KSC-08pd3554

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Aircraft of the 920th Rescue Wing are lined up at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida to take part in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. They will join the U.S. Navy's Blue Angels for the show. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3553

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Aircraft of the 920th Rescue Wing are lined up at the Shuttle landing Facility at NASA's Kennedy Space Center in Florida to take part in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. They will join the U.S. Navy's Blue Angels for the show. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3602

NASA Image and Video Library

2008-11-08

CAPE CANAVERAL, Fla. – With the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida as a backdrop, this Castrol Aviator EXTRA 300SHP stunt plane, an unlimited-level aerobatic two-seat monoplane, performs aerobatic maneuvers during the Kennedy Space Center Visitor Complex Space and Air Show held Nov. 8-9. This year’s show brought together the best in military aircraft, such as the F/A-18 Super Hornet and F-16 Fighting Falcon, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event included a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Troy Cryder

[Development of medical emergency response system for accidents due to chemicals in Chongqing municipality].

PubMed

Ning, Xu; Dong, Zhao-jun; Mu, Ling; Zhai, Jian-cai

2006-12-01

To plan and develop a Chongqing chemical accident rescue command system. Based on the modes of leakage and diffusion of various poisonous gases and chemicals, different modes of injuries produced, and their appropriate rescue and treatments, also taking the following factors such as the condition of storage of chemicals, meteorological and geographic conditions, medical institutions and equipment, and their rescuing capacity into consideration, a plan was drafted to establish the rescue system. Real-time simulation technology, data analysis, evaluation technology and database technology were employed in the planning. Using Visual Studio 6.0 as the software development platform, this project aimed to design the software of an emergency command system for chemical accidents in Chongqing which could be operated with the Windows 2000/XP operating system. This system provided a dynamic scope of the endangered area, casualty number estimates, and recommendation of measures and a rescue plan for various chemical accidents. Furthermore, the system helped retrieve comprehensive information regarding the physical and chemical characteristics of more than 4 200 dangerous poisonous chemicals and their appropriate treatment modalities. This system is easy to operate with a friendly interface, functions rapidly and can provide real-time analysis with comparatively precise results. This system could satisfy the requirements of executing the command and the rescue of a chemical accident with good prospects of application.

Rescue of cardiac leptin receptors in db/db mice prevents myocardial triglyceride accumulation.

PubMed

Hall, Michael E; Maready, Matthew W; Hall, John E; Stec, David E

2014-08-01

Increased leptin levels have been suggested to contribute to cardiac hypertrophy and attenuate cardiac lipid accumulation in obesity, although it has been difficult to separate leptin's direct effects from those caused by changes in body weight and adiposity. To determine whether leptin attenuates cardiac lipid accumulation in obesity or directly causes left ventricular hypertrophy (LVH), we generated a novel mouse model in which the long form of the leptin receptor (LepR) was "rescued" only in cardiomyocytes of obese db/db mice. Reexpression of cardiomyocyte leptin receptors in db/db mice did not cause LVH but reduced cardiac triglycerides and improved cardiac function. Compared with lean wild-type (WT) or db/db-cardiac LepR rescue mice, db/db mice exhibited significantly lower E/A ratio, a measurement of early to late diastolic filling, which averaged 1.5 ± 0.07 in db/db vs. 1.9 ± 0.08 and 1.8 ± 0.11 in WT and db/db-cardiac LepR rescue mice, respectively. No differences in systolic function were observed. Although db/db and db/db-cardiac LepR rescue mice exhibited similar increases in plasma triglycerides, insulin, glucose, and body weight, cardiac triglycerides were significantly higher in db/db compared with WT and db/db cardiac LepR rescue mice, averaging 13.4 ± 4.2 vs. 3.8 ± 1.6 vs. 3.8 ± 0.7 mg/g, respectively. These results demonstrate that despite significant obesity and increases in plasma glucose and triglycerides, db/db cardiac LepR rescue mice are protected against myocardial lipid accumulation. However, we found no evidence that leptin directly causes LVH. Copyright © 2014 the American Physiological Society.

Psychiatric disorders after terrorist bombings among rescue workers and bombing survivors in Nairobi and rescue workers in Oklahoma City.

PubMed

Zhang, Gus; Pfefferbaum, Betty; Narayanan, Pushpa; Lee, Sungkyu; Thielman, Samuel; North, Carol S

2016-02-01

To examine the prevalence of psychopathology in 52 male rescue workers responding to the 1998 U.S. Embassy bombing in Nairobi, Kenya, comparing them with 176 male rescue workers responding to the 1995 Oklahoma City, Oklahoma, bombing and with 105 directly exposed male civilian survivors of the Nairobi bombing. The Diagnostic Interview Schedule/Disaster Supplement assessed pre-disaster and post-disaster psychiatric disorders and variables related to demographics, exposure, disaster perceptions, and coping in all 3 disaster subgroups. The most prevalent post-disaster disorders were posttraumatic stress disorder (PTSD) (22%) and major depressive disorder (MDD) (27%) among Nairobi rescue workers, which were more than 2 and 4 times higher, respectively, than among Oklahoma City rescue workers. Alcohol use disorder was the most prevalent pre- and post-disaster disorder among Oklahoma City rescue workers. Nairobi rescue workers had a prevalence of PTSD and MDD not significantly different from Nairobi civilian survivors. Nairobi rescue workers were more symptomatic than Oklahoma City rescue workers and were as symptomatic as Nairobi civilian survivors. The vulnerability of Nairobi rescue workers to psychological sequelae may be a reflection of their volunteer, rather than professional, status. These findings contribute to understanding rescue worker mental health, especially among volunteer rescue workers, with potential implications for the importance of professional status of rescue workers in conferring protection from adverse mental health outcomes.

Job task characteristics of Australian emergency services volunteers during search and rescue operations.

PubMed

Silk, Aaron; Lenton, Gavin; Savage, Robbie; Aisbett, Brad

2018-02-01

Search and rescue operations are necessary in locating, assisting and recovering individuals lost or in distress. In Australia, land-based search and rescue roles require a range of physically demanding tasks undertaken in dynamic and challenging environments. The aim of the current research was to identify and characterise the physically demanding tasks inherent to search and rescue operation personnel within Australia. These aims were met through a subjective job task analysis approach. In total, 11 criterion tasks were identified by personnel. These tasks were the most physically demanding, frequently occurring and operationally important tasks to these specialist roles. Muscular strength was the dominant fitness component for 7 of the 11 tasks. In addition to the discrete criterion tasks, an operational scenario was established. With the tasks and operational scenario identified, objective task analysis procedures can be undertaken so that practitioners can implement evidence-based strategies, such as physical selection procedures and task-based physical training programs, commensurate with the physical demands of search and rescue job roles. Practitioner Summary: The identification of physically demanding tasks amongst specialist emergency service roles predicates health and safety strategies which can be incorporated into organisations. Knowledge of physical task parameters allows employers to mitigate injury risk through the implementation of strategies modelled on the precise physical demands of the role.

Precise Correction of Disease Mutations in Induced Pluripotent Stem Cells Derived From Patients With Limb Girdle Muscular Dystrophy.

PubMed

Turan, Soeren; Farruggio, Alfonso P; Srifa, Waracharee; Day, John W; Calos, Michele P

2016-04-01

Limb girdle muscular dystrophies types 2B (LGMD2B) and 2D (LGMD2D) are degenerative muscle diseases caused by mutations in the dysferlin and alpha-sarcoglycan genes, respectively. Using patient-derived induced pluripotent stem cells (iPSC), we corrected the dysferlin nonsense mutation c.5713C>T; p.R1905X and the most common alpha-sarcoglycan mutation, missense c.229C>T; p.R77C, by single-stranded oligonucleotide-mediated gene editing, using the CRISPR/Cas9 gene-editing system to enhance the frequency of homology-directed repair. We demonstrated seamless, allele-specific correction at efficiencies of 0.7-1.5%. As an alternative, we also carried out precise gene addition strategies for correction of the LGMD2B iPSC by integration of wild-type dysferlin cDNA into the H11 safe harbor locus on chromosome 22, using dual integrase cassette exchange (DICE) or TALEN-assisted homologous recombination for insertion precise (THRIP). These methods employed TALENs and homologous recombination, and DICE also utilized site-specific recombinases. With DICE and THRIP, we obtained targeting efficiencies after selection of ~20%. We purified iPSC corrected by all methods and verified rescue of appropriate levels of dysferlin and alpha-sarcoglycan protein expression and correct localization, as shown by immunoblot and immunocytochemistry. In summary, we demonstrate for the first time precise correction of LGMD iPSC and validation of expression, opening the possibility of cell therapy utilizing these corrected iPSC.

Recent progress in translational cystic fibrosis research using precision medicine strategies.

PubMed

Cholon, Deborah M; Gentzsch, Martina

2018-03-01

Significant progress has been achieved in developing precision therapies for cystic fibrosis; however, highly effective treatments that target the ion channel, CFTR, are not yet available for many patients. As numerous CFTR therapeutics are currently in the clinical pipeline, reliable screening tools capable of predicting drug efficacy to support individualized treatment plans and translational research are essential. The utilization of bronchial, nasal, and rectal tissues from individual cystic fibrosis patients for drug testing using in vitro assays such as electrophysiological measurements of CFTR activity and evaluation of fluid movement in spheroid cultures, has advanced the prediction of patient-specific responses. However, for precise prediction of drug effects, in vitro models of CFTR rescue should incorporate the inflamed cystic fibrosis airway environment and mimic the complex tissue structures of airway epithelia. Furthermore, novel assays that monitor other aspects of successful CFTR rescue such as restoration of mucus characteristics, which is important for predicting mucociliary clearance, will allow for better prognoses of successful therapies in vivo. Additional cystic fibrosis treatment strategies are being intensively explored, such as development of drugs that target other ion channels, and novel technologies including pluripotent stem cells, gene therapy, and gene editing. The multiple therapeutic approaches available to treat the basic defect in cystic fibrosis combined with relevant precision medicine models provide a framework for identifying optimal and sustained treatments that will benefit all cystic fibrosis patients. Copyright © 2017 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

Locating Direction Finders in a Generalized Search and Rescue Network

DTIC Science & Technology

1991-03-01

David A . Drake and Alfred B. Marsb. Conv-ersation at NationalI Security Agenc, 29 September 1990. 7. Daskin , Mark S. " A M-admum Fpeced Co-:efing...91 7 19 134 .flT/GORjEnSj9I-M LOCATING DIRECTION FIND’RS IN A GENERALIZED SEARCH AND RESCUE NETWORK THESIS Jean M. Steppe Captain, USAF AFIT/GOR/EN S...91-Mk-7 APPROVED FOR PUBLIC RELI ASE: DISTRIBUTION UNLIMITED. 1191-05734 .. 91.. .7 19 134 i i . nMIGOR4M.S/91-MI LOCATING DIRECTION FINDERS IW A

KSC-08pd3577

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's F/A-18 Blue Angels take off from the runway at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida to begin rehearsing their demonstrations for the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event also includes demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

A Framework for Intelligent Battlefield Treatment System

NASA Astrophysics Data System (ADS)

Yu, Jian; Wu, Biao; Yi, Renjie; Zhu, Jie

With the development of high technology weapon, the execution and precision of weapon have made great improvement, which arouse many new challenge for treatment of war wounds. It is very urgent to research how to reduce casualty of individual solider. However, researches focus only on daily application recently. This paper discusses Intelligent Battlefield Treatment System (IBTS), and designs the framework of the service-oriented system based on WCF. The system has the following functions: testing the physical condition of a solider, disposing the distress signals intelligently, dispatching rescuers and assisting self-rescue or mutual-rescue to the wounded. The IBTS characteristics of data aggregate, multi-platform operation and data sharing can improve the treatment efficiency.

Developing a new perspective to study the health of survivors of Sichuan earthquakes in China: a study on the effect of post-earthquake rescue policies on survivors’ health-related quality of life

PubMed Central

2013-01-01

Background Sichuan is a province in China with an extensive history of earthquakes. Recent earthquakes, including the Lushan earthquake in 2013, have resulted in thousands of people losing their homes and their families. However, there is a research gap on the efficiency of government support policies. Therefore, this study develops a new perspective to study the health of earthquake survivors, based on the effect of post-earthquake rescue policies on health-related quality of life (HRQOL) of survivors of the Sichuan earthquake. Methods This study uses data from a survey conducted in five hard-hit counties (Wenchuan, Qingchuan, Mianzhu, Lushan, and Dujiangyan) in Sichuan in 2013. A total of 2,000 questionnaires were distributed, and 1,672 were returned; the response rate was 83.6%. Results Results of the rescue policies scale and Medical Outcomes Study Short Form 36 (SF-36) scale passed the reliability test. The confirmatory factor analysis model showed that the physical component summary (PCS) directly affected the mental component summary (MCS). The results of structural equation model regarding the effects of rescue policies on HRQOL showed that the path coefficients of six policies (education, orphans, employment, poverty, legal, and social rescue policies) to the PCS of survivors were all positive and passed the test of significance. Finally, although only the path coefficient of the educational rescue policy to the MCS of survivors was positive and passed the test of significance, the other five policies affected the MCS indirectly through the PCS. Conclusions The general HRQOL of survivors is not ideal; the survivors showed a low satisfaction with the post-earthquake rescue policies. Further, the six post-earthquake rescue policies significantly improved the HRQOL of survivors and directly affected the promotion of the PCS of survivors. Aside from the educational rescue policy, all other policies affected the MCS indirectly through the PCS. This finding indicates relatively large differences in the effects of different post-earthquake rescue policies on the HRQOL of survivors. PMID:24168028

Developing a new perspective to study the health of survivors of Sichuan earthquakes in China: a study on the effect of post-earthquake rescue policies on survivors' health-related quality of life.

PubMed

Liang, Ying; Wang, Xiukun

2013-10-29

Sichuan is a province in China with an extensive history of earthquakes. Recent earthquakes, including the Lushan earthquake in 2013, have resulted in thousands of people losing their homes and their families. However, there is a research gap on the efficiency of government support policies. Therefore, this study develops a new perspective to study the health of earthquake survivors, based on the effect of post-earthquake rescue policies on health-related quality of life (HRQOL) of survivors of the Sichuan earthquake. This study uses data from a survey conducted in five hard-hit counties (Wenchuan, Qingchuan, Mianzhu, Lushan, and Dujiangyan) in Sichuan in 2013. A total of 2,000 questionnaires were distributed, and 1,672 were returned; the response rate was 83.6%. Results of the rescue policies scale and Medical Outcomes Study Short Form 36 (SF-36) scale passed the reliability test. The confirmatory factor analysis model showed that the physical component summary (PCS) directly affected the mental component summary (MCS). The results of structural equation model regarding the effects of rescue policies on HRQOL showed that the path coefficients of six policies (education, orphans, employment, poverty, legal, and social rescue policies) to the PCS of survivors were all positive and passed the test of significance. Finally, although only the path coefficient of the educational rescue policy to the MCS of survivors was positive and passed the test of significance, the other five policies affected the MCS indirectly through the PCS. The general HRQOL of survivors is not ideal; the survivors showed a low satisfaction with the post-earthquake rescue policies. Further, the six post-earthquake rescue policies significantly improved the HRQOL of survivors and directly affected the promotion of the PCS of survivors. Aside from the educational rescue policy, all other policies affected the MCS indirectly through the PCS. This finding indicates relatively large differences in the effects of different post-earthquake rescue policies on the HRQOL of survivors.

Pro-social behaviour of ants depends on their ecological niche-Rescue actions in species from tropical and temperate regions.

PubMed

Miler, Krzysztof; Yahya, Bakhtiar Effendi; Czarnoleski, Marcin

2017-11-01

Some ants display rescue behaviour, which is performed by nearby nestmates and directed at individuals in danger. Here, using several ant species, we demonstrate that rescue behaviour expression matches predicted occurrences based on certain aspects of species' ecological niches. Rescue occurred in sand-dwelling ants exposed both to co-occurring antlion larvae, representing the threat of being captured by a predator, and to nest cave-ins, representing the threat of being trapped in a collapsed nest chamber. Rescue also occurred in forest groundcover ants exposed to certain entrapment situations. However, rescue never occurred in species associated with open plains, which nest in hardened soils and forage largely on herbaceous plants, or in ants living in close mutualistic relationships with their host plants. In addition, because we tested each species in two types of tests, antlion larva capture tests and artificial entrapment tests, we highlight the importance of accounting for test context in studying rescue behaviour expression. Copyright © 2017 Elsevier B.V. All rights reserved.

Communication-dependent mineralization of osteoblasts via gap junctions.

PubMed

Hashida, Yukihiko; Nakahama, Ken-ichi; Shimizu, Kaori; Akiyama, Masako; Harada, Kiyoshi; Morita, Ikuo

2014-04-01

Connexin43 (Cx43) is a major gap junction (GJ) protein in bone and plays a critical role in osteoblast differentiation. Several studies show that osteoblast differentiation is delayed by Cx43 ablation. However, the precise mechanism underlying the role of Cx43 in osteoblast differentiation is not fully understood. Firstly, we analyzed the phenotype of a conditional knockout mouse, which was generated by mating of an osterix promoter-driven Cre expressing mouse with a Cx43-floxed mouse. As expected, delayed ossification was observed. Secondly, we demonstrated that the cell communication via gap junctions played an important role in osteoblast differentiation using a tamoxifen-inducible knockout system in vitro. Genetic ablation of Cx43 resulted in both the disruption of cell-communications and the attenuation of osteoblast mineralization induced by BMP-2, but not by ascorbic acid. Moreover, restoring full-length Cx43 (382aa) expression rescued the impairment of osteoblast cell-communication and osteoblast mineralization; however, the expression of the Cx43 N-terminal mutant (382aaG2V) did not rescue either of them. Comparing the gene expression profiles, the genes directly regulated by BMP-2 were attenuated by Cx43 gene ablation. These results suggested that the cell-communication mediated by gap junctions was indispensable for normal differentiation of osteoblast induced by BMP-2. Copyright © 2013 Elsevier Inc. All rights reserved.

KSC-08pd3573

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Military aircraft are lined up on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. The F/A-18s, F-4, F-16 and F-104G Starfighter will take part in demonstrations at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron, the Blue Angels, will take to the skies in military aircraft demonstrations for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event also includes demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3575

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's F/A-18 Blue Angels begin taxiing toward the runway at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. They will taking off to begin rehearsing their demonstrations for the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event also includes demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3548

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's Blue Angels approach the Shuttle Landing Facility for a landing at NASA's Kennedy Space Center in Florida. They will perform at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3576

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's F/A-18 Blue Angels taxi toward the runway at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. They will taking off to begin rehearsing their demonstrations for the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event also includes demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3550

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's Blue Angels demonstration squadron lands at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. They will perform at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3572

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's F/A-18 Blue Angels are lined up on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. The Blue Angels and other aircraft are at Kennedy for demonstrations at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event also includes demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3574

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's F/A-18 Blue Angels (left) and other military aircraft fire their engines on the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida before taking off. They will be rehearsing their demonstrations for the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event also includes demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

[Emergency response management near the tracks of the public railway network: special aspects of missions connected with the German national railway system].

PubMed

Krämer, P; Aul, A; Vock, B; Frank, C

2010-11-01

Emergency response management and rescue operations concerning the railway network in Germany need special attention and implementation in several ways. The emergency response concerning the German national railway network managed by Deutsche Bahn AG is subject to various rules and regulations which have to be followed precisely. Only by following these rules and procedures is the safety of all emergency staff at the scene ensured. The German national railway network (Deutsche Bahn AG) provides its own emergency response control center, which specializes in managing its response to emergencies and dispatches an emergency response manager to the scene. This person serves as the primary Deutsche Bahn AG representative at the scene and is the only person who is allowed to earth the railway electrical power lines. This article will discuss different emergency situations concerning railway accidents and the emergency medical response to them based on a near collision with a high speed train during a rescue mission close to the railway track. Injury to personnel could only be avoided by chance and luck. The dangers and risks for rescue staff are specified. Furthermore, the article details practical guidelines for rescue operations around the German national railway track system.

Factors associated with post-traumatic stress symptoms among adolescents exposed to the Sewol ferry disaster in Korea.

PubMed

Lee, Ju-Yeon; Kim, Sung-Wan; Bae, Kyung-Yeol; Kim, Jae-Min; Shin, Il-Seon; Yoon, Jin-Sang

2017-10-01

This study evaluated the factors associated with post-traumatic stress symptoms in Korean adolescents who lived in a disaster-affected community. A total of 1101 students attending secondary and high schools in Jindo, the location of the Sewol ferry disaster, were enrolled in a cross-sectional survey. The Child Report of Post-traumatic Symptoms (CROPS), the Center for Epidemiological Studies Depression Scale (CES-D), and the State Anxiety Inventory for Children (SAIC) were administered. Female gender, older children, poor academic achievement, and directly witnessing the rescue scene were associated with post-traumatic stress symptoms. The CES-D and SAIC scores of subjects with witness of the rescue were significantly higher than those of respondents without such experiences. The regression analysis revealed that directly witnessing the rescue scene was significantly associated with post-traumatic stress symptoms after adjusting for other variables. The results of this study suggest that witnessing the rescue scene following a disaster might be a risk factor for post-traumatic stress symptoms in adolescents in disaster-affected communities. Copyright © 2017 Elsevier B.V. All rights reserved.

Direct Actions of Kisspeptins on GnRH Neurons Permit Attainment of Fertility but are Insufficient to Fully Preserve Gonadotropic Axis Activity

PubMed Central

León, Silvia; Barroso, Alexia; Vázquez, María J.; García-Galiano, David; Manfredi-Lozano, María; Ruiz-Pino, Francisco; Heras, Violeta; Romero-Ruiz, Antonio; Roa, Juan; Schutz, Günther; Kirilov, Milen; Gaytan, Francisco; Pinilla, Leonor; Tena-Sempere, Manuel

2016-01-01

Kisspeptins, ligands of the receptor, Gpr54, are potent stimulators of puberty and fertility. Yet, whether direct kisspeptin actions on GnRH neurons are sufficient for the whole repertoire of their reproductive effects remains debatable. To dissect out direct vs. indirect effects of kisspeptins on GnRH neurons in vivo, we report herein the detailed reproductive/gonadotropic characterization of a Gpr54 null mouse line with selective re-introduction of Gpr54 expression only in GnRH cells (Gpr54−/−Tg; rescued). Despite preserved fertility, adult rescued mice displayed abnormalities in gonadal microstructure, with signs of precocious ageing in females and elevated LH levels with normal-to-low testosterone secretion in males. Gpr54−/−Tg rescued mice showed also altered gonadotropin responses to negative feedback withdrawal, while luteinizing hormone responses to various gonadotropic regulators were variably affected, with partially blunted relative (but not absolute) responses to kisspeptin-10, NMDA and the agonist of tachykinin receptors, NK2R. Our data confirm that direct effects of kisspeptins on GnRH cells are sufficient to attain fertility. Yet, such direct actions appear to be insufficient to completely preserve proper functionality of gonadotropic axis, suggesting a role of kisspeptin signaling outside GnRH cells. PMID:26755241

KSC-08pd3556

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's Blue Angels F/A-18 jets fly in formation over the runway at NASA's Kennedy Space Center in Florida before landing. They are at Kennedy to perform in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3558

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – The U.S. Navy's Blue Angels F/A-18 jet No. 1 lands on the runway at NASA's Kennedy Space Center in Florida. The Blue Angels are at Kennedy to perform in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3561

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – An F-4 jet aircraft lands at NASA's Kennedy Space Center in Florida. It will join the U.S. Navy's Blue Angels and other aircraft for demonstrations at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3559

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Two F/A-18 jets fly over the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. They will join the U.S. Navy's Blue Angels and other aircraft for demonstrations at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3562

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Three of the U.S. Navy's Blue Angels F/A-18 jets fly over the runway at NASA's Kennedy Space Center in Florida. The Blue Angels are at Kennedy to perform in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3560

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – An F/A-18 jet lands at NASA's Kennedy Space Center in Florida. It will join the U.S. Navy's Blue Angels and other aircraft for demonstrations at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

Magnet and Semi Precision Attachment in an Implant Retained Partial Denture for the Rehabilitation of an Irradiated Marginal Mandibulectomy Patient: A Case Report

PubMed Central

Guttal, Satyabodh Shesharaj; Kulkarni, Sudhindra S; Kudva, Adarsh; Thakur, Srinath

2015-01-01

Surgical treatment of malignancies in the oral cavity (mandible, tongue, floor of the mouth, alveolus, buccal sulcus) often results in an unfavourable anatomic condition for prosthodontic rehabilitation. Hence, maxillofacial prosthetic rehabilitation becomes a mightier task when resection is accompanied by radiation therapy. In selected cases, implant therapy comes to rescue. The following report throws light on the case of prosthetic rehabilitation of a patient who underwent right marginal mandibulectomy and right partial glossectomy, with the aid of a single implant, semi precision attachment and magnet supported partial denture. PMID:26501028

Psychiatric disorders in rescue workers after the Oklahoma City bombing.

PubMed

North, Carol S; Tivis, Laura; McMillen, J Curtis; Pfefferbaum, Betty; Spitznagel, Edward L; Cox, Jann; Nixon, Sara; Bunch, Kenneth P; Smith, Elizabeth M

2002-05-01

Psychiatric disorders were studied in a volunteer group of 181 firefighters who served as rescue/recovery workers after the Oklahoma City bombing. Approximately 34 months after the disaster, the authors retrospectively assessed psychopathology both before and after the bombing with the Diagnostic Interview Schedule. Findings for male rescue workers were compared with those of male primary victims who had been in the direct path of the blast and who had been assessed with the same instrument. The prevalence of posttraumatic stress disorder related to the bombing was significantly lower in male rescue workers (13%) than in male primary victims (23%). High rates of alcohol disorders (postdisaster: 24%; lifetime: 47%) were seen among all rescue workers, with virtually no new cases occurring after the bombing. The resilience seen in firefighters may be related to their career selection, their preparedness and experience, the fewer injuries they suffered, and postdisaster mental health interventions. However, alcohol disorders were endemic before the disaster, indicating a need for ongoing programs targeting this problem.

Lightweight Boom For Rescue Helicopter

NASA Technical Reports Server (NTRS)

Haslim, Leonard A.

1993-01-01

Telescoping boom and associated mechanisms attached to helicopter aid rescue operations by extending lifeline beyond sweep of main rotor. Pilot observes rescuee and control position of helicopter more effectively than if rescuee directly below and hidden from pilot's view. Rescuee outside downdraft of rotor, which is often powerful enough to blow away or submerge someone in water. Used for marine or land operations. Boom thin and lightweight because it need not support weight of rescuee. Lifeline pulls away from boom after secured around rescuee, who is lifted directly into cabin by winch. Potential application for in situ erection of telescopic space structures.

Conservation and the 4 Rs, which are rescue, rehabilitation, release, and research.

PubMed

Pyke, Graham H; Szabo, Judit K

2018-02-01

Vertebrate animals can be injured or threatened with injury through human activities, thus warranting their "rescue." Details of wildlife rescue, rehabilitation, release, and associated research (our 4 Rs) are often recorded in large databases, resulting in a wealth of available information. This information has huge research potential and can contribute to understanding of animal biology, anthropogenic impacts on wildlife, and species conservation. However, such databases have been little used, few studies have evaluated factors influencing success of rehabilitation and/or release, recommended actions to conserve threatened species have rarely arisen, and direct benefits for species conservation are yet to be demonstrated. We therefore recommend that additional research be based on data from rescue, rehabilitation, and release of animals that is broader in scope than previous research and would have community support. © 2017 Society for Conservation Biology.

Mib1 contributes to persistent directional cell migration by regulating the Ctnnd1-Rac1 pathway.

PubMed

Mizoguchi, Takamasa; Ikeda, Shoko; Watanabe, Saori; Sugawara, Michiko; Itoh, Motoyuki

2017-10-31

Persistent directional cell migration is involved in animal development and diseases. The small GTPase Rac1 is involved in F-actin and focal adhesion dynamics. Local Rac1 activity is required for persistent directional migration, whereas global, hyperactivated Rac1 enhances random cell migration. Therefore, precise control of Rac1 activity is important for proper directional cell migration. However, the molecular mechanism underlying the regulation of Rac1 activity in persistent directional cell migration is not fully understood. Here, we show that the ubiquitin ligase mind bomb 1 (Mib1) is involved in persistent directional cell migration. We found that knockdown of MIB1 led to an increase in random cell migration in HeLa cells in a wound-closure assay. Furthermore, we explored novel Mib1 substrates for cell migration and found that Mib1 ubiquitinates Ctnnd1. Mib1-mediated ubiquitination of Ctnnd1 K547 attenuated Rac1 activation in cultured cells. In addition, we found that posterior lateral line primordium cells in the zebrafish mib1 ta52b mutant showed increased random migration and loss of directional F-actin-based protrusion formation. Knockdown of Ctnnd1 partially rescued posterior lateral line primordium cell migration defects in the mib1 ta52b mutant. Taken together, our data suggest that Mib1 plays an important role in cell migration and that persistent directional cell migration is regulated, at least in part, by the Mib1-Ctnnd1-Rac1 pathway. Published under the PNAS license.

Simulation and analysis of differential GPS

NASA Astrophysics Data System (ADS)

Denaro, R. P.

NASA is conducting a research program to evaluate differential Global Positioning System (GPS) concepts for civil helicopter navigation. It is pointed out that the civil helicopter community will probably be an early user of GPS because of the unique mission operations in areas where precise navigation aids are not available. However, many of these applications involve accuracy requirements which cannot be satisfied by conventional GPS. Such applications include remote area search and rescue, offshore oil platform approach, remote area precision landing, and other precise navigation operations. Differential GPS provides a promising approach for meeting very demanding accuracy requirements. The considered procedure eliminates some of the common bias errors experienced by conventional GPS. This is done by making use of a second GPS receiver. A simulation process is developed as a tool for analyzing various scenarios of GPS-referenced civil aircraft navigation.

KSC-08pd3563

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Three of the U.S. Navy's Blue Angels F/A-18 jets fly in formation over the runway before landing at NASA's Kennedy Space Center in Florida. The Blue Angels are at Kennedy to perform in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3547

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – A C-130 airplane flown by U.S. Marines lands at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. The plane carries the support team for the U.S. Navy's Blue Angels, who are going to perform at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3557

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – Storks seem to join the formation as the U.S. Navy's Blue Angels F/A-18 jets land on the runway at NASA's Kennedy Space Center in Florida. The Blue Angels are at Kennedy to perform in the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3549

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – A C-130 airplane flown by U.S. Marines stops at the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida. The plane carries the support team for the U.S. Navy's Blue Angels, who are going to perform at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3551

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – At the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, ground support personnel work on an F/A-18, one of the U.S. Navy's Blue Angels aircraft. The demonstration squadron will perform at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

KSC-08pd3606

NASA Image and Video Library

2008-11-08

CAPE CANAVERAL, Fla. – During a flight aboard the U.S. Navy Blue Angel's support aircraft, a C-130T Hercules known as "Fat Albert," the photographer was able to capture the entire Kennedy Space Center Visitor Complex. Well-known structures are the genuine, full-size solid rocket boosters and external tank at left, Astronaut Memorial Mirror at center in the foreground, and the Rocket Garden at center right. The Blue Angels performed during the Kennedy Space Center Visitor Complex Space and Air Show held Nov. 8-9. This year’s show brought together the best in military aircraft, such as the F/A-18 Super Hornet and F-16 Fighting Falcon, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event included a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Ben Smegelsky

Gazing toward humans: a study on water rescue dogs using the impossible task paradigm.

PubMed

D'Aniello, Biagio; Scandurra, Anna; Prato-Previde, Emanuela; Valsecchi, Paola

2015-01-01

Various studies have assessed the role of life experiences, including learning opportunities, living conditions and the quality of dog-human relationships, in the use of human cues and problem-solving ability. The current study investigates how and to what extent training affects the behaviour of dogs and the communication of dogs with humans by comparing dogs trained for a water rescue service and untrained pet dogs in the impossible task paradigm. Twenty-three certified water rescue dogs (the water rescue group) and 17 dogs with no training experience (the untrained group) were tested using a modified version of the impossible task described by Marshall-Pescini et al. in 2009. The results demonstrated that the water rescue dogs directed their first gaze significantly more often towards the owner and spent more time gazing toward two people compared to the untrained pet dogs. There was no difference between the dogs of the two groups as far as in the amount of time spent gazing at the owner or the stranger; neither in the interaction with the apparatus attempting to obtain food. The specific training regime, aimed at promoting cooperation during the performance of water rescue, could account for the longer gazing behaviour shown toward people by the water rescue dogs and the priority of gazing toward the owner. Copyright © 2014 Elsevier B.V. All rights reserved.

Soluble factor(s) from bone marrow cells can rescue lethally irradiated mice by protecting endogenous hematopoietic stem cells.

PubMed

Zhao, Yi; Zhan, Yuxia; Burke, Kathleen A; Anderson, W French

2005-04-01

Ionizing radiation-induced myeloablation can be rescued via bone marrow transplantation (BMT) or administration of cytokines if given within 2 hours after radiation exposure. There is no evidence for the existence of soluble factors that can rescue an animal after a lethal dose of radiation when administered several hours postradiation. We established a system that could test the possibility for the existence of soluble factors that could be used more than 2 hours postirradiation to rescue animals. Animals with an implanted TheraCyte immunoisolation device (TID) received lethal-dose radiation and then normal bone marrow Lin- cells were loaded into the device (thereby preventing direct interaction between donor and recipient cells). Animal survival was evaluated and stem cell activity was tested with secondary bone marrow transplantation and flow cytometry analysis. Donor cell gene expression of five antiapoptotic cytokines was examined. Bone marrow Lin- cells rescued lethally irradiated animals via soluble factor(s). Bone marrow cells from the rescued animals can rescue and repopulate secondary lethally irradiated animals. Within the first 6 hours post-lethal-dose radiation, there is no significant change of gene expression of the known radioprotective factors TPO, SCF, IL-3, Flt-3 ligand, and SDF-1. Hematopoietic stem cells can be protected in lethally irradiated animals by soluble factors produced by bone marrow Lin- cells.

Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.

PubMed

Michalkiewicz, Mieczyslaw; Michalkiewicz, Teresa; Ettinger, Ruth A; Rutledge, Elizabeth A; Fuller, Jessica M; Moralejo, Daniel H; Van Yserloo, Brian; MacMurray, Armand J; Kwitek, Anne E; Jacob, Howard J; Lander, Eric S; Lernmark, Ake

2004-10-04

A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.

A learning-based semi-autonomous controller for robotic exploration of unknown disaster scenes while searching for victims.

PubMed

Doroodgar, Barzin; Liu, Yugang; Nejat, Goldie

2014-12-01

Semi-autonomous control schemes can address the limitations of both teleoperation and fully autonomous robotic control of rescue robots in disaster environments by allowing a human operator to cooperate and share such tasks with a rescue robot as navigation, exploration, and victim identification. In this paper, we present a unique hierarchical reinforcement learning-based semi-autonomous control architecture for rescue robots operating in cluttered and unknown urban search and rescue (USAR) environments. The aim of the controller is to enable a rescue robot to continuously learn from its own experiences in an environment in order to improve its overall performance in exploration of unknown disaster scenes. A direction-based exploration technique is integrated in the controller to expand the search area of the robot via the classification of regions and the rubble piles within these regions. Both simulations and physical experiments in USAR-like environments verify the robustness of the proposed HRL-based semi-autonomous controller to unknown cluttered scenes with different sizes and varying types of configurations.

Evolution of synthetic signaling scaffolds by recombination of modular protein domains.

PubMed

Lai, Andicus; Sato, Paloma M; Peisajovich, Sergio G

2015-06-19

Signaling scaffolds are proteins that interact via modular domains with multiple partners, regulating signaling networks in space and time and providing an ideal platform from which to alter signaling functions. However, to better exploit scaffolds for signaling engineering, it is necessary to understand the full extent of their modularity. We used a directed evolution approach to identify, from a large library of randomly shuffled protein interaction domains, variants capable of rescuing the signaling defect of a yeast strain in which Ste5, the scaffold in the mating pathway, had been deleted. After a single round of selection, we identified multiple synthetic scaffold variants with diverse domain architectures, able to mediate mating pathway activation in a pheromone-dependent manner. The facility with which this signaling network accommodates changes in scaffold architecture suggests that the mating signaling complex does not possess a single, precisely defined geometry into which the scaffold has to fit. These relaxed geometric constraints may facilitate the evolution of signaling networks, as well as their engineering for applications in synthetic biology.

Arenavirus reverse genetics for vaccine development

PubMed Central

Ortiz-Riaño, Emilio; Cheng, Benson Yee Hin; Carlos de la Torre, Juan

2013-01-01

Arenaviruses are important human pathogens with no Food and Drug Administration (FDA)-licensed vaccines available and current antiviral therapy being limited to an off-label use of the nucleoside analogue ribavirin of limited prophylactic efficacy. The development of reverse genetics systems represented a major breakthrough in arenavirus research. However, rescue of recombinant arenaviruses using current reverse genetics systems has been restricted to rodent cells. In this study, we describe the rescue of recombinant arenaviruses from human 293T cells and Vero cells, an FDA-approved line for vaccine development. We also describe the generation of novel vectors that mediate synthesis of both negative-sense genome RNA and positive-sense mRNA species of lymphocytic choriomeningitis virus (LCMV) directed by the human RNA polymerases I and II, respectively, within the same plasmid. This approach reduces by half the number of vectors required for arenavirus rescue, which could facilitate virus rescue in cell lines approved for human vaccine production but that cannot be transfected at high efficiencies. We have shown the feasibility of this approach by rescuing both the Old World prototypic arenavirus LCMV and the live-attenuated vaccine Candid#1 strain of the New World arenavirus Junín. Moreover, we show the feasibility of using these novel strategies for efficient rescue of recombinant tri-segmented both LCMV and Candid#1. PMID:23364194

A Review of Mine Rescue Ensembles for Underground Coal Mining in the United States.

PubMed

Kilinc, F Selcen; Monaghan, William D; Powell, Jeffrey B

The mining industry is among the top ten industries nationwide with high occupational injury and fatality rates, and mine rescue response may be considered one of the most hazardous activities in mining operations. In the aftermath of an underground mine fire, explosion or water inundation, specially equipped and trained teams have been sent underground to fight fires, rescue entrapped miners, test atmospheric conditions, investigate the causes of the disaster, or recover the dead. Special personal protective ensembles are used by the team members to improve the protection of rescuers against the hazards of mine rescue and recovery. Personal protective ensembles used by mine rescue teams consist of helmet, cap lamp, hood, gloves, protective clothing, boots, kneepads, facemask, breathing apparatus, belt, and suspenders. While improved technology such as wireless warning and communication systems, lifeline pulleys, and lighted vests have been developed for mine rescuers over the last 100 years, recent research in this area of personal protective ensembles has been minimal due to the trending of reduced exposure of rescue workers. In recent years, the exposure of mine rescue teams to hazardous situations has been changing. However, it is vital that members of the teams have the capability and proper protection to immediately respond to a wide range of hazardous situations. Currently, there are no minimum requirements, best practice documents, or nationally recognized consensus standards for protective clothing used by mine rescue teams in the United States (U.S.). The following review provides a summary of potential issues that can be addressed by rescue teams and industry to improve potential exposures to rescue team members should a disaster situation occur. However, the continued trending in the mining industry toward non-exposure to potential hazards for rescue workers should continue to be the primary goal. To assist in continuing this trend, the mining industry and regulatory agencies have been more restrictive by requiring additional post disaster information regarding atmospheric conditions and other hazards before exposing rescue workers and others in the aftermath of a mine disaster. In light of some of the more recent mine rescuer fatalities such as the Crandall Canyon Mine and Jim Walters Resources in the past years, the direction of reducing exposure is preferred. This review provides a historical perspective on ensembles used during mine rescue operations and summarizes environmental hazards, critical elements of mine rescue ensembles, and key problems with these elements. This study also identifies domains for improved mine rescue ensembles. Furthermore, field observations from several coal mine rescue teams were added to provide the information on the currently used mine rescue ensembles in the U.S.

A Review of Mine Rescue Ensembles for Underground Coal Mining in the United States

PubMed Central

Kilinc, F. Selcen; Monaghan, William D.; Powell, Jeffrey B.

2016-01-01

The mining industry is among the top ten industries nationwide with high occupational injury and fatality rates, and mine rescue response may be considered one of the most hazardous activities in mining operations. In the aftermath of an underground mine fire, explosion or water inundation, specially equipped and trained teams have been sent underground to fight fires, rescue entrapped miners, test atmospheric conditions, investigate the causes of the disaster, or recover the dead. Special personal protective ensembles are used by the team members to improve the protection of rescuers against the hazards of mine rescue and recovery. Personal protective ensembles used by mine rescue teams consist of helmet, cap lamp, hood, gloves, protective clothing, boots, kneepads, facemask, breathing apparatus, belt, and suspenders. While improved technology such as wireless warning and communication systems, lifeline pulleys, and lighted vests have been developed for mine rescuers over the last 100 years, recent research in this area of personal protective ensembles has been minimal due to the trending of reduced exposure of rescue workers. In recent years, the exposure of mine rescue teams to hazardous situations has been changing. However, it is vital that members of the teams have the capability and proper protection to immediately respond to a wide range of hazardous situations. Currently, there are no minimum requirements, best practice documents, or nationally recognized consensus standards for protective clothing used by mine rescue teams in the United States (U.S.). The following review provides a summary of potential issues that can be addressed by rescue teams and industry to improve potential exposures to rescue team members should a disaster situation occur. However, the continued trending in the mining industry toward non-exposure to potential hazards for rescue workers should continue to be the primary goal. To assist in continuing this trend, the mining industry and regulatory agencies have been more restrictive by requiring additional post disaster information regarding atmospheric conditions and other hazards before exposing rescue workers and others in the aftermath of a mine disaster. In light of some of the more recent mine rescuer fatalities such as the Crandall Canyon Mine and Jim Walters Resources in the past years, the direction of reducing exposure is preferred. This review provides a historical perspective on ensembles used during mine rescue operations and summarizes environmental hazards, critical elements of mine rescue ensembles, and key problems with these elements. This study also identifies domains for improved mine rescue ensembles. Furthermore, field observations from several coal mine rescue teams were added to provide the information on the currently used mine rescue ensembles in the U.S. PMID:27065231

QuantFusion: Novel Unified Methodology for Enhanced Coverage and Precision in Quantifying Global Proteomic Changes in Whole Tissues.

PubMed

Gunawardena, Harsha P; O'Brien, Jonathon; Wrobel, John A; Xie, Ling; Davies, Sherri R; Li, Shunqiang; Ellis, Matthew J; Qaqish, Bahjat F; Chen, Xian

2016-02-01

Single quantitative platforms such as label-based or label-free quantitation (LFQ) present compromises in accuracy, precision, protein sequence coverage, and speed of quantifiable proteomic measurements. To maximize the quantitative precision and the number of quantifiable proteins or the quantifiable coverage of tissue proteomes, we have developed a unified approach, termed QuantFusion, that combines the quantitative ratios of all peptides measured by both LFQ and label-based methodologies. Here, we demonstrate the use of QuantFusion in determining the proteins differentially expressed in a pair of patient-derived tumor xenografts (PDXs) representing two major breast cancer (BC) subtypes, basal and luminal. Label-based in-spectra quantitative peptides derived from amino acid-coded tagging (AACT, also known as SILAC) of a non-malignant mammary cell line were uniformly added to each xenograft with a constant predefined ratio, from which Ratio-of-Ratio estimates were obtained for the label-free peptides paired with AACT peptides in each PDX tumor. A mixed model statistical analysis was used to determine global differential protein expression by combining complementary quantifiable peptide ratios measured by LFQ and Ratio-of-Ratios, respectively. With minimum number of replicates required for obtaining the statistically significant ratios, QuantFusion uses the distinct mechanisms to "rescue" the missing data inherent to both LFQ and label-based quantitation. Combined quantifiable peptide data from both quantitative schemes increased the overall number of peptide level measurements and protein level estimates. In our analysis of the PDX tumor proteomes, QuantFusion increased the number of distinct peptide ratios by 65%, representing differentially expressed proteins between the BC subtypes. This quantifiable coverage improvement, in turn, not only increased the number of measurable protein fold-changes by 8% but also increased the average precision of quantitative estimates by 181% so that some BC subtypically expressed proteins were rescued by QuantFusion. Thus, incorporating data from multiple quantitative approaches while accounting for measurement variability at both the peptide and global protein levels make QuantFusion unique for obtaining increased coverage and quantitative precision for tissue proteomes. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Metal Binding by the D,DX35E Motif of Human Immunodeficiency Virus Type 1 Integrase: Selective Rescue of Cys Substitutions by Mn2+ In Vitro

PubMed Central

Gao, Kui; Wong, Steven; Bushman, Frederic

2004-01-01

The D,DX35E motif characteristic of retroviral integrase enzymes (INs) is expected to bind the required metal cofactors (Mg2+ or Mn2+), but direct evidence for a catalytic role has been lacking. Here we used a metal rescue strategy to investigate metal binding. We established conditions for analysis of an activity of IN, disintegration, in both Mg2+ and Mn2+, and tested IN mutants with cysteine substitutions in each acidic residue of the D,DX35E motif. Mn2+ but not Mg2+ can bind tightly to Cys, so if metal binding at the acidic residues is mechanistically important, it is expected that the Cys-substituted enzymes would be active in the presence of Mn2+ only. Of the three acidic residues, a strong metal rescue effect was obtained for D116C, a weaker rescue was seen for D64C, and no rescue was seen with E152C. Modest rescue could also be detected for D116C in normal integration in vitro. Comparison to Ser and Ala substitutions at D116 established that the rescue was selective for Cys. Further studies of the response to pH suggest that the metal cofactor may stabilize the deprotonated nucleophile active in catalysis, and studies of the response to NaCl titrations disclose an additional role for the metal cofactor in stabilizing the IN-DNA complex. PMID:15194746

The chromokinesin Kid is required for maintenance of proper metaphase spindle size.

PubMed

Tokai-Nishizumi, Noriko; Ohsugi, Miho; Suzuki, Emiko; Yamamoto, Tadashi

2005-11-01

The human chromokinesin Kid/kinesin-10, a plus end-directed microtubule (MT)-based motor with both microtubule- and DNA-binding domains, is required for proper chromosome alignment at the metaphase plate. Here, we performed RNA interference experiments to deplete endogenous Kid from HeLa cells and confirmed defects in metaphase chromosome arm alignment in Kid-depleted cells. In addition, we noted a shortening of the spindle length, resulting in a pole-to-pole distance only 80% of wild type. The spindle microtubule-bundles with which Kid normally colocalize became less robust. Rescue of the two Kid deficiency phenotypes-imprecise chromosome alignment at metaphase and shortened spindles- exhibited distinct requirements. Mutants lacking either the DNA-binding domain or the MT motor ATPase failed to rescue the former defect, whereas rescue of the shortened spindle phenotype required neither activity. Kid also exhibits microtubule bundling activity in vitro, and rescue of the shortened spindle phenotype and the bundling activity displayed similar domain requirements, except that rescue required a coiled-coil domain not needed for bundling. These results suggest that distinct from its role in chromosome movement, Kid contributes to spindle morphogenesis by mediating spindle microtubules stabilization.

The Chromokinesin Kid Is Required for Maintenance of Proper Metaphase Spindle SizeD⃞

PubMed Central

Tokai-Nishizumi, Noriko; Ohsugi, Miho; Suzuki, Emiko; Yamamoto, Tadashi

2005-01-01

The human chromokinesin Kid/kinesin-10, a plus end-directed microtubule (MT)-based motor with both microtubule- and DNA-binding domains, is required for proper chromosome alignment at the metaphase plate. Here, we performed RNA interference experiments to deplete endogenous Kid from HeLa cells and confirmed defects in metaphase chromosome arm alignment in Kid-depleted cells. In addition, we noted a shortening of the spindle length, resulting in a pole-to-pole distance only 80% of wild type. The spindle microtubule-bundles with which Kid normally colocalize became less robust. Rescue of the two Kid deficiency phenotypes—imprecise chromosome alignment at metaphase and shortened spindles— exhibited distinct requirements. Mutants lacking either the DNA-binding domain or the MT motor ATPase failed to rescue the former defect, whereas rescue of the shortened spindle phenotype required neither activity. Kid also exhibits microtubule bundling activity in vitro, and rescue of the shortened spindle phenotype and the bundling activity displayed similar domain requirements, except that rescue required a coiled-coil domain not needed for bundling. These results suggest that distinct from its role in chromosome movement, Kid contributes to spindle morphogenesis by mediating spindle microtubules stabilization. PMID:16176979

Molecular mechanism of action of pharmacoperone rescue of misrouted GPCR mutants: the GnRH receptor.

PubMed

Janovick, Jo Ann; Patny, Akshay; Mosley, Ralph; Goulet, Mark T; Altman, Michael D; Rush, Thomas S; Cornea, Anda; Conn, P Michael

2009-02-01

The human GnRH receptor (hGnRHR), a G protein-coupled receptor, is a useful model for studying pharmacological chaperones (pharmacoperones), drugs that rescue misfolded and misrouted protein mutants and restore them to function. This technique forms the basis of a therapeutic approach of rescuing mutants associated with human disease and restoring them to function. The present study relies on computational modeling, followed by site-directed mutagenesis, assessment of ligand binding, effector activation, and confocal microscopy. Our results show that two different chemical classes of pharmacoperones act to stabilize hGnRHR mutants by bridging residues D(98) and K(121). This ligand-mediated bridge serves as a surrogate for a naturally occurring and highly conserved salt bridge (E(90)-K(121)) that stabilizes the relation between transmembranes 2 and 3, which is required for passage of the receptor through the cellular quality control system and to the plasma membrane. Our model was used to reveal important pharmacophoric features, and then identify a novel chemical ligand, which was able to rescue a D(98) mutant of the hGnRHR that could not be rescued as effectively by previously known pharmacoperones.

Recovery of infectious type Asia1 foot-and-mouth disease virus from suckling mice directly inoculated with an RNA polymerase I/II-driven unidirectional transcription plasmid.

PubMed

Lian, Kaiqi; Yang, Fan; Zhu, Zixiang; Cao, Weijun; Jin, Ye; Li, Dan; Zhang, Keshan; Guo, Jianhong; Zheng, Haixue; Liu, Xiangtao

2015-10-02

We developed an RNA polymerase (pol) I- and II-driven plasmid-based reverse genetics system to rescue infectious foot-and-mouth disease virus (FMDV) from cloned cDNA. In this plasmid-based transfection, the full-length viral cDNA was flanked by hammerhead ribozyme (HamRz) and hepatitis delta ribozyme (HdvRz) sequences, which were arranged downstream of the two promoters (cytomegalovirus (CMV) and pol I promoter) and upstream of the terminators and polyadenylation signal, respectively. The utility of this method was demonstrated by the recovery of FMDV Asia1 HN/CHA/06 in BHK-21 cells transfected with cDNA plasmids. Furthermore, infectious FMDV Asia1 HN/CHA/06 could be rescued from suckling mice directly inoculated with cDNA plasmids. Thus, this reverse genetics system can be applied to fundamental research and vaccine studies, most notably to rescue those viruses for which there is currently an absence of a suitable cell culture system. Copyright © 2015 Elsevier B.V. All rights reserved.

Wireless and Low-Weight Technologies: Advanced Medical Assistance During a Cave Rescue: A Case Report.

PubMed

Petrucci, Emiliano; Pizzi, Barbara; Scimia, Paolo; Conti, Giuseppe; Di Carlo, Stefano; Santini, Antonella; Fusco, Pierfrancesco

2018-06-01

Trauma care in cave rescue is a unique situation that requires an advanced and organized approach with medical and technical assistance because of the extreme environmental conditions and logistical factors. In caving accidents, the most common injuries involve lower limbs. We describe an advanced medical rescue performed by the Italian Corpo Nazionale del Soccorso Alpino e Speleologico, in which extended focused assessment with sonography for trauma and an ultrasound-guided adductor canal block were performed on a patient with a knee distortion directly in the cave. The rescue team inside the cave shared data on patient monitoring and the ultrasound scanning in real time with rescuers at the entrance, using a video conference powered by the new Ermes system. The use of handheld, battery-powered, low-weight, multiparametric monitors, ultrasound machines, and digital data transmission systems could ensure complete medical assistance in harsh environmental conditions such as those found in a cave. Copyright © 2018 Wilderness Medical Society. Published by Elsevier Inc. All rights reserved.

Detection and Identification of Multiple Stationary Human Targets Via Bio-Radar Based on the Cross-Correlation Method

PubMed Central

Zhang, Yang; Chen, Fuming; Xue, Huijun; Li, Zhao; An, Qiang; Wang, Jianqi; Zhang, Yang

2016-01-01

Ultra-wideband (UWB) radar has been widely used for detecting human physiological signals (respiration, movement, etc.) in the fields of rescue, security, and medicine owing to its high penetrability and range resolution. In these applications, especially in rescue after disaster (earthquake, collapse, mine accident, etc.), the presence, number, and location of the trapped victims to be detected and rescued are the key issues of concern. Ample research has been done on the first issue, whereas the identification and localization of multi-targets remains a challenge. False positive and negative identification results are two common problems associated with the detection of multiple stationary human targets. This is mainly because the energy of the signal reflected from the target close to the receiving antenna is considerably stronger than those of the targets at further range, often leading to missing or false recognition if the identification method is based on the energy of the respiratory signal. Therefore, a novel method based on cross-correlation is proposed in this paper that is based on the relativity and periodicity of the signals, rather than on the energy. The validity of this method is confirmed through experiments using different scenarios; the results indicate a discernible improvement in the detection precision and identification of the multiple stationary targets. PMID:27801795

Detection and Identification of Multiple Stationary Human Targets Via Bio-Radar Based on the Cross-Correlation Method.

PubMed

Zhang, Yang; Chen, Fuming; Xue, Huijun; Li, Zhao; An, Qiang; Wang, Jianqi; Zhang, Yang

2016-10-27

Ultra-wideband (UWB) radar has been widely used for detecting human physiological signals (respiration, movement, etc.) in the fields of rescue, security, and medicine owing to its high penetrability and range resolution. In these applications, especially in rescue after disaster (earthquake, collapse, mine accident, etc.), the presence, number, and location of the trapped victims to be detected and rescued are the key issues of concern. Ample research has been done on the first issue, whereas the identification and localization of multi-targets remains a challenge. False positive and negative identification results are two common problems associated with the detection of multiple stationary human targets. This is mainly because the energy of the signal reflected from the target close to the receiving antenna is considerably stronger than those of the targets at further range, often leading to missing or false recognition if the identification method is based on the energy of the respiratory signal. Therefore, a novel method based on cross-correlation is proposed in this paper that is based on the relativity and periodicity of the signals, rather than on the energy. The validity of this method is confirmed through experiments using different scenarios; the results indicate a discernible improvement in the detection precision and identification of the multiple stationary targets.

Reverse Genetics of Newcastle Disease Virus.

PubMed

Cardenas-Garcia, Stivalis; Afonso, Claudio L

2017-01-01

Reverse genetics allows for the generation of recombinant viruses or vectors used in functional studies, vaccine development, and gene therapy. This technique enables genetic manipulation and cloning of viral genomes, gene mutation through site-directed mutagenesis, along with gene insertion or deletion, among other studies. An in vitro infection-based system including the highly attenuated vaccinia virus Ankara strain expressing the T7 RNA polymerase from bacteriophage T7, with co-transfection of three helper plasmids and a full-length cDNA plasmid, was successfully developed to rescue genetically modified Newcastle disease viruses in 1999. In this chapter, the materials and the methods involved in rescuing Newcastle disease virus (NDV) from cDNA, utilizing site-directed mutagenesis and gene replacement techniques, are described in detail.

Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.

PubMed

Pate, Kira T; Stringari, Chiara; Sprowl-Tanio, Stephanie; Wang, Kehui; TeSlaa, Tara; Hoverter, Nate P; McQuade, Miriam M; Garner, Chad; Digman, Michelle A; Teitell, Michael A; Edwards, Robert A; Gratton, Enrico; Waterman, Marian L

2014-07-01

Much of the mechanism by which Wnt signaling drives proliferation during oncogenesis is attributed to its regulation of the cell cycle. Here, we show how Wnt/β-catenin signaling directs another hallmark of tumorigenesis, namely Warburg metabolism. Using biochemical assays and fluorescence lifetime imaging microscopy (FLIM) to probe metabolism in vitro and in living tumors, we observe that interference with Wnt signaling in colon cancer cells reduces glycolytic metabolism and results in small, poorly perfused tumors. We identify pyruvate dehydrogenase kinase 1 (PDK1) as an important direct target within a larger gene program for metabolism. PDK1 inhibits pyruvate flux to mitochondrial respiration and a rescue of its expression in Wnt-inhibited cancer cells rescues glycolysis as well as vessel growth in the tumor microenvironment. Thus, we identify an important mechanism by which Wnt-driven Warburg metabolism directs the use of glucose for cancer cell proliferation and links it to vessel delivery of oxygen and nutrients. © 2014 The Authors.

Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer

PubMed Central

Pate, Kira T; Stringari, Chiara; Sprowl-Tanio, Stephanie; Wang, Kehui; TeSlaa, Tara; Hoverter, Nate P; McQuade, Miriam M; Garner, Chad; Digman, Michelle A; Teitell, Michael A; Edwards, Robert A; Gratton, Enrico; Waterman, Marian L

2014-01-01

Much of the mechanism by which Wnt signaling drives proliferation during oncogenesis is attributed to its regulation of the cell cycle. Here, we show how Wnt/β-catenin signaling directs another hallmark of tumorigenesis, namely Warburg metabolism. Using biochemical assays and fluorescence lifetime imaging microscopy (FLIM) to probe metabolism in vitro and in living tumors, we observe that interference with Wnt signaling in colon cancer cells reduces glycolytic metabolism and results in small, poorly perfused tumors. We identify pyruvate dehydrogenase kinase 1 (PDK1) as an important direct target within a larger gene program for metabolism. PDK1 inhibits pyruvate flux to mitochondrial respiration and a rescue of its expression in Wnt-inhibited cancer cells rescues glycolysis as well as vessel growth in the tumor microenvironment. Thus, we identify an important mechanism by which Wnt-driven Warburg metabolism directs the use of glucose for cancer cell proliferation and links it to vessel delivery of oxygen and nutrients. PMID:24825347

Combined Angio-Seal™ and stenting rescue treatment in a case of iatrogenic common carotid artery dissection during direct puncture for ruptured intracranial aneurysm embolization: a technical note.

PubMed

Iosif, Christina; Clarençon, Frédéric; Di Maria, Federico; Law-Ye, Bruno; Le Jean, Lise; Capelle, Laurent; Chiras, Jacques; Sourour, Nader

2013-05-01

Direct puncture may offer an alternative access for embolization of intracranial aneurysms in patients presenting with tortuous vessels. Nevertheless, major complications such as compressive hematoma and arterial dissection can occur with this technique. A tight common carotid artery (CCA) dissection was seen secondary to direct puncture in a 72-year-old patient who presented with a ruptured anterior communicating artery (ACom) aneurysm. After regular coiling of the aneurysm and using a femoral approach, an Angio-Seal™ device (St Jude Medical, Saint Paul, MN, USA) was placed and a carotid wallstent (Stryker Neurovascular, Fremont, CA, USA) successfully deployed at the dissected CCA, which was followed by good clinical and angiographic outcomes. A combined rescue technique combining Angio-Seal insertion and stent deployment was safe and effective for managing iatrogenic carotid artery dissection. Copyright © 2013. Published by Elsevier Masson SAS.

Bispectral Index Monitoring during Anesthesiologist-Directed Propofol and Remifentanil Sedation for Endoscopic Submucosal Dissection: A Prospective Randomized Controlled Trial

PubMed Central

Park, Woo Young; Shin, Yang-Sik; Lee, Sang Kil; Kim, So Yeon; Lee, Tai Kyung

2014-01-01

Purpose Endoscopic submucosal dissection (ESD) is a technically difficult and lengthy procedure requiring optimal depth of sedation. The bispectral index (BIS) monitor is a non-invasive tool that objectively evaluates the depth of sedation. The purpose of this prospective randomized controlled trial was to evaluate whether BIS guided sedation with propofol and remifentanil could reduce the number of patients requiring rescue propofol, and thus reduce the incidence of sedation- and/or procedure-related complications. Materials and Methods A total of 180 patients who underwent the ESD procedure for gastric adenoma or early gastric cancer were randomized to two groups. The control group (n=90) was monitored by the Modified Observer's Assessment of Alertness and Sedation scale and the BIS group (n=90) was monitored using BIS. The total doses of propofol and remifentanil, the need for rescue propofol, and the rates of complications were recorded. Results The number of patients who needed rescue propofol during the procedure was significantly higher in the control group than the BIS group (47.8% vs. 30.0%, p=0.014). There were no significant differences in the incidence of sedation- and/or procedure-related complications. Conclusion BIS-guided propofol infusion combined with remifentanil reduced the number of patients requiring rescue propofol in ESD procedures. However, this finding did not lead to clinical benefits and thus BIS monitoring is of limited use during anesthesiologist-directed sedation. PMID:25048506

Rescue of newborn ants by older Cataglyphis cursor adult workers.

PubMed

Nowbahari, Elise; Amirault, Céline; Hollis, Karen L

2016-05-01

Cataglyphis cursor worker ants are capable of highly sophisticated rescue behaviour in which individuals are able to identify what has trapped a nestmate and to direct their behaviour towards that obstacle. Nonetheless, rescue behaviour is constrained by workers' subcaste: whereas foragers, the oldest workers, are able both to give and to receive the most help, the youngest workers, inactives, neither give nor receive any help whatsoever; nurses give and receive intermediate levels of aid, reflecting their intermediate age. Such differences in rescue behaviour across subcastes suggest that age and experience play a critical role. In this species, as in many others in which a sensitive period for nestmate recognition exists, newly enclosed ants, called callows, are adopted by ants belonging not only to different colonies but also to different species; foreign callows receive nearly the same special care provided to resident newborns. Because callows are younger than inactives, which are incapable of soliciting rescue, we wondered whether entrapped callows would receive such aid. In the present study, we artificially ensnared individual callows from their own colony (homocolonial), from a different colony (heterocolonial), and from a different species (heterospecific), and tested each one with groups of five potential C. cursor rescuers, either all foragers or all nurses. Our results show that all three types of callows are able to elicit rescue behaviour from both foragers and nurses. Nonetheless, nurse rescuers are better able to discriminate between the three types of callow victims than are foragers.

Missense Mutations Allow a Sequence-Blind Mutant of SpoIIIE to Successfully Translocate Chromosomes during Sporulation.

PubMed

Bose, Baundauna; Reed, Sydney E; Besprozvannaya, Marina; Burton, Briana M

2016-01-01

SpoIIIE directionally pumps DNA across membranes during Bacillus subtilis sporulation and vegetative growth. The sequence-reading domain (γ domain) is required for directional DNA transport, and its deletion severely impairs sporulation. We selected suppressors of the spoIIIEΔγ sporulation defect. Unexpectedly, many suppressors were intragenic missense mutants, and some restore sporulation to near-wild-type levels. The mutant proteins are likely not more abundant, faster at translocating DNA, or sequence-sensitive, and rescue does not involve the SpoIIIE homolog SftA. Some mutants behave differently when co-expressed with spoIIIEΔγ, consistent with the idea that some, but not all, variants may form mixed oligomers. In full-length spoIIIE, these mutations do not affect sporulation, and yet the corresponding residues are rarely found in other SpoIIIE/FtsK family members. The suppressors do not rescue chromosome translocation defects during vegetative growth, indicating that the role of the γ domain cannot be fully replaced by these mutations. We present two models consistent with our findings: that the suppressors commit to transport in one arbitrarily-determined direction or delay spore development. It is surprising that missense mutations somehow rescue loss of an entire domain with a complex function, and this raises new questions about the mechanism by which SpoIIIE pumps DNA and the roles SpoIIIE plays in vivo.

Palmitoylation of LIM Kinase-1 ensures spine-specific actin polymerization and morphological plasticity

PubMed Central

George, Joju; Soares, Cary; Montersino, Audrey; Beique, Jean-Claude; Thomas, Gareth M

2015-01-01

Precise regulation of the dendritic spine actin cytoskeleton is critical for neurodevelopment and neuronal plasticity, but how neurons spatially control actin dynamics is not well defined. Here, we identify direct palmitoylation of the actin regulator LIM kinase-1 (LIMK1) as a novel mechanism to control spine-specific actin dynamics. A conserved palmitoyl-motif is necessary and sufficient to target LIMK1 to spines and to anchor LIMK1 in spines. ShRNA knockdown/rescue experiments reveal that LIMK1 palmitoylation is essential for normal spine actin polymerization, for spine-specific structural plasticity and for long-term spine stability. Palmitoylation is critical for LIMK1 function because this modification not only controls LIMK1 targeting, but is also essential for LIMK1 activation by its membrane-localized upstream activator PAK. These novel roles for palmitoylation in the spatial control of actin dynamics and kinase signaling provide new insights into structural plasticity mechanisms and strengthen links between dendritic spine impairments and neuropathological conditions. DOI: http://dx.doi.org/10.7554/eLife.06327.001 PMID:25884247

Nociceptive Sensory Fibers Drive Interleukin-23 Production from CD301b+ Dermal Dendritic Cells and Drive Protective Cutaneous Immunity.

PubMed

Kashem, Sakeen W; Riedl, Maureen S; Yao, Chen; Honda, Christopher N; Vulchanova, Lucy; Kaplan, Daniel H

2015-09-15

Innate resistance to Candida albicans in mucosal tissues requires the production of interleukin-17A (IL-17A) by tissue-resident cells early during infection, but the mechanism of cytokine production has not been precisely defined. In the skin, we found that dermal γδ T cells were the dominant source of IL-17A during C. albicans infection and were required for pathogen resistance. Induction of IL-17A from dermal γδ T cells and resistance to C. albicans required IL-23 production from CD301b(+) dermal dendritic cells (dDCs). In addition, we found that sensory neurons were directly activated by C. albicans. Ablation of sensory neurons increased susceptibility to C. albicans infection, which could be rescued by exogenous addition of the neuropeptide CGRP. These data define a model in which nociceptive pathways in the skin drive production of IL-23 by CD301b(+) dDCs resulting in IL-17A production from γδ T cells and resistance to cutaneous candidiasis. Copyright © 2015 Elsevier Inc. All rights reserved.

Age-Related Decline in Primary CD8+ T Cell Responses Is Associated with the Development of Senescence in Virtual Memory CD8+ T Cells.

PubMed

Quinn, Kylie M; Fox, Annette; Harland, Kim L; Russ, Brendan E; Li, Jasmine; Nguyen, Thi H O; Loh, Liyen; Olshanksy, Moshe; Naeem, Haroon; Tsyganov, Kirill; Wiede, Florian; Webster, Rosela; Blyth, Chantelle; Sng, Xavier Y X; Tiganis, Tony; Powell, David; Doherty, Peter C; Turner, Stephen J; Kedzierska, Katherine; La Gruta, Nicole L

2018-06-19

Age-associated decreases in primary CD8 + T cell responses occur, in part, due to direct effects on naive CD8 + T cells to reduce intrinsic functionality, but the precise nature of this defect remains undefined. Aging also causes accumulation of antigen-naive but semi-differentiated "virtual memory" (T VM ) cells, but their contribution to age-related functional decline is unclear. Here, we show that T VM cells are poorly proliferative in aged mice and humans, despite being highly proliferative in young individuals, while conventional naive T cells (T N cells) retain proliferative capacity in both aged mice and humans. Adoptive transfer experiments in mice illustrated that naive CD8 T cells can acquire a proliferative defect imposed by the aged environment but age-related proliferative dysfunction could not be rescued by a young environment. Molecular analyses demonstrate that aged T VM cells exhibit a profile consistent with senescence, marking an observation of senescence in an antigenically naive T cell population. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

KSC-08pd3552

NASA Image and Video Library

2008-11-06

CAPE CANAVERAL, Fla. – At the Shuttle Landing Facility at NASA's Kennedy Space Center in Florida, the U.S. Navy's Blue Angel pilots and support team are greeted by (from right) Special Operations Director Joe Dowdy, External Relations Director Lisa Malone, External Relations Acting Deputy Director Cheryl Hurst and Pam Steele, chief of the Public Services Division at Kennedy. The Blue Angels will perform at the Kennedy Space Center Visitor Complex Space and Air Show Nov. 8-9. The Navy's elite flight demonstration squadron will take to the skies in military aircraft demonstrations by the F-16 Fighting Falcon and F/A-18 Super Hornet jets for the second annual Space & Air Show at Kennedy. This year’s show brings together the best in military aircraft, coupled with precision pilots and veteran astronauts to celebrate spaceflight and aviation. The event includes military aircraft demonstrations by the F-16 Fighting Falcon and a water rescue demonstration by the 920th Rescue Wing. Photo credit: NASA/Kim Shiflett

Self-rescue strategies for EVA crewmembers equipped with the SAFER backpack

NASA Technical Reports Server (NTRS)

Williams, Trevor; Baughman, David

1994-01-01

An extravehicular astronaut who becomes separated from a space station has three options available: grappling the station immediately by means of a 'shepherd's crook' device; rescue by either a second crewmember flying an MMU or a robotic-controlled MMU; or self-rescue by means of a propulsive system. The first option requires very fast response by a tumbling astronaut; the second requires constant availability of an MMU, as well as a rendezvous procedure thousands of feet from the station. This paper will consider the third option, propulsive self-rescue. In particular, the capability of the new Simplified Aid for EVA Rescue (SAFER) propulsive backpack, which is to be tested on STS-64 in Sep. 1994, will be studied. This system possesses an attitude hold function that can automatically detumble an astronaut after separation. On-orbit tests of candidate self-rescue systems have demonstrated the need for such a feature. SAFER has a total delta(v) capability of about 10 fps, to cover both rotations and translations, compared with a possible separation rate of 2.5 fps. But the delta(v) required for self-rescue is critically dependent on the delay before return can be initiated, as a consequence of orbital effects. A very important practical question is then whether the total delta(v) of SAFER is adequate to perform self-rescue for worst case values of separation speed, time to detumble, and time for the astronaut to visually acquire the station. This paper shows that SAFER does indeed have sufficient propellant to carry out self-rescue in all realistic separation cases, as well as in cases which are considerably more severe than anything likely to be encountered in practice. The return trajectories and total delta(v)'s discussed are obtained by means of an 'inertial line-of-sight targeting' scheme, derived in the paper, which allows orbital effects to be corrected by making use of the visual information available to the pilot, namely the line-of-sight direction to the station relative to the stars.

Increased Risk Proneness or Social Withdrawal? The Effects of Shortened Life Expectancy on the Expression of Rescue Behavior in Workers of the ant Formica cinerea (Hymenoptera: Formicidae).

PubMed

Miler, Krzysztof; Symonowicz, Beata; Godzińska, Ewa J

2017-01-01

In social insects behavioral consequences of shortened life expectancy include, among others, increased risk proneness and social withdrawal. We investigated the impact of experimental shortening of life expectancy of foragers of the ant Formica cinerea achieved by their exposure to carbon dioxide on the expression of rescue behavior, risky pro-social behavior, tested by means of two bioassays during which a single worker (rescuer) was confronted with a nestmate (victim) attacked by a predator (antlion larva capture bioassay) or immobilized by an artificial snare (entrapment bioassay). Efficacy of carbon dioxide poisoning in shortening life expectancy was confirmed by the analysis of ant mortality. Rescue behavior observed during behavioral tests involved digging around the victim, transport of the sand covering the victim, pulling the limbs/antennae/mandibles of the victim, direct attack on the antlion (in antlion larva capture tests), and snare biting (in entrapment tests). The rate of occurrence of rescue behavior was lower in ants with shortened life expectancy, but that effect was significant only in the case of the entrapment bioassay. Similarly, only in the case of the entrapment bioassay ants with shortened life expectancy displayed rescue behavior after a longer latency and devoted less time to that behavior than ants from the control groups. Our results demonstrated that in ant workers shortened life expectancy may lead to reduced propensity for rescue behavior, most probably as an element of the social withdrawal syndrome that had already been described in several studies on behavior of moribund ants and honeybees.

A Module for Automatic Dock and Detumble (MADD) for orbital rescue operations

NASA Technical Reports Server (NTRS)

Snow, W. R.; Kunciw, B. G.; Kaplan, M. H.

1973-01-01

The module for automatic dock and detumble (MADD) is an automated device for bringing a passive, tumbling space base under control in an orbital rescue situation. The conceptual design of such a device resulted from a consideration of tumbling motion analyses and mission constraints. Specific topics of investigation include orbit and attitude dynamics and detumble profiles. Position and attitude control systems for the various phases of operation were developed. Dynamic motion of a passive vehicle with MADD attached is considered as an example application and to determine control requirements. Since time is a critical factor in rescue operations, it is essential to execute the detumbling maneuver in a minimum of time. Optimization of the MADD thrusting sequence has also been investigated. Results indicate the control torque must be directed opposite to the angular momentum vector for the assumption used here.

SERCA directs cell migration and branching across species and germ layers

PubMed Central

Lansdale, Nick; Navarro, Sonia; Truong, Thai V.; Bower, Dan J.; Featherstone, Neil C.; Connell, Marilyn G.; Al Alam, Denise; Frey, Mark R.; Trinh, Le A.; Fernandez, G. Esteban; Warburton, David; Fraser, Scott E.; Bennett, Daimark; Jesudason, Edwin C.

2017-01-01

ABSTRACT Branching morphogenesis underlies organogenesis in vertebrates and invertebrates, yet is incompletely understood. Here, we show that the sarco-endoplasmic reticulum Ca2+ reuptake pump (SERCA) directs budding across germ layers and species. Clonal knockdown demonstrated a cell-autonomous role for SERCA in Drosophila air sac budding. Live imaging of Drosophila tracheogenesis revealed elevated Ca2+ levels in migratory tip cells as they form branches. SERCA blockade abolished this Ca2+ differential, aborting both cell migration and new branching. Activating protein kinase C (PKC) rescued Ca2+ in tip cells and restored cell migration and branching. Likewise, inhibiting SERCA abolished mammalian epithelial budding, PKC activation rescued budding, while morphogens did not. Mesoderm (zebrafish angiogenesis) and ectoderm (Drosophila nervous system) behaved similarly, suggesting a conserved requirement for cell-autonomous Ca2+ signaling, established by SERCA, in iterative budding. PMID:28821490

Power sources for search and rescue 406 MHz beacons

NASA Technical Reports Server (NTRS)

Attia, Alan I.; Perrone, David E.

1987-01-01

The results of a study directed at the selection of a commercially available, safe, low cost, light weight and long storage life battery for search and rescue (Sarsat) 406 MHz emergency beacons are presented. In the course of this work, five electrochemical systems (lithium-manganese dioxide, lithium-carbon monofluoride, lithium-silver vanadium oxide, alkaline cells, and cadmium-mercuric oxide) were selected for limited experimental studies to determine their suitability for this application. Two safe, commercially available batteries (lithium-manganese dioxide and lithium-carbon monofluoride) which meet the near term requirements and several alternatives for the long term were identified.

Dynamics of omnidirectional unmanned rescue vehicle with mecanum wheels

NASA Astrophysics Data System (ADS)

Typiak, Andrzej; Łopatka, Marian Janusz; Rykała, Łukasz; Kijek, Magdalena

2018-01-01

The work presents the dynamic equations of motion of a unmanned six-wheeled vehicle with mecanum wheels for rescue applications derived with the of Lagrange equations of the second kind with multipliers. Analysed vehicle through using mecanum wheels has three degrees of freedom and can move on a flat ground in any direction with any configuration of platform's frame. In order to derive dynamic equations of motion of mentioned object, kinetic potential of the system and generalized forces affecting the system are determined. The results of a solution of inverse dynamics problem are also published.

Extracellular matrix protein 1, a direct targeting molecule of parathyroid hormone-related peptide, negatively regulates chondrogenesis and endochondral ossification via associating with progranulin growth factor.

PubMed

Kong, Li; Zhao, Yun-Peng; Tian, Qing-Yun; Feng, Jian-Quan; Kobayashi, Tatsuya; Merregaert, Joseph; Liu, Chuan-Ju

2016-08-01

Chondrogenesis and endochondral ossification are precisely controlled by cellular interactions with surrounding matrix proteins and growth factors that mediate cellular signaling pathways. Here, we report that extracellular matrix protein 1 (ECM1) is a previously unrecognized regulator of chondrogenesis. ECM1 is induced in the course of chondrogenesis and its expression in chondrocytes strictly depends on parathyroid hormone-related peptide (PTHrP) signaling pathway. Overexpression of ECM1 suppresses, whereas suppression of ECM1 enhances, chondrocyte differentiation and hypertrophy in vitro and ex vivo In addition, target transgene of ECM1 in chondrocytes or osteoblasts in mice leads to striking defects in cartilage development and endochondral bone formation. Of importance, ECM1 seems to be critical for PTHrP action in chondrogenesis, as blockage of ECM1 nearly abolishes PTHrP regulation of chondrocyte hypertrophy, and overexpression of ECM1 rescues disorganized growth plates of PTHrP-null mice. Furthermore, ECM1 and progranulin chondrogenic growth factor constitute an interaction network and act in concert in the regulation of chondrogenesis.-Kong, L., Zhao, Y.-P., Tian, Q.-Y., Feng, J.-Q., Kobayashi, T., Merregaert, J., Liu, C.-J. Extracellular matrix protein 1, a direct targeting molecule of parathyroid hormone-related peptide, negatively regulates chondrogenesis and endochondral ossification via associating with progranulin growth factor. © FASEB.

Extracellular matrix protein 1, a direct targeting molecule of parathyroid hormone–related peptide, negatively regulates chondrogenesis and endochondral ossification via associating with progranulin growth factor

PubMed Central

Kong, Li; Zhao, Yun-Peng; Tian, Qing-Yun; Feng, Jian-Quan; Kobayashi, Tatsuya; Merregaert, Joseph; Liu, Chuan-Ju

2016-01-01

Chondrogenesis and endochondral ossification are precisely controlled by cellular interactions with surrounding matrix proteins and growth factors that mediate cellular signaling pathways. Here, we report that extracellular matrix protein 1 (ECM1) is a previously unrecognized regulator of chondrogenesis. ECM1 is induced in the course of chondrogenesis and its expression in chondrocytes strictly depends on parathyroid hormone–related peptide (PTHrP) signaling pathway. Overexpression of ECM1 suppresses, whereas suppression of ECM1 enhances, chondrocyte differentiation and hypertrophy in vitro and ex vivo. In addition, target transgene of ECM1 in chondrocytes or osteoblasts in mice leads to striking defects in cartilage development and endochondral bone formation. Of importance, ECM1 seems to be critical for PTHrP action in chondrogenesis, as blockage of ECM1 nearly abolishes PTHrP regulation of chondrocyte hypertrophy, and overexpression of ECM1 rescues disorganized growth plates of PTHrP-null mice. Furthermore, ECM1 and progranulin chondrogenic growth factor constitute an interaction network and act in concert in the regulation of chondrogenesis.—Kong, L., Zhao, Y.-P., Tian, Q.-Y., Feng, J.-Q., Kobayashi, T., Merregaert, J., Liu, C.-J. Extracellular matrix protein 1, a direct targeting molecule of parathyroid hormone–related peptide, negatively regulates chondrogenesis and endochondral ossification via associating with progranulin growth factor. PMID:27075243

β-Secretase inhibitor GRL-8234 rescues age-related cognitive decline in APP transgenic mice

PubMed Central

Chang, Wan-Pin; Huang, Xiangping; Downs, Deborah; Cirrito, John R.; Koelsch, Gerald; Holtzman, David M.; Ghosh, Arun K.; Tang, Jordan

2011-01-01

Alzheimer disease is intimately linked to an excess amount of amyloid-β (Aβ) in the brain. Thus, therapeutic inhibition of Aβ production is an attractive clinical approach to treat this disease. Here we provide the first direct experimental evidence that the treatment of Tg2576 transgenic mice with an inhibitor of β-secretase, GRL-8234, rescues the age-related cognitive decline. We demonstrated that the injected GRL-8234 effectively enters the brain and rapidly decreases soluble Aβ in the brain of Tg2576 mice. The rescue of cognition, which was observed only after long-term inhibitor treatment ranging from 5 to 7.5 mo, was associated with a decrease of brain amyloid-β plaque load. We also found no accumulation of amyloid-β precursor protein after several months of inhibitor treatment. These observations substantiate the idea that Aβ accumulation plays a major role in the cognitive decline of Tg2576 mice and support the concept of Aβ reduction therapy as a treatment of AD.—Chang, W.-P., Huang, X., Downs, D., Cirrito, J. R., Koelsch, G., Holtzman, D. M. Ghosh, A. K., Tang, J. β-Secretase inhibitor GRL-8234 rescues age-related cognitive decline in APP transgenic mice. PMID:21059748

Osteoblast Specific Overexpression of Human Interleukin-7 Rescues the Bone Mass Phenotype of Interleukin-7 Deficient Female Mice

PubMed Central

Aguila, Hector L.; Mun, Se Hwan; Kalinowski, Judith; Adams, Douglas J.; Lorenzo, Joseph A.; Lee, Sun-Kyeong

2012-01-01

Interleukin-7 is a critical cytokine for lymphoid development and a direct inhibitor of in vitro osteoclastogenesis in murine bone marrow cultures. To explore the role of IL-7 in bone, we generated transgenic mouse lines bearing the 2.3 Kb rat collagen 1A1 promoter driving the expression of human IL-7 specifically in osteoblasts. In addition we crossed these mice with IL-7 deficient mice to determine if the alterations in lymphopoiesis, bone mass and osteoclast formation observed in the IL-7 KO mice could be rescued by osteoblast-specific overexpression of IL-7. Here we show that mice overexpressing human IL-7 in the osteoblast lineage demonstrated increased trabecular bone volume in vivo by µCT and decreased osteoclast formation in vitro. Furthermore, targeted overexpression of IL-7 in osteoblasts rescued the osteopenic bone phenotype and B cell development of IL-7 KO mice but did not have an effect on T lymphopoiesis, which occurs in the periphery. The bone phenotypes in IL-7 KO mice and targeted IL-7 overexpressing mouse models were observed only in females. These results likely reflect both a direct inhibitory effects of IL-7 on osteoclastogenesis in vivo and gender specific differences in responses to IL-7. PMID:22258693

Cone-Specific Promoters for Gene Therapy of Achromatopsia and Other Retinal Diseases

PubMed Central

Ye, Guo-Jie; Budzynski, Ewa; Sonnentag, Peter; Nork, T. Michael; Sheibani, Nader; Gurel, Zafer; Boye, Sanford L.; Peterson, James J.; Boye, Shannon E.; Hauswirth, William W.; Chulay, Jeffrey D.

2016-01-01

Adeno-associated viral (AAV) vectors containing cone-specific promoters have rescued cone photoreceptor function in mouse and dog models of achromatopsia, but cone-specific promoters have not been optimized for use in primates. Using AAV vectors administered by subretinal injection, we evaluated a series of promoters based on the human L-opsin promoter, or a chimeric human cone transducin promoter, for their ability to drive gene expression of green fluorescent protein (GFP) in mice and nonhuman primates. Each of these promoters directed high-level GFP expression in mouse photoreceptors. In primates, subretinal injection of an AAV-GFP vector containing a 1.7-kb L-opsin promoter (PR1.7) achieved strong and specific GFP expression in all cone photoreceptors and was more efficient than a vector containing the 2.1-kb L-opsin promoter that was used in AAV vectors that rescued cone function in mouse and dog models of achromatopsia. A chimeric cone transducin promoter that directed strong GFP expression in mouse and dog cone photoreceptors was unable to drive GFP expression in primate cones. An AAV vector expressing a human CNGB3 gene driven by the PR1.7 promoter rescued cone function in the mouse model of achromatopsia. These results have informed the design of an AAV vector for treatment of patients with achromatopsia. PMID:26603570

Cone-Specific Promoters for Gene Therapy of Achromatopsia and Other Retinal Diseases.

PubMed

Ye, Guo-Jie; Budzynski, Ewa; Sonnentag, Peter; Nork, T Michael; Sheibani, Nader; Gurel, Zafer; Boye, Sanford L; Peterson, James J; Boye, Shannon E; Hauswirth, William W; Chulay, Jeffrey D

2016-01-01

Adeno-associated viral (AAV) vectors containing cone-specific promoters have rescued cone photoreceptor function in mouse and dog models of achromatopsia, but cone-specific promoters have not been optimized for use in primates. Using AAV vectors administered by subretinal injection, we evaluated a series of promoters based on the human L-opsin promoter, or a chimeric human cone transducin promoter, for their ability to drive gene expression of green fluorescent protein (GFP) in mice and nonhuman primates. Each of these promoters directed high-level GFP expression in mouse photoreceptors. In primates, subretinal injection of an AAV-GFP vector containing a 1.7-kb L-opsin promoter (PR1.7) achieved strong and specific GFP expression in all cone photoreceptors and was more efficient than a vector containing the 2.1-kb L-opsin promoter that was used in AAV vectors that rescued cone function in mouse and dog models of achromatopsia. A chimeric cone transducin promoter that directed strong GFP expression in mouse and dog cone photoreceptors was unable to drive GFP expression in primate cones. An AAV vector expressing a human CNGB3 gene driven by the PR1.7 promoter rescued cone function in the mouse model of achromatopsia. These results have informed the design of an AAV vector for treatment of patients with achromatopsia.

Surgical rescue: The next pillar of acute care surgery.

PubMed

Kutcher, Matthew E; Sperry, Jason L; Rosengart, Matthew R; Mohan, Deepika; Hoffman, Marcus K; Neal, Matthew D; Alarcon, Louis H; Watson, Gregory A; Puyana, Juan Carlos; Bauzá, Graciela M; Schuchert, Vaishali D; Fombona, Anisleidy; Zhou, Tianhua; Zolin, Samuel J; Becher, Robert D; Billiar, Timothy R; Forsythe, Raquel M; Zuckerbraun, Brian S; Peitzman, Andrew B

2017-02-01

The evolving field of acute care surgery (ACS) traditionally includes trauma, emergency general surgery, and critical care. However, the critical role of ACS in the rescue of patients with a surgical complication has not been explored. We here describe the role of "surgical rescue" in the practice of ACS. A prospective, electronic medical record-based ACS registry spanning January 2013 to May 2014 at a large urban academic medical center was screened by ICD-9 codes for acute surgical complications of an operative or interventional procedure. Long-term outcomes were derived from the Social Security Death Index. Of 2,410 ACS patients, 320 (13%) required "surgical rescue": most commonly, from wound complications (32%), uncontrolled sepsis (19%), and acute obstruction (15%). The majority of complications (85%) were related to an operation; 15% were related to interventional procedures. The most common rescue interventions required were bowel resection (23%), wound debridement (18%), and source control of infection (17%); 63% of patients required operative intervention, and 22% required surgical critical care. Thirty-six percent of complications occurred in ACS primary patients ("local"), whereas 38% were referred from another surgical service ("institutional") and 26% referred from another institution ("regional"). Hospital length of stay was longer, and in-hospital and 1-year mortalities were higher in rescue patients compared with those without a complication. Outcomes were equivalent between "local" and "institutional" patients, but hospital length of stay and discharge to home were significantly worse in "institutional" referrals. We here describe the distinct role of the acute care surgeon in the surgical management of complications; this is an additional pillar of ACS. In this vital role, the acute care surgeon provides crucial support to other providers as well as direct patient care in the "surgical rescue" of surgical and procedural complications. Epidemiological study, level III; therapeutic/care management study, level IV.

An unconventional origin of metal-ion rescue and inhibition in the Tetrahymena group I ribozyme reaction.

PubMed Central

Shan, S O; Herschlag, D

2000-01-01

The presence of catalytic metal ions in RNA active sites has often been inferred from metal-ion rescue of modified substrates and sometimes from inhibitory effects of alternative metal ions. Herein we report that, in the Tetrahymena group I ribozyme reaction, the deleterious effect of a thio substitution at the pro-Sp position of the reactive phosphoryl group is rescued by Mn2+. However, analysis of the reaction of this thio substrate and of substrates with other modifications strongly suggest that this rescue does not stem from a direct Mn2+ interaction with the Sp sulfur. Instead, the apparent rescue arises from a Mn2+ ion interacting with the residue immediately 3' of the cleavage site, A(+1), that stabilizes the tertiary interactions between the oligonucleotide substrate (S) and the active site. This metal site is referred to as site D herein. We also present evidence that a previously observed Ca2+ ion that inhibits the chemical step binds to metal site D. These and other observations suggest that, whereas the interactions of Mn2+ at site D are favorable for the chemical reaction, the Ca2+ at site D exerts its inhibitory effect by disrupting the alignment of the substrates within the active site. These results emphasize the vigilance necessary in the design and interpretation of metal-ion rescue and inhibition experiments. Conversely, in-depth mechanistic analysis of the effects of site-specific substrate modifications can allow the effects of specific metal ion-RNA interactions to be revealed and the properties of individual metal-ion sites to be probed, even within the sea of metal ions bound to RNA. PMID:10864040

Sequence adaptations during growth of rescued classical swine fever viruses in cell culture and within infected pigs.

PubMed

Hadsbjerg, Johanne; Friis, Martin B; Fahnøe, Ulrik; Nielsen, Jens; Belsham, Graham J; Rasmussen, Thomas Bruun

2016-08-30

Classical swine fever virus (CSFV) causes an economically important disease of swine. Four different viruses were rescued from full-length cloned cDNAs derived from the Paderborn strain of CSFV. Three of these viruses had been modified by mutagenesis (with 7 or 8 nt changes) within stem 2 of the subdomain IIIf of the internal ribosome entry site (IRES) that directs the initiation of protein synthesis. Rescued viruses were inoculated into pigs. The rescued vPader10 virus, without modifications in the IRES, induced clinical disease in pigs that was very similar to that observed previously with the parental field strain and transmission to in-contact pigs occurred. Two sequence reversions, in the NS2 and NS5B coding regions, became dominant within the virus populations in these infected pigs. Rescued viruses, with mutant IRES elements, did not induce disease and only very limited circulation of viral RNA could be detected. However, the animals inoculated with these mutant viruses seroconverted against CSFV. Thus, these mutant viruses were highly attenuated in vivo. All 4 rescued viruses were also passaged up to 20 times in cell culture. Using full genome sequencing, the same two adaptations within each of four independent virus populations were observed that restored the coding sequence to that of the parental field strain. These adaptations occurred with different kinetics. The combination of reverse genetics and in depth, full genome sequencing provides a powerful approach to analyse virus adaptation and to identify key determinants of viral replication efficiency in cells and within host animals. Copyright © 2016 Elsevier B.V. All rights reserved.

Hopping Robot with Wheels

NASA Technical Reports Server (NTRS)

Barlow, Edward; Marzwell, Nevellie; Fuller, Sawyer; Fionni, Paolo; Tretton, Andy; Burdick, Joel; Schell, Steve

2003-01-01

A small prototype mobile robot is capable of (1) hopping to move rapidly or avoid obstacles and then (2) moving relatively slowly and precisely on the ground by use of wheels in the manner of previously reported exploratory robots of the "rover" type. This robot is a descendant of a more primitive hopping robot described in "Minimally Actuated Hopping Robot" (NPO- 20911), NASA Tech Briefs, Vol. 26, No. 11 (November 2002), page 50. There are many potential applications for robots with hopping and wheeled-locomotion (roving) capabilities in diverse fields of endeavor, including agriculture, search-and-rescue operations, general military operations, removal or safe detonation of land mines, inspection, law enforcement, and scientific exploration on Earth and remote planets. The combination of hopping and roving enables this robot to move rapidly over very rugged terrain, to overcome obstacles several times its height, and then to position itself precisely next to a desired target. Before a long hop, the robot aims itself in the desired hopping azimuth and at a desired takeoff angle above horizontal. The robot approaches the target through a series of hops and short driving operations utilizing the steering wheels for precise positioning.

[Fatal outcome of an hydrogen sulfide poisoning].

PubMed

Querellou, E; Jaffrelot, M; Savary, D; Savry, C; Perfus, J-P

2005-10-01

We report a case of fatal outcome poisoning by massive exposure to hydrogen sulfide of a sewer worker. This rare event was associated with a moderate intoxication of two members of the rescue team. The death was due to asystole and massive lung oedema. Autopsy analysis showed diffuse necrotic lesions in lungs. Hydrogen sulfide is a direct and systemic poison, produced by organic matter decomposition. The direct toxicity mechanism is still unclear. The systemic toxicity is due to an acute toxicity by oxygen depletion at cellular level. It is highly diffusable and potentially very dangerous. At low concentration, rotten egg smell must trigger hydrogen sulfide suspicion since at higher concentration it is undetectable, making intoxication possible. In case of acute intoxication, there is an almost instantaneous cardiovascular failure and a rapid death. Hydrogen sulfide exposure requires prevention measures and more specifically the use of respiratory equipment for members of the rescue team.

[Implementation of telemedicine services in the earthquake disaster relief: the best medical experts provide direct medical service to the affected people].

PubMed

Li, Tan-shi; Chai, Jia-ke

2013-05-01

To sum up the experience and significance of the remote medical consultation system used by the PLA General Hospital in 4/20 Sichuan Lushan earthquake medical rescue in 2013. After the Lushan earthquake in April 20, 2013, the expert medical rescue team of the PLA General Hospital immediately took the wireless portable telemedicine system to the converge hospital which had received many wounds in earthquake and had been connected with other hospitals, medical rescue teams and rescue ambulances to open the remote medical consultation system for disaster services including intensive care, emergency treatment, orthopedics, cerebral surgery, hepatobiliary surgery, obstetrics, gynecology and other related professional remote assistance services. The experts put forward the diagnosis and treatment for victims and had a benign interaction between the experts in disaster site and rear experts, as a result improved the ability of treatment of the disaster expert medical team. The PLA General Hospital treated more than 110 patients by remote medical consultation system in the Lushan earthquake and achieved real-time HD consultation and on-site operation guide. The using of remote medical consultation system achieved the connection between multimedia communication system and medical information system of the hospital and the interconnection of video, audio, data and medical services among each united hospitals, which can provide the significant experience of using remote medical consultation system in our disaster medical rescue activities.

Partial rescue of defects in Cited2-deficient embryos by HIF-1alpha heterozygosity.

PubMed

Xu, Bing; Doughman, Yongqiu; Turakhia, Mona; Jiang, Weihong; Landsettle, Chad E; Agani, Faton H; Semenza, Gregg L; Watanabe, Michiko; Yang, Yu-Chung

2007-01-01

Hypoxia inducible factor-1 (HIF-1) initiates key cellular and tissue responses to physiological and pathological hypoxia. Evidence from in vitro and structural analyses supports a critical role for Cited2 in down-regulating HIF-1-mediated transcription by competing for binding with oxygen-sensitive HIF-1alpha to transcriptional co-activators CBP/p300. We previously detected elevated expression of HIF-1 target genes in Cited2(-/-) embryonic hearts, indicating that Cited2 inhibits HIF-1 transactivation in vivo. In this study, we show for the first time that highly hypoxic cardiac regions in mouse embryos corresponded to the sites of defects in Cited2(-/-) embryos and that defects of the outflow tract, interventricular septum, cardiac vasculature, and hyposplenia were largely rescued by HIF-1alpha haploinsufficiency. The hypoxia of the outflow tract and interventricular septum peaked at E13.5 and dissipated by E15.5 in wild-type hearts, but persisted in E15.5 Cited2(-/-) hearts. The persistent hypoxia and abnormal vasculature in the myocardium of interventricular septum in E15.5 Cited2(-/-) hearts were rescued with decreased HIF-1alpha gene dosage. Accordingly, mRNA levels of HIF-1-responsive genes were reduced in Cited2(-/-) embryonic hearts by HIF-1alpha heterozygosity. These findings suggest that a precise level of HIF-1 transcriptional activity critical for normal development is triggered by differential hypoxia and regulated through feedback inhibition by Cited2.

KSC-07pd3082

NASA Image and Video Library

2007-11-02

KENNEDY SPACE CENTER, FLA. -- During a rehearsal for the World Space Expo Aerial Salute at NASA's Kennedy Space Center, Center Director Bill Parsons (second from left) greets members of the U.S. Army Golden Knights who will demonstrate precision skydiving. Commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond, the expo will showcase various panels, presentations and educational programs, as well as the air show. Participating in the air show are the U.S. Air Force Thunderbirds, U.S. Air Force F-22 Raptor, U.S. Navy F-18 Super Hornet, U.S. Air Force F-15 Eagle, the P-51 Mustang Heritage Flight, and the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery. The U.S. Army Golden Knights will also demonstrate precision skydiving. Photo credit: NASA/George Shelton.

KSC-08pd0915

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane fly over the Launch Complex 39 area in their U.S. Navy F-18 Hornet as they return to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd0919

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane board their U.S. Navy F-18 Hornet as they prepare to return to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0922

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane prepare for takeoff of their U.S. Navy F-18 Hornet from the Shuttle Landing Facility for the return flight to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0912

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane arrive at NASA's Kennedy Space Center. Behind them is one of the U.S. Navy F-18 Hornets flown by the Blue Angels. The pilots flew into Kennedy to begin preparations for their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0916

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane arrive at NASA's Kennedy Space Center. Behind them is one of the U.S. Navy F-18 Hornets flown by the Blue Angels. The pilots flew into Kennedy to begin preparations for their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0913

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane take a last look at the center from their U.S. Navy F-18 Hornet as they return to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd0917

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane pose for the media in front of one of the U.S. Navy F-18 Hornets flown by the Blue Angels. The pilots flew into NASA's Kennedy Space Center to begin preparations for their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0911

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- A U.S. Navy F-18 Hornet taxis on the Shuttle Landing Facility runway at NASA's Kennedy Space Center. Aboard are Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane. They flew into Kennedy to begin preparations for their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0921

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane prepare for takeoff of their U.S. Navy F-18 Hornet from the Shuttle Landing Facility for the return flight to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0914

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane take a last look at the center from their U.S. Navy F-18 Hornet as they return to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Dimitri Gerondidakis

KSC-08pd0920

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane board their U.S. Navy F-18 Hornet as they prepare to return to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0923

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane take off from the Shuttle Landing Facility aboard their U.S. Navy F-18 Hornet for the return flight to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0924

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- After meeting with NASA officials about their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9, Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane take off from the Shuttle Landing Facility aboard their U.S. Navy F-18 Hornet for the return flight to their home base in Pensacola, Fla. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0918

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane arrive at NASA's Kennedy Space Center. Behind them is one of the U.S. Navy F-18 Hornets flown by the Blue Angels. The pilots flew into Kennedy to begin preparations for their involvement in the second annual Space & Air Show at Kennedy Nov. 8-9. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

KSC-08pd0910

NASA Image and Video Library

2008-04-07

CAPE CANAVERAL, Fla. -- A U.S. Navy F-18 Hornet lands at the Shuttle Landing Facility at NASA's Kennedy Space Center. Aboard are Blue Angel pilots Lt. Frank Weisser and Lt. Dan McShane. They flew into Kennedy to begin preparations for their involvement in the second annual Space & Air Show at Kennedy Space Center Nov. 8-9. The air show will be only the second time the Blue Angels have performed at Kennedy. Their precision flight team will perform high-speed passes, fast rolls, mirror formations, tight turns and their signature Delta formation showcasing the capabilities of the powerful aircraft. The 2008 Space & Air Show will include aircraft displays and space-related exhibits on the ground and plenty of action in the skies over Kennedy. Returning to the show is the 920th Rescue Wing, an Air Force Reserve Command combat search and rescue unit based at Patrick Air Force Base in Central Florida. Also, more than 20 astronauts and special guests will be on hand to personally meet guests, pose for photos and sign memorabilia. Photo credit: NASA/Kim Shiflett

Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins.

PubMed

Vitriol, Eric A; Uetrecht, Andrea C; Shen, Feimo; Jacobson, Ken; Bear, James E

2007-04-17

Chromophore-assisted laser inactivation (CALI) is a light-mediated technique that offers precise spatiotemporal control of protein inactivation, enabling better understanding of the protein's role in cell function. EGFP has been used effectively as a CALI chromophore, and its cotranslational attachment to the target protein avoids having to use exogenously added labeling reagents. A potential drawback to EGFP-CALI is that the CALI phenotype can be obscured by the endogenous, unlabeled protein that is not susceptible to light inactivation. Performing EGFP-CALI experiments in deficient cells rescued with functional EGFP-fusion proteins permits more complete loss of function to be achieved. Here, we present a modified lentiviral system for rapid and efficient generation of knockdown cell lines complemented with physiological levels of EGFP-fusion proteins. We demonstrate that CALI of EGFP-CapZbeta increases uncapped actin filaments, resulting in enhanced filament growth and the formation of numerous protrusive structures. We show that these effects are completely dependent upon knocking down the endogenous protein. We also demonstrate that CALI of EGFP-Mena in Mena/VASP-deficient cells stabilizes lamellipodial protrusions.

Dividends from Technology Applied.

ERIC Educational Resources Information Center

Aviation/Space, 1982

1982-01-01

National Aeronautics and Space Administration's (NASA) Applications Program employs aerospace science/technology to provide direct public benefit. Topics related to this program discussed include: Landsat, earth crustal study (plate tectonics), search and rescue systems, radiation measurement, upper atmosphere research, space materials processing,…

Two molecular features contribute to the Argonaute specificity for the microRNA and RNAi pathways in C. elegans.

PubMed

Jannot, Guillaume; Boisvert, Marie-Eve L; Banville, Isabelle H; Simard, Martin J

2008-05-01

In Caenorhabditis elegans, specific Argonaute proteins are dedicated to the RNAi and microRNA pathways. To uncover how the precise Argonaute selection occurs, we designed dsRNA triggers containing both miRNA and siRNA sequences. While dsRNA carrying nucleotides mismatches can only enter the miRNA pathway, a fully complementary dsRNA successfully rescues let-7 miRNA function and initiates silencing by RNAi. We demonstrated that RDE-1 is essential for RNAi induced by the perfectly paired trigger, yet is not required for silencing by the let-7 miRNA. In contrast, ALG-1/ALG-2 are required for the miRNA function, but not for the siRNA-directed gene silencing. Finally, a dsRNA containing a bulged miRNA and a perfectly paired siRNA can enter both pathways suggesting that the sorting of small RNAs occurs after that the dsRNA trigger has been processed by Dicer. Thus, our data suggest that the selection of Argonaute proteins is affected by two molecular features: (1) the structure of the small RNA duplex; and (2) the Argonautes specific characteristics.

Cyclic adenosine monophosphate metabolism in synaptic growth, strength, and precision: neural and behavioral phenotype-specific counterbalancing effects between dnc phosphodiesterase and rut adenylyl cyclase mutations.

PubMed

Ueda, Atsushi; Wu, Chun-Fang

2012-03-01

Two classic learning mutants in Drosophila, rutabaga (rut) and dunce (dnc), are defective in cyclic adenosine monophosphate (cAMP) synthesis and degradation, respectively, exhibiting a variety of neuronal and behavioral defects. We ask how the opposing effects of these mutations on cAMP levels modify subsets of phenotypes, and whether any specific phenotypes could be ameliorated by biochemical counter balancing effects in dnc rut double mutants. Our study at larval neuromuscular junctions (NMJs) demonstrates that dnc mutations caused severe defects in nerve terminal morphology, characterized by unusually large synaptic boutons and aberrant innervation patterns. Interestingly, a counterbalancing effect led to rescue of the aberrant innervation patterns but the enlarged boutons in dnc rut double mutant remained as extreme as those in dnc. In contrast to dnc, rut mutations strongly affect synaptic transmission. Focal loose-patch recording data accumulated over 4 years suggest that synaptic currents in rut boutons were characterized by unusually large temporal dispersion and a seasonal variation in the amount of transmitter release, with diminished synaptic currents in summer months. Experiments with different rearing temperatures revealed that high temperature (29-30°C) decreased synaptic transmission in rut, but did not alter dnc and wild-type (WT). Importantly, the large temporal dispersion and abnormal temperature dependence of synaptic transmission, characteristic of rut, still persisted in dnc rut double mutants. To interpret these results in a proper perspective, we reviewed previously documented differential effects of dnc and rut mutations and their genetic interactions in double mutants on a variety of physiological and behavioral phenotypes. The cases of rescue in double mutants are associated with gradual developmental and maintenance processes whereas many behavioral and physiological manifestations on faster time scales could not be rescued. We discuss factors that could contribute to the effectiveness of counterbalancing interactions between dnc and rut mutations for phenotypic rescue.

Cyclic-AMP metabolism in synaptic growth, strength and precision: Neural and behavioral phenotype-specific counterbalancing effects between dnc PDE and rut AC mutations

PubMed Central

Ueda, Atsushi; Wu, Chun-Fang

2012-01-01

Two classic learning mutants in Drosophila, rutabaga (rut) and dunce (dnc), are defective in cAMP synthesis and degradation, respectively, exhibiting a variety of neuronal and behavioral defects. We ask how the opposing effects of these mutations on cAMP levels modify subsets of phenotypes, and whether any specific phenotypes could be ameliorated by biochemical counter balancing effects in dnc rut double mutants. Our study at larval neuromuscular junctions (NMJs) demonstrate that dnc mutations caused severe defects in nerve terminal morphology, characterized by unusually large synaptic boutons and aberrant innervation patterns. Interestingly, a counterbalancing effect led to rescue of the aberrant innervation patterns but the enlarged boutons in dnc rut double mutant remained as extreme as those in dnc. In contrast to dnc, rut mutations strongly affect synaptic transmission. Focal loose-patch recording data accumulated over 4 years suggest that synaptic currents in rut boutons were characterized by unusually large temporal dispersion and a seasonal variation in the amount of transmitter release, with diminished synaptic currents in summer months. Experiments with different rearing temperatures revealed that high temperature (29–30 °C) decreased synaptic transmission in rut, but did not alter dnc and WT. Importantly, the large temporal dispersion and abnormal temperature dependence of synaptic transmission, characteristic of rut, still persisted in dnc rut double mutants. To interpret these results in a proper perspective, we reviewed previously documented differential effects of dnc and rut mutations and their genetic interactions in double mutants on a variety of physiological and behavioral phenotypes. The cases of rescue in double mutants are associated with gradual developmental and maintenance processes whereas many behavioral and physiological manifestations on faster time scales could not be rescued. We discuss factors that could contribute to the effectiveness of counter balancing interactions between dnc and rut mutations for phenotypic rescue. PMID:22380612

Intermittent fasting uncovers and rescues cognitive phenotypes in PTEN neuronal haploinsufficient mice.

PubMed

Cabral-Costa, J V; Andreotti, D Z; Mello, N P; Scavone, C; Camandola, S; Kawamoto, E M

2018-06-05

Phosphatase and tensin homolog (PTEN) is an important protein with key modulatory functions in cell growth and survival. PTEN is crucial during embryogenesis and plays a key role in the central nervous system (CNS), where it directly modulates neuronal development and synaptic plasticity. Loss of PTEN signaling function is associated with cognitive deficits and synaptic plasticity impairment. Accordingly, Pten mutations have a strong link with autism spectrum disorder. In this study, neuronal Pten haploinsufficient male mice were subjected to a long-term environmental intervention - intermittent fasting (IF) - and then evaluated for alterations in exploratory, anxiety and learning and memory behaviors. Although no significant effects on spatial memory were observed, mutant mice showed impaired contextual fear memory in the passive avoidance test - an outcome that was effectively rescued by IF. In this study, we demonstrated that IF modulation, in addition to its rescue of the memory deficit, was also required to uncover behavioral phenotypes otherwise hidden in this neuronal Pten haploinsufficiency model.

The Association Between Dissatisfaction with Debriefing and Post-Traumatic Stress Disorder (PTSD) in Rescue and Recovery Workers for the Oklahoma City Bombing.

PubMed

Tran, Duy Vu; North, Carol S

2018-03-15

The purpose of this study was to investigate potential association between psychopathology and subjective evaluation of the experience of debriefing in disaster-exposed rescue and recovery workers. Structured diagnostic interviews for DSM-III-R psychiatric disorders were conducted with 166 firefighters who served as rescue and recovery workers for the 1995 Oklahoma City bombing, who categorized their satisfaction with the debriefing on 4 levels. "Very dissatisfied" responses were examined for their association with post-traumatic stress disorder (PTSD) and with PTSD symptom groups. Being "very dissatisfied" with the debriefing was significantly associated with the DSM-III-R avoidance and numbing group and with PTSD. These findings suggest that debriefing may be an unsatisfactory intervention for people with prominent avoidance and numbing symptoms, such as those with PTSD. These individuals might be better served by referral directly to psychiatric treatment (Disaster Med Public Health Preparedness. 2018;page 1 of 5).

Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon

PubMed Central

McCann, Conor J.; Cooper, Julie E.; Natarajan, Dipa; Jevans, Benjamin; Burnett, Laura E.; Burns, Alan J.; Thapar, Nikhil

2017-01-01

Enteric nervous system neuropathy causes a wide range of severe gut motility disorders. Cell replacement of lost neurons using enteric neural stem cells (ENSC) is a possible therapy for these life-limiting disorders. Here we show rescue of gut motility after ENSC transplantation in a mouse model of human enteric neuropathy, the neuronal nitric oxide synthase (nNOS−/−) deficient mouse model, which displays slow transit in the colon. We further show that transplantation of ENSC into the colon rescues impaired colonic motility with formation of extensive networks of transplanted cells, including the development of nNOS+ neurons and subsequent restoration of nitrergic responses. Moreover, post-transplantation non-cell-autonomous mechanisms restore the numbers of interstitial cells of Cajal that are reduced in the nNOS−/− colon. These results provide the first direct evidence that ENSC transplantation can modulate the enteric neuromuscular syncytium to restore function, at the organ level, in a dysmotile gastrointestinal disease model. PMID:28671186

Reduction of Systemic Risk by Means of Pigouvian Taxation

PubMed Central

Zlatić, Vinko; Gabbi, Giampaolo; Abraham, Hrvoje

2015-01-01

We analyze the possibility of reduction of systemic risk in financial markets through Pigouvian taxation of financial institutions, which is used to support the rescue fund. We introduce the concept of the cascade risk with a clear operational definition as a subclass and a network related measure of the systemic risk. Using financial networks constructed from real Italian money market data and using realistic parameters, we show that the cascade risk can be substantially reduced by a small rate of taxation and by means of a simple strategy of the money transfer from the rescue fund to interbanking market subjects. Furthermore, we show that while negative effects on the return on investment (ROI) are direct and certain, an overall positive effect on risk adjusted return on investments (ROI RA) is visible. Please note that the taxation is introduced as a monetary/regulatory, not as a _scal measure, as the term could suggest. The rescue fund is implemented in a form of a common reserve fund. PMID:26177351

Reduction of Systemic Risk by Means of Pigouvian Taxation.

PubMed

Zlatić, Vinko; Gabbi, Giampaolo; Abraham, Hrvoje

2015-01-01

We analyze the possibility of reduction of systemic risk in financial markets through Pigouvian taxation of financial institutions, which is used to support the rescue fund. We introduce the concept of the cascade risk with a clear operational definition as a subclass and a network related measure of the systemic risk. Using financial networks constructed from real Italian money market data and using realistic parameters, we show that the cascade risk can be substantially reduced by a small rate of taxation and by means of a simple strategy of the money transfer from the rescue fund to interbanking market subjects. Furthermore, we show that while negative effects on the return on investment (ROI) are direct and certain, an overall positive effect on risk adjusted return on investments (ROIRA) is visible. Please note that the taxation is introduced as a monetary/regulatory, not as a _scal measure, as the term could suggest. The rescue fund is implemented in a form of a common reserve fund.

[Sanitary and chemical protection during the Great Patriotic War].

PubMed

Imangulov, R G; Grebeniuk, A N; Rybalko, V M; Nosov, A V

2011-05-01

During the Great Patriotic War (1941-1945) there was a real danger of use by German armies of the chemical weapon against staff of Red Army. However German command didn't risked to go on conducting large-scale chemical war against the USSR that rescued from painful death millions person. A principal cause of this decision was well organized and technically provided system of antigas protection in Red Army, including precisely organized actions of sanitary-chemical protection, qualitative preparation of military doctors on these questions and presence at them effective antidotes and other means of treatment of injuries by fighting poison gases.

The Gujarat Earthquake: Mitigations Failures and Lessons learnt for Future Strategies

NASA Astrophysics Data System (ADS)

Katuri, A. K.; Mittal, J.; Kumar, K.

Time and again, the Indian subcontinent has been suffering from diverse natural calamities, ranging from droughts to floods, landslides to earthquakes, and cyclones to spells of famines. Recently, in October 1999, a severe cyclone battered the eastern coast of Orissa affecting millions of people, blowing away homes, damaging buildings, destroying crops and wiping out a huge cattle population. The Gujarat earthquake of January 2001 was another monumental disaster that affected more than 15 million people causing colossal loss of life and property estimated at US 1.30 billion, though actual may be much higher. More than 200 international and domestic voluntary agencies promptly rushed aid to the damaged areas at the shake of the quake-2001. In this crucial rescue phase, teams were scattered across affected villages and urban centers, clueless of precise locations and extent of damage. Problems faced during the relief and rehabilitation were- absence of a comprehensive information system (both spatial and attribute), absence of a nodal agency to disseminate information on the type of relief required, absence of high precision remotely sensed data, appropriate for preparation and implementation of long term reconstruction and rehabilitation plan (Development Plan). Repeated disaster assessments by multiple agencies led to wastage of time and resources. All this led to non-coherence amongst the coordinating agencies, and rescue &relief teams. Spatial and attribute damage assessment could have been easier in the presence of comprehensive geographic and demographic information supported by high precision satellite imageries to compare pre and post disaster situation. Disaster management includes pre-disaster preparedness planning, post- disaster damage assessment, search and rescue, rehabilitation and reconstruction activities. Unlike other disasters, scientific alerts, forecasts and warnings of impending earthquake still require more attention. Disaster Preparedness Plan for speedy rescue and relief operations needs to be in place with improved information system for post disaster recovery. This paper draws upon the shortfalls faced in the management of Gujarat earthquake; a lesson learnt and presents a comprehensive strategy for Systems networking including the role of space programs in disaster management. The proposed structure is a top down approach for cooperation, emerging from bottom level demand. The missing key elements in the post-disaster situation were - effective information system, high resolution remote sensing data (for effective town planning), operational GIS, with support network from some or all of the governmental agencies. An integrated global communication network for wider dissemination of forecasts, warning and monitoring on a global level and sharing of related knowledge and information can play a vital role in disaster reduction. Needless to say, the local, regional and national disaster communication networks must be fully integrated in the global grid. The proposed structure for disaster management has a National Disaster Mitigation Establishment (NDME) as the apex body under the auspices of the central government, which would be networked across nations to similar other NDMEs. Each NDME would handle the coordination and monitoring of its state units which may be called as State Disaster Management Establishments (SDME). The SDMEs with various district or sub-district level units would collate data. The Network would be supported with field staff at its offices and would liaison with respective higher level DMEs where the lowest unit may be a village / town or cluster of villages. This paper emphasizes the need for comprehensive information system with Spatial Decision Support System (DSS) at three different levels for total disaster management.

NOAA - Search and Rescue Satellite Aided Tracking - Welcome

Science.gov Websites

through May 18, 2018 Number of People Rescued in Calendar Year 2018 in the United States: 98 Rescues at sea: 58 people rescued in 19 incidents Aviation rescues: 9 people rescued in 5 incidents Terrestrial PLB rescues: 31 people rescued in 16 incidents Worldwide - Over 43,000+ people rescued (since 1982

Fatty Acid Synthase Cooperates with Glyoxalase 1 to Protect against Sugar Toxicity

PubMed Central

Garrido, Damien; Rubin, Thomas; Poidevin, Mickael; Maroni, Brigitte; Le Rouzic, Arnaud; Parvy, Jean-Philippe; Montagne, Jacques

2015-01-01

Fatty acid (FA) metabolism is deregulated in several human diseases including metabolic syndrome, type 2 diabetes and cancers. Therefore, FA-metabolic enzymes are potential targets for drug therapy, although the consequence of these treatments must be precisely evaluated at the organismal and cellular levels. In healthy organism, synthesis of triacylglycerols (TAGs)—composed of three FA units esterified to a glycerol backbone—is increased in response to dietary sugar. Saturation in the storage and synthesis capacity of TAGs is associated with type 2 diabetes progression. Sugar toxicity likely depends on advanced-glycation-end-products (AGEs) that form through covalent bounding between amine groups and carbonyl groups of sugar or their derivatives α-oxoaldehydes. Methylglyoxal (MG) is a highly reactive α-oxoaldehyde that is derived from glycolysis through a non-enzymatic reaction. Glyoxalase 1 (Glo1) works to neutralize MG, reducing its deleterious effects. Here, we have used the power of Drosophila genetics to generate Fatty acid synthase (FASN) mutants, allowing us to investigate the consequence of this deficiency upon sugar-supplemented diets. We found that FASN mutants are lethal but can be rescued by an appropriate lipid diet. Rescued animals do not exhibit insulin resistance, are dramatically sensitive to dietary sugar and accumulate AGEs. We show that FASN and Glo1 cooperate at systemic and cell-autonomous levels to protect against sugar toxicity. We observed that the size of FASN mutant cells decreases as dietary sucrose increases. Genetic interactions at the cell-autonomous level, where glycolytic enzymes or Glo1 were manipulated in FASN mutant cells, revealed that this sugar-dependent size reduction is a direct consequence of MG-derived-AGE accumulation. In summary, our findings indicate that FASN is dispensable for cell growth if extracellular lipids are available. In contrast, FA-synthesis appears to be required to limit a cell-autonomous accumulation of MG-derived-AGEs, supporting the notion that MG is the most deleterious α-oxoaldehyde at the intracellular level. PMID:25692475

Finding a Counseling Niche.

ERIC Educational Resources Information Center

Journal of Counseling and Development, 1988

1988-01-01

Contains five personal accounts: "Answering the Cry of Disadvantaged Students" (Cheryl Bellamy); "A Change of Direction: From Researcher to Counselor in Nigeria" (Delores Mack); "Suicidal Encounters--Suicidal Experience" (Ralph Rickgarn); "Rescuing the Rescuers: First Responders at Risk" (Hal Snyder); and "Birth is Not Necessarily Painful: A…

Sandia National Laboratories: Sandia inks pact with Fire and Rescue

Science.gov Websites

; Technology Defense Systems & Assessments About Defense Systems & Assessments Program Areas Robotics R&D 100 Awards Laboratory Directed Research & Development Technology Deployment Centers Audit Sandia's Economic Impact Licensing & Technology Transfer Browse Technology Portfolios

The CDM Superfamily Protein MBC Directs Myoblast Fusion through a Mechanism That Requires Phosphatidylinositol 3,4,5-Triphosphate Binding but Is Independent of Direct Interaction with DCrk▿§

PubMed Central

Balagopalan, Lakshmi; Chen, Mei-Hui; Geisbrecht, Erika R.; Abmayr, Susan M.

2006-01-01

myoblast city (mbc), a member of the CDM superfamily, is essential in the Drosophila melanogaster embryo for fusion of myoblasts into multinucleate fibers. Using germ line clones in which both maternal and zygotic contributions were eliminated and rescue of the zygotic loss-of-function phenotype, we established that mbc is required in the fusion-competent subset of myoblasts. Along with its close orthologs Dock180 and CED-5, MBC has an SH3 domain at its N terminus, conserved internal domains termed DHR1 and DHR2 (or “Docker”), and C-terminal proline-rich domains that associate with the adapter protein DCrk. The importance of these domains has been evaluated by the ability of MBC mutations and deletions to rescue the mbc loss-of-function muscle phenotype. We demonstrate that the SH3 and Docker domains are essential. Moreover, ethyl methanesulfonate-induced mutations that change amino acids within the MBC Docker domain to residues that are conserved in other CDM family members nevertheless eliminate MBC function in the embryo, which suggests that these sites may mediate interactions specific to Drosophila MBC. A functional requirement for the conserved DHR1 domain, which binds to phosphatidylinositol 3,4,5-triphosphate, implicates phosphoinositide signaling in myoblast fusion. Finally, the proline-rich C-terminal sites mediate strong interactions with DCrk, as expected. These sites are not required for MBC to rescue the muscle loss-of-function phenotype, however, which suggests that MBC's role in myoblast fusion can be carried out independently of direct DCrk binding. PMID:17030600

A Precision Medicine Approach to the Rescue of Function on Malignant Calmodulinopathic Long QT Syndrome

PubMed Central

Limpitikul, Worawan B.; Dick, Ivy E.; Tester, David J.; Boczek, Nicole J.; Limphong, Pattraranee; Yang, Wanjun; Choi, Myoung Hyun; Babich, Jennifer; DiSilvestre, Deborah; Kanter, Ronald J.; Tomaselli, Gordon F.; Ackerman, Michael J.; Yue, David T.

2017-01-01

Rationale Calmodulinopathies comprise a new category of potentially life-threatening genetic arrhythmia syndromes capable of producing severe long QT syndrome (LQTS) with mutations involving either CALM1, CALM2, or CALM3. The underlying basis of this form of LQTS is a disruption of Ca2+/CaM-dependent inactivation (CDI) of L-type Ca2+ channels (LTCCs). Objective To gain insight into the mechanistic underpinnings of calmodulinopathies and devise new therapeutic strategies for the treatment of this form of LQTS. Methods and Results We generated and characterized the functional properties of iPSC-derived cardiomyocytes (iPSC-CMs) from a patient with D130G-CALM2-mediated LQTS, thus creating a platform with which to devise and test novel therapeutic strategies. The patient-derived iPSC-CMs display (1) significantly prolonged action potentials (APs), (2) disrupted Ca2+ cycling properties, and (3) diminished CDI of LTCCs. Next, taking advantage of the fact that calmodulinopathy patients harbor a mutation in only one of six redundant CaM-encoding alleles, we devised a strategy using CRISPR interference (CRISPRi) to selectively suppress the mutant gene while sparing the wild-type counterparts. Indeed, suppression of CALM2 expression produced a functional rescue in iPSC-CMs with D130G-CALM2, as shown by the normalization of AP duration and CDI following treatment. Moreover, CRISPRi can be designed to achieve selective knockdown of any of the three CALM genes, making it a generalizable therapeutic strategy for any calmodulinopathy. Conclusions Overall, this therapeutic strategy holds great promise for calmodulinopathy patients as it represents a generalizable intervention capable of specifically altering CaM expression and potentially attenuating LQTS-triggered cardiac events, thus initiating a path towards precision medicine. PMID:27765793

Searching Lost People with Uavs: the System and Results of the Close-Search Project

NASA Astrophysics Data System (ADS)

Molina, P.; Colomina, I.; Vitoria, T.; Silva, P. F.; Skaloud, J.; Kornus, W.; Prades, R.; Aguilera, C.

2012-07-01

This paper will introduce the goals, concept and results of the project named CLOSE-SEARCH, which stands for 'Accurate and safe EGNOS-SoL Navigation for UAV-based low-cost Search-And-Rescue (SAR) operations'. The main goal is to integrate a medium-size, helicopter-type Unmanned Aerial Vehicle (UAV), a thermal imaging sensor and an EGNOS-based multi-sensor navigation system, including an Autonomous Integrity Monitoring (AIM) capability, to support search operations in difficult-to-access areas and/or night operations. The focus of the paper is three-fold. Firstly, the operational and technical challenges of the proposed approach are discussed, such as ultra-safe multi-sensor navigation system, the use of combined thermal and optical vision (infrared plus visible) for person recognition and Beyond-Line-Of-Sight communications among others. Secondly, the implementation of the integrity concept for UAV platforms is discussed herein through the AIM approach. Based on the potential of the geodetic quality analysis and on the use of the European EGNOS system as a navigation performance starting point, AIM approaches integrity from the precision standpoint; that is, the derivation of Horizontal and Vertical Protection Levels (HPLs, VPLs) from a realistic precision estimation of the position parameters is performed and compared to predefined Alert Limits (ALs). Finally, some results from the project test campaigns are described to report on particular project achievements. Together with actual Search-and-Rescue teams, the system was operated in realistic, user-chosen test scenarios. In this context, and specially focusing on the EGNOS-based UAV navigation, the AIM capability and also the RGB/thermal imaging subsystem, a summary of the results is presented.

Budget impact analysis of rivaroxaban vs. warfarin anticoagulation strategy for direct current cardioversion in non-valvular atrial fibrillation patients: the MonaldiVert Economic Study.

PubMed

Russo, Vincenzo; Rago, Anna; Papa, Andrea A; Bianchi, Valter; Tavoletta, Vincenzo; DE Vivo, Stefano; Cavallaro, Ciro; Nigro, Gerardo; D'Onofrio, Antonio

2018-02-01

Rivaroxaban is the first novel oral anticoagulant to receive regulatory approval for non-valvular atrial fibrillation (NVAF) patients who require cardioversion. The MonaldiVert real life experience showed positive benefit-risk profile of short term rivaroxaban administration for transesophageal echocardiogram guided cardioversion in patients who had not achieved adequate pre-procedural vitamin K antagonist (VKA) anticoagulation. Aim of our study was to perform a budget impact analysis of MonaldiVert anticoagulation strategy for direct current cardioversion in NVAF patients and to compare the following costs borne by the Regional Healthcare System (RHS) with those for a hypothetical cohort of identical patients underwent from the beginning to early rivaroxaban treatment before direct current cardioversion. The mean costs per each NVAF patient treated with VKA strategy and rivaroxaban rescue strategy were € 134.53 and € 189.83, respectively. Considering a hypothetical scenario in which all study population would be treated from the beginning with rivaroxaban (rivaroxaban early strategy), the mean cost per patient would have been € 81.11. The total cost borne by the RHS, including the cost of the cardioversion procedure, for the two therapeutic strategies carried out at Monaldi Hospital (VKA strategy and Rivaroxaban rescue strategy) was € 88,458.53. The total cost would be borne by the RHS for rivaroxaban early strategy, if applied to all study population, would have been € 69,989.15 with a saving of € 18,469.38 compared to the actually applied strategy. Rivaroxaban rescue strategy for transesophageal echocardiography guided direct current cardioversion in NVAF patients, who had not achieved adequate pre-procedural VKA anticoagulation, is an effective and safe strategy, which allows to not delay the procedure, reducing times and wastage of cardioversion slots, without substantial costs increase.

Dock and Pak regulate olfactory axon pathfinding in Drosophila.

PubMed

Ang, Lay-Hong; Kim, Jenny; Stepensky, Vitaly; Hing, Huey

2003-04-01

The convergence of olfactory axons expressing particular odorant receptor (Or) genes on spatially invariant glomeruli in the brain is one of the most dramatic examples of precise axon targeting in developmental neurobiology. The cellular and molecular mechanisms by which olfactory axons pathfind to their targets are poorly understood. We report here that the SH2/SH3 adapter Dock and the serine/threonine kinase Pak are necessary for the precise guidance of olfactory axons. Using antibody localization, mosaic analyses and cell-type specific rescue, we observed that Dock and Pak are expressed in olfactory axons and function autonomously in olfactory neurons to regulate the precise wiring of the olfactory map. Detailed analyses of the mutant phenotypes in whole mutants and in small multicellular clones indicate that Dock and Pak do not control olfactory neuron (ON) differentiation, but specifically regulate multiple aspects of axon trajectories to guide them to their cognate glomeruli. Structure/function studies show that Dock and Pak form a signaling pathway that mediates the response of olfactory axons to guidance cues in the developing antennal lobe (AL). Our findings therefore identify a central signaling module that is used by ONs to project to their cognate glomeruli.

Direction information in multiple object tracking is limited by a graded resource.

PubMed

Horowitz, Todd S; Cohen, Michael A

2010-10-01

Is multiple object tracking (MOT) limited by a fixed set of structures (slots), a limited but divisible resource, or both? Here, we answer this question by measuring the precision of the direction representation for tracked targets. The signature of a limited resource is a decrease in precision as the square root of the tracking load. The signature of fixed slots is a fixed precision. Hybrid models predict a rapid decrease to asymptotic precision. In two experiments, observers tracked moving disks and reported target motion direction by adjusting a probe arrow. We derived the precision of representation of correctly tracked targets using a mixture distribution analysis. Precision declined with target load according to the square-root law up to six targets. This finding is inconsistent with both pure and hybrid slot models. Instead, directional information in MOT appears to be limited by a continuously divisible resource.

Observational Case Series Evaluation of the Granisetron Transdermal Patch System (Sancuso) for the Management of Nausea/Vomiting of Pregnancy.

PubMed

Le, Tran N; Adler, Michael T; Ouillette, Holly; Berens, Pamela; Smith, Judith A

2017-07-01

Objective  The objective of this study was to observe the efficacy of antiemetic therapy (no emesis/retching episodes and no rescue medication use) when granisetron is administered via a transdermal patch system (TDS) in women who are 6 to 14 weeks pregnant when compared with oral ondansetron by evaluating the frequency of the use of rescue medications for control of nausea/vomiting of pregnancy (NVP). Methods  This was an observational case series study to observe the potential benefits of granisetron TDS compared with oral ondansetron for management of NVP in pregnant patients during the first trimester. Dates of data collection were September 1, 2014, through December 31, 2015. There was no direct contact with patient. The oral ondansetron and granisetron TDS patients were matched by age, 4:1. The proportion of patients who received rescue antiemetics was calculated from those patients who continued to experience NVP. Risk factors for NVP were identified and compared between groups. Descriptive statistics were used to describe study results. Results  Patients were prescribed rescue antiemetics in 0/3 patients in the granisetron TDS group compared with 2/12 patients in the oral ondansetron group. Conclusion  Prospective efficacy studies on the use of granisetron TDS for management of NVP are needed to confirm this clinical observation. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Basic examination of a technique to visualize space filled with dense smoke using millimeter-wave radar

NASA Astrophysics Data System (ADS)

Omine, Yukio; Sakai, Masaki; Aoki, Yoshimitsu; Takagi, Mikio

2004-10-01

In recent years, crisis management in response to terrorist attacks and natural disasters, as well as accelerating rescue operations has become an important issue. Rescue operations greatly influence human lives, and require the ability to accurately and swiftly communicate information as well as assess the status of the site. Currently, considerable amount of research is being conducted for assisting rescue operations, with the application of various engineering techniques such as information technology and radar technology. In the present research, we believe that assessing the status of the site is most crucial in rescue and firefighting operations at a fire disaster site, and aim to visualize the space that is smothered with dense smoke. In a space filled with dense smoke, where visual or infrared sensing techniques are not feasible, three-dimensional measurements can be realized using a compact millimeter wave radar device combined with directional information from a gyro sensor. Using these techniques, we construct a system that can build and visualize a three-dimensional geometric model of the space. The final objective is to implement such a system on a wearable computer, which will improve the firefighters' spatial perception, assisting them in the baseline assessment and the decision-making process. In the present paper, we report the results of the basic experiments on three-dimensional measurement and visualization of a space that is smoke free, using a millimeter wave radar.

A chemical corrector modifies the channel function of F508del-CFTR.

PubMed

Kim Chiaw, Patrick; Wellhauser, Leigh; Huan, Ling Jun; Ramjeesingh, Mohabir; Bear, Christine E

2010-09-01

The deletion of Phe-508 (F508del) constitutes the most prevalent cystic fibrosis-causing mutation. This mutation leads to cystic fibrosis transmembrane conductance regulator (CFTR) misfolding and retention in the endoplasmic reticulum and altered channel activity in mammalian cells. This folding defect can however be partially overcome by growing cells expressing this mutant protein at low (27 degrees C) temperature. Chemical "correctors" have been identified that are also effective in rescuing the biosynthetic defect in F508del-CFTR, thereby permitting its functional expression at the cell surface. The mechanism of action of chemical correctors remains unclear, but it has been suggested that certain correctors [including 4-cyclohexyloxy-2-(1-[4-(4-methoxy-benzenesulfonyl)-piperazin-1-yl]-ethyl)-quinazoline (VRT-325)] may act to promote trafficking by interacting directly with the mutant protein. To test this hypothesis, we assessed the effect of VRT-325 addition on the channel activity of F508del-CFTR after its surface expression had been "rescued" by low temperature. It is noteworthy that short-term pretreatment with VRT-325 [but not with an inactive analog, 4-hydroxy-2-(1-[4-(4-methoxy-benzenesulfonyl)-piperazin-1-yl]-ethyl)-quinazoline (VRT-186)], caused a modest but significant inhibition of cAMP-mediated halide flux. Furthermore, VRT-325 decreased the apparent ATP affinity of purified and reconstituted F508del-CFTR in our ATPase activity assay, an effect that may account for the decrease in channel activity by temperature-rescued F508del-CFTR. These findings suggest that biosynthetic rescue mediated by VRT-325 may be conferred (at least in part) by direct modification of the structure of the mutant protein, leading to a decrease in its ATP-dependent conformational dynamics. Therefore, the challenge for therapy discovery will be the design of small molecules that bind to promote biosynthetic maturation of the major mutant without compromising its activity in vivo.

Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β.

PubMed

Wu, Xiaoyang; Shen, Qing-Tao; Oristian, Daniel S; Lu, Catherine P; Zheng, Qinsi; Wang, Hong-Wei; Fuchs, Elaine

2011-02-04

Homeostasis and wound healing rely on stem cells (SCs) whose activity and directed migration are often governed by Wnt signaling. In dissecting how this pathway integrates with the necessary downstream cytoskeletal dynamics, we discovered that GSK3β, a kinase inhibited by Wnt signaling, directly phosphorylates ACF7, a > 500 kDa microtubule-actin crosslinking protein abundant in hair follicle stem cells (HF-SCs). We map ACF7's GSK3β sites to the microtubule-binding domain and show that phosphorylation uncouples ACF7 from microtubules. Phosphorylation-refractile ACF7 rescues overall microtubule architecture, but phosphorylation-constitutive mutants do not. Neither mutant rescues polarized movement, revealing that phospho-regulation must be dynamic. This circuitry is physiologically relevant and depends upon polarized GSK3β inhibition at the migrating front of SCs/progeny streaming from HFs during wound repair. Moreover, only ACF7 and not GSKβ-refractile-ACF7 restore polarized microtubule-growth and SC-migration to ACF7 null skin. Our findings provide insights into how this conserved spectraplakin integrates signaling, cytoskeletal dynamics, and polarized locomotion of somatic SCs. Copyright © 2011 Elsevier Inc. All rights reserved.

KSC-07pd3126

NASA Image and Video Library

2007-11-04

KENNEDY SPACE CENTER, FLA. -- Near the NASA Causeway at NASA's Kennedy Space Center, part of the U.S. Air Force 920th Rescue Wing puts on a rescue demonstration during the World Space Expo aerial salute. This unit was responsible for Mercury and Gemini capsule recovery. Other aircraft joining in the expo salute include the U.S. Air Force Thunderbirds Demonstration Squadron, the U.S. Navy F-18 Super Hornets, U.S. Air Force F-22 Raptor, U.S. Air Force F-15 Eagle, P-51 Mustang Heritage Flight, and the U.S. Army Golden Knights demonstrating precision skydiving. The World Space Expo held Nov. 1-4 was an event commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond. The expo showcased various panels, presentations and educational programs. It also was a part of NASA's 50th anniversary celebrations, highlighting the 45th Anniversary of the Mercury Program celebration featuring original NASA astronauts John Glenn and Scott Carpenter and the Pioneering Women of Aerospace forum featuring Eileen Collins and other prominent female space veterans. The agency was founded Oct. 1, 1958. Photo credit: NASA/Kim Shiflett

KSC-07pd3125

NASA Image and Video Library

2007-11-04

KENNEDY SPACE CENTER, FLA. -- Crowds fill the areas along NASA Causeway at NASA's Kennedy Space Center to watch the World Space Expo aerial salute. Seen in flight is the plane used to refuel rescue helicopters in mid-air. Aircraft joining in the expo salute include the U.S. Air Force Thunderbirds Demonstration Squadron, the U.S. Navy F-18 Super Hornets, U.S. Air Force F-22 Raptor, U.S. Air Force F-15 Eagle, P-51 Mustang Heritage Flight, the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery, and the U.S. Army Golden Knights precision skydivers. The World Space Expo held Nov. 1-4 was an event commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond. The expo showcased various panels, presentations and educational programs. It also was a part of NASA's 50th anniversary celebrations, highlighting the 45th Anniversary of the Mercury Program celebration featuring original NASA astronauts John Glenn and Scott Carpenter and the Pioneering Women of Aerospace forum featuring Eileen Collins and other prominent female space veterans. The agency was founded Oct. 1, 1958. Photo credit: NASA/Kim Shiflett

Clinically relevant hypoglycemia prediction metrics for event mitigation.

PubMed

Harvey, Rebecca A; Dassau, Eyal; Zisser, Howard C; Bevier, Wendy; Seborg, Dale E; Jovanovič, Lois; Doyle, Francis J

2012-08-01

The purpose of this study was to develop a method to compare hypoglycemia prediction algorithms and choose parameter settings for different applications, such as triggering insulin pump suspension or alerting for rescue carbohydrate treatment. Hypoglycemia prediction algorithms with different parameter settings were implemented on an ambulatory dataset containing 490 days from 30 subjects with type 1 diabetes mellitus using the Dexcom™ (San Diego, CA) SEVEN™ continuous glucose monitoring system. The performance was evaluated using a proposed set of metrics representing the true-positive ratio, false-positive rate, and distribution of warning times. A prospective, in silico study was performed to show the effect of using different parameter settings to prevent or rescue from hypoglycemia. The retrospective study results suggest the parameter settings for different methods of hypoglycemia mitigation. When rescue carbohydrates are used, a high true-positive ratio, a minimal false-positive rate, and alarms with short warning time are desired. These objectives were met with a 30-min prediction horizon and two successive flags required to alarm: 78% of events were detected with 3.0 false alarms/day and 66% probability of alarms occurring within 30 min of the event. This parameter setting selection was confirmed in silico: treating with rescue carbohydrates reduced the duration of hypoglycemia from 14.9% to 0.5%. However, for a different method, such as pump suspension, this parameter setting only reduced hypoglycemia to 8.7%, as can be expected by the low probability of alarming more than 30 min ahead. The proposed metrics allow direct comparison of hypoglycemia prediction algorithms and selection of parameter settings for different types of hypoglycemia mitigation, as shown in the prospective in silico study in which hypoglycemia was alerted or treated with rescue carbohydrates.

Mitochondrial mechanisms of neuronal rescue by F-68, a hydrophilic Pluronic block co-polymer, following acute substrate deprivation.

PubMed

Wang, Janice C; Bindokas, Vytautas P; Skinner, Matthew; Emrick, Todd; Marks, Jeremy D

2017-10-01

Global brain ischemia can lead to widespread neuronal death and poor neurologic outcomes in patients. Despite detailed understanding of the cellular and molecular mechanisms mediating neuronal death following focal and global brain hypoxia-ischemia, treatments to reduce ischemia-induced brain injury remain elusive. One pathway central to neuronal death following global brain ischemia is mitochondrial dysfunction, one consequence of which is the cascade of intracellular events leading to mitochondrial outer membrane permeabilization. A novel approach to rescuing injured neurons from death involves targeting cellular membranes using a class of synthetic molecules called Pluronics. Pluronics are triblock copolymers of hydrophilic poly[ethylene oxide] (PEO) and hydrophobic poly[propylene oxide] (PPO). Evidence is accumulating to suggest that hydrophilic Pluronics rescue injured neurons from death following substrate deprivation by preventing mitochondrial dysfunction. Here, we will review current understanding of the nature of interaction of Pluronic molecules with biological membranes and the efficacy of F-68, an 80% hydrophilic Pluronic, in rescuing neurons from injury. We will review data indicating that F-68 reduces mitochondrial dysfunction and mitochondria-dependent death pathways in a model of neuronal injury in vitro, and present new evidence that F-68 acts directly on mitochondria to inhibit mitochondrial outer membrane permeabilization. Finally, we will present results of a pilot, proof-of-principle study suggesting that F-68 is effective in reducing hippocampal injury induced by transient global ischemia in vivo. By targeting mitochondrial dysfunction, F-68 and other Pluronic molecules constitute an exciting new approach to rescuing neurons from acute injury. Copyright © 2017 Elsevier Ltd. All rights reserved.

14 CFR 93.313 - Communications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Communications. 93.313 Section 93.313 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... directed by the U.S. Air Force Rescue Coordination Center, no person may operate an aircraft in the Special...

14 CFR 93.313 - Communications.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Communications. 93.313 Section 93.313 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... directed by the U.S. Air Force Rescue Coordination Center, no person may operate an aircraft in the Special...

14 CFR 93.313 - Communications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Communications. 93.313 Section 93.313 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... directed by the U.S. Air Force Rescue Coordination Center, no person may operate an aircraft in the Special...

14 CFR 93.313 - Communications.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Communications. 93.313 Section 93.313 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... directed by the U.S. Air Force Rescue Coordination Center, no person may operate an aircraft in the Special...

14 CFR 93.313 - Communications.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Communications. 93.313 Section 93.313 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... directed by the U.S. Air Force Rescue Coordination Center, no person may operate an aircraft in the Special...

Leadership lessons from the Chilean mine rescue.

PubMed

Rashid, Faaiza; Edmondson, Amy C; Leonard, Herman B

2013-01-01

Three years ago, when a cave-in at the San José mine in Chile trapped 33 men under 700,000 metric tons of rock, experts estimated the probability of getting them out alive at less than 1%. Yet, after spending a record 69 days underground, all 33 were hoisted up to safety. The inspiring story of their rescue is a case study in how to lead in situations where the stakes, risk, and uncertainty are incredibly high and time pressure is intense. Today executives often find themselves in similar straits. When they do, many feel torn. Should they be directive, taking charge and commanding action? Or should they be empowering, enabling innovation and experimentation? As the successful example of André Sougarret, the chief of the mine rescue operation, shows, the answer is yes--to both. The choice is a false dichotomy. Implementing this dual approach involves three key tasks. Each has directive and enabling components. The first task is envisioning, which requires instilling both realism and hope. The second task is enrolling, which means setting clear boundaries for who is on and off the team, but inviting in helpful collaborators. The third task is engaging--leading disciplined execution while encouraging innovation and experimentation. The authors of this article describe how Sougarret ably juggled all of these tasks, orchestrating the efforts of hundreds of people from different organizations, areas of expertise, and countries in an extraordinary mission that overcame impossible odds.

A control system for orbiting tethered-body operations

NASA Technical Reports Server (NTRS)

Eades, J. B., Jr.

1975-01-01

This paper shows that through proper control logic the transfer of men and cargo between spacecrafts, or the 'positioning of packages' adjacent to orbiters, can be accomodated safely and predictably using tethers. Also, these systems may be adapted to rescue and retrieval operations where 'controlled motions' must be maintained. Shown here is a method which illustrates how tethered-body motions are controlled for 'reel-in' and 'reel-out' operations, and for precise 'positioning' purposes. Three control modes are examined; from these are derived sets of universal control parameters capable of predescribing systems of similar types. In addition, these parameters form a basis for designing tethered-body systems and operations.

A principled approach to the stranded cost issue

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maloney, M.T.; Sauer, R.D.

1998-04-01

The case against stranded cost recovery is a strong one, whether investments in generation were efficient ex ante or not. This is the unavoidable conclusion once the proper roles of the regulator, utilities, investors, and consumers in the regulatory system of the past century are clearly identified. It is improper and inefficient for government to sanction the mistakes of the private sector by taxing consumers in order to rescue producers. This is precisely what stranded cost recovery does. Denial of stranded cost recovery is consistent with the role of the regulator as a substitute for salutary market forces and, indeed,more » is required by it.« less

Autonomous unmanned air vehicles (UAV) techniques

NASA Astrophysics Data System (ADS)

Hsu, Ming-Kai; Lee, Ting N.

2007-04-01

The UAVs (Unmanned Air Vehicles) have great potentials in different civilian applications, such as oil pipeline surveillance, precision farming, forest fire fighting (yearly), search and rescue, boarder patrol, etc. The related industries of UAVs can create billions of dollars for each year. However, the road block of adopting UAVs is that it is against FAA (Federal Aviation Administration) and ATC (Air Traffic Control) regulations. In this paper, we have reviewed the latest technologies and researches on UAV navigation and obstacle avoidance. We have purposed a system design of Jittering Mosaic Image Processing (JMIP) with stereo vision and optical flow to fulfill the functionalities of autonomous UAVs.

46 CFR 160.156-11 - Fabrication of prototype rescue boats and fast rescue boats for approval.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Fabrication of prototype rescue boats and fast rescue boats for approval. 160.156-11 Section 160.156-11 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Boats and Fast Rescue Boats (SOLAS) § 160.156-11 Fabrication of prototype rescue boats and fast rescue...

46 CFR 160.156-13 - Approval inspections and tests for prototype rescue boats and fast rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... boats and fast rescue boats. 160.156-13 Section 160.156-13 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-13 Approval inspections and tests for prototype rescue boats and fast rescue boats. (a) After the Commandant notifies the manufacturer that the...

46 CFR 160.156-11 - Fabrication of prototype rescue boats and fast rescue boats for approval.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Fabrication of prototype rescue boats and fast rescue boats for approval. 160.156-11 Section 160.156-11 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Boats and Fast Rescue Boats (SOLAS) § 160.156-11 Fabrication of prototype rescue boats and fast rescue...

46 CFR 160.156-11 - Fabrication of prototype rescue boats and fast rescue boats for approval.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Fabrication of prototype rescue boats and fast rescue boats for approval. 160.156-11 Section 160.156-11 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Boats and Fast Rescue Boats (SOLAS) § 160.156-11 Fabrication of prototype rescue boats and fast rescue...

46 CFR 160.156-13 - Approval inspections and tests for prototype rescue boats and fast rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... boats and fast rescue boats. 160.156-13 Section 160.156-13 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-13 Approval inspections and tests for prototype rescue boats and fast rescue boats. (a) After the Commandant notifies the manufacturer that the...

46 CFR 160.156-13 - Approval inspections and tests for prototype rescue boats and fast rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... boats and fast rescue boats. 160.156-13 Section 160.156-13 Shipping COAST GUARD, DEPARTMENT OF HOMELAND... EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-13 Approval inspections and tests for prototype rescue boats and fast rescue boats. (a) After the Commandant notifies the manufacturer that the...

Infectious disease and dermatologic conditions in evacuees and rescue workers after Hurricane Katrina--multiple states, August-September, 2005.

PubMed

2005-09-30

On August 29, 2005, Hurricane Katrina struck states along the Gulf Coast of the United States. In the days after the hurricane struck, approximately 750 evacuation centers were established in at least 18 states to accommodate more than 200,000 evacuees. State and local health departments, with assistance from CDC, initiated enhanced infectious disease surveillance and outbreak response activities, implemented by teams of public health and rescue workers, including military personnel. Outbreak monitoring included direct reporting of conditions of public health significance to public health agencies; daily contact between CDC and local public health officials; canvassing of reports from CDC, public health departments, and news media for potential infectious disease outbreaks; and investigation of reports of infectious disease with outbreak potential. This report summarizes infectious disease and dermatologic conditions reported during the first 3 weeks after the hurricane, before effective local surveillance was fully implemented. One outbreak of norovirus was reported among evacuees in Texas; no other outbreaks requiring unusual mobilization of public health resources were reported among evacuees or rescue workers.

Winter color polymorphisms identify global hot spots for evolutionary rescue from climate change.

PubMed

Mills, L Scott; Bragina, Eugenia V; Kumar, Alexander V; Zimova, Marketa; Lafferty, Diana J R; Feltner, Jennifer; Davis, Brandon M; Hackländer, Klaus; Alves, Paulo C; Good, Jeffrey M; Melo-Ferreira, José; Dietz, Andreas; Abramov, Alexei V; Lopatina, Natalia; Fay, Kairsten

2018-03-02

Maintenance of biodiversity in a rapidly changing climate will depend on the efficacy of evolutionary rescue, whereby population declines due to abrupt environmental change are reversed by shifts in genetically driven adaptive traits. However, a lack of traits known to be under direct selection by anthropogenic climate change has limited the incorporation of evolutionary processes into global conservation efforts. In 21 vertebrate species, some individuals undergo a seasonal color molt from summer brown to winter white as camouflage against snow, whereas other individuals remain brown. Seasonal snow duration is decreasing globally, and fitness is lower for winter white animals on snowless backgrounds. Based on 2713 georeferenced samples of known winter coat color-from eight species across trophic levels-we identify environmentally driven clinal gradients in winter coat color, including polymorphic zones where winter brown and white morphs co-occur. These polymorphic zones, underrepresented by existing global protected area networks, indicate hot spots for evolutionary rescue in a changing climate. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Model for Bi-objective emergency rescue vehicle routing optimization

NASA Astrophysics Data System (ADS)

Yang, Yuhang

2017-03-01

Vehicle routing problem is an important research topic in management science. In this paper, one vehicle can rescue multiple disaster points and two optimization objectives are rescue time and rescue effect. Rescue effect is expressed as the ratio of unloaded material to arrival time when rescue vehicles participate in rescue every time. In this paper, the corresponding emergency rescue model is established and the effectiveness of the model is verified by simulated annealing algorithm. It can provide the basis for practical decision-making.

Failure to Rescue, Rescue Surgery and Centralization of Postoperative Complications: A Challenge for General and Acute Care Surgeons.

PubMed

Zago, Mauro; Bozzo, Samantha; Carrara, Giulia; Mariani, Diego

2017-01-01

To explore the current literature on the failure to rescue and rescue surgery concepts, to identify the key items for decreasing the failure to rescue rate and improve outcome, to verify if there is a rationale for centralization of patients suffering postoperative complications. There is a growing awareness about the need to assess and measure the failure to rescue rate, on institutional, regional and national basis. Many factors affect failure to rescue, and all should be individually analyzed and considered. Rescue surgery is one of these factors. Rescue surgery assumes an acute care surgery background. Measurement of failure to rescue rate should become a standard for quality improvement programs. Implementation of all clinical and organizational items involved is the key for better outcomes. Preparedness for rescue surgery is a main pillar in this process. Centralization of management, audit, and communication are important as much as patient centralization. Celsius.

Rescue Manual. Module 8.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. Instructional Materials Lab.

This learner manual for rescuers covers the current techniques or practices required in the rescue service. The eighth of 10 modules contains 6 chapters: (1) trench rescue; (2) shoring and tunneling techniques; (3) farm accident rescue; (4) wilderness search and rescue; (5) aircraft rescue; and (6) helicopter information. Key points, an…

Respiratory Emergencies and Management of Mining Accidents

PubMed Central

Özmen, İpek; Aksoy, Emine

2015-01-01

The rapid detection of the reasons for mining accidents that lead to emergency situations is vital for search and rescue work. The control of fire and gas leakage provides an immediate approach for rescue works for deaths or injuries and the detection of who needs resuscitation outside of the mine. The evacuation and recovery operations should be directed by continuous monitoring of the mine environment due to fire and explosion risks. The main toxic gases in mines are carbon monoxide (CO) and carbon dioxide (CO2); the flammable gases are methane (CH4), CO, and hydrogen (H2); the suffocating gases are CO2, nitrogen (N20), and CH4; and the toxic gases are CO, nitrogen oxides (NOx), and hydrogen sulfide (H2S). PMID:29404110

Coping, functioning, and adjustment of rescue workers after the Oklahoma City bombing.

PubMed

North, Carol S; Tivis, Laura; McMillen, J Curtis; Pfefferbaum, Betty; Cox, Jann; Spitznagel, Edward L; Bunch, Kenneth; Schorr, John; Smith, Elizabeth M

2002-06-01

Studies have not previously considered postdisaster adjustment in the context of psychiatric disorders. After the Oklahoma City bombing, a volunteer sample of 181 firefighters who served as rescue and recovery workers was assessed with a structured diagnostic interview. The firefighters had relatively low rates of posttraumatic stress disorder (PTSD) and described little functional impairment, positive social adjustment, and high job satisfaction. PTSD was associated with reduced job satisfaction and functional impairment, providing diagnostic validity. Turning to social supports, seeking mental health treatment, and taking medication were not widely prevalent coping responses. Postdisaster alcohol use disorders and drinking to cope were significantly associated with indicators of poorer functioning. Surveillance for problem drinking after disaster exposure may identify useful directions for intervention.

78 FR 35974 - Proposed Information Collection; Comment Request; Coal Mine Rescue Teams; Arrangements for...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-14

... Request; Coal Mine Rescue Teams; Arrangements for Emergency Medical Assistance and Transportation for... Part 49, Mine Rescue Teams, Subpart B--Mine Rescue Teams for Underground Coal Mines, sets standards related to the availability of mine rescue teams; alternate mine rescue capability for small and remote...

30 CFR 48.8 - Annual refresher training of miners; minimum courses of instruction; hours of instruction.

Code of Federal Regulations, 2014 CFR

2014-07-01

... controls and communication systems. The course shall include instruction on the procedures for riding on... the use of the mine communication systems, warning signals, and directional signs. (3) Barricading... environment. (8) Self-rescue and respiratory devices. The course shall include instruction and demonstration...

30 CFR 48.8 - Annual refresher training of miners; minimum courses of instruction; hours of instruction.

Code of Federal Regulations, 2013 CFR

2013-07-01

... controls and communication systems. The course shall include instruction on the procedures for riding on... the use of the mine communication systems, warning signals, and directional signs. (3) Barricading... environment. (8) Self-rescue and respiratory devices. The course shall include instruction and demonstration...

30 CFR 48.8 - Annual refresher training of miners; minimum courses of instruction; hours of instruction.

Code of Federal Regulations, 2011 CFR

2011-07-01

... controls and communication systems. The course shall include instruction on the procedures for riding on... the use of the mine communication systems, warning signals, and directional signs. (3) Barricading... environment. (8) Self-rescue and respiratory devices. The course shall include instruction and demonstration...

30 CFR 48.8 - Annual refresher training of miners; minimum courses of instruction; hours of instruction.

Code of Federal Regulations, 2012 CFR

2012-07-01

... controls and communication systems. The course shall include instruction on the procedures for riding on... the use of the mine communication systems, warning signals, and directional signs. (3) Barricading... environment. (8) Self-rescue and respiratory devices. The course shall include instruction and demonstration...

36 CFR 331.14 - Aircraft.

Code of Federal Regulations, 2010 CFR

2010-07-01

... GOVERNING THE PROTECTION, USE AND MANAGEMENT OF THE FALLS OF THE OHIO NATIONAL WILDLIFE CONSERVATION AREA, KENTUCKY AND INDIANA § 331.14 Aircraft. (a) The operation of aircraft on WCA lands and waters is prohibited... business of the Federal Government or used in emergency rescue in accordance with the directions of the...

46 CFR 108.560 - Rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Rescue boats. 108.560 Section 108.560 Shipping COAST... Lifesaving Equipment § 108.560 Rescue boats. Each unit must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.156. A lifeboat is accepted as a rescue boat if it also...

46 CFR 108.560 - Rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Rescue boats. 108.560 Section 108.560 Shipping COAST... Lifesaving Equipment § 108.560 Rescue boats. Each unit must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.156. A lifeboat is accepted as a rescue boat if it also...

30 CFR 49.7 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.7 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined...

30 CFR 49.7 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.7 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined...

30 CFR 49.7 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.7 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined...

46 CFR 10.109 - Classification of endorsements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... survival craft and rescue boats other than fast rescue boats (PSC). (16) Proficiency in survival craft and rescue boats other than fast rescue boats—limited (PSC—limited). (17) Proficiency in fast rescue boats...

Critical Endothelial Regulation by LRP5 during Retinal Vascular Development.

PubMed

Huang, Wei; Li, Qing; Amiry-Moghaddam, Mahmood; Hokama, Madoka; Sardi, Sylvia H; Nagao, Masashi; Warman, Matthew L; Olsen, Bjorn R

2016-01-01

Vascular abnormalities in the eye are the leading cause of many forms of inherited and acquired human blindness. Loss-of-function mutations in the Wnt-binding co-receptor LRP5 leads to aberrant ocular vascularization and loss of vision in genetic disorders such as osteoporosis-pseudoglioma syndrome. The canonical Wnt-β-catenin pathway is known to regulate retinal vascular development. However, it is unclear what precise role LPR5 plays in this process. Here, we show that loss of LRP5 function in mice causes retinal hypovascularization during development as well as retinal neovascularization in adulthood with disorganized and leaky vessels. Using a highly specific Flk1-CreBreier line for vascular endothelial cells, together with several genetic models, we demonstrate that loss of endothelium-derived LRP5 recapitulates the retinal vascular defects in Lrp5-/- mice. In addition, restoring LRP5 function only in endothelial cells in Lrp5-/- mice rescues their retinal vascular abnormalities. Furthermore, we show that retinal vascularization is regulated by LRP5 in a dosage dependent manner and does not depend on LRP6. Our study provides the first direct evidence that endothelium-derived LRP5 is both necessary and sufficient to mediate its critical role in the development and maintenance of retinal vasculature.

Critical Endothelial Regulation by LRP5 during Retinal Vascular Development

PubMed Central

Huang, Wei; Li, Qing; Amiry-Moghaddam, Mahmood; Hokama, Madoka; Sardi, Sylvia H.; Nagao, Masashi; Warman, Matthew L.; Olsen, Bjorn R.

2016-01-01

Vascular abnormalities in the eye are the leading cause of many forms of inherited and acquired human blindness. Loss-of-function mutations in the Wnt-binding co-receptor LRP5 leads to aberrant ocular vascularization and loss of vision in genetic disorders such as osteoporosis-pseudoglioma syndrome. The canonical Wnt-β-catenin pathway is known to regulate retinal vascular development. However, it is unclear what precise role LPR5 plays in this process. Here, we show that loss of LRP5 function in mice causes retinal hypovascularization during development as well as retinal neovascularization in adulthood with disorganized and leaky vessels. Using a highly specific Flk1-CreBreier line for vascular endothelial cells, together with several genetic models, we demonstrate that loss of endothelium-derived LRP5 recapitulates the retinal vascular defects in Lrp5-/- mice. In addition, restoring LRP5 function only in endothelial cells in Lrp5-/- mice rescues their retinal vascular abnormalities. Furthermore, we show that retinal vascularization is regulated by LRP5 in a dosage dependent manner and does not depend on LRP6. Our study provides the first direct evidence that endothelium-derived LRP5 is both necessary and sufficient to mediate its critical role in the development and maintenance of retinal vasculature. PMID:27031698

miR-958 inhibits Toll signaling and Drosomycin expression via direct targeting of Toll and Dif in Drosophila melanogaster.

PubMed

Li, Shengjie; Li, Yao; Shen, Li; Jin, Ping; Chen, Liming; Ma, Fei

2017-02-01

Drosophila melanogaster is widely used as a model system to study innate immunity and signaling pathways related to innate immunity, including the Toll signaling pathway. Although this pathway is well studied, the precise mechanisms of posttranscriptional regulation of key components of the Toll signaling pathway by microRNAs (miRNAs) remain obscure. In this study, we used an in silico strategy in combination with the Gal80 ts -Gal4 driver system to identify microRNA-958 (miR-958) as a candidate Toll pathway regulating miRNA in Drosophila We report that overexpression of miR-958 significantly reduces the expression of Drosomycin, a key antimicrobial peptide involved in Toll signaling and the innate immune response. We further demonstrate in vitro and in vivo that miR-958 targets the Toll and Dif genes, key components of the Toll signaling pathway, to negatively regulate Drosomycin expression. In addition, a miR-958 sponge rescued the expression of Toll and Dif, resulting in increased expression of Drosomycin. These results, not only revealed a novel function and modulation pattern of miR-958, but also provided a new insight into the underlying molecular mechanisms of Toll signaling in regulation of innate immunity. Copyright © 2017 the American Physiological Society.

Nicotinate-Curcumin Impedes Foam Cell Formation from THP-1 Cells through Restoring Autophagy Flux

PubMed Central

Gu, Hong-Feng; Li, Hai-Zhe; Tang, Ya-Ling; Tang, Xiao-Qing; Zheng, Xi-Long; Liao, Duan-Fang

2016-01-01

Our previous studies have indicated that a novel curcumin derivate nicotinate-curcumin (NC) has beneficial effects on the prevention of atherosclerosis, but the precise mechanisms are not fully understood. Given that autophagy regulates lipid metabolism, the present study was designed to investigate whether NC decreases foam cell formation through restoring autophagy flux in oxidized low-density lipoprotein (ox-LDL)-treated THP-1 cells. Our results showed that ox-LDL (100 μg/ml) was accumulated in THP-1 cells and impaired autophagy flux. Ox-LDL-induced impairment of autophagy was enhanced by treatment with the autophagy inhibitor chloroquine (CQ) and rescued by the autophagy inducer rapamycin. The aggregation of ox-LDL was increased by CQ, but decreased by rapamycin. In addition, colocalization of lipid droplets with LC3-II was remarkably reduced in ox-LDL group. In contrast, NC (10 μM) rescued the impaired autophagy flux by significantly increasing level of LC3-II, the number of autophagolysosomes, and the degradation of p62 in ox-LDL-treated THP-1 cells. Inhibition of the PI3K-Akt-mTOR signaling was required for NC-rescued autophagy flux. Notably, our results showed that NC remarkably promoted the colocalization of lipid droplets with autophagolysosomes, increased efflux of cholesterol, and reduced ox-LDL accumulation in THP-1 cells. However, treatment with 3-methyladenine (3-MA) or CQ reduced the protective effects of NC on lipid accumulation. Collectively, the findings suggest that NC decreases lipid accumulation in THP-1 cells through restoring autophagy flux, and further implicate that NC may be a potential therapeutic reagent to reverse atherosclerosis. PMID:27128486

46 CFR 133.140 - Stowage of rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Stowage of rescue boats. 133.140 Section 133.140... SYSTEMS Requirements for All OSVs § 133.140 Stowage of rescue boats. (a) Rescue boats must be stowed as follows: (1) Each rescue boat must be ready for launching in not more than 5 minutes. (2) Each rescue boat...

46 CFR 133.140 - Stowage of rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Stowage of rescue boats. 133.140 Section 133.140... SYSTEMS Requirements for All OSVs § 133.140 Stowage of rescue boats. (a) Rescue boats must be stowed as follows: (1) Each rescue boat must be ready for launching in not more than 5 minutes. (2) Each rescue boat...

46 CFR 133.140 - Stowage of rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Stowage of rescue boats. 133.140 Section 133.140... SYSTEMS Requirements for All OSVs § 133.140 Stowage of rescue boats. (a) Rescue boats must be stowed as follows: (1) Each rescue boat must be ready for launching in not more than 5 minutes. (2) Each rescue boat...

30 CFR 49.17 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.17 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a...

30 CFR 49.17 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.17 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a...

30 CFR 49.18 - Training for mine rescue teams.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Training for mine rescue teams. 49.18 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.18 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial...

30 CFR 49.8 - Training for mine rescue teams.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Training for mine rescue teams. 49.8 Section 49... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.8 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum...

30 CFR 49.17 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.17 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a...

30 CFR 49.8 - Training for mine rescue teams.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Training for mine rescue teams. 49.8 Section 49... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.8 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum...

30 CFR 49.18 - Training for mine rescue teams.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Training for mine rescue teams. 49.18 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.18 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial...

30 CFR 49.17 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.17 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a...

30 CFR 49.17 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.17 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a...

30 CFR 49.18 - Training for mine rescue teams.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Training for mine rescue teams. 49.18 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.18 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial...

30 CFR 49.18 - Training for mine rescue teams.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Training for mine rescue teams. 49.18 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.18 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial...

30 CFR 49.8 - Training for mine rescue teams.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Training for mine rescue teams. 49.8 Section 49... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.8 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum...

30 CFR 49.18 - Training for mine rescue teams.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Training for mine rescue teams. 49.18 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.18 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial...

Assessing the efficacy of rescue equipment in lifeguard resuscitation efforts for drowning.

PubMed

Barcala-Furelos, Roberto; Szpilman, David; Palacios-Aguilar, Jose; Costas-Veiga, Javier; Abelairas-Gomez, Cristian; Bores-Cerezal, Antonio; López-García, Sergio; Rodríguez-Nuñez, Antonio

2016-03-01

The whole drowning process usually occurs within seconds to a few minutes. An early rescue may stop and/or prevent most medical complications. Fins, rescue tube, and rescue board (RB) are the equipment most frequently used by lifeguards. Our objective was to compare, in a water rescue quasiexperimental trial, these different pieces of rescue equipment to define the safest and with the lower rescue time as well as to assess their effects on the lifeguards' physiological state and cardiopulmonary resuscitation (CPR) performance. A controlled trial was conducted to study the time effect of 4 different rescue techniques and assess CPR quality, along with the physiological effects of each rescue technique (blood lactate and subjective Borg's scale effort perception) on 35 lifeguards. Among the final sample subjects (n = 23), a total of 92 rescues were completed. Total water rescue time was longer without equipment (NE). The total rescue time was significantly lower using RB (P < .001). Similar good quality of CPR before and after water rescue was observed in all trials (P > .05), although correct ventilations represented less than 50% of total in all trials. Blood lactate increased after all rescues. The subjective effort Borg's scale showed significantly less effort using RB vs without equipment, fins, and fins and rescue tube. The use of propelling and/or floating equipment saves precious time with repercussions in the reduction of drowning mortality and morbidity. The RB offers a significant advantage. Lifeguards need more CPR training, especially considering the importance of efficient ventilations for drowning victims. Copyright © 2015 Elsevier Inc. All rights reserved.

Helicopter Mountain Rescue in Slovenia from 2011 to 2015.

PubMed

Deeb, Janina Golob; Walter, Nicholas; Carrico, Caroline; Gašperin, Miha; Deeb, George R

2018-03-01

The popularity of adventure recreation in wilderness areas across the world continues to increase. Nevertheless, the risk of injury and illness remains significant. The purpose of this study is to analyze the mountain rescue operations performed in Slovenia between 2011 and 2015. This retrospective study reports mountain rescue operations documented by the Slovenian National Mountain Rescue Association. The annual number of ground-based and helicopter-based rescues were identified and compared. For 2015, the indication for rescue and the severity of injury were also analyzed, specifically for interventions requiring the use of a helicopter. From 2011 through 2015, the number of rescues remained consistent with an annual average of 413 (SD ±15; range, 393-434) rescues. However, the percentage of ground-based rescues varied significantly year by year (P=0.016), with highest rate in 2014 (68%) and the lowest in 2015 (56%). In 2015, 434 mountain rescue operations were reported in Slovenia. Injury accounted for 44%, illness for 10%, and fatality for 9% of the rescues. In 37%, no illness or injury was reported. Helicopter rescue was used in 190 (44%) of all interventions. Among the 190 helicopter rescues, 49% of patients had nonfatal injuries, 29% required no medical treatment, 15% had illness, and 7% had fatal injuries. A significant number of mountain rescue operations were conducted in Slovenia from 2011 through 2015. Most of these were needed for injured, ill, or deceased persons. A notable number of rescues in 2015 required a helicopter. Copyright © 2017 Wilderness Medical Society. Published by Elsevier Inc. All rights reserved.

33 CFR 149.314 - What are the approval and stowage requirements for rescue boats?

Code of Federal Regulations, 2010 CFR

2010-07-01

... requirements for rescue boats? 149.314 Section 149.314 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... requirements for rescue boats? (a) Rescue boats must be approved under approval series 160.156. A lifeboat is acceptable as a rescue boat if it also meets the requirements for a rescue boat under approval series 160.156...

33 CFR 149.314 - What are the approval and stowage requirements for rescue boats?

Code of Federal Regulations, 2013 CFR

2013-07-01

... requirements for rescue boats? 149.314 Section 149.314 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... requirements for rescue boats? (a) Rescue boats must be approved under approval series 160.156. A lifeboat is acceptable as a rescue boat if it also meets the requirements for a rescue boat under approval series 160.156...

33 CFR 149.314 - What are the approval and stowage requirements for rescue boats?

Code of Federal Regulations, 2012 CFR

2012-07-01

... requirements for rescue boats? 149.314 Section 149.314 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... requirements for rescue boats? (a) Rescue boats must be approved under approval series 160.156. A lifeboat is acceptable as a rescue boat if it also meets the requirements for a rescue boat under approval series 160.156...

33 CFR 149.314 - What are the approval and stowage requirements for rescue boats?

Code of Federal Regulations, 2014 CFR

2014-07-01

... requirements for rescue boats? 149.314 Section 149.314 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... requirements for rescue boats? (a) Rescue boats must be approved under approval series 160.156. A lifeboat is acceptable as a rescue boat if it also meets the requirements for a rescue boat under approval series 160.156...

33 CFR 149.314 - What are the approval and stowage requirements for rescue boats?

Code of Federal Regulations, 2011 CFR

2011-07-01

... requirements for rescue boats? 149.314 Section 149.314 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... requirements for rescue boats? (a) Rescue boats must be approved under approval series 160.156. A lifeboat is acceptable as a rescue boat if it also meets the requirements for a rescue boat under approval series 160.156...

46 CFR 12.615 - Requirements to qualify for an STCW endorsement in proficiency in survival craft and rescue boats...

Code of Federal Regulations, 2014 CFR

2014-10-01

... proficiency in survival craft and rescue boats other than lifeboats and fast rescue boats-limited (PSC-limited... lifeboats and fast rescue boats-limited (PSC-limited). (a) To qualify for an STCW endorsement in proficiency in survival craft and rescue boats other than lifeboats and fast rescue boats-limited (PSC-limited...

Lunar mission safety and rescue: Escape/rescue analysis and plan

NASA Technical Reports Server (NTRS)

1971-01-01

The results are presented of the technical analysis of escape/rescue/survival situations, crew survival techniques, alternate escape/rescue approaches and vehicles, and the advantages and disadvantages of each for advanced lunar exploration. Candidate escape/rescue guidelines are proposed and elements of a rescue plan developed. The areas of discussions include the following: lunar arrival/departure operations, lunar orbiter operations, lunar surface operations, lunar surface base escape/rescue analysis, lander tug location operations, portable airlock, emergency pressure suit, and the effects of no orbiting lunar station, no lunar surface base, and no foreign lunar orbit/surface operations on the escape/rescue plan.

Validating precision estimates in horizontal wind measurements from a Doppler lidar

DOE PAGES

Newsom, Rob K.; Brewer, W. Alan; Wilczak, James M.; ...

2017-03-30

Results from a recent field campaign are used to assess the accuracy of wind speed and direction precision estimates produced by a Doppler lidar wind retrieval algorithm. The algorithm, which is based on the traditional velocity-azimuth-display (VAD) technique, estimates the wind speed and direction measurement precision using standard error propagation techniques, assuming the input data (i.e., radial velocities) to be contaminated by random, zero-mean, errors. For this study, the lidar was configured to execute an 8-beam plan-position-indicator (PPI) scan once every 12 min during the 6-week deployment period. Several wind retrieval trials were conducted using different schemes for estimating themore » precision in the radial velocity measurements. Here, the resulting wind speed and direction precision estimates were compared to differences in wind speed and direction between the VAD algorithm and sonic anemometer measurements taken on a nearby 300 m tower.« less

The earthquake disaster risk characteristic and the problem in the earthquake emergency rescue of mountainous southwestern Sichuan

NASA Astrophysics Data System (ADS)

Yuan, S.; Xin, C.; Ying, Z.

2016-12-01

In recent years, earthquake disaster occurred frequently in Chinese mainland, the secondary disaster which have been caused by it is more serious in mountainous region. Because of the influence of terrain and geological conditions, the difficulty of earthquake emergency rescue work greatly increased, rescue force is also urged. Yet, it has been studied less on earthquake emergency rescue in mountainous region, the research in existing equipment whether can meet the actual needs of local earthquake emergency rescue is poorly. This paper intends to discuss and solve these problems. Through the mountainous regions Ganzi and Liangshan states in Sichuan field research, we investigated the process of earthquake emergency response and the projects for rescue force after an earthquake, and we also collected and collated local rescue force based data. By consulting experts and statistical analyzing the basic data, there are mainly two problems: The first is about local rescue force, they are poorly equipped and lack in the knowledge of medical help or identify architectural structure. There are no countries to establish a sound financial investment protection mechanism. Also, rescue equipment's updates and maintenance; The second problem is in earthquake emergency rescue progress. In the complicated geologic structure of mountainous regions, traffic and communication may be interrupted by landslides and mud-rock flows after earthquake. The outside rescue force may not arrive in time, rescue equipment was transported by manpower. Because of unknown earthquake disaster information, the local rescue force was deployed unreasonable. From the above, the local government worker should analyze the characteristics of the earthquake disaster in mountainous regions, and research how to improve their earthquake emergency rescue ability. We think they can do that by strengthening and regulating the rescue force structure, enhancing the skills and knowledge, training rescue workers, outfitting the light and portable rescue equipment, improving the public's self and mutual aid ability. All these measures will help local government reach the final goal of reducing the earthquake disaster.

Fatal Attraction of Short-Tailed Shearwaters to Artificial Lights

PubMed Central

Rodríguez, Airam; Burgan, Graeme; Dann, Peter; Jessop, Roz; Negro, Juan J.; Chiaradia, Andre

2014-01-01

Light pollution is increasing around the world and altering natural nightscapes with potential ecological and evolutionary consequences. A severe ecological perturbation caused by artificial lights is mass mortalities of organisms, including seabird fledglings that are attracted to lights at night on their first flights to the sea. Here, we report on the number of fledging short-tailed shearwaters Ardenna tenuirostris found grounded in evening and morning rescue patrols conducted at Phillip Island, Australia, during a 15-year period (1999–2013). We assessed factors affecting numbers of grounded birds and mortality including date, moon phase, wind direction and speed, number of visitors and holiday periods. We also tested experimentally if birds were attracted to lights by turning the lights off on a section of the road. Of 8871 fledglings found, 39% were dead or dying. This mortality rate was 4–8 times higher than reported elsewhere for other shearwater species, probably because searching for fledglings was part of our systematic rescue effort rather than the opportunistic rescue used elsewhere. Thus, it suggests that light-induced mortality of seabirds is usually underestimated. We rescued more birds (dead and alive) in peak fledging, moonless and windy nights. Mortality increased through the fledging period, in the mornings and with increased traffic on holiday periods. Turning the road lights off decreased the number of grounded birds (dead and alive). While moon, wind and time are uncontrolled natural constraints, we demonstrated that reduction of light pollution and better traffic management can mitigate artificial light-induced mortality. PMID:25334014

46 CFR 12.613 - Requirements to qualify for an STCW endorsement in proficiency in survival craft and rescue boats...

Code of Federal Regulations, 2014 CFR

2014-10-01

... proficiency in survival craft and rescue boats other than fast rescue boats (PSC). 12.613 Section 12.613... STCW endorsement in proficiency in survival craft and rescue boats other than fast rescue boats (PSC... fast rescue boats (PSC), the applicant must— (1) Be at least 18 years of age; (2) Meet the requirements...

47 CFR 80.1125 - Search and rescue coordinating communications.

Code of Federal Regulations, 2011 CFR

2011-10-01

... station involved may impose silence on stations which interfere with that traffic. This instruction may be... “silence, m'aider”; (2) In narrow-band direct-printing telegraphy normally using forward-error correcting mode, the signal SILENCE MAYDAY. However, the ARQ mode may be used when it is advantageous to do so. (f...

To direct the Administrator of the Federal Emergency Management Agency to designate New Jersey Task Force 1 as part of the National Urban Search and Rescue System.

THOMAS, 113th Congress

Rep. Frelinghuysen, Rodney P. [R-NJ-11

2013-05-22

House - 05/23/2013 Referred to the Subcommittee on Economic Development, Public Buildings and Emergency Management. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Natural Speech Toward Humans and Intelligent Agents During a Simulated Search and Rescue Mission

DTIC Science & Technology

2008-12-01

Eklundh, 2006). Research has been done on giving directions to robots, and the point of view that teammates normally attribute to them (Imai, Hiraki ...Bystander intervention as a resource in human-robot collaboration. Interaction Studies, 7(3), 455-477. Imai, M., Hiraki , K., Miyasato, T., Nakatsu, R

miR-11 regulates pupal size of Drosophila melanogaster via directly targeting Ras85D.

PubMed

Li, Yao; Li, Shengjie; Jin, Ping; Chen, Liming; Ma, Fei

2017-01-01

MicroRNAs play diverse roles in various physiological processes during Drosophila development. In the present study, we reported that miR-11 regulates pupal size during Drosophila metamorphosis via targeting Ras85D with the following evidences: pupal size was increased in the miR-11 deletion mutant; restoration of miR-11 in the miR-11 deletion mutant rescued the increased pupal size phenotype observed in the miR-11 deletion mutant; ectopic expression of miR-11 in brain insulin-producing cells (IPCs) and whole body shows consistent alteration of pupal size; Dilps and Ras85D expressions were negatively regulated by miR-11 in vivo; miR-11 targets Ras85D through directly binding to Ras85D 3'-untranslated region in vitro; removal of one copy of Ras85D in the miR-11 deletion mutant rescued the increased pupal size phenotype observed in the miR-11 deletion mutant. Thus, our current work provides a novel mechanism of pupal size determination by microRNAs during Drosophila melanogaster metamorphosis. Copyright © 2017 the American Physiological Society.

Modulation of individual steps in group I intron catalysis by a peripheral metal ion.

PubMed

Forconi, Marcello; Piccirilli, Joseph A; Herschlag, Daniel

2007-10-01

Enzymes are complex macromolecules that catalyze chemical reactions at their active sites. Important information about catalytic interactions is commonly gathered by perturbation or mutation of active site residues that directly contact substrates. However, active sites are engaged in intricate networks of interactions within the overall structure of the macromolecule, and there is a growing body of evidence about the importance of peripheral interactions in the precise structural organization of the active site. Here, we use functional studies, in conjunction with published structural information, to determine the effect of perturbation of a peripheral metal ion binding site on catalysis in a well-characterized catalytic RNA, the Tetrahymena thermophila group I ribozyme. We perturbed the metal ion binding site by site-specifically introducing a phosphorothioate substitution in the ribozyme's backbone, replacing the native ligands (the pro-R (P) oxygen atoms at positions 307 and 308) with sulfur atoms. Our data reveal that these perturbations affect several reaction steps, including the chemical step, despite the absence of direct contacts of this metal ion with the atoms involved in the chemical transformation. As structural probing with hydroxyl radicals did not reveal significant change in the three-dimensional structure upon phosphorothioate substitution, the effects are likely transmitted through local, rather subtle conformational rearrangements. Addition of Cd(2+), a thiophilic metal ion, rescues some reaction steps but has deleterious effects on other steps. These results suggest that native interactions in the active site may have been aligned by the naturally occurring peripheral residues and interactions to optimize the overall catalytic cycle.

Sustaining Statewide Disaster Response Capabilities from a Fire Service Perspective

DTIC Science & Technology

2013-03-01

2010). These increased capabilities can be vital to the success of response operations that involve automobile accidents, train wrecks , boat...Trench Rescue  Subterranean Rescue  Dive Rescue  Wilderness Rescue 2. 1670 Operations and Training for Technical Search and Rescue Incidents The

The 4Rs of Aquatic Rescue: educating the public about safety and risks of bystander rescue.

PubMed

Moran, Kevin; Webber, Jonathon; Stanley, Teresa

2017-09-01

From 1980 to 2014, 87 persons drowned in New Zealand while attempting to rescue others; all incidents occurred in open water and most (80%) fatalities were male. While bystander rescue has been promoted as a way of preventing drowning, little is known about the knowledge base that informs potential rescuers. This study utilized a family water safety programme to promote a resource entitled the 4Rs of Aquatic Rescue. Participants (n = 174) completed a pre-intervention survey and were then provided with information and access to electronic resources on safe bystander rescue techniques. Most respondents (71%) had never been taught rescue techniques, and males were more confident of their rescue ability. Upon completion of the programme, significant differences were evident in respondents' understanding of rescue safety, but this did not translate to greater confidence or disposition towards performing a rescue. Ways of promoting bystander safety around water are discussed and recommendations for future studies are made.

Evolutionary rescue in vertebrates: evidence, applications and uncertainty

PubMed Central

Vander Wal, E.; Garant, D.; Festa-Bianchet, M.; Pelletier, F.

2013-01-01

The current rapid rate of human-driven environmental change presents wild populations with novel conditions and stresses. Theory and experimental evidence for evolutionary rescue present a promising case for species facing environmental change persisting via adaptation. Here, we assess the potential for evolutionary rescue in wild vertebrates. Available information on evolutionary rescue was rare and restricted to abundant and highly fecund species that faced severe intentional anthropogenic selective pressures. However, examples from adaptive tracking in common species and genetic rescues in species of conservation concern provide convincing evidence in favour of the mechanisms of evolutionary rescue. We conclude that low population size, long generation times and limited genetic variability will result in evolutionary rescue occurring rarely for endangered species without intervention. Owing to the risks presented by current environmental change and the possibility of evolutionary rescue in nature, we suggest means to study evolutionary rescue by mapping genotype → phenotype → demography → fitness relationships, and priorities for applying evolutionary rescue to wild populations. PMID:23209171

Rescue Manual. Module 6.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. Instructional Materials Lab.

This learner manual for rescuers covers the current techniques or practices required in the rescue service. The sixth of 10 modules contains 4 chapters: (1) industrial rescue; (2) rescue from a confined space; (3) extrication from heavy equipment; and (4) rescue operations involving elevators. Key points, an introduction, and conclusion accompany…

Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes.

PubMed

Matsa, Elena; Dixon, James E; Medway, Christopher; Georgiou, Orestis; Patel, Minal J; Morgan, Kevin; Kemp, Paul J; Staniforth, Andrew; Mellor, Ian; Denning, Chris

2014-04-01

Long-QT syndromes (LQTS) are mostly autosomal-dominant congenital disorders associated with a 1:1000 mutation frequency, cardiac arrest, and sudden death. We sought to use cardiomyocytes derived from human-induced pluripotency stem cells (hiPSCs) as an in vitro model to develop and evaluate gene-based therapeutics for the treatment of LQTS. We produced LQTS-type 2 (LQT2) hiPSC cardiomyocytes carrying a KCNH2 c.G1681A mutation in a IKr ion-channel pore, which caused impaired glycosylation and channel transport to cell surface. Allele-specific RNA interference (RNAi) directed towards the mutated KCNH2 mRNA caused knockdown, while leaving the wild-type mRNA unaffected. Electrophysiological analysis of patient-derived LQT2 hiPSC cardiomyocytes treated with mutation-specific siRNAs showed normalized action potential durations (APDs) and K(+) currents with the concurrent rescue of spontaneous and drug-induced arrhythmias (presented as early-afterdepolarizations). These findings provide in vitro evidence that allele-specific RNAi can rescue diseased phenotype in LQTS cardiomyocytes. This is a potentially novel route for the treatment of many autosomal-dominant-negative disorders, including those of the heart.

Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes

PubMed Central

Matsa, Elena; Dixon, James E.; Medway, Christopher; Georgiou, Orestis; Patel, Minal J.; Morgan, Kevin; Kemp, Paul J.; Staniforth, Andrew; Mellor, Ian; Denning, Chris

2014-01-01

Aims Long-QT syndromes (LQTS) are mostly autosomal-dominant congenital disorders associated with a 1:1000 mutation frequency, cardiac arrest, and sudden death. We sought to use cardiomyocytes derived from human-induced pluripotency stem cells (hiPSCs) as an in vitro model to develop and evaluate gene-based therapeutics for the treatment of LQTS. Methods and results We produced LQTS-type 2 (LQT2) hiPSC cardiomyocytes carrying a KCNH2 c.G1681A mutation in a IKr ion-channel pore, which caused impaired glycosylation and channel transport to cell surface. Allele-specific RNA interference (RNAi) directed towards the mutated KCNH2 mRNA caused knockdown, while leaving the wild-type mRNA unaffected. Electrophysiological analysis of patient-derived LQT2 hiPSC cardiomyocytes treated with mutation-specific siRNAs showed normalized action potential durations (APDs) and K+ currents with the concurrent rescue of spontaneous and drug-induced arrhythmias (presented as early-afterdepolarizations). Conclusions These findings provide in vitro evidence that allele-specific RNAi can rescue diseased phenotype in LQTS cardiomyocytes. This is a potentially novel route for the treatment of many autosomal-dominant-negative disorders, including those of the heart. PMID:23470493

46 CFR 108.560 - Rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Rescue boats. 108.560 Section 108.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.560 Rescue boats. Each unit must carry at least one rescue boat. Each rescue boat...

46 CFR 108.560 - Rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Rescue boats. 108.560 Section 108.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.560 Rescue boats. Each unit must carry at least one rescue boat. Each rescue boat...

46 CFR 108.560 - Rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Rescue boats. 108.560 Section 108.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.560 Rescue boats. Each unit must carry at least one rescue boat. Each rescue boat...

46 CFR 199.262 - Rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Rescue boats. 199.262 Section 199.262 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Cargo Vessels § 199.262 Rescue boats. (a) Each cargo vessel must carry at least one rescue boat. Each rescue boat must be approved under approval...

46 CFR 199.262 - Rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Rescue boats. 199.262 Section 199.262 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Cargo Vessels § 199.262 Rescue boats. (a) Each cargo vessel must carry at least one rescue boat. Each rescue boat must be approved under approval...

46 CFR 199.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Rescue boat embarkation, launching and recovery... Vessels § 199.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each rescue boat must... be used to meet this requirement. (b) Each rescue boat embarkation and launching arrangement must...

46 CFR 199.262 - Rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Rescue boats. 199.262 Section 199.262 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Cargo Vessels § 199.262 Rescue boats. (a) Each cargo vessel must carry at least one rescue boat. Each rescue boat must be approved under approval...

46 CFR 199.262 - Rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Rescue boats. 199.262 Section 199.262 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Cargo Vessels § 199.262 Rescue boats. (a) Each cargo vessel must carry at least one rescue boat. Each rescue boat must be approved under approval...

46 CFR 199.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Rescue boat embarkation, launching and recovery... Vessels § 199.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each rescue boat must... be used to meet this requirement. (b) Each rescue boat embarkation and launching arrangement must...

46 CFR 199.262 - Rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Rescue boats. 199.262 Section 199.262 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Cargo Vessels § 199.262 Rescue boats. (a) Each cargo vessel must carry at least one rescue boat. Each rescue boat must be approved under approval...

46 CFR 199.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Rescue boat embarkation, launching and recovery... Vessels § 199.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each rescue boat must... be used to meet this requirement. (b) Each rescue boat embarkation and launching arrangement must...

46 CFR 199.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Rescue boat embarkation, launching and recovery... Vessels § 199.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each rescue boat must... be used to meet this requirement. (b) Each rescue boat embarkation and launching arrangement must...

46 CFR 199.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Rescue boat embarkation, launching and recovery... Vessels § 199.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each rescue boat must... be used to meet this requirement. (b) Each rescue boat embarkation and launching arrangement must...

30 CFR 49.5 - Mine rescue station.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Mine rescue station. 49.5 Section 49.5 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.5 Mine rescue station. (a) Except...

30 CFR 49.15 - Mine rescue station.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Mine rescue station. 49.15 Section 49.15 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.15 Mine rescue station. (a) Every operator...

30 CFR 49.15 - Mine rescue station.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Mine rescue station. 49.15 Section 49.15 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.15 Mine rescue station. (a) Every operator...

30 CFR 49.5 - Mine rescue station.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Mine rescue station. 49.5 Section 49.5 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.5 Mine rescue station. (a) Except...

30 CFR 49.15 - Mine rescue station.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Mine rescue station. 49.15 Section 49.15 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.15 Mine rescue station. (a) Every operator...

30 CFR 49.5 - Mine rescue station.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Mine rescue station. 49.5 Section 49.5 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.5 Mine rescue station. (a) Except...

30 CFR 49.15 - Mine rescue station.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Mine rescue station. 49.15 Section 49.15 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.15 Mine rescue station. (a) Every operator...

30 CFR 49.15 - Mine rescue station.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Mine rescue station. 49.15 Section 49.15 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.15 Mine rescue station. (a) Every operator...

46 CFR 15.404 - Requirements for serving onboard a vessel.

Code of Federal Regulations, 2014 CFR

2014-10-01

... rescue boats other than fast rescue boats (PSC). (f) Lifeboatman-limited. Every person assigned duties... boats other than lifeboats and fast rescue boats—limited (PSC—limited). (g) Fast rescue boats. Every person engaged or employed in a position requiring proficiency in fast rescue boats must hold an...

46 CFR 108.570 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2012 CFR

2012-10-01

...-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.570 Rescue boat... possible time. (c) If the rescue boat is one of the unit's survival craft, the rescue boat must also be as... approval series 160.170, instead of a lifeboat release mechanism. (d) Rapid recovery of the rescue boat...

46 CFR 108.570 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2013 CFR

2013-10-01

...-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.570 Rescue boat... possible time. (c) If the rescue boat is one of the unit's survival craft, the rescue boat must also be as... approval series 160.170, instead of a lifeboat release mechanism. (d) Rapid recovery of the rescue boat...

46 CFR 108.570 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2011 CFR

2011-10-01

...-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.570 Rescue boat... possible time. (c) If the rescue boat is one of the unit's survival craft, the rescue boat must also be as... approval series 160.170, instead of a lifeboat release mechanism. (d) Rapid recovery of the rescue boat...

46 CFR 108.570 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2014 CFR

2014-10-01

...-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.570 Rescue boat... possible time. (c) If the rescue boat is one of the unit's survival craft, the rescue boat must also be as... approval series 160.170, instead of a lifeboat release mechanism. (d) Rapid recovery of the rescue boat...

46 CFR 108.570 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2010 CFR

2010-10-01

...-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.570 Rescue boat... possible time. (c) If the rescue boat is one of the unit's survival craft, the rescue boat must also be as... approval series 160.170, instead of a lifeboat release mechanism. (d) Rapid recovery of the rescue boat...

33 CFR 149.315 - What embarkation, launching, and recovery arrangements must rescue boats meet?

Code of Federal Regulations, 2013 CFR

2013-07-01

... recovery arrangements must rescue boats meet? 149.315 Section 149.315 Navigation and Navigable Waters COAST..., launching, and recovery arrangements must rescue boats meet? (a) Each rescue boat must be capable of being... boat embarkation and launching arrangement must permit the rescue boat to be boarded and launched in...

46 CFR 160.056-4 - Approval tests of prototype rescue boat.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 6 2011-10-01 2011-10-01 false Approval tests of prototype rescue boat. 160.056-4..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boat § 160.056-4 Approval tests of prototype rescue boat. (a) Drop test. The rescue boat, fully equipped, shall be dropped, in a...

46 CFR 160.056-4 - Approval tests of prototype rescue boat.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 6 2012-10-01 2012-10-01 false Approval tests of prototype rescue boat. 160.056-4..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boat § 160.056-4 Approval tests of prototype rescue boat. (a) Drop test. The rescue boat, fully equipped, shall be dropped, in a...

46 CFR 108.565 - Stowage of rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Stowage of rescue boats. 108.565 Section 108.565... AND EQUIPMENT Lifesaving Equipment § 108.565 Stowage of rescue boats. (a) Rescue boats must be stowed as follows: (1) Each rescue boat must be ready for launching in not more than 5 minutes. (2) Each...

46 CFR 160.056-4 - Approval tests of prototype rescue boat.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 6 2014-10-01 2014-10-01 false Approval tests of prototype rescue boat. 160.056-4..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boat § 160.056-4 Approval tests of prototype rescue boat. (a) Drop test. The rescue boat, fully equipped, shall be dropped, in a...

46 CFR 108.565 - Stowage of rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Stowage of rescue boats. 108.565 Section 108.565... AND EQUIPMENT Lifesaving Equipment § 108.565 Stowage of rescue boats. (a) Rescue boats must be stowed as follows: (1) Each rescue boat must be ready for launching in not more than 5 minutes. (2) Each...

46 CFR 160.056-4 - Approval tests of prototype rescue boat.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 6 2013-10-01 2013-10-01 false Approval tests of prototype rescue boat. 160.056-4..., CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boat § 160.056-4 Approval tests of prototype rescue boat. (a) Drop test. The rescue boat, fully equipped, shall be dropped, in a...

30 CFR 49.12 - Availability of mine rescue teams.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Availability of mine rescue teams. 49.12... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.12 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines (§ 49.13), every...

30 CFR 49.2 - Availability of mine rescue teams.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Availability of mine rescue teams. 49.2 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.2 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines...

30 CFR 49.12 - Availability of mine rescue teams.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Availability of mine rescue teams. 49.12... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.12 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines (§ 49.13), every...

30 CFR 49.12 - Availability of mine rescue teams.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Availability of mine rescue teams. 49.12... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.12 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines (§ 49.13), every...

30 CFR 49.12 - Availability of mine rescue teams.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Availability of mine rescue teams. 49.12... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.12 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines (§ 49.13), every...

30 CFR 49.12 - Availability of mine rescue teams.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Availability of mine rescue teams. 49.12... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.12 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines (§ 49.13), every...

30 CFR 49.2 - Availability of mine rescue teams.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Availability of mine rescue teams. 49.2 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.2 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines...

30 CFR 49.2 - Availability of mine rescue teams.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Availability of mine rescue teams. 49.2 Section... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.2 Availability of mine rescue teams. (a) Except where alternative compliance is permitted for small and remote mines...

46 CFR 12.601 - General requirements for STCW rating endorsements.

Code of Federal Regulations, 2014 CFR

2014-10-01

...,000 HP or more. (6) Proficiency in survival craft and rescue boats, other than fast rescue boats (PSC). (7) Proficiency in survival craft and rescue boats, other than lifeboats and fast rescue boats (PSC-limited). (8) Proficiency in fast rescue boats. (9) Medical first-aid provider. (10) Person in charge of...

Failure-to-rescue after injury is associated with preventability: The results of mortality panel review of failure-to-rescue cases in trauma.

PubMed

Kuo, Lindsay E; Kaufman, Elinore; Hoffman, Rebecca L; Pascual, Jose L; Martin, Niels D; Kelz, Rachel R; Holena, Daniel N

2017-03-01

Failure-to-rescue is defined as the conditional probability of death after a complication, and the failure-to-rescue rate reflects a center's ability to successfully "rescue" patients after complications. The validity of the failure-to-rescue rate as a quality measure is dependent on the preventability of death and the appropriateness of this measure for use in the trauma population is untested. We sought to evaluate the relationship between preventability and failure-to-rescue in trauma. All adjudications from a mortality review panel at an academic level I trauma center from 2005-2015 were merged with registry data for the same time period. The preventability of each death was determined by panel consensus as part of peer review. Failure-to-rescue deaths were defined as those occurring after any registry-defined complication. Univariate and multivariate logistic regression models between failure-to-rescue status and preventability were constructed and time to death was examined using survival time analyses. Of 26,557 patients, 2,735 (10.5%) had a complication, of whom 359 died for a failure-to-rescue rate of 13.2%. Of failure-to-rescue deaths, 272 (75.6%) were judged to be non-preventable, 65 (18.1%) were judged potentially preventable, and 22 (6.1%) were judged to be preventable by peer review. After adjusting for other patient factors, there remained a strong association between failure-to-rescue status and potentially preventable (odds ratio 2.32, 95% confidence interval, 1.47-3.66) and preventable (odds ratio 14.84, 95% confidence interval, 3.30-66.71) judgment. Despite a strong association between failure-to-rescue status and preventability adjudication, only a minority of deaths meeting the definition of failure to rescue were judged to be preventable or potentially preventable. Revision of the failure-to-rescue metric before use in trauma care benchmarking is warranted. Copyright © 2016 Elsevier Inc. All rights reserved.

33 CFR 150.511 - What are the operational testing requirements for lifeboat and rescue boat release gear?

Code of Federal Regulations, 2010 CFR

2010-07-01

... requirements for lifeboat and rescue boat release gear? 150.511 Section 150.511 Navigation and Navigable Waters... operational testing requirements for lifeboat and rescue boat release gear? (a) Lifeboat and rescue boat... rescue boat when loaded with its full complement of persons and equipment. (b) The test must be conducted...

33 CFR 150.511 - What are the operational testing requirements for lifeboat and rescue boat release gear?

Code of Federal Regulations, 2011 CFR

2011-07-01

... requirements for lifeboat and rescue boat release gear? 150.511 Section 150.511 Navigation and Navigable Waters... operational testing requirements for lifeboat and rescue boat release gear? (a) Lifeboat and rescue boat... rescue boat when loaded with its full complement of persons and equipment. (b) The test must be conducted...

33 CFR 150.511 - What are the operational testing requirements for lifeboat and rescue boat release gear?

Code of Federal Regulations, 2014 CFR

2014-07-01

... requirements for lifeboat and rescue boat release gear? 150.511 Section 150.511 Navigation and Navigable Waters... operational testing requirements for lifeboat and rescue boat release gear? (a) Lifeboat and rescue boat... rescue boat when loaded with its full complement of persons and equipment. (b) The test must be conducted...

46 CFR 133.135 - Rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Rescue boats. 133.135 Section 133.135 Shipping COAST... Requirements for All OSVs § 133.135 Rescue boats. (a) Each OSV must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.156 and equipped as specified in table 133.175 of this...

46 CFR 133.135 - Rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Rescue boats. 133.135 Section 133.135 Shipping COAST... Requirements for All OSVs § 133.135 Rescue boats. (a) Each OSV must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.156 and equipped as specified in table 133.175 of this...

33 CFR 150.511 - What are the operational testing requirements for lifeboat and rescue boat release gear?

Code of Federal Regulations, 2012 CFR

2012-07-01

... requirements for lifeboat and rescue boat release gear? 150.511 Section 150.511 Navigation and Navigable Waters... operational testing requirements for lifeboat and rescue boat release gear? (a) Lifeboat and rescue boat... rescue boat when loaded with its full complement of persons and equipment. (b) The test must be conducted...

46 CFR 133.135 - Rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Rescue boats. 133.135 Section 133.135 Shipping COAST... Requirements for All OSVs § 133.135 Rescue boats. (a) Each OSV must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.056 and equipped as specified in table 133.175 of this...

46 CFR 133.135 - Rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Rescue boats. 133.135 Section 133.135 Shipping COAST... Requirements for All OSVs § 133.135 Rescue boats. (a) Each OSV must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.156 and equipped as specified in table 133.175 of this...

33 CFR 150.511 - What are the operational testing requirements for lifeboat and rescue boat release gear?

Code of Federal Regulations, 2013 CFR

2013-07-01

... requirements for lifeboat and rescue boat release gear? 150.511 Section 150.511 Navigation and Navigable Waters... operational testing requirements for lifeboat and rescue boat release gear? (a) Lifeboat and rescue boat... rescue boat when loaded with its full complement of persons and equipment. (b) The test must be conducted...

46 CFR 133.135 - Rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Rescue boats. 133.135 Section 133.135 Shipping COAST... Requirements for All OSVs § 133.135 Rescue boats. (a) Each OSV must carry at least one rescue boat. Each rescue boat must be approved under approval series 160.156 and equipped as specified in table 133.175 of this...

30 CFR 49.7 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.7 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a physician who shall certify that each person is...

30 CFR 49.8 - Training for mine rescue teams.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Training for mine rescue teams. 49.8 Section 49... TRAINING MINE RESCUE TEAMS § 49.8 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial 20-hour course of instruction as prescribed...

30 CFR 49.7 - Physical requirements for mine rescue team.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Physical requirements for mine rescue team. 49... EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.7 Physical requirements for mine rescue team. (a) Each member of a mine rescue team shall be examined annually by a physician who shall certify that each person is...

30 CFR 49.8 - Training for mine rescue teams.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Training for mine rescue teams. 49.8 Section 49... TRAINING MINE RESCUE TEAMS § 49.8 Training for mine rescue teams. (a) Prior to serving on a mine rescue team each member shall complete, at a minimum, an initial 20-hour course of instruction as prescribed...

46 CFR 12.603 - Requirements to qualify for an STCW endorsement as able seafarer-deck.

Code of Federal Regulations, 2014 CFR

2014-10-01

... satisfactorily completed approved training in— (i) Proficiency in survival craft and rescue boats other than fast rescue boats (PSC); or (ii) Proficiency in survival craft and rescue boats, other than lifeboats or fast... completed approved training in— (i) Proficiency in survival craft and rescue boats, other than fast rescue...

The 1983 Amendments to the Social Security Act: The Issue of Intergenerational Equity.

ERIC Educational Resources Information Center

Ozawa, Martha N.

1984-01-01

Reviews the conditions that prevailed before the passage of the 1983 amendments to the Social Security Act, the probable effects of the amendments, and the future direction of social security. Questions whether the amendments have in reality rescued social security from financial insolvency and discusses the concept of intergenerational equity.…

A Precision Medicine Approach to the Rescue of Function on Malignant Calmodulinopathic Long-QT Syndrome.

PubMed

Limpitikul, Worawan B; Dick, Ivy E; Tester, David J; Boczek, Nicole J; Limphong, Pattraranee; Yang, Wanjun; Choi, Myoung Hyun; Babich, Jennifer; DiSilvestre, Deborah; Kanter, Ronald J; Tomaselli, Gordon F; Ackerman, Michael J; Yue, David T

2017-01-06

Calmodulinopathies comprise a new category of potentially life-threatening genetic arrhythmia syndromes capable of producing severe long-QT syndrome (LQTS) with mutations involving CALM1, CALM2, or CALM3. The underlying basis of this form of LQTS is a disruption of Ca 2+ /calmodulin (CaM)-dependent inactivation of L-type Ca 2+ channels. To gain insight into the mechanistic underpinnings of calmodulinopathies and devise new therapeutic strategies for the treatment of this form of LQTS. We generated and characterized the functional properties of induced pluripotent stem cell-derived cardiomyocytes from a patient with D130G-CALM2-mediated LQTS, thus creating a platform with which to devise and test novel therapeutic strategies. The patient-derived induced pluripotent stem cell-derived cardiomyocytes display (1) significantly prolonged action potentials, (2) disrupted Ca 2+ cycling properties, and (3) diminished Ca 2+ /CaM-dependent inactivation of L-type Ca 2+ channels. Next, taking advantage of the fact that calmodulinopathy patients harbor a mutation in only 1 of 6 redundant CaM-encoding alleles, we devised a strategy using CRISPR interference to selectively suppress the mutant gene while sparing the wild-type counterparts. Indeed, suppression of CALM2 expression produced a functional rescue in induced pluripotent stem cell-derived cardiomyocytes with D130G-CALM2, as shown by the normalization of action potential duration and Ca 2+ /CaM-dependent inactivation after treatment. Moreover, CRISPR interference can be designed to achieve selective knockdown of any of the 3 CALM genes, making it a generalizable therapeutic strategy for any calmodulinopathy. Overall, this therapeutic strategy holds great promise for calmodulinopathy patients as it represents a generalizable intervention capable of specifically altering CaM expression and potentially attenuating LQTS-triggered cardiac events, thus initiating a path toward precision medicine. © 2016 American Heart Association, Inc.

[Assessment of precision and accuracy of digital surface photogrammetry with the DSP 400 system].

PubMed

Krimmel, M; Kluba, S; Dietz, K; Reinert, S

2005-03-01

The objective of the present study was to evaluate the precision and accuracy of facial anthropometric measurements obtained through digital 3-D surface photogrammetry with the DSP 400 system in comparison to traditional 2-D photogrammetry. Fifty plaster casts of cleft infants were imaged and 21 standard anthropometric measurements were obtained. For precision assessment the measurements were performed twice in a subsample. Accuracy was determined by comparison of direct measurements and indirect 2-D and 3-D image measurements. Precision of digital surface photogrammetry was almost as good as direct anthropometry and clearly better than 2-D photogrammetry. Measurements derived from 3-D images showed better congruence to direct measurements than from 2-D photos. Digital surface photogrammetry with the DSP 400 system is sufficiently precise and accurate for craniofacial anthropometric examinations.

Therapeutic Decision-Making in Endoscopically Unmanageable Nonvariceal Upper Gastrointestinal Hemorrhage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Defreyne, Luc, E-mail: Luc.Defreyne@UGent.b; Schrijver, Ignace De; Decruyenaere, Johan

2008-09-15

The purpose of this study was to identify endoscopic and clinical parameters influencing the decision-making in salvage of endoscopically unmanageable, nonvariceal upper gastrointestinal hemorrhage (UGIH) and to report the outcome of selected therapy. We retrospectively retrieved all cases of surgery and arteriography for arrest of endoscopically unmanageable UGIH. Only patients with overt bleeding on endoscopy within the previous 24 h were included. Patients with preceding nonendoscopic hemostatic interventions, portal hypertension, malignancy, and transpapillar bleeding were excluded. Potential clinical and endoscopic predictors of allocation to either surgery or arteriography were tested using statistical models. Outcome and survival were regressed on themore » choice of rescue and clinical variables. Forty-six arteriographed and 51 operated patients met the inclusion criteria. Univariate analysis revealed a higher number of patients with a coagulation disorder in the catheterization group (41.4%, versus 20.4% in the laparotomy group; p = 0.044). With multivariate analysis, the identification of a bleeding peptic ulcer at endoscopy significantly steered decision-making toward surgical rescue (OR = 5.2; p = 0.021). Taking into account reinterventions, hemostasis was achieved in nearly 90% of cases in both groups. Overall therapy failure (no survivors), rebleeding within 3 days (OR = 3.7; p = 0.042), and corticosteroid use (OR = 5.2; p = 0.017) had a significant negative impact on survival. The odds of dying were not different for embolotherapy or surgery. In conclusion, decision-making was endoscopy-based, with bleeding peptic ulcer significantly directing the choice of rescue toward surgery. Unsuccessful hemostasis and corticosteroid use, but not the choice of rescue, negatively affected outcome.« less

Targeted rescue of a polycystic kidney disease mutation by lysosomal inhibition.

PubMed

Hofherr, Alexis; Wagner, Claudius J; Watnick, Terry; Köttgen, Michael

2016-04-01

Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenic cause of end-stage renal disease. The molecular pathogenesis of ADPKD is not completely known, and there is no approved therapy. To date, there is limited knowledge concerning the molecular consequences of specific disease-causing mutations. Here we show that the ADPKD missense variant TRPP2(D511V) greatly reduces TRPP2 protein stability, and that TRPP2(D511V) function can be rescued in vivo by small molecules targeting the TRPP2 degradation pathway. Expression of the TRPP2(D511V) protein was significantly reduced compared to wild-type TRPP2. Inhibition of lysosomal degradation of TRPP2(D511V) by the US Food and Drug Administration (FDA)-approved drug chloroquine strongly increased TRPP2 protein levels in vitro. The validation of these results in vivo requires appropriate animal models. However, there are currently no mouse models harboring human PKD2 missense mutations, and screening for chemical rescue of patient mutations in rodent models is time-consuming and expensive. Therefore, we developed a Drosophila melanogaster model expressing the ortholog of TRPP2(D511V) to test chemical rescue of mutant TRPP2 in vivo. Notably, chloroquine was sufficient to improve the phenotype of flies expressing mutant TRPP2. Thus, this proof-of-concept study highlights the potential of directed therapeutic approaches for ADPKD, and provides a rapid-throughput experimental model to screen PKD2 patient mutations and small molecules in vivo. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Precision gap particle separator

DOEpatents

Benett, William J.; Miles, Robin; Jones, II., Leslie M.; Stockton, Cheryl

2004-06-08

A system for separating particles entrained in a fluid includes a base with a first channel and a second channel. A precision gap connects the first channel and the second channel. The precision gap is of a size that allows small particles to pass from the first channel into the second channel and prevents large particles from the first channel into the second channel. A cover is positioned over the base unit, the first channel, the precision gap, and the second channel. An port directs the fluid containing the entrained particles into the first channel. An output port directs the large particles out of the first channel. A port connected to the second channel directs the small particles out of the second channel.

30 CFR Appendix to Subpart B of... - Optional Form for Certifying Mine Rescue Teams

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Optional Form for Certifying Mine Rescue Teams... LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines Pt. 49, Subpt. B, App. Appendix to Subpart B of Part 49—Optional Form for Certifying Mine Rescue Teams ER08FE08...

30 CFR Appendix to Subpart B of... - Optional Form for Certifying Mine Rescue Teams

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Optional Form for Certifying Mine Rescue Teams... LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines Pt. 49, Subpt. B, App. Appendix to Subpart B of Part 49—Optional Form for Certifying Mine Rescue Teams ER08FE08...

30 CFR Appendix to Subpart B - Optional Form for Certifying Mine Rescue Teams

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Optional Form for Certifying Mine Rescue Teams... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines Pt. 49, Subpt. B, App. Appendix to Subpart B—Optional Form for Certifying Mine Rescue Teams ER08FE08.000 ER08FE08.001 ...

30 CFR Appendix to Subpart B - Optional Form for Certifying Mine Rescue Teams

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Optional Form for Certifying Mine Rescue Teams... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines Pt. 49, Subpt. B, App. Appendix to Subpart B—Optional Form for Certifying Mine Rescue Teams ER08FE08.000 ER08FE08.001 ...

30 CFR Appendix to Subpart B - Optional Form for Certifying Mine Rescue Teams

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Optional Form for Certifying Mine Rescue Teams... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines Pt. 49, Subpt. B, App. Appendix to Subpart B—Optional Form for Certifying Mine Rescue Teams ER08FE08.000 ER08FE08.001 ...

Failure-to-rescue after injury is associated with preventability: The results of mortality panel review of failure-to-rescue cases in trauma

PubMed Central

Kuo, Lindsay E.; Kaufman, Elinore; Hoffman, Rebecca L.; Pascual, Jose L.; Martin, Niels D.; Kelz, Rachel R.; Holena, Daniel N.

2018-01-01

Background Failure-to-rescue is defined as the conditional probability of death after a complication, and the failure-to-rescue rate reflects a center’s ability to successfully “rescue” patients after complications. The validity of the failure-to-rescue rate as a quality measure is dependent on the preventability of death and the appropriateness of this measure for use in the trauma population is untested. We sought to evaluate the relationship between preventability and failure-to-rescue in trauma. Methods All adjudications from a mortality review panel at an academic level I trauma center from 2005–2015 were merged with registry data for the same time period. The preventability of each death was determined by panel consensus as part of peer review. Failure-to-rescue deaths were defined as those occurring after any registry-defined complication. Univariate and multivariate logistic regression models between failure-to-rescue status and preventability were constructed and time to death was examined using survival time analyses. Results Of 26,557 patients, 2,735 (10.5%) had a complication, of whom 359 died for a failure-to-rescue rate of 13.2%. Of failure-to-rescue deaths, 272 (75.6%) were judged to be non-preventable, 65 (18.1%) were judged potentially preventable, and 22 (6.1%) were judged to be preventable by peer review. After adjusting for other patient factors, there remained a strong association between failure-to-rescue status and potentially preventable (odds ratio 2.32, 95% confidence interval, 1.47–3.66) and preventable (odds ratio 14.84, 95% confidence interval, 3.30–66.71) judgment. Conclusion Despite a strong association between failure-to-rescue status and preventability adjudication, only a minority of deaths meeting the definition of failure to rescue were judged to be preventable or potentially preventable. Revision of the failure-to-rescue metric before use in trauma care benchmarking is warranted. PMID:27788924

Regulation of Msx genes by a Bmp gradient is essential for neural crest specification.

PubMed

Tribulo, Celeste; Aybar, Manuel J; Nguyen, Vu H; Mullins, Mary C; Mayor, Roberto

2003-12-01

There is evidence in Xenopus and zebrafish embryos that the neural crest/neural folds are specified at the border of the neural plate by a precise threshold concentration of a Bmp gradient. In order to understand the molecular mechanism by which a gradient of Bmp is able to specify the neural crest, we analyzed how the expression of Bmp targets, the Msx genes, is regulated and the role that Msx genes has in neural crest specification. As Msx genes are directly downstream of Bmp, we analyzed Msx gene expression after experimental modification in the level of Bmp activity by grafting a bead soaked with noggin into Xenopus embryos, by expressing in the ectoderm a dominant-negative Bmp4 or Bmp receptor in Xenopus and zebrafish embryos, and also through Bmp pathway component mutants in the zebrafish. All the results show that a reduction in the level of Bmp activity leads to an increase in the expression of Msx genes in the neural plate border. Interestingly, by reaching different levels of Bmp activity in animal cap ectoderm, we show that a specific concentration of Bmp induces msx1 expression to a level similar to that required to induce neural crest. Our results indicate that an intermediate level of Bmp activity specifies the expression of Msx genes in the neural fold region. In addition, we have analyzed the role that msx1 plays on neural crest specification. As msx1 has a role in dorsoventral pattering, we have carried out conditional gain- and loss-of-function experiments using different msx1 constructs fused to a glucocorticoid receptor element to avoid an early effect of this factor. We show that msx1 expression is able to induce all other early neural crest markers tested (snail, slug, foxd3) at the time of neural crest specification. Furthermore, the expression of a dominant negative of Msx genes leads to the inhibition of all the neural crest markers analyzed. It has been previously shown that snail is one of the earliest genes acting in the neural crest genetic cascade. In order to study the hierarchical relationship between msx1 and snail/slug we performed several rescue experiments using dominant negatives for these genes. The rescuing activity by snail and slug on neural crest development of the msx1 dominant negative, together with the inability of msx1 to rescue the dominant negatives of slug and snail strongly argue that msx1 is upstream of snail and slug in the genetic cascade that specifies the neural crest in the ectoderm. We propose a model where a gradient of Bmp activity specifies the expression of Msx genes in the neural folds, and that this expression is essential for the early specification of the neural crest.

[Design and application of portable rescue vehicle].

PubMed

Guo, Ying; Qi, Huaying; Wang, Shen

2017-12-01

The disease of critically ill patients was with rapid changes, and at any time faced the risk of emergency. The current commonly used rescue vehicles were larger and bulky implementation, which were not conducive to the operation, therefore the design of a portable rescue vehicle was needed. This new type of rescue vehicle is multi-layer folding structure, with small footprint, large storage space, so a variety of first aid things can be classified and put, easy to be cleaned and disinfected. In the rescue process, the portable rescue vehicles can be placed in the required position; box of various emergency items can be found at a glance with easy access; the height of the infusion stand can adjust freely according to the user height; the rescue vehicle handle can be easy to pull and adjust accord with human body mechanics principle. The portable rescue vehicle facilitates the operation of medical staff, and is worthy of clinical application.

Bench evaluation: three face-shield CPR barrier devices.

PubMed

Simmons, M; Deao, D; Moon, L; Peters, K; Cavanaugh, S

1995-06-01

Due to the fear of disease transmission, the practice of mouth-to-mouth (M-M) rescue breathing is rarely performed; to address this concern, many types of CPR barrier devices have been developed. These include bag-valve-mask devices, mouth-to-mask devices, and face shields (FS). The purpose of this study was to measure the volumes delivered during mouth-to-face shield (M-FS) breathing, to measure the back pressure and calculate the resistance to flow through their 1-way valves, and to test for backward leak of gas through the valves. Three FS brands were evaluated: Kiss of Life (KOL), MicroSHIELD (Micro) and Res-Cue Key (RCK). Volume delivered during M-M and M-FS breathing was evaluated by 10 rescuers who used the devices while performing rescue breathing on a CPR mannequin. Back pressure was measured and resistance calculated by directing airflow through the 1-way valves. Backward leak was evaluated by measuring the O2 concentration at the rescuer side of the valve while 100% O2 was directed toward the patient side of the valve. Differences among the brands were evaluated using analysis of variance. The mean (SD) values for volumes in L were: M-M 1.00 (0.25), Micro 0.77 (0.20), RCK 0.64 (0.10), and KOL 0.24 (0.11). Mean values for back pressure in cm H2O at 50 L/min were Micro 16.7 (1.29), KOL 7.22 (0.13), and RCK 2.15 (0.16). Significant backward leak only occurred with RCK. Not one of the FSs tested met all of the requirements suggested by the American Heart Association and by the International Standards Organization.

MiR-124 down-regulation is critical for cancer associated fibroblasts-enhanced tumor growth of oral carcinoma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Xia, E-mail: dentistlx@163.com; Fan, Qinqiao; Li, Jinyun

Cancer associated fibroblasts (CAFs) are known to be involved in initiation, progression and metastasis of various cancers. However, the molecular mechanism of how CAFs affects the biological function of oral cancer (OC) has not been fully-addressed. In this study, we demonstrated that miR-124 was downregulated in oral CAFs and oral cancer cells (OCCs) when compared with matched normal fibroblasts (NFs). Hypermethylation in the promoter region of miR-124 genes was accounted for its downregulation. Interestingly, CAFs but not NFs exerted promotion effect on OCCs cell proliferation, migration and tumor growth in CAFs/NFs-OCCs co-culture. Furthermore, we identified Chemokine (C-C motif) ligand 2more » (CCL2) and Interleukin 8 (IL-8) as two direct targets of miR-124. Over-expression of miR-124 in CAFs-OCCs co-culture abrogated CAFs-promoted OCCs cell growth and migration, and this inhibitory effect can be rescued by addition of CCL2 and IL-8. Finally, we showed that restoration of miR-124 expression by lentiviral infection or formulated miR-124 injection inhibited oral tumor growth in vivo suggesting miR-124 rescue could be a potential rationale for therapeutic applications in oral cancer in the future. - Highlights: • miR-124 was downregulated in oral cancer cells and cancer associated fibroblasts. • Hypermethylation in the promoter region was accounted for miR-124 downregulation. • CCL2 and IL-8 are two direct targets of miR-124. • miR-124 rescue could be a potential rationale for oral cancer therapy.« less

Differential sensitivities of cellular XPA and PARP-1 to arsenite inhibition and zinc rescue.

PubMed

Ding, Xiaofeng; Zhou, Xixi; Cooper, Karen L; Huestis, Juliana; Hudson, Laurie G; Liu, Ke Jian

2017-09-15

Arsenite directly binds to the zinc finger domains of the DNA repair protein poly (ADP ribose) polymerase (PARP)-1, and inhibits PARP-1 activity in the base excision repair (BER) pathway. PARP inhibition by arsenite enhances ultraviolet radiation (UVR)-induced DNA damage in keratinocytes, and the increase in DNA damage is reduced by zinc supplementation. However, little is known about the effects of arsenite and zinc on the zinc finger nucleotide excision repair (NER) protein xeroderma pigmentosum group A (XPA). In this study, we investigated the difference in response to arsenite exposure between XPA and PARP-1, and the differential effectiveness of zinc supplementation in restoring protein DNA binding and DNA damage repair. Arsenite targeted both XPA and PARP-1 in human keratinocytes, resulting in zinc loss from each protein and a pronounced decrease in XPA and PARP-1 binding to chromatin as demonstrated by Chip-on-Western assays. Zinc effectively restored DNA binding of PARP-1 and XPA to chromatin when zinc concentrations were equal to those of arsenite. In contrast, zinc was more effective in rescuing arsenite-augmented direct UVR-induced DNA damage than oxidative DNA damage. Taken together, our findings indicate that arsenite interferes with PARP-1 and XPA binding to chromatin, and that zinc supplementation fully restores DNA binding activity to both proteins in the cellular context. Interestingly, rescue of arsenite-inhibited DNA damage repair by supplemental zinc was more sensitive for DNA damage repaired by the XPA-associated NER pathway than for the PARP-1-dependent BER pathway. This study expands our understanding of arsenite's role in DNA repair inhibition and co-carcinogenesis. Copyright © 2017 Elsevier Inc. All rights reserved.

Probing Conformational Rescue Induced by a Chemical Corrector of F508del-Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Mutant*

PubMed Central

Yu, Wilson; Chiaw, Patrick Kim; Bear, Christine E.

2011-01-01

Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene that cause loss of function of the CFTR channel on the apical surface of epithelial cells. The major CF-causing mutation, F508del-CFTR, is misfolded, retained in the endoplasmic reticulum, and degraded. Small molecule corrector compounds have been identified using high throughput screens, which partially rescue the trafficking defect of F508del-CFTR, allowing a fraction of the mutant protein to escape endoplasmic reticulum retention and traffic to the plasma membrane, where it exhibits partial function as a cAMP-regulated chloride channel. A subset of such corrector compounds binds directly to the mutant protein, prompting the hypothesis that they rescue the biosynthetic defect by inducing improved protein conformation. We tested this hypothesis directly by evaluating the consequences of a corrector compound on the conformation of each nucleotide binding domain (NBD) in the context of the full-length mutant protein in limited proteolytic digest studies. Interestingly, we found that VRT-325 was capable of partially restoring compactness in NBD1. However, VRT-325 had no detectable effect on the conformation of the second half of the molecule. In comparison, ablation of the di-arginine sequence, R553XR555 (F508del-KXK-CFTR), modified protease susceptibility of NBD1, NBD2, and the full-length protein. Singly, each intervention led to a partial correction of the processing defect. Together, these interventions restored processing of F508del-CFTR to near wild type. Importantly, however, a defect in NBD1 conformation persisted, as did a defect in channel activation after the combined interventions. Importantly, this defect in channel activation can be fully corrected by the addition of the potentiator, VX-770. PMID:21602569

30 CFR 49.3 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and... miners willing to serve on a mine rescue team; (8) The operator's alternative plan for assuring that a...

30 CFR 49.3 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and... miners willing to serve on a mine rescue team; (8) The operator's alternative plan for assuring that a...

30 CFR 49.3 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and... miners willing to serve on a mine rescue team; (8) The operator's alternative plan for assuring that a...

30 CFR 49.13 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines... the operator as to the number of miners willing to serve on a mine rescue team; (8) The operator's...

30 CFR 49.13 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines... the operator as to the number of miners willing to serve on a mine rescue team; (8) The operator's...

30 CFR 49.13 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines... the operator as to the number of miners willing to serve on a mine rescue team; (8) The operator's...

30 CFR 49.13 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines... the operator as to the number of miners willing to serve on a mine rescue team; (8) The operator's...

30 CFR 49.13 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines... the operator as to the number of miners willing to serve on a mine rescue team; (8) The operator's...

The Mysterious Rescue of adg1-1/tpt-2 – an Arabidopsis thaliana Double Mutant Impaired in Acclimation to High Light – by Exogenously Supplied Sugars

PubMed Central

Heinrichs, Luisa; Schmitz, Jessica; Flügge, Ulf-Ingo; Häusler, Rainer E.

2012-01-01

An Arabidopsis thaliana double mutant (adg1-1/tpt-2) defective in the day- and night-path of photoassimilate export from the chloroplast due to a knockout in the triose phosphate/phosphate translocator (TPT; tpt-2) and a lack of starch [mutation in ADP glucose pyrophosphorylase (AGPase); adg1-1] exhibits severe growth retardation, a decrease in the photosynthetic capacity, and a high chlorophyll fluorescence (HCF) phenotype under high light conditions. These phenotypes could be rescued when the plants were grown on sucrose (Suc) or glucose (Glc). Here we address the question whether Glc-sensing hexokinase1 (HXK1) defective in the Glc insensitive 2 (gin2-1) mutant is involved in the sugar-dependent rescue of adg1-1/tpt-2. Triple mutants defective in the TPT, AGPase, and HXK1 (adg1-1/tpt-2/gin2-1) were established as homozygous lines and grown together with Col-0 and Landsberg erecta (Ler) wild-type plants, gin2-1, the adg1-1/tpt-2 double mutant, and the adg1-1/tpt-2/gpt2-1 triple mutant [additionally defective in the glucose 6-phosphate/phosphate translocator 2 (GPT2)] on agar in the presence or absence of 50 mM of each Glc, Suc, or fructose (Fru). The growth phenotype of the double mutant and both triple mutants could be rescued to a similar extent only by Glc and Suc, but not by Fru. All three sugars were capable of rescuing the HCF and photosynthesis phenotype, irrespectively of the presence or absence of HXK1. Quantitative RT-PCR analyses of sugar-responsive genes revealed that plastidial HXK (pHXK) was up-regulated in adg1-1/tpt-2 plants grown on sugars, but showed no response in adg1-1/tpt-2/gin2-1. It appears likely that soluble sugars are directly taken up by the chloroplasts and enter further metabolism, which consumes ATP and NADPH from the photosynthetic light reaction and thereby rescues the photosynthesis phenotype of the double mutant. The implication of sugar turnover and probably signaling inside the chloroplasts for the concept of retrograde signaling is discussed. PMID:23233856

African Swine Fever Virus NP868R Capping Enzyme Promotes Reovirus Rescue during Reverse Genetics by Promoting Reovirus Protein Expression, Virion Assembly, and RNA Incorporation into Infectious Virions

PubMed Central

Eaton, Heather E.; Kobayashi, Takeshi; Dermody, Terence S.; Johnston, Randal N.

2017-01-01

ABSTRACT Reoviruses, like many eukaryotic viruses, contain an inverted 7-methylguanosine (m7G) cap linked to the 5′ nucleotide of mRNA. The traditional functions of capping are to promote mRNA stability, protein translation, and concealment from cellular proteins that recognize foreign RNA. To address the role of mRNA capping during reovirus replication, we assessed the benefits of adding the African swine fever virus NP868R capping enzyme during reovirus rescue. C3P3, a fusion protein containing T7 RNA polymerase and NP868R, was found to increase protein expression 5- to 10-fold compared to T7 RNA polymerase alone while enhancing reovirus rescue from the current reverse genetics system by 100-fold. Surprisingly, RNA stability was not increased by C3P3, suggesting a direct effect on protein translation. A time course analysis revealed that C3P3 increased protein synthesis within the first 2 days of a reverse genetics transfection. This analysis also revealed that C3P3 enhanced processing of outer capsid μ1 protein to μ1C, a previously described hallmark of reovirus assembly. Finally, to determine the rate of infectious-RNA incorporation into new virions, we developed a new recombinant reovirus S1 gene that expressed the fluorescent protein UnaG. Following transfection of cells with UnaG and infection with wild-type virus, passage of UnaG through progeny was significantly enhanced by C3P3. These data suggest that capping provides nontraditional functions to reovirus, such as promoting assembly and infectious-RNA incorporation. IMPORTANCE Our findings expand our understanding of how viruses utilize capping, suggesting that capping provides nontraditional functions to reovirus, such as promoting assembly and infectious-RNA incorporation, in addition to enhancing protein translation. Beyond providing mechanistic insight into reovirus replication, our findings also show that reovirus reverse genetics rescue is enhanced 100-fold by the NP868R capping enzyme. Since reovirus shows promise as a cancer therapy, efficient reovirus reverse genetics rescue will accelerate production of recombinant reoviruses as candidates to enhance therapeutic potency. NP868R-assisted reovirus rescue will also expedite production of recombinant reovirus for mechanistic insights into reovirus protein function and structure. PMID:28298603

Pathophysiology of luteal-phase deficiency in human reproduction.

PubMed

Nakajima, S T; Gibson, M

1991-03-01

There are numerous probable mechanisms for the clinical occurrence of a luteal-phase deficiency. Defects may occur in either the proliferative, luteal, or luteal-rescue stage of a menstrual cycle. In each of these three domains, alterations in the trophic stimulation or the response at either the ovarian or endometrial level further subdivide the etiologies for luteal-phase deficiency. Additional development of new concepts in the areas of intraovarian signaling, the possible role of growth factors, and the measurement of newly discovered luteal products will enable us to expand our thought process. With a better understanding of the pathophysiology of luteal-phase deficiency, it is anticipated that new treatments will be devised to address precisely a given specific etiologic factor.

Kinematic Simulation of a universal rescue vehicle

NASA Astrophysics Data System (ADS)

Vasiliev, I. A.; Smirnova, E. Y.; Krasheninnikov, M. S.; Koshurina, A. A.; Dorofeev, R. A.; E Gai, V.

2017-01-01

The rescue of people in disaster through autonomous means of evacuation in some cases is the only way to save their lives. Rescue Mission often takes place in remote locations. The paper studies modeling of a universal rescue vehicle with a rotary-screw propeller.

30 CFR 49.3 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.3 Alternative mine rescue capability for... statement by the operator as to the number of miners willing to serve on a mine rescue team; (8) The...

30 CFR 49.3 - Alternative mine rescue capability for small and remote mines.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.3 Alternative mine rescue capability for... statement by the operator as to the number of miners willing to serve on a mine rescue team; (8) The...

The deubiquitinating enzyme DUBAI stabilizes DIAP1 to suppress Drosophila apoptosis

PubMed Central

Yang, C-S; Sinenko, S A; Thomenius, M J; Robeson, A C; Freel, C D; Horn, S R; Kornbluth, S

2014-01-01

Deubiquitinating enzymes (DUBs) counteract ubiquitin ligases to modulate the ubiquitination and stability of target signaling molecules. In Drosophila, the ubiquitin–proteasome system has a key role in the regulation of apoptosis, most notably, by controlling the abundance of the central apoptotic regulator DIAP1. Although the mechanism underlying DIAP1 ubiquitination has been extensively studied, the precise role of DUB(s) in controlling DIAP1 activity has not been fully investigated. Here we report the identification of a DIAP1-directed DUB using two complementary approaches. First, a panel of putative Drosophila DUBs was expressed in S2 cells to determine whether DIAP1 could be stabilized, despite treatment with death-inducing stimuli that would induce DIAP1 degradation. In addition, RNAi fly lines were used to detect modifiers of DIAP1 antagonist-induced cell death in the developing eye. Together, these approaches identified a previously uncharacterized protein encoded by CG8830, which we named DeUBiquitinating-Apoptotic-Inhibitor (DUBAI), as a novel DUB capable of preserving DIAP1 to dampen Drosophila apoptosis. DUBAI interacts with DIAP1 in S2 cells, and the putative active site of its DUB domain (C367) is required to rescue DIAP1 levels following apoptotic stimuli. DUBAI, therefore, represents a novel locus of apoptotic regulation in Drosophila, antagonizing cell death signals that would otherwise result in DIAP1 degradation. PMID:24362437

In situ genetic correction of F8 intron 22 inversion in hemophilia A patient-specific iPSCs.

PubMed

Wu, Yong; Hu, Zhiqing; Li, Zhuo; Pang, Jialun; Feng, Mai; Hu, Xuyun; Wang, Xiaolin; Lin-Peng, Siyuan; Liu, Bo; Chen, Fangping; Wu, Lingqian; Liang, Desheng

2016-01-08

Nearly half of severe Hemophilia A (HA) cases are caused by F8 intron 22 inversion (Inv22). This 0.6-Mb inversion splits the 186-kb F8 into two parts with opposite transcription directions. The inverted 5' part (141 kb) preserves the first 22 exons that are driven by the intrinsic F8 promoter, leading to a truncated F8 transcript due to the lack of the last 627 bp coding sequence of exons 23-26. Here we describe an in situ genetic correction of Inv22 in patient-specific induced pluripotent stem cells (iPSCs). By using TALENs, the 627 bp sequence plus a polyA signal was precisely targeted at the junction of exon 22 and intron 22 via homologous recombination (HR) with high targeting efficiencies of 62.5% and 52.9%. The gene-corrected iPSCs retained a normal karyotype following removal of drug selection cassette using a Cre-LoxP system. Importantly, both F8 transcription and FVIII secretion were rescued in the candidate cell types for HA gene therapy including endothelial cells (ECs) and mesenchymal stem cells (MSCs) derived from the gene-corrected iPSCs. This is the first report of an efficient in situ genetic correction of the large inversion mutation using a strategy of targeted gene addition.

In situ genetic correction of F8 intron 22 inversion in hemophilia A patient-specific iPSCs

PubMed Central

Wu, Yong; Hu, Zhiqing; Li, Zhuo; Pang, Jialun; Feng, Mai; Hu, Xuyun; Wang, Xiaolin; Lin-Peng, Siyuan; Liu, Bo; Chen, Fangping; Wu, Lingqian; Liang, Desheng

2016-01-01

Nearly half of severe Hemophilia A (HA) cases are caused by F8 intron 22 inversion (Inv22). This 0.6-Mb inversion splits the 186-kb F8 into two parts with opposite transcription directions. The inverted 5′ part (141 kb) preserves the first 22 exons that are driven by the intrinsic F8 promoter, leading to a truncated F8 transcript due to the lack of the last 627 bp coding sequence of exons 23–26. Here we describe an in situ genetic correction of Inv22 in patient-specific induced pluripotent stem cells (iPSCs). By using TALENs, the 627 bp sequence plus a polyA signal was precisely targeted at the junction of exon 22 and intron 22 via homologous recombination (HR) with high targeting efficiencies of 62.5% and 52.9%. The gene-corrected iPSCs retained a normal karyotype following removal of drug selection cassette using a Cre-LoxP system. Importantly, both F8 transcription and FVIII secretion were rescued in the candidate cell types for HA gene therapy including endothelial cells (ECs) and mesenchymal stem cells (MSCs) derived from the gene-corrected iPSCs. This is the first report of an efficient in situ genetic correction of the large inversion mutation using a strategy of targeted gene addition. PMID:26743572

[Medical Emergency Preparedness in offshore wind farms : New challenges in the german north and baltic seas].

PubMed

Stuhr, M; Dethleff, D; Weinrich, N; Nielsen, M; Hory, D; Kowald, B; Seide, K; Kerner, T; Nau, C; Jürgens, C

2016-05-01

Offshore windfarms are constructed in the German North and Baltic Seas. The off-coast remoteness of the windfarms, particular environmental conditions, limitations in offshore structure access, working in heights and depths, and the vast extent of the offshore windfarms cause significant challenges for offshore rescue. Emergency response systems comparable to onshore procedures are not fully established yet. Further, rescue from offshore windfarms is not part of the duty of the German Maritime Search and Rescue Organization or SAR-Services due to statute and mandate reasons. Scientific recommendations or guidelines for rescue from offshore windfarms are not available yet. The present article reflects the current state of medical care and rescue from German offshore windfarms and related questions. The extended therapy-free interval until arrival of the rescue helicopter requires advanced first-aid measures as well as improved first-aider qualification. Rescue helicopters need to be equipped with a winch system in order to dispose rescue personnel on the wind turbines, and to hoist-up patients. For redundancy reasons and for conducting rendezvous procedures, adequate sea-bound rescue units need to be provided. In the light of experiences from the offshore oil and gas industry and first offshore wind analyses, the availability of professional medical personnel in offshore windfarms seems advisible. Operational air medical rescue services and specific offshore emergency reaction teams have established a powerful rescue chain. Besides the present development of medical standards, more studies are necessary in order to place the rescue chain on a long-term, evidence-based groundwork. A central medical offshore registry may help to make a significant contribution at this point.

Exploring the Interplay between Rescue Drugs, Data Imputation, and Study Outcomes: Conceptual Review and Qualitative Analysis of an Acute Pain Data Set.

PubMed

Singla, Neil K; Meske, Diana S; Desjardins, Paul J

2017-12-01

In placebo-controlled acute surgical pain studies, provisions must be made for study subjects to receive adequate analgesic therapy. As such, most protocols allow study subjects to receive a pre-specified regimen of open-label analgesic drugs (rescue drugs) as needed. The selection of an appropriate rescue regimen is a critical experimental design choice. We hypothesized that a rescue regimen that is too liberal could lead to all study arms receiving similar levels of pain relief (thereby confounding experimental results), while a regimen that is too stringent could lead to a high subject dropout rate (giving rise to a preponderance of missing data). Despite the importance of rescue regimen as a study design feature, there exist no published review articles or meta-analysis focusing on the impact of rescue therapy on experimental outcomes. Therefore, when selecting a rescue regimen, researchers must rely on clinical factors (what analgesics do patients usually receive in similar surgical scenarios) and/or anecdotal evidence. In the following article, we attempt to bridge this gap by reviewing and discussing the experimental impacts of rescue therapy on a common acute surgical pain population: first metatarsal bunionectomy. The function of this analysis is to (1) create a framework for discussion and future exploration of rescue as a methodological study design feature, (2) discuss the interplay between data imputation techniques and rescue drugs, and (3) inform the readership regarding the impact of data imputation techniques on the validity of study conclusions. Our findings indicate that liberal rescue may degrade assay sensitivity, while stringent rescue may lead to unacceptably high dropout rates.

Tolvaptan rescue contrast-induced acute kidney injury: A case report.

PubMed

Lee, Wei-Chieh; Fang, Hsiu-Yu; Fang, Chih-Yuan

2018-04-01

Contrast-induced acute kidney injury is one of the most serious adverse effects of contrast media and is related to three distinct but interacting mechanisms: medullary ischemia, formation of reactive oxygen species and direct tubular cell toxicity, especially in the patients with chronic kidney disease. The strategies of treatment, including stabilization of hemodynamic parameters and maintenance of normal fluid and electrolyte balance, were similar to the management of other types of acute kidney injury. A 58-year-old woman experienced acute oligouria after complex percutaneous coronary intervention for multiple vessel coronary artery disease. Chest radiography showed pulmonary congestion and hyponatremia was noted after fluid hydration for suspicious contrast-induced nephropathy. Oral tolvaptan, at 15mg per day, was used for three days. Urine output increased gradually and symptoms relieved one day later after using tolvaptan. Serum creatinine also improved to baseline level one week later after this event. Here, we reported an interesting case about contrast-induced acute kidney injury and hypervolemic hyponatremia, where tolvaptan was used to rescue the oliguric phase. Tolvaptan could be considered to use for contrast-induced acute kidney injury and had possibility of prevention from hemodialysis. Larger studies are still needed to investigate the role of tolvaptan in rescuing the oliguric phase in contrast-induced acute kidney injury.

77 FR 39745 - General Aviation Search and Rescue

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

... NATIONAL TRANSPORTATION SAFETY BOARD General Aviation Search and Rescue The National Transportation Safety Board (NTSB) will convene a 2- day forum focused on general aviation search and rescue..., inland searches for the aircraft are conducted by the Air Force Rescue Coordination Center, who are...

46 CFR 11.321 - Requirements to qualify for an STCW endorsement as officer in charge of a navigational watch...

Code of Federal Regulations, 2014 CFR

2014-10-01

... subpart. (iii) Proficiency in survival craft and rescue boats other than fast rescue boats (PSC) or proficiency in survival craft and rescue boats other than lifeboats and fast rescue boats (PSC-limited). (iv...

Issues in intelligent robots for search and rescue

NASA Astrophysics Data System (ADS)

Casper, Jennifer L.; Micire, Mark; Murphy, Robin R.

2000-07-01

Since the 1995 Oklahoma City bombing and Kobe, Japan, earthquake, robotics researchers have been considering search and rescue as a humanitarian research domain. The recent devastation in Turkey and Taiwan, compounded with the new Robocup Rescue and AAAI Urban Search and Rescue robot competition, may encourage more research. However, roboticists generally go not have access to domain experts: the emergency workers or first providers. This paper shares our understanding of urban search and rescue, based on our active research in this area and training sessions with rescue workers from the Hillsborough County (Florida) Fire Departments. The paper is intended to be a stepping stone for roboticists entering the field.

Menin: A Tumor Suppressor That Mediates Postsynaptic Receptor Expression and Synaptogenesis between Central Neurons of Lymnaea stagnalis

PubMed Central

Flynn, Nichole; Getz, Angela; Visser, Frank; Janes, Tara A.; Syed, Naweed I.

2014-01-01

Neurotrophic factors (NTFs) support neuronal survival, differentiation, and even synaptic plasticity both during development and throughout the life of an organism. However, their precise roles in central synapse formation remain unknown. Previously, we demonstrated that excitatory synapse formation in Lymnaea stagnalis requires a source of extrinsic NTFs and receptor tyrosine kinase (RTK) activation. Here we show that NTFs such as Lymnaea epidermal growth factor (L-EGF) act through RTKs to trigger a specific subset of intracellular signalling events in the postsynaptic neuron, which lead to the activation of the tumor suppressor menin, encoded by Lymnaea MEN1 (L-MEN1) and the expression of excitatory nicotinic acetylcholine receptors (nAChRs). We provide direct evidence that the activation of the MAPK/ERK cascade is required for the expression of nAChRs, and subsequent synapse formation between pairs of neurons in vitro. Furthermore, we show that L-menin activation is sufficient for the expression of postsynaptic excitatory nAChRs and subsequent synapse formation in media devoid of NTFs. By extending our findings in situ, we reveal the necessity of EGFRs in mediating synapse formation between a single transplanted neuron and its intact presynaptic partner. Moreover, deficits in excitatory synapse formation following EGFR knock-down can be rescued by injecting synthetic L-MEN1 mRNA in the intact central nervous system. Taken together, this study provides the first direct evidence that NTFs functioning via RTKs activate the MEN1 gene, which appears sufficient to regulate synapse formation between central neurons. Our study also offers a novel developmental role for menin beyond tumour suppression in adult humans. PMID:25347295

Resource Guide for Search and Rescue Training Materials.

ERIC Educational Resources Information Center

LaValla, Patrick

The bibliography about search and rescue training materials lists booklets, books, manuals, films, papers, periodicals, and pamphlets that treat many aspects of search and rescue situations: general, cave, disaster, and mountain rescues; strategy tactics; communications; knots and ropes; outdoor living; dogs; tracking; map and compass; survival;…

46 CFR 169.517 - Rescue boat.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Rescue boat. 169.517 Section 169.517 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.517 Rescue boat. All vessels certificated for exposed or partially protected waters service must have a suitable motor rescue boat, except...

46 CFR 169.517 - Rescue boat.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Rescue boat. 169.517 Section 169.517 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.517 Rescue boat. All vessels certificated for exposed or partially protected waters service must have a suitable motor rescue boat, except...

46 CFR 169.517 - Rescue boat.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Rescue boat. 169.517 Section 169.517 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.517 Rescue boat. All vessels certificated for exposed or partially protected waters service must have a suitable motor rescue boat, except...

46 CFR 169.517 - Rescue boat.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Rescue boat. 169.517 Section 169.517 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.517 Rescue boat. All vessels certificated for exposed or partially protected waters service must have a suitable motor rescue boat, except...

46 CFR 169.517 - Rescue boat.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Rescue boat. 169.517 Section 169.517 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.517 Rescue boat. All vessels certificated for exposed or partially protected waters service must have a suitable motor rescue boat, except...

46 CFR 11.317 - Requirements to qualify for an STCW endorsement as master of vessels of less than 500 GT limited...

Code of Federal Regulations, 2014 CFR

2014-10-01

... of this subpart. (iii) Proficiency in survival craft and rescue boats other than fast rescue boats (PSC) or proficiency in survival craft and rescue boats other than lifeboats and fast rescue boats (PSC...

The Search for a Permanent Home: Explaining the Organizational Instability of Air Force Rescue

DTIC Science & Technology

2015-06-01

capability became a major concern after the failed attempt to rescue American hostages in Iran in 1979 . The merger of Rescue and SOF in 1983 succeeded in...Participants General Huyser’s opinion was counter to General Ralph Saunders, the Rescue commander from 1974 to 1979 , who was dedicated to...subsequent Rescue commander, Brigadier General Cornelius Nugteren, took command on 29 September 1979 and was more open to the idea of consolidation. In

Post-disaster medical rescue strategy in tropical regions.

PubMed

Li, Xiang-Hui; Hou, Shi-Ke; Zheng, Jing-Chen; Fan, Hao-Jun; Song, Jian-Qi

2012-01-01

Earthquakes, floods, droughts, storms, mudslides, landslides, and forest wild fires are serious threats to human lives and properties. The present study aimed to study the environmental characteristics and pathogenic traits, recapitulate experiences, and augment applications of medical reliefs in tropical regions. Analysis was made on work and projects of emergency medical rescue, based on information and data collected from 3 emergency medical rescue missions of China International Search and Rescue Team to overseas earthquakes and tsunamis aftermaths in tropical disaster regions - Indonesia-Aceh, Indonesia-Yogyakarta, and Haiti-Port au Prince. Shock, infection and heat stroke were frequently encountered in addition to outbreaks of infectious diseases, skin diseases, and diarrhea during post-disaster emergency medical rescue in tropical regions. High temperature, high humidity, and proliferation of microorganisms and parasites are the characteristics of tropical climate that impose strict requirements on the preparation of rescue work including selective team members suitable for a particular rescue mission and the provisioning of medical equipment and life support materials. The overseas rescue mission itself needs a scientific, efficient, simple workflow for providing efficient emergency medical assistance. Since shock and infection are major tasks in post-disaster treatment of severely injured victims in tropical regions, the prevention and diagnosis of hyperthermia, insect-borne infectious diseases, tropic skin diseases, infectious diarrhea, and pest harms of disaster victims and rescue team staff should be emphasized during the rescue operations.

Clinical and economic analysis of rescue intracytoplasmic sperm injection cycles.

PubMed

Shalom-paz, Einat; Alshalati, Jana; Shehata, Fady; Jimenez, Luis; Son, Weon-Young; Holzer, Hananel; Tan, Seang Lin; Almog, Benny

2011-12-01

To identify clinical and embryological factors that may predict success in rescue intracytoplasmic sperm injection (ICSI) cycles (after total fertilization failure has occurred) and to evaluate the cost effectiveness of rescue ICSI strategy. Additionally, follow-up of 20 rescue ICSI pregnancies is reported. Retrospective analysis of total fertilization failure cycles. University-based tertiary medical center. In total, 92 patients who had undergone conventional in-vitro fertilization (IVF) cycles with total fertilization failure were included. The patients were divided into two subgroups: those who conceived through rescue ICSI and those who did not. The pregnant members of the rescue ICSI subgroup were found to be significantly younger (32.9 ± 4.2 vs. 36.3 ± 4.5, respectively, p = 0.0035,) and to have better-quality embryos than those who did not conceive (cumulative embryo score: 38.3 ± 20.4 vs. 29.3 ± 14.7, p = 0.025). Cost effectiveness analysis showed 25% reduction in the cost per live birth when rescue ICSI is compared to cycle cancellation approach. The pregnancies follow-up did not show adverse perinatal outcome. Rescue ICSI is an option for salvaging IVF cycles complicated by total fertilization failure. Success in rescue ICSI was found to be associated with younger age and higher quality of embryos. Furthermore, the cost effectiveness of rescue ICSI in terms of total fertilization failure was found to be worthwhile.

Precision engineering: an evolutionary perspective.

PubMed

Evans, Chris J

2012-08-28

Precision engineering is a relatively new name for a technology with roots going back over a thousand years; those roots span astronomy, metrology, fundamental standards, manufacturing and money-making (literally). Throughout that history, precision engineers have created links across disparate disciplines to generate innovative responses to society's needs and wants. This review combines historical and technological perspectives to illuminate precision engineering's current character and directions. It first provides us a working definition of precision engineering and then reviews the subject's roots. Examples will be given showing the contributions of the technology to society, while simultaneously showing the creative tension between the technological convergence that spurs new directions and the vertical disintegration that optimizes manufacturing economics.

Space Rescue

NASA Technical Reports Server (NTRS)

Muratore, John F.

2007-01-01

Space Rescue has been a topic of speculation for a wide community of people for decades. Astronauts, aerospace engineers, diplomats, medical and rescue professionals, inventors and science fiction writers have all speculated on this problem. Martin Caidin's 1964 novel Marooned dealt with the problems of rescuing a crew stranded in low earth orbit. Legend at the Johnson Space Center says that Caidin's portrayal of a Russian attempt to save the American crew played a pivotal role in convincing the Russians to join the real joint Apollo-Soyuz mission. Space Rescue has been a staple in science fiction television and movies portrayed in programs such as Star Trek, Stargate-SG1 and Space 1999 and movies such as Mission To Mars and Red Planet. As dramatic and as difficult as rescue appears in fictional accounts, in the real world it has even greater drama and greater difficulty. Space rescue is still in its infancy as a discipline and the purpose of this chapter is to describe the issues associated with space rescue and the work done so far in this field. For the purposes of this chapter, the term space rescue will refer to any system which allows for rescue or escape of personnel from situations which endanger human life in a spaceflight operation. This will span the period from crew ingress prior to flight through crew egress postlanding. For the purposes of this chapter, the term primary system will refer to the spacecraft system that a crew is either attempting to escape from or from which an attempt is being made to rescue the crew.

Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides.

PubMed

Rivera-Torres, Natalia; Banas, Kelly; Bialk, Pawel; Bloh, Kevin M; Kmiec, Eric B

2017-01-01

CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by utilizing a CRISPR/Cas9 ribonucleoprotein (RNP) complex to initiate DNA cleavage. The RNP is assembled in vitro and induces a double stranded break at a specific site surrounding the mutant base designated for correction by the ssODN. We use an integrated mutant eGFP gene, bearing a single base change rendering the expressed protein nonfunctional, as a single copy target in HCT 116 cells. We observe significant gene correction activity of the mutant base, promoted by the RNP and single-stranded DNA oligonucleotide with validation through genotypic and phenotypic readout. We demonstrate that all individual components must be present to obtain successful gene editing. Importantly, we examine the genotype of individually sorted corrected and uncorrected clonally expanded cell populations for the mutagenic footprint left by the action of these gene editing tools. While the DNA sequence of the corrected population is exact with no adjacent sequence modification, the uncorrected population exhibits heterogeneous mutagenicity with a wide variety of deletions and insertions surrounding the target site. We designate this type of DNA aberration as on-site mutagenicity. Analyses of two clonal populations bearing specific DNA insertions surrounding the target site, indicate that point mutation repair has occurred at the level of the gene. The phenotype, however, is not rescued because a section of the single-stranded oligonucleotide has been inserted altering the reading frame and generating truncated proteins. These data illustrate the importance of analysing mutagenicity in uncorrected cells. Our results also form the basis of a simple model for point mutation repair directed by a short single-stranded DNA oligonucleotides and CRISPR/Cas9 ribonucleoprotein complex.

Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides

PubMed Central

Rivera-Torres, Natalia; Bialk, Pawel; Bloh, Kevin M.; Kmiec, Eric B.

2017-01-01

CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by utilizing a CRISPR/Cas9 ribonucleoprotein (RNP) complex to initiate DNA cleavage. The RNP is assembled in vitro and induces a double stranded break at a specific site surrounding the mutant base designated for correction by the ssODN. We use an integrated mutant eGFP gene, bearing a single base change rendering the expressed protein nonfunctional, as a single copy target in HCT 116 cells. We observe significant gene correction activity of the mutant base, promoted by the RNP and single-stranded DNA oligonucleotide with validation through genotypic and phenotypic readout. We demonstrate that all individual components must be present to obtain successful gene editing. Importantly, we examine the genotype of individually sorted corrected and uncorrected clonally expanded cell populations for the mutagenic footprint left by the action of these gene editing tools. While the DNA sequence of the corrected population is exact with no adjacent sequence modification, the uncorrected population exhibits heterogeneous mutagenicity with a wide variety of deletions and insertions surrounding the target site. We designate this type of DNA aberration as on-site mutagenicity. Analyses of two clonal populations bearing specific DNA insertions surrounding the target site, indicate that point mutation repair has occurred at the level of the gene. The phenotype, however, is not rescued because a section of the single-stranded oligonucleotide has been inserted altering the reading frame and generating truncated proteins. These data illustrate the importance of analysing mutagenicity in uncorrected cells. Our results also form the basis of a simple model for point mutation repair directed by a short single-stranded DNA oligonucleotides and CRISPR/Cas9 ribonucleoprotein complex. PMID:28052104

78 FR 79010 - Criteria to Certify Coal Mine Rescue Teams

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... to Certify Coal Mine Rescue Teams AGENCY: Mine Safety and Health Administration, Labor. ACTION... updated the coal mine rescue team certification criteria. The Mine Improvement and New Emergency Response... mine operator to certify the qualifications of a coal mine rescue team is that team members are...

46 CFR 108.565 - Stowage of rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Stowage of rescue boats. 108.565 Section 108.565 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.565 Stowage of rescue boats. (a) Rescue boats must be stowed...

46 CFR 108.565 - Stowage of rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Stowage of rescue boats. 108.565 Section 108.565 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.565 Stowage of rescue boats. (a) Rescue boats must be stowed...

46 CFR 108.565 - Stowage of rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Stowage of rescue boats. 108.565 Section 108.565 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.565 Stowage of rescue boats. (a) Rescue boats must be stowed...

Rescue Skills and Techniques.

ERIC Educational Resources Information Center

Defense Civil Preparedness Agency (DOD), Washington, DC.

The guide has been prepared for use as a textbook in rescue training courses at DCPA (Defense Civil Preparedness Agency) approved training schools and is to be used in rescue training programs of State and local governments. The document explains the various types of rescue missions, command structure, the personnel of the operating unit,…

Rescue Manual. Module 4.

ERIC Educational Resources Information Center

Ohio State Univ., Columbus. Instructional Materials Lab.

This learner manual for rescuers covers the current techniques or practices required in the rescue service. The fourth of 10 modules contains 8 chapters: (1) construction and characteristics of rescue rope; (2) knots, bends, and hitches; (3) critical angles; (4) raising systems; (5) rigging; (6) using the brake-bar rack for rope rescue; (7) rope…

33 CFR 150.508 - What are the maintenance and repair requirements for inflatable rescue boats?

Code of Federal Regulations, 2011 CFR

2011-07-01

... repair requirements for inflatable rescue boats? 150.508 Section 150.508 Navigation and Navigable Waters... repair requirements for inflatable rescue boats? The operator must perform the maintenance and repair of inflatable rescue boats according to the manufacturer's instructions. Operational Tests and Inspections...

46 CFR 199.202 - Rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Rescue boats. 199.202 Section 199.202 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.202 Rescue boats... least one rescue boat approved under approval series 160.156 that is equipped as specified in table 199...

46 CFR 117.210 - Rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Rescue boats. 117.210 Section 117.210 Shipping COAST... Number and Type of Survival Craft § 117.210 Rescue boats. (a) Each vessel must carry at least one rescue boat unless the cognizant OCMI determines that: (1) The vessel is sufficiently maneuverable, arranged...

46 CFR 133.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Rescue boat embarkation, launching and recovery...) OFFSHORE SUPPLY VESSELS LIFESAVING SYSTEMS Requirements for All OSVs § 133.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each davit for a rescue boat must be approved under approval series...

46 CFR 199.202 - Rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Rescue boats. 199.202 Section 199.202 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.202 Rescue boats... least one rescue boat approved under approval series 160.156 that is equipped as specified in table 199...

46 CFR 199.202 - Rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Rescue boats. 199.202 Section 199.202 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.202 Rescue boats... least one rescue boat approved under approval series 160.156 that is equipped as specified in table 199...

46 CFR 117.210 - Rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Rescue boats. 117.210 Section 117.210 Shipping COAST... Number and Type of Survival Craft § 117.210 Rescue boats. (a) Each vessel must carry at least one rescue boat unless the cognizant OCMI determines that: (1) The vessel is sufficiently maneuverable, arranged...

46 CFR 199.202 - Rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Rescue boats. 199.202 Section 199.202 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.202 Rescue boats... least one rescue boat approved under approval series 160.156 that is equipped as specified in table 199...

33 CFR 150.508 - What are the maintenance and repair requirements for inflatable rescue boats?

Code of Federal Regulations, 2012 CFR

2012-07-01

... repair requirements for inflatable rescue boats? 150.508 Section 150.508 Navigation and Navigable Waters... repair requirements for inflatable rescue boats? The operator must perform the maintenance and repair of inflatable rescue boats according to the manufacturer's instructions. Operational Tests and Inspections...

33 CFR 150.508 - What are the maintenance and repair requirements for inflatable rescue boats?

Code of Federal Regulations, 2010 CFR

2010-07-01

... repair requirements for inflatable rescue boats? 150.508 Section 150.508 Navigation and Navigable Waters... repair requirements for inflatable rescue boats? The operator must perform the maintenance and repair of inflatable rescue boats according to the manufacturer's instructions. Operational Tests and Inspections...

46 CFR 117.210 - Rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Rescue boats. 117.210 Section 117.210 Shipping COAST... Number and Type of Survival Craft § 117.210 Rescue boats. (a) Each vessel must carry at least one rescue boat unless the cognizant OCMI determines that: (1) The vessel is sufficiently maneuverable, arranged...

33 CFR 150.508 - What are the maintenance and repair requirements for inflatable rescue boats?

Code of Federal Regulations, 2013 CFR

2013-07-01

... repair requirements for inflatable rescue boats? 150.508 Section 150.508 Navigation and Navigable Waters... repair requirements for inflatable rescue boats? The operator must perform the maintenance and repair of inflatable rescue boats according to the manufacturer's instructions. Operational Tests and Inspections...

46 CFR 133.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Rescue boat embarkation, launching and recovery...) OFFSHORE SUPPLY VESSELS LIFESAVING SYSTEMS Requirements for All OSVs § 133.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each davit for a rescue boat must be approved under approval series...

46 CFR 117.210 - Rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Rescue boats. 117.210 Section 117.210 Shipping COAST... Number and Type of Survival Craft § 117.210 Rescue boats. (a) Each vessel must carry at least one rescue boat unless the cognizant OCMI determines that: (1) The vessel is sufficiently maneuverable, arranged...

33 CFR 150.508 - What are the maintenance and repair requirements for inflatable rescue boats?

Code of Federal Regulations, 2014 CFR

2014-07-01

... repair requirements for inflatable rescue boats? 150.508 Section 150.508 Navigation and Navigable Waters... repair requirements for inflatable rescue boats? The operator must perform the maintenance and repair of inflatable rescue boats according to the manufacturer's instructions. Operational Tests and Inspections...

46 CFR 133.160 - Rescue boat embarkation, launching and recovery arrangements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Rescue boat embarkation, launching and recovery...) OFFSHORE SUPPLY VESSELS LIFESAVING SYSTEMS Requirements for All OSVs § 133.160 Rescue boat embarkation, launching and recovery arrangements. (a) Each davit for a rescue boat must be approved under approval series...

46 CFR 199.202 - Rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Rescue boats. 199.202 Section 199.202 Shipping COAST... SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.202 Rescue boats... least one rescue boat approved under approval series 160.156 that is equipped as specified in table 199...

30 CFR 49.4 - Alternative mine rescue capability for special mining conditions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Alternative mine rescue capability for special mining conditions. 49.4 Section 49.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and...

30 CFR 49.4 - Alternative mine rescue capability for special mining conditions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Alternative mine rescue capability for special mining conditions. 49.4 Section 49.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and...

30 CFR 49.4 - Alternative mine rescue capability for special mining conditions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Alternative mine rescue capability for special mining conditions. 49.4 Section 49.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and...

30 CFR 49.11 - Purpose and scope.

Code of Federal Regulations, 2012 CFR

2012-07-01

... TEAMS Mine Rescue Teams for Underground Coal Mines § 49.11 Purpose and scope. (a) This subpart... recovery. (b) The following Table 49.11 summarizes the new requirements for mine rescue teams contained in... Operators and Mine Rescue Teams Requirement Type of mine rescue team Mine-site Composite Contract State...

30 CFR 49.11 - Purpose and scope.

Code of Federal Regulations, 2011 CFR

2011-07-01

... TEAMS Mine Rescue Teams for Underground Coal Mines § 49.11 Purpose and scope. (a) This subpart... recovery. (b) The following Table 49.11 summarizes the new requirements for mine rescue teams contained in... Operators and Mine Rescue Teams Requirement Type of mine rescue team Mine-site Composite Contract State...

30 CFR 49.11 - Purpose and scope.

Code of Federal Regulations, 2010 CFR

2010-07-01

... TEAMS Mine Rescue Teams for Underground Coal Mines § 49.11 Purpose and scope. (a) This subpart... recovery. (b) The following Table 49.11 summarizes the new requirements for mine rescue teams contained in... Operators and Mine Rescue Teams Requirement Type of mine rescue team Mine-site Composite Contract State...

30 CFR 49.11 - Purpose and scope.

Code of Federal Regulations, 2014 CFR

2014-07-01

... TEAMS Mine Rescue Teams for Underground Coal Mines § 49.11 Purpose and scope. (a) This subpart... recovery. (b) The following Table 49.11 summarizes the new requirements for mine rescue teams contained in... Operators and Mine Rescue Teams Requirement Type of mine rescue team Mine-site Composite Contract State...

78 FR 64537 - Agency Information Collection Activities; Submission for OMB Review; Comment Request; Coal Mine...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-29

... for OMB Review; Comment Request; Coal Mine Rescue Teams: Arrangements for Emergency Medical Assistance... Administration (MSHA) sponsored information collection request (ICR) titled, ``Coal Mine Rescue Teams... mine rescue team requirements; reporting to the MSHA alternative mine rescue capability for a small and...

30 CFR 49.11 - Purpose and scope.

Code of Federal Regulations, 2013 CFR

2013-07-01

... TEAMS Mine Rescue Teams for Underground Coal Mines § 49.11 Purpose and scope. (a) This subpart... recovery. (b) The following Table 49.11 summarizes the new requirements for mine rescue teams contained in... Operators and Mine Rescue Teams Requirement Type of mine rescue team Mine-site Composite Contract State...

30 CFR 49.5 - Mine rescue station.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Mine rescue station. 49.5 Section 49.5 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.5 Mine rescue station. (a) Except where alternative compliance is permitted, every operator...

30 CFR 49.5 - Mine rescue station.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Mine rescue station. 49.5 Section 49.5 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.5 Mine rescue station. (a) Except where alternative compliance is permitted, every operator...

78 FR 39531 - Mine Rescue Teams

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-01

... Administration 30 CFR Part 49 Mine Rescue Teams; CFR Correction #0;#0;Federal Register / Vol. 78 , No. 126... Health Administration 30 CFR Part 49 Mine Rescue Teams CFR Correction In Title 30 of the Code of Federal... Teams Type of mine rescue team Requirement Mine-site Composite Contract State-sponsored...

A SOUND SOURCE LOCALIZATION TECHNIQUE TO SUPPORT SEARCH AND RESCUE IN LOUD NOISE ENVIRONMENTS

NASA Astrophysics Data System (ADS)

Yoshinaga, Hiroshi; Mizutani, Koichi; Wakatsuki, Naoto

At some sites of earthquakes and other disasters, rescuers search for people buried under rubble by listening for the sounds which they make. Thus developing a technique to localize sound sources amidst loud noise will support such search and rescue operations. In this paper, we discuss an experiment performed to test an array signal processing technique which searches for unperceivable sound in loud noise environments. Two speakers simultaneously played a noise of a generator and a voice decreased by 20 dB (= 1/100 of power) from the generator noise at an outdoor space where cicadas were making noise. The sound signal was received by a horizontally set linear microphone array 1.05 m in length and consisting of 15 microphones. The direction and the distance of the voice were computed and the sound of the voice was extracted and played back as an audible sound by array signal processing.

Designing Allosteric Control into Enzymes by Chemical Rescue of Structure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deckert, Katelyn; Budiardjo, S. Jimmy; Brunner, Luke C.

2012-08-07

Ligand-dependent activity has been engineered into enzymes for purposes ranging from controlling cell morphology to reprogramming cellular signaling pathways. Where these successes have typically fused a naturally allosteric domain to the enzyme of interest, here we instead demonstrate an approach for designing a de novo allosteric effector site directly into the catalytic domain of an enzyme. This approach is distinct from traditional chemical rescue of enzymes in that it relies on disruption and restoration of structure, rather than active site chemistry, as a means to achieve modulate function. We present two examples, W33G in a {beta}-glycosidase enzyme ({beta}-gly) and W492Gmore » in a {beta}-glucuronidase enzyme ({beta}-gluc), in which we engineer indole-dependent activity into enzymes by removing a buried tryptophan side chain that serves as a buttress for the active site architecture. In both cases, we observe a loss of function, and in both cases we find that the subsequent addition of indole can be used to restore activity. Through a detailed analysis of {beta}-gly W33G kinetics, we demonstrate that this rescued enzyme is fully functionally equivalent to the corresponding wild-type enzyme. We then present the apo and indole-bound crystal structures of {beta}-gly W33G, which together establish the structural basis for enzyme inactivation and rescue. Finally, we use this designed switch to modulate {beta}-glycosidase activity in living cells using indole. Disruption and recovery of protein structure may represent a general technique for introducing allosteric control into enzymes, and thus may serve as a starting point for building a variety of bioswitches and sensors.« less

Lethality of mice bearing a knockout of the Ngly1-gene is partially rescued by the additional deletion of the Engase gene

PubMed Central

Fujihira, Haruhiko; Masahara-Negishi, Yuki; Tamura, Masaru; Huang, Chengcheng; Harada, Yoichiro; Wakana, Shigeharu; Takakura, Daisuke; Kawasaki, Nana; Taniguchi, Naoyuki; Kondoh, Gen; Yamashita, Tadashi; Funakoshi, Yoko; Suzuki, Tadashi

2017-01-01

The cytoplasmic peptide:N-glycanase (Ngly1 in mammals) is a de-N-glycosylating enzyme that is highly conserved among eukaryotes. It was recently reported that subjects harboring mutations in the NGLY1 gene exhibited severe systemic symptoms (NGLY1-deficiency). While the enzyme obviously has a critical role in mammals, its precise function remains unclear. In this study, we analyzed Ngly1-deficient mice and found that they are embryonic lethal in C57BL/6 background. Surprisingly, the additional deletion of the gene encoding endo-β-N-acetylglucosaminidase (Engase), which is another de-N-glycosylating enzyme but leaves a single GlcNAc at glycosylated Asn residues, resulted in the partial rescue of the lethality of the Ngly1-deficient mice. Additionally, we also found that a change in the genetic background of C57BL/6 mice, produced by crossing the mice with an outbred mouse strain (ICR) could partially rescue the embryonic lethality of Ngly1-deficient mice. Viable Ngly1-deficient mice in a C57BL/6 and ICR mixed background, however, showed a very severe phenotype reminiscent of the symptoms of NGLY1-deficiency subjects. Again, many of those defects were strongly suppressed by the additional deletion of Engase in the C57BL/6 and ICR mixed background. The defects observed in Ngly1/Engase-deficient mice (C57BL/6 background) and Ngly1-deficient mice (C57BL/6 and ICR mixed background) closely resembled some of the symptoms of patients with an NGLY1-deficiency. These observations strongly suggest that the Ngly1- or Ngly1/Engase-deficient mice could serve as a valuable animal model for studies related to the pathogenesis of the NGLY1-deficiency, and that cytoplasmic ENGase represents one of the potential therapeutic targets for this genetic disorder. PMID:28426790

Enhanced neuronal glucose transporter expression reveals metabolic choice in a HD Drosophila model.

PubMed

Besson, Marie Thérèse; Alegría, Karin; Garrido-Gerter, Pamela; Barros, Luis Felipe; Liévens, Jean-Charles

2015-01-01

Huntington's disease is a neurodegenerative disorder caused by toxic insertions of polyglutamine residues in the Huntingtin protein and characterized by progressive deterioration of cognitive and motor functions. Altered brain glucose metabolism has long been suggested and a possible link has been proposed in HD. However, the precise function of glucose transporters was not yet determined. Here, we report the effects of the specifically-neuronal human glucose transporter expression in neurons of a Drosophila model carrying the exon 1 of the human huntingtin gene with 93 glutamine repeats (HQ93). We demonstrated that overexpression of the human glucose transporter in neurons ameliorated significantly the status of HD flies by increasing their lifespan, reducing their locomotor deficits and rescuing eye neurodegeneration. Then, we investigated whether increasing the major pathways of glucose catabolism, glycolysis and pentose-phosphate pathway (PPP) impacts HD. To mimic increased glycolytic flux, we overexpressed phosphofructokinase (PFK) which catalyzes an irreversible step in glycolysis. Overexpression of PFK did not affect HQ93 fly survival, but protected from photoreceptor loss. Overexpression of glucose-6-phosphate dehydrogenase (G6PD), the key enzyme of the PPP, extended significantly the lifespan of HD flies and rescued eye neurodegeneration. Since G6PD is able to synthesize NADPH involved in cell survival by maintenance of the redox state, we showed that tolerance to experimental oxidative stress was enhanced in flies co-expressing HQ93 and G6PD. Additionally overexpressions of hGluT3, G6PD or PFK were able to circumvent mitochondrial deficits induced by specific silencing of genes necessary for mitochondrial homeostasis. Our study confirms the involvement of bioenergetic deficits in HD course; they can be rescued by specific expression of a glucose transporter in neurons. Finally, the PPP and, to a lesser extent, the glycolysis seem to mediate the hGluT3 protective effects, whereas, in addition, the PPP provides increased protection to oxidative stress.

30 CFR 49.50 - Certification of coal mine rescue teams.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Certification of coal mine rescue teams. 49.50... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.50 Certification of coal mine... coal mine, the mine operator shall send the District Manager an annual statement certifying that each...

30 CFR 49.50 - Certification of coal mine rescue teams.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Certification of coal mine rescue teams. 49.50... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.50 Certification of coal mine... coal mine, the mine operator shall send the District Manager an annual statement certifying that each...

30 CFR 49.50 - Certification of coal mine rescue teams.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Certification of coal mine rescue teams. 49.50... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.50 Certification of coal mine... coal mine, the mine operator shall send the District Manager an annual statement certifying that each...

30 CFR 49.50 - Certification of coal mine rescue teams.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Certification of coal mine rescue teams. 49.50... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.50 Certification of coal mine... coal mine, the mine operator shall send the District Manager an annual statement certifying that each...

30 CFR 49.50 - Certification of coal mine rescue teams.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Certification of coal mine rescue teams. 49.50... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.50 Certification of coal mine... coal mine, the mine operator shall send the District Manager an annual statement certifying that each...

30 CFR 57.15031 - Location of self-rescue devices.

Code of Federal Regulations, 2010 CFR

2010-07-01

... around mobile equipment, self-rescue devices may be placed in a readily accessible location on such... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Location of self-rescue devices. 57.15031... Protection Underground Only § 57.15031 Location of self-rescue devices. (a) Except as provided in paragraph...

33 CFR 150.504 - When must the operator service and examine lifeboat and rescue boat launching appliances?

Code of Federal Regulations, 2012 CFR

2012-07-01

... and examine lifeboat and rescue boat launching appliances? 150.504 Section 150.504 Navigation and... and examine lifeboat and rescue boat launching appliances? (a) The operator must service launching appliances for lifeboats and rescue boats at intervals recommended in the manufacturer's instructions under...

33 CFR 150.504 - When must the operator service and examine lifeboat and rescue boat launching appliances?

Code of Federal Regulations, 2013 CFR

2013-07-01

... and examine lifeboat and rescue boat launching appliances? 150.504 Section 150.504 Navigation and... and examine lifeboat and rescue boat launching appliances? (a) The operator must service launching appliances for lifeboats and rescue boats at intervals recommended in the manufacturer's instructions under...

46 CFR 180.210 - Rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Rescue boats. 180.210 Section 180.210 Shipping COAST...) LIFESAVING EQUIPMENT AND ARRANGEMENTS Number and Type of Survival Craft § 180.210 Rescue boats. (a) A vessel of more than 19.8 meters (65 feet) in length must carry at least one rescue boat unless the cognizant...

33 CFR 150.504 - When must the operator service and examine lifeboat and rescue boat launching appliances?

Code of Federal Regulations, 2010 CFR

2010-07-01

... and examine lifeboat and rescue boat launching appliances? 150.504 Section 150.504 Navigation and... and examine lifeboat and rescue boat launching appliances? (a) The operator must service launching appliances for lifeboats and rescue boats at intervals recommended in the manufacturer's instructions under...

46 CFR 133.175 - Survival craft and rescue boat equipment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Survival craft and rescue boat equipment. 133.175... LIFESAVING SYSTEMS Requirements for All OSVs § 133.175 Survival craft and rescue boat equipment. (a) All rescue boat equipment must be as follows: (1) The equipment must be secured within the boat by lashings...

33 CFR 150.504 - When must the operator service and examine lifeboat and rescue boat launching appliances?

Code of Federal Regulations, 2014 CFR

2014-07-01

... and examine lifeboat and rescue boat launching appliances? 150.504 Section 150.504 Navigation and... and examine lifeboat and rescue boat launching appliances? (a) The operator must service launching appliances for lifeboats and rescue boats at intervals recommended in the manufacturer's instructions under...

46 CFR 108.575 - Survival craft and rescue boat equipment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Survival craft and rescue boat equipment. 108.575... DRILLING UNITS DESIGN AND EQUIPMENT Lifesaving Equipment § 108.575 Survival craft and rescue boat equipment. (a) All lifeboat and rescue boat equipment must be as follows: (1) The equipment must be secured...

46 CFR 133.175 - Survival craft and rescue boat equipment.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Survival craft and rescue boat equipment. 133.175... LIFESAVING SYSTEMS Requirements for All OSVs § 133.175 Survival craft and rescue boat equipment. (a) All rescue boat equipment must be as follows: (1) The equipment must be secured within the boat by lashings...

33 CFR 150.504 - When must the operator service and examine lifeboat and rescue boat launching appliances?

Code of Federal Regulations, 2011 CFR

2011-07-01

... and examine lifeboat and rescue boat launching appliances? 150.504 Section 150.504 Navigation and... and examine lifeboat and rescue boat launching appliances? (a) The operator must service launching appliances for lifeboats and rescue boats at intervals recommended in the manufacturer's instructions under...

46 CFR 180.210 - Rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Rescue boats. 180.210 Section 180.210 Shipping COAST...) LIFESAVING EQUIPMENT AND ARRANGEMENTS Number and Type of Survival Craft § 180.210 Rescue boats. (a) A vessel of more than 19.8 meters (65 feet) in length must carry at least one rescue boat unless the cognizant...

46 CFR 180.210 - Rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Rescue boats. 180.210 Section 180.210 Shipping COAST...) LIFESAVING EQUIPMENT AND ARRANGEMENTS Number and Type of Survival Craft § 180.210 Rescue boats. (a) A vessel of more than 19.8 meters (65 feet) in length must carry at least one rescue boat unless the cognizant...

46 CFR 180.210 - Rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Rescue boats. 180.210 Section 180.210 Shipping COAST...) LIFESAVING EQUIPMENT AND ARRANGEMENTS Number and Type of Survival Craft § 180.210 Rescue boats. (a) A vessel of more than 19.8 meters (65 feet) in length must carry at least one rescue boat unless the cognizant...

46 CFR 133.175 - Survival craft and rescue boat equipment.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Survival craft and rescue boat equipment. 133.175... LIFESAVING SYSTEMS Requirements for All OSVs § 133.175 Survival craft and rescue boat equipment. (a) All rescue boat equipment must be as follows: (1) The equipment must be secured within the boat by lashings...

46 CFR 133.175 - Survival craft and rescue boat equipment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Survival craft and rescue boat equipment. 133.175... LIFESAVING SYSTEMS Requirements for All OSVs § 133.175 Survival craft and rescue boat equipment. (a) All rescue boat equipment must be as follows: (1) The equipment must be secured within the boat by lashings...

75 FR 18888 - Mine Rescue Teams and Arrangements for Emergency Medical Assistance and Transportation for...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-13

... DEPARTMENT OF LABOR Mine Safety and Health Administration Mine Rescue Teams and Arrangements for... revising the Agency's requirements for mine rescue teams for underground coal mines on February 8, 2008... provisions. Consistent with the Court's decision, MSHA revised its requirements for mine rescue teams for...

30 CFR 49.4 - Alternative mine rescue capability for special mining conditions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Alternative mine rescue capability for special mining conditions. 49.4 Section 49.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.4 Alternative mine rescue capability for...

30 CFR 49.2 - Availability of mine rescue teams.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Availability of mine rescue teams. 49.2 Section... TRAINING MINE RESCUE TEAMS § 49.2 Availability of mine rescue teams. (a) Except where alternative... teams which are available at all times when miners are underground; or (2) Enter into an arrangement for...

30 CFR 49.4 - Alternative mine rescue capability for special mining conditions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Alternative mine rescue capability for special mining conditions. 49.4 Section 49.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR EDUCATION AND TRAINING MINE RESCUE TEAMS § 49.4 Alternative mine rescue capability for...

30 CFR 49.2 - Availability of mine rescue teams.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Availability of mine rescue teams. 49.2 Section... TRAINING MINE RESCUE TEAMS § 49.2 Availability of mine rescue teams. (a) Except where alternative... teams which are available at all times when miners are underground; or (2) Enter into an arrangement for...

46 CFR 12.10-9 - Endorsement for proficiency in fast rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Endorsement for proficiency in fast rescue boats. 12.10... SEAMEN REQUIREMENTS FOR RATING ENDORSEMENTS Lifeboatman § 12.10-9 Endorsement for proficiency in fast rescue boats. (a) Each person engaged or employed as a lifeboatman proficient in fast rescue boats must...

46 CFR 12.10-9 - Endorsement for proficiency in fast rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Endorsement for proficiency in fast rescue boats. 12.10... SEAMEN REQUIREMENTS FOR RATING ENDORSEMENTS Lifeboatman § 12.10-9 Endorsement for proficiency in fast rescue boats. (a) Each person engaged or employed as a lifeboatman proficient in fast rescue boats must...

46 CFR 12.10-9 - Endorsement for proficiency in fast rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Endorsement for proficiency in fast rescue boats. 12.10... SEAMEN REQUIREMENTS FOR RATING ENDORSEMENTS Lifeboatman § 12.10-9 Endorsement for proficiency in fast rescue boats. (a) Each person engaged or employed as a lifeboatman proficient in fast rescue boats must...

46 CFR 12.10-9 - Endorsement for proficiency in fast rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Endorsement for proficiency in fast rescue boats. 12.10... SEAMEN REQUIREMENTS FOR RATING ENDORSEMENTS Lifeboatman § 12.10-9 Endorsement for proficiency in fast rescue boats. (a) Each person engaged or employed as a lifeboatman proficient in fast rescue boats must...

46 CFR 160.056-4 - Approval tests of prototype rescue boat.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 6 2010-10-01 2010-10-01 false Approval tests of prototype rescue boat. 160.056-4 Section 160.056-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT... tests of prototype rescue boat. (a) Drop test. The rescue boat, fully equipped, shall be dropped, in a...

The wildlife research & rescue programme for mammals at Hulu Terengganu Hydroelectric Project (HTHEP), Terengganu, Peninsular Malaysia

NASA Astrophysics Data System (ADS)

Nur-Syuhada, N.; Magintan, D.; Siti-Hajar, A. R.; Aisah, M. S.; Shukor, M. N.

2016-11-01

During the inundation of the Hulu Terengganu Hydroelectric Project in October 2014, a wildlife rescue programme was conducted to rescue animals found trapped within the expanding 61.6 km2 reservoir. A total of 244 mammals from 30 species, representing 12 families were rescued by various methods included baited live trapping, catchpoles, hoop nets and by hand. The order Rodentia recorded the highest amount of rescued individuals at 20.9%, followed by Primate (18.9), Dermoptera (11.1), Carnivora (0.8) and Pholidota (0.4). The genus Rattus recorded the highest individuals rescued (51 individuals) probably due to the rapid clear-cut logging of the forest prior to inundation. Notable mammals of high conservational value rescued included Manis javanicus (Pangolin), Presbytis melalophos siamensis (Mitred Leaf Monkey), Trachypithecus obscurus (Dusky Leaf Monkey), Hylobates lar (White Handed Gibbon), Nycticebus coucang (Slow Loris), Galeopterus variegatus (Sunda Colugo), Callosciurus nigrovittatus (Sunda Black-banded Squirrel), Ratufa spp. (Giant Squirrels), and Sundasciurus hippurus (Horse-tailed Squirrel). Various data and biological samples were collected from the mammals rescued prior to their release at the nearest forest reserves. Rescue operation enabled the inventory and comprehensive data collection of various arboreal and rare mammal species that are hard to capture using the traditional survey method.

76 FR 28169 - Airworthiness Directives; Agusta S.p.A. Model AB412 Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-16

... lock pin may cause the loss of the hoist hook and any load. The absence of the lock pin constitutes an... cause the loss of the hoist hook and any load. The absence of the lock pin constitutes an unsafe... absence of this lock pin to prevent the loss of a rescue hoist hook and its load. [[Page 28171

[Medical rescue of China National Earthquake Disaster Emergency Search and Rescue Team in Lushan earthquake].

PubMed

Liu, Ya-hua; Yang, Hui-ning; Liu, Hui-liang; Wang, Fan; Hu, Li-bin; Zheng, Jing-chen

2013-05-01

To summarize and analyze the medical mission of China National Earthquake Disaster Emergency Search and Rescue Team (CNESAR) in Lushan earthquake, to promote the medical rescue effectiveness incorporated with search and rescue. Retrospective analysis of medical work data by CNESAR from April 21th, 2013 to April 27th during Lushan earthquake rescue, including the medical staff dispatch and the wounded case been treated. The reasonable medical corps was composed by 22 members, including 2 administrators, 11 doctors [covering emergency medicine, orthopedics (joints and limbs, spinal), obstetrics and gynecology, gastroenterology, cardiology, ophthalmology, anesthesiology, medical rescue, health epidemic prevention, clinical laboratory of 11 specialties], 1 ultrasound technician, 5 nurses, 1 pharmacist, 1 medical instrument engineer and 1 office worker for propaganda. There were two members having psychological consultants qualifications. The medical work were carried out in seven aspects, including medical care assurance for the CNESAR members, first aid cooperation with search and rescue on site, clinical work in refugees' camp, medical round service for scattered village people, evacuation for the wounded, mental intervention, and the sanitary and anti-epidemic work. The medical work covered 24 small towns, and medical staff established 3 medical clinics at Taiping Town, Shuangshi Town of Lushan County and Baoxing County. Medical rescue, mental intervention for the old and kids, and sanitary and anti-epidemic were performed at the above sites. The medical corps had successful evacuated 2 severe wounded patients and treated the wounded over thousands. Most of the wounded were soft tissue injuries, external injury, respiratory tract infections, diarrhea, and heat stroke. Compared with the rescue action in 2008 Wenchuan earthquake, the aggregation and departure of rescue team in Lushan earthquake, the traffic control order in disaster area, the self-aid and buddy aid are better, which give rise to the casualties to the lowest. The medical mission incorporated with search and rescue work showed that the medical performance manner altered with stages, the medical staff match changed with the mission, and the focus related with rescue time.

Plasmacytoid Dendritic Cells Require Direct Infection To Sustain the Pulmonary Influenza A Virus-Specific CD8 T Cell Response

PubMed Central

Hemann, Emily A.; Sjaastad, Louisa E.; Langlois, Ryan A.

2015-01-01

ABSTRACT Following influenza A virus (IAV) infection, development of a robust IAV-specific CD8 T cell response is required for clearance of primary infection and enhances memory protection. Following IAV infection, plasmacytoid dendritic cells (pDC) or CD8α+ DC regulate pulmonary effector CD8 T cell responses within the lung. Without this DC-T cell interaction, insufficient effector CD8 T cells are maintained in the lungs, leading to enhanced morbidity and mortality. Previous studies have demonstrated that pDC are capable of classical presentation or cross-presentation of IAV antigens and could potentially regulate IAV-specific CD8 T cell responses through either mechanism. Our results demonstrate that pDC from the lungs of donor mice infected with an IAV that is not able to replicate in hematopoietic cells (142t-IAV), unlike donor pDC isolated from the lungs of control infected mice, are not able to rescue the host IAV-specific CD8 T cell response from apoptosis. This indicates that pDC must utilize the direct presentation pathway for this rescue. This inability of pDC from 142t-IAV donors to rescue the IAV-specific CD8 T cell response is not due to differences in the overall ability of 142t-IAV to replicate within the lungs or generate defective viral genomes or to differences in levels of costimulatory molecules required for this interaction. We further demonstrate that bypassing the antigen presentation pathway by coating the 142t-IAV pDC with IAV peptide epitopes restores their ability to rescue the IAV-specific CD8 T cell response. IMPORTANCE IAV continues to be a global health burden, infecting 5 to 20% of the global population annually. Continued investigation into the mechanisms that mediate protective immune responses against IAV is important to improving current vaccination and antiviral strategies antagonistic toward IAV. Our findings presented herein demonstrate a key requirement for pDC promotion of effector CD8 T cell survival: that rather than utilizing cross-presentation, pDC must be infected and utilize the endogenous pathway for presentation of antigens to CD8 T cells during in vivo IAV infections. This suggests that targeting presentation via the endogenous pathway in pDC could be important for the development of unique antiviral cellular therapies. PMID:26719269

Maryland Fire-Rescue Education and Training System. Organizational Design.

ERIC Educational Resources Information Center

Maryland Fire-Rescue Education and Training Commission.

This is a description of the Maryland system which was created to evaluate local fire-rescue education and training needs and capabilities and to assist local authorities with fire-rescue education and training. In the first of four parts, an historical presentation is used to identify and describe in general terms the state fire, rescue, and…

46 CFR 111.75-16 - Lighting of survival craft and rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Lighting of survival craft and rescue boats. 111.75-16... craft and rescue boats. (a) During preparation, launching, and recovery, each survival craft and rescue boat, its launching appliance, and the area of water into which it is to be launched or recovered must...

46 CFR 111.75-16 - Lighting of survival craft and rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Lighting of survival craft and rescue boats. 111.75-16... craft and rescue boats. (a) During preparation, launching, and recovery, each survival craft and rescue boat, its launching appliance, and the area of water into which it is to be launched or recovered must...

33 CFR 150.505 - When must the operator service and examine lifeboat and rescue boat release gear?

Code of Federal Regulations, 2013 CFR

2013-07-01

... and examine lifeboat and rescue boat release gear? 150.505 Section 150.505 Navigation and Navigable... and examine lifeboat and rescue boat release gear? (a) The operator must service lifeboat and rescue boat release gear at intervals recommended in the manufacturer's instructions under § 150.502(a), or...

46 CFR 131.855 - Lifeboats and rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Lifeboats and rescue boats. 131.855 Section 131.855... Markings for Fire Equipment and Emergency Equipment § 131.855 Lifeboats and rescue boats. (a) The following must be plainly marked or painted on each side of the bow of each lifeboat and rescue boat in block...

46 CFR 199.140 - Stowage of rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Stowage of rescue boats. 199.140 Section 199.140... boats. (a) General. Rescue boats must be stowed— (1) To be ready for launching in not more than 5 minutes. (2) In a position suitable for launching and recovery; (3) In a way that neither the rescue boat...

33 CFR 150.505 - When must the operator service and examine lifeboat and rescue boat release gear?

Code of Federal Regulations, 2011 CFR

2011-07-01

... and examine lifeboat and rescue boat release gear? 150.505 Section 150.505 Navigation and Navigable... and examine lifeboat and rescue boat release gear? (a) The operator must service lifeboat and rescue boat release gear at intervals recommended in the manufacturer's instructions under § 150.502(a), or...

33 CFR 150.505 - When must the operator service and examine lifeboat and rescue boat release gear?

Code of Federal Regulations, 2014 CFR

2014-07-01

... and examine lifeboat and rescue boat release gear? 150.505 Section 150.505 Navigation and Navigable... and examine lifeboat and rescue boat release gear? (a) The operator must service lifeboat and rescue boat release gear at intervals recommended in the manufacturer's instructions under § 150.502(a), or...

46 CFR 131.855 - Lifeboats and rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Lifeboats and rescue boats. 131.855 Section 131.855... Markings for Fire Equipment and Emergency Equipment § 131.855 Lifeboats and rescue boats. (a) The following must be plainly marked or painted on each side of the bow of each lifeboat and rescue boat in block...

46 CFR 111.75-16 - Lighting of survival craft and rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Lighting of survival craft and rescue boats. 111.75-16... craft and rescue boats. (a) During preparation, launching, and recovery, each survival craft and rescue boat, its launching appliance, and the area of water into which it is to be launched or recovered must...

46 CFR 131.855 - Lifeboats and rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Lifeboats and rescue boats. 131.855 Section 131.855... Markings for Fire Equipment and Emergency Equipment § 131.855 Lifeboats and rescue boats. (a) The following must be plainly marked or painted on each side of the bow of each lifeboat and rescue boat in block...

46 CFR 131.855 - Lifeboats and rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Lifeboats and rescue boats. 131.855 Section 131.855... Markings for Fire Equipment and Emergency Equipment § 131.855 Lifeboats and rescue boats. (a) The following must be plainly marked or painted on each side of the bow of each lifeboat and rescue boat in block...

33 CFR 150.505 - When must the operator service and examine lifeboat and rescue boat release gear?

Code of Federal Regulations, 2012 CFR

2012-07-01

... and examine lifeboat and rescue boat release gear? 150.505 Section 150.505 Navigation and Navigable... and examine lifeboat and rescue boat release gear? (a) The operator must service lifeboat and rescue boat release gear at intervals recommended in the manufacturer's instructions under § 150.502(a), or...

46 CFR 131.855 - Lifeboats and rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Lifeboats and rescue boats. 131.855 Section 131.855... Markings for Fire Equipment and Emergency Equipment § 131.855 Lifeboats and rescue boats. (a) The following must be plainly marked or painted on each side of the bow of each lifeboat and rescue boat in block...

46 CFR 199.140 - Stowage of rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Stowage of rescue boats. 199.140 Section 199.140... boats. (a) General. Rescue boats must be stowed— (1) To be ready for launching in not more than 5 minutes. (2) In a position suitable for launching and recovery; (3) In a way that neither the rescue boat...

46 CFR 199.140 - Stowage of rescue boats.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Stowage of rescue boats. 199.140 Section 199.140... boats. (a) General. Rescue boats must be stowed— (1) To be ready for launching in not more than 5 minutes. (2) In a position suitable for launching and recovery; (3) In a way that neither the rescue boat...

46 CFR 199.140 - Stowage of rescue boats.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Stowage of rescue boats. 199.140 Section 199.140... boats. (a) General. Rescue boats must be stowed— (1) To be ready for launching in not more than 5 minutes. (2) In a position suitable for launching and recovery; (3) In a way that neither the rescue boat...

46 CFR 111.75-16 - Lighting of survival craft and rescue boats.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Lighting of survival craft and rescue boats. 111.75-16... craft and rescue boats. (a) During preparation, launching, and recovery, each survival craft and rescue boat, its launching appliance, and the area of water into which it is to be launched or recovered must...

46 CFR 199.140 - Stowage of rescue boats.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Stowage of rescue boats. 199.140 Section 199.140... boats. (a) General. Rescue boats must be stowed— (1) To be ready for launching in not more than 5 minutes. (2) In a position suitable for launching and recovery; (3) In a way that neither the rescue boat...

33 CFR 150.505 - When must the operator service and examine lifeboat and rescue boat release gear?

Code of Federal Regulations, 2010 CFR

2010-07-01

... and examine lifeboat and rescue boat release gear? 150.505 Section 150.505 Navigation and Navigable... and examine lifeboat and rescue boat release gear? (a) The operator must service lifeboat and rescue boat release gear at intervals recommended in the manufacturer's instructions under § 150.502(a), or...

30 CFR 49.60 - Requirements for a local mine rescue contest.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.60 Requirements... United States; (2) Uses MSHA-recognized rules; (3) Has a minimum of three mine rescue teams competing; (4) Has one or more problems conducted on one or more days with a determined winner; (5) Includes team...

30 CFR 49.60 - Requirements for a local mine rescue contest.

Code of Federal Regulations, 2013 CFR

2013-07-01

... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.60 Requirements... United States; (2) Uses MSHA-recognized rules; (3) Has a minimum of three mine rescue teams competing; (4) Has one or more problems conducted on one or more days with a determined winner; (5) Includes team...

30 CFR 49.60 - Requirements for a local mine rescue contest.

Code of Federal Regulations, 2012 CFR

2012-07-01

... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.60 Requirements... United States; (2) Uses MSHA-recognized rules; (3) Has a minimum of three mine rescue teams competing; (4) Has one or more problems conducted on one or more days with a determined winner; (5) Includes team...

30 CFR 49.60 - Requirements for a local mine rescue contest.

Code of Federal Regulations, 2011 CFR

2011-07-01

... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.60 Requirements... United States; (2) Uses MSHA-recognized rules; (3) Has a minimum of three mine rescue teams competing; (4) Has one or more problems conducted on one or more days with a determined winner; (5) Includes team...

30 CFR 49.60 - Requirements for a local mine rescue contest.

Code of Federal Regulations, 2014 CFR

2014-07-01

... EDUCATION AND TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.60 Requirements... United States; (2) Uses MSHA-recognized rules; (3) Has a minimum of three mine rescue teams competing; (4) Has one or more problems conducted on one or more days with a determined winner; (5) Includes team...

Lunar mission safety and rescue: Technical summary

NASA Technical Reports Server (NTRS)

1971-01-01

A technical summary is presented of the escape/rescue and the hazards analyses for manned missions and operations in the 1980 time frame. Hazards are interpreted as hazards to man, not to equipment, program schedule, or program objectives. Hazards in 39 individual areas are analyzed, and corrective measures are recommended. Over 200 safety guidelines are proposed, based on significant hazards. Escape and rescue situtations and requirements are identified and analyzed, and escape/survival/rescue concepts are defined to cope with each escape/rescue situation. Areas in which research or technical development efforts could improve mission safety are identified. It is concluded that the primary emphasis should be on survival and escape provisions, with rescue required only where self-help cannot bring the endangered crewmen to a safe haven.

[Development and application of emergency medical information management system].

PubMed

Wang, Fang; Zhu, Baofeng; Chen, Jianrong; Wang, Jian; Gu, Chaoli; Liu, Buyun

2011-03-01

To meet the needs of clinical practice of rescuing critical illness and develop the information management system of the emergency medicine. Microsoft Visual FoxPro, which is one of Microsoft's visual programming tool, is used to develop computer-aided system included the information management system of the emergency medicine. The system mainly consists of the module of statistic analysis, the module of quality control of emergency rescue, the module of flow path of emergency rescue, the module of nursing care in emergency rescue, and the module of rescue training. It can realize the system management of emergency medicine and,process and analyze the emergency statistical data. This system is practical. It can optimize emergency clinical pathway, and meet the needs of clinical rescue.

Straight as an arrow: humpback whales swim constant course tracks during long-distance migration

PubMed Central

Horton, Travis W.; Holdaway, Richard N.; Zerbini, Alexandre N.; Hauser, Nan; Garrigue, Claire; Andriolo, Artur; Clapham, Phillip J.

2011-01-01

Humpback whale seasonal migrations, spanning greater than 6500 km of open ocean, demonstrate remarkable navigational precision despite following spatially and temporally distinct migration routes. Satellite-monitored radio tag-derived humpback whale migration tracks in both the South Atlantic and South Pacific include constant course segments of greater than 200 km, each spanning several days of continuous movement. The whales studied here maintain these directed movements, often with better than 1° precision, despite the effects of variable sea-surface currents. Such remarkable directional precision is difficult to explain by established models of directional orientation, suggesting that alternative compass mechanisms should be explored. PMID:21508023

Straight as an arrow: humpback whales swim constant course tracks during long-distance migration.

PubMed

Horton, Travis W; Holdaway, Richard N; Zerbini, Alexandre N; Hauser, Nan; Garrigue, Claire; Andriolo, Artur; Clapham, Phillip J

2011-10-23

Humpback whale seasonal migrations, spanning greater than 6500 km of open ocean, demonstrate remarkable navigational precision despite following spatially and temporally distinct migration routes. Satellite-monitored radio tag-derived humpback whale migration tracks in both the South Atlantic and South Pacific include constant course segments of greater than 200 km, each spanning several days of continuous movement. The whales studied here maintain these directed movements, often with better than 1° precision, despite the effects of variable sea-surface currents. Such remarkable directional precision is difficult to explain by established models of directional orientation, suggesting that alternative compass mechanisms should be explored.

Apparatus for correcting precision errors in slide straightness in machine tools

DOEpatents

Robinson, Samuel C.; Gerth, Howard L.

1981-01-01

The present invention is directed to a mechanism by which small deviations in slideway straightness and roll of a precision machining apparatus may be compensated for. The mechanism of the present invention comprises a fixture support disposed between the slideway carriage and the tool or workpiece fixture and provided with a hinge-like coupling between the carriage and the fixture support so as to allow for the minute and precise displacement of the fixture support in a direction normal to the direction of the slide path so as to readily compensate for slight deviations in the straightness and roll of the slide path.

Apparatus for correcting precision errors in slide straigntness in machine tools

DOEpatents

Robinson, S.C.; Gerth, H.L.

The present invention is directed to a mechanism by which small deviations in slideway straightness and roll of a precision machining apparatus may be compensated for. The mechanism of the present invention comprises a fixture support disposed between the slideway carriage and the tool or workpiece fixture and provided with a hinge-like coupling between the carriage and the fixture support so as to allow for the minute and precise displacement of the fixture support in a direction normal to the direction of the slide path soa as to readily compensate for slight deviations in the straightness and roll of the slide path.

Physical capacity of rescue personnel in the mining industry

PubMed Central

Stewart, Ian B; McDonald, Michael D; Hunt, Andrew P; Parker, Tony W

2008-01-01

Background The mining industry has one of the highest occupational rates of serious injury and fatality. Mine staff involved with rescue operations are often required to respond to physically challenging situations. This paper describes the physical attributes of mining rescue personnel. Methods 91 rescue personnel (34 ± 8.6 yrs, 1.79 ± 0.07 m, 90 ± 15.0 kg) participating in the Queensland Mines Rescue Challenge completed a series of health-related and rescue-related fitness tasks. Health-related tasks comprised measurements of aerobic capacity (VO2max), abdominal endurance, abdominal strength, flexibility, lower back strength, leg strength, elbow flexion strength, shoulder strength, lower back endurance, and leg endurance. Rescue-related tasks comprised an incremental carry (IC), coal shovel (CS), and a hose drag (HD), completed in this order. Results Cardiovascular (VO2max) and muscular endurance was average or below average compared with the general population. Isometric strength did not decline with age. The rescue-related tasks were all extremely demanding with heart rate responses averaging greater than 88% of age predicted maximal heart rates. Heart rate recovery responses were more discriminating than heart rates recorded during the tasks, indicating the hose drag as the most physically demanding of the tasks. Conclusion Relying on actual rescues or mining related work to provide adequate training is generally insufficient to maintain, let alone increase, physical fitness. It is therefore recommended that standards of required physical fitness be developed and mines rescue personnel undergo regularly training (and assessment) in order to maintain these standards. PMID:18847510

Analysis of the pre-incident education and subsequent performance of emergency medical responders to the Volendam café fire.

PubMed

Welling, Lieke; Perez, Roberto S G M; van Harten, Sabine M; Patka, Peter; Mackie, Dave P; Kreis, Robert W; Bierens, Joost J L M

2005-12-01

At this moment, in the Netherlands, rescue workers are not given any specific standardized training in disaster response or disaster management. After the café fire in Volendam, the Netherlands, on New Year's Eve 2000, around 200 rescue workers were deployed on-site. The aim of this study is to investigate the rescue workers' experiences with regard to their level of preparation for the emergency response. In 2002, 30 members of the medical and paramedical personnel were requested to participate in a structured interview, focused on education, task perception, triage and registration. Twenty-seven participated. Twenty-two rescue workers received previous training in emergency medicine. During the alarm phase, 11 rescue workers had a clear perception of their tasks. Twenty-four were involved in triage and injury assessment. Three rescue workers used a protocol for triage and 15 for injury assessment. Twenty-five rescue workers gave on-scene treatment and 15 used a protocol. Eight registered their findings. Preparation for the emergency response lacked standardized procedures. The use of triage protocols was extremely poor, as was documentation of actions. Slightly more than half of the personnel followed treatment protocols. It is advisable that all rescue workers become familiar with the basic uniform principles and protocols regarding disaster management. A dedicated and standardized national disaster management course is needed for all rescue workers.

46 CFR 160.156-15 - Production inspections, tests, quality control, and conformance of rescue boats and fast rescue...

Code of Federal Regulations, 2012 CFR

2012-10-01

... materials are as per the plans approved under § 160.156-13(h) of this subpart. (2) Post assembly tests and... 46 Shipping 6 2012-10-01 2012-10-01 false Production inspections, tests, quality control, and... inspections, tests, quality control, and conformance of rescue boats and fast rescue boats. (a) Unless the...

46 CFR 160.156-15 - Production inspections, tests, quality control, and conformance of rescue boats and fast rescue...

Code of Federal Regulations, 2013 CFR

2013-10-01

... materials are as per the plans approved under § 160.156-13(h) of this subpart. (2) Post assembly tests and... 46 Shipping 6 2013-10-01 2013-10-01 false Production inspections, tests, quality control, and... inspections, tests, quality control, and conformance of rescue boats and fast rescue boats. (a) Unless the...

46 CFR 12.617 - Requirements to qualify for an STCW endorsement in proficiency in fast rescue boats.

Code of Federal Regulations, 2014 CFR

2014-10-01

... proficiency in fast rescue boats. 12.617 Section 12.617 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Endorsements § 12.617 Requirements to qualify for an STCW endorsement in proficiency in fast rescue boats. (a) To qualify for an STCW endorsement in proficiency in fast rescue boats, an applicant must— (1) Be not...

A new approach to road accident rescue.

PubMed

Morales, Alejandro; González-Aguilera, Diego; López, Alfonso I; Gutiérrez, Miguel A

2016-01-01

This article develops and validates a new methodology and tool for rescue assistance in traffic accidents, with the aim of improving its efficiency and safety in the evacuation of people, reducing the number of victims in road accidents. Different tests supported by professionals and experts have been designed under different circumstances and with different categories of damaged vehicles coming from real accidents and simulated trapped victims in order to calibrate and refine the proposed methodology and tool. To validate this new approach, a tool called App_Rescue has been developed. This tool is based on the use of a computer system that allows an efficient access to the technical information of the vehicle and sanitary information of the common passengers. The time spent during rescue using the standard protocol and the proposed method was compared. This rescue assistance system allows us to make vital information accessible in posttrauma care services, improving the effectiveness of interventions by the emergency services, reducing the rescue time and therefore minimizing the consequences involved and the number of victims. This could often mean saving lives. In the different simulated rescue operations, the rescue time has been reduced an average of 14%.

Mountaineering accidents in the European Alps: have the numbers increased in recent years?

PubMed

Lischke, V; Byhahn, C; Westphal, K; Kessler, P

2001-01-01

Media reports convey the impression that the incidence of fatal accidents in the European Alps has increased. Because more specific data are lacking, we analyzed available data from the mountain rescue services in Germany, Austria, southern Tirol, Zermatt/Switzerland, and Chamonix/France from 1987 until 1997. Information was gathered from the annual reports of the Austrian Mountain Rescue Service, the Swiss Alpine Club Rescue Station in Zermatt, the Mountain Rescue Service of the Southern Tirol Alpine Club, the Mountain Rescue Service of the Bavarian Red Cross, and the Department of Mountain Medicine and Traumatology from the Hospital in Chamonix. Although the total number of rescue missions and injured alpinists increased significantly during the period, the number of fatalities retrieved during such rescue missions showed no significant increase. Even taking into account the varying definitions of "mountain accident" used in these countries, available data from the analyzed areas of the European Alps do not demonstrate a drastic increase in the number of fatalities. In the future, data concerning mountain accidents in the European Alps should be monitored according to standard definitions and stored by the International Commission for Alpine Rescue.

Genomic deletions created upon LINE-1 retrotransposition.

PubMed

Gilbert, Nicolas; Lutz-Prigge, Sheila; Moran, John V

2002-08-09

LINE-1 (L1) retrotransposition continues to impact the human genome, yet little is known about how L1 integrates into DNA. Here, we developed a plasmid-based rescue system and have used it to recover 37 new L1 retrotransposition events from cultured human cells. Sequencing of the insertions revealed the usual L1 structural hallmarks; however, in four instances, retrotransposition generated large target site deletions. Remarkably, three of those resulted in the formation of chimeric L1s, containing the 5' end of an endogenous L1 fused precisely to our engineered L1. Thus, our data demonstrate multiple pathways for L1 integration in cultured cells, and show that L1 is not simply an insertional mutagen, but that its retrotransposition can result in significant deletions of genomic sequence.

KSC-07pd3079

NASA Image and Video Library

2007-11-02

KENNEDY SPACE CENTER, FLA. -- During a rehearsal, Center Director Bill Parsons helps introduce the World Space Expo Aerial Salute at NASA's Kennedy Space Center. Commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond, the expo will showcase various panels, presentations and educational programs, as well as the air show. Participating in the air show are the U.S. Air Force Thunderbirds, U.S. Air Force F-22 Raptor, U.S. Navy F-18 Super Hornet, U.S. Air Force F-15 Eagle, the P-51 Mustang Heritage Flight, and the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery. The U.S. Army Golden Knights will also demonstrate precision skydiving. Photo credit: NASA/George Shelton

TGFbeta receptor saxophone non-autonomously regulates germline proliferation in a Smox/dSmad2-dependent manner in Drosophila testis.

PubMed

Li, Chun-Yan; Guo, Zheng; Wang, Zhaohui

2007-09-01

Elucidating the regulatory mechanism of cell proliferation is central to the understanding of cancer development or organ size control. Drosophila spermatogenesis provides an excellent model to study cell proliferation since the germline cells mitotically amplify in a precise manner. However, the underlying molecular mechanism remains elusive. Germ cells derived from each gonialblast develop synchronously as one unit encapsulated by two somatic support cells (called cyst cells). Components of TGFbeta pathway have previously been found to restrict germ cell proliferation via their functions in cyst cells. Here we report that saxophone (sax), a TGFbeta type I receptor, is required in somatic cells to prevent the mitotically dividing spermatogonia from over-amplifying. Using various approaches, we demonstrate that Mad (Mothers against Dpp), a receptor-Smad usually associated with Sax-mediated TGFbeta/BMP signaling, is dispensable in this process. Instead, Smox (Smad on X, Drosophila Smad2), the other receptor-Smad formerly characterized in TGFbeta/activin signaling, is necessary for the precise mitotic divisions of spermatogonia. Furthermore, over-expressing Smox in cyst cells can partially rescue the proliferation phenotype induced by sax mutation. We propose that Smox acts downstream of Sax to prevent spermatogonial over-proliferation in Drosophila.

Optogenetic approaches to evaluate striatal function in animal models of Parkinson disease.

PubMed

Parker, Krystal L; Kim, Youngcho; Alberico, Stephanie L; Emmons, Eric B; Narayanan, Nandakumar S

2016-03-01

Optogenetics refers to the ability to control cells that have been genetically modified to express light-sensitive ion channels. The introduction of optogenetic approaches has facilitated the dissection of neural circuits. Optogenetics allows for the precise stimulation and inhibition of specific sets of neurons and their projections with fine temporal specificity. These techniques are ideally suited to investigating neural circuitry underlying motor and cognitive dysfunction in animal models of human disease. Here, we focus on how optogenetics has been used over the last decade to probe striatal circuits that are involved in Parkinson disease, a neurodegenerative condition involving motor and cognitive abnormalities resulting from degeneration of midbrain dopaminergic neurons. The precise mechanisms underlying the striatal contribution to both cognitive and motor dysfunction in Parkinson disease are unknown. Although optogenetic approaches are somewhat removed from clinical use, insight from these studies can help identify novel therapeutic targets and may inspire new treatments for Parkinson disease. Elucidating how neuronal and behavioral functions are influenced and potentially rescued by optogenetic manipulation in animal models could prove to be translatable to humans. These insights can be used to guide future brain-stimulation approaches for motor and cognitive abnormalities in Parkinson disease and other neuropsychiatric diseases.

Cellular Reprogramming, Genome Editing, and Alternative CRISPR Cas9 Technologies for Precise Gene Therapy of Duchenne Muscular Dystrophy

PubMed Central

Xu, Huaigeng

2017-01-01

In the past decade, the development of two innovative technologies, namely, induced pluripotent stem cells (iPSCs) and the CRISPR Cas9 system, has enabled researchers to model diseases derived from patient cells and precisely edit DNA sequences of interest, respectively. In particular, Duchenne muscular dystrophy (DMD) has been an exemplary monogenic disease model for combining these technologies to demonstrate that genome editing can correct genetic mutations in DMD patient-derived iPSCs. DMD is an X-linked genetic disorder caused by mutations that disrupt the open reading frame of the dystrophin gene, which plays a critical role in stabilizing muscle cells during contraction and relaxation. The CRISPR Cas9 system has been shown to be capable of targeting the dystrophin gene and rescuing its expression in in vitro patient-derived iPSCs and in vivo DMD mouse models. In this review, we highlight recent advances made using the CRISPR Cas9 system to correct genetic mutations and discuss how emerging CRISPR technologies and iPSCs in a combined platform can play a role in bringing a therapy for DMD closer to the clinic. PMID:28607562

KSC-07pd3150

NASA Image and Video Library

2007-11-06

KENNEDY SPACE CENTER, FLA. -- The air crew for the U.S. Air Force Thunderbirds Demonstration Squadron show their precision formation as the planes prepare to take off from NASA's Kennedy Space Center. The squadron performed for the World Space Expos' aerial salute along with other aircraft that included the U.S. Navy F-18 Super Hornets, U.S. Air Force F-22 Raptor, U.S. Air Force F-15 Eagle, P-51 Mustang Heritage Flight, the U.S. Air Force 920th Rescue wing, which was responsible for Mercury and Gemini capsule recovery, and the U.S. Army Golden Knights precision skydiving team. The World Space Expo held Nov. 1-4 was an event commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond. The expo showcased various panels, presentations and educational programs. It also was a part of NASA's 50th anniversary celebrations, highlighting the 45th Anniversary of the Mercury Program celebration featuring original NASA astronauts John Glenn and Scott Carpenter and the Pioneering Women of Aerospace forum featuring Eileen Collins and other prominent female space veterans. The agency was founded Oct. 1, 1958. Photo credit: NASA/Jim Grossmann

KSC-07pd3151

NASA Image and Video Library

2007-11-06

KENNEDY SPACE CENTER, FLA. -- The air crew for the U.S. Air Force Thunderbirds Demonstration Squadron show their precision formation as the planes prepare to take off from NASA's Kennedy Space Center. The squadron performed for the World Space Expos' aerial salute along with other aircraft that included the U.S. Navy F-18 Super Hornets, U.S. Air Force F-22 Raptor, U.S. Air Force F-15 Eagle, P-51 Mustang Heritage Flight, the U.S. Air Force 920th Rescue wing, which was responsible for Mercury and Gemini capsule recovery, and the U.S. Army Golden Knights precision skydiving team. The World Space Expo held Nov. 1-4 was an event commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond. The expo showcased various panels, presentations and educational programs. It also was a part of NASA's 50th anniversary celebrations, highlighting the 45th Anniversary of the Mercury Program celebration featuring original NASA astronauts John Glenn and Scott Carpenter and the Pioneering Women of Aerospace forum featuring Eileen Collins and other prominent female space veterans. The agency was founded Oct. 1, 1958. Photo credit: NASA/Jim Grossmann

KSC-07pd3148

NASA Image and Video Library

2007-11-06

KENNEDY SPACE CENTER, FLA. -- The air crew for the U.S. Air Force Thunderbirds Demonstration Squadron show their precision formation as the pilots get ready to take off from NASA's Kennedy Space Center. The squadron performed for the World Space Expos' aerial salute along with other aircraft that included the U.S. Navy F-18 Super Hornets, U.S. Air Force F-22 Raptor, U.S. Air Force F-15 Eagle, P-51 Mustang Heritage Flight, the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery, and the U.S. Army Golden Knights precision skydiving team. The World Space Expo held Nov. 1-4 was an event commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond. The expo showcased various panels, presentations and educational programs. It also was a part of NASA's 50th anniversary celebrations, highlighting the 45th Anniversary of the Mercury Program celebration featuring original NASA astronauts John Glenn and Scott Carpenter and the Pioneering Women of Aerospace forum featuring Eileen Collins and other prominent female space veterans. The agency was founded Oct. 1, 1958. Photo credit: NASA/Jim Grossmann

KSC-07pd3149

NASA Image and Video Library

2007-11-06

KENNEDY SPACE CENTER, FLA. -- The air crew for the U.S. Air Force Thunderbirds Demonstration Squadron show their precision formation as the planes prepare to take off from NASA's Kennedy Space Center. The squadron performed for the World Space Expos' aerial salute along with other aircraft that included the U.S. Navy F-18 Super Hornets, U.S. Air Force F-22 Raptor, U.S. Air Force F-15 Eagle, P-51 Mustang Heritage Flight, the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery, and the U.S. Army Golden Knights precision skydiving team. The World Space Expo held Nov. 1-4 was an event commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond. The expo showcased various panels, presentations and educational programs. It also was a part of NASA's 50th anniversary celebrations, highlighting the 45th Anniversary of the Mercury Program celebration featuring original NASA astronauts John Glenn and Scott Carpenter and the Pioneering Women of Aerospace forum featuring Eileen Collins and other prominent female space veterans. The agency was founded Oct. 1, 1958. Photo credit: NASA/Jim Grossmann

The problem with rescue medicine.

PubMed

Jecker, Nancy S

2013-02-01

Is there a rational and ethical basis for efforts to rescue individuals in dire straits? When does rescue have ethical support, and when does it reflect an irrational impulse? This paper defines a Rule of Rescue and shows its intuitive appeal. It then proceeds to argue that this rule lacks support from standard principles of justice and from ethical principles more broadly, and should be rejected in many situations. I distinguish between agent-relative and agent-neutral reasons, and argue that the Rule of Rescue qualifies only in a narrow range of cases where agent-relative considerations apply. I conclude that it would be wise to set aside the Rule of Rescue in many cases, especially those involving public policies, where it has only weak normative justification. The broader implications of this analysis are noted.

46 CFR 160.156-9 - Preapproval review.

Code of Federal Regulations, 2012 CFR

2012-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-9... fast rescue boats; (7) Hull and canopy construction drawings, including particulars of joints, welds...

46 CFR 160.156-9 - Preapproval review.

Code of Federal Regulations, 2014 CFR

2014-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-9... fast rescue boats; (7) Hull and canopy construction drawings, including particulars of joints, welds...

46 CFR 160.156-9 - Preapproval review.

Code of Federal Regulations, 2013 CFR

2013-10-01

...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-9... fast rescue boats; (7) Hull and canopy construction drawings, including particulars of joints, welds...

A retrospective study of the performance of video laryngoscopy in an obstetric unit.

PubMed

Aziz, Michael F; Kim, Diana; Mako, Jeffrey; Hand, Karen; Brambrink, Ansgar M

2012-10-01

We evaluated the performance of tracheal intubation using video laryngoscopy in an obstetric unit. We analyzed airway management details during a 3-year period, and observed 180 intubations. All cases were managed with direct or video laryngoscopy. Direct laryngoscopy resulted in 157 out of 163 (95% confidence interval [CI], 92%-99%) first attempt successful intubations and failed once. Video laryngoscopy resulted in 18 of 18 (95% CI, 81%-100%) successful intubations on first attempt. The failed direct laryngoscopy was rescued with video laryngoscopy. The patients managed with video laryngoscopy frequently required urgent or emergency surgery and had predictors of difficult direct laryngoscopy in 16 of 18 cases. Video laryngoscopy may be a useful adjunct for obstetric airway management, and its role in this difficult airway scenario should be further studied.

Knockdown of Triglyceride Synthesis Does Not Enhance Palmitate Lipotoxicity or Prevent Oleate-Mediated Rescue in Rat Hepatocytes

PubMed Central

Leamy, Alexandra K.; Hasenour, Clinton M.; Egnatchik, Robert A.; Trenary, Irina A.; Yao, Conghui; Patti, Gary J.; Shiota, Masakazu; Young, Jamey D.

2016-01-01

Experiments in a variety of cell types, including hepatocytes, consistently demonstrate the acutely lipotoxic effects of saturated fatty acids, such as palmitate (PA), but not unsaturated fatty acids, such as oleate (OA). PA+OA co-treatment fully prevents PA lipotoxicity through mechanisms that are not well defined but which have been previously attributed to more efficient esterification and sequestration of PA into triglycerides (TGs) when OA is abundant. However, this hypothesis has never been directly tested by experimentally modulating the relative partitioning of PA/OA between TGs and other lipid fates in hepatocytes. In this study, we found that addition of OA to PA-treated hepatocytes enhanced TG synthesis, reduced total PA uptake and PA lipid incorporation, decreased phospholipid saturation and rescued PA-induced ER stress and lipoapotosis. Knockdown of diacylglycerol acyltransferase (DGAT), the rate-limiting step in TG synthesis, significantly reduced TG accumulation without impairing OAmediated rescue of PA lipotoxicity. In both wild-type and DGAT-knockdown hepatocytes, OA cotreatment significantly reduced PA lipid incorporation and overall phospholipid saturation compared to PA-treated hepatocytes. These data indicate that OA’s protective effects do not require increased conversion of PA into inert TGs, but instead may be due to OA’s ability to compete against PA for cellular uptake and/or esterification and, thereby, normalize the composition of cellular lipids in the presence of a toxic PA load. PMID:27249207

Rescue of Targeted Regions of Mammalian Chromosomes by in Vivo Recombination in Yeast

PubMed Central

Kouprina, Natalya; Kawamoto, Kensaku; Barrett, J. Carl; Larionov, Vladimir; Koi, Minoru

1998-01-01

In contrast to other animal cell lines, the chicken pre-B cell lymphoma line, DT40, exhibits a high level of homologous recombination, which can be exploited to generate site-specific alterations in defined target genes or regions. In addition, the ability to generate human/chicken monochromosomal hybrids in the DT40 cell line opens a way for specific targeting of human genes. Here we describe a new strategy for direct isolation of a human chromosomal region that is based on targeting of the chromosome with a vector containing a yeast selectable marker, centromere, and an ARS element. This procedure allows rescue of the targeted region by transfection of total genomic DNA into yeast spheroplasts. Selection for the yeast marker results in isolation of chromosome sequences in the form of large circular yeast artificial chromosomes (YACs) up to 170 kb in size containing the targeted region. These YACs are generated by homologous recombination in yeast between common repeated sequences in the targeted chromosomal fragment. Alternatively, the targeted region can be rescued as a linear YACs when a YAC fragmentation vector is included in the yeast transformation mixture. Because the entire isolation procedure of the chromosomal region, once a target insertion is obtained, can be accomplished in ∼1 week, the new method greatly expands the utility of the homologous recombinationproficient DT40 chicken cell system. PMID:9647640

T-bet Down-Modulation in Tolerized Th1 Effector CD4 Cells Confers a TCR-Distal Signaling Defect That Selectively Impairs IFN-γ Expression1

PubMed Central

Long, Meixiao; Slaiby, Aaron M.; Hagymasi, Adam T.; Mihalyo, Marianne A.; Lichtler, Alexander C.; Reiner, Steven L.; Adler, Adam J.

2010-01-01

When Th1 effector CD4 cells encounter tolerizing Ag in vivo, their capacity to express the effector cytokines IFN-γ and TNF-α is lost more rapidly than noneffector functions such as IL-2 production and proliferation. To localize the relevant intracellular signaling defects, cytokine expression was compared following restimulation with Ag vs agents that bypass TCR-proximal signaling. IFN-γ and TNF-α expression were both partially rescued when TCR-proximal signaling was bypassed, indicating that both TCR-proximal and -distal signaling defects impair the expression of these two effector cytokines. In contrast, bypassing TCR-proximal signaling fully rescued IL-2 expression. T-bet, a transcription and chromatin remodeling factor that is required to direct the differentiation of naive CD4 cells into IFN-γ -expressing Th1 effectors, was partially down-modulated in tolerized Th1 effectors. Enforcing T-bet expression during tolerization selectively rescued the ability to express IFN-γ, but not TNF-α. Conversely, expression of a dominant-negative T-bet in Th1 effectors selectively impaired the ability to express IFN-γ, but not TNF-α. Analysis of histone acetylation at the IFN-γ promoter further suggested that down-modulation of T-bet expression during Th1 effector CD4 cell tolerization does not impair IFN-γ expression potential through alterations in chromatin structure. PMID:16393991

Investigating Lushan Earthquake Victims' Individual Behavior Response and Rescue Organization.

PubMed

Kang, Peng; Lv, Yipeng; Deng, Qiangyu; Liu, Yuan; Zhang, Yi; Liu, Xu; Zhang, Lulu

2017-12-11

Research concerning the impact of earthquake victims' individual behavior and its association with earthquake-related injuries is lacking. This study examined this relationship along with effectiveness of earthquake rescue measures. The six most severely destroyed townships during the Lushan earthquake were examined; 28 villages and three earthquake victims' settlement camp areas were selected as research areas. Inclusion criteria comprised living in Lushan county for a longtime, living in Lushan county during the 2013 Lushan earthquake, and having one's home destroyed. Earthquake victims with an intellectual disability or communication problems were excluded. The earthquake victims (N (number) = 5165, male = 2396) completed a questionnaire (response rate: 94.7%). Among them, 209 were injured (5.61%). Teachers (p < 0.0001, OR (odds ratios) = 3.33) and medical staff (p = 0.001, OR = 4.35) were more vulnerable to the earthquake than were farmers. Individual behavior was directly related to injuries, such as the first reaction after earthquake and fear. There is an obvious connection between earthquake-related injury and individual behavior characteristics. It is strongly suggested that victims receive mental health support from medical practitioners and the government to minimize negative effects. The initial reaction after an earthquake also played a vital role in victims' trauma; therefore, earthquake-related experience and education may prevent injuries. Self-aid and mutual help played key roles in emergency, medical rescue efforts.

Rescuing--a universal phenomenon.

PubMed

Little, John

2014-12-01

Rescuing, where the person is delivered from the immediacy of their conundrum by another, complicates management. The object of this paper is to understand the difficulty in relinquishing the rescuing role. Rescuing is a universal phenomenon in parenting, teaching and therapy that has developed over time through a variety of interwoven social, economic, psychological and clinical variables. © The Royal Australian and New Zealand College of Psychiatrists 2014.

Enhanced Rescue Lift Capability

NASA Technical Reports Server (NTRS)

Young, Larry A.

2007-01-01

The evolving and ever-increasing demands of emergency response and disaster relief support provided by rotorcraft dictate, among other things, the development of enhanced rescue lift capability for these platforms. This preliminary analysis is first-order in nature but provides considerable insight into some of the challenges inherent in trying to effect rescue using a unique form of robotic rescue device deployed and operated from rotary-wing aerial platforms.

33 CFR 149.304 - What type and how many survival craft and rescue boats must a manned deepwater port have?

Code of Federal Regulations, 2012 CFR

2012-07-01

... craft and rescue boats must a manned deepwater port have? 149.304 Section 149.304 Navigation and... What type and how many survival craft and rescue boats must a manned deepwater port have? (a) Except as... of survival craft and the number of rescue boats indicated for the deepwater port in paragraphs (a)(1...

33 CFR 149.304 - What type and how many survival craft and rescue boats must a manned deepwater port have?

Code of Federal Regulations, 2013 CFR

2013-07-01

... craft and rescue boats must a manned deepwater port have? 149.304 Section 149.304 Navigation and... What type and how many survival craft and rescue boats must a manned deepwater port have? (a) Except as... survival craft and the number of rescue boats indicated for the deepwater port in paragraphs (a)(1) through...

33 CFR 149.304 - What type and how many survival craft and rescue boats must a manned deepwater port have?

Code of Federal Regulations, 2014 CFR

2014-07-01

... craft and rescue boats must a manned deepwater port have? 149.304 Section 149.304 Navigation and... What type and how many survival craft and rescue boats must a manned deepwater port have? (a) Except as... survival craft and the number of rescue boats indicated for the deepwater port in paragraphs (a)(1) through...

33 CFR 149.304 - What type and how many survival craft and rescue boats must a manned deepwater port have?

Code of Federal Regulations, 2011 CFR

2011-07-01

... craft and rescue boats must a manned deepwater port have? 149.304 Section 149.304 Navigation and... What type and how many survival craft and rescue boats must a manned deepwater port have? (a) Except as... of survival craft and the number of rescue boats indicated for the deepwater port in paragraphs (a)(1...

D1 Receptor Activation in the Mushroom Bodies Rescues Sleep Loss Induced Learning Impairments in Drosophila

PubMed Central

Seugnet, Laurent; Suzuki, Yasuko; Vine, Lucy; Gottschalk, Laura; Shaw, Paul J

2008-01-01

Background Extended wakefulness disrupts acquisition of short term memories in mammals. However, the underlying molecular mechanisms triggered by extended waking and restored by sleep are unknown. Moreover, the neuronal circuits that depend on sleep for optimal learning remain unidentified. Results Learning was evaluated using Aversive Phototaxic Suppression (APS). In this task, flies learn to avoid light that is paired with an aversive stimulus (quinine /humidity). We demonstrate extensive homology in sleep deprivation induced learning impairment between flies and humans. Both 6 h and 12 h of sleep deprivation are sufficient to impair learning in Canton-S (Cs) flies. Moreover, learning is impaired at the end of the normal waking-day in direct correlation with time spent awake. Mechanistic studies indicate that this task requires intact mushroom bodies (MBs) and requires the Dopamine D1-like receptor (dDA1). Importantly, sleep deprivation induced learning impairments could be rescued by targeted gene expression of the dDA1 receptor to the MBs. Conclusion These data provide direct evidence that extended wakefulness disrupts learning in Drosophila. These results demonstrate that it is possible to prevent the effects of sleep deprivation by targeting a single neuronal structure and identify cellular and molecular targets adversely affected by extended waking in a genetically tractable model organism. PMID:18674913

Search and rescue in Alaska's national parks.

PubMed

Heggie, Travis W

2008-11-01

Recreational travel to wilderness destinations such as national parks is increasing. The inherent risks present in such destinations can result in injury and illness, have a significant impact on local medical resources, and end with expensive search and rescue operations. In order to increase our understanding of the activities and situations which lead to wilderness search and rescue incidents, this study examines search and rescue operations from National Park Service units in Alaska. A retrospective review of all search and rescue incident reports filed by National Park Service units in Alaska during 2002. During 2002 there were 25 reported search and rescue incidents involving 38 individuals. The majority of incidents (19 of 25) occurred at Denali National Park and Preserve. Thirteen fatalities were reported in six incidents, nine incidents involved traumatic injuries, eight involved illnesses, and two involved both injuries and an illness. Mountain climbing (20) and hiking (8) were the most common subject activities at the time search and rescue assistance was required. Climbing solo (4), uneven and wet terrain (4), falls into crevasses (3), and a lack of experience or ability (3) were the factors most commonly contributing to search and rescue incidents. Nineteen helicopters were utilized in 15 operations and fixed-wing aircraft were utilized in seven operations. Males accounted for 33 of the 38 individuals involved in all search and rescue incidents and United States citizens accounted for 74% of the individuals involved. The mountain environment higher than 4500m was the most common search and rescue environment (11). The average cost was USD $6253. Search and rescue operations in Alaska can be expensive and end with severe health consequences. Preventive education efforts at park visitor centers and at the lower and upper base camps on Mt. McKinley should be continued. In addition, pre-departure travel education efforts via the internet should be expanded for all park units and match the detail provided on the Denali National Park and Preserve website.

Effect of rescue breathing by lay rescuers for out-of-hospital cardiac arrest caused by respiratory disease: a nationwide, population-based, propensity score-matched study.

PubMed

Fukuda, Tatsuma; Ohashi-Fukuda, Naoko; Kondo, Yutaka; Sera, Toshiki; Yahagi, Naoki

2017-06-01

The importance of respiratory care in cardiopulmonary resuscitation may vary depending on the cause of cardiac arrest. No previous study has investigated the effects of rescue breathing performed by a lay rescuer on the outcomes of patients with out-of-hospital cardiac arrest (OHCA) caused by intrinsic respiratory diseases. The aim of this study was to investigate whether rescue breathing performed by a lay rescuer is associated with outcomes after respiratory disease-related OHCA. In a nationwide, population-based, propensity score-matched study in Japan, among adult patients with OHCA caused by respiratory disease who received bystander cardiopulmonary resuscitation from January 1, 2005 to December 31, 2010, we compared patients with rescue breathing to those without rescue breathing. The primary outcome was neurologically favorable survival 1 month after OHCA. Of the eligible 14,781 patients, 4970 received rescue breathing from a lay rescuer and 9811 did not receive rescue breathing. In a propensity score-matched cohort (4897 vs. 4897 patients), the neurologically favorable survival rate was similar between patients with and without rescue breathing from a lay rescuer [0.9 vs. 0.7 %; OR 1.23 (95 % CI 0.79-1.93)]. Additionally, in subgroup analyses, rescue breathing was not associated with neurological outcome regardless of the type of rescuer [family member: adjusted OR 0.83 (95 % CI 0.39-1.70); or non-family member: adjusted OR 1.91 (95 % CI 0.79-5.35)]. Even among patients with OHCA caused by respiratory disease, rescue breathing performed by a lay rescuer was not associated with neurological outcomes, regardless of the type of lay rescuer.

30 CFR 49.9 - Mine emergency notification plan.

Code of Federal Regulations, 2011 CFR

2011-07-01

... TRAINING MINE RESCUE TEAMS § 49.9 Mine emergency notification plan. (a) Each underground mine shall have a mine rescue notification plan outlining the procedures to follow in notifying the mine rescue teams...

30 CFR 49.9 - Mine emergency notification plan.

Code of Federal Regulations, 2010 CFR

2010-07-01

... TRAINING MINE RESCUE TEAMS § 49.9 Mine emergency notification plan. (a) Each underground mine shall have a mine rescue notification plan outlining the procedures to follow in notifying the mine rescue teams...

46 CFR 160.156-1 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... APPROVAL LIFESAVING EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-1 Scope. This subpart... fast rescue boat, complying with SOLAS and the IMO LSA Code, for use on waters other than protected...

46 CFR 160.156-1 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... APPROVAL LIFESAVING EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-1 Scope. This subpart... fast rescue boat, complying with SOLAS and the IMO LSA Code, for use on waters other than protected...

46 CFR 160.156-1 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... APPROVAL LIFESAVING EQUIPMENT Rescue Boats and Fast Rescue Boats (SOLAS) § 160.156-1 Scope. This subpart... fast rescue boat, complying with SOLAS and the IMO LSA Code, for use on waters other than protected...

Field studies of safety security rescue technologies through training and response activities

NASA Astrophysics Data System (ADS)

Murphy, Robin R.; Stover, Sam

2006-05-01

This paper describes the field-oriented philosophy of the Institute for Safety Security Rescue Technology (iSSRT) and summarizes the activities and lessons learned during calendar year 2005 of its two centers: the Center for Robot-Assisted Search and Rescue and the NSF Safety Security Rescue industry/university cooperative research center. In 2005, iSSRT participated in four responses (La Conchita, CA, Mudslides, Hurricane Dennis, Hurricane Katrina, Hurricane Wilma) and conducted three field experiments (NJTF-1, Camp Hurricane, Richmond, MO). The lessons learned covered mobility, operator control units, wireless communications, and general reliability. The work has collectively identified six emerging issues for future work. Based on these studies, a 10-hour, 1 continuing education unit credit course on rescue robotics has been created and is available. Rescue robots and sensors are available for loan upon request.

The German Sea Rescue Service (SAR).

PubMed

2002-01-01

The German Sea Rescue Service (GSRS) history, organisation and operations are presented. The institution was founded in 1865 to provide sea rescue services for the German coasts in the North Sea and in the Baltic Sea. Its fleet counts 61 vessels based in 54 stations of the service. In 2001, the rescue crafts were called for assistance 2428 times and 207 lives were saved. The service is supported by voluntary donations.

Running on Empty: The Development of Helicopter Aerial Refueling and Implications for Future USAF Combat Rescue Capabilities

DTIC Science & Technology

1997-03-01

Haiti, Somalia, Liberia, and Bosnia. The future appears very busy for Air Force rescue units as well. According to ?Strategic Assessment 1996?Instruments...13 COMTEMPORARY ROLE OF AIR REFUELING IN US AIR FORCE SEARCH AND RESCUE...places such as Haiti, Somalia, Liberia, and Bosnia. The future appears very busy for Air Force rescue units as well. According to “Strategic Assessment

Determining the need for rescue intracytoplasmic sperm injection in partial fertilisation failure during a conventional IVF cycle.

PubMed

Cao, S; Wu, X; Zhao, C; Zhou, L; Zhang, J; Ling, X

2016-12-01

To explore the need for rescue intracytoplasmic sperm injection (ICSI) in cases of partial fertilisation failure during a conventional in vitro fertilisation cycle, rescue ICSI was performed for cycles with a fertilisation rate of <50%. The data were divided into three groups based on the fertilisation rate: group 1 (0%), group 2 (<25%) and group 3 (>25%). The impact of rescue ICSI on each group was then analysed in terms of ovum fertilisation, embryo development, embryo utilisation and selection of embryos for transfer. Rescue ICSI was performed on 1831 unfertilised oocytes from 313 cycles. The fertilisation rates for group 1, group 2 and group 3 were 74.66, 68.35 and 65.46%, and the rate of polyploidy in the three groups was 8.55, 11.33, and 14.47%. The percentage of embryos that can be transferred from rescue ICSI for group 2 was 38.33%, and this value was higher than those of the other two groups. It is concluded that rescue ICSI is not recommended for patients with an IVF rate of >25% as the procedure is associated with a greater risk and low returns. However, it is feasible to perform rescue ICSI for patients with IVF rates of <25%. © 2016 Blackwell Verlag GmbH.

'Don't save her'- Sigmund Freud meets Project Pat: the rescue motif in hip-hop.

PubMed

Fulmer, Richard H

2008-08-01

Freud originally explicated the dynamics of rescue wishes by describing men who fell in love with prostitutes. He saw this attempt at attachment as driven by the man's wish to repay his parents for giving him life. Many subsequent writers shift this emphasis, seeing rescue wishes as motivated by aggressive oedipal competition with the father. This article highlights the attachment aspects of Freud's original conception and traces how writers in the last three decades use the family romance rather than Oedipus as a model to view rescue wishes as having a more tender aspect. Rescue wishes are especially characteristic of the developmental stage of young adulthood. They at once attempt to repay the parental debt the young adult feels and serve as practice for the vicissitudes of the couple bond and the benign sacrifices of parenthood. Popular culture contains many vivid examples of Freud 's original description of the rescue-motif. A detailed examination of the rap song, Don't save her, demonstrates all the elements of Freud's original conception and the interpersonal risks of the rescue relationship. Three additional rap songs by other artists are briefly analyzed to show the extensive occurrence of the wish to rescue and its psychic and interpersonal dangers.

30 CFR 49.19 - Mine emergency notification plan.

Code of Federal Regulations, 2012 CFR

2012-07-01

... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Coal Mines § 49.19 Mine emergency notification... follow in notifying the mine rescue teams when there is an emergency that requires their services. (b) A...

30 CFR 49.6 - Equipment and maintenance requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... TRAINING MINE RESCUE TEAMS Mine Rescue Teams for Underground Metal and Nonmetal Mines § 49.6 Equipment and... sufficient to sustain each team for eight hours while using the breathing apparatus during rescue operations...