Science.gov

Sample records for postradavshemu ot katastrofy

  1. Master: 2 new OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Buckley, D.; Tiurina, N.; Gorbovskoy, E.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Gress, O.; Vladimirov, V.; Kuvshinov, D.; Tlatov, A.; Senik, V.; Dormidontov, D.; Gabovich, A.; Pogrosheva, T.

    2017-01-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 58m 24.93s -69d 44m 51.3s on 2017-01-25.02720 UT. The OT unfiltered magnitude is 18.0m (mlim=19.0).

  2. MASTER: PSN and new OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gorbovskoy, E.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Budnev, N.; Gress, O.; Ivanov, K.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Chazov, V.; Shumkov, V.; Pogrosheva, T.; Postnikov, L.; Nikitin, V.; Kuvshinov, D.

    2017-01-01

    MASTER-Tunka auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 07h 50m 43.35s +79d 09m 16.6s on 2017-01-23.88841 UT. The OT unfiltered magnitude is 17.8m (limit 19.6m).

  3. MASTER: QSO flare and OTs

    NASA Astrophysics Data System (ADS)

    Gress, O.; Shumkov, V.; Pogrosheva, T.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Ivanov, K.; Vladimirov, V.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-OAFA auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 01h 50m 52.97s -45d 10m 15.9s on 2016-10-06.21896 UT with unfiltered m_OT=17.4m (limit 20.6m).

  4. Time comparison via OTS-2

    NASA Technical Reports Server (NTRS)

    Dejong, G.; Kaarls, R.; Kirchner, D.; Ressler, H.

    1982-01-01

    The time comparisons carried out via OTS-2 between the Technical University Graz (Austria) and the Van Swinden Laboratory Delft (Netherlands) are discussed. The method is based on the use of the synchronization pulse in the TV-frame of the daily evening broadcasting of a French TV-program to Northern Africa. Corrections, as a consequence of changes in the position of the satellite coordinates are applied weekly after reception of satellite coordinates. A description of the method is given as well as some of the particular techniques used in both the participating laboratories. Preliminary results are presented.

  5. MASTER-OAFA discovery: new bright OT

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Balanutsa, P.; Pogrosheva, T.; Lipunov, V.; Podesta, R.; Levato, H.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Lopez, C.; Podesta, F.; Saffe, C.; Potter, S.

    2016-10-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 06m 06.62s -62d 13m 31.3s on 2016-10-19.03627 UT. The OT unfiltered (limit 19.1m).

  6. MASTER-OAFA: OT detection during inspection

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Chazov, V.; Gorbunov, I.; Krylov, A.; Lopez, C.; Podesta, F.; Saffe, C.; Shumkov, V.; Pogrosheva, T.

    2017-02-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 36m 27.60s -47d 35m 18.1s on 2017-02-02.36706 UT. The OT unfiltered magnitude is 16.2m (mlim=18.1m).

  7. MASTER: new OT and QSO flare

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Buckley, D.; Budnev, N.; Gress, O.; Gorbovskoy, E.; Tiurina, N.; Vladimirov, V.; Kornilov, V.; Kuvshinov, D.; Pogrosheva, T.; Ivanov, K.

    2017-02-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 09h 45m 05.87s -65d 43m 43.3s on 2017-02-15.91771 UT. The OT unfiltered magnitude is (mlim=18.6).

  8. MASTER: 2 OT discovered in Argentina

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Pogrosheva, T.; Lipunov, V.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Vladimirov, V.; Gress, O.; Ivanov, K.; Chazov, V.; Lopez, C.; Podesta, F.; Saffe, C.

    2016-10-01

    MASTER-OAFA, located in Argentina, with auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L) discovered OT source at (RA, Dec) = 03h 19m 42.92s -45d 30m 13.9s on 2016-10-27.27597 UT. The OT unfiltered magnitude is 16.9m (mlim=20.8m).

  9. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... process requests for unpublished OTS information and, where appropriate, for the OTS to assert evidentiary... have been or might be released, or the testimony of other non-OTS persons, including retained experts. (ii) OTS employees will not be authorized to provide expert or opinion testimony for private...

  10. MASTER: OT detection during Fermi trigger inspection

    NASA Astrophysics Data System (ADS)

    Popova, E.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Potter, S.; Gabovich, A.

    2016-11-01

    During inspection of Fermi trigger 501261070 ( (Ra,Dec)=47.190,-47.210; GRB_ERROR_radius=3.27deg, GRB_TIME=2016/11/19 15:11:06.40UT http://gcn.gsfc.nasa.gov/other/501261070.fermi ) MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 22m 52.70s -48d 29m 10.9s on 2016-11-19 21:17:17.878UT with unfiltered m_OT=17.8 (mlim=19.7).

  11. CNEOST OT PTSS-17dmk, discovery - new OT, no VIZIER data

    NASA Astrophysics Data System (ADS)

    Li, Bin; Zhao, Haibin; Wang, Xiaofeng; Li, Wenxiong; Xu, Zhijian; Tan, Hanjie; Wang, Lifan; Zhang, Jujia

    2017-03-01

    PTSS (Purple Mountain Observtory(PMO) & Tsinghua Supernova Survey ) discovered OT source PTSS-17jdx at (RA, Dec) = 13h20m53.59s -04d14m04.70s on 2017-02-07.727524 UT with the 1.04/1.8 Schmidt Telescope( named Chinese Near Earth Object Survey Telescope , CNEOST ) at Xuyi station of PMO. The OT PTSS-17jdx SDSS-r magnitude is 17.5m.

  12. CNEOST OT PTSS-17dmk, discovery - new OT, no VIZIER data

    NASA Astrophysics Data System (ADS)

    Li, Bin; Zhao, Haibin; Wang, Lifan; Xu, Zhijian; Li, Wenxiong; Wang, Xiaofeng; Tan, Hanjie; Rui, Liming; Yang, Zesheng

    2017-02-01

    PTSS (Purple Mountain Observtory(PMO) & Tsinghua Supernova Survey ) discovered OT source at (RA, Dec) = 05h 28m 21.88s +59d 38m 39.26s on 2017-02-09.557376 UT with the 1.04/1.8 Schmidt Telescope( named Chinese Near Earth Object Survey Telescope , CNEOST http://www.cneost.org ) at Xuyi station of PMO. The OT (PTSS-17dmk) SDSS-r magnitude is 17.2m (limit 18.7m).

  13. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... information also includes information that current and former employees, officers, and agents obtained in... a current or former employee, officer, or agent of the OTS (or the Federal Home Loan Bank Board, the... requests that current or former OTS employees be authorized to give testimony. (i) The request...

  14. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... information also includes information that current and former employees, officers, and agents obtained in... a current or former employee, officer, or agent of the OTS (or the Federal Home Loan Bank Board, the... requests that current or former OTS employees be authorized to give testimony. (i) The request...

  15. MASTER-OAFA, MASTER-SAAO discovery: bright OTs

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Shumkov, V.; Popova, E.; Lipunov, V.; Podesta, R.; Levato, H.; Buckley, D.; Lopez, C.; Podesta, F.; Saffe, C.; Potter, S.; Gorbovskoy, E.; Balanutsa, P.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Gress, O.; Chazov, V.; Ivanov, K.

    2016-11-01

    MASTER-OAFA (located in Argentina) auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 20h 26m 36.17s -44d 07m 22.7s on 2016-11-29.04023 UT. The OT unfiltered magnitude is (mlim=18.6m).

  16. Fast bright OT in PGC2697102 discovered by MASTER

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Tlatov, A.; Gorbovskoy, E.; Chazov, V.; Tiurina, N.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Vladimirov, V.; Gress, O.; Ivanov, K.; Kuvshinov, D.; Senik, V.; Dormidontov, D.; Parkhomenko, A.; Gabovich, A.

    2016-11-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 16h 15m 32.60s +67d 14m 45.8s on 2016-11-10.73375 UT. The OT unfiltered magnitude is (mlim=18.7m).

  17. Master-Saao Psn in PGC152788 and OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Gress, O.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Chazov, V.; Kuvshinov, D.; Pogrosheva, T.; Shumkov, V.

    2017-02-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 08h 32m 56.92s -03d 51m 28.1s on 2017-02-11 22:13:06.767UT . The OT unfiltered magnitude is (mlim=19.8).

  18. MASTER-SAAO: OT detected during Fermi 508270281 inspection

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Gress, O.; Shumkov, V.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Potter, S.

    2017-02-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 11h 34m 18.80s -51d 33m 13.5s on 2017-02-08.93060 UT. The OT unfiltered magnitude is (mlim=18.4).

  19. 12 CFR 500.10 - The OTS or The Office.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS General Organization § 500.10 The OTS or The Office. The Office of Thrift Supervision (referred...

  20. 75 FR 51169 - OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... Office of Thrift Supervision OTS Minority Depository Institutions Advisory Committee AGENCY: Department... Minority Depository Institutions Advisory Committee will renew for a two-year period beginning August 2...), and with the approval of the Secretary of the Treasury to announce the renewal of the OTS...

  1. 76 FR 7630 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee... OTS Minority Depository Institutions Advisory Committee (MDIAC) will convene a meeting on Tuesday..., the Office of Thrift Supervision is announcing that the OTS Minority Depository Institutions...

  2. 75 FR 13344 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ... Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee... OTS Minority Depository Institutions Advisory Committee (MDIAC) will convene a meeting on Wednesday... Supervision is announcing that the OTS Minority Depository Institutions Advisory Committee will convene...

  3. MASTER-IAC: very bright high amplitude OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Lodieu, N.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Vladimirov, V.; Kornilov, V.; Chazov, V.; Kuvshinov, D.; Gress, O.; Budnev, N.; Ivanov, K.

    2016-12-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 02m 05.67s +25d 48m 34.3s on 2016-12-04.96045 UT. The OT unfiltered magnitude is 13.7m (m_limit=20.3m).

  4. MASTER: OT discovered during inspection of HESE 58537957 trigger

    NASA Astrophysics Data System (ADS)

    Tyurina, N.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Kuvshinov, D.; Vlasenko, D.; Gress, O.; Ivanov, K.; Shumkov, V.; Potter, S.

    2016-08-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 13h 08m 45.02s -32d 32m 54.9s on 2016-08-24.73811 UT during inspection of HESE alert ( 58537957 trigger number ) http://gcn.gsfc.nasa.gov/notices_amon/58537957_128340.amon . The OT unfiltered magnitude is 19.6m (limit 20.5m).

  5. 2 new OT, discovered by MASTER-OAFA (Argentina)

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Vladimirov, V.; Balanutsa, P.; Kornilov, V.; Gress, O.; Chazov, V.; Kuvshinov, D.; Lopez, C.; Podesta, F.; Saffe, C.; Gabovich, A.

    2016-11-01

    MASTER-OAFA (located in Argentina) auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 09h 23m 26.72s -76d 28m 17.7s on 2016-11-13 06:19:38UT The OT magnitude in unfiltered is (mlim=18.0m).

  6. OT promotes closer interpersonal distance among highly empathic individuals.

    PubMed

    Perry, Anat; Mankuta, David; Shamay-Tsoory, Simone G

    2015-01-01

    The space between people, or 'interpersonal distance', creates and defines the dynamics of social interactions and is a salient cue signaling responsiveness and feeling comfortable. This distance is implicit yet clearly felt, especially if someone stands closer or farther away than expected. Increasing evidence suggests that Oxytocin (OT) serves as a social hormone in humans, and that one of its roles may be to alter the perceptual salience of social cues. Considering that empathic ability may shape the way individuals process social stimuli, we predicted that OT will differentially affect preferred interpersonal distance depending on individual differences in empathy. Participants took part in two interpersonal distance experiments: In the first, they had to stop a (computer visualized) protagonist when feeling most comfortable; in the second, they were asked to choose the room in which they would later discuss intimate topics with another. Both experiments revealed an interaction between the effect of OT and empathy level. Among highly empathic individuals, OT promoted the choice of closer interpersonal distances. Yet, OT had an opposite effect on individuals with low empathic traits. We conclude that the enhancement of social cues following OT administration may have opposite effects on individuals with different empathic abilities.

  7. A new assessment method of outdoor tobacco smoke (OTS) exposure

    NASA Astrophysics Data System (ADS)

    Cho, Hyeri; Lee, Kiyoung

    2014-04-01

    Outdoor tobacco smoke (OTS) is concerned due to potential health effects. An assessment method of OTS exposure is needed to determine effects of OTS and validate outdoor smoking policies. The objective of this study was to develop a new method to assess OTS exposure. This study was conducted at 100 bus stops including 50 centerline bus stops and 50 roadside bus stops in Seoul, Korea. Using real-time aerosol monitor, PM2.5 was measured for 30 min at each bus stop in two seasons. ‘Peak analysis' method was developed to assess short term PM2.5 exposure by OTS. The 30-min average PM2.5 exposure at each bus stop was associated with season and bus stop location but not smoking activity. The PM2.5 peak occurrence rate by the peak analysis method was significantly associated with season, bus stop location, observed smoking occurrence, and the number of buses servicing a route. The PM2.5 peak concentration was significantly associated with season, smoking occurrence, and the number of buses servicing a route. When a smoker was standing still at the bus stop, magnitude of peak concentrations were significantly higher than when the smoker walking-through the bus stop. People were exposed to high short-term PM2.5 peak levels at bus stops, and the magnitude of peak concentrations were highest when a smoker was located close to the monitor. The magnitude of peak concentration was a good indicator helped distinguish nearby OTS exposure. Further research using ‘peak analysis' is needed to measure smoking-related exposure to PM2.5 in other outdoor locations.

  8. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Section 510.5 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY MISCELLANEOUS... with the OTS's performance of its responsibilities, such as records concerning supervision, regulation... authority having general supervision of such state-chartered savings associations. (iv) Reports...

  9. Counterpart of MASTER OT J084140.94+722732.6

    NASA Astrophysics Data System (ADS)

    Nesci, Roberto

    2016-02-01

    Following the ATel #8725 by Balanutsa et al. about MASTER OT J084140.94+722732.6, I made aperture photometry on all the Palomar survey plates (12) available from the MAST archive to get an indication of the star color.

  10. 75 FR 24775 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-05

    ... interested in listening to the meeting and members of the public who require auxiliary aid should e-mail OTS... the MSAAC by any one of the following methods: E-mail address: mutualcommittee@ots.treas.gov ; or...

  11. Software OT&E Guidelines. Volume 1. Software Test Manager’s Handbook

    DTIC Science & Technology

    1981-02-01

    CLASSIFICATION OF THIS PAGE( W ~h Data Xntetd) Blk 20 cont’d. (AFTECP 800-4); 5. Software Support Facility Evaluation Tools User’s Handbook (AFTECP 800...description of the AFTEC approach to OT&E of software. The document is not directive, but rather a reference document for planning OT&E. It contains...Critical Design Review (CDR) ........... .... 39 M. AFTEC Produced Publications ............... ... 40 1. OT&E Approach . ..... .......... 41 2. OT&E

  12. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How will OTS review my business plan? 563b.115... business plan? (a) OTS will review your business plan to determine that it demonstrates a safe and sound... will be determinative. OTS will review every case on its merits. (b) You must file your business...

  13. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false How will OTS review my business plan? 563b.115... business plan? (a) OTS will review your business plan to determine that it demonstrates a safe and sound... will be determinative. OTS will review every case on its merits. (b) You must file your business...

  14. Teaching the Moving Child: OT Insights That Will Transform Your K-3 Classroom

    ERIC Educational Resources Information Center

    Berkey, Sybil M.

    2009-01-01

    Because sensorimotor and environmental factors have a profound effect on children's learning, every teacher should know how to weave strategies from occupational therapy (OT) into their everyday instruction. This is the guidebook K-3 teachers need to "think like an OT"--and form effective partnerships with OTs in their schools--so all students can…

  15. 75 FR 63895 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ... Office of Thrift Supervision Open Meeting of the OTS Minority Depository Institutions Advisory Committee... OTS Minority Depository Institutions Advisory Committee (MDIAC) will convene a meeting on Wednesday.... SUPPLEMENTARY INFORMATION: By this notice, the Office of Thrift Supervision is announcing that the OTS...

  16. MASTER OT J125532.84-050442.8 is Himalia

    NASA Astrophysics Data System (ADS)

    Denisenko, D.

    2016-11-01

    Bright optical transient MASTER OT J125532.84-050442.8 reported by Shumkov et al., ATel #9766 is the satellite of Jupiter Himalia. Here is the ephemeris of Jupiter VI for observatory code [Z22] around the reported time of observations by MASTER-IAC (2016-11-17.26843 UT = 06:26:32 UT) generated using MPC Natural Satellites Ephemeris Service: Date UT R.A. (J2000) Decl.

  17. Formose reaction accelerated in aerosol-OT reverse micelles

    PubMed Central

    Masaoka, Makoto; Michitaka, Tomohiro

    2016-01-01

    The formose reaction in reverse micelles of aerosol-OT (AOT), triton X-100 (TX), and hexadecyltrimethylammonium bromide (CTAB) was investigated. Time–conversion data have indicated that the interfacial water layer of AOT reverse micelles is a medium that accelerates formation of glycolaldehyde in the formose reaction. The 13C NMR spectra for the products of the formose reaction using formaldehyde-13C as starting material are indicative of the formation of ethylene glycol as a major product. PMID:28144336

  18. Optimization of OT-MACH Filter Generation for Target Recognition

    NASA Technical Reports Server (NTRS)

    Johnson, Oliver C.; Edens, Weston; Lu, Thomas T.; Chao, Tien-Hsin

    2009-01-01

    An automatic Optimum Trade-off Maximum Average Correlation Height (OT-MACH) filter generator for use in a gray-scale optical correlator (GOC) has been developed for improved target detection at JPL. While the OT-MACH filter has been shown to be an optimal filter for target detection, actually solving for the optimum is too computationally intensive for multiple targets. Instead, an adaptive step gradient descent method was tested to iteratively optimize the three OT-MACH parameters, alpha, beta, and gamma. The feedback for the gradient descent method was a composite of the performance measures, correlation peak height and peak to side lobe ratio. The automated method generated and tested multiple filters in order to approach the optimal filter quicker and more reliably than the current manual method. Initial usage and testing has shown preliminary success at finding an approximation of the optimal filter, in terms of alpha, beta, gamma values. This corresponded to a substantial improvement in detection performance where the true positive rate increased for the same average false positives per image.

  19. Complex architecture and regulated expression of the Sox2ot locus during vertebrate development

    PubMed Central

    Amaral, Paulo P.; Neyt, Christine; Wilkins, Simon J.; Askarian-Amiri, Marjan E.; Sunkin, Susan M.; Perkins, Andrew C.; Mattick, John S.

    2009-01-01

    The Sox2 gene is a key regulator of pluripotency embedded within an intron of a long noncoding RNA (ncRNA), termed Sox2 overlapping transcript (Sox2ot), which is transcribed in the same orientation. However, this ncRNA remains uncharacterized. Here we show that Sox2ot has multiple transcription start sites associated with genomic features that indicate regulated expression, including highly conserved elements (HCEs) and chromatin marks characteristic of gene promoters. To identify biological processes in which Sox2ot may be involved, we analyzed its expression in several developmental systems, compared to expression of Sox2. We show that Sox2ot is a stable transcript expressed in mouse embryonic stem cells, which, like Sox2, is down-regulated upon induction of embryoid body (EB) differentiation. However, in contrast to Sox2, Sox2ot is up-regulated during EB mesoderm-lineage differentiation. In adult mouse, Sox2ot isoforms were detected in tissues where Sox2 is expressed, as well as in different tissues, supporting independent regulation of expression of the ncRNA. Sox2dot, an isoform of Sox2ot transcribed from a distal HCE located >500 kb upstream of Sox2, was detected exclusively in the mouse brain, with enrichment in regions of adult neurogenesis. In addition, Sox2ot isoforms are transcribed from HCEs upstream of Sox2 in other vertebrates, including in several regions of the human brain. We also show that Sox2ot is dynamically regulated during chicken and zebrafish embryogenesis, consistently associated with central nervous system structures. These observations provide insight into the structure and regulation of the Sox2ot gene, and suggest conserved roles for Sox2ot orthologs during vertebrate development. PMID:19767420

  20. Monitoring the activity of the Be star OT Geminorum

    NASA Astrophysics Data System (ADS)

    Arellano Ferro, A.; Sareyan, J. P.; Avila, J. J.; Gonzalez, F.; Dumont, M.; Geos

    1998-02-01

    Observations obtained in 1995-1996 of the Be star OT Geminorum are reported and show that in october 1995 the star reached a very active phase with large variations around a bright plateau, a phase of activity we have also distinguished in previous observations dating from 1960. The time scales involved are discussed and suggest that the violent variations of the activity propagate very quickly on the whole surface of the star or on a huge portion of it. No likely pulsation periods were found. The assumption that the detected activity is due to the effects of an hypothetical companion leads to conclude that such companion could not be detected by the present interferometric techniques. Partly based on observations obtained at the La Luz Observatory of the University of Guanajuato, Mexico.

  1. Swift observations of MASTER OT J120451.50+265946.6 (Type Ib SN)

    NASA Astrophysics Data System (ADS)

    Margutti, Raffaella; Brown, Peter J.; Kamble, Atish; Milisavljevic, Dan; Parrent, Jerod; Soderberg, Alicia M.

    2014-11-01

    MASTER OT J120451.50+265946.6 (Atel #6634) has been recently classified as a Type Ib SN a few weeks after maximum light (Atel #6639, #6640, #6641). MASTER OT J120451.50+265946.6 exploded in NGC 4080 (distance of 15 Mpc, Karachentsev et al., 2013).

  2. 12 CFR 502.15 - How does OTS determine my size component?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true How does OTS determine my size component? 502.15... size component? (a) Chart. If you are a savings association, OTS uses the following chart to calculate your size component: If your total assets are: . . . Over—* Column A But not over— Column B Your...

  3. 12 CFR 502.15 - How does OTS determine my size component?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true How does OTS determine my size component? 502.15... size component? (a) Chart. If you are a savings association, OTS uses the following chart to calculate your size component: If your total assets are: . . . Over—* Column A But not over— Column B Your...

  4. 12 CFR 502.15 - How does OTS determine my size component?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false How does OTS determine my size component? 502... determine my size component? (a) Chart. If you are a savings association, OTS uses the following chart to calculate your size component: If your total assets are: . . . Over—* Column A But not over— Column B...

  5. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 6 2014-01-01 2012-01-01 true When will OTS adjust, add, waive, or eliminate a... ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive, or eliminate a fee....

  6. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  7. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  8. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false When will OTS adjust, add, waive, or eliminate... TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive,...

  9. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 6 2013-01-01 2012-01-01 true When will OTS adjust, add, waive, or eliminate a... ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under unusual circumstances, the Director may deem it necessary or appropriate to adjust, add, waive, or eliminate a fee....

  10. 75 FR 61572 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... interested in attending the meeting and members of the public who require auxiliary aid should e-mail OTS at.... The public is invited to submit written statements to the MSAAC by any one of the following methods: E-mail address: mutualcommittee@ots.treas.gov ; or Mail: to Charlotte Bahin, Designated Federal...

  11. 12 CFR 563.146 - Will the OTS permit my capital distribution?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... under 12 U.S.C. 1831o(d)(1)(B). (b) Your proposed capital distribution raises safety or soundness... 12 Banks and Banking 6 2012-01-01 2012-01-01 false Will the OTS permit my capital distribution... distribution? The OTS will review your notice or application under the review procedures in 12 CFR part...

  12. MASTER-Kislovodsk: bright OT (PSN or CV, ampl>5.2m)

    NASA Astrophysics Data System (ADS)

    Kuznetsov, N.; Shumkov, V.; Lipunov, V.; Tlatov, A.; Rebolo, R.; Serra-Ricart, M.; Senik, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Vlasenko, D.; Dormidontov, D.; Parkhomenko, A.; Gress, O.; Ivanov, K.; Budnev, N.

    2016-10-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 10h 06m 29.13s +22d 26m 43.8s with unfiltered m_OT=16.8m on 2016-10-09 00:56:53.746UT.

  13. MASTER: QSO flare (5BZQ J1312-0424) and OTs

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Shumkov, V.; Pogrosheva, T.; Popova, E.; Lipunov, V.; Podesta, R.; Levato, H.; Lopez, C.; Podesta, F.; Tlatov, A.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Ivanov, O. Gress K.; Chazov, V.

    2016-11-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 13h 12m 50.91s -04d 24m 49.1s on 2016-11-20.11222 UT. The OT unfiltered magnitude is (mlim=18.2m).

  14. MASTER-Tunka: bright 6mag ampl dwarf nova outburst and OT from MASTER-SAAO

    NASA Astrophysics Data System (ADS)

    Gress, O.; Balanutsa, P.; Lipunov, V.; Buckley, D.; Potter, S.; Budnev, N.; Ivanov, K.; Tiurina, N.; Gorbovskoy, E.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Chazov, V.; Kuvshinov, D.

    2016-11-01

    MASTER-Tunka auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 07h 37m 04.36s +25d 00m 54.5s on 2016-11-09.74453 UT. The OT unfiltered magnitude is (mlim=18.4m).

  15. MASTER-IAC: bright OT with ampltude more then 6.3mag

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Vladimirov, V.; Balanutsa, P.; Kuvshinov, D.; Kornilov, V.; Chazov, V.

    2016-11-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 12h 55m 32.84s -05d 04m 42.8s on 2016-11-17.26843 UT. The OT unfiltered magnitude is 15.7m (limit 17.5m).

  16. The 1000-th MASTER detection and SALT limit: Fast Doublet OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Kniazev, A.; Tiurina, N.; Kornilov, V.; Samus, N.; Kuznetsov, A.; Vlasenko, D.; Gorbunov, I.; Popova, E.; Vladimirov, V.; Shumkov, V.; Potter, S.; Kotze, M.; Gress, O.; Budnev, N.; Yazev, S.; Ivanov, K.; Tlatov, A.; Senik, V.; Dormidontov, D.; Parhomenko, A. V.; Yurkov, V.; Sergienko, Yu.; Gabovich, A.; Sinyakov, E.; Rebolo, R.; Serra-Ricart, M.; Lodieu, N.; Israelian, G.; Suarez-Andres, L.; Levato, Hugo; Astronomicas, Carlos Saffe Instituto de Ciencias; Espacio, de la Tierra y. del; Podesta, Ricardo; Mallamaci, Claudio; Lopez, Carlos; Podesta, Federico

    2015-12-01

    MASTER-SAAO (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171) discovered the 1000th OT source at (RA, Dec) = 05h 10m 14.58s -29d 09m 00.6s on 2015-12-16.97115 UT. The OT unfiltered magnitude is 18.8m (the limit is 20.4m).

  17. 12 CFR 563b.295 - Will OTS require me to re-solicit proxies?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Will OTS require me to re-solicit proxies? 563b.295 Section 563b.295 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY... to re-solicit proxies? If you amend your application for conversion, OTS may require you to...

  18. MASTER: high amplitude (>6.8m) optical transient and new OT

    NASA Astrophysics Data System (ADS)

    Gress, O.; Pogrosheva, T.; Lipunov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vladimirov, V.; Shumkov, V.; Kuvshinov, D.; Ivanov, K.; Budnev, N.; Tlatov, A.; Senik, V.

    2017-02-01

    MASTER-Tunka auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 19h 12m 48.12s +37d 49m 34.6s on 2017-01-31.86338 UT. The OT unfiltered magnitude is (limit 18.4m).

  19. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS compute time periods under this part? 516.10 Section 516.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In...

  20. Formation of octadecyltrichlorosilane (OTS) self-assembled monolayers on amorphous alumina

    NASA Astrophysics Data System (ADS)

    Kelkar, Sanket S.; Chiavetta, David; Wolden, Colin A.

    2013-10-01

    The kinetics and thermodynamics of octadecyltrichlorosilane (OTS) self-assembled monolayers (SAMs) on vapor deposited alumina were quantified. Ozone cleaning serves to create well-defined hydrophilic surfaces for OTS attachment, and the use of heptane as a solvent enables the formation of high quality SAMs under ambient conditions. The kinetics was characterized as a function of OTS concentration using contact angle goniometry, ellipsometry and Fourier transform infrared (FTIR) spectroscopy. The kinetics of SAM formation and the saturation contact angle (∼100̊) on alumina are comparable to what has been observed for OTS on silicon. The free energy of adsorption with ΔGads values ranged from -7.5 to -5.4 kcal/mol, and the SAMs were stable up to 230 ̊C. The critical surface tension of the OTS monolayer was found to be 21.4 dyne/cm.

  1. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  2. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  3. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  4. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  5. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you...

  6. 12 CFR 563b.200 - What actions may OTS take on my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... record under part 563e of this chapter and your business plan to determine how you will serve the... that you will serve. (2) OTS may deny your application if your business plan does not demonstrate...

  7. 12 CFR 563b.200 - What actions may OTS take on my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... record under part 563e of this chapter and your business plan to determine how you will serve the... that you will serve. (2) OTS may deny your application if your business plan does not demonstrate...

  8. Effects of Hypergravity Exposure on Plasma Oxytocin (OT) Concentrations in Pregnant and Lactating Rat Dams

    NASA Technical Reports Server (NTRS)

    Baer, Lisa A.; Wade, Charles E.; Plaut, Karen; Ronca, April E.; Dalton, Bonnie (Technical Monitor)

    2002-01-01

    From pregnancy to weaning there is a progressive elevation of plasma oxytocin (OT) levels associated with nursing activity, irrespective of litter size. In the present study, we analyzed the effects of continuous 1.5G, 1.75G and 2.0G hypergravity exposure on OT plasma concentration in prepartum (Gestation Day 20) (G20) and lactating (Postnatal day) (P10) rat dams. For this study, litter size was controlled with a yoking procedure established in our lab where individual control litters were yoked-matched to individual hypergravity litters. We reviewed all data at hypergravity irrespective of gravitational level and compared the values with the controls in both G20 (HG, n=15;SC, n=9) and P10 (HG, n=21;SC, n=16). Results showed that over time, we did observe the expected OT increase in both groups. In G20 dams, measurement of OT concentrations showed no significance. However, at P10, measurements of OT concentrations suggest a reduction of about 20% compared to established controls in our laboratory, 0.9+/-0.09 ng/ml for the controls and 0.7+/-0.06 ng/ml for centrifuged animals (p<0.02). These data suggest that exposure to centrifugation may reduce OT levels during lactation. When these plasma samples were obtained, the dams were removed from the litters, and values were not adjusted for the size of the litters. The reduction in OT with centrifugation may reflect a decrease in nursing activity or a decreased responsiveness of the mammary hypothalamic axis. In addition, we have analyzed data on plasma prolactin concentrations and mammary gland development, which may give additional insight to the results of our OT measurements.

  9. MASTER discovery: blue bright OT during FERMI 496473540 (GRB160925A) inspection

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Buckley, D.; Tiurina, N.; Gorbovskoy, E.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Vladimirov, V.; Shumkov, V.; Pogrosheva, T.; Rebolo Lopez, R.; Serra Ricart, M.; Podesta, R.; Levato, H.; Potter, S.; Lodieu, N.; Saffe, C.; Lopez, C.; Podesta, F.

    2016-09-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 22h 28m 17.90s -14d 56m 57.4s on 2016-09-25.75053 UT during automatically inspection of Fermi trigger 496473540 ( http://gcn.gsfc.nasa.gov/other/496473540.fermi ). The OT unfiltered magnitude is (limit 19.7m).

  10. OT2_smalhotr_3: Herschel Extreme Lensing Line Observations (HELLO)

    NASA Astrophysics Data System (ADS)

    Malhotra, S.

    2011-09-01

    We request 59.8 hours of Herschel time to observe 20 normal star-forming galaxies in the [CII] 158 micron and [OI] 63 micron lines. These galaxies lie at high redshift (1OT1, 49 high redshift IR luminous galaxies were approved for spectroscopy, but only two so-called normal galaxies were included. This is an imbalance that should be corrected, to balance Herschel's legacy.

  11. Oxytocin (OT) and arginine-vasopressin (AVP) act on OT receptors and not AVP V1a receptors to enhance social recognition in adult Syrian hamsters (Mesocricetus auratus).

    PubMed

    Song, Zhimin; Larkin, Tony E; Malley, Maureen O'; Albers, H Elliott

    2016-05-01

    Social recognition is a fundamental requirement for all forms of social relationships. A majority of studies investigating the neural mechanisms underlying social recognition in rodents have investigated relatively neutral social stimuli such as juveniles or ovariectomized females over short time intervals (e.g., 2h). The present study developed a new testing model to study social recognition among adult males using a potent social stimulus. Flank gland odors are used extensively in social communication in Syrian hamsters and convey important information such as dominance status. We found that the recognition of flank gland odors after a 3min exposure lasted for at least 24h, substantially longer than the recognition of other social cues in rats and mice. Intracerebroventricular injections of OT and AVP prolonged the recognition of flank gland odor for up to 48h. Selective OTR but not V1aR agonists, mimicked these enhancing effects of OT and AVP. Similarly, selective OTR but not V1aR antagonists blocked recognition of the odors after 20min. In contrast, the recognition of non-social stimuli was not blocked by either the OTR or the V1aR antagonists. Our findings suggest both OT and AVP enhance social recognition via acting on OTRs and not V1aRs and that the recognition enhancing effects of OT and AVP are limited to social stimuli.

  12. Short-Term H α Line Variations in Classical Be Stars: 59 Cyg and OT Gem

    NASA Astrophysics Data System (ADS)

    Paul, K. T.; Shruthi, S. B.; Subramaniam, Annapurni

    2017-03-01

    We present the optical spectroscopic study of two classical Be stars, 59 Cyg and OT Gem obtained over a period of few months in 2009. We detected a rare triple-peak H α emission phase in 59 Cyg and a rapid decrease in the emission strength of H α in OT Gem, which are used to understand their circumstellar disks. We find that 59 Cyg is likely to be rapid rotator, rotating at a fractional critical rotation of ˜0.80. The radius of the H α emission region for 59 Cyg is estimated to be R d/ R ∗ ˜ 10.0, assuming a Keplerian disk, suggesting that it has a large disk. We classify stars which have shown triple-peaks into two groups and find that the triple-peak emission in 59 Cyg is similar to ζ Tau. OT Gem is found to have a fractional critical rotation of ˜0.30, suggesting that it is either a slow rotator or viewed in low inclination. In OT Gem, we observed a large reduction in the radius of the H α emission region from ˜6.9 to ˜1.7 in a period of three months, along with the reduction in the emission strength. Our observations suggest that the disk is lost from outside to inside during this disk loss phase in OT Gem.

  13. Expression and developmental regulation of oxytocin (OT) and oxytocin receptors (OTR) in the enteric nervous system (ENS) and intestinal epithelium.

    PubMed

    Welch, Martha G; Tamir, Hadassah; Gross, Kara J; Chen, Jason; Anwar, Muhammad; Gershon, Michael D

    2009-01-10

    Although oxytocin (OT) and oxytocin receptor (OTR) are known for roles in parturition and milk let-down, they are not hypothalamus-restricted. OT is important in nurturing and opposition to stress. Transcripts encoding OT and OTR have been reported in adult human gut, and OT affects intestinal motility. We tested the hypotheses that OT is endogenous to the enteric nervous system (ENS) and that OTR signaling may participate in enteric neurophysiology. Reverse transcriptase polymerase chain reaction confirmed OT and OTR transcripts in adult mouse and rat gut and in precursors of enteric neurons immunoselected from fetal rats. Enteric OT and OTR expression continued through adulthood but was developmentally regulated, peaking at postnatal day 7. Coincidence of the immunoreactivities of OTR and the neural marker Hu was 100% in the P3 and 71% in the adult myenteric plexus, when submucosal neurons were also OTR-immunoreactive. Co-localization with NeuN established that intrinsic primary afferent neurons are OTR-expressing. Because OTR transcripts and protein were detected in the nodose ganglia, OT signaling might also affect extrinsic primary afferent neurons. Although OT immunoreactivity was found only in approximately 1% of myenteric neurons, extensive OT-immunoreactive varicosities surrounded many others. Villus enterocytes were OTR-immunoreactive through postnatal day 17; however, by postnatal day 19, immunoreactivity waned to become restricted to crypts and concentrated at crypt-villus junctions. Immunoelectron microscopy revealed plasmalemmal OTR at enterocyte adherens junctions. We suggest that OT and OTR signaling might be important in ENS development and function and might play roles in visceral sensory perception and neural modulation of epithelial biology.

  14. Expression and Developmental Regulation of Oxytocin (OT) and Oxytocin Receptors (OTR) in the Enteric Nervous System (ENS) and Intestinal Epithelium

    PubMed Central

    Welch, Martha G.; Tamir, Hadassah; Gross, Kara J.; Chen, Jason; Anwar, Muhammad; Gershon, Michael D.

    2011-01-01

    Although oxytocin (OT) and oxytocin receptor (OTR) are known for roles in parturition and milk let-down, they are not hypothalamus-restricted. OT is important in nurturing and opposition to stress. Transcripts encoding OT and OTR have been reported in adult human gut, and OT affects intestinal motility. We tested the hypotheses that OT is endogenous to the enteric nervous system (ENS) and that OTR signaling may participate in enteric neurophysiology. Reverse transcriptase polymerase chain reaction confirmed OT and OTR transcripts in adult mouse and rat gut and in precursors of enteric neurons immunoselected from fetal rats. Enteric OT and OTR expression continued through adulthood but was developmentally regulated, peaking at postnatal day 7. Coincidence of the immunoreactivities of OTR and the neural marker Hu was 100% in the P3 and 71% in the adult myenteric plexus, when submucosal neurons were also OTR-immunoreactive. Co-localization with NeuN established that intrinsic primary afferent neurons are OTR-expressing. Because OTR transcripts and protein were detected in the nodose ganglia, OT signaling might also affect extrinsic primary afferent neurons. Although OT immunoreactivity was found only in ~1% of myenteric neurons, extensive OT-immunoreactive varicosities surrounded many others. Villus enterocytes were OTR-immunoreactive through postnatal day 17; however, by postnatal day 19, immunoreactivity waned to become restricted to crypts and concentrated at crypt-villus junctions. Immunoelectron microscopy revealed plasmalemmal OTR at enterocyte adherens junctions. We suggest that OT and OTR signaling might be important in ENS development and function and might play roles in visceral sensory perception and neural modulation of epithelial biology. PMID:19003903

  15. 12 CFR 516.220 - If OTS requests additional information to complete my application, how will it process my...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... complete my application, how will it process my application? 516.220 Section 516.220 Banks and Banking... Standard Treatment § 516.220 If OTS requests additional information to complete my application, how will it... your response. OTS will notify you that it has extended the period before the end of the initial...

  16. 12 CFR 516.220 - If OTS requests additional information to complete my application, how will it process my...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... complete my application, how will it process my application? 516.220 Section 516.220 Banks and Banking... Standard Treatment § 516.220 If OTS requests additional information to complete my application, how will it... your response. OTS will notify you that it has extended the period before the end of the initial...

  17. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... condition. OTS determines the amount of each component under §§ 502.27 through 502.29 of this part. (b) For... reflect OTS costs of supervising the holding company structure and: (i) There are multiple top-tier holding companies in the holding company structure; (ii) The top-tier holding company is organized...

  18. 12 CFR 502.28 - How does OTS determine the organizational form component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... organizational form component by adding the base assessment to your risk/complexity component, and multiplying... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS determine the organizational form...-Calculation of Assessments § 502.28 How does OTS determine the organizational form component for a savings...

  19. Long non-coding RNA SOX2OT: expression signature, splicing patterns, and emerging roles in pluripotency and tumorigenesis

    PubMed Central

    Shahryari, Alireza; Jazi, Marie Saghaeian; Samaei, Nader M.; Mowla, Seyed J.

    2015-01-01

    SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA which harbors one of the major regulators of pluripotency, SOX2 gene, in its intronic region. SOX2OT gene is mapped to human chromosome 3q26.3 (Chr3q26.3) locus and is extended in a high conserved region of over 700 kb. Little is known about the exact role of SOX2OT; however, recent studies have demonstrated a positive role for it in transcription regulation of SOX2 gene. Similar to SOX2, SOX2OT is highly expressed in embryonic stem cells and down-regulated upon the induction of differentiation. SOX2OT is dynamically regulated during the embryogenesis of vertebrates, and delimited to the brain in adult mice and human. Recently, the disregulation of SOX2OT expression and its concomitant expression with SOX2 have become highlighted in some somatic cancers including esophageal squamous cell carcinoma, lung squamous cell carcinoma, and breast cancer. Interestingly, SOX2OT is differentially spliced into multiple mRNA-like transcripts in stem and cancer cells. In this review, we are describing the structural and functional features of SOX2OT, with an emphasis on its expression signature, its splicing patterns and its critical function in the regulation of SOX2 expression during development and tumorigenesis. PMID:26136768

  20. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... follows: (1) OTS will assess a base assessment amount of $3,500 on responsible savings and loan holding...-site supervision of a noncomplex, low risk savings and loan holding company structure. OTS will... savings and loan holding companies: a component based on the risk or complexity of the savings and...

  1. Spectroscopic Classification of MASTER OT J081506.13+381123.3 as a Type Ia Supernova

    NASA Astrophysics Data System (ADS)

    Rui, Liming; Li, Wenxiong; Chen, Zhihao; Xiang, Danfeng; Wang, Xiaofeng; Zhang, Tianmeng; Zhang, Jichen; Zhang, Ziyan; Luo, Zhe; Cui, Can; Li, Yaxin; ), Xinhang Shen

    2017-01-01

    We obtained an optical spectrum (range 360-860 nm) of MASTER OT J081506.13+381123.3 (see ATEL #9971), discovered by MASTER robotic Net, on UT Jan.20.6 2017 with the 2.16-m telescope (+BFOSC) at Xinglong Station of National Astronomical Observatories of China (NAOC).

  2. 12 CFR 516.15 - Must I meet with OTS before I file my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... with OTS before filing your application. You must submit a draft business plan before this meeting. (2... you to submit a draft business plan or other relevant information before this meeting. (b) Contacting... pre-filing meeting or a draft business plan, the Regional Office will determine whether it...

  3. 12 CFR 516.15 - Must I meet with OTS before I file my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... with OTS before filing your application. You must submit a draft business plan before this meeting. (2... you to submit a draft business plan or other relevant information before this meeting. (b) Contacting... pre-filing meeting or a draft business plan, the Regional Office will determine whether it...

  4. 12 CFR 563b.205 - May a court review OTS's final action on my conversion?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... Court of Appeals for the District of Columbia Circuit, to review the action under 12 U.S.C. 1464(i)(2)(B... 12 Banks and Banking 6 2013-01-01 2012-01-01 true May a court review OTS's final action on my conversion? 563b.205 Section 563b.205 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF...

  5. 12 CFR 563b.205 - May a court review OTS's final action on my conversion?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .... Court of Appeals for the District of Columbia Circuit, to review the action under 12 U.S.C. 1464(i)(2)(B... 12 Banks and Banking 6 2014-01-01 2012-01-01 true May a court review OTS's final action on my conversion? 563b.205 Section 563b.205 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF...

  6. 12 CFR 563b.205 - May a court review OTS's final action on my conversion?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .... Court of Appeals for the District of Columbia Circuit, to review the action under 12 U.S.C. 1464(i)(2)(B... 12 Banks and Banking 6 2012-01-01 2012-01-01 false May a court review OTS's final action on my conversion? 563b.205 Section 563b.205 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF...

  7. 12 CFR 563b.205 - May a court review OTS's final action on my conversion?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... Court of Appeals for the District of Columbia Circuit, to review the action under 12 U.S.C. 1464(i)(2)(B... 12 Banks and Banking 5 2011-01-01 2011-01-01 false May a court review OTS's final action on my conversion? 563b.205 Section 563b.205 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF...

  8. 12 CFR 563b.205 - May a court review OTS's final action on my conversion?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... Court of Appeals for the District of Columbia Circuit, to review the action under 12 U.S.C. 1464(i)(2)(B... 12 Banks and Banking 5 2010-01-01 2010-01-01 false May a court review OTS's final action on my conversion? 563b.205 Section 563b.205 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF...

  9. 78 FR 76768 - Removal of Transferred OTS Regulations Regarding Disclosure and Reporting of CRA-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ...In this notice of proposed rulemaking, the Federal Deposit Insurance Corporation (``FDIC'') proposes to rescind and remove a regulation entitled ``Disclosure and Reporting of CRA-Related Agreements.'' This regulation was included in the regulations that were transferred to the FDIC from the Office of Thrift Supervision (``OTS'') on July 21, 2011, in connection with the implementation of......

  10. Use of thermal cycling to reduce adhesion of OTS coated coated MEMS cantilevers

    NASA Astrophysics Data System (ADS)

    Ali, Shaikh M.; Phinney, Leslie M.

    2003-01-01

    °Microelectromechanical systems (MEMS) have enormous potential to contribute in diverse fields such as automotive, health care, aerospace, consumer products, and biotechnology, but successful commercial applications of MEMS are still small in number. Reliability of MEMS is a major impediment to the commercialization of laboratory prototypes. Due to the multitude of MEMS applications and the numerous processing and packaging steps, MEMS are exposed to a variety of environmental conditions, making the prediction of operational reliability difficult. In this paper, we investigate the effects of operating temperature on the in-use adhesive failure of electrostatically actuated MEMS microcantilevers coated with octadecyltrichlorosilane (OTS) films. The cantilevers are subjected to repeated temperature cycles and electrostatically actuated at temperatures between 25°C and 300°C in ambient air. The experimental results indicate that temperature cycling of the OTS coated cantilevers in air reduces the sticking probability of the microcantilevers. The sticking probability of OTS coated cantilevers was highest during heating, which decreased during cooling, and was lowest during reheating. Modifications to the OTS release method to increase its yield are also discussed.

  11. Optimal Diphthongs: An OT Analysis of the Acquisition of Spanish Diphthongs

    ERIC Educational Resources Information Center

    Krause, Alice

    2013-01-01

    This dissertation investigates the acquisition of Spanish diphthongs by adult native speakers of English. The following research questions will be addressed: 1) How do adult native speakers of English pronounce sequences of two vowels in their L2 Spanish at different levels of acquisition? 2) Can OT learnability models, specifically the GLA,…

  12. Pre-discovery observations of MASTER OT J004514.13+420007.2

    NASA Astrophysics Data System (ADS)

    Maehara, Hiroyuki

    2016-01-01

    I report pre-discovery observations of the possible nova in M31 MASTER OT J004514.13+420007.2 (ATel # 8603). The observations were performed with the 1.05-m Schmidt telescope and KWFC (Sako et al. 2012, SPIE, 8446, 84466L) at Kiso Observatory.

  13. 12 CFR 502.25 - How does OTS determine my complexity component?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ASSESSMENTS AND FEES Assessments Savings Associations-Calculation of Assessments § 502.25 How does OTS determine my complexity component? If you are a savings association and your portfolio exceeds any of the... paragraph (c) of this section. If your portfolio does not exceed any of the thresholds in paragraph (a)...

  14. 75 FR 76524 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    .... SUMMARY: The OTS Mutual Savings Associations Advisory Committee (MSAAC) will convene a meeting on Monday... meeting will be held on Monday, December 20, 2010, at Noon, Eastern Time. ADDRESSES: The meeting will be... Savings Association Advisory Committee will convene a closed meeting on Monday, December 20,...

  15. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS determine the risk/complexity...-Calculation of Assessments § 502.27 How does OTS determine the risk/complexity component for a savings and loan holding company? (a) OTS computes the risk/complexity component for responsible savings and...

  16. Effects of diethylenetriamine and Aerosol OT on the stability of oil-in-water emulsion stabilized by interfacial polyurea film.

    PubMed

    Mikami, T

    1994-01-01

    The stability of oil-in-water emulsions containing a triisocyanate soluble in the oil phase and an amine in the water phase was investigated. The oil component was di-n-butyl phthalate (DBP) containing Aerosol OT as an emulsifier. The time required for the average size parameter to reach a constant value was studied. It was found that the polyurea film produced by an interfacial polymerization reaction between the amine and the triisocyanate contributed to forming a stable emulsion at a lower Aerosol OT concentration, but at a higher Aerosol OT concentration the amine did not show any effect on the emulsion stability.

  17. MASTER-SAAO: bright PSN in PGC600519 and 2 OTs

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Gress, O.; Shumkov, V.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vladimirov, V.; Vlasenko, D.; Potter, S.

    2016-10-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 10h 54m 40.86s , -39d 13m 19s.0 on 2016-10-08 02:56:46.019UT with . This PSN is in 2.9"E,7"N from the center of PGC600519 and is seen on 8 images.

  18. MASTER: bright OT discovered during Fermi trigger 512353690/GRB170328A inspection

    NASA Astrophysics Data System (ADS)

    Gorbovskoy, E.; Lipunov, V.; Buckley, D.; Rebolo, R.; Serra-Ricart, M.; Gress, O.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Vladimirov, V.

    2017-03-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 45m 46.55s -35d 28m 47.6s on 2017-03-28.06645 UT during Fermi trigger 512353690(GRB170328A) inspection https://gcn.gsfc.nasa.gov/other/512353690.fermi (trigger time is 17/03/28 00:28:05.53UT).

  19. Computer software design description for the Treated Effluent Disposal Facility (TEDF), Project L-045H, Operator Training Station (OTS)

    SciTech Connect

    Carter, R.L. Jr.

    1994-11-07

    The Treated Effluent Disposal Facility (TEDF) Operator Training Station (OTS) is a computer-based training tool designed to aid plant operations and engineering staff in familiarizing themselves with the TEDF Central Control System (CCS).

  20. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... holding company structure) is a composite rating of 4 or 5, OTS will assess a charge under the condition... amount, the risk/complexity component, and any organizational form component. (b) For the purposes...

  1. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... holding company structure) is a composite rating of 4 or 5, OTS will assess a charge under the condition... amount, the risk/complexity component, and any organizational form component. (b) For the purposes...

  2. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... holding company structure) is a composite rating of 4 or 5, OTS will assess a charge under the condition... amount, the risk/complexity component, and any organizational form component. (b) For the purposes...

  3. Long Noncoding RNA-Sox2OT Knockdown Alleviates Diabetes Mellitus-Induced Retinal Ganglion Cell (RGC) injury.

    PubMed

    Li, Chao-Peng; Wang, Shu-Hong; Wang, Wen-Qi; Song, Shu-Guang; Liu, Xiu-Ming

    2017-03-01

    Retinal ganglion cell (RGC) injury is one of the important pathological features of diabetes-induced retinal neurodegeneration. Increasing attention has been paid to find strategies for protecting against RGC injury. Long noncoding RNAs (lncRNAs) have emerged as the key regulators of many cell functions. Here, we show that Sox2OT expression is significantly down-regulated in the retinas of STZ-induced diabetic mice and in the RGCs upon high glucose or oxidative stress. SOX2OT knockdown protects RGCs against high glucose-induced injury in vitro. Moreover, Sox2OT knockdown plays a neuroprotective role in diabetes-related retinal neurodegeneration in vivo. Sox2OT knockdown could regulate oxidative stress response in RGCs and diabetic mouse retinas. Sox2OT knockdown plays an anti-oxidative role via regulating NRF2/HO-1 signaling activity. Taken together, Sox2OT knockdown may be a therapeutic strategy for the prevention and treatment of diabetes-induced retinal neurodegeneration.

  4. Gene expression profiles of prohibitin in testes of Octopus tankahkeei (ot-phb) revealing its possible role during spermiogenesis.

    PubMed

    Mao, Hai-Tao; Wang, Da-Hui; Lan, Zhou; Zhou, Hong; Yang, Wan-Xi

    2012-05-01

    Prohibitin is essential for intracellular homeostasis and stabilization of mitochondrial respiratory chain complexes. To explore its functions during spermiogenesis of Octopus tankahkeei (O. tankahkeei), we have cloned and sequenced the cDNA of this mammalian PHB homologue (termed ot-PHB) from the testes of O. tankahkeei. The 1165 bp ot-phb cDNA contains a 100 bp 5' UTR, a 882 bp open reading frame and a 183 bp 3' UTR. The putative ot-PHB protein owns a transmembrane domain from 6 to 31 amino acid (aa) and a putative PHB domain from 26 to 178 aa. Protein alignment demonstrated that ot-PHB had 73.3, 73.6, 74.0, 75.1, and 45.4% identity with its homologues in Homo sapiens, Mus muculus, Danio rerio, Xenopus tropicalis and Trypanosoma brucei, respectively. Tissue distribution profile analysis revealed its presence in all the tissues examined. In situ hybridization in spermiogenic cells demonstrated that ot-phb was expressed moderately at the beginning of the spermiogenesis. The abundance of transcripts increased in intermediate spermatids and in drastically remodeling final spermatids. In mature spermatozoa, the residuary transcripts concentrated around the chondriosomal mantle where mitochondria assemble around. In summary, the expression of ot-phb during spermiogenesis implicates a potential function of this protein during mitochondrial ubiquitination. It is the first time to implicate the role of prohibitin in cephalopod spermiogenesis.

  5. Structural insight into dephosphorylation by trehalose 6-phosphate phosphatase (OtsB2) from Mycobacterium tuberculosis.

    PubMed

    Shan, Shan; Min, Haowei; Liu, Ting; Jiang, Dunquan; Rao, Zihe

    2016-12-01

    Trehalose serves as a key structural component in the cell wall of Mycobacterium tuberculosis. M. tuberculosis trehalose-6-phosphate phosphatase (MtbTPP), an essential enzyme in the trehalose biosynthesis OtsAB pathway, catalyzes the dephosphorylation of trehalose-6-phosphate (trehalose-6-P) to generate trehalose, and plays a critical role in M. tuberculosis survival-associated cell wall formation and permeability. Therefore, MtbTPP (OtsB2) is considered a promising potential target for discovery of antimicrobial drugs. However, the absence of structural information of MtbTPP restrains our understanding of its underlying catalytic mechanism. Here, we report the high-resolution crystal structures of apo active MtbTPP and its trehalose-6-P bound complex. The apo structure presents a canonical haloacid dehalogenase superfamily structural fold plus an extra N-terminal domain. The catalytic center is located in a positively charged cleft between the hydrolase domain and the cap domain, demonstrating a highly conserved substrate binding pocket. The role of residues interacting with the substrate in catalysis were probed by site-directed mutagenesis. Asp147, Asp149, Asp330, and Asp331 were found to be pivotal for the enzymatic activity of MtbTPP. The MtbTPP structures reported here provide insight into a key step in the biosynthesis of trehalose, which would facilitate future development of anti-TB therapeutics.-Shan, S., Min, H., Liu, T., Jiang, D., Rao, Z. Structural insight into dephosphorylation by trehalose 6-phosphate phosphatase (OtsB2) from Mycobacterium tuberculosis.

  6. Spectroscopic classification of PSN J13144705+5405055 (= MASTER OT J131447.05+540505.5)

    NASA Astrophysics Data System (ADS)

    Vanni, S.; Pastorello, A.; Benetti, S.; Cappellaro, E.; Ochner, P.; Tomasella, L.; Turatto, M.

    2012-11-01

    We report that optical spectroscopy (range 340-820 nm; resolution 1.3 nm) obtained on Nov. 8.18 UT with the Asiago 1.82-m Copernico Telescope (+ AFOSC), shows that PSN J13144705+5405055 (= MASTER OT J131447.05+540505.5) is a type-Ia SN at a redshift z~0.033 (Sloan Digital Sky Survey Data Release 3). The best match was found with type-Ia SN 2002bo (Benetti et al. 2004, MNRAS, 348, 261) about 3-4 weeks after the B band maximum light.

  7. MASTER OT J095503.19-355149.4 - a probable Dwarf Nova

    NASA Astrophysics Data System (ADS)

    Buckley, D. A. H.; Kniazev, A.; Potter, S. B.; Lipunov, V.; Tiurina, N.; Gorbovskoy, E.

    2016-02-01

    Followup optical imaging and spectroscopic observations with SALT of the optical transient MASTER OT J095503.19-355149.4, tentatively identified (ATel #8741) as the optical counterpart of the Fermi GBM trigger 478121068 (GCN #19092), were conducted on 2016-02-26.78 UT. From un-calibrated g', r' and i' exposures (30 sec each) with the SALTICAM CCD camera, the transient appeared to be approximately 1 mag brighter (V ~ 17) than the MASTER-SAAO observations of the previous night (reported in ATel #8741) and was also seen to be a relatively red object compared to other stars in the field.

  8. Complete Genome Sequence of the Larvicidal Bacterium Lysinibacillus sphaericus Strain OT4b.25

    PubMed Central

    Rey, Andrés; Silva-Quintero, Laura

    2016-01-01

    Lysinibacillus sphaericus OT4b.25 is a native Colombian strain isolated from coleopteran larvae in an oak forest near Bogotá D.C.; this strain has shown high levels of pathogenic activity against Culex quinquefasciatus larvae in laboratory assays compared to that of other members of the same species. Using Pacific Biosciences sequencing technology, we propose a chromosomal contig of 4,665,775 bp that, according to comparative analysis, is highly similar to that of reference strain L. sphaericus C3-41. PMID:27151786

  9. Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

    NASA Technical Reports Server (NTRS)

    Mathur, A. K.; Pederson, S.

    1982-01-01

    The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

  10. DeSSpOt: an instrument for stellar spin orientation determination

    NASA Astrophysics Data System (ADS)

    Lesage, Anna-Lea; Schneide, Magnus; Wiedemann, Günter

    2012-09-01

    We designed and constructed a special instrument to enable the determination of the stellar's spin orientation. The Differential image rotator for Stellar Spin Orientation, DeSSpOt, allows the simultaneous observations of two anti-parallel orientations of the star on the spectrum. On a high resolution échelle spectrum, the stellar rotation causes a slight line tilt visible in the spatial direction which is comparable to a rotation curve. We developed a new method, which exploits the variations in these tilts, to estimate the absolute position angle of the rotation axis. The line tilt is retrieved by a spectroastrometric extraction of the spectrum. In order to validate the method, we observed spectroscopic binaries with known orbital parameters. The determination of the orbital position angle is equivalent to the determination of the stellar position angle, but is easier to to detect. DeSSpOt was successfully implemented on the high resolution Coudé spectrograph of the Thüringer Landessternwarte Tautenburg. The observations of Capella led to the determination of the orbital position angle. Our value of 37.2° is in agreement with the values previously found in the literature. As such we verified that both method and instrument are valid.

  11. Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

    NASA Astrophysics Data System (ADS)

    Mathur, A. K.; Pederson, S.

    1982-08-01

    The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

  12. Solar energy system performance evaluation - final report for Honeywell OTS 45, Salt River Project, Phoenix, Arizona

    SciTech Connect

    Mathur, A K

    1983-09-01

    This report describes the operation and technical performance of the Solar Operational Test Site (OTS 45) at Salt River Project in Phoenix, Arizona, based on the analysis of data collected between April 1981 and March 31, 1982. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 45 is a hydronic heating and cooling system consisting of 8208 square feet of liquid-cooled flat-plate collectors; a 2500-gallon thermal storage tank; two 25-ton capacity organic Rankine-cycle-engine-assisted water chillers; a forced-draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics. Based on the instrumented test data monitored and collected during the 8 months of the Operational Test Period, the solar system collected 1143 MMBtu of thermal energy of the total incident solar energy of 3440 MMBtu and provided 241 MMBtu for cooling and 64 MMBtu for heating. The projected net annual electrical energy savings due to the solar system was approximately 40,000 kWh(e).

  13. Solar energy system performance evaluation: Honeywell OTS 41, Shenandoah (Newman), Georgia

    NASA Astrophysics Data System (ADS)

    Mathur, A. K.; Pederson, S.

    1982-08-01

    The operation and technical performance of the Solar Operational Test Site (OTS 41) located at Shenandoah, Georgia, are described, based on the analysis of the data collected between January and August 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 41 is a hydronic heating and cooling system consisting of 702 square feet of liquid-cooled flat-plate collectors; a 1000-gallon thermal storage tank; a 3-ton capacity organic Rankine-cycle-engine-assisted air conditioner; a water-to-are heat exchanger for solar space heating; a finned-tube coil immersed in the storage tank to preheat water for a gas-fired hot water heater; and associated piping, pumps, valves, and controls. The solar system has six basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics.

  14. Genome sequence and description of the heavy metal tolerant bacterium Lysinibacillus sphaericus strain OT4b.31

    PubMed Central

    Peña-Montenegro, Tito David; Dussán, Jenny

    2013-01-01

    Lysinibacillus sphaericus strain OT4b.31 is a native Colombian strain having no larvicidal activity against Culex quinquefasciatus and is widely applied in the bioremediation of heavy-metal polluted environments. Strain OT4b.31 was placed between DNA homology groups III and IV. By gap-filling and alignment steps, we propose a 4,096,672 bp chromosomal scaffold. The whole genome (consisting of 4,856,302 bp long, 94 contigs and 4,846 predicted protein-coding sequences) revealed differences in comparison to the L. sphaericus C3-41 genome, such as syntenial relationships, prophages and putative mosquitocidal toxins. Sphaericolysin B354, the coleopteran toxin Sip1A and heavy metal resistance clusters from nik, ars, czc, cop, chr, czr and cad operons were identified. Lysinibacillus sphaericus OT4b.31 has applications not only in bioremediation efforts, but also in the biological control of agricultural pests. PMID:24501644

  15. Kcnq1ot1/Lit1 Noncoding RNA Mediates Transcriptional Silencing by Targeting to the Perinucleolar Region ▿ †

    PubMed Central

    Mohammad, Faizaan; Pandey, Radha Raman; Nagano, Takashi; Chakalova, Lyubomira; Mondal, Tanmoy; Fraser, Peter; Kanduri, Chandrasekhar

    2008-01-01

    The Kcnq1ot1 antisense noncoding RNA has been implicated in long-range bidirectional silencing, but the underlying mechanisms remain enigmatic. Here we characterize a domain at the 5′ end of the Kcnq1ot1 RNA that carries out transcriptional silencing of linked genes using an episomal vector system. The bidirectional silencing property of Kcnq1ot1 maps to a highly conserved repeat motif within the silencing domain, which directs transcriptional silencing by interaction with chromatin, resulting in histone H3 lysine 9 trimethylation. Intriguingly, the silencing domain is also required to target the episomal vector to the perinucleolar compartment during mid-S phase. Collectively, our data unfold a novel mechanism by which an antisense RNA mediates transcriptional gene silencing of chromosomal domains by targeting them to distinct nuclear compartments known to be rich in heterochromatic machinery. PMID:18299392

  16. Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice

    PubMed Central

    Koliwer-Brandl, Hendrik; Hartman, Travis; Jacobs, William R.; Ioerger, Thomas R.; Ehrt, Sabine

    2016-01-01

    Trehalose biosynthesis is considered an attractive target for the development of antimicrobials against fungal, helminthic and bacterial pathogens including Mycobacterium tuberculosis. The most common biosynthetic route involves trehalose-6-phosphate (T6P) synthase OtsA and T6P phosphatase OtsB that generate trehalose from ADP/UDP-glucose and glucose-6-phosphate. In order to assess the drug target potential of T6P phosphatase, we generated a conditional mutant of M. tuberculosis allowing the regulated gene silencing of the T6P phosphatase gene otsB2. We found that otsB2 is essential for growth of M. tuberculosis in vitro as well as for the acute infection phase in mice following aerosol infection. By contrast, otsB2 is not essential for the chronic infection phase in mice, highlighting the substantial remodelling of trehalose metabolism during infection by M. tuberculosis. Blocking OtsB2 resulted in the accumulation of its substrate T6P, which appears to be toxic, leading to the self-poisoning of cells. Accordingly, blocking T6P production in a ΔotsA mutant abrogated otsB2 essentiality. T6P accumulation elicited a global upregulation of more than 800 genes, which might result from an increase in RNA stability implied by the enhanced neutralization of toxins exhibiting ribonuclease activity. Surprisingly, overlap with the stress response caused by the accumulation of another toxic sugar phosphate molecule, maltose-1-phosphate, was minimal. A genome-wide screen for synthetic lethal interactions with otsA identified numerous genes, revealing additional potential drug targets synergistic with OtsB2 suitable for combination therapies that would minimize the emergence of resistance to OtsB2 inhibitors. PMID:27936238

  17. Entraining IDyOT: Timing in the Information Dynamics of Thinking.

    PubMed

    Forth, Jamie; Agres, Kat; Purver, Matthew; Wiggins, Geraint A

    2016-01-01

    We present a novel hypothetical account of entrainment in music and language, in context of the Information Dynamics of Thinking model, IDyOT. The extended model affords an alternative view of entrainment, and its companion term, pulse, from earlier accounts. The model is based on hierarchical, statistical prediction, modeling expectations of both what an event will be and when it will happen. As such, it constitutes a kind of predictive coding, with a particular novel hypothetical implementation. Here, we focus on the model's mechanism for predicting when a perceptual event will happen, given an existing sequence of past events, which may be musical or linguistic. We propose a range of tests to validate or falsify the model, at various different levels of abstraction, and argue that computational modeling in general, and this model in particular, can offer a means of providing limited but useful evidence for evolutionary hypotheses.

  18. Entraining IDyOT: Timing in the Information Dynamics of Thinking

    PubMed Central

    Forth, Jamie; Agres, Kat; Purver, Matthew; Wiggins, Geraint A.

    2016-01-01

    We present a novel hypothetical account of entrainment in music and language, in context of the Information Dynamics of Thinking model, IDyOT. The extended model affords an alternative view of entrainment, and its companion term, pulse, from earlier accounts. The model is based on hierarchical, statistical prediction, modeling expectations of both what an event will be and when it will happen. As such, it constitutes a kind of predictive coding, with a particular novel hypothetical implementation. Here, we focus on the model's mechanism for predicting when a perceptual event will happen, given an existing sequence of past events, which may be musical or linguistic. We propose a range of tests to validate or falsify the model, at various different levels of abstraction, and argue that computational modeling in general, and this model in particular, can offer a means of providing limited but useful evidence for evolutionary hypotheses. PMID:27803682

  19. How Surface Heterogeneity Affects Protein Adsorption: Annealing of OTS Patterns and Albumin Adsorption Kinetics*

    PubMed Central

    Hodgkinson, Gerald N.; Hlady, Vladimir

    2009-01-01

    Fluorescence microscopy and intensity histogram analysis techniques were used to monitor spatially-resolved albumin adsorption kinetics to model heterogeneous surfaces on sub-μm scales. Several distinct protein subpopulations were resolved, each represented by a normal distribution of adsorption densities on the adsorbent surface. Histogram analyses provided dynamic information of mean adsorption density, spread in adsorption density, and surface area coverage for each distinct protein subpopulation. A simple adsorption model is proposed in which individual protein binding events are predicted by the summation of multiple protein's surface sub-site interactions with different binding energy sub-sites on adsorbent surfaces. This model is predictive of the albumin adsorption on the patterns produced by one step μ-contact printing (μCP) of octadecyltrichlorosilane (OTS) on glass but fails to describe adsorption once the same patterns are altered by a thermal annealing step. PMID:19746205

  20. The Persistent Eruption of UGC 2773-OT: finally, a decade-long extragalactic Eta Carinae analogue

    NASA Astrophysics Data System (ADS)

    Smith, Nathan; Andrews, Jennifer E.; Mauerhan, Jon C.; Zheng, WeiKang; Filippenko, Alexei V.; Graham, Melissa L.; Milne, Peter

    2016-02-01

    While supernova (SN) impostors resemble the Great Eruption of η Carinae in the sense that their spectra show narrow H lines and they have typical peak absolute magnitudes of -13 to -14 mag, most extragalactic events observed so far are quite different from η Car in duration. Their bright phases typically last for ˜100 d or less, rather than persisting for several years. The transient object UGC 2773-OT (discovered in 2009) had a similar peak absolute magnitude to other SN impostors, but with a gradual 5-yr pre-discovery rise. In the ˜6 yr since discovery, it has faded very slowly (0.26 mag yr-1). Overall, we suggest that its decade-long eruption is so far the best-known analogue of η Car's 19th century eruption. We discuss extensive spectroscopy of the ongoing eruption. The spectra show interesting changes in velocity and line shape that we discuss in detail, including an asymmetric Hα emission line that we show is consistent with the ejection of a bipolar nebula that could be very much like the Homunculus of η Car. Moreover, changes in the line width, line profile, blue excess emission resembling that of Type IIn SNe, and the intensity of Hα suggest the presence of strong circumstellar interaction in the eruption at late times. This supports the hypothesis that the extended plateau of η Car's eruption may have been powered by shock interaction as well. One interesting difference compared to η Car, however, is that UGC 2773-OT so far does not exhibit the repeated brief spikes in luminosity that have been associated with binary periastron events.

  1. Solar energy system performance evaluation: final report for Honeywell OTS 41, Shenandoah (Newnan), Georgia

    SciTech Connect

    Mathur, A K; Pederson, S

    1982-08-01

    The operation and technical performance of the Solar Operational Test Site (OTS 41) located at Shenandoah, Georgia, are described, based on the analysis of data collected between January and August 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 41 is a hydronic heating and cooling system consisting of 702 square feet of liquid-cooled flat-plate collectors; a 1000-gallon thermal storage tank; a 3-ton capacity organic Rankine-cycle-engine-assisted air conditioner; a water-to-air heat exchanger for solar space heating; a finned-tube coil immersed in the storage tank to preheat water for a gas-fired hot water heater; and associated piping, pumps, valves, and controls. The solar system has six basic modes of operation and several combination modes. The system operation is controlled automatically by a Honeywell-designed microprocessor-based control system, which also provides diagnostics. Based on the instrumented test data monitored and collected during the 7 months of the Operational Test Period, the solar system collected 53 MMBtu of thermal energy of the total incident solar energy of 219 MMBtu and provided 11.4 MMBtu for cooling, 8.6 MMBtu for heating, and 8.1 MMBtu for domestic hot water. The projected net annual energy savings due to the solar system were approximately 50 MMBtu of fossil energy (49,300 cubic feet of natural gas) and a loss of 280 kWh(e) of electrical energy.

  2. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and... within two calendar years after the filing date? (a) Withdrawal. If OTS has not approved or denied your pending application within two calendar years after the filing date under § 516.45, OTS will notify...

  3. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and... within two calendar years after the filing date? (a) Withdrawal. If OTS has not approved or denied your pending application within two calendar years after the filing date under § 516.45, OTS will notify...

  4. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and... within two calendar years after the filing date? (a) Withdrawal. If OTS has not approved or denied your pending application within two calendar years after the filing date under § 516.45, OTS will notify...

  5. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and... within two calendar years after the filing date? (a) Withdrawal. If OTS has not approved or denied your pending application within two calendar years after the filing date under § 516.45, OTS will notify...

  6. 12 CFR 516.290 - What will happen if OTS does not approve or disapprove my application within two calendar years...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... disapprove my application within two calendar years after the filing date? 516.290 Section 516.290 Banks and... within two calendar years after the filing date? (a) Withdrawal. If OTS has not approved or denied your pending application within two calendar years after the filing date under § 516.45, OTS will notify...

  7. Spectroscopic Classification of AT2016axw (=MASTER OT J142718.40-014031.0 =PS16axi)

    NASA Astrophysics Data System (ADS)

    Blanchard, Peter; Berger, Edo; Bieryla, Allyson; Brzycki, Bryan; Cohn, Amy; Dillett, Sean; Feder-Staehle, Richard; Frostig, Danielle; Grell, Gabe; James, Nicholas; Johnson, April; Mahmood, Zahra; Merryman, Hunter; Peak, Barra; Sanders, Cecilia; Taylor, Anthony

    2016-03-01

    We obtained a low-resolution optical spectrum of AT2016axw (=MASTER OT J142718.40-014031.0 =PS16ayd; ATel #8791). Observations were carried out on 2016 March 16.4 UT using the FAST spectrograph mounted on the F.L. Whipple Observatory 1.5-m telescope.

  8. Genome sequence of Desulfosporosinus sp. OT, an acidophilic sulfate-reducing bacterium from copper mining waste in Norilsk, Northern Siberia.

    PubMed

    Abicht, Helge K; Mancini, Stefano; Karnachuk, Olga V; Solioz, Marc

    2011-11-01

    We have sequenced the genome of Desulfosporosinus sp. OT, a Gram-positive, acidophilic sulfate-reducing Firmicute isolated from copper tailing sediment in the Norilsk mining-smelting area in Northern Siberia, Russia. This represents the first sequenced genome of a Desulfosporosinus species. The genome has a size of 5.7 Mb and encodes 6,222 putative proteins.

  9. Correction to ATel 10150: New OT is PTSS-17jdx deteted on 2017-03-07.727524 UT

    NASA Astrophysics Data System (ADS)

    Li, Bin

    2017-03-01

    We report a correction to the time of detection of new OT PTSS-17jdx in ATel #10150. The time of detection is 2017-03-07.727524 UT but not 2017-02-07.727524 UT. More interesting information can be found on PTSS . We apologize for any confusion caused by this typo error.

  10. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-site supervision of a noncomplex, low risk savings and loan holding company structure. OTS will... savings and loan holding companies: a component based on the risk or complexity of the savings and loan holding company's business, a component based on its organizational form, and a component based on...

  11. 75 FR 63107 - Alternatives to the Use of External Credit Ratings in the Regulations of the OTS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ..., savings associations must consider the interest rate, credit, liquidity, price and other risks presented... subject only to moderate credit risk, and for whom expectations of default risk over the term of the... the levels and types of risks in its portfolio. As with the credit quality standard, OTS could...

  12. Purification, crystallization and preliminary crystallographic analysis of the biotin–protein ligase from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Kuroishi, Chizu; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-02-01

    The biotin–protein ligase from P. horikoshii OT3 was overexpressed, purified, crystallized and cocrystallized with biotin, ADP and biotinyl-5′-AMP. The crystals belong to space group P2{sub 1} and diffract to beyond 1.6 Å resolution.

  13. Theileria sp. OT3 and other tick-borne pathogens in sheep and ticks in Italy: molecular characterization and phylogeny.

    PubMed

    Giangaspero, A; Marangi, M; Papini, R; Paoletti, B; Wijnveld, M; Jongejan, F

    2015-02-01

    PCR Reverse Line Blot (RLB) hybridization and sequencing were used to determine the dynamics of infection with tick-borne pathogens in one hundred apparently healthy sheep in Italy. Blood samples were tested once prior to the onset of the grazing season (June 2010) and once after the end of the grazing season (August 2010). Ticks collected from sheep and from the vegetation were also tested by PCR/RLB. Before grazing, 56% of the sheep harbored several tick-borne pathogens: Anaplasma ovis was the most prevalent (41%), followed by A. ovis co-infected with Theileria sp. OT3 (14%). After grazing, 87% of sheep were positive for A. ovis alone (41%), co-infected with Theileria sp. OT3 (8%) or co-infected with Babesia motasi (5%). Other sheep were infected with Anaplasma phagocytophilum alone (20%), co-infected with B. motasi (7%) or with Theileria sp. OT3 (5%) (p<0.001). After grazing, sheep were significantly more infected with tick-borne pathogens than before grazing. Ticks collected were all Haemaphysalis punctata (n-89) and 36% were positive for A. ovis, Ehrlichia ovina and A. ovis combined with A. phagocytophilum. Phylogenetic analysis including isolates from countries in the Mediterranean Basin show circulation of the same variants of Theileria sp. OT3, whereas two different geographical origins for the isolates of A. ovis and A. phagocytophilum were identified. This is the first report from Italy of Theileria sp. OT3 in sheep, whereas the detection of Ehrlichia ovina in ticks is worth noting, and the presence of A. phagocytophilum in sheep and in ticks poses a potential public health risk.

  14. A new microRNA signal pathway regulated by long noncoding RNA TGFB2-OT1 in autophagy and inflammation of vascular endothelial cells

    PubMed Central

    Huang, ShuYa; Lu, Wei; Ge, Di; Meng, Ning; Li, Ying; Su, Le; Zhang, ShangLi; Zhang, Yun; Zhao, BaoXiang; Miao, JunYing

    2015-01-01

    TGFB2-OT1 (TGFB2 overlapping transcript 1) is a newly discovered long noncoding RNA (lncRNA) derived from the 3′UTR of TGFB2. It can regulate autophagy in vascular endothelial cells (VECs). However, the mechanisms of TGFB2-OT1 action are unclear, and whether it is involved in VECs dysfunction needs investigation. Here, the level of TGFB2-OT1 was markedly increased by lipopolysaccharide and oxidized low-density lipoprotein, 2 VECs inflammation triggers. A chemical small molecule, 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO) significantly decreased TGFB2-OT1 levels and inhibited the effect of LPS and oxLDL. The NUPR1 level was upregulated by the 2 inflammation inducers and modulated the TGFB2-OT1 level by promoting the expression of TIA1, responsible for TGFB2-OT1 processing. We focused on how TGFB2-OT1 regulated autophagy and inflammation. Use of miRNA chip assay, TGFB2-OT1 overexpression technology and 3BDO revealed that TGFB2-OT1 regulated the levels of 3 microRNAs, MIR3960, MIR4488 and MIR4459. Further studies confirmed that TGFB2-OT1 acted as a competing endogenous RNA, bound to MIR3960, MIR4488 and MIR4459, then regulated the expression of the miRNA targets CERS1 (ceramide synthase 1), NAT8L (N-acetyltransferase 8-like [GCN5-related, putative]), and LARP1 (La ribonucleoprotein domain family, member 1). CERS1 and NAT8L participate in autophagy by affecting mitochondrial function. TGFB2-OT1 increased the LARP1 level, which promoted SQSTM1 (sequestosome 1) expression, NFKB RELA and CASP1 activation, and then production of IL6, IL8 and IL1B in VECs. Thus, NUPR1 and TIA1 may control the level of TGFB2-OT1, and TGFB2-OT1 bound to MIR3960, MIR4488 and MIR4459, which targeted CERS1, NAT8L, and LARP1, respectively, the key proteins involved in autophagy and inflammation. PMID:26565952

  15. Effect of Ozone Therapy (OT) on Healing of Colonic Anastomosis in a Rat Model of Peritonitis

    PubMed Central

    Erginel, Başak; Erginel, Turgay; Aksoy, Bilgin; Dokucu, Ali İhsan

    2014-01-01

    Background: Ozone is a three-oxygen molecule (O3). Ozone therapy (OT) is systematically effective when pathological inflammatory and immunologic processes are activated. Among of these conditions are wound healing, macular degeneration related to aging, and conditions that are ischemic or infectious. Aims: The aim of this study was to determine the effects of OT on wound healing of intestinal anastomosis in the presence of peritonitis in a rat model. Study Design: Animal experimentation. Methods: A total of 40 Wistar albino rats were randomized into four groups (n=10) including: sham (S), peritonitis (P), ozone 0 (O0), and ozone 24 (O24). In group S, only cecal dissection was carried out. The S group had only a cecal dissection and intestinal anastomosis performed, but no peritonitis. In all other groups, cecal ligation and puncture (CLP) followed the cecal dissection to induce bacterial peritonitis. 24 h after puncture, a cecal resection and ileocolic anastomosis were performed. In group P, 24 h after CLP, a cecal resection and ileocolic anastomosis were performed and no ozone was administered. In group O0, immediately after the anastomosis, and in group O24, starting 24 hours after the anastomosis, an intraperitoneal 1 mg/kg/day ozone administration was applied for seven days. On the seventh day the animals were sacrificed, the anastomotic bursting pressures (BP) and the hydroxyproline values of the anastomotic tissues were measured, and histopathologic examination of the anastomotic segment was carried out. Results: The highest BP was in group S, with 211±23.13 mmHg. The mean BP of group P was 141±56.25 mmHg, which was significantly lower than in the other two peritonitis groups that received ozone therapy, group O0 and O24, where it was 192±22 and 166±45 mmHg, respectively (p<0.05). The difference in the BP between groups O0 and O24 was not statistically significant (p>0.05). Histopathologic analyses of the anastomotic segments determined there was

  16. An AFM Study of the Effects of Silanization Temperature, Hydration, and Annealing on the Nucleation and Aggregation of Condensed OTS Domains on Mica.

    PubMed

    Britt, David W; Hlady, Vladimir

    1996-03-25

    Partial monolayers of octadecyltrichlorosilane (OTS) were formed on mica under different reaction conditions in which the silanization temperature, time, and amount of water adsorbed on the mica substrates were varied. OTS surface coverage increased with silanization time for all samples; however, the amount and distribution of adsorbed OTS varied greatly under these different reaction conditions. AFM analysis showed that OTS formed two phases on mica silanized at 25°C: condensed "island-like" domains and expanded "liquid-like" domains. Partially dehydrated mica silanized at 9°C, however, displayed only condensed domains which were of smaller size compared to those on the 25°C samples. The lateral diffusion and aggregation of small condensed OTS domains to form larger aggregates was evident on all surfaces except the 25°C partially dehydrated mica. A uniform distribution of many small condensed domains surrounded by expanded OTS phases was seen instead. Extended annealing resulted in surface diffusion and aggregation of these domains and nucleation of new condensed domains from the surrounding expanded OTS phases. These observations are consistent with a deposition, diffusion, and aggregation model (DDA) which allows for activated diffusion; however, rigorous modeling is not presented here.

  17. Over-Expression of the Mycobacterial Trehalose-Phosphate Phosphatase OtsB2 Results in a Defect in Macrophage Phagocytosis Associated with Increased Mycobacterial-Macrophage Adhesion

    PubMed Central

    Li, Hao; Wu, Mei; Shi, Yan; Javid, Babak

    2016-01-01

    Trehalose-6-phosphate phosphatase (OtsB2) is involved in the OtsAB trehalose synthesis pathway to produce free trehalose and is strictly essential for mycobacterial growth. We wished to determine the effects of OtsB2 expression on mycobacterial phenotypes such as growth, phagocytosis and survival in macrophages. Mycobacterium bovis-bacillus calmette-guerin (BCG) over-expressing OtsB2 were able to better survive in stationary phase. Over-expression of OtsB2 led to a decrease in phagocytosis but not survival in THP-1 macrophage-like cells, and this was not due to a decrease in general macrophage phagocytic activity. Surprisingly, when we investigated macrophage–mycobacterial interactions by flow cytometry and atomic force microscopy, we discovered that BCG over-expressing OtsB2 have stronger binding to THP-1 cells than wild-type BCG. These results suggest that altering OtsB2 expression has implications for mycobacterial host–pathogen interactions. Macrophage–mycobacteria phagocytic interactions are complex and merit further study. PMID:27867377

  18. Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    PubMed Central

    Barber, Carmel M.; Ahmad Rusmili, Muhamad Rusdi; Hodgson, Wayne C.

    2016-01-01

    Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1–1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA2 value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins. PMID:26938558

  19. Enhancement of the skin permeation of clindamycin phosphate by Aerosol OT/1-butanol microemulsions.

    PubMed

    Junyaprasert, Varaporn Buraphacheep; Boonsaner, Panee; Leatwimonlak, Sujitra; Boonme, Prapaporn

    2007-08-01

    Microemulsions of water/isopropyl palmitate (IPP)/Aerosol OT (AOT)/1-butanol were developed as alternative formulations for topical delivery of clindamycin phosphate. Effect of AOT:1-butanol ratios on microemulsion region existence in the pseudoternary phase diagrams was investigated. The 2:1 AOT:1-butanol provided the largest microemulsion region. Five microemulsions of 1% w/w clindamycin phosphate were prepared and characterized. The permeation through human epidermis of the microemulsions was evaluated and compared with the 70% isopropanol solution using modified Franz diffusion cells. The drug permeation from all microemulsions was found to be significantly greater than that from the solution, indicating the enhancement of the skin permeation by the microemulsions. Within the same microemulsion type, the drug permeation increased with increasing the amount of AOT:1-butanol. The drug permeation from oil-in-water (o/w) microemulsions was relatively higher than that from water-in-oil (w/o) microemulsions. In addition, all microemulsions were stable for at least three months at 30 +/- 1 degrees C.

  20. Almost gone: SN 2008S and NGC 300 2008OT-1 are fainter than their progenitors

    NASA Astrophysics Data System (ADS)

    Adams, S. M.; Kochanek, C. S.; Prieto, J. L.; Dai, X.; Shappee, B. J.; Stanek, K. Z.

    2016-08-01

    We present late-time Hubble and Spitzer Space Telescope (SST) imaging of SN 2008S and NGC 300 2008OT-1, the prototypes of a common class of stellar transients whose true nature is debated. Both objects are still fading and are now >15 times fainter than their progenitors in the mid-IR and are undetected in the optical and near-IR. Data from the Large Binocular Telescope and Magellan show that neither source has been variable in the optical since fading in 2010. We present models of surviving sources obscured by dusty shells or winds and find that extreme dust models are needed for surviving stars to be successfully hidden by dust, which suggests that these transients may be genuine, but low-energy, supernova explosions. Though SN 2008S is not detected in Chandra X-Ray Observatory data taken in 2012, the flux limits allow the fading IR source to be powered solely by the shock interaction of ejecta with the circumstellar medium if the shock velocity at the time of the observation was ≳20 per cent slower than estimated from emission line widths while the transient was still optically bright. Continued SST monitoring and 10-20 μm observations with James Webb Space Telescope can resolve any remaining ambiguities.

  1. The luminous red nova M101-OT2015-1: a candidate for common envelope ejection

    NASA Astrophysics Data System (ADS)

    Blagorodnova, Nadejda; Kasliwal, Mansi M.; Kotak, Rubina

    2017-01-01

    Binary interaction is an important phase in the study of stellar evolution. Approximately 50% of O star population live in close binary systems as to allow interaction with the companion. Although massive binary progenitors have been associated with thermonuclear supernovae, stripped core collapse supernovae, cataclysmic variables, X-ray binaries, or the mind blowing massive binary black holes recently detected by LIGO, the exact evolutionary path followed by the system is still under debate. One of the critical phases is the common envelope (CE) phase, required to bring a long period binary into a much shorter orbit. Currently, this phase also represents a challenge for the current stellar evolution models. Given the uncertainty, observational constraints are valuable input to advance in this field. One particular class of transient objects, called Luminous Red Novae (LRNe), has been associated with the termination of the CE phase, when a total or partial ejection of the least bound layers of the primary star are expelled at the expense of decreasing the orbital energy of the system. In my talk I will discuss the results of 16 years of observations of M101-OT2015-1, a LRN in M101 galaxy. I will describe the progenitor star (system) and the main characteristics of the outburst. Finally, I will present the results of the evolution of its remnant in infrared wavelengths. Given the long time span of our observations, this event represents one of the best studied CE ejection candidate at extragalactic distances.

  2. Partial KCNQ1OT1 hypomethylation: A disguised familial Beckwith–Wiedemann syndrome as a sporadic adrenocortical tumor

    PubMed Central

    H'mida Ben-Brahim, Dorra; Hammami, Sabeur; Haddaji Mastouri, Marwa; Trabelsi, Saoussen; Chourabi, Maroua; Sassi, Sihem; Mougou, Soumaya; Gribaa, Moez; Zakhama, Abdelfattah; Guédiche, Mohamed Neji; Saad, Ali

    2014-01-01

    Beckwith–Wiedemann syndrome has a wide spectrum of complications such as embryonal tumors, namely adrenocortical tumor. Tumor predisposition is one of the most challenging manifestations of this syndrome. A 45-day old female with a family history of adrenocortical tumor presented with adrenocortical tumor. The case raised suspicion of a hereditary Beckwith–Wiedemann syndrome, therefore molecular analysis was undertaken. The results revealed partial KCNQ1OT1 hypomethylation in the infant's blood DNA which was associated with a complete loss of methylation in the infant's adrenocortical tumor tissue. It is unique for familial Beckwith–Wiedemann syndrome caused by KCNQ1OT1 partial hypomethylation to manifest solely through adrenocortical tumor. Incomplete penetrance and specific tissue mosaicism could provide explanations to this novel hereditary Beckwith–Wiedemann syndrome presentation. PMID:26937341

  3. MASTER OT J130845.02-323254.9: Variable Stars as Source of the High Energy Neutrino.

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Tyurina, N.; Gorbovskoy, E.; Buckley, D.

    2016-09-01

    As reported in ATel #9425 Global MASTER Net auto-detection system ( ( Lipunov et al., MASTER Global Robotic Net, Advances in Astronomy, 2010, 30L) discovered OT source at (RA, Dec) = 13h 08m 45.02s -32d 32m 54.9s on 2016-08-24.73811 UT during inspection of HESE IceCube alert (14 August 2016, 58537957 trigger number http://gcn.gsfc.nasa.gov/notices_amon/58537957_128340.amon , Dornic et al. ATEL #9440 ). MASTER-SAAO auto-detection system detected again OT at RA (2000) = 13 08 45.02 -32 32 54.9 on 2016-09-04.7627UT (ATEL #9425).

  4. Limited Production (LP) Precision Runway Monitor (PRM) Operational Test and Evaluation integration and OT and E Operational Test Plan

    NASA Astrophysics Data System (ADS)

    Livings, Jeffrey

    1995-05-01

    This document defines the Test Plan and corresponding Test Verification Requirements Traceability Matrix (TVRTM) that will be used to conduct the Limited Production (LP) Precision Runway Monitor (PRM) Operational Test and Evaluation (OT and E) Integration and OT and E Operational tests. These tests will be conducted at the Minneapolis-St. Paul International Airport following the Contractor Site Acceptance Test. The LP PRM OT and E test effort will concentrate on Operational Effectiveness and Suitability. The Operational Effectiveness Test consists of a review of the contractor performed Development Test and Evaluation (DT and E) and Site Acceptance Tests. This review will evaluate whether each of the Measures of Effectiveness had been satisfactorily tested and whether the results meet the Minimum Acceptable Operational REquirements MAORs). This review will be conducted solely by test engineers and does not require the PRM system. The Operational Suitability Tests will expose the test participants (Air Traffic (AT) Controllers and Airway Facilities (AF) Technicians) to the PRM system in an operational environment while they perform specified operational procedures. These tests will be conducted in two separate phases: AT Suitability and AF Suitability. Each of these phases is focused on the specific test participants.

  5. Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels

    PubMed Central

    Jeon, Seong Gak; Kim, Kyoung Ah; Chung, Hyunju; Choi, Junghyun; Song, Eun Ji; Han, Seung-Yun; Oh, Myung Sook; Park, Jong Hwan; Kim, Jin-il; Moon, Minho

    2016-01-01

    Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic. PMID:27432189

  6. Impaired Memory in OT-II Transgenic Mice Is Associated with Decreased Adult Hippocampal Neurogenesis Possibly Induced by Alteration in Th2 Cytokine Levels.

    PubMed

    Jeon, Seong Gak; Kim, Kyoung Ah; Chung, Hyunju; Choi, Junghyun; Song, Eun Ji; Han, Seung-Yun; Oh, Myung Sook; Park, Jong Hwan; Kim, Jin-Il; Moon, Minho

    2016-08-31

    Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal neurogenesis and memory are not fully understood. Here, we investigated whether and how the mice with restricted CD4+ repertoire TCR exhibit learning and memory impairment by using OT-II mice. OT-II mice showed decreased adult neurogenesis in hippocampus and short- and long- term memory impairment. Moreover, Th2 cytokines in OT-II mice are significantly increased in peripheral organs and IL-4 is significantly increased in brain. Finally, IL-4 treatment significantly inhibited the proliferation of cultured adult rat hippocampal neural stem cells. Taken together, abnormal level of Th2 cytokines can lead memory dysfunction via impaired adult neurogenesis in OT-II transgenic.

  7. Molecular simulation study of water mobility in aerosol-OT reverse micelles.

    PubMed

    Chowdhary, Janamejaya; Ladanyi, Branka M

    2011-06-16

    In this work, we present results from molecular dynamics simulations on the single-molecule relaxation of water within reverse micelles (RMs) of different sizes formed by the surfactant aerosol-OT (AOT, sodium bis(2-ethylhexyl)sulfosuccinate) in isooctane. Results are presented for RM water content w(0) = [H(2)O]/[AOT] in the range from 2.0 to 7.5. We show that translational diffusion of water within the RM can, to a good approximation, be decoupled from the translation of the RM through the isooctane solvent. Water translational mobility within the RM is restricted by the water pool dimensions, and thus, the water mean-squared displacements (MSDs) level off in time. Comparison with models of diffusion in confined geometries shows that a version of the Gaussian confinement model with a biexponential decay of correlations provides a good fit to the MSDs, while a model of free diffusion within a sphere agrees less well with simulation results. We find that the local diffusivity is considerably reduced in the interfacial region, especially as w(0) decreases. Molecular orientational relaxation is monitored by examining the behavior of OH and dipole vectors. For both vectors, orientational relaxation slows down close to the interface and as w(0) decreases. For the OH vector, reorientation is strongly affected by the presence of charged species at the RM interface and these effects are especially pronounced for water molecules hydrogen-bonded to surfactant sites that serve as hydrogen-bond acceptors. For the dipole vector, orientational relaxation near the interface slows down more than that for the OH vector due mainly to the influence of ion-dipole interactions with the sodium counterions. We investigate water OH and dipole reorientation mechanisms by studying the w(0) and interfacial shell dependence of orientational time correlations for different Legendre polynomial orders.

  8. Unique effects of aerosol OT lamellar structures on the dynamics of guest molecules.

    PubMed

    De, Dipanwita; Datta, Anindya

    2013-06-25

    The behavior of lamellar structures of Aerosol OT (AOT) as hosts, vis-à-vis the flexible normal micelles and rigid nanochannels of Nafion membranes, has been investigated with two different fluorophores, [2,2'-bipyridyl]-3,3'-diol (BP(OH)2) and coumarin 102 (C102). Surprisingly, for BP(OH)2, a rise time is observed at intermediate emission wavelengths and not in the red edge of the fluorescence spectrum. A shoulder at 525 nm is observed in time resolved emission spectra (TRES) at initial times of BP(OH)2 in AOT lamellar structures. This feature is the signature of the monoketo (MK) tautomer, observed for the first time in a microheterogeneous medium. Also, the usually ultrafast single proton transfer in BP(OH)2 is retarded to an considerable extent in lamellar structures. The potential of this medium in promoting unusual intermediates is thus highlighted. This property may be ascribed to the rigidity of lamellar structures, compared to hosts such as regular micelles. However, studies using another fluorophore, coumarin 102 (C102), brings out the fact that these structures are significantly different from the rigid host, Nafion, as well. The absence of excited state proton transfer (ESPT) in this molecule in AOT lamellar structures indicates that it is not protonated, unlike in Nafion. Thus, the interfacial pH of lamellar structures is found to be significantly greater than that of Nafion nanochannels. From the time dependent Stokes shift (TDSS) of the emission spectra of C102, the relaxation time (0.85 ns) of interfacial water in lamellar structures is found to be an order of magnitude faster than that observed in Nafion nanochannels, in which H3O(+) ions have been substituted by different cations. Hence, this study demonstrates that AOT lamellar structures are rather unique hosts and that they behave very differently from conventional rigid and flexible hosts such as normal micelles and Nafion, respectively.

  9. Knockdown of Long Non-Coding RNA KCNQ1OT1 Restrained Glioma Cells’ Malignancy by Activating miR-370/CCNE2 Axis

    PubMed Central

    Gong, Wei; Zheng, Jian; Liu, Xiaobai; Liu, Yunhui; Guo, Junqing; Gao, Yana; Tao, Wei; Chen, Jiajia; Li, Zhiqing; Ma, Jun; Xue, Yixue

    2017-01-01

    Accumulating evidence has highlighted the potential role of long non-coding RNAs (lncRNAs) as biomarkers and therapeutic targets in solid tumors. Here, we elucidated the function and possible molecular mechanisms of lncRNA KCNQ1OT1 in human glioma U87 and U251 cells. Quantitative Real-Time polymerase chain reaction (qRT-PCR) demonstrated that KCNQ1OT1 expression was up-regulated in glioma tissues and cells. Knockdown of KCNQ1OT1 exerted tumor-suppressive function in glioma cells. Moreover, a binding region was confirmed between KCNQ1OT1 and miR-370 by dual-luciferase assays. qRT-PCR showed that miR-370 was down-regulated in human glioma tissue and cells. In addition, restoration of miR-370 exerted tumor-suppressive function via inhibiting cell proliferation, migration and invasion, while promoting the apoptosis of human glioma cells. Knockdown of KCNQ1OT1 decreased the expression level of Cyclin E2 (CCNE2) by binding to miR-370. Further, miR-370 bound to CCNE2 3′UTR region and decreased the expression of CCNE2. These results provided a comprehensive analysis of KCNQ1OT1-miR-370-CCNE2 axis in human glioma cells and might provide a novel strategy for glioma treatment. PMID:28381990

  10. Fluorescent behavior of B-phycoerythrin in microemulsions of aerosol OT/water/isooctane.

    PubMed

    Bermejo, Ruperto; Tobaruela, Diego J; Talavera, Eva M; Orte, Angel; Alvarez-Pez, Jose M

    2003-07-15

    Taking advantage of its unusual fluorescent properties, the incorporation of B-phycoerythrin (B-PE) in aerosol OT (AOT, sodium bis-(2-ethylhexyl) sulphosuccinate)/water/isooctane microemulsions was investigated by following their steady-state and time-resolved fluorescence as a function of the water-to-surfactant molar ratio, w(0). The fluorescent intensity at 575 nm increased continuously with increasing water content, saturating at a w(0) around 35 and staying practically constant at w(0)> or =40. The steady-state anisotropy showed an initial increase with increasing water content until w(0)=23 and then decreased strongly, staying practically constant when w(0)> or =40. The values of the fluorescent parameters, anisotropy and fluorescent intensity, were unchanged when the water content of the system increased in the range between w(0)=40 to 50. This implies the effective incorporation of B-PE in the microemulsion droplets with w(0)> or =40, as well as the equilibrium of the dispersion at these water/surfactant ratios, since higher water content does not affect the main surrounding microenvironment of the protein. The overall incorporation in the microemulsion droplets caused minor spectroscopic changes with respect to biliprotein in aqueous solution of 20 mM sodium phosphate buffer, pH 7.0, such as a blue absorption shift of 3 nm and an emission shift of 1.5 nm, as well as a slight increase in excitation anisotropy spectrum mainly caused by a decrease in protein mobility. Therefore, there are no important interactions between the chromophores and the AOT sulfonate head groups. Emission intensity decays followed complex kinetics in both aqueous and dispersion media. The stability with time and temperature of the biliprotein in the microemulsion was higher than in the aqueous solution. All the results can be explained in terms of B-PE inclusion in the water droplets of AOT microemulsions where the protein has similar configuration and conformation to that in

  11. Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3.

    PubMed

    Terada, Atsushi; Honda, Takashi; Fukuhara, Hideo; Hada, Kazumasa; Kimura, Makoto

    2006-08-01

    Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5'-leader sequence of precursor tRNA (pre-tRNA). Our earlier study revealed that RNase P RNA (pRNA) and five proteins (PhoPop5, PhoRpp38, PhoRpp21, PhoRpp29, and PhoRpp30) in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 reconstituted RNase P activity that exhibits enzymatic properties like those of the authentic enzyme. In present study, we investigated involvement of the individual proteins in RNase P activity. Two particles (R-3Ps), in which pRNA was mixed with three proteins, PhoPop5, PhoRpp30, and PhoRpp38 or PhoPop5, PhoRpp30, and PhoRpp21 showed a detectable RNase P activity, and five reconstituted particles (R-4Ps) composed of pRNA and four proteins exhibited RNase P activity, albeit at reduced level compared to that of the reconstituted particle (R-5P) composed of pRNA and five proteins. Time-course analysis of the RNase P activities of R-4Ps indicated that the R-4Ps lacking PhoPop5, PhoRpp21, or PhoRpp30 had virtually reduced activity, while omission of PhoRpp29 or PhoRpp38 had a slight effect on the activity. The results indicate that the proteins contribute to RNase P activity in order of PhoPop5 > PhoRpp30 > PhoRpp21 > PhoRpp29 > PhoRpp38. It was further found that R-4Ps showed a characteristic Mg2+ ion dependency approximately identical to that of R-5P. However, R-4Ps had optimum temperature of around at 55 degrees C which is lower than 70 degrees C for R-5P. Together, it is suggested that the P. horikoshii RNase P proteins are predominantly involved in optimization of the pRNA conformation, though they are individually dispensable for RNase P activity in vitro.

  12. OT2_eegami_6: SPIRE Snapshot Survey II: Using SPT/CODEX Massive Clusters as Powerful Gravitational Lenses

    NASA Astrophysics Data System (ADS)

    Egami, E.

    2011-09-01

    On the extragalactic side, one of the most remarkable results coming out of Herschel is the discovery of extremely bright (>100 mJy in the SPIRE bands) gravitationally lensed galaxies. The great sensitivity and mapping speed of SPIRE have enabled us to find these rare extraordinary objects. What is truly exciting about these bright lensed galaxies is that they enable a variety of detailed multi-wavelength follow-up observations, shedding new light on the physical properties of these high-redshift sources. In this regard, our OT1 program, "SPIRE Snapshot Survey of Massive Galaxy Clusters" turned out to be a great success. After imaging ~50 galaxies out of 279 in the program, we have already found two spectacularly bright lensed galaxies, one of which is at a redshift of 4.69. This type of cluster-lensed sources are not only bright but also spatially stretched over a large scale, so ALMA (or NOEMA in the north) is likely to be able to study them at the level of individual GMCs. Such studies will open up a new frontier in the study of high-redshift galaxies. Here, we propose to extend this highly efficient and effective survey of gravitationally lensed galaxies to another 353 clusters carefully chosen from the SPT and CODEX cluster samples. These samples contain newly discovered high-redshift (z>0.3) massive (>3-4e14 Msun) clusters, which can be used as powerful gravitational lenses to magnify sources at high redshift. With the OT1 and OT2 surveys together, we expect to find ~20 highly magnified SPIRE sources with exceptional brightnesses (assuming a discovery rate of ~1/30). Such a unique sample of extraordinary objects will enable a variety of follow-up sciences, and will therefore remain as a great legacy of the Herschel mission for years to come.

  13. Purification, crystallization and preliminary crystallographic analysis of RecA superfamily ATPase PH0284 from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Kunishima, Naoki

    2006-04-01

    RecA superfamily ATPase PH0284 from P. horikoshii OT3 was overexpressed, purified, crystallized and cocrystallized with ATP. Both crystal forms belong to the trigonal space group P3{sub 2}21 and diffract X-rays to 2.0 and 2.3 Å resolution, respectively. Circadian (daily) protein clocks are found in cyanobacteria, where a complex of the KaiA, KaiB and KaiC proteins generates circadian rhythms. The 28.09 kDa KaiC homologue PH0284 protein from Pyrococcus horikoshii OT3 was cloned and expressed and the purified protein was crystallized by the oil-microbatch method at 295 K. X-ray diffraction data from the crystal were collected to 2.0 Å resolution using synchrotron radiation at 100 K. The crystal belongs to the trigonal space group P3{sub 2}21, with unit-cell parameters a = b = 96.06, c = 298.90 Å. Assuming the presence of one hexamer in the asymmetric unit gives a V{sub M} value of 2.36 Å{sup 3} Da{sup −1} and a solvent content of 47.9%. A cocrystal with ATP was prepared and a diffraction data set was collected at 2.3 Å resolution.

  14. Mechanistic basis for high reactivity of (salen)Co-OTs in the hydrolytic kinetic resolution of terminal epoxides.

    PubMed

    Nielsen, Lars P C; Zuend, Stephan J; Ford, David D; Jacobsen, Eric N

    2012-03-02

    The (salen)Co(III)-catalyzed hydrolytic kinetic resolution (HKR) of terminal epoxides is a bimetallic process with a rate controlled by partitioning between a nucleophilic (salen)Co-OH catalyst and a Lewis acidic (salen)Co-X catalyst. The commonly used (salen)Co-OAc and (salen)Co-Cl precatalysts undergo complete and irreversible counterion addition to epoxide during the course of the epoxide hydrolysis reaction, resulting in quantitative formation of weakly Lewis acidic (salen)Co-OH and severely diminished reaction rates in the late stages of HKR reactions. In contrast, (salen)Co-OTs maintains high reactivity over the entire course of HKR reactions. We describe here an investigation of catalyst partitioning with different (salen)Co-X precatalysts and demonstrate that counterion addition to epoxide is reversible in the case of the (salen)Co-OTs. This reversible counterion addition results in stable partitioning between nucleophilic and Lewis acidic catalyst species, allowing highly efficient catalysis throughout the course of the HKR reaction.

  15. Mechanistic Basis for High Reactivity of (salen)Co–OTs in the Hydrolytic Kinetic Resolution of Terminal Epoxides

    PubMed Central

    Nielsen, Lars P. C.; Zuend, Stephan J.; Ford, David D.; Jacobsen, Eric N.

    2012-01-01

    The (salen)Co(III)-catalyzed hydrolytic kinetic resolution (HKR) of terminal epoxides is a bimetallic process with a rate controlled by partitioning between a nucleophilic (salen)Co–OH catalyst and a Lewis acidic (salen)Co–X catalyst. The commonly used (salen)Co–OAc and (salen)Co–Cl precatalysts undergo complete and irreversible counterion addition to epoxide during the course of the epoxide hydrolysis reaction, resulting in quantitative formation of weakly Lewis acidic (salen)Co–OH, and severely diminished reaction rates in the late stages of HKR reactions. In contrast, (salen)Co–OTs maintains high reactivity over the entire course of HKR reactions. We describe here an investigation of catalyst partitioning with different (salen)Co–X precatalysts, and demonstrate that counterion addition to epoxide is reversible in the case of the (salen)Co–OTs. This reversible counterion addition results in stable partitioning between nucleophilic and Lewis acidic catalyst species, allowing highly efficient catalysis throughout the course of the HKR reaction. PMID:22292515

  16. Purification, crystallization and initial X-ray crystallographic analysis of the putative GTPase PH0525 from Pyrococcus horikoshii OT3

    SciTech Connect

    Lokanath, Neratur K.; Yamamoto, Hitoshi; Matsunaga, Emiko; Sugahara, Mitsuaki; Kunishima, Naoki

    2005-10-01

    The putative GTPase PH0525 from P. horikoshii OT3 was crystallized using the microbatch method. Crystals were formed under two different conditions, providing two distinct crystal forms. Diffraction data from the two forms were measured to resolution limits of 2.30 and 2.40 Å and processed in space groups P2{sub 1} and C222{sub 1}, respectively. GTPases are involved in diverse cellular functions including cell proliferation, cytoskeleton organization and intracellular traffic. The putative GTPase PH0525 from Pyrococcus horikoshii OT3 has been overexpressed in Escherichia coli and purified. Two distinct crystal forms were grown by the microbatch method at 291 K using a very high protein concentration (80 mg ml{sup −1}). Native data sets extending to resolutions of 2.3 and 2.4 Å have been collected and processed in space groups P2{sub 1} and C222{sub 1}, respectively. Assuming the presence of one monomer per asymmetric unit gives V{sub M} values of 2.6 and 2.4 Å{sup 3} Da{sup −1} for the P2{sub 1} and C222{sub 1} forms, respectively, which is consistent with dynamic light-scattering experiments, which show a monomeric state of the protein in solution.

  17. NGC 300 OT2008-1 as a Scaled-down Version of the Eta Carinae Great Eruption

    NASA Astrophysics Data System (ADS)

    Kashi, Amit; Frankowski, Adam; Soker, Noam

    2010-01-01

    We propose that the intermediate luminosity optical transient NGC 300 OT2008-1 was powered by a mass transfer episode from an extreme asymptotic giant branch star to a main sequence (MS) companion. We find a remarkable similarity in the shapes of the light curves of the several months long NGC 300 OT2008-1 outburst, of the three-month long 2002 enigmatic outburst of the B star V838 Mon, and the twenty-year long Great Eruption of the massive binary system Eta Carinae that occurred in the 19th century. Their similar decline properties hint to a common energy source: a gravitational energy that is released by accretion onto an MS star. These events populate a specific strip in the total energy versus outburst duration diagram. The strip is located between novae and supernovae. We add recent transient events to that diagram and find them to occupy the same strip. This suggests that some intermediate luminosity optical transients are powered by accretion onto a compact object (not necessarily an MS star). These transients are expected to produce bipolar ejecta as a result of the geometry of the accretion process.

  18. Overproduction and preliminary crystallographic study of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3

    SciTech Connect

    Chon, Hyongi; Matsumura, Hiroyoshi; Shimizu, Shoko; Maeda, Nao; Koga, Yuichi; Takano, Kazufumi; Kanaya, Shigenori

    2005-03-01

    A human kynurenine aminotransferase II homologue from P. horikoshii OT3 has been overproduced in E. coli, purified, and characterized. Crystals of this protein have been obtained and analyzed by X-ray diffraction. The Pyrococcus horikoshii OT3 genome contains a gene encoding a human kynurenine aminotransferase II (KAT II) homologue, which consists of 428 amino-acid residues and shows an amino-acid sequence identity of 30% to human KAT II. This gene was overexpressed in Escherichia coli and the recombinant protein (Ph-KAT II) was purified. Gel-filtration chromatography showed that Ph-KAT II exists as a homodimer. Ph-KAT II exhibited enzymatic activity that catalyzes the transamination of l-kynurenine to produce kynurenic acid. Crystals of Ph-KAT II were grown using the sitting-drop vapour-diffusion method and native X-ray diffraction data were collected to 2.2 Å resolution using synchrotron radiation from station BL44XU at SPring-8. The crystals belong to the centred orthorhombic space group C222{sub 1}, with unit-cell parameters a = 71.75, b = 86.84, c = 137.30 Å. Assuming one molecule per asymmetric unit, the V{sub M} value was 2.19 Å{sup 3} Da{sup −1} and the solvent content was 43.3%.

  19. Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model.

    PubMed

    Galvan, Daniel L; O'Neil, Richard T; Foster, Aaron E; Huye, Leslie; Bear, Adham; Rooney, Cliona M; Wilson, Matthew H

    2015-01-01

    Adoptive transfer of gene modified T cells provides possible immunotherapy for patients with cancers refractory to other treatments. We have previously used the non-viral piggyBac transposon system to gene modify human T cells for potential immunotherapy. However, these previous studies utilized adoptive transfer of modified human T cells to target cancer xenografts in highly immunodeficient (NOD-SCID) mice that do not recapitulate an intact immune system. Currently, only viral vectors have shown efficacy in permanently gene-modifying mouse T cells for immunotherapy applications. Therefore, we sought to determine if piggyBac could effectively gene modify mouse T cells to target cancer cells in a mouse cancer model. We first demonstrated that we could gene modify cells to express murine interleukin-12 (p35/p40 mIL-12), a transgene with proven efficacy in melanoma immunotherapy. The OT-I melanoma mouse model provides a well-established T cell mediated immune response to ovalbumin (OVA) positive B16 melanoma cells. B16/OVA melanoma cells were implanted in wild type C57Bl6 mice. Mouse splenocytes were isolated from C57Bl6 OT-I mice and were gene modified using piggyBac to express luciferase. Adoptive transfer of luciferase-modified OT-I splenocytes demonstrated homing to B16/OVA melanoma tumors in vivo. We next gene-modified OT-I cells to express mIL-12. Adoptive transfer of mIL-12-modified mouse OT-I splenocytes delayed B16/OVA melanoma tumor growth in vivo compared to control OT-I splenocytes and improved mouse survival. Our results demonstrate that the piggyBac transposon system can be used to gene modify splenocytes and mouse T cells for evaluating adoptive immunotherapy strategies in immunocompetent mouse tumor models that may more directly mimic immunotherapy applications in humans.

  20. Data Multiplexing Network (DMN), phase 3: Equipment Operational Test and Evaluation (OT&E)/Integration test plan

    NASA Astrophysics Data System (ADS)

    Bell, Wayne

    1991-10-01

    The presented plan addresses the Operational Test and Evaluation (OT&E)/Integration testing of the Data Multiplexing Network (DMN) equipment in the current NAS environment. The approach and concept is to conduct integration tests with appropriate National Airspace System (NAS) systems to verify that NAS requirements and DMN specifications and Statistical Multiplexer requirements were satisfied. This will be accomplished by conducting unit level tests at the Federal Aviation Administration (FAA) Technical Center and extensive integration tests at the key test sites of Albuquerque, New Mexico; Denver, Colorado; and Minneapolis, Minnesota Air Route Traffic Control Centers (ARTCC). The DMN equipment being procured includes Deterministic Time Division Multiplexing (DTDM) Commercial Off-The-Shelf (COTS) equipment and the NON-COTS equipment (a/b switch and clock box). This plan describes the test program for the DTDM COTS equipment.

  1. Synthesis and Characterization of MO[OSi(OtBu)3]4 andMO2[OSi(OtBu)3]2 (M=Mo,W): Models for Isolated Oxo-Molybdenum and-Tungsten Sites on Silica and Precursors to Molybdena- and Tungsta-SilicaMaterials

    SciTech Connect

    Jarupatrakorn, Jonggol; Coles, Martyn P.; Tilley, T. Don

    2005-01-20

    The tri(alkoxy)siloxy complexes MO[OSi(OtBu)3]4 (1, M = Mo and 2, M = W) were prepared from MOCl4 and LiOSi(OtBu)3. Similarly, reactions of MO2Cl2(DME) with LiOSi(OtBu)3 afforded the new siloxide complexes MO2[OSi(OtBu)3]2 (3, M = Mo and 4, M = W), which are thermally unstable at ambient temperature. More stable compounds were obtained by the crystallizations of 3 and 4 in a coordinating solvent, to form the ether adducts MoO2[OSi(OtBu)3]2(THF) (3a) and WO2[OSi(OtBu)3]2(DME) (4a). These compounds serve as soluble models for isolated molybdenum or tungsten atoms on a silica surface and were characterized by 1H, 13C, 29Si, 95Mo, and 183W NMR, FT-Raman, FT-IR, and UV-vis spectroscopies. Compounds 1, 2, 3a, and 4a were used to prepare metal-oxide silica composites via the thermolytic molecular precursor method. The xerogels obtained from the thermolyses of 1, 2, 3a, and 4a in toluene contained mesoporosity with surface areas of 10, 230, 106, and 270 m2 g-1, respectively. Despite the high surface areas for most samples, these xerogels contain MO3 domains. Complexes 1 and 2 were also used to introduce molybdenum and tungsten sites, respectively, onto mesoporous SBA-15 silica via displacement of the -OSi(OtBu)3 ligand for a siloxyl group from the silica surface. All molybdenum- and tungsten-containing systems were tested as catalysts for the epoxidation of cyclohexene using tert-butyl hydroperoxide (TBHP) or aqueous H2O2 as the oxidant.

  2. A Genome-wide Association Study of Bipolar Disorder with Comorbid Eating Disorder Replicates the SOX2-OT Region

    PubMed Central

    Liu, Xiaohua; Kelsoe, John R.; Greenwood, Tiffany A.

    2015-01-01

    Background Bipolar disorder is a heterogeneous mood disorder associated with several important clinical comorbidities, such as eating disorders. This clinical heterogeneity complicates the identification of genetic variants contributing to bipolar susceptibility. Here we investigate comorbidity of eating disorders as a subphenotype of bipolar disorder to identify genetic variation that is common and unique to both disorders. Methods We performed a genome-wide association analysis contrasting 184 bipolar subjects with eating disorder comorbidity against both 1,370 controls and 2,006 subjects with bipolar disorder only from the Bipolar Genome Study (BiGS). Results The most significant genome-wide finding was observed bipolar with comorbid eating disorder vs. controls within SOX2-OT (p=8.9×10−8 for rs4854912) with a secondary peak in the adjacent FXR1 gene (p=1.2×10−6 for rs1805576) on chromosome 3q26.33. This region was also the most prominent finding in the case-only analysis (p=3.5×10−7 and 4.3×10−6, respectively). Several regions of interest containing genes involved in neurodevelopment and neuroprotection processes were also identified. Limitations While our primary finding did not quite reach genome-wide significance, likely due to the relatively limited sample size, these results can be viewed as a replication of a recent study of eating disorders in a large cohort. Conclusions These findings replicate the prior association of SOX2-OT with eating disorders and broadly support the involvement of neurodevelopmental/neuroprotective mechanisms in the pathophysiology of both disorders. They further suggest that different clinical manifestations of bipolar disorder may reflect differential genetic contributions and argue for the utility of clinical subphenotypes in identifying additional molecular pathways leading to illness. PMID:26433762

  3. Self-assembly of new surface active ionic liquids based on Aerosol-OT in aqueous media.

    PubMed

    Rao, K Srinivasa; Gehlot, Praveen Singh; Trivedi, Tushar J; Kumar, Arvind

    2014-08-15

    New anionic ionic liquid surfactants have been synthesized by replacing the sodium cation of Aerosol-OT (sodium dioctylsulfosuccinate, [Na]AOT) with various biocompatible moieties, such as 1-butyl-3-methyl imidazolium ([C4mim]), proliniumisopropylester ([ProC3]), cholinium ([Cho]), and guanidinium ([Gua]). The Aerosol-OT derived ionic liquids (AOT-ILs) were found fairly soluble in water and formed vesicles above a critical vesicle concentration (CVC) which depended upon the nature of cation, and followed the order: [ProC3]<[C4mim]<[Gua]<[Cho]

  4. Glucuronidation of OTS167 in Humans Is Catalyzed by UDP-Glucuronosyltransferases UGT1A1, UGT1A3, UGT1A8, and UGT1A10

    PubMed Central

    Ramírez, Jacqueline; Mirkov, Snezana; House, Larry K.

    2015-01-01

    OTS167 is a potent maternal embryonic leucine zipper kinase inhibitor undergoing clinical testing as antineoplastic agent. We aimed to identify the UDP-glucuronosyltransferases (UGTs) involved in OTS167 metabolism, study the relationship between UGT genetic polymorphisms and hepatic OTS167 glucuronidation, and investigate the inhibitory potential of OTS167 on UGTs. Formation of a single OTS167-glucuronide (OTS167-G) was observed in pooled human liver (HLM) (Km = 3.4 ± 0.2 µM), intestinal microsomes (HIM) (Km = 1.7 ± 0.1 µM), and UGTs. UGT1A1 (64 µl/min/mg) and UGT1A8 (72 µl/min/mg) exhibited the highest intrinsic clearances (CLint) for OTS167, followed by UGT1A3 (51 µl/min/mg) and UGT1A10 (47 µl/min/mg); UGT1A9 was a minor contributor. OTS167 glucuronidation in HLM was highly correlated with thyroxine glucuronidation (r = 0.91, P < 0.0001), SN-38 glucuronidation (r = 0.79, P < 0.0001), and UGT1A1 mRNA (r = 0.72, P < 0.0001). Nilotinib (UGT1A1 inhibitor) and emodin (UGT1A8 and UGT1A10 inhibitor) exhibited the highest inhibitory effects on OTS167-G formation in HLM (68%) and HIM (47%). We hypothesize that OTS167-G is an N-glucuronide according to mass spectrometry. A significant association was found between rs6706232 and reduced OTS167-G formation (P = 0.03). No or weak UGT inhibition (range: 0–21%) was observed using clinically relevant OTS167 concentrations (0.4–2 µM). We conclude that UGT1A1 and UGT1A3 are the main UGTs responsible for hepatic formation of OTS167-G. Intestinal UGT1A1, UGT1A8, and UGT1A10 may contribute to first-pass OTS167 metabolism after oral administration. PMID:25870101

  5. /sup 45/Ca efflux for myometrial cells: comparison of the effects of prostaglandin F/sub 2/. cap alpha. (PGF/sub 2/), oxytocin (OT) and arachidonate (A)

    SciTech Connect

    Katona, G.; Molnar, M.; Toth, M.; Hertelendy, F.

    1986-03-01

    The aim of this study was to measure PGF/sub 2..cap alpha../-induced Ca/sup 2 +/ release from uterine cells and to compare this to the actions of OT and A. Smooth muscle cells isolated from the uterus (shell gland) of laying hens were cultured for 7 days in M199 plus 10% fetal calf serum. The cells were treated with digitonin (20..mu..M) and preloaded with /sup 45/Ca for 40 min. Addition of PGF/sub 2..cap alpha../ caused a biphasic /sup 45/Ca-efflux. There was a small but significant /sup 45/Ca-release within 30 sec (rapid phase) followed by a larger one within 7 min (slow phase). In comparison, both OT and A stimulated /sup 45/Ca efflux during a single, slow phase. The maximal effect of A was observed at < 7 min, whereas that of OT was slower, peaking after 7 min. Mepacrin, an inhibitor of A release, attenuated the action of OT without having any effect on A promoted /sup 45/Ca-efflux. Indomethacin, an inhibitor of PG synthase, failed to suppress the Ca-releasing effect of A suggesting the A itself or a lipoxygenase product may have been responsible for the observed effects. Moreover, these results provide suggestive evidence that A release is an important step in the action of various uterotonic agents converging on the mobilization of intracellular Ca.

  6. Down-Regulatory Effects of miR-211 on Long Non-Coding RNA SOX2OT and SOX2 Genes in Esophageal Squamous Cell Carcinoma

    PubMed Central

    Shafiee, Mohammad; Aleyasin, Seyed Ahmad; Vasei, Mohammad; Semnani, Shahriar Semnani; Mowla, Seyed Javad

    2016-01-01

    Objective MicroRNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that tran- scriptionally or post-transcriptionally regulate gene expression through degradation of their mRNA targets and/or translational suppression. However, there are a few reports on miRNA-mediated expression regulation of long ncRNAs (lncRNAs). We have previ- ously reported a significant upregulation of the lncRNA SOX2OT and its intronic cod- ing gene, SOX2, in esophageal squamous cell carcinoma (ESCC) tissue samples. In this study, we aimed to evaluate the effect of induced overexpression of miR-211 on SOX2OT and SOX2 expression in vitro. Materials and Methods In this experimental study, we performed both bioinformatic and experimental analyses to examine whether these transcripts are regulated by miRNAs. From the list of potential candidate miRNAs, miR-211 was found to have complementary sequences to SOX2OT and SOX2 transcripts. To validate our finding experimentally, we transfected the NT-2 pluripotent cell line (an embryonal carcinoma stem cell) with an expression vector overexpressing miR-211. The expression chang- es of miR-211, SOX2OT, and SOX2 were then quantified by a real-time polymerase chain reaction (RT-PCR) approach. Results Compared with mock-transfected cells, overexpression of miR-211 caused a significant down-regulation of both genes (P<0.05). Furthermore, flow-cytometry analysis revealed a significant elevation in sub-G1 cell population following ectopic expression of miR-211 in NT-2 cells. Conclusion We report here, for the first time, the down-regulation of SOX2OT and SOX2 genes by an miRNA. Considering the vital role of SOX2OT and SOX2 genes in pluripotency and tumorigenesis, our data suggest an important and inhibitory role for miR-211 in the aforementioned processes. PMID:26862518

  7. The influence of polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT) and its metabolite-dichlorodiphenyldichloroethylene (DDE) on mRNA expression for NP-I/OT and PGA, involved in oxytocin synthesis in bovine granulosa and luteal cells.

    PubMed

    Mlynarczuk, Jaroslaw; Wrobel, Michal H; Kotwica, Jan

    2009-11-01

    The effect of polychlorinated biphenyls (PCBs) congeners (PCB 77, PCB 126, PCB 153) and their technical mixture-Aroclor (Ar) 1248, as well as dichlorodiphenyltrichloroethane (DDT) and its metabolite-dichlorodiphenyldichloroethylene (DDE; two individual isomers p,p'- and o,p'- or their mixture, 95% and 5%, respectively) at the dose of 10 ng/ml each, on the gene expression of (a) oxytocin (OT) precursor-neurophysin-oxytocin (NP-I/OT) and (b) peptidyl glycine-alpha-amidating mono-oxygenase (PGA), the terminal enzyme in the pathway of OT synthesis, was studied. Granulosa cells from follicles >1cm in diameter, collected on days 19-21 of estrous cycle, and luteal cells from corpora lutea (CL) collected on days 8-12 of the estrous cycle were used. The cells were incubated (6h) with these xenobiotics and the expression of NP-I/OT and PGA genes was determined. All PCBs increased (P<0.05) NP-I/OT gene expression in granulosa cells. Similarly, all PCBs but PCB 126 increased (P<0.05) PGA gene expression in these cells. DDT and DDE increased (P<0.05) gene expression of NP-I/OT in granulosa cells, while gene expression of PGA in these cells was stimulated (P<0.05) by DDE only. The mRNA expression for NP-I/OT and PGA in luteal cells was increased (P<0.05) by PCB 77 and PCB 153. Both DDE isomers and mixture also stimulated (P<0.05) of NP-I/OT mRNA expression, while increase (P<0.05) of PGA mRNA expression was elicited by incubation of these cells with DDE mixture and Ar 1248. Obtained data suggest that PCBs, DDT and DDE can affect the mRNA expression for NP-I/OT and PGA in bovine granulosa and luteal cells.

  8. Characterization of the PH1704 protease from Pyrococcus horikoshii OT3 and the critical functions of Tyr120.

    PubMed

    Zhan, Dongling; Bai, Aixi; Yu, Lei; Han, Weiwei; Feng, Yan

    2014-01-01

    The PH1704 protease from hyperthermophilic archaean Pyrococcus horikoshii OT3 is a member of DJ-1/ThiJ/PfpI superfamily with diverse functional subclasses. The recombinant PH1704 was efficiently purified and was systematically characterized by a combination of substrate specificity analysis, steady-state kinetics study and molecular docking research. The homogeneous protease was obtained as a presumed dodecamer with molecular weight of ∼240 kDa. Iodoacetamide strongly inhibited the peptidase activity, confirming that Cys100 is a nucleophilic residue. The recombinant protein was identified as both an aminopeptidase and an endopeptidase. Experimental data showed that L-R-amc was the best substrate of PH1704. Structural interaction fingerprint analysis (SIFt) indicated the binding pose of PH1704 and showed that Tyr120 is important in substrate binding. Kinetic parameters Kcat and Kcat/Km of the Y120P mutant with L-R-amc was about 7 and 7.8 times higher than that of the wild type (WT). For the endopeptidase Y120P with AAFR-amc, Kcat and Kcat/Km is 10- and 21-fold higher than that of WT. Experimental data indicate the important functions of Tyr120: involvement in enzyme activity to form a hydrogen bond with Cys100 and as an entrance gate of the substrate with Lys43. The results of this study can be used to investigate the DJ-1/ThiJ/PfpI superfamily.

  9. Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway1[OPEN

    PubMed Central

    Liu, Lei; Yan, Xiaojing; Zhao, Yiqiang

    2017-01-01

    The expression of genes with aberrant structure is prevented at both the transcriptional and posttranscriptional regulation levels. Aberrant gene silencing at the posttranscriptional level is well studied; however, it is not well understood how aberrant genes are silenced at the transcriptional level. In this study, through genetic screening a transgenic report line that harbors an aberrant gene (35S-LUC, lacking 3′-untranslated region [3′-UTR]) and lacks luciferase (LUC) activity, we identify that the small ubiquitin-like modifier (SUMO) protease OTS1 gene is required for maintaining the silence of the reporter 35S-LUC and an endogenous mutator-like element MULE-F19G14 at the transcriptional level, which requires DNA-dependent RNA polymerase (Pol) V and DDR complex, but not Pol IV. The increased transcripts in ots1 mutants are terminated by the 3′-UTRs of downstream genes. In addition to ots1 mutations, mutations in several known or putative SUMO proteases and two SUMO E3 ligases, SIZ1 and MMS21, have similar effects on this silencing regulation. Taken together, our results reveal that the enzymes involved in the SUMOylation process restrain aberrant gene transcription by using a downstream gene 3′-UTR, and this regulation requires a functional Pol V-dependent pathway in Arabidopsis (Arabidopsis thaliana). PMID:27852949

  10. Perovskite LaFeO3 nanoparticles synthesized by the reverse microemulsion nanoreactors in the presence of aerosol-OT: Morphology, crystal structure, and their optical properties

    NASA Astrophysics Data System (ADS)

    Abazari, Reza; Sanati, Soheila

    2013-12-01

    Orthorhombic structure of lanthanum ferrite nanoparticles (LaFeO3 NPs) with perovskite type phase has been synthesized with water-in-oil (W/O) microemulsion consisted of water/dioctyl sulfosuccinate sodium (aerosol-OT)/isooctane at room temperature. It has been shown that aerosol-OT reverse microemulsion solution is appropriate for synthesizing perovskite LaFeO3 NPs in the absence of any co-surfactants. Field emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), energy dispersive analysis of X-ray (EDAX), X-ray diffraction (XRD), ultraviolet-visible absorption spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FT-IR) have been adopted for characterization of surface morphology, size, phase composition, structure, and optical properties of the considered NPs. Furthermore, the optical properties of LaFeO3 NPs have been further analyzed via photoluminescence (PL) spectroscopy. As shown by the physicochemical characterizations, our prepared NPs via aerosol-OT reverse microemulsion solution are spherical and nearly uniform (with a size of about 24.65 nm). Besides, they include an orthorhombic phase while no impurities are observed. Single phase lanthanum ferrite NPs have successfully been prepared at 500 °C. Moreover, UV-Vis spectrum indicates that the LaFeO3 NPs synthesized through this technique can be considered as a type of photo-catalytic materials.

  11. Synthesis and Characterization of Et2NM OB[Si(OtBu)3]2 3 (M=Zr,Hf) Molecular Precursors to Zr/B/Si/O and Hf/B/Si/O Materials

    SciTech Connect

    Fujdala, Kyle L.; Tilley, T. Don

    2005-03-18

    Two new transition metal complexes containing the -OB[OSi(OtBu)3]2 ligand, Et2NM{l_brace}OB[OSi(OtBu)3]2{r_brace}3 (M = Zr, Hf), are reported. These species are the 4th and 5th examples of complexes containing this recently reported borosiloxide ligand. Their use as potential molecular precursors to multi-component oxide materials via the thermolytic molecular precursor (TMP) method is discussed.

  12. Characterisation of microstructures formed in isopropyl palmitate/water/Aerosol OT:1-butanol (2:1) system.

    PubMed

    Boonme, P; Krauel, K; Graf, A; Rades, T; Junyaprasert, V B

    2006-11-01

    The aim of this work was to determine the type and microstructure of microemulsion samples formed in IPP/water/AerosolOT:1-butanol (2:1) systems as a case study for the investigation of microemulsions. The concentration of the surfactant/cosurfactant mixture was kept constant while the ratio of water to oil was varied. Several techniques were used to investigate the types and phase transitions of the microemulsion formulations. The experimental methods used included visual observation cross-polarized light microscopy (PLM) appearance, conductivity, viscosity, cryo-field emission scanning electron microscopy (cryo-FESEM), differential scanning calorimetry (DSC), nuclear magnetic resonance (NMR), and fluorescence resonance energy transfer (FRET). Taken together, the results of the various techniques imply that the systems investigated are undergoing two transitions as a function of water concentration. Between 10-15%w/w of water, the systems change from headgroup hydrated surfactant solutions in oil (or possibly very small reversed micellar systems) to w/o microemulsions. These systems then change to o/w microemulsions between 25-30%w/w of water. The transitions however, appear to be gradual, as for example the DSC data indicates a transition between 15-20%w/w of water. Furthermore, for some methods the changes observed were very weak, and only with supportive data of other techniques can the phase behaviour of the microemulsion systems be interpreted with confidence. Interestingly, no indication of the presence of a bicontinuous intermediate microstructure was found. Liquid crystal formation was detected in samples containing 55%w/w of water.

  13. Simultaneous Photometry and Spectroscopy of the Deeply Absorbing Polar MASTER OT J132104.04+560957.8

    NASA Astrophysics Data System (ADS)

    Hoyt, Taylor; Littlefield, Colin; Garnavich, Peter M.

    2016-01-01

    We present photometry and time-resolved spectroscopy of the suspected polar MASTER OT J132104.04+560957.8 (J1321) taken at the Large Binocular Telescope (LBT) simultaneously with the LBC camera and MODS spectrograph in binocular mode. Our V-band photometry confirmed a primary and secondary maximum first identified by Littlefield et al. In the spectra we see unusually deep Balmer and helium absorption features for about 10-15% of a 91 minute orbital period. The absorption lines first appear to the red of the emission peaks then shift bluewards, ultimately dominating the line profile at zero radial velocity. Towards the end of the absorption phase, weak absorption reappears to the blue of the emission lines. When the system is bright the spectra show a broadly-peaked continuum with a maximum around 500nm. We do not see absorption during the secondary bright red phase. Around the minima the continuum is blue and resembles a more typical polar spectrum with strong Balmer, neutral helium and ionized helium emission lines. The Doppler tomograms of J1321 are fuzzy and blobby and resemble those of the asynchronous polar BY Cam. The highly variable width of the secondary maximum remains a mystery along with the lack of a discernible irradiated secondary in the tomograms. We use our observations to constrain the properties of the white dwarf's magnetic field as well as the accretion flow structure. We also discuss evidence of J1321 being in a prolonged low-mass-transfer state in all observations prior to 2007.

  14. A dimeric molecular precursor [(tBuO)2Ti mu-O2Si[OSi(OtBu)3]2]2to Ti(IV)/SiO2 catalysts for selective cyclohexene epoxidation

    SciTech Connect

    Brutchey, Richard L.; Mork, Benjamin V.; Sirbuly, Donald J.; Yang, Peidong; Tilley, T. Don

    2005-04-20

    The new dimeric complex [(tBuO)2Ti({mu}-O2Si[OSi(OtBu)3]2)]2 (1), prepared via silanolysis of Ti(OtBu)4 with (HO)2Si[OSi(OtBu)3]2, is a structural and spectroscopic (NMR, FT-IR, UV-vis, XPS) model for Ti(IV)/SiO2. The molecular complex was used to prepare titanium-containing silica materials through both the thermolytic molecular precursor method (yielding TiO2{center_dot}3SiO2) or by grafting 1 onto mesoporous SBA-15 silica. Grafting 1 onto SBA-15 yields mostly isolated Ti(IV) sites, as evidenced by DRUV-vis and photoluminescence spectroscopies. The resulting materials were found to be active and highly selective in the epoxidation of cyclohexene, yielding up to 71% of cyclohexene oxide based on oxidant (cumene hydroperoxide) after 2 h at 65 C in toluene.

  15. Growth of a radiation-transformed clone of C3H 1OT1/2 cells into melanin-producing colonies

    SciTech Connect

    Szekely, J.G.; Raaphorst, G.P.; Lobreau, A.U.; Azzam, E.I.; Vadasz, J.A.

    1985-01-01

    When R25, a radiation-transformed clone of C3H 1OT1/2 cells, was plated in soft agarose, a fraction of the colonies became pigmented. The morphologies of the white and dark colonies and their cells were compared by optical, transmission and scanning electron microscopy. The transformed R25 cells had apparently differentiated into melanin-producing cells in the soft agarose, with the white colonies containing actively growing cells having only a few melanosomes, and the dark colonies being made up of stationary-phase cells filled with electronopaque melanosomes. Exposure of the R25 cells to 4.0 Gy of X-rays decreased the percentage of dark colonies, while exposure to 1% DMSO had no effect.

  16. FLOYDS Classification of MASTER OT J161308.18+260953.3 as a Type II Supernova and ASASSN-17cq/AT 2017axl as a Type Ia Supernova Near Peak

    NASA Astrophysics Data System (ADS)

    Hosseinzadeh, G.; Arcavi, I.; Howell, D. A.; McCully, C.; Valenti, S.

    2017-02-01

    We obtained a spectrum of MASTER OT J161308.18+260953.3 (ATel #10099) on 2017 February 21.5 UT with the robotic FLOYDS instrument mounted on the Las Cumbres Observatory (LCO) 2-meter telescope on Haleakala, Hawai'i.

  17. Discovery of an unusual bright eclipsing binary with the longest known period: TYC 2505-672-1/MASTER OT J095310.04+335352.8

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Gorbovskoy, E.; Afanasiev, V.; Tatarnikova, A.; Denisenko, D.; Makarov, D.; Tiurina, N.; Krushinsky, V.; Vinokurov, A.; Balanutsa, P.; Kuznetsov, A.; Gress, O.; Sergienko, Yu.; Yurkov, V.; Gabovich, A.; Tlatov, A.; Senik, V.; Vladimirov, V.; Popova, E.

    2016-04-01

    We report on the MASTER Global Robotic Net discovery of an eclipsing binary, MASTER OT J095310.04+335352.8, previously known as unremarkable star TYC 2505-672-1, which displays extreme orbital parameters. The orbital period P = 69.1 yr is more than 2.5 times longer than that of ɛ-Aurigae, which is the previous record holder. The light curve is characterized by an extremely deep total eclipse with a depth of more than 4.5 mag, which is symmetrically shaped and has a total duration of 3.5 yr. The eclipse is essentially gray. The spectra acquired with the Russian 6 m BTA telescope both at minimum and maximum light mainly correspond to an M0-1III-type red giant, but the spectra taken at the bottom of eclipse show small traces of a sufficiently hot source. The observed properties of this system can be better explained as the red giant eclipsed by a large cloud (the disk) of small particles surrounding the invisible secondary companion.

  18. Neutron and X-Ray Reflectivity Studies of the Adsorption of Aerosol-OT at the Air-Water Interface: The Structure of the Calcium Salt

    PubMed

    Li; Lee; Thomas; Penfold

    1997-03-15

    We have used neutron and X-ray reflection to determine the structure of a layer of calcium bis-(2-ethyl 1-hexyl) sulphosuccinate (Aerosol-OT or AOT) adsorbed at the air/solution interface. The widths of the distributions of the chains and head groups of the molecule, and their positions in relation to the underlying water, have been measured at four concentrations varying from the solubility limit (CMC) at 4 x 10(-4) M to 1 x 10(-6) M. Over this concentration range the coverage changes from 68 ± 3 to 142 ± 8 Å2 per AOT unit. The structure of the layer both is quite different from that of NaAOT and varies quite differently with surface concentration. The Ca(AOT)2 layer is slightly (1 Å) further out from the water, but the chain region is thinner for the calcium surfactant. This is reflected most in the greatly reduced chain to head separation, which drops from about 6 Å in NaAOT to about 4 Å in Ca(AOT)2.

  19. EPA/ORD/RTP (Environmental Protection Agency, Office of Research and Development, Research Triangle Park) asbestos program support for the OTS (Office of Toxic Substances) asbestos-in-schools program. Final report

    SciTech Connect

    Beard, M.E.

    1986-03-01

    An overview of the EPA/ORD/RTP Asbestos program support for the OTS Asbestos-in-Schools Program is given. The program includes (1) a bulk sample asbestos analysis audit program, (2) an intercomparison of three measurement methods for airborne asbestos, (3) the development of audit materials for airborne asbestos measurement methods, and (4) the development of guidance for post-abatement asbestos air monitoring.

  20. Evaluation of the efficiency of tumor and tissue delivery of carrier-mediated agents (CMA) and small molecule (SM) agents in mice using a novel pharmacokinetic (PK) metric: relative distribution index over time (RDI-OT)

    NASA Astrophysics Data System (ADS)

    Madden, Andrew J.; Rawal, Sumit; Sandison, Katie; Schell, Ryan; Schorzman, Allison; Deal, Allison; Feng, Lan; Ma, Ping; Mumper, Russell; DeSimone, Joseph; Zamboni, William C.

    2014-11-01

    The pharmacokinetics (PK) of carrier-mediated agents (CMA) is dependent upon the carrier system. As a result, CMA PK differs greatly from the PK of small molecule (SM) drugs. Advantages of CMAs over SMs include prolonged circulation time in plasma, increased delivery to tumors, increased antitumor response, and decreased toxicity. In theory, CMAs provide greater tumor drug delivery than SMs due to their prolonged plasma circulation time. We sought to create a novel PK metric to evaluate the efficiency of tumor and tissue delivery of CMAs and SMs. We conducted a study evaluating the plasma, tumor, liver, and spleen PK of CMAs and SMs in mice bearing subcutaneous flank tumors using standard PK parameters and a novel PK metric entitled relative distribution over time (RDI-OT), which measures efficiency of delivery. RDI-OT is defined as the ratio of tissue drug concentration to plasma drug concentration at each time point. The standard concentration versus time area under the curve values (AUC) of CMAs were higher in all tissues and plasma compared with SMs. However, 8 of 17 SMs had greater tumor RDI-OT AUC0-last values than their CMA comparators and all SMs had greater tumor RDI-OT AUC0-6 h values than their CMA comparators. Our results indicate that in mice bearing flank tumor xenografts, SMs distribute into tumor more efficiently than CMAs. Further research in additional tumor models that may more closely resemble tumors seen in patients is needed to determine if our results are consistent in different model systems.

  1. Behavioral responses to pups in males with different reproductive experiences are associated with changes in central OT, TH and OTR, D1R, D2R mRNA expression in mandarin voles.

    PubMed

    Wang, Bo; Li, Yani; Wu, Ruiyong; Zhang, Shuwei; Tai, Fadao

    2015-01-01

    Male rodents behave differently toward pups because of different sexual and/or paternal experiences; however, the mechanisms underlying these responses are not well understood. Using socially monogamous mandarin voles (Microtus mandarinus) we investigated the behavioral responses of males with different reproductive experiences (virgin males, paired males and new fathers) to new born pups. Central levels of neuropeptide oxytocin (OT), tyrosine hydroxylase (TH), as well as oxytocin receptor (OTR), dopamine 1-type receptor (D1R) and dopamine 2-type receptor (D2R) mRNA expression in the nucleus accumbens and medial amygdala were also measured in these males. Our data showed that new fathers exhibited more approaching behavior and contained more OT-immunoreactive and TH-immunoreactive neurons. In addition to increased OTR mRNA expression in the nucleus accumbens and medial amygdala, new fathers had higher D1R and D2R mRNA expression in the nucleus accumbens, and less D1R and D2R mRNA expression in the medial amygdala than paired males. These results demonstrate that males with different reproductive experiences display different behavioral responses to pups and that these differences are associated with altered OT and dopamine, and their receptors in specific brain regions.

  2. OT2_sserje01_2: THE HERSCHEL-AKARI NEP DEEP SURVEY: the cosmological history of stellar mass assembly and black hole accretion

    NASA Astrophysics Data System (ADS)

    Serjeant, S.

    2011-09-01

    We propose a far-IR and submm mapping survey of the premier AKARI deep field in the North Ecliptic Pole, in PACS/SPIRE parallel mode. This is the only major deep infrared field not yet covered by Herschel guaranteed or open time key projects. The outstanding and unparalleled continuous mid-IR photometric coverage from AKARI, far better than equivalent Spitzer surveys, enables a wide range of galaxy evolution diagnostics unachievable in any other survey field (including Herschel HerMES/PEP fields), by spanning the wavelengths of redshifted PAH and silicate features and the peak energy output of AGN dust tori. The investment by AKARI in the NEP represents ~10 percent of the entire pointed observations available throughout the lifetime of AKARI. Our proposal remedies the remarkable omission from Herschel's legacy surveys of the premier extragalactic deep field from another IR space telescope. We will simultaneously identify and find photometric redshifts for the Herschel point source population, make stacking analysis detections of the galaxies which dominate the submm extragalactic background light as a function of redshift, determine the bolometric power outputs of the galaxies that dominate the submm background, compare the UV/optical/mid-IR continuum/PAH/far-IR/submm/radio star formation rate estimator in the most comprehensive IR survey data set to date, and track the coupled stellar mass assembly and black hole accretion throughout most of the history of the Universe. In OT1 the HOTAC concluded "The science output from the proposed survey will be outstanding [...] The panel was convinced that these observations should be done" but it since became clear that priority 2 time is very unlikely to be executed, so we request reclassification to priority 1.

  3. Ries Bunte Breccia revisited: Indications for the presence of water in Itzing and Otting drill cores and implications for the emplacement process

    NASA Astrophysics Data System (ADS)

    Pietrek, Alexa; Kenkmann, Thomas

    2016-07-01

    We reassessed two drill cores of the Bunte Breccia deposits of the Ries crater, Germany. The objectives of our study were the documentation of evidence for water in the Bunte Breccia, the evaluation of how that water influenced the emplacement processes, and from which preimpact water reservoir it was derived. The Bunte Breccia in both cores can be structured into a basal layer composed mainly of local substrate material, overlain by texturally and compositionally diverse, crater-derived breccia units. The basal layer is composed of the youngest sediments (Tertiary clays and Upper Jurassic limestone) and has a razor-sharp boundary to the upper breccia units, which are composed of older rocks of Upper Jurassic to Upper Triassic age. Sparse material exchange occurred between the basal layer and the rest of the Bunte Breccia. Fluids predominantly came from the Tertiary and the Upper Triassic sandstone formation. In the basal layer, Tertiary clays were subjected to intense, ductile deformation, indicating saturation with water. This suggests that water was mixed into the matrix, creating a fluidized basal layer with a strong shear localization. In the upper units, Upper Triassic sandstones are intensely deformed by granular flow. The texture requires that the rocks were disaggregated into granular sand. Vaporization of pore water probably aided fragmentation of these rocks. In the Otting core, hot suevite (T > 600 °C) covered the Bunte Breccia shortly after its emplacement. Vertically oriented gas escape pipes in suevite partly emanate directly at the contact to the Bunte Breccia. They indicate that the Bunte Breccia contained a substantial amount of water in the upper part that was vaporized and escaped through these vents.

  4. Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids.

    PubMed

    Kawakami, Ryushi; Ohmori, Taketo; Sakuraba, Haruhiko; Ohshima, Toshihisa

    2015-08-01

    To date, there have been few reports analyzing the amino acid requirement for growth of hyperthermophilic archaea. We here found that the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 requires Thr, Leu, Val, Phe, Tyr, Trp, His and Arg in the medium for growth, and shows slow growth in medium lacking Met or Ile. This largely corresponds to the presence, or absence, of genes related to amino acid biosynthesis in its genome, though there are exceptions. The amino acid requirements were dramatically lost by addition of D-isomers of Met, Leu, Val, allo-Ile, Phe, Tyr, Trp and Arg. Tracer analysis using (14)C-labeled D-Trp showed that D-Trp in the medium was used as a protein component in the cells, suggesting the presence of D-amino acid metabolic enzymes. Pyridoxal 5'-phosphate (PLP)-dependent racemase activity toward Met, Leu and Phe was detected in crude extract of P. horikoshii and was enhanced in cells grown in the medium supplemented with D-amino acids, especially D-allo-Ile. The gene encoding the racemase was narrowed down to one open reading frame on the basis of enzyme purification from P. horikoshii cells, and the recombinant enzyme exhibited PLP-dependent racemase activity toward several amino acids, including Met, Leu and Phe, but not Pro, Asp or Glu. This is the first report showing the presence in a hyperthermophilic archaeon of a PLP-dependent amino acid racemase with broad substrate specificity that is likely responsible for utilization of D-amino acids for growth.

  5. Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3

    SciTech Connect

    Bagautdinov, Bagautdin; Matsuura, Yoshinori; Bagautdinova, Svetlana; Kunishima, Naoki

    2007-04-01

    A truncated form of biotin carboxyl carrier protein containing the C-terminal half fragment (BCCPΔN76) and the biotin protein ligase (BPL) with the mutation R48A (BPL*) or the double mutation R48A K111A (BPL**) were successfully cocrystallized in the presence of ATP and biotin. The BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals belong to space group P2{sub 1} and diffract X-rays to 2.7 and 2.0 Å resolution, respectively. Biotin protein ligase (BPL) catalyses the biotinylation of the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase. To elucidate the exact details of the protein–protein interactions in the biotinylation function, the C-terminal half fragment of BCCP (BCCPΔN76), the R48A mutant of BPL (BPL*) and the R48A K111A double mutant of BPL (BPL**), all of which are from Pyrococcus horikoshii OT3, have been expressed, purified and successfully cocrystallized. Cocrystals of the BPL*–BCCPΔN76 and BPL**–BCCPΔN76 complexes as well as crystals of BPL*, BPL** and BCCPΔN76 were obtained by the oil-microbatch method using PEG 20 000 as a precipitant at 295 K. Complete X-ray diffraction data sets for BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals were collected at 100 K to 2.7 and 2.0 Å resolution, respectively, using synchrotron radiation. They belong to the monoclinic space group P2{sub 1}, with similar unit-cell parameters a = 69.85, b = 63.12, c = 75.64 Å, β = 95.9°. Assuming two subunits of the complex per asymmetric unit gives a V{sub M} value of 2.45 Å{sup 3} Da{sup −1} and a solvent content of 50%.

  6. Results of investigations conducted in the LaRC 4-foot unitary plan wind tunnel leg no. 1 using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA94A)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Aero-loads investigations were conducted on the updated configuration-5 space shuttle launch vehicle at Mach numbers 2.50, 3.50, and 4.50. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested, with two different forward orbiter-to-external-tank attach-strut configurations. The entire vehicle model 72-OTS was supported by means of a balance mounted in the orbiter through its base and suspended from an appropriate sting for the specific tunnel.

  7. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (41-OTS) in the Langley Research Center unitary plan wind tunnel at M equals 3.7 (IH16)

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1975-01-01

    The results are presented of supersonic heat transfer tests performed on the .006 scale space shuttle vehicle model (41-OTS) in the Langley Research Center Unitary Plan Wind Tunnel. These tests were conducted to parametrically investigate ascent heating of the integrated vehicle and its components. The tests were conducted at a nominal Mach number of 3.7 and Reynolds numbers per foot of 2 and 5 million. The model configurations investigated were the integrated vehicle and each component alone (i.e. orbiter, tank and SRB). All the configurations were run with and without transition strips and through an angle of attack range of 0 deg to minus 5 deg with the exception of the SRB which was tested through an angle of attack range of minus 5 deg to 90 deg. The heat transfer data were obtained from 223 iron constantan thermocouples attached to stainless steel thin-skin areas of the model.

  8. Aerodynamic results of a separation effects test on a 0.010-scale model (52-OTS) of the integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A (IA111), volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1976-01-01

    Graphical data obtained during experimental wind tunnel aerodynamic investigations of a 0.010 scale model (52-OTS) of the integrated space shuttle vehicle was presented. The purpose of this investigation was to obtain data with the solid rocket booster (SRB) in proximity to the orbiter/external tank (O/ET), over a large O/ET initial angle of attack and sideslip range, as well as data on the SRB alone (greatly separated from the O/ET). A captive trajectory system, which supported the SRB, was used with the tunnel primary sector (supporting the O/ET) to obtain grid type separation effects data. One symmetrical SRB model was used interchangeably to obtain right-hand and left-hand SRB data. The entire investigation was conducted at a free-stream Mach number of 4.5 at unit Reynolds number of 3.95 and 5.9 million per foot.

  9. An experimental determination in Calspan Ludwieg tube of the base environment of the integrated space shuttle vehicle at simulated Mach 4.5 flight conditions (test IH5 of model 19-OTS)

    NASA Technical Reports Server (NTRS)

    Drzewiecki, R. F.; Foust, J. W.

    1976-01-01

    A model test program was conducted to determine heat transfer and pressure distributions in the base region of the space shuttle vehicle during simulated launch trajectory conditions of Mach 4.5 and pressure altitudes between 90,000 and 210,000 feet. Model configurations with and without the solid propellant booster rockets were examined to duplicate pre- and post-staging vehicle geometries. Using short duration flow techniques, a tube wind tunnel provided supersonic flow over the model. Simultaneously, combustion generated exhaust products reproduced the gasdynamic and thermochemical structure of the main vehicle engine plumes. Heat transfer and pressure measurements were made at numerous locations on the base surfaces of the 19-OTS space shuttle model with high response instrumentation. In addition, measurements of base recovery temperature were made indirectly by using dual fine wire and resistance thermometers and by extrapolating heat transfer measurements.

  10. Aerodynamic results of a separation effects test on a 0.010-scale model (52-OTS) of the integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A (IA111), volume 2

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1976-01-01

    Tabular data obtained during experimental wind tunnel aerodynamic investigations of a 0.010 scale model (52-OTS) of the integrated space shuttle vehicle was presented. The purpose of this investigation was to obtain data with the solid rocket booster (SRB) in proximity to the orbiter/external tank (O/ET), over a large O/ET initial angle of attack and sideslip range, as well as data on the SRB alone (greatly separated from the O/ET). A captive trajectory system, which supported the SRB, was used with the tunnel primary sector (supporting the O/ET) to obtain grid type separation effects data. One symmetrical SRB model was used interchangeably to obtain right-hand and left-hand SRB data. The entire investigation was conducted at free-stream Mach number of 4.5 at unit Reynolds number of 3.95 and 5.9 million per foot.

  11. Results of an investigation of the space shuttle integrated vehicle aerodynamic heating characteristics obtained using the 0.0175-scale model 60-OTS in AEDC tunnel A during tests IH41 and IH41A

    NASA Technical Reports Server (NTRS)

    Cummings, J. W.; Dye, W. H.

    1977-01-01

    A thin skin thermocouple test was conducted to obtain heat-transfer data on the space shuttle integrated vehicle during the ascent phase of the flight profile. The test model was the 0.0175-scale thin skin thermocouple model (60-OTS) of the Rockwell International vehicle 5 configuration. The test was conducted at nominal Mach numbers of 2.5, 3.5, 4.5, and 5.5, and a free stream unit Reynolds number of 5 million per ft. Heat transfer data were obtained for angles of attack of 0, + or - 5, and 10 deg and yaw angles of 0, 3, and 6 deg. The integrated vehicle model was tested with the external tank configured with both a smooth ogive nose and an ogive nose with a spherical nose tip (nipple nose). The remainder of the test was conducted with the external tank installed alone in the tunnel.

  12. Investigations of the 0.020-scale 88-OTS Integrated Space Shuttle Vehicle Jet-Plume Model in the NASA/Ames Research Center 11 by11-Foot Unitary Plan Wind Tunnel (IA80). Volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    The results are documented of jet plume effects wind tunnel test of the 0.020-scale 88-OTS launch configuration space shuttle vehicle model in the 11 x 11 foot leg of the NASA/Ames Research Center Unitary Plan Wind Tunnel. This test involved cold gas main propulsion system (MPS) and solid rocket motor (SRB) plume simulations at Mach numbers from 0.6 to 1.4. Integrated vehicle surface pressure distributions, elevon and rudder hinge moments, and wing and vertical tail root bending and torsional moments due to MPS and SRB plume interactions were determined. Nozzle power conditions were controlled per pretest nozzle calibrations. Model angle of attack was varied from -4 deg to +4 deg; model angle of sideslip was varied from -4 deg to +4 deg. Reynolds number was varied for certain test conditions and configurations, with the nominal freestream total pressure being 14.69 psia. Plotted force and pressure data are presented.

  13. Expression of escherichia coli otsA in a Saccharomyces cerevisiae tps1 mutant restores trehalose 6-phosphate levels and partly restores growth and fermentation with glucose and control of glucose influx into glycolysis.

    PubMed

    Bonini, B M; Van Vaeck, C; Larsson, C; Gustafsson, L; Ma, P; Winderickx, J; Van Dijck, P; Thevelein, J M

    2000-08-15

    The TPS1 gene, encoding trehalose-6-phosphate synthase (TPS), exerts an essential control on the influx of glucose into glycolysis in the yeast Saccharomyces cerevisiae. The deletion of TPS1 causes an inability to grow on glucose because of a hyperaccumulation of sugar phosphates and depletion of ATP and phosphate. We show that expression of the Escherichia coli homologue, otsA, in a yeast tps1 mutant results in high TPS activity. Although the trehalose 6-phosphate (Tre6P) level during exponential growth on glucose was at least as high as in a wild-type yeast strain, growth on glucose was only partly restored and the lag phase was much longer. Measurement of the glycolytic metabolites immediately after the addition of glucose showed that in spite of a normal Tre6P accumulation there was still a partial hyperaccumulation of sugar phosphates. Strong elevation of the Tre6P level by the additional deletion of the TPS2 gene, which encodes Tre6P phosphatase, was not able to cause a strong decrease in the sugar phosphate levels in comparison with the wild-type strain. In addition, in chemostat experiments the short-term response to a glucose pulse was delayed, but normal metabolism was regained over a longer period. These results show that Tre6P synthesis from a heterologous TPS enzyme can to some extent restore the control of glucose influx into glycolysis and growth on glucose in yeast. However, they also indicate that the yeast TPS enzyme, as opposed to the E. coli otsA gene product, is able to increase the efficiency of the Tre6P control on glucose influx into yeast glycolysis.

  14. Expression of KCNQ1OT1, CDKN1C, H19, and PLAGL1 and the methylation patterns at the KvDMR1 and H19/IGF2 imprinting control regions is conserved between human and bovine

    PubMed Central

    2012-01-01

    Background Beckwith-Wiedemann syndrome (BWS) is a loss-of-imprinting pediatric overgrowth syndrome. The primary features of BWS include macrosomia, macroglossia, and abdominal wall defects. Secondary features that are frequently observed in BWS patients are hypoglycemia, nevus flammeus, polyhydramnios, visceromegaly, hemihyperplasia, cardiac malformations, and difficulty breathing. BWS is speculated to occur primarily as the result of the misregulation of imprinted genes associated with two clusters on chromosome 11p15.5, namely the KvDMR1 and H19/IGF2. A similar overgrowth phenotype is observed in bovine and ovine as a result of embryo culture. In ruminants this syndrome is known as large offspring syndrome (LOS). The phenotypes associated with LOS are increased birth weight, visceromegaly, skeletal defects, hypoglycemia, polyhydramnios, and breathing difficulties. Even though phenotypic similarities exist between the two syndromes, whether the two syndromes are epigenetically similar is unknown. In this study we use control Bos taurus indicus X Bos taurus taurus F1 hybrid bovine concepti to characterize baseline imprinted gene expression and DNA methylation status of imprinted domains known to be misregulated in BWS. This work is intended to be the first step in a series of experiments aimed at determining if LOS will serve as an appropriate animal model to study BWS. Results The use of F1 B. t. indicus x B. t. taurus tissues provided us with a tool to unequivocally determine imprinted status of the regions of interest in our study. We found that imprinting is conserved between the bovine and human in imprinted genes known to be associated with BWS. KCNQ1OT1 and PLAGL1 were paternally-expressed while CDKN1C and H19 were maternally-expressed in B. t. indicus x B. t. taurus F1 concepti. We also show that in bovids, differential methylation exists at the KvDMR1 and H19/IGF2 ICRs. Conclusions Based on these findings we conclude that the imprinted gene expression of

  15. Aerodynamic results of a separation effects test on a 0.01-scale model (52-OTS) of integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A, volume 1

    NASA Technical Reports Server (NTRS)

    Campbell, J. H., II

    1975-01-01

    Experimental aerodynamic investigations were conducted, during the period July 18-19, 1974, in the AEDC/VKF Tunnel A facility on a 0.01-scale model (52-OTS) of the integrated space shuttle vehicle, including only one SRB. The purpose of the investigation was to obtain data for close-in proximity (SRB to orbiter/tank) effects with the orbiter/tank combination at relatively high alpha and beta attitudes, and with the SRB separation motors off. The AEDC Captive Trajectory System (CTS), which supported the SRB, was used in conjunction with the tunnel primary sector (supporting the orbiter/tank) to obtain grid type separation effects data. The one symmetrical SRB model was used interchangeably to obtain both right-hand and left-hand SRB data. Free-stream data were also obtained for the orbiter/tank and for the SRB. This data was used to provide baselines for proximity effects. The entire investigation was conducted at a free-stream Mach number of 4.5 with unit Reynolds number ranging from 4.0 to 6.5 million per foot.

  16. Results of aeroheating DFI and ET design-data test on a 0.0175-scale model 60-OTS conducted in the Von Karman Gas Dynamics Facility (VKF) 40" supersonic and the 50" hypersonic wind tunnels A and C

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Leef, C. R.

    1985-01-01

    The 0.0175-scale thin-skin thermocouple Model 60-OTS was tested in the von Karman Gas Facility 40-inch continuous flow supersonic tunnel A and the VKF Tunnel C. Testing was conducted at Mach numbers 2.25 to 4.0, and Reynolds numbers from 0.4 x 10 to the 6th power/ft to 6.6 x 10 to the 6th power/ft. Angle of attack range was from -5.0 to +5.0 degrees, and angle of sideslip range was from -6 to +6 degrees. The primary objective of this test was to provide a valid base for the external tank (ET) and solid rocket booster (SRB) heating prediction methodology for ascent flight by taking heating data at Development Flight Instrumentation (DFI) locations for flight conditions simulating STS-1 through -4. A second objective was to obtain additional aeroheating data to support potential reduction of the thermal protection system (TPS) on the ET. The third phase of the test was funded and conducted by NASA/MSFC for the purpose of establishing confidence in the data base from the lower temperature tunnel A.

  17. Place preferences associated with pups or cocaine change the expression of D2R, V1aR and OTR in the NAcc and MeA and the levels of plasma AVP, OT, T and E2 in mandarin vole fathers.

    PubMed

    Fang, Qianqian; Wang, Jianli

    2017-03-04

    Drug abuse often has negative impacts on parenting behavior. The dopamine (DA), arginine vasopressin (AVP) and oxytocin (OT) systems are involved in paternal behavior and drug-induced behaviors. Mandarin voles (Microtus mandarinus) are socially monogamous rodents with high levels of paternal behavior. The aims of this study were to examine the protein expression levels of the DA 2-type receptor (D2R), AVP receptor 1A(V1aR) and OT receptor (OTR) in the nucleus accumbens (NAcc) and medial amygdala (MeA) as well as the plasma hormone responses after mandarin vole fathers were conditioned with their pups or cocaine. Our experimental models are based on the conditioned place preference (CPP) paradigm. We observed CPP in response to either pup- or cocaine-associated cues in the mandarin vole fathers. Fathers that were conditioned to either pups or cocaine had a lower expression of D2R and V1aR in the NAcc than did controls. Fathers that were conditioned to pups had higher levels of OTR expression in the MeA and higher plasma levels of AVP, OT, estradiol (E2), and lower plasma levels of testosterone (T) than did controls. Fathers that were conditioned to cocaine exhibited lower levels of plasma AVP and T. These results indicate that the reward effects of pup and cocaine are both mediated by D2R, V1aR and OTR in the NAcc and MeA and that there are subtle differences between the pup and cocaine reward mechanisms that are associated with altered plasma AVP, OT, T and E2.

  18. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity.

    PubMed

    Rathod, Dnyaneshwar; Golinska, Patrycja; Wypij, Magdalena; Dahm, Hanna; Rai, Mahendra

    2016-10-01

    The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line.

  19. Results of a M = 5.3 heat transfer test of the integrated vehicle using phase-change paint techniques on the 0.0175-scale model 56-OTS in the NASA/Ames Research Center 3.5-foot hypersonic wind tunnel

    NASA Technical Reports Server (NTRS)

    Marroquin, J.

    1985-01-01

    An experimental investigation was performed in the NASA/Ames Research Center 3.5-foot Hypersonic Wind Tunnel to obtain supersonic heat-distribution data in areas between the orbiter and external tank using phase-change paint techniques. The tests used Novamide SSV Model 56-OTS in the first and second-stage ascent configurations. Data were obtained at a nominal Mach number of 5.3 and a Reynolds number per foot of 5 x 10 to the 6th power with angles of attack of 0 deg, +/- 5 deg, and sideslip angles of 0 deg and +/- 5 deg.

  20. Identity of Innovative Multifunctional Material Manufacturing Business in Latvia / Inovatīvu Daudzfunkcionālo Materiālu Ražotāju Identitāte Latvijā

    NASA Astrophysics Data System (ADS)

    Geipele, I.; Staube, T.; Ciemleja, G.; Zeltins, N.; Ekmanis, Yu.

    2015-08-01

    The publication comprises the results from the practical scientific investigation to define the profile and distinctions of the Latvian innovative multifunctional material manufacturing industry. The research is carried out by a holistic approach, including expert interviews, qualitative analysis of the official register data, practical survey, and financial data analysis. The paper seeks to give the detailed data on a technological profile of the mentioned representative companies, if there is a synergy or tough competition in the Latvian market. The current research is topical, because it is unique and does not have analogues in Latvia, and the research is timely due to correspondence with recently stated Research and Innovation Strategies for Smart Specialisation in Latvia. The main findings are associated with recognition of the factors that make impact on commercialisation of the finished goods, obtained financial results and planned directions of development of the respondent companies. Publikācija sniedz veiktā zinātniski praktiskā pētījuma rezultātus, kura ietvaros tika noteikts Latvijas inovatīvu daudzfunkcionālo materiālu ražotāju darbības profils un identitāte. Pētījumā tika izmantota kompleksās analīzes metode, kas ietvēra ekspertu intervijas, uzņēmumu oficiāli reģistrēto datu kvalitatīvo analīzi, zinātniski praktisko aptauju, kā arī pētāmo uzņēmumu finanšu darbības rezultātu analīzi. Zinātniskais darbs sniedz izsmeļošu informāciju par mērķa uzņēmumu tehnoloģisko profilu un pēta, vai Latvijas mērķa ražošanas nozarē ir sinerģijas vai sīvās konkurences pazīmes. Šim zinātniskajam pētījumam ir noteikta aktualitāte saskaņā ar tā unikalitāti Latvijas valsts mērogā un atbilstība Viedās specializācijas stratēģijai. Pētījuma galvenie secinājumi ir saistīti ar uzņēmumu produkcijas komercializācijas ietekmējošo faktoru apzināšanu, sasniegtajiem finansiālajiem rezultātiem un pl

  1. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... company by such savings association, and related applications for permission to organize an interim..., which involve: (A) Fraud, moral turpitude, dishonesty, breach of trust or fiduciary duties, organized crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation...

  2. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... company by such savings association, and related applications for permission to organize an interim..., which involve: (A) Fraud, moral turpitude, dishonesty, breach of trust or fiduciary duties, organized crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation...

  3. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... company by such savings association, and related applications for permission to organize an interim..., which involve: (A) Fraud, moral turpitude, dishonesty, breach of trust or fiduciary duties, organized crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation...

  4. NYPIRG Petion to Object ot Kings Plaza Title V Permit

    EPA Pesticide Factsheets

    This document may be of assistance in applying the Title V air operating permit regulations. This document is part of the Title V Petition Database available at www2.epa.gov/title-v-operating-permits/title-v-petition-database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

  5. OT1_mrubio_1: Clumping in OB-star winds

    NASA Astrophysics Data System (ADS)

    Rubio, M.

    2010-07-01

    Massive stars, their nature and evolution, play a important role at all stages of the Universe. Through their radiatively driven winds they influence on the dynamics and energetics of the interstellar medium. The winds of OB stars are the most studied case. Commonly, the mass-loss rates of luminous OB stars are inferred from several types of measurements, the strengths of UV P Cygni lines, H-alpha emission and radio and FIR continuum emission. Recent evidence indicates that currently accepted mass-loss rates may need to be revised downwards when small-scale density inhomogeneities (clumping) are taken into account. We argue that only a consistent treatment of ALL possible diagnostics, scanning different parts of the winds, and analyzed by means of state of the art model atmospheres, will permit the determination of true mass-loss rates. To this end we have assembled a variety of multi-wavelength data, but one crucial observational set is missing: far-IR diagnostics of free-free emission, which uniquely constrain the clumping properties of the wind at intermediate heights. We propose, therefore, to use PACS photometric mode to fill this crucial gap, studying the 70 and 110 micron fluxes of a carefully selected sample of 29 O4-B8 stars. These observations will provide the missing information to derive the clumping properties of the entire outflow, to understand the wind physics, and to obtain reliable massloss rates.

  6. OT2_mrubio_2: Clumping in OB-star winds

    NASA Astrophysics Data System (ADS)

    Rubio, M.

    2011-09-01

    Massive stars, their nature and evolution, play a important role at all stages of the Universe. Through their radiatively driven winds they influence on the dynamics and energetics of the interstellar medium. The winds of OB stars are the most studied case. Commonly, the mass-loss rates of luminous OB stars are inferred from several types of measurements, the strengths of UV P Cygni lines, H-alpha emission and radio and FIR continuum emission. Recent evidence indicates that currently accepted mass-loss rates may need to be revised downwards when small-scale density inhomogeneities (clumping) are taken into account. We argue that only a consistent treatment of ALL possible diagnostics, scanning different parts of the winds, and analyzed by means of state of the art model atmospheres, will permit the determination of true mass-loss rates. To this end we have assembled a variety of multi-wavelength data, but one crucial observational set is missing: far-IR diagnostics of free-free emission, which uniquely constrain the clumping properties of the wind at intermediate heights. We propose, therefore, to use PACS photometric mode to fill this crucial gap, studying the 70 and 110 micron fluxes of a carefully selected sample of 29 O4-B8 stars. These observations will provide the missing information to derive the clumping properties of the entire outflow, to understand the wind physics, and to obtain reliable massloss rates.

  7. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ACQUISITION OF CONTROL OF... foreign bank is not subject to comprehensive supervision or regulation on a consolidated basis by the...), “comprehensive supervision or regulation on a consolidated basis by the appropriate authorities” shall...

  8. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your...

  9. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your...

  10. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your...

  11. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your...

  12. OTS Selective Bibliography. High Temperature Metallurgy and Heat Resistant Alloys

    DTIC Science & Technology

    1961-02-01

    electrospark machining, explosive cutting, and cent, UHS-260, Halcomb 218, A-286, 410 Stainless, electrolytic machining), by Clifford, Semones and R...OF METALS, by Fassell, Peterson cludes metals - electro- deposition ). Final report, and Chamberlain. Tech. rept. no. III, part I. by Kruh. 1957. 88p...penetration, tion to molybdenum above about 9000 C in air. The crater diameter, and volume of target material dis- .001 inch chromium deposits offer some

  13. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of legal or supervisory issues; (C) The board of directors and executive officers of the holding... the association; (D) The acquisition raises no significant issues of law or policy under then current... frames: (1) On the business day prior to the date of consummation of the acquisition, the chief...

  14. OT1_atielens_1: Physical conditions in PDRs

    NASA Astrophysics Data System (ADS)

    Tielens, A.

    2010-07-01

    Luminous stars have a profound influence on their environment as their far-UV (6-13.6 eV) photons dissociate and ionize surrounding gas. The gas in these so-called PhotoDissociation Regions (PDRs) is heated by the photo-electric effect on polycyclic aromatic hydrocarbon (PAH) molecules and very small grains (VSG) and cools through emission in atomic fine-structure ([OI], [CII], [SiII]) and molecular rotational (H2, CO) lines. PDR models are known to have intrinsic problems and have moreover only been tested using observations with very large beams on complex regions. Herschels high sensitivity, high spatial resolution, and wide wavelength coverage allows for the first time a study of the far-IR spectra of spatially resolved PDRs. We propose to map the key diagnostic, far-IR and sub-mm, atomic ([CII] 158m, [OI] 63, 145m) and molecular (CO) lines using PACS and SPIRE in a sample of edge-on, spatially resolved Galactic PDRs. The sources in this sample are all well-studied over a wide-wavelength range and have all been mapped by IRS/Spitzer in the rotational H2 lines, the [SiII] 34m line, the PAH features and the VSG continuum. The goals of our combined Herschel/Spitzer study are to determine the density and temperature structure of the region, to quantify the gas energetics (cooling/heating efficiency), and to compare the gas (heating) characteristics with the emission characteristics of the (neutral & cationic) PAHs and VSGs. This will provide deep insight in the photo-electric heating of atomic gas which is central to the structure of PDRs, the phases of the ISM, and the structure of protoplanetary disks. In addition, these well-known Galactic PDRs provide natural laboratories for studies of the interaction of massive stars with their environment and a semi-empirical way of calibrating the infrared characteristics of regions of massive star formation and, thus, the observational characteristics of galaxies out to the early Universe.

  15. OT1_dhunter_3: Characterizing Molecular Clouds at Low Metallicity

    NASA Astrophysics Data System (ADS)

    Hunter, D.

    2010-07-01

    Molecular gas is difficult to detect from traditional millimeter CO transitions in dwarf galaxies below a certain metallicity. Yet, there is evidence for lots of molecular H_2 in these galaxies. Fortunately, Photo-dissociation Regions are a better tracer of the molecular material in low metallicity systems. In metal-poor galaxies, PDRs dominate the molecular core where CO is found, and in the current paradigm the PDR grows and the core shrinks as metallicity decreases. Thus, we expect critical differences in the molecular clouds of dwarfs compared to spirals, with the differences becoming more extreme with lower metallicity. Yet, understanding these differences and their consequences to star formation is essential to understanding the processes that drive star formation at low metallicities. Therefore, we propose to observe the PDRs in 5 regions in 5 typical metal-poor dwarf galaxies spanning a range in oxygen abundance. We will use these observations to characterize the molecular gas, examine the correspondence between the molecular clouds and the atomic gas and star formation characteristics, and determine the characteristics of the atomic ISM that are necessary for the formation of these dense molecular clouds. We will also test the molecular cloud structure paradigm as a function of metallicity.

  16. Complete genome sequence of Haliscomenobacter hydrossis type strain (OT)

    SciTech Connect

    Daligault, Hajnalka E.; Lapidus, Alla L.; Zeytun, Ahmet; Nolan, Matt; Lucas, Susan; Glavina Del Rio, Tijana; Tice, Hope; Cheng, Jan-Fang; Tapia, Roxanne; Han, Cliff; Goodwin, Lynne A.; Pitluck, Sam; Liolios, Konstantinos; Pagani, Ioanna; Ivanova, N; Huntemann, Marcel; Mavromatis, K; Mikhailova, Natalia; Pati, Amrita; Chen, Amy; Palaniappan, Krishna; Land, Miriam L; Hauser, Loren John; Brambilla, Evelyne-Marie; Rohde, Manfred; Verbarg, Susanne; Goker, Markus; Bristow, James; Eisen, Jonathan; Markowitz, Victor; Hugenholtz, Philip; Kyrpides, Nikos C; Klenk, Hans-Peter; Woyke, Tanja

    2011-01-01

    Haliscomenobacter hydrossis van Veen et al. 1973 is the type species of the genus Halisco- menobacter, which belongs to order 'Sphingobacteriales'. The species is of interest because of its isolated phylogenetic location in the tree of life, especially the so far genomically un- charted part of it, and because the organism grows in a thin, hardly visible hyaline sheath. Members of the species were isolated from fresh water of lakes and from ditch water. The genome of H. hydrossis is the first completed genome sequence reported from a member of the family 'Saprospiraceae'. The 8,771,651 bp long genome with its three plasmids of 92 kbp, 144 kbp and 164 kbp length contains 6,848 protein-coding and 60 RNA genes, and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

  17. OT2_baltieri_5: Star formation in proto-clusters

    NASA Astrophysics Data System (ADS)

    Altieri, B.

    2011-09-01

    Massive clusters of galaxies have been found to date from as early as 3-4 billion years after the Big Bang. Cosmological simulations using the current cold dark matter model predict that these systems should descend from 'proto-clusters' - early overdensities of massive galaxies that merge hierarchically to form a cluster. These protocluster regions themselves are built up hierarchically and so are expected to contain extremely massive galaxies, progenitors of the quiescent behemoths observed in cores of the present day massive galaxy clusters. Observational evidence for this picture, however, is sparse because high-redshift proto-clusters are rare and difficult to observe. Here we propose to probe with Herschel SPIRE the very beginning of the cluster and massive galaxies formation process by observing 5 proto-clusters at 3

  18. Report: Ozone Transport Commission Incurred Costs Under EPA Assistance Agreements XA98379901, OT83098301, XA97318101, and OT83264901

    EPA Pesticide Factsheets

    Report #2007-4-00068, July 31, 2007. We questioned $2,723,706 of the $9,042,706 in reported outlays because the recipient claimed unallowable outlays for contractual services, indirect costs, and in-kind costs.

  19. (/sup 3/H)-(Thr4,Gly7)OT: a highly selective ligand for central and peripheral OT receptors

    SciTech Connect

    Elands, J.; Barberis, C.; Jard, S.

    1988-01-01

    Oxytocin receptors in rat hippocampal synaptic plasma membranes were compared with mammary gland and uterine oxytocin receptors. For this purpose, a highly specific oxytocic agonist (Thr4,Gly7)oxytocin was tritiated. We demonstrated that this ligand labels oxytocin receptors selectively. Scatchard analyses revealed a high affinity for all the oxytocin receptors investigated, with equilibrium dissociation constants between 1.0 and 2.0 nM. Binding appeared to take place at a single population of receptor sites. Competition experiments confirmed the high affinity of arginine vasopressin for hippocampal oxytocin receptors but also revealed that mammary gland and uterine oxytocin receptors do not discriminate more efficiently between oxytocin and arginine vasopressin. This lack in specificity is not affected by applying different concentrations of Mg ions.

  20. VHF Direction Finder (VDF) Operational Test and Evaluation (OT&E) integration and OT&E Operational Test Report

    DTIC Science & Technology

    1994-05-01

    conducted at three locations on several dates. Testing took place at the FAA Technical Center and the Millville , NJ , AFSS in 1988. Additional testing...lnternational Airport NJ .08405. Thim: dzuzen? ha! Deon capprov~d f 31 p.b1ic rdease and sale: its dia tributiod n b UfiLn t.ed I DTIC QUAL 1Ty I.WJSP pCrEC...U.S. Department of Transportation FederalAviation Administration Technical CenterAtlantic CityAirport, NJ 08405 4V " 1 099 NOTICE This document Is

  1. Dimolybdenum(III) complexes of -OSi(OtBu)3, -O2P(OtBu)2 and-OB[OSi(OtBu)3]2 as single-source molecular precursors tomolybdenum-containing, multicomponent oxide materials

    SciTech Connect

    Fujdala, Kyle L.; Tilley, T. Don

    2004-01-15

    The following dimolybdenum complexes containing -OSi(O{sup t}Bu){sub 3}, -O{sub 2}P(O{sup t}Bu){sub 2}, and -OB[OSi(O{sup t}Bu){sub 3}]{sub 2} ligands have been synthesized and structurally characterized: Mo{sub 2}(NMe{sub 2}){sub 4}[OSi(O{sup t}Bu){sub 3}]{sub 2} (1), Mo{sub 2}(O{sup t}Bu)4[OSi(O{sup t}Bu){sub 3}]{sub 2} (2), Mo{sub 2}(NMe){sub 4}(OB[OSi(O{sup t}Bu){sub 3}]{sub 2}){sub 2} (3), Mo{sub 2}(NMe{sub 2}){sub 2}[-O{sub 2}P(O{sup t}Bu){sub 2}]{sub 2}[O{sub 2}P(O{sup t}Bu){sub 2}]{sub 2} (4), Mo{sub 2}(NMe{sub 2}){sub 2}[OSi(O{sup t}Bu){sub 3}]{sub 2}[-O{sub 2}P(O{sup t}Bu){sub 2}]{sub 2} (5), and Mo{sub 2}(NMe{sub 2}){sub 2}[-O{sub 2}P(O{sup t}Bu){sub 2}]{sub 2}OB[OSi(O{sup t}Bu){sub 3}]{sub 2} (6). The isolation and structural characterization of trans- and cis-isomers of complexes 4 and 5 (4a and 4b, 5a and 5b, respectively) are also reported. Studies of the thermal decompositions of the complexes (by thermogravimetric analysis and solution 1H NMR spectroscopy) were performed. Xerogels with approximate compositions of 2MoO{sub 1.5}{center_dot}2P{sub 2}O{sub 5} and 2MoO{sub 1.5}{center_dot}2P{sub 2}O{sub 5}{center_dot}2SiO{sub 2} were derived from 4a and 5a or 5b, respectively, via solution thermolyses (toluene). The as-synthesized (and dried) xerogels contain one equiv of HNMe{sub 2} per molybdenum center (by combustion analysis, IR spectroscopy, and thermogravimetric analysis), and these materials have high surface areas (up to 270 m{sup 2} g{sup -1}). Upon calcination at 300 C, the coordinated amines are lost and the surface areas are significantly reduced (to 40 m{sup 2} g{sup -1} and <5 m{sup 2} g{sup -1} for the materials derived from 4 and 5a or 5b, respectively). Solid-state {sup 31}P MAS NMR spectroscopy suggests that the as-synthesized xerogels retain structural features of the starting molecular precursors, as indicated by the presence of resonances that correspond to both bridging and terminal -O{sub 2}P(O{sup t}Bu){sub 2} ligands. Upon calcination at 300 C, the resonances for bridging -O{sub 2}P(O{sup t}Bu){sub 2} groups are replaced by those for PO{sub 4}{sup 3-}. The material derived from 4 exhibits low activity and poor selectivity for the oxidative dehydrogenation (ODH) of propane to propylene. Cothermolyses of 4 and Bi[OSi(O{sup t}Bu){sub 3}]{sub 3} resulted in formation of Bi/Mo/P/Si/O materials with improved performance for the ODH of propane.

  2. OT1_cjoblin_1: Physics of gas evaporation at PDR edges

    NASA Astrophysics Data System (ADS)

    Joblin, C.

    2010-07-01

    Far-ultraviolet (FUV) photons in massive OB star-forming regions have a major impact on the structure, dynamics, chemistry and thermal balance of their associated molecular cloud. We propose to study the photoevaporation under FUV irradiation of dense filaments in prototype photodissociation regions (PDR) by mapping with the HIFI spectrometer the [CII] 158 micron line associated with the evaporating gas and high-J CO lines tracing the warm dense structures. The combination of the spectral range covered by Herschel and the very high spectral resolution of HIFI is unique to get insight into the process of mixing of cold molecular gas into warm atomic gas. This process governs the evolution of dense gas submitted to FUV photons in a wide variety of astronomical objects including protostellar and protoplanetary disks but is best studied in PDRs. We ask for 9.8 hours of observations in two PDRs, NGC7023 and the Horsehead nebula. NGC7023 is illuminated by a B2Ve star and hosts very diluted atomic gas and dense filaments. The Horsehead nebula is a PDR viewed nearly edge-on with a high gas density gradient at the edge that is illuminated by a O9.5V star and is immersed in an HII region. In these objects, there is evidence for dynamical processes that create a mixing layer between molecular and atomic gas, both from gas kinematics (first results with HIFI on the [CII] line) and chemistry. The first HIFI [CII] results clearly call for a larger spatial coverage of the region using OTF mapping mode with HIFI to obtain a more complete picture of the PDR morphology and dynamics. The 12^CO(8-7) and 13^CO(8-7) lines will be also targeted to trace the warm interfaces of the dense filaments/edges. The team gathers together specialists of the studied regions and of the Herschel instruments: HIFI (this proposal), SPIRE and PACS (complementary data). The team has strong expertise in the study of the physics and chemistry of PDRs, both in terms of data analysis and modelling using and developing the Meudon PDR code.

  3. OT2_dteyssie_2: Unveiling the water puzzle in cold PDRs: The Horsehead case

    NASA Astrophysics Data System (ADS)

    Teyssier, D.

    2011-09-01

    While Herschel observations of water vapour in warm regions (T_gas >> 100 K) driven by energetic processes (outfows, shocks, hot cores, etc.) have confirmed large abundances of water vapour (~10^-5), the inferred gas phase H2O abundance in dark clouds is very low, ~10^-10. A detailed balance between freeze-out, ice-mantle desorption and gas-phase chemistry is required to explain this orders-of-magnitude abundance difference. The Horsehead nebula is particularly well-suited to investigate grain surface chemistry in a UV irradiated environment. Its relatively low UV illumination (~60 in Draine units) implies low dust grain temperatures, from T_dust=30K in the PDR to T_dust=20K deeper inside the cloud. Therefore, the release of the grain mantle products into the gas phase, water vapour in particular, is dominated by UV-induced photo-desorption and not by thermal evaporation (as in other warm PDRs such as the Orion Bar). Besides, owing to its simple edge-on geometry, the Horsehead is very close to the prototypical kind of source needed to serve as model benchmark. A relatively low water vapour beam-averaged abundances (~5x10^-9) was derived from a single position of the o-H2O ground-state line towards the so-called "IR peak" of the Horsehead PDR. However a detailed comparison with sophisticated PDR models including gas and grain chemistry is not possible due to our complete ignorance about the true H2O spatial distribution as one moves from the UV-illuminated cloud surface to the inner shielded cloud. We propose here to map with HIFI the o-H2O 557 GHz line on a cut across the PDR front, and extract the spatial information required to constrain the role of water freeze-out and water ice photodesorption as a function of cloud depth.

  4. GIOTTO's antenna de-spin mechanism: Ots lubrication and thermal vacuum performance

    NASA Technical Reports Server (NTRS)

    Todd, M. J.; Parker, K.

    1987-01-01

    Except in the near Earth phase of GIOTTO's mission to Comet Halley, the HGA (high gain antenna) on board GIOTTO was the only designed means of up/down communications. The spacecraft spin stabilization required that the HGA be despun at the same rotational rate of nominally 15 rpm in order to keep the HGA pointing accurately to a Earth. A dual servomotor despin mechanism was designed and built by SEP of France for this purpose. The expected thermal environment suggested that dry lubrication was preferable to wet for the ball bearings but there existed no relevant data on the torque noise spectrum of candidate solid lubricants. Therefore ad hoc torque noise tests were run with two solid lubricants: ion plated lead film plus lead bronze cage (retainer) and a PTFE composite cage only. The lead lubrication showed the better spectrum up to the mission lifetime point so it was selected for continued test over some 20 times the Halley mission life, with periodic torque spectrum monitoring. The spectrum remained well within the pointing error budget over the 100 million revolutions covered.

  5. A Guide to Reasonable Accommodation for OT Practitioners with Disabilities: Fieldwork to Employment.

    ERIC Educational Resources Information Center

    Wells, Shirley A., Comp.; Hanebrink, Sandy, Comp.

    This handbook provides information to occupational therapy clinicians and students with disabilities, fieldwork educators, and employers on issues of accommodation, disclosure, rights, responsibilities, and the Americans with Disabilities Act (ADA). It begins with a discussion of the disability rights movement, and employment and people with…

  6. 78 FR 54401 - Removal of Transferred OTS Regulations Regarding Post-Employment Activities of Senior Examiners

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-04

    ... of the 550 17th Street Building (located on F Street) on business days between 7 a.m. and 5 p.m... implemented section 6303(b) of the Intelligence Reform and Terrorism Prevention Act of 2004.\\4\\ This Act...

  7. Local and Global Magnetic Fields of Late-Type Dwarfs OT Ser and YZ CMi

    NASA Astrophysics Data System (ADS)

    Bychkov, V. D.; Bychkova, L. V.; Madej, J.; Panferov, A. A.

    2015-04-01

    Differential rotation is the primary energy source for generation of local magnetic fields in the atmospheres of late-type stars (Moss et al. 1995). Moreover, the colder a star, the greater the effect, which was confirmed by observations. For instance, Saar (1988) measured the surface magnetic fields of late-type stars using the integral method Robinson (1980) and drew attention to the fact that the average magnetic field in the spots reaches the values of 1500 G for the G-type dwarfs, 2500 G for the K dwarfs, and 3500 G for the M-dwarf stars. The fraction of the surface of a star covered by spots also increases towards the latest spectral types. These well-known observational facts were explained by theoretical models, such as the α2 mechanism, for instance (Moss et al. 1995). Late-type dwarfs exhibit periodic eruptions resulting from the field line reconnection of newly generated local magnetic fields. This feature is observed in G, K, and M dwarfs, constituting 95%of all the stars in our Galaxy. The most prominent are the field reconnections in the so-called flare stars, which are the M dwarfs. This is understandable, since the peak flux of M dwarfs is placed in the red and infrared regions of the spectrum, whereas a flash has the maximum emission in the violet spectral region. Analysis of long-term photometric observations revealed that, on the average, energy of flares was found constant over a long time period for each flare star. That is to say, this conclusion implies that the power of the local magnetic field generator remains constant at this stage of evolution of stars.

  8. Independent evaluation plan for radiac set AN/VDR-1() Operational Test IIA (OT IIA)

    SciTech Connect

    Not Available

    1980-02-01

    The AN/VDR-1() is being developed in response to a DA approved Qualitative Materiel Requirement (QMR) dated 3 March 1971. The radiac system must provide a means of conducting both dismounted and vehicular radiological surveys and for performing radiological monitoring of personnel and equipment. The system will replace both the IM-174/PD and IM-174A/PD radiacmeters and may replace the AN/PDR-27() radiac set. This system is not envisioned for use as an aerial survey meter, since the AN/ADR-6 is currently under development for that specific task. The system will be operated by the individual soldier. A driver should be able to operate it during vehicular radiological surveys. The system will be a TOE issue item to Army units. The equipment will not normally be pooled at higher echelons, except as maintenance floats. The basis of issue will be one system per platoon, company headquarters and subunit requiring a capability to detect low or high level contamination (e.g., medical section). The system will be operated in various climatic and weather conditions. The system will provide the commander with data concerning gamma dose rates in areas contaminated by fallout, neutron-induced gamma activity or radiological agents. This data will assist in the planning of tactical operations and medical monitoring of radiological casualties.

  9. OT1_dneufeld_2: The puzzle of water vapour in carbon-rich stars

    NASA Astrophysics Data System (ADS)

    Neufeld, D.

    2010-07-01

    Using the HIFI instrument, we will address the puzzling - but widespread - appearance of water vapour in carbon-rich stars. Following up on detections of water in ALL SIX carbon-rich AGB stars observed to date in a pilot study performed in the HIFISTARS Key Program, we will target additional water transitions in four stars already observed or expected to show the most luminous water emissions. The target stars are CIT6, IRAC 15194-5155, V Cygni, and S Cep, and the additional transitions are the 4(22)-3(31) and 3(12) - 2(21) transitions at 916 GHz and 1153 GHz. Combined with spectra already obtained for the low-lying water transitions, and interpreted in the context of water excitation models, the proposed observations will place strong constraints upon the location of the emitting water. We will therefore be able to distinguish between various hypotheses that have been proposed for the origin of the observed water: the vaporization of orbiting comets or dwarf planets; catalytic formation on dust grains; or chemical processes initiated by the photodissociation of CO. In addition, we will carry out deep integrations to observe the lowest 1(11) - 0(00) transition of para-water at 1113 GHz in two carbon-rich AGB stars: IRAS+40540 and V Hya; here, ortho-water has been securely detected but existing observations of the 1113 GHz para-water line yield weak detections that lack the signal-to-noise ratio needed to constrain the ortho-to-para ratio.

  10. OT2_fwyrowsk_3: A Water survey of massive star forming clumps in the inner Galaxy

    NASA Astrophysics Data System (ADS)

    Wyrowski, F.

    2011-09-01

    Water, as a dominant form of oxygen, the most abundant element in the universe after H and He, controls the chemistry of many other species. It is a unique diagnostic of warm gas and energetic processes taking place during star formation. We therefore propose to exploit the unique opportunity of Herschel to study water in large, statistically significant, flux limited samples of massive star forming regions detected in the recently completed ATLASGAL submm dust continuum survey of the inner Galactic plane. In the last years, our view of massive star forming regions has dramatically changed by Galactic plane surveys covering cm to IR wavelengths. These surveys enable us for the first time to study ALL evolutionary stages of massive star formation (MSF) in an unbiased way. Water, acting as a natural filter for warm, dense gas, allows to probe the chemical and physical conditions in all of these stages close to where the massive stars are forming or just have been formed. ATLASGAL observed submm dust continuum emission as best tracer of the earliest phases of MSF since it is directly probing the material from which the stars form. As a large unbiased survey it provide the statistical base to study the scarce and short-living protoclusters as the origin of the massive stars and the richest clusters in the Galaxy and supplies us with a legacy value sample of MSF regions for the water follow ups. Water is typically seen with strongly increased abundances in broad line wings, providing a new, sensitive probe of shocked outflowing gas. In addition, the envelope is probed in a combination of absorption and emission with a clear jump in abundance in the warm inner regions close to the forming massive stars. Only Herschel can provide a water survey of a large sample of ATLASGAL selected sources to study water through the evolution of massive star forming regions with a statistically significant sample size.

  11. OT2_ctibbs_1: Exploring the role of CII in current Spinning Dust Models

    NASA Astrophysics Data System (ADS)

    Tibbs, C.

    2011-09-01

    We propose HIFI observations of the CII fine structure line at 158 micron (1.9THz) in 22 pointings distributed across four Galactic anomalous emission regions (the Perseus cloud, LDN 1780, LDN 675 and LDN 1111). The currently favoured explanation for the observed anomalous microwave emission is that of electric dipole radiation from rapidly rotating small dust grains (PAHs and/or VSGs), commonly referred to as spinning dust. Although this hypothesis predicts that the source of the excess emission is due to dust, the small dust grains are sensitive to the ionisation state of the gas, and hence the spinning dust models have a dependancy on the abundance of the major gas ions. CII observations will enable us to investigate this dependancy, and combining these observations with the available mid- to far-IR observations will permit a complete analysis of the role of both the dust and gas in regions of anomalous emission. We request a total of 14.9 hrs of HIFI observing time.

  12. OT1_rpaladin_1: PACS and SPIRE observations of Galactic anomalous emission sources.

    NASA Astrophysics Data System (ADS)

    Paladini, R.

    2010-07-01

    Despite the increasing evidence that the anomalous emission is a new physical mechanism acting in the diffuse interstellar medium, the nature and distribution of this component remains elusive. The currently most favored models attribute the observed microwave excess to rotating very small dust grains (PAHs and VSGs). Nonetheless, the infrared properties of the sources which, to date, are known to exhibit this type of emission are very poorly known mostly due to the limited angular resolution and frequency coverage of DIRBE and IRAS data. We propose HERSCHEL PACS and SPIRE mapping of three Galactic anomalous emission sources (LDN 1780, LDN 675 and LDN 1111). This data, when combined with ancillary NIR and mid-IR data of comparable angular resolution (mainly from Spitzer), and coupled with available dust models, will allow to set tight constraints on the radiation field in the emitting sources as well as in their immediate surroundings. Such constraints, in turn, will allow to estimate the abundances of PAHs, VSGs and BGs, hence to shed light on the potential link between these dust populations and the observed microwave excess.

  13. OT2_cmccoey_3: Evolution of water emission in intermediate mass YSOs

    NASA Astrophysics Data System (ADS)

    McCoey, C.

    2011-09-01

    Intermediate mass YSOs are studied as part of the WISH KP. We find our source sample is too limited to identify properties associated with stellar evolution. With this proposal we ask for time to observe two more intermediate mass YSOs with HIFI and PACS. When looking at line profiles among or source set a confused picture appears. NGC 7129 FIRS 2 and LDN 1641 are both Class 0 YSOs but have very different line profiles. Similarly, the Vela sources are both Class I sources and even have almost the same bolometric luminosity but display very different spectra. It is noteworthy that line profiles of the Class 0 NGC 7129 FIRS 2 are very similar to the line profiles of the Class I Vela IRS 17. Furthermore, the most evolved source, AFGL 490, is most similar, albeit brighter, to one of the least evolved sources, LDN 1641. We require more observations in order to assess if there is any relation in line profile with evolutionary state. We ask for time to observe the Class 0 intermediate YSO, Cep E-mm, and the Class I/II sources, LkHa 224.

  14. 78 FR 54403 - Removal of Transferred OTS Regulations Regarding Recordkeeping and Confirmation Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-04

    ... State savings associations, for which the FDIC is the appropriate Federal banking agency. The FDIC also... the appropriate federal banking agency will be found at part 344. DATES: Comments must be received on... savings associations, and the Board of Governors of the Federal Reserve System (``FRB''), as to...

  15. Swift UVOT observations of the SMC nova MASTER OT J010603.18-744715.8

    NASA Astrophysics Data System (ADS)

    Kuin, N. P. M.; Page, K. L.; Williams, S. C.; Darnley, M. J.; Shore, S. N.; Walter, F. M.

    2016-10-01

    Since its discovery at magnitude 10.9 on 2016-10-14.19341 UT (Shumkov et al, ATEL #9621) and spectroscopic confirmation (Williams and Darnley, ATEL #9628) pre-discovery data from the OGLE team (Mroz and Udalski, ATEL #9622, and MASTER (Lipunov et al., ATEL #9631) revealed the progenitor magnitude and pushed back the appearance of the nova to magnitude 8.5 on 2016, Oct 9.808.

  16. I Have Students with Physical and Motor Problems: How Can an APE, OT, or PT Help?

    ERIC Educational Resources Information Center

    Silliman-French, Lisa; Candler, Catherine; French, Ron; Hamilton, Merry Lynne

    2007-01-01

    Majority of students with mental and/or motor impairments are frequently placed in general physical education classes. However, these students often are unable to attain the expected outcomes in these general settings. Based on the experience of the authors, general physical educators often attempt to modify their goals, objectives, teaching…

  17. OT2_emurph01_3: The Herschel EDGE-on galaxy Survey (HEDGES)

    NASA Astrophysics Data System (ADS)

    Murphy, E.

    2011-09-01

    We propose deep PACS and SPIRE imaging for all (6) normal, edge-on (i >85 deg) galaxies with d<10Mpc as part of the Herschel EDGEon galaxy Survey (HEDGES). These data will allow us, for the first time, to characterize variations in the far-infrared (FIR) spectral energy distribution (SED) among and within halos for an energetically diverse sample of nearby, edge-on galaxies as a function of height above the disk. The proper sampling of the halo dust SEDs (i.e., 6 bands between 70500um), will allow us to take inventory of the dust content of halos and illuminate the signatures of dust processing imprinted in the dust SED and its variations. These observations will provide a way to address a number of outstanding questions related to the processes governing the interchange of disk/halo material such as: (1) How does halo dust content relate to disk star formation activity? (2) What are the physical characteristics of halo dust (i.e., temperature(s), mass, emissivity, PAH mass fraction, and what does their variation with height from the plane tell us about grain modification by the energetic processes responsible for disk-halo cycling? (3) What can dust and radio continuum halos tell us about transport effects that are important for understanding the FIR-Radio correlation? (4) How does the distribution of halo dust compare to that of other gas tracers, and hence what can we learn about how such dust is associated with various gas phases? The HEDGES sample galaxies have a treasury of ancillary data (e.g., radio, Spitzer, optical, gas), and the addition of these deep Herschel data will make HEDGES the definitive sample to study multi-phase disk/halo feedback processes arising from the accretion, expulsion, and/or cycling of material between disks and halos into the foreseeable future.

  18. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I...) The total votes cast in favor of and against each matter. (5) The percentage of votes necessary to approve each matter. (6) An opinion of counsel that you conducted the members' meeting in compliance...

  19. 12 CFR 563b.240 - What must I submit to OTS after the members' meeting?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... TREASURY CONVERSIONS FROM MUTUAL TO STOCK FORM Standard Conversions Vote by Members § 563b.240 What must I...) The total votes cast in favor of and against each matter. (5) The percentage of votes necessary to approve each matter. (6) An opinion of counsel that you conducted the members' meeting in compliance...

  20. Bulgarski ezik za dobrovoltsi ot Korpusa na mira (Bulgarian Language for Peace Corps Volunteers. Bulgaria).

    ERIC Educational Resources Information Center

    Dimitrova, Aneta; Tomova, Christina; Tasseva, Mila

    This workbook and class guide for basic-to-intermediate Bulgarian provides a communicative orientation to the language. With its emphasis on social situations and everyday language, the book includes vocabulary, dialogs, discussion topics, and cultural-historical background information about Bulgaria. (CNP)

  1. Bulgarski ezik za dobrovoltsi ot Korpusa na mira (Bulgarian Language for Peace Corps Volunteers. Bulgaria).

    ERIC Educational Resources Information Center

    Dimitrova, Aneta; Tomova, Christina; Tasseva, Mila

    Designed for continued language learning beyond the intermediate level, this workbook provides practice and sets of exercises for improving speaking and listening skills in Bulgarian through class participation and discussion, and reading and writing. The book includes grammar explanations, news reports, brief biographies of prominent Bulgarians,…

  2. WHY DO THE ACUTE BEHAVIORAL EFFECTS OT TOLUENE IN RATS DEPEND ON THE ROUTE OF EXPOSURE?

    EPA Science Inventory

    Despite evidence suggesting that the acute effects of organic solvents are related to their concentration in the brain, we have observed route-dependent differences in the acute behavioral effects of toluene. Whereas inhaled toluene disrupts the performance of rats on a visual si...

  3. OT1_jberna01_1: Characterising the Environment of Fullerene Formation

    NASA Astrophysics Data System (ADS)

    Bernard-Salas, J.

    2010-07-01

    The bulk of the dust that is injected into the interstellar medium is formed via a complex chemistry in AGB circumstellar environments, which transforms the atomic gas into molecules and dust. To date, more than 60 individual molecular species of both inorganic and organic nature and a handful of dust minerals have been identified in these outflows. These environments are also thought to be the birthplace for large aromatic species such as polycyclic aromatic hydrocarbons and fullerenes. Because of their remarkable stability, fullerenes have been predicted to survive the harsh conditions of the insterstellar medium. However, targeted searches for the presence of fullerenes in various astrophysical environments were unsuccessful or not conclusive, until now. The Spitzer mid-IR spectrum of Tc1, a young planetary nebula with a low excitation central star and a high C/O abundance ratio, shows clear traces of C60 and C70. This indicates that when conditions are favorable, fullerenes are formed in large quantities. The mid-IR spectra allow us to quantify the abundance of carbon in the fullerenes, estimate their temperature, and detect them in the solid state in circumstellar environments, but to date we have little information on the circumstellar dust or the composition of the gas. We propose to use the far-IR spectrum of Tc1 using the Herschel PACS spectrometer to study the energy balance and characterize the conditions in the circumstellar regions where fullerenes are formed.

  4. Increased radiosensitivity of a subpopulation ot T-lymphocyte progenitors from patients with Fanconi's anemia

    SciTech Connect

    Knox, S.J.; Wilson, F.D.; Greenberg, B.R.; Shifrine, M.; Rosenblatt, L.S.; Reeves, J.D.; Misra, H.

    1981-06-01

    In vitro radiation survival of peripheral blood T lymphocytes was studied in 15 clinically normal adults and 4 patients with Fanconi's anemia. Tritiated thymidine incorporation in a whole blood lymphocyte stimulation test (LST) and a newly developed whole blood T-lymphocyte colony assay were used to measure lymphocyte blastogenesis and colony formation in response to phytohemagglutinin (PHA) or concanavalin-A (Con-A) stimulation. Lymphocyte colony formation was found to be consistently more sensitive than the LST for detection of low-level radiation effects using both normal cells and lymphocytes from Fanconi's anemia patients. Lymphocytes from patients with Fanconi's anemia were significantly more sensitive to in vitro x-irradiation than lymphocytes from clinically normal individuals as measured by their ability to divide when stimulated by PHA in the LST (patients, D37 . 198 R; normals, D37 . 309 R, p . 0.057) and colony formation assay (patients, D37 . 53 R; normals, D37 . 109 R, p . 0.016). No significant difference in the radiosensitivity of the Con-A response was observed between the two groups. The PHA-responsive T-lymphocyte subpopulation in Fanconi's anemia patients appears to be intrinsically defective. The nature of this defect, significance in the disease process, and relevancy of these findings to the establishment of radiation protection standards are discussed.

  5. OT1_jgreen02_2: FOOSH: FU Orionis Objects Surveyed with Herschel

    NASA Astrophysics Data System (ADS)

    Green, J.

    2010-07-01

    We propose to utilize the unprecedented spatial resolution and sensitivity of Herschel at far-infrared and submm wavelengths to observe nearly all known FU Orionis objects, the dramatic result of burst accretion events in protostellar disks. The known FUors represent a vital window into a key process of star formation rather than a rare and peculiar event in the lives of a few stars. In addition, FUors provide a natural laboratory that probes the effect on enhanced heating on disk composition and structure. Our objectives are to (1) Study the structure of known envelopes and constrain the amount of remnant envelope material around the remainder; (2) characterize the physical and chemical properties of the disks and envelope, the parameters that set the initial conditions for planet formation in T Tauri disks; (3) observe solid-state, atomic, and molecular spectral features toward FUors in order to determine the effects of increased luminosity on mineralogy, disk chemistry, and envelope material. In order to do this we will use all three instruments onboard Herschel, providing a comprehensive survey of FUors.

  6. 78 FR 76721 - Removal of Transferred OTS Regulations Regarding Recordkeeping and Confirmation Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... security holders. (b) Cash management sweep account means a prearranged, automatic transfer of funds above.... 344.5. (d) Cash management sweep accounts. An FDIC-supervised institution effecting a securities transaction for a cash management sweep account shall give or send its customer a written statement, in...

  7. Definitize and Prepare Off-The-Shelf (OTS) Electronic Test Equipment (ETE) Specifications

    DTIC Science & Technology

    1978-07-01

    30V P/P ACROSS 4000 OHM LOAD 35V P/P 5V P/P ACRCSS 50 OHM LOAD 0 -15V ACROSS 50 OHM LOAD 50V P/P 50V P/P...LOAD 15V P/P 0 TO 100V ACROSS 600 OHM L04D 30V P/P 0 TO 7V P/P ACROSS 75 OHM LOAD 50V AC’DSS 0 TO...7V P/P ACROSS 75 OHM LOAD 50V ACRDS? 30V P/P ACROSS 4000 OHM LOAD 3 5V P/P 5V P/P ACRCSS 50 OHM LOAD 0 -15V

  8. Ursa Major: ot losya do medvedya %t Ursa Major: from elk to bear

    NASA Astrophysics Data System (ADS)

    Lushnikova, A. V.

    In the article material from various cultural and linguistic sources (Indo-European - Slavic, Indo-Iranian; Uralo-Altaic, Tungus, Ancient Egyptian, Babylonian) is used in order to trace up the chronology of the designation of Ursa Majopr, a constellation which has been playing an important role for people different regions since ancient times. It was used for observing of the visible yearly motion of the Sun, for working out seasonal changes; being a circumpolar and non-hiding behind the horizon it has been perceived as a symbol of immortality, its peculiar positional change during a year lay down in plot of the Uralo-Siberian myths about a cosmic hunt for the Elk, myths about deluge. Data from Uralo-Siberian mythology are analyzed. Designations of Ursa Major in the form of a horned hoofed animal such as elk, deer, cow (Uralo-Altaic, Tungus, Slavic, Indo-Iranian languages; Ancient Greece, Crete, Ancient Egypt) and connected with it (or derived from it) denominations and images of "enclosed space" - "vehicle for travelling and carrying goods" (a wagon, a boat) - "instrument for hunting-fishing, a ritual thing" - "household construction" are taken in consideration. The conclusion is made that the transition of the Ursa Major designation from elk to bear could follow the general tendency to shift from so-called matriarchy to patriarchy, to substitute female deities with male ones, which was reflected "in the rise" of the predatory animal cults (not earlier than II mil. B.C.). To prove this, lexical examples of resemblance and coincidences in designation of homed hoofed (elk, deer) and predatory (bear, wolf) animals should be analyzed. Such a goal-directed investigation of the chronology of Ursa Major designations has never been carried out.

  9. OT1_philybla_1: The chemistry of nitrogen in dark clouds

    NASA Astrophysics Data System (ADS)

    Hily-Blant, P.

    2010-07-01

    Nitrogen is the fifth most abundant element in the local Universe. It is essential component of molecules associated to Earth-type life. Yet, the reservoir of nitrogen in the dense ISM, where stars, and ultimately planets, form, is not known. This is for good reasons. The main reservoir of gas-phase nitrogen are expected to be N or N2, and it is likely that most of the nitrogen be indeed frozen-out on dust grains in the form of ammonia ices. However, N and N2 are not observable in the shielded environments characterizing the embryos of star-forming regions. Hence, all what is know about nitrogen must rely on indirect observations of N-bearing molecules, the lightest (and thus among the easiest to form) of which are hardly detectable from the ground. To date, and despite longstanding efforts, our comprehension of the chemistry of nitrogen remains elusive. The HSO/HIFI instrument is opening new avenues in this respect, allowing astronomers to readily detect nitrogen hydrides and several key species of the nitrogen chemistry. This is the aim of this proposal. We propose the observation of key species that are observable only with Herschel. Those include NH, NH2 and NH3 (in their ortho and para forms), as well as their deuterated isotopologues, and other pivotal species like CH and CH2. This corpus of observations in order will allow to assess our understanding of the chemistry of nitrogen in dark cloud conditions, and answer the fundamental and open question, whether dust processes are necessary catalysts in this chemistry.

  10. Experimental Validation of OT Solutions to the Comprehension-Production Dilemma

    ERIC Educational Resources Information Center

    Gierut, Judith A.

    2006-01-01

    The relationship between perception and production remains an unresolved issue within the study of phonological acquisition. Recent developments in optimality theory offer potentially new solutions to this long-standing problem; but thus far, the proposals that have been advanced are in the absence of actual perception-production data from a given…

  11. Position Location Reporting System (PLRS) OT (Operational Test) II Human Factors and Training Evaluation

    DTIC Science & Technology

    1984-07-01

    W (U4 oC) .- 4 0 { 0.. L. CC E I. isla ad ontolSttin 0 0 0U 0 0 4) J :3000 C~ > . 0 U LLO 00 0 5. V V.00 ( 02 0( X 1. Display and Control Station 5 0...0 0 0 24 4. Install/replace User Readout ( URO ) (applies only to manpack, surface vehicle, and auxiliary ground unit operators). 16 17 5 0 0 4 42...Replace manpack unit support 0-o equipment, such as sling, field > > . pack, pack frame or URO holder 0 W L q . 0 (applies only to manpack unit C

  12. OT1_shaileyd_1: Testing the XDR/High-J CO Paradigm in Nearby Galaxies

    NASA Astrophysics Data System (ADS)

    Hailey-Dunsheath, S.

    2010-07-01

    One of the most exciting first results from SPIRE and PACS spectroscopy is the detection of high-J CO emission in galaxies. Such emission has long been proposed as a tracer of X-ray dominated regions (XDRs) produced by AGN, and as a powerful diagnostic tool for future millimeter-wave study of AGN at high redshift. The shortest wavelength submillimeter CO lines detected by SPIRE-FTS in the X-ray luminous ULIRG Mrk 231 are interpreted as arising from an extended XDR, providing strong observational support for the XDR/high-J CO connection. However, our group (SHINING; PI E. Sturm) has used PACS to detect even higher-J far-IR CO emission in a few nearby galaxies, including 2 starburst galaxies with little evidence of a luminous AGN. Can high-J CO emission also be produced in gas heated by the UV radiation or mechanical output of a starburst? To address this question we propose to measure a set of far-IR CO lines in 4 nearby AGN and 4 starburst galaxies, as well as in 2 merging systems with large masses of shock-heated molecular gas. Does the high-J CO line SED reflect the different excitation mechanisms in these template objects? We additionally propose to use OH line observations to estimate the abundance of this molecule, which is a sensitive tracer of X-ray-driven chemistry. The OH line profiles will also be used to search for evidence of molecular outflows, which may drive shock heating. Each of these galaxies will have SHINING GT observations of the set of far-IR fine-structure lines, which includes important tracers of UV-, X-ray-, and shock-heated gas. The AGN subsample is restricted to the most nearby systems, where the high spatial resolution of PACS is sufficient to separate the nuclear AGN-heated gas from the circumnuclear star-forming regions. The PACS CO data obtained here will provide the first well-sampled far-IR extragalactic CO line SEDs, and will be an essential reference for future high redshift studies.

  13. OT2_mcordine_2: Revealing the nature of the remarkable object IRAS 19312+1950

    NASA Astrophysics Data System (ADS)

    Cordiner, M.

    2011-09-01

    The IRAS source 19312+1950 is a peculiar bipolar nebula that has eluded firm characterisation since its discovery. It exhibits maser and outflow properties similar to a massive O-rich AGB star, but shows molecular species such as CH3OH and HC3N that are more typically found in molecular clouds or YSOs. The source is surrounded by remarkable NIR nebulosity and has an unusual SED. The puzzle over the true nature of this object is confounded by our Spitzer IR spectrum that shows amorphous silicates and CO2 ice, but also emission from what may be crystalline silicates. In order to understand the physical properties of the gas and dust in the vicinity of IRAS 19312+1950, we propose to perform Herschel observations of emission from CO and H2O across a broad wavelength range from sub-mm to IR. Observations of transitions from a variety of energy levels will allow us to probe different temperature and density regimes within the source, from which we will construct a picture of its physical structure through radiative transfer/excitation modelling. HIFI observations will allow us to separate the broad and narrow molecular line components and PACS IFU mapping will provide crucial spatial information on the physical structure of the source. We also propose to perform a PACS SED scan to better characterise the source SED, and measure diagnostic spectral features of the dust and gas such as the forsterite 69 micron band, the OI 63 micron line and the N II 122 and 205 micron lines. The combination of these unique observations will help solve the puzzle of the nature of this peculiar object.

  14. ICT and OTs: a model of information and communication technology acceptance and utilisation by occupational therapists.

    PubMed

    Schaper, Louise K; Pervan, Graham P

    2007-06-01

    There is evidence to suggest that health professionals are reluctant to accept and utilise information and communication technologies (ICT) and concern is growing within health informatics research that this is contributing to the lag in adoption and utilisation of ICT across the health sector. Technology acceptance research within the field of information systems has been limited in its application to health and there is a concurrent need to develop and gain empirical support for models of technology acceptance within health and to examine acceptance and utilisation issues amongst health professionals to improve the success of information system implementation in this arena. This paper outlines a project that examines ICT acceptance and utilisation by Australian occupational therapists. It describes the theoretical basis behind the development of a research model and the methodology being employed to empirically validate the model using substantial quantitative, qualitative and longitudinal data. Preliminary results from Phase II of the study are presented. The theoretical significance of this work is that it uses a thoroughly constructed research model, with potentially the largest sample size ever tested, to extend technology acceptance research into the health sector.

  15. The Transition from Spacecraft Development Ot Flight Operation: Human Factor Considerations

    NASA Technical Reports Server (NTRS)

    Basilio, Ralph R.

    2000-01-01

    In the field of aeronautics and astronautics, a paradigm shift has been witnessed by those in academia, research and development, and private industry. Long development life cycles and the budgets to support such programs and projects has given way to aggressive task schedules and leaner resources to draw from all the while challenging assigned individuals to create and produce improved products of processes. however, this "faster, better, cheaper" concept cannot merely be applied to the design, development, and test of complex systems such as earth-orbiting of interplanetary robotic spacecraft. Full advantage is not possible without due consideration and application to mission operations planning and flight operations, Equally as important as the flight system, the mission operations system consisting of qualified personnel, ground hardware and software tools, and verified and validated operational processes, should also be regarded as a complex system requiring personnel to draw upon formal education, training, related experiences, and heuristic reasoning in engineering an effective and efficient system. Unquestionably, qualified personnel are the most important elements of a mission operations system. This paper examines the experiences of the Deep Space I Project, the first in a series of new technology in-flight validation missions sponsored by the United States National Aeronautics and Space Administration (NASA), specifically, in developing a subsystems analysis and technology validation team comprised of former spacecraft development personnel. Human factor considerations are investigated from initial concept/vision formulation; through operational process development; personnel test and training; to initial uplink product development and test support. Emphasis has been placed on challenges and applied or recommended solutions, so as to provide opportunities for future programs and projects to address and disposition potential issues and concerns as early as possible to reap the benefits associated with learning from other's past experiences.

  16. OT1_hmaness_1: Planets, Debris Disks, and the Lambda Bootis Stars

    NASA Astrophysics Data System (ADS)

    Maness, H.

    2010-07-01

    We propose to explore the link between lambda Bootis stars, debris disks, and planetesimal formation and evolution. The lambda Boo stars are a rare type of peculiar A star (2%), which are Population 1 and metal poor. Planet bearing systems and debris disk stars appear unusually well represented in the lambda Boo class: for example, beta Pic, Vega, and HR 8799 are all lambda Boo candidates. A small sample of 14 lambda Boo stars observed by Spitzer suggests an occurrence of infrared excess approaching 100%. Only two lambda Boo stars are included in the DEBRIS/DUNES Herschel key program debris disk surveys. We will use PACS/Herschel to make sensitive, high-resolution maps of 27 new lambda Boo stars. Like DEBRIS/DUNES, we will reach the stellar photosphere for all targets, enabling a measurement of the true rate of excess infrared emission among lambda Boo stars compared to normal A stars. The depletion pattern of heavy elements in the atmospheres of lambda Boo stars suggests they may have accreted gas from which dust grains have condensed and been removed: this gas may be circumstellar gas that has formed planetesimals or dusty interstellar gas. While the circumstellar disk scenario predicts sizes of a few hundred AU, the cloud accretion scenario predicts 1000-2000 AU bow structures oriented in the direction of the relative motion of the cloud and star. With target distances of < 140 pc, these bow structures are expected to be resolved for all targets. These will be the first mid-infrared observations of lambda Boo stars outside of the low density Local Bubble: if interstellar medium interactions dominate the lambda Boo phenomenon then systematic variations in excess strength and morphology may occur with distance.

  17. The Temperature Dependence of Ice Viscosity (Vyazkost Lda v Zavisimosti ot Temperatury),

    DTIC Science & Technology

    The role and significance of crystalline structure in the study of the plastic properties of ice has been demonstrated. An anomalous change in the...rate of plastic deformation of specimens of sufficiently regular crystalline structure occurs when they are subjected to sharp change in temperature

  18. OTS: a program for converting Noldus Observer data files to SDIS files.

    PubMed

    Bakeman, R; Quera, V

    2000-02-01

    A program for converting Noldus Observer data files (ODF) to sequential data interchange standard (SDIS) files is described. Observer users who convert their data files can then take advantage of various flexible and powerful data modification and computational procedures available in the Generalized Sequential Querier, a program that assumes SDIS-formatted files.

  19. Visible-light driven water splitting over BiFeO₃ photoanodes grown via the LPCVD reaction of [Bi(OtBu)₃] and [Fe(OtBu)₃]₂ and enhanced with a surface nickel oxygen evolution catalyst.

    PubMed

    Moniz, Savio J A; Blackman, Christopher S; Southern, Paul; Weaver, Paul M; Tang, Junwang; Carmalt, Claire J

    2015-10-21

    Phase-pure BiFeO3 films were grown directly via dual-source low-pressure CVD (LPCVD) from the ligand-matched precursors [Bi(O(t)Bu)3] and [Fe(O(t)Bu)3]2, without the requirement for oxidising gas or post deposition annealing. Photocatalytic testing for water oxidation revealed extremely high activity for PEC water splitting and photocatalytic water oxidation under visible light irradiation (λ > 420 nm) with a benchmark IPCE for BiFeO3 of 23% at 400 nm. The high activity is ascribed to the ultrafine morphology achieved via the LPCVD process. The performance was enhanced by over four times when the BiFeO3 photoanode is coupled to a Ni-B surface OEC.

  20. Software Supportability Risk Assessment in OT&E (Operational Test and Evaluation): Historical Baselines for Risk Profiles. Volume 1.

    DTIC Science & Technology

    1985-10-07

    ORS OC-ALC 8-52 BNST OC-ALC B-52 FTSS OC-ALC 8-52 MC-1 EXEC 00-ALC B-52 MC-2 EXEC OC-ALC E- 3 INS OC-ALC E-3A OMEGA OC-ALC E-3A SMCP OC-ALC E-3A SRCP OC...293 SOFTIAR SWORTRILITY RISK ASSESSMENT IN OTAin 3 (OPERRTIONAL TEST RIM EVA.. (U) ION CORP RLIIUIERWE ENY TI.f CT9 W-5050T-O- WCMLSSIFIED F2901-U...lb. 1*ESTfl.CTIVI; MA04KING.- N/A None .SECURITY~ CLASSIFICATION AbUTNOAITY 3 . OISRIUUTION/AVAILAUILITY UPF MIORT N/A Unlimited -- O CLASSI P SCAT

  1. OT1_jsimon01_1: A Population of Dusty B Stars in the SMC: The First Extragalactic Debris Disks?

    NASA Astrophysics Data System (ADS)

    Simon, J.

    2010-07-01

    Using data from the Spitzer Survey of the SMC, we have discovered a population of 120 main sequence B stars with large 24 micron excesses. Optical spectroscopy and the IRAC SEDs demonstrate that they are not ordinary YSOs or Be stars. We suggest instead that these objects may be debris disks around massive main sequence stars. Confirmation of this hypothesis would provide one of the only ways to study the process of planet formation in a low-metallicity external galaxy. We have measured the mid-IR SED of the dust emission with IRS spectroscopy and determined that both cold and warm dust is present. We now propose PACS photometry at 70 microns to unambiguously separate the dust into its warm (and therefore circumstellar) and cold (possibly interstellar) components. These data will enable us to determine how much of the dust is warm and better constrain the temperature distribution; any targets with substantial amounts of warm dust are almost certainly debris disks. If the B stars do indeed host debris disks, they provide perhaps the only plausible method for constraining planet formation in an external galaxy for the foreseeable future.

  2. The first N-terminal unprotected (Gly-Aib)n peptide: H-Gly-Aib-Gly-Aib-OtBu.

    PubMed

    Gessmann, Renate; Brückner, Hans; Petratos, Kyriacos

    2015-12-01

    Glycine (Gly) is incorporated in roughly half of all known peptaibiotic (nonribosomally biosynthesized antibiotic peptides of fungal origin) sequences and is the residue with the greatest conformational flexibility. The conformational space of Aib (α-aminoisobutyric acid) is severely restricted by the second methyl group attached to the Cα atom. Most of the crystal structures containing Aib are N-terminal protected. Deprotection of the N- or C-terminus of peptides may alter the hydrogen-bonding scheme and/or the structure and may facilitate crystallization. The structure reported here for glycyl-α-aminoisobutyrylglycyl-α-aminoisobutyric acid tert-butyl ester, C16H30N4O5, describes the first N-terminal-unprotected (Gly-Aib)n peptide. The achiral peptide could form an intramolecular hydrogen bond between the C=O group of Gly1 and the N-H group of Aib4. This hydrogen bond is found in all tetrapeptides and N-terminal-protected tripeptides containing Aib, apart from one exception. In the present work, this hydrogen bond is not observed (N...O = 5.88 Å). Instead, every molecule is hydrogen bonded to six other symmetry-related molecules with a total of eight hydrogen bonds per molecule. The backbone conformation starts in the right-handed helical region (and the left-handed helical region for the inverted molecule) and reverses the screw sense in the last two residues.

  3. XM587E2/XM724 Electronic Time Fuzes: Developmental Test/Operational Test (DT/OT) II Test Phase

    DTIC Science & Technology

    1979-11-01

    0. 322 + 0. 005 counterbore from the opposite side of the part. The now dimension to be 0. 173 - 0. 010 - to make the part more pro- ducible and aid...InspeCtion Operatio 0 3058 and 0555. 0010116 ML as~elt Slo Dfat viol Amin& (Low Limit) .04 $06 0 mIKO Armia (High Limit) .40 aO6 0 Rao) Wie-armngl .04

  4. Evidence of Cholesterol Accumulated in High Curvature Regions: Implication ot the Curvature Elastic Energy for Lipid Mixtures

    SciTech Connect

    Wang,W.; Yang, L.; Huang, H.

    2007-01-01

    Recent experiments suggested that cholesterol and other lipid components of high negative spontaneous curvature facilitate membrane fusion. This is taken as evidence supporting the stalk-pore model of membrane fusion in which the lipid bilayers go through intermediate structures of high curvature. How do the high-curvature lipid components lower the free energy of the curved structure? Do the high-curvature lipid components modify the average spontaneous curvature of the relevant monolayer, thereby facilitate its bending, or do the lipid components redistribute in the curved structure so as to lower the free energy? This question is fundamental to the curvature elastic energy for lipid mixtures. Here we investigate the lipid distribution in a monolayer of a binary lipid mixture before and after bending, or more precisely in the lamellar, hexagonal, and distorted hexagonal phases. The lipid mixture is composed of 2:1 ratio of brominated di18:0PC and cholesterol. Using a newly developed procedure for the multiwavelength anomalous diffraction method, we are able to isolate the bromine distribution and reconstruct the electron density distribution of the lipid mixture in the three phases. We found that the lipid distribution is homogenous and uniform in the lamellar and hexagonal phases. But in the distorted hexagonal phase, the lipid monolayer has nonuniform curvature, and cholesterol almost entirely concentrates in the high curvature region. This finding demonstrates that the association energies between lipid molecules vary with the curvature of membrane. Thus, lipid components in a mixture may redistribute under conditions of nonuniform curvature, such as in the stalk structure. In such cases, the spontaneous curvature depends on the local lipid composition and the free energy minimum is determined by lipid distribution as well as curvature.

  5. Software Supportability Risk Assessment in OT&E (Operational Test and Evaluation): Measures for a Risk Assessment Model

    DTIC Science & Technology

    1984-09-28

    being vulneraole to some degree to a particular outcome of an activity, if that outcome occurs. Extrapolation/Project ion (ROWE...and valida- tion: (1) Independent. An organization/agency which is seoarate from the software cevelooment activity r’rcm a contractual and

  6. 75 FR 181 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... collection, unless the information collection displays a currently valid OMB control number. As part of the...: December 28, 2009. Ira L. Mills, Paperwork Clearance Officer, Office of Thrift Supervision. [FR Doc....

  7. OT2_eegami_5: Herschel Lensing Survey II: Completing the Herschel Legacy with the HST/MCT CLASH Sample

    NASA Astrophysics Data System (ADS)

    Egami, E.

    2011-09-01

    For deep imaging longward of 100 um, confusion noise sets the fundamental sensitivity limits achievable with Herschel, and these limits cannot be improved by integrating longer. To penetrate through this confusion limit and detect faint high-redshift galaxies, gravitational lensing by massive galaxy clusters offers a very powerful and yet cheap solution. For this reason, our team has been conducting a PACS/SPIRE imaging survey of 44 massive lensing clusters as one of the Herschel Key Programs, "The Herschel Lensing Survey" (PI: Egami, 292.3 hrs). Deep PACS/SPIRE imaging data of massive clusters are quite rich with a variety of information, which allows us to study not only the properties of gravitationally lensed high-redshift galaxies but also those of cluster member galaxies and the intracluster medium through the analysis of the Sunyaev-Zel'dovich effect. In January 2010, a massive HST program targeting powerful lensing clusters was accepted as one of the three multi-cycle treasury (MCT) programs. This program, ``the Cluster Lensing And Supernova survey with Hubble'' (CLASH), has an allocation of 524 orbits, and will obtain deep ACS and WFC3 images of 25 massive galaxy clusters using 16 broad-band filters from near-UV (2250 A) to near-IR (1.6 um). These extensive multi-filter imaging observations will produce high-precision photometric redshifts (sigma/(1+z)<0.02). On average, the program spends 20 orbits per cluster. Considering this enormous investment of HST time, the CLASH program will define the ultimate sample of massive galaxy clusters on which future studies will focus. Here, we propose to obtain deep PACS and SPIRE images for 10 CLASH clusters that still lack such data (the other 15 clusters already have a good Herschel coverage). To fully exploit the combination of the Herschel and HST data, the HLS and CLASH teams are submitting this proposal jointly with the participation of key scientists from both teams.

  8. Software Supportability Risk Assessment in OT&E (Operational Test and Evaluation): An Evaluation of Risk Methodologies.

    DTIC Science & Technology

    1984-08-31

    DISTRIBUTION: UNIMITED) ELC 0 AUUST 31, M94 Wd/A 8 4 496-TR wwvw WW CORPORATION 1801 RANDOLPH ROAD. S.E * ALSUQUERQUE. NEW MEXICO 87106 *(505) 848-5=0...Road, S.E., Albuquerque, New Mexico , 87106, to the Air Force Operational Test and Evaluation Center, Kirtland Air Force Base, New Mexico , 87117. This...metrics described in section 3.4 should be reported in a storyboard fashion. This technique would act as a summary of the ri.sk assessment process

  9. High-Amplitude, Rapid Photometric Variation of the New Polar Master OT J132104.0+560957.8

    DTIC Science & Technology

    2015-02-05

    COMMISSIONS 27 AND 42 OF THE IAU INFORMATION BULLETIN ON VARIABLE STARS Number 6129 Konkoly Observatory Budapest 5 February 2015 HU ISSN 0374 – 0676...polar can undergo a precipitous drop in optical brightness if the accretion region rotates behind the limb of the WD or is eclipsed by the donor star ...Schmidt-Cassegrain telescope on 2012 December 23. We used the APASS survey (Henden et al. 2012) to find the Johnson V magnitudes of our comparison stars so

  10. "[N]ot subject to our sense” : Margaret Cavendish's fusion of Renaissance science, magic and fairy lore.

    PubMed

    Walters, Lisa

    2010-01-01

    This article explores Margaret Cavendish's depictions of alchemy, witchcraft and fairy lore in her scientific treatise Philosophical Letters and in fictional texts from Natures Pictures and Poems and Fancies. Though Cavendish was a dedicated materialist, she appropriates theories of magic from early modern science and folklore into her materialist epistemology. As Cavendish draws upon a fusion of early modern conceptions of magic, she creates a radical theory of matter which not only challenges patriarchy and binary oppositions, but also explores the plurality and mystery that can exist within an infinitely complex material world.

  11. OT2_cceccare_5: Peering into the engine of a jet-driven bowshock : TMC1B1

    NASA Astrophysics Data System (ADS)

    Ceccarelli, C.

    2011-09-01

    Jet-driven bowshocks seem to be rather ubiquitous in star-forming regions. If the large-scale bow is commonly observed and its kinematics, the actual engine of the outflow, the Mach disk region where the gas is accelerated, has never been detected until now. Recently, we have found observational evidence of the long-searched engine of outflows in the giant molecular bowshock TMC1B1 together with the driving jet, in Taurus (d=140 pc). which allows Herschel to resolve the cooling region of the shock. We propose to use Herschel to investigate the dynamics and physical structure of TMC1B1, a potential benchmark for jet-driven bowshocks studies.

  12. Neuroanesthesia management of neurosurgery of brain stem tumor requiring neurophysiology monitoring in an iMRI OT setting

    PubMed Central

    Sabbagh, Abdulrahmam J.; Al-Yamany, Mahmoud; Bunyan, Reem F.; Takrouri, Mohamad S. M.; Radwan, Sabry Mohammed

    2009-01-01

    This report describes a rare case of ventrally exophytic pontine glioma describing operative and neuroanesthesia management. The combination of intraoperative neuromonitoring was used. It constituted: Brain stem evoked responses/potentials, Motor EP: recording from cranial nerve supplied muscle, and Sensory EP: Medial/tibial. Excision of the tumor was done with intra-operative magnatic resonance imaging (iMRI), which is considered a new modality. PMID:20532111

  13. OT1_bmatthew_4: Testing Planetary Dynamics and Evolutionary History in the HR 8799 Planet/Disc System

    NASA Astrophysics Data System (ADS)

    Matthews, B.

    2010-07-01

    We propose to map the debris disc associated with the multi-planet system HR 8799 in order to constrain the current dynamical state of the planetary system and refine models for dust production in the disc, thereby testing models for the origins of the three known giant planets. Herschel's sensitivity and resolution make it possible to image both the cold planetesimal disc (posited to lie between radii of 90-300 AU) as well as the fainter extended halo (300 - 1000 AU radius) at multiple wavelengths. Direct detection of the edges of the cold belt of dust and an independent measure of the system's inclination will provide critical constraints on models of the planetary orbits within the system, particularly for the outer-most planet for which mass and orbit information can be constrained by simultaneous fits to the planet and disc. The combination of three massive, coeval, and spectroscopically characterizable planets, together with the dust disc, makes this system a "Rosetta Stone" for planet formation studies. The disc is also important for differentiating between planet formation scenarios. Models predict variations in resonance structure for migration versus in situ formation, and multi-wavelength variations in observed structure within Herschel's wavelength range in the case of planetary migration. This proposal is at the very heart of Herschel's top science goal of understanding the mechanisms involved in the formation of stars and planetary bodies. The resolution, sensitivity and multi-wavelength imaging of Herschel are crucial to this program.

  14. OT1_aedge_4: A definitive Herschel study of the most powerful local radio galaxy - Cygnus A

    NASA Astrophysics Data System (ADS)

    Edge, A.

    2010-07-01

    Powerful radio galaxies have played a central role in our understanding of active galaxies over the past five decades. Cygnus-A, the brightest and most nearby FR-II source, has been a "Rosetta Stone" in this study. It appears to have the outward characteristics of a normal radio galaxy but it's proximity allows us to identify the more subtle properties of an obscured quasar. The energy provided by this very active galaxy is sufficient to launch the energetic jets we observe but the transport of the energy to the hotspots is poorly constrained and understood. The growing realisation that AGN directly affect their host galaxy through a direct feedback mechanism throughout the lifetime of the galaxy makes it especially important for the most nearby obscured quasar to be observed with the widest possible spectral coverage. We propose to use Herschel to reveal the heating of the central region and the energetics of the hotspots. We request observations of the atomic cooling lines [OI], [OIII] and [CII] that will allow us to compare the energetics of the ISM in Cygnus A with other local and more distant galaxies.

  15. Crystal structure of product-bound complex of UDP-N-acetyl-D-mannosamine dehydrogenase from Pyrococcus horikoshii OT3

    SciTech Connect

    Pampa, K.J.; Lokanath, N.K.; Girish, T.U.; Kunishima, N.; Rai, V.R.

    2014-10-24

    Highlights: • Determined the structure of UDP-D-ManNAcADH to a resolution of 1.55 Å. • First complex structure of PhUDP-D-ManNAcADH with UDP-D-ManMAcA. • The monomeric structure consists of three distinct domains. • Cys258 acting as catalytic nucleophilic and Lys204 acts as acid/base catalyst. • Oligomeric state plays an important role for the catalytic function. - Abstract: UDP-N-acetyl-D-mannosamine dehydrogenase (UDP-D-ManNAcDH) belongs to UDP-glucose/GDP-mannose dehydrogenase family and catalyzes Uridine-diphospho-N-acetyl-D-mannosamine (UDP-D-ManNAc) to Uridine-diphospho-N-acetyl-D-mannosaminuronic acid (UDP-D-ManNAcA) through twofold oxidation of NAD{sup +}. In order to reveal the structural features of the Pyrococcus horikoshii UDP-D-ManNAcADH, we have determined the crystal structure of the product-bound enzyme by X-ray diffraction to resolution of 1.55 Å. The protomer folds into three distinct domains; nucleotide binding domain (NBD), substrate binding domain (SBD) and oligomerization domain (OD, involved in the dimerization). The clear electron density of the UDP-D-ManNAcA is observed and the residues binding are identified for the first time. Crystal structures reveal a tight dimeric polymer chains with product-bound in all the structures. The catalytic residues Cys258 and Lys204 are conserved. The Cys258 acts as catalytic nucleophile and Lys204 as acid/base catalyst. The product is directly interacts with residues Arg211, Thr249, Arg244, Gly255, Arg289, Lys319 and Arg398. In addition, the structural parameters responsible for thermostability and oligomerization of the three dimensional structure are analyzed.

  16. Free Energy of the Three-Dimensional Spin-12 Quantum Heisenberg Model to O[T6

    NASA Astrophysics Data System (ADS)

    Chang, Chih-chun

    2001-11-01

    By applying the Friedberg-Lee-Ren's theorem (Ann. Phys. (N.Y.) 228, 52 (1993)) to the spin-12 three-dimensional isotropic quantum Heisenberg system, we obtain the low-temperature expansion of the free energy through a field theoretical calculation done in the equivalent lattice boson system. We reproduced Dyson's result and also extended it from T5 to T6. Nevertheless, because of the peculiar property of the spin operator being neither bosonic nor fermionic, the extension is not easy to obtain by other method.

  17. Learning in a Simulation-OT in Heart Surgery and the Challenges of the Scientification of Work

    ERIC Educational Resources Information Center

    Langemeyer, Ines

    2014-01-01

    Enhancing competency and collaboration has become a salient topic of the professional debate on medical safety issues. The advantages of simulation-based training scenarios for team communication, routines and critical work procedures especially in operation theatres have been vigorously discussed. However, the literature on simulation-based…

  18. Multilocus phylogenetic analysis of true morels (Morchella) reveals high levels of endemics in Turkey relative ot other regions of Europe

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The present study was conducted to better understand how the phylogenetic diversity of true morels (Morchella) in Turkey compares with species found in other regions of the world. The current research builds on our recently published survey of 10 Turkish provinces and another of the world in which D...

  19. OT1_rrubin_1: Herschel's Opportunity to Solve the Nebular Abundance Problem While Creating a Legacy Planetary Nebulae Dataset

    NASA Astrophysics Data System (ADS)

    Rubin, R.

    2010-07-01

    Abundance surveys of a large sample of Galactic planetary nebulae (PNe) have led to the discovery of a group of super-metal-rich nebulae whose spectra show prominent optical recombination lines (ORLs) from C, N, O, and Ne ions. The heavy element abundances derived from ORLs for several PNe are a factor >10 higher than those derived from the traditional method based on collisionally excited lines (CELs). This ratio is called the abundance discrepancy factor (adf). A promising proposition to explain the nebular abundance problem posits that these nebulae contain (at least) two distinct regions - one of "normal" electron temperature, Te (~10000 K) and chemical composition (~solar) and another of very low Te (< 1000) that is H-deficient, thus having high metal abundances relative to H. The latter component emits strong heavy element ORLs and IR fine-structure (FS) CELs but essentially no optical/UV CELs. Efforts to directly detect these inclusions in PNe have been unsuccessful to date. However, there is mounting circumstantial evidence for their existence, such as presented in our recent paper that modeled the high-adf PN NGC 6153 using a 3-D photoionization code. The models that included the low Te, H-deficient knots fit most observations far better than did those models without the clumps. With the launch of Herschel, there is finally the capability to perform a test we've been dreaming of. Measurements have shown that the adf varies with position in a PN and is highest close to the central star. The very low Te inclusions must be cooled via FS IR lines. We propose to use Herschel to map the FS IR lines in 5 bright PNe on the largest adf list, to find if these lines peak where the adf peaks. These spectra will also provide a feast for our other team expertise/interests: a legacy dataset of molecular lines to explore PDRs, how the central star interacts with the AGB ejecta and shapes the PN, how the shocks are produced, what comprises the chemistry of the molecular ejecta, and how do PNe evolve.

  20. OT1_dweedman_1: Comparing [CII] 158 micron Luminosities to Spectral Properties of Luminous Starburst Galaxies and AGN

    NASA Astrophysics Data System (ADS)

    Weedman, D.

    2010-07-01

    Herschel PACS spectroscopy of the [CII] emission line at 158 microns is proposed for a carefully selected sample of 123 sources that already have complete low and high resolution mid-infrared spectra between 5 microns and 35 microns from the Spitzer Infrared Spectrograph, and which also have spectral energy distributions (SEDs) from IRAS and Akari photometry. [CII] 158 um is the strongest far-infrared emission line and therefore crucial to compare with other features in luminous, dusty galaxies. Sources have 0.004 < z < 0.34 and 43.0 < log L(IR) < 46.8 (erg per sec) and cover the full range of starburst galaxy and AGN classifications. Obtaining these [CII] line fluxes with PACS will allow: 1. determining how precisely [CII] luminosity measures star formation rate by comparing to PAH features and emission lines that arise in starburst galaxies; 2. determining how [CII] luminosity and equivalent width changes with starburst/AGN fraction, by comparing with strength and equivalent width of PAH and [NeII] emission arising from starbursts, and with strength of high ionization lines [NeV] and [OIV] and silicate absorption or emission arising from AGN; 3. determining how [CII] luminosity and equivalent width changes with dust temperature and bolometric luminosity, as derived from spectral energy distributions, and whether this depends on the starburst/AGN fraction. These determinations will allow interpretation of high redshift sources for which the only available diagnostics are the luminosity and equivalent width of the [CII] line and the far-infrared rest-frame SED. The total observing program requires 20.2 hours of Herschel observing time.

  1. ICT & OTs: a model of information and communications technology acceptance and utilisation by occupational therapists (part 2).

    PubMed

    Schaper, Louise; Pervan, Graham

    2007-01-01

    The research reported in this paper describes the development, empirical validation and analysis of a model of technology acceptance by Australian occupational therapists. The study described involved the collection of quantitative data through a national survey. The theoretical significance of this work is that it uses a thoroughly constructed research model, with one of the largest sample sizes ever tested (n=1605), to extend technology acceptance research into the health sector. Results provide strong support for the model. This work reveals the complexity of the constructs and relationships that influence technology acceptance and highlights the need to include sociotechnical and system issues in studies of technology acceptance in healthcare to improve information system implementation success in this arena. The results of this study have practical and theoretical implications for health informaticians and researchers in the field of health informatics and information systems, tertiary educators, Commonwealth and State Governments and the allied health professions.

  2. Topographic form of the Coast Ranges of the Cascadia Margin in relation ot coastal uplift rates and plate subduction

    NASA Technical Reports Server (NTRS)

    Kelsey, Harvey M.; Engebretson, David C.; Mitchell, Clifton E.; Ticknor, Robert L.

    1994-01-01

    The Coast Ranges of the Cascadia margin are overriding the subducted Juan de Fuca/Gorda plate. We investigate the extent to which the latitudinal change in attributes related to the subduction process. These attributes include the varibale age of the subducted slab that underlies the Coast Ranges and average vertical crustal velocities of the western margin of the Coast Rnages for two markedly different time periods, the last 45 years and the last 100 kyr. These vertical crustal velocities are computed from the resurveying of highway bech marks and from the present elevation of shore platforms that have been uplifted in the late Quaternary, respectively. Topogarphy of the Coast Ranges is in part a function of the age and bouyancy of the underlying subducted plate. This is evident in the fact that the two highest topographic elements of the Coast Rnages, the Klamath Mountains and the Olympic Mountains, are underlain by youngest subducted oceanic crust. The subducted Blanco Fracture Zone in southernmost Oregon is responsible for an age discontinuity of subducted crust under the Klamath Mountains. The norhtern terminus of hte topographically higher Klamaths is offset to the north relative to the position of the underlying Blanco Fracture Zone, teh offset being in the direction of migration of the farcture zone, as dictated by relative plate motions. Vertical crustal velocities at the coast, derived from becnh mark surveys, are as much as an order of magnitude greater than vertical crustal velocities derived from uplifted shore platforms. This uplift rate discrepancy indicates that strain is accumulating on the plate margin, to be released during the next interplate earthquake. In a latitudinal sense, average Coast Rnage topography is relatively high where bench mark-derived, short-term vertical crustal velocities are highest. Becuase the shore platform vertical crustal velocities reflect longer-term, premanent uplift, we infer that a small percentage of the interseismic strain that accumulates as rapid short-term uplift is not recovered by subduction earthquakes but rather contributes to rock uplift of the Coast Ranges. The conjecture that permanent rock uplift is related to interseismic uplift is consistent with the observation that those segments of the subduction zone subject to greater interseismic uplift rates are at approximately the same latitudes as those segments of the Coast Ranges that have higher magnitudes of rock uplift over the long term.

  3. OT2_vkulkarn_3: Star Formation and Molecular Gas in Distant Galaxies: SPIRE Spectroscopy of Quasar Absorption Systems

    NASA Astrophysics Data System (ADS)

    Kulkarni, V.

    2011-09-01

    Absorption line systems in quasar spectra, especially the damped Lyman alpha (DLA) and sub-DLA absorbers, provide excellent venues for directly studying the interstellar medium (ISM) in distant galaxies, selected independently of the galaxy luminosities. DLAs/sub-DLAs provide most of the neutral gas reservoir for star formation at high redshifts. A few especially cold, dusty absorbers have been discovered using radio surveys and the Sloan Digital Sky Survey. These absorbers, far richer in dust/molecules than the general absorber population, give us rare opportunities to probe molecular gas and star formation at high redshift. Unfortunately, very few sub-mm observations exist for these unique quasar absorbers. Here we propose SPIRE spectroscopy of 5 quasars with strong absorbers that appear to have cold/dusty gas. The proposed data will efficiently cover a wide spectral range that is expected to be rich in transitions of many atomic and molecular species (e.g., C I, N II, CH+, CO, 13CO, C18O, H2O) at the absorber redshifts. These transitions will allow us to estimate molecular abundances, and physical conditions of the absorber gas such as temperature and density. Comparisons of these distant absorbers with Milky Way ISM will provide a step toward understanding how ISM evolves with time. The molecular lines will also give constraints on isotopic ratios such as 12CO/13CO, and the cosmic microwave background temperature at the absorber redshifts. Our data will also cover the redshifted [C II] 158 micron emission line, which can help to constrain the star formation rate in the absorber galaxies. The proposed data will thus provide several fresh insights into the stellar and interstellar content of distant galaxies, and pave the way for future ALMA observations. Additionally, the data will provide important constraints on the continuum SEDs of the background quasars. Herschel SPIRE is the only current instrument that can offer the wavelength coverage needed to efficiently observe the lines of interest.

  4. OT1_mthomp01_1: Confirming a sample of luminous debris disk candidates identified in the Herschel ATLAS

    NASA Astrophysics Data System (ADS)

    Thompson, M.

    2010-07-01

    Debris disks are the remains of planetary system formation, tracing the existence of planetesimal-sized objects in orbit around main sequence stars. Current and planned surveys of debris disks (including the Herschel Key Projects DEBRIS and DUNES) are deep surveys aimed at characterising the typical population of disks and targeted at samples of a few hundred nearby objects. These deep narrow surveys are relatively insensitive to the rarities in the debris disk population, some of which may be luminous and/or massive disks that have undergone recent disruptive collisional events. We have recently shown that the primarily extragalactic Key Project, the Herschel-ATLAS, can be used as a wide and shallow survey of debris disks by combining its excellent optical coverage and statistical techniques more commonly employed to identify galaxies. The combination of Herschel-ATLAS, DEBRIS and DUNES thus forms a powerful nested tier of surveys that will be sensitive to disks across the spectrum from exosolar analogues to rare disks that cannot be inferred from local populations. In this proposal we seek time to image the three candidate disks that we discovered in the Herschel-ATLAS Science Demonstration Phase with PACS so that we may confirm them as true debris disks and model their SEDs to extract mass, temperature and fractional luminosity. We will confirm whether these disk candidates are in fact the most luminous disks yet detected.

  5. OT2_lyan_4: Investigate the Physical Nature of An Extraordinarily Far-IR bright Galaxy Discovered by WISE

    NASA Astrophysics Data System (ADS)

    Yan, L.

    2011-09-01

    We request a total of 5.3 hours of Herschel PACS and SPIRE time to investigate the physical nature of an extraordinarily far-IR bright system discovered by the WISE data. This object was first selected to have an extremely red mid-IR SED, very bright at 22micron (38mJy) and not detected at WISE 3.4 and 4.5 micron. At the WISE 12micron position, we found it has IRAS far-IR emission, (600+-59)mJy & (855+-170)mJy at 60 and 100 micron respectively (2' beam). A small 350 micron map, taken with the CSO SHARCII under an exceptionally good weather condition, has detected a 32mJy source at the WISE position. The large discrepancy between fluxes at IRAS 100 micron and CSO 350 micron is intriguing. We propose to clarify the nature of this system --- whether a single or a group of far-IR bright sources --- by utilizing Herschel's high spatial resolution and fast mapping speed. The Herschel photometry, together with the Keck spectra, will allow us to determine the full IR SEDs and the physical parameters such as IR luminositie, SFR and star formation efficiecy. In addition, the deep Keck LRIS spectrum of the galaxy closest to the WISE 12 micron position has found an emission line object at redshift of 0.593, and the Keck NIRC2 K-band Adaptive Optics image shows an extended, disk like galaxy. At z=0.593, the strong IRAS far-IR emission implies very high L(FIR), in the range of (2.1-10)e+12Lsun, with the lower limit set by considering only 1/4 of the IRAS fluxes. We propose to obtain SPIRE FTS spectroscopy to measure [CII]158um line. At this redshift, this line can only be observed by Herschel, and is outside the ALMA wavelength window. With the measured [CII]-to-L(FIR) ratio, we can constraint the molecular gas mass of Source A by utilizing the correlation between [CII]-to-L(FIR) and L(FIR)/M(H2). Even with null detection, our observation will provide meaningful constraint on the ISM properties of this ULIRG.

  6. OT2_jpineda_2: Large-scale dynamics and the formation of clouds and stars in the 30 Doradus region of the Large Magellanic Cloud

    NASA Astrophysics Data System (ADS)

    Pineda, J.

    2011-09-01

    Understanding the processes governing the formation of clouds and stars in merging systems is key for the study of how galaxies evolved in the early Universe. The 30 Doradus region in the low-metallicity Large Magellanic Cloud (LMC) is the nearest example of this process, resulting from the interaction between the LMC and the halo of the Milky Way. This makes 30 Doradus the prime laboratory to study these large-scale dynamical processes under conditions that are similar to those at early cosmological times. We propose to use Herschel to obtain a large-scale uniform sampling of this region in [CII] 158um, [NII] 122um and 205um, and [OI] 63um and 146um lines with PACS, and at selected positions in [CII] with HIFI. With this data we will derive the large-scale distribution of the density and pressure of the low-metallicity gas revealing the characteristic signatures of shocked gas. This will then be used to determine the relationships among the diffuse, "dark H2", and dense molecular gas in the 30 Doradus region. We will also derive the electron density distribution of the gas and from this the contribution from ionized gas to the observed [CII] emission. The proposed observations will allow us to study the effect of large-scale gas compression in the multiphase, low-metallicity interstellar medium of 30 Doradus. This information will be valuable for the interpretation of future observations of [CII] in high-redshift galaxies made with ALMA

  7. OT2_jforbric_3: Probing short-term far-infrared variability of protostars and exploring afterglows of X-ray disk heating

    NASA Astrophysics Data System (ADS)

    Forbrich, J.

    2011-09-01

    Recent studies have shown that there is a surprising amount of variability on many timescales in the mid-infrared emission of young stellar objects (YSOs). Especially on short timescales of minutes to hours, mid- and particularly far-infrared variability is currently almost entirely unexplored. While such variability is probing circumstellar disks and envelopes, it is linked to physical processes on the central object. Already the earliest evolutionary stages of YSOs are also strong X-ray emitters, and one important aspect is the heating and possible eventual dispersal of circumstellar disks due to X-ray emission. Even though very important for the understanding of planet formation, this process is poorly understood. The Herschel Space Observatory offers the last possibility for the foreseeable future to explore these processes in more detail and learn about disk heating and explore the occurrence of short-term variability. Here, we propose to do both by repeated observations of the extensively studied nearby Coronet cluster in the CrA star-forming region. Five epochs of near-simultaneous XMM-Newton and Herschel observations, each about 1.5h in duration and spaced by timescales of days to weeks, are meant to explore the short-term variability and link it to the more frequently studied variability on longer timescales. During each epoch, the cluster is mapped 18 times with Herschel. The Coronet has been chosen for having YSOs that can be detected at high S/N in short periods of time while not being too crowded for the angular resolution of both observatories. By exploring far-infrared variability on timescales of several minutes to days and weeks as well as disk heating by protostellar X-ray emission, these short observations will have a unique legacy value.

  8. Laboratory and outside the laboratory measurements of ginned and ot ginned cotton for fiber micronaire and maturity by portable Near Infrared (NIR) Instruments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Micronaire is a key cotton quality assessment property, impacting downstream fiber processing and dye consistency. A component of micronaire is fiber maturity (degree of secondary wall development). Historically, micronaire and maturity are measured in a laboratory under tight environmental condit...

  9. INTERACTION ANALYSES OF BINARY MIXTURES OF CARCINOGENIC PAHS USING MORPHOLOGICAL CELL TRANSFORMATION OF C3H1OT1/2CL8 MOUSE EMBRYO FIBROBLASTS IN CULTURE.

    EPA Science Inventory

    Studies of defined mixtures of carcinogenic polycyclic aromatic hydrocarbons (PAH) have shown three major categories of interactions: antagonism, synergism, and additivity depending on the biological model, tissue, route of exposure, and specific PAH. To understand the bases of t...

  10. Hypersonic aeroheating test of space shuttle vehicle: Configuration 3 (model 22 OTS) in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH20), volume 2

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.; Lockman, W. K.

    1975-01-01

    The model tested was an 0.0175-scale version of the vehicle 3 space shuttle configuration. Temperature measurements were made on the launch configuration, orbiter plus tank, orbiter alone, tank alone, and solid rocket booster (SRB) alone to provide heat transfer data. The test was conducted at free stream Mach numbers of 5.3 and 7.3 and at free stream Reynolds numbers of 1.5, 3.7, 5.0, and 7.0 million per foot. The model was tested at angles of attack from -5 deg to 20 deg and side slip angles of -5 deg and 0 deg.

  11. Method of Transfer from Logical Schemes of Algorithms to Boolean Functions Ot Logicheskikh Skhem Algoritmov K Bulevym Funktsiyam<

    NASA Technical Reports Server (NTRS)

    Dyachenko, V. F.

    1964-01-01

    The author discusses a method for obtaining the Boolean functions describing the structure of a control circuit by means of AS operators whose sequence of operations is given in the form of a logical scheme or matrix scheme for an algorithm. An example of AS synthesis is given.

  12. Fragmentation of the lamellae and fractionation of polymer coils upon mixing poly(dimethylacrylamide) with the lamellar phase of aerosol OT in water.

    PubMed

    Pacios, Isabel E; Renamayor, Carmen S; Horta, Arturo; Lindman, Björn; Thuresson, Krister

    2005-12-22

    The lamellar mesophase formed by surfactant 1,4-bis(2-ethylhexyl) sodium sulfosuccinate (AOT) in deuterated water is mixed with poly(dimethylacrylamide) (PDMAA) polymers of low molecular weight (Mn= (2-20) x 10(3)). The mixtures separate into microphases (lamellar plus isotropic polymer solution). Their microstructures are studied by microscopy, small-angle X-ray scattering (SAXS), and deuterium NMR (2H NMR). According to SAXS, the lamellar phase fractionates the molecular weight distribution of the polymer, by dissolving only chains with coil sizes smaller than the thickness of the water layers between lamellae, and keeping larger chains segregated from the lamellar phase. The fraction of polymer that is segregated from the lamellar phase grows with Mn of the polymer. In 2H NMR, there are two signals, a quadrupolar doublet (water molecules hydrating the anisotropic lamellar phase contribute to this doublet) and a singlet (water molecules in the isotropic polymer solution contribute to this singlet). These two signals are deconvoluted to analyze the phases. Mixing with the polymer produces the partial dispersion of the lamellar phase into small fragments (microcrystallites). The structure of these microcrystallites is such that they conserve the regular long period spacing of the macrophase, and are thus identified in SAXS, but they are smaller than the minimum size required to produce quadrupolar splitting (about 4 microm), and therefore, in 2H NMR, they contribute to the singlet. 2H NMR can thus not distinguish between small microcrystallites and an isotropic polymer solution segregated from the lamellar phase; instead small microcrystallites are detected as an apparent increase of the isotropic solution. The degree of dispersion produced by the polymer in the lamellar phase is correlated with the degree of segregation that the polymer suffers. Thus, much greater dispersion into microcrystallites is produced by the higher Mn polymers than by the lower Mn polymers (in the range covered by the present samples, although with a much higher molecular weight sample (3 x 10(6)) that is totally segregated no such microcrystallites were detected).

  13. Hypersonic aeroheating test of space shuttle vehicle configuration 3 (model 22-OTS) in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH20), volume 1

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.; Lockman, W. K.

    1975-01-01

    The results of hypersonic wind tunnel testing of an 0.0175 scale version of the vehicle 3 space shuttle configuration are presented. Temperature measurements were made on the launch configuration, orbiter plus tank, orbiter alone, tank alone, and solid rocket booster alone to provide heat transfer data. The test was conducted at free-stream Mach numbers of 5.3 and 7.3 and at free-stream Reynolds numbers of 1.5 million, 3.7 million, 5.0 million, and 7.0 million per foot. The model was tested at angles of attack from -5 deg to 20 deg and side slip angles of -5 deg and 0 deg.

  14. Versatile, sensitive liquid chromatography mass spectrometry – Implementation of 10 μm OT columns suitable for small molecules, peptides and proteins

    PubMed Central

    Vehus, T.; Roberg-Larsen, H.; Waaler, J.; Aslaksen, S.; Krauss, S.; Wilson, S. R.; Lundanes, E.

    2016-01-01

    We have designed a versatile and sensitive liquid chromatographic (LC) system, featuring a monolithic trap column and a very narrow (10 μm ID) fused silica open tubular liquid chromatography (OTLC) separation column functionalized with C18-groups, for separating a wide range of molecules (from small metabolites to intact proteins). Compared to today’s capillary/nanoLC approaches, our system provides significantly enhanced sensitivity (up to several orders) with matching or improved separation efficiency, and highly repeatable chromatographic performance. The chemical properties of the trap column and the analytical column were fine-tuned to obtain practical sample loading capacities (above 2 μg), an earlier bottleneck of OTLC. Using the OTLC system (combined with Orbitrap mass spectrometry), we could perform targeted metabolomics of sub-μg amounts of exosomes with 25 attogram detection limit of a breast cancer-related hydroxylated cholesterol. With the same set-up, sensitive bottom-up proteomics (targeted and untargeted) was possible, and high-resolving intact protein analysis. In contrast to state-of-the-art packed columns, our platform performs chromatography with very little dilution and is “fit-for-all”, well suited for comprehensive analysis of limited samples, and has potential as a tool for challenges in diagnostics. PMID:27897190

  15. The Influence of Hydrologic Conditions on the Distribution of Baleen Whales in the Antarctic (Raspredelenie Usatykh Kitov v Anarktike v Zabisimosti ot Gidrologicheskikh Uslovii),

    DTIC Science & Technology

    where a semistationary cyclonic system coincides with plankton rich waters upwelling from great depths. Whales locate these feeding grounds through...growth. They also travel parallel to isohalines to remain in the feeding grounds. (Author)

  16. OT1_sserje01_1: THE HERSCHEL-AKARI NEP DEEP SURVEY: the cosmological history of stellar mass assembly and black hole accretion

    NASA Astrophysics Data System (ADS)

    Serjeant, S.

    2010-07-01

    We propose a far-IR and submm mapping survey of the premier AKARI deep field in the North Ecliptic Pole, in PACS/SPIRE parallel mode. This is the only major deep infrared field not yet covered by Herschel guaranteed or open time key projects. The outstanding and unparalleled continuous mid-IR photometric coverage from AKARI, far better than equivalent Spitzer surveys, enables a wide range of galaxy evolution diagnostics unachievable in any other survey field (including Herschel HerMES/PEP fields), by spanning the wavelengths of redshifted PAH and silicate features and the peak energy output of AGN dust tori. The investment by AKARI in the NEP represents ~10 percent of the entire pointed observations available throughout the lifetime of AKARI. Our proposal remedies the remarkable omission from Herschel's legacy surveys of the premier extragalactic deep field from another IR space telescope. We will simultaneously identify and find photometric redshifts for the Herschel point source population, make stacking analysis detections of the galaxies which dominate the submm extragalactic background light as a function of redshift, determine the bolometric power outputs of the galaxies that dominate the submm background, compare the UV/optical/mid-IR continuum/PAH/far-IR/submm/radio star formation rate estimator in the most comprehensive IR survey data set to date, and track the coupled stellar mass assembly and black hole accretion throughout most of the history of the Universe.

  17. OT2_tdiazsan_1: Size is Not Everything: A PACS Emission Line Study of the Most Compact Infrared-luminous Galaxies

    NASA Astrophysics Data System (ADS)

    Díaz-Santos, T.

    2011-09-01

    Recent results based on far-infrared (IR) data obtained with Herschel strongly suggest the existence of two modes of star formation that apply to low and high-redshift galaxies: a quiescent mode for disks (or main-sequence galaxies) and a starburst mode probably associated with more efficient nuclear, compact star formation. This dichotomy implies that the properties of the inter stellar medium (ISM) in these two types of systems must be substantially different. We have used the mid- to far-IR colors of galaxies as a proxy for their compactness to select a sample of local, compact luminous IR galaxies ((U)LIRGs; LIR >= 10^11 Lsun) from the IRAS 12-micron sample. Our sample of 73 compact (U)LIRGs includes both Seyfert galaxies as well as purely star-forming systems, and therefore is not biased towards active galaxies only. We will observe the key far-IR [CII]158, [OI]63, and [OIII]88 micron emission lines with Herschel/PACS and use models of photo-dissociation regions (PDRs), shocks, X-ray dissociation regions (XDRs), and dusty AGNs to derive the main physical parameters of the ISM in this important class of systems, which are not being targeted by any Herschel project. Our proposed galaxy sample bridges the gap between other studies focused on the analysis of local galaxies with a range of IR luminosities, mid- to far-IR colors, or more spatially extended IR emission, providing a wider view of the star formation and nuclear activity in local IR-bright galaxies in extreme environments, and thus adding a significant contribution to the Herschel legacy. The total time requested for achieving this goal is 89.8 hours.

  18. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... risk, noncomplex holding company); Category II (complex or high risk holding company); or Category III... Holding Company Handbook. (3) A conglomerate is a holding company that: (i) is one of the most complex or... made up of a number of different companies or legal enterprises that offer products from more than...

  19. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... risk, noncomplex holding company); Category II (complex or high risk holding company); or Category III... Holding Company Handbook. (3) A conglomerate is a holding company that: (i) is one of the most complex or... made up of a number of different companies or legal enterprises that offer products from more than...

  20. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... risk, noncomplex holding company); Category II (complex or high risk holding company); or Category III... Holding Company Handbook. (3) A conglomerate is a holding company that: (i) is one of the most complex or... made up of a number of different companies or legal enterprises that offer products from more than...

  1. Subject and authorship of records related to the Organization for Tropical Studies (OTS) in BINABITROP, a comprehensive database about Costa Rican biology.

    PubMed

    Monge-Nájera, Julián; Nielsen-Muñoz, Vanessa; Azofeifa-Mora, Ana Beatriz

    2013-06-01

    BINABITROP is a bibliographical database of more than 38000 records about the ecosystems and organisms of Costa Rica. In contrast with commercial databases, such as Web of Knowledge and Scopus, which exclude most of the scientific journals published in tropical countries, BINABITROP is a comprehensive record of knowledge on the tropical ecosystems and organisms of Costa Rica. We analyzed its contents in three sites (La Selva, Palo Verde and Las Cruces) and recorded scientific field, taxonomic group and authorship. We found that most records dealt with ecology and systematics, and that most authors published only one article in the study period (1963-2011). Most research was published in four journals: Biotropica, Revista de Biología Tropical/ International Journal of Tropical Biology and Conservation, Zootaxa and Brenesia. This may be the first study of a such a comprehensive database for any case of tropical biology literature.

  2. OT2_lolmi_3: The physical and chemical state of high-mass pre- and proto-stellar cores identified by the Hi-GAL survey

    NASA Astrophysics Data System (ADS)

    Olmi, L.

    2011-09-01

    We propose to investigate the physical, chemical and dynamical properties of a sample of newly found high-mass pre- and proto-stellar cores identified by the ``Herschel infrared GALactic Plane Survey'' (Hi-GAL; Molinari et al. 2010). We will perform observations of the o-NH3(1_0-0_0) line (and, simultaneously, also of the o-H2O(1_10-1_01) transition) that will allow us to identify both similarities and differences between the physical and chemical conditions before and after the formation of a warm/hot source inside the dusty core identified in the SPIRE/PACS maps. Hence, these observations will improve our knowledge of the earliest phases in the evolution of high-mass stars. We will also attempt the detection of the NH(1-0) line towards a smaller sample of sources, in order to improve our knowledge of the N-chemistry during early high-mass star formation.

  3. Wind tunnel test of the 0.019 scale space shuttle integrated vehicle (model 14-OTS) in the CALSPAN 8-foot transonic wind tunnel (IA36), volume 2

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    A test is presented which was performed to determine the effect of cold jet gas plumes generated from main propulsion system and solid rocket motor nozzles on: (1) six-component force and moment data, (2) wing static pressures, (3) wing hinge moment, (4) elevon hinge moment, (5) rudder hinge moment, and (6) orbiter MPS nozzle pressure loads. The effects of rudder deflection, nozzle gimbal angle, and plume size were also obtained.

  4. OT2_rivison_2: Characterising the ISM of bright, lensed star-forming galaxies across cosmic time with the SPIRE FTS

    NASA Astrophysics Data System (ADS)

    Ivison, R.

    2011-09-01

    We have shown that SPIRE is capable of exploring high-redshift galaxies spectroscopically, provided those galaxies are sufficiently bright. Here, we propose to exploit its wide wavelength coverage to study the powerful diagnostic rest-frame FIR cooling lines from a sample of 48 bright, lensed - but intrinsically typical - submm galaxies (SMGs). Our targets span 1 < z < 3.1 and 11.5 < log L(FIR) < 13.5, and are selected from panoramic Herschel imaging surveys that are uniquely capable of providing a large, reliable sample at S(350um) > 200mJy, with excellent ancillary data. We will detect or place sensitive limits on key atomic and ionic cooling lines, e.g. [C II], [O I], [O III], and combine these with ground-based observations of 12CO, 13CO, C I and dense-gas tracers to perform a detailed analysis of their ISM and thence understand their energetics and temporal evolution. Using these data we will: 1) empirically constrain the interplay between gas cooling and heating in IR galaxies, mapping the evolution of the star-formation efficiency, exploiting our large sample to separate dependencies on L(FIR) and z, and thereby establishing fundamental relationships for the IR galaxy population; 2) conclusively address the issue of the contribution of AGN to IR galaxies; 3) coadd spectra in the rest frame to delve up to sqrt(48)x deeper than an individual spectrum, to confirm/quantify the signature of powerful feedback via OH molecular outflows, and add powerful diagnostics, e.g. H2O, [O I] and high-J lines, allowing a complete characterisation of the entire FIR spectrum. Goals 1 and 3 drive the requirement for a sample of 48 SMGs. All our goals require Herschel and cannot be addressed by other facilities. We stress that the scientific legacy of ISO and Spitzer has in large part been based on the wealth of data in their spectroscopic archives and the same will likely be true for Herschel.

  5. OT1_rivison_1: Characterising the ISM of bright, lensed star-forming galaxies across cosmic time with the SPIRE FTS

    NASA Astrophysics Data System (ADS)

    Ivison, R.

    2010-07-01

    We have shown that Herschel is capable of exploring high-redshift galaxies spectroscopically, provided those galaxies are sufficiently bright. Here, we propose to exploit the wide wavelength coverage of the SPIRE FTS to study the powerful diagnostic rest-frame FIR cooling lines from a unique and complete sample of 25 bright, gravitationally-lensed - but intrinsically typical - submm galaxies (SMGs). We can thus perform the first detailed analysis of their ISM, tracing their density structure and searching for variations in line strengths compared to local counterparts. Our targets span 1 < z < 3.1 (where [C II] is not accessible to ALMA) and a good range of L(FIR) (12 < log L(FIR) < 13.5), and are selected from panoramic Herschel imaging surveys that are uniquely capable of providing a large, reliable sample at S(350um) > 200mJy, with excellent ancillary data. We will detect or place sensitive limits on the key atomic cooling lines, e.g. [C II], [O I], [O III], and combine these with ground-based observations of 12CO, 13CO, C I and dense-gas tracers to model their ISM and thence understand their energetics and evolution. Using these data we will: 1) map the evolution of the gas content as a function of redshift, via the sensitivity of [C II]/L(FIR) to M(H2); 2) search for changes in the properties of the star-forming gas as a function of redshift and L(FIR); 3) coadd the spectra in the rest frame to delve up to 5x deeper still, to search for faint lines, e.g. H2O and [O I]145.5, allowing a complete characterisation of the average emission; 4) conclusively address the issue of the contribution of AGN to the immense luminosities of submm galaxies. Goals 1-3 drive the requirement for a sample of 25 SMGs. All our goals require Herschel and cannot be addressed by other facilities. We stress that the scientific legacy of ISO and Spitzer has in large part been based on the wealth of data in their spectroscopic archives and the same is likely to be true for Herschel.

  6. OT2_eibar_1: A detailed anaysis of the [CII] line emission for a large sample of star-forming galaxies at z<0.2

    NASA Astrophysics Data System (ADS)

    Ibar, E.

    2011-09-01

    We propose to exploit the spectroscopic capabilities of PACS to describe the [C II] line emission in a unique and comprehensive sample of star-forming galaxies selected from the wide-field, parallel PACS+SPIRE H-ATLAS imaging survey. The sample has exquisite optical spectra from GAMA and SDSS, allowing us to: [1] describe [C II] line as a function of dust and stellar mass, metallicity, extinction, dust temperature, and many other physical parameters; [2] identify the parameters controlling the behaviour of [CII]/L(FIR) at log L(FIR)>11; [3] calibrate [CII] as a star-formation indicator [exploiting our accurate L(FIR) and L(Halpha)] and determine the range over which it is valid. [CII] is potentially an unrivalled tracer of the total gas mass in galaxies (in theory better than CO), and it is therefore an increasingly important observable, e.g. for upcoming ALMA observations of distant galaxies. Our study will become the benchmark for the interpretation of high-z observations, with a legacy value that will survive well into the SPICA era. Some of the key advantages of this proposal over previous Herschel studies such as SHINING and HerCULES are: - we cover 10.2 < log L(FIR) < 11.5 and are unbiased towards powerful ULIRGs with complex merger morphologies; - our sample is selected blindly from H-ATLAS rather than from IRAS, and thus allows exploration of comprehensive parameter space and is much less biased towards galaxies with warm dust emission; - we know the spatial extent of the galaxies, allowing reliable flux measurements via a single pointing within 10 min/target. We can thus achieve our goals in a systematic fashion, maximising the parameter space for the diagnostics of interest. We stress that the scientific legacy of ISO and Spitzer has in large part been based on the wealth of data in their spectroscopic archives and the same will likely be true for Herschel.

  7. MASTER-SAAO follow-up to Fermi-LAT detection of FSRQ PKS B1035-281 activity. And OTs

    NASA Astrophysics Data System (ADS)

    Lipunov, V. M.; Buckley, D. A. H.; Shurpakov, S.; Balanutsa, P.; Gress, O.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Vladimirov, V.; Kornilov, V.; Vlasenko, D.; Gorbunov, I.; Popova, E.; Kuvshinov, D.; Kniazev, A.; Potter, S. B.; Kotze, M.; Rebolo, R.; Ricart, M. Serra; Israelian, G.; Lodieu, N.

    2016-02-01

    MASTER-SAAO auto-detection system (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) detected the brightening of the FSRQ PKS B1035-281 (RA=10 37 42.457 -28 23 04.11) during inspection of Fermi-LAT Detection of its unusual hard spectrum (Carpenter et al., ATEL #8740).

  8. GENERAL REGULARITIES OF FORMATION OF CRYSTAL STRUCTURES OF ELEMENTS DEPENDING ON ELECTRON STRUCTURE OF ATOMS (OBSHCHILE ZAKONOMERNOSTI OBRAZOVANIYA KRISTALLICHESKIKH STRUKTUR ELEMENTOV V ZAVISIMOSTI OT ELEKTRONNOGO STROENIYA ATOMOV),

    DTIC Science & Technology

    A table is given showing the dependence of crystalline structures of elements on the number of electrons in the incompleted electronic subshells of...its atoms. The conclusion is drawn that the types of crystalline structures of the elements depend on the configuration of the outer electronic...subshells acquired by the atoms prior to crystallization. Crystalline structures of elements not governed by the 8 - N rule are determined by one or both

  9. INDUCTION OF GENOTOXIC DAMAGE IS NOT HIGHLY CORRELATED WITH THE ABILITY OT METHYLATE ARSENITE IN VITRO IN THE LEUKOCYTES OF 4 MAMMALIAN SPECIES

    EPA Science Inventory


    Abstract

    Arsenic is a natural drinking water contaminant that impacts the health of large populations of people throughout the world; however, the mode or mechanism by which arsenic induces cancer is unclear. In a series of in vitro studies, we exposed leukocytes from...

  10. Crystallization and preliminary X-ray crystallographic analysis of a conserved domain in plants and prokaryotes from Pyrococcus horikoshii OT3

    SciTech Connect

    Lin, Linyen; Nakano, Hiroaki; Uchiyama, Susumu; Fujimoto, Satoru; Matsunaga, Sachihiro; Nakamura, Shota; Kobayashi, Yuji; Ohkubo, Tadayasu; Fukui, Kiichi

    2005-04-01

    A plant- and prokaryote-conserved domain (PPC) has been crystallized. The crystal diffracted to 1.7 Å resolution and belonged to space group P6{sub 3}22. A plant- and prokaryote-conserved domain (PPC) has previously been found in AT-hook motif nuclear localized protein 1 (AHL1) localized in the nuclear matrix of Arabidopsis thaliana (AtAHL1). AtAHL1 has a DNA-binding function. Mutation analyses of AtAHL1 has previously revealed that the hydrophobic region of the PPC domain is essential for its nuclear localization. In this study, the PPC of the hyperthermophilic archaebacterium Pyrococcus horikoshii (PhPPC) was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the hexagonal space group P6{sub 3}22, with unit-cell parameters a = b = 53.69, c = 159.2 Å. Data were obtained at 100 K, with diffraction being observed to a resolution of 1.7 Å. A complete data set from crystals of the SeMet-substituted protein was also obtained.

  11. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  12. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  13. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  14. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use...

  15. [Characteristics of floor litter and soil arthropod community in different types ot subtropical forest in Ailao Mountain of Yunnan, Southwest China].

    PubMed

    Yang, Zhao; Yang, Xiao-Dong

    2011-11-01

    By using line transect method, an investigation was conducted on the floor litter and soil arthropod community in a mid mountain wet evergreen broad-leaved forest, a mossy dwarf forest, and a Populus bonatii forest in Ailao Mountain of Yunnan in April (dry and hot season), June (rainy season), and December (dry and cold season), 2005. In both dry and rainy seasons, the existing floor litter mass, C storage, and C/N ratio in the three forests all increased in the order of mossy dwarf forest > P. bonatii forest > evergreen broad-leaved forest, but the N storage had less difference. In the floor litter layer of the forests, Acari and Collembola were the dominant groups of soil arthropod community, while Diptera larvae, Coleoptera, ants, and Homoptera were the common groups. The Sorenson coefficients of soil arthropod community in the three forests were extremely great. No significant differences were observed in the soil arthropod density (ind x m(-2)) in the floor litter layer among the three forests, but the relative density (ind x g(-1)) of soil arthropods was higher in the evergreen broad-leaved forest and P. bonatii forest than in the mossy dwarf forest. In the three forests, the density of soil arthropods was significantly higher in dry season than in rainy season, but the Shannon diversity index had less difference. There were significant positive correlations between the existing floor litter mass and the individual density (ind x m(-2)) and dominant groups of soil arthropod communities in dry and hot season (April), but negative correlations between the existing floor litter mass and the relative density (ind x g(-1)) of soil arthropod communities and Acari in dry and cold season (December). The individual densities of Collembola and Coleoptera also had positive correlations with the N storage of the existing floor litter mass in the three forests. It was considered that the floor litter and the development of soil arthropod community in the litter layer of the subtropical forests in Ailao Mountain had a close relation with the vegetation structure of the forests, and the individual density and the diversity of the soil arthropod community were controlled by the floor litter, whereas the environmental factors such as temperature and moisture in the forests also had obvious effects on the seasonal dynamics of the individual density of the soil arthropods.

  16. Aerodynamic results of wind tunnel separation tests on a 0.01-scale model (32-OTS) space shuttle integrated vehicle in the AEDC-VKF-SWTA (IA57)

    NASA Technical Reports Server (NTRS)

    Daileda, J. J.

    1974-01-01

    The integrated space shuttle vehicle was tested to determine proximity force and moment data for the orbiter/external tank and solid rocket booster (SRB) with and without separation rockets firing for both single and dual booster runs. Data were obtained at points t = 0 sec, t = 1.25 sec, and t = 2 sec on the nominal SRB separation trajectory.

  17. Treatability Study in Support of Intrinsic Remediation for Site OT 24 at MacDill Air Force Base, Florida. Volume 2

    DTIC Science & Technology

    1997-01-01

    RECORD STABIUZED WATER LEVEL FEET -MO W O A M M. -( S iT t -T r.L TOTAL MONITORING POINT DEPTH 5.5 FEET Intrtnslc Remedistlon TS 0 BLOW DATUM. MacDill...ELEVATION 1’ -Y t., , GROUNO SURFACE ELEVATION ’I, J, DATUM FOR WATER LEVEL MEASUREMENT - A - (S’ SCREEN DIAMLMR & MATERIAL \\ý" . _ . A ,. SLOT SIZE O.c RISFR...Time (Start): I 11 Water Level : Total Depth of Wellt Water Characteristics Color (, 5c14 " -- Clear Odor: None A Moderate Strong Any Films or Immiscible

  18. An investigation of drag reduction fairings on the space shuttle vehicle 5 configuration (model 74-OTS) in the MSFC 14 inch trisonic wind tunnel (FA14)

    NASA Technical Reports Server (NTRS)

    Ramsey, P. E.

    1976-01-01

    An experimental investigation was conducted in the MSFC 14-inch TWT (FA14, TWT 600) to determine the static stability and drag on a 0.004 scale model of the shuttle ascent configuration. The primary objective was to study the possibility of reducing the launch vehicle drag by using Orbiter/ET/SRB fairings, streamlined orbiter fore and aft attach structures, SRB and ET alternative nose configurations, and devices for modifying the flow between the orbiter and ET. The secondary objective was to determine the longitudinal and directional characteristics of the ascent configuration with the most promising of the drag reduction devices installed. Data were obtained for a Mach number range of 0.6 through 4.96 and angles of attack from -5 through 5 degrees at zero degrees side slip angle.

  19. OT2_mthomp01_2: Confirming a sample of massive and luminous debris disks identified in the Herschel-ATLAS

    NASA Astrophysics Data System (ADS)

    Thompson, M.

    2011-09-01

    Debris disks are the remains of planetary system formation, tracing the existence of planetesimal-sized objects in orbit around main sequence stars. Current and planned surveys of debris disks (including the Herschel Key Projects DEBRIS and DUNES) are deep surveys aimed at characterising the typical population of disks and targeted at samples of a few hundred nearby objects. These deep narrow surveys are relatively insensitive to the rarities in the debris disk population, some of which may be luminous and/or massive disks that have undergone recent disruptive collisional events. We have recently shown that the primarily extragalactic Key Project, the Herschel-ATLAS, can be used as a wide and shallow survey of debris disks by combining its excellent optical coverage and statistical techniques more commonly employed to identify galaxies. The combination of Herschel-ATLAS, DEBRIS and DUNES thus forms a powerful nested tier of surveys that will be sensitive to disks across the spectrum from exosolar analogues to rare disks that cannot be inferred from local populations. In this proposal we seek time to image 23 candidate disks that we have discovered in the Herschel-ATLAS with PACS so that we may confirm them as true debris disks and model their SEDs to extract mass, temperature and fractional luminosity. We will confirm whether these disk candidates are in fact the most luminous disks yet detected.

  20. Job Analysis Techniques for Restructuring Health Manpower Education and Training in the Navy Medical Department. Attachment 10. PT/OT QPCB Task Sort for Physical and Occupational Therapy.

    ERIC Educational Resources Information Center

    Technomics, Inc., McLean, VA.

    This publication is Attachment 10 of a set of 16 computer listed QPCB task sorts, by career level, for the entire Hospital Corps and Dental Technician fields. Statistical data are presented in tabular form for a detailed listing of job duties in physical and occupational therapy. (BT)

  1. OT2_mdickins_1: Herschel+CANDELS: Unraveling the physical processes that regulate star formation and AGN activity in ordinary galaxies at z=2

    NASA Astrophysics Data System (ADS)

    Dickinson, M.

    2011-09-01

    The deepest Herschel surveys have established a distinction between steady "main sequence" star formation, whose rate is closely tied to galaxy mass at a given redshift, and elevated starburst activity in ordinary, L*(IR) galaxies at z > 1.5. They have also shown that supermassive black hole growth occurs mainly through secular processes in these main sequence galaxies, and have revealed otherwise obscured AGN missed by the deepest X-ray surveys. However, the physical processes that trigger starbursts, and the relation between star formation, AGN activity, and the structural properties of galaxies at this peak era of galaxy growth, are far from clear. We propose a survey to address these questions by measuring far-infrared luminosities and star formation rates for a large sample of high redshift galaxies with deep, high-resolution near-infrared imaging from the CANDELS HST WFC3 Treasury program. Herschel PACS and SPIRE observations deep enough to detect 700+ L*(IR) galaxies at z > 1.5 will be obtained for the five CANDELS fields and correlated with the detailed morphological and structural properties that only HST/WFC3 imaging can provide. We will establish whether interactions and mergers drive starburst activity, and examine their relevance for fueling AGN activity. The improved statistics will allow a definitive measure of the space density of L*(IR) galaxies at z=2, a basic (and currently very uncertain) demographic quantity for the history of cosmic star formation. The survey will provide a legacy of targets for detailed investigation with ALMA, tripling (beyond GOODS-South alone) the number of ALMA-accessible fields with the deepest Herschel data.

  2. Treatability Study in Support of Intrinsic Remediation for Site OT 24 at MacDill Air Force Base, Florida. Volume 1

    DTIC Science & Technology

    1997-01-01

    years (calendar year 2016) without the implementation of an engineered remedial action. If a source removal technology such as in situ bioventing is...10 6.3.2 Alternative 2-- Bioventing /Biosparging in Source Area, Intrinsic Remediation, and Institutional Controls with Long-Term Groundwater Monitoring...6-16 6.3.3 Alternative 3--Groundwater Extraction, Bioventing /Biosparging in

  3. Space shuttle launch vehicle (13 P-OTS) strut support interference effects study in the Rockwell International 7- by 7-foot trisonic wind tunnel (IA68)

    NASA Technical Reports Server (NTRS)

    Rogge, R. L.

    1974-01-01

    Strut support interference investigations were conducted on an 0.004-(-) scale representation of the space shuttle launch vehicle in order to determine transonic and supersonic model support interference effects for use in a future exhaust plume effects study. Strut configurations were also tested. Orbiter, external tank, and solid rocket booster pressures were recorded at Mach numbers 0.9, 1.2, 1.5, and 2.0. Angle of attack and angle of sideslip were varied between plus or minus 4 degrees in 2 degree increments. Parametric variations consisted only of the strut configurations.

  4. Favorable environments for the occurrence of overshooting tops in tropical cyclones

    NASA Astrophysics Data System (ADS)

    Sun, Liangxiao; Zhuge, Xiaoyong; Wang, Yuan

    2017-04-01

    Based on Multifunctional Transport Satellite data and the infrared window-texture detection algorithm, the level of overshooting top (OT) activity within a tropical cyclone (TC), which is defined as the hourly mean number of OT occurrence, was statistically investigated in the western North Pacific basin for the period 2005-12. Based on the level of OT activity, the samples were divided into OT and non-OT cases or high-activity-OT (HA-OT) and low-activity-OT (LA-OT) cases. The differences in large-scale environmental variables between OT (HA-OT) and non-OT (LA-OT) cases were examined 12 hours prior to the OT occurrence. Statistical analysis showed that environmental differences did exist between the OT and non-OT cases. The OTs were more skewed towards the early stage of the TC life cycle, and mostly concentrated in low latitudes. Meanwhile, a sufficiently deep warm-water layer, large temperature difference between the upper- and lower-level troposphere, large humidity at the middle and upper levels, and large atmospheric instability, were favorable for OT occurrence. The differences in large-scale environmental characteristics between HA-OTs and LA-OTs were not as significant as those between OTs and non-OTs, but the HA-OT samples tended to occur when the vertical shear was weak and the TC intensity was low. Finally, statistical models were designed to predict the OT and HA-OT. When at least three OT (HA-OT) predictor thresholds were satisfied, the Peirce skill score reached a maximum value of 0.49 (0.30).

  5. Orthostatic tremor in progressive supranuclear palsy.

    PubMed

    de Bie, Rob M A; Chen, Robert; Lang, Anthony E

    2007-06-15

    Patients with orthostatic tremor (OT) can be classified as having "primary OT," with or without postural arm tremor but no other abnormal neurological features, or "OT plus." We describe a patient with OT, with postural tremor of the arms and restless legs syndrome (RLS), who developed features typical of progressive supranuclear palsy (PSP). PSP can be accompanied by OT.

  6. 75 FR 34533 - Deposits

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-17

    ... infocollection.comments@ots.treas.gov . OTS will post comments and the related index on the OTS Internet Site at... savings associations under Regulation DD. Regulation DD implements the Truth in Savings Act, part of...

  7. 76 FR 16477 - General Reporting and Recordkeeping by Savings Associations and Savings and Loan Holding Companies

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-23

    ... infocollection.comments@ots.treas.gov . OTS will post comments and the related ] index on the OTS Internet Site... each savings and loan holding company), 12 CFR part 226 (Regulation Z, truth in lending), 12 CFR...

  8. 76 FR 31680 - General Reporting and Recordkeeping by Savings Associations and Savings and Loan Holding Companies

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-01

    ... infocollection.comments@ots.treas.gov . OTS will post comments and the related index on the OTS Internet Site at... (Regulation Z, truth in lending), 12 CFR part 202 (Regulation B, Equal Credit Opportunity Act), 12 CFR...

  9. 76 FR 56094 - Retail Foreign Exchange Transactions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-12

    ... Supervision (OTS) was the appropriate Federal regulatory agency for Federal savings associations. The OTS did... disputes related to retail forex transactions: civil court litigation and arbitration conducted by...

  10. A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.

    PubMed

    Eliava, Marina; Melchior, Meggane; Knobloch-Bollmann, H Sophie; Wahis, Jérôme; da Silva Gouveia, Miriam; Tang, Yan; Ciobanu, Alexandru Cristian; Triana del Rio, Rodrigo; Roth, Lena C; Althammer, Ferdinand; Chavant, Virginie; Goumon, Yannick; Gruber, Tim; Petit-Demoulière, Nathalie; Busnelli, Marta; Chini, Bice; Tan, Linette L; Mitre, Mariela; Froemke, Robert C; Chao, Moses V; Giese, Günter; Sprengel, Rolf; Kuner, Rohini; Poisbeau, Pierrick; Seeburg, Peter H; Stoop, Ron; Charlet, Alexandre; Grinevich, Valery

    2016-03-16

    Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery.

  11. Aerodynamic results of a separation effects test conducted in the AEDC 40 by 40 inch tunnel A facility on the Rockwell International launch configuration 3 (model-OTS) integrated vehicle (IA13), volume 1

    NASA Technical Reports Server (NTRS)

    Campbell, J. H., II

    1975-01-01

    Experimental aerodynamic investigations were conducted from July 5 through July 17, 1973, on a 0.01 scale model. The AEDC captive trajectory system was utilized in conjunction with the tunnel primary sector to obtain grid-type data for external tank abort from the orbiter, and for nominal separation of one solid rocket booster from the orbiter-tank combination. Booster separation was investigated with and without separation motors plume simulation. The plumes were generated by eight M sub j = 2.15 nozzles using a 1500 psia cold air supply. Free stream data were obtained for all models (orbiter, tank, orbiter-tank, and right-hand booster) to provide baselines for evaluation of proximity effects.

  12. Results of Experimental Investigations to Determine External Tank Protuberance Loads Using a 0.03-Scale Model of the Space Shuttle Launch Configuration (Model 47-OTS) in the NASA/ARC Unitary Plan Wind Tunnel, Volume 2

    NASA Technical Reports Server (NTRS)

    Houlihan, S. R.

    1992-01-01

    Data were obtained on a 3-percent model of the Space Shuttle launch vehicle in the NASA/Ames Research Center 11x11-foot and 9x7-foot Unitary Plan Wind Tunnels. This test series has been identified as IA19OA/B and was conducted from 7 Feb. 1980 to 19 Feb. 1980 (IA19OA) and from 17 March 1980 to 19 March 1980 and from 8 May 1980 to 30 May 1980 (IA19OB). The primary test objective was to obtain structural loads on the following external tank protuberances: (1) LO2 feedline; (2) GO2 pressure line; (3) LO2 antigeyser line; (4) GH2 pressure line; (5) LH2 tank cable tray; (6) LO2 tank cable tray; (7) Bipod; (8) ET/SRB cable tray; and (9) Crossbeam/Orbiter cable tray. To fulfill these objectives the following steps were taken: Eight 3-component balances were used to measure forces on various sections of 1 thru 6 above; 315 pressure orifices were distributed over all 9 above items. The LO2 feedline was instrumented with 96 pressure taps and was rotated to four positions to yield 384 pressure measurements. The LO2 antigeyser line was instrumented with 64 pressure taps and was rotated to two positions to yield 128 pressure measurements; Three Chrysler miniature flow direction probes were mounted on a traversing mechanism on the tank upper surface centerline to obtain flow field data between the forward and aft attach structures; and Schlieren photographs and ultraviolet flow photographs were taken at all test conditions. Data from each of the four test phases are presented.

  13. Results of experimental investigations to determine external tank protuberance loads using a 0.03-scale model of the Space Shuttle launch configuration (model 47-OTS) in the NASA/ARC unitary plan wind tunnel, volume 1

    NASA Technical Reports Server (NTRS)

    Houlihan, S. R.

    1992-01-01

    Data were obtained on a 3-percent model of the Space Shuttle launch vehicle in the NASA/Ames Research Center 11x11-foot and 9x7-foot Unitary Plan Wind Tunnels. This test series has been identified as IA190A/B and was conducted from 7 Feb. 1980 to 19 Feb. 1980 (IA190A) and from 17 March 1980 to 19 March 1980 and from 8 May 1980 to 30 May 1980 (IA190B). The primary test objective was to obtain structural loads on the following external tank protuberances: (1) LO2 feedline, (2) GO2 pressure line, (3) LO2 antigeyser line, (4) GH2 pressure line, (5) LH2 tank cable tray, (6) LO2 tank cable tray, (7) Bipod, (8) ET/SRB cable tray, and (9) Crossbeam/Orbiter cable tray. To fulfill these objectives the following steps were taken: (1) Eight 3-component balances were used to measure forces on various sections of 1 thru 6 above. (2) 315 pressure orifices were distributed over all 9 above items. The LO2 feedline was instrumented with 96 pressure taps and was rotated to four positions to yield 384 pressure measurements. The LO2 antigeyser line was instrumented with 64 pressure taps and was rotated to two positions to yield 128 pressure measurements. (3) Three Chrysler miniature flow direction probes were mounted on a traversing mechanism on the tank upper surface centerline to obtain flow field data between the forward and aft attach structures. (4) Schlieren photographs and ultraviolet flow photographs were taken at all test conditions. Data from each of the four test phases are presented.

  14. Results of transonic wind tunnel tests on an 0.015-scale space shuttle mated vehicle model (67-ots) in the LaRC 8 foot TPT (IA41)

    NASA Technical Reports Server (NTRS)

    Hardin, R.; Burrows, R. R.

    1974-01-01

    Wind tunnel tests were conducted to obtain aerodynamic force data for Mach numbers from 0.60 to 1.20. Data were obtained for an alpha range of -10 deg to +10 deg (beta = 0 deg beta = 5 deg) and beta range of -10 deg to +10 deg (alpha = 0 deg). Longitudinal and lateral-directional stability and control data were obtained for tank alone, tank plus SRB's, tank plus Orbiter, and mated configuration of tank + Orbiter + SRB's. Also, single-component rudder hinge moment data were obtained at rudder deflections of 0 and -20 deg for each Mach number tested. Plots of aerodynamic coefficients vs. Mach number are presented, using data from both test IA41 and tests LRC-UPWT-1056, 1073 (IA42A/B) for Mach numbers of 1.60 to 4.63. The model tested in IA42A/B was the same model as tested in IA41.

  15. OT1_evileniu_1: Probing the extremes of the outer Solar System: short-term variability of the largest, the densest and the most distant TNOs from PACS photometry

    NASA Astrophysics Data System (ADS)

    Vilenius, E.

    2010-07-01

    Pluto was believed to be the outermost object in the Solar System until, at the end of the last Millenium, a number of similar objects were found on orbits beyond Neptune. These objects are now being designated as Trans-Neptunian Objects (TNOs) and are believed to be a reminder of a primordial population. The TNO population comprises a wide range of orbital types and spectral features, many of which are of icy nature. Their origin and evolution are still an open question and subject to debate. We propose repeated high-SNR PACS photometry observations of three extreme specimen of the TNO population: (136199) Eris, the largest dwarf planet, (90377) Sedna, the most distant body in the Solar System and (50000) Quaoar, the densest TNO observed so far. We request a total of 51.7 hours of Herschel observing time using different channels, aiming a SNR of 10 or higher. We intend to perform time-series photometry in order to refine physical properties, which have often been determined using coarse thermal models and single-band data in the past, to find hints of thermal IR lightcurves of Eris and Sedna and to make a high SNR observation of the entire lightcurve of Quaoar. Together with available optical lightcurves we can distinguish whether flux variations are due to shape effects or inhomogeneities of the object's surface. We will make use of existing thermal and thermophysical models to refine physical properties, which may be used to recompute density estimations for Eris and Quaoar and to model surface compositions. Herschel is highly suitable for this task, since it offers the highest sensitivity available to date in the far-IR regime, in which TNO emission peaks due their low surface temperatures. Our results will put constraints on some of the important questions concerning the Trans-Neptunian region and will therefore be of vital importance for observers and modelers in Solar System sciences.

  16. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot transonic wind tunnel, volume 2

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Lemoine, P.

    1992-01-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e., top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  17. Results of heat transfer tests of an 0.0175-scale space shuttle vehicle model 22 OTS in the NASA-Ames 3.5 foot hypersonic wind tunnel (IH3), volume 1

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.

    1975-01-01

    Heat transfer data for the 0.0175-scale space shuttle vehicle 3 are presented. Interference heating effects were investigated by a model build-up technique of orbiter alone, tank alone, second, and first stage configurations. The test program was conducted in the NASA-Ames 3.5-foot hypersonic wind tunnel at Mach 5.3 for nominal free stream Reynolds number per foot values of 1.5, and 5.0 million.

  18. Results of transonic wind tunnel tests on an 0.010-scale space shuttle mated vehicle model 72-OTS in the LaRC 8-foot TPT (IA43)

    NASA Technical Reports Server (NTRS)

    Petrozzi, M. T.; Milam, M. D.

    1975-01-01

    Experimental aerodynamic investigations were conducted in NASA/Langley 8-Foot transonic pressure tunnel on a sting mounted 0.010-scale outer mold line model of 104A/B configuration of the Rockwell International space shuttle vehicle. Component aerodynamic force and moment data and base and balance cavity pressures were recorded over an angle of attack range of -10 deg to +10 deg at Mach numbers of 0.6, 0.8, 0.9, 0.98, 1.13, and 1.2. Selected configurations were tested at sideslip angles from -10 deg to +10 deg. For all configurations involving the orbit, wing bending and torsion were measured on the right wing. Inboard elevon setting of 0 deg, +4 deg and +8 deg and outboard settings of 0 deg, +4 deg and +8 deg were tested.

  19. Supersonic tests of an 0.015 scale space shuttle mated vehicle model (67-OTS) in the LaRC UPWT to obtain aerodynamic force data (IA42A/B)

    NASA Technical Reports Server (NTRS)

    Hardin, R.; Burrows, R. R.

    1974-01-01

    Wind tunnel tests were conducted of the NASA/Rockwell 0.015 scale configuration 4 mated space shuttle vehicle. Data were obtained for a range of Mach numbers from 1.60 to 4.63 and angles of attack from minus 10 degrees to plus 10 degrees. A complete model build-up was performed. Longitudinal and lateral directional stability and control data were obtained for the tank alone, tank plus solid rocket boosters, and mated configuration of tank plus orbiter plus solid rocket boosters. Single component rudder hinge moment data were obtained at rudder deflections of zero degrees and minus 20 degrees for each Mach number tested.

  20. Data report for tests on the heat transfer effects of the 0.0175-scale Rockwell International Space Shuttle Vehicle model 22-OT in the AEDC 50-inch B wind tunnel (OH4B), volume 3

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Grifall, W. J.; Martindale, W.

    1975-01-01

    Results of wind tunnel heat transfer tests of 0.0175-scale Rockwell International Space Shuttle Vehicle configurations for orbiter alone, tank alone, and orbiter plus external tank are presented. Body flap shielding of SSME's during simulated entry was also investigated. The tests were conducted at Mach 8 for thirteen Reynolds number per foot values ranging from 0.5 million to 3.72 million.

  1. Data report for tests on the heat transfer effects of the 0.0175 scale Rockwell International Space Shuttle Vehicle model 22-OT in the AEDC 50 inch B wind tunnel (0H4B), volume 1

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Grifall, W. J.; Martindale, W.

    1975-01-01

    Results of wind tunnel heat transfer tests of 0.0175-scale Rockwell International Space Shuttle Vehicle configurations for orbiter alone, tank alone, and orbiter plus external tank are presented. Body flap shielding of SSME's during simulated entry was investigated. The tests were conducted at Mach 8 for thirteen Reynolds number.

  2. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot transonic wind tunnel, volume 2

    NASA Astrophysics Data System (ADS)

    Marroquin, J.; Lemoine, P.

    1992-10-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e., top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  3. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot Transonic wind tunnel (IA613A), volume 1

    NASA Astrophysics Data System (ADS)

    Marroquin, J.; Lemoine, P.

    1992-10-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e. top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  4. OT2_dneufeld_6: Probing the mystery of water vapour in carbon-rich stars: a search for the H2-17O and H2-18O isotopologues of water toward IRC+10216

    NASA Astrophysics Data System (ADS)

    Neufeld, D.

    2011-09-01

    Using the HIFI instrument, we propose to investigate further the puzzling - but widespread appearance of water vapour in carbon-rich stars. Following up on our discovery that water vapour is present in the warm inner envelope of the carbon rich AGB star IRC+10216, we will carry out a sensitive search for the minor isotopologues, H2-17O and H2-18O. The abundances of these species will provide a critical test of competing models for the origin of the water vapour present in the inner envelope. If the production of water vapor is initiated by the photodissociation of CO by UV radiation, as proposed by Decin et al. (2010) and Agndez et al. (2010), then enhancements in the H2-17O/H2-16O and H2-18O/H2-16O ratios are expected; however, if non-equilibrium chemistry initiated by pulsationally-driven shock waves is responsible - an alternative mechanism proposed recently by Cherchneff (2011) - then no such enhancement will be observed.

  5. Wind tunnel tests of an 0.019-scale space shuttle integrated vehicle -2A configuration (model 14-OTS) in the NASA Ames 8 X 7 foot unitary wind tunnel, volume 3. [cold jet gas plumes and pressure distribution

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    Tests were conducted to determine the effects of cold jet gas plumes on (1) the integrated vehicle longitudinal and lateral-directional force data, (2) exposed wing hinge moment, (3) wing pressure distributions, (4) orbiter MPS external pressure distributions, and (5) model base pressures. An investigation was undertaken to determine the similarity between solid and gaseous plumes; fluorescent oil flow visualization studies were also conducted. Tabulated data listings are included.

  6. Heat transfer tests of an 0.006-scale thin-skin space shuttle model (41-OTS) in the Ames 3.5-foot HWT at M equals 5.3 (IH15)

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.; Lockman, W. K.

    1974-01-01

    Data obtained from heat transfer tests of an 0.006-scale space shuttle vehicle in a 3.5-foot hypersonic wind tunnel are presented. The purpose of these tests was to parametrically investigate the ascent heating of the integrated vehicle. Configurations tested were complete for integrated vehicle, orbiter alone, external tank alone, and SRB alone. All configurations were tested with and without transition grit. Testing was conducted at a Mach number of 5.3, and at Reynolds numbers of 2 and 5 million per foot. The angle of attack range varied from 0 to minus 5 degress, execpt for SRB alone, which was tested from minus 5 to 90 degrees. Heat transfer data were obtained from 223 iron-constantan thermocouples attached to thin-skin stainless steel inserts.

  7. Wind tunnel tests of an 0.019-scale space shuttle integrated vehicle -2A configuration (model 14-OTS) in the NASA Ames 8 X 7 foot unitary wind tunnel, volume 2. [cold jet gas plumes and pressure distribution

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    The purpose of the test was to determine the effects of cold jet gas plumes on (1) the integrated vehicle longitudinal and lateral-directional force data, (2) exposed wing hinge moment, (3) wing pressure distributions, (4) orbiter MPS external pressure distributions, and (5) model base pressures. An investigation was undertaken to determine the similarity between solid and gaseous plumes; fluorescent oil flow visualization studies were also conducted. Plotted wing pressure data is tabulated.

  8. Results from investigations in the NASA/MSFC TWT on a .004 scale model space shuttle launch vehicle (model 13P-OTS) to determine gas supply strut effects on model pressure environment (IA53)

    NASA Technical Reports Server (NTRS)

    Garton, W. P.

    1975-01-01

    The Rockwell Space Shuttle Launch Vehicle components were tested in a Trisonic Wind Tunnel. Pressure measurements were made on the aft portion, in the base regions, and on the wing surfaces. Axial force determination were made from the pressure data. Data were recorded with the model at zero degrees angle of attack and sideslip through a Mach number from 0.9 to 3.0. The only configuration changes investigated were strut mounting techniques and gas line fairing location between the external tank and the orbiter.

  9. Base pressure and heat transfer tests of the 0.0225-scale space shuttle plume simulation model (19-OTS) in yawed flight conditions in the NASA-Lewis 10x10-foot supersonic wind tunnel (test IH83)

    NASA Technical Reports Server (NTRS)

    Foust, J. W.

    1979-01-01

    Wind tunnel tests were performed to determine pressures, heat transfer rates, and gas recovery temperatures in the base region of a rocket firing model of the space shuttle integrated vehicle during simulated yawed flight conditions. First and second stage flight of the space shuttle were simulated by firing the main engines in conjunction with the SRB rocket motors or only the SSME's into the continuous tunnel airstream. For the correct rocket plume environment, the simulated altitude pressures were halved to maintain the rocket chamber/altitude pressure ratio. Tunnel freestream Mach numbers from 2.2 to 3.5 were simulated over an altitude range of 60 to 130 thousand feet with varying angle of attack, yaw angle, nozzle gimbal angle and SRB chamber pressure. Gas recovery temperature data derived from nine gas temperature probe runs are presented. The model configuration, instrumentation, test procedures, and data reduction are described.

  10. Results of investigations conducted in the LaRC 8-foot transonic pressure tunnel using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA93), volume 2

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Test procedures, history, and plotted coefficient data are presented for an aero-loads investigation on the updated configuration-5 space shuttle launch vehicle at Mach numbers from 0.600 to 1.205. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed.

  11. Innovative Clean Coal Technology (ICCT). Demonstration of Selective Catalytic Reduction (SCR) technology for the control of nitrogen oxide (NO{sub x}) emissions from high-sulfur coal-fired boilers: Volume 3, Appendices O--T. Final report

    SciTech Connect

    1996-10-01

    Volume 3 contains the following appendices: Appendix O, Second Series-Manual APH Tests; Appendix P, Third Series-Manual APH Tests; Appendix Q, ABB Analysis of Air Preheaters-Final Report; Appendix R, ABB Corrosion Analysis Study; Appendix S, SRI Waste Stream Impacts Study; and Appendix T, Economic Evaluation.

  12. Results of investigations conducted in the LaRC 8-foot transonic pressure tunnel using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA93)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Test procedures, history, and data from the wind tunnel test are presented. Aero-loads were investigated on the updated configuration-5 space shuttle launch vehicle at Mach numbers from 0.600 to 1.205. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested with two different forward orbiter-to-external-tank attach-strut configurations. The entire model was supported by means of a balance mounted in the orbiter through its base and suspended from a sting.

  13. Results of heat transfer tests of a 0.0175-scale space shuttle vehicle 5 model (60-OTS) in the NASA-Ames Research Center 3.5-foot hypersonic wind tunnel (test IH48)

    NASA Technical Reports Server (NTRS)

    Dye, W. H.; Lockman, W. K.

    1976-01-01

    Heat transfer data are presented for a .0175-scale model of the Rockwell International Space Shuttle Vehicle 5. The primary purpose of these tests was to obtain aerodynamic interference heating data on the external tank in the tank alone, second-, and first-stage configurations. Data were also obtained on the Orbiter and solid rocket boosters. Nominal Mach Nos. of 5.2 and 5.3 at nominal freestream unit Reynolds numbers of 1.5 and 5.0 million per foot, respectively, were investigated. Photographs of the tested configurations and test equipment are shown.

  14. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (141-OT) in the Langley Research Center Freon tunnel at M-6 (IH18). [wind tunnel tests

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1976-01-01

    Ascent heating data were obtained at conditions simulating real gas effects at hypersonic Mach numbers. The configurations tested were Orbiter alone, external tank alone, and mated Orbiter and external tank. A boundary layer trip investigation was conducted for all configurations. The test was conducted at Mach 6 and Reynolds number of one half million per foot for 0 deg and -5 deg angle-of-attack. Selected thermocouples were chosen from the Orbiter and external tank to be used for obtaining heat transfer measurements. A maximum of 42 thermocouples could be measured by the facility data acquisition at one time and no attempt was made to record the excess thermocouples located on the model. Photographs of the test configurations are shown.

  15. Results of heat transfer tests of an 0.0175-scale space shuttle vehicle model 22 OTS in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH3), volume 3

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.

    1975-01-01

    Heat-transfer data for the 0.0175-scale space shuttle vehicle 3 are presented, and interference heating effects were investigated by a model build-up technique of the orbiter. The test program was conducted at Mach 5.3 for nominal free-stream Reynolds number per foot values of 1,500,000 and 5,000,000.

  16. Results of investigations of an 0.010-scale 140A/B configuration (model 72-OTS) of the Rockwell International space shuttle orbiter in the NASA/Langley Research Center unitary plan wind tunnel

    NASA Technical Reports Server (NTRS)

    Petrozzi, M. T.; Milam, M. D.

    1975-01-01

    Experimental aerodynamic investigations were conducted in the NASA/Langley unitary plan wind tunnel on a sting mounted 0.010-scale outer mold line model of the 140A/B configuration of the Rockwell International Space Shuttle Vehicle. The primary test objectives were to obtain: (1) six component force and moment data for the mated vehicle at subsonic and transonic conditions, (2) effects of configuration build-up, (3) effects of protuberances, ET/orbiter fairings and attach structures, and (4) elevon deflection effects on wing bending moment. Six component aerodynamic force and moment data and base and balance cavity pressures were recorded over Mach numbers of 1.6, 2.0, 2.5, 2.86, 3.9, and 4.63 at a nominal Reynolds number of 20 to the 6th power per foot. Selected configurations were tested at angles of attack and sideslip from -10 deg to +10 deg. For all configurations involving the orbiter, wing bending, and torsion coefficients were measured on the right wing.

  17. Results of an investigation of Reynolds effects on integrated vehicle elevon hinge moments and wing panel loads obtained with 0.010 scale model 72 OTS in the Rockwell trisonic wind tunnel (IA141)

    NASA Technical Reports Server (NTRS)

    Mennell, R. C.

    1976-01-01

    Wind tunnel investigations were conducted on an 0.010-scale representation of the VL70-000140C Integrated Space Shuttle Launch Vehicle. The primary test objective was to obtain Reynolds number effects on orbiter elevon hinge moments and wing bending/torsional moments. Launch vehicle aerodynamic force data were also recorded. The elevon hinge moments, wing bending/torsional moments, and vehicle force data were recorded over an angle of attack range of -6 deg to +6 deg, an angle of sideslip range of -6 deg to +6 deg, at Mach numbers of 0.6, 0.975, 1.05 and 1.25. The Reynolds number was varied from a minimum of 4.5 million/foot to a maximum of 11.5 million/foot. The complete integrated configuration was tested with the orbiter elevons set at 0 deg and deflected to 9 deg on the outboard elevon and 10 deg on the inboard elevon. Testing was conducted in the TWT 19.7% porous transonic test section with the model sting mounted through the orbiter base. All aerodynamic force data were obtained from internal strain gage balance located in the orbiter.

  18. Aeroheating (pressure) characteristics on a 0.10-scale version of the vehicle 3 space shuttle configuration (26-OTS) in the Langley Research Center 4-foot wind tunnel (IH4)

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.

    1976-01-01

    Results of wind tunnel tests, conducted at the Langley Research Center Unitary Plan Wind Tunnel, are presented. The model tested was an 0.010-scale version of the Vehicle 3 Space Shuttle Configuration. Pressure measurements were made on the launch configuration, Orbiter alone, external tank alone, and solid rocket booster alone, to provide heat transfer pressure data. The tests were conducted for a Mach number range from 2.36 to 4.6 and Reynolds number range from 1.2 to 5 million per foot. The model was tested at angles of attack from -10 to 20 deg for a sideslip angle range from -5 to +5 deg, and at sideslip angles from -5 to 48 deg for 0 deg angle of attack. Tabulated data are given and photographs of the test configuration are shown.

  19. Results of an external tank separation test in AEDC/VKF tunnel B on 0.010-scale replica of space shuttle vehicle model 52-OT(IA17A), Volume 1

    NASA Technical Reports Server (NTRS)

    Spangler, R. H.; Daileda, J. J.

    1975-01-01

    Tests were conducted on scale models of the space shuttle orbiter and external tank (ET) to determine the aerodynamic interactions during a return to launch site abort separation. The orbiter model was built to vehicle 3 configuration lines (139B) and the ET model approximated the vehicle 5 configurations with protuberances and attach hardware. For these investigations the orbiter was mounted on the primary support system and the external tank was mounted on the captive trajectory system. Six-component data were obtained for each vehicle at various orbiter angles of attack and sideslip for a range of relative angular and linear displacements of the ET from the orbiter.

  20. An investigation in MSFC 14-inch TWT to determine the static stability characteristics of 0.004-scale model (74-OTS) space shuttle vehicle 5 configuration (IA33), volume 1

    NASA Technical Reports Server (NTRS)

    Allen, E. C.

    1975-01-01

    Wind tunnel tests were conducted to: (1) determine the static stability characteristics of the Shuttle Vehicle 5 configuration; (2) determine the effect on the Vehicle 5 aerodynamic characteristics of External Tank (ET) and Solid Rocket Booster (SRB) nose shape, SRB nozzle shroud flare angle, orbiter to tank fairing, and sting location; (3) provide flow visualization using thin film oil paint; and (4) determine rudder, body flap, and inboard and outboard elevon hinge moments. The mated vehicle model was mounted in three different ways: (1) the orbiter mounted on the balance with the SRB's attached to the tank and the tank in turn attached to the orbiter; (2) the tank mounted on the balance (with the sting protruding through the tank base) with the SRB's and orbiter attached to the tank, and (3) with the tank mounted on the balance and the balance in turn supported by a forked sting entering the nozzle of each SRB, extending forward into the SRB's then crossing over to the tank to provide a balance socket. Data were obtained for Mach numbers from 0.6 through 4.96 at angles-of-attack and -sideslip from -10 to 10 degrees.

  1. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot Transonic wind tunnel (IA613A), volume 1

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Lemoine, P.

    1992-01-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e. top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  2. Neural Basis of Empathy and Its Dysfunction in Autism Spectrum Disorders (ASD)

    DTIC Science & Technology

    2012-08-01

    then for the first time demonstrated that inhaled OT using a pediatric nebulizer effectively reaches the central nervous system of rhesus macaques...Examine the effect of oxytocin (OT) on task performance We have completed this task. We showed that inhaled OT (using pediatric nebulizer...providing a proof of concept for a clinical OT treatment method well-tolerated by children. Subsequently, we showed the inhaled OT promotes prosocial

  3. Could oxytocin administration during labor contribute to autism and related behavioral disorders?--A look at the literature.

    PubMed

    Wahl, Roy U Rojas

    2004-01-01

    This literature review summarizes recent potential evidence, most of which is at the molecular/mechanistic level, in support of Hollander's hypothesis that excess oxytocin (OT), possibly through OT administration at birth, could contribute to the development of autistic spectrum disorders and related syndromes by proposed down regulation of the OT receptor (OTR). In this review, recent molecular evidence for OTR internalization by excess OT is related to OT's reported effects on animal social behavior, favoring social bondage, notably in sheep, voles, rats and especially mice. Adding indications for OT's capability of crossing the maternal placenta and OT's possibility of crossing an underdeveloped or stressed infantile blood brain barrier at birth, a causal connection between OT excess and behavioral disorders such as autism can be supported from a molecular perspective. Possible strategies such as a thorough statistical analysis of numerous birth records as well as molecular studies such as radiotracing using labeled OT are proposed to test this hypothesis.

  4. Urinary oxytocin as a non-invasive biomarker for neurohypophyseal hormone secretion.

    PubMed

    Polito, Anthony B; Goldstein, David L; Sanchez, Lylian; Cool, David R; Morris, Mariana

    2006-11-01

    The objective was to characterize the urinary oxytocin (OT) system with the goal of using it as a biomarker for neurohypophyseal peptide secretion. We studied urinary OT secretion in mice under three conditions: (1) in OT gene deletion mice (OT -/-) which lack the ability to produce the peptide; (2) after arterial vascular infusion of OT and (3) after physiological stimulation with consumption of 2% sodium chloride. OT was measured by radioimmunoassay (RIA) and Surface-Enhanced Laser Desorption Ionization Time of Flight Mass Spectroscopy (SELDI TOF MS). In OT -/- mice (n=25), urinary OT levels were not detectable, while in OT +/+ mice (n=23) levels were 250.2+/-35.3 pg/ml. To evaluate blood/urine transfer, mice with chronic carotid arterial catheters were infused with saline or OT (5 or 20 pmol/min). Peak urine OT levels were 89+/-11.5 and 844+/-181 ng/ml in the low and high OT groups, respectively. Proteomic evaluation showed MS peaks, corresponding to OT ( approximately 1009 Da) and a related peptide ( approximately 1030 Da) with highest levels in mice infused with OT. Salt loading (5 days of 2% NaCl as drinking water) increased plasma osmolality (3.3%), increased plasma and urinary vasopressin (AVP), but caused no changes in OT. Thus, using non-invasive urine samples, we document that urinary OT and AVP can be used to monitor changes in peptide secretion. Urinary OT and AVP, as well as other urinary peptides, may provide a viable biomarker for peptide secretion and may be useful in clinical studies.

  5. Relationship of plasma oxytocin levels to baseline symptoms and symptom changes during three weeks of daily oxytocin administration in people with schizophrenia.

    PubMed

    Lee, Mary R; Wehring, Heidi J; McMahon, Robert P; Liu, Fang; Linthicum, Jared; Verbalis, Joseph G; Buchanan, Robert W; Strauss, Gregory P; Rubin, Leah H; Kelly, Deanna L

    2016-04-01

    Several clinical studies have found an inverse relationship between clinical symptoms and peripheral oxytocin (OT) levels in people with schizophrenia. As oxytocin is a putative treatment for schizophrenia, the effect of repeated dosing of OT on OT levels, clinical symptoms and the relationship between the two is of interest. In a, randomized, double blind, parallel group 3 week study (N=28) with daily administration of intranasal OT (20 IU twice daily) or placebo (PBO), we examined the effect of OT administration on the correlation between the change in peripheral OT levels and change in clinical symptoms in patients with schizophrenia. At baseline, there were no significant treatment group differences in OT levels. There were no significant associations between baseline OT levels and any symptom measures. After 3 weeks of OT/PBO dosing, there was no significant difference in the magnitude of change in OT levels between the two treatment groups. Correlations between changes in peripheral OT levels and changes in the BPRS total and negative symptom scores were not different between treatment groups. Larger studies are needed to examine the effect of exogenous OT on peripheral OT levels and the relationship between the latter and clinical symptoms. Clinical Trials.gov=NCT00884897.

  6. Does Oxytocin Increase Trust in Humans? A Critical Review of Research.

    PubMed

    Nave, Gideon; Camerer, Colin; McCullough, Michael

    2015-11-01

    Behavioral neuroscientists have shown that the neuropeptide oxytocin (OT) plays a key role in social attachment and affiliation in nonhuman mammals. Inspired by this initial research, many social scientists proceeded to examine the associations of OT with trust in humans over the past decade. To conduct this work, they have (a) examined the effects of exogenous OT increase caused by intranasal administration on trusting behavior, (b) correlated individual difference measures of OT plasma levels with measures of trust, and (c) searched for genetic polymorphisms of the OT receptor gene that might be associated with trust. We discuss the different methods used by OT behavioral researchers and review evidence that links OT to trust in humans. Unfortunately, the simplest promising finding associating intranasal OT with higher trust has not replicated well. Moreover, the plasma OT evidence is flawed by how OT is measured in peripheral bodily fluids. Finally, in recent large-sample studies, researchers failed to find consistent associations of specific OT-related genetic polymorphisms and trust. We conclude that the cumulative evidence does not provide robust convergent evidence that human trust is reliably associated with OT (or caused by it). We end with constructive ideas for improving the robustness and rigor of OT research.

  7. Learning about oxytocin: pharmacologic and behavioral issues.

    PubMed

    Chini, Bice; Leonzino, Marianna; Braida, Daniela; Sala, Mariaelvina

    2014-09-01

    Despite the accumulating evidence suggesting that the neuropeptide oxytocin (OT) plays a role in neuropsychiatric disorders characterized by social dysfunction, the influence of OT on the nonsocial aspects of learning and memory have been less investigated. To foster research in this area, we review the effects of OT on learning and memory in animal models and humans. In healthy animal models, OT improves memory consolidation and extinction, but only if given at a low dose immediately after the acquisition phase. On the contrary, OT effects in healthy humans have been inconsistent; although, in this case, OT was always given before the acquisition phase and no dose-response curves have ever been drawn up. Interestingly, a specific impairment in the reversal of learning has been found in mice devoid of OT receptors and OT has been demonstrated to enhance fear extinction in rodents. All together, these data suggest that OT plays a role in elementary forms of behavioral flexibility and adaptive responses and support its therapeutic potential in neuropsychiatric disorders characterized by cognitive inflexibility and/or impairment (autism, schizophrenia, Alzheimer's disease, Parkinson disease, stroke, posttraumatic stress disorder). Accordingly, OT has been shown to improve cognitive flexibility in OT receptor-deficient mice, and scattered findings indicate that intranasal OT has positive effects on the memory of patients with schizophrenia or posttraumatic stress disorders. Further studies of the therapeutic potential of OT as an enhancer of learning and memory are warranted.

  8. Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight mass spectrometry.

    PubMed

    Fitzgerald, M C; Chernushevich, I; Standing, K G; Whitman, C P; Kent, S B

    1996-07-09

    Electrospray ionization time-of-flight (ESI-TOF) mass spectrometry was used to study the quaternary structure of 4-oxalocrotonate tautomerase (EC 5.3.2; 4OT), and four analogues prepared by total chemical synthesis. Wild-type 4OT is a hexamer of 62 amino acid subunits and contains no cysteine residues. The analogues were: (desPro1)4OT, a truncated construct in which Pro1 was deleted; (Cpc1)4OT in which Pro1 was replaced with cyclopentane carboxylate; a derivative [Met(O)45]4OT in which Met45 was oxidized to the sulfoxide; and an analogue (Nle45)4OT in which Met45 was replaced with norleucine. ESI of (Nle45)4OT, (Cpc1)4OT, and 4OT from solution conditions under which the native enzyme was fully active (5 mM ammonium bicarbonate buffer, pH 7.5) gave the intact hexamer as the major species detected by TOF mass spectrometry. In contrast, analysis of [Met(O)45]4OT and (desPro1)4OT under similar conditions yielded predominantly monomer ions. The ESI-TOF measurements were consistent with structural data obtained from circular dichroism spectroscopy. In the context of kinetic data collected for 4OT and these analogues, ESI-TOF mass spectrometry also provided important evidence for the structural and mechanistic significance of the catalytically important Pro1 residue in 4OT.

  9. Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight mass spectrometry.

    PubMed Central

    Fitzgerald, M C; Chernushevich, I; Standing, K G; Whitman, C P; Kent, S B

    1996-01-01

    Electrospray ionization time-of-flight (ESI-TOF) mass spectrometry was used to study the quaternary structure of 4-oxalocrotonate tautomerase (EC 5.3.2; 4OT), and four analogues prepared by total chemical synthesis. Wild-type 4OT is a hexamer of 62 amino acid subunits and contains no cysteine residues. The analogues were: (desPro1)4OT, a truncated construct in which Pro1 was deleted; (Cpc1)4OT in which Pro1 was replaced with cyclopentane carboxylate; a derivative [Met(O)45]4OT in which Met45 was oxidized to the sulfoxide; and an analogue (Nle45)4OT in which Met45 was replaced with norleucine. ESI of (Nle45)4OT, (Cpc1)4OT, and 4OT from solution conditions under which the native enzyme was fully active (5 mM ammonium bicarbonate buffer, pH 7.5) gave the intact hexamer as the major species detected by TOF mass spectrometry. In contrast, analysis of [Met(O)45]4OT and (desPro1)4OT under similar conditions yielded predominantly monomer ions. The ESI-TOF measurements were consistent with structural data obtained from circular dichroism spectroscopy. In the context of kinetic data collected for 4OT and these analogues, ESI-TOF mass spectrometry also provided important evidence for the structural and mechanistic significance of the catalytically important Pro1 residue in 4OT. PMID:8692908

  10. Secondary orthostatic tremor in the setting of cerebellar degeneration.

    PubMed

    Sarva, Harini; Severt, William Lawrence; Jacoby, Nuri; Pullman, Seth L; Saunders-Pullman, Rachel

    2016-05-01

    Orthostatic tremor (OT) and cerebellar ataxia are uncommon and difficult to treat. We present two patients with OT and cerebellar degeneration, one of whom had spinocerebellar ataxia type 2 and a good treatment response.

  11. 12 CFR 516.1 - What does this part do?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... processing procedures and time frames for the approval of applications. (c) If an OTS regulation for a specific type of application prescribes some application processing procedures, or time frames, OTS...

  12. Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

    PubMed Central

    Brandtzaeg, Ole Kristian; Johnsen, Elin; Roberg-Larsen, Hanne; Seip, Knut Fredrik; MacLean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray

    2016-01-01

    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. PMID:27528413

  13. 76 FR 42768 - Capital Distribution

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ... Office of Thrift Supervision Capital Distribution AGENCY: Office of Thrift Supervision (OTS), Treasury... concerning the following information collection. Title of Proposal: Capital Distribution. OMB Number: 1550..., the information provides the OTS with a mechanism for monitoring capital distributions since...

  14. Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

    NASA Astrophysics Data System (ADS)

    Brandtzaeg, Ole Kristian; Johnsen, Elin; Roberg-Larsen, Hanne; Seip, Knut Fredrik; Maclean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray

    2016-08-01

    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.

  15. Spatio-temporal comparisons of imposex status and tissue organotin concentration in the whelk Reishia clavigera collected along the coasts of Dapeng Bay and Daya Bay, Shenzhen, China.

    PubMed

    Ho, Kevin K Y; Leung, Kenneth M Y

    2014-08-15

    Organotin compounds (OTs) have caused widespread adverse effects on marine organisms. As no local restrictions on OT-based antifouling paints have been implemented in China, high concentrations of OTs still occur in coastal environments. In this study, we measured the imposex status and tissue concentrations of OTs in the whelk Reishia clavigera collected along the coast of Dapeng Bay and Daya Bay of Shenzhen, China in 2013. Our results showed that all female whelks suffered from the onset of imposex. The highest concentration of total OTs was 27,756 μg kg(-1) dry weight in the samples collected from Shuitousha. Triphenyltin was the most predominant residue, accounting for more than 97.8% of total OTs across all sites. Compared with the results from previous studies, the marine environment of this region is still heavily contaminated with OTs. Therefore, a tightened control is necessary to regulate the use and release of OTs in China.

  16. 12 CFR 516.30 - What information must I provide with my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... on OTS's web page at www.ots.treas.gov. (b) Captions and exhibits. You must caption the original... signatures on copies if you include a copy of the signed signature page or the copy otherwise indicates...

  17. Effects of oolong tea on renal sympathetic nerve activity and spontaneous hypertension in rats.

    PubMed

    Tanida, Mamoru; Tsuruoka, Nobuo; Shen, Jiao; Kiso, Yoshinobu; Nagai, Katsuya

    2008-04-01

    In a previous study, evidence was presented that oolong tea (OT) reduced abdominal fat accumulation in diet-induced obese mice. In the study presented here, we examined the sympathetic and cardiovascular effects of intraduodenal injection of OT in urethane-anesthetized rats and found that it suppressed renal sympathetic nerve activity (RSNA) and blood pressure (BP). In addition, pretreatment with the histaminergic H3-receptor antagonist thioperamide or bilateral subdiaphragmatic vagotomy eliminated the effects of OT on RSNA and BP. Furthermore, OT drinking for 14 weeks reduced BP elevation in spontaneously hypertensive rats. These results thus suggest that OT may exert its hypotensive action through changes in autonomic neurotransmission via an afferent neural mechanism. Moreover, we found that intraduodenal injection of decaffeinated OT lowered RSNA and BP as well as OT, indicating that substances other than caffeine contained in OT may function as effective modulators of RSNA and BP.

  18. 75 FR 20041 - Deposits

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-16

    ... e-mail to infocollection.comments@ots.treas.gov . OTS will post comments and the related index on... regulations applicable to savings associations under Regulation DD. Regulation DD implements the Truth...

  19. 75 FR 42833 - Prohibited Service at Savings and Loan Holding Companies

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... to (202) 906-6518, or by e-mail to infocollection.comments@ots.treas.gov . OTS will post comments and... associations under Regulation DD. Regulation DD implements the Truth in Savings Act, part of the...

  20. 12 CFR 552.2-1 - Procedure for organization of Federal stock association.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... will perform a role of providing credit for housing consistent with safe and sound operation of a... after the OTS approves the application, or within such additional period as the OTS for good cause...

  1. Polyfibroblast: A Self-Healing and Galvanic Protection Additive

    DTIC Science & Technology

    2012-03-23

    more OTS is available to passivate the exposed steel. Moisture resistance results comparing last year’s data to this year’s data are given in Figure 1...as an anti- caking agent. GPS-loaded microcapsules appear to cake irreversibly even with the added nanopowder. This discrepancy may be due to the fact...that OTS does not polymerize as readily as IPDI. The OTS-rich microcapsules may break during filtration , but the OTS does not glue the microcapsules

  2. Oxytocin by intranasal and intravenous routes reaches the cerebrospinal fluid in rhesus macaques: determination using a novel oxytocin assay.

    PubMed

    Lee, M R; Scheidweiler, K B; Diao, X X; Akhlaghi, F; Cummins, A; Huestis, M A; Leggio, L; Averbeck, B B

    2017-03-14

    Oxytocin (OT) is a potential treatment for multiple neuropsychiatric disorders. As OT is a peptide, delivery by the intranasal (IN) route is the preferred method in clinical studies. Although studies have shown increased cerebrospinal fluid (CSF) OT levels following IN administration, this does not unequivocably demonstrate that the peripherally administered OT is entering the CSF. For example, it has been suggested that peripheral delivery of OT could lead to central release of endogenous OT. It is also unknown whether the IN route provides for more efficient entry of the peptide into the CSF compared to the intravenous (IV) route, which requires blood-brain barrier penetration. To address these questions, we developed a sensitive and specific quantitative mass spectrometry assay that distinguishes labeled (d5-deuterated) from endogenous (d0) OT. We administered d5 OT (80 IU) to six nonhuman primates via IN and IV routes as well as IN saline as a control condition. We measured plasma and CSF concentrations of administered and endogenous OT before (t=0) and after (t=10, 20, 30, 45 and 60 min) d5 OT dosing. We demonstrate CSF penetrance of d5, exogenous OT delivered by IN and IV administration. Peripheral administration of d5 OT did not lead to increased d0, endogenous OT in the CSF. This suggests that peripheral administration of OT does not lead to central release of endogenous OT. We also did not find that IN administration offered an advantage compared to IV administration with respect to achieving greater CSF concentrations of OT.Molecular Psychiatry advance online publication, 14 March 2017; doi:10.1038/mp.2017.27.

  3. Adolescent oxytocin exposure causes persistent reductions in anxiety and alcohol consumption and enhances sociability in rats.

    PubMed

    Bowen, Michael T; Carson, Dean S; Spiro, Adena; Arnold, Jonathon C; McGregor, Iain S

    2011-01-01

    Previous studies have suggested that administration of oxytocin (OT) can have modulatory effects on social and anxiety-like behavior in mammals that may endure beyond the time of acute OT administration. The current study examined whether repeated administration of OT to male Wistar rats (n = 48) during a key developmental epoch (early adolescence) altered their physiology and behavior in later-life. Group housed rats were given intraperitoneal injections of either 1 mg/kg OT or vehicle during early adolescence (post natal-days [PND] 33-42). OT treatment caused a transient inhibition of body weight gain that recovered quickly after the cessation of treatment. At PND 50, the rats pre-treated with OT displayed less anxiety-like behavior on the emergence test, while at PND 55 they showed greater levels of social interaction. A subgroup of OT pre-treated rats examined at PND 63 showed a strong trend towards increased plasma OT levels, and also displayed significantly increased OT receptor mRNA in the hypothalamus. Rats pre-treated with OT and their controls showed similar induction of beer intake in daily 70 min test sessions (PND 63 onwards) in which the alcohol concentration of beer was gradually increased across days from 0.44% to 4.44%. However, when given ad libitum access to beer in their home cages from PND 72 onwards (early adulthood), consumption of beer but not water was significantly less in the OT pre-treated rats. A "booster" shot of OT (1 mg/kg) given after 25 days of ad libitum access to beer had a strong acute inhibitory effect on beer intake without affecting water intake. Overall these results suggest that exogenous OT administered during adolescence can have subtle yet enduring effects on anxiety, sociability and the motivation to consume alcohol. Such effects may reflect the inherent neuroplasticity of brain OT systems and a feed-forward effect whereby exogenous OT upregulates endogenous OT systems.

  4. Oxytocin reduces separation distress in piglets when given intranasally

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Oxytocin (OT) is one of the neurobiological foundations of sociality. It acts as a neuropeptide in numerous social processes, from ultra-social to anti-social behaviors. Evidence supports a role for OT in social support, possibly by attenuating separation distress. Nonetheless, research on OT is lac...

  5. 76 FR 42767 - Management Officials Interlocks

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ... Office of Thrift Supervision Management Officials Interlocks AGENCY: Office of Thrift Supervision (OTS... Management and Budget (OMB) for review, as required by the Paperwork Reduction Act. Today, OTS is soliciting...: Management Officials Interlocks. OMB Number: 1550-0051. Form Number: N/A. Description: OTS uses the...

  6. 75 FR 41573 - Proposed Agency Information Collection Renewal; Joint Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... change to the OTS Internet Site at http://www.ots.treas.gov/pagehtml.cfm?catNumber=67&an=1 , including... comments received, go to http://www.ots.treas.gov/pagehtml.cfm?catNumber=67&an=1 . In addition, you...

  7. Anatomy in Occupational Therapy Program Curriculum: Practitioners' Perspectives

    ERIC Educational Resources Information Center

    Schofield, Katherine Anne

    2014-01-01

    Anatomy education is undergoing significant transformation. It is unknown whether changes are in accordance with occupational therapy (OT) practice needs. The purpose of this pilot study was to survey OT clinicians to determine their perspectives on the value of anatomy in OT curricula, and anatomical knowledge required for practice. In addition…

  8. Maternal and Paternal Plasma, Salivary, and Urinary Oxytocin and Parent-Infant Synchrony: Considering Stress and Affiliation Components of Human Bonding

    ERIC Educational Resources Information Center

    Feldman, Ruth; Gordon, Ilanit; Zagoory-Sharon, Orna

    2011-01-01

    Studies in mammals have implicated the neuropeptide oxytocin (OT) in processes of bond formation and stress modulation, yet the involvement of OT in human bonding throughout life remains poorly understood. We assessed OT in the plasma, saliva, and urine of 112 mothers and fathers interacting with their 4-6-month-old infants. Parent-infant…

  9. OXYTOCIN INDUCES SOCIAL COMMUNICATION BY ACTIVATING ARGININEVASOPRESSIN V1A RECEPTORS AND NOT OXYTOCIN RECEPTORS

    PubMed Central

    SONG, Zhimin; MCCANN, Katharine E.; MCNEILL, John K.; LARKIN, Tony E.; HUHMAN, Kim L.; ALBERS, H. Elliott

    2014-01-01

    Arginine-vasopressin (AVP) and oxytocin (OT) and their receptors are very similar in structure. As a result, at least some of the effects of these peptides may be the result of crosstalk between their canonical receptors. The present study investigated this hypothesis by determining whether the induction of flank marking, a form of social communication in Syrian hamsters, by OT is mediated by the OT receptor or the AVP V1a receptor. Intracerebroventricular (ICV) injections of OT or AVP induced flank marking in a dose-dependent manner although the effects of AVP were approximately 100 times greater than those of OT. Injections of highly selective V1a receptor agonists but not OT receptor agonists induced flank marking, and V1a receptor antagonists but not OT receptor antagonists significantly inhibited the ability of OT to induce flank marking. Lastly, injection of alpha-melanocyte-stimulating hormone (α-MSH), a peptide that stimulates OT but not AVP release, significantly increased odor-induced flank marking, and these effects were blocked by a V1a receptor antagonist. These data demonstrate that OT induces flank marking by activating AVP V1a and not OT receptors, suggesting that the V1a receptor should be considered to be an OT receptor as well as an AVP receptor. PMID:25173438

  10. Neonatal manipulation of oxytocin alters oxytocin levels in the pituitary of adult rats.

    PubMed

    Young, E; Carter, C S; Cushing, B S; Caldwell, J D

    2005-07-01

    The neuropeptide oxytocin (OT) and its OT antagonists (OTA) in infant rats affect their behavior as adults. In this study we attempted to determine whether treating rats on the day of birth (postnatal day 1) with OT or OTA would affect brain OT levels of these rats as adults. Rat pups were injected with OT (3 microg), OTA (0.3 microg) or saline vehicle ip on postnatal day 1. As 60-day-old adults, treated rats were killed, and the OT content in their medial preoptic areas (MPOAs), medial hypothalami (MH) and pituitaries were assayed. In females, treatment with OTA on postnatal day 1 significantly decreased pituitary OT levels as adults. In males, by contrast, treatment with OTA on postnatal day 1 resulted in increased pituitary OT levels when they become adults compared to male rats treated with OT on postnatal day 1. There were no significant effects of neonatal treatment on OT levels in either the MH or MPOA. Day 1 postnatal treatment with OT or OTA had a long-term sexually dimorphic effect on OT levels in the pituitary.

  11. Ontogenesis of oxytocin pathways in the mammalian brain: late maturation and psychosocial disorders

    PubMed Central

    Grinevich, Valery; Desarménien, Michel G.; Chini, Bice; Tauber, Maithé; Muscatelli, Françoise

    2014-01-01

    Oxytocin (OT), the main neuropeptide of sociality, is expressed in neurons exclusively localized in the hypothalamus. During the last decade, a plethora of neuroendocrine, metabolic, autonomic and behavioral effects of OT has been reported. In the urgency to find treatments to syndromes as invalidating as autism, many clinical trials have been launched in which OT is administered to patients, including adolescents and children. However, the impact of OT on the developing brain and in particular on the embryonic and early postnatal maturation of OT neurons, has been only poorly investigated. In the present review we summarize available (although limited) literature on general features of ontogenetic transformation of the OT system, including determination, migration and differentiation of OT neurons. Next, we discuss trajectories of OT receptors (OTR) in the perinatal period. Furthermore, we provide evidence that early alterations, from birth, in the central OT system lead to severe neurodevelopmental diseases such as feeding deficit in infancy and severe defects in social behavior in adulthood, as described in Prader-Willi syndrome (PWS). Our review intends to propose a hypothesis about developmental dynamics of central OT pathways, which are essential for survival right after birth and for the acquisition of social skills later on. A better understanding of the embryonic and early postnatal maturation of the OT system may lead to better OT-based treatments in PWS or autism. PMID:25767437

  12. Predicting the Migration Rate of Dialkyl Organotins from PVC Pipe into Water

    EPA Science Inventory

    Organotins (OTs) are additives widely used as thermal and light stabilizers in polyvinyl chloride (PVC) plastics. OTs can leach into water flowing through PVC pipes. This work examines the leaching rates of two neurotoxic OTs, dimethyl tin (DMT) and dibutyl tin (DBT), from PVC pi...

  13. Aerosolized oxytocin increases cerebrospinal fluid oxytocin in rhesus macaques.

    PubMed

    Modi, Meera E; Connor-Stroud, Fawn; Landgraf, Rainer; Young, Larry J; Parr, Lisa A

    2014-07-01

    Intranasal (IN) administration is a widely used method for examining the effect of oxytocin (OT) on social behavior and cognition in healthy subjects and psychiatric populations. IN-OT in humans enhances trust, emotional perception, and empathetic behavior and is under investigation as a potential pharmacotherapy to enhance social functioning in a variety of neuropsychiatric disorders, including autism spectrum disorders (ASD). Nonhuman primates (NHP) are an important model for understanding the effect of OT on social cognition, its neural mechanisms, and the development of IN-OT as a pharmacotherapy for treating social deficits in humans. However, NHP and even some human populations, such as very young infants and children, cannot easily follow the detailed self-administration protocol used in the majority of human IN-OT studies. Therefore, we evaluated the efficacy of several OT-administration routes for elevating central OT concentrations in rhesus macaques. First, we examined the effect of IN and intravenous (IV) routes of OT administration on concentrations of OT and vasopressin (AVP) in plasma and lumbar CSF. Second, we examined these same measures in monkeys after an aerosolized (AE) OT delivery route. All three administration routes significantly increased plasma OT concentrations, but only the AE-OT route significantly increased concentrations of CSF OT. No route affected concentrations of AVP in plasma or CSF. This study confirms that the AE route is the most effective method for increasing central OT concentrations in monkeys, and may also be an effective route, alternative to IN, for administering OT to some human populations.

  14. Neonatal oxytocin alters subsequent estrogen receptor alpha protein expression and estrogen sensitivity in the female rat.

    PubMed

    Perry, Adam N; Paramadilok, Auratip; Cushing, Bruce S

    2009-12-14

    In most species, the effects of oxytocin (OT) on female reproductive behavior are dependent upon estrogen, which increases both OT and OT receptor expression. It is also becoming apparent that OT neurotransmission can influence estrogen signaling, especially during development, as neonatal OT manipulations in prairie voles alter ERalpha expression and estrogen-dependent behaviors. We tested the hypothesis that OT developmentally programs ERalpha expression and estrogen sensitivity in female Sprague-Dawley rats, a species previously used to establish the estrogen-dependence of OT signaling in adulthood. OT treatment for the first postnatal week significantly increased ERalpha-immunoreactivity in the ventromedial nucleus of the hypothalamus (VMH), but not in the medial preoptic area (MPOA). Conversely, neonatal OT antagonist (OTA) treatment significantly reduced ERalpha-immunoreactivity in the MPOA, but not in the VMH. Both treatments increased OT-immunoreactivity in the paraventricular nucleus of the hypothalamus (PVN) and reduced estrogen sensitivity, indicated by reduced sexual receptivity following chronic estradiol benzoate (EB) administration. Behavioral deficits in OTA-treated females were apparent during both paced and non-paced tests with 0.5 microg EB (but not 5.0 or 10.0 microg EB), whereas deficits in OT-treated females were only observed during the initial paced test with 0.5 and 5.0 microg EB (but not 10.0 microg EB). The current results demonstrate that OT can positively regulate ERalpha expression within the MPOA and VMH during development; however, endogenous OT selectively programs ERalpha expression within the MPOA. Thus, exogenous OT or OTA exposure during development may have long-term consequences on behavior through stable changes in ERalpha and OT expression.

  15. Oxytocin-induced elevation of ADP-ribosyl cyclase activity, cyclic ADP-ribose or Ca(2+) concentrations is involved in autoregulation of oxytocin secretion in the hypothalamus and posterior pituitary in male mice.

    PubMed

    Lopatina, Olga; Liu, Hong-Xiang; Amina, Sarwat; Hashii, Minako; Higashida, Haruhiro

    2010-01-01

    Locally released oxytocin (OT) activates OT receptors (2.1:OXY:1:OT:) in neighboring neurons in the hypothalamus and their terminals in the posterior pituitary, resulting in further OT release, best known in autoregulation occurring during labor or milk ejection in reproductive females. OT also plays a critical role in social behavior of non-reproductive females and even in males in mammals from rodents to humans. Social behavior is disrupted when elevation of free intracellular Ca(2+) concentration ([Ca(2+)](i)) and OT secretion are reduced in male and female CD38 knockout mice. Therefore, it is interesting to investigate whether ADP-ribosyl cyclase-dependent signaling is involved in OT-induced OT release for social recognition in males, independent from female reproduction, and to determine its molecular mechanism. Here, we report that ADP-ribosyl cyclase activity was increased by OT in crude membrane preparations of the hypothalamus and posterior pituitary in male mice, and that OT elicited an increase in [Ca(2+)](i) in the isolated terminals over a period of 5 min. The increases in cyclase and [Ca(2+)](i) were partially inhibited by nonspecific protein kinase inhibitors and a protein kinase C specific inhibitor, calphostin C. Subsequently, OT-induced OT release was also inhibited by calphostin C to levels inhibited by vasotocin, an OT receptor antagonist, and 8-bromo-cADP-ribose. These results demonstrate that OT receptors are functionally coupled to membrane-bound ADP-ribosyl cyclase and/or CD38 and suggest that cADPR-mediated intracellular calcium signaling is involved in autoregulation of OT release, which is sensitive to protein kinase C, in the hypothalamus and neurohypophysis in male mice.

  16. Predicting the Conditions for Homeotropic Anchoring of Liquid Crystals at a Soft Surface. 4-n-Pentyl-4'-cyanobiphenyl on Alkylsilane Self-Assembled Monolayers.

    PubMed

    Roscioni, Otello Maria; Muccioli, Luca; Zannoni, Claudio

    2017-04-05

    We have studied, using atomistic molecular dynamics simulations, the alignment of the nematic liquid-crystal 4-n-pentyl-4'-cyanobiphenyl (5CB) on self-assembled monolayers (SAMs) formed from octadecyl- and/or hexyltrichlorosilane (OTS and HTS) attached to glassy silica. We find a planar alignment on OTS at full coverage and an intermediate situation at partial OTS coverage because of the penetration of 5CB molecules into the monolayer, which also removes the tilt of the OTS SAM. Binary mixtures of HTS and OTS SAMs instead induce homeotropic (i.e., perpendicular) alignment. A comparison with the existing experimental literature is provided.

  17. CSF and blood oxytocin concentration changes following intranasal delivery in macaque.

    PubMed

    Dal Monte, Olga; Noble, Pamela L; Turchi, Janita; Cummins, Alex; Averbeck, Bruno B

    2014-01-01

    Oxytocin (OT) in the central nervous system (CNS) influences social cognition and behavior, making it a candidate for treating clinical disorders such as schizophrenia and autism. Intranasal administration has been proposed as a possible route of delivery to the CNS for molecules like OT. While intranasal administration of OT influences social cognition and behavior, it is not well established whether this is an effective means for delivering OT to CNS targets. We administered OT or its vehicle (saline) to 15 primates (Macaca mulatta), using either intranasal spray or a nebulizer, and measured OT concentration changes in the cerebral spinal fluid (CSF) and in blood. All subjects received both delivery methods and both drug conditions. Baseline samples of blood and CSF were taken immediately before drug administration. Blood was collected every 10 minutes after administration for 40 minutes and CSF was collected once post-delivery, at the 40 minutes time point. We found that intranasal administration of exogenous OT increased concentrations in both CSF and plasma compared to saline. Both delivery methods resulted in similar elevations of OT concentration in CSF, while the changes in plasma OT concentration were greater after nasal spray compared to nebulizer. In conclusion our study provides evidence that both nebulizer and nasal spray OT administration can elevate CSF OT levels.

  18. Oxytocin regulates changes of extracellular glutamate and GABA levels induced by methamphetamine in the mouse brain.

    PubMed

    Qi, Jia; Han, Wen-Yan; Yang, Jing-Yu; Wang, Li-Hui; Dong, Ying-Xu; Wang, Fang; Song, Ming; Wu, Chun-Fu

    2012-07-01

    Oxytocin (OT), a neurohypophyseal neuropeptide, affects adaptive processes of the central nervous system. In the present study, we investigated the effects of OT on extracellular levels of glutamate (Glu) and γ-aminobutyric acid (GABA) induced by methamphetamine (MAP) in the medial prefrontal cortex (mPFC) and dorsal hippocampus (DHC) of freely moving mice, using in vivo microdialysis coupled to high-performance liquid chromatography and fluorescence detection. The results showed that OT had no effect on basal Glu levels, but attenuated MAP-induced Glu increase in the mPFC and decrease in the DHC. OT increased the basal levels of extracellular GABA in mPFC and DHC of mice, and inhibited the MAP-induced GABA decrease in DHC. Western blot results indicated that OT significantly inhibited the increased glutamatergic receptor (NR1 subunit) levels in the PFC after acute MAP administration, whereas OT further enhanced the elevated levels of glutamatergic transporter (GLT1) induced by MAP in the hippocampus of mice. Atosiban, a selective inhibitor of OT receptor, antagonized the effects of OT. The results provided the first neurochemical evidence that OT, which exerted its action via its receptor, decreased Glu release induced by MAP, and attenuated the changes in glutamatergic neurotransmission partially via regulation of NR1 and GLT1 expression. OT-induced extracellular GABA increase also suggests that OT acts potentially as an inhibitory neuromodulator in mPFC and DHC of mice.

  19. An investigation of plasma and salivary oxytocin responses in breast- and formula-feeding mothers of infants.

    PubMed

    Grewen, Karen M; Davenport, Russell E; Light, Kathleen C

    2010-07-01

    Oxytocin (OT) is a peptide increasingly studied in relation to human social interactions, affiliation, and clinical disorders. Studies are constrained by use of invasive blood draws and would benefit from a reliable salivary OT assay. Our goals were to examine feasibility of salivary OT measurement, compare salivary to plasma OT responses in 12 breast- and 8 formula-feeding mothers, and assess the degree of correlation between plasma and salivary OT. Using a commercial EIA kit, we measured OT in 5 saliva and 7 plasma samples in a protocol designed to elicit changes in OT (Rest, Infant Interaction, Stress, Feeding). Breast-feeders had higher OT levels than formula-feeders across all conditions in plasma (+36%) and saliva (+23%). OT levels and ranges were similar in saliva and plasma, with slightly greater variance in saliva. Concurrently sampled plasma and salivary OT were correlated at end of Baseline Rest (r=+.59, p=.022) and Post-Stress Recovery (r=+.59, p=.025). These data suggest that salivary OT assay is feasible, and will be of value where plasma samples are not possible. Validation with larger samples is needed.

  20. Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation in Arabidopsis thaliana.

    PubMed

    Bailey, Mark; Srivastava, Anjil; Conti, Lucio; Nelis, Stuart; Zhang, Cunjin; Florance, Hannah; Love, Andrew; Milner, Joel; Napier, Richard; Grant, Murray; Sadanandom, Ari

    2016-01-01

    Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight into SUMO1/2-mediated regulation of SA signalling, we examined the status of SA-mediated defences in ots1/2 mutants. The ots1 ots2 double mutant displayed enhanced resistance to virulent Pseudomonas syringae and higher levels of SA compared with wild-type (WT) plants. Furthermore, ots1 ots2 mutants exhibited upregulated expression of the SA biosynthesis gene ICS1 in addition to enhanced SA-responsive ICS1 expression beyond that of WT. SA stimulated OTS1/2 degradation and promoted accumulation of SUMO1/2 conjugates. These results indicate that OTS1 and -2 act in a feedback loop in SA signalling and that de novo OTS1/2 synthesis works antagonistically to SA-promoted degradation, adjusting the abundance of OTS1/2 to moderate SA signalling. Accumulation of SUMO1/2 conjugates coincides with SA-promoted OTS degradation and may play a positive role in SA-mediated signalling in addition to its repressive roles reported elsewhere.

  1. Salivary Oxytocin Concentrations in Males following Intranasal Administration of Oxytocin: A Double-Blind, Cross-Over Study

    PubMed Central

    Daughters, Katie; Manstead, Antony S. R.; Hubble, Kelly; Rees, Aled; Thapar, Anita; van Goozen, Stephanie H. M.

    2015-01-01

    The use of intranasal oxytocin (OT) in research has become increasingly important over the past decade. Although researchers have acknowledged a need for further investigation of the physiological effects of intranasal administration, few studies have actually done so. In the present double-blind cross-over study we investigated the longevity of a single 24 IU dose of intranasal OT measured in saliva in 40 healthy adult males. Salivary OT concentrations were significantly higher in the OT condition, compared to placebo. This significant difference lasted until the end of testing, approximately 108 minutes after administration, and peaked at 30 minutes. Results showed significant individual differences in response to intranasal OT administration. To our knowledge this is the largest and first all-male within-subjects design study to demonstrate the impact of intranasal OT on salivary OT concentrations. The results are consistent with previous research in suggesting that salivary OT is a valid matrix for OT measurement. The results also suggest that the post-administration ‘wait-time’ prior to starting experimental tasks could be reduced to 30 minutes, from the 45 minutes typically used, thereby enabling testing during peak OT concentrations. Further research is needed to ascertain whether OT concentrations after intranasal administration follow similar patterns in females, and different age groups. PMID:26669935

  2. SCIRehab Project Series: The Occupational Therapy Taxonomy

    PubMed Central

    Ozelie, Rebecca; Sipple, Catherine; Foy, Teresa; Cantoni, Kara; Kellogg, Katherine; Lookingbill, Jennifer; Backus, Deborah; Gassaway, Julie

    2009-01-01

    Background/Objective: Lack of a classification system for occupational therapy (OT) rehabilitation interventions for traumatic spinal cord injury (SCI) rehabilitation in the United States makes conducting outcomes research difficult. This article describes an OT SCI rehabilitation taxonomy (system to categorize and classify treatments). Methods: OT clinicians and researchers from 6 SCI rehabilitation centers developed a taxonomy to describe details of each OT session. This effort is part of the SCIRehab study, which uses the practice-based evidence, observational research methodology to examine current treatment processes without changing existing practice. Results: The OT taxonomy consists of 26 OT activities (eg, training on activities of daily living, communication, home management skills, wheelchair mobility, bed mobility, transfers, balance, strengthening, stretching, equipment evaluation, and community reintegration). Time spent on each activity is documented along with therapeutic interventions used to facilitate the activity. Treatment descriptions are enhanced further with identification of assistance needs, patient direction of care, and family involvement, which help to describe and guide OT activity selection. The OT taxonomy documentation process includes all OT rehabilitation interventions for patients with SCI while maintaining efficiency in data collection. Conclusion: The electronic documentation system is being used at 6 centers for all OT sessions with 1,500 patients with acute traumatic SCI. It is the largest known attempt to document details of the comprehensive OT rehabilitation process for patients with SCI in the United States. PMID:19810630

  3. Differential sensitivity of intranuclear and systemic oxytocin release to central noradrenergic receptor stimulation during mid- and late gestation in rats.

    PubMed

    Lipschitz, David L; Crowley, William R; Bealer, Steven L

    2004-09-01

    A number of changes occur in the oxytocin (OT) system during gestation, such as increases in hypothalamic OT mRNA, increased neural lobe and systemic OT, and morphological and electrophysiological changes in OT-containing magnocellular neurons, suggestive of altered neuronal sensitivity, which may be mediated by ovarian steroids. Because central norepinephrine (NE) and histamine (HA) are potent stimulators of OT release during parturition and lactation, the present study investigated the effects of central noradrenergic and histaminergic receptor activation on systemic (NE, HA) and intranuclear (NE) OT release in pregnant rats and in ovariectomized rats treated with ovarian steroids. Plasma OT levels in late gestation were significantly higher compared with all other groups, and neither adrenergic nor histaminergic receptor blockade decreased these elevated levels. Furthermore, the alpha-adrenergic agonist phenylephrine, but not histamine, stimulated systemic OT release to a significantly greater extent in late gestation than in midpregnant, ovariectomized, or steroid-treated females. Although basal extracellular OT levels in the paraventricular nucleus, as measured with microdialysis, were unchanged during pregnancy or steroid treatment, noradrenergic receptor stimulation of intranuclear OT release was significantly elevated in midgestation females compared with all other groups. These studies indicate that sensitivity of intranuclear and systemic OT release to noradrenergic receptor activation differentially varies during the course of gestation.

  4. Regulation of 5alpha-reductase isoforms by oxytocin in the rat ventral prostate.

    PubMed

    Assinder, S J; Johnson, C; King, K; Nicholson, H D

    2004-12-01

    Oxytocin (OT) is present in the male reproductive tract, where it is known to modulate contractility, cell growth, and steroidogenesis. Little is known about how OT regulates these processes. This study describes the localization of OT receptor in the rat ventral prostate and investigates if OT regulates gene expression and/or activity of 5alpha-reductase isoforms I and II. The ventral prostates of adult male Wistar rats were collected following daily sc administration of saline (control), OT, a specific OT antagonist or both OT plus antagonist for 3 d. Expression of the OT receptor was identified in the ventral prostate by RT-PCR and Western blot, and confirmed to be a single active binding site by radioreceptor assay. Immunohistochemistry localized the receptor to the epithelium of prostatic acini and to the stromal tissue. Real-time RT-PCR determined that OT treatment significantly reduced expression of 5alpha-reductase I but significantly increased 5alpha-reductase II expression in the ventral prostate. Activity of both isoforms of 5alpha-reductase was significantly increased by OT, resulting in increased concentration of prostatic dihydrotestosterone. In conclusion, OT is involved in regulating conversion of testosterone to the biologically active dihydrotestosterone in the rat ventral prostate. It does so by differential regulation of 5alpha-reductase isoforms I and II.

  5. Enhancement of Organic Electroluminescent Intensity by Doping Hole-Property Polymers

    NASA Astrophysics Data System (ADS)

    Wang, Guangming; Wu, Haiming; Yuan, Chunwei; Wei, Yu

    1995-09-01

    Organic electroluminescent (EL) diodes with one-layer poly(3-octylthiophene) (P3OT) and P3OT doped with poly(N-vinylcarbazole) (PVK) (P3OT/PVK) as EL emitting layer sandwiched between indium/tin oxide (ITO) and aluminium electrodes have been fabricated by spin coating onto ITO respectively. The P3OT/PVK EL diode starts to emit red-orange light at 5 V in our experiment, the luminance and the current for the P3OT/PVK EL diode at bias voltage was compared with those for the only P3OT EL diode, indicating that the PVK dopant has power over the P3OT electroluminescence.

  6. Airloads investigation of an 0.030-scale model of the space shuttle vehicle 140A/B launch configuration (model 47-OTS) in the arc 11-foot unitary plan wind tunnel for Mach range 0.6 to 1.4 (IA14A), Volume 2

    NASA Technical Reports Server (NTRS)

    Gillins, R. L.

    1975-01-01

    Results of tests conducted on an 0.030-scale launch configuration model of the space shuttle vehicle 140A/B in the NASA/ARC 11-foot unitary plan wind tunnel are presented. Aerodynamic loads data were obtained at Mach numbers from 0.6 to 1.4. Surface pressure distributions were obtained simultaneously with six-component stability and control force data on the complete launch configuration. The configuration consisted of the orbiter, an external tank, two solid rocket boosters, and associated intercomponent attach hardware. Angles of attack and sideslip from -10 degrees to +10 degrees were investigated.

  7. Airloads investigation of an 0.030 scale model of the space shuttle vehicle 140A/B launch configuration (model 47-OTS) in the ARC 9 by 7 foot unitary plan wind tunnel for Mach 1.55 and 2.2 (iA14B), volume 1

    NASA Technical Reports Server (NTRS)

    Gillins, R. L.

    1975-01-01

    Tests were conducted in September, 1973 on a launch vehicle configuration consisting of a space shuttle orbiter, an external tank, two solid rocket boosters, and associated intercomponent attach hardware. Model variables included elevon, rudder, and speed brake deflections. Aerodynamic loads were measured and surface pressure distributions were obtained simultaneously with six-component stability and control force data on the complete configuration. Angles of attack and sideslip were also investigated.

  8. Results of a jet plume effects test on Rockwell International integrated space shuttle vehicle using a vehicle 5 configuration 0.02-scale model (88-OTS) in the 11 by 11 foot leg of the NASA/Ames Research Center unitary plan wind tunnel (IA19), volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1975-01-01

    Results are presented of jet plume effects test IA19 using a vehicle 5 configuration integrated space shuttle vehicle 0.02-scale model in the NASA/Ames Research Center 11 x 11-foot leg of the unitary plan wind tunnel. The jet plume power effects on the integrated vehicle static pressure distribution were determined along with elevon, main propulsion system nozzle, and solid rocket booster nozzle effectiveness and elevon hinge moments.

  9. Results of tests in the NASA/LaRC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/0A105), volume 1

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the 31-inch continuous Flow Hypersonic Wind Tunnel to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to RTLS abort separation. The model used was an 0.010-scale replica of the Space Shuttle Vehicle Configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the integrated Orbiter and external tank and the Orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and airleron deflections were also investigated.

  10. Results of tests OA26 and IA16 in the NASA/ARC 3.5-foot hypersonic wind tunnel on an 0.015-scale model (36-OTS) of the space shuttle configuration 140A/B to obtain pressures for venting analysis

    NASA Technical Reports Server (NTRS)

    Spangler, R. H.; Thornton, D. E.; Polek, T. E.

    1974-01-01

    Tests were conducted, from November 15 to December 4, 1973, to obtain surface pressure data on an 0.015-scale replica of the Space Shuttle Vehicle 4. Data were obtained at Mach numbers of 5.3, 7.4, and 10.3, to support the venting analysis for both launch and entry conditions. These tests were the final tests in a series covering a Mach number range from 0.6 to 10.3. The model was instrumented with pressure orifices in the vicinity of the cargo bay door hinge and parting lines, and on the side of the fuselage at the crew compartment, and below the orbital maneuvering system pods at the aft compartment. The model was tested at angles of attack and sideslip consistent with expected divergencies from the nominal trajectory.

  11. Use, trends, and impacts of nurse overtime in New York hospitals, 1995--2000

    NASA Astrophysics Data System (ADS)

    Berney, Barbara L.

    Over the last several years, nurses and their advocates have expressed concern about heavy use of overtime (OT) in hospitals and claimed that it undermines the quality of nursing care. Some hospital managers view OT as an important staffing and scheduling tool. This study examines trends in the use of nurse overtime and its effects on several nurse-sensitive patient outcomes. Previous studies have demonstrated a relationship between several patient outcomes and nurse staffing levels, but little is known about the impact of nurse overtime on patient outcomes. Using staffing and discharge data covering 1995 to 2000 from 160 acute general hospitals in New York State, this study examines hospital characteristics that are associated with the use of OT. These characteristics include size, location, RN unionization, hospital ownership and teaching status. The study examines trends in the use of OT. Finally, it uses multivariate regression to analyze the relationship between OT and the rates of six nurse-sensitive patient outcomes and mortality. Significant differences were observed in the use of OT by hospital ownership and by union status. Government hospitals used less OT than non-government hospitals. Non-union hospitals used slightly less OT than hospitals with unionized nurses. As total RN hours and straight (non-OT) hours per acute inpatient day increased, OT decreased. OT use as a percent of total inpatient RN hours increased significantly over the study period from 3.9% to 5.5%. Trends varied by hospital characteristic. This dissertation finds little evidence for an association between nurse OT and patient outcomes. One reason may be that the administrative data used track OT use and adverse events for whole years. Since OT use is episodic, data that examine the occurrence of adverse events during periods of heavy nurse overtime may be more sensitive to the impact of OT. That hospitals vary dramatically in their OT use and that some categories of hospitals (e

  12. Comparison Between GOES-12 Overshooting-Top Detections, WSR-88D Radar Reflectivity, and Severe Storm Reports

    NASA Technical Reports Server (NTRS)

    Dworak, Richard; Bedka, Kristopher; Brunner, Jason; Feltz, Wayne

    2012-01-01

    Studies have found that convective storms with overshooting-top (OT) signatures in weather satellite imagery are often associated with hazardous weather, such as heavy rainfall, tornadoes, damaging winds, and large hail. An objective satellite-based OT detection product has been developed using 11-micrometer infrared window (IRW) channel brightness temperatures (BTs) for the upcoming R series of the Geostationary Operational Environmental Satellite (GOES-R) Advanced Baseline Imager. In this study, this method is applied to GOES-12 IRW data and the OT detections are compared with radar data, severe storm reports, and severe weather warnings over the eastern United States. The goals of this study are to 1) improve forecaster understanding of satellite OT signatures relative to commonly available radar products, 2) assess OT detection product accuracy, and 3) evaluate the utility of an OT detection product for diagnosing hazardous convective storms. The coevolution of radar-derived products and satellite OT signatures indicates that an OT often corresponds with the highest radar echo top and reflectivity maximum aloft. Validation of OT detections relative to composite reflectivity indicates an algorithm false-alarm ratio of 16%, with OTs within the coldest IRW BT range (less than 200 K) being the most accurate. A significant IRW BT minimum typically present with an OT is more often associated with heavy precipitation than a region with a spatially uniform BT. Severe weather was often associated with OT detections during the warm season (April September) and over the southern United States. The severe weather to OT relationship increased by 15% when GOES operated in rapid-scan mode, showing the importance of high temporal resolution for observing and detecting rapidly evolving cloud-top features. Comparison of the earliest OT detection associated with a severe weather report showed that 75% of the cases occur before severe weather and that 42% of collocated severe

  13. Role of radiosensitivity and radioresistance genetic markers in hematological and cardiovascular disease in persons exposed after the Chornobyl accident.

    PubMed

    Minchenko, J M; Dyagil, I S; Dmytrenko, O O; Dmytrenko, I V; Shlaykhtychenko, T Y; Gavrylenko, T I; Biliy, D O; Khomenko, V I; Bebeshko, V G

    2013-01-01

    Meta roboty poljagala u vyvchenni vnesku imunogenetychnoi' komponenty v formuvannja postradiacijnyh efektiv u viddalenomu periodi pislja oprominennja na rivni imunnogo reaguvannja organizmu ljudyny, jak prognostychnogo kryteriju ocinky ryzyku realizacii' radiacijno-asocijovanoi' somatychnoi' patologii'. Ob’jektom doslidzhennja buly rekonvalescenty gostroi' promenevoi' hvoroby (GPH) I stupenja tjazhkosti i 88 pacijentiv z analogichnym radiacijnym anamnezom, ale z nepidtverdzhenym diagnozom GPH, 73 pacijenty, jaki majut' status uchasnykiv likvidacii' naslidkiv Chornobyl's'koi' katastrofy (ULNChK) z hronichnoju ishemichnoju hvoroboju sercja (HIHS), 65 pacijentiv – ULNChK bez HIHS, 120 neoprominenyh pacijentiv z HIHS. Pacijenty z onkogematologichnoju patologijeju – 256 osib. 500 praktychno zdorovyh osib – populjacijnyj kontrol'. Rezul'taty. Na osnovi vyvchennja spektru imunologichnyh, gematologichnyh ta molekuljarno-genetychnyh porushen' u spivvidnoshenni z imunogenetychnymy chynnykamy vyznacheni markery ryzyku realizacii' genetychnoi' shyl'nosti do onkogematologichnoi' ta sercevo-sudynnoi' patologii' u zaznachenyh kontyngentiv. Vysnovky. Otrymani dani svidchat' pro te, shho odnym iz mehanizmiv formuvannja radiacijno-asocijovanoi' mul'tyfaktorial'noi' patologii', je realizacija HLA-genetychnoi' shyl'nosti do zahvorjuvannja, jak na rivni rozladiv u gemopoezi ta imunopoezi, tak i na rivni zahvorjuvannja v cilomu, a nosijstvo v feno/genotypi markeriv radiochutlyvosti pidvyshhuje ryzyk realizacii' patologichnogo procesu v umovah oprominennja.

  14. Multiple myeloma among Chornobyl accident clean-up workers - state and perspectives of analytical study.

    PubMed

    Bazyka, D A; Gudzenko, N A; Dyagil, I S; Babkina, N G; Chumak, V V; Bakhanova, E V; Paramonov, V V; Romanenko, A Y

    2013-01-01

    Meta: proanalizuvaty zahvorjuvanist' na mnozhynnu mijelomu (MM) sered uchasnykiv likvidacii' naslidkiv Chornobyl's'koi' katastrofy (ULNA) ta pidgotuvaty informacijnu osnovu dlja podal'shogo analitychnogo doslidzhennja dozozalezhnyh ryzykiv cijei' patologii', vrahovujuchy i'i' potencijnu radiacijnu zumovlenist'. Materialy i metody. Dlja identyfikacii' vypadkiv MM u doslidzhuvanij kogorti ULNA chysel'nistju 152 520 osib cholovichoi' stati buv provedenyj linkidzh bazy danyh kogorty z informacijnym masyvom Nacional'nogo kancerrejestru Ukrai'ny. Rezul'taty. Za doslidzhuvanyj period (1987–2012 rr.) bulo identyfikovano 64 vypadky, 58 z jakyh buly vkljucheni do poperedn'ogo analizu zahvorjuvanosti, vrahovujuchy 10-richnyj lag-period. Za rezul'tatamy poperedn'ogo analizu zahvorjuvanist' na MM u doslidzhuvanij kogorti ULNA ne perevyshhuvala harakternyj dlja zagal'nogo naselennja riven' protjagom pershyh 21 rokiv pislja avarii'. Vysnovok. Rozrahunok standartyzovanogo spivvidnoshennja zahvorjuvanosti (SIR) za period 2008–2012 rr., tobto cherez 22–26 rokiv pislja avarii', prodemonstruvav dostovirne perevyshhennja rivnja sered ULNA porivnjano zi spontannym populjacijnym rivnem dlja naselennja Ukrai'ny cholovichoi' stati (SIR 1.61, 95% CI 1.01;2.21). Pislja provedennja nezalezhnoi' diagnostychnoi' ekspertyzy vsih identyfikovanyh vypadkiv, rekonstrukcii' otrymanoi' dozy zovnishn'ogo oprominennja na chervonyj kistkovyj mozok za metodom RADRUE budut' proanalizovani dozozalezhni ryzyky vynyknennja MM z urahuvannjam mozhlyvogo modyfikujuchogo vplyvu inshyh faktoriv.

  15. Ozone therapy ameliorates paraquat-induced lung injury in rats.

    PubMed

    Kaldirim, Umit; Uysal, Bulent; Yuksel, Ramazan; Macit, Enis; Eyi, Yusuf E; Toygar, Mehmet; Tuncer, Salim K; Ardic, Sukru; Arziman, Ibrahim; Aydin, Ibrahim; Oztas, Yesim; Karslioglu, Yildirim; Topal, Turgut

    2014-12-01

    Paraquat (PQ) overdose can cause acute lung injury and death. Ozone therapy (OT) was previously demonstrated to alleviate inflammation and necrosis in various pathologies. We therefore hypothesized that OT has ameliorative and preventive effects on PQ-induced lung damage due to anti-inflammatory and antioxidants properties. Sprague-Dawley rats (n = 24) were separated into three groups: sham, PQ, and PQ+OT groups. 15 mg/kg PQ was administered intraperitoneally in PQ and PQ+OT groups to induce experimental lung injury. One hour after PQ treatment, PQ+OT group was administered a single dose of ozone-oxygen mixture (1 mg/kg/day) by intraperitoneal route for four consecutive days. The animals were sacrificed on fifth day after PQ administration. Blood samples and lung tissues were collected to evaluate the inflammatory processes, antioxidant defense and pulmonary damage. Serum lactate dehydrogenase (LDH) and neopterin levels, tissue oxidative stress parameters, total TGF-β1 levels, and histological injury scores in PQ+OT group were significantly lower than PQ group (P<0.05, PQ vs. PQ+OT). Total antioxidant capacity in PQ+OT group was significantly higher than PQ group (P < 0.05, PQ+OT vs. PQ). These findings suggest that outcome in PQ-induced lung injury may be improved by using OT as an adjuvant therapy.

  16. A NOVEL GAMMAHERPESVIRUS IN NORTHERN FUR SEALS (CALLORHINUS URSINUS) IS CLOSELY RELATED TO THE CALIFORNIA SEA LION (ZALOPHUS CALIFORNIANUS) CARCINOMA-ASSOCIATED OTARINE HERPESVIRUS-1.

    PubMed

    Cortés-Hinojosa, Galaxia; Gulland, Frances M D; DeLong, Robert; Gelatt, Tom; Archer, Linda; Wellehan, James F X

    2016-01-01

    Otarine herpesvirus 1 (OtHV1) is strongly associated with California sea lion (CSL, Zalophus californianus) urogenital carcinoma, the most common cancer documented in marine mammals. In addition to CSL, OtHV1 has also been found in association with carcinoma in South American fur seals (Arctocephalus australis), demonstrating it can infect related species. Northern fur seals (NFS, Callorhinus ursinus) are sympatric with CSL, and copulation between these species has been observed; yet, there are no reports of urogenital carcinoma in NFS. We describe a new Otarine herpesvirus found in vaginal swabs from NFS, herein called OtHV4. Partial sequencing of the polymerase gene and the glycoprotein B gene revealed OtHV4 is closely related to OtHV1, with 95% homology in the region of polymerase sequenced, and phylogenetic analyses demonstrate that they are sister taxa. An OtHV4-specific hydrolysis probe quantitative PCR was developed and validated, and its use on vaginal swabs revealed 16 of 50 (32%) wild adult female NFS were positive for OtHV4. The identification of a virus highly similar to the carcinoma-associated OtHV1 in a sympatric species without carcinoma suggests that comparative genomics of OtHV1 and OtHV4 may identify candidate viral oncogenes.

  17. Intranasal oxytocin administration is reflected in human saliva.

    PubMed

    Weisman, Omri; Zagoory-Sharon, Orna; Feldman, Ruth

    2012-09-01

    Following the discovery that intranasal administration of neuropeptides can reach the central nervous system, a growing number of studies applied intranasal oxytocin (OT) paradigms to demonstrate the positive effects of OT on social and emotional processes. The three-step paradigm typically included: OT administration, a 45-min waiting period, and approximately 1-h period of active drug effects when experimental manipulations are applied. Yet, this schedule has not been put to systematic validation. Utilizing a double-blind placebo-control within-subject design, ten individuals were administered OT or placebo and salivary OT was measured ten times, at baseline and nine times over four consecutive hours. OT administration induced substantial increases in salivary OT across the entire period. OT rose dramatically 15 min after administration (from 6.9 pg/ml at baseline to 1265.4 pg/ml), reached plateau at 45-120 min (range=131.6 and 105.3 pg/ml), and did not return to baseline by 4h. Results contribute to discussion on brain-periphery coordination of OT and highlight the need for further research on the temporal dynamics and durations of OT administration effects.

  18. Effects of oxytocin on aggressive responding in healthy adult males

    PubMed Central

    Alcorn, Joseph L.; Green, Charles E.; Schmitz, Joy; Lane, Scott D.

    2015-01-01

    This study investigated the acute effects of oxytocin (OT) on human aggression using a well-established laboratory measure of state (reactive) aggression to test the hypothesis that OT would decrease the frequency of aggressive responding. In a within-subject design, 17 healthy male volunteers received placebo or 24 international units of intranasal OT. Aggression was measured via the Point Subtraction Aggression Paradigm at 30 min prior and 30, 60 and 90 min post-dose. Acute OT did not produce a significant main effect on aggressive behavior. OT attenuated the expected rise in diastolic blood pressure from morning to early afternoon observed under placebo, providing a possible indicator of biological activity. Examination of individual differences showed that aggressive responding following OT dosing (but not placebo) was positively correlated with psychometric measures of interpersonal manipulation and anger (Pearson’s r = 0.57), indicating that higher scores on these antisocial personality traits were related to increased aggressive behavior following OT administration. These preliminary results stand in contrast to previous work on the prosocial effects of OT and highlight the need for further understanding of individual differences in aggression following OT administration. Such individual differences may have implications for the therapeutic use of OT in individuals with psychiatric disorders and dysfunctional social behavior. PMID:26241153

  19. Indication of a species in an extinction vortex: The ocellated turkey on the Yucatan peninsula, Mexico

    NASA Astrophysics Data System (ADS)

    Kampichler, Christian; Calmé, Sophie; Weissenberger, Holger; Arriaga-Weiss, Stefan Louis

    2010-11-01

    The ocellated turkey Meleagris ocellata (OT) is a large, unmistakable endemic bird of the Yucatan peninsula. The species has suffered a considerable loss of distributional area as well as local abundance between 1980 and 2000 and is classified as endangered according to Mexican norms. We applied Classification Trees and Random Forests in order to determine the factors that most closely explain the observed patterns of distribution and abundance loss, and to develop hypotheses that may guide measures for the protection of the OT. Among the most important predictors of change were variables corresponding to aspects of forest cover and variables on human population and small settlements. OT abundance in 1980, however, was by far the most important predictor for OT abundance change. This is an indication that the OT dynamics are governed by internal rather than by external factors. Medium and low abundances in 1980 inevitably led to a further decrease during the following years, which gives rise to the conclusion that the OT might find itself in an extinction vortex. We suggest the following hypothetical scenario for OT decline: migrant people from other Mexican states colonise forested regions in Yucatan; they establish small settlements; bushmeat hunting is important for their survival; the naïve OT is easy prey; hunting—together with beginning deforestation—reaches a certain level, and local OT abundance falls below a critical threshold; OT continues declining regardless of current social and environmental changes except where there is total protection of both the species and its habitat.

  20. Orthodontic treatment for disabled children: a survey of parents’ attitudes and overall satisfaction

    PubMed Central

    2014-01-01

    Background Many patients with disability require orthodontic treatment (OT) to achieve adequate oral function and aesthetic appearance. The cooperation of disabled patients and of their parents is central to the success of OT, as treatment can involve ethical dilemmas. The aim of this study was to analyze the motivation, expectations and overall satisfaction with OT among parents of patients with disabilities. Methods The parents of 60 disabled Spanish children with physical, mental and/or sensory impairment undergoing OT were surveyed on attitudes to OT and level of satisfaction with the outcomes. The survey consisted of 23 questions in 4 sections: attitude and adaptation, benefits, adverse effects, and level of satisfaction after completion of OT. A control group formed of the parents of 60 healthy children undergoing OT at the same institution were also surveyed. Results Parents of disabled children undergoing OT showed a high level of motivation and they are willing to collaborate in oral hygiene procedures. Adaptation to the removable appliances was poorer in disabled children but adaptation to fixed appliances was excellent. OT can provide a marked improvement in quality of life, social relationships and oral functionality in disabled children. Conclusions Among parents of disabled children undergoing OT, the perceived level of overall satisfaction was very high and expectations were often exceeded. PMID:25096027

  1. Optimization of the radiological protection of patients undergoing digital radiography.

    PubMed

    Zhang, Menglong; Chu, Cunkun

    2012-02-01

    Because of a much higher dynamic range of flat panel detectors, patient dose can vary without change of image quality being perceived by radiologists. This condition makes optimization (OT) of radiation protection undergoing digital radiography (DR) more complex, while a chance to reduced patient dose also exists. In this study, we evaluated the difference of patient radiation and image rejection before and after OT to identify if it is necessary to carry out an OT procedure in a routine task with DR. The study consisted of a measurement of the dose area product (DAP) and entrance surface dose (ESD) received by a reference group of patients for eight common radiographic procedures using the DR system before and after OT. Meanwhile image rejection data during two 2-month periods were collected and sorted according to reason. For every radiographic procedure, t tests showed significant difference in average ESD and DAP before and after OT (p < 0.005). The ESDs from most examinations before OT were three times higher than that after OT. For DAPs, the difference is more significant. Image rejection rate after OT is significantly lower than that before OT (χ (2) = 36.5, p < 0.005). The substantial reductions of dose after OT resulted from appropriate mAs and exposure field. For DR patient dose, less than recommended diagnostic reference level can meet quality criteria and clinic diagnosis.

  2. Salivary oxytocin in adolescents with conduct problems and callous-unemotional traits.

    PubMed

    Levy, Tomer; Bloch, Yuval; Bar-Maisels, Meytal; Gat-Yablonski, Galia; Djalovski, Amir; Borodkin, Katy; Apter, Alan

    2015-12-01

    Callous-unemotional (CU) traits correlate with the severity and prognosis of conduct disorder in youth. The neuropeptide oxytocin (OT) has been linked to prosocial behaviors, including empathy and collaboration with others. This study discusses a possible role for OT in the biology of delinquent behavior. We hypothesized that in delinquent youth OT secretion will correlate with the severity of conduct problems and specifically with the level of CU traits. The study group included 67 male adolescents (mean age 16.2 years) undergoing residential treatment, previously assessed by an open clinical interview and history for the psychiatric diagnosis. Staff based Inventory of Callous-Unemotional traits for psychopathy and Strength and Difficulties Questionnaire were administered, and patients' medical and social personal files were systematically coded for previous history of antisocial acts using the Brown-Goodwin Questionnaire. Salivary OT was assayed by ELISA. Salivary OT levels were inversely correlated with conduct problems severity on Strength and Difficulties Questionnaire (r = -0.27; p ≤ 0.01). Recorded history of antisocial acts did not correlate with current OT levels. Odds ratio (OR) for significant CU traits among subjects with conduct problems was increased in low-OT (OR = 14, p ≤ 0.05) but not in high-OT subjects (OR = 6, p ≥ 0.05). Children with conduct problems and low levels of salivary OT are at risk for significant CU traits. These results suggest a possible role for salivary OT as a biomarker for CU traits and conduct problems severity.

  3. Oxytocin and Estrogen Receptor β in the Brain: An Overview

    PubMed Central

    Acevedo-Rodriguez, Alexandra; Mani, Shaila K.; Handa, Robert J.

    2015-01-01

    Oxytocin (OT) is a neuropeptide synthesized primarily by neurons of the paraventricular and supraoptic nuclei of the hypothalamus. These neurons have axons that project into the posterior pituitary and release OT into the bloodstream to promote labor and lactation; however, OT neurons also project to other brain areas where it plays a role in numerous brain functions. OT binds to the widely expressed OT receptor (OTR), and, in doing so, it regulates homeostatic processes, social recognition, and fear conditioning. In addition to these functions, OT decreases neuroendocrine stress signaling and anxiety-related and depression-like behaviors. Steroid hormones differentially modulate stress responses and alter OTR expression. In particular, estrogen receptor β activation has been found to both reduce anxiety-related behaviors and increase OT peptide transcription, suggesting a role for OT in this estrogen receptor β-mediated anxiolytic effect. Further research is needed to identify modulators of OT signaling and the pathways utilized and to elucidate molecular mechanisms controlling OT expression to allow better therapeutic manipulations of this system in patient populations. PMID:26528239

  4. Oxytocin Levels are Lower in Premenopausal Women with Type 1 Diabetes Mellitus Compared to Matched Controls

    PubMed Central

    Kujath, Amber S.; Quinn, Lauretta; Elliott, Mary E.; Varady, Krista A.; LeCaire, Tamara J.; Carter, C. Sue; Danielson, Kirstie K.

    2014-01-01

    Background Oxytocin (OT), a hormone most commonly associated with parturition and lactation, may have additional roles in diabetes complications. We determined OT levels in premenopausal women with type 1 diabetes mellitus (T1DM) compared to nondiabetic controls; and examined associations of OT with health behaviors, clinical factors, biomarkers, kidney function, and bone health. Lower OT was hypothesized for T1DM. Methods Cross-sectional study of premenopausal women with T1DM (n=88) from the Wisconsin Diabetes Registry Study, a population-based cohort of incident T1DM cases, and matched nondiabetic controls (n=74). Results Women with T1DM had lower OT levels than controls adjusting for caffeine and alcohol use (p=0.03). Health behaviors associated with OT differed between women with and without T1DM: OT was negatively associated with hormonal contraceptive use (quantified as lifetime contraceptive estrogen exposure) in women with T1DM (p=0.003) while positively related to hormonal contraceptive use (quantified as never/former/current) in controls (p<0.001). OT had a positive association with adiposity (waist-to-hip ratio and leptin) in women with T1DM and a negative relationship with adiposity (weight gain) in controls. In T1DM only, OT was positively associated with caffeine intake (p=0.01) and negatively associated with alcohol use (p=0.01). OT was not related to glycemic control, kidney function, or bone health in T1DM. Conclusions OT levels are lower in women with T1DM than matched controls. OT also has opposing associations with hormonal contraceptives and adiposity in women with and without T1DM. Research is needed to determine if the altered OT milieu in T1DM is associated with other health outcomes. PMID:25044726

  5. Medial nucleus tractus solitarius oxytocin receptor signaling and food intake control: the role of gastrointestinal satiation signal processing

    PubMed Central

    Alhadeff, Amber L.; Grill, Harvey J.

    2015-01-01

    Central oxytocin (OT) administration reduces food intake and its effects are mediated, in part, by hindbrain oxytocin receptor (OT-R) signaling. The neural substrate and mechanisms mediating the intake inhibitory effects of hindbrain OT-R signaling are undefined. We examined the hypothesis that hindbrain OT-R-mediated feeding inhibition results from an interaction between medial nucleus tractus solitarius (mNTS) OT-R signaling and the processing of gastrointestinal (GI) satiation signals by neurons of the mNTS. Here, we demonstrated that mNTS or fourth ventricle (4V) microinjections of OT in rats reduced chow intake in a dose-dependent manner. To examine whether the intake suppressive effects of mNTS OT-R signaling is mediated by GI signal processing, rats were injected with OT to the 4V (1 μg) or mNTS (0.3 μg), followed by self-ingestion of a nutrient preload, where either treatment was designed to be without effect on chow intake. Results showed that the combination of mNTS OT-R signaling and GI signaling processing by preload ingestion reduced chow intake significantly and to a greater extent than either stimulus alone. Using enzyme immunoassay, endogenous OT content in mNTS-enriched dorsal vagal complex (DVC) in response to ingestion of nutrient preload was measured. Results revealed that preload ingestion significantly elevated endogenous DVC OT content. Taken together, these findings provide evidence that mNTS neurons are a site of action for hindbrain OT-R signaling in food intake control and that the intake inhibitory effects of hindbrain mNTS OT-R signaling are mediated by interactions with GI satiation signal processing by mNTS neurons. PMID:25740340

  6. Medial nucleus tractus solitarius oxytocin receptor signaling and food intake control: the role of gastrointestinal satiation signal processing.

    PubMed

    Ong, Zhi Yi; Alhadeff, Amber L; Grill, Harvey J

    2015-05-01

    Central oxytocin (OT) administration reduces food intake and its effects are mediated, in part, by hindbrain oxytocin receptor (OT-R) signaling. The neural substrate and mechanisms mediating the intake inhibitory effects of hindbrain OT-R signaling are undefined. We examined the hypothesis that hindbrain OT-R-mediated feeding inhibition results from an interaction between medial nucleus tractus solitarius (mNTS) OT-R signaling and the processing of gastrointestinal (GI) satiation signals by neurons of the mNTS. Here, we demonstrated that mNTS or fourth ventricle (4V) microinjections of OT in rats reduced chow intake in a dose-dependent manner. To examine whether the intake suppressive effects of mNTS OT-R signaling is mediated by GI signal processing, rats were injected with OT to the 4V (1 μg) or mNTS (0.3 μg), followed by self-ingestion of a nutrient preload, where either treatment was designed to be without effect on chow intake. Results showed that the combination of mNTS OT-R signaling and GI signaling processing by preload ingestion reduced chow intake significantly and to a greater extent than either stimulus alone. Using enzyme immunoassay, endogenous OT content in mNTS-enriched dorsal vagal complex (DVC) in response to ingestion of nutrient preload was measured. Results revealed that preload ingestion significantly elevated endogenous DVC OT content. Taken together, these findings provide evidence that mNTS neurons are a site of action for hindbrain OT-R signaling in food intake control and that the intake inhibitory effects of hindbrain mNTS OT-R signaling are mediated by interactions with GI satiation signal processing by mNTS neurons.

  7. [Therapeutic effects of oxytocin in autism: Current status of the research].

    PubMed

    Gauthier, C; Doyen, C; Amado, I; Lôo, H; Gaillard, R

    2016-02-01

    The neuropeptide oxytocin (OT) is an evolutionary highly conserved molecule that plays a part in the regulation of complex social cognition and behaviours. From a pathophysiological point of view, several studies have evidenced dysfunctions of the oxytocinergic system in autism spectrum disorders (ASD): a lowering of plasma OT and genetic or epigenetic anomalies of the OT receptor. Therefore, some authors have hypothesized that an abnormality in the OT neurotransmission may account for several features of autism and that a treatment restoring a normal OT pathway functioning could improve social abilities. OT administration has thus been used in clinical trials, especially in groups of subjects suffering from autism. Some studies found that OT decreased repetitive behaviours, enhanced emotional understanding of speech intonation, improved performance of the Reading the Mind in the Eyes Test and reinforced cooperation. Nevertheless, the findings of the OT administration studies on clinical samples show great diversity. The context, the personality and childhood experiences of the subject could be moderators influencing the effect of exogenous OT. Besides, three mechanisms could play a part in the action of OT on ASD social symptoms: anxiety reduction (with a lowering in the hypothalamic-pituitary-adrenal axis responsiveness and in the amygdale reactivity to social stimuli), increased affiliative motivation (involving the dopaminergic pathway and several regions of the social brain) and enhanced perceptual selectivity and social stimuli salience. To conclude, OT could be a promising molecule used as a treatment to promote social behaviours, helping individuals with ASD to develop new relationships. OT could be administered during a cognitive-behavioural therapy to reinforce the efficacy of such procedures. More studies are needed, on larger samples, to investigate the safety and efficacy of OT administration and to specify optimal dosages and characteristics of

  8. Convective overshooting top detection with MSG SEVIRI, Himawari-8 AHI, and CloudSat CPR data

    NASA Astrophysics Data System (ADS)

    Im, J.; Kim, M.; Park, S.

    2015-12-01

    Overshooting Tops (OTs) are the clouds that penetrate into the tropopause and grow to the bottom of stratosphere at the top layer of cumulonimbus with very strong updraft. Severe weather conditions such as ground lightning, large hail, strong winds, and heavy rainfall can cause in the cumulonimbus clouds with OTs, with turbulence and lightning occurring very frequently in the area near OTs. In terms of aviation operations, OTs are a very important risk factor. According to Federal Aviation Administration, 509 cases of 4,326 weather-related events from 1992 to 2001were caused by turbulences. The detection of OTs is important to predict the degree and location of severe weather conditions such as turbulence, lightning, and thunderstorms. There are two methods widely used to detect OTs with multispectral images. One is the Water Vapor-InfraRed window channel Brightness Temperature Difference (WV-IRW BTD), which uses the differences in brightness temperatures at an infrared channel (about 11 ㎛). The other approach is the InfraRed Window texture (IRW-texture) method, which is based on the characteristics of OTs that appear a pixel group with low temperatures. The typical IRW-texture algorithm uses simple thresholds to detect OTs, whereas this research proposes an advanced approach based on machine learning techniques such as decision trees, random forest (RF), and support vector machines (SVM) with various variables from geostationary satellite data such as MSG SEVIRI (over Africa) and Himawari AHI (over East Asia) so as to improve the detection of OTs. OT and non-OT samples (e.g. other types of clouds such as stratus and cirrus) were extracted using the CloudSat cloud profiling radar (CPR) and SEVIRI (and Himawari) imagery. Results show that RF produced the best performance in detection of OTs yielding an overall accuracy of 98.33% and a false alarm rate of 9.01%. The user's accuracies of OT and non-OT were similar, whereas the producer's accuracy of non-OT was

  9. Oxytocin, but not vassopressin, modulates nociceptive responses in dorsal horn neurons.

    PubMed

    Rojas-Piloni, Gerardo; Gerardo, Rojas-Piloni; Mejía-Rodríguez, Rosalinda; Rosalinda, Mejía-Rodríguez; Martínez-Lorenzana, Guadalupe; Guadalupe, Martínez-Lorenzana; Condés-Lara, Miguel; Miguel, Condés-Lara

    2010-05-26

    Oxytocin (OT) and vasopressin (VP) are synthesized and secreted by the paraventricular hypothalamic nucleus (PVN), and both peptides have been implicated in the pain modulatory system. In the spinal cord, activation of OT-containing axons modulates nociceptive neuronal responses in dorsal horn neurons; however, it is not known whether the direct VPergic descending projection participates. Here, we show that both PVN electrical stimulation and topical application of OT in the vicinity of identified and recorded dorsal horn WDR selectively inhibit Adelta and C-fiber responses. In contrast, the topical administration of VP on the same neurons did not affect the nociceptive responses. In addition, the reduction in nociceptive responses caused by PVN stimulation or OT administration was blocked with a selective OT antagonist. The results suggest that the VP descending projection does not modulate the antinociceptive effects mediated by the PVN on dorsal horn neurons; instead, it is the hypothalamic-spinal OT projection that regulates nociceptive information.

  10. Do delivery routes of intranasally administered oxytocin account for observed effects on social cognition and behavior? A two-level model.

    PubMed

    Quintana, Daniel S; Alvares, Gail A; Hickie, Ian B; Guastella, Adam J

    2015-02-01

    Accumulating evidence demonstrates the important role of oxytocin (OT) in the modulation of social cognition and behavior. This has led many to suggest that the intranasal administration of OT may benefit psychiatric disorders characterized by social dysfunction, such as autism spectrum disorders and schizophrenia. Here, we review nasal anatomy and OT pathways to central and peripheral destinations, along with the impact of OT delivery to these destinations on social behavior and cognition. The primary goal of this review is to describe how these identified pathways may contribute to mechanisms of OT action on social cognition and behavior (that is, modulation of social information processing, anxiolytic effects, increases in approach-behaviors). We propose a two-level model involving three pathways to account for responses observed in both social cognition and behavior after intranasal OT administration and suggest avenues for future research to advance this research field.

  11. The Effects of Intranasal Oxytocin Administration on Sensitive Caregiving in Mothers with Postnatal Depression.

    PubMed

    Mah, Beth L; Van Ijzendoorn, Marinus H; Out, Dorothee; Smith, Roger; Bakermans-Kranenburg, Marian J

    2017-04-01

    Postnatal depression (PND) is common and negatively affects the mother-infant relationship; oxytocin (OT) has been found to have positive effects on parenting, although psychiatric disorders may reduce these effects. Thus, we explored the role of OT in mothers diagnosed with PND. A within-subject, randomized controlled double-blind design was used to test the effects of nasal administration of OT or placebo on sensitive caregiving. The outcome measures were perceptual and caregiving responses to prerecorded cry sounds, as well as observed maternal sensitivity. We found that in the OT condition mothers with PND were more likely to rate an infant cry as more urgent and they were more likely to indicate they would chose a harsh caregiving strategy in response. There was no effect of OT on maternal sensitive interaction with their own baby. Further research is required prior to consideration of OT administration in depressed mothers of infants.

  12. Problems with measuring peripheral oxytocin: can the data on oxytocin and human behavior be trusted?

    PubMed

    McCullough, Michael E; Churchland, Patricia Smith; Mendez, Armando J

    2013-09-01

    Research on the neurobiological and behavioral effects of oxytocin (OT), as well as on its possible therapeutic applications, has intensified in the past decade. Accurate determination of peripheral OT levels is essential to reach meaningful conclusions and to motivate, support and inform clinical interventions. Different, but concordant, methods for measuring plasma OT have been developed over the past four decades, but since 2004 several commercially available methods have been favored in research with humans. Evaluation of these methods reveals that they lack reliability when used on unextracted samples of human fluids, and that they tag molecules in addition to OT, yielding estimates that are wildly discrepant with an extensive body of earlier findings that were obtained using methods that are well validated, but more laborious. An accurate, specific, and readily available method for measuring OT that can be adopted as the standard in the field is urgently needed for advances in our understanding of OT's roles in cognition and behavior.

  13. Oxytocin system dysfunction as a common mechanism underlying metabolic syndrome and psychiatric symptoms in schizophrenia and bipolar disorders.

    PubMed

    Quintana, Daniel S; Dieset, Ingrid; Elvsåshagen, Torbjørn; Westlye, Lars T; Andreassen, Ole A

    2017-01-01

    There is growing interest in using intranasal oxytocin (OT) to treat social dysfunction in schizophrenia and bipolar disorders (i.e., psychotic disorders). While OT treatment results have been mixed, emerging evidence suggests that OT system dysfunction may also play a role in the etiology of metabolic syndrome (MetS), which appears in one-third of individuals with psychotic disorders and associated with increased mortality. Here we examine the evidence for a potential role of the OT system in the shared risk for MetS and psychotic disorders, and its prospects for ameliorating MetS. Using several studies to demonstrate the overlapping neurobiological profiles of metabolic risk factors and psychiatric symptoms, we show that OT system dysfunction may be one common mechanism underlying MetS and psychotic disorders. Given the critical need to better understand metabolic dysregulation in these disorders, future OT trials assessing behavioural and cognitive outcomes should additionally include metabolic risk factor parameters.

  14. Fuel and Fuel System Materials Compatibility Test Program for A JP-8+100 Fuel Additive. Volume 1: Thermal Stability Additive Package BetzDearborn Spec Aid(Registered) 8Q462

    DTIC Science & Technology

    2001-10-01

    W W W II.G.2 Gasket “O” Ring MIL-R-25988 Fluorosi l icone OT W W II.G.3 Gasket “O” Ring MIL-R-83485 Fluorosi l icone...O W W II.G.7 Gasket “O” Ring MIL-R-25988 Fluorosi l icone OT OT W ¹ Tested as a coating (tape adhesion and pencil) 73

  15. Oxytocin differentially alters resting state functional connectivity between amygdala subregions and emotional control networks: Inverse correlation with depressive traits.

    PubMed

    Eckstein, Monika; Markett, Sebastian; Kendrick, Keith M; Ditzen, Beate; Liu, Fang; Hurlemann, Rene; Becker, Benjamin

    2017-04-01

    The hypothalamic neuropeptide oxytocin (OT) has received increasing attention for its role in modulating social-emotional processes across species. Previous studies on using intranasal-OT in humans point to a crucial engagement of the amygdala in the observed neuromodulatory effects of OT under task and rest conditions. However, the amygdala is not a single homogenous structure, but rather a set of structurally and functionally heterogeneous nuclei that show distinct patterns of connectivity with limbic and frontal emotion-processing regions. To determine potential differential effects of OT on functional connectivity of the amygdala subregions, 79 male participants underwent resting-state fMRI following randomized intranasal-OT or placebo administration. In line with previous studies OT increased the connectivity of the total amygdala with dorso-medial prefrontal regions engaged in emotion regulation. In addition, OT enhanced coupling of the total amygdala with cerebellar regions. Importantly, OT differentially altered the connectivity of amygdala subregions with distinct up-stream cortical nodes, particularly prefrontal/parietal, and cerebellar down-stream regions. OT-induced increased connectivity with cerebellar regions were largely driven by effects on the centromedial and basolateral subregions, whereas increased connectivity with prefrontal regions were largely mediated by right superficial and basolateral subregions. OT decreased connectivity of the centromedial subregions with core hubs of the emotional face processing network in temporal, occipital and parietal regions. Preliminary findings suggest that effects on the superficial amygdala-prefrontal pathway were inversely associated with levels of subclinical depression, possibly indicating that OT modulation may be blunted in the context of increased pathological load. Together, the present findings suggest a subregional-specific modulatory role of OT on amygdala-centered emotion processing networks in

  16. Successful thalamic deep brain stimulation for orthostatic tremor.

    PubMed

    Guridi, Jorge; Rodriguez-Oroz, Maria C; Arbizu, Javier; Alegre, Manuel; Prieto, Elena; Landecho, Ignacio; Manrique, Miguel; Artieda, Julio; Obeso, Jose A

    2008-10-15

    We report a patient with severe orthostatic tremor (OT) unresponsive to pharmacological treatments that was successfully controlled with thalamic (Vim, ventralis intermedius nucleus) deep brain stimulation (DBS) over a 4-year period. Cortical activity associated with the OT revealed by EEG back-averaging and fluoro-deoxi-glucose PET were also suppressed in parallel with tremor arrest. This case suggests that Vim-DBS may be a useful therapeutic approach for patients highly disabled by OT.

  17. Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment.

    PubMed

    Quintana, D S; Westlye, L T; Rustan, Ø G; Tesli, N; Poppy, C L; Smevik, H; Tesli, M; Røine, M; Mahmoud, R A; Smerud, K T; Djupesland, P G; Andreassen, O A

    2015-07-14

    Despite the promise of intranasal oxytocin (OT) for modulating social behavior, recent work has provided mixed results. This may relate to suboptimal drug deposition achieved with conventional nasal sprays, inter-individual differences in nasal physiology and a poor understanding of how intranasal OT is delivered to the brain in humans. Delivering OT using a novel 'Breath Powered' nasal device previously shown to enhance deposition in intranasal sites targeted for nose-to-brain transport, we evaluated dose-dependent effects on social cognition, compared response with intravenous (IV) administration of OT, and assessed nasal cavity dimensions using acoustic rhinometry. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults completing four single-dose treatments (intranasal 8 IU (international units) or 24 IU OT, 1 IU OT IV and placebo). The primary outcome was social cognition measured by emotional ratings of facial images. Secondary outcomes included the pharmacokinetics of OT, vasopressin and cortisol in blood and the association between nasal cavity dimensions and emotional ratings. Despite the fact that all the treatments produced similar plasma OT increases compared with placebo, there was a main effect of treatment on anger ratings of emotionally ambiguous faces. Pairwise comparisons revealed decreased ratings after 8 IU OT in comparison to both placebo and 24 IU OT. In addition, there was an inverse relationship between nasal valve dimensions and anger ratings of ambiguous faces after 8-IU OT treatment. These findings provide support for a direct nose-to-brain effect, independent of blood absorption, of low-dose OT delivered from a Breath Powered device.

  18. Effects of water concentration in the coating solution on the wall relaxation rate of octadecyltrichlorosilane coated rubidium vapor cells

    SciTech Connect

    Zhang, Guiying; Wei, Lihua; Wang, Meiling; Zhao, Kaifeng

    2015-01-28

    High quality anti-relaxation surface coatings for atomic vapor cells are essential for the preservation of atomic spin coherence and the enhancement of measurement sensitivity. In this paper, we studied the effects of water concentration in octadecyltrichlorosilane (OTS) coating solution on the relaxation rate and its reproducibility of OTS coated Rubidium vapor cells. We found that appropriate water concentration can improve the anti-relaxation performance of OTS coated cells.

  19. Yttrium Siloxide Complexes Bearing Terminal Methyl Ligands: Molecular Models for Ln-CH3 Terminated Silica Surfaces.

    PubMed

    Dettenrieder, Nicole; Dietrich, H Martin; Maichle-Mössmer, Cäcilia; Anwander, Reiner

    2016-09-05

    Surface organometallic chemistry (SOMC) on silica materials is a prominent approach for the generation of highly active heterogenized polymerization catalysts. Despite advanced methods of characterization, the elucidation of the catalytically active surface species remains a challenging task. Alkylated rare-earth metal siloxide complexes can be regarded as molecular models of respective covalently bonded alkylated surface species, primarily used for 1,3-diene polymerization. Here, we performed both salt metathesis reactions of [Y(MMe4 )3 ] (M = Al, Ga) with [K{OSi(OtBu)3 }] and alkylation reactions of [Y{OSi(OtBu)3 }3 ]2 with AlMe3 . The obtained complexes [Y(CH3 )[(AlMe2 ){OSi(OtBu)3 }2 ](AlMe4 )]2 , [Y(CH3 )[(AlMe2 ){OSi(OtBu)3 }2 ]-{OSi(OtBu)3 }], [Y{OSi(OtBu)3 }3 (μ-Me)Y(μ-Me)2 Y{OSi(OtBu)3 }2 (AlMe4 )], and [Y(CH3 )(GaMe4 ){OSi(OtBu)3 }]2 represent rare examples of organoyttrium species with terminal methyl groups. The formation and purity of the mixed methyl/siloxy yttrium complexes could be enhanced by treating [Y(MMe4 )3 ] with [K(MMe2 ){OSi(OtBu)3 }2 ]n (M=Al: n=2; M=Ga: n=∞). Complexes [K(MMe2 ){OSi(OtBu)3 }2 ]n were obtained by addition of [K{OSi(OtBu)3 }] to [Me2 M{OSi(OtBu)3 }]2 . Deeper insight into the fluxional behavior of the mixed methyl/siloxy yttrium complexes in solution was gained by (1) H and (13) C NMR spectroscopic studies at variable temperature and (1) H-(89) Y HSQC NMR spectroscopy.

  20. Effect of environmental pollutants on oxytocin synthesis and secretion from corpus luteum and on contractions of uterus from pregnant cows.

    PubMed

    Mlynarczuk, Jaroslaw; Wrobel, Michal H; Kotwica, Jan

    2010-09-15

    Chloro-organic compounds are persistent environmental pollutants and affect many reproductive processes. Oxytocin (OT) synthesized in luteal cells is a local regulator of ovarian activity and uterine contractions. Therefore the effect of xenobiotics on the OT prohormone synthesis, secretion of OT and progesterone (P4) from luteal cells and on myometrial contractions during early pregnancy in cows was investigated. Luteal cells and myometrial strips from a cow at early pregnancy were treated with polychlorinated biphenyl 77 (PCB 77), dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE) and hexachlorocyclohexane (HCH) (1 or 10 ng/ml). The mRNA expression of neurophysin-I/oxytocin (NP-I/OT) and peptidyl-glycine-alpha-amidating mono-oxygenase (PGA) and concentration of OT and P4 were determined by RT-PCR and EIA, respectively. Moreover, the effect of xenobiotics given with P4 (12 ng/ml) on the basal and OT (10(-7)M) stimulated contractions of myometrial strips was studied. Xenobiotics increased (P<0.05) OT secretion but DDE only stimulated P4 secretion. The ratio of P4 to OT in culture medium was decreased by all xenobiotics during 9-12 weeks of pregnancy. All xenobiotics, except HCH, increased (P<0.05) mRNA expression of NP-I/OT during all stages of pregnancy and all treatments decreased (P<0.05) expression of mRNA for PGA during 9-12 weeks of pregnancy. Myometrial strips were relaxed (P<0.01) after pre-incubation with P4, while each of the xenobiotics jointly with P4 increased (P<0.01) myometrial contractions. In conclusion, the xenobiotics used increased both expression of mRNA for genes involved in OT synthesis and secretion of OT from luteal cells. This decreases the ratio of P4 to OT and presumably, in this manner, the chloro-organic compounds can influence uterine contractions and enhance risk of abortions in pregnant females.

  1. Neural Basis of Empathy and Its Dysfunction in Autism Spectrum Disorders (ASD)

    DTIC Science & Technology

    2013-08-01

    al., 2011). We then for the first time demonstrated that inhaled OT using a pediatric nebulizer effectively reaches the central nervous system of...rhesus macaques, providing a proof of concept for a clinical OT treatment method well-tolerated by children. Subsequently, we showed the inhaled OT...Objective 2: Determine how OFC neurons mediate ORPs. • Objective 3: Determine the effects of OFC perturbations on ORPs. • Objective 4

  2. Classification of odontogenic cysts and tumors – Antecedents

    PubMed Central

    Imran, Aesha; Jayanthi, P; Tanveer, Shahela; Gobu, Sreeja C

    2016-01-01

    Pierre Paul Broca produced a monograph on tumor classification which also included the classification of odontogenic tumors (OTs). The terminology used to describe malignant epithelial OTs has varied since the World Health Organization published the initial consensus on the taxonomy of OTs. Minor changes were introduced in the second edition. It is only in the very recent years that additional knowledge has accumulated and refined the classification. This review emphasizes on reasons for modification by each author and the recent acceptance. PMID:27601821

  3. Synthetic Aperture Radar Simulation Study

    DTIC Science & Technology

    1984-03-01

    multilook are discussed. A chapter is devoted to elevation and planimetric data bases. In addition, six- teen pictures of SAR images from Hughes Aircraft, as...scans. Figure 5.4-1 is a photograph ot two SAR displays. The tirst display is made up ot six subscans and has a multilook ot one. Note that tading is...dentfi by block number) * Synthetic Aperture Radar ( SAR ) Simulation Study Radar Simulation Data Bases 5/~t. 4th.- Computer Image Generation Display 20

  4. Effect of environmental pollutants on oxytocin synthesis and secretion from corpus luteum and on contractions of uterus from pregnant cows

    SciTech Connect

    Mlynarczuk, Jaroslaw; Wrobel, Michal H.; Kotwica, Jan

    2010-09-15

    Chloro-organic compounds are persistent environmental pollutants and affect many reproductive processes. Oxytocin (OT) synthesized in luteal cells is a local regulator of ovarian activity and uterine contractions. Therefore the effect of xenobiotics on the OT prohormone synthesis, secretion of OT and progesterone (P4) from luteal cells and on myometrial contractions during early pregnancy in cows was investigated. Luteal cells and myometrial strips from a cow at early pregnancy were treated with polychlorinated biphenyl 77 (PCB 77), dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE) and hexachlorocyclohexane (HCH) (1 or 10 ng/ml). The mRNA expression of neurophysin-I/oxytocin (NP-I/OT) and peptidyl-glycine-{alpha}-amidating mono-oxygenase (PGA) and concentration of OT and P4 were determined by RT-PCR and EIA, respectively. Moreover, the effect of xenobiotics given with P4 (12 ng/ml) on the basal and OT (10{sup -7} M) stimulated contractions of myometrial strips was studied. Xenobiotics increased (P < 0.05) OT secretion but DDE only stimulated P4 secretion. The ratio of P4 to OT in culture medium was decreased by all xenobiotics during 9-12 weeks of pregnancy. All xenobiotics, except HCH, increased (P < 0.05) mRNA expression of NP-I/OT during all stages of pregnancy and all treatments decreased (P < 0.05) expression of mRNA for PGA during 9-12 weeks of pregnancy. Myometrial strips were relaxed (P < 0.01) after pre-incubation with P4, while each of the xenobiotics jointly with P4 increased (P < 0.01) myometrial contractions. In conclusion, the xenobiotics used increased both expression of mRNA for genes involved in OT synthesis and secretion of OT from luteal cells. This decreases the ratio of P4 to OT and presumably, in this manner, the chloro-organic compounds can influence uterine contractions and enhance risk of abortions in pregnant females.

  5. The Home-Based Occupational Therapy Intervention in the Alzheimer's Disease Multiple Intervention Trial (ADMIT).

    PubMed

    Schmid, Arlene A; Spangler-Morris, Carrie; Beauchamp, Rachel C; Wellington, Miranda C; Hayden, Whitney M; Porterfield, Hannah S; Ferguson, Denisha; Callahan, Christopher M

    2015-01-01

    There is no way to prevent functional declines related to Alzheimer's Disease (AD). The use of occupational therapy (OT) has been shown to be successful in managing some aspects of AD. We added home-based OT to evidence-based best practice for AD with the aim of delaying functional decline in people with AD. OT was delivered in the home to a caregiver dyad including the person with AD and her/his caregiver. This paper describes the OT intervention for the AD Multiple Intervention Trial, a parallel randomized controlled trial. We include baseline data on the 180 caregiver dyads.

  6. Individual difference in serum oxytocin concentrations of calves and the correlation with those in dams.

    PubMed

    Chen, Siyu; Tanaka, Shigefumi; Oyakawa, Chisako; Roh, Sanggun; Sato, Shusuke

    2014-01-01

    We examined individual differences in serum oxytocin concentrations (OT) of calves, and assessed whether these differences were correlated with their dams' milk and serum OT. Eight Holstein (H), nine Japanese Shorthorn (JS), and six Japanese Black (JB) calves were examined. Blood was collected three times during the first month in H calves, while their dams' blood was collected three times prior to parturition. Milk was collected twice after parturition from H cows. Blood from JS and JB calves were collected at 1 and 4 months old, while of their dams only once before parturition. Serum OT in H calves at 7 days old was significantly correlated with that at 30 days. Serum OT of JS calves at 1 month old was significantly correlated to that at 4 months, while of JB calves was also positively correlated (r = 0.70). Serum OT of calves showed significant individual differences in each breed. Serum OT of calves was not correlated with the milk OT of dams, except for 3-day-old calves that fed on their dams' milk. We concluded that although serum OT differed among individuals, this difference was stable within each individual and not affected by the serum OT of the dams.

  7. A common oxytocin receptor gene (OXTR) polymorphism modulates intranasal oxytocin effects on the neural response to social cooperation in humans

    PubMed Central

    Feng, Chunliang; Lori, Adriana; Waldman, Irwin D.; Binder, Elisabeth B.; Haroon, Ebrahim; Rilling, James K.

    2015-01-01

    Intranasal oxytocin (OT) can modulate social-emotional functioning and related brain activity in humans. Consequently, OT has been discussed as a potential treatment for psychiatric disorders involving social behavioral deficits. However, OT effects are often heterogeneous across individuals. Here we explore individual differences in OT effects on the neural response to social cooperation as a function of the rs53576 polymorphism of the oxytocin receptor gene (OXTR). Previously, we conducted a double-blind, placebo-controlled study in which healthy men and women were randomized to treatment with intranasal OT or placebo. Afterwards, they were imaged with fMRI while playing an iterated Prisoner’s Dilemma Game with same-sex partners. Within the left ventral caudate nucleus, intranasal OT treatment increased activation to reciprocated cooperation in men, but tended to decrease activation in women. Here, we show that these sex differences in OT effects are specific to individuals with the rs53576 GG genotype, and are not found for other genotypes (rs53576 AA/AG). Thus, OT may increase the reward or salience of positive social interactions for male GG homozygotes, while decreasing those processes for female GG homozygotes. These results suggest that rs53576 genotype is an important variable to consider in future investigations of the clinical efficacy of intranasal OT treatment. PMID:26178189

  8. Influence of oxytocin on emotion recognition from body language: A randomized placebo-controlled trial.

    PubMed

    Bernaerts, Sylvie; Berra, Emmely; Wenderoth, Nicole; Alaerts, Kaat

    2016-10-01

    The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. One mechanism by which OT is hypothesized to promote prosocial behavior is by enhancing the processing of socially relevant information from the environment. With the present study, we explored to what extent OT can alter the 'reading' of emotional body language as presented by impoverished biological motion point light displays (PLDs). To do so, a double-blind between-subjects randomized placebo-controlled trial was conducted, assessing performance on a bodily emotion recognition task in healthy adult males before and after a single-dose of intranasal OT (24 IU). Overall, a single-dose of OT administration had a significant effect of medium size on emotion recognition from body language. OT-induced improvements in emotion recognition were not differentially modulated by the emotional valence of the presented stimuli (positive versus negative) and also, the overall tendency to label an observed emotional state as 'happy' (positive) or 'angry' (negative) was not modified by the administration of OT. Albeit moderate, the present findings of OT-induced improvements in bodily emotion recognition from whole-body PLD provide further support for a link between OT and the processing of socio-communicative cues originating from the body of others.

  9. A common oxytocin receptor gene (OXTR) polymorphism modulates intranasal oxytocin effects on the neural response to social cooperation in humans.

    PubMed

    Feng, C; Lori, A; Waldman, I D; Binder, E B; Haroon, E; Rilling, J K

    2015-09-01

    Intranasal oxytocin (OT) can modulate social-emotional functioning and related brain activity in humans. Consequently, OT has been discussed as a potential treatment for psychiatric disorders involving social behavioral deficits. However, OT effects are often heterogeneous across individuals. Here we explore individual differences in OT effects on the neural response to social cooperation as a function of the rs53576 polymorphism of the oxytocin receptor gene (OXTR). Previously, we conducted a double-blind, placebo-controlled study in which healthy men and women were randomized to treatment with intranasal OT or placebo. Afterwards, they were imaged with functional magnetic resonance imaging while playing an iterated Prisoner's Dilemma Game with same-sex partners. Within the left ventral caudate nucleus, intranasal OT treatment increased activation to reciprocated cooperation in men, but tended to decrease activation in women. Here, we show that these sex differences in OT effects are specific to individuals with the rs53576 GG genotype, and are not found for other genotypes (rs53576 AA/AG). Thus, OT may increase the reward or salience of positive social interactions for male GG homozygotes, while decreasing those processes for female GG homozygotes. These results suggest that rs53576 genotype is an important variable to consider in future investigations of the clinical efficacy of intranasal OT treatment.

  10. Oxytocin knockout mice demonstrate enhanced intake of sweet and nonsweet carbohydrate solutions.

    PubMed

    Sclafani, Anthony; Rinaman, Linda; Vollmer, Regis R; Amico, Janet A

    2007-05-01

    Oxytocin knockout (OT KO) mice display enhanced intake of nutritive and nonnutritive sweet solutions (i.e., sucrose and saccharin) compared with wild-type (WT) mice of the same C57BL/6 background strain. The present study further investigated the differential behavioral response of OT KO and WT mice to sucrose solutions and also examined intake preferences of OT KO and WT mice for palatable but nonsweet isocaloric solutions of carbohydrate and fat. A progressive ratio operant licking procedure demonstrated that OT KO and WT mice display a similar motivational drive to consume 10% sucrose. A series of two-bottle intake tests revealed that OT KO mice consume significantly larger amounts of both sweet and nonsweet carbohydrate solutions (i.e., sucrose, Polycose, and cornstarch) compared with WT cohorts. Intake pattern analyses revealed that OT KO mice overconsume carbohydrate solutions by initiating more drinking bouts compared with WT mice; bout sizes did not differ between the genotypes. In contrast, OT KO and WT mice did not differ in their intake of Intralipid, a palatable soybean oil emulsion. These findings indicate that the absence of OT in mice does not affect their appetitive drive to consume palatable sucrose solutions. Instead, the absence of OT may increase daily intake of palatable sweet and nonsweet solutions of carbohydrate (but not fat) by selectively blunting or masking processes that contribute to postingestive satiety.

  11. Involvement of the oxytocin system in the bed nucleus of the stria terminalis in the sex-specific regulation of social recognition

    PubMed Central

    Dumais, Kelly M.; Alonso, Andrea G.; Immormino, Marisa A.; Bredewold, Remco; Veenema, Alexa H.

    2015-01-01

    Sex differences in the oxytocin (OT) system in the brain may explain why OT often regulates social behaviors in sex-specific ways. However, a link between sex differences in the OT system and sex-specific regulation of social behavior has not been tested. Here, we determined whether sex differences in the OT receptor (OTR) or in OT release in the posterior bed nucleus of the stria terminalis (pBNST) mediates sex-specific regulation of social recognition in rats. We recently showed that, compared to female rats, male rats have a three-fold higher OTR binding density in the pBNST, a sexually dimorphic area implicated in the regulation of social behaviors. We now demonstrate that OTR antagonist (5 ng/0.5 μl/side) administration into the pBNST impairs social recognition in both sexes, while OT (100 pg/0.5 μl/side) administration into the pBNST prolongs the duration of social recognition in males only. These effects seem specific to social recognition, as neither treatment altered total social investigation time in either sex. Moreover, baseline OT release in the pBNST, as measured with in vivo microdialysis, did not differ between the sexes. However, males showed higher OT release in the pBNST during social recognition compared to females. These findings suggest a sex-specific role of the OT system in the pBNST in the regulation of social recognition. PMID:26630388

  12. Sniff and mimic - Intranasal oxytocin increases facial mimicry in a sample of men.

    PubMed

    Korb, Sebastian; Malsert, Jennifer; Strathearn, Lane; Vuilleumier, Patrik; Niedenthal, Paula

    2016-08-01

    The neuropeptide oxytocin (OT) has many potential social benefits. For example, intranasal administration of OT appears to trigger caregiving behavior and to improve the recognition of emotional facial expressions. But the mechanism for these effects is not yet clear. Recent findings relating OT to action imitation and to the visual processing of the eye region of faces point to mimicry as a mechanism through which OT improves processing of emotional expression. To test the hypothesis that increased levels of OT in the brain enhance facial mimicry, 60 healthy male participants were administered, in a double-blind between-subjects design, 24 international units (IUs) of OT or placebo (PLA) through nasal spray. Facial mimicry and emotion judgments were recorded in response to movie clips depicting changing facial expressions. As expected, facial mimicry was increased in the OT group, but effects were strongest for angry infant faces. These findings provide further evidence for the importance of OT in social cognitive skills, and suggest that facial mimicry mediates the effects of OT on improved emotion recognition.

  13. Oxytocin reverses amphetamine-induced deficits in social bonding: evidence for an interaction with nucleus accumbens dopamine.

    PubMed

    Young, Kimberly A; Liu, Yan; Gobrogge, Kyle L; Wang, Hui; Wang, Zuoxin

    2014-06-18

    Drug addiction has devastating consequences on social behaviors and can lead to the impairment of social bonding. Accumulating evidence indicates that alterations in oxytocin (OT) and dopamine (DA) neurotransmission within brain reward circuitry may be involved. We investigated this possibility, as well as the therapeutic potential of OT for drug-induced social deficits, using the prairie vole (Microtus ochrogaster)-a socially monogamous rodent that forms enduring pair bonds between adult mates. We demonstrate that repeated exposure to the commonly abused psychostimulant amphetamine (AMPH) inhibits the formation of partner preferences (an index of pair bonding) in female prairie voles. AMPH exposure also altered OT and DA neurotransmission in regions that mediate partner preference formation: it decreased OT and DA D2 receptor immunoreactivity in the medial prefrontal cortex (mPFC) and nucleus accumbens (NAcc), respectively, and increased NAcc DA levels. Administration of OT directly into the mPFC of AMPH-exposed voles restored partner preferences, and altered NAcc DA levels, and this effect was dependent on OT receptor activation. Together, these data suggest that repeated AMPH exposure impairs pair bonding through an OT-mediated mechanism, and that OT and DA systems within brain reward circuitry may interact to mediate the complex relationship between drug abuse and social bonding. Further, these results provide empirical support for the idea that the central OT system may represent an important target for the treatment of social deficits in addiction.

  14. Melanocortin Receptor Agonists Facilitate Oxytocin-Dependent Partner Preference Formation in the Prairie Vole.

    PubMed

    Modi, Meera E; Inoue, Kiyoshi; Barrett, Catherine E; Kittelberger, Kara A; Smith, Daniel G; Landgraf, Rainer; Young, Larry J

    2015-07-01

    The central melanocortin (MC) system has been widely studied for its effects on food intake and sexual behavior. However, the MC system, and more specifically the MC4 receptor (MC4R), also interacts with neurochemical systems that regulate socioemotional behaviors, including oxytocin (OT) and dopamine. In monogamous prairie voles, OT and dopamine interact to promote partner preference formation, a laboratory measure of an enduring social bond between mates. Here we investigated the effects of MC receptor activation on partner preference formation in prairie voles, as well as the interaction between the MC and OT systems during this process. Peripheral administration of the brain penetrant MC3/4R receptor peptide agonist, Melanotan II (MTII), and the highly selective, small-molecule MC4R agonist, Pf-446687, enhanced partner preference formation in the prairie vole, but not in the non-monogamous meadow vole. MTII-induced partner preferences were enduring, as they were present 1 week after drug manipulation. The prosocial effects of MCR agonists may be mediated, in part, through modulation of OT, as coadministration of an OT receptor antagonist prevented MTII-induced partner preferences. MTII also selectively activated hypothalamic OT neurons and potentiated central OT release. As OT has been shown to enhance some aspects of social cognition in humans, our data suggest that the MC4R may be a viable therapeutic target for enhancing social function in psychiatric disorders, including autism spectrum disorders and schizophrenia, potentially through activation of the OT system.

  15. Identification of the neuropeptide content of individual rat neurohypophysial terminals.

    PubMed

    Custer, Edward E; Ortiz-Miranda, Sonia; Knott, Thomas K; Rawson, Randi; Elvey, Christian; Lee, Ryan H; Lemos, José R

    2007-07-30

    The objective of this study was to develop a method that could reliably determine the arginine vasopressin (AVP) and/or oxytocin (OT) content of individual rat neurohypophysial terminals (NHT) >or=5 microm in diameter, the size used for electrophysiological recordings. We used a commercially available, highly sensitive enzyme-linked immunoassay (ELISA) kit with a sensitivity of 0.25 pg to AVP and of 1.0pg to OT. The NHT content of AVP (2.21+/-0.10 pg) was greater than OT (1.77+/-0.08 pg) and increased with terminal size. AVP-positive terminals (10.2+/-0.21 microm) were larger in diameter than OT-positive terminals (9.1+/-0.24 microm). Immunocytochemical techniques indicated that a higher percentage (58%) of smaller terminals contained OT, and that a higher percentage (42%) of larger NHTs were colabeled. Similar percentages of AVP-positive terminals were obtained between immunocytochemical (73%) and ELISA (72%) methods when NHTs were assayed for AVP alone, but there was a higher percentage of OT terminals when using immunocytochemistry (43%) compared to ELISA (26%). The percent of AVP-positive (60%) and OT-positive (18%) terminals decreased when NHT were assayed for both AVP and OT. Therefore, the best method to reliably identify AVP-positive NHTs is to assay only for AVP, since this allows the conclusion that AVP-negative terminals contain only OT.

  16. Tendon Injuries

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  17. Preventing Tech Aches: Using Smart Phones Wisely

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  18. Returning to Work

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  19. Learning Through Play

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  20. About Occupational Therapy

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  1. American Occupational Therapy Association

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  2. Chronic Pain

    MedlinePlus

    ... Ethics Occupational Therapy Assistants Advocacy & Policy New Evaluation Codes Learn how to use the new OT CPT® Evaluation codes correctly . Congressional Affairs AOTPAC Federal Regulatory Affairs Health ...

  3. Oxytocin Reverses Amphetamine-Induced Deficits in Social Bonding: Evidence for an Interaction with Nucleus Accumbens Dopamine

    PubMed Central

    Liu, Yan; Gobrogge, Kyle L.; Wang, Hui

    2014-01-01

    Drug addiction has devastating consequences on social behaviors and can lead to the impairment of social bonding. Accumulating evidence indicates that alterations in oxytocin (OT) and dopamine (DA) neurotransmission within brain reward circuitry may be involved. We investigated this possibility, as well as the therapeutic potential of OT for drug-induced social deficits, using the prairie vole (Microtus ochrogaster)—a socially monogamous rodent that forms enduring pair bonds between adult mates. We demonstrate that repeated exposure to the commonly abused psychostimulant amphetamine (AMPH) inhibits the formation of partner preferences (an index of pair bonding) in female prairie voles. AMPH exposure also altered OT and DA neurotransmission in regions that mediate partner preference formation: it decreased OT and DA D2 receptor immunoreactivity in the medial prefrontal cortex (mPFC) and nucleus accumbens (NAcc), respectively, and increased NAcc DA levels. Administration of OT directly into the mPFC of AMPH-exposed voles restored partner preferences, and altered NAcc DA levels, and this effect was dependent on OT receptor activation. Together, these data suggest that repeated AMPH exposure impairs pair bonding through an OT-mediated mechanism, and that OT and DA systems within brain reward circuitry may interact to mediate the complex relationship between drug abuse and social bonding. Further, these results provide empirical support for the idea that the central OT system may represent an important target for the treatment of social deficits in addiction. PMID:24948805

  4. 77 FR 8066 - Semiannual Agenda of Regulations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-13

    ... nomenclature or address changes), and eliminating those OTS regulations for which other appropriate Federal... or securitization satisfies all of the conditions for sale accounting treatment under...

  5. Adaptive coding of the value of social cues with oxytocin, an fMRI study in autism spectrum disorder.

    PubMed

    Andari, Elissar; Richard, Nathalie; Leboyer, Marion; Sirigu, Angela

    2016-03-01

    The neuropeptide oxytocin (OT) is one of the major targets of research in neuroscience, with respect to social functioning. Oxytocin promotes social skills and improves the quality of face processing in individuals with social dysfunctions such as autism spectrum disorder (ASD). Although one of OT's key functions is to promote social behavior during dynamic social interactions, the neural correlates of this function remain unknown. Here, we combined acute intranasal OT (IN-OT) administration (24 IU) and fMRI with an interactive ball game and a face-matching task in individuals with ASD (N = 20). We found that IN-OT selectively enhanced the brain activity of early visual areas in response to faces as compared to non-social stimuli. OT inhalation modulated the BOLD activity of amygdala and hippocampus in a context-dependent manner. Interestingly, IN-OT intake enhanced the activity of mid-orbitofrontal cortex in response to a fair partner, and insula region in response to an unfair partner. These OT-induced neural responses were accompanied by behavioral improvements in terms of allocating appropriate feelings of trust toward different partners' profiles. Our findings suggest that OT impacts the brain activity of key areas implicated in attention and emotion regulation in an adaptive manner, based on the value of social cues.

  6. Otophylloside B Protects Against Aβ Toxicity in Caenorhabditis elegans Models of Alzheimer's Disease.

    PubMed

    Yang, Jie; Huang, Xiao-Bing; Wan, Qin-Li; Ding, Ai-Jun; Yang, Zhong-Lin; Qiu, Ming-Hua; Sun, Hua-Ying; Qi, Shu-Hua; Luo, Huai-Rong

    2017-02-13

    Alzheimer's disease (AD) is a major public health concern worldwide and the few drugs currently available only treat the symptoms. Hence, there is a strong need to find more effective anti-AD agents. Cynanchum otophyllum is a traditional Chinese medicine for treating epilepsy, and otophylloside B (Ot B), isolated from C. otophyllum, is the essential active component. Having previously identified anti-aging effects of Ot B, we evaluated Ot B for AD prevention in C. elegans models of AD and found that Ot B extended lifespan, increased heat stress-resistance, delayed body paralysis, and increased the chemotaxis response. Collectively, these results indicated that Ot B protects against Aβ toxicity. Further mechanistic studies revealed that Ot B decreased Aβ deposition by decreasing the expression of Aβ at the mRNA level. Genetic analyses showed that Ot B mediated its effects by increasing the activity of heat shock transcription factor (HSF) by upregulating the expression of hsf-1 and its target genes, hsp-12.6, hsp-16.2 and hsp-70. Ot B also increased the expression of sod-3 by partially activating DAF-16, while SKN-1 was not essential in Ot B-mediated protection against Aβ toxicity.

  7. Twenty years of oxygenated treatment on Russian supercritical plants

    SciTech Connect

    Shitsman, M.E.

    1995-01-01

    The oxygenated treatment (OT) in Russia was first (1973-74) introduced at the 300 MWe Konakovo Supercritical units (SCU). The advantage of OT over AVT especially in rising the reliability of low-radiation boiler section (LRS) promoted to conversion the working units on OT and putting into operation the new SCUs directly with OT. All Russian supercritical plants, besides two of them, operate currently with OT. The conversion of units on OT doesn`t demand much expenditures. OT is simple and reliable. The OT introduction makes the main difficulty connected with the existance of stable stereotype for ammonia feed and supporting the oxygen concentration in feedwater less than 5 ppb. The staff of two mentioned plants gave proof of devotion to AVT by its wide application in the USA and Japan. Now the units with once through boilers at these countries are intensively conversed on OT. It is possible to hope that this circumstance will dismiss at last the stereotype of oxygen fear.

  8. Combining oxytocin administration and positive emotion inductions: Examining social perception and analytical performance.

    PubMed

    Human, Lauren J; Thorson, Katherine R; Woolley, Joshua D; Mendes, Wendy Berry

    2017-03-17

    Intranasal administration of the hypothalamic neuropeptide oxytocin (OT) has, in some studies, been associated with positive effects on social perception and cognition. Similarly, positive emotion inductions can improve a range of perceptual and performance-based behaviors. In this exploratory study, we examined how OT administration and positive emotion inductions interact in their associations with social and analytical performance. Participants (N=124) were randomly assigned to receive an intranasal spray of OT (40IU) or placebo and then viewed one of three videos designed to engender one of the following emotion states: social warmth, pride, or an affectively neutral state. Following the emotion induction, participants completed social perception and analytical tasks. There were no significant main effects of OT condition on social perception tasks, failing to replicate prior research, or on analytical performance. Further, OT condition and positive emotion inductions did not interact with each other in their associations with social perception performance. However, OT condition and positive emotion manipulations did significantly interact in their associations with analytical performance. Specifically, combining positive emotion inductions with OT administration was associated with worse analytical performance, with the pride induction no longer benefiting performance and the warmth induction resulting in worse performance. In sum, we found little evidence for main or interactive effects of OT on social perception but preliminary evidence that OT administration may impair analytical performance when paired with positive emotion inductions.

  9. Oxytocin facilitates accurate perception of competition in men and kinship in women.

    PubMed

    Fischer-Shofty, Meytal; Levkovitz, Yechiel; Shamay-Tsoory, Simone G

    2013-03-01

    Despite the dominant role of the hormone oxytocin (OT) in social behavior, little is known about the role of OT in the perception of social relationships. Furthermore, it is unclear whether there are sex differences in the way that OT affects social perception. Here, we employed a double-blind, placebo-controlled crossover design to investigate the effect of OT on accurate social perception. Following treatment, 62 participants completed the Interpersonal Perception Task, a method of assessing the accuracy of social judgments that requires identification of the relationship between people interacting in real life video clips divided into three categories: kinship, intimacy and competition. The findings suggest that OT had a general effect on improving accurate perception of social interactions. Furthermore, we show that OT also involves sex-specific characteristics. An interaction between treatment, task category and sex indicated that OT had a selective effect on improving kinship recognition in women, but not in men, whereas men's performance was improved following OT only for competition recognition. It is concluded that the gender-specific findings reported here may point to some biosocial differences in the effect of OT which may be expressed in women's tendency for communal and familial social behavior as opposed to men's tendency for competitive social behavior.

  10. Oxytocin facilitates accurate perception of competition in men and kinship in women

    PubMed Central

    Fischer-Shofty, Meytal; Levkovitz, Yechiel

    2013-01-01

    Despite the dominant role of the hormone oxytocin (OT) in social behavior, little is known about the role of OT in the perception of social relationships. Furthermore, it is unclear whether there are sex differences in the way that OT affects social perception. Here, we employed a double-blind, placebo-controlled crossover design to investigate the effect of OT on accurate social perception. Following treatment, 62 participants completed the Interpersonal Perception Task, a method of assessing the accuracy of social judgments that requires identification of the relationship between people interacting in real life video clips divided into three categories: kinship, intimacy and competition. The findings suggest that OT had a general effect on improving accurate perception of social interactions. Furthermore, we show that OT also involves sex-specific characteristics. An interaction between treatment, task category and sex indicated that OT had a selective effect on improving kinship recognition in women, but not in men, whereas men's performance was improved following OT only for competition recognition. It is concluded that the gender-specific findings reported here may point to some biosocial differences in the effect of OT which may be expressed in women's tendency for communal and familial social behavior as opposed to men's tendency for competitive social behavior. PMID:22446301

  11. Time course of the estradiol-dependent induction of oxytocin receptor binding in the ventromedial hypothalamic nucleus of the rat

    SciTech Connect

    Johnson, A.E.; Ball, G.F.; Coirini, H.; Harbaugh, C.R.; McEwen, B.S.; Insel, T.R. )

    1989-09-01

    Oxytocin (OT) transmission is involved in the steroid-dependent display of sexual receptivity in rats. One of the biochemical processes stimulated by the ovarian steroid 17 beta-estradiol (E2) that is relevant to reproduction is the induction of OT receptor binding in the ventromedial hypothalamic nucleus (VMN). The purpose of these experiments was to determine if E2-induced changes in OT receptor binding in the VMN occur within a time frame relevant to cyclic changes in ovarian steroid secretion. OT receptor binding was measured in the VMN of ovariectomized rats implanted for 0-96 h with E2-containing Silastic capsules. The rate of decay of OT receptor binding was measured in another group of animals 6-48 h after capsule removal. Receptors were labeled with the specific OT receptor antagonist ({sup 125}I)d(CH2)5(Tyr(Me)2,Thr4,Tyr-NH2(9))OVT, and binding was measured with quantitative autoradiographic methods. In addition, plasma E2 levels and uterine weights were assessed in animals from each treatment condition. Significant increases in E2-dependent OT receptor binding and uterine weight occurred within 24 h of steroid treatment. After E2 withdrawal, OT receptor binding and uterine weight decreased significantly within 24 h. These results are consistent with the hypothesis that steroid modulation of OT receptor binding is necessary for the induction of sexual receptivity.

  12. Association of oxytocin level and less severe forms of childhood maltreatment history among healthy Japanese adults involved with child care

    PubMed Central

    Mizuki, Rie; Fujiwara, Takeo

    2015-01-01

    Background: Oxytocin (OT) is known to play a role in stress regulation. The association between childhood maltreatment history and neuropeptide OT concentration is inconsistent due to the varying degrees of severity of childhood maltreatment, among other contributing factors. Less severe forms of childhood maltreatment history might enhance OT concentrations as a response to coping with social stress within the family. The purpose of this study is to investigate the association between less severe forms of childhood maltreatment history and OT concentrations among healthy adults. Method: Eighty adults (49 women and 31 men) with 18- to 48-month-old children were recruited using a snowball sample in Tokyo, Japan. Urine samples were collected for OT measurement. Less severe (low and moderate) childhood maltreatment history, including physical abuse, physical neglect, emotional abuse, emotional neglect, and sexual abuse, was assessed using the self-report questionnaire, the Childhood Trauma Questionnaire. Results: Less severe physical abuse was significantly associated with higher OT concentration after adjusting for age (p = 0.014). Also, less severe forms of physical abuse were independently significantly associated with higher OT concentration after controlling for other types of childhood maltreatment (p = 0.027). A positive dose-response association between the number of less severe childhood maltreatment types and OT concentration was observed (p = 0.031). Conclusion: A history of less severe forms of childhood physical abuse was associated with higher OT concentration in healthy adults. Poly-victimization of several types of less severe childhood maltreatment was also associated with higher OT concentrations. Less severe forms of childhood maltreatment might enhance OT concentrations in order to cope with social stress. PMID:26157369

  13. Orthostatic Tremor and Orthostatic Myoclonus: Weight-bearing Hyperkinetic Disorders: A Systematic Review, New Insights, and Unresolved Questions

    PubMed Central

    Hassan, Anhar; van Gerpen, Jay A.

    2016-01-01

    Background Orthostatic tremor (OT) and orthostatic myoclonus (OM) are weight-bearing hyperkinetic movement disorders most commonly affecting older people that induce “shaky legs” upon standing. OT is divided into “classical” and “slow” forms based on tremor frequency. In this paper, the first joint review of OT and OM, we review the literature and compare and contrast their demographic, clinical, electrophysiological, neuroimaging, pathophysiological, and treatment characteristics. Methods A PubMed search up to July 2016 using the phrases “orthostatic tremor,” “orthostatic myoclonus,” “shaky legs,” and “shaky legs syndrome” was performed. Results OT and OM should be suspected in older patients reporting unsteadiness with prolonged standing and/or who exhibit cautious, wide-based gaits. Surface electromyography (SEMG) is necessary to verify the diagnoses. Functional neuroimaging and electrophysiology suggest the generator of classical OT lies within the cerebellothalamocortical network. For OM, and possibly slow OT, the frontal, subcortical cerebrum is the most likely origin. Clonazepam is the most useful medication for classical OT, and levetiracetam for OM, although results are often disappointing. Deep brain stimulation appears promising for classical OT. Rolling walkers reliably improve gait affected by these disorders, as both OT and OM attenuate when weight is transferred from the legs to the arms. Discussion Orthostatic hyperkinesias are likely underdiagnosed, as SEMG is often unavailable in clinical practice, and thus may be more frequent than currently recognized. The shared weight-bearing induction of OT and OM may indicate a common pathophysiology. Further research, including use of animal models, is necessary to better define the prevalence and pathophysiology of OT and OM, in order to improve their treatment, and provide additional insights into basic balance and gait mechanisms. PMID:28105385

  14. Long-term oxytocin administration enhances the experience of attachment.

    PubMed

    Bernaerts, Sylvie; Prinsen, Jellina; Berra, Emmely; Bosmans, Guy; Steyaert, Jean; Alaerts, Kaat

    2017-04-01

    The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. Previous studies showed that a single-dose of exogenously administered OT can affect trust and feelings of attachment insecurity. With the present study, we explored the effects of two weeks of daily OT administration on measures of state and trait attachment using a double-blind between-subjects randomized placebo-controlled design. In 40 healthy young adult men state and trait attachment were assessed before and after two weeks of daily intranasal OT (24 IU) or placebo using the State Adult Attachment Scale and the Inventory of Parent and Peer Attachment. Mood, social responsiveness and quality of life were additionally assessed as secondary outcome measures. Reductions in attachment avoidance and increases in reports of attachment toward peers were reported after two weeks of OT treatment. Further, treatment-induced changes were most pronounced for participants with less secure attachment towards their peers. indicating that normal variance at baseline modulated treatment response. OT treatment was additionally associated with changes in mood, indicating decreases in feelings of tension and (tentatively) anger in the OT group, not in the placebo group. Further, at the end of the two-week trial, both treatment groups (OT, placebo) reported to experience an increase in social responsiveness and quality of life, but the effects were only specific to the OT-treatment in terms of reports on 'social motivation'. In summary, the observed improvements on state and trait dimensions of attachment after a multiple-dose treatment with OT provide further evidence in support of a pivotal role of OT in promoting the experience of attachment.

  15. Oxytocin reduces anxiety via ERK1/2 activation: local effect within the rat hypothalamic paraventricular nucleus.

    PubMed

    Blume, Annegret; Bosch, Oliver J; Miklos, Sandra; Torner, Luz; Wales, Lynn; Waldherr, Martin; Neumann, Inga D

    2008-04-01

    The neuropeptide oxytocin (OT) modulates social behaviours and is an important anxiolytic substance of the brain. However, sites of action and the intracellular signalling pathways downstream of OT receptors (OTR) within the brain remain largely unknown. In the present studies, we localized the anxiolytic effect of OT by bilateral microinfusion of OT (0.01 nmol/0.5 microL) into the hypothalamic paraventricular nucleus (PVN) in male rats using both the elevated plus-maze and the light-dark box. Moreover, intracerebroventricular administration of OT, but not of the related neuropeptide vasopressin (VP), dose-dependently activated the extracellular signal-regulated kinase 1/2 (ERK1/2) cascade. Specifically, OT induced the phosphorylation of Raf-1, MEK1/2 and ERK1/2 in the hypothalamus in vivo and in hypothalamic H32 neurons via EGF receptors. OT-induced ERK1/2 phosphorylation was immunohistochemically localized within VP neurons of the PVN and the supraoptic nucleus. Importantly, the anxiolytic effect of OT within the PVN was prevented by local inhibition of the MAP kinase cascade with a MEK1/2 inhibitor (U0126, 0.5 nmol/0.5 microL) locally infused prior to OT, indicating the causal involvement of this intracellular signalling cascade in the behavioural effect of OT. OT effects within the hypothalamus may have far-reaching implications for the regulation of emotionality and social behaviours and, consequently, for the development of possible therapeutic strategies to treat affective disorders. Thus, OTR agonism or activation of the ERK1/2 cascade, specifically within the hypothalamus, may provide therapeutically relevant mechanisms.

  16. Intranasal oxytocin effects on social cognition: a critique.

    PubMed

    Evans, Simon L; Dal Monte, Olga; Noble, Pamela; Averbeck, Bruno B

    2014-09-11

    The last decade has seen a large number of published findings supporting the hypothesis that intranasally delivered oxytocin (OT) can enhance the processing of social stimuli and regulate social emotion-related behaviors such as trust, memory, fidelity, and anxiety. The use of nasal spray for administering OT in behavioral research has become a standard method, but many questions still exist regarding its action. OT is a peptide that cannot cross the blood-brain barrier, and it has yet to be shown that it does indeed reach the brain when delivered intranasally. Given the evidence, it seems highly likely that OT does affect behavior when delivered as a nasal spray. These effects may be driven by at least three possible mechanisms. First, the intranasally delivered OT may diffuse directly into the CNS where it directly engages OT receptors. Second, the intranasally delivered OT may trigger increased central release via an indirect peripheral mechanism. And third, the indirect peripheral effects may directly lead to behavioral effects via some mechanism other than increased central release. Although intranasally delivered OT likely affects behavior, there are conflicting reports as to the exact nature of those behavioral changes: some studies suggest that OT effects are not always "pro-social" and others suggest effects on social behaviors are due to a more general anxiolytic effect. In this critique, we draw from work in healthy human populations and the animal literature to review the mechanistic aspects of intranasal OT delivery, and to discuss intranasal OT effects on social cognition and behavior. We conclude that future work should control carefully for anxiolytic and gender effects, which could underlie inconsistencies in the existing literature. This article is part of a Special Issue entitled Oxytocin and Social Behav.

  17. Plasma and CSF oxytocin levels after intranasal and intravenous oxytocin in awake macaques.

    PubMed

    Freeman, Sara M; Samineni, Sridhar; Allen, Philip C; Stockinger, Diane; Bales, Karen L; Hwa, Granger G C; Roberts, Jeffrey A

    2016-04-01

    Oxytocin (OT) is a neuropeptide that mediates a variety of complex social behaviors in animals and humans. Intranasal OT has been used as an experimental therapeutic for human conditions characterized by deficits in social functioning, especially autism spectrum disorder and schizophrenia. However, it is currently under intense debate whether intranasal delivery of OT reaches the central nervous system. In this study, four female rhesus macaques were implanted with chronic intrathecal catheters and used to investigate the pharmacokinetic profile of OT in the central nervous system and the peripheral vasculature following intravenous (IV) and intranasal (IN) administration of OT. In a randomized, crossover design, OT was given to four awake monkeys at three different doses based on body weight (0.1 IU/kg; 1 IU/kg; 5 IU/kg). A time course of concurrent cerebrospinal fluid (CSF) and plasma samples were taken following administration. We found a dose-dependent effect of IV OT treatment on plasma OT levels, which peaked at 5 min post-dose and gradually returned to baseline by 120 min. In contrast, a change in CSF OT was only observed at the highest IV dose (5 IU/kg) at 15 min post-dose and gradually returned to baseline by 120 min. After IN administration, there was no significant change in plasma OT at any of the three doses. However, at the highest dose level, we found a significant increase in CSF OT at 15-30 min post- dose. The results of this study in light of recent, similar publications highlight the importance of methodological consistency across studies. This study also establishes a non-human primate model that can provide a stable platform for carrying out serial sampling from the central nervous system and peripheral vasculature concurrently.

  18. Positive reinforcing effect of oxytocin microinjection in the rat central nucleus of amygdala.

    PubMed

    László, K; Kovács, A; Zagoracz, O; Ollmann, T; Péczely, L; Kertes, E; Lacy, D G; Lénárd, L

    2016-01-01

    Neuropeptide oxytocin (OT) receives increasing attention since, it plays a role in various behaviors including anxiety, drug addiction, learning, social recognition, empathy, pair bonding and decreased aggression. The central nucleus of the amygdala (CeA), part of the limbic system, plays an important role in learning, memory, anxiety and reinforcing mechanisms. CeA was shown to be rich in OT-receptors (OTR). The aim of our study was to examine the possible effects of OT and OTR antagonist in the CeA on reinforcement using the conditioned place preference test and on anxiety using the elevated plus maze test. Male Wistar rats were microinjected bilaterally with 10 ng OT or 100 ng OT (Sigma: O6379, injected in volume of 0.4μl) or 10ng OTR antagonist (Sigma: L-2540) alone, or OTR antagonist 15 min prior 10 ng OT treatment or vehicle solution into the CeA. Rats receiving 10 ng OT spent significantly more time in the treatment quadrant during the test session, while 100 ng OT treatment produced no effect. Prior treatment with the non-peptide OTR antagonist blocked the effects of OT. The antagonist in itself did not influence the place preference. The elevated plus maze test revealed that 10 ng OT significantly increased the time spent in the open arms. OTR antagonist pre-treatment could inhibit this effect and the antagonist in itself did not affect the time spent in the open arms. Our results show that in the rat CeA OT has dose-dependent, positive reinforcing and anxiolytic effects, via OTR demonstrated by the blocking effects of selective OTR antagonist.

  19. Oxytocin regulates gastrointestinal motility, inflammation, macromolecular permeability, and mucosal maintenance in mice

    PubMed Central

    Margolis, Kara G.; Li, Zhishan; Gershon, Michael D.

    2014-01-01

    Enteric neurons express oxytocin (OT); moreover, enteric neurons and enterocytes express developmentally regulated OT receptors (OTRs). Although OT (with secretin) opposes intestinal inflammation, physiological roles played by enteric OT/OTR signaling have not previously been determined. We tested hypotheses that OT/OTR signaling contributes to enteric nervous system (ENS)-related gastrointestinal (GI) physiology. GI functions and OT effects were compared in OTR-knockout (OTRKO) and wild-type (WT) mice. Stool mass and water content were greater in OTRKO mice than in WT. GI transit time in OTRKO animals was faster than in WT; OT inhibited in vitro generation of ENS-dependent colonic migrating motor complexes in WT but not in OTRKO mice. Myenteric neurons were hyperplastic in OTRKO animals, and mucosal exposure to cholera toxin (CTX) in vitro activated Fos in more myenteric neurons in OTRKO than WT than in WT mice; OT inhibited the CTX response in WT but not in OTRKO mice. Villi and crypts were shorter in OTRKO than in WT mice, and transit-amplifying cell proliferation in OTRKO crypts was deficient. Macromolecular intestinal permeability in OTRKO was greater than WT mice, and experimental colitis was more severe in OTRKO mice; moreover, OT protected WT animals from colitis. Observations suggest that OT/OTR signaling acts as a brake on intestinal motility, decreases mucosal activation of enteric neurons, and promotes enteric neuronal development and/or survival. It also regulates proliferation of crypt cells and mucosal permeability; moreover OT/OTR signaling is protective against inflammation. Oxytocinergic signaling thus appears to play an important role in multiple GI functions that are subject to neuronal regulation. PMID:25147234

  20. Oxytocin affects spontaneous neural oscillations in trauma-exposed war veterans

    PubMed Central

    Eidelman-Rothman, Moranne; Goldstein, Abraham; Levy, Jonathan; Weisman, Omri; Schneiderman, Inna; Mankuta, David; Zagoory-Sharon, Orna; Feldman, Ruth

    2015-01-01

    Exposure to combat-related trauma often leads to lifetime functional impairments. Previous research demonstrated the effects of oxytocin (OT) administration on brain regions implicated in post-traumatic stress disorder (PTSD); yet OT’s effects on brain patterns in trauma-exposed veterans have not been studied. In the current study the effects of OT on spontaneous brain oscillatory activity were measured in 43 veterans using magnetoencephalography (MEG): 28 veterans who were exposed to a combat-related trauma and 15 trauma-unexposed controls. Participants participated in two experimental sessions and were administered OT or placebo (PBO) in a double-blind, placebo-control, within-subject design. Following OT/PBO administration, participants underwent a whole-head MEG scan. Plasma and salivary OT levels were assessed each session. Spontaneous brain activity measured during a 2-min resting period was subjected to source-localization analysis. Trauma-exposed veterans showed higher resting-state alpha (8–13 Hz) activity compared to controls in the left dorsolateral prefrontal cortex (dlPFC), specifically in the superior frontal gyrus (SFG) and the middle frontal gyrus (MFG), indicating decreased neural activity in these regions. The higher alpha activity was “normalized” following OT administration and under OT, group differences were no longer found. Increased resting-state alpha was associated with lower baseline plasma OT, reduced salivary OT reactivity, and more re-experiencing symptoms. These findings demonstrate effects of OT on resting-state brain functioning in prefrontal regions subserving working memory and cognitive control, which are disrupted in PTSD. Results raise the possibility that OT, traditionally studied in social contexts, may also enhance performance in cognitive tasks associated with working memory and cognitive control following trauma exposure. PMID:26175673

  1. Oxytocin increases willingness to socially share one's emotions.

    PubMed

    Lane, Anthony; Luminet, Olivier; Rimé, Bernard; Gross, James J; de Timary, Philippe; Mikolajczak, Moïra

    2013-01-01

    Oxytocin (OT) is a neuropeptide that is attracting growing attention from researchers interested in human emotional and social behavior. There is indeed increasing evidence that OT has a calming effect and that it facilitates pair-bonding and social interactions. Some of OT's effects are thought to be direct, but it has been suggested that OT also may have indirect effects, mediated by changes in behavior. One potentially relevant behavioral change is an increased propensity for "emotional sharing" as this behavior, like OT, is known to have both calming and bonding effects. In this study, 60 healthy young adult men were randomly assigned to receive either intranasal placebo (PL; n = 30) or oxytocin (OT; n = 30). Participants were then instructed to retrieve a painful memory. Subsequently, OT and placebo participants' willingness to disclose to another person event-related facts (factual sharing) vs. event-related emotions (emotional sharing) was evaluated. Whereas the two groups were equally willing to disclose event-related facts, oxytocin was found to specifically increase the willingness to share event-related emotions. This study provides the first evidence that OT increases people's willingness to share their emotions. Importantly, OT did not make people more talkative (word counts were comparable across the two groups) but instead increased the willingness to share the specific component that is responsible for the calming and bonding effects of social sharing: emotions. Findings suggest that OT may shape the form of social sharing so as to maximize its benefits. This might help explain the calming and bonding effects of OT.

  2. Oxytocin during the initial stages of romantic attachment: Relations to couples’ interactive reciprocity

    PubMed Central

    Schneiderman, Inna; Zagoory-Sharon, Orna; Leckman, James F.; Feldman, Ruth

    2014-01-01

    Summary Romantic relationships can have a profound effect on adults’ health and well-being whereas the inability to maintain intimate bonds has been associated with physical and emotional distress. Studies in monogamous mammalian species underscore the central role of oxytocin (OT) in pair-bonding and human imaging studies implicate OT-rich brain areas in early romantic love. To assess the role of OT in romantic attachment, we examined plasma OT in 163 young adults: 120 new lovers (60 couples) three months after the initiation of their romantic relationship and 43 non-attached singles. Twenty-five of the 36 couples who stayed together were seen again six months later. Couples were observed in dyadic interactions and were each interviewed regarding relationship-related thoughts and behaviors. OT was significantly higher in new lovers compared to singles, F(1, 152) = 109.33, p < .001, which may suggest increased activity of the oxytocinergic system during the early stages of romantic attachment. These high levels of OT among new lovers did not decrease six months later and showed high individual stability. OT correlated with the couples’ interactive reciprocity, including social focus, positive affect, affectionate touch, and synchronized dyadic states, and with anxieties and worries regarding the partner and the relationship, findings which parallel those described for parent–infant bonding. OT levels at the first assessment differentiated couples who stayed together six months later from those who separated during this period. Regression analysis showed that OT predicted interactive reciprocity independent of sex, relationship duration, and the partner’s OT. Findings suggest that OT may play an important role at the first stages of romantic attachment and lend support to evolutionary models suggesting that parental and romantic attachment share underlying bio-behavioral mechanisms. PMID:22281209

  3. Working in the Methodological "Outfield": The Case of Bourdieu and Occupational Therapy

    ERIC Educational Resources Information Center

    Watson, Jo; Grenfell, Michael

    2016-01-01

    The article reports on a study of methodological innovation involving occupational therapy (OT) students in higher education (HE). It is based on an original project which examined the experiences and outcomes of non-traditional entrants to pre-registration OT education. A feature of the original project was the application of the epistemological…

  4. Oxytocin Signaling in Basolateral and Central Amygdala Nuclei Differentially Regulates the Acquisition, Expression, and Extinction of Context-Conditioned Fear in Rats

    ERIC Educational Resources Information Center

    Campbell-Smith, Emma J.; Holmes, Nathan M.; Lingawi, Nura W.; Panayi, Marios C.; Westbrook, R. Frederick

    2015-01-01

    The present study investigated how oxytocin (OT) signaling in the central (CeA) and basolateral (BLA) amygdala affects acquisition, expression, and extinction of context-conditioned fear (freezing) in rats. In the first set of experiments, acquisition of fear to a shocked context was impaired by a preconditioning infusion of synthetic OT into the…

  5. Effects of Online Translation on Morphosyntactic and Lexical-Pragmatic Accuracy in Essay Writing in Spanish as a Foreign Language

    ERIC Educational Resources Information Center

    Fredholm, Kent

    2014-01-01

    The use of online translation (OT) is increasing as more pupils receive laptops from their schools. This study investigates OT use in two groups of Swedish pupils (ages 17-18) studying Spanish as an L3: one group (A) having free Internet access and the spelling and grammar checker of Microsoft Word, the other group (B) using printed dictionaries…

  6. Instructional Strategies Used to Improve Students' Comfort and Skill in Addressing the Occupational Therapy Process

    ERIC Educational Resources Information Center

    Knecht-Sabres, Lisa Jean; Egan, Brad E.; Wallingford, Minetta S.; Kovic, Mark

    2015-01-01

    The purpose of this study was to investigate the effectiveness of an intentional blending of instructional strategies in an occupational therapy (OT) entry-level master's course. The OT Adult Practice course uses case-based instructional strategies, clinical skills labs, and standardized patient experiences in a dovetailed approach across three…

  7. Molecular evolution of the neurohypophysial hormone precursors in mammals: Comparative genomics reveals novel mammalian oxytocin and vasopressin analogues.

    PubMed

    Wallis, Michael

    2012-11-01

    Among vertebrates the neurohypophysial hormones show considerable variation. However, in eutherian mammals they have been considered rather conserved, with arginine vasopressin (AVP) and oxytocin (OT) in all species except pig and some relatives, where lysine vasopressin replaces AVP. The availability of genomic data for a wide range of mammals makes it possible to assess whether these peptides and their precursors may be more variable in Eutheria than previously suspected. A survey of these data confirms that AVP and OT occur in most eutherians, but with exceptions. In a New-World monkey (marmoset, Callithrix jacchus) and in tree shrew (Tupaia belangeri), Pro(8)OT replaces OT, confirming a recent report for these species. In armadillo (Dasypus novemcinctus) Leu(3)OT replaces OT, while in tenrec (Echinops telfairi) Thr(4)AVP replaces AVP. In these two species there is also evidence for additional genes/pseudogenes, encoding much-modified forms of AVP, but in most other eutherian species there is no evidence for additional neurohypophysial hormone genes. Evolutionary analysis shows that sequences of eutherian neurohypophysial hormone precursors are generally strongly conserved, particularly those regions encoding active peptide and neurophysin. The close association between OT and VP genes has led to frequent gene conversion of sequences encoding neurophysins. A monotreme, platypus (Ornithorhynchus anatinus) has genes for OT and AVP, organized tail-to-tail as in eutherians, but in marsupials 3-4 genes are present for neurohypophysial hormones, organized tail-to-head as in lower vertebrates.

  8. Association between intermediate uveitis and toxocariasis in the Korean population

    PubMed Central

    Kwon, Jin-woo; Sim, Yoonseob; Jee, Donghyun

    2017-01-01

    Abstract The aim of the study was to investigate the characteristics of ocular toxocariasis (OT) presenting with intermediate uveitis in the Korean population. We studied intermediate uveitis patients using ocular and systemic evaluations and a Toxocara IgG serology test. Of 50 intermediate uveitis patients, 19 were seropositive for Toxocara IgG. Of the 19 OT patients, 4 presented with recurrence within 6 months and were significantly younger than nonrecurrence patients (P = 0.009). Thirteen patients had a history of eating raw cow liver. There were 14 males and 5 females in the OT group, and 11 males and 20 females in the non-OT group (P = 0.009). There was bilateral involvement in 7 out of 19 patients in the OT group, and 20 out of 31 patients in non-OT group (P = 0.033). Intermediate uveitis patients in OT were predominately male and had more unilateral presentation than non-OT patients. PMID:28151857

  9. Reading Without the Left Ventral Occipito-Temporal Cortex

    ERIC Educational Resources Information Center

    Seghier, Mohamed L.; Neufeld, Nicholas H.; Zeidman, Peter; Leff, Alex P.; Mechelli, Andrea; Nagendran, Arjuna; Riddoch, Jane M.; Humphreys, Glyn W.; Price, Cathy J.

    2012-01-01

    The left ventral occipito-temporal cortex (LvOT) is thought to be essential for the rapid parallel letter processing that is required for skilled reading. Here we investigate whether rapid written word identification in skilled readers can be supported by neural pathways that do not involve LvOT. Hypotheses were derived from a stroke patient who…

  10. Dehydration anorexia is attenuated in oxytocin-deficient mice.

    PubMed

    Rinaman, Linda; Vollmer, Regis R; Karam, Joseph; Phillips, Donnesha; Li, Xia; Amico, Janet A

    2005-06-01

    Evidence in rats suggests that central oxytocin (OT) signaling pathways contribute to suppression of food intake during dehydration (i.e., dehydration anorexia). The present study examined water deprivation-induced dehydration anorexia in wild-type and OT -/- mice. Mice were deprived of food alone (fasted, euhydrated) or were deprived of both food and water (fasted, dehydrated) for 18 h overnight. Fasted wild-type mice consumed significantly less chow during a 60-min refeeding period when dehydrated compared with their intake when euhydrated. Conversely, fasting-induced food intake was slightly but not significantly suppressed by dehydration in OT -/- mice, evidence for attenuated dehydration anorexia. In a separate experiment, mice were deprived of water (but not food) overnight for 18 h; then they were anesthetized and perfused with fixative for immunocytochemical analysis of central Fos expression. Fos was elevated similarly in osmo- and volume-sensitive regions of the basal forebrain and hypothalamus in wild-type and OT -/- mice after water deprivation. OT-positive neurons expressed Fos in dehydrated wild-type mice, and vasopressin-positive neurons were activated to a similar extent in wild-type and OT -/- mice. Conversely, significantly fewer neurons within the hindbrain dorsal vagal complex were activated in OT -/- mice after water deprivation compared with activation in wild-type mice. These findings support the view that OT-containing projections from the hypothalamus to the hindbrain are necessary for the full expression of compensatory behavioral and physiological responses to dehydration.

  11. Adsorption of methylene blue on an agro-waste oiltea shell with and without fungal treatment

    NASA Astrophysics Data System (ADS)

    Liu, Jiayang; Li, Enzhong; You, Xiaojuan; Hu, Changwei; Huang, Qingguo

    2016-12-01

    A lignocellulosic waste oiltea shell (OTS) was evaluated as an inexpensive sorbent to remove methylene blue (MB) from aqueous solution. Fungal treatment of OTS increased the MB adsorption by modifying the physicochemical properties of OTS and simultaneously produced laccase as a beneficial co-product. Without fungal treatment, the maximum amount of adsorption (qm) of MB by OTS was 64.4 mg/g, whereas the treatment with fungus Pycnoporus sp. and Trametes versicolor increased qm up to 72.5 mg/g and 85.7 mg/g, respectively. This is because of the improved surface area and pore sizes as well as altered chemical compositions. The equilibrium sorption data for OTS both with and without treatment fitted to the Langmuir model, and the sorption rate data well fitted to the pseudo second-order kinetic model. The changes in free energy (ΔG°) and separation factor (RL) indicated that the sorption was spontaneous and favorable. Scanning electron microscopy and Fourier transform infrared spectroscopy showed the changes in the surface morphology and functional groups of OTS after fungal treatment. The agro-waste OTS could be utilized as a low-cost adsorbent for efficient dye removal, and fungal treatment can serve as a mild and clean technique to increase the adsorptive capacity of OTS.

  12. Using probabilistic modeling to evaluate human exposure to organotin in drinking water transported by polyvinyl chloride pipe.

    PubMed

    Fristachi, Anthony; Xu, Ying; Rice, Glenn; Impellitteri, Christopher A; Carlson-Lynch, Heather; Little, John C

    2009-11-01

    The leaching of organotin (OT) heat stabilizers from polyvinyl chloride (PVC) pipes used in residential drinking water systems may affect the quality of drinking water. These OTs, principally mono- and di-substituted species of butyltins and methyltins, are a potential health concern because they belong to a broad class of compounds that may be immune, nervous, and reproductive system toxicants. In this article, we develop probability distributions of U.S. population exposures to mixtures of OTs encountered in drinking water transported by PVC pipes. We employed a family of mathematical models to estimate OT leaching rates from PVC pipe as a function of both surface area and time. We then integrated the distribution of estimated leaching rates into an exposure model that estimated the probability distribution of OT concentrations in tap waters and the resulting potential human OT exposures via tap water consumption. Our study results suggest that human OT exposures through tap water consumption are likely to be considerably lower than the World Health Organization (WHO) "safe" long-term concentration in drinking water (150 microg/L) for dibutyltin (DBT)--the most toxic of the OT considered in this article. The 90th percentile average daily dose (ADD) estimate of 0.034 +/- 2.92 x 10(-4)microg/kg day is approximately 120 times lower than the WHO-based ADD for DBT (4.2 microg/kg day).

  13. The oxytocin system in drug discovery for autism: Animal models and novel therapeutic strategies

    PubMed Central

    Modi, Meera E.; Young, Larry J.

    2012-01-01

    Animal models and behavioral paradigms are critical for elucidating the neural mechanism involved in complex behaviors, including social cognition. Both genotype and phenotype based models have implicated the neuropeptide oxytocin (OT) in the regulation of social behavior. Based on the findings in animal models, alteration of the OT system has been hypothesized to play a role in the social deficits associated with autism and other neuropsychiatric disorders. While the evidence linking the peptide to the etiology of the disorder is not yet conclusive, evidence from multiple animal models suggest modulation of the OT system may be a viable strategy for the pharmacological treatment of social deficits. In this review, we will discuss how animal models have been utilized to understand the role of OT in social cognition and how those findings can be applied to the conceptualization and treatment of the social impairments in ASD. Animal models with genetic alterations of the OT system, like the OT, OT receptor and CD38 knock-out mice, and those with phenotypic variation in social behavior, like BTBR inbred mice and prairie voles, coupled with behavioral paradigms with face and construct validity may prove to have predictive validity for identifying the most efficacious methods of stimulating the OT system to enhance social cognition in humans. The widespread use of strong animal models of social cognition has the potential yield pharmacological, interventions for the treatment social impairments psychiatric disorders. This article is part of a Special Issue entitled Oxytocin, Vasopressin, and Social Behavior. PMID:22206823

  14. 76 FR 28504 - Lending and Investment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... Office of Thrift Supervision Lending and Investment AGENCY: Office of Thrift Supervision (OTS), Treasury... collection. Title of Proposal: Lending and Investment. OMB Number: 1550-0078. Form Number: N/A. Description: Current OTS regulations for the documentation of loans and investments for safety and soundness...

  15. 12 CFR 4.37 - Persons and entities with access to OCC information; prohibition on dissemination.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... § 4.37 Persons and entities with access to OCC information; prohibition on dissemination. (a) Current... by this subpart or otherwise by the OCC, no current or or former OTS employee or agent may, in any.... Any current or or former OTS employee or agent subpoenaed or otherwise requested to...

  16. Possible Orphan Transient Detection at PGC143111 galaxy by MASTER

    NASA Astrophysics Data System (ADS)

    Popova, E.; Lipunov, V.; Gorbovskoy, E.; Lopez, R. Rebolo; Serra-Ricart, M.; Israelian, G.; Lodieu, N.; Suarez-Andres, L.; Kornilov, V.; Tiurina, N.; Balanutsa, P.; Samus, N.; Gress, O.; Budnev, N.; Ivanov, K.; Buckley, D.; Potter, S.; Knyazev, A.; Kotze, M.; Tlatov, A.; Senik, V.; Dormidontov, D.; Krushinskiy, V.; Sergienko, Yu.; Gabovich, A.; Yurkov, V.

    2015-10-01

    MASTER-IAC auto-detection system ( Lipunov et al., Advances in Astronomy, MASTER Global Robotic Net, 2010 ) discovered OT source at (RA, Dec) = 00h 00m 08.72s -33d 41m 56.5s on 2015-10-13.98017 UT. The OT unfiltered magnitude is 17.6m (the limit is 19.9m).

  17. A New Observing Tool for the James Clerk Maxwell Telescope

    NASA Astrophysics Data System (ADS)

    Folger, Martin; Bridger, Alan; Dent, Bill; Kelly, Dennis; Adamson, Andy; Economou, Frossie; Hirst, Paul; Jenness, Tim

    A new Observing Tool (OT) has been developed at the UK Astronomy Technology Centre, Edinburgh, UK and the Joint Astronomy Centre, Hilo, Hawaii, USA. It is based on the Gemini Observing Tool and provides the first graphical observation preparation tool for the James Clerk Maxwell Telescope (JCMT) as well as being the first use of the OT for a non-optical/IR telescope. The OT allows the observer to assemble high level Science Programs using graphical representations of observation components such as instrument, target, and filter. This is later translated into low level control sequences for telescope and instruments. The new OT is designed to work on multiple telescopes: currently the UK Infrared Telescope (UKIRT) and JCMT. Object-oriented design makes the inclusion of telescope and instrument specific packages easy. The OT is written in Java using GUI packages such as Swing and JSky. A new component for the JCMT OT is the graphical Frequency Editor for Heterodyne instruments. It can be used to specify parameters such as frequencies, bandwidths, and sidebands of multiple subsystems, while graphically displaying the front-end frequency, emission lines and atmospheric transmission. In addition, Flexible Scheduling support has been added to the OT. The observer can define scheduling constraints by arranging observations graphically. Science Programs can be saved as XML or sent directly from the OT to a database (via SOAP).

  18. MASTER: PSN in bright spiral PGC2120251

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Tlatov, A.; Senik, V.; Dormidontov, D.; Parkhomenko, A.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Vladimirov, V.; Gress, O.; Shumkov, V.; Kuvshinov, D.; Gabovich, A.; Yurkov, V.; Pogrosheva, T.

    2017-01-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 08h 15m 06.13s +38d 11m 23.3s on 2017-01-16.01742 UT. The OT unfiltered magnitude is (limit 18.6m).

  19. MASTER-IAC: 2 optical transients

    NASA Astrophysics Data System (ADS)

    Gress, O.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Shumkov, V.; Vladimirov, V.; Pogrosheva, T.

    2017-01-01

    MASTER-IAC auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 41m 21.42s +34d 16m 46.7s on 2017-01-26.25294 UT. The OT unfiltered magnitude is 17.0m (limit 18.9m).

  20. 5 CFR 3101.109 - Additional rules for Office of Thrift Supervision employees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... employee. For purposes of this section, the term “covered OTS employee” means: (1) An OTS examiner; (2) An...) Definition of “securities”. For purposes of paragraphs (b)(1) and (b)(3) of this section, the term “securities” includes all interests in debt or equity instruments. The term includes, without...