PC Scene Generation

NASA Astrophysics Data System (ADS)

Buford, James A., Jr.; Cosby, David; Bunfield, Dennis H.; Mayhall, Anthony J.; Trimble, Darian E.

2007-04-01

AMRDEC has successfully tested hardware and software for Real-Time Scene Generation for IR and SAL Sensors on COTS PC based hardware and video cards. AMRDEC personnel worked with nVidia and Concurrent Computer Corporation to develop a Scene Generation system capable of frame rates of at least 120Hz while frame locked to an external source (such as a missile seeker) with no dropped frames. Latency measurements and image validation were performed using COTS and in-house developed hardware and software. Software for the Scene Generation system was developed using OpenSceneGraph.

Certification of COTS Software in NASA Human Rated Flight Systems

NASA Technical Reports Server (NTRS)

Goforth, Andre

2012-01-01

Adoption of commercial off-the-shelf (COTS) products in safety critical systems has been seen as a promising acquisition strategy to improve mission affordability and, yet, has come with significant barriers and challenges. Attempts to integrate COTS software components into NASA human rated flight systems have been, for the most part, complicated by verification and validation (V&V) requirements necessary for flight certification per NASA s own standards. For software that is from COTS sources, and, in general from 3rd party sources, either commercial, government, modified or open source, the expectation is that it meets the same certification criteria as those used for in-house and that it does so as if it were built in-house. The latter is a critical and hidden issue. This paper examines the longstanding barriers and challenges in the use of 3rd party software in safety critical systems and cover recent efforts to use COTS software in NASA s Multi-Purpose Crew Vehicle (MPCV) project. It identifies some core artifacts that without them, the use of COTS and 3rd party software is, for all practical purposes, a nonstarter for affordable and timely insertion into flight critical systems. The paper covers the first use in a flight critical system by NASA of COTS software that has prior FAA certification heritage, which was shown to meet the RTCA-DO-178B standard, and how this certification may, in some cases, be leveraged to allow the use of analysis in lieu of testing. Finally, the paper proposes the establishment of an open source forum for development of safety critical 3rd party software.

Implementation plan for HANDI 2000 TWRS master equipment list

DOE Office of Scientific and Technical Information (OSTI.GOV)

BENNION, S.I.

This document presents the implementation plan for an additional deliverable of the HANDI 2000 Project. The PassPort Equipment Data module processes include those portions of the COTS PassPort system required to support tracking and management of the Master Equipment List for Lockheed Martin Hanford Company (LMHC) and custom software created to work with the COTS products.

Time-Domain Terahertz Computed Axial Tomography NDE System

NASA Technical Reports Server (NTRS)

Zimdars, David

2012-01-01

NASA has identified the need for advanced non-destructive evaluation (NDE) methods to characterize aging and durability in aircraft materials to improve the safety of the nation's airline fleet. 3D THz tomography can play a major role in detection and characterization of flaws and degradation in aircraft materials, including Kevlar-based composites and Kevlar and Zylon fabric covers for soft-shell fan containment where aging and durability issues are critical. A prototype computed tomography (CT) time-domain (TD) THz imaging system has been used to generate 3D images of several test objects including a TUFI tile (a thermal protection system tile used on the Space Shuttle and possibly the Orion or similar capsules). This TUFI tile had simulated impact damage that was located and the depth of damage determined. The CT motion control gan try was designed and constructed, and then integrated with a T-Ray 4000 control unit and motion controller to create a complete CT TD-THz imaging system prototype. A data collection software script was developed that takes multiple z-axis slices in sequence and saves the data for batch processing. The data collection software was integrated with the ability to batch process the slice data with the CT TD-THz image reconstruction software. The time required to take a single CT slice was decreased from six minutes to approximately one minute by replacing the 320 ps, 100-Hz waveform acquisition system with an 80 ps, 1,000-Hz waveform acquisition system. The TD-THZ computed tomography system was built from pre-existing commercial off-the-shelf subsystems. A CT motion control gantry was constructed from COTS components that can handle larger samples. The motion control gantry allows inspection of sample sizes of up to approximately one cubic foot (.0.03 cubic meters). The system reduced to practice a CT-TDTHz system incorporating a COTS 80- ps/l-kHz waveform scanner. The incorporation of this scanner in the system allows acquisition of 3D slice data with better signal-to-noise using a COTS scanner rather than the gchirped h scanner. The system also reduced to practice a prototype for commercial CT systems for insulating materials where safety concerns cannot accommodate x-ray. A software script was written to automate the COTS software to collect and process TD-THz CT data.

Assurance of COTS Boards for Space Flight. Part 1

NASA Technical Reports Server (NTRS)

Plante, Jeannette; Helmold, Norm; Eveland, Clay

1998-01-01

Space Flight hardware and software designers are increasingly turning to Commercial-Off-the-Shelf (COTS) products in hopes of meeting the demands imposed on them by projects with short development cycle times. The Technology Validation Assurance (TVA) team at NASA GSFC has embarked on applying a method for inserting COTS hardware into the Spartan 251 spacecraft. This method includes Procurement, Characterization, Ruggedization/Remediation and Verification Testing process steps which are intended to increase the uses confidence in the hardware's ability to function in the intended application for the required duration. As this method is refined with use, it has the potential for becoming a benchmark for industry-wide use of COTS in high reliability systems.

Generating Safety-Critical PLC Code From a High-Level Application Software Specification

NASA Technical Reports Server (NTRS)

2008-01-01

The benefits of automatic-application code generation are widely accepted within the software engineering community. These benefits include raised abstraction level of application programming, shorter product development time, lower maintenance costs, and increased code quality and consistency. Surprisingly, code generation concepts have not yet found wide acceptance and use in the field of programmable logic controller (PLC) software development. Software engineers at Kennedy Space Center recognized the need for PLC code generation while developing the new ground checkout and launch processing system, called the Launch Control System (LCS). Engineers developed a process and a prototype software tool that automatically translates a high-level representation or specification of application software into ladder logic that executes on a PLC. All the computer hardware in the LCS is planned to be commercial off the shelf (COTS), including industrial controllers or PLCs that are connected to the sensors and end items out in the field. Most of the software in LCS is also planned to be COTS, with only small adapter software modules that must be developed in order to interface between the various COTS software products. A domain-specific language (DSL) is a programming language designed to perform tasks and to solve problems in a particular domain, such as ground processing of launch vehicles. The LCS engineers created a DSL for developing test sequences of ground checkout and launch operations of future launch vehicle and spacecraft elements, and they are developing a tabular specification format that uses the DSL keywords and functions familiar to the ground and flight system users. The tabular specification format, or tabular spec, allows most ground and flight system users to document how the application software is intended to function and requires little or no software programming knowledge or experience. A small sample from a prototype tabular spec application is shown.

Workflow-Based Software Development Environment

NASA Technical Reports Server (NTRS)

Izygon, Michel E.

2013-01-01

The Software Developer's Assistant (SDA) helps software teams more efficiently and accurately conduct or execute software processes associated with NASA mission-critical software. SDA is a process enactment platform that guides software teams through project-specific standards, processes, and procedures. Software projects are decomposed into all of their required process steps or tasks, and each task is assigned to project personnel. SDA orchestrates the performance of work required to complete all process tasks in the correct sequence. The software then notifies team members when they may begin work on their assigned tasks and provides the tools, instructions, reference materials, and supportive artifacts that allow users to compliantly perform the work. A combination of technology components captures and enacts any software process use to support the software lifecycle. It creates an adaptive workflow environment that can be modified as needed. SDA achieves software process automation through a Business Process Management (BPM) approach to managing the software lifecycle for mission-critical projects. It contains five main parts: TieFlow (workflow engine), Business Rules (rules to alter process flow), Common Repository (storage for project artifacts, versions, history, schedules, etc.), SOA (interface to allow internal, GFE, or COTS tools integration), and the Web Portal Interface (collaborative web environment

Software Assurance: Five Essential Considerations for Acquisition Officials

DTIC Science & Technology

2007-05-01

May 2007 www.stsc.hill.af.mil 17 2 • address security concerns in the software development life cycle ( SDLC )? • Are there formal software quality...What threat modeling process, if any, is used when designing the software ? What analysis, design, and construction tools are used by your software design...the-shelf (COTS), government off-the-shelf (GOTS), open- source, embedded, and legacy software . Attackers exploit unintentional vulnerabil- ities or

A High Performance COTS Based Computer Architecture

NASA Astrophysics Data System (ADS)

Patte, Mathieu; Grimoldi, Raoul; Trautner, Roland

2014-08-01

Using Commercial Off The Shelf (COTS) electronic components for space applications is a long standing idea. Indeed the difference in processing performance and energy efficiency between radiation hardened components and COTS components is so important that COTS components are very attractive for use in mass and power constrained systems. However using COTS components in space is not straightforward as one must account with the effects of the space environment on the COTS components behavior. In the frame of the ESA funded activity called High Performance COTS Based Computer, Airbus Defense and Space and its subcontractor OHB CGS have developed and prototyped a versatile COTS based architecture for high performance processing. The rest of the paper is organized as follows: in a first section we will start by recapitulating the interests and constraints of using COTS components for space applications; then we will briefly describe existing fault mitigation architectures and present our solution for fault mitigation based on a component called the SmartIO; in the last part of the paper we will describe the prototyping activities executed during the HiP CBC project.

The Standard Autonomous File Server, A Customized, Off-the-Shelf Success Story

NASA Technical Reports Server (NTRS)

Semancik, Susan K.; Conger, Annette M.; Obenschain, Arthur F. (Technical Monitor)

2001-01-01

The Standard Autonomous File Server (SAFS), which includes both off-the-shelf hardware and software, uses an improved automated file transfer process to provide a quicker, more reliable, prioritized file distribution for customers of near real-time data without interfering with the assets involved in the acquisition and processing of the data. It operates as a stand-alone solution, monitoring itself, and providing an automated fail-over process to enhance reliability. This paper describes the unique problems and lessons learned both during the COTS selection and integration into SAFS, and the system's first year of operation in support of NASA's satellite ground network. COTS was the key factor in allowing the two-person development team to deploy systems in less than a year, meeting the required launch schedule. The SAFS system has been so successful; it is becoming a NASA standard resource, leading to its nomination for NASA's Software of the Year Award in 1999.

Robonaut's Flexible Information Technology Infrastructure

NASA Technical Reports Server (NTRS)

Askew, Scott; Bluethmann, William; Alder, Ken; Ambrose, Robert

2003-01-01

Robonaut, NASA's humanoid robot, is designed to work as both an astronaut assistant and, in certain situations, an astronaut surrogate. This highly dexterous robot performs complex tasks under telepresence control that could previously only be carried out directly by humans. Currently with 47 degrees of freedom (DOF), Robonaut is a state-of-the-art human size telemanipulator system. while many of Robonaut's embedded components have been custom designed to meet packaging or environmental requirements, the primary computing systems used in Robonaut are currently commercial-off-the-shelf (COTS) products which have some correlation to flight qualified computer systems. This loose coupling of information technology (IT) resources allows Robonaut to exploit cost effective solutions while floating the technology base to take advantage of the rapid pace of IT advances. These IT systems utilize a software development environment, which is both compatible with COTS hardware as well as flight proven computing systems, preserving the majority of software development for a flight system. The ability to use highly integrated and flexible COTS software development tools improves productivity while minimizing redesign for a space flight system. Further, the flexibility of Robonaut's software and communication architecture has allowed it to become a widely used distributed development testbed for integrating new capabilities and furthering experimental research.

Region and database management for HANDI 2000 business management system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, D.

The Data Integration 2000 Project will result in an integrated and comprehensive set of functional applications containing core information necessary to support the Project Hanford Management Contract. It is based on the Commercial-Off-The-Shelf product solution with commercially proven business processes. The COTS product solution set, of PassPort and People Soft software, supports finance, supply and chemical management/Material Safety Data Sheet, human resources.

Allocations for HANDI 2000 business management system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, D.

The Data Integration 2000 Project will result in an integrated and comprehensive set of functional applications containing core information necessary to support the Project Hanford Management Contract. It is based on the Commercial-Off-The-Shelf product solution with commercially proven business processes. The COTS product solution set, of PassPort and People Soft software, supports finance, supply and chemical management/Material Safety Data Sheet, human resources. Allocations at Fluor Daniel Hanford are burdens added to base costs using a predetermined rate.

A USNRC perspective on the use of commercial-off-shelf software (COTS) in advanced reactors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stewart, J.C.

1997-12-01

The use of commercially available digital computer systems and components in safety critical systems (nuclear power plant, military, and commercial applications) is increasing rapidly. While this paper focuses on the software aspects of the application most of these continents are applicable to the hardware aspects as well. Commercial dedication (the process of assuring that a commercial grade item will perform its intended safety function) has demonstrated benefits in cost savings and a wide base of user experience, however, care must be taken to avoid difficulties with some aspects of the dedication process such as access to vendor development information, configurationmore » management long term support, and system integration.« less

Incorporating Software Requirements into the System RFP: Survey of RFP Language for Software by Topic, v. 2.0

DTIC Science & Technology

2009-05-01

CDRL) System - Terminal S TMOS - SEIT CL TMOS - IA/ Crypto CL Space - Payload S Space - SEIT C Space - IA/ Crypto C Role Legend: CL...strategy for maintaining the currency of the technology (through Commercial off-the-shelf software (COTS) and other reusable Non-Developmental Items (NDI...the strategy for maintaining the currency of technology (e.g., through Commercial off-the-shelf software (COTS) insertion, technology refresh

Revealing the ISO/IEC 9126-1 Clique Tree for COTS Software Evaluation

NASA Technical Reports Server (NTRS)

Morris, A. Terry

2007-01-01

Previous research has shown that acyclic dependency models, if they exist, can be extracted from software quality standards and that these models can be used to assess software safety and product quality. In the case of commercial off-the-shelf (COTS) software, the extracted dependency model can be used in a probabilistic Bayesian network context for COTS software evaluation. Furthermore, while experts typically employ Bayesian networks to encode domain knowledge, secondary structures (clique trees) from Bayesian network graphs can be used to determine the probabilistic distribution of any software variable (attribute) using any clique that contains that variable. Secondary structures, therefore, provide insight into the fundamental nature of graphical networks. This paper will apply secondary structure calculations to reveal the clique tree of the acyclic dependency model extracted from the ISO/IEC 9126-1 software quality standard. Suggestions will be provided to describe how the clique tree may be exploited to aid efficient transformation of an evaluation model.

System integration test plan for HANDI 2000 business management system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, D.

This document presents the system integration test plan for the Commercial-Off-The-Shelf, PassPort and PeopleSoft software, and custom software created to work with the COTS products. The PP software is an integrated application for AP, Contract Management, Inventory Management, Purchasing and Material Safety Data Sheet. The PS software is an integrated application for Project Costing, General Ledger, Human Resources/Training, Payroll, and Base Benefits.

The Standard Autonomous File Server, a Customized, Off-the-Shelf Success Story

NASA Technical Reports Server (NTRS)

Semancik, Susan K.; Conger, Annette M.; Obenschain, Arthur F. (Technical Monitor)

2001-01-01

The Standard Autonomous File Server (SAFS), which includes both off-the-shelf hardware and software, uses an improved automated file transfer process to provide a quicker, more reliable, prioritized file distribution for customers of near real-time data without interfering with the assets involved in the acquisition and processing of the data. It operates as a stand-alone solution, monitoring itself, and providing an automated fail-over process to enhance reliability. This paper will describe the unique problems and lessons learned both during the COTS selection and integration into SAFS, and the system's first year of operation in support of NASA's satellite ground network. COTS was the key factor in allowing the two-person development team to deploy systems in less than a year, meeting the required launch schedule. The SAFS system his been so successful, it is becoming a NASA standard resource, leading to its nomination for NASA's Software or the Year Award in 1999.

Integrated Component-based Data Acquisition Systems for Aerospace Test Facilities

NASA Technical Reports Server (NTRS)

Ross, Richard W.

2001-01-01

The Multi-Instrument Integrated Data Acquisition System (MIIDAS), developed by the NASA Langley Research Center, uses commercial off the shelf (COTS) products, integrated with custom software, to provide a broad range of capabilities at a low cost throughout the system s entire life cycle. MIIDAS combines data acquisition capabilities with online and post-test data reduction computations. COTS products lower purchase and maintenance costs by reducing the level of effort required to meet system requirements. Object-oriented methods are used to enhance modularity, encourage reusability, and to promote adaptability, reducing software development costs. Using only COTS products and custom software supported on multiple platforms reduces the cost of porting the system to other platforms. The post-test data reduction capabilities of MIIDAS have been installed at four aerospace testing facilities at NASA Langley Research Center. The systems installed at these facilities provide a common user interface, reducing the training time required for personnel that work across multiple facilities. The techniques employed by MIIDAS enable NASA to build a system with a lower initial purchase price and reduced sustaining maintenance costs. With MIIDAS, NASA has built a highly flexible next generation data acquisition and reduction system for aerospace test facilities that meets customer expectations.

Analysis of Wallops Flight Test Data Through an Automated COTS System

NASA Technical Reports Server (NTRS)

Blackstock, Dexter Lee; Theobalds, Andre B.

2005-01-01

During the summer of 2004 NASA Langley Research Center flight tested a Synthetic Vision System (SVS) at the Reno/Tahoe International Airport (RNO) and the Wallops Flight Facility (WAL). The SVS included a Runway Incursion Prevention System (RIPS) to improve pilot situational awareness while operating near and on the airport surface. The flight tests consisted of air and ground operations to evaluate and validate the performance of the system. This paper describes the flight test and emphasizes how positioning data was collected, post processed and analyzed through the use of a COTS-derived software system. The system that was developed to analyze the data was constructed within the MATLAB(TM) environment. The software was modified to read the data, perform several if-then scenarios and produce the relevant graphs, figures and tables.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prescott, Ryan; Marger, Bernard L.; Chiu, Ailsa

During the second iteration of the US NDC Modernization Elaboration phase (E2), the SNL US NDC Modernization project team completed follow-on COTS surveys & exploratory prototyping related to the Object Storage & Distribution (OSD) mechanism, and the processing control software infrastructure. This report summarizes the E2 prototyping work.

Goal Structuring Notation in a Radiation Hardening Assurance Case for COTS-Based Spacecraft

NASA Technical Reports Server (NTRS)

Witulski, A.; Austin, R.; Evans, J.; Mahadevan, N.; Karsai, G.; Sierawski, B.; LaBel, K.; Reed, R.; Schrimpf, R.

2016-01-01

A systematic approach is presented to constructing a radiation assurance case using Goal Structuring Notation (GSN) for spacecraft containing COTS parts. The GSN paradigm is applied to an SRAM single-event upset experiment board designed to fly on a CubeSat in January 2017. A custom software language for development of a GSN assurance case is under development at Vanderbilt. Construction of a radiation assurance case without use of hardened parts or extensive radiation testing is discussed.

Applications Development for a Parallel COTS Spaceborne Computer

NASA Technical Reports Server (NTRS)

Katz, Daniel S.; Springer, Paul L.; Granat, Robert; Turmon, Michael

2000-01-01

This presentation reviews the Remote Exploration and Experimentation Project (REE) program for utilization of scalable supercomputing technology in space. The implementation of REE will be the use of COTS hardware and software to the maximum extent possible, keeping overhead low. Since COTS systems will be used, with little or no special modification, there will be significant cost reduction.

The Design of a Fault-Tolerant COTS-Based Bus Architecture

NASA Technical Reports Server (NTRS)

Chau, Savio N.; Alkalai, Leon; Burt, John B.; Tai, Ann T.

1999-01-01

In this paper, we report our experiences and findings on the design of a fault-tolerant bus architecture comprised of two COTS buses, the IEEE 1394 and the 12C. This fault-tolerant bus is the backbone system bus for the avionics architecture of the X2000 program at the Jet Propulsion Laboratory. COTS buses are attractive because of the availability of low cost commercial products. However, they are not specifically designed for highly reliable applications such as long-life deep-space missions. The X2000 design team has devised a multi-level fault tolerance approach to compensate for this shortcoming of COTS buses. First, the approach enhances the fault tolerance capabilities of the IEEE 1394 and 12 C buses by adding a layer of fault handling hardware and software. Second, algorithms are developed to enable the IEEE 1394 and the 12 C buses assist each other to isolate and recovery from faults. Third, the set of IEEE 1394 and 12 C buses is duplicated to further enhance system reliability. The X2000 design team has paid special attention to guarantee that all fault tolerance provisions will not cause the bus design to deviate from the commercial standard specifications. Otherwise, the economic attractiveness of using COTS will be diminished. The hardware and software design of the X2000 fault-tolerant bus are being implemented and flight hardware will be delivered to the ST4 and Europa Orbiter missions.

Space Shuttle Software Development and Certification

NASA Technical Reports Server (NTRS)

Orr, James K.; Henderson, Johnnie A

2000-01-01

Man-rated software, "software which is in control of systems and environments upon which human life is critically dependent," must be highly reliable. The Space Shuttle Primary Avionics Software System is an excellent example of such a software system. Lessons learn from more than 20 years of effort have identified basic elements that must be present to achieve this high degree of reliability. The elements include rigorous application of appropriate software development processes, use of trusted tools to support those processes, quantitative process management, and defect elimination and prevention. This presentation highlights methods used within the Space Shuttle project and raises questions that must be addressed to provide similar success in a cost effective manner on future long-term projects where key application development tools are COTS rather than internally developed custom application development tools

CSP: A Multifaceted Hybrid Architecture for Space Computing

NASA Technical Reports Server (NTRS)

Rudolph, Dylan; Wilson, Christopher; Stewart, Jacob; Gauvin, Patrick; George, Alan; Lam, Herman; Crum, Gary Alex; Wirthlin, Mike; Wilson, Alex; Stoddard, Aaron

2014-01-01

Research on the CHREC Space Processor (CSP) takes a multifaceted hybrid approach to embedded space computing. Working closely with the NASA Goddard SpaceCube team, researchers at the National Science Foundation (NSF) Center for High-Performance Reconfigurable Computing (CHREC) at the University of Florida and Brigham Young University are developing hybrid space computers that feature an innovative combination of three technologies: commercial-off-the-shelf (COTS) devices, radiation-hardened (RadHard) devices, and fault-tolerant computing. Modern COTS processors provide the utmost in performance and energy-efficiency but are susceptible to ionizing radiation in space, whereas RadHard processors are virtually immune to this radiation but are more expensive, larger, less energy-efficient, and generations behind in speed and functionality. By featuring COTS devices to perform the critical data processing, supported by simpler RadHard devices that monitor and manage the COTS devices, and augmented with novel uses of fault-tolerant hardware, software, information, and networking within and between COTS devices, the resulting system can maximize performance and reliability while minimizing energy consumption and cost. NASA Goddard has adopted the CSP concept and technology with plans underway to feature flight-ready CSP boards on two upcoming space missions.

A COTS RF Optical Software Defined Radio for the Integrated Radio and Optical Communications Test Bed

NASA Technical Reports Server (NTRS)

Nappier, Jennifer M.; Zeleznikar, Daniel J.; Wroblewski, Adam C.; Tokars, Roger P.; Schoenholz, Bryan L.; Lantz, Nicholas C.

2016-01-01

The Integrated Radio and Optical Communications (iROC) project at the National Aeronautics and Space Administration (NASA) is investigating the merits of a hybrid radio frequency (RF) and optical communication system for deep space missions. In an effort to demonstrate the feasibility and advantages of a hybrid RFOptical software defined radio (SDR), a laboratory prototype was assembled from primarily commercial-off-the-shelf (COTS) hardware components. This COTS platform has been used to demonstrate simultaneous transmission of the radio and optical communications waveforms through to the physical layer (telescope and antenna). This paper details the hardware and software used in the platform and various measures of its performance. A laboratory optical receiver platform has also been assembled in order to demonstrate hybrid free space links in combination with the transmitter.

A COTS RF/Optical Software Defined Radio for the Integrated Radio and Optical Communications Test Bed

NASA Technical Reports Server (NTRS)

Nappier, Jennifer M.; Zeleznikar, Daniel J.; Wroblewski, Adam C.; Tokars, Roger P.; Schoenholz, Bryan L.; Lantz, Nicholas C.

2017-01-01

The Integrated Radio and Optical Communications (iROC) project at the National Aeronautics and Space Administration (NASA) is investigating the merits of a hybrid radio frequency (RF) and optical communication system for deep space missions. In an effort to demonstrate the feasibility and advantages of a hybrid RF/Optical software defined radio (SDR), a laboratory prototype was assembled from primarily commercial-off-the-shelf (COTS) hardware components. This COTS platform has been used to demonstrate simultaneous transmission of the radio and optical communications waveforms through to the physical layer (telescope and antenna). This paper details the hardware and software used in the platform and various measures of its performance. A laboratory optical receiver platform has also been assembled in order to demonstrate hybrid free space links in combination with the transmitter.

High-efficiency space-based software radio architectures & algorithms (a minimum size, weight, and power TeraOps processor)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dunham, Mark Edward; Baker, Zachary K; Stettler, Matthew W

2009-01-01

Los Alamos has recently completed the latest in a series of Reconfigurable Software Radios, which incorporates several key innovations in both hardware design and algorithms. Due to our focus on satellite applications, each design must extract the best size, weight, and power performance possible from the ensemble of Commodity Off-the-Shelf (COTS) parts available at the time of design. In this case we have achieved 1 TeraOps/second signal processing on a 1920 Megabit/second datastream, while using only 53 Watts mains power, 5.5 kg, and 3 liters. This processing capability enables very advanced algorithms such as our wideband RF compression scheme tomore » operate remotely, allowing network bandwidth constrained applications to deliver previously unattainable performance.« less

Technical support plan for HANDI 2000 business management system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, D.E.

The Hanford Data Integration 2000 (HANDI 2000) Project will result in an integrated and comprehensive set of functional applications containing core information necessary to support the Project Hanford Management Contract. It is based on the Commercial-Off-The-Shelf (COTS) product solution with commercially proven business processes. The PassPort (PP) software is an integrated application for Accounts Payable, Contract Management, Inventory Management, and Purchasing. The PeopleSoft (PS) software is an integrated application for General Ledger, Project Costing, Human Resources, Payroll, Benefits, and Training. The implementation of this set of products, as the first deliverable of the HAND1 2000 Project, is referred to asmore » Business Management System (BMS) and Chemical Management.« less

Onboard Processor for Compressing HSI Data

NASA Technical Reports Server (NTRS)

Cook, Sid; Harsanyi, Joe; Day, John H. (Technical Monitor)

2002-01-01

With EO-1 Hyperion and MightySat in orbit NASA and the DoD are showing their continued commitment to hyperspectral imaging (HSI). As HSI sensor technology continues to mature, the ever-increasing amounts of sensor data generated will result in a need for more cost effective communication and data handling systems. Lockheed Martin, with considerable experience in spacecraft design and developing special purpose onboard processors, has teamed with Applied Signal & Image Technology (ASIT), who has an extensive heritage in HSI, to develop a real-time and intelligent onboard processing (OBP) system to reduce HSI sensor downlink requirements. Our goal is to reduce the downlink requirement by a factor greater than 100, while retaining the necessary spectral fidelity of the sensor data needed to satisfy the many science, military, and intelligence goals of these systems. Our initial spectral compression experiments leverage commercial-off-the-shelf (COTS) spectral exploitation algorithms for segmentation, material identification and spectral compression that ASIT has developed. ASIT will also support the modification and integration of this COTS software into the OBP. Other commercially available COTS software for spatial compression will also be employed as part of the overall compression processing sequence. Over the next year elements of a high-performance reconfigurable OBP will be developed to implement proven preprocessing steps that distill the HSI data stream in both spectral and spatial dimensions. The system will intelligently reduce the volume of data that must be stored, transmitted to the ground, and processed while minimizing the loss of information.

Three-dimensional Seismic Survey of the Continental-Ocean Transition Zone of the Northern South China Sea

NASA Astrophysics Data System (ADS)

Zhao, M.; Wang, Q.; Sibuet, J. C.; Sun, L.; Sun, Z.; Qiu, X.

2017-12-01

The South China Sea (SCS) is one of the largest marginal seas in the western Pacific, which has experienced extension, rifting, breakup, post-spreading magmatism on its northern margin during the Cenozoic era. The complexity of this margin is exacerbated by rifting and seafloor spreading processes, which developed at the expenses of the subducting proto-South China Sea. Based on Sun et al. (2014, 2016) proposals, 6 sites were drilled on the northern SCS margin from February to June 2017, during IODP Expeditions 367/368. The preliminary results indicate that the width of the COT is about 20 km and is different from the typical magma-poor Iberia margin whose width is around 100 km. The combination of three-dimensional (3D) Ocean Bottom Seismometers (OBS) refractive survey with IODP drilling results, will improve the drilling achievement and greatly contribute to the understanding of the specific mechanism of rifting and breakup processes of the northern SCS. In particular, it is expected to constrain: 1) the nature of the crust in the COT, 2) the degree of serpentinization of the upper mantle beneath the COT, and 3) the 3D extension of the COT, the oceanic crust and the serpentinized mantle. We firstly carry out the resolution tests and calculate the interval of OBSs using a ray tracing and travel time modelling software. 7-km interval between OBSs is the optimal interval for the resolution tests and ray coverage, which will provide optimal constraints for the characterization of the 20-km wide COT. The 3D seismic survey will be carried out in 2018. The design of the OBSs arrangement and the location of shooting lines are extremely important. At present, we propose 5 main profiles and 14 shooting lines along the multi-channel seismic lines already acquired in the vicinity of the 6 drilling sites. Any comments and suggestions concerning the OBSs arrangement will be appreciated. This work is supported by the Chinese National Natural Science Foundation (contracts 91428204, 41576070 and 41176053). Key words: Continental-ocean transition zone (COT); 3D refraction survey; IODP Expeditions 367/368; nature of crust.

"Making Do": Adapting Transaction Systems to Organizational Needs.

ERIC Educational Resources Information Center

Barreau, Deborah K.

2001-01-01

Discusses transaction systems that are designed for rapid data entry and task completion, and describes a study of three organizations that use commercial off-the-shelf software (COTS) products for providing customer service as well as for processing transactions. Concludes that organizations made do with systems designed for one application to…

Navigation and Elctro-Optic Sensor Integration Technology for Fusion of Imagery and Digital Mapping Products

DTIC Science & Technology

1999-08-01

Electro - Optic Sensor Integration Technology (NEOSIT) software application. The design is highly modular and based on COTS tools to facilitate integration with sensors, navigation and digital data sources already installed on different host

Library reuse in a rapid development environment

NASA Technical Reports Server (NTRS)

Uhde, JO; Weed, Daniel; Gottlieb, Robert; Neal, Douglas

1995-01-01

The Aeroscience and Flight Mechanics Division (AFMD) established a Rapid Development Laboratory (RDL) to investigate and improve new 'rapid development' software production processes and refine the use of commercial, off-the-shelf (COTS) tools. These tools and processes take an avionics design project from initial inception through high fidelity, real-time, hardware-in-the-loop (HIL) testing. One central theme of a rapid development process is the use and integration of a variety of COTS tools: This paper discusses the RDL MATRIX(sub x)(R) libraries, as well as the techniques for managing and documenting these libraries. This paper also shows the methods used for building simulations with the Advanced Simulation Development System (ASDS) libraries, and provides metrics to illustrate the amount of reuse for five complete simulations. Combining ASDS libraries with MATRIX(sub x)(R) libraries is discussed.

Web-Based Collaborative Publications System: R&Tserve

NASA Technical Reports Server (NTRS)

Abrams, Steve

1997-01-01

R&Tserve is a publications system based on 'commercial, off-the-shelf' (COTS) software that provides a persistent, collaborative workspace for authors and editors to support the entire publication development process from initial submission, through iterative editing in a hierarchical approval structure, and on to 'publication' on the WWW. It requires no specific knowledge of the WWW (beyond basic use) or HyperText Markup Language (HTML). Graphics and URLs are automatically supported. The system includes a transaction archive, a comments utility, help functionality, automated graphics conversion, automated table generation, and an email-based notification system. It may be configured and administered via the WWW and can support publications ranging from single page documents to multiple-volume 'tomes'.

Accruals for HANDI 2000 business management system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, D.

The Data Integration 2000 Project will result in an integrated and comprehensive set of functional applications containing core information necessary to support the Project Hanford Management Contract. It is based on the Commercial-Off-The-Shelf product solution with commercially proven business processes. The COTS product solution set, of PassPort and People Soft software, supports finance, supply and chemical management/Material Safety Data Sheet, human resources. Accruals are made at the project level. At the inception of each project, Project Management and the Accounts Payable Group make a mutual decision on whether periodic accrual entries should be made for it.

Telescience Resource Kit

NASA Technical Reports Server (NTRS)

Schneider, Michelle; Lippincott, Jeff; Chubb, Steve; Whitaker, Jimmy; Rice, Jim; Gillis, Robert; Sims, Chris; Sellers, Donna; Bailey, Darrell (Technical Monitor)

2002-01-01

The Telescience Resource Kit (TReK) is a PC based ground control system. It can be used by a single individual or in a group environment to monitor and control spacecraft systems and payloads. Capabilities include data receipt, data processing, data storage, data management, and data transmission. Commercial-Off-The-Shelf (COTS) hardware and software have been employed to reduce development costs, operations and maintenance costs, and to effectively take advantage of new commercial products as they become available. The TReK system is currently being used to monitor and control payloads aboard the International Space Station. It is located at sites around the world.

Issues and Methods for Assessing COTS Reliability, Maintainability, and Availability

NASA Technical Reports Server (NTRS)

Schneidewind, Norman F.; Nikora, Allen P.

1998-01-01

Many vendors produce products that are not domain specific (e.g., network server) and have limited functionality (e.g., mobile phone). In contrast, many customers of COTS develop systems that am domain specific (e.g., target tracking system) and have great variability in functionality (e.g., corporate information system). This discussion takes the viewpoint of how the customer can ensure the quality of COTS components. In evaluating the benefits and costs of using COTS, we must consider the environment in which COTS will operate. Thus we must distinguish between using a non-mission critical application like a spreadsheet program to produce a budget and a mission critical application like military strategic and tactical operations. Whereas customers will tolerate an occasional bug in the former, zero tolerance is the rule in the latter. We emphasize the latter because this is the arena where there are major unresolved problems in the application of COTS. Furthermore, COTS components may be embedded in the larger customer system. We refer to these as embedded systems. These components must be reliable, maintainable, and available, and must be with the larger system in order for the customer to benefit from the advertised advantages of lower development and maintenance costs. Interestingly, when the claims of COTS advantages are closely examined, one finds that to a great extent these COTS components consist of hardware and office products, not mission critical software [1]. Obviously, COTS components are different from custom components with respect to one or more of the following attributes: source, development paradigm, safety, reliability, maintainability, availability, security, and other attributes. However, the important question is whether they should be treated differently when deciding to deploy them for operational use; we suggest the answer is no. We use reliability as an example to justify our answer. In order to demonstrate its reliability, a COTS component must pass the same reliability evaluations as the custom components, otherwise the COTS components will be the weakest link in the chain of components and will be the determinant of software system reliability. The challenge is that there will be less information available for evaluating COTS components than for custom components but this does not mean we should despair and do nothing. Actually, there is a lot we can do even in the absence of documentation on COTS components because the customer will have information about how COTS components are to be used in the larger system. To illustrate our approach, we will consider the reliability, maintainability, and availability (RMA) of COTS components as used in larger systems. Finally, COTS suppliers might consider increasing visibility into their products to assist customers in determining the components' fitness for use in a particular application. We offer ideas of information that would be useful to customers, and what vendors might do to provide it.

C3I Systems Acquisition and Maintenance in Relation to the use of COTS Products

DTIC Science & Technology

2000-12-01

the NATO C3 Agency and crypto equipment. * the GFE STARGATE Software subsystem (the prototyped version of which, developed by IAF, Surveillance fctid...been increasing and dual-use systems (ACCAM, ICC, AOIS, STARGATE and re-use potentials have been enhanced. WAN connections) Use of COTS information

Reconfigurable, Cognitive Software-Defined Radio

NASA Technical Reports Server (NTRS)

Bhat, Arvind

2015-01-01

Software-defined radio (SDR) technology allows radios to be reconfigured to perform different communication functions without using multiple radios to accomplish each task. Intelligent Automation, Inc., has developed SDR platforms that switch adaptively between different operation modes. The innovation works by modifying both transmit waveforms and receiver signal processing tasks. In Phase I of the project, the company developed SDR cognitive capabilities, including adaptive modulation and coding (AMC), automatic modulation recognition (AMR), and spectrum sensing. In Phase II, these capabilities were integrated into SDR platforms. The reconfigurable transceiver design employs high-speed field-programmable gate arrays, enabling multimode operation and scalable architecture. Designs are based on commercial off-the-shelf (COTS) components and are modular in nature, making it easier to upgrade individual components rather than redesigning the entire SDR platform as technology advances.

SAO mission support software and data standards, version 1.0

NASA Technical Reports Server (NTRS)

Hsieh, P.

1993-01-01

This document defines the software developed by the SAO AXAF Mission Support (MS) Program and defines standards for the software development process and control of data products generated by the software. The SAO MS is tasked to develop and use software to perform a variety of functions in support of the AXAF mission. Software is developed by software engineers and scientists, and commercial off-the-shelf (COTS) software is used either directly or customized through the use of scripts to implement analysis procedures. Software controls real-time laboratory instruments, performs data archiving, displays data, and generates model predictions. Much software is used in the analysis of data to generate data products that are required by the AXAF project, for example, on-orbit mirror performance predictions or detailed characterization of the mirror reflection performance with energy.

Software architecture of the III/FBI segment of the FBI's integrated automated identification system

NASA Astrophysics Data System (ADS)

Booker, Brian T.

1997-02-01

This paper will describe the software architecture of the Interstate Identification Index (III/FBI) Segment of the FBI's Integrated Automated Fingerprint Identification System (IAFIS). IAFIS is currently under development, with deployment to begin in 1998. III/FBI will provide the repository of criminal history and photographs for criminal subjects, as well as identification data for military and civilian federal employees. Services provided by III/FBI include maintenance of the criminal and civil data, subject search of the criminal and civil data, and response generation services for IAFIS. III/FBI software will be comprised of both COTS and an estimated 250,000 lines of developed C code. This paper will describe the following: (1) the high-level requirements of the III/FBI software; (2) the decomposition of the III/FBI software into Computer Software Configuration Items (CSCIs); (3) the top-level design of the III/FBI CSCIs; and (4) the relationships among the developed CSCIs and the COTS products that will comprise the III/FBI software.

Managing configuration software of ground software applications with glueware

NASA Technical Reports Server (NTRS)

Larsen, B.; Herrera, R.; Sesplaukis, T.; Cheng, L.; Sarrel, M.

2003-01-01

This paper reports on a simple, low-cost effort to streamline the configuration of the uplink software tools. Even though the existing ground system consisted of JPL and custom Cassini software rather than COTS, we chose a glueware approach--reintegrating with wrappers and bridges and adding minimal new functionality.

The Chandra X-ray Center data system: supporting the mission of the Chandra X-ray Observatory

NASA Astrophysics Data System (ADS)

Evans, Janet D.; Cresitello-Dittmar, Mark; Doe, Stephen; Evans, Ian; Fabbiano, Giuseppina; Germain, Gregg; Glotfelty, Kenny; Hall, Diane; Plummer, David; Zografou, Panagoula

2006-06-01

The Chandra X-ray Center Data System provides end-to-end scientific software support for Chandra X-ray Observatory mission operations. The data system includes the following components: (1) observers' science proposal planning tools; (2) science mission planning tools; (3) science data processing, monitoring, and trending pipelines and tools; and (4) data archive and database management. A subset of the science data processing component is ported to multiple platforms and distributed to end-users as a portable data analysis package. Web-based user tools are also available for data archive search and retrieval. We describe the overall architecture of the data system and its component pieces, and consider the design choices and their impacts on maintainability. We discuss the many challenges involved in maintaining a large, mission-critical software system with limited resources. These challenges include managing continually changing software requirements and ensuring the integrity of the data system and resulting data products while being highly responsive to the needs of the project. We describe our use of COTS and OTS software at the subsystem and component levels, our methods for managing multiple release builds, and adapting a large code base to new hardware and software platforms. We review our experiences during the life of the mission so-far, and our approaches for keeping a small, but highly talented, development team engaged during the maintenance phase of a mission.

GERICOS: A Generic Framework for the Development of On-Board Software

NASA Astrophysics Data System (ADS)

Plasson, P.; Cuomo, C.; Gabriel, G.; Gauthier, N.; Gueguen, L.; Malac-Allain, L.

2016-08-01

This paper presents an overview of the GERICOS framework (GEneRIC Onboard Software), its architecture, its various layers and its future evolutions. The GERICOS framework, developed and qualified by LESIA, offers a set of generic, reusable and customizable software components for the rapid development of payload flight software. The GERICOS framework has a layered structure. The first layer (GERICOS::CORE) implements the concept of active objects and forms an abstraction layer over the top of real-time kernels. The second layer (GERICOS::BLOCKS) offers a set of reusable software components for building flight software based on generic solutions to recurrent functionalities. The third layer (GERICOS::DRIVERS) implements software drivers for several COTS IP cores of the LEON processor ecosystem.

Lessons Learned on Implementing Fault Detection, Isolation, and Recovery (FDIR) in a Ground Launch Environment

NASA Technical Reports Server (NTRS)

Ferrell, Bob A.; Lewis, Mark E.; Perotti, Jose M.; Brown, Barbara L.; Oostdyk, Rebecca L.; Goetz, Jesse W.

2010-01-01

This paper's main purpose is to detail issues and lessons learned regarding designing, integrating, and implementing Fault Detection Isolation and Recovery (FDIR) for Constellation Exploration Program (CxP) Ground Operations at Kennedy Space Center (KSC). Part of the0 overall implementation of National Aeronautics and Space Administration's (NASA's) CxP, FDIR is being implemented in three main components of the program (Ares, Orion, and Ground Operations/Processing). While not initially part of the design baseline for the CxP Ground Operations, NASA felt that FDIR is important enough to develop, that NASA's Exploration Systems Mission Directorate's (ESMD's) Exploration Technology Development Program (ETDP) initiated a task for it under their Integrated System Health Management (ISHM) research area. This task, referred to as the FDIIR project, is a multi-year multi-center effort. The primary purpose of the FDIR project is to develop a prototype and pathway upon which Fault Detection and Isolation (FDI) may be transitioned into the Ground Operations baseline. Currently, Qualtech Systems Inc (QSI) Commercial Off The Shelf (COTS) software products Testability Engineering and Maintenance System (TEAMS) Designer and TEAMS RDS/RT are being utilized in the implementation of FDI within the FDIR project. The TEAMS Designer COTS software product is being utilized to model the system with Functional Fault Models (FFMs). A limited set of systems in Ground Operations are being modeled by the FDIR project, and the entire Ares Launch Vehicle is being modeled under the Functional Fault Analysis (FFA) project at Marshall Space Flight Center (MSFC). Integration of the Ares FFMs and the Ground Processing FFMs is being done under the FDIR project also utilizing the TEAMS Designer COTS software product. One of the most significant challenges related to integration is to ensure that FFMs developed by different organizations can be integrated easily and without errors. Software Interface Control Documents (ICDs) for the FFMs and their usage will be addressed as the solution to this issue. In particular, the advantages and disadvantages of these ICDs across physically separate development groups will be delineated.

PC based PLCs and ethernet based fieldbus: the new standard platform for future VLT instrument control

NASA Astrophysics Data System (ADS)

Kiekebusch, Mario J.; Lucuix, Christian; Erm, Toomas M.; Chiozzi, Gianluca; Zamparelli, Michele; Kern, Lothar; Brast, Roland; Pirani, Werther; Reiss, Roland; Popovic, Dan; Knudstrup, Jens; Duchateau, Michel; Sandrock, Stefan; Di Lieto, Nicola

2014-07-01

ESO is currently in the final phase of the standardization process for PC-based Programmable Logical Controllers (PLCs) as the new platform for the development of control systems for future VLT/VLTI instruments. The standard solution used until now consists of a Local Control Unit (LCU), a VME-based system having a CPU and commercial and proprietary boards. This system includes several layers of software and many thousands of lines of code developed and maintained in house. LCUs have been used for several years as the interface to control instrument functions but now are being replaced by commercial off-the-shelf (COTS) systems based on BECKHOFF Embedded PCs and the EtherCAT fieldbus. ESO is working on the completion of the software framework that enables a seamless integration into the VLT control system in order to be ready to support upcoming instruments like ESPRESSO and ERIS, that will be the first fully VLT compliant instruments using the new standard. The technology evaluation and standardization process has been a long and combined effort of various engineering disciplines like electronics, control and software, working together to define a solution that meets the requirements and minimizes the impact on the observatory operations and maintenance. This paper presents the challenges of the standardization process and the steps involved in such a change. It provides a technical overview of how industrial standards like EtherCAT, OPC-UA, PLCOpen MC and TwinCAT can be used to replace LCU features in various areas like software engineering and programming languages, motion control, time synchronization and astronomical tracking.

Missile signal processing common computer architecture for rapid technology upgrade

NASA Astrophysics Data System (ADS)

Rabinkin, Daniel V.; Rutledge, Edward; Monticciolo, Paul

2004-10-01

Interceptor missiles process IR images to locate an intended target and guide the interceptor towards it. Signal processing requirements have increased as the sensor bandwidth increases and interceptors operate against more sophisticated targets. A typical interceptor signal processing chain is comprised of two parts. Front-end video processing operates on all pixels of the image and performs such operations as non-uniformity correction (NUC), image stabilization, frame integration and detection. Back-end target processing, which tracks and classifies targets detected in the image, performs such algorithms as Kalman tracking, spectral feature extraction and target discrimination. In the past, video processing was implemented using ASIC components or FPGAs because computation requirements exceeded the throughput of general-purpose processors. Target processing was performed using hybrid architectures that included ASICs, DSPs and general-purpose processors. The resulting systems tended to be function-specific, and required custom software development. They were developed using non-integrated toolsets and test equipment was developed along with the processor platform. The lifespan of a system utilizing the signal processing platform often spans decades, while the specialized nature of processor hardware and software makes it difficult and costly to upgrade. As a result, the signal processing systems often run on outdated technology, algorithms are difficult to update, and system effectiveness is impaired by the inability to rapidly respond to new threats. A new design approach is made possible three developments; Moore's Law - driven improvement in computational throughput; a newly introduced vector computing capability in general purpose processors; and a modern set of open interface software standards. Today's multiprocessor commercial-off-the-shelf (COTS) platforms have sufficient throughput to support interceptor signal processing requirements. This application may be programmed under existing real-time operating systems using parallel processing software libraries, resulting in highly portable code that can be rapidly migrated to new platforms as processor technology evolves. Use of standardized development tools and 3rd party software upgrades are enabled as well as rapid upgrade of processing components as improved algorithms are developed. The resulting weapon system will have a superior processing capability over a custom approach at the time of deployment as a result of a shorter development cycles and use of newer technology. The signal processing computer may be upgraded over the lifecycle of the weapon system, and can migrate between weapon system variants enabled by modification simplicity. This paper presents a reference design using the new approach that utilizes an Altivec PowerPC parallel COTS platform. It uses a VxWorks-based real-time operating system (RTOS), and application code developed using an efficient parallel vector library (PVL). A quantification of computing requirements and demonstration of interceptor algorithm operating on this real-time platform are provided.

Operations system administration plan for HANDI 2000 business management system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, D.E.

The Hanford Data Integration 2000 (HANDI 2000) Project will result in an integrated and comprehensive set of functional applications containing core information necessary to support the Project Hanford Management Contract (PHMC). It is based on the Commercial-Off-The-Shelf (COTS) product solution with commercially proven business processes. This includes systems that support finance, supply, chemical management, human resources and payroll activities on the Hanford Site. The Passport (PP) software is an integrated application for Accounts Payable, Contract Management, Inventory Management, Purchasing, and Material Safety Data Sheets (MSDS). The PeopleSoft (PS) software is an integrated application for General Ledger, Project Costing, Human Resources,more » Payroll, Benefits, and Training. The implementation of this set of products, as the first deliverable of the HANDI 2000 Project, is referred to as Business Management System (BMS) and MSDS.« less

Training the Next Generation in Space Situational Awareness Research

NASA Astrophysics Data System (ADS)

Colpo, D.; Reddy, V.; Arora, S.; Tucker, S.; Jeffries, L.; May, D.; Bronson, R.; Hunten, E.

Traditional academic SSA research has relied on commercial off the shelf (COTS) systems for collecting metric and lightcurve data. COTS systems have several advantages over a custom built system including cost, easy integration, technical support and short deployment timescales. We at the University of Arizona took an alternative approach to develop a sensor system for space object characterization. Five engineering students designed and built two 0.6-meter F/4 electro-optical (EO) systems for collecting lightcurve and spectral data. All the design and fabrication work was carried out over the course of two semesters as part f their senior design project that is mandatory for the completion of their bachelors in engineering degree. The students designed over 200 individual parts using three-dimensional modeling software (SolidWorks), and conducted detailed optical design analysis using raytracing software (ZEMAX), with oversight and advice from faculty sponsor and Starizona, a local small business in Tucson. The components of the design were verified by test, analysis, inspection, or demonstration, per the process that the University of Arizona requires for each of its design projects. Methods to complete this project include mechanical FEA, optical testing methods (Foucault Knife Edge Test and Couder Mask Test), tests to verify the function of the thermometers, and a final pointing model test. A surprise outcome of our exercise is that the entire cost of the design and fabrication of these two EO systems was significantly lower than a COTS alternative. With careful planning and coordination we were also able to reduce to the deployment times to those for a commercial system. Our experience shows that development of hardware and software for SSA research could be accomplished in an academic environment that would enable the training of the next generation with active support from local small businesses.

Development of Secondary Archive System at Goddard Space Flight Center Version 0 Distributed Active Archive Center

NASA Technical Reports Server (NTRS)

Sherman, Mark; Kodis, John; Bedet, Jean-Jacques; Wacker, Chris; Woytek, Joanne; Lynnes, Chris

1996-01-01

The Goddard Space Flight Center (GSFC) version 0 Distributed Active Archive Center (DAAC) has been developed to support existing and pre Earth Observing System (EOS) Earth science datasets, facilitate the scientific research, and test EOS data and information system (EOSDIS) concepts. To ensure that no data is ever lost, each product received at GSFC DAAC is archived on two different media, VHS and digital linear tape (DLT). The first copy is made on VHS tape and is under the control of UniTree. The second and third copies are made to DLT and VHS media under a custom built software package named 'Archer'. While Archer provides only a subset of the functions available with commercial software like UniTree, it supports migration between near-line and off-line media and offers much greater performance and flexibility to satisfy the specific needs of a data center. Archer is specifically designed to maximize total system throughput, rather than focusing on the turn-around time for individual files. The commercial off the shelf software (COTS) hierarchical storage management (HSM) products evaluated were mainly concerned with transparent, interactive, file access to the end-user, rather than a batch-orientated, optimizable (based on known data file characteristics) data archive and retrieval system. This is critical to the distribution requirements of the GSFC DAAC where orders for 5000 or more files at a time are received. Archer has the ability to queue many thousands of file requests and to sort these requests into internal processing schedules that optimize overall throughput. Specifically, mount and dismount, tape load and unload cycles, and tape motion are minimized. This feature did not seem to be available in many COTS pacages. Archer also uses a generic tar tape format that allows tapes to be read by many different systems rather than the proprietary format found in most COTS packages. This paper discusses some of the specific requirements at GSFC DAAC, the motivations for implementing the Archer system, and presents a discussion of the Archer design that resulted.

Experimental evaluation of a COTS system for space applications

NASA Technical Reports Server (NTRS)

Some, R. R.; Madeira, H.; Moreira, F.; Costa, D.; Rennels, D.

2002-01-01

The use of COTS-based systems in space missions for scientific data processing is very attractive, as their ratio of performance to power consumption of commercial components can be an order of magnitude greater than that of radiation hardened components, and the price differential is even higher.

Development of wide band digital receiver for atmospheric radars using COTS board based SDR

NASA Astrophysics Data System (ADS)

Yasodha, Polisetti; Jayaraman, Achuthan; Thriveni, A.

2016-07-01

Digital receiver extracts the received echo signal information, and is a potential subsystem for atmospheric radar, also referred to as wind profiling radar (WPR), which provides the vertical profiles of 3-dimensional wind vector in the atmosphere. This paper presents the development of digital receiver using COTS board based Software Defined Radio technique, which can be used for atmospheric radars. The developmental work is being carried out at National Atmospheric Research Laboratory (NARL), Gadanki. The digital receiver consists of a commercially available software defined radio (SDR) board called as universal software radio peripheral B210 (USRP B210) and a personal computer. USRP B210 operates over a wider frequency range from 70 MHz to 6 GHz and hence can be used for variety of radars like Doppler weather radars operating in S/C bands, in addition to wind profiling radars operating in VHF, UHF and L bands. Due to the flexibility and re-configurability of SDR, where the component functionalities are implemented in software, it is easy to modify the software to receive the echoes and process them as per the requirement suitable for the type of the radar intended. Hence, USRP B210 board along with the computer forms a versatile digital receiver from 70 MHz to 6 GHz. It has an inbuilt direct conversion transceiver with two transmit and two receive channels, which can be operated in fully coherent 2x2 MIMO fashion and thus it can be used as a two channel receiver. Multiple USRP B210 boards can be synchronized using the pulse per second (PPS) input provided on the board, to configure multi-channel digital receiver system. RF gain of the transceiver can be varied from 0 to 70 dB. The board can be controlled from the computer via USB 3.0 interface through USRP hardware driver (UHD), which is an open source cross platform driver. The USRP B210 board is connected to the personal computer through USB 3.0. Reference (10 MHz) clock signal from the radar master oscillator is used to lock the board, which is essential for deriving Doppler information. Input from the radar analog receiver is given to one channel of USRP B210, which is down converted to baseband. 12-bit ADC present on the board digitizes the signal and produces I (in-phase) and Q (quadrature-phase) data. The maximum sampling rate possible is about 61 MSPS. The I and Q (time series) data is sent to PC via USB 3.0, where the signal processing is carried out. The online processing steps include decimation, range gating, decoding, coherent integration and FFT computation (optional). The processed data is then stored in the hard disk. C++ programming language is used for developing the real time signal processing. Shared memory along with multi threading is used to collect and process data simultaneously. Before implementing the real time operation, stand alone test of the board was carried out through GNU radio software and the base band output data obtained is found satisfactory. Later the board is integrated with the existing Lower Atmospheric Wind Profiling radar at NARL. The radar receive IF output at 70 MHz is given to the board and the real-time radar data is collected. The data is processed off-line and the range-doppler spectrum is obtained. Online processing software is under progress.

Goddard Space Flight Center's Structural Dynamics Data Acquisition System

NASA Technical Reports Server (NTRS)

McLeod, Christopher

2004-01-01

Turnkey Commercial Off The Shelf (COTS) data acquisition systems typically perform well and meet most of the objectives of the manufacturer. The problem is that they seldom meet most of the objectives of the end user. The analysis software, if any, is unlikely to be tailored to the end users specific application; and there is seldom the chance of incorporating preferred algorithms to solve unique problems. Purchasing a customized system allows the end user to get a system tailored to the actual application, but the cost can be prohibitive. Once the system has been accepted, future changes come with a cost and response time that's often not workable. When it came time to replace the primary digital data acquisition system used in the Goddard Space Flight Center's Structural Dynamics Test Section, the decision was made to use a combination of COTS hardware and in-house developed software. The COTS hardware used is the DataMAX II Instrumentation Recorder built by R.C. Electronics Inc. and a desktop Pentium 4 computer system. The in-house software was developed using MATLAB from The MathWorks. This paper will describe the design and development of the new data acquisition and analysis system.

Goddard Space Flight Center's Structural Dynamics Data Acquisition System

NASA Technical Reports Server (NTRS)

McLeod, Christopher

2004-01-01

Turnkey Commercial Off The Shelf (COTS) data acquisition systems typically perform well and meet most of the objectives of the manufacturer. The problem is that they seldom meet most of the objectives of the end user. The analysis software, if any, is unlikely to be tailored to the end users specific application; and there is seldom the chance of incorporating preferred algorithms to solve unique problems. Purchasing a customized system allows the end user to get a system tailored to the actual application, but the cost can be prohibitive. Once the system has been accepted, future changes come with a cost and response time that's often not workable. When it came time to replace the primary digital data acquisition system used in the Goddard Space Flight Center's Structural Dynamics Test Section, the decision was made to use a combination of COTS hardware and in-house developed software. The COTS hardware used is the DataMAX II Instrumentation Recorder built by R.C. Electronics Inc. and a desktop Pentium 4 computer system. The in-house software was developed using MATLAF3 from The Mathworks. This paper will describe the design and development of the new data acquisition and analysis system.

Six Facets of the Open COTS Box

DTIC Science & Technology

2000-03-01

customisation of the presentation the package affect me and what interface. Typically, though, they will be used for dependencies does it create? different...types of applications. COTS software is fundamentally oriented to a The approach that will leave the greatest mass market. New releases can serve a number...flexibility for customisation of the look of the of purposes in this environment: presentation interface by individuals will be the ° They are a

The Integration of COTS/GOTS within NASA's HST Command and Control System

NASA Technical Reports Server (NTRS)

Pfarr, Thomas; Reis, James E.; Obenschain, Arthur F. (Technical Monitor)

2001-01-01

NASA's mission critical Hubble Space Telescope (HST) command and control system has been re-engineered with COTS/GOTS and minimal custom code. This paper focuses on the design of this new HST Control Center System (CCS) and the lessons learned throughout its development. CCS currently utilizes 31 COTS/GOTS products with an additional 12 million lines of custom glueware code; the new CCS exceeds the capabilities of the original system while significantly reducing the lines of custom code by more than 50%. The lifecycle of COTS/GOTS products will be examined including the pack-age selection process, evaluation process, and integration process. The advantages, disadvantages, issues, concerns, and lessons teamed for integrating COTS/GOTS into the NASA's mission critical HST CCS will be examined in detail. Command and control systems designed with traditional custom code development efforts will be compared with command and control systems designed with new development techniques relying heavily on COTS/COTS integration. This paper will reveal the many hidden costs of COTS/GOTS solutions when compared to traditional custom code development efforts; this paper will show the high cost of COTS/GOTS solutions including training expenses, consulting fees, and long-term maintenance expenses.

Survey Software Evaluation

DTIC Science & Technology

2009-01-01

Oracle 9i, 10g  MySQL  MS SQL Server MS SQL Server Operating System Supported Windows 2003 Server  Windows 2000 Server (32 bit...WebStar (Mac OS X)  SunOne Internet Information Services (IIS) Database Server Supported MS SQL Server  MS SQL Server  Oracle 9i, 10g...challenges of Web-based surveys are: 1) identifying the best Commercial Off the Shelf (COTS) Web-based survey packages to serve the particular

A "Kane's Dynamics" Model for the Active Rack Isolation System Part Two: Nonlinear Model Development, Verification, and Simplification

NASA Technical Reports Server (NTRS)

Beech, G. S.; Hampton, R. D.; Rupert, J. K.

2004-01-01

Many microgravity space-science experiments require vibratory acceleration levels that are unachievable without active isolation. The Boeing Corporation's active rack isolation system (ARIS) employs a novel combination of magnetic actuation and mechanical linkages to address these isolation requirements on the International Space Station. Effective model-based vibration isolation requires: (1) An isolation device, (2) an adequate dynamic; i.e., mathematical, model of that isolator, and (3) a suitable, corresponding controller. This Technical Memorandum documents the validation of that high-fidelity dynamic model of ARIS. The verification of this dynamics model was achieved by utilizing two commercial off-the-shelf (COTS) software tools: Deneb's ENVISION(registered trademark), and Online Dynamics Autolev(trademark). ENVISION is a robotics software package developed for the automotive industry that employs three-dimensional computer-aided design models to facilitate both forward and inverse kinematics analyses. Autolev is a DOS-based interpreter designed, in general, to solve vector-based mathematical problems and specifically to solve dynamics problems using Kane's method. The simplification of this model was achieved using the small-angle theorem for the joint angle of the ARIS actuators. This simplification has a profound effect on the overall complexity of the closed-form solution while yielding a closed-form solution easily employed using COTS control hardware.

User Interface Composition with COTS-UI and Trading Approaches: Application for Web-Based Environmental Information Systems

NASA Astrophysics Data System (ADS)

Criado, Javier; Padilla, Nicolás; Iribarne, Luis; Asensio, Jose-Andrés

Due to the globalization of the information and knowledge society on the Internet, modern Web-based Information Systems (WIS) must be flexible and prepared to be easily accessible and manageable in real-time. In recent times it has received a special interest the globalization of information through a common vocabulary (i.e., ontologies), and the standardized way in which information is retrieved on the Web (i.e., powerful search engines, and intelligent software agents). These same principles of globalization and standardization should also be valid for the user interfaces of the WIS, but they are built on traditional development paradigms. In this paper we present an approach to reduce the gap of globalization/standardization in the generation of WIS user interfaces by using a real-time "bottom-up" composition perspective with COTS-interface components (type interface widgets) and trading services.

Command Center Library Model Document. Comprehensive Approach to Reusable Defense Software (CARDS)

DTIC Science & Technology

1992-05-31

system, and functionality for specifying the layout of the document. 3.7.16.1 FrameMaker FrameMaker is a Commercial Off The Shelf (COTS) component...facilitating WYSIWYG creation of formatted reports with embedded graphics. FrameMaker is an advanced publishing tool that integrates word processing...available for the component FrameMaker : * Product evaluation reports in ASCII and postscript formats • Product assessment on line in model 0 Product

Exploration of COTS Ultrasonic NDE Methods for ISS MMOD Impact Analysis

NASA Technical Reports Server (NTRS)

Violette, Daniel P.; Koshti, Ajay; Stanley, David

2012-01-01

The high orbital speed of the International Space Station (ISS) has created a concern about Micro-Meteorite and Orbital Debris (MMOD). The possibility exists that such an impact could cause significant damage to the ISS pressure wall, and possibly lead to a pressure leak. This paper explores the potential of using commercial off-the-shelf (COTS) Ultrasonic Non-Destructive Evaluation (NDE) techniques in order to inspect and analyze MMOD impact damage if such an event would happen to occur. Different types of intra vehicular activity (IVA) Ultrasonic NDE equipment were evaluated, including the Olympus Omniscan MX and the General Electric Phasor XS. The equipment was tested by inspecting various aluminum standards and impact damage test plates in order to determine technological limitations of the equipment as well as the ease of use and availability of features. This study allowed for the design of scanning procedures in order to evaluate the extent of damage caused by an MMOD impact. Lastly, comparisons were drawn between the different pieces of COTS software and a recommendation is made based on each device s capability.

A Telemetry Browser Built with Java Components

NASA Astrophysics Data System (ADS)

Poupart, E.

In the context of CNES balloon scientific campaigns and telemetry survey field, a generic telemetry processing product, called TelemetryBrowser in the following, was developed reusing COTS, Java Components for most of them. Connection between those components relies on a software architecture based on parameter producers and parameter consumers. The first one transmit parameter values to the second one which has registered to it. All of those producers and consumers can be spread over the network thanks to Corba, and over every kind of workstation thanks to Java. This gives a very powerful mean to adapt to constraints like network bandwidth, or workstations processing or memory. It's also very useful to display and correlate at the same time information coming from multiple and various sources. An important point of this architecture is that the coupling between parameter producers and parameter consumers is reduced to the minimum and that transmission of information on the network is made asynchronously. So, if a parameter consumer goes down or runs slowly, there is no consequence on the other consumers, because producers don't wait for their consumers to finish their data processing before sending it to other consumers. An other interesting point is that parameter producers, also called TelemetryServers in the following are generated nearly automatically starting from a telemetry description using Flavori component. Keywords Java components, Corba, distributed application, OpenORBii, software reuse, COTS, Internet, Flavor. i Flavor (Formal Language for Audio-Visual Object Representation) is an object-oriented media representation language being developed at Columbia University. It is designed as an extension of Java and C++ and simplifies the development of applications that involve a significant media processing component (encoding, decoding, editing, manipulation, etc.) by providing bitstream representation semantics. (flavor.sourceforge.net) ii OpenORB provides a Java implementation of the OMG Corba 2.4.2 specification (openorb.sourceforge.net) 1/16

Developing an Empirical Account of a Community of Practice: Characterizing the Essential Tensions

ERIC Educational Resources Information Center

Barab, Sasha A.; Barnett, Michael; Squire, Kurt

2002-01-01

This article examines the potential of a learning-as-a-part-of-a-community approach, focusing on the participatory process of learning in a community-based, teacher education program; a Community of Teachers (CoT). CoT is a preparation program for preservice teachers working toward secondary teacher certification in which they join an on-going…

Meeting the Challenge of Distributed Real-Time & Embedded (DRE) Systems

DTIC Science & Technology

2012-05-10

IP RTOS Middleware Middleware Services DRE Applications Operating Sys & Protocols Hardware & Networks Middleware Middleware Services DRE...Services COTS & standards-based middleware, language, OS , network, & hardware platforms • Real-time CORBA (TAO) middleware • ADAPTIVE Communication...SPLs) F-15 product variant A/V 8-B product variant F/A 18 product variant UCAV product variant Software Produce-Line Hardware (CPU, Memory, I/O) OS

Aviation System Analysis Capability Executive Assistant Design

NASA Technical Reports Server (NTRS)

Roberts, Eileen; Villani, James A.; Osman, Mohammed; Godso, David; King, Brent; Ricciardi, Michael

1998-01-01

In this technical document, we describe the design developed for the Aviation System Analysis Capability (ASAC) Executive Assistant (EA) Proof of Concept (POC). We describe the genesis and role of the ASAC system, discuss the objectives of the ASAC system and provide an overview of components and models within the ASAC system, and describe the design process and the results of the ASAC EA POC system design. We also describe the evaluation process and results for applicable COTS software. The document has six chapters, a bibliography, three appendices and one attachment.

Aviation System Analysis Capability Executive Assistant Development

NASA Technical Reports Server (NTRS)

Roberts, Eileen; Villani, James A.; Anderson, Kevin; Book, Paul

1999-01-01

In this technical document, we describe the development of the Aviation System Analysis Capability (ASAC) Executive Assistant (EA) Proof of Concept (POC) and Beta version. We describe the genesis and role of the ASAC system, discuss the objectives of the ASAC system and provide an overview of components and models in the ASAC system, and describe the design process and the results of the ASAC EA POC and Beta system development. We also describe the evaluation process and results for applicable COTS software. The document has seven chapters, a bibliography, and two appendices.

Generic trending and analysis system

NASA Technical Reports Server (NTRS)

Keehan, Lori; Reese, Jay

1994-01-01

The Generic Trending and Analysis System (GTAS) is a generic spacecraft performance monitoring tool developed by NASA Code 511 and Loral Aerosys. It is designed to facilitate quick anomaly resolution and trend analysis. Traditionally, the job of off-line analysis has been performed using hardware and software systems developed for real-time spacecraft contacts; then, the systems were supplemented with a collection of tools developed by Flight Operations Team (FOT) members. Since the number of upcoming missions is increasing, NASA can no longer afford to operate in this manner. GTAS improves control center productivity and effectiveness because it provides a generic solution across multiple missions. Thus, GTAS eliminates the need for each individual mission to develop duplicate capabilities. It also allows for more sophisticated tools to be developed because it draws resources from several projects. In addition, the GTAS software system incorporates commercial off-the-shelf tools software (COTS) packages and reuses components of other NASA-developed systems wherever possible. GTAS has incorporated lessons learned from previous missions by involving the users early in the development process. GTAS users took a proactive role in requirements analysis, design, development, and testing. Because of user involvement, several special tools were designed and are now being developed. GTAS users expressed considerable interest in facilitating data collection for long term trending and analysis. As a result, GTAS provides easy access to large volumes of processed telemetry data directly in the control center. The GTAS archival and retrieval capabilities are supported by the integration of optical disk technology and a COTS relational database management system.

Overview of US Navy UAS Programs of Record to TTCP, MAD UAS Meeting

DTIC Science & Technology

2012-07-01

Development / integration / maintenance / sustainment of software code  Rapid integration of new capabilities across the Family of Systems...Operations & Support • Maintenance - GH • Basing - GH • Training - GH • Manpower - GH • COTS Hardware Communication • Ku band SATCOM • CDL • ARC...210 • Inmarsat • CAMA Operations & Support • Maintenance – MPRF/GH • Basing – MPRF/GH • Training – MPRF/GH • Manpower – MPRF/GH MOA

DAQ: Software Architecture for Data Acquisition in Sounding Rockets

NASA Technical Reports Server (NTRS)

Ahmad, Mohammad; Tran, Thanh; Nichols, Heidi; Bowles-Martinez, Jessica N.

2011-01-01

A multithreaded software application was developed by Jet Propulsion Lab (JPL) to collect a set of correlated imagery, Inertial Measurement Unit (IMU) and GPS data for a Wallops Flight Facility (WFF) sounding rocket flight. The data set will be used to advance Terrain Relative Navigation (TRN) technology algorithms being researched at JPL. This paper describes the software architecture and the tests used to meet the timing and data rate requirements for the software used to collect the dataset. Also discussed are the challenges of using commercial off the shelf (COTS) flight hardware and open source software. This includes multiple Camera Link (C-link) based cameras, a Pentium-M based computer, and Linux Fedora 11 operating system. Additionally, the paper talks about the history of the software architecture's usage in other JPL projects and its applicability for future missions, such as cubesats, UAVs, and research planes/balloons. Also talked about will be the human aspect of project especially JPL's Phaeton program and the results of the launch.

The Integration of COTS/GOTS within NASA's HST Command and Control System

NASA Technical Reports Server (NTRS)

Pfarr, Thomas; Reis, James E.

2001-01-01

NASA's mission critical Hubble Space Telescope (HST) command and control system has been re-engineered with commercial-off-the-shelf (COTS/GOTS) and minimal custom code. This paper focuses on the design of this new HST Control Center System (CCS) and the lessons learned throughout its development. CCS currently utilizes more than 30 COTS/GOTS products with an additional 1/2 million lines of custom glueware code; the new CCS exceeds the capabilities of the original system while significantly reducing the lines of custom code by more than 50%. The lifecycle of COTS/GOTS products will be examined including the package selection process, evaluation process, and integration process. The advantages, disadvantages, issues, concerns, and lessons learned for integrating COTS/GOTS into the NASA's mission critical HST CCS will be examined in detail. This paper will reveal the many hidden costs of COTS/GOTS solutions when compared to traditional custom code development efforts; this paper will show the high cost of COTS/GOTS solutions including training expenses, consulting fees, and long-term maintenance expenses.

Integration experiences and performance studies of A COTS parallel archive systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Hsing-bung; Scott, Cody; Grider, Bary

2010-01-01

Current and future Archive Storage Systems have been asked to (a) scale to very high bandwidths, (b) scale in metadata performance, (c) support policy-based hierarchical storage management capability, (d) scale in supporting changing needs of very large data sets, (e) support standard interface, and (f) utilize commercial-off-the-shelf(COTS) hardware. Parallel file systems have been asked to do the same thing but at one or more orders of magnitude faster in performance. Archive systems continue to move closer to file systems in their design due to the need for speed and bandwidth, especially metadata searching speeds such as more caching and lessmore » robust semantics. Currently the number of extreme highly scalable parallel archive solutions is very small especially those that will move a single large striped parallel disk file onto many tapes in parallel. We believe that a hybrid storage approach of using COTS components and innovative software technology can bring new capabilities into a production environment for the HPC community much faster than the approach of creating and maintaining a complete end-to-end unique parallel archive software solution. In this paper, we relay our experience of integrating a global parallel file system and a standard backup/archive product with a very small amount of additional code to provide a scalable, parallel archive. Our solution has a high degree of overlap with current parallel archive products including (a) doing parallel movement to/from tape for a single large parallel file, (b) hierarchical storage management, (c) ILM features, (d) high volume (non-single parallel file) archives for backup/archive/content management, and (e) leveraging all free file movement tools in Linux such as copy, move, ls, tar, etc. We have successfully applied our working COTS Parallel Archive System to the current world's first petaflop/s computing system, LANL's Roadrunner, and demonstrated its capability to address requirements of future archival storage systems.« less

Integration experiments and performance studies of a COTS parallel archive system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Hsing-bung; Scott, Cody; Grider, Gary

2010-06-16

Current and future Archive Storage Systems have been asked to (a) scale to very high bandwidths, (b) scale in metadata performance, (c) support policy-based hierarchical storage management capability, (d) scale in supporting changing needs of very large data sets, (e) support standard interface, and (f) utilize commercial-off-the-shelf (COTS) hardware. Parallel file systems have been asked to do the same thing but at one or more orders of magnitude faster in performance. Archive systems continue to move closer to file systems in their design due to the need for speed and bandwidth, especially metadata searching speeds such as more caching andmore » less robust semantics. Currently the number of extreme highly scalable parallel archive solutions is very small especially those that will move a single large striped parallel disk file onto many tapes in parallel. We believe that a hybrid storage approach of using COTS components and innovative software technology can bring new capabilities into a production environment for the HPC community much faster than the approach of creating and maintaining a complete end-to-end unique parallel archive software solution. In this paper, we relay our experience of integrating a global parallel file system and a standard backup/archive product with a very small amount of additional code to provide a scalable, parallel archive. Our solution has a high degree of overlap with current parallel archive products including (a) doing parallel movement to/from tape for a single large parallel file, (b) hierarchical storage management, (c) ILM features, (d) high volume (non-single parallel file) archives for backup/archive/content management, and (e) leveraging all free file movement tools in Linux such as copy, move, Is, tar, etc. We have successfully applied our working COTS Parallel Archive System to the current world's first petafiop/s computing system, LANL's Roadrunner machine, and demonstrated its capability to address requirements of future archival storage systems.« less

Safe to Fly: Certifying COTS Hardware for Spaceflight

NASA Technical Reports Server (NTRS)

Fichuk, Jessica L.

2011-01-01

Providing hardware for the astronauts to use on board the Space Shuttle or International Space Station (ISS) involves a certification process that entails evaluating hardware safety, weighing risks, providing mitigation, and verifying requirements. Upon completion of this certification process, the hardware is deemed safe to fly. This process from start to finish can be completed as quickly as 1 week or can take several years in length depending on the complexity of the hardware and whether the item is a unique custom design. One area of cost and schedule savings that NASA implements is buying Commercial Off the Shelf (COTS) hardware and certifying it for human spaceflight as safe to fly. By utilizing commercial hardware, NASA saves time not having to develop, design and build the hardware from scratch, as well as a timesaving in the certification process. By utilizing COTS hardware, the current detailed certification process can be simplified which results in schedule savings. Cost savings is another important benefit of flying COTS hardware. Procuring COTS hardware for space use can be more economical than custom building the hardware. This paper will investigate the cost savings associated with certifying COTS hardware to NASA s standards rather than performing a custom build.

SMC Message Browser Projects

NASA Technical Reports Server (NTRS)

Wichmann, Benjamin C.

2013-01-01

I work directly with the System Monitoring and Control (SMC) software engineers who develop, test and release custom and commercial software in support of the Kennedy Space Center Spaceport Command and Control System. (SCCS). SMC uses Commercial Off-The-Shelf (COTS) Enterprise Management Systems (EMS) software which provides a centralized subsystem for configuring, monitoring, and controlling SCCS hardware and software used in the Control Rooms. There are multiple projects being worked on using the COTS EMS software. I am currently working with the HP Operations Manager for UNIX (OMU) software which allows Master Console Operators (MCO) to access, view and interpret messages regarding the status of the SCCS hardware and software. The OMU message browser gets cluttered with messages which can make it difficult for the MCO to manage. My main project involves determining ways to reduce the number of messages being displayed in the OMU message browser. I plan to accomplish this task in two different ways: (1) by correlating multiple messages into one single message being displayed and (2) to create policies that will determine the significance of each message and whether or not it needs to be displayed to the MCO. The core idea is to lessen the number of messages being sent to the OMU message browser so the MCO can more effectively use it.

Proceedings of the Twenty-Fourth Annual Software Engineering Workshop

NASA Technical Reports Server (NTRS)

2000-01-01

On December 1 and 2, the Software Engineering Laboratory (SEL), a consortium composed of NASA/Goddard, the University of Maryland, and CSC, held the 24th Software Engineering Workshop (SEW), the last of the millennium. Approximately 240 people attended the 2-day workshop. Day 1 was composed of four sessions: International Influence of the Software Engineering Laboratory; Object Oriented Testing and Reading; Software Process Improvement; and Space Software. For the first session, three internationally known software process experts discussed the influence of the SEL with respect to software engineering research. In the Space Software session, prominent representatives from three different NASA sites- GSFC's Marti Szczur, the Jet Propulsion Laboratory's Rick Doyle, and the Ames Research Center IV&V Facility's Lou Blazy- discussed the future of space software in their respective centers. At the end of the first day, the SEW sponsored a reception at the GSFC Visitors' Center. Day 2 also provided four sessions: Using the Experience Factory; A panel discussion entitled "Software Past, Present, and Future: Views from Government, Industry, and Academia"; Inspections; and COTS. The day started with an excellent talk by CSC's Frank McGarry on "Attaining Level 5 in CMM Process Maturity." Session 2, the panel discussion on software, featured NASA Chief Information Officer Lee Holcomb (Government), our own Jerry Page (Industry), and Mike Evangelist of the National Science Foundation (Academia). Each presented his perspective on the most important developments in software in the past 10 years, in the present, and in the future.

REE radiation fault model: a tool for organizing and communication radiation test data and construction COTS based spacebourne computing systems

NASA Technical Reports Server (NTRS)

Ferraro, R.; Some, R.

2002-01-01

The growth in data rates of instruments on future NASA spacecraft continues to outstrip the improvement in communications bandwidth and processing capabilities of radiation-hardened computers. Sophisticated autonomous operations strategies will further increase the processing workload. Given the reductions in spacecraft size and available power, standard radiation hardened computing systems alone will not be able to address the requirements of future missions. The REE project was intended to overcome this obstacle by developing a COTS- based supercomputer suitable for use as a science and autonomy data processor in most space environments. This development required a detailed knowledge of system behavior in the presence of Single Event Effect (SEE) induced faults so that mitigation strategies could be designed to recover system level reliability while maintaining the COTS throughput advantage. The REE project has developed a suite of tools and a methodology for predicting SEU induced transient fault rates in a range of natural space environments from ground-based radiation testing of component parts. In this paper we provide an overview of this methodology and tool set with a concentration on the radiation fault model and its use in the REE system development methodology. Using test data reported elsewhere in this and other conferences, we predict upset rates for a particular COTS single board computer configuration in several space environments.

Web-Based Environment for Maintaining Legacy Software

NASA Technical Reports Server (NTRS)

Tigges, Michael; Thompson, Nelson; Orr, Mark; Fox, Richard

2007-01-01

Advanced Tool Integration Environment (ATIE) is the name of both a software system and a Web-based environment created by the system for maintaining an archive of legacy software and expertise involved in developing the legacy software. ATIE can also be used in modifying legacy software and developing new software. The information that can be encapsulated in ATIE includes experts documentation, input and output data of tests cases, source code, and compilation scripts. All of this information is available within a common environment and retained in a database for ease of access and recovery by use of powerful search engines. ATIE also accommodates the embedment of supporting software that users require for their work, and even enables access to supporting commercial-off-the-shelf (COTS) software within the flow of the experts work. The flow of work can be captured by saving the sequence of computer programs that the expert uses. A user gains access to ATIE via a Web browser. A modern Web-based graphical user interface promotes efficiency in the retrieval, execution, and modification of legacy code. Thus, ATIE saves time and money in the support of new and pre-existing programs.

XML: James Webb Space Telescope Database Issues, Lessons, and Status

NASA Technical Reports Server (NTRS)

Detter, Ryan; Mooney, Michael; Fatig, Curtis

2003-01-01

This paper will present the current concept using extensible Markup Language (XML) as the underlying structure for the James Webb Space Telescope (JWST) database. The purpose of using XML is to provide a JWST database, independent of any portion of the ground system, yet still compatible with the various systems using a variety of different structures. The testing of the JWST Flight Software (FSW) started in 2002, yet the launch is scheduled for 2011 with a planned 5-year mission and a 5-year follow on option. The initial database and ground system elements, including the commands, telemetry, and ground system tools will be used for 19 years, plus post mission activities. During the Integration and Test (I&T) phases of the JWST development, 24 distinct laboratories, each geographically dispersed, will have local database tools with an XML database. Each of these laboratories database tools will be used for the exporting and importing of data both locally and to a central database system, inputting data to the database certification process, and providing various reports. A centralized certified database repository will be maintained by the Space Telescope Science Institute (STScI), in Baltimore, Maryland, USA. One of the challenges for the database is to be flexible enough to allow for the upgrade, addition or changing of individual items without effecting the entire ground system. Also, using XML should allow for the altering of the import and export formats needed by the various elements, tracking the verification/validation of each database item, allow many organizations to provide database inputs, and the merging of the many existing database processes into one central database structure throughout the JWST program. Many National Aeronautics and Space Administration (NASA) projects have attempted to take advantage of open source and commercial technology. Often this causes a greater reliance on the use of Commercial-Off-The-Shelf (COTS), which is often limiting. In our review of the database requirements and the COTS software available, only very expensive COTS software will meet 90% of requirements. Even with the high projected initial cost of COTS, the development and support for custom code over the 19-year mission period was forecasted to be higher than the total licensing costs. A group did look at reusing existing database tools and formats. If the JWST database was already in a mature state, the reuse made sense, but with the database still needing to handing the addition of different types of command and telemetry structures, defining new spacecraft systems, accept input and export to systems which has not been defined yet, XML provided the flexibility desired. It remains to be determined whether the XML database will reduce the over all cost for the JWST mission.

Technical Data Interoperability (TDI) Pathfinder Via Emerging Standards

NASA Technical Reports Server (NTRS)

Conroy, Mike; Gill, Paul; Hill, Bradley; Ibach, Brandon; Jones, Corey; Ungar, David; Barch, Jeffrey; Ingalls, John; Jacoby, Joseph; Manning, Josh;

Digital video timing analyzer for the evaluation of PC-based real-time simulation systems

NASA Astrophysics Data System (ADS)

Jones, Shawn R.; Crosby, Jay L.; Terry, John E., Jr.

2009-05-01

Due to the rapid acceleration in technology and the drop in costs, the use of commercial off-the-shelf (COTS) PC-based hardware and software components for digital and hardware-in-the-loop (HWIL) simulations has increased. However, the increase in PC-based components creates new challenges for HWIL test facilities such as cost-effective hardware and software selection, system configuration and integration, performance testing, and simulation verification/validation. This paper will discuss how the Digital Video Timing Analyzer (DiViTA) installed in the Aviation and Missile Research, Development and Engineering Center (AMRDEC) provides quantitative characterization data for PC-based real-time scene generation systems. An overview of the DiViTA is provided followed by details on measurement techniques, applications, and real-world examples of system benefits.

COTS technologies for telemedicine applications.

PubMed

Triunfo, Riccardo; Tumbarello, Roberto; Sulis, Alessandro; Zanetti, Gianluigi; Lianas, Luca; Meloni, Vittorio; Frexia, Francesca

2010-01-01

To demonstrate a simple low-cost system for tele-echocardiology, focused on paediatric cardiology applications. The system was realized using open-source software and COTS technologies. It is based on the transmission of two simultaneous video streams, obtained by direct digitization of the output of an ultrasound machine and by a netcam showing the examination that is taking place. These streams are then embedded into a web page so they are accessible, together with basic video controls, via a standard web browser. The system can also record video streams on a server for further use. The system was tested on a small group of neonatal cases with suspected cardiopathies for a preliminary assessment of its features and diagnostic capabilities. Both the clinical and technological results were encouraging and are leading the way for further experimentation. The presented system can transfer clinical images and videos in an efficient way and in real time. It can be used in the same hospital to support internal consultancy requests, in remote areas using Internet connections and for didactic purposes using low cost COTS appliances and simple interfaces for end users. The solution proposed can be extended to control different medical appliances in those remote hospitals.

COX2 expression and Erk1/Erk2 activity mediate Cot-induced cell migration.

PubMed

Rodríguez, Cristina; López, Pilar; Pozo, Maite; Duce, Antonio Martín; López-Pelaéz, Marta; Fernández, Margarita; Alemany, Susana

2008-09-01

The MAPKKK8 Cot/tpl-2, identified as an oncogene (Cot-T), participates in the intracellular signaling activated by members of the TLR and TNFalpha receptor superfamilies. Here we demonstrate that Cot promotes cell migration by regulating different steps involved in this process, such as cell adhesion and metalloproteinase activity. Indeed, Cot also regulates the cytoskeleton and Cot-T overexpression provokes the polarization of microtubules and the loss of stress fibers. Moreover, and in accordance with the increased Rac-GTP levels observed, Cot-T overexpressing cells develop more lamellipodia than control cells. Conversely, depletion of endogenous Cot increases the formation of stress fibers which is correlated with the high levels of Rho-GTP observed in these cells. In addition, the increase in COX2 expression and the activation of Erk1/2 regulated by Cot are essential for the induction of cell migration. Together, these data provide evidence of a new role for both proto-oncogenic and oncogenic Cot.

Implementation of Phased Array Antenna Technology Providing a Wireless Local Area Network to Enhance Port Security and Maritime Interdiction Operations

DTIC Science & Technology

2009-09-01

boarding team, COTS, WLAN, smart antenna, OpenVPN application, wireless base station, OFDM, latency, point-to-point wireless link. 16. PRICE CODE 17...16 c. SSL/TLS .................................17 2. OpenVPN ......................................17 III. EXPERIMENT METHODOLOGY...network frame at Layer 2 has already been secured by encryption at a higher level. 2. OpenVPN OpenVPN is open source software that provides a VPN

75 FR 25161 - Defense Federal Acquisition Regulation Supplement; Presumption of Development at Private Expense

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-07

... asserted restrictions on technical data and computer software. DATES: Comments on the proposed rule should... restrictions on technical data and computer software. More specifically, the proposed rule affects these...) items (as defined at 41 U.S.C. 431(c)). Since COTS items are a subtype of commercial items, this change...

RelB-induced expression of Cot, an MAP3K family member, rescues RANKL-induced osteoclastogenesis in alymphoplasia mice by promoting NF-κB2 processing by IKKα.

PubMed

Taniguchi, Rei; Fukushima, Hidefumi; Osawa, Kenji; Maruyama, Toshimasa; Yasuda, Hisataka; Weih, Falk; Doi, Takahiro; Maki, Kenshi; Jimi, Eijiro

2014-03-14

The alternative nuclear factor-κB (NF-κB) pathway, mainly the RelB-p52 heterodimer, plays important roles in bone metabolism through an unknown mechanism. We have previously reported that alymphoplasia (aly/aly) mice, which lack active NF-κB-inducing kinase (NIK), show mild osteopetrosis due to the inhibition of osteoclastogenesis. p100 retains RelB in the cytoplasm and inhibits RANKL-induced osteoclastogenesis in aly/aly cells. Furthermore, the overexpression of RelB in aly/aly cells rescues RANKL-induced osteoclastogenesis by inducing p100 processing. In contrast, the overexpression of p65 in aly/aly cells has no effect. However, the overexpression of RelB fails to rescue RANKL-induced osteoclastogenesis in the presence of p100ΔGRR, which cannot be processed to p52, suggesting that p100 processing is a key step in RelB-rescued, RANKL-induced osteoclastogenesis in aly/aly cells. In this study, Cot (cancer Osaka thyroid), an MAP3K, was up-regulated by RelB overexpression. Analysis of the Cot promoter demonstrated that p65 and RelB bound to the distal NF-κB-binding site and that RelB but not p65 bound to the proximal NF-κB-binding site in the Cot promoter. The knocking down of Cot expression significantly reduced the RANKL-induced osteoclastogenesis induced by RelB overexpression. The phosphorylation of IKKα at threonine 23 and its kinase activity were indispensable for the processing of p100 and osteoclastogenesis by RelB-induced Cot. Finally, constitutively activated Akt enhanced osteoclastogenesis by RelB-induced Cot, and a dominant-negative form of Akt significantly inhibited it. Taken together, these results indicate that the overexpression of RelB restores RANKL-induced osteoclastogenesis by activation of Akt/Cot/IKKα-induced p100 processing.

RelB-induced Expression of Cot, an MAP3K Family Member, Rescues RANKL-induced Osteoclastogenesis in Alymphoplasia Mice by Promoting NF-κB2 Processing by IKKα*

PubMed Central

Taniguchi, Rei; Fukushima, Hidefumi; Osawa, Kenji; Maruyama, Toshimasa; Yasuda, Hisataka; Weih, Falk; Doi, Takahiro; Maki, Kenshi; Jimi, Eijiro

2014-01-01

The alternative nuclear factor-κB (NF-κB) pathway, mainly the RelB-p52 heterodimer, plays important roles in bone metabolism through an unknown mechanism. We have previously reported that alymphoplasia (aly/aly) mice, which lack active NF-κB-inducing kinase (NIK), show mild osteopetrosis due to the inhibition of osteoclastogenesis. p100 retains RelB in the cytoplasm and inhibits RANKL-induced osteoclastogenesis in aly/aly cells. Furthermore, the overexpression of RelB in aly/aly cells rescues RANKL-induced osteoclastogenesis by inducing p100 processing. In contrast, the overexpression of p65 in aly/aly cells has no effect. However, the overexpression of RelB fails to rescue RANKL-induced osteoclastogenesis in the presence of p100ΔGRR, which cannot be processed to p52, suggesting that p100 processing is a key step in RelB-rescued, RANKL-induced osteoclastogenesis in aly/aly cells. In this study, Cot (cancer Osaka thyroid), an MAP3K, was up-regulated by RelB overexpression. Analysis of the Cot promoter demonstrated that p65 and RelB bound to the distal NF-κB-binding site and that RelB but not p65 bound to the proximal NF-κB-binding site in the Cot promoter. The knocking down of Cot expression significantly reduced the RANKL-induced osteoclastogenesis induced by RelB overexpression. The phosphorylation of IKKα at threonine 23 and its kinase activity were indispensable for the processing of p100 and osteoclastogenesis by RelB-induced Cot. Finally, constitutively activated Akt enhanced osteoclastogenesis by RelB-induced Cot, and a dominant-negative form of Akt significantly inhibited it. Taken together, these results indicate that the overexpression of RelB restores RANKL-induced osteoclastogenesis by activation of Akt/Cot/IKKα-induced p100 processing. PMID:24488495

Software Tools to Support the Assessment of System Health

NASA Technical Reports Server (NTRS)

Melcher, Kevin J.

2013-01-01

This presentation provides an overview of three software tools that were developed by the NASA Glenn Research Center to support the assessment of system health: the Propulsion Diagnostic Method Evaluation Strategy (ProDIMES), the Systematic Sensor Selection Strategy (S4), and the Extended Testability Analysis (ETA) tool. Originally developed to support specific NASA projects in aeronautics and space, these software tools are currently available to U.S. citizens through the NASA Glenn Software Catalog. The ProDiMES software tool was developed to support a uniform comparison of propulsion gas path diagnostic methods. Methods published in the open literature are typically applied to dissimilar platforms with different levels of complexity. They often address different diagnostic problems and use inconsistent metrics for evaluating performance. As a result, it is difficult to perform a one ]to ]one comparison of the various diagnostic methods. ProDIMES solves this problem by serving as a theme problem to aid in propulsion gas path diagnostic technology development and evaluation. The overall goal is to provide a tool that will serve as an industry standard, and will truly facilitate the development and evaluation of significant Engine Health Management (EHM) capabilities. ProDiMES has been developed under a collaborative project of The Technical Cooperation Program (TTCP) based on feedback provided by individuals within the aircraft engine health management community. The S4 software tool provides a framework that supports the optimal selection of sensors for health management assessments. S4 is structured to accommodate user ]defined applications, diagnostic systems, search techniques, and system requirements/constraints. One or more sensor suites that maximize this performance while meeting other user ]defined system requirements that are presumed to exist. S4 provides a systematic approach for evaluating combinations of sensors to determine the set or sets of sensors that optimally meet the performance goals and the constraints. It identifies optimal sensor suite solutions by utilizing a merit (i.e., cost) function with one of several available optimization approaches. As part of its analysis, S4 can expose fault conditions that are difficult to diagnose due to an incomplete diagnostic philosophy and/or a lack of sensors. S4 was originally developed and applied to liquid rocket engines. It was subsequently used to study the optimized selection of sensors for a simulation ]based aircraft engine diagnostic system. The ETA Tool is a software ]based analysis tool that augments the testability analysis and reporting capabilities of a commercial ]off ]the ]shelf (COTS) package. An initial diagnostic assessment is performed by the COTS software using a user ]developed, qualitative, directed ]graph model of the system being analyzed. The ETA Tool accesses system design information captured within the model and the associated testability analysis output to create a series of six reports for various system engineering needs. These reports are highlighted in the presentation. The ETA Tool was developed by NASA to support the verification of fault management requirements early in the Launch Vehicle process. Due to their early development during the design process, the TEAMS ]based diagnostic model and the ETA Tool were able to positively influence the system design by highlighting gaps in failure detection, fault isolation, and failure recovery.

Low Resolution Picture Transmission (LRPT) Demonstration System

NASA Technical Reports Server (NTRS)

Fong, Wai; Yeh, Pen-Shu; Sank, Victor; Nyugen, Xuan; Xia, Wei; Duran, Steve; Day, John H. (Technical Monitor)

2002-01-01

Low-Resolution Picture Transmission (LRPT) is a proposed standard for direct broadcast transmission of satellite weather images. This standard is a joint effort by the European Organization for the Exploitation of Meteorological Satellites (EUMETSAT) and the National Oceanic Atmospheric Administration (NOAA). As a digital transmission scheme, its purpose is to replace the current analog Automatic Picture Transmission (APT) system for use in the Meteorological Operational (METOP) satellites. Goddard Space Flight Center has been tasked to build an LRPT Demonstration System (LDS). It's main objective is to develop or demonstrate the feasibility of a low-cost receiver utilizing a Personal Computer (PC) as the primary processing component and determine the performance of the protocol in the simulated Radio Frequency (RF) environment. The approach would consist of two phases. In the phase 1, a Commercial-off-the-Shelf (COTS) Modulator-Demodulator (MODEM) board that would perform RF demodulation would be purchased allowing the Central Processing Unit (CPU) to perform the Consultative Committee for Space Data Systems (CCSDS) protocol processing. Also since the weather images are compressed the PC would perform the decompression. Phase 1 was successfully demonstrated on December 1997. Phase 2 consists of developing a high-fidelity receiver, transmitter and environment simulator. Its goal is to find out how the METOP Specification performs in a simulated noise environment in a cost-effective receiver. The approach would be to produce a receiver using as much software as possible to perform front-end processing to take advantage of the latest high-speed PCs. Thus the COTS MODEM used in Phase 1 is performing RF demodulation along with data acquisition providing data to the receiving software. Also, environment simulator is produced using the noise patterns generated by Institute for Telecommunications Sciences (ITS) from their noise environment study.

COTS-Based Fault Tolerance in Deep Space: Qualitative and Quantitative Analyses of a Bus Network Architecture

NASA Technical Reports Server (NTRS)

Tai, Ann T.; Chau, Savio N.; Alkalai, Leon

2000-01-01

Using COTS products, standards and intellectual properties (IPs) for all the system and component interfaces is a crucial step toward significant reduction of both system cost and development cost as the COTS interfaces enable other COTS products and IPs to be readily accommodated by the target system architecture. With respect to the long-term survivable systems for deep-space missions, the major challenge for us is, under stringent power and mass constraints, to achieve ultra-high reliability of the system comprising COTS products and standards that are not developed for mission-critical applications. The spirit of our solution is to exploit the pertinent standard features of a COTS product to circumvent its shortcomings, though these standard features may not be originally designed for highly reliable systems. In this paper, we discuss our experiences and findings on the design of an IEEE 1394 compliant fault-tolerant COTS-based bus architecture. We first derive and qualitatively analyze a -'stacktree topology" that not only complies with IEEE 1394 but also enables the implementation of a fault-tolerant bus architecture without node redundancy. We then present a quantitative evaluation that demonstrates significant reliability improvement from the COTS-based fault tolerance.

Taking advantage of ground data systems attributes to achieve quality results in testing software

NASA Technical Reports Server (NTRS)

Sigman, Clayton B.; Koslosky, John T.; Hageman, Barbara H.

1994-01-01

During the software development life cycle process, basic testing starts with the development team. At the end of the development process, an acceptance test is performed for the user to ensure that the deliverable is acceptable. Ideally, the delivery is an operational product with zero defects. However, the goal of zero defects is normally not achieved but is successful to various degrees. With the emphasis on building low cost ground support systems while maintaining a quality product, a key element in the test process is simulator capability. This paper reviews the Transportable Payload Operations Control Center (TPOCC) Advanced Spacecraft Simulator (TASS) test tool that is used in the acceptance test process for unmanned satellite operations control centers. The TASS is designed to support the development, test and operational environments of the Goddard Space Flight Center (GSFC) operations control centers. The TASS uses the same basic architecture as the operations control center. This architecture is characterized by its use of distributed processing, industry standards, commercial off-the-shelf (COTS) hardware and software components, and reusable software. The TASS uses much of the same TPOCC architecture and reusable software that the operations control center developer uses. The TASS also makes use of reusable simulator software in the mission specific versions of the TASS. Very little new software needs to be developed, mainly mission specific telemetry communication and command processing software. By taking advantage of the ground data system attributes, successful software reuse for operational systems provides the opportunity to extend the reuse concept into the test area. Consistency in test approach is a major step in achieving quality results.

A COTS-Based Replacement Strategy for Aging Avionics Computers

DTIC Science & Technology

2001-12-01

Communication Control Unit. A COTS-Based Replacement Strategy for Aging Avionics Computers COTS Microprocessor Real Time Operating System New Native Code...Native Code Objec ts Native Code Thread Real - Time Operating System Legacy Function x Virtual Component Environment Context Switch Thunk Add-in Replace

Real-Time Data Display

NASA Technical Reports Server (NTRS)

Pedings, Marc

2007-01-01

RT-Display is a MATLAB-based data acquisition environment designed to use a variety of commercial off-the-shelf (COTS) hardware to digitize analog signals to a standard data format usable by other post-acquisition data analysis tools. This software presents the acquired data in real time using a variety of signal-processing algorithms. The acquired data is stored in a standard Operator Interactive Signal Processing Software (OISPS) data-formatted file. RT-Display is primarily configured to use the Agilent VXI (or equivalent) data acquisition boards used in such systems as MIDDAS (Multi-channel Integrated Dynamic Data Acquisition System). The software is generalized and deployable in almost any testing environment, without limitations or proprietary configuration for a specific test program or project. With the Agilent hardware configured and in place, users can start the program and, in one step, immediately begin digitizing multiple channels of data. Once the acquisition is completed, data is converted into a common binary format that also can be translated to specific formats used by external analysis software, such as OISPS and PC-Signal (product of AI Signal Research Inc.). RT-Display at the time of this reporting was certified on Agilent hardware capable of acquisition up to 196,608 samples per second. Data signals are presented to the user on-screen simultaneously for 16 channels. Each channel can be viewed individually, with a maximum capability of 160 signal channels (depending on hardware configuration). Current signal presentations include: time data, fast Fourier transforms (FFT), and power spectral density plots (PSD). Additional processing algorithms can be easily incorporated into this environment.

Addressing Software Security

NASA Technical Reports Server (NTRS)

Bailey, Brandon

2015-01-01

Historically security within organizations was thought of as an IT function (web sites/servers, email, workstation patching, etc.) Threat landscape has evolved (Script Kiddies, Hackers, Advanced Persistent Threat (APT), Nation States, etc.) Attack surface has expanded -Networks interconnected!! Some security posture factors Network Layer (Routers, Firewalls, etc.) Computer Network Defense (IPS/IDS, Sensors, Continuous Monitoring, etc.) Industrial Control Systems (ICS) Software Security (COTS, FOSS, Custom, etc.)

Feature Selection for Evolutionary Commercial-off-the-Shelf Software: Studies Focusing on Time-to-Market, Innovation and Hedonic-Utilitarian Trade-Offs

ERIC Educational Resources Information Center

Kakar, Adarsh Kumar

2013-01-01

Feature selection is one of the most important decisions made by product managers. This three article study investigates the concepts, tools and techniques for making trade-off decisions of introducing new features in evolving Commercial-Off-The-Shelf (COTS) software products. The first article investigates the efficacy of various feature…

Application of a cell-once-through perfusion strategy for production of recombinant antibody from rCHO cells in a Centritech Lab II centrifuge system.

PubMed

Kim, Byoung Jin; Chang, Ho Nam; Oh, Duk Jae

2007-01-01

Based upon the results of scale-down intermittent perfusion processes, a cell-once-through (COT) perfusion concept was applied to a dual bioreactor system coupled to a Centritech Lab II centrifuge for culture of recombinant Chinese hamster ovary (rCHO) cells for monoclonal antibody production. In this new culture mode, i.e., the COT perfusion process, total spent medium was transferred to the centrifuge and a fixed percentage was removed. Approximately 99% of the viable cells are transferred to another bioreactor filled with fresh medium by single operation of the Centritech Lab II centrifuge system for about 30 min. Accordingly, a significant reduction of the cell-passage frequency to the centrifuge led to minimization of cell damage caused by mechanical shear stress, oxygen limitation, nutrient limitation, and low temperature outside the bioreactor. The effects of culture temperature shift and fortified medium on cell growth and recombinant antibody production in the COT perfusion process were investigated. Although the suppressive effects of low culture temperature on cell growth led to a loss of stability in a long-term COT perfusion culture system, the average antibody concentration at 33 degrees C was 157.8 mg/L, approximately 2.4-fold higher than that at 37 degrees C. By the use of a fortified medium at 37 degrees C, rCHO cells were maintained at high density above 1.2 x 10(7) cells/mL, and antibody was produced continuously in a range of 260-280 mg/L in a stable long-term COT perfusion culture. The proposed new culture mode, the COT perfusion approach, guarantees the recovery of rCHO cells damaged by lowered temperature or high lactate and ammonium concentration. It will be an attractive choice for minimization of cell damage and stable long-term antibody production with high cell density.

The design of a petabyte archive and distribution system for the NASA ECS project

NASA Technical Reports Server (NTRS)

Caulk, Parris M.

1994-01-01

The NASA EOS Data and Information System (EOSDIS) Core System (ECS) will contain one of the largest data management systems ever built - the ECS Science and Data Processing System (SDPS). SDPS is designed to support long term Global Change Research by acquiring, producing, and storing earth science data, and by providing efficient means for accessing and manipulating that data. The first two releases of SDPS, Release A and Release B, will be operational in 1997 and 1998, respectively. Release B will be deployed at eight Distributed Active Archiving Centers (DAAC's). Individual DAAC's will archive different collections of earth science data, and will vary in archive capacity. The storage and management of these data collections is the responsibility of the SDPS Data Server subsystem. It is anticipated that by the year 2001, the Data Server subsystem at the Goddard DAAC must support a near-line data storage capacity of one petabyte. The development of SDPS is a system integration effort in which COTS products will be used in favor of custom components in very possible way. Some software and hardware capabilities required to meet ECS data volume and storage management requirements beyond 1999 are not yet supported by available COTS products. The ECS project will not undertake major custom development efforts to provide these capabilities. Instead, SDPS and its Data Server subsystem are designed to support initial implementations with current products, and provide an evolutionary framework that facilitates the introduction of advanced COTS products as they become available. This paper provides a high-level description of the Data Server subsystem design from a COTS integration standpoint, and discussed some of the major issues driving the design. The paper focuses on features of the design that will make the system scalable and adaptable to changing technologies.

Air Force and the Cyberspace Mission: Defending the Air Force’s Computer Network in the Future

DTIC Science & Technology

2007-12-01

computers, their operating systems and software purchased by the Air Force are commercial off-the-shelf (COTS) components, often manufactured abroad due...crystal clear 2003 information security report: “The U.S. Department of Defense (DOD) relies too much on commercial software , doesn’t know who is...creating the software , and faces other significant cybersecurity problems.”11 This paper explores the topic of defense of the cyberspace domain by

Automation Hooks Architecture Trade Study for Flexible Test Orchestration

NASA Technical Reports Server (NTRS)

Lansdowne, Chatwin A.; Maclean, John R.; Graffagnino, Frank J.; McCartney, Patrick A.

2010-01-01

We describe the conclusions of a technology and communities survey supported by concurrent and follow-on proof-of-concept prototyping to evaluate feasibility of defining a durable, versatile, reliable, visible software interface to support strategic modularization of test software development. The objective is that test sets and support software with diverse origins, ages, and abilities can be reliably integrated into test configurations that assemble and tear down and reassemble with scalable complexity in order to conduct both parametric tests and monitored trial runs. The resulting approach is based on integration of three recognized technologies that are currently gaining acceptance within the test industry and when combined provide a simple, open and scalable test orchestration architecture that addresses the objectives of the Automation Hooks task. The technologies are automated discovery using multicast DNS Zero Configuration Networking (zeroconf), commanding and data retrieval using resource-oriented Restful Web Services, and XML data transfer formats based on Automatic Test Markup Language (ATML). This open-source standards-based approach provides direct integration with existing commercial off-the-shelf (COTS) analysis software tools.

Secure Video Surveillance System Acquisition Software

DOE Office of Scientific and Technical Information (OSTI.GOV)

2009-12-04

The SVSS Acquisition Software collects and displays video images from two cameras through a VPN, and store the images onto a collection controller. The software is configured to allow a user to enter a time window to display up to 2 1/2, hours of video review. The software collects images from the cameras at a rate of 1 image per second and automatically deletes images older than 3 hours. The software code operates in a linux environment and can be run in a virtual machine on Windows XP. The Sandia software integrates the different COTS software together to build themore » video review system.« less

Unfolding pathway of CotA-laccase and the role of copper on the prevention of refolding through aggregation of the unfolded state

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fernandes, Andre T.; Lopes, Carlos; Martins, Ligia O.

2012-06-08

Highlights: Black-Right-Pointing-Pointer CotA-laccase unfolds with an intermediate state. Black-Right-Pointing-Pointer Copper stabilizes the native and the intermediate state. Black-Right-Pointing-Pointer Copper binding to the unfolded state prevents refolding through protein aggregation. Black-Right-Pointing-Pointer Copper incorporation in CotA-laccase occurs as a later step during folding. -- Abstract: Copper is a redox-active metal and the main player in electron transfer reactions occurring in multicopper oxidases. The role of copper in the unfolding pathway and refolding of the multicopper oxidase CotA laccase in vitro was solved using double-jump stopped-flow experiments. Unfolding of apo- and holo-CotA was described as a three-state process with accumulation of an intermediatemore » in between the native and unfolded state. Copper stabilizes the native holo-CotA but also the intermediate state showing that copper is still bound to this state. Also, copper binds to unfolded holo-CotA in a non-native coordination promoting CotA aggregation and preventing refolding to the native structure. These results gather information on unfolding/folding pathways of multicopper oxidases and show that copper incorporation in vivo should be a tight controlled process as copper binding to the unfolded state under native conditions promotes protein aggregation.« less

Customizing graphical user interface technology for spacecraft control centers

NASA Technical Reports Server (NTRS)

Beach, Edward; Giancola, Peter; Gibson, Steven; Mahmot, Ronald

1993-01-01

The Transportable Payload Operations Control Center (TPOCC) project is applying the latest in graphical user interface technology to the spacecraft control center environment. This project of the Mission Operations Division's (MOD) Control Center Systems Branch (CCSB) at NASA Goddard Space Flight Center (GSFC) has developed an architecture for control centers which makes use of a distributed processing approach and the latest in Unix workstation technology. The TPOCC project is committed to following industry standards and using commercial off-the-shelf (COTS) hardware and software components wherever possible to reduce development costs and to improve operational support. TPOCC's most successful use of commercial software products and standards has been in the development of its graphical user interface. This paper describes TPOCC's successful use and customization of four separate layers of commercial software products to create a flexible and powerful user interface that is uniquely suited to spacecraft monitoring and control.

Prototyping a 10 Gigabit-Ethernet Event-Builder for the CTA Camera Server

NASA Astrophysics Data System (ADS)

Hoffmann, Dirk; Houles, Julien

2012-12-01

While the Cherenkov Telescope Array will end its Preperatory Phase in 2012 or 2013 with the publication of a Technical Design Report, our lab has undertaken within the french CTA community the design and prototyping of a Camera-Server, which is a PC architecture based computer, used as a switchboard assigned to each of a hundred telescopes to handle a maximum amount of scientific data recorded by each telescope. Our work aims for a data acquisition hardware and software system for the scientific raw data at optimal speed. We have evaluated the maximum performance that can be obtained by choosing standard (COTS) hardware and software (Linux) in conjunction with a 10 Gb/s switch.

Development and Implementation of the DHAPP Military eHealth Information Network System.

PubMed

Kratz, Mary; Thomas, Anne; Hora, Ricardo; Vera, Delphis; Lutz, Mickey; Johnson, Mark D

2017-01-01

As the Joint United Nations Programme on HIV/AIDS, the Global Fund, and the US President's Emergency Plan for AIDS Relief focus on reaching 90-90-90 goals, military health systems are scaling up to meet the data demands of these ambitious objectives. Since 2008, the US Department of Defense HIV/AIDS Prevention Program (DHAPP) has been working with military partners in 14 countries on implementation and adoption of a Military eHealth Information Network (MeHIN). Each country implementation plan followed a structured process using international eHealth standards. DHAPP worked with the private sector to develop a commercial-off-the-shelf (COTS) electronic medical record (EMR) for the collection of data, including patient demographic information, clinical notes for general medical care, HIV encounters, voluntary medical male circumcision, and tuberculosis screening information. The COTS software approach provided a zero-dollar software license and focused on sharing a single version of the EMR across countries, so that all countries could benefit from software enhancements and new features over time. DHAPP also worked with the public sector to modify open source disease surveillance tools and open access of HIV training materials. Important lessons highlight challenges to eHealth implementation, including a paucity of technology infrastructure, military leadership rotations, and the need for basic computer skills building. While not simple, eHealth systems can be built and maintained with requisite security, flexibility, and reporting capabilities that provide critical information to improve the health of individuals and organizations. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Expecting the Unexpected: Radiation Hardened Software

NASA Technical Reports Server (NTRS)

Penix, John; Mehlitz, Peter C.

2005-01-01

Radiation induced Single Event Effects (SEEs) are a serious problem for spacecraft flight software, potentially leading to a complete loss of mission. Conventional risk mitigation has been focused on hardware, leading to slow, expensive and outdated on-board computing devices, increased power consumption and launch mass. Our approach is to look at SEEs from a software perspective, and to explicitly design flight software so that it can detect and correct the majority of SEES. Radiation hardened flight software will reduce the significant residual residual risk for critical missions and flight phases, and enable more use of inexpensive and fast COTS hardware.

Remote imagery for unmanned ground vehicles: the future of path planning for ground robotics

NASA Astrophysics Data System (ADS)

Frederick, Philip A.; Theisen, Bernard L.; Ward, Derek

2006-10-01

Remote Imagery for Unmanned Ground Vehicles (RIUGV) uses a combination of high-resolution multi-spectral satellite imagery and advanced commercial off-the-self (COTS) object-oriented image processing software to provide automated terrain feature extraction and classification. This information, along with elevation data, infrared imagery, a vehicle mobility model and various meta-data (local weather reports, Zobler Soil map, etc...), is fed into automated path planning software to provide a stand-alone ability to generate rapidly updateable dynamic mobility maps for Manned or Unmanned Ground Vehicles (MGVs or UGVs). These polygon based mobility maps can reside on an individual platform or a tactical network. When new information is available, change files are generated and ingested into existing mobility maps based on user selected criteria. Bandwidth concerns are mitigated by the use of shape files for the representation of the data (e.g. each object in the scene is represented by a shape file and thus can be transmitted individually). User input (desired level of stealth, required time of arrival, etc...) determines the priority in which objects are tagged for updates. This paper will also discuss the planned July 2006 field experiment.

NASA Mission Operations Directorate Preparations for the COTS Visiting Vehicles

NASA Technical Reports Server (NTRS)

Shull, Sarah A.; Peek, Kenneth E.

2011-01-01

With the retirement of the Space Shuttle looming, a series of new spacecraft is under development to assist in providing for the growing logistical needs of the International Space Station (ISS). Two of these vehicles are being built under a NASA initiative known as the Commercial Orbital Transportation Services (COTS) program. These visiting vehicles ; Space X s Dragon and Orbital Science Corporation s Cygnus , are to be domestically produced in the United States and designed to add to the capabilities of the Russian Progress and Soyuz workhorses, the European Automated Transfer Vehicle (ATV) and the Japanese H-2 Transfer Vehicle (HTV). Most of what is known about the COTS program has focused on the work of Orbital and SpaceX in designing, building, and testing their respective launch and cargo vehicles. However, there is also a team within the Mission Operations Directorate (MOD) at NASA s Johnson Space Center working with their operational counterparts in these companies to provide operational safety oversight and mission assurance via the development of operational scenarios and products needed for these missions. Ensuring that the operational aspect is addressed for the initial demonstration flights of these vehicles is the topic of this paper. Integrating Dragon and Cygnus into the ISS operational environment has posed a unique challenge to NASA and their partner companies. This is due in part to the short time span of the COTS program, as measured from initial contract award until first launch, as well as other factors that will be explored in the text. Operational scenarios and products developed for each COTS vehicle will be discussed based on the following categories: timelines, on-orbit checkout, ground documentation, crew procedures, software updates and training materials. Also addressed is an outline of the commonalities associated with the operations for each vehicle. It is the intent of the authors to provide their audience with a better understanding of the mission assurance that MOD brings to commercial ventures to the ISS

Commercial Off-The-Shelf (COTS) Graphics Processing Board (GPB) Radiation Test Evaluation Report

NASA Technical Reports Server (NTRS)

Salazar, George A.; Steele, Glen F.

2013-01-01

Large round trip communications latency for deep space missions will require more onboard computational capabilities to enable the space vehicle to undertake many tasks that have traditionally been ground-based, mission control responsibilities. As a result, visual display graphics will be required to provide simpler vehicle situational awareness through graphical representations, as well as provide capabilities never before done in a space mission, such as augmented reality for in-flight maintenance or Telepresence activities. These capabilities will require graphics processors and associated support electronic components for high computational graphics processing. In an effort to understand the performance of commercial graphics card electronics operating in the expected radiation environment, a preliminary test was performed on five commercial offthe- shelf (COTS) graphics cards. This paper discusses the preliminary evaluation test results of five COTS graphics processing cards tested to the International Space Station (ISS) low earth orbit radiation environment. Three of the five graphics cards were tested to a total dose of 6000 rads (Si). The test articles, test configuration, preliminary results, and recommendations are discussed.

Improved Airborne System for Sensing Wildfires

NASA Technical Reports Server (NTRS)

McKeown, Donald; Richardson, Michael

2008-01-01

The Wildfire Airborne Sensing Program (WASP) is engaged in a continuing effort to develop an improved airborne instrumentation system for sensing wildfires. The system could also be used for other aerial-imaging applications, including mapping and military surveillance. Unlike prior airborne fire-detection instrumentation systems, the WASP system would not be based on custom-made multispectral line scanners and associated custom- made complex optomechanical servomechanisms, sensors, readout circuitry, and packaging. Instead, the WASP system would be based on commercial off-the-shelf (COTS) equipment that would include (1) three or four electronic cameras (one for each of three or four wavelength bands) instead of a multispectral line scanner; (2) all associated drive and readout electronics; (3) a camera-pointing gimbal; (4) an inertial measurement unit (IMU) and a Global Positioning System (GPS) receiver for measuring the position, velocity, and orientation of the aircraft; and (5) a data-acquisition subsystem. It would be necessary to custom-develop an integrated sensor optical-bench assembly, a sensor-management subsystem, and software. The use of mostly COTS equipment is intended to reduce development time and cost, relative to those of prior systems.

The Federal Aviation Administration Plan for Research, Engineering and Development, 1994

DTIC Science & Technology

1994-05-01

Aeronautical Data Link Communications and (COTS) runway incursion system software will Applications, and 051-130 Airport Safety be demonstrated as a... airport departure and ar- efforts rival scheduling plans that optimize daily traffic flows for long-range flights between major city- * OTFP System to...Expanded HARS planning capabilities to in- aviation dispatchers to develop optimized high clude enhanced communications software for altitude flight

Case Study of the Space Shuttle Cockpit Avionics Upgrade Software

NASA Technical Reports Server (NTRS)

Ferguson, Roscoe C.; Thompson, Hiram C.

2005-01-01

The purpose of the Space Shuttle Cockpit Avionics Upgrade project was to reduce crew workload and improve situational awareness. The upgrade was to augment the Shuttle avionics system with new hardware and software. An early version of this system was used to gather human factor statistics in the Space Shuttle Motion Simulator of the Johnson Space Center for one month by multiple teams of astronauts. The results were compiled by NASA Ames Research Center and it was was determined that the system provided a better than expected increase in situational awareness and reduction in crew workload. Even with all of the benefits nf the system, NASA cancelled the project towards the end of the development cycle. A major success of this project was the validation of the hardware architecture and software design. This was significant because the project incorporated new technology and approaches for the development of human rated space software. This paper serves as a case study to document knowledge gained and techniques that can be applied for future space avionics development efforts. The major technological advances were the use of reflective memory concepts for data acquisition and the incorporation of Commercial off the Shelf (COTS) products in a human rated space avionics system. The infused COTS products included a real time operating system, a resident linker and loader, a display generation tool set, and a network data manager. Some of the successful design concepts were the engineering of identical outputs in multiple avionics boxes using an event driven approach and inter-computer communication, a reconfigurable data acquisition engine, the use of a dynamic bus bandwidth allocation algorithm. Other significant experiences captured were the use of prototyping to reduce risk, and the correct balance between Object Oriented and Functional based programming.

Current Radiation Issues for Programmable Elements and Devices

NASA Technical Reports Server (NTRS)

Katz, R.; Wang, J. J.; Koga, R.; LaBel, A.; McCollum, J.; Brown, R.; Reed, R. A.; Cronquist, B.; Crain, S.; Scott, T.;

Cancer Osaka thyroid (Cot) phosphorylates Polo-like kinase (PLK1) at Ser137 but not at Thr210.

PubMed

Wu, Binhui; Jiang, Ping; Mu, Yuguang; Wilmouth, Rupert C

2009-12-01

Cancer Osaka thyroid (Cot) is a proto-oncogenic kinase which belongs to the MAP3K family. A peptide-based substrate screening assay revealed that Cot has the ability to phosphorylate Polo-like kinase 1 (Plk1) at Ser137. Kinase assays with intact Plk1 and peptides surrounding Ser137 and Thr210 indicated further that Cot phosphorylates Ser137 but not Thr210. Additional support came from 3D peptide structure prediction and Cot-Plk1 interaction modeling. In vivo experiments demonstrated that wild type Cot, but not a kinase-dead mutant, has the ability to phosphorylate Ser137. Knockdown of Cot in Hela showed a reduction in the level of phosphorylation of Ser137. These results imply for the first time that Cot might be an upstream kinase of Plk1 and suggest a new mechanism for the regulation of the cellular function of Plk1.

Federal Aviation Administration Plan for Research, Engineering and Development 1993

DTIC Science & Technology

1994-02-01

pace the United States economy. With no additional with technology, and help maintain economic major airports planned in the near term, the FAA growth...provides Route Software Development, 62-20 Terminal ARTCC and TRACON controllers with automa- ATC Automation (TATCA), 62-21 Airport Sur- tion aids for...Applications, and 051-130 Airport Safety (COTS) runway incursion system software will Technology. Capital Investment Plan projects: be demonstrated

Building a Trusted Path for Applications Using COTS Components

DTIC Science & Technology

2004-11-01

against attacks by malicious software. Trojan horse programs, i.e., programs with additional hidden, often malicious, functions, are more and more...cannot be imitated by untrusted software." Wiseman et al. (1988) propose a user interface for the SMITE system to prevent Trojan horses from...input, two of which can also be used for the hologram service. 7.0 CONCLUSION Trojan horse programs, i.e., programs with additional hidden, often

Chronic Opioid Therapy and Central Sensitization in Sickle Cell Disease

PubMed Central

Carroll, C. Patrick; Lanzkron, Sophie; Haywood, Carlton; Kiley, Kasey; Pejsa, Megan; Moscou-Jackson, Gyasi; Haythornthwaite, Jennifer A.; Campbell, Claudia M.

2016-01-01

Chronic opioid therapy (COT) for chronic non-cancer pain is frequently debated, and its effectiveness is unproven in sickle cell disease (SCD). The authors conducted a descriptive study among 83 adult SCD patients and compared severity of disease and pain symptoms among those who were prescribed COT (n=29) with those who were not using COT. All patients completed baseline laboratory pain assessment and questionnaires between January 2010 and June 2014. Thereafter, participants recorded daily pain, crises, function, and healthcare utilization for 90 days using electronic diaries. Analyses were conducted shortly after the final diary data collection period. Patients on COT did not differ on age, sex, or measures of disease severity. However, patients on COT exhibited greater levels of clinical pain (particularly non-crisis), central sensitization, depression, and increased diary measures of pain severity, function, and healthcare utilization on crisis and non-crisis diary days, as well as a greater proportion of days in crisis. Including depressive symptoms in multivariate models did not change the associations between COT and pain, interference, central sensitization, or utilization. Additionally, participants not on COT displayed the expected positive relationship between central sensitization and clinical pain, whereas those on COT demonstrated no such relationship, despite having both higher central sensitization and higher clinical pain. Overall, the results point out a high symptom burden in SCD patients on COT, including those on high-dose COT, and suggest that nociceptive processing in SCD patients on COT differs from those who are not. PMID:27320469

Software Model Checking Without Source Code

NASA Technical Reports Server (NTRS)

Chaki, Sagar; Ivers, James

2009-01-01

We present a framework, called AIR, for verifying safety properties of assembly language programs via software model checking. AIR extends the applicability of predicate abstraction and counterexample guided abstraction refinement to the automated verification of low-level software. By working at the assembly level, AIR allows verification of programs for which source code is unavailable-such as legacy and COTS software-and programs that use features-such as pointers, structures, and object-orientation-that are problematic for source-level software verification tools. In addition, AIR makes no assumptions about the underlying compiler technology. We have implemented a prototype of AIR and present encouraging results on several non-trivial examples.

Kennedy Space Center's Command and Control System - "Toasters to Rocket Ships"

NASA Technical Reports Server (NTRS)

Lougheed, Kirk; Mako, Cheryle

2011-01-01

This slide presentation reviews the history of the development of the command and control system at Kennedy Space Center. From a system that could be brought to Florida in the trunk of a car in the 1950's. Including the development of larger and more complex launch vehicles with the Apollo program where human launch controllers managed the launch process with a hardware only system that required a dedicated human interface to perform every function until the Apollo vehicle lifted off from the pad. Through the development of the digital computer that interfaced with ground launch processing systems with the Space Shuttle program. Finally, showing the future control room being developed to control the missions to return to the moon and Mars, which will maximize the use of Commercial-Off-The Shelf (COTS) hardware and software which was standards based and not tied to a single vendor. The system is designed to be flexible and adaptable to support the requirements of future spacecraft and launch vehicles.

Qualification Tests of Micro-camera Modules for Space Applications

NASA Astrophysics Data System (ADS)

Kimura, Shinichi; Miyasaka, Akira

Visual capability is very important for space-based activities, for which small, low-cost space cameras are desired. Although cameras for terrestrial applications are continually being improved, little progress has been made on cameras used in space, which must be extremely robust to withstand harsh environments. This study focuses on commercial off-the-shelf (COTS) CMOS digital cameras because they are very small and are based on an established mass-market technology. Radiation and ultrahigh-vacuum tests were conducted on a small COTS camera that weighs less than 100 mg (including optics). This paper presents the results of the qualification tests for COTS cameras and for a small, low-cost COTS-based space camera.

A high-rate PCI-based telemetry processor system

NASA Astrophysics Data System (ADS)

Turri, R.

2002-07-01

The high performances reached by the Satellite on-board telemetry generation and transmission, as consequently, will impose the design of ground facilities with higher processing capabilities at low cost to allow a good diffusion of these ground station. The equipment normally used are based on complex, proprietary bus and computing architectures that prevent the systems from exploiting the continuous and rapid increasing in computing power available on market. The PCI bus systems now allow processing of high-rate data streams in a standard PC-system. At the same time the Windows NT operating system supports multitasking and symmetric multiprocessing, giving the capability to process high data rate signals. In addition, high-speed networking, 64 bit PCI-bus technologies and the increase in processor power and software, allow creating a system based on COTS products (which in future may be easily and inexpensively upgraded). In the frame of EUCLID RTP 9.8 project, a specific work element was dedicated to develop the architecture of a system able to acquire telemetry data of up to 600 Mbps. Laben S.p.A - a Finmeccanica Company -, entrusted of this work, has designed a PCI-based telemetry system making possible the communication between a satellite down-link and a wide area network at the required rate.

Commercial Off-the-Shelf (COTS) Components and Enterprise Component Information System (eCIS)

DOE Office of Scientific and Technical Information (OSTI.GOV)

John Minihan; Ed Schmidt; Greg Enserro

The purpose of the project was to develop the processes for using commercial off-the-shelf (COTS) parts for WR production and to put in place a system for implementing the data management tools required to disseminate, store, track procurement, and qualify vendors. Much of the effort was devoted to determining if the use of COTS parts was possible. A basic question: How does the Nuclear Weapons Complex (NWC) begin to use COTS in the weapon Stockpile Life Extension Programs with high reliability, affordability, while managing risk at acceptable levels? In FY00, it was determined that a certain weapon refurbishment program couldmore » not be accomplished without the use of COTS components. The elements driving the use of COTS components included decreased cost, greater availability, and shorter delivery time. Key factors that required implementation included identifying the best suppliers and components, defining life cycles and predictions of obsolescence, testing the feasibility of using COTS components with a test contractor to ensure capability, as well as quality and reliability, and implementing the data management tools required to disseminate, store, track procurement, and qualify vendors. The primary effort of this project then was to concentrate on the risks involved in the use of COTS and address the issues of part and vendor selection, procurement and acceptance processes, and qualification of the parts via part and sample testing. The Enterprise Component Information System (eCIS) was used to manage the information generated by the COTS process. eCIS is a common interface for both the design and production of NWC components and systems integrating information between SNL National Laboratory (SNL) and the Kansas City Plant (KCP). The implementation of COTS components utilizes eCIS from part selection through qualification release. All part related data is linked across an unclassified network for access by both SNL and KCP personnel. The system includes not only NWC part information but also includes technical reference data for over 25 Million electronic and electromechanical commercial and military parts via a data subscription. With the capabilities added to the system through this project, eCIS provides decision support, parts list/BOM analysis, editing, tracking, workflows, reporting, and history/legacy information integrating manufacturer reference, company technical, company business, and design data.« less

Chronic Opioid Therapy and Central Sensitization in Sickle Cell Disease.

PubMed

Carroll, C Patrick; Lanzkron, Sophie; Haywood, Carlton; Kiley, Kasey; Pejsa, Megan; Moscou-Jackson, Gyasi; Haythornthwaite, Jennifer A; Campbell, Claudia M

2016-07-01

Chronic opioid therapy (COT) for chronic non-cancer pain is frequently debated, and its effectiveness is unproven in sickle cell disease (SCD). The authors conducted a descriptive study among 83 adult SCD patients and compared the severity of disease and pain symptoms among those who were prescribed COT (n=29) with those who were not using COT. All patients completed baseline laboratory pain assessment and questionnaires between January 2010 and June 2014. Thereafter, participants recorded daily pain, crises, function, and healthcare utilization for 90 days using electronic diaries. Analyses were conducted shortly after the final diary data collection period. Patients on COT did not differ on age, sex, or measures of disease severity. However, patients on COT exhibited greater levels of clinical pain (particularly non-crisis); central sensitization; and depression and increased diary measures of pain severity, function, and healthcare utilization on crisis and non-crisis diary days, as well as a greater proportion of days in crisis. Including depressive symptoms in multivariate models did not change the associations between COT and pain, interference, central sensitization, or utilization. Additionally, participants not on COT displayed the expected positive relationship between central sensitization and clinical pain, whereas those on COT demonstrated no such relationship, despite having both higher central sensitization and higher clinical pain. Overall, the results point out a high symptom burden in SCD patients on COT, including those on high-dose COT, and suggest that nociceptive processing in SCD patients on COT differs from those who are not. Copyright © 2016 American Journal of Preventive Medicine. Published by Elsevier Inc. All rights reserved.

Performance Improvements to the Naval Postgraduate School Turbopropulsion Labs Transonic Axially Splittered Rotor

DTIC Science & Technology

2013-12-01

Implementation of current NPS TPL design procedure that uses COTS software (MATLAB, SolidWorks, and ANSYS - CFX ) for the geometric rendering and...procedure that uses commercial-off-the-shelf software (MATLAB, SolidWorks, and ANSYS - CFX ) for the geometric rendering and analysis was modified and... CFX The CFD simulation program in ANSYS Workbench. CFX -Pre CFX boundary conditions and solver settings module. CFX -Solver CFX solver program. CFX

Improvements to the Processing and Characterization of Needled Composite Laminates

DTIC Science & Technology

2014-01-01

the automated processing equipment are shown and discussed. The modifications allow better spatial control at the penetration sites and the ability... automated processing equipment are shown and discussed. The modifications allow better spatial control at the penetration sites and the ability to...semi- automated processing equipment, commercial off-the-shelf (COTS) needles and COTS aramid mat designed for other applications. Needled material

Integration of an open interface PC scene generator using COTS DVI converter hardware

NASA Astrophysics Data System (ADS)

Nordland, Todd; Lyles, Patrick; Schultz, Bret

2006-05-01

Commercial-Off-The-Shelf (COTS) personal computer (PC) hardware is increasingly capable of computing high dynamic range (HDR) scenes for military sensor testing at high frame rates. New electro-optical and infrared (EO/IR) scene projectors feature electrical interfaces that can accept the DVI output of these PC systems. However, military Hardware-in-the-loop (HWIL) facilities such as those at the US Army Aviation and Missile Research Development and Engineering Center (AMRDEC) utilize a sizeable inventory of existing projection systems that were designed to use the Silicon Graphics Incorporated (SGI) digital video port (DVP, also known as DVP2 or DD02) interface. To mate the new DVI-based scene generation systems to these legacy projection systems, CG2 Inc., a Quantum3D Company (CG2), has developed a DVI-to-DVP converter called Delta DVP. This device takes progressive scan DVI input, converts it to digital parallel data, and combines and routes color components to derive a 16-bit wide luminance channel replicated on a DVP output interface. The HWIL Functional Area of AMRDEC has developed a suite of modular software to perform deterministic real-time, wave band-specific rendering of sensor scenes, leveraging the features of commodity graphics hardware and open source software. Together, these technologies enable sensor simulation and test facilities to integrate scene generation and projection components with diverse pedigrees.

Firefly: A HOT camera core for thermal imagers with enhanced functionality

NASA Astrophysics Data System (ADS)

Pillans, Luke; Harmer, Jack; Edwards, Tim

2015-06-01

Raising the operating temperature of mercury cadmium telluride infrared detectors from 80K to above 160K creates new applications for high performance infrared imagers by vastly reducing the size, weight and power consumption of the integrated cryogenic cooler. Realizing the benefits of Higher Operating Temperature (HOT) requires a new kind of infrared camera core with the flexibility to address emerging applications in handheld, weapon mounted and UAV markets. This paper discusses the Firefly core developed to address these needs by Selex ES in Southampton UK. Firefly represents a fundamental redesign of the infrared signal chain reducing power consumption and providing compatibility with low cost, low power Commercial Off-The-Shelf (COTS) computing technology. This paper describes key innovations in this signal chain: a ROIC purpose built to minimize power consumption in the proximity electronics, GPU based image processing of infrared video, and a software customisable infrared core which can communicate wirelessly with other Battlespace systems.

An adaptable product for material processing and life science missions

NASA Technical Reports Server (NTRS)

Wassick, Gregory; Dobbs, Michael

1995-01-01

The Experiment Control System II (ECS-II) is designed to make available to the microgravity research community the same tools and mode of automated experimentation that their ground-based counterparts have enjoyed for the last two decades. The design goal was accomplished by combining commercial automation tools familiar to the experimenter community with system control components that interface with the on-orbit platform in a distributed architecture. The architecture insulates the tools necessary for managing a payload. By using commercial software and hardware components whenever possible, development costs were greatly reduced when compared to traditional space development projects. Using commercial-off-the-shelf (COTS) components also improved the usability documentation, and reducing the need for training of the system by providing familiar user interfaces, providing a wealth of readily available documentation, and reducing the need for training on system-specific details. The modularity of the distributed architecture makes it very amenable for modification to different on-orbit experiments requiring robotics-based automation.

Case for Deploying Complex Systems Utilizing Commodity Components

NASA Technical Reports Server (NTRS)

Bryant, Barry S.; Pitts, R. Lee

2003-01-01

When the International Space Station (ISS) finally reached an operational state, many of the Payload Operations and Integration Facility (POIF) hardware components were reaching end of life, COTS product costs were soaring, and the ISS budget was becoming severely constrained. However, most requirement development was complete. In addition, the ISS program is a fully functioning program with at least fifteen years of operational life remaining. Therefore it is critical that any upgrades, refurbishments, or enhancements be accomplished in realtime with minimal disruptions to service. For these and other reasons, it was necessary to ensure the viability of the POIF. Due to the to the breadth of capability of the POIF (a NASA ground station), it is believed that the lessons to be learned by other complex systems are applicable and any solutions garnered by the POIF are applicable to other complex systems as well. With that in mind, a number of new approaches have been investigated to increase the portability of the POIF and reduce the cost of refurbishment, operations, and maintenance. These new approaches were directed at the Total Cost of Ownership (TCO); not only the refurbishment but also current operational difficulties, licensing, and anticipation of the next refurbishment. Our basic premise is that technology had evolved dramatically since the concept of the POIF ground system and we should leverage our experience on this new technological landscape. Fortunately, Moore's law and market forces have changed the landscape considerably. These changes are manifest in five (5) ways that are particularly relevant to POIF: 1. Complex Instruction Set Computing (CISC) processors have advanced to unprecedented levels of compute capacity with a dramatic cost break, 2. Linux has become a major operating system supported by most vendors on a broad range of platforms, 3. Windows(TradeMark) based desktops are pervasive in the office environment, 4. Stable and affordable WindowsTM development environments and tools are available and offer a rich set of capabilities, 5. The WindowsTM 2000 provides a stable client platform, Therefore, five studies were proposed, developed, and are in the current process of deployment which dramatically reduces the cost of operations, maintenance, refurbishment, and deployment of a ground system. Restating and refining the basic premise stated earlier, it is possible to enhance operations through the replacement of hardware and software components with commodity based items wherever applicable. This will dramatically reduce the overall lifecycle cost of the project. The first study leveraged the POIF S secure, three-tier, web architecture to replace the client workstations with lower cost PC platforms. A second study initiated a review of COTS products to examine the level of added value of each product. This study included replacement of some COTS products with custom code, deletions, substitutions, and consolidation of COTS products. Studies three and four reviewed the server architectures of the data distribution systems and Enhanced HOSC System (EHS) command and telemetry system to propose migration to new platforms, both software and hardware. The final study reviewed current IP communication technologies, developed an operational model for flight operations, and demonstrated that voice over IP was practical and could be integrated into operations.

Lower crustal strength controls on melting and type of oceanization at magma-poor margins

NASA Astrophysics Data System (ADS)

Ros, E.; Perez-Gussinye, M.; Araujo, M. N.; Thoaldo Romeiro, M.; Andres-Martinez, M.; Morgan, J. P.

2017-12-01

Geodynamical models have been widely used to explain the variability in the architectonical style of conjugate rifted margins as a combination of lithospheric deformation modes, which are strongly influenced by lower crustal strength. We use 2D numerical models to show that the lower crustal strength also plays a key role on the onset and amount of melting and serpentinization during continental rifting. The relative timing between melting and serpentinization onsets controls whether the continent-ocean transition (COT) of margins will be predominantly magmatic or will mainly consist of exhumed and serpentinized mantle. Based on our results for magma-poor continental rifting, we propose a genetic link between margin architecture and COT styles that can be used as an additional tool to help interpret and understand the processes leading to margin formation. Our results show that strong lower crusts and very slow extension velocities (<5 mm/yr) lead to either symmetric or asymmetric margins with large oceanward dipping faults, strong syn-rift subsidence and abrupt crustal tapering beneath the continental shelf. These margins are characterized by a COT consisting of exhumed and serpentinized mantle and some magmatic products. Weak lower crusts at ultra-slow velocities lead also to either symmetric or asymmetric margins with small faults dipping both ocean- and landward, small syn-rift subsidence and gentle crustal tapering, and present a predominantly magmatic COT, perhaps underlain by some serpentinized mantle. When conjugate margins are asymmetric, if the rheology is relatively strong, serpentinite predominantly underlays the wide margin, whereas if the lower crustal strength is weak, melt preferentially migrates towards the wide margin. Based on the onshore lithospheric structure, extension velocity and margin architecture of the magma-poor section of the South Atlantic, we suggest that the COT of the northern sector, Camamu-Gabon basins, is more likely to consist of exhumed mantle with intruded magmatism, while to the South, the Camamu-Kwanza and North Santos-South Kwanza conjugates, may be better characterized by a predominantly magmatic COT.

Cot, a novel kinase of histone H3, induces cellular transformation through up-regulation of c-fos transcriptional activity.

PubMed

Choi, Hong Seok; Kang, Bong Seok; Shim, Jung-Hyun; Cho, Yong-Yeon; Choi, Bu Young; Bode, Ann M; Dong, Zigang

2008-01-01

Post-translational modification of histones is critical for gene expression, mitosis, cell growth, apoptosis, and cancer development. Thus, finding protein kinases that are responsible for the phosphorylation of histones at critical sites is considered an important step in understanding the process of histone modification. The serine/threonine kinase Cot is a member of the mitogen-activated protein kinase (MAPK) kinase kinase family. We show here that Cot can phosphorylate histone H3 at Ser-10 in vivo and in vitro, and that the phosphorylation of histone H3 at Ser-10 is required for Cot-induced cell transformation. We found that activated Cot is recruited to the c-fos promoter resulting in increased activator protein-1 (AP-1) transactivation. The formation of the Cot-c-fos promoter complex was also apparent when histone H3 was phosphorylated at Ser-10. Furthermore, the use of dominant negative mutants of histone H3 revealed that Cot was required for phosphorylation of histone H3 at Ser-10 to induce neoplastic cell transformation. These results revealed an important function of Cot as a newly discovered histone H3 kinase. Moreover, the transforming ability of Cot results from the coordinated activation of histone H3, which ultimately converges on the regulation of the transcriptional activity of the c-fos promoter, followed by AP-1 transactivation activity.

Multipoint fiber-optic laser-ultrasonic actuator based on fiber core-opened tapers.

PubMed

Tian, Jiajun; Dong, Xiaolong; Gao, Shimin; Yao, Yong

2017-11-27

In this study, a novel fiber-optic, multipoint, laser-ultrasonic actuator based on fiber core-opened tapers (COTs) is proposed and demonstrated. The COTs were fabricated by splicing single-mode fibers using a standard fiber splicer. A COT can effectively couple part of a core mode into cladding modes, and the coupling ratio can be controlled by adjusting the taper length. Such characteristics are used to obtain a multipoint, laser-ultrasonic actuator with balanced signal strength by reasonably controlling the taper lengths of the COTs. As a prototype, we constructed an actuator that generated ultrasound at four points with a balanced ultrasonic strength by connecting four COTs with coupling ratios of 24.5%, 33.01%, 49.51%, and 87.8% in a fiber link. This simple-to-fabricate, multipoint, laser-ultrasonic actuator with balanced ultrasound signal strength has potential applications in fiber-optic ultrasound testing technology.

Initial validation of the prekindergarten Classroom Observation Tool and goal setting system for data-based coaching.

PubMed

Crawford, April D; Zucker, Tricia A; Williams, Jeffrey M; Bhavsar, Vibhuti; Landry, Susan H

2013-12-01

Although coaching is a popular approach for enhancing the quality of Tier 1 instruction, limited research has addressed observational measures specifically designed to focus coaching on evidence-based practices. This study explains the development of the prekindergarten (pre-k) Classroom Observation Tool (COT) designed for use in a data-based coaching model. We examined psychometric characteristics of the COT and explored how coaches and teachers used the COT goal-setting system. The study included 193 coaches working with 3,909 pre-k teachers in a statewide professional development program. Classrooms served 3 and 4 year olds (n = 56,390) enrolled mostly in Title I, Head Start, and other need-based pre-k programs. Coaches used the COT during a 2-hr observation at the beginning of the academic year. Teachers collected progress-monitoring data on children's language, literacy, and math outcomes three times during the year. Results indicated a theoretically supported eight-factor structure of the COT across language, literacy, and math instructional domains. Overall interrater reliability among coaches was good (.75). Although correlations with an established teacher observation measure were small, significant positive relations between COT scores and children's literacy outcomes indicate promising predictive validity. Patterns of goal-setting behaviors indicate teachers and coaches set an average of 43.17 goals during the academic year, and coaches reported that 80.62% of goals were met. Both coaches and teachers reported the COT was a helpful measure for enhancing quality of Tier 1 instruction. Limitations of the current study and implications for research and data-based coaching efforts are discussed. PsycINFO Database Record (c) 2013 APA, all rights reserved.

High Available COTS Based Computer for Space

NASA Astrophysics Data System (ADS)

Hartmann, J.; Magistrati, Giorgio

2015-09-01

The availability and reliability factors of a system are central requirements of a target application. From a simple fuel injection system used in cars up to a flight control system of an autonomous navigating spacecraft, each application defines its specific availability factor under the target application boundary conditions. Increasing quality requirements on data processing systems used in space flight applications calling for new architectures to fulfill the availability, reliability as well as the increase of the required data processing power. Contrary to the increased quality request simplification and use of COTS components to decrease costs while keeping the interface compatibility to currently used system standards are clear customer needs. Data processing system design is mostly dominated by strict fulfillment of the customer requirements and reuse of available computer systems were not always possible caused by obsolescence of EEE-Parts, insufficient IO capabilities or the fact that available data processing systems did not provide the required scalability and performance.

Fault Injection Campaign for a Fault Tolerant Duplex Framework

NASA Technical Reports Server (NTRS)

Sacco, Gian Franco; Ferraro, Robert D.; von llmen, Paul; Rennels, Dave A.

2007-01-01

Fault tolerance is an efficient approach adopted to avoid or reduce the damage of a system failure. In this work we present the results of a fault injection campaign we conducted on the Duplex Framework (DF). The DF is a software developed by the UCLA group [1, 2] that uses a fault tolerant approach and allows to run two replicas of the same process on two different nodes of a commercial off-the-shelf (COTS) computer cluster. A third process running on a different node, constantly monitors the results computed by the two replicas, and eventually restarts the two replica processes if an inconsistency in their computation is detected. This approach is very cost efficient and can be adopted to control processes on spacecrafts where the fault rate produced by cosmic rays is not very high.

Antibiotic resistant salmonella and Escherichia coli isolated from indigenous Gallus domesticus in Nairobi, Kenya.

PubMed

Wesonga, S M; Muluvi, G M; Okemo, P O; Kariuki, S

2010-05-01

To characterise and investigate antimicrobial resistance of Esherichia coli and salmonella strains isolated from indigenous Gallus gallus in a leading slaughterhouse/market outlet in Nairobi-Kenya. A repeated cross sectional study and based on random sampling was used. The study was carried out in a leading market outlet in Nairobi, Kenya. A hundred and four indigenous chicken rectal swabs were analysed, of which 67.3% were contaminated with Escherichia coli and 12.5% with Salmonella typhimurium. Seventy Escherichia coli isolates showed resistance phenotypes to one, two or more antibiotics. The most common antimicrobial resistance pattern was the single resistance to Tet (21.43%), followed by Amp Cot Tet (14%), Aug Amp Cot Tet (4.29%), Aug Amp Cot Tet Kan Chl (2.86%), Amp Cot Tet Chl, Cot Tet (2.86%) and Crx Amp Cot Tet Chl, Crx Amp Cot Chi, Amp Cot, Aug Amp, (1.43%) respectively. The highest rate of resistance was against Tet (55.7%), followed by Cot (40%). Third in line of resistance was Amp 32.86%, followed by Aug (11.43%), low or moderate resistance was against Chl (8.57%), Kan (4.29%), and Crx (2.86%) (P<0.0002). Salmonella typhimurium recovered displayed single resistance pattern to Tet (16.67%), Gen Cot Tet (8.33%), Amp Cot Tet (8.33%), Aug Amp Cot Tet (8.33%) and Amp Cot Tet Chl (16.67%). The highest resistance was against Tet (58.3%), Cot (41.7%), Amp (33.3%), Chl (16.7%), Aug and Gen (8.3%) respectively (P<0.0001). 3.0kb and 5.6kb plasmids isolated were not transferable by conjugation. Routine surveillance at slaughter/market outlets of Escherichia coli and Salmonella enterica should be done to identify infected flocks as a regulatory procedure for food safety and security programme.

UAF: a generic OPC unified architecture framework

NASA Astrophysics Data System (ADS)

Pessemier, Wim; Deconinck, Geert; Raskin, Gert; Saey, Philippe; Van Winckel, Hans

2012-09-01

As an emerging Service Oriented Architecture (SOA) specically designed for industrial automation and process control, the OPC Unied Architecture specication should be regarded as an attractive candidate for controlling scientic instrumentation. Even though an industry-backed standard such as OPC UA can oer substantial added value to these projects, its inherent complexity poses an important obstacle for adopting the technology. Building OPC UA applications requires considerable eort, even when taking advantage of a COTS Software Development Kit (SDK). The OPC Unied Architecture Framework (UAF) attempts to reduce this burden by introducing an abstraction layer between the SDK and the application code in order to achieve a better separation of the technical and the functional concerns. True to its industrial origin, the primary requirement of the framework is to maintain interoperability by staying close to the standard specications, and by expecting the minimum compliance from other OPC UA servers and clients. UAF can therefore be regarded as a software framework to quickly and comfortably develop and deploy OPC UA-based applications, while remaining compatible to third party OPC UA-compliant toolkits, servers (such as PLCs) and clients (such as SCADA software). In the rst phase, as covered by this paper, only the client-side of UAF has been tackled in order to transparently handle discovery, session management, subscriptions, monitored items etc. We describe the design principles and internal architecture of our open-source software project, the rst results of the framework running at the Mercator Telescope, and we give a preview of the planned server-side implementation.

Rhipicephalus microplus dataset of nonredundant raw sequence reads from 454 GS FLX sequencing of Cot-selected (Cot = 660) genomic DNA

USDA-ARS?s Scientific Manuscript database

A reassociation kinetics-based approach was used to reduce the complexity of genomic DNA from the Deutsch laboratory strain of the cattle tick, Rhipicephalus microplus, to facilitate genome sequencing. Selected genomic DNA (Cot value = 660) was sequenced using 454 GS FLX technology, resulting in 356...

Programmable personality interface for the dynamic infrared scene generator (IRSG2)

NASA Astrophysics Data System (ADS)

Buford, James A., Jr.; Mobley, Scott B.; Mayhall, Anthony J.; Braselton, William J.

1998-07-01

As scene generator platforms begin to rely specifically on commercial off-the-shelf (COTS) hardware and software components, the need for high speed programmable personality interfaces (PPIs) are required for interfacing to Infrared (IR) flight computer/processors and complex IR projectors in the hardware-in-the-loop (HWIL) simulation facilities. Recent technological advances and innovative applications of established technologies are beginning to allow development of cost effective PPIs to interface to COTS scene generators. At the U.S. Army Aviation and Missile Command (AMCOM) Missile Research, Development, and Engineering Center (MRDEC) researchers have developed such a PPI to reside between the AMCOM MRDEC IR Scene Generator (IRSG) and either a missile flight computer or the dynamic Laser Diode Array Projector (LDAP). AMCOM MRDEC has developed several PPIs for the first and second generation IRSGs (IRSG1 and IRSG2), which are based on Silicon Graphics Incorporated (SGI) Onyx and Onyx2 computers with Reality Engine 2 (RE2) and Infinite Reality (IR/IR2) graphics engines. This paper provides an overview of PPIs designed, integrated, tested, and verified at AMCOM MRDEC, specifically the IRSG2's PPI.

Goal Structuring Notation in a Radiation Hardening Assurance Case for COTS-Based Spacecraft

NASA Technical Reports Server (NTRS)

Witulski, A.; Austin, R.; Evans, J.; Mahadevan, N.; Karsai, G.; Sierawski, B.; LaBel, K.; Reed, R.

2016-01-01

The attached presentation is a summary of how mission assurance is supported by model-based representations of spacecraft systems that can define sub-system functionality and interfacing, reliability parameters, as well as detailing a new paradigm for assurance, a model-centric and not document-centric process.

Cot/tpl2 (MAP3K8) mediates myeloperoxidase activity and hypernociception following peripheral inflammation.

PubMed

Soria-Castro, Irene; Krzyzanowska, Agnieszka; Pelaéz, Marta López; Regadera, Javier; Ferrer, Gema; Montoliu, Lluis; Rodríguez-Ramos, Rosario; Fernández, Margarita; Alemany, Susana

2010-10-29

Cot/tpl2 (also known as MAP3K8) has emerged as a new and potentially interesting therapeutic anti-inflammatory target. Here, we report the first study of Cot/tpl2 involvement in acute peripheral inflammation in vivo. Six hours after an intraplantar injection of zymosan, Cot/tpl2(-/-) mice showed a 47% reduction in myeloperoxidase activity, concomitant with a 46% lower neutrophil recruitment and a 40% decreased luminol-mediated bioluminescence imaging in vivo. Accordingly, Cot/tpl2 deficiency provoked a 25-30% reduction in luminol-mediated bioluminescence and neutrophil recruitment together with a 65% lower macrophage recruitment 4 h following zymosan-induced peritonitis. Significantly impaired levels of G-CSF and GM-CSF and of other cytokines such as TNFα, IL-1β, and IL-6, as well as some chemokines such as MCP-1, MIP-1β, and keratinocyte-derived chemokine, were detected during the acute zymosan-induced intraplantar inflammatory response in Cot/tpl2(-/-) mice. Moreover, Cot/tpl2 deficiency dramatically decreased the production of the hypernociceptive ligand NGF at the inflammatory site during the course of inflammation. Most importantly, Cot/tpl2 deficiency significantly reduced zymosan-induced inflammatory hypernociception in mice, with a most pronounced effect of a 50% decrease compared with wild type (WT) at 24 h following intraplantar injection of zymosan. At this time, Cot/tpl2(-/-) mice showed significantly reduced NGF, TNFα, and prostaglandin E(2) levels compared with WT littermates. In conclusion, our study demonstrates an important role of Cot/tpl2 in the NGF, G-CSF, and GM-CSF production and myeloperoxidase activity in the acute inflammatory response process and its implication in inflammatory hypernociception.

2nd Generation QUATARA Flight Computer Project

NASA Technical Reports Server (NTRS)

Falker, Jay; Keys, Andrew; Fraticelli, Jose Molina; Capo-Iugo, Pedro; Peeples, Steven

2015-01-01

Single core flight computer boards have been designed, developed, and tested (DD&T) to be flown in small satellites for the last few years. In this project, a prototype flight computer will be designed as a distributed multi-core system containing four microprocessors running code in parallel. This flight computer will be capable of performing multiple computationally intensive tasks such as processing digital and/or analog data, controlling actuator systems, managing cameras, operating robotic manipulators and transmitting/receiving from/to a ground station. In addition, this flight computer will be designed to be fault tolerant by creating both a robust physical hardware connection and by using a software voting scheme to determine the processor's performance. This voting scheme will leverage on the work done for the Space Launch System (SLS) flight software. The prototype flight computer will be constructed with Commercial Off-The-Shelf (COTS) components which are estimated to survive for two years in a low-Earth orbit.

Use of COTS Batteries on ISS and Shuttle

NASA Technical Reports Server (NTRS)

Jeevarajan, Judith A.

2004-01-01

This presentation focuses on COTS Battery testing for energy content, toxicity, hazards, failures modes and controls for different battery chemistries. It also discusses the current program requirements, challenges with COTS Batteries in manned vehicle COTS methodology, JSC test details, and gives a list of incidents from consumer protection safety commissions. The Battery test process involved testing new batteries for engineering certification, qualification of batteries, flight acceptance, cell and battery, environment, performance and abuse. Their conclusions and recommendations were that: high risk is undertaken with the use of COTS batteries, hazard control verification is required to allow the use of these batteries on manned space flights, failures during use cannot be understood if different scenarios of failure are not tested on the ground, and that testing is performed on small sample numbers due to restrictions on cost and time. They recommend testing of large sample size to gain more confidence in the operation of the hazard controls.

A Methodology and Implementation for Annotating Digital Images for Context-appropriate Use in an Academic Health Care Environment

PubMed Central

Goede, Patricia A.; Lauman, Jason R.; Cochella, Christopher; Katzman, Gregory L.; Morton, David A.; Albertine, Kurt H.

2004-01-01

Use of digital medical images has become common over the last several years, coincident with the release of inexpensive, mega-pixel quality digital cameras and the transition to digital radiology operation by hospitals. One problem that clinicians, medical educators, and basic scientists encounter when handling images is the difficulty of using business and graphic arts commercial-off-the-shelf (COTS) software in multicontext authoring and interactive teaching environments. The authors investigated and developed software-supported methodologies to help clinicians, medical educators, and basic scientists become more efficient and effective in their digital imaging environments. The software that the authors developed provides the ability to annotate images based on a multispecialty methodology for annotation and visual knowledge representation. This annotation methodology is designed by consensus, with contributions from the authors and physicians, medical educators, and basic scientists in the Departments of Radiology, Neurobiology and Anatomy, Dermatology, and Ophthalmology at the University of Utah. The annotation methodology functions as a foundation for creating, using, reusing, and extending dynamic annotations in a context-appropriate, interactive digital environment. The annotation methodology supports the authoring process as well as output and presentation mechanisms. The annotation methodology is the foundation for a Windows implementation that allows annotated elements to be represented as structured eXtensible Markup Language and stored separate from the image(s). PMID:14527971

Occurrence of Antimicrobial-Resistant Escherichia coli and Salmonella enterica in the Beef Cattle Production and Processing Continuum.

PubMed

Schmidt, John W; Agga, Getahun E; Bosilevac, Joseph M; Brichta-Harhay, Dayna M; Shackelford, Steven D; Wang, Rong; Wheeler, Tommy L; Arthur, Terrance M

2015-01-01

Specific concerns have been raised that third-generation cephalosporin-resistant (3GC(r)) Escherichia coli, trimethoprim-sulfamethoxazole-resistant (COT(r)) E. coli, 3GC(r) Salmonella enterica, and nalidixic acid-resistant (NAL(r)) S. enterica may be present in cattle production environments, persist through beef processing, and contaminate final products. The prevalences and concentrations of these organisms were determined in feces and hides (at feedlot and processing plant), pre-evisceration carcasses, and final carcasses from three lots of fed cattle (n = 184). The prevalences and concentrations were further determined for strip loins from 103 of the carcasses. 3GC(r) Salmonella was detected on 7.6% of hides during processing and was not detected on the final carcasses or strip loins. NAL(r) S. enterica was detected on only one hide. 3GC(r) E. coli and COT(r) E. coli were detected on 100.0% of hides during processing. Concentrations of 3GC(r) E. coli and COT(r) E. coli on hides were correlated with pre-evisceration carcass contamination. 3GC(r) E. coli and COT(r) E. coli were each detected on only 0.5% of final carcasses and were not detected on strip loins. Five hundred and 42 isolates were screened for extraintestinal pathogenic E. coli (ExPEC) virulence-associated markers. Only two COT(r) E. coli isolates from hides were ExPEC, indicating that fed cattle products are not a significant source of ExPEC causing human urinary tract infections. The very low prevalences of these organisms on final carcasses and their absence on strip loins demonstrate that current sanitary dressing procedures and processing interventions are effective against antimicrobial-resistant bacteria. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Hardware Development Process for Human Research Facility Applications

NASA Technical Reports Server (NTRS)

Bauer, Liz

2000-01-01

The simple goal of the Human Research Facility (HRF) is to conduct human research experiments on the International Space Station (ISS) astronauts during long-duration missions. This is accomplished by providing integration and operation of the necessary hardware and software capabilities. A typical hardware development flow consists of five stages: functional inputs and requirements definition, market research, design life cycle through hardware delivery, crew training, and mission support. The purpose of this presentation is to guide the audience through the early hardware development process: requirement definition through selecting a development path. Specific HRF equipment is used to illustrate the hardware development paths. The source of hardware requirements is the science community and HRF program. The HRF Science Working Group, consisting of SCientists from various medical disciplines, defined a basic set of equipment with functional requirements. This established the performance requirements of the hardware. HRF program requirements focus on making the hardware safe and operational in a space environment. This includes structural, thermal, human factors, and material requirements. Science and HRF program requirements are defined in a hardware requirements document which includes verification methods. Once the hardware is fabricated, requirements are verified by inspection, test, analysis, or demonstration. All data is compiled and reviewed to certify the hardware for flight. Obviously, the basis for all hardware development activities is requirement definition. Full and complete requirement definition is ideal prior to initiating the hardware development. However, this is generally not the case, but the hardware team typically has functional inputs as a guide. The first step is for engineers to conduct market research based on the functional inputs provided by scientists. CommerCially available products are evaluated against the science requirements as well as modifications needed to meet program requirements. Options are consolidated and the hardware development team reaches a hardware development decision point. Within budget and schedule constraints, the team must decide whether or not to complete the hardware as an in-house, subcontract with vendor, or commercial-off-the-shelf (COTS) development. An in-house development indicates NASA personnel or a contractor builds the hardware at a NASA site. A subcontract development is completed off-site by a commercial company. A COTS item is a vendor product available by ordering a specific part number. The team evaluates the pros and cons of each development path. For example, in-bouse developments utilize existing corporate knowledge regarding bow to build equipment for use in space. However, technical expertise would be required to fully understand the medical equipment capabilities, such as for an ultrasound system. It may require additional time and funding to gain the expertise that commercially exists. The major benefit of subcontracting a hardware development is the product is delivered as an end-item and commercial expertise is utilized. On the other hand, NASA has limited control over schedule delays. The final option of COTS or modified COTS equipment is a compromise between in-house and subcontracts. A vendor product may exist that meets all functional requirements but req uires in-house modifications for successful operation in a space environment. The HRF utilizes equipment developed using all of the paths described: inhouse, subcontract, and modified COTS.

Aerial release of bacteria from cot mattress materials and the sudden infant death syndrome.

PubMed

Sherburn, R E; Jenkins, R O

2005-01-01

To investigate aerial release of bacteria from used cot mattresses and to assess factors that may influence this process. Movement on used mattresses, simulating that of an infant's head, significantly enhanced aerial release of naturally acquired bacteria from the polyurethane foams (total count data, P = 0.008; Staphylococcus aureus, P = 0.004) or from polyvinyl chloride covers (total count data, P = 0.001). Aerial release of naturally acquired bacteria from used cot mattresses showed high variability and was poorly correlated (R2 < or = 0.294) with bacterial cell density within the materials. In experiments involving inoculation of S. aureus and Escherichia coli onto the polyurethane of unused cot mattresses, aerial release of the species correlated well (R2 > or = 0.950) with inoculation density when simulated infant head movement was applied. Aerial release of these bacterial species from the material decreased with increase in width or aqueous content of the material, and was lower from polyurethane foam of a used cot mattress. Simulated infant movement and mattress related factors influence aerial release of bacteria from cot mattress materials. With simulated infant movement on cot mattress polyurethane foam, levels of airborne bacteria above the material are proportional to bacterial population levels inoculated onto the material. Cot mattresses harbouring relatively high levels of naturally acquired toxigenic bacteria, such as S. aureus, could pose a relatively high risk of infection to the infant's respiratory tract through increased aerial contamination. This has impact in the context of recent findings on cot mattress related risk factors for sudden infant death syndrome.

DYNER: A DYNamic ClustER for Education and Research

ERIC Educational Resources Information Center

Kehagias, Dimitris; Grivas, Michael; Mamalis, Basilis; Pantziou, Grammati

2006-01-01

Purpose: The purpose of this paper is to evaluate the use of a non-expensive dynamic computing resource, consisting of a Beowulf class cluster and a NoW, as an educational and research infrastructure. Design/methodology/approach: Clusters, built using commodity-off-the-shelf (COTS) hardware components and free, or commonly used, software, provide…

COTS/CRS: KSC Evolving Host Initiatives with Commercial Space Partners

NASA Technical Reports Server (NTRS)

Yohpe, Megan

2010-01-01

NASA's Commercial Crew and Cargo Program Office (C3PO) leads the agency's commercial efforts to stimulate United States private companies as the shuttle program comes to a close. Through the Commercial Orbital Transportation Services (COTS) program, two companies, SpaceX and Orbital, were selected to demonstrate their ability to perform flights to the International Space Station. The Commercial Resupply Services (CRS) Project leverages off the COTS experience, and awarded these two private companies contracts to resupply the International Space Station after shuttle fly out. As a 2010 summer intern, I supported the COTS/CRS team in their team meetings, attended and contributed to project discussions and planning, and assisted in developing visual representations for the variety of processes and organizational endeavors required for the program to run smoothly. One aspect of the COTS/CRS program gives the involved private companies the opportunity to request available services from Kennedy Space Center (KSC); one of my projects included assisting in the development of a related Task Order Request (TOR) process. In addition, an integral part of the project was to maintain and enhance the team database for processing the variety of TORS. My experience in the project gave me great insight into the growing field of commercial space activities. The development of the TOR process involved coordinating representatives from a variety of backgrounds at KSC. A clear and concise visual representation of the TOR process in the form of a flow chart was necessary to successfully implement a task order request from one of NASA's commercial partners. The goals of the process charts were to communicate the team's ideas and foster a common thought process while at the same time allow the process to grow and evolve. It was critical that the requests from the private companies were addressed quickly and thoroughly as the process developed this summer is expected to have extensive future use.

Engineering Documentation and Data Control

NASA Technical Reports Server (NTRS)

Matteson, Michael J.; Bramley, Craig; Ciaruffoli, Veronica

2001-01-01

Mississippi Space Services (MSS) the facility services contractor for NASA's John C. Stennis Space Center (SSC), is utilizing technology to improve engineering documentation and data control. Two identified improvement areas, labor intensive documentation research and outdated drafting standards, were targeted as top priority. MSS selected AutoManager(R) WorkFlow from Cyco software to manage engineering documentation. The software is currently installed on over 150 desctops. The outdated SSC drafting standard was written for pre-CADD drafting methods, in other words, board drafting. Implementation of COTS software solutions to manage engineering documentation and update the drafting standard resulted in significant increases in productivity by reducing the time spent searching for documents.

A Spore Coat Protein, CotS, of Bacillus subtilis Is Synthesized under the Regulation of ςK and GerE during Development and Is Located in the Inner Coat Layer of Spores

PubMed Central

Takamatsu, Hiromu; Chikahiro, Yukari; Kodama, Takeko; Koide, Hidekatsu; Kozuka, Satoshi; Tochikubo, Kunio; Watabe, Kazuhito

1998-01-01

The spore coat of Bacillus subtilis has a unique morphology and consists of polypeptides of different sizes, whose synthesis and assembly are precisely regulated by a cascade of transcription factors and regulatory proteins. We examined the factors that regulate cotS gene expression and CotS assembly into the coat layer of B. subtilis by Northern blot and Western blot analysis. Transcription of cotS mRNA was not detected in sporulating cells of ςK and gerE mutants by Northern blot analysis. By Western blot analysis using anti-CotS antibody, CotS was first detected in protein samples solubilized from wild-type cells at 5 h after the start of sporulation. CotS was not detected in the vegetative cells and spores of a gerE mutant or in the spores of mutants deficient in ςE, ςF, ςG, or ςK. CotS was detected in the sporangium but not in the spores of a cotE mutant. The sequence of the promoter region of cotS was similar to the consensus sequences for binding of ςK and GerE. These results demonstrate that ςK and GerE are required for cotS expression and that CotE is essential for the assembly of CotS in the coat. Immunoelectron microscopic observation using anti-CotS antibody revealed that CotS is located within the spore coat, in particular in the inner coats of dormant spores. PMID:9603889

Identification of a novel human kinase supporter of Ras (hKSR-2) that functions as a negative regulator of Cot (Tpl2) signaling.

PubMed

Channavajhala, Padma L; Wu, Leeying; Cuozzo, John W; Hall, J Perry; Liu, Wei; Lin, Lih-Ling; Zhang, Yuhua

2003-11-21

Kinase suppressor of Ras (KSR) is an integral and conserved component of the Ras signaling pathway. Although KSR is a positive regulator of the Ras/mitogen-activated protein (MAP) kinase pathway, the role of KSR in Cot-mediated MAPK activation has not been identified. The serine/threonine kinase Cot (also known as Tpl2) is a member of the MAP kinase kinase kinase (MAP3K) family that is known to regulate oncogenic and inflammatory pathways; however, the mechanism(s) of its regulation are not precisely known. In this report, we identify an 830-amino acid novel human KSR, designated hKSR-2, using predictions from genomic data base mining based on the structural profile of the KSR kinase domain. We show that, similar to the known human KSR, hKSR-2 co-immunoprecipitates with many signaling components of the Ras/MAPK pathway, including Ras, Raf, MEK-1, and ERK-1/2. In addition, we demonstrate that hKSR-2 co-immunoprecipitates with Cot and that co-expression of hKSR-2 with Cot significantly reduces Cot-mediated MAPK and NF-kappaB activation. This inhibition is specific to Cot, because Ras-induced ERK and IkappaB kinase-induced NF-kappaB activation are not significantly affected by hKSR-2 co-expression. Moreover, Cot-induced interleukin-8 production in HeLa cells is almost completely inhibited by the concurrent expression of hKSR-2, whereas transforming growth factor beta-activated kinase 1 (TAK1)/TAK1-binding protein 1 (TAB1)-induced interleukin-8 production is not affected by hKSR-2 co-expression. Taken together, these results indicate that hKSR-2, a new member of the KSR family, negatively regulates Cot-mediated MAP kinase and NF-kappaB pathway signaling.

Guest Editor's Introduction: Special section on dependable distributed systems

NASA Astrophysics Data System (ADS)

Fetzer, Christof

1999-09-01

We rely more and more on computers. For example, the Internet reshapes the way we do business. A `computer outage' can cost a company a substantial amount of money. Not only with respect to the business lost during an outage, but also with respect to the negative publicity the company receives. This is especially true for Internet companies. After recent computer outages of Internet companies, we have seen a drastic fall of the shares of the affected companies. There are multiple causes for computer outages. Although computer hardware becomes more reliable, hardware related outages remain an important issue. For example, some of the recent computer outages of companies were caused by failed memory and system boards, and even by crashed disks - a failure type which can easily be masked using disk mirroring. Transient hardware failures might also look like software failures and, hence, might be incorrectly classified as such. However, many outages are software related. Faulty system software, middleware, and application software can crash a system. Dependable computing systems are systems we can rely on. Dependable systems are, by definition, reliable, available, safe and secure [3]. This special section focuses on issues related to dependable distributed systems. Distributed systems have the potential to be more dependable than a single computer because the probability that all computers in a distributed system fail is smaller than the probability that a single computer fails. However, if a distributed system is not built well, it is potentially less dependable than a single computer since the probability that at least one computer in a distributed system fails is higher than the probability that one computer fails. For example, if the crash of any computer in a distributed system can bring the complete system to a halt, the system is less dependable than a single-computer system. Building dependable distributed systems is an extremely difficult task. There is no silver bullet solution. Instead one has to apply a variety of engineering techniques [2]: fault-avoidance (minimize the occurrence of faults, e.g. by using a proper design process), fault-removal (remove faults before they occur, e.g. by testing), fault-evasion (predict faults by monitoring and reconfigure the system before failures occur), and fault-tolerance (mask and/or contain failures). Building a system from scratch is an expensive and time consuming effort. To reduce the cost of building dependable distributed systems, one would choose to use commercial off-the-shelf (COTS) components whenever possible. The usage of COTS components has several potential advantages beyond minimizing costs. For example, through the widespread usage of a COTS component, design failures might be detected and fixed before the component is used in a dependable system. Custom-designed components have to mature without the widespread in-field testing of COTS components. COTS components have various potential disadvantages when used in dependable systems. For example, minimizing the time to market might lead to the release of components with inherent design faults (e.g. use of `shortcuts' that only work most of the time). In addition, the components might be more complex than needed and, hence, potentially have more design faults than simpler components. However, given economic constraints and the ability to cope with some of the problems using fault-evasion and fault-tolerance, only for a small percentage of systems can one justify not using COTS components. Distributed systems built from current COTS components are asynchronous systems in the sense that there exists no a priori known bound on the transmission delay of messages or the execution time of processes. When designing a distributed algorithm, one would like to make sure (e.g. by testing or verification) that it is correct, i.e. satisfies its specification. Many distributed algorithms make use of consensus (eventually all non-crashed processes have to agree on a value), leader election (a crashed leader is eventually replaced by a new leader, but at any time there is at most one leader) or a group membership detection service (a crashed process is eventually suspected to have crashed but only crashed processes are suspected). From a theoretical point of view, the service specifications given for such services are not implementable in asynchronous systems. In particular, for each implementation one can derive a counter example in which the service violates its specification. From a practical point of view, the consensus, the leader election, and the membership detection problem are solvable in asynchronous distributed systems. In this special section, Raynal and Tronel show how to bridge this difference by showing how to implement the group membership detection problem with a negligible probability [1] to fail in an asynchronous system. The group membership detection problem is specified by a liveness condition (L) and a safety property (S): (L) if a process p crashes, then eventually every non-crashed process q has to suspect that p has crashed; and (S) if a process q suspects p, then p has indeed crashed. One can show that either (L) or (S) is implementable, but one cannot implement both (L) and (S) at the same time in an asynchronous system. In practice, one only needs to implement (L) and (S) such that the probability that (L) or (S) is violated becomes negligible. Raynal and Tronel propose and analyse a protocol that implements (L) with certainty and that can be tuned such that the probability that (S) is violated becomes negligible. Designing and implementing distributed fault-tolerant protocols for asynchronous systems is a difficult but not an impossible task. A fault-tolerant protocol has to detect and mask certain failure classes, e.g. crash failures and message omission failures. There is a trade-off between the performance of a fault-tolerant protocol and the failure classes the protocol can tolerate. One wants to tolerate as many failure classes as needed to satisfy the stochastic requirements of the protocol [1] while still maintaining a sufficient performance. Since clients of a protocol have different requirements with respect to the performance/fault-tolerance trade-off, one would like to be able to customize protocols such that one can select an appropriate performance/fault-tolerance trade-off. In this special section Hiltunen et al describe how one can compose protocols from micro-protocols in their Cactus system. They show how a group RPC system can be tailored to the needs of a client. In particular, they show how considering additional failure classes affects the performance of a group RPC system. References [1] Cristian F 1991 Understanding fault-tolerant distributed systems Communications of ACM 34 (2) 56-78 [2] Heimerdinger W L and Weinstock C B 1992 A conceptual framework for system fault tolerance Technical Report 92-TR-33, CMU/SEI [3] Laprie J C (ed) 1992 Dependability: Basic Concepts and Terminology (Vienna: Springer)

The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease.

PubMed

Tang, Zeli; Shang, Mei; Chen, Tingjin; Ren, Pengli; Sun, Hengchang; Qu, Hongling; Lin, Zhipeng; Zhou, Lina; Yu, Jinyun; Jiang, Hongye; Zhou, Xinyi; Li, Xuerong; Huang, Yan; Xu, Jin; Yu, Xinbing

2016-12-19

Clonorchiasis, a food-borne zoonosis, is caused by Clonorchis sinensis. The intestinal tract and bile ducts are crucial places for C. sinensis metacercariae to develop into adult worms. The endospore of Bacillus subtilis is an ideal oral immunization vehicle for delivery of heterologous antigens to intestine. Cysteine protease of C. sinensis (CsCP) is an endogenous key component in the excystment of metacercariae and other physiological or pathological processes. We constructed a fusion gene of CotC (a coat protein)-CsCP and obtained B. subtilis spores with recombinant plasmid of pEB03-CotC-CsCP (B.s-CotC-CsCP). CotC-CsCP expressed on spores' surface was detected by Western blotting and immunofluorescence. Immunological characteristics of recombinant spore coat protein were evaluated in a mouse model. The levels of CsCP-specific antibodies were detected by ELISA. Effects of recombinant spores on mouse intestine were evaluated by histological staining. The activities of biochemical enzymes in serum were assayed by microplate. Liver sections of infected mice were evaluated by Ishak score after Masson's trichrome. The B.s-CotC-CsCP spores displayed CsCP on their coat. Specific IgG and isotypes were significantly induced by coat proteins of B.s-CotC-CsCP spores after subcutaneous immunization. IgA levels in intestinal mucus and bile of B.s-CotC-CsCP orally treated mice significantly increased. Additionally, more IgA-secreting cells were observed in enteraden and lamina propria regions of the mouse jejunum, and an increased amount of acidic mucins in intestines were also observed. There were no significant differences in enzyme levels of serum among groups. No inflammatory injury was observed in the intestinal tissues of each group. The degree of liver fibrosis was significantly reduced after oral immunization with B.s-CotC-CsCP spores. Bacillus subtilis spores maintained the original excellent immunogenicity of CsCP expressed on their surface. Both local and systemic specific immune responses were elicited by oral administration of B.s-CotC-CsCP spores. The spores effectively promoted intestinal health by inducing secretion of acidic mucins, with no other side effects to the liver or intestine. Oral administration of spores expressing CsCP could provide effective protection against C. sinensis. This study may be a cornerstone for development of antiparasitic agents or vaccines against clonorchiasis based on B. subtilis spore expressing CsCP on the surface.

The Context Oriented Training Method.

ERIC Educational Resources Information Center

Cavrini, Andrea

The Context Oriented Training (COT) method is introduced and explored in this paper. COT is a means of improving the training process, beginning with the observation and analysis of current corporate experiences in the field. The learning context lies between the development of professional competencies in training and the operational side in the…

Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores.

PubMed

Hinc, Krzysztof; Isticato, Rachele; Dembek, Marcin; Karczewska, Joanna; Iwanicki, Adam; Peszyńska-Sularz, Grazyna; De Felice, Maurilio; Obuchowski, Michał; Ricca, Ezio

2010-01-18

The bacterial endospore (spore) has recently been proposed as a new surface display system. Antigens and enzymes have been successfully exposed on the surface layers of the Bacillus subtilis spore, but only in a few cases the efficiency of expression and the effective surface display and have been determined. We used this heterologous expression system to produce the A subunit of the urease of the animal pathogen Helicobater acinonychis. Ureases are multi-subunit enzymes with a central role in the virulence of various bacterial pathogens and necessary for colonization of the gastric mucosa by the human pathogen H. pylori. The urease subunit UreA has been recognized as a major antigen, able to induce high levels of protection against challenge infections. We expressed UreA from H. acinonychis on the B. subtilis spore coat by using three different spore coat proteins as carriers and compared the efficiency of surface expression and surface display obtained with the three carriers. A combination of western-, dot-blot and immunofluorescence microscopy allowed us to conclude that, when fused to CotB, UreA is displayed on the spore surface (ca. 1 x 10(3) recombinant molecules per spore), whereas when fused to CotC, although most efficiently expressed (7-15 x 10(3) recombinant molecules per spore) and located in the coat layer, it is not displayed on the surface. Experiments with CotG gave results similar to those with CotC, but the CotG-UreA recombinant protein appeared to be partially processed. UreA was efficiently expressed on the spore coat of B. subtilis when fused to CotB, CotC or CotG. Of these three coat proteins CotC allows the highest efficiency of expression, whereas CotB is the most appropriate for the display of heterologous proteins on the spore surface.

Software defined radio (SDR) architecture for concurrent multi-satellite communications

NASA Astrophysics Data System (ADS)

Maheshwarappa, Mamatha R.

SDRs have emerged as a viable approach for space communications over the last decade by delivering low-cost hardware and flexible software solutions. The flexibility introduced by the SDR concept not only allows the realisation of concurrent multiple standards on one platform, but also promises to ease the implementation of one communication standard on differing SDR platforms by signal porting. This technology would facilitate implementing reconfigurable nodes for parallel satellite reception in Mobile/Deployable Ground Segments and Distributed Satellite Systems (DSS) for amateur radio/university satellite operations. This work outlines the recent advances in embedded technologies that can enable new communication architectures for concurrent multi-satellite or satellite-to-ground missions where multi-link challenges are associated. This research proposes a novel concept to run advanced parallelised SDR back-end technologies in a Commercial-Off-The-Shelf (COTS) embedded system that can support multi-signal processing for multi-satellite scenarios simultaneously. The initial SDR implementation could support only one receiver chain due to system saturation. However, the design was optimised to facilitate multiple signals within the limited resources available on an embedded system at any given time. This was achieved by providing a VHDL solution to the existing Python and C/C++ programming languages along with parallelisation so as to accelerate performance whilst maintaining the flexibility. The improvement in the performance was validated at every stage through profiling. Various cases of concurrent multiple signals with different standards such as frequency (with Doppler effect) and symbol rates were simulated in order to validate the novel architecture proposed in this research. Also, the architecture allows the system to be reconfigurable by providing the opportunity to change the communication standards in soft real-time. The chosen COTS solution provides a generic software methodology for both ground and space applications that will remain unaltered despite new evolutions in hardware, and supports concurrent multi-standard, multi-channel and multi-rate telemetry signals.

Refined identification of Vibrio bacterial flora from Acanthasther planci based on biochemical profiling and analysis of housekeeping genes.

PubMed

Rivera-Posada, J A; Pratchett, M; Cano-Gomez, A; Arango-Gomez, J D; Owens, L

2011-09-09

We used a polyphasic approach for precise identification of bacterial flora (Vibrionaceae) isolated from crown-of-thorns starfish (COTS) from Lizard Island (Great Barrier Reef, Australia) and Guam (U.S.A., Western Pacific Ocean). Previous 16S rRNA gene phylogenetic analysis was useful to allocate and identify isolates within the Photobacterium, Splendidus and Harveyi clades but failed in the identification of Vibrio harveyi-like isolates. Species of the V harveyi group have almost indistinguishable phenotypes and genotypes, and thus, identification by standard biochemical tests and 16S rRNA gene analysis is commonly inaccurate. Biochemical profiling and sequence analysis of additional topA and mreB housekeeping genes were carried out for definitive identification of 19 bacterial isolates recovered from sick and wild COTS. For 8 isolates, biochemical profiles and topA and mreB gene sequence alignments with the closest relatives (GenBank) confirmed previous 16S rRNA-based identification: V. fortis and Photobacterium eurosenbergii species (from wild COTS), and V natriegens (from diseased COTS). Further phylogenetic analysis based on topA and mreB concatenated sequences served to identify the remaining 11 V harveyi-like isolates: V. owensii and V. rotiferianus (from wild COTS), and V. owensii, V. rotiferianus, and V. harveyi (from diseased COTS). This study further confirms the reliability of topA-mreB gene sequence analysis for identification of these close species, and it reveals a wider distribution range of the potentially pathogenic V. harveyi group.

Performance Evaluation of Cots Uav for Architectural Heritage Documentation. a Test on S.GIULIANO Chapel in Savigliano (cn) - Italy

NASA Astrophysics Data System (ADS)

Chiabrando, F.; Teppati Losè, L.

2017-08-01

Even more the use of UAV platforms is a standard for images or videos acquisitions from an aerial point of view. According to the enormous growth of requests, we are assisting to an increasing of the production of COTS (Commercial off the Shelf) platforms and systems to answer to the market requirements. In this last years, different platforms have been developed and sell at low-medium cost and nowadays the offer of interesting systems is very large. One of the most important company that produce UAV and other imaging systems is the DJI (Dà-Jiāng Innovations Science and Technology Co., Ltd) founded in 2006 headquartered in Shenzhen - China. The platforms realized by the company range from low cost systems up to professional equipment, tailored for high resolution acquisitions useful for film maker purposes. According to the characteristics of the last developed low cost DJI platforms, the onboard sensors and the performance of the modern photogrammetric software based on Structure from Motion (SfM) algorithms, those systems are nowadays employed for performing 3D surveys starting from the small up to the large scale. The present paper is aimed to test the characteristic in terms of image quality, flight operations, flight planning and accuracy evaluation of the final products of three COTS platforms realized by DJI: the Mavic Pro, the Phantom 4 and the Phantom 4 PRO. The test site chosen was the Chapel of San Giuliano in the municipality of Savigliano (Cuneo-Italy), a small church with two aisles dating back to the early eleventh century.

Simple-1: Development stage of the data transmission system for a solid propellant mid-power rocket model

NASA Astrophysics Data System (ADS)

Yarce, Andrés; Sebastián Rodríguez, Juan; Galvez, Julián; Gómez, Alejandro; García, Manuel J.

2017-06-01

This paper presents the development stage of a communication module for a solid propellant mid-power rocket model. The communication module was named. Simple-1 and this work considers its design, construction and testing. A rocket model Estes Ventris Series Pro II® was modified to introduce, on the top of the payload, several sensors in a CanSat form factor. The Printed Circuit Board (PCB) was designed and fabricated from Commercial Off The Shelf (COTS) components and assembled in a cylindrical rack structure similar to this small format satellite concept. The sensors data was processed using one Arduino Mini and transmitted using a radio module to a Software Defined Radio (SDR) HackRF based platform on the ground station. The Simple-1 was tested using a drone in successive releases, reaching altitudes from 200 to 300 meters. Different kind of data, in terms of altitude, position, atmospheric pressure and vehicle temperature were successfully measured, making possible the progress to a next stage of launching and analysis.

Cot kinase plays a critical role in Helicobacter pylori-induced IL-8 expression.

PubMed

Jang, Sungil; Kim, Jinmoon; Cha, Jeong-Heon

2017-04-01

Helicobacter pylori is a major pathogen causing various gastric diseases including gastric cancer. Infection of H. pylori induces pro-inflammatory cytokine IL-8 expression in gastric epithelial cells in the initial inflammatory process. It has been known that H. pylori can modulate Ras-Raf-Mek-Erk signal pathway for IL-8 induction. Recently, it has been shown that another signal molecule, cancer Osaka thyroid oncogene/tumor progression locus 2 (Cot/Tpl2) kinase, activates Mek and Erk and plays a role in the Erk pathway, similar to MAP3K signal molecule Raf kinase. Therefore, the objective of this study was to determine whether Cot kinase might be involved in IL-8 induction caused by H. pylori infection. AGS gastric epithelial cells were infected by H. pylori strain G27 or its isogenic mutants lacking cagA or type IV secretion system followed by treatment with Cot kinase inhibitor (KI) or siRNA specific for Cot kinase. Activation of Erk was assessed by Western blot analysis and expression of IL-8 was measured by ELISA. Treatment with Cot KI reduced both transient and sustained Erk activation. It also reduced early and late IL-8 secretion in the gastric epithelial cell line. Furthermore, siRNA knockdown of Cot inhibited early and late IL-8 secretion induced by H. pylori infection. Taken together, these results suggest that Cot kinase might play a critical role in H. pylori type IV secretion apparatus-dependent early IL-8 secretion and CagA-dependent late IL-8 secretion as an alternative signaling molecule in the Erk pathway.

The Sunset Supply Base: Long Term COTS Supportability, Implementing Affordable Methods and Processes

DTIC Science & Technology

2003-03-01

7 D. BENEFITS OF THE SSB SYSTEM...69 Table 11: Summary of SSB Financial and Non-Financial Benefits ................................. 73 Table 12: Alignment with...SSB Specific Goals to Derived Benefits ................................ 74 xi THIS PAGE INTENTIONALLY LEFT BLANK xii LIST OF ABBREVIATIONS AND

Manned/Unmanned Common Architecture Program (MCAP) net centric flight tests

NASA Astrophysics Data System (ADS)

Johnson, Dale

2009-04-01

Properly architected avionics systems can reduce the costs of periodic functional improvements, maintenance, and obsolescence. With this in mind, the U.S. Army Aviation Applied Technology Directorate (AATD) initiated the Manned/Unmanned Common Architecture Program (MCAP) in 2003 to develop an affordable, high-performance embedded mission processing architecture for potential application to multiple aviation platforms. MCAP analyzed Army helicopter and unmanned air vehicle (UAV) missions, identified supporting subsystems, surveyed advanced hardware and software technologies, and defined computational infrastructure technical requirements. The project selected a set of modular open systems standards and market-driven commercial-off-theshelf (COTS) electronics and software, and, developed experimental mission processors, network architectures, and software infrastructures supporting the integration of new capabilities, interoperability, and life cycle cost reductions. MCAP integrated the new mission processing architecture into an AH-64D Apache Longbow and participated in Future Combat Systems (FCS) network-centric operations field experiments in 2006 and 2007 at White Sands Missile Range (WSMR), New Mexico and at the Nevada Test and Training Range (NTTR) in 2008. The MCAP Apache also participated in PM C4ISR On-the-Move (OTM) Capstone Experiments 2007 (E07) and 2008 (E08) at Ft. Dix, NJ and conducted Mesa, Arizona local area flight tests in December 2005, February 2006, and June 2008.

Use of COTS in the Multimission Advanced Ground Intelligent Control (MAGIC) program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crowley, N.L.

1997-11-01

This tutorial will discuss the experiences of the Space System Technologies Division of the USAF Phillips Laboratory (PL/VTS) in developing a COTS-based satellite control system. The system`s primary use is a testbed for new technologies that are intended for future integration into the operational satellite control system. As such, the control system architecture must be extremely open and flexible so we can integrate new components and functions easily and also provide our system to contractors for their component work. The system is based on commercial hardware, is based on Windows NT, and makes the maximum use of COTS components andmore » industry standards.« less

The Design of a Fault-Tolerant COTS-Based Bus Architecture for Space Applications

NASA Technical Reports Server (NTRS)

Chau, Savio N.; Alkalai, Leon; Tai, Ann T.

2000-01-01

The high-performance, scalability and miniaturization requirements together with the power, mass and cost constraints mandate the use of commercial-off-the-shelf (COTS) components and standards in the X2000 avionics system architecture for deep-space missions. In this paper, we report our experiences and findings on the design of an IEEE 1394 compliant fault-tolerant COTS-based bus architecture. While the COTS standard IEEE 1394 adequately supports power management, high performance and scalability, its topological criteria impose restrictions on fault tolerance realization. To circumvent the difficulties, we derive a "stack-tree" topology that not only complies with the IEEE 1394 standard but also facilitates fault tolerance realization in a spaceborne system with limited dedicated resource redundancies. Moreover, by exploiting pertinent standard features of the 1394 interface which are not purposely designed for fault tolerance, we devise a comprehensive set of fault detection mechanisms to support the fault-tolerant bus architecture.

Development of a COTS Mass Storage Unit for the Space Readiness Coherent Lidar Experiment

NASA Technical Reports Server (NTRS)

Liggin, Karl; Clark, Porter

1999-01-01

The technology to develop a Mass Storage Unit (MSU) using commercial-off-the-shelf (COTS) hard drives is an on-going challenge to meet the Space Readiness Coherent Lidar Experiment (SPARCLE) program requirements. A conceptual view of SPARCLE's laser collecting atmospheric data from the shuttle is shown in Figure 1. The determination to develop this technology required several in depth studies before an actual COTS hard drive was selected to continue this effort. Continuing the development of the MSU can, and will, serve future NASA programs that require larger data storage and more on-board processing.

Transcriptome Analysis of Neonatal Larvae after Hyperthermia-Induced Seizures in the Contractile Silkworm, Bombyx mori

PubMed Central

Nie, Hongyi; Liu, Chun; Zhang, Yinxia; Zhou, Mengting; Huang, Xiaofeng; Peng, Li; Xia, Qingyou

2014-01-01

The ability to respond quickly and efficiently to transient extreme environmental conditions is an important property of all biota. However, the physiological basis of thermotolerance in different species is still unclear. Here, we found that the cot mutant showed a seizure phenotype including contraction of the body, rolling, vomiting gut juice and a momentary cessation of movement, and the heartbeat rhythm of the dorsal vessel significantly increases after hyperthermia. To comprehensively understand this process at the molecular level, the transcriptomic profile of cot mutant, which is a behavior mutant that exhibits a seizure phenotype, was investigated after hyperthermia (42°C) that was induced for 5 min. By digital gene expression profiling, we determined the gene expression profile of three strains (cot/cot ok/ok, +/+ ok/ok and +/+ +/+) under hyperthermia (42°C) and normal (25°C) conditions. A Venn diagram showed that the most common differentially expressed genes (DEGs, FDR<0.01 and log2 Ratio≥1) were up-regulated and annotated with the heat shock proteins (HSPs) in 3 strains after treatment with hyperthermia, suggesting that HSPs rapidly increased in response to high temperature; 110 unique DEGs, could be identified in the cot mutant after inducing hyperthermia when compared to the control strains. Of these 110 unique DEGs, 98.18% (108 genes) were up-regulated and 1.82% (two genes) were down-regulated in the cot mutant. KEGG pathways analysis of these unique DEGs suggested that the top three KEGG pathways were “Biotin metabolism,” “Fatty acid biosynthesis” and “Purine metabolism,” implying that diverse metabolic processes are active in cot mutant induced-hyperthermia. Unique DEGs of interest were mainly involved in the ubiquitin system, nicotinic acetylcholine receptor genes, cardiac excitation–contraction coupling or the Notch signaling pathway. Insights into hyperthermia-induced alterations in gene expression and related pathways could yield hints for understanding the relationship between behaviors and environmental stimuli (hyperthermia) in insects. PMID:25423472

Spectral Dynamics Inc., ships hybrid, 316-channel data acquisition system to Sandia Labs.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schwartz, Douglas

2003-09-01

Spectral Dynamics announced the shipment of a 316-channel data acquisition system. The system was custom designed for the Light Initiated High Explosive (LIHE) facility at Sandia Labs in Albuquerque, New Mexico by Spectral Dynamics Advanced Research Products Group. This Spectral Dynamics data acquisition system was tailored to meet the unique LIHE environmental and testing requirements utilizing Spectral Dynamics commercial off the shelf (COTS) Jaguar and VIDAS products supplemented by SD Alliance partner's (COTS) products. 'This system is just the beginning of our cutting edge merged technology solutions,' stated Mark Remelman, Manager for the Spectral Dynamics Advanced Research Products Group. 'Thismore » Hybrid system has 316-channels of data acquisition capability, comprised of 102.4kHz direct to disk acquisition and 2.5MHz, 200Mhz & 500Mhz RAM based capabilities. In addition it incorporates the advanced bridge conditioning and dynamic configuration capabilities offered by Spectral Dynamics new Smart Interface Panel System (SIPS{trademark}).' After acceptance testing, Tony King, the Instrumentation Engineer facilitating the project for the Sandia LIHE group commented; 'The LIHE staff was very impressed with the design, construction, attention to detail and overall performance of the instrumentation system'. This system combines VIDAS, a leading edge fourth generation SD-VXI hardware and field-proven software system from SD's Advanced Research Products Group with SD's Jaguar, a multiple Acquisition Control Peripheral (ACP) system that allows expansion to hundreds of channels without sacrificing signal processing performance. Jaguar incorporates dedicated throughput disks for each ACP providing time streaming to disk at up to the maximum sample rate. Spectral Dynamics, Inc. is a leading worldwide supplier of systems and software for advanced computer-automated data acquisition, vibration testing, structural dynamics, explosive shock, high-speed transient capture, acoustic analysis, monitoring, measurement, control and backup. Spectral Dynamics products are used for research, design verification, product testing and process improvement by manufacturers of all types of electrical, electronic and mechanical products, as well as by universities and government-funded agencies. The Advanced Research Products Group is the newest addition to the Spectral Dynamics family. Their newest VXI data acquisition hardware pushes the envelope on capabilities and embodies the same rock solid design methodologies, which have always differentiated Spectral Dynamics from its competition.« less

Comparison of Computational and Experimental Results for a Transonic Variable-Speed Power-Turbine Blade Operating with Low Inlet Turbulence Levels

NASA Technical Reports Server (NTRS)

Booth, David; Flegel, Ashlie

2015-01-01

A computational assessment of the aerodynamic performance of the midspan section of a variable-speed power-turbine blade is described. The computation comprises a periodic single blade that represents the 2-D Midspan section VSPT blade that was tested in the NASA Glenn Research Center Transonic Turbine Blade Cascade Facility. A commercial, off-the-shelf (COTS) software package, Pointwise and CFD++, was used for the grid generation and RANS and URANS computations. The CFD code, which offers flexibility in terms of turbulence and transition modeling options, was assessed in terms of blade loading, loss, and turning against test data from the transonic tunnel. Simulations were assessed at positive and negative incidence angles that represent the turbine cruise and take-off design conditions. The results indicate that the secondary flow induced at the positive incidence cruise condition results in a highly loaded case and transitional flow on the blade is observed. The negative incidence take-off condition is unloaded and the flow is very two-dimensional. The computational results demonstrate the predictive capability of the gridding technique and COTS software for a linear transonic turbine blade cascade with large incidence angle variation.

Comparison of Computational and Experimental Results for a Transonic Variable-speed Power-Turbine Blade Operating with Low Inlet Turbulence Levels

NASA Technical Reports Server (NTRS)

Booth, David T.; Flegel, Ashlie B.

2015-01-01

A computational assessment of the aerodynamic performance of the midspan section of a variable-speed power-turbine blade is described. The computation comprises a periodic single blade that represents the 2-D Midspan section VSPT blade that was tested in the NASA Glenn Research Center Transonic Turbine Blade Cascade Facility. A commercial, off-the-shelf (COTS) software package, Pointwise and CFD++, was used for the grid generation and RANS and URANS computations. The CFD code, which offers flexibility in terms of turbulence and transition modeling options, was assessed in terms of blade loading, loss, and turning against test data from the transonic tunnel. Simulations were assessed at positive and negative incidence angles that represent the turbine cruise and take-off design conditions. The results indicate that the secondary flow induced at the positive incidence cruise condition results in a highly loaded case and transitional flow on the blade is observed. The negative incidence take-off condition is unloaded and the flow is very two-dimensional. The computational results demonstrate the predictive capability of the gridding technique and COTS software for a linear transonic turbine blade cascade with large incidence angle variation.

Atomic force microscopy imaging and single molecule recognition force spectroscopy of coat proteins on the surface of Bacillus subtilis spore.

PubMed

Tang, Jilin; Krajcikova, Daniela; Zhu, Rong; Ebner, Andreas; Cutting, Simon; Gruber, Hermann J; Barak, Imrich; Hinterdorfer, Peter

2007-01-01

Coat assembly in Bacillus subtilis serves as a tractable model for the study of the self-assembly process of biological structures and has a significant potential for use in nano-biotechnological applications. In the present study, the morphology of B. subtilis spores was investigated by magnetically driven dynamic force microscopy (MAC mode atomic force microscopy) under physiological conditions. B. subtilis spores appeared as prolate structures, with a length of 0.6-3 microm and a width of about 0.5-2 microm. The spore surface was mainly covered with bump-like structures with diameters ranging from 8 to 70 nm. Besides topographical explorations, single molecule recognition force spectroscopy (SMRFS) was used to characterize the spore coat protein CotA. This protein was specifically recognized by a polyclonal antibody directed against CotA (anti-CotA), the antibody being covalently tethered to the AFM tip via a polyethylene glycol linker. The unbinding force between CotA and anti-CotA was determined as 55 +/- 2 pN. From the high-binding probability of more than 20% in force-distance cycles it is concluded that CotA locates in the outer surface of B. subtilis spores. Copyright (c) 2007 John Wiley & Sons, Ltd.

The development of CotA mediator cocktail system for dyes decolorization.

PubMed

Luo, S; Xie, T; Liu, Z; Sun, F; Wang, G

2018-05-01

The increasing use of dyes leads to serious environmental concerns, it is significant to explore eco-friendly and economic approaches for dye decolorization. This study aimed to develop mediator cocktail (AS and ABTS) for enhancing the capability of laccase-mediator system in the removal of dyes. By mediator screening, the mediators of ABTS and AS (ABTS, 2, 2'-azino-bis-(3-ethylbenzothiazo-thiazoline-6-sulphonic acid); AS, acetosyringone) were combined for dyes decolorization. The Box-Behnken Design and response surface analysis was performed to optimize experiment conditions. Comparing the CotA-ABTS-AS cocktail system with CotA-single mediator system showed that the coupling of ABTS and AS could increase the decolorization rate 15 times higher, save a third of the cost and shorten the reaction time by 50%. In addition, our studies revealed that sequential oxidation may occur in CotA-ABTS-AS system. Compared with CotA laccase-single mediator system, the CotA-ABTS-AS cocktail system showed advantages including higher efficiency, lower cost and shorter reaction time. This was the first report on the dyes decolorization by laccase mediator cocktail system. These results paved the curb for the application of laccase mediator system in various industrial processes. © 2018 The Society for Applied Microbiology.

Differential regulation of p65 and c-Rel NF-kappaB transactivating activity by Cot, protein kinase C zeta and NIK protein kinases in CD3/CD28 activated T cells.

PubMed

Sánchez-Valdepeñas, Carmen; Punzón, Carmen; San-Antonio, Belén; Martin, Angel G; Fresno, Manuel

2007-03-01

It has been shown that phosphorylation of p65/RelA and c-Rel plays a role in the regulation of transcriptional activity of NF-kappaB independent on IkappaB degradation. In this study, we show that anti CD3/CD28 activation induces the transactivation activity of both p65/RelA and c-Rel in T cells using Gal4 dependent assays. Moreover, protein kinase C (PKC)zeta, Cot kinase and NF-kappaB-inducing kinase (NIK) seem to be involved in those processes in a different manner. Thus, transfection of dominant negative forms of Cot and PKCzeta inhibits CD3/CD28 induction of Gal4-p65 transactivation, whereas the kinase inactive versions of the 3 kinases inhibit induction of Gal4-c-Rel. Cot induction of Gal4-c-Rel transactivating activity seems to be mediated sequentially through PKCzeta and NIK activation, since dominant negative form of NIK blocks Cot and PKCzeta induction, whereas kinase inactive PKCzeta only blocks Cot activity. In contrast, the contribution of NIK to the transactivation function of p65/RelA seems to be negligible and more importantly NIK-KD did not inhibit induction by Cot and PKCzeta. Besides, the enhancing effect of Cot on Gal4-p65 was not decreased in mouse embryo fibroblasts from NIK deficient aly/aly mice in contrast with a greatest reduction on Gal4-c-Rel. By using Ser to Ala mutants in p65 and c-Rel transactivation domains, PKCzeta and NIK activities seem to be dependent of a restricted set of Ser in both proteins. In contrast, the enhancing effect of Cot seems to be less dependent of a particular set of Ser residues being partially abrogated by mutation of several Ser residues.

Rifting-to-drifting transition of the South China Sea: Moho reflection characteristics in continental-ocean transition zone

NASA Astrophysics Data System (ADS)

Wen, Y.; Li, C.

2017-12-01

Dispute remains on the process of continental rifting to subsequent seafloor spreading in the South China Sea (SCS). Several crust-scale multi-channel seismic reflection profiles acquired in the continent-ocean transition zone (COT) of the SCS provide a detailed overview of Moho and deep crustal reflectors and give key information on rifting-to-drifting transition of the area. Moho has strong but discontinuous seismic reflection in COT. These discontinuities are mainly located in the landward side of continent-ocean boundary (COB), and may own to upwelling of lower crustal materials during initial continental extension, leading to numerous volcanic edifices and volcanic ridges. The continental crust in COT shows discontinuous Moho reflections at 11-8.5 s in two-way travel time (twtt), and thins from 18-20.5 km under the uppermost slope to 6-7 km under the lower slope, assuming an average crustal velocity of 6.0 km/s. The oceanic crust has Moho reflections of moderate to high continuity mostly at 1.8-2.2 s twtt below the top of the igneous basement, which means that the crustal thickness excluding sediment layer in COT is 5.4-6.6 km. Subhorizontal Moho reflections are often abruptly interrupted by large seaward dipping normal faults in southern COT but are more continuous compared with the fluctuant and very discontinuous Moho reflections in northern COT. The thickness of thinned continental crust (4.2-4.8 km) is smaller than that of oceanic crust (5.4-6.0 km) near southern COB, indicating that the continental crust has experienced a long period of rifting before seafloor spreading started. The smaller width of northern COT (0-40 km) than in southern COT (0-60 km), and thinner continental crust in southern COT, all indicate that the continental margin rifting and extension was asymmetric. The COT width in the SCS is narrower than that found in other magma-poor continental margins, indicating a swift transition from the final stage of rifting to the inception of normal seafloor spreading.

Control software and electronics architecture design in the framework of the E-ELT instrumentation

NASA Astrophysics Data System (ADS)

Di Marcantonio, P.; Coretti, I.; Cirami, R.; Comari, M.; Santin, P.; Pucillo, M.

2010-07-01

During the last years the European Southern Observatory (ESO), in collaboration with other European astronomical institutes, has started several feasibility studies for the E-ELT (European-Extremely Large Telescope) instrumentation and post-focal adaptive optics. The goal is to create a flexible suite of instruments to deal with the wide variety of scientific questions astronomers would like to see solved in the coming decades. In this framework INAF-Astronomical Observatory of Trieste (INAF-AOTs) is currently responsible of carrying out the analysis and the preliminary study of the architecture of the electronics and control software of three instruments: CODEX (control software and electronics) and OPTIMOS-EVE/OPTIMOS-DIORAMAS (control software). To cope with the increased complexity and new requirements for stability, precision, real-time latency and communications among sub-systems imposed by these instruments, new solutions have been investigated by our group. In this paper we present the proposed software and electronics architecture based on a distributed common framework centered on the Component/Container model that uses OPC Unified Architecture as a standard layer to communicate with COTS components of three different vendors. We describe three working prototypes that have been set-up in our laboratory and discuss their performances, integration complexity and ease of deployment.

Crystal structure of CotA laccase complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) at a novel binding site

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Zhongchuan; Xie, Tian; Key Laboratory of Environmental Microbiology of Sichuan Province, Chengdu 610041, People’s Republic of

2016-03-24

The crystal structure of CotA complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) in a hole motif has been solved; this novel binding site could be a potential structure-based target for protein engineering of CotA laccase. The CotA laccase from Bacillus subtilis is an abundant component of the spore outer coat and has been characterized as a typical laccase. The crystal structure of CotA complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) in a hole motif has been solved. The novel binding site was about 26 Å away from the T1 binding pocket. Comparison with known structures of other laccases revealed that the hole is a specific feature ofmore » CotA. The key residues Arg476 and Ser360 were directly bound to ABTS. Site-directed mutagenesis studies revealed that the residues Arg146, Arg429 and Arg476, which are located at the bottom of the novel binding site, are essential for the oxidation of ABTS and syringaldazine. Specially, a Thr480Phe variant was identified to be almost 3.5 times more specific for ABTS than for syringaldazine compared with the wild type. These results suggest this novel binding site for ABTS could be a potential target for protein engineering of CotA laccases.« less

Canes Implementation: Analysis of Budgetary, Business, and Policy Challenges

DTIC Science & Technology

2014-12-01

Concept of Operations COTS Commercial Off the Shelf DAG Defense Acquisitions Guidebook DAS Defense Acquisitions System DOD Department of Defense...and Integration NSS National Security Strategy OA Open Architecture OCO Overseas Contingency Operations OEF Operation Enduring Freedom OIF...and software and civilian-type open architecture ( OA ) presents a series of challenges. The purpose of this report is to provide the Navy with a

System Testing of Ground Cooling System Components

NASA Technical Reports Server (NTRS)

Ensey, Tyler Steven

2014-01-01

This internship focused primarily upon software unit testing of Ground Cooling System (GCS) components, one of the three types of tests (unit, integrated, and COTS/regression) utilized in software verification. Unit tests are used to test the software of necessary components before it is implemented into the hardware. A unit test determines that the control data, usage procedures, and operating procedures of a particular component are tested to determine if the program is fit for use. Three different files are used to make and complete an efficient unit test. These files include the following: Model Test file (.mdl), Simulink SystemTest (.test), and autotest (.m). The Model Test file includes the component that is being tested with the appropriate Discrete Physical Interface (DPI) for testing. The Simulink SystemTest is a program used to test all of the requirements of the component. The autotest tests that the component passes Model Advisor and System Testing, and puts the results into proper files. Once unit testing is completed on the GCS components they can then be implemented into the GCS Schematic and the software of the GCS model as a whole can be tested using integrated testing. Unit testing is a critical part of software verification; it allows for the testing of more basic components before a model of higher fidelity is tested, making the process of testing flow in an orderly manner.

The Global Influence of Cloud Optical Thickness on Terrestrial Carbon Uptake

NASA Astrophysics Data System (ADS)

Zhu, P.; Cheng, S. J.; Keppel-Aleks, G.; Butterfield, Z.; Steiner, A. L.

2016-12-01

Clouds play a critical role in regulating Earth's climate. One important way is by changing the type and intensity of solar radiation reaching the Earth's surface, which impacts plant photosynthesis. Specifically, the presence of clouds modifies photosynthesis rates by influencing the amount of diffuse radiation as well as the spectral distribution of solar radiation. Satellite-derived cloud optical thickness (COT) may provide the observational constraint necessary to assess the role of clouds on ecosystems and terrestrial carbon uptake across the globe. Previous studies using ground-based observations at individual sites suggest that below a COT of 7, there is a greater increase in light use efficiency than at higher COT values, providing evidence for higher carbon uptake rates than expected given the reduction in radiation by clouds. However, the strength of the COT-terrestrial carbon uptake correlation across the globe remains unknown. In this study, we investigate the influence of COT on terrestrial carbon uptake on a global scale, which may provide insights into cloud conditions favorable for plant photosynthesis and improve our estimates of the land carbon sink. Global satellite-derived MODIS data show that tropical and subtropical regions tend to have COT values around or below the threshold during growing seasons. We find weak correlations between COT and GPP with Fluxnet MTE global GPP data, which may be due to the uncertainty of upscaling GPP from individual site measurements. Analysis with solar-induced fluorescence (SIF) as a proxy for GPP is also evaluated. Overall, this work constructs a global picture of the role of COT on terrestrial carbon uptake, including its temporal and spatial variations.

Cerocene Revisited: The Electronic Structure of and Interconversion Between Ce2(C8H8)3 and Ce(C8H8)2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walter, Marc D.; Booth, Corwin H.; Lukens, Wayne W.

2009-02-02

New synthetic procedures for the preparation of Ce(cot)2, cerocene, from [Li(thf)4][Ce(cot)2], and Ce2(cot)3 in high yield and purity are reported. Heating solid Ce(cot)2 yields Ce2(cot)3 and COT while heating Ce2(cot)3 with an excess of COT in C6D6 to 65oC over four months yields Ce(cot)2. The solid state magnetic susceptibility of these three organocerium compounds shows that Ce(cot)2 behaves as a TIP (temperature independent paramagnet) over the temperature range of 5-300 K, while that of Ce2(cot)3 shows that the spin carriers are antiferromagnetically coupled below 10 K; above 10 K, the individual spins are uncorrelated, and [Ce(cot)2]- behaves as an isolatedmore » f1 paramagnet. The EPR at 1.5K for Ce2(cot)3 and [Ce(cot)2]- have ground state of MJ= +- 1/2. The LIII edge XANES of Ce(cot)2 (Booth, C.H.; Walter, M.D.; Daniel, M.; Lukens, W.W., Andersen, R.A., Phys. Rev. Lett. 2005, 95, 267202) and 2Ce2(cot)3 over 30-500 K are reported; the Ce(cot)2 XANES spectra show Ce(III) and Ce(IV) signatures up to a temperature of approximately 500 K, whereupon the Ce(IV) signature disappears, consistent with the thermal behavior observed in the melting experiment. The EXAFS of Ce(cot)2 and Ce2(cot)3 are reported at 30 K; the agreement between the molecular parameters for Ce(cot)2 derived from EXAFS and single crystal X-ray diffraction data are excellent. In the case of Ce2(cot)3 no X-ray diffraction data are known to exist, but the EXAFS are consistent with a"triple-decker" sandwich structure. A molecular rationalization is presented for the electronic structure of cerocene having a multiconfiguration ground state that is an admixture of the two configurations Ce(III, 4f1)(cot1.5-)2 and Ce(IV, 4f0)(cot2-)2; the multiconfigurational ground state has profound effects on the magnetic properties and on the nature of the chemical bond in cerocene and, perhaps, other molecules.« less

Future manned systems advanced avionics study

NASA Technical Reports Server (NTRS)

Sawamura, Bob; Radke, Kathie

1992-01-01

COTS+ was defined in this study as commercial off-the-shelf (COTS) products, ruggedized and militarized components, and COTS technology. This study cites the benefits of integrating COTS+ in space, postulates a COTS+ integration methodology, and develops requirements and an architecture to achieve integration. Developmental needs and concerns were identified throughout the study; these needs, concerns, and recommendations relative to their abatement are subsequently presented for further action and study. The COTS+ concept appears workable in part or in totality. No COTS+ technology gaps were identified; however, radiation tolerance was cited as a concern, and the deferred maintenance issue resurfaced. Further study is recommended to explore COTS+ cost-effectiveness, maintenance philosophy, needs, concerns, and utility metrics. The generation of a development plan to further investigate and integrate COTS+ technology is recommended. A COTS+ transitional integration program is recommended. Sponsoring and establishing technology maturation programs and COTS+ engineering and standards committees are deemed necessary and are recommended for furthering COTS+ integration in space.

The Evolution of On-Board Emergency Training for the International Space Station Crew

NASA Technical Reports Server (NTRS)

LaBuff, Skyler

2015-01-01

The crew of the International Space Station (ISS) receives extensive ground-training in order to safely and effectively respond to any potential emergency event while on-orbit, but few people realize that their training is not concluded when they launch into space. The evolution of the emergency On- Board Training events (OBTs) has recently moved from paper "scripts" to an intranet-based software simulation that allows for the crew, as well as the flight control teams in Mission Control Centers across the world, to share in an improved and more realistic training event. This emergency OBT simulator ensures that the participants experience the training event as it unfolds, completely unaware of the type, location, or severity of the simulated emergency until the scenario begins. The crew interfaces with the simulation software via iPads that they keep with them as they translate through the ISS modules, receiving prompts and information as they proceed through the response. Personnel in the control centers bring up the simulation via an intranet browser at their console workstations, and can view additional telemetry signatures in simulated ground displays in order to assist the crew and communicate vital information to them as applicable. The Chief Training Officers and emergency instructors set the simulation in motion, choosing the type of emergency (rapid depressurization, fire, or toxic atmosphere) and specific initial conditions to emphasize the desired training objectives. Project development, testing, and implementation was a collaborative effort between ISS emergency instructors, Chief Training Officers, Flight Directors, and the Crew Office using commercial off the shelf (COTS) hardware along with simulation software created in-house. Due to the success of the Emergency OBT simulator, the already-developed software has been leveraged and repurposed to develop a new emulator used during fire response ground-training to deliver data that the crew receives from the handheld Compound Specific Analyzer for Combustion Products (CSA-CP). This CSA-CP emulator makes use of a portion of codebase from the Emergency OBT simulator dealing with atmospheric contamination during fire scenarios, and feeds various data signatures to crew via an iPod Touch with a flight-like CSA-CP display. These innovative simulations, which make use of COTS hardware with custom in-house software, have yielded drastic improvements to emergency training effectiveness and risk reduction for ISS crew and flight control teams during on-orbit and ground training events.

Trauma system evaluation in developing countries: applicability of American College of Surgeons/Committee on Trauma (ACS/COT) basic criteria.

PubMed

Latifi, Rifat; Ziemba, Michelle; Leppäniemi, Ari; Dasho, Erion; Dogjani, Agron; Shatri, Zhaneta; Kociraj, Agim; Oldashi, Fatos; Shosha, Lida

2014-08-01

Trauma continues to be a major health problem worldwide, particularly in the developing world, with high mortality and morbidity. Yet most developing countries lack an organized trauma system. Furthermore, developing countries do not have in place any accreditation process for trauma centers; thus, no accepted standard assessment tools exist to evaluate their trauma services. The aims of this study were to evaluate the trauma system in Albania, using the basic trauma criteria of the American College of Surgeons/Committee on Trauma (ACS/COT) as assessment tools, and to provide the Government with a situational analysis relative to these criteria. We used the ACS/COT basic criteria as assessment tools to evaluate the trauma system in Albania. We conducted a series of semi-structured interviews, unstructured interviews, and focus groups with all stakeholders at the Ministry of Health, at the University Trauma Hospital (UTH) based in Tirana (the capital city), and at ten regional hospitals across the country. Albania has a dedicated national trauma center that serves as the only tertiary center, plus ten regional hospitals that provide some trauma care. However, overall, its trauma system is in need of major reforms involving all essential elements in order to meet the basic requirements of a structured trauma system. The ACS/COT basic criteria can be used as assessment tools to evaluate trauma care in developing countries. Further studies are needed in other developing countries to validate the applicability of these criteria.

A biomechanical and subjective assessment and comparison of three ambulance cot design configurations.

PubMed

Sommerich, Carolyn M; Lavender, Steven A; Radin Umar, Radin Zaid; Le, Peter; Mehta, Jay; Ko, Pei-Ling; Farfan, Rafael; Dutt, Mohini; Park, SangHyun

2012-01-01

Effects of ambulance cot design features (handle design and leg folding mechanism) were evaluated. Experienced ambulance workers performed tasks simulating loading and unloading a cot to and from an ambulance, and a cot raising task. Muscle activity, ratings of perceived exertion, and performance style were significantly affected by cot condition (p < 0.05). Erector Spinae activity was significantly less when using Cot-2's stretcher-style handles. Shoulder muscle activity was significantly less when using Cot-2's loop handle. During loading and unloading, operators allowed the cot to support its own weight most often with Cot-2's stretcher-style handles. Preference for Cot-2 (either handles) over Cot-1 (with loop handle) was consistent across tasks. Handle effects were influenced by operator stature; taller participants received more benefit from Cot-2's stretcher-style handles; shoulder muscles' demands were greater for shorter participants due to handle location. Providing handle options and automatic leg folding/unfolding operation can reduce cot operator's effort and physical strain. Practitioner Summary: Paramedics frequently incur musculoskeletal injuries associated with patient-handling tasks. A controlled experiment was conducted to assess effects of ambulance cot design features on physical stress of operators, as seen through muscle activity and operator's perceptions. Differences between cots were found, signalling that intentional design can reduce operator's physical stress.

OpenCV and TYZX : video surveillance for tracking.

DOE Office of Scientific and Technical Information (OSTI.GOV)

He, Jim; Spencer, Andrew; Chu, Eric

2008-08-01

As part of the National Security Engineering Institute (NSEI) project, several sensors were developed in conjunction with an assessment algorithm. A camera system was developed in-house to track the locations of personnel within a secure room. In addition, a commercial, off-the-shelf (COTS) tracking system developed by TYZX was examined. TYZX is a Bay Area start-up that has developed its own tracking hardware and software which we use as COTS support for robust tracking. This report discusses the pros and cons of each camera system, how they work, a proposed data fusion method, and some visual results. Distributed, embedded image processingmore » solutions show the most promise in their ability to track multiple targets in complex environments and in real-time. Future work on the camera system may include three-dimensional volumetric tracking by using multiple simple cameras, Kalman or particle filtering, automated camera calibration and registration, and gesture or path recognition.« less

Training Issues Associated with COTS-based Information Intensive Systems.

ERIC Educational Resources Information Center

Farr, John V.; Verma, Dinesh

2002-01-01

A literature review and survey responses from 194 information technology and aerospace contractors identified methods and outcomes of training delivery. Results were used to develop a framework for evaluating and selecting commercial off-the-shelf systems (COTS) for operations and maintenance training. (Contains 11 references.) (SK)

Risk mitigation process for utilization of commercial off-the-shelf (COTS) parts in CCD camera for military applications

NASA Astrophysics Data System (ADS)

Ahmad, Anees; Batcheldor, Scott; Cannon, Steven C.; Roberts, Thomas E.

2002-09-01

This paper presents the lessons learned during the design and development of a high performance cooled CCD camera for military applications utilizing common commercial off the shelf (COTS) parts. Our experience showed that concurrent evaluation and testing of high risk COTS must be performed to assess their performance over the required temperature range and other special product requirements such as fuel vapor compatibility, EMI and shock susceptibility, etc. Technical, cost and schedule risks for COTS parts must also be carefully evaluated. The customer must be involved in the selection and evaluation of such parts so that the performance limitations of the selected parts are clearly understood. It is equally important to check with vendors on the availability and obsolescence of the COTS parts being considered since the electronic components are often replaced by newer, better and cheaper models in a couple of years. In summary, this paper addresses the major benefits and risks associated with using commercial and industrial parts in military products, and suggests a risk mitigation approach to ensure a smooth development phase, and predictable performance from the end product.

Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot.

PubMed

Wu, Binhui; Wilmouth, R C

2008-02-29

Cancer Osaka thyroid, also known as Tpl-2 (Cot) is a member of the MAP3K kinase family and plays a key role in the regulation of the immune response to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and tumour necrosis factor-alpha (TNF-alpha). A series of Cot constructs with an N-terminal 6xHis tag were transiently expressed in HEK293 cells: Cot(130-399) (kinase domain), Cot(1-388) (N-terminal and kinase domains), Cot(1-413), Cot(1-438) (containing a putative PEST sequence), Cot(1-457) (containing both PEST and degron sequences) and Cot(1-467) (full-length protein). These Cot proteins were pulled down using an anti-6xHis antibody and separated by 2D electrophoresis. The gels were silver-stained and 21 proteins were detected that did not appear, or had substantially reduced intensity, in the control sample. Three of these were identified by MS and MS/MS analysis as Hsp90, Hsp70 and Grp78. Hsp90 appeared to bind to the kinase domain of Cot and this interaction was further investigated using co-immuno-precipitation with both overexpressed Cot in HEK293 cells and endogenous Cot in Hela cells.

Evaluation of the Trajectory Operations Applications Software Task (TOAST). Volume 2: Interview transcripts

NASA Technical Reports Server (NTRS)

Perkins, Sharon; Martin, Andrea; Bavinger, Bill

1990-01-01

The Trajectory Operations Applications Software Task (TOAST) is a software development project whose purpose is to provide trajectory operation pre-mission and real-time support for the Space Shuttle. The purpose of the evaluation was to evaluate TOAST as an Application Manager - to assess current and planned capabilities, compare capabilities to commercially-available off the shelf (COTS) software, and analyze requirements of MCC and Flight Analysis Design System (FADS) for TOAST implementation. As a major part of the data gathering for the evaluation, interviews were conducted with NASA and contractor personnel. Real-time and flight design users, orbit navigation users, the TOAST developers, and management were interviewed. Code reviews and demonstrations were also held. Each of these interviews was videotaped and transcribed as appropriate. Transcripts were edited and are presented chronologically.

Solution identification and quantitative analysis of fiber-capacitive drop analyzer based on multivariate statistical methods

NASA Astrophysics Data System (ADS)

Chen, Zhe; Qiu, Zurong; Huo, Xinming; Fan, Yuming; Li, Xinghua

2017-03-01

A fiber-capacitive drop analyzer is an instrument which monitors a growing droplet to produce a capacitive opto-tensiotrace (COT). Each COT is an integration of fiber light intensity signals and capacitance signals and can reflect the unique physicochemical property of a liquid. In this study, we propose a solution analytical and concentration quantitative method based on multivariate statistical methods. Eight characteristic values are extracted from each COT. A series of COT characteristic values of training solutions at different concentrations compose a data library of this kind of solution. A two-stage linear discriminant analysis is applied to analyze different solution libraries and establish discriminant functions. Test solutions can be discriminated by these functions. After determining the variety of test solutions, Spearman correlation test and principal components analysis are used to filter and reduce dimensions of eight characteristic values, producing a new representative parameter. A cubic spline interpolation function is built between the parameters and concentrations, based on which we can calculate the concentration of the test solution. Methanol, ethanol, n-propanol, and saline solutions are taken as experimental subjects in this paper. For each solution, nine or ten different concentrations are chosen to be the standard library, and the other two concentrations compose the test group. By using the methods mentioned above, all eight test solutions are correctly identified and the average relative error of quantitative analysis is 1.11%. The method proposed is feasible which enlarges the applicable scope of recognizing liquids based on the COT and improves the concentration quantitative precision, as well.

Theoretical analysis of the transition-state spectrum of the cyclooctatetraene unimolecular reaction: Three degree-of-freedom model calculations

NASA Astrophysics Data System (ADS)

Yoshida, Takahiko; Tokizaki, Chihiro; Takayanagi, Toshiyuki

2015-08-01

A three degree-of-freedom potential energy surface of the cyclooctatetraene (COT) unimolecular reaction that can describe both ring-inversion (D2d ↔ D2d) and double bond-alternation (D4h ↔ D4h) processes was constructed using complete active space self-consistent field calculations. The potential energy surface was used to simulate the experimentally measured transition-state spectrum by calculating the photodetachment spectrum of the COT anion with time-dependent wave packet formalism. The calculated spectrum reproduces the experimental result well. We also analyzed wavefunction properties at spectral peak positions to understand the COT unimolecular reaction dynamics.

Clinical Operational Tolerance After Renal Transplantation

PubMed Central

Orlando, Giuseppe; Hematti, Peiman; Stratta, Robert J.; Burke, George W.; Di Cocco, Pierpaolo; Pisani, Francesco; Soker, Shay; Wood, Kathryn

2015-01-01

In solid organ transplantation, the achievement of an immunosuppression (IS)-free state [also referred to as clinical operational tolerance (COT)] represents the ultimate goal. Although COT is feasible and safe in selected cases after liver transplantation, it is an exceptional finding after other types of solid organ transplantation. In the field of renal transplantation (RT), approximately 100 cases of COT have been reported to date, mainly in patients who were not compliant with their immunosuppressive regimens or in individuals who had previously received a bone marrow transplant for hematological disorders. On the basis of promising results obtained in animal models, several tolerogenic protocols have been attempted in humans, but most have failed to achieve robust and stable COT after RT. Molecule-based regimens have been largely ineffective, whereas cell-based regimens have provided some encouraging results. In these latter regimens, apart from standard IS, patients usually receive perioperative infusion of donor bone marrow–derived stem cells, which are able to interact with the immune cells of the host and mitigate their response to engraftment. Unfortunately, most renal transplant patients who developed acute rejection—occurring either during the weaning protocol or after complete withdrawal of IS—eventually lost their grafts. Currently, the immune monitoring necessary for predicting the presence and persistence of donor-specific unresponsiveness is not available. Overall, the present review will provide a conceptual framework for COT and conclude that stable and robust COT after RT remains an elusive goal and that the different strategies attempted to date are not yet reproducibly safe or effective. PMID:21107102

Differentiating disseminated intravascular coagulation (DIC) with the fibrinolytic phenotype from coagulopathy of trauma and acute coagulopathy of trauma-shock (COT/ACOTS).

PubMed

Gando, S; Wada, H; Thachil, J

2013-05-01

Two concepts have been proposed for the hemostatic changes occurring early after trauma. Disseminated intravascular coagulation (DIC) with the fibrinolytic phenotype is characterized by activation of the coagulation pathways, insufficient anticoagulant mechanisms and increased fibrinolysis. Coagulopathy of trauma and acute coagulopathy of trauma-shock (COT/ACOTS) occurs as a result of increased activation of the thrombomodulin and protein C pathways, leading to the suppression of coagulation and activation of fibrinolysis. Despite the differences between these two conditions, independent consideration of COT/ACOTS from DIC with the fibrinolytic phenotype is probably incorrect. Robust diagnostic criteria based on its pathophysiology are required to establish COT/ACOTS as a new independent disease concept. In addition, the independency of its characteristics, laboratory data, time courses and prognosis from DIC should be confirmed. Confusion between two concepts may be based on studies of trauma lacking the following: (i) a clear distinction of the properties of blood between the inside and outside of vessels, (ii) a clear distinction between physiologic and pathologic hemostatic changes, (iii) attention to the time courses of the changes in hemostatic parameters, (iv) unification of the study population, and (v) recognition that massive bleeding is not synonymous with coagulation disorders. More information is needed to elucidate the pathogenesis of these two entities, DIC with the fibrinolytic phenotype and COT/ACOTS after trauma. However, available data suggest that COT/ACOTS is not a new concept but a disease entity similar to or the same as DIC with the fibrinolytic phenotype. © 2013 International Society on Thrombosis and Haemostasis.

System-on-chip-centric unattended embedded sensors in homeland security and defense applications

NASA Astrophysics Data System (ADS)

Jannson, Tomasz; Forrester, Thomas; Degrood, Kevin; Shih, Min-Yi; Walter, Kevin; Lee, Kang; Gans, Eric; Esterkin, Vladimir

2009-05-01

System-on-chip (SoC) single-die electronic integrated circuit (IC) integration has recently been attracting a great deal of attention, due to its high modularity, universality, and relatively low fabrication cost. The SoC also has low power consumption and it is naturally suited to being a base for integration of embedded sensors. Such sensors can run unattended, and can be either commercial off-the-shelf (COTS) electronic, COTS microelectromechanical systems (MEMS), or optical-COTS or produced in house (i.e., at Physical Optics Corporation, POC). In the version with the simplest electronic packaging, they can be integrated with low-power wireless RF that can communicate with a central processing unit (CPU) integrated in-house and installed on the specific platform of interest. Such a platform can be a human body (for e-clothing), unmanned aerial vehicle (UAV), unmanned ground vehicle (UGV), or many others. In this paper we discuss SoC-centric embedded unattended sensors in Homeland Security and military applications, including specific application scenarios (or CONOPS). In one specific example, we analyze an embedded polarization optical sensor produced in house, including generalized Lambertian light-emitting diode (LED) sources and secondary nonimaging optics (NIO).

Ontology for Life-Cycle Modeling of Water Distribution Systems: Model View Definition

DTIC Science & Technology

2013-06-01

Research and Development Center, Construction Engineering Research Laboratory (ERDC-CERL) to develop a life-cycle building model have resulted in the...Laboratory (ERDC-CERL) to develop a life-cycle building model have resulted in the definition of a “core” building information model that contains...developed experimental BIM models us- ing commercial off-the-shelf (COTS) software. Those models represent three types of typical low-rise Army

Increasing Open Source Software Integration on the Department of Defense Unclassified Desktop

DTIC Science & Technology

2008-06-01

free and legal access to the source code grants the user or operating agency considerable power and control . Commercial, off-the-shelf (COTS...COMMAND, CONTROL AND COMMUNICATIONS (C-3)) from the NAVAL POSTGRADUATE SCHOOL June 2008 Author: Steven A. Schearer Approved...Network. This fee also entitles users to unlimited web support with a two-business-day turnaround time. The retail price for a one year, basic

Information Sharing for Computing Trust Metrics on COTS Electronic Components

DTIC Science & Technology

2008-09-01

8 a. Standard SDLCs ...........................8 b. The Waterfall Model ......................9 c. V -shaped Model ...development of a system. There are many well-known SDLC models , the most popular of which are: • Waterfall • V -shaped • Spiral • Agile a. Standard...the SDLC or applied to software and hardware distribution chain. A. JØSANG’S MODEL DEFINED Jøsang expresses "opinions" mathematically as: 1

Operational Alignment in Predator Training Research

DTIC Science & Technology

2014-04-21

to the SO and, down the road, the Mission Intelligence Coordinator ( MIC ). 2. X-Plane 9 is COTS software and serves as the aeronautical simulation...uses to communicate with ground forces, command and control (C2) elements, and the Mission Intelligence Coordinator ( MIC ), among others. 10. LNCS...because key events can be scripted and initiated as appropriate, while distractor events like a villager walking around a market can be controlled by

Modular Rocket Engine Control Software (MRECS)

NASA Technical Reports Server (NTRS)

Tarrant, Charlie; Crook, Jerry

1997-01-01

The Modular Rocket Engine Control Software (MRECS) Program is a technology demonstration effort designed to advance the state-of-the-art in launch vehicle propulsion systems. Its emphasis is on developing and demonstrating a modular software architecture for a generic, advanced engine control system that will result in lower software maintenance (operations) costs. It effectively accommodates software requirements changes that occur due to hardware. technology upgrades and engine development testing. Ground rules directed by MSFC were to optimize modularity and implement the software in the Ada programming language. MRECS system software and the software development environment utilize Commercial-Off-the-Shelf (COTS) products. This paper presents the objectives and benefits of the program. The software architecture, design, and development environment are described. MRECS tasks are defined and timing relationships given. Major accomplishment are listed. MRECS offers benefits to a wide variety of advanced technology programs in the areas of modular software, architecture, reuse software, and reduced software reverification time related to software changes. Currently, the program is focused on supporting MSFC in accomplishing a Space Shuttle Main Engine (SSME) hot-fire test at Stennis Space Center and the Low Cost Boost Technology (LCBT) Program.

Adaptation of Control Center Software to Commerical Real-Time Display Applications

NASA Technical Reports Server (NTRS)

Collier, Mark D.

1994-01-01

NASA-Marshall Space Flight Center (MSFC) is currently developing an enhanced Huntsville Operation Support Center (HOSC) system designed to support multiple spacecraft missions. The Enhanced HOSC is based upon a distributed computing architecture using graphic workstation hardware and industry standard software including POSIX, X Windows, Motif, TCP/IP, and ANSI C. Southwest Research Institute (SwRI) is currently developing a prototype of the Display Services application for this system. Display Services provides the capability to generate and operate real-time data-driven graphic displays. This prototype is a highly functional application designed to allow system end users to easily generate complex data-driven displays. The prototype is easy to use, flexible, highly functional, and portable. Although this prototype is being developed for NASA-MSFC, the general-purpose real-time display capability can be reused in similar mission and process control environments. This includes any environment depending heavily upon real-time data acquisition and display. Reuse of the prototype will be a straight-forward transition because the prototype is portable, is designed to add new display types easily, has a user interface which is separated from the application code, and is very independent of the specifics of NASA-MSFC's system. Reuse of this prototype in other environments is a excellent alternative to creation of a new custom application, or for environments with a large number of users, to purchasing a COTS package.

Purification and kinetic characterization of recombinant human mitogen-activated protein kinase kinase kinase COT and the complexes with its cellular partner NF-kappa B1 p105.

PubMed

Jia, Yong; Quinn, Christopher M; Bump, Nancy J; Clark, Kevin M; Clabbers, Anca; Hardman, Jennifer; Gagnon, Andrew; Kamens, Joanne; Tomlinson, Medha J; Wishart, Neil; Allen, Hamish

2005-09-01

Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable form when produced in complex with the C-terminal half of p105. The k(cat) of COT 30--397 is reduced approximately 47--fold in the COT 30--467/p105 Delta N complex. COT prefers Mn(2+) to Mg(2+) as the ATP metal cofactor, exhibiting an unusually high ATP K(m) in the presence of Mg(2+). When using Mn(2+) as the cofactor, the ATP K(m) is reduced to a level typical of most kinases. In contrast, the binding affinity of COT for its other substrate MEK is cofactor independent. Our results using purified proteins indicate that p105 binding improves COT solubility and stability while down-regulating kinase activity, consistent with cellular data showing that p105 functions as an inhibitor of COT.

An Introduction to Flight Software Development: FSW Today, FSW 2010

NASA Technical Reports Server (NTRS)

Gouvela, John

2004-01-01

Experience and knowledge gained from ongoing maintenance of Space Shuttle Flight Software and new development projects including Cockpit Avionics Upgrade are applied to projected needs of the National Space Exploration Vision through Spiral 2. Lessons learned from these current activities are applied to create a sustainable, reliable model for development of critical software to support Project Constellation. This presentation introduces the technologies, methodologies, and infrastructure needed to produce and sustain high quality software. It will propose what is needed to support a Vision for Space Exploration that places demands on the innovation and productivity needed to support future space exploration. The technologies in use today within FSW development include tools that provide requirements tracking, integrated change management, modeling and simulation software. Specific challenges that have been met include the introduction and integration of Commercial Off the Shelf (COTS) Real Time Operating System for critical functions. Though technology prediction has proved to be imprecise, Project Constellation requirements will need continued integration of new technology with evolving methodologies and changing project infrastructure. Targets for continued technology investment are integrated health monitoring and management, self healing software, standard payload interfaces, autonomous operation, and improvements in training. Emulation of the target hardware will also allow significant streamlining of development and testing. The methodologies in use today for FSW development are object oriented UML design, iterative development using independent components, as well as rapid prototyping . In addition, Lean Six Sigma and CMMI play a critical role in the quality and efficiency of the workforce processes. Over the next six years, we expect these methodologies to merge with other improvements into a consolidated office culture with all processes being guided by automated office assistants. The infrastructure in use today includes strict software development and configuration management procedures, including strong control of resource management and critical skills coverage. This will evolve to a fully integrated staff organization with efficient and effective communication throughout all levels guided by a Mission-Systems Architecture framework with focus on risk management and attention toward inevitable product obsolescence. This infrastructure of computing equipment, software and processes will itself be subject to technological change and need for management of change and improvement,

Internetting tactical security sensor systems

NASA Astrophysics Data System (ADS)

Gage, Douglas W.; Bryan, W. D.; Nguyen, Hoa G.

1998-08-01

The Multipurpose Surveillance and Security Mission Platform (MSSMP) is a distributed network of remote sensing packages and control stations, designed to provide a rapidly deployable, extended-range surveillance capability for a wide variety of military security operations and other tactical missions. The baseline MSSMP sensor suite consists of a pan/tilt unit with video and FLIR cameras and laser rangefinder. With an additional radio transceiver, MSSMP can also function as a gateway between existing security/surveillance sensor systems such as TASS, TRSS, and IREMBASS, and IP-based networks, to support the timely distribution of both threat detection and threat assessment information. The MSSMP system makes maximum use of Commercial Off The Shelf (COTS) components for sensing, processing, and communications, and of both established and emerging standard communications networking protocols and system integration techniques. Its use of IP-based protocols allows it to freely interoperate with the Internet -- providing geographic transparency, facilitating development, and allowing fully distributed demonstration capability -- and prepares it for integration with the IP-based tactical radio networks that will evolve in the next decade. Unfortunately, the Internet's standard Transport layer protocol, TCP, is poorly matched to the requirements of security sensors and other quasi- autonomous systems in being oriented to conveying a continuous data stream, rather than discrete messages. Also, its canonical 'socket' interface both conceals short losses of communications connectivity and simply gives up and forces the Application layer software to deal with longer losses. For MSSMP, a software applique is being developed that will run on top of User Datagram Protocol (UDP) to provide a reliable message-based Transport service. In addition, a Session layer protocol is being developed to support the effective transfer of control of multiple platforms among multiple control stations.

High Performance, Dependable Multiprocessor

NASA Technical Reports Server (NTRS)

Ramos, Jeremy; Samson, John R.; Troxel, Ian; Subramaniyan, Rajagopal; Jacobs, Adam; Greco, James; Cieslewski, Grzegorz; Curreri, John; Fischer, Michael; Grobelny, Eric;

Using CORBA to integrate manufacturing cells to a virtual enterprise

NASA Astrophysics Data System (ADS)

Pancerella, Carmen M.; Whiteside, Robert A.

1997-01-01

It is critical in today's enterprises that manufacturing facilities are not isolated from design, planning, and other business activities and that information flows easily and bidirectionally between these activities. It is also important and cost-effective that COTS software, databases, and corporate legacy codes are well integrated in the information architecture. Further, much of the information generated during manufacturing must be dynamically accessible to engineering and business operations both in a restricted corporate intranet and on the internet. The software integration strategy in the Sandia Agile Manufacturing Testbed supports these enterprise requirements. We are developing a CORBA-based distributed object software system for manufacturing. Each physical machining device is a CORBA object and exports a common IDL interface to allow for rapid and dynamic insertion, deletion, and upgrading within the manufacturing cell. Cell management CORBA components access manufacturing devices without knowledge of any device-specific implementation. To support information flow from design to planning data is accessible to machinists on the shop floor. CORBA allows manufacturing components to be easily accessible to the enterprise. Dynamic clients can be created using web browsers and portable Java GUI's. A CORBA-OLE adapter allows integration to PC desktop applications. Other commercial software can access CORBA network objects in the information architecture through vendor API's.

Primary Care Clinic Re-Design for Prescription Opioid Management.

PubMed

Parchman, Michael L; Von Korff, Michael; Baldwin, Laura-Mae; Stephens, Mark; Ike, Brooke; Cromp, DeAnn; Hsu, Clarissa; Wagner, Ed H

The challenge of responding to prescription opioid overuse within the United States has fallen disproportionately on the primary care clinic setting. Here we describe a framework comprised of 6 Building Blocks to guide efforts within this setting to address the use of opioids for chronic pain. Investigators conducted site visits to thirty primary care clinics across the United States selected for their use of team-based workforce innovations. Site visits included interviews with leadership, clinic tours, observations of clinic processes and team meetings, and interviews with staff and clinicians. Data were reviewed to identify common attributes of clinic system changes around chronic opioid therapy (COT) management. These concepts were reviewed to develop narrative descriptions of key components of changes made to improve COT use. Twenty of the thirty sites had addressed improvements in COT prescribing. Across these sites a common set of 6 Building Blocks were identified: 1) providing leadership support; 2) revising and aligning clinic policies, patient agreements (contracts) and workflows; 3) implementing a registry tracking system; 4) conducting planned, patient-centered visits; 5) identifying resources for complex patients; and 6) measuring progress toward achieving clinic objectives. Common components of clinic policies, patient agreements and data tracked in registries to assess progress are described. In response to prescription opioid overuse and the resulting epidemic of overdose and addiction, primary care clinics are making improvements driven by a common set of best practices that address complex challenges of managing COT patients in primary care settings. © Copyright 2017 by the American Board of Family Medicine.

Assessing the effect of micro-lysimeters on NRWI: Do micro-lysimeters adequately represent the water input of natural soil?

NASA Astrophysics Data System (ADS)

Kidron, Giora J.; Kronenfeld, Rafael

2017-05-01

The use of micro-lysimeters (MLs) by the scientific community for the measurement of non-rainfall water input, NRWI (dew, fog, water vapor) has become more widespread. With MLs being isolated bodies, we hypothesized that changes in heat flux may affect the surface temperatures and subsequently NRWI. Measurements were conducted with MLs of various lengths (3.5, 12, 20, 30, 40, 50 cm for 2014 and 3.5, 12, 50 cm for 2015), and on the adjacent soil that served as a control (COT) using cloths attached to glass plates in Sede Boqer (Negev Desert, Israel) during the late summer and fall of 2014 and 2015. In addition, periodical temperature and moisture measurements were also conducted on additional lysimeters. Non-significant differences in NRWI characterized MLs 12-50 cm-long, which could have been therefore grouped (termed ML12/50). However, these lysimeters and especially the 3.5 cm-long ML (ML3.5) yielded substantially higher values than that of COT, with the ratio of ML12/50 to COT and the ratio of ML3.5 to COT being up to 2.4 and 5.8, respectively, implying, as was indeed found during periodic measurements, lower nocturnal temperatures and subsequently higher moisture content at 0-0.2 cm at the MLs in comparison to COT. This was also reflected in the amount of recorded mornings with effective (>0.03 mm) NRWI: 34 mornings based on the ML12/50 in comparison to only 4 when based on COT. The findings raise serious concerns regarding published data on NRWI and call for proper calibration between the amounts obtained by the MLs and the natural intact soil.

Component-Based Visualization System

NASA Technical Reports Server (NTRS)

Delgado, Francisco

2005-01-01

A software system has been developed that gives engineers and operations personnel with no "formal" programming expertise, but who are familiar with the Microsoft Windows operating system, the ability to create visualization displays to monitor the health and performance of aircraft/spacecraft. This software system is currently supporting the X38 V201 spacecraft component/system testing and is intended to give users the ability to create, test, deploy, and certify their subsystem displays in a fraction of the time that it would take to do so using previous software and programming methods. Within the visualization system there are three major components: the developer, the deployer, and the widget set. The developer is a blank canvas with widget menu items that give users the ability to easily create displays. The deployer is an application that allows for the deployment of the displays created using the developer application. The deployer has additional functionality that the developer does not have, such as printing of displays, screen captures to files, windowing of displays, and also serves as the interface into the documentation archive and help system. The third major component is the widget set. The widgets are the visual representation of the items that will make up the display (i.e., meters, dials, buttons, numerical indicators, string indicators, and the like). This software was developed using Visual C++ and uses COTS (commercial off-the-shelf) software where possible.

Commercial Off-The-Shelf (COTS) Program: Issues and Results of Upscreening COTS Parts for NASA Flight Hardware

NASA Technical Reports Server (NTRS)

Sandor, Mike; Agarwal, Shri; Villegas, Enrique

2000-01-01

This paper presents The Commercial Off-The-Shelf Program (COTS). The topics of discussion are: 1) Introduction of COTS; 2) MARS01 Program/Requirements; 3) MARS01 COTS Screening Flow; 4) Test Results-Electrical, C-Sam, Burn-In; 5) Value Added Analysis (Risk Reduction); 6) Value Added Analysis (Cost); 7) Impact of COTS ++ Screening; and 8) Summary. This paper is presented in viewgraph form.

NIK and Cot cooperate to trigger NF-kappaB p65 phosphorylation.

PubMed

Wittwer, Tobias; Schmitz, M Lienhard

2008-06-27

The serine/threonine kinase Cot triggers NF-kappaB-dependent transactivation and activation of various MAPKinases. Here we identify Cot as a novel p65 interacting protein kinase. Cot expression induces p65 phosphorylation at serines 536 and 468 in dependence from its kinase function. Accordingly, shRNA-mediated knockdown of Cot expression interferes with TNF-induced NF-kappaB-dependent gene expression. Also the C-terminally truncated, oncogenic form of Cot is able to trigger p65 phosphorylation. In vitro kinase assays and dominant negative mutants revealed that NIK functions downstream of Cot to mediate p65 phosphorylation.

TRAF6 and Src kinase activity regulates Cot activation by IL-1.

PubMed

Rodríguez, Cristina; Pozo, Maite; Nieto, Elvira; Fernández, Margarita; Alemany, Susana

2006-09-01

Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway under physiological conditions. Cot is activated by LPS, by inducing its dissociation from the inactive p105 NFkappaB-Cot complex in macrophages. Here, we show that IL-1 promotes a 10-fold increase in endogenous Cot activity and that Cot is the only MAP kinase kinase kinase that activates ERK1/ERK2 in response to this cytokine. Moreover, in cells where the expression of Cot is blocked, IL-1 fails to induce an increase in IL-8 and MIP-1betamRNA levels. The activation of Cot-MKK1-ERK1/ERK2 signalling pathway by IL-1 is dependent on the activity of the transducer protein TRAF6. Most important, IL-1-induced ERK1/ERK2 activation is inhibited by PP1, a known inhibitor of Src tyrosine kinases, but this tyrosine kinase activity is not required for IL-1 to activate other MAP kinases such as p38 and JNK. This Src kinases inhibitor does not block the dissociation and subsequently degradation of Cot in response to IL-1, indicating that other events besides Cot dissociation are required to activate Cot. All these data highlight the specific requirements for activation of the Cot-MKK1-ERK1/ERK2 pathway and provide evidence that Cot controls the functions of IL-1 that are mediated by ERK1/ERK2.

Assessing the potential for interaction between the insecticidal activity of two genetically engineered cotton events combined by conventional breeding: An example with COT102 × MON 15985.

PubMed

Levine, Steven L; Mueller, Geoffrey M; Uffman, Joshua P

2016-08-01

Bollgard(®) III was developed by combining cotton events COT102 and MON 15985 through conventional breeding to improve efficacy against lepidopteran feeding damage. COT102 produces the Vip3Aa19 protein and MON 15985 produces the Cry1Ac and Cry2Ab2 proteins. COT102 × MON 15985 has also been bred with Roundup Ready Flex(®) cotton (MON 88913) that confers glyphosate tolerance. This study evaluated the activity of COT102 and MON 15985 and the combined activity of COT102 and MON 15985 against the cotton bollworm (CBW, Helicoverpa zea). COT102, MON 15985, COT102 × MON 15985 and COT102 × MON 15985 × MON 88913 have comparable Vip3Aa19 and/or Cry1Ac, Cry2Ab2 protein expression levels as determined by enzyme-linked immunosorbent assay. CBW demonstrated concentration-dependent growth inhibition after 7-days of feeding on lyophilized leaf tissue derived from COT102, MON 15985, COT102 × MON 15985 and COT102 × MON 15985 × MON 88913 incorporated into an artificial diet. Observed EC50 values for COT102 × MON 15985 and COT102 × MON 15985 × MON 88913 were comparable (≤4% deviation) with the predicted EC50 value under the assumption of additivity using the combined activity of COT102 and MON 15985. No interaction in biological activity between COT102 and MON 15985 is consistent with results from competition and ligand blotting assays that demonstrated that Vip3Aa does not inhibit the binding of either Cry1Ac or Cry2Ab2 and vice versa. The results from this study demonstrate that the activity of COT102 × MON 15985 against CBW is consistent with predictions of additivity. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

Design and Evaluation of the MINTACS SeeTrack Exchange (MINSTE) Concept Demonstrator

DTIC Science & Technology

2009-04-01

software products . URL - http://www.esri.com/ 3 The Technical Cooperation Program (TTCP) is an international organisation that collaborates in defence...off-the-shelf (COTS) products . This provides a basis for implementing interoperability across application, vendor and organisation boundaries. XML...Network a suite of data analysis tools, such as ArcGIS products : 15 DSTO-GD-0574 “…represents a great opportunity for the bringing together of a COP

Interservice/Industry Training, Simulation and Education Conference Partnerships for Learning in the New Millennium Abstracts

DTIC Science & Technology

2000-01-01

for flight test data, and both generic and specialized tools of data filtering , data calibration, modeling , system identification, and simulation...GRAMMATICAL MODEL AND PARSER FOR AIR TRAFFIC CONTROLLER’S COMMANDS 11 A SPEECH-CONTROLLED INTERACTIVE VIRTUAL ENVIRONMENT FOR SHIP FAMILIARIZATION 12... MODELING AND SIMULATION IN THE 21ST CENTURY 23 NEW COTS HARDWARE AND SOFTWARE REDUCE THE COST AND EFFORT IN REPLACING AGING FLIGHT SIMULATORS SUBSYSTEMS

A Proven Ground System Architecture for Promoting Collaboration and Common Solutions at NASA

NASA Technical Reports Server (NTRS)

Smith, Danford

2005-01-01

Requirement: Improve how NASA develops and maintains ground data systems for dozens of missions, with a couple new missions always in the development phase. Decided in 2001 on enhanced message-bus architecture. Users offered choices for major components. They plug and play because key interfaces are all the same. Can support COTS, heritage, and new software. Even the middleware can be switched. Project name: GMSEC. Goddard Mission Services Evolution Center.

Real-Time Tomography Mooring

DTIC Science & Technology

1992-06-01

Vi th this sampling schedule the data logger has enough (data. storage capacity for, a five yea r deploymeneit-. SYsteim specifica tionis are shown...sit [I t ille check is pwrformed as a virttial device. called -Ilinei check". which is scheduled in the task tabhe aid(] executed by the systsenm cot...PCI as FSK carrier detect input- add function (5) to do timer/counter control - stop counter when defaults set I 6. Instrument scheduler software

In-Orbit Monitoring of Space Weather and Its Effects on Commercial- Off-The-Shelf (COTS) Electronics - A Decade of Research Using Micro-Satellites

NASA Astrophysics Data System (ADS)

Underwood, Craig I.; Sweeting, Martin, , Sir

2002-01-01

Over the past 20 years the University of Surrey has gained significant experience in the use of commercial-off-the-shelf (COTS) devices operating in low-Earth orbit through the design, manufacture, launch and operation of more than a dozen "UoSAT" micro-satellites. The deleterious effects of the ionising radiation environment is of particular concern when using COTS technologies in space, and over the last decade, particular emphasis has been given to a programme of monitoring "space weather" in terms of the high energy proton and heavy-ion cosmic-ray environment these spacecraft encounter, and to observing and analysing its effects - particularly with regard to single-event effects - upon the COTS devices on-board. The extended period of research has enabled a wide variety of conditions to be observed ranging across an entire solar cycle. This paper reports on the environment and effects observed, and describes the various methodologies that have been used to minimise the risk associated with the use of COTS devices in space. The practical importance of resilient error-detection and correction coding schemes to protect spacecraft data and control software is shown, as is the need for adequate levels of shielding against total ionising radiation dose. The relative effects of Galactic-Cosmic-Rays (GCRs), Solar Proton Events (SPEs) and trapped proton environments in Low-Earth orbit are discussed, and more recent flight data extends these observations out to very high orbit - approx 60,000 km altitude. As well as gaining practical data on space weather and its effects on advanced electronics, the research has resulted in the design and construction of a series of inexpensive, compact, and low- power particle detectors, which are capable of providing routine environmental "health" warnings for future operational spacecraft. Low cost micro-satellites have proven to be ideal vehicles for quick response and cost effective space technology verification missions, where environmental data has been gathered with regard to providing practical engineering data on systems' performance and reliability.

Phosphorylation of spore coat proteins by a family of atypical protein kinases

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nguyen, Kim B.; Sreelatha, Anju; Durrant, Eric S.

The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins. One member, family with sequence similarity 20C (Fam20C), is the physiological Golgi casein kinase. While examining distantly related protein sequences, we observed low levels of identity between the spore coat protein H (CotH), and the Fam20C-related secretory pathway kinases. CotH is a component of the spore in many bacterial and eukaryotic species, and is required for efficient germination of spores in Bacillus subtilis;more » however, the mechanism by which CotH affects germination is unclear. In this paper, we show that CotH is a protein kinase. The crystal structure of CotH reveals an atypical protein kinase-like fold with a unique mode of ATP binding. Examination of the genes neighboring cotH in B. subtilis led us to identify two spore coat proteins, CotB and CotG, as CotH substrates. Furthermore, we show that CotH-dependent phosphorylation of CotB and CotG is required for the efficient germination of B. subtilis spores. Finally and collectively, our results define a family of atypical protein kinases and reveal an unexpected role for protein phosphorylation in spore biology.« less

Phosphorylation of spore coat proteins by a family of atypical protein kinases

DOE PAGES

Nguyen, Kim B.; Sreelatha, Anju; Durrant, Eric S.; ...

2016-05-16

The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins. One member, family with sequence similarity 20C (Fam20C), is the physiological Golgi casein kinase. While examining distantly related protein sequences, we observed low levels of identity between the spore coat protein H (CotH), and the Fam20C-related secretory pathway kinases. CotH is a component of the spore in many bacterial and eukaryotic species, and is required for efficient germination of spores in Bacillus subtilis;more » however, the mechanism by which CotH affects germination is unclear. In this paper, we show that CotH is a protein kinase. The crystal structure of CotH reveals an atypical protein kinase-like fold with a unique mode of ATP binding. Examination of the genes neighboring cotH in B. subtilis led us to identify two spore coat proteins, CotB and CotG, as CotH substrates. Furthermore, we show that CotH-dependent phosphorylation of CotB and CotG is required for the efficient germination of B. subtilis spores. Finally and collectively, our results define a family of atypical protein kinases and reveal an unexpected role for protein phosphorylation in spore biology.« less

A Top-Down Performance Analysis of a Pegasus-Based Space Strike System

DTIC Science & Technology

1991-12-01

284): AP 1 O. coCO 4., cos 2 =~ +I Q ( QbO - 2) cos2 Cbo 28 This equation is useful for determining free-flight range for any set of burnout con...it may therefore be expressed in the manner given by Eq (26): cot1 - Qbo cos 2 Obo2 Qbo cos ¢ bo1 - cos2 kbo (27) Since \\/f1-cos7 Obo = sin Obo and...in general, cos a sin a = 1/2 x sin 2a, then Eq (27) can be further simplified to ’’ 2 cot - = 2csc20b, - cot Obo (28) 2 Qbo Rearranging tcrms results

Serine/threonine kinase, Cot/Tpl2, regulates renal cell apoptosis in ischaemia/reperfusion injury.

PubMed

Yaomura, Takaaki; Tsuboi, Naotake; Urahama, Yoshinori; Hobo, Akinori; Sugimoto, Kenji; Miyoshi, Jun; Matsuguchi, Tetsuya; Reiji, Kannagi; Matsuo, Seiichi; Yuzawa, Yukio

2008-10-01

Cot/Tpl2, a serine/threonine (Ser/Thr) protein kinase, has been classified as a member of the mitogen-activated protein kinase (MAPK) family, and is known to have a pleiotropic role. Many studies have reported the involvement of Cot/Tpl2, mainly as a member of the Toll-like receptor (TLR) 4 signalling pathway in lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha (TNF-alpha) production. At the same time, it is also related to the caspase-dependent apoptotic pathway. Thus, the role of Cot/Tpl2 in ischaemia/reperfusion injury (IRI) in which TNF-alpha and apoptosis are the major pathogenetic factors was studied. IRI was induced in wild type (Cot/Tpl2(+/+)) mice and in Cot/Tpl2-deficient (Cot/Tpl2(-/-)) mice. The extent of tubular injury and renal function were studied. TNF-alpha production, neutrophil infiltration and apoptosis were also compared between the two groups. Cot/Tpl2(-/-) mice had preserved renal function compared with wild type mice in IRI. Although Cot/Tpl2 was phosphorylated in IRI and in the cultured tubular epithelial cells (TEC) after stimulation with LPS and hydrogen peroxide, there were no significant differences in terms of TNF-alpha production, neutrophil infiltration or MAPK activation between Cot/Tpl2(+/+) and Cot/Tpl2(-/-) mice. In contrast, Cot/Tpl2(-/-) mice showed obviously reduced terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling positive cells and cleaved caspase-3 positive cells. Furthermore, Cot/Tpl2-deficient TECs demonstrated significantly less caspase-3 activation after hydrogen peroxide stimulation with comparable caspase-9 activation to wild type TEC. Cot/Tpl2 did not function as a member of MAPK family, but as a promoter of apoptosis in IRI. These results suggest that Cot/Tpl2 could be a possible therapeutic target in IRI.

COTS Ceramic Chip Capacitors: An Evaluation of the Parts and Assurance Methodologies

NASA Technical Reports Server (NTRS)

Brusse, Jay A.; Sampson, Michael J.

2004-01-01

Commercial-Off-The-Shelf (COTS) multilayer ceramic chip capacitors (MLCCs) are continually evolving to reduce physical size and increase volumetric efficiency. Designers of high reliability aerospace and military systems are attracted to these attributes of COTS MLCCs and would like to take advantage of them while maintaining the high standards for long-term reliable operation they are accustomed io when selecting military qualified established reliability (MIL-ER) MLCCs. However, MIL-ER MLCCs are not available in the full range of small chip sizes with high capacitance as found in today's COTS MLCCs. The objectives for this evaluation were to assess the long-term performance of small case size COTS MLCCs and to identify effective, lower-cost product assurance methodologies. Fifteen (15) lots of COTS X7R dielectric MLCCs from four (4) different manufacturers and two (2) MIL-ER BX dielectric MLCCs from two (2) of the same manufacturers were evaluated. Both 0805 and 0402 chip sizes were included. Several voltage ratings were tested ranging from a high of 50 volts to a low of 6.3 volts. The evaluation consisted of a comprehensive screening and qualification test program based upon MIL-PRF-55681 (i.e., voltage conditioning, thermal shock, moisture resistance, 2000-hour life test, etc.). In addition, several lot characterization tests were performed including Destructive Physical Analysis (DPA), Highly Accelerated Life Test (HALT) and Dielectric Voltage Breakdown Strength. The data analysis included a comparison of the 2000-hour life test results (used as a metric for long-term performance) relative to the screening and characterization test results. Results of this analysis indicate that the long-term life performance of COTS MLCCs is variable -- some lots perform well, some lots perform poorly. DPA and HALT were found to be promising lot characterization tests to identify substandard COTS MLCC lots prior to conducting more expensive screening and qualification tests. The results indicate that lot- specific screening and qualification are still recommended for high reliability applications. One significant and concerning observation is that MIL- type voltage conditioning (100 hours at twice rated voltage, 125 C) was not an effective screen in removing infant mortality parts for the particular lots of COTS MLCCs evaluated.

Deregulated activation of oncoprotein kinase Tpl2/Cot in HTLV-I-transformed T cells.

PubMed

Babu, Geetha; Waterfield, Michael; Chang, Mikyoung; Wu, Xuefeng; Sun, Shao-Cong

2006-05-19

Protein kinase Tpl2/Cot is encoded by a protooncogene that is cis-activated by retroviral insertion in murine T cell lymphomas. It has remained unclear whether this oncoprotein kinase is mutated or post-translationally activated in human cancer cells. We have shown here that Tpl2/Cot is constitutively activated in human leukemia cell lines transformed by the human T cell leukemia virus type I (HTLV-I). The kinase activity of Tpl2/Cot is normally suppressed through its physical interaction with an inhibitor, the NF-kappaB1 precursor protein p105. Interestingly, a large pool of Tpl2/Cot is liberated from p105 and exhibits constitutive kinase activity in HTLV-I-transformed T cells. In contrast to its labile property in normal cells, the pathologically activated Tpl2/Cot is remarkably stable. Further, whereas the physiological activation of Tpl2/Cot involves its long isoform, the HTLV-activated Tpl2/Cot is predominantly the short isoform. We have also shown that the HTLV-I-encoded Tax protein is able to activate Tpl2/Cot in transfected cells. Finally, Tpl2/Cot participates in the activation of NF-kappaB by Tax. These findings indicate that deregulated activation of Tpl2/Cot may occur in human cancer cells.

Lime Juice and Vinegar Injections as a Cheap and Natural Alternative to Control COTS Outbreaks.

PubMed

Moutardier, Grégoire; Gereva, Sompert; Mills, Suzanne C; Adjeroud, Mehdi; Beldade, Ricardo; Ham, Jayven; Kaku, Rocky; Dumas, Pascal

2015-01-01

Outbreaks of the corallivorous crown-of-thorns seastar Acanthaster planci (COTS) represent one of the greatest disturbances to coral reef ecosystems in the Indo-Pacific, affecting not only coral reefs but also the coastal communities which rely on their resources. While injection approaches are increasingly used in an attempt to control COTS densities, most of them display severe drawbacks including logistical challenges, high residual environmental impacts or low cost-effectiveness. We tested a new alternative control method based upon acidic injections of cheap, 100% natural products. We investigated the lethal doses, intra- and inter-specific disease transmission and immune responses of COTS when injected with fresh lime juice (extracted from local Citrus arantifolia) and white spirit vinegar. High COTS mortality was achieved with small volumes: 10-20 ml per seastar induced death in 89%/97% of injected specimens after an average 34.3 h/29.8 h for lime juice and vinegar respectively. Highest efficiency was reached for both solutions with double shots of (2 × 10 ml) in two different areas on the body: 100% mortality occurred within 12-24 h, which is similar or faster compared with other current injection methods. Multiple immune measures suggested that death was very likely caused by pH stress from the acidic solutions rather than a bacterial infection. Contagion to either conspecifics or a variety of other reef species was not observed, even at COTS densities 15 times higher than the highest naturally reported. 10 to 20 l lime juice/vinegar could kill up to a thousand COTS at a cost of less than 0.05 USD per specimen; no permits or special handling procedures are required. We conclude that injections of lime juice and vinegar offer great advantages when compared to current best practises and constitute a cheap and natural option for all reefs affected by COTS.

Lime Juice and Vinegar Injections as a Cheap and Natural Alternative to Control COTS Outbreaks

PubMed Central

Moutardier, Grégoire; Gereva, Sompert; Mills, Suzanne C.; Adjeroud, Mehdi; Beldade, Ricardo; Ham, Jayven; Kaku, Rocky; Dumas, Pascal

2015-01-01

Outbreaks of the corallivorous crown-of-thorns seastar Acanthaster planci (COTS) represent one of the greatest disturbances to coral reef ecosystems in the Indo-Pacific, affecting not only coral reefs but also the coastal communities which rely on their resources. While injection approaches are increasingly used in an attempt to control COTS densities, most of them display severe drawbacks including logistical challenges, high residual environmental impacts or low cost-effectiveness. We tested a new alternative control method based upon acidic injections of cheap, 100% natural products. We investigated the lethal doses, intra- and inter-specific disease transmission and immune responses of COTS when injected with fresh lime juice (extracted from local Citrus arantifolia) and white spirit vinegar. High COTS mortality was achieved with small volumes: 10–20 ml per seastar induced death in 89%/97% of injected specimens after an average 34.3 h/29.8 h for lime juice and vinegar respectively. Highest efficiency was reached for both solutions with double shots of (2 × 10 ml) in two different areas on the body: 100% mortality occurred within 12–24 h, which is similar or faster compared with other current injection methods. Multiple immune measures suggested that death was very likely caused by pH stress from the acidic solutions rather than a bacterial infection. Contagion to either conspecifics or a variety of other reef species was not observed, even at COTS densities 15 times higher than the highest naturally reported. 10 to 20 l lime juice/vinegar could kill up to a thousand COTS at a cost of less than 0.05 USD per specimen; no permits or special handling procedures are required. We conclude that injections of lime juice and vinegar offer great advantages when compared to current best practises and constitute a cheap and natural option for all reefs affected by COTS. PMID:26356840

Radiation-Hardened Solid-State Drive

NASA Technical Reports Server (NTRS)

Sheldon, Douglas J.

2010-01-01

A method is provided for a radiationhardened (rad-hard) solid-state drive for space mission memory applications by combining rad-hard and commercial off-the-shelf (COTS) non-volatile memories (NVMs) into a hybrid architecture. The architecture is controlled by a rad-hard ASIC (application specific integrated circuit) or a FPGA (field programmable gate array). Specific error handling and data management protocols are developed for use in a rad-hard environment. The rad-hard memories are smaller in overall memory density, but are used to control and manage radiation-induced errors in the main, and much larger density, non-rad-hard COTS memory devices. Small amounts of rad-hard memory are used as error buffers and temporary caches for radiation-induced errors in the large COTS memories. The rad-hard ASIC/FPGA implements a variety of error-handling protocols to manage these radiation-induced errors. The large COTS memory is triplicated for protection, and CRC-based counters are calculated for sub-areas in each COTS NVM array. These counters are stored in the rad-hard non-volatile memory. Through monitoring, rewriting, regeneration, triplication, and long-term storage, radiation-induced errors in the large NV memory are managed. The rad-hard ASIC/FPGA also interfaces with the external computer buses.

Modular Rocket Engine Control Software (MRECS)

NASA Technical Reports Server (NTRS)

Tarrant, C.; Crook, J.

1998-01-01

The Modular Rocket Engine Control Software (MRECS) Program is a technology demonstration effort designed to advance the state-of-the-art in launch vehicle propulsion systems. Its emphasis is on developing and demonstrating a modular software architecture for advanced engine control systems that will result in lower software maintenance (operations) costs. It effectively accommodates software requirement changes that occur due to hardware technology upgrades and engine development testing. Ground rules directed by MSFC were to optimize modularity and implement the software in the Ada programming language. MRECS system software and the software development environment utilize Commercial-Off-the-Shelf (COTS) products. This paper presents the objectives, benefits, and status of the program. The software architecture, design, and development environment are described. MRECS tasks are defined and timing relationships given. Major accomplishments are listed. MRECS offers benefits to a wide variety of advanced technology programs in the areas of modular software architecture, reuse software, and reduced software reverification time related to software changes. MRECS was recently modified to support a Space Shuttle Main Engine (SSME) hot-fire test. Cold Flow and Flight Readiness Testing were completed before the test was cancelled. Currently, the program is focused on supporting NASA MSFC in accomplishing development testing of the Fastrac Engine, part of NASA's Low Cost Technologies (LCT) Program. MRECS will be used for all engine development testing.

Optical properties of aerosol contaminated cloud derived from MODIS instrument

NASA Astrophysics Data System (ADS)

Mei, Linlu; Rozanov, Vladimir; Lelli, Luca; Vountas, Marco; Burrows, John P.

2016-04-01

The presence of absorbing aerosols above/within cloud can reduce the amount of up-welling radiation in visible (VIS) and short-wave infrared and darken the spectral reflectance when compared with a spectrum of a clean cloud observed by satellite instruments (Jethva et al., 2013). Cloud properties retrieval for aerosol contaminated cases is a great challenge. Even small additional injection of aerosol particles into clouds in the cleanest regions of Earth's atmosphere will cause significant effect on those clouds and on climate forcing (Koren et al., 2014; Rosenfeld et al., 2014) because the micro-physical cloud process are non-linear with respect to the aerosol loading. The current cloud products like Moderate Resolution Imaging Spectroradiometer (MODIS) ignoring the aerosol effect for the retrieval, which may cause significant error in the satellite-derived cloud properties. In this paper, a new cloud properties retrieval method, considering aerosol effect, based on the weighting-function (WF) method, is presented. The retrieval results shows that the WF retrieved cloud properties (e.g COT) agrees quite well with MODIS COT product for relative clear atmosphere (AOT ≤ 0.4) while there is a large difference for large aerosol loading. The MODIS COT product is underestimated for at least 2 - 3 times for AOT>0.4, and this underestimation increases with the increase of AOT.

Sohbrit: Autonomous COTS System for Satellite Characterization

NASA Astrophysics Data System (ADS)

Blazier, N.; Tarin, S.; Wells, M.; Brown, N.; Nandy, P.; Woodbury, D.

As technology continues to improve, driving down the cost of commercial astronomical products while increasing their capabilities, manpower to run observations has become the limiting factor in acquiring continuous and repeatable space situational awareness data. Sandia National Laboratories set out to automate a testbed comprised entirely of commercial off-the-shelf (COTS) hardware for space object characterization (SOC) focusing on satellites in geosynchronous orbit. Using an entirely autonomous system allows collection parameters such as target illumination and nightly overlap to be accounted for habitually; this enables repeatable development of target light curves to establish patterns of life in a variety of spectral bands. The system, known as Sohbrit, is responsible for autonomously creating an optimized schedule, checking the weather, opening the observatory dome, aligning and focusing the telescope, executing the schedule by slewing to each target and imaging it in a number of spectral bands (e.g., B, V, R, I, wide-open) via a filter wheel, closing the dome at the end of observations, processing the data, and storing/disseminating the data for exploitation via the web. Sohbrit must handle various situations such as weather outages and focus changes due to temperature shifts and optical seeing variations without human interaction. Sohbrit can collect large volumes of data nightly due to its high level of automation. To store and disseminate these large quantities of data, we utilize a cloud-based big data architecture called Firebird, which exposes the data out to the community for use by developers and analysts. Sohbrit is the first COTS system we are aware of to automate the full process of multispectral geosynchronous characterization from scheduling all the way to processed, disseminated data. In this paper we will discuss design decisions, issues encountered and overcome during implementation, and show results produced by Sohbrit.

Uncertainties in Cloud Phase and Optical Thickness Retrievals from the Earth Polychromatic Imaging Camera (EPIC)

NASA Technical Reports Server (NTRS)

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2016-01-01

This paper presents an investigation of the expected uncertainties of a single channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud-temperature-threshold-based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODIS daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single channel COT retrieval is feasible for EPIC. For ice clouds, single channel retrieval errors are minimal (less than 2 percent) due to the particle- size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10 percent, although for thin clouds (COT less than 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study.

Uncertainties in cloud phase and optical thickness retrievals from the Earth Polychromatic Imaging Camera (EPIC)

PubMed Central

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2018-01-01

This paper presents an investigation of the expected uncertainties of a single channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud temperature threshold based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODIS daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single channel COT retrieval is feasible for EPIC. For ice clouds, single channel retrieval errors are minimal (< 2%) due to the particle size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10%, although for thin clouds (COT < 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study. PMID:29619116

Uncertainties in cloud phase and optical thickness retrievals from the Earth Polychromatic Imaging Camera (EPIC).

PubMed

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2016-01-01

This paper presents an investigation of the expected uncertainties of a single channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud temperature threshold based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODIS daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single channel COT retrieval is feasible for EPIC. For ice clouds, single channel retrieval errors are minimal (< 2%) due to the particle size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10%, although for thin clouds (COT < 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study.

Uncertainties in cloud phase and optical thickness retrievals from the Earth Polychromatic Imaging Camera (EPIC)

NASA Astrophysics Data System (ADS)

Meyer, Kerry; Yang, Yuekui; Platnick, Steven

2016-04-01

This paper presents an investigation of the expected uncertainties of a single-channel cloud optical thickness (COT) retrieval technique, as well as a simple cloud-temperature-threshold-based thermodynamic phase approach, in support of the Deep Space Climate Observatory (DSCOVR) mission. DSCOVR cloud products will be derived from Earth Polychromatic Imaging Camera (EPIC) observations in the ultraviolet and visible spectra. Since EPIC is not equipped with a spectral channel in the shortwave or mid-wave infrared that is sensitive to cloud effective radius (CER), COT will be inferred from a single visible channel with the assumption of appropriate CER values for liquid and ice phase clouds. One month of Aqua MODerate-resolution Imaging Spectroradiometer (MODIS) daytime granules from April 2005 is selected for investigating cloud phase sensitivity, and a subset of these granules that has similar EPIC Sun-view geometry is selected for investigating COT uncertainties. EPIC COT retrievals are simulated with the same algorithm as the operational MODIS cloud products (MOD06), except using fixed phase-dependent CER values. Uncertainty estimates are derived by comparing the single-channel COT retrievals with the baseline bi-spectral MODIS retrievals. Results show that a single-channel COT retrieval is feasible for EPIC. For ice clouds, single-channel retrieval errors are minimal (< 2 %) due to the particle size insensitivity of the assumed ice crystal (i.e., severely roughened aggregate of hexagonal columns) scattering properties at visible wavelengths, while for liquid clouds the error is mostly limited to within 10 %, although for thin clouds (COT < 2) the error can be higher. Potential uncertainties in EPIC cloud masking and cloud temperature retrievals are not considered in this study.

Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat.

PubMed

Martins, Ligia O; Soares, Claudio M; Pereira, Manuela M; Teixeira, Miguel; Costa, Teresa; Jones, George H; Henriques, Adriano O

2002-05-24

The Bacillus subtilis endospore coat protein CotA shows laccase activity. By using comparative modeling techniques, we were able to derive a model for CotA based on the known x-ray structures of zucchini ascorbate oxidase and Cuprinus cereneus laccase. This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3). Single amino acid substitutions in the CotA T1 copper center (H497A, or M502L) did not prevent assembly of the mutant proteins into the coat and did not alter the pattern of extractable coat polypeptides. However, in contrast to a wild type strain, both mutants produced unpigmented colonies and spores unable to oxidize syringaldazine (SGZ) and 2'2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The CotA protein was purified to homogeneity from an overproducing Escherichia coli strain. The purified CotA shows an absorbance and a EPR spectra typical of blue multicopper oxidases. Optimal enzymatic activity was found at < or =pH 3.0 and at pH 7.0 for ABTS or SGZ oxidation, respectively. The apparent K(m) values for ABTS and SGZ at 37 degrees C were of 106 +/- 11 and 26 +/- 2 microm, respectively, with corresponding k(cat) values of 16.8 +/- 0.8 and 3.7 +/- 0.1 s(-1). Maximal enzyme activity was observed at 75 degrees C with ABTS as substrate. Remarkably, the coat-associated or the purified enzyme showed a half-life of inactivation at 80 degrees C of about 4 and 2 h, respectively, indicating that CotA is intrinsically highly thermostable.

Big Science, Small-Budget Space Experiment Package Aka MISSE-5: A Hardware And Software Perspective

NASA Technical Reports Server (NTRS)

Krasowski, Michael; Greer, Lawrence; Flatico, Joseph; Jenkins, Phillip; Spina, Dan

2007-01-01

Conducting space experiments with small budgets is a fact of life for many design groups with low-visibility science programs. One major consequence is that specialized space grade electronic components are often too costly to incorporate into the design. Radiation mitigation now becomes more complex as a result of being restricted to the use of commercial off-the-shelf (COTS) parts. Unique hardware and software design techniques are required to succeed in producing a viable instrument suited for use in space. This paper highlights some of the design challenges and associated solutions encountered in the production of a highly capable, low cost space experiment package.

A Hardware and Software Perspective of the Fifth Materials on the International Space Station Experiment (MISSE-5)

NASA Technical Reports Server (NTRS)

Krasowski, Michael; Greer, Lawrence; Flatico, Joseph; Jenkins, Phillip; Spina, Dan

2005-01-01

Conducting space experiments with small budgets is a fact of life for many design groups with low-visibility science programs. One major consequence is that specialized space grade electronic components are often too costly to incorporate into the design. Radiation mitigation now becomes more complex as a result of being restricted to the use of commercial off-the-shelf (COTS) parts. Unique hardware and software design techniques are required to succeed in producing a viable instrument suited for use in space. This paper highlights some of the design challenges and associated solutions encountered in the production of a highly capable, low cost space experiment package.

Enhanced International Space Station Ku-Band Telemetry Service

NASA Technical Reports Server (NTRS)

Cecil, Andrew J.; Pitts, R. Lee; Welch, Steven J.; Bryan, Jason D.

2014-01-01

The International Space Station (ISS) is in an operational configuration. To fully utilize the ISS and take advantage of the modern protocols and updated Ku-band access, the Huntsville Operations Support Center (HOSC) has designed an approach to extend the Kuband forward link access for payload investigators to their on-orbit payloads. This dramatically increases the ground to ISS communications for those users. This access also enables the ISS flight controllers operating in the Payload Operations and Integration Center to have more direct control over the systems they are responsible for managing and operating. To extend the Ku-band forward link to the payload user community the development of a new command server is necessary. The HOSC subsystems were updated to process the Internet Protocol Encapsulated packets, enable users to use the service based on their approved services, and perform network address translation to insure that the packets are forwarded from the user to the correct payload repeating that process in reverse from ISS to the payload user. This paper presents the architecture, implementation, and lessons learned. This will include the integration of COTS hardware and software as well as how the device is incorporated into the operational mission of the ISS. Thus, this paper also discusses how this technology can be applicable to payload users of the ISS.

Practice variation in the transfer of premature infants from incubators to open cots in Australian and New Zealand neonatal nurseries: results of an electronic survey.

PubMed

New, Karen; Bogossian, Fiona; East, Christine; Davies, Mark William

2010-06-01

The incubator environment is essential for optimal physiological functioning and development of the premature infant but the infant is ultimately required to make a successful transfer from incubator to open cot in order to be discharged from hospital. Criteria for transfer lack a systematic approach because no clear, specific guideline predominates in clinical practice. Practice variation exists between continents, regions and nurseries in the same countries, but there is no recent review of current practices utilised for transferring premature infants from incubators to open cots. To document current practice for transferring premature infants to open cots in neonatal nurseries. A descriptive, cross-sectional survey. Twenty-two neonatal intensive care units and fifty-six high dependency special care baby units located in public hospitals in Australia and New Zealand. A sample of 78 key clinical nursing leaders (nurse unit managers, clinical nurse consultants or clinical nurse specialists) within neonatal nurseries identified through email or telephone contact. Data were collected using a web-based survey on practice, decision-making and strategies utilised for transferring premature infants from incubators to open cots. Descriptive statistics (frequencies and crosstabs) were used to analyse data. Comparisons between groups were tested for statistical significance using Chi-squared or Fisher's exact test. Significant practice variation between countries was found for only one variable, nursing infants clothed (p=0.011). Processes and practices undertaken similarly in both countries include use of incubator air control mode, current weight criterion, thermal challenging, single-walled incubators and heated mattress systems. Practice variation was significant between neonatal intensive care units and special care baby units for weight range (p=0.005), evidence-based practice (p=0.004), historical nursery practice (p=0.029) and incubator air control mode (p=0.001). Differences in these variables were also found between nurseries in metropolitan and rural locations. Practice variation exists however; many practices are uniformly performed throughout neonatal nurseries in Australian and New Zealand. Commonality was seen between countries and in nurseries with a neonatal intensive care unit. Variation was significant between neonatal intensive care units and special care baby units and nurseries in metropolitan and rural locations. (c) 2009 Elsevier Ltd. All rights reserved.

From Mario to FIFA: What Qualitative Case Study Research Suggests about Games-Based Learning in a US Classroom

ERIC Educational Resources Information Center

Gerber, Hannah R.; Abrams, Sandra Schamroth; Onwuegbuzie, Anthony J.; Benge, Cindy L.

2014-01-01

This article explores the impact of using commercial-off-the-shelf (COTS) videogames in a high school curriculum when developed through a connected learning frame by examining the influence that COTS videogames have on transforming students' literacy learning in-school. However, it must be noted that transforming literacy in school is about more…

Finding the right time for weaning off immunosuppression in solid organ transplant recipients.

PubMed

Orlando, Giuseppe

2010-11-01

Solid organ transplantation (SOT) requires lifelong immunosuppression (IS) to prevent rejection and graft loss. The currently adopted immunosuppressive protocols are numerous and are based on the administration of at least two molecules with diverse mechanisms of action. Owing to the fact that the majority of immunosuppressants act non-selectively, the immune system is normally oversuppressed, and as a result is less able to both defend the host against infection and to control the spread of malignant cells. Consequently, long-term IS is burdened by chronic toxicity, which may be highly invalidating and may significantly influence patient's quality of life, compliance to treatment, overall success rate, and patient and graft survival. In an ideal scenario, SOT recipients should initially receive just enough IS to favor the onset of clinical operational tolerance (COT), a condition where the immune system of the host does not attack the graft in the absence of any immunosuppressant. COT has been documented after liver transplantation (LT) and renal transplantation (RT). First, COT was accidentally detected in patients who were nonadherent to treatment and who spontaneously decided to stop all IS without any medical guidance or surveillance. Later, it was described in recipients who required IS withdrawal following the occurrence of malignant diseases. Based on strikingly convincing experimental data, several tolerogenic protocols have recently been applied in patients but overall the results have been disappointing. The current literature demonstrates that COT can be safely achieved in stable LT recipients, with completely different strategies. Importantly, the onset of an episode of acute rejection during the attempt of IS withdrawal would not worsen the clinical outcome. On the contrary, COT remains a major challenge after RT because the onset of acute rejection will substantiate in graft loss. Currently, a major field of investigation aims to define markers of COT, which will allow the selection of individuals who are more prone to develop COT. Preliminary results in both RT and LT have just been announced; however, these markers will require validation in prospective studies.

CotA of Bacillus subtilis Is a Copper-Dependent Laccase

PubMed Central

Hullo, Marie-Françoise; Moszer, Ivan; Danchin, Antoine; Martin-Verstraete, Isabelle

2001-01-01

The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA nor other sporulation proteins are involved. We demonstrate that CotA is a laccase. Syringaldazine, a specific substrate of laccases, reacted with wild-type spores but not with ΔcotA spores. CotA may participate in the biosynthesis of the brown spore pigment, which appears to be a melanin-like product and to protect against UV light. PMID:11514528

Degraded Operational Environment: Integration of Social Network Infrastructure Concept in a Traditional Military C2 System

DTIC Science & Technology

2013-06-01

Communication Applet) UNIGE – D.I.M.E. Using a free application as “MIT APP Inventor” Android Software Development Kit DEGRADED C2 ICCRTS 2013...operate on an Android operating system up-gradable on which will be developed a simplified ACA ( Android Communication Applet) that will call C24U...Server) IP number . . . Portable COTS Devices ACA - C24U ( Android Communication Applet) Sending/receiving SEFL (Simple Exchange

Pharmacokinetics of cotinine in rats: a potential therapeutic agent for disorders of cognitive function

PubMed Central

Li, Pei; Beck, Wayne D.; Callahan, Patrick M.; Terry, Alvin V.; Bartlett, Michael G.

2016-01-01

Background Attention has been paid to cotinine (COT), one of the major metabolites of nicotine (NIC), for its pro-cognitive effects and potential therapeutic activities against Alzheimer's Disease (AD) and other types of cognitive impairment. In order to facilitate pharmacological and toxicological studies on COT for its pro-cognitive activities, we conducted a pharmacokinetic (PK) study of COT in rats, providing important oral and intravenously (IV) PK information. Methods In this study, plasma samples were obtained up to 48 hours after COT was dosed to rats orally and IV at a dose of 3 mg/kg. Plasma samples were prepared and analyzed using a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) bioanalytical method, providing concentration profiles of COT and metabolites after oral and IV administrations. Results The data were fitted into a one-compartment model and a two-compartment model for the oral and IV groups, respectively, providing important PK information for COT including PK profiles, half-life, clearance and bioavailability. The results suggested fast absorption, slow elimination and high bioavailability of COT in rats. Conclusions Several important facts about the PK properties in rats suggested COT could be a potential pro-cognitive agent. Information about the pharmacokinetics of COT in rats revealed in this study is of great importance for the future studies on COT or potential COT analogues as agents for improving cognition. PMID:25933960

Photoelectron spectroscopic study of the anionic transition metalorganic complexes [Fe(1,2)(COT)](-) and [Co(COT)](-).

PubMed

Li, Xiang; Eustis, Soren N; Bowen, Kit H; Kandalam, Anil

2008-09-28

The gas-phase, iron and cobalt cyclooctatetraene cluster anions, [Fe(1,2)(COT)](-) and [Co(COT)](-), were generated using a laser vaporization source and studied using mass spectrometry and anion photoelectron spectroscopy. Density functional theory was employed to compute the structures and spin multiplicities of these cluster anions as well as those of their corresponding neutrals. Both experimental and theoretically predicted electron affinities and photodetachment transition energies are in good agreement, authenticating the structures and spin multiplicities predicted by theory. The implied spin magnetic moments of these systems suggest that [Fe(COT)], [Fe(2)(COT)], and [Co(COT)] retain the magnetic moments of the Fe atom, the Fe(2) dimer, and the Co atom, respectively. Thus, the interaction of these transition metal, atomic and dimeric moieties with a COT molecule does not quench their magnetic moments, leading to the possibility that these combinations may be useful in forming novel magnetic materials.

Using Health Information Technology to Improve Adherence to Opioid Prescribing Guidelines in Primary Care

PubMed Central

Zlateva, Ianita; Khatri, Khushbu; Ciaburri, Nicholas

2015-01-01

Objective: To evaluate the impact of a clinical dashboard for opioid analgesic management on opioid prescribing and adherence to opioid practice guidelines in primary care. Methods: A pre/postimplementation evaluation using electronic health record (EHR) data from patients receiving chronic opioid therapy (COT) between April 1, 2011 and March 31, 2013. Measures include annual proportions of COT patients who received urine drug testing, signed an opioid treatment agreement, had a documented assessment of pain-related functional status, and had at least 1 visit with a behavioral health provider. Results: Adherence to several opioid prescribing guidelines improved in the postimplementation year compared with the preimplementation year: (1) the proportions of COT patients with a signed opioid treatment agreement and urine drug testing increased from 49% to 63% and 66% to 86%, respectively. The proportion of COT patients with a documented assessment of functional status increased from 33% to 46% and those with a behavioral health visit increased from 24% to 28%. However, there was a small decline in the proportion of patients prescribed COT from 3.4% to 3.1%. Discussion: Implementation of an opioid dashboard led to increased adherence to certain opioid practice guidelines and a decline in COT. This may be attributable to more efficient team-based pain management facilitated by the dashboard and increased transparency of opioid prescription practices. Health Information Technology solutions such as clinical dashboards can increase adherence to practice guidelines. PMID:25411860

Applying Reflective Middleware Techniques to Optimize a QoS-enabled CORBA Component Model Implementation

NASA Technical Reports Server (NTRS)

Wang, Nanbor; Parameswaran, Kirthika; Kircher, Michael; Schmidt, Douglas

2003-01-01

Although existing CORBA specifications, such as Real-time CORBA and CORBA Messaging, address many end-to-end quality-of service (QoS) properties, they do not define strategies for configuring these properties into applications flexibly, transparently, and adaptively. Therefore, application developers must make these configuration decisions manually and explicitly, which is tedious, error-prone, and open sub-optimal. Although the recently adopted CORBA Component Model (CCM) does define a standard configuration framework for packaging and deploying software components, conventional CCM implementations focus on functionality rather than adaptive quality-of-service, which makes them unsuitable for next-generation applications with demanding QoS requirements. This paper presents three contributions to the study of middleware for QoS-enabled component-based applications. It outlines rejective middleware techniques designed to adaptively (1) select optimal communication mechanisms, (2) manage QoS properties of CORBA components in their contain- ers, and (3) (re)con$gure selected component executors dynamically. Based on our ongoing research on CORBA and the CCM, we believe the application of rejective techniques to component middleware will provide a dynamically adaptive and (re)configurable framework for COTS software that is well-suited for the QoS demands of next-generation applications.

Applying Reflective Middleware Techniques to Optimize a QoS-enabled CORBA Component Model Implementation

NASA Technical Reports Server (NTRS)

Wang, Nanbor; Kircher, Michael; Schmidt, Douglas C.

2000-01-01

Although existing CORBA specifications, such as Real-time CORBA and CORBA Messaging, address many end-to-end quality-of-service (QoS) properties, they do not define strategies for configuring these properties into applications flexibly, transparently, and adaptively. Therefore, application developers must make these configuration decisions manually and explicitly, which is tedious, error-prone, and often sub-optimal. Although the recently adopted CORBA Component Model (CCM) does define a standard configuration frame-work for packaging and deploying software components, conventional CCM implementations focus on functionality rather than adaptive quality-of service, which makes them unsuitable for next-generation applications with demanding QoS requirements. This paper presents three contributions to the study of middleware for QoS-enabled component-based applications. It outlines reflective middleware techniques designed to adaptively: (1) select optimal communication mechanisms, (2) man- age QoS properties of CORBA components in their containers, and (3) (re)configure selected component executors dynamically. Based on our ongoing research on CORBA and the CCM, we believe the application of reflective techniques to component middleware will provide a dynamically adaptive and (re)configurable framework for COTS software that is well-suited for the QoS demands of next-generation applications.

Functional Loss of Bmsei Causes Thermosensitive Epilepsy in Contractile Mutant Silkworm, Bombyx mori

NASA Astrophysics Data System (ADS)

Nie, Hongyi; Cheng, Tingcai; Huang, Xiaofeng; Zhou, Mengting; Zhang, Yinxia; Dai, Fangyin; Mita, Kazuei; Xia, Qingyou; Liu, Chun

2015-07-01

The thermoprotective mechanisms of insects remain largely unknown. We reported the Bombyx mori contractile (cot) behavioral mutant with thermo-sensitive seizures phenotype. At elevated temperatures, the cot mutant exhibit seizures associated with strong contractions, rolling, vomiting, and a temporary lack of movement. We narrowed a region containing cot to ~268 kb by positional cloning and identified the mutant gene as Bmsei which encoded a potassium channel protein. Bmsei was present in both the cell membrane and cytoplasm in wild-type ganglia but faint in cot. Furthermore, Bmsei was markedly decreased upon high temperature treatment in cot mutant. With the RNAi method and injecting potassium channel blockers, the wild type silkworm was induced the cot phenotype. These results demonstrated that Bmsei was responsible for the cot mutant phenotype and played an important role in thermoprotection in silkworm. Meanwhile, comparative proteomic approach was used to investigate the proteomic differences. The results showed that the protein of Hsp-1 and Tn1 were significantly decreased and increased on protein level in cot mutant after thermo-stimulus, respectively. Our data provide insights into the mechanism of thermoprotection in insect. As cot phenotype closely resembles human epilepsy, cot might be a potential model for the mechanism of epilepsy in future.

Functional Loss of Bmsei Causes Thermosensitive Epilepsy in Contractile Mutant Silkworm, Bombyx mori

PubMed Central

Nie, Hongyi; Cheng, Tingcai; Huang, Xiaofeng; Zhou, Mengting; Zhang, Yinxia; Dai, Fangyin; Mita, Kazuei; Xia, Qingyou; Liu, Chun

2015-01-01

The thermoprotective mechanisms of insects remain largely unknown. We reported the Bombyx mori contractile (cot) behavioral mutant with thermo-sensitive seizures phenotype. At elevated temperatures, the cot mutant exhibit seizures associated with strong contractions, rolling, vomiting, and a temporary lack of movement. We narrowed a region containing cot to ~268 kb by positional cloning and identified the mutant gene as Bmsei which encoded a potassium channel protein. Bmsei was present in both the cell membrane and cytoplasm in wild-type ganglia but faint in cot. Furthermore, Bmsei was markedly decreased upon high temperature treatment in cot mutant. With the RNAi method and injecting potassium channel blockers, the wild type silkworm was induced the cot phenotype. These results demonstrated that Bmsei was responsible for the cot mutant phenotype and played an important role in thermoprotection in silkworm. Meanwhile, comparative proteomic approach was used to investigate the proteomic differences. The results showed that the protein of Hsp-1 and Tn1 were significantly decreased and increased on protein level in cot mutant after thermo-stimulus, respectively. Our data provide insights into the mechanism of thermoprotection in insect. As cot phenotype closely resembles human epilepsy, cot might be a potential model for the mechanism of epilepsy in future. PMID:26198671

Integrated in silico and biological validation of the blocking effect of Cot-1 DNA on Microarray-CGH.

PubMed

Kang, Seung-Hui; Park, Chan Hee; Jeung, Hei Cheul; Kim, Ki-Yeol; Rha, Sun Young; Chung, Hyun Cheol

2007-06-01

In array-CGH, various factors may act as variables influencing the result of experiments. Among them, Cot-1 DNA, which has been used as a repetitive sequence-blocking agent, may become an artifact-inducing factor in BAC array-CGH. To identify the effect of Cot-1 DNA on Microarray-CGH experiments, Cot-1 DNA was labeled directly and Microarray-CGH experiments were performed. The results confirmed that probes which hybridized more completely with Cot-1 DNA had a higher sequence similarity to the Alu element. Further, in the sex-mismatched Microarray-CGH experiments, the variation and intensity in the fluorescent signal were reduced in the high intensity probe group in which probes were better hybridized with Cot-1 DNA. Otherwise, those of the low intensity probe group showed no alterations regardless of Cot-1 DNA. These results confirmed by in silico methods that Cot-1 DNA could block repetitive sequences in gDNA and probes. In addition, it was confirmed biologically that the blocking effect of Cot-1 DNA could be presented via its repetitive sequences, especially Alu elements. Thus, in contrast to BAC-array CGH, the use of Cot-1 DNA is advantageous in controlling experimental variation in Microarray-CGH.

Towards an exact correlated orbital theory for electrons

NASA Astrophysics Data System (ADS)

Bartlett, Rodney J.

2009-12-01

The formal and computational attraction of effective one-particle theories like Hartree-Fock and density functional theory raise the question of how far such approaches can be taken to offer exact results for selected properties of electrons in atoms, molecules, and solids. Some properties can be exactly described within an effective one-particle theory, like principal ionization potentials and electron affinities. This fact can be used to develop equations for a correlated orbital theory (COT) that guarantees a correct one-particle energy spectrum. They are built upon a coupled-cluster based frequency independent self-energy operator presented here, which distinguishes the approach from Dyson theory. The COT also offers an alternative to Kohn-Sham density functional theory (DFT), whose objective is to represent the electronic density exactly as a single determinant, while paying less attention to the energy spectrum. For any estimate of two-electron terms COT offers a litmus test of its accuracy for principal Ip's and Ea's. This feature for approximating the COT equations is illustrated numerically.

UWE-3, in-orbit performance and lessons learned of a modular and flexible satellite bus for future pico-satellite formations

NASA Astrophysics Data System (ADS)

Busch, S.; Bangert, P.; Dombrovski, S.; Schilling, K.

2015-12-01

Formations of small satellites offer promising perspectives due to improved temporal and spatial coverage and resolution at reasonable costs. The UWE-program addresses in-orbit demonstrations of key technologies to enable formations of cooperating distributed spacecraft at pico-satellite level. In this context, the CubeSat UWE-3 addresses experiments for evaluation of real-time attitude determination and control. UWE-3 introduces also a modular and flexible pico-satellite bus as a robust and extensible base for future missions. Technical objective was a very low power consumption of the COTS-based system, nevertheless providing a robust performance of this miniature satellite by advanced microprocessor redundancy and fault detection, identification and recovery software. This contribution addresses the UWE-3 design and mission results with emphasis on the operational experiences of the attitude determination and control system.

A 10-yr Analysis of Chronic Pelvic Pain and Chronic Opioid Therapy in the Women Veteran Population.

PubMed

Cichowski, Sara B; Rogers, Rebecca G; Komesu, Yuko; Murata, Erin; Qualls, Clifford; Murata, Allison; Murata, Glen

2018-05-18

Chronic pelvic pain (CPP) affects an estimated 30% of women Veterans. Previous research shows high rates of narcotic abuse in the women Veteran population. Narcotics are not recommended for the treatment of CPP. Understanding how CPP impacts narcotic prescribing in the women Veteran population is critical to addressing the public health crisis of opioid abuse. Our objective was to compare chronic opioid therapy (COT) prescribed 5 yr prior to and following CPP diagnosis and to identify predictors of COT as well as adverse events associated with COT. We choose to look at 10 yr of data because we thought this time period would provide unique insight into the longitudinal associations of CPP and COT and was available in the database. Women with non-cancer CPP were included for analyses from the Veteran's Affairs Corporate Database Warehouse. COT was defined as 90 d of opiates/calendar year for each of the 5 yr proceeding and following the diagnosis of CPP. Patient characteristics and potential variables influencing COT were collected. We compared baseline demographics between the women who received COT to the women who did not receive COT to find additional demographic predictors of COT in association with CPP. Multivariable analysis identified predictors of COT in this population of women with CPP. We utilized an interrupted time series analysis to understand the impact of the diagnosis of CPP on COT. A total of 49,601 women met inclusion criteria with an average age of 40.1 ± 11.5 yr; 37.3% self-characterized as being a racial minority and 24% had a history of military sexual trauma. Chronic use increased significantly (p < 0.001) in the 5 yr preceding the diagnosis of CPP from 6.3% (n = 3124) of women at time -5 to 13.6% (n = 6746) at time 0. In the first year following the diagnosis of CPP, 16.8% (n = 8,333) of women with CPP met the criteria for COT (p < 0.001) and 15% (n = 7440) of women with CPP remained in the COT group for the remaining 5 yr following the diagnosis. On average women in the COT group had 250-292 d of opioids/year. When comparing women who received chronic narcotics following the diagnosis of CPP versus those who did not receive chronic narcotics, women who received COT were older, more likely to smoke and more frequently diagnosed with other pain conditions such as back pain, headaches, and fibromyalgia. (All p < 0.001). In the multivariable model, predictors of COT following CPP diagnosis included prior COT (OR = 10.0 (95% CI 9.4, 10.6), a positive history of military sexual trauma, smoking, and other chronic pain conditions. The distinct pattern of prescribing shown in this cohort may mean COT is prescribed for CPP and this prescribing pattern contributes to the adverse events associated with COT. As COT is not recommended for CPP, physicians need more education on the therapies available to help CPP patients.

Lithium-Ion Batteries Based on Commercial Cells: Past, Present and Future

NASA Astrophysics Data System (ADS)

Spurrett, R.; Simmons, N.; Pearson, C.; Dudley, G.

2008-09-01

This paper describes the very early development and applications of Lithium-ion battery technology to space missions. This development was performed by ABSL (then AEA Technology) in collaboration with the European Space Agency (ESA) and the British National Space Centre (BNSC).A key factor in the establishment of lithium-ion as the Space battery chemistry of choice was the availability of high-quality commercial off-the-shelf (COTS) cells that enabled short experimental missions to be flown with confidence. Over time it was realized that the application of COTS cells was wider than originally thought, as the cycle life and uniformity of one particular commercial cell enabled larger batteries and longer mission to be addressed.This paper documents the historical development of this ground-breaking European innovation and a vision of the role of the COTS based batteries in future missions.

Goal Structured Notation in a Radiation Hardening Safety Case for COTS-Based Spacecraft

NASA Technical Reports Server (NTRS)

Witulski, Arthur; Austin, Rebekah; Reed, Robert; Karsai, Gabor; Mahadevan, Nag; Sierawski, Brian; Evans, John; LaBel, Ken

2016-01-01

A systematic approach is presented to constructing a radiation assurance case using Goal Structured Notation (GSN) for spacecraft containing COTS parts. The GSN paradigm is applied to an SRAM single-event upset experiment board designed to fly on a CubeSat November 2016. Construction of a radiation assurance case without use of hardened parts or extensive radiation testing is discussed.

Space Biology Initiative. Trade Studies, volume 2

NASA Technical Reports Server (NTRS)

1989-01-01

The six studies which are the subjects of this report are entitled: Design Modularity and Commonality; Modification of Existing Hardware (COTS) vs. New Hardware Build Cost Analysis; Automation Cost vs. Crew Utilization; Hardware Miniaturization versus Cost; Space Station Freedom/Spacelab Modules Compatibility vs. Cost; and Prototype Utilization in the Development of Space Hardware. The product of these six studies was intended to provide a knowledge base and methodology that enables equipment produced for the Space Biology Initiative program to meet specific design and functional requirements in the most efficient and cost effective form consistent with overall mission integration parameters. Each study promulgates rules of thumb, formulas, and matrices that serves as a handbook for the use and guidance of designers and engineers in design, development, and procurement of Space Biology Initiative (SBI) hardware and software.

Space Biology Initiative. Trade Studies, volume 1

NASA Technical Reports Server (NTRS)

1989-01-01

The six studies which are addressed are entitled: Design Modularity and Commonality; Modification of Existing Hardware (COTS) vs. New Hardware Build Cost Analysis; Automation Cost vs. Crew Utilization; Hardware Miniaturization versus Cost; Space Station Freedom/Spacelab Modules Compatibility vs. Cost; and Prototype Utilization in the Development of Space Hardware. The product of these six studies was intended to provide a knowledge base and methodology that enables equipment produced for the Space Biology Initiative program to meet specific design and functional requirements in the most efficient and cost effective form consistent with overall mission integration parameters. Each study promulgates rules of thumb, formulas, and matrices that serves has a handbook for the use and guidance of designers and engineers in design, development, and procurement of Space Biology Initiative (SBI) hardware and software.

Prevalence of prescription opioid use disorder among chronic opioid therapy patients after health plan opioid dose and risk reduction initiatives.

PubMed

Von Korff, Michael; Walker, Rod L; Saunders, Kathleen; Shortreed, Susan M; Thakral, Manu; Parchman, Michael; Hansen, Ryan N; Ludman, Evette; Sherman, Karen J; Dublin, Sascha

2017-08-01

No studies have assessed the comparative effectiveness of guideline-recommended interventions to reduce risk of prescription opioid use disorder among chronic opioid therapy (COT) patients. We compared the prevalence of prescription opioid use disorder among COT patients from intervention clinics that had implemented opioid dose and risk reduction initiatives for more than 4 years relative to control clinics that had not. After a healthcare system in Washington State implemented interventions to reduce opioid dose and risks, we surveyed 1588 adult primary care COT patients to compare the prevalence of prescription opioid use disorder among COT patients from the intervention and control clinics. Intervention clinics managed COT patients at lower COT doses and with more consistent use of risk reduction practices. Control clinics cared for similar COT patients but prescribed higher opioid doses and used COT risk reduction practices inconsistently. Prescription opioid use disorder was assessed with the Psychiatric Research Interview for Substance and Mental Disorders. The prevalence of prescription opioid use disorder was 21.5% (95% CI=18.9% to 24.4%) among COT patients in the intervention clinics and 23.9% (95% CI=20.5% to 27.6%) among COT patients in the control clinics. The adjusted relative risk of prescription opioid use disorder was 1.08 (95% CI=0.89, 1.32) among the control clinic patients relative to the intervention clinic patients. Long-term implementation of opioid dose and risk reduction initiatives was not associated with lower rates of prescription opioid use disorder among prevalent COT patients. Extreme caution should be exercised by clinicians considering COT for patients with chronic non-cancer pain until benefits of this treatment and attendant risks are clarified. Copyright © 2017 Elsevier B.V. All rights reserved.

Involvement of Cot/Tp12 in bone loss during periodontitis.

PubMed

Ohnishi, T; Okamoto, A; Kakimoto, K; Bandow, K; Chiba, N; Matsuguchi, T

2010-02-01

Periodontitis causes resorption of alveolar bone, in which RANKL induces osteoclastogenesis. The binding of lipopolysaccharide to Toll-like receptors causes phosphorylation of Cot/Tp12 to activate the MAPK cascade. Previous in vitro studies showed that Cot/Tp12 was essential for the induction of RANKL expression by lipopolysaccharide. In this study, we examined whether Cot/Tp12 deficiency reduced the progression of alveolar bone loss and osteoclastogenesis during experimental periodontitis. We found that the extent of alveolar bone loss and osteoclastogenesis induced by ligature-induced periodontitis was decreased in Cot/Tp12-deficient mice. In addition, reduction of RANKL expression was observed in periodontal tissues of Cot/Tp12-deficient mice with experimental periodontitis. Furthermore, we found that Cot/Tp12 was involved in the induction of TNF-alpha mRNA expression in gingiva of mice with experimental periodontitis. Our observations suggested that Cot/Tp12 is essential for the progression of alveolar bone loss and osteoclastogenesis in periodontal tissue during experimental periodontitis mediated through increased RANKL expression.

Network Meta-Analysis of Randomized Controlled Trials: Efficacy and Safety of UDCA-Based Therapies in Primary Biliary Cirrhosis

PubMed Central

Zhu, Gui-Qi; Shi, Ke-Qing; Huang, Sha; Huang, Gui-Qian; Lin, Yi-Qian; Zhou, Zhi-Rui; Braddock, Martin; Chen, Yong-Ping; Zheng, Ming-Hua

2015-01-01

Abstract Major ursodeoxycholic acid (UDCA)-based therapies for primary biliary cirrhosis (PBC) include UDCA only, or combined with either methotrexate (MTX), corticosteroids (COT), colchicine (COC), or bezafibrate (BEF). As the optimum treatment regimen is unclear and warrants exploration, we aimed to compare these therapies in terms of patient mortality or liver transplantation (MOLT) and adverse events (AE). PubMed, the Cochrane Library, and Scopus were searched for randomized controlled trials up to August 31, 2014. We estimated the hazard ratios (HRs) for MOLT and odds ratios (ORs) for AE. A sensitivity analysis based on the dose of UDCA was also executed. Thirty-one eligible articles were included. Compared with COT plus UDCA, UDCA (HR 0.38, 95% confidence interval [CI] 0.09–1.39), BEF plus UDCA (HR 0.29, 95% CI 0.02–4.83), COC plus UDCA (HR 0.39, 95% CI 0.07–2.25), MTX plus UDCA (HR 0.28, 95% CI 0.05–1.63), or OBS (HR 0.49, 95% CI 0.11–2.01) all provided an increased risk of MOLT. With respect to drug AE profile, although not differing appreciably, BEF plus UDCA was associated with more AEs compared with UDCA (OR 3.16, 95% CI 0.59–20.67), COT plus UDCA (OR 2.27, 95% CI 0.15–33.36), COC plus UDCA (OR 1.00, 95% CI 0.09–12.16), MTX plus UDCA (OR 2.03, 95% CI 0.23–17.82), or OBS (OR 3.00, 95% CI 0.53–20.75). The results of sensitivity analyses were highly consistent with previous analyses. COT plus UDCA was the optimal UDCA-based regimen for both MOLT and AEs. BEF plus UDCA was most likely to cause AEs, whereas monotherapy with UDCA and coadministriation of COT plus UDCA appeared to be associated with the fewest AEs for PBC treatment. PMID:25789951

Radiation Tolerant, FPGA-Based SmallSat Computer System

NASA Technical Reports Server (NTRS)

LaMeres, Brock J.; Crum, Gary A.; Martinez, Andres; Petro, Andrew

2015-01-01

The Radiation Tolerant, FPGA-based SmallSat Computer System (RadSat) computing platform exploits a commercial off-the-shelf (COTS) Field Programmable Gate Array (FPGA) with real-time partial reconfiguration to provide increased performance, power efficiency and radiation tolerance at a fraction of the cost of existing radiation hardened computing solutions. This technology is ideal for small spacecraft that require state-of-the-art on-board processing in harsh radiation environments but where using radiation hardened processors is cost prohibitive.

Space vehicle field unit and ground station system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Judd, Stephen; Dallmann, Nicholas; Delapp, Jerry

A field unit and ground station may use commercial off-the-shelf (COTS) components and share a common architecture, where differences in functionality are governed by software. The field units and ground stations may be easy to deploy, relatively inexpensive, and be relatively easy to operate. A novel file system may be used where datagrams of a file may be stored across multiple drives and/or devices. The datagrams may be received out of order and reassembled at the receiving device.

Space vehicle field unit and ground station system

DOEpatents

Judd, Stephen; Dallmann, Nicholas; Delapp, Jerry; Proicou, Michael; Seitz, Daniel; Michel, John; Enemark, Donald

2016-10-25

A field unit and ground station may use commercial off-the-shelf (COTS) components and share a common architecture, where differences in functionality are governed by software. The field units and ground stations may be easy to deploy, relatively inexpensive, and be relatively easy to operate. A novel file system may be used where datagrams of a file may be stored across multiple drives and/or devices. The datagrams may be received out of order and reassembled at the receiving device.

Dynamic Detection of Malicious Code in COTS Software

DTIC Science & Technology

2000-04-01

run the following documented hostile applets or ActiveX of these tools work only on mobile code (Java, ActiveX , controls: 16-11 Hostile Applets Tiny...Killer App Exploder Runner ActiveX Check Spy eSafe Protect Desktop 9/9 blocked NB B NB 13/17 blocked NB Surfinshield Online 9/9 blocked NB B B 13/17...Exploder is an ActiveX control top (@). that performs a clean shutdown of your computer. The interface is attractive, although rather complex, as McLain’s

An Automated Tool to Enable the Distributed Operations of Air Force Satellites

DTIC Science & Technology

2002-01-01

workstations, home PCs, PDAs, pagers) over connections with various bandwidths (e.g., dial-up 56k , wireless 9.6k), SERS has different USis to support the...demonstration and evaluation activities, and (3) CERES employs more modem and open ground systems than are currently deployed in the space operations...COTS or custom tools. • Yes, we demonstrated that our software can interface with a modem Air Force ground system (CERES’ COBRA). • We identified new

Application of a COTS Resource Optimization Framework to the SSN Sensor Tasking Domain - Part I: Problem Definition

NASA Astrophysics Data System (ADS)

Tran, T.

With the onset of the SmallSat era, the RSO catalog is expected to see continuing growth in the near future. This presents a significant challenge to the current sensor tasking of the SSN. The Air Force is in need of a sensor tasking system that is robust, efficient, scalable, and able to respond in real-time to interruptive events that can change the tracking requirements of the RSOs. Furthermore, the system must be capable of using processed data from heterogeneous sensors to improve tasking efficiency. The SSN sensor tasking can be regarded as an economic problem of supply and demand: the amount of tracking data needed by each RSO represents the demand side while the SSN sensor tasking represents the supply side. As the number of RSOs to be tracked grows, demand exceeds supply. The decision-maker is faced with the problem of how to allocate resources in the most efficient manner. Braxton recently developed a framework called Multi-Objective Resource Optimization using Genetic Algorithm (MOROUGA) as one of its modern COTS software products. This optimization framework took advantage of the maturing technology of evolutionary computation in the last 15 years. This framework was applied successfully to address the resource allocation of an AFSCN-like problem. In any resource allocation problem, there are five key elements: (1) the resource pool, (2) the tasks using the resources, (3) a set of constraints on the tasks and the resources, (4) the objective functions to be optimized, and (5) the demand levied on the resources. In this paper we explain in detail how the design features of this optimization framework are directly applicable to address the SSN sensor tasking domain. We also discuss our validation effort as well as present the result of the AFSCN resource allocation domain using a prototype based on this optimization framework.

Commercial Orbital Transportation Services (COTS) Demonstrations

NASA Technical Reports Server (NTRS)

Lindenmoyer, Allan

2006-01-01

U.S. space policy directs pursuit of commercial opportunities for providing transportation and other services low Earth orbit and beyond. COTS Project established to implement policy. COTS strategy: Phase 1) Assist industry with system development/demonstrations (COTS Demos); Phase 2) Procure commercial services for ISS logistics support. COTS Demonstrations competition completed in 10 months. Two industry partners selected for funded Space Act Agreements: 1) SpaceX & Rocketplane-Kistler; and 2) Unfunded Space Act Agreements in work with other competitors. COTS budget of $500 M thru 2010, with pay for performance milestone approach. Cargo flight demonstrations planned for 2008 and 2009: Crew flight demonstration options for 2011-2012. Commercial cargo transportation services potentially available as early as 2009-2010. Successful COTS partners may open new space markets and provide reliable, cost effective cargo and crew transportation services, a new era for commercial space.

Phosphorylation of threonine 290 in the activation loop of Tpl2/Cot is necessary but not sufficient for kinase activity.

PubMed

Luciano, Brenda S; Hsu, Sang; Channavajhala, Padma L; Lin, Lih-Ling; Cuozzo, John W

2004-12-10

Cot/Tpl2/MAP3K8 is a serine/threonine kinase known to activate the ERK, p38, and JNK kinase pathways. Studies of Tpl2 knock-out mice reveal a clear defect in tumor necrosis factor-alpha production, although very little detail is known about its regulation and the signaling events involved. In the present study we demonstrated that phosphorylation of Cot was required for its maximal activity as phosphatase treatment of Cot decreased its kinase activity. The Cot sequence contains a conserved threonine at position 290 in the activation loop of the kinase domain. We found that mutation of this residue to alanine eliminated its ability to activate MEK/ERK and NF-kappaB pathways, whereas a phosphomimetic mutation to aspartic acid could rescue the ability to activate MEK. Thr-290 was also required for robust autophosphorylation of Cot. Antibody generated to phospho-Thr-290-Cot recognized both wild-type and kinase-dead Cot, suggesting that phosphorylation of Thr-290 did not occur through autophosphorylation but via another kinase. We showed that Cot was constitutively phosphorylated at Thr-290 in transfected human embryonic kidney 293T cells as well as human monocytes as this residue was phosphorylated in unstimulated and lipopolysaccharide-stimulated cells to the same degree. Treatment with herbimycin A inhibited Cot activity in the MEK/ERK pathway but did not inhibit phosphorylation at Thr-290. Together these results showed that phosphorylation of Cot at Thr-290 is necessary but not sufficient for full kinase activity in the MEK/ERK pathway.

Real-Time On-Board Airborne Demonstration of High-Speed On-Board Data Processing for Science Instruments (HOPS)

NASA Technical Reports Server (NTRS)

Beyon, Jeffrey Y.; Ng, Tak-Kwong; Davis, Mitchell J.; Adams, James K.; Bowen, Stephen C.; Fay, James J.; Hutchinson, Mark A.

2015-01-01

The project called High-Speed On-Board Data Processing for Science Instruments (HOPS) has been funded by NASA Earth Science Technology Office (ESTO) Advanced Information Systems Technology (AIST) program since April, 2012. The HOPS team recently completed two flight campaigns during the summer of 2014 on two different aircrafts with two different science instruments. The first flight campaign was in July, 2014 based at NASA Langley Research Center (LaRC) in Hampton, VA on the NASA's HU-25 aircraft. The science instrument that flew with HOPS was Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) CarbonHawk Experiment Simulator (ACES) funded by NASA's Instrument Incubator Program (IIP). The second campaign was in August, 2014 based at NASA Armstrong Flight Research Center (AFRC) in Palmdale, CA on the NASA's DC-8 aircraft. HOPS flew with the Multifunctional Fiber Laser Lidar (MFLL) instrument developed by Excelis Inc. The goal of the campaigns was to perform an end-to-end demonstration of the capabilities of the HOPS prototype system (HOPS COTS) while running the most computationally intensive part of the ASCENDS algorithm real-time on-board. The comparison of the two flight campaigns and the results of the functionality tests of the HOPS COTS are presented in this paper.

Upgrading Custom Simulink Library Components for Use in Newer Versions of Matlab

NASA Technical Reports Server (NTRS)

Stewart, Camiren L.

2014-01-01

The Spaceport Command and Control System (SCCS) at Kennedy Space Center (KSC) is a control system for monitoring and launching manned launch vehicles. Simulations of ground support equipment (GSE) and the launch vehicle systems are required throughout the life cycle of SCCS to test software, hardware, and procedures to train the launch team. The simulations of the GSE at the launch site in conjunction with off-line processing locations are developed using Simulink, a piece of Commercial Off-The-Shelf (COTS) software. The simulations that are built are then converted into code and ran in a simulation engine called Trick, a Government off-the-shelf (GOTS) piece of software developed by NASA. In the world of hardware and software, it is not uncommon to see the products that are utilized be upgraded and patched or eventually fade away into an obsolete status. In the case of SCCS simulation software, Matlab, a MathWorks product, has released a number of stable versions of Simulink since the deployment of the software on the Development Work Stations in the Linux environment (DWLs). The upgraded versions of Simulink has introduced a number of new tools and resources that, if utilized fully and correctly, will save time and resources during the overall development of the GSE simulation and its correlating documentation. Unfortunately, simply importing the already built simulations into the new Matlab environment will not suffice as it will produce results that may not be expected as they were in the version that is currently being utilized. Thus, an upgrade execution plan was developed and executed to fully upgrade the simulation environment to one of the latest versions of Matlab.

Radiation Test Results on COTS and non-COTS Electronic Devices for NASA-JSC Space Flight Projects

NASA Technical Reports Server (NTRS)

Allums, Kimberly K.; O'Neill, P. M.; Reddell, B. D.; Nguyen, K. V.; Bailey, C. R.

2012-01-01

This presentation reports the results of recent proton and heavy ion Single Event Effect (SEE) testing on a variety of COTS and non-COTs electronic devices and assemblies tested for the Space Shuttle, International Space Station (ISS) and Multi-Purpose Crew Vehicle (MPCV).

Lower Crustal Strength Controls on Melting and Serpentinization at Magma-Poor Margins: Potential Implications for the South Atlantic

NASA Astrophysics Data System (ADS)

Ros, Elena; Pérez-Gussinyé, Marta; Araújo, Mario; Thoaldo Romeiro, Marco; Andrés-Martínez, Miguel; Morgan, Jason P.

2017-12-01

Rifted continental margins may present a predominantly magmatic continent-ocean transition (COT), or one characterized by large exposures of serpentinized mantle. In this study we use numerical modeling to show the importance of the lower crustal strength in controlling the amount and onset of melting and serpentinization during rifting. We propose that the relative timing between both events controls the nature of the COT. Numerical experiments for half-extension velocities <=10 mm/yr suggest there is a genetic link between margin tectonic style and COT nature that strongly depends on the lower crustal strength. Our results imply that very slow extension velocities (< 5 mm/yr) and a strong lower crust lead to margins characterized by large oceanward dipping faults, strong syn-rift subsidence and abrupt crustal tapering beneath the continental shelf. These margins can be either narrow symmetric or asymmetric and present a COT with exhumed serpentinized mantle underlain by some magmatic products. In contrast, a weak lower crust promotes margins with a gentle crustal tapering, small faults dipping both ocean- and landward and small syn-rift subsidence. Their COT is predominantly magmatic at any ultra-slow extension velocity and perhaps underlain by some serpentinized mantle. These margins can also be either symmetric or asymmetric. Our models predict that magmatic underplating mostly underlies the wide margin at weak asymmetric conjugates, whereas the wide margin is mainly underlain by serpentinized mantle at strong asymmetric margins. Based on this conceptual template, we propose different natures for the COTs in the South Atlantic.

Goal Structuring Notation in a Radiation Hardening Assurance Case for COTS-Based Spacecraft

NASA Technical Reports Server (NTRS)

Witulski, Arthur; Austin, Rebekah; Evans, John; Mahadevan, Nag; Karsai, Gabor; Sierawski, Brian; LaBel, Ken; Reed, Robert; Schrimpf, Ron

2016-01-01

A systematic approach is presented to constructing a radiation assurance case using Goal Structuring Notation (GSN) for spacecraft containing commercial-off-the-shelf (COTS) parts. The GSN paradigm is applied to an SRAM single-event upset experiment board designed to fly on a CubeSat November 2016. Construction of a radiation assurance case without use of hardened parts or extensive radiation testing is discussed.

Cot/Tpl-2 protein kinase as a target for the treatment of inflammatory disease.

PubMed

George, D; Salmeron, A

2009-01-01

Cot/Tpl-2/MAP3K8 is a serine/threonine protein kinase that is essential for lipopolysaccharide (LPS)-induced activation of the MEK/ERK pathway in macrophages as demonstrated in Cot/Tpl-2-deficient mice. Cot/Tpl-2 kinase activation plays an integral role in the production of pro-inflammatory cytokines such as TNF and IL-1beta in this immune cell type. Elevated levels of these cytokines have been clinically implicated as mediators of a number of autoimmune diseases, in particular, the pain and joint destruction of rheumatoid arthritis. By inference, pharmaceutical agents that inhibit Cot/Tpl-2 kinase have the potential to be novel and effective therapies for the treatment of these diseases. This review will describe the physiological regulation and importance of Cot/Tpl-2 in inflammation as well as the landscape of small molecules that have been reported as Cot/Tpl-2 inhibitors.

Use of COTS Batteries on ISS and Shuttle: Payload Safety and Mission Success

NASA Technical Reports Server (NTRS)

Jeevarajan, Judith A.

2004-01-01

Contents: Current program requirements; Challenges with COTS batteries; manned vehicle COTS methodology in use; List of typical flight COTS batteries; Energy content and toxicity; Hazards, failure modes and controls for different battery chemistries; JSC test details; List of incidents from Consumer Protection Safety Commission; Conclusions ans recommendations.

Adaptation and validation of Common Object Token (COT) test into the Sinhalese language.

PubMed

Jeyaraman, Janani; Kumarasinghe, Chameera; Mohamed Rafi, Shabnam Fathima; Mendis, Thirimadura Lakna Amalie; Abdul Rasheed, Fathima Shameema

2016-04-01

This manuscript presents a translation and adaptation of the Common Object Token (COT) test, which assesses speech perception, into the Sinhalese language and an attempt to validate it for use on children with normal hearing (NH) and children with a cochlear implant (CI). Ninety-five children (70 with NH, 25 with a CI) participated in the study. The COT test was translated, back-translated, and evaluated by a team of experts until the Sinhalese translation was deemed acceptable. Data of Sinhalese children with NH and values of children with a CI were analysed. Internal reliability and consistency of the COT total score were determined. Lastly, a quick version of the COT test was created. The total mean scores and subtest mean scores improved with age for children with NH. For children with a CI, a strong relationship between the COT total score and device experience, i.e. hearing age, was found. A Quick Sinhalese COT test version, suitable for children with a CI, could be created from Subtests 2, 3, and 4. The Sinhalese COT test is valid for assessing the age-related development of speech perception and identification skills of children with NH. Results suggest that the COT is valid for use in children with a CI. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Indirect effects of ocean acidification drive feeding and growth of juvenile crown-of-thorns starfish, Acanthaster planci.

PubMed

Kamya, Pamela Z; Byrne, Maria; Mos, Benjamin; Hall, Lauren; Dworjanyn, Symon A

2017-06-14

The indirect effects of changing climate in modulating trophic interactions can be as important as the direct effects of climate stressors on consumers. The success of the herbivorous juvenile stage of the crown-of-thorns starfish (COTS), Acanthaster planci, may be affected by the impacts of ocean conditions on its crustose coralline algal (CCA) food. To partition the direct effects of near future ocean acidification on juvenile COTS and indirect effects through changes in their CCA food, COTS were grown in three pH T levels (7.9, 7.8, 7.6) and fed CCA grown at similar pH levels. Consumption of CCA by COTS was bolstered when the COTS were grown in low pH and when they were fed CCA grown in low pH regardless of the pH in which the COTS were reared. COTS fed CCA grown at pH 7.6 grew fastest, but the pH/ p CO 2 that the COTS were reared in had no direct effect on growth. Ocean acidification conditions decreased the C : N ratio and carbonate levels in the CCA. Bolstered growth in COTS may be driven by enhanced palatability, increased nutritive state and reduced defences of their CCA food. These results indicate that near future acidification will increase the success of early juvenile COTS and boost recruitment into the coral-eating life stage. © 2017 The Author(s).

Multidimensional Processing and Visual Rendering of Complex 3D Biomedical Images

NASA Technical Reports Server (NTRS)

Sams, Clarence F.

2016-01-01

The proposed technology uses advanced image analysis techniques to maximize the resolution and utility of medical imaging methods being used during spaceflight. We utilize COTS technology for medical imaging, but our applications require higher resolution assessment of the medical images than is routinely applied with nominal system software. By leveraging advanced data reduction and multidimensional imaging techniques utilized in analysis of Planetary Sciences and Cell Biology imaging, it is possible to significantly increase the information extracted from the onboard biomedical imaging systems. Year 1 focused on application of these techniques to the ocular images collected on ground test subjects and ISS crewmembers. Focus was on the choroidal vasculature and the structure of the optic disc. Methods allowed for increased resolution and quantitation of structural changes enabling detailed assessment of progression over time. These techniques enhance the monitoring and evaluation of crew vision issues during space flight.

Creation of digital contours that approach the characteristics of cartographic contours

USGS Publications Warehouse

Tyler, Dean J.; Greenlee, Susan K.

2012-01-01

The capability to easily create digital contours using commercial off-the-shelf (COTS) software has existed for decades. Out-of-the-box raw contours are suitable for many scientific applications without pre- or post-processing; however, cartographic applications typically require additional improvements. For example, raw contours generally require smoothing before placement on a map. Cartographic contours must also conform to certain spatial/logical rules; for example, contours may not cross waterbodies. The objective was to create contours that match as closely as possible the cartographic contours produced by manual methods on the 1:24,000-scale, 7.5-minute Topographic Map series. This report outlines the basic approach, describes a variety of problems that were encountered, and discusses solutions. Many of the challenges described herein were the result of imperfect input raster elevation data and the requirement to have the contours integrated with hydrographic features from the National Hydrography Dataset (NHD).

Sterile inflammation in acetaminophen-induced liver injury is mediated by Cot/tpl2.

PubMed

Sanz-Garcia, Carlos; Ferrer-Mayorga, Gemma; González-Rodríguez, Águeda; Valverde, Angela M; Martín-Duce, Antonio; Velasco-Martín, Juan P; Regadera, Javier; Fernández, Margarita; Alemany, Susana

2013-05-24

Cot/tpl2 (MAP3K8) activates MKK1/2-Erk1/2 following stimulation of the Toll-like/IL-1 receptor superfamily. Here, we investigated the role of Cot/tpl2 in sterile inflammation and drug-induced liver toxicity. Cot/tpl2 KO mice exhibited reduced hepatic injury after acetaminophen challenge, as evidenced by decreased serum levels of both alanine and aspartate aminotransferases, decreased hepatic necrosis, and increased survival relative to Wt mice. Serum levels of both alanine and aspartate aminotransferases were also lower after intraperitoneal injection of acetaminophen in mice expressing an inactive form of Cot/tpl2 compared with Wt mice, suggesting that Cot/tpl2 activity contributes to acetaminophen-induced liver injury. Furthermore, Cot/tpl2 deficiency reduced neutrophil and macrophage infiltration in the liver of mice treated with acetaminophen, as well as their hepatic and systemic levels of IL-1α. Intraperitoneal injection of damage-associated molecular patterns from necrotic hepatocytes also impaired the recruitment of leukocytes and decreased the levels of several cytokines in the peritoneal cavity in Cot/tpl2 KO mice compared with Wt counterparts. Moreover, similar activation profiles of intracellular pathways were observed in Wt macrophages stimulated with Wt or Cot/tpl2 KO damage-associated molecular patterns. However, upon stimulation with damage-associated molecular patterns, the activation of Erk1/2 and JNK was deficient in Cot/tpl2 KO macrophages compared with their Wt counterparts; an effect accompanied by weaker release of several cytokines, including IL-1α, an important component in the development of sterile inflammation. Taken together, these findings indicate that Cot/tpl2 contributes to acetaminophen-induced liver injury, providing some insight into the underlying molecular mechanisms.

Functional roles of Cot/Tpl2 in mast cell responses to lipopolysaccharide and FcεRI-clustering.

PubMed

Chiba, Norika; Kakimoto, Kyoko; Masuda, Akio; Matsuguchi, Tetsuya

2010-11-05

Cot/Tpl2, a member of MAP kinase kinase kinase (MAPKKK), is indispensable for the ERK activation, as well as the production of TNF-α, IL-1β, IL-23, and PGE(2) in lipopolysaccharide (LPS)-stimulated macrophages. However, the expression and the functional roles of Cot/Tpl2 in mast cells have not been elucidated. The administration of LPS impairs allergic airway inflammation in a mast cell-dependent manner, and LPS stimulates mast cells to produce not only pro-inflammatory cytokines, such as IL-6 and TNF-α, but also Th2-type cytokines, such as IL-5, IL-10 and IL-13. Here, we examine the role of Cot/Tpl2 by using bone marrow-derived mast cells (BMMCs) from cot/tpl2 gene-deficient mice. Phosphorylation of ERKs was significantly decreased, whereas that of JNKs and p38 kinase was normal in LPS-stimulated cot/tpl2(-/-) BMMCs compared with wild-type counterparts. LPS-induced mRNA increase was significantly impaired for IL-5, IL-10, IL-13, and TNF-α, but was normal for IL-6, in cot/tpl2(-/-) BMMCs. On the other hand, degranulation by FcεRI-clustering from cot/tpl2(-/-) BMMCs was significantly enhanced compared with the WT control. Although the phosphorylation of ERKs and p38 kinase by FcεRI-clustering was similar in WT and cot/tpl2(-/-) BMMCs, the phosphorylation of Syk was significantly enhanced in cot/tpl2(-/-) BMMCs, which seemed to be due to the increased protein concentration of Syk. These results imply the functional importance of Cot/Tpl2 in mast cells during the course of allergic diseases such as asthma. Copyright © 2010 Elsevier Inc. All rights reserved.

Sterile Inflammation in Acetaminophen-induced Liver Injury Is Mediated by Cot/tpl2*

PubMed Central

Sanz-Garcia, Carlos; Ferrer-Mayorga, Gemma; González-Rodríguez, Águeda; Valverde, Ángela M.; Martín-Duce, Antonio; Velasco-Martín, Juan P.; Regadera, Javier; Fernández, Margarita; Alemany, Susana

2013-01-01

Cot/tpl2 (MAP3K8) activates MKK1/2-Erk1/2 following stimulation of the Toll-like/IL-1 receptor superfamily. Here, we investigated the role of Cot/tpl2 in sterile inflammation and drug-induced liver toxicity. Cot/tpl2 KO mice exhibited reduced hepatic injury after acetaminophen challenge, as evidenced by decreased serum levels of both alanine and aspartate aminotransferases, decreased hepatic necrosis, and increased survival relative to Wt mice. Serum levels of both alanine and aspartate aminotransferases were also lower after intraperitoneal injection of acetaminophen in mice expressing an inactive form of Cot/tpl2 compared with Wt mice, suggesting that Cot/tpl2 activity contributes to acetaminophen-induced liver injury. Furthermore, Cot/tpl2 deficiency reduced neutrophil and macrophage infiltration in the liver of mice treated with acetaminophen, as well as their hepatic and systemic levels of IL-1α. Intraperitoneal injection of damage-associated molecular patterns from necrotic hepatocytes also impaired the recruitment of leukocytes and decreased the levels of several cytokines in the peritoneal cavity in Cot/tpl2 KO mice compared with Wt counterparts. Moreover, similar activation profiles of intracellular pathways were observed in Wt macrophages stimulated with Wt or Cot/tpl2 KO damage-associated molecular patterns. However, upon stimulation with damage-associated molecular patterns, the activation of Erk1/2 and JNK was deficient in Cot/tpl2 KO macrophages compared with their Wt counterparts; an effect accompanied by weaker release of several cytokines, including IL-1α, an important component in the development of sterile inflammation. Taken together, these findings indicate that Cot/tpl2 contributes to acetaminophen-induced liver injury, providing some insight into the underlying molecular mechanisms. PMID:23572518

Transitioning to Intel-based Linux Servers in the Payload Operations Integration Center

NASA Technical Reports Server (NTRS)

Guillebeau, P. L.

2004-01-01

The MSFC Payload Operations Integration Center (POIC) is the focal point for International Space Station (ISS) payload operations. The POIC contains the facilities, hardware, software and communication interface necessary to support payload operations. ISS ground system support for processing and display of real-time spacecraft and telemetry and command data has been operational for several years. The hardware components were reaching end of life and vendor costs were increasing while ISS budgets were becoming severely constrained. Therefore it has been necessary to migrate the Unix portions of our ground systems to commodity priced Intel-based Linux servers. hardware architecture including networks, data storage, and highly available resources. This paper will concentrate on the Linux migration implementation for the software portion of our ground system. The migration began with 3.5 million lines of code running on Unix platforms with separate servers for telemetry, command, Payload information management systems, web, system control, remote server interface and databases. The Intel-based system is scheduled to be available for initial operational use by August 2004 The overall migration to Intel-based Linux servers in the control center involves changes to the This paper will address the Linux migration study approach including the proof of concept, criticality of customer buy-in and importance of beginning with POSlX compliant code. It will focus on the development approach explaining the software lifecycle. Other aspects of development will be covered including phased implementation, interim milestones and metrics measurements and reporting mechanisms. This paper will also address the testing approach covering all levels of testing including development, development integration, IV&V, user beta testing and acceptance testing. Test results including performance numbers compared with Unix servers will be included. need for a smooth transition while maintaining real-time support. An important aspect of the paper will involve challenges and lessons learned. product compatibility, implications of phasing decisions and tracking of dependencies, particularly non- software dependencies. The paper will also discuss scheduling challenges providing real-time flight support during the migration and the requirement to incorporate in the migration changes being made simultaneously for flight support. This paper will also address the deployment approach including user involvement in testing and the , This includes COTS product compatibility, implications of phasing decisions and tracking of dependencies, particularly non- software dependencies. The paper will also discuss scheduling challenges providing real-time flight support during the migration and the requirement to incorporate in the migration changes being made simultaneously for flight support.

Electronic Packaging for Space Applications Workshop 1999

NASA Technical Reports Server (NTRS)

Sandor, Mike; Agarwal, Shri; Villegas, Enrique

1999-01-01

This paper presents viewgraphs on the Commercial Off-The-Shelf (COTS) Program. The topics include: 1) Advocacy for COTS; 2) MARS01 Program/Requirements; 3) MARS01 COTS Screening Flow; 4) Test Results-Electrical, C-Sam, Burn-In; 5) Value Added Analysis (Risk Reduction); 6) Value Added Analysis (Cost); 7) Impact of COTS ++ Screening and 8) Summary.

48 CFR 22.1803 - Contract clause.

Code of Federal Regulations, 2010 CFR

2010-10-01

... COTS items, but for minor modifications (as defined at paragraph (3)(ii) of the definition of “commercial item” at 2.101); (3) Items that would be COTS items if they were not bulk cargo; or (4) Commercial services that are— (i) Part of the purchase of a COTS item (or an item that would be a COTS item, but for...

75 FR 77739 - Federal Acquisition Regulation; Uniform Suspension and Debarment Requirement

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-13

...-the-shelf (COTS) items; and In the case of commercial items, such term includes only the first-tier subcontracts. This has the effect, except for COTS items, of expanding the requirement of 2455(a), which states... for COTS items; subcontractors for COTS items will not be required to disclose to the prime contractor...

Timeliness of Care Planning upon Initiation of Chronic Opioid Therapy for Chronic Pain.

PubMed

Von Korff, Michael; Turner, Judith A; Shortreed, Susan M; Saunders, Kathleen; Rosenberg, Dori; Thielke, Stephen; LeResche, Linda

2016-03-01

Chronic opioid therapy (COT) guidelines recommend developing a COT care plan at the initiation of COT. Assess the timeliness of care planning upon initiation of COT. Observational cohort study in a setting incentivizing and tracking documentation of COT care plans in electronic health records (EHRs). Study participants (N = 896) were aged 45 years or older, had initiated an episode of opioid use within the prior 6 months, and reported regular use of prescription analgesics when screened for a baseline interview about 3 months after an index opioid prescription MEASURES: A timely care plan was defined by an EHR documented care plan prior to or within 4 months after the index opioid prescription. Among COT initiators, 30% had a timely COT care plan documented in the EHR within 4 months following index prescription, while 51% had a documented COT care plan within 12 months following index prescription. Among those interviewed at 1 year follow-up (N = 735), 252 (34.2%) reported opioid use on 7 or more days in the prior 2 weeks. Less than half (45.6%) of the 252 individuals who sustained regular opioid use at 1 year had predicted at baseline that it was somewhat, very, or extremely likely they would be using opioids regularly in 1 year. Patients initiating COT were unlikely to have timely COT care plans. Many who sustained regular opioid use at 1 year had not anticipated using opioids long term. © 2015 American Academy of Pain Medicine. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Evaluation of Health Plan Interventions to Influence Chronic Opioid Therapy Prescribing

PubMed Central

Saunders, Kathleen; Shortreed, Susan; Thielke, Stephen; Turner, Judith A.; LeResche, Linda; Beck, Randi; Von Korff, Michael

2015-01-01

Objectives Evaluate health plan interventions targeting physician chronic opioid therapy (COT) prescribing. Methods In 2006, Group Health’s (GH) integrated group practice (IGP) initiated diverse interventions targeting COT prescriber norms and practices. In 2010, the IGP implemented a COT guideline, including a mandated online course for physicians managing COT. These interventions were not implemented in GH’s network practices. We compared trends in GH-IGP and network practices for 2006–12 in the percent of patients receiving COT and their opioid dose. We compared physician beliefs before versus after the mandated course and pre- to post-course changes in COT dosing for IGP physicians who took the course. Results From 2006 to 2012, mean (SE) daily opioid dose among IGP COT patients (intervention setting) declined from 74.1 (1.9) mg. morphine equivalent dose (MED) to 48.3 (1.0) mg. MED. Dose changes among GH network COT patients (control setting) were modest—88.2 (5.0) mg. MED in 2006 to 75.7 (2.3) mg. MED in 2012. Among physicians taking the mandated course in 2011, we observed pre- to post-course changes toward more conservative opioid prescribing beliefs. However, COT dosing trends did not change pre- to post-course. Discussion Following initiatives implemented to alter physician prescribing practices and norms, mean opioid dose prescribed to COT patients declined more in intervention than control practices. Physicians reported more conservative beliefs regarding opioid prescribing immediately after completing an online course in 2011, but the course was not associated with additional reductions in mean daily opioid dose prescribed by physicians completing the course. PMID:25621426

Expression of the Tpl2/Cot oncogene in human T-cell neoplasias.

PubMed

Christoforidou, Anna V; Papadaki, Helen A; Margioris, Andrew N; Eliopoulos, George D; Tsatsanis, Christos

2004-12-03

Tpl2/Cot oncogene has been identified in murine T-cell lymphomas as a target of MoMuLV insertion. Animal and tissue culture studies have shown that Tpl2/Cot is involved in interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) production by T-cells contributing to T-cell proliferation. In the present report we examined a series of 12 adult patients with various T-cell malignancies, all with predominant leukemic expression in the periphery, for the expression of Tpl2/Cot oncogene in order to determine a possible involvement of Tpl2/Cot in the pathogenesis of these neoplasms. Our results showed that Tpl2/Cot was overexpressed in all four patients with Large Granular Lymphocyte proliferative disorders (LGL-PDs) but in none of the remaining eight patients with other T-cell neoplasias. Interestingly, three of the LGL-PD patients displayed neutropenia, one in association with sarcoidosis. Serum TNF-alpha levels were increased in all Tpl2/Cot overexpressing patients while serum IL-2 was undetectable in all subjects studied. Genomic DNA analysis revealed no DNA amplification at the Tpl2/Cot locus in any of the samples analyzed. We conclude that Tpl2/Cot, a gene extensively studied in animal and tissue culture T-cell models may be also involved in the development of human LGL-PD and may have a role in the pathogenesis of immune manifestations associated with these diseases. This is the first report implicating Tpl2/Cot in human T-cell neoplasias and provides a novel molecular event in the development of LGL-PDs.

Measurement Comparisons Towards Improving the Understanding of Aerosol-Cloud Processing

NASA Astrophysics Data System (ADS)

Noble, Stephen R.

Cloud processing of aerosol is an aerosol-cloud interaction that is not heavily researched but could have implications on climate. The three types of cloud processing are chemical processing, collision and coalescence processing, and Brownian capture of interstitial particles. All types improve cloud condensation nuclei (CCN) in size or hygroscopicity (kappa). These improved CCN affect subsequent clouds. This dissertation focuses on measurement comparisons to improve our observations and understanding of aerosol-cloud processing. Particle size distributions measured at the continental Southern Great Plains (SGP) site were compared with ground based measurements of cloud fraction (CF) and cloud base altitude (CBA). Particle size distributions were described by a new objective shape parameter to define bimodality rather than an old subjective one. Cloudy conditions at SGP were found to be correlated with lagged shape parameter. Horizontal wind speed and regional CF explained 42%+ of this lag time. Many of these surface particle size distributions were influenced by aerosol-cloud processing. Thus, cloud processing may be more widespread with more implications than previously thought. Particle size distributions measured during two aircraft field campaigns (MArine Stratus/stratocumulus Experiment; MASE; and Ice in Cloud Experiment-Tropical; ICE-T) were compared to CCN distributions. Tuning particle size to critical supersaturation revealed hygroscopicity expressed as ? when the distributions were overlain. Distributions near cumulus clouds (ICE-T) had a higher frequency of the same ?s (48% in ICE-T to 42% in MASE) between the accumulation (processed) and Aitken (unprocessed) modes. This suggested physical processing domination in ICE-T. More MASE (stratus cloud) kappa differences between modes pointed to chemical cloud processing. Chemistry measurements made in MASE showed increases in sulfates and nitrates with distributions that were more processed. This supported chemical cloud processing in MASE. This new method to determine kappa provides the needed information without interrupting ambient measurements. MODIS derived cloud optical thickness (COT), cloud liquid water path (LWP), and cloud effective radius (re) were compared to the same in situ derived variables from cloud probe measurements of two stratus/stratocumulus cloud campaigns (MASE and Physics Of Stratocumulus Tops; POST). In situ data were from complete vertical cloud penetrations, while MODIS data were from pixels along the aircraft penetration path. Comparisons were well correlated except that MODIS LWP (14-36%) and re (20-30%) were biased high. The LWP bias was from re bias and was not improved by using the vertically stratified assumption. MODIS re bias was almost removed when compared to cloud top maximum in situ re, but, that does not describe re for the full depth of the cloud. COT is validated by in situ COT. High correlations suggest that MODIS variables are useful in self-comparisons such as gradient changes in stratus cloud re during aerosol-cloud processing.

Flight Computer Processing Avionics for Space Station Microgravity Experiments: A Risk Assessment of Commercial Off-the-Shelf Utilization

NASA Technical Reports Server (NTRS)

Estes, Howard; Liggin, Karl; Crawford, Kevin; Humphries, Rick (Technical Monitor)

2001-01-01

NASA/Marshall Space Flight Center (MSFC) is continually looking for ways to reduce the costs and schedule and minimize the technical risks during the development of microgravity programs. One of the more prominent ways to minimize the cost and schedule is to use off-the-shelf hardware (OTS). However, the use of OTS often increases the risk. This paper addresses relevant factors considered during the selection and utilization of commercial off-the-shelf (COTS) flight computer processing equipment for the control of space station microgravity experiments. The paper will also discuss how to minimize the technical risks when using COTS processing hardware. Two microgravity experiments for which the COTS processing equipment is being evaluated for are the Equiaxed Dendritic Solidification Experiment (EDSE) and the Self-diffusion in Liquid Elements (SDLE) experiment. Since MSFC is the lead center for Microgravity research, EDSE and SDLE processor selection will be closely watched by other experiments that are being designed to meet payload carrier requirements. This includes the payload carriers planned for the International Space Station (ISS). The purpose of EDSE is to continue to investigate microstructural evolution of, and thermal interactions between multiple dendrites growing under diffusion controlled conditions. The purpose of SDLE is to determine accurate self-diffusivity data as a function of temperature for liquid elements selected as representative of class-like structures. In 1999 MSFC initiated a Center Director's Discretionary Fund (CDDF) effort to investigate and determine the optimal commercial data bus architecture that could lead to faster, better, and lower cost data acquisition systems for the control of microgravity experiments. As part of this effort various commercial data acquisition systems were acquired and evaluated. This included equipment with various form factors, (3U, 6U, others) and equipment that utilized various bus structures, (VME, PC104, STD bus). This evaluation of hardware was performed in conjunction with a trade study that considered over twenty (20) different factors relevant to the selection of an optimum design approach. These factors included; safety, sizing and timing, radiation hardness and single event upset, power consumption, heat dissipation, size and volume, expected service life, maintainability, heritage, operating systems, requirements for software reuse, availability of compatible interface boards, relative cost, schedule, reliability, EMI/EMC factors, "hot swap" capability, standards for conduction cooling, I/O capabilities, unique carrier requirements and operating system considerations. The approach to evaluate Safety as part of this study included a review of the Preliminary Hazard Analysis (PHA) for each of the experiment designs and a determination of how each hazard could be addressed and eliminated when different processors were selected. This included evaluating various design approaches and trade-offs between fault tolerant designs and fail-safe designs in accordance with NSTS 1700.7B. This will include the results of radiation testing where available. Various operating systems, such as VxWorks, Linux, QNX, and Embedded NT are evaluated and the advantages and disadvantages of their utilization are also addressed. Design implementation strategies for the various operating systems are considered and discussed. This paper presents the results and recommendations from this trade study. Preliminary conclusions from this study are that safety concerns from lack or radiation testing on COTS equipment can be addressed by additional testing and design considerations, the PC104 bus provided adequate I/O for the SDLE and EDSE microgravity experiments, and PC104 bus components offered significant advantages over VME and cPCI for weight and space reductions.

Commercial Off-The-Shelf (COTS) Electronics Reliability for Space Applications

NASA Technical Reports Server (NTRS)

Pellish, Jonathan

2018-01-01

This presentation describes the accelerating use of Commercial off the Shelf (COTS) parts in space applications. Component reliability and threats in the context of the mission, environment, application, and lifetime. Provides overview of traditional approaches applied to COTS parts in flight applications, and shows challenges and potential paths forward for COTS systems in flight applications it's all about data!

The crown-of-thorns starfish genome as a guide for biocontrol of this coral reef pest.

PubMed

Hall, Michael R; Kocot, Kevin M; Baughman, Kenneth W; Fernandez-Valverde, Selene L; Gauthier, Marie E A; Hatleberg, William L; Krishnan, Arunkumar; McDougall, Carmel; Motti, Cherie A; Shoguchi, Eiichi; Wang, Tianfang; Xiang, Xueyan; Zhao, Min; Bose, Utpal; Shinzato, Chuya; Hisata, Kanako; Fujie, Manabu; Kanda, Miyuki; Cummins, Scott F; Satoh, Noriyuki; Degnan, Sandie M; Degnan, Bernard M

2017-04-05

The crown-of-thorns starfish (COTS, the Acanthaster planci species group) is a highly fecund predator of reef-building corals throughout the Indo-Pacific region. COTS population outbreaks cause substantial loss of coral cover, diminishing the integrity and resilience of reef ecosystems. Here we sequenced genomes of COTS from the Great Barrier Reef, Australia and Okinawa, Japan to identify gene products that underlie species-specific communication and could potentially be used in biocontrol strategies. We focused on water-borne chemical plumes released from aggregating COTS, which make the normally sedentary starfish become highly active. Peptide sequences detected in these plumes by mass spectrometry are encoded in the COTS genome and expressed in external tissues. The exoproteome released by aggregating COTS consists largely of signalling factors and hydrolytic enzymes, and includes an expanded and rapidly evolving set of starfish-specific ependymin-related proteins. These secreted proteins may be detected by members of a large family of olfactory-receptor-like G-protein-coupled receptors that are expressed externally, sometimes in a sex-specific manner. This study provides insights into COTS-specific communication that may guide the generation of peptide mimetics for use on reefs with COTS outbreaks.

Cot/Tpl2 regulates IL-23 p19 expression in LPS-stimulated macrophages through ERK activation.

PubMed

Kakimoto, K; Musikacharoen, T; Chiba, N; Bandow, K; Ohnishi, T; Matsuguchi, T

2010-03-01

We have previously reported that a serine/threonine protein kinase, Cot/Tpl2, is a negative regulator of Th1-type immunity through inhibiting IL-12 expression in antigen presenting cells (APCs) stimulated by Toll-like receptor (TLR) ligands. We here show that Cot/Tpl2(-/-) macrophages produce significantly less IL-23, an important regulator of Th17-type response, than the wild-type counterparts in response to lipopolysaccharide (LPS), which is a ligand for TLR4. The decreased IL-23 production in Cot/Tpl2(-/-) macrophages is, at least partly, regulated at the transcriptional level, as the LPS-mediated IL-23 p19 mRNA induction was significantly less in Cot/Tpl2(-/-) macrophages. Chemical inhibition of extracellular signal-regulated kinase (ERK) activity similarly inhibited IL-23 expression in LPS-stimulated wild-type macrophages. As Cot/Tpl2 is an essential upstream component of the ERK activation pathway of LPS, it is suggested that Cot/Tpl2 positively regulates IL-23 expression through ERK activation. These results indicate that Cot/Tpl2 may be involved in balancing Th1/Th17 differentiation by regulating the expression ratio of IL-12 and IL-23 in APCs.

CO2 rebreathing: a possible contributory factor to some cases of sudden infant death?

PubMed

Skadberg, B T; Oterhals, A; Finborud, K; Markestad, T

1995-09-01

Physical and geometrical conditions influencing carbon dioxide (CO2) accumulation near the face of a sleeping infant positioned deep in a cot or pram (open cot shaft) or underneath bedding (closed cot shaft) were investigated. By means of mathematical and data-based simulation, and an experimental rebreathing model, both hypothetical (dry, exhaled air +20 degrees C) and more physiological conditions (heated, humidified exhaled air, room temperature +20 degrees C; with and without pooling of cold air within the shaft) were tested. With exhaled air at +20 degrees C, the CO2 concentration increased to about 10% within 5 min. The increase was faster the smaller the volume, and the smaller the opening of the cot shaft. When expiratory air was heated, the CO2 concentration increased with the same speed as when the shaft was closed, but to only 0.1-0.3% when the shaft was open. Pooling of cold air in the shaft increased CO2 accumulation 70-200 times the concentration in air (to <5.5%) when the shaft was open. Turbulence of the air outside the open shaft reduced the increase in CO2 concentration. The experiments imply that CO2 may accumulate around an infant's head when placed deep in a cot or pram with the bedding and walls creating a narrow, vertical, shaft-like tunnel to the surrounding air. Although the CO2 concentration may theoretically attain dangerous levels in such circumstances, a rapid equilibrium between the air within and outside the cot usually occurs due to convection of the expiratory air and turbulence from drafts, the infant's body movements and breathing. Such factors will largely eliminated any significant rebreathing with the exception of the extreme situation when expired air is contained within a closed space.

Unattended real-time re-establishment of visibility in high dynamic range video and stills

NASA Astrophysics Data System (ADS)

Abidi, B.

2014-05-01

We describe a portable unattended persistent surveillance system that corrects for harsh illumination conditions, where bright sun light creates mixed contrast effects, i.e., heavy shadows and washouts. These effects result in high dynamic range scenes, where illuminance can vary from few luxes to a 6 figure value. When using regular monitors and cameras, such wide span of illuminations can only be visualized if the actual range of values is compressed, leading to the creation of saturated and/or dark noisy areas and a loss of information in these areas. Images containing extreme mixed contrast cannot be fully enhanced from a single exposure, simply because all information is not present in the original data. The active intervention in the acquisition process is required. A software package, capable of integrating multiple types of COTS and custom cameras, ranging from Unmanned Aerial Systems (UAS) data links to digital single-lens reflex cameras (DSLR), is described. Hardware and software are integrated via a novel smart data acquisition algorithm, which communicates to the camera the parameters that would maximize information content in the final processed scene. A fusion mechanism is then applied to the smartly acquired data, resulting in an enhanced scene where information in both dark and bright areas is revealed. Multi-threading and parallel processing are exploited to produce automatic real time full motion corrected video. A novel enhancement algorithm was also devised to process data from legacy and non-controllable cameras. The software accepts and processes pre-recorded sequences and stills, enhances visible, night vision, and Infrared data, and successfully applies to night time and dark scenes. Various user options are available, integrating custom functionalities of the application into intuitive and easy to use graphical interfaces. The ensuing increase in visibility in surveillance video and intelligence imagery will expand the performance and timely decision making of the human analyst, as well as that of unmanned systems performing automatic data exploitation, such as target detection and identification.

Aberrant Expression of COT Is Related to Recurrence of Papillary Thyroid Cancer

PubMed Central

Lee, Jandee; Jeong, Seonhyang; Park, Jae Hyun; Lee, Cho Rok; Ku, Cheol Ryong; Kang, Sang-Wook; Jeong, Jong Ju; Nam, Kee-Hyun; Shin, Dong Yeob; Lee, Eun Jig; Chung, Woong Youn; Jo, Young Suk

2015-01-01

Abstract Aberrant expression of Cancer Osaka Thyroid Oncogene mitogen-activated protein kinase kinase kinase 8 (COT) (MAP3K8) is a driver of resistance to B-RAF inhibition. However, the de novo expression and clinical implications of COT in papillary thyroid cancer (PTC) have not been investigated. The aim of this study is to investigate the expression of A-, B-, C-RAF, and COT in PTC (n = 167) and analyze the clinical implications of aberrant expression of these genes. Quantitative polymerase chain reaction (qPCR) and immunohistochemical staining (IHC) were performed on primary thyroid cancers. Expression of COT was compared with clinicopathological characteristics including recurrence-free survival. Datasets from public repository (NCBI) were subjected to Gene Set Enrichment Analysis (GSEA). qPCR data showed that the relative mRNA expression of A-, B-, C-RAF and COT of PTC were higher than normal tissues (all P < 0.01). In addition, the expression of COT mRNA in PTC showed positive correlation with A- (r = 0.4083, P < 0.001), B- (r = 0.2773, P = 0.0003), and C-RAF (r = 0.5954, P < 0.001). The mRNA expressions of A-, B,- and C-RAF were also correlated with each other (all P < 0.001). In IHC, the staining intensities of B-RAF and COT were higher in PTC than in normal tissue (P < 0.001). Interestingly, moderate-to-strong staining intensities of B-RAF and COT were more frequent in B-RAFV600E-positive PTC (P < 0.001, P = 0.013, respectively). In addition, aberrant expression of COT was related to old age at initial diagnosis (P = 0.045) and higher recurrence rate (P = 0.025). In multivariate analysis, tumor recurrence was persistently associated with moderate-to-strong staining of COT after adjusting for age, sex, extrathyroidal extension, multifocality, T-stage, N-stage, TNM stage, and B-RAFV600E mutation (odds ratio, 4.662; 95% confidence interval 1.066 − 21.609; P = 0.045). Moreover, moderate-to-strong COT expression in PTC was associated with shorter recurrence-free survival (mean follow-up duration, 14.2 ± 4.1 years; P = 0.0403). GSEA indicated that gene sets related to B-RAF-RAS (P < 0.0001, false discovery rate [FDR] q-value = 0.000) and thyroid differentiation (P = 0.048, FDR q-value = 0.05) scores were enriched in lower COT expression group and gene sets such as T-cell receptor signaling pathway and Toll-like receptor signaling pathway are coordinately upregulated in higher COT expression group (both, P < 0.0001, FDR q-value = 0.000). Aberrant expression of A-, B-, and C-RAF, and COT is frequent in PTC; increased expression of COT is correlated with recurrence of PTC. PMID:25674762

Aberrant expression of COT is related to recurrence of papillary thyroid cancer.

PubMed

Lee, Jandee; Jeong, Seonhyang; Park, Jae Hyun; Lee, Cho Rok; Ku, Cheol Ryong; Kang, Sang-Wook; Jeong, Jong Ju; Nam, Kee-Hyun; Shin, Dong Yeob; Lee, Eun Jig; Chung, Woong Youn; Jo, Young Suk

2015-02-01

Aberrant expression of Cancer Osaka Thyroid Oncogene mitogen-activated protein kinase kinase kinase 8 (COT) (MAP3K8) is a driver of resistance to B-RAF inhibition. However, the de novo expression and clinical implications of COT in papillary thyroid cancer (PTC) have not been investigated.The aim of this study is to investigate the expression of A-, B-, C-RAF, and COT in PTC (n = 167) and analyze the clinical implications of aberrant expression of these genes.Quantitative polymerase chain reaction (qPCR) and immunohistochemical staining (IHC) were performed on primary thyroid cancers. Expression of COT was compared with clinicopathological characteristics including recurrence-free survival. Datasets from public repository (NCBI) were subjected to Gene Set Enrichment Analysis (GSEA).qPCR data showed that the relative mRNA expression of A-, B-, C-RAF and COT of PTC were higher than normal tissues (all P < 0.01). In addition, the expression of COT mRNA in PTC showed positive correlation with A- (r = 0.4083, P < 0.001), B- (r = 0.2773, P = 0.0003), and C-RAF (r = 0.5954, P < 0.001). The mRNA expressions of A-, B,- and C-RAF were also correlated with each other (all P < 0.001). In IHC, the staining intensities of B-RAF and COT were higher in PTC than in normal tissue (P < 0.001). Interestingly, moderate-to-strong staining intensities of B-RAF and COT were more frequent in B-RAF-positive PTC (P < 0.001, P = 0.013, respectively). In addition, aberrant expression of COT was related to old age at initial diagnosis (P = 0.045) and higher recurrence rate (P = 0.025). In multivariate analysis, tumor recurrence was persistently associated with moderate-to-strong staining of COT after adjusting for age, sex, extrathyroidal extension, multifocality, T-stage, N-stage, TNM stage, and B-RAF mutation (odds ratio, 4.662; 95% confidence interval 1.066 - 21.609; P = 0.045). Moreover, moderate-to-strong COT expression in PTC was associated with shorter recurrence-free survival (mean follow-up duration, 14.2 ± 4.1 years; P = 0.0403). GSEA indicated that gene sets related to B-RAF-RAS (P < 0.0001, false discovery rate [FDR] q-value = 0.000) and thyroid differentiation (P = 0.048, FDR q-value = 0.05) scores were enriched in lower COT expression group and gene sets such as T-cell receptor signaling pathway and Toll-like receptor signaling pathway are coordinately upregulated in higher COT expression group (both, P < 0.0001, FDR q-value = 0.000).Aberrant expression of A-, B-, and C-RAF, and COT is frequent in PTC; increased expression of COT is correlated with recurrence of PTC.

Lessons Learned Using COTS Electronics for the International Space Station Radiation Environment

NASA Technical Reports Server (NTRS)

Blumer, John H.; Roth, A. (Technical Monitor)

2001-01-01

The mantra of 'Faster, Better, Cheaper' has to a large degree been interpreted as using Commercial Off-the-Shelf (COTS) components and/or circuit boards. One of the first space applications to actually use COTS in space along with radiation performance requirements was the Expedite the Processing of Experiments to Space Station (EXPRESS) Rack program, for the International Space Station (ISS). In order to meet the performance, cost and schedule targets, military grade Versa Module Eurocard (VME) was selected as the baseline design for the main computer, the Rack Interface Controller (RIC). VME was chosen as the computer backplane because of the large variety of military grade boards available, which were designed to meet the military environmental specifications (thermal, shock, vibration, etc.). These boards also have a paper pedigree in regards to components. Since these boards exceeded most ISS environmental requirements, it was reasoned using COTS mid-grade VME boards, as opposed to designing custom boards could save significant time and money. It was recognized up front the radiation environment of ISS, while benign compared to many space flight applications, would be the main challenge to using COTS. Thus in addition to selecting vendors on how well their boards met the usual performance and environmental specifications, the board's parts lists were reviewed on how well they would perform in the ISS radiation environment. However, issues with verifying that the available radiation test data was applicable to the actual part used, vendor part design changes and the fact most parts did not have valid test data soon complicated board and part selection in regards to radiation.

Uav Photogrammetry with Oblique Images: First Analysis on Data Acquisition and Processing

NASA Astrophysics Data System (ADS)

Aicardi, I.; Chiabrando, F.; Grasso, N.; Lingua, A. M.; Noardo, F.; Spanò, A.

2016-06-01

In recent years, many studies revealed the advantages of using airborne oblique images for obtaining improved 3D city models (e.g. including façades and building footprints). Expensive airborne cameras, installed on traditional aerial platforms, usually acquired the data. The purpose of this paper is to evaluate the possibility of acquire and use oblique images for the 3D reconstruction of a historical building, obtained by UAV (Unmanned Aerial Vehicle) and traditional COTS (Commercial Off-the-Shelf) digital cameras (more compact and lighter than generally used devices), for the realization of high-level-of-detail architectural survey. The critical issues of the acquisitions from a common UAV (flight planning strategies, ground control points, check points distribution and measurement, etc.) are described. Another important considered aspect was the evaluation of the possibility to use such systems as low cost methods for obtaining complete information from an aerial point of view in case of emergency problems or, as in the present paper, in the cultural heritage application field. The data processing was realized using SfM-based approach for point cloud generation: different dense image-matching algorithms implemented in some commercial and open source software were tested. The achieved results are analysed and the discrepancies from some reference LiDAR data are computed for a final evaluation. The system was tested on the S. Maria Chapel, a part of the Novalesa Abbey (Italy).

Characterization of an Alkali- and Halide-Resistant Laccase Expressed in E. coli: CotA from Bacillus clausii

PubMed Central

Brander, Søren; Mikkelsen, Jørn D.; Kepp, Kasper P.

2014-01-01

The limitations of fungal laccases at higher pH and salt concentrations have intensified the search for new extremophilic bacterial laccases. We report the cloning, expression, and characterization of the bacterial cotA from Bacillus clausii, a supposed alkalophilic ortholog of cotA from B. subtilis. Both laccases were expressed in E. coli strain BL21(DE3) and characterized fully in parallel for strict benchmarking. We report activity on ABTS, SGZ, DMP, caffeic acid, promazine, phenyl hydrazine, tannic acid, and bilirubin at variable pH. Whereas ABTS, promazine, and phenyl hydrazine activities vs. pH were similar, the activity of B. clausii cotA was shifted upwards by ∼0.5–2 pH units for the simple phenolic substrates DMP, SGZ, and caffeic acid. This shift is not due to substrate affinity (KM) but to pH dependence of catalytic turnover: The kcat of B. clausii cotA was 1 s−1 at pH 6 and 5 s−1 at pH 8 in contrast to 6 s−1 at pH 6 and 2 s−1 at pH 8 for of B. subtilis cotA. Overall, kcat/KM was 10-fold higher for B. subtilis cotA at pHopt. While both proteins were heat activated, activation increased with pH and was larger in cotA from B. clausii. NaCl inhibited activity at acidic pH, but not up to 500–700 mM NaCl in alkaline pH, a further advantage of the alkali regime in laccase applications. The B. clausii cotA had ∼20 minutes half-life at 80°C, less than the ∼50 minutes at 80°C for cotA from B. subtilis. While cotA from B. subtilis had optimal stability at pH∼8, the cotA from B. clausii displayed higher combined salt- and alkali-resistance. This resistance is possibly caused by two substitutions (S427Q and V110E) that could repel anions to reduce anion-copper interactions at the expense of catalytic proficiency, a trade-off of potential relevance to laccase optimization. PMID:24915287

Remotely-Sensed Geology from Lander-Based to Orbital Perspectives: Results for FIDO Rover Field Tests

NASA Technical Reports Server (NTRS)

Jolliff, B.; Moersch, J.; Knoll, A.; Morris, R.; Arvidson, R.; Gilmore, M.; Greeley, R.; Herkenhoff, K.; McSween, H.; Squyres, S.

2000-01-01

Tests of the FIDO (Field Integration Design and Operations) rover and Athena-like operational scenarios were conducted May 7-16, 2000. A group located at the Jet Propulsion Lab, Pasadena, CA, formed the Core Operations Team (COT) that designed experiments and command sequences while another team tracked, maintained, and secured the rover in the field. The COT had no knowledge of the specific field location, thus the tests were done "blind." In addition to FIDO rover instrumentation, the COT had access to LANDSAT 7, TIMS, and AVIRIS regional coverage and color descent images. Using data from the FIDO instruments, primarily a color microscopic imager (CMI), infrared point spectrometer (IPS; 1.5-2.4 microns), and a three-color stereo panoramic camera (Pancam), the COT correlated lithologic features (mineralogy, rock types) from the simulated landing site to a regional scale. The May test results provide an example of how to relate site geology from landed rover investigations to the regional geology using remote sensing. The capability to relate mineralogic signatures using the point IR spectrometer to remotely sensed, multispectral or hyperspectral data proved to be key to integration of the in-situ and remote data. This exercise demonstrated the potential synergy between lander-based and orbital data, and highlighted the need to investigate a landing site in detail and at multiple scales.

Minimum cost of transport in Asian elephants: do we really need a bigger elephant?

PubMed

Langman, Vaughan A; Rowe, Michael F; Roberts, Thomas J; Langman, Nathanial V; Taylor, Charles R

2012-05-01

Body mass is the primary determinant of an animal's energy requirements. At their optimum walking speed, large animals have lower mass-specific energy requirements for locomotion than small ones. In animals ranging in size from 0.8 g (roach) to 260 kg (zebu steer), the minimum cost of transport (COT(min)) decreases with increasing body size roughly as COT(min)∝body mass (M(b))(-0.316±0.023) (95% CI). Typically, the variation of COT(min) with body mass is weaker at the intraspecific level as a result of physiological and geometric similarity within closely related species. The interspecific relationship estimates that an adult elephant, with twice the body mass of a mid-sized elephant, should be able to move its body approximately 23% cheaper than the smaller elephant. We sought to determine whether adult Asian and sub-adult African elephants follow a single quasi-intraspecific relationship, and extend the interspecific relationship between COT(min) and body mass to 12-fold larger animals. Physiological and possibly geometric similarity between adult Asian elephants and sub-adult African elephants caused body mass to have a no effect on COT(min) (COT(min)∝M(b)(0.007±0.455)). The COT(min) in elephants occurred at walking speeds between 1.3 and ∼1.5 m s(-1), and at Froude numbers between 0.10 and 0.24. The addition of adult Asian elephants to the interspecific relationship resulted in COT(min)∝M (-0.277±0.046)(b). The quasi-intraspecific relationship between body mass and COT(min) among elephants caused the interspecific relationship to underestimate COT(min) in larger elephants.

Sink fast and swim harder! Round-trip cost-of-transport for buoyant divers.

PubMed

Miller, Patrick J O; Biuw, Martin; Watanabe, Yuuki Y; Thompson, Dave; Fedak, Mike A

2012-10-15

Efficient locomotion between prey resources at depth and oxygen at the surface is crucial for breath-hold divers to maximize time spent in the foraging layer, and thereby net energy intake rates. The body density of divers, which changes with body condition, determines the apparent weight (buoyancy) of divers, which may affect round-trip cost-of-transport (COT) between the surface and depth. We evaluated alternative predictions from external-work and actuator-disc theory of how non-neutral buoyancy affects round-trip COT to depth, and the minimum COT speed for steady-state vertical transit. Not surprisingly, the models predict that one-way COT decreases (increases) when buoyancy aids (hinders) one-way transit. At extreme deviations from neutral buoyancy, gliding at terminal velocity is the minimum COT strategy in the direction aided by buoyancy. In the transit direction hindered by buoyancy, the external-work model predicted that minimum COT speeds would not change at greater deviations from neutral buoyancy, but minimum COT speeds were predicted to increase under the actuator disc model. As previously documented for grey seals, we found that vertical transit rates of 36 elephant seals increased in both directions as body density deviated from neutral buoyancy, indicating that actuator disc theory may more closely predict the power requirements of divers affected by gravity than an external work model. For both models, minor deviations from neutral buoyancy did not affect minimum COT speed or round-trip COT itself. However, at body-density extremes, both models predict that savings in the aided direction do not fully offset the increased COT imposed by the greater thrusting required in the hindered direction.

Cargo Commercial Orbital Transportation Services Environmental Control and Life Support Integration

NASA Technical Reports Server (NTRS)

Duchesne, Stephanie; Thacker, Karen; Williams, Dave

2012-01-01

The International Space Station s (ISS) largest crew and cargo resupply vehicle, the Space Shuttle, retired in 2011. To help augment ISS resupply and return capability, NASA announced a project to promote the development of Commercial Orbital Transportation Services (COTS) for the ISS in January of 2006. By December of 2008, NASA entered into space act agreements with SpaceX and Orbital Sciences Corporation for COTS development and ISS Commercial Resupply Services (CRS). The intent of CRS is to fly multiple resupply missions each year to ISS with SpaceX s Dragon vehicle providing resupply and return capabilities and Orbital Science Corporation s Cygnus vehicle providing resupply capability to ISS. The ISS program launched an integration effort to ensure that these new commercial vehicles met the requirements of the ISS vehicle and ISS program needs. The Environmental Control and Life Support System (ECLSS) requirements cover basic cargo vehicle needs including maintaining atmosphere, providing atmosphere circulation, and fire detection and suppression. The ISS-COTS integration effort brought unique challenges combining NASA s established processes and design knowledge with the commercial companies new initiatives and limited experience with human space flight. This paper will discuss the ISS ECLS COTS integration effort including challenges, successes, and lessons learned.

Commercial Orbital Transportation Cargo Services Environmental Control and Life Support Integration

NASA Technical Reports Server (NTRS)

Duchesne, Stephanie; Williams, Dave; Orozco, Nicole; Philistine, Cynthia

2010-01-01

The International Space Station s (ISS) largest crew and cargo resupply vehicle, the Space Shuttle, will retire in 2011. To help augment ISS resupply and return capability, NASA announced a project to promote the development of Commercial Orbital Transportation Services (COTS) for the ISS in January of 2006. By December of 2008, NASA entered into space act agreements with SpaceX and Orbital Sciences Corporation for COTS development and ISS Commercial Resupply Services (CRS). The intent of CRS is to fly multiple resupply missions each year to ISS with SpaceX s Dragon vehicle providing resupply and return capabilities and Orbital Science Corporation s Cygnus vehicle providing resupply capability to ISS. The ISS program launched an integration effort to ensure that these new commercial vehicles met the requirements of the ISS vehicle and ISS program needs. The Environmental Control and Life Support System (ECLSS) requirements cover basic cargo vehicle needs including maintaining atmosphere, providing atmosphere circulation, and fire detection and suppression. The ISS-COTS integration effort brought unique challenges combining NASA s established processes and design knowledge with the commercial companies new initiatives and limited experience with human space flight. This paper will discuss the ISS ECLS COTS integration effort including challenges, successes, and lessons learned.

RASSP Final Technical Report.

DTIC Science & Technology

1992-10-21

Virtual Prototyping Support 5-27 5.3.6.3 Hardware/Software Co-design Support 5-27 5.3.6.4 Mixed Analog/Digital Design Support 5-28 5.3.6.5 VHDL Modeling...0.6 0.3 50 1 1 1 73.2 23.4 19.6" 84 "kIuded $11.M p•rmhed undwe contr" "kh"ded SUM pcsed undr c SDollm Include purchae of poducts , malntenmce, ard...year on utilization of standards and COTS technology however does result in an achievable system performance which is less that what would be

Platelet-derived growth factor regulates K-Cl cotransport in vascular smooth muscle cells.

PubMed

Zhang, Jing; Lauf, Peter K; Adragna, Norma C

2003-03-01

Platelet-derived growth factor (PDGF), a potent serum mitogen for vascular smooth muscle cells (VSMCs), plays an important role in membrane transport regulation and in atherosclerosis. K-Cl cotransport (K-Cl COT/KCC), the coupled-movement of K and Cl, is involved in ion homeostasis. VSMCs possess K-Cl COT activity and the KCC1 and KCC3 isoforms. Here, we report on the effect of PDGF on K-Cl COT activity and mRNA expression in primary cultures of rat VSMCs. K-Cl COT was determined as the Cl-dependent Rb influx and mRNA expression by semiquantitative RT-PCR. Twenty four-hour serum deprivation inhibited basal K-Cl COT activity. Addition of PDGF increased total protein content and K-Cl COT activity in a time-dependent manner. PDGF activated K-Cl COT in a dose-dependent manner, both acutely (10 min) and chronically (12 h). AG-1296, a selective inhibitor of the PDGF receptor tyrosine kinase, abolished these effects. Actinomycin D and cycloheximide had no effect on the acute PDGF activation of K-Cl COT, suggesting posttranslational regulation by the drug. Furthermore, PDGF increased KCC1 and decreased KCC3 mRNA expression in a time-dependent manner. These results indicate that chronic activation of K-Cl COT activity by PDGF may involve regulation of the two KCC mRNA isoforms, with KCC1 playing a dominant role in the mechanism of PDGF-mediated activation.

A serine/threonine kinase, Cot/Tpl2, modulates bacterial DNA-induced IL-12 production and Th cell differentiation.

PubMed

Sugimoto, Kenji; Ohata, Mutsuhiro; Miyoshi, Jun; Ishizaki, Hiroyoshi; Tsuboi, Naotake; Masuda, Akio; Yoshikai, Yasunobu; Takamoto, Masaya; Sugane, Kazuo; Matsuo, Seiichi; Shimada, Yasuhiro; Matsuguchi, Tetsuya

2004-09-01

A serine/threonine protein kinase, Cot/Tpl2, is indispensable for extracellular signal-regulated kinase (ERK) activation and production of TNF-alpha and PGE2 in LPS-stimulated macrophages. We show here that Cot/Tpl2 is also activated by other Toll-like receptor (TLR) ligands. Bacterial DNA rich in the dinucleotide CG (CpG-DNA), unlike LPS or synthetic lipopeptide, activated ERK in a Cot/Tpl2-independent manner. Peritoneal macrophages and bone marrow-derived DCs from Cot/Tpl2-/- mice produced significantly more IL-12 in response to CpG-DNA than those from WT mice. Enhanced IL-12 production in Cot/Tpl2-/- macrophages is, at least partly, regulated at the transcriptional level, and the elevated IL-12 mRNA level in Cot/Tpl2-/- macrophages is accompanied by decreased amounts of IL-12 repressors, such as c-musculoaponeurotic fibrosarcoma (c-Maf) and GATA sequence in the IL-12 promoter-binding protein (GA-12-binding protein; GAP-12) in the nucleus. Consistently, Cot/Tpl2-/- mice showed Th1-skewed antigen-specific immune responses upon OVA immunization and Leishmania major infection in vivo. These results indicate that Cot/Tpl2 is an important negative regulator of Th1-type adaptive immunity, that it achieves this regulation by inhibiting IL-12 production from accessory cells, and that it might be a potential target molecule in CpG-DNA-guided vaccination.

CRAX/Cassandra Reliability Analysis Software

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robinson, D.

1999-02-10

Over the past few years Sandia National Laboratories has been moving toward an increased dependence on model- or physics-based analyses as a means to assess the impact of long-term storage on the nuclear weapons stockpile. These deterministic models have also been used to evaluate replacements for aging systems, often involving commercial off-the-shelf components (COTS). In addition, the models have been used to assess the performance of replacement components manufactured via unique, small-lot production runs. In either case, the limited amount of available test data dictates that the only logical course of action to characterize the reliability of these components ismore » to specifically consider the uncertainties in material properties, operating environment etc. within the physics-based (deterministic) model. This not only provides the ability to statistically characterize the expected performance of the component or system, but also provides direction regarding the benefits of additional testing on specific components within the system. An effort was therefore initiated to evaluate the capabilities of existing probabilistic methods and, if required, to develop new analysis methods to support the inclusion of uncertainty in the classical design tools used by analysts and design engineers at Sandia. The primary result of this effort is the CMX (Cassandra Exoskeleton) reliability analysis software.« less

Data Relay Board with Protocol for High-Speed, Free-Space Optical Communications

NASA Technical Reports Server (NTRS)

Wright, Malcolm; Clare, Loren; Gould, Gary; Pedyash, Maxim

2004-01-01

In a free-space optical communication system, the mitigation of transient outages through the incorporation of error-control methods is of particular concern, the outages being caused by scintillation fades and obscurants. The focus of this innovative technology is the development of a data relay system for a reliable high-data-rate free-spacebased optical-transport network. The data relay boards will establish the link, maintain synchronous connection, group the data into frames, and provide for automatic retransmission (ARQ) of lost or erred frames. A certain Quality of Service (QoS) can then be ensured, compatible with the required data rate. The protocol to be used by the data relay system is based on the draft CCSDS standard data-link protocol Proximity-1, selected by orbiters to multiple lander assets in the Mars network, for example. In addition to providing data-link protocol capabilities for the free-space optical link and buffering the data, the data relay system will interface directly with user applications over Gigabit Ethernet and/or with highspeed storage resources via Fibre Channel. The hardware implementation is built on a network-processor-based architecture. This technology combines the power of a hardware switch capable of data switching and packet routing at Gbps rates, with the flexibility of a software- driven processor that can host highly adaptive and reconfigurable protocols used, for example, in wireless local-area networks (LANs). The system will be implemented in a modular multi-board fashion. The main hardware elements of the data relay system are the new data relay board developed by Rockwell Scientific, a COTS Gigabit Ethernet board for user interface, and a COTS Fibre Channel board that connects to local storage. The boards reside in a cPCI back plane, and can be housed in a VME-type enclosure.

Structural and magnetic conformation of a cerocene [Ce(COT'')2]- exhibiting a uniconfigurational f1 ground state and slow-magnetic relaxation.

PubMed

Le Roy, Jennifer J; Korobkov, Ilia; Kim, Jee Eon; Schelter, Eric J; Murugesu, Muralee

2014-02-21

Magnet-like behaviour, in the form of slow relaxation of the magnetization, was observed for a monometallic cerium(III) sandwich complex. The use of trimethylsilyl substituted COT ligands (COT'') led to the formation of a staggered COT'' arrangement in the cerocene-type sandwich complex with a well-defined oxidation state of +3 for the Ce ion.

COT phosphorylates prolyl-isomerase Pin1 to promote tumorigenesis in breast cancer.

PubMed

Kim, Garam; Khanal, Prem; Kim, Jin Young; Yun, Hyo-Jeong; Lim, Sung-Chul; Shim, Jung-Hyun; Choi, Hong Seok

2015-06-01

Pin1, a conserved eukaryotic Peptidyl-prolyl cis/trans isomerase, has profound effects on numerous key-signaling molecules, and its deregulation contributes to disease, particularly cancer. Although Pin1-mediated prolyl isomerization is an essential and novel regulatory mechanism for protein phosphorylation, little is known about the upstream signaling pathway(s) that regulates Pin1 activity. Here, we identify MAP3K-related serine-threonine kinase (the gene encoding COT/Tpl2) as a kinase responsible for phosphorylation of Pin1 Ser16. COT interacts with and phosphorylates Pin1 on Ser16. Consequently, Pin1 Ser16 phosphorylation by COT increases cyclin D1 abundance and enhances tumorigenecity of MCF7 cells. In contrast, depletion of COT in MCF7 cells leads to downregulation of Pin1 Ser16 phosphorylation, which subsequently decrease cyclin D1 levels, inhibiting tumorigenecity of MCF7 cells. In a xenograft model, treatment of TKI, a COT inhibitor, and Juglone, a Pin1 inhibitor, abrogates tumor growth. In human breast cancer patients, immunohistochemical staining shows that Pin1 pSer16 levels are positively correlated with COT levels, providing strong evidence for an essential role of the COT/Pin1 axis in conveying oncogenic signals to promote aggressiveness in human breast cancer. © 2013 Wiley Periodicals, Inc.

Benchmarking desktop and mobile handwriting across COTS devices: The e-BioSign biometric database

PubMed Central

Tolosana, Ruben; Vera-Rodriguez, Ruben; Fierrez, Julian; Morales, Aythami; Ortega-Garcia, Javier

2017-01-01

This paper describes the design, acquisition process and baseline evaluation of the new e-BioSign database, which includes dynamic signature and handwriting information. Data is acquired from 5 different COTS devices: three Wacom devices (STU-500, STU-530 and DTU-1031) specifically designed to capture dynamic signatures and handwriting, and two general purpose tablets (Samsung Galaxy Note 10.1 and Samsung ATIV 7). For the two Samsung tablets, data is collected using both pen stylus and also the finger in order to study the performance of signature verification in a mobile scenario. Data was collected in two sessions for 65 subjects, and includes dynamic information of the signature, the full name and alpha numeric sequences. Skilled forgeries were also performed for signatures and full names. We also report a benchmark evaluation based on e-BioSign for person verification under three different real scenarios: 1) intra-device, 2) inter-device, and 3) mixed writing-tool. We have experimented the proposed benchmark using the main existing approaches for signature verification: feature- and time functions-based. As a result, new insights into the problem of signature biometrics in sensor-interoperable scenarios have been obtained, namely: the importance of specific methods for dealing with device interoperability, and the necessity of a deeper analysis on signatures acquired using the finger as the writing tool. This e-BioSign public database allows the research community to: 1) further analyse and develop signature verification systems in realistic scenarios, and 2) investigate towards a better understanding of the nature of the human handwriting when captured using electronic COTS devices in realistic conditions. PMID:28475590

Benchmarking desktop and mobile handwriting across COTS devices: The e-BioSign biometric database.

PubMed

Tolosana, Ruben; Vera-Rodriguez, Ruben; Fierrez, Julian; Morales, Aythami; Ortega-Garcia, Javier

2017-01-01

This paper describes the design, acquisition process and baseline evaluation of the new e-BioSign database, which includes dynamic signature and handwriting information. Data is acquired from 5 different COTS devices: three Wacom devices (STU-500, STU-530 and DTU-1031) specifically designed to capture dynamic signatures and handwriting, and two general purpose tablets (Samsung Galaxy Note 10.1 and Samsung ATIV 7). For the two Samsung tablets, data is collected using both pen stylus and also the finger in order to study the performance of signature verification in a mobile scenario. Data was collected in two sessions for 65 subjects, and includes dynamic information of the signature, the full name and alpha numeric sequences. Skilled forgeries were also performed for signatures and full names. We also report a benchmark evaluation based on e-BioSign for person verification under three different real scenarios: 1) intra-device, 2) inter-device, and 3) mixed writing-tool. We have experimented the proposed benchmark using the main existing approaches for signature verification: feature- and time functions-based. As a result, new insights into the problem of signature biometrics in sensor-interoperable scenarios have been obtained, namely: the importance of specific methods for dealing with device interoperability, and the necessity of a deeper analysis on signatures acquired using the finger as the writing tool. This e-BioSign public database allows the research community to: 1) further analyse and develop signature verification systems in realistic scenarios, and 2) investigate towards a better understanding of the nature of the human handwriting when captured using electronic COTS devices in realistic conditions.

Validation of software for calculating the likelihood ratio for parentage and kinship.

PubMed

Drábek, J

2009-03-01

Although the likelihood ratio is a well-known statistical technique, commercial off-the-shelf (COTS) software products for its calculation are not sufficiently validated to suit general requirements for the competence of testing and calibration laboratories (EN/ISO/IEC 17025:2005 norm) per se. The software in question can be considered critical as it directly weighs the forensic evidence allowing judges to decide on guilt or innocence or to identify person or kin (i.e.: in mass fatalities). For these reasons, accredited laboratories shall validate likelihood ratio software in accordance with the above norm. To validate software for calculating the likelihood ratio in parentage/kinship scenarios I assessed available vendors, chose two programs (Paternity Index and familias) for testing, and finally validated them using tests derived from elaboration of the available guidelines for the field of forensics, biomedicine, and software engineering. MS Excel calculation using known likelihood ratio formulas or peer-reviewed results of difficult paternity cases were used as a reference. Using seven testing cases, it was found that both programs satisfied the requirements for basic paternity cases. However, only a combination of two software programs fulfills the criteria needed for our purpose in the whole spectrum of functions under validation with the exceptions of providing algebraic formulas in cases of mutation and/or silent allele.

Multi-species consumer jams and the fall of guarded corals to crown-of-thorns seastar outbreaks

PubMed Central

Kayal, Mohsen; Ballard, Jane; Adjeroud, Mehdi

2018-01-01

Outbreaks of predatory crown-of-thorns seastars (COTS) can devastate coral reef ecosystems, yet some corals possess mutualistic guardian crabs that defend against COTS attacks. However, guarded corals do not always survive COTS outbreaks, with the ecological mechanisms sealing the fate of these corals during COTS infestations remaining unknown. In August 2008 in Moorea (17.539° S, 149.830° W), French Polynesia, an unusually dense multi-species aggregation of predators was observed feeding upon guarded corals following widespread coral decline due to COTS predation. Concurrent assaults from these amplified, mixed-species predator guilds likely overwhelm mutualistic crab defense, ultimately leading to the fall of guarded corals. Our observations indicate that guarded corals can sustain devastating COTS attacks for an extended duration, but eventually concede to intensifying assaults from diverse predators that aggregate in high numbers as alternative prey decays. The fall of guarded corals is therefore suggested to be ultimately driven by an indirect trophic cascade that leads to amplified attacks from diverse starving predators following prey decline, rather than COTS assaults alone. PMID:29487739

PDGF activates K-Cl cotransport through phosphoinositide 3-kinase and protein phosphatase-1 in primary cultures of vascular smooth muscle cells.

PubMed

Zhang, Jing; Lauf, Peter K; Adragna, Norma C

2005-07-15

K-Cl cotransport (K-Cl COT, KCC) is an electroneutrally coupled movement of K and Cl present in most cells. In this work, we studied the pathways of regulation of K-Cl COT by platelet-derived growth factor (PDGF) in primary cultures of vascular smooth muscle cells (VSMCs). Wortmannin and LY 294002 blocked the PDGF-induced K-Cl COT activation, indicating that the phosphoinositide 3-kinase (PI 3-K) pathway is involved. However, PD 98059 had no effect on K-Cl COT activation by PDGF, suggesting that the mitogen-activated protein kinase pathway is not involved under the experimental conditions tested. Involvement of phosphatases was also examined. Sodium orthovanadate, cyclosporin A and okadaic acid had no effect on PDGF-stimulated K-Cl COT. Calyculin A blocked the PDGF-stimulated K-Cl COT by 60%, suggesting that protein phosphatase-1 (PP-1) is a mediator in the PDGF signaling pathway/s. In conclusion, our results indicate that the PDGF-mediated pathways of K-Cl COT regulation involve the signaling molecules PI 3-K and PP-1.

Involvement of Cot activity in the proliferation of ALCL lymphoma cells.

PubMed

Fernández, Margarita; Manso, Rebeca; Bernaldo de Quirós, Flavia; Bernáldez, Flavia; López, Pilar; Martín-Duce, Antonio; Alemany, Susana

2011-08-12

Anaplastic large-cell lymphoma (ALCL) cells overexpress CD30 on their cell surface, show increased levels of activated Erk1/2 and of JunB; participating JunB in the proliferative capacity of these lymphomas. Here, we show that ALCL lymphoma cells also present high expression levels of the proto-oncogenic Cot (MAP3K8). Using pharmacological drugs as well as the RNA interference technique we show that Cot protein is responsible for the constitutive Erk1/2 activation in the ALCL lymphoma cells, SUDHL-1. Besides, inhibition of Cot activity reduces the number of cell divisions which is achieved, at least in part, by the control that Cot exercises on the activation state of p70 S6K and on the expression levels of JunB. Since Cot represents an alternative mode, independently of RAF, to activate Erk1/2, all these data strongly suggest that molecular targeting of Cot may be a potential new specific strategy for ALCL lymphomas therapy, without the fully disturbance of the Erk1/2 function. Copyright © 2011. Published by Elsevier Inc.

NASA and COTS Electronics: Past Approach and Successes - Future Considerations

NASA Technical Reports Server (NTRS)

LaBel, Kenneth A.

2018-01-01

NASA has a long history of using commercial grade electronics in space. In this talk, a brief history of NASAâ's trends and approaches to commercial grade electronics focusing on processing and memory systems will be presented. This will include providing summary information on the space hazards to electronics as well as NASA mission trade space. We will also discuss developing recommendations for risk management approaches to Electrical, Electronic and Electromechanical (EEE) parts and reliability in space. The final portion of the talk will discuss emerging aerospace trends and the future for Commercial Off The Shelf (COTS) usage.

Estimation of cloud optical thickness by processing SEVIRI images and implementing a semi analytical cloud property retrieval algorithm

NASA Astrophysics Data System (ADS)

Pandey, P.; De Ridder, K.; van Lipzig, N.

2009-04-01

Clouds play a very important role in the Earth's climate system, as they form an intermediate layer between Sun and the Earth. Satellite remote sensing systems are the only means to provide information about clouds on large scales. The geostationary satellite, Meteosat Second Generation (MSG) has onboard an imaging radiometer, the Spinning Enhanced Visible and Infrared Imager (SEVIRI). SEVIRI is a 12 channel imager, with 11 channels observing the earth's full disk with a temporal resolution of 15 min and spatial resolution of 3 km at nadir, and a high resolution visible (HRV) channel. The visible channels (0.6 µm and 0.81 µm) and near infrared channel (1.6µm) of SEVIRI are being used to retrieve the cloud optical thickness (COT). The study domain is over Europe covering the region between 35°N - 70°N and 10°W - 30°E. SEVIRI level 1.5 images over this domain are being acquired from the European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT) archive. The processing of this imagery, involves a number of steps before estimating the COT. The steps involved in pre-processing are as follows. First, the digital count number is acquired from the imagery. Image geo-coding is performed in order to relate the pixel positions to the corresponding longitude and latitude. Solar zenith angle is determined as a function of latitude and time. The radiometric conversion is done using the values of offsets and slopes of each band. The values of radiance obtained are then used to calculate the reflectance for channels in the visible spectrum using the information of solar zenith angle. An attempt is made to estimate the COT from the observed radiances. A semi analytical algorithm [Kokhanovsky et al., 2003] is implemented for the estimation of cloud optical thickness from the visible spectrum of light intensity reflected from clouds. The asymptotical solution of the radiative transfer equation, for clouds with large optical thickness, is the basis of this algorithm. The two visible channels of SEVIRI are used to find the COT and the near infra red channel to estimate the effective radius of droplets. Estimation of COT using a semi analytical scheme, which doesn't involve the conventional look-up table approach, is the aim of this work and henceforth, vertically integrated liquid water (w) or ice water content will be retrieved. The COT estimated and w obtained, will be compared with the values obtained from other approaches and will be validated with in situ measurements. Corresponding author address: Praveen Pandey, VITO - Flemish Institute for Technological Research, Boeretang 200, B 2400, Mol, Belgium. E-mail: praveen.pandey@vito.be

Microphysical properties and ice particle morphology of cirrus clouds inferred from combined CALIOP-IIR measurements

NASA Astrophysics Data System (ADS)

Saito, M.; Iwabuchi, H.; Yang, P.; Tang, G.; King, M. D.; Sekiguchi, M.

2016-12-01

Cirrus clouds cover about 25% of the globe. Knowledge about the optical and microphysical properties of these clouds [particularly, optical thickness (COT) and effective radius (CER)] is essential to radiative forcing assessment. Previous studies of those properties using satellite remote sensing techniques based on observations by passive and active sensors gave inconsistent retrievals. In particular, COTs from the Cloud Aerosol Lidar with Orthogonal Polarization (CALIOP) using the unconstrained method are affected by variable particle morphology, especially the fraction of horizontally oriented plate particles (HPLT), because the method assumes the lidar ratio to be constant, which should have different values for different ice particle shapes. More realistic ice particle morphology improves estimates of the optical and microphysical properties. In this study, we develop an optimal estimation-based algorithm to infer cirrus COT and CER in addition to morphological parameters (e.g., Fraction of HPLT) using the observations made by CALIOP and the Infrared Imaging Radiometer (IIR) on the CALIPSO platform. The assumed ice particle model is a mixture of a few habits with variable HPLT. Ice particle single-scattering properties are computed using state-of-the-art light-scattering computational capabilities. Rigorous estimation of uncertainties associated with surface properties, atmospheric gases and cloud heterogeneity is performed. The results based on the present method show that COTs are quite consistent with the MODIS and CALIOP counterparts, and CERs essentially agree with the IIR operational retrievals. The lidar ratio is calculated from the bulk optical properties based on the inferred parameters. The presentation will focus on latitudinal variations of particle morphology and the lidar ratio on a global scale.

Technology insertion of a COTS RAID server as an image buffer in the image chain of the Defense Mapping Agency's Digital Production System

NASA Astrophysics Data System (ADS)

Mehring, James W.; Thomas, Scott D.

1995-11-01

The Data Services Segment of the Defense Mapping Agency's Digital Production System provides a digital archive of imagery source data for use by DMA's cartographic user's. This system was developed in the mid-1980's and is currently undergoing modernization. This paper addresses the modernization of the imagery buffer function that was performed by custom hardware in the baseline system and is being replaced by a RAID Server based on commercial off the shelf (COTS) hardware. The paper briefly describes the baseline DMA image system and the modernization program, that is currently under way. Throughput benchmark measurements were made to make design configuration decisions for a commercial off the shelf (COTS) RAID Server to perform as system image buffer. The test program began with performance measurements of the RAID read and write operations between the RAID arrays and the server CPU for RAID levels 0, 5 and 0+1. Interface throughput measurements were made for the HiPPI interface between the RAID Server and the image archive and processing system as well as the client side interface between a custom interface board that provides the interface between the internal bus of the RAID Server and the Input- Output Processor (IOP) external wideband network currently in place in the DMA system to service client workstations. End to end measurements were taken from the HiPPI interface through the RAID write and read operations to the IOP output interface.

Volume serving and media management in a networked, distributed client/server environment

NASA Technical Reports Server (NTRS)

Herring, Ralph H.; Tefend, Linda L.

1993-01-01

The E-Systems Modular Automated Storage System (EMASS) is a family of hierarchical mass storage systems providing complete storage/'file space' management. The EMASS volume server provides the flexibility to work with different clients (file servers), different platforms, and different archives with a 'mix and match' capability. The EMASS design considers all file management programs as clients of the volume server system. System storage capacities are tailored to customer needs ranging from small data centers to large central libraries serving multiple users simultaneously. All EMASS hardware is commercial off the shelf (COTS), selected to provide the performance and reliability needed in current and future mass storage solutions. All interfaces use standard commercial protocols and networks suitable to service multiple hosts. EMASS is designed to efficiently store and retrieve in excess of 10,000 terabytes of data. Current clients include CRAY's YMP Model E based Data Migration Facility (DMF), IBM's RS/6000 based Unitree, and CONVEX based EMASS File Server software. The VolSer software provides the capability to accept client or graphical user interface (GUI) commands from the operator's console and translate them to the commands needed to control any configured archive. The VolSer system offers advanced features to enhance media handling and particularly media mounting such as: automated media migration, preferred media placement, drive load leveling, registered MediaClass groupings, and drive pooling.

Dosimetry and microdosimetry using COTS ICs: A comparative study

NASA Technical Reports Server (NTRS)

Scheick, L.; Swift, G.; Guertin, S.; Roth, D.; McNulty, P.; Nguyen, D.

2002-01-01

A new method using an array of MOS transistors formeasuring dose absorbed from ionizing radiation is compared to previous dosimetric methods., The accuracy and precision of dosimetry based on COTS SRAMs, DRAMs, and WPROMs are compared and contrasted. Applications of these devices in various space missions will be discussed. TID results are presented for this summary and microdosimetricresults will be added to the full paper. Finally, an analysis of the optimal condition for a digital dosimeter will be presented.

How does cot death prevention advice influence parents' behaviour?

PubMed

Miller, L H; Fraser, A; Moy, R

2008-09-01

To explore parents' understanding of the recommended cot death prevention strategies, and to discuss what they are doing in practice. If there is a difference between knowledge and implementation measures, possible reasons for this will be considered. A qualitative study using thematic analysis, aimed at finding out attitudes and opinions of parents about cot death prevention measures. Twelve participants were interviewed from two disadvantaged communities in south Birmingham. Parents found that much of the cot death prevention advice they were provided with was conflicting and caused confusion. As such, many parents chose to follow advice from non-healthcare sources. Some parents were carrying out preventative measures but were not aware of the reason for doing it. Many felt they did not receive enough advice relating to cot death prevention. Cot death health promotion advice appears to be inadequate among patients from a deprived socioeconomic background. Some of these issues could be resolved with increased training addressing these matters.

Credibility gap? Parents' beliefs about reducing the risk of cot death.

PubMed

Epstein, Joyce; Jolly, Clare

2009-11-01

This paper reports on findings from a survey of awareness and credibility of risk factors for sudden infant death and adherence to infant care advice, conducted with 506 mothers of children aged from six months to three years in England in 2007. The survey confirmed that many parents failed to follow recommended infant care practices to reduce the risk of cot death, such as supine sleeping and sleeping the baby in a cot in the same room as them. Notably, substantial proportions of mothers did not believe the evidence-based safe infant care messages that they have heard--about a fifth were not persuaded that exposing a baby to tobacco smoke increases the risk of cot death, over a quarter were not persuaded that prone sleeping is risky, and almost half were not persuaded that side sleeping is risky. The findings are discussed in the context of wider cultural issues bearing on the credibility of health advice. Practical suggestions for health visitors and other community practitioners include innovative ways to involve young, at-risk parents and credible influences upon them such as grandparents, alongside the use and promotion of new free resources from the Foundation for the Study of Infant Deaths.

Branching and competition of ultrafast photochemical reactions of cyclooctatriene and bicyclooctadiene

NASA Astrophysics Data System (ADS)

Kosma, Kyriaki; Trushin, Sergei A.; Schmid, Wolfram E.; Fuß, Werner

2015-12-01

The main primary photoproducts of cycloocta-1,3,5-triene (COT) are a strained mono-E isomer, Z,Z-octatetraene (OT, from electrocyclic ring opening) and benzene + ethylene. We investigated the excited-state dynamics of COT by time-resolved mass spectroscopy, probing by near-IR photoionization. Unexpectedly, we found only one reaction channel. We assign it to the pericyclic reactions. Evidence for an early branching between this and the Z-E channel is taken from previous resonance Raman data. This channel confirms previously formulated rules on the excited states involved, the reaction path and driving forces and contributes to their rationalization. Bicyclo[4.2.0]octa-2,4-diene undergoes only two pericyclic reactions: ring opening to OT and cleavage to benzene + ethylene. We investigated it briefly in its equilibrium mixture with COT. The data are consistent with a common path on the excited surfaces. Suggestions are made for structures of conical intersections, and driving forces are considered. All processes were found to be barrierless.

Regulation of K-Cl cotransport: from function to genes.

PubMed

Adragna, N C; Di Fulvio, M; Lauf, P K

2004-10-01

This review intends to summarize the vast literature on K-Cl cotransport (COT) regulation from a functional and genetic viewpoint. Special attention has been given to the signaling pathways involved in the transporter's regulation found in several tissues and cell types, and more specifically, in vascular smooth muscle cells (VSMCs). The number of publications on K-Cl COT has been steadily increasing since its discovery at the beginning of the 1980s, with red blood cells (RBCs) from different species (human, sheep, dog, rabbit, guinea pig, turkey, duck, frog, rat, mouse, fish, and lamprey) being the most studied model. Other tissues/cell types under study are brain, kidney, epithelia, muscle/smooth muscle, tumor cells, heart, liver, insect cells, endothelial cells, bone, platelets, thymocytes and Leishmania donovani. One of the salient properties of K-Cl-COT is its activation by cell swelling and its participation in the recovery of cell volume, a process known as regulatory volume decrease (RVD). Activation by thiol modification with N-ethylmaleimide (NEM) has spawned investigations on the redox dependence of K-Cl COT, and is used as a positive control for the operation of the system in many tissues and cells. The most accepted model of K-Cl COT regulation proposes protein kinases and phosphatases linked in a chain of phosphorylation/dephosphorylation events. More recent studies include regulatory pathways involving the phosphatidyl inositol/protein kinase C (PKC)-mediated pathway for regulation by lithium (Li) in low-K sheep red blood cells (LK SRBCs), and the nitric oxide (NO)/cGMP/protein kinase G (PKG) pathway as well as the platelet-derived growth factor (PDGF)-mediated mechanism in VSMCs. Studies on VSM transfected cells containing the PKG catalytic domain demonstrated the participation of this enzyme in K-Cl COT regulation. Commonly used vasodilators activate K-Cl COT in a dose-dependent manner through the NO/cGMP/PKG pathway. Interaction between the cotransporter and the cytoskeleton appears to depend on the cellular origin and experimental conditions. Pathophysiologically, K-Cl COT is altered in sickle cell anemia and neuropathies, and it has also been proposed to play a role in blood pressure control. Four closely related human genes code for KCCs (KCC1-4). Although considerable information is accumulating on tissue distribution, function and pathologies associated with the different isoforms, little is known about the genetic regulation of the KCC genes in terms of transcriptional and post-transcriptional regulation. A few reports indicate that the NO/cGMP/PKG signaling pathway regulates KCC1 and KCC3 mRNA expression in VSMCs at the post-transcriptional level. However, the detailed mechanisms of post-transcriptional regulation of KCC genes and of regulation of KCC2 and KCC4 mRNA expression are unknown. The K-Cl COT field is expected to expand further over the next decades, as new isoforms and/or regulatory pathways are discovered and its implication in health and disease is revealed.

Effect on Oxygen Cost of Transport from 8-Weeks of Progressive Training with Barefoot Running.

PubMed

Tam, N; Tucker, R; Astephen Wilson, J L; Santos-Concejero, J

2015-11-01

Popular interest in barefoot running has emerged as a result of its alleged performance and injury prevention benefits. Oxygen cost of transport (COT) improvements from barefoot running, however, remains equivocal. The aim of this study was to determine the influence of an 8-week progressive barefoot training program on COT and associated spatiotemporal variables. 15 male runners participated in this study. Variables such as oxygen uptake, biomechanical and spatiotemporal characteristics of gait, including ground contact (GC) and swing time; stride length and frequency and ankle plantar-dorsiflexion were measured pre- and post-intervention. The COT did not differ between barefoot and shod running either pre- or post-training. Improved barefoot COT (p<0.05) but not shod was found between pre- and post-training. Biomechanical differences between barefoot and shod conditions persisted over the training period. A decrease in barefoot COT was associated with a decrease in GC time (p=0.003, r=0.688) and a small increase in stride frequency (p=0.030; r=0.569). Ground contact time and stride frequency, previously associated with COT, only partly contribute (32% - Stride frequency and 47% - GC time) to a decrease in COT after barefoot training. Thus other physiological and biomechanical variables must influence the improvement in COT after a barefoot training intervention. © Georg Thieme Verlag KG Stuttgart · New York.

Effects of Cot expression on the nuclear translocation of NF-kappaB in RBL-2H3 cells.

PubMed

Chikamatsu, Satomi; Furuno, Tadahide; Kinoshita, Yosuke; Inoh, Yoshikazu; Hirashima, Naohide; Teshima, Reiko; Nakanishi, Mamoru

2007-03-01

Cot is a serine/threonine protein kinase and is classified as a mitogen-activated protein (MAP) kinase kinase kinase. Overexpression of this protein has been shown to activate the extracellular signal-regulated kinase, the c-Jun N-terminal kinase, and the p38 MAP kinase pathways and to stimulate NF-AT and NF-kappaB-dependent transcription. Here we have shown that Cot kinase activity is intimately involved in the high affinity receptor for IgE (FcvarepsilonRI)-mediated nuclear translocation of NF-kappaB1 independent of NF-kappaB-inducing kinase (NIK) in rat basophilic leukemia (RBL-2H3) cells. A transfected green fluorescent protein-tagged NF-kappaB1 (GFP-NF-kappaB1) resided in the cytoplasm in RBL-2H3 cells and it remained in the cytoplasm even when Cot tagged with red fluorescent protein (Cot-RFP) was co-expressed. Western blotting analysis showed that IkappaB kinases (IKKs) were expressed in RBL-2H3 cells but NIK was not. GFP-NF-kappaB1 translocated from the cytoplasm to the nucleus after the aggregation of FcvarepsilonRI in Cot-transfected cells but not in kinase-deficient Cot-transfected cells. This finding gives a new insight into the role of Cot in the FcvarepsilonRI-mediated NF-kappaB activation in mast cells.

The Neurospora crassa PP2A Regulatory Subunits RGB1 and B56 Are Required for Proper Growth and Development and Interact with the NDR Kinase COT1

PubMed Central

Shomin-Levi, Hila; Yarden, Oded

2017-01-01

COT1 is the founding member of the highly conserved nuclear Dbf2-related (NDR) Ser/Thr kinase family and plays a role in the regulation of polar growth and development in Neurospora crassa and other fungi. Changes in COT1 phosphorylation state have been shown to affect hyphal elongation, branching, and conidiation. The function of NDR protein kinases has been shown to be regulated by type 2A protein phosphatases (PP2As). PP2As are heterotrimers comprised of a catalytic and scaffolding protein along with an interchangeable regulatory subunit involved in determining substrate specificity. Inactivation of the N. crassa PP2A regulatory subunits rgb-1 and b56 conferred severe hyphal growth defects. Partial suppression of defects observed in the rgb-1RIP strain (but not in the Δb56 mutant) was observed in cot-1 phosphomimetic mutants, demonstrating that altering COT1 phosphorylation state can bypass, at least in part, the requirement of a functional RGB1 subunit. The functional fusion proteins RGB1::GFP and B56::GFP predominantly localized to hyphal tips and septa, respectively, indicating that their primary activity is in different cellular locations. COT1 protein forms exhibited a hyperphosphorylated gel migration pattern in an rgb-1RIP mutant background, similar to that observed when the fungus was cultured in the presence of the PP2A inhibitor cantharidin. COT1 was hypophosphorylated in a Δb56 mutant background, suggesting that this regulatory subunit may be involved in determining COT1 phosphorylation state, yet in an indirect manner. Reciprocal co-immunoprecipitation analyses, using tagged COT1, PPH1, RGB1, and B56 subunits established that these proteins physically interact. Taken together, our data determine the presence of a functional and physical link between PP2A and COT1 and show that two of the PP2A regulatory subunits interact with the kinase and determine COT1 phosphorylation state. PMID:28928725

Secure Web-based Ground System User Interfaces over the Open Internet

NASA Technical Reports Server (NTRS)

Langston, James H.; Murray, Henry L.; Hunt, Gary R.

1998-01-01

A prototype has been developed which makes use of commercially available products in conjunction with the Java programming language to provide a secure user interface for command and control over the open Internet. This paper reports successful demonstration of: (1) Security over the Internet, including encryption and certification; (2) Integration of Java applets with a COTS command and control product; (3) Remote spacecraft commanding using the Internet. The Java-based Spacecraft Web Interface to Telemetry and Command Handling (Jswitch) ground system prototype provides these capabilities. This activity demonstrates the use and integration of current technologies to enable a spacecraft engineer or flight operator to monitor and control a spacecraft from a user interface communicating over the open Internet using standard World Wide Web (WWW) protocols and commercial off-the-shelf (COTS) products. The core command and control functions are provided by the COTS Epoch 2000 product. The standard WWW tools and browsers are used in conjunction with the Java programming technology. Security is provided with the current encryption and certification technology. This system prototype is a step in the direction of giving scientist and flight operators Web-based access to instrument, payload, and spacecraft data.

Extended Testability Analysis Tool

NASA Technical Reports Server (NTRS)

Melcher, Kevin; Maul, William A.; Fulton, Christopher

2012-01-01

The Extended Testability Analysis (ETA) Tool is a software application that supports fault management (FM) by performing testability analyses on the fault propagation model of a given system. Fault management includes the prevention of faults through robust design margins and quality assurance methods, or the mitigation of system failures. Fault management requires an understanding of the system design and operation, potential failure mechanisms within the system, and the propagation of those potential failures through the system. The purpose of the ETA Tool software is to process the testability analysis results from a commercial software program called TEAMS Designer in order to provide a detailed set of diagnostic assessment reports. The ETA Tool is a command-line process with several user-selectable report output options. The ETA Tool also extends the COTS testability analysis and enables variation studies with sensor sensitivity impacts on system diagnostics and component isolation using a single testability output. The ETA Tool can also provide extended analyses from a single set of testability output files. The following analysis reports are available to the user: (1) the Detectability Report provides a breakdown of how each tested failure mode was detected, (2) the Test Utilization Report identifies all the failure modes that each test detects, (3) the Failure Mode Isolation Report demonstrates the system s ability to discriminate between failure modes, (4) the Component Isolation Report demonstrates the system s ability to discriminate between failure modes relative to the components containing the failure modes, (5) the Sensor Sensor Sensitivity Analysis Report shows the diagnostic impact due to loss of sensor information, and (6) the Effect Mapping Report identifies failure modes that result in specified system-level effects.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Williams, Edward J., Jr.; Henry, Karen Lynne

Sandia National Laboratories develops technologies to: (1) sustain, modernize, and protect our nuclear arsenal (2) Prevent the spread of weapons of mass destruction; (3) Provide new capabilities to our armed forces; (4) Protect our national infrastructure; (5) Ensure the stability of our nation's energy and water supplies; and (6) Defend our nation against terrorist threats. We identified the need for a single overarching Integrated Workplace Management System (IWMS) that would enable us to focus on customer missions and improve FMOC processes. Our team selected highly configurable commercial-off-the-shelf (COTS) software with out-of-the-box workflow processes that integrate strategic planning, project management, facilitymore » assessments, and space management, and can interface with existing systems, such as Oracle, PeopleSoft, Maximo, Bentley, and FileNet. We selected the Integrated Workplace Management System (IWMS) from Tririga, Inc. Facility Management System (FMS) Benefits are: (1) Create a single reliable source for facility data; (2) Improve transparency with oversight organizations; (3) Streamline FMOC business processes with a single, integrated facility-management tool; (4) Give customers simple tools and real-time information; (5) Reduce indirect costs; (6) Replace approximately 30 FMOC systems and 60 homegrown tools (such as Microsoft Access databases); and (7) Integrate with FIMS.« less

Hydrophobic Motif Phosphorylation Coordinates Activity and Polar Localization of the Neurospora crassa Nuclear Dbf2-Related Kinase COT1

PubMed Central

Maerz, Sabine; Dettmann, Anne

2012-01-01

Nuclear Dbf2p-related (NDR) kinases and associated proteins are recognized as a conserved network that regulates eukaryotic cell polarity. NDR kinases require association with MOB adaptor proteins and phosphorylation of two conserved residues in the activation segment and hydrophobic motif for activity and function. We demonstrate that the Neurospora crassa NDR kinase COT1 forms inactive dimers via a conserved N-terminal extension, which is also required for the interaction of the kinase with MOB2 to generate heterocomplexes with basal activity. Basal kinase activity also requires autophosphorylation of the COT1-MOB2 complex in the activation segment, while hydrophobic motif phosphorylation of COT1 by the germinal center kinase POD6 fully activates COT1 through induction of a conformational change. Hydrophobic motif phosphorylation is also required for plasma membrane association of the COT1-MOB2 complex. MOB2 further restricts the membrane-associated kinase complex to the hyphal apex to promote polar cell growth. These data support an integrated mechanism of NDR kinase regulation in vivo, in which kinase activation and cellular localization of COT1 are coordinated by dual phosphorylation and interaction with MOB2. PMID:22451488

Software Users Manual (SUM): Extended Testability Analysis (ETA) Tool

NASA Technical Reports Server (NTRS)

Maul, William A.; Fulton, Christopher E.

2011-01-01

This software user manual describes the implementation and use the Extended Testability Analysis (ETA) Tool. The ETA Tool is a software program that augments the analysis and reporting capabilities of a commercial-off-the-shelf (COTS) testability analysis software package called the Testability Engineering And Maintenance System (TEAMS) Designer. An initial diagnostic assessment is performed by the TEAMS Designer software using a qualitative, directed-graph model of the system being analyzed. The ETA Tool utilizes system design information captured within the diagnostic model and testability analysis output from the TEAMS Designer software to create a series of six reports for various system engineering needs. The ETA Tool allows the user to perform additional studies on the testability analysis results by determining the detection sensitivity to the loss of certain sensors or tests. The ETA Tool was developed to support design and development of the NASA Ares I Crew Launch Vehicle. The diagnostic analysis provided by the ETA Tool was proven to be valuable system engineering output that provided consistency in the verification of system engineering requirements. This software user manual provides a description of each output report generated by the ETA Tool. The manual also describes the example diagnostic model and supporting documentation - also provided with the ETA Tool software release package - that were used to generate the reports presented in the manual

External Dependencies-Driven Architecture Discovery and Analysis of Implemented Systems

NASA Technical Reports Server (NTRS)

Ganesan, Dharmalingam; Lindvall, Mikael; Ron, Monica

2014-01-01

A method for architecture discovery and analysis of implemented systems (AIS) is disclosed. The premise of the method is that architecture decisions are inspired and influenced by the external entities that the software system makes use of. Examples of such external entities are COTS components, frameworks, and ultimately even the programming language itself and its libraries. Traces of these architecture decisions can thus be found in the implemented software and is manifested in the way software systems use such external entities. While this fact is often ignored in contemporary reverse engineering methods, the AIS method actively leverages and makes use of the dependencies to external entities as a starting point for the architecture discovery. The AIS method is demonstrated using the NASA's Space Network Access System (SNAS). The results show that, with abundant evidence, the method offers reusable and repeatable guidelines for discovering the architecture and locating potential risks (e.g. low testability, decreased performance) that are hidden deep in the implementation. The analysis is conducted by using external dependencies to identify, classify and review a minimal set of key source code files. Given the benefits of analyzing external dependencies as a way to discover architectures, it is argued that external dependencies deserve to be treated as first-class citizens during reverse engineering. The current structure of a knowledge base of external entities and analysis questions with strategies for getting answers is also discussed.

International Cursor on Target (CoT) User Group Meeting (1st), 2-3 April 2013

DTIC Science & Technology

2013-07-01

0900 - 0930 BREAK 0930 - 0945 ATAK ( Android Tactical Assault Kit) Josh Sterling 0945 - 1045 Use of CoT in Military Operations Luke Savoie...satisfaction = 4; Food services = 5 Definitely poll for requirements requests ahead of time. Better multimedia system setup (though you did have a...really cool setup and multimedia thing!) Give a list of attendees to all so I can network. CoT and BAO Kit. Field incompatibility between CoT and

Simulation of Lunar Surface Communications Network Exploration Scenarios

NASA Technical Reports Server (NTRS)

Linsky, Thomas W.; Bhasin, Kul B.; White, Alex; Palangala, Srihari

2006-01-01

Simulations and modeling of surface-based communications networks provides a rapid and cost effective means of requirement analysis, protocol assessments, and tradeoff studies. Robust testing in especially important for exploration systems, where the cost of deployment is high and systems cannot be easily replaced or repaired. However, simulation of the envisioned exploration networks cannot be achieved using commercial off the shelf network simulation software. Models for the nonstandard, non-COTS protocols used aboard space systems are not readily available. This paper will address the simulation of realistic scenarios representative of the activities which will take place on the surface of the Moon, including selection of candidate network architectures, and the development of an integrated simulation tool using OPNET modeler capable of faithfully modeling those communications scenarios in the variable delay, dynamic surface environments. Scenarios for exploration missions, OPNET development, limitations, and simulations results will be provided and discussed.

Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions

PubMed Central

Hubbard, Kyle; Pellar, Gregory; Emanuel, Peter

2011-01-01

There is extensive data to support the use of commercial transport media as a stabilizer for known clinical samples; however, there is little information to support their use outside of controlled conditions specified by the manufacturer. Furthermore, there is no data to determine the suitability of said media for biological pathogens, specifically those of interest to the US military. This study evaluates commercial off-the-shelf (COTS) transport media based on sample recovery, viability, and quality of nucleic acids and peptides for nonpathogenic strains of Bacillus anthracis, Yersinia pestis, and Venezuelan equine encephalitis virus, in addition to ricin toxin. Samples were stored in COTS, PBST, or no media at various temperatures over an extended test period. The results demonstrate that COTS media, although sufficient for the preservation of nucleic acid and proteinaceous material, are not capable of maintaining an accurate representation of biothreat agents at the time of collection. PMID:22121364

A TinyOS-enabled MICA2-based wireless neural interface.

PubMed

Farshchi, Shahin; Nuyujukian, Paul H; Pesterev, Aleksey; Mody, Istvan; Judy, Jack W

2006-07-01

Existing approaches used to develop compact low-power multichannel wireless neural recording systems range from creating custom-integrated circuits to assembling commercial-off-the-shelf (COTS) PC-based components. Custom-integrated-circuit designs yield extremely compact and low-power devices at the expense of high development and upgrade costs and turn-around times, while assembling COTS-PC-technology yields high performance at the expense of large system size and increased power consumption. To achieve a balance between implementing an ultra-compact custom-fabricated neural transceiver and assembling COTS-PC-technology, an overlay of a neural interface upon the TinyOS-based MICA2 platform is described. The system amplifies, digitally encodes, and transmits neural signals real-time at a rate of 9.6 kbps, while consuming less than 66 mW of power. The neural signals are received and forwarded to a client PC over a serial connection. This data rate can be divided for recording on up to 6 channels, with a resolution of 8 bits/sample. This work demonstrates the strengths and limitations of the TinyOS-based sensor technology as a foundation for chronic remote biological monitoring applications and, thus, provides an opportunity to create a system that can leverage from the frequent networking and communications advancements being made by the global TinyOS-development community.

Spatial 3D infrastructure: display-independent software framework, high-speed rendering electronics, and several new displays

NASA Astrophysics Data System (ADS)

Chun, Won-Suk; Napoli, Joshua; Cossairt, Oliver S.; Dorval, Rick K.; Hall, Deirdre M.; Purtell, Thomas J., II; Schooler, James F.; Banker, Yigal; Favalora, Gregg E.

2005-03-01

We present a software and hardware foundation to enable the rapid adoption of 3-D displays. Different 3-D displays - such as multiplanar, multiview, and electroholographic displays - naturally require different rendering methods. The adoption of these displays in the marketplace will be accelerated by a common software framework. The authors designed the SpatialGL API, a new rendering framework that unifies these display methods under one interface. SpatialGL enables complementary visualization assets to coexist through a uniform infrastructure. Also, SpatialGL supports legacy interfaces such as the OpenGL API. The authors" first implementation of SpatialGL uses multiview and multislice rendering algorithms to exploit the performance of modern graphics processing units (GPUs) to enable real-time visualization of 3-D graphics from medical imaging, oil & gas exploration, and homeland security. At the time of writing, SpatialGL runs on COTS workstations (both Windows and Linux) and on Actuality"s high-performance embedded computational engine that couples an NVIDIA GeForce 6800 Ultra GPU, an AMD Athlon 64 processor, and a proprietary, high-speed, programmable volumetric frame buffer that interfaces to a 1024 x 768 x 3 digital projector. Progress is illustrated using an off-the-shelf multiview display, Actuality"s multiplanar Perspecta Spatial 3D System, and an experimental multiview display. The experimental display is a quasi-holographic view-sequential system that generates aerial imagery measuring 30 mm x 25 mm x 25 mm, providing 198 horizontal views.

Discovery of Imidazoquinolines as a Novel Class of Potent, Selective, and in Vivo Efficacious Cancer Osaka Thyroid (COT) Kinase Inhibitors.

PubMed

Glatthar, Ralf; Stojanovic, Aleksandar; Troxler, Thomas; Mattes, Henri; Möbitz, Henrik; Beerli, Rene; Blanz, Joachim; Gassmann, Ernst; Drückes, Peter; Fendrich, Gabriele; Gutmann, Sascha; Martiny-Baron, Georg; Spence, Fiona; Hornfeld, Jeff; Peel, John Edmonson; Sparrer, Helmut

2016-08-25

Cancer Osaka thyroid (COT) kinase is an important regulator of pro-inflammatory cytokines in macrophages. Thus, pharmacologic inhibition of COT should be a valid approach to therapeutically intervene in the pathogenesis of macrophage-driven inflammatory diseases such as rheumatoid arthritis. We report the discovery and chemical optimization of a novel series of COT kinase inhibitors, with unprecedented nanomolar potency for the inhibition of TNFα. Pharmacological profiling in vivo revealed a high metabolism of these compounds in rats which was demonstrated to be predominantly attributed to aldehyde oxidase. Due to the very low activity of hepatic AO in the dog, the selected candidate 32 displayed significant blood exposure in dogs which resulted in a clear prevention of inflammation-driven lameness. Taken together, the described compounds both potently and selectively inhibit COT kinase in primary human cells and ameliorate inflammatory pathologies in vivo, supporting the notion that COT is an appropriate therapeutic target for inflammatory diseases.

Plug-and-play design approach to smart harness for modular small satellites

NASA Astrophysics Data System (ADS)

Mughal, M. Rizwan; Ali, Anwar; Reyneri, Leonardo M.

2014-02-01

A typical satellite involves many different components that vary in bandwidth demand. Sensors that require a very low data rate may reside on a simple two- or three-wire interface such as I2C, SPI, etc. Complex sensors that require high data rate and bandwidth may reside on an optical interface. The AraMiS architecture is an enhanced capability architecture with different satellite configurations. Although keeping the low-cost and COTS approach of CubeSats, it extends the modularity concept as it also targets different satellite shapes and sizes. But modularity moves beyond the mechanical structure: the tiles also have thermo-mechanical, harness and signal-processing functionalities. Further modularizing the system, every tile can also host a variable number of small sensors, actuators or payloads, connected using a plug-and-play approach. Every subsystem is housed in a small daughter board and is supplied, by the main tile, with power and data distribution functions, power and data harness, mechanical support and is attached and interconnected with space-grade spring-loaded connectors. The tile software is also modular and allows a quick adaptation to specific subsystems. The basic software for the CPU is properly hardened to guarantee high level of radiation tolerance at very low cost.

Theoretical studies on structural, magnetic, and spintronic characteristics of sandwiched Eu(n)COT(n+1) (n = 1-4) clusters.

PubMed

Zhang, Xiuyun; Ng, Man-Fai; Wang, Yanbiao; Wang, Jinlan; Yang, Shuo-Wang

2009-09-22

Europium (Eu)-cyclootetatrene (COT = C(8)H(8)) multidecker clusters (Eu(n)COT(n+1), n = 1-4) are studied by relativistic density functional theory calculations. These clusters are found to be thermodynamically stable with freely rotatable COT rings, and their total magnetic moments (MMs) increase linearly along with the number of Eu atoms. Each Eu atom contributes about 7 mu(B) to the cluster. Meanwhile, the internal COT rings have little MM contribution while the external COT rings have about 1 mu(B) MM aligned in opposite direction to that of the Eu atoms. The total MM of the Eu(n)COT(n+1) clusters can thus be generalized as 7n - 2 mu(B) where n is the number of Eu atoms. Besides, the ground states of these clusters are ferromagnetic and energetically competitive with the antiferromagnetic states, meaning that their spin states are very unstable, especially for larger clusters. More importantly, we uncover an interesting bonding characteristic of these clusters in which the interior ionic structure is capped by two hybrid covalent-ionic terminals. We suggest that such a characteristic makes the Eu(n)COT(n+1) clusters extremely stable. Finally, we reveal that for the positively charged clusters, the hybrid covalent-ionic terminals will tip further toward the interior part of the clusters to form deeper covalent-ionic caps. In contrast, the negatively charged clusters turn to pure ionic structures.

48 CFR 225.7003-3 - Exceptions.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., subsystems, assemblies, or components. Specialty metal supply contracts issued by COTS producers are not... forgings or castings are incorporated into COTS end items, subsystems, or assemblies; (C) Commercially... incorporated into COTS end items or subsystems (see PGI 225.7003-3(b)(6) for a table of applicability of...

48 CFR 225.7003-3 - Exceptions.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., subsystems, assemblies, or components. Specialty metal supply contracts issued by COTS producers are not... forgings or castings are incorporated into COTS end items, subsystems, or assemblies; (C) Commercially... incorporated into COTS end items or subsystems (see PGI 225.7003-3(b)(6) for a table of applicability of...

48 CFR 225.7003-3 - Exceptions.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., subsystems, assemblies, or components. Specialty metal supply contracts issued by COTS producers are not... forgings or castings are incorporated into COTS end items, subsystems, or assemblies; (C) Commercially... incorporated into COTS end items or subsystems (see PGI 225.7003-3(b)(6) for a table of applicability of...

Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp (Ctenopharyngodon idellus): Induces immune responses and has no damage on liver and intestine function.

PubMed

Tang, Zeli; Sun, Hengchang; Chen, TingJin; Lin, Zhipeng; Jiang, Hongye; Zhou, Xinyi; Shi, Cunbin; Pan, Houjun; Chang, Ouqin; Ren, Pengli; Yu, Jinyun; Li, Xuerong; Xu, Jin; Huang, Yan; Yu, Xinbing

2017-05-01

Clonorchis sinensis (C. sinensis) is a fish-borne trematode. Human can be infected by ingestion of C. sinensis metacercariae parasitized in grass carp (Ctenopharyngodon idella). For induction of effective oral immune responses, spores of Bacillus subtilis (B. subtilis) WB600 were utilized as vehicle to delivery CsCP (cysteine protease of C. sinensis) cooperated with CotC (B.s-CotC-CP), one of coat proteins, to the gastrointestinal tract. After routine culture of 8-12 h in LB medium, B. subtilis containing CotC-CsCP was transferred into the sporulation culture medium. SDS-PAGE, western blotting and the growth curve indicated that the best sporulation time of recombinant WB600 was 24-30 h at 37 °C with continuous shaking (250 rpm). Grass carp were fed with three levels of B.s-CotC-CP (1 × 10 6 , 1 × 10 7 , and 1 × 10 8  CFU g -1 ) incorporated in the basal pellets diet. The commercial pellets or supplemented with spores just expressing CotC (1 × 10 7  CFU g -1 ) were served as control diet. Our results showed that grass carp orally immunized with the feed-based B.s-CotC-CP developed a strong specific immune response with significantly (P < 0.05) higher levels of IgM in samples of serum, bile, mucus of surface and intestinal compared to the control groups. Abundant colonization spores expressing CsCP were found in hindgut that is conducive to absorption and presentation of antigen. Moreover, B. subtilis spores appeared to show no sign of toxicity or damage in grass carp. Our cercariae challenge experiments suggested that oral administration of spores expressing CsCP could develop an effective protection against C. sinensis in fish body. Therefore, this study demonstrated that the feed-based recombinant spores could trigger high levels of mucosal and humoral immunity, and would be a promising candidate vaccine against C. sinensis metacercariae formation in freshwater fish. Copyright © 2017 Elsevier Ltd. All rights reserved.

The design of visualization telemetry system based on camera module of the commercial smartphone

NASA Astrophysics Data System (ADS)

Wang, Chao; Ye, Zhao; Wu, Bin; Yin, Huan; Cao, Qipeng; Zhu, Jun

2017-09-01

Satellite telemetry is the vital indicators to estimate the performance of the satellite. The telemetry data, the threshold range and the variation tendency collected during the whole operational life of the satellite, can guide and evaluate the subsequent design of the satellite in the future. The rotational parts on the satellite (e.g. solar arrays, antennas and oscillating mirrors) affect collecting the solar energy and the other functions of the satellite. Visualization telemetries (pictures, video) are captured to interpret the status of the satellite qualitatively in real time as an important supplement for troubleshooting. The mature technology of commercial off-the-shelf (COTS) products have obvious advantages in terms of the design of construction, electronics, interfaces and image processing. Also considering the weight, power consumption, and cost, it can be directly used in our application or can be adopted for secondary development. In this paper, characteristic simulations of solar arrays radiation in orbit are presented, and a suitable camera module of certain commercial smartphone is adopted after the precise calculation and the product selection process. Considering the advantages of the COTS devices, which can solve both the fundamental and complicated satellite problems, this technique proposed is innovative to the project implementation in the future.

CotA, a Multicopper Oxidase from Bacillus pumilus WH4, Exhibits Manganese-Oxidase Activity

PubMed Central

Su, Jianmei; Bao, Peng; Bai, Tenglong; Deng, Lin; Wu, Hui; Liu, Fan; He, Jin

2013-01-01

Multicopper oxidases (MCOs) are a family of enzymes that use copper ions as cofactors to oxidize various substrates. Previous research has demonstrated that several MCOs such as MnxG, MofA and MoxA can act as putative Mn(II) oxidases. Meanwhile, the endospore coat protein CotA from Bacillus species has been confirmed as a typical MCO. To study the relationship between CotA and the Mn(II) oxidation, the cotA gene from a highly active Mn(II)-oxidizing strain Bacillus pumilus WH4 was cloned and overexpressed in Escherichia coli strain M15. The purified CotA contained approximately four copper atoms per molecule and showed spectroscopic properties typical of blue copper oxidases. Importantly, apart from the laccase activities, the CotA also displayed substantial Mn(II)-oxidase activities both in liquid culture system and native polyacrylamide gel electrophoresis. The optimum Mn(II) oxidase activity was obtained at 53°C in HEPES buffer (pH 8.0) supplemented with 0.8 mM CuCl2. Besides, the addition of o-phenanthroline and EDTA both led to a complete suppression of Mn(II)-oxidizing activity. The specific activity of purified CotA towards Mn(II) was 0.27 U/mg. The Km, Vmax and kcat values towards Mn(II) were 14.85±1.17 mM, 3.01×10−6±0.21 M·min−1 and 0.32±0.02 s−1, respectively. Moreover, the Mn(II)-oxidizing activity of the recombinant E. coli strain M15-pQE-cotA was significantly increased when cultured both in Mn-containing K liquid medium and on agar plates. After 7-day liquid cultivation, M15-pQE-cotA resulted in 18.2% removal of Mn(II) from the medium. Furthermore, the biogenic Mn oxides were clearly observed on the cell surfaces of M15-pQE-cotA by scanning electron microscopy. To our knowledge, this is the first report that provides the direct observation of Mn(II) oxidation with the heterologously expressed protein CotA, Therefore, this novel finding not only establishes the foundation for in-depth study of Mn(II) oxidation mechanisms, but also offers a potential biocatalyst for Mn(II) removal. PMID:23577125

Differences in infant and parent behaviors during routine bed sharing compared with cot sleeping in the home setting.

PubMed

Baddock, Sally A; Galland, Barbara C; Bolton, David P G; Williams, Sheila M; Taylor, Barry J

2006-05-01

To observe the behavior of infants sleeping in the natural physical environment of home, comparing the 2 different sleep practices of bed sharing and cot sleeping quantifying to factors that have been identified as potential risks or benefits. Forty routine bed-sharing infants, aged 5-27 weeks were matched for age and season of study with 40 routine cot-sleeping infants. Overnight video and physiologic data of bed-share infants and cot-sleep infants were recorded in the infants' own homes. Sleep time, sleep position, movements, feeding, blanket height, parental checks, and time out of the bed or cot were logged. The total sleep time was similar in both groups (bed-sharing median: 8.6 hours; cot-sleeping median: 8.2 hours). Bed-sharing infants spent most time in the side position (median: 5.7 hours, 66% of sleep time) and most commonly woke at the end of sleep in this position, whereas cot-sleeping infants most commonly slept supine (median: 7.5 hours, 100%) and woke at the end of sleep in the supine position. Prone sleep was uncommon in both groups. Head covering above the eyes occurred in 22 bed-sharing infants and 1 cot-sleeping infant. Five of these bed-sharing infants were head covered at final waking time, but the cot-sleeping infant was not. Bed-sharing parents looked at or touched their infant more often (median: 11 vs 4 times per night) but did not always fully wake to do so. Movement episodes were shorter in the bed-sharing group as was total movement time (37 vs 50 minutes respectively), whereas feeding was 3.7 times more frequent in the bed-sharing group than the cot-sleeping group. Bed-share infants without known risk factors for sudden infant death syndrome (SIDS) experience increased maternal touching and looking, increased breastfeeding, and faster and more frequent maternal responses. This high level of interaction is unlikely to occur if maternal arousal is impaired, for example, by alcohol or overtiredness. Increased head covering and side sleep position occur during bed-sharing, but whether these factors increase the risk of SIDS, as they do in cot sleeping, requires further investigation.

Handheld emissions detector (HED): overview and development

NASA Astrophysics Data System (ADS)

Valentino, George J.; Schimmel, David

2009-05-01

Nova Engineering, Cincinnati OH, a division of L-3 Communications (L-3 Nova), under the sponsorship of Program Manager Soldier Warrior (PM-SWAR), Fort Belvoir, VA, has developed a Soldier portable, light-weight, hand-held, geolocation sensor and processing system called the Handheld Emissions Detector (HED). The HED is a broadband custom receiver and processor that allows the user to easily sense, direction find, and locate a broad range of emitters in the user's surrounding area. Now in its second design iteration, the HED incorporates a set of COTS components that are complemented with L-3 Nova custom RF, power, digital, and mechanical components, plus custom embedded and application software. The HED user interfaces are designed to provide complex information in a readily-understandable form, thereby providing actionable results for operators. This paper provides, where possible, the top-level characteristics of the HED as well as the rationale behind its design philosophy along with its applications in both DOD and Commercial markets.

Robotic tape library system level testing at NSA: Present and planned

NASA Technical Reports Server (NTRS)

Shields, Michael F.

1994-01-01

In the present of declining Defense budgets, increased pressure has been placed on the DOD to utilize Commercial Off the Shelf (COTS) solutions to incrementally solve a wide variety of our computer processing requirements. With the rapid growth in processing power, significant expansion of high performance networking, and the increased complexity of applications data sets, the requirement for high performance, large capacity, reliable and secure, and most of all affordable robotic tape storage libraries has greatly increased. Additionally, the migration to a heterogeneous, distributed computing environment has further complicated the problem. With today's open system compute servers approaching yesterday's supercomputer capabilities, the need for affordable, reliable secure Mass Storage Systems (MSS) has taken on an ever increasing importance to our processing center's ability to satisfy operational mission requirements. To that end, NSA has established an in-house capability to acquire, test, and evaluate COTS products. Its goal is to qualify a set of COTS MSS libraries, thereby achieving a modicum of standardization for robotic tape libraries which can satisfy our low, medium, and high performance file and volume serving requirements. In addition, NSA has established relations with other Government Agencies to complete this in-house effort and to maximize our research, testing, and evaluation work. While the preponderance of the effort is focused at the high end of the storage ladder, considerable effort will be extended this year and next at the server class or mid range storage systems.

Analytical Design Package (ADP2): A computer aided engineering tool for aircraft transparency design

NASA Technical Reports Server (NTRS)

Wuerer, J. E.; Gran, M.; Held, T. W.

1994-01-01

The Analytical Design Package (ADP2) is being developed as a part of the Air Force Frameless Transparency Program (FTP). ADP2 is an integrated design tool consisting of existing analysis codes and Computer Aided Engineering (CAE) software. The objective of the ADP2 is to develop and confirm an integrated design methodology for frameless transparencies, related aircraft interfaces, and their corresponding tooling. The application of this methodology will generate high confidence for achieving a qualified part prior to mold fabrication. ADP2 is a customized integration of analysis codes, CAE software, and material databases. The primary CAE integration tool for the ADP2 is P3/PATRAN, a commercial-off-the-shelf (COTS) software tool. The open architecture of P3/PATRAN allows customized installations with different applications modules for specific site requirements. Integration of material databases allows the engineer to select a material, and those material properties are automatically called into the relevant analysis code. The ADP2 materials database will be composed of four independent schemas: CAE Design, Processing, Testing, and Logistics Support. The design of ADP2 places major emphasis on the seamless integration of CAE and analysis modules with a single intuitive graphical interface. This tool is being designed to serve and be used by an entire project team, i.e., analysts, designers, materials experts, and managers. The final version of the software will be delivered to the Air Force in Jan. 1994. The Analytical Design Package (ADP2) will then be ready for transfer to industry. The package will be capable of a wide range of design and manufacturing applications.

Cot/tpl2-MKK1/2-Erk1/2 controls mTORC1-mediated mRNA translation in Toll-like receptor–activated macrophages

PubMed Central

López-Pelaéz, Marta; Fumagalli, Stefano; Sanz, Carlos; Herrero, Clara; Guerra, Susana; Fernandez, Margarita; Alemany, Susana

2012-01-01

Cot/tpl2 is the only MAP3K that activates MKK1/2-Erk1/2 in Toll-like receptor–activated macrophages. Here we show that Cot/tpl2 regulates RSK, S6 ribosomal protein, and 4E-BP phosphorylation after stimulation of bone marrow–derived macrophages with lipopolysaccharide (LPS), poly I:C, or zymosan. The dissociation of the 4E-BP–eIF4E complex, a key event in the cap-dependent mRNA translation initiation, is dramatically reduced in LPS-stimulated Cot/tpl2-knockout (KO) macrophages versus LPS-stimulated wild-type (Wt) macrophages. Accordingly, after LPS activation, increased cap-dependent translation is observed in Wt macrophages but not in Cot/tpl2 KO macrophages. In agreement with these data, Cot/tpl2 increases the polysomal recruitment of the 5´ TOP eEF1α and eEF2 mRNAs, as well as of inflammatory mediator gene–encoding mRNAs, such as tumor necrosis factor α (TNFα), interleukin-6 (IL-6), and KC in LPS-stimulated macrophages. In addition, Cot/tpl2 deficiency also reduces total TNFα, IL-6, and KC mRNA expression in LPS-stimulated macrophages, which is concomitant with a decrease in their mRNA half-lives. Macrophages require rapid fine control of translation to provide an accurate and not self-damaging response to host infection, and our data show that Cot/tpl2 controls inflammatory mediator gene–encoding mRNA translation in Toll-like receptor–activated macrophages. PMID:22675026

MODIS comparisons with northeastern Pacific in situ stratocumulus microphysics

NASA Astrophysics Data System (ADS)

Noble, Stephen R.; Hudson, James G.

2015-08-01

Vertical sounding measurements within stratocumuli during two aircraft field campaigns, Marine Stratus/stratocumulus Experiment (MASE) and Physics of Stratocumulus Top (POST), are used to validate Moderate Resolution Imaging Spectroradiometer (MODIS) cloud optical thickness (COT), cloud liquid water path (LWP), and cloud effective radius (re). In situ COT, LWP, and re were calculated using 5 m vertically averaged droplet probe measurements of complete vertical cloud penetrations. MODIS COT, LWP, and re 1 km pixels were averaged along these penetrations. COT comparisons in POST showed strong correlations and a near 1:1 relationship. In MASE, comparisons showed strong correlations; however, MODIS COT exceeded in situ COT, likely due to larger temporal differences between MODIS and in situ measurements. LWP comparisons between two cloud probes show good agreement for POST but not MASE, giving confidence to POST data. Both projects provided strong LWP correlations but MODIS exceeded in situ by 14-36%. MODIS in situ re correlations were strong, but MODIS 2.1 µm re exceeded in situ re, which contributed to LWP bias; in POST, MODIS re was 20-30% greater than in situ re. Maximum in situ re near cloud top showed comparisons nearer 1:1. Other MODIS re bands (3.7 µm and 1.6 µm) showed similar comparisons. Temporal differences between MODIS and in situ measurements, airplane speed differences, and cloud probe artifacts were likely causes of weaker MASE correlations. POST COT comparison was best for temporal differences under 20 min. POST data validate MODIS COT but it also implies a positive MODIS re bias that propagates to LWP while still capturing variability.

Cot/tpl2 activity is required for TLR-induced activation of the Akt p70 S6k pathway in macrophages: Implications for NO synthase 2 expression.

PubMed

López-Peláez, Marta; Soria-Castro, Irene; Boscá, Lisardo; Fernández, Margarita; Alemany, Susana

2011-06-01

LPS stimulation activates IKK and different MAP kinase pathways, as well as the PI3K-Akt-mTOR-p70 S6k pathway, a negative regulator of these MyD88-dependent intracellular signals. Here, we show that Cot/tpl2, a MAP3K responsible for the activation of the MKK1-Erk1/2, controls P-Ser473 Akt and P-Thr389 p70 S6k phosphorylation in LPS-stimulated macrophages. Analysis of the intracellular signalling in Cot/tpl2 KO macrophages versus WT macrophages reveals lower IκBα recovery and higher phosphorylation of JNK and p38α after 1 h of LPS stimulation. Moreover, Cot/tpl2 deficiency increases LPS-induced NO synthase 2 (NOS2) expression in macrophages. Inhibition of the PI3K pathway abolishes the differences in IκBα and NOS2 expression between Cot/tpl2 KO and WT macrophages following LPS administration. Furthermore, in zymosan- and polyI:C-stimulated macrophages, Cot/tpl2 mediates P-Ser473 Akt phosphorylation, increases IκBα levels and decreases NOS2 expression. In conclusion, these data reveal a novel role for the Cot/tpl2 pathway in mediating TLR activation of the Akt-mTOR-p70 S6k pathway, allowing Cot/tpl2 to fine-control the activation state of other signalling pathways. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Cot/tpl2-MKK1/2-Erk1/2 controls mTORC1-mediated mRNA translation in Toll-like receptor-activated macrophages.

PubMed

López-Pelaéz, Marta; Fumagalli, Stefano; Sanz, Carlos; Herrero, Clara; Guerra, Susana; Fernandez, Margarita; Alemany, Susana

2012-08-01

Cot/tpl2 is the only MAP3K that activates MKK1/2-Erk1/2 in Toll-like receptor-activated macrophages. Here we show that Cot/tpl2 regulates RSK, S6 ribosomal protein, and 4E-BP phosphorylation after stimulation of bone marrow-derived macrophages with lipopolysaccharide (LPS), poly I:C, or zymosan. The dissociation of the 4E-BP-eIF4E complex, a key event in the cap-dependent mRNA translation initiation, is dramatically reduced in LPS-stimulated Cot/tpl2-knockout (KO) macrophages versus LPS-stimulated wild-type (Wt) macrophages. Accordingly, after LPS activation, increased cap-dependent translation is observed in Wt macrophages but not in Cot/tpl2 KO macrophages. In agreement with these data, Cot/tpl2 increases the polysomal recruitment of the 5´ TOP eEF1α and eEF2 mRNAs, as well as of inflammatory mediator gene-encoding mRNAs, such as tumor necrosis factor α (TNFα), interleukin-6 (IL-6), and KC in LPS-stimulated macrophages. In addition, Cot/tpl2 deficiency also reduces total TNFα, IL-6, and KC mRNA expression in LPS-stimulated macrophages, which is concomitant with a decrease in their mRNA half-lives. Macrophages require rapid fine control of translation to provide an accurate and not self-damaging response to host infection, and our data show that Cot/tpl2 controls inflammatory mediator gene-encoding mRNA translation in Toll-like receptor-activated macrophages.

48 CFR 12.103 - Commercially available off-the-shelf (COTS) items.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ACQUISITION REGULATION ACQUISITION PLANNING ACQUISITION OF COMMERCIAL ITEMS Acquisition of Commercial Items... indicated otherwise, all of the policies that apply to commercial items also apply to COTS. Section 12.505 lists the laws that are not applicable to COTS (in addition to 12.503 and 12.504); the components test...

Regulation of Neurospora crassa cell wall remodeling via the cot-1 pathway is mediated by gul-1.

PubMed

Herold, Inbal; Yarden, Oded

2017-02-01

Impairment of the Neurospora crassa Nuclear DBF2-related kinase-encoding gene cot-1 results in pleiotropic effects, including abnormally thick hyphal cell walls and septa. An increase in the transcript abundance of genes encoding chitin and glucan synthases and the chitinase gh18-5, but not the cell wall integrity pathway transcription factor rlm-1, accompany the phenotypic changes observed. Deletion of chs-5 or chs-7 in a cot-1 background results in a reduction of hyperbranching frequency characteristic of the cot-1 parent. gul-1 (a homologue of the yeast SSD1 gene) encodes a translational regulator and has been shown to partially suppress cot-1. We demonstrate that the high expression levels of the cell wall remodeling genes analyzed is curbed, and reaches near wild type levels, when gul-1 is inactivated. This is accompanied by morphological changes that include reduced cell wall thickness and restoration of normal chitin levels. We conclude that gul-1 is a mediator of cell wall remodeling within the cot-1 pathway.

Work Enabling Opioid Management.

PubMed

Lavin, Robert A; Kalia, Nimisha; Yuspeh, Larry; Barry, Jill A; Bernacki, Edward J; Tao, Xuguang Grant

2017-08-01

This study describes the relationship between opioid prescribing and ability to work. The opioid prescription patterns of 4994 claimants were studied. Three groups were constructed: 1) at least 3 consecutive months prescribed (chronic opioid therapy; COT); 2) less than 3 consecutive months prescribed (acute opioid therapy; AOT); and 3) no opioids prescribed. Variables included sex, age, daily morphine equivalent dose (MED), days opioids were prescribed, temporary total days (TTDs), and medical/indemnity/total costs. The COT versus AOT claimants had higher opioid costs ($8618 vs $94), longer TTD (636.2 vs 182.3), and average MED (66.8 vs 34.9). Only 2% of the COT cohort were not released to work. Fifty-seven percent of patients in the COT category (64 of 112) were released to work while still receiving opioids. COT does not preclude ability to work when prescribing within established guidelines.

Characterization of Uranium Ore Concentrate Chemical Composition via Raman Spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Su, Yin-Fong; Tonkyn, Russell G.; Sweet, Lucas E.

Uranium Ore Concentrate (UOC, often called yellowcake) is a generic term that describes the initial product resulting from the mining and subsequent milling of uranium ores en route to production of the U-compounds used in the fuel cycle. Depending on the mine, the ore, the chemical process, and the treatment parameters, UOC composition can vary greatly. With the recent advent of handheld spectrometers, we have chosen to investigate whether either commercial off-the-shelf (COTS) handheld devices or laboratory-grade Raman instruments might be able to i) identify UOC materials, and ii) differentiate UOC samples based on chemical composition and thus suggest themore » mining or milling process. Twenty-eight UOC samples were analyzed via FT-Raman spectroscopy using both 1064 nm and 785 nm excitation wavelengths. These data were also compared with results from a newly developed handheld COTS Raman spectrometer using a technique that lowers background fluorescence signal. Initial chemometric analysis was able to differentiate UOC samples based on mine location. Additional compositional information was obtained from the samples by performing XRD analysis on a subset of samples. The compositional information was integrated with chemometric analysis of the spectroscopic dataset allowing confirmation that class identification is possible based on compositional differences between the UOC samples, typically involving species such as U3O8, α-UO2(OH)2, UO4•2H2O (metastudtite), K(UO2)2O3, etc. While there are clearly excitation λ sensitivities, especially for dark samples, Raman analysis coupled with chemometric data treatment can nicely differentiate UOC samples based on composition and even mine origin.« less

Pushing the Limits of Cubesat Attitude Control: A Ground Demonstration

NASA Technical Reports Server (NTRS)

Sanders, Devon S.; Heater, Daniel L.; Peeples, Steven R.; Sules. James K.; Huang, Po-Hao Adam

2013-01-01

A cubesat attitude control system (ACS) was designed at the NASA Marshall Space Flight Center (MSFC) to provide sub-degree pointing capabilities using low cost, COTS attitude sensors, COTS miniature reaction wheels, and a developmental micro-propulsion system. The ACS sensors and actuators were integrated onto a 3D-printed plastic 3U cubesat breadboard (10 cm x 10 cm x 30 cm) with a custom designed instrument board and typical cubesat COTS hardware for the electrical, power, and data handling and processing systems. In addition to the cubesat development, a low-cost air bearing was designed and 3D printed in order to float the cubesat in the test environment. Systems integration and verification were performed at the MSFC Small Projects Rapid Integration & Test Environment laboratory. Using a combination of both the miniature reaction wheels and the micro-propulsion system, the open and closed loop control capabilities of the ACS were tested in the Flight Robotics Laboratory. The testing demonstrated the desired sub-degree pointing capability of the ACS and also revealed the challenges of creating a relevant environment for development testin

Sporulation Temperature Reveals a Requirement for CotE in the Assembly of both the Coat and Exosporium Layers of Bacillus cereus Spores.

PubMed

Bressuire-Isoard, Christelle; Bornard, Isabelle; Henriques, Adriano O; Carlin, Frédéric; Broussolle, Véronique

2016-01-01

The Bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. We investigated the interplay between the sporulation temperature and the CotE morphogenetic protein in the assembly of the surface layers of B. cereus ATCC 14579 spores and on the resulting spore properties. The cotE deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°C and at the optimal growth temperature of 37°C. Transmission electron microscopy revealed that ΔcotE spores had a fragmented and detached exosporium when formed at 37°C. However, when produced at 20°C, ΔcotE spores showed defects in both coat and exosporium attachment and were susceptible to lysozyme and mutanolysin. Thus, CotE has a role in the assembly of both the coat and exosporium, which is more important during sporulation at 20°C. CotE was more represented in extracts from spores formed at 20°C than at 37°C, suggesting that increased synthesis of the protein is required to maintain proper assembly of spore surface layers at the former temperature. ΔcotE spores formed at either sporulation temperature were impaired in inosine-triggered germination and resistance to UV-C and H2O2 and were less hydrophobic than wild-type (WT) spores but had a higher resistance to wet heat. While underscoring the role of CotE in the assembly of B. cereus spore surface layers, our study also suggests a contribution of the protein to functional properties of additional spore structures. Moreover, it also suggests a complex relationship between the function of a spore morphogenetic protein and environmental factors such as the temperature during spore formation. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Cot/tpl2 participates in the activation of macrophages by adiponectin.

PubMed

Sanz-Garcia, Carlos; Nagy, Laura E; Lasunción, Miguel A; Fernandez, Margarita; Alemany, Susana

2014-06-01

Whereas the main function of APN is to enhance insulin activity, it is also involved in modulating the macrophage phenotype. Here, we demonstrate that at physiological concentrations, APN activates Erk1/2 via the IKKβ-p105/NF-κΒ1-Cot/tpl2 intracellular signal transduction cassette in macrophages. In peritoneal macrophages stimulated with APN, Cot/tpl2 influences the ability to phagocytose beads. However, Cot/tpl2 did not modulate the known capacity of APN to decrease lipid content in peritoneal macrophages in response to treatment with oxLDL or acLDL. A microarray analysis of gene-expression profiles in BMDMs exposed to APN revealed that APN modulated the expression of ∼3300 genes; the most significantly affected biological functions were the inflammatory and the infectious disease responses. qRT-PCR analysis of WT and Cot/tpl2 KO macrophages stimulated with APN for 0, 3, and 18 h revealed that Cot/tpl2 participated in the up-regulation of APN target inflammatory mediators included in the cytokine-cytokine receptor interaction pathway (KEGG ID 4060). In accordance with these data, macrophages stimulated with APN increased secretion of cytokines and chemokines, including IL-1β, IL-1α, TNF-α, IL-10, IL-12, IL-6, and CCL2. Moreover, Cot/tpl2 also played an important role in the production of these inflammatory mediators upon stimulation of macrophages with APN. It has been reported that different types of signals that stimulate TLRs, IL-1R, TNFR, FcγR, and proteinase-activated receptor-1 activate Cot/tpl2. Here, we demonstrate that APN is a new signal that activates the IKKβ-p105/NF-κΒ1-Cot/tpl2-MKK1/2-Erk1/2 axis in macrophages. Furthermore, this signaling cassette modulates the biological functions triggered by APN in macrophages. © 2014 Society for Leukocyte Biology.

Cot/tpl2 participates in the activation of macrophages by adiponectin

PubMed Central

Sanz-Garcia, Carlos; Nagy, Laura E.; Lasunción, Miguel A.; Fernandez, Margarita; Alemany, Susana

2014-01-01

Whereas the main function of APN is to enhance insulin activity, it is also involved in modulating the macrophage phenotype. Here, we demonstrate that at physiological concentrations, APN activates Erk1/2 via the IKKβ-p105/NF-κΒ1-Cot/tpl2 intracellular signal transduction cassette in macrophages. In peritoneal macrophages stimulated with APN, Cot/tpl2 influences the ability to phagocytose beads. However, Cot/tpl2 did not modulate the known capacity of APN to decrease lipid content in peritoneal macrophages in response to treatment with oxLDL or acLDL. A microarray analysis of gene-expression profiles in BMDMs exposed to APN revealed that APN modulated the expression of ∼3300 genes; the most significantly affected biological functions were the inflammatory and the infectious disease responses. qRT-PCR analysis of WT and Cot/tpl2 KO macrophages stimulated with APN for 0, 3, and 18 h revealed that Cot/tpl2 participated in the up-regulation of APN target inflammatory mediators included in the cytokine–cytokine receptor interaction pathway (KEGG ID 4060). In accordance with these data, macrophages stimulated with APN increased secretion of cytokines and chemokines, including IL-1β, IL-1α, TNF-α, IL-10, IL-12, IL-6, and CCL2. Moreover, Cot/tpl2 also played an important role in the production of these inflammatory mediators upon stimulation of macrophages with APN. It has been reported that different types of signals that stimulate TLRs, IL-1R, TNFR, FcγR, and proteinase-activated receptor-1 activate Cot/tpl2. Here, we demonstrate that APN is a new signal that activates the IKKβ-p105/NF-κΒ1-Cot/tpl2-MKK1/2-Erk1/2 axis in macrophages. Furthermore, this signaling cassette modulates the biological functions triggered by APN in macrophages. PMID:24532642

Interleukin-33/ST2 axis promotes epithelial cell transformation and breast tumorigenesis via upregulation of COT activity.

PubMed

Kim, J Y; Lim, S-C; Kim, G; Yun, H J; Ahn, S-G; Choi, H S

2015-09-17

Cytokines of the interleukin-1 (IL-1) family, such as IL-1α/β and IL-18, have pleiotropic activities in innate and adaptive immune responses in host defense and diseases. Insight into their biological functions helped develop novel therapeutic approaches to treat human inflammatory diseases. IL-33 is an important member of the IL-1 family of cytokines and is a ligand of the ST2 receptor, a member of the IL-1 receptor family. However, the role of the IL-33/ST2 axis in tumor growth and metastasis of breast cancer remains unclear. Here, we demonstrate that IL-33 is a critical tumor promoter during epithelial cell proliferation and tumorigenesis in the breast. IL-33 dose- and time-dependently increased Cancer Osaka Thyroid (COT) phosphorylation via ST2-COT interaction in normal epithelial and breast cancer cells. The IL-33/ST2/COT cascade induced the activation of the MEK-ERK (MEK-extracellular signal-regulated kinase), JNK-cJun (cJun N-terminal kinase-cJun) and STAT3 (signal transducer and activator of transcription 3) signaling pathways, followed by increased AP-1 and stat3 transcriptional activity. When small interfering RNAs of ST2 and COT were introduced into cells, IL-33-induced AP-1 and stat3 activity were significantly decreased, unlike that in the control cells. The inhibition of COT activity resulted in decreased IL-33-induced epithelial cell transformation, and knockdown of IL-33, ST2 and COT in breast cancer cells attenuated tumorigenicity of breast cancer cells. Consistent with these observations, ST2 levels were positively correlated with COT expression in human breast cancer. These findings provide a novel perspective on the role of the IL-33/ST2/COT signaling pathway in supporting cancer-associated inflammation in the tumor microenvironment. Therapeutic approaches that target this pathway may, therefore, effectively inhibit carcinogenesis in the breast.

Architecture and assembly of the Bacillus subtilis spore coat.

PubMed

Plomp, Marco; Carroll, Alicia Monroe; Setlow, Peter; Malkin, Alexander J

2014-01-01

Bacillus spores are encased in a multilayer, proteinaceous self-assembled coat structure that assists in protecting the bacterial genome from stresses and consists of at least 70 proteins. The elucidation of Bacillus spore coat assembly, architecture, and function is critical to determining mechanisms of spore pathogenesis, environmental resistance, immune response, and physicochemical properties. Recently, genetic, biochemical and microscopy methods have provided new insight into spore coat architecture, assembly, structure and function. However, detailed spore coat architecture and assembly, comprehensive understanding of the proteomic composition of coat layers, and specific roles of coat proteins in coat assembly and their precise localization within the coat remain in question. In this study, atomic force microscopy was used to probe the coat structure of Bacillus subtilis wild type and cotA, cotB, safA, cotH, cotO, cotE, gerE, and cotE gerE spores. This approach provided high-resolution visualization of the various spore coat structures, new insight into the function of specific coat proteins, and enabled the development of a detailed model of spore coat architecture. This model is consistent with a recently reported four-layer coat assembly and further adds several coat layers not reported previously. The coat is organized starting from the outside into an outermost amorphous (crust) layer, a rodlet layer, a honeycomb layer, a fibrous layer, a layer of "nanodot" particles, a multilayer assembly, and finally the undercoat/basement layer. We propose that the assembly of the previously unreported fibrous layer, which we link to the darkly stained outer coat seen by electron microscopy, and the nanodot layer are cotH- and cotE- dependent and cotE-specific respectively. We further propose that the inner coat multilayer structure is crystalline with its apparent two-dimensional (2D) nuclei being the first example of a non-mineral 2D nucleation crystallization pattern in a biological organism.

Architecture and Assembly of the Bacillus subtilis Spore Coat

PubMed Central

Plomp, Marco; Carroll, Alicia Monroe; Setlow, Peter; Malkin, Alexander J.

2014-01-01

Bacillus spores are encased in a multilayer, proteinaceous self-assembled coat structure that assists in protecting the bacterial genome from stresses and consists of at least 70 proteins. The elucidation of Bacillus spore coat assembly, architecture, and function is critical to determining mechanisms of spore pathogenesis, environmental resistance, immune response, and physicochemical properties. Recently, genetic, biochemical and microscopy methods have provided new insight into spore coat architecture, assembly, structure and function. However, detailed spore coat architecture and assembly, comprehensive understanding of the proteomic composition of coat layers, and specific roles of coat proteins in coat assembly and their precise localization within the coat remain in question. In this study, atomic force microscopy was used to probe the coat structure of Bacillus subtilis wild type and cotA, cotB, safA, cotH, cotO, cotE, gerE, and cotE gerE spores. This approach provided high-resolution visualization of the various spore coat structures, new insight into the function of specific coat proteins, and enabled the development of a detailed model of spore coat architecture. This model is consistent with a recently reported four-layer coat assembly and further adds several coat layers not reported previously. The coat is organized starting from the outside into an outermost amorphous (crust) layer, a rodlet layer, a honeycomb layer, a fibrous layer, a layer of “nanodot” particles, a multilayer assembly, and finally the undercoat/basement layer. We propose that the assembly of the previously unreported fibrous layer, which we link to the darkly stained outer coat seen by electron microscopy, and the nanodot layer are cotH- and cotE- dependent and cotE-specific respectively. We further propose that the inner coat multilayer structure is crystalline with its apparent two-dimensional (2D) nuclei being the first example of a non-mineral 2D nucleation crystallization pattern in a biological organism. PMID:25259857

Spectral mapping tools from the earth sciences applied to spectral microscopy data.

PubMed

Harris, A Thomas

2006-08-01

Spectral imaging, originating from the field of earth remote sensing, is a powerful tool that is being increasingly used in a wide variety of applications for material identification. Several workers have used techniques like linear spectral unmixing (LSU) to discriminate materials in images derived from spectral microscopy. However, many spectral analysis algorithms rely on assumptions that are often violated in microscopy applications. This study explores algorithms originally developed as improvements on early earth imaging techniques that can be easily translated for use with spectral microscopy. To best demonstrate the application of earth remote sensing spectral analysis tools to spectral microscopy data, earth imaging software was used to analyze data acquired with a Leica confocal microscope with mechanical spectral scanning. For this study, spectral training signatures (often referred to as endmembers) were selected with the ENVI (ITT Visual Information Solutions, Boulder, CO) "spectral hourglass" processing flow, a series of tools that use the spectrally over-determined nature of hyperspectral data to find the most spectrally pure (or spectrally unique) pixels within the data set. This set of endmember signatures was then used in the full range of mapping algorithms available in ENVI to determine locations, and in some cases subpixel abundances of endmembers. Mapping and abundance images showed a broad agreement between the spectral analysis algorithms, supported through visual assessment of output classification images and through statistical analysis of the distribution of pixels within each endmember class. The powerful spectral analysis algorithms available in COTS software, the result of decades of research in earth imaging, are easily translated to new sources of spectral data. Although the scale between earth imagery and spectral microscopy is radically different, the problem is the same: mapping material locations and abundances based on unique spectral signatures. (c) 2006 International Society for Analytical Cytology.

Tpl-2/Cot and COX-2 in breast cancer.

PubMed

Krcova, Zuzana; Ehrmann, Jiri; Krejci, Veronika; Eliopoulos, Aris; Kolar, Zdenek

2008-06-01

Breast cancer is the most common cancer in women worldwide and although mortality (129,000/year) stagnates, incidence (370,000/year) is increasing. In addition to histological type, grade, stage, hormonal and c-erbB2 status there is therefore a strong need for new and reliable prognostic and predictive factors. This minireview focuses on two potential prognostic and predictive candidates Tpl2/Cot and COX-2 and summarise information about them. Tumor progression locus 2 (Tpl2/Cot) is a serine/threonine protein kinase belonging to the family of MAP3 kinases. Activated Tpl2/Cot leads to induction of ERK1/2, JNK, NF-kappaB and p38MAPK pathways. The first study on Tpl2/Cot mRNA in breast cancer showed its increase in 40 % of cases of breast cancer but no available data exist on protein expression. Cyclo-oxygenase 2 (COX-2) is inducible by growth and inflammatory factors and contributes to the development of various tumours. Expression of COX-2 in breast cancer varied from 5-100 % in reviewed papers with significantly higher values in poorly differentiated tumours. Tpl2/Cot and COX-2 have their importance in different intracellular pathways and some of these are involved in cancer development. Briefly, the results from recent studies suggest that Tpl2/Cot and COX-2 could be prognostic factors in breast cancer.

Validation of a "Kane's Dynamics" Model for the Active Rack Isolation System

NASA Technical Reports Server (NTRS)

Beech, Geoffrey S.; Hampton, R. David

2000-01-01

Many microgravity space-science experiments require vibratory acceleration levels unachievable without active isolation. The Boeing Corporation's Active Rack Isolation System (ARIS) employs a novel combination of magnetic actuation and mechanical linkages, to address these isolation requirements on the International Space Station (ISS). ARIS provides isolation at the rack (international Standard Payload Rack, or ISPR) level. Effective model-based vibration isolation requires (1) an isolation device, (2) an adequate dynamic (i.e., mathematical) model of that isolator, and (3) a suitable, corresponding controller, ARIS provides the ISS response to the first requirement. In November 1999, the authors presented a response to the second ("A 'Kane's Dynamics' model for the Active Rack Isolation System", Hampton and Beech) intended to facilitate an optimal-controls approach to the third. This paper documents the validation of that high-fidelity dynamic model of ARIS. As before, this model contains the full actuator dynamics, however, the umbilical models are not included in this presentation. The validation of this dynamics model was achieved by utilizing two Commercial Off the Shelf (COTS) software tools: Deneb's ENVISION, and Online Dynamics' AUTOLEV. ENVISION is a robotics software package developed for the automotive industry that employs 3-dimensional (3-D) Computer Aided Design (CAD) models to facilitate both forward and inverse kinematics analyses. AUTOLEV is a DOS based interpreter that is designed in general to solve vector based mathematical problems and specifically to solve Dynamics problems using Kane's method.

Altering Neurospora crassa MOB2A exposes its functions in development and affects its interaction with the NDR kinase COT1.

PubMed

Aharoni-Kats, Liran; Zelinger, Einat; Chen, She; Yarden, Oded

2018-06-01

The Neurospora crassa Mps One Binder (MOB) proteins MOB2A and MOB2B physically interact with the Nuclear Dbf2 Related (NDR) kinase COT1 and have been shown to have overlapping functions in various aspects of asexual development. Here, we identified two N. crassa MOB2A residues, Tyr117 and Tyr119, which are potentially phosphorylated. Using phosphomimetic mob-2a mutants we have been able to establish that apart from their previously described roles, MOB2A/B are involved in additional developmental processes. Enhanced conidial germination, accompanied by conidial agglutination, in the phosphomimetic mutants indicated that MOB2A is a negative regulator of germination. Thick-section imaging of perithecia revealed slow maturation and a lack of asci alignment in the mutant strains demonstrating a role for MOB2A in sexual development. We demonstrate that even though MOB2A and MOB2B have some overlapping functions, MOB2B cannot compensate for the roles MOB2A has in conidiation and germination. Altering Tyr residues 117 and 119 impaired the physical interactions between MOB2A and COT1, most likely contributing to some of the observed effects. As cot-1 and the phosphomimetic mutants share an extragenic suppressor (gul-1), we concluded that at least some of the effects imposed by altering Tyr117 and Tyr119 are mediated by the NDR kinase. © 2018 John Wiley & Sons Ltd.

Nicotine Metabolite Ratio (3-hydroxycotinine/cotinine) in Plasma and Urine by Different Analytical Methods and Laboratories: Implications for Clinical Implementation

PubMed Central

Tanner, Julie-Anne; Novalen, Maria; Jatlow, Peter; Huestis, Marilyn A.; Murphy, Sharon E.; Kaprio, Jaakko; Kankaanpää, Aino; Galanti, Laurence; Stefan, Cristiana; George, Tony P.; Benowitz, Neal L.; Lerman, Caryn; Tyndale, Rachel F.

2015-01-01

Background The highly genetically variable enzyme CYP2A6 metabolizes nicotine to cotinine (COT) and COT to trans-3′-hydroxycotinine (3HC). The nicotine metabolite ratio (NMR, 3HC/COT) is commonly used as a biomarker of CYP2A6 enzymatic activity, rate of nicotine metabolism, and total nicotine clearance; NMR is associated with numerous smoking phenotypes, including smoking cessation. Our objective was to investigate the impact of different measurement methods, at different sites, on plasma and urinary NMR measures from ad libitum smokers. Methods Plasma (n=35) and urine (n=35) samples were sent to eight different laboratories, which employed similar and different methods of COT and 3HC measurements to derive the NMR. We used Bland-Altman analysis to assess agreement, and Pearson correlations to evaluate associations, between NMR measured by different methods. Results Measures of plasma NMR were in strong agreement between methods according to Bland-Altman analysis (ratios 0.82–1.16) and were highly correlated (all Pearson r>0.96, P<0.0001). Measures of urinary NMR were in relatively weaker agreement (ratios 0.62–1.71) and less strongly correlated (Pearson r values of 0.66–0.98, P<0.0001) between different methods. Plasma and urinary COT and 3HC concentrations, while weaker than NMR, also showed good agreement in plasma, which was better than in urine, as was observed for NMR. Conclusions Plasma is a very reliable biological source for the determination of NMR, robust to differences in these analytical protocols or assessment site. Impact Together this indicates a reduced need for differential interpretation of plasma NMR results based on the approach used, allowing for direct comparison of different studies. PMID:26014804

Observational study of treatment space in individual neonatal cot spaces.

PubMed

Hignett, Sue; Lu, Jun; Fray, Mike

2010-01-01

Technology developments in neonatal intensive care units have increased the spatial requirements for clinical activities. Because the effectiveness of healthcare delivery is determined in part by the design of the physical environment and the spatial organization of work, it is appropriate to apply an evidence-based approach to architectural design. This study aimed to provide empirical evidence of the spatial requirements for an individual cot or incubator space. Observational data from 2 simulation exercises were combined with an expert review to produce a final recommendation. A validated 5-step protocol was used to collect data. Step 1 defined the clinical specialty and space. In step 2, data were collected with 28 staff members and 15 neonates to produce a simulation scenario representing the frequent and safety-critical activities. In step 3, 21 staff members participated in functional space experiments to determine the average spatial requirements. Step 4 incorporated additional data (eg, storage and circulation) to produce a spatial recommendation. Finally, the recommendation was reviewed in step 5 by a national expert clinical panel to consider alternative layouts and technology. The average space requirement for an individual neonatal intensive care unit cot (incubator) space was 13.5 m2 (or 145.3 ft2). The circulation and storage space requirements added in step 4 increased this to 18.46 m2 (or 198.7 ft2). The expert panel reviewed the recommendation and agreed that the average individual cot space (13.5 m2/[or 145.3 ft2]) would accommodate variance in working practices. Care needs to be taken when extrapolating this recommendation to multiple cot areas to maintain the minimum spatial requirement.

Assessment of the NPOESS/VIIRS Nighttime Infrared Cloud Optical Properties Algorithms

NASA Astrophysics Data System (ADS)

Wong, E.; Ou, S. C.

2008-12-01

In this paper we will describe two NPOESS VIIRS IR algorithms used to retrieve microphysical properties for water and ice clouds during nighttime conditions. Both algorithms employ four VIIRS IR channels: M12 (3.7 μm), M14 (8.55 μm), M15 (10.7 μm) and M16 (12 μm). The physical basis for the two algorithms is similar in that while the Cloud Top Temperature (CTT) is derived from M14 and M16 for ice clouds the Cloud Optical Thickness (COT) and Cloud Effective Particle Size (CEPS) are derived from M12 and M15. The two algorithms depart in the different radiative transfer parameterization equations used for ice and water clouds. Both the VIIRS nighttime IR algorithms and the CERES split-window method employ the 3.7 μm and 10.7 μm bands for cloud optical properties retrievals, apparently based on similar physical principles but with different implementations. It is reasonable to expect that the VIIRS and CERES IR algorithms produce comparable performance and similar limitations. To demonstrate the VIIRS nighttime IR algorithm performance, we will select a number of test cases using NASA MODIS L1b radiance products as proxy input data for VIIRS. The VIIRS retrieved COT and CEPS will then be compared to cloud products available from the MODIS, NASA CALIPSO, CloudSat and CERES sensors. For the MODIS product, the nighttime cloud emissivity will serve as an indirect comparison to VIIRS COT. For the CALIPSO and CloudSat products, the layered COT will be used for direct comparison. Finally, the CERES products will provide direct comparison with COT as well as CEPS. This study can only provide a qualitative assessment of the VIIRS IR algorithms due to the large uncertainties in these cloud products.

Survey of American College of Surgeons Committee on trauma members on firearm injury: Consensus and opportunities.

PubMed

Kuhls, Deborah A; Campbell, Brendan T; Burke, Peter A; Allee, Lisa; Hink, Ashley; Letton, Robert W; Masiakos, Peter T; Coburn, Michael; Alvi, Maria; Lerer, Trudy J; Gaines, Barbara A; Nance, Michael L; Schuerer, Douglas J E; Palmieri, Tina L; Davis, James W; Geehan, Douglas M; Elsey, James K; Sutton, Beth H; McAndrew, Mark P; Gross, Ronald I; Reed, Donald N; Van Boerum, Don H; Esposito, Thomas J; Albrecht, Roxie M; Sarani, Babak; Shapiro, David S; Wiggins-Dohlvik, Katie; Stewart, Ronald M

2017-05-01

In the United States, there is a perceived divide regarding the benefits and risks of firearm ownership. The American College of Surgeons Committee on Trauma Injury Prevention and Control Committee designed a survey to evaluate Committee on Trauma (COT) member attitudes about firearm ownership, freedom, responsibility, physician-patient freedom and policy, with the objective of using survey results to inform firearm injury prevention policy development. A 32-question survey was sent to 254 current U.S. COT members by email using Qualtrics. SPSS was used for χ exact tests and nonparametric tests, with statistical significance being less than 0.05. Our response rate was 93%, 43% of COT members have firearm(s) in their home, 88% believe that the American College of Surgeons should give the highest or a high priority to reducing firearm-related injuries, 86% believe health care professionals should be allowed to counsel patients on firearms safety, 94% support federal funding for firearms injury prevention research. The COT participants were asked to provide their opinion on the American College of Surgeons initiating advocacy efforts and there was 90% or greater agreement on 7 of 15 and 80% or greater on 10 of 15 initiatives. The COT surgeons agree on: (1) the importance of formally addressing firearm injury prevention, (2) allowing federal funds to support research on firearms injury prevention, (3) retaining the ability of health care professionals to counsel patients on firearms-related injury prevention, and (4) the majority of policy initiatives targeted to reduce interpersonal violence and firearm injury. It is incumbent on trauma and injury prevention organizations to leverage these consensus-based results to initiate prevention, advocacy, and other efforts to decrease firearms injury and death. Prognostic/epidemiologic study, level I; therapeutic care, level II.

48 CFR 52.225-2 - Buy American Act Certificate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... listed in paragraph (b) of this provision, is a domestic end product and that for other than COTS items... COTS item and does not meet the component test in paragraph (2) of the definition of “domestic end product.” The terms “commercially available off-the-shelf (COTS) item, ” “component,” “domestic end...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Noble, Stephen R.; Hudson, James G.

Here, vertical sounding measurements within stratocumuli during two aircraft field campaigns, Marine Stratus/stratocumulus Experiment (MASE) and Physics of Stratocumulus Top (POST), are used to validate Moderate Resolution Imaging Spectroradiometer (MODIS) cloud optical thickness (COT), cloud liquid water path (LWP), and cloud effective radius (r e). In situ COT, LWP, and r e were calculated using 5 m vertically averaged droplet probe measurements of complete vertical cloud penetrations. MODIS COT, LWP, and r e 1 km pixels were averaged along these penetrations. COT comparisons in POST showed strong correlations and a near 1:1 relationship. In MASE, comparisons showed strong correlations; however,more » MODIS COT exceeded in situ COT, likely due to larger temporal differences between MODIS and in situ measurements. LWP comparisons between two cloud probes show good agreement for POST but not MASE, giving confidence to POST data. Both projects provided strong LWP correlations but MODIS exceeded in situ by 14–36%. MODIS in situ r e correlations were strong, but MODIS 2.1 µm r e exceeded in situ r e, which contributed to LWP bias; in POST, MODIS r e was 20–30% greater than in situ r e. Maximum in situ r e near cloud top showed comparisons nearer 1:1. Other MODIS r e bands (3.7 µm and 1.6 µm) showed similar comparisons. Temporal differences between MODIS and in situ measurements, airplane speed differences, and cloud probe artifacts were likely causes of weaker MASE correlations. POST COT comparison was best for temporal differences under 20 min. POST data validate MODIS COT but it also implies a positive MODIS r e bias that propagates to LWP while still capturing variability.« less

Advance care planning: the impact of Ceiling of Treatment plans in patients with Coordinate My Care.

PubMed

Broadhurst, Helen Lucy; Droney, Joanne; Callender, Tom; Shaw, Amanda; Riley, Julia

2018-03-22

The aim of this evaluation is to describe the components and results of urgent care planning in Coordinate My Care (CMC), a digital clinical service for patients with life-limiting illness, for use if a patient is unable to make or express choices. Ceiling of treatment (CoT) plans were created detailing where the patient would like to receive their care and how aggressive medical interventions should be. A retrospective service evaluation was completed of all CMC records created between December 2015 and September 2016 (n=6854). CMC records were divided into two cohorts: those with a CoT plan and those without. The factors associated with these cohorts were reviewed including age, diagnosis, resuscitation status and preferences for place of death (PPD). Analysis of the non-mandatory free text section was carried out. Two-thirds of patients had recorded decisions about CoT. Regardless of which CoT option was chosen, for most patients, PPD was home or care home. Patients with a CoT plan were more likely to have a documented resuscitation status.Patients with a CoT were more likely to die in their PPD (82%vs71%, OR 1.79, p<0.0001). A higher proportion of patients with a CoT decision died outside hospital. This analysis demonstrates that a substantial proportion of patients are willing to engage in urgent care planning. Three facets of urgent care planning identified include PPD, CoT and resuscitation status. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

MODIS comparisons with northeastern Pacific in situ stratocumulus microphysics

PubMed Central

Noble, Stephen R.

2015-01-01

Abstract Vertical sounding measurements within stratocumuli during two aircraft field campaigns, Marine Stratus/stratocumulus Experiment (MASE) and Physics of Stratocumulus Top (POST), are used to validate Moderate Resolution Imaging Spectroradiometer (MODIS) cloud optical thickness (COT), cloud liquid water path (LWP), and cloud effective radius (r e). In situ COT, LWP, and r e were calculated using 5 m vertically averaged droplet probe measurements of complete vertical cloud penetrations. MODIS COT, LWP, and r e 1 km pixels were averaged along these penetrations. COT comparisons in POST showed strong correlations and a near 1:1 relationship. In MASE, comparisons showed strong correlations; however, MODIS COT exceeded in situ COT, likely due to larger temporal differences between MODIS and in situ measurements. LWP comparisons between two cloud probes show good agreement for POST but not MASE, giving confidence to POST data. Both projects provided strong LWP correlations but MODIS exceeded in situ by 14–36%. MODIS in situ r e correlations were strong, but MODIS 2.1 µm r e exceeded in situ r e, which contributed to LWP bias; in POST, MODIS r e was 20–30% greater than in situ r e. Maximum in situ r e near cloud top showed comparisons nearer 1:1. Other MODIS r e bands (3.7 µm and 1.6 µm) showed similar comparisons. Temporal differences between MODIS and in situ measurements, airplane speed differences, and cloud probe artifacts were likely causes of weaker MASE correlations. POST COT comparison was best for temporal differences under 20 min. POST data validate MODIS COT but it also implies a positive MODIS r e bias that propagates to LWP while still capturing variability. PMID:27708990

MODIS comparisons with northeastern Pacific in situ stratocumulus microphysics

DOE PAGES

Noble, Stephen R.; Hudson, James G.

2015-07-22

Here, vertical sounding measurements within stratocumuli during two aircraft field campaigns, Marine Stratus/stratocumulus Experiment (MASE) and Physics of Stratocumulus Top (POST), are used to validate Moderate Resolution Imaging Spectroradiometer (MODIS) cloud optical thickness (COT), cloud liquid water path (LWP), and cloud effective radius (r e). In situ COT, LWP, and r e were calculated using 5 m vertically averaged droplet probe measurements of complete vertical cloud penetrations. MODIS COT, LWP, and r e 1 km pixels were averaged along these penetrations. COT comparisons in POST showed strong correlations and a near 1:1 relationship. In MASE, comparisons showed strong correlations; however,more » MODIS COT exceeded in situ COT, likely due to larger temporal differences between MODIS and in situ measurements. LWP comparisons between two cloud probes show good agreement for POST but not MASE, giving confidence to POST data. Both projects provided strong LWP correlations but MODIS exceeded in situ by 14–36%. MODIS in situ r e correlations were strong, but MODIS 2.1 µm r e exceeded in situ r e, which contributed to LWP bias; in POST, MODIS r e was 20–30% greater than in situ r e. Maximum in situ r e near cloud top showed comparisons nearer 1:1. Other MODIS r e bands (3.7 µm and 1.6 µm) showed similar comparisons. Temporal differences between MODIS and in situ measurements, airplane speed differences, and cloud probe artifacts were likely causes of weaker MASE correlations. POST COT comparison was best for temporal differences under 20 min. POST data validate MODIS COT but it also implies a positive MODIS r e bias that propagates to LWP while still capturing variability.« less

Chronic Pain, Chronic Opioid Addiction: a Complex Nexus.

PubMed

Salsitz, Edwin A

2016-03-01

Over the past two decades, there has been a significant increase in the prescribing of opioids, with associated increases in opioid addiction and overdose deaths. This article reviews the evidence for the effectiveness and risk of developing an opioid use disorder (OUD) in those patients treated with chronic opioid therapy (COT) for chronic non-cancer pain (CNCP). Rates of development of OUD range from 0-50 %, and aberrant drug related behaviors (ADRBs) are reported to be 20 %. Health care providers must properly assess, screen, and carefully monitor patients on COT utilizing evidence-based tools.

Estimating vertical profiles of water-cloud droplet effective radius from SWIR satellite measurements via a statistical model derived from CloudSat observations

NASA Astrophysics Data System (ADS)

Nagao, T. M.; Murakami, H.; Nakajima, T. Y.

2017-12-01

This study proposes an algorithm to estimate vertical profiles of cloud droplet effective radius (CDER-VP) for water clouds from shortwave infrared (SWIR) measurements of Himawari-8/AHI via a statistical model of CDER-VP derived from CloudSat observation. Several similar algorithms in previous studies utilize a spectral radiance matching on the assumption of simultaneous observations of CloudSat and Aqua/MODIS. However, our algorithm does not assume simultaneous observations with CloudSat. First, in advance, a database (DB) of CDER-VP is prepared by the following procedure: TOA radiances at 0.65, 2.3 and 10.4-μm bands of the AHI are simulated using CDER-VP and cloud optical depth vertical profile (COD-VP) contained in the CloudSat 2B-CWC-RVOD and 2B-TAU products. Cloud optical thickness (COT), Column-CDER and cloud top height (CTH) are retrieved from the simulated radiances using a traditional retrieval algorithm with vertically homogeneous cloud model (1-SWIR VHC method). The CDER-VP is added to the DB by using the COT and Column-CDER retrievals as a key of the DB. Then by using principal component (PC) analysis, up to three PC vectors of the CDER-VPs in the DB are extracted. Next, the algorithm retrieves CDER-VP from actual AHI measurements by the following procedure: First, COT, Column-CDER and CTH are retrieved from TOA radiances at 0.65, 2.3 and 10.4-μm bands of the AHI using by 1-SWIR VHC method. Then, the PC vectors of CDER-VP is fetched from the DB using the COT and Column-CDER retrievals as the key of the DB. Finally, using coefficients of the PC vectors of CDER-VP as variables for retrieval, CDER-VP, COT and CTH are retrieved from TOA radiances at 0.65, 1.6, 2.3, 3.9 and 10.4-μm bands of the AHI based on optimal estimation method with iterative radiative transfer calculation. The simulation result showed the CDER-VP retrieval errors were almost smaller than 3 - 4 μm. The CDER retrieval errors at the cloud base were almost larger than the others (e.g. CDER at cloud top), especially when COT and CDER was large. The tendency can be explained by less sensitivities of SWIRs to CDER at cloud base. Additionally, as a case study, this study will attempt to apply the algorithm to the AHI's high-frequency observations, and to interpret the time series of the CDER-VP retrievals in terms of temporal evolution of water clouds.

Application of Fault-Tolerant Computing For Spacecraft Using Commercial-Off-The-Shelf Microprocessors

DTIC Science & Technology

2000-06-01

real - time operating system and design of a human-computer interface (HCI) for a triple modular redundant (TMR) fault-tolerant microprocessor for use in space-based applications. Once disadvantage of using COTS hardware components is their susceptibility to the radiation effects present in the space environment. and specifically, radiation-induced single-event upsets (SEUs). In the event of an SEU, a fault-tolerant system can mitigate the effects of the upset and continue to process from the last known correct system state. The TMR basic hardware

Estimating Cloud optical thickness from SEVIRI, for air quality research, by implementing a semi-analytical cloud retrieval algorithm

NASA Astrophysics Data System (ADS)

Pandey, Praveen; De Ridder, Koen; van Looy, Stijn; van Lipzig, Nicole

2010-05-01

Clouds play an important role in Earth's climate system. As they affect radiation hence photolysis rate coefficients (ozone formation),they also affect the air quality at the surface of the earth. Thus, a satellite remote sensing technique is used to retrieve the cloud properties for air quality research. The geostationary satellite, Meteosat Second Generation (MSG) has onboard, the Spinning Enhanced Visible and Infrared Imager (SEVIRI). The channels in the wavelength 0.6 µm and 1.64 µm are used to retrieve cloud optical thickness (COT). The study domain is over Europe covering a region between 35°N-70°N and 5°W-30°E, centred over Belgium. The steps involved in pre-processing the EUMETSAT level 1.5 images are described, which includes, acquisition of digital count number, radiometric conversion using offsets and slopes, estimation of radiance and calculation of reflectance. The Sun-earth-satellite geometry also plays an important role. A semi-analytical cloud retrieval algorithm (Kokhanovsky et al., 2003) is implemented for the estimation of COT. This approach doesn't involve the conventional look-up table approach, hence it makes the retrieval independent of numerical radiative transfer solutions. The semi-analytical algorithm is implemented on a monthly dataset of SEVIRI level 1.5 images. Minimum reflectance in the visible channel, at each pixel, during the month is accounted as the surface albedo of the pixel. Thus, monthly variation of COT over the study domain is prepared. The result so obtained, is compared with the COT products of Satellite Application Facility on Climate Monitoring (CM SAF). Henceforth, an approach to assimilate the COT for air quality research is presented. Address of corresponding author: Praveen Pandey, VITO- Flemish Institute for Technological Research, Boeretang 200, B 2400, Mol, Belgium E-mail: praveen.pandey@vito.be

NASA Crew Personal Active Dosimeters (CPADs): Leveraging Novel Terrestrial Personal Radiation Monitoring Capabilities for Space Exploration

NASA Technical Reports Server (NTRS)

Leitgab, Martin; Semones, Edward; Lee, Kerry

2016-01-01

The NASA Space Radiation Analysis Group (SRAG) is developing novel Crew Personal Active Dosimeters (CAPDs) for upcoming crewed space exploration missions and beyond. To reduce the resource footprint of the project a COTS dosimeter base is used for the development of CPADs. This base was identified from evaluations of existing COTS personal dosimeters against the concept of operations of future crewed missions and tests against detection requirements for radiation characteristic of the space environment. CPADs exploit operations efficiencies from novel features for space flight personal dosimeters such as real-time dose feedback, and autonomous measuring and data transmission capabilities. Preliminary CPAD design, results of radiation testing and aspects of operational integration will be presented.

Hardness Assurance Techniques for New Generation COTS Devices

NASA Technical Reports Server (NTRS)

Lee, C. I.; Rax, B. G.; Johnston, A. H.

1996-01-01

Hardness Assurance (HA) techniques and total dose radiation characterization data for new generation linear and COTS devices from various manufacturers are presented. A bipolar op amp showed significant degradation at HDR, not at low dose rate environment. New generation low-power op amps showed more degradation at low voltage applications. HA test techniques for COTS devices are presented in this paper.

Implementation of COTs Hardware in Non-Critical Space Applications: A Brief Tutorial

NASA Technical Reports Server (NTRS)

Yoder, Geoffrey L.

2004-01-01

Approaches used for manned applications include limited items such as CD-players evaluated for safety to high criticality applications where the COTs hardware is evaluated on a case-by-case basis for the application and commensurate screening and qualification testing. COTS hardware is successfully implemented in both the International Space Station and Space Shuttle but requires evaluation and modifications for the application. Screening and qualification of COTs hardware used in critical applications may need to be more extensive and stringent than traditional military screening. Evaluation for: a) Suitability for the application; b) Safety; c) Reliability and maintainability; and d) Workmanship.

Within-subject variation of the salivary 3HC/COT ratio in regular daily smokers: prospects for estimating CYP2A6 enzyme activity in large-scale surveys of nicotine metabolic rate.

PubMed

Lea, Rod A; Dickson, Stuart; Benowitz, Neal L

2006-01-01

Nicotine is the major addictive compound in tobacco and is responsible for tobacco dependence. It is primarily metabolized to cotinine (COT) and trans-3'-hydroxycotinine (3HC) by the liver enzyme cytochrome P-450 2A6 (CYP2A6). The 3HC/COT ratio measured in the saliva of smokers is highly correlated with the intrinsic hepatic clearance of nicotine and, therefore, may be a useful non-invasive marker of CYP2A6 activity and metabolic rate of nicotine. This study assessed within-subject variation in salivary 3HC/COT ratios in six regular daily smokers. Our data provide evidence that 1. variation in the 3HC/COT ratio is not dependent on the time of sampling during the day (i.e., morning vs. night ) (P > 0.1) and 2. the average within-subject biological variation in the 3HC/COT ratio is approximately 26%. These findings should be useful for designing large-scale population surveys to assess the variation in the metabolic rate of nicotine (via CYP2A6) in smokers.

LPS-induced chemokine expression in both MyD88-dependent and -independent manners is regulated by Cot/Tpl2-ERK axis in macrophages.

PubMed

Bandow, Kenjiro; Kusuyama, Joji; Shamoto, Mitsuo; Kakimoto, Kyoko; Ohnishi, Tomokazu; Matsuguchi, Tetsuya

2012-05-21

LPS signaling is mediated through MyD88-dependent and -independent pathways, activating NF-?B, MAP kinases and IRF3. Cot/Tpl2 is an essential upstream kinase in LPS-mediated activation of ERKs. Here we explore the roles of MyD88 and Cot/Tpl2 in LPS-induced chemokine expression by studying myd88(-/-) and cot/tpl2(-/-) macrophages. Among the nine LPS-responsive chemokines examined, mRNA induction of ccl5, cxcl10, and cxcl13 is mediated through the MyD88-independent pathway. Notably, Cot/Tpl2-ERK signaling axis exerts negative effects on the expression of these three chemokines. In contrast, LPS-induced gene expression of ccl2, ccl7, cxcl2, cxcl3, ccl8, and cxcl9 is mediated in the MyD88-dependent manner. The Cot/Tpl2-ERK axis promotes the expression of the first four and inhibits the expression of the latter two. Thus, LPS induces expression of multiple chemokines through various signaling pathways in macrophages. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Real-time FPGA-based radar imaging for smart mobility systems

NASA Astrophysics Data System (ADS)

Saponara, Sergio; Neri, Bruno

2016-04-01

The paper presents an X-band FMCW (Frequency Modulated Continuous Wave) Radar Imaging system, called X-FRI, for surveillance in smart mobility applications. X-FRI allows for detecting the presence of targets (e.g. obstacles in a railway crossing or urban road crossing, or ships in a small harbor), as well as their speed and their position. With respect to alternative solutions based on LIDAR or camera systems, X-FRI operates in real-time also in bad lighting and weather conditions, night and day. The radio-frequency transceiver is realized through COTS (Commercial Off The Shelf) components on a single-board. An FPGA-based baseband platform allows for real-time Radar image processing.

A study of applications scribe frame data verifications using design rule check

NASA Astrophysics Data System (ADS)

Saito, Shoko; Miyazaki, Masaru; Sakurai, Mitsuo; Itoh, Takahisa; Doi, Kazumasa; Sakurai, Norioko; Okada, Tomoyuki

2013-06-01

In semiconductor manufacturing, scribe frame data generally is generated for each LSI product according to its specific process design. Scribe frame data is designed based on definition tables of scanner alignment, wafer inspection and customers specified marks. We check that scribe frame design is conforming to specification of alignment and inspection marks at the end. Recently, in COT (customer owned tooling) business or new technology development, there is no effective verification method for the scribe frame data, and we take a lot of time to work on verification. Therefore, we tried to establish new verification method of scribe frame data by applying pattern matching and DRC (Design Rule Check) which is used in device verification. We would like to show scheme of the scribe frame data verification using DRC which we tried to apply. First, verification rules are created based on specifications of scanner, inspection and others, and a mark library is also created for pattern matching. Next, DRC verification is performed to scribe frame data. Then the DRC verification includes pattern matching using mark library. As a result, our experiments demonstrated that by use of pattern matching and DRC verification our new method can yield speed improvements of more than 12 percent compared to the conventional mark checks by visual inspection and the inspection time can be reduced to less than 5 percent if multi-CPU processing is used. Our method delivers both short processing time and excellent accuracy when checking many marks. It is easy to maintain and provides an easy way for COT customers to use original marks. We believe that our new DRC verification method for scribe frame data is indispensable and mutually beneficial.

Budget impact model in moderate-to-severe psoriasis vulgaris assessing effects of calcipotriene and betamethasone dipropionate foam on per-patient standard of care costs.

PubMed

Asche, Carl V; Kim, Minchul; Feldman, Steven R; Zografos, Panagiotis; Lu, Minyi

2017-09-01

To develop a budget impact model (BIM) for estimating the financial impact of formulary adoption and uptake of calcipotriene and betamethasone dipropionate (C/BD) foam (0.005%/0.064%) on the costs of biologics for treating moderate-to-severe psoriasis vulgaris in a hypothetical US healthcare plan with 1 million members. This BIM incorporated epidemiologic data, market uptake assumptions, and drug utilization costs, simulating the treatment mix for patients who are candidates for biologics before (Scenario #1) and after (Scenario #2) the introduction of C/BD foam. Predicted outcomes were expressed in terms of the annual cost of treatment (COT) and the COT per member per month (PMPM). At year 1, C/BD foam had the lowest per-patient cost ($9,913) necessary to achieve a Psoriasis Area and Severity Index (PASI)-75 response compared with etanercept ($73,773), adalimumab ($92,871), infliximab ($34,048), ustekinumab ($83,975), secukinumab ($113,858), apremilast ($47,960), and ixekizumab ($62,707). Following addition of C/BD foam to the formulary, the annual COT for moderate-to-severe psoriasis would decrease by $36,112,572 (17.91%, from $201,621,219 to $165,508,647). The COT PMPM is expected to decrease by $3.00 (17.86%, from $16.80 to $13.80). Drug costs were based on Medi-Span reference pricing (January 21, 2016); differences in treatment costs for drug administration, laboratory monitoring, or adverse events were not accounted for. Potentially confounding were the definition of "moderate-to-severe" and the heterogeneous efficacy data. The per-patient cost for PASI-75 response at year 1 was estimated from short-term efficacy data for C/BD foam and apremilast only. The introduction of C/BD foam is expected to decrease the annual COT for moderate-to-severe psoriasis treatable with biologics by $36,112,572 for a hypothetical US healthcare plan with 1 million plan members, and to lower the COT PMPM by $3.00.

Development of COTS ADC SEE Test System for the ATLAS LArCalorimeter Upgrade

DOE PAGES

Hu, Xue -Ye; Chen, Hu -Cheng; Chen, Kai; ...

2014-12-01

Radiation-tolerant, high speed, high density and low power commercial off-the-shelf (COTS) analog-to-digital converters (ADCs) are planned to be used in the upgrade to the Liquid Argon (LAr) calorimeter front end (FE) trigger readout electronics. Total ionization dose (TID) and single event effect (SEE) are two important radiation effects which need to be characterized on COTS ADCs. In our initial TID test, Texas Instruments (TI) ADS5272 was identified to be the top performer after screening a total 17 COTS ADCs from different manufacturers with dynamic range and sampling rate meeting the requirements of the FE electronics. Another interesting feature of ADS5272more » is its 6.5 clock cycles latency, which is the shortest among the 17 candidates. Based on the TID performance, we have designed a SEE evaluation system for ADS5272, which allows us to further assess its radiation tolerance. In this paper, we present a detailed design of ADS5272 SEE evaluation system and show the effectiveness of this system while evaluating ADS5272 SEE characteristics in multiple irradiation tests. According to TID and SEE test results, ADS5272 was chosen to be implemented in the full-size LAr Trigger Digitizer Board (LTDB) demonstrator, which will be installed on ATLAS calorimeter during the 2014 Long Shutdown 1 (LS1).« less

NICOTINE METABOLISM IN PREGNANT AND NON-PREGNANT RABBITS

PubMed Central

Tutka, Piotr; Dempsey, Delia A.; Jacob, Peyton; Benowitz, Neal L.; Kroetz, Deanna L.

2010-01-01

Smoking remains a major public health concern during pregnancy and is associated with numerous adverse effects. Recently the clearance of nicotine (NIC) and cotinine (COT) was shown to be substantially increased in pregnant women compared to non-pregnant controls. The present study investigated the usefulness of the rabbit for studying the molecular basis for the observed changes in NIC and COT disposition during pregnancy. NIC was largely metabolized to COT in rabbit liver microsomes (approximately 50% of total metabolism) with significant amounts of nicotine-N’-oxide and nornicotine also being detected. The conversion of NIC to COT was also detected in rabbit placental and fetal liver microsomes albeit at only a fraction of the rate in adult rabbit liver microsomes. The major products of COT metabolism in rabbit liver microsomes were 5’-hydroxycotinine, cotinine-N’-oxide and norcotinine. Differences between human and rabbit liver were most apparent for COT, with the major human metabolite 3’-hydroxycotinine, being formed at only low levels in rabbit liver microsomes. Pregnancy had no effect on the metabolism of NIC or on the expression of CYP2A6 immunoreactive proteins in rabbit liver microsomes. These studies provide a complete quantitative assessment of NIC metabolism in rabbit liver microsomes and suggest that the rabbit may not be an appropriate animal model to study the effects of pregnancy on NIC and COT metabolism. However, a molecular understanding of these effects is essential for prediction of the pharmacological and toxicological consequences of smoking during pregnancy. PMID:18686186

Cot-nursing versus incubator care for preterm infants.

PubMed

Gray, Peter H; Flenady, Vicki

2011-08-10

Preterm infants are usually nursed in incubators, but cot-nursing may provide an alternative. While there may be benefits of nursing preterm infants in open cots, there may be potential risks such as nosocomial infection caused by more handling due to easier access. To assess effects of cot-nursing versus incubator care on temperature control and weight gain in preterm infants. The standard search strategy of the Cochrane Neonatal Review Group was used. This included searches of electronic databases including the Cochrane Central Register of Controlled Trials (The Cochrane Library), Oxford Database of Perinatal Trials, MEDLINE, CINAHL, and EMBASE, as well as previous reviews including cross references through November 2009. All trials using random or quasi-random patient allocation in which infants receiving care in standard newborn cots were compared to infants managed in a conventional air heated incubator. The authors independently assessed trial quality and extracted data for the primary outcomes of temperature control and weight gain. Meta-analysis was conducted using a fixed-effect model. Eleven potential studies were identified of which five, involving 247 infants, were included in this review. When compared to incubator care, cot-nursing resulted in no significant difference in mean body temperature (MD 0.02 degrees C; 95% CI -0.02 to 0.07, four trials), though the one trial that reported on episodes of hyperthermia found this to be statistically more common in the cot-nursing group (RR 1.48; 95% CI 1.04 to 2.09). There were no statistically significant differences in weight gain. In the cot-nursing group, fewer infants were breast fed on discharge (typical RR 0.74; 95% CI 0.48 to 1.14, three trials, 150 infants) and fewer infants died prior to hospital discharge (typical RR 0.59, 95% CI 0.28 to 1.25, four trials, 235 infants) but these results failed to reach statistical significance. The comparison of cot-nursing using a heated water-filled mattress versus incubator care, which included five trials and a total of 231 infants, produced similar results. Cot-nursing with warming of the nursery resulted in statistically significantly smaller weight gain during week one compared to the incubator group in one trial that involved 38 infants (MD -5.90 g/kg/day; 95% CI -11.13 to -0.67) but no significant difference was found for weeks two and three. Cot-nursing using a heated water-filled mattress has similar effects to incubator care with regard to temperature control and weight gain. Important clinical outcomes need to be investigated further using randomised controlled trials. This is especially the case in the situation of developing countries, where differences in these outcomes are likely to be encountered. As limited data is available on cot-nursing using a space-heated room, this method is not recommended as practice.

Internet Based Remote Operations

NASA Technical Reports Server (NTRS)

Chamberlain, James

1999-01-01

This is the Final Report for the Internet Based Remote Operations Contract, has performed payload operations research support tasks March 1999 through September 1999. These tasks support the GSD goal of developing a secure, inexpensive data, voice, and video mission communications capability between remote payload investigators and the NASA payload operations team in the International Space Station (ISS) era. AZTek has provided feedback from the NASA payload community by utilizing its extensive payload development and operations experience to test and evaluate remote payload operations systems. AZTek has focused on use of the "public Internet" and inexpensive, Commercial-off-the-shelf (COTS) Internet-based tools that would most benefit "small" (e.g., $2 Million or less) payloads and small developers without permanent remote operations facilities. Such projects have limited budgets to support installation and development of high-speed dedicated communications links and high-end, custom ground support equipment and software. The primary conclusions of the study are as follows: (1) The trend of using Internet technology for "live" collaborative applications such as telescience will continue. The GSD-developed data and voice capabilities continued to work well over the "public" Internet during this period. 2. Transmitting multiple voice streams from a voice-conferencing server to a client PC to be mixed and played on the PC is feasible. 3. There are two classes of voice vendors in the market: - Large traditional phone equipment vendors pursuing integration of PSTN with Internet, and Small Internet startups.The key to selecting a vendor will be to find a company sufficiently large and established to provide a base voice-conferencing software product line for the next several years.

NASA Operational Simulator for Small Satellites: Tools for Software Based Validation and Verification of Small Satellites

NASA Technical Reports Server (NTRS)

Grubb, Matt

2016-01-01

The NASA Operational Simulator for Small Satellites (NOS3) is a suite of tools to aid in areas such as software development, integration test (IT), mission operations training, verification and validation (VV), and software systems check-out. NOS3 provides a software development environment, a multi-target build system, an operator interface-ground station, dynamics and environment simulations, and software-based hardware models. NOS3 enables the development of flight software (FSW) early in the project life cycle, when access to hardware is typically not available. For small satellites there are extensive lead times on many of the commercial-off-the-shelf (COTS) components as well as limited funding for engineering test units (ETU). Considering the difficulty of providing a hardware test-bed to each developer tester, hardware models are modeled based upon characteristic data or manufacturers data sheets for each individual component. The fidelity of each hardware models is such that FSW executes unaware that physical hardware is not present. This allows binaries to be compiled for both the simulation environment, and the flight computer, without changing the FSW source code. For hardware models that provide data dependent on the environment, such as a GPS receiver or magnetometer, an open-source tool from NASA GSFC (42 Spacecraft Simulation) is used to provide the necessary data. The underlying infrastructure used to transfer messages between FSW and the hardware models can also be used to monitor, intercept, and inject messages, which has proven to be beneficial for VV of larger missions such as James Webb Space Telescope (JWST). As hardware is procured, drivers can be added to the environment to enable hardware-in-the-loop (HWIL) testing. When strict time synchronization is not vital, any number of combinations of hardware components and software-based models can be tested. The open-source operator interface used in NOS3 is COSMOS from Ball Aerospace. For testing, plug-ins are implemented in COSMOS to control the NOS3 simulations, while the command and telemetry tools available in COSMOS are used to communicate with FSW. NOS3 is actively being used for FSW development and component testing of the Simulation-to-Flight 1 (STF-1) CubeSat. As NOS3 matures, hardware models have been added for common CubeSat components such as Novatel GPS receivers, ClydeSpace electrical power systems and batteries, ISISpace antenna systems, etc. In the future, NASA IVV plans to distribute NOS3 to other CubeSat developers and release the suite to the open-source community.

Two-panel LCOS-based projection system: a potentially compact high-resolution avionics display

NASA Astrophysics Data System (ADS)

Sharp, Gary D.; Chen, Jianmin; Robinson, Michael B.; Korah, John K.

2003-09-01

Military displays have been limited first by the availability of CRT and then AMLCD for color multifunctional displays. Projection display technology has been offered as an alternative. With the growth of the LCOS based consumer projection display industry, commercially off the shelf (COTS) components and technology are becoming readily available. A projection display system addresses the lessons learned from the CRT or AMLCD based attempts. This approach presents multiple vendors and user defined aspect ratio, resolution, brightness and color. This paper will present the latest work at ColorLink, Inc. on a two-panel LCOS based projection light engine developed for the consumer industry driven Rear Projection Television (RPTV) market. This engine demonstrates throughput, contrast and color performance that exceeds military requirements using COTS technology and components. We will introduce the core technology and philosophy followed by this industry in defining such a product.

Data Service Provider Cost Estimation Tool

NASA Technical Reports Server (NTRS)

Fontaine, Kathy; Hunolt, Greg; Booth, Arthur L.; Banks, Mel

2011-01-01

The Data Service Provider Cost Estimation Tool (CET) and Comparables Database (CDB) package provides to NASA s Earth Science Enterprise (ESE) the ability to estimate the full range of year-by-year lifecycle cost estimates for the implementation and operation of data service providers required by ESE to support its science and applications programs. The CET can make estimates dealing with staffing costs, supplies, facility costs, network services, hardware and maintenance, commercial off-the-shelf (COTS) software licenses, software development and sustaining engineering, and the changes in costs that result from changes in workload. Data Service Providers may be stand-alone or embedded in flight projects, field campaigns, research or applications projects, or other activities. The CET and CDB package employs a cost-estimation-by-analogy approach. It is based on a new, general data service provider reference model that provides a framework for construction of a database by describing existing data service providers that are analogs (or comparables) to planned, new ESE data service providers. The CET implements the staff effort and cost estimation algorithms that access the CDB and generates the lifecycle cost estimate for a new data services provider. This data creates a common basis for an ESE proposal evaluator for considering projected data service provider costs.

A Real-Time Telemetry Simulator of the IUS Spacecraft

NASA Technical Reports Server (NTRS)

Drews, Michael E.; Forman, Douglas A.; Baker, Damon M.; Khazoyan, Louis B.; Viazzo, Danilo

1998-01-01

A real-time telemetry simulator of the IUS spacecraft has recently entered operation to train Flight Control Teams for the launch of the AXAF telescope from the Shuttle. The simulator has proven to be a successful higher fidelity implementation of its predecessor, while affirming the rapid development methodology used in its design. Although composed of COTS hardware and software, the system simulates the full breadth of the mission: Launch, Pre-Deployment-Checkout, Burn Sequence, and AXAF/IUS separation. Realism is increased through patching the system into the operations facility to simulate IUS telemetry, Shuttle telemetry, and the Tracking Station link (commands and status message).

Retrieval of radiative and microphysical properties of clouds from multispectral infrared measurements

NASA Astrophysics Data System (ADS)

Iwabuchi, Hironobu; Saito, Masanori; Tokoro, Yuka; Putri, Nurfiena Sagita; Sekiguchi, Miho

2016-12-01

Satellite remote sensing of the macroscopic, microphysical, and optical properties of clouds are useful for studying spatial and temporal variations of clouds at various scales and constraining cloud physical processes in climate and weather prediction models. Instead of using separate independent algorithms for different cloud properties, a unified, optimal estimation-based cloud retrieval algorithm is developed and applied to moderate resolution imaging spectroradiometer (MODIS) observations using ten thermal infrared bands. The model considers sensor configurations, background surface and atmospheric profile, and microphysical and optical models of ice and liquid cloud particles and radiative transfer in a plane-parallel, multilayered atmosphere. Measurement and model errors are thoroughly quantified from direct comparisons of clear-sky observations over the ocean with model calculations. Performance tests by retrieval simulations show that ice cloud properties are retrieved with high accuracy when cloud optical thickness (COT) is between 0.1 and 10. Cloud-top pressure is inferred with uncertainty lower than 10 % when COT is larger than 0.3. Applying the method to a tropical cloud system and comparing the results with the MODIS Collection 6 cloud product shows good agreement for ice cloud optical thickness when COT is less than about 5. Cloud-top height agrees well with estimates obtained by the CO2 slicing method used in the MODIS product. The present algorithm can detect optically thin parts at the edges of high clouds well in comparison with the MODIS product, in which these parts are recognized as low clouds by the infrared window method. The cloud thermodynamic phase in the present algorithm is constrained by cloud-top temperature, which tends not to produce results with an ice cloud that is too warm and liquid cloud that is too cold.

Real-time synchronized multiple-sensor IR/EO scene generation utilizing the SGI Onyx2

NASA Astrophysics Data System (ADS)

Makar, Robert J.; O'Toole, Brian E.

1998-07-01

An approach to utilize the symmetric multiprocessing environment of the Silicon Graphics Inc.R (SGI) Onyx2TM has been developed to support the generation of IR/EO scenes in real-time. This development, supported by the Naval Air Warfare Center Aircraft Division (NAWC/AD), focuses on high frame rate hardware-in-the-loop testing of multiple sensor avionics systems. In the past, real-time IR/EO scene generators have been developed as custom architectures that were often expensive and difficult to maintain. Previous COTS scene generation systems, designed and optimized for visual simulation, could not be adapted for accurate IR/EO sensor stimulation. The new Onyx2 connection mesh architecture made it possible to develop a more economical system while maintaining the fidelity needed to stimulate actual sensors. An SGI based Real-time IR/EO Scene Simulator (RISS) system was developed to utilize the Onyx2's fast multiprocessing hardware to perform real-time IR/EO scene radiance calculations. During real-time scene simulation, the multiprocessors are used to update polygon vertex locations and compute radiometrically accurate floating point radiance values. The output of this process can be utilized to drive a variety of scene rendering engines. Recent advancements in COTS graphics systems, such as the Silicon Graphics InfiniteRealityR make a total COTS solution possible for some classes of sensors. This paper will discuss the critical technologies that apply to infrared scene generation and hardware-in-the-loop testing using SGI compatible hardware. Specifically, the application of RISS high-fidelity real-time radiance algorithms on the SGI Onyx2's multiprocessing hardware will be discussed. Also, issues relating to external real-time control of multiple synchronized scene generation channels will be addressed.

The Knowledge-Based Software Assistant: Beyond CASE

NASA Technical Reports Server (NTRS)

Carozzoni, Joseph A.

1993-01-01

This paper will outline the similarities and differences between two paradigms of software development. Both support the whole software life cycle and provide automation for most of the software development process, but have different approaches. The CASE approach is based on a set of tools linked by a central data repository. This tool-based approach is data driven and views software development as a series of sequential steps, each resulting in a product. The Knowledge-Based Software Assistant (KBSA) approach, a radical departure from existing software development practices, is knowledge driven and centers around a formalized software development process. KBSA views software development as an incremental, iterative, and evolutionary process with development occurring at the specification level.

Preparation of AN Electrode Modified with a Thermostable Enzyme BACILLUS Subtilis COTA by Electrodeposition

NASA Astrophysics Data System (ADS)

Watanabe, Toshio; Yamada, Yohei; Motonaka, Junko; Yabutani, Tomoki; Sakuraba, Haruhiko; Yasuzawa, Mikito

In this study, electrodeposition of thermostable enzyme Bacillus subtilis CotA, which is a laccase and has a bilirubin oxidase (BOD) activity, was investigated. The electrodeposition was operated in a mixture of Bacillus subtilis CotA in the PBS (pH 8.0) and TritonX-100 under applying potential (1100 mV vs. Ag/AgCl for 5 min.). The current response was measured by linear sweep voltammetry technique (LSV). The thermostable enzyme Bacillus subtilis CotA electrodeposited electrode was compared with a mesophile BOD electrodeposited electrode. As a result, the Bacillus subtilis CotA modified electrode showed better sensitivity and long-term stability than the mesophile BOD modified electrode.

A Simplified Model of ARIS for Optimal Controller Design

NASA Technical Reports Server (NTRS)

Beech, Geoffrey S.; Hampton, R. David; Kross, Denny (Technical Monitor)

2001-01-01

Many space-science experiments require active vibration isolation. Boeing's Active Rack Isolation System (ARIS) isolates experiments at the rack (vs. experiment or sub-experiment) level, with multi e experiments per rack. An ARIS-isolated rack typically employs eight actuators and thirteen umbilicals; the umbilicals provide services such as power, data transmission, and cooling. Hampton, et al., used "Kane's method" to develop an analytical, nonlinear, rigid-body model of ARIS that includes full actuator dynamics (inertias). This model, less the umbilicals, was first implemented for simulation by Beech and Hampton; they developed and tested their model using two commercial-off-the-shelf (COTS) software packages. Rupert, et al., added umbilical-transmitted disturbances to this nonlinear model. Because the nonlinear model, even for the untethered system, is both exceedingly complex and "encapsulated" inside these COTS tools, it is largely inaccessible to ARIS controller designers. This paper shows that ISPR rattle-space constraints and small ARIS actuator masses permit considerable model simplification, without significant loss of fidelity. First, for various loading conditions, comparisons are made between the dynamic responses of the nonlinear model (untethered) and a truth model. Then comparisons are made among nonlinear, linearized, and linearized reduced-mass models. It is concluded that these three models all capture the significant system rigid-body dynamics, with the third being preferred due to its relative simplicity.

The continent-ocean transition at the mid-northern margin of the South China Sea

NASA Astrophysics Data System (ADS)

Gao, Jinwei; Wu, Shiguo; McIntosh, Kirk; Mi, Lijun; Yao, Bochu; Chen, Zeman; Jia, Liankai

2015-07-01

The northern margin of the South China Sea (SCS) has particular structural and stratigraphic characteristics that are somewhat different from those described in typical passive margin models. The differences are attributable to poly-phase tectonic movements and magmatic activity resulting from the interaction among the Eurasian, Philippine Sea and Indo-Australian plates. Based on several crustal-scale multi-channel seismic reflection profiles and satellite gravity data across the northern SCS margin, this paper analyzes the structures, volcanoes and deep crust of the continent-ocean transition zone (COT) at the mid-northern margin of the SCS to study the patterns and model of extension there. The results indicate that the COT is limited landward by basin-bounding faults near Baiyun sag and is bounded by seaward-dipping normal faults near the oceanic basin in our seismic lines. The shallow anatomy of the COT is characterized by rift depression, structural highs with igneous rock and/or a volcanic zone or a zone of tilted fault blocks at the distal edge. Gravity modeling revealed that a high velocity layer (HVL) with a 0.8-6-km thickness is frequently present in the slope below the lower crust. Our study shows that the HVL is only located in the eastern portion of the northern SCS margin based on the available geophysical data. We infer from this that the presence of an HVL is not required in the COT at the northern SCS margin. The magmatic intrusions and HVL may be related to partial melting caused by the decompression of a passive, upwelling asthenosphere, which resulted primarily in post-rifting underplating and magmatic emplacement or modification of the crust. Based on this study, we propose that an intermediate mode of rifting was active in the mid-northern margin of the SCS with characteristics that are closer to those of the magma-poor margins than those of volcanic margins.

Stable CoT-1 repeat RNA is abundant and associated with euchromatic interphase chromosomes

PubMed Central

Hall, Lisa L.; Carone, Dawn M.; Gomez, Alvin; Kolpa, Heather J.; Byron, Meg; Mehta, Nitish; Fackelmayer, Frank O.; Lawrence, Jeanne B.

2014-01-01

SUMMARY Recent studies recognize a vast diversity of non-coding RNAs with largely unknown functions, but few have examined interspersed repeat sequences, which constitute almost half our genome. RNA hybridization in situ using CoT-1 (highly repeated) DNA probes detects surprisingly abundant euchromatin-associated RNA comprised predominantly of repeat sequences (“CoT-1 RNA”), including LINE-1. CoT-1-hybridizing RNA strictly localizes to the interphase chromosome territory in cis, and remains stably associated with the chromosome territory following prolonged transcriptional inhibition. The CoT-1 RNA territory resists mechanical disruption and fractionates with the non-chromatin scaffold, but can be experimentally released. Loss of repeat-rich, stable nuclear RNAs from euchromatin corresponds to aberrant chromatin distribution and condensation. CoT-1 RNA has several properties similar to XIST chromosomal RNA, but is excluded from chromatin condensed by XIST. These findings impact two “black boxes” of genome science: the poorly understood diversity of non-coding RNA and the unexplained abundance of repetitive elements. PMID:24581492

COTS Initiative Panel Discussion

NASA Image and Video Library

2013-11-13

NASA Administrator Charles Bolden delivers remarks before a panel discussion on the Commercial Orbital Transportation Services (COTS) initiative at NASA Headquarters in Washington on Wednesday, November 13, 2013. Through COTS, NASA's partners Space Exploration Technologies Corp. (SpaceX) and Orbital Sciences Corp., developed new U.S. rockets and spacecraft, launched from U.S. soil, capable of transporting cargo to low-Earth orbit and the International Space Station. Photo Credit: (NASA/Jay Westcott)

COTS Initiative Panel Discussion

NASA Image and Video Library

2013-11-13

Gwynne Shotwell, President of SpaceX, delivers remarks panel discussion on the Commercial Orbital Transportation Services (COTS) initiative at NASA Headquarters in Washington on Wednesday, November 13, 2013. Through COTS, NASA's partners Space Exploration Technologies Corp. (SpaceX) and Orbital Sciences Corp., developed new U.S. rockets and spacecraft, launched from U.S. soil, capable of transporting cargo to low-Earth orbit and the International Space Station. Photo Credit: (NASA/Jay Westcott)

Magnetic blocking at 10 K and a dipolar-mediated avalanche in salts of the bis(η8-cyclooctatetraenide) complex [Er(COT)2]-.

PubMed

Meihaus, Katie R; Long, Jeffrey R

2013-11-27

The structures and magnetic properties of [K(18-crown-6)](+) (1) and [K(18-crown-6)(THF)2](+) (2) salts of the η(8)-cyclooctatetraenide sandwich complex [Er(COT)2](-) (COT(2-) = cyclooctatetraene dianion) are reported. Despite slight differences in symmetry, both compounds exhibit slow magnetic relaxation under zero applied dc field with relaxation barriers of ∼150 cm(-1) and waist-restricted magnetic hysteresis. Dc relaxation and dilution studies suggest that the drop in the magnetic hysteresis near zero field is influenced by a bulk magnetic avalanche effect coupled with tunneling of the magnetization. Through dilution with [K(18-crown-6)(THF)2][Y(COT)2] (3), these phenomena are substantially quenched, resulting in an open hysteresis loop to 10 K. Importantly, this represents the highest blocking temperature yet observed for a mononuclear complex and the second highest for any single-molecule magnet. A comprehensive comparative analysis of the magnetism of [K(18-crown-6)][Ln(COT)2] (Ln = Sm, Tb, Dy, Ho, Yb) reveals slow relaxation only for [K(18-crown-6)][Dy(COT)2] (4) with weak temperature dependence. Collectively, these results highlight the utility of an equatorial ligand field for facilitating slow magnetic relaxation in the prolate Er(III) ion.

Signal transduction mechanisms of K+-Cl- cotransport regulation and relationship to disease.

PubMed

Adragna, N C; Ferrell, C M; Zhang, J; Di Fulvio, M; Temprana, C F; Sharma, A; Fyffe, R E W; Cool, D R; Lauf, P K

2006-01-01

The K+-Cl- cotransport (COT) regulatory pathways recently uncovered in our laboratory and their implication in disease state are reviewed. Three mechanisms of K+-Cl- COT regulation can be identified in vascular cells: (1) the Li+-sensitive pathway, (2) the platelet-derived growth factor (PDGF)-sensitive pathway and (3) the nitric oxide (NO)-dependent pathway. Ion fluxes, Western blotting, semi-quantitative RT-PCR, immunofluorescence and confocal microscopy were used. Li+, used in the treatment of manic depression, stimulates volume-sensitive K+-Cl- COT of low K+ sheep red blood cells at cellular concentrations <1 mM and inhibits at >3 mM, causes cell swelling, and appears to regulate K+-Cl- COT through a protein kinase C-dependent pathway. PDGF, a potent serum mitogen for vascular smooth muscle cells (VSMCs), regulates membrane transport and is involved in atherosclerosis. PDGF stimulates VSM K+-Cl- COT in a time- and concentration-dependent manner, both acutely and chronically, through the PDGF receptor. The acute effect occurs at the post-translational level whereas the chronic effect may involve regulation through gene expression. Regulation by PDGF involves the signalling molecules phosphoinositides 3-kinase and protein phosphatase-1. Finally, the NO/cGMP/protein kinase G pathway, involved in vasodilation and hence cardiovascular disease, regulates K+-Cl- COT in VSMCs at the mRNA expression and transport levels. A complex and diverse array of mechanisms and effectors regulate K+-Cl- COT and thus cell volume homeostasis, setting the stage for abnormalities at the genetic and/or regulatory level thus effecting or being affected by various pathological conditions.

The Crystal Structure of Cancer Osaka Thyroid Kinase Reveals an Unexpected Kinase Domain Fold*

PubMed Central

Gutmann, Sascha; Hinniger, Alexandra; Fendrich, Gabriele; Drückes, Peter; Antz, Sylvie; Mattes, Henri; Möbitz, Henrik; Ofner, Silvio; Schmiedeberg, Niko; Stojanovic, Aleksandar; Rieffel, Sebastien; Strauss, André; Troxler, Thomas; Glatthar, Ralf; Sparrer, Helmut

2015-01-01

Macrophages are important cellular effectors in innate immune responses and play a major role in autoimmune diseases such as rheumatoid arthritis. Cancer Osaka thyroid (COT) kinase, also known as mitogen-activated protein kinase kinase kinase 8 (MAP3K8) and tumor progression locus 2 (Tpl-2), is a serine-threonine (ST) kinase and is a key regulator in the production of pro-inflammatory cytokines in macrophages. Due to its pivotal role in immune biology, COT kinase has been identified as an attractive target for pharmaceutical research that is directed at the discovery of orally available, selective, and potent inhibitors for the treatment of autoimmune disorders and cancer. The production of monomeric, recombinant COT kinase has proven to be very difficult, and issues with solubility and stability of the enzyme have hampered the discovery and optimization of potent and selective inhibitors. We developed a protocol for the production of recombinant human COT kinase that yields pure and highly active enzyme in sufficient yields for biochemical and structural studies. The quality of the enzyme allowed us to establish a robust in vitro phosphorylation assay for the efficient biochemical characterization of COT kinase inhibitors and to determine the x-ray co-crystal structures of the COT kinase domain in complex with two ATP-binding site inhibitors. The structures presented in this study reveal two distinct ligand binding modes and a unique kinase domain architecture that has not been observed previously. The structurally versatile active site significantly impacts the design of potent, low molecular weight COT kinase inhibitors. PMID:25918157

A survey of COTS wireless transceiver solutions for unmanned/unattended homeland defense systems

NASA Astrophysics Data System (ADS)

Wurth, Timothy J.; Wells, Jeffrey S.; Trimble, Michael L.

2004-08-01

As the war on terrorism continues abroad, the need to maintain security at home remains a major concern. Every aspect of the security effort can benefit from rapid information transfer. Advances in Commercial-Off-the-Shelf (COTS) radios make them valuable in applications ranging from deployed sensors on unsecured borders, to organizing an emergency response team. Additionally, the reduced cost and availability of these transceivers make them a viable alternative to custom developments. As an example, COTS radios can serve as the communication element in Unattended Ground Sensor (UGS) and munitions systems. Other applications include networked sonobuoys and networked RFID tracking. An additional advantage of COTS solutions is that they are available in a small form factor. They have extremely small mechanical outlines and are easily installed in systems requiring miniature designs and light payloads such as man-pack emplaced radios for the Army"s Future Combat System (FCS), as well as missions carried out by Unmanned Aerial Vehicles (UAV). The availability of the COTS radios result in a low-cost alternative for communication links that are applicable to military and Homeland Defense projects. They provide an easily obtainable, low-cost radio alternative enabling quick design cycles to meet mission timeliness. COTS solutions eliminate months from a typical design cycle and have many features already implemented such as ad-hoc routing and encryption. This enables robust hardware to be fielded quickly when a new need arises. The focus of this paper is to identify different COTS modules that can efficiently and cost effectively be applied to these and other various applications.

Low-cost real-time infrared scene generation for image projection and signal injection

NASA Astrophysics Data System (ADS)

Buford, James A., Jr.; King, David E.; Bowden, Mark H.

1998-07-01

As cost becomes an increasingly important factor in the development and testing of Infrared sensors and flight computer/processors, the need for accurate hardware-in-the- loop (HWIL) simulations is critical. In the past, expensive and complex dedicated scene generation hardware was needed to attain the fidelity necessary for accurate testing. Recent technological advances and innovative applications of established technologies are beginning to allow development of cost-effective replacements for dedicated scene generators. These new scene generators are mainly constructed from commercial-off-the-shelf (COTS) hardware and software components. At the U.S. Army Aviation and Missile Command (AMCOM) Missile Research, Development, and Engineering Center (MRDEC), researchers have developed such a dynamic IR scene generator (IRSG) built around COTS hardware and software. The IRSG is used to provide dynamic inputs to an IR scene projector for in-band seeker testing and for direct signal injection into the seeker or processor electronics. AMCOM MRDEC has developed a second generation IRSG, namely IRSG2, using the latest Silicon Graphics Incorporated (SGI) Onyx2 with Infinite Reality graphics. As reported in previous papers, the SGI Onyx Reality Engine 2 is the platform of the original IRSG that is now referred to as IRSG1. IRSG1 has been in operation and used daily for the past three years on several IR projection and signal injection HWIL programs. Using this second generation IRSG, frame rates have increased from 120 Hz to 400 Hz and intensity resolution from 12 bits to 16 bits. The key features of the IRSGs are real time missile frame rates and frame sizes, dynamic missile-to-target(s) viewpoint updated each frame in real-time by a six-degree-of- freedom (6DOF) system under test (SUT) simulation, multiple dynamic objects (e.g. targets, terrain/background, countermeasures, and atmospheric effects), latency compensation, point-to-extended source anti-aliased targets, and sensor modeling effects. This paper provides a comparison between the IRSG1 and IRSG2 systems and focuses on the IRSG software, real time features, and database development tools.

Chronic opioid therapy risk reduction initiative: impact on urine drug testing rates and results.

PubMed

Turner, Judith A; Saunders, Kathleen; Shortreed, Susan M; Rapp, Suzanne E; Thielke, Stephen; LeResche, Linda; Riddell, Kim M; Von Korff, Michael

2014-02-01

In response to epidemic levels of prescription opioid overdose, abuse, and diversion, routine urine drug tests (UDTs) are recommended for patients receiving chronic opioid therapy (COT) for chronic pain. However, UDT ordering for COT patients is inconsistent in primary care, and little is known about how to increase UDT ordering or the impact of increased testing on rates of aberrant results. To compare rates and results of UDTs for COT patients before versus after implementation of an opioid risk reduction initiative in a large healthcare system. Pre-post observational study. Group Health patients on COT October 2008-September 2009 (N = 4,821), October 2009-September 2010 (N = 5,081), and October 2010-September 2011 (N = 5,498). Multi-faceted opioid risk reduction initiative. Annual rates of UDTs and UDT results. Half of COT patients received at least one UDT in the year after the initiative was implemented, compared to only 7 % 2 years prior. The adjusted odds of COT patients having at least one UDT in the first year of the opioid initiative were almost 16 times (adjusted OR = 15.79; 95 % CI: 13.96-17.87) those 2 years prior. The annual rate of UDT detection of marijuana and illicit drugs did not change (12.6 % after initiative implementation), and largely reflected marijuana use (detected in 11.1 % of all UDTs in the year after initiative implementation). In the year after initiative implementation, 10.7 % of UDTs were negative for opioids. The initiative appeared to dramatically increase urine drug testing of COT patients in the healthcare system without impacting rates of aberrant results. The large majority of aberrant results reflected marijuana use or absence of opioids in the urine. The utility of increased urine drug testing for COT patient safety and prevention of diversion remains uncertain.

COTS Silicon diodes as radiation detectors in proton and heavy charged particle radiotherapy 1.

PubMed

Kaiser, Franz-Joachim; Bassler, Niels; Jäkel, Oliver

2010-08-01

Modern radiotherapy facilities for cancer treatment such as the Heavy Ion Therapy Center (HIT) in Heidelberg, Germany, allow for sub-millimeter precision in dose deposition. For measurement of such dose distributions and characterization of the particle beams, detectors with high spatial resolution are necessary. Here, a detector based on the commercially available COTS photodiode (BPW-34) is presented. When applied in hadronic beams of protons and carbon ions, the detector reproduces dose distribution well, but its response decreases rapidly by radiation damage. However, for MeV photon beams, the detector exhibits a similar behavior as found in diode detectors usually applied in radiotherapy.

Design of an Eye Limiting Resolution Visual System Using Commercial-Off-the-Shelf Equipment

NASA Technical Reports Server (NTRS)

Sweet, Barbara T.; Giovannetti, Dean P.

2008-01-01

A feasibility study was conducted to determine if a flight simulator with an eye-limiting resolution out-the-window (OTW) visual system could be built using commercial off-the-shelf (COTS) technology and used to evaluate the visual performance of Air Force pilots in an operations context. Results of this study demonstrate that an eye limiting OTW visual system can be built using COTS technology. Further, a series of operationally-based tasks linked to clinical vision tests can be used within the synthetic environment to demonstrate a correlation and quantify the level of correlation between vision and operational aviation performance.

Design Considerations for Miniaturized Control Moment Gyroscopes for Rapid Retargeting and Precision Pointing of Small Satellites

NASA Technical Reports Server (NTRS)

Patankar, Kunal; Fitz-Coy, Norman; Roithmayr, Carlos M.

2014-01-01

This paper presents the design as well as characterization of a practical control moment gyroscope (CMG) based attitude control system (ACS) for small satellites in the 15-20 kilogram mass range performing rapid retargeting and precision pointing maneuvers. The paper focuses on the approach taken in the design of miniaturized CMGs while considering the constraints imposed by the use of commercial off-the-shelf (COTS) components as well as the size of the satellite. It is shown that a hybrid mode is more suitable for COTS based moment exchange actuators; a mode that uses the torque amplification of CMGs for rapid retargeting and direct torque capabilities of the flywheel motors for precision pointing. A simulation is provided to demonstrate on-orbit slew and pointing performance.

How to Boost Engineering Support Via Web 2.0 - Seeds for the Ares Project...and/or Yours?

NASA Technical Reports Server (NTRS)

Scott, David W.

2010-01-01

The Mission Operations Laboratory (MOL) at Marshall Space Flight Center (MSFC) is responsible for Engineering Support capability for NASA s Ares launch system development. In pursuit of this, MOL is building the Ares Engineering and Operations Network (AEON), a web-based portal intended to provide a seamless interface to support and simplify two critical activities: a) Access and analyze Ares manufacturing, test, and flight performance data, with access to Shuttle data for comparison. b) Provide archive storage for engineering instrumentation data to support engineering design, development, and test. A mix of NASA-written and COTS software provides engineering analysis tools. A by-product of using a data portal to access and display data is access to collaborative tools inherent in a Web 2.0 environment. This paper discusses how Web 2.0 techniques, particularly social media, might be applied to the traditionally conservative and formal engineering support arena. A related paper by the author [1] considers use

ACS sampling system: design, implementation, and performance evaluation

NASA Astrophysics Data System (ADS)

Di Marcantonio, Paolo; Cirami, Roberto; Chiozzi, Gianluca

2004-09-01

By means of ACS (ALMA Common Software) framework we designed and implemented a sampling system which allows sampling of every Characteristic Component Property with a specific, user-defined, sustained frequency limited only by the hardware. Collected data are sent to various clients (one or more Java plotting widgets, a dedicated GUI or a COTS application) using the ACS/CORBA Notification Channel. The data transport is optimized: samples are cached locally and sent in packets with a lower and user-defined frequency to keep network load under control. Simultaneous sampling of the Properties of different Components is also possible. Together with the design and implementation issues we present the performance of the sampling system evaluated on two different platforms: on a VME based system using VxWorks RTOS (currently adopted by ALMA) and on a PC/104+ embedded platform using Red Hat 9 Linux operating system. The PC/104+ solution offers, as an alternative, a low cost PC compatible hardware environment with free and open operating system.

Spacecraft Avionics Software Development Then and Now: Different but the Same

NASA Technical Reports Server (NTRS)

Mangieri, Mark L.; Garman, John (Jack); Vice, Jason

2012-01-01

NASA has always been in the business of balancing new technologies and techniques to achieve human space travel objectives. NASA s historic Software Production Facility (SPF) was developed to serve complex avionics software solutions during an era dominated by mainframes, tape drives, and lower level programming languages. These systems have proven themselves resilient enough to serve the Shuttle Orbiter Avionics life cycle for decades. The SPF and its predecessor the Software Development Lab (SDL) at NASA s Johnson Space Center (JSC) hosted flight software (FSW) engineering, development, simulation, and test. It was active from the beginning of Shuttle Orbiter development in 1972 through the end of the shuttle program in the summer of 2011 almost 40 years. NASA s Kedalion engineering analysis lab is on the forefront of validating and using many contemporary avionics HW/SW development and integration techniques, which represent new paradigms to NASA s heritage culture in avionics software engineering. Kedalion has validated many of the Orion project s HW/SW engineering techniques borrowed from the adjacent commercial aircraft avionics environment, inserting new techniques and skills into the Multi-Purpose Crew Vehicle (MPCV) Orion program. Using contemporary agile techniques, COTS products, early rapid prototyping, in-house expertise and tools, and customer collaboration, NASA has adopted a cost effective paradigm that is currently serving Orion effectively. This paper will explore and contrast differences in technology employed over the years of NASA s space program, due largely to technological advances in hardware and software systems, while acknowledging that the basic software engineering and integration paradigms share many similarities.

US NDC Modernization Iteration E1 Prototyping Report: Processing Control Framework

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prescott, Ryan; Hamlet, Benjamin R.

2014-12-01

During the first iteration of the US NDC Modernization Elaboration phase (E1), the SNL US NDC modernization project team developed an initial survey of applicable COTS solutions, and established exploratory prototyping related to the processing control framework in support of system architecture definition. This report summarizes these activities and discusses planned follow-on work.

A UML-based metamodel for software evolution process

NASA Astrophysics Data System (ADS)

Jiang, Zuo; Zhou, Wei-Hong; Fu, Zhi-Tao; Xiong, Shun-Qing

2014-04-01

A software evolution process is a set of interrelated software processes under which the corresponding software is evolving. An object-oriented software evolution process meta-model (OO-EPMM), abstract syntax and formal OCL constraint of meta-model are presented in this paper. OO-EPMM can not only represent software development process, but also represent software evolution.

COTS Initiative Panel Discussion

NASA Image and Video Library

2013-11-13

Alan Lindenmoyer, Manager of Commercial Crew and Cargo Program at NASA, delivers remarks panel discussion on the Commercial Orbital Transportation Services (COTS) initiative at NASA Headquarters in Washington on Wednesday, November 13, 2013. Through COTS, NASA's partners Space Exploration Technologies Corp. (SpaceX) and Orbital Sciences Corp., developed new U.S. rockets and spacecraft, launched from U.S. soil, capable of transporting cargo to low-Earth orbit and the International Space Station. Photo Credit: (NASA/Jay Westcott)

COTS Initiative Panel Discussion

NASA Image and Video Library

2013-11-13

Phil McAlister, Director of Commercial Spaceflight Development at NASA, delivers remarks panel discussion on the Commercial Orbital Transportation Services (COTS) initiative at NASA Headquarters in Washington on Wednesday, November 13, 2013. Through COTS, NASA's partners Space Exploration Technologies Corp. (SpaceX) and Orbital Sciences Corp., developed new U.S. rockets and spacecraft, launched from U.S. soil, capable of transporting cargo to low-Earth orbit and the International Space Station. Photo Credit: (NASA/Jay Westcott)

COTS Initiative Panel Discussion

NASA Image and Video Library

2013-11-13

Frank Slazer, Vice President of Space Systems, Aerospace Industries Association, delivers remarks panel discussion on the Commercial Orbital Transportation Services (COTS) initiative at NASA Headquarters in Washington on Wednesday, November 13, 2013. Through COTS, NASA's partners Space Exploration Technologies Corp. (SpaceX) and Orbital Sciences Corp., developed new U.S. rockets and spacecraft, launched from U.S. soil, capable of transporting cargo to low-Earth orbit and the International Space Station. Photo Credit: (NASA/Jay Westcott)

Fault-Tolerant, Radiation-Hard DSP

NASA Technical Reports Server (NTRS)

Czajkowski, David

2011-01-01

Commercial digital signal processors (DSPs) for use in high-speed satellite computers are challenged by the damaging effects of space radiation, mainly single event upsets (SEUs) and single event functional interrupts (SEFIs). Innovations have been developed for mitigating the effects of SEUs and SEFIs, enabling the use of very-highspeed commercial DSPs with improved SEU tolerances. Time-triple modular redundancy (TTMR) is a method of applying traditional triple modular redundancy on a single processor, exploiting the VLIW (very long instruction word) class of parallel processors. TTMR improves SEU rates substantially. SEFIs are solved by a SEFI-hardened core circuit, external to the microprocessor. It monitors the health of the processor, and if a SEFI occurs, forces the processor to return to performance through a series of escalating events. TTMR and hardened-core solutions were developed for both DSPs and reconfigurable field-programmable gate arrays (FPGAs). This includes advancement of TTMR algorithms for DSPs and reconfigurable FPGAs, plus a rad-hard, hardened-core integrated circuit that services both the DSP and FPGA. Additionally, a combined DSP and FPGA board architecture was fully developed into a rad-hard engineering product. This technology enables use of commercial off-the-shelf (COTS) DSPs in computers for satellite and other space applications, allowing rapid deployment at a much lower cost. Traditional rad-hard space computers are very expensive and typically have long lead times. These computers are either based on traditional rad-hard processors, which have extremely low computational performance, or triple modular redundant (TMR) FPGA arrays, which suffer from power and complexity issues. Even more frustrating is that the TMR arrays of FPGAs require a fixed, external rad-hard voting element, thereby causing them to lose much of their reconfiguration capability and in some cases significant speed reduction. The benefits of COTS high-performance signal processing include significant increase in onboard science data processing, enabling orders of magnitude reduction in required communication bandwidth for science data return, orders of magnitude improvement in onboard mission planning and critical decision making, and the ability to rapidly respond to changing mission environments, thus enabling opportunistic science and orders of magnitude reduction in the cost of mission operations through reduction of required staff. Additional benefits of COTS-based, high-performance signal processing include the ability to leverage considerable commercial and academic investments in advanced computing tools, techniques, and infra structure, and the familiarity of the science and IT community with these computing environments.

Chronic pre-operative opioid use is a risk factor for increased complications, resource use and costs after cervical fusion.

PubMed

Jain, Nikhil; Brock, John L; Phillips, Frank M; Weaver, Tristan; Khan, Safdar N

2018-04-27

As healthcare transitions to value-based models, there has been an increased focus on patient factors that can influence peri- and post-operative adverse events, resource use, and costs. Many studies have reported risk factors for systemic complications after cervical fusion, but none have studied chronic opioid therapy (COT) as a risk factor. To answer the following questions from a large cohort of patients who underwent primary cervical fusion for degenerative pathology: (1) What is the patient profile associated with pre-operative COT? (2) Is pre-operative COT a risk factor for 90-day systemic complications, emergency department (ED) visits, readmission, and one-year adverse events? (3) What are the risk factors and one-year adverse events related to long-term post-operative opioid use? and (4) How much did payers reimburse for management of complications and adverse events? Retrospective review of Humana commercial insurance data (2007-Q3 2015). 29,101 patients undergoing primary cervical fusion for degenerative pathology. Patients and procedures of interest were included using International Classification of Diseases (ICD) coding. Patients with opioid prescriptions for >6 months before surgery were considered as having pre-operative COT. Patients with continued opioid use till one-year after surgery were considered as long-term users. Descriptive analysis of patient cohorts has been done. Multiple-variable logistic regression analyses adjusting for approach, number of levels of surgery, discharge disposition, and comorbidities were done to answer first three study questions. Reimbursement data from insurers has been reported to answer our fourth study question. Of the entire cohort, 6,643 (22.8%) had pre-operative COT. Pre-operative COT was associated with a higher risk of 90-day wound complications (OR 1.39, 95% CI:1.16-1.66), all-cause 90-day ED visits (adjusted OR 1.22, 95% CI:1.13-1.32), and pain-related ED visits (adjusted OR 1.39, 95% CI:1.24-1.55). Patients who had pre-operative COT were more likely to receive epidural and/or facet joint injections within one-year after surgery (adjusted OR 1.68, 95% CI: 1.47-1.92). These patients were also more likely to undergo a repeat cervical fusion within a year as compared to patients who did not have pre-operative COT (adjusted OR 1.21, 95% CI: 1.01-1.43). Pre-operative COT had a higher likelihood of long-term use after surgery (adjusted OR 4.72, 95% CI:4.41-5.06). Long-term opioid use after surgery was associated with a higher risk of new-onset constipation (adjusted OR 1.34, 95% CI:1.22-1.48). The risk of complications and adverse events was not found to be significant in patients with < 3-months pre-operative opioid use or those who stopped opioids for at-least 6-weeks before surgery. The cost of additional resource use for medications, ED visits, constipation, injections and revision fusion ranged from $623-$27,360 per patient. Pre-operative opioid use among cervical fusion patients increases complication rates, post-operative opioid usage, healthcare resource utilization and costs. These risks may be reduced by restricting the duration of pre-operative opioid use or weaning off before surgery. Better understanding and management of pain in the pre-operative period with judicious use of opioids is critical to enhance outcomes after cervical fusion surgery. Copyright © 2018 Elsevier Inc. All rights reserved.

A COTS-Style Acquisition Strategy for Human Exploration Beyond LEO

NASA Technical Reports Server (NTRS)

Arney, Dale; Earle, Kevin; Klovstad, Jordan; Jones, Christopher

2016-01-01

The Evolvable Mars Campaign presents a long term strategy for NASA's Journey to Mars within a capability driven framework. By comparing each element to a set of criteria, this paper reviews the potential of acquiring those capabilities using a strategy similar to the Commercial Orbital Transportation Services program. The paper presents the criteria, assesses the elements against those criteria, and then discusses the suitability of each element to being developed using this acquisition strategy. Throughout the campaign, certain capabilities are well suited to being developed in this manner while others are not. This assessment is a snapshot in time, and should be revisited as the campaign and/or commercial capabilities change. This paper will explore each of these elements in the campaign and discuss how the COTS development andacquisition strategy could or could not be applied to those elements. This assessment will be based on theservices or functionality required in the campaign, and will use the best practices discussed above to create acase for or against a COTS-style acquisition strategy for each given element.

Signal Acquisition Using AXIe

NASA Astrophysics Data System (ADS)

Narciso, Steven J.

2011-08-01

An emerging test and measurement standard called AXIe, AdvancedTCA extensions for Instrumentation, is expected to find wide acceptance within the Physics community as it offers many benefits to applications including shock, plasma, particle and nuclear physics. It is expected that many COTS (commercial off-the-shelf) signal conditioning, acquisition and processing modules will become available from a range of different suppliers. AXIe uses AdvancedTCA® as its basis, but then levers test and measurement industry standards such as PXI, IVI, and LXI to facilitate cooperation and plug-and-play interoperability between COTS instrument suppliers. AXIe's large board footprint and power allows high density in a 19" rack, enabling the development of high-performance signal conditioning, analog-to-digital conversion, and data processing, while offering channel count scalability inherent in modular systems. Synchronization between modules is flexible and provided by two triggering structures: a parallel trigger bus, and radially-distributed, time-matched point-to-point trigger lines. Inter-module communication is also provided with an adjacent module local bus allowing data transfer to 600 Gbits/s in each direction, for example between a front-end digitizer and DSP. AXIe allows embedding high performance computing and a range of COTS AdvancedTCA® computer blades are currently available that provide low cost alternatives to the development of custom signal processing modules. The availability of both LAN and PCI Express allow interconnection between modules, as well as industry-standard high-performance data paths to external host computer systems. AXIe delivers a powerful environment for custom module devel opment. As in the case of VXIbus and PXI before it, commercial development kits are expected to be available. This paper will give an overview of the architectural elements of AXIe 1.0, the compatibility model with AdvancedTCA, and signal acquisition performance of many of the AXIe structures.

Computers for Manned Space Applications Base on Commercial Off-the-Shelf Components

NASA Astrophysics Data System (ADS)

Vogel, T.; Gronowski, M.

2009-05-01

Similar to the consumer markets there has been an ever increasing demand in processing power, signal processing capabilities and memory space also for computers used for science data processing in space. An important driver of this development have been the payload developers for the International Space Station, requesting high-speed data acquisition and fast control loops in increasingly complex systems. Current experiments now even perform video processing and compression with their payload controllers. Nowadays the requirements for a space qualified computer are often far beyond the capabilities of, for example, the classic SPARC architecture that is found in ERC32 or LEON CPUs. An increase in performance usually demands costly and power consuming application specific solutions. Continuous developments over the last few years have now led to an alternative approach that is based on complete electronics modules manufactured for commercial and industrial customers. Computer modules used in industrial environments with a high demand for reliability under harsh environmental conditions like chemical reactors, electrical power plants or on manufacturing lines are entered into a selection procedure. Promising candidates then undergo a detailed characterisation process developed by Astrium Space Transportation. After thorough analysis and some modifications, these modules can replace fully qualified custom built electronics in specific, although not safety critical applications in manned space. This paper focuses on the benefits of COTS1 based electronics modules and the necessary analyses and modifications for their utilisation in manned space applications on the ISS. Some considerations regarding overall systems architecture will also be included. Furthermore this paper will also pinpoint issues that render such modules unsuitable for specific tasks, and justify the reasons. Finally, the conclusion of this paper will advocate the implementation of COTS based electronics for a range of applications within specifically adapted systems. The findings in this paper are extrapolated from two reference computer systems, both having been launched in 2008. One of those was a LEON-2 based computer installed onboard the Columbus Orbital Facility while the other system consisted mainly of a commercial Power-PC module that was modified for a launch mounted on the ICC pallet in the Space Shuttle's cargo bay. Both systems are currently upgraded and extended for future applications.

Factors affecting metabolic cost of transport during a multi-stage running race.

PubMed

Lazzer, Stefano; Taboga, Paolo; Salvadego, Desy; Rejc, Enrico; Simunic, Bostjan; Narici, Marco V; Buglione, Antonio; Giovanelli, Nicola; Antonutto, Guglielmo; Grassi, Bruno; Pisot, Rado; di Prampero, Pietro E

2014-03-01

The aim of this study was to investigate: (1) the role of , the fraction of (F) and the metabolic cost of transport (CoT) in determining performance during an ultra-endurance competition and (2) the effects of the race on several biomechanical and morphological parameters of the lower limbs that are likely to affect CoT. Eleven runners (aged 29-54 years) participated in an ultra-endurance competition consisting of three running stages of 25, 55 and 13 km on three consecutive days. Anthropometric characteristics, body composition, morphological properties of the gastrocnemius medialis, maximal explosive power of the lower limb and were determined before the competition. In addition, biomechanics of running and CoT were determined, before and immediately after each running stage. Performance was directly proportional to (r=0.77) and F (r=0.36), and inversely proportional to CoT (r=-0.30). Low CoT values were significantly related to high maximal power of the lower limbs (r=-0.74) and vertical stiffness (r=-0.65) and low footprint index (FPI, r=0.70), step frequency (r=0.62) and external work (r=0.60). About 50% of the increase in CoT during the stages of the competition was accounted for by changes in FPI, which represents a global evaluation of medio-lateral displacement of the foot during the whole stance phase, which in turn is associated with the myotendinous characteristics of the lower limb. Thus, lower CoT values were related to greater muscular power and lower FPI, suggesting that a better ankle stability is likely to achieve better performance in an ultra-endurance running competition.

Comparison of antibiotic prophylaxis with cotrimoxazole/colistin (COT/COL) versus ciprofloxacin (CIP) in patients with acute myeloid leukemia.

PubMed

Mayer, Karin; Hahn-Ast, Corinna; Mückter, Sara; Schmitz, Andrea; Krause, Simon; Felder, Linda; Bekeredjian-Ding, Isabelle; Molitor, Ernst; Brossart, Peter; von Lilienfeld-Toal, Marie

2015-05-01

Recent meta-analyses showed that antibiotic prophylaxis in patients with neutropenia after chemotherapy reduced the incidence of fever and mortality rate. Fluoroquinolones appear to be most effective and well tolerated. Thus, in April 2008, we changed our antibiotic prophylaxis regimen from cotrimoxazole/colistin (COT/COL) to the fluoroquinolone ciprofloxacin (CIP) in patients with acute myeloid leukemia (AML). The aim of this retrospective study was to compare efficacy and development of bacterial resistance with two different prophylaxis regimens over a time period of more than 4 years. Induction chemotherapy courses given for AML during the antibiotic prophylaxis period with COT/COL (01/2006-04/2008) and CIP (04/2008-06/2010) were retrospectively analyzed with a standard questionnaire. Eighty-five courses in the COT/COL group and 105 in the CIP group were analyzed. The incidence of fever was not significantly different (COT/COL 80 % vs CIP 77 %; p = 0.724). Also, the rate of microbiologically documented infections was nearly the same (29 vs 26 %; p = 0.625). In addition, there was no significant difference in the incidence of clinically documented infections (11 vs 19 %; p = 0.155) or in the rates of detected gram-positive and gram-negative bacteria. Of note, there was no increase in resistance rates or cases with Clostridium difficile-associated diarrhea in the CIP group. The antibiotic prophylaxis with CIP compared to COT/COL in AML was similarly effective with no increase in bacterial resistance. COT/COL may have the advantages of providing additional prophylaxis against Pneumocystis jirovecii pneumonia and leaving fluoroquinolones as an additional option for treatment of febrile neutropenia.

Accounting for genetic and environmental confounds in associations between parent and child characteristics: a systematic review of children-of-twins studies.

PubMed

McAdams, Tom A; Neiderhiser, Jenae M; Rijsdijk, Fruhling V; Narusyte, Jurgita; Lichtenstein, Paul; Eley, Thalia C

2014-07-01

Parental psychopathology, parenting style, and the quality of intrafamilial relationships are all associated with child mental health outcomes. However, most research can say little about the causal pathways underlying these associations. This is because most studies are not genetically informative and are therefore not able to account for the possibility that associations are confounded by gene-environment correlation. That is, biological parents not only provide a rearing environment for their child, but also contribute 50% of their genes. Any associations between parental phenotype and child phenotype are therefore potentially confounded. One technique for disentangling genetic from environmental effects is the children-of-twins (COT) method. This involves using data sets comprising twin parents and their children to distinguish genetic from environmental associations between parent and child phenotypes. The COT technique has grown in popularity in the last decade, and we predict that this surge in popularity will continue. In the present article we explain the COT method for those unfamiliar with its use. We present the logic underlying this approach, discuss strengths and weaknesses, and highlight important methodological considerations for researchers interested in the COT method. We also cover variations on basic COT approaches, including the extended-COT method, capable of distinguishing forms of gene-environment correlation. We then present a systematic review of all the behavioral COT studies published to date. These studies cover such diverse phenotypes as psychosis, substance abuse, internalizing, externalizing, parenting, and marital difficulties. In reviewing this literature, we highlight past applications, identify emergent patterns, and suggest avenues for future research. PsycINFO Database Record (c) 2014 APA, all rights reserved.

A longitudinal study of depression among middle-aged and senior patients initiating chronic opioid therapy.

PubMed

Von Korff, Michael; Shortreed, Susan M; LeResche, Linda; Saunders, Kathleen; Thielke, Stephen; Thakral, Manu; Rosenberg, Dori; Turner, Judith A

2017-03-15

Improved understanding how depressive symptoms change with sustained opioid use is needed. We prospectively assessed patients 45 years or older initiating chronic opioid therapy (COT) at baseline and at 4 and 12 months, differentiating recent COT initiators (n=748) and continuing users (n=468). Level of opioid use before 12-month follow-up was classified as regular/higher-dose, intermittent/lower-dose, or minimal/no use. Depressive symptoms were assessed using the Patient Health Questionnaire-8 (PHQ-8). Depressive symptoms decreased, on average, from baseline to 12 months regardless of level of opioid use. COT patients with regular/higher-dose compared to those with intermittent/lower-dose opioid use (who had similar pain outcomes) did not differ in PHQ-8 scores at 12 months (adjusted mean difference -0.14, 95% CI, -1.07, 0.78 for COT initiators). At 12 months, COT patients with intermittent/lower-dose use had higher adjusted PHQ-8 scores than did those with minimal/no opioid use (adjusted mean difference 0.77, 95% CI, 0.03-1.52 for COT initiators). However, 77% of patients who discontinued opioids cited improved pain as a reason for discontinuation, while 21% cited negative emotional effects of opioids as a reason for discontinuation. Discontinuation was more common among persons who, at baseline, attributed 3 or more depressive symptoms to opioid use. Results are relevant to older COT patients receiving low to moderate opioid doses. Depressive symptoms did not increase with sustained opioid use. Depressive symptoms were not higher with regular/higher-dose compared to intermittent/lower-dose use. Persons who perceived negative effects of opioids on emotions more often discontinued their use. Copyright © 2017. Published by Elsevier B.V.

Development and interval testing of a naturalistic driving methodology to evaluate driving behavior in clinical research.

PubMed

Babulal, Ganesh M; Addison, Aaron; Ghoshal, Nupur; Stout, Sarah H; Vernon, Elizabeth K; Sellan, Mark; Roe, Catherine M

2016-01-01

Background : The number of older adults in the United States will double by 2056. Additionally, the number of licensed drivers will increase along with extended driving-life expectancy. Motor vehicle crashes are a leading cause of injury and death in older adults. Alzheimer's disease (AD) also negatively impacts driving ability and increases crash risk. Conventional methods to evaluate driving ability are limited in predicting decline among older adults. Innovations in GPS hardware and software can monitor driving behavior in the actual environments people drive in. Commercial off-the-shelf (COTS) devices are affordable, easy to install and capture large volumes of data in real-time. However, adapting these methodologies for research can be challenging. This study sought to adapt a COTS device and determine an interval that produced accurate data on the actual route driven for use in future studies involving older adults with and without AD.  Methods : Three subjects drove a single course in different vehicles at different intervals (30, 60 and 120 seconds), at different times of day, morning (9:00-11:59AM), afternoon (2:00-5:00PM) and night (7:00-10pm). The nine datasets were examined to determine the optimal collection interval. Results : Compared to the 120-second and 60-second intervals, the 30-second interval was optimal in capturing the actual route driven along with the lowest number of incorrect paths and affordability weighing considerations for data storage and curation. Discussion : Use of COTS devices offers minimal installation efforts, unobtrusive monitoring and discreet data extraction.  However, these devices require strict protocols and controlled testing for adoption into research paradigms.  After reliability and validity testing, these devices may provide valuable insight into daily driving behaviors and intraindividual change over time for populations of older adults with and without AD.  Data can be aggregated over time to look at changes or adverse events and ascertain if decline in performance is occurring.

Development of GK-2A cloud optical and microphysical properties retrieval algorithm

NASA Astrophysics Data System (ADS)

Yang, Y.; Yum, S. S.; Um, J.

2017-12-01

Cloud and aerosol radiative forcing is known to be one of the the largest uncertainties in climate change prediction. To reduce this uncertainty, remote sensing observation of cloud radiative and microphysical properties have been used since 1970s and the corresponding remote sensing techniques and instruments have been developed. As a part of such effort, Geo-KOMPSAT-2A (Geostationary Korea Multi-Purpose Satellite-2A, GK-2A) will be launched in 2018. On the GK-2A, the Advanced Meteorological Imager (AMI) is primary instrument which have 3 visible, 3 near-infrared, and 10 infrared channels. To retrieve optical and microphysical properties of clouds using AMI measurements, the preliminary version of new cloud retrieval algorithm for GK-2A was developed and several validation tests were conducted. This algorithm retrieves cloud optical thickness (COT), cloud effective radius (CER), liquid water path (LWP), and ice water path (IWP), so we named this algorithm as Daytime Cloud Optical thickness, Effective radius and liquid and ice Water path (DCOEW). The DCOEW uses cloud reflectance at visible and near-infrared channels as input data. An optimal estimation (OE) approach that requires appropriate a-priori values and measurement error information is used to retrieve COT and CER. LWP and IWP are calculated using empirical relationships between COT/CER and cloud water path that were determined previously. To validate retrieved cloud properties, we compared DCOEW output data with other operational satellite data. For COT and CER validation, we used two different data sets. To compare algorithms that use cloud reflectance at visible and near-IR channels as input data, MODIS MYD06 cloud product was selected. For the validation with cloud products that are based on microwave measurements, COT(2B-TAU)/CER(2C-ICE) data retrieved from CloudSat cloud profiling radar (W-band, 94 GHz) was used. For cloud water path validation, AMSR-2 Level-3 Cloud liquid water data was used. Detailed results will be shown at the conference.

Portable Airborne Laser System Measures Forest-Canopy Height

NASA Technical Reports Server (NTRS)

Nelson, Ross

2005-01-01

(PALS) is a combination of laser ranging, video imaging, positioning, and data-processing subsystems designed for measuring the heights of forest canopies along linear transects from tens to thousands of kilometers long. Unlike prior laser ranging systems designed to serve the same purpose, the PALS is not restricted to use aboard a single aircraft of a specific type: the PALS fits into two large suitcases that can be carried to any convenient location, and the PALS can be installed in almost any local aircraft for hire, thereby making it possible to sample remote forests at relatively low cost. The initial cost and the cost of repairing the PALS are also lower because the PALS hardware consists mostly of commercial off-the-shelf (COTS) units that can easily be replaced in the field. The COTS units include a laser ranging transceiver, a charge-coupled-device camera that images the laser-illuminated targets, a differential Global Positioning System (dGPS) receiver capable of operation within the Wide Area Augmentation System, a video titler, a video cassette recorder (VCR), and a laptop computer equipped with two serial ports. The VCR and computer are powered by batteries; the other units are powered at 12 VDC from the 28-VDC aircraft power system via a low-pass filter and a voltage converter. The dGPS receiver feeds location and time data, at an update rate of 0.5 Hz, to the video titler and the computer. The laser ranging transceiver, operating at a sampling rate of 2 kHz, feeds its serial range and amplitude data stream to the computer. The analog video signal from the CCD camera is fed into the video titler wherein the signal is annotated with position and time information. The titler then forwards the annotated signal to the VCR for recording on 8-mm tapes. The dGPS and laser range and amplitude serial data streams are processed by software that displays the laser trace and the dGPS information as they are fed into the computer, subsamples the laser range and amplitude data, interleaves the subsampled data with the dGPS information, and records the resulting interleaved data stream.

The Nuclear Dbf2-Related Kinase COT1 and the Mitogen-Activated Protein Kinases MAK1 and MAK2 Genetically Interact to Regulate Filamentous Growth, Hyphal Fusion and Sexual Development in Neurospora crassa

PubMed Central

Maerz, Sabine; Ziv, Carmit; Vogt, Nico; Helmstaedt, Kerstin; Cohen, Nourit; Gorovits, Rena; Yarden, Oded; Seiler, Stephan

2008-01-01

Ndr kinases, such as Neurospora crassa COT1, are important for cell differentiation and polar morphogenesis, yet their input signals as well as their integration into a cellular signaling context are still elusive. Here, we identify the cot-1 suppressor gul-4 as mak-2 and show that mutants of the gul-4/mak-2 mitogen-activated protein (MAP) kinase pathway suppress cot-1 phenotypes along with a concomitant reduction in protein kinase A (PKA) activity. Furthermore, mak-2 pathway defects are partially overcome in a cot-1 background and are associated with increased MAK1 MAPK signaling. A comparative characterization of N. crassa MAPKs revealed that they act as three distinct modules during vegetative growth and asexual development. In addition, common functions of MAK1 and MAK2 signaling during maintenance of cell-wall integrity distinguished the two ERK-type pathways from the p38-type OS2 osmosensing pathway. In contrast to separate functions during vegetative growth, the concerted activity of the three MAPK pathways is essential for cell fusion and for the subsequent formation of multicellular structures that are required for sexual development. Taken together, our data indicate a functional link between COT1 and MAPK signaling in regulating filamentous growth, hyphal fusion, and sexual development. PMID:18562669

NASA's Software Safety Standard

NASA Technical Reports Server (NTRS)

Ramsay, Christopher M.

2007-01-01

NASA relies more and more on software to control, monitor, and verify its safety critical systems, facilities and operations. Since the 1960's there has hardly been a spacecraft launched that does not have a computer on board that will provide command and control services. There have been recent incidents where software has played a role in high-profile mission failures and hazardous incidents. For example, the Mars Orbiter, Mars Polar Lander, the DART (Demonstration of Autonomous Rendezvous Technology), and MER (Mars Exploration Rover) Spirit anomalies were all caused or contributed to by software. The Mission Control Centers for the Shuttle, ISS, and unmanned programs are highly dependant on software for data displays, analysis, and mission planning. Despite this growing dependence on software control and monitoring, there has been little to no consistent application of software safety practices and methodology to NASA's projects with safety critical software. Meanwhile, academia and private industry have been stepping forward with procedures and standards for safety critical systems and software, for example Dr. Nancy Leveson's book Safeware: System Safety and Computers. The NASA Software Safety Standard, originally published in 1997, was widely ignored due to its complexity and poor organization. It also focused on concepts rather than definite procedural requirements organized around a software project lifecycle. Led by NASA Headquarters Office of Safety and Mission Assurance, the NASA Software Safety Standard has recently undergone a significant update. This new standard provides the procedures and guidelines for evaluating a project for safety criticality and then lays out the minimum project lifecycle requirements to assure the software is created, operated, and maintained in the safest possible manner. This update of the standard clearly delineates the minimum set of software safety requirements for a project without detailing the implementation for those requirements. This allows the projects leeway to meet these requirements in many forms that best suit a particular project's needs and safety risk. In other words, it tells the project what to do, not how to do it. This update also incorporated advances in the state of the practice of software safety from academia and private industry. It addresses some of the more common issues now facing software developers in the NASA environment such as the use of Commercial-Off-the-Shelf Software (COTS), Modified OTS (MOTS), Government OTS (GOTS), and reused software. A team from across NASA developed the update and it has had both NASA-wide internal reviews by software engineering, quality, safety, and project management. It has also had expert external review. This presentation and paper will discuss the new NASA Software Safety Standard, its organization, and key features. It will start with a brief discussion of some NASA mission failures and incidents that had software as one of their root causes. It will then give a brief overview of the NASA Software Safety Process. This will include an overview of the key personnel responsibilities and functions that must be performed for safety-critical software.

Software Dependability and Safety Evaluations ESA's Initiative

NASA Astrophysics Data System (ADS)

Hernek, M.

ESA has allocated funds for an initiative to evaluate Dependability and Safety methods of Software. The objectives of this initiative are; · More extensive validation of Safety and Dependability techniques for Software · Provide valuable results to improve the quality of the Software thus promoting the application of Dependability and Safety methods and techniques. ESA space systems are being developed according to defined PA requirement specifications. These requirements may be implemented through various design concepts, e.g. redundancy, diversity etc. varying from project to project. Analysis methods (FMECA. FTA, HA, etc) are frequently used during requirements analysis and design activities to assure the correct implementation of system PA requirements. The criticality level of failures, functions and systems is determined and by doing that the critical sub-systems are identified, on which dependability and safety techniques are to be applied during development. Proper performance of the software development requires the development of a technical specification for the products at the beginning of the life cycle. Such technical specification comprises both functional and non-functional requirements. These non-functional requirements address characteristics of the product such as quality, dependability, safety and maintainability. Software in space systems is more and more used in critical functions. Also the trend towards more frequent use of COTS and reusable components pose new difficulties in terms of assuring reliable and safe systems. Because of this, its dependability and safety must be carefully analysed. ESA identified and documented techniques, methods and procedures to ensure that software dependability and safety requirements are specified and taken into account during the design and development of a software system and to verify/validate that the implemented software systems comply with these requirements [R1].

Academic and Non-Profit Accessibility to Commercial Remote Sensing Software

NASA Astrophysics Data System (ADS)

O'Connor, A. S.; Farr, B.

2013-12-01

Remote Sensing as a topic of teaching and research at the university and college level continues to increase. As more data is made freely available and software becomes easier to use, more and more academic and non-profits institutions are turning to remote sensing to solve their tough and large spatial scale problems. Exelis Visual Information Solutions (VIS) has been supporting teaching and research endeavors for over 30 years with a special emphasis over the last 5 years with scientifically proven software and accessible training materials. The Exelis VIS academic program extends to US and Canadian 2 year and 4 year colleges and universities with tools for analyzing aerial and satellite multispectral and hyperspectral imagery, airborne LiDAR and Synthetic Aperture Radar. The Exelis VIS academic programs, using the ENVI Platform, enables labs and classrooms to be outfitted with software and makes software accessible to students. The ENVI software provides students hands on experience with remote sensing software, an easy teaching platform for professors and allows researchers scientifically vetted software they can trust. Training materials are provided at no additional cost and can either serve as a basis for course curriculum development or self paced learning. Non-profit organizations like The Nature Conservancy (TNC) and CGIAR have deployed ENVI and IDL enterprise wide licensing allowing researchers all over the world to have cost effective access COTS software for their research. Exelis VIS has also contributed licenses to the NASA DEVELOP program. Exelis VIS is committed to supporting the academic and NGO community with affordable enterprise licensing, access to training materials, and technical expertise to help researchers tackle today's Earth and Planetary science big data challenges.

Development of a Universal Waste Management System

NASA Technical Reports Server (NTRS)

Baccus, Shelley; Broyan, James L., Jr.

2013-01-01

A concept for a Universal Waste Management System (UWMS) has been developed based on the knowledge gained from over 50 years of space travel. It is being designed for Commercial Orbital Transportation Services (COTS) and Multi ]Purpose Crew Vehicle (MPCV) and is based upon the Extended Duration Orbiter (EDO) commode. The UMWS was modified to enhance crew interface and reduce volume and cost. The UWMS will stow waste in fecal canisters, similar to the EDO, and urine will be stowed in bags for in orbit change out. This allows the pretreated urine to be subsequently processed and recovered as drinking water. The new design combines two fans and a rotary phase separator on a common shaft to allow operation by a single motor. This change enhances packaging by reducing the volume associated with an extra motor, associated controller, harness, and supporting structure. The separator pumps urine to either a dual bag design for COTS vehicles or directly into a water reclamation system. The commode is supported by a concentric frame, enhancing its structural integrity while further reducing the volume from the previous design. The UWMS flight concept development effort is underway and an early output of the development will be a ground based UMWS prototype for manned testing. Referred to as the Gen 3 unit, this prototype will emulate the crew interface included in the UWMS and will offer a great deal of knowledge regarding the usability of the new design, allowing the design team the opportunity to modify the UWMS flight concept based on the manned testing.

Empirical Comparison of Two Psychological Therapies: Self Psychology and Cognitive Orientation in the Treatment of Anorexia and Bulimia

PubMed Central

Bachar, Eytan; Latzer, Yael; Kreitler, Shulamit; Berry, Elliot M.

1999-01-01

The authors investigated the applicability of self psychological treatment (SPT) and cognitive orientation treatment (COT) to the treatment of anorexia and bulimia. Thirty-three patients participated in this study. The bulimic patients (n = 25) were randomly assigned either to SPT, COT, or control/nutritional counseling only (C/NC). The anorexic patients (n = 8) were randomly assigned to either SPT or COT. Patients were administered a battery of outcome measures assessing eating disorders symptomatology, attitudes toward food, self structure, and general psychiatric symptoms. After SPT, significant improvement was observed. After COT, slight but nonsignificant improvement was observed. After C/NC, almost no changes could be detected.(The Journal of Psychotherapy Practice and Research 1999; 8:115–128) PMID:10079459

Mobile, Virtual Enhancements for Rehabilitation (MOVER)

DTIC Science & Technology

2015-05-31

patient uses COTS input devices, such as the Microsoft Kinect and the Wii Balance Board , to perform therapeutic exercises that are mapped to controls...in place of having an exercise creation tool for the therapists, we have simplified the process by hardcoding specific, commonly used balance

Contingency theoretic methodology for agent-based web-oriented manufacturing systems

NASA Astrophysics Data System (ADS)

Durrett, John R.; Burnell, Lisa J.; Priest, John W.

2000-12-01

The development of distributed, agent-based, web-oriented, N-tier Information Systems (IS) must be supported by a design methodology capable of responding to the convergence of shifts in business process design, organizational structure, computing, and telecommunications infrastructures. We introduce a contingency theoretic model for the use of open, ubiquitous software infrastructure in the design of flexible organizational IS. Our basic premise is that developers should change in the way they view the software design process from a view toward the solution of a problem to one of the dynamic creation of teams of software components. We postulate that developing effective, efficient, flexible, component-based distributed software requires reconceptualizing the current development model. The basic concepts of distributed software design are merged with the environment-causes-structure relationship from contingency theory; the task-uncertainty of organizational- information-processing relationships from information processing theory; and the concept of inter-process dependencies from coordination theory. Software processes are considered as employees, groups of processes as software teams, and distributed systems as software organizations. Design techniques already used in the design of flexible business processes and well researched in the domain of the organizational sciences are presented. Guidelines that can be utilized in the creation of component-based distributed software will be discussed.

Cirrus Cloud Optical and Microphysical Property Retrievals from eMAS During SEAC4RS Using Bi-Spectral Reflectance Measurements Within the 1.88 micron Water Vapor Absorption Band

NASA Technical Reports Server (NTRS)

Meyer, K.; Platnick, S.; Arnold, G. T.; Holz, R. E.; Veglio, P.; Yorks, J.; Wang, C.

2016-01-01

Previous bi-spectral imager retrievals of cloud optical thickness (COT) and effective particle radius (CER) based on the Nakajima and King (1990) approach, such as those of the operational MODIS cloud optical property retrieval product (MOD06), have typically paired a non-absorbing visible or near-infrared wavelength, sensitive to COT, with an absorbing shortwave or midwave infrared wavelength sensitive to CER. However, in practice it is only necessary to select two spectral channels that exhibit a strong contrast in cloud particle absorption. Here it is shown, using eMAS observations obtained during NASAs SEAC4RS field campaign, that selecting two absorbing wavelength channels within the broader 1.88 micron water vapor absorption band, namely the 1.83 and 1.93 micron channels that have sufficient differences in ice crystal single scattering albedo, can yield COT and CER retrievals for thin to moderately thick single-layer cirrus that are reasonably consistent with other solar and IR imager-based and lidar-based retrievals. A distinct advantage of this channel selection for cirrus cloud retrievals is that the below cloud water vapor absorption minimizes the surface contribution to measured cloudy TOA reflectance, in particular compared to the solar window channels used in heritage retrievals such as MOD06. This reduces retrieval uncertainty resulting from errors in the surface reflectance assumption, as well as reduces the frequency of retrieval failures for thin cirrus clouds.

Cirrus cloud optical and microphysical property retrievals from eMAS during SEAC4RS using bi-spectral reflectance measurements within the 1.88 µm water vapor absorption band

NASA Astrophysics Data System (ADS)

Meyer, Kerry; Platnick, Steven; Arnold, G. Thomas; Holz, Robert E.; Veglio, Paolo; Yorks, John; Wang, Chenxi

2016-04-01

Previous bi-spectral imager retrievals of cloud optical thickness (COT) and effective particle radius (CER) based on the Nakajima and King (1990) approach, such as those of the operational MODIS cloud optical property retrieval product (MOD06), have typically paired a non-absorbing visible or near-infrared wavelength, sensitive to COT, with an absorbing shortwave or mid-wave infrared wavelength sensitive to CER. However, in practice it is only necessary to select two spectral channels that exhibit a strong contrast in cloud particle absorption. Here it is shown, using eMAS observations obtained during NASA's SEAC4RS field campaign, that selecting two absorbing wavelength channels within the broader 1.88 µm water vapor absorption band, namely the 1.83 and 1.93 µm channels that have sufficient differences in ice crystal single scattering albedo, can yield COT and CER retrievals for thin to moderately thick single-layer cirrus that are reasonably consistent with other solar and IR imager-based and lidar-based retrievals. A distinct advantage of this channel selection for cirrus cloud retrievals is that the below-cloud water vapor absorption minimizes the surface contribution to measured cloudy top-of-atmosphere reflectance, in particular compared to the solar window channels used in heritage retrievals such as MOD06. This reduces retrieval uncertainty resulting from errors in the surface reflectance assumption and reduces the frequency of retrieval failures for thin cirrus clouds.

Sustainment and Net-ready Key Performance Parameters (KPP) in an Enterprise Information System (EIS) Value Assurance Framework (VAF)

DTIC Science & Technology

2014-04-01

adapts the concept of “Communities of Interest” (COI) identified in DoD GIG policy for this purpose. In the VAF construct COIs become hands-on beta...developers. This approach both leverages COTS economy of scale and nudges COTS development in directions useful to the government. Programs can write...schedule  Exploit new GIG acquisition policies  Extend and expand pure COTS competition  Issue simple use cases in lieu of traditional RFI/RFP

Market Research Survey of Commercial Off-The-Shelf (COTS) Portable MS Systems for IAEA Safeguards Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hart, Garret L.; Hager, George J.; Barinaga, Charles J.

2013-02-01

This report summarizes the results for the market research survey of mass spectrometers that are deemed pertinent to International Atomic Energy Agency (IAEA) needs and strategic objectives. The focus of the report is on MS instruments that represent currently available (or soon to be) commercial off-the shelf (COTS) technology and weigh less than 400 pounds. A compilation of all available MS instruments (36 COTS and 2 R&D) is presented, along with pertinent information regarding each instrument.

COTS Initiative Panel Discussion

NASA Image and Video Library

2013-11-13

NASA Administrator Charles Bolden, left, presents NASA's Group Achievement Award to Gwynne Shotwell, President, SpaceX, at NASA Headquarters in Washington on Thursday, November 13, 2013. Shotwell received the award for outstanding contributions and innovative accomplishments in the completion of the Commercial Orbital Transportation Services (COTS) initiative. Through COTS, NASA's partners Space Exploration Technologies Corp. (SpaceX) and Orbital Sciences Corp., developed new U.S. rockets and spacecraft, launched from U.S. soil, capable of transporting cargo to low-Earth orbit and the International Space Station. Photo Credit: (NASA/Jay Westcott)

Preliminary Evaluation Of Commercial Supercapacitors For Space Applications

NASA Astrophysics Data System (ADS)

Gineste, Valery; Loup, Didier; Mattesco, Patrick; Neugnot, Nicolas

2011-10-01

Supercapacitors are identified since years as a new technology enabling energy storage together with high power delivery capability to the system. A recent ESA study [1] led by Astrium has demonstrated the interest of these devices for space application, providing that reliability and end of life performances are demonstrated. A realistic commercial on the shelf (COTS) approach (or with limited design modification approved by potential suppliers) has been favoured (as for batteries). This paper presents preliminary test results done by Astrium on COTS supercapacitors: accelerated life tests, calendar life tests, technology analyses. Based on these results, assessment and lessons learnt are drawn in view of future exhaustive supercapacitor validation and future qualification.

The continent-ocean transition on the northwestern South China Sea

NASA Astrophysics Data System (ADS)

Cameselle, Alejandra L.; Ranero, César R.; Franke, Dieter; Barckhausen, Udo

2015-04-01

Rifted margins are created as a result of stretching and breakup of continental lithosphere that eventually leads to oceanic spreading and formation of a new oceanic basin. A cornerstone for understanding how rift characteristics vary along strike in the same system and what processes control the final transition to seafloor spreading is the continent-ocean transition (COT). We use four regional multichannel seismic profiles and published magnetic lineations to study the structure and variability of COT on the northwest subbasin (NWSB) of the South China Sea and to discern continental from oceanic domains. The continental domain is characterized by tilted fault blocks overlaid by thick syn-rift sedimentary units and fairly continuous Moho reflections typically at 8-10 s twtt. Thickness of the continental crust changes from ~20-25 km under the uppermost slope to ~9-6 km under the lower slope. The oceanic domain is interpreted where a highly reflective top of basement, little faulting, no syntectonic strata, and fairly constant thickness basement (4-8 km) occur. The COT is imaged as a ~5-10 km wide zone where oceanic-type features abut continental-type structures. The South China margin is deformed by abundant normal faults dissecting the continental crust, whereas the conjugate Macclesfield Bank margin displays comparatively abrupt thinning and little faulting. Seismic profiles show an along-strike variation in the tectonic structure of the continental margin. The NE-most lines display ~20-40 km wide segments of intense faulting under the slope and associated continental-crust thinning. Towards the SW, faulting and thinning of the continental crust occurs across a ~100-110 km wide segment. We interpret this 3D structural variability and the narrow COT as a consequence of the abrupt termination of continental rifting tectonics by the NE to SW propagation of a spreading center. We suggest that breakup occurred by spreading center propagation to a larger degree than by lithospheric thinning during continental rifting. Based on the sedimentary successions overlying the oceanic crust, we propose a kinematic evolution for the oceanic domain of the NWSB consisting of a southward spreading center propagation followed by a first narrow ridge jump to the north, and then a younger larger jump to the SW into the east subbasin.

Process Based on SysML for New Launchers System and Software Developments

NASA Astrophysics Data System (ADS)

Hiron, Emmanuel; Miramont, Philippe

2010-08-01

The purpose of this paper is to present the Astrium-ST engineering process based on SysML. This process is currently set-up in the frame of common CNES /Astrium-ST R&T studies related to the Ariane 5 electrical system and flight software modelling. The tool used to set up this process is Rhapsody release 7.3 from IBM-Software firm [1]. This process focuses on the system engineering phase dedicated to Software with the objective to generate both System documents (sequential system design and flight control) and Software specifications.

A Petri Net-Based Software Process Model for Developing Process-Oriented Information Systems

NASA Astrophysics Data System (ADS)

Li, Yu; Oberweis, Andreas

Aiming at increasing flexibility, efficiency, effectiveness, and transparency of information processing and resource deployment in organizations to ensure customer satisfaction and high quality of products and services, process-oriented information systems (POIS) represent a promising realization form of computerized business information systems. Due to the complexity of POIS, explicit and specialized software process models are required to guide POIS development. In this chapter we characterize POIS with an architecture framework and present a Petri net-based software process model tailored for POIS development with consideration of organizational roles. As integrated parts of the software process model, we also introduce XML nets, a variant of high-level Petri nets as basic methodology for business processes modeling, and an XML net-based software toolset providing comprehensive functionalities for POIS development.

A validation study of the simulation software gprMax by varying antenna stand-off height

NASA Astrophysics Data System (ADS)

Wilkinson, Josh; Davidson, Nigel

2018-04-01

The design and subsequent testing of suitable antennas and of complete ground-penetrating radar (GPR) systems can be both time consuming and expensive, with the need to understand the performance of a system in realistic environments of great importance to the end user. Through the use of suitably validated simulations, these costs could be significantly reduced, allowing an economical capability to be built which can accurately predict the performance of novel GPR antennas and existing commercial-off-the-shelf (COTS) systems in a user defined environment. This paper focuses on a preliminary validation of the open source software gprMax1 which features the ability to custom define antennas, targets, clutter objects and realistic heterogeneous soils. As an initial step in the assessment of the software, a comparison of the modelled response of targets buried in sand to experimental data has been undertaken, with the variation in response with antenna stand-off height investigated. This was conducted for both a simple bespoke bow-tie antenna design as well as for a Geophysical Survey Systems, Inc. (GSSI) commercial system,2 building upon previous work3 which explored the fidelity of gprMax in reproducing the S11 of simple antenna designs.

Evaluation of the Trajectory Operations Applications Software Task (TOAST)

NASA Technical Reports Server (NTRS)

Perkins, Sharon; Martin, Andrea; Bavinger, Bill

1990-01-01

The Trajectory Operations Applications Software Task (TOAST) is a software development project under the auspices of the Mission Operations Directorate. Its purpose is to provide trajectory operation pre-mission and real-time support for the Space Shuttle program. As an Application Manager, TOAST provides an isolation layer between the underlying Unix operating system and the series of user programs. It provides two main services: a common interface to operating system functions with semantics appropriate for C or FORTRAN, and a structured input and output package that can be utilized by user application programs. In order to evaluate TOAST as an Application Manager, the task was to assess current and planned capabilities, compare capabilities to functions available in commercially-available off the shelf (COTS) and Flight Analysis Design System (FADS) users for TOAST implementation. As a result of the investigation, it was found that the current version of TOAST is well implemented and meets the needs of the real-time users. The plans for migrating TOAST to the X Window System are essentially sound; the Executive will port with minor changes, while Menu Handler will require a total rewrite. A series of recommendations for future TOAST directions are included.

Comparison of custom versus COTS AMLCDs for military and avionic applications

NASA Astrophysics Data System (ADS)

Angelo, Van

1997-07-01

AMLCD's are currently the flat panel technology of choice for military systems and civil transport avionic applications, both new and retrofit. Historically, military and avionic displays have ben custom designed and have generally been specific to each application. Two recent developments have given display system designers a choice between a custom military/avionic solution or a ruggedized commercial off-the-shelf (COTS) implementation. The first development is the widespread availability of various consumer and automotive AMLCD panels at low prices. The second is the change in the policy of defense departments, notably the US Department of Defense, to procure COTS components instead of developing custom solutions. This paper assesses and analyzes the key differences in characteristics, performance and logistical supportability of military and avionic AMLCD's and presents the tradeoffs involved in making the optimum choice between custom and COTS.

Reliability Assessment for COTS Components in Space Flight Applications

NASA Technical Reports Server (NTRS)

Krishnan, G. S.; Mazzuchi, Thomas A.

2001-01-01

Systems built for space flight applications usually demand very high degree of performance and a very high level of accuracy. Hence, the design engineers are often prone to selecting state-of-art technologies for inclusion in their system design. The shrinking budgets also necessitate use of COTS (Commercial Off-The-Shelf) components, which are construed as being less expensive. The performance and accuracy requirements for space flight applications are much more stringent than those for the commercial applications. The quantity of systems designed and developed for space applications are much lower in number than those produced for the commercial applications. With a given set of requirements, are these COTS components reliable? This paper presents a model for assessing the reliability of COTS components in space applications and the associated affect on the system reliability. We illustrate the method with a real application.

NDS modernization project - requirements analysis report

DOT National Transportation Integrated Search

1997-04-09

The National Distress System (NDS) Modernization Project envisions replacing/modernizing the present VHF-FM based system with an integrated state-of-the-art commercial/government-off- : the-shelf (COTS/GOTS) or non-developmental item (NDI) solution. ...

The experience factory: Can it make you a 5? or what is its relationship to other quality and improvement concepts?

NASA Technical Reports Server (NTRS)

Basili, Victor R.

1992-01-01

The concepts of quality improvements have permeated many businesses. It is clear that the nineties will be the quality era for software and there is a growing need to develop or adapt quality improvement approaches to the software business. Thus we must understand software as an artifact and software as a business. Since the business we are dealing with is software, we must understand the nature of software and software development. The software discipline is evolutionary and experimental; it is a laboratory science. Software is development not production. The technologies of the discipline are human based. There is a lack of models that allow us to reason about the process and the product. All software is not the same; process is a variable, goals are variable, etc. Packaged, reusable, experiences require additional resources in the form of organization, processes, people, etc. There have been a variety of organizational frameworks proposed to improve quality for various businesses. The ones discussed in this presentation include: Plan-Do-Check-Act, a quality improvement process based upon a feedback cycle for optimizing a single process model/production line; the Experience Factory/Quality Improvement Paradigm, continuous improvements through the experimentation, packaging, and reuse of experiences based upon a business's needs; Total Quality Management, a management approach to long term success through customer satisfaction based on the participation of all members of an organization; the SEI capability maturity model, a staged process improvement based upon assessment with regard to a set of key process areas until you reach a level 5 which represents a continuous process improvement; and Lean (software) Development, a principle supporting the concentration of the production on 'value added' activities and the elimination of reduction of 'not value added' activities.

Effects of size, sex, and voluntary running speeds on costs of locomotion in lines of laboratory mice selectively bred for high wheel-running activity.

PubMed

Rezende, Enrico L; Kelly, Scott A; Gomes, Fernando R; Chappell, Mark A; Garland, Theodore

2006-01-01

Selective breeding for over 35 generations has led to four replicate (S) lines of laboratory house mice (Mus domesticus) that run voluntarily on wheels about 170% more than four random-bred control (C) lines. We tested whether S lines have evolved higher running performance by increasing running economy (i.e., decreasing energy spent per unit of distance) as a correlated response to selection, using a recently developed method that allows for nearly continuous measurements of oxygen consumption (VO2) and running speed in freely behaving animals. We estimated slope (incremental cost of transport [COT]) and intercept for regressions of power (the dependent variable, VO2/min) on speed for 49 males and 47 females, as well as their maximum VO2 and speeds during wheel running, under conditions mimicking those that these lines face during the selection protocol. For comparison, we also measured COT and maximum aerobic capacity (VO2max) during forced exercise on a motorized treadmill. As in previous studies, the increased wheel running of S lines was mainly attributable to increased average speed, with males also showing a tendency for increased time spent running. On a whole-animal basis, combined analysis of males and females indicated that COT during voluntary wheel running was significantly lower in the S lines (one-tailed P=0.015). However, mice from S lines are significantly smaller and attain higher maximum speeds on the wheels; with either body mass or maximum speed (or both) entered as a covariate, the statistical significance of the difference in COT is lost (one-tailed P> or =0.2). Thus, both body size and behavior are key components of the reduction in COT. Several statistically significant sex differences were observed, including lower COT and higher resting metabolic rate in females. In addition, maximum voluntary running speeds were negatively correlated with COT in females but not in males. Moreover, males (but not females) from the S lines exhibited significantly higher treadmill VO2max as compared to those from C lines. The sex-specific responses to selection may in part be consequences of sex differences in body mass and running style. Our results highlight how differences in size and running speed can account for lower COT in S lines and suggest that lower COT may have coadapted in response to selection for higher running distances in these lines.

Autonomous, agile micro-satellites and supporting technologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Breitfeller, E; Dittman, M D; Gaughan, R J

1999-07-19

This paper updates the on-going effort at Lawrence Livermore National Laboratory to develop autonomous, agile micro-satellites (MicroSats). The objective of this development effort is to develop MicroSats weighing only a few tens of kilograms, that are able to autonomously perform precision maneuvers and can be used telerobotically in a variety of mission modes. The required capabilities include satellite rendezvous, inspection, proximity-operations, docking, and servicing. The MicroSat carries an integrated proximity-operations sensor-suite incorporating advanced avionics. A new self-pressurizing propulsion system utilizing a miniaturized pump and non-toxic mono-propellant hydrogen peroxide was successfully tested. This system can provide a nominal 25 kg MicroSatmore » with 200-300 m/s delta-v including a warm-gas attitude control system. The avionics is based on the latest PowerPC processor using a CompactPCI bus architecture, which is modular, high-performance and processor-independent. This leverages commercial-off-the-shelf (COTS) technologies and minimizes the effects of future changes in processors. The MicroSat software development environment uses the Vx-Works real-time operating system (RTOS) that provides a rapid development environment for integration of new software modules, allowing early integration and test. We will summarize results of recent integrated ground flight testing of our latest non-toxic pumped propulsion MicroSat testbed vehicle operated on our unique dynamic air-rail.« less

IQ-Station: A Low Cost Portable Immersive Environment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eric Whiting; Patrick O'Leary; William Sherman

2010-11-01

The emergence of inexpensive 3D TV’s, affordable input and rendering hardware and open-source software has created a yeasty atmosphere for the development of low-cost immersive environments (IE). A low cost IE system, or IQ-station, fashioned from commercial off the shelf technology (COTS), coupled with a targeted immersive application can be a viable laboratory instrument for enhancing scientific workflow for exploration and analysis. The use of an IQ-station in a laboratory setting also has the potential of quickening the adoption of a more sophisticated immersive environment as a critical enabler in modern scientific and engineering workflows. Prior work in immersive environmentsmore » generally required either a head mounted display (HMD) system or a large projector-based implementation both of which have limitations in terms of cost, usability, or space requirements. The solution presented here provides an alternative platform providing a reasonable immersive experience that addresses those limitations. Our work brings together the needed hardware and software to create a fully integrated immersive display and interface system that can be readily deployed in laboratories and common workspaces. By doing so, it is now feasible for immersive technologies to be included in researchers’ day-to-day workflows. The IQ-Station sets the stage for much wider adoption of immersive environments outside the small communities of virtual reality centers.« less

Evaluation of low-cost commercial-off-the-shelf autopilot systems for SUAS operations

NASA Astrophysics Data System (ADS)

Brown, Calvin Thomas

With this increase in unmanned aircraft system (UAS) operations, there is a need for a structured process to evaluate different commercially available systems, particularly autopilots. The Remotely Operated Aircraft Management, Interpretation, and Navigation from Ground or ROAMING scale was developed to meet this need. This scale is a modification of the widely accepted Handling Qualities Rating scale developed by George Cooper and Robert Harper Jr. The Cooper-Harper scale allows pilots to rate a vehicle's performance in completing some task. Similarly, the ROAMING scale allows UAS operators to evaluate the management and observability of UAS in completing some task. The standardized evaluative process consists of cost, size, weight, and power (SWAP) analysis, ease of implementation through procedural description of setup, ROAMING scale rating, a slightly modified NASA TLX rating, and comparison of manual operation to autonomous operation of the task. This standard for evaluation of autopilots and their software will lead to better understanding of the workload placed on UAS operators and indicate where improvements to design and operational procedures can be made. An assortment of low-cost commercial-off-the-shelf (COTS) autopilots were selected for use in the development of the evaluation and results of these tests demonstrate the commonalities and differences in these systems.

A Cost Effective System Design Approach for Critical Space Systems

NASA Technical Reports Server (NTRS)

Abbott, Larry Wayne; Cox, Gary; Nguyen, Hai

2000-01-01

NASA-JSC required an avionics platform capable of serving a wide range of applications in a cost-effective manner. In part, making the avionics platform cost effective means adhering to open standards and supporting the integration of COTS products with custom products. Inherently, operation in space requires low power, mass, and volume while retaining high performance, reconfigurability, scalability, and upgradability. The Universal Mini-Controller project is based on a modified PC/104-Plus architecture while maintaining full compatibility with standard COTS PC/104 products. The architecture consists of a library of building block modules, which can be mixed and matched to meet a specific application. A set of NASA developed core building blocks, processor card, analog input/output card, and a Mil-Std-1553 card, have been constructed to meet critical functions and unique interfaces. The design for the processor card is based on the PowerPC architecture. This architecture provides an excellent balance between power consumption and performance, and has an upgrade path to the forthcoming radiation hardened PowerPC processor. The processor card, which makes extensive use of surface mount technology, has a 166 MHz PowerPC 603e processor, 32 Mbytes of error detected and corrected RAM, 8 Mbytes of Flash, and I Mbytes of EPROM, on a single PC/104-Plus card. Similar densities have been achieved with the quad channel Mil-Std-1553 card and the analog input/output cards. The power management built into the processor and its peripheral chip allows the power and performance of the system to be adjusted to meet the requirements of the application, allowing another dimension to the flexibility of the Universal Mini-Controller. Unique mechanical packaging allows the Universal Mini-Controller to accommodate standard COTS and custom oversized PC/104-Plus cards. This mechanical packaging also provides thermal management via conductive cooling of COTS boards, which are typically designed for convection cooling methods.

Session on High Speed Civil Transport Design Capability Using MDO and High Performance Computing

NASA Technical Reports Server (NTRS)

Rehder, Joe

2000-01-01

Since the inception of CAS in 1992, NASA Langley has been conducting research into applying multidisciplinary optimization (MDO) and high performance computing toward reducing aircraft design cycle time. The focus of this research has been the development of a series of computational frameworks and associated applications that increased in capability, complexity, and performance over time. The culmination of this effort is an automated high-fidelity analysis capability for a high speed civil transport (HSCT) vehicle installed on a network of heterogeneous computers with a computational framework built using Common Object Request Broker Architecture (CORBA) and Java. The main focus of the research in the early years was the development of the Framework for Interdisciplinary Design Optimization (FIDO) and associated HSCT applications. While the FIDO effort was eventually halted, work continued on HSCT applications of ever increasing complexity. The current application, HSCT4.0, employs high fidelity CFD and FEM analysis codes. For each analysis cycle, the vehicle geometry and computational grids are updated using new values for design variables. Processes for aeroelastic trim, loads convergence, displacement transfer, stress and buckling, and performance have been developed. In all, a total of 70 processes are integrated in the analysis framework. Many of the key processes include automatic differentiation capabilities to provide sensitivity information that can be used in optimization. A software engineering process was developed to manage this large project. Defining the interactions among 70 processes turned out to be an enormous, but essential, task. A formal requirements document was prepared that defined data flow among processes and subprocesses. A design document was then developed that translated the requirements into actual software design. A validation program was defined and implemented to ensure that codes integrated into the framework produced the same results as their standalone counterparts. Finally, a Commercial Off the Shelf (COTS) configuration management system was used to organize the software development. A computational environment, CJOPT, based on the Common Object Request Broker Architecture, CORBA, and the Java programming language has been developed as a framework for multidisciplinary analysis and Optimization. The environment exploits the parallelisms inherent in the application and distributes the constituent disciplines on machines best suited to their needs. In CJOpt, a discipline code is "wrapped" as an object. An interface to the object identifies the functionality (services) provided by the discipline, defined in Interface Definition Language (IDL) and implemented using Java. The results of using the HSCT4.0 capability are described. A summary of lessons learned is also presented. The use of some of the processes, codes, and techniques by industry are highlighted. The application of the methodology developed in this research to other aircraft are described. Finally, we show how the experience gained is being applied to entirely new vehicles, such as the Reusable Space Transportation System. Additional information is contained in the original.

Photogrammetry Tool for Forensic Analysis

NASA Technical Reports Server (NTRS)

Lane, John

2012-01-01

A system allows crime scene and accident scene investigators the ability to acquire visual scene data using cameras for processing at a later time. This system uses a COTS digital camera, a photogrammetry calibration cube, and 3D photogrammetry processing software. In a previous instrument developed by NASA, the laser scaling device made use of parallel laser beams to provide a photogrammetry solution in 2D. This device and associated software work well under certain conditions. In order to make use of a full 3D photogrammetry system, a different approach was needed. When using multiple cubes, whose locations relative to each other are unknown, a procedure that would merge the data from each cube would be as follows: 1. One marks a reference point on cube 1, then marks points on cube 2 as unknowns. This locates cube 2 in cube 1 s coordinate system. 2. One marks reference points on cube 2, then marks points on cube 1 as unknowns. This locates cube 1 in cube 2 s coordinate system. 3. This procedure is continued for all combinations of cubes. 4. The coordinate of all of the found coordinate systems is then merged into a single global coordinate system. In order to achieve maximum accuracy, measurements are done in one of two ways, depending on scale: when measuring the size of objects, the coordinate system corresponding to the nearest cube is used, or when measuring the location of objects relative to a global coordinate system, a merged coordinate system is used. Presently, traffic accident analysis is time-consuming and not very accurate. Using cubes with differential GPS would give absolute positions of cubes in the accident area, so that individual cubes would provide local photogrammetry calibration to objects near a cube.

A Shuttle Story: Latchup Qualification of a Truly Commercial Instrument

NASA Technical Reports Server (NTRS)

Swift, G.; Edmonds, L.; O'Conner, M.; Smith, L.; Tubbs, E.

1998-01-01

Destructive single event effects (DSEE) are a main concern for pioneers attempting to use COTS (commercial-off-the-shelf) devices in space, so a COTS instrument full of such devices is particulary scary.

EPA iComplaints

EPA Pesticide Factsheets

The iComplaints system is an enterprise-level COTS (Commercial Off-The-Shelf) product that provides all of the funtionality required to collect, track, manage, process and report on information regarding internal EEO complaints in accordance with several civil rights laws and regulations, to include but not limited to, Title VII of the Civil Rights Act.

KSC-2012-2511

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians load cargo into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2513

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians load cargo into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2512

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians load cargo into the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2510

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, preparations are under way to load cargo into the Space Exploration Technologies Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2514

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, Space Exploration Technologies technicians stow cargo in the Dragon capsule in preparation for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann

KSC-2012-2516

NASA Image and Video Library

2012-04-04

CAPE CANAVERAL, Fla. – In a processing hangar at Space Launch Complex-40 on Cape Canaveral Air Force Station in Florida, a cargo bag slides through the docking ring into the Space Exploration Technologies Dragon capsule for stowage for its scheduled April 30 liftoff aboard a Falcon 9 rocket. Known as SpaceX, the launch will be the company's second demonstration test flight for NASA's Commercial Orbital Transportation Services program, or COTS. During the flight, the capsule will conduct a series of checkout procedures to test and prove its systems, including rendezvous and berthing with the International Space Station. The cargo includes food and provisions for the station’s Expedition crews, such as clothing, batteries, and computer equipment. Under COTS, NASA has partnered with two private companies to launch cargo safely to the station. For more information, visit http://www.nasa.gov/spacex. Photo credit: NASA/Jim Grossmann