest that PRGF-Endoret could reduce scarring while stimulating wound healing in ocular surface. F3 or whole plasma column show similar biological effects in keratocytes and conjunctival fibroblast cells.

Radial scanning diagnostics of bremsstrahlung and line emission in T-10 plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nemets, A. R., E-mail: Nemets-AR@nrcki.ru; Krupin, V. A.; Klyuchnikov, L. A., E-mail: lklyuchnikov@list.ru

2016-12-15

The paper describes the scanning spectroscopic diagnostics designed for measurement of line integrated plasma radiation in two visible spectral ranges. This diagnostic system is aimed at measuring the bremsstrahlung absolute values and profile with high spatial resolution. The bremsstrahlung absolute values are used to determine the value and radial distribution of effective plasma ion charge Z{sub eff}(r) in T-10 discharges. The importance of Z{sub eff} measurement is due to its strong influence on plasma heating, confinement, and stability. The spatial distribution of emission for one of the chosen spectral lines is measured simultaneously with bremsstrahlung. The spatial resolution of measurementsmore » is ~1 cm, and the temporal resolution is up to 10 ms. The spectral equipment and methods for its calibration are described. Examples of line integrated brightness distribution in a “continuum window” of 5236 ± 6 Å and brightness of the lines C{sup 5+} (5291 Å), He{sup 1+} (4686 Å), and D{sub β} (4861 Å) are given. Flattening of the bremsstrahlung brightness profile in the central region of the plasma column in some discharges with sawtooth oscillations in the T-10 is observed. The measured effective ion charge profiles in ohmic discharges with high plasma density and low discharge currents demonstrate accumulation of light impurities at the column axis; as a consequence, quenching of sawtooth oscillations in some discharges is observed. The developed diagnostics provides necessary data for investigation of heat, particle, and current transport in the plasma of the T-10. Successful application of the obtained data on Z{sub eff}(r) for investigation of geodesic acoustic modes of plasma oscillations in the T-10 should be specially noted.« less

1986 Annual Conference on Nuclear and Space Radiation Effects, 23rd, Providence, RI, July 21-23, 1986, Proceedings

NASA Technical Reports Server (NTRS)

Ellis, Thomas D. (Editor)

1986-01-01

The present conference on the effects of nuclear and space radiation on electronic hardware gives attention to topics in the basic mechanisms of radiation effects, dosimetry and energy-dependent effects, electronic device radiation hardness assurance, SOI/SOS radiation effects, spacecraft charging and space radiation, IC radiation effects and hardening, single-event upset (SEU) phenomena and hardening, and EMP/SGEMP/IEMP phenomena. Specific treatments encompass the generation of interface states by ionizing radiation in very thin MOS oxides, the microdosimetry of meson energy deposited on 1-micron sites in Si, total dose radiation and engineering studies, plasma interactions with biased concentrator solar cells, the transient imprint memory effect in MOS memories, mechanisms leading to SEU, and the vaporization and breakdown of thin columns of water.

High-performance liquid chromatographic determination of 4,4'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone in plasma.

PubMed

Rodgers, A H; Subramanian, S; Morgan, L R

1995-08-18

An analytical method has been developed for the determination of 4,4'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone (I, trade name A-007) in plasma. Plasma samples are primed with the internal standard, 2,2'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone (II), deproteinized with acetonitrile, centrifuged and filtered prior to assay. The components are then separated on a reversed-phase column with retention times of 4.4 and 6.0 min for I and II, respectively. Ultraviolet detection at 365 nm was employed and little interference with the analyte or the internal standard was noted from other plasma components. This method has been applied to the plasma of rats and monkeys doses for pharmacokinetic and toxicity studies.

Determination of ambroxol in human plasma using LC-MS/MS.

PubMed

Kim, Hohyun; Yoo, Jeong-Yeon; Han, Sang Beom; Lee, Hee Joo; Lee, Kyung Ryul

2003-06-01

A sensitive and selective liquid chromatographic method coupled with tandem mass spectrometry (LC-MS/MS) was developed for the quantification of ambroxol in human plasma. Domperidone was used as internal standard, with plasma samples extracted using diethyl ether under basic condition. A centrifuged upper layer was then evaporated and reconstituted with 200 microl methanol. The reconstituted samples were injected into a C(18) XTerra MS column (2.1 x 30 mm) with 3.5 microm particle size. The analytical column lasted for at least 600 injections. The mobile phase was composed of 20 mM ammonium acetate in 90% acetonitrile (pH 8.8), with flow rate at 250 microl/min. The mass spectrometer was operated in positive ion mode using turbo electrospray ionization. Nitrogen was used as the nebulizer, curtain, collision, and auxiliary gases. Using MS/MS with multiple reaction monitoring (MRM) mode, ambroxol was detected without severe interferences from plasma matrix. Ambroxol produced a protonated precursor ion ([M+H](+)) at m/z 379 and a corresponding product ion at m/z 264. And internal standard (domperidone) produced a protonated precursor ion ([M+H](+)) at m/z 426 and a corresponding product ion at m/z 174. Detection of ambroxol in human plasma was accurate and precise, with quantification limit at 0.2 ng/ml. This method has been successfully applied to a study of ambroxol in human specimens.

Sensitive method for the quantitation of droloxifene in plasma and serum by high-performance liquid chromatography employing fluorimetric detection.

PubMed

Tess, D A; Cole, R O; Toler, S M

1995-12-15

A simple and highly sensitive reversed-phase fluorimetric HPLC method for the quantitation of droloxifene from rat, monkey, and human plasma as well as human serum is described. This assay employs solid-phase extraction and has a dynamic range of 25 to 10,000 pg/ml. Sample extraction (efficiencies > 86%) was accomplished using a benzenesulfonic acid (SCX) column with water and methanol rinses. Droloxifene and internal standard were eluted with 1 ml of 3.5% (v/v) ammonium hydroxide (30%) in methanol. Samples were quantitated using post-column UV-photochemical cyclization coupled with fluorimetric detection with excitation and emission wavelengths of 260 nm and 375 nm, respectively. Relative ease of sample extraction and short run times allow for the analysis of approximately 100 samples per day.

Coupling nanoliter high-performance liquid chromatography to inductively coupled plasma mass spectrometry for arsenic speciation.

PubMed

Cheng, Heyong; Shen, Lihuan; Liu, Jinhua; Xu, Zigang; Wang, Yuanchao

2018-04-01

Nanoliter high-performance liquid chromatography shows low consumption of solvents and samples, offering one of the best choices for arsenic speciation in precious samples in combination with inuctively coupled plasma mass spectrometry. A systematic investigation on coupling nanoliter high-performance liquid chromatography to inductively coupled plasma mass spectrometry from instrument design to injected sample volume and mobile phase was performed in this study. Nanoflow mobile phase was delivered by flow splitting using a conventional high-pressure pump with reuse of mobile phase waste. Dead volume was minimized to 60 nL for the sheathless interface based on the previously developed nanonebulizer. Capillary columns for nanoliter high-performance liquid chromatography were found to be sensitive to sample loading volume. An apparent difference was also found between the mobile phases for nanoliter and conventional high-performance liquid chromatography. Baseline separation of arsenite, arsenate, monomethylarsenic, and dimethylarsenic was achieved within 11 min on a 15 cm C 18 capillary column and within 12 min on a 25 cm strong anion exchange column. Detection limits of 0.9-1.8 μg/L were obtained with precisions variable in the range of 1.6-4.2%. A good agreement between determined and certified values of a certified reference material of human urine (GBW 09115) validated its accuracy along with good recoveries (87-102%). © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Applying a physical continuum model to describe the broadband X-ray spectra of accreting pulsars at high luminosity

NASA Astrophysics Data System (ADS)

Pottschmidt, Katja; Hemphill, Paul B.; Wolff, Michael T.; Cheatham, Diana M.; Iwakiri, Wataru; Gottlieb, Amy M.; Falkner, Sebastian; Ballhausen, Ralf; Fuerst, Felix; Kuehnel, Matthias; Ferrigno, Carlo; Becker, Peter A.; Wood, Kent S.; Wilms, Joern

2018-01-01

A new window for better understanding the accretion onto strongly magnetized neutron stars in X-ray binaries is opening. In these systems the accreted material follows the magnetic field lines as it approaches the neutron star, forming accretion columns above the magnetic poles. The plasma falls toward the neutron star surface at near-relativistic speeds, losing energy by emitting X-rays. The X-ray spectral continua are commonly described using phenomenological models, i.e., power laws with different types of curved cut-offs at higher energies. Here we consider high luminosity pulsars. In these systems the mass transfer rate is high enough that the accreting plasma is thought to be decelerated in a radiation-dominated radiative shock in the accretion columns. While the theory of the emission from such shocks had already been developed by 2007, a model for direct comparison with X-ray continuum spectra in xspec or isis has only recently become available. Characteristic parameters of this model are the accretion column radius and the plasma temperature, among others. Here we analyze the broadband X-ray spectra of the accreting pulsars Centaurus X-3 and 4U 1626-67 obtained with NuSTAR. We present results from traditional empirical modeling as well as successfully apply the radiation-dominated radiative shock model. We also take the opportunity to compare to similar recent analyses of both sources using these and other observations.

Estimation of Electron Temperature on Glass Spherical Tokamak (GLAST)

NASA Astrophysics Data System (ADS)

Hussain, S.; Sadiq, M.; Shah, S. I. W.; GLAST Team

2015-03-01

Glass Spherical Tokamak (GLAST) is a small spherical tokamak indigenously developed in Pakistan with an insulating vacuum vessel. A commercially available 2.45 GHz magnetron is used as pre-ionization source for plasma current startup. Different diagnostic systems like Rogowski coils, magnetic probes, flux loops, Langmuir probe, fast imaging and emission spectroscopy are installed on the device. The plasma temperature inside of GLAST, at the time of maxima of plasma current, is estimated by taking into account the Spitzer resistivity calculations with some experimentally determined plasma parameters. The plasma resistance is calculated by using Ohm's law with plasma current and loop voltage as experimentally determined inputs. The plasma resistivity is then determined by using length and area of the plasma column. Finally, the average plasma electron temperature is predicted to be 12.65eV for taking neon (Ne) as a working gas.

Thermonuclear inverse magnetic pumping power cycle for stellarator reactor

DOEpatents

Ho, Darwin D.; Kulsrud, Russell M.

1991-01-01

The plasma column in a stellarator is compressed and expanded alternatively in minor radius. First a plasma in thermal balance is compressed adiabatically. The volume of the compressed plasma is maintained until the plasma reaches a new thermal equilibrium. The plasma is then expanded to its original volume. As a result of the way a stellarator works, the plasma pressure during compression is less than the corresponding pressure during expansion. Therefore, negative work is done on the plasma over a complete cycle. This work manifests itself as a back-voltage in the toroidal field coils. Direct electrical energy is obtained from this voltage. Alternatively, after the compression step, the plasma can be expanded at constant pressure. The cycle can be made self-sustaining by operating a system of two stellarator reactors in tandem. Part of the energy derived from the expansion phase of a first stellarator reactor is used to compress the plasma in a second stellarator reactor.

Investigation of an alternating current plasma as an element selective atomic emission detector for high-resolution capillary gas chromatography and as a source for atomic absorption and atomic emission spectrometry

NASA Astrophysics Data System (ADS)

Ombaba, Jackson M.

This thesis deals with the construction and evaluation of an alternating current plasma (ACP) as an element-selective detector for high resolution capillary gas chromatography (GC) and as an excitation source for atomic absorption spectrometry (AAS) and atomic emission spectrometry (AES). The plasma, constrained in a quartz discharge tube at atmospheric pressure, is generated between two copper electrodes and utilizes helium as the plasma supporting gas. The alternating current plasma power source consists of a step-up transformer with a secondary output voltage of 14,000 V at a current of 23 mA. The device exhibits a stable signal because the plasma is self-seeding and reignites itself every half cycle. A tesla coil is not required to commence generation of the plasma if the ac voltage applied is greater than the breakdown voltage of the plasma-supporting gas. The chromatographic applications studied included the following: (1) the separation and selective detection of the organotin species, tributyltin chloride (TBT) and tetrabutyltin (TEBT), in environmental matrices including mussels (Mvutilus edullus) and sediment from Boston Harbor, industrial waste water and industrial sludge, and (2) the detection of methylcyclopentadienyl manganesetricarbonyl (MMT) and similar compounds used as gasoline additives. An ultrasonic nebulizer (common room humidifier) was utilized as a sample introduction device for aqueous solutions when the ACP was employed as an atomization source for atomic absorption spectrometry and as an excitation source for atomic emission spectrometry. Plasma diagnostic parameters studied include spatial electron number density across the discharge tube, electronic, excitation and ionization temperatures. Interference studies both in absorption and emission modes were also considered. Figures of merits of selected elements both in absorption and emission modes are reported. The evaluation of a computer-aided optimization program, Drylab GC, using spearmint oil and Environmental Protection Agency (EPA) standard mixture as probes is also discussed. The program supplied by LC Resources (Lafayette, CA) is used for separation optimization and prediction of gas chromatographic parameters. Column dead-time and average plate number were used as input data in conjunction with the retention times and peak areas of solutes at two different temperature programming rates. Once input data are entered into an IBM or IBM compatible personal computer, the program produces a 'relative resolution map' (RRM) which guides the analyst in selecting the most favorable temperature programming rate for the separation.

40 CFR 799.6756 - TSCA partition coefficient (n-octanol/water), generator column method.

Code of Federal Regulations, 2012 CFR

2012-07-01

... method, or any other reliable quantitative procedure must be used for those compounds that do not absorb... any other reliable quantitative method, aqueous solutions from the generator column enter a collecting... Research of the National Bureau of Standards, 86:361-366 (1981). (7) Fujita, T. et al. “A New Substituent...

40 CFR 799.6756 - TSCA partition coefficient (n-octanol/water), generator column method.

Code of Federal Regulations, 2010 CFR

2010-07-01

... method, or any other reliable quantitative procedure must be used for those compounds that do not absorb... any other reliable quantitative method, aqueous solutions from the generator column enter a collecting... Research of the National Bureau of Standards, 86:361-366 (1981). (7) Fujita, T. et al. “A New Substituent...

40 CFR 799.6756 - TSCA partition coefficient (n-octanol/water), generator column method.

Code of Federal Regulations, 2011 CFR

2011-07-01

... method, or any other reliable quantitative procedure must be used for those compounds that do not absorb... any other reliable quantitative method, aqueous solutions from the generator column enter a collecting... Research of the National Bureau of Standards, 86:361-366 (1981). (7) Fujita, T. et al. “A New Substituent...

Validation of a LC-fluorescence method for determination of free captopril in human plasma, using a pre-column derivatization reaction with monobromobimane.

PubMed

Tache, Florentin; Farca, Alexandru; Medvedovici, Andrei; David, Victor

2002-05-15

Both derivatization of free captopril in human plasma samples using monobromobimane as fluorescent label and the corresponding HPLC-fluorescence detection (FLD) method were validated. Calibration curve for the fluorescent captopril derivative in plasma samples is linear in the ppb-ppm range with a detection limit of 4 ppb and an identification limit of 10 ppb (P%: 90; nu > or = 5). These methods were successfully applied on bioequivalence studies carried out on some marketed pharmaceutical formulations.

X-ray line emission from the Puppis A supernova remnant - Oxygen lines

NASA Technical Reports Server (NTRS)

Winkler, P. F.; Clark, G. W.; Markert, T. H.; Petre, R.; Canizares, C. R.

1981-01-01

Six prominent X-ray emission lines of O VII and O VIII have been detected from a portion of the Puppis A supernova remnant in observations with the Einstein Observatory Focal Plane Crystal Spectrometer. The lines are sufficiently well resolved to serve as diagnostics of the emitting plasma. From the relative intensities of the lines, it is inferred that the population of O VIII is about 1.5 times that of O VII, and that electron collisions are the dominant excitation mechanism in the plasma. A locus of allowed electron temperatures and interstellar-absorption column densities is derived: 1.5 x 10 to the 6th K, and (2-6) x 10 to the 21st per sq cm. The data are consistent with either a thin plasma source in equilibrium at a temperature of 2.2 x 10 to the 6th K with a column density of 4 x 10 to the 21st per sq cm, or with a nonequilibrium source in which the electrons have been shock-heated to a higher temperature and oxygen is underionized.

Precolumn derivatization followed by liquid chromatographic separation and determination of tramiprosate in rat plasma by fluorescence detector: application to pharmacokinetics.

PubMed

Rao, R Nageswara; Maurya, Pawan K; Shinde, Dhananjay D; Khalid, Sara

2011-05-15

Alzheimer disease (AD) is characterized pathologically by extracellular amyloid deposits composed of amyloid β (Aβ) protein. A simple and rapid method using HPLC with fluorescence detector was developed and validated for determination of tramiprosate in rat plasma. Pre-column derivatization of the deproteinized rat plasma was carried out using o-phthaldialdehyde (OPA) as a fluorescent reagent in presence of 3-mercaptopropionic acid. The liquid chromatographic separation was achieved on a Kromasil C18 column using methanol:acetonitrile: 20 mM phosphate buffer pH 7.5 (8.0:17.5:74.5 v/v/v) as a mobile phase in an isocratic elution mode. The eluents were monitored by a fluorescence detector set at 330 and 450 nm of excitation and emission wavelength respectively. Vigabatrin was used as an internal standard. The method was linear within the range 30.0-1000.0 ng/mL. Design of experiments (DOE) was used to evaluate the robustness of the method. The developed method was applied to study the pharmacokinetics of tramiprosate in rats. Copyright © 2011. Published by Elsevier B.V.

Determination of penicillamine in pharmaceuticals and human plasma by capillary electrophoresis with in-column fiber optics light-emitting diode induced fluorescence detection.

PubMed

Yang, Xiupei; Yuan, Hongyan; Wang, Chunling; Su, Xiaodong; Hu, Li; Xiao, Dan

2007-10-18

In this paper, a capillary electrophoresis (CE) system with in-column fiber optics light-emitting diode (LED) induced fluorescence detection was developed for the determination of penicillamine (PA). The influence of buffer concentration, buffer pH, applied voltage and injection time was systematically investigated. Optimum separation conditions were obtained with 10 mM borate buffer at pH 9.1, applied voltage 20 kV and 8 s hydrodynamic injection at 30 mbar. The detection system displayed linear dynamic range from 3.2 x 10(-7) to 4.8 x 10(-5) mol L(-1) with a correlation coefficient of 0.9991 and good repeatability (R.S.D.=2.46%). The method was applied to the determination of PA in commercial tablets and human plasma, which the recoveries of standard PA added to tablets and human plasma sample were found to be in the range of 96.26-102.68 and 91.10-99.35%, respectively. The proposed method is cheap, rapid, easy, and accurate, and can be successfully applied to the formulation analysis and bioanalysis.

Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.

PubMed

Ali, Imran; Al-Othman, Zeid A; Nagae, Norikaju; Gaitonde, Vinay D; Dutta, Kamlesh K

2012-12-01

New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate sample loading capacity and low back pressure. Chemistries of these columns are C(8), C(18), RP-Amide, hydrophilic interaction liquid chromatography, penta fluorophenyl (PFP), F5, and RP-aqua. Normally, the silica gel particles are of 2.7 and 1.7 μm as total and inner solid core diameters, respectively, with 0.5-μm-thick of outer porous layer having 90 Å pore sizes and 150 m(2)/g surface area. This article describes these new generation columns with special emphasis on their textures and chemistries, separations, optimization, and comparison (inter and intra stationary phases). Besides, future perspectives have also been discussed. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A New Procedure for Determination of Electron Temperatures and Electron Concentrations by Thomson Scattering and Analytical Plasmas

DTIC Science & Technology

1988-10-28

Spectrochim. Acta 40B, 1211 (1985). 24 30. K. A. MARSHALL and G. M. HIEFTJE, Spectrochim. Acca (submitted, 1987). 31. H. J. KUNZE, Plasma Diagnostics, Ed...13 0,47689017-16 0 .4 F6 S6 7 E.16 26 Table 2. Correction table for Nd/YAG laser. Real (true) Te (top line) and Ne (left column) values and

[Use of adsorption methods for plasma component apheresis].

PubMed

Bang, B; Heegaard, N H

1991-11-25

Plasma-apheresis is a nonspecific and wasteful intervention requiring the use of potentially infectious and expensive replacement fluids. Selective removal of the unwanted plasma component circumvents most of the problems. For selective binding and removal of plasma components adsorption methods based on the principles of affinity chromatography have been useful. The ideal adsorption column still does not exist, but the number of clinical applications is increasing. The results vary, but the treatment has been used with success in hypercholesterolemia, and in patients with hemophilia with antifactor antibodies and patients with antibodies directed towards HLA-antigens awaiting renal transplantation. In conclusion selective plasma component-apheresis is an improvement in some diseases as compared to conventional plasma-apheresis. The technique is still being improved but large clinical trials examining the effects of plasma-component-apheresis have not yet been published.

High‐precision determination of lithium and magnesium isotopes utilising single column separation and multi‐collector inductively coupled plasma mass spectrometry

PubMed Central

Misra, Sambuddha; Lloyd, Nicholas; Elderfield, Henry; Bickle, Mike J.

2017-01-01

Rationale Li and Mg isotopes are increasingly used as a combined tool within the geosciences. However, established methods require separate sample purification protocols utilising several column separation procedures. This study presents a single‐step cation‐exchange method for quantitative separation of trace levels of Li and Mg from multiple sample matrices. Methods The column method utilises the macro‐porous AGMP‐50 resin and a high‐aspect ratio column, allowing quantitative separation of Li and Mg from natural waters, sediments, rocks and carbonate matrices following the same elution protocol. High‐precision isotope determination was conducted by multi‐collector inductively coupled plasma mass spectrometry (MC‐ICPMS) on the Thermo Scientific™ NEPTUNE Plus™ fitted with 1013 Ω amplifiers which allow accurate and precise measurements at ion beams ≤0.51 V. Results Sub‐nanogram Li samples (0.3–0.5 ng) were regularly separated (yielding Mg masses of 1–70 μg) using the presented column method. The total sample consumption during isotopic analysis is <0.5 ng Li and <115 ng Mg with long‐term external 2σ precisions of ±0.39‰ for δ7Li and ±0.07‰ for δ26Mg. The results for geological reference standards and seawater analysed by our method are in excellent agreement with published values despite the order of magnitude lower sample consumption. Conclusions The possibility of eluting small sample masses and the low analytical sample consumption make this method ideal for samples of limited mass or low Li concentration, such as foraminifera, mineral separates or dilute river waters. PMID:29078008

High radioactive concentration of 99mTc from a zirconium [99Mo]molybdate gel generator using an acidic alumina column for purification and concentration.

PubMed

Sarkar, S K; Saraswathy, P; Arjun, G; Ramamoorthy, N

2004-06-01

Newer applications of radiopharmaceuticals in nuclear medicine require pertechnetate of moderate to high radioactive concentration. Hence there is a need to develop simple procedures for the concentration of pertechnetate, and such a procedure is given in this paper. Ten to 20 ml of sodium [Tc]pertechnetate eluted in de-ionized water from a zirconium [Mo]molybdate (ZrMo) gel column generator was passed through 2 g of an acidic alumina bed (35 x 8 mm) in order to remove the co-eluted traces of Mo and to retain the pertechnetate. The retained pertechnetate was then re-eluted, quantitatively, in 3 ml of normal saline, from the alumina column. About a 4-fold increase in radioactive concentration of Tc was obtained (cf. approximately 10-12 ml normal saline is required for the elution of Tc from the gel column). Generators containing up to 22.2 GBq (600 mCi) Mo in 6-7 g ZrMo gel column (35 x 13 mm) were prepared and a radioactive concentration of Tc up to 4 GBq x ml (110 mCi x ml) was obtained on the first day of use. The overall recovery of Tc was >90%, Mo breakthrough was 10 to 10% and the duration of concentration was 3-5 min. The chemical impurity in terms of Al, Mo and Zr was <10 ppm each. The same procedure for the concentration of pertechnetate was applied to generators with 12-15 g ZrMo gel beds to obtain a higher capacity Tc gel generator, with similar findings.

Dogs lap using acceleration-driven open pumping

PubMed Central

Gart, Sean; Socha, John J.; Vlachos, Pavlos P.; Jung, Sunghwan

2015-01-01

Dogs lap because they have incomplete cheeks and cannot suck. When lapping, a dog’s tongue pulls a liquid column from the bath, suggesting that the hydrodynamics of column formation are critical to understanding how dogs drink. We measured lapping in 19 dogs and used the results to generate a physical model of the tongue’s interaction with the air–fluid interface. These experiments help to explain how dogs exploit the fluid dynamics of the generated column. The results demonstrate that effects of acceleration govern lapping frequency, which suggests that dogs curl the tongue to create a larger liquid column. Comparing lapping in dogs and cats reveals that, despite similar morphology, these carnivores lap in different physical regimes: an unsteady inertial regime for dogs and steady inertial regime for cats. PMID:26668382

Preparation of a poly(styrene-co-DPHA-co-EDMA) monolith and its application for the separation of small molecules and biomacromolecules by HPLC.

PubMed

Wang, Xixi; Li, Xueying; Jiang, Xiaoya; Dong, Peipei; Liu, Haiyan; Bai, Ligai; Yan, Hongyuan

2017-04-01

A high performance liquid chromatography (HPLC) monolithic column was prepared by redox polymerization of styrene, dipentaerythritol hexaacrylate (DPHA) and ethylene glycol dimethacrylate (EDMA) in a porogen system of n-propanol/PEG400. The monolith was characterized by scanning electron microscopy (SEM), mercury intrusion porosimetry (MIP) and the results indicated that the monolith had a stable and homogeneous structure. The porosity of the monolithic column was 75.86% and average pore diameter was 2.1µm. Several alkylbenzenes and anilines were used to evaluate the column performance in terms of hydrophobicity. Then the column was applied to separate small molecules including phytosterol and BSA tryptic digest. Finally, five standard proteins, egg white and plasma were separated respectively and high separation capacity of protein was obtained. Copyright © 2016 Elsevier B.V. All rights reserved.

Simultaneous analysis of thiamphenicol and its prodrug thiamphenicol glycinate in human plasma and urine by high performance liquid chromatography: application to pharmacokinetic study.

PubMed

Chen, Xijing; Yang, Bing; Ni, Liang; Wang, Guangji

2006-06-07

A simple and sensitive method for simultaneous determination of the active compound, thiamphenicol (TAP) and its prodrug, thiamphenicol glycinate (TG) in human plasma and urine is described. The procedure involved extraction of TG and TAP with ethyl acetate (plasma) or 100-fold dilution with the mobile phase (urine) followed by determination by reversed-phase high performance liquid chromatography (HPLC) with UV detection at 224 nm. Separation of the compounds was achieved on a column packed with Hypersil ODS2. The mobile phase consisted of acetonitrile-water containing 0.003 M tetrabutyl ammonium bromide and 0.056 M ammonium acetate (87:13, v/v) with a flow rate of 1.0 ml/min. The chromatograms did not contain interfering peaks due to the suitable extraction procedure and chromatographic conditions. The calibration curves of TG and TAP were linear ranging from 0.78 to 100 microg/ml in plasma and in urine. The intra-day and inter-day relative standard deviations (S.D.) were less than 10%. The recoveries of TG and TAP in plasma and urine were above 80%. TG was not stable in plasma samples and after extraction at ambient temperature or in freeze-thaw cycles, and hence the samples for injection on HPLC column should be stored in refrigerator or under ice cooling prior to analysis, and the plasma samples should not experience the freeze-thaw cycle more than one time. Unlike TAP, TG could not be detected in most urine samples. Application of this method demonstrated that it was feasible for the clinical pharmacokinetic study.

Cellular Targets of Dietary Polyphenol Resveratrol

DTIC Science & Technology

2005-03-01

attempts to generate affinity columns tagged with other polyphenols, e.g., epigallocatechin gallate ( EGCG ). Conceivably such columns, if generated, would...Similar affinity chromatography with the related polyphenol Epigallocatechin gallate does not produce similar results.” Answer: We did not make...addition, the PI does not provid expression. If there is “increased ex many bind the resveratrol affinity co related polyphenol Epigallocatechin Response

Guiding of laser pulses in plasma waveguides created by linearly-polarized femtosecond laser pulses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lemos, N.; Cardoso, L.; Geada, J.

We experimentally demonstrate that plasma waveguides produced with ultra-short laser pulses (sub-picosecond) in gas jets are capable of guiding high intensity laser pulses. This scheme has the unique ability of guiding a high-intensity laser pulse in a plasma waveguide created by the same laser system in the very simple and stable experimental setup. A hot plasma column was created by a femtosecond class laser that expands into an on-axis parabolic low density profile suitable to act as a waveguide for high intensity laser beams. We have successfully guided ~10 15 W cm -2 laser pulses in a 8 mm longmore » hydrogen plasma waveguide with a 35% guiding efficiency.« less

Guiding of laser pulses in plasma waveguides created by linearly-polarized femtosecond laser pulses

DOE PAGES

Lemos, N.; Cardoso, L.; Geada, J.; ...

2018-02-16

We experimentally demonstrate that plasma waveguides produced with ultra-short laser pulses (sub-picosecond) in gas jets are capable of guiding high intensity laser pulses. This scheme has the unique ability of guiding a high-intensity laser pulse in a plasma waveguide created by the same laser system in the very simple and stable experimental setup. A hot plasma column was created by a femtosecond class laser that expands into an on-axis parabolic low density profile suitable to act as a waveguide for high intensity laser beams. We have successfully guided ~10 15 W cm -2 laser pulses in a 8 mm longmore » hydrogen plasma waveguide with a 35% guiding efficiency.« less

Two-dimensional positive column structure with dust cloud: Experiment and nonlocal kinetic simulation

NASA Astrophysics Data System (ADS)

Zobnin, A. V.; Usachev, A. D.; Petrov, O. F.; Fortov, V. E.; Thoma, M. H.; Fink, M. A.

2018-03-01

The influence of a dust cloud on the structure of the positive column of a direct current gas discharge in a cylindrical glass tube under milligravity conditions has been studied both experimentally and numerically. The discharge was produced in neon at 60 Pa in a glass tube with a diameter of 30 mm at a discharge current 1 mA. Spherical monodisperse melamine formaldehyde dust particles with a diameter of 6.86 μm were injected into the positive column and formed there a uniform dust cloud with a maximum diameter of 14.4 mm. The shape of the cloud and the dust particle number density were measured. The cloud was stationary in the radial direction and slowly drifted in the axial direction. It was found that in the presence of the dust cloud, the intensity of the neon spectral line with a wavelength by 585.25 nm emitted by the discharge plasma increased by 2.3 times and 2 striations appeared on the anode side of the cloud. A numerical simulation of the discharge was performed using the 2D (quasi-3D) nonlocal self-consistent kinetic model of a longitudinally inhomogeneous axially symmetric positive column [Zobnin et al., Phys. Plasmas 21, 113503 (2014)], which was supplemented by a program module performing a self-consistent calculation of dust particle charges, the plasma recombination rate on dust particles, and ion scattering on dust particles. A new approach to the calculation of particle charges and the screening radius in dense dust clouds is proposed. The results of the simulation are presented, compared with experimental data and discussed. It is demonstrated that for the best agreement between simulated and experimental data, it is necessary to take into account the reflection of electrons from the dust particle surface in order to correctly describe the recombination rate in the cloud, its radial stability, and the dust particle charges.

Development of a high performance (188)W/(188)Re generator by using a synthetic alumina.

PubMed

Lee, Jun Sig; Lee, Jong-Soup; Park, Ul-Jae; Son, Kwang-Jae; Han, Hyon-Soo

2009-01-01

A synthetic alumina functionalized with a sulfate moiety has been developed as the column material of (99)Mo/(99m)Tc and (188)W/(188)Re generators. This material is synthesized by a sol-gel processing. In order to characterize the adsorbent for the (188)W/(188)Re separation, both batch and column contact experiments were conducted. As a result of the experiments, it is found that the maximum capacity of the adsorbent for tungsten is higher than 450mg/g. Hence it is possible to produce approximately 3Ci (188)W/(188)Re generator with only 1g of the adsorbent from (188)W solutions supplied from ORNL, USA or RIAR, Russia. A demonstration study was conducted to show the performance of an (188)W/(188)Re generator column. In this study, 1Ci of (188)W purchased from RIAR, Russia, is loaded on a 0.9cm ID column packed with 0.7g of the adsorbent. Elution of (188)Re is performed every 4-7 days by using the saline solution for more than three months. Nearly 100% of tungsten is loaded by passing 5ml of the (188)W solution (pH=8) through the dry packed column at a 1ml/min flow rate. Elution efficiency of (188)Re is 70-90% by using 5ml of the saline solution. The ratio of (188)W/(188)Re in the eluted solution is 0.002-0.003%. When a Sep-Pak containing 0.26g of acid alumina is installed as a tandem column, the ratio is decreased to less than 10(-3)%. Thin layer chromatography for the eluted (188)Re solution shows 100% radiochemical purity. Also, alumina content in the eluted solution shows less than 10ppm. Through this study, the performance of this adsorbent was successfully demonstrated. By using the developed adsorbent, minimization of the generator column and consequently the volume of eluant could be possible while maintaining the quality of (188)Re just as much as that available in the market.

Highly sensitive determination of alendronate in human plasma and dialysate using metal-free HPLC-MS/MS.

PubMed

Yamada, Miho; Lee, Xiao-Pen; Fujishiro, Masaya; Iseri, Ken; Watanabe, Makoto; Sakamaki, Hiroshi; Uchida, Naoki; Matsuyama, Takaaki; Kumazawa, Takeshi; Takahashi, Haruo; Ishii, Akira; Sato, Keizo

2018-01-01

A highly sensitive method was developed for the analysis of alendronate in human plasma and dialysate using MonoSpin™ SAX ® extraction and metal-free high-performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) following methylation with trimethylsilyldiazomethane. The chromatographic separation of the derivatives for alendronate and alendronate-d 6 was achieved on an L-column2 ODS metal-free column (50 mm  ×  2 mm i.d., particle size 3 µm) with a linear gradient elution system composed of 10 mM ammonium acetate (pH 6.8) and acetonitrile at a flow rate of 0.3 ml/min. Quantification was performed by multiple reaction monitoring (MRM) with positive-ion electrospray ionization (ESI). Distinct peaks were observed for alendronate and for the internal standard on each channel within 1 min. The regression equations showed good linearity within the ranges of 2.0-100 ng/0.5 ml for the plasma and 1.0-100 ng/0.5 ml for the dialysate, with the limits of detection at 1.0 ng/0.5 ml for the plasma and 0.5 ng/0.5 ml for the dialysate. Extraction efficiencies for alendronate for the plasma and dialysate were 41.1-51.2% and 63.6-73.4%, respectively. The coefficient of variation (CV) was ≤8.5%. The method was successfully applied to the analyses of real plasma and dialysate samples derived after intravenous administration of alendronate. Copyright © 2017 Elsevier B.V. All rights reserved.

Gamma source for active interrogation

DOEpatents

Leung, Ka-Ngo; Lou, Tak Pui; Barletta, William A.

2012-10-02

A cylindrical gamma generator includes a coaxial RF-driven plasma ion source and target. A hydrogen plasma is produced by RF excitation in a cylindrical plasma ion generator using an RF antenna. A cylindrical gamma generating target is coaxial with the ion generator, separated by plasma and extraction electrodes which has many openings. The plasma generator emanates ions radially over 360.degree. and the cylindrical target is thus irradiated by ions over its entire circumference. The plasma generator and target may be as long as desired.

Gamma source for active interrogation

DOEpatents

Leung, Ka-Ngo [Hercules, CA; Lou, Tak Pui [Berkeley, CA; Barletta, William A [Oakland, CA

2009-09-29

A cylindrical gamma generator includes a coaxial RF-driven plasma ion source and target. A hydrogen plasma is produced by RF excitation in a cylindrical plasma ion generator using an RF antenna. A cylindrical gamma generating target is coaxial with the ion generator, separated by plasma and extraction electrodes which has many openings. The plasma generator emanates ions radially over 360.degree. and the cylindrical target is thus irradiated by ions over its entire circumference. The plasma generator and target may be as long as desired.

Isolation and structural determination of squalene synthase inhibitor from Prunus mume fruit.

PubMed

Choi, Sung-Won; Hur, Nam-Yoon; Ahn, Soon-Cheol; Kim, Dong-Seob; Lee, Jae-Kwon; Kim, Dae-Ok; Park, Seung-Kook; Kim, Byung-Yong; Baik, Moo-Yeol

2007-12-01

Squalene synthase plays an important role in the cholesterol biosynthetic pathway. Inhibiting this enzyme in hypercholesterolemia can lower not only plasma cholesterol but also plasma triglyceride levels. A squalene synthase inhibitor was screened from Prunus mume fruit, and then purified via sequential processes of ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. The squalene synthase inhibitor was identified as chlorogenic acid with a molecular mass of 354 Da and a molecular formula of C16H18O9 based on UV spectrophotometry, 1H and 13C NMRs, and mass spectrometry. Chlorogenic acid inhibited the squalene synthase of pig liver with an IC50 level of 100 nM. Since chlorogenic acid was an effective inhibitor against the squalene synthase of an animal source, it may be a potential therapeutic agent for hypercholesterolemia.

Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection.

PubMed

Sotgia, Salvatore; Pisanu, Elisabetta; Pintus, Gianfranco; Erre, Gian Luca; Pinna, Gerard Aime; Deiana, Luca; Carru, Ciriaco; Zinellu, Angelo

2013-01-01

Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma. After derivatization of L-ergothioneine with 5-iodoacetamidofluorescein, the separation was carried out by HPLC on an ODS-2 C-18 sperisorb column by using a linear gradient elution and by HPCE on an uncoated fused silica capillary, 50 µm id, and 60 cm length. The methods were validated and found to be linear in the range of 0.3 to 10 µmol/l. The limit of quantification was 0.27 µmol/l for HPCE and 0.15 µmol/l for HPLC. The variations for intra- and inter-assay precision were around 6 RSD%, and the mean recovery accuracy close to 100% (96.11%).

High-performance liquid chromatographic determination of loxoprofen and its diastereomeric alcohol metabolites in biological fluids by fluorescence labelling with 4-bromomethyl-6,7-methylenedioxycoumarin.

PubMed

Naganuma, H; Kawahara, Y

1990-09-14

A simple and sensitive high-performance liquid chromatographic procedure to determine loxoprofen and its diastereomeric alcohol metabolites in biological specimens is described. The analysis involves liquid-liquid extraction with benzene, pre-column derivatization with a highly fluorogenic reagent, 4-bromomethyl-6,7-methylenedioxycoumarin (BrMDC) and subsequent separation on a reversed-phase column. Loxoprofen, its pharmacologically active metabolite, trans-alcohol, and less active cis-alcohol were completely separated within 20 min with a mobile phase of 55% of aqueous acetonitrile containing acetic acid. Any endogenous substances do not interfere in the analysis of either plasma or urine samples. The quantitation limit was 0.01 micrograms/ml for human plasma and 0.05 micrograms/ml for urine. The method was applied to a pharmacokinetic study in healthy human subjects who had received 60 mg of loxoprofen sodium.

Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection

PubMed Central

Sotgia, Salvatore; Pisanu, Elisabetta; Pintus, Gianfranco; Erre, Gian Luca; Pinna, Gerard Aime; Deiana, Luca; Carru, Ciriaco; Zinellu, Angelo

2013-01-01

Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma. After derivatization of L-ergothioneine with 5-iodoacetamidofluorescein, the separation was carried out by HPLC on an ODS-2 C-18 sperisorb column by using a linear gradient elution and by HPCE on an uncoated fused silica capillary, 50 µm id, and 60 cm length. The methods were validated and found to be linear in the range of 0.3 to 10 µmol/l. The limit of quantification was 0.27 µmol/l for HPCE and 0.15 µmol/l for HPLC. The variations for intra- and inter-assay precision were around 6 RSD%, and the mean recovery accuracy close to 100% (96.11%). PMID:23922985

The ability of endotoxin adsorption during a longer duration of direct hemoperfusion with a polymyxin B-immobilized fiber column in patients with septic shock.

PubMed

Shimizu, Tomoharu; Obata, Toru; Sonoda, Hiromichi; Akabori, Hiroya; Tabata, Takahisa; Eguchi, Yutaka; Endo, Yoshihiro; Tani, Tohru

2013-12-01

The patients' hemodynamic conditions of septic shock due to intra-abdominal infection were improved by the longer duration of direct hemoperfusion with a polymyxin B-immobilized fiber column (PMX), reducing plasma endotoxins measured by the novel endotoxin detection method, named endotoxin scattering photometry (ESP) method; however, turbidimetric method could not detect endotoxins. We also observed the reduction in the endotoxin after passing through column by ESP method even after the longer duration of PMX. ESP method may more sensitively detect endotoxins than the ordinary turbidimetric method. Moreover, we demonstrated the ability of endotoxin adsorption in spite of the longer duration of PMX. Copyright © 2013 Elsevier Ltd. All rights reserved.

HPLC-HG-AFS determination of arsenic species in acute promyelocytic leukemia (APL) plasma and blood cells.

PubMed

Guo, Meihua; Wang, Wenjing; Hai, Xin; Zhou, Jin

2017-10-25

Arsenic trioxide (ATO) has been successfully used in the treatment of acute promyelocytic leukemia (APL). To clarify the arsenic species in APL patients, high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) and HG-AFS methods were developed and validated to quantify the plasma concentrations of inorganic arsenic (As(III) and As(V)) and methylated metabolites (MMA and DMA), and the total amounts of arsenic in blood cells and plasma. Blood cells and plasma were digested with mixtures of HNO 3 H 2 O 2 and analyzed by HG-AFS. For arsenic speciation, plasma samples were prepared with perchloric acid to precipitate protein. The supernatant was separated on an anion-exchange column within 6min with isocratic elution using 13mM CH 3 COONa, 3mM NaH 2 PO 4 , 4mM KNO 3 and 0.2mM EDTA-2Na. The methods provided linearity range of 0.2-20ng/mL for total arsenic and 2.0-50ng/mL for four arsenic species. The developed methods for total arsenic and arsenic species determination were precise and accurate. The spiked recoveries ranged from 81.2%-108.6% and the coefficients of variation for intra- and inter-batch precision were less than 9.3% and 12.5%, respectively. The developed methods were applied successfully for the assay of total arsenic and arsenic species in 5 APL patients. The HPLC-HG-AFS may be a good alternative for arsenic species determination in APL patients with its simplicity and low-cost in comparison with HPLC-ICP-MS. Copyright © 2017 Elsevier B.V. All rights reserved.

Relaxation of flux ropes and magnetic reconnection in the Reconnection Scaling Experiment at LANL

NASA Astrophysics Data System (ADS)

Furno, I.; Intrator, T.; Hemsing, E.; Hsu, S.; Lapenta, G.; Abbate, S.

2004-12-01

Magnetic reconnection and plasma relaxation are studied in the Reconnection Scaling Experiment (RSX) with current carrying plasma columns (magnetic flux ropes). Using plasma guns, multiple flux ropes (Bθ ≤ 100 Gauss, L=90 cm, r≤3 cm) are generated in a three-dimensional (3D) cylindrical geometry and are observed to evolve dynamically during the injection of magnetic helicity. Detailed evolution of electron density, temperature, plasma potential and magnetic field structures is reconstructed experimentally and visible light emission is captured with a fast-gated, intensified CCD camera to provide insight into the global flux rope dynamics. Experiments with two flux ropes in collisional plasmas and in a strong axial guide field (Bz / Bθ > 10) suggest that magnetic reconnection plays an important role in the initial stages of flux rope evolution. During the early stages of the applied current drive (t≤ 20 τ Alfv´ {e}n), the flux ropes are observed to twist, partially coalesce and form a thin current sheet with a scale size comparable to that of the ion sound gyro-radius. Here, non-ideal terms in a generalized Ohm's Law appear to play a significant role in the 3D reconnection process as shown by the presence of a strong axial pressure gradient in the current sheet. In addition, a density perturbation with a structure characteristic of a kinetic Alfvén wave is observed to propagate axially in the current layer, anti-parallel to the induced sheet current. Later in the evolution, when a sufficient amount of helicity is injected into the system, a critical threshold for the kink instability is exceeded and the helical twisting of each individual flux rope can dominate the dynamics of the system. This may prevent the complete coalescence of the flux ropes.

Relaxation of flux ropes and magnetic reconnection in the Reconnection Scaling Experiment at LANL

NASA Astrophysics Data System (ADS)

Furno, Ivo

2004-11-01

Magnetic reconnection and plasma relaxation are studied in the Reconnection Scaling Experiment (RSX) with current carrying plasma columns (magnetic flux ropes). Using plasma guns, multiple flux ropes (B_pol < 100 Gauss, L=90 cm, r < 3 cm) are generated in a three-dimensional (3D) cylindrical geometry and are observed to evolve dynamically during the injection of magnetic helicity. Detailed evolution of electron density, temperature, plasma potential and magnetic field structures is reconstructed experimentally and visible light emission is captured with a fast-gated, intensified CCD camera to provide insight into the global flux rope dynamics. Experiments with two flux ropes in collisional plasmas and in a strong axial guide field (Bz / B_pol > 10) suggest that magnetic reconnection plays an important role in the initial stages of flux rope evolution. During the early stages of the applied current drive (t < 20τ_Alfven), the flux ropes are observed to twist, partially coalesce and form a thin current sheet with a scale size comparable to that of the ion sound gyro-radius. Here, non-ideal terms in a generalized Ohm's Law appear to play a significant role in the 3D reconnection process as shown by the presence of a strong axial pressure gradient in the current sheet. In addition, a density perturbation with a structure characteristic of a kinetic Alfvén wave is observed to propagate axially in the current layer, anti-parallel to the induced sheet current. Later in the evolution, when a sufficient amount of helicity is injected into the system, a critical threshold for the kink instability is exceeded and the helical twisting of each individual flux rope can dominate the dynamics of the system. This may prevent the complete coalescence of the flux ropes.

Analysis of oxcarbazepine and the 10-hydroxycarbazepine enantiomers in plasma by LC-MS/MS: application in a pharmacokinetic study.

PubMed

de Jesus Antunes, Natalicia; Wichert-Ana, Lauro; Coelho, Eduardo Barbosa; Della Pasqua, Oscar; Alexandre, Veriano; Takayanagui, Osvaldo Massaiti; Tozatto, Eduardo; Lanchote, Vera Lucia

2013-12-01

Oxcarbazepine is a second-generation antiepileptic drug indicated as monotherapy or adjunctive therapy in the treatment of partial seizures or generalized tonic-clonic seizures in adults and children. It undergoes rapid presystemic reduction with formation of the active metabolite 10-hydroxycarbazepine (MHD), which has a chiral center at position 10, with the enantiomers (S)-(+)- and R-(-)-MHD showing similar antiepileptic effects. This study presents the development and validation of a method of sequential analysis of oxcarbazepine and MHD enantiomers in plasma using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Aliquots of 100 μL of plasma were extracted with a mixture of methyl tert-butyl ether: dichloromethane (2:1). The separation of oxcarbazepine and the MHD enantiomers was obtained on a chiral phase Chiralcel OD-H column, using a mixture of hexane:ethanol:isopropanol (80:15:5, v/v/v) as mobile phase at a flow rate of 1.3 mL/min with a split ratio of 1:5, and quantification was performed by LC-MS/MS. The limit of quantification was 12.5 ng oxcarbazepine and 31.25 ng of each MHD enantiomer/mL of plasma. The method was applied in the study of kinetic disposition of oxcarbazepine and the MHD enantiomers in the steady state after oral administration of 300 mg/12 h oxcarbazepine in a healthy volunteer. The maximum plasma concentration of oxcarbazepine was 1.2 µg/mL at 0.75 h. The kinetic disposition of MHD is enantioselective, with a higher proportion of the S-(+)-MHD enantiomer compared to R-(-)-MHD and an AUC(0-12) S-(+)/R-(-) ratio of 5.44. © 2013 Wiley Periodicals, Inc.

An UPLC-MS/MS method for the quantitation of alectinib in rat plasma.

PubMed

Huang, Xiang-Xin; Li, Yun-Xuan; Li, Xiang-Yu; Hu, Xiao-Xia; Tang, Peng-Fei; Hu, Guo-Xin

2017-01-05

Currently, crizotinib is the first generation drug, which has been used in the treatment of ALK-rearranged non-small cell lung cancer (NSCLC). However, more and more patients are found in crizotinib-resistance. In the last year, alectinib has been approved for treatment of patients with crizotinib-resistance. In this study, we aim to develop and validate a simple, rapid and sensitive tandem mass spectrometry (UHPLC-MS/MS) method for determination of alectinib in rat plasma. Diazepam was chosen as an internal standard (IS). Protein precipitation by acetonitrile was utilized to prepare plasma samples. Chromatographic separation was achieved on a RRHD Eclipse Plus C18 (2.1×50mm, 1.8μ) column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid). The analytes were detected by an electrospray ionization (ESI) source in positive mode. A dynamic multiple reaction monitoring (MRM) method was developed to detect specific precursor and product ions. The target fragment ions were m/z 483.2→396.1 for alectinib and m/z 285.0→192.9 for diazepam (IS). Linear calibration plots were achieved in the range of 1-500ng/ml for alectinib (R 2 =0.997) in rat plasma. Mean recoveries of alectinib in rat plasma ranged from 84.2% to 92.2%. The intra- and inter-day precision was below 9.3% and accuracy was from -1.4% to 12.1%. No obvious matrix effect was found. This method shows a good performance: accuracy, precision and stability. It has been fully validated and successfully applied to pharmacokinetic study of alectinib. Copyright © 2016 Elsevier B.V. All rights reserved.

Development of very compact soft X-ray lasers

NASA Astrophysics Data System (ADS)

Korobkin, Dmitriy Vladlenovich

1999-10-01

A powerful subpicosecond laser system, based on solid state (Ti:Al 2O3) front end and gas excimer (KrF*) amplifiers, has been developed. It is capable of producing 40-50 mJ pulses at 248 nm (285 fs pulse duration) with 2 Hz repetition rate. That radiation can be focused to intensities greater than 1017 W/cm2 and cause the optical field ionization of lithium. The system was used in experiments on gain generation in hydrogen-like Li III. Lasing action in hydrogen-like Li III 2-1 transition to ground state (13.5 nm) has been demonstrated for the first time. Gain G = 11 cm-1 in 5-mm long plasma column, created in LiF microcapillary, was measured. The observed gain-length product was GL = 5.5. In another set of experiments an inversion population between levels n = 3 and n = 2 in hydrogen-like BV was created using a low-energy compact laser system at 1 Hz repetition rate. Gain G = 17 cm-1 at 26.2 nm was measured in 3 mm long B2O3 microcapillary, which corresponds to GL = 5.1. The entire experimental setup can be fit on a single medium size optical table. Also propagation of laser radiation through plasma, created in microcapillaries has been investigated at various experimental conditions. Real-time plasma probing with low intensity HeNe laser beam has been performed.

Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry

PubMed Central

Jain, Lokesh; Gardner, Erin R.; Venitz, Jürgen; Giaccone, Giuseppe; Houk, Brett E.; Figg, William D.

2010-01-01

A simple, rapid and sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) analytical method was developed for quantification of Hsp90 inhibitor PF-04928473 in human plasma, following administration of its prodrug, PF-04929113. Sample processing involved protein precipitation by addition of 0.4 mL of methanol containing internal standard (PF-04972487) to 50 μL volume of plasma sample. Chromatographic separation of PF-04928473 and PF-04972487 was achieved on a Phenomenex® Luna C18(2) (2.0×50 mm, 5 μm) column using a gradient elution method with mobile phase solvents: methanol containing 0.1% formic acid and 0.1% formic acid at a flow rate of 0.25 mL/min. Detection was performed in electrospray positive ionization mode, monitoring the ion transitions from m/z 465.1→350.1 (PF-04928473) and m/z 447.0→329.1 (PF-04972487). The retention times for PF-04928473 and PF-04972487 were 1.86 and 2.85 minutes, respectively. Calibration curves were generated in the range of 2–2000 ng/mL. The accuracy and precision ranged from 94.1–99.0% and 86.7–97.6%, respectively, which were calculated using quality control samples of three different concentrations analyzed in quintuplicate on four different days. PMID:20951100

Determination of bupropion using liquid chromatography with fluorescence detection in pharmaceutical preparations, human plasma and human urine.

PubMed

Ulu, Sevgi Tatar; Tuncel, Muzaffer

2012-05-01

A novel pre-column derivatization reversed-phase high-performance liquid chromatography with fluorescence detection is described for the determination of bupropion in pharmaceutical preparation, human plasma and human urine using mexiletine as internal standard. The proposed method is based on the reaction of 4-chloro-7-nitrobenzofurazan (NBD-Cl) with bupropion to produce a fluorescent derivative. The derivative formed is monitored on a C18 (150 mm × 4.6 mm i.d., 5 µm) column using a mobile phase consisting of methanol-water 75:25 (v/v), at a flow-rate of 1.2 mL/min and detected fluorimetrically at λ(ex) = 458 and λ(em) = 533 nm. The assay was linear over the concentration ranges of 5-500 and 10-500 ng/mL for plasma and urine, respectively. The limits of detection and quantification were calculated to be 0.24 and 0.72 ng/mL for plasma and urine, respectively (inter-day results). The recoveries obtained for plasma and urine were 97.12% ± 0.45 and 96.00% ± 0.45, respectively. The method presents good performance in terms of precision, accuracy, specificity, linearity, detection and quantification limits and robustness. The proposed method is applied to determine bupropion in commercially available tablets. The results were compared with an ultraviolet spectrophotometry method using t- and F-tests. © The Author [2012]. Published by Oxford University Press. All rights reserved.

Novel liquid chromatographic assay for the low-level determination of apomorphine in plasma.

PubMed

Priston, M J; Sewell, G J

1996-05-31

A novel HPLC assay which is rapid, reproducible and sensitive has been developed for the analysis of apomorphine in plasma. The assay incorporates boldine as an internal standard, and uses solid-phase extraction on C18 mini-columns for sample clean-up and concentration, so enabling quantitation of apomorphine at 500 pg/ml using fluorescence detection (lambda(ex) 270 nm, lambda(em) 450 nm). The HPLC assay comprised a 25 cm-long Techopak C18 column and a mobile phase of (0.25 M sodium dihydrogen phosphate plus 0.25% heptane sulphonic acid, to pH 3.3 with orthophosphoric acid) containing 30% (v/v) methanol and 0.003% (w/v) EDTA, run at a flow-rate of 1.5 ml/min. Calibration plots prepared in plasma were linear over the range 1-30 ng/ml, (limit of quantitation (LOQ) = 490 pg/ml) with R.S.D. of 0.05% and R.E. of 5.0% at the level of 1 ng/ml. Preliminary pharmacokinetic data from two patients given apomorphine by 12 h subcutaneous infusion (patient A dose = 35 mg and patient B dose = 141 mg) showed apomorphine elimination from plasma to fit a two-compartment model, with initial half-lives of 8.2 and 46.6 min, elimination half-lives of 76.4 and 166.5 min and area under the plasma concentration-time curve (AUC) values of 236 and 405 ng h/ml, respectively.

Comparison of protocols and RNA carriers for plasma miRNA isolation. Unraveling RNA carrier influence on miRNA isolation

PubMed Central

Martos, Laura; Fernández-Pardo, Álvaro; Oto, Julia; Medina, Pilar; España, Francisco; Navarro, Silvia

2017-01-01

microRNAs are promising biomarkers in biological fluids in several diseases. Different plasma RNA isolation protocols and carriers are available, but their efficiencies have been scarcely compared. Plasma microRNAs were isolated using a phenol and column-based procedure and a column-based procedure, in the presence or absence of two RNA carriers (yeast RNA and MS2 RNA). We evaluated the presence of PCR inhibitors and the relative abundance of certain microRNAs by qRT-PCR. Furthermore, we analyzed the association between different isolation protocols, the relative abundance of the miRNAs in the sample, the GC content and the free energy of microRNAs. In all microRNAs analyzed, the addition of yeast RNA as a carrier in the different isolation protocols used gave lower raw Cq values, indicating higher microRNA recovery. Moreover, this increase in microRNAs recovery was dependent on their own relative abundance in the sample, their GC content and the free-energy of their own most stable secondary structure. Furthermore, the normalization of microRNA levels by an endogenous microRNA is more reliable than the normalization by plasma volume, as it reduced the difference in microRNA fold abundance between the different isolation protocols evaluated. Our thorough study indicates that a standardization of pre- and analytical conditions is necessary to obtain reproducible inter-laboratory results in plasma microRNA studies. PMID:29077772

Swirl Ring Improves Performance Of Welding Torch

NASA Technical Reports Server (NTRS)

Mcgee, William F.; Rybicki, Daniel J.

1995-01-01

Plasma-arc welding torch modified to create vortex in plasma gas to focus arc into narrower and denser column. Swirl ring contains four channels with angled exit holes to force gas to swirl as it flows out of torch past tip of electrode. Degradation of electrode and orifice more uniform and need to rotate torch during operation to compensate for asymmetry in arc reduced or eliminated. Used in both keyhole and nonkeyhole welding modes.

Unsteady Plasma Ejections from Hollow Accretion Columns of Galactic Neutron Stars as a Trigger for Gamma-Ray Bursts

NASA Astrophysics Data System (ADS)

Gvaramadze, V. V.

1995-09-01

We propose a model of gamma-ray bursts (GRBs) based on close Galactic neutron stars with accretion disks. We outline a simple mechanism of unsteady plasma ejections during episodic accretion events. The relative kinetic energy of ejected blobs can be converted into gamma-rays by internal shocks. The beaming of gamma-ray emission can be responsible for the observed isotropic angular distribution of GRBs.

UPLC-MRM Mass Spectrometry Method for Measurement of the Coagulation Inhibitors Dabigatran and Rivaroxaban in Human Plasma and Its Comparison with Functional Assays.

PubMed

Kuhn, Joachim; Gripp, Tatjana; Flieder, Tobias; Dittrich, Marcus; Hendig, Doris; Busse, Jessica; Knabbe, Cornelius; Birschmann, Ingvild

2015-01-01

The fast, precise, and accurate measurement of the new generation of oral anticoagulants such as dabigatran and rivaroxaban in patients' plasma my provide important information in different clinical circumstances such as in the case of suspicion of overdose, when patients switch from existing oral anticoagulant, in patients with hepatic or renal impairment, by concomitant use of interaction drugs, or to assess anticoagulant concentration in patients' blood before major surgery. Here, we describe a quick and precise method to measure the coagulation inhibitors dabigatran and rivaroxaban using ultra-performance liquid chromatography electrospray ionization-tandem mass spectrometry in multiple reactions monitoring (MRM) mode (UPLC-MRM MS). Internal standards (ISs) were added to the sample and after protein precipitation; the sample was separated on a reverse phase column. After ionization of the analytes the ions were detected using electrospray ionization-tandem mass spectrometry. Run time was 2.5 minutes per injection. Ion suppression was characterized by means of post-column infusion. The calibration curves of dabigatran and rivaroxaban were linear over the working range between 0.8 and 800 μg/L (r >0.99). Limits of detection (LOD) in the plasma matrix were 0.21 μg/L for dabigatran and 0.34 μg/L for rivaroxaban, and lower limits of quantification (LLOQ) in the plasma matrix were 0.46 μg/L for dabigatran and 0.54 μg/L for rivaroxaban. The intraassay coefficients of variation (CVs) for dabigatran and rivaroxaban were < 4% and 6%; respectively, the interassay CVs were < 6% for dabigatran and < 9% for rivaroxaban. Inaccuracy was < 5% for both substances. The mean recovery was 104.5% (range 83.8-113.0%) for dabigatran and 87.0% (range 73.6-105.4%) for rivaroxaban. No significant ion suppressions were detected at the elution times of dabigatran or rivaroxaban. Both coagulation inhibitors were stable in citrate plasma at -20°C, 4°C and even at RT for at least one week. A method comparison between our UPLC-MRM MS method, the commercially available automated Direct Thrombin Inhibitor assay (DTI assay) for dabigatran measurement from CoaChrom Diagnostica, as well as the automated anti-Xa assay for rivaroxaban measurement from Chromogenix both performed by ACL-TOP showed a high degree of correlation. However, UPLC-MRM MS measurement of dabigatran and rivaroxaban has a much better selectivity than classical functional assays measuring activities of various coagulation factors which are susceptible to interference by other coagulant drugs. Overall, we developed and validated a sensitive and specific UPLC-MRM MS assay for the quick and specific measurement of dabigatran and rivaroxaban in human plasma.

UPLC-MRM Mass Spectrometry Method for Measurement of the Coagulation Inhibitors Dabigatran and Rivaroxaban in Human Plasma and Its Comparison with Functional Assays

PubMed Central

Kuhn, Joachim; Gripp, Tatjana; Flieder, Tobias; Dittrich, Marcus; Hendig, Doris; Busse, Jessica; Knabbe, Cornelius; Birschmann, Ingvild

2015-01-01

Introduction The fast, precise, and accurate measurement of the new generation of oral anticoagulants such as dabigatran and rivaroxaban in patients’ plasma my provide important information in different clinical circumstances such as in the case of suspicion of overdose, when patients switch from existing oral anticoagulant, in patients with hepatic or renal impairment, by concomitant use of interaction drugs, or to assess anticoagulant concentration in patients’ blood before major surgery. Methods Here, we describe a quick and precise method to measure the coagulation inhibitors dabigatran and rivaroxaban using ultra-performance liquid chromatography electrospray ionization-tandem mass spectrometry in multiple reactions monitoring (MRM) mode (UPLC-MRM MS). Internal standards (ISs) were added to the sample and after protein precipitation; the sample was separated on a reverse phase column. After ionization of the analytes the ions were detected using electrospray ionization-tandem mass spectrometry. Run time was 2.5 minutes per injection. Ion suppression was characterized by means of post-column infusion. Results The calibration curves of dabigatran and rivaroxaban were linear over the working range between 0.8 and 800 μg/L (r >0.99). Limits of detection (LOD) in the plasma matrix were 0.21 μg/L for dabigatran and 0.34 μg/L for rivaroxaban, and lower limits of quantification (LLOQ) in the plasma matrix were 0.46 μg/L for dabigatran and 0.54 μg/L for rivaroxaban. The intraassay coefficients of variation (CVs) for dabigatran and rivaroxaban were < 4% and 6%; respectively, the interassay CVs were < 6% for dabigatran and < 9% for rivaroxaban. Inaccuracy was < 5% for both substances. The mean recovery was 104.5% (range 83.8–113.0%) for dabigatran and 87.0% (range 73.6–105.4%) for rivaroxaban. No significant ion suppressions were detected at the elution times of dabigatran or rivaroxaban. Both coagulation inhibitors were stable in citrate plasma at -20°C, 4°C and even at RT for at least one week. A method comparison between our UPLC-MRM MS method, the commercially available automated Direct Thrombin Inhibitor assay (DTI assay) for dabigatran measurement from CoaChrom Diagnostica, as well as the automated anti-Xa assay for rivaroxaban measurement from Chromogenix both performed by ACL-TOP showed a high degree of correlation. However, UPLC-MRM MS measurement of dabigatran and rivaroxaban has a much better selectivity than classical functional assays measuring activities of various coagulation factors which are susceptible to interference by other coagulant drugs. Conclusions Overall, we developed and validated a sensitive and specific UPLC-MRM MS assay for the quick and specific measurement of dabigatran and rivaroxaban in human plasma. PMID:26699714

X-ray flares from runaway pair production in active galactic nuclei

NASA Technical Reports Server (NTRS)

Kirk, J. G.; Mastichiadis, A.

1992-01-01

The hard X-ray spectrum of AGNs is nonthermal, probably arising from an electron-positron pair cascade, with some emission reflected off relatively cold matter. There has been interest in models on which protons are accelerated and create relativistic electrons on interaction with a local radiation field. It is shown here that a sufficient column density of protons can lead to runaway pair production: photons generated by the relativistic pairs are the targets for the protons to produce more pairs. This can produce X-ray fluxes with the characteristics observed in AGN. The model predicts the maximum ratio of luminosity to source size as well as their spectrum in the early phases. The same mechanism may also be able to create the knots of synchrotron-radiating pair plasma seen in sources such as 3C273.

Vessel structural support system

DOEpatents

Jenko, James X.; Ott, Howard L.; Wilson, Robert M.; Wepfer, Robert M.

1992-01-01

Vessel structural support system for laterally and vertically supporting a vessel, such as a nuclear steam generator having an exterior bottom surface and a side surface thereon. The system includes a bracket connected to the bottom surface. A support column is pivotally connected to the bracket for vertically supporting the steam generator. The system also includes a base pad assembly connected pivotally to the support column for supporting the support column and the steam generator. The base pad assembly, which is capable of being brought to a level position by turning leveling nuts, is anchored to a floor. The system further includes a male key member attached to the side surface of the steam generator and a female stop member attached to an adjacent wall. The male key member and the female stop member coact to laterally support the steam generator. Moreover, the system includes a snubber assembly connected to the side surface of the steam generator and also attached to the adjacent wall for dampening lateral movement of the steam generator. In addition, the system includes a restraining member of "flat" attached to the side surface of the steam generator and a bumper attached to the adjacent wall. The flat and the bumper coact to further laterally support the steam generator.

HPLC-electrospray mass spectrometric assay for the determination of (R,R)-fenoterol in rat plasma.

PubMed

Siluk, Danuta; Kim, Hee Seung; Cole, Tyler; Wainer, Irving W

2008-11-04

A fast and specific liquid chromatography-mass spectrometry method for the determination of (R,R)-fenoterol ((R,R)-Fen) in rat plasma has been developed and validated. (R,R)-Fen was extracted from 125 microl of plasma using solid phase extraction and analyzed on Atlantis HILIC Silica 3 microm column. The mobile phase was composed of acetonitrile:ammonium acetate (pH 4.1; 20mM) (85:15, v/v), at a flow rate of 0.2 ml/min. The lower limit of detection (LLOD) was 2 ng/ml . The procedure was validated and applied to the analysis of plasma samples from rats previously administered (R,R)-Fen in an intravenous bolus.

Plasma volume methodology: Evans blue, hemoglobin-hematocrit, and mass density transformations

NASA Technical Reports Server (NTRS)

Greenleaf, J. E.; Hinghofer-Szalkay, H.

1985-01-01

Methods for measuring absolute levels and changes in plasma volume are presented along with derivations of pertinent equations. Reduction in variability of the Evans blue dye dilution technique using chromatographic column purification suggests that the day-to-day variability in the plasma volume in humans is less than + or - 20 m1. Mass density determination using the mechanical-oscillator technique provides a method for measuring vascular fluid shifts continuously for assessing the density of the filtrate, and for quantifying movements of protein across microvascular walls. Equations for the calculation of volume and density of shifted fluid are presented.

Influence of Magnetic Field Ripple on the Intrinsic Rotation of Tokamak Plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nave, M. F. F.; Johnson, T.; Eriksson, L.-G.

Using the unique capability of JET to monotonically change the amplitude of the magnetic field ripple, without modifying other relevant equilibrium conditions, the effect of the ripple on the angular rotation frequency of the plasma column was investigated under the conditions of no external momentum input. The ripple amplitude was varied from 0.08% to 1.5% in Ohmic and ion-cyclotron radio-frequency (ICRF) heated plasmas. In both cases the ripple causes counterrotation, indicating a strong torque due to nonambipolar transport of thermal ions and in the case of ICRF also fast ions.

Plasma physics goes beyond fusion

NASA Astrophysics Data System (ADS)

Franklin, Raoul

2008-11-01

I was interested to read the fusion supplement published with the October issue of Physics World. However, in asserting that fusion created the need to recognize plasma physics as a separate branch of the subject, Stephen Cowley, the new director of the United Kingdom Atomic Energy Authority, was not quite correct. In fact, the word "plasma" was appropriated from the Greek by the chemical physicist (and later Nobel laureate) Irving Langmuir in 1928. It was used to describe the positive column of a gas discharge, which was then the subject of research into better lighting sources and advertising displays, as well as the underlying science.

Fibulin-1 purification from human plasma using affinity chromatography on Factor H-Sepharose

PubMed Central

DiScipio, Richard G.; Liddington, Robert C.; Schraufstatter, Ingrid U.

2016-01-01

A method is reported to purify Fibulin-1 from human plasma resulting in a 36% recovery. The steps involve removal of the cryoglobulin and the vitamin K dependent proteins followed by polyethylene glycol and ammonium sulfate precipitations, DEAE-Sephadex column chromatography and finally Factor H-Sepharose affinity purification. The procedure is designed to be integrated into an overall scheme for the isolation of over 30 plasma proteins from a single batch of human plasma. Results from mass spectroscopy, SDS-PAGE, and Western blotting indicate that human plasma Fibulin-1 is a single chain of the largest isotype. Functional binding assays demonstrated calcium ion dependent interaction of Fibulin-1 for fibrinogen, fibronectin, and Factor H. The procedure described is the first to our knowledge that enables a large scale purification of Fibulin-1 from human plasma. PMID:26826315

Use of vacuum arc plasma guns for a metal puff Z-pinch system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rousskikh, A. G.; Zhigalin, A. S.; Oreshkin, V. I.

The performance of a metal puff Z-pinch system has been studied experimentally. In this type of system, the initial cylindrical shell 4 cm in diameter was produced by ten plasma guns. Each gun initiates a vacuum arc operating between magnesium electrodes. The net current of the guns was 80 kA. The arc-produced plasma shell was compressed by using a 450-kA, 450-ns driver, and as a result, a plasma column 0.3 cm in diameter was formed. The electron temperature of the plasma reached 400 eV at an average ion concentration of 1.85 {center_dot} 10{sup 18} cm{sup -3}. The power of themore » Mg K-line radiation emitted by the plasma for 15-30 ns was 300 MW/cm.« less

Characterizing pressure issues due to turbulent flow in tubing, in ultra-fast chiral supercritical fluid chromatography at up to 580bar.

PubMed

Berger, Terry A

2016-12-02

It has been widely suggested that the outlet pressure be changed to maintain constant density ("isopycnic" conditions) when comparing the kinetic performance of different columns in supercritical fluid chromatography (SFC). However, at high flow rates, flow in the tubing is turbulent, causing large extra-column pressure drops that limit options for changing outlet pressure. Some of these pressure drops occur before and some after the column, obscuring the actual column inlet and outlet pressures. In this work, a 4.6×100mm, 1.8μm R,R-Whelk-O1 column was used with low dispersion LD (120μm) plumbing to generate sub-1min chiral separations. However, the optimum, or near optimum, flow rate was 5mL-min -1 , producing a system pressure of 580bar (with 40% methanol, outlet pressure 120bar). Both the flow rate and pump pressure required were near the limits of the instrument, and significantly exceeded the capability of many other SFC's. Extra-column pressure drops (ΔP ec ) were as high as 200bar, caused mostly by turbulent flow in the tubing. The ΔP ec increased by more than the square of the flow rate. Reynolds Numbers (Re) were calculated for tubing as a function of flow rate between 100 and 400bar and 5-20% methanol in CO 2 , and 40°-60°C. This represents the most extensive analysis of turbulence in tubing in the SFC literature. Flow in 120μm ID tubing was calculated to be laminar below 1.0mL-min -1 , mostly transitional up to 2.5mL-min -1 and virtually always turbulent at 3mL-min -1 and higher. Flow in 170μm tubing is turbulent at lower flows but generates half the ΔP ec due to the lower mobile phase linear velocity. The results suggest that, while sub-minute chromatograms are easily generated, 4.6mm columns are not very user friendly for use with sub-2μm packings. The high flow rates required just to reach optimum result in high ΔP ec generated by the tubing, causing uncertainty in the true column inlet, outlet, and average column pressure/density. When comparing kinetic performance of columns with different dimensions, the pressure drops in the tubing must be considered. Copyright © 2016 Elsevier B.V. All rights reserved.

Trace determination of lenalidomide in plasma by non-extractive HPLC procedures with fluorescence detection after pre-column derivatization with fluorescamine

PubMed Central

2013-01-01

Background Lenalidomide (LND) is a new potent drug used for treatment of multiple myeloma. For its pharmacokinetic studies and therapeutic monitoring, a proper analytical method was required. Results In this study, a non extractive and simple pre-column derivatization procedures have been proposed, for the for trace determination of lenalidomide (LND) in human plasma by HPLC with fluorescence detection. Plasma samples were treated with acetonitrile for protein precipitation then treated with copper acetate to form stable complexes with the biogenic amines and mask their interference with the derivatization reaction of LND. Treated plasma samples containing LND was derivatized with fluorescamine (FLC) in aqueous media at ambient temperature. Separation of the derivatized LND was performed on Hypersil BDS C18 column (250 × 4.6 mm, 5 μm particle size) using a mobile phase consisting of phosphate buffer (pH 4):methanol: tetrahydrofuran (70:10:20, v/v) at a flow rate of 1.0 mL/min. The derivatized samples were monitored at an emission wavelength of 495 nm after excitation at a wavelength of 382 nm. Under the optimum chromatographic conditions, a linear relationship with good correlation coefficient (r = 0.9997, n = 9) was found between the peak area and LND concentrations in the range of 2–100 ng/mL. The limits of detection and quantitation were 0.8 and 2.30 ng/mL, respectively. The intra- and inter-assay precisions were satisfactory and the accuracy of the method was proved. The recovery of LND from the spiked human plasma was 99.30 ± 2.88. Conclusions The proposed method had high throughput as the analysis involved simple sample pre-treatment procedure and a relatively short run-time (< 15 min). The results demonstrated that the method would have a great value when it is applied in the therapeutic monitoring and pharmacokinetic studies for LND. PMID:23497635

Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.

PubMed

Ling, Xu; Zou, Li; Chen, Zilin

2017-09-01

A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed. The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length). The column was then applied for the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae. Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C 18 separation column and UV detection. The effects of sample flow rate, sample volume, and pH value were optimized. The method showed low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen. Finally the method was successfully applied to the determination of psoralen and isopsoralen in spiked herb extracts and rat plasma where it gave recoveries that ranged between 93.2 and 102.1%. The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Analysis of programming properties and the row-column generation method for 1-norm support vector machines.

PubMed

Zhang, Li; Zhou, WeiDa

2013-12-01

This paper deals with fast methods for training a 1-norm support vector machine (SVM). First, we define a specific class of linear programming with many sparse constraints, i.e., row-column sparse constraint linear programming (RCSC-LP). In nature, the 1-norm SVM is a sort of RCSC-LP. In order to construct subproblems for RCSC-LP and solve them, a family of row-column generation (RCG) methods is introduced. RCG methods belong to a category of decomposition techniques, and perform row and column generations in a parallel fashion. Specially, for the 1-norm SVM, the maximum size of subproblems of RCG is identical with the number of Support Vectors (SVs). We also introduce a semi-deleting rule for RCG methods and prove the convergence of RCG methods when using the semi-deleting rule. Experimental results on toy data and real-world datasets illustrate that it is efficient to use RCG to train the 1-norm SVM, especially in the case of small SVs. Copyright © 2013 Elsevier Ltd. All rights reserved.

Generating moment matching scenarios using optimization techniques

DOE PAGES

Mehrotra, Sanjay; Papp, Dávid

2013-05-16

An optimization based method is proposed to generate moment matching scenarios for numerical integration and its use in stochastic programming. The main advantage of the method is its flexibility: it can generate scenarios matching any prescribed set of moments of the underlying distribution rather than matching all moments up to a certain order, and the distribution can be defined over an arbitrary set. This allows for a reduction in the number of scenarios and allows the scenarios to be better tailored to the problem at hand. The method is based on a semi-infinite linear programming formulation of the problem thatmore » is shown to be solvable with polynomial iteration complexity. A practical column generation method is implemented. The column generation subproblems are polynomial optimization problems; however, they need not be solved to optimality. It is found that the columns in the column generation approach can be efficiently generated by random sampling. The number of scenarios generated matches a lower bound of Tchakaloff's. The rate of convergence of the approximation error is established for continuous integrands, and an improved bound is given for smooth integrands. Extensive numerical experiments are presented in which variants of the proposed method are compared to Monte Carlo and quasi-Monte Carlo methods on both numerical integration problems and stochastic optimization problems. The benefits of being able to match any prescribed set of moments, rather than all moments up to a certain order, is also demonstrated using optimization problems with 100-dimensional random vectors. Here, empirical results show that the proposed approach outperforms Monte Carlo and quasi-Monte Carlo based approaches on the tested problems.« less

Determination and pharmacokinetic properties of arsenic speciation in Xiao-Er-Zhi-Bao-Wan by high-performance liquid chromatography with inductively coupled plasma mass spectrometry.

PubMed

Han, Xu; Luo, Jiaoyang; Zhou, Wenju; Yang, Shihai; Yang, Meihua

2016-10-01

A method of high performance liquid chromatography with a Hamilton PRP-X100 ion-exchange column (250 × 4.1 mm id, 10 μm) coupled to inductively coupled plasma mass spectrometry was employed to generate a full concentration-time profile of arsenic speciation after oral administration. The results exhibited good linearity and revealed that, in the pills, the average arsenic concentration was 10105.4 ± 380.7 mg/kg, and in the water extraction solution, the inorganic As(III) and As(V) concentrations were 220.1 ± 12.6 and 45.5 ± 2.3 mg/kg, respectively. No trace of monomethyl arsenic acid was detected in any of the plasma samples. We then successfully applied the established methodology to examine the pharmacokinetics of arsenic speciation. The resulting data revealed that, after oral administration in rats, the plasma concentration of each arsenic species reached C max shortly after initial dosing, and that the distribution and elimination of As(V) was faster than that of As(III) and dimethyl arsenic acid. Additionally, the t 1/2 values of As(V), As(III), and dimethyl arsenic acid were 3.4 ± 1.6, 14.3 ± 4.0, and 19.9 ± 1.6 h, respectively. This study provides references for the determination of arsenic speciation in mineral-containing medicines and could serve as a useful tool in measuring the true toxicity in traditional medicines that contain them. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of dienogest in human plasma and its pharmacokinetic applications under fasting.

PubMed

Pallapothu, Leela Mohan Kumar; Batta, Neelima; Pigili, Ravi Kumar; Yejella, Rajendra Prasad

2015-02-01

A simple, rapid and sensitive analytical method using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) detection with positive ion electrospray ionization was developed for the determination of dienogest in human K2 EDTA plasma using levonorgestrel d6 as an internal standard (IS). Dienogest and IS were extracted from human plasma using simple liquid-liquid extraction. Chromatographic separation was achieved on a Zorbax XDB-Phenyl column (4.6 × 75 mm, 3.5 µm) under isocratic conditions using acetonitrile-5 mm ammonium acetate (70:30, v/v) at a flow rate of 0.60 mL/min. The protonated precursor to product ion transitions monitored for dienogest and IS were at m/z 312.30 → 135.30 and 319.00 → 251.30, respectively. The method was validated with a linearity range of 1.003-200.896 ng/mL having a total analysis time for each chromatograph of 3.0 min. The method has shown tremendous reproducibility with intra- and inter-day precision (coefficient of variation) <3.97 and 6.10%, respectively, and accuracy within ±4.0% of nominal values. The validated method was applied to a pharmacokinetic study in human plasma samples generated after administration of a single oral dose of 2.0 mg dienogest tablets to healthy female volunteers and was proved to be highly reliable for the analysis of clinical samples. Copyright © 2014 John Wiley & Sons, Ltd.

Quantitative method for the determination of Iso-fludelone (KOS-1803) in human plasma by LC-MS/MS

PubMed Central

Christner, Susan M.; Parise, Robert A.; Levine, Erica D.; Rizvi, Naiyer A.; Gounder, Mrinal M.; Beumer, Jan H.

2014-01-01

Epothilones are relatively new tubulin-poison anticancer drugs. Iso-fludelone (KOS-1803) is a synthetic third generation epothilone drug discovered at Memorial Sloan Kettering Cancer Center, and currently in Phase I clinical trials. We report an LC-MS/MS assay for the sensitive, accurate and precise quantitation of Iso-fludelone in 0.2 mL of human plasma. Validation was performed according to FDA guidance. The assay comprised of KOS-1724 as the internal standard and an MTBE liquid-liquid extraction with a water wash step. Separation was achieved with an YMC-Pack ODS-AQ column and an isocratic mobile phase of 0.1% formic acid in acetonitrile and water (70:30, v/v) at 0.3 mL/min for 4 min. Chromatographic separation was followed by electrospray, positive-mode ionization tandem mass spectrometric detection in the multiple reaction monitoring (MRM) mode. The assay was linear from 0.1– 300 ng/mL and was accurate (−9.41–7.07%) and precise (1.03–13.7%) which fulfilled FDA criteria for validation. Recovery from plasma was 73.9–79.7% and ion suppression was negligible (−22.8 to −31.3%). Plasma freeze thaw stability (99.97–105.7%), stability for 11 months at −80 °C (94.93–107.9%), and stability for 6 h at room temperature (94.75–105.5%) were all acceptable. This assay is currently being applied to quantitate Iso-fludelone in clinical samples. PMID:25168219

Quantitative method for the determination of iso-fludelone (KOS-1803) in human plasma by LC-MS/MS.

PubMed

Christner, Susan M; Parise, Robert A; Levine, Erica D; Rizvi, Naiyer A; Gounder, Mrinal M; Beumer, Jan H

2014-11-01

Epothilones are relatively new tubulin-poison anticancer drugs. Iso-fludelone (KOS-1803) is a synthetic third generation epothilone drug discovered at Memorial Sloan Kettering Cancer Center, and currently in phase I clinical trials. We report an LC-MS/MS assay for the sensitive, accurate and precise quantitation of iso-fludelone in 0.2mL of human plasma. Validation was performed according to FDA guidance. The assay comprised of KOS-1724 as the internal standard and an MTBE liquid-liquid extraction with a water wash step. Separation was achieved with an YMC-Pack ODS-AQ column and an isocratic mobile phase of 0.1% formic acid in acetonitrile and water (70:30, v/v) at 0.3mL/min for 4min. Chromatographic separation was followed by electrospray, positive-mode ionization tandem mass spectrometric detection in the multiple reaction monitoring (MRM) mode. The assay was linear from 0.1 to 300ng/mL and was accurate (-9.41 to -7.07%) and precise (1.03-13.7%) which fulfilled FDA criteria for validation. Recovery from plasma was 73.9-79.7% and ion suppression was negligible (-22.8 to -31.3%). Plasma freeze-thaw stability (99.97-105.7%), stability for 11 months at -80°C (94.93-107.9%), and stability for 6h at room temperature (94.75-105.5%) were all acceptable. This assay is currently being applied to quantitate iso-fludelone in clinical samples. Copyright © 2014 Elsevier B.V. All rights reserved.

A theoretical and experimental investigation of the linear and nonlinear impulse responses from a magnetoplasma column

NASA Technical Reports Server (NTRS)

Grody, N. C.

1973-01-01

Linear and nonlinear responses of a magnetoplasma resulting from inhomogeneity in the background plasma density are studied. The plasma response to an impulse electric field was measured and the results are compared with the theory of an inhomogeneous cold plasma. Impulse responses were recorded for the different plasma densities, static magnetic fields, and neutral pressures and generally appeared as modulated, damped oscillations. The frequency spectra of the waveforms consisted of two separated resonance peaks. For weak excitation, the results correlate with the linear theory of a cold, inhomogeneous, cylindrical magnetoplasma. The damping mechanism is identified with that of phase mixing due to inhomogeneity in plasma density. With increasing excitation voltage, the nonlinear impulse responses display stronger damping and a small increase in the frequency of oscillation.

Magnetic confinement of weakly ionized plasma with superconducting bulk magnets

NASA Astrophysics Data System (ADS)

Matsuzawa, Hidenori; Ohishi, Kazuya; Ishikawa, Kazuhito; Morita, Tomonori; Yoshikawa, Masaaki; Ikuta, Hiroshi; Mizutani, Uichiro

2003-04-01

This letter describes the application of single-domain superconducting bulk magnets as a plasma confinement. A through-hole was drilled at the center of a Sm123 bulk superconductor of 39 mm diameter and 17 mm thickness. When the sample was field cooled to 77 K, the resulting bulk magnet trapped a magnetic field of ˜0.65 T called a magnetic mirror, in the bore of the hole. The magnet was applied to a weakly ionized neon plasma column. Both the magnet and discharge glass tube were immersed in liquid nitrogen. The spatial distribution in the tube of red fluorescence of the plasma showed that the magnet certainly confined the plasma. These results would provide a clue to applications of the compact magnet of strong magnetic field.

Study of array plasma antenna parameters

NASA Astrophysics Data System (ADS)

Kumar, Rajneesh; Kumar, Prince

2018-04-01

This paper is aimed to investigate the array plasma antenna parameters to help the optimization of an array plasma antenna. Single plasma antenna is transformed into array plasma antenna by changing the operating parameters. The re-configurability arises in the form of striations, due to transverse bifurcation of plasma column by changing the operating parameters. Each striation can be treated as an antenna element and system performs like an array plasma antenna. In order to achieve the goal of this paper, three different configurations of array plasma antenna (namely Array 1, Array 2 and Array 3) are simulated. The observations are made on variation in antenna parameters like resonance frequency, radiation pattern, directivity and gain with variation in length and number of antenna elements for each array plasma antenna. Moreover experiments are also performed and results are compared with simulation. Further array plasma antenna parameters are also compared with monopole plasma antenna parameters. The study of present paper invoke the array plasma antenna can be applied for steering and controlling the strength of Wi-Fi signals as per requirement.

Confirmation of diosmetin 3-O-glucuronide as major metabolite of diosmin in humans, using micro-liquid-chromatography-mass spectrometry and ion mobility mass spectrometry.

PubMed

Silvestro, Luigi; Tarcomnicu, Isabela; Dulea, Constanta; Attili, Nageswara Rao B N; Ciuca, Valentin; Peru, Dan; Rizea Savu, Simona

2013-10-01

Diosmin is a flavonoid often administered in the treatment of chronic venous insufficiency, hemorrhoids, and related affections. Diosmin is rapidly hydrolized in the intestine to its aglicone, diosmetin, which is further metabolized to conjugates. In this study, the development and validations of three new methods for the determination of diosmetin, free and after enzymatic deconjugation, and of its potential glucuronide metabolites, diosmetin-3-O-glucuronide, diosmetin-7-O-glucuronide, and diosmetin-3,7-O-glucuronide from human plasma and urine are presented. First, the quantification of diosmetin, free and after deconjugation, was carried out by high-performance liquid chromatography coupled with tandem mass spectrometry, on an Ascentis RP-Amide column (150 × 2.1 mm, 5 μm), in reversed-phase conditions, after enzymatic digestion. Then glucuronide metabolites from plasma were separated by micro-liquid chromatography coupled with tandem mass spectrometry on a HALO C18 (50 × 0.3 mm, 2.7 μm, 90 Å) column, after solid-phase extraction. Finally, glucuronides from urine were measured using a Discovery HSF5 (100 × 2.1 mm, 5 μm) column, after simple dilution with mobile phase. The methods were validated by assessing linearity, accuracy, precision, low limit of quantification, selectivity, extraction recovery, stability, and matrix effects; results in agreement with regulatory (Food and Drug Administration and European Medicines Agency) guidelines acceptance criteria were obtained in all cases. The methods were applied to a pharmacokinetic study with diosmin (450 mg orally administered tablets). The mean C max of diosmetin in plasma was 6,049.3 ± 5,548.6 pg/mL. A very good correlation between measured diosmetin and glucuronide metabolites concentrations was obtained. Diosmetin-3-O-glucuronide was identified as a major circulating metabolite of diosmetin in plasma and in urine, and this finding was confirmed by supplementary experiments with differential ion-mobility mass spectrometry.

Relevant parameter space and stability of spherical tokamaks with a plasma center column

NASA Astrophysics Data System (ADS)

Lampugnani, L. G.; Garcia-Martinez, P. L.; Farengo, R.

2017-02-01

A spherical tokamak (ST) with a plasma center column (PCC) can be formed inside a simply connected chamber via driven magnetic relaxation. From a practical perspective, the ST-PCC could overcome many difficulties associated with the material center column of the standard ST reactor design. Besides, the ST-PCC concept can be regarded as an advanced helicity injected device that would enable novel experiments on the key physics of magnetic relaxation and reconnection. This is because the concept includes not only a PCC but also a coaxial helicity injector (CHI). This combination implies an improved level of flexibility in the helicity injection scheme required for the formation and sustainment phases. In this work, the parameter space determining the magnetic structure of the ST-PCC equilibria is studied under the assumption of fully relaxed plasmas. In particular, it is shown that the effect of the external bias field of the PCC and the CHI essentially depends on a single parameter that measures the relative amount of flux of these two entities. The effect of plasma elongation on the safety factor profile and the stability to the tilt mode are also analyzed. In the first part of this work, the stability of the system is explained in terms of the minimum energy principle, and relevant stability maps are constructed. While this picture provides an adequate insight into the underlying physics of the instability, it does not include the stabilizing effect of line-tying at the electrodes. In the second part, a dynamical stability analysis of the ST-PCC configurations, including the effect of line-tying, is performed by numerically solving the magnetohydrodynamic equations. A significant stability enhancement is observed when the PCC contains more than the 70% of the total external bias flux, and the elongation is not higher than two.

[Simultaneous determination of tryptophan and its metabolites in plasma by high performance liquid chromatography with on-column derivatization].

PubMed

Feng, Chengya; Gao, Jieying; Zhen, Qianna; Fan, Zimian; Zhu, Mingsong; Yang, Xiangchun; Ding, Min

2013-06-01

A high performance liquid chromatography-ultraviolet/fluorescence detection (HPLC-UV/FLD) with on-column derivatization was established to simultaneously determine tryptophan (Trp), kynurenine (Kyn), 5-hydroxyindole acetic acid (5-Hiaa) and kynurenic acid (Kyna). A Hypersil C-18 column (250 mm x 4.0 mm, 5 microm) was used for the analysis at 30 degrees C. The separation was carried out with the mobile phase consisting of 250 mmol/L zinc acetate (pH 5.5) and acetonitrile (95: 5, v/v) at a flow rate of 0.8 mL/min using 3-nitrotyrosine as internal standard (IS). The excitation (Ex) and emission (Em) wavelengths were set at 278 nm (lambda(ex))/343 nm (lambda(em)) for 5-Hiaa and 244 nm (lambda(ex))/400 nm (lambda(em)) for Kyna, while the wavelengths of ultraviolet detection were set at 360 nm for Kyn and IS, 302 nm for Trp. The recoveries were in the range of 91.62% to 114.17%. The linearities were from 2.50 micromol/L to 320.00 micromol/L for Trp, 0.32 micromol/L to 15.36 micromol/L for Kyn, 3.27 nmol/L to 104.60 nmol/L for 5-Hiaa, and 14.00 nmol/L to 464.80 nmol/L for Kyna. The detection limits were 0.078 micromol/L, 0.056 micromol/L, 0.690 nmol/L and 1.290 nmol/L for Trp, Kyn, 5-Hiaa, and Kyna, respectively. Thirty plasma samples of normal pregnant women and 28 plasma samples of healthy controls were tested, and the results exhibited that the concentrations of Trp, Kyn and Kyna in the plasma of the normal pregnant women were significantly different from those of the control group (all P < 0.01). The method is simple and sensitive with good reproducibility, and it is suitable for clinical measurements.

Validation and application of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous quantification of lopinavir and ritonavir in human plasma using semi-automated 96-well liquid-liquid extraction.

PubMed

Wang, Perry G; Wei, Jack S; Kim, Grace; Chang, Min; El-Shourbagy, Tawakol

2006-10-20

Kaletra is an important antiretroviral drug, which has been developed by Abbott Laboratories. It is composed of lopinavir (low-pin-a-veer) and ritonavir (ri-toe-na-veer). Both have been proved to be human immunodeficiency virus (HIV) protease inhibitors and have substantially reduced the morbidity and mortality associated with HIV-1 infection. We have developed and validated an assay, using liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry (LC/MS/MS), for the routine quantification of lopinavir and ritonavir in human plasma, in which lopinavir and ritonavir can be simultaneously analyzed with high throughput. The sample preparation consisted of liquid-liquid extraction with a mixture of hexane: ethyl acetate (1:1, v/v), using 100 microL of plasma. Chromatographic separation was performed on a Waters Symmetry C(18) column (150 mm x 3.9 mm, particle size 5 microm) with reverse-phase isocratic using mobile phase of 70:30 (v/v) acetonitrile: 2 mM ammonium acetate aqueous solution containing 0.01% formic acid (v/v) at a flow rate of 1.0 mL/min. A Waters symmetry C(18) guard column (20 mm x 3.9 mm, particle size 5 microm) was connected prior to the analytical column, and a guard column back wash was performed to reduce the analytical column contamination using a mixture of tetrahydrofuran (THF), methanol and water (45:45:10, v/v/v). The analytical run was 4 min. The use of a 96-well plate autosampler allowed a batch size up to 73 study samples. A triple-quadrupole mass spectrometer was operated in a positive ion mode and multiple reaction monitoring (MRM) was used for drug quantification. The method was validated over the concentration ranges of 19-5,300 ng/mL for lopinavir and 11-3,100 ng/mL for ritonavir. A-86093 was used as an internal standard (I.S.). The relative standard deviation (RSD) were <6% for both lopinavir and ritonavir. Mean accuracies were between the designed limits (+/-15%). The robust and rapid LC/MS/MS assay has been successfully applied for routine assay to support bioavailability, bioequivalence, and pharmacokinetics studies.

Peculiarities of Efficient Plasma Generation in Air and Water by Short Duration Laser Pulses

NASA Technical Reports Server (NTRS)

Adamovsky, Grigory; Floyd, Bertram M.

2017-01-01

We have conducted experiments to demonstrate an efficient generation of plasma discharges by focused nanosecond pulsed laser beams in air and provided recommendations on the design of optical systems to implement such plasma generation. We have also demonstrated generation of the secondary plasma discharge using the unused energy from the primary one. Focused nanosecond pulsed laser beams have also been utilized to generate plasma in water where we observed self-focusing and filamentation. Furthermore, we applied the laser generated plasma to the decomposition of methylene blue dye diluted in water.

In vivo study on the neurotransmitters and their metabolites change in depressive disorder rat plasma by ultra high performance liquid chromatography coupled to tandem mass spectrometry.

PubMed

Zhao, Longshan; Zheng, Shuning; Su, Guangyue; Lu, Xiumei; Yang, Jingyu; Xiong, Zhili; Wu, Chunfu

2015-04-15

A sensitive and versatile, ultra-high performance, liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method coupled to pre-column derivatization for the simultaneous determination of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), dopamine (DA), norepinephrine (NE), homovanillic acid (HVA), γ-aminobutyric acid (GABA) and glutamic acid (Glu) was developed and validated in rat plasma. The analytes were dansylated under strong alkaline conditions after protein precipitation extraction, which were analyzed on a BEH C18 column using a gradient elution. The lower limit of quantification (LLOQ) values for 5-HT, 5-HIAA, DA, NE, HVA, GABA and Glu were 1.00, 1.00, 0.991, 0.992, 1.02, 1000, and 5030 pmol/mL, respectively. Good linearity was obtained (r > 0.99) and the intra- and inter-day precisions of the method (relative standard deviation, RSD%) were lower than 12%. The method was novel, sensitive and specific which can provide an alternative method for the quantification of neurotransmitters and their metabolites in plasma samples. Copyright © 2015 Elsevier B.V. All rights reserved.

Analysis of 26 amino acids in human plasma by HPLC using AQC as derivatizing agent and its application in metabolic laboratory.

PubMed

Sharma, Gaurav; Attri, Savita Verma; Behra, Bijaylaxmi; Bhisikar, Swapnil; Kumar, Praveen; Tageja, Minni; Sharda, Sheetal; Singhi, Pratibha; Singhi, Sunit

2014-05-01

The present study reports the simultaneous analysis of 26 physiological amino acids in plasma along with total cysteine and homocysteine by high-performance liquid chromatography (HPLC) employing 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) as precolumn derivatizing reagent. Separations were carried out using Lichrospher 100 RP-18e (5 μm) 250 × 4.0 mm column connected to 100 CN 4.0 × 4.0 mm guard column on a quaternary HPLC system and run time was 53 min. Linearity of the peak areas for different concentrations ranging from 2.5 to 100 pmol/μL of individual amino acids was determined. A good linearity (R (2) > 0.998) was achieved in the standard mixture for each amino acid. Recovery of amino acids incorporated at the time of derivatization ranged from 95 to 106 %. Using this method we have established the normative data of amino acids in plasma, the profile being comparable to the range reported in literature and identified cases of classical homocystinuria, cobalamin defect/deficiency, non-ketotic hyperglycinemia, hyperprolinemia, ketotic hyperglycinemia, urea cycle defect and maple syrup urine disease.

Extension of electron cyclotron heating at ASDEX Upgrade with respect to high density operation

NASA Astrophysics Data System (ADS)

Schubert, Martin; Stober, Jörg; Herrmann, Albrecht; Kasparek, Walter; Leuterer, Fritz; Monaco, Francesco; Petzold, Bernhard; Plaum, Burkhard; Vorbrugg, Stefan; Wagner, Dietmar; Zohm, Hartmut

2017-10-01

The ASDEX Upgrade electron cyclotron resonance heating operates at 105 GHz and 140 GHz with flexible launching geometry and polarization. In 2016 four Gyrotrons with 10 sec pulse length and output power close to 1 MW per unit were available. The system is presently being extended to eight similar units in total. High heating power and high plasma density operation will be a part of the future ASDEX Upgrade experiment program. For the electron cyclotron resonance heating, an O-2 mode scheme is proposed, which is compatible with the expected high plasma densities. It may, however, suffer from incomplete single-pass absorption. The situation can be improved significantly by installing holographic mirrors on the inner column, which allow for a second pass of the unabsorbed fraction of the millimetre wave beam. Since the beam path in the plasma is subject to refraction, the beam position on the holographic mirror has to be controlled. Thermocouples built into the mirror surface are used for this purpose. As a protective measure, the tiles of the heat shield on the inner column were modified in order to increase the shielding against unabsorbed millimetre wave power.

An Improved LC-ESI-MS/MS Method to Quantify Pregabalin in Human Plasma and Dry Plasma Spot for Therapeutic Monitoring and Pharmacokinetic Applications.

PubMed

Dwivedi, Jaya; Namdev, Kuldeep K; Chilkoti, Deepak C; Verma, Surajpal; Sharma, Swapnil

2018-06-06

Therapeutic drug monitoring (TDM) of anti-epileptic drugs provides a valid clinical tool in optimization of overall therapy. However, TDM is challenging due to the high biological samples (plasma/blood) storage/shipment costs and the limited availability of laboratories providing TDM services. Sampling in the form of dry plasma spot (DPS) or dry blood spot (DBS) is a suitable alternative to overcome these issues. An improved, simple, rapid, and stability indicating method for quantification of pregabalin in human plasma and DPS has been developed and validated. Analyses were performed on liquid chromatography tandem mass spectrometer under positive ionization mode of electrospray interface. Pregabain-d4 was used as internal standard, and the chromatographic separations were performed on Poroshell 120 EC-C18 column using an isocratic mobile phase flow rate of 1 mL/min. Stability of pregabalin in DPS was evaluated under simulated real-time conditions. Extraction procedures from plasma and DPS samples were compared using statistical tests. The method was validated considering the FDA method validation guideline. The method was linear over the concentration range of 20-16000 ng/mL and 100-10000 ng/mL in plasma and DPS, respectively. DPS samples were found stable for only one week upon storage at room temperature and for at least four weeks at freezing temperature (-20 ± 5 °C). Method was applied for quantification of pregabalin in over 600 samples of a clinical study. Statistical analyses revealed that two extraction procedures in plasma and DPS samples showed statistically insignificant difference and can be used interchangeably without any bias. Proposed method involves simple and rapid steps of sample processing that do not require a pre- or post-column derivatization procedure. The method is suitable for routine pharmacokinetic analysis and therapeutic monitoring of pregabalin.

On the thickness of accretion curtains on magnetized compact objects from analysis of their fast aperiodic time variability.

NASA Astrophysics Data System (ADS)

Semena, Andrey

It is widely accepted that accretion onto magnetized compact objects is channelled to some areas close to magnetic poles of the star. Thickness of this channelled accretion flow intimately depends on details of penetration of highly conducting plasma of the flow to the compact object magnetosphere, i.e. on magnetic diffusivity etc. Until now our knowledge of these plasma properties is scarce. In our work we present our attempts to estimate the thickness of the plasma flow on top of the magnetosphere from observations of accreting intermediate polars (magnetized white dwarfs). We show that properties of aperiodic noise of accreting intermediate polars can be used to put constrains on cooling time of hot plasma, heated in the standing shock wave above the WD surface. Estimates of the cooling time and the mass accretion rate provide us a tool to measure the density of post-shock plasma and the cross-sectional area of the accretion funnel at the WD surface. We have studied aperiodic noise of emission of one of the brightest intermediate polar EX Hya with the help of data in optical and X-ray energy bands. We put an upper limit on the plasma cooling timescale tau <0.2-0.5 sec, on the fractional area of the accretion curtain footprint f < 1.6 × 10(-4) . We show that measurements of accretion column footprints, combined with results of the eclipse mapping, can be used to obtain an upper limit on the penetration depth of the accretion disc plasma at the boundary of the magnetosphere, Delta r / r ≈ 10(-3) If the magnetospheres of accreting neutron stars have similar plasma penetration depths at their boundaries, we predict that footprints of their accretion columns should be very small, with fractional areas < 10(-6) .

Magnetoelectric confinement and stabilization of Z pinch in a soft-x-ray Ar(+8) laser.

PubMed

Szasz, J; Kiss, M; Santa, I; Szatmari, S; Kukhlevsky, S V

2013-05-03

Magnetoelectric confinement and stabilization of the plasma column in a soft-x-ray Ar(+8) laser, which is excited by a capillary Z pinch, via the combined magnetic and electric fields of the gliding surface discharge is experimentally demonstrated. Unlike soft-x-ray lasers excited by the conventional capillary Z pinches, the magnetoelectric confinement and stabilization of plasma do provide the laser operation without using any external preionization circuit.

Measurements of the canonical helicity evolution of a gyrating kinked plasma column

NASA Astrophysics Data System (ADS)

von der Linden, Jens; Sears, Jason; Intrator, Thomas; You, Setthivoine

2017-10-01

Conversions between kinetic and magnetic energy occur over a wide range of plasma scales as exhibited in astrophysical and solar dynamos, and reconnection in the solar corona and laboratory experiments. Canonical flux tubes present the distinct advantage of reconciling all plasma regimes - e.g. kinetic, two-fluid, and MHD - with the topological concept of helicity: twists, writhes, and linkages. This poster presents the first visualization and analysis of the 3D dynamics of canonical flux tubes and their relative helicity evolution from experimental measurements. Ion and electron canonical flux tubes are visualized from Mach, triple, and Ḃ probe measurements at over 10,000 spatial locations of a gyrating kinked plasma column. The flux tubes co-gyrate with the peak density and electron temperature in and out of a measurement volume. The electron and ion canonical flux tubes twist with opposite handedness and the ion flux tube writhes around the electron flux tube. The relative cross helicity between the magnetic and ion flow vorticity flux tubes dominates the relative ion canonical helicity and is anticorrelated with the relative magnetic helicity. The 3D nature of the kink and a reverse eddy current affect the helicity evolution. This work is supported by DOE Grant DE-SC0010340 and the DOE Office of Science Graduate Student Research Program and prepared in part by LLNL under Contract DE-AC52-07NA27344. LLNL-ABS-734669.

Ion flow measurements during the rotating kink behavior of the central column in the HIST device

NASA Astrophysics Data System (ADS)

Yamada, S.; Yoshikawa, T.; Hashimoto, S.; Nishioka, T.; Kikuchi, Y.; Fukumoto, N.; Nagata, M.

2007-11-01

Plasma flow is essentially driven in self-organization and magnetic reconnection process of compact spherical torus (ST) and spheromak in the helicity-driven systems. For example, when reversing the external toroidal field of ST, the direction not only of the plasma current but also of the toroidal ion flow is self-reversed during the formation of the flipped ST relaxed states. Mach probe measurement shows that the velocity of the ion flow reversed after the flip increases to about 20 km/s. We have been newly developing an ion Doppler spectrometer (IDS) system using a compact 16 or 64 channel photomultiplier tube (PMT) in order to measure the spatial profile of ion temperature and rotation velocity in the HIST device. The IDS system consists of a light collection system including optical fibers, 1 m-spectrometer and the PMT detector. The optical fibers covered with glass tubes are inserted into the plasma. The glass tubes can be rotated in the poloidal and the toroidal directions. The new IDS system will be applied to observations of ion temperature and plasma rotation in the flipped ST formation and in the MHD control of kinking behaviors of the central column by using the rotating magnetic field (RMF). Preliminary IDS results will be compared to those from Mach probe measurements in space.

Development and validation of LC-MS/MS methods for the determination of mirabegron and its metabolites in human plasma and their application to a clinical pharmacokinetic study.

PubMed

Teijlingen, Raymond van; Meijer, John; Takusagawa, Shin; Gelderen, Marcel van; Beld, Cas van den; Usui, Takashi

2012-03-01

Mirabegron is being developed for the treatment of overactive bladder. To support the development of mirabegron, including pharmacokinetic studies, liquid chromatography/tandem mass spectrometry methods for mirabegron and eight metabolites (M5, M8, M11-M16) were developed and validated for heparinized human plasma containing sodium fluoride. Four separate bioanalytical methods were developed for the analysis of: (1) mirabegron; (2) M5 and M16; (3) M8; and (4) M11-M15. Either solid-phase extraction or liquid-liquid extraction was used to extract the analytes of interest from matrix constituents. For mirabegron, an Inertsil C₈-3 analytical column was used and detection was performed using a triple-quad mass spectrometer equipped with an atmospheric pressure chemical ionization interface. For the metabolite assays, chromatographic separation was performed through a Phenomenex Synergi Fusion-RP C₁₈ analytical column and detection was performed using a triple-quad mass spectrometer equipped with a Heated Electrospray Ionization interface. The validation results demonstrated that the developed liquid chromatography/tandem mass spectrometry methods were precise, accurate, and selective for the determination of mirabegron and its metabolites in human plasma. All methods were successfully applied in evaluating the pharmacokinetic parameters of mirabegron and metabolites in human plasma. Copyright © 2012 Elsevier B.V. All rights reserved.

Fully automated method for the liquid chromatographic determination of cyproterone acetate in plasma using restricted access material for sample pre-treatment.

PubMed

Christiaens, B; Chiap, P; Rbeida, O; Cello, D; Crommen, J; Hubert, Ph

2003-09-25

A new fully automated method for the quantitative analysis of an antiandrogenic substance, cyproterone acetate (CPA), in plasma samples has been developed using on-line solid-phase extraction (SPE) prior to the determination by reversed-phase liquid chromatography (LC). The automated method was based on the use of a precolumn packed with an internal-surface reversed-phase packing material (LiChrospher RP-4 ADS) for sample clean-up coupled to LC analysis on an octadecyl stationary phase using a column-switching system. A 200-microL volume of plasma sample was injected directly on the precolumn packed with restricted access material using a mixture of water-acetonitrile (90:10, v/v) as washing liquid. The analyte was then eluted in the back-flush mode with the LC mobile phase which consisted of a mixture of phosphate buffer, pH 7.0-acetonitrile (54:46, v/v). The elution profiles of CPA and blank plasma samples on the precolumn and the time needed for analyte transfer from the precolumn to the analytical column were determined. Different compositions of washing liquid and mobile phase were tested to reduce the interference of plasma endogenous components. UV detection was achieved at 280 nm. Finally, the developed method was validated using a new approach, namely the application of the accuracy profile based on the interval confidence at 90% of the total measurement error (bias+standard deviation). The limit of quantification of cyproterone acetate in plasma was determined at 15 ng mL(-1). The validated method should be applicable to the determination of CPA in patients treated by at least 50 mg day(-1).

The X-ray footprint of the circumnuclear disc

NASA Astrophysics Data System (ADS)

Mossoux, Enmanuelle; Eckart, Andreas

2018-03-01

We studied the central regions of the Galactic Centre to determine if the circumnuclear disc (CND) acts as an absorber or a barrier for the central X-rays diffuse emission. After reprocessing 4.6 Ms of Chandra observations, we were able to detect, for the first time, a depression in the X-ray luminosity of the diffuse emission whose size and location correspond to those of the CND. We extracted the X-ray spectra for various regions inside the CND footprint as well as for the region where the footprint is observed and for a region located outside the footprint. We simultaneously fitted these spectra as an optically thin plasma whose absorption by the interstellar medium (ISM) and by the local plasma were fitted independently using the Markov chain Monte Carlo method. The hydrogen column density of the ISM is 7.5 × 1022 cm-2. The X-ray diffuse emission inside the CND footprint is formed by a 2T plasma of 1 and 4 keV with slightly super-solar abundances except for the iron and carbon that are sub-solar. The plasma from the CND, in turn, is better described by a 1T model with abundances and local hydrogen column density that are very different from those of the innermost regions. The large iron abundance in this region confirms that the CND is dominated by the shock-heated ejecta of the Sgr A East supernova remnant. We deduced that the CND rather acts as a barrier for the Galactic Centre plasma and that the plasma located outside the CND may correspond to the collimated outflow possibly created by Sgr A* or the interaction between the wind of massive stars and the mini-spiral material.

Liquid chromatography-electrospray mass spectrometry determination of ibogaine and noribogaine in human plasma and whole blood. Application to a poisoning involving Tabernanthe iboga root.

PubMed

Kontrimaviciūte, Violeta; Breton, Hélène; Mathieu, Olivier; Mathieu-Daudé, Jean-Claude; Bressolle, Françoise M M

2006-11-07

A liquid chromatography/electrospray ionization mass spectrometry (LC-ESI-MS) method was developed for the first time for the determination of ibogaine and noribogaine in human plasma and whole blood. The method involved solid phase extraction of the compounds and the internal standard (fluorescein) from the two matrices using OasisHLB columns. LC separation was performed on a Zorbax eclipse XD8 C8 column (5 microm) with a mobile phase of acetonitrile containing 0.02% (v/v) trimethylamine and 2mM ammonium formate buffer. MS data were acquired in single ion monitoring mode at m/z 311.2, 297.2 and 332.5 for ibogaine, noribogaine and fluorescein, respectively. The drug/internal standard peak area ratios were linked via a quadratic relationship to plasma (0.89-179 microg/l for ibogaine; 1-200 microg/l for noribogaine) and to whole blood concentrations (1.78-358 microg/kg for ibogaine; 2-400 microg/kg for noribogaine). Precision ranged from 4.5 to 13% and accuracy was 89-102%. Dilution of the samples had no influence on the performance of the method. Extraction recoveries were > or =94% in plasma and > or =57% in whole blood. The lower limits of quantitation were 0.89 microg/l for ibogaine and 1 microg/l for noribogaine in plasma, and 1.78 microg/kg for ibogaine and 2 microg/kg for noribogaine in whole blood. In frozen plasma samples, the two drugs were stable for at least 1 year. In blood, ibogaine and noribogaine were stable for 4h at 4 degrees C and 20 degrees C and 2 months at -20 degrees C. The method was successfully used for the analysis of a poisoning involving Tabernanthe iboga root.

Neutron angular distribution in a plasma focus obtained using nuclear track detectors.

PubMed

Castillo-Mejía, F; Herrera, J J E; Rangel, J; Golzarri, J I; Espinosa, G

2002-01-01

The dense plasma focus (DPF) is a coaxial plasma gun in which a high-density, high-temperature plasma is obtained in a focused column for a few nanoseconds. When the filling gas is deuterium, neutrons can be obtained from fusion reactions. These are partially due to a beam of deuterons which are accelerated against the background hot plasma by large electric fields originating from plasma instabilities. Due to a beam-target effect, the angular distribution of the neutron emission is anisotropic, peaked in the forward direction along the axis of the gun. The purpose of this work is to illustrate the use of CR-39 nuclear track detectors as a diagnostic tool in the determination of the time-integrated neutron angular distribution. For the case studied in this work, neutron emission is found to have a 70% contribution from isotropic radiation and a 30% contribution from anisotropic radiation.

Electromagnetic resonances of plasma column between two metallic plates

NASA Astrophysics Data System (ADS)

Dvinin, Sergey; Dovzhenko, Vitaly; Sinkevich, Oleg

2015-09-01

It is known that there are two types of electrodynamic resonances of bounded supercritical plasma, placed between the two metal planes are possible. The first type is associated with the excitation of surface waves propagating along the lateral surface. The second one is caused by standing surface waves in the sheath at plasma-metal boundary. This work is concerned with theoretical study of the resonance properties of plasma slab in cases where both effects can be observed together. Resonance densities and frequencies are calculated. Solution of Maxwell's equations is demonstrated that directions of energy flows in first and second cases are opposite. Energy transfer to lateral surface waves is prevailing, if the field frequency is higher than the frequency, corresponding to the geometric plasma-sheath resonance. Amplitude of waves at plasma metal boundary becomes greater in opposite case. Discharge properties in both cases are calculated including joint excitation.

Autoresonant Control of Elliptical Non-neutral Plasmas

NASA Astrophysics Data System (ADS)

Friedland, Lazar

1999-11-01

It is shown that placing a magnetized non-neutral plasma column in a weak oscillating transverse quadrupolar potential with chirped oscillation frequency allows excitation and control of the ellipticity and rotation phase of the plasma cross section. For a given chirp rate of the driving frequency, the phenomenon has a sharp threshold on the amplitude of the perturbing potential. The effect is analogous to that reported in controlling Kirchhoff vortices in fluid dynamics [1]. The ellipticity of the plasma cross section is manipulated by using autoresonance (nonlinear phase locking) in the system between the ExB drifting plasma particles and adiabatically varying driving potential. A similar idea was used recently in controlling the l=1 diocotron mode in a non-neutral plasma [2]. [1] L. Friedland, Phys. Rev. E59, 4106 (1999). [2] J. Fajans, E. Gilson, and L. Friedland, Phys. Rev. Lett. 82, 4444 (1999).

Maximum Potential Hydrogen Gas Retention in the sRF Resin Ion Exchange Column for the LAWPS Process

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gauglitz, Phillip A.; Wells, Beric E.; Bottenus, Courtney LH

The Low-Activity Waste Pretreatment System (LAWPS) is being developed to provide treated supernatant liquid from the Hanford tank farms directly to the Low-Activity Waste (LAW) Vitrification Facility at the Hanford Tank Waste Treatment and Immobilization Plant. The design and development of the LAWPS is being conducted by Washington River Protection Solutions, LLC. A key process in LAWPS is the removal of radioactive Cs in ion exchange (IX) columns filled with spherical resorcinol-formaldehyde (sRF) resin. One accident scenario being evaluated is the loss of liquid flow through the sRF resin bed after it has been loaded with radioactive Cs and hydrogenmore » gas is being generated by radiolysis. In normal operations, the generated hydrogen is expected to remain dissolved in the liquid and be continuously removed by liquid flow. For an accident scenario with a loss of flow, hydrogen gas can be retained within the IX column both in the sRF resin and below the bottom screen that supports the resin within the column. The purpose of this report is to summarize calculations that estimate the upper-bound volume of hydrogen gas that can be retained in the column and potentially be released to the headspace of the IX column or to process equipment connected to the IX column and, thus, pose a flammability hazard.« less

A new LC-MS/MS bioanalytical method for atenolol in human plasma and milk.

PubMed

Phyo Lwin, Ei Mon; Gerber, Cobus; Song, Yunmei; Leggett, Catherine; Ritchie, Usha; Turner, Sean; Garg, Sanjay

2017-04-01

A new sensitive LC-MS/MS method for the quantification of atenolol in human plasma and milk has been developed for clinical lactation studies. Atenolol and the internal standard, phenazone, were extracted from biological matrices by protein precipitation. A Phenomenex ® C-18 column and gradient chromatographic conditions were used for separation of the analyte, followed by detection with MS. Stability of samples was confirmed for atenolol in human plasma and milk for up to 3 months. Linearity range of 1-800 ng/ml (r 2 = 0.9995), the precision within 15% CV and the recovery of the analyte (80-100% range) were achieved. A new validated analytical method for atenolol in plasma and milk was developed.

Fast discharge in a plasma gun with hemispherical insulator

NASA Astrophysics Data System (ADS)

Antsiferov, P. S.; Dorokhin, L. A.; Sidelnikov, Yu. V.; Koshelev, K. N.

2009-05-01

A method of creation of hot dense plasma is proposed. It is based on cumulation of a shockwave, which originates on a hemispherical surface of insulator of plasma gun. The results of first experiments are presented. The shock wave is driven by fast electrical discharge (dI /dt>1012 A/s). The inductive storage with semiconductor opening switch is used as a current driver. Time resolved pin-hole images and vacuum ultraviolet (vuv) spectra are studied. Shockwaves from hemispherical insulator with 4 mm radius create plasma with a form of column about 1 mm diameter and 3-4 mm length. vuv spectra contain the lines of Ar ions that corresponds to the electron temperature about 20 eV. Possible practical application is discussed.

HPLC–electrospray mass spectrometric assay for the determination of (R,R)-fenoterol in rat plasma

PubMed Central

Siluk, Danuta; Kim, Hee Seung; Cole, Tyler; Wainer, Irving W.

2008-01-01

A fast and specific liquid chromatography–mass spectrometry method for the determination of (R,R)-fenoterol ((R,R)-Fen) in rat plasma has been developed and validated. (R,R)-Fen was extracted from 125 µl of plasma using solid phase extraction and analyzed on Atlantis HILIC Silica 3 µm column. The mobile phase was composed of acetonitrile:ammonium acetate (pH 4.1; 20 mM) (85:15, v/v), at a flow rate of 0.2 ml/min. The lower limit of detection (LLOD) was 2 ng/ml . The procedure was validated and applied to the analysis of plasma samples from rats previously administered (R,R)-Fen in an intravenous bolus. PMID:18617349

Optimization of a liquid chromatographic method for determination of malachite green and its metabolites in fish tissues

USGS Publications Warehouse

Plakas, S.M.; ELSaid, K.R.; Stehly, G.R.; Roybal, J.E.

1995-01-01

A liquid chromatographic (LC) method was adapted and optimized for the determination of malachite green and its metabolites in fish plasma and muscle, Residues in plasma were extracted with acetonitrile, the extract was evaporated to dryness, and residues were resolubilized for LC analysis, Residues in muscle were extracted with an acetonitrile-acetate buffer mixture, reextracted with acetonitrile, and partitioned into methylene chloride with final cleanup on alumina and propylsulfonic acid solid-phase extraction columns, Residue levels were determined by using an LC cyano column with a PbO2 postcolumn and visible detection (618 nm). Overall mean recoveries of parent malachite green (MG-C) and its major metabolite, leucomalachite green (MG-L), from plasma were 93 and 87%, respectively, at fortification levels ranging from 25 to 250 ppb, Overall mean recoveries of MG-C and MG-L from muscle were 85 and 95%, respectively, at fortification levels ranging from 5 to 100 ppb, Relative standard deviations (RSDs) of recoveries at all fortification levels ranged from 3.9 to 7.0% for plasma and from 2.1 to 5.2% for muscle, The method was applied to incurred residues in tissues sampled from catfish after waterborne exposure to [C-14]MG-C. Mean recoveries of total radioactive residues in plasma and muscle throughout the extraction and cleanup process were 88 and 87%, respectively, and corresponding RSDs for MG-C and MG-L were in the same range as those for fortified tissues, MG-L, was confirmed as the major metabolite of MG-C in catfish.

Simultaneous determination of loxoprofen and its diastereomeric alcohol metabolites in human plasma and urine by a simple HPLC-UV detection method.

PubMed

Choo, K S; Kim, I W; Jung, J K; Suh, Y G; Chung, S J; Lee, M H; Shim, C K

2001-06-01

A simple, reliable HPLC-UV detection method was developed for the simultaneous determination of loxoprofen and its metabolites (i.e. trans- and cis-alcohol metabolites), in human plasma and urine samples. The method involves the addition of a ketoprofen (internal standard) solution in methanol, zinc sulfate solution and acetonitrile to plasma and urine samples, followed by centrifugation. An aliquot of the supernatant was evaporated to dryness, and the residue reconstituted in a mobile phase (acetonitrile:water=35:65 v/v, pH 3.0). An aliquot of the solution was then directly injected into the HPLC system. Separations were performed on octadecylsilica column (250x4.5 mm, 5 microm) with a guard column (3.2x1.5 cm, 7 microm) at ambient temperature. Loxoprofen and the metabolites in the eluent were monitored at 220 nm (a.u.f.s. 0.005). Coefficients of variations (CV%) and recoveries for loxoprofen and its metabolites were below 10 and over 96%, respectively, in the 200 approximately 15000 ng ml(-1) range for plasma and 500 approximately 50000 ng ml(-1) range for urine. Calibration curves for all the compounds in the plasma and urine were linear over the above-mentioned concentration ranges with a common correlation coefficient of 0.999. The detection limit of the present method was 100 ng for all the compounds. These results indicate that the present method is very simple and readily applicable to routine bioavailability studies of these compounds with an acceptable sensitivity.

Protocols for the measurement of the F2-isoprostane, 15(S)-8-iso-prostaglandin F2α, in biological samples by GC-MS or GC-MS/MS coupled with immunoaffinity column chromatography.

PubMed

Tsikas, Dimitrios; Suchy, Maria-Theresia

2016-04-15

Arachidonic acid, the origin of the eicosanoids family, occurs in biological samples as free acid and as ester in lipids. Free arachidonic acid is oxidized to numerous metabolites by means of enzymes including cyclooxygenase (COX). Arachidonic acid esterified to lipids is attacked by reactive oxygen species (ROS) to generate numerous oxidized arachidonic acid derivatives. Generally, it is assumed that ROS-derived arachidonic acid derivatives are distinct from those generated by enzymes such as COX. Therefore, ROS-generated eicosanoids are considered specific biomarkers of oxidative stress. However, there are serious doubts concerning a strict distinction between the enzyme-derived eicosanoids and the ROS-derived iso-eicosanoids. Prominent examples are prostaglandin F2α (PGF2α) and 15(S)-8-iso-prostaglandin F2α (15(S)-8-iso-PGF2α) which have been originally considered to exclusively derive from COX and ROS, respectively. There is convincing evidence that both COX and ROS can oxidize arachidonic acid to PGF2α and 15(S)-8-iso-PGF2α. Thus, many results previously reported for 15(S)-8-iso-PGF2α as exclusive ROS-dependent reaction product, and consequently as a specific biomarker of oxidative stress, require a careful re-examination which should also consider the analytical methods used to measure 15(S)-8-iso-PGF2α. This prominent but certainly not the only example underlines more than ever the importance of the analytical chemistry in basic and clinical research areas of oxidative stress. In the present work, we report analytical protocols for the reliable quantitative determination of 15(S)-8-iso-PGF2α in human biological samples including plasma and urine by mass spectrometry coupled to gas chromatography (GC-MS, GC-MS/MS) after specific isolation of endogenous 15(S)-8-iso-PGF2α and the externally added internal standard [3,3',4,4'-(2)H4]-15(S)-8-iso-PGF2α by immunoaffinity column chromatography (IAC). 15(S)-8-iso-PGF2α esterified to plasma lipids is hydrolysed by KOH. 15(S)-8-iso-PGF2α and [3,3',4,4'-(2)H4]-15(S)-8-iso-PGF2α are analyzed as pentafluorobenzyl ester trimethylsilyl ether derivatives in the electron-capture negative-ion chemical ionization mode. Copyright © 2014 Elsevier B.V. All rights reserved.

Computational study of sheath structure in oxygen containing plasmas at medium pressures

NASA Astrophysics Data System (ADS)

Hrach, Rudolf; Novak, Stanislav; Ibehej, Tomas; Hrachova, Vera

2016-09-01

Plasma mixtures containing active species are used in many plasma-assisted material treatment technologies. The analysis of such systems is rather difficult, as both physical and chemical processes affect plasma properties. A combination of experimental and computational approaches is the best suited, especially at higher pressures and/or in chemically active plasmas. The first part of our study of argon-oxygen mixtures was based on experimental results obtained in the positive column of DC glow discharge. The plasma was analysed by the macroscopic kinetic approach which is based on the set of chemical reactions in the discharge. The result of this model is a time evolution of the number densities of each species. In the second part of contribution the detailed analysis of processes taking place during the interaction of oxygen containing plasma with immersed substrates was performed, the results of the first model being the input parameters. The used method was the particle simulation technique applied to multicomponent plasma. The sheath structure and fluxes of charged particles to substrates were analysed in the dependence on plasma pressure, plasma composition and surface geometry.

Quasi-adiabatic vacuum-based column housing for very high-pressure liquid chromatography.

PubMed

Gritti, Fabrice; Gilar, Martin; Jarrell, Joseph A

2016-07-22

A prototype vacuum-based (10(-6)Torr) column housing was built to thermally isolate the chromatographic column from the external air environment. The heat transfer mechanism is solely controlled by surface radiation, which was minimized by wrapping the column with low-emissivity aluminum tape. The adiabaticity of the column housing was quantitatively assessed from the measurement of the operational pressure and fluid temperature at the outlet of a 2.1mm×100mm column (sub-2 μm particles). The pressure drop along the column was raised up to 1kbar. The enthalpy balance of the eluent (water, acetonitrile, and one water/acetonitrile mixture, 70/30, v/v) showed that less than 1% of the viscous heat generated by friction of the fluid against the packed bed was lost to the external air environment. Such a vacuum-based column oven minimizes the amplitude of the radial temperature gradients across the column diameter and maximizes its resolving power. Copyright © 2016 Elsevier B.V. All rights reserved.

Drift-Alfven eigenmodes in inhomogeneous plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vranjes, J.; Poedts, S.

2006-03-15

A set of three nonlinear equations describing drift-Alfven waves in a nonuniform magnetized plasma is derived and discussed both in linear and nonlinear limits. In the case of a cylindric radially bounded plasma with a Gaussian density distribution in the radial direction the linearized equations are solved exactly yielding general solutions for modes with quantized frequencies and with radially dependent amplitudes. The full set of nonlinear equations is also solved yielding particular solutions in the form of rotating radially limited structures. The results should be applicable to the description of electromagnetic perturbations in solar magnetic structures and in astrophysical column-likemore » objects including cosmic tornados.« less

Sensitive analysis of blonanserin, a novel antipsychotic agent, in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry.

PubMed

Ogawa, Tadashi; Hattori, Hideki; Kaneko, Rina; Ito, Kenjiro; Iwai, Masayo; Mizutani, Yoko; Arinobu, Tetsuya; Ishii, Akira; Suzuki, Osamu; Seno, Hiroshi

2010-01-01

A rapid and sensitive method for analysis of blonanserin in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry is presented. After pretreatment of a plasma sample by solid-phase extraction, blonanserin was analyzed by the system with a C(18) column. This method gave satisfactory recovery rates, reproducibility, and good linearity of calibration curve in the range of 0.01-10.0 ng/mL for quality control samples spiked with blonanserin. The detection limit was as low as 1 pg/mL. This method seems very useful in forensic and clinical toxicology and pharmacokinetic studies.

Scattering of Microwaves by Steady-State Plasma Slabs, Columns, and Layers at Atmospheric Pressure

DTIC Science & Technology

1998-03-01

permeability unity is- (Fig 0) SÖ$ftS?S5 Pressure Plasmas Y=J7(er) . 2071 (1) where y is the complex propagation coefficient, w is the wave...a phase dependence expjtot-Yxl to a i„ ., permeability nnTty,^ J ’°SSy med’Um °f reIat<- ■j^r)^ • (1) where y is the complex propagation...preservation is an. issue. Some examples are food (solid or liquid) sterilization, pharmaceutical applications, and environmental applications ( soil

Two mechanisms of resonance overlapping in excitation of azimuthal surface waves by rotating relativistic electron beams

NASA Astrophysics Data System (ADS)

Girka, Igor O.; Pavlenko, Ivan V.; Thumm, Manfred

2018-05-01

Azimuthal surface waves are electromagnetic eigenwaves of cylindrical plasma-filled metallic waveguides with a stationary axial magnetic field. These waves with extraordinary polarization can effectively interact with relativistic electron beams rotating along large Larmor orbits in the gap, which separates the plasma column from the waveguide wall. Both widening the layer and increasing the beam particle density are demonstrated to cause resonance overlapping seen from the perspective of the growth rate dependence on the effective wave number.

Application of antibody-mediated extraction for the stereoselective determination of the active metabolite of loxoprofen in human and rat plasma.

PubMed

Takasaki, W; Tanaka, Y

1992-01-01

Antibody-mediated extraction followed by chiral high-performance liquid chromatography (HPLC) was applied to stereoselective determination in human and rat plasma of the active metabolite [(2S,1'R,2'S)-trans-alcohol] with three chiral centers of Loxoprofen, a 2-arylpropionic acid antiinflammatory agent after oral administration. Antiserum against the (1'R,2'S)-cyclopentanol moiety was obtained from a rabbit immunized with bovine serum albumin conjugate linked to the propionic acid moiety, in which another chiral center is located. Then, the immunoglobulin G purified by a protein A column was coupled to BrCN-activated Sepharose 4B. Plasma samples were applied to the immobilized antibody column. After washing the column to remove unrequired stereoisomers, a mixture of two diastereomers whose configurations were 1'R,2'S in the cyclopentanol moiety was extracted with 95% methanol. The solvent was evaporated and the residue was derivatized with (+)-(R)-1-(1-naphthyl)ethylamine as a chiral reagent to separate the diastereomers by HPLC. This combined analytical method showed the stereoselective metabolism of Loxoprofen in human, that is, 64% of the total amount of four trans-alcohol stereoisomers was in the 2S,1'R,2'S form, which is the active metabolite. This phenomenon was also observed in rats given Loxoprofen and its (2S)- and (2R)-isomers, and is explained by stereoselective ketone reduction of Loxoprofen to the (1'R,2'S)-trans-alcohol and inversion from 2R to 2S in the propionic acid moiety. Antibody-mediated extraction should be a selective and simple clean-up method for determining haptens with complicated structures, combined with an appropriate analytical method.

Evidence for Particle Inward Transport, Theoretical prediction and Importance for Reacting Plasmas

NASA Astrophysics Data System (ADS)

Sharky, N.; Coppi, B.; Mazzotta, C.

2017-10-01

The fact that particle transport cannot be described by a diffusion equation but by one that would include an inflow term, involving transport in the direction of the density gradient, was evidenced by experiments on magnetically confined plasmas in which the central plasma density was observed to increase as a result of gas injection at the edge of the plasma column. The validity of the proposed equation has been repeatedly confirmed over the years and limitations for the occurrence of particle inflow in a variety of experimental conditions have been uncovered. The direct experimental observation of the inward propagating particle cloud leading to a profile peaking is described and the effects of different degrees of density peaking in fusion burning plasmas are analyzed. Sponsored in part by the U.S. DoE.

Linear theory of plasma Čerenkov masers

NASA Astrophysics Data System (ADS)

Birau, M.

1996-11-01

A different theoretical model of Čerenkov instability in the linear amplification regime of plasma Čerenkov masers is developed. The model assumes a cold relativistic annular electron beam propagating through a column of cold dense plasma, the two bodies being immersed in an infinite magnetic guiding field inside a perfect cylindrical waveguide. In order to simplify the calculations, a radial rectangular distribution of plasma and beam density is assumed and only azimuthal symmetric modes are under investigation. The model's difference consists of taking into account the whole plasma and beam electromagnetic structures in the interpretation of the Čerenkov instability. This model leads to alternative results such as the possibility of emission at several frequencies. In addition, the electric field is calculated taking into account its radial phase dependence, so that a map of the field in the interaction region can be presented.

Magnetic field generation in core-sheath jets via the kinetic Kelvin-Helmholtz instability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nishikawa, K.-I.; Hardee, P. E.; Duţan, I.

2014-09-20

We have investigated magnetic field generation in velocity shears via the kinetic Kelvin-Helmholtz instability (kKHI) using a relativistic plasma jet core and stationary plasma sheath. Our three-dimensional particle-in-cell simulations consider plasma jet cores with Lorentz factors of 1.5, 5, and 15 for both electron-proton and electron-positron plasmas. For electron-proton plasmas, we find generation of strong large-scale DC currents and magnetic fields that extend over the entire shear surface and reach thicknesses of a few tens of electron skin depths. For electron-positron plasmas, we find generation of alternating currents and magnetic fields. Jet and sheath plasmas are accelerated across the shearmore » surface in the strong magnetic fields generated by the kKHI. The mixing of jet and sheath plasmas generates a transverse structure similar to that produced by the Weibel instability.« less

Dynamic and spectroscopic characteristics of atmospheric gliding arc in gas-liquid two-phase flow

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tu, X.; Yu, L.; Yan, J. H.

In this study, an atmospheric alternating-current gliding arc device in gas-liquid two-phase flow has been developed for the purpose of waste water degradation. The dynamic behavior of the gas-liquid gliding arc is investigated through the oscillations of electrical signals, while the spatial evolution of the arc column is analyzed by high speed photography. Different arc breakdown regimes are reported, and the restrike mode is identified as the typical fluctuation characteristic of the hybrid gliding arc in air-water mixture. Optical emission spectroscopy is employed to investigate the active species generated in the gas-liquid plasma. The axial evolution of the OH (309more » nm) intensity is determined, while the rotational and vibrational temperatures of the OH are obtained by a comparison between the experimental and simulated spectra. The significant discrepancy between the rotational and translational temperatures has also been discussed.« less

Lightning-produced Carbon Species in the Atmosphere of Saturn

NASA Astrophysics Data System (ADS)

Delitsky, Mona; Baines, K. H.

2010-10-01

Recent studies by Baines et al (2009) indicate that thunderstorm-associated clouds on Saturn are spectrally dark from 0.7 to 4 um, darker than regular clouds. This darkening is found to be consistent with the presence of particles of elemental carbon, such as in the form of soot particles mixed in with spectrally bright condensates. This carbon is thought to be generated by lightning-induced dissociation of methane. Lightning on Saturn will input large amounts of energy to a narrow column of atmosphere and generate products at high energies such as radicals and ions. After the column cools down, the new chemical species recombine and are frozen into a new chemical equilibrium. Experimental studies in the literature of reactions of methane and other gases in plasma discharges (which simulate lightning) indicate that, even with high ratios of hydrogen/methane, the elemental carbon obtained will form solid dark particles that persist and have a very high C/H ratio. Basically, they are mostly pure carbon, in the form of soot, amorphous carbon, graphite, graphene, polycyclic aromatic hydrocarbons, carbon black, carbon onions, etc. Hydrogen will act as a sealant onto the particles and attach to dangling bonds on their growing surfaces. Even in experiments to form the most crystalline allotrope of carbon, that is, diamond, the presence of hydrogen does not inhibit diamond formation, even at the low pressures in the atmospheres of the Jovian planets or in the interstellar medium (Allamandola et al 1991). Therefore, some form of elemental carbon is likely produced in Saturnian storm clouds and may occur as dark particles of either amorphous carbon, PAHs or crystalline carbon in a form such as graphite. ..Refs: Baines et al., PSS 57, 1650-1658 (2009) ; Allamandola et al., Meteoritics 26, 313 (1991).

Arc plasma generator of atomic driver for steady-state negative ion source.

PubMed

Ivanov, A A; Belchenko, Yu I; Davydenko, V I; Ivanov, I A; Kolmogorov, V V; Listopad, A A; Mishagin, V V; Putvinsky, S V; Shulzhenko, G I; Smirnov, A

2014-02-01

The paper reviews the results of development of steady-state arc-discharge plasma generator with directly heated LaB6 cathode. This arc-discharge plasma generator produces a plasma jet which is to be converted into an atomic one after recombination on a metallic plate. The plate is electrically biased relative to the plasma in order to control the atom energies. Such an intensive jet of hydrogen atoms can be used in negative ion sources for effective production of negative ions on a cesiated surface of plasma grid. All elements of the plasma generator have an augmented water cooling to operate in long pulse mode or in steady state. The thermo-mechanical stresses and deformations of the most critical elements of the plasma generator were determined by simulations. Magnetic field inside the discharge chamber was optimized to reduce the local power loads. The first tests of the steady-state arc plasma generator prototype have performed in long-pulse mode.

Liquid chromatographic extraction medium

DOEpatents

Horwitz, E. Philip; Dietz, Mark L.

1994-01-01

A method and apparatus for extracting strontium and technetium values from biological, industrial and environmental sample solutions using a chromatographic column is described. An extractant medium for the column is prepared by generating a solution of a diluent containing a Crown ether and dispersing the solution on a resin substrate material. The sample solution is highly acidic and is introduced directed to the chromatographic column and strontium or technetium is eluted using deionized water.

Volatile Transport by Volcanic Plumes on Earth, Venus and Mars

NASA Technical Reports Server (NTRS)

Glaze, Lori S.; Self, Stephen; Baloga, Steve; Stofan, Ellen R.

2012-01-01

Explosive volcanic eruptions can produce sustained, buoyant columns of ash and gas in the atmosphere (Fig. 1). Large flood basalt eruptions may also include significant explosive phases that generate eruption columns. Such eruptions can transport volcanic volatiles to great heights in the atmosphere. Volcanic eruption columns can also redistribute chemical species within the atmosphere by entraining ambient atmosphere at low altitudes and releasing those species at much higher altitudes.

Transferring research data to producers

USDA-ARS?s Scientific Manuscript database

A column will be written for Cow Country News, a monthly newspaper published by the Kentucky Cattlemen Association. The column will present research findings and technologies generated by collaborative research between the USDA-ARS Forage-Animal Production Research Unit and the University of Kentuc...

Apparatus and process for the separation of hydrophobic and hydrophilic particles using microbubble column flotation together with a process and apparatus for generation of microbubbles

DOEpatents

Yoon, Roe-Hoan; Adel, Gregory T.; Luttrell, Gerald H.

1992-01-01

A method and apparatus are disclosed for the microbubble flotation separation of very fine and coarse particles, especially coal and minerals, so as to produce high purity and high recovery efficiency. This is accomplished through the use of a flotation column, microbubbles, recycling of the flotation pulp, and countercurrent wash water to gently wash the froth. Also disclosed are unique processes and apparatus for generating microbubbles for flotation in a highly efficient and inexpensive manner using either a porous tube or in-line static generators.

Apparatus for the separation of hydrophobic and hydrophilic particles using microbubble column flotation together with a process and apparatus for generation of microbubbles

DOEpatents

Yoon, Roe-Hoan; Adel, Gregory T.; Luttrell, Gerald H.

1995-01-01

An apparatus is disclosed for the microbubble flotation separation of very fine and coarse particles, especially coal, and minerals so as to produce high purity and high recovery efficiency. This is accomplished through the use of a flotation column, microbubbles, recycling of the flotation pulp, and countercurrent wash water to gently wash the froth. Also disclosed are unique processes and apparatus for generating microbubbles for flotation in a highly efficient and inexpensive manner using either a porous tube or in-line static generators.

Apparatus for the separation of hydrophobic and hydrophilic particles using microbubble column flotation together with a process and apparatus for generation of microbubbles

DOEpatents

Yoon, R.H.; Adel, G.T.; Luttrell, G.H.

1995-03-14

An apparatus is disclosed for the microbubble flotation separation of very fine and coarse particles, especially coal, and minerals so as to produce high purity and high recovery efficiency. This is accomplished through the use of a flotation column, microbubbles, recycling of the flotation pulp, and countercurrent wash water to gently wash the froth. Also disclosed are unique processes and apparatus for generating microbubbles for flotation in a highly efficient and inexpensive manner using either a porous tube or in-line static generators. 14 figs.

Apparatus and process for the separation of hydrophobic and hydrophilic particles using microbubble column flotation together with a process and apparatus for generation of microbubbles

DOEpatents

Yoon, Roe-Hoan; Adel, Gregory T.; Luttrell, Gerald H.

1998-01-01

A method and apparatus are disclosed for the microbubble flotation separation of very fine and coarse particles, especially coal and minerals, so as to produce high purity and high recovery efficiency. This is accomplished through the use of a flotation column, microbubbles, recycling of the flotation pulp, and countercurrent wash water to gently wash the froth. Also disclosed are unique processes and apparatus for generating microbubbles for flotation in a highly efficient and inexpensive manner using either a porous tube or in-line static generators.

Impact of plasma jet vacuum ultraviolet radiation on reactive oxygen species generation in bio-relevant liquids

NASA Astrophysics Data System (ADS)

Jablonowski, H.; Bussiahn, R.; Hammer, M. U.; Weltmann, K.-D.; von Woedtke, Th.; Reuter, S.

2015-12-01

Plasma medicine utilizes the combined interaction of plasma produced reactive components. These are reactive atoms, molecules, ions, metastable species, and radiation. Here, ultraviolet (UV, 100-400 nm) and, in particular, vacuum ultraviolet (VUV, 10-200 nm) radiation generated by an atmospheric pressure argon plasma jet were investigated regarding plasma emission, absorption in a humidified atmosphere and in solutions relevant for plasma medicine. The energy absorption was obtained for simple solutions like distilled water (dH2O) or ultrapure water and sodium chloride (NaCl) solution as well as for more complex ones, for example, Rosewell Park Memorial Institute (RPMI 1640) cell culture media. As moderate stable reactive oxygen species, hydrogen peroxide (H2O2) was studied. Highly reactive oxygen radicals, namely, superoxide anion (O2•-) and hydroxyl radicals (•OH), were investigated by the use of electron paramagnetic resonance spectroscopy. All species amounts were detected for three different treatment cases: Plasma jet generated VUV and UV radiation, plasma jet generated UV radiation without VUV part, and complete plasma jet including all reactive components additionally to VUV and UV radiation. It was found that a considerable amount of radicals are generated by the plasma generated photoemission. From the experiments, estimation on the low hazard potential of plasma generated VUV radiation is discussed.

Theoretical issues on the spontaneous rotation of axisymmetric plasmas

NASA Astrophysics Data System (ADS)

Coppi, B.; Zhou, T.

2014-09-01

An extensive series of experiments have confirmed that the observed ‘spontaneous rotation’ phenomenon in axisymmetric plasmas is related to the confinement properties of these plasmas and connected to the excitation of collective modes associated with these properties (Coppi 2000 18th IAEA Fusion Energy Conf. (Sorrento, Italy, 2000) THP 1/17, www-pub.iaea.org/MTCD/publications/PDF/csp_008c/html/node343.htm and Coppi 2002 Nucl. Fusion 42 1). In particular, radially localized modes can extract angular momentum from the plasma column from which they grow while the background plasma has to recoil in the direction opposite to that of the mode phase velocity. In the case of the excitation of the plasma modes at the edge, the loss of their angular momentum can be connected to the directed particle ejection to the surrounding medium. The recoil angular momentum is then redistributed inside the plasma column mainly by the combination of an effective viscous diffusion and an inward angular momentum transport velocity that is connected, for instance, to ion temperature gradient (ITG) driven modes. The linear and quasi-linear theories of the collisionless trapped electron modes and of the toroidal ITG driven modes are re-examined in the context of their influence on angular momentum transport. Internal modes that produce magnetic reconnection and are electromagnetic in nature, acquire characteristic phase velocity directions in high temperature regimes and become relevant to the ‘generation’ of angular momentum. The drift-tearing mode, the ‘complex’ reconnecting mode and the m0 = 1 internal mode belong to this category, the last mode acquiring different features depending on the strength of its driving factor. Toroidal velocity profiles that reproduce the experimental observations are obtained considering a global angular momentum balance equation that includes the localized sources associated with the excited internal electrostatic and electromagnetic modes besides the appropriate diffusive and the inward angular momentum transparent terms.

Investigation of the short argon arc with hot anode. II. Analytical model

NASA Astrophysics Data System (ADS)

Khrabry, A.; Kaganovich, I. D.; Nemchinsky, V.; Khodak, A.

2018-01-01

A short atmospheric pressure argon arc is studied numerically and analytically. In a short arc with an inter-electrode gap of several millimeters, non-equilibrium effects in plasma play an important role in operation of the arc. High anode temperature leads to electron emission and intensive radiation from its surface. A complete, self-consistent analytical model of the whole arc comprising of models for near-electrode regions, arc column, and a model of heat transfer in cylindrical electrodes was developed. The model predicts the width of non-equilibrium layers and arc column, voltages and plasma profiles in these regions, and heat and ion fluxes to the electrodes. Parametric studies of the arc have been performed for a range of the arc current densities, inter-electrode gap widths, and gas pressures. The model was validated against experimental data and verified by comparison with numerical solution. Good agreement between the analytical model and simulations and reasonable agreement with experimental data were obtained.

Investigation of the short argon arc with hot anode. II. Analytical model

DOE PAGES

Khrabry, A.; Kaganovich, I. D.; Nemchinsky, V.; ...

2018-01-22

A short atmospheric pressure argon arc is studied numerically and analytically. In a short arc with an inter-electrode gap of several millimeters, non-equilibrium effects in plasma play an important role in operation of the arc. High anode temperature leads to electron emission and intensive radiation from its surface. A complete, self-consistent analytical model of the whole arc comprising of models for near-electrode regions, arc column, and a model of heat transfer in cylindrical electrodes was developed. The model predicts the width of non-equilibrium layers and arc column, voltages and plasma profiles in these regions, and heat and ion fluxes tomore » the electrodes. Parametric studies of the arc have been performed for a range of the arc current densities, inter-electrode gap widths, and gas pressures. The model was validated against experimental data and verified by comparison with numerical solution. In conclusion, good agreement between the analytical model and simulations and reasonable agreement with experimental data were obtained.« less

Investigation of the short argon arc with hot anode. II. Analytical model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Khrabry, A.; Kaganovich, I. D.; Nemchinsky, V.

A short atmospheric pressure argon arc is studied numerically and analytically. In a short arc with an inter-electrode gap of several millimeters, non-equilibrium effects in plasma play an important role in operation of the arc. High anode temperature leads to electron emission and intensive radiation from its surface. A complete, self-consistent analytical model of the whole arc comprising of models for near-electrode regions, arc column, and a model of heat transfer in cylindrical electrodes was developed. The model predicts the width of non-equilibrium layers and arc column, voltages and plasma profiles in these regions, and heat and ion fluxes tomore » the electrodes. Parametric studies of the arc have been performed for a range of the arc current densities, inter-electrode gap widths, and gas pressures. The model was validated against experimental data and verified by comparison with numerical solution. In conclusion, good agreement between the analytical model and simulations and reasonable agreement with experimental data were obtained.« less

Rapid determination of minoxidil in human plasma using ion-pair HPLC.

PubMed

Zarghi, A; Shafaati, A; Foroutan, S M; Khoddam, A

2004-10-29

A rapid, simple and sensitive ion-pair high-performance liquid chromatography (HPLC) method has been developed for quantification of minoxidil in plasma. The assay enables the measurement of minoxidil for therapeutic drug monitoring with a minimum detectable limit of 0.5 ng ml(-1). The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was performed on an analytical 150 x 4.6 mm i.d. microbondapak C18 column. The wavelength was set at 281 nm. The mobile phase was a mixture of 0.01 M sodium dihydrogen phosphate buffer and acetonitrile (60:40, v/v) containing 2.5 mM sodium dodecyl sulphate adjusted to pH 3.5 at a flow rate of 1 ml/min. The column temperature was set at 50 degrees C. The calibration curve was linear over the concentration range 2-100 ng ml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 8%.

Multi-component immunoaffinity subtraction chromatography: an innovative step towards a comprehensive survey of the human plasma proteome.

PubMed

Pieper, Rembert; Su, Qin; Gatlin, Christine L; Huang, Shih-Ting; Anderson, N Leigh; Steiner, Sandra

2003-04-01

In order to discover novel protein markers indicative of disease processes or drug effects, the proteomics technology platform most commonly used consists of high resolution protein separation by two-dimensional electrophoresis (2-DE), mass spectrometric identification of proteins from stained gel spots and a bioinformatic data analysis process supported by statistics. This approach has been more successful in profiling proteins and their disease- or treatment-related quantitative changes in tissue homogenates than in plasma samples. Plasma protein display and quantitation suffer from several disadvantages: very high abundance of a few proteins; high heterogeneity of many proteins resulting in long charge trains; crowding of 2-DE separated protein spots in the molecular mass range between 45-80 kD and in the isoelectric point range between 4.5 and 6. Therefore, proteomic technologies are needed that address these problems and particularly allow accurate quantitation of a larger number of less abundant proteins in plasma and other body fluids. The immunoaffinity-based protein subtraction chromatography (IASC) described here removes multiple proteins present in plasma and serum in high concentrations effectively and reproducibly. Applying IASC as an upfront plasma sample preparation process for 2-DE, the protein spot pattern observed in gels changes dramatically and at least 350 additional lower abundance proteins are visualized. Affinity-purified polyclonal antibodies (pAbs) are the immunoaffinity reagents used to specifically remove the abundant proteins such as albumin, immunoglobulin G, immunoglobulin A, transferrin, haptoglobin, alpha-1-antitrypsin, hemopexin, transthyretin, alpha-2-HS glycoprotein, alpha-1-acid glycoprotein, alpha-2-macroglobulin and fibrinogen from human plasma samples. To render the immunoaffinity subtraction procedure recyclable, the pAbs are immobilized and cross-linked on chromatographic matrices. Antibody-coupled matrices specific for one protein each can be pooled to form mixed-bed IASC columns. We show that up to ten affinity-bound plasma proteins with similar solubility characteristics are eluted from a mixed-bed column in one step. This facilitates automated chromatographic processing of plasma samples in high throughput, which is desirable in proteomic disease marker discovery projects.

Generator of chemically active low-temperature plasma

NASA Astrophysics Data System (ADS)

Tyuftyaev, A. S.; Gadzhiev, M. Kh; Sargsyan, M. A.; Demirov, N. A.; Spector, N. O.

2016-11-01

A new generator of high enthalpy (H 0 > 40 kJ/g), chemically active nitrogen and air plasmas was designed and constructed. Main feature of the generator is an expanding channel of an output electrode; the generator belongs to the class of DC plasma torches with thermionic cathode with an efficiency of 80%. The generator ensures the formation of a slightly divergent plasma jet (2α = 12°) with a diameter of D = 10-12 mm, an electric arc maximum power of 20-50 kW, plasma forming gas flow rate 1.0-2.0 g/s, and the average plasma temperature at an outlet of 8000-11000 K.

Semiconductor radiation detector

DOEpatents

Bell, Zane W.; Burger, Arnold

2010-03-30

A semiconductor detector for ionizing electromagnetic radiation, neutrons, and energetic charged particles. The detecting element is comprised of a compound having the composition I-III-VI.sub.2 or II-IV-V.sub.2 where the "I" component is from column 1A or 1B of the periodic table, the "II" component is from column 2B, the "III" component is from column 3A, the "IV" component is from column 4A, the "V" component is from column 5A, and the "VI" component is from column 6A. The detecting element detects ionizing radiation by generating a signal proportional to the energy deposited in the element, and detects neutrons by virtue of the ionizing radiation emitted by one or more of the constituent materials subsequent to capture. The detector may contain more than one neutron-sensitive component.

Radio frequency discharge with control of plasma potential distribution.

PubMed

Dudnikov, Vadim; Dudnikov, A

2012-02-01

A RF discharge plasma generator with additional electrodes for independent control of plasma potential distribution is proposed. With positive biasing of this ring electrode relative end flanges and longitudinal magnetic field a confinement of fast electrons in the discharge will be improved for reliable triggering of pulsed RF discharge at low gas density and rate of ion generation will be enhanced. In the proposed discharge combination, the electron energy is enhanced by RF field and the fast electron confinement is improved by enhanced positive plasma potential which improves the efficiency of plasma generation significantly. This combination creates a synergetic effect with a significantly improving the plasma generation performance at low gas density. The discharge parameters can be optimized for enhance plasma generation with acceptable electrode sputtering.

Method for plasma formation for extreme ultraviolet lithography-theta pinch

DOEpatents

Hassanein, Ahmed [Naperville, IL; Konkashbaev, Isak [Bolingbrook, IL; Rice, Bryan [Hillsboro, OR

2007-02-20

A device and method for generating extremely short-wave ultraviolet electromagnetic wave, utilizing a theta pinch plasma generator to produce electromagnetic radiation in the range of 10 to 20 nm. The device comprises an axially aligned open-ended pinch chamber defining a plasma zone adapted to contain a plasma generating gas within the plasma zone; a means for generating a magnetic field radially outward of the open-ended pinch chamber to produce a discharge plasma from the plasma generating gas, thereby producing a electromagnetic wave in the extreme ultraviolet range; a collecting means in optical communication with the pinch chamber to collect the electromagnetic radiation; and focusing means in optical communication with the collecting means to concentrate the electromagnetic radiation.

Method for the chemical separation of GE-68 from its daughter Ga-68

DOEpatents

Fitzsimmons, Jonathan M.; Atcher, Robert W.

2010-06-01

The present invention is directed to a generator apparatus for separating a daughter gallium-68 radioisotope substantially free of impurities from a parent gernanium-68 radioisotope, including a first resin-containing column containing parent gernanium-68 radioisotope and daughter gallium-68 radioisotope, a source of first eluent connected to said first resin-containing column for separating daughter gallium-68 radioisotope from the first resin-containing column, said first eluent including citrate whereby the separated gallium is in the form of gallium citrate, a mixing space connected to said first resin-containing column for admixing a source of hydrochloric acid with said separated gallium citrate whereby gallium citrate is converted to gallium tetrachloride, a second resin-containing column for retention of gallium-68 tetrachloride, and, a source of second eluent connected to said second resin-containing column for eluting the daughter gallium-68 radioisotope from said second resin-containing column.

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins.

PubMed

Kurdi, Said El; Muaileq, Dina Abu; Alhazmi, Hassan A; Bratty, Mohammed Al; Deeb, Sami El

2017-06-27

HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.

High-efficiency liquid chromatography on conventional columns and instrumentation by using temperature as a variable I. Experiments with 25 cm x 4.6 mm I.D., 5 microm ODS columns.

PubMed

Lestremau, François; Cooper, Andrew; Szucs, Roman; David, Frank; Sandra, Pat

2006-03-24

High plate numbers were obtained in conventional LC by coupling columns and by using temperature to reduce the viscosity of the mobile phase. At 80 degrees C up to eight columns of 25 cm x 4.6 mm I.D. packed with 5 microm ODS particles could be coupled generating 180,000 effective plates while the pressure drop was only 350bar. For routine work, a set of four columns is preferred. The analysis times on one column operated at 30 degrees C and 1 mL/min flow rate and on four columns at 80 degrees C and 2 mL/min flow rate are the same in isoeluotropic conditions while the resolution is doubled. Multicolumn systems were successfully applied in isocratic and gradient mode for the analysis of pharmaceutical and environmental samples.

Determination of the sulfate and glucuronide conjugates of levornidazole in human plasma and urine, and levornidazole and its five metabolites in human feces by high performance liquid chromatography-tandem mass spectrometry.

PubMed

He, Gaoli; Guo, Beining; Zhang, Jing; Li, Yi; Wu, Xiaojie; Fan, Yaxin; Chen, Yuancheng; Cao, Guoying; Yu, Jicheng

2018-04-01

Levornidazole is a novel third-generation nitroimidazoles antibiotic which metabolism and disposition in human are not well known. We have previously developed two methods to quantify levornidazole and its phase I metabolites, Ml (Hydroxylation metabolite), M2 (N-dealkylation metabolite) and M4 (Oxidative dechlorination metabolite), in human plasma and urine. In this study, we developed three novel liquid chromatographic-tandem mass spectrometric (LC-MS/MS) methods and analyzed its phase II metabolites, sulfate conjugate (M6) and glucuronide conjugate (M16), in human plasma and urine, and the parent drug and above-mentioned five metabolites in human feces samples. Analytes and internal standard (IS) in human plasma were extracted by a solid-phase extraction procedure and separated on an ACQUITY UPLC CSH C18 column in gradient elution using acetonitrile and 0.1% formic acid aqueous solution as the mobile phase. The pretreatment procedures for urine and feces homogenate samples involved a protein precipitation followed by liquid-liquid extraction, and chromatographic separations were performed on the Atlantis T3 columns of different lengths and particle sizes (2.1 × 50 mm, 3 μm and 2.1 × 150 mm, 5 μm), respectively. The mobile phases consisted of formic acid and acetonitrile-methanol solution (v/v, 50:50) in gradient elution. The MS/MS analysis was conducted on TSQ Quantum triple quadrupole mass spectrometer using electrospray ionization with selected reaction monitoring (SRM) in the positive ion mode. The calibration curves for all analytes were linear and the validation ranges were as follows: 0.005-0.500 μg/mL for M6 and 0.005-2.500 μg/mL for M16 in plasma; 0.010-10.000 μg/mL for M6 and M16 in urine; 0.005-1.000 μg/mL for levornidazole, M2, M4 and M16, and 0.010-2.000 μg/mL for M1 and M6 in human feces homogenate. Across these matrices, mean intra- and inter- batch accuracy values were in the ranges of 80.0%-120.0%, and intra- and inter-batch precision values did not exceed 20%. It was fully validated including selectivity, linearity, matrix effect, extraction recovery, stability, dilution integrity, carryover and incurred sample analysis (ISR). These newly developed methods were successfully applied in pharmacokinetics, metabolism and disposition study of levornidazole in 12 healthy Chinese subjects. Copyright © 2018 Elsevier B.V. All rights reserved.

QSPR models for predicting generator-column-derived octanol/water and octanol/air partition coefficients of polychlorinated biphenyls.

PubMed

Yuan, Jintao; Yu, Shuling; Zhang, Ting; Yuan, Xuejie; Cao, Yunyuan; Yu, Xingchen; Yang, Xuan; Yao, Wu

2016-06-01

Octanol/water (K(OW)) and octanol/air (K(OA)) partition coefficients are two important physicochemical properties of organic substances. In current practice, K(OW) and K(OA) values of some polychlorinated biphenyls (PCBs) are measured using generator column method. Quantitative structure-property relationship (QSPR) models can serve as a valuable alternative method of replacing or reducing experimental steps in the determination of K(OW) and K(OA). In this paper, two different methods, i.e., multiple linear regression based on dragon descriptors and hologram quantitative structure-activity relationship, were used to predict generator-column-derived log K(OW) and log K(OA) values of PCBs. The predictive ability of the developed models was validated using a test set, and the performances of all generated models were compared with those of three previously reported models. All results indicated that the proposed models were robust and satisfactory and can thus be used as alternative models for the rapid assessment of the K(OW) and K(OA) of PCBs. Copyright © 2016 Elsevier Inc. All rights reserved.

TEFLON BELLOWS PULSE GENERATORS FOR SOLVENT EXTRACTION PULSE COLUMNS

DOE Office of Scientific and Technical Information (OSTI.GOV)

McCarthy, P.B.

1954-01-01

A Teflon bellows-type pulse generator is described which consists of two 3-in. nominal diameter Teflon bellows mounted on either end of a flanged spool piece and rigidly connected internally by a push rod so that the two of bellows move in tandem. The ends of the two bellows are closed by means of blind flanges. Tne spool piece is designed for insertion in a 6-in. diameter nozzle on a solvent extraction pulse column. The double bellows arrangement constitutes a safety feature to prevent loss of the column contents in the event of failure of the inner bellows in contact withmore » column solution. Failure of the inner bellows may be detected by a conductivity probe mounted in the air space inside of the double bellows assembly. Reciprocating motion is imcrank arm rigidly connected through a cross head and push rod to the face of the external bellows flange. The push rod is guided by means of linear ball bushings. Frequency variation over a range of 30 to 100 cycles/ min.was obtained by use of a Thymotrol-controlled electric motor to drive the crank arm. Variable stroke adjustment (0 to 1-in. range) was possible by adjustment of linkages on the crank arm. A load compensating spring was founnd desirable to counteract the thrust on the push rod resulting tom the static pressure at the bottom of the solvent extraction column. Without the spring, accelerated wear of the bearing on the crank arm occured. The pulse generator operated uneventfully for 1776 hours (6.61x lO/sup 6/ cycles) at a frequency of 62 cycles/min. and a bellows travel of l-in. (equivalent to a displacement of 1.6 in. in a 3-in. diam. column). (auth)« less

Determination of molindone enantiomers in human plasma by high-performance liquid chromatography-tandem mass spectrometry using macrocyclic antibiotic chiral stationary phases.

PubMed

Jiang, Hongliang; Li, Yinghe; Pelzer, Mary; Cannon, Michelle J; Randlett, Christopher; Junga, Heiko; Jiang, Xiangyu; Ji, Qin C

2008-05-30

A sensitive and selective bioanalytical assay was developed and validated for the determination of enantiomeric molindone in human plasma using high-performance liquid chromatography-tandem mass spectrometry along with supported liquid extraction procedures. The chiral separation was evaluated and optimized on macrocyclic antibiotic type chiral stationary phases (CSPs) based on teicoplanin aglycone (Chirobiotic TAG) in polar organic, polar ionic, and reversed-phase mode chromatography, respectively. Complete baseline separation was achieved on a Chirobiotic TAG column under isocratic condition in reversed-phase chromatography. The method validation was conducted using a Chirobiotic TAG column (100 mm x 2.1 mm) over the curve range 0.100-100 ng/ml for each molindone enantiomer using 0.0500 ml of plasma sample. The flow rate was 0.8 ml/min and the total run time was 9 min. Supported liquid extraction in a 96-well plate format was used for sample preparation. Parameters including recovery, matrix effect, linearity, sensitivity, specificity, carryover, precision, accuracy, dilution integrity, and stability were evaluated. The intra- and inter-day precision and accuracy of the quality control samples at low, medium, and high concentration levels were RSD

HPLC with fluorescence detection assay of perampanel, a novel AMPA receptor antagonist, in human plasma for clinical pharmacokinetic studies.

PubMed

Mano, Yuji; Takenaka, Osamu; Kusano, Kazutomi

2015-10-01

Perampanel (Fycompa®), a novel α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonist, is registered for the adjunctive treatment of patients (aged ≥12 years) with refractory partial-onset seizures. To support therapeutic drug monitoring, a simple high-performance liquid chromatography (HPLC) assay with fluorescence detection was developed to determine perampanel concentrations in human plasma and validated to support clinical trials. Human plasma samples (1.0 mL) were processed by liquid extraction using diethyl ether, followed by chromatographic separation on a YMC Pack Pro C18 column (150 × 4.6 mm i.d., 5 µm) with isocratic elution of acetonitrile-water-acetic acid-sodium acetate (840:560:3:1.8, v/v/v/w) at a flow rate of 1.0 mL/min. Column eluent was monitored at excitation and emission wavelengths of 290 and 430 nm, respectively. The assay was linear (range 1.0-500 ng/mL) and this could be extended to 25 µg/mL by 50-fold dilution integrity. No endogenous peaks were detected in the elution of analytes in drug-free blank human plasma from six individuals and no interference was observed with co-medications tested. Intra- and inter-batch reproducibility studies demonstrated accuracy and precision within the acceptance criteria of bioanalytical guidelines. Validation data demonstrated that our assay is simple, selective, reproducible and suitable for therapeutic drug monitoring of perampanel. Copyright © 2015 John Wiley & Sons, Ltd.

On-line solid phase extraction using the Prospekt-2 coupled with a liquid chromatography/tandem mass spectrometer for the determination of dextromethorphan, dextrorphan and guaifenesin in human plasma.

PubMed

Kuhlenbeck, Debbie L; Eichold, Thomas H; Hoke, Steven H; Baker, Timothy R; Mensen, Robert; Wehmeyer, Kenneth R

2005-01-01

An on-line liquid chromatography/tandem mass spectrometry (LC-MS/MS) procedure, using the Prospekt- 2 system, was developed and used for the determination of the levels of the active ingredients of cough/cold medications in human plasma matrix. The experimental configuration allows direct plasma injection by performing on- line solid phase extraction (SPE) on small cartridge columns prior to elution of the analyte(s) onto the analytical column and subsequent MS/MS detection. The quantitative analysis of three analytes with differing polarities, dextromethorphan (DEX), dextrorphan (DET) and guaifenesin (GG) in human plasma presented a significant challenge. Using stable-isotope-labeled internal standards for each analyte, the Prospekt-2 on-line methodology was evaluated for sensitivity, suppression, accuracy, precision, linearity, analyst time, analysis time, cost, carryover and ease of use. The lower limit of quantitation for the on-line SPE procedure for DEX, DET and GG was 0.05, 0.05 and 5.0 ng mL(-1), respectively, using a 0.1 mL sample volume. The linear range for DEX and DET was 0.05-50 ng mL(-1) and was 5-5,000 ng mL(-1) for GG. Accuracy and precision data for five different levels of QC samples were collected over three separate days. Accuracy ranged from 90% to 112% for all three analytes, while the precision, as measured by the %RSD, ranged from 1.5% to 16.0%

Determination of doxorubicin in plasma by using CE coupled with in-column tapered optic-fiber light-emitting diode induced fluorescence detection.

PubMed

Yang, Xiupei; Qian, Fan; Xie, Linxiang; Yang, Xiaocui; Cheng, Xiumei; Choi, Martin M F

2014-03-01

This paper proposes a novel strategy to enhance detection of doxorubicin in human plasma, using homemade CE combined with normal stacking mode (NSM). The detection system of CE named as in-column tapered optic-fiber light-emitting diode induced fluorescence detection system is economic and more sensitive that has been demonstrated in our previous work. The influence of sample matrix, BGE, applied voltage, and injection time on the efficiency of NSM were systematically investigated. The clean extracts were subjected to CE separation with optimal experimental conditions: Ethanol-water (1:1, v/v) was used as sample matrix, pH 4.12 15 mM sodium phosphate buffer solution containing 70% v/v ACN, applied voltage 23 kV and 45 s hydrodynamic injection at a height of 20 cm. The detection system displayed linear dynamic range from 6.4 to 1.13 × 10(3) ng/mL with a correlation coefficient of 0.9990 and LOD 2.2 ng/mL for doxorubicin (DOX). The proposed CE method has been successfully applied to determine DOX in human plasma which the recoveries of standard DOX added to human plasma were found to been the range of 93.8-104.6%. The results obtained demonstrate that our detection system combined with NSM is a good idea to enhance sensitivity in CE for routine determination of DOX in some biological specimens. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Liquid chromatographic extraction medium

DOEpatents

Horwitz, E.P.; Dietz, M.L.

1994-09-13

A method and apparatus are disclosed for extracting strontium and technetium values from biological, industrial and environmental sample solutions using a chromatographic column. An extractant medium for the column is prepared by generating a solution of a diluent containing a Crown ether and dispersing the solution on a resin substrate material. The sample solution is highly acidic and is introduced directed to the chromatographic column and strontium or technetium is eluted using deionized water. 1 fig.

Vacuum arc plasma thrusters with inductive energy storage driver

NASA Technical Reports Server (NTRS)

Krishnan, Mahadevan (Inventor)

2009-01-01

A plasma thruster with a cylindrical inner and cylindrical outer electrode generates plasma particles from the application of energy stored in an inductor to a surface suitable for the formation of a plasma and expansion of plasma particles. The plasma production results in the generation of charged particles suitable for generating a reaction force, and the charged particles are guided by a magnetic field produced by the same inductor used to store the energy used to form the plasma.

Determination of salicylic acid by HPLC in plasma and saliva from children with juvenile chronic arthritis.

PubMed

Legaz, M E; Acitores, E; Valverde, F

1992-12-01

A high performance liquid chromatography (HPLC) method has been developed for measuring salicylic acid in the plasma and saliva of children with juvenile chronic arthritis (JCA). Samples were extracted with diethyl ether and, after drying, redissolved in methanol to be chromatographed. Quantitation of salicylic acid was performed by reverse phase HPLC on a spherisorb ODS-2 column, using methanol: water: acetic acid as mobile phase. Phenolic was monitored by absorbance at 237 nm. Linearity between the amount of mass injected and the response in the detector was determined. This method was applied to compare concentrations of salivary and plasma salicylic acid. The method also permitted the quantitation of salivary salicylate as a non-invasive, indirect method for monitoring the concentration of plasma salicylate in patients with JCA.

Shielded beam delivery apparatus and method

DOEpatents

Hershcovitch, Ady; Montano, Rory Dominick

2006-07-11

An apparatus includes a plasma generator aligned with a beam generator for producing a plasma to shield an energized beam. An electrode is coaxially aligned with the plasma generator and followed in turn by a vortex generator coaxially aligned with the electrode. A target is spaced from the vortex generator inside a fluid environment. The electrode is electrically biased relative to the electrically grounded target for driving the plasma toward the target inside a vortex shield.

Validation and implementation of liquid chromatographic-mass spectrometric (LC-MS) methods for the quantification of tenofovir prodrugs.

PubMed

Hummert, Pamela; Parsons, Teresa L; Ensign, Laura M; Hoang, Thuy; Marzinke, Mark A

2018-04-15

The nucleotide reverse transcriptase inhibitor tenofovir (TFV) is widely administered in a disoproxil prodrug form (tenofovir disoproxil fumarate, TDF) for HIV management and prevention. Recently, novel prodrugs tenofovir alafenamide fumarate (TAF) and hexadecyloxypropyl tenofovir (CMX157) have been pursued for HIV treatment while minimizing adverse effects associated with systemic TFV exposure. Dynamic and sensitive bioanalytical tools are required to characterize the pharmacokinetics of these prodrugs in systemic circulation. Two parallel methods have been developed, one to combinatorially quantify TAF and TFV, and a second method for CMX157 quantification, in plasma. K 2 EDTA plasma was spiked with TAF and TFV, or CMX157. Following the addition of isotopically labeled internal standards and sample extraction via solid phase extraction (TAF and TFV) or protein precipitation (CMX157), samples were subjected to liquid chromatographic-tandem mass spectrometric (LC-MS/MS) analysis. For TAF and TFV, separation occurred using a Zorbax Eclipse Plus C18 Narrow Bore RR, 2.1 × 50 mm, 3.5 μm column and analytes were detected on an API5000 mass analyzer; CMX157 was separated using a Kinetex C8, 2.1 × 50 mm, 2.6 μm column and quantified using an API4500 mass spectrometer. Methods were validated according to FDA Bioanalytical Method Validation guidelines. Analytical methods: were optimized for the multiplexed monitoring of TAF and TFV, and CMX157 in plasma. The lower limits of quantification (LLOQs) for TAF, TFV, and CMX157 were 0.03, 1.0, and 0.25 ng/mL, respectively. Calibration curves were generated via weighted linear regression of standards. Intra- and inter-assay precision and accuracy studies demonstrated %CVs ≤ 14.4% and %DEVs ≤ ± 7.95%, respectively. Stability and matrix effects studies were also performed. All results were acceptable and in accordance with the recommended guidelines for bioanalytical methods. Assays were also applied to quantify in vivo concentrations of prodrugs and TFV in a preclinical study post-rectal administration. Sensitive, specific, and dynamic LC-MS/MS assays have been developed and validated for the multiplexed quantification TAF and TFV, as well as an independent assay for CMX157 quantification, in plasma. The described methods meet sufficient throughput criteria to support large research trials. Copyright © 2018 Elsevier B.V. All rights reserved.

Fundamental and Applied Investigations in Atomic Spectrometric Analysis

NASA Astrophysics Data System (ADS)

Wu, Min

Simultaneous laser-excited fluorescence and absorption measurements were performed and the results have revealed that any interference caused by easily ionized elements does not originate from variations in analyte emission (quantum) efficiency. A closely related area, the roles of wet and dry aerosols in the matrix interference are clarified through spatially resolved imaging of the plasma by a charged coupled device camera. To eliminate matrix interference effects practically, various methods have been developed based on the above studies. The use of column pre-concentration with flow injection analysis has been found to provide a simple solution for reducing interference effects and increasing sensitivity of elemental analysis. A novel mini-spray chamber was invented. The new vertical rotary spray chamber combines gravitational, centrifugal, turbulent, and impact droplet segregation mechanisms to achieve a higher efficiency of small-droplet formation in a nebulized sample spray. As a result, it offers also higher sample-transport efficiency, lower memory effects, and improved analytical figures of merit over existing devices. This new device was employed with flow injection analysis to simulate an interface for coupling high performance liquid chromatography (HPLC) to a microwave plasma for chromatographic detection. The detection limits for common metallic elements are in the range of 5-50 mug/mL, and are degraded only twofold when the elements are presented in an organic solvent such as ethanol or methanol. Other sample-introduction schemes have also been investigated to improve sample-introduction technology. The direct coupling of hydride-generation techniques to the helium microwave plasma torch was evaluated for the determination of arsenic, antimony and tin by atomic emission spectrometry. A manually controlled peristaltic pump was modified for computer control and continuous flow injection was evaluated for standard calibration and trace elemental analysis. The present work evaluates the coupling of a novel microwave plasma torch with a quadruple mass spectrometer for the detection of ionic species from different nonmetals. Initial work performed with such a combination is demonstrated to be not only practicable but also promising. Detection limits for the halogens (F, Cl, Br, I) and S are in the range between 10 ng/mL and 1mug/mL. Further improvements have been realized through the use of chemical -vapor generation and by optimization of the plasma and the mass spectrometer. (Abstract shortened by UMI.).

Dynamic Pressure Calibration Standard

NASA Technical Reports Server (NTRS)

Schutte, P. C.; Cate, K. H.; Young, S. D.

1986-01-01

Vibrating columns of fluid used to calibrate transducers. Dynamic pressure calibration standard developed for calibrating flush diaphragm-mounted pressure transducers. Pressures up to 20 kPa (3 psi) accurately generated over frequency range of 50 to 1,800 Hz. System includes two conically shaped aluminum columns one 5 cm (2 in.) high for low pressures and another 11 cm (4.3 in.) high for higher pressures, each filled with viscous fluid. Each column mounted on armature of vibration exciter, which imparts sinusoidally varying acceleration to fluid column. Signal noise low, and waveform highly dependent on quality of drive signal in vibration exciter.

Impact of plasma jet vacuum ultraviolet radiation on reactive oxygen species generation in bio-relevant liquids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jablonowski, H.; Hammer, M. U.; Reuter, S.

Plasma medicine utilizes the combined interaction of plasma produced reactive components. These are reactive atoms, molecules, ions, metastable species, and radiation. Here, ultraviolet (UV, 100–400 nm) and, in particular, vacuum ultraviolet (VUV, 10–200 nm) radiation generated by an atmospheric pressure argon plasma jet were investigated regarding plasma emission, absorption in a humidified atmosphere and in solutions relevant for plasma medicine. The energy absorption was obtained for simple solutions like distilled water (dH{sub 2}O) or ultrapure water and sodium chloride (NaCl) solution as well as for more complex ones, for example, Rosewell Park Memorial Institute (RPMI 1640) cell culture media. As moderate stablemore » reactive oxygen species, hydrogen peroxide (H{sub 2}O{sub 2}) was studied. Highly reactive oxygen radicals, namely, superoxide anion (O{sub 2}{sup •−}) and hydroxyl radicals ({sup •}OH), were investigated by the use of electron paramagnetic resonance spectroscopy. All species amounts were detected for three different treatment cases: Plasma jet generated VUV and UV radiation, plasma jet generated UV radiation without VUV part, and complete plasma jet including all reactive components additionally to VUV and UV radiation. It was found that a considerable amount of radicals are generated by the plasma generated photoemission. From the experiments, estimation on the low hazard potential of plasma generated VUV radiation is discussed.« less

Simultaneous analysis of retinol, all-trans- and 13-cis-retinoic acid and 13-cis-4-oxoretinoic acid in plasma by liquid chromatography using on-column concentration after single-phase fluid extraction.

PubMed

Teerlink, T; Copper, M P; Klaassen, I; Braakhuis, B J

1997-06-20

A reversed-phase high-performance liquid chromatographic method for the simultaneous analysis of retinol, all-trans-retinoic acid, 13-cis-retinoic acid and 13-cis-4-oxoretinoic acid in human plasma and cell culture medium is described. Sample preparation involves precipitation of proteins and extraction of retinoids with 60% acetonitrile. After centrifugation, the acetonitrile content of the supernatant is reduced to 45%, allowing on-column concentration of analytes. Injection volumes up to 2.0 ml (equivalent to 0.525 ml of sample) can be used without compromising chromatographic resolution of all-trans-retinoic acid and 13-cis-retinoic acid. Retinoids were stable in this extract and showed no isomerization when stored in the dark in a cooled autosampler, allowing automated analysis of large series of samples. Recoveries from spiked plasma samples were between 95 and 103%. Although no internal standard was used, the inter-assay precision for all retinoids was better than 6% and 4% at concentrations of 30 nM and 100 nM, respectively. The method is a valuable tool for the study of cellular metabolism of all-trans-retinoic acid, as polar metabolites of this compound can be detected with high sensitivity in cell culture media.

Determination of L-ephedrine, pseudoephedrine, and caffeine in rat plasma by liquid chromatography-tandem mass spectrometry.

PubMed

Cooper, Stephen D; Fletcher, Brenda L; Silinski, Melanie A Rehder; Brown, Sherri S; Lodge, Jon W; Fernando, Reshan A; Collins, Bradley J

2011-07-01

A rapid and simple liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of L-ephedrine, pseudoephedrine, and caffeine in male Fisher-344 rat plasma at nanogram-per-milliliter concentrations for use in support of toxicology studies. Only 25 μL of plasma is required, and extraction is performed using a simple, single-step protein precipitation. The method was validated over a range of 2.09 to 5460 ng/mL for L-ephedrine, 2.09 to 5050 ng/mL for pseudoephedrine and 2.03 to 5340 ng/mL for caffeine. A binary gradient elution at 0.3 mL/min was used with a Waters XBridge Phenyl (2.1 × 150 mm, 3.5 μm) column and a Waters XBridge Phenyl 2.1- × 10-mm guard column at ambient temperature. The mobile phase consisted of 10 mM ammonium acetate in water (pH 5.0) and methanol. Caffeine trimethyl-(13)C(3) was used as the internal standard. The method was evaluated for linearity, recovery, precision, accuracy, and stability, and it was successfully applied in toxicokinetic studies of ephedrine, administered alone, in combination with caffeine, and in the herbal source Ma Huang.

Development and validation of an HPLC method for simultaneous determination of trimethoprim and sulfamethoxazole in human plasma.

PubMed

Sayar, Esin; Sahin, Selma; Cevheroglu, Semsettin; Hincal, A Atilla

2010-09-01

The combination of trimethoprim (TMP) and sulfamethoxazole (SMX) is used in the treatment of many common infections such as urinary, respiratory and gastrointestinal tract infections. The aim of this study was to determine TMP and SMX simultaneously in human plasma samples by high performance liquid chromatography (HPLC) using antipyrine as the internal standard. Separation of the compounds was achieved on a reverse-phase C8 column packed with 5 microm dimethyl octadecylsilyl bonded amorphous silica (4.6 mm x 250 mm) column using a mobile phase consisted of potassium hydrogen phosphate, acetonitrile, methanol and water adjusted to pH 6.2. The mobile phase was delivered at a flow rate of 1 mL min- and the effluent was monitored using Max plot technique at 25 derees C. Retention times were 5 min for TMP, 7 min for antipyrine and 9 min for SMX. Quantitation limits were 10 ng mL(-1) for TMP and 50 ng mL(-1) for SMX. Our findings indicated that the developed HPLC method was precise, accurate, specific and sensitive for simultaneous determination of TMP and SMX. Proposed HPLC method was successfully applied for the analysis of TMP and SMX in human plasma after oral administration of a co-trimoxazole tablet to human volunteers.

Internal waves interacting with particles in suspension

NASA Astrophysics Data System (ADS)

Micard, Diane

2016-04-01

Internal waves are produced as a consequence of the dynamic balance between buoy- ancy and gravity forces when a particle of fluid is vertically displaced in a stable stratified environment. Geophysical systems such as ocean and atmosphere are naturally stratified and therefore suitable for internal waves to propagate. Furthermore, these two environ- ments stock a vast amount of particles in suspension, which present a large spectrum of physical properties (size, density, shape), and can be organic, mineral or pollutant agents. Therefore, it is reasonable to expect that internal waves will have an active effect over the dynamics of these particles. In order to study the interaction of internal waves and suspended particles, an ide- alized experimental setup has been implemented. A linear stratification is produced in a 80×40×17 cm3 tank, in which two dimensional plane waves are created thanks to the inno- vative wave generator GOAL. In addition, a particle injector has been developed to produce a vertical column of particles within the fluid, displaying the same two-dimensional sym- metry as the waves. The particle injector allows to control the volumic fraction of particles and the size of the column. The presence of internal waves passing through the column of particles allowed to observe two main effects: The column oscillates around an equilibrium position (which is observed in both, the contours an the interior of the column), and the column is displaced as a whole. The column is displaced depending on the characteristics of the column, the gradient of the density, and the intensity and frequency of the wave. When displaced, the particles within the column are sucked towards the source of waves. The direction of the displacement of the column is explained by computing the effect of the Lagrangian drift generated by the wave over the time the particles stay in the wave beam before settling.

High frequency plasma generator for ion thrusters

NASA Technical Reports Server (NTRS)

Goede, H.; Divergilio, W. F.; Fosnight, V. V.; Komatsu, G.

1984-01-01

The results of a program to experimentally develop two new types of plasma generators for 30 cm electrostatic argon ion thrusters are presented. The two plasma generating methods selected for this study were by radio frequency induction (RFI), operating at an input power frequency of 1 MHz, and by electron cyclotron heating (ECH) at an operating frequency of 5.0 GHz. Both of these generators utilize multiline cusp permanent magnet configurations for plasma confinement and beam profile optimization. The program goals were to develop a plasma generator possessing the characteristics of high electrical efficiency (low eV/ion) and simplicity of operation while maintaining the reliability and durability of the conventional hollow cathode plasma sources. The RFI plasma generator has achieved minimum discharge losses of 120 eV/ion while the ECH generator has obtained 145 eV/ion, assuming a 90% ion optical transparency of the electrostatic acceleration system. Details of experimental tests with a variety of magnet configurations are presented.

Developing procedures for the large-scale purification of human serum butyrylcholinesterase.

PubMed

Saxena, Ashima; Luo, Chunyuan; Doctor, Bhupendra P

2008-10-01

Human serum butyrylcholinesterase (Hu BChE) is the most viable candidate for the prophylactic treatment of organophosphate poisoning. A dose of 200 mg/70 kg is predicted to protect humans against 2x LD(50) of soman. Therefore, the aim of this study was to develop procedures for the purification of gram quantities of this enzyme from outdated human plasma or Cohn Fraction IV-4. The purification of Hu BChE was accomplished by batch adsorption on procainamide-Sepharose-CL-4B affinity gel followed by ion-exchange chromatography on a DEAE-Sepharose column. For the purification of enzyme from Cohn Fraction IV-4, it was resuspended in 25 mM sodium phosphate buffer, pH 8.0, and fat was removed by decantation, prior to batch adsorption on procainamide-Sepharose gel. In both cases, the procainamide gel was thoroughly washed with 25 mM sodium phosphate buffer, pH 8.0, containing 0.05 M NaCl, and the enzyme was eluted with the same buffer containing 0.1 M procainamide. The enzyme was dialyzed and the pH was adjusted to 4.0 before loading on the DEAE column equilibrated in sodium acetate buffer, pH 4.0. The column was thoroughly washed with 25 mM sodium phosphate buffer, pH 8.0 containing 0.05 M NaCl before elution with a gradient of 0.05-0.2M NaCl in the same buffer. The purity of the enzyme following these steps ranged from 20% to 40%. The purity of the enzyme increased to >90% by chromatography on an analytical procainamide affinity column. Results show that Cohn Fraction IV-4 is a much better source than plasma for the large-scale isolation of purified Hu BChE.

Planar cell polarity signaling coordinates oriented cell division and cell rearrangement in clonally expanding growth plate cartilage.

PubMed

Li, Yuwei; Li, Ang; Junge, Jason; Bronner, Marianne

2017-10-10

Both oriented cell divisions and cell rearrangements are critical for proper embryogenesis and organogenesis. However, little is known about how these two cellular events are integrated. Here we examine the linkage between these processes in chick limb cartilage. By combining retroviral-based multicolor clonal analysis with live imaging, the results show that single chondrocyte precursors can generate both single-column and multi-column clones through oriented division followed by cell rearrangements. Focusing on single column formation, we show that this stereotypical tissue architecture is established by a pivot-like process between sister cells. After mediolateral cell division, N-cadherin is enriched in the post-cleavage furrow; then one cell pivots around the other, resulting in stacking into a column. Perturbation analyses demonstrate that planar cell polarity signaling enables cells to pivot in the direction of limb elongation via this N-cadherin-mediated coupling. Our work provides new insights into the mechanisms generating appropriate tissue architecture of limb skeleton.

Planar cell polarity signaling coordinates oriented cell division and cell rearrangement in clonally expanding growth plate cartilage

PubMed Central

Li, Yuwei; Li, Ang; Junge, Jason

2017-01-01

Both oriented cell divisions and cell rearrangements are critical for proper embryogenesis and organogenesis. However, little is known about how these two cellular events are integrated. Here we examine the linkage between these processes in chick limb cartilage. By combining retroviral-based multicolor clonal analysis with live imaging, the results show that single chondrocyte precursors can generate both single-column and multi-column clones through oriented division followed by cell rearrangements. Focusing on single column formation, we show that this stereotypical tissue architecture is established by a pivot-like process between sister cells. After mediolateral cell division, N-cadherin is enriched in the post-cleavage furrow; then one cell pivots around the other, resulting in stacking into a column. Perturbation analyses demonstrate that planar cell polarity signaling enables cells to pivot in the direction of limb elongation via this N-cadherin-mediated coupling. Our work provides new insights into the mechanisms generating appropriate tissue architecture of limb skeleton. PMID:28994649

Method development for speciation analysis of nanoparticle and ionic forms of gold in biological samples by high performance liquid chromatography hyphenated to inductively coupled plasma mass spectrometry

NASA Astrophysics Data System (ADS)

Malejko, Julita; Świerżewska, Natalia; Bajguz, Andrzej; Godlewska-Żyłkiewicz, Beata

2018-04-01

A new method based on coupling high performance liquid chromatography (HPLC) to inductively coupled plasma mass spectrometry (ICP MS) has been developed for the speciation analysis of gold nanoparticles (AuNPs) and dissolved gold species (Au(III)) in biological samples. The column type, the composition and the flow rate of the mobile phase were carefully investigated in order to optimize the separation conditions. The usefulness of two polymeric reversed phase columns (PLRP-S with 100 nm and 400 nm pore size) to separate gold species were investigated for the first time. Under the optimal conditions (PLRP-S400 column, 10 mmol L-1 SDS and 5% methanol as the mobile phase, 0.5 mL min-1 flow rate), detection limits of 2.2 ng L-1 for Au(III), 2.8 ng L-1 for 10 nm AuNPs and 3.7 ng L-1 for 40 nm AuNPs were achieved. The accuracy of the method was proved by analysis of reference material RM 8011 (NIST) of gold nanoparticles of nominal diameter of 10 nm. The HPLC-ICP MS method has been successfully applied to the detection and size characterization of gold species in lysates of green algae Acutodesmus obliquus, typical representative of phytoplankton flora, incubated with 10 nm AuNPs or Au(III).

Removal of Cr(VI) from groundwater by Fe(0)

NASA Astrophysics Data System (ADS)

Gao, Yanjiao; Liu, Rui

2017-11-01

This research was conducted to investigate the treatment of hexavalent chromium (Cr(VI)) by iron powder (Fe(0)) columns of simulated permeable reactive barriers with and without calcium carbonate (CaCO3). Two columns filled with Fe(0) were used as Cr(VI) removal equipment running at a flow velocity of 10 ml/min at room temperature. After 200 days running of the two columns, the results showed that Fe(0) was an effective material for Cr(VI) reduction with an average removal rate of above 84.6%. The performance of Column 2 with CaCO3 was better than Column 1 without CaCO3 in terms of average Cr(VI) removal rate. The presence of CaCO3 buffered the increasing pH caused by Fe(0) corrosion in Column 2 and enhanced the removal rate of Column 2. Scanning Electron Microscopy (SEM) images of Fe(0) in the three stages of running of the two columns illustrated that the coat layer of Column 1 was a little thicker than that of Column 2. Energy-dispersive spectrometry (EDS) results showed that the surface of Fe(0) of Column 2 contained more chromium elements. Raman spectroscopy found that all iron oxide was generated on the Fe(0) surface of Column 1 and Column 2 and chromium class objects were only detected on Fe(0) surface in Column 2.

Dynamic Contraction of the Positive Column of a Self-Sustained Glow Discharge in Molecular Gas Flow

NASA Astrophysics Data System (ADS)

Shneider, Mikhail

2014-10-01

Contraction of the gas discharge, when current contracts from a significant volume of weakly ionized plasma into a thin arc channel, was attracted attention of scientists for more than a century. Studies of the contraction (also called constriction) mechanisms, besides carrying interesting science, are of practical importance, especially when contraction should be prevented. A set of time-dependent two-dimensional equations for the non-equilibrium weakly-ionized nitrogen/ air plasma is formulated. The process is described by a set of time-dependent continuity equations for the electrons, positive and negative ions; gas and vibrational temperature; by taking into account the convective heat and plasma losses by the transverse flux. Transition from the uniform to contracted state was analyzed. It was shown that such transition experiences a hysteresis, and that the critical current of the transition increases when the pressure (gas density) drops. Possible coexistence of the contracted and uniform state of the plasma in the discharge where the current flows along the density gradient of the background gas was discussed. In this talk the problems related to the dynamic contraction of the current channel inside a quasineutral positive column of a self-sustained glow discharge in molecular gas in a rectangular duct with convection cooling will be discussed. Study presented in this talk was stimulated by the fact that there are large number of experiments on the dynamic contraction of a glow discharge in nitrogen and air flows and a many of possible applications. Similar processes play a role in the powerful gas-discharge lasers. In addition, the problem of dynamic contraction in the large volume of non-equilibrium weakly ionized plasma is closely related to the problem of streamer to leader transitions in lightning and blue jets.

Ulcerogenic tumor syndrome of the pancreas associated with a nongastrin acid secretagogue.

PubMed Central

Chey, W Y; Chang, T M; Lee, K Y; Sun, G; Kim, C K; You, C H; Hamilton, D L; Shah, A; Rhee, J C; Mutt, V

1989-01-01

Among 30 patients with islet cell neoplasms or hyperplasia who exhibited marked gastric acid hypersecretion and peptic ulceration and/or diarrhea, fasting plasma gastrin concentrations were less than 150 pg/ml in 11 patients, whereas the remaining 19 patients had hypergastrinemia. Plasma extracts from seven of these 11 patients were assayed for acid secretagogue activity in rats. All seven plasma extracts had secretagogue activity that was not found in the plasma extracts of ten patients with ordinary duodenal ulcer disease. Each of the tumor or pancreatic tissue extracts obtained from nine patients exhibited secretagogue activity in rats even though tissue gastrin content was 101.9 pmol (213.8 ng).g-1 or less. The secretagogue activity of the tumor extracts was confirmed in conscious gastric fistula dogs. The tumors' secretagogue activity, in contrast to gastrin, was destroyed by trypsin. It was eluted between porcine motilin and human gastrin I from a Sephadex G-50 (Pharmacia LKB Biotechnology, Inc., Piscataway, NJ) superfine column and was not retained by CM-cellulose, at pH 8.5. Its retention time during reverse phase HPLC on a C18 column also differed from those of G17 and G34. Thus, this secretagogue activity appeared mediated by a small, acidic peptide with a molecular size of about 2000 to 3000 daltons. The present study indicates that plasma and tumor extracts of these 11 patients contain a gastric acid secretagogue activity mediated by a nongastrin peptide. We suggest that what may be a distinct clinical entity associated with endocrine neoplasms of the pancreas should be considered in the face of excessive acid hypersecretion without fasting hypergastrinemia. PMID:2757418

Determination of the 4-monohydroxy metabolites of perhexiline in human plasma, urine and liver microsomes by liquid chromatography.

PubMed

Davies, Benjamin J; Herbert, Megan K; Coller, Janet K; Somogyi, Andrew A; Milne, Robert W; Sallustio, Benedetta C

2006-11-07

The use of perhexiline (PHX) is limited by hepatic and neurological toxicity associated with elevated concentrations in plasma that are the result of polymorphism of the cytochrome P450 2D6 isoform (CYP2D6). PHX is cleared by hepatic oxidation that produces three 4-monohydroxy metabolites: cis-OH-PHX, trans1-OH-PHX and trans2-OH-PHX. The current study describes an HPLC-fluorescent method utilising pre-column derivatization with dansyl chloride. Following derivatization, the metabolites were resolved on a C18 column with a gradient elution using a mobile phase composed of methanol and water. The method described is suitable for the quantification of the metabolites in human plasma and urine following clinical doses and for kinetic studies using human liver microsomes. The method demonstrates sufficient sensitivity, accuracy and precision between 5.0 and 0.01, 50.0 and 0.2 and 1.0 and 0.005 mg/l in human plasma, urine and liver microsomes, respectively, with intra-assay coefficients of variation and bias <15%, except at the lowest limit of quantification (<20%). The inter-assay coefficients of variation and bias were <15%. The application of this method to plasma and urine samples of five CYP2D6 extensive metaboliser (EM) patients at steady state with respect to PHX dosing determined that the mean (+/-S.D.) renal clearances of trans1-OH-PHX and cis-OH-PHX were 1.58+/-0.35 and 0.16+/-0.06l/h, respectively. The mean (+/-S.D.) dose recovered in urine as free and glucuronidated 4-monohydroxy PHX metabolites was 20.6+/-11.6%.

Initial design for an experimental investigation of strongly coupled plasma behavior in the Atlas facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Munson, C.P.; Benage, J.F. Jr.; Taylor, A.J.

Atlas is a high current ({approximately} 30 MA peak, with a current risetime {approximately} 4.5 {micro}sec), high energy (E{sub stored} = 24 MJ, E{sub load} = 3--6 MJ), pulsed power facility which is being constructed at Los Alamos National Laboratory with a scheduled completion date in the year 2000. When operational, this facility will provide a platform for experiments in high pressure shocks (> 20 Mbar), adiabatic compression ({rho}/{rho}{sub 0} > 5, P > 10 Mbar), high magnetic fields ({approximately} 2,000 T), high strain and strain rates ({var_epsilon} > 200%, d{var_epsilon}/dt {approximately} 10{sup 4} to 10{sup 6} s{sup {minus}1}), hydrodynamicmore » instabilities of materials in turbulent regimes, magnetized target fusion, equation of state, and strongly coupled plasmas. For the strongly coupled plasma experiments, an auxiliary capacitor bank will be used to generate a moderate density (< 0.1 solid), relatively cold ({approximately} 1 eV) plasma by ohmic heating of a conducting material of interest such as titanium. This stargate plasma will be compressed against a central column containing diagnostic instrumentation by a cylindrical conducting liner that is driven radially inward by current from the main Atlas capacitor bank. The plasma is predicted to reach densities of {approximately} 1.1 times solid, achieve ion and electron temperatures of {approximately} 10 eV, and pressures of {approximately} 4--5 Mbar. This is a density/temperature regime which is expected to experience strong coupling, but only partial degeneracy. X-ray radiography is planned for measurements of the material density at discrete times during the experiments; diamond Raman measurements are anticipated for determination of the pressure. In addition, a neutron resonance spectroscopic technique is being evaluated for possible determination of the temperature (through low percentage doping of the titanium with a suitable resonant material). Initial target plasma formation experiments are being planned on an existing pulsed power facility at LANL and will be completed before the start of operation of Atlas.« less

AWAKE readiness for the study of the seeded self-modulation of a 400 GeV proton bunch

NASA Astrophysics Data System (ADS)

Muggli, P.; Adli, E.; Apsimon, R.; Asmus, F.; Baartman, R.; Bachmann, A.-M.; Barros Marin, M.; Batsch, F.; Bauche, J.; Berglyd Olsen, V. K.; Bernardini, M.; Biskup, B.; Blanco Vinuela, E.; Boccardi, A.; Bogey, T.; Bohl, T.; Bracco, C.; Braunmuller, F.; Burger, S.; Burt, G.; Bustamante, S.; Buttenschön, B.; Butterworth, A.; Caldwell, A.; Cascella, M.; Chevallay, E.; Chung, M.; Damerau, H.; Deacon, L.; Dexter, A.; Dirksen, P.; Doebert, S.; Farmer, J.; Fedosseev, V.; Feniet, T.; Fior, G.; Fiorito, R.; Fonseca, R.; Friebel, F.; Gander, P.; Gessner, S.; Gorgisyan, I.; Gorn, A. A.; Grulke, O.; Gschwendtner, E.; Guerrero, A.; Hansen, J.; Hessler, C.; Hofle, W.; Holloway, J.; Hüther, M.; Ibison, M.; Islam, M. R.; Jensen, L.; Jolly, S.; Kasim, M.; Keeble, F.; Kim, S.-Y.; Kraus, F.; Lasheen, A.; Lefevre, T.; LeGodec, G.; Li, Y.; Liu, S.; Lopes, N.; Lotov, K. V.; Martyanov, M.; Mazzoni, S.; Medina Godoy, D.; Mete, O.; Minakov, V. A.; Mompo, R.; Moody, J.; Moreira, M. T.; Mitchell, J.; Mutin, C.; Norreys, P.; Öz, E.; Ozturk, E.; Pauw, W.; Pardons, A.; Pasquino, C.; Pepitone, K.; Petrenko, A.; Pitmann, S.; Plyushchev, G.; Pukhov, A.; Rieger, K.; Ruhl, H.; Schmidt, J.; Shalimova, I. A.; Shaposhnikova, E.; Sherwood, P.; Silva, L.; Sosedkin, A. P.; Speroni, R.; Spitsyn, R. I.; Szczurek, K.; Thomas, J.; Tuev, P. V.; Turner, M.; Verzilov, V.; Vieira, J.; Vincke, H.; Welsch, C. P.; Williamson, B.; Wing, M.; Xia, G.; Zhang, H.; AWAKE Collaboration

2018-01-01

AWAKE is a proton-driven plasma wakefield acceleration experiment. We show that the experimental setup briefly described here is ready for systematic study of the seeded self-modulation of the 400 GeV proton bunch in the 10 m long rubidium plasma with density adjustable from 1 to 10× {10}14 cm-3. We show that the short laser pulse used for ionization of the rubidium vapor propagates all the way along the column, suggesting full ionization of the vapor. We show that ionization occurs along the proton bunch, at the laser time and that the plasma that follows affects the proton bunch.

Helicon and Trivelpiece-Gould modes in uniform unbounded plasmas

NASA Astrophysics Data System (ADS)

Stenzel, R. L.; Urrutia, J. M.

2016-10-01

Helicon modes are whistler modes with angular orbital momentum caused by phase rotation in addition to the axial phase propagation. Although these modes have been associated with whistler eigenmodes in bounded plasma columns, they do exist in unbounded plasmas. Experiments in a large laboratory plasma show the wave excitation with phased antenna arrays, the wave field topology and the propagation of helicons. Low frequency whistlers can have two modes with different wavelengths at a given frequency, called helicons and Trivelpiece-Gould modes. The latter are whistler modes near the oblique cyclotron resonance. The oblique propagation is due to short radial wavelengths near the boundary. In unbounded plasmas, the oblique propagation arises from short azimuthal wavelengths. This has been observed in high-mode number helicons (e.g., m = 8). It creates wave absorption in the center of the helicon mode. The strong absorption of the wave can heat electrons and create perpendicular wave-particle interactions. These results may be of interest in space plasmas for scattering of energetic electrons and in helicon plasma sources for plasma processing and thruster applications. Work supported by NSF/DOE.

Apparatus and process for the separation of hydrophobic and hydrophilic particles using microbubble column flotation together with a process and apparatus for generation of microbubbles

DOEpatents

Yoon, R.H.; Adel, G.T.; Luttrell, G.H.

1992-12-01

A method and apparatus are disclosed for the microbubble flotation separation of very fine and coarse particles, especially coal and minerals, so as to produce high purity and high recovery efficiency. This is accomplished through the use of a flotation column, microbubbles, recycling of the flotation pulp, and countercurrent wash water to gently wash the froth. Also disclosed are unique processes and apparatus for generating microbubbles for flotation in a highly efficient and inexpensive manner using either a porous tube or in-line static generators. 14 figs.

Apparatus and process for the separation of hydrophobic and hydrophilic particles using microbubble column flotation together with a process and apparatus for generation of microbubbles

DOEpatents

Yoon, R.H.; Adel, G.T.; Luttrell, G.H.

1998-09-29

A method and apparatus are disclosed for the microbubble flotation separation of very fine and coarse particles, especially coal and minerals, so as to produce high purity and high recovery efficiency. This is accomplished through the use of a flotation column, microbubbles, recycling of the flotation pulp, and countercurrent wash water to gently wash the froth. Also disclosed are unique processes and apparatus for generating microbubbles for flotation in a highly efficient and inexpensive manner using either a porous tube or in-line static generators. 14 figs.

Virus elimination during the recycling of chromatographic columns used during the manufacture of coagulation factors.

PubMed

Roberts, Peter L

2014-07-01

Various chromatographic procedures are used during the purification and manufacture of plasma products such as coagulation factors. These steps contribute to the overall safety of such products by removing potential virus contamination. Virus removal by two affinity chromatography procedures, i.e. monoclonal antibody chromatography and metal chelate chromatography (immobilised metal ion affinity chromatography), used during the manufacture of the high purity factor VIII (Replenate®) and factor IX (Replenine®-VF), respectively, has been investigated. In addition, as these columns are recycled after use, the effectiveness of the sanitisation procedures for preventing possible cross-contamination, has also been investigated. Both chromatographic steps proved effective for eliminating a range of model enveloped and non-enveloped viruses by 4 to >6 and 5 to >8 log for the monoclonal and metal chelate columns, respectively. The effectiveness of the relatively mild column sanitisation conditions used, i.e. ethanol for factor IX and acetic acid for factor VIII, was confirmed using non-spiked column runs. The chemicals used contributed to virus elimination by inactivation and/or by physical removal of the virus. In summary, these studies demonstrate that potential virus contamination between chromatographic runs can be prevented when an effective column recycling and sanitisation procedure is included. Copyright © 2014 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

Method for liquid chromatographic extraction of strontium from acid solutions

DOEpatents

Horwitz, E. Philip; Dietz, Mark L.

1992-01-01

A method and apparatus for extracting strontium and technetium values from biological, industrial and environmental sample solutions using a chromatographic column is described. An extractant medium for the column is prepared by generating a solution of a diluent containing a Crown ether and dispersing the solution on a resin substrate material. The sample solution is highly acidic and is introduced directed to the chromatographic column and strontium or technetium is eluted using deionized water.

Analytical Capability of Plasma Spectrometry Team

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gallimore, David L.

2012-07-19

Samples analyzed were: (1) Pu and U metal; (2) Pu oxide for nuclear fuel; (3) {sup 238}Pu oxide for heat source; and (4) Nuclear forensic samples - filters, swipes. Sample preparations that we did were: metal dissolution, marple filter dissolution, Pu oxide closed vessel acid digestion, and column separation to remove Pu.

AN ACCELERATION MECHANISM FOR NEUTRON PRODUCTION IN Z-PINCH DISCHARGES,

DTIC Science & Technology

A model has been developed for the acceleration of deuterons in the tightly compressed column of a z-pinch discharge, in particular that of a plasma ... focus discharge. It was assumed that an annular current distribution undergoes a rapidly contracting transition to an axially peaked distribution, and

Quantitation of pilsicainide in microscale samples of human biological fluids using liquid chromatography-tandem mass spectrometry.

PubMed

Shimizu, Mikiko; Hashiguchi, Masayuki; Shiga, Tsuyoshi; Nakamura, Koichi; Tamura, Hiro-omi; Mochizuki, Mayumi

2015-03-15

This paper describes a sensitive, reliable method to determine pilsicainide (PLC) levels in microscale sample volumes of human biological fluids using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI). PLC and quinidine as an internal standard were extracted with diethylether from 0.1mL of alkalinized biological fluids. The extract was injected into an analytical column (l-column 2 ODS, 75mm×2.1mm i.d.). The mobile phase for separation consisted of 5mM ammonium acetate (pH 4.5)/methanol (4:1, v/v) and was delivered at a flow rate of 0.2mL/min. The drift voltage was 100V. The sampling aperture was heated at 120°C and the shield temperature was 260°C. The ion transitions used to monitor analytes were m/z 273→m/z 110 for PLC and m/z 325→m/z 79 for quinidine. The total time for chromatographic separation was less than 8min. The validated concentration ranges of this method for PLC were 5-2000ng/mL in plasma, 5-500ng/mL in ultrafiltered plasma solution, and 25-2000ng/mL in urine. Mean recoveries of PLC in plasma, ultrafiltered plasma solution, and urine were 93.2-99.7%, 91.4-100.6%, and 93.9-104.7%, respectively. Intra- and interday coefficients of variation for PLC were less than 6.0% and 4.3% in plasma, 6.1% and 3.7% in ultrafiltered plasma solution, and 5.4% and 2.5% in urine at the above concentration ranges, respectively. The lower limit of quantification for PLC in plasma, ultrafiltered plasma solution, and urine were 5ng/mL, 5ng/mL, and 25ng/mL, respectively. This method can be applied to pharmacokinetic study and therapeutic drug monitoring in special populations such as neonates, infants, and the elderly by making effective use of residual samples used for general clinical laboratory testing. Copyright © 2015 Elsevier B.V. All rights reserved.

What are the limits of energy focusing in sonoluminescence?

NASA Astrophysics Data System (ADS)

Putterman, Seth; Camara, C.; Kappus, B.; Su, C. K.; Kirilov, E.

2003-04-01

Sonoluminescence [SL] is amazing for the extraordinary degree by which ultrasonic energy can be focused by a cavitating bubble. Local energy dissipation exceeds Kirkhoff's law by 1E15 and the acoustic energy density concentrates by 12 orders of magnitude to create picosecond flashes of broadband ultraviolet light. At the minimum bubble radius, the acceleration exceeds 1E11 g and a megabar level shock wave is emitted into the surrounding fluid. For single bubbles driven at 30 KHz, SL is nature's smallest blackbody. This implies that the bubble's interior is such a dense plasma that the photon-matter mean free path is shorter than the wavelength of light, and suggests that SL originates in an unusual state of matter. Excitation of a vertical column of fluid [~10 Hz] so as to create a water hammer leads to the upscaling of SL and generation of flashes of light with 3E8 photons and peak powers approaching 1 W. At 1 MHz, the spectrum resembles bremsstrahlung from a transparent plasma with a temperature ~1 MK. At 10 MHz the collapsed size of the SL bubble approaches 10 nm, which raises the possibility that the SL parameter space may extend to the domain of quantum mechanics. [Research supported by DARPA and DOE.

Ion source development for a photoneutralization based NBI system for fusion reactors

NASA Astrophysics Data System (ADS)

Simonin, A.; de Esch, H. P. L.; Garibaldi, P.; Grand, C.; Bechu, S.; Bès, A.; Lacoste, A.

2015-04-01

The next step after ITER is to demonstrate the viability and generation of electricity by a future fusion reactor (DEMO). The specifications required to operate an NBI system on DEMO are very demanding. The system has to provide a very high level of power and energy, ~100MW of D° beam at 1MeV, including high wall-plug efficiency (η > 60%). For this purpose, a new injector concept, called Siphore, is under investigation between CEA and French universities. Siphore is based on the stripping of the accelerated negative ions by photo-detachment provided by several Fabry-Perot cavities (3.5MW of light power per cavity) implemented along the D- beam. The beamline is designed to be tall and narrow in order that the photon flux overlaps the entire negative ion beam. The paper will describe the present R&D at CEA which addresses the development of an ion source and pre-accelerator prototypes for Siphore, the main goal being to produce an intense negative ion beam sheet. The negative ion source Cybele is based on a magnetized plasma column where hot electrons are emitted from the source center. Parametric studies of the source are performed using Langmuir probes in order to characterize the plasma and to compare with numerical models being developed in French universities.

Buffalo plasma fibronectin: a physico-chemical study.

PubMed

Ahmed, N; Chandra, R; Raj, H G

2001-12-01

Plasma fibronectin (FN) of buffalo (Babulis babulis) was purified to apparent homogeneity, using gelatin-Sepharose and heparin-Sepharose affinity columns. It was found to have two subunits of molecular mass 246 kDa and 228 kDa, on SDS-gel. Its immunological cross-reactivity with anti-human plasma FN was confirmed by Western blotting. The amino acid composition was found to be similar to that of human and bovine plasma FNs. Buffalo plasma FN contained 2.23% neutral hexoses and 1.18% sialic acids. No titrable sulfhydryl group could be detected in the absence of denaturant. Reaction with DTNB indicated 3.4 sulfhydryl groups in the molecule, whereas BDC-OH titration gave a value of 3.8 -SH groups in buffalo plasma FN. Stoke's radius, intrinsic viscosity, diffusion coefficient and frictional ratio indicated that buffalo plasma FN did not have a compact globular conformation at physiological pH and ionic strength. Molecular dimensions (average length, 120 nm; molar mass to length ratio, 3950 nm(-1) and mean diameter, 2.4 nm) as revealed by rotary shadowing electron microscopy further supported the extended conformation of buffalo plasma FN. These results show that buffalo plasma FN has similar properties as that of human plasma FN.

Hydrogen sulfide generation in simulated construction and demolition debris landfills: impact of waste composition.

PubMed

Yang, Kenton; Xu, Qiyong; Townsend, Timothy G; Chadik, Paul; Bitton, Gabriel; Booth, Matthew

2006-08-01

Hydrogen sulfide (H2S) generation in construction and demolition (C&D) debris landfills has been associated with the biodegradation of gypsum drywall. Laboratory research was conducted to observe H2S generation when drywall was codisposed with different C&D debris constituents. Two experiments were conducted using simulated landfill columns. Experiment 1 consisted of various combinations of drywall, wood, and concrete to determine the impact of different waste constituents and combinations on H2S generation. Experiment 2 was designed to examine the effect of concrete on H2S generation and migration. The results indicate that decaying drywall, even alone, leached enough sulfate ions and organic matter for sulfate-reducing bacteria (SRB) to generate large H2S concentrations as high as 63,000 ppmv. The codisposed wastes show some effect on H2S generation. At the end of experiment 1, the wood/drywall and drywall alone columns possessed H2S concentrations > 40,000 ppmv. Conversely, H2S concentrations were < 1 ppmv in those columns containing concrete. Concrete plays a role in decreasing H2S by increasing pH out of the range for SRB growth and by reacting with H2S. This study also showed that wood lowered H2S concentrations initially by decreasing leachate pH values. Based on the results, two possible control mechanisms to mitigate H2S generation in C&D debris landfills are suggested.

Electrode assemblies, plasma generating apparatuses, and methods for generating plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kong, Peter C.; Grandy, Jon D.; Detering, Brent A.

Electrode assemblies for plasma reactors include a structure or device for constraining an arc endpoint to a selected area or region on an electrode. In some embodiments, the structure or device may comprise one or more insulating members covering a portion of an electrode. In additional embodiments, the structure or device may provide a magnetic field configured to control a location of an arc endpoint on the electrode. Plasma generating modules, apparatus, and systems include such electrode assemblies. Methods for generating a plasma include covering at least a portion of a surface of an electrode with an electrically insulating membermore » to constrain a location of an arc endpoint on the electrode. Additional methods for generating a plasma include generating a magnetic field to constrain a location of an arc endpoint on an electrode.« less

Designing plasmas for chronic wound disinfection

NASA Astrophysics Data System (ADS)

Nosenko, T.; Shimizu, T.; Morfill, G. E.

2009-11-01

Irradiation with low-temperature atmospheric-pressure plasma provides a promising method for chronic wound disinfection. To be efficient for this purpose, plasma should meet the following criteria: it should significantly reduce bacterial density in the wounded area, cause a long-term post-irradiation inhibition of bacterial growth, yet without causing any negative effect on human cells. In order to design plasmas that would satisfy these requirements, we assessed the relative contribution of different components with respect to bactericidal properties due to irradiation with argon plasma. We demonstrate that plasma-generated UV radiation is the main short-term sterilizing factor of argon plasma. On the other hand, plasma-generated reactive nitrogen species (RNS) and reactive oxygen species (ROS) cause a long-term 'after-irradiation' inhibition of bacterial growth and, therefore, are important for preventing wound recolonization with bacteria between two treatments. We also demonstrate that at certain concentrations plasma-generated RNS and ROS cause significant reduction of bacterial density, but have no adverse effect on human skin cells. Possible mechanisms of the different effects of plasma-generated reactive species on bacteria and human cells are discussed. The results of this study suggest that argon plasma for therapeutic purposes should be optimized in the direction of reducing the intensity of plasma-generated UV radiation and increasing the density of non-UV plasma products.

Laser Heating of Magnetically Confined Plasmas for X-Ray Production.

DTIC Science & Technology

1976-04-01

self atsrm r~ t ion factor (SAF) is an arbitrary program input whose inverse PF2’ IFCT = SA F ’ mul t ip l ies Equation (3.25 ) to set a black body limit...from He-like neon to severa l MW. A detailed hydrodynamic and atomic physics program developed for this project— .~ ~~~ DD ~~~~~~~ 1473 I H I ’ I D...Sciences North- west , Inc . (MSNW) program for DNA , laser heating of magnetically con- fined plasma columns was demonstrated and moderate Z gases (neon

JPRS Report, Science & Technology USSR: Physics & Mathematics.

DTIC Science & Technology

1991-06-06

through a fixed delay line ( Porro prism ) and then a polarizer, the transmitted one passed through an adjustable delay line ( Porro prism ) and then a...radiation emitted by a plasma liner upon its compression into a plasma column. The target is a layer of material forming a resonance -bound ion pair...4.8338 nm wavelength corresponding to the resonance line of the 2s2 2S1/2 —> 3p 2P1/2° transition in the lithium-like A1XI ion pumping the 2s2 ’S0

Application of high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for determination of chromium compounds in the air at the workplace.

PubMed

Stanislawska, Magdalena; Janasik, Beata; Wasowicz, Wojciech

2013-12-15

The toxicity and bioavailability of chromium species are highly dependable on the form or species, therefore determination of total chromium is insufficient for a complete toxicological evaluation and risk assessment. An analytical method for determination of soluble and insoluble Cr (III) and Cr (VI) compounds in welding fume at workplace air has been developed. The total chromium (Cr) was determined by using quadruple inductively coupled plasma mass spectrometry (ICP-MS) equipped with a dynamic reaction cell (DRC(®)). Soluble trivalent and hexavalent chromium compounds were determined by high performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). A high-speed, reversed-phase CR C8 column (PerkinElmer, Inc., Shelton, CT, USA) was used for the speciation of soluble Cr (III) and soluble Cr (VI). The separation was accomplished by interaction of the chromium species with the different components of the mobile phase. Cr (III) formed a complex with EDTA, i.e. retained on the column, while Cr (VI) existed in the solutions as dichromate. Alkaline extraction (2% KOH and 3% Na2CO3) and anion exchange column (PRP-X100, PEEK, Hamilton) were used for the separation of the total Cr (VI). The results of the determination of Cr (VI) were confirmed by the analysis of the certified reference material BCR CRM 545 (Cr (VI) in welding dust). The results obtained for the certified material (40.2±0.6 g kg(-1)) and the values recorded in the examined samples (40.7±0.6 g kg(-1)) were highly consistent. This analytical method was applied for the determination of chromium in the samples in the workplace air collected onto glass (Whatman, Ø 37 mm) and membrane filters (Sartorius, 0.8 μm, Ø 37 mm). High performance liquid chromatography with inductively coupled plasma mass spectrometry is a remarkably powerful and versatile technique for determination of chromium species in welding fume at workplace air. Crown Copyright © 2013 Published by Elsevier B.V. All rights reserved.

The perils of pathogen discovery: origin of a novel parvovirus-like hybrid genome traced to nucleic acid extraction spin columns.

PubMed

Naccache, Samia N; Greninger, Alexander L; Lee, Deanna; Coffey, Lark L; Phan, Tung; Rein-Weston, Annie; Aronsohn, Andrew; Hackett, John; Delwart, Eric L; Chiu, Charles Y

2013-11-01

Next-generation sequencing was used for discovery and de novo assembly of a novel, highly divergent DNA virus at the interface between the Parvoviridae and Circoviridae. The virus, provisionally named parvovirus-like hybrid virus (PHV), is nearly identical by sequence to another DNA virus, NIH-CQV, previously detected in Chinese patients with seronegative (non-A-E) hepatitis. Although we initially detected PHV in a wide range of clinical samples, with all strains sharing ∼99% nucleotide and amino acid identity with each other and with NIH-CQV, the exact origin of the virus was eventually traced to contaminated silica-binding spin columns used for nucleic acid extraction. Definitive confirmation of the origin of PHV, and presumably NIH-CQV, was obtained by in-depth analyses of water eluted through contaminated spin columns. Analysis of environmental metagenome libraries detected PHV sequences in coastal marine waters of North America, suggesting that a potential association between PHV and diatoms (algae) that generate the silica matrix used in the spin columns may have resulted in inadvertent viral contamination during manufacture. The confirmation of PHV/NIH-CQV as laboratory reagent contaminants and not bona fide infectious agents of humans underscores the rigorous approach needed to establish the validity of new viral genomes discovered by next-generation sequencing.

Reflection and backscattering of microwaves under doubling of the plasma density and displacement of the gyroresonance region during electron cyclotron resonance heating of plasma in the l-2M stellarator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Batanov, G. M.; Borzosekov, V. D.; Vasilkov, D. G.

Reflection and backscattering of high-power (400 kW) gyrotron radiation creating and heating plasma at the second harmonic of the electronic cyclotron frequency in the L-2M stellarator have been investigated experimentally. The effect of the displacement of the gyroresonance region from the axis of the plasma column under doubling of the plasma density on the processes of reflection and backscattering of microwave radiation has been examined. A near doubling of short-wavelength (k{sub ⊥} ≈ 30 cm{sup –1}) turbulent density fluctuations squared is observed. The change in the energy confinement time under variations of plasma parameters and characteristics of short-wavelength turbulence ismore » discussed. A discrepancy between the measured values of the reflection coefficient from the electron cyclotron resonance heating region and predictions of the one-dimensional model is revealed.« less

Numerical study on xenon positive column discharges of mercury-free lamp

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ouyang, Jiting; He, Feng; Miao, Jinsong

2007-02-15

In this paper, the numerical study has been performed on the xenon positive column discharges of mercury-free fluorescent lamp. The plasma discharge characteristics are analyzed by numerical simulation based on two-dimensional fluid model. The effects of cell geometry, such as the dielectric layer, the electrode width, the electrode gap, and the cell height, and the filling gas including the pressure and the xenon percentage are investigated in terms of discharge current and discharge efficiency. The results show that a long transient positive column will form in the xenon lamp when applying ac sinusoidal power and the lamp can operate inmore » a large range of voltage and frequency. The front dielectric layer of the cell plays an important role in the xenon lamp while the back layer has little effect. The ratio of electrode gap to cell height should be large to achieve a long positive column xenon lamp and higher efficiency. Increase of pressure or xenon concentration results in an increase of discharge efficiency and voltage. The discussions will be helpful for the design of commercial xenon lamp cells.« less

Hybridation of different chiral separation techniques with ICP-MS detection for the separation and determination of selenomethionine enantiomers: chiral speciation of selenized yeast.

PubMed

Méndez, S P; González, E B; Sanz-Medel, A

2001-05-01

Enantioseparation and determination of selenomethionine enantiomers in selenized yeast was investigated using chiral separation techniques based on different principles, coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for selenium-specific detection. High performance liquid chromatography (HPLC) on a beta-cyclodestrin (beta-CD) column, cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC), gas chromatography (GC) on a Chirasil-L-Val column, and HPLC on a Chirobiotic T column have been investigated as the chiral separation techniques. For HPLC separation on the beta-CD column, and also for CD-MEKC, selenomethionine enantiomers were derivatized with NDA/CN(-). For chiral separation by GC, selenomethionine enantiomers were converted into their N-trifluoroacetyl (TFA)-O-alkyl esters. The developed hybridation methodologies are compared with respect to enantioselectivity, sensitivity and analysis time. The usefulness of the best-suited method [HPLC (Chirobiotic T)-ICP-MS] was demonstrated by its application to the successful chiral speciation of selenium and D-and L-selenomethionine content determination in selenized yeast. Copyright 2001 John Wiley & Sons, Ltd.

Aqueous Plasma Pharmacy: Preparation Methods, Chemistry, and Therapeutic Applications

PubMed Central

Joslin, Jessica M.; McCall, James R.; Bzdek, Justin P.; Johnson, Derek C.; Hybertson, Brooks M.

2017-01-01

Plasma pharmacy is a subset of the broader field of plasma medicine. Although not strictly defined, the term aqueous plasma pharmacy (APP) is used to refer to the generation and distribution of reactive plasma-generated species in an aqueous solution followed by subsequent administration for therapeutic benefits. APP attempts to harness the therapeutic effects of plasma-generated oxidant species within aqueous solution in various applications, such as disinfectant solutions, cell proliferation related to wound healing, and cancer treatment. The subsequent use of plasma-generated solutions in the APP approach facilitates the delivery of reactive plasma species to internal locations within the body. Although significant efforts in the field of plasma medicine have concentrated on employing direct plasma plume exposure to cells or tissues, here we focus specifically on plasma discharge in aqueous solution to render the solution biologically active for subsequent application. Methods of plasma discharge in solution are reviewed, along with aqueous plasma chemistry and the applications for APP. The future of the field also is discussed regarding necessary research efforts that will enable commercialization for clinical deployment. PMID:28428835

Carbon X-ray absorption in the local ISM: fingerprints in X-ray Novae spectra

NASA Astrophysics Data System (ADS)

Gatuzz, Efraín; Ness, J.-U.; Gorczyca, T. W.; Hasoglu, M. F.; Kallman, Timothy R.; García, Javier A.

2018-06-01

We present a study of the C K-edge using high-resolution LETGS Chandra spectra of four novae during their super-soft-source (SSS) phase. We identified absorption lines due to C II Kα, C III Kα and C III Kβ resonances. We used these astronomical observations to perform a benchmarking of the atomic data, which involves wavelength shifts of the resonances and photoionization cross-sections. We used improved atomic data to estimate the C II and C III column densities. The absence of physical shifts for the absorption lines, the consistence of the column densities between multiple observations and the high temperature required for the SSS nova atmosphere modeling support our conclusion about an ISM origin of the respective absorption lines. Assuming a collisional ionization equilibrium plasma the maximum temperature derived from the ratio of C II/C III column densities of the absorbers correspond to Tmax < 3.05 × 104 K.

Enhancement of SPES source performances.

PubMed

Fagotti, E; Palmieri, A; Ren, X

2008-02-01

Installation of SPES source at LNL was finished in July 2006 and the first beam was extracted in September 2006. Commissioning results confirmed very good performance of the extracted current density. Conversely, source reliability was very poor due to glow-discharge phenomena, which were caused by the ion source axial magnetic field protruding in the high-voltage column. This problem was fixed by changing the stainless steel plasma electrode support with a ferromagnetic one. This new configuration required us to recalculate ion source solenoids positions and fields in order to recover the correct resonance pattern. Details on magnetic simulations and experimental results of high voltage column shielding are presented.

Non-modal analysis of the diocotron instability for cylindrical geometry with conducting boundary

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mikhailenko, V. V.; Seok Kim, Jin; Jo, Younghyun

2014-05-15

The temporal evolution of the linear diocotron instability of a cylindrical annular plasma column surrounded by a conducting boundary has been investigated by using the methodology of the cylindrical shearing modes. The linear solution of the initial and boundary-value problems is obtained which is valid for any time at which linear effects dominate. The solution reveals that the initial perturbations of the electron density pass through the stage of the non-modal evolution when the perturbation experiences spatio-temporal distortion pertinent to the considered geometry of the electron column. The result is confirmed by a two-dimensional cylindrical particle-in-cell simulation.

Low- and high-order harmonic generation in the extended plasmas produced by laser ablation of zinc and manganese targets

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ganeev, R. A., E-mail: rashid-ganeev@mail.ru; Physical Department, Voronezh State University, Voronezh 394006; Baba, M.

The systematic studies of the harmonic generation of ultrashort laser pulses in the 5-mm-long Zn and Mn plasmas (i.e., application of nanosecond, picosecond, and femtosecond pulses for ablation, comparison of harmonic generation from atomic, ionic, and cluster-contained species of plasma, variation of plasma length, two-color pump of plasmas, etc.) are presented. The conversion efficiency of the 11th–19th harmonics generated in the Zn plasma was ∼5 × 10{sup −5}. The role of the ionic resonances of Zn near the 9th and 10th harmonics on the enhancement of harmonics is discussed. The enhancement of harmonics was also analyzed using the two-color pump of extendedmore » plasmas, which showed similar intensities of the odd and even harmonics along the whole range of generation. The harmonics up to the 107th order were demonstrated in the case of manganese plasma. The comparison of harmonic generation in the 5-mm-long and commonly used short (≤0.5 mm) plasma plumes showed the advanced properties of extended media.« less

Solid expellant plasma generator

NASA Technical Reports Server (NTRS)

Stone, Nobie H. (Inventor); Poe, Garrett D. (Inventor); Rood, Robert (Inventor)

2010-01-01

An improved solid expellant plasma generator has been developed. The plasma generator includes a support housing, an electrode rod located in the central portion of the housing, and a mass of solid expellant material that surrounds the electrode rod within the support housing. The electrode rod and the solid expellant material are made of separate materials that are selected so that the electrode and the solid expellant material decompose at the same rate when the plasma generator is ignited. This maintains a point of discharge of the plasma at the interface between the electrode and the solid expellant material.

Prion removal effect of a specific affinity ligand introduced into the manufacturing process of the pharmaceutical quality solvent/detergent (S/D)-treated plasma OctaplasLG.

PubMed

Neisser-Svae, A; Bailey, A; Gregori, L; Heger, A; Jordan, S; Behizad, M; Reichl, H; Römisch, J; Svae, T-E

2009-10-01

A new chromatographic step for the selective binding of abnormal prion protein (PrP(Sc)) was developed, and optimization for PrP(Sc) capture was achieved by binding to an affinity ligand attached to synthetic resin particles. This step was implemented into the manufacturing process of the solvent/detergent (S/D)-treated biopharmaceutical quality plasma Octaplas to further improve the safety margin in terms of risk for variant Creutzfeldt-Jakob disease (vCJD) transmission. Intermediates and Octaplas final container material, spiked with hamster brain-derived PrP(Sc)-containing fractions, were used for experiments to establish the feasibility of introducing this novel chromatography step. The binding capacity per millilitre of ligand gel was determined under the selected manufacturing conditions. In addition, the specificity of the ligand gel to bind PrP(Sc) from human sources was investigated. A validated Western blot test was used for the identification and quantification of PrP(Sc). A reduction factor of > or = 3.0 log(10) could be demonstrated by Western blotting, utilizing the relevant Octaplas matrix from manufacturing. In this particular cell-free plasma solution, the PrP(Sc) binding capacity of the selected gel was very high (> or = 6 log(10) ID(50)/ml, equivalent to roughly 10 log(10) ID(50)/column at manufacturing scale). The gel binds specifically PrP(Sc) from both animal (hamster and mouse) and human (sporadic and variant CJD) sources. This new single-use, disposable PrP(Sc)-harvesting gel ensures a very high capacity in terms of removing the pathogenic agent causing vCJD from the new generation OctaplasLG, in the event that prions can be found in plasma from donors incubating the disease and thereby contaminating the raw material plasma used for manufacturing.

Cold Atmosphere Plasma in Cancer Therapy

NASA Astrophysics Data System (ADS)

Keidar, Michael

2012-10-01

Plasma is an ionized gas that is typically generated in high-temperature laboratory conditions. Recent progress in atmospheric plasmas led to the creation of cold plasmas with ion temperature close to room temperature. Areas of potential application of cold atmospheric plasmas (CAP) include dentistry, drug delivery, dermatology, cosmetics, wound healing, cellular modifications, and cancer treatment. Various diagnostic tools have been developed for characterization of CAP including intensified charge-coupled device cameras, optical emission spectroscopy and electrical measurements of the discharge propertied. Recently a new method for temporally resolved measurements of absolute values of plasma density in the plasma column of small-size atmospheric plasma jet utilizing Rayleigh microwave scattering was proposed [1,2]. In this talk we overview state of the art of CAP diagnostics and understanding of the mechanism of plasma action of biological objects. The efficacy of cold plasma in a pre-clinical model of various cancer types (long, bladder, and skin) was recently demonstrated [3]. Both in-vitro and in-vivo studies revealed that cold plasmas selectively kill cancer cells. We showed that: (a) cold plasma application selectively eradicates cancer cells in vitro without damaging normal cells. For instance a strong selective effect was observed; the resulting 60--70% of lung cancer cells were detached from the plate in the zone treated with plasma, whereas no detachment was observed in the treated zone for the normal lung cells under the same treatment conditions. (b) Significantly reduced tumor size in vivo. Cold plasma treatment led to tumor ablation with neighbouring tumors unaffected. These experiments were performed on more than 10 mice with the same outcome. We found that tumors of about 5mm in diameter were ablated after 2 min of single time plasma treatment. The two best known cold plasma effects, plasma-induced apoptosis and the decrease of cell migration velocity can have important implications in cancer treatment by localizing the affected area of the tissue and by decreasing metastasic development. In addition, cold plasma treatment has affected the cell cycle of cancer cells. In particular, cold plasma induces a 2-fold increase in cells at the G2/M-checkpoint in both papilloma and carcinoma cells at about 24 hours after treatment, while normal epithelial cells (WTK) did not show significant differences. It was shown that reactive oxygen species metabolism and oxidative stress responsive genes are deregulated. We investigated the production of reactive oxygen species (ROS) with cold plasma treatment as a potential mechanism for the tumor ablation observed. [4pt] [1] Shashurin A., Shneider M.N., Dogariu A., Miles R.B. and Keidar M. Appl. Phys. Lett. (2010) 96, 171502.[0pt] [2] Shashurin A., Shneider M.N., Keidar M. Plasma Sources Sci. Technol. 21 (2012) 034006.[0pt] [3]. M. Keidar, R. Walk, A. Shashurin, P. Srinivasan, A. Sandler, S. Dasgupta , R. Ravi, R. Guerrero-Preston, B. Trink, British Journal of Cancer, 105, 1295-1301, 2011

Use of the Oak Ridge National Laboratory tungsten-188/rhenium-188 generator for preparation of the rhenium-188 HDD/lipiodol complex for trans-arterial liver cancer therapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jeong, J M; Knapp Jr, Russ F

2008-01-01

This work describes the installation, use, and quality control (QC) of the alumina-based tungsten-188 ({sup 188}W)/rhenium-188 ({sup 188}Re) generators provided by the Oak Ridge National Laboratory (ORNL). In addition, methods used for concentration of the {sup 188}Re-perrhenate bolus and preparation of {sup 188}Re-labeled HDD (4-hexadecyl-2,2,9,9-tetramethyl-4,7-diaza-1,10-decanethiol) for trans-arterial administration for therapy of nonresectable liver cancer also are described. The {sup 188}W/{sup 188}Re generator has a long useful shelf-life of several months and is a convenient on-site {sup 188}Re production system. {sup 188}Re has excellent therapeutic and imaging properties (T{sub 1/2} 16.9 hours; E{sub {beta}max} 2.12 MeV; 155-keV gamma ray, 15%) andmore » is cost effectively obtained on demand by saline elution of the generator. The clinical efficacy of a variety of {sup 188}Re-labeled agents has been demonstrated for several therapeutic applications. Because of the favorable physical properties of {sup 188}Re, several {sup 188}Re-labeled agents are being developed and evaluated for the treatment of nonresectable/refractory liver cancer. {sup 188}Re-labeled HDD has been the most widely studied of these agents for this application and has been introduced into clinical trials at a number of institutions. The trans-arterial administration of {sup 188}Re-labeled agents for treatment of inoperable liver cancer requires use of high-level (1-2 Ci) {sup 188}W/{sup 188}Re generators. The handling of such high levels of {sup 188}Re imposes radiological precautions normally not encountered in a radiopharmacy and adequate care and ALARA (i.e., 'As Low As Reasonably Achievable') principles must be followed. The ORNL generator provides consistently high {sup 188}Re yields (>75%) and low {sup 188}W parent breakthrough (<10{sup -3}%) over an extended shelf-life of several months. However, the high elution volumes (20-40 mL for 1-2 Ci generators) can require concentration of the {sup 188}Re bolus by postelution passage through silver cation chloride trapping columns used in the cost-effective tandem cation/anion column system. The silver column removes the high levels of chloride anion as insoluble AgCl, thus allowing subsequent specific trapping of the perrhenate anion on the small (QMA SeaPak) anion column. This method permits subsequent elution of {sup 188}Re-perrhenate with a small volume of saline, providing a very high activity-concentration solution. Because the {sup 188}Re-specific volume-activity concentration continually decreases with time, the tandem system is especially effective method for extending the useful generator shelf-life. Low elution flow rates (<1 mL/min) minimize any high back pressure which may be encountered during generator/tandem column elution when using tightly packed, small-particle-size commercial columns. In-house preparation of silver cation columns is recommended since the chloride trapping capacity is essentially unlimited, it is inexpensive and not limited in availability to any one supplier, and back pressure can be eliminated by the use of larger particles. Methods for the preparation of {sup 188}Re-HDD have been optimized and this agent can be obtained in high yield (80%).« less

Use of the ORNL Tungsten-188/Rhenium-188 Generator for Preparation of the Rhenium-188 HDD/Lipiodol Complex for Transarterial Liver Cancer Therapy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Knapp Jr, Russ F; Jeong, J M

2008-01-01

This work describes the installation, use, and quality control (QC) of the alumina-based tungsten-188 ({sup 188}W)/rhenium-188 ({sup 188}Re) generators provided by the Oak Ridge National Laboratory (ORNL). In addition, methods used for concentration of the {sup 188}Re-perrhenate bolus and preparation of {sup 188}Re-labeled HDD (4-hexadecyl-2,2,9,9-tetramethyl-4,7-diaza-1,10-decanethiol) for trans-arterial administration for therapy of nonresectable liver cancer also are described. The {sup 188}W/{sup 188}Re generator has a long useful shelf-life of several months and is a convenient on-site {sup 188}Re production system. {sup 188}Re has excellent therapeutic and imaging properties (T{sub 1/2} 16.9 hours; E{beta}{sub max} 2.12 MeV; 155-keV gamma ray, 15%) andmore » is cost effectively obtained on demand by saline elution of the generator. The clinical efficacy of a variety of {sup 188}Re-labeled agents has been demonstrated for several therapeutic applications. Because of the favorable physical properties of {sup 188}Re, several {sup 188}Re-labeled agents are being developed and evaluated for the treatment of nonresectable/refractory liver cancer. {sup 188}Re-labeled HDD has been the most widely studied of these agents for this application and has been introduced into clinical trials at a number of institutions. The trans-arterial administration of {sup 188}Re-labeled agents for treatment of inoperable liver cancer requires use of high-level (1-2 Ci) {sup 188}W/{sup 188}Re generators. The handling of such high levels of {sup 188}Re imposes radiological precautions normally not encountered in a radiopharmacy and adequate care and ALARA (ie, 'As Low As Reasonably Achievable') principles must be followed. The ORNL generator provides consistently high {sup 188}Re yields (>75%) and low {sup 188}W parent breakthrough (<10{sup -3}%) over an extended shelf-life of several months. However, the high elution volumes (20-40 mL for 1-2 Ci generators) can require concentration of the {sup 188}Re bolus by postelution passage through silver cation chloride trapping columns used in the cost-effective tandem cation/anion column system. The silver column removes the high levels of chloride anion as insoluble AgCl, thus allowing subsequent specific trapping of the perrhenate anion on the small (QMA SeaPak) anion column. This method permits subsequent elution of {sup 188}Re-perrhenate with a small volume of saline, providing a very high activity-concentration solution. Because the {sup 188}Re-specific volume-activity concentration continually decreases with time, the tandem system is especially effective method for extending the useful generator shelf-life. Low elution flow rates (<1 mL/min) minimize any high back pressure which may be encountered during generator/tandem column elution when using tightly packed, small-particle-size commercial columns. In-house preparation of silver cation columns is recommended since the chloride trapping capacity is essentially unlimited, it is inexpensive and not limited in availability to any one supplier, and back pressure can be eliminated by the use of larger particles. Methods for the preparation of {sup 188}Re-HDD have been optimized and this agent can be obtained in high yield (80%).« less

Leaching of heavy metals from E-waste in simulated landfill columns

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li Yadong; Richardson, Jay B.; Mark Bricka, R.

2009-07-15

In recent history the volume of electronic products purchased by consumers has dramatically escalated. As a result this has produced an ever-increasing electronic waste (E-waste) stream, which has generated concerns regarding the E-waste's potential for adversely impacting the environment. The leaching of toxic substances from obsolete personal computers (PCs) and cathode ray tubes (CRTs) of televisions and monitors, which are the most significant components in E-waste stream, was studied using landfill simulation in columns. Five columns were employed. One column served as a control which was filled with municipal solid waste (MSW), two columns were filled with a mixture ofmore » MSW and CRTs, and the other two were filled with MSW and computer components including printed wire boards, hard disc drives, floppy disc drives, CD/DVD drives, and power supply units. The leachate generated from the columns was monitored for toxic materials throughout the two-year duration of the study. Results indicate that lead (Pb) and various other heavy metals that were of environmental and health concern were not detected in the leachate from the simulators. When the samples of the solids were collected from underneath the E-waste in the columns and were analyzed, significant amount of Pb was detected. This indicates that Pb could readily leach from the E-waste, but was absorbed by the solids around the E-waste materials. While Pb was not observed in the leachate in this study, it is likely that the Pb would eventually enter the leachate after a long term transport.« less

The Effect of Column and Eluent Fluorination on the Retention and Separation of non-Fluorinated Amino Acids and Proteins by HPLC

PubMed Central

Joyner, Katherine; Wang, Weizhen; Yu, Yihua Bruce

2011-01-01

The effect of column and eluent fluorination on the retention and separation of non-fluorinated amino acids and proteins in HPLC is investigated. A side-by-side comparison of fluorocarbon column and eluents (F-column and F-eluents) with their hydrocarbon counterparts (H-column and H-eluents) in the separation of a group of 33 analytes, including 30 amino acids and 3 proteins, is conducted. The H-column and the F-column contain the n-C8H17 group and n-C8F17 group, respectively, in their stationary phases. The H-eluents include ethanol (EtOH) and isopropanol (ISP) while the F-eluents include trifluoroethanol (TFE) and hexafluorosopropanol (HFIP). The 2 columns and 4 eluents generated 8 (column, eluent) pairs that produce 264 retention time data points for the 33 analytes. A statistical analysis of the retention time data reveals that although the H-column is better than the F-column in analyte separation and H-eluents are better than F-eluents in analyte retention, the more critical factor is the proper pairing of column with eluent. Among the conditions explored in this project, optimal retention and separation is achieved when the fluorocarbon column is paired with ethanol, even though TFE is the most polar one among the 4 eluents. This result shows fluorocarbon columns have much potential in chromatographic analysis and separation of non-fluorinated amino acids and proteins. PMID:21318121

SO2 columns over China: Temporal and spatial variations using OMI and GOME-2 observations

NASA Astrophysics Data System (ADS)

Huanhuan, Yan; Liangfu, Chen; Lin, Su; Jinhua, Tao; Chao, Yu

2014-03-01

Enhancements of SO2 column amounts due to anthropogenic emission sources over China were shown in this paper by using OMI and GOME-2 observations. The temporal and spatial variations of SO2 columns over China were analyzed for the time period 2005-2010. Beijing and Chongqing showed a high concentration in the SO2 columns, attributable to the use of coal for power generation in China and the characteristic of terrain and meteorology. The reduction of SO2 columns over Beijing and surrounding provinces in 2008 was observed by OMI, which confirms the effectiveness of strict controls on pollutant emissions and motor vehicle traffic before and during 2008 Olympic and Paralympic Games. The SO2 columns over China from GOME-2 (0.2-0.5 DU) were lower than those from OMI (0.6-1 DU), but both showed a decrease in SO2 columns over northern China since 2008 (except an increase in OMI SO2 in 2010).

Tropical intercontinental optical measurement network of aerosol, precipitable water and total column ozone

NASA Technical Reports Server (NTRS)

Holben, B. N.; Tanre, D.; Reagan, J. A.; Eck, T. F.; Setzer, A.; Kaufman, Y. A.; Vermote, E.; Vassiliou, G. D.; Lavenu, F.

1992-01-01

A new generation of automatic sunphotometers is used to systematically monitor clear sky total column aerosol concentration and optical properties, precipitable water and total column ozone diurnally and annually in West Africa and South America. The instruments are designed to measure direct beam sun, solar aureole and sky radiances in nine narrow spectral bands from the UV to the near infrared on an hourly basis. The instrumentation and the algorithms required to reduce the data for subsequent analysis are described.

Initial evaluation and comparison of plasma damage to atomic layer carbon materials using conventional and low T{sub e} plasma sources

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jagtiani, Ashish V.; Miyazoe, Hiroyuki; Chang, Josephine

2016-01-15

The ability to achieve atomic layer precision is the utmost goal in the implementation of atomic layer etch technology. Carbon-based materials such as carbon nanotubes (CNTs) and graphene are single atomic layers of carbon with unique properties and, as such, represent the ultimate candidates to study the ability to process with atomic layer precision and assess impact of plasma damage to atomic layer materials. In this work, the authors use these materials to evaluate the atomic layer processing capabilities of electron beam generated plasmas. First, the authors evaluate damage to semiconducting CNTs when exposed to beam-generated plasmas and compare thesemore » results against the results using typical plasma used in semiconductor processing. The authors find that the beam generated plasma resulted in significantly lower current degradation in comparison to typical plasmas. Next, the authors evaluated the use of electron beam generated plasmas to process graphene-based devices by functionalizing graphene with fluorine, nitrogen, or oxygen to facilitate atomic layer deposition (ALD). The authors found that all adsorbed species resulted in successful ALD with varying impact on the transconductance of the graphene. Furthermore, the authors compare the ability of both beam generated plasma as well as a conventional low ion energy inductively coupled plasma (ICP) to remove silicon nitride (SiN) deposited on top of the graphene films. Our results indicate that, while both systems can remove SiN, an increase in the D/G ratio from 0.08 for unprocessed graphene to 0.22 to 0.26 for the beam generated plasma, while the ICP yielded values from 0.52 to 1.78. Generally, while some plasma-induced damage was seen for both plasma sources, a much wider process window as well as far less damage to CNTs and graphene was observed when using electron beam generated plasmas.« less

Edge plasma boundary layer generated by kink modes in tokamaks

NASA Astrophysics Data System (ADS)

Zakharov, Leonid E.

2011-06-01

This paper describes the structure of the electric current generated by external wall touching and free boundary kink modes at the plasma edge using the ideally conducting plasma model. Both kinds of modes generate δ-functional surface current at the plasma edge. Free boundary kink modes also perturb the core plasma current, which in the plasma edge compensates the difference between the δ-functional surface currents of free boundary and wall touching kink modes. In addition, the resolution of an apparent paradox with the pressure balance across the plasma boundary in the presence of the surface currents is provided.

[Effects of the adsorbent CAA for hemopurification on normal components of human plasma in removing methylene blue].

PubMed

Ma, Yu; Xia, Yun; Yang, Xiaolan; Yu, Ming'an

2003-06-01

Virus inactivation of plasma can be achieved by phototreatment with methylene blue (MB). Subsequently, elimination of MB may reduce the adverse effects of MB. This study examined the effects of adsorbing MB with the use of cross-linked agar bead entrapped attapulgite clay (CAA) on normal ingredients in MB-treated plasma units. The biomedical characteristics of CAA were assessed by determination of partial biochemical indexes, coagulation potency and some cationic concentration in a control sample and the MB-treated plasma eluted from CAA column. The biochemistry indexes or K+, Na+ in plasma were almost unaltered before and after CAA adsorption. In contrast, the concentrations of CA2+ and Mg2+ increased and the blood ammonium decreased obviously. The activated partial thromboplastin time (APTT) was prolonged from 42 s to 53 s, and prothrombin time (PT) from 13 s to 14 s. The result indicates that CAA as an adsorbent for hemopurification retains the most important characters of human plasma. CAA can be useful for the elimination of MB in MB-treated plasma and does not bring on harmful alteration in clinical significance.

Partially ionized plasmas including the third symposium on uranium plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krishnan, M.

1976-09-01

Separate abstracts are included for 28 papers on electrically generated plasmas, fission generated plasmas, nuclear pumped lasers, gaseous fuel reactor research, and applications. Five papers have been previously abstracted and included in ERA.

Quasi-static modeling of beam-plasma and laser-plasma interactions

NASA Astrophysics Data System (ADS)

Huang, Chengkun

Plasma wave wakefields excited by either laser or particle beams can sustain acceleration gradients three orders of magnitude larger than conventional RF accelerators. They are promising for accelerating particles in short distances for applications such as future high-energy colliders, and medical and industrial accelerators. In a Plasma Wakefield Accelerator (PWFA) or a Laser Wakefield Accelerator (LWFA), an intense particle or laser beam drives a plasma wave and generates a strong wakefield which has a phase velocity equal to the velocity of the driver. This wakefield can then be used to accelerate part of the drive beam or a separate trailing beam. The interaction between the plasma and the driver is highly nonlinear and therefore a particle description is required for computer modeling. A highly efficient, fully parallelized, fully relativistic, three-dimensional particle-in-cell code called QuickPIC for simulating plasma and laser wakefield acceleration has been developed. The model is based on the quasi-static or frozen field approximation, which assumes that the drive beam and/or the laser does not evolve during the time it takes for it to pass a plasma particle. The electromagnetic fields of the plasma wake and its associated index of refraction are then used to evolve the driver using very large time steps. This algorithm reduces the computational time by at least 2 to 3 orders of magnitude. Comparison between the new algorithm and a fully explicit model (OSIRIS) are presented. The agreement is excellent for problems of interest. Direction for future work is also discussed. QuickPIC has been used to study the "afterburner" concept. In this concept a fraction of an existing high-energy beam is separated out and used as a trailing beam with the goal that the trailing beam acquires at least twice the energy of the drive beam. Several critical issues such as the efficient transfer of energy and the stable propagation of both the drive and trailing beams in the plasma are investigated. We have simulated a 100 GeV and a 1 TeV plasma "afterburner" stages for electron beams and the results are presented. QuickPIC also has enabled us to develop a new theory for understanding the hosing instability of the drive and trailing beams. The new theory is based on a perturbation to the ion column boundary which includes relativistic effects, axial motion and the full electromagnetic character of the wake. The new theory is verified by comparing it to the simulation results. In the adiabatic long beam limit it recovers the result of previous work from fluid models.

On Entropy Generation and the Effect of Heat and Mass Transfer Coupling in a Distillation Process

NASA Astrophysics Data System (ADS)

Burgos-Madrigal, Paulina; Mendoza, Diego F.; López de Haro, Mariano

2018-01-01

The entropy production rates as obtained from the exergy analysis, entropy balance and the nonequilibrium thermodynamics approach are compared for two distillation columns. The first case is a depropanizer column involving a mixture of ethane, propane, n-butane and n-pentane. The other is a weighed sample of Mexican crude oil distilled with a pilot scale fractionating column. The composition, temperature and flow profiles, for a given duty and operating conditions in each column, are obtained with the Aspen Plus V8.4 software by using the RateFrac model with a rate-based nonequilibrium column. For the depropanizer column the highest entropy production rate is found in the central trays where most of the mass transfer occurs, while in the second column the highest values correspond to the first three stages (where the vapor mixture is in contact with the cold liquid reflux), and to the last three stages (where the highest temperatures take place). The importance of the explicit inclusion of thermal diffusion in these processes is evaluated. In the depropanizer column, the effect of the coupling between heat and mass transfer is found to be negligible, while for the fractionating column it becomes appreciable.

Quantification of aesculin in rabbit plasma and ocular tissues by high performance liquid chromatography using fluorescent detection: application to a pharmacokinetic study.

PubMed

Chen, QiuHong; Zeng, Ying; Kuang, JianChao; Li, Ye; Li, XiaoHui; Zheng, Yu; Hou, Hua; Hou, ShiXiang

2011-04-28

A simple and sensitive high performance liquid chromatography method with fluorescence detection (HPLC-FD) was described for the determination of aesculin (AL) at low concentrations in rabbit plasma and ocular tissues. After deproteinization by methanol using pazufloxacin mesilate (PM) as an internal standard (I.S.), supernatants were evaporated to dryness at 40°C under a gentle stream of nitrogen. The residue was reconstituted in mobile phase and a volume of 20μL was injected into the HPLC for analysis. Analytes were separated on an Ultimate XB-C18 column (250mm × 4.6mm i.d., 5μm particle size) and protected by a ODS guard column (10mm × 4.0mm i.d., 5μm particle size), using acetonitrile-0.1% triethylamine in water (adjusted to pH 3.0 using phosphoric acid) (12:88, v/v) as mobile phase with a flow rate of 1.0mL/min. The wavelengths of fluorescence detector (FD) were set at 344nm for excitation and 466nm for emission. The lower limit of quantitation (LOQ) for AL was 0.80ng/mL for plasma and vitreous body, 1.59ng/mL for aqueous humor, and 6.55ng/g for iris and 1.66ng/g for retina. The method was used in the study of AL concentrations in plasma and ocular tissues after topical administration of AL eye drops. Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.

Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods.

PubMed

Kostić, Nađa; Dotsikas, Yannis; Jović, Nebojša; Stevanović, Galina; Malenović, Anđelija; Medenica, Mirjana

2015-05-10

In this paper, novel LC-MS/MS methods for the determination of antiepileptic drug pregabalin in dried matrix spots (DMS) are presented. This attractive technique of sample collection in micro amount was utilized in the form of dried blood spots (DBS) and dried plasma spots (DPS). Following a pre-column derivatization procedure, using n-propyl chloroformate in the presence of n-propanol, and consecutive liquid-liquid extraction, derivatized pregabalin and its internal standard, 4-aminocyclohexanecarboxylic acid, were detected in positive ion mode by applying two SRM transitions per analyte. A YMC-Pack Octyl column (50mm×4.0mm, 3μm particle size) maintained at 30°C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550μL/min and total run time 2min. Established methods were fully validated over the concentration range of 0.200-20.0μg/mL for DBS and 0.400-40.0μg/mL for DPS, respectively, while specificity, accuracy, precision, recovery, matrix-effect, stability, dilution integrity and spot homogeneity were found within acceptance criteria. Validated methods were applied for the determination of pregabalin levels in dried blood and plasma samples obtained from patients with epilepsy, after per os administration of commercial capsules. Comparison of drug level in blood and plasma, as well as correction steps undertaken in order to overcome hematocrit issue, when analyzing DBS, are also given. Copyright © 2015 Elsevier B.V. All rights reserved.

High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma.

PubMed

Rakic, Anita; Miljkovic, Branislava; Pokrajac, Milena; Vucicevic, Katarina

2007-03-12

A selective, sensitive, and simple high-performance liquid chromatographic (HPLC) method was developed for the determination of moclobemide and its two major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma. Sample preparation (0.5 ml of plasma) involved solid-phase extraction (SPE) using Speedisk H(2)O-Philic DVB columns. Separations were performed on a Waters XTerra RP18 column (5 microm, 150 mm x 4.6 mm). The mobile phase consisted of 10 mM KH(2)PO(4) with 1% triethylamine (pH 3.9) and acetonitrile (83:17, v/v), and a flow-rate was 1.2 ml/min. The total run time was 13 min. UV detection was performed at 240 nm. Mean absolute recoveries were > or =90% and the limit of quantification (LOQ) for all analytes was 0.02 mg/l. Calibration curves were linear (r>0.995) over a wide range of the analyte concentrations in plasma; thus, the method is suitable for different clinical studies when large variations in the drug/metabolites concentrations are observed. During a 5-day assay validation procedure the accuracy and precision were tested and proven (relative errors (RE)< or =13%; intra-day coefficient of variation (CV)< or =7%; inter-day CV< or =13%). Many drugs frequently used in the target patient population were evaluated for potential interference in order method selectivity to be ensured. The assay has been used in a clinical pharmacokinetic study to assess steady-state pharmacokinetics of moclobemide and two metabolites in depressive patients on mono- and combined therapy.

On-line solid phase extraction coupled to capillary LC-ESI-MS for determination of fluoxetine in human blood plasma.

PubMed

Saber, Amr L

2009-04-15

An instrumental setup including on-line solid phased extraction coupled to capillary liquid chromatography-electrospray ionization-mass spectrometry (SPE-capLC-ESI-MS) has been constructed to improve the sensitivity for quantification of fluoxetine hydrochloride in human plasma. Prior to injection, 0.5 mL of plasma spiked with metronidazole (internal standard) was mixed with ammonium formate buffer for effective chloroform liquid-liquid extraction. The method was validated in the range 5-60 ng mL(-1) fluoxetine, yielding a correlation coefficient of 0.999 (r(2)). The within-assay and between-assay precisions were between (8.5 and 11%) and (6.6 and 7.5%), respectively. The method was used to determine the amount of fluoxetine in a healthy male 14 h after an intake of one capsule of the antidepressant and anorectic Flutin, which contains 20mg fluoxetine per each capsule. Fluoxetine was detected, and the concentration was calculated to 9.0 ng mL(-1) plasma. In the preliminary experiments, conventional LC-UV instrumentation was employed. However, it was found that employing a capillary column with an inner diameter of (0.3mm I.D. x 50 mm, Zorbax C(18)) increased the sensitivity by a factor of approximately 100, when injecting the same mass of analyte. Incorporating an easily automated C(18) reversed phase column switching system with SPE (1.0mm I.D. x 5.0mm, 5 microm) made it possible to inject up to 100 microL of solution, and the total analysis time was 5.5 min.

A novel ultra performance liquid chromatography-tandem mass spectrometry method for the determination of sucrose octasulfate in dog plasma.

PubMed

Ke, Yuyong; Li, Steve Lianghong; Chang, Linda Dongxia; Kapanadze, Theo

2015-01-26

A novel, specific and sensitive bioanalytical method has been developed for the determination of sucrose octasulfate (SOS) in dog plasma and urine using ion-pair reversed-phase ultraperformance liquid chromatography coupled with electrospray triple quadruple mass spectrometry (IPRP-UPLC ESI MS/MS). (13)C-labeled sucrose octasulfate-(13)C12 sodium salt is used as the internal standard. 200 μL of plasma or serum sample is extracted using weak anion exchange solid phase cartridge. In this method, a polar amide column is employed for the liquid chromatograph (LC) separation while the diethylamine and formic acid buffer is used as the ion-pairing reagent. The low limitation of quantitation of sucrose octasulfate is 0.20 ng on the column with a signal to noise ratio larger than 50. Parameters such as linearity, accuracy and precision have been validated in full compliance with the FDA guidelines for the bioanalytical method development and validation. A linear regression model fit the calibration curve very well with R>0.99. The bias and coefficient of variation of all levels of QCs are within the range of 15%. The selectivity, matrix effect and stabilities of analytes in solution and matrix have also been evaluated and the results met the acceptance criteria according to the guidelines. Based on these results, the method has qualified to analyze sucrose octasulfate in dog plasma for clinic research. This method has been applied to 1000 preclinical samples. Copyright © 2014 Elsevier B.V. All rights reserved.

Determination of triapine, a ribonucleotide reductase inhibitor, in human plasma by liquid chromatography tandem mass spectrometry.

PubMed

Feng, Ye; Kunos, Charles A; Xu, Yan

2015-09-01

Triapine is an inhibitor of ribonucleotide reductase (RNR). Studies have shown that triapine significantly decreases the activity of RNR and enhanced the radiation-mediated cytotoxicity in cervical and colon cancer. In this work, we have developed and validated a selective and sensitive LC-MS/MS method for the determination of triapine in human plasma. In this method, 2-[(3-fluoro-2-pyridinyl)methylene] hydrazinecarbothioamide (NSC 266749) was used as the internal standard (IS); plasma samples were prepared by deproteinization with acetonitrile; tripaine and the IS were separated on a Waters Xbridge Shield RP18 column (3.5 µm; 2.1 × 50 mm) using a mobile phase containing 25.0% methanol and 75.0% ammonium bicarbonate buffer (10.0 mM, pH 8.50; v/v); column eluate was monitored by positive turbo-ionspray tandem mass spectrometry; and quantitation of triapine was carried out in multiple-reaction-monitoring mode. The method developed had a linear calibration range of 0.250-50.0 ng/mL with correlation coefficient of 0.999 for triapine in human plasma. The IS-normalized recovery and the IS-normalized matrix factor of triapine were 101-104% and 0.89-1.05, respectively. The accuracy expressed as percentage error and precision expressed as coefficient of variation were ≤±6 and ≤8%, respectively. The validated LC-MS/MS method was applied to the measurement of triapine in patient samples from a phase I clinical trial. Copyright © 2015 John Wiley & Sons, Ltd.

Laser-pulse shape effects on magnetic field generation in underdense plasmas

NASA Astrophysics Data System (ADS)

Gopal, Krishna; Raja, Md. Ali; Gupta, Devki Nandan; Avinash, K.; Sharma, Suresh C.

2018-07-01

Laser pulse shape effect has been considered to estimate the self-generated magnetic field in laser-plasma interaction. A ponderomotive force based physical mechanism has been proposed to investigate the self-generated magnetic field for different spatial profiles of the laser pulse in inhomogeneous plasmas. The spatially inhomogeneous electric field of a laser pulse imparts a stronger ponderomotive force on plasma electrons. Thus, the stronger ponderomotive force associated with the asymmetric laser pulse generates a stronger magnetic field in comparison to the case of a symmetric laser pulse. Scaling laws for magnetic field strength with the laser and plasma parameters for different shape of the pulse have been suggested. Present study might be helpful to understand the plasma dynamics relevant to the particle trapping and injection in laser-plasma accelerators.

Model Predictive Control-based Power take-off Control of an Oscillating Water Column Wave Energy Conversion System

NASA Astrophysics Data System (ADS)

Rajapakse, G.; Jayasinghe, S. G.; Fleming, A.; Shahnia, F.

2017-07-01

Australia’s extended coastline asserts abundance of wave and tidal power. The predictability of these energy sources and their proximity to cities and towns make them more desirable. Several tidal current turbine and ocean wave energy conversion projects have already been planned in the coastline of southern Australia. Some of these projects use air turbine technology with air driven turbines to harvest the energy from an oscillating water column. This study focuses on the power take-off control of a single stage unidirectional oscillating water column air turbine generator system, and proposes a model predictive control-based speed controller for the generator-turbine assembly. The proposed method is verified with simulation results that show the efficacy of the controller in extracting power from the turbine while maintaining the speed at the desired level.

Variable Specific Impulse Magnetoplasma Rocket Engine

NASA Technical Reports Server (NTRS)

Chang-Diaz, Franklin R. (Inventor)

2002-01-01

An engine is disclosed, including a controllable output plasma generator, a controllable heater for selectably raising a temperature of the plasma connected to an outlet of the plasma generator, and a nozzle connected to an outlet of the heater, through which heated plasma is discharged to provide thrust. In one embodiment, the source of plasma is a helicon generator. In one embodiment, the heater is an ion cyclotron resonator. In one embodiment, the nozzle is a radially diverging magnetic field disposed on a discharge side of the heater so that helically travelling particles in the beater exit the heater at high axial velocity. A particular embodiment includes control circuits for selectably directing a portion of radio frequency power from an RF generator to the helicon generator and to the cyclotron resonator so that the thrust output and the specific impulse of the engine can be selectively controlled. A method of propelling a vehicle is also disclosed. The method includes generating a plasma, heating said plasma, and discharging the heated plasma through a nozzle. In one embodiment, the nozzle is a diverging magnetic field. In this embodiment, the heating is performed by applying a radio frequency electro magnetic field to the plasma at the ion cyclotron frequency in an axially polarized DC magnetic field.