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Sample records for pot1 avoids rna

  1. Two RNA subunits and POT1a are components of Arabidopsis telomerase

    PubMed Central

    Cifuentes-Rojas, Catherine; Kannan, Kalpana; Tseng, Lin; Shippen, Dorothy E.

    2011-01-01

    Telomerase is a ribonucleoprotein (RNP) reverse transcriptase whose essential RNA subunit (TER) functions as a template for telomere repeat synthesis. Here we report the identification of two divergent TER moieties in the flowering plant Arabidopsis thaliana. Although both TER1 and TER2 copurify with telomerase activity and serve as templates for telomerase in vitro, depletion of TER1, but not TER2, leads to decreased telomerase activity and progressive telomere shortening in vivo. Moreover, mutation of the templating domain in TER1 results in the incorporation of mutant telomere repeats on chromosome ends. Thus, TER1 provides the major template for telomerase in vivo. We also show that POT1a binds TER1 with a Kd of 2 × 10-7 M and the two components assemble into an enzymatically active RNP in vivo. In contrast, TER1-POT1b and TER2-POT1a associations were not observed. In other organisms POT1 proteins bind telomeric DNA and provide chromosome end protection. We propose that duplication of TER and POT1 in Arabidopsis fueled the evolution of novel protein–nucleic acid interactions and the migration of POT1 from the telomere to the telomerase RNP. PMID:21164032

  2. POT1 and TRF2 Cooperate To Maintain Telomeric Integrity†

    PubMed Central

    Yang, Qin; Zheng, Yun-Ling; Harris, Curtis C.

    2005-01-01

    Mammalian telomeric DNA contains duplex TTAGGG repeats and single-stranded overhangs. POT1 (protection of telomeres 1) is a telomere-specific single-stranded DNA-binding protein, highly conserved in eukaryotes. The biological function of human POT1 is not well understood. In the present study, we demonstrate that POT1 plays a key role in telomeric end protection. The reduction of POT1 by RNA interference led to the loss of telomeric single-stranded overhangs and induced apoptosis, chromosomal instability, and senescence in cells. POT1 and TRF2 interacted with each other to form a complex with telomeric DNA. A dominant negative TRF2, TRF2ΔBΔM, bound to POT1 and prevented it from binding to telomeres. POT1 overexpression protected against TRF2ΔBΔM-induced loss of telomeric single-stranded overhangs, chromosomal instability, and senescence. These results demonstrate that POT1 and TRF2 share in part in the same pathway for telomere capping and suggest that POT1 binds to the telomeric single-stranded DNA in the D-loop and cooperates with TRF2 in t-loop maintenance. PMID:15657433

  3. Pot1 and Telomere Maintenance

    PubMed Central

    Baumann, Peter; Price, Carolyn

    2010-01-01

    Proteins that specifically bind the single stranded overhang at the ends of telomeres have been identified in a wide range of eukaryotes and play pivotal roles in chromosome end protection and telomere length regulation. Here we summarize recent findings regarding the functions of POT1 proteins in vertebrates and discuss the functional evolution of POT1 proteins following gene duplication in protozoa, plants, nematodes and mice. PMID:20493859

  4. DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe

    PubMed Central

    Nandakumar, Jayakrishnan; Cech, Thomas R.

    2012-01-01

    Eukaryotic chromosome ends are protected from illicit DNA joining by protein–DNA complexes called telomeres. In most studied organisms, telomeric DNA is composed of multiple short G-rich repeats that end in a single-stranded tail that is protected by the protein POT1. Mammalian POT1 binds two telomeric repeats as a monomer in a sequence-specific manner, and discriminates against RNA of telomeric sequence. While addressing the RNA discrimination properties of SpPot1, the POT1 homolog in Schizosaccharomyces pombe, we found an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats. DNA binding seems to be achieved via binding of the most N-terminal OB domain of each monomer to each telomeric repeat. The SpPot1 dimer may have evolved to accommodate the heterogeneous spacers that occur between S. pombe telomeric repeats, and it also has implications for telomere architecture. We further show that the S. pombe telomeric protein Tpz1, like its mammalian homolog TPP1, increases the affinity of Pot1 for telomeric single-stranded DNA and enhances the discrimination of Pot1 against RNA. PMID:21911358

  5. Structural insights into POT1-TPP1 interaction and POT1 C-terminal mutations in human cancer.

    PubMed

    Chen, Cong; Gu, Peili; Wu, Jian; Chen, Xianyun; Niu, Shuangshuang; Sun, Hong; Wu, Lijie; Li, Na; Peng, Junhui; Shi, Shaohua; Fan, Cuiying; Huang, Min; Wong, Catherine C L; Gong, Qingguo; Kumar-Sinha, Chandan; Zhang, Rongguang; Pusztai, Lajos; Rai, Rekha; Chang, Sandy; Lei, Ming

    2017-04-10

    Mammalian shelterin proteins POT1 and TPP1 form a stable heterodimer that protects chromosome ends and regulates telomerase-mediated telomere extension. However, how POT1 interacts with TPP1 remains unknown. Here we present the crystal structure of the C-terminal portion of human POT1 (POT1C) complexed with the POT1-binding motif of TPP1. The structure shows that POT1C contains two domains, a third OB fold and a Holliday junction resolvase-like domain. Both domains are essential for binding to TPP1. Notably, unlike the heart-shaped structure of ciliated protozoan Oxytricha nova TEBPα-β complex, POT1-TPP1 adopts an elongated V-shaped conformation. In addition, we identify several missense mutations in human cancers that disrupt the POT1C-TPP1 interaction, resulting in POT1 instability. POT1C mutants that bind TPP1 localize to telomeres but fail to repress a DNA damage response and inappropriate repair by A-NHEJ. Our results reveal that POT1 C terminus is essential to prevent initiation of genome instability permissive for tumorigenesis.

  6. TPP1 is a homologue of ciliate TEBP-β and interacts with POT1 to recruit telomerase

    NASA Astrophysics Data System (ADS)

    Xin, Huawei; Liu, Dan; Wan, Ma; Safari, Amin; Kim, Hyeung; Sun, Wen; O'Connor, Matthew S.; Songyang, Zhou

    2007-02-01

    Telomere dysfunction may result in chromosomal abnormalities, DNA damage responses, and even cancer. Early studies in lower organisms have helped to establish the crucial role of telomerase and telomeric proteins in maintaining telomere length and protecting telomere ends. In Oxytricha nova, telomere G-overhangs are protected by the TEBP-α/β heterodimer. Human telomeres contain duplex telomeric repeats with 3' single-stranded G-overhangs, and may fold into a t-loop structure that helps to shield them from being recognized as DNA breaks. Additionally, the TEBP-α homologue, POT1, which binds telomeric single-stranded DNA (ssDNA), associates with multiple telomeric proteins (for example, TPP1, TIN2, TRF1, TRF2 and RAP1) to form the six-protein telosome/shelterin and other subcomplexes. These telomeric protein complexes in turn interact with diverse pathways to form the telomere interactome for telomere maintenance. However, the mechanisms by which the POT1-containing telosome communicates with telomerase to regulate telomeres remain to be elucidated. Here we demonstrate that TPP1 is a putative mammalian homologue of TEBP-β and contains a predicted amino-terminal oligonucleotide/oligosaccharide binding (OB) fold. TPP1-POT1 association enhanced POT1 affinity for telomeric ssDNA. In addition, the TPP1 OB fold, as well as POT1-TPP1 binding, seemed critical for POT1-mediated telomere-length control and telomere-end protection in human cells. Disruption of POT1-TPP1 interaction by dominant negative TPP1 expression or RNA interference (RNAi) resulted in telomere-length alteration and DNA damage responses. Furthermore, we offer evidence that TPP1 associates with the telomerase in a TPP1-OB-fold-dependent manner, providing a physical link between telomerase and the telosome/shelterin complex. Our findings highlight the critical role of TPP1 in telomere maintenance, and support a yin-yang model in which TPP1 and POT1 function as a unit to protect human telomeres, by both

  7. Avoidance of stochastic RNA interactions can be harnessed to control protein expression levels in bacteria and archaea

    PubMed Central

    Umu, Sinan Uğur; Poole, Anthony M; Dobson, Renwick CJ; Gardner, Paul P

    2016-01-01

    A critical assumption of gene expression analysis is that mRNA abundances broadly correlate with protein abundance, but these two are often imperfectly correlated. Some of the discrepancy can be accounted for by two important mRNA features: codon usage and mRNA secondary structure. We present a new global factor, called mRNA:ncRNA avoidance, and provide evidence that avoidance increases translational efficiency. We also demonstrate a strong selection for the avoidance of stochastic mRNA:ncRNA interactions across prokaryotes, and that these have a greater impact on protein abundance than mRNA structure or codon usage. By generating synonymously variant green fluorescent protein (GFP) mRNAs with different potential for mRNA:ncRNA interactions, we demonstrate that GFP levels correlate well with interaction avoidance. Therefore, taking stochastic mRNA:ncRNA interactions into account enables precise modulation of protein abundance. DOI: http://dx.doi.org/10.7554/eLife.13479.001 PMID:27642845

  8. A Small Molecule Inhibitor of Pot1 Binding to Telomeric DNA

    PubMed Central

    Altschuler, Sarah E.; Croy, Johnny E.; Wuttke, Deborah S.

    2012-01-01

    Chromosome ends are complex structures, consisting of repetitive DNA sequence terminating in an ssDNA overhang with many associated proteins. Because alteration of these ends is a hallmark of cancer, telomeres and telomere maintenance have been prime drug targets. The universally conserved ssDNA overhang is sequence-specifically bound and regulated by Pot1 (protection of telomeres), and perturbation of Pot1 function has deleterious effects for proliferating cells. The specificity of the Pot1/ssDNA interaction and the key involvement of this protein in telomere maintenance have suggested directed inhibition of Pot1/ssDNA binding as an efficient means of disrupting telomere function. To explore this idea, we developed a high-throughput time-resolved fluorescence resonance energy transfer (TR-FRET) screen for inhibitors of Pot1/ssDNA interaction. We conducted this screen with the DNA-binding subdomain of S. pombe Pot1 (Pot1pN), which confers the vast majority of Pot1 sequence-specificity and is highly similar to the first domain of human Pot1 (hPOT1). Screening a library of ~20,000 compounds yielded a single inhibitor, which we found interacted tightly with submicromolar affinity. Furthermore, this compound, subsequently identified as the bis-azo dye Congo red, was able to competitively inhibit hPOT1 binding to telomeric DNA. ITC and NMR chemical shift analysis suggest that CR interacts specifically with the ssDNA-binding cleft of Pot1, and that alteration of this surface disrupts CR binding. The identification of a specific inhibitor of ssDNA interaction establishes a new pathway for targeted telomere disruption. PMID:22978652

  9. Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation.

    PubMed

    Takahashi, Katsunori; Imano, Ryota; Kibe, Tatsuya; Seimiya, Hiroyuki; Muramatsu, Yukiko; Kawabata, Naoki; Tanaka, Genki; Matsumoto, Yoshitake; Hiromoto, Taisuke; Koizumi, Yuka; Nakazawa, Norihiko; Yanagida, Mitsuhiro; Yukawa, Masashi; Tsuchiya, Eiko; Ueno, Masaru

    2011-02-01

    Pot1 is a single-stranded telomere-binding protein that is conserved from fission yeast to mammals. Deletion of Schizosaccharomyces pombe pot1(+) causes immediate telomere loss. S. pombe Rqh1 is a homolog of the human RecQ helicase WRN, which plays essential roles in the maintenance of genomic stability. Here, we demonstrate that a pot1Δ rqh1-hd (helicase-dead) double mutant maintains telomeres that are dependent on Rad51-mediated homologous recombination. Interestingly, the pot1Δ rqh1-hd double mutant displays a "cut" (cell untimely torn) phenotype and is sensitive to the antimicrotubule drug thiabendazole (TBZ). Moreover, the chromosome ends of the double mutant do not enter the pulsed-field electrophoresis gel. These results suggest that the entangled chromosome ends in the pot1Δ rqh1-hd double mutant inhibit chromosome segregation, signifying that Pot1 and Rqh1 are required for efficient chromosome segregation. We also found that POT1 knockdown, WRN-deficient human cells are sensitive to the antimicrotubule drug vinblastine, implying that some of the functions of S. pombe Pot1 and Rqh1 may be conserved in their respective human counterparts POT1 and WRN.

  10. Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA.

    PubMed

    Glushakova, Lyudmyla G; Sharma, Nidhi; Hoshika, Shuichi; Bradley, Andrea C; Bradley, Kevin M; Yang, Zunyi; Benner, Steven A

    2015-11-15

    Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. Although these all have rapid turnaround, they are expensive to run. Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false positives, and false negatives; these arise by interactions between the multiple nucleic acid primers and probes in a multiplexed kit. Here we offer a multiplexed assay for a panel of respiratory viruses that mitigates these problems by combining several nucleic acid analogs from the emerging field of synthetic biology: (i) self-avoiding molecular recognition systems (SAMRSs), which facilitate multiplexing, and (ii) artificially expanded genetic information systems (AEGISs), which enable low-noise PCR. These are supplemented by "transliteration" technology, which converts standard nucleotides in a target to AEGIS nucleotides in a product, improving hybridization. The combination supports a multiplexed Luminex-based respiratory panel that potentially differentiates influenza viruses A and B, respiratory syncytial virus, severe acute respiratory syndrome coronavirus (SARS), and Middle East respiratory syndrome (MERS) coronavirus, detecting as few as 10 MERS virions in a 20-μl sample.

  11. Interaction of Berberine derivative with protein POT1 affect telomere function in cancer cells

    SciTech Connect

    Xiao, Nannan; Chen, Siqi; Ma, Yan; Qiu, Jun; Tan, Jia-Heng; Ou, Tian-Miao; Gu, Lian-Quan; Huang, Zhi-Shu; Li, Ding

    2012-03-16

    Highlights: Black-Right-Pointing-Pointer The protein POT1 plays an important role in telomere protection. Black-Right-Pointing-Pointer Functional POT1 was overexpressed in Escherichia coli for the first time, and purified. Black-Right-Pointing-Pointer Compound Sysu-00692 was found to be the first POT1-binding ligand. Black-Right-Pointing-Pointer Sysu-00692 could interfere with the binding activity of POT1 in vivo. Black-Right-Pointing-Pointer Sysu-00692 had inhibition on telomerase and cell proliferation. -- Abstract: The protein POT1 plays an important role in telomere protection, which is related with telomere elongation and cell immortality. The protein has been recognized as a promising drug target for cancer treatment. In the present study, we cloned, overexpressed in Escherichia coli for the first time, and purified recombinant human POT1. The protein was proved to be active through filter binding assay, FRET and CD experiments. In the initial screening for protein binding ligands using SPR, compound Sysu-00692 was found to bind well with the POT1, which was confirmed with EMSA. Its in vivo activity study showed that compound Sysu-00692 could interfere with the binding between human POT1 and the telomeric DNA through chromatin immunoprecipitation. Besides, the compound showed mild inhibition on telomerase and cell proliferation. As we know, compound Sysu-00692 is the first reported POT1-binding ligand, which could serve as a lead compound for further improvement. This work offered a potentially new approach for drug design for the treatment of cancers.

  12. The protein network surrounding the human telomere repeat binding factors TRF1, TRF2, and POT1

    SciTech Connect

    Giannone, Richard J; McDonald, W Hayes; Hurst, Gregory {Greg} B; Shen, Rong-Fong; Wang, Yisong; Liu, Yie

    2010-01-01

    Telomere integrity (including telomere length and capping) is critical in overall genomic stability. Telomere repeat binding factors and their associated proteins play vital roles in telomere length regulation and end protection. In this study, we explore the protein network surrounding telomere repeat binding factors, TRF1, TRF2, and POT1 using dual-tag affinity purification in combination with multidimensional protein identification technology liquid chromatography - tandem mass spectrometry (MudPIT LC-MS/MS). After control subtraction and data filtering, we found that TRF2 and POT1 co-purified all six members of the telomere protein complex, while TRF1 identified five of six components at frequencies that lend evidence towards the currently accepted telomere architecture. Many of the known TRF1 or TRF2 interacting proteins were also identified. Moreover, putative associating partners identified for each of the three core components fell into functional categories such as DNA damage repair, ubiquitination, chromosome cohesion, chromatin modification/remodeling, DNA replication, cell cycle and transcription regulation, nucleotide metabolism, RNA processing, and nuclear transport. These putative protein-protein associations may participate in different biological processes at telomeres or, intriguingly, outside telomeres.

  13. POT1 stimulates RecQ helicases WRN and BLM to unwind telomeric DNA substrates.

    PubMed

    Opresko, Patricia L; Mason, Penelope A; Podell, Elaine R; Lei, Ming; Hickson, Ian D; Cech, Thomas R; Bohr, Vilhelm A

    2005-09-16

    Defects in human RecQ helicases WRN and BLM are responsible for the cancer-prone disorders Werner syndrome and Bloom syndrome. Cellular phenotypes of Werner syndrome and Bloom syndrome, including genomic instability and premature senescence, are consistent with telomere dysfunction. RecQ helicases are proposed to function in dissociating alternative DNA structures during recombination and/or replication at telomeric ends. Here we report that the telomeric single-strand DNA-binding protein, POT1, strongly stimulates WRN and BLM to unwind long telomeric forked duplexes and D-loop structures that are otherwise poor substrates for these helicases. This stimulation is dependent on the presence of telomeric sequence in the duplex regions of the substrates. In contrast, POT1 failed to stimulate a bacterial 3'-5'-helicase. We find that purified POT1 binds to WRN and BLM in vitro and that full-length POT1 (splice variant 1) precipitates a higher amount of endogenous WRN protein, compared with BLM, from the HeLa nuclear extract. We propose roles for the cooperation of POT1 with RecQ helicases WRN and BLM in resolving DNA structures at telomeric ends, in a manner that protects the telomeric 3' tail as it is exposed during unwinding.

  14. Nonadditivity in the recognition of single-stranded DNA by the schizosaccharomyces pombe protection of telomeres 1 DNA-binding domain, Pot1-DBD.

    PubMed

    Croy, Johnny E; Altschuler, Sarah E; Grimm, Nicole E; Wuttke, Deborah S

    2009-07-28

    The Schizosaccharomyces pombe protection of telomeres 1 (SpPot1) protein recognizes the 3' single-stranded ends of telomeres and provides essential protective and regulatory functions. The ssDNA-binding activity of SpPot1 is conferred by its ssDNA-binding domain, Pot1-DBD (residues 1-389), which can be further separated into two distinct domains, Pot1pN (residues 1-187) and Pot1pC (residues 188-389). Here we show that Pot1pC, like Pot1pN, can function independently of Pot1-DBD and binds specifically to a minimal nonameric oligonucleotide, d(GGTTACGGT), with a K(D) of 400 +/- 70 nM (specifically recognized nucleotides in bold). NMR chemical shift perturbation analysis indicates that the overall structures of the isolated Pot1pN and Pot1pC domains remain intact in Pot1-DBD. Furthermore, alanine scanning reveals modest differences in the ssDNA-binding contacts provided by isolated Pot1pN and within Pot1-DBD. Although the global character of both Pot1pN and Pot1pC is maintained in Pot1-DBD, chemical shift perturbation analysis highlights localized structural differences within the G1/G2 and T3/T4 binding pockets of Pot1pN in Pot1-DBD, which correlate with its distinct ssDNA-binding activity. Furthermore, we find evidence for a putative interdomain interface on Pot1pN that mediates interactions with Pot1pC that ultimately result in the altered ssDNA-binding activity of Pot1-DBD. Together, these data provide insight into the mechanisms underlying the activity and regulation of SpPot1 at the telomere.

  15. A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus.

    PubMed

    Takai, Hiroyuki; Jenkinson, Emma; Kabir, Shaheen; Babul-Hirji, Riyana; Najm-Tehrani, Nasrin; Chitayat, David A; Crow, Yanick J; de Lange, Titia

    2016-04-01

    Coats plus (CP) can be caused by mutations in the CTC1 component of CST, which promotes polymerase α (polα)/primase-dependent fill-in throughout the genome and at telomeres. The cellular pathology relating to CP has not been established. We identified a homozygous POT1 S322L substitution (POT1(CP)) in two siblings with CP. POT1(CP)induced a proliferative arrest that could be bypassed by telomerase. POT1(CP)was expressed at normal levels, bound TPP1 and telomeres, and blocked ATR signaling. POT1(CP)was defective in regulating telomerase, leading to telomere elongation rather than the telomere shortening observed in other telomeropathies. POT1(CP)was also defective in the maintenance of the telomeric C strand, causing extended 3' overhangs and stochastic telomere truncations that could be healed by telomerase. Consistent with shortening of the telomeric C strand, metaphase chromosomes showed loss of telomeres synthesized by leading strand DNA synthesis. We propose that CP is caused by a defect in POT1/CST-dependent telomere fill-in. We further propose that deficiency in the fill-in step generates truncated telomeres that halt proliferation in cells lacking telomerase, whereas, in tissues expressing telomerase (e.g., bone marrow), the truncations are healed. The proposed etiology can explain why CP presents with features distinct from those associated with telomerase defects (e.g., dyskeratosis congenita).

  16. The OB-fold domain 1 of human POT1 recognizes both telomeric and non-telomeric DNA motifs

    PubMed Central

    Kolar, Carol; Yan, Ying; Borgstahl, Gloria E.O.; Ouellette, Michel M.

    2015-01-01

    The POT1 protein plays a critical role in telomere protection and telomerase regulation. POT1 binds single-stranded 5’-TTAGGGTTAG-3’ and forms a dimer with the TPP1 protein. The dimer is recruited to telomeres, either directly or as part of the Shelterin complex. Human POT1 contains two Oligonucleotide/Oligosaccharide Binding (OB) fold domains, OB1 and OB2, which make physical contact with the DNA. OB1 recognizes 5’-TTAGGG whereas OB2 binds to the downstream TTAG-3’. Studies of POT1 proteins from other species have shown that some of these proteins are able to recognize a broader variety of DNA ligands than expected. To explore this possibility in humans, we have used SELEX to reexamine the sequence-specificity of the protein. Using human POT1 as a selection matrix, high-affinity DNA ligands were selected from a pool of randomized single-stranded oligonucleotides. After six successive rounds of selection, two classes of high-affinity targets were obtained. The first class was composed of oligonucleotides containing a cognate POT1 binding sites (5’-TTAGGGTTAG-3’). The second and more abundant class was made of molecules that carried a novel non-telomeric consensus: 5’-TNCANNAGKKKTTAGG-3’ (where K=G/T and N=any base). Binding studies showed that these non-telomeric sites were made of an OB1-binding motif (TTAGG) and a non-telomeric motif (NT motif), with the two motifs recognized by distinct regions of the OB1 domain. POT1 interacted with these non-telomeric binding sites with high affinity and specificity, even when bound to its dimerization partner TPP1. This intrinsic ability of POT1 to recognize NT motifs raises the possibility that the protein may fulfill additional functions at certain non-telomeric locations of the genome, in perhaps gene transcription, replication, or repair. PMID:25934589

  17. POT1 proteins in green algae and land plants: DNA-binding properties and evidence of co-evolution with telomeric DNA

    PubMed Central

    Shakirov, Eugene V.; Song, Xiangyu; Joseph, Jessica A.; Shippen, Dorothy E.

    2009-01-01

    Telomeric DNA terminates with a single-stranded 3′ G-overhang that in vertebrates and fission yeast is bound by POT1 (Protection Of Telomeres). However, no in vitro telomeric DNA binding is associated with Arabidopsis POT1 paralogs. To further investigate POT1–DNA interaction in plants, we cloned POT1 genes from 11 plant species representing major branches of plant kingdom. Telomeric DNA binding was associated with POT1 proteins from the green alga Ostreococcus lucimarinus and two flowering plants, maize and Asparagus. Site-directed mutagenesis revealed that several residues critical for telomeric DNA recognition in vertebrates are functionally conserved in plant POT1 proteins. However, the plant proteins varied in their minimal DNA-binding sites and nucleotide recognition properties. Green alga POT1 exhibited a strong preference for the canonical plant telomere repeat sequence TTTAGGG with no detectable binding to hexanucleotide telomere repeat TTAGGG found in vertebrates and some plants, including Asparagus. In contrast, POT1 proteins from maize and Asparagus bound TTAGGG repeats with only slightly reduced affinity relative to the TTTAGGG sequence. We conclude that the nucleic acid binding site in plant POT1 proteins is evolving rapidly, and that the recent acquisition of TTAGGG telomere repeats in Asparagus appears to have co-evolved with changes in POT1 DNA sequence recognition. PMID:19783822

  18. The effect of Pot1 binding on the repair of thymine analogs in a telomeric DNA sequence.

    PubMed

    Theruvathu, Jacob A; Darwanto, Agus; Hsu, Chia Wei; Sowers, Lawrence C

    2014-08-01

    Telomeric DNA can form duplex regions or single-stranded loops that bind multiple proteins, preventing it from being processed as a DNA repair intermediate. The bases within these regions are susceptible to damage; however, mechanisms for the repair of telomere damage are as yet poorly understood. We have examined the effect of three thymine (T) analogs including uracil (U), 5-fluorouracil (5FU) and 5-hydroxymethyluracil (5hmU) on DNA-protein interactions and DNA repair within the GGTTAC telomeric sequence. The replacement of T with U or 5FU interferes with Pot1 (Pot1pN protein of Schizosaccharomyces pombe) binding. Surprisingly, 5hmU substitution only modestly diminishes Pot1 binding suggesting that hydrophobicity of the T-methyl group likely plays a minor role in protein binding. In the GGTTAC sequence, all three analogs can be cleaved by DNA glycosylases; however, glycosylase activity is blocked if Pot1 binds. An abasic site at the G or T positions is cleaved by the endonuclease APE1 when in a duplex but not when single-stranded. Abasic site formation thermally destabilizes the duplex that could push a damaged DNA segment into a single-stranded loop. The inability to enzymatically cleave abasic sites in single-stranded telomere regions would block completion of the base excision repair cycle potentially causing telomere attrition.

  19. Avoidance and Potential Remedy Solutions of Chimeras in Reconstructing the Phylogeny of Aphids Using the 16S rRNA Gene of Buchnera: A Case in Lachninae (Hemiptera).

    PubMed

    Chen, Rui; Wang, Zhe; Chen, Jing; Qiao, Ge-Xia

    2015-08-25

    It is known that PCR amplification of highly homologous genes from complex DNA mixtures can generate a significant proportion of chimeric sequences. The 16S rRNA gene is not only widely used in estimating the species diversity of endosymbionts in aphids but also used to explore the co-diversification of aphids and their endosymbionts. Thus, chimeric sequences may lead to the discovery of non-existent endosymbiont species and mislead Buchnera-based phylogenetic analysis that lead to false conclusions. In this study, a high probability (6.49%) of chimeric sequence occurrence was found in the amplified 16S rRNA gene sequences of endosymbionts from aphid species in the subfamily Lachninae. These chimeras are hybrid products of multiple parent sequences from the dominant species of endosymbionts in each corresponding host. It is difficult to identify the chimeric sequences of a new or unidentified species due to the high variability of their main parent, Buchnera aphidicola, and because the chimeric sequences can confuse the phylogenetic analysis of 16S rRNA gene sequences. These chimeras present a challenge to Buchnera-based phylogenetic research in aphids. Thus, our study strongly suggests that using appropriate methods to detect chimeric 16S rRNA sequences may avoid some false conclusions in endosymbiont-based aphid research.

  20. FOXP2 promotes the nuclear translocation of POT1, but FOXP2(R553H), mutation related to speech-language disorder, partially prevents it

    SciTech Connect

    Tanabe, Yuko; Fujita, Eriko; Momoi, Takashi

    2011-07-08

    Highlights: {yields} We isolated protection of telomeres 1 (POT1) as a FOXP2-associated protein by a yeast two-hybrid. {yields} FOXP2 associated and co-localized with POT1 in the nuclei. {yields} FOXP2(R553H) also co-localized with POT1 in both the cytoplasm and nuclei. {yields} FOXP2(R553H) partially prevented the nuclear translocation of POT1. {yields} FOXP2(R553H) mutation may be associated with the pathogenesis of speech-language disorder. -- Abstract: FOXP2 is a forkhead box-containing transcription factor with several recognizable sequence motifs. However, little is known about the FOXP2-associated proteins except for C-terminal binding protein (CtBP). In the present study, we attempted to isolate the FOXP2-associated protein with a yeast two-hybrid system using the C-terminal region, including the forkhead domain, as a bait probe, and identified protection of telomeres 1 (POT1) as a FOXP2-associated protein. Immunoprecipitation assay confirmed the association with FOXP2 and POT1. POT1 alone localized in the cytoplasm but co-localized with FOXP2 and the forkhead domain of FOXP2 in nuclei. However, both FOXP2 with mutated nuclear localization signals and (R553H) mutated forkhead, which is associated with speech-language disorder, prevented the nuclear translocation of POT1. These results suggest that FOXP2 is a binding partner for the nuclear translocation of POT1. As loss of POT1 function induces the cell arrest, the impaired nuclear translocation of POT1 in the developing neuronal cells may be associated with the pathogenesis of speech-language disorder with FOXP2(R553H) mutation.

  1. Neuron type-specific miRNA represses two broadly expressed genes to modulate an avoidance behavior in C. elegans

    PubMed Central

    Drexel, Tanja; Mahofsky, Katharina; Latham, Richard; Zimmer, Manuel

    2016-01-01

    Two broad gene classes are distinguished within multicellular organisms: cell type-specific genes, which confer particular cellular properties, and ubiquitous genes that support general cellular functions. However, certain so-called ubiquitous genes show functionally relevant cell type-specific repression. How such repression is achieved is poorly understood. MicroRNAs (miRNAs) are repressors, many of which are expressed with high cell type specificity. Here we show that mir-791, expressed exclusively in the CO2-sensing neurons in Caenorhabditis elegans, represses two otherwise broadly expressed genes. This repression is necessary for normal neuronal function and behavior of the animals toward CO2. miRNA-mediated repression of broadly transcribed genes is a previously unappreciated strategy for cellular specialization. PMID:27688400

  2. FOXP2 promotes the nuclear translocation of POT1, but FOXP2(R553H), mutation related to speech-language disorder, partially prevents it.

    PubMed

    Tanabe, Yuko; Fujita, Eriko; Momoi, Takashi

    2011-07-08

    FOXP2 is a forkhead box-containing transcription factor with several recognizable sequence motifs. However, little is known about the FOXP2-associated proteins except for C-terminal binding protein (CtBP). In the present study, we attempted to isolate the FOXP2-associated protein with a yeast two-hybrid system using the C-terminal region, including the forkhead domain, as a bait probe, and identified protection of telomeres 1 (POT1) as a FOXP2-associated protein. Immunoprecipitation assay confirmed the association with FOXP2 and POT1. POT1 alone localized in the cytoplasm but co-localized with FOXP2 and the forkhead domain of FOXP2 in nuclei. However, both FOXP2 with mutated nuclear localization signals and (R553H) mutated forkhead, which is associated with speech-language disorder, prevented the nuclear translocation of POT1. These results suggest that FOXP2 is a binding partner for the nuclear translocation of POT1. As loss of POT1 function induces the cell arrest, the impaired nuclear translocation of POT1 in the developing neuronal cells may be associated with the pathogenesis of speech-language disorder with FOXP2(R553H) mutation.

  3. RNA.

    ERIC Educational Resources Information Center

    Darnell, James E., Jr.

    1985-01-01

    Ribonucleic acid (RNA) converts genetic information into protein and usually must be processed to serve its function. RNA types, chemical structure, protein synthesis, translation, manufacture, and processing are discussed. Concludes that the first genes might have been spliced RNA and that humans might be closer than bacteria to primitive…

  4. Shade Avoidance

    PubMed Central

    Casal, Jorge J.

    2012-01-01

    The presence of neighboring vegetation modifies the light environment experienced by plants, generating signals that are perceived by phytochromes and cryptochromes. These signals cause large changes in plant body form and function, including enhanced growth of the hypocotyl and petioles, a more erect position of the leaves and early flowering in Arabidopsis thaliana. Collectively, these so-called shade-avoidance responses tend to reduce the degree of current or future shade by neighbors. Shade light signals increase the abundance of PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and PIF5 proteins, promote the synthesis and redirection of auxin, favor the degradation of DELLA proteins and increase the expression of auxin, gibberellins and brassinosteroid-promoted genes, among other events downstream the photoreceptors. Selectively disrupting these events by genetic or pharmacological approaches affects shade-avoidance responses with an intensity that depends on the developmental context and the environment. Shade-avoidance responses provide a model to investigate the signaling networks used by plants to take advantage of the cues provided by the environment to adjust to the challenges imposed by the environment itself. PMID:22582029

  5. A mutation in the POT1 gene is responsible for cardiac angiosarcoma in TP53-negative Li-Fraumeni-like families.

    PubMed

    Calvete, Oriol; Martinez, Paula; Garcia-Pavia, Pablo; Benitez-Buelga, Carlos; Paumard-Hernández, Beatriz; Fernandez, Victoria; Dominguez, Fernando; Salas, Clara; Romero-Laorden, Nuria; Garcia-Donas, Jesus; Carrillo, Jaime; Perona, Rosario; Triviño, Juan Carlos; Andrés, Raquel; Cano, Juana María; Rivera, Bárbara; Alonso-Pulpon, Luis; Setien, Fernando; Esteller, Manel; Rodriguez-Perales, Sandra; Bougeard, Gaelle; Frebourg, Tierry; Urioste, Miguel; Blasco, Maria A; Benítez, Javier

    2015-09-25

    Cardiac angiosarcoma (CAS) is a rare malignant tumour whose genetic basis is unknown. Here we show, by whole-exome sequencing of a TP53-negative Li-Fraumeni-like (LFL) family including CAS cases, that a missense variant (p.R117C) in POT1 (protection of telomeres 1) gene is responsible for CAS. The same gene alteration is found in two other LFL families with CAS, supporting the causal effect of the identified mutation. We extend the analysis to TP53-negative LFL families with no CAS and find the same mutation in a breast AS family. The mutation is recently found once in 121,324 studied alleles in ExAC server but it is not described in any other database or found in 1,520 Spanish controls. In silico structural analysis suggests how the mutation disrupts POT1 structure. Functional and in vitro studies demonstrate that carriers of the mutation show reduced telomere-bound POT1 levels, abnormally long telomeres and increased telomere fragility.

  6. A mutation in the POT1 gene is responsible for cardiac angiosarcoma in TP53-negative Li–Fraumeni-like families

    PubMed Central

    Calvete, Oriol; Martinez, Paula; Garcia-Pavia, Pablo; Benitez-Buelga, Carlos; Paumard-Hernández, Beatriz; Fernandez, Victoria; Dominguez, Fernando; Salas, Clara; Romero-Laorden, Nuria; Garcia-Donas, Jesus; Carrillo, Jaime; Perona, Rosario; Triviño, Juan Carlos; Andrés, Raquel; Cano, Juana María; Rivera, Bárbara; Alonso-Pulpon, Luis; Setien, Fernando; Esteller, Manel; Rodriguez-Perales, Sandra; Bougeard, Gaelle; Frebourg, Tierry; Urioste, Miguel; Blasco, Maria A.; Benítez, Javier

    2015-01-01

    Cardiac angiosarcoma (CAS) is a rare malignant tumour whose genetic basis is unknown. Here we show, by whole-exome sequencing of a TP53-negative Li–Fraumeni-like (LFL) family including CAS cases, that a missense variant (p.R117C) in POT1 (protection of telomeres 1) gene is responsible for CAS. The same gene alteration is found in two other LFL families with CAS, supporting the causal effect of the identified mutation. We extend the analysis to TP53-negative LFL families with no CAS and find the same mutation in a breast AS family. The mutation is recently found once in 121,324 studied alleles in ExAC server but it is not described in any other database or found in 1,520 Spanish controls. In silico structural analysis suggests how the mutation disrupts POT1 structure. Functional and in vitro studies demonstrate that carriers of the mutation show reduced telomere-bound POT1 levels, abnormally long telomeres and increased telomere fragility. PMID:26403419

  7. Teachers Avoiding Learners' Avoidance: Is It Possible?

    ERIC Educational Resources Information Center

    Tadayyon, Maedeh; Zarrinabadi, Nourollah; Ketabi, Saeed

    2016-01-01

    Dealing with learners who prefer to take the back seat and avoid classroom participation can be every teacher's nightmare. This lack of participation may cause teacher frustration, and possibly the only way to reduce this lack of participation is to access the concept of avoidance strategy. Avoidance strategy is the abandonment of a classroom task…

  8. Avoiding the Flu

    MedlinePlus

    ... of this page please turn Javascript on. Feature: Flu Avoiding the Flu Past Issues / Fall 2009 Table of Contents Children ... help avoid getting and passing on the flu. Influenza (Seasonal) The flu is a contagious respiratory illness ...

  9. Avoiding health information.

    PubMed

    Barbour, Joshua B; Rintamaki, Lance S; Ramsey, Jason A; Brashers, Dale E

    2012-01-01

    This study investigated why and how individuals avoid health information to support the development of models of uncertainty and information management and offer insights for those dealing with the information and uncertainty inherent to health and illness. Participants from student (n = 507) and community (n = 418) samples reported that they avoided health information to (a) maintain hope or deniability, (b) resist overexposure, (c) accept limits of action, (d) manage flawed information, (e) maintain boundaries, and (f) continue with life/activities. They also reported strategies for avoiding information, including removing or ignoring stimuli (e.g., avoiding people who might provide health advice) and controlling conversations (e.g., withholding information, changing the subject). Results suggest a link between previous experience with serious illness and health information avoidance. Building on uncertainty management theory, this study demonstrated that health information avoidance is situational, relatively common, not necessarily unhealthy, and may be used to accomplish multiple communication goals.

  10. Avoiding Cancer Risk Information

    PubMed Central

    Emanuel, Amber S.; Kiviniemi, Marc T.; Howell, Jennifer L.; Hay, Jennifer L.; Waters, Erika A.; Orom, Heather; Shepperd, James A.

    2015-01-01

    RATIONALE Perceived risk for health problems such as cancer is a central construct in many models of health decision making and a target for behavior change interventions. However, some portion of the population actively avoids cancer risk information. The prevalence of, explanations for, and consequences of such avoidance are not well understood. OBJECTIVE We examined the prevalence and demographic and psychosocial correlates of cancer risk information avoidance preference in a nationally representative sample. We also examined whether avoidance of cancer risk information corresponds with avoidance of cancer screening. RESULTS Based on our representative sample, 39% of the population indicated that they agreed or strongly agreed that they would “rather not know [their] chance of getting cancer.” This preference was stronger among older participants, female participants, and participants with lower levels of education. Preferring to avoid cancer risk information was stronger among participants who agreed with the beliefs that everything causes cancer, that there’s not much one can do to prevent cancer, and that there are too many recommendations to follow. Finally, the preference to avoid cancer risk information was associated with lower levels of screening for colon cancer. CONCLUSION These findings suggest that cancer risk information avoidance is a multi-determined phenomenon that is associated with demographic characteristics and psychosocial individual differences and also relates to engagement in cancer screening. PMID:26560410

  11. Avoiding Computer Viruses.

    ERIC Educational Resources Information Center

    Rowe, Joyce; And Others

    1989-01-01

    The threat of computer sabotage is a real concern to business teachers and others responsible for academic computer facilities. Teachers can minimize the possibility. Eight suggestions for avoiding computer viruses are given. (JOW)

  12. Avoidant personality disorder

    MedlinePlus

    American Psychiatric Association. Avoidant personality disorder. Diagnostic and Statistical Manual of Mental Disorders: DSM-5 . 5th ed. Arlington, VA: American Psychiatric Publishing. 2013;672-675. Blais MA, Smallwood ...

  13. Operational Collision Avoidance

    NASA Technical Reports Server (NTRS)

    Guit, Bill

    2015-01-01

    This presentation will describe the early days of the EOS Aqua and Aura operational collision avoidance process. It will highlight EOS debris avoidance maneuvers, EOS high interest event statistic and A-Train systematic conjunctions and conclude with future challenges. This is related to earlier e-DAA (tracking number 21692) that an abstract was submitted to a different conference. Eric Moyer, ESMO Deputy Project Manager has reviewed and approved this presentation on May 6, 2015

  14. Reactive Collision Avoidance Algorithm

    NASA Technical Reports Server (NTRS)

    Scharf, Daniel; Acikmese, Behcet; Ploen, Scott; Hadaegh, Fred

    2010-01-01

    The reactive collision avoidance (RCA) algorithm allows a spacecraft to find a fuel-optimal trajectory for avoiding an arbitrary number of colliding spacecraft in real time while accounting for acceleration limits. In addition to spacecraft, the technology can be used for vehicles that can accelerate in any direction, such as helicopters and submersibles. In contrast to existing, passive algorithms that simultaneously design trajectories for a cluster of vehicles working to achieve a common goal, RCA is implemented onboard spacecraft only when an imminent collision is detected, and then plans a collision avoidance maneuver for only that host vehicle, thus preventing a collision in an off-nominal situation for which passive algorithms cannot. An example scenario for such a situation might be when a spacecraft in the cluster is approaching another one, but enters safe mode and begins to drift. Functionally, the RCA detects colliding spacecraft, plans an evasion trajectory by solving the Evasion Trajectory Problem (ETP), and then recovers after the collision is avoided. A direct optimization approach was used to develop the algorithm so it can run in real time. In this innovation, a parameterized class of avoidance trajectories is specified, and then the optimal trajectory is found by searching over the parameters. The class of trajectories is selected as bang-off-bang as motivated by optimal control theory. That is, an avoiding spacecraft first applies full acceleration in a constant direction, then coasts, and finally applies full acceleration to stop. The parameter optimization problem can be solved offline and stored as a look-up table of values. Using a look-up table allows the algorithm to run in real time. Given a colliding spacecraft, the properties of the collision geometry serve as indices of the look-up table that gives the optimal trajectory. For multiple colliding spacecraft, the set of trajectories that avoid all spacecraft is rapidly searched on

  15. Vigour in active avoidance.

    PubMed

    Nord, Camilla L; Prabhu, Gita; Nolte, Tobias; Fonagy, Peter; Dolan, Ray; Moutoussis, Michael

    2017-12-01

    It would be maladaptive to learn about catastrophes by trial and error alone. Investment in planning and effort are necessary. Devoting too many resources to averting disaster, however, can impair quality of life, as in anxiety and paranoia. Here, we developed a novel task to explore how people adjust effort expenditure (vigor) so as to avoid negative consequences. Our novel paradigm is immersive, enabling us to measure vigor in the context of (simulated) disaster. We found that participants (N = 118) exerted effort to avoid disaster-associated states, adjusting their effort expenditure according to the baseline probability of catastrophe, in agreement with theoretical predictions. Furthermore, negative subjective emotional states were associated both with threat level and with increasing vigor in the face of disaster. We describe for the first time effort expenditure in the context of irreversible losses, with important implications for disorders marked by excessive avoidance.

  16. Psychological Treatments to Avoid

    ERIC Educational Resources Information Center

    Thomason, Timothy C.

    2010-01-01

    Certain psychological treatments should be avoided, and a list of such treatments would provide valuable guidance for counselors, as well as potential clients. It is well established that some therapies are potentially dangerous, and some fringe therapies are highly unlikely to help clients beyond a placebo effect. This article provides an…

  17. Plants to Avoid

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Knowledge of poisonous plants is extremely important for home owners, gardeners, farmers, hunters, hikers, and the rest of the general public. Among the most important plants to avoid in the Delta Region are poison ivy, bull nettle, eastern black nightshade, Queen Ann’s lace, jimsonweed, and trumpe...

  18. Avoiding Death by Vacuum

    NASA Astrophysics Data System (ADS)

    Barroso, A.; Ferreira, P. M.; Ivanov, I.; Santos, R.; Silva, João P.

    2013-07-01

    The two-Higgs doublet model (2HDM) can have two electroweak breaking, CP-conserving, minima. The possibility arises that the minimum which corresponds to the known elementary particle spectrum is metastable, a possibility we call the "panic vacuum". We present analytical bounds on the parameters of the softly broken Peccei-Quinn 2HDM which are necessary and sufficient conditions to avoid this possibility. We also show that, for this particular model, the current LHC data already tell us that we are necessarily in the global minimum of the theory, regardless of any cosmological considerations about the lifetime of the false vacua.

  19. Landing Hazard Avoidance Display

    NASA Technical Reports Server (NTRS)

    Abernathy, Michael Franklin (Inventor); Hirsh, Robert L. (Inventor)

    2016-01-01

    Landing hazard avoidance displays can provide rapidly understood visual indications of where it is safe to land a vehicle and where it is unsafe to land a vehicle. Color coded maps can indicate zones in two dimensions relative to the vehicles position where it is safe to land. The map can be simply green (safe) and red (unsafe) areas with an indication of scale or can be a color coding of another map such as a surface map. The color coding can be determined in real time based on topological measurements and safety criteria to thereby adapt to dynamic, unknown, or partially known environments.

  20. Predicting attention and avoidance: when do avoiders attend?

    PubMed

    Klein, Rupert; Knäuper, Bärbel

    2009-09-01

    Three avoidance measures, the Miller Behavioural Style Scale (MBSS), Index of Self-Regulation of Emotion (ISE) and Mainz Coping Inventory (MCI), were compared in their ability to predict attention and avoidance of threats in the emotional Stroop task. It was also examined if the avoidance mechanism of individuals who would normally avoid threat-indicating words becomes disrupted under conditions of dopamine reduction. Results show that only the ISE predicted attention/avoidance of threat-indicating words. In addition, the avoidance mechanism, as measured by the ISE and MCI, was not activated when regular smokers abstained from smoking.

  1. Avoiding dangerous climate change

    SciTech Connect

    Hans Joachim Schellnhuber; Wolfgang Cramer; Nebojsa Nakicenovic; Tom Wigley; Gary Yohe

    2006-02-15

    In 2005 the UK Government hosted the Avoiding Dangerous Climate Change conference to take an in-depth look at the scientific issues associated with climate change. This volume presents the most recent findings from the leading international scientists that attended the conference. The topics addressed include critical thresholds and key vulnerabilities of the climate system, impacts on human and natural systems, socioeconomic costs and benefits of emissions pathways, and technological options for meeting different stabilisation levels of greenhouse gases in the atmosphere. Contents are: Foreword from Prime Minister Tony Blair; Introduction from Rajendra Pachauri, Chairman of the IPCC; followed by 41 papers arranged in seven sections entitled: Key Vulnerabilities of the Climate System and Critical Thresholds; General Perspectives on Dangerous Impacts; Key Vulnerabilities for Ecosystems and Biodiversity; Socio-Economic Effects; Regional Perspectives; Emission Pathways; and Technological Options. Four papers have been abstracted separately for the Coal Abstracts database.

  2. Measuring Experiential Avoidance in Adults: The Avoidance and Fusion Questionnaire

    ERIC Educational Resources Information Center

    Schmalz, Jonathan E.; Murrell, Amy R.

    2010-01-01

    To date, general levels of experiential avoidance are primarily measured by the Acceptance and Action Questionnaire-II (AAQ-II), but it includes items of questionable comprehensibility. The Avoidance and Fusion Questionnaire for Youth (AFQ-Y), previously validated as a measure of experiential avoidance with children and adolescents, was…

  3. Avoiding Infection After Ear Piercing

    MedlinePlus

    ... Text Size Email Print Share Avoiding Infection After Ear Piercing Page Content Article Body What is the best way to avoid infection after ear piercing? Ears may be pierced for cosmetic reasons ...

  4. CAT altitude avoidance system

    NASA Technical Reports Server (NTRS)

    Gary, B. L. (Inventor)

    1982-01-01

    A method and apparatus are provided for indicating the altitude of the tropopause or of an inversion layer wherein clear air turbulence (CAT) may occur, and the likely severity of any such CAT, includes directing a passive microwave radiometer on the aircraft at different angles with respect to the horizon. The microwave radiation measured at a frequency of about 55 GHz represents the temperature of the air at an ""average'' range of about 3 kilometers, so that the sine of the angle of the radiometer times 3 kilometers equals the approximate altitude of the air whose temperature is measured. A plot of altitude (with respect to the aircraft) versus temperature of the air at that altitude, can indicate when an inversion layer is present and can indicate the altitude of the tropopause or of such an inversion layer. The plot can also indicate the severity of any CAT in an inversion layer. If CAT has been detected in the general area, then the aircraft can be flown at an altitude to avoid the tropopause or inversion layer.

  5. Collision avoidance sensor skin

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The objective was to totally eliminate the possibility of a robot (or any mechanism for that matter) inducing a collision in space operations. We were particularly concerned that human beings were safe under all circumstances. This was apparently accomplished, and it is shown that GSFC has a system that is ready for space qualification and flight. However, it soon became apparent that much more could be accomplished with this technology. Payloads could be made invulnerable to collision avoidance and the blind spots behind them eliminated. This could be accomplished by a simple, non-imaging set of 'Capaciflector' sensors on each payload. It also is evident that this system could be used to align and dock the system with a wide margin of safety. Throughout, lighting problems could be ignored, and unexpected events and modeling errors taken in stride. At the same time, computational requirements would be reduced. This can be done in a simple, rugged, reliable manner that will not disturb the form factor of space systems. It will be practical for space applications. The lab experiments indicate we are well on the way to accomplishing this. Still, the research trail goes deeper. It now appears that the sensors can be extended to end effectors to provide precontact information and make robot docking (or any docking connection) very smooth, with minimal loads impacted back into the mating structures. This type of ability would be a major step forward in basic control techniques in space. There are, however, baseline and restructuring issues to be tackled. The payloads must get power and signals to them from the robot or from the astronaut servicing tool. This requires a standard electromechanical interface. Any of several could be used. The GSFC prototype shown in this presentation is a good one. Sensors with their attendant electronics must be added to the payloads, end effectors, and robot arms and integrated into the system.

  6. MEST- avoid next extinction

    NASA Astrophysics Data System (ADS)

    Cao, Dayong

    2012-11-01

    Asteroid 2011 AG5 will impact on Earth in 2040. (See Donald K. Yoemans, ``Asteroid 2011 AG5 - A Reality Check,'' NASA-JPL, 2012) In 2011, The author say: the dark hole will take the dark comet to impact our solar system in 20 years, and give a systemic model between the sun and its companion-dark hole to explain why were there periodicity mass extinction on earth. (see Dayong Cao, BAPS.2011.CAL.C1.7, BAPS.2011.DFD.LA.24, BAPS.2012.APR.K1.78 and BAPS.2011.APR.K1.17) The dark Asteroid 2011 AG5 (as a dark comet) is made of the dark matter which has a space-time (as frequence-amplitude square) center- a different systemic model from solar systemic model. It can asborb the space-time and wave. So it is ``dark.'' When many dark matters hit on our earth, they can break our atom structure and our genetic code to trigger the Mass Extinction. In our experiments, consciousness can change the systematic model and code by a life-informational technology. So it can change the output signals of the solar cell. (see Dayong Cao, BAPS.2011.MAR.C1.286 and BAPS.2012.MAR.P33.14) So we will develop the genetic code of lives to evolution and sublimation, will use the dark matter to change the systemic model between dark hole and sun and will avoid next extinction.

  7. Stimulus conflict triggers behavioral avoidance.

    PubMed

    Dignath, David; Eder, Andreas B

    2015-12-01

    According to a recent extension of the conflict-monitoring theory, conflict between two competing response tendencies is registered as an aversive event and triggers a motivation to avoid the source of conflict. In the present study, we tested this assumption. Over five experiments, we examined whether conflict is associated with an avoidance motivation and whether stimulus conflict or response conflict triggers an avoidance tendency. Participants first performed a color Stroop task. In a subsequent motivation test, participants responded to Stroop stimuli with approach- and avoidance-related lever movements. These results showed that Stroop-conflict stimuli increased the frequency of avoidance responses in a free-choice motivation test, and also increased the speed of avoidance relative to approach responses in a forced-choice test. High and low proportions of response conflict in the Stroop task had no effect on avoidance in the motivation test. Avoidance of conflict was, however, obtained even with new conflict stimuli that had not been presented before in a Stroop task, and when the Stroop task was replaced with an unrelated filler task. Taken together, these results suggest that stimulus conflict is sufficient to trigger avoidance.

  8. Chemical avoidance responses of fishes.

    PubMed

    Tierney, Keith B

    2016-05-01

    The hydrosphere is a repository for all of our waste and mistakes, be they sewage, garbage, process-affected waters, runoff, and gases. For fish living in environments receiving undesirable inputs, moving away seems an obvious way to avoid harm. While this should occur, there are numerous examples where it will not. The inability to avoid harmful environments may lead to sensory impairments that in turn limit the ability to avoid other dangers or locate benefits. For avoidance to occur, the danger must first be perceived, which may not happen if the fish is 'blinded' in some capacity. Second, the danger must be recognized for what it is, which may also not happen if the fish is cognitively confused or impaired. Third, it is possible that the fish may not be able to leave the area, or worse, learns to prefer a toxic environment. Concerning generating regulations around avoidance, there are two possibilities: that an avoidance threshold be used to set guidelines for effluent release with the intention of driving fishes away; the second is to set a contaminant concentration that would not affect the avoidance or attraction responses to other cues. With the complexities of the modern world in which we release diverse pollutants, from light to municipal effluents full of 1000s of chemicals, to the diversity present in ecosystems, it is impossible to have avoidance data on every stimulus-species combination. Nevertheless, we may be able to use existing avoidance response data to predict the likelihood of avoidance of untested stimuli. Where we cannot, this review includes a framework that can be used to direct new research. This review is intended to collate existing avoidance response data, provide a framework for making decisions in the absence of data, and suggest studies that would facilitate the prediction of risk to fish health in environments receiving intentional and unintentional human-based chemical inputs.

  9. RNA Interference

    MedlinePlus

    ... NIGMS Home > Science Education > RNA Interference Fact Sheet RNA Interference Fact Sheet Tagline (Optional) Middle/Main Content Area What is RNA interference? RNA interference (RNAi) is a natural process ...

  10. Postcopulatory inbreeding avoidance in guppies.

    PubMed

    Fitzpatrick, J L; Evans, J P

    2014-12-01

    In many species, the negative fitness effects of inbreeding have facilitated the evolution of a wide range of inbreeding avoidance mechanisms. Although avoidance mechanisms operating prior to mating are well documented, evidence for postcopulatory mechanisms of inbreeding avoidance remain scarce. Here, we examine the potential for paternity biases to favour unrelated males when their sperm compete for fertilizations though postcopulatory inbreeding avoidance mechanisms in the guppy, Poecilia reticulata. To test this possibility, we used a series of artificial inseminations to deliver an equal number of sperm from a related (either full sibling or half sibling) and unrelated male to a female while statistically controlling for differences in sperm quality between rival ejaculates. In this way, we were able to focus exclusively on postcopulatory mechanisms of inbreeding avoidance and account for differences in sperm competitiveness between rival males. Under these carefully controlled conditions, we report a significant bias in paternity towards unrelated males, although this effect was only apparent when the related male was a full sibling. We also show that sperm competition generally favours males with highly viable sperm and thus that some variance in sperm competitiveness can be attributed to difference in sperm quality. Our findings for postcopulatory inbreeding avoidance are consistent with prior work on guppies, revealing that sperm competition success declines linearly with the level of relatedness, but also that such effects are only apparent at relatedness levels of full siblings or higher. These findings reveal that postcopulatory processes alone can facilitate inbreeding avoidance.

  11. Vertical jumping and signaled avoidance

    PubMed Central

    Cándido, Antonio; Maldonado, Antonio; Vila, Jaime

    1988-01-01

    This paper reports an experiment intended to demonstrate that the vertical jumping response can be learned using a signaled-avoidance technique. A photoelectric cell system was used to record the response. Twenty female rats, divided equally into two groups, were exposed to intertrial intervals of either 15 or 40 s. Subjects had to achieve three successive criteria of acquisition: 3, 5, and 10 consecutive avoidance responses. Results showed that both groups learned the avoidance response, requiring increasingly larger numbers of trials as the acquisition criteria increased. No significant effect of intertrial interval was observed. PMID:16812559

  12. How to avoid exercise injuries

    MedlinePlus

    ... gov/ency/patientinstructions/000859.htm How to avoid exercise injuries To use the sharing features on this ... injury and stay safe during exercise. What Causes Exercise Injuries? Some of the most common causes of ...

  13. Vision-based obstacle avoidance

    DOEpatents

    Galbraith, John

    2006-07-18

    A method for allowing a robot to avoid objects along a programmed path: first, a field of view for an electronic imager of the robot is established along a path where the electronic imager obtains the object location information within the field of view; second, a population coded control signal is then derived from the object location information and is transmitted to the robot; finally, the robot then responds to the control signal and avoids the detected object.

  14. Airborne Collision Avoidance System X

    DTIC Science & Technology

    2015-06-01

    avoidance system on behalf of the Federal Aviation Adminis- tration (FAA). The current Traffic Alert and Collision Avoidance System II (TCAS II...which are used on board an aircraft. The tables provide a cost for each action—no alert , a traffic advisory alerting pilots about nearby aircraft, or a...suitabil- ity than does TCAS II; studies show that ACAS X reduces mid-air collision risk by 59% and unnecessary disruptive alerts by 25% when

  15. Predator Avoidance in Extremophile Fish

    PubMed Central

    Bierbach, David; Schulte, Matthias; Herrmann, Nina; Zimmer, Claudia; Arias-Rodriguez, Lenin; Indy, Jeane Rimber; Riesch, Rüdiger; Plath, Martin

    2013-01-01

    Extreme habitats are often characterized by reduced predation pressures, thus representing refuges for the inhabiting species. The present study was designed to investigate predator avoidance of extremophile populations of Poecilia mexicana and P. sulphuraria that either live in hydrogen sulfide-rich (sulfidic) springs or cave habitats, both of which are known to have impoverished piscine predator regimes. Focal fishes that inhabited sulfidic springs showed slightly weaker avoidance reactions when presented with several naturally occurring predatory cichlids, but strongest differences to populations from non-sulfidic habitats were found in a decreased shoaling tendency with non-predatory swordtail (Xiphophorus hellerii) females. When comparing avoidance reactions between P. mexicana from a sulfidic cave (Cueva del Azufre) and the adjacent sulfidic surface creek (El Azufre), we found only slight differences in predator avoidance, but surface fish reacted much more strongly to the non-predatory cichlid Vieja bifasciata. Our third experiment was designed to disentangle learned from innate effects of predator recognition. We compared laboratory-reared (i.e., predator-naïve) and wild-caught (i.e., predator-experienced) individuals of P. mexicana from a non-sulfidic river and found no differences in their reaction towards the presented predators. Overall, our results indicate (1) that predator avoidance is still functional in extremophile Poecilia spp. and (2) that predator recognition and avoidance reactions have a strong genetic basis. PMID:25371337

  16. Juan Bobo and the Pot 1.

    ERIC Educational Resources Information Center

    Flores, Nydia; And Others

    This story and coloring book is one in a series of Puerto Rican folktale books and is designed for use in a bilingual/bicultural education setting. This volume is geared to those students just beginning to learn English. The illustrated story is followed by a teacher's guide, consisting of information on the series, background on Puerto Rican…

  17. Automatic Collision Avoidance Technology (ACAT)

    NASA Technical Reports Server (NTRS)

    Swihart, Donald E.; Skoog, Mark A.

    2007-01-01

    This document represents two views of the Automatic Collision Avoidance Technology (ACAT). One viewgraph presentation reviews the development and system design of Automatic Collision Avoidance Technology (ACAT). Two types of ACAT exist: Automatic Ground Collision Avoidance (AGCAS) and Automatic Air Collision Avoidance (AACAS). The AGCAS Uses Digital Terrain Elevation Data (DTED) for mapping functions, and uses Navigation data to place aircraft on map. It then scans DTED in front of and around aircraft and uses future aircraft trajectory (5g) to provide automatic flyup maneuver when required. The AACAS uses data link to determine position and closing rate. It contains several canned maneuvers to avoid collision. Automatic maneuvers can occur at last instant and both aircraft maneuver when using data link. The system can use sensor in place of data link. The second viewgraph presentation reviews the development of a flight test and an evaluation of the test. A review of the operation and comparison of the AGCAS and a pilot's performance are given. The same review is given for the AACAS is given.

  18. Approach/avoidance in dreams.

    PubMed

    Malcolm-Smith, Susan; Koopowitz, Sheri; Pantelis, Eleni; Solms, Mark

    2012-03-01

    The influential threat simulation theory (TST) asserts that dreaming yields adaptive advantage by providing a virtual environment in which threat-avoidance may be safely rehearsed. We have previously found the incidence of biologically threatening dreams to be around 20%, with successful threat avoidance occurring in approximately one-fifth of such dreams. TST asserts that threat avoidance is over-represented relative to other possible dream contents. To begin assessing this issue, we contrasted the incidence of 'avoidance' dreams with that of their opposite: 'approach' dreams. Because TST states that the threat-avoidance function is only fully activated in ecologically valid (biologically threatening) contexts, we also performed this contrast for populations living in both high- and low-threat environments. We find that 'approach' dreams are significantly more prevalent across both contexts. We suggest these results are more consistent with the view that dreaming is generated by reward-seeking systems than by fear-conditioning systems, although reward-seeking is clearly not the only factor determining the content of dreams.

  19. Motive to Avoid Success, Locus of Control, and Reinforcement Avoidance.

    ERIC Educational Resources Information Center

    Katovsky, Walter

    Subjects were four groups of 12 college women, high or low in motive to avoid success (MAS) and locus of control (LC), were reinforced for response A on a fixed partial reinforcement schedule on three concept learning tasks, one task consisting of combined reward and punishment, another of reward only, and one of punishment only. Response B was…

  20. Food Avoidance Diets for Dermatitis.

    PubMed

    Scott, Jeffrey F; Hammond, Margaret I; Nedorost, Susan T

    2015-10-01

    Food allergy is relatively common in both children and adults, and its prevalence is increasing. Early exposure of food allergens onto skin with an impaired epidermal barrier predisposes to sensitization and prevents the development of oral tolerance. While immediate-type food allergies are well described, less is known about delayed-type food allergies manifesting as dermatitis. This is due, in part, to limitations with current diagnostic testing for delayed-type food allergy, including atopy patch testing. We conducted a systematic review of food avoidance diets in delayed-type food allergies manifesting as dermatitis. While beneficial in some clinical circumstances, avoidance diets should be used with caution in infants and children, as growth impairment and developmental delay may result. Ultimately, dermatitis is highly multifactorial and avoidance diets may not improve symptoms of delayed-type food allergy until combined with other targeted therapies, including restoring balance in the skin microbiome and re-establishing proper skin barrier function.

  1. A problem of collision avoidance

    NASA Technical Reports Server (NTRS)

    Vincent, T. L.; Cliff, E. M.; Grantham, W. J.; Peng, W. Y.

    1972-01-01

    Collision avoidance between two vehicles of constant speed with limited turning radii, moving in a horizontal plane is investigated. Collision avoidance is viewed as a game by assuming that the operator of one vehicle has perfect knowledge of the state of the other, whereas the operator of the second vehicle is unaware of any impending danger. The situation envisioned is that of an encounter between a commercial aircraft and a small light aircraft. This worse case situation is examined to determine the conditions under which the commercial aircraft should execute a collision avoidance maneuver. Three different zones of vulnerability are defined and the boundaries, or barriers, between these zones are determined for a typical aircraft encounter. A discussion of the methods used to obtain the results as well as some of the salient features associated with the resultant barriers is included.

  2. Avoiding unfavourable outcomes in liposuction

    PubMed Central

    Khanna, Atul; Filobbos, George

    2013-01-01

    The origin of liposuction can be traced to an adverse event by Dujarrier in 1921 when he used a uterine curette to remove fat from the knees of a ballerina ending in an amputation secondary to damage of the femoral artery. The history of liposuction since then has been one of avoiding complications and optimising outcome. After this adverse event, liposuction was abandoned until the 1960's when Schrudde revived the practice using small stab incisions and sharp curettage with the secondary suction to aspirate the freed tissue. This technique was associated with a high incidence of complications especially seroma and skin necrosis. Illouz then replaced the curette with a blunt cannula connected to vacuum pump thus avoiding the complications of a sharp curette. Despite the presence of various techniques for liposuction, suction assisted liposuction (SAL) is still the standard technique of liposuction. This article aims to discuss literature regarding the various aspects of liposuction (SAL) and to highlight the salient points in the literature and in the senior author's experience in order to avoid unfavourable outcomes in liposuction. A literature review on avoiding complication is in liposuction including some of the seminal papers on liposuction. Liposuction is generally a safe procedure with reproducible outcome. Just like any surgical procedure it should be treated with the utmost care. Illouz published 10 commandments for liposuction in 1989 and we review these commandments to demonstrate how liposuction has evolved. PMID:24501475

  3. Strategies of locomotor collision avoidance.

    PubMed

    Basili, Patrizia; Sağlam, Murat; Kruse, Thibault; Huber, Markus; Kirsch, Alexandra; Glasauer, Stefan

    2013-03-01

    Collision avoidance during locomotion can be achieved by a variety of strategies. While in some situations only a single trajectory will successfully avoid impact, in many cases several different strategies are possible. Locomotor experiments in the presence of static boundary conditions have suggested that the choice of an appropriate trajectory is based on a maximum-smoothness strategy. Here we analyzed locomotor trajectories of subjects avoiding collision with another human crossing their path orthogonally. In such a case, changing walking direction while keeping speed or keeping walking direction while changing speed would be two extremes of solving the problem. Our participants clearly favored changing their walking speed while keeping the path on a straight line between start and goal. To interpret this result, we calculated the costs of the chosen trajectories in terms of a smoothness-maximization criterion and simulated the trajectories with a computational model. Data analysis together with model simulation showed that the experimentally chosen trajectory to avoid collision with a moving human is not the optimally smooth solution. However, even though the trajectory is not globally smooth, it was still locally smooth. Modeling further confirmed that, in presence of the moving human, there is always a trajectory that would be smoother but would deviate from the straight line. We therefore conclude that the maximum smoothness strategy previously suggested for static environments no longer holds for locomotor path planning and execution in dynamically changing environments such as the one tested here.

  4. Biochar aging reduces earthworm avoidance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biochar, a black carbon substance produced by the pyrolysis of organic feedstocks, has been used in many soil improvement strategies ranging from nutrient addition to sequestration of C. Simple toxicity studies and laboratory preference/avoidance assays are recommended but results rarely reported. ...

  5. Omnipotent RNA.

    PubMed

    Spirin, Alexander S

    2002-10-23

    The capability of polyribonucleotide chains to form unique, compactly folded structures is considered the basis for diverse non-genetic functions of RNA, including the function of recognition of various ligands and the catalytic function. Together with well-known genetic functions of RNA - coding and complementary replication - this has led to the concept of the functional omnipotence of RNA and the hypothesis that an ancient RNA world supposedly preceded the contemporary DNA-RNA-protein life. It is proposed that the Woese universal precursor in the ancient RNA world could be a cell-free community of mixed RNA colonies growing and multiplying on solid surfaces.

  6. RNA Crystallization

    NASA Technical Reports Server (NTRS)

    Golden, Barbara L.; Kundrot, Craig E.

    2003-01-01

    RNA molecules may be crystallized using variations of the methods developed for protein crystallography. As the technology has become available to syntheisize and purify RNA molecules in the quantities and with the quality that is required for crystallography, the field of RNA structure has exploded. The first consideration when crystallizing an RNA is the sequence, which may be varied in a rational way to enhance crystallizability or prevent formation of alternate structures. Once a sequence has been designed, the RNA may be synthesized chemically by solid-state synthesis, or it may be produced enzymatically using RNA polymerase and an appropriate DNA template. Purification of milligram quantities of RNA can be accomplished by HPLC or gel electrophoresis. As with proteins, crystallization of RNA is usually accomplished by vapor diffusion techniques. There are several considerations that are either unique to RNA crystallization or more important for RNA crystallization. Techniques for design, synthesis, purification, and crystallization of RNAs will be reviewed here.

  7. RNA helicases

    PubMed Central

    Owttrim, George W.

    2013-01-01

    Similar to proteins, RNA molecules must fold into the correct conformation and associate with protein complexes in order to be functional within a cell. RNA helicases rearrange RNA secondary structure and RNA-protein interactions in an ATP-dependent reaction, performing crucial functions in all aspects of RNA metabolism. In prokaryotes, RNA helicase activity is associated with roles in housekeeping functions including RNA turnover, ribosome biogenesis, translation and small RNA metabolism. In addition, RNA helicase expression and/or activity are frequently altered during cellular response to abiotic stress, implying they perform defined roles during cellular adaptation to changes in the growth environment. Specifically, RNA helicases contribute to the formation of cold-adapted ribosomes and RNA degradosomes, implying a role in alleviation of RNA secondary structure stabilization at low temperature. A common emerging theme involves RNA helicases acting as scaffolds for protein-protein interaction and functioning as molecular clamps, holding RNA-protein complexes in specific conformations. This review highlights recent advances in DEAD-box RNA helicase association with cellular response to abiotic stress in prokaryotes. PMID:23093803

  8. Rapid jamming avoidance in biosonar.

    PubMed

    Gillam, Erin H; Ulanovsky, Nachum; McCracken, Gary F

    2007-03-07

    The sonar systems of bats and dolphins are in many ways superior to man-made sonar and radar systems, and considerable effort has been devoted to understanding the signal-processing strategies underlying these capabilities. A major feature determining the efficiency of sonar systems is the sensitivity to noise and jamming signals. Previous studies indicated that echolocating bats may adjust their signal structure to avoid jamming ('jamming avoidance response'; JAR). However, these studies relied on behavioural correlations and not controlled experiments. Here, we provide the first experimental evidence for JAR in bats. We presented bats (Tadarida brasiliensis) with 'playback stimuli' consisting of recorded echolocation calls at one of six frequencies. The bats exhibited a JAR by shifting their call frequency away from the presented playback frequency. When the approaching bats were challenged by an abrupt change in the playback stimulus, they responded by shifting their call frequencies upwards, away from the playback. Interestingly, even bats initially calling below the playback's frequency shifted their frequencies upwards, 'jumping' over the playback frequency. These spectral shifts in the bats' calls occurred often within less than 200 ms, in the first echolocation call emitted after the stimulus switch-suggesting that rapid jamming avoidance is important for the bat.

  9. Rapid jamming avoidance in biosonar

    PubMed Central

    Gillam, Erin H; Ulanovsky, Nachum; McCracken, Gary F

    2006-01-01

    The sonar systems of bats and dolphins are in many ways superior to man-made sonar and radar systems, and considerable effort has been devoted to understanding the signal-processing strategies underlying these capabilities. A major feature determining the efficiency of sonar systems is the sensitivity to noise and jamming signals. Previous studies indicated that echolocating bats may adjust their signal structure to avoid jamming (‘jamming avoidance response’; JAR). However, these studies relied on behavioural correlations and not controlled experiments. Here, we provide the first experimental evidence for JAR in bats. We presented bats (Tadarida brasiliensis) with ‘playback stimuli’ consisting of recorded echolocation calls at one of six frequencies. The bats exhibited a JAR by shifting their call frequency away from the presented playback frequency. When the approaching bats were challenged by an abrupt change in the playback stimulus, they responded by shifting their call frequencies upwards, away from the playback. Interestingly, even bats initially calling below the playback's frequency shifted their frequencies upwards, ‘jumping’ over the playback frequency. These spectral shifts in the bats' calls occurred often within less than 200 ms, in the first echolocation call emitted after the stimulus switch—suggesting that rapid jamming avoidance is important for the bat. PMID:17254989

  10. RNA genetics

    SciTech Connect

    Domingo, E. ); Holland, J.J. . Dept. of Biology); Ahlquist, P. . Dept. of Plant Pathology)

    1988-01-01

    This book contains the proceedings on RNA gentics: Variability of RNA genomes, Volume III. Topics covered include: High error rate, population equilibrium, and evolution of RNA replication systems; Influenza viruses; High rate of nutation and evolution; and Sequence space and quasi species distribution.

  11. RNA genetics

    SciTech Connect

    Domingo, E. ); Holland, J.J. . Dept. of Biology); Ahlquist, P. . Dept. of Plant Pathology)

    1988-01-01

    This book contains the proceedings on RNA genetics: RNA-directed virus replication Volume 1. Topics covered include: Replication of the poliovirus genome; Influenza viral RNA transcription and replication; and Relication of the reoviridal: Information derived from gene cloning and expression.

  12. Telomerase RNA stem terminus element affects template boundary element function, telomere sequence, and shelterin binding.

    PubMed

    Webb, Christopher J; Zakian, Virginia A

    2015-09-08

    The stem terminus element (STE), which was discovered 13 y ago in human telomerase RNA, is required for telomerase activity, yet its mode of action is unknown. We report that the Schizosaccharomyces pombe telomerase RNA, TER1 (telomerase RNA 1), also contains a STE, which is essential for telomere maintenance. Cells expressing a partial loss-of-function TER1 STE allele maintained short stable telomeres by a recombination-independent mechanism. Remarkably, the mutant telomere sequence was different from that of wild-type cells. Generation of the altered sequence is explained by reverse transcription into the template boundary element, demonstrating that the STE helps maintain template boundary element function. The altered telomeres bound less Pot1 (protection of telomeres 1) and Taz1 (telomere-associated in Schizosaccharomyces pombe 1) in vivo. Thus, the S. pombe STE, although distant from the template, ensures proper telomere sequence, which in turn promotes proper assembly of the shelterin complex.

  13. Obstacle-avoiding navigation system

    DOEpatents

    Borenstein, Johann; Koren, Yoram; Levine, Simon P.

    1991-01-01

    A system for guiding an autonomous or semi-autonomous vehicle through a field of operation having obstacles thereon to be avoided employs a memory for containing data which defines an array of grid cells which correspond to respective subfields in the field of operation of the vehicle. Each grid cell in the memory contains a value which is indicative of the likelihood, or probability, that an obstacle is present in the respectively associated subfield. The values in the grid cells are incremented individually in response to each scan of the subfields, and precomputation and use of a look-up table avoids complex trigonometric functions. A further array of grid cells is fixed with respect to the vehicle form a conceptual active window which overlies the incremented grid cells. Thus, when the cells in the active window overly grid cell having values which are indicative of the presence of obstacles, the value therein is used as a multiplier of the precomputed vectorial values. The resulting plurality of vectorial values are summed vectorially in one embodiment of the invention to produce a virtual composite repulsive vector which is then summed vectorially with a target-directed vector for producing a resultant vector for guiding the vehicle. In an alternative embodiment, a plurality of vectors surrounding the vehicle are computed, each having a value corresponding to obstacle density. In such an embodiment, target location information is used to select between alternative directions of travel having low associated obstacle densities.

  14. Adaptive Avoidance of Reef Noise

    PubMed Central

    Simpson, Stephen D.; Radford, Andrew N.; Tickle, Edward J.; Meekan, Mark G.; Jeffs, Andrew G.

    2011-01-01

    Auditory information is widely used throughout the animal kingdom in both terrestrial and aquatic environments. Some marine species are dependent on reefs for adult survival and reproduction, and are known to use reef noise to guide orientation towards suitable habitat. Many others that forage in food-rich inshore waters would, however, benefit from avoiding the high density of predators resident on reefs, but nothing is known about whether acoustic cues are used in this context. By analysing a sample of nearly 700,000 crustaceans, caught during experimental playbacks in light traps in the Great Barrier Reef lagoon, we demonstrate an auditory capability in a broad suite of previously neglected taxa, and provide the first evidence in any marine organisms that reef noise can act as a deterrent. In contrast to the larvae of species that require reef habitat for future success, which showed an attraction to broadcasted reef noise, taxa with a pelagic or nocturnally emergent lifestyle actively avoided it. Our results suggest that a far greater range of invertebrate taxa than previously thought can respond to acoustic cues, emphasising yet further the potential negative impact of globally increasing levels of underwater anthropogenic noise. PMID:21326604

  15. Adaptive avoidance of reef noise.

    PubMed

    Simpson, Stephen D; Radford, Andrew N; Tickle, Edward J; Meekan, Mark G; Jeffs, Andrew G

    2011-02-04

    Auditory information is widely used throughout the animal kingdom in both terrestrial and aquatic environments. Some marine species are dependent on reefs for adult survival and reproduction, and are known to use reef noise to guide orientation towards suitable habitat. Many others that forage in food-rich inshore waters would, however, benefit from avoiding the high density of predators resident on reefs, but nothing is known about whether acoustic cues are used in this context. By analysing a sample of nearly 700,000 crustaceans, caught during experimental playbacks in light traps in the Great Barrier Reef lagoon, we demonstrate an auditory capability in a broad suite of previously neglected taxa, and provide the first evidence in any marine organisms that reef noise can act as a deterrent. In contrast to the larvae of species that require reef habitat for future success, which showed an attraction to broadcasted reef noise, taxa with a pelagic or nocturnally emergent lifestyle actively avoided it. Our results suggest that a far greater range of invertebrate taxa than previously thought can respond to acoustic cues, emphasising yet further the potential negative impact of globally increasing levels of underwater anthropogenic noise.

  16. Threshold-avoiding proteomics pipeline.

    PubMed

    Suits, Frank; Hoekman, Berend; Rosenling, Therese; Bischoff, Rainer; Horvatovich, Peter

    2011-10-15

    We present a new proteomics analysis pipeline focused on maximizing the dynamic range of detected molecules in liquid chromatography-mass spectrometry (LC-MS) data and accurately quantifying low-abundance peaks to identify those with biological relevance. Although there has been much work to improve the quality of data derived from LC-MS instruments, the goal of this study was to extend the dynamic range of analyzed compounds by making full use of the information available within each data set and across multiple related chromatograms in an experiment. Our aim was to distinguish low-abundance signal peaks from noise by noting their coherent behavior across multiple data sets, and central to this is the need to delay the culling of noise peaks until the final peak-matching stage of the pipeline, when peaks from a single sample appear in the context of all others. The application of thresholds that might discard signal peaks early is thereby avoided, hence the name TAPP: threshold-avoiding proteomics pipeline. TAPP focuses on quantitative low-level processing of raw LC-MS data and includes novel preprocessing, peak detection, time alignment, and cluster-based matching. We demonstrate the performance of TAPP on biologically relevant sample data consisting of porcine cerebrospinal fluid spiked over a wide range of concentrations with horse heart cytochrome c.

  17. The avoidable costs of population.

    PubMed

    Sadie, J L

    1987-07-01

    The social and economic consequences of current demographic trends in South Africa are analyzed using the concept of avoidable costs. The author presents projections of the population up to the year 2000 organized under four major categories: executive and managerial; professional; semi-skilled; and the unskilled, peasants, underemployed, and very poor. Comparisons are made with projections for the same four categories for Canada to show the problems faced by South Africa, in that relatively small growth in the first two classes is contrasted to massive growth in the other, and particularly the least privileged, classes. Consideration is given to the implications for the provision of schools and for the labor force. The author concludes that "if a problem is to be tackled at its roots it is to the control of human numbers that our attention is to be directed."

  18. Bone tumor mimics: avoiding misdiagnosis.

    PubMed

    Gould, C Frank; Ly, Justin Q; Lattin, Grant E; Beall, Douglas P; Sutcliffe, Joseph B

    2007-01-01

    Whether discovered incidentally or as part of a focused diagnostic evaluation, the finding of a benign osseous lesion that has radiologic features resembling a bone tumor is not uncommon. Some of the more common benign and nonneoplastic entities that can sometimes be confused with tumors are the following: cortical desmoid, Brodie abscess, synovial herniation pit, pseudocyst, enostosis, intraosseous ganglion cyst, fibrous dysplasia, stress fracture, avulsion fracture (healing stage), bone infarct, myositis ossificans, brown tumor, and subchondral cyst. Accurate diagnosis and management of these lesions require a basic understanding of their epidemiology, clinical presentations, anatomic distributions, imaging features, differential considerations, and therapeutic options. This in-depth review of 13 potential bone tumor mimics will assist the radiologist in correctly identifying these benign lesions and in avoiding misdiagnosis and related morbidity. This review will also aid the radiologist in making appropriate recommendations to the referring physician for management or further imaging.

  19. Autonomous hazard detection and avoidance

    NASA Technical Reports Server (NTRS)

    Pien, Homer

    1992-01-01

    During GFY 91, Draper Laboratory was awarded a task by NASA-JSC under contract number NAS9-18426 to study and evaluate the potential for achieving safe autonomous landings on Mars using an on-board autonomous hazard detection and avoidance (AHDA) system. This report describes the results of that study. The AHDA task had four objectives: to demonstrate, via a closed-loop simulation, the ability to autonomously select safe landing sites and the ability to maneuver to the selected site; to identify key issues in the development of AHDA systems; to produce strawman designs for AHDA sensors and algorithms; and to perform initial trade studies leading to better understanding of the effect of sensor/terrain/viewing parameters on AHDA algorithm performance. This report summarizes the progress made during the first year, with primary emphasis on describing the tools developed for simulating a closed-loop AHDA landing. Some cursory performance evaluation results are also presented.

  20. ACAT Ground Collision Avoidance Flight Tests Over

    NASA Video Gallery

    NASA's Dryden Flight Research Center has concluded flight tests of an Automatic Ground Collision Avoidance System (Auto GCAS) under the joint U.S. Air Force/NASA F-16D Automatic Collision Avoidance...

  1. Obstacle avoidance sonar for submarines

    NASA Astrophysics Data System (ADS)

    Dugas, Albert C.; Webman, Kenneth M.

    2002-05-01

    The Advanced Mine Detection Sonar (AMDS) system was designed to operate in poor environments with high biological and/or shallow-water boundary conditions. It provides increased capability for active detection of volume, close-tethered, and bottom mines, as well as submarine and surface target active/passive detection for ASW and collision avoidance. It also provides bottom topography mapping capability for precise submarine navigation in uncharted littoral waters. It accomplishes this by using advanced processing techniques with extremely narrow beamwidths. The receive array consists of 36 modules arranged in a 15-ft-diameter semicircle at the bottom of the submarine sonar dome to form a chin-mounted array. Each module consists of 40 piezoelectric rubber elements. The modules provide the necessary signal conditioning to the element data prior to signal transmission (uplink) through the hull. The elements are amplified, filtered, converted to digital signals by an A/D converter, and multiplexed prior to uplink to the inboard receiver. Each module also has a downlink over which it receives synchronization and mode/gain control. Uplink and downlink transmission is done using fiberoptic telemetry. AMDS was installed on the USS Asheville. The high-frequency chin array for Virginia class submarines is based on the Asheville design.

  2. RNA. Introduction.

    PubMed

    Bao, Marie Z; Kruger, Robert P; Rivas, Fabiola; Smith, Orla; Szewczak, Lara

    2009-02-20

    Two scientists walk into a bar. After a pint and an exchange of pleasantries, one says to the other, "Where do you come from? Scientifically, I mean." The queried scientist responds, "Out of the RNA world." "Don't we all," the asker responds chuckling. Fifteen years ago, the joke would have been made with a nod to the notion that life arose from an RNA-based precursor, the so-called "RNA world." Yet had this conversation happened last week, the scientists would also be grinning in appreciation of the extent to which contemporary cellular biology is steeped in all things RNA. Ours is truly an RNA world.In this year's special review issue, the Cell editorial team has brought together articles focused on RNA in the modern world, providing perspectives on classical and emerging areas of inquiry. We extend our thanks to the many distinguished experts who contributed their time and effort as authors and reviewers to make the issue informative, thought-provoking, and timely. We hope that this collection of articles, written as we stand on the verge of a new wave of RNA biology, edifies and inspires by revealing the inner workings of these versatile molecules and by highlighting the next key questions that need to be addressed as we strive to understand the full functional scope of RNA in cells.

  3. Avoidance of aluminum by rainbow trout

    SciTech Connect

    Exley, C.

    2000-04-01

    Aluminum is the principal toxicant in fish in acid waters. The ability to avoid Al, particularly at low concentrations, would confer a considerable ecological advantage, but previous research into avoidance of Al has produced mixed results. The author used a cylindrical perspex tank, 150 cm in length, to study avoidance of Al by rainbow trout fry. The fish avoided Al, and their response was dependent on pH. Avoidance that was demonstrated at pHs of 5.00, 5.50, 5.50, and 5.75 was abolished at a pH of 6.00. Fry avoided very low Al concentrations being sensitive to [Al] > 1.00 {micro}mol L{sup {minus}1} at a pH of 5.00. This unequivocal demonstration of avoidance by rainbow trout fry of Al may have important implications for the ecology of indigenous fish populations in surface waters impacted by acidic deposition.

  4. RNA Research

    NASA Technical Reports Server (NTRS)

    1998-01-01

    It is generally believed that an RNA World existed at an early stage in the history of life. During this early period, RNA molecules are seen to be potentially involved in both catalysis and the storage of genetic information. It is widely believed that this RNA World was extensive and therefore a sophisticated nucleic acid replication machinery would presumably predate the translation machinery which would not be needed until later stages in the development of life. This view of an extended RNA World is not necessarily correct. From the point of view of exobiology, the difference in these two views mainly affects the significance of studies of the extent of catalysis possible by RNA- In either case, the origin of the translation machinery and the principles of RNA evolution remain central problems in exobiology. Translation presents several interrelated themes of inquiry for exobiology. First, it is essential, for understanding the very origin of life, how peptides and eventually proteins might have come to be made on the early Earth in a template directed manner. Second, it is necessary to understand how a machinery of similar complexity to that found in the ribosomes of modem organisms came to exist by the time of the last common ancestor (as detected by 16S RRNA sequence studies). Third, the RNAs that comprise the ribosome are themselves likely of very early origin and studies of their history may be very informative about the nature of the RNA World. Moreover, studies of these RNAs will contribute to a better understanding of the potential roles of RNA in early evolution.

  5. Generalization of socially transmitted and instructed avoidance

    PubMed Central

    Cameron, Gemma; Schlund, Michael W.; Dymond, Simon

    2015-01-01

    Excessive avoidance behavior, in which an instrumental action prevents an upcoming aversive event, is a defining feature of anxiety disorders. Left unchecked, both fear and avoidance of potentially threatening stimuli may generalize to perceptually related stimuli and situations. The behavioral consequences of generalization mean that aversive learning experiences with specific threats may lead to the inference that classes of related stimuli are threatening, potentially dangerous, and need to be avoided, despite differences in physical form. Little is known however about avoidance generalization in humans and the learning pathways by which it may be transmitted. In the present study, we compared two pathways to avoidance—instructions and social observation—on subsequent generalization of avoidance behavior, fear expectancy and physiological arousal. Participants first learned that one cue was a danger cue (conditioned stimulus, CS+) and another was a safety cue (CS−). Groups were then either instructed that a simple avoidance response in the presence of the CS+ cancelled upcoming shock (instructed-learning group) or observed a short movie showing a demonstrator performing the avoidance response to prevent shock (observational-learning group). During generalization testing, danger and safety cues were presented along with generalization stimuli that parametrically varied in perceptual similarity to the CS+. Reinstatement of fear and avoidance was also tested. Findings demonstrate, for the first time, generalization of socially transmitted and instructed avoidance: both groups showed comparable generalization gradients in fear expectancy, avoidance behavior and arousal. Return of fear was evident, suggesting that generalized avoidance remains persistent following extinction testing. The utility of the present paradigm for research on avoidance generalization is discussed. PMID:26150773

  6. Detection And Avoidance Of Obstacles By Helicopters

    NASA Technical Reports Server (NTRS)

    Cheng, Victor H. L.; Sridhar, Banavar

    1992-01-01

    Report discusses problems relevant to control subsystems enabling helicopters on nap-of-the-Earth flight paths to detect and avoid obstacles automatically. Indicates similarities between this and obstacle-avoidance problem of industrial mobile robots. Two approaches extend two-dimensional obstacle-avoidance concept to three dimensions. First involves direct search of three-dimensional range-map data for indications of openings between obstacles. Second involves compression of data into two-dimensional map for path search.

  7. Flight Tests Validate Collision-Avoidance System

    NASA Video Gallery

    Flights tests of a smartphone-assisted automatic ground collision avoidance system at NASA's Dryden Flight Research Center consistently commanded evasive maneuvers when it sensed that the unmanned ...

  8. Integration of Weather Avoidance and Traffic Separation

    NASA Technical Reports Server (NTRS)

    Consiglio, Maria C.; Chamberlain, James P.; Wilson, Sara R.

    2011-01-01

    This paper describes a dynamic convective weather avoidance concept that compensates for weather motion uncertainties; the integration of this weather avoidance concept into a prototype 4-D trajectory-based Airborne Separation Assurance System (ASAS) application; and test results from a batch (non-piloted) simulation of the integrated application with high traffic densities and a dynamic convective weather model. The weather model can simulate a number of pseudo-random hazardous weather patterns, such as slow- or fast-moving cells and opening or closing weather gaps, and also allows for modeling of onboard weather radar limitations in range and azimuth. The weather avoidance concept employs nested "core" and "avoid" polygons around convective weather cells, and the simulations assess the effectiveness of various avoid polygon sizes in the presence of different weather patterns, using traffic scenarios representing approximately two times the current traffic density in en-route airspace. Results from the simulation experiment show that the weather avoidance concept is effective over a wide range of weather patterns and cell speeds. Avoid polygons that are only 2-3 miles larger than their core polygons are sufficient to account for weather uncertainties in almost all cases, and traffic separation performance does not appear to degrade with the addition of weather polygon avoidance. Additional "lessons learned" from the batch simulation study are discussed in the paper, along with insights for improving the weather avoidance concept. Introduction

  9. How Do Speakers Avoid Ambiguous Linguistic Expressions?

    ERIC Educational Resources Information Center

    Ferreira, V.S.; Slevc, L.R.; Rogers, E.S.

    2005-01-01

    Three experiments assessed how speakers avoid linguistically and nonlinguistically ambiguous expressions. Speakers described target objects (a flying mammal, bat) in contexts including foil objects that caused linguistic (a baseball bat) and nonlinguistic (a larger flying mammal) ambiguity. Speakers sometimes avoided linguistic-ambiguity, and they…

  10. Recommendations for Sense and Avoid Policy Compliance

    NASA Technical Reports Server (NTRS)

    2005-01-01

    Since unmanned aircraft do not have a human on board, they need to have a sense and avoid capability that provides an "equivalent level of safety" (ELOS) to manned aircraft. The question then becomes - is sense and avoid ELOS for unmanned aircraft adequate to satisfy the requirements of 14 CFR 91.113? Access 5 has proposed a definition of sense and avoid, but the question remains as to whether any sense and avoid system can comply with 14 CFR 91.113 as currently written. The Access 5 definition of sense and avoid ELOS allows for the development of a sense and avoid system for unmanned aircraft that would comply with 14 CFR 91.113. Compliance is based on sensing and avoiding other traffic at an equivalent level of safety for collision avoidance, as manned aircraft. No changes to Part 91 are necessary, with the possible exception of changing "see" to "sense," or obtaining an interpretation from the FAA General Counsel that "sense" is equivalent to "see."

  11. Population properties affect inbreeding avoidance in moose.

    PubMed

    Herfindal, Ivar; Haanes, Hallvard; Røed, Knut H; Solberg, Erling J; Markussen, Stine S; Heim, Morten; Sæther, Bernt-Erik

    2014-12-01

    Mechanisms reducing inbreeding are thought to have evolved owing to fitness costs of breeding with close relatives. In small and isolated populations, or populations with skewed age- or sex distributions, mate choice becomes limited, and inbreeding avoidance mechanisms ineffective. We used a unique individual-based dataset on moose from a small island in Norway to assess whether inbreeding avoidance was related to population structure and size, expecting inbreeding avoidance to be greater in years with larger populations and even adult sex ratios. The probability that a potential mating event was realized was negatively related to the inbreeding coefficient of the potential offspring, with a stronger relationship in years with a higher proportion or number of males in the population. Thus, adult sex ratio and population size affect the degree of inbreeding avoidance. Consequently, conservation managers should aim for sex ratios that facilitate inbreeding avoidance, especially in small and isolated populations.

  12. Living with food allergy: allergen avoidance.

    PubMed

    Kim, Jennifer S; Sicherer, Scott H

    2011-04-01

    The primary treatment of food allergy is to avoid the culprit foods. This is a complex undertaking that requires education about reading the labels of manufactured products, understanding how to avoid cross-contact with allergens during food preparation, and communicating effectively with persons who are providing allergen-safe meals including relatives and restaurant personnel. Successful avoidance also requires a knowledge of nuances such as appropriate cleaning practices, an understanding of the risks of ingestion compared to skin contact or inhalation, that exposure could occur through unanticipated means such as through sharing utensils or passionate kissing, and that food may be a component of substances that are not ingested such as cosmetics, bath products, vaccines and medications. The authors review the necessary tools of avoidance that physicians and medical practitioners can use to guide their patients through the complexities of food avoidance.

  13. Higher threat avoidance costs reduce avoidance behaviour which in turn promotes fear extinction in humans.

    PubMed

    Rattel, Julina A; Miedl, Stephan F; Blechert, Jens; Wilhelm, Frank H

    2016-12-15

    Theoretical models specifying the underlying mechanisms of the development and maintenance of anxiety and related disorders state that fear responses acquired through classical Pavlovian conditioning are maintained by repeated avoidance behaviour; thus, it is assumed that avoidance prevents fear extinction. The present study investigated behavioural avoidance decisions as a function of avoidance costs in a naturalistic fear conditioning paradigm. Ecologically valid avoidance costs - manipulated between participant groups - were represented via time-delays during a detour in a gamified computer task. After differential acquisitions of shock-expectancy to a predictive conditioned stimulus (CS+), participants underwent extinction where they could either take a risky shortcut, while anticipating shock signaled by the CS+, or choose a costly avoidance option (lengthy detour); thus, they were faced with an approach-avoidance conflict. Groups with higher avoidance costs (longer detours) showed lower proportions of avoiders. Avoiders gave heightened shock-expectancy ratings post-extinction, demonstrating 'protecting from extinction', i.e. failure to extinguish. Moreover, there was an indirect effect of avoidance costs on protection from extinction through avoidance behaviour. No moderating role of trait-anxiety was found. Theoretical implications of avoidance behaviour are discussed, considering the involvement of instrumental learning in the maintenance of fear responses.

  14. When Is Topic Avoidance Unsatisfying? Examining Moderators of the Association between Avoidance and Dissatisfaction

    ERIC Educational Resources Information Center

    Caughlin, John P.; Afifi, Tamara D.

    2004-01-01

    Despite theoretical arguments that avoiding certain topics can be functional, there is consistent evidence that avoiding topics tends to be associated with dissatisfying relationships. This disparity between theory and empirical findings suggests a need to understand better the connection between topic avoidance and relational dissatisfaction. The…

  15. Avoid the Consequences of High Blood Pressure

    MedlinePlus

    ... Aneurysm More Avoid the Consequences of High Blood Pressure Infographic Updated:Oct 31,2016 View a downloadable version of this infographic High Blood Pressure • Home • Get the Facts About HBP • Know Your ...

  16. Sulfa Allergy: Which Medications Should I Avoid?

    MedlinePlus

    ... sulfa allergy. Do I need to avoid certain medications? Answers from James T C Li, M.D., ... drugs: Sulfamethoxazole-trimethoprim (Septra, Bactrim) Erythromycin-sulfisoxazole Other medications that may cause a reaction Other types of ...

  17. When Should a Mother Avoid Breastfeeding?

    MedlinePlus

    ... Address What's this? Submit What's this? Submit Button Breastfeeding Information for Families Breastfeeding Hotline The HHS Office ... Tweet Share Compartir When should a mother avoid breastfeeding? Health professionals agree that human milk provides the ...

  18. Active Collision Avoidance for Planetary Landers

    NASA Technical Reports Server (NTRS)

    Rickman, Doug; Hannan, Mike; Srinivasan, Karthik

    2014-01-01

    Present day robotic missions to other planets require precise, a priori knowledge of the terrain to pre-determine a landing spot that is safe. Landing sites can be miles from the mission objective, or, mission objectives may be tailored to suit landing sites. Future robotic exploration missions should be capable of autonomously identifying a safe landing target within a specified target area selected by mission requirements. Such autonomous landing sites must (1) 'see' the surface, (2) identify a target, and (3) land the vehicle. Recent advances in radar technology have resulted in small, lightweight, low power radars that are used for collision avoidance and cruise control systems in automobiles. Such radar systems can be adapted for use as active hazard avoidance systems for planetary landers. The focus of this CIF proposal is to leverage earlier work on collision avoidance systems for MSFC's Mighty Eagle lander and evaluate the use of automotive radar systems for collision avoidance in planetary landers.

  19. The challenges for scientists in avoiding plagiarism.

    PubMed

    Fisher, E R; Partin, K M

    2014-01-01

    Although it might seem to be a simple task for scientists to avoid plagiarism and thereby an allegation of research misconduct, assessment of trainees in the Responsible Conduct of Research and recent findings from the National Science Foundation Office of Inspector General regarding plagiarism suggests otherwise. Our experiences at a land-grant academic institution in assisting researchers in avoiding plagiarism are described. We provide evidence from a university-wide multi-disciplinary course that understanding how to avoid plagiarism in scientific writing is more difficult than it might appear, and that a failure to learn the rules of appropriate citation may cause dire consequences. We suggest that new strategies to provide training in avoiding plagiarism are required.

  20. The vigilance-avoidance model of avoidant recognition: An ERP study under threat priming.

    PubMed

    Zhai, Jing; Chen, Xu; Ma, Jianling; Yang, Qingqing; Liu, Ying

    2016-12-30

    Our study examined attachment-related electrophysiological differences in recognition using event-related potentials (ERPs) measured during a study-test paradigm after threat priming. We identified ERP correlates of recognition by comparing the ERPs of attachment-related positive and negative images between avoidant and secure attachment orientations. Our results revealed that the distribution of early old/new effects was broader in avoidant individuals than in secure individuals, and an early parietal old/new effect was observed in avoidant individuals, which reflected their implicit memory. The late old/new effect was found only in secure individuals when evoked by negative pictures, and was not observed in avoidant individuals. The results suggest that avoidant individuals adopt the "vigilance-avoidance" dual-process model to recognize both positive and negative attachment-related stimuli and carry out preferential familiarity matching at the automatic level and avoidant retrieval at the controlled-processing level.

  1. Singularity avoidance and time in quantum gravity

    SciTech Connect

    Kreienbuehl, Andreas

    2009-06-15

    We consider the quantization of a Friedmann-Robertson-Walker universe. We derive a reduced square root Hamiltonian by choosing the scale factor as time variable and quantize the theory using Pauli matrices a la Dirac. From the resulting spinor equation we show that there is no semiclassical wave packet that avoids the big bang singularity. Our work raises the question concerning the relationship between the choice of time and singularity avoidance.

  2. Engineering Structurally Interacting RNA (sxRNA).

    PubMed

    Doyle, Francis; Lapsia, Sameer; Spadaro, Salvatore; Wurz, Zachary E; Bhaduri-McIntosh, Sumita; Tenenbaum, Scott A

    2017-03-28

    RNA-based three-way junctions (3WJs) are naturally occurring structures found in many functional RNA molecules including rRNA, tRNA, snRNA and ribozymes. 3WJs are typically characterized as resulting from an RNA molecule folding back on itself in cis but could also form in trans when one RNA, for instance a microRNA binds to a second structured RNA, such as a mRNA. Trans-3WJs can influence the final shape of one or both of the RNA molecules and can thus provide a means for modulating the availability of regulatory motifs including potential protein or microRNA binding sites. Regulatory 3WJs generated in trans represent a newly identified regulatory category that we call structurally interacting RNA or sxRNA for convenience. Here we show that they can be rationally designed using familiar cis-3WJ examples as a guide. We demonstrate that an sxRNA "bait" sequence can be designed to interact with a specific microRNA "trigger" sequence, creating a regulatable RNA-binding protein motif that retains its functional activity. Further, we show that when placed downstream of a coding sequence, sxRNA can be used to switch "ON" translation of that sequence in the presence of the trigger microRNA and the amount of translation corresponded with the amount of microRNA present.

  3. Engineering Structurally Interacting RNA (sxRNA)

    PubMed Central

    Doyle, Francis; Lapsia, Sameer; Spadaro, Salvatore; Wurz, Zachary E.; Bhaduri-McIntosh, Sumita; Tenenbaum, Scott A.

    2017-01-01

    RNA-based three-way junctions (3WJs) are naturally occurring structures found in many functional RNA molecules including rRNA, tRNA, snRNA and ribozymes. 3WJs are typically characterized as resulting from an RNA molecule folding back on itself in cis but could also form in trans when one RNA, for instance a microRNA binds to a second structured RNA, such as a mRNA. Trans-3WJs can influence the final shape of one or both of the RNA molecules and can thus provide a means for modulating the availability of regulatory motifs including potential protein or microRNA binding sites. Regulatory 3WJs generated in trans represent a newly identified regulatory category that we call structurally interacting RNA or sxRNA for convenience. Here we show that they can be rationally designed using familiar cis-3WJ examples as a guide. We demonstrate that an sxRNA “bait” sequence can be designed to interact with a specific microRNA “trigger” sequence, creating a regulatable RNA-binding protein motif that retains its functional activity. Further, we show that when placed downstream of a coding sequence, sxRNA can be used to switch “ON” translation of that sequence in the presence of the trigger microRNA and the amount of translation corresponded with the amount of microRNA present. PMID:28350000

  4. Beyond negligence: avoidability and medical injury compensation.

    PubMed

    Kachalia, Allen B; Mello, Michelle M; Brennan, Troyen A; Studdert, David M

    2008-01-01

    Disenchantment with the tort system and negligence standard in the United States is fueling interest in alternate compensation systems for medical injury. One possibility is experimentation with administrative "health courts," through which specialized adjudicators would utilize neutral experts to render compensability determinations. Compensation would be based not on negligence, but rather on a broader avoidable medical injury (avoidability) standard. Although considerable interest in health courts exists, stakeholders frequently express uncertainty about the meaning and operation of an avoidability standard. Three nations-Sweden, Denmark, and New Zealand-have long operated administrative schemes. We conducted interviews with administrators and stakeholders in these systems. Our goal was to garner lessons on how to operate a health court, and specifically, how to develop and apply alternate compensation criteria such as avoidability. This article reports our findings on the origins and operations of the systems, the evolution of their compensation criteria, and how these criteria are actually applied. We found that all three systems had their primary genesis in ensuring compensation for the injured, as opposed to sanctioning providers. All have abandoned the negligence standard. The Nordic systems use an avoidability standard, principally defined as injury that would not occur in the hands of the best practitioner. Their experience demonstrates that this definition is feasible to apply. New Zealand's recent move to a no-fault system sheds light on the benefits and drawbacks of a variety of compensation standards. Key lessons for successfully applying an alternate standard, such as avoidability, include a strict adherence to national precedent, the use of neutral and experienced experts, and a block on routine transfer of information from compensation investigations to disciplinary authorities. Importantly, all three nations are harnessing their systems' power to

  5. Rape avoidance behavior among Slovak women.

    PubMed

    Prokop, Pavol

    2013-05-28

    Rape has been a recurrent adaptive problem for many species, including humans. Rape is costly to women in terms of disease transmission, partner abandonment, and unwanted pregnancy (among other costs). Therefore, behavioral strategies which allow women to avoid coercive men may have been favored by selection. In line with this evolutionary reasoning, the current research documented that physically stronger women and those in a committed romantic relationship reported more rape avoidance behavior. In addition, virgin women tended to perform more rape avoidance behavior compared with their non-virgin counterparts. Women with high conception risk perceived themselves as physically stronger, which may protect them against a potential rapist. Fear of unwanted pregnancy from rape decreased as age increased, reflecting higher fertility among younger participants. However, older women reported more rape avoidance behavior, which contradicts evolutionary predictions. The results provide some support for evolutionary hypotheses of rape avoidance behavior which suggest that woman's perception of rape is influenced by parental investment and perceived physical condition.

  6. Avoidance of Phycomyces in a controlled environment.

    PubMed Central

    Meyer, P W; Matus, I J; Berg, H C

    1987-01-01

    The sporangiophore of the fungus Phycomyces bends away from nearby objects without ever touching them. It has been thought that these objects act as aerodynamic obstacles that damp random winds, thereby generating asymmetric distributions of a growth-promoting gas emitted by the growth zone. In the interest of testing this hypothesis, we studied avoidance in an environmental chamber in which convection was suppressed by a shallow thermal gradient. We also controlled pressure, temperature, and relative humidity of the air, electrostatic charge, and ambient light. A protocol was established that yielded avoidance rates constant from sporangiophore to sporangiophore to within +/- 10%. We found that avoidance occurred at normal rates in the complete absence of random winds. The rates were smaller at 100% than at lower values of relative humidity, but not by much. Remarkably, at a distance as great as 0.5 mm, avoidance from a 30-micron diam glass fiber (aligned parallel to the sporangiophore) was about the same as that from a planar glass sheet. However, the rate for the fiber fell more rapidly with distance. The rate for the sheet remained nearly constant out to approximately 4 mm. We conclude that avoidance depends either on adsorption by the barrier of a growth-inhibiting substance or emission by the barrier of a growth-promoting substance; it cannot occur by passive reflection. Models that can explain these effects are analyzed in the Appendix. PMID:3567313

  7. Harm avoidance and disability in old age.

    PubMed

    Wilson, Robert S; Buchman, Aron S; Arnold, Steven E; Shah, Raj C; Tang, Yuxiao; Bennett, David A

    2006-01-01

    The relation of personality to disability in old age is not well understood. The authors examined the relation of harm avoidance, a trait indicating a tendency to worry, fear uncertainty, be shy, and tire easily, to disability in a group of 474 older persons without dementia. Participants completed the 35-item Harm Avoidance scale. Disability was assessed with the Rosow-Breslau scale, a self-report measure of physical mobility. Performance-based tests of lower limb functions were also administered from which composite measures of gait, balance, and strength were derived. In a logistic regression model controlled for age, sex, education, and lower limb function, persons with high levels of harm avoidance were nearly three times as likely to report mobility limitations as persons with low levels, and these effects largely reflected fatigability and fear of uncertainty. The association of harm avoidance with disability was not explained or modified by frailty, physical activity, depressive symptoms, neuroticism, extraversion, or cognition. The results suggest that harm avoidance is associated with disability in old age.

  8. Avoidance of hydrolyzed casein by mice

    PubMed Central

    Field, Kristin L.; Kimball, Bruce A.; Mennella, Julie A.; Beauchamp, Gary K.; Bachmanov, Alexander A.

    2008-01-01

    When casein, a milk protein, is hydrolyzed, it renders human foods that contain it (e.g., hypoallergenic infant formula, cheeses) distasteful to many people. This rejection of hydrolyzed casein (HC)-containing foods has recently been found to also occur in a non-human species (deer, Odocoileus spp.). Identifying other animals that avoid HC would facilitate understanding how and why HC-containing food is often rejected. This study determined whether HC-containing food is avoided by Mus musculus and whether consumption patterns were sensitive to testing conditions, specifically food form (powder, pellet or dough) and food access (ad libitum or 1.5 h/day following 6 h of food deprivation). Diets were offered in two-choice tests that paired an HC-containing food with an intact casein-containing alternative at seven protein concentrations (0%–50% w/w). Five experimental groups were tested under different combinations of food form and food access. Three groups (ad lib/powder, ad lib/pellet, and 1.5 h/pellet) avoided the HC diet starting at the 30% protein level. At the 40% and 50% protein levels, all groups showed strong avoidance of HC. Although testing conditions influenced total caloric intake and body weight gain, avoidance of HC at the highest concentrations was robust to the manipulations in experimental conditions. Our study suggests that mice may be a useful model for understanding the mechanisms of HC rejection. PMID:17900635

  9. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance.

    PubMed

    Ramirez, Karol; Shea, Daniel T; McKim, Daniel B; Reader, Brenda F; Sheridan, John F

    2015-05-01

    Psychosocial stress is associated with altered immunity, anxiety and depression. Previously we showed that repeated social defeat (RSD) promoted microglia activation and social avoidance behavior that persisted for 24days after cessation of RSD. The aim of the present study was to determine if imipramine (a tricyclic antidepressant) would reverse RSD-inducedsocial avoidance and ameliorate neuroinflammatory responses. To test this, C57BL/6 mice were divided into treatment groups. One group from RSD and controls received daily injections of imipramine for 24days, following 6 cycles of RSD. Two other groups were treated with saline. RSD mice spent significantly less time in the interaction zone when an aggressor was present in the cage. Administration of imipramine reversed social avoidance behavior, significantly increasing the interaction time, so that it was similar to that of control mice. Moreover, 24days of imipramine treatment in RSD mice significantly decreased stress-induced mRNA levels for IL-6 in brain microglia. Following ex vivo LPS stimulation, microglia from mice exposed to RSD, had higher mRNA expression of IL-6, TNF-α, and IL-1β, and this was reversed by imipramine treatment. In a second experiment, imipramine was added to drinking water confirming the reversal of social avoidant behavior and decrease in mRNA expression of IL-6 in microglia. These data suggest that the antidepressant imipramine may exert its effect, in part, by down-regulating microglial activation.

  10. Is Avoiding an Aversive Outcome Rewarding? Neural Substrates of Avoidance Learning in the Human Brain

    PubMed Central

    Kim, Hackjin; Shimojo, Shinsuke

    2006-01-01

    Avoidance learning poses a challenge for reinforcement-based theories of instrumental conditioning, because once an aversive outcome is successfully avoided an individual may no longer experience extrinsic reinforcement for their behavior. One possible account for this is to propose that avoiding an aversive outcome is in itself a reward, and thus avoidance behavior is positively reinforced on each trial when the aversive outcome is successfully avoided. In the present study we aimed to test this possibility by determining whether avoidance of an aversive outcome recruits the same neural circuitry as that elicited by a reward itself. We scanned 16 human participants with functional MRI while they performed an instrumental choice task, in which on each trial they chose from one of two actions in order to either win money or else avoid losing money. Neural activity in a region previously implicated in encoding stimulus reward value, the medial orbitofrontal cortex, was found to increase, not only following receipt of reward, but also following successful avoidance of an aversive outcome. This neural signal may itself act as an intrinsic reward, thereby serving to reinforce actions during instrumental avoidance. PMID:16802856

  11. Is avoiding an aversive outcome rewarding? Neural substrates of avoidance learning in the human brain.

    PubMed

    Kim, Hackjin; Shimojo, Shinsuke; O'Doherty, John P

    2006-07-01

    Avoidance learning poses a challenge for reinforcement-based theories of instrumental conditioning, because once an aversive outcome is successfully avoided an individual may no longer experience extrinsic reinforcement for their behavior. One possible account for this is to propose that avoiding an aversive outcome is in itself a reward, and thus avoidance behavior is positively reinforced on each trial when the aversive outcome is successfully avoided. In the present study we aimed to test this possibility by determining whether avoidance of an aversive outcome recruits the same neural circuitry as that elicited by a reward itself. We scanned 16 human participants with functional MRI while they performed an instrumental choice task, in which on each trial they chose from one of two actions in order to either win money or else avoid losing money. Neural activity in a region previously implicated in encoding stimulus reward value, the medial orbitofrontal cortex, was found to increase, not only following receipt of reward, but also following successful avoidance of an aversive outcome. This neural signal may itself act as an intrinsic reward, thereby serving to reinforce actions during instrumental avoidance.

  12. Chloroplast avoidance movement reduces photodamage in plants.

    PubMed

    Kasahara, Masahiro; Kagawa, Takatoshi; Oikawa, Kazusato; Suetsugu, Noriyuki; Miyao, Mitsue; Wada, Masamitsu

    When plants are exposed to light levels higher than those required for photosynthesis, reactive oxygen species are generated in the chloroplasts and cause photodamage. This can occur even under natural growth conditions. To mitigate photodamage, plants have developed several protective mechanisms. One is chloroplast avoidance movement, in which chloroplasts move from the cell surface to the side walls of cells under high light conditions, although experimental support is still awaited. Here, using different classes of mutant defective in chloroplast avoidance movement, we show that these mutants are more susceptible to damage in high light than wild-type plants. Damage of the photosynthetic apparatus and subsequent bleaching of leaf colour and necrosis occur faster under high light conditions in the mutants than in wild-type plants. We conclude that chloroplast avoidance movement actually decreases the amount of light absorption by chloroplasts, and might therefore be important to the survival of plants under natural growth conditions.

  13. Learning processes underlying avoidance of negative outcomes.

    PubMed

    Andreatta, Marta; Michelmann, Sebastian; Pauli, Paul; Hewig, Johannes

    2017-04-01

    Successful avoidance of a threatening event may negatively reinforce the behavior due to activation of brain structures involved in reward processing. Here, we further investigated the learning-related properties of avoidance using feedback-related negativity (FRN). The FRN is modulated by violations of an intended outcome (prediction error, PE), that is, the bigger the difference between intended and actual outcome, the larger the FRN amplitude is. Twenty-eight participants underwent an operant conditioning paradigm, in which a behavior (button press) allowed them to avoid a painful electric shock. During two learning blocks, participants could avoid an electric shock in 80% of the trials by pressing one button (avoidance button), or by not pressing another button (punishment button). After learning, participants underwent two test blocks, which were identical to the learning ones except that no shocks were delivered. Participants pressed the avoidance button more often than the punishment button. Importantly, response frequency increased throughout the learning blocks but it did not decrease during the test blocks, indicating impaired extinction and/or habit formation. In line with a PE account, FRN amplitude to negative feedback after correct responses (i.e., unexpected punishment) was significantly larger than to positive feedback (i.e., expected omission of punishment), and it increased throughout the blocks. Highly anxious individuals showed equal FRN amplitudes to negative and positive feedback, suggesting impaired discrimination. These results confirm the role of negative reinforcement in motivating behavior and learning, and reveal important differences between high and low anxious individuals in the processing of prediction errors.

  14. Integrated Collision Avoidance System for Air Vehicle

    NASA Technical Reports Server (NTRS)

    Lin, Ching-Fang (Inventor)

    2013-01-01

    Collision with ground/water/terrain and midair obstacles is one of the common causes of severe aircraft accidents. The various data from the coremicro AHRS/INS/GPS Integration Unit, terrain data base, and object detection sensors are processed to produce collision warning audio/visual messages and collision detection and avoidance of terrain and obstacles through generation of guidance commands in a closed-loop system. The vision sensors provide more information for the Integrated System, such as, terrain recognition and ranging of terrain and obstacles, which plays an important role to the improvement of the Integrated Collision Avoidance System.

  15. Autonomous Landing and Hazard Avoidance Technology (ALHAT)

    NASA Technical Reports Server (NTRS)

    Epp, Chirold

    2007-01-01

    This viewgraph presentation reviews the work towards technology that will result in an autonomous landing on the lunar surface, that will avoid the hazards of lunar landing. In October 2005, the Exploration Systems Mission Directorate at NASA Headquarters assigned the development of new technologies to support the return to the moon. One of these was Autonomous Precision Landing and Hazard Detection and Avoidance Technology now known as ALHAT ALHAT is a lunar descent and landing GNC technology development project led by Johnson Space Center (JSC) with team members from Langley Research Center (LaRC), Jet Propulsion Laboratory (JPL), Draper Laboratories (CSDL) and the Applied Physics Laboratory (APL)

  16. Telerobotics with whole arm collision avoidance

    SciTech Connect

    Wilhelmsen, K.; Strenn, S.

    1993-09-01

    The complexity of teleorbotic operations in a cluttered environment is exacerbated by the need to present collision information to the operator in an understandable fashion. In addition to preventing movements which will cause collisions, a system providing some form of virtual force reflection (VFR) is desirable. With this goal in mind, Lawrence Livermore National Laboratory (LLNL) has installed a kinematically master/slave system and developed a whole arm collision avoidance system which interacts directly with the telerobotic controller. LLNL has also provided a structure to allow for automated upgrades of workcell models and provide collision avoidance even in a dynamically changing workcell.

  17. Spectrophotometric assays for monitoring tRNA aminoacylation and aminoacyl-tRNA hydrolysis reactions.

    PubMed

    First, Eric A; Richardson, Charles J

    2017-01-15

    Aminoacyl-tRNA synthetases play a central role in protein synthesis, catalyzing the attachment of amino acids to their cognate tRNAs. Here, we describe a spectrophotometric assay for tyrosyl-tRNA synthetase in which the Tyr-tRNA product is cleaved, regenerating the tRNA substrate. As tRNA is the limiting substrate in the assay, recycling it substantially increases the sensitivity of the assay while simultaneously reducing its cost. The tRNA aminoacylation reaction is monitored spectrophotometrically by coupling the production of AMP to the conversion of NAD(+) to NADH. We have adapted the tyrosyl-tRNA synthetase assay to monitor: (1) aminoacylation of tRNA by l- or d-tyrosine, (2) cyclodipeptide formation by cyclodipeptide synthases, (3) hydrolysis of d-aminoacyl-tRNAs by d-tyrosyl-tRNA deacylase, and (4) post-transfer editing by aminoacyl-tRNA synthetases. All of these assays are continuous and homogenous, making them amenable for use in high-throughput screens of chemical libraries. In the case of the cyclodipeptide synthase, d-tyrosyl-tRNA deacylase, and post-transfer editing assays, the aminoacyl-tRNAs are generated in situ, avoiding the need to synthesize and purify aminoacyl-tRNA substrates prior to performing the assays. Lastly, we describe how the tyrosyl-tRNA synthetase assay can be adapted to monitor the activity of other aminoacyl-tRNA synthetases and how the approach to regenerating the tRNA substrate can be used to increase the sensitivity and decrease the cost of commercially available aminoacyl-tRNA synthetase assays.

  18. Development of a Measure of Experiential Avoidance: The Multidimensional Experiential Avoidance Questionnaire

    ERIC Educational Resources Information Center

    Gamez, Wakiza; Chmielewski, Michael; Kotov, Roman; Ruggero, Camilo; Watson, David

    2011-01-01

    Experiential avoidance (EA) has been conceptualized as the tendency to avoid negative internal experiences and is an important concept in numerous conceptualizations of psychopathology as well as theories of psychotherapy. Existing measures of EA have either been narrowly defined or demonstrated unsatisfactory internal consistency and/or evidence…

  19. Flight Test Evaluation of AVOID II. (Avionic Observation of Intruder Danger) Collision Avoidance System

    DTIC Science & Technology

    1976-10-13

    31 General Operation Tests ........................................ 31 Altitude Boundary and Altit~ude Discrimination ...Summary ............................... 57 Altitude Zone Discrimination .................................... 61 AVOID I - AVOID II COMPI.TIBILITY...Figure 16. Commad Display Logic. - 33 - NADC-76141 -60 4 ALTITUDE BOUNDARIES AND ALTITUDE DISCRIMINATION TESTS The main objective of these encounters

  20. Pathological Demand Avoidance: Exploring the Behavioural Profile

    ERIC Educational Resources Information Center

    O'Nions, Elizabeth; Viding, Essi; Greven, Corina U; Ronald, Angelica; Happé, Francesca

    2014-01-01

    "Pathological Demand Avoidance" is a term increasingly used by practitioners in the United Kingdom. It was coined to describe a profile of obsessive resistance to everyday demands and requests, with a tendency to resort to "socially manipulative" behaviour, including outrageous or embarrassing acts. Pathological demand…

  1. 47 CFR 74.604 - Interference avoidance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Stations § 74.604 Interference avoidance. (a) (b) Where two or more licensees are assigned a common channel..., including time sharing arrangements, to assure an equitable distribution of available frequencies. (c) For those interference disputes brought to the Commission for resolution, TV broadcast auxiliary...

  2. Perspectives in avoidance-preference bioassays

    SciTech Connect

    Steele, C.W.; Taylor, D.H.; Strickler-Shaw, S.

    1996-12-31

    Although behavioral endpoints are used in hazard assessment, establishment of water quality criteria and assessment of a contaminant`s hazard to aquatic life rely primarily on standard acute and chronic toxicity tests. Sublethal effects of pollutants should, however, be of major concern because more organisms experience sublethal rather than acutely or chronically lethal exposures of contaminants. The avoidance-preference approach to behavioral bioassays is very useful in screening pollutants for which the mechanisms of perception or response are largely unknown. The underlying philosophy of these studies is that an animal which perceives a chemical can be attracted or repulsed by it. No response is frequently assumed to indicate lack of perception. All three responses have broad ecological implications. The authors discuss the conditions required for performing avoidance-preference bioassays, as well as their sensitivities, advantages, and limitations. In this regard, a comparative approach is used in examining the results of avoidance-preference bioassays with zebrafish in two different apparatuses. Finally, they compare the results of avoidance-preference studies with other measures of the behavioral toxicity of lead to tadpoles.

  3. Social Work Education and Avoidable Ignorance

    ERIC Educational Resources Information Center

    Gambrill, Eileen

    2014-01-01

    The quality of professional education affects the quality of services offered to clients. Examples of avoidable ignorance that dilute the quality of education social workers receive are suggested, indicating that we have not been honest and energetic brokers of knowledge and ignorance. Related reasons are suggested, including a reluctance to take…

  4. Disgust as a Disease-Avoidance Mechanism

    ERIC Educational Resources Information Center

    Oaten, Megan; Stevenson, Richard J.; Case, Trevor I.

    2009-01-01

    Many researchers have claimed that the emotion of disgust functions to protect us from disease. Although there have been several discussions of this hypothesis, none have yet reviewed the evidence in its entirety. The authors derive 14 hypotheses from a disease-avoidance account and evaluate the evidence for each, drawing upon research on pathogen…

  5. Teaching Preschool Children to Avoid Poison Hazards

    ERIC Educational Resources Information Center

    Dancho, Kelly A.; Thompson, Rachel H.; Rhoades, Melissa M.

    2008-01-01

    We evaluated the effectiveness of group safety training and in situ feedback and response interruption to teach preschool children to avoid consuming potentially hazardous substances. Three children ingested ambiguous substances during a baited baseline assessment condition and continued to ingest these substances following group safety training.…

  6. Freeze tolerance and avoidance in plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cold acclimation is a multigenic, quantitative trait that involves biochemical and structural changes that effect the physiology of a plant. Mechanisms associated with freeze tolerance or freeze avoidance develop and are lost on an annual cycle. When conducting studies to characterize and determin...

  7. Specific Language Impairment at Adolescence: Avoiding Complexity

    ERIC Educational Resources Information Center

    Tuller, Laurice; Henry, Celia; Sizaret, Eva; Barthez, Marie-Anne

    2012-01-01

    This study explores complex language in adolescents with specific language impairment (SLI) with the aim of finding out how aspects of language characteristic of typical syntactic development after childhood fare and, in particular, whether there is evidence that individuals with SLI avoid using structures whose syntactic derivation involves…

  8. Pathogen evolution under host avoidance plasticity.

    PubMed

    McLeod, David V; Day, Troy

    2015-09-07

    Host resistance consists of defences that limit pathogen burden, and can be classified as either adaptations targeting recovery from infection or those focused upon infection avoidance. Conventional theory treats avoidance as a fixed strategy which does not vary from one interaction to the next. However, there is increasing empirical evidence that many avoidance strategies are triggered by external stimuli, and thus should be treated as phenotypically plastic responses. Here, we consider the implications of avoidance plasticity for host-pathogen coevolution. We uncover a number of predictions challenging current theory. First, in the absence of pathogen trade-offs, plasticity can restrain pathogen evolution; moreover, the pathogen exploits conditions in which the host would otherwise invest less in resistance, causing resistance escalation. Second, when transmission trades off with pathogen-induced mortality, plasticity encourages avirulence, resulting in a superior fitness outcome for both host and pathogen. Third, plasticity ensures the sterilizing effect of pathogens has consequences for pathogen evolution. When pathogens castrate hosts, selection forces them to minimize mortality virulence; moreover, when transmission trades off with sterility alone, resistance plasticity is sufficient to prevent pathogens from evolving to fully castrate.

  9. Doppler micro sense and avoid radar

    NASA Astrophysics Data System (ADS)

    Gorwara, Ashok; Molchanov, Pavlo; Asmolova, Olga

    2015-10-01

    There is a need for small Sense and Avoid (SAA) systems for small and micro Unmanned Aerial Systems (UAS) to avoid collisions with obstacles and other aircraft. The proposed SAA systems will give drones the ability to "see" close up and give them the agility to maneuver through tight areas. Doppler radar is proposed for use in this sense and avoid system because in contrast to optical or infrared (IR) systems Doppler can work in more harsh conditions such as at dusk, and in rain and snow. And in contrast to ultrasound based systems, Doppler can better sense small sized obstacles such as wires and it can provide a sensing range from a few inches to several miles. An SAA systems comprised of Doppler radar modules and an array of directional antennas that are distributed around the perimeter of the drone can cover the entire sky. These modules are designed so that they can provide the direction to the obstacle and simultaneously generate an alarm signal if the obstacle enters within the SAA system's adjustable "Protection Border". The alarm signal alerts the drone's autopilot to automatically initiate an avoidance maneuver. A series of Doppler radar modules with different ranges, angles of view and transmitting power have been designed for drones of different sizes and applications. The proposed Doppler radar micro SAA system has simple circuitry, works from a 5 volt source and has low power consumption. It is light weight, inexpensive and it can be used for a variety of small unmanned aircraft.

  10. Procedural Variation in Behavioral Avoidance Tests

    ERIC Educational Resources Information Center

    Bernstein, Douglas A.; Nietzel, Michael T.

    1973-01-01

    The data on approach to snakes provides srong support for the contention that the behavorial avoidance test is not immune to bias introduced by the operation of situational variables that have usually been allowed to vary in uncontrolled fashion in psychotherapy analogue research. Behavior of test subjects can be influenced not only in terms of…

  11. Organising European technical documentation to avoid duplication.

    PubMed

    Donawa, Maria

    2006-04-01

    The development of comprehensive accurate and well-organised technical documentation that demonstrates compliance with regulatory requirements is a resource-intensive, but critically important activity for medical device manufacturers. This article discusses guidance documents and method of organising technical documentation that may help avoid costly and time-consuming duplication.

  12. The Advocacy or Avoidance of Only Children.

    ERIC Educational Resources Information Center

    Falbo, Toni

    A brief review of the psychological literature on the characteristics of only children is presented in order to determine if the one-child family should be avoided or advocated. The relevant literature is found to be limited in quantity and conceptualization of the only child. Previous literature is divided into three types of study: those with…

  13. Learn to Avoid or Overcome Leadership Obstacles

    ERIC Educational Resources Information Center

    D'Auria, John

    2015-01-01

    Leadership is increasingly recognized as an important factor in moving schools forward, yet we have been relatively random in how we prepare and support them. Four obstacles often block or diminish their effectiveness. Avoiding or overcoming each of these requires an underlying set of skills and knowledge that we believe can be learned and…

  14. An Examination of Avoided Costs in Utah

    SciTech Connect

    Bolinger, Mark; Wiser, Ryan

    2005-01-07

    The Utah Wind Working Group (UWWG) believes there are currently opportunities to encourage wind power development in the state by seeking changes to the avoided cost tariff paid to qualifying facilities (QFs). These opportunities have arisen as a result of a recent renegotiation of Pacificorp's Schedule 37 tariff for wind QFs under 3 MW, as well as an ongoing examination of Pacificorp's Schedule 38 tariff for wind QFs larger than 3 MW. It is expected that decisions made regarding Schedule 38 will also impact Schedule 37. Through the Laboratory Technical Assistance Program (Lab TAP), the UWWG has requested (through the Utah Energy Office) that LBNL provide technical assistance in determining whether an alternative method of calculating avoided costs that has been officially adopted in Idaho would lead to higher QF payments in Utah, and to discuss the pros and cons of this method relative to the methodology recently adopted under Schedule 37 in Utah. To accomplish this scope of work, I begin by summarizing the current method of calculating avoided costs in Utah (per Schedule 37) and Idaho (the ''surrogate avoided resource'' or SAR method). I then compare the two methods both qualitatively and quantitatively. Next I present Pacificorp's four main objections to the use of the SAR method, and discuss the reasonableness of each objection. Finally, I conclude with a few other potential considerations that might add value to wind QFs in Utah.

  15. Avoiding Mathematics Trauma: Alternative Teaching Methods.

    ERIC Educational Resources Information Center

    Ufuktepe, Unal; Ozel, Claire Thomas

    Children in primary education often encounter mathematics having picked up a general fear of mathematics from the society around them. This results in lack of confidence, avoidance of non-standard thought processes, weakness in problem solving strategies, and other negative consequences. This study offers an alternative approach: presenting…

  16. Affordable MMW aircraft collision avoidance system

    NASA Astrophysics Data System (ADS)

    Almsted, Larry D.; Becker, Robert C.; Zelenka, Richard E.

    1997-06-01

    Collision avoidance is of concern to all aircraft, requiring the detection and identification of hazardous terrain or obstacles in sufficient time for clearance maneuvers. The collision avoidance requirement is even more demanding for helicopters, as their unique capabilities result in extensive operations at low-altitude, near to terrain and other hazardous obstacles. TO augment the pilot's visual collision avoidance abilities, some aircraft are equipped with 'enhanced-vision' systems or terrain collision warning systems. Enhanced-vision systems are typically very large and costly systems that are not very covert and are also difficult to install in a helicopter. The display is typically raw images from infrared or radar sensors, and can require a high degree of pilot interpretation and attention. Terrain collision warning system that rely on stored terrain maps are often of low resolution and accuracy and do not represent hazards to the aircraft placed after map sampling. Such hazards could include aircraft parked on runway, man- made towers or buildings and hills. In this paper, a low cost dual-function scanning pencil-beam, millimeter-wave radar forward sensor is used to determine whether an aircraft's flight path is clear of obstructions. Due to the limited space and weight budget in helicopters, the system is a dual function system that is substituted in place of the existing radar altimeter. The system combines a 35 GHz forward looking obstacle avoidance radar and a 4.3 GHz radar altimeter. The forward looking 35 GHz 3D radar's returns are used to construct a terrain and obstruction database surrounding an aircraft, which is presented to the pilot as a synthetic perspective display. The 35 GHz forward looking radar and the associated display was evaluated in a joint NASA Honeywell flight test program in 1996. The tests were conducted on a NASA/Army test helicopter. The test program clearly demonstrated the systems potential usefulness for collision avoidance.

  17. [Avoidance of patient-prosthesis mismatch].

    PubMed

    Sakamoto, Y; Hashimoto, K

    2006-04-01

    To minimize the incidence of patient-prosthesis mismatch (PPM), we have routinely adopted aortic root enlargement to avoid PPM for patients with small aortic annulus. The aim of this study was to review our strategy of avoiding PPM. The Carpentier-Edwards Perimount (CEP) valves were implanted in 53 patients who were mostly aged over 65 and the St. Jude Medical (SJM) mechanical valves were used in 128 patients aged under 65. A standard 21-mm SJM valve was used in only 3 patients and no 19-mm valves were employed. However, 19-mm CEP valves were used in 12 patients with a small body surface area (1.43 +/- 0.14 m2). Of these, 26 patients (14.4%) who had a small aortic annulus and 24 patients aged under 65 underwent aortic root enlargement. No patient receiving an SJM valve had an projected indexed effective orifice area (EOAI) < or = 0.85 cm2/m2 because of performing aortic valve replacement (AVR) with annular enlargement and only 2 (3.8%) out of 53 patients receiving CEP valves developed PPM. Consequently, the prevalence of PPM was 1.1% in this series. The prevalence of PPM was low in patients over 65 years old with a relatively small body size who received bioprosthetic valves. A pericardial bioprosthesis was considered to be an appropriate valve in older population with regard to avoiding PPM. In patients under 65 years old with a small annulus, the first choice for avoiding PPM is aortic annular enlargement, which may be avoided by high performance mechanical valves with larger EOA.

  18. GEO Collision Avoidance using a Service Vehicle

    NASA Astrophysics Data System (ADS)

    Duncan, M.; Concha, M.

    2013-09-01

    Space Situational Awareness (SSA) is defined as the knowledge and characterization of all aspects of space. SSA is now a fundamental and critical component of space operations. The increased dependence on our space assets has in turn lead to a greater need for accurate, near real-time knowledge of all space activities. Key areas of SSA include improved tracking of small objects, determining the intent of maneuvering spacecraft, identifying all potential high risk conjunction events, and leveraging non-traditional sensors in support of the SSA mission. As the size of the space object population grows, the number of collision avoidance maneuvers grows. Moreover, as the SSA mission evolves to near real-time assessment and analysis, the need for new, more sophisticated collision avoidance methods are required. This paper demonstrates the utility of using a service vehicle to perform collision avoidance maneuver for GEO satellites. We present the planning and execution details required to successfully execute a maneuver; given the traditional conjunction analysis timelines. Various operational constraints and scenarios are considered as part of the demonstration. Development of the collision avoidance strategy is created using SpaceNav's collision risk management tool suite. This study aims to determine the agility required of any proposed servicing capability to provide collision avoidance within traditional conjunction analysis and collision avoidance operations timelines. Key trades and analysis items are given to be: 1. How do we fuse the spacecraft state data with the tracking data collected from the proximity sensor that resides on the servicing spacecraft? 2. How do we deal with the possibility that the collision threat for the event may change as the time to close approach is reduced? 3. Perform trade space of maneuver/thrust time versus achievable change in the spacecraft's orbit. 4. Perform trade space of proximity of service vehicle to spacecraft versus time

  19. RAID: a comprehensive resource for human RNA-associated (RNA-RNA/RNA-protein) interaction.

    PubMed

    Zhang, Xiaomeng; Wu, Deng; Chen, Liqun; Li, Xiang; Yang, Jinxurong; Fan, Dandan; Dong, Tingting; Liu, Mingyue; Tan, Puwen; Xu, Jintian; Yi, Ying; Wang, Yuting; Zou, Hua; Hu, Yongfei; Fan, Kaili; Kang, Juanjuan; Huang, Yan; Miao, Zhengqiang; Bi, Miaoman; Jin, Nana; Li, Kongning; Li, Xia; Xu, Jianzhen; Wang, Dong

    2014-07-01

    Transcriptomic analyses have revealed an unexpected complexity in the eukaryote transcriptome, which includes not only protein-coding transcripts but also an expanding catalog of noncoding RNAs (ncRNAs). Diverse coding and noncoding RNAs (ncRNAs) perform functions through interaction with each other in various cellular processes. In this project, we have developed RAID (http://www.rna-society.org/raid), an RNA-associated (RNA-RNA/RNA-protein) interaction database. RAID intends to provide the scientific community with all-in-one resources for efficient browsing and extraction of the RNA-associated interactions in human. This version of RAID contains more than 6100 RNA-associated interactions obtained by manually reviewing more than 2100 published papers, including 4493 RNA-RNA interactions and 1619 RNA-protein interactions. Each entry contains detailed information on an RNA-associated interaction, including RAID ID, RNA/protein symbol, RNA/protein categories, validated method, expressing tissue, literature references (Pubmed IDs), and detailed functional description. Users can query, browse, analyze, and manipulate RNA-associated (RNA-RNA/RNA-protein) interaction. RAID provides a comprehensive resource of human RNA-associated (RNA-RNA/RNA-protein) interaction network. Furthermore, this resource will help in uncovering the generic organizing principles of cellular function network.

  20. Converging evidence of social avoidant behavior in schizophrenia from two approach-avoidance tasks.

    PubMed

    de la Asuncion, Javier; Docx, Lise; Sabbe, Bernard; Morrens, Manuel; de Bruijn, Ellen R A

    2015-10-01

    Many people with schizophrenia suffer from social impairments characterized by active social avoidance, which is related to social phobia common in schizophrenia, while motivational impairments can also result in passive social withdrawal. Although social avoidance is frequently reported in this population, this is the first study to directly compare approach-avoidance tendencies in schizophrenia patients (N = 37) and healthy controls (N = 29). Participants performed two tasks: a computerized approach-avoidance task (AAT) to assess response tendencies toward images of happy and angry faces with direct or averted gaze and a one-to-one personal space test (PST) to gauge more naturalistic approach-avoidance behaviors toward a real person bearing a neutral expression. The AAT results showed that both groups showed faster avoidance responses to angry faces and faster approach responses to happy faces with a direct gaze. Happy faces with averted gaze, however, resulted in faster avoidance responses in the patient group only. On the PST, the patients approached the experimenter less than healthy controls did. This measure of interpersonal distance was positively related to positive symptom severity. Delusions of reference and increased sensitivity to social rejection may explain the patients' avoidance tendencies in response to pictures of happy faces with averted gaze and in the presence of an actual person. The current findings demonstrate the importance of others adopting positive and unambiguous attitudes when interacting with schizophrenia patients to minimize behavioral avoidance patterns, which is particularly relevant for relatives and clinicians whose interactions with the patients are crucial to facilitating treatment and promoting healthy social relationships.

  1. The Feasibility of Avoiding Future Climate Impacts: Results from the AVOID Programmes

    NASA Astrophysics Data System (ADS)

    Lowe, J. A.; Warren, R.; Arnell, N.; Buckle, S.

    2014-12-01

    The AVOID programme and its successor, AVOID2, have focused on answering three core questions: how do we characterise potentially dangerous climate change and impacts, which emissions pathways can avoid at least some of these impacts, and how feasible are the future reductions needed to significantly deviate from a business-as-usual future emissions pathway. The first AVOID project succeeded in providing the UK Government with evidence to inform its position on climate change. A key part of the work involved developing a range of global emissions pathways and estimating and understanding the corresponding global impacts. This made use of a combination of complex general circulation models, simple climate models, pattern-scaling and state-of-the art impacts models. The results characterise the range of avoidable impacts across the globe in several key sectors including river and coastal flooding, cooling and heating energy demand, crop productivity and aspects of biodiversity. The avoided impacts between a scenario compatible with a 4ºC global warming and one with a 2ºC global warming were found to be highly sector dependent and avoided fractions typically ranged between 20% and 70%. A further key aspect was characterising the magnitude of the uncertainty involved, which is found to be very large in some impact sectors although the avoided fraction appears a more robust metric. The AVOID2 programme began in 2014 and will provide results in the run up to the Paris CoP in 2015. This includes new post-IPCC 5th assessment evidence to inform the long-term climate goal, a more comprehensive assessment of the uncertainty ranges of feasible emission pathways compatible with the long-term goal and enhanced estimates of global impacts using the latest generation of impact models and scenarios.

  2. A Cross-chiral RNA Polymerase Ribozyme

    PubMed Central

    Sczepanski, Jonathan T.; Joyce, Gerald F.

    2014-01-01

    Thirty years ago it was shown that the non-enzymatic, template-directed polymerization of activated mononucleotides proceeds readily in a homochiral system, but is severely inhibited by the presence of the opposing enantiomer.1 This finding poses a severe challenge for the spontaneous emergence of RNA-based life, and has led to the suggestion that either RNA was preceded by some other genetic polymer that is not subject to chiral inhibition2 or chiral symmetry was broken through chemical processes prior to the origin of RNA-based life.3,4 Once an RNA enzyme arose that could catalyze the polymerization of RNA, it would have been possible to distinguish among the two enantiomers, enabling RNA replication and RNA-based evolution to occur. It is commonly thought that the earliest RNA polymerase and its substrates would have been of the same handedness, but this is not necessarily the case. Replicating D-and L-RNA molecules may have emerged together, based on the ability of structured RNAs of one handedness to catalyze the templated polymerization of activated mononucleotides of the opposite handedness. Such a cross-chiral RNA polymerase has now been developed using in vitro evolution. The D-RNA enzyme, consisting of 83 nucleotides, catalyzes the joining of L-mono- or oligonucleotide substrates on a complementary L-RNA template, and similarly for the L-enzyme with D-substrates and a D-template. Chiral inhibition is avoided because the 106-fold rate acceleration of the enzyme only pertains to cross-chiral substrates. The enzyme's activity is sufficient to generate full-length copies of its enantiomer through the templated joining of 11 component oligonucleotides. PMID:25363769

  3. UV impacts avoided by the Montreal Protocol.

    PubMed

    Newman, Paul A; McKenzie, Richard

    2011-07-01

    Temporal and geographical variabilities in the future "world expected" UV environment are compared with the "world avoided", which would have occurred without the Montreal Protocol on Substances That Deplete the Ozone Layer and its subsequent amendments and adjustments. Based on calculations of clear-sky UV irradiances, the effects of the Montreal Protocol have been hugely beneficial to avoid the health risks, such as skin cancer, which are associated with high UV, while there is only a small increase in health risks, such as vitamin D deficiency, that are associated with low UV. However, interactions with climate change may lead to changes in cloud and albedo, and possibly behavioural changes that could also be important.

  4. UV Impacts Avoided by the Montreal Protocol

    NASA Technical Reports Server (NTRS)

    Newman, Paul; McKenzie, Richard

    2010-01-01

    Temporal and geographical variabilities in the future "World Expected" UV environment are compared with the "World Avoided", which would have occurred without the Montreal Protocol on protection of the ozone layer and its subsequent amendments and adjustments. Based on calculations of clear-sky UV irradiances, the effects of the Montreal Protocol have been hugely beneficial to avoid the health risks, such as skin cancer, which are associated with high UV, while there is only a small increase in health risks, such as vitamin D deficiency, that are associated with low UV. However, interactions with climate change may lead to changes in cloud and albedo, and possibly behavioural changes which could also be important.

  5. How to avoid deferred-compensation troubles.

    PubMed

    Freeman, Todd I

    2005-06-01

    Executive compensation packages have long included stock options and deferred compensation plans in order to compete for talent. Last year, Congress passed a law in response to the Enron debacle, in which executives were perceived to be protecting their deferred compensation at the expense of employees, creditors, and investors. The new law is designed to protect companies and their shareholders from being raided by the very executives that guided the company to financial ruin. Physicians who are part owners of medical practices need to know about the changes in the law regarding deferred compensation and how to avoid costly tax penalties. This article discusses how the changes affect medical practices as well as steps physician-owned clinics can take to avoid the risk of penalty, such as freezing deferred compensation and creating a new deferred compensation plan.

  6. Threat detection system for intersection collision avoidance

    NASA Astrophysics Data System (ADS)

    Jocoy, Edward H.; Pierowicz, John A.

    1998-01-01

    Calspan SRL Corporation is currently developing an on- vehicle threat detection system for intersection collision avoidance (ICA) as part of its ICA program with the National Highway Transportation Safety Administration. Crash scenarios were previously defined and an on-board radar sensor was designed. This paper describes recent efforts that include the development of a simulation of a multitarget tracker and collision avoidance algorithm used to predict system performance in a variety of target configurations in the various ICA crash scenarios. In addition, a current headway radar was mounted on the Calspan Instrumented Vehicle and in-traffic data were recorded for two limited crash scenarios. Warning functions were developed through the simulation and applied to the recorded data.

  7. Avoiding the money saving rate increase

    SciTech Connect

    Streiter, S.H.

    1982-06-24

    This is the last in a series of three articles discussing problems for utilities that arise in connection with inflation-swollen capital-investment costs. The first dealt with the problem of front-end loading of charges on capital projects and proposed a way of appreciating new plant at the rate of inflation and earning on the appreciated rate base in later years. The second examined methods of arranging the financing of new plant projects to avoid the incurrence of losses in their early years if the rate base is trended as recommended. This article addresses the question of how to avoid the necessity of a rate increase when placing into service new capital equipment whose purpose was to save ratepayers money. The outcome should be a new regulation on accounting. 2 tables.

  8. Airborne Collision Detection and Avoidance for Small UAS Sense and Avoid Systems

    NASA Astrophysics Data System (ADS)

    Sahawneh, Laith Rasmi

    The increasing demand to integrate unmanned aircraft systems (UAS) into the national airspace is motivated by the rapid growth of the UAS industry, especially small UAS weighing less than 55 pounds. Their use however has been limited by the Federal Aviation Administration regulations due to collision risk they pose, safety and regulatory concerns. Therefore, before civil aviation authorities can approve routine UAS flight operations, UAS must be equipped with sense-and-avoid technology comparable to the see-and-avoid requirements for manned aircraft. The sense-and-avoid problem includes several important aspects including regulatory and system-level requirements, design specifications and performance standards, intruder detecting and tracking, collision risk assessment, and finally path planning and collision avoidance. In this dissertation, our primary focus is on developing an collision detection, risk assessment and avoidance framework that is computationally affordable and suitable to run on-board small UAS. To begin with, we address the minimum sensing range for the sense-and-avoid (SAA) system. We present an approximate close form analytical solution to compute the minimum sensing range to safely avoid an imminent collision. The approach is then demonstrated using a radar sensor prototype that achieves the required minimum sensing range. In the area of collision risk assessment and collision prediction, we present two approaches to estimate the collision risk of an encounter scenario. The first is a deterministic approach similar to those been developed for Traffic Alert and Collision Avoidance (TCAS) in manned aviation. We extend the approach to account for uncertainties of state estimates by deriving an analytic expression to propagate the error variance using Taylor series approximation. To address unanticipated intruders maneuvers, we propose an innovative probabilistic approach to quantify likely intruder trajectories and estimate the probability of

  9. Shaping avoidance behavior in restrained monkeys.

    PubMed

    Lockard, J S

    1969-07-01

    Lever-pulling avoidance behavior of 24 monkeys was actively shaped with a manual shock-control box and a closed-circuit TV system. A negative reinforcement procedure was used wherein a periodically occurring body shock was postponed each time the subject moved toward the lever. All subjects were trainable with this method, two-thirds of them in fewer than five, 1- to 2-hr sessions. Negative reinforcement was more effective than a punishment procedure.

  10. ALHAT: Autonomous Landing and Hazard Avoidance Technology

    NASA Technical Reports Server (NTRS)

    Robertson, Edward A.; Carson, John M., III

    2015-01-01

    The ALHAT project was chartered by NASA HQ in 2006 to develop and mature to TRL 6 an autonomous lunar landing GN&C and sensing system for crewed, cargo, and robotic planetary landing vehicles. The multi-center ALHAT team was tasked with providing a system capable of identifying and avoiding surface hazards in real time to enable safe precision landing to within tens of meters of a designated planetary landing site under any lighting conditions.

  11. Prevention and avoidance of congenital malformations.

    PubMed

    Nevin, N C

    1988-06-15

    Many congenital abnormalities do not have either a Mendelian pattern of inheritance or an identifiable chromosome abnormality and are described as 'multifactorial' as it is assumed they are determined by several genes, each with added effects and modified to a greater or lesser extent by environmental factors. They include spina bifida and anencephaly, cleft lip or cleft palate or both, congenital heart defect and congenital dislocation of the hip, and they constitute a major community health problem. Developments in genetics, biochemistry and cytogenetics have presented new approaches to the prevention and avoidance of congenital abnormalities. The approaches available for the avoidance of congenital malformations include the avoidance of harmful environmental factors, the screening of the newborn and early treatment, genetic counselling and antenatal monitoring with selective termination. The prevention of neural-tube defects in 'high risk' mothers can be achieved by periconceptional vitamin supplementation. In Northern Ireland, of 438 fully supplemented women, only 4 (0.98%) infants or fetuses among 407 infants and fetuses examined had a neural-tube defect, whereas of 356 unsupplemented women, 16 (4.7%) infants or fetuses among 337 infants or fetuses examined had a neural-tube defect.

  12. Traffic jam driving with NMV avoidance

    NASA Astrophysics Data System (ADS)

    Milanés, Vicente; Alonso, Luciano; Villagrá, Jorge; Godoy, Jorge; de Pedro, Teresa; Oria, Juan P.

    2012-08-01

    In recent years, the development of advanced driver assistance systems (ADAS) - mainly based on lidar and cameras - has considerably improved the safety of driving in urban environments. These systems provide warning signals for the driver in the case that any unexpected traffic circumstance is detected. The next step is to develop systems capable not only of warning the driver but also of taking over control of the car to avoid a potential collision. In the present communication, a system capable of autonomously avoiding collisions in traffic jam situations is presented. First, a perception system was developed for urban situations—in which not only vehicles have to be considered, but also pedestrians and other non-motor-vehicles (NMV). It comprises a differential global positioning system (DGPS) and wireless communication for vehicle detection, and an ultrasound sensor for NMV detection. Then, the vehicle's actuators - brake and throttle pedals - were modified to permit autonomous control. Finally, a fuzzy logic controller was implemented capable of analyzing the information provided by the perception system and of sending control commands to the vehicle's actuators so as to avoid accidents. The feasibility of the integrated system was tested by mounting it in a commercial vehicle, with the results being encouraging.

  13. Knowing and Avoiding Plagiarism During Scientific Writing

    PubMed Central

    Kumar, P Mohan; Priya, N Swapna; Musalaiah, SVVS; Nagasree, M

    2014-01-01

    Plagiarism has become more common in both dental and medical communities. Most of the writers do not know that plagiarism is a serious problem. Plagiarism can range from simple dishonesty (minor copy paste/any discrepancy) to a more serious problem (major discrepancy/duplication of manuscript) when the authors do cut-copy-paste from the original source without giving adequate credit to the main source. When we search databases like PubMed/MedLine there is a lot of information regarding plagiarism. However, it is still a current topic of interest to all the researchers to know how to avoid plagiarism. It's time to every young researcher to know ethical guidelines while writing any scientific publications. By using one's own ideas, we can write the paper completely without looking at the original source. Specific words from the source can be added by using quotations and citing them which can help in not only supporting your work and amplifying ideas but also avoids plagiarism. It is compulsory to all the authors, reviewers and editors of all the scientific journals to know about the plagiarism and how to avoid it by following ethical guidelines and use of plagiarism detection software while scientific writing. PMID:25364588

  14. Urban water restrictions: Attitudes and avoidance

    NASA Astrophysics Data System (ADS)

    Cooper, Bethany; Burton, Michael; Crase, Lin

    2011-12-01

    In most urban cities across Australia, water restrictions remain the dominant policy mechanism to restrict urban water consumption. The extensive adoption of water restrictions as a means to limit demand, over several years, means that Australian urban water prices have consistently not reflected the opportunity cost of water. Given the generally strong political support for water restrictions and the likelihood that they will persist for some time, there is value in understanding households' attitudes in this context. More specifically, identifying the welfare gains associated with avoiding urban water restrictions entirely would be a nontrivial contribution to our knowledge and offer insights into the benefits of alternative policy responses. This paper describes the results from a contingent valuation study that investigates consumers' willingness to pay to avoid urban water restrictions. Importantly, the research also investigates the influence of cognitive and exogenous dimensions on the utility gain associated with avoiding water restrictions. The results provide insights into the impact of the current policy mechanism on economic welfare.

  15. Acute carbon dioxide avoidance in Caenorhabditis elegans

    PubMed Central

    Hallem, Elissa A.; Sternberg, Paul W.

    2008-01-01

    Carbon dioxide is produced as a by-product of cellular respiration by all aerobic organisms and thus serves for many animals as an important indicator of food, mates, and predators. However, whether free-living terrestrial nematodes such as Caenorhabditis elegans respond to CO2 was unclear. We have demonstrated that adult C. elegans display an acute avoidance response upon exposure to CO2 that is characterized by the cessation of forward movement and the rapid initiation of backward movement. This response is mediated by a cGMP signaling pathway that includes the cGMP-gated heteromeric channel TAX-2/TAX-4. CO2 avoidance is modulated by multiple signaling molecules, including the neuropeptide Y receptor NPR-1 and the calcineurin subunits TAX-6 and CNB-1. Nutritional status also modulates CO2 responsiveness via the insulin and TGFβ signaling pathways. CO2 response is mediated by a neural circuit that includes the BAG neurons, a pair of sensory neurons of previously unknown function. TAX-2/TAX-4 function in the BAG neurons to mediate acute CO2 avoidance. Our results demonstrate that C. elegans senses and responds to CO2 using multiple signaling pathways and a neural network that includes the BAG neurons and that this response is modulated by the physiological state of the worm. PMID:18524955

  16. Avoiding negative outcomes: tracking the mechanisms of avoidance learning in humans during fear conditioning.

    PubMed

    Delgado, Mauricio R; Jou, Rita L; Ledoux, Joseph E; Phelps, Elizabeth A

    2009-01-01

    Previous research across species has shown that the amygdala is critical for learning about aversive outcomes, while the striatum is involved in reward-related processing. Less is known, however, about the role of the amygdala and the striatum in learning how to exert control over emotions and avoid negative outcomes. One potential mechanism for active avoidance of stressful situations is postulated to involve amygdala-striatal interactions. The goal of this study was to investigate the physiological and neural correlates underlying avoidance learning in humans. Specifically, we used a classical conditioning paradigm where three different conditioned stimuli (CS) were presented. One stimulus predicted the delivery of a shock upon stimulus offset (CS+), while another predicted no negative consequences (CS-). A third conditioned cue also predicted delivery of a shock, but participants were instructed that upon seeing this stimulus, they could avoid the shock if they chose the correct action (AV+). After successful learning, participants could then easily terminate the shock during subsequent stimulus presentations (AV-). Physiological responses (as measured by skin conductance responses) confirmed a main effect of conditioning, particularly showing higher arousal responses during pre (AV+) compared to post (AV-) learning of an avoidance response. Consistent with animal models, amygdala-striatal interactions were observed to underlie the acquisition of an avoidance response. These results support a mechanism of active coping with conditioned fear that allows for the control over emotional responses such as fears that can become maladaptive and influence our decision-making.

  17. Avoiding unseen obstacles: Subcortical vision is not sufficient to maintain normal obstacle avoidance behaviour during reaching.

    PubMed

    Ross, Alasdair I; Schenk, Thomas; Billino, Jutta; Macleod, Mary J; Hesse, Constanze

    2016-10-03

    Previous research found that a patient with cortical blindness (homonymous hemianopia) was able to successfully avoid an obstacle placed in his blind field, despite reporting no conscious awareness of it [Striemer, C. L., Chapman, C. S., & Goodale, M. A., 2009, PNAS, 106(37), 15996-16001]. This finding led to the suggestion that dorsal stream areas, that are assumed to mediate obstacle avoidance behaviour, may obtain their visual input primarily from subcortical pathways. Hence, it was suggested that normal obstacle avoidance behaviour can proceed without input from the primary visual cortex. Here we tried to replicate this finding in a group of patients (N = 6) that suffered from highly circumscribed lesions in the occipital lobe (including V1) that spared the subcortical structures that have been associated with action-blindsight. We also tested if obstacle avoidance behaviour differs depending on whether obstacles are placed only in the blind field or in both the blind and intact visual field of the patients simultaneously. As expected, all patients successfully avoided obstacles placed in their intact visual field. However, none of them showed reliable avoidance behaviour - as indicated by adjustments in the hand trajectory in response to obstacle position - for obstacles placed in their blind visual field. The effects were not dependent on whether one or two obstacles were present. These findings suggest that behaviour in complex visuomotor tasks relies on visual input from occipital areas.

  18. RNA as an Enzyme.

    ERIC Educational Resources Information Center

    Cech, Thomas R.

    1986-01-01

    Reviews current findings that explain RNA's function as an enzyme in addition to being an informational molecule. Highlights recent research efforts and notes changes in the information base on RNA activity. Includes models and diagrams of RNA activity. (ML)

  19. Precision Landing and Hazard Avoidance Doman

    NASA Technical Reports Server (NTRS)

    Robertson, Edward A.; Carson, John M., III

    2016-01-01

    The Precision Landing and Hazard Avoidance (PL&HA) domain addresses the development, integration, testing, and spaceflight infusion of sensing, processing, and GN&C functions critical to the success and safety of future human and robotic exploration missions. PL&HA sensors also have applications to other mission events, such as rendezvous and docking. Autonomous PL&HA builds upon the core GN&C capabilities developed to enable soft, controlled landings on the Moon, Mars, and other solar system bodies. Through the addition of a Terrain Relative Navigation (TRN) function, precision landing within tens of meters of a map-based target is possible. The addition of a 3-D terrain mapping lidar sensor improves the probability of a safe landing via autonomous, real-time Hazard Detection and Avoidance (HDA). PL&HA significantly improves the probability of mission success and enhances access to sites of scientific interest located in challenging terrain. PL&HA can also utilize external navigation aids, such as navigation satellites and surface beacons. Advanced Lidar Sensors High precision ranging, velocimetry, and 3-D terrain mapping Terrain Relative Navigation (TRN) TRN compares onboard reconnaissance data with real-time terrain imaging data to update the S/C position estimate Hazard Detection and Avoidance (HDA) Generates a high-resolution, 3-D terrain map in real-time during the approach trajectory to identify safe landing targets Inertial Navigation During Terminal Descent High precision surface relative sensors enable accurate inertial navigation during terminal descent and a tightly controlled touchdown within meters of the selected safe landing target.

  20. Avoidance learning: a review of theoretical models and recent developments

    PubMed Central

    Krypotos, Angelos-Miltiadis; Effting, Marieke; Kindt, Merel; Beckers, Tom

    2015-01-01

    Avoidance is a key characteristic of adaptive and maladaptive fear. Here, we review past and contemporary theories of avoidance learning. Based on the theories, experimental findings and clinical observations reviewed, we distill key principles of how adaptive and maladaptive avoidance behavior is acquired and maintained. We highlight clinical implications of avoidance learning theories and describe intervention strategies that could reduce maladaptive avoidance and prevent its return. We end with a brief overview of recent developments and avenues for further research. PMID:26257618

  1. Avoiding harm in peritoneal dialysis patients.

    PubMed

    Bender, Filitsa H

    2012-05-01

    This review is focused on minimizing complications and avoiding harm in peritoneal dialysis (PD) patients. Issues related to planning for PD are covered first, with emphasis on PD versus hemodialysis outcomes. Catheter types and insertion techniques are described next, including relevant recommendations by the International Society for Peritoneal Dialysis. A brief review of both noninfectious and infectious complications follows, with emphasis on cardiovascular and metabolic complications. Finally, recommendations for preventing PD-related infections are provided. In conclusion, with proper catheter insertion technique, good training, and attention to detail during the tenure in PD, excellent outcomes can be obtained in a well-informed motivated patient.

  2. Avoiding plagiarism: guidance for nursing students.

    PubMed

    Price, Bob

    The pressures of study, diversity of source materials, past assumptions relating to good writing practice, ambiguous writing guidance on best practice and students' insecurity about their reasoning ability, can lead to plagiarism. With the use of source checking software, there is an increased chance that plagiarised work will be identified and investigated, and penalties given. In extreme cases, plagiarised work may be reported to the Nursing and Midwifery Council and professional as well as academic penalties may apply. This article provides information on how students can avoid plagiarism when preparing their coursework for submission.

  3. Debate: Albumin administration should not be avoided

    PubMed Central

    Allison, Simon P; Lobo, Dileep N

    2000-01-01

    The recent Cochrane report on albumin administration is analysed and criticised on the grounds of clinical methodology, content and interpretation. Although it is naïve and illogical to treat hypoalbuminaemia with albumin infusions, a more balanced view on the use of albumin for resuscitation in acute hypovolaemia is necessary. Once the acute phase of critical illness is past, interstitial volume is often expanded causing oedema, with a low plasma volume. We argue for the use of salt-poor albumin solutions in this situation and conclude that, on current evidence, the assertion that albumin should be avoided in all situations is irrational and untenable. PMID:11211855

  4. Identifying and Avoiding Bias in Research

    PubMed Central

    Pannucci, Christopher J.; Wilkins, Edwin G.

    2010-01-01

    This narrative review provides an overview on the topic of bias as part of Plastic and Reconstructive Surgery's series of articles on evidence-based medicine. Bias can occur in the planning, data collection, analysis, and publication phases of research. Understanding research bias allows readers to critically and independently review the scientific literature and avoid treatments which are suboptimal or potentially harmful. A thorough understanding of bias and how it affects study results is essential for the practice of evidence-based medicine. PMID:20679844

  5. Avoiding Lawsuits for Wage and Hour Violations.

    PubMed

    Silberman, Cherie L

    2016-01-01

    Due to the highly technical language in the wage and hour laws and regulations, employers often find that they have unknowingly violated the Fair Labor Standards Act (FLSA). This can occur because employers have improperly classified an employee as exempt or because employers do not realize that certain time should be paid in full. Improperly classifying employees as exempt or failing to compensate nonexempt employees for all time worked can lead to costly lawsuits, audits, or enforcement actions by the Wage and Hour Division of the Department of Labor. This article discusses the most common FLSA exemptions and provides best practices to avoid liability under the FLSA.

  6. Collision Avoidance for Airport Traffic Concept Evaluation

    NASA Technical Reports Server (NTRS)

    Jones, Denise R.; Prinzel, Lawrence J., III; Otero, Sharon D.; Barker, Glover D.

    2009-01-01

    An initial Collision Avoidance for Airport Traffic (CAAT) concept for the Terminal Maneuvering Area (TMA) was evaluated in a simulation study at the National Aeronautics and Space Administration (NASA) Langley Research Center. CAAT is being designed to enhance surface situation awareness and provide cockpit alerts of potential conflicts during runway, taxi, and low altitude air-to-air operations. The purpose of the study was to evaluate the initial concept for an aircraft-based method of conflict detection and resolution (CD&R) in the TMA focusing on conflict detection algorithms and alerting display concepts. This paper gives an overview of the CD&R concept, simulation study, and test results.

  7. Collision Avoidance for Airport Traffic Simulation Evaluation

    NASA Technical Reports Server (NTRS)

    Jones, Denise R.; Prinzel, Lawrence J., III; Shelton, Kevin J.; Bailey, Randall E.; Otero, Sharon D.; Barker, Glover D.

    2010-01-01

    A Collision Avoidance for Airport Traffic (CAAT) concept for the airport Terminal Maneuvering Area (TMA) was evaluated in a simulation study at the National Aeronautics and Space Administration (NASA) Langley Research Center. CAAT is being designed to enhance surface situation awareness and provide cockpit alerts of potential conflicts during runway, taxi, and low altitude air-to-air operations. The purpose of the study was to evaluate pilot reaction to conflict events in the TMA near the airport, different alert timings for various scenarios, alerting display concepts, and directive alerting concepts. This paper gives an overview of the conflict detection and resolution (CD&R) concept, simulation study, and test results

  8. Honey bees selectively avoid difficult choices.

    PubMed

    Perry, Clint J; Barron, Andrew B

    2013-11-19

    Human decision-making strategies are strongly influenced by an awareness of certainty or uncertainty (a form of metacognition) to increase the chances of making a right choice. Humans seek more information and defer choosing when they realize they have insufficient information to make an accurate decision, but whether animals are aware of uncertainty is currently highly contentious. To explore this issue, we examined how honey bees (Apis mellifera) responded to a visual discrimination task that varied in difficulty between trials. Free-flying bees were rewarded for a correct choice, punished for an incorrect choice, or could avoid choosing by exiting the trial (opting out). Bees opted out more often on difficult trials, and opting out improved their proportion of successful trials. Bees could also transfer the concept of opting out to a novel task. Our data show that bees selectively avoid difficult tasks they lack the information to solve. This finding has been considered as evidence that nonhuman animals can assess the certainty of a predicted outcome, and bees' performance was comparable to that of primates in a similar paradigm. We discuss whether these behavioral results prove bees react to uncertainty or whether associative mechanisms can explain such findings. To better frame metacognition as an issue for neurobiological investigation, we propose a neurobiological hypothesis of uncertainty monitoring based on the known circuitry of the honey bee brain.

  9. Bleeding Avoidance Strategies: Consensus and Controversy

    PubMed Central

    Dauerman, Harold L.; Rao, Sunil V.; Resnic, Frederic S.; Applegate, Robert J.

    2011-01-01

    Bleeding complications after coronary intervention are associated with prolonged hospitalization, increased hospital costs, patient dissatisfaction, morbidity and one year mortality. Bleeding Avoidance Strategies represent a term incorporating multiple modalities that aim to reduce bleeding and vascular complications after cardiovascular catheterization. Recent improvements in the rates of bleeding complications after invasive cardiovascular procedures suggests that the clinical community has successfully embraced specific strategies and improved patient care in this area. There remains controversy regarding the efficacy, safety and/or practicality of 3 key bleeding avoidance strategies for cardiac catheterization and coronary intervention: procedural (radial artery approach, safezone arteriotomy), pharmacologic (multiple agents) and technological (vascular closure devices) approaches to improved access. In this article, we address areas of consensus with respect to selected modalities in order to define the role of each strategy in current practice. Furthermore, we focus on areas of controversy for selected modalities in order to define key areas warranting cautious clinical approaches and the need for future randomized clinical trials in this area. PMID:21700085

  10. Bleeding avoidance strategies. Consensus and controversy.

    PubMed

    Dauerman, Harold L; Rao, Sunil V; Resnic, Frederic S; Applegate, Robert J

    2011-06-28

    Bleeding complications after coronary intervention are associated with prolonged hospitalization, increased hospital costs, patient dissatisfaction, morbidity, and 1-year mortality. Bleeding avoidance strategies is a term incorporating multiple modalities that aim to reduce bleeding and vascular complications after cardiovascular catheterization. Recent improvements in the rates of bleeding complications after invasive cardiovascular procedures suggest that the clinical community has successfully embraced specific strategies and improved patient care in this area. There remains controversy regarding the efficacy, safety, and/or practicality of 3 key bleeding avoidance strategies for cardiac catheterization and coronary intervention: procedural (radial artery approach, safezone arteriotomy), pharmacological (multiple agents), and technological (vascular closure devices) approaches to improved access. In this paper, we address areas of consensus with respect to selected modalities in order to define the role of each strategy in current practice. Furthermore, we focus on areas of controversy for selected modalities in order to define key areas warranting cautious clinical approaches and the need for future randomized clinical trials in this area.

  11. Avoidance of strobe lights by zooplankton

    USGS Publications Warehouse

    Hamel, Martin J.; Richards, Nathan S.; Brown, Michael L.; Chipps, Steven R.

    2010-01-01

    Underwater strobe lights can influence the behavior and distribution of fishes and are increasingly used as a technique to divert fish away from water intake structures on dams. However, few studies examine how strobe lights may affect organisms other than targeted species. To gain insight on strobe lighting effects on nontarget invertebrates, we investigated whether underwater strobe lights influence zooplankton distributions and abundance in Lake Oahe, South Dakota. Zooplankton were collected using vertical tows at 3 discrete distances from an underwater strobe light to quantify the influence of light intensity on zooplankton density. Samples were collected from 3 different depth ranges (0–10 m, 10–20 m and 20–30 m) at <1 m, 15 m and ⩾100 m distance intervals away from the strobe light. Copepods represented 67.2% and Daphnia spp. represented 23.3% of all zooplankton sampled from 17 August to 15 September 2004. Night time zooplankton densities significantly decreased in surface waters when strobe lights were activated. Copepods exhibited the greatest avoidance patterns, while Daphnia avoidance varied throughout sampling depths. These results indicate that zooplankton display negative phototaxic behavior to strobe lights and that researchers must be cognizant of potential effects to the ecosystem such as altering predator–prey interactions or affecting zooplankton distribution and growth.

  12. Underactuated spacecraft formation reconfiguration with collision avoidance

    NASA Astrophysics Data System (ADS)

    Huang, Xu; Yan, Ye; Zhou, Yang

    2017-02-01

    Underactuated collision-free controllers are proposed in this paper for multiple spacecraft formation reconfiguration in circular orbits with the loss of either the radial or in-track thrust. A nonlinear dynamical model of underactuated formation flying is introduced, which is then linearized about circular orbits for controllability and feasibility analyses on underactuated formation reconfiguration. By using the inherent dynamics coupling of system states, reduced-order sliding mode controllers are then designed for either case to indirectly stabilize the system trajectories to the desired formations. In consideration of the collision-avoidance requirement, the artificial potential function method is then employed to design novel underactuated collision-avoidance maneuvers. Rigorous proof substantiates the capabilities of such maneuvers in preventing collisions even in the absence of radial or in-track thrust. Furthermore, a Lyapunov-based analysis ensures the asymptotic stability of the overall closed-loop system. Numerical simulations are performed in a J2-perturbed environment to verify the validity of the proposed underactuated control schemes for collision-free reconfiguration.

  13. Avoiding humiliations in the clinical encounter

    PubMed Central

    Malterud, Kirsti; Hollnagel, Hanne

    2007-01-01

    Objectives To explore potentials for avoiding humiliations in clinical encounters, especially those that are unintended and unrecognized by the doctor. Furthermore, to examine theoretical foundations of degrading behaviour and identify some concepts that can be used to understand such behaviour in the cultural context of medicine. Finally, these concepts are used to build a model for the clinician in order to prevent humiliation of the patient. Theoretical frame of reference Empirical studies document experiences of humiliation among patients when they see their doctor. Philosophical and sociological analysis can be used to explain the dynamics of unintended degrading behaviour between human beings. Skjervheim, Vetlesen, and Bauman have identified the role of objectivism, distantiation, and indifference in the dynamics of evil acts, pointing to the rules of the cultural system, rather than accusing the individual of bad behaviour. Examining the professional role of the doctor, parallel traits embedded in the medical culture are demonstrated. According to Vetlesen, emotional awareness is necessary for moral perception, which again is necessary for moral performance. Conclusion A better balance between emotions and rationality is needed to avoid humiliations in the clinical encounter. The Awareness Model is presented as a strategy for clinical practice and education, emphasizing the role of the doctor's own emotions. Potentials and pitfalls are discussed. PMID:17497482

  14. Lobeline produces conditioned taste avoidance in rats.

    PubMed

    Harrod, S B; Dwoskin, L P; Bardo, M T

    2004-05-01

    Previous results indicate that pretreatment with lobeline attenuates methamphetamine (METH) self-administration in rats, but not by acting as a substitute reinforcer. Given these findings, it has been suggested that lobeline may serve as a useful pharmacotherapy for psychostimulant abuse. However, because lobeline produces emesis and nausea in humans, the present study examined whether lobeline has direct effects on taste avoidance behavior in rats within the same dose range shown previously to decrease METH self-administration. Two experiments utilized a Pavlovian conditioning procedure to determine if lobeline produces conditioned taste avoidance (CTA) in rats. In Experiments 1 and 2, rats consumed either novel milk or salt solutions, respectively, and within 10 min, were injected with lobeline (0.3-3.0 mg/kg) or METH (0.3-3.0 mg/kg). A single-bottle test conducted 48 h after flavor-drug pairings indicated that the dose of lobeline that reduced METH self-administration in a previous study (i.e., 3.0 mg/kg) also produced reliable CTA for milk and salt solution. These findings suggest a need to develop lobeline analogs that reduce METH self-administration, but do not produce CTA following the consumption of a novel solution.

  15. Radar sensors for intersection collision avoidance

    NASA Astrophysics Data System (ADS)

    Jocoy, Edward H.; Phoel, Wayne G.

    1997-02-01

    On-vehicle sensors for collision avoidance and intelligent cruise control are receiving considerably attention as part of Intelligent Transportation Systems. Most of these sensors are radars and `look' in the direction of the vehicle's headway, that is, in the direction ahead of the vehicle. Calspan SRL Corporation is investigating the use of on- vehicle radar for Intersection Collision Avoidance (ICA). Four crash scenarios are considered and the goal is to design, develop and install a collision warning system in a test vehicle, and conduct both test track and in-traffic experiments. Current efforts include simulations to examine ICA geometry-dependent design parameters and the design of an on-vehicle radar and tracker for threat detection. This paper discusses some of the simulation and radar design efforts. In addition, an available headway radar was modified to scan the wide angles (+/- 90 degree(s)) associated with ICA scenarios. Preliminary proof-of-principal tests are underway as a risk reduction effort. Some initial target detection results are presented.

  16. Avoiding microRNA Function Through Alternative Polyadenylation in Prostate Cancer

    DTIC Science & Technology

    2012-04-01

    target sites. (Translated from eng) Science 320(5883):1643-1647 (in eng). 2. Mangone M, et al. (The landscape of C . elegans 3’UTRs. (Translated from eng...orthologue called maternal- effect sterile 4 (MES-4)22,23. In Caenorhabditis  elegans , MES-4 has been reported to be a dimethylase that is spe - cific for...analysed both the positions of nucleosomes and their modification status within the genomes of humans, C .  elegans , D. melanogaster and mice79–81. In

  17. Avoiding microRNA Function Through Alternative Polyandenylation in Prostate Cancer

    DTIC Science & Technology

    2012-10-01

    analysis showing the majority of altered 3’UTRs exhibit shortening. B. Examples of novel CFIm25 targets. C . Examples of genes unaffected by CFIm25...1643-1647 (in eng). 2. Mangone M, et al. (The landscape of C . elegans 3’UTRs. (Translated from eng) Science 329(5990):432-435 (in eng). 3. Jan CH...maternal- effect sterile 4 (MES-4)22,23. In Caenorhabditis  elegans , MES-4 has been reported to be a dimethylase that is spe - cific for H3K36 (REF. 22

  18. T7-RNA Polymerase

    NASA Technical Reports Server (NTRS)

    1997-01-01

    T7-RNA Polymerase grown on STS-81. Structure-Function Relationships of RNA Polymerase: DNA-dependent RNA polymerase is the key enzyme responsible for the biosynthesis of RNA, a process known as transcription. Principal Investigator's include Dr. Dan Carter, Dr. B.C. Wang, and Dr. John Rose of New Century Pharmaceuticals.

  19. Colostomy closure: how to avoid complications

    PubMed Central

    Levitt, Marc A.; Lawal, Taiwo A.; Peña, Alberto

    2010-01-01

    Purpose Colostomy is an operation frequently performed in pediatric surgery. Despite its benefits, it can produce significant morbidity. In a previous publication we presented our experience with the errors and complications that occurred during cases of colostomy creation. We now have focused in the morbidity related to the colostomy closure. The technical details that might have contributed to the minimal morbidity we experienced are described. Methods The medical records of 649 patients who underwent colostomy closure over a 28-year period were retrospectively reviewed looking for complications following these procedures. Our perioperative protocol for colostomy closure consisted in: clear fluids by mouth and repeated proximal stoma irrigations 24 h prior to the operation. Administration of IV antibiotics during anesthesia induction and continued for 48 h. Meticulous surgical technique that included: packing of the proximal stoma, plastic drape to immobilize the surgical field, careful hemostasis, emphasis in avoiding contamination, cleaning the edge of the stomas to allow a good 2-layer, end-to-end anastomosis with separated long-term absorbable sutures, generous irrigation of the peritoneal cavity and subsequent layers with saline solution, closure by layers to avoid dead space, and avoidance of hematomas. No drains and no nasogastric tubes were used. Oral fluids were started the day after surgery and patients were discharged 48–72 h after the operation. Results The original diagnoses of the patients were: anorectal malformation (583), Hirschsprung’s disease (53), and others (13). 10 patients (1.5%) had complications: 6 had intestinal obstruction (5 due to small bowel adhesions, 1 had temporary delay of the function of the anastomosis due to a severe size discrepancy between proximal and distal stoma with a distal microcolon) and 4 incisional hernias. There were no anastomotic dehiscences or wound infection. There was no bleeding, no anastomotic

  20. Geriatric gynecology: promoting health and avoiding harm.

    PubMed

    Miller, Karen L; Baraldi, Carole A

    2012-11-01

    Age increases vulnerability, commonly accompanied by greater reliance on others and susceptibility to maltreatment. Physiologic processes become less resilient; the potential for harm from medical care increases. Awareness of frailty, functional, social, and potential maltreatment issues enables early referrals to help the patient maintain her independence. Health issues that may impede both gynecologic care and self-sufficiency include sensory deficits, physical disability, and cognitive impairment. Speaking slowly and providing contextual information enhance patient comprehension. Cancer screening depends on life expectancy. Osteoporosis treatment requires managing fall risk. Gynecologic symptoms more likely have multiple contributing factors than one etiology. Incontinence is a particularly complex issue, but invariably includes bladder diary assessment and pelvic floor muscle training. Function and frailty measures best predict perioperative morbidity. Communication with the patient, her family, other providers, and health care organizations is an important frontier in avoiding errors and adverse outcomes.

  1. Communication-avoiding symmetric-indefinite factorization

    SciTech Connect

    Ballard, Grey Malone; Becker, Dulcenia; Demmel, James; Dongarra, Jack; Druinsky, Alex; Peled, Inon; Schwartz, Oded; Toledo, Sivan; Yamazaki, Ichitaro

    2014-11-13

    We describe and analyze a novel symmetric triangular factorization algorithm. The algorithm is essentially a block version of Aasen's triangular tridiagonalization. It factors a dense symmetric matrix A as the product A=PLTLTPT where P is a permutation matrix, L is lower triangular, and T is block tridiagonal and banded. The algorithm is the first symmetric-indefinite communication-avoiding factorization: it performs an asymptotically optimal amount of communication in a two-level memory hierarchy for almost any cache-line size. Adaptations of the algorithm to parallel computers are likely to be communication efficient as well; one such adaptation has been recently published. As a result, the current paper describes the algorithm, proves that it is numerically stable, and proves that it is communication optimal.

  2. Mars rover local navigation and hazard avoidance

    NASA Technical Reports Server (NTRS)

    Wilcox, B. H.; Gennery, D. B.; Mishkin, A. H.

    1989-01-01

    A Mars rover sample return mission has been proposed for the late 1990's. Due to the long speed-of-light delays between earth and Mars, some autonomy on the rover is highly desirable. JPL has been conducting research in two possible modes of rover operation, Computer-Aided Remote Driving and Semiautonomous Navigation. A recently-completed research program used a half-scale testbed vehicle to explore several of the concepts in semiautonomous navigation. A new, full-scale vehicle with all computational and power resources on-board will be used in the coming year to demonstrate relatively fast semiautonomous navigation. The computational and power requirements for Mars rover local navigation and hazard avoidance are discussed.

  3. Unstable avoided crossing in coupled spinor condensates.

    PubMed

    Bernier, Nathan R; Dalla Torre, Emanuele G; Demler, Eugene

    2014-08-08

    We consider the dynamics of a Bose-Einstein condensate with two internal states, coupled through a coherent drive. We focus on a specific quench protocol, in which the sign of the coupling field is suddenly changed. At a mean-field level, the system is transferred from a minimum to a maximum of the coupling energy and can remain dynamically stable, in spite of the development of negative-frequency modes. In the presence of a nonzero detuning between the two states, the "charge" and "spin" modes couple, giving rise to an unstable avoided crossing. This phenomenon is generic to systems with two dispersing modes away from equilibrium and constitutes an example of class-I(o) nonequilibrium pattern formation in quantum systems.

  4. Communication-avoiding symmetric-indefinite factorization

    DOE PAGES

    Ballard, Grey Malone; Becker, Dulcenia; Demmel, James; ...

    2014-11-13

    We describe and analyze a novel symmetric triangular factorization algorithm. The algorithm is essentially a block version of Aasen's triangular tridiagonalization. It factors a dense symmetric matrix A as the product A=PLTLTPT where P is a permutation matrix, L is lower triangular, and T is block tridiagonal and banded. The algorithm is the first symmetric-indefinite communication-avoiding factorization: it performs an asymptotically optimal amount of communication in a two-level memory hierarchy for almost any cache-line size. Adaptations of the algorithm to parallel computers are likely to be communication efficient as well; one such adaptation has been recently published. As a result,more » the current paper describes the algorithm, proves that it is numerically stable, and proves that it is communication optimal.« less

  5. Avoiding potholes on the information highway

    NASA Astrophysics Data System (ADS)

    Pagano, Ralph V.

    1996-03-01

    This paper addresses the underlying legal issues that must be considered prior to engaging in the development of multimedia and Internet applications. The often overlooked topics presented in this paper will show the audience, both developers and end users alike, what is required to help pave the information highway. Topics that are covered are: who owns the software, the developer or the party that commissioned the work; whether the current intellectual property laws create a gap in protection of multimedia software; what are the rights and obligations of the software developer and the commissioner; what is one's exposure to liability and how it can be minimized; and, whether one can be held legally responsible for merely selling multimedia software. Multimedia law is a burgeoning area, and in many ways, our laws have not kept pace with technology. Therefore, those involved with developing multimedia applications must be extremely careful to protect their rights, and to avoid liability.

  6. Avoiding potential problems when selling accounts receivable.

    PubMed

    Ayers, D H; Kincaid, T J

    1996-05-01

    Accounts receivable financing is a potential tool for managing a provider organization's working capital needs. But before entering into a financing agreement, organizations need to consider and take steps to avoid serious problems that can arise from participation in an accounts receivable financing program. For example, the purchaser may cease purchasing the receivables, leaving the organization without funding needed for operations. Or, the financing program may be inordinately complex and unnecessarily costly to the organization. Sometimes the organization itself may fail to comply with the terms of the agreement under which the accounts receivable were sold, thus necessitating that restitution be made to the purchaser or provoking charges of fraud. These potential problems should be addressed as early as possible--before an organization enters into an accounts receivable financing program--in order to minimize time, effort, and expanse and maximize the benefits of the financing agreement.

  7. Avoiding sexual harassment liability in veterinary practices.

    PubMed

    Lacroix, C A; Wilson, J F

    1996-05-15

    Harassment based on gender violates the rule of workplace equality established by Title VII of the Civil Rights Act and enforced by the EEOC. In 1986, the US Supreme Court, in Meritor Savings Bank v Vinson, established the criteria that must be met for a claim of hostile environment sexual harassment to be considered valid. Plaintiffs must show that they were subjected to conduct based on their gender, that it was unwelcome, and that it was severe and pervasive enough to alter their condition of employment, resulting in an abusive working environment. There have been few sexual harassment cases involving veterinary professionals, and it is our goal to help keep the number of filed actions to a minimum. The most effective way to avoid hostile environment sexual harassment claims is to confront the issue openly and to adopt a sexual harassment policy for the practice. When it comes to sexual harassment, an ounce of prevention is unquestionably worth a pound of cure.

  8. Environment - avoiding destructive remediation at DOE sites.

    SciTech Connect

    Whicker, F. W.; MacDonell, M. M.; Hinton, T. G.; Pinder, J. E., III; Habegger, L. J.; Environmental Assessment; usdoe

    2004-03-12

    Public perceptions and regulatory agreements have forced the U.S. Department of Energy (DOE) to spend tens of billions of dollars for environmental cleanup of relatively low levels of contamination of soil and water within the nuclear weapons complex. Much of this costly remediation has caused significant ecological damage, but has not resulted in corresponding reductions in public health risks. This Policy Forum offers a potential remedy involving continued federal control of the larger DOE lands and cleanup criteria based on long-term protection of ecosystems and public health, rather than criteria based on the protection of hypothetical future site residents. Recent DOE policy to avoid unnecessary environmental damage using a risk-based strategy is briefly described.

  9. [UNESCO's bioethical norms to avoid eugenic practices].

    PubMed

    Cruz-Coke, R

    2000-06-01

    The author, member of the UNESCO Bioethics Committee, participated in the preparation of the Universal Declaration about Human Genome and Human Rights, in 1997. The aim of this work is to analyze the initial articles of such Declaration, defining the bioethical principles that defend human dignity, freedom and rights, against the madness of the present biotechnological revolution. The development of genetics for the benefit of mankind will be guaranteed if these principles are honored. Genetic discrimination, reductionism and determinism, are identified by the author as perversions that, if used by biotechnologists, can lead to the rebirth of eugenism and racism, that were condemned by the Code of Nuremberg, in 1947. Investigators must assume their responsibility, respecting the principles of human dignity, the real freedom of research and solidarity among people. This attitude will avoid the use of genetics for purposes other than the welfare of mankind.

  10. NASA satellite helps airliners avoid ozone concentrations

    NASA Technical Reports Server (NTRS)

    1981-01-01

    Results from a test to determine the effectiveness of satellite data for helping airlines avoid heavy concentrations of ozone are reported. Information from the Total Ozone Mapping Spectrometer, aboard the Nimbus-7 was transmitted, for use in meteorological forecast activities. The results show: (1) Total Ozone Mapping Spectrometer profile of total ozone in the atmosphere accurately represents upper air patterns and can be used to locate meteorological activity; (2) route forecasting of highly concentrated ozone is feasible; (3) five research aircraft flights were flown in jet stream regions located by the Total Ozone Mapping Spectrometer to determine winds, temperatures, and air composition. It is shown that the jet stream is coincides with the area of highest total ozone gradient, and low total ozone amounts are found where tropospheric air has been carried along above the tropopause on the anticyclonic side of the subtropical jet stream.

  11. Experiential Avoidance and Technological Addictions in Adolescents

    PubMed Central

    García-Oliva, Carlos; Piqueras, José A.

    2016-01-01

    Background and aims This study focuses on the use of popular information and communication technologies (ICTs) by adolescents: the Internet, mobile phones, and video games. The relationship of ICT use and experiential avoidance (EA), a construct that has emerged as underlying and transdiagnostic to a wide variety of psychological problems, including behavioral addictions, is examined. EA refers to a self-regulatory strategy involving efforts to control or escape from negative stimuli such as thoughts, feelings, or sensations that generate strong distress. This strategy, which may be adaptive in the short term, is problematic if it becomes an inflexible pattern. Thus, the aim of this study was to explore whether EA patterns were associated with addictive or problematic use of ICT in adolescents. Methods A total of 317 students of the Spanish southeast between 12 and 18 years old were recruited to complete a questionnaire that included questions about general use of each ICTs, an experiential avoidance questionnaire, a brief inventory of the Big Five personality traits, and specific questionnaires on problematic use of the Internet, mobile phones, and video games. Results Correlation analysis and linear regression showed that EA largely explained results regarding the addictive use of the Internet, mobile phones, and video games, but not in the same way. As regards gender, boys showed a more problematic use of video games than girls. Concerning personality factors, conscientiousness was related to all addictive behaviors. Discussion and conclusions We conclude that EA is an important construct that should be considered in future models that attempt to explain addictive behaviors. PMID:27363463

  12. Avoiding Braess' Paradox Through Collective Intelligence

    NASA Technical Reports Server (NTRS)

    Wolpert , David H.; Tumer, Kagan

    1999-01-01

    In an Ideal Shortest Path Algorithm (ISPA), at each moment each router in a network sends all of its traffic down the path that will incur the lowest cost to that traffic. In the limit of an infinitesimally small amount of traffic for a particular router, its routing that traffic via an ISPA is optimal, as far as cost incurred by that traffic is concerned. We demonstrate though that in many cases, due to the side-effects of one router's actions on another routers performance, having routers use ISPA's is suboptimal as far as global aggregate cost is concerned, even when only used to route infinitesimally small amounts of traffic. As a particular example of this we present an instance of Braess' paradox for ISPA'S, in which adding new links to a network decreases overall throughput. We also demonstrate that load-balancing, in which the routing decisions are made to optimize the global cost incurred by all traffic currently being routed, is suboptimal as far as global cost averaged across time is concerned. This is also due to "side-effects", in this case of current routing decision on future traffic. The theory of COllective INtelligence (COIN) is concerned precisely with the issue of avoiding such deleterious side-effects. We present key concepts from that theory and use them to derive an idealized algorithm whose performance is better than that of the ISPA, even in the infinitesimal limit. We present experiments verifying this, and also showing that a machine-learning-based version of this COIN algorithm in which costs are only imprecisely estimated (a version potentially applicable in the real world) also outperforms the ISPA, despite having access to less information than does the ISPA. In particular, this COIN algorithm avoids Braess' paradox.

  13. Experiential Avoidance and Technological Addictions in Adolescents.

    PubMed

    García-Oliva, Carlos; Piqueras, José A

    2016-06-01

    Background and aims This study focuses on the use of popular information and communication technologies (ICTs) by adolescents: the Internet, mobile phones, and video games. The relationship of ICT use and experiential avoidance (EA), a construct that has emerged as underlying and transdiagnostic to a wide variety of psychological problems, including behavioral addictions, is examined. EA refers to a self-regulatory strategy involving efforts to control or escape from negative stimuli such as thoughts, feelings, or sensations that generate strong distress. This strategy, which may be adaptive in the short term, is problematic if it becomes an inflexible pattern. Thus, the aim of this study was to explore whether EA patterns were associated with addictive or problematic use of ICT in adolescents. Methods A total of 317 students of the Spanish southeast between 12 and 18 years old were recruited to complete a questionnaire that included questions about general use of each ICTs, an experiential avoidance questionnaire, a brief inventory of the Big Five personality traits, and specific questionnaires on problematic use of the Internet, mobile phones, and video games. Results Correlation analysis and linear regression showed that EA largely explained results regarding the addictive use of the Internet, mobile phones, and video games, but not in the same way. As regards gender, boys showed a more problematic use of video games than girls. Concerning personality factors, conscientiousness was related to all addictive behaviors. Discussion and conclusions We conclude that EA is an important construct that should be considered in future models that attempt to explain addictive behaviors.

  14. "Watch out!": Effects of instructed threat and avoidance on human free-operant approach-avoidance behavior.

    PubMed

    Schlund, Michael W; Treacher, Kay; Preston, Oli; Magee, Sandy K; Richman, David M; Brewer, Adam T; Cameron, Gemma; Dymond, Simon

    2017-01-01

    Approach-avoidance paradigms create a competition between appetitive and aversive contingencies and are widely used in nonhuman research on anxiety. Here, we examined how instructions about threat and avoidance impact control by competing contingencies over human approach-avoidance behavior. Additionally, Experiment 1 examined the effects of threat magnitude (money loss amount) and avoidance cost (fixed ratio requirements), whereas Experiment 2 examined the effects of threat information (available, unavailable and inaccurate) on approach-avoidance. During the task, approach responding was modeled by reinforcing responding with money on a FR schedule. By performing an observing response, participants produced an escalating "threat meter". Instructions stated that the threat meter levels displayed the current probability of losing money, when in fact loss only occurred when the level reached the maximum. Instructions also stated pressing an avoidance button lowered the threat level. Overall, instructions produced cycles of approach and avoidance responding with transitions from approach to avoidance when threat was high and transitions back to approach after avoidance reduced threat. Experiment 1 revealed increasing avoidance cost, but not threat magnitude, shifted approach-avoidance transitions to higher threat levels and increased anxiety ratings, but did not influence the frequency of approach-avoidance cycles. Experiment 2 revealed when threat level information was available or absent earnings were high, but earnings decreased when inaccurate threat information was incompatible with contingencies. Our findings build on prior nonhuman and human approach-avoidance research by highlighting how instructed threat and avoidance can impact human AA behavior and self-reported anxiety.

  15. Real-Time Collision Avoidance for Dexterous 7-DOF Arms

    NASA Technical Reports Server (NTRS)

    Bon, Bruce; Seraji, Homayoun

    1996-01-01

    A new approach to real-time collison avoidance for dexterous 7-DOF arms and supportive simulation and experimental results are presented. The collision avoidance problem is formulated and solved as a force control problem.

  16. Collision avoidance timing analysis of DSRC-based vehicles.

    PubMed

    Tang, Antony; Yip, Alice

    2010-01-01

    Dedicated short-range communication (DSRC) has been used in prototyped vehicles to test vehicle-to-vehicle communication for collision avoidance. However, there is little study on how collision avoidance software should behave to best mitigate accident collisions. In this paper, we analyse the timing of events and how they influence software-based collision avoidance strategies. We have found that the warning strategies for collision avoidance are constrained by the timing of events such as DSRC communication latency, detection range, road condition, driver reaction and deceleration rate. With these events, we define two collision avoidance timings: critical time to avoid collision and preferred time to avoid collision, and they dictate the design of software-based collision avoidance systems.

  17. [What type of avoidance for peanut allergic children?].

    PubMed

    Feuillet-Dassonval, C; Agne, P-S-A; Rancé, F; Bidat, E

    2006-09-01

    We analyzed, from the literature, the balance benefit/risk of a strict avoidance of peanut in children with peanut allergy. The benefits of a strict avoidance diet seem limited: reactions to the low doses and to the peanut oil refined are rare and most often slight. It is not proven that a strict avoidance facilitates the cure of allergy. On the other hand, strict avoidance could induce a worsening of allergy, with deterioration of quality of life, creation of food neophobia. In case of cure of allergy, it is difficult to normalize the diet after a strict avoidance. Outside of the rare sensitive patients to a very low dose of peanut, for which a strict avoidance is counseled, the report benefits risk is in favor of the prescription of adapted avoidance to the eliciting dose. For the majority of the peanut allergic children, it seems to us that the avoidance can and must be limited to the non hidden peanut.

  18. Replication of Tobamovirus RNA.

    PubMed

    Ishibashi, Kazuhiro; Ishikawa, Masayuki

    2016-08-04

    Tobacco mosaic virus and other tobamoviruses have served as models for studying the mechanisms of viral RNA replication. In tobamoviruses, genomic RNA replication occurs via several steps: (a) synthesis of viral replication proteins by translation of the genomic RNA; (b) translation-coupled binding of the replication proteins to a 5'-terminal region of the genomic RNA; (c) recruitment of the genomic RNA by replication proteins onto membranes and formation of a complex with host proteins TOM1 and ARL8; (d) synthesis of complementary (negative-strand) RNA in the complex; and (e) synthesis of progeny genomic RNA. This article reviews current knowledge on tobamovirus RNA replication, particularly regarding how the genomic RNA is specifically selected as a replication template and how the replication proteins are activated. We also focus on the roles of the replication proteins in evading or suppressing host defense systems.

  19. Extracellular RNA in aging.

    PubMed

    Dluzen, Douglas F; Noren Hooten, Nicole; Evans, Michele K

    2017-03-01

    Since the discovery of extracellular RNA (exRNA) in circulation and other bodily fluids, there has been considerable effort to catalog and assess whether exRNAs can be used as markers for health and disease. A variety of exRNA species have been identified including messenger RNA and noncoding RNA such as microRNA (miRNA), small nucleolar RNA, transfer RNA, and long noncoding RNA. Age-related changes in exRNA abundance have been observed, and it is likely that some of these transcripts play a role in aging. In this review, we summarize the current state of exRNA profiling in various body fluids and discuss age-related changes in exRNA abundance that have been identified in humans and other model organisms. miRNAs, in particular, are a major focus of current research and we will highlight and discuss the potential role that specific miRNAs might play in age-related phenotypes and disease. We will also review challenges facing this emerging field and various strategies that can be used for the validation and future use of exRNAs as markers of aging and age-related disease. WIREs RNA 2017, 8:e1385. doi: 10.1002/wrna.1385 For further resources related to this article, please visit the WIREs website.

  20. Transcription and translation in an RNA world

    PubMed Central

    Taylor, William R

    2006-01-01

    The RNA world hypothesis requires a ribozyme that was an RNA-directed RNA polymerase (ribopolymerase). If such a replicase makes a reverse complementary copy of any sequence (including itself), in a simple RNA world, there is no mechanism to prevent self-hybridization. It is proposed that this can be avoided through the synthesis of a parallel complementary copy. The logical consequences of this are pursued and developed in a computer simulation, where the behaviour of the parallel copy is compared to the conventional reverse complementary copy. It is found that the parallel copy is more efficient at higher temperatures (up to 90°C). A model for the ribopolymerase, based on the core of the large subunit (LSU) of the ribosome, is described. The geometry of a potential active site for this ribopolymerase suggests that it contained a cavity (now occupied by the aminoacyl-tRNA) and that an amino acid binding in this might have ‘poisoned’ the ribopolymerase by cross-reacting with the nucleoside-triphosphate before polymerization could occur. Based on a similarity to the active site components of the class-I tRNA synthetase enzymes, it is proposed that the amino acid could become attached to the nascent RNA transcript producing a variety of aminoacylated tRNA-like products. Using base-pairing interactions, some of these molecules might cross-link two ribopolymerases, giving rise to a precursor of the modern ribosome. A hybrid dimer, half polymerase and half proto-ribosome, could account for mRNA translocation before the advent of protein elongation factors. PMID:17008216

  1. Landslide disaster avoidance: learning from Leyte

    NASA Astrophysics Data System (ADS)

    Davies, T. R.

    2006-12-01

    On 17 February 2006 a gigantic rockslide triggered a debris avalanche that overran the barangay Guinsaugon, St. Bernard in Southern Leyte Province, Philippines, burying 154 victims, with 990 missing including 246 school children. Even with satellite imagery, GIS-based landslide susceptibility modelling and real-time meteorological and seismic data analysis, scientific prediction of every potentially fatal landslide is not possible in most parts of the world. This is particular the case in steep, unstable, densely-populated country in which heavy rain is common. So how can further events of this type be prevented from turning into disasters? A number of precursory phenomena were noted by local inhabitants at Guinsaugon: a crack around the slope that failed was noticed in May 2005; coconut trees near the northern foot of the landslide scarp began to lean increasingly in the down-slope direction in December 2005; a slope around the northern edge of the 17 February 2006 landslide scarp failed on December 17, 2005; in the 9 days prior to the rockslide, 640 mm of rain fell; 450 mm in a 3-day period. Such phenomena are commonly reported by local inhabitants before large landslides (e.g. Elm, Mayunmarca, and many others). In many cases, therefore, it is in principle possible for local people to avoid the consequences of the landslide if they know enough to act appropriately in response to the precursory phenomena. For this possibility to be realized, appropriate information must be provided to and assimilated by the local population. Useful ways of achieving this include pamphlets, video, TV and radio programs and visits from civil defence personnel. The information must be properly presented; scientific language will be ineffective. A communication pyramid, leading from government agencies to local leaders, can facilitate the rapid availability of the information to all potentially susceptible communities. If science can determine those areas not vulnerable to landslide

  2. Adaptive limit margin detection and limit avoidance

    NASA Astrophysics Data System (ADS)

    Yavrucuk, Ilkay

    This thesis concerns the development of methods, algorithms, and control laws for the development of an adaptive flight envelope protection system to be used for both manned and unmanned aircraft. The proposed method lifts the requirement for detailed a priori information of aircraft dynamics by enabling adaptation to system uncertainty. The system can be used for limits that can be either measured or related to selected measurable quantities. Specifically, an adaptive technique for predicting limit margins and calculating the corresponding allowable control or controller command margins of an aircraft is described in an effort to enable true carefree maneuvering. This new approach utilizes adaptive neural network based loops for the approximation of required aircraft dynamics. For limits that reach their maximum value in steady state, a constructed estimator model is used to predict the maneuvering quasi-steady response behavior---the so called dynamic trim---of the limit parameters and the corresponding control or command margins. Linearly Parameterized Neural Networks as well as Single Hidden Layer Neural Networks are used for on-line adaptation. The approach does not require any off-line training of the neural networks, instead all learning is achieved during flight. Lyapunov based weight update laws are derived. The method is extended for multi-channelled control limiting for aircraft subject to multiple limits, and for automatic control and command limiting for UAV's. Simulation evaluations of the method using a linear helicopter model and a nonlinear Generalized Tiltrotor Simulation (GTRSIM) model are presented. Limit avoidance methods are integrated and tested through the implementation of an artificial pilot model and an active-stick controller model for tactile cueing in the tiltrotor simulation, GTRSIM. Load factor, angle-of-attack, and torque limits are considered as examples. Similarly, the method is applied to the Georgia Tech's Yamaha R-Max (GTMax

  3. 34 CFR 75.611 - Avoidance of flood hazards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false Avoidance of flood hazards. 75.611 Section 75.611... by a Grantee? Construction § 75.611 Avoidance of flood hazards. In planning the construction, a...) Evaluate flood hazards in connection with the construction; and (b) As far as practicable, avoid...

  4. 34 CFR 75.611 - Avoidance of flood hazards.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 1 2013-07-01 2013-07-01 false Avoidance of flood hazards. 75.611 Section 75.611... by a Grantee? Construction § 75.611 Avoidance of flood hazards. In planning the construction, a...) Evaluate flood hazards in connection with the construction; and (b) As far as practicable, avoid...

  5. 34 CFR 75.611 - Avoidance of flood hazards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Avoidance of flood hazards. 75.611 Section 75.611... by a Grantee? Construction § 75.611 Avoidance of flood hazards. In planning the construction, a...) Evaluate flood hazards in connection with the construction; and (b) As far as practicable, avoid...

  6. 34 CFR 75.611 - Avoidance of flood hazards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 1 2014-07-01 2014-07-01 false Avoidance of flood hazards. 75.611 Section 75.611... by a Grantee? Construction § 75.611 Avoidance of flood hazards. In planning the construction, a...) Evaluate flood hazards in connection with the construction; and (b) As far as practicable, avoid...

  7. 34 CFR 75.611 - Avoidance of flood hazards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 1 2012-07-01 2012-07-01 false Avoidance of flood hazards. 75.611 Section 75.611... by a Grantee? Construction § 75.611 Avoidance of flood hazards. In planning the construction, a...) Evaluate flood hazards in connection with the construction; and (b) As far as practicable, avoid...

  8. 14 CFR 417.231 - Collision avoidance analysis.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH SAFETY Flight Safety Analysis § 417.231 Collision avoidance analysis. (a) General. A flight safety analysis must include a collision avoidance analysis that... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Collision avoidance analysis....

  9. 14 CFR 417.231 - Collision avoidance analysis.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH SAFETY Flight Safety Analysis § 417.231 Collision avoidance analysis. (a) General. A flight safety analysis must include a collision avoidance analysis that... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Collision avoidance analysis....

  10. 14 CFR 417.231 - Collision avoidance analysis.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH SAFETY Flight Safety Analysis § 417.231 Collision avoidance analysis. (a) General. A flight safety analysis must include a collision avoidance analysis that... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Collision avoidance analysis....

  11. 14 CFR 417.231 - Collision avoidance analysis.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH SAFETY Flight Safety Analysis § 417.231 Collision avoidance analysis. (a) General. A flight safety analysis must include a collision avoidance analysis that... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Collision avoidance analysis....

  12. 14 CFR 417.231 - Collision avoidance analysis.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., DEPARTMENT OF TRANSPORTATION LICENSING LAUNCH SAFETY Flight Safety Analysis § 417.231 Collision avoidance analysis. (a) General. A flight safety analysis must include a collision avoidance analysis that... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Collision avoidance analysis....

  13. A Contemporary Behavior Analysis of Anxiety and Avoidance

    ERIC Educational Resources Information Center

    Dymond, Simon; Roche, Bryan

    2009-01-01

    Despite the central status of avoidance in explaining the etiology and maintenance of anxiety disorders, surprisingly little behavioral research has been conducted on human avoidance. In the present paper, first we provide a brief review of the empirical literature on avoidance. Next, we describe the implications of research on derived relational…

  14. 47 CFR 51.609 - Determination of avoided retail costs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Determination of avoided retail costs. 51.609... (CONTINUED) INTERCONNECTION Resale § 51.609 Determination of avoided retail costs. (a) Except as provided in § 51.611, the amount of avoided retail costs shall be determined on the basis of a cost study...

  15. 50 CFR 600.510 - Gear avoidance and disposal.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Gear avoidance and disposal. 600.510... Gear avoidance and disposal. (a) Vessel and gear avoidance. (1) FFV's arriving on fishing grounds where fishing vessels are already fishing or have set their gear for that purpose must ascertain the...

  16. 50 CFR 600.510 - Gear avoidance and disposal.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Gear avoidance and disposal. 600.510... Gear avoidance and disposal. (a) Vessel and gear avoidance. (1) FFV's arriving on fishing grounds where fishing vessels are already fishing or have set their gear for that purpose must ascertain the...

  17. 50 CFR 600.510 - Gear avoidance and disposal.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Gear avoidance and disposal. 600.510... Gear avoidance and disposal. (a) Vessel and gear avoidance. (1) FFV's arriving on fishing grounds where fishing vessels are already fishing or have set their gear for that purpose must ascertain the...

  18. 14 CFR 129.18 - Collision avoidance system.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Collision avoidance system. 129.18 Section... § 129.18 Collision avoidance system. Effective January 1, 2005, any airplane you, as a foreign air...) A collision avoidance system equivalent to TSO C-119b (version 7.0), or a later version, capable...

  19. 14 CFR 129.18 - Collision avoidance system.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Collision avoidance system. 129.18 Section... § 129.18 Collision avoidance system. Effective January 1, 2005, any airplane you, as a foreign air...) A collision avoidance system equivalent to TSO C-119b (version 7.0), or a later version, capable...

  20. 14 CFR 121.356 - Collision avoidance system.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Collision avoidance system. 121.356 Section... Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part must be equipped and operated according to the following table: Collision Avoidance Systems If you operate...

  1. 14 CFR 121.356 - Collision avoidance system.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Collision avoidance system. 121.356 Section... Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part must be equipped and operated according to the following table: Collision Avoidance Systems If you operate...

  2. 14 CFR 129.18 - Collision avoidance system.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Collision avoidance system. 129.18 Section... § 129.18 Collision avoidance system. Effective January 1, 2005, any airplane you, as a foreign air...) A collision avoidance system equivalent to TSO C-119b (version 7.0), or a later version, capable...

  3. 14 CFR 121.356 - Collision avoidance system.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Collision avoidance system. 121.356 Section... Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part must be equipped and operated according to the following table: Collision Avoidance Systems If you operate...

  4. 14 CFR 129.18 - Collision avoidance system.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Collision avoidance system. 129.18 Section... § 129.18 Collision avoidance system. Effective January 1, 2005, any airplane you, as a foreign air...) A collision avoidance system equivalent to TSO C-119b (version 7.0), or a later version, capable...

  5. 14 CFR 121.356 - Collision avoidance system.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Collision avoidance system. 121.356 Section... Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part must be equipped and operated according to the following table: Collision Avoidance Systems If you operate...

  6. Neuroimaging the temporal dynamics of human avoidance to sustained threat.

    PubMed

    Schlund, Michael W; Hudgins, Caleb D; Magee, Sandy; Dymond, Simon

    2013-11-15

    Many forms of human psychopathology are characterized by sustained negative emotional responses to threat and chronic behavioral avoidance, implicating avoidance as a potential transdiagnostic factor. Evidence from both nonhuman neurophysiological and human neuroimaging studies suggests a distributed frontal-limbic-striatal brain network supports avoidance. However, our understanding of the temporal dynamics of the network to sustained threat that prompts sustained avoidance is limited. To address this issue, 17 adults were given extensive training on a modified free-operant avoidance task in which button pressing avoided money loss during a sustained threat period. Subsequently, subjects underwent functional magnetic resonance imaging while completing the avoidance task. In our regions of interest, we observed phasic, rather than sustained, activation during sustained threat in dorsolateral and inferior frontal regions, anterior and dorsal cingulate, ventral striatum and regions associated with emotion, including the amygdala, insula, substantia nigra and bed nucleus of the stria terminalis complex. Moreover, trait levels of experiential avoidance were negatively correlated with insula, hippocampal and amygdala activation. These findings suggest knowledge that one can consistently avoid aversive outcomes is not associated with decreased threat-related responses and that individuals with greater experiential avoidance exhibit reduced reactivity to initial threat. Implications for understanding brain mechanisms supporting human avoidance and psychological theories of avoidance are discussed.

  7. Antibodies against RNA hydrolyze RNA and DNA.

    PubMed

    Krasnorutskii, Michael A; Buneva, Valentina N; Nevinsky, Georgy A

    2008-01-01

    Immunization of animals with DNA leads to the production of anti-DNA antibodies (Abs) demonstrating both DNase and RNase activities. It is currently not known whether anti-RNA Abs can possess nuclease activities. In an attempt to address this question, we have shown that immunization of three rabbits with complex of RNA with methylated BSA (mBSA) stimulates production of IgGs with RNase and DNase activities belonging to IgGs, while polyclonal Abs from three non-immunized rabbits and three animals immunized with mBSA are catalytically inactive. Affinity chromatography of IgGs from the sera of autoimmune (AI) patients on DNA-cellulose usually demonstrates a number of fractions, all of which effectively hydrolyze both DNA and RNA, while rabbit catalytic IgGs were separated into Ab subfractions, some of which demonstrated only DNase activity, while others hydrolyzed RNA faster than DNA. The enzymic properties of the RNase and DNase IgGs from rabbits immunized with RNA distinguish them from all known canonical RNases and DNases and DNA- and RNA-hydrolyzing abzymes (Abzs) from patients with different AI diseases. In contrast to RNases and AI RNA-hydrolyzing Abs, rabbit RNase IgGs catalyze only the first step of the hydrolysis reaction but cannot hydrolyze the formed terminal 2',3'-cyclophosphate. The data indicate that Abzs of AI patients hydrolyzing nucleic acids in part may be Abs against RNA and its complexes with proteins.

  8. RNA-Catalyzed RNA Ligation on an External RNA Template

    NASA Technical Reports Server (NTRS)

    McGinness, Kathleen E.; Joyce, Gerald F.

    2002-01-01

    Variants of the hc ligase ribozyme, which catalyzes ligation of the 3' end of an RNA substrate to the 5' end of the ribozyme, were utilized to evolve a ribozyme that catalyzes ligation reactions on an external RNA template. The evolved ribozyme catalyzes the joining of an oligonucleotide 3'-hydroxyl to the 5'-triphosphate of an RNA hairpin molecule. The ribozyme can also utilize various substrate sequences, demonstrating a largely sequence-independent mechanism for substrate recognition. The ribozyme also carries out the ligation of two oligonucleotides that are bound at adjacent positions on a complementary template. Finally, it catalyzes addition of mononucleoside '5-triphosphates onto the '3 end of an oligonucleotide primer in a template-dependent manner. The development of ribozymes that catalyze polymerase-type reactions contributes to the notion that an RNA world could have existed during the early history of life on Earth.

  9. The effects of environmental enrichment and age-related differences on inhibitory avoidance in zebrafish (Danio rerio Hamilton).

    PubMed

    Manuel, Remy; Gorissen, Marnix; Stokkermans, Mitchel; Zethof, Jan; Ebbesson, Lars O E; van de Vis, Hans; Flik, Gert; van den Bos, Ruud

    2015-04-01

    The inhibitory avoidance paradigm allows the study of mechanisms underlying learning and memory formation in zebrafish (Danio rerio Hamilton). For zebrafish, the physiology and behavior associated with this paradigm are as yet poorly understood. We therefore assessed the effects of environmental enrichment and fish age on inhibitory avoidance learning. Fish raised in an environmentally enriched tank showed decreased anxiety-like behavior and increased exploration. Enrichment greatly reduced inhibitory avoidance in 6-month (6M)- and 12-month (12 M)-old fish. Following inhibitory avoidance, telencephalic mRNA levels of proliferating cell nuclear antigen (pcna), neurogenic differentiation (neurod), cocaine- and amphetamine-regulated transcript 4 (cart4), and cannabinoid receptor 1 (cnr1) were lower in enriched-housed fish, while the ratios of mineralocorticoid receptor (nr3c2)/glucocorticoid receptor α [nr3c1(α)] and glucocorticoid receptor β [nr3c1(β)]/glucocorticoid receptor α [nr3c1(α)] were higher. This was observed for 6M-old fish only, not for 24-month (24 M) old fish. Instead, 24 M-old fish showed delayed inhibitory avoidance, no effects of enrichment, and reduced expression of neuroplasticity genes. Overall, our data show strong differences in inhibitory avoidance behavior between zebrafish of different ages and a clear reduction in avoidance behavior following housing under environmental enrichment.

  10. An expert system for wind shear avoidance

    NASA Technical Reports Server (NTRS)

    Stengel, Robert F.; Stratton, D. Alexander

    1990-01-01

    A study of intelligent guidance and control concepts for protecting against the adverse effects of wind shear during aircraft takeoffs and landings is being conducted, with current emphasis on developing an expert system for wind shear avoidance. Principal objectives are to develop methods for assessing the likelihood of wind shear encounter (based on real-time information in the cockpit), for deciding what flight path to pursue (e.g., takeoff abort, landing go-around, or normal climbout or glide slope), and for using the aircraft's full potential for combating wind shear. This study requires the definition of both deterministic and statistical techniques for fusing internal and external information , for making go/no-go decisions, and for generating commands to the manually controlled flight. The program has begun with the development of the WindShear Safety Advisor, an expert system for pilot aiding that is based on the FAA Windshear Training Aid; a two-volume manual that presents an overview , pilot guide, training program, and substantiating data provides guidelines for this initial development. The WindShear Safety Advisor expert system currently contains over 200 rules and is coded in the LISP programming language.

  11. Avoided valence transition in a plutonium superconductor

    PubMed Central

    Ramshaw, B. J.; Shekhter, Arkady; McDonald, Ross D.; Betts, Jon B.; Mitchell, J. N.; Tobash, P. H.; Mielke, C. H.; Bauer, E. D.; Migliori, Albert

    2015-01-01

    The d and f electrons in correlated metals are often neither fully localized around their host nuclei nor fully itinerant. This localized/itinerant duality underlies the correlated electronic states of the high-Tc cuprate superconductors and the heavy-fermion intermetallics and is nowhere more apparent than in the 5f valence electrons of plutonium. Here, we report the full set of symmetry-resolved elastic moduli of PuCoGa5—the highest Tc superconductor of the heavy fermions (Tc = 18.5 K)—and find that the bulk modulus softens anomalously over a wide range in temperature above Tc. The elastic symmetry channel in which this softening occurs is characteristic of a valence instability—therefore, we identify the elastic softening with fluctuations of the plutonium 5f mixed-valence state. These valence fluctuations disappear when the superconducting gap opens at Tc, suggesting that electrons near the Fermi surface play an essential role in the mixed-valence physics of this system and that PuCoGa5 avoids a valence transition by entering the superconducting state. The lack of magnetism in PuCoGa5 has made it difficult to reconcile with most other heavy-fermion superconductors, where superconductivity is generally believed to be mediated by magnetic fluctuations. Our observations suggest that valence fluctuations play a critical role in the unusually high Tc of PuCoGa5. PMID:25737548

  12. Avoided valence transition in a plutonium superconductor.

    PubMed

    Ramshaw, B J; Shekhter, Arkady; McDonald, Ross D; Betts, Jon B; Mitchell, J N; Tobash, P H; Mielke, C H; Bauer, E D; Migliori, Albert

    2015-03-17

    The d and f electrons in correlated metals are often neither fully localized around their host nuclei nor fully itinerant. This localized/itinerant duality underlies the correlated electronic states of the high-Tc cuprate superconductors and the heavy-fermion intermetallics and is nowhere more apparent than in the 5f valence electrons of plutonium. Here, we report the full set of symmetry-resolved elastic moduli of PuCoGa5--the highest Tc superconductor of the heavy fermions (Tc = 18.5 K)--and find that the bulk modulus softens anomalously over a wide range in temperature above Tc. The elastic symmetry channel in which this softening occurs is characteristic of a valence instability--therefore, we identify the elastic softening with fluctuations of the plutonium 5f mixed-valence state. These valence fluctuations disappear when the superconducting gap opens at Tc, suggesting that electrons near the Fermi surface play an essential role in the mixed-valence physics of this system and that PuCoGa5 avoids a valence transition by entering the superconducting state. The lack of magnetism in PuCoGa5 has made it difficult to reconcile with most other heavy-fermion superconductors, where superconductivity is generally believed to be mediated by magnetic fluctuations. Our observations suggest that valence fluctuations play a critical role in the unusually high Tc of PuCoGa5.

  13. Whole arm obstacle avoidance for teleoperated robots

    SciTech Connect

    Feddema, J.T.; Novak, J.L.

    1993-10-01

    This paper describes a collision avoidance system using Whole Arm Proximity (WHAP) sensors on a PUMA 560 robot arm. The capacitance-based sensors generate electric fields which can completely encompass the robot arm and detect obstacles as they approach from any direction. The directional obstacle information gathered by the WHAP sensors together with the sensor geometry and robot configuration is used to scale the commanded joint velocities of the robot. A linearized relationship between the WHAP sensor reading and the distance from the obstacle allows direct transformation of perturbations in VHAP readings to perturbations in joint velocities. The VHAP reading is used to directly reduce the component of the command input velocity along the normal axis of the sensor, allowing graceful reductions in speed as the arm approaches the obstacle. By scaling only the component of the velocity vector in the,direction of the nearest obstacles, the control system restricts motion in the direction of obstacles while permitting unconstrained motion in other directions.

  14. Approach and avoidance motivation in eating disorders.

    PubMed

    Harrison, Amy; Treasure, Janet; Smillie, Luke D

    2011-08-15

    It has been proposed that approach and avoidance processes may be critically involved in the development and maintenance of eating disorders (EDs), including anorexia nervosa (AN) and bulimia nervosa (BN). The Behavioural Inhibition System and Behavioural Activation System Scales (BIS/BAS) and Appetitive Motivation Scale (AMS) questionnaires were administered to 286 participants: 91 healthy controls (HCs), 121 participants with a current ED, either AN (restrictive and binge purge subtypes), or BN and 74 participants recovered from an ED. Individuals with EDs had higher levels of sensitivity to punishment and lower levels of reward reactivity than controls. Individuals in recovery from an ED scored the same as those in the acute group, with the exception of BAS fun seeking, for which they scored significantly higher than those with restricting AN. Discriminant analysis revealed that HCs were maximally separated from those in the acute and recovered ED groups along a dimension reflecting high punishment sensitivity and low reward sensitivity. Classification analysis demonstrated that ED and HC group membership was predicted from reward and punishment sensitivity measures; however recovered participants tended to be misclassified as ED. This study suggests high punishment sensitivity and low reward reactivity/sensitivity might form a personality cluster associated with the risk of developing an ED.

  15. Cooperative organic mine avoidance path planning

    NASA Astrophysics Data System (ADS)

    McCubbin, Christopher B.; Piatko, Christine D.; Peterson, Adam V.; Donnald, Creighton R.; Cohen, David

    2005-06-01

    The JHU/APL Path Planning team has developed path planning techniques to look for paths that balance the utility and risk associated with different routes through a minefield. Extending on previous years' efforts, we investigated real-world Naval mine avoidance requirements and developed a tactical decision aid (TDA) that satisfies those requirements. APL has developed new mine path planning techniques using graph based and genetic algorithms which quickly produce near-minimum risk paths for complicated fitness functions incorporating risk, path length, ship kinematics, and naval doctrine. The TDA user interface, a Java Swing application that obtains data via Corba interfaces to path planning databases, allows the operator to explore a fusion of historic and in situ mine field data, control the path planner, and display the planning results. To provide a context for the minefield data, the user interface also renders data from the Digital Nautical Chart database, a database created by the National Geospatial-Intelligence Agency containing charts of the world's ports and coastal regions. This TDA has been developed in conjunction with the COMID (Cooperative Organic Mine Defense) system. This paper presents a description of the algorithms, architecture, and application produced.

  16. The amygdala: securing pleasure and avoiding pain

    PubMed Central

    Fernando, Anushka B. P.; Murray, Jennifer E.; Milton, Amy L.

    2013-01-01

    The amygdala has traditionally been associated with fear, mediating the impact of negative emotions on memory. However, this view does not fully encapsulate the function of the amygdala, nor the impact that processing in this structure has on the motivational limbic corticostriatal circuitry of which it is an important structure. Here we discuss the interactions between different amygdala nuclei with cortical and striatal regions involved in motivation; interconnections and parallel circuitries that have become increasingly understood in recent years. We review the evidence that the amygdala stores memories that allow initially motivationally neutral stimuli to become associated through pavlovian conditioning with motivationally relevant outcomes which, importantly, can be either appetitive (e.g. food) or aversive (e.g. electric shock). We also consider how different psychological processes supported by the amygdala such as conditioned reinforcement and punishment, conditioned motivation and suppression, and conditioned approach and avoidance behavior, are not only psychologically but also neurobiologically dissociable, being mediated by distinct yet overlapping neural circuits within the limbic corticostriatal circuitry. Clearly the role of the amygdala goes beyond encoding aversive stimuli to also encode the appetitive, requiring an appreciation of the amygdala's mediation of both appetitive and fearful behavior through diverse psychological processes. PMID:24367307

  17. Avoiding conflicts over Africa's water resources.

    PubMed

    Ashton, Peter J

    2002-05-01

    Some 85% of Africa's water resources are comprised of large river basins that are shared between several countries. High rates of population growth accompanied by continued increases in the demand for water have resulted in several countries passing the point where the scarcity of water supplies effectively limits further development. Present population trends and patterns of water use suggest that more African countries will exceed the limits of their economically usable, land-based water resources before 2025. Normally, water allocation and distribution priorities within a country are aligned with national development objectives. While this may achieve national "water security" objectives, greater emphasis needs to be placed on regional efforts to ensure that the available water resources are used to derive sustainable long-term benefits for the peoples of Africa as a whole. Ideally, each country's water-resource management strategy needs to be aligned with that of its neighbors if peace and prosperity are to be maintained and conflict is to be avoided in the region.

  18. SAMPEX science pointing with velocity avoidance

    NASA Astrophysics Data System (ADS)

    Frakes, Joseph P.; Henretty, Debra A.; Flatley, Thomas W.; Markley, F. L.; San, Josephine K.; Lightsey, E. G.

    The Solar, Anomalous, and Magnetospheric Particle Explorer (SAMPEX) science pointing mode is presented with the additional constraint of velocity avoidance. This constraint has been added in light of the orbital debris and micrometeoroid fluxes that have been revealed by the Long Duration Exposure Facility (LDEF) recovered in January 1990. These fluxes are 50-100 times higher than the flux tables that were used in the September 1988 proposal to NASA for the SAMPEX mission. The SAMPEX Heavey Ion Large Telescope (HILT) sensor includes a flow-through isobutane proportional counter that is susceptible to penetration by orbital debris and micrometeoroids. Thus, keeping the HILT sensor pointed away from the velocity vector, the direction of maximum flux, will compensate for the higher than expected fluxes. Using an orbital debris model and a micrometeoroid model developed at the Johnson Space Center (JSC), and a SAMPEX dynamic simulator developed by the Guidance and Control Branch at the Goddard Space Flight Center (GSFC), an 'optimal' minimum ram angle (the angle between the HILT boresight and the velocity vector) of 90 degrees has been determined. It is optimal in the sense of minimizing the science pointing performance degradation while providing approximately an 89 percent chance of survival for the HILT sensor over a three year period.

  19. Nanospray 'taxation' and how to avoid it.

    PubMed

    Chernushevich, I V; Bahr, U; Karas, M

    2004-01-01

    In mass spectrometric analysis with nanospray ionization, some analytes were found to appear in spectra with a delay of tens of minutes, while a few others could not be detected at all. The effect was found to be related to cation-exchange chromatography with negative charge on the glass surface, and with the most affected peptide or protein ions having strong localization of positive charge in blocks of two or more adjacent basic amino acid residues (e.g. melittin). The 'affinity' to the glass surface was studied with a peptide mixture and bovine serum albumin (BSA) tryptic digest solutions at sub-micromolar concentration. About 20% fewer tryptic peptides could be identified from spectra recorded with a glass nanospray capillary compared to those acquired with either conventional 1 microL/min electrospray or a quartz nanospray capillary. Protein identification studies are not likely to be seriously affected by this loss, but other protein applications, such as investigations of mutations or post-translational modifications, may suffer due to reduced sequence coverage. Ways to avoid losses of useful ions are discussed.

  20. Laying hens learn to avoid feathers.

    PubMed

    Harlander-Matauschek, A; Wassermann, F; Zentek, J; Bessei, W

    2008-09-01

    Previous work demonstrated an association between feather pecking and feather eating in laying hens. This raised the question if digestive feedback affects feather eating or feather pecking in laying hens. We hypothesized that feathers enriched with sugar form a positive feedback and feathers enriched with quinine sulfate form a negative feedback. Forty-eight laying hens were kept in individual cages and fed a pelleted diet ad libitum. Twenty-four birds were offered feathers on a daily basis; 12 of these birds were offered feathers soaked in 4% quinine sulfate solution (Q), and the other 12 were offered feathers soaked in 4% sucrose solution (S). The other 24 birds were kept as a control (C) without access to feathers. After a 10-d feather feeding period, 3 groups of 4 S and 4 C birds each and 3 groups of 4 Q and 4 C birds each were assembled. Feather-pecking behavior was recorded over a period of 8 d. The number of Q feathers eaten was significantly lower than the number of S feathers. Birds that were offered Q feathers in the feather feeding phase showed significantly less severe feather pecking than S and C birds. The results clearly show that Q as an unpalatable substance was the signal the animal used to avoid damaging the feather cover in laying hens.

  1. Aerial vehicles collision avoidance using monocular vision

    NASA Astrophysics Data System (ADS)

    Balashov, Oleg; Muraviev, Vadim; Strotov, Valery

    2016-10-01

    In this paper image-based collision avoidance algorithm that provides detection of nearby aircraft and distance estimation is presented. The approach requires a vision system with a single moving camera and additional information about carrier's speed and orientation from onboard sensors. The main idea is to create a multi-step approach based on a preliminary detection, regions of interest (ROI) selection, contour segmentation, object matching and localization. The proposed algorithm is able to detect small targets but unlike many other approaches is designed to work with large-scale objects as well. To localize aerial vehicle position the system of equations relating object coordinates in space and observed image is solved. The system solution gives the current position and speed of the detected object in space. Using this information distance and time to collision can be estimated. Experimental research on real video sequences and modeled data is performed. Video database contained different types of aerial vehicles: aircrafts, helicopters, and UAVs. The presented algorithm is able to detect aerial vehicles from several kilometers under regular daylight conditions.

  2. Allergen avoidance approaches in food allergy management.

    PubMed

    Koletzko, Sibylle; Koletzko, Berthold

    2009-01-01

    Dietary elimination of causative food ingredients, usually food proteins, is the basis of treating food hypersensitivity. Proper diagnostic assessment is essential to avoid burdening children with unnecessary dietary restrictions with potential adverse effects. Diagnosis requires a detailed history, allergen elimination, and re-challenge with suspected foods. Complete elimination of causative food components depends on professional counseling and training of the patient and family, and transparent labeling of food products. Elimination diets carry the risk of inducing insufficient supplies of critical nutrients with adverse effects on health and wellbeing, particularly in children with exclusion of foods that provide a major part of dietary supply and patients with multiple food allergies. Infants and young children with cow's milk allergy, who have not been fully breastfed, require milk substitutes based on extensively hydrolyzed protein or amino acids. Elimination diets must be supervised and monitored to a similar degree as drug treatments, and the need for continued dietary elimination should be reviewed on a regular basis and re-challenges considered.

  3. Velocity of chloroplast avoidance movement is fluence rate dependent.

    PubMed

    Kagawa, Takatoshi; Wada, Masamitsu

    2004-06-01

    In Arabidopsis leaves, chloroplast movement is fluence rate dependent. At optimal, lower light fluences, chloroplasts accumulate at the cell surface to maximize photosynthetic potential. Under high fluence rates, chloroplasts avoid incident light to escape photodamage. In this paper, we examine the phenomenon of chloroplast avoidance movement in greater detail and demonstrate a proportional relationship between fluence rate and the velocity of chloroplast avoidance. In addition we show that the amount of light-activated phototropin2, the photoreceptor for the avoidance response, likely plays a role in this phenomenon, as heterozygous mutant plants show a reduced avoidance velocity compared to that of homozygous wild type plants.

  4. Infection-avoidance behaviour in humans and other animals.

    PubMed

    Curtis, Valerie A

    2014-10-01

    Compared with living free, the parasitic way of life has many attractions. Parasites create problems for all animals. Potential hosts can respond by learning to live with parasites (tolerance), actively fighting them (resistance), or they can avoid becoming infected in the first place (avoidance). I propose here a new classification of avoidance behaviour according to the epidemiology of infection risk, where animals must avoid (i) conspecifics, (ii) parasites and their vectors, (iii) parasite-rich environments, and (iv) niche infestation. I further explore how the disgust adaptive system, which coordinates avoidance behaviour, may form a continuum with the immune system through the sharing of signalling pathways, sites of action, and evolutionary history.

  5. Faecal avoidance and selective foraging: do wild mice have the luxury to avoid faeces?

    PubMed

    Walsh, Patrick T; McCreless, Erin; Pedersen, Amy B

    2013-09-01

    Host-parasite interactions are a key determinant of the population dynamics of wild animals, and behaviours that reduce parasite transmission and infection may be important for improving host fitness. While antiparasite behaviours have been demonstrated in laboratory animals and domesticated ungulates, whether these behaviours operate in the wild is poorly understood. Therefore, examining antiparasite behaviours in natural populations is crucial for understanding their ecological significance. In this study, we examined whether two wild rodents (white-footed mice, Peromyscus leucopus, and deer mice, Peromyscus maniculatus), selectively foraged away from conspecific faeces or avoided faeces altogether, and whether faecal gastrointestinal parasite status affected their behaviour. We also tested whether wild mice, when nesting, avoided using material that had previously been used by healthy or parasite-infected conspecifics. Our results, in contrast to laboratory mouse studies, suggest that wild mice do not demonstrate faecal avoidance, selective foraging or selective use of nesting material; they preferred being near faeces and did not differentiate between faeces from parasitized and uninfected conspecifics. Behavioural avoidance to reduce parasite infection may still represent an important strategy; however, mice in our study population appeared to favour the opportunity to feed and nest over the risks of coming into contact with faecal-transmitted parasites. Furthermore, the presence of conspecific faeces may actually provide a positive cue of a good foraging or nesting location. Ultimately, balancing the trade-off of performing antiparasite behaviours to reduce infection with missing an important feeding or nesting opportunity may be very different for animals in the wild facing complex and stochastic environments.

  6. Body checking and avoidance in ethnically diverse female college students.

    PubMed

    White, Emily K; Warren, Cortney S

    2013-09-01

    Although body checking and avoidance behaviors are common in women with eating disorders, minimal research has examined the nature or correlates of these behaviors in ethnically diverse female college students without eating disorders. Self-identified European American (n=268), Asian American (n=163), Latina (n=146), and African American (n=73) women completed self-report measures of body checking and avoidance, thin-ideal internalization, eating pathology, and clinical impairment. Results indicated that European and Asian American women reported significantly more body checking and avoidance than African American and Latina women. Generally, correlates of body checking and avoidance were consistent across ethnic groups: Regression analyses indicated that type of ethnicity predicted body checking and avoidance; and ethnicity, body checking, and body avoidance predicted eating pathology and clinical impairment. These associations suggest that body checking and avoidance are not benign behaviors in diverse nonclinical women.

  7. AVOID BECOMING A VICTIM OF COUNTERFEIT ITEMS

    SciTech Connect

    WARRINER RD

    2011-07-13

    In today's globalized economy, we cannot live without imported products. Most people do not realize how thin the safety net of regulation and inspection really is. Less than three percent of imported products receive any form of government inspection prior to sale. Avoid flea markets, street vendors and deep discount stores. The sellers of counterfeit wares know where to market their products. They look for individuals who are hungry for a brand name item but do not want to pay a brand name price for it. The internet provides anonymity to the sellers of counterfeit products. Unlike Europe, U.S. law does not hold internet-marketing organizations, responsible for the quality of the products sold on their websites. These organizations will remove an individual vendor when a sufficient number of complaints are lodged, but they will not take responsibility for the counterfeit products you may have purchased. EBay has a number of counterfeit product guides to help you avoid being a victim of the sellers of these products. Ten percent of all medications taken worldwide are counterfeit. If you do buy medications on-line, be sure that the National Association of Boards of Pharmacy Verified Internet Pharmacy Practice Sites (VIPPS) recommends the pharmacy you choose to use. Inspect all medication purchases and report any change in color, shape, imprinting or odor to your pharmacist. If you take generic medications these attributes may change from one manufacturer to another. Your pharmacist should inform you of any changes when you refill your prescription. If they do not, get clarification prior to taking the medication. Please note that the Federal Drug Administration (FDA) does not regulate supplements. The FDA only steps in when a specific supplement proves to cause physical harm or contains a regulated ingredient. Due to counterfeiting, Underwriters Laboratories (UL) changed their label design three times since 1996. The new gold label should be attached to the cord or

  8. Decoding the RNA structurome

    PubMed Central

    Lu, Zhipeng; Chang, Howard Y

    2016-01-01

    Structures of RNA molecules are essential for their architectural, regulatory, and catalytic functions. Recent advances in high throughput sequencing enabled the development of methods for probing RNA structures on a transcriptome-wide scale – termed the RNA structurome. Here we review the state-of-the-art technologies for probing the RNA structurome, and highlight insights gained from these studies. We also point out the limits of current methods and discuss potential directions for future improvements. PMID:26923056

  9. Avoiding unfavorable results in postburn contracture hand

    PubMed Central

    Bhattacharya, Sameek

    2013-01-01

    Deformities of the hands are a fairly common sequel of burn especially in the developing world. This is because of high incidence of burns, limited access to standard treatment and rehabilitation. The best outcome of a burnt hand is when deformities are prevented from developing. A good functional result is possible when due consideration is paid to hands during resuscitation, excisional surgery, reconstructive surgery and physiotherapy. The post-burns deformities of hand develop due direct thermal damage or secondary to intrinsic minus position due to oedema or vascular insufficiency. During the acute phase the concerns are, maintenance circulation minimize oedema prevent unphysiological positioning and wound closure with autogenous tissue as soon as possible. The rehabilitation program during the acute phase starts from day one and goes on till the hand has healed and has regained full range of motion. Full blown hand contractures are challenging to correct and become more difficult as time passes. Long-standing cases often land up with attenuation of extensor apparatus leading to swan neck and boutonniere deformity, muscle shortening and bony ankylosis. The major and most common pitfall after contracture release is relapse. The treatment protocol of contracture is solely directed towards countering this tendency. This article aims to guide a surgeon in obtaining optimal hand function and avoid pit falls at different stages of management of hand burns. The reasons of an unfavourable outcome of a burnt hand are possible lack of optimal care in the acute phase, while planning and performing reconstructive procedure and during aftercare and rehabilitation. PMID:24501479

  10. Collision avoidance in computer optimized treatment planning.

    PubMed

    Humm, J L

    1994-07-01

    Of major concern in fully automated computerized treatment delivery is the possibility of gantry/couch or gantry/patient collisions. In this work, software has been developed to detect collisions between gantry and couch or patient for both transaxial and noncoplanar treatment fields during the treatment planning process. The code uses the gantry angles, turntable angles, and position of the couch surface relative to the isocenter supplied by the planner for the prescribed radiation fields. In addition, the maximum patient anterior-posterior and lateral separations are entered in order to model the patient outline by a conservative cylindrical ellipse. By accessing a database containing the precise mechanical dimensions of the therapy equipment, 3D analytical geometry is used to test for collisions between gantry/patient and gantry/couch for each treatment field. When collisions are detected, the software inspects the use of an extended distance treatment, by recalculating and testing for collisions, with the couch at a greater distance from the collimator along the direction of the central axis. If a collision is avoided at extended distance, the lateral, longitudinal, and vertical motions of the couch are recorded for entry into the treatment plan, or else a warning message is printed, together with the nearest permissible collision-free gantry angle. Upon inspection, the planner can either elect to use the calculated closest permissible gantry angle or reject the plan. The software verifies that each proposed treatment field is safe, but also that the transition between fields is collision-free. This requires that the sequence of the treatment fields be ordered, preferably into a sequence which minimizes the delivery time compatible with patient safety.(ABSTRACT TRUNCATED AT 250 WORDS)

  11. Green light induces shade avoidance symptoms.

    PubMed

    Zhang, Tingting; Maruhnich, Stefanie A; Folta, Kevin M

    2011-11-01

    Light quality and quantity affect plant adaptation to changing light conditions. Certain wavelengths in the visible and near-visible spectrum are known to have discrete effects on plant growth and development, and the effects of red, far-red, blue, and ultraviolet light have been well described. In this report, an effect of green light on Arabidopsis (Arabidopsis thaliana) rosette architecture is demonstrated using a narrow-bandwidth light-emitting diode-based lighting system. When green light was added to a background of constant red and blue light, plants exhibited elongation of petioles and upward leaf reorientation, symptoms consistent with those observed in a shaded light environment. The same green light-induced phenotypes were also observed in phytochrome (phy) and cryptochrome (cry) mutant backgrounds. To explore the molecular mechanism underlying the green light-induced response, the accumulation of shade-induced transcripts was measured in response to enriched green light environments. Transcripts that have been demonstrated to increase in abundance under far-red-induced shade avoidance conditions either decrease or exhibit no change when green light is added. However, normal far-red light-associated transcript accumulation patterns are observed in cryptochrome mutants grown with supplemental green light, indicating that the green-absorbing form of cryptochrome is the photoreceptor active in limiting the green light induction of shade-associated transcripts. These results indicate that shade symptoms can be induced by the addition of green light and that cryptochrome receptors and an unknown light sensor participate in acclimation to the enriched green environment.

  12. Geoengineering to Avoid Overshoot: An Uncertainty Analysis

    NASA Astrophysics Data System (ADS)

    Tanaka, K.

    2009-04-01

    Geoengineering (or climate engineering) using stratospheric sulfur injections (Crutzen, 2006) has been called for research in case of an urgent need for stopping global warming when other mitigation efforts were exhausted. Although there are a number of concerns over this idea (e.g. Robock, 2008), it is still useful to consider geoengineering as a possible method to limit warming caused by overshoot. Overshoot is a feature accompanied by low stabilizations scenarios aiming for a stringent target (Rao et al., 2008) in which total radiative forcing temporarily exceeds the target before reaching there. Scenarios achieving a 50% emission reduction by 2050 produces overshoot. Overshoot could cause sustained warming for decades due to the inertia of the climate system. If stratospheric sulfur injections were to be used as a "last resort" to avoid overshoot, what would be the suitable start-year and injection profile of such an intervention? Wigley (2006) examined climate response to combined mitigation/geoengineering scenarios with the intent to avert overshoot. Wigley's analysis demonstrated a basic potential of such a combined mitigation/geoengineering approach to avoid temperature overshoot - however it considered only simplistic sulfur injection profiles (all started in 2010), just one mitigation scenario, and did not examine the sensitivity of the climate response to any underlying uncertainties. This study builds upon Wigley's premise of the combined mitigation/geoengineering approach and brings associated uncertainty into the analysis. First, this study addresses the question as to how much geoengineering intervention would be needed to avoid overshoot by considering associated uncertainty? Then, would a geoengineering intervention of such a magnitude including uncertainty be permissible in considering all the other side effects? This study begins from the supposition that geoengineering could be employed to cap warming at 2.0°C since preindustrial. A few

  13. Assessment of avoidance behaviors in mouse models of muscle pain

    PubMed Central

    Pratt, Dane; Fuchs, Perry N.; Sluka, Kathleen A.

    2014-01-01

    Pain encompasses both a sensory as well as an affective dimension and these are differentially processed in the cortex. Animal models typically use reflexive behaviors to test nociceptive responses; these are thought to reflect the sensory dimension of pain. While several behavioral tests are available for examining the affective dimension of pain it is unclear if these are appropriate in animal models of muscle pain. We therefore tested the utility of existing paradigms as well as new avoidance paradigms in animal models of muscle pain in mice. Specifically we used an escape-avoidance test to noxious mechanical stimuli, a learned avoidance test to noxious mechanical stimuli, and avoidance of physical activity. We used three animal models of muscle pain: carrageenan-induced inflammation, non-inflammatory muscle pain, and exercise-enhanced pain. In the carrageenan model of inflammation mice developed escape-avoidance behaviors to mechanical stimuli, learned avoidance to mechanical stimulation and avoidance of physical activity – these models are associated with unilateral hyperalgesia. When both muscles were inflamed, escape-avoidance behaviors did not develop suggesting equivalent bilateral pain-behaviors cannot be tested with an escape-avoidance test. In the non-inflammatory muscle pain model mice did not show significant changes in escape-avoidance behaviors or learned avoidance, but did avoid physical activity. In the exercise-enhanced pain model, there were no changes in escape avoidance, learned avoidance of noxious or physical activity In conclusion, we developed several testing protocols that assess supraspinal processing of pain-behaviors in models of muscle pain and that are most sensitive in animals with unilateral hyperalgesia. PMID:23747349

  14. An alternative telomerase RNA in Arabidopsis modulates enzyme activity in response to DNA damage

    PubMed Central

    Cifuentes-Rojas, Catherine; Nelson, Andrew D.L.; Boltz, Kara A.; Kannan, Kalpana; She, Xintao; Shippen, Dorothy E.

    2012-01-01

    Telomerase replenishes telomere tracts by reiteratively copying its RNA template, TER. Unlike other model organisms, Arabidopsis thaliana harbors two divergent TER genes. However, only TER1 is required for telomere maintenance. Here we examine the function of TER2. We show that TER2 is spliced and its 3′ end is truncated in vivo to generate a third TER isoform, TER2S. TERT preferentially associates with TER2 > TER1 > TER2S. Moreover, TER2 and TER2S assemble with Ku and POT1b (protection of telomeres), forming RNP (ribonucleoprotein) complexes distinct from TER1 RNP. Plants null for TER2 display increased telomerase enzyme activity, while TER2 overexpression inhibits telomere synthesis from TER1 and leads to telomere shortening. These findings argue that TER2 negatively regulates telomerase by sequestering TERT in a nonproductive RNP complex. Introduction of DNA double-strand breaks by zeocin leads to an immediate and specific spike in TER2 and a concomitant decrease in telomerase enzyme activity. This response is not triggered by replication stress or telomere dysfunction and is abrogated in ter2 mutants. We conclude that Arabidopsis telomerase is modulated by TER2, a novel DNA damage-induced noncoding RNA that works in concert with the canonical TER to promote genome integrity. PMID:23109676

  15. miARma-Seq: a comprehensive tool for miRNA, mRNA and circRNA analysis

    PubMed Central

    Andrés-León, Eduardo; Núñez-Torres, Rocío; Rojas, Ana M.

    2016-01-01

    Large-scale RNAseq has substantially changed the transcriptomics field, as it enables an unprecedented amount of high resolution data to be acquired. However, the analysis of these data still poses a challenge to the research community. Many tools have been developed to overcome this problem, and to facilitate the study of miRNA expression profiles and those of their target genes. While a few of these enable both kinds of analysis to be performed, they also present certain limitations in terms of their requirements and/or the restrictions on data uploading. To avoid these restraints, we have developed a suite that offers the identification of miRNA, mRNA and circRNAs that can be applied to any sequenced organism. Additionally, it enables differential expression, miRNA-mRNA target prediction and/or functional analysis. The miARma-Seq pipeline is presented as a stand-alone tool that is both easy to install and flexible in terms of its use, and that brings together well-established software in a single bundle. Our suite can analyze a large number of samples due to its multithread design. By testing miARma-Seq in validated datasets, we demonstrate here the benefits that can be gained from this tool by making it readily accessible to the research community. PMID:27167008

  16. Adolescents' approach-avoidance behaviour in the context of pain.

    PubMed

    Fisher, Emma; Keogh, Edmund; Eccleston, Christopher

    2016-02-01

    Adolescents who experience pain often face competing goals and have to choose whether to approach (confront) or avoid pain. This study investigates the decisions adolescents make when their pain conflicts with a valued goal. Adolescents between the ages of 15 and 18 years (N = 170) completed questionnaires on general and pain-specific anxiety, courage, and dispositional avoidance. Adolescents were presented with 16 vignettes (8 high pain intensity, 8 low pain intensity), which described pain conflicting with a goal (eg, doing well at school, seeing friends). Adolescents rated goals for importance and reported how likely they would be to approach or avoid each pain. Adolescents were more likely to avoid and were more fearful of high pain intensity than low pain intensity vignettes. Pain anxiety predicted higher levels of avoidance for both pain intensities. General anxiety was not a significant predictor of avoidance for either pain intensity. Goal importance promoted approach of goals, but only when pain was described as intense. However, pain anxiety predicted avoidance beyond the importance of goals for high pain intensity vignettes. In addition, we compared approach-avoidance of adolescents with and without chronic pain; analyses revealed no differences in approach-avoidance behaviour. We also found that behavioural endurance was predictive of approach and dispositional avoidance predicted higher avoidance, but courage was not predictive of behaviour in this task. We adopt a motivational perspective when interpreting the findings and consider whether the fear-avoidance model should be extended to include the function of avoidance or approach in the pursuit of a desired goal.

  17. Cytoplasmic Z-RNA

    SciTech Connect

    Zarling, D.A.; Calhoun, C.J.; Hardin, C.C.; Zarling, A.H.

    1987-09-01

    Specific immunochemical probes for Z-RNA were generated and characterized to search for possible Z-RNA-like double helices in cells. Z-RNA was detected in the cytoplasm of fixed protozoan cells by immunofluorescence microscopy using these anti-Z-RNA IgCs. In contrast, autoimmune or experimentally elicited anti-DNA antibodies, specifically reactive with B-DNA or Z-DNA, stained the nuclei. Pre-or nonimmune IgGs did not bind to the cells. RNase A or T1 digestion eliminated anti-Z-RNA IgG binding to cytoplasmic determinants; however, DNase I or mung bean nuclease had no effect. Doxorubicin and ethidium bromide prevented anti-Z-RNA antibody binding; however, actinomycin D, which does not bind double-stranded RNA, did not. Anti-Z-RNA immunofluorescence was specifically blocked in competition assays by synthetic Z-RNA but not Z-DNA, A-RNA, or single-stranded RNAs. Thus, some cytoplasmic sequences in fixed cells exist in the left-handed Z-RNA conformation.

  18. Translating Ribosomes Inhibit Poliovirus Negative-Strand RNA Synthesis

    PubMed Central

    Barton, David J.; Morasco, B. Joan; Flanegan, James B.

    1999-01-01

    Poliovirus has a single-stranded RNA genome of positive polarity that serves two essential functions at the start of the viral replication cycle in infected cells. First, it is translated to synthesize viral proteins and, second, it is copied by the viral polymerase to synthesize negative-strand RNA. We investigated these two reactions by using HeLa S10 in vitro translation-RNA replication reactions. Preinitiation RNA replication complexes were isolated from these reactions and then used to measure the sequential synthesis of negative- and positive-strand RNAs in the presence of different protein synthesis inhibitors. Puromycin was found to stimulate RNA replication overall. In contrast, RNA replication was inhibited by diphtheria toxin, cycloheximide, anisomycin, and ricin A chain. Dose-response experiments showed that precisely the same concentration of a specific drug was required to inhibit protein synthesis and to either stimulate or inhibit RNA replication. This suggested that the ability of these drugs to affect RNA replication was linked to their ability to alter the normal clearance of translating ribosomes from the input viral RNA. Consistent with this idea was the finding that the protein synthesis inhibitors had no measurable effect on positive-strand synthesis in normal RNA replication complexes. In marked contrast, negative-strand synthesis was stimulated by puromycin and was inhibited by cycloheximide. Puromycin causes polypeptide chain termination and induces the dissociation of polyribosomes from mRNA. Cycloheximide and other inhibitors of polypeptide chain elongation “freeze” ribosomes on mRNA and prevent the normal clearance of ribosomes from viral RNA templates. Therefore, it appears that the poliovirus polymerase was not able to dislodge translating ribosomes from viral RNA templates and mediate the switch from translation to negative-strand synthesis. Instead, the initiation of negative-strand synthesis appears to be coordinately regulated

  19. Generalisation of fear and avoidance along a semantic continuum.

    PubMed

    Boyle, Sean; Roche, Bryan; Dymond, Simon; Hermans, Dirk

    2016-01-01

    Directly conditioned fear and avoidance readily generalises to dissimilar but conceptually related stimuli. Here, for the first time, we examined the conceptual/semantic generalisation of both fear and avoidance using real words (synonyms). Participants were first exposed to a differential fear conditioning procedure in which one word (e.g., "broth"; CS+) was followed with brief electric shock [unconditioned stimulus (US)] and another was not (e.g., "assist"; CS-). Next, an instrumental conditioning phase taught avoidance in the presence the CS+ but not the CS-. During generalisation testing, synonyms of the CS+ (e.g., "soup"; GCS+) and CS- (e.g., "help"; GCS-) were presented in the absence of shock. Conditioned fear and avoidance, measured via skin conductance responses, behavioural avoidance and US expectancy ratings, generalised to the semantically related, but not to the semantically unrelated, synonyms. Findings have implications for how natural language categories and concepts mediate the expansion of fear and avoidance repertoires in clinical contexts.

  20. Excise Tax Avoidance: The Case of State Cigarette Taxes

    PubMed Central

    DeCicca, Philip; Kenkel, Donald; Liu, Feng

    2013-01-01

    We conduct an applied welfare economics analysis of cigarette tax avoidance. We develop an extension of the standard formula for the optimal Pigouvian corrective tax to incorporate the possibility that consumers avoid the tax by making purchases in nearby lower-tax jurisdictions. To provide a key parameter for our formula, we estimate a structural endogenous switching regression model of border-crossing and cigarette prices. In illustrative calculations, we find that for many states, after taking into account tax avoidance the optimal tax is at least 20 percent smaller than the standard Pigouvian tax that simply internalizes external costs. Our empirical estimate that tax avoidance strongly responds to the price differential is the main reason for this result. We also use our results to examine the benefits of replacing avoidable state excise taxes with a harder-to-avoid federal excise tax on cigarettes. PMID:24140760

  1. Anxiety sensitivity moderates behavioral avoidance in anxious youth.

    PubMed

    Lebowitz, Eli R; Shic, Frederick; Campbell, Daniel; Basile, Krista; Silverman, Wendy K

    2015-11-01

    Individuals who are high in anxiety sensitivity (AS) are motivated to avoid sensations of anxiety. Consequently, AS is hypothesized to contribute to overall avoidance of any feared stimuli. No studies have yet examined whether fear of a stimulus is a stronger predictor of behavioral avoidance in individuals who are high in AS compared to individuals who are low in AS. We examined whether AS moderates the association between fear of spiders and behavioral avoidance of spider stimuli in 50 clinically anxious youth. Fear of spiders significantly predicted avoidance of spider stimuli in youth high in AS but not in youth low in AS. These results provide support for the role of AS in avoidant behavior and help to explain the link between AS and the anxiety disorders. The results have implications for exposure-based anxiety treatments and highlight the importance of increasing anxious patients' ability to tolerate sensations of anxiety.

  2. Enhancement of single guide RNA transcription for efficient CRISPR/Cas-based genomic engineering.

    PubMed

    Ui-Tei, Kumiko; Maruyama, Shohei; Nakano, Yuko

    2017-01-26

    Genomic engineering using clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein is a promising approach for targeting the genomic DNA of virtually any organism in a sequence-specific manner. Recent remarkable advances in CRISPR/Cas technology have made it a feasible system for use in therapeutic applications and biotechnology. In the CRISPR/Cas system, a guide RNA (gRNA), interacting with the Cas protein, recognizes a genomic region with sequence complementarity, and the double-stranded DNA at the target site is cleaved by the Cas protein. A widely used gRNA is an RNA polymerase III (pol III)-driven single gRNA (sgRNA), which is produced by artificial fusion of CRISPR RNA (crRNA) and trans-activation crRNA (tracrRNA). However, we identified a TTTT stretch, known as a termination signal of RNA pol III, in the scaffold region of the sgRNA. Here, we revealed that sgRNA carrying a TTTT stretch reduces the efficiency of sgRNA transcription due to premature transcriptional termination, and decreases the efficiency of genome editing. Unexpectedly, it was also shown that the premature terminated sgRNA may have an adverse effect of inducing RNA interference. Such disadvantageous effects were avoided by substituting one base in the TTTT stretch.

  3. A Mobile Robot Sonar System with Obstacle Avoidance.

    DTIC Science & Technology

    1994-03-01

    vehicle, robot, obstacle avoidance, sonar sensing 102 17. SECURITY CLASSWIFCATION 1S. SECURITY CLASINFICATION I& SECURITY CLASSIFICATION 20. UMITATION...WITH OBSTACLE - AVOIDANCE __ by __ Patrick Gerard Byrne March 1994 Thesis Advisor: Yutaka Kanayama Approved for public release; distribution is...SEOLY(Lae anO r Iff TDT, Master’s Thesis _____________ 4. TITLE AND SUBTITLE L FUMNING NUMBR A Mobile Robot Sonar System With Obstacle Avoidance (U

  4. Effects of optimism on creativity under approach and avoidance motivation

    PubMed Central

    Icekson, Tamar; Roskes, Marieke; Moran, Simone

    2014-01-01

    Focusing on avoiding failure or negative outcomes (avoidance motivation) can undermine creativity, due to cognitive (e.g., threat appraisals), affective (e.g., anxiety), and volitional processes (e.g., low intrinsic motivation). This can be problematic for people who are avoidance motivated by nature and in situations in which threats or potential losses are salient. Here, we review the relation between avoidance motivation and creativity, and the processes underlying this relation. We highlight the role of optimism as a potential remedy for the creativity undermining effects of avoidance motivation, due to its impact on the underlying processes. Optimism, expecting to succeed in achieving success or avoiding failure, may reduce negative effects of avoidance motivation, as it eases threat appraisals, anxiety, and disengagement—barriers playing a key role in undermining creativity. People experience these barriers more under avoidance than under approach motivation, and beneficial effects of optimism should therefore be more pronounced under avoidance than approach motivation. Moreover, due to their eagerness, approach motivated people may even be more prone to unrealistic over-optimism and its negative consequences. PMID:24616690

  5. Avoiding DWI Among Bar-room Drinkers: Strategies and Predictors

    PubMed Central

    Caudill, Barry D.; Rogers, John W.; Howard, Jan; Frissell, Kevin C.; Harding, Wayne M.

    2010-01-01

    We examined the prevalence and predictors of 11strategies to avoid driving when feeling intoxicated among 561 bar-room patrons in two medium-sized Maryland communities. Logistic regression analyses identified demographic, behavioral, and attitudinal predictors of avoidance strategies and interactions among predictors. Overall, 89% reported one or more DWI avoidance actions in the past year, and 38% reported driving intoxicated during that time. Average frequencies of avoidance behavior and intoxicated driving increased significantly as drinking level increased. However, the higher the drinking level, the smaller the ratio of avoidance actions to DWI experiences, highlighting the vulnerability of heavy drinkers who had driven intoxicated. Using a sober driver or one who allegedly drank less than the respondent were the most popular and frequent strategies, but paying for a cab, walking, and using a bus or free cab were relatively unpopular. Higher drinking levels predicted significantly higher odds of using avoidance approaches, as did intoxicated driving. Confidence in driving safely when intoxicated was positively related to drinking level and intoxicated driving, but it tended to predict lower odds of avoidance actions. Similarly, marital status, age, gender, and location influenced the odds of avoidance behaviors. Interventions should be strategically tailored to exploit or counter drinker predilections among avoidance options. PMID:22879742

  6. Host membrane proteins involved in the replication of tobamovirus RNA.

    PubMed

    Ishibashi, Kazuhiro; Miyashita, Shuhei; Katoh, Etsuko; Ishikawa, Masayuki

    2012-12-01

    Eukaryotic positive-strand RNA viruses replicate their genomes in membrane-bound replication complexes composed of viral replication proteins and negative-strand RNA templates. These replication proteins are programmed to exhibit RNA polymerase and other replication-related activities only in replication complexes to avoid inducing double-stranded RNA-mediated host defenses. Host membrane components (e.g. proteins and lipids) should play important roles in the activation of replication proteins. Two host membrane proteins are components of the replication complex and activate the replication proteins of tobamoviruses. Interaction analyses using deletion mutants constructed based on structural information suggest a conformational change in replication proteins during the formation of a protein complex with RNA 5'-capping activity.

  7. Who Avoids Cancer Information? Examining a Psychological Process Leading to Cancer Information Avoidance.

    PubMed

    Chae, Jiyoung

    2016-07-01

    Although cancer information avoidance (CIA) is detrimental to public health, predictors of CIA have not been fully investigated. Based on uncertainty management theory, this study viewed CIA as a response to uncertainty related to the distress associated with cancer information and illustrated the psychological process leading to CIA. Given the current information context, it was hypothesized that cancer information overload (CIO), accompanied by confusion and stress about cancer information, causes CIA. As trait anxiety is a strong predictor of CIO, it was also hypothesized that trait anxiety has an indirect effect on CIA through CIO. Study 1 tested this relationship in a U.S. sample (N = 384); the results showed that CIO was positively associated with CIA and that trait anxiety indirectly influenced CIA through CIO. Whereas Study 1 tested the relationship with cross-sectional data in the general cancer context, Study 2 replicated Study 1 with 3-wave longitudinal data in the context of a specific cancer (i.e., stomach cancer) in South Korea (N = 1,130 at Wave 1, 813 at Wave 2, and 582 at Wave 3). Trait anxiety at Wave 1 predicted CIO at Wave 2, which in turn increased CIA at Wave 3, suggesting that some people are inherently inclined to avoid cancer information due to their trait anxiety, which results in confusion about cancer information.

  8. Exponents of intrachain correlation for self-avoiding walks and knotted self-avoiding polygons

    NASA Astrophysics Data System (ADS)

    Uehara, Erica; Deguchi, Tetsuo

    2013-08-01

    We show numerically that critical exponents for two-point intrachain correlation of an infinite chain characterize those of finite chains in self-avoiding walk (SAW) and self-avoiding polygon (SAP) under a topological constraint. We evaluate short-distance exponents θ(i, j) through the probability distribution functions of the distance between the ith and jth vertices of N-step SAW (or SAP with a knot) for all pairs (1 ⩽ i, j ⩽ N). We construct the contour plot of θ(i, j), and express it as a function of i and j. We suggest that it has quite a simple structure. Here exponents θ(i, j) generalize des Cloizeaux’s three critical exponents for short-distance intrachain correlation of SAW, and we show the crossover among them. We also evaluate the diffusion coefficient of knotted SAP for a few knot types, which can be calculated with the probability distribution functions of the distance between two nodes.

  9. Repetitive RNA unwinding by RNA helicase A facilitates RNA annealing.

    PubMed

    Koh, Hye Ran; Xing, Li; Kleiman, Lawrence; Myong, Sua

    2014-07-01

    Helicases contribute to diverse biological processes including replication, transcription and translation. Recent reports suggest that unwinding of some helicases display repetitive activity, yet the functional role of the repetitiveness requires further investigation. Using single-molecule fluorescence assays, we elucidated a unique unwinding mechanism of RNA helicase A (RHA) that entails discrete substeps consisting of binding, activation, unwinding, stalling and reactivation stages. This multi-step process is repeated many times by a single RHA molecule without dissociation, resulting in repetitive unwinding/rewinding cycles. Our kinetic and mutational analysis indicates that the two double stand RNA binding domains at the N-terminus of RHA are responsible for such repetitive unwinding behavior in addition to providing an increased binding affinity to RNA. Further, the repetitive unwinding induces an efficient annealing of a complementary RNA by making the unwound strand more accessible. The complex and unusual mechanism displayed by RHA may help in explaining how the repetitive unwinding of helicases contributes to their biological functions.

  10. RNA Viruses Infecting Pest Insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    RNA viruses are viruses whose genetic material is ribonucleic acid (RNA). RNA viruses may be double or single-stranded based on the type of RNA they contain. Single-stranded RNA viruses can be further grouped into negative sense or positive-sense viruses according to the polarity of their RNA. Fur...

  11. Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I.

    PubMed

    Lama, Lodoe; Ryan, Kevin

    2016-01-01

    Many high-throughput small RNA next-generation sequencing protocols use 5' preadenylylated DNA oligonucleotide adapters during cDNA library preparation. Preadenylylation of the DNA adapter's 5' end frees from ATP-dependence the ligation of the adapter to RNA collections, thereby avoiding ATP-dependent side reactions. However, preadenylylation of the DNA adapters can be costly and difficult. The currently available method for chemical adenylylation of DNA adapters is inefficient and uses techniques not typically practiced in laboratories profiling cellular RNA expression. An alternative enzymatic method using a commercial RNA ligase was recently introduced, but this enzyme works best as a stoichiometric adenylylating reagent rather than a catalyst and can therefore prove costly when several variant adapters are needed or during scale-up or high-throughput adenylylation procedures. Here, we describe a simple, scalable, and highly efficient method for the 5' adenylylation of DNA oligonucleotides using the thermostable RNA ligase 1 from bacteriophage TS2126. Adapters with 3' blocking groups are adenylylated at >95% yield at catalytic enzyme-to-adapter ratios and need not be gel purified before ligation to RNA acceptors. Experimental conditions are also reported that enable DNA adapters with free 3' ends to be 5' adenylylated at >90% efficiency.

  12. The eukaryotic RNA exosome.

    PubMed

    Januszyk, Kurt; Lima, Christopher D

    2014-02-01

    The eukaryotic RNA exosome is an essential multi-subunit ribonuclease complex that contributes to the degradation or processing of nearly every class of RNA in both the nucleus and cytoplasm. Its nine-subunit core shares structural similarity to phosphorolytic exoribonucleases such as bacterial PNPase. PNPase and the RNA exosome core feature a central channel that can accommodate single stranded RNA although unlike PNPase, the RNA exosome core is devoid of ribonuclease activity. Instead, the core associates with Rrp44, an endoribonuclease and processive 3'→5' exoribonuclease, and Rrp6, a distributive 3'→5' exoribonuclease. Recent biochemical and structural studies suggest that the exosome core is essential because it coordinates Rrp44 and Rrp6 recruitment, RNA can pass through the central channel, and the association with the core modulates Rrp44 and Rrp6 activities.

  13. Rapid avoidance acquisition in Wistar-Kyoto rats.

    PubMed

    Servatius, R J; Jiao, X; Beck, K D; Pang, K C H; Minor, T R

    2008-10-10

    The relationship between trait stress-sensitivity, avoidance acquisition and perseveration of avoidance was examined using male Wistar-Kyoto (WKY) and Sprague-Dawley (SD) rats. Behavior in an open field was measured prior to escape/avoidance (E/A) acquisition and extinction. E/A was assessed in a discrete trial lever-press protocol. The signal-shock interval was 60s with subsequent shocks delivered every 3s until a lever-press occurred. A 3-min flashing light safety signal was delivered contingent upon a lever-press (or failure to respond in 5 min). WKY rats displayed phenotypic low open field activity, but were clearly superior to SD rats in E/A performance. As avoidance responses were acquired and reached asymptotic performance, SD rats exhibited "warm up", that is, SD rats rarely made avoidance responses on the initial trial of a session, even though later trials were consistently accompanied with avoidance responses. In contrast, WKY rats did not show the "warm up" pattern and avoided on nearly all trials of a session including the initial trial. In addition to the superior acquisition of E/A, WKY rats demonstrated several other avoidance features that were different from SD rats. Although the rates of nonreinforced intertrial responses (ITRs) were relatively low and selective to the early safety period, WKY displayed more ITRs than SD rats. With removal of the shocks extinction was delayed in WKY rats, likely reflecting their nearly perfect avoidance performance. Even after extensive extinction, first trial avoidance and ITRs were evident in WKY rats. Thus, WKY rats have a unique combination of trait behavioral inhibition (low open field activity and stress sensitivity) and superior avoidance acquisition and response perseveration making this strain a good model to understand anxiety disorders.

  14. Single nucleotide RNA choreography.

    PubMed

    Hsiao, Chiaolong; Mohan, Srividya; Hershkovitz, Eli; Tannenbaum, Allen; Williams, Loren Dean

    2006-01-01

    New structural analysis methods, and a tree formalism re-define and expand the RNA motif concept, unifying what previously appeared to be disparate groups of structures. We find RNA tetraloops at high frequencies, in new contexts, with unexpected lengths, and in novel topologies. The results, with broad implications for RNA structure in general, show that even at this most elementary level of organization, RNA tolerates astounding variation in conformation, length, sequence and context. However the variation is not random; it is well-described by four distinct modes, which are 3-2 switches (backbone topology variations), insertions, deletions and strand clips.

  15. Quantum walks with tuneable self-avoidance in one dimension

    NASA Astrophysics Data System (ADS)

    Camilleri, Elizabeth; Rohde, Peter P.; Twamley, Jason

    2014-04-01

    Quantum walks exhibit many unique characteristics compared to classical random walks. In the classical setting, self-avoiding random walks have been studied as a variation on the usual classical random walk. Here the walker has memory of its previous locations and preferentially avoids stepping back to locations where it has previously resided. Classical self-avoiding random walks have found numerous algorithmic applications, most notably in the modelling of protein folding. We consider the analogous problem in the quantum setting - a quantum walk in one dimension with tunable levels of self-avoidance. We complement a quantum walk with a memory register that records where the walker has previously resided. The walker is then able to avoid returning back to previously visited sites or apply more general memory conditioned operations to control the walk. We characterise this walk by examining the variance of the walker's distribution against time, the standard metric for quantifying how quantum or classical a walk is. We parameterise the strength of the memory recording and the strength of the memory back-action on the walker, and investigate their effect on the dynamics of the walk. We find that by manipulating these parameters, which dictate the degree of self-avoidance, the walk can be made to reproduce ideal quantum or classical random walk statistics, or a plethora of more elaborate diffusive phenomena. In some parameter regimes we observe a close correspondence between classical self-avoiding random walks and the quantum self-avoiding walk.

  16. 16 CFR 300.6 - Labels to be avoided.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Labels to be avoided. 300.6 Section 300.6 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.6 Labels to be avoided. Stamps,...

  17. 28 CFR 552.23 - Confrontation avoidance procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Confrontation avoidance procedures. 552... MANAGEMENT CUSTODY Use of Force and Application of Restraints on Inmates § 552.23 Confrontation avoidance... information about the inmate and the immediate situation. Based on their assessment of that information,...

  18. 5 CFR 950.109 - Avoidance of conflict of interest.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Avoidance of conflict of interest. 950.109 Section 950.109 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE... PRIVATE VOLUNTARY ORGANIZATIONS General Provisions § 950.109 Avoidance of conflict of interest....

  19. Client Articulated Avoidance Goals in the Therapy Context

    ERIC Educational Resources Information Center

    Elliot, Andrew J.; Church, Marcy A.

    2002-01-01

    This research investigated the relationship between clients' pursuit of avoidance (relative to approach) therapy goals and change in subjective well-being (SWB) from the beginning to the end of therapy. Results indicated that clients with more avoidance therapy goals evidenced a smaller increase in SWB over the course of therapy than those with…

  20. Steering Clear: Avoidance in the Production of Idioms.

    ERIC Educational Resources Information Center

    Irujo, Suzanne

    A study of advanced English learners' use or avoidance of English idioms is described. The investigation looked at whether the subjects would attempt to use English idioms in a translation task containing idioms, or would instead use non-idiomatic synonyms or paraphrase. It also looked at which idioms are used and which are avoided: those that are…

  1. Educational Demands to Reduce Avoidance of Vocational Education in Jordan

    ERIC Educational Resources Information Center

    Alsa'aideh, Monim

    2016-01-01

    This study aims to identify the educational demands to reduce students' avoidance of vocational education in Jordan. Results of a previous study addressing reasons for avoidance of vocational education, distributed these reasons into personal, social, economic, educational and vocational domains. Focus-groups method was used to identify the…

  2. Passive Avoidance Is Linked to Impaired Fear Extinction in Humans

    ERIC Educational Resources Information Center

    Cornwell, Brian R.; Overstreet, Cassie; Krimsky, Marissa; Grillon, Christian

    2013-01-01

    Conventional wisdom dictates we must face our fears to conquer them. This idea is embodied in exposure-based treatments for anxiety disorders, where the intent of exposure is to reverse a history of avoidant behavior that is thought to fuel a patient's irrational fears. We tested in humans the relationship between fear and avoidance by combining…

  3. Experiential Avoidance and Problem Behavior: A Mediational Analysis

    ERIC Educational Resources Information Center

    Kingston, Jessica; Clarke, Sue; Remington, Bob

    2010-01-01

    Despite their formal dissimilarity, problem behaviors (e.g., substance misuse, binge eating, self-harm) may share a common function. According to Acceptance and Commitment Therapy (ACT), this shared function is "Experiential Avoidance," the process of avoiding, escaping or otherwise altering unwanted private events (e.g., thoughts, feelings,…

  4. Teacher Distress and the Role of Experiential Avoidance

    ERIC Educational Resources Information Center

    Hinds, Erika; Jones, Laura Backen; Gau, Jeffrey M.; Forrester, Kathleen K.; Biglan, Anthony

    2015-01-01

    Teachers' psychological well-being is important for teachers and students, but teaching is highly stressful, particularly in special education. We examined the role of experiential avoidance (EA) in the well-being of 529 middle and elementary school teachers. EA involves the tendency to avoid thoughts, feelings, and other internal experiences,…

  5. 47 CFR 74.803 - Frequency selection to avoid interference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SERVICES EXPERIMENTAL RADIO, AUXILIARY, SPECIAL BROADCAST AND OTHER PROGRAM DISTRIBUTIONAL SERVICES Low Power Auxiliary Stations § 74.803 Frequency selection to avoid interference. (a) Where two or more low... 47 Telecommunication 4 2011-10-01 2011-10-01 false Frequency selection to avoid interference....

  6. Safety Signals as Instrumental Reinforcers during Free-Operant Avoidance

    ERIC Educational Resources Information Center

    Fernando, Anushka B. P.; Urcelay, Gonzalo P.; Mar, Adam C.; Dickinson, Anthony; Robbins, Trevor W.

    2014-01-01

    Safety signals provide "relief" through predicting the absence of an aversive event. At issue is whether these signals also act as instrumental reinforcers. Four experiments were conducted using a free-operant lever-press avoidance paradigm in which each press avoided shock and was followed by the presentation of a 5-sec auditory safety…

  7. Geographic variation in potentially avoidable hospitalizations in France.

    PubMed

    Mercier, Gregoire; Georgescu, Vera; Bousquet, Jean

    2015-05-01

    Potentially avoidable hospitalizations are studied as an indirect measure of access to primary care. Understanding the determinants of these hospitalizations can help improve the quality, efficiency, and equity of health care delivery. Few studies have tackled the issue of potentially avoidable hospitalizations in France, and none has done so at the national level. We assessed disparities in potentially avoidable hospitalizations in France in 2012 and analyzed their determinants. The standardized rate of potentially avoidable hospitalizations ranged from 0.1 to 44.4 cases per 1,000 inhabitants, at the ZIP code level. Increased potentially avoidable hospitalizations were associated with higher mortality, lower density of acute care beds and ambulatory care nurses, lower median income, and lower education levels. This study unveils considerable variation in the rate of potentially avoidable hospitalizations in spite of France's mandatory, publicly funded health insurance system. In addition to epidemiological and sociodemographic factors, this study suggests that primary care organization plays a role in geographic disparities in potentially avoidable hospitalizations that might be addressed by increasing the number of nurses and enhancing team work in primary care. Policy makers should consider measuring potentially avoidable hospitalizations in France as an indicator of primary care organization.

  8. 14 CFR 125.224 - Collision avoidance system.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Collision avoidance system. 125.224 Section... Requirements § 125.224 Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part 125 must be equipped and operated according to the following table: Collision...

  9. 14 CFR 125.224 - Collision avoidance system.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Collision avoidance system. 125.224 Section... Requirements § 125.224 Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part 125 must be equipped and operated according to the following table: Collision...

  10. 14 CFR 125.224 - Collision avoidance system.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Collision avoidance system. 125.224 Section... Requirements § 125.224 Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part 125 must be equipped and operated according to the following table: Collision...

  11. 14 CFR 125.224 - Collision avoidance system.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Collision avoidance system. 125.224 Section... Requirements § 125.224 Collision avoidance system. Effective January 1, 2005, any airplane you operate under this part 125 must be equipped and operated according to the following table: Collision...

  12. Verbal Avoidance and Dissatisfaction in Intimate Conflict Situations

    ERIC Educational Resources Information Center

    Afifi, Tamara D.; McManus, Tara; Steuber, Keli; Coho, Amanda

    2009-01-01

    The purpose of this study was to more closely examine the association between avoidance and satisfaction during a potentially conflict-inducing conversation with one's dating partner. The results suggest that the way people respond to their own and their partner's conflict avoidance depends upon whether they are male or female. The perception of…

  13. 29 CFR 1912.4 - Avoidance of duplication.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 7 2010-07-01 2010-07-01 false Avoidance of duplication. 1912.4 Section 1912.4 Labor... (CONTINUED) ADVISORY COMMITTEES ON STANDARDS Organizational Matters § 1912.4 Avoidance of duplication. No... advisory committee established under section 7(b) of the Act....

  14. 49 CFR 1152.32 - Calculation of avoidable costs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... application will be used based on its geographical location. The railroad's total estimated system variable... to the branch on an actual expense basis. The avoidable costs of providing freight service on a... avoidable costs of providing freight service on a branch shall be just and reasonable, and shall not...

  15. The pineal complex in Roman high avoidance and Roman low avoidance rats.

    PubMed

    Seidel, A; Sousa Neto, J A; Huesgen, A; Vollrath, L; Manz, B; Gentsch, C; Lichtsteiner, M

    1990-01-01

    Previous studies have shown that the pineal gland of Roman high avoidance (RHA/Verh) rats is larger than that of Roman low avoidance rats (RLA/Verh). In the present study measurement of enzyme activities (serotonin-N-acetyl-transferase, hydroxyindole-O-methyltransferase) revealed that pineals of RHA/Verh rats are twice as active in melatonin production than pineals of RLA/Verh rats. Indoleamine content was also higher in RHA/Verh rats, whereas noradrenaline content was the same in both lines. When values were expressed per mg protein, these differences disappeared except for N-acetyl-serotonin and noradrenaline which were higher or lower in RHA/Verh rats, respectively. Both lines had higher serum levels of melatonin during the dark phase than during the light phase. However, RHA/Verh rats had increased serum levels as compared to RLA/Verh rats during both day and night. Morphometric analysis of the deep and superficial part of the pineal complex revealed, that the volumes of both parts are enlarged in RHA/Verh rats. Electron microscopic studies of pineals collected during day- and nighttime showed higher numbers of synaptic ribbons per unit area in pineals of RHA/Verh rats. In pineals collected during June synaptic ribbons displayed a day/night rhythm in RHA/Verh rats only, whereas in glands of both lines collected during November no daily changes were found. These results show that closely related but divergently selected rat lines may differ in pineal ultrastructure and pineal function.

  16. Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology.

    PubMed

    Shu, Dan; Li, Hui; Shu, Yi; Xiong, Gaofeng; Carson, William E; Haque, Farzin; Xu, Ren; Guo, Peixuan

    2015-10-27

    MicroRNAs play important roles in regulating the gene expression and life cycle of cancer cells. In particular, miR-21, an oncogenic miRNA is a major player involved in tumor initiation, progression, invasion and metastasis in several cancers, including triple negative breast cancer (TNBC). However, delivery of therapeutic miRNA or anti-miRNA specifically into cancer cells in vivo without collateral damage to healthy cells remains challenging. We report here the application of RNA nanotechnology for specific and efficient delivery of anti-miR-21 to block the growth of TNBC in orthotopic mouse models. The 15 nm therapeutic RNA nanoparticles contains the 58-nucleotide (nt) phi29 pRNA-3WJ as a core, a 8-nt sequence complementary to the seed region of miR-21, and a 39-nt epidermal growth factor receptor (EGFR) targeting aptamer for internalizing RNA nanoparticles into cancer cells via receptor mediated endocytosis. The RNase resistant and thermodynamically stable RNA nanoparticles remained intact after systemic injection into mice and strongly bound to tumors with little or no accumulation in healthy organs 8 h postinjection, and subsequently repressed tumor growth at low doses. The observed specific cancer targeting and tumor regression is a result of several key attributes of RNA nanoparticles: anionic charge which disallows nonspecific passage across negatively charged cell membrane; "active" targeting using RNA aptamers which increases the homing of RNA nanoparticles to cancer cells; nanoscale size and shape which avoids rapid renal clearance and engulfment by lung macrophages and liver Kupffer cells; favorable biodistribution profiles with little accumulation in healthy organs, which minimizes nonspecific side effects; and favorable pharmacokinetic profiles with extended in vivo half-life. The results demonstrate the clinical potentials of RNA nanotechnology based platform to deliver miRNA based therapeutics for cancer treatment.

  17. Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology

    PubMed Central

    2015-01-01

    MicroRNAs play important roles in regulating the gene expression and life cycle of cancer cells. In particular, miR-21, an oncogenic miRNA is a major player involved in tumor initiation, progression, invasion and metastasis in several cancers, including triple negative breast cancer (TNBC). However, delivery of therapeutic miRNA or anti-miRNA specifically into cancer cells in vivo without collateral damage to healthy cells remains challenging. We report here the application of RNA nanotechnology for specific and efficient delivery of anti-miR-21 to block the growth of TNBC in orthotopic mouse models. The 15 nm therapeutic RNA nanoparticles contains the 58-nucleotide (nt) phi29 pRNA-3WJ as a core, a 8-nt sequence complementary to the seed region of miR-21, and a 39-nt epidermal growth factor receptor (EGFR) targeting aptamer for internalizing RNA nanoparticles into cancer cells via receptor mediated endocytosis. The RNase resistant and thermodynamically stable RNA nanoparticles remained intact after systemic injection into mice and strongly bound to tumors with little or no accumulation in healthy organs 8 h postinjection, and subsequently repressed tumor growth at low doses. The observed specific cancer targeting and tumor regression is a result of several key attributes of RNA nanoparticles: anionic charge which disallows nonspecific passage across negatively charged cell membrane; “active” targeting using RNA aptamers which increases the homing of RNA nanoparticles to cancer cells; nanoscale size and shape which avoids rapid renal clearance and engulfment by lung macrophages and liver Kupffer cells; favorable biodistribution profiles with little accumulation in healthy organs, which minimizes nonspecific side effects; and favorable pharmacokinetic profiles with extended in vivo half-life. The results demonstrate the clinical potentials of RNA nanotechnology based platform to deliver miRNA based therapeutics for cancer treatment. PMID:26387848

  18. Use of behavioral avoidance testing in natural resource damage assessment

    USGS Publications Warehouse

    Lipton, J.; Little, E.E.; Marr, J.C.A.; DeLonay, A.J.; Bengston, David A.; Henshel, Diane S.

    1996-01-01

    Natural Resource Damage Assessment (NRDA) provisions established under federal and state statutes enable natural resource trustees to recover compensation from responsible parties to restore injured natural resources. Behavioral avoidance testing with fish has been used in NRDAs to determine injuries to natural resources and to establish restoration thresholds. In this manuscript we evaluate the use of avoidance testing to NRDA. Specifically, we discuss potential “acceptance criteria” to evaluate the applicability and relevance of avoidance testing. These acceptance criteria include: (1) regulatory relevance, (2) reproducibility of testing, (3) ecological significance, (4) quality assurance/quality control, and (5) relevance to restoration. We discuss each of these criteria with respect to avoidance testing. Overall, we conclude that avoidance testing can be an appropriate, defensible, and desirable aspect of an NRDA.

  19. Internet addiction symptoms, disordered eating, and body image avoidance.

    PubMed

    Rodgers, Rachel F; Melioli, Tiffany; Laconi, Stéphanie; Bui, Eric; Chabrol, Henri

    2013-01-01

    Internet addiction is an increasing concern among young adults. Self-presentational theory posits that the Internet offers a context in which individuals are able to control their image. Little is known about body image and eating concerns among pathological Internet users. The aim of this study was to explore the association between Internet addiction symptoms, body image esteem, body image avoidance, and disordered eating. A sample of 392 French young adults (68 percent women) completed an online questionnaire assessing time spent online, Internet addiction symptoms, disordered eating, and body image avoidance. Fourteen men (11 percent) and 26 women (9.7 percent) reported Internet addiction. Body image avoidance was associated with Internet addiction symptoms among both genders. Controlling for body-mass index, Internet addiction symptoms, and body image avoidance were both significant predictors of disordered eating among women. These findings support the self-presentational theory of Internet addiction and suggest that body image avoidance is an important factor.

  20. Vine tendrils use contact chemoreception to avoid conspecific leaves.

    PubMed

    Fukano, Yuya

    2017-03-15

    Movement and growth habit of climbing plants have attracted attention since the time of Charles Darwin; however, there are no reports on whether plants can choose suitable hosts or avoid unsuitable ones based on chemoreception. Here, I show that the tendrils of Cayratia japonica (Vitaceae) appear to avoid conspecific leaves using contact chemoreception for oxalates, which are highly concentrated in C. japonica leaves. The coiling experiments show that C. japonica has a flexible plastic response to avoid coiling around conspecific leaves. The coiling response is negatively correlated with the oxalate content in the contacted leaves. Experiments using laboratory chemicals indicate that the tendrils avoid oxalate-coated plastic sticks. These results indicate that the tendrils of C. japonica avoid coiling around a conspecific leaf based on contact chemoreception for oxalate compounds. The tendrils of climbing plants may function as a chemoreceptor system to detect the chemical cues of a contacted plant.

  1. How do walkers avoid a mobile robot crossing their way?

    PubMed

    Vassallo, Christian; Olivier, Anne-Hélène; Souères, Philippe; Crétual, Armel; Stasse, Olivier; Pettré, Julien

    2017-01-01

    Robots and Humans have to share the same environment more and more often. In the aim of steering robots in a safe and convenient manner among humans it is required to understand how humans interact with them. This work focuses on collision avoidance between a human and a robot during locomotion. Having in mind previous results on human obstacle avoidance, as well as the description of the main principles which guide collision avoidance strategies, we observe how humans adapt a goal-directed locomotion task when they have to interfere with a mobile robot. Our results show differences in the strategy set by humans to avoid a robot in comparison with avoiding another human. Humans prefer to give the way to the robot even when they are likely to pass first at the beginning of the interaction.

  2. Disgust as a motivator of avoidance of spiders.

    PubMed

    Woody, Sheila R; McLean, Carmen; Klassen, Tammy

    2005-01-01

    Individuals with small animal phobias show elevated general disgust sensitivity, and spider phobics often endorse both fear and disgust in response to a spider. Some researchers have argued that the link between disgust and fear of small animals is spurious. On the other hand, disgust may play a functional role, as might any negative emotion that is strongly stimulus-bound, in which escape or avoidance is negatively reinforced. It is therefore important to clarify whether disgust has a functional or epiphenomenal role in avoidance of feared stimuli. The present study examined the degree to which disgust motivates avoidance of spider-related stimuli using a series of behavioral avoidance tests comparing a harmless tarantula, a pen that had come in contact with the spider, and a clean pen. Peak disgust was a stronger predictor than anxiety of avoidance of both the spider and the "contaminated" pen.

  3. Neural substrates of approach-avoidance conflict decision-making

    PubMed Central

    Aupperle, Robin L.; Melrose, Andrew J.; Francisco, Alex; Paulus, Martin P.; Stein, Murray B.

    2014-01-01

    Animal approach-avoidance conflict paradigms have been used extensively to operationalize anxiety, quantify the effects of anxiolytic agents, and probe the neural basis of fear and anxiety. Results from human neuroimaging studies support that a frontal-striatal-amygdala neural circuitry is important for approach-avoidance learning. However, the neural basis of decision-making is much less clear in this context. Thus, we combined a recently developed human approach-avoidance paradigm with functional magnetic resonance imaging (fMRI) to identify neural substrates underlying approach-avoidance conflict decision-making. Fifteen healthy adults completed the approach-avoidance conflict (AAC) paradigm during fMRI. Analyses of variance were used to compare conflict to non-conflict (avoid-threat and approach-reward) conditions and to compare level of reward points offered during the decision phase. Trial-by-trial amplitude modulation analyses were used to delineate brain areas underlying decision-making in the context of approach/avoidance behavior. Conflict trials as compared to the non-conflict trials elicited greater activation within bilateral anterior cingulate cortex (ACC), anterior insula, and caudate, as well as right dorsolateral prefrontal cortex. Right caudate and lateral PFC activation was modulated by level of reward offered. Individuals who showed greater caudate activation exhibited less approach behavior. On a trial-by-trial basis, greater right lateral PFC activation related to less approach behavior. Taken together, results suggest that the degree of activation within prefrontal-striatal-insula circuitry determines the degree of approach versus avoidance decision-making. Moreover, the degree of caudate and lateral PFC activation is related to individual differences in approach-avoidance decision-making. Therefore, the AAC paradigm is ideally suited to probe anxiety-related processing differences during approach-avoidance decision-making. PMID:25224633

  4. Fabrication of Polyvalent Therapeutic RNA Nanoparticles for Specific Delivery of siRNA, Ribozyme and Drugs to Targeted Cells for Cancer Therapy

    PubMed Central

    Shu, Yi; Shu, Dan; Diao, Zhijuan; Shen, Guanxin; Guo, Peixuan

    2010-01-01

    Bacteriophage phi29 DNA packaging motor is geared by a six-pRNA ring. pRNA is able to form a multimeric complex and patterned superstructures via the interaction of two reengineered interlocking loops. This unique feature makes it an ideal polyvalent vehicle for nanomachine fabrication, pathogen detection, and the delivery of therapeutics. This report describes novel approaches for the fabrication of polyvalent therapeutic pRNA nanoparticles, especially tetramers for specific siRNA delivery to cancer cells and for the silencing of targeted genes. RNA 3-D design, circular permutation, folding energy alteration, and nucleotide modification were applied to generate stable RNA nanoparticles with low toxicity. Animal trials demonstrated the high efficiency of the polyvalent RNA nanoparticles in the prevention and treatment of cancer. Using such protein-free nanoparticles as therapeutic reagents would allow for long-term administration to avoid the induction of antibody due to repeated treatment for chronic diseases. PMID:21243099

  5. Tomato phyE Is Required for Shade Avoidance in the Absence of phyB1 and phyB2

    PubMed Central

    Schrager-Lavelle, Amanda; Herrera, Leslie A.; Maloof, Julin N.

    2016-01-01

    The phytochrome (phy) family of red and far-red photoreceptors provides plants with critical information about their surrounding environment and can signal downstream developmental and physiological changes. Neighboring plants compete for limited light resources, and their presence is detected by the phytochrome photoreceptors as a reduced ratio of red: far-red light. One common response to shade is increased elongation of petioles and internodes to compete with their neighbors. While the phytochrome family, phyB in particular, has been well studied in Arabidopsis, information about the other phytochrome family members is limited, especially in sympodial crop plants such as tomato, that have a very different architecture from that of the model plant. To study the tomato phytochrome family we took advantage of several existing mutants and generated an artificial miRNA (amiRNA) line to target SlPHYE, the remaining phytochrome B subfamily member with no currently available mutant line. Here, we characterize internode elongation and shade avoidance phenotypes of the SlPHYE amiRNA line (PHYE amiRNA). In addition, higher order phytochrome subfamily B mutants were generated with the PHYE amiRNA line to investigate the role of SlphyE within the phyB subfamily. We find that the PHYE amiRNA line has no detectable phenotype on its own, however in higher order combinations with SlphyB1 and/or SlphyB2 there are notable defects in shade avoidance. Most notably, we find that the triple mutant combination of SlPHYE amiRNA, SlphyB1, and SlphyB2 has a phenotype that is much stronger than the SlphyB1 SlphyB2 double, showing constitutive shade avoidance and little to no response to shade. This indicates that SlphyE is required for the shade avoidance response in the absence of SlphyB1 and SlphyB2. PMID:27695458

  6. Reduction in Memory Specificity Following an Approach/Avoidance Scrambled Sentences Task Relates to Cognitive Avoidant Coping

    ERIC Educational Resources Information Center

    Debeer, Elise; Raes, Filip; Williams, J. Mark G.; Hermans, Dirk

    2013-01-01

    "Overgeneral autobiographical memory" (OGM) refers to the tendency to retrieve less specific personal memories. According to the functional avoidance hypothesis, OGM might act as a cognitive strategy to avoid emotionally distressing details of negative memories. In the present study, we investigated the effect of an experimentally…

  7. On the role of subsecond dopamine release in conditioned avoidance

    PubMed Central

    Oleson, Erik B.; Cheer, Joseph F.

    2013-01-01

    Using shock avoidance procedures to study conditioned behavioral responses has a rich history within the field of experimental psychology. Such experiments led to the formulation of the general concept of negative reinforcement and specific theories attempting to explain escape and avoidance behavior, or why animals choose to either terminate or prevent the presentation of an aversive event. For example, the two-factor theory of avoidance holds that cues preceding an aversive event begin to evoke conditioned fear responses, and these conditioned fear responses reinforce the instrumental avoidance response. Current neuroscientific advances are providing new perspectives into this historical literature. Due to its well-established role in reinforcement processes and behavioral control, the mesolimbic dopamine system presented itself as a logical starting point in the search for neural correlates of avoidance and escape behavior. We recently demonstrated that phasic dopamine release events are inhibited by stimuli associated with aversive events but increased by stimuli preceding the successful avoidance of the aversive event. The latter observation is inconsistent with the second component of the two-factor theory of avoidance and; therefore, led us propose a new theoretical explanation of conditioned avoidance: (1) fear is initially conditioned to the warning signal and dopamine computes this fear association as a decrease in release, (2) the warning signal, now capable of producing a negative emotional state, suppresses dopamine release and behavior, (3) over repeated trials the warning signal becomes associated with safety rather than fear; dopaminergic neurons already compute safety as an increase in release and begin to encode the warning signal as the earliest predictor of safety (4) the warning signal now promotes conditioned avoidance via dopaminergic modulation of the brain's incentive-motivational circuitry. PMID:23759871

  8. Avoided-level-crossing statistics in open chaotic billiards.

    PubMed

    Poli, Charles; Dietz, Barbara; Legrand, Olivier; Mortessagne, Fabrice; Richter, Achim

    2009-09-01

    We investigate a two-level model with a large number of open decay channels in order to describe avoided level crossing statistics in open chaotic billiards. This model allows us to describe the fundamental changes in the probability distribution of the avoided level crossings compared with the closed case. Explicit expressions are derived for systems with preserved and broken time-reversal symmetry. We find that the decay process induces a modification at small spacings of the probability distribution of the avoided level crossings due to an attraction of the resonances. The theoretical predictions are in complete agreement with the recent experimental results of Dietz [Phys. Rev. E 73, 035201 (2006)].

  9. The Role of Allergen Exposure and Avoidance in Asthma

    PubMed Central

    Baxi, Sachin N.; Phipatanakul, Wanda

    2010-01-01

    Allergy testing and avoidance of allergens plays an important role in asthma control. Increased allergen exposure, in genetically susceptible individuals, can lead to allergic sensitization. Continued allergen exposure can increase the risk of asthma and other allergic diseases. In a patient with persistent asthma, identification of indoor and outdoor allergens and subsequent avoidance can improve symptoms. Often times, a patient will have multiple allergies and the avoidance plan should target all positive allergens. Several studies have shown that successful allergen remediation includes a comprehensive approach including education, cleaning, physical barriers and maintaining these practices. PMID:20568555

  10. Systems and Techniques for Identifying and Avoiding Ice

    NASA Technical Reports Server (NTRS)

    Hansman, R. John

    1995-01-01

    In-flight icing is one of the most difficult aviation weather hazards facing general aviation. Because most aircraft in the general aviation category are not certified for flight into known icing conditions, techniques for identifying and avoiding in-flight ice are important to maintain safety while increasing the utility and dispatch capability which is part of the AGATE vision. This report summarizes a brief study effort which: (1) Reviewed current ice identification, forecasting, and avoidance techniques; (2) Assessed feasibility of improved forecasting and ice avoidance procedures; and (3) Identified key issues for the development of improved capability with regard to in-flight icing.

  11. Assessing integrity of insect RNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Assessing total RNA integrity is important for the success of downstream RNA applications. The 2100 Bioanalyzer system with the RNA Integrity Number (RIN) provides a quantitative measure of RNA degradation. Although RINs may not be ascertained for RNA from all organisms, namely those with unusual or...

  12. RNA based evolutionary optimization

    NASA Astrophysics Data System (ADS)

    Schuster, Peter

    1993-12-01

    The notion of an RNA world has been introduced for a prebiotic scenario that is dominated by RNA molecules and their properties, in particular their capabilities to act as templates for reproduction and as catalysts for several cleavage and ligation reactions of polynucleotides and polypeptides. This notion is used here also for simple experimental assays which are well suited to study evolution in the test tube. In molecular evolution experiments fitness is determined in essence by the molecular structures of RNA molecules. Evidence is presented for adaptation to environment in cell-free media. RNA based molecular evolution experiments have led to interesting spin-offs in biotechnology, commonly called ‘applied molecular evolution’, which make use of Darwinian trial-and-error strategies in order to synthesize new pharmacological compounds and other advanced materials on a biological basis. Error-propagation in RNA replication leads to formation of mutant spectra called ‘quasispecies’. An increase in the error rate broadens the mutant spectrum. There exists a sharply defined threshold beyond which heredity breaks down and evolutionary adaptation becomes impossible. Almost all RNA viruses studied so far operate at conditions close to this error threshold. Quasispecies and error thresholds are important for an understanding of RNA virus evolution, and they may help to develop novel antiviral strategies. Evolution of RNA molecules can be studied and interpreted by considering secondary structures. The notion of sequence space introduces a distance between pairs of RNA sequences which is tantamount to counting the minimal number of point mutations required to convert the sequences into each other. The mean sensitivity of RNA secondary structures to mutation depends strongly on the base pairing alphabet: structures from sequences which contain only one base pair (GC or AU are much less stable against mutation than those derived from the natural (AUGC) sequences

  13. Ab initio RNA folding

    NASA Astrophysics Data System (ADS)

    Cragnolini, Tristan; Derreumaux, Philippe; Pasquali, Samuela

    2015-06-01

    RNA molecules are essential cellular machines performing a wide variety of functions for which a specific three-dimensional structure is required. Over the last several years, the experimental determination of RNA structures through x-ray crystallography and NMR seems to have reached a plateau in the number of structures resolved each year, but as more and more RNA sequences are being discovered, the need for structure prediction tools to complement experimental data is strong. Theoretical approaches to RNA folding have been developed since the late nineties, when the first algorithms for secondary structure prediction appeared. Over the last 10 years a number of prediction methods for 3D structures have been developed, first based on bioinformatics and data-mining, and more recently based on a coarse-grained physical representation of the systems. In this review we are going to present the challenges of RNA structure prediction and the main ideas behind bioinformatic approaches and physics-based approaches. We will focus on the description of the more recent physics-based phenomenological models and on how they are built to include the specificity of the interactions of RNA bases, whose role is critical in folding. Through examples from different models, we will point out the strengths of physics-based approaches, which are able not only to predict equilibrium structures, but also to investigate dynamical and thermodynamical behavior, and the open challenges to include more key interactions ruling RNA folding.

  14. Ab initio RNA folding.

    PubMed

    Cragnolini, Tristan; Derreumaux, Philippe; Pasquali, Samuela

    2015-06-17

    RNA molecules are essential cellular machines performing a wide variety of functions for which a specific three-dimensional structure is required. Over the last several years, the experimental determination of RNA structures through x-ray crystallography and NMR seems to have reached a plateau in the number of structures resolved each year, but as more and more RNA sequences are being discovered, the need for structure prediction tools to complement experimental data is strong. Theoretical approaches to RNA folding have been developed since the late nineties, when the first algorithms for secondary structure prediction appeared. Over the last 10 years a number of prediction methods for 3D structures have been developed, first based on bioinformatics and data-mining, and more recently based on a coarse-grained physical representation of the systems. In this review we are going to present the challenges of RNA structure prediction and the main ideas behind bioinformatic approaches and physics-based approaches. We will focus on the description of the more recent physics-based phenomenological models and on how they are built to include the specificity of the interactions of RNA bases, whose role is critical in folding. Through examples from different models, we will point out the strengths of physics-based approaches, which are able not only to predict equilibrium structures, but also to investigate dynamical and thermodynamical behavior, and the open challenges to include more key interactions ruling RNA folding.

  15. Isolation of an Asymmetric RNA Uncoating Intermediate for a Single-Stranded RNA Plant Virus

    PubMed Central

    Bakker, Saskia E.; Ford, Robert J.; Barker, Amy M.; Robottom, Janice; Saunders, Keith; Pearson, Arwen R.; Ranson, Neil A.; Stockley, Peter G.

    2012-01-01

    We have determined the three-dimensional structures of both native and expanded forms of turnip crinkle virus (TCV), using cryo-electron microscopy, which allows direct visualization of the encapsidated single-stranded RNA and coat protein (CP) N-terminal regions not seen in the high-resolution X-ray structure of the virion. The expanded form, which is a putative disassembly intermediate during infection, arises from a separation of the capsid-forming domains of the CP subunits. Capsid expansion leads to the formation of pores that could allow exit of the viral RNA. A subset of the CP N-terminal regions becomes proteolytically accessible in the expanded form, although the RNA remains inaccessible to nuclease. Sedimentation velocity assays suggest that the expanded state is metastable and that expansion is not fully reversible. Proteolytically cleaved CP subunits dissociate from the capsid, presumably leading to increased electrostatic repulsion within the viral RNA. Consistent with this idea, electron microscopy images show that proteolysis introduces asymmetry into the TCV capsid and allows initial extrusion of the genome from a defined site. The apparent formation of polysomes in wheat germ extracts suggests that subsequent uncoating is linked to translation. The implication is that the viral RNA and its capsid play multiple roles during primary infections, consistent with ribosome-mediated genome uncoating to avoid host antiviral activity. PMID:22306464

  16. Avoidance maneuevers selected while viewing cockpit traffic displays

    NASA Technical Reports Server (NTRS)

    Smith, J. D.; Ellis, S. R.; Lee, E.

    1982-01-01

    Ten airline pilots rates the collision danger of air traffic presented on cockpit displays of traffic information while they monitored simulated departures from Denver. They selected avoidance maneuvers when necessary for separation. Most evasive maneuvers were turns rather than vertical maneuvers. Evasive maneuvers chosen for encounters with low or moderate collision danger were generally toward the intruding aircraft. This tendency lessened as the perceived threat level increased. In the highest threst situations pilots turned toward the intruder only at chance levels. Intruders coming from positions in front of the pilot's own ship were more frequently avoided by turns toward than when intruders approached laterally or from behind. Some of the implications of the pilots' turning-toward tendencies are discussed with respect to automatic collision avoidance systems and coordination of avoidance maneuvers of conflicting aircraft.

  17. Does the Organized Sexual Murderer Better Delay and Avoid Detection?

    PubMed

    Beauregard, Eric; Martineau, Melissa

    2016-01-01

    According to the organized-disorganized model, organized sexual murderers adopt specific behaviors during the commission of their crimes that contribute to avoiding police detection. The current study examines the effect of sexual murderers' organized behaviors on their ability to both delay and/or avoid police detection. Using a combination of negative binomial and logistic regression analyses on a sample of 350 sexual murder cases, findings showed that although both measures of delaying and avoiding detection are positively correlated, different behavioral patterns were observed. For instance, offenders who moved the victim's body were more likely to avoid detection but the victim's body was likely to be recovered faster. Moreover, victim characteristics have an impact on both measures; however, this effect disappears for the measure of delaying detection once the organized behaviors are introduced. Implications of the findings are discussed.

  18. 31 CFR 800.104 - Transactions or devices for avoidance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (Continued) OFFICE OF INVESTMENT SECURITY, DEPARTMENT OF THE TREASURY REGULATIONS PERTAINING TO MERGERS, ACQUISITIONS, AND TAKEOVERS BY FOREIGN PERSONS General § 800.104 Transactions or devices for avoidance....

  19. Speak Up: Help Avoid Mistakes in Your Surgery

    MedlinePlus

    ... other medical facilities that are accredited by The Joint Commission must follow a procedure that helps surgeons avoid these mistakes. (Facilities that are accredited by The Joint Commission are listed on The Joint Commission’s Quality Check ...

  20. The brief experiential avoidance questionnaire: development and initial validation.

    PubMed

    Gámez, Wakiza; Chmielewski, Michael; Kotov, Roman; Ruggero, Camilo; Suzuki, Nadia; Watson, David

    2014-03-01

    The 62-item Multidimensional Experiential Avoidance Questionnaire (MEAQ) was recently developed to assess a broad range of experiential avoidance (EA) content. However, practical clinical and research considerations made a briefer measure of EA desirable. Using items from the original 62-item MEAQ, a 15-item scale was created that tapped content from each of the MEAQ's six dimensions. Items were selected on the basis of their performance in 3 samples: undergraduates (n = 363), psychiatric outpatients (n = 265), and community adults (n = 215). These items were then evaluated using 2 additional samples (314 undergraduates and 201 psychiatric outpatients) and cross-validated in 2 new, independent samples (283 undergraduates and 295 community adults). The resulting measure (Brief Experiential Avoidance Questionnaire; BEAQ) demonstrated good internal consistency. It also exhibited strong convergence with respect to each of the MEAQ's 6 dimensions. The BEAQ demonstrated expected associations with measures of avoidance, psychopathology, and quality of life and was distinguishable from negative affectivity and neuroticism.

  1. 16 CFR 301.28 - Labels to be avoided.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.28 Labels to be avoided. Labels which are insecurely or inconspicuously attached, or which in the course of offering the fur product for sale,...

  2. 16 CFR 301.28 - Labels to be avoided.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.28 Labels to be avoided. Labels which are insecurely or inconspicuously attached, or which in the course of offering the fur product for sale,...

  3. 16 CFR 301.28 - Labels to be avoided.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.28 Labels to be avoided. Labels which are insecurely or inconspicuously attached, or which in the course of offering the fur product for sale,...

  4. 16 CFR 301.28 - Labels to be avoided.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.28 Labels to be avoided. Labels which are insecurely or inconspicuously attached, or which in the course of offering the fur product for sale,...

  5. 16 CFR 301.28 - Labels to be avoided.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.28 Labels to be avoided. Labels which are insecurely or inconspicuously attached, or which in the course of offering the fur product for sale,...

  6. Effects of Stimulation Intensity on Sociopathic Avoidance Learning

    ERIC Educational Resources Information Center

    Chesno, Frank A.; Kilmann, Peter R.

    1975-01-01

    The purpose of this study was to propose an alternative explanation of defective sociopathic avoidance conditioning based on the model of sensory reinforcement (Duffy, 1962) and the principle of optimal stimulation (Leuba, 1955). (Author)

  7. Summary of avoidable cancers in the Nordic countries.

    PubMed

    Olsen, J H; Andersen, A; Dreyer, L; Pukkala, E; Tryggvadottir, L; Gerhardsson de Verdier, M; Winther, J F

    1997-01-01

    An overview is given of the most important known causes of cancer in the five Nordic countries and the resulting number of cancers that are potentially avoidable. The main causes include active and passive smoking, alcohol consumption, exposure to asbestos and other occupational carcinogens, solar and ionizing radiation, obesity, human papillomavirus infection in the female genital tract and infection with Helicobacter pylori. The organs most commonly affected are those of the respiratory system, the upper digestive tract and stomach, skin, the lower urinary tract and the uterine cervix. Annually, more than 18,000 cancers in men and 11,000 in women in the Nordic populations could be avoided by eliminating exposure to known carcinogens which is equivalent to 33% and 20% of all cancers arising in men and women, respectively, around the year 2000. Smoking habits account for a little more than half of these avoidable cases. Estimates of avoidable cancers are given for each Nordic country, separately.

  8. How You Can Help Your Child Avoid & Address Bullying

    MedlinePlus

    ... How You Can Help Your Child Avoid & Address Bullying Page Content Article Body Whether on the school ... other ways. Getting that response usually makes the bullying behavior continue. Your child should try to keep ...

  9. 20 CFR 606.24 - Application for avoidance.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... THE FEDERAL UNEMPLOYMENT TAX ACT; ADVANCES UNDER TITLE XII OF THE SOCIAL SECURITY ACT Relief From Tax... State law changes, effective for the taxable year for which the avoidance is requested, which...

  10. NONCONSCIOUS EFFECTS OF POWER ON BASIC APPROACH AND AVOIDANCE TENDENCIES

    PubMed Central

    Smith, Pamela K.; Bargh, John A.

    2008-01-01

    According to the approach/inhibition theory of power (Keltner, Gruenfeld, & Anderson, 2003), having power should be associated with the approach system, and lacking power with the avoidance system. However, to this point research has focused solely on whether power leads to more action, particularly approach-related action, or not. In three experiments, we extend this research by exploring the direct, unintentional relation between power and both approach and avoidance tendencies. Priming high power led to greater relative BAS strength than priming low power, but did not affect the BIS (Exp. 1). High-power priming also facilitated both simple and complex approach behavior, but did not affect avoidance behavior (Exp. 2−3). These effects of power occurred even in power-irrelevant situations. They also cannot be explained by priming of general positive versus negative constructs, nor by changes in positive, negative, approach-related, or avoidance-related affect. PMID:18568085

  11. Multiple-spacecraft reconfigurations through collision avoidance, bouncing, and stalemate

    NASA Technical Reports Server (NTRS)

    Kim, Y.; Mesbahi, M.; Hadaegh, F. Y.

    2004-01-01

    We consider constrained multiple-spacecraft reconfigurations outside of a gravity well in deep space. As opposed to the single-spacecraft scenario, such reconfigurations involve collision avoidance constraints that can be formalized and embedded in a nonconvex.

  12. Pregnant Women Should Avoid Zika-Hit Texas Town: CDC

    MedlinePlus

    ... news/fullstory_162573.html Pregnant Women Should Avoid Zika-Hit Texas Town: CDC Advisory follows reports of ... border with Mexico, because five cases of local Zika infection have been reported there, U.S. health officials ...

  13. The Use of Arbitration to Avoid Litigation Under ERISA

    ERIC Educational Resources Information Center

    Donaldson, Richard P.

    1975-01-01

    In this symposium report it is suggested that arbitration can be used to avoid litigation of ERISA pension and welfare benefit claims if the negotiated plan or related collective bargaining agreement provides for arbitration or benefit disputes. (Author/LBH)

  14. 28 CFR 552.23 - Confrontation avoidance procedures.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... MANAGEMENT CUSTODY Use of Force and Application of Restraints on Inmates § 552.23 Confrontation avoidance... knowledge they have gained about the inmate and the incident, determine if use of force is necessary....

  15. The Color Red Supports Avoidance Reactions to Unhealthy Food.

    PubMed

    Rohr, Michaela; Kamm, Friederike; Koenigstorfer, Joerg; Groeppel-Klein, Andrea; Wentura, Dirk

    2015-01-01

    Empirical evidence suggests that the color red acts like an implicit avoidance cue in food contexts. Thus specific colors seem to guide the implicit evaluation of food items. We built upon this research by investigating the implicit meaning of color (red vs. green) in an approach-avoidance task with healthy and unhealthy food items. Thus, we examined the joint evaluative effects of color and food: Participants had to categorize food items by approach-avoidance reactions, according to their healthfulness. Items were surrounded by task-irrelevant red or green circles. We found that the implicit meaning of the traffic light colors influenced participants' reactions to the food items. The color red (compared to green) facilitated automatic avoidance reactions to unhealthy foods. By contrast, approach behavior toward healthy food items was not moderated by color. Our findings suggest that traffic light colors can act as implicit cues that guide automatic behavioral reactions to food.

  16. Avoid Counterfeit Pesticide Products for Dogs and Cats

    EPA Pesticide Factsheets

    EPA is aware of counterfeit pet pesticides designed to look like legitimately registered pesticide products. The information on this page is intended to help consumers avoid unregistered pet products.

  17. Food allergy: is strict avoidance the only answer?

    PubMed

    Allen, C W; Campbell, D E; Kemp, A S

    2009-08-01

    It is an immunological paradigm that avoidance of food allergen may reduce the risk or prevent immunological reactions and conversely that a greater exposure increases the magnitude of the immune response. Consequently, food allergen avoidance has been recommended to reduce the risk of sensitization in infants and to prevent clinical reactions in children with positive skin prick tests (SPT). In the latter setting, it is hoped that avoidance may either promote or at least not retard the development of tolerance. Animal studies, however, have demonstrated that tolerance to food allergens may be induced by either large (high zone tolerance) or small (low zone tolerance) doses, whereas doses in between may actually stimulate immune responses. In this review, we discuss whether strict allergen avoidance is always the most appropriate strategy for preventing or managing IgE-mediated food allergy.

  18. Who discovered messenger RNA?

    PubMed

    Cobb, Matthew

    2015-06-29

    The announcement of the discovery of messenger RNA (mRNA) and the cracking of the genetic code took place within weeks of each other in a climax of scientific excitement during the summer of 1961. Although mRNA is of decisive importance to our understanding of gene function, no Nobel Prize was awarded for its discovery. The large number of people involved, the complex nature of the results, and the tortuous path that was taken over half a century ago, all show that simple claims of priority may not reflect how science works.

  19. Assessing cellular and circulating miRNA recovery: the impact of the RNA isolation method and the quantity of input material.

    PubMed

    El-Khoury, Victoria; Pierson, Sandrine; Kaoma, Tony; Bernardin, François; Berchem, Guy

    2016-01-20

    MicroRNAs (miRNAs) have emerged as promising cancer biomarkers. However, exploiting their informative potential requires careful optimization of their detection. Here, we compared the efficiency of commonly used RNA extraction kits in miRNA recovery from cells, plasma and urine/plasma-derived exosomes, using single-gene RT-qPCR and miRNA profiling. We used increasing amounts of starting material to investigate the impact of the input material size on miRNA extraction. We showed that miRNA recovery was largely influenced by the isolation method and by the amount of input material. In particular, the miRCURY™ kit provided highly pure RNA. However, its columns poorly recovered miRNAs from limiting amounts of cells and plasma, and rapidly saturated by large RNA species and plasma components, thus impeding miRNA recovery from high input amounts. Overall, the miRNeasy® kit permitted a better miRNA detection despite a less pure extracted RNA. Nevertheless, some miRNAs were preferentially or exclusively isolated by either of the methods. Trizol® LS resulted in very low purity RNA which affected RT-qPCR efficiency. In general, miRCURY™ biofluids kit efficiently extracted miRNAs from plasma. A careful selection of the RNA isolation method and the consideration of the type and size of input material are highly recommended to avoid biased results.

  20. In silico detection of tRNA sequence features characteristic to aminoacyl-tRNA synthetase class membership

    PubMed Central

    Jakó, Éena; Ittzés, Péter; Szenes, Áron; Kun, Ádám; Szathmáry, Eörs; Pál, Gábor

    2007-01-01

    Aminoacyl tRNA synthetases (aaRS) are grouped into Class I and II based on primary and tertiary structure and enzyme properties suggesting two independent phylogenetic lineages. Analogously, tRNA molecules can also form two respective classes, based on the class membership of their corresponding aaRS. Although some aaRS–tRNA interactions are not extremely specific and require editing mechanisms to avoid misaminoacylation, most aaRS–tRNA interactions are rather stereospecific. Thus, class-specific aaRS features could be mirrored by class-specific tRNA features. However, previous investigations failed to detect conserved class-specific nucleotides. Here we introduce a discrete mathematical approach that evaluates not only class-specific ‘strictly present’, but also ‘strictly absent’ nucleotides. The disjoint subsets of these elements compose a unique partition, named extended consensus partition (ECP). By analyzing the ECP for both Class I and II tDNA sets from 50 (13 archaeal, 30 bacterial and 7 eukaryotic) species, we could demonstrate that class-specific tRNA sequence features do exist, although not in terms of strictly conserved nucleotides as it had previously been anticipated. This finding demonstrates that important information was hidden in tRNA sequences inaccessible for traditional statistical methods. The ECP analysis might contribute to the understanding of tRNA evolution and could enrich the sequence analysis tool repertoire. PMID:17704131

  1. Reactive Collision Avoidance of UAVs withStereovision Sensing

    DTIC Science & Technology

    2014-01-17

    Instead, a minimum effort guidance ( MEG ) approach minimizes the control effort for the entire trajectory along with avoiding collisions for multiple...targets [17]. A collision cone approach [41] is used to detect potential collisions by considering a threat boundary around the obstacle in MEG guidance...It has been demonstrated that MEG is more suitable than PN [17]. However, collision avoidance problems do not have minimum effort requirements and

  2. Collision Avoidance Functional Requirements for Step 1. Revision 6

    NASA Technical Reports Server (NTRS)

    2006-01-01

    This Functional Requirements Document (FRD) describes the flow of requirements from the high level operational objectives down to the functional requirements specific to cooperative collision avoidance for high altitude, long endurance unmanned aircraft systems. These are further decomposed into performance and safety guidelines that are backed up by analysis or references to various documents or research findings. The FRD should be considered when establishing future policies, procedures, and standards pertaining to cooperative collision avoidance.

  3. Managed care contracting and payment reform: avoiding a showdown.

    PubMed

    Nugent, Michael E

    2010-07-01

    Health reform promises to fundamentally change what and how CMS and commercial payers reimburse providers. Providers need to transition from their traditionally adversarial, transactions-based payer relationships to ones that optimize purchaser and patient value for the dollar. To avoid negotiation table showdowns and to prepare for reform, commercial payers and providers should take three actions: Recognize the dead ends with their historical relationships. Formulate their road map to value-based contracting. Avoid operational pot-holes along the way.

  4. Avoiding Another Hollow Force: Optimizing the Joint Force Despite Sequestration

    DTIC Science & Technology

    2013-04-01

    Acquisition Reform Act. Congressional effort to improve the way major weapon systems are acquired and to avoid further cost overruns on such...Congress passed the Weapon Systems Acquisition Reform Act (WSARA) in an effort to improve the way major weapon systems are acquired and to avoid...While these key allies add strength to the western alliance, they also add potential fiscal burdens for the United States. With dozens of formal

  5. Incorporation of Tropical Cyclone Avoidance Into Automated Ship Scheduling

    DTIC Science & Technology

    2014-06-01

    TROPICAL CYCLONE AVOIDANCE INTO AUTOMATED SHIP SCHEDULING by Stephen W. Lantz June 2014 Thesis Advisor: Walter DeGrange Co-Advisor: Eva...COVERED Master’s Thesis 4. TITLE AND SUBTITLE INCORPORATION OF TROPICAL CYCLONE AVOIDANCE INTO AUTOMATED SHIP SCHEDULING 5. FUNDING NUMBERS 6... cyclones (TCs) frequently disrupt these plans, requiring diversions and inefficient steaming speeds. We evaluate the impact of adding anticipated TC

  6. Safety signals as instrumental reinforcers during free-operant avoidance

    PubMed Central

    Urcelay, Gonzalo P.; Mar, Adam C.; Dickinson, Anthony; Robbins, Trevor W.

    2014-01-01

    Safety signals provide “relief” through predicting the absence of an aversive event. At issue is whether these signals also act as instrumental reinforcers. Four experiments were conducted using a free-operant lever-press avoidance paradigm in which each press avoided shock and was followed by the presentation of a 5-sec auditory safety signal. When given a choice between two levers in Experiment 1, both avoiding shock, rats preferentially responded on the lever that produced the safety signal as feedback, even when footshock was omitted. Following avoidance training with a single lever in Experiment 2, removal of the signal led to a decrease in avoidance responses and an increase in responses during the safety period normally denoted by the signal. These behavioral changes demonstrate the dual conditioned reinforcing and fear inhibiting properties of the safety signal. The associative processes that support the reinforcing properties of a safety signal were tested using a novel revaluation procedure. Prior experience of systemic morphine during safety signal presentations resulted in an increased rate of avoidance responses to produce the safety signal during a drug-free extinction test, a finding not seen with d-amphetamine in Experiment 3. Morphine revaluation of the safety signal was repeated in Experiment 4 followed by a drug-free extinction test in which responses did not produce the signal for the first 10 min of the session. Instrumental avoidance in the absence of the signal was shown to be insensitive to prior signal revaluation, suggesting that the signal reinforces free-operant avoidance behavior through a habit-like mechanism. PMID:25135197

  7. The contribution of an avoidance response to contralateral neglect.

    PubMed

    Kodsi, Matthew H; Heilman, Kenneth M

    2002-06-11

    Displacement of line bisection to the right of midline in a patient with a right hemisphere lesion has been attributed to either contralesional attentional or intentional hemispatial neglect. However, it has been suggested that patients with hemispheric lesions might also have an avoidance response, and the ipsilesional eye and hand deviation attributed to neglect might, at least in part, be related to this response. The authors report a patient with a net contralateral neglect produced by the combination of intention and avoidance factors.

  8. Impact of Tactical and Strategic Weather Avoidance on Separation Assurance

    NASA Technical Reports Server (NTRS)

    Refai, Mohamad S.; Windhorst, Robert

    2011-01-01

    The ability to keep flights away from weather hazards while maintaining aircraft-to-aircraft separation is critically important. The Advanced Airspace Concept is an automation concept that implements a ground-based strategic conflict resolution algorithm for management of aircraft separation. The impact of dynamic and uncertain weather avoidance on this concept is investigated. A strategic weather rerouting system is integrated with the Advanced Airspace Concept, which also provides a tactical weather avoidance algorithm, in a fast time simulation of the Air Transportation System. Strategic weather rerouting is used to plan routes around weather in the 20 minute to two-hour time horizon. To address forecast uncertainty, flight routes are revised at 15 minute intervals. Tactical weather avoidance is used for short term trajectory adjustments (30 minute planning horizon) that are updated every minute to address any weather conflicts (instances where aircraft are predicted to pass through weather cells) that are left unresolved by strategic weather rerouting. The fast time simulation is used to assess the impact of tactical weather avoidance on the performance of automated conflict resolution as well as the impact of strategic weather rerouting on both conflict resolution and tactical weather avoidance. The results demonstrate that both tactical weather avoidance and strategic weather rerouting increase the algorithm complexity required to find aircraft conflict resolutions. Results also demonstrate that tactical weather avoidance is prone to higher airborne delay than strategic weather rerouting. Adding strategic weather rerouting to tactical weather avoidance reduces total airborne delays for the reported scenario by 18% and reduces the number of remaining weather violations by 13%. Finally, two features are identified that have proven important for strategic weather rerouting to realize these benefits; namely, the ability to revise reroutes and the use of maneuvers

  9. Avoidance of selenium-treated food by mallards

    USGS Publications Warehouse

    Heinz, G.H.; Sanderson, C.J.

    1990-01-01

    Adult, male mallards (Anas platyrhynchos) were given a choice between a control diet and a diet containing 5, 10 or 20 ppm selenium as selenomethionine dissolved in water and mixed into the diet. At 10 and 20 ppm, selenium-treated diets were avoided. Avoidance appeared to be caused by a conditioned response, probably to illness caused by the selenium and not to an aversion to the taste of the selenium.

  10. Use of the Atmospheric Electric Field for Terrain Avoidance

    DTIC Science & Technology

    1975-04-01

    realistic enough to yield results applicable in the real world? And if the po- tential gradients are found to be as predicted b,,r Poisson’s equation...electric field is believed to be realistic "since the application of the atmospheric electric field for stabilization or terrain avoidance will be...phenomena) that causes the fluctuations in atmospheric potential (or potential gradient) that makes terrain avoidance or any other application of the

  11. Avoidance strategies in an exceptional child during unsuccessful reading performances.

    PubMed

    Damico, Jack S; Nelson, Ryan L; Damico, Holly; Abendroth, Kathleen; Scott, Jennifer

    2008-01-01

    Employing an interactional analysis and focusing on the complexity of meaning making, this study investigates the uses of several types of avoidance strategies generated by a language-disordered boy who is struggling with literacy. The results suggest that these avoidance strategies may function as compensatory adaptations that assist him in overcoming his literacy limitations so that he can still sustain effective social action even within contexts where his literacy difficulties are highlighted. There are both theoretical and practical implications for these findings.

  12. Global and local obstacle avoidance technique for an autonomous vehicle

    NASA Astrophysics Data System (ADS)

    Gray, Keith W.; Saunders, Kevin S.

    1999-07-01

    The Center for Self-Organizing and Intelligent Systems (CSOIS) is engaged in developing autonomous ground vehicles. A significant problem for such vehicles is obstacle detection and avoidance. After studying various methods of detection, a scanning laser system was chosen that can detect objects at a distance of up to thirty feet while traveling between five and ten miles per hour. Once an object is detected, the vehicle must avoid it. The project employs a mission-level path planner that predetermines the path of a vehicle. One avoidance scheme is to inform the path planner of the obstacle and then let it re-plan the path. This is the global approach to the problem, which allows the use of existing software for maneuvering the vehicle. However, replanning is time consuming and lacks knowledge of the entire obstacle. An alternative approach is to use local avoidance, whereby a vehicle determines how to get by an obstacle without help from the path planner. This approach offers faster response without requiring the computing resource of the path planner. The disadvantage is that during local avoidance the vehicle ignores the global map of known obstacles and does not know to turn control back to the path planner if mission efficiency is adversely affected. This paper will describe a method for combining the current global path planner with a local obstacle avoidance technique to efficiently complete required tasks in a partially unknown environment.

  13. Chloroplasts can move in any direction to avoid strong light.

    PubMed

    Tsuboi, Hidenori; Wada, Masamitsu

    2011-01-01

    Chloroplasts migrate in response to different light intensities. Under weak light, chloroplasts gather at an illuminated area to maximize light absorption and photosynthesis rates (the accumulation response). In contrast, chloroplasts escape from strong light to avoid photodamage (the avoidance response). Photoreceptors involved in these phenomena have been identified in Arabidopsis thaliana and Adiantum capillus-veneris. Chloroplast behavior has been studied in detail during the accumulation response, but not for the avoidance response. Hence, we analyzed the chloroplast avoidance response in detail using dark-adapted Adiantum capillus-veneris gametophyte cells and partial cell irradiation with a microbeam of blue light. Chloroplasts escaped from an irradiated spot. Both duration of this response and the distance of the migrated chloroplasts were proportional to the total fluence irradiated. The speed of movement during the avoidance response was dependent on the fluence rate, but the speed of the accumulation response towards the microbeam from cell periphery was constant irrespective of fluence rate. When a chloroplast was only partially irradiated with a strong microbeam, it moved away towards the non-irradiated region within a few minutes. During this avoidance response two additional microbeam irradiations were applied to different locus of the same chloroplast. Under these conditions the chloroplast changed the moving direction after a lag time of a few minutes without rolling. Taken together, these findings indicate that chloroplasts can move in any direction and never have an intrinsic polarity. Similar phenomenon was observed in chloroplasts of Arabidopsis thaliana palisade cells.

  14. Introducing the Date and Acquaintance Rape Avoidance Scale.

    PubMed

    Resendez, Josephine R; Hughes, Jamie S

    2016-01-01

    We present the Date and Acquaintance Rape Avoidance Scale (DARAS). The DARAS is a measure of a woman's behaviors used to avoid date and acquaintance rape. Three factor structures were possible. The DARAS may have measured several factors related to alcohol and drug use, self-defense, and date behaviors; 2 factors related to behaviors to avoid acquaintance versus date rape; or a single factor that represented general vigilance. The data revealed a highly reliable, 63 item single factor that was correlated with stranger rape avoidance, rejection of rape myths, hostile sexist beliefs about men, and benevolent sexist beliefs about women. The creation of the DARAS adds to the growing body of research on rape avoidance. The DARAS is key to understanding the behaviors women employ to avoid date rape. Rather than placing the responsibility for rape on the victim, the DARAS was developed as a theoretical and applied tool that can be used to improve theory and construct rape education and prevention programs.

  15. Slow maturation of planning in obstacle avoidance in humans

    PubMed Central

    Corporaal, Sharissa H. A.; Duysens, Jacques; Bruijn, Sjoerd M.

    2015-01-01

    Complex gait (e.g., obstacle avoidance) requires a higher cognitive load than simple steady-state gait, which is a more automated movement. The higher levels of the central nervous system, responsible for adjusting motor plans to complex gait, develop throughout childhood into adulthood. Therefore, we hypothesize that gait strategies in complex gait are likely to mature until adulthood as well. However, little is known about the maturation of complex gait from childhood into adolescence and adulthood. To address this issue, we investigated obstacle avoidance in forty-four 8- to 18-yr-old participants who walked at preferred speed along a 6-m walkway on which a planar obstacle (150% of step length, 1 m wide) was projected. Participants avoided the obstacle by stepping over this projection, while lower body kinematics were recorded. Results showed that step length and speed adjustments during successful obstacle avoidance were similar across all ages, even though younger children modified step width to a greater extent. Additionally, the younger children used larger maximal toe elevations and take-off distances than older children. Moreover, during unsuccessful trials, younger children deployed exaggerated take-off distances, which resulted in obstacle contact upon the consecutive heel strike. These results indicate that obstacle avoidance is not fully matured in younger children, and that the inability to plan precise foot placements is an important factor contributing to failures in obstacle avoidance. PMID:26561604

  16. Radar-based collision avoidance for unmanned surface vehicles

    NASA Astrophysics Data System (ADS)

    Zhuang, Jia-yuan; Zhang, Lei; Zhao, Shi-qi; Cao, Jian; Wang, Bo; Sun, Han-bing

    2016-12-01

    Unmanned surface vehicles (USVs) have become a focus of research because of their extensive applications. To ensure safety and reliability and to perform complex tasks autonomously, USVs are required to possess accurate perception of the environment and effective collision avoidance capabilities. To achieve these, investigation into realtime marine radar target detection and autonomous collision avoidance technologies is required, aiming at solving the problems of noise jamming, uneven brightness, target loss, and blind areas in marine radar images. These technologies should also satisfy the requirements of real-time and reliability related to high navigation speeds of USVs. Therefore, this study developed an embedded collision avoidance system based on the marine radar, investigated a highly real-time target detection method which contains adaptive smoothing algorithm and robust segmentation algorithm, developed a stable and reliable dynamic local environment model to ensure the safety of USV navigation, and constructed a collision avoidance algorithm based on velocity obstacle (V-obstacle) which adjusts the USV's heading and speed in real-time. Sea trials results in multi-obstacle avoidance firstly demonstrate the effectiveness and efficiency of the proposed avoidance system, and then verify its great adaptability and relative stability when a USV sailing in a real and complex marine environment. The obtained results will improve the intelligent level of USV and guarantee the safety of USV independent sailing.

  17. Performance of humans in concurrent avoidance/positive-reinforcement schedules.

    PubMed

    Ruddle, H V; Bradshaw, C M; Szabadi, E; Foster, T M

    1982-07-01

    Performance maintained under concurrent schedules consisting of a variable-interval avoidance component and a variable-interval positive-reinforcement component was studied in three human subjects using points exchangeable for money as the reinforcer. The rate of responding in the avoidance component increased, and the rate of responding in the positive-reinforcement component declined, as a function of the frequency of point-losses avoided in the avoidance component. The performance of all three subjects conformed to equations proposed by Herrnstein to describe behavior in concurrent schedules. The logarithms of the ratios of the response rates in the two components, and the logarithms of the ratios of the times spent in the two components, were linearly related to the logarithms of the ratios of the frequency of loss avoidance in the avoidance component to the frequency of reinforcement in the positive-reinforcement component. When a changeover delay of 5.0 sec was imposed, the slopes of the linear functions were close to 1.0 in the case of two subjects, whereas the third subject exhibited significant undermatching. For two subjects the changeover delay was then reduced to 2.0 sec; in both cases the slopes of the linear functions were lower than under the 5.0-sec condition. One subject participated in a third phase, in which no changeover delay was imposed; there was a further reduction in the slopes of the linear functions.

  18. Sensor-Based Collision Avoidance: Theory and Experiments

    NASA Technical Reports Server (NTRS)

    Seraji, Homayoun; Steele, Robert; Ivlev, Robert

    1996-01-01

    A new on-line control strategy for sensor-based collision avoidance of manipulators and supporting experimental results are presented in this article. This control strategy is based on nullification of virtual forces applied to the end-effector by a hypothetical spring-plus-damper attached to the object's surface. In the proposed approach, the real-time arm control software continuously monitors the object distance measured by the arm-mounted proximity sensors. When this distance is less than a preset threshold, the collision avoidance control action is initiated to inhibit motion toward the object and thus prevent collision. This is accomplished by employing an outer feedback loop to perturb the end-effector nominal motion trajectory in real-time based on the sensory data. The perturbation is generated by a proportional-plus-integral (PI) collision avoidance controller acting on the difference between the sensed distance and the preset threshold. This approach is computationally very fast, requires minimal modification to the existing manipulator positioning system, and provides the manipulator with an on-line collision avoidance capability to react autonomously and intelligently. A dexterous RRC robotic arm is instrumented with infrared proximity sensors and is operated under the proposed collision avoidance strategy. Experimental results are presented to demonstrate end-effector collision avoidance both with an approaching object and while reaching inside a constricted opening.

  19. Hybrid Long-Range Collision Avoidance for Crowd Simulation.

    PubMed

    Golas, Abhinav; Narain, Rahul; Curtis, Sean; Lin, Ming C

    2014-07-01

    Local collision avoidance algorithms in crowd simulation often ignore agents beyond a neighborhood of a certain size. This cutoff can result in sharp changes in trajectory when large groups of agents enter or exit these neighborhoods. In this work, we exploit the insight that exact collision avoidance is not necessary between agents at such large distances, and propose a novel algorithm for extending existing collision avoidance algorithms to perform approximate, long-range collision avoidance. Our formulation performs long-range collision avoidance for distant agent groups to efficiently compute trajectories that are smoother than those obtained with state-of-the-art techniques and at faster rates. Comparison to real-world data demonstrates that crowds simulated with our algorithm exhibit an improved speed sensitivity to density similar to human crowds. Another issue often sidestepped in existing work is that discrete and continuum collision avoidance algorithms have different regions of applicability. For example, low-density crowds cannot be modeled as a continuum, while high-density crowds can be expensive to model using discrete methods. We formulate a hybrid technique for crowd simulation which can accurately and efficiently simulate crowds at any density with seamless transitions between continuum and discrete representations. Our approach blends results from continuum and discrete algorithms, based on local density and velocity variance. In addition to being robust across a variety of group scenarios, it is also highly efficient, running at interactive rates for thousands of agents on portable systems.

  20. Hybrid Long-Range Collision Avoidance for Crowd Simulation.

    PubMed

    Golas, Abhinav; Narain, Rahul; Curtis, Sean; Lin, Ming C

    2013-09-26

    Local collision avoidance algorithms in crowd simulation often ignore agents beyond a neighborhood of a certain size. This cutoff can result in sharp changes in trajectory when large groups of agents enter or exit these neighborhoods. In this work, we exploit the insight that exact collision avoidance is not necessary between agents at such large distances, and propose a novel algorithm for extending existing collision avoidance algorithms to perform approximate, long-range collision avoidance. Our formulation performs long-range collision avoidance for distant agent groups to efficiently compute trajectories that are smoother than those obtained with state-of-the-art techniques and at faster rates. Another issue often sidestepped in existing work is that discrete and continuum collision avoidance algorithms have different regions of applicability. For example, low-density crowds cannot be modeled as a continuum, while high-density crowds can be expensive to model using discrete methods. We formulate a hybrid technique for crowd simulation which can accurately and efficiently simulate crowds at any density with seamless transitions between continuum and discrete representations. Our approach blends results from continuum and discrete algorithms, based on local density and velocity variance. In addition to being robust across a variety of group scenarios, it is also highly efficient, running at interactive rates for thousands of agents on portable systems.

  1. Antecedents of approach-avoidance goals in sport.

    PubMed

    Morris, Rebecca L; Kavussanu, Maria

    2008-03-01

    We investigated antecedents of mastery-approach, mastery-avoidance, performance-approach, and performance-avoidance goals in sport. University athletes (n = 230) aged 18-25 years (mean 20.3 years, s = 1.5) completed measures of approach-avoidance goals, perceived parental motivational climate, perceived team motivational climate, perceived competence, and demographics. Multiple regression analyses revealed that mastery-approach goals were positively predicted by perceived competence, mastery team climate, learning/enjoyment parental climate, and gender, with males being higher on these goals than females. Mastery-avoidance goals were predicted positively by learning/enjoyment parental climate and negatively by gender, with females being higher on these goals than males. Performance-approach goals were positively predicted by perceived competence, performance team climate, and gender, with males being higher on these goals than females. Finally, performance-avoidance goals were predicted positively by worry-conducive parental climate. These findings suggest that a mastery or performance climate is related to whether athletes adopt a mastery or performance goal, whereas the perception of competence is associated with whether athletes adopt approach-focused goals. Gender should be considered in future examinations of the approach-avoidance achievement goals in sport.

  2. CircRNA expression pattern and circRNA-miRNA-mRNA network in the pathogenesis of nonalcoholic steatohepatitis

    PubMed Central

    Jin, Xi; Feng, Chun-yan; Xiang, Zun; Chen, Yi-peng; Li, You-ming

    2016-01-01

    The pathogenesis of nonalcoholic steatohepatitis (NASH) is still unclear, where involvement of circRNA is considered for its active role as “miRNA sponge”. Therefore, we aimed to investigate the circRNA expression pattern in NASH and further construct the circRNA-miRNA-mRNA network for in-depth mechanism exploration. Briefly, NASH mice model was established by Methionine and choline deficiency (MCD) diet feeding. Liver circRNA and mRNA profile was initially screened by microarray and ensuing qRT-PCR verification was carried out. The overlapped predicted miRNAs as downstream targets of circRNAs and upstream regulators of mRNAs were verified by qRT-PCR and final circRNA-miRNA-mRNA network was constructed. Gene Ontology (GO) and KEGG pathway analysis were further applied to enrich the huge mRNA microarray data. To sum up, there were 69 up and 63 down regulated circRNAs as well as 2760 up and 2465 down regulated mRNAs in NASH group, comparing with control group. Randomly selected 13 of 14 mRNAs and 2 of 8 circRNAs were successfully verified by qRT-PCR. Through predicted overlapped miRNA verification, four circRNA-miRNA-mRNA pathways were constructed, including circRNA_002581-miR-122-Slc1a5, circRNA_002581- miR-122-Plp2, circRNA_002581-miR-122-Cpeb1 and circRNA_007585-miR-326- UCP2. GO and KEGG pathway analysis also enriched specific mRNAs. Therefore, circRNA profile may serve as candidate for NASH diagnosis and circRNA-miRNA -mRNA pathway may provide novel mechanism for NASH. PMID:27677588

  3. Messenger RNA-based vaccines: progress, challenges, applications.

    PubMed

    Kramps, Thomas; Probst, Jochen

    2013-01-01

    Twenty years after the demonstration that messenger RNA (mRNA) was expressed and immunogenic upon direct injection in mice, the first successful proof-of-concept of specific protection against viral infection in small and large animals was reported. These data indicate wider applicability to infectious disease and should encourage continued translation of mRNA-based prophylactic vaccines into human clinical trials. At the conceptual level, mRNA-based vaccines-more than other genetic vectors-combine the simplicity, safety, and focused immunogenicity of subunit vaccines with favorable immunological properties of live viral vaccines: (1) mRNA vaccines are molecularly defined and carry no excess information. In the environment and upon physical contact, RNA is rapidly degraded by ubiquitous RNases and cannot persist. These characteristics also guarantee tight control over their immunogenic profile (including avoidance of vector-specific immune responses that could interfere with repeated administration), pharmacokinetics, and dosing. (2) mRNA vaccines are synthetically produced by an enzymatic process, just requiring information about the nucleic acid sequence of the desired antigen. This greatly reduces general complications associated with biological vaccine production, such as handling of infectious agents, genetic variability, environmental risks, or restrictions to vaccine distribution. (3) RNA can be tailored to provide potent adjuvant stimuli to the innate immune system by direct activation of RNA-specific receptors; this may reduce the need for additional adjuvants. The formation of native antigen in situ affords great versatility, including intracellular localization, membrane association, posttranslational modification, supra-molecular assembly, or targeted structural optimization of delivered antigen. Messenger RNA vaccines induce balanced immune responses including B cells, helper T cells, and cytotoxic T lymphocytes, rendering them an extremely adaptable

  4. lncRNA/MicroRNA interactions in the vasculature

    PubMed Central

    Ballantyne, MD; McDonald, RA

    2016-01-01

    MicroRNA (miRNA) have gained widespread attention for their role in diverse vascular processes including angiogenesis, apoptosis, proliferation, and migration. Despite great understanding of miRNA expression and function, knowledge of long noncoding RNA (lncRNA) molecular mechanisms still remains limited. The influence of miRNA on lncRNA function, and the converse, is now beginning to emerge. lncRNA may regulate miRNA function by acting as endogenous sponges to regulate gene expression and miRNA have been shown to bind and regulate lncRNA stability. A detailed understanding of the molecular and cellular effects of lncRNA‐miRNA‐mediated interactions in vascular pathophysiology could pave the way for new diagnostic markers and therapeutic approaches, but first there is a requirement for a more detailed understanding of the impact of such regulatory networks. PMID:26910520

  5. Generation of siRNA Nanosheets for Efficient RNA Interference

    NASA Astrophysics Data System (ADS)

    Kim, Hyejin; Lee, Jae Sung; Lee, Jong Bum

    2016-04-01

    After the discovery of small interference RNA (siRNA), nanostructured siRNA delivery systems have been introduced to achieve an efficient regulation of the target gene expression. Here we report a new siRNA-generating two dimensional nanostructure in a formation of nanosized sheet. Inspired by tunable mechanical and functional properties of the previously reported RNA membrane, siRNA nanosized sheets (siRNA-NS) with multiple Dicer cleavage sites were prepared. The siRNA-NS has two dimensional structure, providing a large surface area for Dicer to cleave the siRNA-NS for the generation of functional siRNAs. Furthermore, downregulation of the cellular target gene expression was achieved by delivery of siRNA-NS without chemical modification of RNA strands or conjugation to other substances.

  6. TMV induces RNA decay pathways to modulate gene silencing and disease symptoms.

    PubMed

    Conti, Gabriela; Zavallo, Diego; Venturuzzi, Andrea L; Rodriguez, Maria C; Crespi, Martin; Asurmendi, Sebastian

    2017-01-01

    RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC pathways. In plants, another important RNA degradation mechanism is the post-transcriptional gene silencing (PTGS) mediated by small RNAs (siRNAs). Notably, the RQC pathway antagonizes PTGS by preventing the entry of dysfunctional mRNAs into the silencing pathway to avoid global degradation of mRNA by siRNAs. Viral transcripts must evade RNA degrading mechanisms, thus viruses encode PTGS suppressor proteins to counteract viral RNA silencing. Here, we demonstrate that tobacco plants infected with TMV and transgenic lines expressing TMV MP and CP (coat protein) proteins (which are not linked to the suppression of silencing) display increased transcriptional levels of RNA decay genes. These plants also showed accumulation of cytoplasmic RNA granules with altered structure, increased rates of RNA decay for transgenes and defective transgene PTGS amplification. Furthermore, knockdown of RRP41 or RRP43 RNA exosome components led to lower levels of TMV accumulation with milder symptoms after infection, several developmental defects and miRNA deregulation. Thus, we propose that TMV proteins induce RNA decay pathways (in particular exosome components) to impair antiviral PTGS and this defensive mechanism would constitute an additional counter-defense strategy that lead to disease symptoms.

  7. Approach, avoidance, and affect: a meta-analysis of approach-avoidance tendencies in manual reaction time tasks.

    PubMed

    Phaf, R Hans; Mohr, Sören E; Rotteveel, Mark; Wicherts, Jelte M

    2014-01-01

    Approach action tendencies toward positive stimuli and avoidance tendencies from negative stimuli are widely seen to foster survival. Many studies have shown that approach and avoidance arm movements are facilitated by positive and negative affect, respectively. There is considerable debate whether positively and negatively valenced stimuli prime approach and avoidance movements directly (i.e., immediate, unintentional, implicit, automatic, and stimulus-based), or indirectly (i.e., after conscious or non-conscious interpretation of the situation). The direction and size of these effects were often found to depend on the instructions referring to the stimulus object or the self, and on explicit vs. implicit stimulus evaluation. We present a meta-analysis of 29 studies included for their use of strongly positive and negative stimuli, with 81 effect sizes derived solely from the means and standard deviations (combined N = 1538), to examine the automaticity of the link between affective information processing and approach and avoidance, and to test whether it depends on instruction, type of approach-avoidance task, and stimulus type. Results show a significant small to medium-sized effect after correction for publication bias. The strongest arguments for an indirect link between affect and approach-avoidance were the absence of evidence for an effect with implicit evaluation, and the opposite directions of the effect with self and object-related interpretations. The link appears to be influenced by conscious or non-conscious intentions to deal with affective stimuli.

  8. Approach, avoidance, and affect: a meta-analysis of approach-avoidance tendencies in manual reaction time tasks

    PubMed Central

    Phaf, R. Hans; Mohr, Sören E.; Rotteveel, Mark; Wicherts, Jelte M.

    2014-01-01

    Approach action tendencies toward positive stimuli and avoidance tendencies from negative stimuli are widely seen to foster survival. Many studies have shown that approach and avoidance arm movements are facilitated by positive and negative affect, respectively. There is considerable debate whether positively and negatively valenced stimuli prime approach and avoidance movements directly (i.e., immediate, unintentional, implicit, automatic, and stimulus-based), or indirectly (i.e., after conscious or non-conscious interpretation of the situation). The direction and size of these effects were often found to depend on the instructions referring to the stimulus object or the self, and on explicit vs. implicit stimulus evaluation. We present a meta-analysis of 29 studies included for their use of strongly positive and negative stimuli, with 81 effect sizes derived solely from the means and standard deviations (combined N = 1538), to examine the automaticity of the link between affective information processing and approach and avoidance, and to test whether it depends on instruction, type of approach-avoidance task, and stimulus type. Results show a significant small to medium-sized effect after correction for publication bias. The strongest arguments for an indirect link between affect and approach-avoidance were the absence of evidence for an effect with implicit evaluation, and the opposite directions of the effect with self and object-related interpretations. The link appears to be influenced by conscious or non-conscious intentions to deal with affective stimuli. PMID:24847292

  9. Java Architecture for Detect and Avoid Extensibility and Modeling

    NASA Technical Reports Server (NTRS)

    Santiago, Confesor; Mueller, Eric Richard; Johnson, Marcus A.; Abramson, Michael; Snow, James William

    2015-01-01

    Unmanned aircraft will equip with a detect-and-avoid (DAA) system that enables them to comply with the requirement to "see and avoid" other aircraft, an important layer in the overall set of procedural, strategic and tactical separation methods designed to prevent mid-air collisions. This paper describes a capability called Java Architecture for Detect and Avoid Extensibility and Modeling (JADEM), developed to prototype and help evaluate various DAA technological requirements by providing a flexible and extensible software platform that models all major detect-and-avoid functions. Figure 1 illustrates JADEM's architecture. The surveillance module can be actual equipment on the unmanned aircraft or simulators that model the process by which sensors on-board detect other aircraft and provide track data to the traffic display. The track evaluation function evaluates each detected aircraft and decides whether to provide an alert to the pilot and its severity. Guidance is a combination of intruder track information, alerting, and avoidance/advisory algorithms behind the tools shown on the traffic display to aid the pilot in determining a maneuver to avoid a loss of well clear. All these functions are designed with a common interface and configurable implementation, which is critical in exploring DAA requirements. To date, JADEM has been utilized in three computer simulations of the National Airspace System, three pilot-in-the-loop experiments using a total of 37 professional UAS pilots, and two flight tests using NASA's Predator-B unmanned aircraft, named Ikhana. The data collected has directly informed the quantitative separation standard for "well clear", safety case, requirements development, and the operational environment for the DAA minimum operational performance standards. This work was performed by the Separation Assurance/Sense and Avoid Interoperability team under NASA's UAS Integration in the NAS project.

  10. RNA Whole-Mount In Situ Hybridization Proximity Ligation Assay (rISH-PLA), an Assay for Detecting RNA-Protein Complexes in Intact Cells.

    PubMed

    Roussis, Ioannis M; Myers, Fiona A; Scarlett, Garry P

    2017-03-03

    Techniques for studying RNA-protein interactions have lagged behind those for DNA-protein interactions as a consequence of the complexities associated with working with RNA. This unit describes a method for the adaptation of the In Situ Hybridization-Proximity Ligation Assay (ISH-PLA) to the study of RNA regulation (rISH-PLA). The rISH-PLA assay allows the identification of a given RNA-protein complex at subcellular and single-cell resolution, thus avoiding the lack of spatial resolution and sensitivity associated with assaying heterogeneous cell populations from which conventional RNA-protein interaction detection techniques suffer. This technique will be particularly usefully for studying the activity of RNA binding proteins (RBPs) in complex mixtures of cells, for example tissue sections or whole embryos. © 2017 by John Wiley & Sons, Inc.

  11. RNA-Dependent RNA Polymerase Activity in Influenza Virions

    PubMed Central

    Penhoet, Edward; Miller, Henry; Doyle, Michael; Blatti, Stanley

    1971-01-01

    An RNA-dependent RNA polymerase activity has been detected in purified preparations of influenza virus. In contrast to the replicase activity induced in influenza-infected cells, the virion-associated enzyme has an absolute requirement for Mn++. Most of the RNA synthesized in vitro is complementary to virion RNA. PMID:5288388

  12. Assessment of the RNASound RNA Sampling Card for the Preservation of Influenza Virus RNA

    PubMed Central

    Lau, Hilda; Hurt, Aeron C.

    2016-01-01

    Shipping influenza virus specimens, isolates or purified RNA is normally conducted at ultra-low temperatures using dry ice to ensure minimal degradation of the samples but this is expensive and requires special packaging and shipping conditions. Therefore, alternative methods for shipping influenza viruses or RNA at ambient temperatures would be desirable. The RNASound RNA Sampling Card (FortiusBio LLC, San Diego, CA, USA) is a device that enables specimens or isolates to be applied to a card, whereby viruses are inactivated, while RNA is preserved and purified RNA can also easily be eluted. To evaluate this card, we applied influenza virus cell culture isolate supernatants to either the RNASound card or Whatman Grade No. 1 filter paper (GE Healthcare, Rydalmere, NSW, Australia) and compared the preservation to that of material stored in liquid form. Preservation was tested using influenza A and B viruses at two different storage temperatures [cool (2–8°C) or room temperature (18–22°C)] and these were compared with control material stored at -80°C, for 7, 14, or 28 days. The quality of the RNA recovered was assessed using real time RT-PCR and Sanger sequencing. The RNASound card was effective in preserving influenza RNA at room temperature for up to 28 days, with only a minor change in real-time RT-PCR cycle threshold values for selected gene targets when comparing between viruses applied to the card or stored at -80°C. Similar results were obtained with filter paper, whilst virus in liquid form performed the worst. Nevertheless, as the RNASound card also has the capability to inactivate viruses in addition to preserving RNA at room temperature for many weeks, this makes it feasible to send samples to laboratories using regular mail, and thus avoid the need for expensive shipping conditions requiring biohazard containers and dry ice. Moreover, the quick and simple RNA recovery from the RNASound card allows recipient labs to obtain RNA without the need for

  13. Aminoacyl-RNA synthesis catalyzed by an RNA.

    PubMed

    Illangasekare, M; Sanchez, G; Nickles, T; Yarus, M

    1995-02-03

    An RNA has been selected that rapidly aminoacylates its 2'(3') terminus when provided with phenylalanyl-adenosine monophosphate. That is, the RNA accelerates the same aminoacyl group transfer catalyzed by protein aminoacyl-transfer RNA synthetases. The best characterized RNA reaction requires both Mg2+ and Ca2+. These results confirm a necessary prediction of the RNA world hypothesis and represent efficient RNA reaction (> or = 10(5) times accelerated) at a carbonyl carbon, exemplifying a little explored type of RNA catalysis.

  14. The RNA infrastructure: an introduction to ncRNA networks.

    PubMed

    Collins, Lesley J

    2011-01-01

    The RNA infrastructure connects RNA-based functions. With transcription-to-translation processing forming the core of the network, we can visualise how RNA-based regulation, cleavage and modification are the backbone of cellular function. The key to interpreting the RNA-infrastructure is in understanding how core RNAs (tRNA, mRNA and rRNA) and other ncRNAs operate in a spatial-temporal manner, moving around the nucleus, cytoplasm and organelles during processing, or in response to environmental cues. This chapter summarises the concept of the RNA-infrastructure, and highlights examples of RNA-based networking within prokaryotes and eukaryotes. It describes how transcription-to-translation processes are tightly connected, and explores some similarities and differences between prokaryotic and eukaryotic RNA networking.

  15. Attention Bias of Avoidant Individuals to Attachment Emotion Pictures

    PubMed Central

    Liu, Ying; Ding, Yi; Lu, Luluzi; Chen, Xu

    2017-01-01

    How attachment style affects emotion processing is tightly connected with individuals’ attention bias. This experiment explored avoidant individuals’ attentional engagement and attentional disengagement using a cue-target paradigm in fMRI. The experimental group consisted of 17 avoidant participants, while the control group consisted of 16 secure participants; these were identified by the Experiences in Close Relationships inventory and the Relationship Questionnaire. Each reacted to pictures of positive parent-child attachment, negative parent-child attachment, positive romantic attachment, negative romantic attachment, and neutral non-attachment. Behaviorally, avoidant individuals were slower than secure individuals in responding to emotions and their attentional disengagement effect for negative parent-child emotions was stronger than positive ones. fMRI results showed that avoidant compared to secure individuals activated more strongly in the right superior temporal gyrus, middle occipital gyrus, and the left medial frontal gyrus, middle occipital gyrus, supplementary motor area, and cingulate gyrus. They also showed stronger activation in disengaging from positive than negative emotions in the bilateral fusiform and middle occipital gyri. In conclusion, avoidant individuals could detect emotions as effective as secure individuals in attentioal engaging stages. They can disengage from positive emotions with effective cognitive resources and were harder to get rid of negative emotions with insufficient resource. PMID:28128347

  16. Successive positive contrast in one-way avoidance learning.

    PubMed

    Cándido, Antonio; Maldonado, Antonio; Rodríguez, Alicia; Morales, Alberto

    2002-04-01

    The main finding of these experiments was a positive contrast effect in one-way avoidance learning. Experiment 1 showed that increasing safety time during one-way avoidance training led to improved performance, surpassing that of a control group that had received the high reward (safe time) from the beginning of training. Experiment 2 showed that a similar positive contrast effect occurred when the time spent in the danger compartment before the onset of the warning signal was shortened. These results suggest that time spent in a safe context acts as a reinforcer of the avoidance response; however, its incentive value depends not only on its duration, but also on the length of the time spent in the danger compartment before the onset of the signal. Overall, results also suggest that the avoidance response is a mixture of flight (motivated by fear) and approach (to a safe place) behaviour. The specific weight of the flight or approach component may be a function of the time and the amount of activation of each emotional state (fear or relief) due to opponent homeostatic compensatory processes that occur in the danger and safe compartments during one-way avoidance learning.

  17. All weather collision avoidance for unmanned aircraft systems

    NASA Astrophysics Data System (ADS)

    Contarino, Mark

    2010-04-01

    For decades, military and other national security agencies have been denied unfettered access to the National Air Space (NAS) because their unmanned aircraft lack a highly reliable and effective collision avoidance capability. The controlling agency, the Federal Aviation Administration, justifiably demands "no harm" to the safety of the NAS. To overcome the constraints imposed on Unmanned Aircraft Systems (UAS) use of the NAS, a new, complex, conformable collision avoidance system has been developed - one that will be effective in all flyable weather conditions, overcoming the shortfalls of other sensing systems, including radar, lidar, acoustic, EO/IR, etc., while meeting form factor and cost criteria suitable for Tier II UAS operations. The system also targets Tier I as an ultimate goal, understanding the operational limitations of the smallest UASs may require modification of the design that is suitable for Tier II and higher. The All Weather Sense and Avoid System (AWSAS) takes into account the FAA's plan to incorporate ADS-B (out) for all aircraft by 2020, and it is intended to make collision avoidance capability available for UAS entry into the NAS as early as 2013. When approved, UASs can fly mission or training flights in the NAS free of the constraints presently in place. Upon implementation this system will achieve collision avoidance capability for UASs deployed for national security purposes and will allow expansion of UAS usage for commercial or other civil purposes.

  18. The mnemonic mover: nostalgia regulates avoidance and approach motivation.

    PubMed

    Stephan, Elena; Wildschut, Tim; Sedikides, Constantine; Zhou, Xinyue; He, Wuming; Routledge, Clay; Cheung, Wing-Yee; Vingerhoets, Ad J J M

    2014-06-01

    In light of its role in maintaining psychological equanimity, we proposed that nostalgia--a self-relevant, social, and predominantly positive emotion--regulates avoidance and approach motivation. We advanced a model in which (a) avoidance motivation triggers nostalgia and (b) nostalgia, in turn, increases approach motivation. As a result, nostalgia counteracts the negative impact of avoidance motivation on approach motivation. Five methodologically diverse studies supported this regulatory model. Study 1 used a cross-sectional design and showed that avoidance motivation was positively associated with nostalgia. Nostalgia, in turn, was positively associated with approach motivation. In Study 2, an experimental induction of avoidance motivation increased nostalgia. Nostalgia then predicted increased approach motivation. Studies 3-5 tested the causal effect of nostalgia on approach motivation and behavior. These studies demonstrated that experimental nostalgia inductions strengthened approach motivation (Study 3) and approach behavior as manifested in reduced seating distance (Study 4) and increased helping (Study 5). The findings shed light on nostalgia's role in regulating the human motivation system.

  19. A new collision avoidance model for pedestrian dynamics

    NASA Astrophysics Data System (ADS)

    Wang, Qian-Ling; Chen, Yao; Dong, Hai-Rong; Zhou, Min; Ning, Bin

    2015-03-01

    The pedestrians can only avoid collisions passively under the action of forces during simulations using the social force model, which may lead to unnatural behaviors. This paper proposes an optimization-based model for the avoidance of collisions, where the social repulsive force is removed in favor of a search for the quickest path to destination in the pedestrian’s vision field. In this way, the behaviors of pedestrians are governed by changing their desired walking direction and desired speed. By combining the critical factors of pedestrian movement, such as positions of the exit and obstacles and velocities of the neighbors, the choice of desired velocity has been rendered to a discrete optimization problem. Therefore, it is the self-driven force that leads pedestrians to a free path rather than the repulsive force, which means the pedestrians can actively avoid collisions. The new model is verified by comparing with the fundamental diagram and actual data. The simulation results of individual avoidance trajectories and crowd avoidance behaviors demonstrate the reasonability of the proposed model. Project supported by the National Natural Science Foundation of China (Grant Nos. 61233001 and 61322307) and the Fundamental Research Funds for Central Universities of China (Grant No. 2013JBZ007).

  20. Sun avoidance strategies at the Large Millimeter Telescope

    NASA Astrophysics Data System (ADS)

    Souccar, Kamal; Smith, David R.; Schloerb, F. Peter; Wallace, Gary

    2016-07-01

    The Large Millimeter Telescope observatory is extending its night time operation to the day time. A sun avoidance strategy was therefore implemented in the control system in real-time to avoid excessive heating and damage to the secondary mirror and the prime focus. The LMT uses an "on-the-fly" trajectory generator that receives as input the target location of the telescope and in turn outputs a commanded position to the servo system. The sun avoidance strategy is also implemented "on-the-fly" where it intercepts the input to the trajectory generator and alters that input to avoid the sun. Two sun avoidance strategies were explored. The first strategy uses a potential field approach where the sun is represented as a high-potential obstacle in the telescope's workspace and the target location is represented as a low-potential goal. The potential field is repeatedly calculated as the sun and the telescope move and the telescope follows the induced force by this field. The second strategy is based on path planning using visibility graphs where the sun is represented as a polygonal obstacle and the telescope follows the shortest path from its actual position to the target location via the vertices of the sun's polygon. The visibility graph approach was chosen as the favorable strategy due to the efficiency of its algorithm and the simplicity of its computation.

  1. Disease avoidance as a functional basis for stigmatization

    PubMed Central

    Oaten, Megan; Stevenson, Richard J.; Case, Trevor I.

    2011-01-01

    Stigmatization is characterized by chronic social and physical avoidance of a person(s) by other people. Infectious disease may produce an apparently similar form of isolation—disease avoidance—but on symptom remission this often abates. We propose that many forms of stigmatization reflect the activation of this disease-avoidance system, which is prone to respond to visible signs and labels that connote disease, irrespective of their accuracy. A model of this system is presented, which includes an emotional component, whereby visible disease cues directly activate disgust and contamination, motivating avoidance, and a cognitive component, whereby disease labels bring to mind disease cues, indirectly activating disgust and contamination. The unique predictions of this model are then examined, notably that people who are stigmatized evoke disgust and are contaminating. That animals too show avoidance of diseased conspecifics, and that disease-related stigma targets are avoided in most cultures, also supports this evolutionary account. The more general implications of this approach are then examined, notably how it can be used to good (e.g. improving hygiene) or bad (e.g. racial vilification) ends, by yoking particular labels with cues that connote disease and disgust. This broadening of the model allows for stigmatization of groups with little apparent connection to disease. PMID:22042920

  2. Collision avoidance between two walkers: role-dependent strategies.

    PubMed

    Olivier, Anne-Hélène; Marin, Antoine; Crétual, Armel; Berthoz, Alain; Pettré, Julien

    2013-09-01

    This paper studies strategies for collision avoidance between two persons walking along crossing trajectories. It has been previously demonstrated that walkers are able to anticipate the risk of future collision and to react accordingly. The avoidance task has been described as a mutual control of the future distance of closest approach, MPD (i.e., Mininum Predicted Distance). In this paper, we studied the role of each walker in the task of controlling MPD. A specific question was: does the walker giving way (2nd at the crossing) and the one passing first set similar and coordinated strategies? To answer this question, we inspected the effect of motion adaptations on the future distance of closest approach. This analysis is relevant in the case of collision avoidance because subtle anticipatory behaviors or large last moment adaptations can finally yield the same result upon the final crossing distance. Results showed that collision avoidance is performed collaboratively and the crossing order impacts both the contribution and the strategies used: the participant giving way contributes more than the one passing first to avoid the collision. Both walkers reorient their path but the participant giving way also adapts his speed. Future work is planned to investigate the influence of crossing angle and TTC on adaptations as well as new types of interactions, such as intercepting or meeting tasks.

  3. Experimental characterization of collision avoidance in pedestrian dynamics

    NASA Astrophysics Data System (ADS)

    Parisi, Daniel R.; Negri, Pablo A.; Bruno, Luciana

    2016-08-01

    In the present paper, the avoidance behavior of pedestrians was characterized by controlled experiments. Several conflict situations were studied considering different flow rates and group sizes in crossing and head-on configurations. Pedestrians were recorded from above, and individual two-dimensional trajectories of their displacement were recovered after image processing. Lateral swaying amplitude and step lengths were measured for free pedestrians, obtaining similar values to the ones reported in the literature. Minimum avoidance distances were computed in two-pedestrian experiments. In the case of one pedestrian dodging an arrested one, the avoidance distance did not depend on the relative orientation of the still pedestrian with respect to the direction of motion of the first. When both pedestrians were moving, the avoidance distance in a perpendicular encounter was longer than the one obtained during a head-on approach. It was found that the mean curvature of the trajectories was linearly anticorrelated with the mean speed. Furthermore, two common avoidance maneuvers, stopping and steering, were defined from the analysis of the acceleration and curvature in single trajectories. Interestingly, it was more probable to observe steering events than stopping ones, also the probability of simultaneous steering and stopping occurrences was negligible. The results obtained in this paper can be used to validate and calibrate pedestrian dynamics models.

  4. Animal models of social avoidance and social fear.

    PubMed

    Toth, Iulia; Neumann, Inga D

    2013-10-01

    Social fear and avoidance of social situations represent the main behavioral symptoms of social anxiety disorder (SAD), a highly prevalent anxiety disorder that is poorly elucidated and has rather unsatisfactory therapeutic options. Therefore, animal models are needed to study the underlying etiology and pathophysiology of SAD and to verify the efficacy of possible novel treatment approaches. In this review, we describe and discuss the most important paradigms that have been shown to induce social avoidance and fear in rodents, including foot shock exposure, restraint stress, social isolation, social instability, social defeat, conditioned defeat, social defeat/overcrowding, chronic subordinate colony housing, chronic mild stress, maternal separation and social fear conditioning. We also describe some of the behavioral paradigms used to assess social avoidance and fear in rodents, including the social interaction test, the social preference-avoidance test, the social approach-avoidance test, the three-chambered social approach test, the partition test and the modified Y-maze test. We focus on the behavioral alterations these paradigms induce, especially on social interaction, general anxiety and depressive-like behavior given that SAD is strongly comorbid with anxiety and affective disorders.

  5. Experimental characterization of collision avoidance in pedestrian dynamics.

    PubMed

    Parisi, Daniel R; Negri, Pablo A; Bruno, Luciana

    2016-08-01

    In the present paper, the avoidance behavior of pedestrians was characterized by controlled experiments. Several conflict situations were studied considering different flow rates and group sizes in crossing and head-on configurations. Pedestrians were recorded from above, and individual two-dimensional trajectories of their displacement were recovered after image processing. Lateral swaying amplitude and step lengths were measured for free pedestrians, obtaining similar values to the ones reported in the literature. Minimum avoidance distances were computed in two-pedestrian experiments. In the case of one pedestrian dodging an arrested one, the avoidance distance did not depend on the relative orientation of the still pedestrian with respect to the direction of motion of the first. When both pedestrians were moving, the avoidance distance in a perpendicular encounter was longer than the one obtained during a head-on approach. It was found that the mean curvature of the trajectories was linearly anticorrelated with the mean speed. Furthermore, two common avoidance maneuvers, stopping and steering, were defined from the analysis of the acceleration and curvature in single trajectories. Interestingly, it was more probable to observe steering events than stopping ones, also the probability of simultaneous steering and stopping occurrences was negligible. The results obtained in this paper can be used to validate and calibrate pedestrian dynamics models.

  6. What happens after inbreeding avoidance? Inbreeding by rejected relatives and the inclusive fitness benefit of inbreeding avoidance.

    PubMed

    Duthie, A Bradley; Reid, Jane M

    2015-01-01

    Avoiding inbreeding, and therefore avoiding inbreeding depression in offspring fitness, is widely assumed to be adaptive in systems with biparental reproduction. However, inbreeding can also confer an inclusive fitness benefit stemming from increased relatedness between parents and inbred offspring. Whether or not inbreeding or avoiding inbreeding is adaptive therefore depends on a balance between inbreeding depression and increased parent-offspring relatedness. Existing models of biparental inbreeding predict threshold values of inbreeding depression above which males and females should avoid inbreeding, and predict sexual conflict over inbreeding because these thresholds diverge. However, these models implicitly assume that if a focal individual avoids inbreeding, then both it and its rejected relative will subsequently outbreed. We show that relaxing this assumption of reciprocal outbreeding, and the assumption that focal individuals are themselves outbred, can substantially alter the predicted thresholds for inbreeding avoidance for focal males. Specifically, the magnitude of inbreeding depression below which inbreeding increases a focal male's inclusive fitness increases with increasing depression in the offspring of a focal female and her alternative mate, and it decreases with increasing relatedness between a focal male and a focal female's alternative mate, thereby altering the predicted zone of sexual conflict. Furthermore, a focal male's inclusive fitness gain from avoiding inbreeding is reduced by indirect opportunity costs if his rejected relative breeds with another relative of his. By demonstrating that variation in relatedness and inbreeding can affect intra- and inter-sexual conflict over inbreeding, our models lead to novel predictions for family dynamics. Specifically, parent-offspring conflict over inbreeding might depend on the alternative mates of rejected relatives, and male-male competition over inbreeding might lead to mixed inbreeding

  7. What Happens after Inbreeding Avoidance? Inbreeding by Rejected Relatives and the Inclusive Fitness Benefit of Inbreeding Avoidance

    PubMed Central

    Duthie, A. Bradley; Reid, Jane M.

    2015-01-01

    Avoiding inbreeding, and therefore avoiding inbreeding depression in offspring fitness, is widely assumed to be adaptive in systems with biparental reproduction. However, inbreeding can also confer an inclusive fitness benefit stemming from increased relatedness between parents and inbred offspring. Whether or not inbreeding or avoiding inbreeding is adaptive therefore depends on a balance between inbreeding depression and increased parent-offspring relatedness. Existing models of biparental inbreeding predict threshold values of inbreeding depression above which males and females should avoid inbreeding, and predict sexual conflict over inbreeding because these thresholds diverge. However, these models implicitly assume that if a focal individual avoids inbreeding, then both it and its rejected relative will subsequently outbreed. We show that relaxing this assumption of reciprocal outbreeding, and the assumption that focal individuals are themselves outbred, can substantially alter the predicted thresholds for inbreeding avoidance for focal males. Specifically, the magnitude of inbreeding depression below which inbreeding increases a focal male’s inclusive fitness increases with increasing depression in the offspring of a focal female and her alternative mate, and it decreases with increasing relatedness between a focal male and a focal female’s alternative mate, thereby altering the predicted zone of sexual conflict. Furthermore, a focal male’s inclusive fitness gain from avoiding inbreeding is reduced by indirect opportunity costs if his rejected relative breeds with another relative of his. By demonstrating that variation in relatedness and inbreeding can affect intra- and inter-sexual conflict over inbreeding, our models lead to novel predictions for family dynamics. Specifically, parent-offspring conflict over inbreeding might depend on the alternative mates of rejected relatives, and male-male competition over inbreeding might lead to mixed inbreeding

  8. Biology Today: Respect for RNA.

    ERIC Educational Resources Information Center

    Flannery, Maura C., Ed.

    1991-01-01

    The high points of the story of RNA are presented. The functions of RNA within the cell, how these functions are carried out, and how they evolved are described. The topics of splicing, self-splicing, RNA editing, transcription and translation, and antisense RNA are discussed. (KR)

  9. Sampling out: regulatory avoidance and the Total Coliform Rule.

    PubMed

    Bennear, Lori S; Jessoe, Katrina K; Olmstead, Sheila M

    2009-07-15

    This paper investigates strategic noncompliance with the Total Coliform Rule (TCR) under the U.S. Safe Drinking Water Act. The structure of the TCR provides incentives for some piped drinking water systems to avoid violations by taking additional water quality samples. We estimate the prevalence of this behavior and its potential impact on violations using monthly data for more than 500 Massachusetts water systems, 1993-2003. We find evidence that strategic oversampling is occurring. Water systems most likely to avoid violations by oversampling are most likely to oversample. A significant number of additional violations would have occurred if systems had adhered to legal sampling requirements, rather than oversampling. Our analysis of potential impacts of regulatory avoidance under the current rule suggests that alternative policies for monitoring bacteria in drinking water should be considered.

  10. YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance.

    PubMed

    Müller-Moulé, Patricia; Nozue, Kazunari; Pytlak, Melissa L; Palmer, Christine M; Covington, Michael F; Wallace, Andreah D; Harmer, Stacey L; Maloof, Julin N

    2016-01-01

    Plants respond to neighbor shade by increasing stem and petiole elongation. Shade, sensed by phytochrome photoreceptors, causes stabilization of PHYTOCHROME INTERACTING FACTOR proteins and subsequent induction of YUCCA auxin biosynthetic genes. To investigate the role of YUCCA genes in phytochrome-mediated elongation, we examined auxin signaling kinetics after an end-of-day far-red (EOD-FR) light treatment, and found that an auxin responsive reporter is rapidly induced within 2 hours of far-red exposure. YUCCA2, 5, 8, and 9 are all induced with similar kinetics suggesting that they could act redundantly to control shade-mediated elongation. To test this hypothesis we constructed a yucca2, 5, 8, 9 quadruple mutant and found that the hypocotyl and petiole EOD-FR and shade avoidance responses are completely disrupted. This work shows that YUCCA auxin biosynthetic genes are essential for detectable shade avoidance and that YUCCA genes are important for petiole shade avoidance.

  11. Cognitive factors in panic disorder, agoraphobic avoidance and agoraphobia.

    PubMed

    Berle, David; Starcevic, Vladan; Hannan, Anthony; Milicevic, Denise; Lamplugh, Claire; Fenech, Pauline

    2008-02-01

    There remains a lack of consensus regarding the possibility that especially high levels of panic-related cognitions characterise panic disorder with agoraphobia. We administered the Anxiety Sensitivity Index, the Agoraphobic Cognitions Questionnaire and the Anxious Thoughts and Tendencies Scale as well as measures of agoraphobic avoidance to patients diagnosed with panic disorder with agoraphobia (n=75) and without agoraphobia (n=26). Patients with panic disorder with agoraphobia did not score significantly higher on any of the cognitive variables than did panic disorder patients without agoraphobia. However, most of the cognitive variables showed small to moderate-strength correlations with self-report measures of agoraphobic avoidance. Our findings suggest that anxiety sensitivity, catastrophising of the consequences of panic and a general anxiety-prone cognitive style, although to some extent associated with agoraphobic avoidance, do not discriminate panic disorder with agoraphobia from panic disorder without agoraphobia.

  12. A neuro-collision avoidance strategy for robot manipulators

    NASA Technical Reports Server (NTRS)

    Onema, Joel P.; Maclaunchlan, Robert A.

    1992-01-01

    The area of collision avoidance and path planning in robotics has received much attention in the research community. Our study centers on a combination of an artificial neural network paradigm with a motion planning strategy that insures safe motion of the Articulated Two-Link Arm with Scissor Hand System relative to an object. Whenever an obstacle is encountered, the arm attempts to slide along the obstacle surface, thereby avoiding collision by means of the local tangent strategy and its artificial neural network implementation. This combination compensates the inverse kinematics of a robot manipulator. Simulation results indicate that a neuro-collision avoidance strategy can be achieved by means of a learning local tangent method.

  13. Avoiding loopholes with hybrid Bell-Leggett-Garg inequalities

    NASA Astrophysics Data System (ADS)

    Dressel, Justin; Korotkov, Alexander N.

    2014-01-01

    By combining the postulates of macrorealism with Bell locality, we derive a qualitatively different hybrid inequality that avoids two loopholes that commonly appear in Leggett-Garg and Bell inequalities. First, locally invasive measurements can be used, which avoids the "clumsiness" Leggett-Garg inequality loophole. Second, a single experimental ensemble with fixed analyzer settings is sampled, which avoids the "disjoint sampling" Bell inequality loophole. The derived hybrid inequality has the same form as the Clauser-Horne-Shimony-Holt Bell inequality; however, its quantum violation intriguingly requires weak measurements. A realistic explanation of an observed violation requires either the failure of Bell locality or a preparation conspiracy of finely tuned and nonlocally correlated noise. Modern superconducting and optical systems are poised to implement this test.

  14. Avoiding or restricting defectors in public goods games?

    PubMed Central

    Han, The Anh; Pereira, Luís Moniz; Lenaerts, Tom

    2015-01-01

    When creating a public good, strategies or mechanisms are required to handle defectors. We first show mathematically and numerically that prior agreements with posterior compensations provide a strategic solution that leads to substantial levels of cooperation in the context of public goods games, results that are corroborated by available experimental data. Notwithstanding this success, one cannot, as with other approaches, fully exclude the presence of defectors, raising the question of how they can be dealt with to avoid the demise of the common good. We show that both avoiding creation of the common good, whenever full agreement is not reached, and limiting the benefit that disagreeing defectors can acquire, using costly restriction mechanisms, are relevant choices. Nonetheless, restriction mechanisms are found the more favourable, especially in larger group interactions. Given decreasing restriction costs, introducing restraining measures to cope with public goods free-riding issues is the ultimate advantageous solution for all participants, rather than avoiding its creation. PMID:25540240

  15. Contamination sensitivity and the development of disease-avoidant behaviour

    PubMed Central

    Siegal, Michael; Fadda, Roberta; Overton, Paul G.

    2011-01-01

    Owing to their developing cognitive abilities and their limited knowledge about the biological basis of illness, children often have less expertise at disease avoidance than adults. However, affective reactions to contaminants through the acquisition of disgust and the social and cultural transmissions of knowledge about contamination and contagion provide impetus for children to learn effective disease-avoidant behaviours early in their development. In this article, we review the ontogenetic development of knowledge about contamination and contagion with particular attention to the role of socialization and culture. Together with their emerging cognitive abilities and affective reactions to contaminants, informal and formal cultural learning shape children's knowledge about disease. Through this process, the perceptual cues of contamination are linked to threats of disease outcomes and can act as determinants of disease-avoidant behaviours. PMID:22042919

  16. Incautiously Optimistic: Positively-Valenced Cognitive Avoidance in Adult ADHD

    PubMed Central

    Knouse, Laura E.; Mitchell, John T.

    2015-01-01

    Clinicians who conduct cognitive-behavioral therapy (CBT) targeting attention-deficit/hyperactivity disorder (ADHD) in adulthood have noted that their patients sometimes verbalize overly positive automatic thoughts and set overly optimistic goals. These cognitions are frequently related to failure to engage in compensatory behavioral strategies emphasized in CBT. In this paper, we offer a functional analysis of this problematic pattern, positively-valenced cognitive avoidance, and suggest methods for addressing it within CBT for adult ADHD. We propose that maladaptive positive cognitions function to relieve aversive emotions in the short-term and are therefore negatively reinforced but that, in the long-term, they are associated with decreased likelihood of active coping and increased patterns of behavioral avoidance. Drawing on techniques from Behavioral Activation (BA), we offer a case example to illustrate these concepts and describe step-by-step methods for clinicians to help patients recognize avoidant patterns and engage in more active coping. PMID:25908901

  17. YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance

    PubMed Central

    Müller-Moulé, Patricia; Nozue, Kazunari; Pytlak, Melissa L.; Palmer, Christine M.; Covington, Michael F.; Wallace, Andreah D.; Harmer, Stacey L.

    2016-01-01

    Plants respond to neighbor shade by increasing stem and petiole elongation. Shade, sensed by phytochrome photoreceptors, causes stabilization of PHYTOCHROME INTERACTING FACTOR proteins and subsequent induction of YUCCA auxin biosynthetic genes. To investigate the role of YUCCA genes in phytochrome-mediated elongation, we examined auxin signaling kinetics after an end-of-day far-red (EOD-FR) light treatment, and found that an auxin responsive reporter is rapidly induced within 2 hours of far-red exposure. YUCCA2, 5, 8, and 9 are all induced with similar kinetics suggesting that they could act redundantly to control shade-mediated elongation. To test this hypothesis we constructed a yucca2, 5, 8, 9 quadruple mutant and found that the hypocotyl and petiole EOD-FR and shade avoidance responses are completely disrupted. This work shows that YUCCA auxin biosynthetic genes are essential for detectable shade avoidance and that YUCCA genes are important for petiole shade avoidance. PMID:27761349

  18. Tracking improves performance of biological collision avoidance models.

    PubMed

    Pant, Vivek; Higgins, Charles M

    2012-07-01

    Collision avoidance models derived from the study of insect brains do not perform universally well in practical collision scenarios, although the insects themselves may perform well in similar situations. In this article, we present a detailed simulation analysis of two well-known collision avoidance models and illustrate their limitations. In doing so, we present a novel continuous-time implementation of a neuronally based collision avoidance model. We then show that visual tracking can improve performance of these models by allowing an relative computation of the distance between the obstacle and the observer. We compare the results of simulations of the two models with and without tracking to show how tracking improves the ability of the model to detect an imminent collision. We present an implementation of one of these models processing imagery from a camera to show how it performs in real-world scenarios. These results suggest that insects may track looming objects with their gaze.

  19. Cooperative Exploration and Networking While Preserving Collision Avoidance.

    PubMed

    Kim, Jonghoek

    2016-08-05

    Monitoring of large complex environments, such as underwater environments, is an important task in surveillance. An information (sensor) network can be built to achieve the task. To build an information network in an unknown workspace, we use multiple robots deploying information nodes. While robots build the network, they localize themselves as well as deployed nodes in the global coordinate system. Our multirobot networking strategy is as follows: each robot iteratively visits a frontier, which borders an unsensed area, until all areas are explored. As multiple robots explore the workspace, a robot must avoid colliding with another robot as well as with an obstacle. Hence, we introduce collision avoidance control laws and integrate the control laws with our cooperative networking strategy. Using MATLAB simulations, we verify the scalability and effectiveness of both our networking strategy and the collision avoidance control laws.

  20. Coordinated Dynamic Behaviors for Multirobot Systems With Collision Avoidance.

    PubMed

    Sabattini, Lorenzo; Secchi, Cristian; Fantuzzi, Cesare

    2016-08-16

    In this paper, we propose a novel methodology for achieving complex dynamic behaviors in multirobot systems. In particular, we consider a multirobot system partitioned into two subgroups: 1) dependent and 2) independent robots. Independent robots are utilized as a control input, and their motion is controlled in such a way that the dependent robots solve a tracking problem, that is following arbitrarily defined setpoint trajectories, in a coordinated manner. The control strategy proposed in this paper explicitly addresses the collision avoidance problem, utilizing a null space-based behavioral approach: this leads to combining, in a non conflicting manner, the tracking control law with a collision avoidance strategy. The combination of these control actions allows the robots to execute their task in a safe way. Avoidance of collisions is formally proven in this paper, and the proposed methodology is validated by means of simulations and experiments on real robots.

  1. Avoiding or restricting defectors in public goods games?

    PubMed

    Han, The Anh; Pereira, Luís Moniz; Lenaerts, Tom

    2015-02-06

    When creating a public good, strategies or mechanisms are required to handle defectors. We first show mathematically and numerically that prior agreements with posterior compensations provide a strategic solution that leads to substantial levels of cooperation in the context of public goods games, results that are corroborated by available experimental data. Notwithstanding this success, one cannot, as with other approaches, fully exclude the presence of defectors, raising the question of how they can be dealt with to avoid the demise of the common good. We show that both avoiding creation of the common good, whenever full agreement is not reached, and limiting the benefit that disagreeing defectors can acquire, using costly restriction mechanisms, are relevant choices. Nonetheless, restriction mechanisms are found the more favourable, especially in larger group interactions. Given decreasing restriction costs, introducing restraining measures to cope with public goods free-riding issues is the ultimate advantageous solution for all participants, rather than avoiding its creation.

  2. Increased shelterin mRNA expression in peripheral blood mononuclear cells and skeletal muscle following an ultra-long-distance running event.

    PubMed

    Laye, Matthew J; Solomon, Thomas P J; Karstoft, Kristian; Pedersen, Karin K; Nielsen, Susanne D; Pedersen, Bente K

    2012-03-01

    Located at the end of chromosomes, telomeres are progressively shortened with each replication of DNA during aging. Integral to the regulation of telomere length is a group of proteins making up the shelterin complex, whose tissue-specific function during physiological stress is not well understood. In this study, we examine the mRNA and protein levels of proteins within and associated with the shelterin complex in subjects (n = 8, mean age = 44 yr) who completed a physiological stress of seven marathons in 7 days. Twenty-two to 24 h after the last marathon, subjects had increased mRNA levels of DNA repair enzymes Ku70 and Ku80 (P < 0.05) in both skeletal muscle and peripheral blood mononuclear cells (PBMCs). Additionally, the PBMCs displayed an increment in three shelterin protein mRNA levels (TRF1, TRF2, and Pot-1, P < 0.05) following the event. Seven days of ultrarunning did not result in changes in mean telomere length, telomerase activity, hTert mRNA, or hterc mRNAs found in PBMCs. Higher protein concentrations of TRF2 were found in skeletal muscle vs. PBMCs at rest. Mean telomere length in skeletal muscle did not change and did not contain detectable levels of htert mRNA or telomerase activity. Furthermore, changes in the PBMCs could not be attributed to changes in the proportion of subtypes of CD4(+) or CD8(+) cells. We have provided the first evidence that, in humans, proteins within and associated with the shelterin complex increase at the mRNA level in response to a physiological stress differentially in PBMCs and skeletal muscle.

  3. Stochastic Kinetics of Nascent RNA

    NASA Astrophysics Data System (ADS)

    Xu, Heng; Skinner, Samuel O.; Sokac, Anna Marie; Golding, Ido

    2016-09-01

    The stochastic kinetics of transcription is typically inferred from the distribution of RNA numbers in individual cells. However, cellular RNA reflects additional processes downstream of transcription, hampering this analysis. In contrast, nascent (actively transcribed) RNA closely reflects the kinetics of transcription. We present a theoretical model for the stochastic kinetics of nascent RNA, which we solve to obtain the probability distribution of nascent RNA per gene. The model allows us to evaluate the kinetic parameters of transcription from single-cell measurements of nascent RNA. The model also predicts surprising discontinuities in the distribution of nascent RNA, a feature which we verify experimentally.

  4. The role of aquaporin RWC3 in drought avoidance in rice.

    PubMed

    Lian, Hong-Li; Yu, Xin; Ye, Qin; Ding, Xiaodong; Kitagawa, Yoshichika; Kwak, Sang-Soo; Su, Wei-Ai; Tang, Zhang-Cheng; Ding, Xiao-Song

    2004-04-01

    Although the discovery of aquaporins in plants has resulted in a paradigm shift in the understanding of plant water relations, the relationship between aquaporins and drought resistance still remains elusive. From an agronomic viewpoint, upland rice is traditionally considered as showing drought avoidance. In the investigation of different morphological and physiological responses of upland rice (Oryza sativa L. spp indica cv. Zhonghan 3) and lowland rice (O. sativa L. spp japonica cv. Xiushui 63) to water deficit, we observed young leaf rolling and the remarkable decline of cumulative transpiration in the upland rice. The expression of water channel protein RWC3 mRNA was increased in upland rice at the early response (up to 4 h) to the 20% polyethylene glycol (PEG) 6000 treatment, whereas there was no significant expression changes in lowland rice. Protein levels were increased in upland rice and decreased in lowland rice at 10 h after the water deficit. The up-regulation of RWC3 in upland rice fits well with the knowledge that upland rice adopts the mechanism of drought avoidance. The physiological significance of this RWC3 up-regulation was then explored with the over-expression of RWC3 in transgenic lowland rice (O. sativa L. spp japonica cv. Zhonghua 11) controlled by a stress-inducible SWPA2 promoter. Compared to the wild-type plant, the transgenic lowland rice exhibited higher root osmotic hydraulic conductivity (Lp), leaf water potential and relative cumulative transpiration at the end of 10 h PEG treatment. These results indicated that RWC3 probably played a role in drought avoidance in rice.

  5. Modeling and Simulation of an UAS Collision Avoidance Systems

    NASA Technical Reports Server (NTRS)

    Oliveros, Edgardo V.; Murray, A. Jennifer

    2010-01-01

    This paper describes a Modeling and Simulation of an Unmanned Aircraft Systems (UAS) Collision Avoidance System, capable of representing different types of scenarios for UAS collision avoidance. Commercial and military piloted aircraft currently utilize various systems for collision avoidance such as Traffic Alert and Collision A voidance System (TCAS), Automatic Dependent Surveillance-Broadcast (ADS-B), Radar and ElectroOptical and Infrared Sensors (EO-IR). The integration of information from these systems is done by the pilot in the aircraft to determine the best course of action. In order to operate optimally in the National Airspace System (NAS) UAS have to work in a similar or equivalent manner to a piloted aircraft by applying the principle of "detect-see and avoid" (DSA) to other air traffic. Hence, we have taken these existing sensor technologies into consideration in order to meet the challenge of researching the modeling and simulation of an approximated DSA system. A Schematic Model for a UAS Collision Avoidance System (CAS) has been developed ina closed loop block diagram for that purpose. We have found that the most suitable software to carry out this task is the Satellite Tool Kit (STK) from Analytical Graphics Inc. (AGI). We have used the Aircraft Mission Modeler (AMM) for modeling and simulation of a scenario where a UAS is placed on a possible collision path with an initial intruder and then with a second intruder, but is able to avoid them by executing a right tum maneuver and then climbing. Radars have also been modeled with specific characteristics for the UAS and both intruders. The software provides analytical, graphical user interfaces and data controlling tools which allow the operator to simulate different conditions. Extensive simulations have been carried out which returned excellent results.

  6. Functions of the 5'- and 3'-untranslated regions of tobamovirus RNA.

    PubMed

    Chujo, Tetsuya; Ishibashi, Kazuhiro; Miyashita, Shuhei; Ishikawa, Masayuki

    2015-08-03

    The tobamovirus genome is a 5'-m(7)G-capped RNA that carries a tRNA-like structure at its 3'-terminus. The genomic RNA serves as the template for both translation and negative-strand RNA synthesis. The 5'- and 3'-untranslated regions (UTRs) of the genomic RNA contain elements that enhance translation, and the 3'-UTR also contains the elements necessary for the initiation of negative-strand RNA synthesis. Recent studies using a cell-free viral RNA translation-replication system revealed that a 70-nucleotide region containing a part of the 5'-UTR is bound cotranslationally by tobacco mosaic virus (TMV) replication proteins translated from the genomic RNA and that the binding leads the genomic RNA to RNA replication pathway. This mechanism explains the cis-preferential replication of TMV by the replication proteins. The binding also inhibits further translation to avoid a fatal ribosome-RNA polymerase collision, which might arise if translation and negative-strand synthesis occur simultaneously on a single genomic RNA molecule. Therefore, the 5'- and 3'-UTRs play multiple important roles in the life cycle of tobamovirus.

  7. Peculiar Scaling of Self-Avoiding Walk Contacts

    NASA Astrophysics Data System (ADS)

    Baiesi, Marco; Orlandini, Enzo; Stella, Attilio L.

    2001-08-01

    The nearest neighbor contacts between the two halves of an N-site lattice self-avoiding walk offer an unusual example of scaling random geometry: for N-->∞ they are strictly finite in number but their radius of gyration Rc is power law distributed ~R-τc, where τ>1 is a novel exponent characterizing universal behavior. A continuum of diverging length scales is associated with the Rc distribution. A possibly superuniversal τ = 2 is also expected for the contacts of a self-avoiding or random walk with a confining wall.

  8. Sense and avoid radar for micro/nano robots

    NASA Astrophysics Data System (ADS)

    Molchanov, Pavlo A.; Asmolova, Olha

    2014-10-01

    Revolutionary new fly eye radar sensor technologies based on an array of directional antennas is eliminating the need for a mechanical scanning antenna or complicated phase processor. Proposed sense and avoid radar based on fly eye radar technology can be very small, provides continuous surveillance of entire sky (360 degree by azimuth and elevation) and can be applied for separate or swarm of micro/nano UAS or UGS. Monopulse technology increases bearing accuracy several folds and radar can be multi-functional, multi-frequency. Fly eye micro-radars are inexpensive, can be expendable. Prototype of sense and avoid radar with two directional antennas has been designed and bench tested.

  9. Clear air turbulence avoidance using an airborne microwave radiometer

    NASA Technical Reports Server (NTRS)

    Gary, B. L.

    1984-01-01

    The avoidance of Clear Air Turbulence (CAT) is theoretically possible by selecting flight levels that are a safe distance from the tropopause and inversion layers. These favored sites for CAT generation can be located by an 'airborne microwave radiometer' (AMR) passive sensor system that measures altitude temperature profiles. A flight evaluation of the AMR sensor shows that most CAT could be avoided by following sensor-based advisories. Some limitations still exist for any hypothetical use of the sensor. The principal need is to augment the sensor's 'where' advisories to include useful 'when' forecasts.

  10. Food avoidance in athletes: FODMAP foods on the list.

    PubMed

    Lis, Dana; Ahuja, Kiran D K; Stellingwerff, Trent; Kitic, Cecilia M; Fell, James

    2016-09-01

    We surveyed 910 athletes to assess behaviours towards self-selected food/ingredient avoidance to minimize gastrointestinal distress. Fifty-five percent eliminated at least 1 high fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAP) food/category, with up to 82.6% reporting symptom improvement. In athletes indicating that high FODMAP foods trigger gastrointestinal symptoms, lactose (86.5%) was most frequently eliminated, followed by galactooligosaccharides (23.9%), fructose (23.0%), fructans (6.2%), and polyols (5.4%). Athletes avoid predominantly lactose and to a lesser extent other high FODMAP foods to reduce gastrointestinal distress.

  11. Pyrite footprinting of RNA

    SciTech Connect

    Schlatterer, Joerg C.; Wieder, Matthew S.; Jones, Christopher D.; Pollack, Lois; Brenowitz, Michael

    2012-08-24

    Highlights: Black-Right-Pointing-Pointer RNA structure is mapped by pyrite mediated {sup {center_dot}}OH footprinting. Black-Right-Pointing-Pointer Repetitive experiments can be done in a powdered pyrite filled cartridge. Black-Right-Pointing-Pointer High {sup {center_dot}}OH reactivity of nucleotides imply dynamic role in Diels-Alderase catalysis. -- Abstract: In RNA, function follows form. Mapping the surface of RNA molecules with chemical and enzymatic probes has revealed invaluable information about structure and folding. Hydroxyl radicals ({sup {center_dot}}OH) map the surface of nucleic acids by cutting the backbone where it is accessible to solvent. Recent studies showed that a microfluidic chip containing pyrite (FeS{sub 2}) can produce sufficient {sup {center_dot}}OH to footprint DNA. The 49-nt Diels-Alder RNA enzyme catalyzes the C-C bond formation between a diene and a dienophile. A crystal structure, molecular dynamics simulation and atomic mutagenesis studies suggest that nucleotides of an asymmetric bulge participate in the dynamic architecture of the ribozyme's active center. Of note is that residue U42 directly interacts with the product in the crystallized RNA/product complex. Here, we use powdered pyrite held in a commercially available cartridge to footprint the Diels-Alderase ribozyme with single nucleotide resolution. Residues C39 to U42 are more reactive to {sup {center_dot}}OH than predicted by the solvent accessibility calculated from the crystal structure suggesting that this loop is dynamic in solution. The loop's flexibility may contribute to substrate recruitment and product release. Our implementation of pyrite-mediated {sup {center_dot}}OH footprinting is a readily accessible approach to gleaning information about the architecture of small RNA molecules.

  12. RNA STRAND: The RNA Secondary Structure and Statistical Analysis Database

    PubMed Central

    Andronescu, Mirela; Bereg, Vera; Hoos, Holger H; Condon, Anne

    2008-01-01

    Background The ability to access, search and analyse secondary structures of a large set of known RNA molecules is very important for deriving improved RNA energy models, for evaluating computational predictions of RNA secondary structures and for a better understanding of RNA folding. Currently there is no database that can easily provide these capabilities for almost all RNA molecules with known secondary structures. Results In this paper we describe RNA STRAND – the RNA secondary STRucture and statistical ANalysis Database, a curated database containing known secondary structures of any type and organism. Our new database provides a wide collection of known RNA secondary structures drawn from public databases, searchable and downloadable in a common format. Comprehensive statistical information on the secondary structures in our database is provided using the RNA Secondary Structure Analyser, a new tool we have developed to analyse RNA secondary structures. The information thus obtained is valuable for understanding to which extent and with which probability certain structural motifs can appear. We outline several ways in which the data provided in RNA STRAND can facilitate research on RNA structure, including the improvement of RNA energy models and evaluation of secondary structure prediction programs. In order to keep up-to-date with new RNA secondary structure experiments, we offer the necessary tools to add solved RNA secondary structures to our database and invite researchers to contribute to RNA STRAND. Conclusion RNA STRAND is a carefully assembled database of trusted RNA secondary structures, with easy on-line tools for searching, analyzing and downloading user selected entries, and is publicly available at . PMID:18700982

  13. [Ribosomal RNA Evolution

    NASA Technical Reports Server (NTRS)

    1997-01-01

    It is generally believed that an RNA World existed at an early stage in the history of life. During this early period, RNA molecules are seen to be potentially involved in both catalysis and the storage of genetic information. Translation presents several interrelated themes of inquiry for exobiology. First, it is essential, for understanding the very origin of life, how peptides and eventually proteins might have come to be made on the early Earth in a template directed manner. Second, it is necessary to understand how a machinery of similar complexity to that found in the ribosomes of modern organisms came to exist by the time of the last common ancestor (as detected by 16S rRNA sequence studies). Third, the ribosomal RNAs themselves likely had a very early origin and studies of their history may be very informative about the nature of the RNA World. Moreover, studies of these RNAs will contribute to a better understanding of the potential roles of RNA in early evolution.During the past year we have ave conducted a comparative study of four completely sequenced bacterial genoames. We have focused initially on conservation of gene order. The second component of the project continues to build on the model system for studying the validity of variant 5S rRNA sequences in the vicinity of the modern Vibrio proteolyticus 5S rRNA that we established earlier. This system has made it possible to conduct a detailed and extensive analysis of a local portion of the sequence space. These core methods have been used to construct numerous mutants during the last several years. Although it has been a secondary focus, this work has continued over the last year such that we now have in excess of 125 V. proteolyticus derived constructs which have been made and characterized. We have also continued high resolution NMR work on RNA oligomers originally initiated by G. Kenneth Smith who was funded by a NASA Graduate Student Researcher's Fellowship Award until May of 1996. Mr. Smith

  14. Defining the Collision Avoidance Region for DAA Systems

    NASA Technical Reports Server (NTRS)

    Thipphavong, David; Cone, Andrew; Park, Chunki; Lee, Seung Man; Santiago, Confesor

    2016-01-01

    Unmanned aircraft systems (UAS) will be required to equip with a detect-­-and-­-avoid (DAA) system in order to satisfy the federal aviation regulations to maintain well clear of other aircraft, some of which may be equipped with a Traffic Collision Avoidance System (TCAS) to mitigate the possibility of mid-­-air collisions. As such, the minimum operational performance standards (MOPS) for UAS DAA systems are being designed with TCAS interoperability in mind by a group of industry, government, and academic institutions named RTCA Special Committee-228 (SC-228). This document will discuss the development of the spatial-­-temporal volume known as the collision avoidance region in which the DAA system is not allowed to provide vertical guidance to maintain or regain DAA well clear that could conflict with resolution advisories (RAs) issued by the intruder aircraft's TCAS system. Three collision avoidance region definition candidates were developed based on the existing TCAS RA and DAA alerting definitions. They were evaluated against each other in terms of their interoperability with TCAS RAs and DAA alerts in an unmitigated factorial encounter analysis of 1.3 million simulated pairs.

  15. Secondary Physical Education Avoidance and Gender: Problems and Antidotes

    ERIC Educational Resources Information Center

    Ryan, Thomas; Poirier, Yves

    2012-01-01

    Our goal was to locate and evaluate the barriers that impact and cause females to avoid secondary elective physical education courses. We sought to find answers to stop the further decline of female enrolment in secondary physical education by looking into curricula, program and instructional variables. Anecdotal evidence informed this study which…

  16. Crawling-Onset Age Predicts Visual Cliff Avoidance in Infants.

    ERIC Educational Resources Information Center

    Richards, John E.; Rader, Nancy

    1981-01-01

    Two experiments tested the effects of crawling-onset age, amount of crawling experience, and testing age on avoidance of the deep side of a visual cliff apparatus by human infants. Crawling-onset age disciminated between infants because crawling during the tactile phase interferes with later visual control of locomotion. (Author/RD)

  17. Neural Correlates of Attentional Flexibility during Approach and Avoidance Motivation.

    PubMed

    Calcott, Rebecca D; Berkman, Elliot T

    2015-01-01

    Dynamic, momentary approach or avoidance motivational states have downstream effects on eventual goal success and overall well being, but there is still uncertainty about how those states affect the proximal neurocognitive processes (e.g., attention) that mediate the longer-term effects. Attentional flexibility, or the ability to switch between different attentional foci, is one such neurocognitive process that influences outcomes in the long run. The present study examined how approach and avoidance motivational states affect the neural processes involved in attentional flexibility using fMRI with the aim of determining whether flexibility operates via different neural mechanisms under these different states. Attentional flexibility was operationalized as subjects' ability to switch between global and local stimulus features. In addition to subjects' motivational state, the task context was manipulated by varying the ratio of global to local trials in a block in light of recent findings about the moderating role of context on motivation-related differences in attentional flexibility. The neural processes involved in attentional flexibility differ under approach versus avoidance states. First, differences in the preparatory activity in key brain regions suggested that subjects' preparedness to switch was influenced by motivational state (anterior insula) and the interaction between motivation and context (superior temporal gyrus, inferior parietal lobule). Additionally, we observed motivation-related differences the anterior cingulate cortex during switching. These results provide initial evidence that motivation-induced behavioral changes may arise via different mechanisms in approach versus avoidance motivational states.

  18. 16 CFR 801.90 - Transactions or devices for avoidance.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Transactions or devices for avoidance. 801.90 Section 801.90 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES §...

  19. 16 CFR 801.90 - Transactions or devices for avoidance.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Transactions or devices for avoidance. 801.90 Section 801.90 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES §...

  20. 16 CFR 801.90 - Transactions or devices for avoidance.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Transactions or devices for avoidance. 801.90 Section 801.90 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES §...

  1. 16 CFR 801.90 - Transactions or devices for avoidance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Transactions or devices for avoidance. 801.90 Section 801.90 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES §...

  2. 16 CFR 801.90 - Transactions or devices for avoidance.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Transactions or devices for avoidance. 801.90 Section 801.90 Commercial Practices FEDERAL TRADE COMMISSION RULES, REGULATIONS, STATEMENTS AND INTERPRETATIONS UNDER THE HART-SCOTT-RODINO ANTITRUST IMPROVEMENTS ACT OF 1976 COVERAGE RULES §...

  3. 42 CFR 495.208 - Avoiding duplicate payment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Avoiding duplicate payment. 495.208 Section 495.208 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) STANDARDS AND CERTIFICATION STANDARDS FOR THE ELECTRONIC HEALTH RECORD TECHNOLOGY...

  4. 14 CFR 121.356 - Collision avoidance system.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... maximum certificated takeoff weight (1) An appropriate class of Mode S transponder that meets Technical... meets TSO C-119b (version 7.0), or takeoff weight a later version. (ii) TCAS II that meets TSO C-119a... that meets TSO C-119b (version 7.0), or a later version.(iii) A collision avoidance system...

  5. 14 CFR 129.18 - Collision avoidance system.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) Turbine-powered airplane of more than 33,000 pounds maximum certificated takeoff weight (1) An appropriate... of the followign approved units;(i) TCAS II that meets TSO C-119b (version 7.0), or takeoff weight a...) A collision avoidance system equivalent to TSO C-119b (version 7.0), or a later version, capable...

  6. Implicit Approach-Avoidance Associations for Craved Food Cues

    ERIC Educational Resources Information Center

    Kemps, Eva; Tiggemann, Marika; Martin, Rachel; Elliott, Mecia

    2013-01-01

    Implicit approach associations are well documented for substances such as alcohol, tobacco, and illicit drugs. This study reports two experiments designed to establish and modify such associations specifically in the food craving domain. Experiment 1 used a pictorial implicit association task to examine approach-avoidance associations with…

  7. Avoiding School Management Conflicts and Crisis through Formal Communication

    ERIC Educational Resources Information Center

    Nwogbaga, David M. E.; Nwankwo, Oliver U.; Onwa, Doris O.

    2015-01-01

    This paper examined how conflicts and crisis can be avoided through formal communication. It was necessitated by the observation that most of the conflicts and crisis which tend to mar school management today are functions of the inconsistencies arising from "grapevines, rumours, and gossips" generated through informal communication…

  8. Emotional Avoidance and Panicogenic Responding to a Biological Challenge Procedure

    ERIC Educational Resources Information Center

    Karekla, Maria; Forsyth, John P.; Kelly, Megan M.

    2004-01-01

    Healthy undergraduates high (n = 27) and low (n = 27) in experiential avoidance underwent twelve 20 s inhalations of 20% carbon dioxide-enriched air, while physiological (e.g., skin conductance, heart rate, EMG, and end-tidal CO[subscript 2]) and subjective (e.g., subjective units of distress, evaluative ratings, number and severity of panic…

  9. How Teachers Can Avoid Being Sued: Law and American Education.

    ERIC Educational Resources Information Center

    Greene, Jim

    This paper explores what teachers can do to avoid potential lawsuits. Section 1 describes different types of laws for public and private schools. Section 2 discusses tort liability. Section 3 presents legal principles that apply to educators (in loco parents, intentional torts, strict liability, negligence, foreseeability, assigned duties,…

  10. Enuresis Control through Fading, Escape, and Avoidance Training.

    ERIC Educational Resources Information Center

    Hansen, Gordon D.

    1979-01-01

    A twin signal device that provides both escape and avoidance conditioning in enuresis control was documented with case studies of two enuretic children (eight and nine years old). In addition, a technique of fading as an adjunct to the process was utilized with one subject. (Author/SBH)

  11. Binge Eating and Weight Control: The Role of Experiential Avoidance

    ERIC Educational Resources Information Center

    Lillis, Jason; Hayes, Steven C.; Levin, Michael E.

    2011-01-01

    Two thirds of the adults in the United States are overweight or obese. Binge eating is a barrier to treatment adherence and sustained weight loss, and can be seen as a form of experiential avoidance. The current study analyzed the impact of binge eating on weight reduction in a previously published study of a 1-day acceptance and commitment…

  12. Measuring Avoidance and Inflexibility in Weight Related Problems

    ERIC Educational Resources Information Center

    Lillis, Jason; Hayes, Steven C.

    2008-01-01

    There is growing evidence that experiential avoidance and psychological inflexibility plays a role in a variety of clinical presentations, including health problems. The present study presents preliminary data on a new measure of these processes in relation to difficult weight-related thoughts, feelings, and actions: The Acceptance and Action…

  13. Games Universities Play: And How Donors Can Avoid Them

    ERIC Educational Resources Information Center

    Wooster, Martin Morse

    2011-01-01

    What responsibilities do universities have to donors, many of whom are alumni? Presumably, one responsibility is to respect their wishes when they provide gifts with specific purposes. Yet Martin Morse Wooster shows in this report that universities often neglect the wishes of contributors. "Games Universities Play: And How Donors Can Avoid Them"…

  14. Changes for Avoiding Burnout in Teachers and Advisers

    ERIC Educational Resources Information Center

    Nagy, Mary Lou

    2006-01-01

    All advisers have many concerns and stresses that are unique to their situation. Even though they love their jobs, burnout can creep up without announcing its arrival. Possible signs of burnout may include irritability with students, avoiding responsibility, working harder and getting less done, feeling discouraged and indifferent, showing…

  15. Frequently Asked Questions: IDEA Early Childhood--Disclosure Avoidance

    ERIC Educational Resources Information Center

    Center for IDEA Early Childhood Data Systems (DaSy), 2014

    2014-01-01

    This 2014 document is an adaptation of the 2012 release of "Frequently Asked Questions--Disclosure Avoidance" intended for K-12 audiences. Presented here in the form of responses to frequently asked questions (FAQs) are suggestions intended to provide guidance to IDEA Part C early intervention and Part B 619 preschool special education…

  16. Packet Drop Avoidance for High-speed network transmission protocol

    SciTech Connect

    Jin, Guojun

    2004-05-01

    As network bandwidth continues to grow and longer paths are used to exchange large scientific data between storage systems and GRID computation, it has become increasingly obvious that there is a need to deploy a packet drop avoidance mechanism into network transmission protocols. Current end-to-end congestion avoidance mechanisms used in Transmission Control Protocol (TCP) have worked well on low bandwidth delay product networks, but with newer high-bandwidth delay networks they have shown to be inefficient and prone to unstable. This is largely due to increased network bandwidth coupled with changes in internet traffic patterns. These changes come from a variety of new network applications that are being developed to take advantage of the increased network bandwidth. This paper will examine the end-to-end congestion avoidance mechanism and perform a step-by-step analysis of its theory. In addition we will propose an alternative approach developed as part of a new network transmission protocol. Our alternative protocol uses a packet drop avoidance (PDA) mechanism built on top of the maximum burst size (MBS) theory combined with a real-time available bandwidth algorithm.

  17. Evaluation of Prophylactic Alveolar Trephination: To Avoid Pain.

    DTIC Science & Technology

    1979-06-22

    The purpose of this paper was to evaluate the general use of trephination for avoidance of post-treatment endodontic pain. Also to determine if its...more frequent use would allow more rapid completion of endodontic treatment. Two hundred and twenty-five teeth were filled after one or two

  18. 14 CFR 437.65 - Collision avoidance analysis.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Collision avoidance analysis. 437.65 Section 437.65 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.65 Collision...

  19. 14 CFR 437.65 - Collision avoidance analysis.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false Collision avoidance analysis. 437.65 Section 437.65 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.65 Collision...

  20. 14 CFR 437.65 - Collision avoidance analysis.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false Collision avoidance analysis. 437.65 Section 437.65 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.65 Collision...

  1. 14 CFR 437.65 - Collision avoidance analysis.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Collision avoidance analysis. 437.65 Section 437.65 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.65 Collision...

  2. 14 CFR 437.65 - Collision avoidance analysis.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Collision avoidance analysis. 437.65 Section 437.65 Aeronautics and Space COMMERCIAL SPACE TRANSPORTATION, FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION LICENSING EXPERIMENTAL PERMITS Safety Requirements § 437.65 Collision...

  3. 47 CFR 51.609 - Determination of avoided retail costs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... (customer services) (§§ 32.6611, 32.6613, 32.6621, 32.6622, and 32.6623 of this chapter); (2) Include, as... Section 51.609 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES... reasonably can be avoided when an incumbent LEC provides a telecommunications service for resale at...

  4. Indigenous Knowledge in the Science Curriculum: Avoiding Neo-Colonialism

    ERIC Educational Resources Information Center

    Ryan, Ann

    2008-01-01

    Science education in Papua New Guinea has been influenced by neo-colonial practices that have significantly contributed to the silencing of the Papua New Guinea voice. This silencing has led to the production of science curriculum documents that are irrelevant to the students for whom they are written. To avoid being caught up in neo-colonial…

  5. Listening for avoidance: narrative form and defensiveness in adolescent memories.

    PubMed

    Nelson, Kristin L; Bein, Edward; Huemer, Julia; Ryst, Erika; Steiner, Hans

    2009-12-01

    We describe a linguistic clue to speakers' states of mind that has utility for psychotherapists and counselors, and summarize the theoretical and empirical support for using this clue in clinical practice. Specifically, we posit that the degree to which people relate stressful episodes from their lives as a chronological sequence of events is negatively associated with the extent to which they self-protectively avoid experiencing negative affect. We review relevant discussions and findings from linguistics and psychology, and then present a new study that replicates previous research. In this study of the relationship between defensive avoidance and the narrative structure of stressful memories in non-clinical adolescents, 168 high school students spoke for 10 min into a tape recorder about "your most stressful life event." Transcribed interviews were analyzed for narrative immersion, the extent to which the past is retold in chronological order, using a method adopted from Labov and Waletzky. A negative association was found between narrative immersion and avoidance (as operationalized by scores on the Marlowe Crowne Social Desirability Scale). Listening for narrative immersion in the speech of clients discussing past stressful times may therefore represent a useful tool in exploring defensive avoidance of stressful episodic memories.

  6. Airborne Collision Avoidance Systems and Air Traffic Management Safety

    NASA Astrophysics Data System (ADS)

    Brooker, Peter

    2005-01-01

    A new ICAO Policy on Airborne Collision Avoidance Systems is needed, which recognizes it to be an integrated part of the air traffic management system's safety defences; and that should be fully included in hazard analyses for the total system's design safety targets.

  7. Maternal Adjustment Following Preterm Birth: Contributions of Experiential Avoidance

    ERIC Educational Resources Information Center

    Greco, Laurie A.; Heffner, Michelle; Poe, Susannah; Ritchie, Susan; Polak, Mark; Lynch, Susan K.

    2005-01-01

    The birth of a preterm infant has been linked with parental distress and adjustment difficulties, yet little is known about the psychosocial factors contributing to this association. Using a cross-sectional design, we therefore examined maternal adjustment following preterm birth, with an emphasis on the potential role of experiential avoidance.…

  8. Information Seeking and Avoidance Behavior in School Library Distance Learning

    ERIC Educational Resources Information Center

    Du, Yunfei

    2010-01-01

    Library science students in school librarianship were surveyed to determine their information seeking and avoidance behaviors in Web-based online environments. Two coping styles were identified among students. Barriers to student online collaboration, such as individual preferences, concerns on efficiency, and lack of mutual trust, were observed.…

  9. Kin encounter rate and inbreeding avoidance in canids

    USGS Publications Warehouse

    Geffen, Eli; Kam, Michael; Hefner, Reuven; Hersteinsson, Pall; Angerbjorn, Anders; Dalen, Love; Fuglei, Eva; Noren, Karin; Adams, Jennifer R.; Vicetich, John; Meier, Thomas J.; Mech, L.D.; VonHoldt, Bridgett M.; Stahler, Daniel R.; Wayne, Robert K.

    2011-01-01

    Mating with close kin can lead to inbreeding depression through the expression of recessive deleterious alleles and loss of heterozygosity. Mate selection may be affected by kin encounter rate, and inbreeding avoidance may not be uniform but associated with age and social system. Specifically, selection for kin recognition and inbreeding avoidance may be more developed in species that live in family groups or breed cooperatively. To test this hypothesis, we compared kin encounter rate and the proportion of related breeding pairs in noninbred and highly inbred canid populations. The chance of randomly encountering a full sib ranged between 1–8% and 20–22% in noninbred and inbred canid populations, respectively. We show that regardless of encounter rate, outside natal groups mates were selected independent of relatedness. Within natal groups, there was a significant avoidance of mating with a relative. Lack of discrimination against mating with close relatives outside packs suggests that the rate of inbreeding in canids is related to the proximity of close relatives, which could explain the high degree of inbreeding depression observed in some populations. The idea that kin encounter rate and social organization can explain the lack of inbreeding avoidance in some species is intriguing and may have implications for the management of populations at risk.

  10. Kin encounter rate and inbreeding avoidance in canids

    USGS Publications Warehouse

    Geffen, E.; Kam, M.; Hefner, R.; Hersteinsson, P.; Angerbjorn, A.; Dalen, L.; Fuglei, E.; Noren, K.; Adams, J.R.; Vucetich, J.; Meier, T.J.; Mech, L.D.; Vonholdt, B.M.; Stahler, D.R.; Wayne, R.K.

    2011-01-01

    Mating with close kin can lead to inbreeding depression through the expression of recessive deleterious alleles and loss of heterozygosity. Mate selection may be affected by kin encounter rate, and inbreeding avoidance may not be uniform but associated with age and social system. Specifically, selection for kin recognition and inbreeding avoidance may be more developed in species that live in family groups or breed cooperatively. To test this hypothesis, we compared kin encounter rate and the proportion of related breeding pairs in noninbred and highly inbred canid populations. The chance of randomly encountering a full sib ranged between 1-8% and 20-22% in noninbred and inbred canid populations, respectively. We show that regardless of encounter rate, outside natal groups mates were selected independent of relatedness. Within natal groups, there was a significant avoidance of mating with a relative. Lack of discrimination against mating with close relatives outside packs suggests that the rate of inbreeding in canids is related to the proximity of close relatives, which could explain the high degree of inbreeding depression observed in some populations. The idea that kin encounter rate and social organization can explain the lack of inbreeding avoidance in some species is intriguing and may have implications for the management of populations at risk. ?? 2011 Blackwell Publishing Ltd.

  11. School Bullying: Tools for Avoiding Harm and Liability

    ERIC Educational Resources Information Center

    McGrath, Mary Jo

    2006-01-01

    Every hour of every day, students experience bullying and harassment at school by their peers. The immediate and long-term impact on the victims' learning capabilities, emotional health, and self-esteem is staggering. " School Bullying: Tools for Avoiding Harm and Liability" tackles this critical problem with an easy-to-use framework that guides…

  12. The "Eye Avoidance" Hypothesis of Autism Face Processing.

    PubMed

    Tanaka, James W; Sung, Andrew

    2016-05-01

    Although a growing body of research indicates that children with autism spectrum disorder (ASD) exhibit selective deficits in their ability to recognize facial identities and expressions, the source of their face impairment is, as yet, undetermined. In this paper, we consider three possible accounts of the autism face deficit: (1) the holistic hypothesis, (2) the local perceptual bias hypothesis and (3) the eye avoidance hypothesis. A review of the literature indicates that contrary to the holistic hypothesis, there is little evidence to suggest that individuals with autism do perceive faces holistically. The local perceptual bias account also fails to explain the selective advantage that ASD individuals demonstrate for objects and their selective disadvantage for faces. The eye avoidance hypothesis provides a plausible explanation of face recognition deficits where individuals with ASD avoid the eye region because it is perceived as socially threatening. Direct eye contact elicits a increased physiological response as indicated by heightened skin conductance and amygdala activity. For individuals with autism, avoiding the eyes is an adaptive strategy, however, this approach interferes with the ability to process facial cues of identity, expressions and intentions, exacerbating the social challenges for persons with ASD.

  13. 47 CFR 15.711 - Interference avoidance mechanisms.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... auxiliary service links; private land mobile service/commercial radio service (PLMRS/CMRS) operations... 47 Telecommunication 1 2010-10-01 2010-10-01 false Interference avoidance mechanisms. 15.711 Section 15.711 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL RADIO FREQUENCY...

  14. Sensorimotor Model of Obstacle Avoidance in Echolocating Bats

    PubMed Central

    Vanderelst, Dieter; Holderied, Marc W.; Peremans, Herbert

    2015-01-01

    Bat echolocation is an ability consisting of many subtasks such as navigation, prey detection and object recognition. Understanding the echolocation capabilities of bats comes down to isolating the minimal set of acoustic cues needed to complete each task. For some tasks, the minimal cues have already been identified. However, while a number of possible cues have been suggested, little is known about the minimal cues supporting obstacle avoidance in echolocating bats. In this paper, we propose that the Interaural Intensity Difference (IID) and travel time of the first millisecond of the echo train are sufficient cues for obstacle avoidance. We describe a simple control algorithm based on the use of these cues in combination with alternating ear positions modeled after the constant frequency bat Rhinolophus rouxii. Using spatial simulations (2D and 3D), we show that simple phonotaxis can steer a bat clear from obstacles without performing a reconstruction of the 3D layout of the scene. As such, this paper presents the first computationally explicit explanation for obstacle avoidance validated in complex simulated environments. Based on additional simulations modelling the FM bat Phyllostomus discolor, we conjecture that the proposed cues can be exploited by constant frequency (CF) bats and frequency modulated (FM) bats alike. We hypothesize that using a low level yet robust cue for obstacle avoidance allows bats to comply with the hard real-time constraints of this basic behaviour. PMID:26502063

  15. Large angle slew maneuvers with autonomous sun vector avoidance

    NASA Astrophysics Data System (ADS)

    McInnes, Colin R.

    1994-07-01

    The paper presents a methodology developed which allows large angle slew maneuvers to be achieved with autonomous avoidance of the sun vector or other undesired orientations. It has been demonstrated that the local potential shape may be manipulated to guide the satellite attitude during the slew. This guidance allows a globally stable, point to point constrained maneuver using analytic guidance commands.

  16. Concept development of automatic guidance for rotorcraft obstacle avoidance

    NASA Technical Reports Server (NTRS)

    Cheng, Victor H. L.

    1990-01-01

    The automatic guidance of rotorcraft for obstacle avoidance in nap-of-the-earth flight is studied. A hierarchical breakdown of the guidance components is used to identify the functional requirements. These requirements and anticipated sensor capabilities lead to a preliminary guidance concept, which has been evaluated via computer simulations.

  17. Avoiding Counselor Malpractice. ACA Legal Series, Volume 12.

    ERIC Educational Resources Information Center

    Crawford, Robert L.

    The possibility of lawsuits is a general condition of citizenship; potential lawsuits based on allegations of malpractice is a routine part of professional practice. This monograph provides a guide for taking some reasonable precautions to avoid malpractice suits. The text provides an outline that counselors may use in developing a legal and…

  18. [Hypersynchronous EEG activity and conditioned avoidance reflexes in rats].

    PubMed

    Frenzel, C; Kästner, I; Müller, M

    1978-01-01

    The influence of dimethylsulfolane on active avoidance conditioning with simultaneous registration of EEG was investigated. Dimethylsulfolane, which shows some pharmacological properties of pentylenetetrazole causes a decrease of the amount of conditioned reactions. There was no strong correlation between the impairment of learning ability and the number of EEG spindle discharges after dimethylsulfolane.

  19. Differential Cost Avoidance and Successful Criminal Careers: Random or Rational?

    ERIC Educational Resources Information Center

    Kazemian, Lila; Le Blanc, Marc

    2007-01-01

    Using a sample of adjudicated French Canadian males from the Montreal Two Samples Longitudinal Study, this article investigates individual and social characteristics associated with differential cost avoidance. The main objective of this study is to determine whether such traits are randomly distributed across differential degrees of cost…

  20. 40 CFR 141.171 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false Criteria for avoiding filtration. 141.171 Section 141.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and...

  1. 40 CFR 141.71 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.71 Criteria for avoiding filtration. A public water system that uses a surface water source must meet all of...)(C)(iii), that filtration is required. A public water system that uses a ground water source...

  2. 40 CFR 141.71 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.71 Criteria for avoiding filtration. A public water system that uses a surface water source must meet all of...)(C)(iii), that filtration is required. A public water system that uses a ground water source...

  3. 40 CFR 141.171 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false Criteria for avoiding filtration. 141.171 Section 141.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and...

  4. 40 CFR 141.171 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 24 2012-07-01 2012-07-01 false Criteria for avoiding filtration. 141.171 Section 141.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and...

  5. 40 CFR 141.71 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.71 Criteria for avoiding filtration. A public water system that uses a surface water source must meet all of...)(C)(iii), that filtration is required. A public water system that uses a ground water source...

  6. 40 CFR 141.71 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.71 Criteria for avoiding filtration. A public water system that uses a surface water source must meet all of...)(C)(iii), that filtration is required. A public water system that uses a ground water source...

  7. 40 CFR 141.71 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.71 Criteria for avoiding filtration. A public water system that uses a surface water source must meet all of...)(C)(iii), that filtration is required. A public water system that uses a ground water source...

  8. 40 CFR 141.171 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Criteria for avoiding filtration. 141.171 Section 141.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and...

  9. 40 CFR 141.171 - Criteria for avoiding filtration.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Criteria for avoiding filtration. 141.171 Section 141.171 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and...

  10. Acquisition, extinction and temporal discrimination in human conditioned avoidance.

    PubMed

    Molet, Mikaël; Leconte, Claire; Rosas, Juan M

    2006-09-01

    Two experiments were conducted with the aim of designing a videogame for the study of human conditioned avoidance. Participants had to destroy enemy spaceships with the goal of increasing the score in a counter. Coloured signals might announce the launching of a bomb that could hit participant's spaceship producing a 30 points decrease in participant's score. Three groups of participants were trained in discriminating between a warning signal (W) and a safety signal (S) in Experiment 1. Instrumental group could avoid the loss of points by hiding the spaceship before the offset of W. Participants in the Yoked group received the same treatment received by their instrumental partners, regardless of their behaviour. In the Pavlovian group, W was always followed by the loss of points, regardless of participant's behaviour. Discrimination between W and S was better in the Instrumental groups than in the Yoked and Pavlovian control groups. Experiment 2 found extinction of avoidance when the warning signal was not followed by the bomb. Temporal discrimination was found within the participants that received the instrumental contingency in both experiments, with higher avoidance response towards the end of the warning signal. Temporal discrimination disappeared after extinction in Experiment 2.

  11. Helping Teenage Girls Avoid the Female Athlete Triad.

    ERIC Educational Resources Information Center

    Ilardi, Deb

    2002-01-01

    Describes how school nurses can advocate for adolescent female students and help them avoid the female athlete triad that includes disordered eating, amenorrhea, and osteoporosis. The article focuses on consequences of the triad, how to uncover the symptoms, working to improve public support, and creating a successful program through partnership.…

  12. More Misconceptions to Avoid When Teaching about Plants

    ERIC Educational Resources Information Center

    Hershey, David R.

    2005-01-01

    As follow-up to a previous article "Avoid Misconceptions When Teaching about Plants," the author identifies fifty additional misconceptions. Undergeneralizations are added to the list of oversimplifications, obsolete concepts, terms, misidentifications, and flawed research. A glossary at the end of the article compares words used in botany with…

  13. 78 FR 35262 - Detection and Avoidance of Counterfeit Electronic Parts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Defense Acquisition Regulations System Detection and Avoidance of Counterfeit Electronic Parts AGENCY...: DoD is hosting a public meeting to obtain the views of experts and interested parties in...

  14. Precision Landing and Hazard Avoidance (PL&HA) Domain

    NASA Technical Reports Server (NTRS)

    Robertson, Edward A.; Carson, John M., III

    2016-01-01

    The Precision Landing and Hazard Avoidance (PL&HA) domain addresses the development, integration, testing, and spaceflight infusion of sensing, processing, and GN&C (Guidance, Navigation and Control) functions critical to the success and safety of future human and robotic exploration missions. PL&HA sensors also have applications to other mission events, such as rendezvous and docking.

  15. Accident Avoidance Skill Training and Performance Testing. Final Report.

    ERIC Educational Resources Information Center

    Hatterick, G. Richard; Barthurst, James R.

    A two-phased study was conducted to determine the feasibility of training drivers to acquire skills needed to avoid critical conflict motor vehicle accidents, and to develop the procedures and materials necessary for such training. Basic data were derived from indepth accident investigations and task analyses of driver behavior. Principal…

  16. Tibialis anterior tenosynovitis: Avoiding extensor retinaculum damage during endoscopic debridement.

    PubMed

    Maquirriain, Javier; Sammartino, Martín; Ghisi, Juan P; Mazzuco, Juan

    2003-02-01

    Tibialis anterior tenosynovitis is a rare orthopaedic condition that usually resolves with conservative treatment. Surgery may be required for chronic cases and endoscopy seems to be a valid therapeutic alternative. During debridement of the hypertrophic synovium, care must be taken to avoid damaging the extensor retinaculum to prevent potential postoperative bowstring phenomenon of the tendon.

  17. All About PID - Testing and Avoidance in the Field

    SciTech Connect

    Hacke, Peter; Johnston, Steve

    2016-09-01

    Potential-induced degradation can cause significant power loss in modules if the appropriate precautions are not taken. In the first part of a new series in PV Tech Power on module failure, Peter Hacke and Steve Johnston assess the current state-of-the-art in detecting, avoiding and mitigating the worst effects of PID.

  18. 47 CFR 74.803 - Frequency selection to avoid interference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Power Auxiliary Stations § 74.803 Frequency selection to avoid interference. (a) Where two or more low.... In these bands, low power auxiliary station usage is secondary to TV broadcasting and land mobile... occurs, low power auxiliary station operation must immediately cease and may not be resumed until...

  19. 47 CFR 74.803 - Frequency selection to avoid interference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Power Auxiliary Stations § 74.803 Frequency selection to avoid interference. (a) Where two or more low.... In these bands, low power auxiliary station usage is secondary to TV broadcasting and land mobile... occurs, low power auxiliary station operation must immediately cease and may not be resumed until...

  20. 47 CFR 74.803 - Frequency selection to avoid interference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Power Auxiliary Stations § 74.803 Frequency selection to avoid interference. (a) Where two or more low.... In these bands, low power auxiliary station usage is secondary to TV broadcasting and land mobile... occurs, low power auxiliary station operation must immediately cease and may not be resumed until...

  1. 47 CFR 74.803 - Frequency selection to avoid interference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Power Auxiliary Stations § 74.803 Frequency selection to avoid interference. (a) Where two or more low.... In these bands, low power auxiliary station usage is secondary to TV broadcasting and land mobile... occurs, low power auxiliary station operation must immediately cease and may not be resumed until...

  2. 50 CFR 600.510 - Gear avoidance and disposal.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Gear avoidance and disposal. 600.510 Section 600.510 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS Foreign Fishing §...

  3. 50 CFR 600.510 - Gear avoidance and disposal.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Gear avoidance and disposal. 600.510 Section 600.510 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS Foreign Fishing §...

  4. Behavioural accident avoidance science: understanding response in collision incipient conditions.

    PubMed

    Hancock, P A; de Ridder, S N

    2003-10-10

    Road traffic accidents are the single greatest cause of fatality in the workplace and the primary cause of all accidental death in the US to the age of 78. However, behavioural analysis of response in the final seconds and milliseconds before collision has been a most difficult proposition since the quantitative recording of such events has largely been beyond cost feasibility for road transportation. Here, a new and innovative research strategy is reported that permits just such a form of investigation to be conducted in a safe and effective manner. Specifically, a linked simulation environment has been constructed in which drivers are physically located in two adjacent, full-vehicle simulators acting within a shared single virtual driving world. As reported here for the first time, this innovative technology creates situations that provide avoidance responses paralleling those observed in real-world conditions. Within this shared virtual world 46 participants (25 female, 21 male) were tested who met in two ambiguous traffic situations: an intersection and a hill scenario. At the intersection the two drivers approached each other at an angle of 135 degrees and buildings placed at the intersection blocked the view of both drivers from early detection of the opposing vehicle. The second condition represented a 'wrong' way conflict. Each driver proceeded along a three-lane highway from opposite directions. A hill impeded the oncoming view of each driver who only saw the conflicting vehicle briefly as it crested the brow of the hill. Driver avoidance responses of steering wheel, brake, and accelerator activation were recorded to the nearest millisecond. Qualitative results were obtained through a post-experience questionnaire in which participants were asked about their driving habits, simulator experience and their particular response to the experimental events which they had encountered. The results indicated that: (1) situations have been created which provided

  5. Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription

    NASA Astrophysics Data System (ADS)

    Jang, Mihue; Kim, Jong Hwan; Nam, Hae Yun; Kwon, Ick Chan; Ahn, Hyung Jun

    2015-08-01

    For therapeutic applications of siRNA, there are technical challenges with respect to targeted and systemic delivery. We here report a new siRNA carrier, RNAtr NPs, in a way that multiple tandem copies of RNA hairpins as a result of rolling circle transcription (RCT) can be readily adapted in tumour-targeted and systemic siRNA delivery. RNAtr NPs provide a means of condensing large amounts of multimeric RNA transcripts into the compact nanoparticles, especially without the aid of polycationic agents, and thus reduce the risk of immunogenicity and cytotoxicity by avoiding the use of synthetic polycationic reagents. This strategy allows the design of a platform technology for systemic delivery of siRNA to tumour sites, because RCT reaction, which enzymatically generates RNA polymers in multiple copy numbers at low cost, can lead to directly accessible routes to targeted and systemic delivery. Therefore, RNAtr NPs suggest great potentials as the siRNA therapeutics for cancer treatment.

  6. Shaping tRNA

    ERIC Educational Resources Information Center

    Priano, Christine

    2013-01-01

    This model-building activity provides a quick, visual, hands-on tool that allows students to examine more carefully the cloverleaf structure of a typical tRNA molecule. When used as a supplement to lessons that involve gene expression, this exercise reinforces several concepts in molecular genetics, including nucleotide base-pairing rules, the…

  7. RNase non-sensitive and endocytosis independent siRNA delivery system: delivery of siRNA into tumor cells and high efficiency induction of apoptosis

    NASA Astrophysics Data System (ADS)

    Jiang, Xinglu; Wang, Guobao; Liu, Ru; Wang, Yaling; Wang, Yongkui; Qiu, Xiaozhong; Gao, Xueyun

    2013-07-01

    To date, RNase degradation and endosome/lysosome trapping are still serious problems for siRNA-based molecular therapy, although different kinds of delivery formulations have been tried. In this report, a cell penetrating peptide (CPP, including a positively charged segment, a linear segment, and a hydrophobic segment) and a single wall carbon nanotube (SWCNT) are applied together by a simple method to act as a siRNA delivery system. The siRNAs first form a complex with the positively charged segment of CPP via electrostatic forces, and the siRNA-CPP further coats the surface of the SWCNT via hydrophobic interactions. This siRNA delivery system is non-sensitive to RNase and can avoid endosome/lysosome trapping in vitro. When this siRNA delivery system is studied in Hela cells, siRNA uptake was observed in 98% Hela cells, and over 70% mRNA of mammalian target of rapamycin (mTOR) is knocked down, triggering cell apoptosis on a significant scale. Our siRNA delivery system is easy to handle and benign to cultured cells, providing a very efficient approach for the delivery of siRNA into the cell cytosol and cleaving the target mRNA therein.

  8. The RNA Worlds in Context

    PubMed Central

    Cech, Thomas R.

    2012-01-01

    There are two RNA worlds. The first is the primordial RNA world, a hypothetical era when RNA served as both information and function, both genotype and phenotype. The second RNA world is that of today's biological systems, where RNA plays active roles in catalyzing biochemical reactions, in translating mRNA into proteins, in regulating gene expression, and in the constant battle between infectious agents trying to subvert host defense systems and host cells protecting themselves from infection. This second RNA world is not at all hypothetical, and although we do not have all the answers about how it works, we have the tools to continue our interrogation of this world and refine our understanding. The fun comes when we try to use our secure knowledge of the modern RNA world to infer what the primordial RNA world might have looked like. PMID:21441585

  9. Mapping RNA-RNA interactome and RNA structure in vivo by MARIO.

    PubMed

    Nguyen, Tri C; Cao, Xiaoyi; Yu, Pengfei; Xiao, Shu; Lu, Jia; Biase, Fernando H; Sridhar, Bharat; Huang, Norman; Zhang, Kang; Zhong, Sheng

    2016-06-24

    The pervasive transcription of our genome presents a possibility of revealing new genomic functions by investigating RNA interactions. Current methods for mapping RNA-RNA interactions have to rely on an 'anchor' protein or RNA and often require molecular perturbations. Here we present the MARIO (Mapping RNA interactome in vivo) technology to massively reveal RNA-RNA interactions from unperturbed cells. We mapped tens of thousands of endogenous RNA-RNA interactions from mouse embryonic stem cells and brain. We validated seven interactions by RNA antisense purification and one interaction using single-molecule RNA-FISH. The experimentally derived RNA interactome is a scale-free network, which is not expected from currently perceived promiscuity in RNA-RNA interactions. Base pairing is observed at the interacting regions between long RNAs, including transposon transcripts, suggesting a class of regulatory sequences acting in trans. In addition, MARIO data reveal thousands of intra-molecule interactions, providing in vivo data on high-order RNA structures.

  10. Environmental triggers and avoidance in the management of asthma

    PubMed Central

    Gautier, Clarisse; Charpin, Denis

    2017-01-01

    Identifying asthma triggers forms the basis of environmental secondary prevention. These triggers may be allergenic or nonallergenic. Allergenic triggers include indoor allergens, such as house dust mites (HDMs), molds, pets, cockroaches, and rodents, and outdoor allergens, such as pollens and molds. Clinical observations provide support for the role of HDM exposure as a trigger, although avoidance studies provide conflicting results. Molds and their metabolic products are now considered to be triggers of asthma attacks. Pets, dogs, and especially cats can undoubtedly trigger asthmatic symptoms in sensitized subjects. Avoidance is difficult and rarely adhered to by families. Cockroach allergens contribute to asthma morbidity, and avoidance strategies can lead to clinical benefit. Mouse allergens are mostly found in inner-city dwellings, but their implication in asthma morbidity is debated. In the outdoors, pollens can induce seasonal asthma in sensitized individuals. Avoidance relies on preventing pollens from getting into the house and on minimizing seasonal outdoor exposure. Outdoor molds may lead to severe asthma exacerbations. Nonallergenic triggers include viral infections, active and passive smoking, meteorological changes, occupational exposures, and other triggers that are less commonly involved. Viral infection is the main asthma trigger in children. Active smoking is associated with higher asthma morbidity, and smoking cessation interventions should be personalized. Passive smoking is also a risk factor for asthma exacerbation. The implementation of public smoking bans has led to a reduction in the hospitalization of asthmatic children. Air pollution levels have been linked with asthmatic symptoms, a decrease in lung function, and increased emergency room visits and hospitalizations. Since avoidance is not easy to achieve, clean air policies remain the most effective strategy. Indoor air is also affected by air pollutants, such as cigarette smoke and

  11. Flight Deck Weather Avoidance Decision Support: Implementation and Evaluation

    NASA Technical Reports Server (NTRS)

    Wu, Shu-Chieh; Luna, Rocio; Johnson, Walter W.

    2013-01-01

    Weather related disruptions account for seventy percent of the delays in the National Airspace System (NAS). A key component in the weather plan of the Next Generation of Air Transportation System (NextGen) is to assimilate observed weather information and probabilistic forecasts into the decision process of flight crews and air traffic controllers. In this research we explore supporting flight crew weather decision making through the development of a flight deck predicted weather display system that utilizes weather predictions generated by ground-based radar. This system integrates and presents this weather information, together with in-flight trajectory modification tools, within a cockpit display of traffic information (CDTI) prototype. that the CDTI features 2D and perspective 3D visualization models of weather. The weather forecast products that we implemented were the Corridor Integrated Weather System (CIWS) and the Convective Weather Avoidance Model (CWAM), both developed by MIT Lincoln Lab. We evaluated the use of CIWS and CWAM for flight deck weather avoidance in two part-task experiments. Experiment 1 compared pilots' en route weather avoidance performance in four weather information conditions that differed in the type and amount of predicted forecast (CIWS current weather only, CIWS current and historical weather, CIWS current and forecast weather, CIWS current and forecast weather and CWAM predictions). Experiment 2 compared the use of perspective 3D and 21/2D presentations of weather for flight deck weather avoidance. Results showed that pilots could take advantage of longer range predicted weather forecasts in performing en route weather avoidance but more research will be needed to determine what combinations of information are optimal and how best to present them.

  12. Recurrent, Robust and Scalable Patterns Underlie Human Approach and Avoidance

    PubMed Central

    Kennedy, David N.; Lehár, Joseph; Lee, Myung Joo; Blood, Anne J.; Lee, Sang; Perlis, Roy H.; Smoller, Jordan W.; Morris, Robert; Fava, Maurizio

    2010-01-01

    Background Approach and avoidance behavior provide a means for assessing the rewarding or aversive value of stimuli, and can be quantified by a keypress procedure whereby subjects work to increase (approach), decrease (avoid), or do nothing about time of exposure to a rewarding/aversive stimulus. To investigate whether approach/avoidance behavior might be governed by quantitative principles that meet engineering criteria for lawfulness and that encode known features of reward/aversion function, we evaluated whether keypress responses toward pictures with potential motivational value produced any regular patterns, such as a trade-off between approach and avoidance, or recurrent lawful patterns as observed with prospect theory. Methodology/Principal Findings Three sets of experiments employed this task with beautiful face images, a standardized set of affective photographs, and pictures of food during controlled states of hunger and satiety. An iterative modeling approach to data identified multiple law-like patterns, based on variables grounded in the individual. These patterns were consistent across stimulus types, robust to noise, describable by a simple power law, and scalable between individuals and groups. Patterns included: (i) a preference trade-off counterbalancing approach and avoidance, (ii) a value function linking preference intensity to uncertainty about preference, and (iii) a saturation function linking preference intensity to its standard deviation, thereby setting limits to both. Conclusions/Significance These law-like patterns were compatible with critical features of prospect theory, the matching law, and alliesthesia. Furthermore, they appeared consistent with both mean-variance and expected utility approaches to the assessment of risk. Ordering of responses across categories of stimuli demonstrated three properties thought to be relevant for preference-based choice, suggesting these patterns might be grouped together as a relative preference

  13. A spatially explicit estimate of avoided forest loss.

    PubMed

    Honey-Rosés, Jordi; Baylis, Kathy; Ramírez, M Isabel

    2011-10-01

    With the potential expansion of forest conservation programs spurred by climate-change agreements, there is a need to measure the extent to which such programs achieve their intended results. Conventional methods for evaluating conservation impact tend to be biased because they do not compare like areas or account for spatial relations. We assessed the effect of a conservation initiative that combined designation of protected areas with payments for environmental services to conserve over wintering habitat for the monarch butterfly (Danaus plexippus) in Mexico. To do so, we used a spatial-matching estimator that matches covariates among polygons and their neighbors. We measured avoided forest loss (avoided disturbance and deforestation) by comparing forest cover on protected and unprotected lands that were similar in terms of accessibility, governance, and forest type. Whereas conventional estimates of avoided forest loss suggest that conservation initiatives did not protect forest cover, we found evidence that the conservation measures are preserving forest cover. We found that the conservation measures protected between 200 ha and 710 ha (3-16%) of forest that is high-quality habitat for monarch butterflies, but had a smaller effect on total forest cover, preserving between 0 ha and 200 ha (0-2.5%) of forest with canopy cover >70%. We suggest that future estimates of avoided forest loss be analyzed spatially to account for how forest loss occurs across the landscape. Given the forthcoming demand from donors and carbon financiers for estimates of avoided forest loss, we anticipate our methods and results will contribute to future studies that estimate the outcome of conservation efforts.

  14. Sexual selection as a consequence of pathogen avoidance behaviors

    SciTech Connect

    Loehle, C.; Logofet, D.O.

    1997-08-01

    The current theory that sexual selection results from female choice for good genes suffers from several problems. An alternative explanation is proposed. The pathogen avoidance hypothesis argues that the primary function of showy traits is to provide a reliable signal of current disease status so that sick individuals may be avoided during mating. Our studies shown that a significant risk of pathogen transmission occurs during mating and that showy traits are reliable indicators of current disease status. The origin of female choosiness is argued to lie in a general tendency to avoid sick individuals, even in the absence of showy traits. The showy traits are argued to originate as simple exaggerations of normal traits that are indicative of good health (bright feathers; vigorous movement; large size). Thus the origins of both showy traits and female choosiness are not problematic in this theory. A game theory analysis is employed to formalize the theory. Results of the game theory model support the theory. In particular, when the possession of male showy traits does not help reduce disease in the female, then showy traits are unlikely to occur. This case corresponds to the situation in large flocks or herds in which every animal is thoroughly exposed to all group pathogens on average. Such species do not exhibit showy traits. The good genes model does not make this prediction. The pathogen avoidance model can also lead to the evolution of showy traits even when selection is not effective against a given pathogen (e.g., when there is no heritable variation for resistance) but will lead to selection for resistance if such genes are present. Overall, the pathogen avoidance hypothesis provides a complete alternative to the good genes theory.

  15. Static electric field detection and behavioural avoidance in cockroaches.

    PubMed

    Newland, Philip L; Hunt, Edmund; Sharkh, Suleiman M; Hama, Noriyuki; Takahata, Masakazu; Jackson, Christopher W

    2008-12-01

    Electric fields are pervasively present in the environment and occur both as a result of man-made activities and through natural occurrence. We have analysed the behaviour of cockroaches to static electric fields and determined the physiological mechanisms that underlie their behavioural responses. The behaviour of animals in response to electric fields was tested using a Y-choice chamber with an electric field generated in one arm of the chamber. Locomotory behaviour and avoidance were affected by the magnitude of the electric fields with up to 85% of individuals avoiding the charged arm when the static electric field at the entrance to the arm was above 8-10 kV m(-1). Electric fields were found to cause a deflection of the antennae but when the antennae were surgically ablated, the ability of cockroaches to avoid electric fields was abolished. Fixation of various joints of the antennae indicated that hair plate sensory receptors at the base of the scape were primarily responsible for the detection of electric fields, and when antennal movements about the head-scape joint were prevented cockroaches failed to avoid electric fields. To overcome the technical problem of not being able to carry out electrophysiological analysis in the presence of electric fields, we developed a procedure using magnetic fields combined with the application of iron particles to the antennae to deflect the antennae and analyse the role of thoracic interneurones in signalling this deflection. The avoidance of electric fields in the context of high voltage power lines is discussed.

  16. Continuing food-avoidance diets after negative food challenges.

    PubMed

    Eigenmann, Philippe A; Caubet, Jean-Christoph; Zamora, Samuel A

    2006-12-01

    Negative food challenges for follow-up in patients previously diagnosed with food allergy should logically be followed by a normal diet. However, all patients do not reintroduce the food. The aims of the study were to define the proportion of negative food challenge not followed by a normal diet, and to identify possible reasons for not reintroducing the food. Patients with a negative food challenge were sent a questionnaire by mail. Items in the questionnaire included the symptoms at diagnosis, the duration of the diet, the fear of an accidental reaction during the avoidance diet and how it influenced the social life. Patients were also asked if the food was reintroduced after the negative food challenge, and if not, for which reasons. In 25.4% of the questionnaires (18/71) respondents reported that the food was not reintroduced. Patients with a previous diagnosis of peanut allergy tended to reintroduce the food less frequently than patients allergic to other foods. Girls were found to significantly less frequently reintroduce the food than boys. However, neither the severity of the initial reaction, the anxiety of an accidental reaction during the avoidance diet, nor a prolonged avoidance diet did influence the decision to reintroduce the food. Among other reasons listed, fears of persistence of allergies, with recurrent pruritus or non-specific skin rashes after eating the food, were reported in 12.7% of the total number of questionnaires. Patients who reintroduced the food reported that their social life generally improved. One quarter of previously allergic patients continue a food avoidance diet despite a negative challenge. We suggest reassessing food consumption in all patients after a negative food challenge, and in those still avoiding the specific food to consider a repeated challenge test.

  17. Programmable RNA recognition and cleavage by CRISPR/Cas9.

    PubMed

    O'Connell, Mitchell R; Oakes, Benjamin L; Sternberg, Samuel H; East-Seletsky, Alexandra; Kaplan, Matias; Doudna, Jennifer A

    2014-12-11

    The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA-DNA complementarity to identify target sites for sequence-specific double-stranded DNA (dsDNA) cleavage. In its native context, Cas9 acts on DNA substrates exclusively because both binding and catalysis require recognition of a short DNA sequence, known as the protospacer adjacent motif (PAM), next to and on the strand opposite the twenty-nucleotide target site in dsDNA. Cas9 has proven to be a versatile tool for genome engineering and gene regulation in a large range of prokaryotic and eukaryotic cell types, and in whole organisms, but it has been thought to be incapable of targeting RNA. Here we show that Cas9 binds with high affinity to single-stranded RNA (ssRNA) targets matching the Cas9-associated guide RNA sequence when the PAM is presented in trans as a separate DNA oligonucleotide. Furthermore, PAM-presenting oligonucleotides (PAMmers) stimulate site-specific endonucleolytic cleavage of ssRNA targets, similar to PAM-mediated stimulation of Cas9-catalysed DNA cleavage. Using specially designed PAMmers, Cas9 can be specifically directed to bind or cut RNA targets while avoiding corresponding DNA sequences, and we demonstrate that this strategy enables the isolation of a specific endogenous messenger RNA from cells. These results reveal a fundamental connection between PAM binding and substrate selection by Cas9, and highlight the utility of Cas9 for programmable transcript recognition without the need for tags.

  18. The dynamics of the RNA world: insights and challenges.

    PubMed

    Kun, Ádám; Szilágyi, András; Könnyű, Balázs; Boza, Gergely; Zachar, István; Szathmáry, Eörs

    2015-04-01

    The RNA world hypothesis of the origin of life, in which RNA emerged as both enzyme and information carrier, is receiving solid experimental support. The prebiotic synthesis of biomolecules, the catalytic aid offered by mineral surfaces, and the vast enzymatic repertoire of ribozymes are only pieces of the origin of life puzzle; the full picture can only emerge if the pieces fit together by either following from one another or coexisting with each other. Here, we review the theory of the origin, maintenance, and enhancement of the RNA world as an evolving population of dynamical systems. The dynamical view of the origin of life allows us to pinpoint the missing and the not fitting pieces: (1) How can the first self-replicating ribozyme emerge in the absence of template-directed information replication? (2) How can nucleotide replicators avoid competitive exclusion despite utilizing the very same resources (nucleobases)? (3) How can the information catastrophe be avoided? (4) How can enough genes integrate into a cohesive system in order to transition to a cellular stage? (5) How can the way information is stored and metabolic complexity coevolve to pave to road leading out of the RNA world to the present protein-DNA world?

  19. RNA silencing suppression by plant pathogens: defence, counter-defence and counter-counter-defence.

    PubMed

    Pumplin, Nathan; Voinnet, Olivier

    2013-11-01

    RNA silencing is a central regulator of gene expression in most eukaryotes and acts both at the transcriptional level through DNA methylation and at the post-transcriptional level through direct mRNA interference mediated by small RNAs. In plants and invertebrates, the same pathways also function directly in host defence against viruses by targeting viral RNA for degradation. Successful viruses have consequently evolved diverse mechanisms to avoid silencing, most notably through the expression of viral suppressors of RNA silencing. RNA silencing suppressors have also been recently identified in plant pathogenic bacteria and oomycetes, suggesting that disruption of host silencing is a general virulence strategy across several kingdoms of plant pathogens. There is also increasing evidence that plants have evolved specific defences against RNA-silencing suppression by pathogens, providing yet another illustration of the never-ending molecular arms race between plant pathogens and their hosts.

  20. A longitudinal analysis of self-regulation and well-being: avoidance personal goals, avoidance coping, stress generation, and subjective well-being.

    PubMed

    Elliot, Andrew J; Thrash, Todd M; Murayama, Kou

    2011-06-01

    We conducted 2 longitudinal meditational studies to test an integrative model of goals, stress and coping, and well-being. Study 1 documented avoidance personal goals as an antecedent of life stressors and life stressors as a partial mediator of the relation between avoidance goals and longitudinal change in subjective well-being (SWB). Study 2 fully replicated Study 1 and likewise validated avoidance goals as an antecedent of avoidance coping and avoidance coping as a partial mediator of the relation between avoidance goals and longitudinal change in SWB. It also showed that avoidance coping partially mediates the link between avoidance goals and life stressors and validated a sequential meditational model involving both avoidance coping and life stressors. The aforementioned results held when controlling for social desirability, basic traits, and general motivational dispositions. The findings are discussed with regard to the integration of various strands of research on self-regulation.