Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed rats.

PubMed

Li, E; Deng, Haifeng; Wang, Bo; Fu, Wan; You, Yong; Tian, Shaowen

2016-03-01

Apelin is the endogenous ligand for the G-protein-coupled receptor (APJ). The localization of APJ in limbic structures suggests a potential role for apelin in emotional processes. However, the role of apelin in the regulation of stress-induced responses such as depression and memory impairment is largely unknown. In the present study, we evaluated the role of apelin-13 in the regulation of stress-induced depression and memory impairment in rats. We report that repeated intracerebroventricular injections of apelin-13 reversed behavioral despair (immobility) in the forced swim (FS) test, a model widely used for the selection of new antidepressant agents. Apelin-13 also reversed behavioral deficits (escape failure) in the learned helplessness test. The magnitude of the antiimmobility and anti-escape failure effects of apelin-13 was comparable to that of imipramine, a classic antidepressant used as a positive control. Rats exposed to FS stress showed memory performance impairment in the novel object recognition test, and this impairment was improved by apelin-13 treatment. Apelin-13 did not affect recognition memory performance in non-stressed rats. Furthermore, the pretreatment of LY294002 (PI3K inhibitors) or PD98059 (ERK1/2 inhibitor) blocked apelin-13-mediated activities in FS-stressed rats. These findings suggest that apelin-13 exerts antidepressant-like and recognition memory improving activities through activating PI3K and ERK1/2 signaling pathways in stressed rats. Copyright © 2016 Elsevier B.V. and ECNP. All rights reserved.

High-CO 2 Requirement as a Mechanism for the Containment of Genetically Modified Cyanobacteria

DOE PAGES

Clark, Ryan L.; Gordon, Gina C.; Bennett, Nathaniel R.; ...

2018-01-10

As researchers engineer cyanobacteria for biotechnological applications, we must consider potential environmental release of these organisms. Previous theoretical work has considered cyanobacterial containment through elimination of the CO 2-concentrating mechanism (CCM) to impose a high-CO 2 requirement (HCR), which could be provided in the cultivation environment but not in the surroundings. In this work, we experimentally implemented an HCR containment mechanism in Synechococcus sp. strain PCC7002 (PCC7002) through deletion of carboxysome shell proteins and showed that this mechanism contained cyanobacteria in a 5% CO 2 environment. We considered escape through horizontal gene transfer (HGT) and reduced the risk of HGTmore » escape by deleting competence genes. We showed that the HCR containment mechanism did not negatively impact the performance of a strain of PCC7002 engineered for L-lactate production. In conclusion, we showed through coculture experiments of HCR strains with ccm-containing strains that this HCR mechanism reduced the frequency of escape below the NIH recommended limit for recombinant organisms of one escape event in 10 8 CFU.« less

High-CO 2 Requirement as a Mechanism for the Containment of Genetically Modified Cyanobacteria

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clark, Ryan L.; Gordon, Gina C.; Bennett, Nathaniel R.

As researchers engineer cyanobacteria for biotechnological applications, we must consider potential environmental release of these organisms. Previous theoretical work has considered cyanobacterial containment through elimination of the CO 2-concentrating mechanism (CCM) to impose a high-CO 2 requirement (HCR), which could be provided in the cultivation environment but not in the surroundings. In this work, we experimentally implemented an HCR containment mechanism in Synechococcus sp. strain PCC7002 (PCC7002) through deletion of carboxysome shell proteins and showed that this mechanism contained cyanobacteria in a 5% CO 2 environment. We considered escape through horizontal gene transfer (HGT) and reduced the risk of HGTmore » escape by deleting competence genes. We showed that the HCR containment mechanism did not negatively impact the performance of a strain of PCC7002 engineered for L-lactate production. In conclusion, we showed through coculture experiments of HCR strains with ccm-containing strains that this HCR mechanism reduced the frequency of escape below the NIH recommended limit for recombinant organisms of one escape event in 10 8 CFU.« less

A Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite.

PubMed

Hirota, Ryuichi; Abe, Kenji; Katsuura, Zen-Ichiro; Noguchi, Reiji; Moribe, Shigeaki; Motomura, Kei; Ishida, Takenori; Alexandrov, Maxym; Funabashi, Hisakage; Ikeda, Takeshi; Kuroda, Akio

2017-03-20

There is a growing demand to develop biocontainment strategies that prevent unintended proliferation of genetically modified organisms in the open environment. We found that the hypophosphite (H 3 PO 2 , HPt) transporter HtxBCDE from Pseudomonas stutzeri WM88 was also capable of transporting phosphite (H 3 PO 3 , Pt) but not phosphate (H 3 PO 4 , Pi), suggesting the potential for engineering a Pt/HPt-dependent bacterial strain as a biocontainment strategy. We disrupted all Pi and organic Pi transporters in an Escherichia coli strain expressing HtxABCDE and a Pt dehydrogenase, leaving Pt/HPt uptake and oxidation as the only means to obtain Pi. Challenge on non-permissive growth medium revealed that no escape mutants appeared for at least 21 days with a detection limit of 1.94 × 10 -13 per colony forming unit. This represents, to the best of our knowledge, the lowest escape frequency among reported strategies. Since Pt/HPt are ecologically rare and not available in amounts sufficient for the growth of the Pt/HPt-dependent bacteria, this strategy offers a reliable and practical method for biocontainment.

The Establishment of Genetically Engineered Canola Populations in the U.S.

PubMed Central

Schafer, Meredith G.; Ross, Andrew A.; Londo, Jason P.; Burdick, Connie A.; Lee, E. Henry; Travers, Steven E.; Van de Water, Peter K.; Sagers, Cynthia L.

2011-01-01

Concerns regarding the commercial release of genetically engineered (GE) crops include naturalization, introgression to sexually compatible relatives and the transfer of beneficial traits to native and weedy species through hybridization. To date there have been few documented reports of escape leading some researchers to question the environmental risks of biotech products. In this study we conducted a systematic roadside survey of canola (Brassica napus) populations growing outside of cultivation in North Dakota, USA, the dominant canola growing region in the U.S. We document the presence of two escaped, transgenic genotypes, as well as non-GE canola, and provide evidence of novel combinations of transgenic forms in the wild. Our results demonstrate that feral populations are large and widespread. Moreover, flowering times of escaped populations, as well as the fertile condition of the majority of collections suggest that these populations are established and persistent outside of cultivation. PMID:21998689

Waiting for O2

NASA Astrophysics Data System (ADS)

Zahnle, K. J.; Catling, D. C.; Claire, M.

2011-12-01

Oxygenic photosynthesis appears to be a necessary condition for the creation of an oxygen-rich atmosphere like Earth's. But available geological and geochemical evidence suggests that oxygenic photosynthesis appeared hundreds of millions of years before the oxygen-rich atmosphere was fully established. The interregnum implies that at least one other necessary condition for O2 had to be met. Here we argue that the second condition was the irreversible oxidation of the surface and crust by hydrogen escape. This is the same cause as it is for other planets with oxidized surfaces, such as Mars. The link between hydrogen escape and oxygen is direct because the primary process in oxygenic photosynthesis is splitting H2O into hydrogen and O2. Gradual oxidation of the surface and crust eventually brought Earth to a tipping point where free O2 became more stable than competing reduced gases such as CH4; or put another way, the system evolved to the point where surface oxidation under a reducing atmosphere could not keep pace with hydrogen escape. Because hydrogen escape is no faster than other geological processes that govern the long-term redox budget of the atmosphere, the approach to oxygen's final triumph would have been fitful and punctuated by episodes of oxygenation, as the record suggests was the case. The duration of the interregnum was determined by the rate of hydrogen escape and by the size of the reduced reservoir that needed to be oxidized before O2 became favored. If hydrogen escape was responsible for O2, it may be possible to account for the rough constancy of del 13C as a rough constancy of the H2/CO2 ratio in volcanic gases that follows from the constancy of the mantle's oxygen fugacity and a rough constancy in the H2O/CO2 ratio in subducted materials.

A DEEP HUBBLE SPACE TELESCOPE SEARCH FOR ESCAPING LYMAN CONTINUUM FLUX AT z {approx} 1.3: EVIDENCE FOR AN EVOLVING IONIZING EMISSIVITY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Siana, Brian; Bridge, Carrie R.; Teplitz, Harry I.

We have obtained deep Hubble Space Telescope far-UV images of 15 starburst galaxies at z {approx} 1.3 in the GOODS fields to search for escaping Lyman continuum (LyC) photons. These are the deepest far-UV images (m{sub AB} = 28.7, 3{sigma}, 1'' diameter) over this large an area (4.83 arcmin{sup 2}) and provide some of the best escape fraction constraints for any galaxies at any redshift. We do not detect any individual galaxies, with 3{sigma} limits to the LyC ({approx}700 A) flux 50-149 times fainter (in f{sub {nu}}) than the rest-frame UV (1500 A) continuum fluxes. Correcting for the mean intergalacticmore » medium (IGM) attenuation (factor {approx}2), as well as an intrinsic stellar Lyman break (factor {approx}3), these limits translate to relative escape fraction limits of f{sub esc,rel} < [0.03, 0.21]. The stacked limit is f{sub esc,rel}(3{sigma}) < 0.02. We use a Monte Carlo simulation to properly account for the expected distribution of line-of-sight IGM opacities. When including constraints from previous surveys at z {approx} 1.3 we find that, at the 95% confidence level, no more than 8% of star-forming galaxies at z {approx} 1.3 can have relative escape fractions greater than 0.50. Alternatively, if the majority of galaxies have low, but non-zero, escaping LyC, the escape fraction cannot be more than 0.04. In light of some evidence for strong LyC emission from UV-faint regions of Lyman break galaxies (LBGs) at z {approx} 3, we also stack sub-regions of our galaxies with different surface brightnesses and detect no significant LyC flux at the f{sub esc,rel} < 0.03 level. Both the stacked limits and the limits from the Monte Carlo simulation suggest that the average ionizing emissivity (relative to non-ionizing UV emissivity) at z {approx} 1.3 is significantly lower than has been observed in LBGs at z {approx} 3. If the ionizing emissivity of star-forming galaxies is in fact increasing with redshift, it would help to explain the high photoionization rates seen in the IGM at z>4 and reionization of the IGM at z>6.« less

Flight Performance Feasibility Studies for the Max Launch Abort System

NASA Technical Reports Server (NTRS)

Tarabini, Paul V.; Gilbert, Michael G.; Beaty, James R.

2013-01-01

In 2007, the NASA Engineering and Safety Center (NESC) initiated the Max Launch Abort System Project to explore crew escape system concepts designed to be fully encapsulated within an aerodynamic fairing and smoothly integrated onto a launch vehicle. One objective of this design was to develop a more compact launch escape vehicle that eliminated the need for an escape tower, as was used in the Mercury and Apollo escape systems and what is planned for the Orion Multi-Purpose Crew Vehicle (MPCV). The benefits for the launch vehicle of eliminating a tower from the escape vehicle design include lower structural weights, reduced bending moments during atmospheric flight, and a decrease in induced aero-acoustic loads. This paper discusses the development of encapsulated, towerless launch escape vehicle concepts, especially as it pertains to the flight performance and systems analysis trade studies conducted to establish mission feasibility and assess system-level performance. Two different towerless escape vehicle designs are discussed in depth: one with allpropulsive control using liquid attitude control thrusters, and a second employing deployable aft swept grid fins to provide passive stability during coast. Simulation results are presented for a range of nominal and off-nominal escape conditions.

Photochemical escape of oxygen from Mars: First results from MAVEN in situ data

NASA Astrophysics Data System (ADS)

Lillis, Robert J.; Deighan, Justin; Fox, Jane L.; Bougher, Stephen W.; Lee, Yuni; Combi, Michael R.; Cravens, Thomas E.; Rahmati, Ali; Mahaffy, Paul R.; Benna, Mehdi; Elrod, Meredith K.; McFadden, James P.; Ergun, Robert. E.; Andersson, Laila; Fowler, Christopher M.; Jakosky, Bruce M.; Thiemann, Ed; Eparvier, Frank; Halekas, Jasper S.; Leblanc, François; Chaufray, Jean-Yves

2017-03-01

Photochemical escape of atomic oxygen is thought to be one of the dominant channels for Martian atmospheric loss today and played a potentially major role in climate evolution. Mars Atmosphere and Volatile Evolution Mission (MAVEN) is the first mission capable of measuring, in situ, the relevant quantities necessary to calculate photochemical escape fluxes. We utilize 18 months of data from three MAVEN instruments: Langmuir Probe and Waves, Neutral Gas and Ion Mass Spectrometer, and SupraThermal And Thermal Ion Composition. From these data, we calculate altitude profiles of the production rate of hot oxygen atoms from the dissociative recombination of O2+ and the probability that such atoms will escape the Mars atmosphere. From this, we determine escape fluxes for 815 periapsis passes. Derived average dayside hot O escape rates range from 1.2 to 5.5 × 1025 s-1, depending on season and EUV flux, consistent with several pre-MAVEN predictions and in broad agreement with estimates made with other MAVEN measurements. Hot O escape fluxes do not vary significantly with dayside solar zenith angle or crustal magnetic field strength but depend on CO2 photoionization frequency with a power law whose exponent is 2.6 ± 0.6, an unexpectedly high value which may be partially due to seasonal and geographic sampling. From this dependence and historical EUV measurements over 70 years, we estimate a modern-era average escape rate of 4.3 × 1025 s-1. Extrapolating this dependence to early solar system, EUV conditions gives total losses of 13, 49, 189, and 483 mbar of oxygen over 1-3 and 3.5 Gyr, respectively, with uncertainties significantly increasing with time in the past.

Hydrogen escape from Mars enhanced by deep convection in dust storms

NASA Astrophysics Data System (ADS)

Heavens, Nicholas G.; Kleinböhl, Armin; Chaffin, Michael S.; Halekas, Jasper S.; Kass, David M.; Hayne, Paul O.; McCleese, Daniel J.; Piqueux, Sylvain; Shirley, James H.; Schofield, John T.

2018-02-01

Present-day water loss from Mars provides insight into Mars's past habitability1-3. Its main mechanism is thought to be Jeans escape of a steady hydrogen reservoir sourced from odd-oxygen reactions with near-surface water vapour2, 4,5. The observed escape rate, however, is strongly variable and correlates poorly with solar extreme-ultraviolet radiation flux6-8, which was predicted to modulate escape9. This variability has recently been attributed to hydrogen sourced from photolysed middle atmospheric water vapour10, whose vertical and seasonal distribution is only partly characterized and understood11-13. Here, we report multi-annual observational estimates of water content and dust and water transport to the middle atmosphere from Mars Climate Sounder data. We provide strong evidence that the transport of water vapour and ice to the middle atmosphere by deep convection in Martian dust storms can enhance hydrogen escape. Planet-encircling dust storms can raise the effective hygropause (where water content rapidly decreases to effectively zero) from 50 to 80 km above the areoid (the reference equipotential surface). Smaller dust storms contribute to an annual mode in water content at 40-50 km that may explain seasonal variability in escape. Our results imply that Martian atmospheric chemistry and evolution can be strongly affected by the meteorology of the lower and middle atmosphere of Mars.

5. NORTH REAR, EAST PART, SHOWING ESCAPE HATCH. TEST STAND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. NORTH REAR, EAST PART, SHOWING ESCAPE HATCH. TEST STAND 1-3 AND ITS MACHINE SHOP ARE IN MIDDLE DISTANCE. - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Instrumentation & Control Building, Test Area 1-115, northwest end of Saturn Boulevard, Boron, Kern County, CA

Systems Engineering Processes Applied to Ground Vehicle Integration at US Army Tank Automotive Research, Development, and Engineering Center (TARDEC)

DTIC Science & Technology

2010-08-19

UNCLASSIFIED Systems Engineering Processes Applied To Ground Vehicle Integration at US Army Tank Automotive Research, Development, and Engineering...DATES COVERED - 4. TITLE AND SUBTITLE Systems Engineering Processes Applied To Ground Vehicle Integration at US Army Tank Automotive Research...release, distribution unlimited 13. SUPPLEMENTARY NOTES Presented at NDIAs Ground Vehicle Systems Engineering and Technology Symposium (GVSETS), 17 22

Almost certain escape from black holes in final state projection models.

PubMed

Lloyd, Seth

2006-02-17

Recent models of the black-hole final state suggest that quantum information can escape from a black hole by a process akin to teleportation. These models rely on a controversial process called final-state projection. This Letter discusses the self-consistency of the final-state projection hypothesis and investigates escape from black holes for arbitrary final states and for generic interactions between matter and Hawking radiation. Quantum information escapes with fidelity approximately = (8/3pi)2: only half a bit of quantum information is lost on average, independent of the number of bits that escape from the hole.

M13 bacteriophage displaying DOPA on surfaces: fabrication of various nanostructured inorganic materials without time-consuming screening processes.

PubMed

Park, Joseph P; Do, Minjae; Jin, Hyo-Eon; Lee, Seung-Wuk; Lee, Haeshin

2014-01-01

M13 bacteriophage (phage) was engineered for the use as a versatile template for preparing various nanostructured materials via genetic engineering coupled to enzymatic chemical conversions. First, we engineered the M13 phage to display TyrGluGluGlu (YEEE) on the pVIII coat protein and then enzymatically converted the Tyr residue to 3,4-dihydroxyl-l-phenylalanine (DOPA). The DOPA-displayed M13 phage could perform two functions: assembly and nucleation. The engineered phage assembles various noble metals, metal oxides, and semiconducting nanoparticles into one-dimensional arrays. Furthermore, the DOPA-displayed phage triggered the nucleation and growth of gold, silver, platinum, bimetallic cobalt-platinum, and bimetallic iron-platinum nanowires. This versatile phage template enables rapid preparation of phage-based prototype devices by eliminating the screening process, thus reducing effort and time.

Quality control process improvement of flexible printed circuit board by FMEA

NASA Astrophysics Data System (ADS)

Krasaephol, Siwaporn; Chutima, Parames

2018-02-01

This research focuses on the quality control process improvement of Flexible Printed Circuit Board (FPCB), centred around model 7-Flex, by using Failure Mode and Effect Analysis (FMEA) method to decrease proportion of defective finished goods that are found at the final inspection process. Due to a number of defective units that were found at the final inspection process, high scraps may be escaped to customers. The problem comes from poor quality control process which is not efficient enough to filter defective products from in-process because there is no In-Process Quality Control (IPQC) or sampling inspection in the process. Therefore, the quality control process has to be improved by setting inspection gates and IPCQs at critical processes in order to filter the defective products. The critical processes are analysed by the FMEA method. IPQC is used for detecting defective products and reducing chances of defective finished goods escaped to the customers. Reducing proportion of defective finished goods also decreases scrap cost because finished goods incur higher scrap cost than work in-process. Moreover, defective products that are found during process can reflect the abnormal processes; therefore, engineers and operators should timely solve the problems. Improved quality control was implemented for 7-Flex production lines from July 2017 to September 2017. The result shows decreasing of the average proportion of defective finished goods and the average of Customer Manufacturers Lot Reject Rate (%LRR of CMs) equal to 4.5% and 4.1% respectively. Furthermore, cost saving of this quality control process equals to 100K Baht.

Surface-Wave Data Acquisition and Dissemination by VHF Packet Radio and Computer Networking

DTIC Science & Technology

1988-04-01

winter. , 4.3 Installation and Checkout The van was installed at the site by using a 3/4 ton pickup with a hydraulic tail gate; the van was simply lowered...13 ESCAPE OFF STREAMSW $7C FLOW ON STREAMCA OFF FRACK 4 STREAMCA OFF FULLDUP OFF STREAMDB OFF HEADERLN OFF TRFLOW OFFHID OFF TRIES 0 LCOK OFF TRACE...OFF RESPTIME 12 v DWAIT 1 SCREENLN 80 DIGIPEAT OFF SENDPAC SOD ECHO OFF START $11 ESCAPE OFF STOP $13 FLOW OFF STREAMSW $7C FRACK 4 STREAMCA OFF

Mercury Capsule Model in the 1- by 1-Foot Supersonic Wind Tunnel

NASA Image and Video Library

1959-10-21

National Aeronautics and Space Administration (NASA) researchers install a small-scale model of the capsule for Project Mercury in the 1- by 1-Foot Supersonic Wind Tunnel at the Lewis Research Center. NASA Lewis conducted a variety of tests for Project Mercury, including retrorocket calibration, escape tower engine performance, and separation of the capsule from simulated Atlas and Redstone boosters. The test of this capsule and escape tower model in the 1- by 1-foot tunnel were run in January and February 1960. The 1-by 1-Foot Supersonic Wind Tunnel had a 15-inch long test section, seen here, that was one foot wide and one foot high. The sides were made of glass to allow cameras to capture the supersonic air flow over the models. The tunnel could generate air flows from Mach 1.3 to 3.0. At the time, it was one of nine small supersonic wind tunnels at Lewis. These tunnels used the exhauster and compressor equipment of the larger facilities. The 1- by 1 tunnel, which began operating in the early 1950s, was built inside a test cell in the expansive Engine Research Building. During the 1950s the 1- by 1 was used to study a variety of inlets, nozzles, and cones for missiles and scramjets. The Mercury capsule tests were among the last at the facility for many years. The tunnel was mothballed in 1960. The 1- by 1 was briefly restored in 1972, then brought back online for good in 1979. The facility has maintained a brisk operating schedule ever since.

Development of a water-escape motivated version of the Stone T-maze for mice

PubMed Central

Pistell, Paul J.; Ingram, Donald K.

2014-01-01

Mice provide a highly valuable resource for investigating learning and memory processes; however, many of the established tasks for evaluating learning and memory were developed for rats. Behaviors of mice in these tasks appear to be driven by different motivational factors, and as a result, they often do not perform reliably on tasks involving rewards traditionally used for rats. Because of difficulties in measuring learning and memory in mice as well as the need to have a task that can reliably measure these behavioral processes, we have developed a mouse version of the Stone T-maze utilizing what appears to be the primary motivation of mice, escape to a safe location. Specifically, we have constructed a task that requires the mouse to wade through water to reach a dark and dry goal box. To escape this aversive environment, the Stone T-maze requires learning the correct sequence of 13 left and right turns to reach the goal box. Through a series of experiments examining a variety of protocols, it was found that mice will reliably perform this task. This task can be used to assess learning and memory without the potential performance confounds that can affect performance of mice in other tasks. We believe this task offers a valuable new tool for evaluating learning and memory in mice not previously available to researchers. PMID:20026250

Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement

PubMed Central

Sorce, Barbara; Escobedo, Carlos; Toyoda, Yusuke; Stewart, Martin P.; Cattin, Cedric J.; Newton, Richard; Banerjee, Indranil; Stettler, Alexander; Roska, Botond; Eaton, Suzanne; Hyman, Anthony A.; Hierlemann, Andreas; Müller, Daniel J.

2015-01-01

Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject cells to lateral mechanical confinement similar to that experienced in epithelia. If generating sufficient force to deform the pillars, rounding epithelial (MDCK) cells can create space to divide. However, if mitotic cells cannot create sufficient space, their rounding force, which is generated by actomyosin contraction and hydrostatic pressure, pushes the cell out of confinement. After conducting mitosis in an unperturbed manner, both daughter cells return to the confinement of the pillars. Cells that cannot round against nor escape confinement cannot orient their mitotic spindles and more likely undergo apoptosis. The results highlight how spatially constrained epithelial cells prepare for mitosis: either they are strong enough to round up or they must escape. The ability to escape from confinement and reintegrate after mitosis appears to be a basic property of epithelial cells. PMID:26602832

Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement

NASA Astrophysics Data System (ADS)

Sorce, Barbara; Escobedo, Carlos; Toyoda, Yusuke; Stewart, Martin P.; Cattin, Cedric J.; Newton, Richard; Banerjee, Indranil; Stettler, Alexander; Roska, Botond; Eaton, Suzanne; Hyman, Anthony A.; Hierlemann, Andreas; Müller, Daniel J.

2015-11-01

Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject cells to lateral mechanical confinement similar to that experienced in epithelia. If generating sufficient force to deform the pillars, rounding epithelial (MDCK) cells can create space to divide. However, if mitotic cells cannot create sufficient space, their rounding force, which is generated by actomyosin contraction and hydrostatic pressure, pushes the cell out of confinement. After conducting mitosis in an unperturbed manner, both daughter cells return to the confinement of the pillars. Cells that cannot round against nor escape confinement cannot orient their mitotic spindles and more likely undergo apoptosis. The results highlight how spatially constrained epithelial cells prepare for mitosis: either they are strong enough to round up or they must escape. The ability to escape from confinement and reintegrate after mitosis appears to be a basic property of epithelial cells.

Sesquinary Catenae on the Martian Satellite Phobos from Reaccretion of Escaping Ejecta

DTIC Science & Technology

2016-08-30

Life near the Roche limit—behavior of ejecta from satellites close to planets . Icarus 42, 422–441 (1980). 13. Soter, S. in Studies of the Terrestrial...sesquinaries are probes of the primary ejection process, but are also bound to the dynamics of the planet -satellite system. Unlike secondaries, to...intermediate between vesc and the orbital velocity vorb. When the satellite is far from the planet , sesquinaries can produce primary- like crater morphology

Why an intrinsic magnetic field does not protect a planet against atmospheric escape

NASA Astrophysics Data System (ADS)

Gunell, Herbert; Maggiolo, Romain; Nilsson, Hans; Stenberg Wieser, Gabriella; Slapak, Rikard; Lindkvist, Jesper; Hamrin, Maria; De Keyser, Johan

2018-06-01

The presence or absence of a magnetic field determines the nature of how a planet interacts with the solar wind and what paths are available for atmospheric escape. Magnetospheres form both around magnetised planets, such as Earth, and unmagnetised planets, like Mars and Venus, but it has been suggested that magnetised planets are better protected against atmospheric loss. However, the observed mass escape rates from these three planets are similar (in the approximate (0.5-2) kg s-1 range), putting this latter hypothesis into question. Modelling the effects of a planetary magnetic field on the major atmospheric escape processes, we show that the escape rate can be higher for magnetised planets over a wide range of magnetisations due to escape of ions through the polar caps and cusps. Therefore, contrary to what has previously been believed, magnetisation is not a sufficient condition for protecting a planet from atmospheric loss. Estimates of the atmospheric escape rates from exoplanets must therefore address all escape processes and their dependence on the planet's magnetisation.

Comment on "A hydrogen-rich early Earth atmosphere".

PubMed

Catling, David C

2006-01-06

Tian et al. (Reports, 13 May 2005, p. 1014) proposed a hydrogen-rich early atmosphere with slow hydrogen escape from a cold thermosphere. However, their model neglects the ultraviolet absorption of all gases other than H2. The model also neglects Earth's magnetic field, which affects the temperature and density of ions and promotes nonthermal escape of neutral hydrogen.

Development of three-layered rumen escapable capsules for cattle

PubMed Central

SEYAMA, Tomohiro; HIRAYASU, Hirofumi; YAMAWAKI, Kenji; ADACHI, Takuhiko; SUGIMOTO, Takayuki; KASAI, Koji

2016-01-01

A new rumen escapable tool is presented for cattle in prospect of developing medical treatment or supplementing trace elements for disease prevention. This tool consists of a three-layered capsule that dissolves in the lower digestive tract, but not in the rumen. The capsule was manufactured by capsule-forming techniques through the use of liquid surface tension. This method does not involve high-temperature treatment, so the capsule can contain not only lipophilic substances but also hydrophilic or heat-sensitive substances. Furthermore, the capsule has a specific gravity of 1.3 and diameter of 6.0 mm, which were previously shown to be appropriate to avoid rumination. The objective of this study was to confirm the effectiveness of the capsule pertinent to rumen escaping. In order to validate rumen escape, capsules containing 30 g of water-soluble vitamin (thiamine hydrochloride) per head were administered to four lactating cows assigned in a crossover trial. In the group administered encapsulated thiamine hydrochloride, blood thiamine levels increased from 12.4 ± 1.03 ng/ml before administration to 54.8 ± 2.21 ng/ml at 6 hr following administration, whereas the level remained at 13.3 ± 2.05 ng/ml in the control group administered via aqueous solution. This indicates that the three-layered capsules passed through the rumen and dissolved in the lower digestive tract, thus functioning as a rumen escapable tool. PMID:27546371

Rational Engineering and Characterization of an mAb that Neutralizes Zika Virus by Targeting a Mutationally Constrained Quaternary Epitope.

PubMed

Tharakaraman, Kannan; Watanabe, Satoru; Chan, Kuan Rong; Huan, Jia; Subramanian, Vidya; Chionh, Yok Hian; Raguram, Aditya; Quinlan, Devin; McBee, Megan; Ong, Eugenia Z; Gan, Esther S; Tan, Hwee Cheng; Tyagi, Anu; Bhushan, Shashi; Lescar, Julien; Vasudevan, Subhash G; Ooi, Eng Eong; Sasisekharan, Ram

2018-05-09

Following the recent emergence of Zika virus (ZIKV), many murine and human neutralizing anti-ZIKV antibodies have been reported. Given the risk of virus escape mutants, engineering antibodies that target mutationally constrained epitopes with therapeutically relevant potencies can be valuable for combating future outbreaks. Here, we applied computational methods to engineer an antibody, ZAb_FLEP, that targets a highly networked and therefore mutationally constrained surface formed by the envelope protein dimer. ZAb_FLEP neutralized a breadth of ZIKV strains and protected mice in distinct in vivo models, including resolving vertical transmission and fetal mortality in infected pregnant mice. Serial passaging of ZIKV in the presence of ZAb_FLEP failed to generate viral escape mutants, suggesting that its epitope is indeed mutationally constrained. A single-particle cryo-EM reconstruction of the Fab-ZIKV complex validated the structural model and revealed insights into ZAb_FLEP's neutralization mechanism. ZAb_FLEP has potential as a therapeutic in future outbreaks. Copyright © 2018. Published by Elsevier Inc.

The atmospheric escape at Mars: complementing the scenario

NASA Astrophysics Data System (ADS)

Lilensten, Jean; Simon, Cyril; Barthélémy, Mathieu; Thissen, Roland; Ehrenreich, David; Gronoff, Guillaume; Witasse, Olivier

2013-04-01

In the recent years, the presence of dications in the atmospheres of Mars, Venus, Earth and Titan has been modeled and assessed. These studies also suggested that these ions could participate to the escape of the planetary atmospheres because a large fraction of them is unstable and highly ener- getic. When they dissociate, their internal energy is transformed into kinetic energy which may be larger than the escape energy. This study assesses the impact of the doubly-charged ions in the escape of CO2-dominated planetary atmospheres and to compare it to the escape of thermal photo-ions.We solve a Boltzmann transport equation at daytime taking into account the dissociative states of CO++ for a simplified single constituent atmosphere of a 2 case-study planet. We compute the escape of fast ions using a Beer-Lambert approach. We study three test-cases. On a Mars-analog planet in today's conditions, we retrieve the measured electron escape flux. When comparing the two mechanisms (i.e. excluding solar wind effects, sputtering ...), the escape due to the fast ions issuing from the dissociation of dications may account for up to 6% of the total and the escape of thermal ions for the remaining. We show that these two mechanisms cannot explain the escape of the atmosphere since the magnetic field vanished but complement the other processes and allow writing the scenario of the Mars escape. We show that the atmosphere of a Mars analog planet would empty in another giga years and a half. At Venus orbit, the contribution of the dications in the escape rate is negligible.When simulating the hot Jupiter HD209458b, the two processes cannot explain the measured escape flux of C+.

Improved Design Process Part of the Ship Producibility Program of the National Shipbuilding Research Program

DTIC Science & Technology

1977-04-01

Exh and Escape Fresh Water Feed Treatment Cargo Oil Tank Cleaning Diesel Generator Piping Piping Material Schedule List of Motors and... tank length frame and stiffener spacing, etc. One yard buys only mill edge plates, and one yard buys only cold flange quality plates. 2-13 C. POST...gage boards, all interconnecting piping and valves, and all mounted on a common foundation, or a pump room assembled on a tank top unit. Use of packages

On the escape of oxygen and hydrogen from Mars

NASA Technical Reports Server (NTRS)

Fox, J. L.

1993-01-01

Escape rates of oxygen atoms from dissociative recombination of O2(+) above the Martian exobase are computed in light of new information from ab initio calculations of the dissociative recombination process and our recently revised understanding of the Martian dayside ionosphere. Only about 60 percent of the dissociative recombinations occur in channels in which the O atoms are released with energies in excess of the escape velocity. Futhermore, we find that the computed escape fluxes for O depend greatly on the nature of the ion loss process that has been found necessary to reproduce the topside ion density profiles measured by Viking. If it is assumed that the ions are not lost from the gravitational field of the planet, as required by an analysis of nitrogen escape, the computed average O escape rate is 3 x 10 exp 6/sq cm/s, much less than half the H escape rates inferred from measurements of the Lyman-alpha dayglow, which are in the range (1-2) x 10 exp 8/sq cm/s. Suggestions for restoring the relative escape rates of H and O to the stoichiometric ratio of water are explored.

High Resolution Observations of Escaping Ions in the Martian Magnetotail

NASA Astrophysics Data System (ADS)

Halekas, J. S.; Raman, C.; Brain, D.; DiBraccio, G. A.; Harada, Y.; McFadden, J. P.; Mitchell, D. L.; Connerney, J. E. P.; Jakosky, B. M.

2016-12-01

Ions escape from the Martian upper atmosphere via a number of channels, including the central plasmasheet of the magnetotail. Mars Express observations show that the heavy ions O+ and O2+ escaping through the central tail often have approximately the same energy, suggesting acceleration in a quasi-static electric field, which has been interpreted as a Hall electric field. The Solar Wind Ion Analyzer (SWIA) on MAVEN was designed to measure the upstream solar wind. However, during orbit segments with appropriate spacecraft attitude, SWIA can also make high resolution measurements of escaping ions in the tail. During the prime mission, these observations were only returned sporadically, during periods of intense escaping fluxes that fortuitously triggered a mode switch. Now, in the extended mission, we return high resolution observations from SWIA routinely. Some of these high resolution measurements reveal slight differences in both the direction and energy of escaping O+ and O2+ ions, which may help determine the acceleration process(es). We investigate the location and solar wind conditions for which the escaping ions separate in energy and angle and the systematics of their energies and flow vectors, and discuss the implications for ion acceleration and the overall picture of Martian atmospheric escape.

Including natural systems into the system engineering process: benefits to spaceflight and beyond

NASA Astrophysics Data System (ADS)

Studor, George

2014-03-01

How did we get to the point where we don't have time to be inspired by the wonders of Nature? Our office walls, homes and city streets are so plain that even when we do escape to a retreat with nature all around us, we may be blind to its magnificence. Yet there are many who have applied what can be known of natural systems (NS) to create practical solutions, but often definite applications for them are lacking. Mimicry of natural systems is not only more possible than ever before, but the education and research programs in many major universities are churning out graduates with a real appreciation for Nature's complex integrated systems. What if these skills and perspectives were employed in the teams of systems engineers and the technology developers that support them to help the teams think "outside-the-box" of manmade inventions? If systems engineers (SE) and technology developers regularly asked the question, "what can we learn from Nature that will help us?" as a part of their processes, they would discover another set of potential solutions. Biomimicry and knowledge of natural systems is exploding. What does this mean for systems engineering and technology? Some disciplines such as robotics and medical devices must consider nature constantly. Perhaps it's time for all technology developers and systems engineers to perceive natural systems experts as potential providers of the technologies they need.

Increased Antibody Affinity Confers Broad In Vitro Protection against Escape Mutants of Severe Acute Respiratory Syndrome Coronavirus

PubMed Central

Rani, Mridula; Bolles, Meagan; Donaldson, Eric F.; Van Blarcom, Thomas; Baric, Ralph; Iverson, Brent

2012-01-01

Even though the effect of antibody affinity on neutralization potency is well documented, surprisingly, its impact on neutralization breadth and escape has not been systematically determined. Here, random mutagenesis and DNA shuffling of the single-chain variable fragment of the neutralizing antibody 80R followed by bacterial display screening using anchored periplasmic expression (APEx) were used to generate a number of higher-affinity variants of the severe acute respiratory syndrome coronavirus (SARS-CoV)-neutralizing antibody 80R with equilibrium dissociation constants (KD) as low as 37 pM, a >270-fold improvement relative to that of the parental 80R single-chain variable fragment (scFv). As expected, antigen affinity was shown to correlate directly with neutralization potency toward the icUrbani strain of SARS-CoV. Additionally, the highest-affinity antibody fragment displayed 10-fold-increased broad neutralization in vitro and completely protected against several SARS-CoV strains containing substitutions associated with antibody escape. Importantly, higher affinity also led to the suppression of viral escape mutants in vitro. Escape from the highest-affinity variant required reduced selective pressure and multiple substitutions in the binding epitope. Collectively, these results support the hypothesis that engineered antibodies with picomolar dissociation constants for a neutralizing epitope can confer escape-resistant protection. PMID:22696652

Wind Enhanced Escape, Ion Pickup and the Evolution of Water on Mars

NASA Technical Reports Server (NTRS)

Hartle, Richard

1999-01-01

Preferential loss of hydrogen over deuterium from Mars has produced a deuterium rich atmosphere possessing a D/B ratio 5.2 times that of terrestrial water. Rayleigh fractionation is applied, constrained by the deuterium enrichment factor, to determine the magnitudes of ancient and present water reservoirs on the planet. The dominant lose mechanisms of R and D from the current atmosphere are thought to be thermal escape and solar wind ion pickup of the neutral and ion forms of theme constituents, respectively. During an earlier martian epoch, only thermal escape was significant because Mars had a terrestrial sized magnetosphere that protected the atmosphere from solar wind scavenging processes. The magnitudes of present and ancient water reservoirs are estimated when thermal escape is considered alone and subsequently when the effects of ion pickup are added. The escape fluxes of R and D are significantly increased above the respective Jeans fluxes when the effects of thermospheric winds and planetary rotation are accounted for at the exobase. Such wind enhanced escape also increases as the mass of an escaping constituent increases; thus, the increase in the escape flux of D is greater than that of H. When the fractionation process is also constrained by the D/H ratio observed in hydrous minerals of SNC meteorites, an ancient crustal reservoir of Martian water in derived, tens of meters in global-equivalent depth, considerably exceeding that obtained with no winds. The reservoir becomes even larger when ion pickup processes are added.

Verge and Foliot Clock Escapement: A Simple Dynamical System

NASA Astrophysics Data System (ADS)

Denny, Mark

2010-09-01

The earliest mechanical clocks appeared in Europe in the 13th century. From about 1250 CE to 1670 CE, these simple clocks consisted of a weight suspended from a rope or chain that was wrapped around a horizontal axle. To tell time, the weight must fall with a slow uniform speed, but, under the action of gravity alone, such a suspended weight would accelerate. To prevent this acceleration, an escapement mechanism was required. The best such escapement mechanism was called the verge and foliot escapement, and it was so successful that it lasted until about 1800 CE. These simple weight-driven clocks with verge and foliot escapements were accurate enough to mark the hours but not minutes or seconds. From 1670, significant improvements were made (principally by introducing pendulums and the newly invented anchor escapement) that justified the introduction of hands to mark minutes, and then seconds. By the end of the era of mechanical clocks, in the first half of the 20th century, these much-studied and much-refined machines were accurate to a millisecond a day.

New insights on the collisional escape of light neutrals from Mars

NASA Astrophysics Data System (ADS)

Gacesa, Marko; Zahnle, Kevin

2017-04-01

Photodissociative recombination (PDR) of atmospheric molecules on Mars is a major mechanism of production of hot (suprathermal) atoms with sufficient kinetic energy to either directly escape to space or to eject other atmospheric species. This collisional ejection mechanism is important for evaluating the escape rates of all light neutrals that are too heavy to escape via Jeans escape. In particular, it plays a role in estimating the total volume of escaped water constituents (i.e., O and H) from Mars, as well as influences evolution of the atmospheric [D]/[H] ratio1. We present revised estimates of total collisional escape rates of neutral light elements including H, He, and H2, based on recent (years 2015-2016) atmospheric density profiles obtained from the NASA Mars Atmosphere and Volatile Evolution (MAVEN) mission. We also estimate the contribution to the collisional escape from Energetic Neutral Atoms (ENAs) produced in charge-exchange of solar wind H+ and He+ ions with atmospheric gases2,3. Scattering of hot oxygen and atmospheric species of interest is modeled using fully-quantum reactive scattering formalism1,3. The escape rates are evaluated using a 1D model of the atmosphere supplemented with MAVEN measurements of the neutrals. Finally, new estimates of contributions of these non-thermal mechanisms to the estimated PDR escape rates from young Mars4 are presented. [1] M. Gacesa and V. Kharchenko, "Non-thermal escape of molecular hydrogen from Mars", Geophys. Res. Lett., 39, L10203 (2012). [2] N. Lewkow and V. Kharchenko, "Precipitation of Energetic Neutral Atoms and Escape Fluxes induced from the Mars Atmosphere", Astroph. J., 790, 98 (2014). [3] M. Gacesa, N. Lewkow, and V. Kharchenko, "Non-thermal production and escape of OH from the upper atmosphere of Mars", Icarus 284, 90 (2017). [4] J. Zhao, F. Tian, Y. Ni, and X. Huang, "DR-induced escape of O and C from early Mars", Icarus 284, 305 (2017).

The uncertain response in humans and animals

NASA Technical Reports Server (NTRS)

Smith, J. D.; Shields, W. E.; Schull, J.; Washburn, D. A.; Rumbaugh, D. M. (Principal Investigator)

1997-01-01

There has been no comparative psychological study of uncertainty processes. Accordingly, the present experiments asked whether animals, like humans, escape adaptively when they are uncertain. Human and animal observers were given two primary responses in a visual discrimination task, and the opportunity to escape from some trials into easier ones. In one psychophysical task (using a threshold paradigm), humans escaped selectively the difficult trials that left them uncertain of the stimulus. Two rhesus monkeys (Macaca mulatta) also showed this pattern. In a second psychophysical task (using the method of constant stimuli), some humans showed this pattern but one escaped infrequently and nonoptimally. Monkeys showed equivalent individual differences. The data suggest that escapes by humans and monkeys are interesting cognitive analogs and may reflect controlled decisional processes prompted by the perceptual ambiguity at threshold.

Spacecraft Charging Issues for Launch Vehicles

NASA Technical Reports Server (NTRS)

Buhler, Janessa L.; Minow, Joseph I.; Trout, Dawn H.

2014-01-01

Spacecraft charging is well known threat to successful long term spacecraft operations and instrument reliability in orbits that spend significant time in hot electron environments. In recent years, spacecraft charging has increasingly been recognized as a potentially significant engineering issue for launch vehicles used to deploy spacecraft using (a) low Earth orbit (LEO), high inclination flight trajectories that pass through the auroral zone, (b) geostationary transfer orbits that require exposures to the hot electron environments in the Earths outer radiation belts, and (c) LEO escape trajectories using multiple phasing orbits through the Earths radiation belts while raising apogee towards a final Earth escape geometry. Charging becomes an issue when significant areas of exposed insulating materials or ungrounded conductors are used in the launch vehicle design or the payload is designed for use in a benign charging region beyond the Earths magnetosphere but must survive passage through the strong charging regimes of the Earths radiation belts. This presentation will first outline the charging risks encountered on typical launch trajectories used to deploy spacecraft into Earth orbit and Earth escape trajectories. We then describe the process used by NASAs Launch Services Program to evaluate when surface and internal charging is a potential risk to a NASA mission. Finally, we describe the options for mitigating charging risks including modification of the launch vehicle andor payload design and controlling the risk through operational launch constraints to avoid significant charging environments.

Hydrodynamic escape from planetary atmospheres

NASA Astrophysics Data System (ADS)

Tian, Feng

Hydrodynamic escape is an important process in the formation and evolution of planetary atmospheres. Due to the existence of a singularity point near the transonic point, it is difficult to find transonic steady state solutions by solving the time-independent hydrodynamic equations. In addition to that, most previous works assume that all energy driving the escape flow is deposited in one narrow layer. This assumption not only results in less accurate solutions to the hydrodynamic escape problem, but also makes it difficult to include other chemical and physical processes in the hydrodynamic escape models. In this work, a numerical model describing the transonic hydrodynamic escape from planetary atmospheres is developed. A robust solution technique is used to solve the time dependent hydrodynamic equations. The method has been validated in an isothermal atmosphere where an analytical solution is available. The hydrodynamic model is applied to 3 cases: hydrogen escape from small orbit extrasolar planets, hydrogen escape from a hydrogen rich early Earth's atmosphere, and nitrogen/methane escape from Pluto's atmosphere. Results of simulations on extrasolar planets are in good agreement with the observations of the transiting extrasolar planet HD209458b. Hydrodynamic escape of hydrogen from other hypothetical close-in extrasolar planets are simulated and the influence of hydrogen escape on the long-term evolution of these extrasolar planets are discussed. Simulations on early Earth suggest that hydrodynamic escape of hydrogen from a hydrogen rich early Earth's atmosphere is about two orders magnitude slower than the diffusion limited escape rate. A hydrogen rich early Earth's atmosphere could have been maintained by the balance between the hydrogen escape and the supply of hydrogen into the atmosphere by volcanic outgassing. Origin of life may have occurred in the organic soup ocean created by the efficient formation of prebiotic molecules in the hydrogen rich early Earth's atmosphere. Simulations show that hydrodynamic escape of nitrogen from Pluto is able to remove a ~3 km layer of ice over the age of the solar system. The escape flux of neutral nitrogen may interact with the solar wind at Pluto's orbit and may be detected by the New Horizon mission.

Single-File Escape of Colloidal Particles from Microfluidic Channels

NASA Astrophysics Data System (ADS)

Locatelli, Emanuele; Pierno, Matteo; Baldovin, Fulvio; Orlandini, Enzo; Tan, Yizhou; Pagliara, Stefano

2016-07-01

Single-file diffusion is a ubiquitous physical process exploited by living and synthetic systems to exchange molecules with their environment. It is paramount to quantify the escape time needed for single files of particles to exit from constraining synthetic channels and biological pores. This quantity depends on complex cooperative effects, whose predominance can only be established through a strict comparison between theory and experiments. By using colloidal particles, optical manipulation, microfluidics, digital microscopy, and theoretical analysis we uncover the self-similar character of the escape process and provide closed-formula evaluations of the escape time. We find that the escape time scales inversely with the diffusion coefficient of the last particle to leave the channel. Importantly, we find that at the investigated microscale, bias forces as tiny as 10-15 N determine the magnitude of the escape time by drastically reducing interparticle collisions. Our findings provide crucial guidelines to optimize the design of micro- and nanodevices for a variety of applications including drug delivery, particle filtering, and transport in geometrical constrictions.

Plasma Clouds and Snowplows: Bulk Plasma Escape from Mars Observed by MAVEN

NASA Technical Reports Server (NTRS)

Halekas, J. S.; Brain, D. A.; Ruhunusiri, S.; McFadden, J. P.; Mitchell, D. L.; Mazelle, C.; Connerney, J. E. P.; Harada, Y.; Hara, T.; Espley, J. R.;

Impact of Antiretroviral Regimens on CSF Viral Escape in a Prospective Multicohort Study of ART-Experienced HIV-1 Infected Adults in the United States.

PubMed

Mukerji, Shibani S; Misra, Vikas; Lorenz, David R; Uno, Hajime; Morgello, Susan; Franklin, Donald; Ellis, Ronald J; Letendre, Scott; Gabuzda, Dana

2018-04-03

Cerebrospinal fluid (CSF) viral escape occurs in 4-20% of HIV-infected adults, yet the impact of antiretroviral therapy (ART) on CSF escape is unclear. Prospective study of 1063 participants with baseline plasma viral load (VL) ≤400 copies/ml between 2005-2016. Odds ratio for ART regimens (PI with nucleoside reverse transcriptase inhibitor [PI+NRTI] versus other ART) and CSF escape was estimated using mixed-effects models. Drug resistance mutation frequencies were calculated. Baseline mean age was 46, median plasma VL, CD4 nadir, and CD4 count were 50 copies/mL, 88 cells/μL, and 424 cells/μL, respectively; 48% on PI+NRTI, 33% on non-NRTI, and 6% on integrase inhibitors. During median follow-up of 4.4 years, CSF escape occurred in 77 participants (7.2%). PI+NRTI use was an independent predictor of CSF escape (OR 3.1 [95% CI 1.8-5.0]) in adjusted analyses and models restricted to plasma VL ≤50 copies/ml (p<0.001). Regimens containing atazanavir (ATV) were a stronger predictor of CSF viral escape than non-ATV PI+NRTI regimens. Plasma and CSF M184V/I combined with thymidine-analog mutations were more frequent in CSF escape versus no escape (23% vs. 2.3%). Genotypic susceptibility score-adjusted CNS penetration-effectiveness (CPE) values were calculated for CSF escape with M184V/I mutations (n=34). Adjusted CPE values were low (<5) for CSF and plasma in 27 (79%) and 13 (38%), respectively, indicating suboptimal CNS drug availability. PI+NRTI regimens are independent predictors of CSF escape in HIV-infected adults. Reduced CNS ART bioavailability may predispose to CSF escape in patients with M184V/I mutations. Optimizing ART regimens may reduce risk of CSF escape.

Saturn Apollo Program

NASA Image and Video Library

1969-07-01

A technician can be seen working atop the white room across from the escape tower of the Apollo 11 spacecraft a few days prior to the launch of the Saturn V moon rocket. The towering 363-foot Saturn V was a multi-stage, multi-engine launch vehicle standing taller than the Statue of Liberty. Altogether, the Saturn V engines produced as much power as 85 Hoover Dams

MAVEN Observations of Escaping Planetary Ions from the Martian Atmosphere: Mass, Velocity, and Spatial Distributions

NASA Astrophysics Data System (ADS)

Dong, Yaxue; Fang, Xiaohua; Brain, D. A.; McFadden, James P.; Halekas, Jasper; Connerney, Jack

2015-04-01

The Mars-solar wind interaction accelerates and transports planetary ions away from the Martian atmosphere through a number of processes, including ‘pick-up’ by electromagnetic fields. The MAVEN spacecraft has made routine observations of escaping planetary ions since its arrival at Mars in September 2014. The SupraThermal And Thermal Ion Composition (STATIC) instrument measures the ion energy, mass, and angular spectra. It has detected energetic planetary ions during most of the spacecraft orbits, which are attributed to the pick-up process. We found significant variations in the escaping ion mass and velocity distributions from the STATIC data, which can be explained by factors such as varying solar wind conditions, contributions of particles from different source locations and different phases during the pick-up process. We also study the spatial distributions of different planetary ion species, which can provide insight into the physics of ion escaping process and enhance our understanding of atmospheric erosion by the solar wind. Our results will be further interpreted within the context of the upstream solar wind conditions measured by the MAVEN Solar Wind Ion Analyzer (SWIA) instrument and the magnetic field environment measured by the Magnetometer (MAG) instrument. Our study shows that the ion spatial distribution in the Mars-Sun-Electric-Field (MSE) coordinate system and the velocity space distribution with respect to the local magnetic field line can be used to distinguish the ions escaping through the polar plume and those through the tail region. The contribution of the polar plume ion escape to the total escape rate will also be discussed.

Transgenerational response to stress in plants and its application for breeding.

PubMed

Bilichak, Andriy; Kovalchuk, Igor

2016-03-01

A growing number of reports indicate that plants possess the ability to maintain a memory of stress exposure throughout their ontogenesis and even transmit it faithfully to the following generation. Some of the features of transgenerational memory include elevated genome instability, a higher tolerance to stress experienced by parents, and a cross-tolerance. Although the underlying molecular mechanisms of this phenomenon are not clear, a likely contributing factor is the absence of full-scale reprogramming of the epigenetic landscape during gametogenesis; therefore, epigenetic marks can occasionally escape the reprogramming process and can be passed on to the progeny. To date, it is not entirely clear which part of the epigenetic landscape is more likely to escape the reprogramming events, and whether such a process is random or directed and sequence specific. The identification of specific epigenetic marks associated with specific stressors would allow generation of stress-tolerant plants through the recently discovered techniques for precision epigenome engineering. The engineered DNA-binding domains (e.g. ZF, TALE, and dCas9) fused to particular chromatin modifiers would make it possible to target epigenetic modifications to the selected loci, probably allowing stress tolerance to be achieved in the progeny. This approach, termed epigenetic breeding, along with other methods has great potential to be used for both the assessment of the propagation of epigenetic marks across generations and trait improvement in plants. In this communication, we provide a short overview of recent reports demonstrating a transgenerational response to stress in plants, and discuss the underlying potential molecular mechanisms of this phenomenon and its use for plant biotechnology applications. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

13. VIEW, LOOKING WEST FROM LEFT TO RIGHT, SHOWING HIGH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. VIEW, LOOKING WEST FROM LEFT TO RIGHT, SHOWING HIGH PRESSURE AIR FLASK ROOM AND PUMP ROOM - U.S. Naval Submarine Base, New London Submarine Escape Training Tank, Albacore & Darter Roads, Groton, New London County, CT

Fitness-Balanced Escape Determines Resolution of Dynamic Founder Virus Escape Processes in HIV-1 Infection

PubMed Central

Sunshine, Justine E.; Larsen, Brendan B.; Maust, Brandon; Casey, Ellie; Deng, Wenje; Chen, Lennie; Westfall, Dylan H.; Kim, Moon; Zhao, Hong; Ghorai, Suvankar; Lanxon-Cookson, Erinn; Rolland, Morgane; Collier, Ann C.; Maenza, Janine; Mullins, James I.

2015-01-01

ABSTRACT To understand the interplay between host cytotoxic T-lymphocyte (CTL) responses and the mechanisms by which HIV-1 evades them, we studied viral evolutionary patterns associated with host CTL responses in six linked transmission pairs. HIV-1 sequences corresponding to full-length p17 and p24 gag were generated by 454 pyrosequencing for all pairs near the time of transmission, and seroconverting partners were followed for a median of 847 days postinfection. T-cell responses were screened by gamma interferon/interleukin-2 (IFN-γ/IL-2) FluoroSpot using autologous peptide sets reflecting any Gag variant present in at least 5% of sequence reads in the individual's viral population. While we found little evidence for the occurrence of CTL reversions, CTL escape processes were found to be highly dynamic, with multiple epitope variants emerging simultaneously. We found a correlation between epitope entropy and the number of epitope variants per response (r = 0.43; P = 0.05). In cases in which multiple escape mutations developed within a targeted epitope, a variant with no fitness cost became fixed in the viral population. When multiple mutations within an epitope achieved fitness-balanced escape, these escape mutants were each maintained in the viral population. Additional mutations found to confer escape but undetected in viral populations incurred high fitness costs, suggesting that functional constraints limit the available sites tolerable to escape mutations. These results further our understanding of the impact of CTL escape and reversion from the founder virus in HIV infection and contribute to the identification of immunogenic Gag regions most vulnerable to a targeted T-cell attack. IMPORTANCE Rapid diversification of the viral population is a hallmark of HIV-1 infection, and understanding the selective forces driving the emergence of viral variants can provide critical insight into the interplay between host immune responses and viral evolution. We used deep sequencing to comprehensively follow viral evolution over time in six linked HIV transmission pairs. We then mapped T-cell responses to explore if mutations arose due to adaption to the host and found that escape processes were often highly dynamic, with multiple mutations arising within targeted epitopes. When we explored the impact of these mutations on replicative capacity, we found that dynamic escape processes only resolve with the selection of mutations that conferred escape with no fitness cost to the virus. These results provide further understanding of the complicated viral-host interactions that occur during early HIV-1 infection and may help inform the design of future vaccine immunogens. PMID:26223634

A Proposed Ascent Abort Flight Test for the Max Launch Abort System

NASA Technical Reports Server (NTRS)

Tartabini, Paul V.; Gilbert, Michael G.; Starr, Brett R.

2016-01-01

The NASA Engineering and Safety Center initiated the Max Launch Abort System (MLAS) Project to investigate alternate crew escape system concepts that eliminate the conventional launch escape tower by integrating the escape system into an aerodynamic fairing that fully encapsulates the crew capsule and smoothly integrates with the launch vehicle. This paper proposes an ascent abort flight test for an all-propulsive towerless escape system concept that is actively controlled and sized to accommodate the Orion Crew Module. The goal of the flight test is to demonstrate a high dynamic pressure escape and to characterize jet interaction effects during operation of the attitude control thrusters at transonic and supersonic conditions. The flight-test vehicle is delivered to the required test conditions by a booster configuration selected to meet cost, manufacturability, and operability objectives. Data return is augmented through judicious design of the boost trajectory, which is optimized to obtain data at a range of relevant points, rather than just a single flight condition. Secondary flight objectives are included after the escape to obtain aerodynamic damping data for the crew module and to perform a high-altitude contingency deployment of the drogue parachutes. Both 3- and 6-degree-of-freedom trajectory simulation results are presented that establish concept feasibility, and a Monte Carlo uncertainty assessment is performed to provide confidence that test objectives can be met.

Measuring behaviours for escaping from house fires: use of latent variable models to summarise multiple behaviours.

PubMed

Ploubidis, G B; Edwards, P; Kendrick, D

2015-12-15

This paper reports the development and testing of a construct measuring parental fire safety behaviours for planning escape from a house fire. Latent variable modelling of data on parental-reported fire safety behaviours and plans for escaping from a house fire and multivariable logistic regression to quantify the association between groups defined by the latent variable modelling and parental-report of having a plan for escaping from a house fire. Data comes from 1112 participants in a cluster randomised controlled trial set in children's centres in 4 study centres in the UK. A two class model provided the best fit to the data, combining responses to five fire safety planning behaviours. The first group ('more behaviours for escaping from a house fire') comprised 86% of participants who were most likely to have a torch, be aware of how their smoke alarm sounds, to have external door and window keys accessible, and exits clear. The second group ('fewer behaviours for escaping from a house fire') comprised 14% of participants who were less likely to report these five behaviours. After adjusting for potential confounders, participants allocated to the 'more behaviours for escaping from a house fire group were 2.5 times more likely to report having an escape plan (OR 2.48; 95% CI 1.59-3.86) than those in the "fewer behaviours for escaping from a house fire" group. Multiple fire safety behaviour questions can be combined into a single binary summary measure of fire safety behaviours for escaping from a house fire. Our findings will be useful to future studies wishing to use a single measure of fire safety planning behaviour as measures of outcome or exposure. NCT 01452191. Date of registration 13/10/2011.

Modulation of Endosomal Escape of IRQ-PEGylated Nano-carrier

NASA Astrophysics Data System (ADS)

Mudhakir, Diky; Akita, Hidetaka; Harashima, Hideyoshi

2011-12-01

The novel IRQ peptide is one of cell penetrating peptides (CPPs) that has ability to induce endosomal escape. It has been demonstrated that IRQ ligand had ability to facilitate an escape of liposomes encapsulating siRNA from the endosomes presumably by fusion-independent mechanism [1,2]. In the present study, we attempted to modulate the intracellular trafficking of IRQ-modified nano-carrier in term of escaping process by changing the lipid composition. The peptide was attached to the terminal end of maleimide group of polyethylene glycol-modified liposomes (IRQ-PEG-Lip). The liposomes were composed of DOTAP, DOPE and cholesterol and it was labeled by water soluble sulpho-rhodamine B (Sr-B). The escape of PEG-coated liposomes was then observed by confocal laser scanning microscope after the endosomes were stained with Lysosensor. The results exhibited that IRQ-PEG-Lip was escaped from endosomal compartment after 1 h transfection when 40% of DOPE was incorporated into the nanostructure comparing to that of PEG-Lip. These results are consistent with the previous results that the IRQ facilitates endosomal escape via independent-mechanism. However, IRQ-PEG-Lip were then completely co-localized in the acidic compartment when density of DOPE was reduced approximately 20%. These results indicated that the utilizing of DOPE is important for the escape process even in the presence of hydrophilic PEG polymer. In conclusion, the regulation of endosomal escape ability of the PEGylated-IRQ nano-carrier was induced by fusion-independent manner as well as fusogenic lipid.

European policymaking on the tobacco advertising ban: the importance of escape routes.

PubMed

Adamini, Sandra; Versluis, Esther; Maarse, Hans

2011-01-01

This article analyses the European Union policymaking process regarding tobacco advertising. While others already highlighted the importance of intergovernmental bargaining between member states to explain the outcome of the tobacco advertising case, the main aim of this article is to identify the use of escape routes by the Commission, the European Parliament, the Council and interest groups that played an important role in overcoming the deadlock. When looking at the different institutions that structure policymaking, we argue that indeed focusing on escape routes provides a clear insight in the process and in what strategies were necessary to 'make Europe work'. In the end, it appears to be a combination of escape routes that resulted in the final decision.

Engineering Replacement Tissues with Amniotic Stem Cells

DTIC Science & Technology

2012-10-01

compression. J Biomech, 2010. 43(13): p. 2516-23. 17. Gadjanski, I., K. Spiller, and G. Vunjak- Novakovic , Time-dependent processes in stem cell-based...16. Gadjanski, I., K. Spiller, and G. Vunjak- Novakovic , Time-dependent processes in stem cell-based tissue engineering of articular cartilage. Stem

Means of escape provisions and evacuation simulation of public building in Malaysia and Singapore

NASA Astrophysics Data System (ADS)

Samad, Muna Hanim Abdul; Taib, Nooriati; Ying, Choo Siew

2017-10-01

The Uniform Building By-law 1984 of Malaysia is the legal document governing fire safety requirements in buildings. Its prescriptive nature has made the requirements out dated from the viewpoint of current performance based approach in most developed countries. The means of escape provisions is a critical requirement to safeguard occupants' safety in fire especially in public buildings. As stipulated in the UBBL 1984, the means of escape provisions includes sufficient escape routes, travel distance, protection of escape routes, etc. designated as means to allow occupants to escape within a safe period of time. This research aims at investigating the effectiveness of those provisions in public buildings during evacuation process involving massive crowd during emergencies. This research includes a scenario-based study on evacuation processes using two software i.e. PyroSim, a crowd modelling software to conduct smoke study and Pathfinder to stimulate evacuation model of building in Malaysia and Singapore as comparative study. The results show that the buildings used as case study were designed according to Malaysian UBBL 1984 and Singapore Firecode, 2013 respectively provide relative safe means of escape. The simulations of fire and smoke and coupled with simulation of evacuation have demonstrated that although there are adequate exits designated according to fire requirements, the impact of the geometry of atriums on the behavior of fire and smoke have significant effect on escape time especially for unfamiliar user of the premises.

Genetic Engineering: and the Law

ERIC Educational Resources Information Center

Australian Journal of Mental Retardation, 1977

1977-01-01

In a transcript from a radio show, Nobel Prize Winner Sir Macfarlane Burnet stresses the critical need for scientists to regulate their own activities in genetic research and cites the potential danger of creating a new form of polio which might escape. (CL)

High-Temperature Graphite/Phenolic Composite

NASA Technical Reports Server (NTRS)

Seal, Ellis C.; Bodepudi, Venu P.; Biggs, Robert W., Jr.; Cranston, John A.

1995-01-01

Graphite-fiber/phenolic-resin composite material retains relatively high strength and modulus of elasticity at temperatures as high as 1,000 degrees F. Costs only 5 to 20 percent as much as refractory materials. Fabrication composite includes curing process in which application of full autoclave pressure delayed until after phenolic resin gels. Curing process allows moisture to escape, so when composite subsequently heated in service, much less expansion of absorbed moisture and much less tendency toward delamination. Developed for nose cone of external fuel tank of Space Shuttle. Other potential aerospace applications for material include leading edges, parts of nozzles, parts of aircraft engines, and heat shields. Terrestrial and aerospace applications include structural firewalls and secondary structures in aircraft, spacecraft, and ships. Modified curing process adapted to composites of phenolic with other fiber reinforcements like glass or quartz. Useful as high-temperature circuit boards and electrical insulators.

Escape rate for nonequilibrium processes dominated by strong non-detailed balance force

NASA Astrophysics Data System (ADS)

Tang, Ying; Xu, Song; Ao, Ping

2018-02-01

Quantifying the escape rate from a meta-stable state is essential to understand a wide range of dynamical processes. Kramers' classical rate formula is the product of an exponential function of the potential barrier height and a pre-factor related to the friction coefficient. Although many applications of the rate formula focused on the exponential term, the prefactor can have a significant effect on the escape rate in certain parameter regions, such as the overdamped limit and the underdamped limit. There have been continuous interests to understand the effect of non-detailed balance on the escape rate; however, how the prefactor behaves under strong non-detailed balance force remains elusive. In this work, we find that the escape rate formula has a vanishing prefactor with decreasing friction strength under the strong non-detailed balance limit. We both obtain analytical solutions in specific examples and provide a derivation for more general cases. We further verify the result by simulations and propose a testable experimental system of a charged Brownian particle in electromagnetic field. Our study demonstrates that a special care is required to estimate the effect of prefactor on the escape rate when non-detailed balance force dominates.

MAVEN in situ measurements of photochemical escape of oxygen from Mars

NASA Astrophysics Data System (ADS)

Lillis, Robert; Deighan, Justin; Fox, Jane; Bougher, Stephen; Lee, Yuni; Cravens, Thomas; Rahmati, Ali; Mahaffy, Paul; Benna, Mehdi; Groller, Hannes; Jakosky, Bruce

2016-04-01

One of the primary goals of the MAVEN mission is to characterize rates of atmospheric escape from Mars at the present epoch and relate those escape rates to solar drivers. One of the known escape processes is photochemical escape, where a) an exothermic chemical reaction in the atmosphere results in an upward-traveling neutral particle whose velocity exceeds planetary escape velocity and b) the particle is not prevented from escaping through subsequent collisions. At Mars, photochemical escape of oxygen is expected to be a significant channel for atmospheric escape, particularly in the early solar system when extreme ultraviolet (EUV) fluxes were much higher. Thus characterizing this escape process and its variability with solar drivers is central to understanding the role escape to space has played in Mars' climate evolution. We use near-periapsis (<400 km altitude) data from three MAVEN instruments: the Langmuir Probe and Waves (LPW) instrument measures electron density and temperature, the Suprathermal And Thermal Ion Composition (STATIC) experiment measures ion temperature and the Neutral Gas and Ion Mass Spectrometer (NGIMS) measures neutral and ion densities. For each profile of in situ measurements, we make several calculations, each as a function of altitude. The first uses electron and temperatures and simulates the dissociative recombination of both O2+ and CO2+ to calculate the probability distribution for the initial energies of the resulting hot oxygen atoms. The second is a Monte Carlo hot atom transport model that takes that distribution of initial O energies and the measured neutral density profiles and calculates the probability that a hot atom born at that altitude will escape. The third takes the measured electron and ion densities and electron temperatures and calculates the production rate of hot O atoms. We then multiply together the profiles of hot atom production and escape probability to get profiles of the production rate of escaping atoms. We integrate with respect to altitude to give us the escape flux of hot oxygen atoms for that periapsis pass. We have sufficient coverage in solar zenith angle (SZA) to estimate total escape rates for two intervals with the obvious assumption that escape rates are the same at all points with the same SZA. We estimate total escape rates of 3.5-5.8 x 1025 s-1 for Ls = 289° to 319° and 1.6-2.6 x 1025 s-1 for Ls = 326° to 348°. The latter is the most directly comparable to previous model-based estimates and is roughly in line with several of them. Total photochemical loss over Mars history is not very useful to calculate from such escape fluxes derived over a limited area and under limited conditions. A thicker atmosphere and much higher solar EUV in the past may change the dynamics of escape dramatically. In the future, we intend to use 3-D Monte Carlo models of global atmospheric escape, in concert with our in situ and remote measurements, to fully characterize photochemical escape under current conditions and carefully extrapolate back in time using further simulations with new boundary conditions.

Internal combustion engine having a reactor for afterburning of unburned exhaust gas constituents

DOE Office of Scientific and Technical Information (OSTI.GOV)

Maurhoff, G.; Steinwart, J.

1974-08-07

An internal combustion engine is described which has an engine housing and a reactor for afterburning of unburned constituents in the exhaust gas. The reactor has a shell with a periphery and contains a heat-insulated, reactor chamber which is freely movable beyond the point of connection to the shell. The reactor has an inlet nozzle extending freely through the shell and connected to an outlet passage of the engine and has an outlet for escape of the exhaust gases from the reactor chamber. The inlet nozzle protrudes freely into the outlet passage, and the shell has a portion around themore » inlet nozzle in contact with the engine housing.« less

Escape from the competence state in Streptococcus mutans is governed by the bacterial population density.

PubMed

Dufour, D; Villemin, C; Perry, J A; Lévesque, C M

2016-12-01

Horizontal gene transfer through natural DNA transformation is an important evolutionary mechanism among bacteria. Transformation requires that the bacteria are physiologically competent to take and incorporate free DNA directly from the environment. Although natural genetic transformation is a remarkable feature of many naturally competent bacteria, the process is energetically expensive for the cells. Consequently, a tight control of the competence state is necessary. The objective of the present work was to help decipher the molecular mechanisms regulating the escape from the competence state in Streptococcus mutans, the principal etiological agent responsible for tooth decay in humans. Our results showed that the cessation of competence in S. mutans was abrupt, and did not involve the accumulation of a competence inhibitor nor the depletion of a competence activator in the extracellular environment. The competence state was repressed at high cell population density via concomitant repression of sigX gene encoding the master regulator of the competence regulon. Co-culture experiments performed with oral and non-oral bacteria showed that S. mutans assesses its own population density and also the microbial density of its surroundings to regulate its competence escape. Interestingly, neither the intra-species and extra-species quorum-sensing systems nor the other 13 two-component regulatory systems identified in S. mutans were involved in the cell-density-dependent escape of the competence state. Altogether, our results suggest a complex mechanism regulating the competence shut-off involving cell-density-dependent repression of sigX through an as yet undefined system, and possibly SigX protein stability. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

13. View inside Building 802, the "Sleeping Quarters" looking down ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. View inside Building 802, the "Sleeping Quarters" looking down the west side, bunks on the left, escape hatch at rear, facing south. - Naval Air Station Fallon, 100-man Fallout Shelter, 800 Complex, off Carson Road near intersection of Pasture & Berney Roads, Fallon, Churchill County, NV

A technician works adjacent to the Apollo 11 spacecraft atop the white room.

NASA Technical Reports Server (NTRS)

1969-01-01

A technician can be seen working atop the white room across from the escape tower of the Apollo 11 spacecraft a few days prior to the launch of the Saturn V moon rocket. The towering 363-foot Saturn V was a multi-stage, multi-engine launch vehicle standing taller than the Statue of Liberty. Altogether, the Saturn V engines produced as much power as 85 Hoover Dams

Expedition 19 Soyuz Assembly

NASA Image and Video Library

2009-06-09

Engineers assemble the Soyuz TMA-14 spacecraft, escape tower and all three stages Monday, March 23, 2009 at the Baikonur Cosmodrome in Kazakhstan. The Soyuz is scheduled to launch the crew of Expedition 19 and a spaceflight participant on March 26, 2009. Photo Credit: (NASA/Bill Ingalls)

From cradle-to-grave at the nanoscale: gaps in U.S. regulatory oversight along the nanomaterial life cycle.

PubMed

Beaudrie, Christian E H; Kandlikar, Milind; Satterfield, Terre

2013-06-04

Engineered nanomaterials (ENMs) promise great benefits for society, yet our knowledge of potential risks and best practices for regulation are still in their infancy. Toward the end of better practices, this paper analyzes U.S. federal environmental, health, and safety (EHS) regulations using a life cycle framework. It evaluates their adequacy as applied to ENMs to identify gaps through which emerging nanomaterials may escape regulation from initial production to end-of-life. High scientific uncertainty, a lack of EHS and product data, inappropriately designed exemptions and thresholds, and limited agency resources are a challenge to both the applicability and adequacy of current regulations. The result is that some forms of engineered nanomaterials may escape federal oversight and rigorous risk review at one or more stages along their life cycle, with the largest gaps occurring at the postmarket stages, and at points of ENM release to the environment. Oversight can be improved through pending regulatory reforms, increased research and development for the monitoring, control, and analysis of environmental and end-of-life releases, introduction of periodic re-evaluation of ENM risks, and fostering a "bottom-up" stewardship approach to the responsible management of risks from engineered nanomaterials.

PROCESS WATER BUILDING, TRA605. CONTROL PANEL SUPPLIES STATUS INDICATORS. CARD ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PROCESS WATER BUILDING, TRA-605. CONTROL PANEL SUPPLIES STATUS INDICATORS. CARD IN LOWER RIGHT WAS INSERTED BY INL PHOTOGRAPHER TO COVER AN OBSOLETE SECURITY RESTRICTION ON ORIGINAL NEGATIVE. INL NEGATIVE NO. 4219. Unknown Photographer, 2/13/1952 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

Venus, Earth, Mars: Comparative ion escape caused by the interaction with the solar wind

NASA Astrophysics Data System (ADS)

Barabash, Stas

For the solar system planets the non-thermal atmospheric escape exceeds by far the Jean escape for particles heavier than helium. In this talk we consider only ion escape and compare the total ion escape rates for Venus, Earth, and Mars caused by the interaction with the solar wind. We review the most recent data on the escape rates based on measurements from Mars Express, Venus Express, and Cluster. The comparison of the available numbers show that despite large differences in the atmospheric masses between these three planets (a factor of 100 -200), different types of the interactions with the solar wind (magnetized and non-magnetized obstacles), the escape rates for Mars, Venus, and the Earth are within the range 1024 - 1025 s-1 . Surprisingly, the expected shielding of the Earth atmosphere by the intrinsic magnetic field is not as efficient as one may think. The reason for this is the non-thermal escape caused by the solar wind interaction is a energy -limited process. Indeed, normalizing the escape rates to the planet-dependent escape energy and power available in the solar wind results in the normalized escape rates deferring only on a factor between three planets. The larger Earth's magnetosphere intercepts and tunnels down to the ionosphere more energy from the solar wind than more compact interaction regions of non-magnetized planets.

Expedition 19 Soyuz Assembly

NASA Image and Video Library

2009-03-22

Russian engineers attach the escape tower to the Soyuz TMA-14 spacecraft and boosters Monday, March 23, 2009 at the Baikonur Cosmodrome in Kazakhstan. The Soyuz is scheduled to launch the crew of Expedition 19 and a spaceflight participant on March 26, 2009. Photo Credit: (NASA/Bill Ingalls)

On the temperature prediction in a fire escape passage

NASA Astrophysics Data System (ADS)

Casano, G.; Piva, S.

2017-11-01

Fire safety engineering requires a detailed understanding of fire behaviour and of its effects on structures and people. Many factors may condition the fire scenario, as for example, heat transfer between the flame and the boundary structures. Currently advanced numerical codes for the prediction of the fire behaviour are available. However, these solutions often require heavy calculations and long times. In this context analytical solutions can be useful for a fast analysis of simplified schematizations. After that, it is more effective the final utilization of the advanced fire codes. In this contribution, the temperature in a fire escape passage, separated with a thermally resistant wall from a fire room, is analysed. The escape space is included in a building where the neighbouring rooms are at a constant undisturbed temperature. The presence of the neighbouring rooms is considered with an equivalent heat transfer coefficient, in a boundary condition of the third type. An analytical solution is used to predict the temperature distribution during the fire. It allows to obtain useful information on the temperature reached in the escape area in contact with a burning room; it is useful also for a fast choice of the thermal characteristics of a firewall.

Historical Footage of John Glenn Friendship 7

NASA Technical Reports Server (NTRS)

1962-01-01

The Friendship mission launch on the 20th day of February marked the first time that an American attempts to orbit the Earth. Historical footage of John Glenn's suit up, ride out to the launch pad, countdown, liftoff, booster engine cutoff, and separation of the booster engine escape tower is shown. Views of the Earth, Glenn's manual control of the electrical fly-by wire system, and the recovery of the landing vehicle from the ocean are presented.

[AVIAN RECOMBINANT VIRUS H5N1 INFLUENZA (A/VIETNAM/1203/04) AND ITS ESCAPE-MUTANT m13(13) INDUCE EARLY SIGNALING REACTIONS OF THE IMMUNITY IN HUMAN LYMPHOCYTES].

PubMed

Sokolova, T M; Poloskov, V V; Shuvalov, A N; Rudneva, I A; Ershov, F I

2016-01-01

The innate immune receptors TLR4, TLR7, TLR8, and RIG1 recognized the structures of the influenza viruses in human lymphocytes and were activated by the recombinant avian influenza virus A/Vietnam/1203/04 and its escape-mutant m13(13) during early period of interaction. The stimulated levels are not connected with viral reproduction. Donor cells with the low constitutive immune receptors gene expression levels showed higher stimulation. Inflammation virus effects resulted in. increasing production of TNF-alpha and IFN-gamma by lymphocytes. Signaling gene reactions of the parent and mutant viruses endosomal as well as cytoplasmic receptors are very similar. The mutant virus A/Vietnam/1203/04 (HA S145F), stimulated an increase in the transcription level of the membrane receptor gene TLR4 and a decrease in the level of activation of TNF-alpha gene. Further studies of natural influenza virus isolates are necessary to estimate the role of HA antigenic changes on immune reactions in humans.

Noise induced escape from a nonhyperbolic chaotic attractor of a periodically driven nonlinear oscillator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Zhen, E-mail: czkillua@icloud.com, E-mail: xbliu@nuaa.edu.cn; Li, Yang; Liu, Xianbin, E-mail: czkillua@icloud.com, E-mail: xbliu@nuaa.edu.cn

2016-06-15

Noise induced escape from the domain of attraction of a nonhyperbolic chaotic attractor in a periodically excited nonlinear oscillator is investigated. The general mechanism of the escape in the weak noise limit is studied in the continuous case, and the fluctuational path is obtained by statistical analysis. Selecting the primary homoclinic tangency as the initial condition, the action plot is presented by parametrizing the set of escape trajectories and the global minimum gives rise to the optimal path. Results of both methods show good agreements. The entire process of escape is discussed in detail step by step using the fluctuationalmore » force. A structure of hierarchical heteroclinic crossings of stable and unstable manifolds of saddle cycles is found, and the escape is observed to take place through successive jumps through this deterministic hierarchical structure.« less

Neural circuits underlying visually evoked escapes in larval zebrafish

PubMed Central

Dunn, Timothy W.; Gebhardt, Christoph; Naumann, Eva A.; Riegler, Clemens; Ahrens, Misha B.; Engert, Florian; Del Bene, Filippo

2015-01-01

SUMMARY Escape behaviors deliver organisms away from imminent catastrophe. Here, we characterize behavioral responses of freely swimming larval zebrafish to looming visual stimuli simulating predators. We report that the visual system alone can recruit lateralized, rapid escape motor programs, similar to those elicited by mechanosensory modalities. Two-photon calcium imaging of retino-recipient midbrain regions isolated the optic tectum as an important center processing looming stimuli, with ensemble activity encoding the critical image size determining escape latency. Furthermore, we describe activity in retinal ganglion cell terminals and superficial inhibitory interneurons in the tectum during looming and propose a model for how temporal dynamics in tectal periventricular neurons might arise from computations between these two fundamental constituents. Finally, laser ablations of hindbrain circuitry confirmed that visual and mechanosensory modalities share the same premotor output network. Together, we establish a circuit for the processing of aversive stimuli in the context of an innate visual behavior. PMID:26804997

Interculturalism and the Pendulum of Identity

ERIC Educational Resources Information Center

Svirsky, Marcelo; Mor-Sommerfeld, Aura

2012-01-01

This article offers a critical appraisal of the role played by cultural identity in intercultural bilingual Arabic-Hebrew schools in Israel. While engineered as oases of interculturalism amidst a life of ethnic segregation, such schools ultimately confront serious difficulties in escaping the constraints of identity politics and representation.…

THERMALLY DRIVEN ATMOSPHERIC ESCAPE: TRANSITION FROM HYDRODYNAMIC TO JEANS ESCAPE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Volkov, Alexey N.; Johnson, Robert E.; Tucker, Orenthal J.

2011-03-10

Thermally driven escape from planetary atmospheres changes in nature from an organized outflow (hydrodynamic escape) to escape on a molecule-by-molecule basis (Jeans escape) with increasing Jeans parameter, {lambda}, the ratio of the gravitational to thermal energy of the atmospheric molecules. This change is described here for the first time using the direct simulation Monte Carlo method. When heating is predominantly below the lower boundary of the simulation region, R{sub 0}, and well below the exobase of a single-component atmosphere, the nature of the escape process changes over a surprisingly narrow range of Jeans parameters, {lambda}{sub 0}, evaluated at R{sub 0}.more » For an atomic gas, the transition occurs over {lambda}{sub 0} {approx} 2-3, where the lower bound, {lambda}{sub 0} {approx} 2.1, corresponds to the upper limit for isentropic, supersonic outflow. For {lambda}{sub 0} > 3 escape occurs on a molecule-by-molecule basis and we show that, contrary to earlier suggestions, for {lambda}{sub 0} > {approx}6 the escape rate does not deviate significantly from the familiar Jeans rate. In a gas composed of diatomic molecules, the transition shifts to {lambda}{sub 0} {approx} 2.4-3.6 and at {lambda}{sub 0} > {approx}4 the escape rate increases a few tens of percent over that for the monatomic gas. Scaling by the Jeans parameter and the Knudsen number, these results can be applied to thermally induced escape of the major species from solar and extrasolar planets.« less

Influence of throat configuration and fish density on escapement of channel catfish from hoop nets

USGS Publications Warehouse

Porath, Mark T.; Pape, Larry D.; Richters, Lindsey K.

2011-01-01

In recent years, several state agencies have adopted the use of baited, tandemset hoop nets to assess lentic channel catfish Ictalurus punctatus populations. Some level of escapement from the net is expected because an opening exists in each throat of the net, although factors influencing rates of escapement from hoop nets have not been quantified. We conducted experiments to quantify rates of escapement and to determine the influence of throat configuration and fish density within the net on escapement rates. An initial experiment to determine the rate of escapement from each net compartment utilized individually tagged channel catfish placed within the entrance (between the two throats) and cod (within the second throat) compartments of a single hoop net for overnight sets. From this experiment, the mean rate (±SE) of channel catfish escaping was 4.2% (±1.5) from the cod (cod throat was additionally restricted from the traditionally manufactured product), and 74% (±4.2) from the entrance compartments. In a subsequent experiment, channel catfish were placed only in the cod compartment with different throat configurations (restricted or unrestricted) and at two densities (low [6 fish per net] and high [60 fish per net]) for overnight sets to determine the influence of fish density and throat configuration on escapement rates. Escapement rates between throat configurations were doubled at low fish density (13.3 ± 5.4% restricted versus 26.7 ± 5.6% unrestricted) and tripled at high fish density (14.3 ± 4.9% restricted versus 51.9 ± 5.0% unrestricted). These results suggest that retention efficiency is high from cod compartments with restricted throat entrances. However, managers and researchers need to be aware that modification to the cod throats (restrictions) is needed for hoop nets ordered from manufacturers. Managers need to be consistent in their use and reporting of cod end throat configurations when using this gear.

CrossTalk: The Journal of Defense Software Engineering. Volume 18, Number 9

DTIC Science & Technology

2005-09-01

2004. 12. Humphrey , Watts . Introduction to the Personal Software Process SM. Addison- Wesley 1997. 13. Humphrey , Watts . Introduction to the Team...Personal Software ProcessSM (PSPSM)is a software development process orig- inated by Watts Humphrey at the Software Engineering Institute (SEI) in the...meets its commitments and bring a sense of control and predictability into an apparently chaotic project.u References 1. Humphrey , Watts . Coaching

Kramers problem in evolutionary strategies

NASA Astrophysics Data System (ADS)

Dunkel, J.; Ebeling, W.; Schimansky-Geier, L.; Hänggi, P.

2003-06-01

We calculate the escape rates of different dynamical processes for the case of a one-dimensional symmetric double-well potential. In particular, we compare the escape rates of a Smoluchowski process, i.e., a corresponding overdamped Brownian motion dynamics in a metastable potential landscape, with the escape rates obtained for a biologically motivated model known as the Fisher-Eigen process. The main difference between the two models is that the dynamics of the Smoluchowski process is determined by local quantities, whereas the Fisher-Eigen process is based on a global coupling (nonlocal interaction). If considered in the context of numerical optimization algorithms, both processes can be interpreted as archetypes of physically or biologically inspired evolutionary strategies. In this sense, the results discussed in this work are utile in order to evaluate the efficiency of such strategies with regard to the problem of surmounting various barriers. We find that a combination of both scenarios, starting with the Fisher-Eigen strategy, provides a most effective evolutionary strategy.

Radiochemical data collected on events from which radioactivity escaped beyond the borders of the Nevada test range complex. [NONE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hicks, H.G.

1981-02-12

This report identifies all nuclear events in Nevada that are known to have sent radioactivity beyond the borders of the test range complex. There have been 177 such tests, representing seven different types: nuclear detonations in the atmosphere, nuclear excavation events, nuclear safety events, underground nuclear events that inadvertently seeped or vented to the atmosphere, dispersion of plutonium and/or uranium by chemical high explosives, nuclear rocket engine tests, and nuclear ramjet engine tests. The source term for each of these events is given, together with the data base from which it was derived (except where the data are classified). Themore » computer programs used for organizing and processing the data base and calculating radionuclide production are described and included, together with the input and output data and details of the calculations. This is the basic formation needed to make computer modeling studies of the fallout from any of these 177 events.« less

Hybrid biosynthetic gene therapy vector development and dual engineering capacity.

PubMed

Jones, Charles H; Ravikrishnan, Anitha; Chen, Mingfu; Reddinger, Ryan; Kamal Ahmadi, Mahmoud; Rane, Snehal; Hakansson, Anders P; Pfeifer, Blaine A

2014-08-26

Genetic vaccines offer a treatment opportunity based upon successful gene delivery to specific immune cell modulators. Driving the process is the vector chosen for gene cargo packaging and subsequent delivery to antigen-presenting cells (APCs) capable of triggering an immune cascade. As such, the delivery process must successfully navigate a series of requirements and obstacles associated with the chosen vector and target cell. In this work, we present the development and assessment of a hybrid gene delivery vector containing biological and biomaterial components. Each component was chosen to design and engineer gene delivery separately in a complimentary and fundamentally distinct fashion. A bacterial (Escherichia coli) inner core and a biomaterial [poly(beta-amino ester)]-coated outer surface allowed the simultaneous application of molecular biology and polymer chemistry to address barriers associated with APC gene delivery, which include cellular uptake and internalization, phagosomal escape, and intracellular cargo concentration. The approach combined and synergized normally disparate vector properties and tools, resulting in increased in vitro gene delivery beyond individual vector components or commercially available transfection agents. Furthermore, the hybrid device demonstrated a strong, efficient, and safe in vivo humoral immune response compared with traditional forms of antigen delivery. In summary, the flexibility, diversity, and potential of the hybrid design were developed and featured in this work as a platform for multivariate engineering at the vector and cellular scales for new applications in gene delivery immunotherapy.

First-passage and escape problems in the Feller process

NASA Astrophysics Data System (ADS)

Masoliver, Jaume; Perelló, Josep

2012-10-01

The Feller process is an one-dimensional diffusion process with linear drift and state-dependent diffusion coefficient vanishing at the origin. The process is positive definite and it is this property along with its linear character that have made Feller process a convenient candidate for the modeling of a number of phenomena ranging from single-neuron firing to volatility of financial assets. While general properties of the process have long been well known, less known are properties related to level crossing such as the first-passage and the escape problems. In this work we thoroughly address these questions.

Trajectory Optimization of a Bimodal Nuclear Powered Spacecraft to Mars

DTIC Science & Technology

1990-05-29

velocity M = initial mass of spacecraft o m= ion fuel expulsion rate (constant) 0 = thrust direction angle = gravitational constant of Sun AVto t...total velocity change possible for the impulsive engines AV1 = velocity change for Earth escape AV2 = velocity change for Mars capture AVto t = AV + AV

49 CFR 830.5 - Immediate notification.

Code of Federal Regulations, 2011 CFR

2011-10-01

... any internal turbine engine component that results in the escape of debris other than out the exhaust... utilized. (8) Release of all or a portion of a propeller blade from an aircraft, excluding release caused... airspace. (11) Damage to helicopter tail or main rotor blades, including ground damage, that requires major...

49 CFR 830.5 - Immediate notification.

Code of Federal Regulations, 2014 CFR

2014-10-01

... any internal turbine engine component that results in the escape of debris other than out the exhaust... utilized. (8) Release of all or a portion of a propeller blade from an aircraft, excluding release caused... airspace. (11) Damage to helicopter tail or main rotor blades, including ground damage, that requires major...

49 CFR 830.5 - Immediate notification.

Code of Federal Regulations, 2012 CFR

2012-10-01

... any internal turbine engine component that results in the escape of debris other than out the exhaust... utilized. (8) Release of all or a portion of a propeller blade from an aircraft, excluding release caused... airspace. (11) Damage to helicopter tail or main rotor blades, including ground damage, that requires major...

49 CFR 830.5 - Immediate notification.

Code of Federal Regulations, 2013 CFR

2013-10-01

... any internal turbine engine component that results in the escape of debris other than out the exhaust... utilized. (8) Release of all or a portion of a propeller blade from an aircraft, excluding release caused... airspace. (11) Damage to helicopter tail or main rotor blades, including ground damage, that requires major...

Expedition 19 Soyuz Assembly

NASA Image and Video Library

2009-03-22

Russian engineers prepare the escape tower prior to attaching it to the Soyuz TMA-14 spacecraft and boosters Monday, March 23, 2009 at the Baikonur Cosmodrome in Kazakhstan. The Soyuz is scheduled to launch the crew of Expedition 19 and a spaceflight participant on March 26, 2009. Photo Credit: (NASA/Bill Ingalls)

Constellation

NASA Image and Video Library

2008-02-15

SHOWN IS A CONCEPT IMAGE OF THE ARES V EARTH DEPARTURE STAGE AND LUNAR SURFACE ACCESS MODULE DOCKED WITH THE ORION CREW EXPLORATION VEHICLE IN EARTH ORBIT. THE DEPARTURE STAGE, POWERED BY A J-2X ENGINE, IS NEEDED TO ESCAPE EARTH'S GRAVITY AND SEND THE CREW VEHICLE AND LUNAR MODULE ON THEIR JOURNEY TO THE MOON.

Deep Space Transportation System Using the Sun-Earth L2 Point

NASA Technical Reports Server (NTRS)

Matsumoto, Michihiro

2007-01-01

Recently, various kinds of planetary explorations have become more feasible, taking the advantage of low thrust propulsion means such as ion engines that have come into practical use. The field of space activity has now been expanded even to the rim of the outer solar system. In this context, the Japan Aerospace Exploration Agency (JAXA) has started investigating a Deep Space Port built at the L2 Lagrange point in the Sun-Earth system. For the purpose of making the deep space port practically useful, there is a need to establish a method to making spaceship depart and return from/to the port. This paper first discusses the escape maneuvers originating from the L2 point under the restricted three-body problem. Impulsive maneuvers from the L2 point are extensively studied here, and using the results, optimal low-thrust escape strategies are synthesized. Furthermore, this paper proposes the optimal escape and acceleration maneuvers schemes using Electric Delta-V Earth Gravity Assist (EDVEGA) technique.

A NASA technician paints NASA's first Orion full-scale abort flight test crew module.

NASA Image and Video Library

2008-03-31

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

Sporting a fresh paint job, NASA's first Orion full-scale abort flight test crew module awaits avionics and other equipment installation.

NASA Image and Video Library

2008-04-01

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

Different types of avoidance behavior in rats produce dissociable post-training changes in sleep.

PubMed

Fogel, Stuart M; Smith, Carlyle T; Higginson, Caitlin D; Beninger, Richard J

2011-02-01

Avoidance learning affects post-training sleep, and post-training sleep deprivation impairs performance. However, not all rats learn to make avoidance responses, and some rats fail to escape; a definitive behavior of learned helplessness, a model of depression. This study investigated the changes in sleep associated with different behaviors adopted following avoidance training. Rats (n=53) were trained for 100 trials over 2 days (50 trials/day), followed by 23-24 h of post-training polysomnography, then re-tested (25 trials). At re-test, rats were categorized into: 1) Active Avoiders (AA; n=22), 2), Non-learning (NL; n=21), or 3) Escape Failures (EF; n=10). AA rats increased avoidances over days, whereas the NL and EF groups did not. EF rats increased escape failures over days, whereas the NL and AA rats did not. EF rats had increased rapid eye movement (REM) sleep in the first 4h on training day 1. They also had increased non-REM sleep in the first 4h and last 4h on both training days. AA rats had increased REM sleep 13-20 h post-training. The type of behavioral strategy adopted throughout training is associated with a unique pattern of changes in post-training sleep. Training-dependent changes in post-acquisition sleep may reflect distinct processes involved in the consolidation of these different memory traces. Copyright © 2010 Elsevier Inc. All rights reserved.

Extended Operation of Turbojet Engine with Pentaborane

NASA Technical Reports Server (NTRS)

Useller, James W; Jones, William L

1957-01-01

A full-scale turbojet engine was operated with pentaborane fuel continuously for 22 minutes at conditions simulating flight at a Mach number of 0.8 at an altitude of 50,000 feet. This period of operation is approximately three times longer than previously reported operation times. Although the specific fuel consumption was reduced from 1.3 with JP-4 fuel to 0.98 with pentaborane, a 13.2-percent reduction in net thrust was also encountered. A portion of this thrust loss is potentially recoverable with proper design of the engine components. The boron oxide deposition and erosion processes within the engine approached an equilibrium condition after approximately 22 minutes of operation with pentaborane.

Non-thermal escape rates of atmospheric H and D from Mars using MAVEN data

NASA Astrophysics Data System (ADS)

Gacesa, M.; Zahnle, K. J.

2017-12-01

Geological evidence suggests that an ocean of liquid water existed on Mars until at least middle to late Noachian era (4.1 to 3.8 Ga) and possibly, at least episodically, as late as Hesperian. Between 67% and 87% of the total primordial amount of water, equal to about 70 to 110 meters equivalent (spread over the entire Mars' surface), is believed to have escape to space, while about 35 meters remains on or beneath the surface as water ice. Establishing better constraints on these numbers and identifying the responsible atmospheric loss processes remains the major objective of NASA's Mars Atmosphere and Volatile EvolutioN (MAVEN) mission. The ratio of atmospheric Deuterium and Hydrogen (D/H) on Mars is one of the best indicators of water loss to space. While majority of H and D escape through thermal Jeans escape, up to 10% of D can escape to space via non-thermal mechanisms, such as collisions with superthermal neutral atoms. In this study, we present new estimates of non-thermal escape rates of light molecules of interest to the water evolution, including H2, HD, OH, and OD, based on recent measurements of atmospheric density and temperature profiles by MAVEN. The escape mechanisms considered include photochemical sources of hot O, as well as collisions with energetic neutral atoms produced in charge-exchange of solar wind ions with atmospheric gases1,2. Energy transport and escape rates are modeled using quantum reactive scattering formalism3 and seasonal variations are illustrated. Finally, a simple estimate of the role of the non-thermal escape mechanisms in previous eras is given. We conclude that D escape rates can be affected by the non-thermal processes with consequences on the estimates of primordial water inventory based on the D/H ratio. [1] N. Lewkow and V. Kharchenko, Astroph. J., 790, 98 (2014) [2] M. Gacesa, N. Lewkow, V. Kharchenko, Icarus 284, 90 (2017) [3] M. Gacesa and V. Kharchenko, Geophys. Res. Lett., 39, L10203 (2012)

Entangled Dynamics in Macroscopic Quantum Tunneling of Bose-Einstein Condensates

NASA Astrophysics Data System (ADS)

Alcala, Diego A.; Glick, Joseph A.; Carr, Lincoln D.

2017-05-01

Tunneling of a quasibound state is a nonsmooth process in the entangled many-body case. Using time-evolving block decimation, we show that repulsive (attractive) interactions speed up (slow down) tunneling. While the escape time scales exponentially with small interactions, the maximization time of the von Neumann entanglement entropy between the remaining quasibound and escaped atoms scales quadratically. Stronger interactions require higher-order corrections. Entanglement entropy is maximized when about half the atoms have escaped.

CMMI(Registered) for Acquisition, Version 1.3. CMMI-ACQ, V1.3

DTIC Science & Technology

2010-11-01

and Software Engineering – System Life Cycle Processes [ ISO 2008b] ISO /IEC 27001 :2005 Information technology – Security techniques – Information...International Organization for Standardization and International Electrotechnical Commission. ISO /IEC 27001 Information Technology – Security Techniques...International Organization for Standardization/International Electrotechnical Commission ( ISO /IEC) body of standards. CMMs focus on improving processes

The Metalloprotease Mpl Supports Listeria monocytogenes Dissemination through Resolution of Membrane Protrusions into Vacuoles

PubMed Central

Alvarez, Diego E.

2016-01-01

Listeria monocytogenes is an intracellular pathogen that disseminates within the intestinal epithelium through acquisition of actin-based motility and formation of plasma membrane protrusions that project into adjacent cells. The resolution of membrane protrusions into vacuoles from which the pathogen escapes results in bacterial spread from cell to cell. This dissemination process relies on the mlp-actA-plcB operon, which encodes ActA, a bacterial nucleation-promoting factor that mediates actin-based motility, and PlcB, a phospholipase that mediates vacuole escape. Here we investigated the role of the metalloprotease Mpl in the dissemination process. In agreement with previous findings showing that Mpl is required for PlcB activation, infection of epithelial cells with the ΔplcB or Δmpl strains resulted in the formation of small infection foci. As expected, the ΔplcB strain displayed a strong defect in vacuole escape. However, the Δmpl strain showed an unexpected defect in the resolution of protrusions into vacuoles, in addition to the expected but mild defect in vacuole escape. The Δmpl strain displayed increased levels of ActA on the bacterial surface in protrusions. We mapped an Mpl-dependent processing site in ActA between amino acid residues 207 to 238. Similar to the Δmpl strain, the ΔactA207–238 strain displayed increased levels of ActA on the bacterial surface in protrusions. Although the ΔactA207–238 strain displayed wild-type actin-based motility, it formed small infection foci and failed to resolve protrusions into vacuoles. We propose that, in addition to its role in PlcB processing and vacuole escape, the metalloprotease Mpl is required for ActA processing and protrusion resolution. PMID:27068088

The Metalloprotease Mpl Supports Listeria monocytogenes Dissemination through Resolution of Membrane Protrusions into Vacuoles.

PubMed

Alvarez, Diego E; Agaisse, Hervé

2016-06-01

Listeria monocytogenes is an intracellular pathogen that disseminates within the intestinal epithelium through acquisition of actin-based motility and formation of plasma membrane protrusions that project into adjacent cells. The resolution of membrane protrusions into vacuoles from which the pathogen escapes results in bacterial spread from cell to cell. This dissemination process relies on the mlp-actA-plcB operon, which encodes ActA, a bacterial nucleation-promoting factor that mediates actin-based motility, and PlcB, a phospholipase that mediates vacuole escape. Here we investigated the role of the metalloprotease Mpl in the dissemination process. In agreement with previous findings showing that Mpl is required for PlcB activation, infection of epithelial cells with the ΔplcB or Δmpl strains resulted in the formation of small infection foci. As expected, the ΔplcB strain displayed a strong defect in vacuole escape. However, the Δmpl strain showed an unexpected defect in the resolution of protrusions into vacuoles, in addition to the expected but mild defect in vacuole escape. The Δmpl strain displayed increased levels of ActA on the bacterial surface in protrusions. We mapped an Mpl-dependent processing site in ActA between amino acid residues 207 to 238. Similar to the Δmpl strain, the ΔactA207-238 strain displayed increased levels of ActA on the bacterial surface in protrusions. Although the ΔactA207-238 strain displayed wild-type actin-based motility, it formed small infection foci and failed to resolve protrusions into vacuoles. We propose that, in addition to its role in PlcB processing and vacuole escape, the metalloprotease Mpl is required for ActA processing and protrusion resolution. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

DR-induced escape of O and C from early Mars

NASA Astrophysics Data System (ADS)

Zhao, Jinjin; Tian, Feng; Ni, Yufang; Huang, Xiaomeng

2017-03-01

Energetic particles produced in Dissociative recombination (DR) reactions could escape planets with low gravity, such as Mars, if they could overcome collisions with the surrounding background gases. In this work, a 3-D Monte Carlo model is developed to study these photochemical escape processes on early Mars. Although the DR reaction rates of O2+, CO2+, and CO+ increase monotonically with solar soft X-ray and extreme ultraviolet (XUV) flux, the peak of the calculated DR-induced escape rates of O is near 3 × XUV, and the DR-induced escape rates of C increase with XUV until 10 × XUV. The non-monotonic behavior can be explained by the increased column densities of background species in high XUV conditions, which can deflect energetic particles through collisions more efficiently. At 20 × XUV, CO+ DR is the main source of escaping O and C, and the escape of secondary particles could contribute to 30∼40% and 10% of the total escape of O and C respectively. The time-integrated DR-induced escape of O and C is equivalent to 1 m of H2O and 20 mbar of CO2 escaping early Mars since 4.5 billion years ago. The accumulated CO2 loss is much lower than what's needed to explain the carbon isotopic ratios on Mars and much lower than the total CO2 needed to warm up early Mars. If more vigorous escape mechanisms were absent on early Mars, substantial inventories of volatiles have not been detected yet.

Hot oxygen escape from Mars: Simple scaling with solar EUV irradiance

NASA Astrophysics Data System (ADS)

Cravens, T. E.; Rahmati, A.; Fox, Jane L.; Lillis, R.; Bougher, S.; Luhmann, J.; Sakai, S.; Deighan, J.; Lee, Yuni; Combi, M.; Jakosky, B.

2017-01-01

The evolution of the atmosphere of Mars and the loss of volatiles over the lifetime of the solar system is a key topic in planetary science. An important loss process for atomic species, such as oxygen, is ionospheric photochemical escape. Dissociative recombination of O2+ ions (the major ion species) produces fast oxygen atoms, some of which can escape from the planet. Many theoretical hot O models have been constructed over the years, although a number of uncertainties are present in these models, particularly concerning the elastic cross sections of O atoms with CO2. Recently, the Mars Atmosphere and Volatile Evolution mission has been rapidly improving our understanding of the upper atmosphere and ionosphere of Mars and its interaction with the external environment (e.g., solar wind), allowing a new assessment of this important loss process. The purpose of the current paper is to take a simple analytical approach to the oxygen escape problem in order to (1) study the role that variations in solar radiation or solar wind fluxes could have on escape in a transparent fashion and (2) isolate the effects of uncertainties in oxygen cross sections on the derived oxygen escape rates. In agreement with several more elaborate numerical models, we find that the escape flux is directly proportional to the incident solar extreme ultraviolet irradiance and is inversely proportional to the backscatter elastic cross section. The amount of O lost due to ion transport in the topside ionosphere is found to be about 5-10% of the total.

Microbiological Spoilage of Dairy Products

NASA Astrophysics Data System (ADS)

Ledenbach, Loralyn H.; Marshall, Robert T.

The wide array of available dairy foods challenges the microbiologist, engineer, and technologist to find the best ways to prevent the entry of microorganisms, destroy those that do get in along with their enzymes, and prevent the growth and activities of those that escape processing treatments. Troublesome spoilage microorganisms include aerobic psychrotrophic Gram-negative bacteria, yeasts, molds, heterofermentative lactobacilli, and spore-forming bacteria. Psychrotrophic bacteria can produce large amounts of extracellular hydrolytic enzymes, and the extent of recontamination of pasteurized fluid milk products with these bacteria is a major determinant of their shelf life. Fungal spoilage of dairy foods is manifested by the presence of a wide variety of metabolic by-products, causing off-odors and flavors, in addition to visible changes in color or texture.

Enhanced Endosomal Escape by Light-Fueled Liquid-Metal Transformer.

PubMed

Lu, Yue; Lin, Yiliang; Chen, Zhaowei; Hu, Quanyin; Liu, Yang; Yu, Shuangjiang; Gao, Wei; Dickey, Michael D; Gu, Zhen

2017-04-12

Effective endosomal escape remains as the "holy grail" for endocytosis-based intracellular drug delivery. To date, most of the endosomal escape strategies rely on small molecules, cationic polymers, or pore-forming proteins, which are often limited by the systemic toxicity and lack of specificity. We describe here a light-fueled liquid-metal transformer for effective endosomal escape-facilitated cargo delivery via a chemical-mechanical process. The nanoscale transformer can be prepared by a simple approach of sonicating a low-toxicity liquid-metal. When coated with graphene quantum dots (GQDs), the resulting nanospheres demonstrate the ability to absorb and convert photoenergy to drive the simultaneous phase separation and morphological transformation of the inner liquid-metal core. The morphological transformation from nanospheres to hollow nanorods with a remarkable change of aspect ratio can physically disrupt the endosomal membrane to promote endosomal escape of payloads. This metal-based nanotransformer equipped with GQDs provides a new strategy for facilitating effective endosomal escape to achieve spatiotemporally controlled drug delivery with enhanced efficacy.

Comparing land use regression and dispersion modelling to assess residential exposure to ambient air pollution for epidemiological studies.

PubMed

de Hoogh, Kees; Korek, Michal; Vienneau, Danielle; Keuken, Menno; Kukkonen, Jaakko; Nieuwenhuijsen, Mark J; Badaloni, Chiara; Beelen, Rob; Bolignano, Andrea; Cesaroni, Giulia; Pradas, Marta Cirach; Cyrys, Josef; Douros, John; Eeftens, Marloes; Forastiere, Francesco; Forsberg, Bertil; Fuks, Kateryna; Gehring, Ulrike; Gryparis, Alexandros; Gulliver, John; Hansell, Anna L; Hoffmann, Barbara; Johansson, Christer; Jonkers, Sander; Kangas, Leena; Katsouyanni, Klea; Künzli, Nino; Lanki, Timo; Memmesheimer, Michael; Moussiopoulos, Nicolas; Modig, Lars; Pershagen, Göran; Probst-Hensch, Nicole; Schindler, Christian; Schikowski, Tamara; Sugiri, Dorothee; Teixidó, Oriol; Tsai, Ming-Yi; Yli-Tuomi, Tarja; Brunekreef, Bert; Hoek, Gerard; Bellander, Tom

2014-12-01

Land-use regression (LUR) and dispersion models (DM) are commonly used for estimating individual air pollution exposure in population studies. Few comparisons have however been made of the performance of these methods. Within the European Study of Cohorts for Air Pollution Effects (ESCAPE) we explored the differences between LUR and DM estimates for NO2, PM10 and PM2.5. The ESCAPE study developed LUR models for outdoor air pollution levels based on a harmonised monitoring campaign. In thirteen ESCAPE study areas we further applied dispersion models. We compared LUR and DM estimates at the residential addresses of participants in 13 cohorts for NO2; 7 for PM10 and 4 for PM2.5. Additionally, we compared the DM estimates with measured concentrations at the 20-40 ESCAPE monitoring sites in each area. The median Pearson R (range) correlation coefficients between LUR and DM estimates for the annual average concentrations of NO2, PM10 and PM2.5 were 0.75 (0.19-0.89), 0.39 (0.23-0.66) and 0.29 (0.22-0.81) for 112,971 (13 study areas), 69,591 (7) and 28,519 (4) addresses respectively. The median Pearson R correlation coefficients (range) between DM estimates and ESCAPE measurements were of 0.74 (0.09-0.86) for NO2; 0.58 (0.36-0.88) for PM10 and 0.58 (0.39-0.66) for PM2.5. LUR and dispersion model estimates correlated on average well for NO2 but only moderately for PM10 and PM2.5, with large variability across areas. DM predicted a moderate to large proportion of the measured variation for NO2 but less for PM10 and PM2.5. Copyright © 2014 Elsevier Ltd. All rights reserved.

A Substantial Plume of Escaping Planetary Ions in the MSE Northern Hemisphere Observed by MAVEN

NASA Astrophysics Data System (ADS)

Dong, Y.; Fang, X.; Brain, D. A.; McFadden, J. P.; Halekas, J. S.; Connerney, J. E. P.; Curry, S.; Harada, Y.; Luhmann, J. G.; Jakosky, B. M.

2015-12-01

The Mars-solar wind interaction accelerates and transports planetary ions away from Mars through a number of processes, including pick-up by the electromagnetic fields. The Mars Atmospheric and Volatile EvolutioN (MAVEN) spacecraft has frequently detected strong escaping planetary ion fluxes in both tailward and upstream solar wind motional electric field directions since the beginning of its science phase in November 2014. Our statistical study using three-month MAVEN data from November 2014 through February 2015 illustrates a substantial plume-like escaping planetary ion population organized by the upstream electric field with strong fluxes widely distributed in the northern hemisphere of the Mars-Sun-Electric-field (MSE) coordinate system, which is generally consistent with model predictions. The plume constitutes an important planetary ion escape channel from the Martian atmosphere in addition to the tailward escape. The >25eV O+ escape rate through the plume is estimated to be ~35% of the tailward escape and ~25% of the total escape. We will compare the dynamics of the plume and tailward escaping ions based on their velocity-space distributions with respect to the electromagnetic fields. We will also discuss the variations of the plume characteristics between different ion species (O+, O2+, and CO2+) and from the effect of different solar wind and interplanetary magnetic field (IMF) conditions.

Unleashing Lessons: Sharing Stories About the Fine Art of Systems Engineering

NASA Technical Reports Server (NTRS)

Singer, Christopher E.

2010-01-01

NASA leaders have a responsibility to share their unique oral histories with junior-level employees on whom NASA's future depends. This presentation will give a few examples of how the imaginative, flexible art of systems engineering is as necessary to mission success as is the rigorous, disciplined side of engineering. Engineering space systems involves many disciplines propulsion, loads, dynamics, and so forth that are based on the foundations of scientific principles and methodology and the application of the laws of physics. The term rocket scientist is an apt term, considering that the underlying chemical properties of propellants and the subatomic properties of materials must be understood to harness the powerful energy necessary to escape Earth's gravity in machines that can withstand the stresses and forces to which they are subjected, not to mention the harsh space environments in which they must work. This is a simplistic, yet illustrative, explanation of the scientific side of the engineer s challenge. Bringing together these individual parts into a solid system goes beyond the science of engineering to employ the art of systems engineering. Systems engineers are known for their ability to integrate various solutions to meet or exceed challenging requirements. As the old adage goes, measure twice and cut once. The act of measuring is balancing rigid, inflexible requirements with creative compromises to attain the optimum solution to the challenge of space flight. Then, we cut out those answers that are too risky, expensive, dangerous, and so forth. The process of sharing stories about the little-discussed art of engineering, also known as the art of compromise, will equip the workforce to subjectively judge the best right answer from among the many presented, while objectively integrating the various piece parts into a unified whole.

78 FR 70391 - Shipping Coordinating Committee; Notice of Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-25

... II-1 subdivision and damage stability regulations --Development of guidelines on safe return to port... SOLAS regulation II-1/13.6 on means of escape from ro-ro cargo spaces --Classification of offshore...

Light Activated Escape Circuits: A Behavior and Neurophysiology Lab Module using Drosophila Optogenetics

PubMed Central

Titlow, Josh S.; Johnson, Bruce R.; Pulver, Stefan R.

2015-01-01

The neural networks that control escape from predators often show very clear relationships between defined sensory inputs and stereotyped motor outputs. This feature provides unique opportunities for researchers, but it also provides novel opportunities for neuroscience educators. Here we introduce new teaching modules using adult Drosophila that have been engineered to express csChrimson, a red-light sensitive channelrhodopsin, in specific sets of neurons and muscles mediating visually guided escape behaviors. This lab module consists of both behavior and electrophysiology experiments that explore the neural basis of flight escape. Three preparations are described that demonstrate photo-activation of the giant fiber circuit and how to quantify these behaviors. One of the preparations is then used to acquire intracellular electrophysiology recordings from different flight muscles. The diversity of action potential waveforms and firing frequencies observed in the flight muscles make this a rich preparation to study the ionic basic of cellular excitability. By activating different cells within the giant fiber pathway we also demonstrate principles of synaptic transmission and neural circuits. Beyond conveying core neurobiological concepts it is also expected that using these cutting edge techniques will enhance student motivation and attitudes towards biological research. Data collected from students and educators who have been involved in development of the module are presented to support this notion. PMID:26240526

Advanced Crew Escape Suit.

PubMed

1995-09-01

Design of the S1032 Launch Entry Suit (LES) began following the Challenger loss and NASA's decision to incorporate a Shuttle crew escape system. The LES (see Figure 1) has successfully supported Shuttle missions since NASA's Return to Flight with STS-26 in September 1988. In 1990, engineers began developing the S1035 Advanced Crew Escape Suit (ACES) to serve as a replacement for the LES. The ACES was designed to be a simplified, lightweight, low-bulk pressure suit which aided self donning/doffing, provided improved comfort, and enhanced overall performance to reduce crew member stress and fatigue. Favorable crew member evaluations of a prototype led to full-scale development and qualification of the S1035 ACES between 1990 and 1992. Production of the S1035 ACES began in February 1993, with the first unit delivered to NASA in May 1994. The S1035 ACES first flew aboard STS-68 in August 1994 and will become the primary crew escape suit when the S1032 LES ends its service life in late 1995. The primary goal of the S1035 development program was to provide improved performance over that of the S1032 to minimize the stress and fatigue typically experienced by crew members. To achieve this, five fundamental design objectives were established, resulting in various material/configuration changes.

Reporter Assay for Endo/Lysosomal Escape of Toxin-Based Therapeutics

PubMed Central

Gilabert-Oriol, Roger; Thakur, Mayank; von Mallinckrodt, Benedicta; Bhargava, Cheenu; Wiesner, Burkhard; Eichhorst, Jenny; Melzig, Matthias F.; Fuchs, Hendrik; Weng, Alexander

2014-01-01

Protein-based therapeutics with cytosolic targets are capable of exhibiting their therapeutic effect once they have escaped from the endosomes or lysosomes. In this study, the reporters—horseradish peroxidase (HRP), Alexa Fluor 488 (Alexa) and ricin A-chain (RTA)—were investigated for their capacity to monitor the endo/lysosomal escape of the ribosome-inactivating protein, saporin. The conjugates—saporin-HRP, Alexasaporin and saporin-KQ-RTA—were constructed, and the endo/lysosomal escape of these conjugates alone (lack of endo/lysosomal release) or in combination with certain structurally-specific triterpenoidal saponins (efficient endo/lysosomal escape) was characterized. HRP failed in reporting the endo/lysosomal escape of saporin. Contrastingly, Alexa Fluor 488 successfully allowed the report of the process at a toxin concentration of 1000 nM. In addition, single endo/lysosome analysis facilitated the determination of the amount of Alexasaporin released from each vesicle. RTA was also successful in reporting the endo/lysosomal escape of the enzymatically inactive mutant, saporin-KQ, but in this case, the sensitivity of the method reached a toxin concentration of 10 nM. In conclusion, the simultaneous usage of Alexa Fluor 488 and RTA as reporters may provide the possibility of monitoring the endo/lysosomal escape of protein-based therapeutics in the concentration range of 10–1000 nM. PMID:24859158

Never ever? Characteristics, outcomes and motivations of patients who abscond or escape: A 5-year review of escapes and absconds from two medium and low secure forensic units.

PubMed

Mezey, Gillian; Durkin, Catherine; Dodge, Liam; White, Sarah

2015-12-01

Absconds and escapes by psychiatric patients from secure forensic psychiatric settings create public anxiety and are poorly understood. To describe secure hospital patients who escape from within the secure perimeter or abscond, and test for differences between these groups. Escapes and absconds between 2008 and 2012 from the medium and low secure forensic psychiatric inpatient units of two London National Health Service Trusts were identified through the Trusts' databases. Demographic, offending, mental health and incident data were extracted from records for each. Seventy-seven incidents, involving 54 patients, were identified over the five years. These were 13 escapes involving 12 patients, representing a rate of 0.04 per 1000 bed days, and 64 absconds involving 42 patients, a rate of 0.26 per 1000 bed days; 15 (28%) patients were absent without leave more than once. Over half of the patients came back voluntarily within 24 hours of leaving. Over 50% of them had drunk alcohol or taken drugs while away from the unit. Escapees were more likely to be transferred prisoners and to have planned their escape, less likely to return to the unit voluntarily and away longer than patients who absconded. Offending was rare during unauthorised leave--just three offences among the 77 incidents; self-harm was more likely. Motives for absconding included: wanting freedom or drink or drugs, family worries and/or dissatisfaction with aspects of treatment. Escapes or absconding from secure healthcare units have different characteristics, but may best be prevented by convergent strategies. Relational security is likely to be as important for foiling plans for the former as it is for reducing boredom, building strong family support and managing substance misuse in the latter. Copyright © 2015 John Wiley & Sons, Ltd.

EXTENDED FERTILITY AND COMPATIBILITY OF PROGENY WITHINT HE AGROSTIS HYBRIDIZING COMPLEX: IMPLICATIONS FOR TRANSGENE ESCAPE AND PERSISTENCE WITHIN WILD POPULATION

EPA Science Inventory

Agrostis stolonifera L. (creeping bentgrass) is a turf grass that is of interest for introduction of herbicide and disease resistance, and stress tolerance traits by genetic engineering. A. stolonifera is a member of a hybridizing complex that includes at least eleven Agrostis sp...

Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae.

PubMed

Agmon, Neta; Tang, Zuojian; Yang, Kun; Sutter, Ben; Ikushima, Shigehito; Cai, Yizhi; Caravelli, Katrina; Martin, James A; Sun, Xiaoji; Choi, Woo Jin; Zhang, Allen; Stracquadanio, Giovanni; Hao, Haiping; Tu, Benjamin P; Fenyo, David; Bader, Joel S; Boeke, Jef D

2017-02-21

As the use of synthetic biology both in industry and in academia grows, there is an increasing need to ensure biocontainment. There is growing interest in engineering bacterial- and yeast-based safeguard (SG) strains. First-generation SGs were based on metabolic auxotrophy; however, the risk of cross-feeding and the cost of growth-controlling nutrients led researchers to look for other avenues. Recent strategies include bacteria engineered to be dependent on nonnatural amino acids and yeast SG strains that have both transcriptional- and recombinational-based biocontainment. We describe improving yeast Saccharomyces cerevisiae -based transcriptional SG strains, which have near-WT fitness, the lowest possible escape rate, and nanomolar ligands controlling growth. We screened a library of essential genes, as well as the best-performing promoter and terminators, yielding the best SG strains in yeast. The best constructs were fine-tuned, resulting in two tightly controlled inducible systems. In addition, for potential use in the prevention of industrial espionage, we screened an array of possible "decoy molecules" that can be used to mask any proprietary supplement to the SG strain, with minimal effect on strain fitness.

Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes

PubMed Central

Cai, Yizhi; Agmon, Neta; Choi, Woo Jin; Ubide, Alba; Stracquadanio, Giovanni; Caravelli, Katrina; Hao, Haiping; Bader, Joel S.; Boeke, Jef D.

2015-01-01

Biocontainment may be required in a wide variety of situations such as work with pathogens, field release applications of engineered organisms, and protection of intellectual properties. Here, we describe the control of growth of the brewer’s yeast, Saccharomyces cerevisiae, using both transcriptional and recombinational “safeguard” control of essential gene function. Practical biocontainment strategies dependent on the presence of small molecules require them to be active at very low concentrations, rendering them inexpensive and difficult to detect. Histone genes were controlled by an inducible promoter and controlled by 30 nM estradiol. The stability of the engineered genes was separately regulated by the expression of a site-specific recombinase. The combined frequency of generating viable derivatives when both systems were active was below detection (<10−10), consistent with their orthogonal nature and the individual escape frequencies of <10−6. Evaluation of escaper mutants suggests strategies for reducing their emergence. Transcript profiling and growth test suggest high fitness of safeguarded strains, an important characteristic for wide acceptance. PMID:25624482

13C and 15N fractionation of CH4/N2 mixtures during photochemical aerosol formation: Relevance to Titan

NASA Astrophysics Data System (ADS)

Sebree, Joshua A.; Stern, Jennifer C.; Mandt, Kathleen E.; Domagal-Goldman, Shawn D.; Trainer, Melissa G.

2016-05-01

The ratios of the stable isotopes that comprise each chemical species in Titan's atmosphere provide critical information towards understanding the processes taking place within its modern and ancient atmosphere. Several stable isotope pairs, including 12C/13C and 14N/15N, have been measured in situ or probed spectroscopically by Cassini-borne instruments, space telescopes, or through ground-based observations. Current attempts to model the observed isotope ratios incorporate fractionation resulting from atmospheric diffusion, hydrodynamic escape, and primary photochemical processes. However, the effect of a potentially critical pathway for isotopic fractionation - organic aerosol formation and subsequent deposition onto the surface of Titan - has not been considered due to insufficient data regarding fractionation during aerosol formation. To better understand the nature of this process, we have conducted a laboratory study to measure the isotopic fractionation associated with the formation of Titan aerosol analogs, commonly referred to as 'tholins', via far-UV irradiation of several methane (CH4) and dinitrogen (N2) mixtures. Analysis of the δ13C and δ15N isotopic signatures of the photochemical aerosol products using an isotope ratio mass spectrometer (IRMS) show that fractionation direction and magnitude are dependent on the initial bulk composition of the gas mixture. In general, the aerosols showed enrichment in 13C and 14N, and the observed fractionation trends can provide insight into the chemical mechanisms controlling photochemical aerosol formation.

Process and apparatus for afterburning of combustible pollutants from an internal combustion engine

DOE Office of Scientific and Technical Information (OSTI.GOV)

Laurent, P.A.

1978-07-04

In a process for the afterburning of the combustible pollutants from an internal combustion engine, in order to automatically reduce the secondary induction rate when power increases without using a controlling valve actuatd by the carburetor venturi depression, there is provided a volumetric efficiency of the secondary air pump linked to and activated by the engine and a volumetric efficiency which decreases when the ratio between its back pressure and suction pressure increases, this reduction being achieved through the proper selection of the pump volumetric compression ratio r: between 0.6 c and 1.3 c when a steeply decreasing trend ismore » required, and above 1.3 c if a slower and slower decreasing trend is required. To perform this process an afterburner apparatus has a nitrogen oxide reducing catalyst placed inside the afterburner reactor on the gas stream immediately at the outlet of a torus, in which the gases are homogenized and their reaction with preinjection air is terminated.« less

Synchronous activity lowers the energetic cost of nest escape for sea turtle hatchlings.

PubMed

Rusli, Mohd Uzair; Booth, David T; Joseph, Juanita

2016-05-15

A potential advantage of group movement in animals is increased locomotion efficiency. This implies a reduced energetic cost for individuals that occur in larger groups such as herds, flocks and schools. When chelonian hatchlings hatch in the underground nest with finite energy for their post-hatching dispersal phase, they face the challenge of minimizing energetic expenditure while escaping the nest. The term 'social facilitation' has been used to describe the combined digging effort of sea turtle hatchlings during nest escape. Given that in a normal clutch, a substantial part of the energy reserve within the residual yolk is used by hatchlings in the digging out process, a decreased cohort size may reduce the energy reserve available to cross the beach and sustain the initial swimming frenzy. This hypothesis was experimentally tested by varying cohort size in hatchling green turtles (Chelonia mydas) and measuring energy expenditure during the nest escape process using open-flow respirometry. The energetic cost of escaping through 40 cm of sand was calculated to vary between 4.4 and 28.3 kJ per individual, the cost decreasing as the number of individuals in the cohort increased. This represents 11-68% of the energy contained in a hatchling's residual yolk at hatching. The reduced energetic cost associated with large cohorts resulted from both a lower metabolic rate per individual and a shortened nest escape time. We conclude that synchronous digging activity of many hatchlings during nest escape evolved not only to facilitate rapid nest emergence but also to reduce the energetic cost to individuals. © 2016. Published by The Company of Biologists Ltd.

Tectonic escape in the evolution of the continental crust

NASA Technical Reports Server (NTRS)

Burke, K.; Sengor, C.

1986-01-01

The continental crust originated by processes similar to those operating today and continents consist of material most of which originated long ago in arc-systems that have later been modified, especially at Andean margins and in continental collisions where crustal thickening is common. Collision-related strike-slip motion is a general process in continental evolution. Because buoyant continental (or arc) material generally moves during collision toward a nearby oceanic margin where less buoyant lithosphere crops out, the process of major strike-slip dominated motion toward a 'free-face' is called 'tectonic escape'. Tectonic escape is and has been an element in continental evolution throughout recorded earth-history. It promotes: (1) rifting and the formation of rift-basins with thinning of thickened crust; (2) pervasive strike-slip faulting late in orogenic history which breaks up mountain belts across strike and may juxtapose unrelated sectors in cross-section; (3) localized compressional mountains and related foreland-trough basins.

Modulation of terrestrial ion escape flux composition /by low-altitude acceleration and charge exchange chemistry/

NASA Technical Reports Server (NTRS)

Moore, T. E.

1980-01-01

Motivated by recent observations of highly variable hot plasma composition in the magnetosphere, control of the ionospheric escape flux composition by low-altitude particle dynamics and ion chemistry has been investigated for an e(-), H(+), O(+) ionosphere. It is found that the fraction of the steady state escape flux which is O(+) can be controlled very sensitively by the occurrence of parallel or transverse ion acceleration at altitudes below the altitude where the neutral oxygen density falls rapidly below the neutral hydrogen density and the ionospheric source of O(+) tends to be rapidly converted by charge exchange to H(+). The acceleration is required both to overcome the gravitational confinement of O(+) and to violate charge exchange equilibrium so that the neutral hydrogen atmosphere appears 'optically' thin to escaping O(+). Constraints are placed on the acceleration processes, and it is shown that O(+) escape is facilitated by observed ionospheric responses to magnetic activity.

Biogenic methane, hydrogen escape, and the irreversible oxidation of early Earth.

PubMed

Catling, D C; Zahnle, K J; McKay, C

2001-08-03

The low O2 content of the Archean atmosphere implies that methane should have been present at levels approximately 10(2) to 10(3) parts per million volume (ppmv) (compared with 1.7 ppmv today) given a plausible biogenic source. CH4 is favored as the greenhouse gas that countered the lower luminosity of the early Sun. But abundant CH4 implies that hydrogen escapes to space (upward arrow space) orders of magnitude faster than today. Such reductant loss oxidizes the Earth. Photosynthesis splits water into O2 and H, and methanogenesis transfers the H into CH4. Hydrogen escape after CH4 photolysis, therefore, causes a net gain of oxygen [CO2 + 2H2O --> CH4 + 2O2 --> CO2 + O2 + 4H(upward arrow space)]. Expected irreversible oxidation (approximately 10(12) to 10(13) moles oxygen per year) may help explain how Earth's surface environment became irreversibly oxidized.

Studies of Fission Fragment Rocket Engine Propelled Spacecraft

NASA Technical Reports Server (NTRS)

Werka, Robert O.; Clark, Rodney; Sheldon, Rob; Percy, Thomas K.

2014-01-01

The NASA Office of Chief Technologist has funded from FY11 through FY14 successive studies of the physics, design, and spacecraft integration of a Fission Fragment Rocket Engine (FFRE) that directly converts the momentum of fission fragments continuously into spacecraft momentum at a theoretical specific impulse above one million seconds. While others have promised future propulsion advances if only you have the patience, the FFRE requires no waiting, no advances in physics and no advances in manufacturing processes. Such an engine unequivocally can create a new era of space exploration that can change spacecraft operation. The NIAC (NASA Institute for Advanced Concepts) Program Phase 1 study of FY11 first investigated how the revolutionary FFRE technology could be integrated into an advanced spacecraft. The FFRE combines existent technologies of low density fissioning dust trapped electrostatically and high field strength superconducting magnets for beam management. By organizing the nuclear core material to permit sufficient mean free path for escape of the fission fragments and by collimating the beam, this study showed the FFRE could convert nuclear power to thrust directly and efficiently at a delivered specific impulse of 527,000 seconds. The FY13 study showed that, without increasing the reactor power, adding a neutral gas to the fission fragment beam significantly increased the FFRE thrust through in a manner analogous to a jet engine afterburner. This frictional interaction of gas and beam resulted in an engine that continuously produced 1000 pound force of thrust at a delivered impulse of 32,000 seconds, thereby reducing the currently studied DRM 5 round trip mission to Mars from 3 years to 260 days. By decreasing the gas addition, this same engine can be tailored for much lower thrust at much higher impulse to match missions to more distant destinations. These studies created host spacecraft concepts configured for manned round trip journeys. While the vehicles are very large, they are primarily made up of a habitat payload on one end, the engine on the opposite end and a connecting spine containing radiator acreage needed to reject the heat of this powerful, but inefficient engine. These studies concluded that the engine and spacecraft are within today's technology, could be built, tested, launched on several SLS launchers, integrated, checked out, maintained at an in-space LEO base, and operated for decades just as Caribbean cruise ships operate today. The nuclear issues were found to be far less daunting that [than for] current nuclear engines. The FFRE produces very small amounts of radioactive efflux compared to their impulse, easily contained in an evacuated "bore-hole" test site. The engine poses no launch risk since it is simply a structure containing no fissionable material. The nuclear fuel is carried to orbit in containers highly crash-proofed for launch accidents from which it, in a liquid medium, is injected into the FFRE. The radioactive exhaust, with a velocity above 300 kilometers per second rapidly leaves the solar system.

International Conference on Defects-Recognition, Imaging and Physics in Semiconductors (13th), held at Wheeling, West Virginia, on 13-17 September 2009

DTIC Science & Technology

2010-06-01

Jansson, Y. Leterrier, and J.A.E. Manson, Engi- neering Fracture Mechanics . 37 (2006), pp. 2614-2626. 43. N.E. Jansson et al., Thin Solid Films, 515...ceremony in Octo- ber. Apelian is the Howmet Professor of Mechanical Engineering and direc- tor of the Metal Processing Institute at Worcester... mechanical engineering to mate- rials as an undergraduate student at the Indian Institute of Technology Kanpur. "I realized that major changes in

Where Are the People? The Human Viewpoint Approach for Architecting and Acquisition

DTIC Science & Technology

2014-10-01

however, systems engineers currently do not have sufficient tools to quantitatively integrate human considerations into systems development ( Hardman ...Engineering, 13(1), 72–79. Hardman , N., & Colombi, J. (2012). An empirical methodology for human integration in the SE technical processes. Journal of Systems

IDC Re-Engineering Phase 2 Glossary Version 1.3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Young, Christopher J.; Harris, James M.

2017-01-01

This document contains the glossary of terms used for the IDC Re-Engineering Phase 2 project. This version was created for Iteration E3. The IDC applies automatic processing methods in order to produce, archive, and distribute standard IDC products on behalf of all States Parties.

Dications and thermal ions in planetary atmospheric escape

NASA Astrophysics Data System (ADS)

Lilensten, J.; Simon Wedlund, C.; Barthélémy, M.; Thissen, R.; Ehrenreich, D.; Gronoff, G.; Witasse, O.

2013-01-01

In the recent years, the presence of dications in the atmospheres of Mars, Venus, Earth and Titan has been modeled and assessed. These studies also suggested that these ions could participate to the escape of the planetary atmospheres because a large fraction of them is unstable and highly energetic. When they dissociate, their internal energy is transformed into kinetic energy which may be larger than the escape energy. The goal of this study is to assess the impact of the doubly-charged ions in the escape of CO2-dominated planetary atmospheres and to compare it to the escape of thermal photo-ions. We solve a Boltzmann transport equation at daytime taking into account the dissociative states of CO2++ for a simplified single constituent atmosphere of a case-study planet. We compute the escape of fast ions using a Beer-Lambert approach. We study three test-cases. On a Mars-analog planet in today's conditions, we retrieve the measured electron escape flux. When comparing the two mechanisms (i.e. excluding solar wind effects, sputtering, etc.), the escape due to the fast ions issuing from the dissociation of dications may account for up to 6% of the total and the escape of thermal ions for the remaining. We show that these two mechanisms cannot explain the escape of the atmosphere since the magnetic field vanished and even contribute only marginally to this loss. We show that with these two mechanisms, the atmosphere of a Mars analog planet would empty in another giga years and a half. At Venus orbit, the contribution of the dications in the escape rate is negligible. When simulating the hot Jupiter HD 209458 b, the two processes cannot explain the measured escape flux of C+. This study shows that the dications may constitute a source of the escape of planetary atmospheres which had not been taken into account until now. This source, although marginal, is not negligible. The influence of the photoionization is of course large, but cannot explain alone the loss of Mars' atmosphere nor the atmospheric escape of HD 209458 b.

IEEE Computer Society/Software Engineering Institute Watts S. Humphrey Software Process Achievement Award 2016: Raytheon Integrated Defense Systems Design for Six Sigma Team

DTIC Science & Technology

2017-04-01

notice for non -US Government use and distribution. External use: This material may be reproduced in its entirety, without modification, and freely...Combinatorial Design Methods 4 2.1 Identification of Significant Improvement Opportunity 4 2.2 Methodology Development 4 2.3 Piloting...11 3 Process Performance Modeling and Analysis 13 3.1 Identification of Significant Improvement Opportunity 13 3.2 Methodology Development 13 3.3

An anticipative escape system for vehicles in water crashes

NASA Astrophysics Data System (ADS)

Shen, Chuanliang; Wang, Jiawei; Yin, Qi; Zhu, Yantao; Yang, Jiawei; Liao, Mengdi; Yang, Liming

2017-07-01

In this article, it designs an escape system for vehicles in water crashes. The structure mainly contains sensors, control organs and actuating mechanism for both doors and windows. Sensors judge whether the vehicle falls into water or is in the falling process. The actuating mechanism accepts the signal delivered by the control organs, then open the electronic central lock on doors and meanwhile lower the window. The water escape system is able to anticipate drowning situations for vehicles and controls both doors and windows in such an emergency. Under the premise of doors staying in an undamaged state, it is for sure that people in the vehicle can open the door while drowning in the water and safely escape.

Fluctuating bottleneck model studies on kinetics of DNA escape from α-hemolysin nanopores

NASA Astrophysics Data System (ADS)

Bian, Yukun; Wang, Zilin; Chen, Anpu; Zhao, Nanrong

2015-11-01

We have proposed a fluctuation bottleneck (FB) model to investigate the non-exponential kinetics of DNA escape from nanometer-scale pores. The basic idea is that the escape rate is proportional to the fluctuating cross-sectional area of DNA escape channel, the radius r of which undergoes a subdiffusion dynamics subjected to fractional Gaussian noise with power-law memory kernel. Such a FB model facilitates us to obtain the analytical result of the averaged survival probability as a function of time, which can be directly compared to experimental results. Particularly, we have applied our theory to address the escape kinetics of DNA through α-hemolysin nanopores. We find that our theoretical framework can reproduce the experimental results very well in the whole time range with quite reasonable estimation for the intrinsic parameters of the kinetics processes. We believe that FB model has caught some key features regarding the long time kinetics of DNA escape through a nanopore and it might provide a sound starting point to study much wider problems involving anomalous dynamics in confined fluctuating channels.

Distinct Escape Pathway by Hepatitis C Virus Genotype 1a from a Dominant CD8+ T Cell Response by Selection of Altered Epitope Processing.

PubMed

Walker, Andreas; Skibbe, Kathrin; Steinmann, Eike; Pfaender, Stephanie; Kuntzen, Thomas; Megger, Dominik A; Groten, Svenja; Sitek, Barbara; Lauer, Georg M; Kim, Arthur Y; Pietschmann, Thomas; Allen, Todd M; Timm, Joerg

2016-01-01

Antiviral CD8(+) T cells are a key component of the adaptive immune response against HCV, but their impact on viral control is influenced by preexisting viral variants in important target epitopes and the development of viral escape mutations. Immunodominant epitopes highly conserved across genotypes therefore are attractive for T cell based prophylactic vaccines. Here, we characterized the CD8(+) T cell response against the highly conserved HLA-B*51-restricted epitope IPFYGKAI1373-1380 located in the helicase domain of NS3 in people who inject drugs (PWID) exposed predominantly to HCV genotypes 1a and 3a. Despite this epitope being conserved in both genotypes, the corresponding CD8(+) T cell response was detected only in PWID infected with genotype 3a and HCV-RNA negative PWID, but not in PWID infected with genotype 1a. In genotype 3a, the detection of strong CD8(+) T cell responses was associated with epitope variants in the autologous virus consistent with immune escape. Analysis of viral sequences from multiple cohorts confirmed HLA-B*51-associated escape mutations inside the epitope in genotype 3a, but not in genotype 1a. Here, a distinct substitution in the N-terminal flanking region located 5 residues upstream of the epitope (S1368P; P = 0.00002) was selected in HLA-B*51-positive individuals. Functional assays revealed that the S1368P substitution impaired recognition of target cells presenting the endogenously processed epitope. The results highlight that, despite an epitope being highly conserved between two genotypes, there are major differences in the selected viral escape pathways and the corresponding T cell responses. HCV is able to evolutionary adapt to CD8(+) T cell immune pressure in multiple ways. Beyond selection of mutations inside targeted epitopes, this study demonstrates that HCV inhibits epitope processing by modification of the epitope flanking region under T cell immune pressure. Selection of a substitution five amino acids upstream of the epitope underlines that efficient antigen presentation strongly depends on its larger sequence context and that blocking of the multistep process of antigen processing by mutation is exploited also by HCV. The pathways to mutational escape of HCV are to some extent predictable but are distinct in different genotypes. Importantly, the selected escape pathway of HCV may have consequences for the destiny of antigen-specific CD8(+) T cells. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Analyzing Systems Integration Best Practices and Assessment in DoD Space Systems Acquisition

DTIC Science & Technology

2009-12-01

satellite  Insufficient stress relief and insulation caused abrasion of wiring harness. C Product–Product: stress relief and insulation – wiring...delaminated during firing . This problem escaped qualification since slow heating rates (0.1–deg F/sec) used in the lab test provided time for the gas...to escape. Faster rates would have revealed the issue. E Product–Process: material – replace, firing ; rate – test B Process–Process: replace

NASA's first Orion full-scale abort flight test crew module was placed in NASA Dryden's Abort Flight Test integration area for equipment installation.

NASA Image and Video Library

2008-04-01

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

NASA Dryden Flight Research Center personnel accompany NASA's first Orion full-scale abort flight test crew module as it heads to its new home.

NASA Image and Video Library

2008-04-01

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

A NASA painter applies the first primer coat to NASA's Orion full-scale abort flight test crew module in the Edwards Air Force Base paint hangar.

NASA Image and Video Library

2008-03-29

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

Air Force loadmasters oversee unloading of the full-scale Orion abort test crew module mockup from a C-17 cargo aircraft at Edwards Air Force Base March 28.

NASA Image and Video Library

2008-03-28

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

Paint shop technicians carefully apply masking prior to painting the Orion full-scale abort flight test crew module in the Edwards Air Force Base paint hangar.

NASA Image and Video Library

2008-03-29

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

NASA paint shop technicians prepare the Orion full-scale flight test crew module for painting in the Edwards Air Force Base paint hangar.

NASA Image and Video Library

2008-03-29

A full-scale flight-test mockup of the Constellation program's Orion crew vehicle arrived at NASA's Dryden Flight Research Center in late March 2008 to undergo preparations for the first short-range flight test of the spacecraft's astronaut escape system later that year. Engineers and technicians at NASA's Langley Research Center fabricated the structure, which precisely represents the size, outer shape and mass characteristics of the Orion space capsule. The Orion crew module mockup was ferried to NASA Dryden on an Air Force C-17. After painting in the Edwards Air Force Base paint hangar, the conical capsule was taken to Dryden for installation of flight computers, instrumentation and other electronics prior to being sent to the U.S. Army's White Sands Missile Range in New Mexico for integration with the escape system and the first abort flight test in late 2008. The tests were designed to ensure a safe, reliable method of escape for astronauts in case of an emergency.

Saturn Apollo Program

NASA Image and Video Library

1967-03-01

The Saturn V configuration is shown in inches and meters as illustrated by the Boeing Company. The Saturn V vehicle consisted of three stages: the S-IC (first) stage powered by five F-1 engines, the S-II (second) stage powered by five J-2 engines, the S-IVB (third) stage powered by one J-2 engine. A top for the first three stages was designed to contain the instrument unit, the guidance system, the Apollo spacecraft, and the escape system. The Apollo spacecraft consisted of the lunar module, the service module, and the command module. The Saturn V was designed perform lunar and planetary missions and it was capable of placing 280,000 pounds into Earth orbit.

Measurement of the Neutron Flux of the Reaction C$sup 12$(d,n)N$sup 13$ Based on the Activity of N$sup 13$. Report No. 28; MEDICION DEL FLUJO NEUTRONICO DE LA REACCION C$sup 12$(d,n)N$sup 13$ A BASE DE LA ACTIVIDAD DEL N$sup 13$. INFORME NO 28

DOE Office of Scientific and Technical Information (OSTI.GOV)

Keller, A.

1960-08-01

The principal problem in the measurement of neutron flux from the reaction C/sup 12/(d,n)N/sup 13/, based on the activity of N/sup 13/, is the possibility of nitrogen escape. A method for the measurement of possibie N/sup 13/ losses is described, and measurements with TiC targets irradiated with 600- kev deuterons are reported. The formulas for the calculation of the neutron flux are indicated. (tr-auth)

Escape rates over potential barriers: variational principles and the Hamilton-Jacobi equation

NASA Astrophysics Data System (ADS)

Cortés, Emilio; Espinosa, Francisco

We describe a rigorous formalism to study some extrema statistics problems, like maximum probability events or escape rate processes, by taking into account that the Hamilton-Jacobi equation completes, in a natural way, the required set of boundary conditions of the Euler-Lagrange equation, for this kind of variational problem. We apply this approach to a one-dimensional stochastic process, driven by colored noise, for a double-parabola potential, where we have one stable and one unstable steady states.

Systems Engineering Approach to Technology Integration for NASA's 2nd Generation Reusable Launch Vehicle

NASA Technical Reports Server (NTRS)

Thomas, Dale; Smith, Charles; Thomas, Leann; Kittredge, Sheryl

2002-01-01

The overall goal of the 2nd Generation RLV Program is to substantially reduce technical and business risks associated with developing a new class of reusable launch vehicles. NASA's specific goals are to improve the safety of a 2nd-generation system by 2 orders of magnitude - equivalent to a crew risk of 1-in-10,000 missions - and decrease the cost tenfold, to approximately $1,000 per pound of payload launched. Architecture definition is being conducted in parallel with the maturating of key technologies specifically identified to improve safety and reliability, while reducing operational costs. An architecture broadly includes an Earth-to-orbit reusable launch vehicle, on-orbit transfer vehicles and upper stages, mission planning, ground and flight operations, and support infrastructure, both on the ground and in orbit. The systems engineering approach ensures that the technologies developed - such as lightweight structures, long-life rocket engines, reliable crew escape, and robust thermal protection systems - will synergistically integrate into the optimum vehicle. To best direct technology development decisions, analytical models are employed to accurately predict the benefits of each technology toward potential space transportation architectures as well as the risks associated with each technology. Rigorous systems analysis provides the foundation for assessing progress toward safety and cost goals. The systems engineering review process factors in comprehensive budget estimates, detailed project schedules, and business and performance plans, against the goals of safety, reliability, and cost, in addition to overall technical feasibility. This approach forms the basis for investment decisions in the 2nd Generation RLV Program's risk-reduction activities. Through this process, NASA will continually refine its specialized needs and identify where Defense and commercial requirements overlap those of civil missions.

Systems Engineering Approach to Technology Integration for NASA's 2nd Generation Reusable Launch Vehicle

NASA Technical Reports Server (NTRS)

Thomas, Dale; Smith, Charles; Thomas, Leann; Kittredge, Sheryl

2002-01-01

The overall goal of the 2nd Generation RLV Program is to substantially reduce technical and business risks associated with developing a new class of reusable launch vehicles. NASA's specific goals are to improve the safety of a 2nd generation system by 2 orders of magnitude - equivalent to a crew risk of 1-in-10,000 missions - and decrease the cost tenfold, to approximately $1,000 per pound of payload launched. Architecture definition is being conducted in parallel with the maturating of key technologies specifically identified to improve safety and reliability, while reducing operational costs. An architecture broadly includes an Earth-to-orbit reusable launch vehicle, on-orbit transfer vehicles and upper stages, mission planning, ground and flight operations, and support infrastructure, both on the ground and in orbit. The systems engineering approach ensures that the technologies developed - such as lightweight structures, long-life rocket engines, reliable crew escape, and robust thermal protection systems - will synergistically integrate into the optimum vehicle. To best direct technology development decisions, analytical models are employed to accurately predict the benefits of each technology toward potential space transportation architectures as well as the risks associated with each technology. Rigorous systems analysis provides the foundation for assessing progress toward safety and cost goals. The systems engineering review process factors in comprehensive budget estimates, detailed project schedules, and business and performance plans, against the goals of safety, reliability, and cost, in addition to overall technical feasibility. This approach forms the basis for investment decisions in the 2nd Generation RLV Program's risk-reduction activities. Through this process, NASA will continually refine its specialized needs and identify where Defense and commercial requirements overlap those of civil missions.

Engineering nanoparticle-coated bacteria as oral DNA vaccines for cancer immunotherapy.

PubMed

Hu, Qinglian; Wu, Min; Fang, Chun; Cheng, Changyong; Zhao, Mengmeng; Fang, Weihuan; Chu, Paul K; Ping, Yuan; Tang, Guping

2015-04-08

Live attenuated bacteria are of increasing importance in biotechnology and medicine in the emerging field of cancer immunotherapy. Oral DNA vaccination mediated by live attenuated bacteria often suffers from low infection efficiency due to various biological barriers during the infection process. To this end, we herein report, for the first time, a new strategy to engineer cationic nanoparticle-coated bacterial vectors that can efficiently deliver oral DNA vaccine for efficacious cancer immunotherapy. By coating live attenuated bacteria with synthetic nanoparticles self-assembled from cationic polymers and plasmid DNA, the protective nanoparticle coating layer is able to facilitate bacteria to effectively escape phagosomes, significantly enhance the acid tolerance of bacteria in stomach and intestines, and greatly promote dissemination of bacteria into blood circulation after oral administration. Most importantly, oral delivery of DNA vaccines encoding autologous vascular endothelial growth factor receptor 2 (VEGFR2) by this hybrid vector showed remarkable T cell activation and cytokine production. Successful inhibition of tumor growth was also achieved by efficient oral delivery of VEGFR2 with nanoparticle-coated bacterial vectors due to angiogenesis suppression in the tumor vasculature and tumor necrosis. This proof-of-concept work demonstrates that coating live bacterial cells with synthetic nanoparticles represents a promising strategy to engineer efficient and versatile DNA vaccines for the era of immunotherapy.

30 CFR 784.16 - Reclamation plan: Siltation structures, impoundments, and refuse piles.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Resources Conservation Service's Web site athttp://www.info.usda.gov/scripts/lpsiis.dll/TR/TR_210_60.htm... State program approval process engineering design standards that ensure stability comparable to a 1.3 minimum static safety factor in lieu of engineering tests to establish compliance with the minimum static...

30 CFR 780.25 - Reclamation plan: Siltation structures, impoundments, and refuse piles.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Resources Conservation Service's Web site at http://www.info.usda.gov/scripts/lpsiis.dll/TR/TR_210_60.htm... authority may establish through the State program approval process, engineering design standards that ensure stability comparable to a 1.3 minimum static safety factor in lieu of engineering tests to establish...

Efficiently estimating salmon escapement uncertainty using systematically sampled data

USGS Publications Warehouse

Reynolds, Joel H.; Woody, Carol Ann; Gove, Nancy E.; Fair, Lowell F.

2007-01-01

Fish escapement is generally monitored using nonreplicated systematic sampling designs (e.g., via visual counts from towers or hydroacoustic counts). These sampling designs support a variety of methods for estimating the variance of the total escapement. Unfortunately, all the methods give biased results, with the magnitude of the bias being determined by the underlying process patterns. Fish escapement commonly exhibits positive autocorrelation and nonlinear patterns, such as diurnal and seasonal patterns. For these patterns, poor choice of variance estimator can needlessly increase the uncertainty managers have to deal with in sustaining fish populations. We illustrate the effect of sampling design and variance estimator choice on variance estimates of total escapement for anadromous salmonids from systematic samples of fish passage. Using simulated tower counts of sockeye salmon Oncorhynchus nerka escapement on the Kvichak River, Alaska, five variance estimators for nonreplicated systematic samples were compared to determine the least biased. Using the least biased variance estimator, four confidence interval estimators were compared for expected coverage and mean interval width. Finally, five systematic sampling designs were compared to determine the design giving the smallest average variance estimate for total annual escapement. For nonreplicated systematic samples of fish escapement, all variance estimators were positively biased. Compared to the other estimators, the least biased estimator reduced bias by, on average, from 12% to 98%. All confidence intervals gave effectively identical results. Replicated systematic sampling designs consistently provided the smallest average estimated variance among those compared.

Dependence of Photochemical Escape of Oxygen at Mars on Solar Radiation and Solar Wind Interaction

NASA Astrophysics Data System (ADS)

Cravens, T.; Rahmati, A.; Lillis, R. J.; Fox, J. L.; Bougher, S. W.; Jakosky, B. M.

2016-12-01

The evolution of the atmosphere of Mars and the loss of volatiles over the life of the solar system is a key topic in planetary science. An important loss process in the ionosphere is photochemical escape. In particular, dissociative recombination of O2+ ions (the major ion species) produces fast oxygen atoms, some of which can escape from the planet. Several theoretical models have been constructed over the years to study hot oxygen and its escape from Mars. These model have a number of uncertainties, particularly for the elastic cross sections of O collisions with target neutral species. Recently, the Mars Atmosphere and Volatile Evolution Mission (MAVEN) mission has been rapidly improving our understanding of the upper atmosphere and ionosphere of Mars and its interaction with the external environment (e.g., the solar wind). The purpose of the current paper is to take a simple analytical approach to the oxygen escape problem in order to: (1) study the role that solar flux and solar wind variations have on escape and (2) isolate the effects of uncertainties in oxygen cross sections on the derived oxygen escape rates. Not surprisingly, we find, in agreement with more elaborate numerical models, that the escape flux is directly proportional to the incident solar extreme ultraviolet irradiance and is inversely proportional to the backscatter elastic cross section. The role for atmospheric loss that ion transport plays in the topside ionosphere and how the solar wind interaction drives this will also be discussed.

On the verge of a respiratory-type panic attack: Selective activations of rostrolateral and caudoventrolateral periaqueductal gray matter following short-lasting escape to a low dose of potassium cyanide.

PubMed

Müller, Cláudia Janaina Torres; Quintino-Dos-Santos, Jeyce Willig; Schimitel, Fagna Giacomin; Tufik, Sérgio; Beijamini, Vanessa; Canteras, Newton Sabino; Schenberg, Luiz Carlos

2017-04-21

Intravenous injections of potassium cyanide (KCN) both elicit escape by its own and facilitate escape to electrical stimulation of the periaqueductal gray matter (PAG). Moreover, whereas the KCN-evoked escape is potentiated by CO 2 , it is suppressed by both lesions of PAG and clinically effective treatments with panicolytics. These and other data suggest that the PAG harbors a hypoxia-sensitive alarm system the activation of which could both precipitate panic and render the subject hypersensitive to CO 2 . Although prior c-Fos immunohistochemistry studies reported widespread activations of PAG following KCN injections, the employment of repeated injections of high doses of KCN (>60µg) in anesthetized rats compromised both the localization of KCN-responsive areas and their correlation with escape behavior. Accordingly, here we compared the brainstem activations of saline-injected controls (air/saline) with those produced by a single intravenous injection of 40-µg KCN (air/KCN), a 2-min exposure to 13% CO 2 (CO 2 /saline), or a combined stimulus (CO 2 /KCN). Behavioral effects of KCN microinjections into the PAG were assessed as well. Data showed that whereas the KCN microinjections were ineffective, KCN intravenous injections elicited escape in all tested rats. Moreover, whereas the CO 2 alone was ineffective, it potentiated the KCN-evoked escape. Compared to controls, the nucleus tractus solitarius was significantly activated in both CO 2 /saline and CO 2 /KCN groups. Additionally, whereas the laterodorsal tegmental nucleus was activated by all treatments, the rostrolateral and caudoventrolateral PAG were activated by air/KCN only. Data suggest that the latter structures are key components of a hypoxia-sensitive suffocation alarm which activation may trigger a panic attack. Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.

A convergent and essential interneuron pathway for Mauthner-cell-mediated escapes.

PubMed

Lacoste, Alix M B; Schoppik, David; Robson, Drew N; Haesemeyer, Martin; Portugues, Ruben; Li, Jennifer M; Randlett, Owen; Wee, Caroline L; Engert, Florian; Schier, Alexander F

2015-06-01

The Mauthner cell (M-cell) is a command-like neuron in teleost fish whose firing in response to aversive stimuli is correlated with short-latency escapes [1-3]. M-cells have been proposed as evolutionary ancestors of startle response neurons of the mammalian reticular formation [4], and studies of this circuit have uncovered important principles in neurobiology that generalize to more complex vertebrate models [3]. The main excitatory input was thought to originate from multisensory afferents synapsing directly onto the M-cell dendrites [3]. Here, we describe an additional, convergent pathway that is essential for the M-cell-mediated startle behavior in larval zebrafish. It is composed of excitatory interneurons called spiral fiber neurons, which project to the M-cell axon hillock. By in vivo calcium imaging, we found that spiral fiber neurons are active in response to aversive stimuli capable of eliciting escapes. Like M-cell ablations, bilateral ablations of spiral fiber neurons largely eliminate short-latency escapes. Unilateral spiral fiber neuron ablations shift the directionality of escapes and indicate that spiral fiber neurons excite the M-cell in a lateralized manner. Their optogenetic activation increases the probability of short-latency escapes, supporting the notion that spiral fiber neurons help activate M-cell-mediated startle behavior. These results reveal that spiral fiber neurons are essential for the function of the M-cell in response to sensory cues and suggest that convergent excitatory inputs that differ in their input location and timing ensure reliable activation of the M-cell, a feedforward excitatory motif that may extend to other neural circuits. Copyright © 2015 Elsevier Ltd. All rights reserved.

Life events and escape in conversion disorder.

PubMed

Nicholson, T R; Aybek, S; Craig, T; Harris, T; Wojcik, W; David, A S; Kanaan, R A

2016-09-01

Psychological models of conversion disorder (CD) traditionally assume that psychosocial stressors are identifiable around symptom onset. In the face of limited supportive evidence such models are being challenged. Forty-three motor CD patients, 28 depression patients and 28 healthy controls were assessed using the Life Events and Difficulties Schedule in the year before symptom onset. A novel 'escape' rating for events was developed to test the Freudian theory that physical symptoms of CD could provide escape from stressors, a form of 'secondary gain'. CD patients had significantly more severe life events and 'escape' events than controls. In the month before symptom onset at least one severe event was identified in 56% of CD patients - significantly more than 21% of depression patients [odds ratio (OR) 4.63, 95% confidence interval (CI) 1.56-13.70] and healthy controls (OR 5.81, 95% CI 1.86-18.2). In the same time period 53% of CD patients had at least one 'high escape' event - again significantly higher than 14% in depression patients (OR 6.90, 95% CI 2.05-23.6) and 0% in healthy controls. Previous sexual abuse was more commonly reported in CD than controls, and in one third of female patients was contextually relevant to life events at symptom onset. The majority (88%) of life events of potential aetiological relevance were not identified by routine clinical assessments. Nine per cent of CD patients had no identifiable severe life events. Evidence was found supporting the psychological model of CD, the Freudian notion of escape and the potential aetiological relevance of childhood traumas in some patients. Uncovering stressors of potential aetiological relevance requires thorough psychosocial evaluation.

Prototype Software Assurance Framework (SAF): Introduction and Overview

DTIC Science & Technology

2017-04-05

Introduction 1 1 Process Management (Category 1) 6 1.1 Process Definition (Area 1.1) 6 1.2 Infrastructure Standards (Area 1.2) 6 1.3 Resources (Area 1.3) 7...1.4 Training (Area 1.4) 8 2 Project Management (Category 2) 9 2.1 Project Plans (Area 2.1) 9 2.2 Project Infrastructure (Area 2.2) 10 2.3 Project...Monitoring (Area 2.3) 10 2.4 Project Risk Management (Area 2.4) 11 2.5 Supplier Management (Area 2.5) 11 3 Engineering (Category 3) 13 3.1 Product

Treatment of Industrial Process Effluents & Contaminated Groundwater Using the Biological Granular Activated Carbon-Fluidized Bed Reactor (GAC-FBR) Process. Volume I

DTIC Science & Technology

1996-09-30

Microbial transformation of nitroaromatics in surface soils and aquifer materials. Appl . Environ. Microbiol. 60:2170-2175. Crawford, R. L. 1995...Construction Engineering Research Laboratories, Corps of Engineers, P.O. Box 9005, Champaign, IL 61826-9005. This work was supported in part by SERDP. The...113 13. ABSTRACT (Maximum 200 Words) In 1992, Congress allocated funds for development of expertise in applied environmental bioremediation restoration

Developing the E-Scape Software System

ERIC Educational Resources Information Center

Derrick, Karim

2012-01-01

Most innovations have contextual pre-cursors that prompt new ways of thinking and in their turn help to give form to the new reality. This was the case with the e-scape software development process. The origins of the system existed in software components and ideas that we had developed through previous projects, but the ultimate direction we took…

Global trends on reef fishes' ecology of fear: Flight initiation distance for conservation.

PubMed

Nunes, José Anchieta C C; Costa, Yuri; Blumstein, Daniel T; Leduc, Antoine O H C; Dorea, Antônio C; Benevides, Larissa J; Sampaio, Cláudio L S; Barros, Francisco

2018-05-01

Escape behaviors have a great potential as an indicator of the efficacy of management. For instance, the degree of fear perceived by fishes targeted by fisheries is frequently higher in unprotected marine areas than in areas where some protection is provided. We systematically reviewed the literature on how fear, which we define as variation in escape behavior, was quantified in reef fishes. In the past 25 years, a total of 33 studies were identified, many of which were published within the last five years and nearly 40% of those (n = 13) focused on Indo-Pacific reefs, showing that there are still many geographical gaps. While eleven escape metrics were identified to evaluate fish escape, flight initiation distance (FID) was the most commonly employed (n = 23). FID was used to study different questions of applied and theoretical ecology, which involved 14 reef fish families. We also used a formal meta-analysis to investigate the effects of fishing by comparing FID inside and outside marine protected areas. Fishes outside MPAs had increased FID compared to those inside MPAs. The Labridae family had a significantly higher effect sizes than Acanthuridae and Epinephelidae, suggesting that fishes in this family may be indicators of effective MPAs using FID. We conclude that protocols aimed to quantify fear in fishes, which provide accurate assessments of fishing effects on fish escape behavior, will help gauge the compliance of marine protected areas. Copyright © 2018 Elsevier Ltd. All rights reserved.

76 FR 64287 - Airworthiness Directives; General Electric Company CF34-10E Series Turbofan Engines

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-18

... escape. Once removed, the CVD support assembly (consisting of self-locking nut, part number (P/N... location. The wear is caused by relative motion between the CVD support assembly (consisting of self... AD, do not return to service any CVD support assembly (consisting of self-locking nut, P/N 2226M57G03...

Progress and perspective of inorganic nanoparticles based siRNA delivery system

PubMed Central

Jiang, Ying; Huo, Shuaidong; Hardie, Joseph; Liang, Xing-Jie; Rotello, Vincent M.

2016-01-01

Introduction Small interfering RNA (siRNA) is an effective method for regulating the expression of proteins, even “undruggable” ones that are nearly impossible to target through traditional small molecule therapeutics. Delivery to the cell and then to the cytosol is the primary requirement for realization of therapeutic potential of siRNA. Areas covered We summarize recent advances in the design of inorganic nanoparticle with surface functionality and physicochemical properties engineered for siRNA delivery. Specifically, we discuss the main approaches developed so far to load siRNA into/onto NPs, and NP surface chemistry engineered for enhanced intracellular siRNA delivery, endosomal escape, and targeted delivery of siRNA to disease cells and tissues. Expert Opinion Several challenges remain in developing inorganic NPs for efficient and effective siRNA delivery. Getting the material to the chosen site is important, however the greatest hurdle may well be delivery into the cytosol, either through efficient endosomal escape or by direct cytosolic siRNA delivery. Effective delivery at the organismic and cellular level coupled with biocompatible vehicles with low immunogenic response will facilitate the clinical translation of RNAi for the treatment of genetic diseases. PMID:26735861

Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae

PubMed Central

Agmon, Neta; Tang, Zuojian; Yang, Kun; Sutter, Ben; Ikushima, Shigehito; Cai, Yizhi; Caravelli, Katrina; Martin, James A.; Sun, Xiaoji; Choi, Woo Jin; Zhang, Allen; Stracquadanio, Giovanni; Hao, Haiping; Tu, Benjamin P.; Fenyo, David; Bader, Joel S.

2017-01-01

As the use of synthetic biology both in industry and in academia grows, there is an increasing need to ensure biocontainment. There is growing interest in engineering bacterial- and yeast-based safeguard (SG) strains. First-generation SGs were based on metabolic auxotrophy; however, the risk of cross-feeding and the cost of growth-controlling nutrients led researchers to look for other avenues. Recent strategies include bacteria engineered to be dependent on nonnatural amino acids and yeast SG strains that have both transcriptional- and recombinational-based biocontainment. We describe improving yeast Saccharomyces cerevisiae-based transcriptional SG strains, which have near-WT fitness, the lowest possible escape rate, and nanomolar ligands controlling growth. We screened a library of essential genes, as well as the best-performing promoter and terminators, yielding the best SG strains in yeast. The best constructs were fine-tuned, resulting in two tightly controlled inducible systems. In addition, for potential use in the prevention of industrial espionage, we screened an array of possible “decoy molecules” that can be used to mask any proprietary supplement to the SG strain, with minimal effect on strain fitness. PMID:28174266

Life Cycle Systems Engineering Approach to NASA's 2nd Generation Reusable Launch Vehicle

NASA Technical Reports Server (NTRS)

Thomas, Dale; Smith, Charles; Safie, Fayssal; Kittredge, Sheryl

2002-01-01

The overall goal of the 2nd Generation RLV Program is to substantially reduce technical and business risks associated with developing a new class of reusable launch vehicles. NASA's specific goals are to improve the safety of a 2nd- generation system by 2 orders of magnitude - equivalent to a crew risk of 1 -in- 10,000 missions - and decrease the cost tenfold, to approximately $1,000 per pound of payload launched. Architecture definition is being conducted in parallel with the maturating of key technologies specifically identified to improve safety and reliability, while reducing operational costs. An architecture broadly includes an Earth-to-orbit reusable launch vehicle, on-orbit transfer vehicles and upper stages, mission planning, ground and flight operations, and support infrastructure, both on the ground and in orbit. The systems engineering approach ensures that the technologies developed - such as lightweight structures, long-life rocket engines, reliable crew escape, and robust thermal protection systems - will synergistically integrate into the optimum vehicle. Given a candidate architecture that possesses credible physical processes and realistic technology assumptions, the next set of analyses address the system's functionality across the spread of operational scenarios characterized by the design reference missions. The safety/reliability and cost/economics associated with operating the system will also be modeled and analyzed to answer the questions "How safe is it?" and "How much will it cost to acquire and operate?" The systems engineering review process factors in comprehensive budget estimates, detailed project schedules, and business and performance plans, against the goals of safety, reliability, and cost, in addition to overall technical feasibility. This approach forms the basis for investment decisions in the 2nd Generation RLV Program's risk-reduction activities. Through this process, NASA will continually refine its specialized needs and identify where Defense and commercial requirements overlap those of civil missions.

Reply to comment "On the hydrogen escape: Comment to variability of the hydrogen in the Martian upper atmosphere as simulated by a 3D atmosphere-exosphere coupling by J.-Y. Chaufray et al." by V. Krasnopolsky, Icarus, 281, 262

NASA Astrophysics Data System (ADS)

Chaufray, J.-Y.; Gonzalez-Galindo, F.; Forget, F.; Lopez-Valverde, M.; Leblanc, F.; Modolo, R.; Hess, S.

2018-02-01

Krasnopolsky (2017) makes a careful review of our recent results about the Martian hydrogen content of the Martian upper atmosphere (Chaufray et al., 2015). We comment here on his two major points. First, he suggests that the non-thermal escape of H2, and particularly collisions with hot oxygen, not taken into account in our general circulation model (GCM), should modify our reported H2 and H density profiles. This is an important issue; we acknowledge that future effective coupling of our GCM with comprehensive models of the Martian solar wind interaction, ideally after being validated with the latest plasma observations of H2+, would allow for better estimations of the relative importance of the H2 non-thermal and thermal escape processes. For the time being we need assumptions in the GCM, with proper and regular updates. According to a recent and detailed study of the anisotropic elastic and inelastic collision cross sections between O and H2 (Gacesa et al., 2012), the escape rates used by Krasnopolsky (2010) for this process might be overestimated. We therefore do not include non thermal escape of H2 in the model. And secondly, in response to Krasnopolsky's comment on the H escape variability with the solar cycle, we revised our calculations and found a small bug in the computation of the Jeans effusion velocity. Our revised computed H escape rates are included here. They have a small impact on our key conclusions: similar seasonal variations, a reduced variation with the solar cycle but still larger than Krasnopolsky (2017), and again a hydrogen scape systematically lower than the diffusion-limited flux. This bug does not affect the latest Mars Climate Database v5.2.

13. Photocopy of 1920 drawing titled: BUILDING 78, 3RD FLOOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Photocopy of 1920 drawing titled: BUILDING 78, 3RD FLOOR BALCONY AND FIRE ESCAPES, including plans for skylight and North Elevation. HABS photograph is an 8x10' contact print made from a high contrast negative of an enlargement made from microfiche. Original is in the collection of Department of Public Works, Puget Sound Naval Shipyard, Bremerton, WA. - Puget Sound Naval Shipyard, Administration Building, Farragut Avenue, Bremerton, Kitsap County, WA

Escape probability of the super-Penrose process

NASA Astrophysics Data System (ADS)

Ogasawara, Kota; Harada, Tomohiro; Miyamoto, Umpei; Igata, Takahisa

2017-06-01

We consider a head-on collision of two massive particles that move in the equatorial plane of an extremal Kerr black hole, which results in the production of two massless particles. Focusing on a typical case, where both of the colliding particles have zero angular momenta, we show that a massless particle produced in such a collision can escape to infinity with arbitrarily large energy in the near-horizon limit of the collision point. Furthermore, if we assume that the emission of the produced massless particles is isotropic in the center-of-mass frame but confined to the equatorial plane, the escape probability of the produced massless particle approaches 5 /12 , and almost all escaping massless particles have arbitrarily large energy at infinity and an impact parameter approaching 2 G M /c2, where M is the mass of the black hole.

Helicity charging and eruption of magnetic flux from the Sun

NASA Technical Reports Server (NTRS)

Rust, David M.; Kumar, A.

1994-01-01

The ejection of helical toroidal fields from the solar atmosphere and their detection in interplanetary space are described. The discovery that solar magnetic fields are twisted and that they are segregated by hemisphere according to their chirality has important implications for the escape process. The roles played by erupting prominences, coronal mass ejections (CME's) and active region (AR) loops in expressing the escape of magnetic flux and helicity are discussed. Sporadic flux escape associated with filament eruptions accounts for less than one-tenth the flux loss. Azimuthal flux loss by CME's could account for more, but the major contributor to flux escape may be AR loop expansion. It is shown how the transfer of magnetic helicity from the sun's interior into emerged loops ('helicity charging') could be the effective driver of solar eruptions and of flux loss from the sun.

Eradication of Large Solid Tumors by Gene Therapy with a T-Cell Receptor Targeting a Single Cancer-Specific Point Mutation.

PubMed

Leisegang, Matthias; Engels, Boris; Schreiber, Karin; Yew, Poh Yin; Kiyotani, Kazuma; Idel, Christian; Arina, Ainhoa; Duraiswamy, Jaikumar; Weichselbaum, Ralph R; Uckert, Wolfgang; Nakamura, Yusuke; Schreiber, Hans

2016-06-01

Cancers usually contain multiple unique tumor-specific antigens produced by single amino acid substitutions (AAS) and encoded by somatic nonsynonymous single nucleotide substitutions. We determined whether adoptively transferred T cells can reject large, well-established solid tumors when engineered to express a single type of T-cell receptor (TCR) that is specific for a single AAS. By exome and RNA sequencing of an UV-induced tumor, we identified an AAS in p68 (mp68), a co-activator of p53. This AAS seemed to be an ideal tumor-specific neoepitope because it is encoded by a trunk mutation in the primary autochthonous cancer and binds with highest affinity to the MHC. A high-avidity mp68-specific TCR was used to genetically engineer T cells as well as to generate TCR-transgenic mice for adoptive therapy. When the neoepitope was expressed at high levels and by all cancer cells, their direct recognition sufficed to destroy intratumor vessels and eradicate large, long-established solid tumors. When the neoepitope was targeted as autochthonous antigen, T cells caused cancer regression followed by escape of antigen-negative variants. Escape could be thwarted by expressing the antigen at increased levels in all cancer cells or by combining T-cell therapy with local irradiation. Therapeutic efficacies of TCR-transduced and TCR-transgenic T cells were similar. Gene therapy with a single TCR targeting a single AAS can eradicate large established cancer, but a uniform expression and/or sufficient levels of the targeted neoepitope or additional therapy are required to overcome tumor escape. Clin Cancer Res; 22(11); 2734-43. ©2015 AACRSee related commentary by Liu, p. 2602. ©2015 American Association for Cancer Research.

Dual targeting of glioblastoma with chimeric antigen receptor-engineered natural killer cells overcomes heterogeneity of target antigen expression and enhances antitumor activity and survival.

PubMed

Genßler, Sabrina; Burger, Michael C; Zhang, Congcong; Oelsner, Sarah; Mildenberger, Iris; Wagner, Marlies; Steinbach, Joachim P; Wels, Winfried S

2016-04-01

Epidermal growth factor receptor (EGFR) and its mutant form EGFRvIII are overexpressed in a large proportion of glioblastomas (GBM). Immunotherapy with an EGFRvIII-specific vaccine has shown efficacy against GBM in clinical studies. However, immune escape by antigen-loss variants and lack of control of EGFR wild-type positive clones limit the usefulness of this approach. Chimeric antigen receptor (CAR)-engineered natural killer (NK) cells may represent an alternative immunotherapeutic strategy. For targeting to GBM, we generated variants of the clinically applicable human NK cell line NK-92 that express CARs carrying a composite CD28-CD3ζ domain for signaling, and scFv antibody fragments for cell binding either recognizing EGFR, EGFRvIII, or an epitope common to both antigens. In vitro analysis revealed high and specific cytotoxicity of EGFR-targeted NK-92 against established and primary human GBM cells, which was dependent on EGFR expression and CAR signaling. EGFRvIII-targeted NK-92 only lysed EGFRvIII-positive GBM cells, while dual-specific NK cells expressing a cetuximab-based CAR were active against both types of tumor cells. In immunodeficient mice carrying intracranial GBM xenografts either expressing EGFR, EGFRvIII or both receptors, local treatment with dual-specific NK cells was superior to treatment with the corresponding monospecific CAR NK cells. This resulted in a marked extension of survival without inducing rapid immune escape as observed upon therapy with monospecific effectors. Our results demonstrate that dual targeting of CAR NK cells reduces the risk of immune escape and suggest that EGFR/EGFRvIII-targeted dual-specific CAR NK cells may have potential for adoptive immunotherapy of glioblastoma.

Landscape-scale distribution and persistence of genetically modified oilseed rape (Brassica napus) in Manitoba, Canada.

PubMed

Knispel, Alexis L; McLachlan, Stéphane M

2010-01-01

Genetically modified herbicide-tolerant (GMHT) oilseed rape (OSR; Brassica napus L.) was approved for commercial cultivation in Canada in 1995 and currently represents over 95% of the OSR grown in western Canada. After a decade of widespread cultivation, GMHT volunteers represent an increasing management problem in cultivated fields and are ubiquitous in adjacent ruderal habitats, where they contribute to the spread of transgenes. However, few studies have considered escaped GMHT OSR populations in North America, and even fewer have been conducted at large spatial scales (i.e. landscape scales). In particular, the contribution of landscape structure and large-scale anthropogenic dispersal processes to the persistence and spread of escaped GMHT OSR remains poorly understood. We conducted a multi-year survey of the landscape-scale distribution of escaped OSR plants adjacent to roads and cultivated fields. Our objective was to examine the long-term dynamics of escaped OSR at large spatial scales and to assess the relative importance of landscape and localised factors to the persistence and spread of these plants outside of cultivation. From 2005 to 2007, we surveyed escaped OSR plants along roadsides and field edges at 12 locations in three agricultural landscapes in southern Manitoba where GMHT OSR is widely grown. Data were analysed to examine temporal changes at large spatial scales and to determine factors affecting the distribution of escaped OSR plants in roadside and field edge habitats within agricultural landscapes. Additionally, we assessed the potential for seed dispersal between escaped populations by comparing the relative spatial distribution of roadside and field edge OSR. Densities of escaped OSR fluctuated over space and time in both roadside and field edge habitats, though the proportion of GMHT plants was high (93-100%). Escaped OSR was positively affected by agricultural landscape (indicative of cropping intensity) and by the presence of an adjacent field planted to OSR. Within roadside habitats, escaped OSR was also strongly associated with large-scale variables, including road surface (indicative of traffic intensity) and distance to the nearest grain elevator. Conversely, within field edges, OSR density was affected by localised crop management practices such as mowing, soil disturbance and herbicide application. Despite the proximity of roadsides and field edges, there was little evidence of spatial aggregation among escaped OSR populations in these two habitats, especially at very fine spatial scales (i.e. <100 m), suggesting that natural propagule exchange is infrequent. Escaped OSR populations were persistent at large spatial and temporal scales, and low density in a given landscape or year was not indicative of overall extinction. As a result of ongoing cultivation and transport of OSR crops, escaped GMHT traits will likely remain predominant in agricultural landscapes. While escaped OSR in field edge habitats generally results from local seeding and management activities occurring at the field-scale, distribution patterns within roadside habitats are determined in large part by seed transport occurring at the landscape scale and at even larger regional scales. Our findings suggest that these large-scale anthropogenic dispersal processes are sufficient to enable persistence despite limited natural seed dispersal. This widespread dispersal is likely to undermine field-scale management practices aimed at eliminating escaped and in-field GMHT OSR populations. Agricultural transport and landscape-scale cropping patterns are important determinants of the distribution of escaped GM crops. At the regional level, these factors ensure ongoing establishment and spread of escaped GMHT OSR despite limited local seed dispersal. Escaped populations thus play an important role in the spread of transgenes and have substantial implications for the coexistence of GM and non-GM production systems. Given the large-scale factors driving the spread of escaped transgenes, localised co-existence measures may be impracticable where they are not commensurate with regional dispersal mechanisms. To be effective, strategies aimed at reducing contamination from GM crops should be multi-scale in approach and be developed and implemented at both farm and landscape levels of organisation. Multiple stakeholders should thus be consulted, including both GM and non-GM farmers, as well as seed developers, processors, transporters and suppliers. Decisions to adopt GM crops require thoughtful and inclusive consideration of the risks and responsibilities inherent in this new technology.

33 CFR 385.13 - Projects implemented under additional program authority.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Implementation Report is prepared and approved in accordance with § 385.26; and (3) Not exceed a total cost of... RESTORATION PLAN CERP Implementation Processes § 385.13 Projects implemented under additional program authority. (a) To expedite implementation of the Plan, the Corps of Engineers and non-Federal sponsors may...

The DYNAMO Orbiter Project: High Resolution Mapping of Gravity/Magnetic Fields and In Situ Investigation of Mars Atmospheric Escape

NASA Technical Reports Server (NTRS)

Smrekar, S.; Chassefiere, E.; Forget, F.; Reme, H.; Mazelle, C.; Blelly, P. -L.; Acuna, M.; Connerney, J.; Purucker, M.; Lin, R.

2000-01-01

Dynamo is a small Mars orbiter planned to be launched in 2005 or 2007, in the frame of the NASA/CNES Mars exploration program. It is aimed at improving gravity and magnetic field resolution, in order to better understand the magnetic, geologic and thermal history of Mars, and at characterizing current atmospheric escape, which is still poorly constrained. These objectives are achieved by using a low periapsis orbit, similar to the one used by the Mars Global Surveyor spacecraft during its aerobraking phases. The proposed periapsis altitude for Dynamo of 120-130 km, coupled with the global distribution of periapses to be obtained during one Martian year of operation, through about 5000 low passes, will produce a magnetic/gravity field data set with approximately five times the spatial resolution of MGS. Low periapsis provides a unique opportunity to investigate the chemical and dynamical properties of the deep ionosphere, thermosphere, and the interaction between the atmosphere and the solar wind, therefore atmospheric escape, which may have played a crucial role in removing atmosphere, and water, from the planet. There is much room for debate on the importance of current atmosphere escape processes in the evolution of the Martian atmosphere, as early "exotic" processes including hydrodynamic escape and impact erosion are traditionally invoked to explain the apparent sparse inventory of present-day volatiles. Yet, the combination of low surface gravity and the absence of a substantial internally generated magnetic field have undeniable effects on what we observe today. In addition to the current losses in the forms of Jeans and photochemical escape of neutrals, there are solar wind interaction-related erosion mechanisms because the upper atmosphere is directly exposed to the solar wind. The solar wind related loss rates, while now comparable to those of a modest comet, nonetheless occur continuously, with the intriguing possibility of important cumulative and/or enhanced effects over the several billion years of the solar system's life. If the detailed history of the Martian internal field could be traced back, and the current escape processes could be understood well enough to model the expected stronger losses under early Sun conditions, one could go a long way toward constraining this part of the mysterious history of Mars' atmosphere.

Macrophage-mediated trogocytosis leads to death of antibody-opsonized tumor cells

PubMed Central

Velmurugan, Ramraj; Challa, Dilip K.; Ram, Sripad; Ober, Raimund J.; Ward, E. Sally

2016-01-01

Understanding the complex behavior of effector cells such as monocytes or macrophages in regulating cancerous growth is of central importance for cancer immunotherapy. Earlier studies using CD20-specific antibodies have demonstrated that the Fcγ receptor (FcγR)-mediated transfer of the targeted receptors from tumor cells to these effector cells through trogocytosis can enable escape from antibody therapy, leading to the viewpoint that this process is pro-tumorigenic. In the current study we demonstrate that persistent trogocytic attack results in the killing of HER2-overexpressing breast cancer cells. Further, antibody engineering to increase FcγR interactions enhances this tumoricidal activity. These studies extend the complex repertoire of activities of macrophages to trogocytic-mediated cell death of HER2-overexpressing target cells and have implications for the development of effective antibody-based therapies. PMID:27226489

Comparison of catch per unit effort among four minnow trap models in the three-spined stickleback (Gasterosteus aculeatus) fishery.

PubMed

Budria, Alexandre; DeFaveri, Jacquelin; Merilä, Juha

2015-12-21

Minnow traps are commonly used in the stickleback (Gasterostidae) fishery, but the potential differences in catch per unit effort (CPUE) among different minnow trap models are little studied. We compared the CPUE of four different minnow trap models in field experiments conducted with three-spined sticklebacks (Gasterosteus aculeatus). Marked (up to 26 fold) differences in median CPUE among different trap models were observed. Metallic uncoated traps yielded the largest CPUE (2.8 fish/h), followed by metallic black nylon-coated traps (1.3 fish/h). Collapsible canvas traps yielded substantially lower CPUEs (black: 0.7 fish/h; red: 0.1 fish/h) than the metallic traps. Laboratory trials further revealed significant differences in escape probabilities among the different trap models. While the differences in escape probability can explain at least part of the differences in CPUE among the trap models (e.g. high escape rate and low CPUE in red canvas traps), discrepancies between model-specific CPUEs and escape rates suggests that variation in entrance rate also contributes to the differences in CPUE. In general, and in accordance with earlier data on nine-spined stickleback (Pungitius pungitius) trapping, the results suggest that uncoated metallic (Gee-type) traps are superior to the other commonly used minnow trap models in stickleback fisheries.

Comparison of catch per unit effort among four minnow trap models in the three-spined stickleback (Gasterosteus aculeatus) fishery

PubMed Central

Budria, Alexandre; DeFaveri, Jacquelin; Merilä, Juha

2015-01-01

Minnow traps are commonly used in the stickleback (Gasterostidae) fishery, but the potential differences in catch per unit effort (CPUE) among different minnow trap models are little studied. We compared the CPUE of four different minnow trap models in field experiments conducted with three-spined sticklebacks (Gasterosteus aculeatus). Marked (up to 26 fold) differences in median CPUE among different trap models were observed. Metallic uncoated traps yielded the largest CPUE (2.8 fish/h), followed by metallic black nylon-coated traps (1.3 fish/h). Collapsible canvas traps yielded substantially lower CPUEs (black: 0.7 fish/h; red: 0.1 fish/h) than the metallic traps. Laboratory trials further revealed significant differences in escape probabilities among the different trap models. While the differences in escape probability can explain at least part of the differences in CPUE among the trap models (e.g. high escape rate and low CPUE in red canvas traps), discrepancies between model-specific CPUEs and escape rates suggests that variation in entrance rate also contributes to the differences in CPUE. In general, and in accordance with earlier data on nine-spined stickleback (Pungitius pungitius) trapping, the results suggest that uncoated metallic (Gee-type) traps are superior to the other commonly used minnow trap models in stickleback fisheries. PMID:26685761

Distribution of escaping ions produced by non-specular reflection at the stationary quasi-perpendicular shock front

NASA Astrophysics Data System (ADS)

Gedalin, M.; Liverts, M.; Balikhin, M. A.

2008-05-01

Field-aligned and gyrophase bunched ion beams are observed in the foreshock of the Earth bow shock. One of the mechanisms proposed for their production is non-specular reflection at the shock front. We study the distributions which are formed at the stationary quasi-perpendicular shock front within the same process which is responsible for the generation of reflected ions and transmitted gyrating ions. The test particle motion analysis in a model shock allows one to identify the parameters which control the efficiency of the process and the features of the escaping ion distribution. These parameters are: the angle between the shock normal and the upstream magnetic field, the ratio of the ion thermal velocity to the flow velocity upstream, and the cross-shock potential. A typical distribution of escaping ions exhibits a bimodal pitch angle distribution (in the plasma rest frame).

Mars Atmospheric Escape Recorded by H, C and O Isotope Ratios in Carbon Dioxide and Water Measured by the Sam Tunable Laser Spectrometer on the Curiosity Rover

NASA Technical Reports Server (NTRS)

Webster, C. R.; Mahaffy, P. R.; Leshin, L. A.; Atreya, S. K.; Flesch, G. J.; Stern, J.; Christensen, L. E.; Vasavada, A. R.; Owen, T.; Niles, P. B.;

Towards a Genetic Definition of Cancer-Associated Inflammation

PubMed Central

Prendergast, George C.; Metz, Richard; Muller, Alexander J.

2010-01-01

Chronic inflammation drives the development of many cancers, but a genetic definition of what constitutes ‘cancer-associated’ inflammation has not been determined. Recently, a mouse genetic study revealed a critical role for the immune escape mediator indoleamine 2,3-dioxygenase (IDO) in supporting inflammatory skin carcinogenesis. IDO is generally regarded as being immunosuppressive; however, there was no discernable difference in generalized inflammatory processes in IDO-null mice under conditions where tumor development was significantly suppressed, implicating IDO as key to establishing the pathogenic state of ‘cancer-associated’ inflammation. Here we review recent findings and their potential implications to understanding the relationship between immune escape and inflammation in cancer. Briefly, we propose that genetic pathways of immune escape in cancer are synonymous with pathways that define ‘cancer-associated’ inflammation and that these processes may be identical rather than distinct, as generally presumed, in terms of their genetic definition. PMID:20228228

Propulsion and Energetics Panel Working Group 13 on Alternative Jet Engine Fuels. Volume 2. Main Report

DTIC Science & Technology

1982-07-01

twenty years the only economically available fuels for aircraft gas turbine engines will be those from the processing of conventional crude petroleum...alternative fuels in new aircraft engines. i.e. problems ir, combustors. turbines . and afterburners. and methods for their solution. - Fuel system...required expertise assigned to each task group. The three areas were. Supply and demand scenarios for aviation turbine fuels in the NATO Nations for the

Conflicting Selection Pressures Will Constrain Viral Escape from Interfering Particles: Principles for Designing Resistance-Proof Antivirals.

PubMed

Rast, Luke I; Rouzine, Igor M; Rozhnova, Ganna; Bishop, Lisa; Weinberger, Ariel D; Weinberger, Leor S

2016-05-01

The rapid evolution of RNA-encoded viruses such as HIV presents a major barrier to infectious disease control using conventional pharmaceuticals and vaccines. Previously, it was proposed that defective interfering particles could be developed to indefinitely control the HIV/AIDS pandemic; in individual patients, these engineered molecular parasites were further predicted to be refractory to HIV's mutational escape (i.e., be 'resistance-proof'). However, an outstanding question has been whether these engineered interfering particles-termed Therapeutic Interfering Particles (TIPs)-would remain resistance-proof at the population-scale, where TIP-resistant HIV mutants may transmit more efficiently by reaching higher viral loads in the TIP-treated subpopulation. Here, we develop a multi-scale model to test whether TIPs will maintain indefinite control of HIV at the population-scale, as HIV ('unilaterally') evolves toward TIP resistance by limiting the production of viral proteins available for TIPs to parasitize. Model results capture the existence of two intrinsic evolutionary tradeoffs that collectively prevent the spread of TIP-resistant HIV mutants in a population. First, despite their increased transmission rates in TIP-treated sub-populations, unilateral TIP-resistant mutants are shown to have reduced transmission rates in TIP-untreated sub-populations. Second, these TIP-resistant mutants are shown to have reduced growth rates (i.e., replicative fitness) in both TIP-treated and TIP-untreated individuals. As a result of these tradeoffs, the model finds that TIP-susceptible HIV strains continually outcompete TIP-resistant HIV mutants at both patient and population scales when TIPs are engineered to express >3-fold more genomic RNA than HIV expresses. Thus, the results provide design constraints for engineering population-scale therapies that may be refractory to the acquisition of antiviral resistance.

Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sung, Min Sun; Biosystems and Bioengineering Program, University of Science and Technology; Mun, Ji-Young

2013-07-19

Highlights: •MyoD was engineered to contain protein transduction domain and endosome-disruptive INF7 peptide. •The engineered MyoD-IT showed efficient nuclear targeting through an endosomal escape by INF7 peptide. •By applying MyoD-IT, human adipose-derived stem cells (hASCs) were differentiated into myogenic cells. •hASCs differentiated by applying MyoD-IT fused to myotubes through co-culturing with mouse myoblasts. •Myogenic differentiation using MyoD-IT is a safe method without the concern of altering the genome. -- Abstract: Human adipose-derived stem cells (hASCs) have great potential as cell sources for the treatment of muscle disorders. To provide a safe method for the myogenic differentiation of hASCs, we engineeredmore » the MyoD protein, a key transcription factor for myogenesis. The engineered MyoD (MyoD-IT) was designed to contain the TAT protein transduction domain for cell penetration and the membrane-disrupting INF7 peptide, which is an improved version of the HA2 peptide derived from influenza. MyoD-IT showed greatly improved nuclear targeting ability through an efficient endosomal escape induced by the pH-sensitive membrane disruption of the INF7 peptide. By applying MyoD-IT to a culture, hASCs were efficiently differentiated into long spindle-shaped myogenic cells expressing myosin heavy chains. Moreover, these cells differentiated by an application of MyoD-IT fused to myotubes with high efficiency through co-culturing with mouse C2C12 myoblasts. Because internalized proteins can be degraded in cells without altering the genome, the myogenic differentiation of hASCs using MyoD-IT would be a safe and clinically applicable method.« less

Direct activation of the Mauthner cell by electric field pulses drives ultrarapid escape responses

PubMed Central

Tabor, Kathryn M.; Bergeron, Sadie A.; Horstick, Eric J.; Jordan, Diana C.; Aho, Vilma; Porkka-Heiskanen, Tarja; Haspel, Gal

2014-01-01

Rapid escape swims in fish are initiated by the Mauthner cells, giant reticulospinal neurons with unique specializations for swift responses. The Mauthner cells directly activate motoneurons and facilitate predator detection by integrating acoustic, mechanosensory, and visual stimuli. In addition, larval fish show well-coordinated escape responses when exposed to electric field pulses (EFPs). Sensitization of the Mauthner cell by genetic overexpression of the voltage-gated sodium channel SCN5 increased EFP responsiveness, whereas Mauthner ablation with an engineered variant of nitroreductase with increased activity (epNTR) eliminated the response. The reaction time to EFPs is extremely short, with many responses initiated within 2 ms of the EFP. Large neurons, such as Mauthner cells, show heightened sensitivity to extracellular voltage gradients. We therefore tested whether the rapid response to EFPs was due to direct activation of the Mauthner cells, bypassing delays imposed by stimulus detection and transmission by sensory cells. Consistent with this, calcium imaging indicated that EFPs robustly activated the Mauthner cell but only rarely fired other reticulospinal neurons. Further supporting this idea, pharmacological blockade of synaptic transmission in zebrafish did not affect Mauthner cell activity in response to EFPs. Moreover, Mauthner cells transgenically expressing a tetrodotoxin (TTX)-resistant voltage-gated sodium channel retained responses to EFPs despite TTX suppression of action potentials in the rest of the brain. We propose that EFPs directly activate Mauthner cells because of their large size, thereby driving ultrarapid escape responses in fish. PMID:24848468

46 CFR 71.65-5 - Plans and specifications required for new construction.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) [Reserved] (h) Crew's accommodations. (1) Arrangement plans showing accommodations, ventilation, escapes... Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The principal details of... stability. Plans and calculations required by subchapter S of this chapter. (d) Fire control. (1) Fire...

32 CFR 644.450 - Items excluded from usual restoration obligation.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., ventilators, and metal ceilings. (8) Structural steel or iron. (9) Fire escapes. (10) Heating systems. (11) Plumbing systems. (12) Ventilating systems and air conditioning systems. (13) Power plants. (14) Electric wiring. (15) Lighting fixtures (or replacement). (16) Sprinkler systems. (f) Settling or subsidence. (g...

32 CFR 644.450 - Items excluded from usual restoration obligation.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., ventilators, and metal ceilings. (8) Structural steel or iron. (9) Fire escapes. (10) Heating systems. (11) Plumbing systems. (12) Ventilating systems and air conditioning systems. (13) Power plants. (14) Electric wiring. (15) Lighting fixtures (or replacement). (16) Sprinkler systems. (f) Settling or subsidence. (g...

32 CFR 644.450 - Items excluded from usual restoration obligation.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., ventilators, and metal ceilings. (8) Structural steel or iron. (9) Fire escapes. (10) Heating systems. (11) Plumbing systems. (12) Ventilating systems and air conditioning systems. (13) Power plants. (14) Electric wiring. (15) Lighting fixtures (or replacement). (16) Sprinkler systems. (f) Settling or subsidence. (g...

46 CFR 12.15-13 - Deck engine mechanic.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Deck engine mechanic. 12.15-13 Section 12.15-13 Shipping... ENDORSEMENTS Qualified Member of the Engine Department § 12.15-13 Deck engine mechanic. (a) An applicant for an endorsement as deck engine mechanic shall be a person holding an MMC or MMD endorsed as junior engineer. The...

46 CFR 12.15-13 - Deck engine mechanic.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 1 2012-10-01 2012-10-01 false Deck engine mechanic. 12.15-13 Section 12.15-13 Shipping... ENDORSEMENTS Qualified Member of the Engine Department § 12.15-13 Deck engine mechanic. (a) An applicant for an endorsement as deck engine mechanic shall be a person holding an MMC or MMD endorsed as junior engineer. The...

46 CFR 12.15-13 - Deck engine mechanic.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 1 2013-10-01 2013-10-01 false Deck engine mechanic. 12.15-13 Section 12.15-13 Shipping... ENDORSEMENTS Qualified Member of the Engine Department § 12.15-13 Deck engine mechanic. (a) An applicant for an endorsement as deck engine mechanic shall be a person holding an MMC or MMD endorsed as junior engineer. The...

46 CFR 12.15-13 - Deck engine mechanic.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Deck engine mechanic. 12.15-13 Section 12.15-13 Shipping... ENDORSEMENTS Qualified Member of the Engine Department § 12.15-13 Deck engine mechanic. (a) An applicant for an endorsement as deck engine mechanic shall be a person holding an MMC or MMD endorsed as junior engineer. The...

Can we stop transgenes from taking a walk on the wild side?

PubMed

Dlugosch, Katrina M; Whitton, Jeannette

2008-03-01

Whether the potential costs associated with broad-scale use of genetically modified organisms (GMOs) outweigh possible benefits is highly contentious, including within the scientific community. Even among those generally in favour of commercialization of GM crops, there is nonetheless broad recognition that transgene escape into the wild should be minimized. But is it possible to achieve containment of engineered genetic elements in the context of large scale agricultural production? In a previous study, Warwick et al. (2003) documented transgene escape via gene flow from herbicide resistant (HR) canola (Brassica napus) into neighbouring weedy B. rapa populations (Fig. 1) in two agricultural fields in Quebec, Canada. In a follow-up study in this issue of Molecular Ecology, Warwick et al. (2008) show that the transgene has persisted and spread within the weedy population in the absence of selection for herbicide resistance. Certainly a trait like herbicide resistance is expected to spread when selected through the use of the herbicide, despite potentially negative epistatic effects on fitness. However, Warwick et al.'s findings suggest that direct selection favouring the transgene is not required for its persistence. So is there any hope of preventing transgene escape into the wild?

34 CFR Appendix to Part 648 - Academic Areas

Code of Federal Regulations, 2011 CFR

2011-07-01

... Administration and Supervision 13.05Educational/Instructional Media Design 13.06Educational Evaluation, Research, and Statistics 13.07International and Comparative Education 13.08Educational Psychology 13.09Social....27Systems Engineering 14.28Textile Sciences and Engineering 14.29Engineering Design 14.30Engineering...

34 CFR Appendix to Part 648 - Academic Areas

Code of Federal Regulations, 2012 CFR

2012-07-01

... Administration and Supervision 13.05Educational/Instructional Media Design 13.06Educational Evaluation, Research, and Statistics 13.07International and Comparative Education 13.08Educational Psychology 13.09Social....27Systems Engineering 14.28Textile Sciences and Engineering 14.29Engineering Design 14.30Engineering...

34 CFR Appendix to Part 648 - Academic Areas

Code of Federal Regulations, 2014 CFR

2014-07-01

... Administration and Supervision 13.05Educational/Instructional Media Design 13.06Educational Evaluation, Research, and Statistics 13.07International and Comparative Education 13.08Educational Psychology 13.09Social....27Systems Engineering 14.28Textile Sciences and Engineering 14.29Engineering Design 14.30Engineering...

34 CFR Appendix to Part 648 - Academic Areas

Code of Federal Regulations, 2013 CFR

2013-07-01

... Administration and Supervision 13.05Educational/Instructional Media Design 13.06Educational Evaluation, Research, and Statistics 13.07International and Comparative Education 13.08Educational Psychology 13.09Social....27Systems Engineering 14.28Textile Sciences and Engineering 14.29Engineering Design 14.30Engineering...

Effect of interface-dependent crystalline boundary on sub-threshold characteristics in a solution-processed 6,13-bis(triisopropylsilylethynyl)-pentacene thin-film transistor

NASA Astrophysics Data System (ADS)

Kwon, Jin-Hyuk; Kang, In Man; Bae, Jin-Hyuk

2014-03-01

We demonstrate how the sub-threshold characteristics are affected by the density of crystalline domain boundaries directly governed by an organic semiconductor (OSC) - a gate insulator interface in a solution-processed 6,13-bis(triisopropylsilylethynyl)-pentacene (TIPS-pentacene) thin-film transistor (TFT). For generation of an engineered interface, a self assembled monolayer of octadecyltricholorosilane (OTS) was produced between a solution processed TIPS-pentacene film and a silicon dioxide layer. The interfacial charge trap density (Ntrap) deduced from the sub-threshold characteristics was significantly minimized after OTS treatment due to reduced crystal domain boundaries in the TIPS-pentacene film. In addition, the carrier mobility exhibits a value twice as large by OTS treatment. It is found that less crystal domain boundaries in the solution-processed OSC obtained from the engineered interface play an important role in inducing improved sub-threshold characteristics together with increased carrier mobility in organic TFTs.

3 CFR 8639 - Proclamation 8639 of March 24, 2011. 100th Anniversary of the Triangle Shirtwaist Factory Fire

Code of Federal Regulations, 2012 CFR

2012-01-01

... buckets of water to douse the flames—giving the factory workers there little time to escape. When the..., and uphold collective bargaining as a tool to give workers a seat at the tables of power. Working Americans are the backbone of our communities and power the engine of our economy. As we mark the...

Can the University Escape from the Labyrinth of Technology? Part 4: Extending the Strategy to Medicine, the Social Sciences, and the University

ERIC Educational Resources Information Center

Vanderburg, Willem H.

2006-01-01

This fourth part outlines a strategy for overcoming the limitations of the knowledge system for engineering by combining intellectual maps, preventive approaches, umbrella concepts, and round tables as described in the earlier parts. A discussion of the issues faced by modern medicine illustrates the paradigmatic nature of the diagnosis and…

Mechanisms of Ionospheric Mass Escape

NASA Technical Reports Server (NTRS)

Moore, T. E.; Khazanov, G. V.

2010-01-01

The dependence of ionospheric O+ escape flux on electromagnetic energy flux and electron precipitation into the ionosphere is derived for a hypothetical ambipolar pick-up process, powered the relative motion of plasmas and neutral upper atmosphere, and by electron precipitation, at heights where the ions are magnetized but influenced by photo-ionization, collisions with gas atoms, ambipolar and centrifugal acceleration. Ion pick-up by the convection electric field produces "ring-beam" or toroidal velocity distributions, as inferred from direct plasma measurements, from observations of the associated waves, and from the spectra of incoherent radar echoes. Ring-beams are unstable to plasma wave growth, resulting in rapid relaxation via transverse velocity diffusion, into transversely accelerated ion populations. Ion escape is substantially facilitated by the ambipolar potential, but is only weakly affected by centrifugal acceleration. If, as cited simulations suggest, ion ring beams relax into non-thermal velocity distributions with characteristic speed equal to the local ion-neutral flow speed, a generalized "Jeans escape" calculation shows that the escape flux of ionospheric O+ increases with Poynting flux and with precipitating electron density in rough agreement with observations.

RNA virus interference via CRISPR/Cas13a system in plants.

PubMed

Aman, Rashid; Ali, Zahir; Butt, Haroon; Mahas, Ahmed; Aljedaani, Fatimah; Khan, Muhammad Zuhaib; Ding, Shouwei; Mahfouz, Magdy

2018-01-04

CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants. CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana. CRISPR RNA (crRNAs) targeting the HC-Pro and GFP sequences exhibit better interference than those targeting other regions such as coat protein (CP) sequence. Cas13a can also process pre-crRNAs into functional crRNAs. Our data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity against RNA viruses and for other RNA manipulations in plants.

Thermal escape from extrasolar giant planets

PubMed Central

Koskinen, Tommi T.; Lavvas, Panayotis; Harris, Matthew J.; Yelle, Roger V.

2014-01-01

The detection of hot atomic hydrogen and heavy atoms and ions at high altitudes around close-in extrasolar giant planets (EGPs) such as HD209458b implies that these planets have hot and rapidly escaping atmospheres that extend to several planetary radii. These characteristics, however, cannot be generalized to all close-in EGPs. The thermal escape mechanism and mass loss rate from EGPs depend on a complex interplay between photochemistry and radiative transfer driven by the stellar UV radiation. In this study, we explore how these processes change under different levels of irradiation on giant planets with different characteristics. We confirm that there are two distinct regimes of thermal escape from EGPs, and that the transition between these regimes is relatively sharp. Our results have implications for thermal mass loss rates from different EGPs that we discuss in the context of currently known planets and the detectability of their upper atmospheres. PMID:24664923

Studying Lyman-alpha escape and reionization in Green Pea galaxies

NASA Astrophysics Data System (ADS)

Yang, Huan; Malhotra, Sangeeta; Rhoads, James E.; Gronke, Max; Leitherer, Claus; Wofford, Aida; Dijkstra, Mark

2017-01-01

Green Pea galaxies are low-redshift galaxies with extreme [OIII]5007 emission line. We built the first statistical sample of Green Peas observed by HST/COS and used them as analogs of high-z Lyman-alpha emitters to study Ly-alpha escape and Ly-alpha sizes. Using the HST/COS 2D spectra, we found that Ly-alpha sizes of Green Peas are larger than the UV continuum sizes. We found many correlations between Ly-alpha escape fraction and galactic properties -- dust extinction, Ly-alpha kinematic features, [OIII]/[OII] ratio, and gas outflow velocities. We fit an empirical relation to predict Ly-alpha escape fraction from dust extinction and Ly-alpha red-peak velocity. In the JWST era, we can use this relation to derive the IGM HI column density along the line of sight of each high-z Ly-alpha emitter and probe the reionization process.

Thermal escape from extrasolar giant planets.

PubMed

Koskinen, Tommi T; Lavvas, Panayotis; Harris, Matthew J; Yelle, Roger V

2014-04-28

The detection of hot atomic hydrogen and heavy atoms and ions at high altitudes around close-in extrasolar giant planets (EGPs) such as HD209458b implies that these planets have hot and rapidly escaping atmospheres that extend to several planetary radii. These characteristics, however, cannot be generalized to all close-in EGPs. The thermal escape mechanism and mass loss rate from EGPs depend on a complex interplay between photochemistry and radiative transfer driven by the stellar UV radiation. In this study, we explore how these processes change under different levels of irradiation on giant planets with different characteristics. We confirm that there are two distinct regimes of thermal escape from EGPs, and that the transition between these regimes is relatively sharp. Our results have implications for thermal mass loss rates from different EGPs that we discuss in the context of currently known planets and the detectability of their upper atmospheres.

The Development of Relevant Indicators for Planning, Monitoring and Evaluation of Country Efforts for Promoting Youth's Role in Development. Report of the Expert Group Meeting (Manila, Philippines, December 13-20, 1980).

ERIC Educational Resources Information Center

United Nations Economic and Social Commission for Asia and the Pacific, Bangkok (Thailand).

The report of a United Nations Economic and Social Commission for Asia and the Pacific (ESCAP) Expert Group meeting, involving 13 experts from 10 countries, discusses planning national efforts to promote youth's role in development. Current systems and indicators used to assess the situation of rural and urban youth and their contribution to…

Critical Materials Needs

DTIC Science & Technology

1975-08-11

Desulfurization of flue gases from electric power plants Arthur J. Coyle Walter E. Chapin John B. Day John T. Herridge Victor Levin James...45 High-Temperature Gas -Turbine Engines for Automotive Applications 60 Fuel Cel13 76 Lasers for Communications and Materials Processing 97...Relationship for a Regenerative Gas -Turbine Engine 61 Relative Raw Materials Cost 61 Proposed Milestone Chart ERDA/AAPS Ceramic Mate- rials and

13th Annual Systems Engineering Conference: Tues- Wed

DTIC Science & Technology

2010-10-28

greater understanding/documentation of lessons learned – Promotes SE within the organization • Justification for continued funding of SE Infrastructure...educational process – Addresses the development of innovative learning tools, strategies, and teacher training • Research and Development – Promotes ...technology, and mathematics • More commitment to engaging young students in science, engineering, technology and mathematics • More rigor in defining

Comparing the Atmospheric Losses at Io and Europa

NASA Astrophysics Data System (ADS)

Dols, V. J.; Bagenal, F.; Crary, F. J.; Cassidy, T.

2017-12-01

At Io and Europa, the interaction of the Jovian plasma with the moon atmosphere leads to a significant loss of atomic/molecular neutrals and ions to space. The processes that lead to atmospheric escape are diverse: atmospheric sputtering, molecular dissociation, molecular ion recombination, Jeans escape etc. Each process leads to neutrals escaping at different velocities (i.e. electron impact dissociation leads to very slow atomic neutrals, sputtering might eject faster molecular neutrals). Some neutrals will be ejected out of the Jovian system; others will form extended neutral clouds along the orbit of the moons. These atomic/molecular extended neutral clouds are probably the main source of plasma for the Jovian magnetosphere. They are difficult to observe directly thus their composition and density are still poorly constrained. A future modeling of the formation of these extended clouds requires an estimate of their atmospheric sources. We estimate the atmospheric losses at Io and Europa for each loss process with a multi-species chemistry model, using a prescribed atmospheric distribution consistent with the observations. We compare the neutral losses at Io and Europa.

Associations of Neuroticism-Impulsivity and Coping with Binge Eating in a Nationally Representative Sample of Adolescents in the United States

PubMed Central

Townsend, Lisa; Reinblatt, Shauna P.; Mendelson, Tamar

2016-01-01

Objective Binge eating behavior is a public health concern due to its negative physical and mental health consequences. Little is known about the interplay of personality traits, coping styles, and binge eating in the general adolescent population. We examined the associations among the combination of neuroticism and impulsivity (NI), maladaptive coping styles (poor problem solving, distraction, and escape-avoidance), and lifetime prevalence of binge eating in a nationally representative sample of U.S. adolescents. We also explored coping as a moderator of the NI–lifetime binge eating association and gender as a moderator of the NI–coping associations and coping–lifetime binge eating associations. Methods We used data from the National Comorbidity Survey: Adolescent Supplement (NCS-A: 2001–2004), a cross-sectional nationally representative study of adolescents aged 13 to 18 years (n=10,028). We studied the associations of NI and coping with lifetime binge eating using multivariate regression models. Results High NI was significantly associated with all three coping styles, especially escape-avoidance (β = 3.96, confidence interval [CI] = 3.62, 4.29, p < 0.001). Gender was a significant moderator of the NI–distraction coping association (β = −0.68, CI = −1.33, −0.03, p = 0.041), indicating a stronger association in males (β = 1.20, CI = 0.81, 1.58, p < 0.001) than females (β = 0.53, CI = 0.02, 1.03, p = 0.042). Lifetime prevalence of binge eating was 1.13 times higher with increased escape-avoidance coping (CI = 1.10, 1.18, p < 0.001). Discussion Our findings indicate significant associations among high NI, increased escape-avoidance coping, and higher lifetime prevalence of binge eating in adolescents. Findings of our study have potential to inform development of interventions that target modification of maladaptive personality traits and coping styles to reduce problematic eating. PMID:27289518

Army Research Office and Air Force Office of Scientific Research Contractors’ Meeting in Chemical Propulsion Held in Boulder, Colorado on June 11-13, 2007

DTIC Science & Technology

2007-06-01

single-cylinder diesel engine showed that at high EGR rates, the autoignition mechanism changes from the typical single stage to a two-stage process...2002). 3. Edwards, T., Zabarnick, S. "Supercritical Fuel Deposition Mechanisms ," Industrial and Engineering Chemistry Research 32: 3117-3122 (1993...and the Air Force Office of Scientific Research. 20070910360 15. SUBJECT TERMS Flames, Propulsion, Gas Turbines, Diesel Engines , Scramjets, Pulse

Saturn Apollo Program

NASA Image and Video Library

1967-01-01

This cutaway illustration shows the Saturn V S-IVB (third) stage with the callouts of its major components. When the S-II (second) stage of the powerful Saturn V rocket burnt out and was separated the remaining units approached orbit around the Earth. Injection into the desired orbit was attaineded as the S-IVB (third stage) was ignited and burnt. The S-IVB stage was powered by a single 200,000-pound thrust J-2 engine and had a re-start capability built in for its J-2 engine. The S-IVB restarted to speed the Apollo spacecraft to escape velocity injecting it and the astronauts into a moon trajectory.

Biotemplated Synthesis of PZT Nanowires

DTIC Science & Technology

2013-11-25

this same experimental protocol would be successful with other biotemplates. M13 bacteriophage was chosen as a second template because (1) it possesses...and (3) it can be customized to bind compounds of interest with tailorable specificities based on the phage display process.14 M13 is a virus that is...coefficients (d33 < 8 pm/V). 50 As negatively charged carboxyl groups can bind cations,51 M13 phages were genetically engineered to display glutamate

Lyman Continuum Escape Fraction of Star-forming Dwarf Galaxies at z ˜ 1

NASA Astrophysics Data System (ADS)

Rutkowski, Michael J.; Scarlata, Claudia; Haardt, Francesco; Siana, Brian; Henry, Alaina; Rafelski, Marc; Hayes, Matthew; Salvato, Mara; Pahl, Anthony J.; Mehta, Vihang; Beck, Melanie; Malkan, Matthew; Teplitz, Harry I.

2016-03-01

To date, no direct detection of Lyman continuum emission has been measured for intermediate-redshift (z˜ 1) star-forming galaxies. We combine Hubble Space Telescope grism spectroscopy with GALEX UV and ground-based optical imaging to extend the search for escaping Lyman continuum to a large (˜600) sample of z˜ 1 low-mass ({log}(\\bar{M}) ≃ 9.3{M}⊙ ), moderately star-forming (\\bar{{{\\Psi }}} ≲ 10{M}⊙ yr-1) galaxies selected initially on Hα emission. The characteristic escape fraction of LyC from star-forming galaxies (SFGs) that populate this parameter space remains weakly constrained by previous surveys, but these faint (sub-L⋆) SFGs are assumed to play a significant role in the reionization of neutral hydrogen in the intergalactic medium (IGM) at high redshift z\\gt 6. We do not make an unambiguous detection of escaping LyC radiation from this z˜ 1 sample, individual non-detections to constrain the absolute Lyman continuum escape fraction, {f}{esc} \\lt 2.1% (3σ). We measure an upper limit of {f}{esc} \\lt 9.6% from a sample of SFGs selected on high Hα equivalent width (EW \\gt 200 {{\\mathringA }}), which are thought to be close analogs of high redshift sources of reionization. For reference, we also present an emissivity-weighted escape fraction that is useful for measuring the general contribution SFGs to the ionizing UV background. In the discussion, we consider the implications of these intermediate redshift constraints for the reionization of hydrogen in the IGM at high (z\\gt 6) redshift. If we assume our z˜ 1 SFGs, for which we measure this emissivity-weighted {f}{esc}, are analogs to the high redshift sources of reionization, we find it is difficult to reconcile reionization by faint ({M}{UV}≲ -13) SFGs with a low escape fraction ({f}{esc} \\lt 3%), with constraints from independent high redshift observations. If {f}{esc} evolves with redshift, reionization by SFGs may be consistent with observations from Planck.

Escape of asteroids from the main belt

NASA Astrophysics Data System (ADS)

Granvik, Mikael; Morbidelli, Alessandro; Vokrouhlický, David; Bottke, William F.; Nesvorný, David; Jedicke, Robert

2017-02-01

Aims: We locate escape routes from the main asteroid belt, particularly into the near-Earth-object (NEO) region, and estimate the relative fluxes for different escape routes as a function of object size under the influence of the Yarkovsky semimajor-axis drift. Methods: We integrated the orbits of 78 355 known and 14 094 cloned main-belt objects and Cybele and Hilda asteroids (hereafter collectively called MBOs) for 100 Myr and recorded the characteristics of the escaping objects. The selected sample of MBOs with perihelion distance q > 1.3 au and semimajor axis a < 4.1 au is essentially complete, with an absolute magnitude limit ranging from HV < 15.9 in the inner belt (a < 2.5 au) to HV < 14.4 in the outer belt (2.5 au < a < 4.1 au). We modeled the semimajor-axis drift caused by the Yarkovsky force and assigned four different sizes (diameters of 0.1, 0.3, 1.0, and 3.0 km) and random spin obliquities (either 0 deg or 180 deg) for each test asteroid. Results: We find more than ten obvious escape routes from the asteroid belt to the NEO region, and they typically coincide with low-order mean-motion resonances with Jupiter and secular resonances. The locations of the escape routes are independent of the semimajor-axis drift rate and thus are also independent of the asteroid diameter. The locations of the escape routes are likewise unaffected when we added a model for Yarkovsky-O'Keefe-Radzievskii-Paddack (YORP) cycles coupled with secular evolution of the rotation pole as a result of the solar gravitational torque. A Yarkovsky-only model predicts a flux of asteroids entering the NEO region that is too high compared to the observationally constrained flux, and the discrepancy grows larger for smaller asteroids. A combined Yarkovsky and YORP model predicts a flux of small NEOs that is approximately a factor of 5 too low compared to an observationally constrained estimate. This suggests that the characteristic timescale of the YORP cycle is longer than our canonical YORP model predicts.

A critical review of the ESCAPE project for estimating long-term health effects of air pollution.

PubMed

Lipfert, Frederick W

2017-02-01

The European Study of Cohorts for Air Pollution Effects (ESCAPE) is a13-nation study of long-term health effects of air pollution based on subjects pooled from up to 22 cohorts that were intended for other purposes. Twenty-five papers have been published on associations of various health endpoints with long-term exposures to NOx, NO2, traffic indicators, PM10, PM2.5 and PM constituents including absorbance (elemental carbon). Seven additional ESCAPE papers found moderate correlations (R2=0.3-0.8) between measured air quality and estimates based on land-use regression that were used; personal exposures were not considered. I found no project summaries or comparisons across papers; here I conflate the 25 ESCAPE findings in the context of other recent European epidemiology studies. Because one ESCAPE cohort contributed about half of the subjects, I consider it and the other 18 cohorts separately to compare their contributions to the combined risk estimates. I emphasize PM2.5 and confirm the published hazard ratio of 1.14 (1.04-1.26) per 10μg/m3 for all-cause mortality. The ESCAPE papers found 16 statistically significant (p<0.05) risks among the125 pollutant-endpoint combinations; 4 each for PM2.5 and PM10, 1 for PM absorbance, 5 for NO2, and 2 for traffic. No PM constituent was consistently significant. No significant associations were reported for cardiovascular mortality; low birthrate was significant for all pollutants except PM absorbance. Based on associations with PM2.5, I find large differences between all-cause death estimates and the sum of specific-cause death estimates. Scatterplots of PM2.5 mortality risks by cause show no consistency across the 18 cohorts, ostensibly because of the relatively few subjects. Overall, I find the ESCAPE project inconclusive and I question whether the efforts required to estimate exposures for small cohorts were worthwhile. I suggest that detailed studies of the large cohort using historical exposures and additional cardiovascular risk factors might be productive. Copyright © 2016 Elsevier Ltd. All rights reserved.

Collisional Penrose process with spinning particles

NASA Astrophysics Data System (ADS)

Mukherjee, Sajal

2018-03-01

In this article, we have investigated collisional Penrose process (CPP) using spinning particles in a Kerr spacetime. Recent studies have shown that the collision between two spinning particles can produce a significantly high energy in the center of mass frame. Here, we explicitly compute the energy extraction and efficiency as measured by an observer at infinity. We consider the colliding particles as well as the escaping particles may contain spins. It has been shown that the energy extraction is larger than the non-spinning case and also their possibility to escape to infinity is wider than the geodesics.

Venusian hydrology: Steady state reconsidered

NASA Technical Reports Server (NTRS)

Grinspoon, David H.

1992-01-01

In 1987, Grinspoon proposed that the data on hydrogen abundance, isotopic composition, and escape rate were consistent with the hypothesis that water on Venus might be in steady state rather than monotonic decline since the dawn of time. This conclusion was partially based on a derived water lifetime against nonthermal escape of approximately 10(exp 8) yr. De Bergh et al., preferring the earlier Pioneer Venus value of 200 ppm water to the significantly lower value detected by Bezard et al., found H2O lifetimes of greater than 10(exp 9) yr. Donahue and Hodges derived H2O lifetimes of 0.4-5 x 10 (exp 9) yr. Both these analyses used estimates of H escape flux between 0.4 x 10(exp 7) and 1 x 10(exp 7) cm(exp -2)s(exp -1) from Rodriguez et al. Yet in more recent Monte Carlo modeling, Hodges and Tinsley found an escape flux due to charge exchange with hot H(+) of 2.8 x 10(exp 7) cm(exp -2)s(exp -1). McElroy et al. estimated an escape flux of 8 x 10(exp 6) cm(exp -2)s(exp -1) from collisions with hot O produced by dissociative recombination of O2(+). Brace et al. estimated an escape flux of 5 x 10(exp 6) cm(exp -2)s(exp -1) from ion escape from the ionotail of Venus. The combined estimated escape flux from all these processes is approximately 4 x 10(exp 7) cm(exp -2)s(exp -1). The most sophisticated analysis to date of near-IR radiation from Venus' nightside reveals a water mixing ratio of approximately 30 ppm, suggesting a lifetime against escape for water of less than 10(exp 8) yr. Large uncertainties remain in these quantities, yet the data point toward a steady state. Further evaluation of these uncertainties, and new evolutionary modeling incorporating estimates of the outgassing rate from post-Magellan estimates of the volcanic resurfacing rate are presented.

MIT January Operational Internship Experience

NASA Technical Reports Server (NTRS)

Bosanac, Natasha; DeVivero, Charlie; James, Jillian; Perez-Martinez, Carla; Pino, Wendy; Wang, Andrew; Willett, Ezekiel; Williams, Kwami

2010-01-01

This viewgraph presentation describes the MIT January Operational Internship Experience (JOIE) program. The topics include: 1) Landing and Recovery; 2) Transportation; 3) Shuttle Processing; 4) Constellation Processing; 5) External Tank; 6) Launch Pad; 7) Ground Operations; 8) Hypergolic Propellants; 9) Environmental; 10) Logistics; 11) Six Sigma; 12) Systems Engineering; and 13) Human Factors.

22 CFR 124.13 - Procurement by United States persons in foreign countries (offshore procurement).

Code of Federal Regulations, 2012 CFR

2012-04-01

... (build-to-print means producing an end-item (i.e., system, subsystem or component) from technical... of any information which discloses design methodology, engineering analysis, detailed process...

22 CFR 124.13 - Procurement by United States persons in foreign countries (offshore procurement).

Code of Federal Regulations, 2011 CFR

2011-04-01

... (build-to-print means producing an end-item (i.e., system, subsystem or component) from technical... of any information which discloses design methodology, engineering analysis, detailed process...

22 CFR 124.13 - Procurement by United States persons in foreign countries (offshore procurement).

Code of Federal Regulations, 2014 CFR

2014-04-01

... (build-to-print means producing an end-item (i.e., system, subsystem or component) from technical... of any information which discloses design methodology, engineering analysis, detailed process...

22 CFR 124.13 - Procurement by United States persons in foreign countries (offshore procurement).

Code of Federal Regulations, 2010 CFR

2010-04-01

... (build-to-print means producing an end-item (i.e., system, subsystem or component) from technical... of any information which discloses design methodology, engineering analysis, detailed process...

22 CFR 124.13 - Procurement by United States persons in foreign countries (offshore procurement).

Code of Federal Regulations, 2013 CFR

2013-04-01

... (build-to-print means producing an end-item (i.e., system, subsystem or component) from technical... of any information which discloses design methodology, engineering analysis, detailed process...

U.S.-Latin American Trade: Recent Trends

DTIC Science & Technology

2004-05-11

Export growth fell by 9.7% to Brazil, 37.8% to Venezuela , and 2.3% to the Dominican Republic. It rose, however, by 5.6% to Colombia, 3.8% to Chile...crisis in Venezuela , and the economic rebound in Argentina. Central America, as a region, escaped South America’s recent round of economic...from Mexico, 13.3% from Brazil, 7.1% from the Dominican Republic, 14.3% from Colombia, 13.2% from Venezuela , 9.7% from Costa Rica, and 7.1% from

Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors

PubMed Central

Smith, Tyrel T.; Moffett, Howell F.; Stephan, Sirkka B.; Opel, Cary F.; Dumigan, Amy G.; Jiang, Xiuyun; Pillarisetty, Venu G.; Pillai, Smitha P. S.; Wittrup, K. Dane; Stephan, Matthias T.

2017-01-01

Therapies using T cells that are programmed to express chimeric antigen receptors (CAR T cells) consistently produce positive results in patients with hematologic malignancies. However, CAR T cell treatments are less effective in solid tumors for several reasons. First, lymphocytes do not efficiently target CAR T cells; second, solid tumors create an immunosuppressive microenvironment that inactivates T cell responses; and third, solid cancers are typified by phenotypic diversity and thus include cells that do not express proteins targeted by the engineered receptors, enabling the formation of escape variants that elude CAR T cell targeting. Here, we have tested implantable biopolymer devices that deliver CAR T cells directly to the surfaces of solid tumors, thereby exposing them to high concentrations of immune cells for a substantial time period. In immunocompetent orthotopic mouse models of pancreatic cancer and melanoma, we found that CAR T cells can migrate from biopolymer scaffolds and eradicate tumors more effectively than does systemic delivery of the same cells. We have also demonstrated that codelivery of stimulator of IFN genes (STING) agonists stimulates immune responses to eliminate tumor cells that are not recognized by the adoptively transferred lymphocytes. Thus, these devices may improve the effectiveness of CAR T cell therapy in solid tumors and help protect against the emergence of escape variants. PMID:28436934

Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors.

PubMed

Smith, Tyrel T; Moffett, Howell F; Stephan, Sirkka B; Opel, Cary F; Dumigan, Amy G; Jiang, Xiuyun; Pillarisetty, Venu G; Pillai, Smitha P S; Wittrup, K Dane; Stephan, Matthias T

2017-06-01

Therapies using T cells that are programmed to express chimeric antigen receptors (CAR T cells) consistently produce positive results in patients with hematologic malignancies. However, CAR T cell treatments are less effective in solid tumors for several reasons. First, lymphocytes do not efficiently target CAR T cells; second, solid tumors create an immunosuppressive microenvironment that inactivates T cell responses; and third, solid cancers are typified by phenotypic diversity and thus include cells that do not express proteins targeted by the engineered receptors, enabling the formation of escape variants that elude CAR T cell targeting. Here, we have tested implantable biopolymer devices that deliver CAR T cells directly to the surfaces of solid tumors, thereby exposing them to high concentrations of immune cells for a substantial time period. In immunocompetent orthotopic mouse models of pancreatic cancer and melanoma, we found that CAR T cells can migrate from biopolymer scaffolds and eradicate tumors more effectively than does systemic delivery of the same cells. We have also demonstrated that codelivery of stimulator of IFN genes (STING) agonists stimulates immune responses to eliminate tumor cells that are not recognized by the adoptively transferred lymphocytes. Thus, these devices may improve the effectiveness of CAR T cell therapy in solid tumors and help protect against the emergence of escape variants.

Post-molting development of wind-elicited escape behavior in the cricket.

PubMed

Sato, Nodoka; Shidara, Hisashi; Ogawa, Hiroto

2017-11-01

Arthropods including insects grow through several developmental stages by molting. The abrupt changes in their body size and morphology accompanying the molting are responsible for the developmental changes in behavior. While in holometabolous insects, larval behaviors are transformed into adult-specific behaviors with drastic changes in nervous system during the pupal stage, hemimetabolous insects preserve most innate behaviors whole life long, which allow us to trace the maturation process of preserved behaviors after the changes in body. Wind-elicited escape behavior is one of these behaviors and mediated by cercal system, which is a mechanosensory organ equipped by all stages of nymph in orthopteran insects like crickets. However, the maturation process of the escape behavior after the molt is unclear. In this study, we examined time-series of changes in the wind-elicited escape behavior just after the imaginal molt in the cricket. The locomotor activities are developed over the elapsed time, and matured 24h after the molt. In contrast, a stimulus-angle dependency of moving direction was unchanged over time, meaning that the cercal sensory system detecting airflow direction was workable immediately after the molt, independent from the behavioral maturation. The post-molting development of the wind-elicited behavior was considered to result not simply from maturation of the exoskeleton or musculature because the escape response to heat-shock stimulus did not change after the molt. No effect of a temporal immobilization after the imaginal molt on the maturation of the wind-elicited behavior also implies that the maturation may be innately programmed without experience of locomotion. Copyright © 2017 Elsevier Ltd. All rights reserved.

Diffuse volcanic emissions of carbon dioxide from Vulcano Island, Italy.

PubMed

Baubron, J C; Allard, P; Toutain, J P

1990-03-01

RECENT investigations on Mount Etna (Sicily)(1-3) have revealed that volcanoes may release abundant carbon dioxide not only from their active craters, but also from their flanks, as diffuse soil emanations. Here we present analyses of soil gases and air in water wells on Vulcano Island which provide further evidence of such lateral degassing. Nearly pure carbon dioxide, enriched in helium and radon, escapes from the slopes of the Fossa active cone, adding a total output of 30 tonnes per day to the fumarolic crater discharge ( 180 tonnes CO(2) per day). This emanation has similar He/CO(2) and (13)C/(12)C ratios to those of the crater fumaroles (300%ndash;500 degrees C) and therefore a similar volcanic origin. Gases rich in carbon dioxide also escape at sea level along the isthmus between the Fossa and Vulcanello volcanic cones, but their depletion in both He and (13)C suggests a distinct source. Diffuse volcanic gas emanations, once their genetic link with central fumarole degassing has been demonstrated, can be used for continuous volcano monitoring, at safe distances from active craters. Such monitoring has been initiated at Vulcano, where soil and well emanations of nearly pure CO(2) themselves represent a threat to the local population.

Simulating dynamical features of escape panic

NASA Astrophysics Data System (ADS)

Helbing, Dirk; Farkas, Illés; Vicsek, Tamás

2000-09-01

One of the most disastrous forms of collective human behaviour is the kind of crowd stampede induced by panic, often leading to fatalities as people are crushed or trampled. Sometimes this behaviour is triggered in life-threatening situations such as fires in crowded buildings; at other times, stampedes can arise during the rush for seats or seemingly without cause. Although engineers are finding ways to alleviate the scale of such disasters, their frequency seems to be increasing with the number and size of mass events. But systematic studies of panic behaviour and quantitative theories capable of predicting such crowd dynamics are rare. Here we use a model of pedestrian behaviour to investigate the mechanisms of (and preconditions for) panic and jamming by uncoordinated motion in crowds. Our simulations suggest practical ways to prevent dangerous crowd pressures. Moreover, we find an optimal strategy for escape from a smoke-filled room, involving a mixture of individualistic behaviour and collective `herding' instinct.

Formation and role of exosomes in cancer.

PubMed

Brinton, Lindsey T; Sloane, Hillary S; Kester, Mark; Kelly, Kimberly A

2015-02-01

Exosomes offer new insight into cancer biology with both diagnostic and therapeutic implications. Because of their cell-to-cell communication, exosomes influence tumor progression, metastasis, and therapeutic efficacy. They can be isolated from blood and other bodily fluids to reveal disease processes occurring within the body, including cancerous growth. In addition to being a reservoir of cancer biomarkers, they can be re-engineered to reinstate tumor immunity. Tumor exosomes interact with various cells of the microenvironment to confer tumor-advantageous changes that are responsible for stromal activation, induction of the angiogenic switch, increased vascular permeability, and immune escape. Exosomes also contribute to metastasis by aiding in the epithelial-to-mesenchymal transition and formation of the pre-metastatic niche. Furthermore, exosomes protect tumor cells from the cytotoxic effects of chemotherapy drugs and transfer chemoresistance properties to nearby cells. Thus, exosomes are essential to many lethal elements of cancer and it is important to understand their biogenesis and role in cancer.

Role of the ionosphere for the atmospheric evolution of planets.

PubMed

Yamauchi, Masatoshi; Wahlund, Jan-Erik

2007-10-01

We have synthesized current understanding, mainly observations, with regard to ion escape mechanisms to space from the ionosphere and exosphere of Titan and Earth-type planets, with the intent to provide an improved input for models of atmospheric evolution on early Earth and Earth-type planets and exoplanets. We focus on the role of the ionosphere and its non-linear response to solar parameters, all of which have been underestimated in current models of ancient atmospheric escape (4 billion years ago). Factors that have been overlooked include the following: (1) Much larger variation of O(+) outflow than H(+) outflow from the terrestrial ionosphere, depending on solar and geomagnetic activities (an important consideration when attempting to determine the oxidized state of the atmosphere of early Earth); (2) magnetization of the ionopause, which keeps ionospheric ions from escaping and controls many other escape processes; (3) extra ionization by, for example, the critical ionization velocity mechanism, which expands the ionosphere to greater altitudes than current models predict; and (4) the large escape of cold ions from the dense, expanded ionosphere of Titan. Here we offer, as a guideline for quantitative simulations, a qualitative diagnosis of increases or decreases of non-thermal escape related to the ionosphere for magnetized and unmagnetized planets in response to changes in solar parameters (i.e., solar EUV/FUV flux, solar wind dynamic pressure, and interplanetary magnetic field).

Near-horizon Structure of Escape Zones of Electrically Charged Particles around Weakly Magnetized Rotating Black Hole

NASA Astrophysics Data System (ADS)

Kopáček, Ondřej; Karas, Vladimír

2018-01-01

An interplay of magnetic fields and gravitation drives accretion and outflows near black holes. However, a specific mechanism is still a matter of debate; it is very likely that different processes dominate under various conditions. In particular, for the acceleration of particles and their collimation in jets, an ordered component of the magnetic field seems to be essential. Here we discuss the role of large-scale magnetic fields in transporting the charged particles and dust grains from the bound orbits in the equatorial plane of a rotating (Kerr) black hole and the resulting acceleration along trajectories escaping the system in a direction parallel to the symmetry axis (perpendicular to the accretion disk). We consider a specific scenario of destabilization of circular geodesics of initially neutral matter by charging (e.g., due to photoionization). Some particles may be set on escaping trajectories and attain relativistic velocity. The case of charged particles differs from charged dust grains by their charge-to-mass ratio, but the acceleration mechanism operates in a similar manner. It appears that the chaotic dynamics controls the outflow and supports the formation of near-horizon escape zones. We employ the technique of recurrence plots to characterize the onset of chaos in the outflowing medium. We investigate the system numerically and construct the basin-boundary plots, which show the location and the extent of the escape zones. The effects of black hole spin and magnetic field strength on the formation and location of escape zones are discussed, and the maximal escape velocity is computed.

Generalized Jeans' Escape of Pick-Up Ions in Quasi-Linear Relaxation

NASA Technical Reports Server (NTRS)

Moore, T. E.; Khazanov, G. V.

2011-01-01

Jeans escape is a well-validated formulation of upper atmospheric escape that we have generalized to estimate plasma escape from ionospheres. It involves the computation of the parts of particle velocity space that are unbound by the gravitational potential at the exobase, followed by a calculation of the flux carried by such unbound particles as they escape from the potential well. To generalize this approach for ions, we superposed an electrostatic ambipolar potential and a centrifugal potential, for motions across and along a divergent magnetic field. We then considered how the presence of superthermal electrons, produced by precipitating auroral primary electrons, controls the ambipolar potential. We also showed that the centrifugal potential plays a small role in controlling the mass escape flux from the terrestrial ionosphere. We then applied the transverse ion velocity distribution produced when ions, picked up by supersonic (i.e., auroral) ionospheric convection, relax via quasi-linear diffusion, as estimated for cometary comas [1]. The results provide a theoretical basis for observed ion escape response to electromagnetic and kinetic energy sources. They also suggest that super-sonic but sub-Alfvenic flow, with ion pick-up, is a unique and important regime of ion-neutral coupling, in which plasma wave-particle interactions are driven by ion-neutral collisions at densities for which the collision frequency falls near or below the gyro-frequency. As another possible illustration of this process, the heliopause ribbon discovered by the IBEX mission involves interactions between the solar wind ions and the interstellar neutral gas, in a regime that may be analogous [2].

Space Weather at Mars: 3-D studies using one-way coupling between the Multi-fluid MHD, M-GITM and M-AMPS models

NASA Astrophysics Data System (ADS)

Dong, Chuanfei

This dissertation presents numerical simulation results of the solar wind interaction with the Martian upper atmosphere by using three comprehensive 3-D models: the Mars Global Ionosphere Thermosphere Model (M-GITM), the Mars exosphere Monte Carlo model Adaptive Mesh Particle Simulator (M-AMPS), and the BATS-R-US Mars multi-fluid MHD (MF-MHD) model. The coupled framework has the potential to provide improved predictions for ion escape rates for comparison with future data to be returned by the MAVEN mission (2014-2016) and thereby improve our understanding of present day escape processes. Estimates of ion escape rates over Mars history must start from properly validated models that can be extrapolated into the past. This thesis aims to build a model library for the NASA Mars Atmosphere and Volatile EvolutioN (MAVEN) mission, which will thus enhance the science return from the MAVEN mission. In this thesis, we aim to address the following four main scientific questions by adopting the one-way coupled framework developed here: (1) What are the Martian ion escape rates at the current epoch and ancient times? (2) What controls the ion escape processes at the current epoch? How are the ion escape variations connected to the solar cycle, crustal field orientation and seasonal variations? (3) How do the variable 3-D cold neutral thermosphere and hot oxygen corona affect the solar wind-Mars interaction? (4) How does the Martian atmosphere respond to extreme variations (e.g., ICMEs) in the solar wind and its interplanetary environment? These questions are closely related to the primary scientific goals of NASA's MAVEN mission and European Space Agency's Mars Express (MEX) mission. We reasonably answer all these four questions at the end of this thesis by employing the one-way coupled framework and comparing the simulation results with both MEX and MAVEN observational data.

Generating favorable growth factor and protease release profiles to enable extracellular matrix accumulation within an in vitro tissue engineering environment.

PubMed

Zhang, Xiaoqing; Battiston, Kyle G; Labow, Rosalind S; Simmons, Craig A; Santerre, J Paul

2017-05-01

Tissue engineering (particularly for the case of load-bearing cardiovascular and connective tissues) requires the ability to promote the production and accumulation of extracellular matrix (ECM) components (e.g., collagen, glycosaminoglycan and elastin). Although different approaches have been attempted in order to enhance ECM accumulation in tissue engineered constructs, studies of underlying signalling mechanisms that influence ECM deposition and degradation during tissue remodelling and regeneration in multi-cellular culture systems have been limited. The current study investigated vascular smooth muscle cell (VSMC)-monocyte co-culture systems using different VSMC:monocyte ratios, within a degradable polyurethane scaffold, to assess their influence on ECM generation and degradation processes, and to elucidate relevant signalling molecules involved in this in vitro vascular tissue engineering system. It was found that a desired release profile of growth factors (e.g. insulin growth factor-1 (IGF-1)) and hydrolytic proteases (e.g. matrix-metalloproteinases 2, 9, 13 and 14 (MMP2, MMP9, MMP13 and MMP14)), could be achieved in co-culture systems, yielding an accumulation of ECM (specifically for 2:1 and 4:1 VSMC:monocyte culture systems). This study has significant implications for the tissue engineering field (including vascular tissue engineering), not only because it identified important cytokines and proteases that control ECM accumulation/degradation within synthetic tissue engineering scaffolds, but also because the established culture systems could be applied to improve the development of different types of tissue constructs. Sufficient extracellular matrix accumulation within cardiovascular and connective tissue engineered constructs is a prerequisite for their appropriate function in vivo. This study established co-culture systems with tissue specific cells (vascular smooth muscle cells (VSMCs)) and defined ratios of immune cells (monocytes) to investigate extracellular matrix (ECM) generation and degradation processes, revealing important mechanisms underlying ECM turnover during vascular tissue regeneration/remodelling. A specific growth factor (IGF-1), as well as hydrolytic proteases (e.g. MMP2, MMP9, MMP13 and MMP14), were identified as playing important roles in these processes. ECM accumulation was found to be dependent on achieving a desired release profile of these ECM-promoting and ECM-degrading factors within the multi-cellular microenvironment. The findings enhance our understanding of ECM deposition and degradation during in vitro tissue engineering and would be applicable to the repair or regeneration of a variety of tissues. Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Advanced Concept

NASA Image and Video Library

2003-12-01

This photo gives an overhead look at an RS-88 development rocket engine being test fired at NASA's Marshall Space Flight Center in Huntsville, Alabama, in support of the Pad Abort Demonstration (PAD) test flights for NASA's Orbital Space Plane (OSP). The tests could be instrumental in developing the first crew launch escape system in almost 30 years. Paving the way for a series of integrated PAD test flights, the engine tests support development of a system that could pull a crew safely away from danger during liftoff. A series of 16 hot fire tests of a 50,000-pound thrust RS-88 rocket engine were conducted, resulting in a total of 55 seconds of successful engine operation. The engine is being developed by the Rocketdyne Propulsion and Power unit of the Boeing Company. Integrated launch abort demonstration tests in 2005 will use four RS-88 engines to separate a test vehicle from a test platform, simulating pulling a crewed vehicle away from an aborted launch. Four 156-foot parachutes will deploy and carry the vehicle to landing. Lockheed Martin is building the vehicles for the PAD tests. Seven integrated tests are plarned for 2005 and 2006.

Advanced Concept

NASA Image and Video Library

2003-12-01

In this photo, an RS-88 development rocket engine is being test fired at NASA's Marshall Space Flight Center in Huntsville, Alabama, in support of the Pad Abort Demonstration (PAD) test flights for NASA's Orbital Space Plane (OSP). The tests could be instrumental in developing the first crew launch escape system in almost 30 years. Paving the way for a series of integrated PAD test flights, the engine tests support development of a system that could pull a crew safely away from danger during liftoff. A series of 16 hot fire tests of a 50,000-pound thrust RS-88 rocket engine were conducted, resulting in a total of 55 seconds of successful engine operation. The engine is being developed by the Rocketdyne Propulsion and Power unit of the Boeing Company. Integrated launch abort demonstration tests in 2005 will use four RS-88 engines to separate a test vehicle from a test platform, simulating pulling a crewed vehicle away from an aborted launch. Four 156-foot parachutes will deploy and carry the vehicle to landing. Lockheed Martin is building the vehicles for the PAD tests. Seven integrated tests are plarned for 2005 and 2006.

Martian Atmospheric and Ionospheric plasma Escape

NASA Astrophysics Data System (ADS)

Lundin, Rickard

2016-04-01

Solar forcing is responsible for the heating, ionization, photochemistry, and erosion processes in the upper atmosphere throughout the lifetime of the terrestrial planets. Of the four terrestrial planets, the Earth is the only one with a fully developed biosphere, while our kin Venus and Mars have evolved into arid inhabitable planets. As for Mars, there are ample evidences for an early Noachian, water rich period on Mars. The question is, what made Mars evolve so differently compared to the Earth? Various hydrosphere and atmospheric evolution scenarios for Mars have been forwarded based on surface morphology, chemical composition, simulations, semi-empiric (in-situ data) models, and the long-term evolution of the Sun. Progress has been made, but the case is still open regarding the changes that led to the present arid surface and tenuous atmosphere at Mars. This presentation addresses the long-term variability of the Sun, the solar forcing impact on the Martian atmosphere, and its interaction with the space environment - an electromagnetic wave and particle interaction with the upper atmosphere that has implications for its photochemistry, composition, and energization that governs thermal and non-thermal escape. Non-thermal escape implies an electromagnetic upward energization of planetary ions and molecules to velocities above escape velocity, a process governed by a combination of solar EUV radiation (ionization), and energy and momentum transfer by the solar wind. The ion escape issue dates back to the early Soviet and US-missions to Mars, but the first more accurate estimates of escape rates came with the Phobos-2 mission in 1989. Better-quality ion composition measurement results of atmospheric/ionospheric ion escape from Mars, obtained from ESA Mars Express (MEX) instruments, have improved our understanding of the ion escape mechanism. With the NASA MAVEN spacecraft orbiting Mars since Sept. 2014, dual in-situ measurement with plasma instruments are now carried out in the Martian planetary realm. Of particular interest from a planetary atmospheric escape point of view is the long-term implications of solar forcing. From ASPERA-data on MEX it has been possible to cover the transition from cycle 23 up to the cycle 24 maximum, data displaying clear solar cycle dependence. The planetary ion escape rate increased from solar minimum to solar maximum by a factor of 10. From a regression analysis using ion escape fluxes and solar forcing proxies, a "back-casting" tool is developed [1], enabling determination of the planetary ion escape back in time based on long-term solar forcing proxies (F10.7, sunspot number). The tool may be applied to other long-term solar proxies, such as the radiogenic isotopes in the Earth's atmosphere, 10Be and 14C. The cosmic-ray production of these long-lifetime (>10000 year) isotopes is modulated by the solar-heliospheric magnetic flux, i.e. an indirect measure of solar magnetic activity. Beyond that there is so far only one additional rough "back-casting" tool, the "Sun-in-time", a method whereby the age of, EUV/UV radiation, and mass-loss of other sun-like stars are determined [2, 3]. [1] Lundin et al., Geophys. Res. Lett., 40, 23, pp. 6028-6032, 2013. [2] Wood et al., ApJ, 574:412-425, 2002. [3] Ribas et al., ApJ., 622:680-694, 2005

Trends in spawning populations of Pacific anadromous salmonids

USGS Publications Warehouse

Konkel, G.W.; McIntyre, J.D.

1987-01-01

Annual escapement records for 1968-1984 for five species of Pacific salmon-chinook (Oncorhynchus tshawytscha), coho (O. kisutch), sockeye (O. nerka), pink (O. gorbuscha), and chum (O. keta)—and steelhead (Salmo gairdneri) were obtained from published and unpublished sources and organized in a computer database. More than 25,500 escapement records were obtained for more than 1,100 locations throughout Alaska, Washington, Idaho, Oregon, and California. Escapement trends for naturally reproducing populations for which data were available for at least 7 years from 1968 to 1984 and at least 4 years from 1975 to 1984 were analyzed by linear regression. Significant trends were observed in about 30% of the 886 populations examined. Trends were summarized by species for three geographic regions in Alaska and four in the Pacific Northwest (including California). For chinook, sockeye, and pink salmon, trends were predominantly increasing in the Alaska regions and either lacking or predominantly decreasing in most of the Pacific Northwest regions; for coho and chum salmon, trends were predominantly decreasing in one or more Alaska regions as well as in most of the Pacific Northwest regions. For steelhead, too few populations were examined to enable us to characterize trends throughout their range. Among the 657 salmonid populations excluded from the trend analysis because the data sets were incomplete, 13 (of which 2 were in Alaska) declined to zero during the period of analysis. For coho, sockeye, pink, and chum salmon and steelhead, major data gaps were revealed by a comparison of the geographic distribution of escapement records with the spawning distribution of the species. For chinook salmon, escapement records were more geographically representative of the spawning distribution.

XUV-Exposed, Non-Hydrostatic Hydrogen-Rich Upper Atmospheres of Terrestrial Planets. Part I: Atmospheric Expansion and Thermal Escape

PubMed Central

Lammer, Helmut; Odert, Petra; Kulikov, Yuri N.; Kislyakova, Kristina G.; Khodachenko, Maxim L.; Güdel, Manuel; Hanslmeier, Arnold; Biernat, Helfried

2013-01-01

Abstract The recently discovered low-density “super-Earths” Kepler-11b, Kepler-11f, Kepler-11d, Kepler-11e, and planets such as GJ 1214b represent the most likely known planets that are surrounded by dense H/He envelopes or contain deep H2O oceans also surrounded by dense hydrogen envelopes. Although these super-Earths are orbiting relatively close to their host stars, they have not lost their captured nebula-based hydrogen-rich or degassed volatile-rich steam protoatmospheres. Thus, it is interesting to estimate the maximum possible amount of atmospheric hydrogen loss from a terrestrial planet orbiting within the habitable zone of late main sequence host stars. For studying the thermosphere structure and escape, we apply a 1-D hydrodynamic upper atmosphere model that solves the equations of mass, momentum, and energy conservation for a planet with the mass and size of Earth and for a super-Earth with a size of 2 REarth and a mass of 10 MEarth. We calculate volume heating rates by the stellar soft X-ray and extreme ultraviolet radiation (XUV) and expansion of the upper atmosphere, its temperature, density, and velocity structure and related thermal escape rates during the planet's lifetime. Moreover, we investigate under which conditions both planets enter the blow-off escape regime and may therefore experience loss rates that are close to the energy-limited escape. Finally, we discuss the results in the context of atmospheric evolution and implications for habitability of terrestrial planets in general. Key Words: Stellar activity—Low-mass stars—Early atmospheres—Earth-like exoplanets—Energetic neutral atoms—Ion escape—Habitability. Astrobiology 13, 1011–1029. PMID:24251443

Generation of ethylene tracer by noncatalytic pyrolysis of natural gas at elevated pressure

USGS Publications Warehouse

Lu, Y.; Chen, S.; Rostam-Abadi, M.; Ruch, R.; Coleman, D.; Benson, L.J.

2005-01-01

There is a critical need within the pipeline gas industry for an inexpensive and reliable technology to generate an identification tag or tracer that can be added to pipeline gas to identify gas that may escape and improve the deliverability and management of gas in underground storage fields. Ethylene is an ideal tracer, because it does not exist naturally in the pipeline gas, and because its physical properties are similar to the pipeline gas components. A pyrolysis process, known as the Tragen process, has been developed to continuously convert the ???2%-4% ethane component present in pipeline gas into ethylene at common pipeline pressures of 800 psi. In our studies of the Tragen process, pyrolysis without steam addition achieved a maximum ethylene yield of 28%-35% at a temperature range of 700-775 ??C, corresponding to an ethylene concentration of 4600-5800 ppm in the product gas. Coke deposition was determined to occur at a significant rate in the pyrolysis reactor without steam addition. The ?? 13C isotopic analysis of gas components showed a ?? 13C value of ethylene similar to ethane in the pipeline gas, indicating that most of the ethylene was generated from decomposition of the ethane in the raw gas. However, ?? 13C isotopic analysis of the deposited coke showed that coke was primarily produced from methane, rather than from ethane or other heavier hydrocarbons. No coke deposition was observed with the addition of steam at concentrations of > 20% by volume. The dilution with steam also improved the ethylene yield. ?? 2005 American Chemical Society.

Efficient Processing of the Immunodominant, HLA-A*0201-Restricted Human Immunodeficiency Virus Type 1 Cytotoxic T-Lymphocyte Epitope despite Multiple Variations in the Epitope Flanking Sequences

PubMed Central

Brander, Christian; Yang, Otto O.; Jones, Norman G.; Lee, Yun; Goulder, Philip; Johnson, R. Paul; Trocha, Alicja; Colbert, David; Hay, Christine; Buchbinder, Susan; Bergmann, Cornelia C.; Zweerink, Hans J.; Wolinsky, Steven; Blattner, William A.; Kalams, Spyros A.; Walker, Bruce D.

1999-01-01

Immune escape from cytotoxic T-lymphocyte (CTL) responses has been shown to occur not only by changes within the targeted epitope but also by changes in the flanking sequences which interfere with the processing of the immunogenic peptide. However, the frequency of such an escape mechanism has not been determined. To investigate whether naturally occurring variations in the flanking sequences of an immunodominant human immunodeficiency virus type 1 (HIV-1) Gag CTL epitope prevent antigen processing, cells infected with HIV-1 or vaccinia virus constructs encoding different patient-derived Gag sequences were tested for recognition by HLA-A*0201-restricted, p17-specific CTL. We found that the immunodominant p17 epitope (SL9) and its variants were efficiently processed from minigene expressing vectors and from six HIV-1 Gag variants expressed by recombinant vaccinia virus constructs. Furthermore, SL9-specific CTL clones derived from multiple donors efficiently inhibited virus replication when added to HLA-A*0201-bearing cells infected with primary or laboratory-adapted strains of virus, despite the variability in the SL9 flanking sequences. These data suggest that escape from this immunodominant CTL response is not frequently accomplished by changes in the epitope flanking sequences. PMID:10559335

TIGRESS: TRIUMF-ISAC gamma-ray escape-suppressed spectrometer

NASA Astrophysics Data System (ADS)

Svensson, C. E.; Amaudruz, P.; Andreoiu, C.; Andreyev, A.; Austin, R. A. E.; Ball, G. C.; Bandyopadhyay, D.; Boston, A. J.; Chakrawarthy, R. S.; Chen, A. A.; Churchman, R.; Drake, T. E.; Finlay, P.; Garrett, P. E.; Grinyer, G. F.; Hackman, G.; Hyland, B.; Jones, B.; Kanungo, R.; Maharaj, R.; Martin, J. P.; Morris, D.; Morton, A. C.; Pearson, C. J.; Phillips, A. A.; Ressler, J. J.; Roy, R.; Sarazin, F.; Schumaker, M. A.; Scraggs, H. C.; Smith, M. B.; Starinsky, N.; Valiente-Dobón, J. J.; Waddington, J. C.; Watters, L. M.

2005-10-01

The TRIUMF-ISAC gamma-ray escape-suppressed spectrometer (TIGRESS) is a new γ-ray detector array being developed for use at TRIUMF's Isotope Separator and Accelerator (ISAC) radioactive ion beam facility. TIGRESS will comprise 12 32-fold segmented clover-type HPGe detectors coupled with 20-fold segmented modular Compton suppression shields and custom digital signal processing electronics. This paper provides an overview of the TIGRESS project and progress in its development to date.

Nano-biomimetic carriers are implicated in mechanistic evaluation of intracellular gene delivery

NASA Astrophysics Data System (ADS)

Alipour, Mohsen; Hosseinkhani, Saman; Sheikhnejad, Reza; Cheraghi, Roya

2017-01-01

Several tissue specific non-viral carriers have been developed for gene delivery purposes. However, the inability to escape endosomes, undermines the efficacy of these carriers. Researchers inspired by HIV and influenza virus, have randomly used Gp41 and H5WYG fusogenic peptides in several gene delivery systems without any rational preference. Here for the first time, we have genetically engineered two Nano-biomimetic carriers composed of either HWYG (HNH) or Gp41 (GNH) that precisely provide identical conditions for the study and evaluation of these fusogenic peptides. The luciferase assay demonstrated a two-fold higher transfection efficiency of HNH compared to GNH. These nanocarriers also displayed equivalent properties in terms of DNA binding ability and DNA protection against serum nucleases and formed similar nanoparticles in terms of surface charge and size. Interestingly, hemolysis and cellular analysis demonstrated both of nanoparticles internalized into cells in similar rate and escaped from endosome with different efficiency. Furthermore, the structural analysis revealed the mechanisms responsible for the superior endosomal escaping capability of H5WYG. In conclusion, this study describes the rationale for using H5WYG peptide to deliver nucleic acids and suggests that using nano-biomimetic carriers to screen different endosomal release peptides, improves gene delivery significantly.

Super Mario's prison break —A proposal of object-intelligent-feedback-based classical Zeno and anti-Zeno effects

NASA Astrophysics Data System (ADS)

Gu, Shi-Jian

2009-10-01

Super Mario is imprisoned by a demon in a finite potential well on his way to save Princess Peach. He can escape from the well with the help of a flight of magic stairs floating in the space. However, the hateful demon may occasionally check his status. At that time, he has to make a judgement of either jumping to the inside ground immediately in order to avoid the discovery of his escape intention, or speeding up his escape process. Therefore, if the demon checks him too frequently such that there is no probability for him to reach the top of the barrier, he will be always inside the well, then a classical Zeno effect occurs. On the other hand, if the time interval between two subsequent checks is large enough such that he has a higher probability of being beyond the demon's controllable range already, then the demon's check actually speeds up his escape and a classical anti-Zeno effect takes place.

MOLECULAR-KINETIC SIMULATIONS OF ESCAPE FROM THE EX-PLANET AND EXOPLANETS: CRITERION FOR TRANSONIC FLOW

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, Robert E.; Volkov, Alexey N.; Erwin, Justin T.

The equations of gas dynamics are extensively used to describe atmospheric loss from solar system bodies and exoplanets even though the boundary conditions at infinity are not uniquely defined. Using molecular-kinetic simulations that correctly treat the transition from the continuum to the rarefied region, we confirm that the energy-limited escape approximation is valid when adiabatic expansion is the dominant cooling process. However, this does not imply that the outflow goes sonic. Rather large escape rates and concomitant adiabatic cooling can produce atmospheres with subsonic flow that are highly extended. Since this affects the heating rate of the upper atmosphere andmore » the interaction with external fields and plasmas, we give a criterion for estimating when the outflow goes transonic in the continuum region. This is applied to early terrestrial atmospheres, exoplanet atmospheres, and the atmosphere of the ex-planet, Pluto, all of which have large escape rates.« less

Selection of antigenically advanced variants of seasonal influenza viruses.

PubMed

Li, Chengjun; Hatta, Masato; Burke, David F; Ping, Jihui; Zhang, Ying; Ozawa, Makoto; Taft, Andrew S; Das, Subash C; Hanson, Anthony P; Song, Jiasheng; Imai, Masaki; Wilker, Peter R; Watanabe, Tokiko; Watanabe, Shinji; Ito, Mutsumi; Iwatsuki-Horimoto, Kiyoko; Russell, Colin A; James, Sarah L; Skepner, Eugene; Maher, Eileen A; Neumann, Gabriele; Klimov, Alexander I; Kelso, Anne; McCauley, John; Wang, Dayan; Shu, Yuelong; Odagiri, Takato; Tashiro, Masato; Xu, Xiyan; Wentworth, David E; Katz, Jacqueline M; Cox, Nancy J; Smith, Derek J; Kawaoka, Yoshihiro

2016-05-23

Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. We also selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014-2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature.

Compaction and gas loss in welded pyroclastic deposits as revealed by porosity, permeability, and electrical conductivity measurements of the Shevlin Park Tuff

USGS Publications Warehouse

Wright, Heather M.; Cashman, Katharine V.

2014-01-01

Pyroclastic flows produced by large volcanic eruptions commonly densify after emplacement. Processes of gas escape, compaction, and welding in pyroclastic-flow deposits are controlled by the physical and thermal properties of constituent material. Through measurements of matrix porosity, permeability, and electrical conductivity, we provide a framework for understanding the evolution of pore structure during these processes. Using data from the Shevlin Park Tuff in central Oregon, United States, and from the literature, we find that over a porosity range of 0%–70%, matrix permeability varies by almost 10 orders of magnitude (from 10–20 to 10–11 m2), with over three orders of magnitude variation at any given porosity. Part of the variation at a given porosity is due to permeability anisotropy, where oriented core samples indicate higher permeabilities parallel to foliation (horizontally) than perpendicular to foliation (vertically). This suggests that pore space is flattened during compaction, creating anisotropic crack-like networks, a geometry that is supported by electrical conductivity measurements. We find that the power law equation: k1 = 1.3 × 10–21 × ϕ5.2 provides the best approximation of dominant horizontal gas loss, where k1 = permeability, and ϕ = porosity. Application of Kozeny-Carman fluid-flow approximations suggests that permeability in the Shevlin Park Tuff is controlled by crack- or disk-like pore apertures with minimum widths of 0.3 and 7.5 μm. We find that matrix permeability limits compaction over short times, but deformation is then controlled by competition among cooling, compaction, water resorption, and permeable gas escape. These competing processes control the potential for development of overpressure (and secondary explosions) and the degree of welding in the deposit, processes that are applicable to viscous densification of volcanic deposits in general. Further, the general relationships among porosity, permeability, and pore geometry are relevant for flow of any fluid through an ignimbritic host.

The cortical mouse: a piece of forgotten history in noninvasive brain–computer interfaces.

PubMed

Principe, Jose C

2013-07-01

Early research on brain-computer interfaces (BCIs) was fueled by the study of event-related potentials (ERPs) by Farwell and Donchin, who are rightly credited for laying important groundwork for the BCI field. However, many other researchers have made substantial contributions that have escaped the radar screen of the current BCI community. For example, in the late 1980s, I worked with a brilliant multidisciplinary research group in electrical engineering at the University of Florida, Gainesville, headed by Dr. Donald Childers. Childers should be well known to long-time members of the IEEE Engineering in Medicine and Biology Society since he was the editor-in-chief of IEEE Transactions on Biomedical Engineering in the 1970s and the recipient of one of the most prestigious society awards, the William J. Morlock Award, in 1973.

Predictive Engineering Tools for Injection-Molded Long-Carbon-Fiber Thermoplastic Composites - FY13 Fourth Quarterly Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Ba Nghiep; Simmons, Kevin L.

2013-12-02

This quarterly report summarizes the status of the project planning to obtain all the approvals required for a Cooperative Research and Development Agreement (CRADA) with Autodesk, Inc., Toyota Motor Engineering and Manufacturing North America (Toyota), and Magna Exterior and Interiors Corporation (Magna). The final CRADA documents processed by PNNL’s Legal Services were submitted to all the parties for signatures.

Standard CMMI Appraisal Method for Process Improvement (SCAMPI) A, Version 1.3: Method Definition Document

DTIC Science & Technology

2011-03-01

performance of Federal Government Contract Number FA8721-05- C -0003 with Carnegie Mellon University for the operation of the Software Engineering... C Roles and Responsibilities 195 Appendix D Reporting Requirements and Options 201 Appendix E Managed Discovery 203 Appendix F Scoping and...Upgrade Team (SUT) • Mary Busby , Lockheed Martin • Palma Buttles-Valdez, Software Engineering Institute • Paul Byrnes, Integrated System Diagnostics

Peer Review of Launch Environments

NASA Technical Reports Server (NTRS)

Wilson, Timmy R.

2011-01-01

Catastrophic failures of launch vehicles during launch and ascent are currently modeled using equivalent trinitrotoluene (TNT) estimates. This approach tends to over-predict the blast effect with subsequent impact to launch vehicle and crew escape requirements. Bangham Engineering, located in Huntsville, Alabama, assembled a less-conservative model based on historical failure and test data coupled with physical models and estimates. This white paper summarizes NESC's peer review of the Bangham analytical work completed to date.

The Baltimore Engineers and the Chesapeake Bay, 1961-1987

DTIC Science & Technology

1988-01-01

supply and drought management study that will identify those measures required to optimize the use of exist- ing water supplies in the Bay drainage ... drainage area of the Chesapeake, the Susquehanna accounts for 43% and the Potomac for 22% of this land area. The total average inflow of fresh water to...right) water supply and, tn some areas, abatement of acid mine drainage . not allowed the Susquehanna to escape from serious water supply

An Assessment of the U.S. Army Tank Automotive Research, Development and Engineering Center’s Utilization of the Processes, and Availability of Tools and Physical Environments that Promote Innovation

DTIC Science & Technology

2014-04-02

workspaces Proximity H13 : TARDEC associates do not sit near other associates with different functional expertise Objective The objective of...Physical Environment Collaborative Workspace H12: TARDEC does not have available innovation best practice collaborative workspaces Proximity H13 ...does not have available innovation best practice collaborative workspaces Proximity H13 : TARDEC associates do not sit near other associates with

Effects of evacuation assistant’s leading behavior on the evacuation efficiency: Information transmission approach

NASA Astrophysics Data System (ADS)

Wang, Xiao-Lu; Guo, Wei; Zheng, Xiao-Ping

2015-07-01

Evacuation assistants are expected to spread the escape route information and lead evacuees toward the exit as quickly as possible. Their leading behavior influences the evacuees’ movement directly, which is confirmed to be a decisive factor of the evacuation efficiency. The transmission process of escape information and its function on the evacuees’ movement are accurately presented by the proposed extended dynamic communication field model. For evacuation assistants and evacuees, their sensitivity parameter of static floor field (SFF), , and , are fully discussed. The simulation results indicate that the appropriate is associated with the maximum of evacuees. The optimal combinations of and were found to reach the highest evacuation efficiency. There also exists an optimal value for evacuation assistants’ information transmission radius. Project supported by the National Basic Research Program of China (Grant No. 2011CB706900), the National Natural Science Foundation of China (Grant Nos. 71225007 and 71203006), the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (Grant No. 2012BAK13B06), the Humanities and Social Sciences Project of the Ministry of Education of China (Grant Nos. 10YJA630221 and 12YJCZH023), and the Beijing Philosophy and Social Sciences Planning Project of the Twelfth Five-Year Plan, China (Grant Nos. 12JGC090 and 12JGC098).

Visible sub-band gap photoelectron emission from nitrogen doped and undoped polycrystalline diamond films

NASA Astrophysics Data System (ADS)

Elfimchev, S.; Chandran, M.; Akhvlediani, R.; Hoffman, A.

2017-07-01

In this study the origin of visible sub-band gap photoelectron emission (PEE) from polycrystalline diamond films is investigated. The PEE yields as a function of temperature were studied in the wavelengths range of 360-520 nm. Based on the comparison of electron emission yields from diamond films deposited on silicon and molybdenum substrates, with different thicknesses and nitrogen doping levels, we suggested that photoelectrons are generated from nitrogen related centers in diamond. Our results show that diamond film thickness and substrate material have no significant influence on the PEE yield. We found that nanocrystalline diamond films have low electron emission yields, compared to microcrystalline diamond, due to the presence of high amount of defects in the former, which trap excited electrons before escaping into the vacuum. However, the low PEE yield of nanocrystalline diamond films was found to increase with temperature. The phenomenon was explained by the trap assisted photon enhanced thermionic emission (ta-PETE) model. According to the ta-PETE model, photoelectrons are trapped by shallow traps, followed by thermal excitation at elevated temperatures and escape into the vacuum. Activation energies of trap levels were estimated for undoped nanocrystalline, undoped microcrystalline and N-doped diamond films using the Richardson-Dushman equation, which gives 0.13, 0.39 and 0.04 eV, respectively. Such low activation energy of trap levels makes the ta-PETE process very effective at elevated temperatures.

NASA’s MAVEN Mission Observes Ups and Downs of Water Escape from Mars

NASA Image and Video Library

2017-12-08

After investigating the upper atmosphere of the Red Planet for a full Martian year, NASA’s MAVEN mission has determined that the escaping water does not always go gently into space. Sophisticated measurements made by a suite of instruments on the Mars Atmosphere and Volatile Evolution, or MAVEN, spacecraft revealed the ups and downs of hydrogen escape – and therefore water loss. The escape rate peaked when Mars was at its closest point to the sun and dropped off when the planet was farthest from the sun. The rate of loss varied dramatically overall, with 10 times more hydrogen escaping at the maximum. “MAVEN is giving us unprecedented detail about hydrogen escape from the upper atmosphere of Mars, and this is crucial for helping us figure out the total amount of water lost over billions of years,” said Ali Rahmati, a MAVEN team member at the University of California at Berkeley who analyzed data from two of the spacecraft’s instruments. Hydrogen in Mars’ upper atmosphere comes from water vapor in the lower atmosphere. An atmospheric water molecule can be broken apart by sunlight, releasing the two hydrogen atoms from the oxygen atom that they had been bound to. Several processes at work in Mars’ upper atmosphere may then act on the hydrogen, leading to its escape. Read more: go.nasa.gov/2dAgAV4 NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Comparative pick-up ion distributions at Mars and Venus: Consequences for atmospheric deposition and escape

NASA Astrophysics Data System (ADS)

Curry, Shannon M.; Luhmann, Janet; Ma, Yingjuan; Liemohn, Michael; Dong, Chuanfei; Hara, Takuya

2015-09-01

Without the shielding of a substantial intrinsic dipole magnetic field, the atmospheres of Mars and Venus are particularly susceptible to similar atmospheric ion energization and scavenging processes. However, each planet has different attributes and external conditions controlling its high altitude planetary ion spatial and energy distributions. This paper describes analogous test particle simulations in background MHD fields that allow us to compare the properties and fates, precipitation or escape, of the mainly O+ atmospheric pick-up ions at Mars and Venus. The goal is to illustrate how atmospheric and planetary scales affect the upper atmospheres and space environments of our terrestrial planet neighbors. The results show the expected convection electric field-related hemispheric asymmetries in both precipitation and escape, where the degree of asymmetry at each planet is determined by the planetary scale and local interplanetary field strength. At Venus, the kinetic treatment of O+ reveals a strong nightside source of precipitation while Mars' crustal fields complicate the simple asymmetry in ion precipitation and drive a dayside source of precipitation. The pickup O+ escape pattern at both Venus and Mars exhibits low energy tailward escape, but Mars exhibits a prominent, high energy 'polar plume' feature in the hemisphere of the upward convection electric field while the Venus ion wake shows only a modest poleward concentration. The overall escape is larger at Venus than Mars (2.1 ×1025 and 4.3 ×1024 at solar maximum, respectively), but the efficiency (likelihood) of O+ escaping is 2-3 times higher at Mars. The consequences of these comparisons for pickup ion related atmospheric energy deposition, loss rates, and detection on spacecraft including PVO, VEX, MEX and MAVEN are considered. In particular, both O+ precipitation and escape show electric field controlled asymmetries that grow with energy, while the O+ fluxes and energy spectra at selected spatial locations show characteristic signatures of the pickup related acceleration and precipitation.

33 CFR 211.13 - Approval of the Office of the Chief of Engineers.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Chief of Engineers. 211.13 Section 211.13 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE REAL ESTATE ACTIVITIES OF THE CORPS OF ENGINEERS IN CONNECTION... of the Office of the Chief of Engineers. In any case in which it may be necessary or advisable in the...

A Spatiotemporal Clustering Approach to Maritime Domain Awareness

DTIC Science & Technology

2013-09-01

1997. [25] M. E. Celebi, “Effective initialization of k-means for color quantization,” 16th IEEE International Conference on Image Processing (ICIP...release; distribution is unlimited 12b. DISTRIBUTION CODE 13. ABSTRACT (maximum 200 words) Spatiotemporal clustering is the process of grouping...Department of Electrical and Computer Engineering iv THIS PAGE INTENTIONALLY LEFT BLANK v ABSTRACT Spatiotemporal clustering is the process of

Late Veneer collisions and their impact on the evolution of Venus (PS Division Outstanding ECS Award Lecture)

NASA Astrophysics Data System (ADS)

Gillmann, Cedric; Golabek, Gregor; Tackley, Paul; Raymond, Sean

2017-04-01

During the end of the accretion, the so-called Late Veneer phase, while the bulk of the mass of terrestrial planets is already in place, a substantial number of large collisions can still occur. Those impacts are thought to be responsible for the repartition of the Highly Siderophile Elements. They are also susceptible to have a strong effect on volatile repartition and mantle convection. We study how Late Veneer impacts modify the evolution of Venus and its atmosphere, using a coupled numerical simulation. We focus on volatile exchanges and their effects on surface conditions. Mantle dynamics, volcanism and degassing processes lead to an input of gases in the atmosphere and are modeled using the StagYY mantle convection code. Volatile losses are estimated through atmospheric escape modeling. It involves two different aspects: hydrodynamic escape (0-500 Myr) and non-thermal escape. Hydrodynamic escape is massive but occurs only when the solar energy input is strong. Post 4 Ga escape from non-thermal processes is comparatively low but long-lived. The resulting state of the atmosphere is used to the calculate greenhouse effect and surface temperature, through a one-dimensional gray radiative-convective model. Large impacts are capable of contributing to (i) atmospheric escape, (ii) volatile replenishment and (iii) energy transfer to the mantle. We test various impactor compositions, impact parameters (velocity, location, size, and timing) and eroding power. Scenarios we tested are adapted from numerical stochastic simulations (Raymond et al., 2013). Impactor sizes are dominated by large bodies (R>500 km). Erosion of the atmosphere by a few large impacts appears limited. Swarms of smaller more mass-effective impactors seem required for this effect to be significant. Large impactors have two main effects on the atmosphere. They can (i) create a large input of volatile from the melting they cause during the impact and through the volatiles they carry. This leads to an increase in atmosphere density and surface temperatures. However, early impacts can also (ii) deplete the mantle of Venus and (assuming strong early escape) ultimately remove volatiles from the system, leading to lower late degassing and lower surface temperatures. The competition between those effects depends on the time of the impact, which directly governs the strength of atmospheric losses.

CMMI(Registered) for Development, Version 1.3

DTIC Science & Technology

2010-11-01

ISO /IEC 15288:2008 Systems and Software Engineering – System Life Cycle Processes [ ISO 2008b] ISO /IEC 27001 :2005 Information technology – Security...IEC 2005 International Organization for Standardization and International Electrotechnical Commission. ISO /IEC 27001 Information Technology...International Electrotechnical Commission ( ISO /IEC) body of standards. CMMs focus on improving processes in an organization. They contain the

Autonomous Soft Robotic Fish Capable of Escape Maneuvers Using Fluidic Elastomer Actuators.

PubMed

Marchese, Andrew D; Onal, Cagdas D; Rus, Daniela

2014-03-01

In this work we describe an autonomous soft-bodied robot that is both self-contained and capable of rapid, continuum-body motion. We detail the design, modeling, fabrication, and control of the soft fish, focusing on enabling the robot to perform rapid escape responses. The robot employs a compliant body with embedded actuators emulating the slender anatomical form of a fish. In addition, the robot has a novel fluidic actuation system that drives body motion and has all the subsystems of a traditional robot onboard: power, actuation, processing, and control. At the core of the fish's soft body is an array of fluidic elastomer actuators. We design the fish to emulate escape responses in addition to forward swimming because such maneuvers require rapid body accelerations and continuum-body motion. These maneuvers showcase the performance capabilities of this self-contained robot. The kinematics and controllability of the robot during simulated escape response maneuvers are analyzed and compared with studies on biological fish. We show that during escape responses, the soft-bodied robot has similar input-output relationships to those observed in biological fish. The major implication of this work is that we show soft robots can be both self-contained and capable of rapid body motion.

Multi-species analysis of ion distributions at Mars

NASA Astrophysics Data System (ADS)

Curry, S.; Liemohn, M. W.; Fang, X.; Ma, Y.; Johnson, B.; Bougher, S. W.; Dong, C.

2012-12-01

This study focuses on using the Mars Test Particle simulation to compare observations with virtual detections of O+, O2+, CO2+, and H+ in an orbital configuration in the Mars space environment. These planetary pick-up ions are formed when the solar wind directly interacts with the neutral atmosphere, causing the ions to be accelerated by the background convective electric field. The subsequent mass loading and ion escape are still the subject of great interest, specifically with respect to which species dominates ion loss from Mars. Modeling efforts and observations have found different results; some conclude that O+ is the most dominant escaping ion while others conclude that O2+ has the larger total loss rate. Furthermore, mass loss might actually favor CO2+ because of its tri-atomic structure. To address this unresolved issue, this study will present velocity space distributions for different species and discuss fluxes and escape rates using different modeling parameters. The simulation will also illustrate individual particle traces, which reveal the origin and trajectories of the different ion species. Finally, results from different solar conditions will be presented with respect to ion fluxes and energies as well as overall escape in order to robustly describe the physical processes controlling planetary ion distributions and atmospheric escape.

Speed kills: ineffective avian escape responses to oncoming vehicles

PubMed Central

DeVault, Travis L.; Blackwell, Bradley F.; Seamans, Thomas W.; Lima, Steven L.; Fernández-Juricic, Esteban

2015-01-01

Animal–vehicle collisions cause high levels of vertebrate mortality worldwide, and what goes wrong when animals fail to escape and ultimately collide with vehicles is not well understood. We investigated alert and escape behaviours of captive brown-headed cowbirds (Molothrus ater) in response to virtual vehicle approaches of different sizes and at speeds ranging from 60 to 360 km h−1. Alert and flight initiation distances remained similar across vehicle speeds, and accordingly, alert and flight initiation times decreased at higher vehicle speeds. Thus, avoidance behaviours in cowbirds appeared to be based on distance rather than time available for escape, particularly at 60–150 km h−1; however, at higher speeds (more than or equal to 180 km h−1) no trend in response behaviour was discernible. As vehicle speed increased, cowbirds did not have enough time to assess the approaching vehicle, and cowbirds generally did not initiate flight with enough time to avoid collision when vehicle speed exceeded 120 km h−1. Although potentially effective for evading predators, the decision-making process used by cowbirds in our study appears maladaptive in the context of avoiding fast-moving vehicles. Our methodological approach and findings provide a framework to assess how novel management strategies could affect escape rules, and the sensory and cognitive abilities animals use to avoid vehicle collisions. PMID:25567648

Standard CMMI (Registered Trademark) Appraisal Method for Process Improvement (SCAMPI (Service Mark)) A, Version 1.3: Method Definition Document

DTIC Science & Technology

2011-03-01

performance of Federal Government Contract Number FA8721-05- C -0003 with Carnegie Mellon University for the operation of the Software Engineering... C Roles and Responsibilities 195 Appendix D Reporting Requirements and Options 201 Appendix E Managed Discovery 203 Appendix F Scoping and...Upgrade Team (SUT) • Mary Busby , Lockheed Martin • Palma Buttles-Valdez, Software Engineering Institute • Paul Byrnes, Integrated System Diagnostics

Particle Methods for Electromagnetic Wave Propagation Problems

DTIC Science & Technology

2014-09-15

operation with respect to the underlying jump process. Using (8), (9), (12), (13), and the commutation of the expectation and differential operators (18...F’03) received the B.S. degree in electronics and communications engi- neering from REC-Warangal (now NIT Warangal), Warangal, India , in 1981, the...M.S. degree in mi- crowave and radar engineering from IIT-Kharagpur, Kharagpur, India , in 1983, and the Ph.D. degree in electrical engineering from the

46 CFR 13.505 - Proof of service for tankerman-engineer endorsement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Proof of service for tankerman-engineer endorsement. 13... SEAMEN CERTIFICATION OF TANKERMEN Requirements for Tankerman-Engineer Endorsement § 13.505 Proof of service for tankerman-engineer endorsement. (a) Service must be proved by a letter on company letterhead...

Gravity-assist engine for space propulsion

NASA Astrophysics Data System (ADS)

Bergstrom, Arne

2014-06-01

As a possible alternative to rockets, the present article describes a new type of engine for space travel, based on the gravity-assist concept for space propulsion. The new engine is to a great extent inspired by the conversion of rotational angular momentum to orbital angular momentum occurring in tidal locking between astronomical bodies. It is also greatly influenced by Minovitch's gravity-assist concept, which has revolutionized modern space technology, and without which the deep-space probes to the outer planets and beyond would not have been possible. Two of the three gravitating bodies in Minovitch's concept are in the gravity-assist engine discussed in this article replaced by an extremely massive ‘springbell' (in principle a spinning dumbbell with a powerful spring) incorporated into the spacecraft itself, and creating a three-body interaction when orbiting around a gravitating body. This makes gravity-assist propulsion possible without having to find suitably aligned astronomical bodies. Detailed numerical simulations are presented, showing how an actual spacecraft can use a ca 10-m diameter springbell engine in order to leave the earth's gravitational field and enter an escape trajectory towards interplanetary destinations.

Measurement of carbon capture efficiency and stored carbon leakage

DOEpatents

Keeling, Ralph F.; Dubey, Manvendra K.

2013-01-29

Data representative of a measured carbon dioxide (CO.sub.2) concentration and of a measured oxygen (O.sub.2) concentration at a measurement location can be used to determine whether the measured carbon dioxide concentration at the measurement location is elevated relative to a baseline carbon dioxide concentration due to escape of carbon dioxide from a source associated with a carbon capture and storage process. Optionally, the data can be used to quantify a carbon dioxide concentration increase at the first location that is attributable to escape of carbon dioxide from the source and to calculate a rate of escape of carbon dioxide from the source by executing a model of gas-phase transport using at least the first carbon dioxide concentration increase. Related systems, methods, and articles of manufacture are also described.

THE {sup 12}C/{sup 13}C RATIO ON TITAN FROM CASSINI INMS MEASUREMENTS AND IMPLICATIONS FOR THE EVOLUTION OF METHANE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mandt, Kathleen E.; Waite, J. Hunter; Teolis, Benjamin

2012-04-20

We have re-evaluated the Cassini Ion Neutral Mass Spectrometer (INMS) {sup 12}C/{sup 13}C ratios in the upper atmosphere of Titan based on new calibration sensitivities and an improved model for the NH{sub 3} background in the {sup 13}CH{sub 4} mass channel. The INMS measurements extrapolated to the surface give a {sup 12}C/{sup 13}C in CH{sub 4} of 88.5 {+-} 1.4. We compare the results to a revised ratio of 91.1 {+-} 1.4 provided by the Huygens Gas Chromatograph Mass Spectrometer and 86.5 {+-} 7.9 provided by the Cassini Infrared Spectrometer and determine implications of the revised ratios for the evolutionmore » of methane in Titan's atmosphere. Because the measured {sup 12}C/{sup 13}C is within the probable range of primordial values, we can only determine an upper boundary for the length of time since methane began outgassing from the interior, assuming that outgassing of methane (e.g., cryovolcanic activity) has been continuous ever since. We find that three factors play a crucial role in this timescale: (1) the escape rate of methane, (2) the difference between the current and initial ratios and the rate of methane, and (3) production or resupply due to cryovolcanic activity. We estimate an upper limit for the outgassing timescale of 470 Myr. This duration can be extended to 940 Myr if production rates are large enough to counteract the fractionation due to escape and photochemistry. There is no lower limit to the timescale because the current ratios are within the range of possible primordial values.« less

INMS Titan Observations

NASA Astrophysics Data System (ADS)

Waite, J. H., Jr.; Niemann, H.; Yelle, R. V.; Kasprzak, W. T.; Cravens, T. E.; Luhmann, J. G.; McNutt, R. L.; Ip, W.-H.; De La Haye, V.; Ledvina, S.; Mueller-Wordarg, I.; Borggren, N.

2005-08-01

The Ion Neutral Mass Spectrometer (INMS) aboard the Cassini Orbiter has obtained the first in situ composition measurements of the neutral densities of molecular nitrogen, methane, hydrogen, argon, and a host of stable carbon-nitrile ion and neutral compounds in the first and sixth flybys of Titan. The bulk composition and thermal structure of the moon's upper atmosphere appear to vary with latitude and local time. The new data set provides strong evidence for atmospheric waves in the upper atmosphere and for the existence of a warm, chemically complex corona. Furthermore, the data set provides direct measurements of isotopes of nitrogen, carbon, and argon, which reveal interesting clues about the evolution of the atmosphere. The atmosphere likely formed from outgassing as planetesimals composed of silicates, water ice, clathrates of methane, and ammonia hydrates coalesced. Subsequent photochemistry and/or shock-induced chemistry likely converted the atmospheric nitrogen into molecular nitrogen, which is inferred by the absence (<0.6 ppm) of 36Ar in the INMS data. (Ice clathrate delivery of N2 would have presumably also delivered 36Ar to the proto Titan.) The decrease of the 14N to 15N isotopic ratio with respect to the terrestrial value allows us to suggest an early atmosphere >1.5 to 100 times more substantial that was lost via escape over the intervening 4.5 billion years. Carbon in the form of methane has continued to outgas over time from the interior (as inferred from the elevated 12C to 13C ratio as compared to terrestrial values) with much of its subsequent photolysis products being deposited in the form of complex hydrocarbons on the surface ( 5 x 1027 s-1 as estimated from the H2 escape rate of 6.1 ± 0.2 x 109 cm-2 s-1 measured by INMS). This talk will highlight the composition, vertical structure, wave processes, and escape of Titan's atmosphere.

Social encapsulation of beetle parasites by Cape honeybee colonies (Apis mellifera capensis Esch.)

NASA Astrophysics Data System (ADS)

Neumann, P.; Pirk, C. W. W.; Hepburn, H. R.; Solbrig, A. J.; Ratnieks, F. L. W.; Elzen, P. J.; Baxter, J. R.

2001-05-01

Worker honeybees (Apis mellifera capensis) encapsulate the small hive beetle (Aethina tumida), a nest parasite, in propolis (tree resin collected by the bees). The encapsulation process lasts 1-4 days and the bees have a sophisticated guarding strategy for limiting the escape of beetles during encapsulation. Some encapsulated beetles died (4.9%) and a few escaped (1.6%). Encapsulation has probably evolved because the small hive beetle cannot easily be killed by the bees due to its hard exoskeleton and defensive behaviour.

A model for the evolution of CO2 on Mars

NASA Technical Reports Server (NTRS)

Haberle, Robert M.; Tyler, D.; Mckay, C. P.; Davis, W. L.

1993-01-01

Our MSATT work has focused on the evolution of CO2 on Mars. We have constructed a model that predicts the evolution of CO2 on Mars from a specified initial amount at the end of the heavy bombardment to the present. The model draws on published estimates of the main process believed to affect the fate of CO2 during this period: chemical weathering, regolith uptake, polar cap formation, and atmospheric escape. Except for escape, the rate at which these processes act is controlled by surface temperatures that we calculate using a modified version of the Gierasch and Toon energy balance model. Various aspects of this work are covered.

46 CFR 113.35-13 - Mechanical engine order telegraph systems; operation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Mechanical engine order telegraph systems; operation. 113.35-13 Section 113.35-13 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED...-13 Mechanical engine order telegraph systems; operation. If more than one transmitter operates a...

Direct observation of the carrier transport process in InGaN quantum wells with a pn-junction

NASA Astrophysics Data System (ADS)

Wu, Haiyan; Ma, Ziguang; Jiang, Yang; Wang, Lu; Yang, Haojun; Li, Yangfeng; Zuo, Peng; Jia, Haiqiang; Wang, Wenxin; Zhou, Junming; Liu, Wuming; Chen, Hong

2016-11-01

A new mechanism of light-to-electricity conversion that uses InGaN/GaN QWs with a p-n junction is reported. According to the well established light-to-electricity conversion theory, quantum wells (QWs) cannot be used in solar cells and photodetectors because the photogenerated carriers in QWs usually relax to ground energy levels, owing to quantum confinement, and cannot form a photocurrent. We observe directly that more than 95% of the photoexcited carriers escape from InGaN/GaN QWs to generate a photocurrent, indicating that the thermionic emission and tunneling processes proposed previously cannot explain carriers escaping from QWs. We show that photoexcited carriers can escape directly from the QWs when the device is under working conditions. Our finding challenges the current theory and demonstrates a new prospect for developing highly efficient solar cells and photodetectors. Project supported by the National Natural Science Foundation of China (Grant Nos. 11574362, 61210014, and 11374340) and the Innovative Clean-energy Research and Application Program of Beijing Municipal Science and Technology Commission, China (Grant No. Z151100003515001).

Recovery of exhaust waste heat for a hybrid car using steam turbine

NASA Astrophysics Data System (ADS)

Ababatin, Yasser

A number of car engines operate with an efficiency rate of approximately 22% to 25% [1]. The remainder of the energy these engines generate is wasted through heat escape out of the exhaust pipe. There is now an increasing desire to reuse this heat energy, which would improve the overall efficiency of car engines by reducing their consumption of fuel. Another benefit is that such reuse would minimize harmful greenhouse gases that are emitted into the environment. Therefore, the purpose of this project is to examine how the wasted heat energy can be reused and/or recovered by use of a heat recovery system that would store this energy in a hybrid car battery. Green turbines will be analyzed as a possible solution to recycle the lost energy in a way that will also improve the overall automotive energy efficiency.

Site-Specific Editing of the Plasmodium falciparum Genome Using Engineered Zinc-Finger Nucleases

PubMed Central

Straimer, Judith; Lee, Marcus CS; Lee, Andrew H; Zeitler, Bryan; Williams, April E; Pearl, Jocelynn R; Zhang, Lei; Rebar, Edward J; Gregory, Philip D; Llinás, Manuel; Urnov, Fyodor D; Fidock, David A

2013-01-01

Malaria afflicts over 200 million people worldwide and its most lethal etiologic agent, Plasmodium falciparum, is evolving to resist even the latest-generation therapeutics. Efficient tools for genome-directed investigations of P. falciparum pathogenesis, including drug resistance mechanisms, are clearly required. Here we report rapid and targeted genetic engineering of this parasite, using zinc-finger nucleases (ZFNs) that produce a double-strand break in a user-defined locus and trigger homology-directed repair. Targeting an integrated egfp locus, we obtained gene deletion parasites with unprecedented speed (two weeks), both with and without direct selection. ZFNs engineered against the endogenous parasite gene pfcrt, responsible for chloroquine treatment escape, rapidly produced parasites that carried either an allelic replacement or a panel of specified point mutations. The efficiency, versatility and precision of this method will enable a diverse array of genome editing approaches to interrogate this human pathogen. PMID:22922501

Precision measurement of the positron asymmetry of laser-cooled, spin-polarized 37K

NASA Astrophysics Data System (ADS)

Melconian, Dan; Fenker, B.; Behr, J. A.; Anholm, M.; Ashery, D.; Behling, R. S.; Cohen, I.; Craiciu, I.; Gorelov, A.; Gwinner, G.; McNeil, J.; Mehlman, M.; Smale, S.; Warner, C. L.

2017-01-01

Precision low-energy measurements in nuclear β decay can be used to provide constraints on possible physics beyond the standard model, complementing searches at high-energy colliders. The short-lived isotope 37K was produced at ISAC-TRIUMF and confined in an alternating magneto-optical trap before being spin-polarized to 99.13(9)% via optical pumping. Our system allows for an exceptionally open geometry with the decay products escaping with their momenta unperturbed by the shallow trapping potential. The emitted positrons are detected in a pair of symmetric detectors placed along the polarization axis to measure the β asymmetry. The analysis was performed blind and considers β-scattering as well as other systematic effects. The results place limits on the mass of a hypothetical W boson coupling to right-handed neutrinos as well as contribute to an independent determination of the Vud element of the CKM matrix. The β asymmetry result as well as improvements and future plans will be described. This work is supported in part by the U.S. Department of Energy, the Natural Sciences and Engineering Research Council of Canada, and the Israel Science Foundation.

Exposure to Melan-A/MART-126-35 tumor epitope specific CD8+T cells reveals immune escape by affecting the ubiquitin-proteasome system (UPS)

PubMed Central

Ebstein, Frédéric; Keller, Martin; Paschen, Annette; Walden, Peter; Seeger, Michael; Bürger, Elke; Krüger, Elke; Schadendorf, Dirk; Kloetzel, Peter-M.; Seifert, Ulrike

2016-01-01

Efficient processing of target antigens by the ubiquitin-proteasome-system (UPS) is essential for treatment of cancers by T cell therapies. However, immune escape due to altered expression of IFN-γ-inducible components of the antigen presentation machinery and consequent inefficient processing of HLA-dependent tumor epitopes can be one important reason for failure of such therapies. Here, we show that short-term co-culture of Melan-A/MART-1 tumor antigen-expressing melanoma cells with Melan-A/MART-126-35-specific cytotoxic T lymphocytes (CTL) led to resistance against CTL-induced lysis because of impaired Melan-A/MART-126-35 epitope processing. Interestingly, deregulation of p97/VCP expression, which is an IFN-γ-independent component of the UPS and part of the ER-dependent protein degradation pathway (ERAD), was found to be essentially involved in the observed immune escape. In support, our data demonstrate that re-expression of p97/VCP in Melan-A/MART-126-35 CTL-resistant melanoma cells completely restored immune recognition by Melan-A/MART-126-35 CTL. In conclusion, our experiments show that impaired expression of IFN-γ-independent components of the UPS can exert rapid immune evasion of tumor cells and suggest that tumor antigens processed by distinct UPS degradation pathways should be simultaneously targeted in T cell therapies to restrict the likelihood of immune evasion due to impaired antigen processing. PMID:27143649

Nitrogen Isotopic Composition of Organic Matter in a Pristine Collection IDP

NASA Technical Reports Server (NTRS)

Messenger, S.; Nakamura-Messenger, K.; Keller, L. P.; Clemett, S. J.; Nguyen, A. N.; Walker, Robert M.

2012-01-01

Anhydrous chondritic porous interplanetary dust particles (CP IDPs) are probable cometary materials that show primitive characteristics, such as unequilibrated mineralogy, fragile structure, and abundant presolar grains and organic matter [1-3]. CP IDPs are richer in aliphatic species and N-bearing aromatic hydrocarbons than meteoritic organics and commonly exhibit highly anomalous H and N isotopic compositions [4,5]. Cometary organic matter is of interest in part because it has escaped the hydrothermal processing experienced by meteorites. However, IDPs are collected using silicon oil that must be removed with strong organic solvents such as hexane. This procedure is likely to have removed some fraction of soluble organic phases in IDPs. We recently reported the first stratospheric collection of IDPs without the use of silicone oil [6]. Here we present initial studies of the carbonaceous material in an IDP from this collection.

Mechanical Engineering Department engineering research: Annual report, FY 1986

DOE Office of Scientific and Technical Information (OSTI.GOV)

Denney, R.M.; Essary, K.L.; Genin, M.S.

1986-12-01

This report provides information on the five areas of research interest in LLNL's Mechanical Engineering Department. In Computer Code Development, a solid geometric modeling program is described. In Dynamic Systems and Control, structure control and structure dynamics are discussed. Fabrication technology involves machine cutting, interferometry, and automated optical component manufacturing. Materials engineering reports on composite material research and measurement of molten metal surface properties. In Nondestructive Evaluation, NMR, CAT, and ultrasound machines are applied to manufacturing processes. A model for underground collapse is developed. Finally, an alternative heat exchanger is investigated for use in a fusion power plant. Separate abstractsmore » were prepared for each of the 13 reports in this publication. (JDH)« less

Selection of antigenically advanced variants of seasonal influenza viruses

PubMed Central

Ozawa, Makoto; Taft, Andrew S.; Das, Subash C.; Hanson, Anthony P.; Song, Jiasheng; Imai, Masaki; Wilker, Peter R.; Watanabe, Tokiko; Watanabe, Shinji; Ito, Mutsumi; Iwatsuki-Horimoto, Kiyoko; Russell, Colin A.; James, Sarah L.; Skepner, Eugene; Maher, Eileen A.; Neumann, Gabriele; Kelso, Anne; McCauley, John; Wang, Dayan; Shu, Yuelong; Odagiri, Takato; Tashiro, Masato; Xu, Xiyan; Wentworth, David E.; Katz, Jacqueline M.; Cox, Nancy J.; Smith, Derek J.; Kawaoka, Yoshihiro

2016-01-01

Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. Further, we selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014–2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature. PMID:27572841

Escape problem under stochastic volatility: The Heston model

NASA Astrophysics Data System (ADS)

Masoliver, Jaume; Perelló, Josep

2008-11-01

We solve the escape problem for the Heston random diffusion model from a finite interval of span L . We obtain exact expressions for the survival probability (which amounts to solving the complete escape problem) as well as for the mean exit time. We also average the volatility in order to work out the problem for the return alone regardless of volatility. We consider these results in terms of the dimensionless normal level of volatility—a ratio of the three parameters that appear in the Heston model—and analyze their form in several asymptotic limits. Thus, for instance, we show that the mean exit time grows quadratically with large spans while for small spans the growth is systematically slower, depending on the value of the normal level. We compare our results with those of the Wiener process and show that the assumption of stochastic volatility, in an apparently paradoxical way, increases survival and prolongs the escape time. We finally observe that the model is able to describe the main exit-time statistics of the Dow-Jones daily index.

Outgassing History and Escape of the Martian Atmosphere and Water Inventory

NASA Astrophysics Data System (ADS)

Lammer, Helmut; Chassefière, Eric; Karatekin, Özgür; Morschhauser, Achim; Niles, Paul B.; Mousis, Olivier; Odert, Petra; Möstl, Ute V.; Breuer, Doris; Dehant, Véronique; Grott, Matthias; Gröller, Hannes; Hauber, Ernst; Pham, Lê Binh San

2013-01-01

The evolution and escape of the martian atmosphere and the planet's water inventory can be separated into an early and late evolutionary epoch. The first epoch started from the planet's origin and lasted ˜500 Myr. Because of the high EUV flux of the young Sun and Mars' low gravity it was accompanied by hydrodynamic blow-off of hydrogen and strong thermal escape rates of dragged heavier species such as O and C atoms. After the main part of the protoatmosphere was lost, impact-related volatiles and mantle outgassing may have resulted in accumulation of a secondary CO2 atmosphere of a few tens to a few hundred mbar around ˜4-4.3 Gyr ago. The evolution of the atmospheric surface pressure and water inventory of such a secondary atmosphere during the second epoch which lasted from the end of the Noachian until today was most likely determined by a complex interplay of various nonthermal atmospheric escape processes, impacts, carbonate precipitation, and serpentinization during the Hesperian and Amazonian epochs which led to the present day surface pressure.

Achieving Space Shuttle Abort-to-Orbit Using the Five-Segment Booster

NASA Technical Reports Server (NTRS)

Craft, Joe; Ess, Robert; Sauvageau, Don

2003-01-01

The Five-Segment Booster design concept was evaluated by a team that determined the concept to be feasible and capable of achieving the desired abort-to-orbit capability when used in conjunction with increased Space Shuttle main engine throttle capability. The team (NASA Johnson Space Center, NASA Marshall Space Flight Center, ATK Thiokol Propulsion, United Space Alliance, Lockheed-Martin Space Systems, and Boeing) selected the concept that provided abort-to-orbit capability while: 1) minimizing Shuttle system impacts by maintaining the current interface requirements with the orbiter, external tank, and ground operation systems; 2) minimizing changes to the flight-proven design, materials, and processes of the current four-segment Shuttle booster; 3) maximizing use of existing booster hardware; and 4) taking advantage of demonstrated Shuttle main engine throttle capability. The added capability can also provide Shuttle mission planning flexibility. Additional performance could be used to: enable implementation of more desirable Shuttle safety improvements like crew escape, while maintaining current payload capability; compensate for off nominal performance in no-fail missions; and support missions to high altitudes and inclinations. This concept is a low-cost, low-risk approach to meeting Shuttle safety upgrade objectives. The Five-Segment Booster also has the potential to support future heavy-lift missions.

Use of Dual Frequency Identification Sonar to Determine Adult Chinook Salmon (Oncorhynchus tshawytscha) Escapement in the Secesh River, Idaho ; Annual Report, January 2008 – December 2008.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kucera, Paul A.

2009-06-26

Chinook salmon in the Snake River basin were listed as threatened under the Endangered Species Act in 1992 (NMFS 1992). The Secesh River represents the only stream in the Snake River basin where natural origin (wild) salmon escapement monitoring occurs at the population level, absent a supplementation program. As such the Secesh River has been identified as a long term salmon escapement and productivity monitoring site by the Nez Perce Tribe Department of Fisheries Resources Management. Salmon managers will use this data for effective population management and evaluation of the effect of conservation actions on a natural origin salmon population.more » The Secesh River also acts as a reference stream for supplementation program comparison. Dual frequency identification sonar (DIDSON) was used to determine adult spring and summer Chinook salmon escapement in the Secesh River in 2008. DIDSON technology was selected because it provided a non-invasive method for escapement monitoring that avoided listed species trapping and handling incidental mortality, and fish impedance related concerns. The DIDSON monitoring site was operated continuously from June 13 to September 14. The first salmon passage was observed on July 3. DIDSON site total estimated salmon escapement, natural and hatchery fish, was 888 fish {+-} 65 fish (95% confidence interval). Coefficient of variation associated with the escapement estimate was 3.7%. The DIDSON unit was operational 98.1% of the salmon migration period. Adult salmon migration timing in the Secesh River occurred over 74 days from July 3 to September 14, with 5,262 total fish passages observed. The spawning migration had 10%, median, and 90% passage dates of July 8, July 16, and August 12, respectively. The maximum number of net upstream migrating salmon was above the DIDSON monitoring site on August 27. Validation monitoring of DIDSON target counts with underwater optical cameras occurred for species identification. A total of 860 optical camera identified salmon passage observations were identical to DIDSON target counts. However, optical cameras identified eight jack salmon (3 upstream, 5 downstream) less than 55 cm in length that DIDSON did not count as salmon because of the length criteria employed ({ge} 55 cm). Precision of the DIDSON technology was evaluated by comparing estimated net upstream salmon escapement and associated 95% confidence intervals between two DIDSON sonar units operated over a five day period. The DIDSON 1 salmon escapement was 145.7 fish ({+-} 2.3), and the DIDSON 2 escapement estimate was 150.5 fish ({+-} 5). The overlap in the 95% confidence intervals suggested that the two escapement estimates were not significantly different from each other. Known length salmon carcass trials were conducted in 2008 to examine the accuracy of manually measured lengths, obtained using DIDSON software, on high frequency files at a 5 m window length. Linear regression demonstrated a highly significant relationship between known lengths and manually measured salmon carcass lengths (p < 0.0001). A positive bias in manual length measurement of 6.8% to 8% existed among the two observers in the analysis. Total Secesh River salmon escapement (natural origin and hatchery) in 2008 was 912 fish. Natural origin salmon escapement in the entire Secesh River drainage was 847 fish. The estimated natural origin spawner abundance was 836 fish. Salmon spawner abundance in 2008 increased by three fold compared to 2007 abundance levels. The 10 year geometric mean natural origin spawner abundance was 538 salmon and was below the recommended viable population threshold level established by the ICTRT (2007). One additional Snake River basin salmon population was assessed for comparison of natural origin salmon spawner abundance. The Johnson Creek/EFSF Salmon River population had a 10 year geometric mean natural origin spawner abundance of 254 salmon. Salmon spawner abundance levels in both streams were below viable population thresholds. DIDSON technology has been used in the Secesh River to determine salmon escapement over the past five years. The results suggest that DIDSON technology is reliable and can be used to generate accurate and precise estimates of salmon escapement if appropriate methods are used.« less

Physical Processes for Driving Ionospheric Outflows in Global Simulations

NASA Technical Reports Server (NTRS)

Moore, Thomas Earle; Strangeway, Robert J.

2009-01-01

We review and assess the importance of processes thought to drive ionospheric outflows, linking them as appropriate to the solar wind and interplanetary magnetic field, and to the spatial and temporal distribution of their magnetospheric internal responses. These begin with the diffuse effects of photoionization and thermal equilibrium of the ionospheric topside, enhancing Jeans' escape, with ambipolar diffusion and acceleration. Auroral outflows begin with dayside reconnexion and resultant field-aligned currents and driven convection. These produce plasmaspheric plumes, collisional heating and wave-particle interactions, centrifugal acceleration, and auroral acceleration by parallel electric fields, including enhanced ambipolar fields from electron heating by precipitating particles. Observations and simulations show that solar wind energy dissipation into the atmosphere is concentrated by the geomagnetic field into auroral regions with an amplification factor of 10-100, enhancing heavy species plasma and gas escape from gravity, and providing more current carrying capacity. Internal plasmas thus enable electromagnetic driving via coupling to the plasma, neutral gas and by extension, the entire body " We assess the Importance of each of these processes in terms of local escape flux production as well as global outflow, and suggest methods for their implementation within multispecies global simulation codes. We complete 'he survey with an assessment of outstanding obstacles to this objective.

Predictive Engineering Tools for Injection-Molded Long-Carbon-Fiber Thermoplastic Composites - FY13 Third Quarterly Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Ba Nghiep; Simmons, Kevin L.

2013-08-06

This quarterly report summarizes the status for the project planning to obtain all the approvals required for a Cooperative Research and Development Agreement (CRADA) with Autodesk, Inc., Toyota Motor Engineering and Manufacturing North America (Toyota), and Magna Exterior and Interiors Corporation (Magna). The CRADA documents have been processed by PNNL Legal Services that is also coordinating the revision effort with the industrial parties to address DOE’s comments.

Flight mechanics and control of escape manoeuvres in hummingbirds. II. Aerodynamic force production, flight control and performance limitations.

PubMed

Cheng, Bo; Tobalske, Bret W; Powers, Donald R; Hedrick, Tyson L; Wang, Yi; Wethington, Susan M; Chiu, George T-C; Deng, Xinyan

2016-11-15

The superior manoeuvrability of hummingbirds emerges from complex interactions of specialized neural and physiological processes with the unique flight dynamics of flapping wings. Escape manoeuvring is an ecologically relevant, natural behaviour of hummingbirds, from which we can gain understanding into the functional limits of vertebrate locomotor capacity. Here, we extend our kinematic analysis of escape manoeuvres from a companion paper to assess two potential limiting factors of the manoeuvring performance of hummingbirds: (1) muscle mechanical power output and (2) delays in the neural sensing and control system. We focused on the magnificent hummingbird (Eugenes fulgens, 7.8 g) and the black-chinned hummingbird (Archilochus alexandri, 3.1 g), which represent large and small species, respectively. We first estimated the aerodynamic forces, moments and the mechanical power of escape manoeuvres using measured wing kinematics. Comparing active-manoeuvring and passive-damping aerodynamic moments, we found that pitch dynamics were lightly damped and dominated by the effect of inertia, while roll dynamics were highly damped. To achieve observed closed-loop performance, pitch manoeuvres required faster sensorimotor transduction, as hummingbirds can only tolerate half the delay allowed in roll manoeuvres. Accordingly, our results suggested that pitch control may require a more sophisticated control strategy, such as those based on prediction. For the magnificent hummingbird, we estimated that escape manoeuvres required muscle mass-specific power 4.5 times that during hovering. Therefore, in addition to the limitation imposed by sensorimotor delays, muscle power could also limit the performance of escape manoeuvres. © 2016. Published by The Company of Biologists Ltd.

Do Lyman-alpha photons escape from star-forming galaxies through dust-holes?

NASA Astrophysics Data System (ADS)

Wofford, Aida

2012-10-01

The hydrogen Lyman-alpha line is arguably the most important signature of galaxies undergoing their first violent burst of star formation. Although Lya photons are easily destroyed by dust, candidate Lya emitters have been detected at z>5. Thus the line can potentially be used to probe galaxy formation and evolution, as long as the astrophysical processes that regulate the escape of Lya photons from star-forming galaxies are well understood.We request 15 orbits for imaging in Lya and the FUV continuum with ACS/SBC, and in the H-beta/H-alpha ratio {proxy for dust extinction} with WFC3/UVIS, a sample of isolated non-AGN face-on spirals for which our team previously obtained and analyzed COS FUV spectroscopy of the central regions. Each target shows a different Lya profile, i.e., pure absorption, P-Cygni like, and multiple-emission. From the COS data, we already know the starburst phase and H I gas velocity. The images would greatly increase the impact of our spectroscopic study by enabling us to 1} conclusively determine if Lya photons escape through dust-holes, 2} assess the relative importance of dust extinction, ISM kinematics, and starburst phase in regulating the Lya escape, 3} clarify what we can really learn from the Lya equivalent width, and 4} provide constraints on the dust extinction to Lya 3D radiative transfer models. Ultimately this program will inform our understanding of the Lya escape at high redshift by providing spatially resolved views of the local conditions within star-forming galaxies that favor escape.

A Spectroscopic Search for Leaking Lyman Continuum at Zeta Approximately 0.7

NASA Technical Reports Server (NTRS)

Bridge, Carrie R.; Teplitz, Harry I.; Siana, Brian; Scarlata, Claudia; Rudie, Gwen C.; Colbert, James; Ferguson, Henry C.; Brown, Thomas M.; Conselice, Christopher J.; Armus, Lee;

Software Reviews Since Acquisition Reform - The Artifact Perspective

DTIC Science & Technology

2004-01-01

Risk Management OLD NEW Slide 13Acquisition of Software Intensive Systems 2004 – Peter Hantos Single, basic software paradigm Single processor Low...software risk mitigation related trade-offs must be done together Integral Software Engineering Activities Process Maturity and Quality Frameworks Quality

Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles.

PubMed

Wang, Ming; Zuris, John A; Meng, Fantao; Rees, Holly; Sun, Shuo; Deng, Pu; Han, Yong; Gao, Xue; Pouli, Dimitra; Wu, Qi; Georgakoudi, Irene; Liu, David R; Xu, Qiaobing

2016-03-15

A central challenge to the development of protein-based therapeutics is the inefficiency of delivery of protein cargo across the mammalian cell membrane, including escape from endosomes. Here we report that combining bioreducible lipid nanoparticles with negatively supercharged Cre recombinase or anionic Cas9:single-guide (sg)RNA complexes drives the electrostatic assembly of nanoparticles that mediate potent protein delivery and genome editing. These bioreducible lipids efficiently deliver protein cargo into cells, facilitate the escape of protein from endosomes in response to the reductive intracellular environment, and direct protein to its intracellular target sites. The delivery of supercharged Cre protein and Cas9:sgRNA complexed with bioreducible lipids into cultured human cells enables gene recombination and genome editing with efficiencies greater than 70%. In addition, we demonstrate that these lipids are effective for functional protein delivery into mouse brain for gene recombination in vivo. Therefore, the integration of this bioreducible lipid platform with protein engineering has the potential to advance the therapeutic relevance of protein-based genome editing.

Spreading dynamic of viscous volcanic ash in stimulated jet engine conditions

NASA Astrophysics Data System (ADS)

song, wenjia; Lavallée, Yan; Hess, Kai-Uwe; Kueppers, Ulrich; Cimarelli, Corrado

2016-04-01

The ingestion of volcanic ash is widely recognised as a potentially fatal hazard for aircraft operation. The volcanic ash deposition process in a jet turbine is potentially complex. Volcanic ash in the air stream enters the inner liners of the combustors and partially or completely melts under the flames up to 2000 °C, at which point part of the ash deposits in the combustor fuel nozzle. Molten volcanic particles within high energy airflow escape the combustor to enter the turbine and impact the stationary (e.g., inlet nozzle guide vanes) and rotating airfoils (e.g., first stage high-pressure turbine blades) at high speed (up to Mach 1.25) in different directions, with the result that ash may stick, flow and remain liquid or solidify. Thus, the wetting behaviour of molten volcanic ash particle is fundamental to investigate impingement phenomena of ash droplet on the surface of real jet engine operation. The topic of wetting has received tremendous interest from both fundamental and applied points of view. However, due to the interdisciplinary gap between jet engine engineering and geology science, explicit investigation of wetting behaviour of volcanic ash at high temperature is in its infancy. We have taken a big step towards meeting this challenge. Here, we experimentally and theoretically investigate the wetting behaviour of viscous volcanic ash over a wide temperature range from 1100 to 1550 °C using an improved sessile-drop method. The results of our experiment demonstrate that temperature and viscosity play a critical role in determining the wetting possibility and governing the spreading kinetics of volcanic ash at high temperatures. Our systemic analysis of spreading of molten volcanic ash systems allows us to report on the fundamental differences between the mechanisms controlling spreading of organic liquids at room temperature and molten volcanic ash droplets.

Stellar Ablation of Planetary Atmospheres

NASA Technical Reports Server (NTRS)

Moore, Thomas E.; Horwitz, J. L.

2007-01-01

We review observations and theories of the solar ablation of planetary atmospheres, focusing on the terrestrial case where a large magnetosphere holds off the solar wind, so that there is little direct atmospheric impact, but also couples the solar wind electromagnetically to the auroral zones. We consider the photothermal escape flows known as the polar wind or refilling flows, the enhanced mass flux escape flows that result from localized solar wind energy dissipation in the auroral zones, and the resultant enhanced neutral atom escape flows. We term these latter two escape flows the "auroral wind." We review observations and theories of the heating and acceleration of auroral winds, including energy inputs from precipitating particles, electromagnetic energy flux at magnetohydrodynamic and plasma wave frequencies, and acceleration by parallel electric fields and by convection pickup processes also known as "centrifugal acceleration." We consider also the global circulation of ionospheric plasmas within the magnetosphere, their participation in magnetospheric disturbances as absorbers of momentum and energy, and their ultimate loss from the magnetosphere into the downstream solar wind, loading reconnection processes that occur at high altitudes near the magnetospheric boundaries. We consider the role of planetary magnetization and the accumulating evidence of stellar ablation of extrasolar planetary atmospheres. Finally, we suggest and discuss future needs for both the theory and observation of the planetary ionospheres and their role in solar wind interactions, to achieve the generality required for a predictive science of the coupling of stellar and planetary atmospheres over the full range of possible conditions.

Hydrogen in the Methanol Production Process

ERIC Educational Resources Information Center

Kralj, Anita Kovac; Glavic, Peter

2006-01-01

Hydrogen is a very important industrial gas in chemical processes. It is very volatile; therefore, it can escape from the process units and its mass balance is not always correct. In many industrial processes where hydrogen is reacted, kinetics are often related to hydrogen pressure. The right thermodynamic properties of hydrogen can be found for…

Industrial Test of High Strength Steel Plates Free Boron Q890D Used for Engineering Machinery

NASA Astrophysics Data System (ADS)

Dong, Ruifeng; Liu, Zetian; Gao, Jun

The chemistry composition, process parameters and the test results of Q890D free boron high strength steel plate used for engineering machinery was studied. The 16 40 mm thickness steel plates with good mechanical properties that was yield strength of 930 970 MPa, tensile strength of 978 1017 MPa, elongation of 13.5 15%, the average impact energy value of more than 100 J were developed by improving steel purity, adopting the reasonable controlled rolling and cooling process, using reasonable off-line quenching and tempering process. The test plates have good crack resistance in 60 °C preheat temperature condition because of that there are no any cracks in the surfaces, cross-section and roots of welding joints.

Evaluation of the StressWave Cold Working (SWCW) Process on High-Strength Aluminum Alloys for Aerospace

DTIC Science & Technology

2009-02-01

Alloy Spot- welds by Cold Working,” 13 International Pacific Conference on Automotive Engineering (IPC-13), Gyeongju, Korea, August 2005. 7. Kim...so that it remains normal to the indenting direction. The restraint provided around the area to be cold worked minimizes surface upset (albeit...direction. The restraint provided around the area to be cold worked minimizes surface upset (albeit small without a PF). The stabilizing aspect

46 CFR 13.501 - Original application for tankerman-engineer endorsement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 1 2014-10-01 2014-10-01 false Original application for tankerman-engineer endorsement... AND SEAMEN CERTIFICATION OF TANKERMEN Requirements for Tankerman-Engineer Endorsement § 13.501 Original application for tankerman-engineer endorsement. Each applicant for a tankerman-engineer...

STS-29 MS Bagian during post landing egress exercises in JSC FFT mockup

NASA Image and Video Library

1988-05-26

S88-38355 (27 May 1988) --- Astronaut James P. Bagian lowers himself from the top of one of the full-fuselage trainer in JSC's Shuttle mockup and integration laboratory during a post-landing, over-the-top emergency egress test. Bagian, a M.D., and one of three mission specialists assigned to NASA STS-29 flight of the Discovery, is working with engineers evaluating egress using the new crew escape equipment that includes a parachute harness.

Annual Systems Engineering Conference: Focusing on Improving Performance of Defense Systems Programs (10th). Volume 3. Thursday Presentations

DTIC Science & Technology

2007-10-25

the Phit <.0001 requirement) restricts tactical delivery conditions, the probability of a fragment hit may be further qualified by considering only...Pkill – UK uses “self damage” metric • Risk Analysis: “If the above procedures ( Phit or Pkill <.0001) still result in restricting tactical delivery...10 (From NAWCWD Briefing) 4 Safe Escape Analysis Requirements Calculate Phit ,Pkill, and Pdet Is Phit <= .0001 for all launch conditions Done NO YES

Assessment of the Effectiveness of Clay Soil Covers as Engineered Barriers in Waste Disposal Facilities with Emphasis on Modeling Cracking Behavior

DTIC Science & Technology

2008-06-01

escaping the clay and keeping its compacted conditions constant. Other stabilizing additives such as surfactants or cement and applications such as foamed ...not a local phenomenon. Once a crack is formed, increasing the width of the crack at the surface by additional shrinkage will also extend the depth...at the surface, increasing the width of the crack by additional shrinkage will drive the crack deeper into the soil mass, expos- ing new surfaces to

Air Superiority and Airfield Attack - Lessons from History.

DTIC Science & Technology

1984-05-15

escaped the port of Dunkirk and the Luftwaffe had failed miserably to fulfill Giring’s unwarranted boast, "My.Luftwaffe will do it alone.",25 In the forty...AMIENS + CHERBOURG LE HARV" le11 0+ + Figure 2. Combat environment. 35 a single-engine fighter with a flashing fuel-warning light or oil streaming...Brussels-Evere (c) Brussels-Grimbergen (d) Brussels-Melsbrock (e) Antwerp-Durne (f) St. Trond (g) Le Culot (h) Asch (2) Holland (a) Eindhoven, and

Versatile de novo enzyme activity in capsid proteins from an engineered M13 bacteriophage library.

PubMed

Casey, John P; Barbero, Roberto J; Heldman, Nimrod; Belcher, Angela M

2014-11-26

Biocatalysis has grown rapidly in recent decades as a solution to the evolving demands of industrial chemical processes. Mounting environmental pressures and shifting supply chains underscore the need for novel chemical activities, while rapid biotechnological progress has greatly increased the utility of enzymatic methods. Enzymes, though capable of high catalytic efficiency and remarkable reaction selectivity, still suffer from relative instability, high costs of scaling, and functional inflexibility. Herein, we developed a biochemical platform for engineering de novo semisynthetic enzymes, functionally modular and widely stable, based on the M13 bacteriophage. The hydrolytic bacteriophage described in this paper catalyzes a range of carboxylic esters, is active from 25 to 80 °C, and demonstrates greater efficiency in DMSO than in water. The platform complements biocatalysts with characteristics of heterogeneous catalysis, yielding high-surface area, thermostable biochemical structures readily adaptable to reactions in myriad solvents. As the viral structure ensures semisynthetic enzymes remain linked to the genetic sequences responsible for catalysis, future work will tailor the biocatalysts to high-demand synthetic processes by evolving new activities, utilizing high-throughput screening technology and harnessing M13's multifunctionality.

Permeability Measurements of Rock Samples from Conduit Drilling at Unzen Volcano, Japan

NASA Astrophysics Data System (ADS)

Watanabe, T.; Shimizu, Y.; Noguchi, S.; Nakada, S.

2006-12-01

The last eruption of Unzen Volcano (1990-1995) was effusive to form lava domes, though magmas at depths are estimated to have contained volatile materials enough to cause explosive eruptions [e.g., Sato et al., 1995]. Most of volatile materials should have escaped from ascending magmas. The escape of gas is controlled by permeability of magmas and country rocks. Unzen Scientific Drilling Project sampled both the latest conduit and its country rock (USDP-4). In order to understand degassing processes, we have measured the permeability of these rock samples. Four cube samples with edges of 25 mm were cut from USDP-4 cores C1, C12 (country rock), C13 and C14 (conduit). Sample C1 is considered as Old Unzen Lava, and Sample C12 volcanic breccia. The transient pulse method was employed to measure the permeability. It applies a step of the fluid pressure difference across a specimen, and measures the decay rate of the fluid pressure difference. This method can be applied to samples with very low permeability, since it determines the permeability without measuring the fluid flux. Nitrogen gas was used as a pore fluid. Our permeametry system is built in a pressure vessel, and the confining pressure and the pore fluid pressure can be controlled independently. The temperature of the measurement system is kept constant within 0.1 degree. The temperature control and the background leak rate limit the measurable permeability to be higher than 10^{-20} m2. Measurements were first conducted under the atmospheric pressure. The permeability in a rock sample varies with the direction by a factor less than 5. Sample C1 has the lowest permeability (10^{-19} m2), and Sample C12 the highest value (10^{-17 m2). The permeability of C13 and C14 is of the order of 10^{- 18} m2. Though only a trace of vesicles can be seen in conduit samples, the interconnection is still maintained. The pressure dependence of the permeability is now investigated up to 50 MPa. The permeability of C13 and C14 (conduit samples) decreases by around one order of magnitude as the confining pressure increases from the atmospheric pressure to 50 MPa. The pressure dependence sensitively reflects the geometry of pores that control the interconnection of pores. Implications for degassing processes will be discussed on the basis of measured permeability and SEM images.

Identification of a Peptide-Pheromone that Enhances Listeria monocytogenes Escape from Host Cell Vacuoles

PubMed Central

Xayarath, Bobbi; Alonzo, Francis; Freitag, Nancy E.

2015-01-01

Listeria monocytogenes is a Gram-positive facultative intracellular bacterial pathogen that invades mammalian cells and escapes from membrane-bound vacuoles to replicate within the host cell cytosol. Gene products required for intracellular bacterial growth and bacterial spread to adjacent cells are regulated by a transcriptional activator known as PrfA. PrfA becomes activated following L. monocytogenes entry into host cells, however the signal that stimulates PrfA activation has not yet been defined. Here we provide evidence for L. monocytogenes secretion of a small peptide pheromone, pPplA, which enhances the escape of L. monocytogenes from host cell vacuoles and may facilitate PrfA activation. The pPplA pheromone is generated via the proteolytic processing of the PplA lipoprotein secretion signal peptide. While the PplA lipoprotein is dispensable for pathogenesis, bacteria lacking the pPplA pheromone are significantly attenuated for virulence in mice and have a reduced efficiency of bacterial escape from the vacuoles of nonprofessional phagocytic cells. Mutational activation of PrfA restores virulence and eliminates the need for pPplA-dependent signaling. Experimental evidence suggests that the pPplA peptide may help signal to L. monocytogenes its presence within the confines of the host cell vacuole, stimulating the expression of gene products that contribute to vacuole escape and facilitating PrfA activation to promote bacterial growth within the cytosol. PMID:25822753

76 FR 31642 - Committee on Equal Opportunities in Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-01

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering; Notice of... Engineering (1173). Dates/Time: June 13, 2011, 9 a.m.-4:30 p.m. June 14, 2011, 9 a.m.-2 p.m. Places: June 13... participation in science and engineering. Agenda Monday, June 13, 2011 Opening Statement by the CEOSE Chair...

Knowledge-Based Integrated Information Systems Engineering: Highlights and Bibliography. Volume 1.

DTIC Science & Technology

1987-12-01

of database technology, 0 communication technology and expert systems technology. , Organizational issues cover the process of making controlled... process of linking strategic goals, technical issues , and organizational aspects can be depicted as shown in Figure 2.2. At the top level, strategic...an integrated information system design and implementation in a short period of time [4]. 2.2.2 Emphasis on Process It was mentioned in Section 1.3

13. INTERIOR OF ENGINE ROOM, CONTAINING MESTACORLISS CROSSCOMPOUND ENGINE, FOR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. INTERIOR OF ENGINE ROOM, CONTAINING MESTA-CORLISS CROSS-COMPOUND ENGINE, FOR 40" BLOOMING MILL. THIS VIEW HIGHLIGHTS THE CRANK AND 24' DIAMETER FLYWHEEL. - Republic Iron & Steel Company, Youngstown Works, Blooming Mill & Blooming Mill Engines, North of Poland Avenue, Youngstown, Mahoning County, OH

Generation of airborne listeria from floor drain

USDA-ARS?s Scientific Manuscript database

Listeria monocytogenes can colonize in floor drains in poultry processing plants and further throughout processing facilities, remaining present even after cleaning and disinfection of the plant. Therefore, during wash down, workers exercise caution to prevent escape and transfer of drain microflor...

The Lyman continuum escape fraction of low mass star-forming galaxies at z~1.

NASA Astrophysics Data System (ADS)

Rutkowski, Michael J.; Scarlata, Claudia; Haardt, Francesco; Siana, Brian D.; Rafelski, Marc; Henry, Alaina L.; Hayes, Matthew; Salvato, Mara; Pahl, Anthony; Mehta, Vihang; Beck, Melanie; Malkan, Matthew Arnold; Teplitz, Harry I.

2016-01-01

Star-forming galaxies (SFGs) in the high redshift universe (z>6) are believed to ionize neutral hydrogen in the intergalactic medium during the epoch of reionization. We tested this assumption by studying likely analogs of these SFGs in archival HST grism spectroscopy with GALEX UV and ground-based optical images at the redshift range in which we can directly measure the rest-frame Lyman continuum (λ<912Å, LyC) emission. We selected ~1400 SFGs for study on the presence of strong Hα emission and strongly selected against those SFGs whose GALEX FUV photometry could be contaminated by low redshift interlopers along the line of sight to produce a sample of ~600 z~1 SFGs. We made no unambiguous detection of escaping Lyman continuum radiation in individual galaxies in this sample, and stacked the individual non-detections in order to constrain the absolute Lyman continuum escape fraction, fesc<2% (3σ). We sub-divided this sample and stacked SFGs to measure upper limits to fesc with respect to stellar mass,luminosity and relative orientation. For z~1 high Hα equivalent width (EW>200Å) SFGs, we found for the first time an upper limit to fesc<9%. We discuss the implications of these limits for the ionizing emissivity of high redshift SFGs during the epoch of reionization. We conclude that reionization by SFGs is only marginally consistent with independent Planck observations of the CMB electron scattering opacity unless the LyC escape fraction of SFGs increases with redshift and an unobserved population of faint (MUV<-13 AB) SFGs contributes significantly to the UV background.

Antidepressant-like effects of alnespirone (S 20499) in the learned helplessness test in rats.

PubMed

Mac Sweeney, C P; Lesourd, M; Gandon, J M

1998-03-19

The effects of the new chroman derivative, alnespirone (S 20499), which is a selective 5-HT1A receptor agonist, were investigated in an animal model of depression, the learned helplessness test. Rats previously submitted to a session of 60 inescapable electric foot shocks (learned helpless controls) exhibited a deficit in escape performance in three subsequent shuttle-box sessions. Alnespirone was administered twice daily via the oral route (2.5, 5, 10, 20 mg kg(-1) day(-1)). It was shown to protect against the elevation in escape failures caused by exposure to the uncontrollable aversive situation at 5 and 10 mg kg(-1) day(-1) p.o. (13+/-2 and 10+/-3 escape failures, respectively, vs. 9+/-2 escape failures in control rats). In addition, alnespirone had a tendency to elevate the number of intertrial crossings during the resting periods, depending on the dose and day on which the avoidance task was performed (15+/-2 intertrial crossings at the dose of 5 mg kg(-1) day(-1), vs. 5+/-2 intertrial crossings for the helpless control rats, on the second day). In comparison, imipramine (64 mg kg(-1) day(-1) p.o.) provided marked protection on all three days of the avoidance task and tended to increase the number of intertrial crossings during the resting periods on the second and the third days. It is concluded that alnespirone exerts antidepressant-like properties in the learned helplessness test in rats, in a manner similar to 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)tetralin), buspirone and ipsapirone, other 5-HT1A receptor agonists.

Geophysical and atmospheric evolution of habitable planets.

PubMed

Lammer, Helmut; Selsis, Frank; Chassefière, Eric; Breuer, Doris; Griessmeier, Jean-Mathias; Kulikov, Yuri N; Erkaev, Nikolai V; Khodachenko, Maxim L; Biernat, Helfried K; Leblanc, Francois; Kallio, Esa; Lundin, Richard; Westall, Frances; Bauer, Siegfried J; Beichman, Charles; Danchi, William; Eiroa, Carlos; Fridlund, Malcolm; Gröller, Hannes; Hanslmeier, Arnold; Hausleitner, Walter; Henning, Thomas; Herbst, Tom; Kaltenegger, Lisa; Léger, Alain; Leitzinger, Martin; Lichtenegger, Herbert I M; Liseau, René; Lunine, Jonathan; Motschmann, Uwe; Odert, Petra; Paresce, Francesco; Parnell, John; Penny, Alan; Quirrenbach, Andreas; Rauer, Heike; Röttgering, Huub; Schneider, Jean; Spohn, Tilman; Stadelmann, Anja; Stangl, Günter; Stam, Daphne; Tinetti, Giovanna; White, Glenn J

2010-01-01

The evolution of Earth-like habitable planets is a complex process that depends on the geodynamical and geophysical environments. In particular, it is necessary that plate tectonics remain active over billions of years. These geophysically active environments are strongly coupled to a planet's host star parameters, such as mass, luminosity and activity, orbit location of the habitable zone, and the planet's initial water inventory. Depending on the host star's radiation and particle flux evolution, the composition in the thermosphere, and the availability of an active magnetic dynamo, the atmospheres of Earth-like planets within their habitable zones are differently affected due to thermal and nonthermal escape processes. For some planets, strong atmospheric escape could even effect the stability of the atmosphere.

Inefficient volatile loss from the Moon-forming disk: Reconciling the giant impact hypothesis and a wet Moon

NASA Astrophysics Data System (ADS)

Nakajima, Miki; Stevenson, David J.

2018-04-01

The Earth's Moon is thought to have formed from a circumterrestrial disk generated by a giant impact between the proto-Earth and an impactor approximately 4.5 billion years ago. Since this impact was energetic, the disk would have been hot (4000-6000 K) and partially vaporized (20-100% by mass). This formation process is thought to be responsible for the geochemical observation that the Moon is depleted in volatiles (e.g., K and Na). To explain this volatile depletion, some studies suggest the Moon-forming disk was rich in hydrogen, which was dissociated from water, and it escaped from the disk as a hydrodynamic wind accompanying heavier volatiles (hydrodynamic escape). This model predicts that the Moon should be significantly depleted in water, but this appears to contradict some of the recently measured lunar water abundances and D/H ratios that suggest that the Moon is more water-rich than previously thought. Alternatively, the Moon could have retained its water if the upper parts (low pressure regions) of the disk were dominated by heavier species because hydrogen would have had to diffuse out from the heavy-element rich disk, and therefore the escape rate would have been limited by this slow diffusion process (diffusion-limited escape). To identify which escape the disk would have experienced and to quantify volatiles loss from the disk, we compute the thermal structure of the Moon-forming disk considering various bulk water abundances (100-1000 ppm) and mid-plane disk temperatures (2500-4000 K). Assuming that the disk consists of silicate (SiO2 or Mg2SiO4) and water and that the disk is in the chemical equilibrium, our calculations show that the upper parts of the Moon-forming disk are dominated by heavy atoms or molecules (SiO and O at Tmid > 2500- 2800 K and H2O at Tmid < 2500- 2800 K) and hydrogen is a minor species. This indicates that hydrogen escape would have been diffusion-limited, and therefore the amount of lost water and hydrogen would have been small compared to the initial abundance assumed. This result indicates that the giant impact hypothesis can be consistent with the water-rich Moon. Furthermore, since the hydrogen wind would have been weak, the other volatiles would not have escaped either. Thus, the observed volatile depletion of the Moon requires another mechanism.

33 CFR 211.13 - Approval of the Office of the Chief of Engineers.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Approval of the Office of the Chief of Engineers. 211.13 Section 211.13 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE REAL ESTATE ACTIVITIES OF THE CORPS OF ENGINEERS IN CONNECTION WITH CIVIL WORKS PROJECTS Disposal of Real Estate...

Vapor crystal growth technology development: Application to cadmium telluride

NASA Technical Reports Server (NTRS)

Rosenberger, Franz; Banish, Michael; Duval, Walter M. B.

1991-01-01

Growth of bulk crystals by physical vapor transport was developed and applied to cadmium telluride. The technology makes use of effusive ampoules, in which part of the vapor contents escapes to a vacuum shroud through defined leaks during the growth process. This approach has the advantage over traditional sealed ampoule techniques that impurity vapors and excess vapor constituents are continuously removed from the vicinity of the growing crystal. Thus, growth rates are obtained routinely at magnitudes that are rather difficult to achieve in closed ampoules. Other advantages of this effusive ampoule physical vapor transport (EAPVT) technique include the predetermination of transport rates based on simple fluid dynamics and engineering considerations, and the growth of the crystal from close to congruent vapors, which largely alleviates the compositional nonuniformities resulting from buoyancy driven convective transport. After concisely reviewing earlier work on improving transport rates, nucleation control, and minimization of crystal wall interactions in vapor crystal growth, a detail account is given of the largely computer controlled EAPVT experimentation.

Verge and Foliot Clock Escapement: A Simple Dynamical System

ERIC Educational Resources Information Center

Denny, Mark

2010-01-01

The earliest mechanical clocks appeared in Europe in the 13th century. From about 1250 CE to 1670 CE, these simple clocks consisted of a weight suspended from a rope or chain that was wrapped around a horizontal axle. To tell time, the weight must fall with a slow uniform speed, but, under the action of gravity alone, such a suspended weight would…

46 CFR 113.35-13 - Mechanical engine order telegraph systems; operation.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Mechanical engine order telegraph systems; operation...) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-13 Mechanical engine order telegraph systems; operation. If more than one transmitter operates a...

46 CFR 113.35-13 - Mechanical engine order telegraph systems; operation.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Mechanical engine order telegraph systems; operation...) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-13 Mechanical engine order telegraph systems; operation. If more than one transmitter operates a...

46 CFR 113.35-13 - Mechanical engine order telegraph systems; operation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Mechanical engine order telegraph systems; operation...) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-13 Mechanical engine order telegraph systems; operation. If more than one transmitter operates a...

46 CFR 113.35-13 - Mechanical engine order telegraph systems; operation.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Mechanical engine order telegraph systems; operation...) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-13 Mechanical engine order telegraph systems; operation. If more than one transmitter operates a...

Biomechanics and Thermodynamics of Nanoparticle Interactions with Plasma and Endosomal Membrane Lipids in Cellular Uptake and Endosomal Escape

PubMed Central

2015-01-01

To be effective for cytoplasmic delivery of therapeutics, nanoparticles (NPs) taken up via endocytic pathways must efficiently transport across the cell membrane and subsequently escape from the secondary endosomes. We hypothesized that the biomechanical and thermodynamic interactions of NPs with plasma and endosomal membrane lipids are involved in these processes. Using model plasma and endosomal lipid membranes, we compared the interactions of cationic NPs composed of poly(d,l-lactide-co-glycolide) modified with the dichain surfactant didodecyldimethylammonium bromide (DMAB) or the single-chain surfactant cetyltrimethylammonium bromide (CTAB) vs anionic unmodified NPs of similar size. We validated our hypothesis in doxorubicin-sensitive (MCF-7, with relatively fluid membranes) and resistant breast cancer cells (MCF-7/ADR, with rigid membranes). Despite their cationic surface charges, DMAB- and CTAB-modified NPs showed different patterns of biophysical interaction: DMAB-modified NPs induced bending of the model plasma membrane, whereas CTAB-modified NPs condensed the membrane, thereby resisted bending. Unmodified NPs showed no effects on bending. DMAB-modified NPs also induced thermodynamic instability of the model endosomal membrane, whereas CTAB-modified and unmodified NPs had no effect. Since bending of the plasma membrane and destabilization of the endosomal membrane are critical biophysical processes in NP cellular uptake and endosomal escape, respectively, we tested these NPs for cellular uptake and drug efficacy. Confocal imaging showed that in both sensitive and resistant cells DMAB-modified NPs exhibited greater cellular uptake and escape from endosomes than CTAB-modified or unmodified NPs. Further, paclitaxel-loaded DMAB-modified NPs induced greater cytotoxicity even in resistant cells than CTAB-modified or unmodified NPs or drug in solution, demonstrating the potential of DMAB-modified NPs to overcome the transport barrier in resistant cells. In conclusion, biomechanical interactions with membrane lipids are involved in cellular uptake and endosomal escape of NPs. Biophysical interaction studies could help us better understand the role of membrane lipids in cellular uptake and intracellular trafficking of NPs. PMID:24911361

Inferring Fish Escape Behaviour in Trawls Based on Catch Comparison Data: Model Development and Evaluation Based on Data from Skagerrak, Denmark

PubMed Central

Karlsen, Junita Diana

2014-01-01

During the fishing process, fish react to a trawl with a series of behaviours that often are species and size specific. Thus, a thorough understanding of fish behaviour in relation to fishing gear and a scientific understanding of the ability of different gear designs to utilize or stimulate various behavioural patterns during the catching process are essential for developing more efficient, selective, and environmentally friendly trawls. Although many behavioural studies using optical and acoustic observation systems have been conducted, harsh observation conditions on the fishing grounds often hamper the ability to directly observe fish behaviour in relation to fishing gear. As an alternative to optical and acoustic methods, we developed and applied a new mathematical model to catch data to extract detailed and quantitative information about species- and size-dependent escape behaviour in towed fishing gear such as trawls. We used catch comparison data collected with a twin trawl setup; the only difference between the two trawls was that a 12 m long upper section was replaced with 800 mm diamond meshes in one of them. We investigated the length-based escape behaviour of cod (Gadus morhua), haddock (Melanogrammus aeglefinus), saithe (Pollachius virens), witch flounder (Glyptocephalus cynoglossus), and lemon sole (Microstomus kitt) and quantified the extent to which behavioural responses set limits for the large mesh panel’s selective efficiency. Around 85% of saithe, 80% of haddock, 44% of witch flounder, 55% of lemon sole, and 55% of cod (below 68 cm) contacted the large mesh panel and escaped. We also demonstrated the need to account for potential selectivity in the trawl body, as it can bias the assessment of length-based escape behaviour. Our indirect assessment of fish behaviour was in agreement with the direct observations made for the same species in a similar section of the trawl body reported in the literature. PMID:24586403

POSTTRAUMATIC STRESS DISORDER AMONG INDONESIAN CHILDREN 5 YEARS AFTER THE TSUNAMI.

PubMed

Irwanto; Faisal; Zulfa, Hendra

2015-09-01

Children are at risk for developing posttraumatic stress disorder (PTSD) due to experiencing or living in a disaster area. The factors that increase the likelihood of a child developing PTSD need further clarification. We studied the factors associated with PTSD among children who experienced the tsunami in Sumatra, Indonesia. We conducted a cross sectional study in 2 subdistricts of Sumatra 5 years after experiencing a tsunami. Children aged 7-13 years were enrolled using stratified cluster sampling. A tsunami-modified version of The PsySTART Rapid Triage System was used to question children about their tsunami-specific traumatic experiences. Trauma symptoms were evaluated using the Trauma Symptom Checklist For Children (TSCC). The diagnosis of PTSD was made using the Child PTSD Symptom Scale (CPSS) and DSM-IV criteria. The data were analyzed with chi-square tests and multivariate logistic regression analysis with 95% confidence intervals (CI). A total of 262 children were enrolled in this study. The prevalence of PTSD in these children was 20.6%. On multivariate analysis, having experienced a delay in evacuation (PR = 4.5; 95% CI: 2.794-13.80; p < 0.001) and being unable to escape (PR = 13.07; 95% CI: 5.884-64; p < 0.001) were significantly associated with PTSD 5 years after the tsunami. Children who experienced a traumatic event in which they were unable to escape or when there is a delay in evacuation are at risk of developing PTSD and need appropriate treatment.

IMEC-9: The 9th Israel Materials Engineering Conference. Program & Abstracts

DTIC Science & Technology

1999-12-07

non- toxic , magnetite (FesC^) nanoparticles of very narrow size distribution in sizes ranging from approximately 20 nm up to 0.1 urn. The process for...Israel 17 Composites I Hall G Chair: R. Albalak 13:30 -13:50 Synthesis of Dense Oxide -Based In Situ Composites via Thermal Explosion/SHS...Tsionsky, Israel 16:00 -16:20 The Effect of Composition and Microstructure on the Corrosion Behavior of Magnesium- Aluminium Alloys P. Uzan, D. Eliezer

NASA/DOD Aerospace Knowledge Diffusion Research Project. Paper 13: The information-seeking habits and practices of engineers

NASA Technical Reports Server (NTRS)

Pinelli, Thomas E.

1991-01-01

It is argued that only by maximizing the research and development process can the United States maintain and possibly capture its international competitive edge. Key to this goal is the provision of information services and products which meet the information needs of engineers. Evidence exists which indicates that traditional information services and products may, in fact, not be meeting the information needs of engineers. The primary reason for this deficiency is three fold. First, the specific information needs of engineers are neither well known nor well understood. Second, what is known about the information seeking habits and practices of engineers has not been applied to existing engineering information services. Third, the information professionals continue to over-emphasize technology instead of concentrating on the quality of the information itself and the ability of the information to meet the needs of the user.

Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials.

PubMed

Jin, Hyo-Eon; Lee, Seung-Wuk

2018-01-01

M13 bacteriophages have several qualities that make them attractive candidates as building blocks for tissue regenerating scaffold materials. Through genetic engineering, a high density of functional peptides and proteins can be simultaneously displayed on the M13 bacteriophage's outer coat proteins. The resulting phage can self-assemble into nanofibrous network structures and can guide the tissue morphogenesis through proliferation, differentiation and apoptosis. In this manuscript, we will describe methods to develop major coat-engineered M13 phages as a basic building block and aligned tissue-like matrices to develop regenerative nanomaterials.

Metabolic engineering for the production of 1,3-propanediol

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cameron, D.C.; Tong, I.T., Skraly, F.A.

1993-12-31

Metabolic engineering involves the use of recombinant DNA techniques for the modification of intermediary metabolic pathways. Microorganisms have recently been engineered to produce compounds such as indigo, ethanol, fatty acids and polyhydroxyalkanoates. As a model system for research in metabolic engineering, the authors have constructed a strain of the bacterium Escherichia coli, that is able to produce 1,3-propanediol (1,3-PD) from glycerol. This strain contains the genes for the glycerol deydratase and the 1,3-PD oxidoreductase from Klebsiella pneumoniae. The authors have also investigated genetic and environmental strategies for improving the yield and productivity of 1,3-PD by the engineered organism. In additionmore » to being a useful model system, 1,3-PD production is of current practical interest. First 1,3-PD (also known as trimethylene glycol) and 1,4-butanediol, the more readily available diols. Second, the volume of feedstock (glycerol) is expected to grow, as it is a by-product of the production of polyglycoside surfactants and biodiesel fluids.« less

Succession of hide–seek and pursuit–evasion at heterogeneous locations

PubMed Central

Gal, Shmuel; Casas, Jérôme

2014-01-01

Many interactions between searching agents and their elusive targets are composed of a succession of steps, whether in the context of immune systems, predation or counterterrorism. In the simplest case, a two-step process starts with a search-and-hide phase, also called a hide-and-seek phase, followed by a round of pursuit–escape. Our aim is to link these two processes, usually analysed separately and with different models, in a single game theory context. We define a matrix game in which a searcher looks at a fixed number of discrete locations only once each searching for a hider, which can escape with varying probabilities according to its location. The value of the game is the overall probability of capture after k looks. The optimal search and hide strategies are described. If a searcher looks only once into any of the locations, an optimal hider chooses it's hiding place so as to make all locations equally attractive. This optimal strategy remains true as long as the number of looks is below an easily calculated threshold; however, above this threshold, the optimal position for the hider is where it has the highest probability of escaping once spotted. PMID:24621817

A dual-mode secure UHF RFID tag with a crypto engine in 0.13-μm CMOS

NASA Astrophysics Data System (ADS)

Tao, Yang; Linghao, Zhu; Xi, Tan; Junyu, Wang; Lirong, Zheng; Hao, Min

2016-07-01

An ultra-high-frequency (UHF) radio frequency identification (RFID) secure tag chip with a non-crypto mode and a crypto mode is presented. During the supply chain management, the tag works in the non-crypto mode in which the on-chip crypto engine is not enabled and the tag chip has a sensitivity of -12.8 dBm for long range communication. At the point of sales (POS), the tag will be switched to the crypto mode in order to protect the privacy of customers. In the crypto mode, an advanced encryption standard (AES) crypto engine is enabled and the sensitivity of the tag chip is switched to +2 dBm for short range communication, which is a method of physical protection. The tag chip is implemented and verified in a standard 0.13-μm CMOS process. Project supported by the National Science & Technology Pillar Program of China (No. 2015BAK36B01).

Effect of water on hydrogen permeability. [Stirling engines

NASA Technical Reports Server (NTRS)

Hulligan, D. D.; Tomazic, W. A.

1984-01-01

Doping of hydrogen with CO or CO2 was developed to reduce hydrogen permeation in Stirling engines by forming low permeability oxide coatings in the heater tubes. An end product of this process is water - which can condense in the cold parts of the engine system. If the water vapor is reduced to a low enough level, the hydrogen can reduce the oxide coating resulting in increased permeability. The equilibrium level of water (oxygen bearing gas) required to avoid reduction of the oxide coating was investigated. Results at 720 C and 13.8 MPa have shown that: (1) pure hydrogen will reduce the coating; (2) 500 ppm CO (500 ppm water equivalent) does not prevent the reduction; and (3) 500 ppm CO2 (1000 ppm water) appears to be close to the equilibrium level. Further tests are planned to define the equilibrium level more precisely and to extend the data to 820 C and 3.4, 6.9, and 13.8 MPa.

Trapping of hydrogen atoms in X-irradiated salts at room temperature and the decay kinetics

NASA Technical Reports Server (NTRS)

May, C. E.; Philipp, W. H.; Marsik, S. J.

1974-01-01

The salts (hypophosphites, formates, a phosphite, a phosphate, and an oxalate) were X-irradiated, whereby hydrogen formed chemically by a radiolytic process becomes trapped in the solid. By room temperature vacuum extraction, the kinetics for the evolution of this trapped hydrogen was studied mass spectrometrically. All salts except two exhibited second-order kinetics. The two exceptions (NaH2PO2(H2O) and K2HPO4) showed first-order kinetics. Based on experimental results, the escape of hydrogen involves three steps: the diffusion of hydrogen atoms from the bulk to the surface, association of these atoms on the surface (rate controlling step for second-order hydrogen evolution), and the desorption of molecular hydrogen from the surface. The hydrogen does not escape if the irradiated salt is stored in air, apparently because adsorbed air molecules occupy surface sites required in the escape mechanism.

Mechanism for the acceleration and ejection of dust grains from Jupiter's magnetosphere

NASA Technical Reports Server (NTRS)

Horanyi, M.; Morfill, G.; Gruen, E.

1993-01-01

The Ulysses mission detected quasi-periodic streams of high-velocity submicron-sized dust particles during its encounter with Jupiter. It is shown here how the dust events could result from the acceleration and subsequent ejection of small grains by Jupiter's magnetosphere. Dust grains entering the plasma environment of the magnetosphere become charged, with the result that their motion is then determined by both electromagnetic and gravitational forces. This process is modeled, and it is found that only those particles in a certain size range gain sufficient energy to escape the Jovian system. Moreover, if Io is assumed to be the source of the dust grains, its location in geographic and geomagnetic coordinates determines the exit direction of the escaping particles, providing a possible explanation for the observed periodicities. The calculated mass and velocity range of the escaping dust gains are consistent with the Ulysses findings.

Simulation of Locking Space Truss Deployments for a Large Deployable Sparse Aperture Reflector

DTIC Science & Technology

2015-03-01

Dr. Alan Jennings, for his unending patience with my struggles through this entire process . Without his expertise, guidance, and trust I would have...engineer since they are not automatically meshed. Fortunately, the mesh process is quite swift. Figure 13 shows both a linear hexahedral element as well...less than that of the serial process . Therefore, COMSOL’s partially parallelized algorithms will not be sped up as a function of cores added and is

Meteorite constraints on Martian atmospheric loss and paleoclimate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cassata, William S.

The evolution of Mars' atmosphere to its currently thin state incapable of supporting liquid water remains poorly understood and has important implications for Martian climate history. Martian meteorites contain trapped atmospheric gases that can be used to constrain both the timing and effectiveness of atmospheric escape processes. Here in this article, measurements of xenon isotopes in two ancient Martian meteorites, ALH 84001 and NWA 7034, are reported. The data indicate an early episode of atmospheric escape that mass fractionated xenon isotopes culminated within a few hundred million years of planetary formation, and little change to the atmospheric xenon isotopic compositionmore » has occurred since this time. In contrast, on Earth atmospheric xenon fractionation continued for at least two billion years (Pujol et al., 2011). Such differences in atmospheric Xe fractionation between the two planets suggest that climate conditions on Mars may have differed significantly from those on Archean Earth. For example, the hydrogen escape flux may not have exceeded the threshold required for xenon escape on Mars after 4.2–4.3 Ga, which indicates that Mars may have been significantly drier than Earth after this time.« less

Ionosphere-Magnetosphere Coupling from Dynamics Explorer to Swarm and Beyond

NASA Astrophysics Data System (ADS)

Moore, T. E.; Clemmons, J. H.; Collinson, G.; Gershman, D. J.; Khazanov, G. V.; Kistler, L. M.; Knudsen, D. J.; Pfaff, R. F., Jr.; Pollock, C. J.; Rowland, D. E.

2014-12-01

Ionospheric plasmas have thermal speeds much smaller than orbital (escape) velocities, so they appear to orbiting spacecraft as a relatively narrow beam from the ram direction. But one of the very interesting things about ionospheric plasmas is that they are heated strongly in the auroral zones, and accelerated such that their thermal or directed speeds exceed orbital or escape velocity. Then they do just that: orbit or escape the Earth into the magnetosphere and beyond at velocities spread over a large range of angles and magnitudes. This has created a dichotomy between the observation of cold, rammed plasmas in the ionosphere proper and the observation of hot plasmas with a large range of velocities in both direction and magnitude, well above the ionosphere proper. The former are usually observed from attitude-stabilized, ram-pointed spacecraft, while the latter are usually observed from spinning spacecraft. We review measurements that illuminate the way forward, particularly from the Swarm mission, and propose a new sciencecraft configuration that overcomes the divergent requirements to facilitate direct observations of processes that heat and accelerate ionospheric plasmas across this transition from gravitationally bound to unbound and escaping into space.

Meteorite constraints on Martian atmospheric loss and paleoclimate

DOE PAGES

Cassata, William S.

2017-10-06

The evolution of Mars' atmosphere to its currently thin state incapable of supporting liquid water remains poorly understood and has important implications for Martian climate history. Martian meteorites contain trapped atmospheric gases that can be used to constrain both the timing and effectiveness of atmospheric escape processes. Here in this article, measurements of xenon isotopes in two ancient Martian meteorites, ALH 84001 and NWA 7034, are reported. The data indicate an early episode of atmospheric escape that mass fractionated xenon isotopes culminated within a few hundred million years of planetary formation, and little change to the atmospheric xenon isotopic compositionmore » has occurred since this time. In contrast, on Earth atmospheric xenon fractionation continued for at least two billion years (Pujol et al., 2011). Such differences in atmospheric Xe fractionation between the two planets suggest that climate conditions on Mars may have differed significantly from those on Archean Earth. For example, the hydrogen escape flux may not have exceeded the threshold required for xenon escape on Mars after 4.2–4.3 Ga, which indicates that Mars may have been significantly drier than Earth after this time.« less

Constraints on Mars Hydrogen loss from MAVEN: processes and present-day rates

NASA Astrophysics Data System (ADS)

Chaffin, M.; Deighan, J.; Stewart, I. F.; Schneider, N. M.; Chaufray, J. Y.; Jain, S.; Thiemann, E.; Mayyasi, M.; Clarke, J. T.; Crismani, M. M. J.; Stiepen, A.; Montmessin, F.; Epavier, F.; McClintock, B.; Holsclaw, G.; Jakosky, B. M.

2017-12-01

The surface of Mars today is dessicated and oxidized, despite a large body of evidence indicating that the planet was wet and redox neutral early in its history. H escape has the potential to explain both conditions, but until recently there was no long-term monitoring of H loss at Mars. The presence of MAVEN at Mars since late 2014 has established a seasonal record of H escape via airglow measurements of coronal hydrogen and the flux of energetic particles whose production is mediated by the H corona. H escape appears from multiple proxies to be more than an order of magnitude larger in Southern summer than Northern summer, potentially as a consequence of enhanced water transport to the middle atmosphere. This newly described escape channel potentially dominates water loss from Mars today and over its history, and may be the most important control on the chemistry of the atmosphere and surface. I will present an overview of MAVEN measurements of H loss, focusing on contributions made by the Imaging Ultraviolet Spectrograph, and discuss how photochemical models of the atmosphere may need to be adjusted to incorporate new mechanisms for H loss.

Investigating the Lyman photon escape in local starburst galaxies with the Cosmic Origins Spectrograph ★

NASA Astrophysics Data System (ADS)

Hernandez, Svea; Leitherer, Claus; Boquien, Médéric; Buat, Véronique; Burgarella, Denis; Calzetti, Daniela; Noll, Stefan

2018-04-01

We present a study of 7 star-forming galaxies from the Cosmic Evolution Survey (COSMOS) observed with the Cosmic Origins Spectrograph (COS) on board the Hubble Space Telescope (HST). The galaxies are located at relatively low redshifts, z ˜0.3, with morphologies ranging from extended and disturbed to compact and smooth. To complement the HST observations we also analyze observations taken with the VIMOS spectrograph on the Very Large Telescope (VLT). In our galaxy sample we identify three objects with double peak Lyman-α profiles similar to those seen in Green Pea compact galaxies and measure peak separations of 655, 374, and 275 km s-1. We measure Lyman-α escape fractions with values ranging between 5-13%. Given the low flux levels in the individual COS exposures we apply a weighted stacking approach to obtain a single spectrum. From this COS combined spectrum we infer upper limits for the absolute and relative Lyman continuum escape fractions of f_abs(LyC) = 0.4^{+10.1}_{-0.4}% and f_res(LyC) = 1.7^{+15.2}_{-1.7}%, respectively. Finally, we find that most of these galaxies have moderate UV and optical SFRs (SFRs ≲ 10 M⊙ yr-1).

Investigating the Lyman photon escape in local starburst galaxies with the Cosmic Origins Spectrograph

NASA Astrophysics Data System (ADS)

Hernandez, Svea; Leitherer, Claus; Boquien, Médéric; Buat, Véronique; Burgarella, Denis; Calzetti, Daniela; Noll, Stefan

2018-07-01

We present a study of seven star-forming galaxies from the Cosmic Evolution Survey observed with the Cosmic Origins Spectrograph (COS) onboard the Hubble Space Telescope (HST). The galaxies are located at relatively low redshifts, z ˜ 0.3, with morphologies ranging from extended and disturbed to compact and smooth. To complement the HST observations, we also analyse observations taken with the Visible Multi-object Spectrograph (VIMOS) on the Very Large Telescope (VLT). In our galaxy sample, we identify three objects with double peak Lyman-α profiles similar to those seen in Green Pea compact galaxies and measure peak separations of 655, 374, and 275 km s-1. We measure Lyman-α escape fractions with values ranging between 5 per cent and 13 per cent. Given the low flux levels in the individual COS exposures, we apply a weighted stacking approach to obtain a single spectrum. From this COS combined spectrum, we infer upper limits for the absolute and relative Lyman continuum escape fractions of f_abs(LyC) = 0.4^{+10.1}_{-0.4} per cent and f_res(LyC) = 1.7^{+15.2}_{-1.7}per cent, respectively. Finally, we find that most of these galaxies have moderate ultraviolet and optical star formation rates (SFRs) (SFRs ≲10 M⊙ yr-1).

High Mortality without ESCAPE: The Registry of Heart Failure Patients Receiving Pulmonary Artery Catheters without Randomization

PubMed Central

Allen, Larry A.; Rogers, Joseph G.; Warnica, J. Wayne; DiSalvo, Thomas G.; Tasissa, Gudaye; Binanay, Cynthia; O’Connor, Christopher M.; Califf, Robert M.; Leier, Carl V.; Shah, Monica R.; Stevenson, Lynne W.

2008-01-01

Background In ESCAPE, there was no difference in days alive and out of the hospital for patients with decompensated heart failure (HF) randomly assigned to therapy guided by pulmonary artery catheter (PAC) plus clinical assessment versus clinical assessment alone. The external validity of these findings is debated. Methods and Results ESCAPE sites enrolled 439 patients receiving PAC without randomization in a prospective registry. Baseline characteristics, pertinent trial exclusion criteria, reasons for PAC use, hemodynamics, and complications were collected. Survival was determined from the National Death Index and the Alberta Registry. On average, registry patients had lower blood pressure, worse renal function, less neurohormonal antagonist therapy, and higher use of intravenous inotropes as compared with trial patients. Although clinical assessment anticipated less volume overload and greater hypoperfusion among the registry population, measured filling pressures were similarly elevated in the registry and trial, while measured perfusion was slightly higher among registry patients. Registry patients had longer hospitalization (13 vs. 6 days, p <0.001) and higher 6-month mortality (34% vs. 20%, p < 0.001) than trial patients. Conclusions The decision to use PAC without randomization identified a population with higher disease severity and risk of mortality. This prospective registry highlights the complex context of patient selection for randomized trials. PMID:18926438

Hydrodynamical Modeling of Hydrogen Escape from Rocky Planets

NASA Astrophysics Data System (ADS)

Barringer, Daniel; Zugger, M.; Kasting, J.

2013-01-01

Hydrogen escape affects both the composition of primitive atmospheres of terrestrial planets and the planet’s state of oxidation. On Mars, hydrogen escape played a critical role in how long the planet remained in a warm wet state amenable to life. For both solar and extrasolar planets, hydrogen-rich atmospheres are better candidates for originating life by way of Miller-Urey-type prebiotic synthesis. However, calculating the rate of atmospheric hydrogen escape is difficult, for a number of reasons. First, the escape can be controlled either by diffusion through the homopause or by conditions in the upper atmosphere, whichever is slower. Second, both thermal and non-thermal escape mechanisms are typically important. Third, thermal escape itself can be subdivided into Jeans escape (thin upper atmosphere), and hydrodynamic escape, and hydrodynamic escape can be further subdivided into transonic escape and slower subsonic escape, depending on whether the exobase occurs above or below the sonic point. Additionally, the rate of escape for real terrestrial planet atmospheres, which are not 100% hydrogen, depends upon the concentration of infrared coolants, and upon heating and photochemistry driven largely by extreme ultraviolet (EUV) radiation. We have modified an existing 1-D model of hydrodynamic escape (F. Tian et al., JGR, 2008) to work in the high- hydrogen regime. Calculations are underway to determine hydrogen escape rates as a function of atmospheric H2 mixing ratio and the solar EUV flux. We will compare these rates with the estimated upper limit on the escape rate based on diffusion. Initial results for early Earth and Mars will later be extended to rocky exoplanets.

The Chemical Adventures of Sherlock Holmes: The Blackwater Escape.

ERIC Educational Resources Information Center

Waddell, Thomas G.; Rybolt, Thomas R.

2003-01-01

Presents a mystery based on the well-known characters, Sherlock Holmes and Dr. Watson. Emphasizes qualitative inorganic analysis, laboratory observations, and oxidation-reduction processes. (Author/YDS)

Time Intervals in Sequence Sampling, Not Data Modifications, Have a Major Impact on Estimates of HIV Escape Rates

PubMed Central

2018-01-01

The ability of human immunodeficiency virus (HIV) to avoid recognition by humoral and cellular immunity (viral escape) is well-documented, but the strength of the immune response needed to cause such a viral escape remains poorly quantified. Several previous studies observed a more rapid escape of HIV from CD8 T cell responses in the acute phase of infection compared to chronic infection. The rate of HIV escape was estimated with the help of simple mathematical models, and results were interpreted to suggest that CD8 T cell responses causing escape in acute HIV infection may be more efficient at killing virus-infected cells than responses that cause escape in chronic infection, or alternatively, that early escapes occur in epitopes mutations in which there is minimal fitness cost to the virus. However, these conclusions were challenged on several grounds, including linkage and interference of multiple escape mutations due to a low population size and because of potential issues associated with modifying the data to estimate escape rates. Here we use a sampling method which does not require data modification to show that previous results on the decline of the viral escape rate with time since infection remain unchanged. However, using this method we also show that estimates of the escape rate are highly sensitive to the time interval between measurements, with longer intervals biasing estimates of the escape rate downwards. Our results thus suggest that data modifications for early and late escapes were not the primary reason for the observed decline in the escape rate with time since infection. However, longer sampling periods for escapes in chronic infection strongly influence estimates of the escape rate. More frequent sampling of viral sequences in chronic infection may improve our understanding of factors influencing the rate of HIV escape from CD8 T cell responses. PMID:29495443

Time Intervals in Sequence Sampling, Not Data Modifications, Have a Major Impact on Estimates of HIV Escape Rates.

PubMed

Ganusov, Vitaly V

2018-02-27

The ability of human immunodeficiency virus (HIV) to avoid recognition by humoral and cellular immunity (viral escape) is well-documented, but the strength of the immune response needed to cause such a viral escape remains poorly quantified. Several previous studies observed a more rapid escape of HIV from CD8 T cell responses in the acute phase of infection compared to chronic infection. The rate of HIV escape was estimated with the help of simple mathematical models, and results were interpreted to suggest that CD8 T cell responses causing escape in acute HIV infection may be more efficient at killing virus-infected cells than responses that cause escape in chronic infection, or alternatively, that early escapes occur in epitopes mutations in which there is minimal fitness cost to the virus. However, these conclusions were challenged on several grounds, including linkage and interference of multiple escape mutations due to a low population size and because of potential issues associated with modifying the data to estimate escape rates. Here we use a sampling method which does not require data modification to show that previous results on the decline of the viral escape rate with time since infection remain unchanged. However, using this method we also show that estimates of the escape rate are highly sensitive to the time interval between measurements, with longer intervals biasing estimates of the escape rate downwards. Our results thus suggest that data modifications for early and late escapes were not the primary reason for the observed decline in the escape rate with time since infection. However, longer sampling periods for escapes in chronic infection strongly influence estimates of the escape rate. More frequent sampling of viral sequences in chronic infection may improve our understanding of factors influencing the rate of HIV escape from CD8 T cell responses.

Underwater detectibility of a lighting system on a helicopter escape exit.

PubMed

O'Neill, Brendan D; Kozey, John W; Brooks, Chris J

2004-06-01

When a helicopter ditches into water, it immediately inverts due to the weight of the engines and then fills with water. Locating the emergency exit for escape under such conditions is a difficult task. A new lighting system for an escape exit has been developed that illuminates on contact with water. The detectibility of the lighting was investigated under varying conditions of ambient illumination, water turbidity, and viewing distance. A total of 288 underwater detection trials were carried out by 9 subjects with an illuminated hatch placed at 2 distances (1.5 m and 3.1 m), under 2 ambient illuminations (bright: > 3000 lux and dark: < 0.1 lux), and in 2 conditions of water turbidity. The water temperature was 12 degrees C for all conditions. At 1.5 m, the lighting system was detectable in less than 1.5 s by all subjects in both clear and turbid water and under both bright and dark conditions. At 3.1 m, the lights were detectable in both clear and turbid water under the dark condition and in clear water under the bright condition. However, the lighting was not reliably detected in turbid water under bright condition. The system met original design requirements in terms of detectibility at 1.5 m. The detection time was always under 1.5 s. It could also be detected at 3.1 m in clear and turbid water, under dark conditions. However, the detectibility at 3.1 m in turbid water, under bright condition was less reliable.

Inhibition of chaotic escape from a potential well by incommensurate escape-suppressing excitations.

PubMed

Chacón, R; Martínez, J A

2002-03-01

Theoretical results are presented concerning the reduction of chaotic escape from a potential well by means of a harmonic parametric excitation that satisfies an ultrasubharmonic resonance condition with the escape-inducing excitation. The possibility of incommensurate escape-suppressing excitations is demonstrated by studying rational approximations to the irrational escape-suppressing frequency. The analytical predictions for the suitable amplitudes and initial phases of the escape-suppressing excitation are tested against numerical simulations based on a high-resolution grid of initial conditions. These numerical results indicate that the reduction of escape is reliably achieved for small amplitudes and at, and only at, the predicted initial phases. For the case of irrational escape-suppressing frequencies, the effective escape-reducing initial phases are found to lie close to the accumulation points of the set of suitable initial phases that are associated with the complete series of convergents up to the convergent giving the chosen rational approximation.

14 CFR 13.18 - Civil penalties: Administrative assessment against an individual acting as a pilot, flight...

Code of Federal Regulations, 2013 CFR

2013-01-01

... against an individual acting as a pilot, flight engineer, mechanic, or repairman. 13.18 Section 13.18... assessment against an individual acting as a pilot, flight engineer, mechanic, or repairman. (a) General. (1... procedures against an individual acting as a pilot, flight engineer, mechanic, or repairman, under 49 U.S.C...

Synergizing 13C Metabolic Flux Analysis and Metabolic Engineering for Biochemical Production.

PubMed

Guo, Weihua; Sheng, Jiayuan; Feng, Xueyang

Metabolic engineering of industrial microorganisms to produce chemicals, fuels, and drugs has attracted increasing interest as it provides an environment-friendly and renewable route that does not depend on depleting petroleum sources. However, the microbial metabolism is so complex that metabolic engineering efforts often have difficulty in achieving a satisfactory yield, titer, or productivity of the target chemical. To overcome this challenge, 13 C Metabolic Flux Analysis ( 13 C-MFA) has been developed to investigate rigorously the cell metabolism and quantify the carbon flux distribution in central metabolic pathways. In the past decade, 13 C-MFA has been widely used in academic labs and the biotechnology industry to pinpoint the key issues related to microbial-based chemical production and to guide the development of the appropriate metabolic engineering strategies for improving the biochemical production. In this chapter we introduce the basics of 13 C-MFA and illustrate how 13 C-MFA has been applied to synergize with metabolic engineering to identify and tackle the rate-limiting steps in biochemical production.

Demographic processes affect HIV-1 evolution in primary infection before the onset of selective processes.

PubMed

Herbeck, Joshua T; Rolland, Morgane; Liu, Yi; McLaughlin, Sherry; McNevin, John; Zhao, Hong; Wong, Kim; Stoddard, Julia N; Raugi, Dana; Sorensen, Stephanie; Genowati, Indira; Birditt, Brian; McKay, Angela; Diem, Kurt; Maust, Brandon S; Deng, Wenjie; Collier, Ann C; Stekler, Joanne D; McElrath, M Juliana; Mullins, James I

2011-08-01

HIV-1 transmission and viral evolution in the first year of infection were studied in 11 individuals representing four transmitter-recipient pairs and three independent seroconverters. Nine of these individuals were enrolled during acute infection; all were men who have sex with men (MSM) infected with HIV-1 subtype B. A total of 475 nearly full-length HIV-1 genome sequences were generated, representing on average 10 genomes per specimen at 2 to 12 visits over the first year of infection. Single founding variants with nearly homogeneous viral populations were detected in eight of the nine individuals who were enrolled during acute HIV-1 infection. Restriction to a single founder variant was not due to a lack of diversity in the transmitter as homogeneous populations were found in recipients from transmitters with chronic infection. Mutational patterns indicative of rapid viral population growth dominated during the first 5 weeks of infection and included a slight contraction of viral genetic diversity over the first 20 to 40 days. Subsequently, selection dominated, most markedly in env and nef. Mutants were detected in the first week and became consensus as early as day 21 after the onset of symptoms of primary HIV infection. We found multiple indications of cytotoxic T lymphocyte (CTL) escape mutations while reversions appeared limited. Putative escape mutations were often rapidly replaced with mutually exclusive mutations nearby, indicating the existence of a maturational escape process, possibly in adaptation to viral fitness constraints or to immune responses against new variants. We showed that establishment of HIV-1 infection is likely due to a biological mechanism that restricts transmission rather than to early adaptive evolution during acute infection. Furthermore, the diversity of HIV strains coupled with complex and individual-specific patterns of CTL escape did not reveal shared sequence characteristics of acute infection that could be harnessed for vaccine design.

On Mars' atmospheric sputtering after MAVEN first two years

NASA Astrophysics Data System (ADS)

Leblanc, F.; Modolo, R.; Curry, S.; Luhmann, J. G.; Lillis, R.; Chaufray, J. Y.; Hara, T.; McFadden, J.; Halekas, J.; Eparvier, F.; Larson, D.; Connerney, J.; Jakosky, B.

2017-09-01

Mars may have lost a significant part of its atmosphere into space along its history, in particular since the end of its internal dynamo, 4.1 Gyr ago. The sputtering of the atmosphere by precipitating planetary picked up ions accelerated by the solar wind is one of the processes that could have significantly contributed to this atmospheric escape. We here present a two years base analysis of MAVEN observation of the precipitating flux, in particular the dependency of the precipitating intensity with solar zenith angle and used this measurement to model the expected escape rate and exosphere induced by this precipitation.

Upper limits to the fractionation of isotopes due to atmospheric escape: Implications for potential 14N/15N in Pluto's atmosphere

NASA Astrophysics Data System (ADS)

Mandt, K.; Mousis, O.

2014-12-01

Formation and evolution of the solar system is studied in part using stable isotope ratios that are presumed to be primordial, or representative of conditions in the protosolar Nebula. Comets, meteorites and giant planet atmospheres provide measurements that can reasonably be presumed to represent primordial conditions while the terrestrial planets, Pluto and Saturn's moon Titan have atmospheres that have evolved over the history of the solar system. The stable isotope ratios measured in these atmospheres are, therefore, first a valuable tool for evaluating the history of atmospheric escape and once escape is constrained can provide indications of conditions of formation. D/H ratios in the atmosphere of Venus provide indications of the amount of water lost from Venus over the history of the solar system, while several isotope ratios in the atmosphere of Mars provide evidence for long-term erosion of the atmosphere. We have recently demonstrated that the nitrogen ratios, 14N/15N, in Titan's atmosphere cannot evolve significantly over the history of the solar system and that the primordial ratio for Titan must have been similar to the value recently measured for NH3 in comets. This implies that the building blocks for Titan formed in the protosolar nebula rather than in the warmer subnebula surrounding Saturn at the end of its formation. Our result strongly contrasts with works showing that 14N/15N in the atmosphere of Mars can easily fractionate from the terrestrial value to its current value due to escape processes within the lifetime of the solar system. The difference between how nitrogen fractionates in Mars and Titan's atmospheres presents a puzzle for the fractionation of isotopes in an atmosphere due to atmospheric escape. Here, we present a method aiming at determining an upper limit to the amount of fractionation allowed to occur due to escape, which is a function of the escape flux and the column density of the atmospheric constituent. Through this approach, we demonstrate that fractionation on Titan is more limited than on Mars. When applied to Pluto, we find that any potential measurement of 14N/15N in Pluto's atmosphere can constrain the type of escape occurring from Pluto's atmosphere and possibly the source of nitrogen for Pluto.

Radioactive waste disposal via electric propulsion

NASA Technical Reports Server (NTRS)

Burns, R. E.

1975-01-01

It is shown that space transportation is a feasible method of removal of radioactive wastes from the biosphere. The high decay heat of the isotopes powers a thermionic generator which provides electrical power for ion thrust engines. The massive shields (used to protect ground and flight personnel) are removed in orbit for subsequent reuse; the metallic fuel provides a shield for the avionics that guides the orbital stage to solar system escape. Performance calculations indicate that 4000 kg. of actinides may be removed per Shuttle flight. Subsidiary problems - such as cooling during ascent - are discussed.

Towards a more versatile alpha-glucan biosynthesis in plants.

PubMed

Kok-Jacon, Géraldine A; Ji, Qin; Vincken, Jean-Paul; Visser, Richard G F

2003-07-01

Starch is an important storage polysaccharide in many plants. It is composed of densely packed alpha-glucans, consisting of 1,4- and 1,4,6-linked glucose residues. The starch polymers are used in many industrial applications. The biosynthetic machinery for assembling the granule has been manipulated in many different ways to gain insight into the process of starch biosynthesis and to engineer starches with improved functionalities. With respect to the latter, two generic technologies with great potential have been developed: (i) introduction of new linkage types in starch polymers (1,3- and 1,6-linkages), and (ii) engineering granule-boundness. The toolbox to engineer this new generation of starch polymers is discussed.

The cislunar low-thrust trajectories via the libration point

NASA Astrophysics Data System (ADS)

Qu, Qingyu; Xu, Ming; Peng, Kun

2017-05-01

The low-thrust propulsion will be one of the most important propulsion in the future due to its large specific impulse. Different from traditional low-thrust trajectories (LTTs) yielded by some optimization algorithms, the gradient-based design methodology is investigated for LTTs in this paper with the help of invariant manifolds of LL1 point and Halo orbit near the LL1 point. Their deformations under solar gravitational perturbation are also presented to design LTTs in the restricted four-body model. The perturbed manifolds of LL1 point and its Halo orbit serve as the free-flight phase to reduce the fuel consumptions as much as possible. An open-loop control law is proposed, which is used to guide the spacecraft escaping from Earth or captured by Moon. By using a two-dimensional search strategy, the ON/OFF time of the low-thrust engine in the Earth-escaping and Moon-captured phases can be obtained. The numerical implementations show that the LTTs achieved in this paper are consistent with the one adopted by the SMART-1 mission.

Polymeric micelles based on poly(methacrylic acid) block-containing copolymers with different membrane destabilizing properties for cellular drug delivery.

PubMed

Mebarek, Naila; Aubert-Pouëssel, Anne; Gérardin, Corine; Vicente, Rita; Devoisselle, Jean-Marie; Bégu, Sylvie

2013-10-01

Poly(methacrylic acid)-b-poly(ethylene oxide) are double hydrophilic block copolymers, which are able to form micelles by complexation with a counter-polycation, such as poly-l-lysine. A study was carried out on the ability of the copolymers to interact with model membranes as a function of their molecular weights and as a function of pH. Different behaviors were observed: high molecular weight copolymers respect the membrane integrity, whereas low molecular weight copolymers with a well-chosen asymmetry degree can induce a membrane alteration. Hence by choosing the appropriate molecular weight, micelles with distinct membrane interaction behaviors can be obtained leading to different intracellular traffics with or without endosomal escape, making them interesting tools for cell engineering. Especially micelles constituted of low molecular weight copolymers could exhibit the endosomal escape property, which opens vast therapeutic applications. Moreover micelles possess a homogeneous nanometric size and show variable properties of disassembly at acidic pH, of stability in physiological conditions, and finally of cyto-tolerance. Copyright © 2013 Elsevier B.V. All rights reserved.

13. ENGINE TEST CELL BUILDING INTERIOR. EQUIPMENT ROOM SERVING CELLS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. ENGINE TEST CELL BUILDING INTERIOR. EQUIPMENT ROOM SERVING CELLS 2 AND 4. LOOKING SOUTHEAST. - Fairchild Air Force Base, Engine Test Cell Building, Near intersection of Arnold Street & George Avenue, Spokane, Spokane County, WA

Chemical vs. biotechnological synthesis of C13-apocarotenoids: current methods, applications and perspectives.

PubMed

Cataldo, Vicente F; López, Javiera; Cárcamo, Martín; Agosin, Eduardo

2016-07-01

Apocarotenoids are natural compounds derived from the oxidative cleavage of carotenoids. Particularly, C13-apocarotenoids are volatile compounds that contribute to the aromas of different flowers and fruits and are highly valued by the Flavor and Fragrance industry. So far, the chemical synthesis of these terpenoids has dominated the industry. Nonetheless, the increasing consumer demand for more natural and sustainable processes raises an interesting opportunity for bio-production alternatives. In this regard, enzymatic biocatalysis and metabolically engineered microorganisms emerge as attractive biotechnological options. The present review summarizes promising bioengineering approaches with regard to chemical production methods for the synthesis of two families of C13-apocarotenoids: ionones/dihydroionones and damascones/damascenone. We discuss each method and its applicability, with a thorough comparative analysis for ionones, focusing on the production process, regulatory aspects, and sustainability.

Titan's plasma interaction: photoelectrons and negative ions

NASA Astrophysics Data System (ADS)

Coates, Coates; Welbrock, Anne; Desai, Ravi; Waite, Hunter

2016-06-01

We present a review of some of the most important results from the CAPS electron spectrometer.These include the role of photoelectrons and polar wind escape processes, and remarkable negative ion observations.

How Mars lost its atmosphere

NASA Technical Reports Server (NTRS)

Zahnle, Kevin

1992-01-01

There is a widespread suspicion that Mars thin atmosphere is in some way attributable to the planet's size. Another possibility is that the atmosphere was never degassed or outgassed in the first place. I prefer escape. Hydrodynamic escape (vigorous thermal escape) and impact erosion (expulsion of atmosphere by impacts) are two processes that should have been operative early. Although in principle hydrodynamic escape could have shrunk Mars atmosphere a hundredfold while leaving the composition of the remnant atmosphere nearly unaltered, very high escape fluxes are required. The implicated escape mechanism must have been efficient, nearly non-fractionating, and vastly more potent for Mars than for Earth or Venus. Impact erosion is an appealing candidate. Noble gases are the obvious first test. Noble gases are the most volatile elements and so are the most likely to have been affected by impact erosion and the easiest to address quantitatively. Xenon in particular imposes three constraints on how Mars lost its atmosphere: (1) the very low abundance of nonradiogenic Xe abundance of nonradiogenic Xe compared to Earth, Venus, and likely meteoritic sources; (2) its nonradiogenic isotopes distinct from likely meteoritic sources; and (3) the relatively high absolute abundance of radiogenic daughter of the extinct radionuclide I-129 (half-life 17 Myr). In impact erosion, the first two become constraints on the composition, mass distribution, and orbital elements of the impactors. The third requires that Mars lost its nonradiogenic Xe early, probably before it was 100 Myr old. Impact erosion can explain Mars by any of three stories. (1) Mars in unlikely. In a sort of planetary brinkmanship, impact erosion almost removed the entire atmosphere but was arrested just in time. (2) Martian noble gases are cometary and cometary Xe is as isotopically mass fractionated as Martian and terrestrial Xe. This is most easily accomplished if a relatively thick geochemically controlled CO2 atmosphere protected trace atmophiles against escape. (3) Mars was indeed stripped of its early atmosphere but a small remnant was safely stored in the regolith, later released as a byproduct of water mobilization.

Oxygen Loss from Venus and the Influence of Extreme Solar Wind Conditions

NASA Astrophysics Data System (ADS)

McEnulty, Tess Rose

2012-06-01

The purpose of this dissertation is to expand our understanding of oxygen ion escape to space from Venus and its dependence on extreme solar wind conditions found during interplanetary coronal mass ejections (ICMEs). The solar wind dynamic pressure outside of the Venus bow shock did not exceed ˜12 nPa, during 2006-2009, while the solar wind dynamic pressure was higher than this for ˜10% of the time during the PVO mission. Oxygen ions escape Venus through multiple regions near the planet. One of these regions is the magnetosheath, where high energy pick-up ions are accelerated by the solar wind convection electric field. High energy (>1 keV) O+ pick-up ions within the Venus magnetosheath reached higher energy at lower altitude when the solar wind was disturbed by ICMEs compared to pick-up ions when the external solar wind was not disturbed, between 2006-2007. However, the count rate of O+ was not obviously affected by the ICMEs during this time period. In addition to high energy pick-up ions, VEX also detects low energy (˜10-100 eV) O+ within the ionosphere and wake of Venus. These low energy oxygen ions are difficult to interpret, because the spacecraft's relative velocity and potential can significantly affect the measured energy. If VEX ion data is not corrected for the spacecraft's relative velocity and potential, gravitationally bound O+ could be misinterpreted as escaping. These gravitationally bound oxygen ions can extend on the nightside to ˜-2 Venus radii and may even return to the planet after reaching high altitudes in the wake. Gravitationally bound ions will lower the total O+ escape estimated from Venus if total escape is calculated including these ions. However, if the return flux is low compared to the total escaping outflow, this effect is not significant. An ICME with a dynamic pressure of 17.6 nPa impacted Venus on November 11, 2011. During this ICME, the high energy pick-up O+ and the low energy O+ ions were affected. Oxygen ions in the magnetosheath, ionosphere, and tail had higher energies during the ICME, compared to O + energies when the external solar wind conditions were undisturbed. High energy ions were escaping within the dayside magnetosheath region when the ICME was passing as well as when the solar wind was undisturbed. However, during the ICME passage, these O+ ions had three orders of magnitude higher counts. The low energy O+ during the undisturbed days was gravitationally bound, while during the ICME a portion of the low energy ions were likely escaping. The most significant difference in O + during the ICME was high energy pickup ions measured in the wake on the outbound portion of the orbit. These ions had an escape flux of 2.5 X 108 O+cm-2sec-1, which is higher than the average escape flux in all regions of the wake. In addition, the interplanetary magnetic field (IMF) was in a configuration that may have rotated an even higher escape flux O+ away from the VEX orbit. This needs to be confirmed with sampling of other regions in the wake during large ICMEs. A lower bound on the total O+ escape during this event could be ˜2.8 X1026 to 6.5 X 1027 O +/sec, which is 2-3 orders of magnitude higher than the average escape flux measured by VEX. Hence, ICMEs could have played a major role in the total escape of O+ from Venus. The results presented in this dissertation can be used as a guide for future studies of O+ escape at Venus. As we move into solar maximum, Venus will likely be impacted by more large ICMEs. The ICME from the last study of this dissertation was the largest yet measured by VEX, but its 17.6 nPa dynamic pressure is lower than the largest ICMEs during the PVO time period (˜ 80 nPa). The work in this dissertation is also relevant to Mars, since Mars interacts with the solar wind in a similar manner and has analogous ion escape mechanisms. The upcoming MAVEN (Mars Atmosphere and Volatile Evolution) mission will launch at the end of 2013 to study the Martian atmosphere, escape processes, and history of volatiles. This mission will have an in-situ ion instrument and magnetometer similar to those used for the studies in this dissertation, so one could conduct similar studies of the oxygen ion escape from Mars during extreme solar wind conditions. (Abstract shortened by UMI.)

Statistics of indicated pressure in combustion engine.

NASA Astrophysics Data System (ADS)

Sitnik, L. J.; Andrych-Zalewska, M.

2016-09-01

The paper presents the classic form of pressure waveforms in burn chamber of diesel engine but based on strict analytical basis for amending the displacement volume. The pressure measurement results are obtained in the engine running on an engine dynamometer stand. The study was conducted by a 13-phase ESC test (European Stationary Cycle). In each test phase are archived 90 waveforms of pressure. As a result of extensive statistical analysis was found that while the engine is idling distribution of 90 value of pressure at any value of the angle of rotation of the crankshaft can be described uniform distribution. In the each point of characteristic of the engine corresponding to the individual phases of the ESC test, 90 of the pressure for any value of the angle of rotation of the crankshaft can be described as normal distribution. These relationships are verified using tests: Shapiro-Wilk, Jarque-Bera, Lilliefors, Anderson-Darling. In the following part, with each value of the crank angle, are obtain values of descriptive statistics for the pressure data. In its essence, are obtained a new way to approach the issue of pressure waveform analysis in the burn chamber of engine. The new method can be used to further analysis, especially the combustion process in the engine. It was found, e.g. a very large variances of pressure near the transition from compression to expansion stroke. This lack of stationarity of the process can be important both because of the emissions of exhaust gases and fuel consumption of the engine.

Relationship of scores on the Escapism Scale of the MMPI to escape from minimum security federal custody.

PubMed

White, R B

1979-04-01

Investigated the ability of the Escapism (Ec) scale of the MMPI to differentiate between escape and non-escape minimum security federal prisoners. At the .05 level there was no difference between the scores of the two groups on the Ec scale or on comparisons of other correctional data, age, and ethnic composition. It appears that the Ec scale alone or in combination with other data will be a poor predictor of escape. Also, the rate of escape was so low as to make accurate prediction from any criteria extremely unlikely.

Mars atmospheric escape and evolution; interaction with the solar wind

NASA Astrophysics Data System (ADS)

Chassefière, Eric; Leblanc, François

2004-09-01

This tutorial deals with the question of atmospheric escape on Mars. After a brief introduction describing the general context of Mars escape studies, we will present in Section 2 a simplified theory of thermal escape, of both Jeans and hydrodynamic types. The phenomenon of hydrodynamic escape, still hypothetical and not proved to have ever existed on terrestrial planets, will be treated with the help of two well known examples: (i) the isotopic fractionation of xenon in Mars and Earth atmospheres, (ii) the paradox of missing oxygen in Venus atmosphere. In Section 3, a simplified approach of non-thermal escape will be developed, treating in a specific way the different kinds of escape (photochemical escape, ion sputtering, ion escape and ionospheric outflow). As a matter of illustration, some calculations of the relative contributions of these mechanisms, and of their time evolutions, will be given, and the magnitude of the total amount of atmosphere lost by non-thermal escape will be estimated. Section 4 will present the state of knowledge concerning the constraints derived from Mars isotopic geochemistry in terms of past escape and evolution. Finally, a few conclusions, which are more interrogations, will be proposed.

Proceedings: Workshop on the Need for Lightning Observations from Space

NASA Technical Reports Server (NTRS)

Christensen, L. S. (Editor); Frost, W. (Editor); Vaughan, W. W. (Editor)

1979-01-01

The results of the Workshop on the Need for Lightning Observations from Space held February 13-15, 1979, at the University of Tennessee Space Institute, Tullahoma, Tennessee are presented. The interest and active involvement by the engineering, operational, and scientific participants in the workshop demonstrated that lightning observations from space is a goal well worth pursuing. The unique contributions, measurement requirements, and supportive research investigations were defined for a number of important applications. Lightning has a significant role in atmospheric processes and needs to be systematically investigated. Satellite instrumentation specifically designed for indicating the characteristics of lightning are of value in severe storms research, in engineering and operational problem areas, and in providing information on atmospheric electricity and its role in meteorological processes.

How embryos escape from danger: the mechanism of rapid, plastic hatching in red-eyed treefrogs.

PubMed

Cohen, Kristina L; Seid, Marc A; Warkentin, Karen M

2016-06-15

Environmentally cued hatching allows embryos to escape dangers and exploit new opportunities. Such adaptive responses require a flexibly regulated hatching mechanism sufficiently fast to meet relevant challenges. Anurans show widespread, diverse cued hatching responses, but their described hatching mechanisms are slow, and regulation of timing is unknown. Arboreal embryos of red-eyed treefrogs, Agalychnis callidryas, escape from snake attacks and other threats by very rapid premature hatching. We used videography, manipulation of hatching embryos and electron microscopy to investigate their hatching mechanism. High-speed video revealed three stages of the hatching process: pre-rupture shaking and gaping, vitelline membrane rupture near the snout, and muscular thrashing to exit through the hole. Hatching took 6.5-49 s. We hypothesized membrane rupture to be enzymatic, with hatching enzyme released from the snout during shaking. To test this, we displaced hatching embryos to move their snout from its location during shaking. The membrane ruptured at the original snout position and embryos became trapped in collapsed capsules; they either moved repeatedly to relocate the hole or shook again and made a second hole to exit. Electron microscopy revealed that hatching glands are densely concentrated on the snout and absent elsewhere. They are full of vesicles in embryos and release most of their contents rapidly at hatching. Agalychnis callidryas' hatching mechanism contrasts with the slow process described in anurans to date and exemplifies one way in which embryos can achieve rapid, flexibly timed hatching to escape from acute threats. Other amphibians with cued hatching may also have novel hatching mechanisms. © 2016. Published by The Company of Biologists Ltd.

Regulatory and evolutionary signatures of sex-biased genes on both the X chromosome and the autosomes.

PubMed

Shen, Jiangshan J; Wang, Ting-You; Yang, Wanling

2017-11-02

Sex is an important but understudied factor in the genetics of human diseases. Analyses using a combination of gene expression data, ENCODE data, and evolutionary data of sex-biased gene expression in human tissues can give insight into the regulatory and evolutionary forces acting on sex-biased genes. In this study, we analyzed the differentially expressed genes between males and females. On the X chromosome, we used a novel method and investigated the status of genes that escape X-chromosome inactivation (escape genes), taking into account the clonality of lymphoblastoid cell lines (LCLs). To investigate the regulation of sex-biased differentially expressed genes (sDEG), we conducted pathway and transcription factor enrichment analyses on the sDEGs, as well as analyses on the genomic distribution of sDEGs. Evolutionary analyses were also conducted on both sDEGs and escape genes. Genome-wide, we characterized differential gene expression between sexes in 462 RNA-seq samples and identified 587 sex-biased genes, or 3.2% of the genes surveyed. On the X chromosome, sDEGs were distributed in evolutionary strata in a similar pattern as escape genes. We found a trend of negative correlation between the gene expression breadth and nonsynonymous over synonymous mutation (dN/dS) ratios, showing a possible pleiotropic constraint on evolution of genes. Genome-wide, nine transcription factors were found enriched in binding to the regions surrounding the transcription start sites of female-biased genes. Many pathways and protein domains were enriched in sex-biased genes, some of which hint at sex-biased physiological processes. These findings lend insight into the regulatory and evolutionary forces shaping sex-biased gene expression and their involvement in the physiological and pathological processes in human health and diseases.

Escape of Hydrogen from the Exosphere of Mars

NASA Astrophysics Data System (ADS)

Bhattacharyya, Dolon; Clarke, John T.; Bertaux, Jean-Loup; Chaufray, Jean-Yves; Mayyasi-Matta, Majd A.

2016-10-01

After decades of exploration, the martian neutral hydrogen exosphere has remained largely uncharacterized even today. In my dissertation I have attempted to constrain the characteristics of the martian hydrogen exosphere using Hubble Space Telescope observations obtained during October-November 2007 and 2014. These observations reveal short-term seasonal changes exhibited by the martian hydrogen exosphere that are inconsistent with the diffusion-limited escape scenario. This seasonal behavior adds a new element towards backtracking the history of water loss from Mars. Modeling of the data also indicates the likely presence of a superthermal population of hydrogen created by non-thermal processes at Mars, another key element to understand the present-day escape. Exploration of the latitudinal symmetry of the martian exosphere indicates that it is symmetric above 2.5 martian radii and asymmetric below this altitude, which could be due to temperature differences between the day and night sides. Finally, the large uncertainties in determining the characteristics of the martian exosphere after decades of exploration is due to various assumptions about the intrinsic characteristics of the martian exosphere in the modeling process, degeneracy in the two modeling parameters temperature and density of the hydrogen atoms, unaccounted seasonal effects, and uncertainties introduced from spacecraft instrumentation as well as their viewing geometry.

50 CFR Appendix D to Part 622 - Specifications for Certified BRDs

Code of Federal Regulations, 2010 CFR

2010-10-01

... (7.6-cm) piece of 1/2-inch (1.3-cm) aluminum pipe and pressed with a 3/8-inch (0.95-cm) die to form a... must be constructed of either number 60 twine 80 inches (203.2 cm) in length or 3/8-inch (0.95-cm... excluder device (TED) grid; and, (4) the area of the escape opening must total at least 864 in2 (5,574.2...

JPRS Report, Science & Technology, USSR: Chemistry.

DTIC Science & Technology

1988-12-23

Escape Accidents at Nuclear Power Plant With Water Moderated Reactors [S. A. Kabakehi, M. A. Budayev, et al.; KHIMIYA VYSOKIKH ENERGIY, Vol 22 No 4...water on the reactor surface also lowered loss of radicals. In general, the extent and temperature function Study of Radicals Desorbing From Surface... reactor sur- Alcohols With Oxygen face, by adsorption of the reagents and reaction products 184100 13f Moscow KHIMICHESKA YA FIZIKA on this surface and the

Distortion-free foamed-plastic parts

NASA Technical Reports Server (NTRS)

Hogenson, P. A.; Jackson, R. G.

1979-01-01

In process for molding foamed-plastic products, gases that are formed as byproducts of foaming reaction escape through perforated die. Thus, volatiles are not trapped in pockets that can deform and weaken the molded part.

Espisodic detachment of Martian crustal magnetic fields leading to bulk atmospheric plasma escape

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brain, D A; Baker, A H; Briggs, J

2009-06-02

We present an analysis of magnetic field and suprathermal electron measurements from the Mars Global Surveyor (MGS) spacecraft that reveals isolated magnetic structures filled with Martian atmospheric plasma located downstream from strong crustal magnetic fields with respect to the flowing solar wind. The structures are characterized by magnetic field enhancements and rotations characteristic of magnetic flux ropes, and characteristic ionospheric electron energy distributions with angular distributions distinct from surrounding regions. These observations indicate that significant amounts of atmosphere are intermittently being carried away from Mars by a bulk removal process: the top portions of crustal field loops are stretched throughmore » interaction with the solar wind and detach via magnetic reconnection. This process occurs frequently and may account for as much as 10% of the total present-day ion escape from Mars.« less

Neural responses from the wind-sensitive interneuron population in four cockroach species

PubMed Central

McGorry, Clare A.; Newman, Caroline N.; Triblehorn, Jeffrey D.

2014-01-01

The wind-sensitive insect cercal sensory system is involved in important behaviors including predator detection and initiating terrestrial escape responses as well as flight maintenance. However, not all insects possessing a cercal system exhibit these behaviors. In cockroaches, wind evokes strong terrestrial escape responses in Periplaneta americana and Blattella germanica, but only weak escape responses in Blaberus craniifer and no escape responses in Gromphadorhina portentosa. Both P. americana and Blab. craniifer possesses pink flight muscles correlated with flight ability while Blat. germanica possesses white flight muscles that cannot support flight and G. portentosa lacks wings. These different behavioral combinations could correlate with differences in sensory processing of wind information by the cercal system. In this study, we focused on the wind-sensitive interneurons (WSIs) since they provide input to the premotor/motor neurons that influence terrestrial escape and flight behavior. Using extracellular recordings, we characterized the responses from the WSI population by generating stimulus-response (S-R) curves and examining spike firing rates. Using cluster analysis, we also examined the activity of individual units (four per species, though not necessarily homologous) comprising the population response in each species. Our main results were: 1) all four species possessed ascending WSIs in the abdominal connectives; 2) wind elicited the weakest WSI responses (lowest spike counts and spike rates) in G. portentosa; 3) wind elicited WSI responses in Blab. craniifer that were greater than P. americana or Blat. germanica; 4) the activity of four individual units comprising the WSI population response in each species was similar across species. PMID:24879967

Seasonal variability of Martian ion escape through the plume and tail from MAVEN observations

NASA Astrophysics Data System (ADS)

Dong, Y.; Fang, X.; Brain, D. A.; McFadden, J. P.; Halekas, J. S.; Connerney, J. E. P.; Eparvier, F.; Andersson, L.; Mitchell, D.; Jakosky, B. M.

2017-04-01

We study the Mars Atmosphere and Volatile Evolution spacecraft observations of Martian planetary ion escape during two time periods: 11 November 2014 to 19 March 2015 and 4 June 2015 to 24 October 2015, with the focus on understanding the seasonal variability of Martian ion escape in response to the solar extreme ultraviolet (EUV) flux. We organize the >6 eV O+ ion data by the upstream electric field direction to estimate the escape rates through the plume and tail. To investigate the ion escape dependence on the solar EUV flux, we constrain the solar wind dynamic pressure and interplanetary magnetic filed strength and compare the ion escape rates through the plume and tail in different energy ranges under high and low EUV conditions. We found that the total >6 eV O+ escape rate increases from 2 to 3 × 1024 s-1 as the EUV irradiance increases by almost the same factor, mostly on the <1 keV tailward escape. The plume escape rate does not vary significantly with EUV. The relative contribution from the plume to the total escape varies between 30% and 20% from low to high EUV. Our results suggest that the Martian ion escape is sensitive to the seasonal EUV variation, and the contribution from plume escape becomes more important under low EUV conditions.

Diversity of the Lyman continuum escape fractions of high-z galaxies and its origins

NASA Astrophysics Data System (ADS)

Sumida, Takumi; Kashino, Daichi; Hasegawa, Kenji

2018-04-01

The Lyman continuum (LyC) escape fraction is a key quantity to determine the contribution of galaxies to cosmic reionization. It has been known that the escape fractions estimated by observations and numerical simulations show a large diversity. However, the origins of the diversity are still uncertain. In this work, to understand what quantities of galaxies are responsible for controlling the escape fraction, we numerically evaluate the escape fraction by performing ray-tracing calculation with simplified disc galaxy models. With a smooth disc model, we explore the dependence of the escape fraction on the disposition of ionizing sources and find that the escape fraction varies up to ˜3 orders of magnitude. It is also found that the halo mass dependence of disc scale height determines whether the escape fraction increases or decreases with halo mass. With a clumpy disc model, it turns out that the escape fraction increases as the clump mass fraction increases because the density in the inter-clump region decreases. In addition, we find that clumpiness regulates the escape fraction via two ways when the total clump mass dominates the total gas mass; the escape fraction is controlled by the covering factor of clumps if the clumps are dense sufficient to block LyC photons, otherwise the clumpiness works to reduce the escape fraction by increasing the total number of recombination events in a galaxy.

Engineering controllable bidirectional molecular motors based on myosin

PubMed Central

Chen, Lu; Nakamura, Muneaki; Schindler, Tony D.; Parker, David; Bryant, Zev

2012-01-01

Cytoskeletal motors drive the transport of organelles and molecular cargoes within cells1, and have potential applications in molecular detection and diagnostic devices2,3. Engineering molecular motors with dynamically controllable properties will allow selective perturbation of mechanical processes in living cells, and yield optimized device components for complex tasks such as molecular sorting and directed assembly3. Biological motors have previously been modified by introducing activation/deactivation switches that respond to metal ions4,5 and other signals6. Here we show that myosin motors can be engineered to reversibly change their direction of motion in response to a calcium signal. Building on previous protein engineering studies7–11 and guided by a structural model12 for the redirected power stroke of myosin VI, we constructed bidirectional myosins through the rigid recombination of structural modules. The performance of the motors was confirmed using gliding filament assays and single fluorophore tracking. Our general strategy, in which external signals trigger changes in the geometry and mechanics of myosin lever arms, should enable spatiotemporal control over a range of motor properties including processivity, stride size13, and branchpoint turning14. PMID:22343382

XUV-Exposed, Non-Hydrostatic Hydrogen-Rich Upper Atmospheres of Terrestrial Planets. Part II: Hydrogen Coronae and Ion Escape

PubMed Central

Lammer, Helmut; Holmström, Mats; Panchenko, Mykhaylo; Odert, Petra; Erkaev, Nikolai V.; Leitzinger, Martin; Khodachenko, Maxim L.; Kulikov, Yuri N.; Güdel, Manuel; Hanslmeier, Arnold

2013-01-01

Abstract We studied the interactions between the stellar wind plasma flow of a typical M star, such as GJ 436, and the hydrogen-rich upper atmosphere of an Earth-like planet and a “super-Earth” with a radius of 2 REarth and a mass of 10 MEarth, located within the habitable zone at ∼0.24 AU. We investigated the formation of extended atomic hydrogen coronae under the influences of the stellar XUV flux (soft X-rays and EUV), stellar wind density and velocity, shape of a planetary obstacle (e.g., magnetosphere, ionopause), and the loss of planetary pickup ions on the evolution of hydrogen-dominated upper atmospheres. Stellar XUV fluxes that are 1, 10, 50, and 100 times higher compared to that of the present-day Sun were considered, and the formation of high-energy neutral hydrogen clouds around the planets due to the charge-exchange reaction under various stellar conditions was modeled. Charge-exchange between stellar wind protons with planetary hydrogen atoms, and photoionization, lead to the production of initially cold ions of planetary origin. We found that the ion production rates for the studied planets can vary over a wide range, from ∼1.0×1025 s−1 to ∼5.3×1030 s−1, depending on the stellar wind conditions and the assumed XUV exposure of the upper atmosphere. Our findings indicate that most likely the majority of these planetary ions are picked up by the stellar wind and lost from the planet. Finally, we estimated the long-time nonthermal ion pickup escape for the studied planets and compared them with the thermal escape. According to our estimates, nonthermal escape of picked-up ionized hydrogen atoms over a planet's lifetime within the habitable zone of an M dwarf varies between ∼0.4 Earth ocean equivalent amounts of hydrogen (EOH) to <3 EOH and usually is several times smaller in comparison to the thermal atmospheric escape rates. Key Words: Stellar activity—Low-mass stars—Early atmospheres—Earth-like exoplanets—Energetic neutral atoms—Ion escape—Habitability. Astrobiology 13, 1030–1048. PMID:24283926

46 CFR 185.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

46 CFR 185.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

46 CFR 185.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

46 CFR 185.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

46 CFR 185.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Escape hatches and emergency exits. 185.606 Section 185... 100 GROSS TONS) OPERATIONS Markings Required § 185.606 Escape hatches and emergency exits. All escape hatches and other emergency exits used as means of escape must be marked on both sides in clearly legible...

Escape as Reinforcement and Escape Extinction in the Treatment of Feeding Problems

ERIC Educational Resources Information Center

LaRue, Robert H.; Stewart, Victoria; Piazza, Cathleen C.; Volkert, Valerie M.; Patel, Meeta R.; Zeleny, Jason

2011-01-01

Given the effectiveness of putative escape extinction as treatment for feeding problems, it is surprising that little is known about the effects of escape as reinforcement for appropriate eating during treatment. In the current investigation, we examined the effectiveness of escape as reinforcement for mouth clean (a product measure of…

Decompression illness in goats following simulated submarine escape: 1993-2006.

PubMed

Seddon, F M; Thacker, J C; Fisher, A S; Jurd, K M; White, M G; Loveman, G A M

2014-01-01

The United Kingdom Ministry of Defence commissioned work to define the relationship between the internal pressure of a distressed submarine (DISSUB), the depth from which escape is made and the risk of decompression illness (DCI). The program of work used an animal model (goat) to define these risks and this paper reports the incidence and type of DCI observed. A total of 748 pressure exposures comprising saturation only, escape only or saturation followed by escape were conducted in the submarine escape simulator between 1993 and 2006. The DCI following saturation exposures was predominantly limb pain, whereas following escape exposures the DCI predominantly involved the central nervous system and was fast in onset. There was no strong relationship between the risk of DCI and the range of escape depths investigated. The risk of DCI incurred from escape following saturation was greater than that obtained by combining the risks for the independent saturation only, and escape only, exposures. The output from this program of work has led to improved advice on the safety of submarine escape.

Radiotelemetry to estimate stream life of adult chum salmon in the McNeil River, Alaska

USGS Publications Warehouse

Peirce, Joshua M.; Otis, Edward O.; Wipfli, Mark S.; Follmann, Erich H.

2011-01-01

Estimating salmon escapement is one of the fundamental steps in managing salmon populations. The area-under-the-curve (AUC) method is commonly used to convert periodic aerial survey counts into annual salmon escapement indices. The AUC requires obtaining accurate estimates of stream life (SL) for target species. Traditional methods for estimating SL (e.g., mark–recapture) are not feasible for many populations. Our objective in this study was to determine the average SL of chum salmon Oncorhynchus keta in the McNeil River, Alaska, through radiotelemetry. During the 2005 and 2006 runs, 155 chum salmon were fitted with mortality-indicating radio tags as they entered the McNeil River and tracked until they died. A combination of remote data loggers, aerial surveys, and foot surveys were used to determine the location of fish and provide an estimate of time of death. Higher predation resulted in tagged fish below McNeil Falls having a significantly shorter SL (12.6 d) than those above (21.9 d). The streamwide average SL (13.8 d) for chum salmon at the McNeil River was lower than the regionwide value (17.5 d) previously used to generate AUC indices of chum salmon escapement for the McNeil River. We conclude that radiotelemetry is an effective tool for estimating SL in rivers not well suited to other methods.

Space Shuttle Hot Cabin Emergency Responses

NASA Technical Reports Server (NTRS)

Stepaniak, P.; Effenhauser, R. K.; McCluskey, R.; Gillis, D. B.; Hamilton, D.; Kuznetz, L. H.

2005-01-01

Methods: Human thermal tolerance, countermeasures, and thermal model data were reviewed and compared to existing shuttle ECS failure temperature and humidity profiles for each failure mode. Increases in core temperature associated with cognitive impairment was identified, as was metabolic heat generation of crewmembers, temperature monitoring, and communication capabilities after partial power-down and other limiting factors. Orbiter landing strategies and a hydration and salt replacement protocol were developed to put wheels on deck in each failure mode prior to development of significant cognitive impairment or collapse of crewmembers. Thermal tradeoffs for use of the Advanced Crew Escape Suit (ACES), Liquid Cooling Garment, integrated G-suit and Quick Don Mask were examined. candidate solutions involved trade-offs or conflicts with cabin oxygen partial pressure limits, system power-downs to limit heat generation, risks of alternate and emergency landing sites or compromise of Mode V-VIII scenarios. Results: Rehydration and minimized cabin workloads are required in all failure modes. Temperature/humidity profiles increase rapidly in two failure modes, and deorbit is recommended without the ACES, ICU and g-suit. This latter configuration limits several shuttle approach and landing escape modes and requires communication modifications. Additional data requirements were identified and engineering simulations were recommended to develop more current shuttle temperature and humidity profiles. Discussion: After failure of the shuttle ECS, there is insufficient cooling capacity of the ACES to protect crewmembers from rising cabin temperature and humidity. The LCG is inadequate for cabin temperatures above 76 F. Current shuttle future life policy makes it unlikely that major engineering upgrades necessary to address this problem will occur.

The GRB-SLSN connection: misaligned magnetars, weak jet emergence, and observational signatures

NASA Astrophysics Data System (ADS)

Margalit, Ben; Metzger, Brian D.; Thompson, Todd A.; Nicholl, Matt; Sukhbold, Tuguldur

2018-04-01

Multiple lines of evidence support a connection between hydrogen-poor superluminous supernovae (SLSNe) and long-duration gamma-ray bursts (GRBs). Both classes of events require a powerful central energy source, usually attributed to a millisecond magnetar or an accreting black hole. The GRB-SLSN link raises several theoretical questions: What distinguishes the engines responsible for these different phenomena? Can a single engine power both a GRB and a luminous SN in the same event? We propose a unifying model for magnetar thermalization and jet formation: misalignment between the rotation (Ω) and magnetic dipole (μ) axes dissipates a fraction of the spin-down power by reconnection in the striped equatorial wind, providing a guaranteed source of `thermal' emission to power the supernova. The remaining unthermalized power energizes a relativistic jet. We show that even weak relativistic jets of luminosity ˜1046 erg s-1 can escape the expanding SN ejecta implying that escaping relativistic jets may accompany many SLSNe. We calculate the observational signature of these jets. We show that they may produce transient ultraviolet (UV) cocoon emission lasting a few hours when the jet breaks out of the ejecta surface. A longer lived optical/UV signal may originate from a mildly relativistic wind driven from the interface between the jet and the ejecta walls, which could explain the secondary early-time maximum observed in some SLSNe light curves, such as LSQ14bdq. Our scenario predicts a population of GRB from on-axis jets with extremely long durations, potentially similar to the population of `jetted-tidal disruption events', in coincidence with a small subset of SLSNe.

Analysis of Titan's neutral upper atmosphere from Cassini Ion Neutral Mass Spectrometer measurements in the Closed Source Neutral mode

NASA Astrophysics Data System (ADS)

Cui, Jun

In this thesis I present an in-depth study of the distribution of various neutral species in Titan's upper atmosphere, at altitudes between 950 and 1,500 km for abundant species (N 2 , CH 4 as well as their isotopes) and between 950 and 1,200 km for most minor species. However, the study of the H 2 distribution on Titan is extended to an altitude as high as 6,000 km in the exosphere. The analysis is based on a large sample of Cassini/INMS (Ion Neutral Mass Spectrometer) measurements in the CSN (Closed Source Neutral) mode, obtained during 15 close flybys of Titan. The densities of abundant species including N 2 , CH 4 and H 2 are determined directly from their main channels. However, to untangle the overlapping cracking patterns of minor species, the technique of Singular Value Decomposition (SVD) is used to determine simultaneously the densities of various hydrocarbons, nitriles and oxygen compounds. All minor species except for 40 Ar present density enhancements measured during the outbound legs. This can be interpreted as a result of wall effects, which could be either adsorption/desorption or heterogeneous surface chemistry on the chamber walls. In the thesis, I use a simple model to describe the observed time behavior of minor species. Results on their atmospheric abundances are provided both in terms of direct inbound measurements assuming ram pressure enhancement and values corrected for wall adsorption/desorption. Among all minor species of photochemical interest, the INMS data provide direct observational evidences for C 2 H 2 , C 2 H 4 , C 2 H 6 , CH 3 C 2 H, C 4 H 2 , C 6 H 6 , HC 3 N and C 2 N 2 in Titan's upper atmosphere. Upper limits are put for other minor species. The globally averaged distribution of N 2 , CH 4 and H 2 are each modeled with the diffusion approximation. The N 2 profile suggests an average thermospheric temperature of 154 K. The CH 4 and H 2 distribution constrains their fluxes to be 3.0 × 10 9 cm -2 s -1 and 1.3 × 10 10 cm -2 s -1 , referred to Titan's surface. The H 2 escape flux is about a factor of ~3 higher than the Jeans value, which is interpreted as enhanced thermal escape driven primarily by an upward conductive heat flux. Such a conclusion is based on kinetic model calculations in the 13-moment approximation that require energy continuity at the upper boundary. On the other hand, a proper interpretation of the observed CH 4 escape has to rely on the detailed nonthermal processes, which are still unknown at the present time. The INMS observations of the nitrogen isotope ratio implies 14 N/ 15 N=131.6 near Titan's surface. The profile of carbon isotope ratio combining INMS and GCMS results implies that both CH 4 and its isotope escape from Titan's exobase with roughly the same drift velocity, in contrast to the Jeans case which requires that CH 4 escapes with a much larger velocity due to its smaller mass. The INMS data also suggest horizontal/diurnal variations of temperature and neutral gas distribution in Titan's thermosphere. The equatorial regions, the ramside, as well as the nightside hemisphere of Titan appear to be warmer and present some evidences for the depletion of light species such as CH 4 . Meridional variations of most heavy species are also observed, with a trend of depletion toward the north pole. Though some of the above variations might be interpreted by either the solar-driven models or plasma-driven models, a physical scenario that reconciles all the observed horizontal/diurnal variations in a consistent way is still missing, With a careful evaluation of the effect of restricted sampling, some of the features shown in the INMS data are more likely to be observational biases.

Identifying potential misfit items in cognitive process of learning engineering mathematics based on Rasch model

NASA Astrophysics Data System (ADS)

Ataei, Sh; Mahmud, Z.; Khalid, M. N.

2014-04-01

The students learning outcomes clarify what students should know and be able to demonstrate after completing their course. So, one of the issues on the process of teaching and learning is how to assess students' learning. This paper describes an application of the dichotomous Rasch measurement model in measuring the cognitive process of engineering students' learning of mathematics. This study provides insights into the perspective of 54 engineering students' cognitive ability in learning Calculus III based on Bloom's Taxonomy on 31 items. The results denote that some of the examination questions are either too difficult or too easy for the majority of the students. This analysis yields FIT statistics which are able to identify if there is data departure from the Rasch theoretical model. The study has identified some potential misfit items based on the measurement of ZSTD where the removal misfit item was accomplished based on the MNSQ outfit of above 1.3 or less than 0.7 logit. Therefore, it is recommended that these items be reviewed or revised to better match the range of students' ability in the respective course.

Routes to formation of highly excited neutral atoms in the break-up of strongly driven hydrogen molecule

NASA Astrophysics Data System (ADS)

Emmanouilidou, Agapi

2012-06-01

We present a theoretical quasiclassical treatment of the formation, during Coulomb explosion, of highly excited neutral H atoms for strongly-driven hydrogen molecule. This process, where after the laser field is turned off, one electron escapes to the continuum while the other occupies a Rydberg state, was recently reported in an experimental study in Phys. Rev. Lett 102, 113002 (2009). We find that two-electron effects are important in order to correctly account for all pathways leading to highly excited neutral hydrogen formation [1]. We identify two pathways where the electron that escapes to the continuum does so either very quickly or after remaining bound for a few periods of the laser field. These two pathways of highly excited neutral H formation have distinct traces in the probability distribution of the escaping electron momentum components. [4pt] [1] A. Emmanouilidou, C. Lazarou, A. Staudte and U. Eichmann, Phys. Rev. A (Rapid) 85 011402 (2012).

Chitosugar translocation by an unexpressed monomeric protein channel

NASA Astrophysics Data System (ADS)

Soysa, H. Sasimali M.; Suginta, Wipa; Moonsap, Watcharaporn; Smith, M. F.

2018-05-01

The outer membrane protein channel Ec ChiP , associated with a silent gene in E . coli, is a monomeric chitoporin. In a glucose-deficient environment, E . coli can express the ChiP gene to exploit chitin degradation products. Single-channel small ion current measurements, which reveal the dynamics of single sugar molecules trapped in channel, are used here to study the exotic transport of chitosugars by E . coli. Molecules escape from the channel on multiple timescales. Voltage-dependent trapping rates observed for charged chitosan molecules, as well as model calculations, indicate that the rapid escape processes are those in which the molecule escapes back to the side of the membrane from which it originated. The probability that a sugar molecule is translocated through the membrane is thus estimated from the current data and the dependence of this translocation probability on the length of the chitosugar molecule and the applied voltage analyzed. The described method for obtaining the translocation probability and related molecular translocation current is applicable to other transport channels.

The escape problem for mortal walkers

NASA Astrophysics Data System (ADS)

Grebenkov, D. S.; Rupprecht, J.-F.

2017-02-01

We introduce and investigate the escape problem for random walkers that may eventually die, decay, bleach, or lose activity during their diffusion towards an escape or reactive region on the boundary of a confining domain. In the case of a first-order kinetics (i.e., exponentially distributed lifetimes), we study the effect of the associated death rate onto the survival probability, the exit probability, and the mean first passage time. We derive the upper and lower bounds and some approximations for these quantities. We reveal three asymptotic regimes of small, intermediate, and large death rates. General estimates and asymptotics are compared to several explicit solutions for simple domains and to numerical simulations. These results allow one to account for stochastic photobleaching of fluorescent tracers in bio-imaging, degradation of mRNA molecules in genetic translation mechanisms, or high mortality rates of spermatozoa in the fertilization process. Our findings provide a mathematical ground for optimizing storage containers and materials to reduce the risk of leakage of dangerous chemicals or nuclear wastes.

Stability of aerosol droplets in Bessel beam optical traps under constant and pulsed external forces

NASA Astrophysics Data System (ADS)

David, Grégory; Esat, Kıvanç; Hartweg, Sebastian; Cremer, Johannes; Chasovskikh, Egor; Signorell, Ruth

2015-04-01

We report on the dynamics of aerosol droplets in optical traps under the influence of additional constant and pulsed external forces. Experimental results are compared with simulations of the three-dimensional droplet dynamics for two types of optical traps, the counter-propagating Bessel beam (CPBB) trap and the quadruple Bessel beam (QBB) trap. Under the influence of a constant gas flow (constant external force), the QBB trap is found to be more stable compared with the CPBB trap. By contrast, under pulsed laser excitation with laser pulse durations of nanoseconds (pulsed external force), the type of trap is of minor importance for the droplet stability. It typically needs pulsed laser forces that are several orders of magnitude higher than the optical forces to induce escape of the droplet from the trap. If the droplet strongly absorbs the pulsed laser light, these escape forces can be strongly reduced. The lower stability of absorbing droplets is a result of secondary thermal processes that cause droplet escape.

Stability of aerosol droplets in Bessel beam optical traps under constant and pulsed external forces.

PubMed

David, Grégory; Esat, Kıvanç; Hartweg, Sebastian; Cremer, Johannes; Chasovskikh, Egor; Signorell, Ruth

2015-04-21

We report on the dynamics of aerosol droplets in optical traps under the influence of additional constant and pulsed external forces. Experimental results are compared with simulations of the three-dimensional droplet dynamics for two types of optical traps, the counter-propagating Bessel beam (CPBB) trap and the quadruple Bessel beam (QBB) trap. Under the influence of a constant gas flow (constant external force), the QBB trap is found to be more stable compared with the CPBB trap. By contrast, under pulsed laser excitation with laser pulse durations of nanoseconds (pulsed external force), the type of trap is of minor importance for the droplet stability. It typically needs pulsed laser forces that are several orders of magnitude higher than the optical forces to induce escape of the droplet from the trap. If the droplet strongly absorbs the pulsed laser light, these escape forces can be strongly reduced. The lower stability of absorbing droplets is a result of secondary thermal processes that cause droplet escape.

Real-time observation of the initiation of RNA polymerase II transcription.

PubMed

Fazal, Furqan M; Meng, Cong A; Murakami, Kenji; Kornberg, Roger D; Block, Steven M

2015-09-10

Biochemical and structural studies have shown that the initiation of RNA polymerase II transcription proceeds in the following stages: assembly of the polymerase with general transcription factors and promoter DNA in a 'closed' preinitiation complex (PIC); unwinding of about 15 base pairs of the promoter DNA to form an 'open' complex; scanning downstream to a transcription start site; synthesis of a short transcript, thought to be about 10 nucleotides long; and promoter escape. Here we have assembled a 32-protein, 1.5-megadalton PIC derived from Saccharomyces cerevisiae, and observe subsequent initiation processes in real time with optical tweezers. Contrary to expectation, scanning driven by the transcription factor IIH involved the rapid opening of an extended transcription bubble, averaging 85 base pairs, accompanied by the synthesis of a transcript up to the entire length of the extended bubble, followed by promoter escape. PICs that failed to achieve promoter escape nevertheless formed open complexes and extended bubbles, which collapsed back to closed or open complexes, resulting in repeated futile scanning.

JOWOG 22/2 - Actinide Chemical Technology (July 9-13, 2012)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jackson, Jay M.; Lopez, Jacquelyn C.; Wayne, David M.

2012-07-05

The Plutonium Science and Manufacturing Directorate provides world-class, safe, secure, and reliable special nuclear material research, process development, technology demonstration, and manufacturing capabilities that support the nation's defense, energy, and environmental needs. We safely and efficiently process plutonium, uranium, and other actinide materials to meet national program requirements, while expanding the scientific and engineering basis of nuclear weapons-based manufacturing, and while producing the next generation of nuclear engineers and scientists. Actinide Process Chemistry (NCO-2) safely and efficiently processes plutonium and other actinide compounds to meet the nation's nuclear defense program needs. All of our processing activities are done in amore » world class and highly regulated nuclear facility. NCO-2's plutonium processing activities consist of direct oxide reduction, metal chlorination, americium extraction, and electrorefining. In addition, NCO-2 uses hydrochloric and nitric acid dissolutions for both plutonium processing and reduction of hazardous components in the waste streams. Finally, NCO-2 is a key team member in the processing of plutonium oxide from disassembled pits and the subsequent stabilization of plutonium oxide for safe and stable long-term storage.« less

Risks incurred by hydrogen escaping from containers and conduits

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swain, M.R.; Grilliot, E.S.

1998-08-01

This paper is a discussion of a method for hydrogen leak classification. Leaks are classified as; gas escapes into enclosed spaces, gas escapes into partially enclosed spaces (vented), and gas escapes into unenclosed spaces. Each of the three enclosure classifications is further divided into two subclasses; total volume of hydrogen escaped and flow rate of escaping hydrogen. A method to aid in risk assessment determination in partially enclosed spaces is proposed and verified for several enclosure geometries. Examples are discussed for additional enclosure geometries.

Micronozzles: 3D numerical structural and gas dynamics modeling, fabrication, and preliminary experimental results

NASA Astrophysics Data System (ADS)

Borovkov, Alexei I.; Pyatishev, Evgenij N.; Lurie, Mihail S.; Korshunov, Andrey V.; Akulshin, Y. D.; Dolganov, A. G.; Sabadash, V. O.

2000-02-01

The tiny engines, founded on the principle of reactive thrust, are one of most perspective actuators developed by modern micromechanics. These engines can be applied for such apparent problems, as orientation and stabilization of small space objects, but also as local or distributed reactive thrust of new phylum of aerospace objects, for control of boundary layer of flying objects and in series of converting power devices of different purposes. Distinctive features of jet tiny engines are profitability (very large thrust-to-weight ratio) and high (milliseconds) response, which makes them to irreplaceable elements in control systems and, specially, in distributed power generations. These features are provided the minimum sizes, high pressure in working chambers and hypersonic velocity of propulsive jet. Topologically micronozzles are designed as the flat batch devices (3 layers as minimum). The lower and upper layers make flat walls of the nozzle and mainly influence on strength properties of the device. The mean layer reshapes geometry and determines gas dynamic characteristic of the nozzle. A special problem is the opening-up of the combustion-mixture, which is not esteemed in this work. It is necessary to allow for effect of considerable local stresses arising at the expense of static and dynamic loading at design of the jet tiny engines. Thermal gas dynamic processes in the chamber and nozzle determine the values and nature of these stresses, which are hardly studied for the microdevices. The priority is mathematical and experimental simulation of these processes. The most suitable object for initial phase of experimental simulation is the 'cold' engine. The demanded chamber static pressure is formed by external compressed air. In Laboratory of Microtechnology and MicroElectroMechanical Systems a number of such tiny engines with different shapes of the chamber's and the nozzles' surfaces were designed, made and tested. The engines were produced from photosensing glass by methods of microtechnology on the basis of photolithography processes. After expositing through a mask the latent map of the glass was 'showed' by heat treatment and etched. The obtained parts sitallized and subjected to level-by-level assembly. At experiments on 'ardent' engines it is supposed to keep the basic stages of a technological route, but to use stronger and temperature- resistant materials including coating from high-strength membranes plotted by vacuum deposition methods. During trial tests, for the 'cold' engine with an altitude of a nozzle of 1.2 mm and width of the throat of 0.4 mm at chamber pressure 0.6 MPa the exhaust velocity on escaping of the nozzle about 1.5 M was obtained. The engine thrust has compounded 45 gr. The obtained data are in satisfactory conformity with 1D computation and allow to proceed piloting objects of other range of the characteristics. The microactuators having high response and profitability are demanded for perspective small aerospace objects. This activators are indispensable for creation of distributed thrust and control of boundary layer of micro air flying objects (MAV), for devices of stabilization and orientation of micro-satellites. A number of such activators forms on the areas of flat micronozzle devices. Developed micronozzles should provide demanded parameters at the expense of a high level of pressure in working chamber and supersonic exhaust velocities. At creation of the micronozzle the effect of considerable loads arising as at the expense of static, and dynamic loading should be mentioned. Thermomechanics-gasodynamic processes in the chamber and nozzle determine the nature and kind of loading. Mathematical and experimental simulation of these hardly studied for the microscopic object processes is necessary.

Micronozzles: 3D numerical structural and gas dynamics modeling, fabrication, and preliminary experimental results

NASA Astrophysics Data System (ADS)

Borovkov, Alexei I.; Pyatishev, Evgenij N.; Lurie, Mihail S.; Korshunov, Andrey V.; Akulshin, Y. D.; Dolganov, A. G.; Sabadash, V. O.

2001-02-01

The tiny engines, founded on the principle of reactive thrust, are one of most perspective actuators developed by modern micromechanics. These engines can be applied for such apparent problems, as orientation and stabilization of small space objects, but also as local or distributed reactive thrust of new phylum of aerospace objects, for control of boundary layer of flying objects and in series of converting power devices of different purposes. Distinctive features of jet tiny engines are profitability (very large thrust-to-weight ratio) and high (milliseconds) response, which makes them to irreplaceable elements in control systems and, specially, in distributed power generations. These features are provided the minimum sizes, high pressure in working chambers and hypersonic velocity of propulsive jet. Topologically micronozzles are designed as the flat batch devices (3 layers as minimum). The lower and upper layers make flat walls of the nozzle and mainly influence on strength properties of the device. The mean layer reshapes geometry and determines gas dynamic characteristic of the nozzle. A special problem is the opening-up of the combustion-mixture, which is not esteemed in this work. It is necessary to allow for effect of considerable local stresses arising at the expense of static and dynamic loading at design of the jet tiny engines. Thermal gas dynamic processes in the chamber and nozzle determine the values and nature of these stresses, which are hardly studied for the microdevices. The priority is mathematical and experimental simulation of these processes. The most suitable object for initial phase of experimental simulation is the 'cold' engine. The demanded chamber static pressure is formed by external compressed air. In Laboratory of Microtechnology and MicroElectroMechanical Systems a number of such tiny engines with different shapes of the chamber's and the nozzles' surfaces were designed, made and tested. The engines were produced from photosensing glass by methods of microtechnology on the basis of photolithography processes. After expositing through a mask the latent map of the glass was 'showed' by heat treatment and etched. The obtained parts sitallized and subjected to level-by-level assembly. At experiments on 'ardent' engines it is supposed to keep the basic stages of a technological route, but to use stronger and temperature- resistant materials including coating from high-strength membranes plotted by vacuum deposition methods. During trial tests, for the 'cold' engine with an altitude of a nozzle of 1.2 mm and width of the throat of 0.4 mm at chamber pressure 0.6 MPa the exhaust velocity on escaping of the nozzle about 1.5 M was obtained. The engine thrust has compounded 45 gr. The obtained data are in satisfactory conformity with 1D computation and allow to proceed piloting objects of other range of the characteristics. The microactuators having high response and profitability are demanded for perspective small aerospace objects. This activators are indispensable for creation of distributed thrust and control of boundary layer of micro air flying objects (MAV), for devices of stabilization and orientation of micro-satellites. A number of such activators forms on the areas of flat micronozzle devices. Developed micronozzles should provide demanded parameters at the expense of a high level of pressure in working chamber and supersonic exhaust velocities. At creation of the micronozzle the effect of considerable loads arising as at the expense of static, and dynamic loading should be mentioned. Thermomechanics-gasodynamic processes in the chamber and nozzle determine the nature and kind of loading. Mathematical and experimental simulation of these hardly studied for the microscopic object processes is necessary.

Diffusion-Limited Cargo Loading of an Engineered Protein Container.

PubMed

Zschoche, Reinhard; Hilvert, Donald

2015-12-30

The engineered bacterial nanocompartment AaLS-13 is a promising artificial encapsulation system that exploits electrostatic interactions for cargo loading. In order to study its ability to take up and retain guests, a pair of fluorescent proteins was developed which allows spectroscopic determination of the extent of encapsulation by Förster resonance energy transfer (FRET). The encapsulation process is generally complete within a second, suggesting low energetic barriers for proteins to cross the capsid shell. Formation of intermediate aggregates upon mixing host and guest in vitro complicates capsid loading at low ionic strength, but can be sidestepped by increasing salt concentrations or diluting the components. Encapsulation of guests is completely reversible, and the position of the equilibrium is easily tuned by varying the ionic strength. These results, which challenge the notion that AaLS-13 is a continuous rigid shell, provide valuable information about cargo loading that will guide ongoing efforts to engineer functional host-guest complexes. Moreover, it should be possible to adapt the protein FRET pair described in this report to characterize functional capsid-cargo complexes generated by other encapsulation systems.

Creating Engaging Escape Rooms for the Classroom

ERIC Educational Resources Information Center

Nicholson, Scott

2018-01-01

Escape rooms are "live-action team-based games where players discover clues, solve puzzles, and accomplish tasks in one or more rooms in order to accomplish a specific goal (usually escaping from the room) in a limited amount of time." Escape Rooms are one type of Escape Game, which are narrative-based challenges that use puzzles, tasks,…

To hear or not to hear: Voice processing under visual load.

PubMed

Zäske, Romi; Perlich, Marie-Christin; Schweinberger, Stefan R

2016-07-01

Adaptation to female voices causes subsequent voices to be perceived as more male, and vice versa. This contrastive aftereffect disappears under spatial inattention to adaptors, suggesting that voices are not encoded automatically. According to Lavie, Hirst, de Fockert, and Viding (2004), the processing of task-irrelevant stimuli during selective attention depends on perceptual resources and working memory. Possibly due to their social significance, faces may be an exceptional domain: That is, task-irrelevant faces can escape perceptual load effects. Here we tested voice processing, to study whether voice gender aftereffects (VGAEs) depend on low or high perceptual (Exp. 1) or working memory (Exp. 2) load in a relevant visual task. Participants adapted to irrelevant voices while either searching digit displays for a target (Exp. 1) or recognizing studied digits (Exp. 2). We found that the VGAE was unaffected by perceptual load, indicating that task-irrelevant voices, like faces, can also escape perceptual-load effects. Intriguingly, the VGAE was increased under high memory load. Therefore, visual working memory load, but not general perceptual load, determines the processing of task-irrelevant voices.

The Deuterium to Hydrogen Ratio in the Water that Formed the Yellowknife Bay Mudstones in Gale Crater

NASA Technical Reports Server (NTRS)

Mahaffy, Paul R.; Brunner, Anna E.; Webster, Chris R.; Atreya, Sushil K.; Mcadam, Amy Catherine; Stern, Jennifer Claire; Leshin, Laurie Ann; Navarro-Gonzales, Rafael; Jones, J.; Kashyap, Srishti

2014-01-01

A suite of isotope ratios of light elements in the present martian atmosphere (13C/12C, 15N/14N, 18O/16O, 38Ar/36Ar, and D/H) are all substantially enriched in the heavy element suggesting atmospheric loss to space over the past billions of years with preferential loss of the lighter isotope from each pair. In situ measurements from MSL's Sample Analysis at Mars (SAM) instrument [e.g. 1,2,3] have considerably refined previous measurements from the Viking mass spectrometers [e.g. 4], from remote spectroscopic observations [e.g. 5,6], and from martian meteorite studies [e.g. 7,8]. The persistence of habitable environments such as the ancient Yellowknife Bay lake recently revealed by measurements from the Curiosity rover [9] depends on the surface temperatures and the duration of an atmosphere thicker than that at present. Current and planned measurements from orbit with the Mars Express and MAVEN missions respectively intend to study the processes of atmospheric escape including solar wind interaction, sputtering, thermal escape, and dissociative recombination, and determine or refine the current rate of atmospheric loss caused by these and other mechanisms. The goal of these programs is to understand the physical processes sufficiently well so that robust extrapolations over the past billions of years can be made D/H is measured by both the Tunable Laser Spectrometer (TLS) and the Quadrupole Mass Spectrometer (QMS) of the SAM suite. to predict the atmospheric and surface conditions on early Mars. However, the study of the history of martian atmospheric evolution will be greatly facilitated if we are able to also directly measure the isotopic composition of volatiles captured in rocks that are representative of the ancient atmosphere. To date, D/H is one of the most promising candidates for this study since water is the most abundant volatile thermally released from the Yellowknife Bay phylosilicates discovered by the SAM and CheMin experiments of MSL and its

Extreme values and the level-crossing problem: An application to the Feller process

NASA Astrophysics Data System (ADS)

Masoliver, Jaume

2014-04-01

We review the question of the extreme values attained by a random process. We relate it to level crossings to one boundary (first-passage problems) as well as to two boundaries (escape problems). The extremes studied are the maximum, the minimum, the maximum absolute value, and the range or span. We specialize in diffusion processes and present detailed results for the Wiener and Feller processes.

On Mars' Atmospheric Sputtering after Maven First two Years

NASA Astrophysics Data System (ADS)

Leblanc, F.; Modolo, R.; Curry, S.; Luhmann, J. G.; Lillis, R. J.; Chaufray, J. Y.; Hara, T.; McFadden, J. P.; Halekas, J. S.; Epavier, F.; Connerney, J. E. P.; Jakosky, B. M.

2017-12-01

Mars likely lost a significant part of its atmosphere to space during its history. The sputtering of the atmosphere by precipitating planetary picked up ions accelerated by the solar wind is one of the processes that could have significantly contributed to this atmospheric escape, in particular since the cessation of its global magnetic field, 4.0-4.1 Gyr ago. We here present a two year baseline analysis of MAVEN observations of the precipitating flux, in particular the dependence of the precipitating flux on solar zenith angle. We use this measurement to model the expected escape rate and exospheric structure induced by this precipitation.

MAVEN observations of the Mars upper atmosphere, ionosphere, and solar wind interactions

NASA Astrophysics Data System (ADS)

Jakosky, Bruce M.

2017-09-01

The Mars Atmosphere and Volatile Evolution (MAVEN) mission to Mars has been operating in orbit for more than a full Martian year. Observations are dramatically changing our view of the Mars upper atmosphere system, which includes the upper atmosphere, ionosphere, coupling to the lower atmosphere, magnetosphere, and interactions with the Sun and the solar wind. The data are allowing us to understand the processes controlling the present-day structure of the upper atmosphere and the rates of escape of gas to space. These will tell us the role that escape to space has played in the evolution of the Mars atmosphere and climate.

Variational transition state theory for multidimensional activated rate processes in the presence of anisotropic friction

NASA Astrophysics Data System (ADS)

Berezhkovskii, Alexander M.; Frishman, Anatoli M.; Pollak, Eli

1994-09-01

Variational transition state theory (VTST) is applied to the study of the activated escape of a particle trapped in a multidimensional potential well and coupled to a heat bath. Special attention is given to the dependence of the rate constant on the friction coefficients in the case of anisotropic friction. It is demonstrated explicitly that both the traditional as well as the nontraditional scenarios for the particle escape are recovered uniformly within the framework of VTST. Effects such as saddle point avoidance and friction dependence of the activation energy are derived from VTST using optimized planar dividing surfaces.

A comparison of positive and negative reinforcement for compliance to treat problem behavior maintained by escape.

PubMed

Slocum, Sarah K; Vollmer, Timothy R

2015-09-01

Previous research has shown that problem behavior maintained by escape can be treated using positive reinforcement. In the current study, we directly compared functional (escape) and nonfunctional (edible) reinforcers in the treatment of escape-maintained problem behavior for 5 subjects. In the first treatment, compliance produced a break from instructions. In the second treatment, compliance produced a small edible item. Neither treatment included escape extinction. Results suggested that the delivery of a positive reinforcer for compliance was effective for treating escape-maintained problem behavior for all 5 subjects, and the delivery of escape for compliance was ineffective for 3 of the 5 subjects. Implications and future directions related to the use of positive reinforcers in the treatment of escape behavior are discussed. © Society for the Experimental Analysis of Behavior.

An empirical investigation of time-out with and without escape extinction to treat escape-maintained noncompliance.

PubMed

Everett, Gregory E; Joe Olmi, D; Edwards, Ron P; Tingstrom, Daniel H; Sterling-Turner, Heather E; Christ, Theodore J

2007-07-01

The present study evaluates the effectiveness of two time-out (TO) procedures in reducing escape-maintained noncompliance of 4 children. Noncompliant behavioral function was established via a functional assessment (FA), including indirect and direct descriptive procedures and brief confirmatory experimental analyses. Following FA, parents were taught to consequate noncompliance with two different TO procedures, one without and one with escape extinction following TO release. Although results indicate TO without escape extinction is effective in increasing compliance above baseline levels, more optimal levels of compliance were obtained for all 4 children when escape extinction was added to the TO procedures already in place. Results indicate efficacy of TO with escape extinction when applied to escape-maintained noncompliance and are discussed as an initial example of the successful application of TO to behaviors maintained by negative reinforcement.

Controls on methane expulsion during melting of natural gas hydrate systems. Topic area 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Flemings, Peter

1.1. Project Goal The project goal is to predict, given characteristic climate-induced temperature change scenarios, the conditions under which gas will be expelled from existing accumulations of gas hydrate into the shallow ocean or directly to the atmosphere. When those conditions are met, the fraction of the gas accumulation that escapes and the rate of escape shall be quantified. The predictions shall be applicable in Arctic regions and in gas hydrate systems at the up dip limit of the stability zone on continental margins. The behavior shall be explored in response to two warming scenarios: longer term change due tomore » sea level rise (e.g. 20 thousand years) and shorter term due to atmospheric warming by anthropogenic forcing (decadal time scale). 1.2. Project Objectives During the first budget period, the objectives are to review and categorize the stability state of existing well-studied hydrate reservoirs, develop conceptual and numerical models of the melting process, and to design and conduct laboratory experiments that dissociate methane hydrate in a model sediment column by systematically controlling the temperature profile along the column. The final objective of the first budget period shall be to validate the models against the experiments. In the second budget period, the objectives are to develop a model of gas flow into sediment in which hydrate is thermodynamically stable, and conduct laboratory experiments of this process to validate the model. The developed models shall be used to quantify the rate and volume of gas that escapes from dissociating hydrate accumulations. In addition, specific scaled simulations characteristic of Arctic regions and regions near the stability limit at continental margins shall be performed. 1.3. Project Background and Rationale The central hypothesis proposed is that hydrate melting (dissociation) due to climate change generates free gas that can, under certain conditions, propagate through the gas hydrate stability zone and vent at the seafloor. Gas venting through the regional hydrate stability zone is accomplished by alteration of the regional equilibrium conditions (creation of three phase conditions) by increased salinity and heat due to hydrate formation, due to gas fracturing, or a combination of both. This research will explore the controls on whether methane reaches the seafloor (or atmosphere) as the original hydrate deposit dissociates and what the magnitude of these fluxes are. This hypothesis has significant implications for the forcings and feedbacks associated with climate change. It is described below the observations and models that have led to formulating this hypothesis.« less

Experimental early-stage coalification of a peat sample and a peatified wood sample from Indonesia

USGS Publications Warehouse

Orem, W.H.; Neuzil, S.G.; Lerch, H.E.; Cecil, C.B.

1996-01-01

Experimental coalification of a peat sample and a buried wood sample from domed peat deposits in Indonesia was carried out to examine chemical structural changes in organic matter during early-stage coalification. The experiment (125 C, 408 atm lithostatic pressure, and 177 atm fluid pressure for 75 days) was designed to maintain both lithostatic and fluid pressure on the sample, but allow by-products that may retard coalification to escape. We refer to this design as a geologically open system. Changes in the elemental composition, and 13C NMR and FTIR spectra of the peat and wood after experimental coalification suggest preferential thermal decomposition of O-containing aliphatic organic compounds (probably cellulose) during early-stage coalification. The elemental compositions and 13C NMR spectra of the experimentally coalified peat and wood were generally similar to those of Miocene coal and coalified wood samples from Indonesia. Yields of lignin phenols in the peat and wood samples decreased following experimental coalification; the wood sample exhibited a larger change. Lignin phenol yields from the experimentally coalified peat and wood were comparable to yields of lignin phenols from Miocene Indonesian lignite and coalified wood. Changes in syringyl/vanillyl and p-hydroxy/vanillyl ratios suggest direct demethoxylation as a secondary process to demethylation of methoxyl groups during early coalification, and changes in lignin phenol yields and acid/aldehyde ratios point to a coupling between demethoxylation processes and reactions in the alkyl side chain bonds of the ??-carbon in lignin phenols.

Loss of Water to Space from Mars: Processes and Implications

NASA Astrophysics Data System (ADS)

Kass, D. M.

2001-12-01

One of the major sinks for water on Mars is the loss to space. This occurs via a complex series of processes that transport the individual atoms to the upper atmosphere, where several escape mechanisms remove them. Hydrogen and deuterium are lost primarily by Jeans escape. Non-thermal processes also remove H and D, but are only important in determining D loss at solar minimum under modern conditions. The present H loss rate is equivalent to the loss of 10-3~pr-\\micron~yr-1 of water. The loss of oxygen is more complicated. The three main processes are indirect (or ionospheric) sputtering, solar wind pickup of O+, and O2+ dissociative recombination. Their relative importance has varied over the history of Mars. The combined effect of the O loss processes is to remove a ~ 50~m global layer of water over the last 3.5 Gyr. Based on photochemical modeling, the loss of oxygen and hydrogen are balanced (over geological timescales) by a feedback process. During the early history of Mars, impact erosion and hydrodynamic blow-off may have removed significant water. But, it is difficult to estimate their quantitative effects. The transport of individual H, D and O atoms to the exosphere where they can escape is not completely understood. It occurs primarily via intermediate species, H2, HD, O2 and CO2. The H2 and HD are formed by photolysis of water and the odd hydrogen photochemistry. One open issue is the mechanism regulating the partitioning of D between HDO and HD (which controls the supply of D available for escape from the exosphere). The various loss processes isotopically enrich Martian water since the exospheric escape source region is depleted. Jeans escape and the transport from the lower atmosphere further fractionate hydrogen, the most useful isotopic system. Based on recent observations, the D/H fractionation factor, F ~ 0.02. Measurements of atmospheric water vapor indicate it is enriched in deuterium, with a D/H ratio ~ 5 times the terrestrial value. Since most of the water on Mars is likely to be in the form of ice, it is presumably further fractionated by ~ 0.8 due to ice/water vapor interactions. This yields an effective D/H enrichment of ~ 7 for reservoirs in equilibrium with the atmosphere. From a loss to space point of view, Martian water can be divided into three reservoirs. The first is the thin, 10 pr-\\micron, atmospheric water. The second is a global exchangeable reservoir in long term isotopic equilibrium with the atmosphere. This probably encompasses the polar caps, ice in polar layered deposits and any other near surface ice or adsorbed water. The third, more speculative, reservoir is a non-exchanging reservoir (a deep sub-surface cryosphere). In addition, due to the small size of the atmospheric reservoir, difficulty in isotopically equilibrating it with the entire exchangeable reservoir, and the relatively rapid H2 loss rate, there is also an intermediate exchangeable reservoir of ~ 4~mm. This is probably either a surface layer on the polar caps or near surface ice deposits. By assuming an initial terrestrial D/H ratio for Martian water (based on condritic meteorites) and a loss to space of ~ 50~m (based on the total O loss), the size of the exchangeable reservoir can be estimated. Two conceptual models are possible, depending on whether or not the non-exchangeable reservoir replenishes the exchangeable one. Quantitatively, the two models yield almost identical reservoir sizes, ~ 9~m (about the size of the northern polar cap). If, due to slow rate of isotopic diffusion in ice, the exchangeable reservoir actually has the same isotopic enrichment as the atmosphere, it would contain ~ 12~m of water.

In-Flight Operation of the Dawn Ion Propulsion System - The First Nine Months

NASA Technical Reports Server (NTRS)

Garner, Charles E.; Brophy, John R.; Mikes, Steven C.; Raymond, Marc D.

2008-01-01

The Dawn mission, part of NASA's Discovery Program, has as its goal the scientific exploration of the two most massive main-belt asteroids, Vesta and Ceres. The Dawn spacecraft was launched from Cape Canaveral Air Force Station on September 27, 2007 on a Delta-II 7925H-9.5 (Delta-II Heavy) rocket that placed the 1218 kg spacecraft into an Earth-escape trajectory. On-board the spacecraft is an ion propulsion system (IPS) which will provide most of the delta-V needed for heliocentric transfer to Vesta, orbit capture at Vesta, transfer to Vesta science orbits, departure and escape from Vesta, heliocentric transfer to Ceres, orbit capture at Ceres, and transfer to Ceres science orbits. The Dawn ion engine design is based on the design validated on NASA's Deep Space 1 mission. However, because of the very substantial (11 km/s) delta-V requirements for this mission Dawn requires two engines to complete its mission objectives. The power processor units (PPU), digital control and interface units (DCIU) slice boards and the xenon control assembly (XCA) are also based on the DS1 design. The DCIUs and thrust gimbal assemblies (TGA) were developed at the Jet Propulsion Laboratory. The spacecraft was provided by Orbital Sciences Corporation, Sterling, Virginia, and the mission is managed by and operated from the Jet Propulsion Laboratory. Dawn partnered with Germany, Italy and Los Alamos National Laboratory for the science instruments. The mission is led by the principal investigator, Dr. Christopher Russell, from the University of California, Los Angeles. The first 80 days after launch were dedicated to the initial checkout of the spacecraft prior to the initiation of long-term thrusting for the heliocentric transfer to Vesta. The IPS hardware, consisting of three ion thrusters and TGAs, two PPUs and DCIUs, xenon feed system, and spacecraft control software, was investigated extensively. Thrust measurements, roll torque measurements, pointing capabilities, control characteristics, and thermal behavior of the spacecraft and IPS were carefully evaluated. The Dawn IPS fully met all its initial checkout performance objectives. Deterministic thrusting for cruise began on December 17, 2007. Over the subsequent approximately 330 days the IPS will be operated virtually continuously at full power thrusting (approximately 91 mN) leading to a Mars flyby in February 2009. The encounter with Mars provides a gravity assist for a plane change and is the only source of post-launch delta-V apart from the IPS. Following the Mars gravity assist IPS will be operated for approximately one year at full power and for 1.3 years at throttled power levels leading to rendezvous with Vesta in August of 2011. Following nine months of orbital operations with IPS providing the propulsion needed for orbit capture, science orbit transfer and orbit maintenance and Vesta escape, Dawn will transit to Ceres with an expected arrival date of February 2015. As of June 16, 2008 the ion thrusters on Dawn have operated for close to 3,846 hours and have delivered nearly 1 km/s of delta-V to the spacecraft. Dawn IPS operation has been almost flawless during the initial checkout and six months of cruise. This paper provides an overview of Dawn's mission objectives, mission and system design, and the results of the post-launch Dawn IPS mission operations through June 2008

Frequent and Variable Cytotoxic-T-Lymphocyte Escape-Associated Fitness Costs in the Human Immunodeficiency Virus Type 1 Subtype B Gag Proteins

PubMed Central

Boutwell, Christian L.; Carlson, Jonathan M.; Lin, Tien-Ho; Seese, Aaron; Power, Karen A.; Peng, Jian; Tang, Yanhua; Brumme, Zabrina L.; Heckerman, David; Schneidewind, Arne

2013-01-01

Cytotoxic-T-lymphocyte (CTL) escape mutations undermine the durability of effective human immunodeficiency virus type 1 (HIV-1)-specific CD8+ T cell responses. The rate of CTL escape from a given response is largely governed by the net of all escape-associated viral fitness costs and benefits. The observation that CTL escape mutations can carry an associated fitness cost in terms of reduced virus replication capacity (RC) suggests a fitness cost-benefit trade-off that could delay CTL escape and thereby prolong CD8 response effectiveness. However, our understanding of this potential fitness trade-off is limited by the small number of CTL escape mutations for which a fitness cost has been quantified. Here, we quantified the fitness cost of the 29 most common HIV-1B Gag CTL escape mutations using an in vitro RC assay. The majority (20/29) of mutations reduced RC by more than the benchmark M184V antiretroviral drug resistance mutation, with impacts ranging from 8% to 69%. Notably, the reduction in RC was significantly greater for CTL escape mutations associated with protective HLA class I alleles than for those associated with nonprotective alleles. To speed the future evaluation of CTL escape costs, we also developed an in silico approach for inferring the relative impact of a mutation on RC based on its computed impact on protein thermodynamic stability. These data illustrate that the magnitude of CTL escape-associated fitness costs, and thus the barrier to CTL escape, varies widely even in the conserved Gag proteins and suggest that differential escape costs may contribute to the relative efficacy of CD8 responses. PMID:23365420

Carbon isotopic characterization of formaldehyde emitted by vehicles in Guangzhou, China

NASA Astrophysics Data System (ADS)

Hu, Ping; Wen, Sheng; Liu, Yonglin; Bi, Xinhui; Chan, Lo Yin; Feng, Jialiang; Wang, Xinming; Sheng, Guoying; Fu, Jiamo

2014-04-01

Formaldehyde (HCHO) is the most abundant carbonyl compound in the atmosphere, and vehicle exhaust emission is one of its important anthropogenic sources. However, there is still uncertainty regarding HCHO flux from vehicle emission as well as from other sources. Herein, automobile source was characterized using HCHO carbon isotopic ratio to assess its contributions to atmospheric flux and demonstrate the complex production/consumption processes during combustion in engine cylinder and subsequent catalytic treatment of exhaust. Vehicle exhausts were sampled under different idling states and HCHO carbon isotopic ratios were measured by gas chromatograph-combustion-isotopic ratio mass spectrometry (GC-C-IRMS). The HCHO directly emitted from stand-alone engines (gasoline and diesel) running at different load was also sampled and measured. The HCHO carbon isotopic ratios were from -30.8 to -25.7‰ for gasoline engine, and from -26.2 to -20.7‰ for diesel engine, respectively. For diesel vehicle without catalytic converter, the HCHO carbon isotopic ratios were -22.1 ± 2.1‰, and for gasoline vehicle with catalytic converter, the ratios were -21.4 ± 0.7‰. Most of the HCHO carbon isotopic ratios were heavier than the fuel isotopic ratios (from -29 to -27‰). For gasoline vehicle, the isotopic fractionation (Δ13C) between HCHO and fuel isotopic ratios was 7.4 ± 0.7‰, which was higher than that of HCHO from stand-alone gasoline engine (Δ13Cmax = 2.7‰), suggesting additional consumption by the catalytic converter. For diesel vehicle without catalytic converter, Δ13C was 5.7 ± 2.0‰, similar to that of stand-alone diesel engine. In general, the carbon isotopic signatures of HCHO emitted from automobiles were not sensitive to idling states or to other vehicle parameters in our study condition. On comparing these HCHO carbon isotopic data with those of past studies, the atmospheric HCHO in a bus station in Guangzhou might mainly come from vehicle emission for the accordance of carbon isotopic data.

Solidification in direct metal deposition by LENS processing

NASA Astrophysics Data System (ADS)

Hofmeister, William; Griffith, Michelle

2001-09-01

Thermal imaging and metallographic analysis were used to study Laser Engineered Net Shaping (LENS™) processing of 316 stainless steel and H13 tool steel. The cooling rates at the solid-liquid interface were measured over a range of conduction conditions. The length scale of the molten zone controls cooling rates during solidification in direct metal deposition. In LENS processing, the molten zone ranges from 0.5 mm in length to 1.5 mm, resulting in cooling rates at the solid-liquid interface ranging from 200 6,000 Ks-1.

Improving The Prototyping Process In Department Of Defense Acquisition

DTIC Science & Technology

2014-06-01

System Flow Chart ................................................. 39 Figure 13. TRL Definitions (from ASD [R&E] 2011... ASD (R&E) Assistant Secretary of Defense for Research and Engineering BCL Business Capability Life cycle CDD Capability Development Document CDR...TRL 6 cannot be attained until the technology has been demonstrated in a relevant operational environment ( ASD [R&E] 2011). A technology that has

40 CFR Appendix C-1 to Subpart E... - Required Provisions-Consulting Engineering Agreements

Code of Federal Regulations, 2010 CFR

2010-07-01

.... Changes 5. Termination 6. Remedies 7. Payment 8. Project Design 9. Audit; Access to Records 10. Price... production techniques, methods, and processes, consistent with 40 CFR 35.936-3 and 35.936-13 in effect on the date of execution of this agreement, except to the extent to which innovative technology may be used...

Photochemical escape of oxygen from Mars: constraints from MAVEN in situ measurements

NASA Astrophysics Data System (ADS)

Lillis, R. J.; Deighan, J.; Fox, J. L.; Bougher, S. W.; Lee, Y.; Cravens, T.; Rahmati, A.; Mahaffy, P. R.; Andersson, L.; Combi, M. R.; Benna, M.; Jakosky, B. M.; Gröller, H.

2016-12-01

One of the primary goals of the MAVEN mission is to characterize rates of atmospheric escape from Mars at the present epoch and relate those escape rates to solar drivers. Photochemical escape of oxygen is expected to be a significant channel for atmospheric loss, particularly in the early solar system when extreme ultraviolet (EUV) fluxes were much higher. We use near-periapsis (<400 km altitude) data from three instruments. The Langmuir Probe and Waves (LPW) instrument measures electron density and temperature, the Suprathermal And Thermal Ion Composition (STATIC) experiment measures ion temperature and the Neutral Gas and Ion Mass Spectrometer (NGIMS) measures neutral and ion densities. For each profile of in situ measurements, we make a series of calculations, each as a function of altitude. The first uses electron and ion temperatures to calculate the probability distribution for initial energies of hot O atoms. The second calculates the probability that a hot atom born at that altitude will escape. The third takes calculates the production rate of the hot O atoms. We then multiply together the profiles of hot atom production and escape probability to get profiles of the production rate of escaping atoms. We integrate with respect to altitude to give us the escape flux of hot oxygen atoms for that periapsis pass. We will present escape fluxes and derived escape rates from the first Mars year of data collected. Total photochemical loss over time is not very useful to calculate from such escape fluxes derived from current conditions because a thicker atmosphere and much higher solar EUV in the past may change the dynamics of escape dramatically. In the future, we intend to use 3-D Monte Carlo models of global atmospheric escape, in concert with our in situ and remote measurements, to fully characterize photochemical escape under current conditions and carefully extrapolate back in time using further simulations with new boundary conditions.

A Combined Hazard Index Fire Test Methodology for Aircraft Cabin Materials. Volume I.

DTIC Science & Technology

1982-04-01

PROGRAM TEST PANEL NO. 1 ....... 52 5 SUMARY OF EXPERIMTAL CHAS/SATS DATA FOR CI PRGRAM TEST PANEL 2, 3 & 4...As indicated in Figure 2, the dose of each hazard building up in CHI zone 13 is approaching an "effective dose" limit which prevents occupant escape...per minute. During a test, flow into SATS was stopped when CO reached peak concentrations to prevent dilution thereafter at decreasing sample CO

13C-based metabolic flux analysis: fundamentals and practice.

PubMed

Yang, Tae Hoon

2013-01-01

Isotope-based metabolic flux analysis is one of the emerging technologies applied to system level metabolic phenotype characterization in metabolic engineering. Among the developed approaches, (13)C-based metabolic flux analysis has been established as a standard tool and has been widely applied to quantitative pathway characterization of diverse biological systems. To implement (13)C-based metabolic flux analysis in practice, comprehending the underlying mathematical and computational modeling fundamentals is of importance along with carefully conducted experiments and analytical measurements. Such knowledge is also crucial when designing (13)C-labeling experiments and properly acquiring key data sets essential for in vivo flux analysis implementation. In this regard, the modeling fundamentals of (13)C-labeling systems and analytical data processing are the main topics we will deal with in this chapter. Along with this, the relevant numerical optimization techniques are addressed to help implementation of the entire computational procedures aiming at (13)C-based metabolic flux analysis in vivo.

13. Historic drawing of rocket engine test facility layout, including ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Historic drawing of rocket engine test facility layout, including Buildings 202, 205, 206, and 206A, February 3, 1984. NASA GRC drawing number CF-101539. On file at NASA Glenn Research Center. - Rocket Engine Testing Facility, NASA Glenn Research Center, Cleveland, Cuyahoga County, OH

Study of basic physical processes in liquid rocket engines

NASA Technical Reports Server (NTRS)

Wu, S. T.; Chen, C. P.

1992-01-01

Inconsistencies between analytical results and measurements for liquid rocket thrust chamber performance, which escape suitable explanations, have motivated the examination of the basic phys ical modeling formulations as to their unlimited application. The publication of Prof. D. Straub's book, 'Thermofluid-dynamics of Optimized Rocket Propulsions,' further stimulated the interest of understanding the gas dynamic relationships in chemically reacting mixtures. A review of other concepts proposed by Falk-Ruppel (Gibbsian Thermodynamics), Straub (Alternative Theory, AT), Prigogine (Non-Equilibrium Thermodynamics), Boltzmann (Kinetic Theory), and Truesdell (Rational Mechanism) has been made to obtain a better understanding of the Navier-Stokes equation, which is now used extensively for chemically reacting flow treatment in combustion chambers. In addition to the study of the different concepts, two workshops were conducted to clarify some of the issues. The first workshop centered on Falk-Ruppel's new 'dynamics' concept, while the second one concentrated on Straub's AT. In this report brief summaries of the reviewed philosophies are presented and compared with the classical Navier-Stokes formulation in a tabular arrangement. Also the highlights of both workshops are addressed.

The Deployment of a Commercial RGA to the International Space Station

NASA Technical Reports Server (NTRS)

Kowitt, Matt; Hawk, Doug; Rossetti, Dino; Woronowicz, Michael

2015-01-01

The International Space Station (ISS) uses ammonia as a medium for heat transport in its Active Thermal Control System. Over time, there have been intermittent component failures and leaks in the ammonia cooling loop. One specific challenge in dealing with an ammonia leak on the exterior of the ISS is determining the exact location from which ammonia is escaping before addressing the problem. Together, researchers and engineers from Stanford Research Systems (SRS) and NASA's Johnson Space Center and Goddard Space Flight Center have adapted a commercial off-the-shelf (COTS) residual gas analyzer (RGA) for repackaging and operation outside the ISS as a core component in the ISS Robotic External Leak Locator, a technology demonstration payload currently scheduled for launch during 2015. The packaging and adaptation of the COTS RGA to the Leak Locator will be discussed. The collaborative process of adapting a commercial instrument for spaceflight will also be reviewed, including the build-­-up of the flight units. Measurements from a full-­-scale thermal vacuum test will also be presented demonstrating the absolute and directional sensitivity of the RGA.

Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway.

PubMed

Kildegaard, Kanchana R; Jensen, Niels B; Schneider, Konstantin; Czarnotta, Eik; Özdemir, Emre; Klein, Tobias; Maury, Jérôme; Ebert, Birgitta E; Christensen, Hanne B; Chen, Yun; Kim, Il-Kwon; Herrgård, Markus J; Blank, Lars M; Forster, Jochen; Nielsen, Jens; Borodina, Irina

2016-03-15

In the future, oil- and gas-derived polymers may be replaced with bio-based polymers, produced from renewable feedstocks using engineered cell factories. Acrylic acid and acrylic esters with an estimated world annual production of approximately 6 million tons by 2017 can be derived from 3-hydroxypropionic acid (3HP), which can be produced by microbial fermentation. For an economically viable process 3HP must be produced at high titer, rate and yield and preferably at low pH to minimize downstream processing costs. Here we describe the metabolic engineering of baker's yeast Saccharomyces cerevisiae for biosynthesis of 3HP via a malonyl-CoA reductase (MCR)-dependent pathway. Integration of multiple copies of MCR from Chloroflexus aurantiacus and of phosphorylation-deficient acetyl-CoA carboxylase ACC1 genes into the genome of yeast increased 3HP titer fivefold in comparison with single integration. Furthermore we optimized the supply of acetyl-CoA by overexpressing native pyruvate decarboxylase PDC1, aldehyde dehydrogenase ALD6, and acetyl-CoA synthase from Salmonella enterica SEacs (L641P). Finally we engineered the cofactor specificity of the glyceraldehyde-3-phosphate dehydrogenase to increase the intracellular production of NADPH at the expense of NADH and thus improve 3HP production and reduce formation of glycerol as by-product. The final strain produced 9.8 ± 0.4 g L(-1) 3HP with a yield of 13% C-mol C-mol(-1) glucose after 100 h in carbon-limited fed-batch cultivation at pH 5. The 3HP-producing strain was characterized by (13)C metabolic flux analysis and by transcriptome analysis, which revealed some unexpected consequences of the undertaken metabolic engineering strategy, and based on this data, future metabolic engineering directions are proposed. In this study, S. cerevisiae was engineered for high-level production of 3HP by increasing the copy numbers of biosynthetic genes and improving flux towards precursors and redox cofactors. This strain represents a good platform for further optimization of 3HP production and hence an important step towards potential commercial bio-based production of 3HP.

The Secrets of Scheherazade: Toward a Functional Analysis of Imaginative Literature

PubMed Central

Grant, Lyle K

2005-01-01

A functional analysis of selected aspects of imaginative literature is presented. Reading imaginative literature is described as a process in which the reader makes indirect contact with the contingencies operating on the behavior of story characters. A functional story grammar is proposed in which the reader's experience with a story is interpreted in terms of escape contingencies in which the author initially introduces an establishing operation consisting of a source of tension, which is resolved in some way by the outcome of the story. Although escape contingencies represent the functional basis for the structure of stories, they are to be understood in a context of many other reinforcers for reading fiction. Other contingencies that maintain reading are discussed. Functional analyses of imaginative literature have much to offer, both in improving literary education and in understanding the behavioral processes that occur on the part of the reader. PMID:22477324

KENNEDY SPACE CENTER, FLA. -- From left, NASA Deputy Associate Administrator for Space Station and Shuttle Programs Michael Kostelnik and NASA Space Shuttle Program Manager William Parsons each don an Emergency Life Support Apparatus (ELSA) during training on the proper use of the escape devices. NASA and United Space Alliance (USA) Space Shuttle program management are participating in a leadership workday. The day is intended to provide management with an in-depth, hands-on look at Shuttle processing activities at KSC.

NASA Image and Video Library

2003-12-19

KENNEDY SPACE CENTER, FLA. -- From left, NASA Deputy Associate Administrator for Space Station and Shuttle Programs Michael Kostelnik and NASA Space Shuttle Program Manager William Parsons each don an Emergency Life Support Apparatus (ELSA) during training on the proper use of the escape devices. NASA and United Space Alliance (USA) Space Shuttle program management are participating in a leadership workday. The day is intended to provide management with an in-depth, hands-on look at Shuttle processing activities at KSC.

Fungal Strategies to Evade the Host Immune Recognition.

PubMed

Hernández-Chávez, Marco J; Pérez-García, Luis A; Niño-Vega, Gustavo A; Mora-Montes, Héctor M

2017-09-23

The recognition of fungal cells by the host immune system is key during the establishment of a protective anti-fungal response. Even though the immune system has evolved a vast number of processes to control these organisms, they have developed strategies to fight back, avoiding the proper recognition by immune components and thus interfering with the host protective mechanisms. Therefore, the strategies to evade the immune system are as important as the virulence factors and attributes that damage the host tissues and cells. Here, we performed a thorough revision of the main fungal tactics to escape from the host immunosurveillance processes. These include the composition and organization of the cell wall, the fungal capsule, the formation of titan cells, biofilms, and asteroid bodies; the ability to undergo dimorphism; and the escape from nutritional immunity, extracellular traps, phagocytosis, and the action of humoral immune effectors.

Material development for fan blade containment casing

NASA Astrophysics Data System (ADS)

McMillan, A.

2008-03-01

This paper describes the physics reasoning and the engineering development process for the structured material system adopted for the containment system of the Trent 900 engine. This is the Rolls-Royce engine that powers the Airbus A380 double-decker aeroplane, which is on the point of entering service. The fan blade containment casing is the near cylindrical casing that surrounds the fan blades at the front of the engine. The fan blades provide the main part of the thrust of the engine; the power to the fan is provided through a shaft from the turbine. The fan is approximately three meters in diameter, with the tips of the blade travelling at a little over Mach speed. The purpose of the containment system is to catch and contain a blade in the extremely unlikely event of a part or whole blade becoming detached. This is known as a ''Fan Blade Off (FBO)'' event. The requirement is that no high-energy fragments should escape the containment system; this is essential to prevent damage to other engines or to the fuselage of the aircraft. Traditionally the containment system philosophy has been to provide a sufficiently thick solid metallic skin that the blade cannot penetrate. Obviously, this is heavy. A good choice of metal in this case is a highly ductile steel, which arrests the kinetic energy of the blade through plastic deformation, and possibly, a controlled amount of cracking. This is known as ''hard wall'' containment. More recently, to reduce weight, containment systems have incorporated a Kevlar fibre wrap. In this case, the thinner metallic wall provides some containment, which is backed up by the stretching of the Kevlar fibres. This is known as ''soft wall'' containment; but it suffers the disadvantage of requiring a large empty volume in the nacelle in to which to expand. For the Trent 900 engine, there was a requirement to make a substantial weight saving while still adopting a hard wall style of containment system. To achieve this, a hollow structured material system was developed, with much of the kinetic energy arrest provided by the mechanism of crushing. A number of structural elements were included within the containment system to maximise the area of material involved in the arrest and thereby minimise the overall weight.

Effects of Visual Information on Wind-Evoked Escape Behavior of the Cricket, Gryllus bimaculatus.

PubMed

Kanou, Masamichi; Matsuyama, Akane; Takuwa, Hiroyuki

2014-09-01

We investigated the effects of visual information on wind-evoked escape behavior in the cricket, Gryllus bimaculatus. Most agitated crickets were found to retreat into a shelter made of cardboard installed in the test arena within a short time. As this behavior was thought to be a type of escape, we confirmed how a visual image of a shelter affected wind-evoked escape behavior. Irrespective of the brightness of the visual background (black or white) or the absence or presence of a shelter, escape jumps were oriented almost 180° opposite to the source of the air puff stimulus. Therefore, the direction of wind-evoked escape depends solely depended on the direction of the stimulus air puff. In contrast, the turning direction of the crickets during the escape was affected by the position of the visual image of the shelter. During the wind-evoked escape jump, most crickets turned in the direction in which a shelter was presented. This behavioral nature is presumably necessary for crickets to retreat into a shelter within a short time after their escape jump.

Space Shuttle redesign status

NASA Technical Reports Server (NTRS)

Brand, Vance D.

1986-01-01

NASA has conducted an extensive redesign effort for the Space Shutle in the aftermath of the STS 51-L Challenger accident, encompassing not only Shuttle vehicle and booster design but also such system-wide factors as organizational structure, management procedures, flight safety, flight operations, sustainable flight rate, and maintenance safeguards. Attention is presently given to Solid Rocket Booster redesign features, the Shuttle Main Engine's redesigned high pressure fuel and oxidizer turbopumps, the Shuttle Orbiter's braking and rollout (landing gear) system, the entry control mode of the flight control system, a 'split-S' abort maneuver for the Orbiter, and crew escape capsule proposals.

Space and man. [planetary exploration and energy sources

NASA Technical Reports Server (NTRS)

Kolman, E.

1974-01-01

The effects of man's entry into space on changes in economics and technology, politics and law, science, philosophy, and art are considered. A single world economy, extracting from the natural resources of the moon and other cosmic bodies raw materials and energy, will avoid terrestrial limitations and improve society by eliminating the inequalities of economic and social status. However, a spacecraft for interplanetary travel require thermonuclear engines that achieve an escape velocity of 0.1 times the speed of light in order to allow an astronaut stellar expedition corresponding to the active life of a single generation.

Robotic Stripping

NASA Technical Reports Server (NTRS)

2000-01-01

UltraStrip Systems, Inc.'s M-200 removes paint from the hulls of ships faster than traditional grit-blasting methods. And, it does so without producing toxic airborne particles common to traditional methods. The M-2000 magnetically attaches itself to the hull of the ship. Its water jets generate 40,000 pounds of pressure per square inch, blasting away paint down to the ships steel substrate. The only by product is water and dried paint chips and these are captured by a vacuum system so no toxic residue can escape. It was built out of a partnership between the Jet Propulsion Laboratory and the National Robotics Engineering Consortium.

Managing Pacific salmon escapements: The gaps between theory and reality

USGS Publications Warehouse

Knudsen, E. Eric; Knudsen, E. Eric; Steward, Cleveland R.; MacDonald, Donald D.; Williams, Jack E.; Reiser, Dudley W.

1999-01-01

There are myriad challenges to estimating intrinsic production capacity for Pacific salmon populations that are heavily exploited and/or suffering from habitat alteration. Likewise, it is difficult to determine whether perceived decreases in production are due to harvest, habitat, or hatchery influences, natural variation, or some combination of all four. There are dramatic gaps between the true nature of the salmon spawner/recruit relationship and the theoretical basis for describing and understanding the relationship. Importantly, there are also extensive practical difficulties associated with gathering and interpreting accurate escapement and run-size information and applying it to population management. Paradoxically, certain aspects of salmon management may well be contributing to losses in abundance and biodiversity, including harvesting salmon in mixed population fisheries, grouping populations into management units subject to a common harvest rate, and fully exploiting all available hatchery fish at the expense of wild fish escapements. Information on U.S. Pacific salmon escapement goal-setting methods, escapement data collection methods and estimation types, and the degree to which stocks are subjected to mixed stock fisheries was summarized and categorized for 1,025 known management units consisting of 9,430 known populations. Using criteria developed in this study, only 1% of U.S. escapement goals are by methods rated as excellent. Escapement goals for 16% of management units were rated as good. Over 60% of escapement goals have been set by methods rated as either fair or poor and 22% of management units have no escapement goals at all. Of the 9,430 populations for which any information was available, 6,614 (70%) had sufficient information to categorize the method by which escapement data are collected. Of those, data collection methods were rated as excellent for 1%, good for 1%, fair for 2%, and poor for 52%. Escapement estimates are not made for 44% of populations. Escapement estimation type (quality of the data resulting from survey methods) was rated as excellent for <1%, good for 30%, fair for 3%, poor for 22%, and nonexistent for 45%. Numerous recommendations for improvements in escapement mangement are made in this chapter. In general, improvements are needed on theoretical escapement management techniques, escapement goal setting methods, and escapement and run size data quality. There is also a need to change managers' and harvesters' expectations to coincide with the natural variation and uncertainty in the abundance of salmon populations. All the recommendations are aimed at optimizing the number of spawners-healthy escapements ensure salmon sustainability by providing eggs for future production, nutrients to the system, and genetic diversity.

Activity map of the tammar X chromosome shows that marsupial X inactivation is incomplete and escape is stochastic

PubMed Central

2010-01-01

Background X chromosome inactivation is a spectacular example of epigenetic silencing. In order to deduce how this complex system evolved, we examined X inactivation in a model marsupial, the tammar wallaby (Macropus eugenii). In marsupials, X inactivation is known to be paternal, incomplete and tissue-specific, and occurs in the absence of an XIST orthologue. Results We examined expression of X-borne genes using quantitative PCR, revealing a range of dosage compensation for different loci. To assess the frequency of 1X- or 2X-active fibroblasts, we investigated expression of 32 X-borne genes at the cellular level using RNA-FISH. In female fibroblasts, two-color RNA-FISH showed that genes were coordinately expressed from the same X (active X) in nuclei in which both loci were inactivated. However, loci on the other X escape inactivation independently, with each locus showing a characteristic frequency of 1X-active and 2X-active nuclei, equivalent to stochastic escape. We constructed an activity map of the tammar wallaby inactive X chromosome, which identified no relationship between gene location and extent of inactivation, nor any correlation with the presence or absence of a Y-borne paralog. Conclusions In the tammar wallaby, one X (presumed to be maternal) is expressed in all cells, but genes on the other (paternal) X escape inactivation independently and at characteristic frequencies. The paternal and incomplete X chromosome inactivation in marsupials, with stochastic escape, appears to be quite distinct from the X chromosome inactivation process in eutherians. We find no evidence for a polar spread of inactivation from an X inactivation center. PMID:21182760

The Real Cost: Know the Real Cost of Tobacco

MedlinePlus

... LINK IS COPIED TO CLIPBOARD Brain Escape The Game Billboard screen loads the game and displays the text “Brain Escape Addiction from smoking is hard to escape.” This game is called Brain Escape. The objective is to ...

Recent advances in synthetic biosafety

PubMed Central

Simon, Anna J.; Ellington, Andrew D.

2016-01-01

Synthetically engineered organisms hold promise for a broad range of medical, environmental, and industrial applications. Organisms can potentially be designed, for example, for the inexpensive and environmentally benign synthesis of pharmaceuticals and industrial chemicals, for the cleanup of environmental pollutants, and potentially even for biomedical applications such as the targeting of specific diseases or tissues. However, the use of synthetically engineered organisms comes with several reasonable safety concerns, one of which is that the organisms or their genes could escape their intended habitats and cause environmental disruption. Here we review key recent developments in this emerging field of synthetic biocontainment and discuss further developments that might be necessary for the widespread use of synthetic organisms. Specifically, we discuss the history and modern development of three strategies for the containment of synthetic microbes: addiction to an exogenously supplied ligand; self-killing outside of a designated environment; and self-destroying encoded DNA circuitry outside of a designated environment. PMID:27635235

An algorithm for engineering regime shifts in one-dimensional dynamical systems

NASA Astrophysics Data System (ADS)

Tan, James P. L.

2018-01-01

Regime shifts are discontinuous transitions between stable attractors hosting a system. They can occur as a result of a loss of stability in an attractor as a bifurcation is approached. In this work, we consider one-dimensional dynamical systems where attractors are stable equilibrium points. Relying on critical slowing down signals related to the stability of an equilibrium point, we present an algorithm for engineering regime shifts such that a system may escape an undesirable attractor into a desirable one. We test the algorithm on synthetic data from a one-dimensional dynamical system with a multitude of stable equilibrium points and also on a model of the population dynamics of spruce budworms in a forest. The algorithm and other ideas discussed here contribute to an important part of the literature on exercising greater control over the sometimes unpredictable nature of nonlinear systems.

Nano Polymeric Carrier Fabrication Technologies for Advanced Antitumor Therapy

PubMed Central

Li, Wei; Zhao, Mengxin; Ke, Changhong; Zhang, Ge; Zhang, Li; Li, Huafei; Zhang, Fulei; Sun, Yun; Dai, Jianxin; Wang, Hao; Guo, Yajun

2013-01-01

Comparing with the traditional therapeutic methods, newly developed cancer therapy based on the nanoparticulates attracted extensively interest due to its unique advantages. However, there are still some drawbacks such as the unfavorable in vivo performance for nanomedicine and undesirable tumor escape from the immunotherapy. While as we know that the in vivo performance strongly depended on the nanocarrier structural properties, thus, the big gap between in vitro and in vivo can be overcome by nanocarrier's structural tailoring by fine chemical design and microstructural tuning. In addition, this fine nanocarrier's engineering can also provide practical solution to solve the problems in traditional cancer immunotherapy. In this paper, we review the latest development in nanomedicine, cancer therapy, and nanoimmunotherapy. We then give an explanation why fine nanocanrrie's engineering with special focus on the unique pathology of tumor microenvironments and properties of immunocells can obviously promote the in vivo performance and improve the therapeutic index of nanoimmunotherapy. PMID:24369011

Next Generation Launch Technology Program Lessons Learned

NASA Technical Reports Server (NTRS)

Cook, Stephen; Tyson, Richard

2005-01-01

In November 2002, NASA revised its Integrated Space Transportation Plan (ISTP) to evolve the Space Launch Initiative (SLI) to serve as a theme for two emerging programs. The first of these, the Orbital Space Plane (OSP), was intended to provide crew-escape and crew-transfer functions for the ISS. The second, the NGLT Program, developed technologies needed for safe, routine space access for scientific exploration, commerce, and national defense. The NGLT Program was comprised of 12 projects, ranging from fundamental high-temperature materials research to full-scale engine system developments (turbine and rocket) to scramjet flight test. The Program included technology advancement activities with a broad range of objectives, ultimate applications/timeframes, and technology maturity levels. An over-arching Systems Engineering and Analysis (SE&A) approach was employed to focus technology advancements according to a common set of requirements. Investments were categorized into three segments of technology maturation: propulsion technologies, launch systems technologies, and SE&A.

Nano polymeric carrier fabrication technologies for advanced antitumor therapy.

PubMed

Li, Wei; Zhao, Mengxin; Ke, Changhong; Zhang, Ge; Zhang, Li; Li, Huafei; Zhang, Fulei; Sun, Yun; Dai, Jianxin; Wang, Hao; Guo, Yajun

2013-01-01

Comparing with the traditional therapeutic methods, newly developed cancer therapy based on the nanoparticulates attracted extensively interest due to its unique advantages. However, there are still some drawbacks such as the unfavorable in vivo performance for nanomedicine and undesirable tumor escape from the immunotherapy. While as we know that the in vivo performance strongly depended on the nanocarrier structural properties, thus, the big gap between in vitro and in vivo can be overcome by nanocarrier's structural tailoring by fine chemical design and microstructural tuning. In addition, this fine nanocarrier's engineering can also provide practical solution to solve the problems in traditional cancer immunotherapy. In this paper, we review the latest development in nanomedicine, cancer therapy, and nanoimmunotherapy. We then give an explanation why fine nanocanrrie's engineering with special focus on the unique pathology of tumor microenvironments and properties of immunocells can obviously promote the in vivo performance and improve the therapeutic index of nanoimmunotherapy.

Demonstration of optical computing logics based on binary decision diagram.

PubMed

Lin, Shiyun; Ishikawa, Yasuhiko; Wada, Kazumi

2012-01-16

Optical circuits are low power consumption and fast speed alternatives for the current information processing based on transistor circuits. However, because of no transistor function available in optics, the architecture for optical computing should be chosen that optics prefers. One of which is Binary Decision Diagram (BDD), where signal is processed by sending an optical signal from the root through a serial of switching nodes to the leaf (terminal). Speed of optical computing is limited by either transmission time of optical signals from the root to the leaf or switching time of a node. We have designed and experimentally demonstrated 1-bit and 2-bit adders based on the BDD architecture. The switching nodes are silicon ring resonators with a modulation depth of 10 dB and the states are changed by the plasma dispersion effect. The quality, Q of the rings designed is 1500, which allows fast transmission of signal, e.g., 1.3 ps calculated by a photon escaping time. A total processing time is thus analyzed to be ~9 ps for a 2-bit adder and would scales linearly with the number of bit. It is two orders of magnitude faster than the conventional CMOS circuitry, ~ns scale of delay. The presented results show the potential of fast speed optical computing circuits.

Using Due Process Opinions as an Opportunity to Improve Educational Practice

ERIC Educational Resources Information Center

Rock, Marcia L.; Bateman, David

2009-01-01

There is little question that today's society is a litigious one, and few professions can escape the realities of such. Although there is no mandate for national data collection on special education due process hearings (Ahearn, 2002), Chambers, Harr, and Dhanani (2003) estimate that more than 9,000 requests for dispute resolution are made per…

Composition of the Solar Wind

NASA Technical Reports Server (NTRS)

Suess, S. T.

2007-01-01

The solar wind reflects the composition of the Sun and physical processes in the corona. Analysis produces information on how the solar system was formed and on physical processes in the corona. The analysis can also produce information on the local interstellar medium, galactic evolution, comets in the solar wind, dust in the heliosphere, and matter escaping from planets.

Solar Thermal Propulsion Improvements at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

Gerrish, Harold P.

2003-01-01

Solar Thermal Propulsion (STP) is a concept which operates by transferring solar energy to a propellant, which thermally expands through a nozzle. The specific impulse performance is about twice that of chemical combustions engines, since there is no need for an oxidizer. In orbit, an inflatable concentrator mirror captures sunlight and focuses it inside an engine absorber cavity/heat exchanger, which then heats the propellant. The primary application of STP is with upperstages taking payloads from low earth orbit to geosynchronous earth orbit or earth escape velocities. STP engines are made of high temperature materials since heat exchanger operation requires temperatures greater than 2500K. Refractory metals such as tungsten and rhenium have been examined. The materials must also be compatible with hot hydrogen propellant. MSFC has three different engine designs, made of different refractory metal materials ready to test. Future engines will be made of high temperature carbide materials, which can withstand temperatures greater than 3000K, hot hydrogen, and provide higher performance. A specific impulse greater than 1000 seconds greatly reduces the amount of required propellant. A special 1 OkW solar ground test facility was made at MSFC to test various STP engine designs. The heliostat mirror, with dual-axis gear drive, tracks and reflects sunlight to the 18 ft. diameter concentrator mirror. The concentrator then focuses sunlight through a vacuum chamber window to a small focal point inside the STP engine. The facility closely simulates how the STP engine would function in orbit. The flux intensity at the focal point is equivalent to the intensity at a distance of 7 solar radii from the sun.

Effects of different drivers on ion fluxes at Mars. MARS EXPRESS and MAVEN observations

NASA Astrophysics Data System (ADS)

Dubinin, Eduard; Fraenz, Markus; McFadden, James; Halekas, Jasper; Epavier, Frank; Connerney, Jack; Brain, David; Jakosky, Bruce; Andrews, David; Barabash, Stas

2017-04-01

Recent observations by Mars Express and MAVEN spacecraft have shown that the Martian atmosphere/ionosphere is exposed to the impact of solar wind which results in losses of volatiles from Mars. This erosion is an important factor for the evolution of the Martian atmosphere and its water inventory. To estimate the escape forced by the solar wind during the early Solar System conditions we need to know how the ionosphere of Mars and escape fluxes depend on variations in the strength of the external drivers, in particularly, of solar wind and solar EUV flux. We present multi-instrument observations of the influence of the solar wind and solar irradiance on the Martian ionosphere and escape fluxes. We use data obtained by the ASPERA-3 and MARSIS experiments on Mars Express and by the STATIC, SWIA, MAG and EUV monitor on MAVEN. Observations by Mars Express supplemented by the EUV monitoring at Earth orbit and translated to Mars orbit provide us information about this dependence over more than 10 years whereas the measurements made by MAVEN provide us for the first time the opportunity to study these processes with simultaneous monitoring of the solar wind and ionospheric variations, planetary ion fluxes and solar irradiance. It will be shown that that fluxes of planetary ions through different escape channels (trans-terminator fluxes, ion plume, plasma sheet) respond differently on the variations of the different drivers.

InhA1-Mediated Cleavage of the Metalloprotease NprA Allows Bacillus cereus to Escape From Macrophages.

PubMed

Haydar, Abbass; Tran, Seav-Ly; Guillemet, Elisabeth; Darrigo, Claire; Perchat, Stéphane; Lereclus, Didier; Coquet, Laurent; Jouenne, Thierry; Ramarao, Nalini

2018-01-01

Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation in the host despite the induction of inflammation. To circumvent inflammation, bacteria must resist the bactericidal activity of professional phagocytes, which constitute a first line of host defense against pathogens. Interactions between phagocytic cells and B. cereus are still poorly characterized and the mechanism of resistance to the host immune system is not known yet. We have previously shown that the spores are phagocytosed by macrophages but survive and escape from these cells. The metalloprotease InhA1 is a key effector involved in these processes. inhA1 -deficient spores are retained intracellularly, in contrast to the wild type strain spores. NprA is also a B. cereus metalloprotease able to cleave tissue components such as fibronectin, laminin, and collagen. Here, we show that NprA, concomitantly secreted with InhA1 in the B. cereus secretome, is essential to promote bacterial escape from macrophages. We show that InhA1 cleaves NprA at specific sites. This cleavage allows liberation of the mature form of the NprA protein in the supernatant of the wild type strain. This mature form of NprA is actually the principal effector allowing bacterial escape from host macrophages.

Bioactive glass 13-93 as a subchondral substrate for tissue-engineered osteochondral constructs: a pilot study.

PubMed

Jayabalan, Prakash; Tan, Andrea R; Rahaman, Mohammed N; Bal, B Sonny; Hung, Clark T; Cook, James L

2011-10-01

Replacement of diseased areas of the joint with tissue-engineered osteochondral grafts has shown potential in the treatment of osteoarthritis. Bioactive glasses are candidates for the osseous analog of these grafts. (1) Does Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate improve collagen and glycosaminoglycan production in a tissue-engineered cartilage layer? (2) Does BG 13-93 as a culture medium supplement increase the collagen and glycosaminoglycan production and improve the mechanical properties in a tissue-engineered cartilage layer? In Study 1, bioactive glass samples (n = 4) were attached to a chondrocyte-seeded agarose layer to form an osteochondral construct, cultured for 6 weeks, and compared to controls. In Study 2, bioactive glass samples (n = 5) were cocultured with cell-seeded agarose for 6 weeks. The cell-seeded agarose layer was exposed to BG 13-93 either continuously or for the first or last 2 weeks in culture or had no exposure. Osteochondral constructs with a BG 13-93 base had improved glycosaminoglycan deposition but less collagen II content. Agarose scaffolds that had a temporal exposure to BG 13-93 within the culture medium had improved mechanical and biochemical properties compared to continuous or no exposure. When used as a subchondral substrate, BG 13-93 did not improve biochemical properties compared to controls. However, as a culture medium supplement, BG 13-93 improved the biochemical and mechanical properties of a tissue-engineered cartilage layer. BG 13-93 may not be suitable in osteochondral constructs but could have potential as a medium supplement for neocartilage formation.

Interface engineering of Cu(In,Ga)Se2 and atomic layer deposited Zn(O,S) heterojunctions

NASA Astrophysics Data System (ADS)

Schmidt, Sebastian S.; Merdes, Saoussen; Steigert, Alexander; Klenk, Reiner; Kaufmann, Christian A.; Simsek Sanli, Ekin; van Aken, Peter A.; Oertel, Mike; Schneikart, Anja; Dimmler, Bernhard; Schlatmann, Rutger

2017-08-01

Atomic layer deposition of Zn(O,S) is an attractive dry and Cd-free process for the preparation of buffer layers for chalcopyrite solar modules. As we previously reported, excellent cell and module efficiencies were achieved using absorbers from industrial pilot production. These absorbers were grown using a selenization/sulfurization process. In this contribution we report on the interface engineering required to adapt the process to sulfur-free multi source evaporated absorbers. Different approaches to a local sulfur enrichment at the heterojunction have been studied by using surface analysis (XPS) and scanning transmission electron microscopy. We correlate the microstructure and element distribution at the interface with device properties obtained by electronic characterization. The optimized completely dry process yields cell efficiencies >16% and 30 × 30 cm2 minimodule efficiencies of up to 13.9% on industrial substrates. Any degradation observed in the dry heat stress test is fully reversible after light soaking.

16. INTERIOR VIEW OF SUBMARINE SECTION AT 110FOOT LEVEL, ESCAPE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. INTERIOR VIEW OF SUBMARINE SECTION AT 110-FOOT LEVEL, ESCAPE TRAINING TANK, SHOWING LADDER TO ESCAPE TANK, LOOKING SOUTH - U.S. Naval Submarine Base, New London Submarine Escape Training Tank, Albacore & Darter Roads, Groton, New London County, CT

Design and Testing of an H2/O2 Predetonator for a Simulated Rotating Detonation Engine Channel

DTIC Science & Technology

2013-03-01

Diameter PDE Pulse Detonation Engines RDE Rotating Detonation Engine WPAFB Wright Patterson Air Force Base ZND Zeldovich, von Neumann and Doring xv...DESIGN AND TESTING OF AN H2/O2 PREDETONATOR FOR A SIMULATED ROTATING DETONATION ENGINE CHANNEL THESIS Stephen J. Miller, 2Lt, USAF AFIT-ENY-13-M-23...RELEASE; DISTRIBUTION UNLIMITED AFIT-ENY-13-M-23 DESIGN AND TESTING OF AN H2/O2 PREDETONATOR FOR A SIMULATED ROTATING DETONATION ENGINE CHANNEL Stephen

Solar forcing, and ionospheric ion outflow from Venus, Earth and Mars - A comparison

NASA Astrophysics Data System (ADS)

Lundin, R. N.

2012-12-01

Solar forcing by e.g. EUV radiation and the solar wind leads to outflow and escape of ionospheric ions from Earth, Venus and Mars. In-situ measurements in the Earth's space environment have demonstrated that the ion escape rate correlates with the magnitude of solar forcing, i.e. high solar EUV and solar wind forcing leads to enhanced escape rates. The Terrestrial outflow is dominated by H+ and O+ suggesting that the ultimate origin of outflowing ions is water. Recent measurements from the two arid planets Mars and Venus, their atmospheres dominated by CO2, display characteristics similar to that of the Earth - an outflow dominated by hydrogen (H+) and oxygen (O+, O2+) ions. Despite major differences in atmospheric composition, the composition of the ion outflow from Earth and Venus is very similar, i.e. H+ and O+ dominates and the outflow has a stoichiometric H/O ratio of close to 2. The latter implies escape of water. The ion outflow from Mars is dominated by O+, O2+, and H+. Here the stoichiometric ratio between hydrogen and oxygen ion is ≈1, implying that if the ion outflow originates from water, about half of the hydrogen mass disappears by other means. The primary origin of the ion outflow from Earth, Venus and Mars is a complex issue. Nevertheless, a predominant hydrogen and oxygen loss implies that water can easily escape planets orbiting close to the Sun, while Carbon-based molecules (e.g. CO2) resides more easily. Observations shows that the outflow of e.g. CO+ and CO2+ from Mars and Venus is minute compared to the outflow of hydrogen and oxygen ions. Magnetic shielding is an issue affecting the net ion outflow and escape from a planet, because acceleration processes are also the characteristics of magnetized plasmas. Recent findings suggests that, despite magnetic field pile-up at Mars and Venus, the stand-off distance is insufficient to prohibit a direct interaction between the solar wind and the magnetized ionospheric plasma in the induced magnetospheres of Mars and Venus. On the other hand, a planetary magnetic field, such as the Earth's dipole field and the Martian multipole crustal field, may foster shielding as well as plasma acceleration. However, in this case the ion acceleration may be confined in closed planetary magnetic flux tubes, leading to a low escape rates.

Effect of Tooling Material on the Internal Surface Quality of Ti6Al4V Parts Fabricated by Hot Isostatic Pressing

NASA Astrophysics Data System (ADS)

Cai, Chao; Song, Bo; Wei, Qingsong; Yan, Wu; Xue, Pengju; Shi, Yusheng

2017-01-01

For the net-shape hot isostatic pressing (HIP) process, control of the internal surface roughness of as-HIPped parts remains a challenge for practical engineering. To reveal the evolution mechanism of the internal surface of the parts during the HIP process, the effect of different tooling materials (H13, T8, Cr12 steel, and graphite) as internal cores on the interfacial diffusion and surface roughness was systematically studied.

Quantifying factors determining the rate of CTL escape and reversion during acute and chronic phases of HIV infection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ganusov, Vitaly V; Korber, Bette M; Perelson, Alan S

Human immunodeficiency virus (HIV) often evades cytotoxic T cell (CTL) responses by generating variants that are not recognized by CTLs. However, the importance and quantitative details of CTL escape in humans are poorly understood. In part, this is because most studies looking at escape of HIV from CTL responses are cross-sectional and are limited to early or chronic phases of the infection. We use a novel technique of single genome amplification (SGA) to identify longitudinal changes in the transmitted/founder virus from the establishment of infection to the viral set point at 1 year after the infection. We find that HIVmore » escapes from virus-specific CTL responses as early as 30-50 days since the infection, and the rates of viral escapes during acute phase of the infection are much higher than was estimated in previous studies. However, even though with time virus acquires additional escape mutations, these late mutations accumulate at a slower rate. A poor correlation between the rate of CTL escape in a particular epitope and the magnitude of the epitope-specific CTL response suggests that the lower rate of late escapes is unlikely due to a low efficacy of the HIV-specific CTL responses in the chronic phase of the infection. Instead, our results suggest that late and slow escapes are likely to arise because of high fitness cost to the viral replication associated with such CTL escapes. Targeting epitopes in which virus escapes slowly or does not escape at all by CTL responses may, therefore, be a promising direction for the development of T cell based HIV vaccines.« less

Genes That Escape X-Inactivation in Humans Have High Intraspecific Variability in Expression, Are Associated with Mental Impairment but Are Not Slow Evolving

PubMed Central

Zhang, Yuchao; Castillo-Morales, Atahualpa; Jiang, Min; Zhu, Yufei; Hu, Landian; Urrutia, Araxi O.; Kong, Xiangyin; Hurst, Laurence D.

2013-01-01

In female mammals most X-linked genes are subject to X-inactivation. However, in humans some X-linked genes escape silencing, these escapees being candidates for the phenotypic aberrations seen in polyX karyotypes. These escape genes have been reported to be under stronger purifying selection than other X-linked genes. Although it is known that escape from X-inactivation is much more common in humans than in mice, systematic assays of escape in humans have to date employed only interspecies somatic cell hybrids. Here we provide the first systematic next-generation sequencing analysis of escape in a human cell line. We analyzed RNA and genotype sequencing data obtained from B lymphocyte cell lines derived from Europeans (CEU) and Yorubans (YRI). By replicated detection of heterozygosis in the transcriptome, we identified 114 escaping genes, including 76 not previously known to be escapees. The newly described escape genes cluster on the X chromosome in the same chromosomal regions as the previously known escapees. There is an excess of escaping genes associated with mental retardation, consistent with this being a common phenotype of polyX phenotypes. We find both differences between populations and between individuals in the propensity to escape. Indeed, we provide the first evidence for there being both hyper- and hypo-escapee females in the human population, consistent with the highly variable phenotypic presentation of polyX karyotypes. Considering also prior data, we reclassify genes as being always, never, and sometimes escape genes. We fail to replicate the prior claim that genes that escape X-inactivation are under stronger purifying selection than others. PMID:24023392

Effect of thiol pendant conjugates on plasmid DNA binding, release, and stability of polymeric delivery vectors.

PubMed

Bacalocostantis, Irene; Mane, Viraj P; Kang, Michael S; Goodley, Addison S; Muro, Silvia; Kofinas, Peter

2012-05-14

Polymers have attracted much attention as potential gene delivery vectors due to their chemical and structural versatility. However, several challenges associated with polymeric carriers, including low transfection efficiencies, insufficient cargo release, and high cytotoxicity levels have prevented clinical implementation. Strong electrostatic interactions between polymeric carriers and DNA cargo can prohibit complete cargo release within the cell. As a result, cargo DNA never reaches the cell's nucleus where gene expression takes place. In addition, highly charged cationic polymers have been correlated with high cytotoxicity levels, making them unsuitable carriers in vivo. Using poly(allylamine) (PAA) as a model, we investigated how pH-sensitive disulfide cross-linked polymer networks can improve the delivery potential of cationic polymer carriers. To accomplish this, we conjugated thiol-terminated pendant chains onto the primary amines of PAA using 2-iminothiolane, developing three new polymer vectors with 5, 13, or 20% thiol modification. Unmodified PAA and thiol-conjugated polymers were tested for their ability to bind and release plasmid DNA, their capacity to protect genetic cargo from enzymatic degradation, and their potential for endolysosomal escape. Our results demonstrate that polymer-plasmid complexes (polyplexes) formed by the 13% thiolated polymer demonstrate the greatest delivery potential. At high N/P ratios, all thiolated polymers (but not unmodified counterparts) were able to resist decomplexation in the presence of heparin, a negatively charged polysaccharide used to mimic in vivo polyplex-protein interactions. Further, all thiolated polymers exhibited higher buffering capacities than unmodified PAA and, therefore, have a greater potential for endolysosomal escape. However, 5 and 20% thiolated polymers exhibited poor DNA binding-release kinetics, making them unsuitable carriers for gene delivery. The 13% thiolated polymers, on the other hand, displayed high DNA binding efficiency and pH-sensitive release.

The effect of widespread early aerobic marine ecosystems on methane cycling and the Great Oxidation

NASA Astrophysics Data System (ADS)

Daines, Stuart J.; Lenton, Timothy M.

2016-01-01

The balance of evidence suggests that oxygenic photosynthesis had evolved by 3.0-2.7 Ga, several hundred million years prior to the Great Oxidation ≈2.4 Ga. Previous work has shown that if oxygenic photosynthesis spread globally prior to the Great Oxidation, this could have supported widespread aerobic ecosystems in the surface ocean, without oxidising the atmosphere. Here we use a suite of models to explore the implications for carbon cycling and the Great Oxidation. We find that recycling of oxygen and carbon within early aerobic marine ecosystems would have restricted the balanced fluxes of methane and oxygen escaping from the ocean, lowering the atmospheric concentration of methane in the Great Oxidation transition and its aftermath. This in turn would have minimised any bi-stability of atmospheric oxygen, by weakening a stabilising feedback on oxygen from hydrogen escape to space. The result would have been a more reversible and probably episodic rise of oxygen at the Great Oxidation transition, consistent with existing geochemical evidence. The resulting drop in methane levels to ≈10 ppm is consistent with climate cooling at the time but adds to the puzzle of what kept the rest of the Proterozoic warm. A key test of the scenario of abundant methanotrophy in oxygen oases before the Great Oxidation is its predicted effects on the organic carbon isotope (δ13Corg) record. Our open ocean general circulation model predicts δC13org ≈ - 30 to -45‰ consistent with most data from 2.65 to 2.45 Ga. However, values of δC13org ≈ - 50 ‰ require an extreme scenario such as concentrated methanotroph production where shelf-slope upwelling of methane-rich water met oxic shelf water.

Attitude Determination and Control Systems

NASA Technical Reports Server (NTRS)

Starin, Scott R.; Eterno, John

2010-01-01

The importance of accurately pointing spacecraft to our daily lives is pervasive, yet somehow escapes the notice of most people. In this section, we will summarize the processes and technologies used in designing and operating spacecraft pointing (i.e. attitude) systems.

Mars, Venus, Earth and Titan UV Laboratory Aeronomy by Electron Impact

NASA Astrophysics Data System (ADS)

Malone, C. P.; Ajello, J. M.; McClintock, W. E.; Eastes, R.; Evans, J. S.; Holsclaw, G.; Schneider, N. M.; Jain, S.; Gerard, J. C. M. C.; Hoskins, A.

2017-12-01

The UV response of the Mars, Earth, Titan and Venus upper atmospheres to the solar radiation fields [solar wind and solar EUV] is the focus of the present generation of Mars, Earth, Titan and Venus missions. These missions are Mars Express (MEX), the Mars Atmosphere and Volatile Evolution Mission (MAVEN), Cassini at Titan, Global-scale Observations of the Limb and Disk (GOLD) mission for Earth and Venus Express (VEX). Each spacecraft is equipped with a UV spectrometer that senses far ultraviolet (FUV) emissions from 110-190 nm, whose dayglow intensities are proportional to three quantities:1) particle (electron, ion) fluxes, 2) the altitude distribution of species in the ionosphere: CO, CO2, O, N2 at Venus and Mars and N2, O and O2 at Titan and Earth and 3) the emission cross section for the interaction process. UV spectroscopy provides a benchmark to the present space environment and indicates pathways for removing upper atmosphere gas (e.g., water escape from Mars and Earth) or N2 escape at Titan over eons. We present a UV laboratory program that utilizes an instrument, unique in the world, at the University of Colorado that can measure excitation mechanisms by particle (electron, ion) impact and the resulting emission cross sections that include processes occurring in a planetary atmosphere, particularly the optically forbidden emissions presented by the Cameron bands, the Lyman Birge Hopfield bands and the OI 135.6 nm multiplet. There are presently uncertainties by a factor of two in the existing measurements of the emission cross section, affecting modeling of electron transport. We have utilized the MAVEN Imaging Ultraviolet Spectrograph (IUVS) engineering model which operates at moderate spectral resolution ( 0.5-1.0nm FWHM) to obtain the full vibrational spectra of the Cameron band system CO(a 3Π → X 1Σ+) from both CO direct excitation and CO2 dissociative excitation, and for the dipole-allowed Fourth Positive band system of CO, while for N2 we have studied molecular nitrogen (N2 LBH bands, a 1Πg → X 1Σg+). We have performed laboratory measurements using mono-energetic electrons in a large chamber to excite band systems by the same processes as occur at low densities in planetary atmospheres. We have ascertained vibrational structure and emission cross sections for the strongest band systems on solar system objects.

Diffusive dynamics of nanoparticles in ultra-confined media

DOE PAGES

Jacob, Jack Deodato; Conrad, Jacinta; Krishnamoorti, Ramanan; ...

2015-08-10

Differential dynamic microscopy (DDM) was used to investigate the diffusive dynamics of nanoparticles of diameter 200 400 nm that were strongly confined in a periodic square array of cylindrical nanoposts. The minimum distance between posts was 1.3 5 times the diameter of the nanoparticles. The image structure functions obtained from the DDM analysis were isotropic and could be fit by a stretched exponential function. The relaxation time scaled diffusively across the range of wave vectors studied, and the corresponding scalar diffusivities decreased monotonically with increased confinement. The decrease in diffusivity could be described by models for hindered diffusion that accountedmore » for steric restrictions and hydrodynamic interactions. The stretching exponent decreased linearly as the nanoparticles were increasingly confined by the posts. Altogether, these results are consistent with a picture in which strongly confined nanoparticles experience a heterogeneous spatial environment arising from hydrodynamics and volume exclusion on time scales comparable to cage escape, leading to multiple relaxation processes and Fickian but non-Gaussian diffusive dynamics.« less

Impact Craters on Titan? Cassini RADAR View

NASA Technical Reports Server (NTRS)

Wood, Charles A.; Lopes, Rosaly; Stofan, Ellen R.; Paganelli, Flora; Elachi, Charles

2005-01-01

Titan is a planet-size (diameter of 5,150 km) satellite of Saturn that is currently being investigated by the Cassini spacecraft. Thus far only one flyby (Oct. 26, 2004; Ta) has occurred when radar images were obtained. In February, 2005, and approximately 20 more times in the next four years, additional radar swaths will be acquired. Each full swath images about 1% of Titan s surface at 13.78 GHz (Ku-band) with a maximum resolution of 400 m. The Ta radar pass [1] demonstrated that Titan has a solid surface with multiple types of landforms. However, there is no compelling detection of impact craters in this first radar swath. Dione, Tethys and other satellites of Saturn are intensely cratered, there is no way that Titan could have escaped a similar impact cratering past; thus there must be ongoing dynamic surface processes that erase impact craters (and other landforms) on Titan. The surface of Titan must be very young and the resurfacing rate must be significantly higher than the impact cratering rate.

Floating marine debris surface drift: convergence and accumulation toward the South Pacific subtropical gyre.

PubMed

Martinez, Elodie; Maamaatuaiahutapu, Keitapu; Taillandier, Vincent

2009-09-01

Whatever its origin is, a floating particle at the sea surface is advected by ocean currents. Surface currents could be derived from in situ observations or combined with satellite data. For a better resolution in time and space, we use satellite-derived sea-surface height and wind stress fields with a 1/3 degrees grid from 1993 to 2001 to determine the surface circulation of the South Pacific Ocean. Surface currents are then used to compute the Lagrangian trajectories of floating debris. Results show an accumulation of the debris in the eastern-centre region of the South Pacific subtropical gyre ([120 degrees W; 80 degrees W]-[20 degrees S; 40 degrees S]), resulting from a three-step process: in the first two years, mostly forced by Ekman drift, the debris drift towards the tropical convergence zone ( approximately 30 degrees S). Then they are advected eastward mostly forced by geostrophic currents. They finally reach the eastern-centre region of the South Pacific subtropical gyre from where they could not escape.

30 CFR 75.382 - Mechanical escape facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Mechanical escape facilities. 75.382 Section 75... HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.382 Mechanical escape facilities. (a) Mechanical escape facilities shall be provided with overspeed, overwind, and automatic stop...

Physics escape room as an educational tool

NASA Astrophysics Data System (ADS)

Vörös, Alpár István Vita; Sárközi, Zsuzsa

2017-12-01

Escape rooms have flourished in the last decade. These are adventure games in which players work together to solve puzzles using hints, clues and a strategy to escape from a locked room. In many cases they use different phenomena related to physics. Hence the idea of using escape rooms in science centers or even in classroom activities. Escape rooms are designed for one single team of players, the method is more suitable for activities in a science centre. In our paper, we show that escape rooms' puzzle solving methods could be used in physics classroom activities as well, taking into account that several teams have to work together in the same room/place. We have developed an educational escape game for physics of fluids, as this topic is left out from the Romanian high-school curriculum. We have tried out our game during the project week called "Şcoala altfel" ("school in a different way") and in a physics camp for gifted students. We present the designed physics escape game and the results.

Prevalence of residential smoke alarms and fire escape plans in the U.S.: results from the Second Injury Control and Risk Survey (ICARIS-2).

PubMed

Ballesteros, Michael F; Kresnow, Marcie-Jo

2007-01-01

This study was conducted to estimate (1) the proportion of U.S. homes with installed smoke alarms and fire escape plans, and (2) the frequency of testing home smoke alarms and of practicing the fire escape plans. The authors analyzed data on smoke alarms and fire escape plans from a national cross-sectional random-digit dialed telephone survey of 9,684 households. Ninety-five percent of surveyed households reported at least one installed smoke alarm and 52% had a fire escape plan. The prevalence of alarms varied by educational level, income, and the presence of a child in the home. Only 15% tested their alarms once a month and only 16% of homes with an escape plan reported practicing it every six months. While smoke alarm prevalence in U.S. homes is high, only half of homes have a fire escape plan. Additional emphasis is needed on testing of installed smoke alarms and on preparedness for fire escape plans.

46 CFR 13.501 - Original application for “Tankerman-Engineer” endorsement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Original application for âTankerman-Engineerâ endorsement. 13.501 Section 13.501 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE... Original application for “Tankerman-Engineer” endorsement. Each applicant for a “Tankerman-Engineer...

Highway Safety Program Manual: Volume 13: Traffic Engineering Services.

ERIC Educational Resources Information Center

National Highway Traffic Safety Administration (DOT), Washington, DC.

Volume 13 of the 19-volume Highway Safety Program Manual (which provides guidance to State and local governments on preferred highway safety practices) focuses on traffic engineering services. The introduction outlines the purposes and objectives of Highway Safety Program Standard 13 and the Highway Safety Program Manual. Program development and…

Lunar mission safety and rescue: Escape/rescue analysis and plan

NASA Technical Reports Server (NTRS)

1971-01-01

The results are presented of the technical analysis of escape/rescue/survival situations, crew survival techniques, alternate escape/rescue approaches and vehicles, and the advantages and disadvantages of each for advanced lunar exploration. Candidate escape/rescue guidelines are proposed and elements of a rescue plan developed. The areas of discussions include the following: lunar arrival/departure operations, lunar orbiter operations, lunar surface operations, lunar surface base escape/rescue analysis, lander tug location operations, portable airlock, emergency pressure suit, and the effects of no orbiting lunar station, no lunar surface base, and no foreign lunar orbit/surface operations on the escape/rescue plan.

Behavioral analyses of wind-evoked escape of the cricket, Gryllodes sigillatus.

PubMed

Kanou, Masamichi; Konishi, Atsuko; Suenaga, Rie

2006-04-01

The wind-evoked escape behavior of the cricket Gryllodes sigillatus was investigated using an air puff stimulus. A high velocity air puff elicited the escape behavior in many crickets. The crickets tended to escape away from the stimulus source, but the direction was not accurately oriented 180 degrees from the stimulus. After bilateral cercal ablation, only a few crickets showed wind-evoked escape behavior, and their response rates did not increase even 19 days after ablation. Therefore, information on air motion detected by cercal filiform hairs is essential for triggering wind-evoked behavior. After unilateral cercal ablation, the 81.3% response rate of intact crickets decreased to 16.5%, that is, it decreased to almost 20% that of intact crickets. One week after unilateral cercal ablation, the response rate recovered to more than 60% that of intact crickets. However, the accuracy rate of the escape direction of G. sigillatus showed no change even immediately after the unilateral cercal ablation. Therefore, both cerci are not necessarily required to determine the escape direction. The behavioral characteristics of wind-evoked escape of G. sigillatus are compared with those of another species of cricket, Gryllus bimaculatus. The two species of cricket employ different strategies for wind-evoked escape.

Escapism among players of MMORPGs--conceptual clarification, its relation to mental health factors, and development of a new measure.

PubMed

Hagström, David; Kaldo, Viktor

2014-01-01

Previous studies show that the concept of escapism needs to be clarified and that its relation to problematic online gaming and other factors needs further examination. This study uses well-established, basic learning theory to clarify the concept of escapism, and examines its relation to problematic gaming, psychological distress, and satisfaction with life among players of massively multiplayer online role-playing games (MMORPGs). MMORPG players (n=201) answered an online questionnaire where these factors were measured and correlated with a previously developed scale on motivation to play (MTPI), including extra items to cover positive and negative aspects of escapism. Factor analysis and construct validation show that positive aspects of escapism are theoretically and empirically unstable and that escapism is best clarified as purely "negative escapism," corresponding to playing being negatively reinforced as a way of avoiding everyday hassles and distress. Negative escapism had a stronger relationship to symptoms of Internet addiction, psychological distress, and life satisfaction than other variables and other more positive motivations to play. Future studies should use the revised subscale for escapism (in the MTPI-R) presented in the present study, for example when screening for Internet addiction.

Ultrafast primary processes of an iron-(III) azido complex in solution induced with 266 nm light.

PubMed

Vennekate, Hendrik; Schwarzer, Dirk; Torres-Alacan, Joel; Krahe, Oliver; Filippou, Alexander C; Neese, Frank; Vöhringer, Peter

2012-05-14

The ultrafast photo-induced primary processes of the iron-(III) azido complex, [Fe(III)N(3)(cyclam-acetato)] PF(6) (1), in acetonitrile solution at room temperature were studied using femtosecond spectroscopy with ultraviolet (UV) excitation and mid-infrared (MIR) detection. Following the absorption of a 266 nm photon, the complex undergoes an internal conversion back to the electronic doublet ground state at a time scale below 2 ps. Subsequently, the electronic ground state vibrationally cools with a characteristic time constant of 13 ps. A homolytic bond cleavage was also observed by the appearance of ground state azide radicals, which were identified by their asymmetric stretching vibration at 1659 cm(-1). The azide radical recombines in a geminate fashion with the iron containing fragment within 20 ps. The cage escape leading to well separated fragments after homolytic Fe-N bond breakage was found to occur with a quantum yield of 35%. Finally, non-geminate recombination at nanosecond time scales was seen to further reduce the photolytic quantum yield to below 20% at a wavelength of 266 nm. This journal is © the Owner Societies 2012

13. Photograph of line drawing in possession of the Engineering ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

13. Photograph of line drawing in possession of the Engineering Division of the Directorate of Engineering and Housing, Watervliet Arsenal, New York. BRICK BAY FOR OFFICERS QUARTERS, BRICK SET, EAST SIDE, PLAN AND ELEVATION, OCTOBER 18, 1886. - Watervliet Arsenal, Building No. 4, Mordecai Drive, West of Mettler Road, Watervliet, Albany County, NY

Boiling Heat-Transfer Processes and Their Application in the Cooling of High Heat Flux Devices

DTIC Science & Technology

1993-06-01

1991, pp. 395-397. 385. Galloway, J. E. and Mudawar , 1. "Critical Heat Flux Enhancement by Means of Liquid Subcooling and Centrifugal Force Induced...Flow Boiling Heat Transfer for a Spirally Fluted Tube." Heat Tran~ler Engineering, Vol. 13, No.1, 1992, pp. 42-52. 390. Willingham, T. C. and Mudawar

High School/High Tech: Promoting Career Exploration Technology for Youth with Learning Disabilities and Behavioral Disorders.

ERIC Educational Resources Information Center

Lemaire, Gail Schoen; Mallik, Kalisankar; Stoll, Bryan G.

2002-01-01

A model program to promote science, engineering, and technology careers and address academic and vocational needs of low-income youth with learning disabilities includes referral and intake processes and academic and career training and services. Of the 21 first-year participants, 13 were employed (7 in technology-related positions) or enrolled in…

Sea Level Operation Demonstration of F404-GE-400 Turbofan Engine with JP-5/Bio-Fuel Mixture

DTIC Science & Technology

2010-03-30

Aircraft Test and Evaluation Facility Hush House at Naval Air Station Patuxent River, Maryland, on 13 October 2009. The test consisted of two separate...turbofan engine inside the Aircraft Test and Evaluation Facility Hush House at Naval Air Station Patuxent River, Maryland, on 13 October 2009. The test...turbofan engine (ESN 310810) inside the Aircraft Test and Evaluation Facility Hush House at Naval Air Station (NAS) Patuxent River, Maryland, on 13

Mars H Escape is potentially dominated by a high-altitude water source

NASA Astrophysics Data System (ADS)

Chaffin, Michael; Deighan, Justin; Schneider, Nick; Stewart, Ian

2017-04-01

H escape from the Mars atmosphere has removed a large part of Mars' initial water inventory. Until recently, this escape was thought to be slow and steady, sourced from long-lived molecular hydrogen whose lightness and volatility in comparison with water allow it to penetrate the upper atmosphere. Contradicting this thinking, observations from the Hubble Space Telescope and Mars Express, as well as more recent MAVEN measurements, indicate that H escape varies by at least a factor of ten over the Mars year and is largest in Southern Summer near perihelion. At the largest rates, H escape exceeds the ability of molecular hydrogen to supply the escape fluxes observed. At the same time in Southern Summer, Mars Express solar occultations have shown unexpectedly large concentrations of water at high altitude, potentially providing a source of escaping H unaccounted for in standard models. Here we show via photochemical modeling that the presence of this high altitude water can partially explain the large escape rates observed in Southern Summer. We further show that this escaping H is not in immediate balance with O escape, and therefore that short-term atmospheric dynamics can drive long-term variations in the oxidation balance and volatile content of planetary atmospheres. Future simultaneous observations by MAVEN, Mars Express, and the Trace Gas Orbiter may provide a direct test of this mechanism.

Elevated atmospheric escape of atomic hydrogen from Mars induced by high-altitude water

NASA Astrophysics Data System (ADS)

Chaffin, M. S.; Deighan, J.; Schneider, N. M.; Stewart, A. I. F.

2017-01-01

Atmospheric loss has controlled the history of Martian habitability, removing most of the planet’s initial water through atomic hydrogen and oxygen escape from the upper atmosphere to space. In standard models, H and O escape in a stoichiometric 2:1 ratio because H reaches the upper atmosphere via long-lived molecular hydrogen, whose abundance is regulated by a photochemical feedback sensitive to atmospheric oxygen content. The relatively constant escape rates these models predict are inconsistent with known H escape variations of more than an order of magnitude on seasonal timescales, variation that requires escaping H to have a source other than H2. The best candidate source is high-altitude water, detected by the Mars Express spacecraft in seasonally variable concentrations. Here we use a one-dimensional time-dependent photochemical model to show that the introduction of high-altitude water can produce a large increase in the H escape rate on a timescale of weeks, quantitatively linking these observations. This H escape pathway produces prompt H loss that is not immediately balanced by O escape, influencing the oxidation state of the atmosphere for millions of years. Martian atmospheric water loss may be dominated by escape via this pathway, which may therefore potentially control the planet’s atmospheric chemistry. Our findings highlight the influence that seasonal atmospheric variability can have on planetary evolution.

Sharks modulate their escape behavior in response to predator size, speed and approach orientation.

PubMed

Seamone, Scott; Blaine, Tristan; Higham, Timothy E

2014-12-01

Escape responses are often critical for surviving predator-prey interactions. Nevertheless, little is known about how predator size, speed and approach orientation impact escape performance, especially in larger prey that are primarily viewed as predators. We used realistic shark models to examine how altering predatory behavior and morphology (size, speed and approach orientation) influences escape behavior and performance in Squalus acanthias, a shark that is preyed upon by apex marine predators. Predator models induced C-start escape responses, and increasing the size and speed of the models triggered a more intense response (increased escape turning rate and acceleration). In addition, increased predator size resulted in greater responsiveness from the sharks. Among the responses, predator approach orientation had the most significant impact on escapes, such that the head-on approach, as compared to the tail-on approach, induced greater reaction distances and increased escape turning rate, speed and acceleration. Thus, the anterior binocular vision in sharks renders them less effective at detecting predators approaching from behind. However, it appears that sharks compensate by performing high-intensity escapes, likely induced by the lateral line system, or by a sudden visual flash of the predator entering their field of view. Our study reveals key aspects of escape behavior in sharks, highlighting the modulation of performance in response to predator approach. Copyright © 2014 Elsevier GmbH. All rights reserved.

Studies of Mechanisms of Pharmacological Enhancement of Functional Recovery After Cortical Contusion

DTIC Science & Technology

1993-01-29

ablation in rats. (Abstract). Presented at the MBRS Conference, November 3-5, Washington, D.C. 98. Levin, H.S., Benton, A.L. & Grossman, R.G. (1982...performance of contused rats during tests using a submerged escape platform (Figure 13). SWIM SPEED Z 12 0 Wo0 m SHAM0 BLOCK 1 0 CONTUSE W BLOCK I 09...recovery of beam walking and gait on a flat surface after sensorimotor cortex ablation in rats. (Abstract). Presented at the MBRS Conference, November 3

Determination of Pd, Pt and Rh in vehicles escape fumes by GF-AAS and ICP-OES.

PubMed

Goncalves, Antonio; Domínguez, José R; Alvarado, José

2008-04-15

Automotive exhaust gases from vehicles using catalytic converters were filtered through cellulose filter papers to collect suspended particles expulsed along with the engine's escape fumes. A specially designed sample collector was used for supporting the filter papers during collection. The collector was manufactured from a new car's exhaust pipe. A cellulose circular paper filter, 11 cm diameter, was attached to one end of the pipe and kept centered by pressing it against the borders of the pipe by means of a perforated aluminum cap, slightly wider than the pipe, used to cover this end of the collector. Filter papers loaded with the solid particles were acid-digested using a modified domestic microwave oven to bring the solid material into solution. The resulting solutions were analyzed for Pt by graphite furnace atomic absorption spectrometry (GF-AAS) and for Pd and Rh by inductively coupled plasma (ICP-OES). Results indicate that concentration of these analytes in the particulate is higher for new vehicles, having new catalytic converters, than for old ones. Maximum Pd, Pt and Rh in the samples analyzed were found to be 5.36, 12.60 and 1.03 microg g(-1), respectively.

Global relativistic effects in chaotic scattering.

PubMed

Bernal, Juan D; Seoane, Jesús M; Sanjuán, Miguel A F

2017-03-01

The phenomenon of chaotic scattering is very relevant in different fields of science and engineering. It has been mainly studied in the context of Newtonian mechanics, where the velocities of the particles are low in comparison with the speed of light. Here, we analyze global properties such as the escape time distribution and the decay law of the Hénon-Heiles system in the context of special relativity. Our results show that the average escape time decreases with increasing values of the relativistic factor β. As a matter of fact, we have found a crossover point for which the KAM islands in the phase space are destroyed when β≃0.4. On the other hand, the study of the survival probability of particles in the scattering region shows an algebraic decay for values of β≤0.4, and this law becomes exponential for β>0.4. Surprisingly, a scaling law between the exponent of the decay law and the β factor is uncovered where a quadratic fitting between them is found. The results of our numerical simulations agree faithfully with our qualitative arguments. We expect this work to be useful for a better understanding of both chaotic and relativistic systems.

The role of feedback in young people's academic choices

NASA Astrophysics Data System (ADS)

Skipper, Yvonne; Leman, Patrick J.

2017-03-01

Women are underrepresented in Science, Technology, Engineering and Mathematics subjects with more girls leaving these subjects at every stage in education. The current research used a scenario methodology to examine the impact of teacher feedback on girls' and boys' choices to study a specific science subject, engineering. British participants aged 13 (N = 479) were given scenarios where a new teacher encouraged them to take engineering using person feedback which focussed on their abilities, process feedback which focussed on their effort levels or gave them no reason. Results suggested that both boys and girls were more likely to select to study engineering when they received person feedback rather than process or no feedback. Young people also thought that ability was more important to being successful in science than in non-science subjects.This suggests young people feel that ability is needed to succeed in science subjects and person feedback can lead them to believe that they have this ability. Therefore, teacher feedback which gives ability attributions for possible success could be used to encourage more young people to persist in science. However, the potentially negative longer term outcomes of ability attributions and how they may be negated are also discussed.

Applications of Thin Film Thermocouples for Surface Temperature Measurement

NASA Technical Reports Server (NTRS)

Martin, Lisa C.; Holanda, Raymond

1994-01-01

Thin film thermocouples provide a minimally intrusive means of measuring surface temperature in hostile, high temperature environments. Unlike wire thermocouples, thin films do not necessitate any machining of the surface, therefore leaving intact its structural integrity. Thin films are many orders of magnitude thinner than wire, resulting in less disruption to the gas flow and thermal patterns that exist in the operating environment. Thin film thermocouples have been developed for surface temperature measurement on a variety of engine materials. The sensors are fabricated in the NASA Lewis Research Center's Thin Film Sensor Lab, which is a class 1000 clean room. The thermocouples are platinum-13 percent rhodium versus platinum and are fabricated by the sputtering process. Thin film-to-leadwire connections are made using the parallel-gap welding process. Thermocouples have been developed for use on superalloys, ceramics and ceramic composites, and intermetallics. Some applications of thin film thermocouples are: temperature measurement of space shuttle main engine turbine blade materials, temperature measurement in gas turbine engine testing of advanced materials, and temperature and heat flux measurements in a diesel engine. Fabrication of thin film thermocouples is described. Sensor durability, drift rate, and maximum temperature capabilities are addressed.

Evolution of the teachings of chemistry in the new degrees of School of Agricultural Engineering and its importance in the acquisition of competencies

NASA Astrophysics Data System (ADS)

Arce, Augusto; Tarquis, Ana M.; Castellanos, Maria Teresa; Requejo, Maria Isabel; Cartagena, Maria Carmen

2014-05-01

The academic year 2012-13 is the third year of implementation of the Bologna process in ETSI Agricultural for the subjects Chemistry I and Chemistry II in the new four Degrees: Graduate in Engineering and Agricultural Science, Food Engineering Graduate, Graduate in Engineering Environmental and Biotechnology graduate. We have implemented new interactive methodologies in the teaching-learning process based on the use of the virtual platform of the UPM, and teaching support materials and new laboratory practice developing has. It has also launched new continuous assessment systems that promote active student participation. A comparative study of academic achievements by students of the new grades in the subjects of chemistry during the last three academic years was performed to correlating the results obtained, the success rate and the drop out, and compare with the level of prior knowledge to those entering students. Possible solutions to try and fix these results in future courses are proposed Finally, the general competencies that contribute this course, how they are acquired and how they should be evaluated correctly are indicated. Acknowledgments: Innovation educative projects Nº IE02054-11/12 UPM. 2012

46 CFR 122.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

46 CFR 122.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

46 CFR 122.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 4 2014-10-01 2014-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

46 CFR 122.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

46 CFR 122.606 - Escape hatches and emergency exits.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Escape hatches and emergency exits. 122.606 Section 122.606 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING... Markings Required § 122.606 Escape hatches and emergency exits. All escape hatches and other emergency...

30 CFR 77.1101 - Escape and evacuation; plan.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Fire Protection § 77.1101 Escape and evacuation; plan. (a) Before September 30, 1971, each operator of... event of a fire. (b) All employees shall be instructed on current escape and evacuation plans, fire alarm signals, and applicable procedures to be followed in case of fire. (c) Plans for escape and...

30 CFR 77.1101 - Escape and evacuation; plan.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Fire Protection § 77.1101 Escape and evacuation; plan. (a) Before September 30, 1971, each operator of... event of a fire. (b) All employees shall be instructed on current escape and evacuation plans, fire alarm signals, and applicable procedures to be followed in case of fire. (c) Plans for escape and...