Neural Parallel Engine: A toolbox for massively parallel neural signal processing.

PubMed

Tam, Wing-Kin; Yang, Zhi

2018-05-01

Large-scale neural recordings provide detailed information on neuronal activities and can help elicit the underlying neural mechanisms of the brain. However, the computational burden is also formidable when we try to process the huge data stream generated by such recordings. In this study, we report the development of Neural Parallel Engine (NPE), a toolbox for massively parallel neural signal processing on graphical processing units (GPUs). It offers a selection of the most commonly used routines in neural signal processing such as spike detection and spike sorting, including advanced algorithms such as exponential-component-power-component (EC-PC) spike detection and binary pursuit spike sorting. We also propose a new method for detecting peaks in parallel through a parallel compact operation. Our toolbox is able to offer a 5× to 110× speedup compared with its CPU counterparts depending on the algorithms. A user-friendly MATLAB interface is provided to allow easy integration of the toolbox into existing workflows. Previous efforts on GPU neural signal processing only focus on a few rudimentary algorithms, are not well-optimized and often do not provide a user-friendly programming interface to fit into existing workflows. There is a strong need for a comprehensive toolbox for massively parallel neural signal processing. A new toolbox for massively parallel neural signal processing has been created. It can offer significant speedup in processing signals from large-scale recordings up to thousands of channels. Copyright © 2018 Elsevier B.V. All rights reserved.

Flow cytometry for enrichment and titration in massively parallel DNA sequencing

PubMed Central

Sandberg, Julia; Ståhl, Patrik L.; Ahmadian, Afshin; Bjursell, Magnus K.; Lundeberg, Joakim

2009-01-01

Massively parallel DNA sequencing is revolutionizing genomics research throughout the life sciences. However, the reagent costs and labor requirements in current sequencing protocols are still substantial, although improvements are continuously being made. Here, we demonstrate an effective alternative to existing sample titration protocols for the Roche/454 system using Fluorescence Activated Cell Sorting (FACS) technology to determine the optimal DNA-to-bead ratio prior to large-scale sequencing. Our method, which eliminates the need for the costly pilot sequencing of samples during titration is capable of rapidly providing accurate DNA-to-bead ratios that are not biased by the quantification and sedimentation steps included in current protocols. Moreover, we demonstrate that FACS sorting can be readily used to highly enrich fractions of beads carrying template DNA, with near total elimination of empty beads and no downstream sacrifice of DNA sequencing quality. Automated enrichment by FACS is a simple approach to obtain pure samples for bead-based sequencing systems, and offers an efficient, low-cost alternative to current enrichment protocols. PMID:19304748

Ray tracing on the MPP

NASA Technical Reports Server (NTRS)

Dorband, John E.

1987-01-01

Generating graphics to faithfully represent information can be a computationally intensive task. A way of using the Massively Parallel Processor to generate images by ray tracing is presented. This technique uses sort computation, a method of performing generalized routing interspersed with computation on a single-instruction-multiple-data (SIMD) computer.

Too Much Information--Too Much Apprehension

ERIC Educational Resources Information Center

Hijazi, Sam

2004-01-01

The information age along with the exponential increase in information technology has brought an unexpected amount of information. The endeavor to sort and extract a meaning from the massive amount of data has become a challenging task to many educators and managers. This research is an attempt to collect the most common suggestions to reduce the…

How Massive Single Stars End Their Life

NASA Technical Reports Server (NTRS)

Heger, A.; Fryer, C. L.; Woosley, S. E.; Langer, N.; Hartmann, D. H.

2003-01-01

How massive stars die-what sort of explosion and remnant each produces-depends chiefly on the masses of their helium cores and hydrogen envelopes at death. For single stars, stellar winds are the only means of mass loss, and these are a function of the metallicity of the star. We discuss how metallicity, and a simplified prescription for its effect on mass loss, affects the evolution and final fate of massive stars. We map, as a function of mass and metallicity, where black holes and neutron stars are likely to form and where different types of supernovae are produced. Integrating over an initial mass function, we derive the relative populations as a function of metallicity. Provided that single stars rotate rapidly enough at death, we speculate on stellar populations that might produce gamma-ray bursts and jet-driven supernovae.

Heavy mineral sorting and distributions within massive sandstone divisions (Bouma A divisions) of Brushy Canyon Formation turbidites

NASA Astrophysics Data System (ADS)

Motanated, K.; Tice, M. M.

2009-12-01

KANNIPA MOTANATED and MICHAEL M. TICE Department of Geology and Geophysics, Texas A&M University, College Station, TX 77843-3115, USA Sediment sorting data are commonly used for interpreting depositional environments, analyzing mechanisms of deposition and transportation, and inferring relative transport distance of sediments. Typically, sorting in sandstones is estimated by point-counting thin sections which is a time consuming procedure and requires cutting sections of rock samples. We demonstrate a new technique for quantifying sediment sorting using element distribution maps obtained by x-ray fluorescence microscopy. We show that hydraulic sorting of Zr- and Ti- bearing grains (probably zircon and rutile, respectively) results in characteristic vertical profiles of Zr and Ti abundances within the Bouma A divisions of turbidites of the Brushy Canyon Formation, Delaware Basin, southern New Mexico. Zr- and Ti- bearing grains decrease in abundance and diameter from bases to tops of A divisions in every sample examined in this study. These results contrast with previous observations which suggest that grading in Brushy Canyon Formation structureless sandstones is absent or rare. The data support turbiditic interpretations of these rocks against traction current interpretations which rely on the lack of textural grading. Grading is reflected in vertical profiles of Ti/Al, Zr/Al and Zr/Ti ratios, which each decrease upward. These compositional variations could potentially be used as geochemical proxies for physical sorting, and might be useful for inferring depositional processes and relative transport distances.

Method and apparatus for analyzing error conditions in a massively parallel computer system by identifying anomalous nodes within a communicator set

DOEpatents

Gooding, Thomas Michael [Rochester, MN

2011-04-19

An analytical mechanism for a massively parallel computer system automatically analyzes data retrieved from the system, and identifies nodes which exhibit anomalous behavior in comparison to their immediate neighbors. Preferably, anomalous behavior is determined by comparing call-return stack tracebacks for each node, grouping like nodes together, and identifying neighboring nodes which do not themselves belong to the group. A node, not itself in the group, having a large number of neighbors in the group, is a likely locality of error. The analyzer preferably presents this information to the user by sorting the neighbors according to number of adjoining members of the group.

The Impact of Frontal and Non-Frontal Brain Tumor Lesions on Wisconsin Card Sorting Test Performance

ERIC Educational Resources Information Center

Goldstein, B.; Obrzut, J. E.; John, C.; Ledakis, G.; Armstrong, C. L.

2004-01-01

Several lesion and imaging studies have suggested that the Wisconsin Card Sorting Test (WCST) is a measure of executive dysfunction. However, some studies have reported that this measure has poor anatomical specificity because patients with either frontal or non-frontal focal lesions exhibit similar performance. This study examined 25 frontal, 20…

The Narrowing Gap in New York City Teacher Qualifications and Its Implications for Student Achievement in High-Poverty Schools

ERIC Educational Resources Information Center

Boyd, Donald; Lankford, Hamilton; Loeb, Susanna; Rockoff, Jonah; Wyckoff, James

2008-01-01

Understanding what makes an effective teacher, as well as how teachers sort by their effectiveness across schools, is central to understanding and addressing student achievement gaps. Prior studies have found substantial sorting of teachers across schools, with the schools with the highest proportions of poor, non-white, and low-scoring students…

a UV Spectral Library of Metal-Poor Massive Stars

NASA Astrophysics Data System (ADS)

Robert, Carmelle

1994-01-01

We propose to use the FOS to build a snapshot library of UV spectra of a sample of about 50 metal-poor massive stars located in the Magellanic Clouds. The majority of libraries already existing contains spectra of hot stars with chemical abundances close to solar. The high spectral resolution achieves with the FOS will be a major factor for the uniqueness of this new library. UV spectral libraries represent fundamental tools for the study of the massive star populations of young star-forming regions. Massive stars, which are impossible to identify directly in the optical-IR part of a composite spectrum, display on the other hand key signatures in the UV region. These signatures are mainly broad, metallicity dependent spectral features formed in the hot star winds. They require a high spectral resolution (of the order of 200-300 km/s) for an adequate study. A spectral library of metal-poor massive stars represents also a unique source of data for a stellar atmosphere analysis. Within less then 10 min we will obtain a high signal-to-noise ratio of at least 30. Finally, since short exposure times are possible, this proposal makes extremely good use of the capabilities of HST. We designed an observing strategy which yields a maximum scientific return at a minimum cost of spacecraft time.

Petrography, mineralogy, and geochemistry of deep gravelly sands in the Eyreville B core, Chesapeake Bay impact structure

USGS Publications Warehouse

Bartosova, Katerina; Gier, Susanne; Horton, J. Wright; Koeberl, Christian; Mader, Dieter; Dypvik, Henning

2010-01-01

The ICDP–USGS Eyreville drill cores in the Chesapeake Bay impact structure reached a total depth of 1766 m and comprise (from the bottom upwards) basement-derived schists and granites/pegmatites, impact breccias, mostly poorly lithified gravelly sand and crystalline blocks, a granitic slab, sedimentary breccias, and postimpact sediments. The gravelly sand and crystalline block section forms an approximately 26 m thick interval that includes an amphibolite block and boulders of cataclastic gneiss and suevite. Three gravelly sands (basal, middle, and upper) are distinguished within this interval. The gravelly sands are poorly sorted, clast supported, and generally massive, but crude size-sorting and subtle, discontinuous layers occur locally. Quartz and K-feldspar are the main sand-size minerals and smectite and kaolinite are the principal clay minerals. Other mineral grains occur only in accessory amounts and lithic clasts are sparse (only a few vol%). The gravelly sands are silica rich (~80 wt% SiO2). Trends with depth include a slight decrease in SiO2 and slight increase in Fe2O3. The basal gravelly sand (below the cataclasite boulder) has a lower SiO2 content, less K-feldspar, and more mica than the higher sands, and it contains more lithic clasts and melt particles that are probably reworked from the underlying suevite. The middle gravelly sand (below the amphibolite block) is finer-grained, contains more abundant clay minerals, and displays more variable chemical compositions than upper gravelly sand (above the block). Our mineralogical and geochemical results suggest that the gravelly sands are avalanche deposits derived probably from the nonmarine Potomac Formation in the lower part of the target sediment layer, in contrast to polymict diamictons higher in the core that have been interpreted as ocean-resurge debris flows, which is in agreement with previous interpretations. The mineralogy and geochemistry of the gravelly sands are typical for a passive continental margin source. There is no discernible mixing with marine sediments (no glauconite or Paleogene marine microfossils noted) during the impact remobilization and redeposition. The unshocked amphibolite block and cataclasite boulder might have originated from the outer parts of the transient crater.

Geomorphological map and preliminary analysis of Quaternary sediments in the Planica-Tamar valley (Julian Alps, NW Slovenia)

NASA Astrophysics Data System (ADS)

Novak, Andrej; Šmuc, Andrej

2016-04-01

The Planica-Tamar valley is located in the Julian Alps in north-west Slovenia. The Planica-Tamar valley represents typical mountain glacial valley bounded by steep, mainly carbonate cliffs with some glacial deposits still preserved. The valley is currently being filled with numerous Holocene sediments deposited by rock falls, landslides, mass gravity flows and fluvial flows. These deposits are forming active or inactive interfingering talus slopes, alluvial and debris-flow fans, all of them with a complex history of sedimentation and erosion forming unconformity bounded sedimentary units. In order to make a thorough analysis of these deposits a detailed geomorphological map in a scale of 1:10 000 has been made. Six different types of sedimentary deposits were defined and mapped. These are moraines, lacustrine sediments, fluvio-glacial deposits, talus slopes, debris fans and alluvial fans. Other mapped features also include shape of ravines, their depths, ridges and direction of sedimentary flow. Additionally areas of active, semi-active and inactive sedimentation were marked. Moraines forms a ridge in the bottom of the valleys and are composed of unconsolidated, poorly sorted, subangular grains ranging from clay size to a few cubic meters big blocks. Lacustrine sediments are represented by laminated well sorted sand and silt, while fluvio-glacial deposits are composed of washed out subrounded sands and gravels. Talus slope deposits are characterised by clast-supported poorly sorted very angular gravel. Debris flow fans are represented by extremely poorly sorted matrix-supported gravels with grain size ranging from clay to few cubic meters big blocks. Alluvial fans are composed by variety of sedimentary textures. Sediments at the fan apex are clast-supported poorly sorted very angular gravels with up to a few cubic meters big block. In the middle part of the fan the sieve deposits are common, while in the distal parts a few centimeters thick layers of sand and moderately sorted clast or sandy matrix-supported angular gravels occur. In cross-sections of alluvial fans distinct palaeosoil horizons are present indicating longer inactivity of that part of the fan. The geomorphological map forms a base for further research and thorough analysis of Quaternary deposits in order to reconstruct the Holocene dynamic of triggering and sedimentation of different types of slope deposits and relate them to base rock geology, tectonic and local/regional climate events. Key words: geomorphological mapping, Holocene slope deposits, alluvial fans, debris fans, Alpine geomorphology.

The genesis of the newly discovered giant Wuben magmatic Fe-Ti oxide deposit in the Emeishan Large Igneous Province: a product of the late-stage redistribution and sorting of crystal slurries

NASA Astrophysics Data System (ADS)

Bai, Zhong-Jie; Zhong, Hong; Zhu, Wei-Guang; Hu, Wen-Jun; Chen, Cai-Jie

2018-04-01

A giant Fe-Ti oxide deposit hosted by the Wuben mafic intrusion has recently been discovered in the Pan-Xi area of the Emeishan Large Igneous Province (ELIP). The evolved compositions of the gangue minerals within the Fe-Ti oxide ores indicate that they formed during later stages of magma differentiation than those within the neighboring Panzhihua intrusion or other ore-bearing intrusions in this area. The rocks from the Wuben intrusion and MZb of the Panzhihua intrusion contain compositionally similar silicate minerals and have similar titanomagnetite/ilmenite ratios, suggesting that the former is related to and probably connected to the latter by subsurface magmatic conduits. This indicates that unconsolidated minerals that formed in the MZb flowed as crystal slurries into the Wuben magma chamber during the later stages of evolution of the parental magma. The later secondary enrichment of Fe-Ti oxides by mechanical redistribution and the sorting of crystals as a result of density and size differences generated the Wuben massive Fe-Ti oxide bodies. The ilmenite was commonly saturated in the magma at late stage of differentiation in the ELIP, thereby the associated deposit contains much higher contents of ilmenite. This indicates that future exploration for Fe-Ti oxide mineralization in the ELIP should not merely focus on the lower parts of large layered intrusions but should also include nearby relatively small intrusions or even the upper parts of large intrusions, especially as ilmenite-enriched Fe-Ti oxide deposits may have greater economic value than ilmenite-poor deposits.

Sedimentary deposits study of the 2006 Java tsunami, in Pangandaran, West Java (preliminary result)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maemunah, Imun, E-mail: imun-m2001@yahoo.com; Institute Technology of Bandung; Suparka, Emmy, E-mail: emmy@gc.itb.ac.id

The 2006 Java Earthquake (Mw 7.2) has generated a tsunami that reached Pangandaran coastal plain with 9.7 m above sea level height of wave. In 2014 we examined the tsunami deposit exposed in shallow trenches along a∼300 m at 5 transect from shoreline to inland on Karapyak and Madasari, Pangandaran. We documented stratigraphically and sedimentologically, the characteristics of Java Tsunami deposit on Karapyak and Madasari and compared both sediments. In local farmland a moderately-sorted, brown soil is buried by a poorly-sorted, grey, medium-grained sand-sheet. The tsunami deposit was distinguished from the underlying soil by a pronounced increase in grain size that becomesmore » finner upwards and landwards. Decreasing concentration of coarse size particles with distance toward inland are in agreement with grain size analysis. The thickest tsunami deposit is about 25 cm found at 84 m from shoreline in Madasari and about 15 cm found at 80 m from shoreline in Karapyak. The thickness of tsunami deposits in some transect become thinner landward but in some other transect lack a consistent suggested strongly affected by local topography. Tsunami deposits at Karapyak and Madasari show many similarities. Both deposits consist of coarse sand that sharply overlies a finer sandy soil. The presence mud drapes and other sedimentary structure like graded bedding, massive beds, mud clasts in many locations shows a dynamics process of tsunami waves. The imbrication coarse and shell fragments of the 2006 Java, tsunami deposits also provide information about the curent direction, allowing us to distinguish run up deposits from backwash deposits.« less

Nucleosynthesis in the first massive stars

NASA Astrophysics Data System (ADS)

Choplin, Arthur; Meynet, Georges; Maeder, André; Hirschi, Raphael; Chiappini, Cristina

2018-01-01

The nucleosynthesis in the first massive stars may be constrained by observing the surface composition of long-lived very iron-poor stars born around 10 billion years ago from material enriched by their ejecta. Many interesting clues on physical processes having occurred in the first stars can be obtained based on nuclear aspects. First, in these first massive stars, mixing must have occurred between the H-burning and the He-burning zone during their nuclear lifetimes; Second, only the outer layers of these massive stars have enriched the material from which the very iron-poor stars, observed today in the halo of the MilkyWay, have formed. These two basic requirements can be obtained by rotating stellar models at very low metallicity. In the present paper, we discuss the arguments supporting this view and illustrate the sensitivity of the results concerning the [Mg/Al] ratio on the rate of the reaction 23Na(p,γ)24Mg.

Imprints of fast-rotating massive stars in the Galactic Bulge.

PubMed

Chiappini, Cristina; Frischknecht, Urs; Meynet, Georges; Hirschi, Raphael; Barbuy, Beatriz; Pignatari, Marco; Decressin, Thibaut; Maeder, André

2011-04-28

The first stars that formed after the Big Bang were probably massive, and they provided the Universe with the first elements heavier than helium ('metals'), which were incorporated into low-mass stars that have survived to the present. Eight stars in the oldest globular cluster in the Galaxy, NGC 6522, were found to have surface abundances consistent with the gas from which they formed being enriched by massive stars (that is, with higher α-element/Fe and Eu/Fe ratios than those of the Sun). However, the same stars have anomalously high abundances of Ba and La with respect to Fe, which usually arises through nucleosynthesis in low-mass stars (via the slow-neutron-capture process, or s-process). Recent theory suggests that metal-poor fast-rotating massive stars are able to boost the s-process yields by up to four orders of magnitude, which might provide a solution to this contradiction. Here we report a reanalysis of the earlier spectra, which reveals that Y and Sr are also overabundant with respect to Fe, showing a large scatter similar to that observed in extremely metal-poor stars, whereas C abundances are not enhanced. This pattern is best explained as originating in metal-poor fast-rotating massive stars, which might point to a common property of the first stellar generations and even of the 'first stars'.

Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.

PubMed

Cioni, Jean-Michel; Wong, Hovy Ho-Wai; Bressan, Dario; Kodama, Lay; Harris, William A; Holt, Christine E

2018-03-07

The axons of retinal ganglion cells (RGCs) are topographically sorted before they arrive at the optic tectum. This pre-target sorting, typical of axon tracts throughout the brain, is poorly understood. Here, we show that cytoplasmic FMR1-interacting proteins (CYFIPs) fulfill non-redundant functions in RGCs, with CYFIP1 mediating axon growth and CYFIP2 specifically involved in axon sorting. We find that CYFIP2 mediates homotypic and heterotypic contact-triggered fasciculation and repulsion responses between dorsal and ventral axons. CYFIP2 associates with transporting ribonucleoprotein particles in axons and regulates translation. Axon-axon contact stimulates CYFIP2 to move into growth cones where it joins the actin nucleating WAVE regulatory complex (WRC) in the periphery and regulates actin remodeling and filopodial dynamics. CYFIP2's function in axon sorting is mediated by its binding to the WRC but not its translational regulation. Together, these findings uncover CYFIP2 as a key regulatory link between axon-axon interactions, filopodial dynamics, and optic tract sorting. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

Drainage evolution in the debris avalanche deposits near Mount Saint Helens, Washington

NASA Technical Reports Server (NTRS)

Beach, G. L.; Dzurisin, D.

1984-01-01

The 18 May 1980 eruption of Mount St. Helens was initiated by a massive rockslide-debris avalanche which completely transformed the upper 25 km of the North Fork Toutle River valley. The debris was generated by one of the largest gravitational mass movements ever recorded on Earth. Moving at an average velocity of 35 m/s, the debris avalanche buried approximately 60 sq km of terrain to an average depth of 45 m with unconsolidated, poorly sorted volcaniclastic material, all within a period of 10 minutes. Where exposed and unaltered by subsequent lahars and pyroclastic flows, the new terrain surface was characterized predominantly by hummocks, closed depressions, and the absence of an identifiable channel network. Following emplacement of the debris avalanche, a complex interrelationship of fluvial and mass wasting processes immediately began operating to return the impacted area to an equilibrium status through the removal of material (potential energy) and re-establishment of graded conditions. In an attempt to chronicle the morphologic evolution of this unique environmental setting, a systematic series of interpretative maps of several selected areas was produced. These maps, which document the rate and character of active geomorphic processes, are discussed.

Automated generation of massive image knowledge collections using Microsoft Live Labs Pivot to promote neuroimaging and translational research.

PubMed

Viangteeravat, Teeradache; Anyanwu, Matthew N; Ra Nagisetty, Venkateswara; Kuscu, Emin

2011-07-15

Massive datasets comprising high-resolution images, generated in neuro-imaging studies and in clinical imaging research, are increasingly challenging our ability to analyze, share, and filter such images in clinical and basic translational research. Pivot collection exploratory analysis provides each user the ability to fully interact with the massive amounts of visual data to fully facilitate sufficient sorting, flexibility and speed to fluidly access, explore or analyze the massive image data sets of high-resolution images and their associated meta information, such as neuro-imaging databases from the Allen Brain Atlas. It is used in clustering, filtering, data sharing and classifying of the visual data into various deep zoom levels and meta information categories to detect the underlying hidden pattern within the data set that has been used. We deployed prototype Pivot collections using the Linux CentOS running on the Apache web server. We also tested the prototype Pivot collections on other operating systems like Windows (the most common variants) and UNIX, etc. It is demonstrated that the approach yields very good results when compared with other approaches used by some researchers for generation, creation, and clustering of massive image collections such as the coronal and horizontal sections of the mouse brain from the Allen Brain Atlas. Pivot visual analytics was used to analyze a prototype of dataset Dab2 co-expressed genes from the Allen Brain Atlas. The metadata along with high-resolution images were automatically extracted using the Allen Brain Atlas API. It is then used to identify the hidden information based on the various categories and conditions applied by using options generated from automated collection. A metadata category like chromosome, as well as data for individual cases like sex, age, and plan attributes of a particular gene, is used to filter, sort and to determine if there exist other genes with a similar characteristics to Dab2. And online access to the mouse brain pivot collection can be viewed using the link http://edtech-dev.uthsc.edu/CTSI/teeDev1/unittest/PaPa/collection.html (user name: tviangte and password: demome) Our proposed algorithm has automated the creation of large image Pivot collections; this will enable investigators of clinical research projects to easily and quickly analyse the image collections through a perspective that is useful for making critical decisions about the image patterns discovered.

Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line

PubMed Central

STOCZYNSKA-FIDELUS, EWELINA; PIASKOWSKI, SYLWESTER; PAWLOWSKA, ROZA; SZYBKA, MALGORZATA; PECIAK, JOANNA; HULAS-BIGOSZEWSKA, KRYSTYNA; WINIECKA-KLIMEK, MARTA; RIESKE, PIOTR

2016-01-01

Thorough examination of genetic heterogeneity of cell lines is uncommon. In order to address this issue, the present study analyzed the genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia (T-ALL) cell line. For this purpose, traditional techniques such as fluorescence in situ hybridization and immunocytochemistry were used, in addition to more advanced techniques, including cell sorting, Sanger sequencing and massive parallel sequencing. The results indicated that the RPMI-8402 cell line consists of several genetically different cell subpopulations. Furthermore, massive parallel sequencing of RPMI-8402 provided insight into the evolution of T-ALL carcinogenesis, since this cell line exhibited the genetic heterogeneity typical of T-ALL. Therefore, the use of cell lines for drug testing in future studies may aid the progress of anticancer drug research. PMID:26870252

Q-sort assessment vs visual analog scale in the evaluation of smile esthetics.

PubMed

Schabel, Brian J; McNamara, James A; Franchi, Lorenzo; Baccetti, Tiziano

2009-04-01

This study was designed to compare the reliability of the Q-sort and visual analog scale (VAS) methods for the assessment of smile esthetics. Furthermore, agreement between orthodontists and parents of orthodontic patients, and between male and female raters, was assessed in terms of subjective evaluation of the smile. Clinical photographs and digital video captures of 48 orthodontically treated patients were rated by 2 panels: 25 experienced orthodontists (15 men, 10 women) and 20 parents of the patients (8 men, 12 women). Interrater reliability of the Q-sort and VAS methods was evaluated by using single-measure and average-measure intraclass correlation (ICC). Kappa agreement and the McNemar test were used to evaluate agreement between orthodontists and parents, and between men and women, for "attractive" and "unattractive" images of smiles captured with clinical photography. The single-measure ICC coefficients showed fair to good reliability of the Q-sort and poor reliability of the VAS for measuring esthetic preferences of an individual orthodontist or parent. Both rating groups agreed significantly (P >0.05) on the total percentage of "attractive" images of smiles captured with clinical photography. Men and women, however, significantly disagreed on the total percentages of "attractive" and "unattractive" smiles. Women rated higher percentages of both image groups as "attractive" than did their male counterparts. The Q-sort was more reliable than the VAS for measuring smile esthetics. Orthodontists and parents of orthodontic patients agreed with respect to "attractive" and "unattractive" smiles. Men and women agreed poorly with respect to "attractive" and "unattractive" smiles.

Studies of contemporary glacier basal ice cryostructures to identify buried basal ice in the permafrost: an example from the Matanuska Glacier, Alaska.

NASA Astrophysics Data System (ADS)

Stephani, E.; Fortier, D.; Kanevskiy, M.; Dillon, M.; Shur, Y.

2007-12-01

In the permafrost, massive ice bodies occur as buried glacier ice, aufeis ice, recrystalized snow, massive segregated ice, injection ice, ice wedges or ice formed in underground cavities ("pool ice", "thermokarst-cave ice"). The origin of massive ice bodies in the permafrost bears considerable implications for the reconstructions of paleoenvironments and paleoclimates. Our work aims to help the permafrost scientists working on massive icy sediments to distinguish buried basal glacier ice from other types of buried ice. To do so, the properties and structure of contemporary basal ice must be well known. Field investigations at the Matanuska Glacier (Chugach range, South-central Alaska), consisted in descriptions and sampling of natural basal ice exposures. We have used the basal ice facies classification of Lawson (1979) which is simple, easy to use in the field and provides a good framework for the description of basal ice exposures. Cores were extracted and brought back to the laboratory for water and grain-size analyses. The sediments forming the cryostructure were mostly polymodal, poorly sorted gravelly silt to gravelly fine sand, with mud contents generally over 50%. These data will be used to calibrate three-dimensional (3D) models produced from micro-tomographic scans of basal ice which will produce quantitative estimates of volumetric ice and sediments contents of basal ice cryostructures. Ultimately, visual qualitative and quantitative characterization of the basal ice components of 3D models together with field observations and laboratory analysis will allow for a new micro-facies and cryostructures classification of the basal ice. Our work will also have applications in glaciology, glacial geology, geomorphology, Quaternary and paleo-climatological studies based on inferences made from the structure of basal glacier ice. This paper presents the internal composition of the basal ice facies in terms of cryostructures assemblages (Fortier et al.: 2007) and sedimentological properties. Fortier, D., Kanevskiy, M, Stephani, E., Dillon, M., Shur, Y. 2007. Facies and cryostructures of glacier basal ice as an object of permafrost study, an example from the Matanuska Glacier, Alaska. Canadian Quaternary Association Conference, Ottawa, June 2007: 75. Lawson, D.E. 1979. Sedimentological analysis of the western terminus region of the Matanuska Glacier, Alaska. Cold Regions Engineering and Research Laboratory, Hanover, N.H., Report 79-9.

Apollo 16 soils - Grain size analyses and petrography

NASA Technical Reports Server (NTRS)

Heiken, G. H.; Mckay, D. S.; Fruland, R. M.

1973-01-01

Soils from South Ray Crater, North Ray Crater, and the interray area of Station 10 have a similar provenance, containing breccia fragments of low to medium metamorphic grade and low light/dark lithic fragment ratios; these appear to be characteristic of the Cayley Formation. The primary difference between soils possibly derived from North Ray and South Ray craters is in the agglutinate content. A soil from Stone Mountain (Station 4) is characterized by breccia fragments of medium to high metamorphic grade and a high light/dark lithic fragment ratio; this soil may be derived from the Descartes Formation. Differences between the selenomorphic units, the Descartes and Cayley formations, may be lithologic as well as structural. The mean grain size varies from 84 to 280 microns, and all of the samples are poorly to very poorly sorted. There appears to be a relation between the sorting, grain size, and agglutinate content, with the finer-grained, better sorted soils containing more than 30% agglutinates. 'Shadowed' soils, collected close to large boulders, are similar in all respects to the 'reference' soils collected at least 5 m from the boulders.

Eruption processes and deposit characteristics at the monogenetic Mt. Gambier Volcanic Complex, SE Australia: implications for alternating magmatic and phreatomagmatic activity

NASA Astrophysics Data System (ADS)

van Otterloo, Jozua; Cas, Raymond A. F.; Sheard, Malcolm J.

2013-08-01

The ˜5 ka Mt. Gambier Volcanic Complex in the Newer Volcanics Province, Australia is an extremely complex monogenetic, volcanic system that preserves at least 14 eruption points aligned along a fissure system. The complex stratigraphy can be subdivided into six main facies that record alternations between magmatic and phreatomagmatic eruption styles in a random manner. The facies are (1) coherent to vesicular fragmental alkali basalt (effusive/Hawaiian spatter and lava flows); (2) massive scoriaceous fine lapilli with coarse ash (Strombolian fallout); (3) bedded scoriaceous fine lapilli tuff (violent Strombolian fallout); (4) thin-medium bedded, undulating very fine lapilli in coarse ash (dry phreatomagmatic surge-modified fallout); (5) palagonite-altered, cross-bedded, medium lapilli to fine ash (wet phreatomagmatic base surges); and (6) massive, palagonite-altered, very poorly sorted tuff breccia and lapilli tuff (phreato-Vulcanian pyroclastic flows). Since most deposits are lithified, to quantify the grain size distributions (GSDs), image analysis was performed. The facies are distinct based on their GSDs and the fine ash to coarse+fine ash ratios. These provide insights into the fragmentation intensities and water-magma interaction efficiencies for each facies. The eruption chronology indicates a random spatial and temporal sequence of occurrence of eruption styles, except for a "magmatic horizon" of effusive activity occurring at both ends of the volcanic complex simultaneously. The eruption foci are located along NW-SE trending lineaments, indicating that the complex was fed by multiple dykes following the subsurface structures related to the Tartwaup Fault System. Possible factors causing vent migration along these dykes and changes in eruption styles include differences in magma ascent rates, viscosity, crystallinity, degassing and magma discharge rate, as well as hydrological parameters.

Evolution of a sediment wave in an experimental channel

Treesearch

Thomas E. Lisle; James E. Pizzuto; Hiroshi Ikeda; Fujiko Iseya; Yoshinori Kodama

1997-01-01

Abstract - The routing of bed material through channels is poorly understood. We approach the problem by observing and modeling the fate of a low-amplitude sediment wave of poorly sorted sand that we introduced into an experimental channel transporting sediment identical to that of the introduced wave. The wave essentially dispersed upstream and downstream without...

Dynamics of multiple bodies in a corotation resonance

NASA Astrophysics Data System (ADS)

A'Hearn, Joseph; Hedman, Matthew

2018-04-01

The orbital evolution of multiple massive bodies trapped in the same corotation resonance site has not yet been studied in depth, but could be relevant to the origins and history of small moons like Saturn's moon Aegaeon. We conduct numerical simulations of multiple bodies trapped within a corotation resonance and examine what happens to these bodies when they have close encounters. Compared to simulations with equal mass bodies, simulations with one body more massive than the others may be more likely to feature an asymmetry in the phase space of semi-major axis and mean longitude. That is, bodies on one side of phase space have a slightly greater tendency to lose angular momentum, while bodies on the other side gain angular momentum. With this asymmetry, the transfer of angular momentum during gravitational encounters makes it more likely for the most massive body rather than other bodies to approach the center of the corotation site. More work is needed to determine if this sort of process can significantly affect the orbital evolution of small moons like Aegaeon.

Lithofacies and seismic-reflection interpretation of temperate glacimarine sedimentation in Tarr Inlet, Glacier Bay, Alaska

USGS Publications Warehouse

Cai, J.; Powell, R.D.; Cowan, E.A.; Carlson, P.R.

1997-01-01

High-resolution seismic-reflection profiles of sediment fill within Tart Inlet of Glacier Bay, Alaska, show seismic facies changes with increasing distance from the glacial termini. Five types of seismic facies are recognized from analysis of Huntec and minisparker records, and seven lithofacies are determined from detailed sedimentologic study of gravity-, vibro- and box-cores, and bottom grab samples. Lithofacies and seismic facies associations, and fjord-floor morphology allow us to divide the fjord into three sedimentary environments: ice-proximal, iceberg-zone and ice-distal. The ice-proximal environment, characterized by a morainal-bank depositional system, can be subdivided into bank-back, bank-core and bank-front subenvironments, each of which is characterized by a different depositional subsystem. A bank-back subsystem shows chaotic seismic facies with a mounded surface, which we infer consists mainly of unsorted diamicton and poorly sorted coarse-grained sediments. A bank-core depositional subsystem is a mixture of diamicton, rubble, gravel, sand and mud. Seismic-reflection records of this subsystem are characterized by chaotic seismic facies with abundant hyperbolic diffractions and a hummocky surface. A bank-front depositional subsystem consists of mainly stratified and massive sand, and is characterized by internal hummocky facies on seismic-reflection records with significant surface relief and sediment gravity flow channels. The depositional system formed in the iceberg-zone environment consists of rhythmically laminated mud interbedded with thin beds of weakly stratified diamicton and stratified or massive sand and silt. On seismic-reflection profiles, this depositional system is characterized by discontinuously stratified facies with multiple channels on the surface in the proximal zone and a single channel on the largely flat sediment surface in the distal zone. The depositional system formed in the ice-distal environment consists of interbedded homogeneous or laminated mud and massive or stratified sand and coarse silt. This depositional system shows continuously stratified seismic facies with smooth and flat surfaces on minisparker records, and continuously stratified seismic facies which are interlayered with thin weakly stratified facies on Huntec records.

Optimized distributed systems achieve significant performance improvement on sorted merging of massive VCF files.

PubMed

Sun, Xiaobo; Gao, Jingjing; Jin, Peng; Eng, Celeste; Burchard, Esteban G; Beaty, Terri H; Ruczinski, Ingo; Mathias, Rasika A; Barnes, Kathleen; Wang, Fusheng; Qin, Zhaohui S

2018-06-01

Sorted merging of genomic data is a common data operation necessary in many sequencing-based studies. It involves sorting and merging genomic data from different subjects by their genomic locations. In particular, merging a large number of variant call format (VCF) files is frequently required in large-scale whole-genome sequencing or whole-exome sequencing projects. Traditional single-machine based methods become increasingly inefficient when processing large numbers of files due to the excessive computation time and Input/Output bottleneck. Distributed systems and more recent cloud-based systems offer an attractive solution. However, carefully designed and optimized workflow patterns and execution plans (schemas) are required to take full advantage of the increased computing power while overcoming bottlenecks to achieve high performance. In this study, we custom-design optimized schemas for three Apache big data platforms, Hadoop (MapReduce), HBase, and Spark, to perform sorted merging of a large number of VCF files. These schemas all adopt the divide-and-conquer strategy to split the merging job into sequential phases/stages consisting of subtasks that are conquered in an ordered, parallel, and bottleneck-free way. In two illustrating examples, we test the performance of our schemas on merging multiple VCF files into either a single TPED or a single VCF file, which are benchmarked with the traditional single/parallel multiway-merge methods, message passing interface (MPI)-based high-performance computing (HPC) implementation, and the popular VCFTools. Our experiments suggest all three schemas either deliver a significant improvement in efficiency or render much better strong and weak scalabilities over traditional methods. Our findings provide generalized scalable schemas for performing sorted merging on genetics and genomics data using these Apache distributed systems.

Challenges in automatic sorting of construction and demolition waste by hyperspectral imaging

NASA Astrophysics Data System (ADS)

Hollstein, Frank; Cacho, Íñigo; Arnaiz, Sixto; Wohllebe, Markus

2016-05-01

EU-28 countries currently generate 460 Mt/year of construction and demolition waste (C&DW) and the generation rate is expected to reach around 570 Mt/year between 2025 and 2030. There is great potential for recycling C&DW materials since they are massively produced and content valuable resources. But new C&DW is more complex than existing one and there is a need for shifting from traditional recycling approaches to novel recycling solutions. One basic step to achieve this objective is an improvement in (automatic) sorting technology. Hyperspectral Imaging is a promising candidate to support the process. However, the industrial distribution of Hyperspectral Imaging in the C&DW recycling branch is currently insufficiently pronounced due to high investment costs, still insufficient robustness of optical sensor hardware in harsh ambient conditions and, because of the need of sensor fusion, not well-engineered special software methods to perform the (on line) sorting tasks. Thereby frame rates of over 300 Hz are needed for a successful sorting result. Currently the biggest challenges with regard to C&DW detection cover the need of overlapping VIS, NIR and SWIR hyperspectral images in time and space, in particular for selective recognition of contaminated particles. In the study on hand a new approach for hyperspectral imagers is presented by exploiting SWIR hyperspectral information in real time (with 300 Hz). The contribution describes both laboratory results with regard to optical detection of the most important C&DW material composites as well as a development path for an industrial implementation in automatic sorting and separation lines. The main focus is placed on the closure of the two recycling circuits "grey to grey" and "red to red" because of their outstanding potential for sustainability in conservation of construction resources.

Optimized distributed systems achieve significant performance improvement on sorted merging of massive VCF files

PubMed Central

Gao, Jingjing; Jin, Peng; Eng, Celeste; Burchard, Esteban G; Beaty, Terri H; Ruczinski, Ingo; Mathias, Rasika A; Barnes, Kathleen; Wang, Fusheng

2018-01-01

Abstract Background Sorted merging of genomic data is a common data operation necessary in many sequencing-based studies. It involves sorting and merging genomic data from different subjects by their genomic locations. In particular, merging a large number of variant call format (VCF) files is frequently required in large-scale whole-genome sequencing or whole-exome sequencing projects. Traditional single-machine based methods become increasingly inefficient when processing large numbers of files due to the excessive computation time and Input/Output bottleneck. Distributed systems and more recent cloud-based systems offer an attractive solution. However, carefully designed and optimized workflow patterns and execution plans (schemas) are required to take full advantage of the increased computing power while overcoming bottlenecks to achieve high performance. Findings In this study, we custom-design optimized schemas for three Apache big data platforms, Hadoop (MapReduce), HBase, and Spark, to perform sorted merging of a large number of VCF files. These schemas all adopt the divide-and-conquer strategy to split the merging job into sequential phases/stages consisting of subtasks that are conquered in an ordered, parallel, and bottleneck-free way. In two illustrating examples, we test the performance of our schemas on merging multiple VCF files into either a single TPED or a single VCF file, which are benchmarked with the traditional single/parallel multiway-merge methods, message passing interface (MPI)–based high-performance computing (HPC) implementation, and the popular VCFTools. Conclusions Our experiments suggest all three schemas either deliver a significant improvement in efficiency or render much better strong and weak scalabilities over traditional methods. Our findings provide generalized scalable schemas for performing sorted merging on genetics and genomics data using these Apache distributed systems. PMID:29762754

Soil compaction vulnerability at Organ Pipe Cactus National Monument, Arizona

USGS Publications Warehouse

Webb, Robert H.; Nussear, Kenneth E.; Carmichael, Shinji; Esque, Todd C.

2014-01-01

Compaction vulnerability of different types of soils by hikers and vehicles is poorly known, particularly for soils of arid and semiarid regions. Engineering analyses have long shown that poorly sorted soils (for example, sandy loams) compact to high densities, whereas well-sorted soils (for example, eolian sand) do not compact, and high gravel content may reduce compaction. Organ Pipe Cactus National Monument (ORPI) in southwestern Arizona, is affected greatly by illicit activities associated with the United States–Mexico border, and has many soils that resource managers consider to be highly vulnerable to compaction. Using geospatial soils data for ORPI, compaction vulnerability was estimated qualitatively based on the amount of gravel and the degree of sorting of sand and finer particles. To test this qualitative assessment, soil samples were collected from 48 sites across all soil map units, and undisturbed bulk densities were measured. A scoring system was used to create a vulnerability index for soils on the basis of particle-size sorting, soil properties derived from Proctor compaction analyses, and the field undisturbed bulk densities. The results of the laboratory analyses indicated that the qualitative assessments of soil compaction vulnerability underestimated the area of high vulnerability soils by 73 percent. The results showed that compaction vulnerability of desert soils, such as those at ORPI, can be quantified using laboratory tests and evaluated using geographic information system analyses, providing a management tool that managers potentially could use to inform decisions about activities that reduce this type of soil disruption in protected areas.

Paleohydrology of Late Quaternary floods in the Atacama Desert and their paleoclimate implications

NASA Astrophysics Data System (ADS)

Izquierdo, Tatiana; Abad, Manuel; Larrondo, Lidisy

2017-04-01

The Quaternary fluvial succession in the Copiapó Valley (northern Chile) have not been deeply studied even though they record a large amount of palaeoenvironmental and paleoclimate information in an area of great interest as the Atacama Desert. The city of Copiapó is located at the confluence between Quebrada Paipote (the most important tributary of the middle course) and Copiapó River which has been dry during the last decades due to the surface and groundwater exploitation for agricultural and mining activity purposes upstream. Despite that, historical chronicles describe numerous flooding events in the city during the last 400 years due to snowmelt during the summer months or unusually intense rains during any time of the year. The most recent event occurred on March 25, 2015 when 70% of the city flooded and more than 2.2 million m3 of sediment accumulated, mostly coming from Quebrada Paipote. The sedimentological analysis of the lower fluvial terrace of the Copiapó River has allowed us to identify a fluvial system that abruptly changes upward to paleoflood and aeolian deposits. The latter constitute the top of the lower fluvial terraces on which the city of Copiapó is built. The fluvial facies are mainly formed by imbricated to massive conglomerates and poorly sorted pebble and cobble sized conglomerates with laminated sandstones that probably were deposited in a braided gravel-bed river. The overlying deposits are constituted by several levels of massive sandy siltstones and well sorted fine sands of aeolian origin that are interpreted as overbank flood events linked to flooding episodes that alternate with long episodes of eolian dunes and sand sheets development that buried almost the entire alluvial plain. This sharp change in the facies association record an abrupt climate change in the southern Atacama Desert during the recent Quaternary towards more arid conditions, with a dominance of floods and aeolian morphogenesis over the typical fluvial system processes in a semiarid environment. Several authors have proposed two episodes of regional changes in groundwater recharge tied to long-term changes in precipitation in the Atacama Desert when wet periods were terminated by pronounced dry periods, from 9 to 8 ka and from 3 to 0 ka. The change recorded in the fluvial succession of the Copiapó Valley can probably be linked to one of this climate event what would be confirmed by means of the deposits dating. In addition, this will provide more information for the estimation of the recurrence period for this catastrophic processes.

Automated generation of massive image knowledge collections using Microsoft Live Labs Pivot to promote neuroimaging and translational research

PubMed Central

2011-01-01

Background Massive datasets comprising high-resolution images, generated in neuro-imaging studies and in clinical imaging research, are increasingly challenging our ability to analyze, share, and filter such images in clinical and basic translational research. Pivot collection exploratory analysis provides each user the ability to fully interact with the massive amounts of visual data to fully facilitate sufficient sorting, flexibility and speed to fluidly access, explore or analyze the massive image data sets of high-resolution images and their associated meta information, such as neuro-imaging databases from the Allen Brain Atlas. It is used in clustering, filtering, data sharing and classifying of the visual data into various deep zoom levels and meta information categories to detect the underlying hidden pattern within the data set that has been used. Method We deployed prototype Pivot collections using the Linux CentOS running on the Apache web server. We also tested the prototype Pivot collections on other operating systems like Windows (the most common variants) and UNIX, etc. It is demonstrated that the approach yields very good results when compared with other approaches used by some researchers for generation, creation, and clustering of massive image collections such as the coronal and horizontal sections of the mouse brain from the Allen Brain Atlas. Results Pivot visual analytics was used to analyze a prototype of dataset Dab2 co-expressed genes from the Allen Brain Atlas. The metadata along with high-resolution images were automatically extracted using the Allen Brain Atlas API. It is then used to identify the hidden information based on the various categories and conditions applied by using options generated from automated collection. A metadata category like chromosome, as well as data for individual cases like sex, age, and plan attributes of a particular gene, is used to filter, sort and to determine if there exist other genes with a similar characteristics to Dab2. And online access to the mouse brain pivot collection can be viewed using the link http://edtech-dev.uthsc.edu/CTSI/teeDev1/unittest/PaPa/collection.html (user name: tviangte and password: demome) Conclusions Our proposed algorithm has automated the creation of large image Pivot collections; this will enable investigators of clinical research projects to easily and quickly analyse the image collections through a perspective that is useful for making critical decisions about the image patterns discovered. PMID:21884637

Lithofacies of Spencer Formation, western Tualatin Valley, Oregon

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van Atta, R.O.

The Spencer Formation crops out in a narrow band that trends north-northwest on the western edge of the Willamette and Tualatin Valleys, Oregon. It apparently conformably overlies mud rocks of either the Yamhill or the Nestucca Formation and is conformably overlain by the Pittsburgh Bluff Formation. The Spencer Formation consists of two members (informal): a lower highly micaceous sandstone (800-1000 ft) and an upper member that is micaceous siltstone and mudstone (1000-1300 ft). The lower member includes an upper part that is light-gray to creamy-gray, silty to muddy, pebbly lithic arkose to feldspathic litharenite, with minor arkose. Sorting is poormore » and beds may be laminated to ripple cross-laminated or massive and bioturbated with abundant mollusk shells, carbonized wood, and burrows. The lower part of the lower member is medium-gray to greenish-gray, silty, pumiceous lithic arkose to feldspathic litharenite. The texture tends to be more uniform and better sorted than that of the upper part of the member. Bedding is commonly massive due to bioturbation. The upper member is medium to dark-gray mudstone with thin pebble-conglomerate lenses. It intertongues with the lower member. Bioturbation, burrows, and carbonized wood are common. The trend in depositional environments appears to be from outer to mid-neritic (lower part, lower member) to shallow neritic, nearshore, and lagoonal (upper part, lower member, and upper member). The provenance of the Spencer Formation includes both proximal volcanics and distant plutonic and high-grade metamorphics.« less

Improving the Learning Design of Massive Open Online Courses

ERIC Educational Resources Information Center

Rubens, Wilfred

2014-01-01

Massive Open Online Courses (MOOCs) can be regarded as a promising next step in the evolution of distance education. However, they have been criticised for their poor learning design. This article describes the development of an adequate learning design in a series of nineteen MOOCs (called online master classes). A formative evaluation focuses on…

Sorted bedforms developed on sandy lobes fed by small ephemeral streams (Catalan continental shelf)

NASA Astrophysics Data System (ADS)

Durán, R.; Guillén, J.; Muñoz, A.; Guerrero, Q.

2016-12-01

The morphology and sedimentological characteristics of sorted bedforms identified in the Catalan continental shelf (NW Mediterranean Sea) have been characterized using multibeam echosounder data and sediment samples collected in 2013 within the FORMED project. Bathymetric data was compared with previous data gathered in 2004 within the ESPACE project to assess the decadal stability of these bedforms. The sorted bedforms were observed on the inner shelf at water depths from 10 to 40 m, along a coastal stretch of more than 3 km. They are associated with elongated patches of low backscatter, corresponding to fine sand. The fine-grained sediment patches are located off small bays fed by short, intermittent streams, extending down to 40 m water depth. The sorted bedforms exhibit elongated shapes with subtle relief (up to 1 m) and are oriented nearly perpendicular to the shoreline. In cross-section, the sorted bedforms display lateral symmetry in bathymetric relief and backscatter, with high backscatter corresponding to poorly sorted coarse sand (median size of 0.55-0.96 mm) centered on the bathymetric depression, and low backscatter consisting of well-sorted fine to medium sand (median sized of 0.22-0.35 mm) on the crest. The local input of well-sorted fine sand supplied by ephemeral streams over the coarse sand domain of the infralittoral prograding wedge contributes to the bed sediment heterogeneity (mixture of sediment), which is further reorganized into sorted bedforms. The sorted bedforms are better developed in deeper waters (20-40 m) than near the shoreline, probably due to stronger wave forcing in the shallower shelf that prevents the maintenance of these morphologies. At a decadal time scale, the morphological evolution of these bedforms indicates that they are persistent features, showing small changes in their boundaries, which is in agreement with previous observations and numerical simulations that highlighted the persistence and long-term stability of sorted bedforms at water depths greater than 15-20 m over annual or even decadal timescales.

Computations on the massively parallel processor at the Goddard Space Flight Center

NASA Technical Reports Server (NTRS)

Strong, James P.

1991-01-01

Described are four significant algorithms implemented on the massively parallel processor (MPP) at the Goddard Space Flight Center. Two are in the area of image analysis. Of the other two, one is a mathematical simulation experiment and the other deals with the efficient transfer of data between distantly separated processors in the MPP array. The first algorithm presented is the automatic determination of elevations from stereo pairs. The second algorithm solves mathematical logistic equations capable of producing both ordered and chaotic (or random) solutions. This work can potentially lead to the simulation of artificial life processes. The third algorithm is the automatic segmentation of images into reasonable regions based on some similarity criterion, while the fourth is an implementation of a bitonic sort of data which significantly overcomes the nearest neighbor interconnection constraints on the MPP for transferring data between distant processors.

Dissecting Cell-Type Composition and Activity-Dependent Transcriptional State in Mammalian Brains by Massively Parallel Single-Nucleus RNA-Seq.

PubMed

Hu, Peng; Fabyanic, Emily; Kwon, Deborah Y; Tang, Sheng; Zhou, Zhaolan; Wu, Hao

2017-12-07

Massively parallel single-cell RNA sequencing can precisely resolve cellular diversity in a high-throughput manner at low cost, but unbiased isolation of intact single cells from complex tissues such as adult mammalian brains is challenging. Here, we integrate sucrose-gradient-assisted purification of nuclei with droplet microfluidics to develop a highly scalable single-nucleus RNA-seq approach (sNucDrop-seq), which is free of enzymatic dissociation and nucleus sorting. By profiling ∼18,000 nuclei isolated from cortical tissues of adult mice, we demonstrate that sNucDrop-seq not only accurately reveals neuronal and non-neuronal subtype composition with high sensitivity but also enables in-depth analysis of transient transcriptional states driven by neuronal activity, at single-cell resolution, in vivo. Copyright © 2017 Elsevier Inc. All rights reserved.

Sorting Out the Ocean Crust Deep Biosphere with Single Cell Omics Approaches

NASA Astrophysics Data System (ADS)

Orcutt, B.; D'Angelo, T.; Goordial, J.; Jones, R. M.; Carr, S. A.

2017-12-01

Although oceanic crust comprises a large habitat for subsurface life, the structure, function, and dynamics of microbial communities living on rocks in the subsurface are poorly understood. Single cell level approaches can overcome limitations of low biomass in subsurface systems. Coupled with incubation experiments with amino acid orthologs, single cell level sorting can reveal high resolution information about identity, functional potential, and growth. Leveraging collaboration with the Single Cell Genomics Center and the Facility for Aquatic Cytometry at Bigelow Laboratory, we present recent results from single cell level sorting and -omics sequencing from several crustal environments, including the Atlantis Massif and the Juan de Fuca Ridge flank. We will also highlight new experiments conducted with samples recovered from the flank of the Mid-Atlantic Ridge.

Trending Fibrinolytic Dysregulation: Fibrinolysis Shutdown in the Days After Injury Is Associated With Poor Outcome in Severely Injured Children.

PubMed

Leeper, Christine M; Neal, Matthew D; McKenna, Christine J; Gaines, Barbara A

2017-09-01

To trend fibrinolysis after injury and determine the influence of traumatic brain injury (TBI) and massive transfusion on fibrinolysis status. Admission fibrinolytic derangement is common in injured children and adults, and is associated with poor outcome. No studies examine fibrinolysis days after injury. Prospective study of severely injured children at a level 1 pediatric trauma center. Rapid thromboelastography was obtained on admission and daily for up to 7 days. Standard definitions of hyperfibrinolysis (HF; LY30 ≥3), fibrinolysis shutdown (SD; LY30 ≤0.8), and normal (LY30 = 0.9-2.9) were applied. Antifibrinolytic use was documented. Outcomes were death, disability, and thromboembolic complications. Wilcoxon rank-sum and Fisher exact tests were performed. Exploratory subgroups included massively transfused and severe TBI patients. In all, 83 patients were analyzed with median (interquartile ranges) age 8 (4-12) and Injury Severity Score 22 (13-34), 73.5% blunt mechanism, 47% severe TBI, 20.5% massively transfused. Outcomes were 14.5% mortality, 43.7% disability, and 9.8% deep vein thrombosis. Remaining in or trending to SD was associated with death (P = 0.007), disability (P = 0.012), and deep vein thrombosis (P = 0.048). Median LY30 was lower on post-trauma day (PTD)1 to PTD4 in patients with poor compared with good outcome; median LY30 was lower on PTD1 to PTD3 in TBI patients compared with non-TBI patients. HF without associated shutdown was not related to poor outcome, but extreme HF (LY30 >30%, n = 3) was lethal. Also, 50% of massively transfused patients in hemorrhagic shock demonstrated SD physiology on admission. All with HF (fc31.2%) corrected after hemostatic resuscitation without tranexamic acid. Fibrinolysis shutdown is common postinjury and predicts poor outcomes. Severe TBI is associated with sustained shutdown. Empiric antifibrinolytics for children should be questioned; thromboelastography-directed selective use should be considered for documented HF.

Massive stars in the Sagittarius Dwarf Irregular Galaxy

NASA Astrophysics Data System (ADS)

Garcia, Miriam

2018-02-01

Low metallicity massive stars hold the key to interpret numerous processes in the past Universe including re-ionization, starburst galaxies, high-redshift supernovae, and γ-ray bursts. The Sagittarius Dwarf Irregular Galaxy [SagDIG, 12+log(O/H) = 7.37] represents an important landmark in the quest for analogues accessible with 10-m class telescopes. This Letter presents low-resolution spectroscopy executed with the Gran Telescopio Canarias that confirms that SagDIG hosts massive stars. The observations unveiled three OBA-type stars and one red supergiant candidate. Pending confirmation from high-resolution follow-up studies, these could be the most metal-poor massive stars of the Local Group.

Reverse shoulder arthroplasty for massive rotator cuff tear: risk factors for poor functional improvement.

PubMed

Hartzler, Robert U; Steen, Brandon M; Hussey, Michael M; Cusick, Michael C; Cottrell, Benjamin J; Clark, Rachel E; Frankle, Mark A

2015-11-01

Some patients unexpectedly have poor functional improvement after reverse shoulder arthroplasty (RSA) for massive rotator cuff tear without glenohumeral arthritis. Our aim was to identify risk factors for this outcome. We also assessed the value of RSA for cases with poor functional improvement vs. The study was a retrospective case-control analysis for primary RSA performed for massive rotator cuff tear without glenohumeral arthritis with minimum 2-year follow-up. Cases were defined as Simple Shoulder Test (SST) score improvement of ≤1, whereas controls improved SST score ≥2. Risk factors were chosen on the basis of previous association with poor outcomes after shoulder arthroplasty. Latissimus dorsi tendon transfer results were analyzed as a subgroup. Value was defined as improvement in American Shoulder and Elbow Surgeons (ASES) score per $10,000 hospital cost. In a multivariate binomial logistic regression analysis, neurologic dysfunction (P = .006), age <60 years (P = .02), and high preoperative SST score (P = .03) were independently associated with poor functional improvement. Latissimus dorsi tendon transfer patients significantly improved in active external rotation (-0.3° to 38.7°; P < .01). The value of RSA (ΔASES/$10,000 cost) for cases was 0.8 compared with 17.5 for controls (P < .0001). Young age, high preoperative function, and neurologic dysfunction were associated with poor functional improvement. Surgeons should consider these associations in counseling and selection of patients. Concurrent latissimus dorsi transfer was successful in restoring active external rotation in a subgroup of patients. The critical economic importance of improved patient selection is emphasized by the very low value of the procedure in the case group. Copyright © 2015 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

Glacial marine sediments in the precambrian Gowganda formation at Whitefish Falls, Ontario (Canada)

USGS Publications Warehouse

Lindsey, D.A.

1971-01-01

Study of a well-exposed section of the Gowganda Formation at Whitefish Falls, Ontario, suggests criteria for the recognition of glacial marine sediments. Thickness of hundreds of feet, lateral continuity, faint internal stratification, sorted lenses of sandstone and conglomerate, and dropstones characterize much of the tillite. Thickness of hundreds of feet, lateral continuity, and marked development of irregular and lenticular laminae instead of varve structure characterize much of the argillite. These characteristics, together with evidence for a nearshore, marine-to-deltaic environment for the overlying beds, suggest a glacial marine interpretation even though no fossil evidence is available. Massive tillite, tillite containing faint stratification and lenses of sorted conglomerate and sandstone, and dropstone-bearing argillite, all of which interfinger, suggest a glacial marine environment composed of: (1) a subglacial facies; (2) a periglacial facies; and (3) a facies of marine ice rafting, respectively. Separation of the two tillite-bearing members by as much as 700 ft. of argillite containing no dropstones suggests two distinct ice ages during Gowganda time. ?? 1971.

VizieR Online Data Catalog: Properties of OB associations in IC 1613 (Garcia+, 2010)

NASA Astrophysics Data System (ADS)

Garcia, M.; Herrero, A.; Castro, N.; Corral, L.; Rosenberg, A.

2014-06-01

To understand the structure and evolution of massive stars, systematic surveys of the Local Group galaxies have been undertaken, to find these objects in environments of different chemical abundances. We focus on the metal-poor irregular galaxy IC 1613 to analyze the stellar and wind structure of its low-metallicity massive stars. We ultimately aim to study the metallicity-dependent driving mechanism of the winds of blue massive stars and use metal-poor massive stars of the Local Volume as a proxy for the stars in the early Universe. In a previous paper we produced a list of OB associations in IC 1613. Their properties are not only a powerful aid towards finding the most interesting candidate massive stars, but also reveal the structure and recent star formation history of the galaxy. We characterize these OB associations and study their connection with the galactic global properties. The reddening-free Q parameter is a powerful tool in the photometric analysis of young populations of massive stars, since it exhibits a smaller degree of degeneracy with OB spectral types than the B-V color. The color-magnitude diagram (Q vs. V) of the OB associations in IC 1613 is studied to determine their age and mass, and confirm the population of young massive stars. We identified more than 10 stars with M>=50M⊙. Spectral classification available for some of them confirm their massive nature, yet we find the common discrepancy with the spectroscopically derived masses. There is a general increasing trend of the mass of the most massive member with the number of members of each association, but not with the stellar density. The average diameter of the associations of this catalog is 40pc, half the historically considered typical size of OB associations. Size increases with the association population. The distribution of the groups strongly correlates with that of neutral and ionized hydrogen. We find the largest dispersion of association ages in the bubble region of the galaxy where hydrogen is abundant, implying that recent star formation has proceeded over a longer period of time than in the rest of the galaxy, and is still ongoing. Very young associations are found at the west of the galaxy far from the bubble region, traditionally considered the sole locus of star formation, but still rich in neutral hydrogen. The contrast in the stellar properties derived from photometry and spectroscopy (when the latter is available) shows that the Q pseudo-color is very useful for estimating the parameters of OB stars when only photometric observations exist. This work helped define an extensive pool of candidate OB stars for subsequent spectroscopic analyses designed to study the structure and winds of metal-poor massive stars. (2 data files).

Characterization of bottom sediments in the Río de la Plata estuary

NASA Astrophysics Data System (ADS)

Simionato, Claudia G.; Moreira, Diego

2016-04-01

Bottom sediments and surface water samples were collected in the intermediate and outer Río de la Plata Estuary during 2009-2010, in six repeated cruises, with 26 stations each. Samples were processed for grain size using a laser particle size analyzer, and water and organic matter contents. The aim of this work is to analyze this data set to provide a comprehensive and objective characterization of the bottom sediments distribution, to study their composition and to progress in the construction of a conceptual model of the involved physical mechanisms. Principal Components Analysis is applied to the bottom sediments size histograms to investigate the spatial patterns. Variations in grain-size parameters contain information on possible sediment transport patterns, which were analyzed by means of trend vectors. Sediments show a gradational arrangement of textures, sand dominant at the head, silt in the intermediate estuary and clayey silt and clay at its mouth; textures become progressively more poorly sorted offshore, and the water and organic matter contents increase. And seem to be strongly related to the geometry and the hydrodynamics. Along the Northern coast of the intermediate estuary, well sorted medium and fine silt predominates, whereas in the Southern coast, coarser and less sorted silt prevails, due to differences in tidal currents and/or in water pathways. Around Barra del Indio, clay prevails over silt and sand, and the water and organic matter contents reach a maximum, probably due flocculation, and the reduction of the currents. Immediately seawards the salt wedge, net transport reverses its direction and well sorted coarser sand from the adjacent shelf dominates. Relict sediment is observed around the Santa Lucía River, consisting of poorly sorted fine silt and clay. The inferred net transport suggests convergence at the Barra del Indio shoal, which is consistent with the constant growing of the banks.

Placental Massive Perivillous Fibrinoid Deposition Associated with Coxsackievirus A16-Report of a Case, and Review of the Literature.

PubMed

Heller, Debra S; Tellier, Raymond; Pabbaraju, Kanti; Wong, Sallene; Faye-Petersen, Ona M; Muehlenbachs, Atis; Goldsmith, Cynthia; Denison, Amy; Zaki, Sherif R

Massive placental perivillous fibrinoid deposition in the placenta is thought to be an immune-related condition associated with poor perinatal outcomes, including growth restriction and intrauterine fetal demise, with a high risk of recurrence. Rare cases have been associated with Coxsackievirus infection. We present such a case and review the literature.

Poor Executive Functions among Children with Moderate-into-Severe Asthma: Evidence from WCST Performance.

PubMed

Taha, Haitham

2017-01-01

Executive functions (EFs) measures of 27 asthmatic children, with general learning difficulties, were tested by using the Wisconsin card sorting test (WCST), and were compared to the performances of 30 non-asthmatic children with general learning difficulties. The results revealed that the asthmatic group has poor performance through all the WCST psychometric parameters and especially the perseverative errors one. The results were discussed in light of the postulation that poor EFs could be associated with the learning difficulties of asthmatic children. Neurophysiological framework has been suggested to explain the etiology of poor EFs among children with moderate into severe asthma.

Hydraulic evolution of high-density turbidity currents from the Brushy Canyon Formation, Eddy County, New Mexico inferred by comparison to settling and sorting experiments

NASA Astrophysics Data System (ADS)

Motanated, Kannipa; Tice, Michael M.

2016-05-01

Hydraulic transformations in turbidity currents are commonly driven by or reflected in changes in suspended sediment concentrations, but changes preceding transformations can be difficult to diagnose because they do not produce qualitative changes in resultant deposits. This study integrates particle settling experiments and in situ detection of hydraulically contrasting particles in turbidites in order to infer changes in suspended sediment concentration during deposition of massive (Bouma Ta) sandstone divisions. Because grains of contrasting density are differentially sorted during hindered settling from dense suspensions, relative grading patterns can be used to estimate suspended sediment concentrations and interpret hydraulic evolution of the depositing turbidity currents. Differential settling of dense particles (aluminum ballotini) through suspensions of hydraulically coarser light particles (silica ballotini) with volumetric concentration, Cv, were studied in a thin vessel by using particle-image-velocimetry. At high Cv, aluminum particles were less retarded than co-sedimenting silica particles, and effectively settled as hydraulically coarser grains. This was because particles were entrained into clusters dominated by the settling behavior of the silica particles. Terminal settling velocities of both particles converged at Cv ≥ 25%, and particle sorting was diminished. The results of settling experiments were applied to understand settling of analogous feldspar and zircon grains in natural turbidity flows. Distributions of light and heavy mineral grains in massive sandstones, Bouma Ta divisions, of turbidites from the Middle Permian Brushy Canyon Formation were observed in situ by X-ray fluorescence microscopy (μXRF). Hydraulic sorting of these grains resulted in characteristic patterns of zirconium abundance that decreased from base to top within Ta divisions. These profiles resulted from upward fining of zircon grains with respect to co-occurring feldspar grains. Although calculated settling velocity distributions for zircon grains in structureless sandstones were slower than those for feldspar grains at infinite dilution, calculated settling velocity distributions for zircon and feldspar grains in overlying black siltstone layers were identical. This evidence suggests that these sandstone divisions were deposited from hyperconcentrated suspensions where particle segregation was diminished and hydraulically fine grains were entrained with hydraulically coarse particles. Hydraulic fining of zircon grains during deposition implies that the suspended sediment concentration at the bases of turbidity currents increased even as the overall current evolved toward lower density as reflected by cessation of Ta deposition and by hydraulic equivalence of zircon and feldspar grains in overlying low-density turbiditic siltstones. This evolution likely resulted from volumetric collapse of the turbidity currents.

How many neurons can we see with current spike sorting algorithms?

PubMed Central

Pedreira, Carlos; Martinez, Juan; Ison, Matias J.; Quian Quiroga, Rodrigo

2012-01-01

Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However, it is also possible that this is due to limitations of the spike sorting algorithms used to process the data. To address this issue, we used realistic simulations of extracellular recordings and found a relatively poor spike sorting performance for simulations containing a large number of neurons. In fact, the number of correctly identified neurons for single-channel recordings showed an asymptotic behavior saturating at about 8–10 units, when up to 20 units were present in the data. This performance was significantly poorer for neurons with low firing rates, as these units were twice more likely to be missed than the ones with high firing rates in simulations containing many neurons. These results uncover one of the main reasons for the relatively low number of neurons found in extracellular recording and also stress the importance of further developments of spike sorting algorithms. PMID:22841630

CC2D1A and CC2D1B regulate degradation and signaling of EGFR and TLR4.

PubMed

Deshar, Rakesh; Cho, Eun-Bee; Yoon, Sungjoo Kim; Yoon, Jong-Bok

2016-11-11

Signaling through many transmembrane receptors is terminated by their sorting to the intraluminal vesicles (ILVs) of multivescular bodies (MVBs) and subsequent lysosomal degradation. ILV formation requires the endosomal sorting complex required for transport (ESCRT) machinery. CC2D1A and CC2D1B interact with the CHMP4 family of proteins, the major subunit of the ESCRT-III complex, however, their roles in receptor degradation and signaling are poorly defined. Here, we report that CC2D1A binds to CHMP4B polymers formed on endosomes to regulate the endosomal sorting pathway. We show that depletion of CC2D1A and B accelerates degradation of EGFR and elicits rapid termination of its downstream signaling through ERK1 and 2. Depletion of CC2D1A and B promotes sorting of EGFR to ILV leading to its rapid lysosomal degradation. In addition, we show that knockdown of CC2D1A and B has similar effects on degradation and downstream signaling of another membrane receptor, TLR4. Thus, these findings suggest that CC2D1A and B may have broad effects on transmembrane receptors by preventing premature ILV sorting and termination of signaling. Copyright © 2016 Elsevier Inc. All rights reserved.

The Relationship Between Infrared Dark Cloud and Stellar Properties

NASA Astrophysics Data System (ADS)

Calahan, Jenny; Hora, Joseph L.

2018-01-01

Massive stars are known to form within infrared dark clouds (IRDCs), but many details about how molecular clouds collapse and form stars remain poorly understood.We determine the relationship between the dark cloud mass and the population of young stellar objects (YSOs) associated with the cloud to shed light on the physical processes occurring within these star forming regions. We chose to use a sample of IRDCs and YSOs within the Cygnus-X region, a close-by giant star formation complex that has every stage of star formation represented. Using observations from IRAC, MIPS, PACS, and SPIRE on Spitzer and Herschel we identified a sample of 30,903 YSOs and 167 IRDCs. We derived the class of each YSO as well as the mass of YSO and IRDCs from the flux information. Using these parameters, as well as their locations in the cloud, we were sorted IRDC fragments into larger filaments and associate a set of YSOs with each IRDC. By measuring and comparing parameters such as YSO total mass, number of YSOs, Class 0, Class I, and Class II populations, distance from host filament, and filament mass we tested for correlations between the YSO and IRDC parameters. Using this treasure trove of information, we find that Class 0 and I objects are located more closely to their host IRDC than their Class II counterparts. We also find that high-density IRDCs are better environments for star formation than low-density IRDCs. However, we find no correlation between the total mass of the IRDC and the largest YSO mass in the IRDC, suggesting that IRDCs of any mass can have massive YSOs associated with them.The SAO REU program is funded by the National Science Foundation REU and Department of Defense ASSURE programs under NSF Grant AST-1659473, and by the Smithsonian Institution.

Black hole demography at the dawn of gravitational-wave astronomy: state-of-the art and future perspectives

NASA Astrophysics Data System (ADS)

Mapelli, Michela

2018-02-01

The first four LIGO detections have confirmed the existence of massive black holes (BHs), with mass 30-40 M⊙. Such BHs might originate from massive metal-poor stars (Z < 0:3 Z⊙) or from gravitational instabilities in the early Universe. The formation channels of merging BHs are still poorly constrained. The measure of mass, spin and redshift distribution of merging BHs will give us fundamental clues to distinguish between different models. In parallel, a better understanding of several astrophysical processes (e.g. common envelope, core-collapse SNe, and dynamical evolution of BHs) is decisive, to shed light on the formation channels of merging BHs.

A sorting nexin 17-binding domain within the LRP1 cytoplasmic tail mediates receptor recycling through the basolateral sorting endosome

PubMed Central

Farfán, Pamela; Lee, Jiyeon; Larios, Jorge; Sotelo, Pablo; Bu, Guojun; Marzolo, María-Paz

2013-01-01

Sorting nexin 17 (SNX17) is an adaptor protein present in EEA1-positive sorting endosomes that promotes the efficient recycling of low-density lipoprotein receptor-related protein 1 (LRP1) to the plasma membrane through recognition of the first NPxY motif in the cytoplasmic tail of this receptor. The interaction of LRP1 with SNX17 also regulates the basolateral recycling of the receptor from the basolateral sorting endosome (BSE). In contrast, megalin, which is apically distributed in polarized epithelial cells and localizes poorly to EEA1-positive sorting endosomes, does not interact with SNX17, despite containing three NPxY motifs, indicating that this motif is not sufficient for receptor recognition by SNX17. Here, we identified a cluster of 32 amino acids within the cytoplasmic domain of LRP1 that is both necessary and sufficient for SNX17 binding. To delineate the function of this SNX17-binding domain, we generated chimeric proteins in which the SNX17-binding domain was inserted into the cytoplasmic tail of megalin. This insertion mediated the binding of megalin to SNX17 and modified the cell surface expression and recycling of megalin in non-polarized cells. However, the polarized localization of chimeric megalin was not modified in polarized MDCK cells. These results provide evidence regarding the molecular and cellular mechanisms underlying the specificity of SNX17-binding receptors and the restricted function of SNX17 in the BSE. PMID:23593972

Small-scale hero: Massive-star enrichment in the Hercules dwarf spheroidal

NASA Astrophysics Data System (ADS)

Koch, Andreas; Matteucci, Francesca; Feltzing, Sofia

2012-09-01

Dwarf spheroidal galaxies are often conjectured to be the sites of the first stars. The best current contenders for finding the chemical imprints from the enrichment by those massive objects are the ``ultrafaint dwarfs'' (UFDs). Here we present evidence for remarkably low heavy element abundances in the metal poor Hercules UFD. Combined with other peculiar abundance patterns this indicates that Hercules was likely only influenced by very few, massive explosive events - thus bearing the traces of an early, localized chemical enrichment with only very little other contributions from other sources at later times.

The mystery of a supposed massive star exploding in a brightest cluster galaxy

NASA Astrophysics Data System (ADS)

Hosseinzadeh, Griffin

2017-08-01

Most of the diversity of core-collapse supernovae results from late-stage mass loss by their progenitor stars. Supernovae that interact with circumstellar material (CSM) are a particularly good probe of these last stages of stellar evolution. Type Ibn supernovae are a rare and poorly understood class of hydrogen-poor explosions that show signs of interaction with helium-rich CSM. The leading hypothesis is that they are explosions of very massive Wolf-Rayet stars in which the supernova ejecta excites material previously lost by stellar winds. These massive stars have very short lifetimes, and therefore should only found in actively star-forming galaxies. However, PS1-12sk is a Type Ibn supernova found on the outskirts of a giant elliptical galaxy. As this is extraordinary unlikely, we propose to obtain deep UV images of the host environment of PS1-12sk in order to map nearby star formation and/or find a potential unseen star-forming host. If star formation is detected, its amount and location will provide deep insights into the progenitor picture for the poorly-understood Type Ibn class. If star formation is still not detected, these observations would challenge the well-accepted hypothesis that these are core-collapse supernovae at all.

The Rotator Cuff Organ: Integrating Developmental Biology, Tissue Engineering, and Surgical Considerations to Treat Chronic Massive Rotator Cuff Tears.

PubMed

Rothrauff, Benjamin B; Pauyo, Thierry; Debski, Richard E; Rodosky, Mark W; Tuan, Rocky S; Musahl, Volker

2017-08-01

The torn rotator cuff remains a persistent orthopedic challenge, with poor outcomes disproportionately associated with chronic, massive tears. Degenerative changes in the tissues that comprise the rotator cuff organ, including muscle, tendon, and bone, contribute to the poor healing capacity of chronic tears, resulting in poor function and an increased risk for repair failure. Tissue engineering strategies to augment rotator cuff repair have been developed in an effort to improve rotator cuff healing and have focused on three principal aims: (1) immediate mechanical augmentation of the surgical repair, (2) restoration of muscle quality and contractility, and (3) regeneration of native enthesis structure. Work in these areas will be reviewed in sequence, highlighting the relevant pathophysiology, developmental biology, and biomechanics, which must be considered when designing therapeutic applications. While the independent use of these strategies has shown promise, synergistic benefits may emerge from their combined application given the interdependence of the tissues that constitute the rotator cuff organ. Furthermore, controlled mobilization of augmented rotator cuff repairs during postoperative rehabilitation may provide mechanotransductive cues capable of guiding tissue regeneration and restoration of rotator cuff function. Present challenges and future possibilities will be identified, which if realized, may provide solutions to the vexing condition of chronic massive rotator cuff tears.

Fluvial sandstone reservoirs of Travis Peak (Hosston) Formation, east Texas basin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tye, R.S.

1989-03-01

Gas production (7.2 billion ft/sup 3/) from low-permeability sandstones in the Travis Peak Formation, North Appleby field, Nacogdoches County, Texas, is enhanced through massive hydraulic fracturing of stacked sandstones that occur at depths between 8000 and 10,000 ft. stratigraphic reservoirs were formed in multilateral tabular sandstones owing to impermeable mudstone interbeds that encase blocky to upward-fining sandstones. Pervasive quartz cement in the sandstones decreases porosity and permeability and augments the reservoir seal. Subsurface data indicate that much of this 2000-ft thick section represents aggradation of alluvial-valley deposits. Multiple channel belts form a network of overlapping, broad, tabular sandstones having thickness-to-widthmore » ratios of 1:850 (8-44 ft thick; widths exceed 4-5 mi). Six to eight channel belts, each containing 80-90% medium to fine-grained sandstone, can occupy a 200-ft thick interval. In a vertical sequence through one channel belt sandstone, basal planar cross-bedding grades upward into thinly interbedded sets of planar cross-beds and ripple cross-lamination. Clay-clast conglomerates line scoured channel bases. Adjacent to the channels, interbedded mudstones accumulated in well-drained swamps and lakes. Poorly sorted sandstones represent overbank deposition (crevasse splays and lacustrine deltas). During Travis Peak deposition, fluvial styles evolved from dominantly braided systems near the base of the formation to more mud-rich, meandering systems at the top.« less

Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol

PubMed Central

2017-01-01

Tight and tunable control of gene expression is a highly desirable goal in synthetic biology for constructing predictable gene circuits and achieving preferred phenotypes. Elucidating the sequence–function relationship of promoters is crucial for manipulating gene expression at the transcriptional level, particularly for inducible systems dependent on transcriptional regulators. Sort-seq methods employing fluorescence-activated cell sorting (FACS) and high-throughput sequencing allow for the quantitative analysis of sequence–function relationships in a robust and rapid way. Here we utilized a massively parallel sort-seq approach to analyze the formaldehyde-inducible Escherichia coli promoter (Pfrm) with single-nucleotide resolution. A library of mutated formaldehyde-inducible promoters was cloned upstream of gfp on a plasmid. The library was partitioned into bins via FACS on the basis of green fluorescent protein (GFP) expression level, and mutated promoters falling into each expression bin were identified with high-throughput sequencing. The resulting analysis identified two 19 base pair repressor binding sites, one upstream of the −35 RNA polymerase (RNAP) binding site and one overlapping with the −10 site, and assessed the relative importance of each position and base therein. Key mutations were identified for tuning expression levels and were used to engineer formaldehyde-inducible promoters with predictable activities. Engineered variants demonstrated up to 14-fold lower basal expression, 13-fold higher induced expression, and a 3.6-fold stronger response as indicated by relative dynamic range. Finally, an engineered formaldehyde-inducible promoter was employed to drive the expression of heterologous methanol assimilation genes and achieved increased biomass levels on methanol, a non-native substrate of E. coli. PMID:28463494

Field fertility of sex-sorted and non-sorted frozen-thawed stallion spermatozoa.

PubMed

Clulow, J R; Buss, H; Sieme, H; Rodger, J A; Cawdell-Smith, A J; Evans, G; Rath, D; Morris, L H A; Maxwell, W M C

2008-11-01

In the 2004/2005 breeding season, the fertility of sex-sorted (SS) and non-sorted (NS) frozen stallion spermatozoa from two Hannovarian stallions was compared. A hysteroscopic insemination technique [Morris, L.H., Tiplady, C., Allen, W.R., 2003a. Pregnancy rates in mares after a single fixed time hysteroscopic insemination of low numbers of frozen-thawed spermatozoa onto the uterotubal junction. Equine Vet. J. 35, 197-201] was used to deposit low doses (6, 13 or 25 x 10(6) frozen-thawed SS or NS spermatozoa) onto the utero-tubal junction at 32 or 38 h after the administration of Chorulon (2500 IU, Intervet). Fertility was low, with one pregnancy (13 x 10(6) spermatozoa, 500 microL) obtained after artificial insemination with frozen SS spermatozoa (n=29 cycles) which resulted in the birth of a filly. Two pregnancies were obtained in mares inseminated with 6 x 10(6) NS spermatozoa in 250 microL (n=31 cycles). Mares failing to conceive on two experimental cycles were allocated to the conventional insemination group. Insemination with >500 x 10(6) motile NS frozen-thawed spermatozoa, yielded satisfactory per cycle conception rates (35.5%, 22/62) for both stallions combined and was within the values of their normal fertility as quoted by the stud's records. This suggests that the quality of the frozen semen was acceptable and that the freezing processes yielded viable spermatozoa capable of fertilisation. The poor fertility after hysteroscopic insemination with low doses of sex-sorted or non-sorted spermatozoa from the same stallions may be directly attributable to the low dose insemination conditions with frozen-thawed rather than sex-sorted spermatozoa.

Noise-robust unsupervised spike sorting based on discriminative subspace learning with outlier handling.

PubMed

Keshtkaran, Mohammad Reza; Yang, Zhi

2017-06-01

Spike sorting is a fundamental preprocessing step for many neuroscience studies which rely on the analysis of spike trains. Most of the feature extraction and dimensionality reduction techniques that have been used for spike sorting give a projection subspace which is not necessarily the most discriminative one. Therefore, the clusters which appear inherently separable in some discriminative subspace may overlap if projected using conventional feature extraction approaches leading to a poor sorting accuracy especially when the noise level is high. In this paper, we propose a noise-robust and unsupervised spike sorting algorithm based on learning discriminative spike features for clustering. The proposed algorithm uses discriminative subspace learning to extract low dimensional and most discriminative features from the spike waveforms and perform clustering with automatic detection of the number of the clusters. The core part of the algorithm involves iterative subspace selection using linear discriminant analysis and clustering using Gaussian mixture model with outlier detection. A statistical test in the discriminative subspace is proposed to automatically detect the number of the clusters. Comparative results on publicly available simulated and real in vivo datasets demonstrate that our algorithm achieves substantially improved cluster distinction leading to higher sorting accuracy and more reliable detection of clusters which are highly overlapping and not detectable using conventional feature extraction techniques such as principal component analysis or wavelets. By providing more accurate information about the activity of more number of individual neurons with high robustness to neural noise and outliers, the proposed unsupervised spike sorting algorithm facilitates more detailed and accurate analysis of single- and multi-unit activities in neuroscience and brain machine interface studies.

Noise-robust unsupervised spike sorting based on discriminative subspace learning with outlier handling

NASA Astrophysics Data System (ADS)

Keshtkaran, Mohammad Reza; Yang, Zhi

2017-06-01

Objective. Spike sorting is a fundamental preprocessing step for many neuroscience studies which rely on the analysis of spike trains. Most of the feature extraction and dimensionality reduction techniques that have been used for spike sorting give a projection subspace which is not necessarily the most discriminative one. Therefore, the clusters which appear inherently separable in some discriminative subspace may overlap if projected using conventional feature extraction approaches leading to a poor sorting accuracy especially when the noise level is high. In this paper, we propose a noise-robust and unsupervised spike sorting algorithm based on learning discriminative spike features for clustering. Approach. The proposed algorithm uses discriminative subspace learning to extract low dimensional and most discriminative features from the spike waveforms and perform clustering with automatic detection of the number of the clusters. The core part of the algorithm involves iterative subspace selection using linear discriminant analysis and clustering using Gaussian mixture model with outlier detection. A statistical test in the discriminative subspace is proposed to automatically detect the number of the clusters. Main results. Comparative results on publicly available simulated and real in vivo datasets demonstrate that our algorithm achieves substantially improved cluster distinction leading to higher sorting accuracy and more reliable detection of clusters which are highly overlapping and not detectable using conventional feature extraction techniques such as principal component analysis or wavelets. Significance. By providing more accurate information about the activity of more number of individual neurons with high robustness to neural noise and outliers, the proposed unsupervised spike sorting algorithm facilitates more detailed and accurate analysis of single- and multi-unit activities in neuroscience and brain machine interface studies.

The Road not Taken: Less Traveled Roads from the TGN to the Plasma Membrane

PubMed Central

Spang, Anne

2015-01-01

The trans-Golgi network functions in the distribution of cargo into different transport vesicles that are destined to endosomes, lysosomes and the plasma membrane. Over the years, it has become clear that more than one transport pathway promotes plasma membrane localization of proteins. In spite of the importance of temporal and spatial control of protein localization at the plasma membrane, the regulation of sorting into and the formation of different transport containers are still poorly understood. In this review different transport pathways, with a special emphasis on exomer-dependent transport, and concepts of regulation and sorting at the TGN are discussed. PMID:25764365

The Road not Taken: Less Traveled Roads from the TGN to the Plasma Membrane.

PubMed

Spang, Anne

2015-03-10

The trans-Golgi network functions in the distribution of cargo into different transport vesicles that are destined to endosomes, lysosomes and the plasma membrane. Over the years, it has become clear that more than one transport pathway promotes plasma membrane localization of proteins. In spite of the importance of temporal and spatial control of protein localization at the plasma membrane, the regulation of sorting into and the formation of different transport containers are still poorly understood. In this review different transport pathways, with a special emphasis on exomer-dependent transport, and concepts of regulation and sorting at the TGN are discussed.

Microparticles controllable accumulation, arrangement, and spatial shaping performed by tapered-fiber-based laser-induced convection flow.

PubMed

Zhang, Yu; Lei, Jiaojie; Zhang, Yaxun; Liu, Zhihai; Zhang, Jianzhong; Yang, Xinghua; Yang, Jun; Yuan, Libo

2017-10-30

The ability to arrange cells and/or microparticles into the desired pattern is critical in biological, chemical, and metamaterial studies and other applications. Researchers have developed a variety of patterning techniques, which either have a limited capacity to simultaneously trap massive particles or lack the spatial resolution necessary to manipulate individual particle. Several approaches have been proposed that combine both high spatial selectivity and high throughput simultaneously. However, those methods are complex and difficult to fabricate. In this article, we propose and demonstrate a simple method that combines the laser-induced convection flow and fiber-based optical trapping methods to perform both regular and special spatial shaping arrangement. Essentially, we combine a light field with a large optical intensity gradient distribution and a thermal field with a large temperature gradient distribution to perform the microparticles shaping arrangement. The tapered-fiber-based laser-induced convection flow provides not only the batch manipulation of massive particles, but also the finer manipulation of special one or several particles, which break out the limit of single-fiber-based massive/individual particles photothermal manipulation. The combination technique allows for microparticles quick accumulation, single-layer and multilayer arrangement; special spatial shaping arrangement/adjustment, and microparticles sorting.

Paroxysmal dome explosion during the Merapi 2010 eruption: Processes and facies relationships of associated high-energy pyroclastic density currents

NASA Astrophysics Data System (ADS)

Komorowski, Jean-Christophe; Jenkins, Susanna; Baxter, Peter J.; Picquout, Adrien; Lavigne, Franck; Charbonnier, Sylvain; Gertisser, Ralf; Preece, Katie; Cholik, Noer; Budi-Santoso, Agus; Surono

2013-07-01

An 11-minute sequence of laterally-directed explosions and retrogressive collapses on 5 November 2010 at Merapi (Indonesia) destroyed a rapidly-growing dome and generated high-energy pyroclastic density currents (PDCs) spreading over 22 km2 with a runout of 8.4 km while contemporaneous co-genetic valley-confined PDCs reached 15.5 km. This event formed Stage 4 of the multi-stage 2010 eruption, the most intense eruptive episode at Merapi since 1872. The deposits and the widespread devastating impact of associated high-energy PDCs on trees and buildings show striking similarities with those from historical volcanic blasts (Montagne Pelée, Martinique, Bezymianny, Russia, Mount St. Helens, USA, Soufrière Hills, Montserrat). We provide data from stratigraphic and sedimentologic analyses of 62 sections of the first unequivocal blast-like deposits in Merapi's recent history. We used high resolution satellite imagery to map eruptive units and flow direction from the pattern of extensive tree blowdown. The stratigraphy of Stage 4 consists of three depositional units (U0, U1, U2) that we correlate to the second, third and fourth explosions of the seismic record. Both U1 and U2 show a bi-partite layer stratigraphy consisting each of a lower L1 layer and an upper L2 layer. The lower L1 layer is typically very coarse-grained, fines-poor, poorly-sorted and massive, and was deposited by the erosive waxing flow head. The overlying L2 layer is much finer grained, fines-rich, moderately to well-sorted, with laminar to wavy stratification. L2 was deposited from the waning upper part and wake of the PDC. Field observations indicate that PDC height reached ~ 330 m with an internal velocity of ~ 100 m s- 1 within 3 km from the source. The summit's geometry and the terrain morphology formed by a major transversal ridge and a funneling deep canyon strongly focused PDC mass towards a major constriction, thereby limiting the loss of kinetic energy. This favored elevated PDC velocities and high particle concentration, promoted overspilling of PDCs across high ridges into other river valleys, and generated significant dynamic pressures to distances of 6 km that caused total destruction of buildings and the forest. The Merapi 2010 eruption highlights that explosive and gravitational disintegration of a rapidly growing dome can generate devastating high-energy, high-velocity PDCs. This constitutes a credible high impact scenario for future multi-stage eruptions at Merapi and at other volcanoes that pose particular monitoring, crisis response, and risk reduction challenges.

Arthroscopic Repair for Chronic Massive Rotator Cuff Tears: A Systematic Review.

PubMed

Henry, Patrick; Wasserstein, David; Park, Sam; Dwyer, Tim; Chahal, Jaskarndip; Slobogean, Gerard; Schemitsch, Emil

2015-12-01

To systematically review the available evidence for arthroscopic repair of chronic massive rotator cuff tears and identify patient demographics, pre- and post-operative functional limitations, reparability and repair techniques, and retear rates. Medline, Embase, the Cochrane Database of Systematic Reviews, and the Cochrane Central Register of Controlled Trials were searched to identify all clinical papers describing arthroscopic repair of chronic massive rotator cuff tears. Papers were excluded if a definition of "massive" was not provided, if the definition of "massive" was considered inappropriate by agreement between the 2 reviewers, or if patients with smaller tears were also included in the study population. Study quality and clinical outcome data were pooled and summarized. There were 18 papers that met the eligibility criteria; they involved 954 patients with a mean age of 63 (range, 37 to 87), 48% of whom were female. There were 5 prospective and 13 retrospective study designs. The overall study quality was poor according to the Modified Coleman Methodology Score. Of the 954 repairs, 81% were complete repairs and 19% were partial repairs. The follow-up range was between 33 and 52 months, and the mean duration between symptom onset and surgery was 24 months. Single-row repairs were performed in 56% or patients, and double-row repairs were performed in 44%. A pooled analysis demonstrated an improvement in visual analog scale from 5.9 to 1.7, active range of motion from 125° to 169°, and the Constant-Murley score from 49 to 74. The pooled retear rate was 79%. Arthroscopic repair of chronic massive rotator cuff tears is associated with complete repair in the majority of cases and consistently improves pain, range of motion, and functional outcome scores; however, the retear rate is high. Existing research on massive rotator cuff repair is limited to poor- to fair-quality studies. Level IV, systematic review including Level IV studies. Copyright © 2015 Arthroscopy Association of North America. Published by Elsevier Inc. All rights reserved.

Open-Source Sequence Clustering Methods Improve the State Of the Art.

PubMed

Kopylova, Evguenia; Navas-Molina, Jose A; Mercier, Céline; Xu, Zhenjiang Zech; Mahé, Frédéric; He, Yan; Zhou, Hong-Wei; Rognes, Torbjørn; Caporaso, J Gregory; Knight, Rob

2016-01-01

Sequence clustering is a common early step in amplicon-based microbial community analysis, when raw sequencing reads are clustered into operational taxonomic units (OTUs) to reduce the run time of subsequent analysis steps. Here, we evaluated the performance of recently released state-of-the-art open-source clustering software products, namely, OTUCLUST, Swarm, SUMACLUST, and SortMeRNA, against current principal options (UCLUST and USEARCH) in QIIME, hierarchical clustering methods in mothur, and USEARCH's most recent clustering algorithm, UPARSE. All the latest open-source tools showed promising results, reporting up to 60% fewer spurious OTUs than UCLUST, indicating that the underlying clustering algorithm can vastly reduce the number of these derived OTUs. Furthermore, we observed that stringent quality filtering, such as is done in UPARSE, can cause a significant underestimation of species abundance and diversity, leading to incorrect biological results. Swarm, SUMACLUST, and SortMeRNA have been included in the QIIME 1.9.0 release. IMPORTANCE Massive collections of next-generation sequencing data call for fast, accurate, and easily accessible bioinformatics algorithms to perform sequence clustering. A comprehensive benchmark is presented, including open-source tools and the popular USEARCH suite. Simulated, mock, and environmental communities were used to analyze sensitivity, selectivity, species diversity (alpha and beta), and taxonomic composition. The results demonstrate that recent clustering algorithms can significantly improve accuracy and preserve estimated diversity without the application of aggressive filtering. Moreover, these tools are all open source, apply multiple levels of multithreading, and scale to the demands of modern next-generation sequencing data, which is essential for the analysis of massive multidisciplinary studies such as the Earth Microbiome Project (EMP) (J. A. Gilbert, J. K. Jansson, and R. Knight, BMC Biol 12:69, 2014, http://dx.doi.org/10.1186/s12915-014-0069-1).

Rab15 Effector Protein: A Novel Protein for Receptor Recycling from the Endocytic Recycling CompartmentD⃞

PubMed Central

Strick, David J.; Elferink, Lisa A.

2005-01-01

Sorting endosomes and the endocytic recycling compartment are critical intracellular stores for the rapid recycling of internalized membrane receptors to the cell surface in multiple cell types. However, the molecular mechanisms distinguishing fast receptor recycling from sorting endosomes and slow receptor recycling from the endocytic recycling compartment remain poorly understood. We previously reported that Rab15 differentially regulates transferrin receptor trafficking through sorting endosomes and the endocytic recycling compartment, suggesting a role for distinct Rab15-effector interactions at these endocytic compartments. In this study, we identified the novel protein Rab15 effector protein (REP15) as a binding partner for Rab15-GTP. REP15 is compartment specific, colocalizing with Rab15 and Rab11 on the endocytic recycling compartment but not with Rab15, Rab4, or early endosome antigen 1 on sorting endosomes. REP15 interacts directly with Rab15-GTP but not with Rab5 or Rab11. Consistent with its localization, REP15 overexpression and small interfering RNA-mediated depletion inhibited transferrin receptor recycling from the endocytic recycling compartment, without affecting receptor entry into or recycling from sorting endosomes. Our data identify REP15 as a compartment-specific protein for receptor recycling from the endocytic recycling compartment, highlighting that the rapid and slow modes of transferrin receptor recycling are mechanistically distinct pathways. PMID:16195351

Low-metallicity (sub-SMC) massive stars

NASA Astrophysics Data System (ADS)

Garcia, Miriam; Herrero, Artemio; Najarro, Francisco; Camacho, Inés; Lennon, Daniel J.; Urbaneja, Miguel A.; Castro, Norberto

2017-11-01

The double distance and metallicity frontier marked by the SMC has been finally broken with the aid of powerful multi-object spectrographs installed at 8-10m class telescopes. VLT, GTC and Keck have enabled studies of massive stars in dwarf irregular galaxies of the Local Group with poorer metal-content than the SMC. The community is working to test the predictions of evolutionary models in the low-metallicity regime, set the new standard for the metal-poor high-redshift Universe, and test the extrapolation of the physics of massive stars to environments of decreasing metallicity. In this paper, we review current knowledge on this topic.

Modern sedimentary facies, depositional environments, and major controlling processes on an arid siliciclastic coast, Al qahmah, SE Red Sea, Saudi Arabia

NASA Astrophysics Data System (ADS)

Nabhan, Abdullah I.; Yang, Wan

2018-04-01

The facies and environments along the arid siliciclastic coast of Red Sea in Al Qahmah, Saudi Arabia are studied to establish a depositional model for interpretation of ancient rocks deposited in rift settings. Field and petrographic studies of 151 sediment samples in an area of 20 km2 define seven main facies types: beach, washover fan, tidal channel, dune, sabkha, delta, and wadi (seasonal stream). The wadi and delta facies are composed of poorly to moderately well-sorted, gravelly, medium-to-fine sands. Delta-front sands are redistributed by southward longshore currents to form a beach. Beach facies is composed of well-to-moderately sorted fine sands with minor gravels, which contain high concentrations of magnetite, ilmenite, garnet, pyroxene, amphibole, epidote, titanite, and apatite grains, indicating strong winnowing. Crabs and other burrowers destroy primary sedimentary structures and mix sediments in foreshore and backshore of the beaches. Wind and storm surge rework foreshore and backshore sediments to form washover fans. Sabkha facies occurs extensively in supratidal depressions behind beach, are flooded by rainstorms and spring tide, and capped by a 5-cm-thick crust composed of interlaminated halite, quartz, albite, minor gypsum and biotite, and rarely calcium carbonate. Halite occurs as thin sheets and gypsum as nodules with a chicken-wire structure. Clastic fraction in sabkha sediments ranges from coarse silt to coarse sand with moderate sorting, and is transported by currents and wind. Tidal inlets and tidal creeks assume abandoned wadis and are filled by muddy sand. Sand dunes and sand sheets are 1-7 m high and widely distributed due to variable wind directions. Fine-grained dune sands are moderately well sorted, whereas sheet sands are coarser and poorly sorted due to vegetation baffling. Most eolian sands are sourced from beach deposits. This suite of complex riverine, wave, tidal, wind, chemical, and biological processes form the facies mosaic along the arid Al Qahmah coast, which is strongly affected by climate-driven evaporation and wind action.

How many neurons can we see with current spike sorting algorithms?

PubMed

Pedreira, Carlos; Martinez, Juan; Ison, Matias J; Quian Quiroga, Rodrigo

2012-10-15

Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However, it is also possible that this is due to limitations of the spike sorting algorithms used to process the data. To address this issue, we used realistic simulations of extracellular recordings and found a relatively poor spike sorting performance for simulations containing a large number of neurons. In fact, the number of correctly identified neurons for single-channel recordings showed an asymptotic behavior saturating at about 8-10 units, when up to 20 units were present in the data. This performance was significantly poorer for neurons with low firing rates, as these units were twice more likely to be missed than the ones with high firing rates in simulations containing many neurons. These results uncover one of the main reasons for the relatively low number of neurons found in extracellular recording and also stress the importance of further developments of spike sorting algorithms. Copyright © 2012 Elsevier B.V. All rights reserved.

A sorting nexin 17-binding domain within the LRP1 cytoplasmic tail mediates receptor recycling through the basolateral sorting endosome.

PubMed

Farfán, Pamela; Lee, Jiyeon; Larios, Jorge; Sotelo, Pablo; Bu, Guojun; Marzolo, María-Paz

2013-07-01

Sorting nexin 17 (SNX17) is an adaptor protein present in early endosomal antigen 1 (EEA1)-positive sorting endosomes that promotes the efficient recycling of low-density lipoprotein receptor-related protein 1 (LRP1) to the plasma membrane through recognition of the first NPxY motif in the cytoplasmic tail of this receptor. The interaction of LRP1 with SNX17 also regulates the basolateral recycling of the receptor from the basolateral sorting endosome (BSE). In contrast, megalin, which is apically distributed in polarized epithelial cells and localizes poorly to EEA1-positive sorting endosomes, does not interact with SNX17, despite containing three NPxY motifs, indicating that this motif is not sufficient for receptor recognition by SNX17. Here, we identified a cluster of 32 amino acids within the cytoplasmic domain of LRP1 that is both necessary and sufficient for SNX17 binding. To delineate the function of this SNX17-binding domain, we generated chimeric proteins in which the SNX17-binding domain was inserted into the cytoplasmic tail of megalin. This insertion mediated the binding of megalin to SNX17 and modified the cell surface expression and recycling of megalin in non-polarized cells. However, the polarized localization of chimeric megalin was not modified in polarized Madin-Darby canine kidney cells. These results provide evidence regarding the molecular and cellular mechanisms underlying the specificity of SNX17-binding receptors and the restricted function of SNX17 in the BSE. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Ultraviolet fluorescence to identify navel oranges with poor peel quality and decay

USDA-ARS?s Scientific Manuscript database

Navel oranges were sorted into four groups under ultraviolet (UV) illumination in commercial packinghouse black light rooms based upon the amount of fluorescence visible on each fruit to determine if fluorescence was predictive of peel quality. The groups corresponded to fruit with: 1) no fluorescen...

Helping Women into Work

ERIC Educational Resources Information Center

Ward, Jane

2011-01-01

With women bearing a disproportionate share of economic hardship, their poor representation on training designed to tackle barriers to work is a critical concern. The author asks what can be done to improve women's access to this sort of learning. As the underrecruitment of women to ESF pre-employment programmes demonstrates, the author suggests…

Supporting the Students of the Future

ERIC Educational Resources Information Center

Navarro, Diego

2012-01-01

Underprepared students come to community colleges and many four-year colleges because they have hope for a better life and the courage to attempt something difficult. But having dreams and aspirations is not enough: Without some sort of intervention and support, a student's chances of completing college are poor. Many challenged students…

Welfare, Work and Raising Children: Conversations with Twenty-One Maine Families.

ERIC Educational Resources Information Center

Hastedt, Christine B.; Smith, Rebekah J.

Five years after the massive overhaul of the nation's welfare system, 21 Maine families receiving public assistance spoke about their lives during welfare reform. The following were among the key themes that emerged throughout the conversations: (1) those leaving Temporary Assistance for Needy Families (TANF) often remain poor or very nearly poor;…

How does creating a concept map affect item-specific encoding?

PubMed

Grimaldi, Phillip J; Poston, Laurel; Karpicke, Jeffrey D

2015-07-01

Concept mapping has become a popular learning tool. However, the processes underlying the task are poorly understood. In the present study, we examined the effect of creating a concept map on the processing of item-specific information. In 2 experiments, subjects learned categorized or ad hoc word lists by making pleasantness ratings, sorting words into categories, or creating a concept map. Memory was tested using a free recall test and a recognition memory test, which is considered to be especially sensitive to item-specific processing. Typically, tasks that promote item-specific processing enhance free recall of categorized lists, relative to category sorting. Concept mapping resulted in lower recall performance than both the pleasantness rating and category sorting condition for categorized words. Moreover, concept mapping resulted in lower recognition memory performance than the other 2 tasks. These results converge on the conclusion that creating a concept map disrupts the processing of item-specific information. (c) 2015 APA, all rights reserved.

Grain size analysis and depositional environment of shallow marine to basin floor, Kelantan River Delta

NASA Astrophysics Data System (ADS)

Afifah, M. R. Nurul; Aziz, A. Che; Roslan, M. Kamal

2015-09-01

Sediment samples were collected from the shallow marine from Kuala Besar, Kelantan outwards to the basin floor of South China Sea which consisted of quaternary bottom sediments. Sixty five samples were analysed for their grain size distribution and statistical relationships. Basic statistical analysis like mean, standard deviation, skewness and kurtosis were calculated and used to differentiate the depositional environment of the sediments and to derive the uniformity of depositional environment either from the beach or river environment. The sediments of all areas were varied in their sorting ranging from very well sorted to poorly sorted, strongly negative skewed to strongly positive skewed, and extremely leptokurtic to very platykurtic in nature. Bivariate plots between the grain-size parameters were then interpreted and the Coarsest-Median (CM) pattern showed the trend suggesting relationships between sediments influenced by three ongoing hydrodynamic factors namely turbidity current, littoral drift and waves dynamic, which functioned to control the sediments distribution pattern in various ways.

Neonatal Intensive-Care Unit Graduates Show Persistent Difficulties in an Intra-Dimensional Shift Card Sort

PubMed Central

Kittler, Phyllis M.; Brooks, Patricia J.; Rossi, Vanessa; Karmel, Bernard Z.; Gardner, Judith M.; Flory, Michael J.

2014-01-01

Neonatal intensive-care unit (NICU) graduates, a group at risk for attention problems and ADHD, performed an intra-dimensional shift card sort at 34, 42, 51, and 60 months to assess executive function and to examine effects of individual risk factors. In the ‘silly’ game, children sorted cards (airplanes and dogs) so they were not the same as targets. In the ‘same’ game they did the opposite. Performance on the ‘silly’ game was poor, especially when it was presented first. Success in following ‘silly’ game rules improved with age, and was significantly linked to maternal education and birth weight for gestational age, a measure of intrauterine stress. Degree of CNS injury differentiated children who completed the task from children who did not, and also affected the need to repeat instructions in the ‘same’ game. These results confirm an increased likelihood of impairments in executive function during preschool years in NICU graduates. PMID:24683313

Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum.

PubMed

Uesaka, Naofumi; Abe, Manabu; Konno, Kohtarou; Yamazaki, Maya; Sakoori, Kazuto; Watanabe, Takaki; Kao, Tzu-Huei; Mikuni, Takayasu; Watanabe, Masahiko; Sakimura, Kenji; Kano, Masanobu

2018-02-21

Elimination of redundant synapses formed early in development and strengthening of necessary connections are crucial for shaping functional neural circuits. Purkinje cells (PCs) in the neonatal cerebellum are innervated by multiple climbing fibers (CFs) with similar strengths. A single CF is strengthened whereas the other CFs are eliminated in each PC during postnatal development. The underlying mechanisms, particularly for the strengthening of single CFs, are poorly understood. Here we report that progranulin, a multi-functional growth factor implicated in the pathogenesis of frontotemporal dementia, strengthens developing CF synaptic inputs and counteracts their elimination from postnatal day 11 to 16. Progranulin derived from PCs acts retrogradely onto its putative receptor Sort1 on CFs. This effect is independent of semaphorin 3A, another retrograde signaling molecule that counteracts CF synapse elimination. We propose that progranulin-Sort1 signaling strengthens and maintains developing CF inputs, and may contribute to selection of single "winner" CFs that survive synapse elimination. Copyright © 2018 Elsevier Inc. All rights reserved.

GGA3 mediates TrkA endocytic recycling to promote sustained Akt phosphorylation and cell survival

PubMed Central

Li, Xuezhi; Lavigne, Pierre; Lavoie, Christine

2015-01-01

Although TrkA postendocytic sorting significantly influences neuronal cell survival and differentiation, the molecular mechanism underlying TrkA receptor sorting in the recycling or degradation pathways remains poorly understood. Here we demonstrate that Golgi-localized, γ adaptin-ear–containing ADP ribosylation factor-binding protein 3 (GGA3) interacts directly with the TrkA cytoplasmic tail through an internal DXXLL motif and mediates the functional recycling of TrkA to the plasma membrane. We find that GGA3 depletion by siRNA delays TrkA recycling, accelerates TrkA degradation, attenuates sustained NGF-induced Akt activation, and reduces cell survival. We also show that GGA3’s effect on TrkA recycling is dependent on the activation of Arf6. This work identifies GGA3 as a key player in a novel DXXLL-mediated endosomal sorting machinery that targets TrkA to the plasma membrane, where it prolongs the activation of Akt signaling and survival responses. PMID:26446845

Uncertainties in s-process nucleosynthesis in massive stars determined by Monte Carlo variations

NASA Astrophysics Data System (ADS)

Nishimura, N.; Hirschi, R.; Rauscher, T.; St. J. Murphy, A.; Cescutti, G.

2017-08-01

The s-process in massive stars produces the weak component of the s-process (nuclei up to A ˜ 90), in amounts that match solar abundances. For heavier isotopes, such as barium, production through neutron capture is significantly enhanced in very metal-poor stars with fast rotation. However, detailed theoretical predictions for the resulting final s-process abundances have important uncertainties caused both by the underlying uncertainties in the nuclear physics (principally neutron-capture reaction and β-decay rates) as well as by the stellar evolution modelling. In this work, we investigated the impact of nuclear-physics uncertainties relevant to the s-process in massive stars. Using a Monte Carlo based approach, we performed extensive nuclear reaction network calculations that include newly evaluated upper and lower limits for the individual temperature-dependent reaction rates. We found that most of the uncertainty in the final abundances is caused by uncertainties in the neutron-capture rates, while β-decay rate uncertainties affect only a few nuclei near s-process branchings. The s-process in rotating metal-poor stars shows quantitatively different uncertainties and key reactions, although the qualitative characteristics are similar. We confirmed that our results do not significantly change at different metallicities for fast rotating massive stars in the very low metallicity regime. We highlight which of the identified key reactions are realistic candidates for improved measurement by future experiments.

Eruptive style and construction of shallow marine mafic tuff cones in the Narakay Volcanic Complex (Proterozoic, Hornby Bay Group, Northwest Territories, Canada)

NASA Astrophysics Data System (ADS)

Ross, Gerald M.

1986-03-01

The Early Proterozoic (1663 Ma) Narakay Volcanic Complex, exposed in Great Bear Lake (Northwest Territories, Canada), is a bimodal suite of basalt and rhyolite erupted in a continental setting and consisting largely of pyroclastic rocks interlayered with shallow marine sedimentary rocks of the Hornby Bay Group. Mafic pyroclastic rocks consist of lapilli tuff, tuff, tuff breccia and agglomerate that represent the remnants of small subaerial tuff cones (0.5 to 2 km in diameter) that in most cases have subsided into the volcanic conduit. Stratification styles, sedimentary structures and grain morphologies in pyroclastic rocks reflect variations in the water:magma ratio during eruptions and have been used to help elucidate eruptive mechanisms and reconstruct volcanic edifices. Basaltic pyroclasts are commonly bounded by fracture surfaces and are morphologically similar to modern pyroclasts produced by thermal quench fragmentation or steam-blast disruption of magma. Most fragments have low vesicularity and scoria is only locally abundant which indicates that eruptive energy was supplied mostly by water—melt interaction rather than exsolution of magmatic gases. Cored bombs and lapilli, fusiform bombs, and pyroclasts similar in texture to those of Strombolian cinder and agglutinate spatter, are uncommon but are stratigraphically widespread and imply the occurrence of Strombolian eruptions, presumably when water access to the vent was impeded. Massive bedding is typical of the tuffs and, in addition to the poorly sorted ash-rich nature of the tuffs, implies deposition from water- and/or steam-rich hydrovolcanic eruption clouds and cypressoid jets by airfall and dense pyroclastic flows. Uncommon well-stratified and sorted ash and lapilli tuff record airfall and pyroclastic flow(?) deposition from eruption clouds rich in magmatic gases. Base surge deposits are uncommon and occur only in the subaerial portion of a sequence of tuffs inferred to record the progradation of a cone-margin surge platform into standing water. Few of the tuff cone deposits display a systematic vertical sequence of stratification styles, structures and grain morphologies. This indicates that either the eruptive style varied irregularly between hydrovolcanic and Strombolian and/or that pyroclasts of different origin were mixed during eruptions.

Runaways: A Review of the Literature.

ERIC Educational Resources Information Center

Taylor, Jennifer; Mosteller, Frederick

More than half of runaway adolescents cite poor family communication and conflict as the primary reasons for running. Runaways (.5-1.5 million annually) generally arrive on the streets with few survival skills and little money. They are often subject to abuse of various sorts, and many eventually resort to criminal activity or use drugs in efforts…

Supersonic gas streams enhance the formation of massive black holes in the early universe.

PubMed

Hirano, Shingo; Hosokawa, Takashi; Yoshida, Naoki; Kuiper, Rolf

2017-09-29

The origin of super-massive black holes in the early universe remains poorly understood. Gravitational collapse of a massive primordial gas cloud is a promising initial process, but theoretical studies have difficulty growing the black hole fast enough. We report numerical simulations of early black hole formation starting from realistic cosmological conditions. Supersonic gas motions left over from the Big Bang prevent early gas cloud formation until rapid gas condensation is triggered in a protogalactic halo. A protostar is formed in the dense, turbulent gas cloud, and it grows by sporadic mass accretion until it acquires 34,000 solar masses. The massive star ends its life with a catastrophic collapse to leave a black hole-a promising seed for the formation of a monstrous black hole. Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Assessment of Aeolis Palus stratigraphic relationships based on bench-forming strata in the Kylie and the Kimberley regions of Gale crater, Mars

NASA Astrophysics Data System (ADS)

Williams, Rebecca M. E.; Malin, Michael C.; Stack, Kathryn M.; Rubin, David M.

2018-07-01

The stratigraphic context of rock layers is a critical piece of information needed for accurate reconstruction of their geologic history. Although sedimentary rocks are widespread in Gale crater, efforts to deduce stratigraphic relationships of rocks were challenging early in the Mars Science Laboratory mission because vertical bedrock exposures were relatively rare along the first ∼3 km the rover traversed across Aeolis Palus. Potential insights into the three-dimensional configuration of rock layers were made once the rover passed Dingo Gap, especially in the informally-named Kylie and Kimberley regions. Here, the terrain exhibits low relief ( < 10 m) cliffs, some of which are continuous over lengths > 75 m. Curiosity Mastcam and Navcam images show that the cliffs are capped by resistant, bench-forming rock layers corresponding to two facies: a poorly sorted, weakly stratified pebble conglomerate, and a massive, dark-toned, vuggy sandstone. In places, the inclination of the topographic surface (northward ∼2° to 3°) is similar to the apparent dip of the underlying strata, suggesting the presence of dip slopes in an area inferred to be generally flat-lying, conformable rock units. Further, we assessed potential strata correlations via plane-fitting exercises and a regional comparison to other capping strata. We speculate that bench-forming strata in the study region could be part of a widespread package of draping strata (the Siccar Point group) that post-dates deposition and exhumation of the lower strata of Mount Sharp.

Real-Time Pattern Recognition - An Industrial Example

NASA Astrophysics Data System (ADS)

Fitton, Gary M.

1981-11-01

Rapid advancements in cost effective sensors and micro computers are now making practical the on-line implementation of pattern recognition based systems for a variety of industrial applications requiring high processing speeds. One major application area for real time pattern recognition is in the sorting of packaged/cartoned goods at high speed for automated warehousing and return goods cataloging. While there are many OCR and bar code readers available to perform these functions, it is often impractical to use such codes (package too small, adverse esthetics, poor print quality) and an approach which recognizes an item by its graphic content alone is desirable. This paper describes a specific application within the tobacco industry, that of sorting returned cigarette goods by brand and size.

Differences in performance and learning proficiency on the Wisconsin Card Sorting Test in schizophrenia: do they reflect distinct neurocognitive subtypes with distinct functional profiles?

PubMed

Kurtz, Matthew M; Wexler, Bruce E

2006-01-31

The aim of this study was two-fold: 1) to determine whether a priori subtyping of patients with schizophrenia based on both overall performance on a measure of executive-function, the Wisconsin Card Sorting Test (WCST), and ability to learn the task with expanded instruction, could be confirmed with other, independent measures of executive-function and learning, and, if so, 2) to determine whether these groups have different neurocognitive profiles and show differences in functional capacity. Fifty-four outpatients with schizophrenia were divided by WCST performance into three groups: intact executive-function (n=28), "good learner" (n=13), and "poor learner" (n=13) groups. These groups were then assessed with a comprehensive neurocognitive test battery and a performance-based measure of functional status, the UCSD Performance-based Skills Assessment (UPSA). The WCST-intact subgroup performed significantly better than other groups on a second measure of executive-function and in working memory, and speeded motor sequencing. Impaired WCST patients who benefited from expanded WCST instruction ("good learners") also showed better performance than patients who did not benefit from instruction ("poor-learners") on a second measure of learning, as well as on a measure of auditory divided attention. The intact WCST subgroup had greater functional capacity than either "strong" or "poor" learners. These subtypes may have implications for response to behavioral treatment interventions.

Proximal pyroclastic deposits from the 1989-1990 eruption of Redoubt Volcano, Alaska - stratigraphy, distribution, and physical characteristics

USGS Publications Warehouse

Gardner, C.A.; Neal, C.A.; Waitt, R.B.; Janda, R.J.

1994-01-01

More than 20 eruptive events during the 1989-1990 eruption of Redoubt Volcano emplaced a complex sequence of lithic pyroclastic-flow, -surge, -fall, ice-diamict, and lahar deposits mainly on the north side of the volcano. The deposits record the changing eruption dynamics from initial gas-rich vent-clearing explosions to episodic gas-poor lava-dome extrusions and failures. The repeated dome failures produced lithic pyroclastic flows that mixed with snow and glacial ice to generate lahars that were channelled off Drift glacier into the Drift River valley. Some of the dome failures occurred without precursory seismic warning and appeared to result solely from gravitational instability. Material from the disrupted lava domes avalanched down a steep, partly ice-filled canyon incised on the north flank of the volcano and came to rest on the heavily crevassed surface of the piedmont lobe of Drift glacier. Most dome-collapse events resulted in single, monolithologic, massive to reversely graded, medium- to coarse-grained, sandy pyroclastic-flow deposits containing abundant dense dome clasts. These deposits vary in thickness, grain size, and texture depending on distance from the vent and local topography; deposits are finer and better sorted down flow, thinner and finer on hummocks, and thicker and coarser where ponded in channels cut through the glacial ice. The initial vent-clearing explosions emplaced unusual deposits of glacial ice, snow, and rock in a frozen matrix on the north and south flanks of the volcano. Similar deposits were described at Nevado del Ruiz, Columbia and have probably been emplaced at other snow-and-ice-clad volcanoes, but poor preservation makes them difficult to recognize in the geologic record. In a like fashion, most deposits from the 1989-1990 eruption of Redoubt Volcano may be difficult to recognize and interpret in the future because they were emplaced in an environment where glacio-fluvial processes dominate and quickly obscure the primary depositional record. ?? 1994.

Our Vocational Training Can Guarantee You the Job of a Lifetime. Consumer Bulletin No. 13.

ERIC Educational Resources Information Center

Federal Trade Commission, Washington, DC. Bureau of Consumer Protection.

This guidebook cautions the potential vocational school student about the possibilities of false claims, poor training, and unreliable job promises from commercial trade, technical business, and correspondence schools. It points out what sort of things to look for and which claims to take seriously. Defenses against an aggressive sales pitch are…

CHILDREN AND POVERTY.

ERIC Educational Resources Information Center

WITMER, HELEN L.

THREE MAJOR QUESTIONS ARE RAISED--(1) WHAT IS MEANT BY POVERTY AND TO WHAT EXTENT DOES THE OVERALL AMOUNT OF POVERTY DEPEND ON THE SORT OF MEASURING ROD USED. (2) HOW MANY AND WHAT PROPORTION OF THE NATION'S CHILDREN ARE GROWING UP IN POVERTY. AND (3) WHERE, GEOGRAPHICALLY AND SOCIALLY, ARE THESE CHILDREN OF THE POOR TO BE FOUND. POVERTY IS…

Flow cytometry sorting of nuclei enables the first global characterization of Paramecium germline DNA and transposable elements.

PubMed

Guérin, Frédéric; Arnaiz, Olivier; Boggetto, Nicole; Denby Wilkes, Cyril; Meyer, Eric; Sperling, Linda; Duharcourt, Sandra

2017-04-26

DNA elimination is developmentally programmed in a wide variety of eukaryotes, including unicellular ciliates, and leads to the generation of distinct germline and somatic genomes. The ciliate Paramecium tetraurelia harbors two types of nuclei with different functions and genome structures. The transcriptionally inactive micronucleus contains the complete germline genome, while the somatic macronucleus contains a reduced genome streamlined for gene expression. During development of the somatic macronucleus, the germline genome undergoes massive and reproducible DNA elimination events. Availability of both the somatic and germline genomes is essential to examine the genome changes that occur during programmed DNA elimination and ultimately decipher the mechanisms underlying the specific removal of germline-limited sequences. We developed a novel experimental approach that uses flow cell imaging and flow cytometry to sort subpopulations of nuclei to high purity. We sorted vegetative micronuclei and macronuclei during development of P. tetraurelia. We validated the method by flow cell imaging and by high throughput DNA sequencing. Our work establishes the proof of principle that developing somatic macronuclei can be sorted from a complex biological sample to high purity based on their size, shape and DNA content. This method enabled us to sequence, for the first time, the germline DNA from pure micronuclei and to identify novel transposable elements. Sequencing the germline DNA confirms that the Pgm domesticated transposase is required for the excision of all ~45,000 Internal Eliminated Sequences. Comparison of the germline DNA and unrearranged DNA obtained from PGM-silenced cells reveals that the latter does not provide a faithful representation of the germline genome. We developed a flow cytometry-based method to purify P. tetraurelia nuclei to high purity and provided quality control with flow cell imaging and high throughput DNA sequencing. We identified 61 germline transposable elements including the first Paramecium retrotransposons. This approach paves the way to sequence the germline genomes of P. aurelia sibling species for future comparative genomic studies.

Multi-wavelength investigations on feedback of massive star formation

NASA Astrophysics Data System (ADS)

Yuan, Jinghua

2014-05-01

In the course of massive star formation, outflows, ionizing radiation and intense stellar winds could heavily affect their adjacent environs and natal clouds. There are several outstanding open questions related to these processes: i) whether they can drive turbulence in molecular clouds; ii) whether they are able to trigger star formation; iii) whether they can destroy natal clouds to terminate star formation at low efficiencies. This thesis investigates feedback in different stages of massive star formation. Influence of such feedback to the ambient medium has been revealed. A new type of millimeter methanol maser is detected for the first time. An uncommon bipolar outflow prominent in the mid-infrared is discovered. And features of triggered star formation are found on the border of an infrared bubble and in the surroundings of a Herbig Be star. Extended green objects (EGOs) are massive outflow candidates showing prominent shocked features in the mid-infrared. We have carried out a high resolution study of the EGO G22.04+0.22 (hereafter, G22) based on archived SMA data. Continuum and molecular lines at 1.3 mm reveal that G22 is still at a hot molecular core stage. A very young multi-polar outflow system is detected, which is interacting with the adjacent dense gas. Anomalous emission features from CH3OH (8,-1,8 - 7,0,7) and CH3OH (4,2,2 - 3,1,2) are proven to be millimeter masers. It is the first time that maser emission of CH3OH (8,-1,8 - 7,0,7) at 218.440 GHz is detected in a massive star-forming region. Bipolar outflows have been revealed and investigated almost always in the microwave or radio domain. It's sort of rare that hourglass-shaped morphology be discovered in the mid-infrared. Based on GLIMPSE data, we have discovered a bipolar object resembling an hourglass at 8.0 um. It is found to be associated with IRAS 18114-1825. Analysis based on fitted SED, optical spectroscopy, and infrared color indices suggests IRAS 18114-1825 is an uncommon bipolar outflow driven by a massive protostar. Multi-wavelength observations based on classical tracers of outflows are highly necessary. Extensive investigations of IRAS 18114-1825 may contribute to our understanding of massive star formation in early stage.

Multi-locus phylogenetics, lineage sorting, and reticulation in Pinus subsection Australes.

PubMed

Gernandt, David S; Aguirre Dugua, Xitlali; Vázquez-Lobo, Alejandra; Willyard, Ann; Moreno Letelier, Alejandra; Pérez de la Rosa, Jorge A; Piñero, Daniel; Liston, Aaron

2018-04-23

Both incomplete lineage sorting and reticulation have been proposed as causes of phylogenetic incongruence. Disentangling these factors may be most difficult in long-lived, wind-pollinated plants with large population sizes and weak reproductive barriers. We used solution hybridization for targeted enrichment and massive parallel sequencing to characterize low-copy-number nuclear genes and high-copy-number plastomes (Hyb-Seq) in 74 individuals of Pinus subsection Australes, a group of ~30 New World pine species of exceptional ecological and economic importance. We inferred relationships using methods that account for both incomplete lineage sorting and reticulation. Concatenation- and coalescent-based trees inferred from nuclear genes mainly agreed with one another, but they contradicted the plastid DNA tree in recovering the Attenuatae (the California closed-cone pines) and Oocarpae (the egg-cone pines of Mexico and Central America) as monophyletic and the Australes sensu stricto (the southern yellow pines) as paraphyletic to the Oocarpae. The plastid tree featured some relationships that were discordant with morphological and geographic evidence and species limits. Incorporating gene flow into the coalescent analyses better fit the data, but evidence supporting the hypothesis that hybridization explains the non-monophyly of the Attenuatae in the plastid tree was equivocal. Our analyses document cytonuclear discordance in Pinus subsection Australes. We attribute this discordance to ancient and recent introgression and present a phylogenetic hypothesis in which mostly hierarchical relationships are overlain by gene flow. © 2018 The Authors. American Journal of Botany is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.

Granulometric analysis at Lampulo Fishing Port (LFP) substrate, Banda Aceh, Indonesia

NASA Astrophysics Data System (ADS)

Purnawan, S.; Setiawan, I.; Haridhi, H. A.; Irham, M.

2018-01-01

The study of sediment granulometry was completed at Lampulo fishing port (LFP). The LFP is a main fishing port in Aceh Province, Indonesia, located at 5°34’35” N; 95°19’23” E. The purpose of the research is to study and construct the environment condition of the bottom substrate. The data was taken by incorporating coring method at 10 stations using purposive random sampling. The wet sieve method was used to analyze the grain size for geostatistical analysis. The geostatistical parameters analysis in this study is classified as mean, sorting, skewness and kurtosis. The result informs that the types of sediments are sand, sandy clay and clayey sand for all stations. Station 1, however, is found as the coarsest compares to the other stations. All of the sediment collected at each station displays moderately sorted to poor sorted, while kurtosis values may be categorized as very leptokurtic. The results of the sediment parameters indicate that the environment of harbor pool was in a stable state, related to a sheltered condition.

Why the Arts Matter to Gifted Learners

ERIC Educational Resources Information Center

Smutny, Joan Franklin

2010-01-01

In the 1970s, Jose Antonio Abreu started the Venezuelan program that puts instruments into the hands of poor children and transforms their lives with free music education. Abreu, a musician and economist, sees in the arts not just a discipline and skill but a path to true selfhood. This is not the sort of talk one often finds in education.…

Still Red and in War or Just Poor? European Transformation Societies in Geography Textbooks

ERIC Educational Resources Information Center

Bagoly-Simo, Peter

2013-01-01

Textbooks are an important tool for teaching and learning in high school geography classrooms. But what sort of picture do they provide of places that are different and distant from the countries in which they are published? This study explores this question through a concise analysis of a widely used American world geography textbook, with a…

The Bolocam Galactic Plane Survey. XIV. Physical Properties of Massive Starless and Star-forming Clumps

NASA Astrophysics Data System (ADS)

Svoboda, Brian E.; Shirley, Yancy L.; Battersby, Cara; Rosolowsky, Erik W.; Ginsburg, Adam G.; Ellsworth-Bowers, Timothy P.; Pestalozzi, Michele R.; Dunham, Miranda K.; Evans, Neal J., II; Bally, John; Glenn, Jason

2016-05-01

We sort 4683 molecular clouds between 10° < ℓ < 65° from the Bolocam Galactic Plane Survey based on observational diagnostics of star formation activity: compact 70 μm sources, mid-IR color-selected YSOs, H2O and CH3OH masers, and UCH II regions. We also present a combined NH3-derived gas kinetic temperature and H2O maser catalog for 1788 clumps from our own GBT 100 m observations and from the literature. We identify a subsample of 2223 (47.5%) starless clump candidates (SCCs), the largest and most robust sample identified from a blind survey to date. Distributions of flux density, flux concentration, solid angle, kinetic temperature, column density, radius, and mass show strong (>1 dex) progressions when sorted by star formation indicator. The median SCC is marginally subvirial (α ˜ 0.7) with >75% of clumps with known distance being gravitationally bound (α < 2). These samples show a statistically significant increase in the median clump mass of ΔM ˜ 170-370 M ⊙ from the starless candidates to clumps associated with protostars. This trend could be due to (I) mass growth of the clumps at \\dot{M}˜ 200{--}440 M ⊙ Myr-1 for an average freefall 0.8 Myr timescale, (II) a systematic factor of two increase in dust opacity from starless to protostellar phases, and/or (III) a variation in the ratio of starless to protostellar clump lifetime that scales as ˜M -0.4. By comparing to the observed number of CH3OH maser containing clumps, we estimate the phase lifetime of massive (M > 103 M ⊙) starless clumps to be 0.37 ± 0.08 Myr (M/103 M ⊙)-1 the majority (M < 450 M ⊙) have phase lifetimes longer than their average freefall time.

The Enhancement of Bone Allograft Incorporation by the Local Delivery of the Sphingosine 1-phosphate Receptor Targeted Drug FTY720

PubMed Central

Aronin, Caren E Petrie; Shin, Soo J; Naden, Kimberly B; Rios, Peter D; Sefcik, Lauren S; Zawodny, Sarah R; Bagayoko, Namory D; Cui, Quanjun; Khan, Yusuf

2010-01-01

Poor vascularization coupled with mechanical instability is the leading cause of post-operative complications and poor functional prognosis of massive bone allografts. To address this limitation, we designed a novel continuous polymer coating system to provide sustained localized delivery of pharmacological agent, FTY720, a selective agonist for sphingosine 1-phosphate receptors, within massive tibial defects. In vitro drug release studies validated 64% loading efficiency with complete release of compound following 14 days. Mechanical evaluation following six weeks of healing suggested significant enhancement of mechanical stability in FTY720 treatment groups compared with unloaded controls. Furthermore, superior osseous integration across the host-graft interface, significant enhancement in smooth muscle cell investment, and reduction in leukocyte recruitment was evident in FTY720 treated groups compared with untreated groups. Using this approach, we can capitalize on the existing mechanical and biomaterial properties of devitalized bone, add a controllable delivery system while maintaining overall porous structure, and deliver a small molecule compound to constitutively target vascular remodeling, osseous remodeling, and minimize fibrous encapsulation within the allograft-host bone interface. Such results support continued evaluation of drug-eluting allografts as a viable strategy to improve functional outcome and long-term success of massive cortical allograft implants. PMID:20621764

Effects of sediment-associated extractable metals, degree of sediment grain sorting, and dissolved organic carbon upon Cryptosporidium parvum removal and transport within riverbank filtration sediments, Sonoma County, California.

PubMed

Metge, David W; Harvey, Ronald W; Aiken, George R; Anders, Robert; Lincoln, George; Jasperse, Jay; Hill, Mary C

2011-07-01

Oocysts of the protozoan pathogen Cryptosporidium parvum are of particular concern for riverbank filtration (RBF) operations because of their persistence, ubiquity, and resistance to chlorine disinfection. At the Russian River RBF site (Sonoma County, CA), transport of C. parvum oocysts and oocyst-sized (3 μm) carboxylate-modified microspheres through poorly sorted (sorting indices, σ(1), up to 3.0) and geochemically heterogeneous sediments collected between 2 and 25 m below land surface (bls) were assessed. Removal was highly sensitive to variations in both the quantity of extractable metals (mainly Fe and Al) and degree of grain sorting. In flow-through columns, there was a log-linear relationship (r(2) = 0.82 at p < 0.002) between collision efficiency (α, the probability that colloidal collisions with grain surfaces would result in attachment) and extractable metals, and a linear relationship (r(2) = 0.99 at p < 0.002) between α and σ(1). Collectively, variability in extractable metals and grain sorting accounted for ∼83% of the variability in α (at p < 0.0002) along the depth profiles. Amendments of 2.2 mg L(-1) of Russian River dissolved organic carbon (DOC) reduced α for oocysts by 4-5 fold. The highly reactive hydrophobic organic acid (HPOA) fraction was particularly effective in re-entraining sediment-attached microspheres. However, the transport-enhancing effects of the riverine DOC did not appear to penetrate very deeply into the underlying sediments, judging from high α values (∼1.0) observed for oocysts being advected through unamended sediments collected at ∼2 m bls. This study suggests that in evaluating the efficacy of RBF operations to remove oocysts, it may be necessary to consider not only the geochemical nature and size distribution of the sediment grains, but also the degrees of sediment sorting and the concentration, reactivity, and penetration of the source water DOC.

Pseudogynecomastia after massive weight loss: detectability of technique, patient satisfaction, and classification.

PubMed

Gusenoff, Jeffrey A; Coon, Devin; Rubin, J Peter

2008-11-01

An increasing number of male patients are presenting for treatment of male chest deformity after massive weight loss. The authors prefer to preserve the nipple-areola complex on a dermoglandular pedicle. They sought to identify detectability of technique, assess patient satisfaction, and outline a treatment algorithm for this population. Ten male massive weight loss patients underwent chest-contouring procedures over a period of 6 years and were surveyed to identify satisfaction with reconstruction. Preoperative photographs were used to devise a classification system. Twenty-seven medical professionals evaluated and rated digital photographs of the patients. Eight patients had pedicled reconstructions and two had free-nipple grafts. Mean age was 42.9 +/- 9.5 years, mean pre-weight loss body mass index was 54.1 +/- 10.6, post-weight loss body mass index was 29.4 +/- 4.5, and mean change in body mass index was 24.8 +/- 9.7. All patients would have surgery again, nine would recommend it to a friend, six would go shirtless in public, nine reported no loss of nipple sensation, and three reported dysesthesias of the nipple-areola complex. Medical professionals reproducibly associated poor wound healing with free-nipple grafting and rated poorly positioned nipple-areola complexes with low aesthetic scores. Medical professional scores for chest contour and nipple-areola complex aesthetics did not correlate with technique and were lower than scores provided by the patients. Patient satisfaction for treatment of the male chest deformity after massive weight loss is high. In carefully selected patients, preservation of the nipple-areola complex on a dermoglandular pedicle can aid in achieving an optimal aesthetic result.

Ages and Heavy Element Abundances from Very Metal-poor Stars in the Sagittarius Dwarf Galaxy

NASA Astrophysics Data System (ADS)

Hansen, Camilla Juul; El-Souri, Mariam; Monaco, Lorenzo; Villanova, Sandro; Bonifacio, Piercarlo; Caffau, Elisabetta; Sbordone, Luca

2018-03-01

Sagittarius (Sgr) is a massive disrupted dwarf spheroidal galaxy in the Milky Way halo that has undergone several stripping events. Previous chemical studies were restricted mainly to a few, metal-rich ([Fe/H] \\gtrapprox -1) stars that suggested a top-light initial mass function (IMF). Here we present the first high-resolution, very metal-poor ([Fe/H] =‑1 to ‑3) sample of 13 giant stars in the main body of Sgr. We derive abundances of 13 elements, namely C, Ca, Co, Fe, Sr, Ba, La, Ce, Nd, Eu, Dy, Pb, and Th, that challenge the interpretation based on previous studies. Our abundances from Sgr mimic those of the metal-poor halo, and our most metal-poor star ([Fe/H] ∼ -3) indicates a pure r-process pollution. Abundances of Sr, Pb, and Th are presented for the first time in Sgr, allowing for age determination using nuclear cosmochronology. We calculate ages of 9+/- 2.5 {Gyr}. Most of the sample stars have been enriched by a range of asymptotic giant branch (AGB) stars with masses between 1.3 and 5 M ⊙. Sgr J190651.47–320147.23 shows a large overabundance of Pb (2.05 dex) and a peculiar abundance pattern best fit by a 3 M ⊙ AGB star. Based on star-to-star scatter and observed abundance patterns, a mixture of low- and high-mass AGB stars and supernovae (15–25 M ⊙) is necessary to explain these patterns. The high level (0.29 ± 0.05 dex) of Ca indicates that massive supernovae must have existed and polluted the early ISM of Sgr before it lost its gas. This result is in contrast with a top-light IMF with no massive stars polluting Sgr. Based on data obtained UVES/VLT ID: 083.B-0774, 075.B-0127.

Rock Physics and Petrographic Parameters Relationship Within Siliciclastic Rocks: Quartz Sandstone Outcrop Study Case

NASA Astrophysics Data System (ADS)

Syafriyono, S.; Caesario, D.; Swastika, A.; Adlan, Q.; Syafri, I.; Abdurrokhim, A.; Mardiana, U.; Mohamad, F.; Alfadli, M. K.; Sari, V. M.

2018-03-01

Rock physical parameters value (Vp and Vs) is one of fundamental aspects in reservoir characterization as a tool to detect rock heterogenity. Its response is depend on several reservoir conditions such as lithology, pressure and reservoir fluids. The value of Vp and Vs is controlled by grain contact and contact stiffness, a function of clay mineral content and porosity also affected by mineral composition. The study about Vp and Vs response within sandstone and its relationship with petrographic parameters has become important to define anisotrophy of reservoir characteristics distribution and could give a better understanding about local diagenesis that influence clastic reservoir properties. Petrographic analysis and Vp-Vs calculation was carried out to 12 core sample which is obtained by hand-drilling of the outcrop in Sukabumi area, West Java as a part of Bayah Formation. Data processing and interpretation of sedimentary vertical succession showing that this outcrop comprises of 3 major sandstone layers indicating fluvial depositional environment. As stated before, there are 4 petrographic parameters (sorting, roundness, clay mineral content, and grain contact) which are responsible to the differences of shear wave and compressional wave value in this outcrop. Lithology with poor-sorted and well- roundness has Vp value lower than well-sorted and poor-roundness (sub-angular) grain. For the sample with high clay content, Vp value is ranging from 1681 to 2000 m/s and could be getting high until 2190 to 2714 m/s in low clay content sample even though the presence of clay minerals cannot be defined neither as matrix nor cement. The whole sample have suture grain contact indicating telogenesis regime whereas facies has no relationship with Vp and Vs value because of the different type of facies show similar petrographic parameters after diagenesis.

Chemistry of the Materials Above and Below an Unconformity Between the Murray and Stimson Formations in Gale Crater, Mars

NASA Astrophysics Data System (ADS)

Newsom, H. E.; Belgacem, I.; Wiens, R. C.; Frydenvang, J.; Gasnault, O.; Maurice, S.; Gasda, P. J.; Clegg, S. M.; Cousin, A.; Rapin, W.; Jackson, R.; Vaci, Z.; Ha, B.; Blaney, D. L.; Bridges, N.; Francis, R.; Payré, V.; Gupta, S.; Banham, S.; Schroeder, J.; Calef, F. J., III; Edgett, K. S.; Fey, D.; Fisk, M. R.; Gellert, R.; Thompson, L. M.; Perrett, G. M.; Grotzinger, J. P.; Rubin, D. M.; Williams, A.; Kah, L. C.; Kronyak, R. E.

2015-12-01

MSL began investigating a contact between Murray formation, (fine grained lake deposits) and the younger Stimson formation at Marias Pass in May 2015, on the lower slopes of Mt. Sharp. Images show that the Murray formation, with numerous calcium sulfate veins compared to the Stimson, is truncated at an erosional contact. MAHLI images show a white layer a few mm thick at the contact that might be calcium sulfate. The lowermost beds of the Stimson unit in the Missoula area comprise horizontally laminated or cross-laminated sandstones. The sandstones are poorly sorted with floating granules and very coarse sand grains set in a fine- medium-grained sand 'matrix'. This material directly above the contact is a resistant, basal ledge-forming layer that also forms numerous blocks of float on top of the eroded Murray. This basal layer contains light toned fragments, possibly calcium sulfate, eroded from the Murray. The poor sorting and presence of sub-angular grains, together with the absence of preferential sorting into size sorted layers would seem to rule out eolian processes for the lowermost beds of the Stimson and suggest fluvial processes were responsible for deposition of these beds. For chemostratigraphy, the distance of each ChemCam or APXS observation above or below the contact was determined from images and the NavCam stereo mesh. The top of the Murray near the Missoula area is variable in composition, and additional analyses are planned to determine if weathering occurred at the eroded surface. Above the contact, the lowest 2 cm of the resistant slab is higher in SiO2, and lower in Al2O3, K2O and Na2O, relative to other Stimson analyses. In a few points with low totals, there is a correlation between Ca and missing components (presumed to be mostly S). These points could be connected to calcium sulfate in the form of cements and/or incorporation of eroded clasts of Murray vein materials.

The Stopit! programme to reduce bullying and undermining behaviour in hospitals.

PubMed

Benmore, Graham; Henderson, Steven; Mountfield, Joanna; Wink, Brian

2018-05-21

Purpose The impact of bullying and undermining behaviours on the National Health Service on costs, patient safety and retention of staff was well understood even before the Illing report, published in 2013, that reviewed the efficacy of training interventions designed to reduce bullying and harassment in the outputs. The purpose of this paper is to provide an example of a good programme well evaluated. Design/methodology/approach The methodology follows a broad realist approach, by specifying the underlying programme assumptions and intention of the designers. Three months after the event, Q-sort methodology was employed to group participants into one of three contexts - mechanism - output groups. Interviews were then undertaken with members of two of these groups, to evaluate how the programme had influenced each. Findings Q-sort identified a typology of three beneficiaries from the Stopit! workshops, characterised as professionals, colleagues and victims. Each group had acted upon different parts of the programme, depending chiefly upon their current and past experiences of bullying in hospitals. Research limitations/implications The paper demonstrates the effectiveness of using Q-sort method to identify relevant CMOs in a realist evaluation framework. Practical implications The paper considers the effectiveness of the programme to reduce bullying, rather than teach victims to cope, and how it may be strengthened based upon the research findings and Illing recommendations. Social implications Workplace bullying is invariably implicated in scandals concerning poor hospital practice, poor patient outcomes and staff illness. All too frequently, the sector responds by offering training in resilience, which though helpful, places the onus on the victim to cope rather than the employer to reduce or eliminate the practice. This paper documents and evaluates an attempt to change workplace practices to directly address bullying and undermining. Originality/value The paper describes a new programme broadly consistent with Illing report endorsements. Second, it illustrates a novel evaluation method that highlights rigorously the contexts, mechanisms and outcomes at the pilot stage of an intervention identifies contexts and mechanisms via factor analysis using Q-sort methodology.

Flows of X-ray gas reveal the disruption of a star by a massive black hole.

PubMed

Miller, Jon M; Kaastra, Jelle S; Miller, M Coleman; Reynolds, Mark T; Brown, Gregory; Cenko, S Bradley; Drake, Jeremy J; Gezari, Suvi; Guillochon, James; Gultekin, Kayhan; Irwin, Jimmy; Levan, Andrew; Maitra, Dipankar; Maksym, W Peter; Mushotzky, Richard; O'Brien, Paul; Paerels, Frits; de Plaa, Jelle; Ramirez-Ruiz, Enrico; Strohmayer, Tod; Tanvir, Nial

2015-10-22

Tidal forces close to massive black holes can violently disrupt stars that make a close approach. These extreme events are discovered via bright X-ray and optical/ultraviolet flares in galactic centres. Prior studies based on modelling decaying flux trends have been able to estimate broad properties, such as the mass accretion rate. Here we report the detection of flows of hot, ionized gas in high-resolution X-ray spectra of a nearby tidal disruption event, ASASSN-14li in the galaxy PGC 043234. Variability within the absorption-dominated spectra indicates that the gas is relatively close to the black hole. Narrow linewidths indicate that the gas does not stretch over a large range of radii, giving a low volume filling factor. Modest outflow speeds of a few hundred kilometres per second are observed; these are below the escape speed from the radius set by variability. The gas flow is consistent with a rotating wind from the inner, super-Eddington region of a nascent accretion disk, or with a filament of disrupted stellar gas near to the apocentre of an elliptical orbit. Flows of this sort are predicted by fundamental analytical theory and more recent numerical simulations.

Winds of metal-poor OB stars: Updates from HST-COS UV spectroscopy

NASA Astrophysics Data System (ADS)

García, M.; Herrero, A.; Najarro, F.; Lennon, D. J.; Urbaneja, M. A.

2015-01-01

In the race to break the SMC frontier and reach metallicity conditions closer to the First Stars the information from UV spectroscopy is usually overlooked. New HST-COS observations of OB stars in the metal-poor galaxy IC1613, with oxygen content ~1/10 solar, have proved the important role of UV spectroscopy to characterize blue massive stars and their winds. The terminal velocities (υ∞) and abundances derived from the dataset have shed new light on the problem of metal-poor massive stars with strong winds. Furthermore, our results question the υ∞-υ esc and υ∞-Z scaling relations whose use in optical-only studies may introduce large uncertainties in the derived mass loss rates and wind-momenta. Finally, our results indicate that the detailed abundance pattern of each star may have a non-negligible impact on its wind properties, and scaling these as a function of one single metallicity parameter is probably too coarse an approximation. Considering, for instance, that the [α/Fe] ratio evolves with the star formation history of each galaxy, we may be in need of updating all our wind recipes.

Assessing the role of detrital zircon sorting on provenance interpretations in an ancient fluvial system using paleohydraulics - Permian Cutler Group, Paradox Basin, Utah and Colorado

NASA Astrophysics Data System (ADS)

Findlay, C. P., III; Ewing, R. C.; Perez, N. D.

2017-12-01

Detrital zircon age signatures used in provenance studies are assumed to be representative of entire catchments from which the sediment was derived, but the extent to which hydraulic sorting can bias provenance interpretations is poorly constrained. Sediment and mineral sorting occurs with changes in hydraulic conditions driven by both allogenic and autogenic processes. Zircon is sorted from less dense minerals due to the difference in density, and any age dependence on zircon size could potentially bias provenance interpretations. In this study, a coupled paleohydraulic and geochemical provenance approach is used to identify changes in paleohydraulic conditions and relate them to spatial variations in provenance signatures from samples collected along an approximately time-correlative source-to-sink pathway in the Permian Cutler Group of the Paradox Basin. Samples proximal to the uplift have a paleoflow direction to the southwest. In the medial basin, paleocurrent direction indicates salt movement caused fluvial pathways divert to the north and northwest on the flanks of anticlines. Channel depth, flow velocity, and discharge calculations were derived from field measurements of grain size and dune and bar cross-stratification indicate that competency of the fluvial system decreased from proximal to the medial basin by up to a factor of 12. Based upon the paleohydraulic calculations, zircon size fractionation would occur along the transect such that the larger zircons are removed from the system prior to reaching the medial basin. Analysis of the size and age distribution of zircons from the proximal and distal fluvial system of the Cutler Group tests if this hydraulic sorting affects the expected Uncompahgre Uplift age distribution.

A Peculiar Sentiment Analysis Advancement in Big Data

NASA Astrophysics Data System (ADS)

Valera, Manisha; Patel, Yash

2018-01-01

Every company wants to discover what its customers feel about it. But sentiment analysis can get coarser and turn inward to improve employee as well as customers’ satisfaction. A term called sentiment analysis, or the mathematical taxonomy of statements’ negative or positive connotations, gives companies potent ways to analyse cumulative language data across all sorts of communications. There’s real value in measuring sentiment inside and outside your company. Our big data experts analyse the core grounds of the problems raised while handling giant datasets and find solutions to efficiently manage massive datasets with ease. We built a sentiment analysis solution to measure positive and negative sentiments with top rating products for Amazon.com‘s Electronics products as against competitor products.

Information and Exit: Do Accountability Ratings Help Families Choose Schools? Program on Education Policy and Governance Working Papers Series. PEPG 09-06

ERIC Educational Resources Information Center

Henderson, Michael

2009-01-01

Does public information about school quality lead parents to sort their children out of schools with relatively poor performance? Use of this exit option in response to information about school quality has the potential to indirectly foster school responsiveness to quality concerns. To determine whether this information affects student exit, I…

Understanding Optimal Decision-Making in Wargaming III

DTIC Science & Technology

2015-10-01

attention - deficit / hyperactivity disorder assessed by the Keio version of the Wisconsin card sorting test. Brain and Development, 34(5), 354-359...immediate feedback regarding friendly, enemy, and total damage. In addition to latency response (LR), attention to feedback also was measured by...measures can detect poor attention allocation. This material is based upon work supported in part by the Army Research Office (62626- NS). The

The Narrowing Gap in New York City Teacher Qualifications and Its Implications for Student Achievement in High-Poverty Schools. Working Paper 10

ERIC Educational Resources Information Center

Boyd, Donald; Lankford, Hamilton; Loeb, Susanna; Rockoff, Jonah; Wyckoff, James

2007-01-01

Studies have found substantial sorting of teachers across schools, with the schools with the highest proportions of poor, non-white, and low-scoring students having the least qualified teachers as measured by certification, exam performance, and inexperience (Lankford, Loeb and Wyckoff, 2002). Yet, there have been substantial changes in the…

A Lidar-derived evaluation of watershed-scale large woody debris sources and recruitment mechanisms: costal Maine, USA

Treesearch

A. ​Kasprak; F. J. Magilligan; K. H. Nislow; N. P. Snyder

2012-01-01

In‐channel large woody debris (LWD) promotes quality aquatic habitat through sediment sorting, pool scouring and in‐stream nutrient retention and transport. LWD recruitment occurs by numerous ecological and geomorphic mechanisms including channel migration, mass wasting and natural tree fall, yet LWD sourcing on the watershed scale remains poorly constrained. We...

Multifunctional, inexpensive, and reusable nanoparticle-printed biochip for cell manipulation and diagnosis

PubMed Central

Esfandyarpour, Rahim; DiDonato, Matthew J.; Yang, Yuxin; Durmus, Naside Gozde; Harris, James S.; Davis, Ronald W.

2017-01-01

Isolation and characterization of rare cells and molecules from a heterogeneous population is of critical importance in diagnosis of common lethal diseases such as malaria, tuberculosis, HIV, and cancer. For the developing world, point-of-care (POC) diagnostics design must account for limited funds, modest public health infrastructure, and low power availability. To address these challenges, here we integrate microfluidics, electronics, and inkjet printing to build an ultra–low-cost, rapid, and miniaturized lab-on-a-chip (LOC) platform. This platform can perform label-free and rapid single-cell capture, efficient cellular manipulation, rare-cell isolation, selective analytical separation of biological species, sorting, concentration, positioning, enumeration, and characterization. The miniaturized format allows for small sample and reagent volumes. By keeping the electronics separate from microfluidic chips, the former can be reused and device lifetime is extended. Perhaps most notably, the device manufacturing is significantly less expensive, time-consuming, and complex than traditional LOC platforms, requiring only an inkjet printer rather than skilled personnel and clean-room facilities. Production only takes 20 min (vs. up to weeks) and $0.01—an unprecedented cost in clinical diagnostics. The platform works based on intrinsic physical characteristics of biomolecules (e.g., size and polarizability). We demonstrate biomedical applications and verify cell viability in our platform, whose multiplexing and integration of numerous steps and external analyses enhance its application in the clinic, including by nonspecialists. Through its massive cost reduction and usability we anticipate that our platform will enable greater access to diagnostic facilities in developed countries as well as POC diagnostics in resource-poor and developing countries. PMID:28167769

Correlating Structural Order with Structural Rearrangement in Dusty Plasma Liquids: Can Structural Rearrangement be Predicted by Static Structural Information?

NASA Astrophysics Data System (ADS)

Su, Yen-Shuo; Liu, Yu-Hsuan; I, Lin

2012-11-01

Whether the static microstructural order information is strongly correlated with the subsequent structural rearrangement (SR) and their predicting power for SR are investigated experimentally in the quenched dusty plasma liquid with microheterogeneities. The poor local structural order is found to be a good alarm to identify the soft spot and predict the short term SR. For the site with good structural order, the persistent time for sustaining the structural memory until SR has a large mean value but a broad distribution. The deviation of the local structural order from that averaged over nearest neighbors serves as a good second alarm to further sort out the short time SR sites. It has the similar sorting power to that using the temporal fluctuation of the local structural order over a small time interval.

The CGM of Massive Galaxies: Where Cold Gas Goes to Die?

NASA Astrophysics Data System (ADS)

Howk, Jay

2017-08-01

We propose to survey the cold HI content and metallicity of the circumgalactic medium (CGM) around 50 (45 new, 5 archival) z 0.5 Luminous Red Galaxies (LRGs) to directly test a fundamental prediction of galaxy assembly models: that cold, metal-poor accretion does not survive to the inner halos of very massive galaxies. Accretion and feedback through the CGM play key roles in our models of the star formation dichotomy in galaxies. Low mass galaxies are thought to accrete gas in cold streams, while high mass galaxies host hot, dense halos that heat incoming gas and prevent its cooling, thereby quenching star formation. HST/COS has provided evidence for cold, metal-poor streams in the halos of star-forming galaxies (consistent with cold accretion). Observations have also demonstrated the presence of cool gas in the halos of passive galaxies, a potential challenge to the cold/hot accretion model. Our proposed observations will target the most massive galaxies and address the origin of the cool CGM gas by measuring the metallicity. This experiment is enabled by our novel approach to deriving metallicities, allowing the use of much fainter QSOs. It cannot be done with archival data, as these rare systems are not often probed along random sight lines. The H I column density (and metallicity) measurements require access to the UV. The large size of our survey is crucial to robustly assess whether the CGM in these galaxies is unique from that of star-forming systems, a comparison that provides the most stringent test of cold-mode accretion/quenching models to date. Conversely, widespread detections of metal-poor gas in these halos will seriously challenge the prevailing theory.

Isolation and characterization of human mesenchymal stem cells derived from synovial fluid by magnetic-activated cell sorting (MACS).

PubMed

Jia, Zhaofeng; Liang, Yujie; Xu, Xiao; Li, Xingfu; Liu, Qisong; Ou, Yangkan; Duan, Li; Zhu, Weimin; Lu, Wei; Xiong, Jianyi; Wang, Daping

2018-03-01

Mesenchymal stem cells (MSCs) are the primary source of cells used for cell-based therapy in tissue engineering. MSCs are found in synovial fluid, a source that could be conveniently used for cartilage tissue engineering. However, the purification and characterization of SF-MSCs has been poorly documented in the literature. Here, we outline an easy-to-perform approach for the isolation and culture of MSCs derived from human synovial fluid (hSF-MSCs). We have successfully purified hSF-MSCs using magnetic-activated cell sorting (MACS) using the MSC surface marker, CD90. Purified SF-MSCs demonstrate significant renewal capacity following several passages in culture. Furthermore, we demonstrated that MACS-sorted CD90 + cells could differentiated into osteoblasts, adipocytes, and chondrocytes in vitro. In addition, we show that these cells can generate cartilage tissue in micromass culture as well. This study demonstrates that MACS is a useful tool that can be used for the purification of hSF-MSCs from synovial fluid. The proliferation properties and ability to differentiate into chondrocytes make these hSF-MSCs a promising source of stem cells for applications in cartilage repair. © 2017 International Federation for Cell Biology.

Pop III i-process nucleosynthesis and the elemental abundances of SMSS J0313-6708 and the most iron-poor stars

NASA Astrophysics Data System (ADS)

Clarkson, O.; Herwig, F.; Pignatari, M.

2018-02-01

We have investigated a highly energetic H-ingestion event during shell He burning leading to H-burning luminosities of log (LH/L⊙) ˜ 13 in a 45 M⊙ Pop III massive stellar model. In order to track the nucleosynthesis which may occur in such an event, we run a series of single-zone nucleosynthesis models for typical conditions found in the stellar evolution model. Such nucleosynthesis conditions may lead to i-process neutron densities of up to ˜1013 cm-3. The resulting simulation abundance pattern, where Mg comes from He burning and Ca from the i process, agrees with the general observed pattern of the most iron-poor star currently known, SMSS J031300.36-670839.3. However, Na is also efficiently produced in these i-process conditions, and the prediction exceeds observations by ˜2.5 dex. While this probably rules out this model for SMSS J031300.36-670839.3, the typical i-process signature of combined He burning and i process of higher than solar [Na/Mg], [Mg/Al], and low [Ca/Mg] is reproducing abundance features of the two next most iron-poor stars HE 1017-5240 and HE 1327-2326 very well. The i process does not reach Fe which would have to come from a low level of additional enrichment. i process in hyper-metal-poor or Pop III massive stars may be able to explain certain abundance patterns observed in some of the most metal-poor CEMP-no stars.

Identifying Children With Poor Cochlear Implantation Outcomes Using Massively Parallel Sequencing

PubMed Central

Wu, Chen-Chi; Lin, Yin-Hung; Liu, Tien-Chen; Lin, Kai-Nan; Yang, Wei-Shiung; Hsu, Chuan-Jen; Chen, Pei-Lung; Wu, Che-Ming

2015-01-01

Abstract Cochlear implantation is currently the treatment of choice for children with severe to profound hearing impairment. However, the outcomes with cochlear implants (CIs) vary significantly among recipients. The purpose of the present study is to identify the genetic determinants of poor CI outcomes. Twelve children with poor CI outcomes (the “cases”) and 30 “matched controls” with good CI outcomes were subjected to comprehensive genetic analyses using massively parallel sequencing, which targeted 129 known deafness genes. Audiological features, imaging findings, and auditory/speech performance with CIs were then correlated to the genetic diagnoses. We identified genetic variants which are associated with poor CI outcomes in 7 (58%) of the 12 cases; 4 cases had bi-allelic PCDH15 pathogenic mutations and 3 cases were homozygous for the DFNB59 p.G292R variant. Mutations in the WFS1, GJB3, ESRRB, LRTOMT, MYO3A, and POU3F4 genes were detected in 7 (23%) of the 30 matched controls. The allele frequencies of PCDH15 and DFNB59 variants were significantly higher in the cases than in the matched controls (both P < 0.001). In the 7 CI recipients with PCDH15 or DFNB59 variants, otoacoustic emissions were absent in both ears, and imaging findings were normal in all 7 implanted ears. PCDH15 or DFNB59 variants are associated with poor CI performance, yet children with PCDH15 or DFNB59 variants might show clinical features indistinguishable from those of other typical pediatric CI recipients. Accordingly, genetic examination is indicated in all CI candidates before operation. PMID:26166082

Winds of very low metallicity OB stars: crossing the frontier of the Magellanic Clouds

NASA Astrophysics Data System (ADS)

Garcia, Miriam

2011-10-01

Very low metallicity massive stars are a key ingredient for our understanding of the early Universe because of their connection with the dominant conditions at that time, the reionization epoch and long-GRBs. In the studies of massive stars radiation driven winds play a crucial manifold role, being a chief agent of stellar evolution, altering the optical diagnostics for parameter determination and injecting radiative and mechanical energy into their surroundings. However, the theory of radiation driven winds has only be tested down to SMC metallicities and some important open questions remain: the existence of solar-metallicity stars with weak winds and very recent evidence of relatively strong winds in metal-poor stars.We have secured VLT optical spectra of a sample of early-type massive stars in IC 1613, a very metal poor { <0.1Zo} irregular galaxy of the Local Group that represents the next step towards low metallicities after the SMC. We request low resolution COS spectra {COS/FUV-G140L} of a sub-set of OB stars probing different wind regimes. The wind lines in the 1150-1800A range, together with the optical spectra, will allow us to derive consistently the photospheric and wind parameters of the sample. Results will be interpreted in the context of both evolutionary and radiatively driven winds theories, testing the current paradigm at unexplored low metallicities and increasing our knowledge of massive stars under conditions closer to those of the deep Universe.COS enhanced sensitivity will allow us to perform for the first time detailed studies of **resolved** OB stars in an environment with poorer metal content than the SMC.

Does Information Help Families Choose Schools? Evidence from a Regression Discontinuity Design. Program on Education Policy and Governance Working Papers Series. PEPG 10-17

ERIC Educational Resources Information Center

Henderson, Michael

2010-01-01

Does public information about school quality lead parents to sort their children out of schools with relatively poor performance? Use of this exit option in response to information about school quality has the potential to indirectly foster school responsiveness to quality concerns. To determine whether this information affects student exit, I use…

Installation Restoration Program. Preliminary Assessment: Connecticut Air National Guard, 103rd Tactical Fighter Group (TFG), Bradley International Airport, Windsor Locks, Connecticut and 103rd Tactical Control Squadron (TCS), Orange/West Haven, Connectiut

DTIC Science & Technology

1988-11-01

poorly sorted, not I E compacted, very plastic . Contains siliceous N diatoms and spores. Organic content high (17.2 T percent of sample lost during...physical character of a rock (e.g., particle size, color, mineral content, primary strutures, thickness, weathering caracteristics , and other physical

Bode's Berlin Astronomisches Jahrbuch as a biographical source (German Title: Bodes Berliner Astronomisches Jahrbuch als biographische Quelle)

NASA Astrophysics Data System (ADS)

Schwemin, Friedhelm

2010-12-01

The appendices of the first 54 volumes (1776-1829) of the Berliner Astronomisches Jahrbuch (BAJ), edited by Johann Elert Bode, contain a plethora of biographically relevant notes, which are listed here, alphabetically sorted, in short versions. In parts, the listing possesses the quality of a primary source, and contains information on 771 persons. Many of them are poorly known or unknown.

Long-term outcomes of patients receiving a massive transfusion after trauma.

PubMed

Mitra, Biswadev; Gabbe, Belinda J; Kaukonen, Kirsi-Maija; Olaussen, Alexander; Cooper, David J; Cameron, Peter A

2014-10-01

Resuscitation of patients presenting with hemorrhagic shock after major trauma has evolved to incorporate multiple strategies to maintain tissue perfusion and oxygenation while managing coagulation disorders. We aimed to study changes across time in long-term outcomes in patients with major trauma. A retrospective observational study in a single major trauma center in Australia was conducted. We included all patients with major trauma and massive blood transfusion within the first 24 h during a 6-year period (from 2006 to 2011). The main outcome measures were Glasgow Outcome Score-Extended (GOSE) and work capacity at 6 and 12 months. There were 5,915 patients with major trauma of which 365 (6.2%; 95% confidence interval [95% CI], 5.6 - 6.8) received a massive transfusion. The proportion of major trauma patients receiving a massive transfusion decreased across time from 8.2% to 4.4% (P < 0.01). There were statistically significant trends toward lower volumes of red blood cell transfusion and higher ratios of fresh-frozen plasma to red blood cells (P < 0.01). Among massively transfused patients, there was no significant change in measured outcomes during the study period, with a persistent 23% mortality in hospital, 52% unfavorable GOSE at 6 months, and 44% unfavorable GOSE at 12 months. Massive transfusion was independently associated with unfavorable outcomes at 6 months after injury (adjusted odds ratio, 1.56; 95% CI, 1.05 - 2.31) but not at 12 months (adjusted odds ratio, 0.85; 95% CI, 0.72 - 1.01). A significant reduction in massive transfusion rates was observed. Unfavorable long-term outcomes among patients receiving a massive transfusion after trauma were frequent with a substantial proportion of survivors experiencing poor functional status 1 year after injury.

High Energy Interactions in Massive Binaries: An Application to a Most Mysterious Binary

NASA Technical Reports Server (NTRS)

Corcoran, Michael

2013-01-01

Extremely massive stars (50M and above) are exceedingly rare in the local Universe but are believed to have composed the entire first generation of stars, which lived fast, died young and left behind the first generation of black holes and set the stage for the formation of lower mass stars suitable to support life. There are significant uncertainties about how this happened (and how it still happens), mostly due to our poor knowledge of how stars change mass as they evolve. Extremely massive stars give mass back to the ISM via strong radiatively-driven winds and sometimes through sporadic eruptions of the most massive and brightest stars. Such mass loss plays an important role in the chemical and dynamical evolution of the local interstellar medium prior to the supernova explosion. Below we discuss how high energy thermal (and, in some cases, non-thermal) emission, along with modern simulations in 2 and 3 dimensions, can be used to help determine a physically realistic picture of mass loss in a well-studied, mysterious system.

Twin troubles--rickets causing myelofibrosis.

PubMed

Kamien, Benjamin; Harris, Linda

2007-01-01

Myelofibrosis is an uncommon condition that causes anaemia, failure to thrive and massive splenomegaly. This case report describes migrant Sudanese twins who developed myelofibrosis secondary to severe rickets from a combination of poor diet, inadequate sun exposure, and a breastfeeding mother who wore hijab and was also vitamin D deficient.

Debilitating lung disease among surface coal miners with no underground mining tenure.

PubMed

Halldin, Cara N; Reed, William R; Joy, Gerald J; Colinet, Jay F; Rider, James P; Petsonk, Edward L; Abraham, Jerrold L; Wolfe, Anita L; Storey, Eileen; Laney, A Scott

2015-01-01

To characterize exposure histories and respiratory disease among surface coal miners identified with progressive massive fibrosis from a 2010 to 2011 pneumoconiosis survey. Job history, tenure, and radiograph interpretations were verified. Previous radiographs were reviewed when available. Telephone follow-up sought additional work and medical history information. Among eight miners who worked as drill operators or blasters for most of their tenure (median, 35.5 years), two reported poor dust control practices, working in visible dust clouds as recently as 2012. Chest radiographs progressed to progressive massive fibrosis in as few as 11 years. One miner's lung biopsy demonstrated fibrosis and interstitial accumulation of macrophages containing abundant silica, aluminum silicate, and titanium dust particles. Overexposure to respirable silica resulted in progressive massive fibrosis among current surface coal miners with no underground mining tenure. Inadequate dust control during drilling/blasting is likely an important etiologic factor.

Debilitating Lung Disease Among Surface Coal Miners With No Underground Mining Tenure

PubMed Central

Halldin, Cara N.; Reed, William R.; Joy, Gerald J.; Colinet, Jay F.; Rider, James P.; Petsonk, Edward L.; Abraham, Jerrold L.; Wolfe, Anita L.; Storey, Eileen; Laney, A. Scott

2015-01-01

Objective To characterize exposure histories and respiratory disease among surface coal miners identified with progressive massive fibrosis from a 2010 to 2011 pneumoconiosis survey. Methods Job history, tenure, and radiograph interpretations were verified. Previous radiographs were reviewed when available. Telephone follow-up sought additional work and medical history information. Results Among eight miners who worked as drill operators or blasters for most of their tenure (median, 35.5 years), two reported poor dust control practices, working in visible dust clouds as recently as 2012. Chest radiographs progressed to progressive massive fibrosis in as few as 11 years. One miner’s lung biopsy demonstrated fibrosis and interstitial accumulation of macrophages containing abundant silica, aluminum silicate, and titanium dust particles. Conclusions Overexposure to respirable silica resulted in progressive massive fibrosis among current surface coal miners with no underground mining tenure. Inadequate dust control during drilling/blasting is likely an important etiologic factor. PMID:25563541

Hunting for Shooting Stars in 30 Doradus

NASA Astrophysics Data System (ADS)

de Mink, Selma E.; Lennon, D. J.; Sabbi, E.; Anderson, J.; Bedin, L. R.; Sohn, S.; van der Marel, R. P.; Walborn, N. R.; Bastian, N.; Bressert, E.; Crowther, P. A.; Evans, C. J.; Herrero, A.; Langer, N.; Sana, H.

2012-01-01

We are undertaking an ambitious proper motion survey of massive stars in the 30 Doradus region of the Large Magellanic Cloud using the unique capabilities of HST. We aim to derive the directions of motion of massive runaway stars, searching in particular for stars which have been ejected from the dense star cluster R136. These stars probe the dynamical processes in the core of the cluster. The core has been suggested as a formation site for very massive stars exceeding the canonical upper limit of the IMF. These are possible progenitors of intermediate-mass black holes. Furthermore, they provide insight about the origin of massive field stars, addressing open questions related to the poorly understood process of massive star formation. Some may originate from disrupted binary systems and bear the imprints of interaction with the original companion. They will end their life far away from their birth location as core collapse supernova or possibly even long gamma-ray bursts. Here we discuss the first epoch of observations, presenting a 16'x13' mosaic of the data, and initial results based on comparisons with archival data. SdM acknowledges the NASA Hubble Fellowship grant HST-HF-51270.01-A awarded by STScI, operated by AURA for NASA, contract NAS 5-26555.

The mass distribution of Population III stars

NASA Astrophysics Data System (ADS)

Fraser, M.; Casey, A. R.; Gilmore, G.; Heger, A.; Chan, C.

2017-06-01

Extremely metal-poor (EMP) stars are uniquely informative on the nature of massive Population III stars. Modulo a few elements that vary with stellar evolution, the present-day photospheric abundances observed in EMP stars are representative of their natal gas cloud composition. For this reason, the chemistry of EMP stars closely reflects the nucleosynthetic yields of supernovae from massive Population III stars. Here we collate detailed abundances of 53 EMP stars from the literature and infer the masses of their Population III progenitors. We fit a simple initial mass function (IMF) to a subset of 29 of the inferred Population III star masses, and find that the mass distribution is well represented by a power-law IMF with exponent α = 2.35^{+0.29}_{-0.24}. The inferred maximum progenitor mass for supernovae from massive Population III stars is M_{max} = 87^{+13}_{-33} M⊙, and we find no evidence in our sample for a contribution from stars with masses above ˜120 M⊙. The minimum mass is strongly consistent with the theoretical lower mass limit for Population III supernovae. We conclude that the IMF for massive Population III stars is consistent with the IMF of present-day massive stars and there may well have formed stars much below the supernova mass limit that could have survived to the present day.

Acute intoxication and recovery following massive turpentine ingestion: clinical and toxicological data.

PubMed

Troulakis, G; Tsatsakis, A M; Tzatzarakis, M; Astrakianakis, A; Dolapsakis, G; Kostas, R

1997-06-01

Reports of acute turpentine intoxication, particularly containing toxicological data, are poorly verified in the literature. This report regards the intentional massive ingestion of turpentine solution in an elderly woman who developed mainly central nervous system manifestations, then had an impressive and quick total recovery although the initial prognosis was very bad. Blood and urine levels of turpentine were monitored using gas chromatography and at the early toxicogenic stage were 28 micrograms/mL and 15 micrograms/mL respectively. Gastric fluid analysis on admission to the hospital revealed the presence of approximately 200 mL turpentine in the intestine. A review of earlier reports is given.

Mining Personal Data Using Smartphones and Wearable Devices: A Survey

PubMed Central

Rehman, Muhammad Habib ur; Liew, Chee Sun; Wah, Teh Ying; Shuja, Junaid; Daghighi, Babak

2015-01-01

The staggering growth in smartphone and wearable device use has led to a massive scale generation of personal (user-specific) data. To explore, analyze, and extract useful information and knowledge from the deluge of personal data, one has to leverage these devices as the data-mining platforms in ubiquitous, pervasive, and big data environments. This study presents the personal ecosystem where all computational resources, communication facilities, storage and knowledge management systems are available in user proximity. An extensive review on recent literature has been conducted and a detailed taxonomy is presented. The performance evaluation metrics and their empirical evidences are sorted out in this paper. Finally, we have highlighted some future research directions and potentially emerging application areas for personal data mining using smartphones and wearable devices. PMID:25688592

Effects of sediment-associated extractable metals, degree of sediment grain sorting, and dissolved organic carbon upon Cryptosporidium parvum removal and transport within riverbank filtration sediments, Sonoma County, California

USGS Publications Warehouse

Metge, D.W.; Harvey, R.W.; Aiken, G.R.; Anders, R.; Lincoln, G.; Jasperse, James; Hill, M.C.

2011-01-01

Oocysts of the protozoan pathogen Cryptosporidium parvum are of particular concern for riverbank filtration (RBF) operations because of their persistence, ubiquity, and resistance to chlorine disinfection. At the Russian River RBF site (Sonoma County, CA), transport of C. parvumoocysts and oocyst-sized (3 μm) carboxylate-modified microspheres through poorly sorted (sorting indices, σ1, up to 3.0) and geochemically heterogeneous sediments collected between 2 and 25 m below land surface (bls) were assessed. Removal was highly sensitive to variations in both the quantity of extractable metals (mainly Fe and Al) and degree of grain sorting. In flow-through columns, there was a log–linear relationship (r2 = 0.82 at p < 0.002) between collision efficiency (α, the probability that colloidal collisions with grain surfaces would result in attachment) and extractable metals, and a linear relationship (r2 = 0.99 at p < 0.002) between α and σ1. Collectively, variability in extractable metals and grain sorting accounted for ∼83% of the variability in α (at p < 0.0002) along the depth profiles. Amendments of 2.2 mg L–1 of Russian River dissolved organic carbon (DOC) reduced α for oocysts by 4–5 fold. The highly reactive hydrophobic organic acid (HPOA) fraction was particularly effective in re-entraining sediment-attached microspheres. However, the transport-enhancing effects of the riverine DOC did not appear to penetrate very deeply into the underlying sediments, judging from high α values (∼1.0) observed for oocysts being advected through unamended sediments collected at ∼2 m bls. This study suggests that in evaluating the efficacy of RBF operations to remove oocysts, it may be necessary to consider not only the geochemical nature and size distribution of the sediment grains, but also the degrees of sediment sorting and the concentration, reactivity, and penetration of the source water DOC.

Panchromatic observations of dwarf starburst galaxies: Infant super star clusters and a low-luminosity AGN

NASA Astrophysics Data System (ADS)

Reines, Amy Ellen

2011-01-01

Globular star clusters and supermassive black holes are fundamental components of today's massive galaxies, with origins dating back to the very early universe. Both globular clusters and the seeds of supermassive black holes are believed to have formed in the progenitors of modern massive galaxies, although the details are poorly understood. Direct observations of these low-mass, distant, and hence faint systems are unobtainable with current capabilities. However, gas-rich dwarf starburst galaxies in the local universe, analogous in many ways to protogalaxies at high-redshift, can provide critical insight into the early stages of galaxy evolution including the formation of globular clusters and massive black holes. This thesis presents a panchromatic study of nearby dwarf starburst galaxies harboring nascent globular clusters still embedded in their birth material. Infant clusters are identified via their production of thermal radio emission at centimeter wavelengths, which comes from dense gas ionized by young massive stars. By combining radio observations with complementary data at ultraviolet, optical and infrared wavelengths, we obtain a comprehensive view of massive clusters emerging from their gaseous and dusty birth cocoons. This thesis also presents the first example of a nearby dwarf starburst galaxy hosting an actively accreting massive central black hole. The black hole in this dwarf galaxy is unusual in that it is not associated with a bulge, a nuclear star cluster, or any other well-defined nucleus, likely reflecting an early phase of black hole and galaxy evolution that has not been previously observed.

Effects of slope on the formation of dunes in dilute, turbulent pyroclastic currents: May 18th, 1980 Mt. St. Helens eruption

NASA Astrophysics Data System (ADS)

Bendana, Sylvana; Brand, Brittany D.; Self, Stephen

2014-05-01

The flanks of Mt St Helens volcano (MSH) are draped with thin, cross-stratified and stratified pyroclastic density current (PDC) deposits. These are known as the proximal bedded deposits produced during the May 18th, 1980 eruption of MSH. While the concentrated portions of the afternoon PDCs followed deep topographic drainages down the steep flanks of the volcano, the dilute overriding cloud partially decoupled to develop fully dilute, turbulent PDCs on the flanks of the volcano (Beeson, D.L. 1988. Proximal Flank Facies of the May 18, 1980 Ignimbrite: Mt. St. Helens, Washington.). The deposits along the flank thus vary greatly from those found in the pumice plain, which are generally thick, massive, poorly-sorted, block-rich deposits associated with the more concentrated portions of the flow (Brand et al, accepted. Dynamics of pyroclastic density currents: Conditions that promote substrate erosion and self-channelization - Mount St Helens, Washington (USA). JVGR). We explore the influence of topography on the formation of these dilute currents and influence of slope on the currents transport and depositional mechanisms. The deposits on steeper slopes (>15°) are fines depleted relative to the proximal bedded deposits on shallower slopes (<15°). Bedform amplitude and wavelength increase with increasing slope, as does the occurrence of regressive dunes. Increasing slope causes an increase in flow velocity and thus an increase in flow turbulence. The fines depleted deposits suggest that fine ash elutriation is more efficient in flows with stronger turbulence. The longer wavelength and amplitudes suggest that bedform morphology is directly related to flow velocity, an important finding since the controls on bedform wavelength and amplitude in density stratified flows remains poorly constrained. The occurrence of regressive dunes, often interpreted as high flow-regime bedforms, on steeper slopes relative to progressive dunes on shallower slopes further attests to the control of velocity and flow regime on bedform morphology. Samples collected from recently exposed deposits and analyzed by grain size measurements, density analyses, and crystal morphoscopy studies further assess modes of origin and transport of dilute PDCs.

New approach for detecting and classifying concealed weapons

NASA Astrophysics Data System (ADS)

Roybal, Lyle G.; Rice, Philip M.; Manhardt, Joseph M.

1997-02-01

The possession of a weapon in the courtroom of our American justice system is a Federal offense. A weapon in the courtroom is a particularly serious problem for judges, plaintiffs, defendants, and bystanders because of the emotional nature and environment of the courtroom. Potential violators include organized criminals and gangsters, individuals with personal vendettas, and otherwise sane and reasonable people who, because of the emotion of the moment, lose sight of right and wrong in our justice system. Most Federal courthouses incorporate some sort of metal detection apparatus used to screen and prevent individuals from carrying personal weapons into courtrooms. These devices are considered conventional in the sense that they employ an active electromagnetic induction technique that is sensitive to the presence of metal. This technique provides no information concerning the massiveness and location of the offending metal object and is prone to false alarms. This work introduces an alternative method for weapons discrimination that is based on passive magnetics technology in concert with simple comparative algorithms such that massiveness and location of suspected weapons may be determined. Since this system provides information not available from conventional metal detectors, false alarms will be greatly reduced, allowing remote monitoring of all entrances into a courtroom from a single vantage point, thereby saving considerable resources used on personnel costs.

Nacre-Templated Synthesis of Highly Dispersible Carbon Nanomeshes for Layered Membranes with High-Flux Filtration and Sensing Properties.

PubMed

Kong, Meng; Li, Mingjie; Shang, Ruoxu; Wu, Jingyu; Yan, Peisong; Xu, Dongmei; Li, Chaoxu

2018-01-24

Marine shells not only represent a rapidly accumulating type of fishery wastes but also offer a unique sort of hybrid nanomaterials produced greenly and massively in nature. The elaborate "brick and mortar" structures of nacre enabled the synthesis of carbon nanomeshes with <1 nm thickness, hierarchical porosity, and high specific surface area through pyrolysis, in which two-dimensional (2D) organic layers served as the carbonaceous precursor and aragonite platelets as the hard template. Mineral bridges within 2D organic layers templated the formation of mesh pores of 20-70 nm. In contrast to other hydrophobic carbon nanomaterials, these carbon nanomeshes showed super dispersibility in diverse solvents and thus processability for membranes through filtration, patterning, spray-coating, and ink-writing. The carbon membranes with layered structures were capable of serving not only for high-flux filtration and continuous flow absorption but also for electrochemical and strain sensing with high sensitivity. Thus, utilization of marine shells, on one hand, relieves the environmental concern of shellfish waste, on the other hand, offers a facile, green, low-cost, and massive approach to synthesize unique carbon nanomeshes alternative to graphene nanomeshes and applicable in environmental adsorption, filtration, wearable sensors, and flexible microelectronics.

Molecular evolution of Adh and LEAFY and the phylogenetic utility of their introns in Pyrus (Rosaceae)

PubMed Central

2011-01-01

Background The genus Pyrus belongs to the tribe Pyreae (the former subfamily Maloideae) of the family Rosaceae, and includes one of the most important commercial fruit crops, pear. The phylogeny of Pyrus has not been definitively reconstructed. In our previous efforts, the internal transcribed spacer region (ITS) revealed a poorly resolved phylogeny due to non-concerted evolution of nrDNA arrays. Therefore, introns of low copy nuclear genes (LCNG) are explored here for improved resolution. However, paralogs and lineage sorting are still two challenges for applying LCNGs in phylogenetic studies, and at least two independent nuclear loci should be compared. In this work the second intron of LEAFY and the alcohol dehydrogenase gene (Adh) were selected to investigate their molecular evolution and phylogenetic utility. Results DNA sequence analyses revealed a complex ortholog and paralog structure of Adh genes in Pyrus and Malus, the pears and apples. Comparisons between sequences from RT-PCR and genomic PCR indicate that some Adh homologs are putatively nonfunctional. A partial region of Adh1 was sequenced for 18 Pyrus species and three subparalogs representing Adh1-1 were identified. These led to poorly resolved phylogenies due to low sequence divergence and the inclusion of putative recombinants. For the second intron of LEAFY, multiple inparalogs were discovered for both LFY1int2 and LFY2int2. LFY1int2 is inadequate for phylogenetic analysis due to lineage sorting of two inparalogs. LFY2int2-N, however, showed a relatively high sequence divergence and led to the best-resolved phylogeny. This study documents the coexistence of outparalogs and inparalogs, and lineage sorting of these paralogs and orthologous copies. It reveals putative recombinants that can lead to incorrect phylogenetic inferences, and presents an improved phylogenetic resolution of Pyrus using LFY2int2-N. Conclusions Our study represents the first phylogenetic analyses based on LCNGs in Pyrus. Ancient and recent duplications lead to a complex structure of Adh outparalogs and inparalogs in Pyrus and Malus, resulting in neofunctionalization, nonfunctionalization and possible subfunctionalization. Among all investigated orthologs, LFY2int2-N is the best nuclear marker for phylogenetic reconstruction of Pyrus due to suitable sequence divergence and the absence of lineage sorting. PMID:21917170

Endosomal sorting complexes required for ESCRTing cells toward death during neurogenesis, neurodevelopment and neurodegeneration.

PubMed

Kaul, Zenia; Chakrabarti, Oishee

2018-03-25

The endosomal sorting complexes required for transport (ESCRT) proteins help in the recognition, sorting and degradation of ubiquitinated cargoes from the cell surface, long-lived proteins or aggregates, and aged organelles present in the cytosol. These proteins take part in the endo-lysosomal system of degradation. The ESCRT proteins also play an integral role in cytokinesis, viral budding and mRNA transport. Many neurodegenerative diseases are caused by toxic accumulation of cargo in the cell, which causes stress and ultimately leads to neuronal death. This accumulation of cargo occurs because of defects in the endo-lysosomal degradative pathway-loss of function of ESCRTs has been implicated in this mechanism. ESCRTs also take part in many survival processes, lack of which can culminate in neuronal cell death. While the role played by the ESCRT proteins in maintaining healthy neurons is known, their role in neurodegenerative diseases is still poorly understood. In this review, we highlight the importance of ESCRTs in maintaining healthy neurons and then suggest how perturbations in many of the survival mechanisms governed by these proteins could eventually lead to cell death; quite often these correlations are not so obviously laid out. Extensive neuronal death eventually culminates in neurodegeneration. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

"My Heart Die in Me": Idioms of Distress and the Development of a Screening Tool for Mental Suffering in Southeast Liberia.

PubMed

Fabian, Katrin; Fannoh, Josiah; Washington, George G; Geninyan, Wilfred B; Nyachienga, Bethuel; Cyrus, Garmai; Hallowanger, Joyce N; Beste, Jason; Rao, Deepa; Wagenaar, Bradley H

2018-05-04

The integration of culturally salient idioms of distress into mental healthcare delivery is essential for effective screening, diagnosis, and treatment. This study systematically explored idioms, explanatory models, and conceptualizations in Maryland County, Liberia to develop a culturally-resonant screening tool for mental distress. We employed a sequential mixed-methods process of: (1) free-lists and semi-structured interviews (n = 20); patient chart reviews (n = 315); (2) pile-sort exercises, (n = 31); and (3) confirmatory focus group discussions (FGDs); (n = 3) from June to December 2017. Free-lists identified 64 idioms of distress, 36 of which were eliminated because they were poorly understood, stigmatizing, irrelevant, or redundant. The remaining 28 terms were used in pile-sort exercises to visualize the interrelatedness of idioms. Confirmatory FDGs occurred before and after the pile-sort exercise to explain findings. Four categories of idioms resulted, the most substantial of which included terms related to the heart and to the brain/mind. The final screening tool took into account 11 idioms and 6 physical symptoms extracted from patient chart reviews. This study provides the framework for culturally resonant mental healthcare by cataloguing language around mental distress and designing an emic screening tool for validation in a clinical setting.

Selective, sustained, and shift in attention in patients with diagnoses of schizophrenia.

PubMed

Hagh-Shenas, H; Toobai, S; Makaremi, A

2002-12-01

Attentional deficits are a prominent aspect of cognitive dysfunction in schizophrenia. The present study was designed to investigate attention deficit in a group of patients with diagnosis of schizophrenia. According to the segmental set theory suggested by Hogarty and Flesher, three aspects of attention problems, selective, sustained, and shift in attention, were studied. The 30 patients hospitalized on three psychiatric wards at Shiraz and Isfahan and 30 normal healthy subjects matched for age, sex, and years of education were administered a computerized Continuous Performance Test, Stroop Color-word Test, and Wisconsin Card Sorting test. Analysis showed patients performed more poorly than control subjects on measured aspects of attention. The acute/chronic classification did not predict differences in attention scores between subtypes of schizophrenia, while the positive/negative classification did. Paranoid, undifferentiated, and residual groups by subtypes of schizophrenia showed similar performance on the Continuous Performance Test, but were significantly different on errors on the Wisconsin Card Sorting test and on reaction time to Stroop stimuli in the incongruent color-word condition. Patients with paranoid diagnosis performed better than other subtypes on these tasks. Present results suggest that the Continuous Performance Test is valuable for differentiating of schizophrenia spectrum disorder, while scores on Stroop and Wisconsin card sorting may have better diagnostic value for differentiating subtypes of the disorder.

Volcanic Debris Flows of the Latest Paleozoic Arbasay Formation: Geomorphological Characters and Paleoenvironment Reconstruction of Northern Tian Shan, NW China

NASA Astrophysics Data System (ADS)

Yang, W.; Liu, D.; Guo, Z.

2015-12-01

Texturally well-preserved volcanic debris flows (also called lahars) are exposed in the Latest Paleozoic Arbasay Formation, Northern Tian Shan. LA-ICP-MS zircon dating of the intercalated fallout tuff sample provided an age of 314.4±3.4 Ma (MSWD=1.6), suggesting they were deposited at Latest Carboniferous. The lahars consist primarily of two lithofacies: massive, poorly lithified diamictites and stratified, moderately lithified gravelly sandstones. The diamictites can be generally divided into two subfacies, i.e., the matrix-supported and the clast-supported diamictites. Most diamictites are structureless and nongraded. They are thick in beds and contain large clasts up to 3 m in dimension. The gravelly sandstones display much finer particle size and have wedge or lenticular geometries. Large clasts are absent within them and the sorting characters are much better than the diamictites. Despite the different size grading, the matrix and the clasts of the two lithofacies appear to be homogeneous. The matrix is generally sandy mudstone. The clasts comprise rhyolites, dacites, andesites, andesitic basalts and basalts, same to the co-existing volcanic rocks, suggesting they originate from the cognate volcanics. The disorganized diamictites are supposed to deposit from a turbulent flood or pyroclastic surge. The gravelly sandstone lithofacies are interpreted as sand-rich flood flows or hyperconcentrated flood flows during the waning stage of a mass-flow event. The overall characteristics of the deposits suggest a mass-flow dominated alluvial fan environment. It's noteable that several syn- sedimentary normal faults occurred within these lahar deposits, indicating that the Southern Junggar Basin was in an extensional regime during the lahars' deposition. Structure is dominated by normal faulting, allowing the existence of relatively small, highly compartmentalized depocenters. This is also supported by geochemistry and detrital zircon studies.

Mixing of biogenic siliceous and terrigenous clastic sediments: South Belridge field and Beta field, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schwartz, D.E.

1990-05-01

The intermixing and interbedding of biogenically derived siliceous sediment with terrigenous clastic sediment in reservoirs of upper Miocene age provides both reservoir rock and seal and influences productivity by affecting porosity and permeability. Miocene reservoirs commonly contain either biogenic-dominated cyclic diatomite, porcelanite, or chert (classic Monterey Formation) or clastic-dominated submarine fan sequences with interbedded or intermixed siliceous members of biogenic origin. Biogenic-clastic cycles, 30-180 ft thick, at South Belridge field were formed by episodic influx of clastic sediment from distant submarine fans mixing with slowly accumulating diatomaceous ooze. The cycles consist of basal silt and pelletized massive diatomaceous mudstone, overlainmore » by burrowed, faintly bedded clayey diatomite and topped by laminated diatomite. Cycle tops have higher porosity and permeability, lower grain density, and higher oil saturation than clay and silt-rich portions of the cycles. Submarine fan sediments forming reservoirs at the Beta field are comprised of interbedded sands and silts deposited in a channelized middle fan to outer fan setting. Individual turbidites display fining-upward sequences, with oil-bearing sands capped by wet micaceous silts. Average sands are moderately to poorly sorted, fine- to medium-grained arkosic arenites. Sands contain pore-filling carbonate and porcelaneous cements. Porcelaneous cement consists of a mixture of opal-A, opal-CT, and chert with montmorillonite and minor zeolite. This cement is an authigenic material precipitated in intergranular pore space. The origin of the opal is biogenic, with recrystallization of diatom frustules (opal-A) into opal-CT lepispheres and quartz crystals. Porcelaneous cement comprises 4-21% of the bulk volume of the rock. Seventy percent of the bulk volume of the cement is micropore space.« less

Recent Results from the Mars Exploration Rover Spirit Mission

NASA Astrophysics Data System (ADS)

Squyres, S. W.

2005-05-01

Since arriving at the Columbia Hills, the Spirit rover's primary area of geologic investigation has been the West Spur of Husband Hill. Pancam images of West Spur rocks show morphology ranging from massive to finely layered. Microscopic Imager images show the rocks to be clastic in nature, with a substantial range in grain sizes. Grains vary from rounded to angular. Mini-TES data show little variability from one rock to the next, and the best fit to the IR spectral signature of the rocks is dominated by basaltic glass. The chemistry revealed by the APXS is broadly basaltic in nature, but substantially enhanced in P, S, Cl, and Br relative to plains rocks. Moessbauer data show that olivine is absent in West Spur rocks, and pyroxene signatures are weak. Fe oxides and oxyhydroxides are present. We interpret the rocks of the West Spur to be aqueously altered basaltic materials of volcaniclastic or impact origin. Since leaving the West Spur, Spirit has explored toward the northeast, higher onto Husband Hill. Loose rocks ("float") on the north flank of the hill are dominated by another poorly sorted clastic lithology that contains olivine and that has strikingly high abundances of Ti and P. Only a few bedrock outcrops have been identified on the main body of Husband Hill. All of these examined to date consist of a coarse-grained clastic rock dominated by basaltic chemistry and cemented by sulfate salts. Grain sizes range up to several mm, and sub-cm layering is present. Moessbauer data show pyroxene, olivine, and a high abundance of magnetite in the basaltic component. APXS data are consistent with the rock being up to 20 percent magnesium sulfate salts by mass, and microscopic images show a high degree of cementation by these salts.

Translating Microfluidics: Cell Separation Technologies and their Barriers to Commercialization

PubMed Central

Shields, C. Wyatt; Ohiri, Korine A.; Szott, Lizzy M.; López, Gabriel P.

2016-01-01

Advances in microfluidic cell sorting have revolutionized the ways in which cell-containing fluids are processed, now providing performances comparable to, or exceeding, traditional systems, but in a vastly miniaturized format. These technologies exploit a wide variety of physical phenomena to manipulate cells and fluid flow, such as magnetic traps, sound waves and flow-altering micropatterns, and they can evaluate single cells by immobilizing them onto surfaces for chemotherapeutic assessment, encapsulate cells into picoliter droplets for toxicity screenings and examine the interactions between pairs of cells in response to new, experimental drugs. However, despite the massive surge of innovation in these high-performance lab-on-a-chip devices, few have undergone successful commercialization, and no device has been translated to a widely distributed clinical commodity to date. Persistent challenges such as an increasingly saturated patent landscape as well as complex user interfaces are among several factors that may contribute to their slowed progress. In this article, we identify several of the leading microfluidic technologies for sorting cells that are poised for clinical translation; we examine the principal barriers preventing their routine clinical use; finally, we provide a prospectus to elucidate the key criteria that must be met to overcome those barriers. Once established, these tools may soon transform how clinical labs study various ailments and diseases by separating cells for downstream sequencing and enabling other forms of advanced cellular or sub-cellular analysis. PMID:27282966

Mapping Next Generation Learning Spaces as a Designed Quality Enhancement Process

ERIC Educational Resources Information Center

Leonard, Simon N.; Fitzgerald, Robert N.; Bacon, Matt; Munnerley, Danny

2017-01-01

The learning spaces of higher education are changing with collaborative, agile and technology-enabled spaces ever more popular. Despite the massive investment required to create these new spaces, current quality systems are poorly placed to account for the value they create. Such learning spaces are typically popular with students but the impact…

A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructor

USDA-ARS?s Scientific Manuscript database

The mechanisms arthropods use to induce plant gall formation are poorly understood. However, there is growing evidence that effector proteins are involved. To examine this hypothesis, we sequenced the genome of the Hessian fly (Mayetiola destructor, M. des), an obligate plant parasitic gall midge an...

State Investment in Universities: Rethinking the Impact on Economic Growth

ERIC Educational Resources Information Center

Schalin, Jay

2010-01-01

Does investing taxpayer money in higher education lead to major payoffs in economic growth? State legislators and policy makers say yes. They routinely advocate massive appropriations for university education and research, even in poor economic times, on the grounds that taxpayers will be rewarded many times over. The investment of federal funds…

Tephra from the 1979 soufriere explosive eruption.

PubMed

Sigurdsson, H

1982-06-04

The explosive phase of the 1979 Soufriere eruption produced 37.5 x 10(6) cubic meters (dense-rock equivalent) of tephra, consisting of about 40 percent juvenile basaltic andesite and 60 percent of a nonjuvenile component derived from the fragmentation of the 1971-1972 lava island during phreatomagmatic explosions. The unusually fine grain size, poor sorting, and bimodality of the land deposit are attributed to particle aggregation and the formation of accretionary lapilli in a wet eruption column.

Rocks of the Columbia Hills

USGS Publications Warehouse

Squyres, S. W.; Arvidson, R. E.; Blaney, D.L.; Clark, B. C.; Crumpler, L.; Farrand, W. H.; Gorevan, S.; Herkenhoff, K. E.; Hurowitz, J.; Kusack, A.; McSween, H.Y.; Ming, D. W.; Morris, R.V.; Ruff, S.W.; Wang, A.; Yen, A.

2006-01-01

The Mars Exploration Rover Spirit has identified five distinct rock types in the Columbia Hills of Gusev crater. Clovis Class rock is a poorly sorted clastic rock that has undergone substantial aqueous alteration. We interpret it to be aqueously altered ejecta deposits formed by impacts into basaltic materials. Wishstone Class rock is also a poorly sorted clastic rock that has a distinctive chemical composition that is high in Ti and P and low in Cr. Wishstone Class rock may be pyroclastic or impact in origin. Peace Class rock is a sedimentary material composed of ultramafic sand grains cemented by significant quantities of Mg- and Ca-sulfates. Peace Class rock may have formed when water briefly saturated the ultramafic sands and evaporated to allow precipitation of the sulfates. Watchtower Class rocks are similar chemically to Wishstone Class rocks and have undergone widely varying degrees of near-isochemical aqueous alteration. They may also be ejecta deposits, formed by impacts into Wishstone-rich materials and altered by small amounts of water. Backstay Class rocks are basalt/trachybasalt lavas that were emplaced in the Columbia Hills after the other rock classes were, either as impact ejecta or by localized volcanic activity. The geologic record preserved in the rocks of the Columbia Hills reveals a period very early in Martian history in which volcanic materials were widespread, impact was a dominant process, and water was commonly present. Copyright 2006 by the American Geophysical Union.

The Rocks of the Columbia Hills

NASA Technical Reports Server (NTRS)

Squyres, Steven W.; Arvidson, Raymond E.; Blaney, Diana L.; Clark, Benton C.; Crumpler, Larry; Farrand, William H.; Gorevan, Stephen; Herkenhoff, Kenneth; Hurowitz, Joel; Kusack, Alastair;

Stellar Yields of Rotating First Stars. II. Pair-instability Supernovae and Comparison with Observations

NASA Astrophysics Data System (ADS)

Takahashi, Koh; Yoshida, Takashi; Umeda, Hideyuki

2018-04-01

Recent theory predicts that first stars are born with a massive initial mass of ≳100 M ⊙. Pair-instability supernova (PISN) is a common fate for such massive stars. Our final goal is to prove the existence of PISNe and thus the high-mass nature of the initial mass function in the early universe by conducting abundance profiling, in which properties of a hypothetical first star is constrained by metal-poor star abundances. In order to determine reliable and useful abundances, we investigate the PISN nucleosynthesis taking both rotating and nonrotating progenitors for the first time. We show that the initial and CO core mass ranges for PISNe depend on the envelope structures: nonmagnetic rotating models developing inflated envelopes have a lower shifted CO mass range of ∼70–125 M ⊙, while nonrotating and magnetic rotating models with deflated envelopes have a range of ∼80–135 M ⊙. However, we find no significant difference in explosive yields from rotating and nonrotating progenitors, except for large nitrogen production in nonmagnetic rotating models. Furthermore, we conduct the first systematic comparison between theoretical yields and a large sample of metal-poor star abundances. We find that the predicted low [Na/Mg] ∼ ‑1.5 and high [Ca/Mg] ∼0.5–1.3 abundance ratios are the most important to discriminate PISN signatures from normal metal-poor star abundances, and confirm that no currently observed metal-poor star matches with the PISN abundance. An extensive discussion on the nondetection is presented.

A single population of red globular clusters around the massive compact galaxy NGC 1277

NASA Astrophysics Data System (ADS)

Beasley, Michael A.; Trujillo, Ignacio; Leaman, Ryan; Montes, Mireia

2018-03-01

Massive galaxies are thought to form in two phases: an initial collapse of gas and giant burst of central star formation, followed by the later accretion of material that builds up their stellar and dark-matter haloes. The systems of globular clusters within such galaxies are believed to form in a similar manner. The initial central burst forms metal-rich (spectrally red) clusters, whereas more metal-poor (spectrally blue) clusters are brought in by the later accretion of less-massive satellites. This formation process is thought to result in the multimodal optical colour distributions that are seen in the globular cluster systems of massive galaxies. Here we report optical observations of the massive relic-galaxy candidate NGC 1277—a nearby, un-evolved example of a high-redshift ‘red nugget’ galaxy. We find that the optical colour distribution of the cluster system of NGC 1277 is unimodal and entirely red. This finding is in strong contrast to other galaxies of similar and larger stellar mass, the cluster systems of which always exhibit (and are generally dominated by) blue clusters. We argue that the colour distribution of the cluster system of NGC 1277 indicates that the galaxy has undergone little (if any) mass accretion after its initial collapse, and use simulations of possible merger histories to show that the stellar mass due to accretion is probably at most ten per cent of the total stellar mass of the galaxy. These results confirm that NGC 1277 is a genuine relic galaxy and demonstrate that blue clusters constitute an accreted population in present-day massive galaxies.

A single population of red globular clusters around the massive compact galaxy NGC 1277.

PubMed

Beasley, Michael A; Trujillo, Ignacio; Leaman, Ryan; Montes, Mireia

2018-03-22

Massive galaxies are thought to form in two phases: an initial collapse of gas and giant burst of central star formation, followed by the later accretion of material that builds up their stellar and dark-matter haloes. The systems of globular clusters within such galaxies are believed to form in a similar manner. The initial central burst forms metal-rich (spectrally red) clusters, whereas more metal-poor (spectrally blue) clusters are brought in by the later accretion of less-massive satellites. This formation process is thought to result in the multimodal optical colour distributions that are seen in the globular cluster systems of massive galaxies. Here we report optical observations of the massive relic-galaxy candidate NGC 1277-a nearby, un-evolved example of a high-redshift 'red nugget' galaxy. We find that the optical colour distribution of the cluster system of NGC 1277 is unimodal and entirely red. This finding is in strong contrast to other galaxies of similar and larger stellar mass, the cluster systems of which always exhibit (and are generally dominated by) blue clusters. We argue that the colour distribution of the cluster system of NGC 1277 indicates that the galaxy has undergone little (if any) mass accretion after its initial collapse, and use simulations of possible merger histories to show that the stellar mass due to accretion is probably at most ten per cent of the total stellar mass of the galaxy. These results confirm that NGC 1277 is a genuine relic galaxy and demonstrate that blue clusters constitute an accreted population in present-day massive galaxies.

OB stars at the lowest Local Group metallicity. GTC-OSIRIS observations of Sextans A

NASA Astrophysics Data System (ADS)

Camacho, I.; Garcia, M.; Herrero, A.; Simón-Díaz, S.

2016-01-01

Context. Massive stars play an important role in the chemical and dynamical evolution of the Universe. The first metal-poor stars may have started the reionization of the Universe. To understand these early epochs it is necessary to know the behavior and the physical properties of massive stars in very metal-poor environments. We focus on the massive stellar content of the metal-poor irregular galaxy Sextans A. Aims: Our aim is to find and classify OB stars in Sextans A, so as to later determine accurate stellar parameters of these blue massive stars in this low-metallicity region (Z ~ 0.1 Z⊙). Methods: Using UBV photometry, the reddening-free index Q and GALEX imaging, we built a list of blue massive star candidates in Sextans A. We obtained low-resolution (R ~ 1000) GTC-OSIRIS spectra for a fraction of them and carried out spectral classification. For the confirmed O-stars, we derived preliminary stellar parameters. Results: The target selection criteria and observations were successful and have produced the first spectroscopic atlas of OB-type stars in Sextans A. From the whole sample of 18 observed stars, 12 were classified as early OB-types, including 5 O-stars. The radial velocities of all target stars are in agreement with their Sextans A membership, although three of them show significant deviations. We determined the stellar parameters of the O-type stars using the stellar atmosphere code FASTWIND and revisited the sub-SMC temperature scale. Two of the O-stars are consistent with relatively strong winds and enhanced helium abundances, although results are not conclusive. We discuss the position of the OB stars in the HRD. Initial stellar masses run from slightly below 20 up to 40 solar masses. Conclusions: The target selection method worked well for Sextans A. The stellar temperatures are consistent with findings in other galaxies. Some of the targets deserve follow-up spectroscopy because of indications of a runaway nature, an enhanced helium abundance, or a relatively strong wind. We observe a correlation between HI and OB associations similar to the irregular galaxy IC 1613, confirming the previous result that the most recent star formation of Sextans A is currently ongoing near the rim of the H I cavity. Based on observations made with the Gran Telescopio Canarias (GTC), installed in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias, on the island of La Palma. Programme ID GTC59-12A.The data are available through the GTC archive: http://https://gtc.sdc.cab.inta-csic.es/gtc/jsp/searchres.jsp

Stratification in the lunar regolith - A preliminary view

NASA Technical Reports Server (NTRS)

Duke, M. B.; Nagle, J. S.

1975-01-01

Although our knowledge of lunar regolith stratification is incomplete, several categories of thick and thin strata have been identified. Relatively thick units average 2 to 3 cm in thickness, and appear surficially to be massive. On more detailed examination, these units can be uniformly fine-grained, can show internal trends, or can show internal variations which apparently are random. Other thick units contain soil clasts apparently reworked from underlying units. Thin laminae average approximately 1 mm in thickness; lenticular distribution and composition of some thin laminae indicates that they are fillets shed from adjacent rock fragments. Other dark fine-grained well-sorted thin laminae appear to be surficial zones reworked by micrometeorites. Interpretations of stratigraphic succession can be strengthened by the occurrence of characteristic coarse rock fragments and the orientation of large spatter agglutinates, which are commonly found in their original depositional orientation.

Massive Gastric Juvenile Polyposis: A Clinicopathologic Study Using SMAD4 Immunohistochemistry.

PubMed

Lawless, Margaret E; Toweill, Daniel L; Jewell, Kim D; Jain, Dhanpat; Lamps, Laura; Krasinskas, Alyssa M; Swanson, Paul E; Upton, Melissa P; Yeh, Matthew M

2017-04-01

Juvenile polyps involving the stomach are uncommon. Massive gastric juvenile polyposis is even rarer. We describe the clinicopathologic features of nine cases of massive gastric juvenile polyposis. All patients had anemia; four had hypoalbuminemia. The polyps were composed predominantly of dilated crypts lined by columnar epithelium and abundant edematous stroma with mixed inflammatory infiltrates. One patient had a poorly differentiated adenocarcinoma, arising in juvenile polyp-associated intraepithelial neoplasia. A second patient had a well-differentiated intramucosal adenocarcinoma arising in a juvenile polyp with high-grade dysplasia. Three of our cases had polyposis restricted to the stomach. Six (66.6%) had loss of SMAD4 immunoreactivity, making them subject to severe bleeding and hypoproteinemia, as well as developing severe dysplasia or adenocarcinoma. SMAD4 immunohistochemstry is a helpful ancillary diagnostic test in cases of suspected juvenile polyposis syndrome involving the stomach. © American Society for Clinical Pathology, 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

What shapes stellar metallicity gradients of massive galaxies at large radii?

NASA Astrophysics Data System (ADS)

Hirschmann, Michaela

2017-03-01

We investigate the differential impact of physical mechanisms, mergers and internal energetic phenomena, on the evolution of stellar metallicity gradients in massive, present-day galaxies employing sets of high-resolution, cosmological zoom simulations. We demonstrate that negative metallicity gradients at large radii (>2Reff) originate from the accretion of metal-poor stellar systems. At larger radii, galaxies become typically more dominated by stars accreted from satellite galaxies in major and minor mergers. However, only strong galactic, stellar-driven winds can sufficiently reduce the metallicity content of the accreted stars to realistically steepen the outer metallicity gradients in agreement with observations. In contrast, the gradients of the models without winds are inconsistent with observations. Moreover, we discuss the impact of additional AGN feedback. This analysis greatly highlights the importance of both energetic processes and merger events for stellar population properties of massive galaxies at large radii. Our results are expected to significantly contribute to the interpretation of current and up-coming IFU surveys (e.g. MaNGA, CALIFA).

Non-thermal Processes in Colliding-wind Massive Binaries: the Contribution of Simbol-X to a Multiwavelength Investigation

NASA Astrophysics Data System (ADS)

De Becker, Michaël; Blomme, Ronny; Micela, Giusi; Pittard, Julian M.; Rauw, Gregor; Romero, Gustavo E.; Sana, Hugues; Stevens, Ian R.

2009-05-01

Several colliding-wind massive binaries are known to be non-thermal emitters in the radio domain. This constitutes strong evidence for the fact that an efficient particle acceleration process is at work in these objects. The acceleration mechanism is most probably the Diffusive Shock Acceleration (DSA) process in the presence of strong hydrodynamic shocks due to the colliding-winds. In order to investigate the physics of this particle acceleration, we initiated a multiwavelength campaign covering a large part of the electromagnetic spectrum. In this context, the detailed study of the hard X-ray emission from these sources in the SIMBOL-X bandpass constitutes a crucial element in order to probe this still poorly known topic of astrophysics. It should be noted that colliding-wind massive binaries should be considered as very valuable targets for the investigation of particle acceleration in a similar way as supernova remnants, but in a different region of the parameter space.

[Surgical outcome of external decompression associated with anterior and medial temporal lobectomy for massive hemispheric infarction due to internal carotid artery occlusion].

PubMed

Yamazaki, Takaaki; Kamiyama, Kenji; Osato, Toshiaki; Sasaki, Takehiko; Nakagawara, Jyoji; Nakamura, Hirohiko

2010-01-01

Acute occlusion of the internal carotid artery (ICA) can lead the massive cerebral hemispheric infarction and cause massive cerebral edema and may result in tentorial herniation and death. The mortality rate is estimated at 80% with maximum conservative medical treatment. We have performed external decompression associated with anterior and medial temporal lobectomy (AMTL) as internal decompression for lifesaving. This study evaluated our surgical results and gives an analysis of the prognostic factors. Twenty one consecutive patients with massive cerebral infarction caused by internal carotid artery occlusion who underwent external decompression associated with AMTL for lifesaving between June 2000 and December 2005 were included in this retrospective analysis. Survivors were divided into two functional groups at three months after surgery: good (Barthel index; BI> or =50) and poor (B1<50). The characteristics of the two groups were compared using statistical analysis. The patients consisted of 11 males and 10 females aged from 28 to 81 years with a mean age of 65.0+/-11.6 years. Eight patients had an infarction restricted to the middle cerebral artery (MCA) territory, others had additional anterior cerebral artery (ACA) or posterior cerebral artery (PCA) territory infarctions. The mean time between stroke onset and operation was 43.5+/-30 hours and ranged from 7 to 148 hours. Two patients died, so the mortality was 9.5%. Elderly patients (> or =60 years) (P=0.038), high preoperative Japan coma scale (> or =3 digit) (P=0.013), low preoperative Glasgow coma scale (GCS<8) (P=0.044), and multiple arterial territory (MCA+ACA or PCA) infarction (P=0.045) were significantly associated with poor functional outcome. External decompression associated with AMTL can immediately relieve peduncle compression and could be effective in preserving life as effectively as "early" external decompression.

The Serotonin Transporter Undergoes Constitutive Internalization and Is Primarily Sorted to Late Endosomes and Lysosomal Degradation*

PubMed Central

Rahbek-Clemmensen, Troels; Bay, Tina; Eriksen, Jacob; Gether, Ulrik; Jørgensen, Trine Nygaard

2014-01-01

The serotonin transporter (SERT) plays a critical role in regulating serotonin signaling by mediating reuptake of serotonin from the extracellular space. The molecular and cellular mechanisms controlling SERT levels in the membrane remain poorly understood. To study trafficking of the surface resident SERT, two functional epitope-tagged variants were generated. Fusion of a FLAG-tagged one-transmembrane segment protein Tac to the SERT N terminus generated a transporter with an extracellular epitope suited for trafficking studies (TacSERT). Likewise, a construct with an extracellular antibody epitope was generated by introducing an HA (hemagglutinin) tag in the extracellular loop 2 of SERT (HA-SERT). By using TacSERT and HA-SERT in antibody-based internalization assays, we show that SERT undergoes constitutive internalization in a dynamin-dependent manner. Confocal images of constitutively internalized SERT demonstrated that SERT primarily co-localized with the late endosomal/lysosomal marker Rab7, whereas little co-localization was observed with the Rab11, a marker of the “long loop” recycling pathway. This sorting pattern was distinct from that of a prototypical recycling membrane protein, the β2-adrenergic receptor. Furthermore, internalized SERT co-localized with the lysosomal marker LysoTracker and not with transferrin. The sorting pattern was further confirmed by visualizing internalization of SERT using the fluorescent cocaine analog JHC1-64 and by reversible and pulse-chase biotinylation assays showing evidence for lysosomal degradation of the internalized transporter. Finally, we found that SERT internalized in response to stimulation with 12-myristate 13-acetate co-localized primarily with Rab7- and LysoTracker-positive compartments. We conclude that SERT is constitutively internalized and that the internalized transporter is sorted mainly to degradation. PMID:24973209

Flow Fields of the 3.5 Ga Komati Formation, South Africa: Geochemical, Stratigraphic, and Temporal relationships between Massive, Vesicular, and Spinifex flows

NASA Astrophysics Data System (ADS)

Dann, J. C.

2007-12-01

A challenge of Archean volcanology is to reconstruct submarine flow fields by mapping and analyzing vertically dipping sequences of lavas. Some flow fields are bound by sediments and/or seafloor alteration that mark clear gaps in volcanism. Flow fields in the Lower Komati Fm are defined by alternating layers of komatiite (26% MgO) and komatiitic basalt (15% MgO). Five komatiite flow fields (100-200m thick) repeat the same stratigraphic zoning of spinifex overlying massive komatiite, and each flow field has a distinct Al2O3/CaO, a ratio unaffected by olivine fractionation, consistent with the contention that each komatiite flow field represents a distinct batch of mantle melting. Although massive and spinifex komatiite form distinct stratigraphic units on a map scale, detailed outcrop mapping reveals that the change in flow type represents a transition within a single flow field. In one type of transition, thin massive flows alternate with spinifex flow lobes of a compound flow unit. In another, a vesicular flow along the boundary links the underlying massive komatiite and overlying spinifex flows in time. The vesicular flow has alternating spinifex and vesicular layers that form a distinctive crust above a thick massive interior. Locally, this crust is tilted, intruded by massive komatiite from the interior, and overlain by a thick breccia including a spinifex flow broken into blocks and rotated like dominoes by the tilting. These outcrop relations indicate that spinifex flow lobes were starting to flow over the vesicular flow before it had undergone differential inflation, a temporal link between the lower massive and upper spinifex komatiites consistent with their belonging to the same flow field. The transition in flow type may reflect 1) an overlap of proximal and distal facies of komatiite flows as eruption rates waned and/or 2) thermal maturation prior to eruption. Early, cooler, crystal-rich, massive lava, flowing out as thick sheet flows, was replaced by hotter, crystal-poor, less degassed lava, flowing out as spinifex flows.

Reminder about potentially serious problems with a type of blocked ANOVA analysis

Treesearch

Steve Verrill; David E. Kretschmann

2017-01-01

A type of blocked experiment has the potential of being poorly designed and/or analyzed. Verrill (1993, 1999) and Verrill et al. (2004) referred to such an experiment as a “predictor sort” experiment. David and Gunnink (1997) described the procedure as “artificial pairing.” In textbooks it is sometimes referred to as a “matched pair” or a “matched...

Toward Equity and Diversity in Literacy Research, Policy, and Practice: A Critical, Global Approach

ERIC Educational Resources Information Center

Morrell, Ernest

2017-01-01

Can growing inequities between rich and poor and massive manifestations of hatred and intolerance amid rising tides of global populism inspire a focus on equity and diversity in literacy research, policy, and practice? Can such calls for change be collaborative rather than competitive? Can we envision self-love, wellness, and intercultural…

Fractional crystallization-induced variations in sulfides from the Noril’sk-Talnakh mining district (polar Siberia, Russia)

USGS Publications Warehouse

Duran, C.J.; Barnes, S-J.; Pleše, P.; Prašek, M. Kudrna; Zientek, Michael L.; Pagé, P.

2017-01-01

The distribution of platinum-group elements (PGE) within zoned magmatic ore bodies has been extensively studied and appears to be controlled by the partitioning behavior of the PGE during fractional crystallization of magmatic sulfide liquids. However, other chalcophile elements, especially TABS (Te, As, Bi, Sb, and Sn) have been neglected despite their critical role in forming platinum-group minerals (PGM). TABS are volatile trace elements that are considered to be mobile so investigating their primary distribution may be challenging in magmatic ore bodies that have been somewhat altered. Magmatic sulfide ore bodies from the Noril’sk-Talnakh mining district (polar Siberia, Russia) offer an exceptional opportunity to investigate the behavior of TABS during fractional crystallization of sulfide liquids and PGM formation as the primary features of the ore bodies have been relatively well preserved. In this study, new petrographic (2D and 3D) and whole-rock geochemical data from Cu-poor to Cu-rich sulfide ores of the Noril’sk-Talnakh mining district are integrated with published data to consider the role of fractional crystallization in generating mineralogical and geochemical variations across the different ore types (disseminated to massive). Despite textural variations in Cu-rich massive sulfides (lenses, veins, and breccias), these sulfides have similar chemical compositions, which suggests that Cu-rich veins and breccias formed from fractionated sulfide liquids that were injected into the surrounding rocks. Numerical modeling using the median disseminated sulfide composition as the initial sulfide liquid composition and recent DMSS/liq and DISS/liq predicts the compositional variations observed in the massive sulfides, especially in terms of Pt, Pd, and TABS. Therefore, distribution of these elements in the massive sulfides was likely controlled by their partitioning behavior during sulfide liquid fractional crystallization, prior to PGM formation. Our observations indicate that in the Cu-poor massive sulfides the PGM formed as the result of exsolution from sulfide minerals whereas in the Cu-rich massive sulfides the PGM formed by crystallization from late-stage fractionated sulfide liquids. We suggest that the significant amount of Sn-bearing PGM may be related to crustal contamination from granodiorite, whereas As, Bi, Te, and Sb were likely added to the magma along with S from sedimentary rocks. Large PGM that are scarce and randomly distributed may account for most of the whole-rock Pt budget. Based on our results, we propose a holistic genetic model for the formation of the magmatic sulfide ore bodies of the Noril’sk-Talnakh mining district.

Compromised fidelity of endocytic synaptic vesicle protein sorting in the absence of stonin 2

PubMed Central

Kononenko, Natalia L.; Diril, M. Kasim; Puchkov, Dmytro; Kintscher, Michael; Koo, Seong Joo; Pfuhl, Gerit; Winter, York; Wienisch, Martin; Klingauf, Jürgen; Breustedt, Jörg; Schmitz, Dietmar; Maritzen, Tanja; Haucke, Volker

2013-01-01

Neurotransmission depends on the exocytic fusion of synaptic vesicles (SVs) and their subsequent reformation either by clathrin-mediated endocytosis or budding from bulk endosomes. How synapses are able to rapidly recycle SVs to maintain SV pool size, yet preserve their compositional identity, is poorly understood. We demonstrate that deletion of the endocytic adaptor stonin 2 (Stn2) in mice compromises the fidelity of SV protein sorting, whereas the apparent speed of SV retrieval is increased. Loss of Stn2 leads to selective missorting of synaptotagmin 1 to the neuronal surface, an elevated SV pool size, and accelerated SV protein endocytosis. The latter phenotype is mimicked by overexpression of endocytosis-defective variants of synaptotagmin 1. Increased speed of SV protein retrieval in the absence of Stn2 correlates with an up-regulation of SV reformation from bulk endosomes. Our results are consistent with a model whereby Stn2 is required to preserve SV protein composition but is dispensable for maintaining the speed of SV recycling. PMID:23345427

Emerging Roles for the Lysosome in Lipid Metabolism.

PubMed

Thelen, Ashley M; Zoncu, Roberto

2017-11-01

Precise regulation of lipid biosynthesis, transport, and storage is key to the homeostasis of cells and organisms. Cells rely on a sophisticated but poorly understood network of vesicular and nonvesicular transport mechanisms to ensure efficient delivery of lipids to target organelles. The lysosome stands at the crossroads of this network due to its ability to process and sort exogenous and endogenous lipids. The lipid-sorting function of the lysosome is intimately connected to its recently discovered role as a metabolic command-and-control center, which relays multiple nutrient cues to the master growth regulator, mechanistic target of rapamycin complex (mTORC)1 kinase. In turn, mTORC1 potently drives anabolic processes, including de novo lipid synthesis, while inhibiting lipid catabolism. Here, we describe the dual role of the lysosome in lipid transport and biogenesis, and we discuss how integration of these two processes may play important roles both in normal physiology and in disease. Copyright © 2017 Elsevier Ltd. All rights reserved.

COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal

PubMed Central

Xu, Peng; Hankins, Hannah M; MacDonald, Chris; Erlinger, Samuel J; Frazier, Meredith N; Diab, Nicholas S; Piper, Robert C; Jackson, Lauren P; MacGurn, Jason A

2017-01-01

The COPI coat forms transport vesicles from the Golgi complex and plays a poorly defined role in endocytic trafficking. Here we show that COPI binds K63-linked polyubiquitin and this interaction is crucial for trafficking of a ubiquitinated yeast SNARE (Snc1). Snc1 is a v-SNARE that drives fusion of exocytic vesicles with the plasma membrane, and then recycles through the endocytic pathway to the Golgi for reuse in exocytosis. Removal of ubiquitin from Snc1, or deletion of a β'-COP subunit propeller domain that binds K63-linked polyubiquitin, disrupts Snc1 recycling causing aberrant accumulation in internal compartments. Moreover, replacement of the β'-COP propeller domain with unrelated ubiquitin-binding domains restores Snc1 recycling. These results indicate that ubiquitination, a modification well known to target membrane proteins to the lysosome or vacuole for degradation, can also function as recycling signal to sort a SNARE into COPI vesicles in a non-degradative pathway. PMID:29058666

Sedimentary deposits of the 26 December 2004 tsunami on the northwest coast of Aceh, Indonesia

USGS Publications Warehouse

Moore, A.; Nishimura, Y.; Gelfenbaum, G.; Kamataki, T.; Triyono, R.

2006-01-01

The 2004 Sumatra-Andaman tsunami flooded coastal northern Sumatra to a depth of over 20 m, deposited a discontinuous sheet of sand up to 80 cm thick, and left mud up to 5 km inland. In most places the sand sheet is normally graded, and in some it contains complex internal stratigraphy. Structures within the sand sheet may record the passage of up to 3 individual waves. We studied the 2004 tsunami deposits in detail along a flow-parallel transect about 400 m long, 16 km southwest of Banda Aceh. Near the shore along this transect, the deposit is thin or absent. Between 50 and 400 m inland it ranges in thickness from 5 to 20 cm. The main trend in thickness is a tendency to thicken by filling low spots, most dramatically at pre-existing stream channels. Deposition generally attended inundation - along the transect, the tsunami deposited sand to within about 40 m of the inundation limit. Although the tsunami deposit contains primarily material indistinguishable from material found on the beach one month after the event, it also contains grain sizes and compositions unavailable on the current beach. Along the transect we studied, these grains become increasingly dominant both landward and upward in the deposit; possibly some landward source of sediment was exposed and exploited by the passage of the waves. The deposit also contains the unabraded shells of subtidal marine organisms, suggesting that at least part of the deposit came from offshore. Grain sizes within the deposit tend to fine upward and landward, although individual units within the deposit appear massive, or show reverse grading. Sorting becomes better landward, although the most landward sites generally become poorly sorted from the inclusion of soil clasts. These sites commonly show interlayering of sandy units and soil clast units. Deposits from the 2004 tsunami in Sumatra demonstrate the complex nature of the deposits of large tsunamis. Unlike the deposits of smaller tsunamis, internal stratigraphy is complex, and will require some effort to understand. The Sumatra deposits also show the contribution of multiple sediment sources, each of which has its own composition and grain size. Such complexity may allow more accurate modeling of flow depth and flow velocity for paleotsunamis, if an understanding of how tsunami hydraulics affect sedimentation can be established. Copyright ?? The Society of Geomagnetism and Earth, Planetary and Space Sciences (SGEPSS); The Seismological Society of Japan; The Volcanological Society of Japan; The Geodetic Society of Japan; The Japanese Society for Planetary Sciences; TERRAPUB.

Rab9-dependent retrograde transport and endosomal sorting of the endopeptidase furin

PubMed Central

Chia, Pei Zhi Cheryl; Gasnereau, Isabelle; Lieu, Zi Zhao; Gleeson, Paul A.

2011-01-01

The endopeptidase furin and the trans-Golgi network protein TGN38 are membrane proteins that recycle between the TGN and plasma membrane. TGN38 is transported by a retromer-dependent pathway from early endosomes to the TGN, whereas the intracellular transport of furin is poorly defined. Here we have identified the itinerary and transport requirements of furin. Using internalisation assays, we show that furin transits the early and late endosomes en route to the TGN. The GTPase Rab9 and the TGN golgin GCC185, components of the late endosome-to-TGN pathway, were required for efficient TGN retrieval of furin. By contrast, TGN38 trafficking was independent of Rab9 and GCC185. To identify the sorting signals for the early endosome-to-TGN pathway, the trafficking of furin–TGN38 chimeras was investigated. The diversion of furin from the Rab9-dependent late-endosome-to-TGN pathway to the retromer-dependent early-endosome-to-TGN pathway required both the transmembrane domain and cytoplasmic tail of TGN38. We present evidence to suggest that the length of the transmembrane domain is a contributing factor in endosomal sorting. Overall, these data show that furin uses the Rab9-dependent pathway from late endosomes and that retrograde transport directly from early endosomes is dependent on both the transmembrane domain and the cytoplasmic tail. PMID:21693586

[Toxic hepatitis by consumption Herbalife products a case report].

PubMed

Chao, Sara; Anders, Margarita; Turbay, Maximiliano; Olaiz, Emiliano; Mc Cormack, Lucas; Mastai, Ricardo

2008-12-01

Toxic hepatitis by consumption Herbalife products is an affection poorly documented and with a great impact in the population due to their massive consumption. We present the case of a 63-years-old woman with probable diagnosis of toxic hepatitis secondary to the consumption of nutritional supplements Herbalife. The nutritional supplements based on natural ingredients are of massive consumption worldwide. Because they are recognized like innocuous and of non-controlled comercialization, they lack suitable controls. Although there are reported cases of hepatotoxicity and other side effects induced by these products, there is still not strong evidence to generate a positive reaction of the control organisms. We report a case of acute toxic hepatitis potencially due to the consumption of Herbalife.

A parsec-scale optical jet from a massive young star in the Large Magellanic Cloud

NASA Astrophysics Data System (ADS)

McLeod, Anna F.; Reiter, Megan; Kuiper, Rolf; Klaassen, Pamela D.; Evans, Christopher J.

2018-02-01

Highly collimated parsec-scale jets, which are generally linked to the presence of an accretion disk, are commonly observed in low-mass young stellar objects. In the past two decades, a few of these jets have been directly (or indirectly) observed from higher-mass (larger than eight solar masses) young stellar objects, adding to the growing evidence that disk-mediated accretion also occurs in high-mass stars, the formation mechanism of which is still poorly understood. Of the observed jets from massive young stars, none is in the optical regime (massive young stars are typically highly obscured by their natal material), and none is found outside of the Milky Way. Here we report observations of HH 1177, an optical ionized jet that originates from a massive young stellar object located in the Large Magellanic Cloud. The jet is highly collimated over its entire measured length of at least ten parsecs and has a bipolar geometry. The presence of a jet indicates ongoing, disk-mediated accretion and, together with the high degree of collimation, implies that this system is probably formed through a scaled-up version of the formation mechanism of low-mass stars. We conclude that the physics that govern jet launching and collimation is independent of stellar mass.

A parsec-scale optical jet from a massive young star in the Large Magellanic Cloud.

PubMed

McLeod, Anna F; Reiter, Megan; Kuiper, Rolf; Klaassen, Pamela D; Evans, Christopher J

2018-02-15

Highly collimated parsec-scale jets, which are generally linked to the presence of an accretion disk, are commonly observed in low-mass young stellar objects. In the past two decades, a few of these jets have been directly (or indirectly) observed from higher-mass (larger than eight solar masses) young stellar objects, adding to the growing evidence that disk-mediated accretion also occurs in high-mass stars, the formation mechanism of which is still poorly understood. Of the observed jets from massive young stars, none is in the optical regime (massive young stars are typically highly obscured by their natal material), and none is found outside of the Milky Way. Here we report observations of HH 1177, an optical ionized jet that originates from a massive young stellar object located in the Large Magellanic Cloud. The jet is highly collimated over its entire measured length of at least ten parsecs and has a bipolar geometry. The presence of a jet indicates ongoing, disk-mediated accretion and, together with the high degree of collimation, implies that this system is probably formed through a scaled-up version of the formation mechanism of low-mass stars. We conclude that the physics that govern jet launching and collimation is independent of stellar mass.

Performance in College Chemistry: a Statistical Comparison Using Gender and Jungian Personality Type

NASA Astrophysics Data System (ADS)

Greene, Susan V.; Wheeler, Henry R.; Riley, Wayne D.

This study sorted college introductory chemistry students by gender and Jungian personality type. It recognized differences from the general population distribution and statistically compared the students' grades with their Jungian personality types. Data from 577 female students indicated that ESFP (extroverted, sensory, feeling, perceiving) and ENFP (extroverted, intuitive, feeling, perceiving) profiles performed poorly at statistically significant levels when compared with the distribution of females enrolled in introductory chemistry. The comparable analysis using data from 422 male students indicated that the poorly performing male profiles were ISTP (introverted, sensory, thinking, perceiving) and ESTP (extroverted, sensory, thinking, perceiving). ESTJ (extroverted, sensory, thinking, judging) female students withdrew from the course at a statistically significant level. For both genders, INTJ (introverted, intuitive, thinking, judging) students were the best performers. By examining the documented characteristics of Jungian profiles that correspond with poorly performing students in chemistry, one may more effectively assist the learning process and the retention of these individuals in the fields of natural science, engineering, and technology.

Flume and field-based calibration of surrogate sensors for monitoring bedload transport

NASA Astrophysics Data System (ADS)

Mao, L.; Carrillo, R.; Escauriaza, C.; Iroume, A.

2016-01-01

Bedload transport assessment is important for geomorphological, engineering, and ecological studies of gravel-bed rivers. Bedload can be monitored at experimental stations that require expensive maintenance or by using portable traps, which allows measuring instantaneous transport rates but at a single point and at high costs and operational risks. The need for continuously measuring bedload intensity and dynamics has therefore increased the use and enhancement of surrogate methods. This paper reports on a set of flume experiments in which a Japanese acoustic pipe and an impact plate have been tested using four well-sorted and three poorly sorted sediment mixtures. Additional data were collected in a glacierized high-gradient Andean stream (Estero Morales) using a portable Bunte-type bedload sampler. Results show that the data provided by the acoustic pipe (which is amplified on 6 channels having different gains) can be calibrated for the grain size and for the intensity of transported sediments coarser than 9 mm (R2 = 0.93 and 0.88, respectively). Even if the flume-based calibration is very robust, upscaling the calibration to field applications is more challenging, and the bedload intensity could be predicted better than the grain size of transported sediments (R2 = 0.61 and 0.43, respectively). The inexpensive impact plate equipped with accelerometer could be calibrated for bedload intensity quite well in the flume but only poorly in the field (R2 = 0.16) and could not provide information on the size of transported sediments.

Leukemic optic neuropathy.

PubMed

Brown, G C; Shields, J A; Augsburger, J J; Serota, F T; Koch, P

1981-03-01

The clinical course and ophthalmic manifestations of an eight year old child with acute undifferentiated leukemia and unilateral blindness secondary to leukemic optic nerve head infiltration are described. At autopsy the involved nerve head and peripapillary retina demonstrated massive leukemic cell infiltration and hemorrhagic necrosis. This manifestation of leukemia is quite uncommon and prognosis for life in such cases is poor with existing methods of therapy.

Third Annual "Brown" Lecture in Education Research--The Flat Earth and Education: How America's Commitment to Equity Will Determine Our Future

ERIC Educational Resources Information Center

Darling-Hammond, Linda

2007-01-01

In the knowledge-based economy that characterizes the 21st century, most previously industrialized countries are making massive investments in education. The United States ranks poorly on many leading indicators, however, primarily because of the great inequality in educational inputs and outcomes between White students and non-Asian…

Mineralogy and chemistry of massive sulfide deposits from the Juan de Fuca Ridge.

USGS Publications Warehouse

Koski, R.A.; Clague, D.A.; Oudin, E.

1984-01-01

Two types of massive sulphide were dredged from one of the six vent sites located in the axial valley of the southern Juan de Fuca ridge. Type A samples are angular slabs of dark grey Zn-rich sulphide with interlayers and a thin, partly-oxidized crust of Fe-sulphide. These layered sulphide aggregates appear to be fragments of a sulphide wall enclosing an active hydrothermal vent. The outer sulphide wall is composed of colloform Fe sulphide and Fe-poor sphalerite deposited under low-T conditions when sea-water and hydrothermal fluid mix above the discharge point. Inside the wall the intensifying hydrothermal sytem deposits a higher-T assemblage of granular Fe-rich sphalerite, wurtzite, pyrite and minor Cu-Fe sulphide. Type B sulphide samples are sub-rounded, spongy-textured fragments composed almost entirely of dendritic aggregates of pale Fe-poor colloform sphalerite and opaline silica. This type of sulphide is deposited in settings peripheral to sites of focused discharge and in open spaces by moderate- to low-T fluid discharging at a slow but variable rate; the fluid becomes increasingly oxidizing, resulting in late-stage deposits of hematite, baryte and sulphur.-L.di H.

Origin of accretionary lapilli from the Pompeii and Avellino deposits of Vesuvius

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sheridan, M.F.; Wohletz, K.H.

1983-01-01

Accretionary lapilli from the Pompeii and Avellino Plinian ash deposits of Vesuvius consist of centimeter-sized spheroids composed of glass, crystal, and lithic fragments of submillimeter size. The typical structure of the lapilli consists of a central massive core surrounded by concentric layers of fine ash with concentrations of larger clasts and vesicles and a thin outer layer of dust. Clasts within the lapilli larger than 125 ..mu..m are extremely rare. The median grain-size of the fine ash is about 50 ..mu..m and the size-distribution is well sorted. Most constituent particles of accretionary lapilli display blocky shapes characteristic of grains producedmore » by phreatomagmatic hydroexplosions. We have used the scanning electron microscope (SEM) in conjunction with energy dispersive spectral analysis (EDS) to investigate the textural and chemical variation along traverses from the core to the rim of lapilli from Vesuvius.« less

A history of the American Society for Clinical Investigation

PubMed Central

Howell, Joel D.

2009-01-01

One hundred years ago, in 1909, the American Society for Clinical Investigation (ASCI) held its first annual meeting. The founding members based this new society on a revolutionary approach to research that emphasized newer physiological methods. In 1924 the ASCI started a new journal, the Journal of Clinical Investigation. The ASCI has also held an annual meeting almost every year. The society has long debated who could be a member, with discussions about whether members must be physicians, what sorts of research they could do, and the role of women within the society. The ASCI has also grappled with what else the society should do, especially whether it ought to take a stand on policy issues. ASCI history has reflected changing social, political, and economic contexts, including several wars, concerns about the ethics of biomedical research, massive increases in federal research funding, and an increasingly large and specialized medical environment. PMID:19348041

Large-scale recording of neuronal ensembles.

PubMed

Buzsáki, György

2004-05-01

How does the brain orchestrate perceptions, thoughts and actions from the spiking activity of its neurons? Early single-neuron recording research treated spike pattern variability as noise that needed to be averaged out to reveal the brain's representation of invariant input. Another view is that variability of spikes is centrally coordinated and that this brain-generated ensemble pattern in cortical structures is itself a potential source of cognition. Large-scale recordings from neuronal ensembles now offer the opportunity to test these competing theoretical frameworks. Currently, wire and micro-machined silicon electrode arrays can record from large numbers of neurons and monitor local neural circuits at work. Achieving the full potential of massively parallel neuronal recordings, however, will require further development of the neuron-electrode interface, automated and efficient spike-sorting algorithms for effective isolation and identification of single neurons, and new mathematical insights for the analysis of network properties.

[Effects of complementary food supplements on respiratory infections and diarrhea of infants and young children in poor rural].

PubMed

Yu, Dongmei; Wang, Yuying; Wang, Fuzhen

2007-05-01

To investigate the effect of complementary food supplements with protein and multi-micronutrients on respiratory infections and diarrhea of infants and yound children in poor rural China. In 5 poor county of Gansu Province, 1478 children aged 4 - 12 months were enrolled and divided into two groups. In addition to the usual home-made complementary food, all the children were fed one package of either formula I or formula II per day, usual home-made complementary food, all the children were fed one package of either formula I or formula II per day, protein and micronutrient were supplemented in formula I group. Every 6 months, a massive dose of vitamin A was protein and micronutrient were supplemented in formula I group. Every 6 months, a massive dose of vitamin A was supplemented to all children, weight and height measurements have been done every 3 months until they were 24 months old. During the follow-up of 12 months supplementation, prevalence of respiratory infection and diarrhea reduce significantly compared with baseline survey (P < 0.0001), there is no difference between the two group at the same survey, lasting time of respiratory infection is shortened in formula I group, and medical cost of disease in the two groups Complementary food supplements, with large-dose vitamin A, reduce prevalence of reduced significantly. Complementary food supplements, with large-dose vitamin A, reduce prevalence of respiratory infections, diarrhea and medical cost of those diseases.

Mobility of pyroclastic density currents

NASA Astrophysics Data System (ADS)

Giordano, G.; Porreca, M.; Lesti, C.; Cas, R. A. F.

2012-04-01

Mobility of pyroclastic density currents is a hot topic largely still poorly understood. Here we review three case studies of low aspect ratio (10-4) ignimbrites that encompass the spectrum from small to large volume, from basic to felsic in composition and from hot magmatic to cold phreatomagmatic endmembers. The 0.87 km3, phreatomagmatic, K-foiditic, Peperino Albano ignimbrite (Colli Albani, Italy), was erupted from the Albano maar at < 23 ka. The ignimbrite displays both thick valley pond and veneer facies. The juvenile component is 30-40% of the total volume and is highly fragmented to ash, with only a very minor proportion of small, vesicular lapilli. The unit reaches 10 km from vent, where it is confined in major valleys. Emplacement temperatures retrieved from paleomagnetic data and field data are at 350°-100°C. The 69 km3, tephritic, Pozzolane Rosse ignimbrite was erupted from the caldera of Colli Albani at 460 ka. The succession starts with subplinian fallout of poorly vesicular scoria lapilli. The overlying ignimbrite cover more than 2000 km2 and relate to pyroclastic flows with significant mobility, able to surmount hills at more than 20 km from vent. The facies is almost ubiquitously massive and chaotic. Juvenile pyroclasts are made of variably porphyritic, poorly to moderately vesicular scoria and spatter lapilli, and coarse ash. The texture of juvenile clasts indicates that the presence of little fine ash is not due to elutriation but to weak fragmentation of poorly vesicular and poorly viscous magma. The > 500 km3, rhyodacitic Galan ignimbrite (Altiplano Puna, Argentina) was erupted at 2.1 Ma. There is no basal fallout deposit. The ignimbrite is lithic poor, very crystal rich, massive and chaotic throughout, emplaced above Curie temperature, and develops valley confined facies, but no veneer facies, from proximal to distal (> 80 km) locations. The three cases show that: - the mobility of pyroclastic flows does not necessarily relate to the conversion of potential energy into kinetic energy during the collapse of an initially buoyant column; - extreme fragmentation and entrapment of fine ash does not seem to be a pre-requisite for mobility; - temperature also seems not to be a pre-requisite.

Helicopter Propulsion Systems

DTIC Science & Technology

1981-09-01

doessais do torborsotour MAKILA nous avons choisi trois easais gui ont pour objet die asiuier qoolguos ones des conditions oxposees prece6demisont et gui...46laLore one lin dv d~bit. Los ossais ao banc consistent A 6rablir cette loi poor 6n fonictionnement aui eol do sorte I cqutiie couvrei lensemble du...laisserons de c6t6 le facteur de ddcroissanco du prix en fonction du rang de s6rie ( loi de Wright) parce que, do par sa g6ndralit6, il n’est pas influenc6

Fermentation process using specific oxygen uptake rates as a process control

DOEpatents

Van Hoek, Pim; Aristidou, Aristos; Rush, Brian J.

2016-08-30

Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

Fermentation process using specific oxygen uptake rates as a process control

DOEpatents

Van Hoek, Pim [Minnetonka, MN; Aristidou, Aristos [Maple Grove, MN; Rush, Brian [Minneapolis, MN

2011-05-10

Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

Fermentation process using specific oxygen uptake rates as a process control

DOEpatents

Hoek, Van; Pim, Aristidou [Minnetonka, MN; Aristos, Rush [Maple Grove, MN; Brian, [Minneapolis, MN

2007-06-19

Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

Fermentation process using specific oxygen uptake rates as a process control

DOEpatents

Van Hoek, Pim; Aristidou, Aristos; Rush, Brian

2014-09-09

Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

USA: Economics, Politics, Ideology, Number 12, December 1977

DTIC Science & Technology

1978-01-19

which will guarantee the pioneer firm the neces- sary profit level. The structure of market prices, however, represents a poor reflection, as we...the timely and rapid rearrangement of structural proportions. The economic mechanism of state-monopolistic capitalism, however, was incapable of...ensuring the necessary dynamism in the large-scale economy. The development of massive structural changes in the American economy is a complex and

The Next Generation Fornax Survey (NGFS). IV. Mass and Age Bimodality of Nuclear Clusters in the Fornax Core Region

NASA Astrophysics Data System (ADS)

Ordenes-Briceño, Yasna; Puzia, Thomas H.; Eigenthaler, Paul; Taylor, Matthew A.; Muñoz, Roberto P.; Zhang, Hongxin; Alamo-Martínez, Karla; Ribbeck, Karen X.; Grebel, Eva K.; Ángel, Simón; Côté, Patrick; Ferrarese, Laura; Hilker, Michael; Lançon, Ariane; Mieske, Steffen; Miller, Bryan W.; Rong, Yu; Sánchez-Janssen, Ruben

2018-06-01

We present the analysis of 61 nucleated dwarf galaxies in the central regions (≲R vir/4) of the Fornax galaxy cluster. The galaxies and their nuclei are studied as part of the Next Generation Fornax Survey using optical imaging obtained with the Dark Energy Camera mounted at Blanco/Cerro Tololo Inter-American Observatory and near-infrared data obtained with VIRCam at VISTA/ESO. We decompose the nucleated dwarfs in nucleus and spheroid, after subtracting the surface brightness profile of the spheroid component and studying the nucleus using point source photometry. In general, nuclei are consistent with colors of confirmed metal-poor globular clusters, but with significantly smaller dispersion than other confirmed compact stellar systems in Fornax. We find a bimodal nucleus mass distribution with peaks located at {log}({{ \\mathcal M }}* /{M}ȯ )≃ 5.4 and ∼6.3. These two nucleus subpopulations have different stellar population properties: the more massive nuclei are older than ∼2 Gyr and have metal-poor stellar populations (Z ≤ 0.02 Z ⊙), while the less massive nuclei are younger than ∼2 Gyr with metallicities in the range 0.02 < Z/Z ⊙ ≤ 1. We find that the nucleus mass ({{ \\mathcal M }}nuc}) versus galaxy mass ({{ \\mathcal M }}gal}) relation becomes shallower for less massive galaxies starting around 108 M ⊙, and the mass ratio {η }n={{ \\mathcal M }}nuc}/{{ \\mathcal M }}gal} shows a clear anticorrelation with {{ \\mathcal M }}gal} for the lowest masses, reaching 10%. We test current theoretical models of nuclear cluster formation and find that they cannot fully reproduce the observed trends. A likely mixture of in situ star formation and star cluster mergers seems to be acting during nucleus growth over cosmic time.

Roche-lobe overflow systems powered by black holes in young star clusters: the importance of dynamical exchanges

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mapelli, Michela; Zampieri, Luca, E-mail: michela.mapelli@oapd.inaf.it

2014-10-10

We have run 600 N-body simulations of intermediate-mass (∼3500 M {sub ☉}) young star clusters (SCs; with three different metallicities (Z = 0.01, 0.1, and 1 Z {sub ☉}). The simulations include the dependence of stellar properties and stellar winds on metallicity. Massive stellar black holes (MSBHs) with mass >25 M {sub ☉} are allowed to form through direct collapse of very massive metal-poor stars (Z < 0.3 Z {sub ☉}). We focus on the demographics of black hole (BH) binaries that undergo mass transfer via Roche lobe overflow (RLO). We find that 44% of all binaries that undergo anmore » RLO phase (RLO binaries) formed through dynamical exchange. RLO binaries that formed via exchange (RLO-EBs) are powered by more massive BHs than RLO primordial binaries (RLO-PBs). Furthermore, the RLO-EBs tend to start the RLO phase later than the RLO-PBs. In metal-poor SCs (0.01-0.1 Z {sub ☉}), >20% of all RLO binaries are powered by MSBHs. The vast majority of RLO binaries powered by MSBHs are RLO-EBs. We have produced optical color-magnitude diagrams of the simulated RLO binaries, accounting for the emission of both the donor star and the irradiated accretion disk. We find that RLO-PBs are generally associated with bluer counterparts than RLO-EBs. We compare the simulated counterparts with the observed counterparts of nine ultraluminous X-ray sources. We discuss the possibility that IC 342 X-1, Ho IX X-1, NGC 1313 X-2, and NGC 5204 X-1 are powered by an MSBH.« less

Ejection of the Massive Hydrogen-rich Envelope Timed with the Collapse of the Stripped SN 2014C

PubMed Central

Margutti, Raffaella; Kamble, A.; Milisavljevic, D.; Zapartas, E.; de Mink, S. E.; Drout, M.; Chornock, R.; Risaliti, G.; Zauderer, B. A.; Bietenholz, M.; Cantiello, M.; Chakraborti, S.; Chomiuk, L.; Fong, W.; Grefenstette, B.; Guidorzi, C.; Kirshner, R.; Parrent, J. T.; Patnaude, D.; Soderberg, A. M.; Gehrels, N. C.; Harrison, F.

2017-01-01

We present multi-wavelength observations of SN 2014C during the first 500 days. These observations represent the first solid detection of a young extragalactic stripped-envelope SN out to high-energy X-rays ~40 keV. SN 2014C shows ordinary explosion parameters (Ek ~ 1.8 × 1051 erg and Mej ~ 1.7 M⊙). However, over an ~1 year timescale, SN 2014C evolved from an ordinary hydrogen-poor supernova into a strongly interacting, hydrogen-rich supernova, violating the traditional classification scheme of type-I versus type-II SNe. Signatures of the SN shock interaction with a dense medium are observed across the spectrum, from radio to hard X-rays, and revealed the presence of a massive shell of ~1 M⊙of hydrogen-rich material at ~6 × 1016 cm. The shell was ejected by the progenitor star in the decades to centuries before collapse. This result challenges current theories of massive star evolution, as it requires a physical mechanism responsible for the ejection of the deepest hydrogen layer of H-poor SN progenitors synchronized with the onset of stellar collapse. Theoretical investigations point at binary interactions and/or instabilities during the last nuclear burning stages as potential triggers of the highly time-dependent mass loss. We constrain these scenarios utilizing the sample of 183 SNe Ib/c with public radio observations. Our analysis identifies SN 2014C-like signatures in ~10% of SNe. This fraction is reasonably consistent with the expectation from the theory of recent envelope ejection due to binary evolution if the ejected material can survive in the close environment for 103–104 years. Alternatively, nuclear burning instabilities extending to core C-burning might play a critical role. PMID:28684881

Detailed abundances for a large sample of giant stars in the globular cluster 47 Tucanae (NGC 104)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cordero, M. J.; Pilachowski, C. A.; Johnson, C. I.

2014-01-01

47 Tuc is an ideal target to study chemical evolution and globular cluster (GC) formation in massive more metal-rich GCs, as it is the closest massive GC. We present chemical abundances for O, Na, Al, Si, Ca, Ti, Fe, Ni, La, and Eu in 164 red giant branch stars in the massive GC 47 Tuc using spectra obtained with both the Hydra multifiber spectrograph at the Blanco 4 m telescope and the FLAMES multiobject spectrograph at the Very Large Telescope. We find an average [Fe/H] = –0.79 ± 0.09 dex, consistent with literature values, as well as overabundances of alpha-elementsmore » ([α/Fe] ∼ 0.3 dex). The n-capture process elements indicate that 47 Tuc is r process-dominated ([Eu/La] = +0.24), and the light elements O, Na, and Al exhibit star-to-star variations. The Na-O anticorrelation, a signature typically seen in Galactic GCs, is present in 47 Tuc, and extends to include a small number of stars with [O/Fe] ∼ –0.5. Additionally, the [O/Na] ratios of our sample reveal that the cluster stars can be separated into three distinct populations. A Kolmogorov-Smirnov test demonstrates that the O-poor/Na-rich stars are more centrally concentrated than the O-rich/Na-poor stars. The observed number and radial distribution of 47 Tuc's stellar populations, as distinguished by their light element composition, agrees closely with the results obtained from photometric data. We do not find evidence supporting a strong Na-Al correlation in 47 Tuc, which is consistent with current models of asymptotic giant branch nucleosynthesis yields.« less

Development of a novel cell sorting method that samples population diversity in flow cytometry.

PubMed

Osborne, Geoffrey W; Andersen, Stacey B; Battye, Francis L

2015-11-01

Flow cytometry based electrostatic cell sorting is an important tool in the separation of cell populations. Existing instruments can sort single cells into multi-well collection plates, and keep track of cell of origin and sorted well location. However currently single sorted cell results reflect the population distribution and fail to capture the population diversity. Software was designed that implements a novel sorting approach, "Slice and Dice Sorting," that links a graphical representation of a multi-well plate to logic that ensures that single cells are sampled and sorted from all areas defined by the sort region/s. Therefore the diversity of the total population is captured, and the more frequently occurring or rarer cell types are all sampled. The sorting approach was tested computationally, and using functional cell based assays. Computationally we demonstrate that conventional single cell sorting can sample as little as 50% of the population diversity dependant on the population distribution, and that Slice and Dice sorting samples much more of the variety present within a cell population. We then show by sorting single cells into wells using the Slice and Dice sorting method that there are cells sorted using this method that would be either rarely sorted, or not sorted at all using conventional single cell sorting approaches. The present study demonstrates a novel single cell sorting method that samples much more of the population diversity than current methods. It has implications in clonal selection, stem cell sorting, single cell sequencing and any areas where population heterogeneity is of importance. © 2015 International Society for Advancement of Cytometry.

A task analysis of the shift from teacher instructions to self-instructions in performing an in-common task.

PubMed

Grote, I; Rosales, J; Baer, D M

1996-11-01

Three preschool children repeatedly did four kinds of sorts with a deck of stimulus cards: a difficult, untaught target sort and three other sorts considered analytic of self-instructing the target performance. The untaught target sort was to find in a deck of cards those matching what two sample cards had in common. Most preschool children must be taught to mediate this problem. The three other kinds of sorts taught skills involved in the target performance or its mediation. As correct self-instructive talk emerged in the target sorts, it was confirmed. The untaught target sorts were interspersed infrequently among the three alternating directly taught skill sorts, to see if accurate target sorts, and accurate self-instructive talk about the target sorts, would emerge as the three skill sorts were mastered. As all the sorts progressed, increasing accuracy was seen first in the skill sorts and then in the untaught target sorts. All three subjects showed subsequent generalization to new target sorts involving other stimulus sets. Correct spontaneous self-instructions about the target sorts increased from near zero at the beginning of the experiment to consistency at its end. Thus the three skill sorts appeared sufficient for the emergence of a self-instructed solution to the previously insoluble target performance.

Parallel sort with a ranged, partitioned key-value store in a high perfomance computing environment

DOEpatents

Bent, John M.; Faibish, Sorin; Grider, Gary; Torres, Aaron; Poole, Stephen W.

2016-01-26

Improved sorting techniques are provided that perform a parallel sort using a ranged, partitioned key-value store in a high performance computing (HPC) environment. A plurality of input data files comprising unsorted key-value data in a partitioned key-value store are sorted. The partitioned key-value store comprises a range server for each of a plurality of ranges. Each input data file has an associated reader thread. Each reader thread reads the unsorted key-value data in the corresponding input data file and performs a local sort of the unsorted key-value data to generate sorted key-value data. A plurality of sorted, ranged subsets of each of the sorted key-value data are generated based on the plurality of ranges. Each sorted, ranged subset corresponds to a given one of the ranges and is provided to one of the range servers corresponding to the range of the sorted, ranged subset. Each range server sorts the received sorted, ranged subsets and provides a sorted range. A plurality of the sorted ranges are concatenated to obtain a globally sorted result.

Sediment grain-size characteristics and relevant correlations to the aeolian environment in China's eastern desert region.

PubMed

Zhang, Chunlai; Shen, Yaping; Li, Qing; Jia, Wenru; Li, Jiao; Wang, Xuesong

2018-06-15

To identify characteristics of aeolian activity and the aeolian environment in China's eastern desert region, this study collected surface sediment samples from the main desert and sandy lands in this region: the Hobq Desert and the Mu Us, Otindag, Horqin, and Hulunbuir sandy lands. We analyzed the grain-size characteristics and their relationships to three key environmental indicators: drift potential, the dune mobility index, and vegetation cover. The main sediment components are fine to medium sands, with poor (Hulunbuir) to moderate (all other areas) sorting, of unimodal to bimodal distribution. This suggests that improved sorting is accomplished by the loss of both relatively coarser and finer grains. Since 2000, China's eastern desert region has generally experienced low wind energy environmental conditions, resulting in decreased dune activity. In the Hobq Desert, however, the dry climate and sparse vegetation, in conjunction with the most widely distributed mobile dune area in the eastern desert region, have led to frequent and intense aeolian activity, including wind erosion, sand transport, and deposition, resulting in conditions for good sediment sorting. In the Mu Us, Otindag, and Horqin sandy lands, mosaic distribution has resulted from wind erosion-dominated and deposition-dominated aeolian environments. In the Hulunbuir Sandy Land, high precipitation, low temperatures, and steppe vegetation have resulted in well-developed soils; however, strong winds and flat terrain have created an aeolian environment dominated by wind erosion. Copyright © 2018. Published by Elsevier B.V.

Carbonate pseudomatrix in siliciclastic-carbonate turbidites from the Oquirrh-Wood River basin, southern Idaho

DOE Office of Scientific and Technical Information (OSTI.GOV)

Geslin, J.K.

1994-01-01

Upper Pennsylvanian to Lower Permian mixed siliciclastic-carbonate sandy turbidites from the Oquirrh-Wood River basin in southern Idaho contain 20 to 60 modal percent microspar and pseudospar. Previous interpretations suggested that neomorphism of detrital lime mud produced the observed carbonate textures. The original detrital lime mud produced the observed carbonate textures. The original detrital lime mud content, based on these interpretations, indicates matrix-rich, poorly sorted turbidite deposits. However, observed turbidite hydrodynamics, and grain-size data from experimental and naturally occurring sandy turbidite deposits, indicate that T[sub n]-T[sub c] intervals of sandy turbidites are generally moderately well sorted, with low matrix content. Fluorescencemore » microscopy reveals that the carbonate fraction of these mixed siliciclastic-carbonate turbidites contains micritized skeletal grains and fusulinids, and algal peloids. These micritized grains and peloids were physically compacted and neomorphosed to form a carbonate pseudomatrix. Formation of carbonate pseudomatrix is analogous to formation of pseudomatrix in siliciclastic lithic sands, which includes crushing and recrystallization of lithic grains. Grain-size analysis of siliciclastic and slightly compacted carbonate grains indicates that these are moderately well sorted turbidite deposits with similar grain-size populations in both fractions. Lack of recognition of carbonate pseudomatrix could lead to erroneous interpretations of carbonate petrology. Identification of carbonate pseudomatrix is important to the study of mixed siliciclastic-carbonate gravity-flow deposits. This study demonstrates the value of fluorescence microscopy in the recognition of carbonate pseudomatrix.« less

The effects of sorting by aeolian processes on the geochemical characteristics of surface materials: a wind tunnel experiment

NASA Astrophysics Data System (ADS)

Wang, Xunming; Lang, Lili; Hua, Ting; Zhang, Caixia; Li, Hui

2018-03-01

The geochemical characteristics of aeolian and surface materials in potential source areas of dust are frequently employed in environmental reconstructions as proxies of past climate and as source tracers of aeolian sediments deposited in downwind areas. However, variations in the geochemical characteristics of these aeolian deposits that result from near-surface winds are currently poorly understood. In this study, we collected surface samples from the Ala Shan Plateau (a major potential dust source area in Central Asia) to determine the influence of aeolian processes on the geochemical characteristics of aeolian transported materials. Correlation analyses show that compared with surface materials, the elements in transported materials (e.g., Cu, As, Pb, Mn, Zn, Al, Ca, Fe, Ga, K, Mg, P, Rb, Co, Cr, Na, Nb, Si, and Zr) were subjected to significant sorting by aeolian processes, and the sorting also varied among different particle size fractions and elements. Variations in wind velocity were significantly correlated with the contents of Cr, Ga, Sr, Ca, Y, Nd, Zr, Nb, Ba, and Al, and with the Zr/Al, Zr/Rb, K/Ca, Sr/Ca, Rb/Sr, and Ca/Al ratios. Given the great variation in the geochemical characteristics of materials transported under different aeolian processes relative to those of the source materials, these results indicate that considerable uncertainty may be introduced to analyses by using surface materials to trace the potential source areas of aeolian deposits that accumulate in downwind areas.

Genomic Insights into Geothermal Spring Community Members using a 16S Agnostic Single-Cell Approach

NASA Astrophysics Data System (ADS)

Bowers, R. M.

2016-12-01

INSTUTIONS (ALL): DOE Joint Genome Institute, Walnut Creek, CA USA. Bigelow Laboratory for Ocean Sciences, East Boothbay, ME USA. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada. ABSTRACT BODY: With recent advances in DNA sequencing, rapid and affordable screening of single-cell genomes has become a reality. Single-cell sequencing is a multi-step process that takes advantage of any number of single-cell sorting techniques, whole genome amplification (WGA), and 16S rRNA gene based PCR screening to identify the microbes of interest prior to shotgun sequencing. However, the 16S PCR based screening step is costly and may lead to unanticipated losses of microbial diversity, as cells that do not produce a clean 16S amplicon are typically omitted from downstream shotgun sequencing. While many of the sorted cells that fail the 16S PCR step likely originate from poor quality amplified DNA, some of the cells with good WGA kinetics may instead represent bacteria or archaea with 16S genes that fail to amplify due to primer mis-matches or the presence of intervening sequences. Using cell material from Dewar Creek, a hot spring in British Columbia, we sequenced all sorted cells with good WGA kinetics irrespective of their 16S amplification success. We show that this high-throughput approach to single-cell sequencing (i) can reduce the overall cost of single-cell genome production, and (ii). may lead to the discovery of previously unknown branches on the microbial tree of life.

GGA1 regulates signal-dependent sorting of BACE1 to recycling endosomes, which moderates Aβ production

PubMed Central

Toh, Wei Hong; Chia, Pei Zhi Cheryl; Hossain, Mohammed Iqbal; Gleeson, Paul A.

2018-01-01

The diversion of the membrane-bound β-site amyloid precursor protein–(APP) cleaving enzyme (BACE1) from the endolysosomal pathway to recycling endosomes represents an important transport step in the regulation of amyloid beta (Aβ) production. However, the mechanisms that regulate endosome sorting of BACE1 are poorly understood. Here we assessed the transport of BACE1 from early to recycling endosomes and have identified essential roles for the sorting nexin 4 (SNX4)-mediated, signal-independent pathway and for a novel signal-mediated pathway. The signal-mediated pathway is regulated by the phosphorylation of the DXXLL-motif sequence DISLL in the cytoplasmic tail of BACE1. The phosphomimetic S498D BACE1 mutant was trafficked to recycling endosomes at a faster rate compared with wild-type BACE1 or the nonphosphorylatable S498A mutant. The rapid transit of BACE1 S498D from early endosomes was coupled with reduced levels of amyloid precursor protein processing and Aβ production, compared with the S498A mutant. We show that the adaptor, GGA1, and retromer are essential to mediate rapid trafficking of phosphorylated BACE1 to recycling endosomes. In addition, the BACE1 DISLL motif is phosphorylated and regulates endosomal trafficking, in primary neurons. Therefore, post-translational phosphorylation of DISLL enhances the exit of BACE1 from early endosomes, a pathway mediated by GGA1 and retromer, which is important in regulating Aβ production. PMID:29142073

Optical-electronic system for express analysis of mineral raw materials dressability by color sorting method

NASA Astrophysics Data System (ADS)

Alekhin, Artem A.; Gorbunova, Elena V.; Chertov, Aleksandr N.; Petuhova, Darya B.

2013-04-01

Due to the depletion of solid minerals ore reserves and the involvement in the production of the poor and refractory ores a process of continuous appreciation of minerals is going. In present time at the market of enrichment equipment are well represented optical sorters of various firms. All these sorters are essentially different from each other by parameters of productivity, classes of particles sizes for processed raw, nuances of decision algorithm, as well as by color model (RGB, YUV, HSB, etc.) chosen to describe the color of separating mineral samples. At the same time there is no dressability estimation method for mineral raw materials without direct semi-industrial test on the existing type of optical sorter, as well as there is no equipment realizing mentioned dressability estimation method. It should also be note the lack of criteria for choosing of one or another manufacturer (or type) of optical sorter. A direct consequence of this situation is the "opacity" of the color sorting method and the rejection of its potential customers. The proposed solution of mentioned problems is to develop the dressability estimation method, and to create an optical-electronic system for express analysis of mineral raw materials dressability by color sorting method. This paper has the description of structure organization and operating principles of experimental model optical-electronic system for express analysis of mineral raw material. Also in this work are represented comparison results of the proposed optical-electronic system and the real color sorter.

Effects of slope on the dynamics of dilute pyroclastic density currents from May 18th, 1980 Mt. St. Helens eruption

NASA Astrophysics Data System (ADS)

Bendana, S.; Self, S.; Dufek, J.

2012-12-01

The infamous, May 18th, 1980 eruption of Mt St Helens in the state of Washington produced several episodes of pyroclastic density currents (PDCs) including the initial lateral blast, which traveled nearly 30 km, and later PDCs, which filled in the area up to 8 km north of the volcano. The focus of this research is on the later PDCs, which differed from the lateral blast in that they have a higher particle concentration and filled in the topography up to 40 m. While the concentrated portions of the afternoon PDCs followed deep topographic drainages down the steep flanks of the volcano, the dilute overriding cloud partially decoupled to develop fully dilute, turbulent PDCs on the flanks of the volcano (Beeson, D.L. 1988. Proximal Flank Facies of the May 18, 1980 Ignimbrite: Mt. St. Helens, Washington.). The dilute PDCs deposited thin, cross-stratified and stratified pyroclastic deposits, known as the proximal bedded deposits, which differ greatly in depositional characteristics from the thick, massive, poorly-sorted, block-rich deposits associated with the more concentrated portions of the flow. We explore the influence of topography on the formation of these dilute currents and influence of slope on the currents transport and depositional mechanisms. The deposits on steeper slopes (>15°) are fines depleted relative to the proximal bedded deposits on shallower slopes (<15°). Bedform amplitude and wavelength increase with increasing slope, as does the occurrence of regressive dunes. Increasing slope causes an increase in flow velocity and thus an increase in flow turbulence. The fines depleted deposits suggest that fine ash elutriation is more efficient in flows with stronger turbulence. The longer wavelength and amplitudes suggest that bedform morphology is directly related to flow velocity, an important finding since the controls on bedform wavelength and amplitude in density stratified flows remains poorly constrained. The occurrence of regressive dunes, often interpreted as high flow-regime bedforms, on steeper slopes relative to progressive dunes on shallower slopes further attests to the control of velocity and flow regime on bedform morphology. Samples collected from recently exposed deposits and analyzed by grain size measurements, density analyses, and crystal morphoscopy studies further assess modes of origin and transport of dilute PDCs. The collected data will be used to validate numerical models that attempt to quantify the hazards of decoupled, dilute PDCs.

Drug delivery strategies for poorly water-soluble drugs.

PubMed

Fahr, Alfred; Liu, Xiangli

2007-07-01

The drug candidates coming from combinatorial chemistry research and/or the drugs selected from biologically based high-throughput screening are quite often very lipophilic, as these drug candidates exert their pharmacological action at or in biological membranes or membrane-associated proteins. This challenges drug delivery institutions in industry or academia to develop carrier systems for the optimal oral and parenteral administration of these drugs. To mention only a few of the challenges for this class of drugs: their oral bioavailability is poor and highly variable, and carrier development for parenteral administration is faced with problems, including the massive use of surface-active excipients for solubilisation. Formulation specialists are confronted with an even higher level of difficulties when these drugs have to be delivered site specifically. This article addresses the emerging formulation designs for delivering of poorly water-soluble drugs.

Contribution of Massive Stars to the Production of Neutron Capture Elements

NASA Astrophysics Data System (ADS)

Federman, Steven

2010-09-01

Elements beyond the Fe-peak must be synthesized through neutron-capture processes. With the aim of understanding the contribution of massive stars to the synthesis of neutron-capture elements during the current epoch, we propose an archival survey of interstellar arsenic, cadmium, tin, and lead. Nucleosynthesis via the weak slow process and the rapid process are the routes involving massive stars, while the main slow process arises from the evolution of low-mass stars. Ultraviolet lines for the dominant ions for each element will be used to extract interstellar abundances. The survey involves about forty sight lines, many of which are associated with regions of massive star formation shaped by core-collapse supernovae {SNe II}. The sample will increase the number of published determinations by factors of 2 to 5. HST spectra are the only means for determining the elemental abundances for this set of species in diffuse interstellar clouds. The survey contains directions that are both molecule poor and molecule rich, thereby enabling us to examine the overall level of depletion onto grains as a function of gas density. Complementary laboratory determinations of oscillator strengths will place the interstellar measurements on an absolute scale. The results from the proposed study will be combined with published interstellar abundances for other neutron capture elements and the suite of measurements will be compared to results from stars throughout the history of the Galaxy.

A Massive-born Neutron Star with a Massive White Dwarf Companion

NASA Astrophysics Data System (ADS)

Cognard, Ismaël; Freire, Paulo C. C.; Guillemot, Lucas; Theureau, Gilles; Tauris, Thomas M.; Wex, Norbert; Graikou, Eleni; Kramer, Michael; Stappers, Benjamin; Lyne, Andrew G.; Bassa, Cees; Desvignes, Gregory; Lazarus, Patrick

2017-08-01

We report on the results of a 4 year timing campaign of PSR J2222-0137, a 2.44 day binary pulsar with a massive white dwarf (WD) companion, with the Nançay, Effelsberg, and Lovell radio telescopes. Using the Shapiro delay for this system, we find a pulsar mass m p = 1.76 ± 0.06 M ⊙ and a WD mass m c = 1.293 ± 0.025 M ⊙. We also measure the rate of advance of periastron for this system, which is marginally consistent with the general relativity prediction for these masses. The short lifetime of the massive WD progenitor star led to a rapid X-ray binary phase with little (< 10-2 M ⊙) mass accretion onto the neutron star; hence, the current pulsar mass is, within uncertainties, its birth mass, which is the largest measured to date. We discuss the discrepancy with previous mass measurements for this system; we conclude that the measurements presented here are likely to be more accurate. Finally, we highlight the usefulness of this system for testing alternative theories of gravity by tightly constraining the presence of dipolar radiation. This is of particular importance for certain aspects of strong-field gravity, like spontaneous scalarization, since the mass of PSR J2222-0137 puts that system into a poorly tested parameter range.

Evaluation de differentes strategies de demantelement de la carcasse d'un avion

NASA Astrophysics Data System (ADS)

Sainte-Beuve, Damien

At the time when sustainable development of our environment and our society become more and more considered, the issue of the end of life of aircraft arises. With the tightening of regulations and policies to reduce operating costs, aircraft are increasingly removed from service before the end of their operational life. However, the regulations do not only affect the operational life of the aircraft. In the future, there are great chances that the aircraft end of life will be also regulated as the car end of life. Nowadays some deserts serve as a tomb for airplanes skeletons, but what will happen in a few years? Recycling the skeleton is not an operation with very high added value, such as reselling used parts, however intelligent sorting and facing the market can increase the gain. At the level of the aircraft life cycle, recycling its materials reduces its overall impact, and also helps to reduce pollution and land use, even if the main impacts generated by planes are created during the use phase. Similarly, if the recyclability of the aircraft is studied at the source, that is to say in its design, this will help to reduce the use of non-recyclable materials and dangerous compound. The greatest difficulty for recycling aircraft skeletons is that different materials are mixed and attached to each other. Through a thermodynamic study we showed what concentrations of alloying elements in a molten bath could be reduced. By using the Gibbs free energy, we evaluated some of the reactions taking place in a molten bath of aluminum during the injection of oxygen, boron, and chlorine. We focused on the reactions forming dual elements compounds, such as lithium oxide or magnesium chloride. We have shown that the six elements able to react when these reactions occurs are the lithium, the magnesium, the nickel, the titanium, the vanadium and the zirconium. It is necessary to remember that the impurities for alloys used in the aerospace industry are lower and especially those of iron and silicon. The major problem of recycling of aluminum alloys is that some attachments are made from steels, and are not separated from aluminum. Iron binds too easily with aluminum and removing it is expensive and complicated. The separation of these disturbing parts can be performed before or after shredding parts. Present sorting pathways already permit at a certain scale, to make this type of sorting. Adding some type of sorting, such as the LIBS or the use of dry ice blasting can help to increase the purity of the alloys obtained. On the other hand, the number of different aircraft being relatively small, it is possible to develop specific methods to recycle specific aircraft and this according to the technologies available at the end of life of the aircraft. In this context we have studied the wing of a Canadair Regional Jet 200. After characterizing more than 80% by weight of the wing, we looked at determining areas to maximize the alloys recycled in a close loop. We developed a method to create homogenous areas with the data we collected. We have shown that the removal of certain parts, sometimes massive such as landing gear support can reduce the final impurity. In addition, the more massive they are, the more their withdrawal is interesting because while improving the quality of the whole mix, the parts removed are recoverable their self. Unfortunately, for the final work to provide a real gain it is necessary that the data is of high quality, and numerous. This rule is even truer if we apply an algorithm to create homogeneous areas. This algorithm takes into account the market demands, to create areas satisfying this demand. With three-dimensional data, the algorithm is able to take into account the actual average concentration of elements present, and all the parts left during the dismantling of the plane. Due to the complexity of assemblies, neither the post-milling nor the pre-shredding treatment is perfect. This is why it is interesting and productive to combine them. Sorting pre-shredding can save and facilitate the post-shredding.

A Lower Permian sandwave-containing shelf sequence exposed at Zungwini Mountain, Republic of South Africa

NASA Astrophysics Data System (ADS)

Smith, A. M.

1989-08-01

As a result of railway excavations the Pietermaritzburg Shale-Vryheid Formation transition is spectacularly exposed on the southern slope of Zungwini Mountain. Nine facies and three facies associations are recognised. Deposition occurred in a palaeoshelf and offshore setting. The reconstructed coastline was SW-NE with land to the northwest. The inner shelf was tide- and the outer-shelf storm-influenced. Fluvial input supplied sediment which was reworked into flood-tidal sandwaves, probably within the confines of an estuary. A rising sea level brought the sandwaves into the realm of a more distal, coast-parallel, storm-tidal current regime where reworking of the sediment occurred. Intense storm-augmented tidal currents swept some of the better-sorted material seaward to be deposited as storm layers in the inner and outer shelf. These same currents formed the low-density turbidites and sediment plumes from which the offshore argillaceous deposits were formed. The shelf edge poorly sorted rhythmite facies may have developed from sediment flushed out of the rivers during flood or from the flood-tidal sandwave system as a result of exceptional coastal storms.

Reconsidering the Relationship between Air Pollution and Deprivation

PubMed Central

Bailey, Nick; Dong, Guanpeng; Minton, Jon; Pryce, Gwilym

2018-01-01

This paper critically examines the relationship between air pollution and deprivation. We argue that focusing on a particular economic or social model of urban development might lead one to erroneously expect all cities to converge towards a particular universal norm. A naive market sorting model, for example, would predict that poor households will eventually be sorted into high pollution areas, leading to a positive relationship between air pollution and deprivation. If, however, one considers a wider set of theoretical perspectives, the anticipated relationship between air pollution and deprivation becomes more complex and idiosyncratic. Specifically, we argue the relationship between pollution and deprivation can only be made sense of by considering processes of risk perception, path dependency, gentrification and urbanization. Rather than expecting all areas to eventually converge to some universal norm, we should expect the differences in the relationship between air pollution and deprivation across localities to persist. Mindful of these insights, we propose an approach to modeling which does not impose a geographically fixed relationship. Results for Scotland reveal substantial variations in the observed relationships over space and time, supporting our argument. PMID:29596380

Sedimentological Characterization of a Deepwater Methane Hydrate Reservoir in Green Canyon 955, Northern Gulf of Mexico

NASA Astrophysics Data System (ADS)

Meazell, K.; Flemings, P. B.

2017-12-01

Grain size is a controlling factor of hydrate saturation within a Pleistocene channel-levee system investigated by the UT-GOM2-1 expedition within the deepwater northern Gulf of Mexico. Laser diffraction and settling experiments conducted on sediments from 413-440 meters below the seafloor reveal the presence of two interbedded lithologic units, identified as a silty sand and a clayey silt, according Shepard's classification system. The sand-rich lithofacies has low density and high p-wave velocity, suggesting a high degree of hydrate saturation. Conversely, the clay and silt dominated lithofacies is characterized by a higher density and low p-wave velocity, suggesting low hydrate saturation. The sand-rich lithofacies is well-sorted and displays abundant ripple lamination, indicative of deposition within a high-energy environment. The clayey-silt is poorly-sorted and lacks sedimentary structures. The two lithofacies are interbedded throughout the reservoir unit; however, the relative abundance of the sand-rich lithofacies increases with depth, suggesting a potential decrease in flow energy or sediment flux over time, resulting in the most favorable reservoir properties near the base of the unit.

Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria.

PubMed

Popov-Celeketić, Dusan; Mapa, Koyeli; Neupert, Walter; Mokranjac, Dejana

2008-05-21

The TIM23 (translocase of the mitochondrial inner membrane) complex mediates translocation of preproteins across and their insertion into the mitochondrial inner membrane. How the translocase mediates sorting of preproteins into the two different subcompartments is poorly understood. In particular, it is not clear whether association of two operationally defined parts of the translocase, the membrane-integrated part and the import motor, depends on the activity state of the translocase. We established conditions to in vivo trap the TIM23 complex in different translocation modes. Membrane-integrated part of the complex and import motor were always found in one complex irrespective of whether an arrested preprotein was present or not. Instead, we detected different conformations of the complex in response to the presence and, importantly, the type of preprotein being translocated. Two non-essential subunits of the complex, Tim21 and Pam17, modulate its activity in an antagonistic manner. Our data demonstrate that the TIM23 complex acts as a single structural and functional entity that is actively remodelled to sort preproteins into different mitochondrial subcompartments.

Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria

PubMed Central

Popov-Čeleketić, Dus̆an; Mapa, Koyeli; Neupert, Walter; Mokranjac, Dejana

2008-01-01

The TIM23 (translocase of the mitochondrial inner membrane) complex mediates translocation of preproteins across and their insertion into the mitochondrial inner membrane. How the translocase mediates sorting of preproteins into the two different subcompartments is poorly understood. In particular, it is not clear whether association of two operationally defined parts of the translocase, the membrane-integrated part and the import motor, depends on the activity state of the translocase. We established conditions to in vivo trap the TIM23 complex in different translocation modes. Membrane-integrated part of the complex and import motor were always found in one complex irrespective of whether an arrested preprotein was present or not. Instead, we detected different conformations of the complex in response to the presence and, importantly, the type of preprotein being translocated. Two non-essential subunits of the complex, Tim21 and Pam17, modulate its activity in an antagonistic manner. Our data demonstrate that the TIM23 complex acts as a single structural and functional entity that is actively remodelled to sort preproteins into different mitochondrial subcompartments. PMID:18418384

Overlapping contributions of Msh1p and putative recombination proteins Cce1p, Din7p, and Mhr1p in large-scale recombination and genome sorting events in the mitochondrial genome of Saccharomyces cerevisiae.

PubMed

Mookerjee, Shona A; Sia, Elaine A

2006-03-20

The mechanisms that govern mutation avoidance in the mitochondrial genome, though believed to be numerous, are poorly understood. The identification of individual genes has implicated mismatch repair and several recombination pathways in maintaining the fidelity and structural stability of mitochondrial DNA. However, the majority of genes in these pathways have not been identified and the interactions between different pathways have not been extensively studied. Additionally, the multicopy presence of the mitochondrial genome affects the occurrence and persistence of mutant phenotypes, making mitochondrial DNA transmission and sorting important factors affecting mutation accumulation. We present new evidence that the putative recombination genes CCE1, DIN7, and MHR1 have overlapping function with the mismatch repair homolog MSH1 in point mutation avoidance and suppression of aberrant recombination events. In addition, we demonstrate a novel role for Msh1p in mtDNA transmission, a role not predicted by studies of its nuclear homologs.

Quality changes in macadamia kernel between harvest and farm-gate.

PubMed

Walton, David A; Wallace, Helen M

2011-02-01

Macadamia integrifolia, Macadamia tetraphylla and their hybrids are cultivated for their edible kernels. After harvest, nuts-in-shell are partially dried on-farm and sorted to eliminate poor-quality kernels before consignment to a processor. During these operations, kernel quality may be lost. In this study, macadamia nuts-in-shell were sampled at five points of an on-farm postharvest handling chain from dehusking to the final storage silo to assess quality loss prior to consignment. Shoulder damage, weight of pieces and unsound kernel were assessed for raw kernels, and colour, mottled colour and surface damage for roasted kernels. Shoulder damage, weight of pieces and unsound kernel for raw kernels increased significantly between the dehusker and the final silo. Roasted kernels displayed a significant increase in dark colour, mottled colour and surface damage during on-farm handling. Significant loss of macadamia kernel quality occurred on a commercial farm during sorting and storage of nuts-in-shell before nuts were consigned to a processor. Nuts-in-shell should be dried as quickly as possible and on-farm handling minimised to maintain optimum kernel quality. 2010 Society of Chemical Industry.

GGA3 Functions as a Switch to Promote Met Receptor Recycling, Essential for Sustained ERK and Cell Migration

PubMed Central

Parachoniak, Christine Anna; Luo, Yi; Abella, Jasmine Vanessa; Keen, James H.; Park, Morag

2011-01-01

Summary Cells are dependent on correct sorting of activated receptor tyrosine kinases (RTKs) for the outcome of growth factor signaling. Upon activation, RTKs are coupled through the endocytic machinery for degradation, or recycled to the cell surface. However, the molecular mechanisms governing RTK recycling are poorly understood. Here, we show that Golgi-localized gamma-ear containing Arf-binding protein 3 (GGA3) interacts selectively with the Met/Hepatocyte Growth Factor RTK when stimulated, to sort it for recycling in association with “gyrating”-clathrin. GGA3 loss abrogates Met recycling from a Rab4 endosomal subdomain, resulting in pronounced trafficking of Met towards degradation. Decreased Met recycling attenuates ERK activation and cell migration. Met recycling, sustained ERK activation and migration require interaction of GGA3 with Arf6 and an unexpected association with the Crk adaptor. The data show that GGA3 defines an active recycling pathway and support a broader role for GGA3-mediated cargo selection in targeting receptors destined for recycling. PMID:21664574

Reconsidering the Relationship between Air Pollution and Deprivation.

PubMed

Bailey, Nick; Dong, Guanpeng; Minton, Jon; Pryce, Gwilym

2018-03-29

This paper critically examines the relationship between air pollution and deprivation. We argue that focusing on a particular economic or social model of urban development might lead one to erroneously expect all cities to converge towards a particular universal norm. A naive market sorting model, for example, would predict that poor households will eventually be sorted into high pollution areas, leading to a positive relationship between air pollution and deprivation. If, however, one considers a wider set of theoretical perspectives, the anticipated relationship between air pollution and deprivation becomes more complex and idiosyncratic. Specifically, we argue the relationship between pollution and deprivation can only be made sense of by considering processes of risk perception, path dependency, gentrification and urbanization. Rather than expecting all areas to eventually converge to some universal norm, we should expect the differences in the relationship between air pollution and deprivation across localities to persist. Mindful of these insights, we propose an approach to modeling which does not impose a geographically fixed relationship. Results for Scotland reveal substantial variations in the observed relationships over space and time, supporting our argument.

Neutron-Capture Elements in Very Metal-Poor Halo Stars

NASA Astrophysics Data System (ADS)

French, R. S.; Sneden, C.; Cowan, J. J.; Lawler, J. E.; Primas, F.; Beers, T. C.; Truran, J. W.

2000-05-01

Abundances of the most massive stable elements (Os -> Pb or 76 <= Z <= 82) in metal-poor stars can provide crucial information about the so-called ``third neutron-capture peak,'' and are critical to the radioactive-dating technique that uses unstable thorium and uranium as chronometers. As the relevant transitions occur in the UV and are inaccessable to ground-based telescopes, we have obtained high resolution (R ~= 30,000) UV spectra of 10 very metal-poor (--3.0 <= [Fe/H] <= --1.4) halo giants using the Space Telescope Imaging Spectrograph (STIS) aboard the Hubble Space Telescope. Using iterative spectrum synthesis techniques, we derive abundances for some of these heavy elements. We compare our abundances to those predicted for very metal-poor stars based on a scaled solar system rapid-process (production in rapid neutron-capture synthesis events, such as occurs during supernovae explosions). This research is supported by NASA STScI grant GO-08342 and NSF grants AST-9618364 to C.S. and AST-9618332 to J.J.C.

Impact of time to endovascular reperfusion on outcome differs according to the involvement of the proximal MCA territory.

PubMed

Hedderich, Dennis M; Boeckh-Behrens, Tobias; Friedrich, Benjamin; Wiestler, Benedikt; Wunderlich, Silke; Zimmer, Claus; Fischer, Urs; Kleine, Justus F; Kaesmacher, Johannes

2018-06-01

The time interval between symptom onset and reperfusion is a major determinant of the benefit of endovascular therapy (ET) and patients' outcome. The impact of time may be attenuated in patients with robust collaterals. However, not all regions in the middle cerebral artery (MCA) territory have access to collaterals. To evaluate if the involvement of the poorly collateralized proximal MCA territory has an impact on the degree of time dependency of patients' outcome. Patients with MCA occlusions treated with ET and involvement/sparing of the proximal striatocapsular MCA territory (SC+/SC-, each n=97) were matched according to their symptom onset to reperfusion times (SORTs). Correlation and impact of time on outcome was evaluated with strata of SC+/SC- using multivariate logistic regression models (LRMs), including interaction terms. Discharge National Institute of Health Stroke Scale (NIHSS-DIS) score <5 and discharge modified Rankin Scale (mRS-DIS) score ≤2 were prespecified outcome measures. A stronger correlation between all outcome measures (NIHSS-DIS/ΔNIHSS/mRS-DIS) and SORTs was found for SC+ patients than for SC-patients. SORTs were significant variables in LRMs for mRS-DIS score ≤2 and NIHSS-DIS score <5 in SC+ but not in SC- patients. Interaction of SC+ and SORTs was significant in LRMs for both endpoints. Time dependency of outcome after ET is more pronounced if parts of the proximal MCA territory are affected. This may reflect the lack of collateralization in the striatocapsular region and a more stringent cell death with time. If confirmed, this finding may affect the selection of patients based on different time windows according to the territory at risk. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Genetic profiling of putative breast cancer stem cells from malignant pleural effusions.

PubMed

Tiran, Verena; Stanzer, Stefanie; Heitzer, Ellen; Meilinger, Michael; Rossmann, Christopher; Lax, Sigurd; Tsybrovskyy, Oleksiy; Dandachi, Nadia; Balic, Marija

2017-01-01

A common symptom during late stage breast cancer disease is pleural effusion, which is related to poor prognosis. Malignant cells can be detected in pleural effusions indicating metastatic spread from the primary tumor site. Pleural effusions have been shown to be a useful source for studying metastasis and for isolating cells with putative cancer stem cell (CSC) properties. For the present study, pleural effusion aspirates from 17 metastatic breast cancer patients were processed to propagate CSCs in vitro. Patient-derived aspirates were cultured under sphere forming conditions and isolated primary cultures were further sorted for cancer stem cell subpopulations ALDH1+ and CD44+CD24-/low. Additionally, sphere forming efficiency of CSC and non-CSC subpopulations was determined. In order to genetically characterize the different tumor subpopulations, DNA was isolated from pleural effusions before and after cell sorting, and compared with corresponding DNA copy number profiles from primary tumors or bone metastasis using low-coverage whole genome sequencing (SCNA-seq). In general, unsorted cells had a higher potential to form spheres when compared to CSC subpopulations. In most cases, cell sorting did not yield sufficient cells for copy number analysis. A total of five from nine analyzed unsorted pleura samples (55%) showed aberrant copy number profiles similar to the respective primary tumor. However, most sorted subpopulations showed a balanced profile indicating an insufficient amount of tumor cells and low sensitivity of the sequencing method. Finally, we were able to establish a long term cell culture from one pleural effusion sample, which was characterized in detail. In conclusion, we confirm that pleural effusions are a suitable source for enrichment of putative CSC. However, sequencing based molecular characterization is impeded due to insufficient sensitivity along with a high number of normal contaminating cells, which are masking genetic alterations of rare cancer (stem) cells.

Are residents of downtown Toronto influenced by their urban neighbourhoods? Using concept mapping to examine neighbourhood characteristics and their perceived impact on self-rated mental well-being.

PubMed

Sheppard, Amanda J; Salmon, Christina; Balasubramaniam, Priya; Parsons, Janet; Singh, Gita; Jabbar, Amina; Zaidi, Qamar; Scott, Allison; Nisenbaum, Rosane; Dunn, Jim; Ramsay, Jason; Haque, Nasim; O'Campo, Patricia

2012-08-03

There is ample evidence that residential neighbourhoods can influence mental well-being (MWB), with most studies relying on census or similar data to characterize communities. Few studies have actively investigated local residents' perceptions. Concept mapping was conducted with residents from five Toronto neighbourhoods representing low income and non-low income socio-economic groups. These residents participated in small groups and attended two sessions per neighbourhood. The first session (brainstorming) generated neighbourhood characteristics that residents felt influenced their MWB. A few weeks later, participants returned to sort these neighbourhood characteristics and rate their relative importance in affecting residents' 'good' and 'poor' MWB. The data from the sorting and rating groups were analyzed to generate conceptual maps of neighbourhood characteristics that influence MWB. While agreement existed on factors influencing poor MWB (regardless of neighbourhood, income, gender and age), perceptions related to factors affecting good MWB were more varied. For example, women were more likely to rank physical beauty of their neighbourhood and range of services available as more important to good MWB, while men were more likely to cite free access to computers/internet and neighbourhood reputation as important. Low-income residents emphasized aesthetic attributes and public transportation as important to good MWB, while non-low-income residents rated crime, negative neighbourhood environment and social concerns as more important contributors to good MWB. These findings contribute to the emerging literature on neighbourhoods and MWB, and inform urban planning in a Canadian context.

A comparative analysis of the distribution, composition and geochemistry of surface sediments in the Linthipe and Songwe River Deltas of Lake Malawi

NASA Astrophysics Data System (ADS)

Dolozi, Michael B.; Kalindekafe, Leonard S. N.; Ngongondo, Cosmo; Dulanya, Zuze

2011-05-01

The Linthipe and Songwe River Deltas are found to the extreme southern and northern parts of Lake Malawi respectively within the East African Rift System. They occur in contrasting tectonic and climatic settings of the rift-valley half-graben structure. The sub-aqueous part of the Songwe Delta consists of relatively finer grained sediments than the Linthipe but is relatively poorly sorted. The composition of sediments within the Songwe Delta shows significant amounts of sedimentary and volcanic lithic fragments which are lacking in the Linthipe. On the other hand, ferromanganese nodules were recovered in the Linthipe Delta at water depths of 80-100 m but were not recovered in Songwe Delta at similar water depths. The finer grained facies of the Songwe Delta suggests a more prograded delta than the Linthipe. However, its poorly sorted sediments are most likely due to the heterogeneous geology of the source area; higher and faster depositional rates due to climatic influences. The lack of Ferromangenese nodules in the Songwe Delta is probably due to the sand-mud facies boundary which occurs at shallower depth. The higher proportion of Total Organic Carbon (TOC) in higher the Linthipe Delta is probably related to high rates of environmental degradation such as deforestation and agricultural activities in the riparian catchment basin. This is in contrast to the Songwe catchment basin where the levels of anthropogenic disturbance are less and climate, geomorphology and the heterogenous character of the source rocks seems to play a major role in the sedimentation processes.

Shallow-water gaseohydrothermal plume studies after massive eruption at Panarea, Aeolian Islands, Italy

NASA Astrophysics Data System (ADS)

Tudino, T.; Bortoluzzi, G.; Aliani, S.

2014-03-01

Marine water dynamics in the near field of a massive gas eruption near Panarea (Aeolian Islands volcanic arc, SE Tyrrhenian Sea) is described. ADCP current-meters were deployed during the paroxysmal phase in 2002 and 2003 a few meters from the degassing vent, recording day-long time series. Datasets were sorted to remove errors and select good quality ensembles over the entire water column. Standard deviation of error velocity was considered a proxy for inhomogeneous velocity fields over beams. Time series intervals had been selected when the basic ADCP assumptions were fulfilled and random errors minimized. Backscatter data were also processed to identify bubbles in the water column with the aim of locating bubble-free ensembles. Reliable time series are selected combining these data. Two possible scenarios have been described: firstly, a highly dynamic situation with visible surface diverging rings of waves, entrainment on the lower part of the gas column, detrainment in the upper part and a stagnation line (SL) at mid depth where currents were close to zero and most of the gas bubbles spread laterally; secondly, a less dynamic situation with water entraining into the gas plume at all depths and no surface rings of diverging waves. Reasons for these different dynamics may be ascribed to changes in gas fluxes (one order of magnitude higher in 2002). Description of SL is important to quantify its position in the water column and timing for entrainment-detrainment, and it can be measured by ADCP and calculated from models.

Design of multiple sequence alignment algorithms on parallel, distributed memory supercomputers.

PubMed

Church, Philip C; Goscinski, Andrzej; Holt, Kathryn; Inouye, Michael; Ghoting, Amol; Makarychev, Konstantin; Reumann, Matthias

2011-01-01

The challenge of comparing two or more genomes that have undergone recombination and substantial amounts of segmental loss and gain has recently been addressed for small numbers of genomes. However, datasets of hundreds of genomes are now common and their sizes will only increase in the future. Multiple sequence alignment of hundreds of genomes remains an intractable problem due to quadratic increases in compute time and memory footprint. To date, most alignment algorithms are designed for commodity clusters without parallelism. Hence, we propose the design of a multiple sequence alignment algorithm on massively parallel, distributed memory supercomputers to enable research into comparative genomics on large data sets. Following the methodology of the sequential progressiveMauve algorithm, we design data structures including sequences and sorted k-mer lists on the IBM Blue Gene/P supercomputer (BG/P). Preliminary results show that we can reduce the memory footprint so that we can potentially align over 250 bacterial genomes on a single BG/P compute node. We verify our results on a dataset of E.coli, Shigella and S.pneumoniae genomes. Our implementation returns results matching those of the original algorithm but in 1/2 the time and with 1/4 the memory footprint for scaffold building. In this study, we have laid the basis for multiple sequence alignment of large-scale datasets on a massively parallel, distributed memory supercomputer, thus enabling comparison of hundreds instead of a few genome sequences within reasonable time.

A Clue to the Extent of Convective Mixing Inside Massive Stars: The Surface Hydrogen Abundances of Luminous Blue Variables and Hydrogen-Poor Wolf-Rayet Stars

NASA Technical Reports Server (NTRS)

Stothers, Richard B.; Chin, Chao-wen

1999-01-01

Interior layers of stars that have been exposed by surface mass loss reveal aspects of their chemical and convective histories that are otherwise inaccessible to observation. It must be significant that the surface hydrogen abundances of luminous blue variables (LBVs) show a remarkable uniformity, specifically X(sub surf) = 0.3 - 0.4, while those of hydrogen-poor Wolf-Rayet (WN) stars fall, almost without exception, below these values, ranging down to X(sub surf) = 0. According to our stellar model calculations, most LBVs are post-red-supergiant objects in a late blue phase of dynamical instability, and most hydrogen-poor WN stars are their immediate descendants. If this is so, stellar models constructed with the Schwarzschild (temperature-gradient) criterion for convection account well for the observed hydrogen abundances, whereas models built with the Ledoux (density-gradient) criterion fail. At the brightest luminosities, the observed hydrogen abundances of LBVs are too large to be explained by any of our highly evolved stellar models, but these LBVs may occupy transient blue loops that exist during an earlier phase of dynamical instability when the star first becomes a yellow supergiant. Independent evidence concerning the criterion for convection, which is based mostly on traditional color distributions of less massive supergiants on the Hertzsprung-Russell diagram, tends to favor the Ledoux criterion. It is quite possible that the true criterion for convection changes over from something like the Ledoux criterion to something like the Schwarzschild criterion as the stellar mass increases.

Denni Algorithm An Enhanced Of SMS (Scan, Move and Sort) Algorithm

NASA Astrophysics Data System (ADS)

Aprilsyah Lubis, Denni; Salim Sitompul, Opim; Marwan; Tulus; Andri Budiman, M.

2017-12-01

Sorting has been a profound area for the algorithmic researchers, and many resources are invested to suggest a more working sorting algorithm. For this purpose many existing sorting algorithms were observed in terms of the efficiency of the algorithmic complexity. Efficient sorting is important to optimize the use of other algorithms that require sorted lists to work correctly. Sorting has been considered as a fundamental problem in the study of algorithms that due to many reasons namely, the necessary to sort information is inherent in many applications, algorithms often use sorting as a key subroutine, in algorithm design there are many essential techniques represented in the body of sorting algorithms, and many engineering issues come to the fore when implementing sorting algorithms., Many algorithms are very well known for sorting the unordered lists, and one of the well-known algorithms that make the process of sorting to be more economical and efficient is SMS (Scan, Move and Sort) algorithm, an enhancement of Quicksort invented Rami Mansi in 2010. This paper presents a new sorting algorithm called Denni-algorithm. The Denni algorithm is considered as an enhancement on the SMS algorithm in average, and worst cases. The Denni algorithm is compared with the SMS algorithm and the results were promising.

Prospects for the U.S. energy and refining industries: Markets, profitability and key drivers for change

DOE Office of Scientific and Technical Information (OSTI.GOV)

Burke, B.F.

The US refining industry has experienced an extended period of change covering the past 20 years. Growing regulatory requirements, combined with shifting market characteristics, have resulted in massive investments and significant and ongoing structural change. Despite excellent capacity utilization, recent profitability has been poor. Industry psychology can be described as depressed, with honest concern about the long-term attractiveness of domestic refining as an area for continued participation and investment. This paper provides an overview of how the industry arrived at these levels of poor profitability, examines the current situation and future drivers, and presents Chem Systems` views on the outlookmore » for domestic refining.« less

Massive prolapsed haemorrhoids managed by ablation and correction in a poor resourced area

PubMed Central

Weledji, Elroy Patrick; Enow Orock, George; Aminde, Leopold

2013-01-01

More recently some patients with rectal mucosal prolapse and obstructive defaecation have been treated with the procedure for prolapse and haemorrhoids. We report a case of symptomatic chronic circumferentially prolapsed haemorrhoids that had several failed attempts at surgical repair. This was finally managed by ablation and correction of the associated rectal mucosal prolapse by a modified ‘Delorme's procedure akin to a stapled anopexy. PMID:24968428

Rmax: A systematic approach to evaluate instrument sort performance using center stream catch☆

PubMed Central

Riddell, Andrew; Gardner, Rui; Perez-Gonzalez, Alexis; Lopes, Telma; Martinez, Lola

2015-01-01

Sorting performance can be evaluated with regard to Purity, Yield and/or Recovery of the sorted fraction. Purity is a check on the quality of the sample and the sort decisions made by the instrument. Recovery and Yield definitions vary with some authors regarding both as how efficient the instrument is at sorting the target particles from the original sample, others distinguishing Recovery from Yield, where the former is used to describe the accuracy of the instrument’s sort count. Yield and Recovery are often neglected, mostly due to difficulties in their measurement. Purity of the sort product is often cited alone but is not sufficient to evaluate sorting performance. All of these three performance metrics require re-sampling of the sorted fraction. But, unlike Purity, calculating Yield and/or Recovery calls for the absolute counting of particles in the sorted fraction, which may not be feasible, particularly when dealing with rare populations and precious samples. In addition, the counting process itself involves large errors. Here we describe a new metric for evaluating instrument sort Recovery, defined as the number of particles sorted relative to the number of original particles to be sorted. This calculation requires only measuring the ratios of target and non-target populations in the original pre-sort sample and in the waste stream or center stream catch (CSC), avoiding re-sampling the sorted fraction and absolute counting. We called this new metric Rmax, since it corresponds to the maximum expected Recovery for a particular set of instrument parameters. Rmax is ideal to evaluate and troubleshoot the optimum drop-charge delay of the sorter, or any instrument related failures that will affect sort performance. It can be used as a daily quality control check but can be particularly useful to assess instrument performance before single-cell sorting experiments. Because we do not perturb the sort fraction we can calculate Rmax during the sort process, being especially valuable to check instrument performance during rare population sorts. PMID:25747337

Interpretation of massive sandstones in ephemeral fluvial settings: A case study from the Upper Candelária Sequence (Upper Triassic, Paraná Basin, Brazil)

NASA Astrophysics Data System (ADS)

Horn, Bruno Ludovico Dihl; Goldberg, Karin; Schultz, Cesar Leandro

2018-01-01

Ephemeral rivers display a wide range of upper- and lower-flow regime structures due to great flow-velocity changes during the floods. The development of flow structures in these setting is yet to be understood, especially in the formation of thick, massive sandstones. The Upper Triassic of Southern Gondwana was marked by a climate with great seasonal changes, yet there is no description of river systems with seasonal characteristics in Southern Gondwana. This work aims to characterize a ephemeral alluvial system of the Upper Triassic of the Paraná Basin. The characteristics of the deposits are discussed in terms of depositional processes through comparison with similar deposits from literature, flow characteristics and depositional signatures compared to flume experiments. The alluvial system is divided in four facies associations: (1) channels with wanning fill, characterized by low width/thickness ratio, tabular bodies, scour-and-fill structures with upper- and lower-flow regime bedforms; (2) channels with massive fill, characterized by low w/t ratio, sheet-like bodies, scour-and-fill structures with massive sandstones; (3) proximal sheetfloods, characterized by moderate w/t ratio, sheet-like bodies with upper- and lower-flow regime bedforms and (4) distal sheetfloods, characterized by high w/t ratio, sheet-like bodies with lower-flow regime bedforms. Evidence for the seasonal reactivation of the riverine system includes the scarcity of well-developed macroforms and presence of in-channel mudstones, thick intraformational conglomerates, and the occurrence of well- and poorly-preserved vertebrate bones in the same beds. The predominantly massive sandstones indicate deposition from a hyperconcentrated flow during abrupt changes in flow speed, caused by de-confinement or channel avulsion, whereas turbulent portions of the flow formed the upper- and lower-flow regime bedforms after the deposition of the massive layers. The upper portion of the Candelária Sequence records a good example of strongly ephemeral alluvial systems, where the predominance of massive sandstones is a particular characteristic.

Molecular dissection of Phaseolus vulgaris polygalacturonase-inhibiting protein 2 reveals the presence of hold/release domains affecting protein trafficking toward the cell wall

PubMed Central

De Caroli, Monica; Lenucci, Marcello S.; Manualdi, Francesca; Dalessandro, Giuseppe; De Lorenzo, Giulia; Piro, Gabriella

2015-01-01

The plant endomembrane system is massively involved in the synthesis, transport and secretion of cell wall polysaccharides and proteins; however, the molecular mechanisms underlying trafficking toward the apoplast are largely unknown. Besides constitutive, the existence of a regulated secretory pathway has been proposed. A polygalacturonase inhibitor protein (PGIP2), known to move as soluble cargo and reach the cell wall through a mechanism distinguishable from default, was dissected in its main functional domains (A, B, C, D), and C sub-fragments (C1–10), to identify signals essential for its regulated targeting. The secretion patterns of the fluorescent chimeras obtained by fusing different PGIP2 domains to the green fluorescent protein (GFP) were analyzed. PGIP2 N-terminal and leucine-rich repeat domains (B and C, respectively) seem to operate as holding/releasing signals, respectively, during PGIP2 transit through the Golgi. The B domain slows down PGIP2 secretion by transiently interacting with Golgi membranes. Its depletion leads, in fact, to the secretion via default (Sp2-susceptible) of the ACD-GFP chimera faster than PGIP2. Depending on its length (at least the first 5 leucine-rich repeats are required), the C domain modulates B interaction with Golgi membranes allowing the release of chimeras and their extracellular secretion through a Sp2 independent pathway. The addition of the vacuolar sorting determinant Chi to PGIP2 diverts the path of the protein from cell wall to vacuole, suggesting that C domain is a releasing rather than a cell wall sorting signal. PMID:26379688

THE VERY MASSIVE STAR CONTENT OF THE NUCLEAR STAR CLUSTERS IN NGC 5253

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, L. J.; Crowther, P. A.; Calzetti, D.

2016-05-20

The blue compact dwarf galaxy NGC 5253 hosts a very young starburst containing twin nuclear star clusters, separated by a projected distance of 5 pc. One cluster (#5) coincides with the peak of the H α emission and the other (#11) with a massive ultracompact H ii region. A recent analysis of these clusters shows that they have a photometric age of 1 ± 1 Myr, in apparent contradiction with the age of 3–5 Myr inferred from the presence of Wolf-Rayet features in the cluster #5 spectrum. We examine Hubble Space Telescope ultraviolet and Very Large Telescope optical spectroscopy ofmore » #5 and show that the stellar features arise from very massive stars (VMSs), with masses greater than 100 M {sub ⊙}, at an age of 1–2 Myr. We further show that the very high ionizing flux from the nuclear clusters can only be explained if VMSs are present. We investigate the origin of the observed nitrogen enrichment in the circumcluster ionized gas and find that the excess N can be produced by massive rotating stars within the first 1 Myr. We find similarities between the NGC 5253 cluster spectrum and those of metal-poor, high-redshift galaxies. We discuss the presence of VMSs in young, star-forming galaxies at high redshift; these should be detected in rest-frame UV spectra to be obtained with the James Webb Space Telescope . We emphasize that population synthesis models with upper mass cutoffs greater than 100 M {sub ⊙} are crucial for future studies of young massive star clusters at all redshifts.« less

Properties of six short-period massive binaries: A study of the effects of binarity on surface chemical abundances

NASA Astrophysics Data System (ADS)

Martins, F.; Mahy, L.; Hervé, A.

2017-11-01

Context. A significant percentage of massive stars are found in multiple systems. The effect of binarity on stellar evolution is poorly constrained. In particular, the role of tides and mass transfer on surface chemical abundances is not constrained observationally. Aims: The aim of this study is to investigate the effect of binarity on the stellar properties and surface abundances of massive binaries. Methods: We performed a spectroscopic analysis of six Galactic massive binaries. We obtained the spectra of individual components via a spectral disentangling method and subsequently analyzed these spectra by means of atmosphere models. The stellar parameters and CNO surface abundances were determined. Results: Most of these six systems are comprised of main-sequence stars. Three systems are detached, two are in contact, and no information is available for the sixth system. For 11 out of the 12 stars studied, the surface abundances are only mildly affected by stellar evolution and mixing. The surface abundances are not different from those of single stars within the uncertainties. The secondary of XZ Cep is strongly chemically enriched. Considering previous determinations of surface abundances in massive binary systems suggests that the effect of tides on chemical mixing is limited, whereas the mass transfer and removal of outer layers of the mass donor leads to the appearance of chemically processed material at the surface, although this is not systematic. The evolutionary masses of the components of our six systems are on average 16.5% higher than the dynamical masses. Some systems seem to have reached synchronization, while others may still be in a transitory phase. Based on observations made with the SOPHIE spectrograph on the 1.93 m telescope at Observatoire de Haute-Provence (OHP, CNRS/AMU), France.

Utilization of Veno-Arterial Extracorporeal Membrane Oxygenation for Massive Pulmonary Embolism.

PubMed

Pasrija, Chetan; Kronfli, Anthony; George, Praveen; Raithel, Maxwell; Boulos, Francesca; Herr, Daniel L; Gammie, James S; Pham, Si M; Griffith, Bartley P; Kon, Zachary N

2018-02-01

The management of massive pulmonary embolism remains challenging, with a considerable mortality rate. Although veno-arterial extracorporeal membrane oxygenation (VA-ECMO) for massive pulmonary embolism has been reported, its use as salvage therapy has been associated with poor outcomes. We reviewed our experience utilizing an aggressive, protocolized approach of VA-ECMO to triage, optimize, and treat these patients. All patients with a massive pulmonary embolism who were placed on VA-ECMO, as an initial intervention determined by protocol, were retrospectively reviewed. ECMO support was continued until organ optimization was achieved or neurologic status was determined. At that time, if the thrombus burden resolved, decannulation was performed. If substantial clot burden was still present with evidence of right ventricular (RV) strain, operative therapy was undertaken. Twenty patients were identified. Before cannulation, all patients had an RV-to-left ventricular ratio greater than 1.0 and severe RV dysfunction. The median duration of ECMO support was 5.1 days, with significant improvement in end-organ function. Ultimately, 40% received anticoagulation alone, 5% underwent catheter-directed therapy, and 55% underwent surgical pulmonary embolectomy. Care was withdrawn in 1 patient with a prolonged pre-cannulation cardiac arrest after confirmation of neurologic death. In-hospital and 90-day survival was 95%. At discharge, 18 of 19 patients had normal RV function, and 1 patient, who received catheter-directed therapy, had mild dysfunction. VA-ECMO appears to be an effective tool to optimize end-organ function as a bridge to recovery or intervention, with excellent outcomes. This approach may allow clinicians to better triage patients with massive pulmonary embolism to the appropriate therapy on the basis of recovery of RV function, residual thrombus burden, operative risk, and neurologic status. Copyright © 2018 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.

The definition of massive transfusion in trauma: a critical variable in examining evidence for resuscitation.

PubMed

Mitra, Biswadev; Cameron, Peter A; Gruen, Russell L; Mori, Alfredo; Fitzgerald, Mark; Street, Alison

2011-06-01

'Massive' transfusion is a poorly defined inclusion criteria for studies examining the blood and blood product that are used during trauma resuscitation. We aimed to compare the traditional definition of massive transfusion (≥10 units in 24 h) to a more acute definition of at least 5 units in 4 h. Multitrauma patients were subgrouped according to the traditional definition and compared with the acute definition. Demographics, presenting vital signs and blood results, management including transfusion practice and outcomes were retrospectively studied. Associations of transfused fresh frozen plasma:packed red blood cells (PRBC) ratios with mortality were studied. There were 927 patients who received PRBCs in the first 24 h, with 314 patients identified using the traditional definition and 303 patients using the acute definition. The patients identified using the traditional definition received 18 (12-29) units of PRBC in 24 h, significantly higher than those identified using the acute definition [15 (9-29) units, P<0.001]. The traditional definition excluded a significant proportion of patients who died in the emergency department. By using the acute definition to select a study sample, there seems to be an increase in mortality with fresh frozen plasma:PRBC ratio of 1 : 1 ratio compared with a 1 : 2 ratio. The traditional 'massive' transfusion definition not only 'dilutes' the potential study samples with a less acute group of patients, but also further excludes patients who die early. This latter group is most likely to be benefitted from any change to resuscitation practice. An acute definition of massive transfusion should be adopted when examining clinical practice during initial trauma resuscitation.

The MASSIVE Survey - X. Misalignment between Kinematic and Photometric Axes and Intrinsic Shapes of Massive Early-Type Galaxies

NASA Astrophysics Data System (ADS)

Ene, Irina; Ma, Chung-Pei; Veale, Melanie; Greene, Jenny E.; Thomas, Jens; Blakeslee, John P.; Foster, Caroline; Walsh, Jonelle L.; Ito, Jennifer; Goulding, Andy D.

2018-06-01

We use spatially resolved two-dimensional stellar velocity maps over a 107″ × 107″ field of view to investigate the kinematic features of 90 early-type galaxies above stellar mass 1011.5M⊙ in the MASSIVE survey. We measure the misalignment angle Ψ between the kinematic and photometric axes and identify local features such as velocity twists and kinematically distinct components. We find 46% of the sample to be well aligned (Ψ < 15°), 33% misaligned, and 21% without detectable rotation (non-rotators). Only 24% of the sample are fast rotators, the majority of which (91%) are aligned, whereas 57% of the slow rotators are misaligned with a nearly flat distribution of Ψ from 15° to 90°. 11 galaxies have Ψ ≳ 60° and thus exhibit minor-axis ("prolate") rotation in which the rotation is preferentially around the photometric major axis. Kinematic misalignments occur more frequently for lower galaxy spin or denser galaxy environments. Using the observed misalignment and ellipticity distributions, we infer the intrinsic shape distribution of our sample and find that MASSIVE slow rotators are consistent with being mildly triaxial, with mean axis ratios of b/a = 0.88 and c/a = 0.65. In terms of local kinematic features, 51% of the sample exhibit kinematic twists of larger than 20°, and 2 galaxies have kinematically distinct components. The frequency of misalignment and the broad distribution of Ψ reported here suggest that the most massive early-type galaxies are mildly triaxial, and that formation processes resulting in kinematically misaligned slow rotators such as gas-poor mergers occur frequently in this mass range.

Polyuria, acidosis, and coma following massive ibuprofen ingestion.

PubMed

Levine, Michael; Khurana, Amandeep; Ruha, Anne-Michelle

2010-09-01

Ibuprofen was the first over-the-counter nonsteroidal anti-inflammatory drug available in the United States. Despite being a common agent of ingestion, significant toxicity in overdose is rare. We report a case of a massive ibuprofen ingestion who developed polyuria, acidosis, and coma but survived, despite having a serum ibuprofen concentration greater than previous fatal cases. A 19-year-old man ingested 90 g (1,200 mg/kg) ibuprofen. He was initially awake and alert, but his level of consciousness deteriorated over several hours. Seven hours following the ingestion, he was intubated and mechanically ventilated secondary to loss of airway reflexes. He developed a lactic acidosis and polyuria, which lasted for nearly 24 h. His serum creatinine peaked at 1.12 mg/dL. An ibuprofen level drawn 7 h postingestion was 739.2 mg/L (therapeutic 5-49 mg/L). We describe a case of a massive ibuprofen overdose characterized by metabolic acidosis, coma, and a state of high urine output who survived with aggressive supportive care. This case is unique in several ways. First, ibuprofen levels this high have only rarely been described. Second, polyuria is very poorly described following ibuprofen ingestions.

Evidence for a massive stellar black hole in x ray Nova Muscae

NASA Technical Reports Server (NTRS)

Chen, Wan; Gehrels, Neil; Cheng, F. H.

1992-01-01

We present evidence that the X-ray Nova Muscae system contains a massive, greater than 10 M solarmass, black hole. A recently measured photometric binary mass function gives the black hole mass for this system as a function of orbital inclination angle. From the spectral redshift and width of the positron annihilation gamma-ray line observed by GRANAT/SIGMA, we find the accretion disk inclination angle to be 22 deg plus or minus 18 deg. Assuming the accretion disk lies in the orbital plane of the system, the black hole mass is found to have a lower limit of 14 M solar mass although statistics are poor. This is supported by spectral modeling of combined optical/UV/x-ray/gamma-ray data and by a new Nova Muscae distance limit we derive of greater than 3 kpc. The large mass for this black hole and the high binary mass ratio it implies (greater than 20) raise a serious challenge to theoretical models of the formation and evolution of massive binaries. The gamma-ray line technique introduced here can give tight constraints on orbital parameters when high-sensitivity line measurements are made by such missions as GRO.

Parallel integer sorting with medium and fine-scale parallelism

NASA Technical Reports Server (NTRS)

Dagum, Leonardo

1993-01-01

Two new parallel integer sorting algorithms, queue-sort and barrel-sort, are presented and analyzed in detail. These algorithms do not have optimal parallel complexity, yet they show very good performance in practice. Queue-sort designed for fine-scale parallel architectures which allow the queueing of multiple messages to the same destination. Barrel-sort is designed for medium-scale parallel architectures with a high message passing overhead. The performance results from the implementation of queue-sort on a Connection Machine CM-2 and barrel-sort on a 128 processor iPSC/860 are given. The two implementations are found to be comparable in performance but not as good as a fully vectorized bucket sort on the Cray YMP.

Sort computation

NASA Technical Reports Server (NTRS)

Dorband, John E.

1988-01-01

Sorting has long been used to organize data in preparation for further computation, but sort computation allows some types of computation to be performed during the sort. Sort aggregation and sort distribution are the two basic forms of sort computation. Sort aggregation generates an accumulative or aggregate result for each group of records and places this result in one of the records. An aggregate operation can be any operation that is both associative and commutative, i.e., any operation whose result does not depend on the order of the operands or the order in which the operations are performed. Sort distribution copies the value from a field of a specific record in a group into that field in every record of that group.

Reconstruction of a phreatic eruption on 27 September 2014 at Ontake volcano, central Japan, based on proximal pyroclastic density current and fallout deposits

NASA Astrophysics Data System (ADS)

Maeno, Fukashi; Nakada, Setsuya; Oikawa, Teruki; Yoshimoto, Mitsuhiro; Komori, Jiro; Ishizuka, Yoshihiro; Takeshita, Yoshihiro; Shimano, Taketo; Kaneko, Takayuki; Nagai, Masashi

2016-05-01

The phreatic eruption at Ontake volcano on 27 September 2014, which caused the worst volcanic disaster in the past half-century in Japan, was reconstructed based on observations of the proximal pyroclastic density current (PDC) and fallout deposits. Witness observations were also used to clarify the eruption process. The deposits are divided into three major depositional units (Units A, B, and C) which are characterized by massive, extremely poorly sorted, and multimodal grain-size distribution with 30-50 wt% of fine ash (silt-clay component). The depositional condition was initially dry but eventually changed to wet. Unit A originated from gravity-driven turbulent PDCs in the relatively dry, vent-opening phase. Unit B was then produced mainly by fallout from a vigorous moist plume during vent development. Unit C was derived from wet ash fall in the declining stage. Ballistic ejecta continuously occurred during vent opening and development. As observed in the finest population of the grain-size distribution, aggregate particles were formed throughout the eruption, and the effect of water in the plume on the aggregation increased with time and distance. Based on the deposit thickness, duration, and grain-size data, and by applying a scaling analysis using a depth-averaged model of turbulent gravity currents, the particle concentration and initial flow speed of the PDC at the summit area were estimated as 2 × 10-4-2 × 10-3 and 24-28 m/s, respectively. The tephra thinning trend in the proximal area shows a steeper slope than in similar-sized magmatic eruptions, indicating a large tephra volume deposited over a short distance owing to the wet dispersal conditions. The Ontake eruption provided an opportunity to examine the deposits from a phreatic eruption with a complex eruption sequence that reflects the effect of external water on the eruption dynamics.

Mohawk Lake or Mohawk meadow Sedimentary facies and stratigraphy of Quaternary deposits in Mohawk Valley, upper Middle Fork of the Feather River, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yount, J.C.; Harwood, D.S.; Bradbury, J.P.

1993-04-01

Mohawk Valley (MV) contain thick, well-exposed sections of Quaternary basin-fill sediments, with abundant interbedded tephra and a diverse assemblage of sedimentary facies. The eastern arm of MV, extending from Clio to Portola, contains as much as 100 m of trough cross-bedded cobble to pebble gravel and planar and trough cross-bedded coarse and medium sand, interpreted as braided stream deposits. Sections exposed in the western arm of MV consist in their lower parts of massive organic-rich silt and clay interbedded with blocky to fissile peat beds up to 1 m thick. Diatom assemblages are dominated by benthic species indicating fresh marshmore » environments with very shallow water depths of one meter or less. Proglacial lacustrine deposits of limited lateral extent are present within the outwash complexes as evidenced by varved fine sand and silt couplets, poorly sorted quartz-rich silt beds containing dropstones, and contorted beds of diamict grading laterally into slump blocks surrounded by wood-bearing silt and silty sand. The Rockland Ash (400 ka) is a prominent marker in the middle or lower part of many sections throughout MV, indicating that at least half of the basin-fill sequence is Late Quaternary in age. A log buried in diamict slumped into a proglacial lake lying approximately 3 km downstream from the Tioga Stage ice termini in Jamison and Gray Eagle Creeks yields an age of 18,715 [+-]235 C[sup 14] years BP. Previous interpretations of MV deposits originating in a large, deep lake with water depths in excess of 150 m are untenable given the sedimentary facies and diatom floras that dominate the valley. Unexhumed valleys such as Sierra Valley to the east and Long Valley to the northwest which contain large meadows traversed by braided streams are probably good analogs for the conditions that existed during the accumulation of the Mohawk Valley deposits.« less

Underestimation of boreal soil carbon stocks by mathematical soil carbon models linked to soil nutrient status

NASA Astrophysics Data System (ADS)

Ťupek, Boris; Ortiz, Carina A.; Hashimoto, Shoji; Stendahl, Johan; Dahlgren, Jonas; Karltun, Erik; Lehtonen, Aleksi

2016-08-01

Inaccurate estimate of the largest terrestrial carbon pool, soil organic carbon (SOC) stock, is the major source of uncertainty in simulating feedback of climate warming on ecosystem-atmosphere carbon dioxide exchange by process-based ecosystem and soil carbon models. Although the models need to simplify complex environmental processes of soil carbon sequestration, in a large mosaic of environments a missing key driver could lead to a modeling bias in predictions of SOC stock change.We aimed to evaluate SOC stock estimates of process-based models (Yasso07, Q, and CENTURY soil sub-model v4) against a massive Swedish forest soil inventory data set (3230 samples) organized by a recursive partitioning method into distinct soil groups with underlying SOC stock development linked to physicochemical conditions.For two-thirds of measurements all models predicted accurate SOC stock levels regardless of the detail of input data, e.g., whether they ignored or included soil properties. However, in fertile sites with high N deposition, high cation exchange capacity, or moderately increased soil water content, Yasso07 and Q models underestimated SOC stocks. In comparison to Yasso07 and Q, accounting for the site-specific soil characteristics (e. g. clay content and topsoil mineral N) by CENTURY improved SOC stock estimates for sites with high clay content, but not for sites with high N deposition.Our analysis suggested that the soils with poorly predicted SOC stocks, as characterized by the high nutrient status and well-sorted parent material, indeed have had other predominant drivers of SOC stabilization lacking in the models, presumably the mycorrhizal organic uptake and organo-mineral stabilization processes. Our results imply that the role of soil nutrient status as regulator of organic matter mineralization has to be re-evaluated, since correct SOC stocks are decisive for predicting future SOC change and soil CO2 efflux.

Small business development: who pays?

PubMed

Hellinger, D

1985-11-01

This article traces the trends in the aid community towards income generation and small business projects in developing countries since the 1950s. 1 of the great weakness of the development assistance community in the area of income generation remains the lack of analysis of population differentiation in the communities in which they work. A very different approach to the problem of the informal sector and income generation is one that responds directly to the needs of poor communities. Institutions working within poor communities and supporting multi-sectoral development while strengthening local organizations are much better situated to respond to the diverse needs of the community than are single-sector organizations with only 1 skill or service to offer. To assist significant numbers of poor people, particularly women, grouping of some sort is essential. This is not to say that group enterprise development is an easy process. The intensive and costly organizational and management assistance required to prepare a poor group for credit must be weighed along with the potential for creating a basis of long-range change within the community. To work with individual family enterprizes in a community can also have important social and economic effects, although the long-range impact may be more limited. Today, more than ever, the poor face the challenge of creating new survival mechanisms within their communities. Development agencies and assistance programs have the responsibility to help rather than hinder this process.

Derivation of sorting programs

NASA Technical Reports Server (NTRS)

Varghese, Joseph; Loganantharaj, Rasiah

1990-01-01

Program synthesis for critical applications has become a viable alternative to program verification. Nested resolution and its extension are used to synthesize a set of sorting programs from their first order logic specifications. A set of sorting programs, such as, naive sort, merge sort, and insertion sort, were successfully synthesized starting from the same set of specifications.

Spin-the-bottle Sort and Annealing Sort: Oblivious Sorting via Round-robin Random Comparisons

PubMed Central

Goodrich, Michael T.

2013-01-01

We study sorting algorithms based on randomized round-robin comparisons. Specifically, we study Spin-the-bottle sort, where comparisons are unrestricted, and Annealing sort, where comparisons are restricted to a distance bounded by a temperature parameter. Both algorithms are simple, randomized, data-oblivious sorting algorithms, which are useful in privacy-preserving computations, but, as we show, Annealing sort is much more efficient. We show that there is an input permutation that causes Spin-the-bottle sort to require Ω(n2 log n) expected time in order to succeed, and that in O(n2 log n) time this algorithm succeeds with high probability for any input. We also show there is a specification of Annealing sort that runs in O(n log n) time and succeeds with very high probability. PMID:24550575

Rejuvenation Stage Volcanics at Laeo Kilauea, Kauai, Hawaii

NASA Astrophysics Data System (ADS)

Thordarson, T.; Garcia, M.; Wanless, D.; Tagami, T.; Sano, H.

2005-12-01

The Plio-Pleistocene Koloa volcanic series represents the rejuvenated volcanism on Kauai, one of the oldest main Hawaiian Islands. The Koloa series is made up of highly alkalic basalt and associated sedimentary rocks that rest unconformably on the shield-building Waimea Canyon volcanic series. Koloa vents are dispersed across the eastern two-thirds of the island and typically consist of scoria or lava cones that fed broad lava flow fields blanketing the marginal lowlands on the south, east and north side of the island. The northernmost subaerial Koloa vents are found at Laeo Kilauea on the north shore of the island. At Laeo Kilauea the volcanic succession is unusual in that it contains the only phreatomagmatic vent structures of the Koloa series. Here an ~2-km-long costal cliff face reveals a bedded phreatomagmatic tephra sequence that is >90-m-thick and represents the remnant of an a much large tuff cone (>2-km in diameter). The tuff cone sequence is characterized by decimeter to meters thick layers, where cross-bedded ash beds alternate with massive and poorly sorted lapilli tuff beds. The cross-bedded deposits were produced by dry and wet surges, whereas the poorly sorted beds represent fall deposits produced by sustained eruption column (i.e. continuous up-rush) or tephra jets (i.e. rooster-tail explosions). The juvenile clast population of the tephra consists of olivine-phyric foidite, but it also contains abundant wall-rock lithics, including fragments of reef-limestone. The base of the tuff cone outcrops at Mokolea point on the east side of the outcrop, where phreatomagmatic tephra rests directly on an older Koloa pahoehoe flow, a olivine- and mellelite-phyric foidite lava. The tephra sequence is cut by an ~1-m-thick olivine-bearing basanite dike, which acted as a feeder for the fountain-fed spatter and lava (up to 100-m-thick) that cap the phreatomagmatic tephra sequence. These units are separated by a 2-3 m thick soil horizon formed by weathering of the tuff. These three formations have been dated by Ar-Ar giving 2.65 +/- 0.35 Ma for the age of the basal foidite lava, 1.68 +/- 0.11 Ma for the tuff cone and 0.69 +/- 0.03 Ma for the overlying fountain-fed basanite lava. Important conclusions that can be drawn from the results of this study include: (1) The characteristics of the phreatomagmatic tephra indicate that at times the tuff cone crater was filled with water implying that the eruption site was submarine and most likely located in shallow coastal waters. The presence of reef-limestone fragments in the tephra supports this notion. On the other hand, the underlying and overlying lava flows, which do extend an unknown distance beyond the current shoreline, were clearly deposited on dry land. This implies that Kauai experienced significant changes in sea level in early to mid Pleistocene times. (2) The eruptions that produced the tuff cone and the overlying fountain-fed basanite lava are one million years apart, yet the dikes that fed these eruptions appear to have followed a similar path to the surface. This indicates that the magma is utilizing preexisting structural weaknesses to reach the surface.

Safe sorting of GFP-transduced live cells for subsequent culture using a modified FACS vantage.

PubMed

Sørensen, T U; Gram, G J; Nielsen, S D; Hansen, J E

1999-12-01

A stream-in-air cell sorter enables rapid sorting to a high purity, but it is not well suited for sorting of infectious material due to the risk of airborne spread to the surroundings. A FACS Vantage cell sorter was modified for safe use with potentially HIV infected cells. Safety tests with bacteriophages were performed to evaluate the potential spread of biologically active material during cell sorting. Cells transduced with a retroviral vector carrying the gene for GFP were sorted on the basis of their GFP fluorescence, and GFP expression was followed during subsequent culture. The bacteriophage sorting showed that the biologically active material was confined to the sorting chamber. A failure mode simulating a nozzle blockage resulted in detectable droplets inside the sorting chamber, but no droplets could be detected when an additional air suction from the sorting chamber had been put on. The GFP transduced cells were sorted to 99% purity. Cells not expressing GFP at the time of sorting did not turn on the gene during subsequent culture. Un-sorted cells and cells sorted to be positive for GFP showed a decrease in the fraction of GFP positive cells during culture. Sorting of live infected cells can be performed safely and with no deleterious effects on vector expression using the modified FACS Vantage instrument. Copyright 1999 Wiley-Liss, Inc.

Conservatively treated massive prolapsed discs: a 7-year follow-up

PubMed Central

Benson, RT; Tavares, SP; Robertson, SC; Sharp, R; Marshall, RW

2010-01-01

INTRODUCTION The natural history of a lumbar hernia of the nucleus pulposus (HNP) is not fully known and clear indications for operative intervention cannot be established from the literature. Several studies have shown that the largest discs appear to have the greatest tendency to resolve. The aim of this study was to investigate whether massive prolapsed discs can be safely managed conservatively once clinical improvement has occurred. PATIENTS AND METHODS Thirty-seven patients were studied by clinical assessments and serial magnetic resonance imaging (MRI) over 2 years. Patients had severe sciatica at first, but began to show clinical improvement despite the large disc hernia-tions. Clinical assessment included the Lasegue test and neurological appraisal. The Oswestry Disability Index was used to measure function and changes in function. Serial MRI studies allowed measurement of volume changes of the herniated disc material over a period of time. RESULTS Initial follow-up at an average of 23.2 months revealed that 83% had a complete and sustained recovery at the initial follow-up. Only four patients required a discectomy. The average Oswestry disability index improved from 58% to 15%. Volumetric analysis of serial MRI scans found an average reduction of 64% in disc size. There was a poor correlation between clinical improvement and the extent of disc resolution. CONCLUSIONS A massive disc herniation can pursue a favourable clinical course. If early progress is shown, the long-term prognosis is very good and even massive disc herniations can be treated conservatively. PMID:19887021

FAK Is Required for Schwann Cell Spreading on Immature Basal Lamina to Coordinate the Radial Sorting of Peripheral Axons with Myelination

PubMed Central

Grove, Matthew

2014-01-01

Without Focal Adhesion Kinase (FAK), developing murine Schwann cells (SCs) proliferate poorly, sort axons inefficiently, and cannot myelinate peripheral nerves. Here we show that FAK is required for the development of SCs when their basal lamina (BL) is fragmentary, but not when it is mature in vivo. Mutant SCs fail to spread on fragmentary BL during development in vivo, and this is phenocopied by SCs lacking functional FAK on low laminin (LN) in vitro. Furthermore, SCs without functional FAK initiate differentiation prematurely, both in vivo and in vitro. In contrast to their behavior on high levels of LN, SCs lacking functional FAK grown on low LN display reduced spreading, proliferation, and indicators of contractility (i.e., stress fibers, arcs, and focal adhesions) and are primed to differentiate. Growth of SCs lacking functional FAK on increasing LN concentrations in vitro revealed that differentiation is not regulated by G1 arrest but rather by cell spreading and the level of contractile actomyosin. The importance of FAK as a critical regulator of the specific response of developing SCs to fragmentary BL was supported by the ability of adult FAK mutant SCs to remyelinate demyelinated adult nerves on mature BL in vivo. We conclude that FAK promotes the spreading and actomyosin contractility of immature SCs on fragmentary BL, thus maintaining their proliferation, and preventing differentiation until they reach high density, thereby promoting radial sorting. Hence, FAK has a critical role in the response of SCs to limiting BL by promoting proliferation and preventing premature SC differentiation. PMID:25274820

Speech graphs provide a quantitative measure of thought disorder in psychosis.

PubMed

Mota, Natalia B; Vasconcelos, Nivaldo A P; Lemos, Nathalia; Pieretti, Ana C; Kinouchi, Osame; Cecchi, Guillermo A; Copelli, Mauro; Ribeiro, Sidarta

2012-01-01

Psychosis has various causes, including mania and schizophrenia. Since the differential diagnosis of psychosis is exclusively based on subjective assessments of oral interviews with patients, an objective quantification of the speech disturbances that characterize mania and schizophrenia is in order. In principle, such quantification could be achieved by the analysis of speech graphs. A graph represents a network with nodes connected by edges; in speech graphs, nodes correspond to words and edges correspond to semantic and grammatical relationships. To quantify speech differences related to psychosis, interviews with schizophrenics, manics and normal subjects were recorded and represented as graphs. Manics scored significantly higher than schizophrenics in ten graph measures. Psychopathological symptoms such as logorrhea, poor speech, and flight of thoughts were grasped by the analysis even when verbosity differences were discounted. Binary classifiers based on speech graph measures sorted schizophrenics from manics with up to 93.8% of sensitivity and 93.7% of specificity. In contrast, sorting based on the scores of two standard psychiatric scales (BPRS and PANSS) reached only 62.5% of sensitivity and specificity. The results demonstrate that alterations of the thought process manifested in the speech of psychotic patients can be objectively measured using graph-theoretical tools, developed to capture specific features of the normal and dysfunctional flow of thought, such as divergence and recurrence. The quantitative analysis of speech graphs is not redundant with standard psychometric scales but rather complementary, as it yields a very accurate sorting of schizophrenics and manics. Overall, the results point to automated psychiatric diagnosis based not on what is said, but on how it is said.

Design and realization of sort manipulator of crystal-angle sort machine

NASA Astrophysics Data System (ADS)

Wang, Ming-shun; Chen, Shu-ping; Guan, Shou-ping; Zhang, Yao-wei

2005-12-01

It is a current tendency of development in automation technology to replace manpower with manipulators in working places where dangerous, harmful, heavy or repetitive work is involved. The sort manipulator is installed in a crystal-angle sort machine to take the place of manpower, and engaged in unloading and sorting work. It is the outcome of combing together mechanism, electric transmission, and pneumatic element and micro-controller control. The step motor makes the sort manipulator operate precisely. The pneumatic elements make the sort manipulator be cleverer. Micro-controller's software bestows some simple artificial intelligence on the sort manipulator, so that it can precisely repeat its unloading and sorting work. The combination of manipulator's zero position and step motor counting control puts an end to accumulating error in long time operation. A sort manipulator's design in the practice engineering has been proved to be correct and reliable.

The young stellar population of IC 1613. III. New O-type stars unveiled by GTC-OSIRIS

NASA Astrophysics Data System (ADS)

Garcia, M.; Herrero, A.

2013-03-01

Context. Very low-metallicity massive stars are key to understanding the reionization epoch. Radiation-driven winds, chief agents in the evolution of massive stars, are consequently an important ingredient in our models of the early-Universe. Recent findings hint that the winds of massive stars with poorer metallicity than the SMC may be stronger than predicted by theory. Besides calling the paradigm of radiation-driven winds into question, this result would affect the calculated ionizing radiation and mechanical feedback of massive stars, as well as the role these objects play at different stages of the Universe. Aims: The field needs a systematic study of the winds of a large sample of very metal-poor massive stars. The sampling of spectral types is particularly poor in the very early types. This paper's goal is to increase the list of known O-type stars in the dwarf irregular galaxy IC 1613, whose metallicity is lower than the SMC's roughly by a factor 2. Methods: Using the reddening-free Q pseudo-colour, evolutionary masses, and GALEX photometry, we built a list of very likely O-type stars. We obtained low-resolution (R ~ 1000) GTC-OSIRIS spectra for a fraction of them and performed spectral classification, the only way to unequivocally confirm candidate OB-stars. Results: We have discovered 8 new O-type stars in IC 1613, increasing the list of 7 known O-type stars in this galaxy by a factor of 2. The best quality spectra were analysed with the model atmosphere code FASTWIND to derive stellar parameters. We present the first spectral type - effective temperature scale for O-stars beyond the SMC. Conclusions: The target selection method is successful. From the pre-selected list of 13 OB star candidates, we have found 8 new O-stars and 4 early-B stars and provided a similar type for a formerly known early-O star. Further tests are needed, but the presented procedure can eventually make preliminary low-resolution spectroscopy to confirm candidates unnecessary. The derived effective temperature calibration for IC 1613 is about 1000 K hotter than the scale at the SMC. The analysis of an increased list of O-type stars will be crucial for studies of the winds and feedback of massive stars at all ages of the Universe. Based on observations made with the Gran Telescopio Canarias (GTC), installed in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de Canarias, on the island of La Palma. Programme ID GTC59-11B.Figures 4, 6 and Appendix A are available in electronic form at http://www.aanda.orgSpectra as FITS files are available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/551/A74

A Task Analysis of the Shift from Teacher Instructions to Self-Instructions in Performing an In-Common Task.

ERIC Educational Resources Information Center

Grote, Irene; And Others

1996-01-01

Three preschoolers performed four sorts with stimulus cards--an untaught target sort and three directly taught alternating sorts considered to self-instruct the target performance. Accuracy increased first in the skill sorts and then in the untaught target sorts. All subjects generalized to new target sorts. Correct spontaneous self-instructions…

Gravitational waves from neutron stars and asteroseismology.

PubMed

Ho, Wynn C G

2018-05-28

Neutron stars are born in the supernova explosion of massive stars. Neutron stars rotate as stably as atomic clocks and possess densities exceeding that of atomic nuclei and magnetic fields millions to billions of times stronger than those created in laboratories on the Earth. The physical properties of neutron stars are determined by many areas of fundamental physics, and detection of gravitational waves can provide invaluable insights into our understanding of these areas. Here, we describe some of the physics and astrophysics of neutron stars and how traditional electromagnetic wave observations provide clues to the sorts of gravitational waves we expect from these stars. We pay particular attention to neutron star fluid oscillations, examining their impact on electromagnetic and gravitational wave observations when these stars are in a wide binary or isolated system, then during binary inspiral right before merger, and finally at times soon after merger.This article is part of a discussion meeting issue 'The promises of gravitational-wave astronomy'. © 2018 The Author(s).

Gravitational waves from neutron stars and asteroseismology

NASA Astrophysics Data System (ADS)

Ho, Wynn C. G.

2018-05-01

Neutron stars are born in the supernova explosion of massive stars. Neutron stars rotate as stably as atomic clocks and possess densities exceeding that of atomic nuclei and magnetic fields millions to billions of times stronger than those created in laboratories on the Earth. The physical properties of neutron stars are determined by many areas of fundamental physics, and detection of gravitational waves can provide invaluable insights into our understanding of these areas. Here, we describe some of the physics and astrophysics of neutron stars and how traditional electromagnetic wave observations provide clues to the sorts of gravitational waves we expect from these stars. We pay particular attention to neutron star fluid oscillations, examining their impact on electromagnetic and gravitational wave observations when these stars are in a wide binary or isolated system, then during binary inspiral right before merger, and finally at times soon after merger. This article is part of a discussion meeting issue `The promises of gravitational-wave astronomy'.

Whole-genome analyses resolve early branches in the tree of life of modern birds

PubMed Central

Jarvis, Erich D.; Mirarab, Siavash; Aberer, Andre J.; Li, Bo; Houde, Peter; Li, Cai; Ho, Simon Y. W.; Faircloth, Brant C.; Nabholz, Benoit; Howard, Jason T.; Suh, Alexander; Weber, Claudia C.; da Fonseca, Rute R.; Li, Jianwen; Zhang, Fang; Li, Hui; Zhou, Long; Narula, Nitish; Liu, Liang; Ganapathy, Ganesh; Boussau, Bastien; Bayzid, Md. Shamsuzzoha; Zavidovych, Volodymyr; Subramanian, Sankar; Gabaldón, Toni; Capella-Gutiérrez, Salvador; Huerta-Cepas, Jaime; Rekepalli, Bhanu; Munch, Kasper; Schierup, Mikkel; Lindow, Bent; Warren, Wesley C.; Ray, David; Green, Richard E.; Bruford, Michael W.; Zhan, Xiangjiang; Dixon, Andrew; Li, Shengbin; Li, Ning; Huang, Yinhua; Derryberry, Elizabeth P.; Bertelsen, Mads Frost; Sheldon, Frederick H.; Brumfield, Robb T.; Mello, Claudio V.; Lovell, Peter V.; Wirthlin, Morgan; Schneider, Maria Paula Cruz; Prosdocimi, Francisco; Samaniego, José Alfredo; Velazquez, Amhed Missael Vargas; Alfaro-Núñez, Alonzo; Campos, Paula F.; Petersen, Bent; Sicheritz-Ponten, Thomas; Pas, An; Bailey, Tom; Scofield, Paul; Bunce, Michael; Lambert, David M.; Zhou, Qi; Perelman, Polina; Driskell, Amy C.; Shapiro, Beth; Xiong, Zijun; Zeng, Yongli; Liu, Shiping; Li, Zhenyu; Liu, Binghang; Wu, Kui; Xiao, Jin; Yinqi, Xiong; Zheng, Qiuemei; Zhang, Yong; Yang, Huanming; Wang, Jian; Smeds, Linnea; Rheindt, Frank E.; Braun, Michael; Fjeldsa, Jon; Orlando, Ludovic; Barker, F. Keith; Jønsson, Knud Andreas; Johnson, Warren; Koepfli, Klaus-Peter; O’Brien, Stephen; Haussler, David; Ryder, Oliver A.; Rahbek, Carsten; Willerslev, Eske; Graves, Gary R.; Glenn, Travis C.; McCormack, John; Burt, Dave; Ellegren, Hans; Alström, Per; Edwards, Scott V.; Stamatakis, Alexandros; Mindell, David P.; Cracraft, Joel; Braun, Edward L.; Warnow, Tandy; Jun, Wang; Gilbert, M. Thomas P.; Zhang, Guojie

2015-01-01

To better determine the history of modern birds, we performed a genome-scale phylogenetic analysis of 48 species representing all orders of Neoaves using phylogenomic methods created to handle genome-scale data. We recovered a highly resolved tree that confirms previously controversial sister or close relationships. We identified the first divergence in Neoaves, two groups we named Passerea and Columbea, representing independent lineages of diverse and convergently evolved land and water bird species. Among Passerea, we infer the common ancestor of core landbirds to have been an apex predator and confirm independent gains of vocal learning. Among Columbea, we identify pigeons and flamingoes as belonging to sister clades. Even with whole genomes, some of the earliest branches in Neoaves proved challenging to resolve, which was best explained by massive protein-coding sequence convergence and high levels of incomplete lineage sorting that occurred during a rapid radiation after the Cretaceous-Paleogene mass extinction event about 66 million years ago. PMID:25504713

Whole-genome analyses resolve early branches in the tree of life of modern birds.

PubMed

Jarvis, Erich D; Mirarab, Siavash; Aberer, Andre J; Li, Bo; Houde, Peter; Li, Cai; Ho, Simon Y W; Faircloth, Brant C; Nabholz, Benoit; Howard, Jason T; Suh, Alexander; Weber, Claudia C; da Fonseca, Rute R; Li, Jianwen; Zhang, Fang; Li, Hui; Zhou, Long; Narula, Nitish; Liu, Liang; Ganapathy, Ganesh; Boussau, Bastien; Bayzid, Md Shamsuzzoha; Zavidovych, Volodymyr; Subramanian, Sankar; Gabaldón, Toni; Capella-Gutiérrez, Salvador; Huerta-Cepas, Jaime; Rekepalli, Bhanu; Munch, Kasper; Schierup, Mikkel; Lindow, Bent; Warren, Wesley C; Ray, David; Green, Richard E; Bruford, Michael W; Zhan, Xiangjiang; Dixon, Andrew; Li, Shengbin; Li, Ning; Huang, Yinhua; Derryberry, Elizabeth P; Bertelsen, Mads Frost; Sheldon, Frederick H; Brumfield, Robb T; Mello, Claudio V; Lovell, Peter V; Wirthlin, Morgan; Schneider, Maria Paula Cruz; Prosdocimi, Francisco; Samaniego, José Alfredo; Vargas Velazquez, Amhed Missael; Alfaro-Núñez, Alonzo; Campos, Paula F; Petersen, Bent; Sicheritz-Ponten, Thomas; Pas, An; Bailey, Tom; Scofield, Paul; Bunce, Michael; Lambert, David M; Zhou, Qi; Perelman, Polina; Driskell, Amy C; Shapiro, Beth; Xiong, Zijun; Zeng, Yongli; Liu, Shiping; Li, Zhenyu; Liu, Binghang; Wu, Kui; Xiao, Jin; Yinqi, Xiong; Zheng, Qiuemei; Zhang, Yong; Yang, Huanming; Wang, Jian; Smeds, Linnea; Rheindt, Frank E; Braun, Michael; Fjeldsa, Jon; Orlando, Ludovic; Barker, F Keith; Jønsson, Knud Andreas; Johnson, Warren; Koepfli, Klaus-Peter; O'Brien, Stephen; Haussler, David; Ryder, Oliver A; Rahbek, Carsten; Willerslev, Eske; Graves, Gary R; Glenn, Travis C; McCormack, John; Burt, Dave; Ellegren, Hans; Alström, Per; Edwards, Scott V; Stamatakis, Alexandros; Mindell, David P; Cracraft, Joel; Braun, Edward L; Warnow, Tandy; Jun, Wang; Gilbert, M Thomas P; Zhang, Guojie

2014-12-12

To better determine the history of modern birds, we performed a genome-scale phylogenetic analysis of 48 species representing all orders of Neoaves using phylogenomic methods created to handle genome-scale data. We recovered a highly resolved tree that confirms previously controversial sister or close relationships. We identified the first divergence in Neoaves, two groups we named Passerea and Columbea, representing independent lineages of diverse and convergently evolved land and water bird species. Among Passerea, we infer the common ancestor of core landbirds to have been an apex predator and confirm independent gains of vocal learning. Among Columbea, we identify pigeons and flamingoes as belonging to sister clades. Even with whole genomes, some of the earliest branches in Neoaves proved challenging to resolve, which was best explained by massive protein-coding sequence convergence and high levels of incomplete lineage sorting that occurred during a rapid radiation after the Cretaceous-Paleogene mass extinction event about 66 million years ago. Copyright © 2014, American Association for the Advancement of Science.

To sort or not to sort: the impact of spike-sorting on neural decoding performance.

PubMed

Todorova, Sonia; Sadtler, Patrick; Batista, Aaron; Chase, Steven; Ventura, Valérie

2014-10-01

Brain-computer interfaces (BCIs) are a promising technology for restoring motor ability to paralyzed patients. Spiking-based BCIs have successfully been used in clinical trials to control multi-degree-of-freedom robotic devices. Current implementations of these devices require a lengthy spike-sorting step, which is an obstacle to moving this technology from the lab to the clinic. A viable alternative is to avoid spike-sorting, treating all threshold crossings of the voltage waveform on an electrode as coming from one putative neuron. It is not known, however, how much decoding information might be lost by ignoring spike identity. We present a full analysis of the effects of spike-sorting schemes on decoding performance. Specifically, we compare how well two common decoders, the optimal linear estimator and the Kalman filter, reconstruct the arm movements of non-human primates performing reaching tasks, when receiving input from various sorting schemes. The schemes we tested included: using threshold crossings without spike-sorting; expert-sorting discarding the noise; expert-sorting, including the noise as if it were another neuron; and automatic spike-sorting using waveform features. We also decoded from a joint statistical model for the waveforms and tuning curves, which does not involve an explicit spike-sorting step. Discarding the threshold crossings that cannot be assigned to neurons degrades decoding: no spikes should be discarded. Decoding based on spike-sorted units outperforms decoding based on electrodes voltage crossings: spike-sorting is useful. The four waveform based spike-sorting methods tested here yield similar decoding efficiencies: a fast and simple method is competitive. Decoding using the joint waveform and tuning model shows promise but is not consistently superior. Our results indicate that simple automated spike-sorting performs as well as the more computationally or manually intensive methods used here. Even basic spike-sorting adds value to the low-threshold waveform-crossing methods often employed in BCI decoding.

To sort or not to sort: the impact of spike-sorting on neural decoding performance

NASA Astrophysics Data System (ADS)

Todorova, Sonia; Sadtler, Patrick; Batista, Aaron; Chase, Steven; Ventura, Valérie

2014-10-01

Objective. Brain-computer interfaces (BCIs) are a promising technology for restoring motor ability to paralyzed patients. Spiking-based BCIs have successfully been used in clinical trials to control multi-degree-of-freedom robotic devices. Current implementations of these devices require a lengthy spike-sorting step, which is an obstacle to moving this technology from the lab to the clinic. A viable alternative is to avoid spike-sorting, treating all threshold crossings of the voltage waveform on an electrode as coming from one putative neuron. It is not known, however, how much decoding information might be lost by ignoring spike identity. Approach. We present a full analysis of the effects of spike-sorting schemes on decoding performance. Specifically, we compare how well two common decoders, the optimal linear estimator and the Kalman filter, reconstruct the arm movements of non-human primates performing reaching tasks, when receiving input from various sorting schemes. The schemes we tested included: using threshold crossings without spike-sorting; expert-sorting discarding the noise; expert-sorting, including the noise as if it were another neuron; and automatic spike-sorting using waveform features. We also decoded from a joint statistical model for the waveforms and tuning curves, which does not involve an explicit spike-sorting step. Main results. Discarding the threshold crossings that cannot be assigned to neurons degrades decoding: no spikes should be discarded. Decoding based on spike-sorted units outperforms decoding based on electrodes voltage crossings: spike-sorting is useful. The four waveform based spike-sorting methods tested here yield similar decoding efficiencies: a fast and simple method is competitive. Decoding using the joint waveform and tuning model shows promise but is not consistently superior. Significance. Our results indicate that simple automated spike-sorting performs as well as the more computationally or manually intensive methods used here. Even basic spike-sorting adds value to the low-threshold waveform-crossing methods often employed in BCI decoding.

Sorting nexin-2 is associated with tubular elements of the early endosome, but is not essential for retromer-mediated endosome-to-TGN transport

PubMed Central

Carlton, Jez G.; Bujny, Miriam V.; Peter, Brian J.; Oorschot, Viola M. J.; Rutherford, Anna; Arkell, Rebecca S.; Klumperman, Judith; McMahon, Harvey T.; Cullen, Peter J.

2006-01-01

Summary Sorting nexins are a large family of phox-homology-domain-containing proteins that have been implicated in the control of endosomal sorting. Sorting nexin-1 is a component of the mammalian retromer complex that regulates retrieval of the cation-independent mannose 6-phosphate receptor from endosomes to the trans-Golgi network. In yeast, retromer is composed of Vps5p (the orthologue of sorting nexin-1), Vps17p (a related sorting nexin) and a cargo selective subcomplex composed of Vps26p, Vps29p and Vps35p. With the exception of Vps17p, mammalian orthologues of all yeast retromer components have been identified. For Vps17p, one potential mammalian orthologue is sorting nexin-2. Here we show that, like sorting nexin-1, sorting nexin-2 binds phosphatidylinositol 3-monophosphate and phosphatidylinositol 3,5-bisphosphate, and possesses a Bin/Amphiphysin/Rvs domain that can sense membrane curvature. However, in contrast to sorting nexin-1, sorting nexin-2 could not induce membrane tubulation in vitro or in vivo. Functionally, we show that endogenous sorting nexin-1 and sorting nexin-2 co-localise on high curvature tubular elements of the 3-phosphoinositide-enriched early endosome, and that suppression of sorting nexin-2 does not perturb the degradative sorting of receptors for epidermal growth factor or transferrin, nor the steady-state distribution of the cation-independent mannose 6-phosphate receptor. However, suppression of sorting nexin-2 results in a subtle alteration in the kinetics of cation-independent mannose 6-phosphate receptor retrieval. These data suggest that although sorting nexin-2 may be a component of the retromer complex, its presence is not essential for the regulation of endosome-to-trans Golgi network retrieval of the cation-independent mannose 6-phosphate receptor. PMID:16179610

Development of hydronephrosis secondary to poorly managed neurogenic bowel requiring surgical disimpaction in a patient with spinal cord injury: A case report

PubMed Central

Downs, Jairon; Wolfe, Tracy; Walker, Heather

2014-01-01

Context Case of an adult patient with paraplegia managing neurogenic bladder with intermittent catheterization who was not performing a standard bowel program for management of neurogenic bowel. Findings Patient presented with increasing spasticity, fecal incontinence, and abdominal pain and ultimately was hospitalized for management. Imaging revealed massive fecal impaction, resulting in ureteral obstruction and hydronephrosis. Despite repeated aggressive bowel regimens, serial abdominal X-rays showed continued large stool burden. Ultimately surgical intervention was required to evacuate the colon and subsequently the hydronephrosis resolved. Conclusion/Clinical relevance This case illustrates the importance of proper management of neurogenic bowel, as significant medical complications, such as hydronephrosis can occur with poorly managed neurogenic bowel. PMID:24617444

Health impairments arising from drinking water polluted with domestic sewage and excreta in China.

PubMed

Ling, B

2000-01-01

Raw water of poor quality still causes many drinking-water associated health problems all over China, largely because of poor sanitation, inadequate disposal of sewage and excreta. Eutrophication due to excess of total nitrogen and phosphorous in some sources for drinking-water has led to massive proliferation of cyanobacteria. The dominant species of cyanophyta can produce microcystins, a potent liver cancer promotor. As in previous studies, high incidence of liver cancer coincided with high microcystin concentration in the source water, especially in pond water. A frequent consequence of heavy pollution of source water is further the high incidence of infectious intestinal diseases, which are more than 10-100 times as frequent in China than in developed countries.

Design of monitoring system for mail-sorting based on the Profibus S7 series PLC

NASA Astrophysics Data System (ADS)

Zhang, W.; Jia, S. H.; Wang, Y. H.; Liu, H.; Tang, G. C.

2017-01-01

With the rapid development of the postal express, the workload of mail sorting is increasing, but the automatic technology of mail sorting is not mature enough. In view of this, the system uses Siemens S7-300 PLC as the main station controller, PLC of Siemens S7-200/400 is from the station controller, through the man-machine interface configuration software MCGS, PROFIBUS-DP communication, RFID technology and mechanical sorting hand achieve mail classification sorting monitoring. Among them, distinguish mail-sorting by scanning RFID posted in the mail electronic bar code (fixed code), the system uses the corresponding controller on the acquisition of information processing, the processed information transmit to the sorting manipulator by PROFIBUS-DP. The system can realize accurate and efficient mail sorting, which will promote the development of mail sorting technology.

An Archaean submarine volcanic debris avalanche deposit, Yilgarn Craton, western Australia, with komatiite, basalt and dacite megablocks. The product of dome collapse

NASA Astrophysics Data System (ADS)

Trofimovs, J.; Cas, R. A. F.; Davis, B. K.

2004-11-01

The Boorara Domain of the Kalgoorlie Terrane, Eastern Goldfields Superterrane, western Australia contains excellent exposure of Archaean felsic and ultramafic breccias characterised by facies associations interpreted to reflect a volcanic debris avalanche mode of deposition. Such Archaean volcanic deposits are typically difficult to identify due to poor preservation and exposure. However, primary volcanological and sedimentological features are preserved within the relatively low strain and low metamorphic grade (up to lower greenschist facies) Boorara Domain that allow accurate facies reconstruction. The breccia deposit is characterised by two clast populations. A 'block facies' comprised of metre- to decimetre-scale megablocks of dacite, basalt and komatiite is preserved within a 'mixed' matrix breccia facies of angular, coarse sand- to boulder-sized clasts. The megablocks preserve original stratigraphy and show fracturing and jigsaw-fit textures within the poorly sorted, unstratified, genetically related matrix. Overlying the volcanic debris avalanche deposit, are a series of stratified horizons. These deposits show evidence of hydraulic sorting within bedforms exhibiting normal grain-size grading and tractional scour and fill structures along their basal contacts. The stratified facies is interpreted to have been deposited by high concentration, high competency turbidity currents, triggered by slope stabilization slides in the source region. Primary contacts and volcanic textures preserved in decimetre-scale volcanic blocks allow reconstruction of the pre-collapse palaeovolcanological history of the source region. The volcanic debris avalanche deposit, together with the associated stratified sedimentary horizons, were produced by sector collapse of a submarine, dacitic volcanic dome. Contemporaneous komatiite intrusion into the dacite dome may have caused dome flank instability. However, the volcanic debris avalanche trigger is interpreted to be a post-lithification tectonic influence.

Mauna Kea, Hawaii as an Analogue Site for Future Planetary Resource Exploration: Results from the 2010 ILSO-ISRU Field-Testing Campaign

NASA Technical Reports Server (NTRS)

ten Kate, I. L.; Armstrong, R.; Bernhardt, B.; Blummers, M.; Boucher, D.; Caillibot, E.; Captain, J.; Deleuterio, G.; Farmer, J. D.; Glavin, D. P.;

Dissecting Stop Transfer versus Conservative Sorting Pathways for Mitochondrial Inner Membrane Proteins in Vivo*

PubMed Central

Park, Kwangjin; Botelho, Salomé Calado; Hong, Joonki; Österberg, Marie; Kim, Hyun

2013-01-01

Mitochondrial inner membrane proteins that carry an N-terminal presequence are sorted by one of two pathways: stop transfer or conservative sorting. However, the sorting pathway is known for only a small number of proteins, in part due to the lack of robust experimental tools with which to study. Here we present an approach that facilitates determination of inner membrane protein sorting pathways in vivo by fusing a mitochondrial inner membrane protein to the C-terminal part of Mgm1p containing the rhomboid cleavage region. We validated the Mgm1 fusion approach using a set of proteins for which the sorting pathway is known, and determined sorting pathways of inner membrane proteins for which the sorting mode was previously uncharacterized. For Sdh4p, a multispanning membrane protein, our results suggest that both conservative sorting and stop transfer mechanisms are required for insertion. Furthermore, the sorting process of Mgm1 fusion proteins was analyzed under different growth conditions and yeast mutant strains that were defective in the import motor or the m-AAA protease function. Our results show that the sorting of mitochondrial proteins carrying moderately hydrophobic transmembrane segments is sensitive to cellular conditions, implying that mitochondrial import and membrane sorting in the physiological environment may be dynamically tuned. PMID:23184936

Seminal plasma affects sperm sex sorting in boars.

PubMed

Alkmin, Diego V; Parrilla, Inmaculada; Tarantini, Tatiana; Del Olmo, David; Vazquez, Juan M; Martinez, Emilio A; Roca, Jordi

2016-04-01

Two experiments were conducted in boar semen samples to evaluate how both holding time (24h) and the presence of seminal plasma (SP) before sorting affect sperm sortability and the ability of sex-sorted spermatozoa to tolerate liquid storage. Whole ejaculate samples were divided into three aliquots immediately after collection: one was diluted (1:1, v/v) in Beltsville thawing solution (BTS; 50% SP); the SP of the other two aliquots was removed and the sperm pellets were diluted with BTS + 10% of their own SP (10% SP) or BTS alone (0% SP). The three aliquots of each ejaculate were divided into two portions, one that was processed immediately for sorting and a second that was sorted after 24h storage at 15-17°C. In the first experiment, the ability to exhibit well-defined X- and Y-chromosome-bearing sperm peaks (split) in the cytometry histogram and the subsequent sorting efficiency were assessed (20 ejaculates). In contrast with holding time, the SP proportion influenced the parameters examined, as evidenced by the higher number of ejaculates exhibiting split and better sorting efficiency (P<0.05) in semen samples with 0-10% SP compared with those with 50% SP. In a second experiment, the quality (viability, total and progressive motility) and functionality (plasma membrane fluidity and intracellular generation of reactive oxygen species) of sex-sorted spermatozoa were evaluated after 0, 72 and 120h storage at 15-17°C (10 ejaculates). Holding time and SP proportion did not influence the quality or functionality of stored sex-sorted spermatozoa. In conclusion, a holding time as long as 24h before sorting did not negatively affect sex sorting efficiency or the ability of sorted boar spermatozoa to tolerate long-term liquid storage. A high proportion of SP (50%) in the semen samples before sorting reduced the number of ejaculates to be sorted and negatively influenced the sorting efficiency, but did not affect the ability of sex-sorted spermatozoa to tolerate liquid storage.

Provenance and depositional environment of epi-shelf lake sediment from Schirmacher Oasis, East Antarctica, vis-à-vis scanning electron microscopy of quartz grain, size distribution and chemical parameters

NASA Astrophysics Data System (ADS)

Shrivastava, Prakash K.; Asthana, Rajesh; Roy, Sandip K.; Swain, Ashit K.; Dharwadkar, Amit

2012-07-01

The scientific study of quartz grains is a powerful tool in deciphering the depositional environment and mode of transportation of sediments, and ultimately the origin and classification of sediments. Surface microfeatures, angularity, chemical features, and grain-size analysis of quartz grains, collectively reveal the sedimentary and physicochemical processes that acted on the grains during different stages of their geological history. Here, we apply scanning electron microscopic (SEM) analysis to evaluating the sedimentary provenance, modes of transport, weathering characteristics, alteration, and sedimentary environment of selected detrital quartz grains from the peripheral part of two epi-shelf lakes (ESL-1 and ESL-2) of the Schirmacher Oasis of East Antarctica. Our study reveals that different styles of physical weathering, erosive signatures, and chemical precipitation variably affected these quartz grains before final deposition as lake sediments. Statistical analysis (central tendencies, sorting, skewness, and kurtosis) indicates that these quartz-bearing sediments are poorly sorted glaciofluvial sediments. Saltation and suspension seem to have been the two dominant modes of transportation, and chemical analysis of these sediments indicates a gneissic provenance.

The effects of seed size on hybrids formed between oilseed rape (Brassica napus) and wild brown mustard (B. juncea).

PubMed

Liu, Yong-Bo; Tang, Zhi-Xi; Darmency, Henri; Stewart, C Neal; Di, Kun; Wei, Wei; Ma, Ke-ping

2012-01-01

Seed size has significant implications in ecology, because of its effects on plant fitness. The hybrid seeds that result from crosses between crops and their wild relatives are often small, and the consequences of this have been poorly investigated. Here we report on plant performance of hybrid and its parental transgenic oilseed rape (Brassica napus) and wild B. juncea, all grown from seeds sorted into three seed-size categories. Three seed-size categories were sorted by seed diameter for transgenic B. napus, wild B. juncea and their transgenic and non-transgenic hybrids. The seeds were sown in a field at various plant densities. Globally, small-seeded plants had delayed flowering, lower biomass, fewer flowers and seeds, and a lower thousand-seed weight. The seed-size effect varied among plant types but was not affected by plant density. There was no negative effect of seed size in hybrids, but it was correlated with reduced growth for both parents. Our results imply that the risk of further gene flow would probably not be mitigated by the small size of transgenic hybrid seeds. No fitness cost was detected to be associated with the Bt-transgene in this study.

Stochastic Model of Vesicular Sorting in Cellular Organelles

NASA Astrophysics Data System (ADS)

Vagne, Quentin; Sens, Pierre

2018-02-01

The proper sorting of membrane components by regulated exchange between cellular organelles is crucial to intracellular organization. This process relies on the budding and fusion of transport vesicles, and should be strongly influenced by stochastic fluctuations, considering the relatively small size of many organelles. We identify the perfect sorting of two membrane components initially mixed in a single compartment as a first passage process, and we show that the mean sorting time exhibits two distinct regimes as a function of the ratio of vesicle fusion to budding rates. Low ratio values lead to fast sorting but result in a broad size distribution of sorted compartments dominated by small entities. High ratio values result in two well-defined sorted compartments but sorting is exponentially slow. Our results suggest an optimal balance between vesicle budding and fusion for the rapid and efficient sorting of membrane components and highlight the importance of stochastic effects for the steady-state organization of intracellular compartments.

Identification and genetic analysis of cancer cells with PCR-activated cell sorting

PubMed Central

Eastburn, Dennis J.; Sciambi, Adam; Abate, Adam R.

2014-01-01

Cell sorting is a central tool in life science research for analyzing cellular heterogeneity or enriching rare cells out of large populations. Although methods like FACS and FISH-FC can characterize and isolate cells from heterogeneous populations, they are limited by their reliance on antibodies, or the requirement to chemically fix cells. We introduce a new cell sorting technology that robustly sorts based on sequence-specific analysis of cellular nucleic acids. Our approach, PCR-activated cell sorting (PACS), uses TaqMan PCR to detect nucleic acids within single cells and trigger their sorting. With this method, we identified and sorted prostate cancer cells from a heterogeneous population by performing >132 000 simultaneous single-cell TaqMan RT-PCR reactions targeting vimentin mRNA. Following vimentin-positive droplet sorting and downstream analysis of recovered nucleic acids, we found that cancer-specific genomes and transcripts were significantly enriched. Additionally, we demonstrate that PACS can be used to sort and enrich cells via TaqMan PCR reactions targeting single-copy genomic DNA. PACS provides a general new technical capability that expands the application space of cell sorting by enabling sorting based on cellular information not amenable to existing approaches. PMID:25030902

A patient with oeosinophilic gastroenteritis and severe malnutrition improved with home parenteral nutrition

PubMed Central

Santarpia, Lidia; Cuomo, Rosario; Camera, Luigi; Alfonsi, Lucia; Contaldo, Franco; Pasanisi, Fabrizio

2010-01-01

Oeosinophilic gastroenteritis is a chronic and rare disorder characterised by massive oeosinophilic tissue infiltration involving one or more segments of the digestive tract. The management of patients with oeosinophilic gastroenteritis is complex and the therapeutic response often poor. Here we discuss the clinical case and management of a 23-year-old man with oeosinophilic gastroenteritis since the first year of life and the decision to prescribe lifelong total parenteral nutrition. PMID:22798439

Leo P: A very low-mass, extremely metal-poor, star-forming galaxy

NASA Astrophysics Data System (ADS)

McQuinn, Kristen B.; Leo P Team

2017-01-01

Leo P is a low-luminosity dwarf galaxy just outside the Local Group with properties that make it an ideal probe of galaxy evolution at the faint-end of the luminosity function. Using combined data from 2 Hubble Space Telescope (HST) observing campaigns, the Very Large Array, the Spitzer Space telescope, as well as ground based data, we have constructed a robust evolutionary picture of Leo P. Leo P is one the most metal-poor, gas-rich galaxies ever discovered, has a stellar mass of a 5x105 Msun, comparable gas mass, and a single HII region. The star formation history reconstructed from the resolved stellar populations in Leo P shows it is unquenched, despite its very low mass. Based on the star formation history and metallicity measurements, the galaxy has lost 95% of its oxygen produced via nucleosynthesis, presumably to outflows. The neutral gas in the galaxy shows signs of rotation, although the velocity dispersion is comparable to the rotation velocity. Thus, Leo P bridges the gap between more massive dwarf irregular and less massive dwarf spheroidals on the baryonic Tully-Fisher relation. Furthermore, the galaxy hosts several, extremely dusty AGB candidates which will be probed with new HST and Spitzer observations. If confirmed as AGB stars, these may be our best local proxies for studying chemically unevolved star formation and subsequent dust production in metallicity environments comparable to the early universe.

Evaluating and Optimizing Online Advertising: Forget the Click, but There Are Good Proxies.

PubMed

Dalessandro, Brian; Hook, Rod; Perlich, Claudia; Provost, Foster

2015-06-01

Online systems promise to improve advertisement targeting via the massive and detailed data available. However, there often is too few data on exactly the outcome of interest, such as purchases, for accurate campaign evaluation and optimization (due to low conversion rates, cold start periods, lack of instrumentation of offline purchases, and long purchase cycles). This paper presents a detailed treatment of proxy modeling, which is based on the identification of a suitable alternative (proxy) target variable when data on the true objective is in short supply (or even completely nonexistent). The paper has a two-fold contribution. First, the potential of proxy modeling is demonstrated clearly, based on a massive-scale experiment across 58 real online advertising campaigns. Second, we assess the value of different specific proxies for evaluating and optimizing online display advertising, showing striking results. The results include bad news and good news. The most commonly cited and used proxy is a click on an ad. The bad news is that across a large number of campaigns, clicks are not good proxies for evaluation or for optimization: clickers do not resemble buyers. The good news is that an alternative sort of proxy performs remarkably well: observed visits to the brand's website. Specifically, predictive models built based on brand site visits-which are much more common than purchases-do a remarkably good job of predicting which browsers will make a purchase. The practical bottom line: evaluating and optimizing campaigns using clicks seems wrongheaded; however, there is an easy and attractive alternative-use a well-chosen site-visit proxy instead.

Ejection of the Massive Hydrogen-rich Envelope Timed with the Collapse of the Stripped SN 2014C

DOE Office of Scientific and Technical Information (OSTI.GOV)

Margutti, Raffaella; Kamble, A.; Milisavljevic, D.

2017-02-01

We present multi-wavelength observations of SN 2014C during the first 500 days. These observations represent the first solid detection of a young extragalactic stripped-envelope SN out to high-energy X-rays ∼40 keV. SN 2014C shows ordinary explosion parameters ( E {sub k} ∼ 1.8 × 10{sup 51} erg and M {sub ej} ∼ 1.7 M{sub ⊙}). However, over an ∼1 year timescale, SN 2014C evolved from an ordinary hydrogen-poor supernova into a strongly interacting, hydrogen-rich supernova, violating the traditional classification scheme of type-I versus type-II SNe. Signatures of the SN shock interaction with a dense medium are observed across the spectrum,more » from radio to hard X-rays, and revealed the presence of a massive shell of ∼1 M {sub ⊙} of hydrogen-rich material at ∼6 × 10{sup 16} cm. The shell was ejected by the progenitor star in the decades to centuries before collapse. This result challenges current theories of massive star evolution, as it requires a physical mechanism responsible for the ejection of the deepest hydrogen layer of H-poor SN progenitors synchronized with the onset of stellar collapse. Theoretical investigations point at binary interactions and/or instabilities during the last nuclear burning stages as potential triggers of the highly time-dependent mass loss. We constrain these scenarios utilizing the sample of 183 SNe Ib/c with public radio observations. Our analysis identifies SN 2014C-like signatures in ∼10% of SNe. This fraction is reasonably consistent with the expectation from the theory of recent envelope ejection due to binary evolution if the ejected material can survive in the close environment for 10{sup 3}–10{sup 4} years. Alternatively, nuclear burning instabilities extending to core C-burning might play a critical role.« less

Evaluation of motility, membrane status and DNA integrity of frozen-thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation.

PubMed

Montano, G A; Kraemer, D C; Love, C C; Robeck, T R; O'Brien, J K

2012-06-01

Artificial insemination (AI) with sex-sorted frozen-thawed spermatozoa has led to enhanced management of ex situ bottlenose dolphin populations. Extended distance of animals from the sorting facility can be overcome by the use of frozen-thawed, sorted and recryopreserved spermatozoa. Although one bottlenose dolphin calf had been born using sexed frozen-thawed spermatozoa derived from frozen semen, a critical evaluation of in vitro sperm quality is needed to justify the routine use of such samples in AI programs. Sperm motility parameters and plasma membrane integrity were influenced by stage of the sex-sorting process, sperm type (non-sorted and sorted) and freezing method (straw and directional) (P<0.05). After recryopreservation, sorted spermatozoa frozen with the directional freezing method maintained higher (P<0.05) motility parameters over a 24-h incubation period compared to spermatozoa frozen using straws. Quality of sperm DNA of non-sorted spermatozoa, as assessed by the sperm chromatin structure assay (SCSA), was high and remained unchanged throughout freeze-thawing and incubation processes. Though a possible interaction between Hoechst 33342 and the SCSA-derived acridine orange was observed in stained and sorted samples, the proportion of sex-sorted, recryopreserved spermatozoa exhibiting denatured DNA was low (6.6±4.1%) at 6 h after the second thawing step and remained unchanged (P>0.05) at 24 h. The viability of sorted spermatozoa was higher (P<0.05) than that of non-sorted spermatozoa across all time points after recryopreservation. Collective results indicate that bottlenose dolphin spermatozoa undergoing cryopreservation, sorting and recryopreservation are of adequate quality for use in AI.

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.

PubMed

Shields, C Wyatt; Reyes, Catherine D; López, Gabriel P

2015-03-07

Accurate and high throughput cell sorting is a critical enabling technology in molecular and cellular biology, biotechnology, and medicine. While conventional methods can provide high efficiency sorting in short timescales, advances in microfluidics have enabled the realization of miniaturized devices offering similar capabilities that exploit a variety of physical principles. We classify these technologies as either active or passive. Active systems generally use external fields (e.g., acoustic, electric, magnetic, and optical) to impose forces to displace cells for sorting, whereas passive systems use inertial forces, filters, and adhesion mechanisms to purify cell populations. Cell sorting on microchips provides numerous advantages over conventional methods by reducing the size of necessary equipment, eliminating potentially biohazardous aerosols, and simplifying the complex protocols commonly associated with cell sorting. Additionally, microchip devices are well suited for parallelization, enabling complete lab-on-a-chip devices for cellular isolation, analysis, and experimental processing. In this review, we examine the breadth of microfluidic cell sorting technologies, while focusing on those that offer the greatest potential for translation into clinical and industrial practice and that offer multiple, useful functions. We organize these sorting technologies by the type of cell preparation required (i.e., fluorescent label-based sorting, bead-based sorting, and label-free sorting) as well as by the physical principles underlying each sorting mechanism.

Microfluidic Cell Sorting: A Review of the Advances in the Separation of Cells from Debulking to Rare Cell Isolation

PubMed Central

Shields, C. Wyatt; Reyes, Catherine D.; López, Gabriel P.

2015-01-01

Accurate and high throughput cell sorting is a critical enabling technology in molecular and cellular biology, biotechnology, and medicine. While conventional methods can provide high efficiency sorting in short timescales, advances in microfluidics have enabled the realization of miniaturized devices offering similar capabilities that exploit a variety of physical principles. We classify these technologies as either active or passive. Active systems generally use external fields (e.g., acoustic, electric, magnetic, and optical) to impose forces to displace cells for sorting, whereas passive systems use inertial forces, filters, and adhesion mechanisms to purify cell populations. Cell sorting on microchips provides numerous advantages over conventional methods by reducing the size of necessary equipment, eliminating potentially biohazardous aerosols, and simplifying the complex protocols commonly associated with cell sorting. Additionally, microchip devices are well suited for parallelization, enabling complete lab-on-a-chip devices for cellular isolation, analysis, and experimental processing. In this review, we examine the breadth of microfluidic cell sorting technologies, while focusing on those that offer the greatest potential for translation into clinical and industrial practice and that offer multiple, useful functions. We organize these sorting technologies by the type of cell preparation required (i.e., fluorescent label-based sorting, bead-based sorting, and label-free sorting) as well as by the physical principles underlying each sorting mechanism. PMID:25598308

Intrusive hyaloclastite and peperitic breccias associated to sill and cryptodome emplacement on an Early Paleocene polymagmatic compound cone-dome volcanic complex from El Guanaco mine, Northern Chile

NASA Astrophysics Data System (ADS)

Páez, G. N.; Permuy Vidal, C.; Galina, M.; López, L.; Jovic, S. M.; Guido, D. M.

2018-04-01

This work explores the textural characteristics, morphology and facies architecture of well-preserved Paleocene hyaloclastic and peperitic breccias associated with subvolcanic intrusions at El Guanaco gold mine (Northern Chile). The El Guanaco mine volcanic sequence is part of a polymagmatic compound cone-dome volcanic complex grouping several dacitic domes and maar-diatremes, and subordinated subvolcanic intrusions of basaltic, andesitic and dacitic compositions. The Soledad-Peñafiel Fault System is a first order regional structure controlling the location and style of the volcanism in the region. Three different intrusive bodies (Basaltic sills, Dacitic cryptodomes and Andesitic cryptodomes) were found to intrude into a wet and poorly consolidated pyroclastic sequence representing the upper portions of a maar-diatreme. Consequently, extensive quench fragmentation and fluidization along their contacts occurred, leading to the formation of widespread breccia bodies enclosing a coherent nucleus. Differences in matrix composition allows to define two main breccias types: 1) poorly-sorted monomictic breccias (intrusive hyaloclastites) and 2) poorly-sorted tuff-matrix breccias (peperites). The observed facies architecture is interpreted as the result of the interplay of several factors, including: 1) magma viscosity, 2) the geometry of the intrusives, and 3) variations on the consolidation degree of the host rocks. Additionally, the overall geometry of each intrusive is interpreted to be controlled by the effective viscosity of the magmas along with the available magma volume at the time of the intrusions. The presence of three compositionally different subvolcanic bodies with intrusive hyaloclastite and peperite envelopes indicate, not only that all these intrusions occurred in a short period of time (probably less than 2-3 Ma), but also that the volcaniciclastic pile suffer little or none compaction nor consolidation during that time. The presence of three compositionally variated synvolcanic intrusives can be explained either by the presence of a zoned magmatic chamber feeding the volcanic complex, or more likely by the influence of the Soledad-Peñafiel Fault Zone acting as a preferential pathway for different magma compositions/sources to rise to the surface.

A Binary Array Asynchronous Sorting Algorithm with Using Petri Nets

NASA Astrophysics Data System (ADS)

Voevoda, A. A.; Romannikov, D. O.

2017-01-01

Nowadays the tasks of computations speed-up and/or their optimization are actual. Among the approaches on how to solve these tasks, a method applying approaches of parallelization and asynchronization to a sorting algorithm is considered in the paper. The sorting methods are ones of elementary methods and they are used in a huge amount of different applications. In the paper, we offer a method of an array sorting that based on a division into a set of independent adjacent pairs of numbers and their parallel and asynchronous comparison. And this one distinguishes the offered method from the traditional sorting algorithms (like quick sorting, merge sorting, insertion sorting and others). The algorithm is implemented with the use of Petri nets, like the most suitable tool for an asynchronous systems description.

Massive transfusion: an overview of the main characteristics and potential risks associated with substances used for correction of a coagulopathy.

PubMed

Seghatchian, Jerard; Samama, Meyer Michel

2012-10-01

Massive transfusion (MT) is an empiric mode of treatment advocated for uncontrolled bleeding and massive haemorrhage, aiming at optimal resuscitation and aggressive correction of coagulopathy. Conventional guidelines recommend early administration of crystalloids and colloids in conjunction with red cells, where the red cell also plays a critical haemostatic function. Plasma and platelets are only used in patients with microvascular bleeding with PT/APTT values >1.5 times the normal values and if PLT counts are below 50×10(9)/L. Massive transfusion carries a significant mortality rate (40%), which increases with the number of volume expanders and blood components transfused. Controversies still exist over the optimal ratio of blood components with respect to overall clinical outcomes and collateral damage. While inadequate transfusion is believed to be associated with poor outcomes but empirical over transfusion results in unnecessary donor exposure with an increased rate of sepsis, transfusion overload and infusion of variable amounts of some biological response modifiers (BRMs), which have the potential to cause additional harm. Alternative strategies, such as early use of tranexamic acid are helpful. However in trauma settings the use of warm fresh whole blood (WFWB) instead of reconstituted components with a different ratio of stored components might be the most cost effective and safer option to improve the patient's survival rate and minimise collateral damage. This manuscript, after a brief summary of standard medical intervention in massive transfusion focuses on the main characteristics of various substances currently available to overcome massive transfusion coagulopathy. The relative levels of some BRMs in fresh and aged blood components of the same origin are highlighted and some myths and unresolved issues related to massive transfusion practice are discussed. In brief, the coagulopathy in MT is a complex phenomenon, often complicated by chronic activation of coagulation, platelets, complement and vascular endothelial cells, where haemolysis, microvesiculation, exposure of phosphatidyl serine positive cells, altered red cells with reduced adhesive proteins and the presence of some BRM, could play a pivotal role in the coagulopathy and untoward effects. The challenges of improving the safety of massive transfusion remain as numerous and as varied as ever. The answer may reside in appropriate studies on designer whole blood, combined with new innovative tools to diagnosis a coagulopathy and an evidence based mode of therapy to establish the optimal survival benefit of patients, always taking into account the concept of harm reduction and reduction of collateral damage. Copyright © 2012 Elsevier Ltd. All rights reserved.

Wafer-scale, massively parallel carbon nanotube arrays for realizing field effect transistors with current density exceeding silicon and gallium arsenide

NASA Astrophysics Data System (ADS)

Arnold, Michael

Calculations have indicated that aligned arrays of semiconducting carbon nanotubes (CNTs) promise to outperform conventional semiconducting materials in short-channel, aggressively scaled field effect transistors (FETs) like those used in semiconductor logic and high frequency amplifier technologies. These calculations have been based on extrapolation of measurements of FETs based on one CNT, in which ballistic transport approaching the quantum conductance limit of 2Go = 4e2/h has been achieved. However, constraints in CNT sorting, processing, alignment, and contacts give rise to non-idealities when CNTs are implemented in densely-packed parallel arrays, which has resulted in a conductance per CNT far from 2Go. The consequence has been that it has been very difficult to create high performance CNT array FETs, and CNT array FETs have not outperformed but rather underperformed channel materials such as Si by 6 x or more. Here, we report nearly ballistic CNT array FETs at a density of 50 CNTs um-1, created via CNT sorting, wafer-scale alignment and assembly, and treatment. The on-state conductance in the arrays is as high as 0.46 Go per CNT, and the conductance of the arrays reaches 1.7 mS um-1, which is 7 x higher than previous state-of-the-art CNT array FETs made by other methods. The saturated on-state current density reaches 900 uA um-1 and is similar to or exceeds that of Si FETs when compared at equivalent gate oxide thickness, off-state current density, and channel length. The on-state current density exceeds that of GaAs FETs, as well. This leap in CNT FET array performance is a significant advance towards the exploitation of CNTs in high-performance semiconductor electronics technologies.

Perivascular Stem Cells: A Prospectively Purified Mesenchymal Stem Cell Population for Bone Tissue Engineering

PubMed Central

James, Aaron W.; Zara, Janette N.; Zhang, Xinli; Askarinam, Asal; Goyal, Raghav; Chiang, Michael; Yuan, Wei; Chang, Le; Corselli, Mirko; Shen, Jia; Pang, Shen; Stoker, David; Wu, Ben

2012-01-01

Adipose tissue is an ideal source of mesenchymal stem cells for bone tissue engineering: it is largely dispensable and readily accessible with minimal morbidity. However, the stromal vascular fraction (SVF) of adipose tissue is a heterogeneous cell population, which leads to unreliable bone formation. In the present study, we prospectively purified human perivascular stem cells (PSCs) from adipose tissue and compared their bone-forming capacity with that of traditionally derived SVF. PSCs are a population (sorted by fluorescence-activated cell sorting) of pericytes (CD146+CD34−CD45−) and adventitial cells (CD146−CD34+CD45−), each of which we have previously reported to have properties of mesenchymal stem cells. Here, we found that PSCs underwent osteogenic differentiation in vitro and formed bone after intramuscular implantation without the need for predifferentiation. We next sought to optimize PSCs for in vivo bone formation, adopting a demineralized bone matrix for osteoinduction and tricalcium phosphate particle formulation for protein release. Patient-matched, purified PSCs formed significantly more bone in comparison with traditionally derived SVF by all parameters. Recombinant bone morphogenetic protein 2 increased in vivo bone formation but with a massive adipogenic response. In contrast, recombinant Nel-like molecule 1 (NELL-1; a novel osteoinductive growth factor) selectively enhanced bone formation. These studies suggest that adipose-derived human PSCs are a new cell source for future efforts in skeletal regenerative medicine. Moreover, PSCs are a stem cell-based therapeutic that is readily approvable by the U.S. Food and Drug Administration, with potentially increased safety, purity, identity, potency, and efficacy. Finally, NELL-1 is a candidate growth factor able to induce human PSC osteogenesis. PMID:23197855

A Quality Sorting of Fruit Using a New Automatic Image Processing Method

NASA Astrophysics Data System (ADS)

Amenomori, Michihiro; Yokomizu, Nobuyuki

This paper presents an innovative approach for quality sorting of objects such as apples sorting in an agricultural factory, using an image processing algorithm. The objective of our approach are; firstly to sort the objects by their colors precisely; secondly to detect any irregularity of the colors surrounding the apples efficiently. An experiment has been conducted and the results have been obtained and compared with that has been preformed by human sorting process and by color sensor sorting devices. The results demonstrate that our approach is capable to sort the objects rapidly and the percentage of classification valid rate was 100 %.

Space-time philosophy reconstructed via massive Nordström scalar gravities? Laws vs. geometry, conventionality, and underdetermination

NASA Astrophysics Data System (ADS)

Pitts, J. Brian

2016-02-01

What if gravity satisfied the Klein-Gordon equation? Both particle physics from the 1920-30s and the 1890s Neumann-Seeliger modification of Newtonian gravity with exponential decay suggest considering a "graviton mass term" for gravity, which is algebraic in the potential. Unlike Nordström's "massless" theory, massive scalar gravity is strictly special relativistic in the sense of being invariant under the Poincaré group but not the 15-parameter Bateman-Cunningham conformal group. It therefore exhibits the whole of Minkowski space-time structure, albeit only indirectly concerning volumes. Massive scalar gravity is plausible in terms of relativistic field theory, while violating most interesting versions of Einstein's principles of general covariance, general relativity, equivalence, and Mach. Geometry is a poor guide to understanding massive scalar gravity(s): matter sees a conformally flat metric due to universal coupling, but gravity also sees the rest of the flat metric (barely or on long distances) in the mass term. What is the 'true' geometry, one might wonder, in line with Poincaré's modal conventionality argument? Infinitely many theories exhibit this bimetric 'geometry,' all with the total stress-energy's trace as source; thus geometry does not explain the field equations. The irrelevance of the Ehlers-Pirani-Schild construction to a critique of conventionalism becomes evident when multi-geometry theories are contemplated. Much as Seeliger envisaged, the smooth massless limit indicates underdetermination of theories by data between massless and massive scalar gravities-indeed an unconceived alternative. At least one version easily could have been developed before General Relativity; it then would have motivated thinking of Einstein's equations along the lines of Einstein's newly re-appreciated "physical strategy" and particle physics and would have suggested a rivalry from massive spin 2 variants of General Relativity (massless spin 2, Pauli and Fierz found in 1939). The Putnam-Grünbaum debate on conventionality is revisited with an emphasis on the broad modal scope of conventionalist views. Massive scalar gravity thus contributes to a historically plausible rational reconstruction of much of 20th-21st century space-time philosophy in the light of particle physics. An appendix reconsiders the Malament-Weatherall-Manchak conformal restriction of conventionality and constructs the 'universal force' influencing the causal structure. Subsequent works will discuss how massive gravity could have provided a template for a more Kant-friendly space-time theory that would have blocked Moritz Schlick's supposed refutation of synthetic a priori knowledge, and how Einstein's false analogy between the Neumann-Seeliger-Einstein modification of Newtonian gravity and the cosmological constant Λ generated lasting confusion that obscured massive gravity as a conceptual possibility.

Bladed Terrain on Pluto: Possible origins and evolution

NASA Astrophysics Data System (ADS)

Moore, Jeffrey M.; Howard, Alan D.; Umurhan, Orkan M.; White, Oliver L.; Schenk, Paul M.; Beyer, Ross A.; McKinnon, William B.; Spencer, John R.; Singer, Kelsi N.; Grundy, William M.; Earle, Alissa M.; Schmitt, Bernard; Protopapa, Silvia; Nimmo, Francis; Cruikshank, Dale P.; Hinson, David P.; Young, Leslie A.; Stern, S. Alan; Weaver, Harold A.; Olkin, Cathy B.; Ennico, Kimberly; Collins, Geoffrey; Bertrand, Tanguy; Forget, François; Scipioni, Francesca; New Horizons Science Team

2018-01-01

Bladed Terrain on Pluto consists of deposits of massive CH4, which are observed to occur within latitudes 30° of the equator and are found almost exclusively at the highest elevations (> 2 km above the mean radius). Our analysis indicates that these deposits of CH4 preferentially precipitate at low latitudes where net annual solar energy input is lowest. CH4 and N2 will both precipitate at low elevations. However, since there is much more N2 in the atmosphere than CH4, the N2 ice will dominate at these low elevations. At high elevations the atmosphere is too warm for N2 to precipitate so only CH4 can do so. We conclude that following the time of massive CH4 emplacement; there have been sufficient excursions in Pluto's climate to partially erode these deposits via sublimation into the blades we see today. Blades composed of massive CH4 ice implies that the mechanical behavior of CH4 can support at least several hundred meters of relief at Pluto surface conditions. Bladed Terrain deposits may be widespread in the low latitudes of the poorly seen sub-Charon hemisphere, based on spectral observations. If these locations are indeed Bladed Terrain deposits, they may mark heretofore unrecognized regions of high elevation.

Revealing the origin of the cold ISM in massive early-type galaxies

NASA Astrophysics Data System (ADS)

Davis, T. A.; Alatalo, K.; Bureau, M.; Young, L.; Blitz, L.; Crocker, A.; Bayet, E.; Bois, M.; Bournaud, F.; Cappellari, M.; Davies, R. L.; Duc, P.-A.; de Zeeuw, P. T.; Emsellem, E.; Falcon-Barroso, J.; Khochfar, S.; Krajnovic, D.; Kuntschner, H.; Lablanche, P.-Y.; McDermid, R. M.; Morganti, R.; Naab, T.; Sarzi, M.; Scott, N.; Serra, P.; Weijmans, A.

2013-07-01

Recently, massive early-type galaxies have shed their red-and-dead moniker, thanks to the discovery that many host residual star formation. As part of the ATLAS-3D project, we have conducted a complete, volume-limited survey of the molecular gas in 260 local early-type galaxies with the IRAM-30m telescope and the CARMA interferometer, in an attempt to understand the fuel powering this star formation. We find that around 22% of early-type galaxies in the local volume host molecular gas reservoirs. This detection rate is independent of galaxy luminosity and environment. Here we focus on how kinematic misalignment measurements and gas-to-dust ratios can be used to put constraints on the origin of the cold ISM in these systems. The origin of the cold ISM seems to depend strongly on environment, with misaligned, dust poor gas (indicative of externally acquired material) being common in the field but completely absent in rich groups and in the Virgo cluster. Very massive galaxies also appear to be devoid of accreted gas. This suggests that in the field mergers and/or cold gas accretion dominate the gas supply, while in clusters internal secular processes become more important. This implies that environment has a strong impact on the cold gas properties of ETGs.

Environmental Assessment: Explosive Ordnance Disposal Training Facility, Munitions Storage Facility, and Munitions Maintenance and Inspection Facility Westover Air Reserve Base, Massachusetts

DTIC Science & Technology

2003-08-01

laminate . The thickness of this unit ranges from 10 to more than 250 feet in the Westover ARB area. A glacial till layer of poorly sorted gravel, sand...trucks, concrete trucks, and flatbed trucks. The backhoe would be used with a percussion chisel as well as a lift bucket. Estimates were also...36.00 3.80 83.40 9.00 5.20 Concrete Truck 5 8 2 1.80 0.19 4.17 0.45 0.26 Emissions (lbs) 144.00 15.20 333.60 36.00 20.80 Flatbed Truck 10 8 1

A note on coarse-grained gravity-flow deposits within proterozoic lacustrine sedimentary rocks, Transvaal sequence, South Africa

NASA Astrophysics Data System (ADS)

Eriksson, P. G.

A widely developed, thin, coarse-matrix conglomerate occurs within early Proterozoic lacustrine mudrocks in the Transvaal Sequence, South Africa. The poorly sorted tabular chert clasts, alternation of a planar clast fabric with disorientated zones, plus normal and inverse grading in the former rock type suggest deposition by density-modified grain-flow and high density turbidity currents. The lower fan-delta slope palæenvironment inferred for the conglomerate is consistent with the lacustrine interpretation for the enclosing mudrock facies. This intracratonic setting contrasts with the marine environment generally associated with density-modified grain-flow deposits.

Neer Award 2018: Platelet-derived growth factor receptor α co-expression typifies a subset of platelet-derived growth factor receptor β-positive progenitor cells that contribute to fatty degeneration and fibrosis of the murine rotator cuff.

PubMed

Jensen, Andrew R; Kelley, Benjamin V; Mosich, Gina M; Ariniello, Allison; Eliasberg, Claire D; Vu, Brandon; Shah, Paras; Devana, Sai K; Murray, Iain R; Péault, Bruno; Dar, Ayelet; Petrigliano, Frank A

2018-04-10

After massive tears, rotator cuff muscle often undergoes atrophy, fibrosis, and fatty degeneration. These changes can lead to high surgical failure rates and poor patient outcomes. The identity of the progenitor cells involved in these processes has not been fully elucidated. Platelet-derived growth factor receptor β (PDGFRβ) and platelet-derived growth factor receptor α (PDGFRα) have previously been recognized as markers of cells involved in muscle fibroadipogenesis. We hypothesized that PDGFRα expression identifies a fibroadipogenic subset of PDGFRβ + progenitor cells that contribute to fibroadipogenesis of the rotator cuff. We created massive rotator cuff tears in a transgenic strain of mice that allows PDGFRβ + cells to be tracked via green fluorescent protein (GFP) fluorescence. We then harvested rotator cuff muscle tissues at multiple time points postoperatively and analyzed them for the presence and localization of GFP + PDGFRβ + PDGFRα + cells. We cultured, induced, and treated these cells with the molecular inhibitor CWHM-12 to assess fibrosis inhibition. GFP + PDGFRβ + PDGFRα + cells were present in rotator cuff muscle tissue and, after massive tears, localized to fibrotic and adipogenic tissues. The frequency of PDGFRβ + PDGFRα + cells increased at 5 days after massive cuff tears and decreased to basal levels within 2 weeks. PDGFRβ + PDGFRα + cells were highly adipogenic and significantly more fibrogenic than PDGFRβ + PDGFRα - cells in vitro and localized to adipogenic and fibrotic tissues in vivo. Treatment with CWHM-12 significantly decreased fibrogenesis from PDGFRβ + PDGFRα + cells. PDGFRβ + PDGFRα + cells directly contribute to fibrosis and fatty degeneration after massive rotator cuff tears in the mouse model. In addition, CWHM-12 treatment inhibits fibrogenesis from PDGFRβ + PDGFRα + cells in vitro. Clinically, perioperative PDGFRβ + PDGFRα + cell inhibition may limit rotator cuff tissue degeneration and, ultimately, improve surgical outcomes for massive rotator cuff tears. Copyright © 2018 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

Performance evaluation of firefly algorithm with variation in sorting for non-linear benchmark problems

NASA Astrophysics Data System (ADS)

Umbarkar, A. J.; Balande, U. T.; Seth, P. D.

2017-06-01

The field of nature inspired computing and optimization techniques have evolved to solve difficult optimization problems in diverse fields of engineering, science and technology. The firefly attraction process is mimicked in the algorithm for solving optimization problems. In Firefly Algorithm (FA) sorting of fireflies is done by using sorting algorithm. The original FA is proposed with bubble sort for ranking the fireflies. In this paper, the quick sort replaces bubble sort to decrease the time complexity of FA. The dataset used is unconstrained benchmark functions from CEC 2005 [22]. The comparison of FA using bubble sort and FA using quick sort is performed with respect to best, worst, mean, standard deviation, number of comparisons and execution time. The experimental result shows that FA using quick sort requires less number of comparisons but requires more execution time. The increased number of fireflies helps to converge into optimal solution whereas by varying dimension for algorithm performed better at a lower dimension than higher dimension.

Rab4b controls an early endosome sorting event by interacting with the γ-subunit of the clathrin adaptor complex 1.

PubMed

Perrin, Laura; Laura, Perrin; Lacas-Gervais, Sandra; Sandra, Lacas-Gervais; Gilleron, Jérôme; Jérôme, Gilleron; Ceppo, Franck; Franck, Ceppo; Prodon, François; François, Prodon; Benmerah, Alexandre; Alexandre, Benmerah; Tanti, Jean-François; Jean-François, Tanti; Cormont, Mireille; Mireille, Cormont

2013-11-01

The endocytic pathway is essential for cell homeostasis and numerous small Rab GTPases are involved in its control. The endocytic trafficking step controlled by Rab4b has not been elucidated, although recent data suggested it could be important for glucose homeostasis, synaptic homeostasis or adaptive immunity. Here, we show that Rab4b is required for early endosome sorting of transferrin receptors (TfRs) to the recycling endosomes, and we identified the AP1γ subunit of the clathrin adaptor AP-1 as a Rab4b effector and key component of the machinery of early endosome sorting. We show that internalised transferrin (Tf) does not reach Vamp3/Rab11 recycling endosomes in the absence of Rab4b, whereas it is rapidly recycled back to the plasma membrane. By contrast, overexpression of Rab4b leads to the accumulation of internalised Tf within AP-1- and clathrin-coated vesicles. These vesicles are poor in early and recycling endocytic markers except for TfR and require AP1γ for their formation. Furthermore, the targeted overexpression of the Rab4b-binding domain of AP1γ to early endosome upon its fusion with FYVE domains inhibited the interaction between Rab4b and endogenous AP1γ, and perturbed Tf traffic. We thus proposed that the interaction between early endocytic Rab4b and AP1γ could allow the budding of clathrin-coated vesicles for subsequent traffic to recycling endosomes. The data also uncover a novel type of endosomes, characterised by low abundance of either early or recycling endocytic markers, which could potentially be generated in cell types that naturally express high level of Rab4b.

Visualizing in situ translational activity for identifying and sorting slow-growing archaeal−bacterial consortia

PubMed Central

Hatzenpichler, Roland; Connon, Stephanie A.; Goudeau, Danielle; Malmstrom, Rex R.; Woyke, Tanja; Orphan, Victoria J.

2016-01-01

To understand the biogeochemical roles of microorganisms in the environment, it is important to determine when and under which conditions they are metabolically active. Bioorthogonal noncanonical amino acid tagging (BONCAT) can reveal active cells by tracking the incorporation of synthetic amino acids into newly synthesized proteins. The phylogenetic identity of translationally active cells can be determined by combining BONCAT with rRNA-targeted fluorescence in situ hybridization (BONCAT-FISH). In theory, BONCAT-labeled cells could be isolated with fluorescence-activated cell sorting (BONCAT-FACS) for subsequent genetic analyses. Here, in the first application, to our knowledge, of BONCAT-FISH and BONCAT-FACS within an environmental context, we probe the translational activity of microbial consortia catalyzing the anaerobic oxidation of methane (AOM), a dominant sink of methane in the ocean. These consortia, which typically are composed of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria, have been difficult to study due to their slow in situ growth rates, and fundamental questions remain about their ecology and diversity of interactions occurring between ANME and associated partners. Our activity-correlated analyses of >16,400 microbial aggregates provide the first evidence, to our knowledge, that AOM consortia affiliated with all five major ANME clades are concurrently active under controlled conditions. Surprisingly, sorting of individual BONCAT-labeled consortia followed by whole-genome amplification and 16S rRNA gene sequencing revealed previously unrecognized interactions of ANME with members of the poorly understood phylum Verrucomicrobia. This finding, together with our observation that ANME-associated Verrucomicrobia are found in a variety of geographically distinct methane seep environments, suggests a broader range of symbiotic relationships within AOM consortia than previously thought. PMID:27357680

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ethridge, F.G.; Saracino, A.M.; Burns, L.K.

The encompassing sandstones, siltstones, shales and thin conglomerates of the gasified G Coal seam at the North Knobs SDB-UCG site were deposited mainly in fluvial and poorly-drained swamp environments. These beds dip at 65/sup 0/ at the North Knobs site. Thin section and SEM analyses of the sandstones and coarse siltstones show that they are sublithic to subarkosic arenites cemented with clay minerals, calcite hematite, siderite and silica. The sandstones of Unit D directly above the coal seam have the highest concentration of calcite cement, the lowest mean grain size, and are best sorted in terms of quartz grain sizemore » variations; however, they are the worst sorted in terms of sieve size variations. Clay minerals in the sandstones are dominantly kaolinite and smectite with lesser amounts of illite and chlorite. These clays are of secondary origin. Heat alteration is present only in coals and overburden rock from cores that penetrated the cavity. Thermally altered rocks including hornfels, buchite, paralava rock and paralava breccia were found in the bottom of the dipping cavity near the injection well. The high temperature minerals of tridymite, cristobalite, mullite, cordierite, monoclinic pyroxene and high temperature plagioclase indicate that temperatures of at least 1200/sup 0/C to 1400/sup 0/C were attained in the lower part of the burn cavity. The mechanical test on the unaltered and altered overburden rock show that the most important lithologic property controlling rock strength and seismic wave velocity is the amount and type of cement in the rock. Other parameters measured were grain size, amount of clay cement, and porosity; sorting had a secondary effect on the rock strength and seismic wave velocity. There is a non-linear and direct relationship between mechanical strength and ultrasonic wave velocities for the rock tests. 30 references.« less

Single-lipid tracking on nanoscale membrane buds: The effects of curvature on lipid diffusion and sorting.

PubMed

Woodward, Xinxin; Stimpson, Eric E; Kelly, Christopher V

2018-05-29

Nanoscale membrane curvature in cells is critical for endocytosis/exocytosis and membrane trafficking. However, the biophysical ramifications of nanoscale membrane curvature on the behavior of lipids remain poorly understood. Here, we created an experimental model system of membrane curvature at a physiologically-relevant scale and obtained nanoscopic information on single-lipid distributions and dynamics. Supported lipid bilayers were created over 50 and 70 nm radius nanoparticles to create membrane buds. Single-molecule localization microscopy was performed with diverse mixtures of fluorescent and non-fluorescent lipids. Variations in lipid acyl tales length, saturation, head-group, and fluorescent labeling strategy were tested while maintaining a single fluid lipid phase throughout the membrane. Monte Carlo simulations were used to fit our experimental results and quantify the effects of curvature on the lipid diffusion and sorting. Whereas varying the composition of the non-fluorescent lipids yielded minimal changes to the curvature effects, the labeling strategy of the fluorescent lipids yielded highly varying effects of curvature. Most conditions yield single-population Brownian diffusion throughout the membrane; however, curvature-induced lipid sorting, slowing, and aggregation were observed in some conditions. Head-group labeled lipids such as DPPE-Texas Red and POPE-Rhodamine diffused >2.4× slower on the curved vs. the planar membranes; tail-labeled lipids such as NBD-PPC, TopFluor-PPC, TopFluor-PIP2, DiIC 12 , and DiIC 18 displayed no significant changes in diffusion due to the membrane curvature. This article is part of a Special Issue entitled: Emergence of Complex Behavior in Biomembranes edited by Marjorie Longo. Copyright © 2018. Published by Elsevier B.V.

Visualizing in situ translational activity for identifying and sorting slow-growing archaeal-bacterial consortia

DOE PAGES

Hatzenpichler, Roland; Connon, Stephanie A.; Goudeau, Danielle; ...

2016-06-28

To understand the biogeochemical roles of microorganisms in the environment, it is important to determine when and under which conditions they are metabolically active. Bioorthogonal noncanonical amino acid tagging (BONCAT) can reveal active cells by tracking the incorporation of synthetic amino acids into newly synthesized proteins. The phylogenetic identity of translationally active cells can be determined by combining BONCAT with rRNA-targeted fluorescence in situ hybridization (BONCAT-FISH). In theory, BONCAT-labeled cells could be isolated with fluorescence-activated cell sorting (BONCAT-FACS) for subsequent genetic analyses. Here, in the first application, to our knowledge, of BONCAT-FISH and BONCAT-FACS within an environmental context, we probemore » the translational activity of microbial consortia catalyzing the anaerobic oxidation of methane (AOM), a dominant sink of methane in the ocean. These consortia, which typically are composed of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria, have been difficult to study due to their slow in situ growth rates, and fundamental questions remain about their ecology and diversity of interactions occurring between ANME and associated partners. Our activity-correlated analyses of > 16,400 microbial aggregates provide the first evidence, to our knowledge, that AOM consortia affiliated with all five major ANME clades are concurrently active under controlled conditions. Surprisingly, sorting of individual BONCAT-labeled consortia followed by whole-genome amplification and 16S rRNA gene sequencing revealed previously unrecognized interactions of ANME with members of the poorly understood phylum Verrucomicrobia. This finding, together with our observation that ANME-associated Verrucomicrobia are found in a variety of geographically distinct methane seep environments, suggests a broader range of symbiotic relationships within AOM consortia than previously thought.« less

Differential Internalization Rates and Postendocytic Sorting of the Norepinephrine and Dopamine Transporters Are Controlled by Structural Elements in the N Termini*

PubMed Central

Vuorenpää, Anne; Jørgensen, Trine N.; Newman, Amy H.; Madsen, Kenneth L.; Scheinin, Mika

2016-01-01

The norepinephrine transporter (NET) mediates reuptake of synaptically released norepinephrine in central and peripheral noradrenergic neurons. The molecular processes governing availability of NET in the plasma membrane are poorly understood. Here we use the fluorescent cocaine analogue JHC 1-64, as well as several other approaches, to investigate the trafficking itinerary of NET in live noradrenergic neurons. Confocal imaging revealed extensive constitutive internalization of JHC 1-64-labeled NET in the neuronal somata, proximal extensions and presynaptic boutons. Phorbol 12-myristate 13-acetate increased intracellular accumulation of JHC 1-64-labeled NET and caused a parallel reduction in uptake capacity. Internalized NET strongly colocalized with the “long loop” recycling marker Rab11, whereas less overlap was seen with the “short loop” recycling marker Rab4 and the late endosomal marker Rab7. Moreover, mitigating Rab11 function by overexpression of dominant negative Rab11 impaired NET function. Sorting of NET to the Rab11 recycling compartment was further supported by confocal imaging and reversible biotinylation experiments in transfected differentiated CATH.a cells. In contrast to NET, the dopamine transporter displayed markedly less constitutive internalization and limited sorting to the Rab11 recycling compartment in the differentiated CATH.a cells. Exchange of domains between the two homologous transporters revealed that this difference was determined by non-conserved structural elements in the intracellular N terminus. We conclude that NET displays a distinct trafficking itinerary characterized by continuous shuffling between the plasma membrane and the Rab11 recycling compartment and that the functional integrity of the Rab11 compartment is critical for maintaining proper presynaptic NET function. PMID:26786096

Using concept mapping to explore why patients become lost to follow up from an antiretroviral therapy program in the Zomba District of Malawi

PubMed Central

2013-01-01

Background Retention in antiretroviral therapy (ART) programmes remains a challenge in many settings including Malawi, in part due to high numbers of losses to follow-up. Concept Mapping (CM), a mix-method participatory approach, was used to explore why patients on ART are lost to follow-up (LTFU) by identifying: 1) factors that influence patient losses to follow-up and 2) barriers to effective and efficient tracing in Zomba, Malawi. Methods CM sessions (brainstorming, sorting and rating, interpretation) were conducted in urban and rural settings in Zomba, Malawi. Participants included ART patients, ART providers, Health Surveillance Assistants, and health managers from the Zomba District Health Office. In brainstorming, participants generated statements in response to “A specific reason why an individual on ART becomes lost to follow-up is…” Participants then sorted and rated the consolidated list of brainstormed items. Analysis included inductive qualitative methods for grouping of data and quantitative cluster identification to produce visual maps which were then interpreted by participants. Results In total, 90 individuals brainstormed 371 statements, 64 consolidated statements were sorted (participant n = 46), and rated on importance and feasibility (participant n = 69). A nine-cluster concept map was generated and included both patient- and healthcare-related clusters such as: Stigma and Fears, Beliefs, Acceptance and Knowledge of ART, Access to ART, Poor Documentation, Social and Financial Support Issues, Health Worker Attitudes, Resources Needed for Effective Tracing, and Health Worker Issues Related to Tracing. Strategies to respond to the clusters were generated in Interpretation. Conclusions Multiple patient- and healthcare focused factors influence why patients become LTFU. Findings have implications particularly for programs with limited resources struggling with the retention of ART patients. PMID:23758879

Using concept mapping to explore why patients become lost to follow up from an antiretroviral therapy program in the Zomba District of Malawi.

PubMed

Rachlis, Beth; Ahmad, Farah; van Lettow, Monique; Muula, Adamson S; Semba, Medson; Cole, Donald C

2013-06-11

Retention in antiretroviral therapy (ART) programmes remains a challenge in many settings including Malawi, in part due to high numbers of losses to follow-up. Concept Mapping (CM), a mix-method participatory approach, was used to explore why patients on ART are lost to follow-up (LTFU) by identifying: 1) factors that influence patient losses to follow-up and 2) barriers to effective and efficient tracing in Zomba, Malawi. CM sessions (brainstorming, sorting and rating, interpretation) were conducted in urban and rural settings in Zomba, Malawi. Participants included ART patients, ART providers, Health Surveillance Assistants, and health managers from the Zomba District Health Office. In brainstorming, participants generated statements in response to "A specific reason why an individual on ART becomes lost to follow-up is…" Participants then sorted and rated the consolidated list of brainstormed items. Analysis included inductive qualitative methods for grouping of data and quantitative cluster identification to produce visual maps which were then interpreted by participants. In total, 90 individuals brainstormed 371 statements, 64 consolidated statements were sorted (participant n = 46), and rated on importance and feasibility (participant n = 69). A nine-cluster concept map was generated and included both patient- and healthcare-related clusters such as: Stigma and Fears, Beliefs, Acceptance and Knowledge of ART, Access to ART, Poor Documentation, Social and Financial Support Issues, Health Worker Attitudes, Resources Needed for Effective Tracing, and Health Worker Issues Related to Tracing. Strategies to respond to the clusters were generated in Interpretation. Multiple patient- and healthcare focused factors influence why patients become LTFU. Findings have implications particularly for programs with limited resources struggling with the retention of ART patients.

Independent Transport and Sorting of Functionally Distinct Protein Families in Tetrahymena thermophila Dense Core Secretory Granules▿ †

PubMed Central

Rahaman, Abdur; Miao, Wei; Turkewitz, Aaron P.

2009-01-01

Dense core granules (DCGs) in Tetrahymena thermophila contain two protein classes. Proteins in the first class, called granule lattice (Grl), coassemble to form a crystalline lattice within the granule lumen. Lattice expansion acts as a propulsive mechanism during DCG release, and Grl proteins are essential for efficient exocytosis. The second protein class, defined by a C-terminal β/γ-crystallin domain, is poorly understood. Here, we have analyzed the function and sorting of Grt1p (granule tip), which was previously identified as an abundant protein in this family. Cells lacking all copies of GRT1, together with the closely related GRT2, accumulate wild-type levels of docked DCGs. Unlike cells disrupted in any of the major GRL genes, ΔGRT1 ΔGRT2 cells show no defect in secretion, indicating that neither exocytic fusion nor core expansion depends on GRT1. These results suggest that Grl protein sorting to DCGs is independent of Grt proteins. Consistent with this, the granule core lattice in ΔGRT1 ΔGRT2 cells appears identical to that in wild-type cells by electron microscopy, and the only biochemical component visibly absent is Grt1p itself. Moreover, gel filtration showed that Grl and Grt proteins in cell homogenates exist in nonoverlapping complexes, and affinity-isolated Grt1p complexes do not contain Grl proteins. These data demonstrate that two major classes of proteins in Tetrahymena DCGs are likely to be independently transported during DCG biosynthesis and play distinct roles in granule function. The role of Grt1p may primarily be postexocytic; consistent with this idea, DCG contents from ΔGRT1 ΔGRT2 cells appear less adhesive than those from the wild type. PMID:19684282

Independent transport and sorting of functionally distinct protein families in Tetrahymena thermophila dense core secretory granules.

PubMed

Rahaman, Abdur; Miao, Wei; Turkewitz, Aaron P

2009-10-01

Dense core granules (DCGs) in Tetrahymena thermophila contain two protein classes. Proteins in the first class, called granule lattice (Grl), coassemble to form a crystalline lattice within the granule lumen. Lattice expansion acts as a propulsive mechanism during DCG release, and Grl proteins are essential for efficient exocytosis. The second protein class, defined by a C-terminal beta/gamma-crystallin domain, is poorly understood. Here, we have analyzed the function and sorting of Grt1p (granule tip), which was previously identified as an abundant protein in this family. Cells lacking all copies of GRT1, together with the closely related GRT2, accumulate wild-type levels of docked DCGs. Unlike cells disrupted in any of the major GRL genes, DeltaGRT1 DeltaGRT2 cells show no defect in secretion, indicating that neither exocytic fusion nor core expansion depends on GRT1. These results suggest that Grl protein sorting to DCGs is independent of Grt proteins. Consistent with this, the granule core lattice in DeltaGRT1 DeltaGRT2 cells appears identical to that in wild-type cells by electron microscopy, and the only biochemical component visibly absent is Grt1p itself. Moreover, gel filtration showed that Grl and Grt proteins in cell homogenates exist in nonoverlapping complexes, and affinity-isolated Grt1p complexes do not contain Grl proteins. These data demonstrate that two major classes of proteins in Tetrahymena DCGs are likely to be independently transported during DCG biosynthesis and play distinct roles in granule function. The role of Grt1p may primarily be postexocytic; consistent with this idea, DCG contents from DeltaGRT1 DeltaGRT2 cells appear less adhesive than those from the wild type.

Visualizing in situ translational activity for identifying and sorting slow-growing archaeal-bacterial consortia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hatzenpichler, Roland; Connon, Stephanie A.; Goudeau, Danielle

To understand the biogeochemical roles of microorganisms in the environment, it is important to determine when and under which conditions they are metabolically active. Bioorthogonal noncanonical amino acid tagging (BONCAT) can reveal active cells by tracking the incorporation of synthetic amino acids into newly synthesized proteins. The phylogenetic identity of translationally active cells can be determined by combining BONCAT with rRNA-targeted fluorescence in situ hybridization (BONCAT-FISH). In theory, BONCAT-labeled cells could be isolated with fluorescence-activated cell sorting (BONCAT-FACS) for subsequent genetic analyses. Here, in the first application, to our knowledge, of BONCAT-FISH and BONCAT-FACS within an environmental context, we probemore » the translational activity of microbial consortia catalyzing the anaerobic oxidation of methane (AOM), a dominant sink of methane in the ocean. These consortia, which typically are composed of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria, have been difficult to study due to their slow in situ growth rates, and fundamental questions remain about their ecology and diversity of interactions occurring between ANME and associated partners. Our activity-correlated analyses of > 16,400 microbial aggregates provide the first evidence, to our knowledge, that AOM consortia affiliated with all five major ANME clades are concurrently active under controlled conditions. Surprisingly, sorting of individual BONCAT-labeled consortia followed by whole-genome amplification and 16S rRNA gene sequencing revealed previously unrecognized interactions of ANME with members of the poorly understood phylum Verrucomicrobia. This finding, together with our observation that ANME-associated Verrucomicrobia are found in a variety of geographically distinct methane seep environments, suggests a broader range of symbiotic relationships within AOM consortia than previously thought.« less

PTF11mnb: First analog of supernova 2005bf: Long-rising, double-peaked supernova Ic from a massive progenitor*

DOE Office of Scientific and Technical Information (OSTI.GOV)

Taddia, F.; Sollerman, J.; Fremling, C.

The aim is to study PTF11mnb, a He-poor supernova (SN) whose light curves resemble those of SN 2005bf, a peculiar double-peaked stripped-envelope (SE) SN, until the declining phase after the main peak. We investigate the mechanism powering its light curve and the nature of its progenitor star. Methods. Optical photometry and spectroscopy of PTF11mnb are presented. We compared light curves, colors and spectral properties to those of SN 2005bf and normal SE SNe. We built a bolometric light curve and modeled this light curve with the SuperNova Explosion Code (SNEC) hydrodynamical code explosion of a MESA progenitor star and semi-analyticmore » models. Results. The light curve of PTF11mnb turns out to be similar to that of SN 2005bf until ~50 d when the main (secondary) peaks occur at -18.5 mag. The early peak occurs at ~20 d and is about 1.0 mag fainter. After the main peak, the decline rate of PTF11mnb is remarkably slower than what was observed in SN 2005bf, and it traces well the 56Co decay rate. The spectra of PTF11mnb reveal a SN Ic and have no traces of He unlike in the case of SN Ib 2005bf, although they have velocities comparable to those of SN 2005bf. The whole evolution of the bolometric light curve is well reproduced by the explosion of a massive (M ej = 7.8 M ⊙ ), He-poor star characterized by a double-peaked 56 Ni distribution, a total 56 Ni mass of 0.59 M ⊙ , and an explosion energy of 2.2 × 10 51 erg. Alternatively, a normal SN Ib/c explosion (M( 56Ni) = 0.11 M ⊙ , E K = 0.2 × 10 51 erg, M ej = 1 M ⊙ ) can power the first peak while a magnetar, with a magnetic field characterized by B = 5.0 × 10 14 G, and a rotation period of P = 18.1 ms, provides energy for the main peak. The early g-band light curve can be fit with a shock-breakout cooling tail or an extended envelope model from which a radius of at least 30 R ⊙ is obtained. Conclusions. We presented a scenario where PTF11mnb was the explosion of a massive, He-poor star, characterized by a double-peaked 56Ni distribution. In this case, the ejecta mass and the absence of He imply a large ZAMS mass (~85 M ⊙) for the progenitor, which most likely was a Wolf-Rayet star, surrounded by an extended envelope formed either by a pre-SN eruption or due to a binary configuration. Alternatively, PTF11mnb could be powered by a SE SN with a less massive progenitor during the first peak and by a magnetar afterward.« less

Modeling populations of rotationally mixed massive stars

NASA Astrophysics Data System (ADS)

Brott, I.

2011-02-01

Massive stars can be considered as cosmic engines. With their high luminosities, strong stellar winds and violent deaths they drive the evolution of galaxies through-out the history of the universe. Despite the importance of massive stars, their evolution is still poorly understood. Two major issues have plagued evolutionary models of massive stars until today: mixing and mass loss On the main sequence, the effects of mass loss remain limited in the considered mass and metallicity range, this thesis concentrates on the role of mixing in massive stars. This thesis approaches this problem just on the cross road between observations and simulations. The main question: Do evolutionary models of single stars, accounting for the effects of rotation, reproduce the observed properties of real stars. In particular we are interested if the evolutionary models can reproduce the surface abundance changes during the main-sequence phase. To constrain our models we build a population synthesis model for the sample of the VLT-FLAMES Survey of Massive stars, for which star-formation history and rotational velocity distribution are well constrained. We consider the four main regions of the Hunter diagram. Nitrogen un-enriched slow rotators and nitrogen enriched fast rotators that are predicted by theory. Nitrogen enriched slow rotators and nitrogen unenriched fast rotators that are not predicted by our model. We conclude that currently these comparisons are not sufficient to verify the theory of rotational mixing. Physical processes in addition to rotational mixing appear necessary to explain the stars in the later two regions. The chapters of this Thesis have been published in the following Journals: Ch. 2: ``Rotating Massive Main-Sequence Stars I: Grids of Evolutionary Models and Isochrones'', I. Brott, S. E. de Mink, M. Cantiello, N. Langer, A. de Koter, C. J. Evans, I. Hunter, C. Trundle, J.S. Vink submitted to Astronomy & Astrop hysics Ch. 3: ``The VLT-FLAMES Survey of Massive Stars: Rotation and Nitrogen Enrichment as the Key to Understanding Massive Star Evolution'', I.Hunter, I.Brott, D.J. Lennon, N. Langer, C. Trundle, A. de Koter, C.J. Evans and R.S.I. Ryans The Astrophysical Journal, 2008, 676, L29-L32 Ch. 4: ``The VLT-FLAMES Survey of Massive Stars: Constraints on Stellar Evolution from the Chemical Compositions of Rapidly Rotating Galactic and Magellanic Cloud B-type Stars '', I. Hunter, I. Brott, N. Langer, D.J. Lennon, P.L. Dufton, I.D. Howarth R.S.I. Ryan, C. Trundle, C. Evans, A. de Koter and S.J. Smartt Published in Astronomy & Astropysics, 2009, 496, 841- 853 Ch. 5: ``Rotating Massive Main-Sequence Stars II: Simulating a Population of LMC early B-type Stars as a Test of Rotational Mixing '', I. Brott, C. J. Evans, I. Hunter, A. de Koter, N. Langer, P. L. Dufton, M. Cantiello, C. Trundle, D. J. Lennon, S.E. de Mink, S.-C. Yoon, P. Anders submitted to Astronomy & Astrophysics Ch 6: ``The Nature of B Supergiants: Clues From a Steep Drop in Rotation Rates at 22 000 K - The possibility of Bi-stability braking'', Jorick S. Vink, I. Brott, G. Graefener, N. Langer, A. de Koter, D.J. Lennon Astronomy & Astrophysics, 2010, 512, L7

Learning cellular sorting pathways using protein interactions and sequence motifs.

PubMed

Lin, Tien-Ho; Bar-Joseph, Ziv; Murphy, Robert F

2011-11-01

Proper subcellular localization is critical for proteins to perform their roles in cellular functions. Proteins are transported by different cellular sorting pathways, some of which take a protein through several intermediate locations until reaching its final destination. The pathway a protein is transported through is determined by carrier proteins that bind to specific sequence motifs. In this article, we present a new method that integrates protein interaction and sequence motif data to model how proteins are sorted through these sorting pathways. We use a hidden Markov model (HMM) to represent protein sorting pathways. The model is able to determine intermediate sorting states and to assign carrier proteins and motifs to the sorting pathways. In simulation studies, we show that the method can accurately recover an underlying sorting model. Using data for yeast, we show that our model leads to accurate prediction of subcellular localization. We also show that the pathways learned by our model recover many known sorting pathways and correctly assign proteins to the path they utilize. The learned model identified new pathways and their putative carriers and motifs and these may represent novel protein sorting mechanisms. Supplementary results and software implementation are available from http://murphylab.web.cmu.edu/software/2010_RECOMB_pathways/.

Assessment and Methods for Supply-Following Loads in Modern Electricity Grids with Deep Renewables Penetration

DTIC Science & Technology

2013-12-18

from a combination of increased electricity demand, poor output from the large contingent of wind turbines in Texas (the most in the U.S.), and...2.8 GW of wind power farms in California are onshore, consist of low-altitude (m) wind turbines , and are located in 8 of California’s 58 counties...offshore wind turbines , and the improvement of turbine efficiency will enable massive potential wind resources. Looking more closely at the temporal

Birth of kids after artificial insemination with sex-sorted, frozen-thawed goat spermatozoa.

PubMed

Bathgate, R; Mace, N; Heasman, K; Evans, G; Maxwell, W M C; de Graaf, S P

2013-12-01

Successful sex-sorting of goat spermatozoa and subsequent birth of pre-sexed kids have yet to be reported. As such, a series of experiments were conducted to develop protocols for sperm-sorting (using a modified flow cytometer, MoFlo SX(®) ) and cryopreservation of goat spermatozoa. Saanen goat spermatozoa (n = 2 males) were (i) collected into Salamon's or Tris catch media post-sorting and (ii) frozen in Tris-citrate-glucose media supplemented with 5, 10 or 20% egg yolk in (iii) 0.25 ml pellets on dry ice or 0.25 ml straws in a controlled-rate freezer. Post-sort and post-thaw sperm quality were assessed by motility (CASA), viability and acrosome integrity (PI/FITC-PNA). Sex-sorted goat spermatozoa frozen in pellets displayed significantly higher post-thaw motility and viability than spermatozoa frozen in straws. Catch media and differing egg yolk concentration had no effect on the sperm parameters tested. The in vitro and in vivo fertility of sex-sorted goat spermatozoa produced with this optimum protocol were then tested by means of a heterologous ova binding assay and intrauterine artificial insemination of Saanen goat does, respectively. Sex-sorted goat spermatozoa bound to sheep ova zona pellucidae in similar numbers (p > 0.05) to non-sorted goat spermatozoa, non-sorted ram spermatozoa and sex-sorted ram spermatozoa. Following intrauterine artificial insemination with sex-sorted spermatozoa, 38% (5/13) of does kidded with 83% (3/5) of kids being of the expected sex. Does inseminated with non-sorted spermatozoa achieved a 50% (3/6) kidding rate and a sex ratio of 3 : 1 (F : M). This study demonstrates for the first time that goat spermatozoa can be sex-sorted by flow cytometry, successfully frozen and used to produce pre-sexed kids. © 2013 Blackwell Verlag GmbH.

The Masses and Stellar Content of Nuclei in Early-Type Galaxies from Multi-Band Photometry and Spectroscopy

NASA Astrophysics Data System (ADS)

Spengler, Chelsea; Côté, Patrick; Roediger, Joel; Ferrarese, Laura; Sánchez-Janssen, Rubén; Toloba, Elisa; Liu, Yiqing; Guhathakurta, Puragra; Cuillandre, Jean-Charles; Gwyn, Stephen; Zirm, Andrew; Muñoz, Roberto; Puzia, Thomas; Lançon, Ariane; Peng, Eric; Mei, Simona; Powalka, Mathieu

2018-01-01

It is now established that most, if not all, massive galaxies host central supermassive black holes (SMBHs), and that these SMBHs are linked to the growth their host galaxies as shown by several scaling relations. Within the last couple of decades, it has become apparent that most lower-mass galaxies without obvious SMBHs nevertheless contain some sort of central massive object in the form of compact stellar nuclei that also follow identical (or similar) scaling relations. These nuclei are challenging to study given their small sizes and relatively faint magnitudes, but understanding their origins and relationship to their hosts is critical to gaining a more complete picture of galaxy evolution. To that end, we highlight selected results from an analysis of 39 nuclei and their early-type hosts in the Virgo Cluster using ten broadband filters: F300W, F475W, F850LP, F160W, u*griz, and Ks. We estimate masses, metallicities and ages using simple stellar population (SSP) models. For 19 nuclei, we compare to SSP parameters derived from Keck and Gemini spectra and find reasonable agreement between the photometric and spectroscopic metallicity: the RMS scatter is 0.3 dex. We reproduce the nucleus-galaxy mass fraction of 0.33 ± 0.08% for galaxy stellar masses 108.4-1010.3 M⊙ with a typical precision of ~35% for the nuclei masses. Based on available model predictions, there is no single preferred formation scenario for nuclei, suggesting that nuclei are formed stochastically through a mix of processes. Nuclei metallicities are statistically identical to those of their hosts, appearing 0.07 ± 0.3 dex more metal-rich on average — although, omitting galaxies with unusual origins (i.e., compact ellipticals), nuclei are 0.20 ± 0.28 dex more metal-rich. We find no clear age difference between nuclei and their galaxies, with nuclei displaying a broad range of ages. Interestingly, we find that the most massive nuclei may be flatter and more closely aligned with the semi-major axes of their hosts, suggesting that they formed through predominantly dissipative processes.

Impact of the uncertainty in α-captures on {sup 22}Ne on the weak s-process in massive stars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nishimura, N.; Hirschi, R.; Pignatari, M.

2014-05-02

Massive stars at solar metallicity contribute to the production of heavy elements with atomic masses between A = 60 and A = 90 via the so-called weak s-process (which takes place during core He and shell C burning phases). Furthermore, recent studies have shown that rotation boosts the s-process production in massive stars at low metallicities, with a production that may reach the barium neutron-magic peak. These results are very sensitive to neutron source and neutron poison reaction rates. For the weak s-process, the main neutron source is the reaction {sup 22}Ne(α,n){sup 25}Mg, which is in competition with {sup 22}Ne(α,γ){supmore » 26}Mg. The uncertainty of both rates strongly affects the nucleosynthesis predictions from stellar model calculations. In this study, we investigate the impact of the uncertainty in α-captures on {sup 22}Ne on the s-process nucleosynthesis in massive stars both at solar and at very low metallicity. For this purpose, we post-process, with the Nugrid mppnp code, non-rotating and rotating evolutionary models 25M{sub ⊙} stars at two different metallicities: Z = Z{sub ⊙} and Z = 10{sup −5}Z{sub ⊙}, respectively. Our results show that uncertainty of {sup 22}Ne(α,n){sup 25}Mg and {sup 22}Ne(α,γ){sup 26}Mg rates have a significant impact on the final elemental production especially for metal poor rotating models. Beside uncertainties in the neutron source reactions, for fast rotating massive stars at low metallicity we revisit the impact of the neutron poisoning effect by the reaction chain {sup 16}O(n,γ){sup 17}O(α,γ){sup 21}Ne, in competition with the {sup 17}O(α,n){sup 20}Ne, recycling the neutrons captured by {sup 16}O.« less

Reconciling the Stellar and Nebular Spectra of High-redshift Galaxies

NASA Astrophysics Data System (ADS)

Steidel, Charles C.; Strom, Allison L.; Pettini, Max; Rudie, Gwen C.; Reddy, Naveen A.; Trainor, Ryan F.

2016-08-01

We present a combined analysis of rest-frame far-UV (FUV; 1000-2000 Å) and rest-frame optical (3600-7000 Å) composite spectra formed from very deep Keck/LRIS and Keck/MOSFIRE observations of a sample of 30 star-forming galaxies with z=2.40+/- 0.11, selected to be broadly representative of the full KBSS-MOSFIRE spectroscopic survey. Since the same massive stars are responsible for the observed FUV continuum and for the excitation of the observed nebular emission, a self-consistent stellar population synthesis model should simultaneously match the details of the FUV stellar+nebular continuum and—when inserted as the excitation source in photoionization models—predict all observed nebular emission line ratios. We find that only models including massive star binaries, having low stellar metallicity ({Z}* /{Z}⊙ ≃ 0.1) but relatively high nebular (ionized gas-phase) abundances ({Z}{{neb}}/{Z}⊙ ≃ 0.5), can successfully match all of the observational constraints. We show that this apparent discrepancy is naturally explained by highly super-solar O/Fe (≃ 4{--}5 {({{O}}/{Fe})}⊙ ), expected for a gas whose enrichment is dominated by the products of core-collapse supernovae. While O dominates the physics of the ionized gas (and thus the nebular emission lines), Fe dominates the extreme-UV (EUV) and FUV opacity and controls the mass-loss rate from massive stars, resulting in particularly dramatic effects for massive stars in binary systems. This high nebular excitation—caused by the hard EUV spectra of Fe-poor massive stars—is much more common at high redshift (z≳ 2) than low redshift due to systematic differences in the star formation history of typical galaxies. Based on data obtained at the W.M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and NASA, and was made possible by the generous financial support of the W.M. Keck Foundation.

The Evolution and Stability of Massive Stars

NASA Astrophysics Data System (ADS)

Shiode, Joshua Hajime

Massive stars are the ultimate source for nearly all the elements necessary for life. The first stars forge these elements from the sparse set of ingredients supplied by the Big Bang, and distribute enriched ashes throughout their galactic homes via their winds and explosive deaths. Subsequent generations follow suit, assembling from the enriched ashes of their predecessors. Over the last several decades, the astrophysics community has developed a sophisticated theoretical picture of the evolution of these stars, but it remains an incomplete accounting of the rich set of observations. Using state of the art models of massive stars, I have investigated the internal processes taking place throughout the life-cycles of stars spanning those from the first generation ("Population III") to the present-day ("Population I"). I will argue that early-generation stars were not highly unstable to perturbations, contrary to a host of past investigations, if a correct accounting is made for the viscous effect of convection. For later generations, those with near solar metallicity, I find that this very same convection may excite gravity-mode oscillations that produce observable brightness variations at the stellar surface when the stars are near the main sequence. If confirmed with modern high-precision monitoring experiments, like Kepler and CoRoT, the properties of observed gravity modes in massive stars could provide a direct probe of the poorly constrained physics of gravity mode excitation by convection. Finally, jumping forward in stellar evolutionary time, I propose and explore an entirely new mechanism to explain the giant eruptions observed and inferred to occur during the final phases of massive stellar evolution. This mechanism taps into the vast nuclear fusion luminosity, and accompanying convective luminosity, in the stellar core to excite waves capable of carrying a super-Eddington luminosity out to the stellar envelope. This energy transfer from the core to the envelope has the potential to unbind a significant amount of mass in close proximity to a star's eventual explosion as a core collapse supernova.

Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter.

PubMed

Schmid, Lothar; Weitz, David A; Franke, Thomas

2014-10-07

We describe a versatile microfluidic fluorescence-activated cell sorter that uses acoustic actuation to sort cells or drops at ultra-high rates. Our acoustic sorter combines the advantages of traditional fluorescence-activated cell (FACS) and droplet sorting (FADS) and is applicable for a multitude of objects. We sort aqueous droplets, at rates as high as several kHz, into two or even more outlet channels. We can also sort cells directly from the medium without prior encapsulation into drops; we demonstrate this by sorting fluorescently labeled mouse melanoma cells in a single phase fluid. Our acoustic microfluidic FACS is compatible with standard cell sorting cytometers, yet, at the same time, enables a rich variety of more sophisticated applications.

Surface acoustic wave actuated cell sorting (SAWACS).

PubMed

Franke, T; Braunmüller, S; Schmid, L; Wixforth, A; Weitz, D A

2010-03-21

We describe a novel microfluidic cell sorter which operates in continuous flow at high sorting rates. The device is based on a surface acoustic wave cell-sorting scheme and combines many advantages of fluorescence activated cell sorting (FACS) and fluorescence activated droplet sorting (FADS) in microfluidic channels. It is fully integrated on a PDMS device, and allows fast electronic control of cell diversion. We direct cells by acoustic streaming excited by a surface acoustic wave which deflects the fluid independently of the contrast in material properties of deflected objects and the continuous phase; thus the device underlying principle works without additional enhancement of the sorting by prior labelling of the cells with responsive markers such as magnetic or polarizable beads. Single cells are sorted directly from bulk media at rates as fast as several kHz without prior encapsulation into liquid droplet compartments as in traditional FACS. We have successfully directed HaCaT cells (human keratinocytes), fibroblasts from mice and MV3 melanoma cells. The low shear forces of this sorting method ensure that cells survive after sorting.

Research of grasping algorithm based on scara industrial robot

NASA Astrophysics Data System (ADS)

Peng, Tao; Zuo, Ping; Yang, Hai

2018-04-01

As the tobacco industry grows, facing the challenge of the international tobacco giant, efficient logistics service is one of the key factors. How to complete the tobacco sorting task of efficient economy is the goal of tobacco sorting and optimization research. Now the cigarette distribution system uses a single line to carry out the single brand sorting task, this article adopts a single line to realize the cigarette sorting task of different brands. Using scara robot special algorithm for sorting and packaging, the optimization scheme significantly enhances the indicators of smoke sorting system. Saving labor productivity, obviously improve production efficiency.

Learning Cellular Sorting Pathways Using Protein Interactions and Sequence Motifs

PubMed Central

Lin, Tien-Ho; Bar-Joseph, Ziv

2011-01-01

Abstract Proper subcellular localization is critical for proteins to perform their roles in cellular functions. Proteins are transported by different cellular sorting pathways, some of which take a protein through several intermediate locations until reaching its final destination. The pathway a protein is transported through is determined by carrier proteins that bind to specific sequence motifs. In this article, we present a new method that integrates protein interaction and sequence motif data to model how proteins are sorted through these sorting pathways. We use a hidden Markov model (HMM) to represent protein sorting pathways. The model is able to determine intermediate sorting states and to assign carrier proteins and motifs to the sorting pathways. In simulation studies, we show that the method can accurately recover an underlying sorting model. Using data for yeast, we show that our model leads to accurate prediction of subcellular localization. We also show that the pathways learned by our model recover many known sorting pathways and correctly assign proteins to the path they utilize. The learned model identified new pathways and their putative carriers and motifs and these may represent novel protein sorting mechanisms. Supplementary results and software implementation are available from http://murphylab.web.cmu.edu/software/2010_RECOMB_pathways/. PMID:21999284

A New Algorithm Using the Non-Dominated Tree to Improve Non-Dominated Sorting.

PubMed

Gustavsson, Patrik; Syberfeldt, Anna

2018-01-01

Non-dominated sorting is a technique often used in evolutionary algorithms to determine the quality of solutions in a population. The most common algorithm is the Fast Non-dominated Sort (FNS). This algorithm, however, has the drawback that its performance deteriorates when the population size grows. The same drawback applies also to other non-dominating sorting algorithms such as the Efficient Non-dominated Sort with Binary Strategy (ENS-BS). An algorithm suggested to overcome this drawback is the Divide-and-Conquer Non-dominated Sort (DCNS) which works well on a limited number of objectives but deteriorates when the number of objectives grows. This article presents a new, more efficient algorithm called the Efficient Non-dominated Sort with Non-Dominated Tree (ENS-NDT). ENS-NDT is an extension of the ENS-BS algorithm and uses a novel Non-Dominated Tree (NDTree) to speed up the non-dominated sorting. ENS-NDT is able to handle large population sizes and a large number of objectives more efficiently than existing algorithms for non-dominated sorting. In the article, it is shown that with ENS-NDT the runtime of multi-objective optimization algorithms such as the Non-Dominated Sorting Genetic Algorithm II (NSGA-II) can be substantially reduced.

Explaining the Ba, Y, Sr, and Eu abundance scatter in metal-poor halo stars: constraints to the r-process

NASA Astrophysics Data System (ADS)

Cescutti, G.; Chiappini, C.

2014-05-01

Context. Thanks to the heroic observational campaigns carried out in recent years we now have large samples of metal-poor stars for which measurements of detailed abundances exist. In particular, large samples of stars with metallicities -5 < [Fe/H] <-1 and measured abundances of Sr, Ba, Y, and Eu are now available. These data hold important clues on the nature of the contribution of the first stellar generations to the enrichment of our Galaxy. Aims: We aim to explain the scatter in Sr, Ba, Y, and Eu abundance ratio diagrams unveiled by the metal-poor halo stars. Methods: We computed inhomogeneous chemical evolution models for the Galactic halo assuming different scenarios for the r-process site: the electron-capture (EC) supernovae and the magnetorotationally driven (MRD) supernovae scenarios. We also considered models with and without the contribution of fast-rotating massive stars (spinstars) to an early enrichment by the s-process. A detailed comparison with the now large sample of stars with measured abundances of Sr, Ba, Y, Eu, and Fe is provided (both in terms of scatter plots and number distributions for several abundance ratios). Results: The scatter observed in these abundance ratios of the very metal-poor stars (with [Fe/H] <-2.5) can be explained by combining the s-process production in spinstars, and the r-process contribution coming from massive stars. For the r-process we have developed models for both the EC and the MRD scenarios that match the observations. Conclusions: With the present observational and theoretical constraints we cannot distinguish between the EC and the MRD scenarios in the Galactic halo. Independently of the r-process scenarios adopted, the production of elements by an s-process in spinstars is needed to reproduce the spread in abundances of the light neutron capture elements (Sr and Y) over heavy neutron capture elements (Ba and Eu). We provide a way to test our suggestions by means of the distribution of the Ba isotopic ratios in a [Ba/Fe] or [Sr/Ba] vs. [Fe/H] diagram. Appendix A is available in electronic form at http://www.aanda.org

Carbon-enhanced metal-poor stars: relics from the dark ages

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cooke, Ryan J.; Madau, Piero, E-mail: rcooke@ucolick.org

2014-08-20

We use detailed nucleosynthesis calculations and a realistic prescription for the environment of the first stars to explore the first episodes of chemical enrichment that occurred during the dark ages. Based on these calculations, we propose a novel explanation for the increased prevalence of carbon-enhanced metal-poor (CEMP) stars with decreasing Fe abundance: the observed chemistry for the most metal-poor Galactic halo stars is the result of an intimate link between the explosions of the first stars and their host minihalo's ability to retain its gas. Specifically, high-energy supernovae produce a near solar ratio of C/Fe, but are effective in evacuatingmore » the gas from their host minihalo, thereby suppressing the formation of a second generation of stars. On the other hand, minihalos that host low-energy supernovae are able to retain their gas and form a second stellar generation, but, as a result, the second stars are born with a supersolar ratio of C/Fe. Our models are able to accurately reproduce the observed distributions of [C/Fe] and [Fe/H], as well as the fraction of CEMP stars relative to non-CEMP stars as a function of [Fe/H] without any free parameters. We propose that the present lack of chemical evidence for very massive stars (≳ 140 M {sub ☉}) that ended their lives as a highly energetic pair-instability supernova does not imply that such stars were rare or did not exist; the chemical products of these very massive first stars may have been evacuated from their host minihalos and were never incorporated into subsequent generations of stars. Finally, our models suggest that the most Fe-poor stars currently known may have seen the enrichment from a small multiple of metal-free stars, and need not have been exclusively enriched by a solitary first star. These calculations also add further support to the possibility that some of the surviving dwarf satellite galaxies of the Milky Way are the relics of the first galaxies.« less

Particle Transport and Size Sorting in Bubble Microstreaming Flow

NASA Astrophysics Data System (ADS)

Thameem, Raqeeb; Rallabandi, Bhargav; Wang, Cheng; Hilgenfeldt, Sascha

2014-11-01

Ultrasonic driving of sessile semicylindrical bubbles results in powerful steady streaming flows that are robust over a wide range of driving frequencies. In a microchannel, this flow field pattern can be fine-tuned to achieve size-sensitive sorting and trapping of particles at scales much smaller than the bubble itself; the sorting mechanism has been successfully described based on simple geometrical considerations. We investigate the sorting process in more detail, both experimentally (using new parameter variations that allow greater control over the sorting) and theoretically (incorporating the device geometry as well as the superimposed channel flow into an asymptotic theory). This results in optimized criteria for size sorting and a theoretical description that closely matches the particle behavior close to the bubble, the crucial region for size sorting.

Sperm sex-sorting and preservation for managing the sex ratio and genetic diversity of the southern white rhinoceros (Ceratotherium simum simum).

PubMed

O'Brien, J K; Roth, T L; Stoops, M A; Ball, R L; Steinman, K J; Montano, G A; Love, C C; Robeck, T R

2015-01-01

White rhinoceros ejaculates (n=9) collected by electroejaculation from four males were shipped (10°C, 12h) to develop procedures for the production of chilled and frozen-thawed sex-sorted spermatozoa of adequate quality for artificial insemination (AI). Of all electroejaculate fractions, 39.7% (31/78) exhibited high quality post-collection (≥70% total motility and membrane integrity) and of those, 54.8% (17/31) presented reduced in vitro quality after transport and were retrospectively determined to exhibit urine-contamination (≥21.0μg creatinine/ml). Of fractions analyzed for creatinine concentration, 69% (44/64) were classified as urine-contaminated. For high quality non-contaminated fractions, in vitro parameters (motility, velocity, membrane, acrosome and DNA integrity) of chilled non-sorted and sorted spermatozoa were well-maintained at 5°C up to 54h post-collection, whereby >70% of post-transport (non-sorted) or post-sort (sorted) values were retained. By 54h post-collection, some motility parameters were higher (P<0.05) for non-sorted spermatozoa (total motility, rapid velocity, average path velocity) whereas all remaining motion parameters as well as membrane, acrosome and DNA integrity were similar between sperm types. In comparison with a straw method, directional freezing resulted in enhanced (P<0.05) motility and velocity of non-sorted and sorted spermatozoa, with comparable overall post-thaw quality between sperm types. High purity enrichment of X-bearing (89±6%) or Y-bearing (86±3%) spermatozoa was achieved using moderate sorting rates (2540±498X-spermatozoa/s; 1800±557Y-spermatozoa/s). Collective in vitro characteristics of sorted-chilled or sorted-frozen-thawed spermatozoa derived from high quality electroejaculates indicate acceptable fertility potential for use in AI. Copyright © 2014 Elsevier B.V. All rights reserved.

Encapsulation of sex sorted boar semen: sperm membrane status and oocyte penetration parameters.

PubMed

Spinaci, Marcella; Chlapanidas, Theodora; Bucci, Diego; Vallorani, Claudia; Perteghella, Sara; Lucconi, Giulia; Communod, Ricardo; Vigo, Daniele; Galeati, Giovanna; Faustini, Massimo; Torre, Maria Luisa

2013-03-01

Although sorted semen is experimentally used for artificial, intrauterine, and intratubal insemination and in vitro fertilization, its commercial application in swine species is still far from a reality. This is because of the low sort rate and the large number of sperm required for routine artificial insemination in the pig, compared with other production animals, and the greater susceptibility of porcine spermatozoa to stress induced by the different sex sorting steps and the postsorting handling protocols. The encapsulation technology could overcome this limitation in vivo, protecting and allowing the slow release of low-dose sorted semen. The aim of this work was to evaluate the impact of the encapsulation process on viability, acrosome integrity, and on the in vitro fertilizing potential of sorted boar semen. Our results indicate that the encapsulation technique does not damage boar sorted semen; in fact, during a 72-hour storage, no differences were observed between liquid-stored sorted semen and encapsulated sorted semen in terms of plasma membrane (39.98 ± 14.38% vs. 44.32 ± 11.72%, respectively) and acrosome integrity (74.32 ± 12.17% vs. 66.07 ± 10.83%, respectively). Encapsulated sorted spermatozoa presented a lower penetration potential than nonencapsulated ones (47.02% vs. 24.57%, respectively, P < 0.0001), and a significant reduction of polyspermic fertilization (60.76% vs. 36.43%, respectively, polyspermic ova/total ova; P < 0.0001). However, no difference (P > 0.05) was observed in terms of total efficiency of fertilization expressed as normospermic oocytes/total oocytes (18.45% vs. 15.43% for sorted diluted and sorted encapsulated semen, respectively). The encapsulation could be an alternative method of storing of pig sex sorted spermatozoa and is potentially a promising technique in order to optimize the use of low dose of sexed spermatozoa in vivo. Copyright © 2013 Elsevier Inc. All rights reserved.

Sortilin 1 Loss-of-Function Protects Against Cholestatic Liver Injury by Attenuating Hepatic Bile Acid Accumulation in Bile Duct Ligated Mice.

PubMed

Li, Jibiao; Woolbright, Benjamin L; Zhao, Wen; Wang, Yifeng; Matye, David; Hagenbuch, Bruno; Jaeschke, Hartmut; Li, Tiangang

2018-01-01

Sortilin 1 (Sort1) is an intracellular trafficking receptor that mediates protein sorting in the endocytic or secretory pathways. Recent studies revealed a role of Sort1 in the regulation of cholesterol and bile acid (BA) metabolism. This study further investigated the role of Sort1 in modulating BA detoxification and cholestatic liver injury in bile duct ligated mice. We found that Sort1 knockout (KO) mice had attenuated liver injury 24 h after bile duct ligation (BDL), which was mainly attributed to less bile infarct formation. Sham-operated Sort1 KO mice had about 20% larger BA pool size than sham-operated wildtype (WT) mice, but 24 h after BDL Sort1 KO mice had significantly attenuated hepatic BA accumulation and smaller BA pool size. After 14 days BDL, Sort1 KO mice showed significantly lower hepatic BA concentration and reduced expression of inflammatory and fibrotic marker genes, but similar degree of liver fibrosis compared with WT mice. Unbiased quantitative proteomics revealed that Sort1 KO mice had increased hepatic BA sulfotransferase 2A1, but unaltered phase-I BA metabolizing cytochrome P450s or phase-III BA efflux transporters. Consistently, Sort1 KO mice showed elevated plasma sulfated taurocholate after BDL. Finally, we found that liver Sort1 was repressed after BDL, which may be due to BA activation of farnesoid x receptor. In conclusion, we report a role of Sort1 in the regulation of hepatic BA detoxification and cholestatic liver injury in mice. The mechanisms underlying increased hepatic BA elimination in Sort1 KO mice after BDL require further investigation. © The Author 2017. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Reducing 4D CT artifacts using optimized sorting based on anatomic similarity.

PubMed

Johnston, Eric; Diehn, Maximilian; Murphy, James D; Loo, Billy W; Maxim, Peter G

2011-05-01

Four-dimensional (4D) computed tomography (CT) has been widely used as a tool to characterize respiratory motion in radiotherapy. The two most commonly used 4D CT algorithms sort images by the associated respiratory phase or displacement into a predefined number of bins, and are prone to image artifacts at transitions between bed positions. The purpose of this work is to demonstrate a method of reducing motion artifacts in 4D CT by incorporating anatomic similarity into phase or displacement based sorting protocols. Ten patient datasets were retrospectively sorted using both the displacement and phase based sorting algorithms. Conventional sorting methods allow selection of only the nearest-neighbor image in time or displacement within each bin. In our method, for each bed position either the displacement or the phase defines the center of a bin range about which several candidate images are selected. The two dimensional correlation coefficients between slices bordering the interface between adjacent couch positions are then calculated for all candidate pairings. Two slices have a high correlation if they are anatomically similar. Candidates from each bin are then selected to maximize the slice correlation over the entire data set using the Dijkstra's shortest path algorithm. To assess the reduction of artifacts, two thoracic radiation oncologists independently compared the resorted 4D datasets pairwise with conventionally sorted datasets, blinded to the sorting method, to choose which had the least motion artifacts. Agreement between reviewers was evaluated using the weighted kappa score. Anatomically based image selection resulted in 4D CT datasets with significantly reduced motion artifacts with both displacement (P = 0.0063) and phase sorting (P = 0.00022). There was good agreement between the two reviewers, with complete agreement 34 times and complete disagreement 6 times. Optimized sorting using anatomic similarity significantly reduces 4D CT motion artifacts compared to conventional phase or displacement based sorting. This improved sorting algorithm is a straightforward extension of the two most common 4D CT sorting algorithms.

NIH Toolbox Cognition Battery (NIHTB-CB): list sorting test to measure working memory.

PubMed

Tulsky, David S; Carlozzi, Noelle; Chiaravalloti, Nancy D; Beaumont, Jennifer L; Kisala, Pamela A; Mungas, Dan; Conway, Kevin; Gershon, Richard

2014-07-01

The List Sorting Working Memory Test was designed to assess working memory (WM) as part of the NIH Toolbox Cognition Battery. List Sorting is a sequencing task requiring children and adults to sort and sequence stimuli that are presented visually and auditorily. Validation data are presented for 268 participants ages 20 to 85 years. A subset of participants (N=89) was retested 7 to 21 days later. As expected, the List Sorting Test had moderately high correlations with other measures of working memory and executive functioning (convergent validity) but a low correlation with a test of receptive vocabulary (discriminant validity). Furthermore, List Sorting demonstrates expected changes over the age span and has excellent test-retest reliability. Collectively, these results provide initial support for the construct validity of the List Sorting Working Memory Measure as a measure of working memory. However, the relationship between the List Sorting Test and general executive function has yet to be determined.

Manual sorting to eliminate aflatoxin from peanuts.

PubMed

Galvez, F C F; Francisco, M L D L; Villarino, B J; Lustre, A O; Resurreccion, A V A

2003-10-01

A manual sorting procedure was developed to eliminate aflatoxin contamination from peanuts. The efficiency of the sorting process in eliminating aflatoxin-contaminated kernels from lots of raw peanuts was verified. The blanching of 20 kg of peanuts at 140 degrees C for 25 min in preheated roasters facilitated the manual sorting of aflatoxin-contaminated kernels after deskinning. The manual sorting of raw materials with initially high aflatoxin contents (300 ppb) resulted in aflatoxin-free peanuts (i.e., peanuts in which no aflatoxin was detected). Verification procedures showed that the sorted sound peanuts contained no aflatoxin or contained low levels (<15 ppb) of aflatoxin. The results obtained confirmed that the sorting process was effective in separating contaminated peanuts whether or nor contamination was extensive. At the commercial level, when roasters were not preheated, the dry blanching of 50 kg of peanuts for 45 to 55 min facilitated the proper deskinning and subsequent manual sorting of aflatoxin-contaminated peanut kernels from sound kernels.

Automated spike sorting algorithm based on Laplacian eigenmaps and k-means clustering.

PubMed

Chah, E; Hok, V; Della-Chiesa, A; Miller, J J H; O'Mara, S M; Reilly, R B

2011-02-01

This study presents a new automatic spike sorting method based on feature extraction by Laplacian eigenmaps combined with k-means clustering. The performance of the proposed method was compared against previously reported algorithms such as principal component analysis (PCA) and amplitude-based feature extraction. Two types of classifier (namely k-means and classification expectation-maximization) were incorporated within the spike sorting algorithms, in order to find a suitable classifier for the feature sets. Simulated data sets and in-vivo tetrode multichannel recordings were employed to assess the performance of the spike sorting algorithms. The results show that the proposed algorithm yields significantly improved performance with mean sorting accuracy of 73% and sorting error of 10% compared to PCA which combined with k-means had a sorting accuracy of 58% and sorting error of 10%.A correction was made to this article on 22 February 2011. The spacing of the title was amended on the abstract page. No changes were made to the article PDF and the print version was unaffected.

A Simple Deep Learning Method for Neuronal Spike Sorting

NASA Astrophysics Data System (ADS)

Yang, Kai; Wu, Haifeng; Zeng, Yu

2017-10-01

Spike sorting is one of key technique to understand brain activity. With the development of modern electrophysiology technology, some recent multi-electrode technologies have been able to record the activity of thousands of neuronal spikes simultaneously. The spike sorting in this case will increase the computational complexity of conventional sorting algorithms. In this paper, we will focus spike sorting on how to reduce the complexity, and introduce a deep learning algorithm, principal component analysis network (PCANet) to spike sorting. The introduced method starts from a conventional model and establish a Toeplitz matrix. Through the column vectors in the matrix, we trains a PCANet, where some eigenvalue vectors of spikes could be extracted. Finally, support vector machine (SVM) is used to sort spikes. In experiments, we choose two groups of simulated data from public databases availably and compare this introduced method with conventional methods. The results indicate that the introduced method indeed has lower complexity with the same sorting errors as the conventional methods.

NIH Toolbox Cognition Battery (NIHTB-CB): The List Sorting Test to Measure Working Memory

PubMed Central

Tulsky, David S.; Carlozzi, Noelle; Chiaravalloti, Nancy D.; Beaumont, Jennifer L.; Kisala, Pamela A.; Mungas, Dan; Conway, Kevin; Gershon, Richard

2015-01-01

The List Sorting Working Memory Test was designed to assess working memory (WM) as part of the NIH Toolbox Cognition Battery. List Sorting is a sequencing task requiring children and adults to sort and sequence stimuli that are presented visually and auditorily. Validation data are presented for 268 participants ages 20 to 85 years. A subset of participants (N=89) was retested 7 to 21 days later. As expected, the List Sorting Test had moderately high correlations with other measures of working memory and executive functioning (convergent validity) but a low correlation with a test of receptive vocabulary (discriminant validity). Furthermore, List Sorting demonstrates expected changes over the age span and has excellent test-retest reliability. Collectively, these results provide initial support the construct validity of the List Sorting Working Memory Measure as a measure of working memory. However, the relation between the List Sorting Test and general executive function has yet to be determined. PMID:24959983

Seafloor massive sulfide deposits support unique megafaunal assemblages: Implications for seabed mining and conservation.

PubMed

Boschen, Rachel E; Rowden, Ashley A; Clark, Malcolm R; Pallentin, Arne; Gardner, Jonathan P A

2016-04-01

Mining of seafloor massive sulfides (SMS) is imminent, but the ecology of assemblages at SMS deposits is poorly known. Proposed conservation strategies include protected areas to preserve biodiversity at risk from mining impacts. Determining site suitability requires biological characterisation of the mine site and protected area(s). Video survey of a proposed mine site and protected area off New Zealand revealed unique megafaunal assemblages at the mine site. Significant relationships were identified between assemblage structure and environmental conditions, including hydrothermal features. Unique assemblages occurred at both active and inactive chimneys and are particularly at risk from mining-related impacts. The occurrence of unique assemblages at the mine site suggests that the proposed protected area is insufficient alone and should instead form part of a network. These results provide support for including hydrothermally active and inactive features within networks of protected areas and emphasise the need for quantitative survey data of proposed sites. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

A Massively Parallel Computational Method of Reading Index Files for SOAPsnv.

PubMed

Zhu, Xiaoqian; Peng, Shaoliang; Liu, Shaojie; Cui, Yingbo; Gu, Xiang; Gao, Ming; Fang, Lin; Fang, Xiaodong

2015-12-01

SOAPsnv is the software used for identifying the single nucleotide variation in cancer genes. However, its performance is yet to match the massive amount of data to be processed. Experiments reveal that the main performance bottleneck of SOAPsnv software is the pileup algorithm. The original pileup algorithm's I/O process is time-consuming and inefficient to read input files. Moreover, the scalability of the pileup algorithm is also poor. Therefore, we designed a new algorithm, named BamPileup, aiming to improve the performance of sequential read, and the new pileup algorithm implemented a parallel read mode based on index. Using this method, each thread can directly read the data start from a specific position. The results of experiments on the Tianhe-2 supercomputer show that, when reading data in a multi-threaded parallel I/O way, the processing time of algorithm is reduced to 3.9 s and the application program can achieve a speedup up to 100×. Moreover, the scalability of the new algorithm is also satisfying.

Deviations from a uniform period spacing of gravity modes in a massive star.

PubMed

Degroote, Pieter; Aerts, Conny; Baglin, Annie; Miglio, Andrea; Briquet, Maryline; Noels, Arlette; Niemczura, Ewa; Montalban, Josefina; Bloemen, Steven; Oreiro, Raquel; Vucković, Maja; Smolders, Kristof; Auvergne, Michel; Baudin, Frederic; Catala, Claude; Michel, Eric

2010-03-11

The life of a star is dominantly determined by the physical processes in the stellar interior. Unfortunately, we still have a poor understanding of how the stellar gas mixes near the stellar core, preventing precise predictions of stellar evolution. The unknown nature of the mixing processes as well as the extent of the central mixed region is particularly problematic for massive stars. Oscillations in stars with masses a few times that of the Sun offer a unique opportunity to disentangle the nature of various mixing processes, through the distinct signature they leave on period spacings in the gravity mode spectrum. Here we report the detection of numerous gravity modes in a young star with a mass of about seven solar masses. The mean period spacing allows us to estimate the extent of the convective core, and the clear periodic deviation from the mean constrains the location of the chemical transition zone to be at about 10 per cent of the radius and rules out a clear-cut profile.

Regulation of synaptic activity by snapin-mediated endolysosomal transport and sorting

PubMed Central

Di Giovanni, Jerome; Sheng, Zu-Hang

2015-01-01

Recycling synaptic vesicles (SVs) transit through early endosomal sorting stations, which raises a fundamental question: are SVs sorted toward endolysosomal pathways? Here, we used snapin mutants as tools to assess how endolysosomal sorting and trafficking impact presynaptic activity in wild-type and snapin−/− neurons. Snapin acts as a dynein adaptor that mediates the retrograde transport of late endosomes (LEs) and interacts with dysbindin, a subunit of the endosomal sorting complex BLOC-1. Expressing dynein-binding defective snapin mutants induced SV accumulation at presynaptic terminals, mimicking the snapin−/− phenotype. Conversely, over-expressing snapin reduced SV pool size by enhancing SV trafficking to the endolysosomal pathway. Using a SV-targeted Ca2+ sensor, we demonstrate that snapin–dysbindin interaction regulates SV positional priming through BLOC-1/AP-3-dependent sorting. Our study reveals a bipartite regulation of presynaptic activity by endolysosomal trafficking and sorting: LE transport regulates SV pool size, and BLOC-1/AP-3-dependent sorting fine-tunes the Ca2+ sensitivity of SV release. Therefore, our study provides new mechanistic insights into the maintenance and regulation of SV pool size and synchronized SV fusion through snapin-mediated LE trafficking and endosomal sorting. PMID:26108535

Heaviest Stellar Black Hole Discovered in Nearby Galaxy

NASA Astrophysics Data System (ADS)

2007-10-01

Astronomers have located an exceptionally massive black hole in orbit around a huge companion star. This result has intriguing implications for the evolution and ultimate fate of massive stars. The black hole is part of a binary system in M33, a nearby galaxy about 3 million light years from Earth. By combining data from NASA's Chandra X-ray Observatory and the Gemini telescope on Mauna Kea, Hawaii, the mass of the black hole, known as M33 X-7, was determined to be 15.7 times that of the Sun. This makes M33 X-7 the most massive stellar black hole known. A stellar black hole is formed from the collapse of the core of a massive star at the end of its life. Chandra X-ray Image of M33 X-7 Chandra X-ray Image of M33 X-7 "This discovery raises all sorts of questions about how such a big black hole could have been formed," said Jerome Orosz of San Diego State University, lead author of the paper appearing in the October 18th issue of the journal Nature. M33 X-7 orbits a companion star that eclipses the black hole every three and a half days. The companion star also has an unusually large mass, 70 times that of the Sun. This makes it the most massive companion star in a binary system containing a black hole. Hubble Optical Image of M33 X-7 Hubble Optical Image of M33 X-7 "This is a huge star that is partnered with a huge black hole," said coauthor Jeffrey McClintock of the Harvard-Smithsonian Center for Astrophysics in Cambridge, Mass. "Eventually, the companion will also go supernova and then we'll have a pair of black holes." The properties of the M33 X-7 binary system - a massive black hole in a close orbit around a massive companion star - are difficult to explain using conventional models for the evolution of massive stars. The parent star for the black hole must have had a mass greater than the existing companion in order to have formed a black hole before the companion star. Gemini Optical Image of M33 X-7 Gemini Optical Image of M33 X-7 Such a massive star would have had a radius larger than the present separation between the stars, so the stars must have been brought closer while sharing a common outer atmosphere. This process typically results in a large amount of mass being lost from the system, so much that the parent star should not have been able to form a 15.7 solar-mass black hole. The black hole's progenitor must have shed gas at a rate about 10 times less than predicted by models before it exploded. If even more massive stars also lose very little material, it could explain the incredibly luminous supernova seen recently as SN 2006gy. The progenitor for SN 2006gy is thought to have been about 150 times the mass of the Sun when it exploded. Artist's Illustration of M33 X-7 Artist's Illustration of M33 X-7 "Massive stars can be much less extravagant than people think by hanging onto a lot more of their mass toward the end of their lives," said Orosz. "This can have a big effect on the black holes that these stellar time-bombs make." Coauthor Wolfgang Pietsch was also the lead author of an article in the Astrophysical Journal that used Chandra observations to report that M33 X-7 is the first black hole in a binary system observed to undergo eclipses. The eclipsing nature enables unusually accurate estimates for the mass of the black hole and its companion. "Because it's eclipsing and because it has such extreme properties, this black hole is an incredible test-bed for studying astrophysics," said Pietsch. The length of the eclipse seen by Chandra gives information about the size of the companion. The scale of the companion's motion, as inferred from the Gemini observations, gives information about the mass of the black hole and its companion. Other observed properties of the binary were used to constrain the mass estimates. NASA's Marshall Space Flight Center, Huntsville, Ala., manages the Chandra program for the agency's Science Mission Directorate. The Smithsonian Astrophysical Observatory controls science and flight operations from the Chandra X-ray Center in Cambridge, Mass. Gemini is an international partnership managed by the Association of Universities for Research in Astronomy under a cooperative agreement with the National Science Foundation.

CellSort: a support vector machine tool for optimizing fluorescence-activated cell sorting and reducing experimental effort.

PubMed

Yu, Jessica S; Pertusi, Dante A; Adeniran, Adebola V; Tyo, Keith E J

2017-03-15

High throughput screening by fluorescence activated cell sorting (FACS) is a common task in protein engineering and directed evolution. It can also be a rate-limiting step if high false positive or negative rates necessitate multiple rounds of enrichment. Current FACS software requires the user to define sorting gates by intuition and is practically limited to two dimensions. In cases when multiple rounds of enrichment are required, the software cannot forecast the enrichment effort required. We have developed CellSort, a support vector machine (SVM) algorithm that identifies optimal sorting gates based on machine learning using positive and negative control populations. CellSort can take advantage of more than two dimensions to enhance the ability to distinguish between populations. We also present a Bayesian approach to predict the number of sorting rounds required to enrich a population from a given library size. This Bayesian approach allowed us to determine strategies for biasing the sorting gates in order to reduce the required number of enrichment rounds. This algorithm should be generally useful for improve sorting outcomes and reducing effort when using FACS. Source code available at http://tyolab.northwestern.edu/tools/ . k-tyo@northwestern.edu. Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

Prognostic Performance Evaluation of the International Society on Thrombosis and Hemostasis and the Korean Society on Thrombosis and Hemostasis Scores in the Early Phase of Trauma.

PubMed

Kim, Hong Sug; Lee, Dong Hun; Lee, Byung Kook; Cho, Yong Soo

2018-01-15

Disseminated intravascular coagulation (DIC) contributes to poor outcome in the early phase of trauma. We aimed to analyze and compare the prognostic performances of the International Society on Thrombosis and Hemostasis (ISTH) and the Korean Society on Thrombosis and Hemostasis (KSTH) scores in the early phase of trauma. Receiver operating characteristics analysis was used to examine the prognostic performance of both scores, and multivariate analysis was used to estimate the prognostic impact of the ISTH and KSTH scores in the early phase of trauma. The primary outcome was 24-hour mortality and the secondary outcome was massive transfusion. Of 1,229 patients included in the study, the 24-hour mortality rate was 7.6% (n = 93), and 8.1% (n = 99) of patients who received massive transfusions. The area under the curves (AUCs) of the KSTH and ISTH scores for 24-hour mortality were 0.784 (95% confidence interval [CI], 0.760-0.807) and 0.744 (95% CI, 0.718-0.768), respectively. The AUC of KSTH and ISTH scores for massive transfusion were 0.758 (95% CI, 0.734-0.782) and 0.646 (95% CI, 0.619-0.673), respectively. The AUCs of the KSTH score was significantly different from those of the ISTH score. Overt DIC according to KSTH criteria only, was independently associated with 24-hour mortality (odds ratio [OR], 2.630; 95% CI, 1.456-4.752). Only the KSTH score was independently associated with massive transfusion (OR, 1.563; 95% CI, 1.182-2.068). The KSTH score demonstrates a better prognostic performance for outcomes than the ISTH score in the early phase of trauma. © 2018 The Korean Academy of Medical Sciences.

Extreme Wolf-Rayet Galaxies with HST/COS: Understanding CIII] Emission in the Reionization Era

NASA Astrophysics Data System (ADS)

Stark, Daniel

2017-08-01

The first deep spectra of reionization-era galaxies have revealed strong UV nebular emission in high-ionization lines. This is in striking contrast to massive galaxies at lower redshifts, where emission from CIII], OIII], HeII, and CIV is rarely seen. These lines will likely be the only probe available for the most distant galaxies JWST will detect; but we are still unprepared to interpret them. Modeling predicts that intense UV nebular emission can only be produced below a tenth solar metallicity. However, recent HST/COS observations of local galaxies suggest that extreme populations of Wolf-Rayet (WR) stars, the hot exposed cores of massive O stars, may be capable of powering CIII] at metallicities as high as a half-solar. If these moderately metal-poor extreme WR galaxies are indeed a viable source of strong CIII] emission, our interpretation of CIII] detections in the reionization era will be dramatically altered; but we presently have sufficient UV coverage for only three examples. Here, we propose HST/COS G160M and G185M observations of an additional seven extreme WR galaxies spanning 0.5 dex in metallicity around half-solar. These observations will constrain the maximum CIII] equivalent width these galaxies can power as a function of metallicity. The moderate resolution gratings will robustly characterize the massive O and WR star populations, allowing us to link the nebular emission directly to the massive stars responsible. These data will provide a stringent test for the population synthesis codes which will be applied to JWST observations. Without this empirical baseline, our understanding of the most distant galaxies JWST finds will be severely limited.

An outcomes analysis of patients undergoing body contouring surgery after massive weight loss.

PubMed

Shermak, Michele A; Chang, David; Magnuson, Thomas H; Schweitzer, Michael A

2006-09-15

Although published reports about technical management of massive weight loss patients are beginning to appear, risk factors for complications following body contouring operations are not known. A retrospective analysis of massive weight loss patients who had body contouring operations between March of 1998 and October of 2004 was performed. Demographic and surgical factors were analyzed. Outcome measures included seroma, wound dehiscence, thromboembolic complications, blood transfusion after surgery, and extended lengths of stay (>2 days). A total of 139 patients were analyzed; 82.7 percent of them were female; mean age was 41 years. On multiple logistic regression, male gender was associated with significant risks for wound dehiscence (odds ratio, 6.4; p = 0.01). There were also trends toward increased risk for wound dehiscence with hypothyroidism (odds ratio, 4.3; p = 0.06) and Ehlers-Danlos syndrome (odds ratio, 18.7; p = 0.05). In terms of risk of blood transfusion, asthma and having three or more procedures were the two variables that emerged with significant association (odds ratio, 16.8 and 13.7, respectively; both p < 0.01). Increased length of stay to greater than 2 days was also significantly associated with having three or more procedures (odds ratio, 4.72; p < 0.01). Male gender, hypothyroidism, and Ehlers-Danlos syndrome may be risk factors for wound dehiscence following body contour operations for massive weight loss. Asthma may be a marker of poor general health status, and asthmatic patients are at increased risk for requiring blood transfusions. Having three or more procedures is associated with an increased risk of blood transfusion and increased length of stay.

Categorizing Variations of Student-Implemented Sorting Algorithms

ERIC Educational Resources Information Center

Taherkhani, Ahmad; Korhonen, Ari; Malmi, Lauri

2012-01-01

In this study, we examined freshmen students' sorting algorithm implementations in data structures and algorithms' course in two phases: at the beginning of the course before the students received any instruction on sorting algorithms, and after taking a lecture on sorting algorithms. The analysis revealed that many students have insufficient…

Review of log sort yards

Treesearch

John Rusty Dramm; Gerry L. Jackson; Jenny Wong

2002-01-01

This report provides a general overview of current log sort yard operations in the United States, including an extensive literature review and information collected during on-site visits to several operations throughout the nation. Log sort yards provide many services in marketing wood and fiber by concentrating, merchandising, processing, sorting, and adding value to...

COST EVALUATION OF AUTOMATED AND MANUAL POST- CONSUMER PLASTIC BOTTLE SORTING SYSTEMS

EPA Science Inventory

This project evaluates, on the basis of performance and cost, two Automated BottleSort® sorting systems for post-consumer commingled plastic containers developed by Magnetic Separation Systems. This study compares the costs to sort mixed bales of post-consumer plastic at these t...

Origin and character of loesslike silt in unglaciated south-central Yakutia, Siberia, U.S.S.R.

USGS Publications Warehouse

Pewe, Troy Lewis; Journaux, Andre

1983-01-01

Loesslike silt mantles upland terraces and low plateaus throughout unglaciated south-central Yakutia but is thickest along the south side of the lower Aldan River valley and the east side of the Lena River valley. The silt is probably loess deposited during glacial advances by winds blowing southward from the Verkhoyansk Range and eastward across the broad vegetation-free flood plain of the braided Lena River. The well-sorted uniform tan silt is well displayed along the Aldan and Lena Rivers; the thickest exposure measured, more than 60 m, is on the Tyungyulyu Terrace on the east side of the Lena River. On the west side of the valley, it is 10-25 m thick but thins rapidly to a featheredge west of Yakutsk. Almost all scarps along the south side of the Aldan River are capped by 10-35 m of silt. The texture and mineral composition of the loess like silt are uniform throughout south-central Yakutia, whether it overlies limestone, poorly consolidated sandstone, alluvium, and glacial outwash. All silt samples examined contained a high percentage of quartz and feldspar. The silt stands in sheer cliffs and is massive, with little or no stratification. The origin of the loesslike silt has been ascribed to disintegration in place of country rock by a marine, estuarine, lacustrine, fluvial, residual, or eolian source, or to a combination of these processes. The marine and estuarine hypotheses have never had strong support, but the lacustrine, fluvial, and residual hypotheses have been advanced by many Soviet workers. The most widely accepted explanation of the origin of the upland silt is that it is a combination of lacustrine and alluvial deposits formed on great flood plains and marshy plains. This origin is unlikely, however, because there are no shorelines, wave-cut beaches, deltas, mudcracks, or ripple marks. Neither distinct stratification nor an appreciable amount of clay exists in the silt. Moreover, there is no definite upper boundary to the deposits, as would be expected of lacustrine deposits. The loesslike silt has also been described as a residual deposit formed by the breakdown, by freezing and thawing, of the underlying rocks. The silt bears no chemical, mineralogic, or textural relation to the underlying strata, however, and it is too thick to represent only breakdown of rocks in place. The widespread mantle of uniform loesslike silt is here considered to be windblown, derived from glacial outwash in braided streams and on broad plains, because: (1) it occurs as a surficial mantle; (2) it is lithologically independent of the underlying material; (3) it is stratified indistinctly or not at all, except in retransported material; (4) it is associated with sand dunes; (5) it contains fossils of land animals; (6) its sorting and texture are similar to that of loess and windblown dust from many places elsewhere in the world; (7) its grains are angular and relatively unweathered.

Taxonomic Notes on Nasutitermes and Bulbitermes (Termitidae, Nasutitermitinae) from the Sunda region of Southeast Asia based on morphological and molecular characters

PubMed Central

Syaukani; Thompson, Graham J.

2011-01-01

Abstract The Sunda region of Southeastern Asia is rich in termite fauna, but termites from this region have been poorly described. In this study, we described eight species from two diverse genera from this region, and from the family Termitidae. We describe Bulbitermes 4 spp. and Nasutitermes 4 spp. from new field collections. Where possible we examine original holotype specimens, and describe the essential morphological characters for soldier and worker castes. We devise two new bifurcating keys to guide the field identification of each species. In addition, we develop a nucleotide sequence profile for the COI gene. From this molecular character matrix, we use Neighbour-Joining analysis to test the monophyly of each morphospecies and genus. We find that the morphological and molecular characters are highly concordant, whereby all taxa appear to represent distinct molecular clades. For termites, there is therefore agreement between the morphological taxonomic characters used to sort species from a bifurcating key and the molecular taxonomic characters used to sort species on a bifurcating tree. This joint analysis suggests that DNA barcoding holds considerable promise for termite taxonomy, especially for diverse clades like Bulbitermes and Nasutitermes for which a global morphological key would be intractable. PMID:22287894

Erosion and deposition produced by the flood of December 1964 on Coffee Creek, Trinity County, California

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stewart, J.H.; LaMarrhe, V.C. Jr.

1967-01-01

The flood was unprecedented in the 110-yr period since settlement of the area and had a catastrophic effect on the valley of Coffee Creek. Erosion destroyed large areas of meadowland and forests containing 200-yr old trees and many of the buildings and structures on the valley bottom. Deposits of sand and poorly sorted gravel laid down during the flood cover at least 70% of the flooded area. The largest boulder transported was approximately 6 x 4 x 3 ft. Natural levees formed during the flood occur along the sides of the main flood channels. These levees are composed of coarsemore » bouldery gravel, are generally 30-50 ft wide, and slope gently away from the flood channel. Along much of the valley the amount of material lost from an area where the postflood surface is below the preflood surface (net scour) tends to be matched by a corresponding gain of material in nearby areas where the postflood surface is above the preflood surface (net fill). The effect of the 1964 flood on Coffee Creek clearly indicates that it is catastrophic events of this sort that largely determine valley morphology, channel pattern and location, and the character of alluvial deposits. (24 refs.)« less

Cognitive flexibility: A trait of bipolar disorder that worsens with length of illness.

PubMed

O'Donnell, Lisa A; Deldin, Patricia J; Pester, Bethany; McInnis, Melvin G; Langenecker, Scott A; Ryan, Kelly A

2017-12-01

Deficits in cognitive flexibility, a difficulty altering thoughts and behavioral responses in a changing environment, are found in individuals with bipolar disorder (BD) and are associated with poor social and work functioning. However, the current literature is inconsistent in clarifying the long-term nature of these deficits for those with BD. We administered a common task of cognitive flexibility, the Wisconsin Card Sorting Task (WCST) and accounted for demographics, clinical, and cognitive features of BD, to determine the state versus trait characteristics of these deficits. The Wisconsin Card Sorting Test (WCST) was administered to 154 adults with BD and 95 healthy controls twice, one year apart. The main findings show that cognitive inflexibility is a trait feature of BD, independent of clinical features, that may modestly worsen over time due to the presence of certain demographic, cognitive, and functional features of the disorder. In addition, improvements in WCST performance over an extended period of time in both those with and those without already existing cognitive flexibility deficits indicate potential practice effects. These findings suggest that the implementation of early interventions before the illness progresses could potentially prevent further cognitive impairment, mitigate functional outcomes, and improve the quality of life of the individual with BD.

Testing a "content meets process" model of depression vulnerability and rumination: Exploring the moderating role of set-shifting deficits.

PubMed

Vergara-Lopez, Chrystal; Lopez-Vergara, Hector I; Roberts, John E

2016-03-01

MacCoon and Newman's (2006) "content meets process" model posits that deficits in cognitive control make it difficult to disengage from negative cognitions caused by a negative cognitive style (NCS). The present study examined if the interactive effect of cognitive set-shifting abilities and NCS predicts rumination and past history of depression. Participants were 90 previously depressed individuals and 95 never depressed individuals. We administered three laboratory tasks that assess set-shifting: the Wisconsin Card-Sorting Task, the Emotional Card-Sorting Task, and the Internal Switch Task, and self-report measures of NCS and rumination. Shifting ability in the context of emotional distractors moderated the association between NCS and depressive rumination. Although previously depressed individuals had more NCS and higher trait rumination relative to never depressed individuals, shifting ability did not moderate the association between NCS and depression history. The cross-sectional correlational design cannot address the causal direction of effects. It is also not clear whether findings will generalize beyond college students. NCS was elevated in previously depressed individuals consistent with its theoretical role as trait vulnerability to the disorder. Furthermore, NCS may be particularly likely to trigger rumination among individuals with poor capacity for cognitive control in the context of emotional distraction. Copyright © 2015 Elsevier Ltd. All rights reserved.

Effective personal protective clothing for health care workers attending patients with severe acute respiratory syndrome.

PubMed

Wong, Thomas K S; Chung, Joanne W Y; Li, Y; Chan, Wai F; Ching, Patricia T Y; Lam, Conita H S; Chow, Chun B; Seto, Wing H

2004-04-01

Optimal usability is crucial in providing protection for health care workers who are exposed to severe acute respiratory syndrome day and night while taking care of patients with the virus. No research study has yet tested the usability of personal protective clothing (PPC). The study was carried out in 3 stages. PPC available in Hong Kong were sorted by their physical properties in the first stage. The second stage was a single-blinded study examining the different usability aspects of the PPC. The third stage was a simulated viral load test. Four types were identified: good water repellency and water resistance, poor air permeability (Type A PPC); good water repellency and air permeability, poor water resistance (Type B PPC); poor water repellency, poor water resistance, and fair air permeability (Type C PPC); and good water repellency, poor air permeability, and fair water resistance (Type D PPC). Type D PPC had a significantly higher number of contamination sites on the subjects' dorsum and palm. Type C PPC had the highest contamination over the trunk. Findings in the viral load test showed that there was a significant difference in the contamination of the face (t=4.69, df=38, P<.00) between 1 and 2 strokes. Type A PPC is effective in providing a desirable protective function against droplet splash, if a disposable PPC is required. Type C PPC, the surgical gown, is also appropriate, as the cost is low, air permeability is fair, and the level of possible hand contamination is lowest among the 4 groups in the current study.

Friction in debris flows: inferences from large-scale flume experiments

USGS Publications Warehouse

Iverson, Richard M.; LaHusen, Richard G.; ,

1993-01-01

A recently constructed flume, 95 m long and 2 m wide, permits systematic experimentation with unsteady, nonuniform flows of poorly sorted geological debris. Preliminary experiments with water-saturated mixtures of sand and gravel show that they flow in a manner consistent with Coulomb frictional behavior. The Coulomb flow model of Savage and Hutter (1989, 1991), modified to include quasi-static pore-pressure effects, predicts flow-front velocities and flow depths reasonably well. Moreover, simple scaling analyses show that grain friction, rather than liquid viscosity or grain collisions, probably dominates shear resistance and momentum transport in the experimental flows. The same scaling indicates that grain friction is also important in many natural debris flows.

Use of carboxylated microspheres to assess transport potential of Cryptosporidium parvum oocysts at the Russian River water supply facility, Sonoma County, California

USGS Publications Warehouse

Metge, D.W.; Harvey, R.W.; Anders, R.; Rosenberry, D.O.; Seymour, D.; Jasperse, James

2007-01-01

Carboxylated microspheres were employed as surrogates to assess the transport potential of Cryptosporidium parvumoocysts during forced- and natural-gradient tests conducted in July and October 2004. The tests involved poorly-sorted, near-surface sediments where groundwater is pumped from an alluvial aquifer underlying the Russian River, Sonoma County, CA. In an off channel infiltration basin and within the river, a mixture (2-, 3-, and 5- ??m diameters) of fluorescently-labeled carboxylated microspheres and bromide tracers were used in two injection and recovery test to assess sediment removal efficiency for the microspheres. Bottom sediments varied considerably in their filtration efficiency for Cryptosporidium.

A million dollar exit from the anarchic slum-world: Slumdog Millionaire's hollow idioms of social justice.

PubMed

Sengupta, Mitu

2010-01-01

This article contests the characterisation of the popular and acclaimed film, Slumdog Millionaire, as a realistic portrayal of India's urban poverty that will ultimately serve as a tool of advocacy for India's urban poor. It argues that the film's reductive view of slum-spaces will more probably reinforce negative attitudes towards slum-dwellers, lending credibility to the sorts of policies that have historically dispossessed them of power and dignity. By drawing attention to the film's celebration of characters and spaces that symbolise Western culture and Northern trajectories of 'development', the article also critically engages with some of the issues raised by the film's enormous success.

An investigation of constructions of justice and injustice in chronic pain: a Q-methodology approach.

PubMed

McParland, Joanna; Hezseltine, Louisa; Serpell, Michael; Eccleston, Christopher; Stenner, Paul

2011-09-01

This study used Q-methodology to explore justice-related accounts of chronic pain. Eighty participants completed the Q-sorting procedure (33 chronic pain sufferers and 47 non-pain sufferers). Analysis revealed five main factors. Three factors blame: society for poor medical and interpersonal treatment; the chronic pain sufferer for indulging in self-pity and unempathic healthcare workers for ignoring patients. A fourth factor acknowledges the unfairness of pain and encourages self-reliance. The fifth factor rejects injustice in the chronic pain discourse. Overall, there is a shared view that chronic pain brings unfair treatment, disrespect and a de-legitimization of pain. Future research ideas are suggested.

Einstein-Podolsky-Rosen paradox and quantum steering in pulsed optomechanics

NASA Astrophysics Data System (ADS)

He, Q. Y.; Reid, M. D.

2013-11-01

We describe how to generate an Einstein-Podolsky-Rosen (EPR) paradox between a mesoscopic mechanical oscillator and an optical pulse. We find two types of paradox, defined by whether it is the oscillator or the pulse that shows the effect Schrödinger called “steering”. Only the oscillator paradox addresses the question of mesoscopic local reality for a massive system. In that case, EPR's “elements of reality” are defined for the oscillator, and it is these elements of reality that are falsified (if quantum mechanics is complete). For this sort of paradox, we show that a thermal barrier exists, meaning that a threshold level of pulse-oscillator interaction is required for a given thermal occupation n0 of the oscillator. We find there is no equivalent thermal barrier for the entanglement of the pulse with the oscillator or for the EPR paradox that addresses the local reality of the optical system. Finally, we examine the possibility of an EPR paradox between two entangled oscillators. Our work highlights the asymmetrical effect of thermal noise on quantum nonlocality.

Human genetic resistance to malaria.

PubMed

Williams, Thomas N

2009-01-01

This brief chapter highlights the need for caution when designing and interpreting studies aimed at seeking new genes that may be associated with malaria protection, or investigating the potential mechanisms for protection in promising candidates. Judging genetic effects on the basis of the wrong clinical phenotype and missing true protective genes because their protective effects are masked by unpredictable epistatic effects are major potential pitfalls. These issues are by no means unique to malaria: in recent years, the importance of larger sample sizes and careful phenotypic definitions have become appreciated increasingly, particularly for genome-wide studies of complex diseases (Cordell and Clayton, 2005; Burton, Tobin and Hopper, 2005). Until recently, research in the field of malaria genetics has not enjoyed the sort of funding afforded to similar work investigating diseases of importance to the developed world. However, in the last few years, coupled with advances in genetic diagnostics that have led to massive automation and falling costs per gene explored, momentum has grown towards more generous funding that brings with it the opportunity for much larger, multisite cohesive studies. The stage is set for a giant leap forward in the coming years.

Climatically driven biogeographic provinces of Late Triassic tropical Pangea

PubMed Central

Whiteside, Jessica H.; Grogan, Danielle S.; Olsen, Paul E.; Kent, Dennis V.

2011-01-01

Although continents were coalesced into the single landmass Pangea, Late Triassic terrestrial tetrapod assemblages are surprisingly provincial. In eastern North America, we show that assemblages dominated by traversodont cynodonts are restricted to a humid 6° equatorial swath that persisted for over 20 million years characterized by “semiprecessional” (approximately 10,000-y) climatic fluctuations reflected in stable carbon isotopes and sedimentary facies in lacustrine strata. More arid regions from 5–20°N preserve procolophonid-dominated faunal assemblages associated with a much stronger expression of approximately 20,000-y climatic cycles. In the absence of geographic barriers, we hypothesize that these variations in the climatic expression of astronomical forcing produced latitudinal climatic zones that sorted terrestrial vertebrate taxa, perhaps by excretory physiology, into distinct biogeographic provinces tracking latitude, not geographic position, as the proto-North American plate translated northward. Although the early Mesozoic is usually assumed to be characterized by globally distributed land animal communities due to of a lack of geographic barriers, strong provinciality was actually the norm, and nearly global communities were present only after times of massive ecological disruptions. PMID:21571639

Climatically driven biogeographic provinces of Late Triassic tropical Pangea.

PubMed

Whiteside, Jessica H; Grogan, Danielle S; Olsen, Paul E; Kent, Dennis V

2011-05-31

Although continents were coalesced into the single landmass Pangea, Late Triassic terrestrial tetrapod assemblages are surprisingly provincial. In eastern North America, we show that assemblages dominated by traversodont cynodonts are restricted to a humid 6° equatorial swath that persisted for over 20 million years characterized by "semiprecessional" (approximately 10,000-y) climatic fluctuations reflected in stable carbon isotopes and sedimentary facies in lacustrine strata. More arid regions from 5-20 °N preserve procolophonid-dominated faunal assemblages associated with a much stronger expression of approximately 20,000-y climatic cycles. In the absence of geographic barriers, we hypothesize that these variations in the climatic expression of astronomical forcing produced latitudinal climatic zones that sorted terrestrial vertebrate taxa, perhaps by excretory physiology, into distinct biogeographic provinces tracking latitude, not geographic position, as the proto-North American plate translated northward. Although the early Mesozoic is usually assumed to be characterized by globally distributed land animal communities due to of a lack of geographic barriers, strong provinciality was actually the norm, and nearly global communities were present only after times of massive ecological disruptions.

A Massive-born Neutron Star with a Massive White Dwarf Companion

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cognard, Ismaël; Guillemot, Lucas; Theureau, Gilles

We report on the results of a 4 year timing campaign of PSR J2222−0137, a 2.44 day binary pulsar with a massive white dwarf (WD) companion, with the Nançay, Effelsberg, and Lovell radio telescopes. Using the Shapiro delay for this system, we find a pulsar mass m {sub p} = 1.76 ± 0.06 M {sub ⊙} and a WD mass m {sub c} = 1.293 ± 0.025 M {sub ⊙}. We also measure the rate of advance of periastron for this system, which is marginally consistent with the general relativity prediction for these masses. The short lifetime of the massivemore » WD progenitor star led to a rapid X-ray binary phase with little (< 10{sup −2} M {sub ⊙}) mass accretion onto the neutron star; hence, the current pulsar mass is, within uncertainties, its birth mass, which is the largest measured to date. We discuss the discrepancy with previous mass measurements for this system; we conclude that the measurements presented here are likely to be more accurate. Finally, we highlight the usefulness of this system for testing alternative theories of gravity by tightly constraining the presence of dipolar radiation. This is of particular importance for certain aspects of strong-field gravity, like spontaneous scalarization, since the mass of PSR J2222−0137 puts that system into a poorly tested parameter range.« less

No prosthetic management of massive and irreparable rotator cuff tears

PubMed Central

Garofalo, Raffaele; Cesari, Eugenio

2014-01-01

A massive rotator cuff tear is not necessarily irreparable. Number of tendons involved, muscle-tendon unit quality, and decreased acromionhumeral distance (AHD) are as important as tear size in determining reparability of lesion. Massive and irreparable rotator cuff tears cannot be anatomically repaired to the bone and are a common source of pain and disability even in middle-aged patients. In these patients when conservative management has failed, it is possible to perform different surgical techniques. A functional repair can help to restore the horizontal force couple of the cuff on the humeral head and to increase the AHD. Debridement of irreparable tears and biceps tenotomy or tenodesis can have a role in low functional demand patients but results deteriorate over time. Recently, several commercially available tissue-engineered biological and synthetic scaffolds have been developed to augment rotator cuff repairs. The aim is to provide a mechanical improvement in case of poor quality tissue at time zero and give a support to have a better cuff healing. In selected cases, the scaffold can be used also to bridge tendon defect. Patients who not have pseudoparalysis, cuff tear arthropathy and with intact deltoid function can benefit from tendon transfers with satisfactory outcomes. These different procedures should be chosen for each patient with selected criteria and after a satisfactory explanation about the really possible expectation after surgery. PMID:27582930

Quark-novae Occurring in Massive Binaries : A Universal Energy Source in Superluminous Supernovae with Double-peaked Light Curves

NASA Astrophysics Data System (ADS)

Ouyed, Rachid; Leahy, Denis; Koning, Nico

2016-02-01

A quark-nova (QN; the sudden transition from a neutron star into a quark star), which occurs in the second common envelope (CE) phase of a massive binary, gives excellent fits to superluminous, hydrogen-poor, supernovae (SLSNe) with double-peaked light curves, including DES13S2cmm, SN 2006oz, and LSQ14bdq (http://www.quarknova.ca/LCGallery.html). In our model, the H envelope of the less massive companion is ejected during the first CE phase, while the QN occurs deep inside the second, He-rich, CE phase after the CE has expanded in size to a radius of a few tens to a few thousands of solar radii; this yields the first peak in our model. The ensuing merging of the quark star with the CO core leads to black hole formation and accretion, explaining the second long-lasting peak. We study a sample of eight SLSNe Ic with double-humped light curves. Our model provides good fits to all of these, with a universal explosive energy of 2 × 1052 erg (which is the kinetic energy of the QN ejecta) for the first hump. The late-time emissions seen in iPTF13ehe and LSQ14bdq are fit with a shock interaction between the outgoing He-rich (I.e., second) CE and the previously ejected H-rich (I.e., first) CE.

Pre-supernova outbursts via wave heating in massive stars - II. Hydrogen-poor stars

NASA Astrophysics Data System (ADS)

Fuller, Jim; Ro, Stephen

2018-05-01

Pre-supernova (SN) outbursts from massive stars may be driven by hydrodynamical wave energy emerging from the core of the progenitor star during late nuclear-burning phases. Here, we examine the effects of wave heating in stars containing little or no hydrogen, i.e. progenitors of Type IIb/Ib SNe. Because there is no massive hydrogen envelope, wave energy is thermalized near the stellar surface where the overlying atmospheric mass is small but the optical depth is large. Wave energy can thus unbind this material, driving an optically thick, super-Eddington wind. Using 1D hydrodynamic MESA simulations of ˜5 M⊙ He stars, we find that wave heating can drive pre-SN outbursts composed of a dense wind whose mass-loss rate can exceed ˜0.1 M⊙ yr-1. The wind terminal velocities are a few 100 km s-1, and outburst luminosities can reach ˜106 L⊙. Wave-driven outbursts may be linked with observed or inferred pre-SN outbursts of Type Ibn/transitional/transformational SNe, and pre-SN wave-driven mass loss is a good candidate to produce these types of SNe. However, we also show that non-linear wave breaking in the core of the star may prevent such outbursts in stars with thick convective helium-burning shells. Hence, only a limited subset of SN progenitors is likely to experience wave-driven pre-SN outbursts.

Application of visible spectroscopy in waste sorting

NASA Astrophysics Data System (ADS)

Spiga, Philippe; Bourely, Antoine

2011-10-01

Today, waste recycling, (bottles, papers...), is a mechanical operation: the waste are crushed, fused and agglomerated in order to obtain new manufactured products (e.g. new bottles, clothes ...). The plastics recycling is the main application in the color sorting process. The colorless plastics recovered are more valuable than the colored plastics. Other emergent applications are in the paper sorting, where the main goal is to sort dyed paper from white papers. Up to now, Pellenc Selective Technologies has manufactured color sorting machines based on RGB cameras. Three dimensions (red, green and blue) are no longer sufficient to detect low quantities of dye in the considered waste. In order to increase the efficiency of the color detection, a new sorting machine, based on visible spectroscopy, has been developed. This paper presents the principles of the two approaches and their difference in terms of sorting performance, making visible spectroscopy a clear winner.

MetaSort untangles metagenome assembly by reducing microbial community complexity

PubMed Central

Ji, Peifeng; Zhang, Yanming; Wang, Jinfeng; Zhao, Fangqing

2017-01-01

Most current approaches to analyse metagenomic data rely on reference genomes. Novel microbial communities extend far beyond the coverage of reference databases and de novo metagenome assembly from complex microbial communities remains a great challenge. Here we present a novel experimental and bioinformatic framework, metaSort, for effective construction of bacterial genomes from metagenomic samples. MetaSort provides a sorted mini-metagenome approach based on flow cytometry and single-cell sequencing methodologies, and employs new computational algorithms to efficiently recover high-quality genomes from the sorted mini-metagenome by the complementary of the original metagenome. Through extensive evaluations, we demonstrated that metaSort has an excellent and unbiased performance on genome recovery and assembly. Furthermore, we applied metaSort to an unexplored microflora colonized on the surface of marine kelp and successfully recovered 75 high-quality genomes at one time. This approach will greatly improve access to microbial genomes from complex or novel communities. PMID:28112173

Ubiquitin-dependent sorting of integral membrane proteins for degradation in lysosomes

PubMed Central

Piper, Robert C.

2007-01-01

Summary The pathways that deliver newly synthesized proteins that reside in lysosomes are well understood by comparison with our knowledge of how integral membrane proteins are sorted and delivered to the lysosome for degradation. Many membrane proteins are sorted to lysosomes following ubiquitination, which provides a sorting signal that can operate for sorting at the TGN (trans-Golgi network), at the plasma membrane or at the endosome for delivery into lumenal vesicles. Candidate multicomponent machines that can potentially move ubiquitinated integral membrane cargo proteins have been identified, but much work is still required to ascertain which of these candidates directly recognizes ubiquitinated cargo and what they do with cargo after recognition. In the case of the machinery required for sorting into the lumenal vesicles of endosomes, other functions have also been determined including a link between sorting and movement of endosomes along microtubules. PMID:17689064

The Stars behind the Curtain

NASA Astrophysics Data System (ADS)

2010-02-01

ESO is releasing a magnificent VLT image of the giant stellar nursery surrounding NGC 3603, in which stars are continuously being born. Embedded in this scenic nebula is one of the most luminous and most compact clusters of young, massive stars in our Milky Way, which therefore serves as an excellent "local" analogue of very active star-forming regions in other galaxies. The cluster also hosts the most massive star to be "weighed" so far. NGC 3603 is a starburst region: a cosmic factory where stars form frantically from the nebula's extended clouds of gas and dust. Located 22 000 light-years away from the Sun, it is the closest region of this kind known in our galaxy, providing astronomers with a local test bed for studying intense star formation processes, very common in other galaxies, but hard to observe in detail because of their great distance from us. The nebula owes its shape to the intense light and winds coming from the young, massive stars which lift the curtains of gas and clouds revealing a multitude of glowing suns. The central cluster of stars inside NGC 3603 harbours thousands of stars of all sorts (eso9946): the majority have masses similar to or less than that of our Sun, but most spectacular are several of the very massive stars that are close to the end of their lives. Several blue supergiant stars crowd into a volume of less than a cubic light-year, along with three so-called Wolf-Rayet stars - extremely bright and massive stars that are ejecting vast amounts of material before finishing off in glorious explosions known as supernovae. Using another recent set of observations performed with the SINFONI instrument on ESO's Very Large Telescope (VLT), astronomers have confirmed that one of these stars is about 120 times more massive than our Sun, standing out as the most massive star known so far in the Milky Way [1]. The clouds of NGC 3603 provide us with a family picture of stars in different stages of their life, with gaseous structures that are still growing into stars, newborn stars, adult stars and stars nearing the end of their life. All these stars have roughly the same age, a million years, a blink of an eye compared to our five billion year-old Sun and Solar System. The fact that some of the stars have just started their lives while others are already dying is due to their extraordinary range of masses: high-mass stars, being very bright and hot, burn through their existence much faster than their less massive, fainter and cooler counterparts. The newly released image, obtained with the FORS instrument attached to the VLT at Cerro Paranal, Chile, portrays a wide field around the stellar cluster and reveals the rich texture of the surrounding clouds of gas and dust. Notes [1] The star, NGC 3603-A1, is an eclipsing system of two stars orbiting around each other in 3.77 days. The most massive star has an estimated mass of 116 solar masses, while its companion has a mass of 89 solar masses. More information ESO, the European Southern Observatory, is the foremost intergovernmental astronomy organisation in Europe and the world's most productive astronomical observatory. It is supported by 14 countries: Austria, Belgium, the Czech Republic, Denmark, France, Finland, Germany, Italy, the Netherlands, Portugal, Spain, Sweden, Switzerland and the United Kingdom. ESO carries out an ambitious programme focused on the design, construction and operation of powerful ground-based observing facilities enabling astronomers to make important scientific discoveries. ESO also plays a leading role in promoting and organising cooperation in astronomical research. ESO operates three unique world-class observing sites in Chile: La Silla, Paranal and Chajnantor. At Paranal, ESO operates the Very Large Telescope, the world's most advanced visible-light astronomical observatory and VISTA, the largest survey telescope. ESO is the European partner of a revolutionary astronomical telescope ALMA, the largest astronomical project in existence. ESO is currently planning a 42-metre European Extremely Large optical/near-infrared Telescope, the E-ELT, which will become "the world's biggest eye on the sky".

Is it time to revisit the log-sort yard?

Treesearch

John Dramm; Gerry Jackson

2000-01-01

Log-sort yards provide better utilization and marketing with improved value recovery of currently available timber resources in North America. Log-sort yards provide many services in marketing wood and fiber by concentrating, merchandising, manufacturing, sorting, and adding value to logs. Such operations supply forest products firms with desired raw materials, which...

Word Sorts for General Music Classes

ERIC Educational Resources Information Center

Cardany, Audrey Berger

2015-01-01

Word sorts are standard practice for aiding children in acquiring skills in English language arts. When included in the general music classroom, word sorts may aid students in acquiring a working knowledge of music vocabulary. The author shares a word sort activity drawn from vocabulary in John Lithgow's children's book "Never Play…

The effects of hoechst 33342 staining and the male sample donor on the sorting efficiency of canine spermatozoa.

PubMed

Rodenas, C; Lucas, X; Tarantini, T; Del Olmo, D; Roca, J; Vazquez, J M; Martinez, E A; Parrilla, I

2014-02-01

The aim of this study was to evaluate the influence of Hoechst 33342 (H-42) concentration and of the male donor on the efficiency of sex-sorting procedure in canine spermatozoa. Semen samples from six dogs (three ejaculates/dog) were diluted to 100 × 10(6) sperm/ml, split into four aliquots, stained with increasing H-42 concentrations (5, 7.5, 10 and 12.5 μl, respectively) and sorted by flow cytometry. The rates of non-viable (FDA+), oriented (OS) and selected spermatozoa (SS), as well as the average sorting rates (SR, sorted spermatozoa/s), were used to determine the sorting efficiency. The effects of the sorting procedure on the quality of sorted spermatozoa were evaluated in terms of total motility (TM), percentage of viable spermatozoa (spermatozoa with membrane and acrosomal integrity) and percentage of spermatozoa with reacted/damaged acrosomes. X- and Y-chromosome-bearing sperm populations were identified in all of the samples stained with 7.5, 10 and 12.5 μl of H-42, while these two populations were only identified in 77.5% of samples stained with 5 μl. The values of OS, SS and SR were influenced by the male donor (p < 0.01) but not by the H-42 concentration used. The quality of sorted sperm samples immediately after sorting was similar to that of fresh samples, while centrifugation resulted in significant reduction (p < 0.05) in TM and in the percentage of viable spermatozoa and a significant increase (p < 0.01) in the percentage of spermatozoa with damage/reacted acrosomes. In conclusion, the sex-sorting of canine spermatozoa by flow cytometry can be performed successfully using H-42 concentrations between 7.5 and 12.5 μl. The efficiency of the sorting procedure varies based on the dog from which the sperm sample derives. © 2013 Blackwell Verlag GmbH.

The Age of the First Nucleosynthesis in the Galaxy

NASA Astrophysics Data System (ADS)

Cayrel, R.; Hill, V.; Spite, M.; Spite, F.; François, P.; Depagne, E.; Nordström, B.; Andersen, J.; Plez, B.; Barbuy, B.; Beers, T.; Bonifacio, P.; Molaro, P.; Primas, F.

The observation of extremely metal-poor stars makes possible to obtain the epoch of formation of the first elements produced by massive supernovae, or hypernovae. The classical way of obtaining stellar ages (isochrone fitting) is unfortunately not applicable at present, as the distances of these stars are not known with enough accuracy. Dating by decay of radio-elements is another path, which has been renewed by the observation of uranium in the yellow giant CS 31082-001. We discuss the prospects opened by this discovery, and recent theoretical works triggered by this new opportunity.

Searching for decaying axionlike dark matter from clusters of galaxies.

PubMed

Riemer-Sørensen, Signe; Zioutas, Konstantin; Hansen, Steen H; Pedersen, Kristian; Dahle, Håkon; Liolios, Anastasios

2007-09-28

We constrain the lifetime of radiatively decaying dark matter in clusters of galaxies inspired by generic Kaluza-Klein axions, which have been invoked as a possible explanation for the solar coronal x-ray emission. These particles can be produced inside stars and remain confined by the gravitational potential of clusters. By analyzing x-ray observations of merging clusters, where gravitational lensing observations have identified massive, baryon poor structures, we derive the first cosmological lifetime constraint on this kind of particles of tau > or = 10(23) sec.

Sorting out Ideas about Function

ERIC Educational Resources Information Center

Hillen, Amy F.; Malik, LuAnn

2013-01-01

Card sorting has the potential to provide opportunities for exploration of a variety of topics and levels. In a card-sorting task, each participant is presented with a set of cards--each of which depicts a relationship--and is asked to sort the cards into categories that make sense to him or her. The concept of function is critical to…

Gender Sorting across K-12 Schools in the United States

ERIC Educational Resources Information Center

Long, Mark C.; Conger, Dylan

2013-01-01

This article documents evidence of nonrandom gender sorting across K-12 schools in the United States. The sorting exists among coed schools and at all grade levels, and it is highest in the secondary school grades. We observe some gender sorting across school sectors and types: for instance, males are slightly underrepresented in private schools…

Lazarus's BASIC ID: Making Initial Client Assessments Using Q-Sorts.

ERIC Educational Resources Information Center

Miller, Mark J.

1987-01-01

Presents overview of Lazarus's multimodal therapy model and the Q-sort, an observer-evaluation scoring instrument. Outlines feasibility of integrating Q-sort within multimodal model. Describes both a preliminary attempt using expert raters to categorize Q-sort cards within the model and a case study on how to assess client by incorporating Q-sort…

Machine Vision System for Color Sorting Wood Edge-Glued Panel Parts

Treesearch

Qiang Lu; S. Srikanteswara; W. King; T. Drayer; Richard Conners; D. Earl Kline; Philip A. Araman

1997-01-01

This paper describes an automatic color sorting system for hardwood edge-glued panel parts. The color sorting system simultaneously examines both faces of a panel part and then determines which face has the "better" color given specified color uniformity and priority defined by management. The real-time color sorting system software and hardware are briefly...

Flankers Facilitate 3-Year-Olds' Performance in a Card-Sorting Task

ERIC Educational Resources Information Center

Jordan, Patricia L.; Morton, J. Bruce

2008-01-01

Three-year-old children often act inflexibly in card-sorting tasks by continuing to sort by an old rule after being asked to switch and sort by a new rule. This inflexibility has been variously attributed to age-related constraints on higher order rule use, object redescription, and attention shifting. In 2 experiments, flankers that were…

My eSorts and Digital Extensions of Word Study

ERIC Educational Resources Information Center

Zucker, Tricia A.; Invernizzi, Marcia

2008-01-01

"My eSorts" is a strategy for helping children learn to read and spell in a socially motivated context. It is based on developmental spelling research and the word study approach to teaching phonics and spelling. "eSorting" employs digital desktop publishing tools that allow children to author their own electronic word sorts and then share these…

Skeletal Strength and Skeletogenetic Mechanisms Over Phanerozoic Time

NASA Astrophysics Data System (ADS)

Constantz, B. R.

2004-12-01

Mineralized skeletons have a remarkable range of mechanical properties with respect to strength and durability. Measurements of skeletal mechanical properties show that taxonomic groups with relatively simple, `physiochemically-dominated' modes of mineralization possess skeletal strengths and durabilities that are among the lowest of any known mineralized skeletons. Organisms with relatively sophisticated, `biologically-dominated' modes of mineralization have mechanical properties among the highest values known for any materials. These extraordinarily strong and durable skeletal materials are found in mollusks, echinoderms, vertebrates, and arthropods, which are groups with primarily mobile ecological habits. These skeletons are frequently lightweight, non-massive skeletons with little phenotypic variation. By contrast, dominant reef framework builders and reef sediment formers, with physiochemically-dominated modes of mineralization, have non-mobile ecological habits and construct massive, phenotypically plastic skeletons, possessing extremely poor mechanical properties. Endolithic organisms that further degrade the mechanical properties of the mineralized skeletons of reef builders frequently ravage their massive skeletons. As a result, the skeletons of these groups commonly fragment, and play a central role in reef establishment and maintenance, as they are incorporated in reefal, wave-resistant carbonate buildups. Scleractinian corals have a physiochemically-dominated mode of mineralization and are the dominant modern reef framework builders. Mechanical properties of modern aragonitic scleractinian coral skeletons, tested alive, demonstrate skeletal strengths that are orders of magnitude lower than those seen in mollusks, echinoderms, vertebrates, and arthropods. Rudist bivalves, the dominant reef framework-building group of the Cretaceous, show prolific, massive, highly variable, calcific skeletal elements with structures similar to some reef-forming modern, non-mobile mollusks and the skeletons of other organisms with physiochemically-dominated modes of mineralization. Many aspects of the ecological habits of reef-framework building scleractinians and rudsits are similar, including relatively high skeletal growth rates, which produce massive skeletons and wave-resistant structures with entrapped bioclastic sediments. The principal adaptive role of mineralization in reef framework building groups appears to be the rapid production of massive, brittle, wave-resistant mineralized skeletons. The physiochemically-dominated mode of mineralization of these reef framework builders appears to have made them susceptible to secular variations in Phanerozoic seawater during `calcite' and `aragonite' seas, favoring scleractinians in aragonite seas and rudists during the Cretaceous calcite episode. By contrast, most mobile mollusks, echinoderms, vertebrates, and arthropods appear relatively unaffected by secular variations in seawater chemistry over the Phanerozoic

Stratigraphy and Geochemistry of a Fond St. Jean Cinder Cone, Dominica

NASA Astrophysics Data System (ADS)

Isenburg, T.; Frey, H. M.; Waters, L. E.; Dunn, S.; Manon, M. R. F.

2017-12-01

Current geologic maps of Dominica generally classify the south-eastern portion of the island surrounding the Foundland stratovolcano as "mafic breccias and thin lava flows of Foundland center" (Smith et al. 2013). A detailed survey of the stratigraphy of a road cut at Fond St. Jean provides evidence for a mafic cinder cone on the flanks of Foundland. The 39 m thick stratigraphic sequence, dipping 60˚ north, includes a basal unit of scoria overlain by a meter of basaltic breccia and repeating layers of massive and rubbly flows, which range from 1 to 10 m in thickness. These flows transition into an additional, 2 m thick scoria deposit capped by a meter of massive basalt, which sits beneath another 3-4 m scoria deposit. Another layer of massive flow then transitions to three units of alternating air fall and ash lenses. Air fall units are 0.5 m in thickness but pinch and swell regularly, and ash lenses are roughly 10 cm thick. All units contain plagioclase + olivine + clinopyroxene + orthopyroxene + titanomagnetite. Though the phase assemblage is consistent between basaltic units, different crystal morphologies serve to define individual massive flows. Variations in the texture of materials deposited by the cinder cone provides evidence for cyclic explosive and effusive episodes. Massive samples at the bottom of the stratigraphic section contain abundant, large olivine and tabular, elongate plagioclase. Plagioclase compositions between individual stratigraphic units span a similar range in composition. Massive flows throughout the column contain similar, weakly zoned plagioclase cores (An84-94) with 10-30 µm sodic rims (An58-78; most rims are 68). Plagioclase microlites (long axes ≤100µm) span a wide range of compositions (An50-90). Three different air fall units contain plagioclase rims ranging in composition from An58-86 and cores ranging from An84-92, with the exception of a single core that has a composition of An61. Olivine in most units ranges in composition from Fo55-70. Spinels are ubiquitous throughout each of the units in the section and are consistently titanomagnetites. The potential genetic relationship between the cinder cone and Foundland is unclear, as the Foundland basalts are olivine-poor and contain amphibole, suggesting a wetter source magma for Foundland.

Ground ice formed after underground thermo-erosion of the permafrost in Alaska

NASA Astrophysics Data System (ADS)

Fortier, D.; Kanevskiy, M.; Yuri, S.

2007-12-01

Cryostratigraphic studies realized in the CRREL permafrost tunnel (¡Ö 64 57 N, 147 37 W) located near Fairbanks, Alaska revealed the presence of multi-directional reticulate ice veins and massive ice bodies in the permafrost. We propose that this reticulate-chaotic cryostructure and the massive ice bodies were formed by inward closed-system freezing of pools of water and saturated sediments trapped in underground tunnels cut in the permafrost by thermo-erosion. The massive ice and the multi-directional reticulate ice veins were likely formed after the cessation of the underground flow, either by tunnel blockage or collapse, or cessation of runoff infiltration in the permafrost. The observed tunnels were slightly inclined and could often be traced for several meters. The properties of the sediments filling these tunnels differed from the enclosing original syngenetic Pleistocene permafrost. The latter was made of ice-rich loess with abundant rootlets and was characterized by a well developed micro-lenticular cryostructure whereas the tunnels were filled with massive ice and/or organic- poor, stratified silts, sands and gravels sediments. The water content of the original syngenetic loess was about twice the water content of the sediments in the underground tunnels. The contact between the original syngenetic loess and the sediments in the tunnels was manifestly discordant and outlined by erosion lag. Release of latent heat from the poll of water and water of the saturated sediments created thaw unconformities at the tunnel boundary. Similar types of massive ice and reticulate-chaotic cryostructures were observed in Holocene to Pleistocene permafrost exposures along the Beaufort Sea Coast, on the Seward Peninsula, on the North Slope and in the Alaskan interior. The massive ice bodies and reticulate-chaotic cryostructures were always associated with, or incorporated within, ice wedges that showed signs of thermo-erosion. This indicates that the process of underground thermo-erosion has occurred widely in Alaska. On Bylot Island in the Canadian Arctic archipelago, Fortier et al. (2007) observed that extensive gullying of the permafrost resulted from the process of underground thermo-erosion. More studies are needed to determine the role of this process in the evolution of ice-wedge polygons landscape in Alaska. Fortier, D., Allard, M., Shur, Y. 2007. Observation of rapid drainage system development by thermal erosion of ice wedges on Bylot Island, Canadian Arctic Archipelago. Permafrost and Periglacial Processes 18 (3): 229-243.

Continuous sorting of Brownian particles using coupled photophoresis and asymmetric potential cycling.

PubMed

Ng, Tuck Wah; Neild, Adrian; Heeraman, Pascal

2008-03-15

Feasible sorters need to function rapidly and permit the input and delivery of particles continuously. Here, we describe a scheme that incorporates (i) restricted spatial input location and (ii) orthogonal sort and movement direction features. Sorting is achieved using an asymmetric potential that is cycled on and off, whereas movement is accomplished using photophoresis. Simulations with 0.2 and 0.5 microm diameter spherical particles indicate that sorting can commence quickly from a continuous stream. Procedures to optimize the sorting scheme are also described.

Application of Raman spectroscopy to identification and sorting of post-consumer plastics for recycling

DOEpatents

Sommer, Edward J.; Rich, John T.

2001-01-01

A high accuracy rapid system for sorting a plurality of waste products by polymer type. The invention involves the application of Raman spectroscopy and complex identification techniques to identify and sort post-consumer plastics for recycling. The invention reads information unique to the molecular structure of the materials to be sorted to identify their chemical compositions and uses rapid high volume sorting techniques to sort them into product streams at commercially viable throughput rates. The system employs a laser diode (20) for irradiating the material sample (10), a spectrograph (50) is used to determine the Raman spectrum of the material sample (10) and a microprocessor based controller (70) is employed to identify the polymer type of the material sample (10).

PTF11mnb: First analog of supernova 2005bf. Long-rising, double-peaked supernova Ic from a massive progenitor

NASA Astrophysics Data System (ADS)

Taddia, F.; Sollerman, J.; Fremling, C.; Karamehmetoglu, E.; Quimby, R. M.; Gal-Yam, A.; Yaron, O.; Kasliwal, M. M.; Kulkarni, S. R.; Nugent, P. E.; Smadja, G.; Tao, C.

2018-01-01

Aims: We study PTF11mnb, a He-poor supernova (SN) whose light curves resemble those of SN 2005bf, a peculiar double-peaked stripped-envelope (SE) SN, until the declining phase after the main peak. We investigate the mechanism powering its light curve and the nature of its progenitor star. Methods: Optical photometry and spectroscopy of PTF11mnb are presented. We compared light curves, colors and spectral properties to those of SN 2005bf and normal SE SNe. We built a bolometric light curve and modeled this light curve with the SuperNova Explosion Code (SNEC) hydrodynamical code explosion of a MESA progenitor star and semi-analytic models. Results: The light curve of PTF11mnb turns out to be similar to that of SN 2005bf until 50 d when the main (secondary) peaks occur at -18.5 mag. The early peak occurs at 20 d and is about 1.0 mag fainter. After the main peak, the decline rate of PTF11mnb is remarkably slower than what was observed in SN 2005bf, and it traces well the 56Co decay rate. The spectra of PTF11mnb reveal a SN Ic and have no traces of He unlike in the case of SN Ib 2005bf, although they have velocities comparable to those of SN 2005bf. The whole evolution of the bolometric light curve is well reproduced by the explosion of a massive (Mej = 7.8 M⊙), He-poor star characterized by a double-peaked 56Ni distribution, a total 56Ni mass of 0.59 M⊙, and an explosion energy of 2.2 × 1051 erg. Alternatively, a normal SN Ib/c explosion (M(56Ni) = 0.11 M⊙, EK = 0.2 × 1051 erg, Mej = 1 M⊙) can power the first peak while a magnetar, with a magnetic field characterized by B = 5.0 × 1014 G, and a rotation period of P = 18.1 ms, provides energy for the main peak. The early g-band light curve can be fit with a shock-breakout cooling tail or an extended envelope model from which a radius of at least 30 R⊙ is obtained. Conclusions: We presented a scenario where PTF11mnb was the explosion of a massive, He-poor star, characterized by a double-peaked 56Ni distribution. In this case, the ejecta mass and the absence of He imply a large ZAMS mass ( 85 M⊙) for the progenitor, which most likely was a Wolf-Rayet star, surrounded by an extended envelope formed either by a pre-SN eruption or due to a binary configuration. Alternatively, PTF11mnb could be powered by a SE SN with a less massive progenitor during the first peak and by a magnetar afterward. Photometric tables are only available at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/609/A106

Aflatoxin and nutrient contents of peanut collected from local market and their processed foods

NASA Astrophysics Data System (ADS)

Ginting, E.; Rahmianna, A. A.; Yusnawan, E.

2018-01-01

Peanut is succeptable to aflatoxin contamination and the sources of peanut as well as processing methods considerably affect aflatoxin content of the products. Therefore, the study on aflatoxin and nutrient contents of peanut collected from local market and their processed foods were performed. Good kernels of peanut were prepared into fried peanut, pressed-fried peanut, peanut sauce, peanut press cake, fermented peanut press cake (tempe) and fried tempe, while blended kernels (good and poor kernels) were processed into peanut sauce and tempe and poor kernels were only processed into tempe. The results showed that good and blended kernels which had high number of sound/intact kernels (82,46% and 62,09%), contained 9.8-9.9 ppb of aflatoxin B1, while slightly higher level was seen in poor kernels (12.1 ppb). However, the moisture, ash, protein, and fat contents of the kernels were similar as well as the products. Peanut tempe and fried tempe showed the highest increase in protein content, while decreased fat contents were seen in all products. The increase in aflatoxin B1 of peanut tempe prepared from poor kernels > blended kernels > good kernels. However, it averagely decreased by 61.2% after deep-fried. Excluding peanut tempe and fried tempe, aflatoxin B1 levels in all products derived from good kernels were below the permitted level (15 ppb). This suggests that sorting peanut kernels as ingredients and followed by heat processing would decrease the aflatoxin content in the products.

Unsupervised neural spike sorting for high-density microelectrode arrays with convolutive independent component analysis.

PubMed

Leibig, Christian; Wachtler, Thomas; Zeck, Günther

2016-09-15

Unsupervised identification of action potentials in multi-channel extracellular recordings, in particular from high-density microelectrode arrays with thousands of sensors, is an unresolved problem. While independent component analysis (ICA) achieves rapid unsupervised sorting, it ignores the convolutive structure of extracellular data, thus limiting the unmixing to a subset of neurons. Here we present a spike sorting algorithm based on convolutive ICA (cICA) to retrieve a larger number of accurately sorted neurons than with instantaneous ICA while accounting for signal overlaps. Spike sorting was applied to datasets with varying signal-to-noise ratios (SNR: 3-12) and 27% spike overlaps, sampled at either 11.5 or 23kHz on 4365 electrodes. We demonstrate how the instantaneity assumption in ICA-based algorithms has to be relaxed in order to improve the spike sorting performance for high-density microelectrode array recordings. Reformulating the convolutive mixture as an instantaneous mixture by modeling several delayed samples jointly is necessary to increase signal-to-noise ratio. Our results emphasize that different cICA algorithms are not equivalent. Spike sorting performance was assessed with ground-truth data generated from experimentally derived templates. The presented spike sorter was able to extract ≈90% of the true spike trains with an error rate below 2%. It was superior to two alternative (c)ICA methods (≈80% accurately sorted neurons) and comparable to a supervised sorting. Our new algorithm represents a fast solution to overcome the current bottleneck in spike sorting of large datasets generated by simultaneous recording with thousands of electrodes. Copyright © 2016 Elsevier B.V. All rights reserved.

Automatic Color Sorting of Hardwood Edge-Glued Panel Parts

Treesearch

D. Earl Kline; Richard Conners; Qiang Lu; Philip A. Araman

1997-01-01

This paper describes an automatic color sorting system for red oak edge-glued panel parts. The color sorting system simultaneously examines both faces of a panel part and then determines which face has the "best" color, and sorts the part into one of a number of color classes at plant production speeds. Initial test results show that the system generated over...

Two Types of Perseveration in the Dimension Change Card Sort Task

ERIC Educational Resources Information Center

Hanania, Rima

2010-01-01

In the Dimension Change Card Sort (DCCS) task, 3-year-olds can sort cards well by one dimension but have difficulty in switching to sort the same cards by another dimension when asked; that is, they perseverate on the first relevant information. What is the information that children perseverate on? Using a new version of the DCCS, the experiments…

Lithology and base of the surficial aquifer system, Palm Beach County, Florida

USGS Publications Warehouse

Miller, Wesley L.

1987-01-01

The surficial aquifer system is a major source of freshwater in Palm Beach County. In 1982, public supply withdrawals from the aquifer system totaled 33,543 million gallons, 77.5% of total public supply withdrawals. To evaluate the aquifer system and its geologic framework, a cooperative study with Palm Beach County was begun in 1982 by the U.S. Geological Survey. The surficial aquifer system in Palm Beach County is composed primarily of sand, sandstone, shell, silt, calcareous clay (marl), and limestone deposited during the Pleistocene and Pliocene epochs. In the western two-thirds of Palm Beach County, sediments in the aquifer system are poorly consolidated sand, shell, and sandy limestone. Owing to interspersed calcareous clays and silt and very poorly sorted materials, permeabilities in this zone of the aquifer system are relatively low. Two other zones of the aquifer system are found in the eastern one-third of the county where the sediments are appreciably more permeable than in the west due to better sorting and less silt and clay content. The location of more detailed lithologic logs for wells in these sections, along with data from nearby wells, allowed enhanced interpretation and depiction of the lithology which had previously been generalized. The most permeable zone of the aquifer system in this area is characterized by highly developed secondary porosity where infiltrating rainwater and solution by groundwater have removed calcitic-cementing materials from the sediments to produce interconnected cavities. Increased permeability in the aquifer system is generally coincident with the eastern boundary of the overlying organic soils and Lake Flirt Marl. Lithologic logs of wells in Palm Beach County indicate that sediments forming the aquifer system were deposited directly on the erosional surface of the Hawthorn Formation in some areas. In other locations in the county, lithologic logs indicate that the base of the aquifer system was formed by fluvial deposits containing erosional materials from the Tamiami and Hawthorn Formations and Caloosahatchee Marl. (Lantz-PTT)

Cell-Free Reconstitution of Multivesicular Body Formation and Receptor Sorting

PubMed Central

Sun, Wei; Vida, Thomas A.; Sirisaengtaksin, Natalie; Merrill, Samuel A.; Hanson, Phyllis I.; Bean, Andrew J.

2010-01-01

The number of surface membrane proteins and their residence time on the plasma membrane are critical determinants of cellular responses to cues that can control plasticity, growth and differentiation. After internalization, the ultimate fate of many plasma membrane proteins is dependent on whether they are sorted for internalization into the lumenal vesicles of multivesicular bodies (MVBs), an obligate step prior to lysosomal degradation. To help to elucidate the mechanisms underlying MVB sorting, we have developed a novel cell-free assay that reconstitutes the sorting of a prototypical membrane protein, the epidermal growth factor receptor, with which we have probed some of its molecular requirements. The sorting event measured is dependent on cytosol, ATP, time, temperature and an intact proton gradient. Depletion of Hrs inhibited biochemical and morphological measures of sorting that were rescued by inclusion of recombinant Hrs in the assay. Moreover, depletion of signal-transducing adaptor molecule (STAM), or addition of mutated ATPase-deficient Vps4, also inhibited sorting. This assay reconstitutes the maturation of late endosomes, including the formation of internal vesicles and the sorting of a membrane protein, and allows biochemical investigation of this process. PMID:20214752

An Unsupervised Online Spike-Sorting Framework.

PubMed

Knieling, Simeon; Sridharan, Kousik S; Belardinelli, Paolo; Naros, Georgios; Weiss, Daniel; Mormann, Florian; Gharabaghi, Alireza

2016-08-01

Extracellular neuronal microelectrode recordings can include action potentials from multiple neurons. To separate spikes from different neurons, they can be sorted according to their shape, a procedure referred to as spike-sorting. Several algorithms have been reported to solve this task. However, when clustering outcomes are unsatisfactory, most of them are difficult to adjust to achieve the desired results. We present an online spike-sorting framework that uses feature normalization and weighting to maximize the distinctiveness between different spike shapes. Furthermore, multiple criteria are applied to either facilitate or prevent cluster fusion, thereby enabling experimenters to fine-tune the sorting process. We compare our method to established unsupervised offline (Wave_Clus (WC)) and online (OSort (OS)) algorithms by examining their performance in sorting various test datasets using two different scoring systems (AMI and the Adamos metric). Furthermore, we evaluate sorting capabilities on intra-operative recordings using established quality metrics. Compared to WC and OS, our algorithm achieved comparable or higher scores on average and produced more convincing sorting results for intra-operative datasets. Thus, the presented framework is suitable for both online and offline analysis and could substantially improve the quality of microelectrode-based data evaluation for research and clinical application.

Associations between deprived life circumstances, wellbeing and self-rated health in a socially marginalized population.

PubMed

Pedersen, Pia V; Grønbæk, Morten; Curtis, Tine

2012-10-01

Previous studies of self-rated health among socially marginalized people provide insufficient understandings of what influences their self-rated health. This study aimed to examine how disadvantaged life circumstances (homelessness, substance abuse, poverty) and general well-being were associated with poor self-rated health among the socially marginalized. In a nationwide survey in Denmark, 1348 users of shelters, drop-in centres, treatment centres and social psychiatric centres answered a self-administered questionnaire. We analysed data using logistic regression. Disadvantaged life circumstances and well-being were associated with self-rated health, also when controlling for illness, mental disorder and age. Male respondents exposed to two or more disadvantaged life circumstances had higher odds of poor self-rated health [odds ratio (OR): 2.96; 95% confidence interval (CI): 1.80-4.87] than males exposed to fewer disadvantages. A low sense of personal well-being implied higher odds of poor self-rated health among both men and women. Among men, not showering regularly (OR: 1.81; 95% CI: 1.17-2.79), and among women, not eating varied food (OR: 2.24; 95% CI: 1.20-4.20) and exposure to physical violence (borderline significant) implied higher odds of poor self-rated health. Male and female respondents reporting lack of sleep and loneliness (borderline significant among women) had higher odds of poor self-rated health. The poor self-rated health among socially marginalized is strongly associated with massive social problems, poor living conditions and poor well-being. This study elucidates the need for more broadly based and holistic initiatives by both the health sector and the social services, incorporating health promotion initiatives into social work.

Design of mechanical arm for an automatic sorting system of recyclable cans

NASA Astrophysics Data System (ADS)

Resti, Y.; Mohruni, A. S.; Burlian, F.; Yani, I.; Amran, A.

2018-04-01

The use of a mechanical arm for an automatic sorting system of used cans should be designed carefully. The right design will result in a high precision sorting rate and a short sorting time. The design includes first; design manipulator,second; determine link and joint specifications, and third; build mechanical systems and control systems. This study aims to design the mechanical arm as a hardware system for automatic cans sorting system. The material used for the manipulator is the aluminum plate. The manipulator is designed using 6 links and 6 join where the 6th link is the end effectorand the 6th join is the gripper. As a driving motor used servo motor, while as a microcontroller used Arduino Uno which is connected with Matlab programming language. Based on testing, a mechanical arm designed for this recyclable canned recycling system has a precision sorting rate at 93%, where the average total time required for sorting is 10.82 seconds.

Low power and high accuracy spike sorting microprocessor with on-line interpolation and re-alignment in 90 nm CMOS process.

PubMed

Chen, Tung-Chien; Ma, Tsung-Chuan; Chen, Yun-Yu; Chen, Liang-Gee

2012-01-01

Accurate spike sorting is an important issue for neuroscientific and neuroprosthetic applications. The sorting of spikes depends on the features extracted from the neural waveforms, and a better sorting performance usually comes with a higher sampling rate (SR). However for the long duration experiments on free-moving subjects, the miniaturized and wireless neural recording ICs are the current trend, and the compromise on sorting accuracy is usually made by a lower SR for the lower power consumption. In this paper, we implement an on-chip spike sorting processor with integrated interpolation hardware in order to improve the performance in terms of power versus accuracy. According to the fabrication results in 90nm process, if the interpolation is appropriately performed during the spike sorting, the system operated at the SR of 12.5 k samples per second (sps) can outperform the one not having interpolation at 25 ksps on both accuracy and power.

Arroyo el Mimbral, Mexico, K/T unit: Origin as debris flow/turbidite, not a tsunami deposit

NASA Technical Reports Server (NTRS)

Bohor, Bruce F.; Betterton, William J.

1993-01-01

Coarse, spherule-bearing, elastic units have been discovered at 10 marine sites that span the K/T boundary in northeastern Mexico. We examined one of the best exposed sites in Arroyo el Mimbral, northwest of Tampico. The Mimbral outcrop displays a layered elastic unit up to 3 m thick enclosed by marly limestones of the Mendez (Latest Maastrichian) and Velasco (Earliest Danian) Formations. At its thickest point, this channelized elastic unit is comprised of 3 subunits: (1) a basal, poorly-sorted, ungraded calcareous spherule bed 1 m thick containing relict impact glass and shocked mineral grains, (2) a massive set of laminated calcite-cemented sandstones up to 2 m thick with plant debris at its base, (3) capped by a thin (up to 20 cm) set of rippled sandstone layers separated by silty mudstone drapes containing a small (921 pg/g) iridium anomaly. This tripartite elastic unit is conformably overlain by marls of the Velasco Formation. We also visited the La Lajilla site east of Ciudad Victoria; its stratigraphy is similar to Mimbral's, but its elastic beds are thinner and less extensive laterally. The Mimbral elastic unit has been interpreted previously as being deposited by a megawave or tsunami produced by an asteroid impact on nearby Yucatan (Chicxulub crater). However, a presumed 400-m paleodepth of water at the Mimbral site, channeling of the spherule subunit into the underlying Mendez Formation marls, and the overtopping of the basal, spherule-bearing subunit by the laminated sandstone subunit, all suggest a combined debris flow/turbidite origin for this elastic unit similar to that proposed for Upper Pleistocene sand/silt beds occurring elsewhere in the Gulf of Mexico. In this latter model, the sediment source region for the elastic unit is the lower continental shelf and slope escarpment. For the K/T unit at Mimbral, we propose that thick ejecta blanket deposits composed mostly of spherules were rapidly loaded onto the lower shelf and slope from an impact-generated ejecta curtain.

Detrital cave sediments record Late Quaternary hydrologic and climatic variability in northwestern Florida, USA

NASA Astrophysics Data System (ADS)

Winkler, Tyler S.; van Hengstum, Peter J.; Horgan, Meghan C.; Donnelly, Jeffrey P.; Reibenspies, Joseph H.

2016-04-01

Detrital sediment in Florida's (USA) submerged cave systems may preserve records of regional climate and hydrologic variability. However, the basic sedimentology, mineralogy, stratigraphic variability, and emplacement history of the successions in Florida's submerged caves remains poorly understood. Here we present stratigraphic, mineralogical, and elemental data on sediment cores from two phreatic cave systems in northwestern Florida (USA), on the Dougherty Karst Plain: Hole in the Wall Cave (HITW) and Twin Cave. Water flowing through these caves is subsurface flow in the Apalachicola River drainage basin, and the caves are located just downstream from Jackson Blue (1st magnitude spring, > 2.8 m3 s- 1 discharge). Sedimentation in these caves is dominated by three primary sedimentary styles: (i) ferromanganese deposits dominate the basal recovered stratigraphy, which pass upsection into (ii) poorly sorted carbonate sediment, and finally into (iii) fine-grained organic matter (gyttja) deposits. Resolving the emplacement history of the lower stratigraphic units was hampered by a lack of suitable material for radiocarbon dating, but the upper organic-rich deposits have a punctuated depositional history beginning in the earliest Holocene. For example, gyttja primarily accumulated in HITW and Twin Caves from ~ 5500 to 3500 cal yr. BP, which coincides with regional evidence for water-table rise of the Upper Floridian Aquifer associated with relative sea-level rise in the Gulf of Mexico, and evidence for invigorated drainage through the Apalachicola River drainage basin. Gyttja sediments were also deposited in one of the caves during the Bølling/Allerød climate oscillation. Biologically, these results indicate that some Floridian aquatic cave (stygobitic) ecosystems presently receive minimal organic matter supply in comparison to prehistoric intervals. The pre-Holocene poorly sorted carbonate sediment contains abundant invertebrate fossils, and likely documents a period of enhanced limestone dissolution and cave formation (speleogenesis) during lower paleo water levels. Further work is still required to (a) determine whether precipitation of the ferromanganese deposits is inorganically or biologically mediated, (b) temporally constrain the emplacement history of the primary sedimentary styles, and (c) determine the full geographic extent of these sedimentary signals. However, these preliminary observations suggest that sedimentation in the inland underwater caves of northwestern Florida is related to Quaternary-scale hydrographic variability in the Apalachicola River drainage basin in response to broader ocean and atmospheric forcing.

Algorithm Sorts Groups Of Data

NASA Technical Reports Server (NTRS)

Evans, J. D.

1987-01-01

For efficient sorting, algorithm finds set containing minimum or maximum most significant data. Sets of data sorted as desired. Sorting process simplified by reduction of each multielement set of data to single representative number. First, each set of data expressed as polynomial with suitably chosen base, using elements of set as coefficients. Most significant element placed in term containing largest exponent. Base selected by examining range in value of data elements. Resulting series summed to yield single representative number. Numbers easily sorted, and each such number converted back to original set of data by successive division. Program written in BASIC.

Mathematical modelling of the transport of a poorly sorted granular mixture as a debris-flow. The case of Madeira Island torrential floods in 2010

NASA Astrophysics Data System (ADS)

Ferreira, Rui M. L.; Oliveira, Rodrigo P.; Conde, Daniel

2016-04-01

On the 20th February 2010, heavy rainfall was registered at Madeira Island, North Atlantic. Stony debris flows, mudflows and mudslides ensued causing severe property loss, 1.5 m thick sediment deposits at downtown Funchal including 16th century monuments, and a death toll of 47 lives. Debris-flow fronts propagated downstream while carrying very high concentrations of solid material. These two-phase solid-fluid flows were responsible for most of the infrastructural damage across the island, due to their significantly increased mass and momentum. The objective of the present modelling work is to validate a 2DH model for torrential flows featuring the transport and interaction of several size fractions of a poorly-sorted granular mixture typical of stony debris flow in Madeira. The module for the transport of poorly-sorted material was included in STAV-2D (CERIS-IST), a shallow-water and morphology solver based on a finite-volume method using a flux-splitting technique featuring a reviewed Roe-Riemann solver, with appropriate source-term formulations to ensure full conservativeness. STAV-2D also includes formulations of flow resistance and bedload transport adequate for debris-flows with natural mobile beds (Ferreira et al., 2009) and has been validated with both theoretical solutions and laboratory data (Soares-Frazão et al., 2012; Canelas et al., 2013). The modelling of the existing natural and built environment is fully explicit. All buildings, streets and channels are accurately represented within the mesh geometry. Such detail is relevant for the reliability of the validation using field data, since the major sedimentary deposits within the urban meshwork of Funchal were identified and characterized in terms of volume and grain size distribution during the aftermath of the 20th February of 2010 event. Indeed, the measure of the quality of the numerical results is the agreement between simulated and estimated volume of deposited sediment and between estimated and modelled grain-size distribution of the deposits. The formulations expressing closures for size fraction interaction and active layer dynamics are discussed. The simulation tool resulting from this modelling effort is expected to help the establishment of new methodologies and parameters for hydraulic design and hazard assessment in the Island of Madeira. Acknowledgements This work was partially funded by FEDER, program COMPETE, and by national funds through Portuguese Foundation for Science and Technology (FCT) project RECI/ECM-HID/0371/2012. References Canelas, R.; Murillo, J. & Ferreira, R.M.L. (2013). Two-dimensional depth-averaged modelling of dambreak flows over mobile beds. Journal of Hydraulic Research, 51(4), 392-407. Ferreira, R. M. L., Franca, M. J., Leal, J. G., & Cardoso, A. H. (2009). Mathematical modelling of shallow flows: Closure models drawn from grain-scale mechanics of sediment transport and flow hydrodynamics. Canadian Journal of Civil Engineering, 36(10), 1605-1621. Soares-Frazão and IAHR Working group for Dam-break flows over mobile beds (2012) Dam-break flows over mobile beds: Experiments and benchmark tests for numerical models. Journal of Hydraulic Research, 50(4), 364-375. DOI: 10.1080/00221686.2012.689682

Chip-based droplet sorting

DOEpatents

Beer, Neil Reginald; Lee, Abraham; Hatch, Andrew

2014-07-01

A non-contact system for sorting monodisperse water-in-oil emulsion droplets in a microfluidic device based on the droplet's contents and their interaction with an applied electromagnetic field or by identification and sorting.

A QR code identification technology in package auto-sorting system

NASA Astrophysics Data System (ADS)

di, Yi-Juan; Shi, Jian-Ping; Mao, Guo-Yong

2017-07-01

Traditional manual sorting operation is not suitable for the development of Chinese logistics. For better sorting packages, a QR code recognition technology is proposed to identify the QR code label on the packages in package auto-sorting system. The experimental results compared with other algorithms in literatures demonstrate that the proposed method is valid and its performance is superior to other algorithms.

When Seeing Is Knowing: The Role of Visual Cues in the Dissociation between Children's Rule Knowledge and Rule Use

ERIC Educational Resources Information Center

Buss, Aaron T.; Spencer, John P.

2012-01-01

The Dimensional Change Card Sort (DCCS) task requires children to switch from sorting cards based on shape or color to sorting based on the other dimension. Typically, 3-year-olds perseverate, whereas 4-year-olds flexibly sort by different dimensions. Zelazo and colleagues (1996, Cognitive Development, 11, 37-63) asked children questions about the…

Technology to sort lumber by color and grain for furniture parts

Treesearch

D. Earl Kline; Richard Conners; Philip A. Araman

2000-01-01

This paper describes an automatic color and grain sorting system for wood edge-glued panel parts. The color sorting system simultaneously examines both faces of a panel part and then determines which face has the "best" color, and sorts the part into one of a number of color classes at plant production speeds. In-plant test results show that the system...

Bottom sediments and nutrients in the tidal Potomac system, Maryland and Virginia

USGS Publications Warehouse

Glenn, Jerry L.

1988-01-01

The characteristics and distributions of near-surface bottom sediments and of nutrients in the sediments provide information on modern sediment and nutrient sources, sedimentation environments, and geochemical reactions in the tidal Potomac system, Maryland and Virginia. This information is fundamental to an improved understanding of sedimentation and eutrophication problems in the tidal Potomac system. The tidal Potomac system consists of 1,230 square kilometers of intertidal to subtidal Potomac mainstem and tributary streambed from the heads-of-tides to Chesapeake Bay. Tidal Potomac sediments are dominantly silt and clay except in local areas. An average sediment sample is about two-thirds silt and clay (fine) particles and one-third sand (coarse) particles. The mean of the median size of all samples is 6.60 phi, or 0.010 millimeters. Sorting generally is poor and the average sediment is skewed toward the fine tail of the size-distribution curve. Mean particle-size measures have large standard deviations. Among geomorphic units, two distinctly different size populations are found; fine (median phi about 9), and poorly sorted (sorting about 3) sediments in the channel and the smooth flat, and coarse (median phi about 2), and well sorted (sorting about 1) sediments in the shoreline flat and the irregular slope. Among mainstem hydrologic divisions, an average sediment from the river and the estuary division is coarser and more variable than an average sediment from the transition division. Substantial concentrations of total carbon, total nitrogen, and total phosphorus, and limited amounts of inorganic carbon, ammonia nitrogen and nitrite plus nitrate nitrogen occur in tidal Potomac sediments. An average tidal Potomac sediment sample weighing 1 kilogram contains about 21,000 milligrams of total carbon, 2,400 milligrams of total nitrogen, 1,200 milligrams of total phosphorus, 600 milligrams of inorganic carbon, 170 milligrams of ammonia nitrogen, and 2 milligrams of nitrite plus nitrate nitrogen. Total carbon, nitrogen, and phosphorus have an average ratio by weight of 18:2:1 and an average ratio by atoms of 94:8:1. Nutrient concentrations and nutrient ratios have large ranges and standard deviations. Nutrient concentrations usually are closely related to particle size; large concentrations are characteristic of fine sediments in the channel and the smooth flat, and small concentrations are typical of coarse sediments in the shoreline flat and the irregular slope. Concentrations typically decrease from the river division to the estuary division. Mainstem and tributaries show no statistically significant difference in mean particle-size measures or mean nutrient concentrations. Tributaries do not contribute large quantities of sediment with diverse texture or nutrient content to the Potomac mainstem. Particle-size measures and nutrient concentrations in the mainstem are significantly related to hydrologic divisions and geomorphic units; that is, particle size and nutrients vary significantly along and across the Potomac mainstem. Lateral variations in particle size and nutrient content are more pronounced and contribute more to significant relations than longitudinal variations contribute. The mean values for the median particle size and for the percentage of sand indicate significant variations among hydrologic divisions for samples from a geomorphic unit, and among geomorphic units, for samples from a hydrologic division. Sediments of channels and smooth flats in the river division commonly are coarser than sediments of channels and smooth flats in the transition and the estuary divisions. Shoreline flats in the estuary division are coarser than shoreline flats in the river division. Shoreline flats and irregular slopes in each hydrologic division generally are significantly coarser than channels and smooth flats. Relations between particle-size measures and geomorphic units show progressively larger cor

BayesMotif: de novo protein sorting motif discovery from impure datasets.

PubMed

Hu, Jianjun; Zhang, Fan

2010-01-18

Protein sorting is the process that newly synthesized proteins are transported to their target locations within or outside of the cell. This process is precisely regulated by protein sorting signals in different forms. A major category of sorting signals are amino acid sub-sequences usually located at the N-terminals or C-terminals of protein sequences. Genome-wide experimental identification of protein sorting signals is extremely time-consuming and costly. Effective computational algorithms for de novo discovery of protein sorting signals is needed to improve the understanding of protein sorting mechanisms. We formulated the protein sorting motif discovery problem as a classification problem and proposed a Bayesian classifier based algorithm (BayesMotif) for de novo identification of a common type of protein sorting motifs in which a highly conserved anchor is present along with a less conserved motif regions. A false positive removal procedure is developed to iteratively remove sequences that are unlikely to contain true motifs so that the algorithm can identify motifs from impure input sequences. Experiments on both implanted motif datasets and real-world datasets showed that the enhanced BayesMotif algorithm can identify anchored sorting motifs from pure or impure protein sequence dataset. It also shows that the false positive removal procedure can help to identify true motifs even when there is only 20% of the input sequences containing true motif instances. We proposed BayesMotif, a novel Bayesian classification based algorithm for de novo discovery of a special category of anchored protein sorting motifs from impure datasets. Compared to conventional motif discovery algorithms such as MEME, our algorithm can find less-conserved motifs with short highly conserved anchors. Our algorithm also has the advantage of easy incorporation of additional meta-sequence features such as hydrophobicity or charge of the motifs which may help to overcome the limitations of PWM (position weight matrix) motif model.

Effects of Diclofenac, L-NAME, L-Arginine, and Pentadecapeptide BPC 157 on Gastrointestinal, Liver, and Brain Lesions, Failed Anastomosis, and Intestinal Adaptation Deterioration in 24 Hour-Short-Bowel Rats.

PubMed

Lojo, Nermin; Rasic, Zarko; Zenko Sever, Anita; Kolenc, Danijela; Vukusic, Darko; Drmic, Domagoj; Zoricic, Ivan; Sever, Marko; Seiwerth, Sven; Sikiric, Predrag

2016-01-01

Stable gastric pentadecapeptide BPC 157 was previously used to ameliorate wound healing following major surgery and counteract diclofenac toxicity. To resolve the increasing early risks following major massive small bowel resectioning surgery, diclofenac combined with nitric oxide (NO) system blockade was used, suggesting therapy with BPC 157 and the nitric oxide synthase (NOS substrate) L-arginine, is efficacious. Immediately after anastomosis creation, short-bowel rats were untreated or administered intraperitoneal diclofenac (12 mg/kg), BPC 157 (10 μg/kg or 10 ng/kg), L-NG-nitroarginine methyl ester (L-NAME, 5 mg/kg), L-arginine (100 mg/kg) alone or combined, and assessed 24 h later. Short-bowel rats exhibited poor anastomosis healing, failed intestine adaptation, and gastrointestinal, liver, and brain lesions, which worsened with diclofenac. This was gradually ameliorated by immediate therapy with BPC 157 and L-arginine. Contrastingly, NOS-blocker L-NAME induced further aggravation and lesions gradually worsened. Specifically, rats with surgery alone exhibited mild stomach/duodenum lesions, considerable liver lesions, and severe cerebral/hippocampal lesions while those also administered diclofenac showed widespread severe lesions in the gastrointestinal tract, liver, cerebellar nuclear/Purkinje cells, and cerebrum/hippocampus. Rats subjected to surgery, diclofenac, and L-NAME exhibited the mentioned lesions, worsening anastomosis, and macro/microscopical necrosis. Thus, rats subjected to surgery alone showed evidence of deterioration. Furtheremore, rats subjected to surgery and administered diclofenac showed worse symptoms, than the rats subjected to surgery alone did. Rats subjected to surgery combined with diclofenac and L-NAME showed the worst deterioration. Rats subjected to surgery exhibited habitual adaptation of the remaining small intestine, which was markedly reversed in rats subjected to surgery and diclofenac, and those with surgery, diclofenac, and L-NAME. BPC 157 completely ameliorated symptoms in massive intestinal resection-, massive intestinal resection plus diclofenac-, and massive intestinal resection plus diclofenac plus L-NAME-treated short bowel rats that presented with cyclooxygenase (COX)-NO-system inhibition. L-arginine ameliorated only L-NAME-induced aggravation of symptoms in rats subjected to massive intestinal resection and administered diclofenac plus L-NAME.

Effects of Diclofenac, L-NAME, L-Arginine, and Pentadecapeptide BPC 157 on Gastrointestinal, Liver, and Brain Lesions, Failed Anastomosis, and Intestinal Adaptation Deterioration in 24 Hour-Short-Bowel Rats

PubMed Central

Lojo, Nermin; Rasic, Zarko; Zenko Sever, Anita; Kolenc, Danijela; Vukusic, Darko; Drmic, Domagoj; Zoricic, Ivan; Sever, Marko; Seiwerth, Sven; Sikiric, Predrag

2016-01-01

Stable gastric pentadecapeptide BPC 157 was previously used to ameliorate wound healing following major surgery and counteract diclofenac toxicity. To resolve the increasing early risks following major massive small bowel resectioning surgery, diclofenac combined with nitric oxide (NO) system blockade was used, suggesting therapy with BPC 157 and the nitric oxide synthase (NOS substrate) L-arginine, is efficacious. Immediately after anastomosis creation, short-bowel rats were untreated or administered intraperitoneal diclofenac (12 mg/kg), BPC 157 (10 μg/kg or 10 ng/kg), L-NG-nitroarginine methyl ester (L-NAME, 5 mg/kg), L-arginine (100 mg/kg) alone or combined, and assessed 24 h later. Short-bowel rats exhibited poor anastomosis healing, failed intestine adaptation, and gastrointestinal, liver, and brain lesions, which worsened with diclofenac. This was gradually ameliorated by immediate therapy with BPC 157 and L-arginine. Contrastingly, NOS-blocker L-NAME induced further aggravation and lesions gradually worsened. Specifically, rats with surgery alone exhibited mild stomach/duodenum lesions, considerable liver lesions, and severe cerebral/hippocampal lesions while those also administered diclofenac showed widespread severe lesions in the gastrointestinal tract, liver, cerebellar nuclear/Purkinje cells, and cerebrum/hippocampus. Rats subjected to surgery, diclofenac, and L-NAME exhibited the mentioned lesions, worsening anastomosis, and macro/microscopical necrosis. Thus, rats subjected to surgery alone showed evidence of deterioration. Furtheremore, rats subjected to surgery and administered diclofenac showed worse symptoms, than the rats subjected to surgery alone did. Rats subjected to surgery combined with diclofenac and L-NAME showed the worst deterioration. Rats subjected to surgery exhibited habitual adaptation of the remaining small intestine, which was markedly reversed in rats subjected to surgery and diclofenac, and those with surgery, diclofenac, and L-NAME. BPC 157 completely ameliorated symptoms in massive intestinal resection-, massive intestinal resection plus diclofenac-, and massive intestinal resection plus diclofenac plus L-NAME-treated short bowel rats that presented with cyclooxygenase (COX)-NO-system inhibition. L-arginine ameliorated only L-NAME-induced aggravation of symptoms in rats subjected to massive intestinal resection and administered diclofenac plus L-NAME. PMID:27627764

Massive pre-main-sequence stars in M17

NASA Astrophysics Data System (ADS)

Ramírez-Tannus, M. C.; Kaper, L.; de Koter, A.; Tramper, F.; Bik, A.; Ellerbroek, L. E.; Ochsendorf, B. B.; Ramírez-Agudelo, O. H.; Sana, H.

2017-08-01

The formation process of massive stars is still poorly understood. Massive young stellar objects (mYSOs) are deeply embedded in their parental clouds; these objects are rare, and thus typically distant, and their reddened spectra usually preclude the determination of their photospheric parameters. M17 is one of the best-studied H II regions in the sky, is relatively nearby, and hosts a young stellar population. We have obtained optical to near-infrared spectra of previously identified candidate mYSOs and a few OB stars in this region with X-shooter on the ESO Very Large Telescope. The large wavelength coverage enables a detailed spectroscopic analysis of the photospheres and circumstellar disks of these candidate mYSOs. We confirm the pre-main-sequence (PMS) nature of six of the stars and characterise the O stars. The PMS stars have radii that are consistent with being contracting towards the main sequence and are surrounded by a remnant accretion disk. The observed infrared excess and the double-peaked emission lines provide an opportunity to measure structured velocity profiles in the disks. We compare the observed properties of this unique sample of young massive stars with evolutionary tracks of massive protostars and propose that these mYSOs near the western edge of the H II region are on their way to become main-sequence stars ( 6-20 M⊙) after having undergone high mass accretion rates (Ṁacc 10-4-10-3M⊙yr-1). Their spin distribution upon arrival at the zero age main-sequence is consistent with that observed for young B stars, assuming conservation of angular momentum and homologous contraction. Based on observations collected at the European Southern Observatory at Paranal, Chile (ESO programmes 60.A-9404(A), 085.D-0741, 089.C-0874(A), and 091.C-0934(B)).The full normalised X-shooter spectra are available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/604/A78

The Multifactorial Epidemiology of Blackwater Fever.

PubMed

Shanks, G Dennis

2017-12-01

Blackwater fever is a massive hemolytic event usually occurring in the context of repeated falciparum malaria infections and intermittent quinine use. Its etiology is poorly understood, and it is rarely seen today. Historical epidemiological observations from the 20th century demonstrated variable patterns in prisoners in Andaman Islands, refugees in Macedonia, canal workers in Panama, expatriates in Rhodesia, and Second World War soldiers. Rates of blackwater fever per 1,000 malaria cases varied over two orders of magnitude. Islands, such as the Andaman Islands and New Guinea, had lower blackwater fever rates than continental areas. During the Second World War, blackwater fever rates in British soldiers in West Africa and Australian soldiers in New Guinea differed by a factor of 40 despite similar treatment regimens and falciparum malaria transmission risks. Blackwater fever is a complex interaction between host erythrocyte, falciparum malaria, and antimalarial drugs which remains poorly understood.

Enhanced Mobility in Concentrated Pyroclastic Density Currents: An Examination of a Self-Fluidization Mechanism

NASA Astrophysics Data System (ADS)

Breard, Eric C. P.; Dufek, Josef; Lube, Gert

2018-01-01

Pyroclastic density currents (PDCs) are a significant volcanic hazard. However, their dominant transport mechanisms remain poorly understood, in part because of the large variability of PDC types and deposits. Here we combine field data with experimental and numerical simulations to illuminate the twofold fate of particles settling from an ash cloud to form the dense PDC basal flow. At solid fractions >1 vol %, heterogeneous drag leads to formation of mesoscale particle clusters that favor rapid particle settling and result in a mobile dense layer with significant bed weight support. Conversely, at lower concentrations the absence of particle clusters typically leads to formation of poorly mobile dense beds that deposit massive layers. Based on this transport dichotomy, we present a numerical dense-dilute parameter that allows a PDC's dominant transport mechanism to be determined directly from the deposit geometry and grainsize characteristics.

Issues engulfed Saudi Arabia construction workers

NASA Astrophysics Data System (ADS)

Al-Emad, N. H.; Rahman, I. A.

2018-04-01

This paper presents an exploratory study conducted in Makkah city to uncover issues faced by construction workers from the construction leaders’ perspective. Eleven construction leaders/experts were interviewed to unleash their experiences on handling the foreign workers working in Makkah construction projects. Most of the experts are senior management staffs with more than 10 years’ working experience in Saudi Arabia construction industry. The interviews were carried out in semi structured mode where all the information was captured manually and also electronically. The identified issues were sorted based on its commonality into 10 clusters. Hence in each cluster, the numbers of issue considered by the experts are reflecting the importance of that particular cluster. The result of the clusters according to the number of issues mentioned by the experts are safety issues, restricted government regulation, demotivated issues, lack of quality workers, poor living quality, communication barriers, adaption issues, poor attitudes, lack of logistical arrangements and lack of education. With these identified issues it will assist the construction players in the construction industry of Saudi Arabia in dealing with their workers.

CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer

PubMed Central

Imai, Takayuki; Tamai, Keiichi; Oizumi, Sayuri; Oyama, Kyoko; Yamaguchi, Kazunori; Sato, Ikuro; Satoh, Kennichi; Matsuura, Kazuto; Saijo, Shigeru; Sugamura, Kazuo; Tanaka, Nobuyuki

2013-01-01

Cancer stem cells contribute to the malignant phenotypes of a variety of cancers, but markers to identify human hypopharyngeal cancer (HPC) stem cells remain poorly understood. Here, we report that the CD271+ population sorted from xenotransplanted HPCs possesses an enhanced tumor-initiating capability in immunodeficient mice. Tumors generated from the CD271+ cells contained both CD271+ and CD271− cells, indicating that the population could undergo differentiation. Immunohistological analyses of the tumors revealed that the CD271+ cells localized to a perivascular niche near CD34+ vasculature, to invasive fronts, and to the basal layer. In accordance with these characteristics, a stemness marker, Nanog, and matrix metalloproteinases (MMPs), which are implicated in cancer invasion, were significantly up-regulated in the CD271+ compared to the CD271− cell population. Furthermore, using primary HPC specimens, we demonstrated that high CD271 expression was correlated with a poor prognosis for patients. Taken together, our findings indicate that CD271 is a novel marker for HPC stem-like cells and for HPC prognosis. PMID:23626764

The Evolution of Low-Metallicity Massive Stars

NASA Astrophysics Data System (ADS)

Szécsi, Dorottya

2016-07-01

Massive star evolution taking place in astrophysical environments consisting almost entirely of hydrogen and helium - in other words, low-metallicity environments - is responsible for some of the most intriguing and energetic cosmic phenomena, including supernovae, gamma-ray bursts and gravitational waves. This thesis aims to investigate the life and death of metal-poor massive stars, using theoretical simulations of the stellar structure and evolution. Evolutionary models of rotating, massive stars (9-600 Msun) with an initial metal composition appropriate for the low-metallicity dwarf galaxy I Zwicky 18 are presented and analyzed. We find that the fast rotating models (300 km/s) become a particular type of objects predicted only at low-metallicity: the so-called Transparent Wind Ultraviolet INtense (TWUIN) stars. TWUIN stars are fast rotating massive stars that are extremely hot (90 kK), very bright and as compact as Wolf-Rayet stars. However, as opposed to Wolf-Rayet stars, their stellar winds are optically thin. As these hot objects emit intense UV radiation, we show that they can explain the unusually high number of ionizing photons of the dwarf galaxy I Zwicky 18, an observational quantity that cannot be understood solely based on the normal stellar population of this galaxy. On the other hand, we find that the most massive, slowly rotating models become another special type of object predicted only at low-metallicity: core-hydrogen-burning cool supergiant stars. Having a slow but strong stellar wind, these supergiants may be important contributors in the chemical evolution of young galactic globular clusters. In particular, we suggest that the low mass stars observed today could form in a dense, massive and cool shell around these, now dead, supergiants. This scenario is shown to explain the anomalous surface abundances observed in these low mass stars, since the shell itself, having been made of the mass ejected by the supergiant’s wind, contains nuclear burning products in the same ratio as observed today in globular clusters stars. Further elaborating the fast rotating TWUIN star models, we predict that some of them will become Wolf-Rayet stars near the end of their lives. From this we show that our models can self-consistently explain both the high ionizing flux and the number of Wolf-Rayet stars in I Zwicky 18. Moreover, some of our models are predicted to explode as long-duration gamma-ray bursts. Thus, we speculate that the high ionizing flux observed can be a signpost for upcoming gamma-ray bursts in dwarf galaxies. Although our models have been applied to interpret observations of globular clusters and dwarf galaxies, we point out that they could also be used in the context of other low-metallicity environments as well. Understanding the early Universe, for example, requires to have a solid knowledge of how massive stars at low-metallicity live and interact with their environments. Thus, we expect that the models and results presented in this thesis will be beneficial for not only the massive star community, but for the broader astronomy and cosmology community as well.

The luminous, massive and solar metallicity galaxy hosting the Swift γ-ray burst GRB 160804A at z = 0.737

NASA Astrophysics Data System (ADS)

Heintz, K. E.; Malesani, D.; Wiersema, K.; Jakobsson, P.; Fynbo, J. P. U.; Savaglio, S.; Cano, Z.; Covino, S.; D'Elia, V.; Gomboc, A.; Hammer, F.; Kaper, L.; Milvang-Jensen, B.; Møller, P.; Piranomonte, S.; Selsing, J.; Rhodin, N. H. P.; Tanvir, N. R.; Thöne, C. C.; de Ugarte Postigo, A.; Vergani, S. D.; Watson, D.

2018-02-01

We here present the spectroscopic follow-up observations with VLT/X-shooter of the Swift long-duration gamma-ray burst GRB 160804A at z = 0.737. Typically, GRBs are found in low-mass, metal-poor galaxies that constitute the sub-luminous population of star-forming galaxies. For the host galaxy of the GRB presented here, we derive a stellar mass of log (M*/ M⊙) = 9.80 ± 0.07, a roughly solar metallicity (12 + log (O/H) = 8.74 ± 0.12) based on emission line diagnostics, and an infrared luminosity of M3.6/(1 + z) = -21.94 mag, but find it to be dust-poor (E(B - V) < 0.05 mag). This establishes the galaxy hosting GRB 160804A as one of the most luminous, massive and metal-rich GRB hosts at z < 1.5. Furthermore, the gas-phase metallicity is found to be representative of the physical conditions of the gas close to the explosion site of the burst. The high metallicity of the host galaxy is also observed in absorption, where we detect several strong Fe II transitions as well as Mg II and Mg I. Although host galaxy absorption features are common in GRB afterglow spectra, we detect absorption from strong metal lines directly in the host continuum (at a time when the afterglow was contributing to < 15 per cent). Finally, we discuss the possibility that the geometry and state of the absorbing and emitting gas are indicative of a galactic scale outflow expelled at the final stage of two merging galaxies.

Investigating the diversity of supernovae type Iax: a MUSE and NOT spectroscopic study of their environments

NASA Astrophysics Data System (ADS)

Lyman, J. D.; Taddia, F.; Stritzinger, M. D.; Galbany, L.; Leloudas, G.; Anderson, J. P.; Eldridge, J. J.; James, P. A.; Krühler, T.; Levan, A. J.; Pignata, G.; Stanway, E. R.

2018-01-01

SN 2002cx-like Type Ia supernovae (also known as SNe Iax) represent one of the most numerous peculiar SN classes. They differ from normal SNe Ia by having fainter peak magnitudes, faster decline rates and lower photospheric velocities, displaying a wide diversity in these properties. We present both integral-field and long-slit visual-wavelength spectroscopy of the host galaxies and explosion sites of SNe Iax to provide constraints on their progenitor formation scenarios. The SN Iax explosion-site metallicity distribution is similar to that of core-collapse SNe and metal poor compared to either normal SNe Ia or SN 1991T-like events. Fainter members, speculated to form distinctly from brighter SN Iax, are found at a range of metallicities, extending to very metal poor environments. Although the SN Iax explosion-sites' ages and star formation rates are comparatively older and less intense than the distribution of star-forming regions across their host galaxies, we confirm the presence of young stellar populations (SPs) at explosion environments for most SNe Iax, expanded here to a larger sample. Ages of the young SPs (several × 107 to 108 yr) are consistent with predictions for young thermonuclear and electron-capture SN progenitors. The lack of extremely young SPs at the explosion sites disfavours very massive progenitors such as Wolf-Rayet explosions with significant fallback. We find weak ionized gas in the only SN Iax host without obvious signs of star formation. The source of the ionization remains ambiguous but appears unlikely to be mainly due to young, massive stars.

NGC 6273: Towards Defining A New Class of Galactic Globular Clusters?

NASA Astrophysics Data System (ADS)

Johnson, Christian I.; Rich, Robert Michael; Pilachowski, Catherine A.; Caldwell, Nelson; Mateo, Mario L.; Ira Bailey, John; Crane, Jeffrey D.

2016-01-01

A growing number of observations have found that several Galactic globular clusters exhibit abundance dispersions beyond the well-known light element (anti-)correlations. These clusters tend to be very massive, have >0.1 dex intrinsic metallicity dispersions, have complex sub-giant branch morphologies, and have correlated [Fe/H] and s-process element enhancements. Interestingly, nearly all of these clusters discovered so far have [Fe/H]~-1.7. In this context, we have examined the chemical composition of 18 red giant branch (RGB) stars in the massive, metal-poor Galactic bulge globular cluster NGC 6273 using high signal-to-noise, high resolution (R~27,000) spectra obtained with the Michigan/Magellan Fiber System (M2FS) and MSpec spectrograph mounted on the Magellan-Clay 6.5m telescope at Las Campanas Observatory. We find that the cluster exhibits a metallicity range from [Fe/H]=-1.80 to -1.30 and is composed of two dominant populations separated in [Fe/H] and [La/Fe] abundance. The increase in [La/Eu] as a function of [La/H] suggests that the increase in [La/Fe] with [Fe/H] is due to almost pure s-process enrichment. The most metal-rich star in our sample is not strongly La-enhanced, but is α-poor and may belong to a third "anomalous" stellar population. The two dominant populations exhibit the same [Na/Fe]-[Al/Fe] correlation found in other "normal" globular clusters. Therefore, NGC 6273 joins ω Centauri, M 22, M 2, and NGC 5286 as a possible new class of Galactic globular clusters.

Put your hands up! Gesturing improves preschoolers' executive function.

PubMed

Rhoads, Candace L; Miller, Patricia H; Jaeger, Gina O

2018-09-01

This study addressed the causal direction of a previously reported relation between preschoolers' gesturing and their executive functioning on the Dimensional Change Card Sort (DCCS) sorting-switch task. Gesturing the relevant dimension for sorting was induced in a Gesture group through instructions, imitation, and prompts. In contrast, the Control group was instructed to "think hard" when sorting. Preschoolers (N = 50) performed two DCCS tasks: (a) sort by size and then spatial orientation of two objects and (b) sort by shape and then proximity of the two objects. An examination of performance over trials permitted a fine-grained depiction of patterns of younger and older children in the Gesture and Control conditions. After the relevant dimension was switched, the Gesture group had more accurate sorts than the Control group, particularly among younger children on the second task. Moreover, the amount of gesturing predicted the number of correct sorts among younger children on the second task. The overall association between gesturing and sorting was not reflected at the level of individual trials, perhaps indicating covert gestural representation on some trials or the triggering of a relevant verbal representation by the gesturing. The delayed benefit of gesturing, until the second task, in the younger children may indicate a utilization deficiency. Results are discussed in terms of theories of gesturing and thought. The findings open up a new avenue of research and theorizing about the possible role of gesturing in emerging executive function. Copyright © 2018 Elsevier Inc. All rights reserved.

[CD34(+)/CD123(+) cell sorting from the patients with leukemia by Midi MACS method].

PubMed

Wang, Guang-Ping; Cao, Xin-Yu; Xin, Hong-Ya; Li, Qun; Qi, Zhen-Hua; Chen, Fang-Ping

2006-10-01

The aim of this study was to sort the CD34(+)/CD123(+) cells from the bone marrow cells of patients with acute myeloid leukemia (AML) by Midi MACS method. Firstly, the bone marrow mononuclear cells (BMMNC) were isolated from the patients with AML with Ficoll Paque, CD34(+) cells were then isolated by Midi MACS method followed by the isolation of CD34(+)/CD123(+) cells from the fraction of CD34(+) cells. The enrichment and recovery of CD34(+) and CD34(+)/CD123(+) cells were assayed by FACS technique. The results showed that the enrichment of CD34(+) cells was up to 98.73%, its average enrichment was 95.6%, and the recovery of CD34(+) was 84.6%, its average recovery was 51% after the first round sorting, by the second round sorting, the enrichment of CD34(+)/CD123(+) cells was up to 99.23%, its average enrichment was 83%. With regard to BMMNCs before sorting, the recovery of CD34(+)/CD123(+) was 34%. But, on the CD34(+) cells obtained by the first round sorting, its recovery was 56%. In conclusion, these results confirmed that the method of Midi MACS sorting can be applied to sort CD34(+)/CD123(+) cells from the bone marrow cells of AML patients, which give rise to the similar enrichment and recovery of the sorted cells with that of literature reported by the method of FACS.

Perseveration and the Status of 3-Year-Olds' Knowledge in a Card-Sorting Task: Evidence from Studies Involving Congruent Flankers

ERIC Educational Resources Information Center

Jordan, Patricia L.; Morton, J. Bruce

2012-01-01

Infants and young children often perseverate despite apparent knowledge of the correct response. Two Experiments addressed questions concerning the status of such knowledge in the context of a card-sorting task. In Experiment 1, three groups of 3-year-olds sorted bivalent cards one way and then were instructed to switch and sort the same cards…

Activation of epidermal growth factor receptor mediates receptor axon sorting and extension in the developing olfactory system of the moth Manduca sexta.

PubMed

Gibson, Nicholas J; Tolbert, Leslie P

2006-04-10

During development of the adult olfactory system of the moth Manduca sexta, olfactory receptor neurons extend axons from the olfactory epithelium in the antenna into the brain. As they arrive at the brain, interactions with centrally derived glial cells cause axons to sort and fasciculate with other axons destined to innervate the same glomeruli. Here we report studies indicating that activation of the epidermal growth factor receptor (EGFR) is involved in axon ingrowth and targeting. Blocking the EGFR kinase domain pharmacologically leads to stalling of many axons in the sorting zone and nerve layer as well as abnormal axonal fasciculation in the sorting zone. We also find that neuroglian, an IgCAM known to activate the EGFR through homophilic interactions in other systems, is transiently present on olfactory receptor neuron axons and on glia during the critical stages of the sorting process. The neuroglian is resistant to extraction with Triton X-100 in the sorting zone and nerve layer, possibly indicating its stabilization by homophilic binding in these regions. Our results suggest a mechanism whereby neuroglian molecules on axons and possibly sorting zone glia bind homophilically, leading to activation of EGFRs, with subsequent effects on axon sorting, pathfinding, and extension, and glomerulus development. Copyright 2006 Wiley-Liss, Inc.

Activation of EGF Receptor Mediates Receptor Axon Sorting and Extension in the Developing Olfactory System of the Moth Manduca sexta

PubMed Central

Gibson, Nicholas J.; Tolbert, Leslie P.

2008-01-01

During development of the adult olfactory system of the moth Manduca sexta, olfactory receptor neurons extend axons from the olfactory epithelium in the antenna into the brain. As they arrive at the brain, interactions with centrally-derived glial cells cause axons to sort and fasciculate with other axons destined to innervate the same glomeruli. Here we report studies that indicate that activation of the epidermal growth factor receptor (EGFR) is involved in axon ingrowth and targeting. Blocking the EGFR kinase domain pharmacologically leads to stalling of many axons in the sorting zone and nerve layer, as well as abnormal axonal fasciculation in the sorting zone. We also find that neuroglian, an IgCAM known to activate the EGFR through homophilic interactions in other systems, is transiently present on olfactory receptor neuron axons and on glia during the critical stages of the sorting process. The neuroglian is resistant to extraction with Triton X-100 in the sorting zone and nerve layer, possibly indicating its stabilization by homophilic binding in these regions. Our results suggest a mechanism whereby neuroglian molecules on axons and possibly sorting zone glia bind homophilically, leading to activation of EGFRs with subsequent effects on axon sorting, pathfinding, and extension, and glomerulus development. PMID:16498681

Effect of staining and freezing media on sortability of stallion spermatozoa and their post-thaw viability after sex-sorting and cryopreservation.

PubMed

Clulow, J R; Buss, H; Evans, G; Sieme, H; Rath, D; Morris, L H A; Maxwell, W M C

2012-02-01

Sex-sorted, frozen-thawed stallion spermatozoa remain out of reach of commercial horse breeders because of the low efficiency of the sex-sorting process and unacceptable fertility rates after insemination. Two experiments were designed to test the effects of alternative staining and freezing media to improve the viability of sex-sorted frozen-thawed stallion spermatozoa. Experiment 1 compared two freezing media, INRA 82(®) and a modified lactose-ethylenediaminetetraacetic acid (EDTA), for the cryopreservation of sex-sorted stallion spermatozoa. No significant differences between the two freezing media could be identified, suggesting that both cryodiluents would be suitable for incorporation into a sex-preselection protocol for stallion spermatozoa. Experiment 2 compared Kenney's modified Tyrode's (KMT) and Sperm TALP (Sp-TALP) as the staining and incubation medium for stallion spermatozoa prior to sex-sorting. A significant increase in the percentage of acrosome-reacted spermatozoa occurred after staining and incubation in the clarified Sp-TALP compared with KMT. As no improvements in sorting rates were achieved using Sp-TALP, it was concluded that stallion sorting protocols could include KMT as the staining and incubation medium while either INRA 82(®) or lactose-EDTA could be employed as a cryodiluents. © 2011 Blackwell Verlag GmbH.

Sorted bedform pattern evolution: Persistence, destruction and self-organized intermittency

NASA Astrophysics Data System (ADS)

Goldstein, Evan B.; Murray, A. Brad; Coco, Giovanni

2011-12-01

We investigate the long-term evolution of inner continental shelf sorted bedform patterns. Numerical modeling suggests that a range of behaviors are possible, from pattern persistence to spatial-temporal intermittency. Sorted bedform persistence results from a robust sorting feedback that operates when the seabed features a sufficient concentration of coarse material. In the absence of storm events, pattern maturation processes such as defect dynamics and pattern migration tend to cause the burial of coarse material and excavation of fine material, leading to the fining of the active layer. Vertical sorting occurs until a critical state of active layer coarseness is reached. This critical state results in the local cessation of the sorting feedback, leading to a self-organized spatially intermittent pattern, a hallmark of observed sorted bedforms. Bedforms in shallow conditions and those subject to high wave climates may be temporally intermittent features as a result of increased wave orbital velocity during storms. Erosion, or deposition of bimodal sediment, similarly leads to a spatially intermittent pattern, with individual coarse domains exhibiting temporal intermittence. Recurring storm events cause coarsening of the seabed (strengthening the sorting feedback) and the development of large wavelength patterns. Cessation of storm events leads to the superposition of storm (large wavelength) and inter-storm (small wavelength) patterns and spatial heterogeneity of pattern modes.

Late Quaternary Paleohydrology of Arroyo Cycles in the Mixteca Alta, Mexico: Insights from Past and Present Alluvial Stratigraphy

NASA Astrophysics Data System (ADS)

Holdridge, Geneviève; Leigh, David S.

2017-04-01

Arroyos are incised, ephemeral dryland streams that aggrade and incise on a cyclical timescale. Arroyo studies are integral to understanding landscape evolution in drylands, but the drivers and dynamics of arroyo cycles are not fully understood. This paper presents a study of arroyo stratigraphy in the semi-arid environment of the Mixteca Alta, Oaxaca, Mexico. Examination of the late Quaternary environment and geomorphology of the incised, ephemeral tributaries forming the Río Culebra watershed offered insight about the hydrological variability of the region. The characteristics and chronology of alluvium-paleosol sequences from three watersheds in the Mixteca Alta were examined along their upper, middle and downstream sections. In addition, the channel bedforms and stratigraphy of several watersheds were surveyed to evaluate the processes and morphology characterizing the present dryland stream system. Alluvium-paleosol sequences offered valuable information into the paleohydrology and dynamics of the arroyo cycles of the Mixteca Alta. Unweathered alluvium and paleosol formation in alluvium are two end members of alluvial stratigraphy. Cumulic A horizons form on floodplains when aggradation is slow enough to allow for pedogenesis. Cumulic A horizons were observed in both the Culebra and Verde watersheds from the late Pleistocene to the late Holocene, but not in the present-day arroyos, suggesting the prehistoric cumulic A horizons form in less flashy conditions than modern conditions. The geomorphic survey of present-day processes and forms included observing the bed stratigraphy associated with scouring-chains after flash floods. This examination offered insight to understanding and interpreting the alluvium-paleosol stratigraphy. For example, the present channel bed stratigraphy associated with scour-and-fill consists of poorly sorted, massive, sandy gravel deposits. Finer bedded sediments are observed in the present patchy floodplain deposits, but are susceptible to erosion. In contrast, the Quaternary stratigraphy is dominated by extensive fine sediment deposits, having thin bedding and laminations, while coarser, massive strata are observed, but to a lesser extent. The less prevalent coarser strata are analogous to present-day flash floods deposits, whereas the more widespread finer strata indicates a different flood regime associated with arroyo fills. Comparing the alluvium-paleosol chronology of the Mixteca Alta watersheds against high-resolution records from regional paleoclimatic proxies, such as stalagmites, offered insight concerning the climatic regime associated with different alluvial sequences. For example, incision was typically associated with wet periods, while alluvial strata were deposited during relatively drier periods. In addition, land use activities interacted with climate to produce hydrologic changes at a local scale prior to the Post-Classic period (e.g., local Natividad phase (1,250-430 cal BP). Afterwards, widespread land use interacted with climate to affect hydrological changes on the watershed scale. The present system of Río Culebra appears to be in complex response, adjusting to the long-term and short-term influences of late Holocene climate variation, legacies of prehistoric land-use change, and modern land use activities.

Reassessing Catastrophic Infill of the Pokhara Valley, Nepal Himalaya

NASA Astrophysics Data System (ADS)

Schwanghart, Wolfgang; Bernhardt, Anne; Stolle, Amelie; Adhikari, Basantha; Korup, Oliver

2014-05-01

The Pokhara valley, home to Nepal's second largest city and a major tourist attraction (28°15'N, 83°58'E), is covered by 4-5 km3 and 50-100 m thick intramontane fan deposits that resulted from massive aggradation of the Seti Khola, a river draining the Annapurna Massif of the Greater Himalaya. Poorly sorted, gravelly fluvial facies intercalated with debris-flow and mud-flow facies known as the Pokhara Gravels attest to highly energetic transport conditions during one or several catastrophic flow events. In May 2012, a devastative flash flood/debris flow in the Seti Khola rekindled interest in the formation processes and timing of the Pokhara Gravels as they may provide constraints on the magnitudes and frequencies of similar past events. Interpretations of previous sedimentological work and radiocarbon dating (Yamanaka, 1982; Fort, 1987) culminated in the belief that the Pokhara Gravels were catastrophically emplaced only 500 to 1000 years ago, although the exact nature, timing, and triggers of the purported event(s) remain obscure. Specifically, it remains debated whether the Pokhara Gravels were deposited instantaneously, possibly within less than a year, or whether sedimentation was more protracted over perhaps decades to millennia. We present new geomorphological, sedimentological, geochemical, and radiocarbon data and re-assess a potential catastrophic infill of the Pokhara Valley during one or several high-magnitude events. Support for this scenario is given by laterally continuous long-runout (~40 km) debris-flow deposits topped by large (i.e. up to >11-m) boulders, a distinctly calcareous lithology diagnostic of a small Greater Himalayan source area tens of kilometres upstream, and by historical anecdotes of a large flood that destroyed an earlier settlement in the area. However, we show that dated outcrops of fine-grained sediments in tributaries blocked by the Pokhara Gravels yield asynchronous ages. Although our radiocarbon dates are consistent with previously reported ones, pooled ages may equally well reflect more than one depositional event. We infer that massive aggradation must have been ongoing after rivers began incising into the Pokhara Gravels. Yet, geochemical fingerprinting of stillwater sediments located several kilometers upstream in these and other tributary valleys suggests a common and strikingly dominant sediment source limited to the Seti Khola's glaciated headwaters. These findings are at odds with the sedimentology of the Pokhara Gravels that point at one or more phases of deposition, most likely by high-magnitude events, possibly even by different transport processes. In summary, our results call for a much more detailed enquiry into the timing and mode of emplacement of the Pokhara Gravels in order to avoid gross misestimates of the hazard portfolio of the Pokhara valley. References: A. Yamanaka. The Science Reports of Tohoku University, 7th Series (Geography), 32, 46-60 (1982). M. Fort. Zeitsch. f. Geom. N.F., Suppl., 63, 9-36 (1987).

A Sequence of Sorting Strategies.

ERIC Educational Resources Information Center

Duncan, David R.; Litwiller, Bonnie H.

1984-01-01

Describes eight increasingly sophisticated and efficient sorting algorithms including linear insertion, binary insertion, shellsort, bubble exchange, shakersort, quick sort, straight selection, and tree selection. Provides challenges for the reader and the student to program these efficiently. (JM)

Tsunami sediments and their grain size characteristics

NASA Astrophysics Data System (ADS)

Sulastya Putra, Purna

2018-02-01

Characteristics of tsunami deposits are very complex as the deposition by tsunami is very complex processes. The grain size characteristics of tsunami deposits are simply generalized no matter the local condition in which the deposition took place. The general characteristics are fining upward and landward, poor sorting, and the grain size distribution is not unimodal. Here I review the grain size characteristics of tsunami deposit in various environments: swale, coastal marsh and lagoon/lake. Review results show that although there are similar characters in some environments and cases, but in detail the characteristics in each environment can be distinguished; therefore, the tsunami deposit in each environment has its own characteristic. The local geological and geomorphological condition of the environment may greatly affect the grain size characteristics.

ADP and brucellosis indemnity systems development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sanders, W.M.; Harlan, B.L.

1976-01-01

Our initial study of the USDA/TAHC Brucellosis Indemnity Program in Texas has shown that both the efficiency and rate of claim payments can be increased by the application of present day computer technologies. Two main factors contribute to these increases: the number of discrepancies that are caused by poor penmanship, transposition of numbers, and other human errors can be monitored and minimized; and the documented information can be indexed, sorted, and searched faster, more efficiently, and without human error. The overall flow of documentation that is used to control the movement of infected or exposed animals through commerce should bemore » studied. A new system should be designed that fully utilizes present day computer and electronic technologies.« less

Evaluation of common cleaning and disinfection programmes in battery cage and on-floor layer houses in France.

PubMed

Huneau-Salaün, A; Michel, V; Balaine, L; Petetin, I; Eono, F; Ecobichon, F; Bouquin, S Le

2010-04-01

1. The aim in this study was to evaluate cleaning and disinfection programmes in battery cage and on-floor layer houses in France. 2. Cleaning and disinfection efficiency was assessed by a visual evaluation of cleaning and a bacteriological monitoring of surface contamination from counts of thermotolerant streptococci on contact agar plates. 3. In battery cage houses, dropping belts, manure conveyors, and house floors remained highly contaminated due to poor cleaning in half of the buildings examined. 4. In on-floor houses, a high standard of cleaning was achieved but errors in the planning of cleaning and disinfection operations sometimes led to a high residual contamination of nest boxes and egg sorting tables.

What does it take to turn a rock into a badlands material?

NASA Astrophysics Data System (ADS)

Kasanin-Grubin, Milica; Nadal Romero, Estela; Della Seta, Marta; Martinez-Murillo, Juan F.

2016-04-01

Badlands can develop under different climatic conditions ranging from arid to humid on materials that have a complex combination of physico-chemical properties. The aim of this study is to determine the critical material properties for badland development based on current knowledge and new data. For that purpose we analyzed, both using the existing and new data, the importance of the distribution of grain size, mineralogical composition, and physico-chemical properties. Generally, the badland materials are most commonly described as "fine - grained" however, the size of the dominant grain fractions is not the solely important parameter. We argue that there is a critical amount of each size fraction (sand, silt and clay) that makes these materials susceptible to erosion. Furthermore, sorting of the material is an important factor in material susceptibility to erosion. The well-sorted fine sediments are generally considered as materials prone to disintegration and piping, while sediments with a large range of sizes and higher degree of packing are more resistant. However, poorly sorted sediments can also be very erodible and are found in badlands. Besides quartz, feldspar and carbonates, clay minerals are always present in badland materials and these minerals are crucial for badland development.The dominant clay mineral determines the behaviour of badland material, regarding swelling/shrinking, dispersion and crust development. Previous studies have shown that pH, SAR (sodium adsorption ratio), TDS (total dissolved salts), PS (percentage of sodium) and ESP (exchangeable sodium percentage) are distinctive parameters for both eroded and non-eroded slopes in badlands. Furthermore, our findings prove that content of organic carbon (Corg) is also a very important parameter and that materials with high SAR are less dispersive if the Corg is above 3%. In conclusion, this study shows that there are a number of thresholds regarding grain size, mineralogical composition and physico-chemical parameters that have to be met to make sediment a badland material.

Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells

PubMed Central

Johnson, Kristen E.; Mitra, Shalini; Katoch, Parul; Kelsey, Linda S.; Johnson, Keith R.; Mehta, Parmender P.

2013-01-01

The molecular mechanisms regulating the assembly of connexins (Cxs) into gap junctions are poorly understood. Using human pancreatic tumor cell lines BxPC3 and Capan-1, which express Cx26 and Cx43, we show that, upon arrival at the cell surface, the assembly of Cx43 is impaired. Connexin43 fails to assemble, because it is internalized by clathrin-mediated endocytosis. Assembly is restored upon expressing a sorting-motif mutant of Cx43, which does not interact with the AP2 complex, and by expressing mutants that cannot be phosphorylated on Ser-279 and Ser-282. The mutants restore assembly by preventing clathrin-mediated endocytosis of Cx43. Our results also document that the sorting-motif mutant is assembled into gap junctions in cells in which the expression of endogenous Cx43 has been knocked down. Remarkably, Cx43 mutants that cannot be phosphorylated on Ser-279 or Ser-282 are assembled into gap junctions only when connexons are composed of Cx43 forms that can be phosphorylated on these serines and forms in which phosphorylation on these serines is abolished. Based on the subcellular fate of Cx43 in single and contacting cells, our results document that the endocytic itinerary of Cx43 is altered upon cell–cell contact, which causes Cx43 to traffic by EEA1-negative endosomes en route to lysosomes. Our results further show that gap-junctional plaques formed of a sorting motif–deficient mutant of Cx43, which is unable to be internalized by the clathrin-mediated pathway, are predominantly endocytosed in the form of annular junctions. Thus the differential phosphorylation of Cx43 on Ser-279 and Ser-282 is fine-tuned to control Cx43’s endocytosis and assembly into gap junctions. PMID:23363606

Mice with Sort1 deficiency display normal cognition but elevated anxiety-like behavior.

PubMed

Ruan, Chun-Sheng; Yang, Chun-Rui; Li, Jia-Yi; Luo, Hai-Yun; Bobrovskaya, Larisa; Zhou, Xin-Fu

2016-07-01

Exposure to stressful life events plays a central role in the development of mood disorders in vulnerable individuals. However, the mechanisms that link mood disorders to stress are poorly understood. Brain-derived neurotrophic factor (BDNF) has long been implicated in positive regulation of depression and anxiety, while its precursor (proBDNF) recently showed an opposing effect on such mental illnesses. P75(NTR) and sortilin are co-receptors of proBDNF, however, the role of these receptors in mood regulation is not established. Here, we aimed to investigate the role of sortilin in regulating mood-related behaviors and its role in the proBDNF-mediated mood abnormality in mice. We found that sortilin was up-regulated in neocortex (by 78.3%) and hippocampus (by 111%) of chronically stressed mice as assessed by western blot analysis. These changes were associated with decreased mobility in the open field test and increased depression-like behavior in the forced swimming test. We also found that sortilin deficiency in mice resulted in hyperlocomotion in the open field test and increased anxiety-like behavior in both the open field and elevated plus maze tests. No depression-like behavior in the forced swimming test and no deficit in spatial cognition in the Morris water maze test were found in the Sort1-deficient mice. Moreover, the intracellular and extracellular levels of mature BDNF and proBDNF were not changed when sortilin was absent in vivo and in vitro. Finally, we found that both WT and Sort1-deficient mice injected with proBDNF in lateral ventricle displayed increased depression-like behavior in the forced swimming test but not anxiety-like behaviors in the open field and elevated plus maze tests. The present study suggests that sortilin functions as a negative regulator of mood performance and can be a therapeutic target for the treatment of mental illness. Crown Copyright © 2016. Published by Elsevier Inc. All rights reserved.

Creation and the empirical validation of the dignity card-sort tool to assess factors influencing erosion of dignity at life's end.

PubMed

Periyakoil, Vyjeyanthi S; Kraemer, Helena Chmura; Noda, Arthur

2009-12-01

Patients often experience erosion of dignity as they cope with the dying process. Preserving patient dignity is a sentinel premise of palliative care. This study was conducted to gain a better understanding of factors influencing erosion of dignity at the end of life. We conducted an open-ended written survey of 100 multidisciplinary providers (69% response rate) and responses were categorized to identify 18 themes that were used to create a card-sort tool. The initial 18-item tool was administered to nurses (n = 83), nonhospice community-dwelling subjects (n = 190) and hospice patients (n = 26) and a principal component analysis (PCA) was used to identify the 6 primary factors. The key item in each factor as identified by the PCA was used to create the final 6-item dignity card-sort tool (DCT). The DCT was also administered to physicians caring for palliative care patients (n = 21). For each of the final 6 items, the correlation between the respondents (nurses, physicians, nonterminally ill subjects, and subjects receiving hospice care) was calculated using the Spearman's correlation coefficient. The nurses were very highly positively correlated with the physicians (correlation coefficient = 0.94) and the community-dwelling nonterminally ill subjects were highly positively correlated with the subjects receiving hospice care (correlation coefficient = 0.67). More importantly, both the nurses and physicians were negatively correlated with both community dwelling nonterminally ill subjects and the subjects receiving hospice care. The health professionals in the study felt that treating a patient with disrespect and not carrying out their wishes resulted in erosion of dignity. In contrast patients thought that poor medical care and untreated pain were the most important factors leading to erosion of dignity at life's end. The DCT is a promising tool that may help clinicians identify key factors resulting in perceptions of erosion of dignity in adult palliative care patients.

Creation and the Empirical Validation of the Dignity Card-Sort Tool To Assess Factors Influencing Erosion of Dignity at Life's End

PubMed Central

Chmura Kraemer, Helena; Noda, Arthur

2009-01-01

Abstract Patients often experience erosion of dignity as they cope with the dying process. Preserving patient dignity is a sentinel premise of palliative care. This study was conducted to gain a better understanding of factors influencing erosion of dignity at the end of life. We conducted an open-ended written survey of 100 multidisciplinary providers (69% response rate) and responses were categorized to identify 18 themes that were used to create a card-sort tool. The initial 18-item tool was administered to nurses (n = 83), nonhospice community-dwelling subjects (n = 190) and hospice patients (n = 26) and a principal component analysis (PCA) was used to identify the 6 primary factors. The key item in each factor as identified by the PCA was used to create the final 6-item dignity card-sort tool (DCT). The DCT was also administered to physicians caring for palliative care patients (n = 21). For each of the final 6 items, the correlation between the respondents (nurses, physicians, nonterminally ill subjects, and subjects receiving hospice care) was calculated using the Spearman's correlation coefficient. The nurses were very highly positively correlated with the physicians (correlation coefficient = 0.94) and the community-dwelling nonterminally ill subjects were highly positively correlated with the subjects receiving hospice care (correlation coefficient = 0.67). More importantly, both the nurses and physicians were negatively correlated with both community dwelling nonterminally ill subjects and the subjects receiving hospice care. The health professionals in the study felt that treating a patient with disrespect and not carrying out their wishes resulted in erosion of dignity. In contrast patients thought that poor medical care and untreated pain were the most important factors leading to erosion of dignity at life's end. The DCT is a promising tool that may help clinicians identify key factors resulting in perceptions of erosion of dignity in adult palliative care patients. PMID:19708793

UBE4B Protein Couples Ubiquitination and Sorting Machineries to Enable Epidermal Growth Factor Receptor (EGFR) Degradation*

PubMed Central

Sirisaengtaksin, Natalie; Gireud, Monica; Yan, Qing; Kubota, Yoshihisa; Meza, Denisse; Waymire, Jack C.; Zage, Peter E.; Bean, Andrew J.

2014-01-01

The signaling of plasma membrane proteins is tuned by internalization and sorting in the endocytic pathway prior to recycling or degradation in lysosomes. Ubiquitin modification allows recognition and association of cargo with endosomally associated protein complexes, enabling sorting of proteins to be degraded from those to be recycled. The mechanism that provides coordination between the cellular machineries that mediate ubiquitination and endosomal sorting is unknown. We report that the ubiquitin ligase UBE4B is recruited to endosomes in response to epidermal growth factor receptor (EGFR) activation by binding to Hrs, a key component of endosomal sorting complex required for transport (ESCRT) 0. We identify the EGFR as a substrate for UBE4B, establish UBE4B as a regulator of EGFR degradation, and describe a mechanism by which UBE4B regulates endosomal sorting, affecting cellular levels of the EGFR and its downstream signaling. We propose a model in which the coordinated action of UBE4B, ESCRT-0, and the deubiquitinating enzyme USP8 enable the endosomal sorting and lysosomal degradation of the EGFR. PMID:24344129

Directed blasts and blast-generated pyroclastic density currents: a comparison of the Bezymianny 1956, Mount St Helens 1980, and Soufrière Hills, Montserrat 1997 eruptions and deposits

USGS Publications Warehouse

Belousov, Alexander; Voight, Barry; Belousova, Marina

2007-01-01

We compare eruptive dynamics, effects and deposits of the Bezymianny 1956 (BZ), Mount St Helens 1980 (MSH), and Soufrière Hills volcano, Montserrat 1997 (SHV) eruptions, the key events of which included powerful directed blasts. Each blast subsequently generated a high-energy stratified pyroclastic density current (PDC) with a high speed at onset. The blasts were triggered by rapid unloading of an extruding or intruding shallow magma body (lava dome and/or cryptodome) of andesitic or dacitic composition. The unloading was caused by sector failures of the volcanic edifices, with respective volumes for BZ, MSH, and SHV c. 0.5, 2.5, and 0.05 km3 . The blasts devastated approximately elliptical areas, axial directions of which coincided with the directions of sector failures. We separate the transient directed blast phenomenon into three main parts, the burst phase, the collapse phase, and the PDC phase. In the burst phase the pressurized mixture is driven by initial kinetic energy and expands rapidly into the atmosphere, with much of the expansion having an initially lateral component. The erupted material fails to mix with sufficient air to form a buoyant column, but in the collapse phase, falls beyond the source as an inclined fountain, and thereafter generates a PDC moving parallel to the ground surface. It is possible for the burst phase to comprise an overpressured jet, which requires injection of momentum from an orifice; however some exploding sources may have different geometry and a jet is not necessarily formed. A major unresolved question is whether the preponderance of strong damage observed in the volcanic blasts should be attributed to shock waves within an overpressured jet, or alternatively to dynamic pressures and shocks within the energetic collapse and PDC phases. Internal shock structures related to unsteady flow and compressibility effects can occur in each phase. We withhold judgment about published shock models as a primary explanation for the damage sustained at MSH until modern 3D numerical modeling is accomplished, but argue that much of the damage observed in directed blasts can be reasonably interpreted to have been caused by high dynamic pressures and clast impact loading by an inclined collapsing fountain and stratified PDC. This view is reinforced by recent modeling cited for SHV. In distal and peripheral regions, solids concentration, maximum particle size, current speed, and dynamic pressure are diminished, resulting in lesser damage and enhanced influence by local topography on the PDC. Despite the different scales of the blasts (devastated areas were respectively 500, 600, and >10 km2 for BZ, MSH, and SHV), and some complexity involving retrogressive slide blocks and clusters of explosions, their pyroclastic deposits demonstrate strong similarity. Juvenile material composes >50% of the deposits, implying for the blasts a dominantly magmatic mechanism although hydrothermal explosions also occurred. The character of the magma fragmented by explosions (highly viscous, phenocryst-rich, variable microlite content) determined the bimodal distributions of juvenile clast density and vesicularity. Thickness of the deposits fluctuates in proximal areas but in general decreases with distance from the crater, and laterally from the axial region. The proximal stratigraphy of the blast deposits comprises four layers named A, B, C, D from bottom to top. Layer A is represented by very poorly sorted debris with admixtures of vegetation and soil, with a strongly erosive ground contact; its appearance varies at different sites due to different ground conditions at the time of the blasts. The layer reflects intense turbulent boundary shear between the basal part of the energetic head of the PDC and the substrate. Layer B exhibits relatively well-sorted fines depleted debris with some charred plant fragments; its deposition occurred by rapid suspension sedimentation in rapidly waning, high-concentration conditions. Layer C is mainly a poorly sorted massive layer enriched by fines with its uppermost part laminated, created by rapid sedimentation under moderate-concentration, weakly tractive conditions, with the uppermost laminated part reflecting a dilute depositional regime with grain-by-grain traction deposition. By analogy to laboratory experiments, mixing at the flow head of the PDC created a turbulent dilute wake above the body of a gravity current, with layer B deposited by the flow body and layer C by the wake. The uppermost layer D of fines and accretionary lapilli is an ash fallout deposit of the finest particles from the high-rising buoyant thermal plume derived from the sediment-depleted pyroclastic density current. The strong similarity among these eruptions and their deposits suggests that these cases represent similar source, transport and depositional phenomena.

Statistical Characteristics of Single Sort of Grape Bulgarian Wines

NASA Astrophysics Data System (ADS)

Boyadzhiev, D.

2008-10-01

The aim of this paper is to evaluate the differences in the values of the 8 basic physicochemical indices of single sort of grape Bulgarian wines (white and red ones), obligatory for the standardization of ready production in the winery. Statistically significant differences in the values of various sorts and vintages are established and possibilities for identifying the sort and the vintage on the base of these indices by applying discriminant analysis are discussed.

Cloning of Plasmodium falciparum by single-cell sorting

PubMed Central

Miao, Jun; Li, Xiaolian; Cui, Liwang

2010-01-01

Malaria parasite cloning is traditionally carried out mainly by using the limiting dilution method, which is laborious, imprecise, and unable to distinguish multiply-infected RBCs. In this study, we used a parasite engineered to express green fluorescent protein (GFP) to evaluate a single-cell sorting method for rapidly cloning Plasmodium falciparum. By dividing a two dimensional scattergram from a cell sorter into 17 gates, we determined the parameters for isolating singly-infected erythrocytes and sorted them into individual cultures. Pre-gating of the engineered parasites for GFP allowed the isolation of almost 100% GFP-positive clones. Compared with the limiting dilution method, the number of parasite clones obtained by single-cell sorting was much higher. Molecular analyses showed that parasite isolates obtained by single-cell sorting were highly homogenous. This highly efficient single-cell sorting method should prove very useful for cloning both P. falciparum laboratory populations from genetic manipulation experiments and clinical samples. PMID:20435038

PhySortR: a fast, flexible tool for sorting phylogenetic trees in R.

PubMed

Stephens, Timothy G; Bhattacharya, Debashish; Ragan, Mark A; Chan, Cheong Xin

2016-01-01

A frequent bottleneck in interpreting phylogenomic output is the need to screen often thousands of trees for features of interest, particularly robust clades of specific taxa, as evidence of monophyletic relationship and/or reticulated evolution. Here we present PhySortR, a fast, flexible R package for classifying phylogenetic trees. Unlike existing utilities, PhySortR allows for identification of both exclusive and non-exclusive clades uniting the target taxa based on tip labels (i.e., leaves) on a tree, with customisable options to assess clades within the context of the whole tree. Using simulated and empirical datasets, we demonstrate the potential and scalability of PhySortR in analysis of thousands of phylogenetic trees without a priori assumption of tree-rooting, and in yielding readily interpretable trees that unambiguously satisfy the query. PhySortR is a command-line tool that is freely available and easily automatable.

Sortilin 1 knockout alters basal adipose glucose metabolism but not diet-induced obesity in mice.

PubMed

Li, Jibiao; Matye, David J; Wang, Yifeng; Li, Tiangang

2017-04-01

Sortilin 1 (Sort1) is a trafficking receptor that has been implicated in the regulation of plasma cholesterol in humans and mice. Here, we use metabolomics and hyperinsulinemic-euglycemic clamp approaches to obtain further understanding of the in vivo effects of Sort1 deletion on diet-induced obesity as well as on adipose lipid and glucose metabolism. Results show that Sort1 knockout (KO) does not affect Western diet-induced obesity nor adipose fatty acid and ceramide concentrations. Under the basal fasting state, chow-fed Sort1 KO mice have decreased adipose glycolytic metabolites, but Sort1 deletion does not affect insulin-stimulated tissue glucose uptake during the insulin clamp. These results suggest that Sort1 loss-of-function in vivo does not affect obesity development, but differentially modulates adipose glucose metabolism under fasting and insulin-stimulated states. © 2017 Federation of European Biochemical Societies.

Standard practice for cell sorting in a BSL-3 facility.

PubMed

Perfetto, Stephen P; Ambrozak, David R; Nguyen, Richard; Roederer, Mario; Koup, Richard A; Holmes, Kevin L

2011-01-01

Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.

Standard Practice for Cell Sorting in a BSL-3 Facility

PubMed Central

Perfetto, Stephen P.; Ambrozak, David R.; Nguyen, Richard; Roederer, Mario; Koup, Richard A.; Holmes, Kevin L.

2016-01-01

Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation. PMID:21116997

Data parallel sorting for particle simulation

NASA Technical Reports Server (NTRS)

Dagum, Leonardo

1992-01-01

Sorting on a parallel architecture is a communications intensive event which can incur a high penalty in applications where it is required. In the case of particle simulation, only integer sorting is necessary, and sequential implementations easily attain the minimum performance bound of O (N) for N particles. Parallel implementations, however, have to cope with the parallel sorting problem which, in addition to incurring a heavy communications cost, can make the minimun performance bound difficult to attain. This paper demonstrates how the sorting problem in a particle simulation can be reduced to a merging problem, and describes an efficient data parallel algorithm to solve this merging problem in a particle simulation. The new algorithm is shown to be optimal under conditions usual for particle simulation, and its fieldwise implementation on the Connection Machine is analyzed in detail. The new algorithm is about four times faster than a fieldwise implementation of radix sort on the Connection Machine.

The interactive electrode localization utility: software for automatic sorting and labeling of intracranial subdural electrodes

PubMed Central

Tang, Wei; Peled, Noam; Vallejo, Deborah I.; Borzello, Mia; Dougherty, Darin D.; Eskandar, Emad N.; Widge, Alik S.; Cash, Sydney S.; Stufflebeam, Steven M.

2018-01-01

Purpose Existing methods for sorting, labeling, registering, and across-subject localization of electrodes in intracranial encephalography (iEEG) may involve laborious work requiring manual inspection of radiological images. Methods We describe a new open-source software package, the interactive electrode localization utility which presents a full pipeline for the registration, localization, and labeling of iEEG electrodes from CT and MR images. In addition, we describe a method to automatically sort and label electrodes from subdural grids of known geometry. Results We validated our software against manual inspection methods in twelve subjects undergoing iEEG for medically intractable epilepsy. Our algorithm for sorting and labeling performed correct identification on 96% of the electrodes. Conclusions The sorting and labeling methods we describe offer nearly perfect performance and the software package we have distributed may simplify the process of registering, sorting, labeling, and localizing subdural iEEG grid electrodes by manual inspection. PMID:27915398

Eta Carinae and the Homunculus: An Astrophysical Laboratory

NASA Technical Reports Server (NTRS)

Gull, Theodore R.

2006-01-01

High spatial resolution spectroscopy with HST/STIS between 1998.0 and 2004.2 has provided much exciting information about the central binary system and the physics of its N-rich, C,O-poor ejecta. Stellar He I profiles, noticeably blue-shifted relative to P Cygni H and Fe II line profiles, originate from the ionized wind region between two massive companions. Changes in profiles of He I singlet and triplet lines provide clues to the excitation mechanisms involved as the hot, UV companion moves in its highly eccentric orbit. For 90% of the 5.54-year period, the spectra of nearby Weigelt blobs and the Little Homunculus include highly excited emission lines of Ar, Ne, and Fe. During the few month-long spectroscopic minimum, these systems are deprived of Lyman continuum. Recombination, plus cooling, occurs. In the skirt region between the bipolar Homunculus, a neutral emission region, devoid of hydrogen emission, glows in Ti II, Fe I, Sr II, Sc II, etc. We find the ejecta to have Ti/Ni abundances nearly 100 times solar, not due to nuclear processing, but due to lack of oxygen. Many metals normally tied up in interstellar dust remain in gaseous phase. Much information is being obtained on the physical processes in these warm N-rich gases, whose excitation varies with time in a predictable pattern. Indeed recent GRB high dispersion spectra include signatures of circumGRB warm gases. This indicates that the early, primordial massive stars have warm massive ejecta reminiscent to that around Eta Carinae.

ATLASGAL -- A molecular view of an unbiased sample of massive star forming clumps

NASA Astrophysics Data System (ADS)

Figura, Charles; Urquhart, James; Wyrowski, Friedrich; Giannetti, Andrea; Kim, Wonju

2018-01-01

Massive stars play an important role in many areas of astrophysics, from regulating star formation to driving the evolution of their host galaxy. Study of these stars is made difficult by their short evolutionary timescales, small populations and greater distances, and further complicated because they reach the main sequence while still shrouded in their natal clumps. As a result, many aspects of their formation are still poorly understood.We have assembled a large and statistically representative collection of massive star-forming environments that span all evolutionary stages of development by correlating mid-infrared and dust continnum surveys. We have conducted follow-up single-pointing observations toward a sample of approximately 600 of these clumps with the Mopra telescope using an 8 GHz bandwidth that spans some 27 molecular and mm-radio recombination line transitions. These lines trace a wide range of interstellar conditions with varying thermal, chemical, and kinematic properties. Many of these lines exhibit hyperfine structure allowing more detailed measurements of the clump environment (e.g. rotation temperatures and column densities).From these twenty-seven lines, we have identified thirteen line intensity ratios that strongly trace the evolutionary state of these clumps. We have investigated individual molecular and mm-radio recombination lines, contrasting these with radio and sub-mm continuum observations. We present a summary of the results of the statistical analysis of the sample, and compare them with previous similar studies to test their utility as chemical clocks of the evolutionary processes.

Infrared outbursts as potential tracers of common-envelope events in high-mass X-ray binary formation

NASA Astrophysics Data System (ADS)

Oskinova, Lidia M.; Bulik, Tomasz; Gómez-Morán, Ada Nebot

2018-06-01

Context. Classic massive binary evolutionary scenarios predict that a transitional common-envelope (CE) phase could be preceded as well as succeeded by the evolutionary stage when a binary consists of a compact object and a massive star, that is, a high-mass X-ray binary (HMXB). The observational manifestations of common envelope are poorly constrained. We speculate that its ejection might be observed in some cases as a transient event at mid-infrared (IR) wavelengths. Aims: We estimate the expected numbers of CE ejection events and HMXBs per star formation unit rate, and compare these theoretical estimates with observations. Methods: We compiled a list of 85 mid-IR transients of uncertain nature detected by the Spitzer Infrared Intensive Transients Survey and searched for their associations with X-ray, optical, and UV sources. Results: Confirming our theoretical estimates, we find that only one potential HMXB may be plausibly associated with an IR-transient and tentatively propose that X-ray source NGC 4490-X40 could be a precursor to the SPIRITS 16az event. Among other interesting sources, we suggest that the supernova remnant candidate [BWL2012] 063 might be associated with SPIRITS 16ajc. We also find that two SPIRITS events are likely associated with novae, and seven have potential optical counterparts. Conclusions: The massive binary evolutionary scenarios that involve CE events do not contradict currently available observations of IR transients and HMXBs in star-forming galaxies.

Probing the Evolution of Massive Young Stellar Objects using Weak Class II 6.7GHz Methanol Maser Emission

NASA Astrophysics Data System (ADS)

Ludwig, Bethany Ann; Cunningham, Nichol

2017-01-01

We present results from an investigation of class II 6.7GHz methanol masers towards four Massive Young Stellar Objects (MYSOs). The sources, selected from the Red MSX Source (RMS) Survey (Lumsden et al. 2013), were previously understood to be non-detections for class II methanol maser emission in the methanol multi-beam (MMB) Survey (Caswell et al. 2010.) Class II methanol masers are a well-known sign post of massive star forming regions and may be utilized to probe their relatively poorly understood formation. It is possible that these non-detections are simply weak masers that are potentially associated with a younger evolutionary phase of MYSOs as hypothesized by Olmi et al. (2014). The sources were chosen to sample various stages of evolution, having similar 21 to 8 micron flux ratios and bolometric luminosities as other MYSOs with previous class II methanol maser detections. We observed all 4 MYSOs with ATCA (~2" resolution) at 10 times deeper sensitivity than previously obtained with the MMB survey and have a spectral resolution of 0.087kms^-1 . The raw data is reduced using the program Miriad (Sault, R. J., et al., 1995) and deconvolutioned using the program CASA (McMullin, J. P., et al. 2007.) We determine one of the four observed MYSOs is harboring a weak class II methanol maser. We discuss the possibility of sensitivity limitations on the remaining sources as well as environmental and evolutionary differences between the sources.

Spectral Confirmation of New Galactic LBV and WN Stars Associated With Mid-IR Nebulae

NASA Astrophysics Data System (ADS)

Stringfellow, Guy; Gvaramadze, Vasilii V.

2014-08-01

Luminous Blue Variable (LBV) stars represent an extremely rare class and short-lived phase in the lives of very luminous massive stars with high mass loss rates. Extragalactic LBVs are responsible for producing false supernovae (SN), the SN Impostors, and have been directly linked with the progenitors of actual SN, indicating the LBV phase can be a final endpoint for massive star evolution. Yet only a few confirmed LBVs have been identified in the Galaxy. Their stellar evolution is poorly constrained by observations, and the physical reason for their unstable nature, both in terms of moderate spectral and photometric variability of a few magnitudes and the giant eruptions a la η Car that rival SN explosions, remains a mystery. Newly discovered mid-IR shells act as signposts, pointing to the central massive stars (LBV and Wolf-Rayet [WR] stars) that produced them. We have undertaken a spectroscopic survey of possible progenitor stars within these shells and are discovering that many are LBVs and WN-type WR transitional stars. We propose to extend this IR spectral survey to the south to search for new progenitor stars associated with dozens of newly identified shells. This survey should result in a substantial increase of new WRs and candidate LBVs for continued future study. Spectral analysis will yield new insights into the winds and physical properties of these rare and important objects, and lead to a better understanding of the physics driving giant eruptions.

Towards a coherent view of mass loss in Betelgeuse from the photosphere to the interstellar medium

NASA Astrophysics Data System (ADS)

Montarges, Miguel; Kervella, Pierre; Perrin, Guy

2013-06-01

Massive evolved stars contribute to the chemical enrichment of the interstellar medium (ISM), the Galaxy and ultimately the Universe through their mass loss. From the photosphere to the ISM, large convective motions, low surface gravity and high brightness combine to trigger an intense stellar wind. The released material evolves chemically as the distance from the star increases, and eventually forms dust particles. The involved physical and chemical processes are central to apprehend the cosmic impact of massive evolved stars, but they are poorly understood: the surface convection remains essentially uncharacterized, as the composition of the wind, and the role of the magnetic field is unknown. Betelgeuse is the closest red supergiant and therefore it stands out as the best candidate to obtain detailed observations of the close circumstellar environment of a massive evolved star. We are currently running a high angular resolution observations program to obtain a multi-wavelength and multi-epoch characterisation of this star. Using the IOTA interferometer, Haubois et al. reconstructed an image of the photosphere of the star. Within 1 to 100 stellar radii, the VLT and VLTI allowed us to probe the compact molecular envelope of Betelgeuse (the MOLsphere) in the near-infrared, and the extended dusty envelope in the thermal infrared domain. They were recently completed with HST/STIS observations of the chromosphere in the UV, whose location is surprisingly coincident with the MOLsphere. We will present a brief review of our recent results and of our ongoing work on Betelgeuse.

NGC 6362: THE LEAST MASSIVE GLOBULAR CLUSTER WITH CHEMICALLY DISTINCT MULTIPLE POPULATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mucciarelli, Alessio; Dalessandro, Emanuele; Ferraro, Francesco R.

2016-06-20

We present the first measure of Fe and Na abundances in NGC 6362, a low-mass globular cluster (GC) where first- and second-generation stars are fully spatially mixed. A total of 160 member stars (along the red giant branch (RGB) and the red horizontal branch (RHB)) were observed with the multi-object spectrograph FLAMES at the Very Large Telescope. We find that the cluster has an iron abundance of [Fe/H] = −1.09 ± 0.01 dex, without evidence of intrinsic dispersion. On the other hand, the [Na/Fe] distribution turns out to be intrinsically broad and bimodal. The Na-poor and Na-rich stars populate, respectively,more » the bluest and the reddest RGBs detected in the color–magnitude diagrams including the U filter. The RGB is composed of a mixture of first- and second-generation stars in a similar proportion, while almost all the RHB stars belong to the first cluster generation. To date, NGC 6362 is the least massive GC where both the photometric and spectroscopic signatures of multiple populations have been detected.« less

Temperature and atmospheric CO2 concentration estimates through the PETM using triple oxygen isotope analysis of mammalian bioapatite

PubMed Central

Gehler, Alexander; Pack, Andreas

2016-01-01

The Paleocene–Eocene Thermal Maximum (PETM) is a remarkable climatic and environmental event that occurred 56 Ma ago and has importance for understanding possible future climate change. The Paleocene–Eocene transition is marked by a rapid temperature rise contemporaneous with a large negative carbon isotope excursion (CIE). Both the temperature and the isotopic excursion are well-documented by terrestrial and marine proxies. The CIE was the result of a massive release of carbon into the atmosphere. However, the carbon source and quantities of CO2 and CH4 greenhouse gases that contributed to global warming are poorly constrained and highly debated. Here we combine an established oxygen isotope paleothermometer with a newly developed triple oxygen isotope paleo-CO2 barometer. We attempt to quantify the source of greenhouse gases released during the Paleocene–Eocene transition by analyzing bioapatite of terrestrial mammals. Our results are consistent with previous estimates of PETM temperature change and suggest that not only CO2 but also massive release of seabed methane was the driver for CIE and PETM. PMID:27354522

Radio transients from newborn black holes

NASA Astrophysics Data System (ADS)

Kashiyama, Kazumi; Hotokezaka, Kenta; Murase, Kohta

2018-05-01

We consider radio emission from a newborn black hole (BH), which is accompanied by a mini-disk with a mass of ≲ M⊙. Such a disk can be formed from an outer edge of the progenitor's envelope, especially for metal-poor massive stars and/or massive stars in close binaries. The disk accretion rate is typically super-Eddington and an ultrafast outflow with a velocity of ˜0.1-0.3 c will be launched into the circumstellar medium. The outflow forms a collisionless shock, and electrons are accelerated and emit synchrotron emission in radio bands with a flux of ˜ 10^{26-30} erg s^{-1} Hz^{-1} days to decades after the BH formation. The model predicts not only a fast UV/optical transient but also quasi-simultaneous inverse-Compton X-ray emission ˜ a few days after the BH formation, and the discovery of the radio counterpart with coordinated searches will enable us to identify this type of transients. The occurrence rate can be 0.1 - 10 % of the core-collapse supernova rate, which makes them a promising target of dedicated radio observations such as the Jansky VLA Sky Survey.

Probing the mass assembly of massive nearby galaxies with deep imaging

NASA Astrophysics Data System (ADS)

Duc, P.-A.; Cuillandre, J.-C.; Alatalo, K.; Blitz, L.; Bois, M.; Bournaud, F.; Bureau, M.; Cappellari, M.; Côté, P.; Davies, R. L.; Davis, T. A.; de Zeeuw, P. T.; Emsellem, E.; Ferrarese, L.; Ferriere, E.; Gwyn, S.; Khochfar, S.; Krajnovic, D.; Kuntschner, H.; Lablanche, P.-Y.; McDermid, R. M.; Michel-Dansac, L.; Morganti, R.; Naab, T.; Oosterloo, T.; Sarzi, M.; Scott, N.; Serra, P.; Weijmans, A.; Young, L. M.

2013-07-01

According to a popular scenario supported by numerical models, the mass assembly and growth of massive galaxies, in particular the Early-Type Galaxies (ETGs), is, below a redshift of 1, mainly due to the accretion of multiple gas-poor satellites. In order to get observational evidence of the role played by minor dry mergers, we are obtaining extremely deep optical images of a complete volume limited sample of nearby ETGs. These observations, done with the CFHT as part of the ATLAS3D, NGVS and MATLAS projects, reach a stunning 28.5 - 29 mag.arcsec-2 surface brightness limit in the g' band. They allow us to detect the relics of past collisions such as faint stellar tidal tails as well as the very extended stellar halos which keep the memory of the last episodes of galactic accretion. Images and preliminary results from this on-going survey are presented, in particular a possible correlation between the fine structure index (which parametrizes the amount of tidal perturbation) of the ETGs, their stellar mass, effective radius and gas content.

Unveiling the transcriptional features associated with coccolithovirus infection of natural Emiliania huxleyi blooms.

PubMed

Pagarete, António; Le Corguillé, Gildas; Tiwari, Bela; Ogata, Hiroyuki; de Vargas, Colomban; Wilson, William H; Allen, Michael J

2011-12-01

Lytic viruses have been implicated in the massive cellular lysis observed during algal blooms, through which they assume a prominent role in oceanic carbon and nutrient flows. Despite their impact on biogeochemical cycling, the transcriptional dynamics of these important oceanic events is still poorly understood. Here, we employ an oligonucleotide microarray to monitor host (Emiliania huxleyi) and virus (coccolithovirus) transcriptomic features during the course of E. huxleyi blooms induced in seawater-based mesocosm enclosures. Host bloom development and subsequent coccolithovirus infection was associated with a major shift in transcriptional profile. In addition to the expected metabolic requirements typically associated with viral infection (amino acid and nucleotide metabolism, as well as transcription- and replication-associated functions), the results strongly suggest that the manipulation of lipid metabolism plays a fundamental role during host-virus interaction. The results herein reveal the scale, so far massively underestimated, of the transcriptional domination that occurs during coccolithovirus infection in the natural environment. © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

­­Clastic Pipes on Mars: Evidence for a Near Surface Groundwater System

NASA Astrophysics Data System (ADS)

Wheatley, D. F.; Chan, M. A.; Okubo, C. H.

2017-12-01

Clastic pipes, a type of vertical, columnar injectite, occur throughout the terrestrial stratigraphic record and are identified across many Martian terrains. Terrestrial pipe analogs can aid in identifying clastic pipes on Mars to understand their formation processes and their implications for a past near-surface groundwater system. On Earth, clastic pipes form through fluidization of overpressurized sediment. Fluidization occurs when the upward frictional (i.e., drag) forces of escaping fluids overpower the downward acting gravitational force. To create the forces necessary for pipe formation requires overpressurization of a body of water-saturated porous media overlain by a low permeability confining layer. As the pressure builds, the confining layer eventually fractures and the escaping fluids fluidize the porous sediment causing the sediment to behave like a fluid. These specific formation conditions record evidence of a violent release of fluid-suspended sediment including brecciation of the host and sealing material, internal outward grading/sorting that results in a coarser-grained commonly better cemented outer rind, traction structures, and a cylindrical geometry. Pipes form self-organized, dispersed spatial relationships due to the efficient diffusion of overpressured zones in the subsurface and the expulsion of sediment under pressure. Martian pipes occur across the northern lowlands, dichotomy boundary, and southern highlands in various forms of erosional relief ranging from newer eruption structures to eroded cylindrical/conical mounds with raised rims to highly eroded mounds/hills. Similar to terrestrial examples, Martian pipes form in evenly-spaced, self-organized arrangements. The pipes are typically internally massive with a raised outer rim (interpreted as a sorted, coarser-grained, better-cemented rim). This evidence indicates that Martian pipes formed through fluidization, which requires a near-surface groundwater system. Pipes create a window into the subsurface by excavating subsurface sediment and waters. After emplacement, pipes can also act as fluid conduits, channeling post-depositional fluid flow. The preferential porosity and flow paths may make the pipes an ideal exploration target for microbial life.

Impact of respiratory-correlated CT sorting algorithms on the choice of margin definition for free-breathing lung radiotherapy treatments.

PubMed

Thengumpallil, Sheeba; Germond, Jean-François; Bourhis, Jean; Bochud, François; Moeckli, Raphaël

2016-06-01

To investigate the impact of Toshiba phase- and amplitude-sorting algorithms on the margin strategies for free-breathing lung radiotherapy treatments in the presence of breathing variations. 4D CT of a sphere inside a dynamic thorax phantom was acquired. The 4D CT was reconstructed according to the phase- and amplitude-sorting algorithms. The phantom was moved by reproducing amplitude, frequency, and a mix of amplitude and frequency variations. Artefact analysis was performed for Mid-Ventilation and ITV-based strategies on the images reconstructed by phase- and amplitude-sorting algorithms. The target volume deviation was assessed by comparing the target volume acquired during irregular motion to the volume acquired during regular motion. The amplitude-sorting algorithm shows reduced artefacts for only amplitude variations while the phase-sorting algorithm for only frequency variations. For amplitude and frequency variations, both algorithms perform similarly. Most of the artefacts are blurring and incomplete structures. We found larger artefacts and volume differences for the Mid-Ventilation with respect to the ITV strategy, resulting in a higher relative difference of the surface distortion value which ranges between maximum 14.6% and minimum 4.1%. The amplitude- is superior to the phase-sorting algorithm in the reduction of motion artefacts for amplitude variations while phase-sorting for frequency variations. A proper choice of 4D CT sorting algorithm is important in order to reduce motion artefacts, especially if Mid-Ventilation strategy is used. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Efficient sampling of parsimonious inversion histories with application to genome rearrangement in Yersinia.

PubMed

Miklós, István; Darling, Aaron E

2009-06-22

Inversions are among the most common mutations acting on the order and orientation of genes in a genome, and polynomial-time algorithms exist to obtain a minimal length series of inversions that transform one genome arrangement to another. However, the minimum length series of inversions (the optimal sorting path) is often not unique as many such optimal sorting paths exist. If we assume that all optimal sorting paths are equally likely, then statistical inference on genome arrangement history must account for all such sorting paths and not just a single estimate. No deterministic polynomial algorithm is known to count the number of optimal sorting paths nor sample from the uniform distribution of optimal sorting paths. Here, we propose a stochastic method that uniformly samples the set of all optimal sorting paths. Our method uses a novel formulation of parallel Markov chain Monte Carlo. In practice, our method can quickly estimate the total number of optimal sorting paths. We introduce a variant of our approach in which short inversions are modeled to be more likely, and we show how the method can be used to estimate the distribution of inversion lengths and breakpoint usage in pathogenic Yersinia pestis. The proposed method has been implemented in a program called "MC4Inversion." We draw comparison of MC4Inversion to the sampler implemented in BADGER and a previously described importance sampling (IS) technique. We find that on high-divergence data sets, MC4Inversion finds more optimal sorting paths per second than BADGER and the IS technique and simultaneously avoids bias inherent in the IS technique.

Calnuc Function in Endosomal Sorting of Lysosomal Receptors.

PubMed

Larkin, Heidi; Costantino, Santiago; Seaman, Matthew N J; Lavoie, Christine

2016-04-01

Calnuc is a ubiquitous Ca(2+)-binding protein present on the trans-Golgi network (TGN) and endosomes. However, the precise role of Calnuc in these organelles is poorly characterized. We previously highlighted the role of Calnuc in the transport of LRP9, a new member of a low-density lipoprotein (LDL) receptor subfamily that cycles between the TGN and endosomes. The objective of this study was to explore the role of Calnuc in the endocytic sorting of mannose-6-phosphate receptor (MPR) and Sortilin, two well-characterized lysosomal receptors that transit between the TGN and endosomes. Using biochemical and microscopy assays, we showed that Calnuc depletion [by small interfering RNA (siRNA)] causes the misdelivery to and degradation in lysosomes of cationic-independent mannose-6-phosphate receptor (CI-MPR) and Sortilin due to a defect in the endosomal recruitment of retromers, which are key components of the endosome-to-Golgi retrieval machinery. Indeed, we demonstrated that Calnuc depletion impairs the activation and membrane association of Rab7, a small G protein required for the endosomal recruitment of retromers. Overall, our data indicate a novel role for Calnuc in the endosome-to-TGN retrograde transport of lysosomal receptors through the regulation of Rab7 activity and the recruitment of retromers to endosomes. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Unusual folding and rolling of Glacio-Lacustrine sediments, Upper Fraser Canyon, British Columbia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baxter, S.

1987-05-01

Folding and rolling of graded but unconsolidated sediments by at least 720/sup 0/ produced a structure resembling a large Swiss roll about 6 ft wide and 4 ft high. The sediments were initially horizontal and well sorted, grading from coarse sands to fine silts. About 50 ft away, at the same level, the sediments include irregular layers of poorly sorted, ice-rafted pebbles and boulders. The sequence is unconformably overlain by till. The axis of folding appears to be parallel to the eastern wall of the Fraser Canyon. The outcrop is in the Stevens Pit (sand and gravel) immediately east ofmore » the Trans-Canada Highway, 2 mi south of Lytton, B.C., at an elevation of 1000 ft, approximately 600 ft above the present level of the Fraser River. The sands and silts accumulated in a lake adjacent to the east margin of a stagnant and relatively small glacier occupying the upper part of the Frazer Canyon. Partial or complete melting of small icebergs caused deposition of coarser material. A subsequent cooling trend led to an advance of the glacier, an advance which at this location caused some of the adjacent and by now frozen sediments to be rolled up like an old carpet. Further advance of the glacier caused it to override and thus preserve the deformed sequence.« less

Ground-water resources in Mendocino County, California

USGS Publications Warehouse

Farrar, C.D.

1986-01-01

Mendocino County includes about 3,500 sq mi of coastal northern California. Groundwater is the main source for municipal and individual domestic water systems and contributes significantly to irrigation. Consolidated rocks of the Franciscan Complex are exposed over most of the county. The consolidated rocks are commonly dry and generally supply < 5 gal/min of water to wells. Unconsolidated fill in the inland valleys consists of gravel, sand, silt, and clay. Low permeability in the fill caused by fine grain size and poor sorting limits well yields to less than 50 gal/min in most areas; where the fill is better sorted, yields of 1,000 gal/min can be obtained. Storage capacity estimates for the three largest basins are Ukiah Valley, 90,000 acre-ft; Little lake Valley, 35,000 acre-ft; and Laytonville Valley, 14,000 acre-ft. Abundant rainfall (35 to 56 in/yr) generally recharges these basins to capacity. Seasonal water level fluctuations since the 1950 's have been nearly constant, except during the 1976-77 drought. Chemical quality of water in basement rocks and valley fill is generally acceptable for most uses. Some areas along fault zones yield water with high boron concentrations ( <2 mg/L). Sodium chloride water with dissolved solids concentrations exceeding 1,000 mg/L is found in deeper parts of Little Lake Valley. (Author 's abstract)

Deposition model of a Miocene barred wave- and storm-dominated shoreface and shelf, southeastern Malay basin, offshore west Malaysia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ramli, N.

1986-01-01

The J sandstone is an important hydrocarbon-bearing reservoir in the southeastern part of the Malay basin. The lower and upper members of the J sandstone are composed of shoreface and offshore sediments. The shoreface sequence contains depositional structures characteristic of a barred wave- and storm-dominated shoreface. Each shoreface sequence is laterally associated with a series of stacked offshore bars. Offshore bars can be subdivided into proximal and distal types. Two types of proximal offshore bars have been identified: (1) proximal bars formed largely above fair-weather wave base (inner proximal bars), and (2) proximal bars formed below fair-weather wave base (outermore » proximal bars). The inner proximal bars are closely associated with the shoreface sequence and are similar to the middle and lower shoreface. The presence of poorly sorted, polymodal, very fine to very coarse-grained sandstone beneath well-sorted crestal sandstones of inner proximal bars suggests that these offshore bars may have been deposited rapidly by storms. The crests of the inner proximal offshore bars were subsequently reworked by fair-weather processes, and the crests of the outer proximal and distal offshore bars were reworked by waning storm currents and oscillatory waves. Thick marine shales overlying offshore bars contain isolated sheet sandstones. Each sheet sandstone exhibits features that may be characteristic of distal storm shelf deposits. 15 figures, 2 tables.« less

ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis

PubMed Central

Konopacki, Filip A.; Dwivedy, Asha; Bellon, Anaïs; Blower, Michael D.

2016-01-01

Endocytosis and local protein synthesis (LPS) act coordinately to mediate the chemotropic responses of axons, but the link between these two processes is poorly understood. The endosomal sorting complex required for transport (ESCRT) is a key regulator of cargo sorting in the endocytic pathway, and here we have investigated the role of ESCRT-II, a critical ESCRT component, in Xenopus retinal ganglion cell (RGC) axons. We show that ESCRT-II is present in RGC axonal growth cones (GCs) where it co-localizes with endocytic vesicle GTPases and, unexpectedly, with the Netrin-1 receptor, deleted in colorectal cancer (DCC). ESCRT-II knockdown (KD) decreases endocytosis and, strikingly, reduces DCC in GCs and leads to axon growth and guidance defects. ESCRT-II-depleted axons fail to turn in response to a Netrin-1 gradient in vitro and many axons fail to exit the eye in vivo. These defects, similar to Netrin-1/DCC loss-of-function phenotypes, can be rescued in whole (in vitro) or in part (in vivo) by expressing DCC. In addition, ESCRT-II KD impairs LPS in GCs and live imaging reveals that ESCRT-II transports mRNAs in axons. Collectively, our results show that the ESCRT-II-mediated endocytic pathway regulates both DCC and LPS in the axonal compartment and suggest that ESCRT-II aids gradient sensing in GCs by coupling endocytosis to LPS. PMID:27248654

Size-based cell sorting with a resistive pulse sensor and an electromagnetic pump in a microfluidic chip.

PubMed

Song, Yongxin; Li, Mengqi; Pan, Xinxiang; Wang, Qi; Li, Dongqing

2015-02-01

An electrokinetic microfluidic chip is developed to detect and sort target cells by size from human blood samples. Target-cell detection is achieved by a differential resistive pulse sensor (RPS) based on the size difference between the target cell and other cells. Once a target cell is detected, the detected RPS signal will automatically actuate an electromagnetic pump built in a microchannel to push the target cell into a collecting channel. This method was applied to automatically detect and sort A549 cells and T-lymphocytes from a peripheral fingertip blood sample. The viability of A549 cells sorted in the collecting well was verified by Hoechst33342 and propidium iodide staining. The results show that as many as 100 target cells per minute can be sorted out from the sample solution and thus is particularly suitable for sorting very rare target cells, such as circulating tumor cells. The actuation of the electromagnetic valve has no influence on RPS cell detection and the consequent cell-sorting process. The viability of the collected A549 cell is not impacted by the applied electric field when the cell passes the RPS detection area. The device described in this article is simple, automatic, and label-free and has wide applications in size-based rare target cell sorting for medical diagnostics. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Microfluidic droplet sorting using integrated bilayer micro-valves

NASA Astrophysics Data System (ADS)

Chen, Yuncong; Tian, Yang; Xu, Zhen; Wang, Xinran; Yu, Sicong; Dong, Liang

2016-10-01

This paper reports on a microfluidic device capable of sorting microfluidic droplets utilizing conventional bilayer pneumatic micro-valves as sorting controllers. The device consists of two micro-valves placed symmetrically on two sides of a sorting area, each on top of a branching channel at an inclined angle with respect to the main channel. Changes in transmitted light intensity, induced by varying light absorbance by each droplet, are used to divert the droplet from the sorting area into one of the three outlet channels. When no valve is activated, the droplet flows into the outlet channel in the direction of the main channel. When one of the valves is triggered, the flexible membrane of valve will first be deflected. Once the droplet leaves the detection point, the deflected membrane will immediately return to its default flattened position, thereby exerting a drawing pressure on the droplet and deviating it from its original streamline to the outlet on the same side as the valve. This sorting method will be particularly suitable for numerous large-scale integrated microfluidic systems, where pneumatic micro-valves are already used. Only few structural modifications are needed to achieve droplet sorting capabilities in these systems. Due to the mechanical nature of diverting energy applied to droplets, the proposed sorting method may induce only minimal interference to biological species or microorganisms encapsulated inside the droplets that may accompany electrical, optical and magnetic-based techniques.

Characterization of identification errors and uses in localization of poor modal correlation

NASA Astrophysics Data System (ADS)

Martin, Guillaume; Balmes, Etienne; Chancelier, Thierry

2017-05-01

While modal identification is a mature subject, very few studies address the characterization of errors associated with components of a mode shape. This is particularly important in test/analysis correlation procedures, where the Modal Assurance Criterion is used to pair modes and to localize at which sensors discrepancies occur. Poor correlation is usually attributed to modeling errors, but clearly identification errors also occur. In particular with 3D Scanning Laser Doppler Vibrometer measurement, many transfer functions are measured. As a result individual validation of each measurement cannot be performed manually in a reasonable time frame and a notable fraction of measurements is expected to be fairly noisy leading to poor identification of the associated mode shape components. The paper first addresses measurements and introduces multiple criteria. The error measures the difference between test and synthesized transfer functions around each resonance and can be used to localize poorly identified modal components. For intermediate error values, diagnostic of the origin of the error is needed. The level evaluates the transfer function amplitude in the vicinity of a given mode and can be used to eliminate sensors with low responses. A Noise Over Signal indicator, product of error and level, is then shown to be relevant to detect poorly excited modes and errors due to modal property shifts between test batches. Finally, a contribution is introduced to evaluate the visibility of a mode in each transfer. Using tests on a drum brake component, these indicators are shown to provide relevant insight into the quality of measurements. In a second part, test/analysis correlation is addressed with a focus on the localization of sources of poor mode shape correlation. The MACCo algorithm, which sorts sensors by the impact of their removal on a MAC computation, is shown to be particularly relevant. Combined with the error it avoids keeping erroneous modal components. Applied after removal of poor modal components, it provides spatial maps of poor correlation, which help localizing mode shape correlation errors and thus prepare the selection of model changes in updating procedures.

Arthroscopic Partial Repair of Irreparable Rotator Cuff Tears: Preoperative Factors Associated With Outcome Deterioration Over 2 Years.

PubMed

Shon, Min Soo; Koh, Kyoung Hwan; Lim, Tae Kang; Kim, Won Ju; Kim, Kyung Cheon; Yoo, Jae Chul

2015-08-01

Arthroscopic partial repair is a treatment option in irreparable large-to-massive rotator cuff tears without arthritic changes. However, there are indications that arthroscopic partial repair does not yield satisfactory outcomes. To report the clinical and radiographic results of arthroscopic partial repairs in patients with irreparable large-to-massive cuff tears. In addition, an analysis was performed regarding preoperative factors that may influence patient outcomes and patient-rated satisfaction over time. Case series; Level of evidence, 4. From 2005 to 2011, a total of 31 patients who underwent arthroscopic partial repair for irreparable large-to-massive cuff tears were retrospectively evaluated. Partial repair was defined as posterior cuff tissue repair with or without subscapularis tendon repair to restore the transverse force couple of the cuff. Pain visual analog scale (PVAS), questionnaire results (American Shoulder and Elbow Surgeons [ASES] and Simple Shoulder Test [SST]), and radiographic changes (acromiohumeral distance and degenerative change) were assessed preoperatively, at first follow-up (roughly 1 year postoperatively), and at final follow-up (>2 years postoperatively). Patients rated their satisfaction level at each postoperative follow-up as well. Preoperative factors that might influence outcomes, such as patient demographics, tear size, and fatty infiltration, were investigated. The preoperative, first follow-up, and final follow-up results for mean PVAS (5.13, 2.13, and 3.16, respectively) and questionnaires (ASES: 41.97, 76.37, and 73.78; SST: 3.61, 6.33, and 6.07, respectively) improved significantly (all P < .05). Radiographic evaluation showed no difference compared with preoperative status. Nevertheless, patient-rated satisfaction at final evaluation was inferior: 16 good responses ("very satisfied" and "satisfied") and 15 poor responses ("rather the same" and "dissatisfied"). Despite initial improvements in both groups (P < .05), patients with poor satisfaction demonstrated statistically significant deterioration in mean PVAS (from 2.07 to 4.67), questionnaire scores (ASES: from 74.56 to 59.80; SST: from 5.11 to 3.81), and acromiohumeral distance (from 7.19 to 5.06 mm) between the first and final follow-up (all P < .05). Patients with good satisfaction showed no significant difference or they improved (P > .05) from the first to the final follow-up. Among preoperative factors, fatty infiltration of the teres minor was identified as the only statistically significant factor affecting patient-rated satisfaction (P = .007). This study showed that arthroscopic partial repair may produce initial improvement in selected outcomes at 2-year follow-up. However, about half of the patients in the study were not satisfied with their outcomes, which had deteriorated over time. Preoperative fatty infiltration of the teres minor was the only factor that correlated with worse final outcomes and poor satisfaction after arthroscopic partial repair. © 2015 The Author(s).

Topographic controls on pyroclastic density current dynamics: Insight from 18 May 1980 deposits at Mount St. Helens, Washington (USA)

NASA Astrophysics Data System (ADS)

Brand, Brittany D.; Bendaña, Sylvana; Self, Stephen; Pollock, Nicholas

2016-07-01

Our ability to interpret the deposits of pyroclastic density currents (PDCs) is critical for understanding the transport and depositional processes that control PDC dynamics. This paper focuses on the influence of slope on flow dynamics and criticality as recorded in PDC deposits from the 18 May 1980 eruption of Mt. St. Helens (USA). PDC deposits are found along the steep flanks (10°-30°) and across the pumice plain ( 5°) up to 8 km north of the volcano. Granulometry, componentry and descriptions of depositional characteristics (e.g., bedform morphology) are recorded with distance from source. The pumice plain deposits are primarily thick (3-12 m), massive and poorly-sorted, and represent deposition from a series of concentrated PDCs. By contrast, the steep flank deposits are stratified to cross-stratified, suggesting deposition from PDCs where turbulence strongly influenced transport and depositional processes. We propose that acceleration of the concentrated PDCs along the steep flanks resulted in thinning of the concentrated, basal region of the current(s). Enhanced entrainment of ambient air, and autofluidization from upward fluxes of air from substrate interstices and plunging breakers across rugged, irregular topography further inflated the currents to the point that the overriding turbulent region strongly influenced transport and depositional mechanisms. Acceleration in combination with partial confinement in slot canyons and high surface roughness would also increase basal shear stress, further promoting shear and traction transport in the basal region of the current. Conditions along the steep flank resulted in supercritical flow, as recorded by regressive bedforms, which gradually transitioned to subcritical flow downstream as the concentrated basal region thickness increased as a function of decreasing slope and flow energy. We also find that (1) PDCs were erosive into the underlying granular substrate along high slopes (> 25°) where currents were partially confined in steep slot canyons, suggesting that basal shear stress is an important control on erosive capacity, and (2) bedform amplitude, wavelength and the presence of regressive bedforms increase with increasing slope and proximity to source along the steep flank, suggesting a link between bedform morphology, flow velocity, and flow criticality. While our results indicate that slope and irregular topography strongly influence PDC dynamics, criticality and erosive capacity, the influence of these conditions on ultimate flow runout distance is unclear. The work here also highlights the issue that relationships between the controls on bedform size and morphology in density stratified flows remain poorly constrained, limiting our ability to extract important information about the currents that produced them. These final two points warrant further exploration through the combination of field, experimental and numerical approaches.

Repeated ruminal acidosis challenges in lactating dairy cows at high and low risk for developing acidosis: feed sorting.

PubMed

Devries, T J; Dohme, F; Beauchemin, K A

2008-10-01

An experiment was conducted to determine whether the susceptibility of cows to ruminal acidosis influences feed sorting and whether feed sorting changes during a bout of ruminal acidosis. Eight ruminally cannulated cows were assigned to 1 of 2 acidosis risk levels: low risk (LR, mid-lactation cows fed a 60% forage diet) or high risk (HR, early lactation cows fed a 45% forage diet). As a result, diets were intentionally confounded with milk production to represent 2 different acidosis risk scenarios. Cows were exposed to an acidosis challenge in each of two 14-d periods. Each period consisted of 3 baseline days, a feed restriction day (restricting TMR to 50% of ad libitum intake), an acidosis challenge day (1-h meal of 4 kg of ground barley/wheat before allocating the TMR), and a recovery phase. Ruminal pH was measured continuously for the first 9 d of each period using an indwelling system. Feed and orts were sampled for 2 baseline days, on the challenge day, and 1 and 3 d after the challenge day for each cow and subjected to particle size analysis. The separator contained 3 screens (18, 9, and 1.18 mm) and a bottom pan to determine the proportion of long, medium, short, and fine particles, respectively. Sorting was calculated as the actual intake of each particle size fraction expressed as a percentage of the predicted intake of that fraction. All cows sorted against the longest and finest TMR particles and sorted for medium-length particles. Sorting was performed to a greater extent by the HR cows, and this sorting was related to low ruminal pH. Both HR and LR cows altered their sorting behavior in response to acidosis challenges. For the HR cows, severe acidosis was associated with increased sorting for the longer particles in the diet and against the shorter particles, likely to lessen the effects of the very.

Flow cytometric sorting of fresh and frozen-thawed spermatozoa in the western lowland gorilla (Gorilla gorilla gorilla).

PubMed

O'Brien, J K; Stojanov, T; Crichton, E G; Evans, K M; Leigh, D; Maxwell, W M C; Evans, G; Loskutoff, N M

2005-08-01

We adapted flow cytometry technology for high-purity sorting of X chromosome-bearing spermatozoa in the western lowland gorilla (Gorilla gorilla gorilla). Our objectives were to develop methodologies for liquid storage of semen prior to sorting, sorting of liquid-stored and frozen-thawed spermatozoa, and assessment of sorting accuracy. In study 1, the in vitro sperm characteristics of gorilla ejaculates from one male were unchanged (P > 0.05) after 8 hr of liquid storage at 15 degrees C in a non-egg yolk diluent (HEPES-buffered modified Tyrode's medium). In study 2, we examined the efficacy of sorting fresh and frozen-thawed spermatozoa using human spermatozoa as a model for gorilla spermatozoa. Ejaculates from one male were split into fresh and frozen aliquots. X-enriched samples derived from both fresh and frozen-thawed human semen were of high purity, as determined by fluorescence in situ hybridization (FISH; 90.7%+/-2.3%, overall), and contained a high proportion of morphologically normal spermatozoa (86.0%+/-1.0%, overall). In study 3, we processed liquid-stored semen from two gorillas for sorting using a modification of methods for human spermatozoa. The sort rate for enrichment of X-bearing spermatozoa was 7.3+/-2.5 spermatozoa per second. The X-enriched samples were of high purity (single-sperm PCR: 83.7%) and normal morphology (79.0%+/-3.9%). In study 4 we examined frozen-thawed gorilla semen, and the sort rate (8.3+/-2.9 X-bearing sperm/sec), purity (89.7%), and normal morphology (81.4%+/-3.4%) were comparable to those of liquid-stored semen. Depending on the male and the type of sample used (fresh or frozen-thawed), 0.8-2.2% of gorilla spermatozoa in the processed ejaculate were present in the X-enriched sample. These results demonstrate that fresh or frozen-thawed gorilla spermatozoa can be flow cytometrically sorted into samples enriched for X-bearing spermatozoa. Copyright 2005 Wiley-Liss, Inc.

An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

NASA Astrophysics Data System (ADS)

Girault, Mathias; Kim, Hyonchol; Arakawa, Hisayuki; Matsuura, Kenji; Odaka, Masao; Hattori, Akihiro; Terazono, Hideyuki; Yasuda, Kenji

2017-01-01

A microfluidic on-chip imaging cell sorter has several advantages over conventional cell sorting methods, especially to identify cells with complex morphologies such as clusters. One of the remaining problems is how to efficiently discriminate targets at the species level without labelling. Hence, we developed a label-free microfluidic droplet-sorting system based on image recognition of cells in droplets. To test the applicability of this method, a mixture of two plankton species with different morphologies (Dunaliella tertiolecta and Phaeodactylum tricornutum) were successfully identified and discriminated at a rate of 10 Hz. We also examined the ability to detect the number of objects encapsulated in a droplet. Single cell droplets sorted into collection channels showed 91 ± 4.5% and 90 ± 3.8% accuracy for D. tertiolecta and P. tricornutum, respectively. Because we used image recognition to confirm single cell droplets, we achieved highly accurate single cell sorting. The results indicate that the integrated method of droplet imaging cell sorting can provide a complementary sorting approach capable of isolating single target cells from a mixture of cells with high accuracy without any staining.

An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution.

PubMed

Girault, Mathias; Kim, Hyonchol; Arakawa, Hisayuki; Matsuura, Kenji; Odaka, Masao; Hattori, Akihiro; Terazono, Hideyuki; Yasuda, Kenji

2017-01-06

A microfluidic on-chip imaging cell sorter has several advantages over conventional cell sorting methods, especially to identify cells with complex morphologies such as clusters. One of the remaining problems is how to efficiently discriminate targets at the species level without labelling. Hence, we developed a label-free microfluidic droplet-sorting system based on image recognition of cells in droplets. To test the applicability of this method, a mixture of two plankton species with different morphologies (Dunaliella tertiolecta and Phaeodactylum tricornutum) were successfully identified and discriminated at a rate of 10 Hz. We also examined the ability to detect the number of objects encapsulated in a droplet. Single cell droplets sorted into collection channels showed 91 ± 4.5% and 90 ± 3.8% accuracy for D. tertiolecta and P. tricornutum, respectively. Because we used image recognition to confirm single cell droplets, we achieved highly accurate single cell sorting. The results indicate that the integrated method of droplet imaging cell sorting can provide a complementary sorting approach capable of isolating single target cells from a mixture of cells with high accuracy without any staining.

Automatic Spike Sorting Using Tuning Information

PubMed Central

Ventura, Valérie

2011-01-01

Current spike sorting methods focus on clustering neurons’ characteristic spike waveforms. The resulting spike-sorted data are typically used to estimate how covariates of interest modulate the firing rates of neurons. However, when these covariates do modulate the firing rates, they provide information about spikes’ identities, which thus far have been ignored for the purpose of spike sorting. This letter describes a novel approach to spike sorting, which incorporates both waveform information and tuning information obtained from the modulation of firing rates. Because it efficiently uses all the available information, this spike sorter yields lower spike misclassification rates than traditional automatic spike sorters. This theoretical result is verified empirically on several examples. The proposed method does not require additional assumptions; only its implementation is different. It essentially consists of performing spike sorting and tuning estimation simultaneously rather than sequentially, as is currently done. We used an expectation-maximization maximum likelihood algorithm to implement the new spike sorter. We present the general form of this algorithm and provide a detailed implementable version under the assumptions that neurons are independent and spike according to Poisson processes. Finally, we uncover a systematic flaw of spike sorting based on waveform information only. PMID:19548802

Automatic spike sorting using tuning information.

PubMed

Ventura, Valérie

2009-09-01

Current spike sorting methods focus on clustering neurons' characteristic spike waveforms. The resulting spike-sorted data are typically used to estimate how covariates of interest modulate the firing rates of neurons. However, when these covariates do modulate the firing rates, they provide information about spikes' identities, which thus far have been ignored for the purpose of spike sorting. This letter describes a novel approach to spike sorting, which incorporates both waveform information and tuning information obtained from the modulation of firing rates. Because it efficiently uses all the available information, this spike sorter yields lower spike misclassification rates than traditional automatic spike sorters. This theoretical result is verified empirically on several examples. The proposed method does not require additional assumptions; only its implementation is different. It essentially consists of performing spike sorting and tuning estimation simultaneously rather than sequentially, as is currently done. We used an expectation-maximization maximum likelihood algorithm to implement the new spike sorter. We present the general form of this algorithm and provide a detailed implementable version under the assumptions that neurons are independent and spike according to Poisson processes. Finally, we uncover a systematic flaw of spike sorting based on waveform information only.

Jab1 regulates Schwann cell proliferation and axonal sorting through p27

PubMed Central

Porrello, Emanuela; Rivellini, Cristina; Dina, Giorgia; Triolo, Daniela; Del Carro, Ubaldo; Ungaro, Daniela; Panattoni, Martina; Feltri, Maria Laura; Wrabetz, Lawrence; Pardi, Ruggero; Quattrini, Angelo

2014-01-01

Axonal sorting is a crucial event in nerve formation and requires proper Schwann cell proliferation, differentiation, and contact with axons. Any defect in axonal sorting results in dysmyelinating peripheral neuropathies. Evidence from mouse models shows that axonal sorting is regulated by laminin211– and, possibly, neuregulin 1 (Nrg1)–derived signals. However, how these signals are integrated in Schwann cells is largely unknown. We now report that the nuclear Jun activation domain–binding protein 1 (Jab1) may transduce laminin211 signals to regulate Schwann cell number and differentiation during axonal sorting. Mice with inactivation of Jab1 in Schwann cells develop a dysmyelinating neuropathy with axonal sorting defects. Loss of Jab1 increases p27 levels in Schwann cells, which causes defective cell cycle progression and aberrant differentiation. Genetic down-regulation of p27 levels in Jab1-null mice restores Schwann cell number, differentiation, and axonal sorting and rescues the dysmyelinating neuropathy. Thus, Jab1 constitutes a regulatory molecule that integrates laminin211 signals in Schwann cells to govern cell cycle, cell number, and differentiation. Finally, Jab1 may constitute a key molecule in the pathogenesis of dysmyelinating neuropathies. PMID:24344238

Feature extraction using extrema sampling of discrete derivatives for spike sorting in implantable upper-limb neural prostheses.

PubMed

Zamani, Majid; Demosthenous, Andreas

2014-07-01

Next generation neural interfaces for upper-limb (and other) prostheses aim to develop implantable interfaces for one or more nerves, each interface having many neural signal channels that work reliably in the stump without harming the nerves. To achieve real-time multi-channel processing it is important to integrate spike sorting on-chip to overcome limitations in transmission bandwidth. This requires computationally efficient algorithms for feature extraction and clustering suitable for low-power hardware implementation. This paper describes a new feature extraction method for real-time spike sorting based on extrema analysis (namely positive peaks and negative peaks) of spike shapes and their discrete derivatives at different frequency bands. Employing simulation across different datasets, the accuracy and computational complexity of the proposed method are assessed and compared with other methods. The average classification accuracy of the proposed method in conjunction with online sorting (O-Sort) is 91.6%, outperforming all the other methods tested with the O-Sort clustering algorithm. The proposed method offers a better tradeoff between classification error and computational complexity, making it a particularly strong choice for on-chip spike sorting.

A fully automated non-external marker 4D-CT sorting algorithm using a serial cine scanning protocol.

PubMed

Carnes, Greg; Gaede, Stewart; Yu, Edward; Van Dyk, Jake; Battista, Jerry; Lee, Ting-Yim

2009-04-07

Current 4D-CT methods require external marker data to retrospectively sort image data and generate CT volumes. In this work we develop an automated 4D-CT sorting algorithm that performs without the aid of data collected from an external respiratory surrogate. The sorting algorithm requires an overlapping cine scan protocol. The overlapping protocol provides a spatial link between couch positions. Beginning with a starting scan position, images from the adjacent scan position (which spatial match the starting scan position) are selected by maximizing the normalized cross correlation (NCC) of the images at the overlapping slice position. The process was continued by 'daisy chaining' all couch positions using the selected images until an entire 3D volume was produced. The algorithm produced 16 phase volumes to complete a 4D-CT dataset. Additional 4D-CT datasets were also produced using external marker amplitude and phase angle sorting methods. The image quality of the volumes produced by the different methods was quantified by calculating the mean difference of the sorted overlapping slices from adjacent couch positions. The NCC sorted images showed a significant decrease in the mean difference (p < 0.01) for the five patients.

Interval linear programming model for long-term planning of vehicle recycling in the Republic of Serbia under uncertainty.

PubMed

Simic, Vladimir; Dimitrijevic, Branka

2015-02-01

An interval linear programming approach is used to formulate and comprehensively test a model for optimal long-term planning of vehicle recycling in the Republic of Serbia. The proposed model is applied to a numerical case study: a 4-year planning horizon (2013-2016) is considered, three legislative cases and three scrap metal price trends are analysed, availability of final destinations for sorted waste flows is explored. Potential and applicability of the developed model are fully illustrated. Detailed insights on profitability and eco-efficiency of the projected contemporary equipped vehicle recycling factory are presented. The influences of the ordinance on the management of end-of-life vehicles in the Republic of Serbia on the vehicle hulks procuring, sorting generated material fractions, sorted waste allocation and sorted metals allocation decisions are thoroughly examined. The validity of the waste management strategy for the period 2010-2019 is tested. The formulated model can create optimal plans for procuring vehicle hulks, sorting generated material fractions, allocating sorted waste flows and allocating sorted metals. Obtained results are valuable for supporting the construction and/or modernisation process of a vehicle recycling system in the Republic of Serbia. © The Author(s) 2015.

An on-chip imaging droplet-sorting system: a real-time shape recognition method to screen target cells in droplets with single cell resolution

PubMed Central

Girault, Mathias; Kim, Hyonchol; Arakawa, Hisayuki; Matsuura, Kenji; Odaka, Masao; Hattori, Akihiro; Terazono, Hideyuki; Yasuda, Kenji

2017-01-01

A microfluidic on-chip imaging cell sorter has several advantages over conventional cell sorting methods, especially to identify cells with complex morphologies such as clusters. One of the remaining problems is how to efficiently discriminate targets at the species level without labelling. Hence, we developed a label-free microfluidic droplet-sorting system based on image recognition of cells in droplets. To test the applicability of this method, a mixture of two plankton species with different morphologies (Dunaliella tertiolecta and Phaeodactylum tricornutum) were successfully identified and discriminated at a rate of 10 Hz. We also examined the ability to detect the number of objects encapsulated in a droplet. Single cell droplets sorted into collection channels showed 91 ± 4.5% and 90 ± 3.8% accuracy for D. tertiolecta and P. tricornutum, respectively. Because we used image recognition to confirm single cell droplets, we achieved highly accurate single cell sorting. The results indicate that the integrated method of droplet imaging cell sorting can provide a complementary sorting approach capable of isolating single target cells from a mixture of cells with high accuracy without any staining. PMID:28059147

Is there a basin-centered gas accumulation in Cotton Valley Group Sandstones, Gulf Coast Basin, U.S.A.?

USGS Publications Warehouse

Bartberger, Charles E.; Dyman, Thaddeus S.; Condon, Steven M.

2002-01-01

The U.S. Geological Survey (USGS), in cooperation with the U.S. Department of Energy, is reevaluating the resource potential of selected domestic basin-centered gas accumulations. Basin-centered gas accumulations are characterized by presence of gas in extensive low-permeability (tight) reservoirs in which conventional seals and trapping mechanisms are absent, abnormally high or low reservoir pressures exist, and gas-water contacts are absent. In 1995, the USGS assessed one basin-centered gas play and two conventional plays within the trend of Jurassic and Cretaceous Cotton Valley Group fl uvial-deltaic and barrierisland/ strandplain sandstones across the onshore northern Gulf of Mexico Basin. Detailed evaluation of geologic and production data provides new insights into these Cotton Valley plays. Two Cotton Valley sandstone trends are identifi ed based on reservoir properties and gas-production characteristics. Transgressive blanket sandstones across northern Louisiana have relatively high porosity and permeability and do not require fracture stimulation to produce gas at commercial rates. South of this trend, and extending westward into eastern Texas, massive sandstones of the Cotton Valley trend exhibit low porosity and permeability and require fracture stimulation. The high permeability of Cotton Valley blanket sandstones is not conducive to the presence of basin-centered gas, but lowpermeability massive sandstones provide the type of reservoir in which basin-centered gas accumulations commonly occur. Data on source rocks, including burial and thermal history, are consistent with the interpretation of potential basincentered gas within Cotton Valley sandstones. However, pressure gradients throughout most of the blanket- and massivesandstone trends are normal or nearly normal, which is not characteristic of basin-centered gas accumulations. The presence of gas-water contacts in at least seven fi elds across the blanket-sandstone trend together with relatively high permeabilities and high gas-production rates without fracture stimulation indicate that fi elds in this trend are conventional. Within the tight massive-sandstone trend, permeability is suffi ciently low that gas-water transition zones are vertically extensive and gas-water contacts either have not been encountered or are poorly defi ned. With increasing depth through these transition zones, gas saturation decreases and water saturation increases until eventually gas saturations become suffi ciently low that, in terms of ultimate cumulative production, wells are noncommercial. Such progressive increase in water saturation with depth suggests that poorly defi ned gas-water contacts probably are present below the depth at which wells become noncommercial. The interpreted presence of gas-water contacts within the tight, Cotton Valley massive-sandstone trend suggests that gas accumulations in this trend, too, are conventional, and that a basin-centered gas accumulation does not exist within Cotton Valley sandstones in the northern Gulf Basin.

Clinical investigation of set-shifting subtypes in anorexia nervosa.

PubMed

Abbate-Daga, Giovanni; Buzzichelli, Sara; Marzola, Enrica; Amianto, Federico; Fassino, Secondo

2014-11-30

While evidence continues to accumulate on the relevance of cognitive inflexibility in anorexia nervosa (AN), its clinical correlates remain unclear. We aimed at examining the relationship between set-shifting and clinical variables (i.e., eating psychopathology, depression, and personality) in AN. Ninety-four individuals affected by AN and 59 healthy controls (HC) were recruited. All participants were assessed using: Eating Disorders Inventory-2 (EDI-2), Temperament and Character Inventory (TCI), Beck Depression Inventory (BDI), and Wisconsin Card Sorting Test (WCST). The AN group scored worse than HCs on set-shifting. According to their neuropsychological performances, AN patients were split into two groups corresponding to poor (N=30) and intact (N=64) set-shifting subtypes. Interoceptive awareness, impulse regulation, and maturity fears on the EDI-2 and depression on the BDI differed across all groups (HC, intact, and poor set-shifting subtype). Self-directedness on the TCI differed significantly among all groups. Cooperativeness and reward dependence differed instead only between HC and AN poor set-shifting subtype. After controlling for depression, only interoceptive awareness remained significant with reward dependence showing a trend towards statistical significance. These findings suggest that multiple clinical variables may be correlated with set-shifting performances in AN. The factors contributing to impaired cognitive inflexibility could be more complex than heretofore generally considered. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

An improved infrared technique for sorting pecans

NASA Astrophysics Data System (ADS)

Graeve, Thorsten; Dereniak, Eustace L.; Lamonica, John A., Jr.

1991-10-01

This paper presents the results of a study of pecan spectral reflectances. It describes an experiment for measuring the contrast between several components of raw pecan product to be sorted. An analysis of the experimental data reveals high contrast ratios in the infrared spectrum, suggesting a potential improvement in sorting efficiency when separating pecan meat from shells. It is believed that this technique has the potential to dramatically improve the efficiency of current sorting machinery, and to reduce the cost of processing pecans for the consumer market.

[THE PRINCIPLES OF ORGANIZATION AND TREATMENT FOR SORTING OF WOUNDED PERSONS WITH A COMBAT SURGICAL TRAUMA OF EXTREMITIES ON THE IV LEVEL OF THE MEDICAL CARE PROVISION].

PubMed

Korohl, S O; Zherdev, I I; Domanskiy, A M

2015-12-01

Experience of medical sorting of 434 injured persons with a gun-shot woundings of extremities in 2014-2015 yrs is adduced. The principles of organization and treatment for medical sorting of wounded persons were elaborated. Prognostic intrahospital, diagnostic and evacuation--transport sorting was introduced in wounded persons in the IV level hospital, concerning severity of traumatic shock and prognosis of their survival.

How I Learned to Stop Worrying and Love Eclipsing Binaries

NASA Astrophysics Data System (ADS)

Moe, Maxwell Cassady

Relatively massive B-type stars with closely orbiting stellar companions can evolve to produce Type Ia supernovae, X-ray binaries, millisecond pulsars, mergers of neutron stars, gamma ray bursts, and sources of gravitational waves. However, the formation mechanism, intrinsic frequency, and evolutionary processes of B-type binaries are poorly understood. As of 2012, the binary statistics of massive stars had not been measured at low metallicities, extreme mass ratios, or intermediate orbital periods. This thesis utilizes large data sets of eclipsing binaries to measure the physical properties of B-type binaries in these previously unexplored portions of the parameter space. The updated binary statistics provide invaluable insight into the formation of massive stars and binaries as well as reliable initial conditions for population synthesis studies of binary star evolution. We first compare the properties of B-type eclipsing binaries in our Milky Way Galaxy and the nearby Magellanic Cloud Galaxies. We model the eclipsing binary light curves and perform detailed Monte Carlo simulations to recover the intrinsic properties and distributions of the close binary population. We find the frequency, period distribution, and mass-ratio distribution of close B-type binaries do not significantly depend on metallicity or environment. These results indicate the formation of massive binaries are relatively insensitive to their chemical abundances or immediate surroundings. Second, we search for low-mass eclipsing companions to massive B-type stars in the Large Magellanic Cloud Galaxy. In addition to finding such extreme mass-ratio binaries, we serendipitously discover a new class of eclipsing binaries. Each system comprises a massive B-type star that is fully formed and a nascent low-mass companion that is still contracting toward its normal phase of evolution. The large low-mass secondaries discernibly reflect much of the light they intercept from the hot B-type stars, thereby producing sinusoidal variations in perceived brightness as they orbit. These nascent eclipsing binaries are embedded in the hearts of star-forming emission nebulae, and therefore provide a unique snapshot into the formation and evolution of massive binaries and stellar nurseries. We next examine a large sample of B-type eclipsing binaries with intermediate orbital periods. To achieve such a task, we develop an automated pipeline to classify the eclipsing binaries, measure their physical properties from the observed light curves, and recover the intrinsic binary statistics by correcting for selection effects. We find the population of massive binaries at intermediate separations differ from those orbiting in close proximity. Close massive binaries favor small eccentricities and have correlated component masses, demonstrating they coevolved via competitive accretion during their formation in the circumbinary disk. Meanwhile, B-type binaries at slightly wider separations are born with large eccentricities and are weighted toward extreme mass ratios, indicating the components formed relatively independently and subsequently evolved to their current configurations via dynamical interactions. By using eclipsing binaries as accurate age indicators, we also reveal that the binary orbital eccentricities and the line-of-sight dust extinctions are anticorrelated with respect to time. These empirical relations provide robust constraints for tidal evolution in massive binaries and the evolution of the dust content in their surrounding environments. Finally, we compile observations of early-type binaries identified via spectroscopy, eclipses, long-baseline interferometry, adaptive optics, lucky imaging, high-contrast photometry, and common proper motion. We combine the samples from the various surveys and correct for their respective selection effects to determine a comprehensive nature of the intrinsic binary statistics of massive stars. We find the probability distributions of primary mass, secondary mass, orbital period, and orbital eccentricity are all interrelated. These updated multiplicity statistics imply a greater frequency of low-mass X-ray binaries, millisecond pulsars, and Type Ia supernovae than previously predicted.

Hydrostratigraphic mapping of the Milford-Souhegan glacial drift aquifer, and effects of hydrostratigraphy on transport of PCE, Operable Unit 1, Savage Superfund Site, Milford, New Hampshire

USGS Publications Warehouse

Harte, Philip T.

2010-01-01

The Savage Municipal Well Superfund site in the Town of Milford, New Hampshire, was underlain by a 0.5-square mile plume (as mapped in 1994) of volatile organic compounds (VOCs), most of which consisted of tetrachloroethylene (PCE). The plume occurs mostly within highly transmissive stratified-drift deposits but also extends into underlying till and bedrock. The plume has been divided into two areas called Operable Unit 1 (OU1), which contains the primary source area, and Operable Unit 2 (OU2), which is defined as the extended plume area outside of OU1. The OU1 remedial system includes a low-permeability barrier wall that encircles the highest detected concentrations of PCE and a series of injection and extraction wells to contain and remove contaminants. The barrier wall likely penetrates the full thickness of the sand and gravel; in many places, it also penetrates the full thickness of the underlying basal till and sits atop bedrock.From 1998 to 2004, PCE concentrations decreased by an average of 80 percent at most wells outside the barrier wall. However, inside the barrier, PCE concentrations greater than 10,000 micrograms per liter (μg/L) still exist (2008). The remediation of these areas of recalcitrant PCE presents challenges to successful remediation.The U.S. Geological Survey (USGS), in cooperation with the New Hampshire Department of Environmental Services (NHDES) and the U.S. Environmental Protection Agency (USEPA), Region 1, is studying the solute transport of VOCs (primarily PCE) in contaminated groundwater in the unconsolidated sediments (overburden) of the Savage site and specifically assisting in the evaluation of the effectiveness of remedial operations in the OU1 area. As part of this effort, the USGS analyzed the subsurface stratigraphy to help understand hydrostratigraphic controls on remediation.A combination of lithologic, borehole natural gamma-ray and electromagnetic (EM) induction logging, and test drilling has identified 11 primary hydrostratigraphic units in OU1. These 11 units consist of several well-sorted sandy layers with some gravel that are separated by poorly sorted cobble layers with a fine-grained matrix. Collectively these units represent glacial sediments deposited by localized ice-margin fluctuations. For the most part, the units are semi-planar, particularly the cobble units, and truncated by an undulating bedrock surface. The lowermost unit is a basal till that ranges in thickness from zero to greater than 10 feet and mantles the bedrock surface.The 11 units have different lithologic and hydraulic characteristics. The hydraulic conductivity of the well-sorted sand and gravel units is typically greater than the conductivity of the poorly sorted cobble units and the basal till. The hydraulic conductivity ranges from 5 to greater than 500 feet per day. Lateral and vertical variation in lithology and hydraulic conductivity are inferred by variations in borehole natural gamma-ray counts and estimates of hydraulic conductivity.The comparison of hydrostratigraphic units with the spatial distribution of PCE concentrations suggests that solute transport away from source areas is primarily lateral within the permeable sandy units in the middle to lower parts of the aquifer. Along the centerline of the interior barrier area, highest PCE concentrations are in the sandy units to the east of suspected source areas.

Sorted bed forms as self-organized patterns: 2. complex forcing scenarios

USGS Publications Warehouse

Coco, Giovanni; Murray, A. Brad; Green, Malcom O.; Thieler, E. Robert; Hume, T.M.

2007-01-01

We employ a numerical model to study the development of sorted bed forms under a variety of hydrodynamic and sedimentary conditions. Results indicate that increased variability in wave height decreases the growth rate of the features and can potentially give rise to complicated, a priori unpredictable, behavior. This happens because the system responds to a change in wave characteristics by attempting to self-organize into a patterned seabed of different geometry and spacing. The new wavelength might not have enough time to emerge before a new change in wave characteristics occurs, leading to less regular seabed configurations. The new seabed configuration is also highly dependent on the preexisting morphology, which further limits the possibility of predicting future behavior. For the same reasons, variability in the mean current magnitude and direction slows down the growth of features and causes patterns to develop that differ from classical sorted bed forms. Spatial variability in grain size distribution and different types of net sediment aggradation/degradation can also result in the development of sorted bed forms characterized by a less regular shape. Numerical simulations qualitatively agree with observed geometry (spacing and height) of sorted bed forms. Also in agreement with observations is that at shallower depths, sorted bed forms are more likely to be affected by changes in the forcing conditions, which might also explain why, in shallow waters, sorted bed forms are described as ephemeral features. Finally, simulations indicate that the different sorted bed form shapes and patterns observed in the field might not necessarily be related to diverse physical mechanisms. Instead, variations in sorted bed form characteristics may result from variations in local hydrodynamic and/or sedimentary conditions.