45 CFR 1309.52 - Procurement procedures.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF HEALTH AND HUMAN SERVICES THE ADMINISTRATION FOR CHILDREN, YOUTH AND FAMILIES, HEAD START PROGRAM HEAD START FACILITIES PURCHASE, MAJOR RENOVATION AND CONSTRUCTION Construction and Major Renovation § 1309.52 Procurement procedures. (a) All facility construction and major renovation transactions...

13 CFR 305.6 - Allowable methods of procurement for construction services.

Code of Federal Regulations, 2013 CFR

2013-01-01

... design/build, construction management at risk and force account. If an alternate method is used, the... for construction services. 305.6 Section 305.6 Business Credit and Assistance ECONOMIC DEVELOPMENT... Approved Projects § 305.6 Allowable methods of procurement for construction services. (a) Recipients may...

13 CFR 305.6 - Allowable methods of procurement for construction services.

Code of Federal Regulations, 2012 CFR

2012-01-01

... design/build, construction management at risk and force account. If an alternate method is used, the... for construction services. 305.6 Section 305.6 Business Credit and Assistance ECONOMIC DEVELOPMENT... Approved Projects § 305.6 Allowable methods of procurement for construction services. (a) Recipients may...

13 CFR 305.6 - Allowable methods of procurement for construction services.

Code of Federal Regulations, 2014 CFR

2014-01-01

... design/build, construction management at risk and force account. If an alternate method is used, the... for construction services. 305.6 Section 305.6 Business Credit and Assistance ECONOMIC DEVELOPMENT... Approved Projects § 305.6 Allowable methods of procurement for construction services. (a) Recipients may...

7 CFR 1780.72 - Procurement methods.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 12 2012-01-01 2012-01-01 false Procurement methods. 1780.72 Section 1780.72..., Constructing and Inspections § 1780.72 Procurement methods. Procurement shall be made by one of the following methods: Small purchase procedures; competitive sealed bids (formal advertising); competitive negotiation...

7 CFR 1780.72 - Procurement methods.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 12 2013-01-01 2013-01-01 false Procurement methods. 1780.72 Section 1780.72..., Constructing and Inspections § 1780.72 Procurement methods. Procurement shall be made by one of the following methods: Small purchase procedures; competitive sealed bids (formal advertising); competitive negotiation...

7 CFR 1780.72 - Procurement methods.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 12 2014-01-01 2013-01-01 true Procurement methods. 1780.72 Section 1780.72..., Constructing and Inspections § 1780.72 Procurement methods. Procurement shall be made by one of the following methods: Small purchase procedures; competitive sealed bids (formal advertising); competitive negotiation...

7 CFR 1780.72 - Procurement methods.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 12 2010-01-01 2010-01-01 false Procurement methods. 1780.72 Section 1780.72..., Constructing and Inspections § 1780.72 Procurement methods. Procurement shall be made by one of the following methods: Small purchase procedures; competitive sealed bids (formal advertising); competitive negotiation...

7 CFR 1780.72 - Procurement methods.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 12 2011-01-01 2011-01-01 false Procurement methods. 1780.72 Section 1780.72..., Constructing and Inspections § 1780.72 Procurement methods. Procurement shall be made by one of the following methods: Small purchase procedures; competitive sealed bids (formal advertising); competitive negotiation...

14 CFR Appendix C to Part 152 - Procurement Procedures and Requirements

Code of Federal Regulations, 2014 CFR

2014-01-01

.... The sponsor shall establish procedures for procurement of supplies, equipment, construction, and... other State, possession, or territory of the United States in the construction of a project. 3. Bid guarantee. All bids for construction or facility improvement in excess of $100,000 shall be accompanied by a...

14 CFR Appendix C to Part 152 - Procurement Procedures and Requirements

Code of Federal Regulations, 2010 CFR

2010-01-01

.... The sponsor shall establish procedures for procurement of supplies, equipment, construction, and... other State, possession, or territory of the United States in the construction of a project. 3. Bid guarantee. All bids for construction or facility improvement in excess of $100,000 shall be accompanied by a...

14 CFR Appendix C to Part 152 - Procurement Procedures and Requirements

Code of Federal Regulations, 2012 CFR

2012-01-01

.... The sponsor shall establish procedures for procurement of supplies, equipment, construction, and... other State, possession, or territory of the United States in the construction of a project. 3. Bid guarantee. All bids for construction or facility improvement in excess of $100,000 shall be accompanied by a...

14 CFR Appendix C to Part 152 - Procurement Procedures and Requirements

Code of Federal Regulations, 2013 CFR

2013-01-01

.... The sponsor shall establish procedures for procurement of supplies, equipment, construction, and... other State, possession, or territory of the United States in the construction of a project. 3. Bid guarantee. All bids for construction or facility improvement in excess of $100,000 shall be accompanied by a...

14 CFR Appendix C to Part 152 - Procurement Procedures and Requirements

Code of Federal Regulations, 2011 CFR

2011-01-01

.... The sponsor shall establish procedures for procurement of supplies, equipment, construction, and... other State, possession, or territory of the United States in the construction of a project. 3. Bid guarantee. All bids for construction or facility improvement in excess of $100,000 shall be accompanied by a...

Addendum to the Final Audit Report on Procurement of Spare Parts and Supplies

DTIC Science & Technology

1993-06-04

Contraves USA, Simulation and Systems Integration, Tampa, FL Contract No.: N00104-89-G-A050-4009 Awarded: April 20, 1990 Procurement Method...awarded sole source to Contraves . The amplifier is a source-controlled item, and Contraves is the only approved source. There are no commercial...The total contract value was $69,552. Contraves assembled and tested the amplifiers. For the subject procurement, Contraves paid $* per unit for the

Green related practices for construction procurement

NASA Astrophysics Data System (ADS)

Bidin, Z. A.; Bohari, A. A. M.; Rais, S. L. A.; Saferi, M. M.

2018-04-01

Environment degradation issue has been a worldwide problem and various efforts have been done to minimise the problem. Construction sectors are pointed as one of the major contributors to its construction activities. Green-oriented procurement [GP] is considered as an environmental strategy to integrate the environmental practices into the construction delivery. Thus, this paper reviews the concept of GP and the practices related to it. The outcome of this research findings provides a basis for understanding the concept of GP and the further development of GP. The listed GP practices identified from this paper can serve as guidelines for industry practitioners to design, implement, and benchmarking the green practices in their procurement delivery.

7 CFR 3565.257 - Procurement actions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 15 2011-01-01 2011-01-01 false Procurement actions. 3565.257 Section 3565.257... AGRICULTURE GUARANTEED RURAL RENTAL HOUSING PROGRAM Property Requirements § 3565.257 Procurement actions. All construction procurement actions, whether by sealed bid or by negotiation, must be conducted in a manner that...

7 CFR 3565.257 - Procurement actions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Procurement actions. 3565.257 Section 3565.257... AGRICULTURE GUARANTEED RURAL RENTAL HOUSING PROGRAM Property Requirements § 3565.257 Procurement actions. All construction procurement actions, whether by sealed bid or by negotiation, must be conducted in a manner that...

48 CFR 52.223-2 - Affirmative Procurement of Biobased Products Under Service and Construction Contracts.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Biobased Products Under Service and Construction Contracts. 52.223-2 Section 52.223-2 Federal Acquisition... CONTRACT CLAUSES Text of Provisions and Clauses 52.223-2 Affirmative Procurement of Biobased Products Under Service and Construction Contracts. As prescribed in 23.406(b), insert the following clause: Affirmative...

Present status and future prospects of the JT-60SA project

NASA Astrophysics Data System (ADS)

Ishida, S.; Barabaschi, P.; Kamada, Y.

2014-10-01

The JT-60SA project has been implemented jointly by Europe and Japan since June 2007. After the disassembly of JT-60 from the torus hall had been completed in October 2012, the project achieved the major milestone of starting the tokamak's assembly at the JAEA Naka site in January 2013 following the completion of the cryostat base in Europe and its transport to Japan. Procurement and assembly activities for components such as the superconducting magnet, cryogenic system, power supply, vacuum vessel, divertor and cryostat are progressing on track towards the start of operation in March 2019. In preparation for exploitation, the JT-60SA Research Plan was issued in December 2011, and the research integration activities are addressing JT-60SA data management, validation and analysis tools. This paper overviews the latest evolution of the project in terms of construction and exploitation for JT-60SA.

A STUDY ON IMPLEMENTATION OF PROCUREMENT MANAGEMENT METHOD THAT DELIVERLY'S UNCERTAINTY OF EQUIPMENTS, MATERIALS AND INSTRUCTIONS

NASA Astrophysics Data System (ADS)

Asaine, Keita; Asaine, Wataru; Shiratsuchi, Ryoma; Yoshida, Takaichi; Hashimoto, Masaaki

This paper highlights the issues of procurement management in construction projects, such as late delivery of purchased equipments/materials and missing instructions from customers, which cause delays of construction schedule and over-budget cost. We point that most of these problems are caused by lack of synchronization between procurement activities and process control. Therefore, we propose a managerial method which enables better synchronization between the two by applying this method to a construction company. We discuss the necessary conditions and validity of incorporating it and show the way how to establish the mechanics through the case study. Furthermore, we analyze that the feature of this method is not only addressing procurement issues but also bringing additional benefits, such as shortening project lead time and reducing project cost.

75 FR 35123 - Notice of Buy America Waiver for Minivans and Minivan Chassis

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-21

... change its final assembly location from the United States to Japan; and Toyota has not responded to public procurements.\\1\\ \\1\\ Contrary to Chrysler's assertion that Toyota has not responded to public procurements, in May 2010 FTA learned that Toyota may have certified compliance with the Buy America...

13 CFR 125.2 - Prime contracting assistance.

Code of Federal Regulations, 2013 CFR

2013-01-01

... participation unlikely; (ii) Seeks to package or consolidate discrete construction projects; or (iii) Meets the... participation unlikely, or if a proposed procurement for construction seeks to package or consolidate discrete... discrete projects. (5) In conjunction with their duties to promote the set-aside of procurements for small...

13 CFR 125.2 - Prime contracting assistance.

Code of Federal Regulations, 2012 CFR

2012-01-01

... participation unlikely; (ii) Seeks to package or consolidate discrete construction projects; or (iii) Meets the... participation unlikely, or if a proposed procurement for construction seeks to package or consolidate discrete... discrete projects. (5) In conjunction with their duties to promote the set-aside of procurements for small...

13 CFR 125.2 - Prime contracting assistance.

Code of Federal Regulations, 2011 CFR

2011-01-01

... participation unlikely; (ii) Seeks to package or consolidate discrete construction projects; or (iii) Meets the... participation unlikely, or if a proposed procurement for construction seeks to package or consolidate discrete... discrete projects. (5) In conjunction with their duties to promote the set-aside of procurements for small...

41 CFR 61-300.1 - What are the purpose and scope of this part?

Code of Federal Regulations, 2011 CFR

2011-07-01

... States for the procurement of personal property and non-personal services (including construction), and... for the procurement of personal property and non-personal services (including construction) was... Relating to Public Contracts OFFICE OF THE ASSISTANT SECRETARY FOR VETERANS' EMPLOYMENT AND TRAINING...

41 CFR 61-300.1 - What are the purpose and scope of this part?

Code of Federal Regulations, 2014 CFR

2014-07-01

... States for the procurement of personal property and non-personal services (including construction), and... for the procurement of personal property and non-personal services (including construction) was... Relating to Public Contracts OFFICE OF THE ASSISTANT SECRETARY FOR VETERANS' EMPLOYMENT AND TRAINING...

41 CFR 61-300.1 - What are the purpose and scope of this part?

Code of Federal Regulations, 2012 CFR

2012-07-01

... States for the procurement of personal property and non-personal services (including construction), and... for the procurement of personal property and non-personal services (including construction) was... Relating to Public Contracts OFFICE OF THE ASSISTANT SECRETARY FOR VETERANS' EMPLOYMENT AND TRAINING...

41 CFR 61-300.1 - What are the purpose and scope of this part?

Code of Federal Regulations, 2010 CFR

2010-07-01

... States for the procurement of personal property and non-personal services (including construction), and... for the procurement of personal property and non-personal services (including construction) was... Relating to Public Contracts OFFICE OF THE ASSISTANT SECRETARY FOR VETERANS' EMPLOYMENT AND TRAINING...

41 CFR 61-300.1 - What are the purpose and scope of this part?

Code of Federal Regulations, 2013 CFR

2013-07-01

... States for the procurement of personal property and non-personal services (including construction), and... for the procurement of personal property and non-personal services (including construction) was... Relating to Public Contracts OFFICE OF THE ASSISTANT SECRETARY FOR VETERANS' EMPLOYMENT AND TRAINING...

10 CFR 52.99 - Inspection during construction.

Code of Federal Regulations, 2011 CFR

2011-01-01

... license may proceed at its own risk with design and procurement activities, and a licensee may proceed at its own risk with design, procurement, construction, and pre-operational activities, even though the... 10 CFR 50.10(a), whichever is later, its schedule for completing the inspections, tests, or analyses...

10 CFR 52.99 - Inspection during construction.

Code of Federal Regulations, 2012 CFR

2012-01-01

... license may proceed at its own risk with design and procurement activities, and a licensee may proceed at its own risk with design, procurement, construction, and pre-operational activities, even though the... 10 CFR 50.10(a), whichever is later, its schedule for completing the inspections, tests, or analyses...

10 CFR 52.99 - Inspection during construction.

Code of Federal Regulations, 2010 CFR

2010-01-01

... license may proceed at its own risk with design and procurement activities, and a licensee may proceed at its own risk with design, procurement, construction, and pre-operational activities, even though the... 10 CFR 50.10(a), whichever is later, its schedule for completing the inspections, tests, or analyses...

Production of Copper-Plated Beamline Bellows and Spools for LCLS-II

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, Katherine M.; Carpenter, Brian C.; Daly, Ed

The SLAC National Accelerator Laboratory is currently constructing a major upgrade to its accelerator, the Linac Coherent Light Source II (LCLS-II). Several Department of Energy national laboratories, including the Thomas Jefferson National Accelerator Facility (JLab) and Fermi National Accelerator Laboratory (FNAL), are participating in this project. The 1.3-GHz cryomodules for this project consist of eight cavities separated by bellows (expansion joints) and spools (tube sections), which are copper plated for RF conduction. JLab is responsible for procurement of these bellows and spools, which are delivered to JLab and FNAL for assembly into cryomodules. Achieving accelerator-grade copper plating is always amore » challenge and requires careful specification of requirements and application of quality control processes. Due to the demanding technical requirements of this part, JLab implemented procurement strategies to make the process more efficient as well as provide process redundancy. This paper discusses the manufacturing challenges that were encountered and resolved, as well as the strategies that were employed to minimize the impact of any technical issues.« less

13 CFR 124.507 - What procedures apply to competitive 8(a) procurements?

Code of Federal Regulations, 2010 CFR

2010-01-01

... boundaries are eligible to submit offers. (3) Competition for all non-construction requirements. Except for... procedures. Procuring activities will conduct competitions among and evaluate offers received from... the Participant submitted its initial offer which includes price. (5) If the procuring activity...

Cell module and fuel conditioner

NASA Technical Reports Server (NTRS)

Hoover, D. Q., Jr.

1980-01-01

Measurements of stack height changes with temperature and cell material characteristics were made. Stack 559 was assembled and components were fabricated for 560, 561, and 562. Stack 425 was transferred from the parallel DOE program and installed in the OS/IES simulation loop for mechanical and electrical testing. Construction and preliminary checkout of the 2 kW test facility was completed and design and procurement of the 8 kW test facility was initiated. The fuel conditioning subsystem design continued to evolve and the state points for the current design were calculated at full and part load conditions. Steam reforming catalyst activity tests were essentially completed and aging tests and CO shift converter tests were initiated.

NASA procurement report, FY 1992

NASA Technical Reports Server (NTRS)

1992-01-01

This report presents summary data on all NASA procurement actions and detailed information on contracts, grants, agreements, and other procurements over $25,000 awarded by NASA during the first six months of Fiscal Year 1992. The dollar value on procurements over $25,000 amounted to 98 percent of the total dollar value of procurement actions completed during the first six months of Fiscal Year 1992. However, these larger procurements accounted for only 29 percent of the total actions. Procurement action, as used in this report, means contractual actions to obtain supplies, services, or construction which increase or decrease funds. A procurement action thus may be a new procurement, or modifications such as supplementary agreements, change orders, or terminations to a contract that change the total amount of funds obligated.

Evaluation of Ammunition Data Cards (REDACTED)

DTIC Science & Technology

2016-04-29

Procuring Contracting Officer ( PCO ), the manufacturer shall include, in the components sections on ADC representing the munition, all assemblies...lot components or evidence of PCO waiver approval. As a result, ADCs did not consistently include component items that make up the ammunition, and...Energetics PCO Procuring Contract Officer PQM Product Quality Manager QALI Quality Assurance Letter of Instruction QAR Quality Assurance Representative

40 CFR 31.36 - Procurement.

Code of Federal Regulations, 2013 CFR

2013-07-01

... procurements, contain features which unduly restrict competition. The description may include a statement of... requirements of a procurement. The specific features of the named brand which must be met by offerors shall be...) Construction grants awarded under Title II of the Clean Water Act are subject to the following “Buy American...

40 CFR 31.36 - Procurement.

Code of Federal Regulations, 2012 CFR

2012-07-01

... procurements, contain features which unduly restrict competition. The description may include a statement of... requirements of a procurement. The specific features of the named brand which must be met by offerors shall be...) Construction grants awarded under Title II of the Clean Water Act are subject to the following “Buy American...

40 CFR 31.36 - Procurement.

Code of Federal Regulations, 2014 CFR

2014-07-01

... procurements, contain features which unduly restrict competition. The description may include a statement of... requirements of a procurement. The specific features of the named brand which must be met by offerors shall be...) Construction grants awarded under Title II of the Clean Water Act are subject to the following “Buy American...

40 CFR 35.936-21 - Delegation to State agencies; certification of procurement systems.

Code of Federal Regulations, 2010 CFR

2010-07-01

...; certification of procurement systems. 35.936-21 Section 35.936-21 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.936-21 Delegation to State agencies; certification of procurement...

NASA procurement report, FY 1993

NASA Technical Reports Server (NTRS)

1993-01-01

Summary data on all NASA procurement actions and detailed information on contracts, grants, agreements, and other procurements over $25,000 awarded by NASA during fiscal year 1993. The dollar value on procurements over $25,000 amounted to 97 percent of the total dollar value of procurement actions completed during fiscal year 1993. However, these larger procurements accounted for only 29 percent of the total actions. Procurement action, as used in this report, means contractural actions to obtain supplies, services, or construction which increase or decrease funds. A procurement action thus may be a new procurement, or modifications such as supplemental agreements, change orders, or terminations to an existing contract that change the total amount of funds obligated. An obligation is a contractual commitment to pay for supplies or services that are specified in the contract.

Annual Procurement Report, Fiscal Year 1997

NASA Technical Reports Server (NTRS)

1997-01-01

This report presents summary data on all NASA procurement actions and detailed information on contracts, grants, agreements, and other procurements over $25,000 awarded by NASA during Fiscal Year 1997. The dollar value on procurements over $25,000 amounted to 98 percent of the total dollar value of procurement actions completed during Fiscal Year 1997. However, these larger procurements accounted for only 43 percent of the total actions. Procurement action, as used in this report means contractual actions to obtain supplies, services or construction which increase or decrease funds. A procurement action thus may be a new procurement or modifications such as supplemental agreements, change orders, or termination's to an existing contract that change the total amount of funds obligated. An obligation is a contractual commitment to pay for supplies or services that are specified in the contract. The Annual Procurement Report is also available via the internet on the Procurement homepage.

Development of standardized specifications for silicon solar cells

NASA Technical Reports Server (NTRS)

Scott-Monck, J. A.

1977-01-01

A space silicon solar cell assembly (cell and coverglass) specification aimed at standardizing the diverse requirements of current cell or assembly specifications was developed. This specification was designed to minimize both the procurement and manufacturing costs for space qualified silicon solar cell assembilies. In addition, an impact analysis estimating the technological and economic effects of employing a standardized space silicon solar cell assembly was performed.

Quality assurance program guidelines for application to and use by manufacturers of rail/guideway vehicles, buses, automatic train control systems, and their major subsystems

NASA Technical Reports Server (NTRS)

Witkin, S. A.

1976-01-01

Guidelines are presented for a quality assurance system to be implemented by the manufacturer in support of designing, developing, fabricating, assembling, inspecting, testing, handling, and delivery of equipment being procured for use in public urban mass transit systems. The guidelines apply to this equipment when being procured for: (1) use in revenue service; (2) demonstration of systems that will be revenue producing or used by the public; (3) use as a prototype for follow-on operational/revenue producing equipment procurements; and (4) qualification tests.

Summary data on all NASA procurement actions

NASA Technical Reports Server (NTRS)

1992-01-01

This report presents summary data on all NASA procurement actions and detailed information on contracts, grants, agreements, and other procurements over $25,000 awarded by NASA during Fiscal Year 1992 (FY-92). The dollar value on procurements over $25,000 amounted to 97 percent of the total dollar value of procurement actions completed during FY-92. However, these larger procurements accounted for only 28 percent of the total actions. Procurement action, as used in this report, means contractual actions to obtain supplies, services, or construction which increase or decrease funds. A procurement action thus may be a new procurement or modifications such as supplemental agreements, change orders, or terminations to an existing contract that change the total amount of funds obligated. An obligation is a contractual commitment to pay for supplies or services that are specified in the contract.

Energy Systems Integration Newsletter | Energy Systems Integration Facility

Science.gov Websites

simulated sequences based on a model network. The competitive procurement process provided comparative , procurement help, design reviews, and now construction support. Miramar project support is part of integrated

ITS procurement : analysis and recommendations.

DOT National Transportation Integrated Search

1994-01-01

Virginia has met with a variety of difficulties and delays in its procurement of intelligent transportation systems, components, and studies. The Fastoll Automatic Toll System was initiated in 1987, and construction has still not begun. It has been t...

Long-Term Implications of the 2016 Future Years Defense Program

DTIC Science & Technology

2016-01-01

operation and maintenance activities; acquisition includes research, development, test, and evaluation as well as procurement of weapon systems and other...group includes procurement and research, development, test, and evaluation (RDT&E). Appropriations for procurement fund the purchase of new weapon...and Evaluation Military Construction Family Housing OCO Funding Actual FYDP Period Beyond the FYDP PeriodDoD’s Estimates For 2017 through 2020 DoD

76 FR 81940 - Submission for OMB Review; Biobased Procurements

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-29

... DEPARTMENT OF DEFENSE GENERAL SERVICES ADMINISTRATION NATIONAL AERONAUTICS AND SPACE... Procurements AGENCY: Department of Defense (DOD), General Services Administration (GSA), and National... Products Under Service and Construction Contracts, is being revised to require prime contractors to report...

78 FR 71581 - Procurement List; Additions and Deletions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-29

... Prime Vendor Supply Chain Management Service [to support production, assembly, receipt, storage... Army, W6QK ACC-APG Natick, Natick, MA. Service Type/Location: Integrated Prime Vendor, Supply Chain...

OAS :: Accountability :: Procurement

Science.gov Websites

Disarmament Drugs E e-Government Education Elections Environment Equity G General Assembly Governance H Human Management Public Security R Racism and Intolerance Refugees S Scholarships School of Governance Science and

Procurement of novel microanalysis equipment for construction materials.

DOT National Transportation Integrated Search

2012-02-01

The equipment procured (i.e. an Orbis micro X-ray Fluorescence (MXRF) and an APSEX personal Scanning Electron Microscope (PSEM)) is part of the next generation of micro analytical equipment. These tools have the ability to make large volumes of...

78 FR 41694 - Final Priority and Requirements; Education Facilities Clearinghouse

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-11

... the collection and dissemination of best practices for the planning, design, financing, procurement... Facilities Clearinghouse program is to provide technical assistance and training on the planning, design... dissemination of best practices for the planning, design, financing, procurement, construction, improvement...

Procurement of construction services: a case study on bidding competition in Singapore public sector contracts

NASA Astrophysics Data System (ADS)

Oo, B. L.; Yan, Y.

2018-04-01

There are many variables that public clients need to consider in their bidding procedure to enhance efficiency in their procurement for construction services. This paper focusses on the competitive bidding process for public sector procurement of construction services in Singapore. A distinctive feature of the Singapore government competitive bidding process is that full bidding feedback information is released to all competing bidders (and public). The specific objectives are: (i) to examine the degree of competition in the construction markets; and (ii) to examine the bidding performance of competing bidders. Based on a collection of bidding data for a 15-month period, the results show the market environment of the Singapore public sector construction contracting is highly competitive with long bidder lists. In selection of contractors, only 50% of the contracts were awarded to lowest bidders. The results also show that the competing contractors can be broadly classified into three groups based on their bidding performance in terms of number of bidding attempts, bidding success rate and bidding competitiveness. These results provide a useful insight into the bidding competition in the Singapore public sector construction contracting, especially to new market entrants and foreign contractors who want to bid for jobs in Singapore.

Protecting the Environment Through Public Procurement Law - The Case of Poland

NASA Astrophysics Data System (ADS)

Kozik, Renata; Karasińska-Jaśkowiec, Izabela

2016-10-01

The article presents the results of studies whose aim was to analyse public procurement procedures in the context of environmental protection, especially wastewater infrastructure, performed in the selected Polish municipalities. The selection criterions were the time of public procurement procedure - from 2009 until 2015 and that the contract was cofinanced by the European Union funds. The contracting authorities responsible for awarding specific contracts, were asked series of detailed questions about the environmental criteria and requirements contained in the tender documents for the construction contracts related to the wastewater infrastructure. The aim of the detailed study was to determine whether the actions taken by the contracting authorities in a tender procedure for the construction or operation of wastewater infrastructure include environmental issues. Authors examine also the applicable public procurement law regulation in Poland and Europe in the context of sustainable development and environmental protection. The study of public procurement law was to check whether the law regulation fully takes into account the environmental aspects of the planned investments. On this basis, conclusions have been made that laws are consistent and do not constitute an obstacle to awarding a contract positively affecting the environment.

A normal incidence, high resolution X-ray telescope for solar coronal observations

NASA Technical Reports Server (NTRS)

Golub, L.

1984-01-01

Efforts directed toward the completion of an X-ray telescope assembly design, the procurement of major components, and the coordination of optical fabrication and X-ray multilayer testing are reported.

40 CFR 35.925-20 - Procurement.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Procurement. 35.925-20 Section 35.925-20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.925-20...

40 CFR 35.925-20 - Procurement.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Procurement. 35.925-20 Section 35.925-20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.925-20...

Environmental Criteria in the Spanish Public Works Procurement Process.

PubMed

Fuentes-Bargues, José Luis; González-Cruz, Mª Carmen; González-Gaya, Cristina

2017-02-18

Green Public Procurement (GPP) is defined as a process of contracting products, services, and works with the least possible damage to the environment during their life cycle. In order to improve the knowledge about GPP, a study of the use of environmental tendering criteria in the Spanish public construction sector has been performed. The results of this study show that the use of environmental criteria in Spanish public sector construction procurement is low in comparison to a certain group of countries, known as "Green 7", in the European Union. Environmental criteria is the fourth criterion in importance, but its weight in the global of the process is much lower than other criteria such as price, memory of the construction process and the delivery time. National administrations use environmental criteria more frequently because they have more resources and staff training about environmental issues. Environmental criteria are more used in the tendering of civil projects and works whose budget exceeds ten million euro due to the environmental impact of these kind and/or size of projects.

77 FR 44065 - Federal Acquisition Regulation; Technical Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-26

... FAR. List of Subjects in 48 CFR Parts 1, 16, 22, and 52 Government procurement. Dated: July 16, 2012...--APPLICATION OF LABOR LAWS TO GOVERNMENT ACQUISITIONS 22.1801 [Amended] 0 4. Amend section 22.1801 by-- 0 a... Affirmative Procurement of Biobased Products Under Service and Construction Contracts. * * * * * Affirmative...

Procurement Forms | NREL

Science.gov Websites

Certificate of Current Cost or Pricing Data Construction Specific Forms Pre-Award Documents Construction and manuals) Cost and Pricing Data Definitions Design-Build / Price Proposal Form Information

ProCure21+ should speed scheme starts.

PubMed

Baillie, Jonathan

2010-11-01

This October saw the launch of the new ProCure21+ National Framework under which, the Department of Health (DH) team behind the new scheme claims, the NHS can potentially save a further pound 200 million of public money on top of the substantial sums saved under predecessor, ProCure21, via faster, more streamlined procurement, design, planning, and construction, of publicly-funded healthcare schemes. HEJ editor Jonathan Baillie discussed, with the DH's senior responsible officer (SRO) and P21+ team leader Peter Sellars, the background to the new Framework's introduction, the success of its forerunner, and the additional benefits that ProCure21 + (which is backed by organisations incuding HM Treasury, the National Audit Office, and the Office of Government Commerce) should bring to the entire healthcare building supply chain.

48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

Code of Federal Regulations, 2013 CFR

2013-10-01

... management and operating contracts or other contracts as determined by the Senior Procurement Executive, including those using the Construction, Construction Management, or Special Equipment Purchases/Subcontract... for construction and construction management contracts). However, the base amount should not normally...

48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

Code of Federal Regulations, 2012 CFR

2012-10-01

... management and operating contracts or other contracts as determined by the Senior Procurement Executive, including those using the Construction, Construction Management, or Special Equipment Purchases/Subcontract... for construction and construction management contracts). However, the base amount should not normally...

48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

Code of Federal Regulations, 2014 CFR

2014-10-01

... management and operating contracts or other contracts as determined by the Senior Procurement Executive, including those using the Construction, Construction Management, or Special Equipment Purchases/Subcontract... for construction and construction management contracts). However, the base amount should not normally...

48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

Code of Federal Regulations, 2011 CFR

2011-10-01

... management and operating contracts or other contracts as determined by the Senior Procurement Executive, including those using the Construction, Construction Management, or Special Equipment Purchases/Subcontract... for construction and construction management contracts). However, the base amount should not normally...

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

The Procurement and Assistance Data System (PADS) provides a mechanism for collecting, assembling, organizing, and presenting acquisition and assistance data for the Department of Energy (DOE). This handbook and associated documents establish a uniform system to report acquisition/assistance data to PADS for the collection, processing, and dissemination of official statistical data on the Department`s acquisition and assistance actions. The data provides, based on information available at the time of request, a basis for any recurring and special reports to the senior procurement executive, Congress, General Accounting Office (GAO), Federal executive agencies, Office of management and Budget (OMB), and the generalmore » public. It provides information for measuring and assessing the extent to which small business firms and small disadvantages business enterprises are sharing in DOE placement, and for other acquisition and assistance policy and management control purposes. The Handbook for the Preparation of the Individual Procurement Action Report (IPAR) has been designed in two sections; Procurement and Financial Assistance to support the individual forms.« less

Competition in the economic crisis: Analysis of procurement auctions.

PubMed

Gugler, Klaus; Weichselbaumer, Michael; Zulehner, Christine

2015-01-01

We study the effects of the recent economic crisis on firms׳ bidding behavior and markups in sealed bid auctions. Using data from Austrian construction procurements, we estimate bidders׳ construction costs within a private value auction model. We find that markups of all bids submitted decrease by 1.5 percentage points in the recent economic crisis, markups of winning bids decrease by 3.3 percentage points. We also find that without the government stimulus package this decrease would have been larger. These two pieces of evidence point to pro-cyclical markups.

78 FR 23543 - Procurement List Deletions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-19

... Logistics Agency Troop Support, Philadelphia, PA NSN: 1680-00-677-2060--Bottom Assembly, Crew Berth NPA: None assigned. Contracting Activity: Defense Logistics Agency Aviation, Richmond, VA Service Service... Area Rehabilitation Centers, Inc., Madison, WI Contracting Activity: GSA, Public Buildings Service...

A Conceptual Framework for Procurement Decision Making Model to Optimize Supplier Selection: The Case of Malaysian Construction Industry

NASA Astrophysics Data System (ADS)

Chuan, Ngam Min; Thiruchelvam, Sivadass; Nasharuddin Mustapha, Kamal; Che Muda, Zakaria; Mat Husin, Norhayati; Yong, Lee Choon; Ghazali, Azrul; Ezanee Rusli, Mohd; Itam, Zarina Binti; Beddu, Salmia; Liyana Mohd Kamal, Nur

2016-03-01

This paper intends to fathom the current state of procurement system in Malaysia specifically in the construction industry in the aspect of supplier selection. This paper propose a comprehensive study on the supplier selection metrics for infrastructure building, weight the importance of each metrics assigned and to find the relationship between the metrics among initiators, decision makers, buyers and users. With the metrics hierarchy of criteria importance, a supplier selection process can be defined, repeated and audited with lesser complications or difficulties. This will help the field of procurement to improve as this research is able to develop and redefine policies and procedures that have been set in supplier selection. Developing this systematic process will enable optimization of supplier selection and thus increasing the value for every stakeholders as the process of selection is greatly simplified. With a new redefined policy and procedure, it does not only increase the company’s effectiveness and profit, but also make it available for the company to reach greater heights in the advancement of procurement in Malaysia.

Space shuttle main engine controller assembly, phase C-D. [with lagging system design and analysis

NASA Technical Reports Server (NTRS)

1973-01-01

System design and system analysis and simulation are slightly behind schedule, while design verification testing has improved. Input/output circuit design has improved, but digital computer unit (DCU) and mechanical design continue to lag. Part procurement was impacted by delays in printed circuit board, assembly drawing releases. These are the result of problems in generating suitable printed circuit artwork for the very complex and high density multilayer boards.

49 CFR 661.11 - Rolling stock procurements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Devices; (21) Car Axle Counters; (22) Communication Based Train Control (CBTC). (u) Communication... components of a bus. This list is not all-inclusive. Car body shells, egines, transmissions, front axle... assemblies, air compressor and pneumatic systems, generator/alternator and electrical systems, steering...

49 CFR 661.11 - Rolling stock procurements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Devices; (21) Car Axle Counters; (22) Communication Based Train Control (CBTC). (u) Communication... components of a bus. This list is not all-inclusive. Car body shells, egines, transmissions, front axle... assemblies, air compressor and pneumatic systems, generator/alternator and electrical systems, steering...

Environmental Criteria in the Spanish Public Works Procurement Process

PubMed Central

Fuentes-Bargues, José Luis; González-Cruz, Mª Carmen; González-Gaya, Cristina

2017-01-01

Green Public Procurement (GPP) is defined as a process of contracting products, services, and works with the least possible damage to the environment during their life cycle. In order to improve the knowledge about GPP, a study of the use of environmental tendering criteria in the Spanish public construction sector has been performed. The results of this study show that the use of environmental criteria in Spanish public sector construction procurement is low in comparison to a certain group of countries, known as “Green 7”, in the European Union. Environmental criteria is the fourth criterion in importance, but its weight in the global of the process is much lower than other criteria such as price, memory of the construction process and the delivery time. National administrations use environmental criteria more frequently because they have more resources and staff training about environmental issues. Environmental criteria are more used in the tendering of civil projects and works whose budget exceeds ten million euro due to the environmental impact of these kind and/or size of projects. PMID:28218706

Red Plague Control Plan (RPCP)

NASA Technical Reports Server (NTRS)

Cooke, Robert W.

2010-01-01

SCOPE: Prescribes the minimum requirements for the control of cuprous / cupric oxide corrosion (a.k.a. Red Plague) of silver-coated copper wire, cable, and harness assemblies. PURPOSE: Targeted for applications where exposure to assembly processes, environmental conditions, and contamination may promote the development of cuprous / cupric oxide corrosion (a.k.a. Red Plague) in silver-coated copper wire, cable, and harness assemblies. Does not exclude any alternate or contractor-proprietary documents or processes that meet or exceed the baseline of requirements established by this document. Use of alternate or contractor-proprietary documents or processes shall require review and prior approval of the procuring NASA activity.

78 FR 76700 - Procurement Thresholds for Implementation of the Trade Agreements Act of 1979

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-18

...-Oman Free Trade Agreement (United States-Oman FTA), Chapter 9 of the United States- Panama Trade... FTA, Chapter 10 of NAFTA, Chapter 9 of the United States-Oman FTA, Chapter 9 of the United States...) Procurement of construction services--$12,721,740. IX. United States-Oman FTA, Chapter 9 A. Central Level...

48 CFR 736.602-5 - Short selection process for procurements not to exceed the simplified acquisition threshold.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Short selection process for procurements not to exceed the simplified acquisition threshold. 736.602-5 Section 736.602-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SPECIAL CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACT...

48 CFR 736.602-5 - Short selection process for procurements not to exceed the simplified acquisition threshold.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Short selection process for procurements not to exceed the simplified acquisition threshold. 736.602-5 Section 736.602-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SPECIAL CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACT...

48 CFR 736.602-5 - Short selection process for procurements not to exceed the simplified acquisition threshold.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Short selection process for procurements not to exceed the simplified acquisition threshold. 736.602-5 Section 736.602-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SPECIAL CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACT...

Composite Bus Structure for the SMEX/WIRE Satellite

NASA Technical Reports Server (NTRS)

Rosanova, Giulio G.

1998-01-01

In an effort to reduce the weight and optimize the structural design of the Small Explorer (SMEX) Wide-Field Infrared Explorer (WIRE) spacecraft, it has become desirable to change the material and construction from mechanically fastened aluminum structure to a fully bonded fiber-reinforced composite (FRC) structure. GSFC has developed the WIRE spacecraft structural bus design concept, including the instrument and launch vehicle requirements. The WIRE Satellite is the fifth of a series of SMEX satellites to be launched once per year. GSFC has chosen Composite Optics Inc. (COI) as the prime contractor for the development and procurement of the WIRE composite structure. The detailed design of the fully bonded FRC structure is based on COI's Short Notice Accelerated Production SATellite ("SNAPSAT") approach. SNAPSAT is a state of the art design and manufacturing technology for advanced composite materials which utilizes flat-stock detail parts bonded together to produce a final structural assembly. The structural design approach adopted for the WIRE structure provides a very viable alternative to both traditional aluminum construction as well as high tech. molded type composite structures. This approach to composite structure design is much less costly than molded or honeycomb sandwich type composite construction, but may cost slightly more than conventional aluminum construction on the subsystem level. However on the overall program level the weight saving achieved is very cost effective, since the primary objective is to allocate more mass for science payloads.

Automated Glycan Assembly of Oligosaccharides Related to Arabinogalactan Proteins.

PubMed

Bartetzko, Max P; Schuhmacher, Frank; Hahm, Heung Sik; Seeberger, Peter H; Pfrengle, Fabian

2015-09-04

Arabinogalactan proteins are heavily glycosylated proteoglycans in plants. Their glycan portion consists of type-II arabinogalactan polysaccharides whose heterogeneity hampers the assignment of the arabinogalactan protein function. Synthetic chemistry is key to the procurement of molecular probes for plant biologists. Described is the automated glycan assembly of 14 oligosaccharides from four monosaccharide building blocks. These linear and branched glycans represent key structural features of natural type-II arabinogalactans and will serve as tools for arabinogalactan biology.

Evaluation of the US Army Fitting Program for the Integrated Helmet Unit of the Integrated Helmet and Display Sighting System

DTIC Science & Technology

1987-07-01

ederick, MD 21701-5012- M WPUM TPOSONINGOFFICE SYMBOL 9 PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANTICIN(i soppkablo) &L. ADUSS (City umat . and W...distortion, improperly positioned helmet electronics, or damaged HDU receiver assembly, 9 and HDU optical axis misalignment due to a bent combiner or...internal damage to the HDU lens assembly. All components were contractoLr supplied. The scheduling of a fitting session required coordination between

Department of the Army Justification of Estimates for Fiscal Year 1986. Procurement Appropriations-Construction Program Submitted to Congress February 1985. Part 1. DD Forms 1391.

DTIC Science & Technology

1985-02-01

CATALOG NUMBER 4. TITLE (and Subtitle)(in 6 parts) Department of the Army S. TYPE OF REPORT & PERIOD COVERED Justification of Estimates for Fiscal Year... 6 - PERFORMING ORG. REPORT NUMBER Veh., Ammunition, Other Procurement & Constr Proer ms 7. AUTHOR(a) 8. CONTRACT OR GRANT NUMBER(&) 5 Department of...I ry 19e 6 MILITAP. Y CONSTRUCTION PRO3[CT DATA I-.-1 005 I .. . . .. . .LL .... _ I . a,.-ta13st an and toction I A.. Project Title St rttford Arcy

Codes Don't Always Get Enforced, But Contracts Do: Changing the Procurement Paradigm to Drive Building Energy Performance

DOE Office of Scientific and Technical Information (OSTI.GOV)

Torcellini, Paul A; Scheib, Jennifer G; Pless, Shanti

New construction could account for more than 25% of the U.S. energy consumption by 2030. Millions of square feet are built every year that will not perform as expected - despite advancing codes, rating systems, super-efficient technologies, and advanced utility programs. With retrofits of these under-performers decades away, savings potential will be lost for years to come. Only the building owner is in the driver's seat to demand - and verify - higher-performing buildings. Yet our current policy and market interventions really target the design team, not the owner. Accelerate Performance, a U.S. Department of Energy funded initiative, is changingmore » the building procurement approach to drive deeper, verified savings in three pilot states: Illinois, Minnesota, and Connecticut. Performance-based procurement ties energy performance to design and contractor team compensation while freeing them to meet energy targets with strategies most familiar to them. The process teases out the creativity of the design and contracting teams to deliver energy performance - without driving up the construction cost. The paper will share early results and lessons learned from new procurement and contract approaches in government, public, and private sector building projects. The paper provides practical guidance for building owners, facilities managers, design, and contractor teams who wish to incorporate effective performance-based procurement for deeper energy savings in their buildings.« less

National Ignition Facility project acquisition plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Callaghan, R.W.

The purpose of this National Ignition Facility Acquisition Plan is to describe the overall procurement strategy planned for the National Ignition Facility (NIF) Project. The scope of the plan describes the procurement activities and acquisition strategy for the following phases of the NIF Project, each of which receives either plant and capital equipment (PACE) or other project cost (OPC) funds: Title 1 and 2 design and Title 3 engineering (PACE); Optics manufacturing facilitization and pilot production (OPC); Convention facility construction (PACE); Procurement, installation, and acceptance testing of equipment (PACE); and Start-up (OPC). Activities that are part of the base Inertialmore » Confinement Fusion (ICF) Program are not included in this plan. The University of California (UC), operating Lawrence Livermore National Laboratory (LLNL) and Los Alamos National Laboratory, and Lockheed-Martin, which operates Sandia National Laboratory (SNL) and the University of Rochester Laboratory for Laser Energetics (UR-LLE), will conduct the acquisition of needed products and services in support of their assigned responsibilities within the NIF Project structure in accordance with their prime contracts with the Department of Energy (DOE). LLNL, designated as the lead Laboratory, will have responsibility for all procurements required for construction, installation, activation, and startup of the NIF.« less

SMEX-Lite Modular Solar Array Architecture

NASA Technical Reports Server (NTRS)

Lyons, John

2002-01-01

For the most part, Goddard solar arrays have been custom designs that are unique to each mission. The solar panel design has been frozen prior to issuing an RFP for their procurement. There has typically been 6-9 months between RFP release and contract award, followed by an additional 24 months for performance of the contract. For Small Explorer (SMEX) missions, with three years between mission definition and launch, this has been a significant problem. The SMEX solar panels have been sufficiently small that the contract performance period has been reduced to 12-15 months. The bulk of this time is used up in the final design definition and fabrication of flight solar cell assemblies. Even so, it has been virtually impossible to have the spacecraft design at a level of maturity sufficient to freeze the solar panel geometry and release the RFP in time to avoid schedule problems with integrating the solar panels to the spacecraft. With that in mind, the SMEX-Lite project team developed a modular architecture for the assembly of solar arrays to greatly reduce the cost and schedule associated with the development of a mission- specific solar array. In the modular architecture, solar cells are fabricated onto small substrate panels. This modular panel (approximately 8.5" x 17" in this case) becomes the building block for constructing solar arrays for multiple missions with varying power requirements and geometrical arrangements. The mechanical framework that holds these modules together as a solar array is the only mission-unique design, changing in size and shape as required for each mission. There are several advantages to this approach. First, the typical solar array development cycle requires a mission unique design, procurement, and qualification including a custom qualification panel. With the modular architecture, a single qualification of the SMEX-Lite modules and the associated mechanical framework in a typical configuration provided a qualification by similarity to multiple missions. It then becomes possible to procure solar array modules in advance of mission definition and respond quickly and inexpensively to a selected mission's unique requirements. The solar array modular architecture allows the procurement of solar array modules before the array geometry has been frozen. This reduces the effect of procurement lead-time on the mission integration and test flow by as much as 50%. Second, by spreading the non-recurring costs over multiple missions, the cost per unit area is also reduced. In the case of the SMEX-Lite procurement, this reduction was by about one third of the cost per unit area compared to previous SMEX mission-unique procurements. Third, the modular architecture greatly facilitates the infusion of new solar cell technologies into flight programs as these technologies become available. New solar cell technologies need only be fabricated onto a standard-sized module to be incorporated into the next available mission. The modular solar array can be flown in a mixed configuration with some new and some standard cell technologies. Since each module has its own wiring terminals, the array can be arranged as desired electrically with little impact to cost and schedule. The solar array modular architecture does impose some additional constraints on systems and subsystem engineers. First, they must work with discrete solar array modules rather than size the array to fit exactly within an available envelope. The array area is constrained to an integer multiple of the module area. Second, the modular design is optimized for space radiation and thermal environments not greatly different from a typical SMEX LEO environment. For example, a mission with a highly elliptical orbit (e.g., Polar, SMEX/FAST) would require thicker coverglasses to protect the solar cells from the more intense radiation environment.

SMEX-Lite Modular Solar Array Architecture

NASA Technical Reports Server (NTRS)

Lyons, John W.; Day, John (Technical Monitor)

2002-01-01

The NASA Small Explorer (SMEX) missions have typically had three years between mission definition and launch. This short schedule has posed significant challenges with respect to solar array design and procurement. Typically, the solar panel geometry is frozen prior to going out with a procurement. However, with the SMEX schedule, it has been virtually impossible to freeze the geometry in time to avoid scheduling problems with integrating the solar panels to the spacecraft. A modular solar array architecture was developed to alleviate this problem. This approach involves procuring sufficient modules for multiple missions and assembling the modules onto a solar array framework that is unique to each mission. The modular approach removes the solar array from the critical path of the SMEX integration and testing schedule. It also reduces the cost per unit area of the solar arrays and facilitates the inclusion of experiments involving new solar cell or panel technologies in the SMEX missions.

13 CFR 124.507 - What procedures apply to competitive 8(a) procurements?

Code of Federal Regulations, 2011 CFR

2011-01-01

... developmental and transitional stages of program participation. (2) Construction competitions. Based on its knowledge of the 8(a) BD portfolio, SBA will determine whether a competitive 8(a) construction requirement... construction. (3) If SBA determines that the apparent successful offeror is ineligible, SBA will notify the...

22 CFR 228.17 - Special procurement rules for construction and engineering services.

Code of Federal Regulations, 2012 CFR

2012-04-01

... engineering services. 228.17 Section 228.17 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT RULES FOR... construction and engineering services. Advanced developing countries, as defined in § 228.01, which USAID has... engineering services are not eligible to furnish USAID-financed construction and engineering services unless...

22 CFR 228.17 - Special procurement rules for construction and engineering services.

Code of Federal Regulations, 2013 CFR

2013-04-01

... engineering services. 228.17 Section 228.17 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT RULES FOR... construction and engineering services. Advanced developing countries, as defined in § 228.01, which USAID has... engineering services are not eligible to furnish USAID-financed construction and engineering services unless...

22 CFR 228.17 - Special procurement rules for construction and engineering services.

Code of Federal Regulations, 2014 CFR

2014-04-01

... engineering services. 228.17 Section 228.17 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT RULES FOR... construction and engineering services. Advanced developing countries, as defined in § 228.01, which USAID has... engineering services are not eligible to furnish USAID-financed construction and engineering services unless...

The ITER project construction status

NASA Astrophysics Data System (ADS)

Motojima, O.

2015-10-01

The pace of the ITER project in St Paul-lez-Durance, France is accelerating rapidly into its peak construction phase. With the completion of the B2 slab in August 2014, which will support about 400 000 metric tons of the tokamak complex structures and components, the construction is advancing on a daily basis. Magnet, vacuum vessel, cryostat, thermal shield, first wall and divertor structures are under construction or in prototype phase in the ITER member states of China, Europe, India, Japan, Korea, Russia, and the United States. Each of these member states has its own domestic agency (DA) to manage their procurements of components for ITER. Plant systems engineering is being transformed to fully integrate the tokamak and its auxiliary systems in preparation for the assembly and operations phase. CODAC, diagnostics, and the three main heating and current drive systems are also progressing, including the construction of the neutral beam test facility building in Padua, Italy. The conceptual design of the Chinese test blanket module system for ITER has been completed and those of the EU are well under way. Significant progress has been made addressing several outstanding physics issues including disruption load characterization, prediction, avoidance, and mitigation, first wall and divertor shaping, edge pedestal and SOL plasma stability, fuelling and plasma behaviour during confinement transients and W impurity transport. Further development of the ITER Research Plan has included a definition of the required plant configuration for 1st plasma and subsequent phases of ITER operation as well as the major plasma commissioning activities and the needs of the accompanying R&D program to ITER construction by the ITER parties.

Development and fabrication of a solar cell junction processing system

NASA Technical Reports Server (NTRS)

Kiesling, R.

1981-01-01

The major component fabrication program was completed. Assembly and system testing of the pulsed electron beam annealing machine are described. The design program for the transport reached completion, and the detailed drawings were released for fabrication and procurement of the long lead time components.

76 FR 76808 - Procurement Thresholds for Implementation of the Trade Agreements Act of 1979

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-08

...), Chapter 9 of the United States-Peru Trade Promotion Agreement (U.S.-Peru TPA), and Chapter 13 of the..., Chapter 9 of the U.S.-Peru TPA, and Chapter 13 of the U.S.-Singapore FTA, do hereby determine, effective... Entities-- $622,000; (2) Procurement of construction services--$12,399,671. IX. U.S.-Peru TPA, Chapter 9 A...

32 CFR 634.4 - Responsibilities.

Code of Federal Regulations, 2011 CFR

2011-07-01

... installations by participating in traffic control studies designed to obtain information on traffic problems and... phase of engineering concerned with the planning, design, construction, and maintenance of streets, highways, and abutting lands. (2) Select, determine appropriate design, procure, construct, install, and...

32 CFR 634.4 - Responsibilities.

Code of Federal Regulations, 2014 CFR

2014-07-01

... installations by participating in traffic control studies designed to obtain information on traffic problems and... phase of engineering concerned with the planning, design, construction, and maintenance of streets, highways, and abutting lands. (2) Select, determine appropriate design, procure, construct, install, and...

32 CFR 634.4 - Responsibilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... installations by participating in traffic control studies designed to obtain information on traffic problems and... phase of engineering concerned with the planning, design, construction, and maintenance of streets, highways, and abutting lands. (2) Select, determine appropriate design, procure, construct, install, and...

32 CFR 634.4 - Responsibilities.

Code of Federal Regulations, 2013 CFR

2013-07-01

... installations by participating in traffic control studies designed to obtain information on traffic problems and... phase of engineering concerned with the planning, design, construction, and maintenance of streets, highways, and abutting lands. (2) Select, determine appropriate design, procure, construct, install, and...

32 CFR 634.4 - Responsibilities.

Code of Federal Regulations, 2012 CFR

2012-07-01

... installations by participating in traffic control studies designed to obtain information on traffic problems and... phase of engineering concerned with the planning, design, construction, and maintenance of streets, highways, and abutting lands. (2) Select, determine appropriate design, procure, construct, install, and...

A Cost-Minimization Analysis of Tissue-Engineered Constructs for Corneal Endothelial Transplantation

PubMed Central

Tan, Tien-En; Peh, Gary S. L.; George, Benjamin L.; Cajucom-Uy, Howard Y.; Dong, Di; Finkelstein, Eric A.; Mehta, Jodhbir S.

2014-01-01

Corneal endothelial transplantation or endothelial keratoplasty has become the preferred choice of transplantation for patients with corneal blindness due to endothelial dysfunction. Currently, there is a worldwide shortage of transplantable tissue, and demand is expected to increase further with aging populations. Tissue-engineered alternatives are being developed, and are likely to be available soon. However, the cost of these constructs may impair their widespread use. A cost-minimization analysis comparing tissue-engineered constructs to donor tissue procured from eye banks for endothelial keratoplasty was performed. Both initial investment costs and recurring costs were considered in the analysis to arrive at a final tissue cost per transplant. The clinical outcomes of endothelial keratoplasty with tissue-engineered constructs and with donor tissue procured from eye banks were assumed to be equivalent. One-way and probabilistic sensitivity analyses were performed to simulate various possible scenarios, and to determine the robustness of the results. A tissue engineering strategy was cheaper in both investment cost and recurring cost. Tissue-engineered constructs for endothelial keratoplasty could be produced at a cost of US$880 per transplant. In contrast, utilizing donor tissue procured from eye banks for endothelial keratoplasty required US$3,710 per transplant. Sensitivity analyses performed further support the results of this cost-minimization analysis across a wide range of possible scenarios. The use of tissue-engineered constructs for endothelial keratoplasty could potentially increase the supply of transplantable tissue and bring the costs of corneal endothelial transplantation down, making this intervention accessible to a larger group of patients. Tissue-engineering strategies for corneal epithelial constructs or other tissue types, such as pancreatic islet cells, should also be subject to similar pharmacoeconomic analyses. PMID:24949869

A cost-minimization analysis of tissue-engineered constructs for corneal endothelial transplantation.

PubMed

Tan, Tien-En; Peh, Gary S L; George, Benjamin L; Cajucom-Uy, Howard Y; Dong, Di; Finkelstein, Eric A; Mehta, Jodhbir S

2014-01-01

Corneal endothelial transplantation or endothelial keratoplasty has become the preferred choice of transplantation for patients with corneal blindness due to endothelial dysfunction. Currently, there is a worldwide shortage of transplantable tissue, and demand is expected to increase further with aging populations. Tissue-engineered alternatives are being developed, and are likely to be available soon. However, the cost of these constructs may impair their widespread use. A cost-minimization analysis comparing tissue-engineered constructs to donor tissue procured from eye banks for endothelial keratoplasty was performed. Both initial investment costs and recurring costs were considered in the analysis to arrive at a final tissue cost per transplant. The clinical outcomes of endothelial keratoplasty with tissue-engineered constructs and with donor tissue procured from eye banks were assumed to be equivalent. One-way and probabilistic sensitivity analyses were performed to simulate various possible scenarios, and to determine the robustness of the results. A tissue engineering strategy was cheaper in both investment cost and recurring cost. Tissue-engineered constructs for endothelial keratoplasty could be produced at a cost of US$880 per transplant. In contrast, utilizing donor tissue procured from eye banks for endothelial keratoplasty required US$3,710 per transplant. Sensitivity analyses performed further support the results of this cost-minimization analysis across a wide range of possible scenarios. The use of tissue-engineered constructs for endothelial keratoplasty could potentially increase the supply of transplantable tissue and bring the costs of corneal endothelial transplantation down, making this intervention accessible to a larger group of patients. Tissue-engineering strategies for corneal epithelial constructs or other tissue types, such as pancreatic islet cells, should also be subject to similar pharmacoeconomic analyses.

75 FR 5033 - Departmental Management; Public meeting on BioPreferredSM

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-01

...; Public meeting on BioPreferred\\SM\\ Complex Products and Assemblies Designation and Industry Training on... include: 1. Using BioPreferred tools to position your business to sell or increase sales to the Federal...: Ron Buckhalt, BioPreferred Manager, U.S. Department of Agriculture, Office of Procurement and Property...

48 CFR 9904.417 - Cost of money as an element of the cost of capital assets under construction.

Code of Federal Regulations, 2010 CFR

2010-10-01

... element of the cost of capital assets under construction. 9904.417 Section 9904.417 Federal Acquisition Regulations System COST ACCOUNTING STANDARDS BOARD, OFFICE OF FEDERAL PROCUREMENT POLICY, OFFICE OF MANAGEMENT... of money as an element of the cost of capital assets under construction. ...

75 FR 13765 - Submission for OMB Review; Use of Project Labor Agreements for Federal Construction Projects

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-23

... a project labor agreement (PLA), as they may decide appropriate, on large-scale construction... efficiency in Federal procurement. A PLA is a pre-hire collective bargaining agreement with one or more labor...

Vietnam Report, Number 2393, Tap Chi Cong San, Number 6, June 1982.

DTIC Science & Technology

1982-09-14

PROCUREMENT OF PUBLICATIONS JPRS publications may be ordered from the National Technical Information Service, Springfield, Virginia 22161. In order- ing, it...to matters other than procurement may be addressed to Joint Publications Research Service, 1000 North Glebe Road, Arlington, Virginia 22201. JPRS...concentrate all efforts on preventing the other side from making a wrong move, thereby formulating a constructive solution to the replacement of the arms

Price vs. Performance: The Value of Next Generation Fighter Aircraft

DTIC Science & Technology

2007-03-01

forms. Both the semi-log and log-log forms were plagued with heteroskedasticity (according to the Breusch - Pagan /Cook-Weisberg test ). The RDT&E models...from 1949-present were used to construct two models – one based on procurement costs and one based on research, design, test , and evaluation (RDT&E...fighter aircraft hedonic models include several different categories of variables. Aircraft procurement costs and research, design, test , and

Management systems research study

NASA Technical Reports Server (NTRS)

Bruno, A. V.

1975-01-01

The development of a Monte Carlo simulation of procurement activities at the NASA Ames Research Center is described. Data cover: simulation of the procurement cycle, construction of a performance evaluation model, examination of employee development, procedures and review of evaluation criteria for divisional and individual performance evaluation. Determination of the influences and apparent impact of contract type and structure and development of a management control system for planning and controlling manpower requirements.

Current Chinese Leadership Update

DTIC Science & Technology

1985-08-01

Horst Soelle fontacts East German Minister for the Construction of Heavy Machinery and Equipment Rolf Kersten 6 Contacts East German Deputy Minister for...Minister for Defense for Procurement Adam Butler Ana_1sis Commander of the CPLA Air Force Zhang Tingfa visited 9 France (29 May-9 June 1985) and the...Chief Marshal Sir Keith Williamson, MinisteL of Defense for Procurement Adam Butler, and other unidentified British military officials. Zhang visited

Loglines. May - June 2010

DTIC Science & Technology

2010-06-01

able to get construction materials, food and spare parts into a land-locked country . More than 80 percent of the containers shipped through this...procurement management at DLA Oklahoma City, has developed a strategic roadmap for DLR decision-makers. “Trying to get our arms around all the contracts...currently in place and develop - ing a comprehensive approach to future support has been challenging,” Yort said. Transferring procurement of DLRs to

INNOVATIVE FOSSIL FUEL FIRED VITRIFICATION TECHNOLOGY FOR SOIL REMEDIATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

J. Hnat; L.M. Bartone; M. Pineda

2001-07-13

This Summary Report summarizes the progress of Phases 3, 3A and 4 of a waste technology Demonstration Project sponsored under a DOE Environmental Management Research and Development Program and administered by the U.S. Department of Energy National Energy Technology Laboratory-Morgantown (DOE-NETL) for an ''Innovative Fossil Fuel Fired Vitrification Technology for Soil Remediation''. The Summary Reports for Phases 1 and 2 of the Program were previously submitted to DOE. The total scope of Phase 3 was to have included the design, construction and demonstration of Vortec's integrated waste pretreatment and vitrification process for the treatment of low level waste (LLW), TSCA/LLWmore » and mixed low-level waste (MLLW). Due to funding limitations and delays in the project resulting from a law suit filed by an environmental activist and the extended time for DOE to complete an Environmental Assessment for the project, the scope of the project was reduced to completing the design, construction and testing of the front end of the process which consists of the Material Handling and Waste Conditioning (MH/C) Subsystem of the vitrification plant. Activities completed under Phases 3A and 4 addressed completion of the engineering, design and documentation of the Material Handling and Conditioning System such that final procurement of the remaining process assemblies can be completed and construction of a Limited Demonstration Project be initiated in the event DOE elects to proceed with the construction and demonstration testing of the MH/C Subsystem.« less

7 CFR 1726.301 - Borrower contractual obligations.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES RUS Standard Forms § 1726.301... which the borrower agrees to use RUS standard forms of contracts for construction, procurement... agreements as implemented by this part, borrowers must use those forms of contract (hereinafter sometimes...

Fabrication and assembly of the ERDA/NASA 100 kilowatt experimental wind turbine

NASA Technical Reports Server (NTRS)

Puthoff, R. L.

1976-01-01

As part of the Energy Research and Development Administration (ERDA) wind-energy program, NASA Lewis Research Center has designed and built an experimental 100-kW wind turbine. The two-bladed turbines drives a synchronous alternator that generates its maximum output of 100 kW of electrical power in a 29-km/hr (18-mph) wind. The design and assembly of the wind turbine were performed at Lewis from components that were procured from industry. The machine was installed atop the tower on September 3, 1975.

Construction Management for Educational Facilities: Professional Services' Procurement and Competitive Bid Statutes.

ERIC Educational Resources Information Center

Goldblatt, Steven M.; Wood, R. Craig

Construction management is utilized when a school district engages a firm to coordinate a total project. The construction management seeks to save an owner time and cost primarily through better contractor coordination and project management. These services may include the planning and design phases of the project as well as the actual…

7 CFR 1755.510 - Construction and assembly unit drawings.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 11 2014-01-01 2014-01-01 false Construction and assembly unit drawings. 1755.510..., AND STANDARD CONTRACT FORMS § 1755.510 Construction and assembly unit drawings. (a) The construction and assembly unit drawings in this section shall be used by borrowers to assist the installer in...

7 CFR 1755.510 - Construction and assembly unit drawings.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 11 2013-01-01 2013-01-01 false Construction and assembly unit drawings. 1755.510..., AND STANDARD CONTRACT FORMS § 1755.510 Construction and assembly unit drawings. (a) The construction and assembly unit drawings in this section shall be used by borrowers to assist the installer in...

7 CFR 1755.510 - Construction and assembly unit drawings.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 11 2012-01-01 2012-01-01 false Construction and assembly unit drawings. 1755.510..., AND STANDARD CONTRACT FORMS § 1755.510 Construction and assembly unit drawings. (a) The construction and assembly unit drawings in this section shall be used by borrowers to assist the installer in...

7 CFR 1726.250 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Modifications to RUS Standard Contract Forms § 1726.250 General. RUS provides standard forms of contract for the procurement of materials, equipment, and construction and for contract amendments and various related forms for use by RUS borrowers. See § 1726.304 for...

Perimeter intrusion detection and assessment system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eaton, M.J.; Jacobs, J.; McGovern, D.E.

1977-11-01

To obtain an effective perimeter intrusion detection system requires careful sensor selection, procurement, and installation. The selection process involves a thorough understanding of the unique site features and how these features affect the performance of each type of sensor. It is necessary to develop procurement specifications to establish acceptable sensor performance limits. Careful explanation and inspection of critical installation dimensions is required during on-site construction. The implementation of these activities at a particular site is discussed.

One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease in Saccharomyces cerevisiae

PubMed Central

Kuijpers, Niels GA; Chroumpi, Soultana; Vos, Tim; Solis-Escalante, Daniel; Bosman, Lizanne; Pronk, Jack T; Daran, Jean-Marc; Daran-Lapujade, Pascale

2013-01-01

In vivo assembly of overlapping fragments by homologous recombination in Saccharomyces cerevisiae is a powerful method to engineer large DNA constructs. Whereas most in vivo assembly methods reported to date result in circular vectors, stable integrated constructs are often preferred for metabolic engineering as they are required for large-scale industrial application. The present study explores the potential of combining in vivo assembly of large, multigene expression constructs with their targeted chromosomal integration in S. cerevisiae. Combined assembly and targeted integration of a ten-fragment 22-kb construct to a single chromosomal locus was successfully achieved in a single transformation process, but with low efficiency (5% of the analyzed transformants contained the correctly assembled construct). The meganuclease I-SceI was therefore used to introduce a double-strand break at the targeted chromosomal locus, thus to facilitate integration of the assembled construct. I-SceI-assisted integration dramatically increased the efficiency of assembly and integration of the same construct to 95%. This study paves the way for the fast, efficient, and stable integration of large DNA constructs in S. cerevisiae chromosomes. PMID:24028550

Robotic space construction

NASA Technical Reports Server (NTRS)

Mixon, Randolph W.; Hankins, Walter W., III; Wise, Marion A.

1988-01-01

Research at Langley AFB concerning automated space assembly is reviewed, including a Space Shuttle experiment to test astronaut ability to assemble a repetitive truss structure, testing the use of teleoperated manipulators to construct the Assembly Concept for Construction of Erectable Space Structures I truss, and assessment of the basic characteristics of manipulator assembly operations. Other research topics include the simultaneous coordinated control of dual-arm manipulators and the automated assembly of candidate Space Station trusses. Consideration is given to the construction of an Automated Space Assembly Laboratory to study and develop the algorithms, procedures, special purpose hardware, and processes needed for automated truss assembly.

An Analysis of Cost Premiums and Losses Associated with USAF Military Construction (MILCON)

DTIC Science & Technology

2013-03-01

Corps of Engineers (USACE) or the Naval Facilities Engineering Command (NAVFAC) for design and construction of the annual military construction...AFCEC in lieu of AFCEE or AFCESA. The selection, and policies, of design and construction agents may cause cost premiums for Air Force MILCON...private industry best practices such as relational contracting, schedule performance, and design -build procurement methods. All of these research

A case study : Michigan Intelligent Transportation System Center : use of a design/build/warranty contract

DOT National Transportation Integrated Search

2000-03-01

Many ITS projects are stand-alone in nature, and do not have to be procured under rules for construction. The installation of field devices and communications infrastructure often meets the definition of construction. However, if a project involves t...

U.S.-Coalition Forces and Host Nations: DOTmLPF-P for Contingency Procurements. Part 2. Conclusion

DTIC Science & Technology

2014-08-01

doesn’t have to be stood up. Examples include textile/cloth- ing/ footwear , construction, and furniture, industries that usu- ally exist in some...meet its needs but be within the HNVs’ capability to manufacture. Finally, materials used by HNVs to manufacture products under U.S. or HNG...procurement efforts should come from reliable sources with processes and physical features in place to prevent substandard or fake material from entering the

3 CFR 13502 - Executive Order 13502 of February 6, 2009. Use of Project Labor Agreements for Federal...

Code of Federal Regulations, 2010 CFR

2010-01-01

... developing by providing structure and stability to large-scale construction projects, thereby promoting the... procurement, producing labor-management stability, and ensuring compliance with laws and regulations governing... construction projects receiving Federal financial assistance, would help to promote the economical, efficient...

40 CFR 31.36 - Procurement.

Code of Federal Regulations, 2011 CFR

2011-07-01

... waste-water treatment works project and wishes to retain that firm or individual during construction of...) Construction grants awarded under Title II of the Clean Water Act are subject to the following “Buy American” requirements in paragraphs (c)(5) (i)-(iii) of this section. Section 215 of the Clean Water Act requires that...

76 FR 28333 - Electric Engineering, Architectural Services, Design Policies and Construction Standards

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-17

... CFR Parts 1724 and 1726 RIN 0572-AC20 Electric Engineering, Architectural Services, Design Policies... standard forms of contracts promulgated by RUS for construction, procurement, engineering services and... XVII of title 7 of the Code of Federal Regulations as follows: PART 1724--ELECTRIC ENGINEERING...

7 CFR 2902.17 - Plastic insulating foam for residential and commercial construction.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Plastic insulating foam for residential and... BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT Designated Items § 2902.17 Plastic insulating foam for residential and commercial construction. (a) Definition. Spray-in-place plastic foam products designed to...

7 CFR 3201.17 - Plastic insulating foam for residential and commercial construction.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 15 2014-01-01 2014-01-01 false Plastic insulating foam for residential and... DESIGNATING BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT Designated Items § 3201.17 Plastic insulating foam for residential and commercial construction. (a) Definition. Spray-in-place plastic foam products designed to...

7 CFR 3201.17 - Plastic insulating foam for residential and commercial construction.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 15 2012-01-01 2012-01-01 false Plastic insulating foam for residential and... DESIGNATING BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT Designated Items § 3201.17 Plastic insulating foam for residential and commercial construction. (a) Definition. Spray-in-place plastic foam products designed to...

7 CFR 3201.17 - Plastic insulating foam for residential and commercial construction.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 15 2013-01-01 2013-01-01 false Plastic insulating foam for residential and... DESIGNATING BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT Designated Items § 3201.17 Plastic insulating foam for residential and commercial construction. (a) Definition. Spray-in-place plastic foam products designed to...

Analysis of Criteria Influencing Contractor Selection Using TOPSIS Method

NASA Astrophysics Data System (ADS)

Alptekin, Orkun; Alptekin, Nesrin

2017-10-01

Selection of the most suitable contractor is an important process in public construction projects. This process is a major decision which may influence the progress and success of a construction project. Improper selection of contractors may lead to problems such as bad quality of work and delay in project duration. Especially in the construction projects of public buildings, the proper choice of contractor is beneficial to the public institution. Public procurement processes have different characteristics in respect to dissimilarities in political, social and economic features of every country. In Turkey, Turkish Public Procurement Law PPL 4734 is the main regulatory law for the procurement of the public buildings. According to the PPL 4734, public construction administrators have to contract with the lowest bidder who has the minimum requirements according to the criteria in prequalification process. Public administrators are not sufficient for selection of the proper contractor because of the restrictive provisions of the PPL 4734. The lowest bid method does not enable public construction administrators to select the most qualified contractor and they have realised the fact that the selection of a contractor based on lowest bid alone is inadequate and may lead to the failure of the project in terms of time delay Eand poor quality standards. In order to evaluate the overall efficiency of a project, it is necessary to identify selection criteria. This study aims to focus on identify importance of other criteria besides lowest bid criterion in contractor selection process of PPL 4734. In this study, a survey was conducted to staff of Department of Construction Works of Eskisehir Osmangazi University. According to TOPSIS (Technique for Order Preference by Similarity to the Ideal Solution) for analysis results, termination of construction work in previous tenders is the most important criterion of 12 determined criteria. The lowest bid criterion is ranked in rank 5.

Outlook on EDRS-C

NASA Astrophysics Data System (ADS)

Migliore, R.; Duncan, J.; Pulcino, V.; Bourne, D.; Voegt, S.; Perez, G.

2017-09-01

EDRS-C will operate on a geostationary orbit as the second node of the European Data Relay System (EDRS). The EDRS-C satellite, designed by OHB System as prime contractor, has been procured in the frame of a Public Private Partnership (PPP) between Airbus Defence and Space and ESA. EDRS-C is currently under assembly at OHB facilities in Bremen.

OAS - Organization of American States: Democracy for peace, security, and

Science.gov Websites

Elections Environment Equity G General Assembly Governance H Human Development Human Rights I Indigenous Quarterly Reports of the General Secretariat Procurement Human Resources Evaluation Internal Audit Real programmatic results. The full texts may be found here. Here you will find data on the Human Resources of the

Design and development of pressure and repressurization purge system for reusable space shuttle multilayer insulation system

NASA Technical Reports Server (NTRS)

1973-01-01

The manufacturing tasks for the program included the fabrication and assembly of an epoxy fiberglass purge bag to encapsulate an insulated cryogenic propellant tank. Purge, repressurization and venting hardware were procured and installed on the purge bag assembly in preparation for performance testing. The fabrication and installation of the superfloc multilayer insulation (MLI) on the cryogenic tank was accomplished as part of a continuing program. An abstraction of the results of the MLI fabrication task is included to describe the complete fabrication requirements for a reusable cryogenic propellant space storage system.

Interleaved arrays antenna technology development

NASA Technical Reports Server (NTRS)

1986-01-01

Phase one and two of a program to further develop and investigate advanced graphite epoxy waveguides, radiators, and components with application to space antennas are discussed. The objective of the two phases were to demonstrate mechanical integrity of a small panel of radiators and parts procured under a previous contract and to develop alternate designs and applications of the technology. Most of the emphasis was on the assembly and test of a 5 x 5 element module. This effort was supported by evaluation of adhesives and waveguide joint configurations. The evaluation and final assembly considered not only mechanical performance but also producibility in large scale.

Compulsory licensing often did not produce lower prices for antiretrovirals compared to international procurement.

PubMed

Beall, Reed F; Kuhn, Randall; Attaran, Amir

2015-03-01

Compulsory licensing has been widely suggested as a legal mechanism for bypassing patents to introduce lower-cost generic antiretrovirals for HIV/AIDS in developing countries. Previous studies found that compulsory licensing can reduce procurement prices for drugs, but it is unknown how the resulting prices compare to procurements through the Global Fund to Fight AIDS, Tuberculosis, and Malaria; UNICEF; and other international channels. For this study we systematically constructed a case-study database of compulsory licensing activity for antiretrovirals and compared compulsory license prices to those in the World Health Organization's (WHO's) Global Price Reporting Mechanism and the Global Fund's Price and Quality Reporting Tool. Thirty compulsory license cases were analyzed with 673 comparable procurements from WHO and Global Fund data. Compulsory license prices exceeded the median international procurement prices in nineteen of the thirty case studies, often with a price gap of more than 25 percent. Compulsory licensing often delivered suboptimal value when compared to the alternative of international procurement, especially when used by low-income countries to manufacture medicines locally. There is an ongoing need for multilateral and charitable actors to work collectively with governments and medicine suppliers on policy options. Project HOPE—The People-to-People Health Foundation, Inc.

The transformation of the tender evaluation process in public procurement in Poland

NASA Astrophysics Data System (ADS)

Plebankiewicz, E.; Kozik, R.

2017-10-01

Procedures regarding the evaluation of tenders have been changed since the public procurement law was enacted (it came into force in January 1, 1995). The contracting authority could apply both the criteria related to the qualities of the contractor and those related to the to the subject - matter of public contract. Two extensive amendments in 2001 and a government project introduced vital regulations and excluded the possibility of applying criteria related to the qualities of the contractor. Act of 29 January 2004 Public Procurement Law allowed to use price as the sole contract award criterion. The changes in the Law in 2014 restricted that possibility to the situation in which the subject matter of a contract is commonly available and has established quality standards. The Act of 22 June 2016 amending the Public Procurement Law Act and some other laws introduced the new criteria list and limited the importance of the price criterion in the certain situations. Instead of price, the cost can also be a criterion for tender evaluation. The cost criterion can be determined using life cycle costing. In the paper, based on contract notices of open tendering published in the Public Procurement Bulletin, the criteria of construction contract selection will be analysed. In particular the effectiveness of changes in the Procurement Law will be researched.

LSST telescope and site status

NASA Astrophysics Data System (ADS)

Gressler, William J.

2016-07-01

The Large Synoptic Survey Telescope (LSST) Project1 received its construction authorization from the National Science Foundation in August 2014. The Telescope and Site (T and S) group has made considerable progress towards completion in subsystems required to support the scope of the LSST science mission. The LSST goal is to conduct a wide, fast, deep survey via a 3-mirror wide field of view optical design, a 3.2-Gpixel camera, and an automated data processing system. The summit facility is currently under construction on Cerro Pachón in Chile, with major vendor subsystem deliveries and integration planned over the next several years. This paper summarizes the status of the activities of the T and S group, tasked with design, analysis, and construction of the summit and base facilities and infrastructure necessary to control the survey, capture the light, and calibrate the data. All major telescope work package procurements have been awarded to vendors and are in varying stages of design and fabrication maturity and completion. The unique M1M3 primary/tertiary mirror polishing effort is completed and the mirror now resides in storage waiting future testing. Significant progress has been achieved on all the major telescope subsystems including the summit facility, telescope mount assembly, dome, hexapod and rotator systems, coating plant, base facility, and the calibration telescope. In parallel, in-house efforts including the software needed to control the observatory such as the scheduler and the active optics control, have also seen substantial advancement. The progress and status of these subsystems and future LSST plans during this construction phase are presented.

77 FR 34025 - Procurement List; Addition

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-08

..., Engineering Research & Development Center (ERDC), Construction Engineering Research Lab (CERL), 2902 Newmark... W2R2 Const Engrg Lab, Champaign, IL Patricia Briscoe, Deputy Director, Business Operations (Pricing and...

Infrared Thermal Testing Of Mechanical Assemblies At The Military Depot And Field Level: A Progress Report

NASA Astrophysics Data System (ADS)

Kaplan, Herbert

1988-01-01

Based on encouraging results on the Army's programs for infrared mass screening of printed circuit boards at the depot level, the US Army CECOM (Communication-Electronics Command) undertook a one-year investigation of the applicability of similar techniques to screening and diagnostics of mechanical assemblies. These included tanks, helicopters, transport vehicles and their major subassemblies (transmissions, engines, axles, etc.) at field and depot levels. Honeyhill Technical Company was tasked to classify candidate assemblies and perform preliminary measurements using Army-owned general-purpose thermal imaging equipment. The investigations yielded positive results, and it was decided to pursue a comprehensive measurements program using field-mobile equipment specifically procured for the program. This paper summarizes the results of the investigations, outlines the measurements techniques utilized, describes the classification and selection of candidate assemblies, and reports on progress toward the goals of the program.

76 FR 71121 - Notice of Proposed Buy America Waiver

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-16

... seeking a waiver for the procurement of glass panels needed to construct two structural glass pavilions... produced in the United States. 49 CFR 661.7(c)(1). Structural glass is a manufactured product. As such, it.... The proposals included glass panels to be utilized in the construction of two structural glass...

75 FR 16825 - Notice of Modifications to U.S. Commitments Under the World Trade Organization Government...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-02

... hearing- or speech-impairments may access this number through TTY by calling the toll-free Federal... construction project, and the total construction project has an estimated value of more than $7,804,000....S. Commitments Under the World Trade Organization Government Procurement Agreement To Implement...

Sandia National Laboratories: Working with Sandia: Procurement:

Science.gov Websites

Payrolls Before you can submit a timecard you must enter your employees. Please see the Contractor Job Aid link for that specific contract. Please refer to the Contractor Job Aid (MS Word)for further @sandia.gov. Contractor ES&H ES&H for Construction & Service Contracts 01065 Construction Standard

Deployment, release and recovery of ocean riser pipes

DOEpatents

Person, Abraham; Wetmore, Sherman B.; McNary, James F.

1980-11-18

An ocean thermal energy conversion facility includes a long pipe assembly which is supported at its upper end by the hull of the floating facility. Cold water flows to the facility from deep in the ocean. The pipe assembly comprises an elongate pipe construction and a weight connected to the lower end of the construction by a line of selected length. A floatation collar is connected to the construction at its upper end to cause the construction to have positive buoyancy and a center of buoyancy closer to the upper end of the construction than its center of mass. The weight renders the entire pipe assembly negatively buoyant. In the event that support of the pipe assembly should be lost, as by release of the assembly from the facility hull in an emergency, the assembly sinks to the ocean floor where it is moored by the weight. The pipe construction floats submerged above the ocean floor in a substantially vertical attitude which facilitates recovery of the assembly.

7 CFR 3201.68 - Erosion control materials.

Code of Federal Regulations, 2014 CFR

2014-01-01

... MANAGEMENT, DEPARTMENT OF AGRICULTURE GUIDELINES FOR DESIGNATING BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT... manufactured for use on construction, demolition, or other sites to prevent wind or water erosion of loose...

7 CFR 3201.68 - Erosion control materials.

Code of Federal Regulations, 2013 CFR

2013-01-01

... MANAGEMENT, DEPARTMENT OF AGRICULTURE GUIDELINES FOR DESIGNATING BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT... manufactured for use on construction, demolition, or other sites to prevent wind or water erosion of loose...

7 CFR 3201.68 - Erosion control materials.

Code of Federal Regulations, 2012 CFR

2012-01-01

... MANAGEMENT, DEPARTMENT OF AGRICULTURE GUIDELINES FOR DESIGNATING BIOBASED PRODUCTS FOR FEDERAL PROCUREMENT... manufactured for use on construction, demolition, or other sites to prevent wind or water erosion of loose...

Large space telescope engineering scale model optical design

NASA Technical Reports Server (NTRS)

Facey, T. A.

1973-01-01

The objective is to develop the detailed design and tolerance data for the LST engineering scale model optical system. This will enable MSFC to move forward to the optical element procurement phase and also to evaluate tolerances, manufacturing requirements, assembly/checkout procedures, reliability, operational complexity, stability requirements of the structure and thermal system, and the flexibility to change and grow.

Single Day Construction of Multigene Circuits with 3G Assembly.

PubMed

Halleran, Andrew D; Swaminathan, Anandh; Murray, Richard M

2018-05-18

The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting step of the prototyping cycle for synthetic biology. Recently developed DNA assembly methods such as isothermal assembly and type IIS restriction enzyme systems take different approaches to accelerate DNA construction. We introduce a hybrid method, Golden Gate-Gibson (3G), that takes advantage of modular part libraries introduced by type IIS restriction enzyme systems and isothermal assembly's ability to build large DNA constructs in single pot reactions. Our method is highly efficient and rapid, facilitating construction of entire multigene circuits in a single day. Additionally, 3G allows generation of variant libraries enabling efficient screening of different possible circuit constructions. We characterize the efficiency and accuracy of 3G assembly for various construct sizes, and demonstrate 3G by characterizing variants of an inducible cell-lysis circuit.

7 CFR 1726.204 - Multiparty unit price quotations.

Code of Federal Regulations, 2012 CFR

2012-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.204 Multiparty unit price quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.205 - Multiparty lump sum quotations.

Code of Federal Regulations, 2014 CFR

2014-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.205 Multiparty lump sum quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.205 - Multiparty lump sum quotations.

Code of Federal Regulations, 2013 CFR

2013-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.205 Multiparty lump sum quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.204 - Multiparty unit price quotations.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.204 Multiparty unit price quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.204 - Multiparty unit price quotations.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.204 Multiparty unit price quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.205 - Multiparty lump sum quotations.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.205 Multiparty lump sum quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.205 - Multiparty lump sum quotations.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.205 Multiparty lump sum quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.204 - Multiparty unit price quotations.

Code of Federal Regulations, 2013 CFR

2013-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.204 Multiparty unit price quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.205 - Multiparty lump sum quotations.

Code of Federal Regulations, 2012 CFR

2012-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.205 Multiparty lump sum quotations. The borrower or its engineer must contact a sufficient number of...

7 CFR 1726.204 - Multiparty unit price quotations.

Code of Federal Regulations, 2014 CFR

2014-01-01

..., DEPARTMENT OF AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.204 Multiparty unit price quotations. The borrower or its engineer must contact a sufficient number of...

77 FR 22289 - Procurement List Proposed Additions

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-13

...-McChord, WA. Stryker National Logistics Center, Building 2701 C Street SW., Auburn, WA. NPA: Skookum... Center, Construction Engineering Research Lab (ERDC-CERL), 2902 Newmark Drive, Champaign, IL. AT&T...

Construction Quality Assurance Act of 2009

THOMAS, 111th Congress

Rep. Kanjorski, Paul E. [D-PA-11

2009-07-31

House - 10/23/2009 Referred to the Subcommittee on Government Management, Organization, and Procurement. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Organ procurement from executed prisoners in China.

PubMed

Sharif, A; Singh, M Fiatarone; Trey, T; Lavee, J

2014-10-01

Organ procurement from executed prisoners in China is internationally condemned, yet this practice continues unabated in 2014. This is despite repeated announcements from Chinese authorities that constructive measures have been undertaken to conform to accepted ethical standards. While there is unanimous agreement on the unethical nature of using organs from executed prisoners, due to its limitations on voluntary and informed consent, there is insufficient coverage of forced organ procurement from prisoners of conscience without consent. Strategies to influence positive change in China over the last few decades have failed to bring this practice to an end. While organ donation and transplantation services in China have undergone considerable structural changes in the last few years, fundamental attempts to shift practice to ethically sourced organs have floundered. In this article, we discuss the organ trade in China, reflect upon organ procurement from executed prisoners (including both capital prisoners and prisoners of conscience) and provide an overview of contradictory Chinese efforts to halt forced organ procurement from executed prisoners. Finally, we highlight current actions being taken to address this issue and offer comprehensive recommendations to bring this ethically indefensible practice to an immediate end. © Copyright 2014 The American Society of Transplantation and the American Society of Transplant Surgeons.

Eleventh-century shift in timber procurement areas for the great houses of Chaco Canyon

PubMed Central

Guiterman, Christopher H.; Dean, Jeffrey S.

2016-01-01

An enduring mystery from the great houses of Chaco Canyon is the origin of more than 240,000 construction timbers. We evaluate probable timber procurement areas for seven great houses by applying tree-ring width-based sourcing to a set of 170 timbers. To our knowledge, this is the first use of tree rings to assess timber origins in the southwestern United States. We found that the Chuska and Zuni Mountains (>75 km distant) were the most likely sources, accounting for 70% of timbers. Most notably, procurement areas changed through time. Before 1020 Common Era (CE) nearly all timbers originated from the Zunis (a previously unrecognized source), but by 1060 CE the Chuskas eclipsed the Zuni area in total wood imports. This shift occurred at the onset of Chaco florescence in the 11th century, a time with substantial expansion of existing great houses and the addition of seven new great houses in the Chaco Core area. It also coincides with the proliferation of Chuskan stone tools and pottery in the archaeological record of Chaco Canyon, further underscoring the link between land use and occupation in the Chuska area and the peak of great house construction. Our findings, based on the most temporally specific and replicated evidence of Chacoan resource procurement obtained to date, corroborate the long-standing but recently challenged interpretation that large numbers of timbers were harvested and transported from distant mountain ranges to build the great houses at Chaco Canyon. PMID:26644552

Eleventh-century shift in timber procurement areas for the great houses of Chaco Canyon.

PubMed

Guiterman, Christopher H; Swetnam, Thomas W; Dean, Jeffrey S

2016-02-02

An enduring mystery from the great houses of Chaco Canyon is the origin of more than 240,000 construction timbers. We evaluate probable timber procurement areas for seven great houses by applying tree-ring width-based sourcing to a set of 170 timbers. To our knowledge, this is the first use of tree rings to assess timber origins in the southwestern United States. We found that the Chuska and Zuni Mountains (>75 km distant) were the most likely sources, accounting for 70% of timbers. Most notably, procurement areas changed through time. Before 1020 Common Era (CE) nearly all timbers originated from the Zunis (a previously unrecognized source), but by 1060 CE the Chuskas eclipsed the Zuni area in total wood imports. This shift occurred at the onset of Chaco florescence in the 11th century, a time with substantial expansion of existing great houses and the addition of seven new great houses in the Chaco Core area. It also coincides with the proliferation of Chuskan stone tools and pottery in the archaeological record of Chaco Canyon, further underscoring the link between land use and occupation in the Chuska area and the peak of great house construction. Our findings, based on the most temporally specific and replicated evidence of Chacoan resource procurement obtained to date, corroborate the long-standing but recently challenged interpretation that large numbers of timbers were harvested and transported from distant mountain ranges to build the great houses at Chaco Canyon.

Cross-national research on contractor evaluation procedures in public works procurement

NASA Astrophysics Data System (ADS)

Kinoshita, Seiya; Sato, Naoyoshi; Matsumoto, Naoya

Contractor evaluation methods in Japan's public works procurement, beginning with construction business licensure, going through biennial preliminary firm rating, up to project-by-project prequalification and comprehensive point rating, were developed during the period when public works were mostly procured through designated competitive bidding. It is essential to focus attention on contractor evaluation methods for introducing different types of procurement procedures which enhance the use of technological capabilities held by private businesses. An overall review of contractor evaluation procedures should be conducted in view of the present situation, where the open competitive bidding has become mainly used in combination with comprehensive evaluation, as well as to allow for further diversification of procurement methods. In Western countries, improvements have been made for the past several years in contractor evaluation procedures with more emphasis on "Value for Money." Advanced efforts made by these countries will be useful as a reference for overhauling Japan's contractor evaluation system. This study conducts a comparative review of contractor evaluation procedures for public procurement in Western countries such as the United States, the United Kingdom and France by identifying similarities and differences between those of Japan and the above mentioned countries. This reveals that a contractor's technical or professional ability is looked at separately from its economic and financial standing in those countries studied, and there is no case like Japan in which those two factors are integrated into one for evaluation.

78 FR 18348 - Submission for OMB Review; Use of Project Labor Agreements for Federal Construction Projects

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-26

... agreement (PLA), as they may decide appropriate, on large-scale construction projects, where the total cost... procurement. A PLA is a pre-hire collective bargaining agreement with one or more labor organizations that... the use of a project labor agreement (PLA), as they may decide appropriate, on large-scale...

40 CFR 33.301 - What does this subpart require?

Code of Federal Regulations, 2014 CFR

2014-07-01

... AGENCY PROGRAMS Good Faith Efforts § 33.301 What does this subpart require? A recipient, including one... good faith efforts whenever procuring construction, equipment, services and supplies under an EPA...

40 CFR 33.301 - What does this subpart require?

Code of Federal Regulations, 2013 CFR

2013-07-01

... AGENCY PROGRAMS Good Faith Efforts § 33.301 What does this subpart require? A recipient, including one... good faith efforts whenever procuring construction, equipment, services and supplies under an EPA...

40 CFR 33.301 - What does this subpart require?

Code of Federal Regulations, 2012 CFR

2012-07-01

... AGENCY PROGRAMS Good Faith Efforts § 33.301 What does this subpart require? A recipient, including one... good faith efforts whenever procuring construction, equipment, services and supplies under an EPA...

40 CFR 33.301 - What does this subpart require?

Code of Federal Regulations, 2011 CFR

2011-07-01

... AGENCY PROGRAMS Good Faith Efforts § 33.301 What does this subpart require? A recipient, including one... good faith efforts whenever procuring construction, equipment, services and supplies under an EPA...

7 CFR 1942.126 - Planning, bidding, contracting, constructing, procuring.

Code of Federal Regulations, 2013 CFR

2013-01-01

... evaluate the proposals received or can obtain adequate assistance from other sources, such as State or.... Contract documents must be sufficiently descriptive and legally binding to accomplish the work as...

7 CFR 1942.126 - Planning, bidding, contracting, constructing, procuring.

Code of Federal Regulations, 2012 CFR

2012-01-01

... evaluate the proposals received or can obtain adequate assistance from other sources, such as State or.... Contract documents must be sufficiently descriptive and legally binding to accomplish the work as...

7 CFR 1942.126 - Planning, bidding, contracting, constructing, procuring.

Code of Federal Regulations, 2010 CFR

2010-01-01

... evaluate the proposals received or can obtain adequate assistance from other sources, such as State or.... Contract documents must be sufficiently descriptive and legally binding to accomplish the work as...

7 CFR 1942.126 - Planning, bidding, contracting, constructing, procuring.

Code of Federal Regulations, 2011 CFR

2011-01-01

... evaluate the proposals received or can obtain adequate assistance from other sources, such as State or.... Contract documents must be sufficiently descriptive and legally binding to accomplish the work as...

7 CFR 1942.126 - Planning, bidding, contracting, constructing, procuring.

Code of Federal Regulations, 2014 CFR

2014-01-01

... evaluate the proposals received or can obtain adequate assistance from other sources, such as State or.... Contract documents must be sufficiently descriptive and legally binding to accomplish the work as...

29 CFR 1926.1403 - Assembly/Disassembly-selection of manufacturer or employer procedures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 8 2013-07-01 2013-07-01 false Assembly/Disassembly-selection of manufacturer or employer... CONSTRUCTION Cranes and Derricks in Construction § 1926.1403 Assembly/Disassembly—selection of manufacturer or... applicable to assembly and disassembly, or (b) Employer procedures for assembly and disassembly. Employer...

29 CFR 1926.1403 - Assembly/Disassembly-selection of manufacturer or employer procedures.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 8 2012-07-01 2012-07-01 false Assembly/Disassembly-selection of manufacturer or employer... CONSTRUCTION Cranes and Derricks in Construction § 1926.1403 Assembly/Disassembly—selection of manufacturer or... applicable to assembly and disassembly, or (b) Employer procedures for assembly and disassembly. Employer...

29 CFR 1926.1403 - Assembly/Disassembly-selection of manufacturer or employer procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 8 2014-07-01 2014-07-01 false Assembly/Disassembly-selection of manufacturer or employer... CONSTRUCTION Cranes and Derricks in Construction § 1926.1403 Assembly/Disassembly—selection of manufacturer or... applicable to assembly and disassembly, or (b) Employer procedures for assembly and disassembly. Employer...

29 CFR 1926.1403 - Assembly/Disassembly-selection of manufacturer or employer procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 8 2011-07-01 2011-07-01 false Assembly/Disassembly-selection of manufacturer or employer... CONSTRUCTION Cranes and Derricks in Construction § 1926.1403 Assembly/Disassembly—selection of manufacturer or... applicable to assembly and disassembly, or (b) Employer procedures for assembly and disassembly. Employer...

United States Special Operations Command (USSOCOM): Construction Management Handbook

DTIC Science & Technology

2010-02-11

installation B-Hut Road Work Sea-Hut Water Well and Pump House Brick and Mortar billets D. Oversight Program Oversight of contracts is critical to the...often asked to fulfill construction requirements in austere locations with little or no training in construction terminology, concepts, and protocols...contracting arm of SOAL is the Director of Procurement (also known as SOAL-K), located within the USSOCOM Headquarters on MacDill AFB, Florida. SOAL-K

Stellar Inertial Navigation Workstation

NASA Technical Reports Server (NTRS)

Johnson, W.; Johnson, B.; Swaminathan, N.

1989-01-01

Software and hardware assembled to support specific engineering activities. Stellar Inertial Navigation Workstation (SINW) is integrated computer workstation providing systems and engineering support functions for Space Shuttle guidance and navigation-system logistics, repair, and procurement activities. Consists of personal-computer hardware, packaged software, and custom software integrated together into user-friendly, menu-driven system. Designed to operate on IBM PC XT. Applied in business and industry to develop similar workstations.

Construction Quality Assurance Act of 2011

THOMAS, 112th Congress

Rep. Maloney, Carolyn B. [D-NY-14

2011-05-05

House - 05/13/2011 Referred to the Subcommittee on Technology, Information Policy, Intergovernmental Relations and Procurement Reform . (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

QA program plan plutonium stabilization and handling project W-460

DOE Office of Scientific and Technical Information (OSTI.GOV)

SCHULTZ, J.W.

This Quality Assurance Program Plan (QAPP) identifies Project Quality Assurance (QA) program requirements for all parties participating in the design, procurement, demolition, construction, installation, inspection and testing for Project W-460.

In-Space Assembly and Construction Technology Project Summary: Infrastructure Operations Area of the Operations Technology Program

NASA Technical Reports Server (NTRS)

Bush, Harold

1991-01-01

Viewgraphs describing the in-space assembly and construction technology project of the infrastructure operations area of the operation technology program are presented. Th objective of the project is to develop and demonstrate an in-space assembly and construction capability for large and/or massive spacecraft. The in-space assembly and construction technology program will support the need to build, in orbit, the full range of spacecraft required for the missions to and from planet Earth, including: earth-orbiting platforms, lunar transfer vehicles, and Mars transfer vehicles.

Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Torella, JP; Boehm, CR; Lienert, F

2013-12-28

In vitro recombination methods have enabled one-step construction of large DNA sequences from multiple parts. Although synthetic biological circuits can in principle be assembled in the same fashion, they typically contain repeated sequence elements such as standard promoters and terminators that interfere with homologous recombination. Here we use a computational approach to design synthetic, biologically inactive unique nucleotide sequences (UNSes) that facilitate accurate ordered assembly. Importantly, our designed UNSes make it possible to assemble parts with repeated terminator and insulator sequences, and thereby create insulated functional genetic circuits in bacteria and mammalian cells. Using UNS-guided assembly to construct repeating promoter-gene-terminatormore » parts, we systematically varied gene expression to optimize production of a deoxychromoviridans biosynthetic pathway in Escherichia coli. We then used this system to construct complex eukaryotic AND-logic gates for genomic integration into embryonic stem cells. Construction was performed by using a standardized series of UNS-bearing BioBrick-compatible vectors, which enable modular assembly and facilitate reuse of individual parts. UNS-guided isothermal assembly is broadly applicable to the construction and optimization of genetic circuits and particularly those requiring tight insulation, such as complex biosynthetic pathways, sensors, counters and logic gates.« less

Professional services contract manager development and certification strategy : spr 696 : final report.

DOT National Transportation Integrated Search

2013-02-27

SCDOT hires many consultants to provide professional services in support of its planning, design, construction and : maintenance projects. SCDOT personnel responsible for procuring and administering these professional service : contracts, especially ...

Evaluation of the towplow for Caltrans operations.

DOT National Transportation Integrated Search

2015-09-30

Caltrans requested that the Advanced Highway Maintenance and Construction Technology Research Center (AHMCT) research center configure, procure, and deploy two Viking-Cives TowPlow systems and conduct an extensive evaluation to determine the most ben...

40 CFR 35.936 - Procurement.

Code of Federal Regulations, 2014 CFR

2014-07-01

... engineering services as defined in § 35.937 and construction contracts as described in § 35.938 by grantees... does not apply to work beyond the scope of the project for which grant assistance is awarded (i.e...

40 CFR 35.936 - Procurement.

Code of Federal Regulations, 2011 CFR

2011-07-01

... engineering services as defined in § 35.937 and construction contracts as described in § 35.938 by grantees... does not apply to work beyond the scope of the project for which grant assistance is awarded (i.e...

40 CFR 35.936 - Procurement.

Code of Federal Regulations, 2013 CFR

2013-07-01

... engineering services as defined in § 35.937 and construction contracts as described in § 35.938 by grantees... does not apply to work beyond the scope of the project for which grant assistance is awarded (i.e...

Risk Analysis of Underestimate Cost Offer to The Project Quality in Aceh Province

NASA Astrophysics Data System (ADS)

Rani, Hafnidar A.

2016-11-01

The possibility of errors in the process of offer price determination could be enormous, so it can affect the possibility of project underestimate cost which can impact and reduce the profit if being implementing. Government Equipment/Service Procurement Policy Institution (LKPP) assesses that the practices of cheaper price in the government equipment/service procurement are still highly found and can be potential to decrease the project quality. This study aimed to analyze the most dominant factors happened in underestimate cost offer practice, to analyze the relationship of underestimate cost offer risk factors to road construction project quality in Aceh Province and to analyze the most potential factors of underestimate cost offer risk affecting road construction project quality in Aceh Province. Road construction projects observed the projects which have been implemented in Aceh Province since 2013 - 2015. This study conducted by interviewing Government Budget Authority (KPA), and distributing the questionnaire to the road construction contractors with the qualification of K1, K2, K3, M1, M2 and B1. Based on the data from Construction Service Development Institution (LPJK) of Aceh Province on 2016, the populations obtained are 2,717 constructors. By using Slovin Equation, the research samples obtained are 97 contractors. The most dominant factors in underestimate cost offer risk of the road construction projects in Aceh Province is Contingency Cost Factor which the mean is 4.374.

29 CFR 1926.1406 - Assembly/Disassembly-employer procedures-general requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 8 2012-07-01 2012-07-01 false Assembly/Disassembly-employer procedures-general... CONSTRUCTION Cranes and Derricks in Construction § 1926.1406 Assembly/Disassembly—employer procedures—general requirements. (a) When using employer procedures instead of manufacturer procedures for assembly/disassembly...

29 CFR 1926.1406 - Assembly/Disassembly-employer procedures-general requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 8 2014-07-01 2014-07-01 false Assembly/Disassembly-employer procedures-general... CONSTRUCTION Cranes and Derricks in Construction § 1926.1406 Assembly/Disassembly—employer procedures—general requirements. (a) When using employer procedures instead of manufacturer procedures for assembly/disassembly...

29 CFR 1926.1406 - Assembly/Disassembly-employer procedures-general requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 8 2013-07-01 2013-07-01 false Assembly/Disassembly-employer procedures-general... CONSTRUCTION Cranes and Derricks in Construction § 1926.1406 Assembly/Disassembly—employer procedures—general requirements. (a) When using employer procedures instead of manufacturer procedures for assembly/disassembly...

29 CFR 1926.1406 - Assembly/Disassembly-employer procedures-general requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 8 2011-07-01 2011-07-01 false Assembly/Disassembly-employer procedures-general... CONSTRUCTION Cranes and Derricks in Construction § 1926.1406 Assembly/Disassembly—employer procedures—general requirements. (a) When using employer procedures instead of manufacturer procedures for assembly/disassembly...

A puzzle assembly strategy for fabrication of large engineered cartilage tissue constructs.

PubMed

Nover, Adam B; Jones, Brian K; Yu, William T; Donovan, Daniel S; Podolnick, Jeremy D; Cook, James L; Ateshian, Gerard A; Hung, Clark T

2016-03-21

Engineering of large articular cartilage tissue constructs remains a challenge as tissue growth is limited by nutrient diffusion. Here, a novel strategy is investigated, generating large constructs through the assembly of individually cultured, interlocking, smaller puzzle-shaped subunits. These constructs can be engineered consistently with more desirable mechanical and biochemical properties than larger constructs (~4-fold greater Young׳s modulus). A failure testing technique was developed to evaluate the physiologic functionality of constructs, which were cultured as individual subunits for 28 days, then assembled and cultured for an additional 21-35 days. Assembled puzzle constructs withstood large deformations (40-50% compressive strain) prior to failure. Their ability to withstand physiologic loads may be enhanced by increases in subunit strength and assembled culture time. A nude mouse model was utilized to show biocompatibility and fusion of assembled puzzle pieces in vivo. Overall, the technique offers a novel, effective approach to scaling up engineered tissues and may be combined with other techniques and/or applied to the engineering of other tissues. Future studies will aim to optimize this system in an effort to engineer and integrate robust subunits to fill large defects. Copyright © 2016 Elsevier Ltd. All rights reserved.

A Puzzle Assembly Strategy for Fabrication of Large Engineered Cartilage Tissue Constructs

PubMed Central

Nover, Adam B.; Jones, Brian K.; Yu, William T.; Donovan, Daniel S.; Podolnick, Jeremy D.; Cook, James L.; Ateshian, Gerard A.; Hung, Clark T.

2016-01-01

Engineering of large articular cartilage tissue constructs remains a challenge as tissue growth is limited by nutrient diffusion. Here, a novel strategy is investigated, generating large constructs through the assembly of individually cultured, interlocking, smaller puzzle-shaped subunits. These constructs can be engineered consistently with more desirable mechanical and biochemical properties than larger constructs (~4-fold greater Young's modulus). A failure testing technique was developed to evaluate the physiologic functionality of constructs, which were cultured as individual subunits for 28 days, then assembled and cultured for an additional 21-35 days. Assembled puzzle constructs withstood large deformations (40-50% compressive strain) prior to failure. Their ability to withstand physiologic loads may be enhanced by increases in subunit strength and assembled culture time. A nude mouse model was utilized to show biocompatibility and fusion of assembled puzzle pieces in vivo. Overall, the technique offers a novel, effective approach to scaling up engineered tissues and may be combined with other techniques and/or applied to the engineering of other tissues. Future studies will aim to optimize this system in an effort to engineer and integrate robust subunits to fill large defects. PMID:26895780

Microfluidic assembly blocks.

PubMed

Rhee, Minsoung; Burns, Mark A

2008-08-01

An assembly approach for microdevice construction using prefabricated microfluidic components is presented. Although microfluidic systems are convenient platforms for biological assays, their use in the life sciences is still limited mainly due to the high-level fabrication expertise required for construction. This approach involves prefabrication of individual microfluidic assembly blocks (MABs) in PDMS that can be readily assembled to form microfluidic systems. Non-expert users can assemble the blocks on glass slides to build their devices in minutes without any fabrication steps. In this paper, we describe the construction and assembly of the devices using the MAB methodology, and demonstrate common microfluidic applications including laminar flow development, valve control, and cell culture.

Construction method of pre assembled unit of bolt sphere grid

NASA Astrophysics Data System (ADS)

Hu, L. W.; Guo, F. L.; Wang, J. L.; Bu, F. M.

2018-03-01

The traditional construction of bolt sphere grid has many disadvantages, such as high cost, large amount of work at high altitude and long construction period, in order to make up for these shortcomings, in this paper, a new and applicable construction method is explored: setting up local scaffolding, installing the bolt sphere grid starting frame on the local scaffolding, then the pre assembled unit of bolt sphere grid is assembled on the ground, using small hoisting equipment to lift pre assembled unit to high altitude and install. Compared with the traditional installation method, the construction method has strong practicability and high economic efficiency, and has achieved good social and economic benefits.

40 CFR 40.145-3 - Projects involving construction.

Code of Federal Regulations, 2010 CFR

2010-07-01

... competition. “Brand name or equal” description may be used as a means to define the performance or other salient requirements of a procurement, and when so used the specific features of the named brand which...

A space crane concept for performing on-orbit assembly

NASA Technical Reports Server (NTRS)

Dorsey, John T.

1992-01-01

The topics are presented in viewgraph form and include: in-space assembly and construction enhances future mission planning flexibility; in-space assembly and construction facility concept; space crane concept with mobile base; fundamental characteristics; space crane research approach; spacecraft component positioning and assembly test-bed; and articulating joint testbed.

Capability 9.3 Assembly and Deployment

NASA Technical Reports Server (NTRS)

Dorsey, John

2005-01-01

Large space systems are required for a range of operational, commercial and scientific missions objectives however, current launch vehicle capacities substantially limit the size of space systems (on-orbit or planetary). Assembly and Deployment is the process of constructing a spacecraft or system from modules which may in turn have been constructed from sub-modules in a hierarchical fashion. In-situ assembly of space exploration vehicles and systems will require a broad range of operational capabilities, including: Component transfer and storage, fluid handling, construction and assembly, test and verification. Efficient execution of these functions will require supporting infrastructure, that can: Receive, store and protect (materials, components, etc.); hold and secure; position, align and control; deploy; connect/disconnect; construct; join; assemble/disassemble; dock/undock; and mate/demate.

A Study on Market-based Strategic Procurement Planning in Convergent Supply Networks

NASA Astrophysics Data System (ADS)

Opadiji, Jayeola Femi; Kaihara, Toshiya

We present a market-based decentralized approach which uses a market-oriented programming algorithm to obtain Pareto-optimal allocation of resources traded among agents which represent enterprise units in a supply network. The proposed method divides the network into a series of Walrsian markets in order to obtain procurement budgets for enterprises in the network. An interaction protocol based on market value propagation is constructed to coordinate the flow of resources across the network layers. The method mitigates the effect of product complementarity in convergent network by allowing for enterprises to hold private valuations of resources in the markets.

Lunar surface construction and assembly equipment study: Lunar Base Systems Study (LBSS) task 5.3

NASA Technical Reports Server (NTRS)

1988-01-01

A set of construction and assembly tasks required on the lunar surface was developed, different concepts for equipment applicable to the tasks determined, and leading candidate systems identified for future conceptual design. Data on surface construction and assembly equipment systems are necessary to facilitate an integrated review of a complete lunar scenario.

Moving Equipment and Workers to Mine Construction Site at a Logistically Challenged Area

NASA Astrophysics Data System (ADS)

Tikasz, Laszlo; Biroscak, Dennis; Pentiah, Scheale Duvah; McCulloch, Robert I.

Social sensitivity of habitants, minimal impact on the environment, low-grade infrastructure, high altitude, frequent rock slides combined with expectations for the timely moving of equipment and workers are some of the challenges emerging from the current construction of a mine. Starting with traditional planning, and experiencing issues in the early phase of the construction, a traffic simulator was requested by the Procurement Department in order to validate daily-weekly schedules and predict likely delays or blockages on the long-term.

Photovoltaic balance-of-system designs and costs at PVUSA

NASA Astrophysics Data System (ADS)

Reyes, A. B.; Jennings, C.

1995-05-01

This report is one in a series of 1994-1995 PVUSA reports that document PVUSA lessons learned at demonstration sites in California and Texas. During the last 7 years (1988 to 1994), 16 PV systems ranging from 20 kW to 500 kW have been installed. Six 20-kW emerging module technology (EMT) arrays and three turnkey (i.e., vendor designed and integrated) utility-scale systems were procured and installed at PVUSA's main test site in Davis, California. PVUSA host utilities have installed a total of seven EMT arrays and utility-scale systems in their service areas. Additional systems at Davis and host utility sites are planned. One of PVUSA's key objectives is to evaluate the performance, reliability, and cost of PV balance-of-system (BOS). In the procurement stage PVUSA encouraged innovative design to improve upon present practice by reducing maintenance, improving reliability, or lowering manufacturing or construction costs. The project team worked closely with suppliers during the design stage not only to ensure designs met functional and safety specifications, but to provide suggestions for improvement. This report, intended for the photovoltaic (PV) industry and for utility project managers and engineers considering PV plant construction and ownership, documents PVUSA utility-scale system design and cost lessons learned. Complementary PVUSA topical reports document: construction and safety experience; five-year assessment of EMTs; validation of the Kerman 500-kW grid-support PV plant benefits; PVUSA instrumentation and data analysis techniques; procurement, acceptance, and rating practices for PV power plants; experience with power conditioning units and power quality.

Procurement of Architectural and Engineering Services for Sustainable Buildings: A Guide for Federal Project Managers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

2004-06-01

This guide was prepared to be a resource for federal construction project managers and others who want to integrate the principles of sustainable design into the procurement of professional building design and consulting services. To economize on energy costs and improve the safety, comfort, and health of building occupants, building design teams can incorporate daylighting, energy efficiency, renewable energy, and passive solar design into all projects in which these elements are technically and economically feasible. The information presented here will help project leaders begin the process and manage the inclusion of sustainable design in the procurement process. The section onmore » establishing selection criteria contains key elements to consider before selecting an architectural and engineering (A/E) firm. The section on preparing the statement of work discusses the broad spectrum of sustainable design services that an A/E firm can provide. Several helpful checklists are included.« less

Assembly of Customized TAL Effectors Through Advanced ULtiMATE System.

PubMed

Yang, Junjiao; Guo, Shengjie; Yuan, Pengfei; Wei, Wensheng

2016-01-01

Transcription activator-like effectors (TALEs) have been widely applied in gene targeting. Here we describe an advanced ULtiMATE (USER-based Ligation-Mediated Assembly of TAL Effector) system that utilizes USER fusion technique and archive of 512 tetramer templates to achieve highly efficient construction of TALEs, which takes only half a day to accomplish the assembly of any given TALE construct. This system is also suitable for large-scale assembly of TALENs and any other TALE-based constructions.

Procurement Opportunitites in: Afghanistan and the Central Asian States (CAS)

DTIC Science & Technology

2012-08-01

barriers) Furniture (office and household) Prefabricated Structures (CONEXs, containerized housing units, re-locatable buildings) Office Supplies... Concrete Foundation and Structure Road Support Activities Minor Building Renovation Water and Sewer Line and Related Construction Heavy and Civil

Minority Business Enterprises and Woman Business Enterprises Grant Utilization

EPA Pesticide Factsheets

The policy goal of the MBE/WBE Programs is to assure that minority business enterprises and woman business enterprises are given the opportunity to participate in contract and procurement for supplies, construction, equipment & services under any EPA grant

Research on the Development Prospect of Assembled Passive Building Based on Green Development Concept

NASA Astrophysics Data System (ADS)

Lixin, Zhang; Ju, Ma; Baohui, He

2018-02-01

In recent years, the vigorous development of the construction industry has brought about serious problems of environmental pollution and resource consumption. In order to reduce the negative impact that the construction industry has on the natural environment,this paper, from the perspective of environmental protection, studies the pollution and high consumption problems existing in the production and use of traditional construction industry, compares and analyzes the green and energy-saving advantages in the construction and using phase of assembled passive building, and at the same time, combined with our country is vigorously promoting the assembled passive building and the green development, concluded that the assembled passive building is the new development direction of China’s construction industry.

Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches

PubMed Central

Shi, Zhenyu; Wedd, Anthony G.; Gras, Sally L.

2013-01-01

The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration. PMID:23468883

Assembling of Steel Angle Headframe of Multifunctional Purpose

NASA Astrophysics Data System (ADS)

Kassikhina, E. G.; Pershin, V. V.; Volkov, V. M.

2017-10-01

The article reviews new technical solution on equipment provision of vertical shaft utilizing steel headframe of multifunctional purpose. Practice of construction of coal and ore mines provides application of various designs for steel angle headframes which are divided into separate large assembly blocks and constructive elements during assembling operations. Design of these blocks and elements, their weight and dimensions effect the chose of the method of assembling on which economic and technological indicators, as well as duration of down-time, depend on during performance of construction operations in shaft. The technical solution on equipment provision for mine vertical shaft using headframe of multifunctional purpose will allow changing the management construction of vertical shaft. The proposed headframe combines the functions of sinking and operation that eliminates costs for assembling/dissembling of temporary headgear. The constructive design of the headgear allows application of the effective method of assembly and thus to provide improvement of the technical and economic indexes, and high calendar time rate of the shaft construction due to reduction of duration of works on equipment provision for the shaft and to refurbishment of the shaft in order to carry out horizontal mining.

Synthetic approaches to construct viral capsid-like spherical nanomaterials.

PubMed

Matsuura, Kazunori

2018-06-06

This feature article describes recent progress in synthetic strategies to construct viral capsid-like spherical nanomaterials using the self-assembly of peptides and/or proteins. By mimicking the self-assembly of spherical viral capsids and clathrin, trigonal peptide conjugates bearing β-sheet-forming peptides, glutathiones, or coiled-coil-forming peptides were developed to construct viral capsid-like particles. β-Annulus peptides from tomato bushy stunt virus self-assembled into viral capsid-like nanocapsules with a size of 30-50 nm, which could encapsulate various guest molecules and be decorated with different molecules on their surface. Rationally designed fusion proteins bearing symmetric assembling units afforded precise viral capsid-like polyhedral assemblies. These synthetic approaches to construct artificial viruses could become useful guidelines to develop novel drug carriers, vaccine platforms, nanotemplates and nanoreactors.

Micro-Masonry: Construction of 3D Structures by Mesoscale Self-Assembly

PubMed Central

Fernandez, Javier G.; Khademhosseini, Ali

2010-01-01

A general method for construction of three dimensional structures by directed assembly of microscale polymeric sub-units is presented. Shape-controlled microgels are directed to assemble into different shapes by limiting their movement onto a molded substrate. The capillary forces, resulting from the presence of a liquid polymer, assemble the microgels in close contact with the rest of the units and with the free surface, the latter imposing the final geometry of the resulting construct. The result is a freestanding structure composed of one or multiple layers of sub-units assembled in a tightly packed conformation. The applicability of the technique for the construction of scaffolds with cell-laden sub-units is demonstrated. In addition, scaffolds formed by the sequential aggregation of sub-units are produced. PMID:20440697

The versatility of a truss mounted mobile transporter for in-space construction

NASA Technical Reports Server (NTRS)

Bush, Harold G.; Lake, Mark S.; Watson, Judith J.; Heard, Walter L., Jr.

1988-01-01

The Mobile Transporter (MT) evolution from early erectable structures assembly activities is detailed. The MT operational features which are required to support astronauts performing on-orbit structure construction or spacecraft assembly functions are presented and discussed. Use of the MT to perform a variety of assembly functions is presented. Estimated EVA assembly times for a precision segmented reflector approximately 20 m in diameter are presented. The EVA/MT technique under study for construction of the reflector (and the entire spacecraft) is illustrated. Finally, the current status of development activities and test results involving the MT and Space Station structural assembly are presented.

7 CFR 1724.70 - Standard forms of contracts for borrowers.

Code of Federal Regulations, 2010 CFR

2010-01-01

... SERVICE, DEPARTMENT OF AGRICULTURE ELECTRIC ENGINEERING, ARCHITECTURAL SERVICES AND DESIGN POLICIES AND... construction, procurement, engineering services, and architectural services financed by a loan made or... prescribes RUS procedures in promulgating electric program standard contract forms and identifies those forms...

77 FR 69627 - Information Collection; Use of Project Labor Agreements for Federal Construction Projects

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-20

... agencies to consider the use of a project labor agreement (PLA), as they may decide appropriate, on large... to promote economy and efficiency in Federal procurement. A PLA is a pre-hire collective bargaining...

Construction and engineering of large biochemical pathways via DNA assembler

PubMed Central

Shao, Zengyi; Zhao, Huimin

2015-01-01

Summary DNA assembler enables rapid construction and engineering of biochemical pathways in a one-step fashion by exploitation of the in vivo homologous recombination mechanism in Saccharomyces cerevisiae. It has many applications in pathway engineering, metabolic engineering, combinatorial biology, and synthetic biology. Here we use two examples including the zeaxanthin biosynthetic pathway and the aureothin biosynthetic gene cluster to describe the key steps in the construction of pathways containing multiple genes using the DNA assembler approach. Methods for construct design, pathway assembly, pathway confirmation, and functional analysis are shown. The protocol for fine genetic modifications such as site-directed mutagenesis for engineering the aureothin gene cluster is also illustrated. PMID:23996442

Supramolecular Differentiation for Construction of Anisotropic Fullerene Nanostructures by Time-Programmed Control of Interfacial Growth.

PubMed

Bairi, Partha; Minami, Kosuke; Hill, Jonathan P; Nakanishi, Waka; Shrestha, Lok Kumar; Liu, Chao; Harano, Koji; Nakamura, Eiichi; Ariga, Katsuhiko

2016-09-27

Supramolecular assembly can be used to construct a wide variety of ordered structures by exploiting the cumulative effects of multiple noncovalent interactions. However, the construction of anisotropic nanostructures remains subject to some limitations. Here, we demonstrate the preparation of anisotropic fullerene-based nanostructures by supramolecular differentiation, which is the programmed control of multiple assembly strategies. We have carefully combined interfacial assembly and local phase separation phenomena. Two fullerene derivatives, PhH and C12H, were together formed into self-assembled anisotropic nanostructures by using this approach. This technique is applicable for the construction of anisotropic nanostructures without requiring complex molecular design or complicated methodology.

[Self-assembly tissue engineering fibrocartilage model of goat temporomandibular joint disc].

PubMed

Kang, Hong; Li, Zhen-Qiang; Bi, Yan-Da

2011-06-01

To construct self-assembly fibrocartilage model of goat temporomandibular joint disc and observe the biological characteristics of the self-assembled fibrocartilage constructs, further to provide a basis for tissue engineering of the temporomandibular joint disc and other fibrocartilage. Cells from temporomandibular joint discs of goats were harvested and cultured. 5.5 x 10(6) cells were seeded in each agarose well with diameter 5 mm x depth 10 mm, daily replace of medium, cultured for 2 weeks. One day after seeding, goat temporomandibular joint disc cells in agarose wells were gathered and began to self-assemble into a disc-shaped base, then gradually turned into a round shape. When cultured for 2 weeks, hematoxylin-eosin staining was conducted and observed that cells were round and wrapped around by the matrix. Positive Safranin-O/fast green staining for glycosaminoglycans was observed throughout the entire constructs, and picro-sirius red staining was examined and distribution of numerous type I collagen was found. Immunohistochemistry staining demonstrated brown yellow particles in cytoplasm and around extracellular matrix, which showed self-assembly construct can produce type I collagen as native temporomandibular joint disc tissue. Production of extracellular matrix in self-assembly construct as native temporomandibular joint disc tissue indicates that the use of agarose wells to construct engineered temporomandibular joint disc will be possible and practicable.

General Services Administration Streamlines the Procurement of Construction Services

DTIC Science & Technology

2010-05-01

successful. The system must be changed to increase performance, and have a sustainable performance measurement system that results in an increase...currently employ, and what is needed to employ a sustainable performance measurement system that has accompanying increase in performance and value

7 CFR 1726.201 - Formal competitive bidding.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.201 Formal... formal competitive bidding: (a) Selection of qualified bidders. The borrower (acting through its engineer... § 1726.23). (b) Invitations to bid. The borrower (acting through its engineer, if applicable) is...

7 CFR 1726.201 - Formal competitive bidding.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.201 Formal... formal competitive bidding: (a) Selection of qualified bidders. The borrower (acting through its engineer... § 1726.23). (b) Invitations to bid. The borrower (acting through its engineer, if applicable) is...

7 CFR 1726.201 - Formal competitive bidding.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.201 Formal... formal competitive bidding: (a) Selection of qualified bidders. The borrower (acting through its engineer... § 1726.23). (b) Invitations to bid. The borrower (acting through its engineer, if applicable) is...

7 CFR 1726.201 - Formal competitive bidding.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.201 Formal... formal competitive bidding: (a) Selection of qualified bidders. The borrower (acting through its engineer... § 1726.23). (b) Invitations to bid. The borrower (acting through its engineer, if applicable) is...

7 CFR 1726.201 - Formal competitive bidding.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES Procurement Procedures § 1726.201 Formal... formal competitive bidding: (a) Selection of qualified bidders. The borrower (acting through its engineer... § 1726.23). (b) Invitations to bid. The borrower (acting through its engineer, if applicable) is...

7 CFR 1753.1 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

... unacceptable loan security risks to RUS. (4) The P&S or contract have been modified. (e) The requirements and... Telecommunications Loan Documents contain provisions regarding procurement of materials and equipment and... and procedures whenever using loan funds to purchase materials and equipment or perform construction...

7 CFR 1755.27 - Borrower contractual obligations.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., DEPARTMENT OF AGRICULTURE TELECOMMUNICATIONS POLICIES ON SPECIFICATIONS, ACCEPTABLE MATERIALS, AND STANDARD CONTRACT FORMS § 1755.27 Borrower contractual obligations. (a) Loan agreement. As a condition of a loan or... pursuant to which the borrowers agree to use RUS standard contract forms for construction, procurement...

BASIC: A Simple and Accurate Modular DNA Assembly Method.

PubMed

Storch, Marko; Casini, Arturo; Mackrow, Ben; Ellis, Tom; Baldwin, Geoff S

2017-01-01

Biopart Assembly Standard for Idempotent Cloning (BASIC) is a simple, accurate, and robust DNA assembly method. The method is based on linker-mediated DNA assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts [1]. The BASIC standard defines a single entry vector for all parts flanked by the same prefix and suffix sequences and its idempotent nature means that the assembled construct is returned in the same format. Once a part has been adapted into the BASIC format it can be placed at any position within a BASIC assembly without the need for reformatting. This allows laboratories to grow comprehensive and universal part libraries and to share them efficiently. The modularity within the BASIC framework is further extended by the possibility of encoding ribosomal binding sites (RBS) and peptide linker sequences directly on the linkers used for assembly. This makes BASIC a highly versatile library construction method for combinatorial part assembly including the construction of promoter, RBS, gene variant, and protein-tag libraries. In comparison with other DNA assembly standards and methods, BASIC offers a simple robust protocol; it relies on a single entry vector, provides for easy hierarchical assembly, and is highly accurate for up to seven parts per assembly round [2].

Columbia Quilt

NASA Image and Video Library

2018-02-22

A certificate is on display that confirms the transfer of a giant hand-made quilt in honor of space shuttle Columbia and her crew from the Office of Procurement to the Columbia Preservation Room inside the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida. The quilt was made by Katherine Walsh, a lifelong NASA and space program fan originally from Kentucky. The quilt will be displayed with its certificate in the preservation room as part of NASA's Apollo, Challenger, Columbia Lessons Learned Program.

Low-carbon building assessment and multi-scale input-output analysis

NASA Astrophysics Data System (ADS)

Chen, G. Q.; Chen, H.; Chen, Z. M.; Zhang, Bo; Shao, L.; Guo, S.; Zhou, S. Y.; Jiang, M. M.

2011-01-01

Presented as a low-carbon building evaluation framework in this paper are detailed carbon emission account procedures for the life cycle of buildings in terms of nine stages as building construction, fitment, outdoor facility construction, transportation, operation, waste treatment, property management, demolition, and disposal for buildings, supported by integrated carbon intensity databases based on multi-scale input-output analysis, essential for low-carbon planning, procurement and supply chain design, and logistics management.

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2010-06-10

Background, Issues, and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK...out of sequence and significant rework has been required, disrupting the optimal construction sequence and application of lessons learned for...concerned about Northrop Grumman Ship Systems’ ( NGSS ) ability to recover in the aftermath of Hurricane Katrina, particularly in regard to construction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dorr, Kent A.; Ostrom, Michael J.; Freeman-Pollard, Jhivaun R.

CH2M Hill Plateau Remediation Company (CHPRC) designed, constructed, commissioned, and began operation of the largest groundwater pump and treatment facility in the U.S. Department of Energy's (DOE) nationwide complex. This one-of-a-kind groundwater pump and treatment facility, located at the Hanford Nuclear Reservation Site (Hanford Site) in Washington State, was built in an accelerated manner with American Recovery and Reinvestment Act (ARRA) funds and has attained Leadership in Energy and Environmental Design (LEED) GOLD certification, which makes it the first non-administrative building in the DOE Office of Environmental Management complex to earn such an award. There were many contractual, technical, configurationmore » management, quality, safety, and LEED challenges associated with the design, procurement, construction, and commissioning of this $95 million, 52,000 ft groundwater pump and treatment facility. This paper will present the Project and LEED accomplishments, as well as Lessons Learned by CHPRC when additional ARRA funds were used to accelerate design, procurement, construction, and commissioning of the 200 West Groundwater Pump and Treatment (2W P&T) Facility to meet DOE's mission of treating contaminated groundwater at the Hanford Site with a new facility by June 28, 2012.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dorr, Kent A.; Ostrom, Michael J.; Freeman-Pollard, Jhivaun R.

CH2M Hill Plateau Remediation Company (CHPRC) designed, constructed, commissioned, and began operation of the largest groundwater pump and treatment facility in the U.S. Department of Energy’s (DOE) nationwide complex. This one-of-a-kind groundwater pump and treatment facility, located at the Hanford Nuclear Reservation Site (Hanford Site) in Washington State, was built to an accelerated schedule with American Recovery and Reinvestment Act (ARRA) funds. There were many contractual, technical, configuration management, quality, safety, and Leadership in Energy and Environmental Design (LEED) challenges associated with the design, procurement, construction, and commissioning of this $95 million, 52,000 ft groundwater pump and treatment facility tomore » meet DOE’s mission objective of treating contaminated groundwater at the Hanford Site with a new facility by June 28, 2012. The project team’s successful integration of the project’s core values and green energy technology throughout design, procurement, construction, and start-up of this complex, first-of-its-kind Bio Process facility resulted in successful achievement of DOE’s mission objective, as well as attainment of LEED GOLD certification, which makes this Bio Process facility the first non-administrative building in the DOE Office of Environmental Management complex to earn such an award.« less

A divide-and-conquer algorithm for large-scale de novo transcriptome assembly through combining small assemblies from existing algorithms.

PubMed

Sze, Sing-Hoi; Parrott, Jonathan J; Tarone, Aaron M

2017-12-06

While the continued development of high-throughput sequencing has facilitated studies of entire transcriptomes in non-model organisms, the incorporation of an increasing amount of RNA-Seq libraries has made de novo transcriptome assembly difficult. Although algorithms that can assemble a large amount of RNA-Seq data are available, they are generally very memory-intensive and can only be used to construct small assemblies. We develop a divide-and-conquer strategy that allows these algorithms to be utilized, by subdividing a large RNA-Seq data set into small libraries. Each individual library is assembled independently by an existing algorithm, and a merging algorithm is developed to combine these assemblies by picking a subset of high quality transcripts to form a large transcriptome. When compared to existing algorithms that return a single assembly directly, this strategy achieves comparable or increased accuracy as memory-efficient algorithms that can be used to process a large amount of RNA-Seq data, and comparable or decreased accuracy as memory-intensive algorithms that can only be used to construct small assemblies. Our divide-and-conquer strategy allows memory-intensive de novo transcriptome assembly algorithms to be utilized to construct large assemblies.

Space construction system analysis. Part 2: Space construction experiments concepts

NASA Technical Reports Server (NTRS)

Boddy, J. A.; Wiley, L. F.; Gimlich, G. W.; Greenberg, H. S.; Hart, R. J.; Lefever, A. E.; Lillenas, A. N.; Totah, R. S.

1980-01-01

Technology areas in the orbital assembly of large space structures are addressed. The areas included structures, remotely operated assembly techniques, and control and stabilization. Various large space structure design concepts are reviewed and their construction procedures and requirements are identified.

State Requirements for Educational Facilities, 1997.

ERIC Educational Resources Information Center

Florida State Dept. of Education, Tallahassee. Office of Educational Facilities.

This document updates Florida's deregulation of construction of educational facilities guidelines, while keeping as the primary focus the safety of the students in pre-K through community college facilities. Organized by the sequence of steps required in the facilities procurement process, it covers general definitions, property…

40 CFR 247.10 - Paper and paper products.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Paper and paper products. 247.10... COMPREHENSIVE PROCUREMENT GUIDELINE FOR PRODUCTS CONTAINING RECOVERED MATERIALS Item Designations § 247.10 Paper and paper products. Paper and paper products, excluding building and construction paper grades. ...

40 CFR 247.10 - Paper and paper products.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Paper and paper products. 247.10... COMPREHENSIVE PROCUREMENT GUIDELINE FOR PRODUCTS CONTAINING RECOVERED MATERIALS Item Designations § 247.10 Paper and paper products. Paper and paper products, excluding building and construction paper grades. ...

Association between Organ Procurement Organization Social Network Centrality and Kidney Discard and Transplant Outcomes1

PubMed Central

Butala, Neel M.; King, Marissa D.; Reitsma, William; Formica, Richard N.; Abt, Peter L.; Reese, Peter P.; Parikh, Chirag R.

2015-01-01

Background Given growth in kidney transplant waitlists and discard rates, donor kidney acceptance is an important problem. We used network analysis to examine whether organ procurement organization (OPO) network centrality affects discard and outcomes. Methods We identified 106,160 deceased-donor kidneys recovered for transplant from 2000–2010 in SRTR. We constructed the transplant network by year with each OPO representing a node and each kidney-sharing relationship between OPOs representing a directed tie between nodes. Primary exposures were the number of different OPOs to which an OPO has given a kidney or from which an OPO has received a kidney in year preceding procurement year. Primary outcomes were discard, cold-ischemia time, delayed graft function, and 1-year graft loss. We used multivariable regression, restricting analysis to the 50% of OPOs with highest discard and stratifying remaining OPOs by kidney volume. Models controlled for kidney donor risk index, waitlist time, and kidney pumping. Results An increase in one additional OPO to which a kidney was given by a procuring OPO in a year was associated with 1.4% lower likelihood of discard for a given kidney (odds ratio, 0.986; 95% confidence interval, 0.974-0.998) among OPOs procuring high kidney volume, but 2% higher likelihood of discard (OR:1.021, CI:1.006, 1.037) among OPOs procuring low kidney volume, with mixed associations with recipient outcomes. Conclusions Our study highlights the value of network analysis in revealing how broader kidney sharing is associated with levels of organ acceptance. We conclude interventions to promote broader inter-OPO sharing could be developed to reduce discard for a subset of OPOs. PMID:26102610

Reiterative Recombination for the in vivo assembly of libraries of multigene pathways.

PubMed

Wingler, Laura M; Cornish, Virginia W

2011-09-13

The increasing sophistication of synthetic biology is creating a demand for robust, broadly accessible methodology for constructing multigene pathways inside of the cell. Due to the difficulty of rationally designing pathways that function as desired in vivo, there is a further need to assemble libraries of pathways in parallel, in order to facilitate the combinatorial optimization of performance. While some in vitro DNA assembly methods can theoretically make libraries of pathways, these techniques are resource intensive and inherently require additional techniques to move the DNA back into cells. All previously reported in vivo assembly techniques have been low yielding, generating only tens to hundreds of constructs at a time. Here, we develop "Reiterative Recombination," a robust method for building multigene pathways directly in the yeast chromosome. Due to its use of endonuclease-induced homologous recombination in conjunction with recyclable markers, Reiterative Recombination provides a highly efficient, technically simple strategy for sequentially assembling an indefinite number of DNA constructs at a defined locus. In this work, we describe the design and construction of the first Reiterative Recombination system in Saccharomyces cerevisiae, and we show that it can be used to assemble multigene constructs. We further demonstrate that Reiterative Recombination can construct large mock libraries of at least 10(4) biosynthetic pathways. We anticipate that our system's simplicity and high efficiency will make it a broadly accessible technology for pathway construction and render it a valuable tool for optimizing pathways in vivo.

Reiterative Recombination for the in vivo assembly of libraries of multigene pathways

PubMed Central

Wingler, Laura M.; Cornish, Virginia W.

2011-01-01

The increasing sophistication of synthetic biology is creating a demand for robust, broadly accessible methodology for constructing multigene pathways inside of the cell. Due to the difficulty of rationally designing pathways that function as desired in vivo, there is a further need to assemble libraries of pathways in parallel, in order to facilitate the combinatorial optimization of performance. While some in vitro DNA assembly methods can theoretically make libraries of pathways, these techniques are resource intensive and inherently require additional techniques to move the DNA back into cells. All previously reported in vivo assembly techniques have been low yielding, generating only tens to hundreds of constructs at a time. Here, we develop “Reiterative Recombination,” a robust method for building multigene pathways directly in the yeast chromosome. Due to its use of endonuclease-induced homologous recombination in conjunction with recyclable markers, Reiterative Recombination provides a highly efficient, technically simple strategy for sequentially assembling an indefinite number of DNA constructs at a defined locus. In this work, we describe the design and construction of the first Reiterative Recombination system in Saccharomyces cerevisiae, and we show that it can be used to assemble multigene constructs. We further demonstrate that Reiterative Recombination can construct large mock libraries of at least 104 biosynthetic pathways. We anticipate that our system’s simplicity and high efficiency will make it a broadly accessible technology for pathway construction and render it a valuable tool for optimizing pathways in vivo. PMID:21876185

The effects of intermittent hydrostatic pressure on self-assembled articular cartilage constructs.

PubMed

Hu, Jerry C; Athanasiou, Kyriacos A

2006-05-01

To date, static culture for the tissue engineering of articular cartilage has shown to be inadequate in conferring functionality to constructs. Various forms of mechanical stimuli accompany articular cartilage development in vivo, and one of these is hydrostatic pressure. This study used histology, biochemistry, and biomechanics to examine the effects of intermittent hydrostatic pressure, applied at 10 MPa and 1 Hz for 4 h per day for 5 days per week for up to 8 weeks on self-assembled chondrocyte constructs. The self-assembling process is a novel approach that allows engineering of articular cartilage constructs without the use of exogenous scaffolds. The self-assembled constructs were found to be capable of enduring this loading regimen. Significant increases in collagen production were only observed in pressurized samples. Intermittent hydrostatic pressure prevented a significant decrease in total GAG, which was significant in controls. Aside from the beneficial effects intermittent hydrostatic pressure may have on ECM synthesis, its effects on mechanical properties may require longer culture periods to manifest. This study demonstrates the successful use of the self-assembling process to produce articular cartilage constructs. It also shows for the first time that long-term culture of tissue-engineered articular cartilage construct benefits from intermittent hydrostatic pressure.

Research on construction quality and improvement of assembly construction

NASA Astrophysics Data System (ADS)

Cheng, Fei

2017-11-01

Based on the acceleration of the urbanization process and the improvement of the quality of life of our residents, the demand for building construction has been increasing. In this context, the construction industry in order to promote the construction efficiency, quality improvement, to meet the needs of the development of the times to strengthen the new technology, the use of new technologies. At present, China’s engineering construction units in the process of carrying out the project to strengthen the use of assembly-type construction technology, which thus achieved for the traditional construction work low-level, high time-consuming issues, and promote the steady improvement of production efficiency. Based on this, this paper focuses on the analysis of the connotation of the assembly structure and analyzes the quality problems in the construction process of the construction projects and puts forward the improvement measures to promote the improvement of the building quality and the construction of the building Construction speed. Based on this, this paper analyzes the structural system and design of prefabricated building.

Multicriteria Analysis of Assembling Buildings from Steel Frame Structures

NASA Astrophysics Data System (ADS)

Miniotaite, Ruta

2017-10-01

Steel frame structures are often used in the construction of public and industrial buildings. They are used for: all types of slope roofs; walls of newly-built public and industrial buildings; load bearing structures; roofs of renovated buildings. The process of assembling buildings from steel frame structures should be analysed as an integrated process influenced by such factors as construction materials and machinery used, the qualification level of construction workers, complexity of work, available finance. It is necessary to find a rational technological design solution for assembling buildings from steel frame structures by conducting a multiple criteria analysis. The analysis provides a possibility to evaluate the engineering considerations and find unequivocal solutions. The rational alternative of a complex process of assembling buildings from steel frame structures was found through multiple criteria analysis and multiple criteria evaluation. In multiple criteria evaluation of technological solutions for assembling buildings from steel frame structures by pairwise comparison method the criteria by significance are distributed as follows: durability is the most important criterion in the evaluation of alternatives; the price (EUR/unit of measurement) of a part of assembly process; construction workers’ qualification level (category); mechanization level of a part of assembling process (%), and complexity of assembling work (in points) are less important criteria.

Marketing human organs: the autonomy paradox.

PubMed

Marshall, P A; Thomasma, D C; Daar, A S

1996-03-01

The severe shortage of organs for transplantation and the continual reluctance of the public to voluntarily donate has prompted consideration of alternative strategies for organ procurement. This paper explores the development of market approaches for procuring human organs for transplantation and considers the social and moral implications of organ donation as both a "gift of life" and a "commodity exchange." The problematic and paradoxical articulation of individual autonomy in relation to property rights and marketing human body parts is addressed. We argue that beliefs about proprietorship over human body parts and the capacity to provide consent for organ donation are culturally constructed. We contend that the political and economic framework of biomedicine, in western and non-western nations, influences access to transplantation technology and shapes the form and development of specific market approaches. Finally, we suggest that marketing approaches for organ procurement are and will be negotiated within cultural parameters constrained by several factors: beliefs about the physical body and personhood, religious traditions, economic conditions, and the availability of technological resources.

Assembly planning based on subassembly extraction

NASA Technical Reports Server (NTRS)

Lee, Sukhan; Shin, Yeong Gil

1990-01-01

A method is presented for the automatic determination of assembly partial orders from a liaison graph representation of an assembly through the extraction of preferred subassemblies. In particular, the authors show how to select a set of tentative subassemblies by decomposing a liaison graph into a set of subgraphs based on feasibility and difficulty of disassembly, how to evaluate each of the tentative subassemblies in terms of assembly cost using the subassembly selection indices, and how to construct a hierarchical partial order graph (HPOG) as an assembly plan. The method provides an approach to assembly planning by identifying spatial parallelism in assembly as a means of constructing temporal relationships among assembly operations and solves the problem of finding a cost-effective assembly plan in a flexible environment. A case study of the assembly planning of a mechanical assembly is presented.

43 CFR 34.8 - Affirmative action plans.

Code of Federal Regulations, 2010 CFR

2010-10-01

... the employment of minorities and women and the utilization of MBE's and FBE's in the construction and... women, by each job group. (3) Goals should be set in proportion to the group's general availability in... dissemination of information on business opportunities and procurement practices to minority and women's...

7 CFR 1717.608 - RUS approval of contracts.

Code of Federal Regulations, 2014 CFR

2014-01-01

... engineering services, and in 7 CFR part 1726 for construction and procurement. (b) Large retail power contracts. RUS approval of contracts to sell electric power to retail customers is required only if the... AGRICULTURE POST-LOAN POLICIES AND PROCEDURES COMMON TO INSURED AND GUARANTEED ELECTRIC LOANS Operational...

7 CFR 1717.608 - RUS approval of contracts.

Code of Federal Regulations, 2013 CFR

2013-01-01

... engineering services, and in 7 CFR part 1726 for construction and procurement. (b) Large retail power contracts. RUS approval of contracts to sell electric power to retail customers is required only if the... AGRICULTURE POST-LOAN POLICIES AND PROCEDURES COMMON TO INSURED AND GUARANTEED ELECTRIC LOANS Operational...

7 CFR 1717.608 - RUS approval of contracts.

Code of Federal Regulations, 2012 CFR

2012-01-01

... engineering services, and in 7 CFR part 1726 for construction and procurement. (b) Large retail power contracts. RUS approval of contracts to sell electric power to retail customers is required only if the... AGRICULTURE POST-LOAN POLICIES AND PROCEDURES COMMON TO INSURED AND GUARANTEED ELECTRIC LOANS Operational...

7 CFR 1726.175 - General plant materials.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 11 2011-01-01 2011-01-01 false General plant materials. 1726.175 Section 1726.175... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES General Plant § 1726.175 General plant... determine the procurement method that best meets its needs for purchase of general plant material and...

7 CFR 1726.175 - General plant materials.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false General plant materials. 1726.175 Section 1726.175... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES General Plant § 1726.175 General plant... determine the procurement method that best meets its needs for purchase of general plant material and...

7 CFR 1726.175 - General plant materials.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 11 2012-01-01 2012-01-01 false General plant materials. 1726.175 Section 1726.175... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES General Plant § 1726.175 General plant... determine the procurement method that best meets its needs for purchase of general plant material and...

7 CFR 1726.175 - General plant materials.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 11 2013-01-01 2013-01-01 false General plant materials. 1726.175 Section 1726.175... AGRICULTURE ELECTRIC SYSTEM CONSTRUCTION POLICIES AND PROCEDURES General Plant § 1726.175 General plant... determine the procurement method that best meets its needs for purchase of general plant material and...

76 FR 29728 - Notice of Intent To Prepare an Environmental Impact Statement and Notice of Potential Floodplain...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-23

... 2.0 program would proceed through 2020 with design, construction, operation, and monitoring... of this test includes project definition, design, procurement, manufacture, installation, startup... the repowered oxy-combustion design. The station contains existing infrastructure that could support...

Department of the Army Justification of Estimates for Fiscal Year 1985. Procurement Appropriations-Construction Program Submitted to Congress February 1984. DD Forms 1391.

DTIC Science & Technology

1984-02-01

REQUIREMENT FOR CONSTRUCTION PROJECT: These buildings house electronic equipment, irreplaceable rocket motor design and performance records, a...consists of five subprojects which are to provide modern facilities designed for rocket motor production. These production efficient buildings will...replace facilities which were designed and built in 1930-1942 for artillery shell loading. Four of the sub- projects are for buildings while the fifth

Columbia Quilt

NASA Image and Video Library

2018-02-22

A certificate and quilt square are on display that confirms the transfer of a giant hand-made quilt in honor of space shuttle Columbia and her crew from the Office of Procurement to the Columbia Preservation Room inside the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida. The quilt was made by Katherine Walsh, a lifelong NASA and space program fan originally from Kentucky. The quilt will be displayed in the preservation room with its certificate as part of NASA's Apollo, Challenger, Columbia Lessons Learned Program.

The United States’ Vulnerability to Coercion by China in the Rare Earths Market

DTIC Science & Technology

2012-12-14

a computer, is assembled in a third country. This has brought down the total cost of such products which has had very positive effects on the...world’s economy. When materials or resources can be obtained from multiple sources around the world, costs of procurement and manufacture are lower, which...results in the total cost of goods being overall less expensive. Sometimes there is the possibility of a very dangerous side effect from relying 2

Lessons Learned from the 200 West Pump and Treatment Facility Construction Project at the US DOE Hanford Site - A Leadership for Energy and Environmental Design (LEED) Gold-Certified Facility - 13113

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dorr, Kent A.; Freeman-Pollard, Jhivaun R.; Ostrom, Michael J.

CH2M Hill Plateau Remediation Company (CHPRC) designed, constructed, commissioned, and began operation of the largest groundwater pump and treatment facility in the U.S. Department of Energy's (DOE) nationwide complex. This one-of-a-kind groundwater pump and treatment facility, located at the Hanford Nuclear Reservation Site (Hanford Site) in Washington State, was built to an accelerated schedule with American Recovery and Reinvestment Act (ARRA) funds. There were many contractual, technical, configuration management, quality, safety, and Leadership in Energy and Environmental Design (LEED) challenges associated with the design, procurement, construction, and commissioning of this $95 million, 52,000 ft groundwater pump and treatment facility tomore » meet DOE's mission objective of treating contaminated groundwater at the Hanford Site with a new facility by June 28, 2012. The project team's successful integration of the project's core values and green energy technology throughout design, procurement, construction, and start-up of this complex, first-of-its-kind Bio Process facility resulted in successful achievement of DOE's mission objective, as well as attainment of LEED GOLD certification (Figure 1), which makes this Bio Process facility the first non-administrative building in the DOE Office of Environmental Management complex to earn such an award. (authors)« less

FRIB cryogenic system status

NASA Astrophysics Data System (ADS)

Casagrande, F.; Ganni, V.; Knudsen, P.; Jones, S.; Sidi-Yekhlef, A.; Tatsumoto, H.; Nguyen, C.; Fila, A.; Vargas, G.; Dudley, C.; Joseph, N.; Stanley, S.; Dixon, K.; Norton, R.; Laverdure, N.; Yang, S.

2017-12-01

Construction and installation of the FRIB 4.5 K helium refrigeration system is nearing completion, with compressor system commissioning and 4.5 K refrigerator commissioning on schedule to occur in late 2017. The LINAC 4.5 K helium distribution system, all major process equipment, and the cryogenic distribution for the sub-systems have been procured and delivered. The sub-atmospheric cold box fabrication is planned to begin the summer of 2017, which is on schedule for commissioning in the spring of 2018. Commissioning of the support systems, such as the helium gas storage, helium purifier, and oil processor is planned to be complete by the summer of 2017. This paper presents details of the equipment procured, installation status and commissioning plans.

On-chip self-assembly of cell embedded microstructures to vascular-like microtubes.

PubMed

Yue, Tao; Nakajima, Masahiro; Takeuchi, Masaru; Hu, Chengzhi; Huang, Qiang; Fukuda, Toshio

2014-03-21

Currently, research on the construction of vascular-like tubular structures is a hot area of tissue engineering, since it has potential applications in the building of artificial blood vessels. In this paper, we report a fluidic self-assembly method using cell embedded microstructures to construct vascular-like microtubes. A novel 4-layer microfluidic device was fabricated using polydimethylsiloxane (PDMS), which contains fabrication, self-assembly and extraction areas inside one channel. Cell embedded microstructures were directly fabricated using poly(ethylene glycol) diacrylate (PEGDA) in the fabrication area, namely on-chip fabrication. Self-assembly of the fabricated microstructures was performed in the assembly area which has a micro well. Assembled tubular structures (microtubes) were extracted outside the channel into culture dishes using a normally closed (NC) micro valve in the extraction area. The self-assembly mechanism was experimentally demonstrated. The performance of the NC micro valve and embedded cell concentration were both evaluated. Fibroblast (NIH/3T3) embedded vascular-like microtubes were constructed inside this reusable microfluidic device.

An overview of the EASE/ACCESS space construction demonstration

NASA Technical Reports Server (NTRS)

Levin, George M.; Ross, Jerry L.; Spring, Sherwood C.

1988-01-01

Consideration is given to the development of the Experimental Assembly of Structures in EVA/Assembly Concept for Construction of Erectable Space Structures (EASE/ACCESS) space construction demonstration, which was performed during Space Shuttle mission 61-B. The mission equipment is described and illustrated and the EASE/ACCESS mission management structure is outlined. Simulations of the assembly and disassembly in the NASA neutral buoyancy simulators were used to test the mission plans. In addition, EVA training and crew performance for the mission are discussed.

Bipolar battery construction

NASA Technical Reports Server (NTRS)

Edwards, Dean B. (Inventor); Rippel, Wally E. (Inventor)

1981-01-01

A lightweight, bipolar battery construction for lead acid batteries in which a plurality of thin, rigid, biplates each comprise a graphite fiber thermoplastic composition in conductive relation to lead stripes plated on opposite flat surfaces of the plates, and wherein a plurality of nonconductive thermoplastic separator plates support resilient yieldable porous glass mats in which active material is carried, the biplates and separator plates with active material being contained and maintained in stacked assembly by axial compression of the stacked assembly. A method of assembling such a bipolar battery construction.

The optimal hormonal replacement modality selection for multiple organ procurement from brain-dead organ donors

PubMed Central

Mi, Zhibao; Novitzky, Dimitri; Collins, Joseph F; Cooper, David KC

2015-01-01

The management of brain-dead organ donors is complex. The use of inotropic agents and replacement of depleted hormones (hormonal replacement therapy) is crucial for successful multiple organ procurement, yet the optimal hormonal replacement has not been identified, and the statistical adjustment to determine the best selection is not trivial. Traditional pair-wise comparisons between every pair of treatments, and multiple comparisons to all (MCA), are statistically conservative. Hsu’s multiple comparisons with the best (MCB) – adapted from the Dunnett’s multiple comparisons with control (MCC) – has been used for selecting the best treatment based on continuous variables. We selected the best hormonal replacement modality for successful multiple organ procurement using a two-step approach. First, we estimated the predicted margins by constructing generalized linear models (GLM) or generalized linear mixed models (GLMM), and then we applied the multiple comparison methods to identify the best hormonal replacement modality given that the testing of hormonal replacement modalities is independent. Based on 10-year data from the United Network for Organ Sharing (UNOS), among 16 hormonal replacement modalities, and using the 95% simultaneous confidence intervals, we found that the combination of thyroid hormone, a corticosteroid, antidiuretic hormone, and insulin was the best modality for multiple organ procurement for transplantation. PMID:25565890

7 CFR 1753.48 - Procurement procedures.

Code of Federal Regulations, 2010 CFR

2010-01-01

...' qualifications for all types of construction and for the required information on the bidder and subcontractors... qualified to submit bids, RUS written approval must be obtained to proceed with requesting bids. (4) Bid...) Materials on hand to be furnished by the borrower shall be released to the contractor at the start of...

10 CFR 52.99 - Inspection during construction; ITAAC schedules and notifications; NRC notices.

Code of Federal Regulations, 2014 CFR

2014-01-01

...; ITAAC schedules and notifications; NRC notices. (a) Licensee schedule for completing inspections, tests... schedule for completing the inspections, tests, or analyses in the ITAAC. The licensee shall submit updates... combined license may proceed at its own risk with design and procurement activities, and a licensee may...

10 CFR 52.99 - Inspection during construction; ITAAC schedules and notifications; NRC notices.

Code of Federal Regulations, 2013 CFR

2013-01-01

...; ITAAC schedules and notifications; NRC notices. (a) Licensee schedule for completing inspections, tests... schedule for completing the inspections, tests, or analyses in the ITAAC. The licensee shall submit updates... combined license may proceed at its own risk with design and procurement activities, and a licensee may...

40 CFR 80.1352 - What are the pre-compliance reporting requirements for the gasoline benzene program?

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Benzene concentration. An estimate of the average gasoline benzene concentration corresponding to the time... engineering and permitting, Procurement and Construction, and Commissioning and startup. (7) Basic information regarding the selected technology pathway for compliance (e.g., precursor re-routing or other technologies...

23 CFR 140.908 - Materials and supplies.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Reimbursement for Railroad Work § 140.908 Materials and supplies. (a) Procurement. Materials and supplies, if... job, less a consideration for loss in service life at 10 percent for rails, angle bars, tie plates and... measures for restoration of affected areas as required by the physical construction or by reason of safety...

24 CFR 570.611 - Conflict of interest.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Conflict of interest. 570.611... § 570.611 Conflict of interest. (a) Applicability. (1) In the procurement of supplies, equipment, construction, and services by recipients and by subrecipients, the conflict of interest provisions in 24 CFR 85...

24 CFR 1003.606 - Conflict of interest.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Conflict of interest. 1003.606... Requirements § 1003.606 Conflict of interest. (a) Applicability. (1) In the procurement of supplies, equipment, construction, and services by grantees and subgrantees, the conflict of interest provisions in 24 CFR 85.36 and...

Selling to NASA

NASA Technical Reports Server (NTRS)

1981-01-01

The prospective NASA contractor is provided with information that describes the agency and its procurement practices. Products include ideas, manufacturing capabilities, fabricated components, construction, basic materials, and specialized services. NASA assistance in marketing these and other products is emphasized. Small and minority business enterprises are discussed. The agency's scientific and technical information activities are also discussed.

75 FR 59102 - Defense Federal Acquisition Regulation Supplement; Government-Assigned Serial Number Marking...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... requires agreement between the Government and contractor prior to use of the serial numbers in constructing... Parts 211 and 252 Government procurement. Ynette R. Shelkin, Editor, Defense Acquisition Regulations... revising the introductory text to read as follows: 252.211-7007 Reporting of Government-Furnished Equipment...

48 CFR 536.271 - Project labor agreements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... significant project means a Federal construction project with a total cost to the Federal Government of more... framework for labor-management cooperation to advance the Government's procurement interest in cost, efficiency, and quality. (d) Policy. (1) You may, on a project-by-project basis, use a PLA on a large and...

48 CFR 752.7009 - Marking.

Code of Federal Regulations, 2014 CFR

2014-10-01

... sites and other project locations be suitably marked with the USAID emblem. Shipping containers are also..., marking is not required for raw materials shipped in bulk (such as coal, grain, etc.), or for semifinished... procurement of commodities to be shipped, and as early as possible for project construction sites and other...

48 CFR 752.7009 - Marking.

Code of Federal Regulations, 2010 CFR

2010-10-01

... sites and other project locations be suitably marked with the USAID emblem. Shipping containers are also..., marking is not required for raw materials shipped in bulk (such as coal, grain, etc.), or for semifinished... procurement of commodities to be shipped, and as early as possible for project construction sites and other...

48 CFR 752.7009 - Marking.

Code of Federal Regulations, 2012 CFR

2012-10-01

... sites and other project locations be suitably marked with the USAID emblem. Shipping containers are also..., marking is not required for raw materials shipped in bulk (such as coal, grain, etc.), or for semifinished... procurement of commodities to be shipped, and as early as possible for project construction sites and other...

48 CFR 752.7009 - Marking.

Code of Federal Regulations, 2011 CFR

2011-10-01

... sites and other project locations be suitably marked with the USAID emblem. Shipping containers are also..., marking is not required for raw materials shipped in bulk (such as coal, grain, etc.), or for semifinished... procurement of commodities to be shipped, and as early as possible for project construction sites and other...

48 CFR 752.7009 - Marking.

Code of Federal Regulations, 2013 CFR

2013-10-01

... sites and other project locations be suitably marked with the USAID emblem. Shipping containers are also..., marking is not required for raw materials shipped in bulk (such as coal, grain, etc.), or for semifinished... procurement of commodities to be shipped, and as early as possible for project construction sites and other...

48 CFR 552.236-83 - Requirement for a Project Labor Agreement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... interest and concern, including productivity, quality of work, safety, and health. (4) The PLA shall fully... cooperation to advance the Government's procurement interest in cost, efficiency, and quality. (b) The...]. The PLA binds the Contractor and subcontractors of whatever tier engaged in onsite construction work...

New Technical Solution for Vertical Shaft Equipping Using Steel Headframe of Multifunction Purpose

NASA Astrophysics Data System (ADS)

Kassikhina, Elena; Pershin, Vladimir; Glazkov, Yurij

2017-11-01

The article reviews a novel approach to the design of steel angle headframe for vertical shafts of coal and ore mines on the basis of rational design solutions. Practice of construction of coal and ore mines provides application of various designs for steel angle headframes which are divided into separate large assembly blocks and constructive elements during assembling operations. Design of these blocks and elements, their weight and dimensions effect the chose of the method of assembling on which economic and technological indicators, as well as duration of down-time, depend on during performance of construction operations in shaft. The technical solution on equipment provision for mine vertical shaft using headframe of multifunctional purpose will allow changing the management construction of vertical shaft. The constructive design of the headgear allows application of the effective method of assembly and thus to provide improvement of the technical and economic indexes, and high calendar time rate of the shaft construction due to reduction of duration of works on equipment provision for the shaft and to refurbishment of the shaft in order to carry out horizontal mining.

Marginal Bidding: An Application of the Equimarginal Principle to Bidding in TAC SCM

NASA Astrophysics Data System (ADS)

Greenwald, Amy; Naroditskiy, Victor; Odean, Tyler; Ramirez, Mauricio; Sodomka, Eric; Zimmerman, Joe; Cutler, Clark

We present a fast and effective bidding strategy for the Trading Agent Competition in Supply Chain Management (TAC SCM). In TAC SCM, manufacturers compete to procure computer parts from suppliers (the procurement problem), and then sell assembled computers to customers in reverse auctions (the bidding problem). This paper is concerned only with bidding, in which an agent must decide how many computers to sell and at what prices to sell them. We propose a greedy solution, Marginal Bidding, inspired by the Equimarginal Principle, which states that revenue is maximized among possible uses of a resource when the return on the last unit of the resource is the same across all areas of use. We show experimentally that certain variations of Marginal Bidding can compute bids faster than our ILP solution, which enables Marginal Bidders to consider future demand as well as current demand, and hence achieve greater revenues when knowledge of the future is valuable.

Real-Time Probing of Nanowire Assembly Kinetics at the Air-Water Interface by In Situ Synchrotron X-Ray Scattering.

PubMed

He, Zhen; Jiang, Hui-Jun; Wu, Long-Long; Liu, Jian-Wei; Wang, Geng; Wang, Xiao; Wang, Jin-Long; Hou, Zhong-Huai; Chen, Gang; Yu, Shu-Hong

2018-07-02

Although many assembly strategies have been used to successfully construct well-aligned nanowire (NW) assemblies, the understanding of their assembly kinetics has remained elusive, which restricts the development of NW-based device and circuit fabrication. Now a versatile strategy that combines interfacial assembly and synchrotron-based grazing-incidence small-angle X-ray scattering (GISAXS) is presented to track the assembly evolution of the NWs in real time. During the interface assembly process, the randomly dispersed NWs gradually aggregate to form small ordered NW-blocks and finally are constructed into well-defined NW monolayer driven by the conformation entropy. The NW assembly mechanism can be well revealed by the thermodynamic analysis and large-scale molecular dynamics theoretical evaluation. These findings point to new opportunities for understanding NW assembly kinetics and manipulating NW assembled structures by bottom-up strategy. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Special Inspector General for Iraq Reconstruction: Quarterly Report and Semiannual Report to the United States Congress

DTIC Science & Technology

2007-07-30

enacted November 14, 2005 • P.L. 109-148, Department of Defense, Emergency Supplemental Appropria- tions To Address Hurricanes in the Gulf of Mexico ...CEFMS IRRF NON- CONSTRUCTION THROUGH JUNE 2007 4256 W91GET-07-C-0301-NA-0001 FALCON SECURITY LTD *URI#27076* PROVIDE PERSONNEL ADN EQUIPMENT WITH... TUBERCULOSIS SCREENING EQUIPMENT FOR CTRS NC-EQUIPMENT PROCUREMENT AND MODERNIZATION NON-CONSTRUCTION $1,363,058.43 $1,363,058.43 $0.00 CEFMS IRRF NON

Special Inspector General for Iraq Reconstruction. Quarterly and Semiannual Report to the United States Congress

DTIC Science & Technology

2007-07-30

of Mexico , and Pandemic Influenza Act, 2006, enacted December 30, 2005 • P.L. 109-234, The Emergency Supplemental Appropriations Act for Defense...1,561,750.00 $0.00 CEFMS IRRF NON- CONSTRUCTION THROUGH JUNE 2007 4256 W91GET-07-C-0301-NA-0001 FALCON SECURITY LTD *URI#27076* PROVIDE PERSONNEL ADN EQUIPMENT... TUBERCULOSIS SCREENING EQUIPMENT FOR CTRS NC-EQUIPMENT PROCUREMENT AND MODERNIZATION NON-CONSTRUCTION $1,363,058.43 $1,363,058.43 $0.00 CEFMS IRRF

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2010-08-02

and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f...has been required, disrupting the optimal construction sequence and application of lessons learned for follow-on vessels in these programs... NGSS ) ability to recover in the aftermath of Hurricane Katrina, particularly in regard to construction of LPD 17 Class vessels.” • “I am equally

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2010-07-07

and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f...has been required, disrupting the optimal construction sequence and application of lessons learned for follow-on vessels in these programs... NGSS ) ability to recover in the aftermath of Hurricane Katrina, particularly in regard to construction of LPD 17 Class vessels.” • “I am equally

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2010-10-04

Issues, and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f...rework has been required, disrupting the optimal construction sequence and application of lessons learned for follow-on vessels in these programs...Systems’ ( NGSS ) ability to recover in the aftermath of Hurricane Katrina, particularly in regard to construction of LPD 17 Class vessels.” • “I am

DNA Assembly Line for Nano-Construction

ScienceCinema

Oleg Gang

2017-12-09

Building on the idea of using DNA to link up nanoparticles scientists at Brookhaven National Lab have designed a molecular assembly line for high-precision nano-construction. Nanofabrication is essential for exploiting the unique properties of nanoparticl

Assembling a Space Station in orbit

NASA Technical Reports Server (NTRS)

Brand, Vance D.; Lounge, J. Michael; Walker, David M.

1990-01-01

The factors affecting the degree of difficulty of assembling a Space Station in orbit and ways of arriving at the optimum construction solution are briefly reviewed and applied to the Space Station Freedom (SSF). The assembly of the SSF navigation and control systems and the relevant tools and methods are examined along with the characteristics of early assembly flights. The most significant challenges facing the construction of the SSF are discussed, and new technologies which will be incorporated into the SSF are briefly considered.

Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).

PubMed

Chandran, Sunil

2017-01-01

The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the ligase chain reaction (LCR). The LCR method utilizes non-overlapping DNA parts that are ligated together with the guidance of bridging oligos. Using this method, we have successfully assembled up to 20 DNA parts in a single reaction or DNA constructs up to 26 kb in size.

GENESUS: a two-step sequence design program for DNA nanostructure self-assembly.

PubMed

Tsutsumi, Takanobu; Asakawa, Takeshi; Kanegami, Akemi; Okada, Takao; Tahira, Tomoko; Hayashi, Kenshi

2014-01-01

DNA has been recognized as an ideal material for bottom-up construction of nanometer scale structures by self-assembly. The generation of sequences optimized for unique self-assembly (GENESUS) program reported here is a straightforward method for generating sets of strand sequences optimized for self-assembly of arbitrarily designed DNA nanostructures by a generate-candidates-and-choose-the-best strategy. A scalable procedure to prepare single-stranded DNA having arbitrary sequences is also presented. Strands for the assembly of various structures were designed and successfully constructed, validating both the program and the procedure.

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

PubMed Central

Binder, Andreas; Lambert, Jayne; Morbitzer, Robert; Popp, Claudia; Ott, Thomas; Lahaye, Thomas; Parniske, Martin

2014-01-01

The Golden Gate (GG) modular assembly approach offers a standardized, inexpensive and reliable way to ligate multiple DNA fragments in a pre-defined order in a single-tube reaction. We developed a GG based toolkit for the flexible construction of binary plasmids for transgene expression in plants. Starting from a common set of modules, such as promoters, protein tags and transcribed regions of interest, synthetic genes are assembled, which can be further combined to multigene constructs. As an example, we created T-DNA constructs encoding multiple fluorescent proteins targeted to distinct cellular compartments (nucleus, cytosol, plastids) and demonstrated simultaneous expression of all genes in Nicotiana benthamiana, Lotus japonicus and Arabidopsis thaliana. We assembled an RNA interference (RNAi) module for the construction of intron-spliced hairpin RNA constructs and demonstrated silencing of GFP in N. benthamiana. By combination of the silencing construct together with a codon adapted rescue construct into one vector, our system facilitates genetic complementation and thus confirmation of the causative gene responsible for a given RNAi phenotype. As proof of principle, we silenced a destabilized GFP gene (dGFP) and restored GFP fluorescence by expression of a recoded version of dGFP, which was not targeted by the silencing construct. PMID:24551083

Programmable DNA tile self-assembly using a hierarchical sub-tile strategy.

PubMed

Shi, Xiaolong; Lu, Wei; Wang, Zhiyu; Pan, Linqiang; Cui, Guangzhao; Xu, Jin; LaBean, Thomas H

2014-02-21

DNA tile based self-assembly provides a bottom-up approach to construct desired nanostructures. DNA tiles have been directly constructed from ssDNA and readily self-assembled into 2D lattices and 3D superstructures. However, for more complex lattice designs including algorithmic assemblies requiring larger tile sets, a more modular approach could prove useful. This paper reports a new DNA 'sub-tile' strategy to easily create whole families of programmable tiles. Here, we demonstrate the stability and flexibility of our sub-tile structures by constructing 3-, 4- and 6-arm DNA tiles that are subsequently assembled into 2D lattices and 3D nanotubes according to a hierarchical design. Assembly of sub-tiles, tiles, and superstructures was analyzed using polyacrylamide gel electrophoresis and atomic force microscopy. DNA tile self-assembly methods provide a bottom-up approach to create desired nanostructures; the sub-tile strategy adds a useful new layer to this technique. Complex units can be made from simple parts. The sub-tile approach enables the rapid redesign and prototyping of complex DNA tile sets and tiles with asymmetric designs.

Mesoscale Graphene-like Honeycomb Mono- and Multilayers Constructed via Self-Assembly of Coclusters.

PubMed

Hou, Xue-Sen; Zhu, Guo-Long; Ren, Li-Jun; Huang, Zi-Han; Zhang, Rui-Bin; Ungar, Goran; Yan, Li-Tang; Wang, Wei

2018-02-07

Honeycomb structure endows graphene with extraordinary properties. But could a honeycomb monolayer superlattice also be generated via self-assembly of colloids or nanoparticles? Here we report the construction of mono- and multilayer molecular films with honeycomb structure that can be regarded as self-assembled artificial graphene (SAAG). We construct fan-shaped molecular building blocks by covalently connecting two kinds of clusters, one polyoxometalate and four polyhedral oligomeric silsesquioxanes. The precise shape control enables these complex molecules to self-assemble into a monolayer 2D honeycomb superlattice that mirrors that of graphene but on the mesoscale. The self-assembly of the SAAG was also reproduced via coarse-grained molecular simulations of a fan-shaped building block. It revealed a hierarchical process and the key role of intermediate states in determining the honeycomb structure. Experimental images also show a diversity of bi- and trilayer stacking modes. The successful creation of SAAG and its stacks opens up prospects for the preparation of novel self-assembled nanomaterials with unique properties.

Sharing University Intellectual Capital: The Role of Continuing Education

ERIC Educational Resources Information Center

O'Neill, Hugh

2005-01-01

New England's research universities are widely recognized as major contributors to the region's economy. In many of the communities in which they are located, they rank among the largest employers. They purchase goods and services from New England companies, and are often among the leading procurers of construction activity. Each year, they bring…

EPA Form 5700‑52A: United States Environmental Protection Agency Minority Business Enterprise/Woman Business Enterprise Utilization under Federal Grants and Cooperative Agreements

EPA Pesticide Factsheets

MBE/WBE utilization is based on 40 CFR Part 33. EPA Form 5700-52A must be completed by recipients of Federal grants, cooperative agreements, or other Federal financial assistance which involve procurement of supplies, equipment, construction, etc.

49 CFR 238.103 - Fire safety.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Fire safety. 238.103 Section 238.103... § 238.103 Fire safety. (a) Materials. (1) Materials used in constructing a passenger car or a cab of a... section at the time it was tested. (c) Fire safety analysis for procuring new passenger cars and...

49 CFR 238.103 - Fire safety.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Fire safety. 238.103 Section 238.103... § 238.103 Fire safety. (a) Materials. (1) Materials used in constructing a passenger car or a cab of a... section at the time it was tested. (c) Fire safety analysis for procuring new passenger cars and...

49 CFR 238.103 - Fire safety.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Fire safety. 238.103 Section 238.103... § 238.103 Fire safety. (a) Materials. (1) Materials used in constructing a passenger car or a cab of a... section at the time it was tested. (c) Fire safety analysis for procuring new passenger cars and...

7 CFR 1753.48 - Procurement procedures.

Code of Federal Regulations, 2011 CFR

2011-01-01

... that is estimated to cost $250,000, or less, inclusive of labor and materials. (2) The procedures to be...) Materials on hand, until released to the contractor, shall be covered by fire and either wind-storm or... construction are necessary, and the cost of such changes or corrections is properly chargeable to the borrower...

7 CFR 1753.48 - Procurement procedures.

Code of Federal Regulations, 2012 CFR

2012-01-01

... that is estimated to cost $250,000, or less, inclusive of labor and materials. (2) The procedures to be...) Materials on hand, until released to the contractor, shall be covered by fire and either wind-storm or... construction are necessary, and the cost of such changes or corrections is properly chargeable to the borrower...

7 CFR 1753.48 - Procurement procedures.

Code of Federal Regulations, 2013 CFR

2013-01-01

... that is estimated to cost $250,000, or less, inclusive of labor and materials. (2) The procedures to be...) Materials on hand, until released to the contractor, shall be covered by fire and either wind-storm or... construction are necessary, and the cost of such changes or corrections is properly chargeable to the borrower...

7 CFR 1753.48 - Procurement procedures.

Code of Federal Regulations, 2014 CFR

2014-01-01

... that is estimated to cost $250,000, or less, inclusive of labor and materials. (2) The procedures to be...) Materials on hand, until released to the contractor, shall be covered by fire and either wind-storm or... construction are necessary, and the cost of such changes or corrections is properly chargeable to the borrower...

45 CFR 1357.32 - State fiscal requirements (title IV-B, subpart 2, family preservation and family support services).

Code of Federal Regulations, 2010 CFR

2010-10-01

... following: Procurement; payroll; personnel functions; management, maintenance and operation of space and... SERVICES THE ADMINISTRATION ON CHILDREN, YOUTH AND FAMILIES, FOSTER CARE MAINTENANCE PAYMENTS, ADOPTION... title IV-B may not be used for the purchase or construction of facilities. (f) Maintenance of effort...

48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... into the end product (whether or not such costs are paid to a domestic firm), and any applicable duty.... Designated country means— (1) A World Trade Organization Government Procurement Agreement (WTO GPA) country..., Singapore, Slovak Republic, Slovenia, Spain, Sweden, Switzerland, Taiwan (known in the World Trade...

48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... into the end product (whether or not such costs are paid to a domestic firm), and any applicable duty.... Designated country means— (1) A World Trade Organization Government Procurement Agreement (WTO GPA) country..., Singapore, Slovak Republic, Slovenia, Spain, Sweden, Switzerland, Taiwan (known in the World Trade...

48 CFR 252.225-7045 - Balance of Payments Program-Construction Material Under Trade Agreements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... into the end product (whether or not such costs are paid to a domestic firm), and any applicable duty.... Designated country means— (1) A World Trade Organization Government Procurement Agreement (WTO GPA) country..., Romania, Singapore, Slovak Republic, Slovenia, Spain, Sweden, Switzerland, Taiwan (known in the World...

Brassboard Astrometric Beam Combiner (ABC) Development for the Space Interferometry Mission (SIM)

NASA Technical Reports Server (NTRS)

Jeganathan, Muthu; Kuan, Gary; Rud, Mike; Lin, Sean; Sutherland, Kristen; Moore, James; An, Xin

2008-01-01

The Astrometric Beam Combiner (ABC) is a critical element of the Space Interferometry Mission (SIM) that performs three key functions: coherently combine starlight from two siderostats; individually detect starlight for angle tracking; and disperse and detect the interferometric fringes. In addition, the ABC contains: a stimulus, cornercubes and shutters for in-orbit calibration; several tip/tilt mirror mechanisms for in-orbit alignment; and internal metrology beam launcher for pathlength monitoring. The detailed design of the brassboard ABC (which has the form, fit and function of the flight unit) is complete, procurement of long-lead items is underway, and assembly and testing is expected to be completed in Spring 2009. In this paper, we present the key requirements for the ABC, details of the completed optical and mechanical design as well as plans for assembly and alignment.

Processing and thermodynamics research, Volume II. Monthly progress report, November 1984

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Data assembly is in progress on the Venezuelan Cerro Negro, California, Wilmington and Mexican Mayan crudes. A draft of a proposal is in preparation that might join this correlational work with complementary correlational work at the University of Oklahoma (Project BPT1). Cerro Negro 200 to 425/sup 0/C and 425 to 550/sup 0/C saturate fractions were analyzed with the CEC 21-103 mass spectrometer using ASTM method D2786, and experiments were devised to establish reproducibility of analyses with the KRATOS MS-50 (Project BPT2). Thermodynamic property measurements on organic nitrogen compounds included a liquid phase heat capacity study of 2,5-dimethylpyridine, and the oxygenmore » sensitivity of phenanthridine was checked prior to bomb calorimetry (Project BPT3A). Heat capacity measurements on chroman are progressing well, and vapor pressure measurements of tetrahydrophenanthrene are in progress (Project BPT3B). Work was begun on the design and procurement of equipment necessary for construction of a bench scale catalytic cracking unit. The supercritical extraction unit is being assembled (Project OPT1). Thiopene separations were begun on Cerro Negro 425 to 550/sup 0/C neutrals, and separation of sulfides from Cerro Negro 550 to 700/sup 0/C and 700+/sup 0/C neutrals was completed (Project OPT2). Preparations for PVT studies of methanol are nearing completion, and a list of critically needed PVT measurements was reviewed to help select the next compound for study. A possible candidate is hydrogen sulfide. (Project OPT3). Good progress continues in the treatment of the RP feed (Project OPT4) to recover Milspec F-76. It is anticipated that 85% recovery is possible by the chemical treatment/distillation process.« less

DNA-templated photonic arrays and assemblies: design principles and future opportunities.

PubMed

Su, Wu; Bonnard, Vanessa; Burley, Glenn A

2011-07-11

Molecular photonics is a rapidly developing and multi-disciplinary field of research involving the construction of molecular assemblies comprising photoactive building blocks that are responsive to a light stimulus. A salient challenge in this field is the controlled assembly of these building blocks with nanoscale precision. DNA exhibits considerable promise as an architecture for the templated assembly of photoactive materials. In this Concept Article we describe the progress that has been made in the area of DNA photonics, in which DNA acts as a platform for the construction of optoelectronic assemblies, thin films and devices. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Construction and assembly of the wire planes for the MicroBooNE Time Projection Chamber

DOE Office of Scientific and Technical Information (OSTI.GOV)

Acciarri, R.; Adams, C.; Asaadi, J.

In this paper we describe how the readout planes for the MicroBooNE Time Projection Chamber were constructed, assembled and installed. We present the individual wire preparation using semi-automatic winding machines and the assembly of wire carrier boards. The details of the wire installation on the detector frame and the tensioning of the wires are given. A strict quality assurance plan ensured the integrity of the readout planes. The different tests performed at all stages of construction and installation provided crucial information to achieve the successful realization of the MicroBooNE wire planes.

Definition of large components assembled on-orbit and robot compatible mechanical joints

NASA Technical Reports Server (NTRS)

Williamsen, J.; Thomas, F.; Finckenor, J.; Spiegel, B.

1990-01-01

One of four major areas of project Pathfinder is in-space assembly and construction. The task of in-space assembly and construction is to develop the requirements and the technology needed to build elements in space. A 120-ft diameter tetrahedral aerobrake truss is identified as the focus element. A heavily loaded mechanical joint is designed to robotically assemble the defined aerobrake element. Also, typical large components such as habitation modules, storage tanks, etc., are defined, and attachment concepts of these components to the tetrahedral truss are developed.

Space Habitat, assembly and repair facility

NASA Technical Reports Server (NTRS)

Colangelo, Todd A.; Hoetger, Debora C.; Kuo, Addison C.; Lo, Michael C.; Marcus, Leland R.; Tran, Phillip P.; Tutt, Chris J.; Wassmuth, Chad M.; Wildgrube, Gregory M.

1992-01-01

Integrated Space Systems (ISS) has designed a Low Earth Orbit Assembly Facility for submission in the 1992 AIAA/LORAL Team Space Design Competition. This facility, the Space Habitat, Assembly, and Repair Center (SHARC), will be used to construct, assemble, and service space vehicles. SHARC's primary mission will be the construction of interplanetary vehicles, but it will also be able to perform repair and refueling operations of craft which are in an Earth orbit. This facility has been designed using only present and near-present technology. The emphasis is on minimizing cost.

SIRTF primary mirror design, analysis, and testing

NASA Technical Reports Server (NTRS)

Sarver, George L., III; Maa, Scott; Chang, LI

1990-01-01

The primary mirror assembly (PMA) requirements and concepts for the Space Infrared Telescope Facility (SIRTF) program are discussed. The PMA studies at NASA/ARC resulted in the design of two engineering test articles, the development of a mirror mount cryogenic static load testing system, and the procurement and partial testing of a full scale spherical mirror mounting system. Preliminary analysis and testing of the single arch mirror with conical mount design and the structured mirror with the spherical mount design indicate that the designs will meet all figure and environmental requirements of the SIRTF program.

Interleaved array antenna technology development

NASA Technical Reports Server (NTRS)

1985-01-01

This is the third phase of a program to establish an antenna concept for shuttle and free flying spacecraft earth resources experiments using Synthetic Aperture Radar. The feasibility of a plated graphite epoxy waveguide for a space antenna was evaluated. A quantity of flat panels and waveguides were developed, procured, and tested for electrical and mechanical properties. In addition, processes for the assembly of a unique waveguide array were investigated. Finally, trades between various configurations that would allow elevation (range) electronic scanning and that would minimize feed complexity for various RF bandwidths were made.

Rational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single Reaction.

PubMed

Birla, Bhagyashree S; Chou, Hui-Hsien

2015-01-01

Gene synthesis is frequently used in modern molecular biology research either to create novel genes or to obtain natural genes when the synthesis approach is more flexible and reliable than cloning. DNA chemical synthesis has limits on both its length and yield, thus full-length genes have to be hierarchically constructed from synthesized DNA fragments. Gibson Assembly and its derivatives are the simplest methods to assemble multiple double-stranded DNA fragments. Currently, up to 12 dsDNA fragments can be assembled at once with Gibson Assembly according to its vendor. In practice, the number of dsDNA fragments that can be assembled in a single reaction are much lower. We have developed a rational design method for gene construction that allows high-number dsDNA fragments to be assembled into full-length genes in a single reaction. Using this new design method and a modified version of the Gibson Assembly protocol, we have assembled 3 different genes from up to 45 dsDNA fragments at once. Our design method uses the thermodynamic analysis software Picky that identifies all unique junctions in a gene where consecutive DNA fragments are specifically made to connect to each other. Our novel method is generally applicable to most gene sequences, and can improve both the efficiency and cost of gene assembly.

☆DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus.

PubMed

Zou, Xiao-Hui; Bi, Zhi-Xiang; Guo, Xiao-Juan; Zhang, Zun; Zhao, Yang; Wang, Min; Zhu, Ya-Lu; Jie, Hong-Ying; Yu, Yang; Hung, Tao; Lu, Zhuo-Zhuang

2018-07-01

Plasmid bearing adenovirus genome is generally constructed with the method of homologous recombination in E. coli BJ5183 strain. Here, we utilized Gibson gene assembly technique to generate infectious clone of fowl adenovirus 4 (FAdV-4). Primers flanked with partial inverted terminal repeat (ITR) sequence of FAdV-4 were synthesized to amplify a plasmid backbone containing kanamycin-resistant gene and pBR322 origin (KAN-ORI). DNA assembly was carried out by combining the KAN-ORI fragment, virus genomic DNA and DNA assembly master mix. E. coli competent cells were transformed with the assembled product, and plasmids (pKFAV4) were extracted and confirmed to contain viral genome by restriction analysis and sequencing. Virus was successfully rescued from linear pKFAV4-transfected chicken LMH cells. This approach was further verified in cloning of human adenovirus 5 genome. Our results indicated that DNA assembly technique simplified the construction of infectious clone of adenovirus, suggesting its possible application in virus traditional or reverse genetics. Copyright © 2018 Elsevier B.V. All rights reserved.

Recuperator assembly and procedures

DOEpatents

Kang, Yungmo; McKeirnan, Jr., Robert D.

2008-08-26

A construction of recuperator core segments is provided which insures proper assembly of the components of the recuperator core segment, and of a plurality of recuperator core segments. Each recuperator core segment must be constructed so as to prevent nesting of fin folds of the adjacent heat exchanger foils of the recuperator core segment. A plurality of recuperator core segments must be assembled together so as to prevent nesting of adjacent fin folds of adjacent recuperator core segments.

Visit from JAXA to NASA MSFC: The Engines Element & Ideas for Collaboration

NASA Technical Reports Server (NTRS)

Greene, William D.

2013-01-01

System Design, Development, and Fabrication: Design, develop, and fabricate or procure MB-60 component hardware compliant with the imposed technical requirements and in sufficient quantities to fulfill the overall MB-60 development effort. System Development, Assembly, and Test: Manage the scope of the development, assembly, and test-related activities for MB-60 development. This scope includes engine-level development planning, engine assembly and disassembly, test planning, engine testing, inspection, anomaly resolution, and development of necessary ground support equipment and special test equipment. System Integration: Provide coordinated integration in the realms of engineering, safety, quality, and manufacturing disciplines across the scope of the MB-60 design and associated products development Safety and Mission Assurance, structural design, fracture control, materials and processes, thermal analysis. Systems Engineering and Analysis: Manage and perform Systems Engineering and Analysis to provide rigor and structure to the overall design and development effort for the MB-60. Milestone reviews, requirements management, system analysis, program management support Program Management: Manage, plan, and coordinate the activities across all portions of the MB-60 work scope by providing direction for program administration, business management, and supplier management.

A biological approach to assembling tissue modules through endothelial capillary network formation.

PubMed

Riesberg, Jeremiah J; Shen, Wei

2015-09-01

To create functional tissues having complex structures, bottom-up approaches to assembling small tissue modules into larger constructs have been emerging. Most of these approaches are based on chemical reactions or physical interactions at the interface between tissue modules. Here we report a biological assembly approach to integrate small tissue modules through endothelial capillary network formation. When adjacent tissue modules contain appropriate extracellular matrix materials and cell types that support robust endothelial capillary network formation, capillary tubules form and grow across the interface, resulting in assembly of the modules into a single, larger construct. It was shown that capillary networks formed in modules of dense fibrin gels seeded with human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs); adjacent modules were firmly assembled into an integrated construct having a strain to failure of 117 ± 26%, a tensile strength of 2208 ± 83 Pa and a Young's modulus of 2548 ± 574 Pa. Under the same culture conditions, capillary networks were absent in modules of dense fibrin gels seeded with either HUVECs or MSCs alone; adjacent modules disconnected even when handled gently. This biological assembly approach eliminates the need for chemical reactions or physical interactions and their associated limitations. In addition, the integrated constructs are prevascularized, and therefore this bottom-up assembly approach may also help address the issue of vascularization, another key challenge in tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.

Self-assembly of coiled coil peptides into nanoparticles vs 2-d plates: effects of assembly pathway

NASA Astrophysics Data System (ADS)

Kim, Kyunghee; Pochan, Darrin

Molecular solution assembly, or self-assembly, is a process by which ordered nanostructures or patterns are formed by non-covalent interactions during assembly. Biomimicry, the use of bioinspired molecules or biologically relevant materials, is an important area of self-assembly research with peptides serving a critical role as molecular tools. The morphology of peptide assemblies can be controlled by adjusting solution conditions such as the concentration of peptides, the temperature, and pH. Herein, spherical nanostructures, which have potential for creating an encapsulation system, are formed by self-assembly when coiled coil peptides are combined in solution. These peptides are homotrimeric and heterodimeric coiled-coil bundles and the homotrimer is connected with each of heterodimer through their external surfaces via disulfide bonds. The resultant covalent constructs could co-assemble into complementary trimeric hubs, respectively. The two peptide constructs are directly mixed and assembled in solution in order to produce either spherical particles or 2-d plates depending on the solution conditions and kinetic pathway of assembly. In particular, structural changes of the self-assembled peptides are explored by control of the thermal history of the assembly solution.

Cooling assembly for fuel cells

DOEpatents

Kaufman, Arthur; Werth, John

1990-01-01

A cooling assembly for fuel cells having a simplified construction whereby coolant is efficiently circulated through a conduit arranged in serpentine fashion in a channel within a member of such assembly. The channel is adapted to cradle a flexible, chemically inert, conformable conduit capable of manipulation into a variety of cooling patterns without crimping or otherwise restricting of coolant flow. The conduit, when assembled with the member, conforms into intimate contact with the member for good thermal conductivity. The conduit is non-corrodible and can be constructed as a single, manifold-free, continuous coolant passage means having only one inlet and one outlet.

Self-assembly and transformation of hybrid nano-objects and nanostructures under equilibrium and non-equilibrium conditions

NASA Astrophysics Data System (ADS)

Mann, Stephen

2009-10-01

Understanding how chemically derived processes control the construction and organization of matter across extended and multiple length scales is of growing interest in many areas of materials research. Here we review present equilibrium and non-equilibrium self-assembly approaches to the synthetic construction of discrete hybrid (inorganic-organic) nano-objects and higher-level nanostructured networks. We examine a range of synthetic modalities under equilibrium conditions that give rise to integrative self-assembly (supramolecular wrapping, nanoscale incarceration and nanostructure templating) or higher-order self-assembly (programmed/directed aggregation). We contrast these strategies with processes of transformative self-assembly that use self-organizing media, reaction-diffusion systems and coupled mesophases to produce higher-level hybrid structures under non-equilibrium conditions. Key elements of the constructional codes associated with these processes are identified with regard to existing theoretical knowledge, and presented as a heuristic guideline for the rational design of hybrid nano-objects and nanomaterials.

Marshall Space Flight Center's role in EASE/ACCESS mission management

NASA Technical Reports Server (NTRS)

Hawkins, Gerald W.

1987-01-01

The Marshall Space Flight Center (MSFC) Spacelab Payload Project Office was responsible for the mission management and development of several successful payloads. Two recent space construction experiments, the Experimental Assembly of Structures in Extravehicular Activity (EASE) and the Assembly Concept for Construction of Erectable Space Structures (ACCESS), were combined into a payload managed by the center. The Ease/ACCESS was flown aboard the Space Shuttle Mission 61-B. The EASE/ACCESS experiments were the first structures assembled in space, and the method used to manage this successful effort will be useful for future space construction missions. The MSFC mission management responsibilities for the EASE/ACCESS mission are addressed and how the lessons learned from the mission can be applied to future space construction projects are discussed.

Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy.

PubMed

Sarrion-Perdigones, Alejandro; Palaci, Jorge; Granell, Antonio; Orzaez, Diego

2014-01-01

GoldenBraid (GB) is an iterative and standardized DNA assembling system specially designed for Multigene Engineering in Plant Synthetic Biology. GB is based on restriction-ligation reactions using type IIS restriction enzymes. GB comprises a collection of standard DNA pieces named "GB parts" and a set of destination plasmids (pDGBs) that incorporate the multipartite assembly of standardized DNA parts. GB reactions are extremely efficient: two transcriptional units (TUs) can be assembled from several basic GBparts in one T-DNA less than 24 h. Moreover, larger assemblies comprising 4-5 TUs are routinely built in less than 2 working weeks. Here we provide a detailed view of the GB methodology. As a practical example, a Bimolecular Fluorescence Complementation construct comprising four TUs in a 12 kb DNA fragment is presented.

Photovoltaic system with improved AC connections and method of making same

DOEpatents

Cioffi, Philip Michael; Todorovic, Maja Harfman; Herzog, Michael Scott; Korman, Charles Steven; Doherty, Donald M.; Johnson, Neil Anthony

2018-02-13

An alternating current (AC) harness for a photovoltaic (PV) system includes a wire assembly having a first end and a second end, the wire assembly having a plurality of lead wires, and at least one AC connection module positioned at a location along a length of the wire assembly between the first end and the second end. Further, the at least one AC connection module includes a first connection terminal electrically coupled to the plurality of lead wires of the wire assembly and constructed to electrically couple the wire assembly with an output of a first PV module of the PV system. The at least one AC connection module also includes a second connection terminal electrically coupled to the plurality of lead wires of the wire assembly and constructed to electrically couple the wire assembly with an output of a second PV module of the PV system.

j5 DNA assembly design automation.

PubMed

Hillson, Nathan J

2014-01-01

Modern standardized methodologies, described in detail in the previous chapters of this book, have enabled the software-automated design of optimized DNA construction protocols. This chapter describes how to design (combinatorial) scar-less DNA assembly protocols using the web-based software j5. j5 assists biomedical and biotechnological researchers construct DNA by automating the design of optimized protocols for flanking homology sequence as well as type IIS endonuclease-mediated DNA assembly methodologies. Unlike any other software tool available today, j5 designs scar-less combinatorial DNA assembly protocols, performs a cost-benefit analysis to identify which portions of an assembly process would be less expensive to outsource to a DNA synthesis service provider, and designs hierarchical DNA assembly strategies to mitigate anticipated poor assembly junction sequence performance. Software integrated with j5 add significant value to the j5 design process through graphical user-interface enhancement and downstream liquid-handling robotic laboratory automation.

Implementation of Building Information Modeling (BIM) in Construction: A Comparative Case Study

NASA Astrophysics Data System (ADS)

Rowlinson, Steve; Collins, Ronan; Tuuli, Martin M.; Jia, Yunyan

2010-05-01

Building Information Modeling (BIM) approach is increasingly adopted in coordination of construction projects, with a number of parties providing BIM services and software solutions. However, the empirical impact of BIM on construction industry has yet to be investigated. This paper explores the interaction between BIM and the construction industry during its implementation, with a specific focus on the empirical impacts of BIM on the design and construction processes and professional roles during the process. Two cases were selected from recent construction projects coordinated with BIM systems: the Venetian Casino project in Macau and the Cathy Pacific Cargo Terminal project in Hong Kong. The former case illustrates how the conflicts emerged during the design process and procurement were addressed by adopting a BIM approach. The latter demonstrates how the adoption of BIM altered the roles of architect, contractor, and sub-contractors involved in the project. The impacts of BIM were critically reviewed and discussed.

Ionic self-assembly for functional hierarchical nanostructured materials.

PubMed

Faul, Charl F J

2014-12-16

CONSPECTUS: The challenge of constructing soft functional materials over multiple length scales can be addressed by a number of different routes based on the principles of self-assembly, with the judicious use of various noncovalent interactions providing the tools to control such self-assembly processes. It is within the context of this challenge that we have extensively explored the use of an important approach for materials construction over the past decade: exploiting electrostatic interactions in our ionic self-assembly (ISA) method. In this approach, cooperative assembly of carefully chosen charged surfactants and oppositely charged building blocks (or tectons) provides a facile noncovalent route for the rational design and production of functional nanostructured materials. Generally, our research efforts have developed with an initial focus on establishing rules for the construction of novel noncovalent liquid-crystalline (LC) materials. We found that the use of double-tailed surfactant species (especially branched double-tailed surfactants) led to the facile formation of thermotropic (and, in certain cases, lyotropic) phases, as demonstrated by extensive temperature-dependent X-ray and light microscopy investigations. From this core area of activity, research expanded to cover issues beyond simple construction of anisotropic materials, turning to the challenge of inclusion and exploitation of switchable functionality. The use of photoactive azobenzene-containing ISA materials afforded opportunities to exploit both photo-orientation and surface relief grating formation. The preparation of these anisotropic LC materials was of interest, as the aim was the facile production of disposable and low-cost optical components for display applications and data storage. However, the prohibitive cost of the photo-orientation processes hampered further exploitation of these materials. We also expanded our activities to explore ISA of biologically relevant tectons, specifically deoxyguanosine monophosphate. This approach proved, in combination with block copolymer (BCP) self-assembly, very fruitful for the construction of complex and hierarchical functional materials across multiple length scales. Molecular frustration and incommensurability, which played a major role in structure formation in combination with nucleotide assembly, have now become important tools to tune supramolecular structure formation. These concepts, that is, the use of BCP assembly and incommensurability, in combination with metal-containing polymeric materials, have provided access to novel supramolecular morphologies and, more importantly, design rules to prepare such constructs. These design rules are now also being applied to the assembly of electroactive oligo(aniline)-based materials for the preparation of highly ordered functional soft materials, and present an opportunity for materials development for applications in energy storage. In this Account, we therefore discuss investigations into (i) the inclusion and preparation of supramolecular photoactive and electroactive materials; (ii) the exploitation and control over multiple noncovalent interactions to fine-tune function, internal structure, and long-range order and (iii) exploration of construction over multiple length scales by combination of ISA with well-known BCP self-assembly. Combination of ISA with tuning of volume fractions, mutual compatibility, and molecular frustration now provides a versatile tool kit to construct complex and hierarchical functional materials in a facile noncovalent way. A direct challenge for future ISA activities would certainly be the construction of functional mesoscale objects. However, within a broader scientific context, the challenge would be to exploit this powerful assembly tool for application in areas of research with societal impact, for example, energy storage and generation. The hope is that this Account will provide a platform for such future research activities and opportunities.

49 CFR 393.45 - Brake tubing and hoses; hose assemblies and end fittings.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Brake tubing and hoses; hose assemblies and end... assemblies and end fittings. (a) General construction requirements for tubing and hoses, assemblies, and end fittings. All brake tubing and hoses, brake hose assemblies, and brake hose end fittings must meet the...

49 CFR 393.45 - Brake tubing and hoses; hose assemblies and end fittings.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Brake tubing and hoses; hose assemblies and end... assemblies and end fittings. (a) General construction requirements for tubing and hoses, assemblies, and end fittings. All brake tubing and hoses, brake hose assemblies, and brake hose end fittings must meet the...

49 CFR 393.45 - Brake tubing and hoses; hose assemblies and end fittings.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Brake tubing and hoses; hose assemblies and end... assemblies and end fittings. (a) General construction requirements for tubing and hoses, assemblies, and end fittings. All brake tubing and hoses, brake hose assemblies, and brake hose end fittings must meet the...

Space construction system analysis. Part 2: Construction analysis

NASA Technical Reports Server (NTRS)

Roebuck, J. A.; Buck, P. A.; Gimlich, G. W.; Greenberg, H. S.; Hart, R. J.; Indrikis, J.; Lefever, A. E.; Lillenas, A. N.; Mcbaine, C. K.

1980-01-01

The construction methods specific to the end to end construction process for building the ETVP in low Earth orbit, using the space shuttle orbiter as a construction base, are analyzed. The analyses concerned three missions required to build the basic platform. The first mission involved performing the fabrication of beams in space and assembling the beams into a basic structural framework. The second mission was to install the forward support structure and aft support structure, the forward assembly, and a TT&C antenna. The third mission plan was to complete the construction of the platform and activate it to begin operations in low Earth orbit. The integration of the activities for each mission is described along with the construction requirements and construction logic.

Single- and Multi-Prime Contracting in North Carolina Public Construction.

ERIC Educational Resources Information Center

Bluestein, Frayda S.

1995-01-01

The North Carolina General Assembly directed the State Building Commission to study the comparative costs of multi- and single-prime contracting and report the results to the 1995 General Assembly. Describes the analysis of data collected from governmental units that had awarded construction contracts. Identifies some alternative contracting…

Two Rotor Stratified Charge Rotary Engine (SCRE) Engine System Technology Evaluation

NASA Technical Reports Server (NTRS)

Hoffman, T.; Mack, J.; Mount, R.

1994-01-01

This report summarizes results of an evaluation of technology enablement component technologies as integrated into a two rotor Stratified Charge Rotary Engine (SCRE). The work constitutes a demonstration of two rotor engine system technology, utilizing upgraded and refined component technologies derived from prior NASA Contracts NAS3-25945, NAS3-24628 and NAS-23056. Technical objectives included definition of, procurement and assembly of an advanced two rotor core aircraft engine, operation with Jet-A fuel at Take-Off rating of 340 BHP (254kW) and operation at a maximum cruise condition of 255 BHP (190kW), 75% cruise. A fuel consumption objective of 0.435 LBS/BHP-Hr (265 GRS/kW-Hr) was identified for the maximum cruise condition. A critical technology component item, a high speed, unit injector fuel injection system with electronic control was defined, procured and tested in conjunction with this effort. The two rotor engine configuration established herein defines an affordable, advanced, Jet-A fuel capability core engine (not including reduction gear, propeller shaft and some aircraft accessories) for General Aviation of the mid-1990's and beyond.

YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae

PubMed Central

Guo, Yakun; Dong, Junkai; Zhou, Tong; Auxillos, Jamie; Li, Tianyi; Zhang, Weimin; Wang, Lihui; Shen, Yue; Luo, Yisha; Zheng, Yijing; Lin, Jiwei; Chen, Guo-Qiang; Wu, Qingyu; Cai, Yizhi; Dai, Junbiao

2015-01-01

It is a routine task in metabolic engineering to introduce multicomponent pathways into a heterologous host for production of metabolites. However, this process sometimes may take weeks to months due to the lack of standardized genetic tools. Here, we present a method for the design and construction of biological parts based on the native genes and regulatory elements in Saccharomyces cerevisiae. We have developed highly efficient protocols (termed YeastFab Assembly) to synthesize these genetic elements as standardized biological parts, which can be used to assemble transcriptional units in a single-tube reaction. In addition, standardized characterization assays are developed using reporter constructs to calibrate the function of promoters. Furthermore, the assembled transcription units can be either assayed individually or applied to construct multi-gene metabolic pathways, which targets a genomic locus or a receiving plasmid effectively, through a simple in vitro reaction. Finally, using β-carotene biosynthesis pathway as an example, we demonstrate that our method allows us not only to construct and test a metabolic pathway in several days, but also to optimize the production through combinatorial assembly of a pathway using hundreds of regulatory biological parts. PMID:25956650

Systems engineering studies of on-orbit assembly operation

NASA Technical Reports Server (NTRS)

Morgenthaler, George W.

1991-01-01

While the practice of construction has a long history, the underlying theory of construction is relatively young. Very little has been documented as to techniques of logistic support, construction planning, construction scheduling, construction testing, and inspection. The lack of 'systems approaches' to construction processes is certainly one of the most serious roadblocks to the construction of space structures. System engineering research efforts at CSC are aimed at developing concepts and tools which contribute to a systems theory of space construction. The research is also aimed at providing means for trade-offs of design parameters for other research areas in CSC. Systems engineering activity at CSC has divided space construction into the areas of orbital assembly, lunar base construction, interplanetary transport vehicle construction, and Mars base construction. A brief summary of recent results is given. Several models for 'launch-on-time' were developed. Launch-on-time is a critical concept to the assembly of such Earth-orbiting structures as the Space Station Freedom, and to planetary orbiters such as the Mars transfer vehicle. CSC has developed a launch vehicle selection model which uses linear programming to find optimal combinations of launch vehicles of various sizes (Atlas, Titan, Shuttles, HLLV's) to support SEI missions. Recently, the Center developed a cost trade-off model for studying on orbit assembly logistics. With this model it was determined that the most effective size of the HLLV would be in the range of 120 to 200 metric tons to LEO, which is consistent with the choices of General Stafford's Synthesis Group Report. A second-generation Dynamic Construction Activities Model ('DYCAM') process model has been under development, based on our past results in interruptability and our initial DYCAM model. This second-generation model is built on the paradigm of knowledge-based expert systems. It is aimed at providing answers to two questions: (1) what are some necessary or sufficient conditions for judging conceptual designs of spacecraft?, and (2) can a methodology be formulated such that these conditions may be used to provide computer-aided tools for evaluating conceptual designs and planning for space assembly sequences? Early simulation results indicate that the DYCAM model has a clear ability to emulate and simulate human orbital construction processes.

Emerging Technologies for Assembly of Microscale Hydrogels

PubMed Central

Kavaz, Doga; Demirel, Melik C.; Demirci, Utkan

2013-01-01

Assembly of cell encapsulating building blocks (i.e., microscale hydrogels) has significant applications in areas including regenerative medicine, tissue engineering, and cell-based in vitro assays for pharmaceutical research and drug discovery. Inspired by the repeating functional units observed in native tissues and biological systems (e.g., the lobule in liver, the nephron in kidney), assembly technologies aim to generate complex tissue structures by organizing microscale building blocks. Novel assembly technologies enable fabrication of engineered tissue constructs with controlled properties including tunable microarchitectural and predefined compositional features. Recent advances in micro- and nano-scale technologies have enabled engineering of microgel based three dimensional (3D) constructs. There is a need for high-throughput and scalable methods to assemble microscale units with a complex 3D micro-architecture. Emerging assembly methods include novel technologies based on microfluidics, acoustic and magnetic fields, nanotextured surfaces, and surface tension. In this review, we survey emerging microscale hydrogel assembly methods offering rapid, scalable microgel assembly in 3D, and provide future perspectives and discuss potential applications. PMID:23184717

Varactor diode assembly with low parasitic reactances

NASA Technical Reports Server (NTRS)

Dickens, L. E.

1975-01-01

Development of varactor diode assembly overcomes parasitic reactances of conventional varactor packages. In specially constructed assembly very high idler-frequency to signal-frequency ratios are used to obtain low-noise operation over maximum bandwidth.

Decorating multi-walled carbon nanotubes with quantum dots for construction of multi-color fluorescent nanoprobes.

PubMed

Jia, Nengqin; Lian, Qiong; Tian, Zhong; Duan, Xin; Yin, Min; Jing, Lihong; Chen, Shouhui; Shen, Hebai; Gao, Mingyuan

2010-01-29

Novel multi-color fluorescent nanoprobes were prepared by electrostatically assembling differently sized CdTe quantum dots on polyethylenimine (PEI) functionalized multi-walled carbon nanotubes (MWNTs). The structural and optical properties of the nano-assemblies (MWNTs-PEI-CdTe) were characterized by transmission electron microscopy (TEM), electron diffraction spectra (EDS), Raman spectroscopy, confocal microscopy and photoluminescence spectroscopy (PL), respectively. Electrochemical impedance spectroscopy (EIS) was also applied to investigate the electrostatic assembling among oxidized MWNTs, PEI and CdTe. Furthermore, confocal fluorescence microscopy was used to monitor the nano-assemblies' delivery into tumor cells. It was found that the nano-assemblies exhibit efficient intracellular transporting and strong intracellular tracking. These properties would make this luminescent nano-assembly an excellent building block for the construction of intracellular nanoprobes, which could hold great promise for biomedical applications.

Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system

PubMed Central

Kretschmer, Carola; Gruetzner, Ramona; Löfke, Christian; Dagdas, Yasin; Bürstenbinder, Katharina; Marillonnet, Sylvestre

2018-01-01

Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. Here, a toolkit containing further modules for the novel DNA assembly standards was developed. Intended for use with Modular Cloning, most modules are also compatible with GoldenBraid. Firstly, a collection of approximately 80 additional phytobricks is provided, comprising e.g. modules for inducible expression systems, promoters or epitope tags. Furthermore, DNA modules were developed for connecting Modular Cloning and Gateway cloning, either for toggling between systems or for standardized Gateway destination vector assembly. Finally, first instances of a “peripheral infrastructure” around Modular Cloning are presented: While available toolkits are designed for the assembly of plant transformation constructs, vectors were created to also use coding sequence-containing phytobricks directly in yeast two hybrid interaction or bacterial infection assays. The presented material will further enhance versatility of hierarchical DNA assembly strategies. PMID:29847550

An Analytic Model for DoD Divestments

DTIC Science & Technology

2015-04-30

Modernization (Research, Development, Test, & Evaluation [RDT&E], Procurement, Military Construction, Science , & Technology, and Weapons Acquisition), and...from the insurance industry as variables that impact actuarial calculations are also worth considering here. For example, as the insurer of national...initiatives (GAO-14-134). Washington, DC: Author. Hardin, G. (1968). The tragedy of the commons. Science , 162, 1243–1248. Harrison, T. (2012, August

76 FR 68027 - Federal Acquisition Regulation; Certification Requirement and Procurement Prohibition Relating to...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-02

.... Applicability to Construction Comment: One respondent was concerned that the prescription at FAR 25.1103, which.... 121(c); 10 U.S.C. chapter 137; and 42 U.S.C. 2473(c). 0 2. Amend section 25.1103 by revising paragraph (e) to read as follows: 25.1103 Other provisions and clauses. * * * * * (e) The contracting officer...

A new building block for DNA network formation by self-assembly and polymerase chain reaction.

PubMed

Bußkamp, Holger; Keller, Sascha; Robotta, Marta; Drescher, Malte; Marx, Andreas

2014-01-01

The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired by naturally existing self-assembled DNA architectures, branched DNA has been developed that allows self-assembly to predesigned architectures with dimensions on the nanometer scale. DNA is an attractive material for generation of nanostructures due to a plethora of enzymes which modify DNA with high accuracy, providing a toolbox for many different manipulations to construct nanometer scaled objects. We present a straightforward synthesis of a rigid DNA branching building block successfully used for the generation of DNA networks by self-assembly and network formation by enzymatic DNA synthesis. The Y-shaped 3-armed DNA construct, bearing 3 primer strands is accepted by Taq DNA polymerase. The enzyme uses each arm as primer strand and incorporates the branched construct into large assemblies during PCR. The networks were investigated by agarose gel electrophoresis, atomic force microscopy, dynamic light scattering, and electron paramagnetic resonance spectroscopy. The findings indicate that rather rigid DNA networks were formed. This presents a new bottom-up approach for DNA material formation and might find applications like in the generation of functional hydrogels.

Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria

PubMed Central

Taton, Arnaud; Unglaub, Federico; Wright, Nicole E.; Zeng, Wei Yue; Paz-Yepes, Javier; Brahamsha, Bianca; Palenik, Brian; Peterson, Todd C.; Haerizadeh, Farzad; Golden, Susan S.; Golden, James W.

2014-01-01

Inspired by the developments of synthetic biology and the need for improved genetic tools to exploit cyanobacteria for the production of renewable bioproducts, we developed a versatile platform for the construction of broad-host-range vector systems. This platform includes the following features: (i) an efficient assembly strategy in which modules released from 3 to 4 donor plasmids or produced by polymerase chain reaction are assembled by isothermal assembly guided by short GC-rich overlap sequences. (ii) A growing library of molecular devices categorized in three major groups: (a) replication and chromosomal integration; (b) antibiotic resistance; (c) functional modules. These modules can be assembled in different combinations to construct a variety of autonomously replicating plasmids and suicide plasmids for gene knockout and knockin. (iii) A web service, the CYANO-VECTOR assembly portal, which was built to organize the various modules, facilitate the in silico construction of plasmids, and encourage the use of this system. This work also resulted in the construction of an improved broad-host-range replicon derived from RSF1010, which replicates in several phylogenetically distinct strains including a new experimental model strain Synechocystis sp. WHSyn, and the characterization of nine antibiotic cassettes, four reporter genes, four promoters, and a ribozyme-based insulator in several diverse cyanobacterial strains. PMID:25074377

Construction of a standard test assembly for controlled laser studies in tissues: Preliminary study on human bone material

NASA Astrophysics Data System (ADS)

Beer, Franziska; Passow, Harald

2008-02-01

The aim of the study is the construction of a test assembly, which facilitates objective, comparative studies on the cutting performance of lasers in hard tissue. To ensure the applicability of our own construction for the reproducible performance of laser incisions in hard tissue, eleven freshly extracted blocks (2×1.5cm2) of human bone were prepared with a Er,Cr:YSGG laser by using a handheld handpiece, respectively, using the constructed device for a standardized cutting. A total of 44 cuts were executed. The specimen were then histologically evaluated. The standard test assembly met the requirements concerning the provision of objective results. The findings of the histological evaluation prove the reproducibility of the results. The standard test assembly presented in this paper facilitates comparative studies of different laser systems by reducing subjective influence on the preparation to a minimum. The results of this preliminary study show that the precision of the guiding instrument for laser cutting reduces the error of cut width by 50-fold, from 50to1μm.

Long-range energy transfer in self-assembled quantum dot-DNA cascades

NASA Astrophysics Data System (ADS)

Goodman, Samuel M.; Siu, Albert; Singh, Vivek; Nagpal, Prashant

2015-11-01

The size-dependent energy bandgaps of semiconductor nanocrystals or quantum dots (QDs) can be utilized in converting broadband incident radiation efficiently into electric current by cascade energy transfer (ET) between layers of different sized quantum dots, followed by charge dissociation and transport in the bottom layer. Self-assembling such cascade structures with angstrom-scale spatial precision is important for building realistic devices, and DNA-based QD self-assembly can provide an important alternative. Here we show long-range Dexter energy transfer in QD-DNA self-assembled single constructs and ensemble devices. Using photoluminescence, scanning tunneling spectroscopy, current-sensing AFM measurements in single QD-DNA cascade constructs, and temperature-dependent ensemble devices using TiO2 nanotubes, we show that Dexter energy transfer, likely mediated by the exciton-shelves formed in these QD-DNA self-assembled structures, can be used for efficient transport of energy across QD-DNA thin films.The size-dependent energy bandgaps of semiconductor nanocrystals or quantum dots (QDs) can be utilized in converting broadband incident radiation efficiently into electric current by cascade energy transfer (ET) between layers of different sized quantum dots, followed by charge dissociation and transport in the bottom layer. Self-assembling such cascade structures with angstrom-scale spatial precision is important for building realistic devices, and DNA-based QD self-assembly can provide an important alternative. Here we show long-range Dexter energy transfer in QD-DNA self-assembled single constructs and ensemble devices. Using photoluminescence, scanning tunneling spectroscopy, current-sensing AFM measurements in single QD-DNA cascade constructs, and temperature-dependent ensemble devices using TiO2 nanotubes, we show that Dexter energy transfer, likely mediated by the exciton-shelves formed in these QD-DNA self-assembled structures, can be used for efficient transport of energy across QD-DNA thin films. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr04778a

Self-Assembly: How Nature Builds

ERIC Educational Resources Information Center

Jones, M. Gail; Falvo, Michael R.; Broadwell, Bethany; Dotger, Sharon

2006-01-01

Self-assembly or spontaneous assembly is a process in which materials build themselves without assistance. This process plays a central role in the construction of biological structures and materials such as cells, viruses, and bone, and also in abiotic processes like phase transitions and crystal formation. The principles of self-assembly help…

Computer-aided design of nano-filter construction using DNA self-assembly

NASA Astrophysics Data System (ADS)

Mohammadzadegan, Reza; Mohabatkar, Hassan

2007-01-01

Computer-aided design plays a fundamental role in both top-down and bottom-up nano-system fabrication. This paper presents a bottom-up nano-filter patterning process based on DNA self-assembly. In this study we designed a new method to construct fully designed nano-filters with the pores between 5 nm and 9 nm in diameter. Our calculations illustrated that by constructing such a nano-filter we would be able to separate many molecules.

Application of multi-criteria decision analysis in selecting of sustainable investments

NASA Astrophysics Data System (ADS)

Kozik, Renata

2017-07-01

Investors of construction projects, especially financed with public money, quite slowly adapt environmentally friendly solutions, e.g. passive buildings. Practice shows that the use of green public procurement among the public investors is negligible. Energy-saving technologies and equipment are expensive at the construction phase and investors less or not at all take into account the future operating costs. The aim of this article is to apply the method of multi-criteria analysis ELECTRE to select the best investment in terms of cost of implementation, operation, as well as the impact on the environment.

79. Neg. No. F61A, Apr 13, 1930, INTERIORASSEMBLY BUILDING, BODY ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

79. Neg. No. F-61A, Apr 13, 1930, INTERIOR-ASSEMBLY BUILDING, BODY CONSTRUCTION - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

16. Exterior of assembly building (mold loft #2) used for ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

16. Exterior of assembly building (mold loft #2) used for runabout production, constructed CA. 1932. Most of the production run boats were assembled here. - Barbour Boat Works, Tryon Palace Drive, New Bern, Craven County, NC

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

PubMed Central

Shao, Zengyi; Zhao, Hua; Zhao, Huimin

2009-01-01

The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cerevisiae. We show that DNA assembler can rapidly assemble a functional d-xylose utilization pathway (∼9 kb DNA consisting of three genes), a functional zeaxanthin biosynthesis pathway (∼11 kb DNA consisting of five genes) and a functional combined d-xylose utilization and zeaxanthin biosynthesis pathway (∼19 kb consisting of eight genes) with high efficiencies (70–100%) either on a plasmid or on a yeast chromosome. As this new method only requires simple DNA preparation and one-step yeast transformation, it represents a powerful tool in the construction of biochemical pathways for synthetic biology, metabolic engineering and functional genomics studies. PMID:19074487

Microgripper construction kit

NASA Astrophysics Data System (ADS)

Gengenbach, Ulrich K.; Hofmann, Andreas; Engelhardt, Friedhelm; Scharnowell, Rudolf; Koehler, Bernd

2001-10-01

A large number of microgrippers has been developed in industry and academia. Although the importance of hybrid integration techniques and hence the demand for assembly tools grows continuously a large part of these developments has not yet been used in industrial production. The first grippers developed for microassembly were basically vacuum grippers and downscaled tweezers. Due to increasingly complex assembly tasks more and more functionality such as sensing or additional functions such as adhesive dispensing has been integrated into gripper systems over the last years. Most of these gripper systems are incompatible since there exists no standard interface to the assembly machine and no standard for the internal modules and interfaces. Thus these tools are not easily interchangeable between assembly machines and not easily adaptable to assembly tasks. In order to alleviate this situation a construction kit for modular microgrippers is being developed. It is composed of modules with well defined interfaces that can be combined to build task specific grippers. An abstract model of a microgripper is proposed as a tool to structure the development of the construction kit. The modular concept is illustrated with prototypes.

n/a

NASA Image and Video Library

1985-11-01

The crew assigned to the STS-61B mission included (kneeling left to right) Bryan D. O’conner, pilot; and Brewster H. Shaw, commander. On the back row, left to right, are Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission’s primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Autralia); and SATCOM KU-2 (RCA Americom. Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, VA and Marshall Space Flight Center (MSFC), the Assembly Concept for Construction of Erectable Space Structures (ACCESS) was developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). The primary objective of this experiment was to test the ACCESS structural assembly concept for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Construction and experimental validation of a simple, compact, resealable, and reliable Vycor{sup ®} superleak assembly for use at low temperatures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jahromi, Amir E.; Miller, Franklin K.

A new method of constructing a superleak assembly for use in experiments involving {sup 4}He or {sup 3}He-{sup 4}He mixtures at very low temperatures is described. Superleaks are made of a porous medium with very small pores and channels. Superleaks are often incorporated in thermomechanical pumps, superfluid magnetic pumps, dilution refrigerators, and superfluid helium transfer systems. We used several cylindrical pieces of Vycor, a permeable glass with average pore diameter of 40 Å and porosity of 28%, as a candidate to be used in our superleak assembly. Our design is simple and compact. Our superleak assembly can be disassembled andmore » easily reassembled for reuse. We successfully tested and validated this device at temperatures between 1.4 K and 2.7 K. We experienced no superfluid leaks into the surrounding vacuum. We also report that thermal cycling caused no performance degradation. It is our goal to share the design and construction techniques of this new superleak assembly.« less

STS-61B Crew Portrait

NASA Technical Reports Server (NTRS)

1985-01-01

The crew assigned to the STS-61B mission included (kneeling left to right) Bryan D. O'conner, pilot; and Brewster H. Shaw, commander. On the back row, left to right, are Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Autralia); and SATCOM KU-2 (RCA Americom. Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, VA and Marshall Space Flight Center (MSFC), the Assembly Concept for Construction of Erectable Space Structures (ACCESS) was developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). The primary objective of this experiment was to test the ACCESS structural assembly concept for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Electrodynamic tailoring of self-assembled three-dimensional electrospun constructs

NASA Astrophysics Data System (ADS)

Reis, Tiago C.; Correia, Ilídio J.; Aguiar-Ricardo, Ana

2013-07-01

The rational design of three-dimensional electrospun constructs (3DECs) can lead to striking topographies and tailored shapes of electrospun materials. This new generation of materials is suppressing some of the current limitations of the usual 2D non-woven electrospun fiber mats, such as small pore sizes or only flat shaped constructs. Herein, we pursued an explanation for the self-assembly of 3DECs based on electrodynamic simulations and experimental validation. We concluded that the self-assembly process is driven by the establishment of attractive electrostatic forces between the positively charged aerial fibers and the already collected ones, which tend to acquire a negatively charged network oriented towards the nozzle. The in situ polarization degree is strengthened by higher amounts of clustered fibers, and therefore the initial high density fibrous regions are the preliminary motifs for the self-assembly mechanism. As such regions increase their in situ polarization electrostatic repulsive forces will appear, favoring a competitive growth of these self-assembled fibrous clusters. Highly polarized regions will evidence higher distances between consecutive micro-assembled fibers (MAFs). Different processing parameters - deposition time, electric field intensity, concentration of polymer solution, environmental temperature and relative humidity - were evaluated in an attempt to control material's design.The rational design of three-dimensional electrospun constructs (3DECs) can lead to striking topographies and tailored shapes of electrospun materials. This new generation of materials is suppressing some of the current limitations of the usual 2D non-woven electrospun fiber mats, such as small pore sizes or only flat shaped constructs. Herein, we pursued an explanation for the self-assembly of 3DECs based on electrodynamic simulations and experimental validation. We concluded that the self-assembly process is driven by the establishment of attractive electrostatic forces between the positively charged aerial fibers and the already collected ones, which tend to acquire a negatively charged network oriented towards the nozzle. The in situ polarization degree is strengthened by higher amounts of clustered fibers, and therefore the initial high density fibrous regions are the preliminary motifs for the self-assembly mechanism. As such regions increase their in situ polarization electrostatic repulsive forces will appear, favoring a competitive growth of these self-assembled fibrous clusters. Highly polarized regions will evidence higher distances between consecutive micro-assembled fibers (MAFs). Different processing parameters - deposition time, electric field intensity, concentration of polymer solution, environmental temperature and relative humidity - were evaluated in an attempt to control material's design. Electronic supplementary information (ESI) available. See DOI: 10.1039/c3nr01668d

Mobile assemblies of Bennett linkages from four-crease origami patterns

NASA Astrophysics Data System (ADS)

Zhang, Xiao; Chen, Yan

2018-02-01

This paper deals with constructing mobile assemblies of Bennett linkages inspired by four-crease origami patterns. A transition technique has been proposed by taking the thick-panel form of an origami pattern as an intermediate bridge. A zero-thickness rigid origami pattern and its thick-panel form share the same sector angles and folding behaviours, while the thick-panel origami and the mobile assembly of linkages are kinematically equivalent with differences only in link profiles. Applying this transition technique to typical four-crease origami patterns, we have found that the Miura-ori and graded Miura-ori patterns lead to assemblies of Bennett linkages with identical link lengths. The supplementary-type origami patterns with different mountain-valley crease assignments correspond to different types of Bennett linkage assemblies with negative link lengths. And the identical linkage-type origami pattern generates a new mobile assembly. Hence, the transition technique offers a novel approach to constructing mobile assemblies of spatial linkages from origami patterns.

Mobile assemblies of Bennett linkages from four-crease origami patterns.

PubMed

Zhang, Xiao; Chen, Yan

2018-02-01

This paper deals with constructing mobile assemblies of Bennett linkages inspired by four-crease origami patterns. A transition technique has been proposed by taking the thick-panel form of an origami pattern as an intermediate bridge. A zero-thickness rigid origami pattern and its thick-panel form share the same sector angles and folding behaviours, while the thick-panel origami and the mobile assembly of linkages are kinematically equivalent with differences only in link profiles. Applying this transition technique to typical four-crease origami patterns, we have found that the Miura-ori and graded Miura-ori patterns lead to assemblies of Bennett linkages with identical link lengths. The supplementary-type origami patterns with different mountain-valley crease assignments correspond to different types of Bennett linkage assemblies with negative link lengths. And the identical linkage-type origami pattern generates a new mobile assembly. Hence, the transition technique offers a novel approach to constructing mobile assemblies of spatial linkages from origami patterns.

Improving the practicality and safety of artificial corneas: Pre-assembly and gamma-rays sterilization of the boston keratoprosthesis.

PubMed

Gonzalez-Andrades, Miguel; Sharifi, Roholah; Islam, Mohammad-Mirazul; Divoux, Thibaut; Haist, Michael; Paschalis, Eleftherios; Gelfand, Larisa; Mamodaly, Shamina; Di Cecilia, Luca; Cruzat, Andrea; Ulm, Franz-Josef; Chodosh, James; Delori, Francois; Dohlman, Claes H

2018-04-05

To make the Boston keratoprosthesis (B-KPro), together with its carrier corneal graft, more easily procured, transported and stored, as well as less expensive, easier for the surgeon to implant and safer for the patient, it is proposed that the B-KPro-graft combination be pre-assembled by an expert technician, followed by sterilization with gamma ray irradiation (GI) allowing long-term storage at room temperature. For this to be possible, it must be shown that the B-KPro itself (not only the graft) remains unharmed by the irradiation. Polymethyl methacrylate (PMMA) discs and B-KPros were submitted to either ethylene oxide sterilization or different doses of GI. Cell biocompatibility, mechanical strength and optical quality were evaluated. The feasibility of assembling the B-KPro to a corneal graft, and gamma-radiate afterwards, was also assessed. There were no differences in cell biocompatibility between the samples. The optical evaluation showed high levels of transparency for all the groups. The absorbance of ultraviolet was higher for the groups treated with GI. The mechanical evaluation by nanoindentation showed no alterations of the PMMA discs after GI. The flexure test revealed a similar mechanical behavior. Technically, pre-assembly and GI of the B-KPro revealed no problems. Sterilization of B-KPro using GI has no detrimental influence on the device. The pre-assembly of B-KPro to a donor cornea, followed by gamma sterilization, emerges as an efficient and safe procedure. Copyright © 2018. Published by Elsevier Inc.

36 CFR 1234.12 - What are the fire safety requirements that apply to records storage facilities?

Code of Federal Regulations, 2011 CFR

2011-07-01

... structural elements and construction assemblies must be in accordance with ASTM E 119-98 (incorporated by... necessary, they must be protected by self-closing or automatic Class A fire doors, or equivalent doors that... such areas with 4-hour rated construction assemblies. (p) Equipment rows running perpendicular to the...

Developing Form Assembly Specifications for Exams with Multiple Choice and Constructed Response Items: Balancing Reliability and Validity Concerns

ERIC Educational Resources Information Center

Hendrickson, Amy; Patterson, Brian; Ewing, Maureen

2010-01-01

The psychometric considerations and challenges associated with including constructed response items on tests are discussed along with how these issues affect the form assembly specifications for mixed-format exams. Reliability and validity, security and fairness, pretesting, content and skills coverage, test length and timing, weights, statistical…

Runner hub construction for propeller type turbine

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eyster, P.J.; Yanek, E.J.

1976-08-10

A runner hub is described for a propeller type hydraulic turbine wherein the hub is constructed of at least a pair of arcuate segments. When the arcuate segments are assembled together, they form a hollow hub. Turnbuckles are provided within the hub attached to opposite hub segments and are adjustable to forcibly hold the hub segments in the assembled position.

Construction of high resolution genetic linkage maps to improve the soybean genome sequence assembly Glyma1.01

USDA-ARS?s Scientific Manuscript database

A landmark in soybean research, Glyma1.01, the first whole genome sequence of variety Williams 82 (Glycine max L. Merr.) was completed in 2010 and is widely used. However, because the assembly was primarily built based on the linkage maps constructed with a limited number of markers and recombinant...

Development of assembly techniques for fire resistant aircraft interior panels

NASA Technical Reports Server (NTRS)

Lee, S. C. S.

1978-01-01

Ten NASA Type A fire resistant aircraft interior panels were fabricated and tested to develop assembly techniques. These techiques were used in the construction of a full scale lavatory test structure for flame propagation testing. The Type A panel is of sandwich construction consisting of Nomex honeycomb filled with quinone dioxime foam, and bismaleimide/glass face sheets bonded to the core with polyimide film adhesive. The materials selected and the assembly techniques developed for the lavatory test structure were designed for obtaining maximum fire containment with minimum smoke and toxic emission.

Analysis on the hot spot and trend of the foreign assembly building research

NASA Astrophysics Data System (ADS)

Bi, Xiaoqing; Luo, Yanbing

2017-03-01

First of all, the paper analyzes the research on the front of the assembly building in the past 15 years. This article mainly adopts the method of CO word analysis, construct the co word matrix, correlation matrix, and then into a dissimilarity matrix, and on this basis, using factor analysis, cluster analysis and multi scale analysis method to study the structure of prefabricated construction field display. Finally, the results of the analysis are discussed, and summarized the current research focus of foreign prefabricated construction mainly concentrated in 7 aspects: embankment construction, wood construction, bridge construction, crane layout, PCM wall and glass system, based on neural network test, energy saving and recycling, and forecast the future trend of development study.

A commercial project for private investments. Update of the 280 MW api Energia IGCC plant construction in central Italy.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Del Bravo, R.; Pinacci, P.; Trifilo, R.

1998-07-01

This paper has the aim to give a general overview of the api Energia IGCC project starting from the project background in 1992 and ending with the progress of construction. api Energia S.p.A., a joint VENTURE between api anonima petroli italiana S.p.A., Roma, Italy (51%), ABB Sae Sadelmi S.p.A., Milano, Italy (25%) and Texaco Development Corporation (24%), is building a 280 MW Integrated Gasification Combined Cycle plant in the api refinery at Falconara Marittima, on Italy' s Adriatic coast, using heavy oil residues. The plant is based on the modern concept of employing a highly efficient combined cycle power plantmore » fed with a low heating value fuel gas produced by gasifying heavy refinery residues. This scheme provides consistent advantages in terms of efficiency and environmental impact over alternative applications of the refinery residues. The electric power produced will feed the national grid. The project has been financed using the ``project financing'' scheme: over 1,000 billion Lira, representing 75% of the overall capital requirement, have been provided by a pool of international banks. In November 1996 the project reached financial closure and immediately after the detailed design and procurement activities started. Engineering, Procurement and Construction activities, carried out by a Consortium of companies of the ABB group, are totally in line with the schedule. Commercial operation of the plant, is scheduled for November 1999.« less

Assembly Modulated by Particle Position and Shape: A New Concept in Self-Assembly.

PubMed

Tavacoli, Joe W; Heuvingh, Julien; Du Roure, Olivia

2017-11-10

In this communication we outline how the bespoke arrangements and design of micron-sized superparamagnetic shapes provide levers to modulate their assembly under homogeneous magnetic fields. We label this new approach, 'assembly modulated by particle position and shape' (APPS). Specifically, using rectangular lattices of superparamagnetic micron-sized cuboids, we construct distinct microstructures by adjusting lattice pitch and angle of array with respect to a magnetic field. Broadly, we find two modes of assembly: (1) immediate 2D jamming of the cuboids as they rotate to align with the applied field (rotation-induced jamming) and (2) aggregation via translation after their full alignment (dipole-dipole assembly). The boundary between these two assembly pathways is independent on field strength being solely a function of the cuboid's dimensions, lattice pitch, and array angle with respect to field-a relationship which we capture, along with other features of the assembly process, in a 'phase diagram'. In doing so, we set out initial design rules to build custom made assemblies. Moreover, these assemblies can be made flexible thanks to the hinged contacts of their particle building blocks. This flexibility, combined with the superparamagnetic nature of the architectures, renders our assembly method particularly appropriate for the construction of complex actuators at a scale hitherto not possible.

Construction of Red Fox Chromosomal Fragments from the Short-Read Genome Assembly.

PubMed

Rando, Halie M; Farré, Marta; Robson, Michael P; Won, Naomi B; Johnson, Jennifer L; Buch, Ronak; Bastounes, Estelle R; Xiang, Xueyan; Feng, Shaohong; Liu, Shiping; Xiong, Zijun; Kim, Jaebum; Zhang, Guojie; Trut, Lyudmila N; Larkin, Denis M; Kukekova, Anna V

2018-06-20

The genome of a red fox ( Vulpes vulpes ) was recently sequenced and assembled using next-generation sequencing (NGS). The assembly is of high quality, with 94X coverage and a scaffold N50 of 11.8 Mbp, but is split into 676,878 scaffolds, some of which are likely to contain assembly errors. Fragmentation and misassembly hinder accurate gene prediction and downstream analysis such as the identification of loci under selection. Therefore, assembly of the genome into chromosome-scale fragments was an important step towards developing this genomic model. Scaffolds from the assembly were aligned to the dog reference genome and compared to the alignment of an outgroup genome (cat) against the dog to identify syntenic sequences among species. The program Reference-Assisted Chromosome Assembly (RACA) then integrated the comparative alignment with the mapping of the raw sequencing reads generated during assembly against the fox scaffolds. The 128 sequence fragments RACA assembled were compared to the fox meiotic linkage map to guide the construction of 40 chromosomal fragments. This computational approach to assembly was facilitated by prior research in comparative mammalian genomics, and the continued improvement of the red fox genome can in turn offer insight into canid and carnivore chromosome evolution. This assembly is also necessary for advancing genetic research in foxes and other canids.

Logistical Support for the Installation of the Plate Boundary Observatory GPS and Borehole Strainmeter Networks

NASA Astrophysics Data System (ADS)

Kurnik, C.; Austin, K.; Coyle, B.; Dittmann, T.; Feaux, K.; Friesen, B.; Johnson, W.; Mencin, D.; Pauk, B.; Walls, C.

2007-12-01

The Plate Boundary Observatory (PBO), part of the NSF-funded EarthScope project, is designed to study the three- dimensional strain field resulting from deformation across the active boundary zone between the Pacific and North American plates in the western United States. To meet these goals, UNAVCO will install 880 continuous GPS stations, 103 borehole strainmeter stations, 28 tiltmeters, and five laser strainmeters by October 2008. Such a broad network presents significant logisitical challenges, including moving supplies, equipment, and personnel around 6 million square kilometers, and this requires accurate tracking and careful planning. The PBO logistics chain includes the PBO headquarters at UNAVCO in Boulder, Colorado and five regional offices in the continental United States and Alaska, served by dozens of suppliers spread across the globe. These offices are responsible for building and maintaining sites in their region. Most equipment and supplies first arrive in Boulder, where they are tagged and entered into a UNAVCO-wide equipment database, assembled and quality checked as necessary, and sent on to the appropriate regional office. Larger items which are costly to store and ship from Boulder, such as batteries or long sections of stainless steel pipe and bar required for monuments, are shipped directly from the supplier to each region as needed. These supplies and equipment are also tracked through the ordering, delivery, installation, and maintenance cycle via Earned Value Management techniques which allow us to meet NSF and other Federal procurement rules. Early prototypes and assembly configurations aid the development of material and supply budgets. A thorough understanding of Federal procurement rules at project start up is critical as the project moves forward.

Title I preliminary engineering for: A. S. E. F. solid waste to methane gas

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1976-01-01

An assignment to provide preliminary engineering of an Advanced System Experimental Facility for production of methane gas from urban solid waste by anaerobic digestion is documented. The experimental facility will be constructed on a now-existing solid waste shredding and landfill facility in Pompano Beach, Florida. Information is included on: general description of the project; justification of basic need; process design; preliminary drawings; outline specifications; preliminary estimate of cost; and time schedules for design and construction of accomplishment of design and construction. The preliminary cost estimate for the design and construction phases of the experimental program is $2,960,000, based on Dec.more » 1975 and Jan. 1976 costs. A time schedule of eight months to complete the Detailed Design, Equipment Procurement and the Award of Subcontracts is given.« less

29 CFR 1926.1407 - Power line safety (up to 350 kV)-assembly and disassembly.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 8 2011-07-01 2011-07-01 false Power line safety (up to 350 kV)-assembly and disassembly... Cranes and Derricks in Construction § 1926.1407 Power line safety (up to 350 kV)—assembly and disassembly... area of assembly/disassembly, closer than 20 feet to a power line during the assembly/disassembly...

The cost risk implementation on design-build project of integrated public spaces child friendly in capital of Jakarta

NASA Astrophysics Data System (ADS)

Mardiaman, Mubarok, Abdul

2017-11-01

Jakarta area of 662.33 km2 with a population of 10,075,030 inhabitants and green open spaces 9.98%. The Jakarta government built a child-friendly integrated open space as facilities for playing. Providing of facilities was hoped suitable with time, cost, quality, accountability and proper financial governance. Based on the PU ministerial regulation number 19/PRT/M/2015 on the standards and guidelines for procurement the design and construction work on the integrated build and the PU ministerial regulation No. 07/PRT/M/2011 on standards and guidelines for procurement of construction works and consulting services of public works and the ministry of housing. RPTRA development at 123 locations in Jakarta was implemented base on the contract of design and build. The design study was influenced by the cost elements; the main strength (expert), skilled personnel, support personnel, major equipment and support. The construction fee relies on; expert implementation, hardware implementation, preparation work, land, buildings, courtyards, fences, complementary and governance capabilities for human resources in completing the construction activities to minimize the cost risk. Montecarlo simulations was conducted to determine the average unit price, model and analyze systems. In the cost contract, the percentage of design work stipulated 2.5%, build 97.5%. Base on regulation the minister of public work for design work cost 2.72%, build 97.28%. Then, actual cost for design 2.67% and build 97.33%. From the three reference was shown that there are differentiation one another. The acceleration of planning able to make the cost and time more efficient that impact on the implementation margin.

The weekend effect alters the procurement and discard rates of deceased donor kidneys in the United States

PubMed Central

Mohan, Sumit; Foley, Karl; Chiles, Mariana C; Dube, Geoffrey K; Patzer, Rachel E; Pastan, Stephen; Crew, R John; Cohen, David; Ratner, Lloyd

2016-01-01

Factors contributing to the high rate of discard among deceased donor kidneys remain poorly understood and the influence of resource limitations of weekends on kidney transplantation is unknown. To quantify this we used data from the Scientific Registry of Transplant Recipients and assembled a retrospective cohort of 181,799 deceased donor kidneys recovered for transplantation from 2000-2013. We identified the impact of the day of the week on the procurement and subsequent utilization or discard of deceased donor kidneys in the United States, as well as report the geographic variation on the impact of weekends on transplantation. Compared to weekday kidneys, organs procured on weekends were significantly more likely to be discarded than transplanted (odds ratio: 1.16; 95% confidence interval 1.13 – 1.19), even after adjusting for organ quality (adjusted odds ratio: 1.13; 95% confidence interval 1.10 – 1.17). Weekend discards were of a significantly higher quality than weekday discards (kidney donor profile index: 76.5 vs 77.3%). Considerable geographic variation was noted in the proportion of transplants that occurred over the weekend. Kidneys available for transplant over the weekend were significantly more likely to be used at larger transplant centers, be shared without payback, and experienced shorter cold ischemia times. Thus, factors other than kidney quality are contributing to the discard of deceased donor kidneys, particularly during weekends. Policy prescriptions, administrative or organizational solutions within transplant programs may potentially mitigate against the recent increase in kidney discards. PMID:27182001

Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Torella, JP; Lienert, F; Boehm, CR

2014-08-07

Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts, and they hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these pathways and circuits have become more complex, and the increasing need for standardization and insulation of genetic parts has resulted in sequence redundancies-for example, repeated terminator and insulator sequences-that complicate recombination-based assembly. We and others have recently developed DNA assembly methods, which we refer to collectively as unique nucleotide sequence (UNS)-guided assembly, in which individual DNA parts are flanked withmore » UNSs to facilitate the ordered, recombination-based assembly of repetitive sequences. Here we present a detailed protocol for UNS-guided assembly that enables researchers to convert multiple DNA parts into sequenced, correctly assembled constructs, or into high-quality combinatorial libraries in only 2-3 d. If the DNA parts must be generated from scratch, an additional 2-5 d are necessary. This protocol requires no specialized equipment and can easily be implemented by a student with experience in basic cloning techniques.« less

Unique nucleotide sequence (UNS)-guided assembly of repetitive DNA parts for synthetic biology applications

PubMed Central

Torella, Joseph P.; Lienert, Florian; Boehm, Christian R.; Chen, Jan-Hung; Way, Jeffrey C.; Silver, Pamela A.

2016-01-01

Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts and hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these pathways and circuits have become more complex, and the increasing need for standardization and insulation of genetic parts has resulted in sequence redundancies — for example repeated terminator and insulator sequences — that complicate recombination-based assembly. We and others have recently developed DNA assembly methods that we refer to collectively as unique nucleotide sequence (UNS)-guided assembly, in which individual DNA parts are flanked with UNSs to facilitate the ordered, recombination-based assembly of repetitive sequences. Here we present a detailed protocol for UNS-guided assembly that enables researchers to convert multiple DNA parts into sequenced, correctly-assembled constructs, or into high-quality combinatorial libraries in only 2–3 days. If the DNA parts must be generated from scratch, an additional 2–5 days are necessary. This protocol requires no specialized equipment and can easily be implemented by a student with experience in basic cloning techniques. PMID:25101822

Flexture plate motion-transfer mechanism, beam-splitter assembly, and interferometer incorporating the same

DOEpatents

Carangelo, R.M.; Dettori, M.D.; Grigely, L.J.; Murray, T.C.; Solomon, P.R.; Dine, C.P. Van; Wright, D.D.

1996-01-23

A multiplicity of one-piece flexure plates are assembled in pairs to provide a support system on which a retroreflector may be mounted for reciprocal motion. Combined with balance bodies, the flexure plates provide a support system having portions that are dynamically and statically balanced with one another, irrespective of orientation, so as to thereby immunize the unit against extraneous forces. The motion transfer assembly is especially adapted for use to support a moving retroreflector in a two-arm interferometer that may further include a beamsplitter assembly constructed from a one-piece, integrally formed body, the body having convergent, optically flat planar surfaces of specular reflectance, and means for adjustably mounting a beamsplitter therein. The spectrometer is of modular construction, and employs an integrated clocking sub-assembly as well as a light-weight voice-coil motor. 15 figs.

Flexture plate motion-transfer mechanism, beam-splitter assembly, and interferometer incorporating the same

DOEpatents

Carangelo, Robert M.; Dettori, Mark D.; Grigely, Lawrence J.; Murray, Terence C.; Solomon, Peter R.; Van Dine, C. Peter; Wright, David D.

1996-01-01

A multiplicity of one-piece flexure plates are assembled in pairs to provide a support system on which a retroreflector may be mounted for reciprocal motion. Combined with balance bodies, the flexure plates provide a support system having portions that are dynamically and statically balanced with one another, irrespective of orientation, so as to thereby immunize the unit against extraneous forces. The motion transfer assembly is especially adapted for use to support a moving retroreflector in a two-arm interferometer that may further include a beamsplitter assembly constructed from a one-piece, integrally formed body, the body having convergent, optically flat planar surfaces of specular reflectance, and means for adjustably mounting a beamsplitter therein. The spectrometer is of modular construction, and employs an integrated clocking sub-assembly as well as a light-weight voice-coil motor.

5. CONSTRUCTION PROGRESS VIEW OF ASSEMBLY USED TO RAISE AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. CONSTRUCTION PROGRESS VIEW OF ASSEMBLY USED TO RAISE AND LOWER FUEL ELEMENTS. TAKEN FROM TOP OF SHIELDING TANK WITH CAMERA POINTING TOWARDS BOTTOM OF TANK. SHOWS LADDER, SQUARE LIFTING FRAME, FUEL ELEMENT HOLDERS, AND CABLE CYLINDERS. INEL PHOTO NUMBER 65-5434, TAKEN OCTOBER 20, 1965. - Idaho National Engineering Laboratory, Advanced Reentry Vehicle Fusing System, Scoville, Butte County, ID

Construction and Self-Assembly of Single-Chain Polymer Nanoparticles via Coordination Association and Electrostatic Repulsion in Water.

PubMed

Zhu, Zhengguang; Xu, Na; Yu, Qiuping; Guo, Lei; Cao, Hui; Lu, Xinhua; Cai, Yuanli

2015-08-01

Simultaneous coordination-association and electrostatic-repulsion interactions play critical roles in the construction and stabilization of enzymatic function metal centers in water media. These interactions are promising for construction and self-assembly of artificial aqueous polymer single-chain nanoparticles (SCNPs). Herein, the construction and self-assembly of dative-bonded aqueous SCNPs are reported via simultaneous coordination-association and electrostatic-repulsion interactions within single chains of histamine-based hydrophilic block copolymer. The electrostatic-repulsion interactions are tunable through adjusting the imidazolium/imidazole ratio in response to pH, and in situ Cu(II)-coordination leads to the intramolecular association and single-chain collapse in acidic water. SCNPs are stabilized by the electrostatic repulsion of dative-bonded block and steric shielding of nonionic water-soluble block, and have a huge specific surface area of function metal centers accessible to substrates in acidic water. Moreover, SCNPs can assemble into micelles, networks, and large particles programmably in response to the solution pH. These unique media-sensitive phase-transformation behaviors provide a general, facile, and versatile platform for the fabrication of enzyme-inspired smart aqueous catalysts. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Direct procurement of wound management products.

PubMed

Jenkins, Trevor

2014-03-01

This article describes a collaborative project between Bedfordshire Community Health Services and Primary Care Trusts/Clinical Commissioning Groups to improve provision of dressings to nurses for the patients they treat. Commissioners have facilitated a transformational approach and encouraged development of efficient systems of increased cost-effectiveness rather than a transactional approach based on opportunistic cost improvement plans. Reconfiguration to direct procurement from GP prescribing has reduced wastage, released nurse time from processes to spend on clinical contact time with patients, increased efficiency, and reduced prescription workload for GPs, all without adverse effects on expenditure. Establishing a wound care products formulary placed control under the nurses treating patients and facilitated decision-making based on cost-effectiveness in clinical use. Nurses now manage 60% of expenditure in the local community health economy, and this is increasing. Relationships with the dressings manufacturing industry have also changed in a positive, constructive direction.

Targeted self-assembly of functionalized carbon nanotubes on tumors

DOEpatents

Scheinberg, David A.; McDevitt, Michael R.; Villa, Carlos H.; Mulvey, J. Justin

2018-05-22

Provided herein are methods for delivering a molecule in situ to a cell and for treating a cancer via the in situ delivery. The methods comprise contacting or administering to the cell, as two separate components, a morpholino oligonucleotide comprising a targeting moiety followed by a single wall nanotube construct comprising second morpholino oligonucleotides complementary to the first morpholino oligonucleotides and one or both of a therapeutic or diagnostic payload molecule linked to the single wall nanotube construct. Upon self-assembly of a single wall nanotube complex via hybridization of the first morpholino and second complementary morpholino oligonucleotides at the cell, the payload molecule is delivered. Also provided is the two component self-assembly single wall nanotube system and the single wall nanotube construct comprising the second component.

One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy

PubMed Central

Casini, Arturo; MacDonald, James T.; Jonghe, Joachim De; Christodoulou, Georgia; Freemont, Paul S.; Baldwin, Geoff S.; Ellis, Tom

2014-01-01

Overlap-directed DNA assembly methods allow multiple DNA parts to be assembled together in one reaction. These methods, which rely on sequence homology between the ends of DNA parts, have become widely adopted in synthetic biology, despite being incompatible with a key principle of engineering: modularity. To answer this, we present MODAL: a Modular Overlap-Directed Assembly with Linkers strategy that brings modularity to overlap-directed methods, allowing assembly of an initial set of DNA parts into a variety of arrangements in one-pot reactions. MODAL is accompanied by a custom software tool that designs overlap linkers to guide assembly, allowing parts to be assembled in any specified order and orientation. The in silico design of synthetic orthogonal overlapping junctions allows for much greater efficiency in DNA assembly for a variety of different methods compared with using non-designed sequence. In tests with three different assembly technologies, the MODAL strategy gives assembly of both yeast and bacterial plasmids, composed of up to five DNA parts in the kilobase range with efficiencies of between 75 and 100%. It also seamlessly allows mutagenesis to be performed on any specified DNA parts during the process, allowing the one-step creation of construct libraries valuable for synthetic biology applications. PMID:24153110

Buffer for a gamma-insensitive optical sensor with gas and a buffer assembly

DOEpatents

Kruger, Hans W.

1994-01-01

A buffer assembly for a gamma-insensitive gas avalanche focal plane array operating in the ultra-violet/visible/infrared energy wavelengths and using a photocathode and an avalanche gas located in a gap between an anode and the photocathode. The buffer assembly functions to eliminate chemical compatibility between the gas composition and the materials of the photocathode. The buffer assembly in the described embodiment is composed of two sections, a first section constructed of glass honeycomb under vacuum and a second section defining a thin barrier film or membrane constructed, for example, of Al and Be, which is attached to and supported by the honeycomb. The honeycomb section, in turn, is supported by and adjacent to the photocathode.

Deviation diagnosis and analysis of hull flat block assembly based on a state space model

NASA Astrophysics Data System (ADS)

Zhang, Zhiying; Dai, Yinfang; Li, Zhen

2012-09-01

Dimensional control is one of the most important challenges in the shipbuilding industry. In order to predict assembly dimensional variation in hull flat block construction, a variation stream model based on state space was presented in this paper which can be further applied to accuracy control in shipbuilding. Part accumulative error, locating error, and welding deformation were taken into consideration in this model, and variation propagation mechanisms and the accumulative rule in the assembly process were analyzed. Then, a model was developed to describe the variation propagation throughout the assembly process. Finally, an example of flat block construction from an actual shipyard was given. The result shows that this method is effective and useful.

Buffer for a gamma-insensitive optical sensor with gas and a buffer assembly

DOEpatents

Kruger, H.W.

1994-05-10

A buffer assembly is disclosed for a gamma-insensitive gas avalanche focal plane array operating in the ultra-violet/visible/infrared energy wavelengths and using a photocathode and an avalanche gas located in a gap between an anode and the photocathode. The buffer assembly functions to eliminate chemical compatibility between the gas composition and the materials of the photocathode. The buffer assembly in the described embodiment is composed of two sections, a first section constructed of glass honeycomb under vacuum and a second section defining a thin barrier film or membrane constructed, for example, of Al and Be, which is attached to and supported by the honeycomb. The honeycomb section, in turn, is supported by and adjacent to the photocathode. 7 figures.

Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker.

PubMed

Mo, Xuejun; Li, Qiushi; Yi Lui, Lena Wai; Zheng, Baixue; Kang, Chiang Huen; Nugraha, Bramasta; Yue, Zhilian; Jia, Rui Rui; Fu, Hong Xia; Choudhury, Deepak; Arooz, Talha; Yan, Jie; Lim, Chwee Teck; Shen, Shali; Hong Tan, Choon; Yu, Hanry

2010-10-01

Tissue constructs that mimic the in vivo cell-cell and cell-matrix interactions are especially useful for applications involving the cell- dense and matrix- poor internal organs. Rapid and precise arrangement of cells into functional tissue constructs remains a challenge in tissue engineering. We demonstrate rapid assembly of C3A cells into multi- cell structures using a dendrimeric intercellular linker. The linker is composed of oleyl- polyethylene glycol (PEG) derivatives conjugated to a 16 arms- polypropylenimine hexadecaamine (DAB) dendrimer. The positively charged multivalent dendrimer concentrates the linker onto the negatively charged cell surface to facilitate efficient insertion of the hydrophobic oleyl groups into the cellular membrane. Bringing linker- treated cells into close proximity to each other via mechanical means such as centrifugation and micromanipulation enables their rapid assembly into multi- cellular structures within minutes. The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the constructs. We constructed defined multi- cellular structures such as rings, sheets or branching rods that can serve as potential tissue building blocks to be further assembled into complex 3D tissue constructs for biomedical applications. 2010 Elsevier Ltd. All rights reserved.

Astronaut Ross Approaches Assembly Concept for Construction of Erectable Space Structure (ACCESS)

NASA Technical Reports Server (NTRS)

1999-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Ross, perched on the Manipulator Foot Restraint (MFR) approaches the erected ACCESS. The primary objective of these experiments was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

STS-61B Astronaut Ross Works on Assembly Concept for Construction of Erectable Space Structure

NASA Technical Reports Server (NTRS)

1985-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo astronaut Ross, located on the Manipulator Foot Restraint (MFR) over the cargo bay, erects ACCESS. The primary objective of this experiment was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Ross Works on the Assembly Concept for Construction of Erectable Space Structure (ACCESS) During

NASA Technical Reports Server (NTRS)

1985-01-01

The crew assigned to the STS-61B mission included Bryan D. O'Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission's primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Ross works on ACCESS high above the orbiter. The primary objective of these experiments was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Benchmarking organ procurement organizations: a national study.

PubMed Central

Ozcan, Y A; Begun, J W; McKinney, M M

1999-01-01

OBJECTIVE: An exploratory examination of the technical efficiency of organ procurement organizations (OPOs) relative to optimal patterns of production in the population of OPOs in the United States. DATA SOURCES: A composite data set with the OPO as the unit of analysis, constructed from a 1995 national survey of OPOs (n = 64), plus secondary data from the Association of Organ Procurement Organizations and the United Network for Organ Sharing. STUDY DESIGN: The study uses data envelopment analysis (DEA) to evaluate the technical efficiency of all OPOs. PRINCIPAL FINDINGS: Overall, six of the 22 larger OPOs (27 percent) are classified as inefficient, while 23 of the 42 smaller OPOs (55 percent) are classified as inefficient. Efficient OPOs recover significantly more kidneys and extrarenal organs; have higher operating expenses; and have more referrals, donors, extrarenal transplants, and kidney transplants. The quantities of hospital development personnel and other personnel, and formalization of hospital development activities in both small and large OPOs, do not significantly differ. CONCLUSIONS: Indications that larger OPOs are able to operate more efficiently relative to their peers suggest that smaller OPOs are more likely to benefit from technical assistance. More detailed information on the activities of OPO staff would help pinpoint activities that can increase OPO efficiency and referrals, and potentially improve outcomes for large numbers of patients awaiting transplants. PMID:10536974

Assembly of filopodia by the formin FRL2 (FMNL3)

PubMed Central

Harris, Elizabeth S.; Gauvin, Timothy J.; Heimsath, Ernest G.; Higgs, Henry N.

2010-01-01

Actin-dependent finger-like protrusions such as filopodia and microvilli are widespread in eukaryotes, but their assembly mechanisms are poorly understood. Filopodia assembly requires at least three biochemical activities on actin: actin filament nucleation, prolonged actin filament elongation, and actin filament bundling. These activities are shared by several mammalian formin proteins, including mDia2, FRL1 (also called FMNL1), and FRL2 (FMNL3). In this paper, we compare the abilities of constructs from these three formins to induce filopodia. FH1-FH2 constructs of both FRL2 and mDia2 stimulate potent filopodia assembly in multiple cell types, and enrich strongly at filopodia tips. In contrast, FRL1 FH1-FH2 lacks this activity, despite possessing similar biochemical activities and being highly homologous to FRL2. Chimeric FH1-FH2 experiments between FRL1 and FRL2 show that, while both an FH1 and an FH2 are needed, either FH1 domain supports filopodia assembly but only FRL2’s FH2 domain allows this activity. A mutation that compromises FRL2’s barbed end binding ability abolishes filopodia assembly. FRL2’s ability to stimulate filopodia assembly is not altered by additional domains (GBD, DID, DAD), but is significantly reduced in the full-length construct, suggesting that FRL2 is subject to inhibitory regulation. The data suggest that the FH2 domain of FRL2 possesses properties not shared by FRL1 that allow it to generate filopodia. PMID:20862687

Special Operations Forces: Opportunities Exist to Improve Transparency of Funding and Assess Potential to Lessen Some Deployments

DTIC Science & Technology

2015-07-01

pace of special operations deployments, but opportunities may exist to better balance the workload across the joint force because activities...executes funding in operation and maintenance; procurement; research , development, test, and evaluation; and military construction accounts.13 SOCOM...regional awareness. Moreover, officials noted that increases in civilian positions were driven partly by DOD’s attempts to rebalance workload and become a

Department of the Army Justification of Estimates for Fiscal Years 1988/1989. Procurement/Appropriations-Construction Program Submitted to Congress.

DTIC Science & Technology

1987-01-01

e4run stoag :ahbl’ -ss to -ervice the, IDS equipment a-nd1 tho liFr . F)- fencing ;haill bo prov.id-d iroun,! the IDS area with necessary gats nd roa1d I...will provide enhanced and expanded capabilities. The EMCS (with 2,000 points) will control package boilers at 24 locations, one furnace, chiller

A Systems Approach to Military Construction.

DTIC Science & Technology

1982-11-01

Unclassi fled I150. OECL ASSI F1C ATI ON/ DOWNGRADING I SCHEDULE IS. DISYR1EUTION STATEMENT (ot this Repo"t) Approved for public release; distribution...30 Procurement Alternatives 30 Design Alternatives 33 Preconcept Control Data 34 AE Selection Procedure 36 Scheduling 40 Cost Estimating 44 4...data, scheduling , and cost estimating. The objectives of project coordination for a systems-oriented project do not differ from those of a

Stakeholder Alignment for Requirements in GEOValue

NASA Astrophysics Data System (ADS)

Cutcher-Gershenfeld, J.; King, J. L.

2016-12-01

Observation systems that collect information on environmental parameters relevant to biological and physical earth resources provide value. This has been demonstrated so many times in so many ways that it is not worth deliberating. Earlier projects for research (whether it is possible to do this or that), or for "dual use," typically involving defense, have been successful. Wealthy parties have built their own systems. Less wealthy parties seek to sustain the systems they have built. The history of systems suggests that "requirements" will be the next step. The objective is to maximize the "return" on the substantial investment required for construction, deployment, maintenance and renewal of observation systems. Stakeholders and their interests are assessed to construct the requirements from which specifications are built. Specifications drive procurement, and procurement produces built systems. Complicated (e.g. space-based) systems have long times between requirements analysis and deployment. It all depends on getting the requirements right, which depends on understanding stakeholders and requirements. And this is where things get complicated. Stakeholders and interests change, sometimes rapidly, as what is possible is altered. It becomes increasingly difficult to achieve stakeholder alignment required for effective management of constituent politics at the heart of any expensive endeavor. This paper presents results from a major study of stakeholder alignment in the Earth Sciences, focused especially on EarthCube.

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

PubMed Central

Richardson, Ruth E.; Suzuki, Yo

2015-01-01

Numerous DNA assembly technologies exist for generating plasmids for biological studies. Many procedures require complex in vitro or in vivo assembly reactions followed by plasmid propagation in recombination-impaired Escherichia coli strains such as DH5α, which are optimal for stable amplification of the DNA materials. Here we show that despite its utility as a cloning strain, DH5α retains sufficient recombinase activity to assemble up to six double-stranded DNA fragments ranging in size from 150 bp to at least 7 kb into plasmids in vivo. This process also requires surprisingly small amounts of DNA, potentially obviating the need for upstream assembly processes associated with most common applications of DNA assembly. We demonstrate the application of this process in cloning of various DNA fragments including synthetic genes, preparation of knockout constructs, and incorporation of guide RNA sequences in constructs for clustered regularly interspaced short palindromic repeats (CRISPR) genome editing. This consolidated process for assembly and amplification in a widely available strain of E. coli may enable productivity gain across disciplines involving recombinant DNA work. PMID:26348330

Variable Cost to Procure

DTIC Science & Technology

1981-03-12

Procurement Work Directive ( PWV ) Procurement Army, CostL to Procure Cost Differential Secondary (PAS) Cost to Buy Economic Order Quantity Procurement...and Assignment of Buyer h. Solicitation Fffort . ) (1) Procurement Planning ( ) ( ) (?) PWV Review and Small Business Coordination ( (3) Determination

Subsystem Hazard Analysis Methodology for the Ares I Upper Stage Source Controlled Items

NASA Technical Reports Server (NTRS)

Mitchell, Michael S.; Winner, David R.

2010-01-01

This article describes processes involved in developing subsystem hazard analyses for Source Controlled Items (SCI), specific components, sub-assemblies, and/or piece parts, of the NASA ARES I Upper Stage (US) project. SCIs will be designed, developed and /or procured by Boeing as an end item or an off-the-shelf item. Objectives include explaining the methodology, tools, stakeholders and products involved in development of these hazard analyses. Progress made and further challenges in identifying potential subsystem hazards are also provided in an effort to assist the System Safety community in understanding one part of the ARES I Upper Stage project.

Space Construction Automated Fabrication Experiment Definition Study (SCAFEDS). Volume 1: Executive summary

NASA Technical Reports Server (NTRS)

1978-01-01

The techniques, processes, and equipment required for automatic fabrication and assembly of structural elements in space using the space shuttle as a launch vehicle and construction base were investigated. Additional construction/systems/operational techniques, processes, and equipment which can be developed/demonstrated in the same program to provide further risk reduction benefits to future large space systems were included. Results in the areas of structure/materials, fabrication systems (beam builder, assembly jig, and avionics/controls), mission integration, and programmatics are summarized. Conclusions and recommendations are given.

Self-assembling multimeric nucleic acid constructs

DOEpatents

Cantor, Charles R.; Niemeyer, Christof M.; Smith, Cassandra L.; Sano, Takeshi; Hnatowich, Donald J.; Rusckowski, Mary

1999-10-12

The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

Self-assembling multimeric nucleic acid constructs

DOEpatents

Cantor, Charles R.; Niemeyer, Christof M.; Smith, Cassandra L.; Sano, Takeshi; Hnatowich, Donald J.; Rusckowski, Mary

1996-01-01

The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.

Self-assembling multimeric nucleic acid constructs

DOEpatents

Cantor, C.R.; Niemeyer, C.M.; Smith, C.L.; Sano, Takeshi; Hnatowich, D.J.; Rusckowski, M.

1996-10-01

The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products. 5 figs.

Recrystallization-induced self-assembly for the growth of Cu₂O superstructures.

PubMed

Shang, Yang; Shao, Yi-Ming; Zhang, Dong-Feng; Guo, Lin

2014-10-20

The assembly of inorganic nanoparticles (NPs) into 3D superstructures with defined morphologies is of particular interest. A novel strategy that is based on recrystallization-induced self-assembly (RISA) for the construction of 3D Cu2O superstructures and employs Cu2O mesoporous spheres with diameters of approximately 300 nm as the building blocks has now been developed. Balancing the hydrolysis and recrystallization rates of the CuCl precursors through precisely adjusting the experimental parameters was key to success. Furthermore, the geometry of the superstructures can be tuned to obtain either cubes or tetrahedra and was shown to be dependent on the growth behavior of bulk CuCl. The overall strategy extends the applicability of recrystallization-based processes for the guided construction of assemblies and offers unique insights for assembling larger particles into complicated 3D superstructures. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Lightweight Passive Microclimate Cooling Device

DTIC Science & Technology

1993-03-01

rabrication. No consideration was given to weight. We have examined alternate methods of construction of the backpack and cylinder assembly and thin...13 Figure 7. Water Adsorption on Magnesium Chloride ........... 14 Figure 8. Cylinder Assembly ................................ 20...Figure 9. Backpack Assembly ............................... 21 Figure 10. Unwrapped Vest ................................... 22 Figure 11. Adsorption

Construction and manipulation of functional three-dimensional droplet networks.

PubMed

Wauer, Tobias; Gerlach, Holger; Mantri, Shiksha; Hill, Jamie; Bayley, Hagan; Sapra, K Tanuj

2014-01-28

Previously, we reported the manual assembly of lipid-coated aqueous droplets in oil to form two-dimensional (2D) networks in which the droplets are connected through single lipid bilayers. Here we assemble lipid-coated droplets in robust, freestanding 3D geometries: for example, a 14-droplet pyramidal assembly. The networks are designed, and each droplet is placed in a designated position. When protein pores are inserted in the bilayers between specific constituent droplets, electrical and chemical communication pathways are generated. We further describe an improved means to construct 3D droplet networks with defined organizations by the manipulation of aqueous droplets containing encapsulated magnetic beads. The droplets are maneuvered in a magnetic field to form simple construction modules, which are then used to form larger 2D and 3D structures including a 10-droplet pyramid. A methodology to construct freestanding, functional 3D droplet networks is an important step toward the programmed and automated manufacture of synthetic minimal tissues.

Impact of highway construction on water bodies: a geospatial assessment.

PubMed

Vijay, Ritesh; Kushwaha, Vikash K; Mardikar, Trupti; Labhasetwar, P K

2017-08-01

India has witnessed a massive infrastructure boom in the past few years. One of such projects is National Highway-7 (NH-7), a North-South highway connecting Kanyakumari, Tamil Nadu, to Varanasi, Uttar Pradesh, traversing many water bodies. The present study aims to assess the pre- and post-construction impact due to existing, new and widened NH-7 on the physical status of the water bodies, using remote sensing techniques. Satellite images spanning 22 years were procured and analysed for change detection in land use and land cover within the waterbodies. The study indicates that construction activities have led to transformation within the water bodies regarding reduction in area and inter-changing of land use and land cover classes, in turn leading to siltation and reduction of recharge.

Assembly of hydrogel units for 3D microenvironment in a poly(dimethylsiloxane) channel

NASA Astrophysics Data System (ADS)

Cho, Chang Hyun; Kwon, Seyong; Park, Je-Kyun

2017-12-01

Construction of three-dimensional (3D) microenvironment become an important issue in recent biological studies due to their biological relevance compared to conventional two-dimensional (2D) microenvironment. Various fabrication techniques have been employed to construct a 3D microenvironment, however, it is difficult to fully satisfy the biological and mechanical properties required for the 3D cell culture system, such as heterogeneous tissue structures generated from the functional differences or diseases. We propose here an assembly method for facile construction of 3D microenvironment in a poly(dimethylsiloxane) (PDMS) channel using hydrogel units. The high-aspect-ratio of hydrogel units was achieved by fabricating these units using a 2D mold. With this approach, 3D heterogeneous hydrogel units were produced and assembled in a PDMS channel by structural hookup. In vivo-like 3D heterogeneous microenvironment in a precisely controllable fluidic system was also demonstrated using a controlled assembly of different types of hydrogel units, which was difficult to obtain from previous methods. By regulating the flow condition, the mechanical stability of the assembled hydrogel units was verified by the flow-induced deformation of hydrogel units. In addition, in vivo-like cell culture environment was demonstrated using an assembly of cell-coated hydrogel units in the fluidic channel. Based on these features, our method expects to provide a beneficial tool for the 3D cell culture module and biomimetic engineering.

Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly.

PubMed

Parfenov, Vladislav A; Koudan, Elizaveta V; Bulanova, Elena A; Karalkin, Pavel A; Pereira, Frederico DAS; Norkin, Nikita E; Knyazeva, Alisa D; Gryadunova, Anna A; Petrov, Oleg F; Vasiliev, M M; Myasnikov, Maxim; Chernikov, Valery P; Kasyanov, Vladimir A; Marchenkov, Artem Yu; Brakke, Kenneth A; Khesuani, Yusef D; Demirci, Utkan; Mironov, Vladimir A

2018-05-31

Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first-time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology. Label-free magnetic levitation was performed using a prototype device equipped with permanent magnets in presence of gadolinium (Gd3+) in culture media, which enables magnetic levitation. Mathematical modeling and computer simulations were used for prediction of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. First, we used polystyrene beads to simulate the assembly of tissue spheroids and to determine the optimal settings for magnetic levitation in presence of Gd3+. Second, we proved the ability of chondrospheres to assemble rapidly into 3D tissue construct in the permanent magnetic field in the presence of Gd3+. Thus, scaffold- and label-free magnetic levitation of tissue spheroids is a promising approach for rapid 3D biofabrication and attractive alternative to label-based magnetic force-driven tissue engineering. . © 2018 IOP Publishing Ltd.

Cloning-independent plasmid construction for genetic studies in streptococci

PubMed Central

Xie, Zhoujie; Qi, Fengxia; Merritt, Justin

2013-01-01

Shuttle plasmids are among the few routinely utilized tools in the Streptococcus mutans genetic system that still require the use of classical cloning methodologies and intermediate hosts for genetic manipulation. Accordingly, it typically requires considerably less time and effort to introduce mutations onto the S. mutans chromosome than it does to construct shuttle vectors for expressing genes in trans. Occasionally, shuttle vector constructs also exhibit toxicity in E. coli, which prevents their proper assembly. To circumvent these limitations, we modified a prolonged overlap extension PCR (POE-PCR) protocol to facilitate direct plasmid assembly in S. mutans. Using solely PCR, we created the reporter vector pZX7, which contains a single minimal streptococcal replication origin and harbors a spectinomycin resistance cassette and the gusA gene encoding β-glucuronidase. We compared the efficiency of pZX7 assembly using multiple strains of S. mutans and were able to obtain from 5×103 – 2×105 CFU/μg PCR product. Likewise, we used pZX7 to further demonstrate that Streptococcus sanguinis and Streptococcus gordonii are also excellent hosts for cloning-independent plasmid assembly, which suggests that this system is likely to function in numerous other streptococci. Consequently, it should be possible to completely forgo the use of E. coli – Streptococcus shuttle vectors in many streptococcal species, thereby decreasing the time and effort required to assemble constructs and eliminating any toxicity issues associated with intermediate hosts. PMID:23673081

Cloning-independent plasmid construction for genetic studies in streptococci.

PubMed

Xie, Zhoujie; Qi, Fengxia; Merritt, Justin

2013-08-01

Shuttle plasmids are among the few routinely utilized tools in the Streptococcus mutans genetic system that still require the use of classical cloning methodologies and intermediate hosts for genetic manipulation. Accordingly, it typically requires considerably less time and effort to introduce mutations onto the S. mutans chromosome than it does to construct shuttle vectors for expressing genes in trans. Occasionally, shuttle vector constructs also exhibit toxicity in Escherichia coli, which prevents their proper assembly. To circumvent these limitations, we modified a prolonged overlap extension PCR (POE-PCR) protocol to facilitate direct plasmid assembly in S. mutans. Using solely PCR, we created the reporter vector pZX7, which contains a single minimal streptococcal replication origin and harbors a spectinomycin resistance cassette and the gusA gene encoding β-glucuronidase. We compared the efficiency of pZX7 assembly using multiple strains of S. mutans and were able to obtain from 5 × 10³ to 2 × 10⁵ CFU/μg PCR product. Likewise, we used pZX7 to further demonstrate that Streptococcus sanguinis and Streptococcus gordonii are also excellent hosts for cloning-independent plasmid assembly, which suggests that this system is likely to function in numerous other streptococci. Consequently, it should be possible to completely forgo the use of E. coli-Streptococcus shuttle vectors in many streptococcal species, thereby decreasing the time and effort required to assemble constructs and eliminating any toxicity issues associated with intermediate hosts. Copyright © 2013 Elsevier B.V. All rights reserved.

Construction of energy transfer pathways self-assembled from DNA-templated stacks of anthracene.

PubMed

Iwaura, Rika; Yui, Hiroharu; Someya, Yuu; Ohnishi-Kameyama, Mayumi

2014-01-05

We describe optical properties of anthracene stacks formed from single-component self-assembly of thymidylic acid-appended anthracene 2,6-bis[5-(3'-thymidylic acid)pentyloxy] anthracene (TACT) and the binary self-assembly of TACT and complementary 20-meric oligoadenylic acid (TACT/dA20) in an aqueous buffer. UV-Vis and emission spectra for the single-component self-assembly of TACT and the binary self-assembly of TACT/dA20 were very consistent with stacked acene moieties in both self-assemblies. Interestingly, time-resolved fluorescence spectra from anthracene stacks exhibited very different features of the single-component and binary self-assemblies. In the single-component self-assembly of TACT, a dynamic Stokes shift (DSS) and relatively short fluorescence lifetime (τ=0.35ns) observed at around 450nm suggested that the anthracene moieties were flexible. Moreover, a broad emission at 530nm suggested the formation of an excited dimer (excimer). In the binary self-assembly of TACT/dA20, we detected a broad, red-shifted emission component at 534nm with a lifetime (τ=0.4ns) shorter than that observed in the TACT single-component self-assembly. Combining these results with the emission spectrum of the binary self-assembly of TACT/5'-HEX dA20, we concluded that the energy transfer pathway was constructed by columnar anthracene stacks formed from the DNA-templated self-assembly of TACT. Copyright © 2013 Elsevier B.V. All rights reserved.

Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants.

PubMed

Lukan, Tjaša; Machens, Fabian; Coll, Anna; Baebler, Špela; Messerschmidt, Katrin; Gruden, Kristina

2018-01-01

Cloning multiple DNA fragments for delivery of several genes of interest into the plant genome is one of the main technological challenges in plant synthetic biology. Despite several modular assembly methods developed in recent years, the plant biotechnology community has not widely adopted them yet, probably due to the lack of appropriate vectors and software tools. Here we present Plant X-tender, an extension of the highly efficient, scar-free and sequence-independent multigene assembly strategy AssemblX, based on overlap-depended cloning methods and rare-cutting restriction enzymes. Plant X-tender consists of a set of plant expression vectors and the protocols for most efficient cloning into the novel vector set needed for plant expression and thus introduces advantages of AssemblX into plant synthetic biology. The novel vector set covers different backbones and selection markers to allow full design flexibility. We have included ccdB counterselection, thereby allowing the transfer of multigene constructs into the novel vector set in a straightforward and highly efficient way. Vectors are available as empty backbones and are fully flexible regarding the orientation of expression cassettes and addition of linkers between them, if required. We optimised the assembly and subcloning protocol by testing different scar-less assembly approaches: the noncommercial SLiCE and TAR methods and the commercial Gibson assembly and NEBuilder HiFi DNA assembly kits. Plant X-tender was applicable even in combination with low efficient homemade chemically competent or electrocompetent Escherichia coli. We have further validated the developed procedure for plant protein expression by cloning two cassettes into the newly developed vectors and subsequently transferred them to Nicotiana benthamiana in a transient expression setup. Thereby we show that multigene constructs can be delivered into plant cells in a streamlined and highly efficient way. Our results will support faster introduction of synthetic biology into plant science.

Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants

PubMed Central

Machens, Fabian; Coll, Anna; Baebler, Špela; Messerschmidt, Katrin; Gruden, Kristina

2018-01-01

Cloning multiple DNA fragments for delivery of several genes of interest into the plant genome is one of the main technological challenges in plant synthetic biology. Despite several modular assembly methods developed in recent years, the plant biotechnology community has not widely adopted them yet, probably due to the lack of appropriate vectors and software tools. Here we present Plant X-tender, an extension of the highly efficient, scar-free and sequence-independent multigene assembly strategy AssemblX, based on overlap-depended cloning methods and rare-cutting restriction enzymes. Plant X-tender consists of a set of plant expression vectors and the protocols for most efficient cloning into the novel vector set needed for plant expression and thus introduces advantages of AssemblX into plant synthetic biology. The novel vector set covers different backbones and selection markers to allow full design flexibility. We have included ccdB counterselection, thereby allowing the transfer of multigene constructs into the novel vector set in a straightforward and highly efficient way. Vectors are available as empty backbones and are fully flexible regarding the orientation of expression cassettes and addition of linkers between them, if required. We optimised the assembly and subcloning protocol by testing different scar-less assembly approaches: the noncommercial SLiCE and TAR methods and the commercial Gibson assembly and NEBuilder HiFi DNA assembly kits. Plant X-tender was applicable even in combination with low efficient homemade chemically competent or electrocompetent Escherichia coli. We have further validated the developed procedure for plant protein expression by cloning two cassettes into the newly developed vectors and subsequently transferred them to Nicotiana benthamiana in a transient expression setup. Thereby we show that multigene constructs can be delivered into plant cells in a streamlined and highly efficient way. Our results will support faster introduction of synthetic biology into plant science. PMID:29300787

Convergent-Discriminant Validity of the Jewish Employment Vocational System (JEVS).

ERIC Educational Resources Information Center

Tryjankowski, Elaine M.

This study investigated the construct validity of five perceptual traits (auditory discrimination, visual discrimination, visual memory, visual-motor coordination, and auditory to visual-motor coordination) with five simulated work samples (union assembly, resistor reading, budgette assembly, lock assembly, and nail and screw sort) from the Jewish…

Development of concepts for low-cost energy storage assemblies for annual cycle energy system applications

NASA Astrophysics Data System (ADS)

Alexander, G. H.; Cooper, D. L.; Cummings, C. A.; Reiber, E. E.

1981-10-01

Low cost energy storage assemblies were developed. In the search for low overall cost assemblies, many diverse concepts and materials were postulated and briefly evaluated. Cost rankings, descriptions, and discussions of the concepts were presented from which ORNL selected the following three concepts for the Phase 2 development: (1) a site constructed tank with reinforced concrete walls formed with specialized modular blocks which eliminates most concrete form work and provides integral R-20 insulation designated ORNLFF; (2) a site constructed tank with earth supported walls that are formed from elements common to residential, in-ground swimming pools, designated SWPL; (3) and a site assembled tank used in underground utility vaults, designated UTLBX. Detailed designs of free standing versions of the three concepts are presented.

Building superlattices from individual nanoparticles via template-confined DNA-mediated assembly

NASA Astrophysics Data System (ADS)

Lin, Qing-Yuan; Mason, Jarad A.; Li, Zhongyang; Zhou, Wenjie; O’Brien, Matthew N.; Brown, Keith A.; Jones, Matthew R.; Butun, Serkan; Lee, Byeongdu; Dravid, Vinayak P.; Aydin, Koray; Mirkin, Chad A.

2018-02-01

DNA programmable assembly has been combined with top-down lithography to construct superlattices of discrete, reconfigurable nanoparticle architectures on a gold surface over large areas. Specifically, the assembly of individual colloidal plasmonic nanoparticles with different shapes and sizes is controlled by oligonucleotides containing “locked” nucleic acids and confined environments provided by polymer pores to yield oriented architectures that feature tunable arrangements and independently controllable distances at both nanometer- and micrometer-length scales. These structures, which would be difficult to construct by other common assembly methods, provide a platform to systematically study and control light-matter interactions in nanoparticle-based optical materials. The generality and potential of this approach are explored by identifying a broadband absorber with a solvent polarity response that allows dynamic tuning of visible light absorption.

Building superlattices from individual nanoparticles via template-confined DNA-mediated assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Qing-Yuan; Mason, Jarad A.; Li, Zhongyang

DNA programmable assembly has been combined with top-down lithography to construct superlattices of discrete, reconfigurable nanoparticle architectures on a gold surface over large areas. Specifically, individual colloidal plasmonic nanoparticles with different shapes and sizes are assembled with ‘locked” nucleic acids in polymer pores into oriented architectures that feature tunable arrangements and independently controllable distances at both nanometer and micrometer length scales. These structures, which would be difficult to construct via other common assembly methods, provide a platform to systematically study and control light-matter interactions in nanoparticle-based optical materials. The generality and potential of this approach is explored by identifying amore » broadband absorber with a solvent polarity response that allows dynamic tuning of the wavelength response and amplitude of visible light absorption.« less

Catalytic diversity in self-propagating peptide assemblies

NASA Astrophysics Data System (ADS)

Omosun, Tolulope O.; Hsieh, Ming-Chien; Childers, W. Seth; Das, Dibyendu; Mehta, Anil K.; Anthony, Neil R.; Pan, Ting; Grover, Martha A.; Berland, Keith M.; Lynn, David G.

2017-08-01

The protein-only infectious agents known as prions exist within cellular matrices as populations of assembled polypeptide phases ranging from particles to amyloid fibres. These phases appear to undergo Darwinian-like selection and propagation, yet remarkably little is known about their accessible chemical and biological functions. Here we construct simple peptides that assemble into well-defined amyloid phases and define paracrystalline surfaces able to catalyse specific enantioselective chemical reactions. Structural adjustments of individual amino acid residues predictably control both the assembled crystalline order and their accessible catalytic repertoire. Notably, the density and proximity of the extended arrays of enantioselective catalytic sites achieve template-directed polymerization of new polymers. These diverse amyloid templates can now be extended as dynamic self-propagating templates for the construction of even more complex functional materials.

Design of Bioinorganic Materials at the Interface of Coordination and Biosupramolecular Chemistry.

PubMed

Maity, Basudev; Ueno, Takafumi

2017-04-01

Protein assemblies have recently become known as potential molecular scaffolds for applications in materials science and bio-nanotechnology. Efforts to design protein assemblies for construction of protein-based hybrid materials with metal ions, metal complexes, nanomaterials and proteins now represent a growing field with a common aim of providing novel functions and mimicking natural functions. However, the important roles of protein assemblies in coordination and biosupramolecular chemistry have not been systematically investigated and characterized. In this personal account, we focus on our recent progress in rational design of protein assemblies using bioinorganic chemistry for (1) exploration of unnatural reactions, (2) construction of functional protein architectures, and (3) in vivo applications. © 2017 The Chemical Society of Japan & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Rotational joint assembly and method for constructing the same

NASA Technical Reports Server (NTRS)

Bandera, Pablo (Inventor); Buchele, Paul (Inventor)

2012-01-01

A rotational joint assembly and a method for constructing a rotational joint assembly are provided. The rotational joint assembly includes a first rotational component, a second rotational component coupled to the first rotational component such that the second rotational component is rotatable relative to the first rotational component in first and second rotational directions about an axis, and a flexure member, being deflectable in first and second deflection directions, coupled to at least one of the first and second rotational components such that when the second rotational component is rotated relative to the first rotational component in each of the first and second rotational directions about the axis, the flexure member is deflected in the first deflection direction and exerts a force on the second rotational component opposing the rotation.

Constructing Cost-Effective Crystal Structures with Table Tennis Balls and Tape That Allows Students to Assemble and Model Multiple Unit Cells

ERIC Educational Resources Information Center

Elsworth, Catherine; Li, Barbara T. Y.; Ten, Abilio

2017-01-01

In this letter we present an innovative and cost-effective method of constructing crystal structures using Dual Lock fastening adhesive tape with table tennis (ping pong) balls. The use of these fasteners allows the balls to be easily assembled into layers to model various crystal structures and unit cells and then completely disassembled again.…

[MATCHE: Management Approach to Teaching Consumer and Homemaking Education.] Occupational Strand: Textiles and Clothing. Module II-D-2: Assembly Line Garment Construction.

ERIC Educational Resources Information Center

Henry, Nina

This competency-based preservice home economics teacher education module on assembly line garment construction is the second in a set of three modules on occupational aspects of textiles and clothing. (This set is part of a larger series of sixty-seven modules on the Management Approach to Teaching Consumer and Homemaking Education [MATCHE]--see…

Process Development for Automated Solar Cell and Module Production. Task 4: Automated Array Assembly

NASA Technical Reports Server (NTRS)

Hagerty, J. J.

1981-01-01

The Automated Lamination Station is mechanically complete and is currently undergoing final wiring. The high current driver and isolator boards have been completed and installed, and the main interface board is under construction. The automated vacuum chamber has had a minor redesign to increase stiffness and improve the cover open/close mechanism. Design of the Final Assembly Station has been completed and construction is underway.

78 FR 46239 - Designations of Chief Acquisition Officer and Senior Procurement Executive

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-30

... Designations of Chief Acquisition Officer and Senior Procurement Executive and Delegation of Procurement... No. FR-5701-D-01] Designations of Chief Acquisition Officer and Senior Procurement Executive AGENCY... Procurement Officer as the Senior Procurement Executive. DATES: Effective Date: July 24, 2013. FOR FURTHER...

48 CFR 752.225-71 - Local procurement.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Local procurement. 752.225... Local procurement. For use in any USAID contract involving performance overseas. Local Procurement (FEB 1997) (a) Local procurement involves the use of appropriated funds to finance the procurement of goods...

48 CFR 752.225-71 - Local procurement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Local procurement. 752.225... Local procurement. For use in any USAID contract involving performance overseas. Local Procurement (FEB 1997) (a) Local procurement involves the use of appropriated funds to finance the procurement of goods...

The self-assembled behavior of DNA bases on the interface.

PubMed

Liu, Lei; Xia, Dan; Klausen, Lasse H; Dong, Mingdong

2014-01-27

A successful example of self-assembly in a biological system is that DNA can be an excellent agent to self-assemble into desirable two and three-dimensional nanostructures in a well-ordered manner by specific hydrogen bonding interactions between the DNA bases. The self-assembly of DNA bases have played a significant role in constructing the hierarchical nanostructures. In this review article we will introduce the study of nucleic acid base self-assembly by scanning tunneling microscopy (STM) at vacuum and ambient condition (the liquid/solid interface), respectively. From the ideal condition to a more realistic environment, the self-assembled behaviors of DNA bases are introduced. In a vacuum system, the energetic advantages will dominate the assembly formation of DNA bases, while at ambient condition, more factors such as conformational freedom and the biochemical environment will be considered. Therefore, the assemblies of DNA bases at ambient condition are different from the ones obtained under vacuum. We present the ordered nanostructures formed by DNA bases at both vacuum and ambient condition. To construct and tailor the nanostructure through the interaction between DNA bases, it is important to understand the assembly behavior and features of DNA bases and their derivatives at ambient condition. The utilization of STM offers the advantage of investigating DNA base self-assembly with sub-molecular level resolution at the surface.

The Self-Assembled Behavior of DNA Bases on the Interface

PubMed Central

Liu, Lei; Xia, Dan; Klausen, Lasse H.; Dong, Mingdong

2014-01-01

A successful example of self-assembly in a biological system is that DNA can be an excellent agent to self-assemble into desirable two and three-dimensional nanostructures in a well-ordered manner by specific hydrogen bonding interactions between the DNA bases. The self-assembly of DNA bases have played a significant role in constructing the hierarchical nanostructures. In this review article we will introduce the study of nucleic acid base self-assembly by scanning tunneling microscopy (STM) at vacuum and ambient condition (the liquid/solid interface), respectively. From the ideal condition to a more realistic environment, the self-assembled behaviors of DNA bases are introduced. In a vacuum system, the energetic advantages will dominate the assembly formation of DNA bases, while at ambient condition, more factors such as conformational freedom and the biochemical environment will be considered. Therefore, the assemblies of DNA bases at ambient condition are different from the ones obtained under vacuum. We present the ordered nanostructures formed by DNA bases at both vacuum and ambient condition. To construct and tailor the nanostructure through the interaction between DNA bases, it is important to understand the assembly behavior and features of DNA bases and their derivatives at ambient condition. The utilization of STM offers the advantage of investigating DNA base self-assembly with sub-molecular level resolution at the surface. PMID:24473140

Professional education and hospital development for organ donation.

PubMed

Fukushima, N; Konaka, S; Kato, O; Ashikari, J

2012-05-01

Because of the strict Organ Transplantation Act, only 81 brain dead (BD) organ donations had been performed in Japan for 13 years since 1997. The Act was revised on July 17, 2010, allowing, organs to be donated after BD with consent from the family, if the subject had not denied organ donation previously. This act has lead to an expectation of a 6-7-fold increase in BD donation. The 82 organ procurement coordinators (OPC) in Japan include 32 belonging to the Japanese Organ Network (JOT) and the others to each administrative division. JOT has guideline manuals of standard roles and procedures of OPC during organ procurement from BD and cardiac death donors. To manage the increased organ donations after the revision of the act, we have modified the education system. First, we modified the guideline manuals for OPC to correspond to the revised Transplant Act and governmental guidelines. Second, all OPC gathered in a meeting room to learn the new organ procurement system to deal with the revised Transplant Act and guidelines. Third, a special education program for 2 months was provided for the 10 newcomers. Last, the practical training in each donor case for newcomers was performed by older OPC. Topics of the education program were the revised transplant act and guidelines, family approach to organ donation, BD diagnosis, donor evaluation and management, organ procurement and preservation, allocation system, hospital development and family care. In the future, each OPC will be divided into special categories, such as the donor family OPC, the donor management OPC, and the operating room OPC. Therefore, we need to construct separate special education programs for each category. Copyright © 2012 Elsevier Inc. All rights reserved.

A near term space demonstration program for large structures

NASA Technical Reports Server (NTRS)

Nathan, C. A.

1978-01-01

For applications involving an employment of ultralarge structures in space, it would be necessary to have some form of space fabrication and assembly in connection with launch vehicle payload and volume limitations. The findings of a recently completed NASA sponsored study related to an orbital construction demonstration are reported. It is shown how a relatively small construction facility which is assembled in three shuttle flights can substantially advance space construction know-how and provide the nation with a permanent shuttle tended facility that can further advance large structures technologies and provide a construction capability for deployment of large structural systems envisioned for the late 1980s. The large structures applications identified are related to communications, navigation, earth observation, energy systems, radio astronomy, illumination, space colonization, and space construction.

Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation.

PubMed

Yamazaki, Tomohiro; Souquere, Sylvie; Chujo, Takeshi; Kobelke, Simon; Chong, Yee Seng; Fox, Archa H; Bond, Charles S; Nakagawa, Shinichi; Pierron, Gerard; Hirose, Tetsuro

2018-06-21

A class of long noncoding RNAs (lncRNAs) has architectural functions in nuclear body construction; however, specific RNA domains dictating their architectural functions remain uninvestigated. Here, we identified the domains of the architectural NEAT1 lncRNA that construct paraspeckles. Systematic deletion of NEAT1 portions using CRISPR/Cas9 in haploid cells revealed modular domains of NEAT1 important for RNA stability, isoform switching, and paraspeckle assembly. The middle domain, containing functionally redundant subdomains, was responsible for paraspeckle assembly. Artificial tethering of the NONO protein to a NEAT1_2 mutant lacking the functional subdomains rescued paraspeckle assembly, and this required the NOPS dimerization domain of NONO. Paraspeckles exhibit phase-separated properties including susceptibility to 1,6-hexanediol treatment. RNA fragments of the NEAT1_2 subdomains preferentially bound NONO/SFPQ, leading to phase-separated aggregates in vitro. Thus, we demonstrate that the enrichment of NONO dimers on the redundant NEAT1_2 subdomains initiates construction of phase-separated paraspeckles, providing mechanistic insights into lncRNA-based nuclear body formation. Copyright © 2018 Elsevier Inc. All rights reserved.

The Challenge of Access: Using Road Construction as a Tool in Counterinsurgency

DTIC Science & Technology

2011-06-10

northward, as shown in figure 9. Built by the United States in the 1950s, the dam and its downstream channels provide water for irrigation across the...12. DISTRIBUTION / AVAILABILITY STATEMENT Approved for Public Release; Distribution is Unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT This...consolidated Russian control. It also brought Russia closer to an overriding strategic objective, the procurement of a warm water port from which to project

Naval Procurement Problems: Theory and Practice

DTIC Science & Technology

1982-01-01

contrast when construction is contracted for the product may be modified before delivery and new information of relevance to both parties may be learned en...defense policy which may be desirable. For example, what price are we willing to pay for flexibility and surge capacity is a defense policy question...work force by an order of magnitude and achieve the productivity used as a basis for contract pricing was a primary factor in the schedule slippage

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2009-04-07

and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f...disrupting the optimal construction sequence and application of lessons learned for follow-on vessels in these programs...statements among others, although not necessarily in the order shown below: • “I am deeply concerned about Northrop Grumman Ship Systems’ ( NGSS

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2010-07-01

Background, Issues, and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK...performed out of sequence and significant rework has been required, disrupting the optimal construction sequence and application of lessons learned...deeply concerned about Northrop Grumman Ship Systems’ ( NGSS ) ability to recover in the aftermath of Hurricane Katrina, particularly in regard to

Navy LPD-17 Amphibious Ship Procurement: Background, Issues, and Options for Congress

DTIC Science & Technology

2010-03-29

and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f...performed out of sequence and significant rework has been required, disrupting the optimal construction sequence and application of lessons learned...deeply concerned about Northrop Grumman Ship Systems’ ( NGSS ) ability to recover in the aftermath of Hurricane Katrina, particularly in regard to

Technical change in US industry: A cross-industry analysis

NASA Technical Reports Server (NTRS)

Nelson, R. R. (Editor)

1981-01-01

The nature of the public policies which have influenced the pace and pattern of technical progress in a number of American industries is studied with the view of assessing the broad effects of these policies. The industries studied are agriculture, pharmaceuticals, semiconductors, computers, civil aircraft, automobiles and residential construction. The policies considered include research and development funding as well as government procurement, education, information dissemination, patent protection, licensing, regulations, and anti-trust policies.

NATO Phase Zero Contracting - A Proposed Strategic and Operational Planning Construct Within the NATO Framework for Defense Planning and Standardization

DTIC Science & Technology

2015-02-12

become better stewards of scarce resources, to eliminate potential waste, and to reduce abuse of taxpayer money due to poor management, operational...stewards of scarce resources, to eliminate potential waste, and to reduce abuse of taxpayer money due to poor management, operational redundancy and...Conference—Best Practices and Lessons Learned and Training and Educating for Acquisition, Procurement and Contracting in Defense Institutions

CONSTRUCTION OF NUCLEAR FUEL ELEMENTS

DOEpatents

Weems, S.J.

1963-09-24

>A rib arrangement and an end construction for nuclearfuel elements laid end to end in a coolant tube are described. The rib arrangement is such that each fuel element, when separated from other fuel elements, fits loosely in the coolant tube and so can easily be inserted or withdrawn from the tube. The end construction of the fuel elements is such that the fuel elements when assembled end to end are keyed against relative rotation, and the ribs of each fuel element cooperate with the ribs of the adjacent fuel elements to give the assembled fuel elements a tight fit with the coolant tube. (AEC)

The highly successful safe remediation of the Fernald waste pits undertaken under the privatization model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cherry, Mark; Lojek, Dave; Murphy, Con

2003-02-23

Remediation of eight waste pits at the Department of Energy (DOE) Fernald site, located northwest of Cincinnati, Ohio, involves excavating approximately one million tonnes in-situ of low-level waste which were placed in pits during Fernald's production era. This unique project, one of the largest in the history of CERCLA/Superfund, includes uranium and thorium contaminated waste, soils and sludges. These wet soils and sludges are thermally dried in a processing facility to meet Department of Transportation (DOT) transportation and disposal facility waste acceptance criteria, loaded into railcars and shipped to the Envirocare waste disposal facility at Clive, Utah. This project ismore » now approximately 60% complete with more than 415,000 tonnes (460,000 tons) of waste material safely shipped in 74 unit trains to Envirocare. Work is scheduled to be completed in early 2005. Success to date demonstrates that a major DOE site remediation project can be safely and successfully executed in partnership with private industry, utilizing proven commercial best practices, existing site labor resources and support of local stakeholders. In 1997 under the DOE's privatization initiative, Fluor Fernald, Inc. (Fluor Fernald) solicited the services of the remediation industry to design, engineer, procure, construct, own and operate a facility that would undertake the remediation of the waste pits. The resulting procurement was awarded to IT Corporation, currently Shaw Environmental and Infrastructure, Inc. (Shaw). The contractor was required to finance the procurement and construction of its facilities and infrastructure. The contract was performance-based and payment would be made on the successful loadout of the waste from the facility on a per-ton basis meeting the Envirocare waste acceptance criteria. This paper details the performance to date, the challenges encountered, and the seamless partnering between DOE, the Environmental Protection Agency (EPA), Fluor Fernald, Shaw, labor un ions, and the local community in creating and executing a successful project.« less

Center for Space Construction Third Annual Symposium

NASA Technical Reports Server (NTRS)

1991-01-01

Viewgraphs from presentations given at the symposium are presented. The topics covered include the following: orbital assembly, large space structures, space stations, expert systems, lunar regolith and structure mechanics, lunar shelter construction from lunar resources, telerobotic rovers, lunar construction equipment, lunar based equipment, and lunar construction.

Metal-directed design of supramolecular protein assemblies

PubMed Central

Bailey, Jake B.; Subramanian, Rohit H.; Churchfield, Lewis A.

2016-01-01

Owing to their central roles in cellular signaling, construction, and biochemistry, protein-protein interactions (PPIs) and protein self-assembly have become a major focus of molecular design and synthetic biology. In order to circumvent the complexity of constructing extensive non-covalent interfaces, which are typically involved in natural PPIs and protein self-assembly, we have developed two design strategies, Metal-Directed Protein Self-Assembly (MDPSA) and Metal-Templated Interface Redesign (MeTIR). These strategies, inspired by both the proposed evolutionary roles of metals and their prevalence in natural PPIs, take advantage of the favorable properties of metal coordination (bonding strength, directionality, and reversibility) to guide protein self-assembly with minimal design and engineering. Using a small, monomeric protein (cytochrome cb562) as a model building block, we employed MDPSA and MeTIR to create a diverse array of functional supramolecular architectures which range from structurally tunable oligomers to metalloprotein complexes that can properly self-assemble in living cells into novel metalloenzymes. The design principles and strategies outlined herein should be readily applicable to other protein systems with the goal of creating new PPIs and protein assemblies with structures and functions not yet produced by natural evolution. PMID:27586336

Cloning should be simple: Escherichia coli DH5α-mediated assembly of multiple DNA fragments with short end homologies

DOE PAGES

Kostylev, Maxim; Otwell, Anne E.; Richardson, Ruth E.; ...

2015-09-08

Numerous DNA assembly technologies exist for generating plasmids for biological studies. Many procedures require complex in vitro or in vivo assembly reactions followed by plasmid propagation in recombination-impaired Escherichia coli strains such as DH5α, which are optimal for stable amplification of the DNA materials. Here we show that despite its utility as a cloning strain, DH5α retains sufficient recombinase activity to assemble up to six doublestranded DNA fragments ranging in size from 150 bp to at least 7 kb into plasmids in vivo. This process also requires surprisingly small amounts of DNA, potentially obviating the need for upstream assembly processesmore » associated with most common applications of DNA assembly. In addition, we demonstrate the application of this process in cloning of various DNA fragments including synthetic genes, preparation of knockout constructs, and incorporation of guide RNA sequences in constructs for clustered regularly interspaced short palindromic repeats (CRISPR) genome editing. This consolidated process for assembly and amplification in a widely available strain of E. coli may enable productivity gain across disciplines involving recombinant DNA work.« less

Cloning should be simple: Escherichia coli DH5α-mediated assembly of multiple DNA fragments with short end homologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kostylev, Maxim; Otwell, Anne E.; Richardson, Ruth E.

Numerous DNA assembly technologies exist for generating plasmids for biological studies. Many procedures require complex in vitro or in vivo assembly reactions followed by plasmid propagation in recombination-impaired Escherichia coli strains such as DH5α, which are optimal for stable amplification of the DNA materials. Here we show that despite its utility as a cloning strain, DH5α retains sufficient recombinase activity to assemble up to six doublestranded DNA fragments ranging in size from 150 bp to at least 7 kb into plasmids in vivo. This process also requires surprisingly small amounts of DNA, potentially obviating the need for upstream assembly processesmore » associated with most common applications of DNA assembly. In addition, we demonstrate the application of this process in cloning of various DNA fragments including synthetic genes, preparation of knockout constructs, and incorporation of guide RNA sequences in constructs for clustered regularly interspaced short palindromic repeats (CRISPR) genome editing. This consolidated process for assembly and amplification in a widely available strain of E. coli may enable productivity gain across disciplines involving recombinant DNA work.« less

49. Neg. No. 7268C, ca. 1930, PhotographerUnknown, OVERALL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

49. Neg. No. 7268C, ca. 1930, Photographer-Unknown, OVERALL VIEW OF THE FORD MOTOR COMPANY ASSEMBLY PLANT FROM THE HENRY FORD BRIDGE, PRIOR TO CONSTRUCTION OF THE PRESSED STEEL BUILDING - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Self-assembly of proglycinin and hybrid proglycinin synthesized in vitro from cDNA

PubMed Central

Dickinson, Craig D.; Floener, Liliane A.; Lilley, Glenn G.; Nielsen, Niels C.

1987-01-01

An in vitro system was developed that results in the self-assembly of subunit precursors into complexes that resemble those found naturally in the endoplasmic reticulum. Subunits of glycinin, the predominant seed protein of soybeans, were synthesized from modified cDNAs using a combination of the SP6 transcription and the rabbit reticulocyte translation systems. Subunits produced from plasmid constructions that encoded either Gy4 or Gy5 gene products, but modified such that their signal sequences were absent, self-assembled into trimers equivalent in size to those precursors found in the endoplasmic reticulum. In contrast, proteins synthesized in vitro from Gy4 constructs failed to self-assemble when the signal sequence was left intact (e.g., preproglycinin) or when the coding sequence was modified to remove 27 amino acids from an internal hydrophobic region, which is highly conserved among the glycinin subunits. Various hybrid subunits were also produced by trading portions of Gy4 and Gy5 cDNAs and all self-assembled in our system. The in vitro assembly system provides an opportunity to study the self-assembly of precursors and to probe for regions important for assembly. It will also be helpful in attempts to engineer beneficial nutritional changes into this important food protein. Images PMID:16593868

Directions for lunar construction - A derivation of requirements from a construction scenario analysis

NASA Technical Reports Server (NTRS)

Dias, William S.; Matijevic, Jacob R.; Venkataraman, Subramani T.; Smith, Jeffrey H.; Lindemann, Randel A.; Levin, Richard R.

1992-01-01

This paper provides an initial trade-off study among several lunar construction options available to the Space Exploration Initiative. The relative time effectiveness of Extra-Vehicular Activity (EVA), Intra-Vehicular Activity (IVA), and Earth-based remote control assembly and construction methods are studied. Also considered is whether there is any construction time savings to building roads in advance, or surveying the construction sites with orbiters or rovers in advance. The study was conducted by adding detail to a potentially real scenario - a nuclear power plant - and applying time multipliers for the various control options and terrain alternatives, provided by roboticists among the authors. The authors conclude that IVA is a faster construction method than either EVA or construction conducted remotely from Earth. Surveying proposed sites in advance, with orbiters and rovers, provides a significant time savings through adding to certainty, and therefore may be cost effective. Developing a heavy-lift launch capability and minimizing assembly and construction processes by landing large payloads is probably worthwhile to the degree possible, as construction activities would use a large amount of surface operations time.

Transformation of fraud activities in procurement system in Indonesia

NASA Astrophysics Data System (ADS)

Setiani, N.; Huda, S. N.; Pulungan, R.; Winarko, E.

2017-03-01

E-procurement has been applied in Indonesia since 2008 through the adoption of Electronic Procurement System (SPSE). The purpose of the use of SPSE in the procurement process is to improve the efficiency of goods or services procurement. In the bidding process, intensive communication and data exchange between providers and organizers are urgently needed. Through SPSE, the frequency of face to face meetings between providers and the committee can be reduced. This is expected to minimize potential fraud behaviors in the goods or services procurement conducted through the tender process. There exists a transformation of activities in the procurement through SPSE from the tender process that was previously done manually. In this paper, we analyze this transformation between manual procurement process and SPSE-based procurement process. The result of the analysis is exploited for investigating the possibility of fraud behavior transformations in every phase of e-procurement activities.

Spectral and Wavefront Error Performance of WFIRST/AFTA Prototype Filters

NASA Technical Reports Server (NTRS)

Quijada, Manuel; Seide, Laurie; Marx, Cathy; Pasquale, Bert; McMann, Joseph; Hagopian, John; Dominguez, Margaret; Gong, Qian; Morey, Peter

2016-01-01

The Cycle 5 design baseline for the Wide-Field Infrared Survey Telescope Astrophysics Focused Telescope Assets (WFIRSTAFTA) instrument includes a single wide-field channel (WFC) instrument for both imaging and slit-less spectroscopy. The only routinely moving part during scientific observations for this wide-field channel is the element wheel (EW) assembly. This filter-wheel assembly will have 8 positions that will be populated with 6 bandpass filters, a blank position, and a Grism that will consist of a three-element assembly to disperse the full field with an undeviated central wavelength for galaxy redshift surveys. All filter elements in the EW assembly will be made out of fused silica substrates (110 mm diameter) that will have the appropriate bandpass coatings according to the filter designations (Z087, Y106, J129, H158, F184, W149 and Grism). This paper presents and discusses the performance (including spectral transmission and reflectedtransmitted wavefront error measurements) of a subset of bandpass filter coating prototypes that are based on the WFC instrument filter compliment. The bandpass coating prototypes that are tested in this effort correspond to the Z087, W149, and Grism filter elements. These filter coatings have been procured from three different vendors to assess the most challenging aspects in terms of the in-band throughput, out of band rejection (including the cut-on and cutoff slopes), and the impact the wavefront error distortions of these filter coatings will have on the imaging performance of the de-field channel in the WFIRSTAFTA observatory.

Operating a petabyte class archive at ESO

NASA Astrophysics Data System (ADS)

Suchar, Dieter; Lockhart, John S.; Burrows, Andrew

2008-07-01

The challenges of setting up and operating a Petabyte Class Archive will be described in terms of computer systems within a complex Data Centre environment. The computer systems, including the ESO Primary and Secondary Archive and the associated computational environments such as relational databases will be explained. This encompasses the entire system project cycle, including the technical specifications, procurement process, equipment installation and all further operational phases. The ESO Data Centre construction and the complexity of managing the environment will be presented. Many factors had to be considered during the construction phase, such as power consumption, targeted cooling and the accumulated load on the building structure to enable the smooth running of a Petabyte class Archive.

The Danish Organic Action Plan 2020: assessment method and baseline status of organic procurement in public kitchens.

PubMed

Sørensen, Nina N; Lassen, Anne D; Løje, Hanne; Tetens, Inge

2015-09-01

With political support from the Danish Organic Action Plan 2020, organic public procurement in Denmark is expected to increase. In order to evaluate changes in organic food procurement in Danish public kitchens, reliable methods are needed. The present study aimed to compare organic food procurement measurements by two methods and to collect and discuss baseline organic food procurement measurements from public kitchens participating in the Danish Organic Action Plan 2020. Comparison study measuring organic food procurement by applying two different methods, one based on the use of procurement invoices (the Organic Cuisine Label method) and the other on self-reported procurement (the Dogme method). Baseline organic food procurement status was based on organic food procurement measurements and background information from public kitchens. Public kitchens participating in the six organic food conversion projects funded by the Danish Organic Action Plan 2020 during 2012 and 2013. Twenty-six public kitchens (comparison study) and 345 public kitchens (baseline organic food procurement status). A high significant correlation coefficient was found between the two organic food procurement measurement methods (r=0·83, P<0·001) with measurements relevant for the baseline status. Mean baseline organic food procurement was found to be 24 % when including measurements from both methods. The results indicate that organic food procurement measurements by both methods were valid for the baseline status report of the Danish Organic Action Plan 2020. Baseline results in Danish public kitchens suggest there is room for more organic as well as sustainable public procurement in Denmark.

The trajectories of Prevention through Design in construction.

PubMed

Toole, T Michael; Gambatese, John

2008-01-01

Construction Hazards Prevention through Design (CHPtD) is a process in which engineers and architects explicitly consider the safety of construction workers during the design process. Although articles on CHPtD have appeared in top construction journals, the literature has not addressed technical principles underlying CHPtD to help designers better perform CHPtD, to facilitate the development of additional CHPtD tools, and to predict the future path of CHPtD. This theoretical paper uses the existing literature on CHPtD and current action research associated with several CHPtD workgroups to analyze how CHPtD will likely evolve over the coming decades. There are four trajectories along which CHPtD will progress. (a) Designs will increasingly facilitate prefabricated construction; (b) designers will increasingly choose materials and systems that are inherently safer than alternatives; (c) designers will increasingly perform construction engineering; and (d) designers will increasingly apply spatial considerations to reduce worker hazards. By understanding how CHPtD may be manifested in the engineering-procurement-construction (EPC) industry, practitioners can better prepare for adopting CHPtD within their organizations and construction and engineering educators can better prepare their graduates to perform CHPtD.

Construction concept for erecting an offset-fed antenna

NASA Technical Reports Server (NTRS)

Rhodes, M. D.

1984-01-01

A design concept for the construction of an offset-fed antenna is discussed. Antennas of this type are of interest for space applications because the configuration eliminates the effects of feed and feed-support blockage. The proposed construction concept is developed around the assembly of a stiff truss substructure by pressure-suited astronauts operating in extravehicular activity (EVA) assisted by a mobile platform that moves along the structure to position the astronauts at joint locations where they can latch members in place. Construction can be accomplished from the shuttle cargo bay in the course of a normal flight or from a space station platform. The concepts demonstrates the versatility of machine assisted manned assembly and is only one of many potential applications.

Construction concepts and validation of the 3D printed UST_2 modular stellarator

NASA Astrophysics Data System (ADS)

Queral, V.

2015-03-01

High accuracy, geometric complexity and thus high cost of stellarators tend to hinder the advance of stellarator research. Nowadays, new manufacturing methods might be developed for the production of small and middle-size stellarators. The methods should demonstrate advantages with respect common fabrication methods, like casting, cutting, forging and welding, for the construction of advanced highly convoluted modular stellarators. UST2 is a small modular three period quasi-isodynamic stellarator of major radius 0.26 m and plasma volume 10 litres being currently built to validate additive manufacturing (3D printing) for stellarator construction. The modular coils are wound in grooves defined on six 3D printed half period frames designed as light truss structures filled by a strong filler. A geometrically simple assembling configuration has been concocted for UST2 so as to try to lower the cost of the device while keeping the positioning accuracy of the different elements. The paper summarizes the construction and assembling concepts developed, the devised positioning methodology, the design of the coil frames and positioning elements and, an initial validation of the assembling of the components.

Orbital construction support equipment

NASA Technical Reports Server (NTRS)

1977-01-01

Approximately 200 separate construction steps were defined for the three solar power satellite (SPS) concepts. Detailed construction scenarios were developed which describe the specific tasks to be accomplished, and identify general equipment requirements. The scenarios were used to perform a functional analysis, which resulted in the definition of 100 distinct SPS elements. These elements are the components, parts, subsystems, or assemblies upon which construction activities take place. The major SPS elements for each configuration are shown. For those elements, 300 functional requirements were identified in seven generic processes. Cumulatively, these processes encompass all functions required during SPS construction/assembly. Individually each process is defined such that it includes a specific type of activity. Each SPS element may involve activities relating to any or all of the generic processes. The processes are listed, and examples of the requirements defined for a typical element are given.

Fluidic self-actuating control assembly

DOEpatents

Grantz, Alan L.

1979-01-01

A fluidic self-actuating control assembly for use in a reactor wherein no external control inputs are required to actuate (scram) the system. The assembly is constructed to scram upon sensing either a sudden depressurization of reactor inlet flow or a sudden increase in core neutron flux. A fluidic control system senses abnormal flow or neutron flux transients and actuates the system, whereupon assembly coolant flow reverses, forcing absorber balls into the reactor core region.

A (13)C NMR analysis of the effects of electron radiation on graphite/polyetherimide composites

NASA Technical Reports Server (NTRS)

Ferguson, Milton W.

1989-01-01

Initial investigations have been made into the use of high resolution nuclear magnetic resonance (NMR) for the characterization of radiation effects in graphite and Kevlar fibers, polymers, and the fiber/matrix interface in graphite/polyetherimide composites. Sample preparation techniques were refined. Essential equipment has been procured. A new NMR probe was constructed to increase the proton signal-to-noise ratio. Problem areas have been identified and plans developed to resolve them.

Creating Cost Growth Models for the Engineering and Manufacturing Development Phase of Acquisition Using Logistic and Multiple Regression

DTIC Science & Technology

2004-03-01

constant variance via an analysis of the residuals, as well as the Breusch - Pagan test (see Figure 3 below). As a result, we follow the footsteps of...reasonably normal, which ensures that our residuals meet the assumption of constant variance by passing the Breusch - Pagan test (see Figure 4 below...sections for Research and Development, Test and Evaluation (RDT&E), procurement and military construction (Jarvaise, 1996:3). While differing

Navy DDG-1000 and DDG-51 Destroyer Programs: Background, Oversight Issues, and Options for Congress

DTIC Science & Technology

2009-06-04

Aegis combat system and the arming of the ship with the SM-3, a version of the Navy’s Standard Missile that is designed for BMD operations.6 Total...and assigning the construction of those ships to GD/BIW and/or NGSB; • procuring AGS- armed versions of the basic LPD-17 class hull—another option...subcommittee of the House Armed Services Committee.24 22 Christopher P. Cavas, “All

Construction and Testing of an 80C86 Based Communications Controller for the Petite Amateur Navy Satellite (PANSAT)

DTIC Science & Technology

1990-12-01

S) Naval Postgraduate School 6a. NAME OF PERFORMING ORGANIZATION 6b OFFICE SYMBOL 7a NAME OF MONITORING ORGANIZATION (if applicable ) Code 33 6c...FUNDING/SPONSORING Bb. OFFICE SYMBOL 9. PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION (If applicable ) 8c. ADDRESS (City, State, and ZIP Code...system’s individual components. Then one derives the overall system reliability from that information, using a simple mathematical model, to be

40 CFR 35.6565 - Procurement methods.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Procurement methods. 35.6565 Section 35... Actions Procurement Requirements Under A Cooperative Agreement § 35.6565 Procurement methods. The... purchase procedures are those relatively simple and informal procurement methods for securing services...

Analysis of procurement processes and development of recommendations for intelligent transportation systems (ITS) procurements : final report.

DOT National Transportation Integrated Search

2007-09-01

Traditional state procurement processes are not well-suited to the procurement of Intelligent Transportation Systems (ITS). The objective of this study was to analyze Kentuckys existing procurement processes, identify strengths and weaknesses of e...

40 CFR 35.6565 - Procurement methods.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Procurement methods. 35.6565 Section 35... Actions Procurement Requirements Under A Cooperative Agreement § 35.6565 Procurement methods. The... purchase procedures are those relatively simple and informal procurement methods for securing services...

40 CFR 35.6565 - Procurement methods.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Procurement methods. 35.6565 Section 35... Actions Procurement Requirements Under A Cooperative Agreement § 35.6565 Procurement methods. The... purchase procedures are those relatively simple and informal procurement methods for securing services...

40 CFR 35.6565 - Procurement methods.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Procurement methods. 35.6565 Section 35... Actions Procurement Requirements Under A Cooperative Agreement § 35.6565 Procurement methods. The... purchase procedures are those relatively simple and informal procurement methods for securing services...

40 CFR 35.6565 - Procurement methods.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Procurement methods. 35.6565 Section 35... Actions Procurement Requirements Under A Cooperative Agreement § 35.6565 Procurement methods. The... purchase procedures are those relatively simple and informal procurement methods for securing services...

23 CFR 172.5 - Methods of procurement.

Code of Federal Regulations, 2012 CFR

2012-04-01

... ENGINEERING AND DESIGN RELATED SERVICE CONTRACTS § 172.5 Methods of procurement. (a) Procurement. The procurement of Federal-aid highway contracts for engineering and design related services shall be evaluated... negotiation. Contracting agencies shall use competitive negotiation for the procurement of engineering and...

22 CFR 201.23 - Procurement under private sector procedures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Procurement under private sector procedures... § 201.23 Procurement under private sector procedures. (a) General requirements. Procurements under private sector procedures will normally be carried out by importers using negotiated procurement...

DNA-templated assembly of viral protein hydrogel

NASA Astrophysics Data System (ADS)

Xu, Xin; Tao, Ailin; Xu, Yun

2014-11-01

Hydrogels are a promising class of biomaterials that can be easily tailored to produce a native extracellular matrix that exhibits desirable mechanical and chemical properties. Here we report the construction of a hydrogel via the assembly of cucumber mosaic virus (CMV) capsid protein and Y-shaped and cross-shaped DNAs.Hydrogels are a promising class of biomaterials that can be easily tailored to produce a native extracellular matrix that exhibits desirable mechanical and chemical properties. Here we report the construction of a hydrogel via the assembly of cucumber mosaic virus (CMV) capsid protein and Y-shaped and cross-shaped DNAs. Electronic supplementary information (ESI) available. See DOI: 10.1039/c4nr02414a

Research Advances on Fabricated Shear Wall System

NASA Astrophysics Data System (ADS)

Liu, Xudong; Wang, Donghui; Wang, Sheng; Zhai, Yu

2018-03-01

With the rapid development of the construction industry, building energy consumption has been increasing, has become a problem that can not be ignored. It is imperative to develop energy-saving buildings. A new type of prefabricated shear wall is assembled and partially assembled by prefabricated parts, and some concrete is spliced together. The new structure has good integrity, seismic resistance and excellent energy saving and environmental protection performance. It reduces building energy consumption to a great extent. Therefore, the design method, manufacturing process, site assembly process and key technical problems of the system are discussed. For the construction industry gradually entered the energy conservation, environmental protection, safety and durability of sustainable development laid the foundation.

Deconstructing Building Blocks: Preschoolers' Spatial Assembly Performance Relates to Early Mathematical Skills

ERIC Educational Resources Information Center

Verdine, Brian N.; Golinkoff, Roberta M.; Hirsh-Pasek, Kathryn; Newcombe, Nora S.; Filipowicz, Andrew T.; Chang, Alicia

2014-01-01

This study focuses on three main goals: First, 3-year-olds' spatial assembly skills are probed using interlocking block constructions (N = 102). A detailed scoring scheme provides insight into early spatial processing and offers information beyond a basic accuracy score. Second, the relation of spatial assembly to early mathematical skills…

Fire-resistant materials for aircraft passenger seat construction

NASA Technical Reports Server (NTRS)

Fewell, L. L.; Tesoro, G. C.; Moussa, A.; Kourtides, D. A.

1979-01-01

The thermal response characteristics of fabric and fabric-foam assemblies are described. The various aspects of the ignition behavior of contemporary aircraft passenger seat upholstery fabric materials relative to fabric materials made from thermally stable polymers are evaluated. The role of the polymeric foam backing on the thermal response of the fabric-foam assembly is also ascertained. The optimum utilization of improved fire-resistant fabric and foam materials in the construction of aircraft passenger seats is suggested.

75 FR 13262 - Procurement List Additions and Deletions

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

...: Additions to and deletions from the Procurement List. SUMMARY: This action adds to the Procurement List a... severe disabilities, and deletes from the Procurement List services previously furnished by such agencies... Severely Disabled published notices of proposed additions to the Procurement List. After consideration of...