Preface: ISBB Special Issue

USDA-ARS?s Scientific Manuscript database

This special issue is published for the International Society of Biocatalysis and Biotechnology (ISBB). The ISBB special issue is devoted to all areas of biocatalysis and agricultural biotechnology in which biological systems are developed and/or used for the provision of commercial goods or serv...

Preface: Biocatalysis and Agricultural Biotechnology

USDA-ARS?s Scientific Manuscript database

This book was assembled with the intent of bringing together current advances and in-depth reviews of biocatalysis and agricultural biotechnology with emphasis on bio-based products and agricultural biotechnology. Recent energy and food crises point out the importance of bio-based products from ren...

Lipase-Mediated Kinetic Resolution: An Introductory Approach to Practical Biocatalysis

ERIC Educational Resources Information Center

Bandeira, Pamela T.; Thomas, Juliana C.; de Oliveira, Alfredo R. M.; Piovan, Leandro

2017-01-01

An experimental protocol that provides an excellent way to discuss concepts at the crossroads of organic chemistry and biochemistry employing biocatalysis is presented. By evaluating several reaction parameters (enzyme source, organic solvent, and acyl donor), it was possible to conduct an enzymatic kinetic resolution experiment using…

Using Biocatalysis to Integrate Organic Chemistry into a Molecular Biology Laboratory Course

ERIC Educational Resources Information Center

Beers, Mande; Archer, Crystal; Feske, Brent D.; Mateer, Scott C.

2012-01-01

Current cutting-edge biomedical investigation requires that the researcher have an operational understanding of several diverse disciplines. Biocatalysis is a field of science that operates at the crossroads of organic chemistry, biochemistry, microbiology, and molecular biology, and provides an excellent model for interdisciplinary research. We…

Role of Biocatalysis in Sustainable Chemistry.

PubMed

Sheldon, Roger A; Woodley, John M

2018-01-24

Based on the principles and metrics of green chemistry and sustainable development, biocatalysis is both a green and sustainable technology. This is largely a result of the spectacular advances in molecular biology and biotechnology achieved in the past two decades. Protein engineering has enabled the optimization of existing enzymes and the invention of entirely new biocatalytic reactions that were previously unknown in Nature. It is now eminently feasible to develop enzymatic transformations to fit predefined parameters, resulting in processes that are truly sustainable by design. This approach has successfully been applied, for example, in the industrial synthesis of active pharmaceutical ingredients. In addition to the use of protein engineering, other aspects of biocatalysis engineering, such as substrate, medium, and reactor engineering, can be utilized to improve the efficiency and cost-effectiveness and, hence, the sustainability of biocatalytic reactions. Furthermore, immobilization of an enzyme can improve its stability and enable its reuse multiple times, resulting in better performance and commercial viability. Consequently, biocatalysis is being widely applied in the production of pharmaceuticals and some commodity chemicals. Moreover, its broader application will be further stimulated in the future by the emerging biobased economy.

Introducing the Concept of Biocatalysis in the Classroom: The Conversion of Cholesterol to Provitamin D[subscript 3

ERIC Educational Resources Information Center

De Luca, Belén M.; Nudel, Clara B.; Gonzalez, Rodrigo H.; Nusblat, Alejandro D.

2017-01-01

Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course "design and optimization of biological systems" which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for…

Enzymatic Inverse Opal Hydrogel Particles for Biocatalyst.

PubMed

Wang, Huan; Gu, Hongcheng; Chen, Zhuoyue; Shang, Luoran; Zhao, Ze; Gu, Zhongze; Zhao, Yuanjin

2017-04-19

Enzymatic carriers have a demonstrated value for chemical reactions and industrial applications. Here, we present a novel kind of inverse opal hydrogel particles as the enzymatic carriers. The particles were negatively replicated from spherical colloidal crystal templates by using magnetic nanoparticles tagged acrylamide hydrogel. Thus, they were endowed with the features of monodispersity, small volume, complete penetrating structure, and controllable motion, which are all beneficial for improving the efficiency of biocatalysis. In addition, due to the ordered porous nanostructure, the inverse opal hydrogel particles were imparted with unique photonic band gaps (PBGs) and vivid structural colors for encoding varieties of immobilized enzymes and for constructing a multienzymes biocatalysis system. These features of the inverse opal hydrogel particles indicate that they are ideal enzymatic carriers for biocatalysis.

Biocatalysis and biomimetics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Burrington, J.D.; Clark, D.S.

1989-01-01

The proceedings are divided into three parts: Bioscience and biotechnology; Structure-function relationships; and Biomimetics. Topics include: the chemistry of biotechnology, biomimetics, and biocatalysts; crystallography and mutagenesis; computerized simulation of biocatalysis and biomimetic processes; enzymatic reactions in micellar systems; hydroxylation of hydrocarbons; oxidation of lignin; zeolite catalysts as enzyme mimics; and immobilization of proteins and enzymes. Some papers have been processed separately for inclusion on the data base.

Redesigning of Microbial Cell Surface and Its Application to Whole-Cell Biocatalysis and Biosensors.

PubMed

Han, Lei; Zhao, Yukun; Cui, Shan; Liang, Bo

2018-06-01

Microbial cell surface display technology can redesign cell surfaces with functional proteins and peptides to endow cells some unique features. Foreign peptides or proteins are transported out of cells and immobilized on cell surface by fusing with anchoring proteins, which is an effective solution to avoid substance transfer limitation, enzyme purification, and enzyme instability. As the most frequently used prokaryotic and eukaryotic protein surface display system, bacterial and yeast surface display systems have been widely applied in vaccine, biocatalysis, biosensor, bioadsorption, and polypeptide library screening. In this review of bacterial and yeast surface display systems, different cell surface display mechanisms and their applications in biocatalysis as well as biosensors are described with their strengths and shortcomings. In addition to single enzyme display systems, multi-enzyme co-display systems are presented here. Finally, future developments based on our and other previous reports are discussed.

Industrial applications of enzyme biocatalysis: Current status and future aspects.

PubMed

Choi, Jung-Min; Han, Sang-Soo; Kim, Hak-Sung

2015-11-15

Enzymes are the most proficient catalysts, offering much more competitive processes compared to chemical catalysts. The number of industrial applications for enzymes has exploded in recent years, mainly owing to advances in protein engineering technology and environmental and economic necessities. Herein, we review recent progress in enzyme biocatalysis, and discuss the trends and strategies that are leading to broader industrial enzyme applications. The challenges and opportunities in developing biocatalytic processes are also discussed. Copyright © 2015 Elsevier Inc. All rights reserved.

Engineering Ultrastable Protein Filaments into 2D and 3D Templates for Advanced Nanomaterials: A New Dimension in Materials Design

DTIC Science & Technology

2013-08-16

Littlechild,   Thermophilic  archaeal   enzymes  and  applications  in  biocatalysis,  Biochem  Soc  Trans  39   (2011...spatially arrange thermostable enzymes for high- temperature biocatalysis[7][8] and as biotemplates for fabrication and patterning of inorganic...upon which to position hyperthermostable enzymes that have optimal activities at or above 100°C  [8], which are temperatures beyond the stability of

ECUT: Energy Conversion and utilization Technologies program biocatalysis research activity. Generation of chemical intermediates by catalytic oxidative decarboxylation of dilute organic acids

NASA Technical Reports Server (NTRS)

Distefano, S.; Gupta, A.; Ingham, J. D.

1983-01-01

A rhodium-based catalyst was prepared and preliminary experiments were completed where the catalyst appeared to decarboxylate dilute acids at concentrations of 1 to 10 vol%. Electron spin resonance spectroscoy was used to characterize the catalyst as a first step leading toward modeling and optimization of rhodium catalysts. Also, a hybrid chemical/biological process for the production of hydrocarbons has been assessed. These types of catalysts could greatly increase energy efficiency of this process.

Biocatalysis in the Pharmaceutical Industry: The Need for Speed

PubMed Central

2017-01-01

The use of biocatalysis in the pharmaceutical industry continues to expand as a result of increased access to enzymes and the ability to engineer those enzymes to meet the demands of industrial processes. However, we are still just scratching the surface of potential biocatalytic applications. The time pressures present in pharmaceutical process development are incompatible with the long lead times required for engineering a suitable biocatalyst. Dramatic increases in the speed of protein engineering are needed to deliver on the ever increasing opportunities for industrial biocatalytic processes. PMID:28523096

Biocatalysis in the Pharmaceutical Industry: The Need for Speed.

PubMed

Truppo, Matthew D

2017-05-11

The use of biocatalysis in the pharmaceutical industry continues to expand as a result of increased access to enzymes and the ability to engineer those enzymes to meet the demands of industrial processes. However, we are still just scratching the surface of potential biocatalytic applications. The time pressures present in pharmaceutical process development are incompatible with the long lead times required for engineering a suitable biocatalyst. Dramatic increases in the speed of protein engineering are needed to deliver on the ever increasing opportunities for industrial biocatalytic processes.

The University of Minnesota Biocatalysis/Biodegradation Database: specialized metabolism for functional genomics.

PubMed Central

Ellis, L B; Hershberger, C D; Wackett, L P

1999-01-01

The University of Minnesota Biocatalysis/Biodegradation Database (UM-BBD, http://www.labmed.umn.edu/umbbd/i nde x.html) first became available on the web in 1995 to provide information on microbial biocatalytic reactions of, and biodegradation pathways for, organic chemical compounds, especially those produced by man. Its goal is to become a representative database of biodegradation, spanning the diversity of known microbial metabolic routes, organic functional groups, and environmental conditions under which biodegradation occurs. The database can be used to enhance understanding of basic biochemistry, biocatalysis leading to speciality chemical manufacture, and biodegradation of environmental pollutants. It is also a resource for functional genomics, since it contains information on enzymes and genes involved in specialized metabolism not found in intermediary metabolism databases, and thus can assist in assigning functions to genes homologous to such less common genes. With information on >400 reactions and compounds, it is poised to become a resource for prediction of microbial biodegradation pathways for compounds it does not contain, a process complementary to predicting the functions of new classes of microbial genes. PMID:9847233

Application of combinatorial biocatalysis for a unique ring expansion of dihydroxymethylzearalenone.

PubMed

Rich, Joseph O; Budde, Cheryl L; McConeghey, Luke D; Cotterill, Ian C; Mozhaev, Vadim V; Singh, Sheo B; Goetz, Michael A; Zhao, Annie; Michels, Peter C; Khmelnitsky, Yuri L

2009-06-01

Combinatorial biocatalysis was applied to generate a diverse set of dihydroxymethylzearalenone analogs with modified ring structure. In one representative chemoenzymatic reaction sequence, dihydroxymethylzearalenone was first subjected to a unique enzyme-catalyzed oxidative ring opening reaction that creates two new carboxylic groups on the molecule. These groups served as reaction sites for further derivatization involving biocatalytic ring closure reactions with structurally diverse bifunctional reagents, including different diols and diamines. As a result, a library of cyclic bislactones and bislactams was created, with modified ring structures covering chemical space and structure activity relationships unattainable by conventional synthetic means.

Radiofrequency treatment enhances the catalytic function of an immobilized nanobiohybrid catalyst

NASA Astrophysics Data System (ADS)

San, Boi Hoa; Ha, Eun-Ju; Paik, Hyun-Jong; Kim, Kyeong Kyu

2014-05-01

Biocatalysis, the use of enzymes in chemical transformation, has undergone intensive development for a wide range of applications. As such, maximizing the functionality of enzymes for biocatalysis is a major priority to enable industrial use. To date, many innovative technologies have been developed to address the future demand of enzymes for these purposes, but maximizing the catalytic activity of enzymes remains a challenge. In this study, we demonstrated that the functionality of a nanobiocatalyst could be enhanced by combining immobilization and radiofrequency (RF) treatment. Aminopeptidase PepA-encapsulating 2 nm platinum nanoparticles (PepA-PtNPs) with the catalytic activities of hydrolysis and hydrogenation were employed as multifunctional nanobiocatalysts. Immobilizing the nanobiocatalysts in a hydrogel using metal chelation significantly enhanced their functionalities, including catalytic power, thermal-stability, pH tolerance, organic solvent tolerance, and reusability. Most importantly, RF treatment of the hydrogel-immobilized PepA-PtNPs increased their catalytic power by 2.5 fold greater than the immobilized PepA. Our findings indicate that the catalytic activities and functionalities of PepA-PtNPs are greatly enhanced by the combination of hydrogel-immobilization and RF treatment. Based on our findings, we propose that RF treatment of nanobiohybrid catalysts immobilized on the bulk hydrogel represents a new strategy for achieving efficient biocatalysis.Biocatalysis, the use of enzymes in chemical transformation, has undergone intensive development for a wide range of applications. As such, maximizing the functionality of enzymes for biocatalysis is a major priority to enable industrial use. To date, many innovative technologies have been developed to address the future demand of enzymes for these purposes, but maximizing the catalytic activity of enzymes remains a challenge. In this study, we demonstrated that the functionality of a nanobiocatalyst could be enhanced by combining immobilization and radiofrequency (RF) treatment. Aminopeptidase PepA-encapsulating 2 nm platinum nanoparticles (PepA-PtNPs) with the catalytic activities of hydrolysis and hydrogenation were employed as multifunctional nanobiocatalysts. Immobilizing the nanobiocatalysts in a hydrogel using metal chelation significantly enhanced their functionalities, including catalytic power, thermal-stability, pH tolerance, organic solvent tolerance, and reusability. Most importantly, RF treatment of the hydrogel-immobilized PepA-PtNPs increased their catalytic power by 2.5 fold greater than the immobilized PepA. Our findings indicate that the catalytic activities and functionalities of PepA-PtNPs are greatly enhanced by the combination of hydrogel-immobilization and RF treatment. Based on our findings, we propose that RF treatment of nanobiohybrid catalysts immobilized on the bulk hydrogel represents a new strategy for achieving efficient biocatalysis. Electronic supplementary information (ESI) available. See DOI: 10.1039/c4nr00407h

Magnetic MOF microreactors for recyclable size-selective biocatalysis† †Electronic supplementary information (ESI) available: Experimental procedures, calibration curves and additional figures relating to capsule characterisation and biocatalysis. See DOI: 10.1039/c4sc03367a Click here for additional data file.

PubMed Central

Huo, Jia; Aguilera-Sigalat, Jordi; El-Hankari, Samir

2015-01-01

In this contribution we report a synthetic strategy for the encapsulation of functional biomolecules within MOF-based microcapsules. We employ an agarose hydrogel droplet Pickering-stabilised by UiO-66 and magnetite nanoparticles as a template around which to deposit a hierarchically structured ZIF-8 shell. The resulting microcapsules are robust, highly microporous and readily attracted to a magnet, where the hydrogel core provides a facile means to encapsulate enzymes for recyclable size-selective biocatalysis. PMID:28717454

The role of electrostatic interactions in protease surface diffusion and the consequence for interfacial biocatalysis.

PubMed

Feller, Bob E; Kellis, James T; Cascão-Pereira, Luis G; Robertson, Channing R; Frank, Curtis W

2010-12-21

This study examines the influence of electrostatic interactions on enzyme surface diffusion and the contribution of diffusion to interfacial biocatalysis. Surface diffusion, adsorption, and reaction were investigated on an immobilized bovine serum albumin (BSA) multilayer substrate over a range of solution ionic strength values. Interfacial charge of the enzyme and substrate surface was maintained by performing the measurements at a fixed pH; therefore, electrostatic interactions were manipulated by changing the ionic strength. The interfacial processes were investigated using a combination of techniques: fluorescence recovery after photobleaching, surface plasmon resonance, and surface plasmon fluorescence spectroscopy. We used an enzyme charge ladder with a net charge ranging from -2 to +4 with respect to the parent to systematically probe the contribution of electrostatics in interfacial enzyme biocatalysis on a charged substrate. The correlation between reaction rate and adsorption was determined for each charge variant within the ladder, each of which displayed a maximum rate at an intermediate surface concentration. Both the maximum reaction rate and adsorption value at which this maximum rate occurs increased in magnitude for the more positive variants. In addition, the specific enzyme activity increased as the level of adsorption decreased, and for the lowest adsorption values, the specific enzyme activity was enhanced compared to the trend at higher surface concentrations. At a fixed level of adsorption, the specific enzyme activity increased with positive enzyme charge; however, this effect offers diminishing returns as the enzyme becomes more highly charged. We examined the effect of electrostatic interactions on surface diffusion. As the binding affinity was reduced by increasing the solution ionic strength, thus weakening electrostatic interaction, the rate of surface diffusion increased considerably. The enhancement in specific activity achieved at the lowest adsorption values is explained by the substantial rise in surface diffusion at high ionic strength due to decreased interactions with the surface. Overall, knowledge of the electrostatic interactions can be used to control surface parameters such as surface concentration and surface diffusion, which intimately correlate with surface biocatalysis. We propose that the maximum reaction rate results from a balance between adsorption and surface diffusion. The above finding suggests enzyme engineering and process design strategies for improving interfacial biocatalysis in industrial, pharmaceutical, and food applications.

Enzyme immobilisation in biocatalysis: why, what and how.

PubMed

Sheldon, Roger A; van Pelt, Sander

2013-08-07

In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for immobilisation of enzymes as a key enabling technology for practical and commercial viability is emphasised. The underlying reasons for immobilisation are the need to improve the stability and recyclability of the biocatalyst compared to the free enzyme. The lower risk of product contamination with enzyme residues and low or no allergenicity are further advantages of immobilised enzymes. Methods for immobilisation are divided into three categories: adsorption on a carrier (support), encapsulation in a carrier, and cross-linking (carrier-free). General considerations regarding immobilisation, regardless of the method used, are immobilisation yield, immobilisation efficiency, activity recovery, enzyme loading (wt% in the biocatalyst) and the physical properties, e.g. particle size and density, hydrophobicity and mechanical robustness of the immobilisate, i.e. the immobilised enzyme as a whole (enzyme + support). The choice of immobilisate is also strongly dependent on the reactor configuration used, e.g. stirred tank, fixed bed, fluidised bed, and the mode of downstream processing. Emphasis is placed on relatively recent developments, such as the use of novel supports such as mesoporous silicas, hydrogels, and smart polymers, and cross-linked enzyme aggregates (CLEAs).

Streptomyces spp. in the biocatalysis toolbox.

PubMed

Spasic, Jelena; Mandic, Mina; Djokic, Lidija; Nikodinovic-Runic, Jasmina

2018-04-01

About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.

Representing metabolic pathway information: an object-oriented approach.

PubMed

Ellis, L B; Speedie, S M; McLeish, R

1998-01-01

The University of Minnesota Biocatalysis/Biodegradation Database (UM-BBD) is a website providing information and dynamic links for microbial metabolic pathways, enzyme reactions, and their substrates and products. The Compound, Organism, Reaction and Enzyme (CORE) object-oriented database management system was developed to contain and serve this information. CORE was developed using Java, an object-oriented programming language, and PSE persistent object classes from Object Design, Inc. CORE dynamically generates descriptive web pages for reactions, compounds and enzymes, and reconstructs ad hoc pathway maps starting from any UM-BBD reaction. CORE code is available from the authors upon request. CORE is accessible through the UM-BBD at: http://www. labmed.umn.edu/umbbd/index.html.

Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis.

PubMed

He, Yuanzhi; Chen, Feixue; Sun, Meijing; Gao, Huifang; Guo, Zewang; Lin, Hui; Chen, Jiebo; Jin, Wensong; Yang, Yunlong; Zhang, Liaoyuan; Yuan, Jun

2018-03-19

(3 S )-Acetoin and (2 S ,3 S )-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3 S )-acetoin and (2 S ,3 S )-2,3-butanediol from meso -2,3-butanediol. First, E. coli co-expressing (2 R ,3 R )-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3 S )-acetoin production from meso -2,3-butanediol. Maximum (3 S )-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions. Subsequently, we developed another biocatalyst co-expressing (2 S ,3 S )-2,3-butanediol dehydrogenase and formate dehydrogenase for (2 S ,3 S )-2,3-butanediol production from (3 S )-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2 S ,3 S )-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso -2,3-butanediol. These results exhibited the potential for (3 S )-acetoin and (2 S ,3 S )-butanediol production from meso -2,3-butanediol as a substrate via whole-cell biocatalysis.

Biodegradable products by lipase biocatalysis.

PubMed

Linko, Y Y; Lämsä, M; Wu, X; Uosukainen, E; Seppälä, J; Linko, P

1998-11-18

The interest in the applications of biocatalysis in organic syntheses has rapidly increased. In this context, lipases have recently become one of the most studied groups of enzymes. We have demonstrated that lipases can be used as biocatalyst in the production of useful biodegradable compounds. A number of examples are given. 1-Butyl oleate was produced by direct esterification of butanol and oleic acid to decrease the viscosity of biodiesel in winter use. Enzymic alcoholysis of vegetable oils without additional organic solvent has been little investigated. We have shown that a mixture of 2-ethyl-1-hexyl esters can be obtained in a good yield by enzymic transesterification from rapeseed oil fatty acids for use as a solvent. Trimethylolpropane esters were also similarly synthesized as lubricants. Finally, the discovery that lipases can also catalyze ester syntheses and transesterification reactions in organic solvent systems has opened up the possibility of enzyme catalyzed production of biodegradable polyesters. In direct polyesterification of 1,4-butanediol and sebacic acid, polyesters with a mass average molar mass of the order of 56,000 g mol-1 or higher, and a maximum molar mass of about 130,000 g mol-1 were also obtained by using lipase as biocatalyst. Finally, we have demonstrated that also aromatic polyesters can be synthesized by lipase biocatalysis, a higher than 50,000 g mol-1 mass average molar mass of poly(1,6-hexanediyl isophthalate) as an example.

Use of the University of Minnesota Biocatalysis/Biodegradation Database for study of microbial degradation

PubMed Central

2012-01-01

Microorganisms are ubiquitous on earth and have diverse metabolic transformative capabilities important for environmental biodegradation of chemicals that helps maintain ecosystem and human health. Microbial biodegradative metabolism is the main focus of the University of Minnesota Biocatalysis/Biodegradation Database (UM-BBD). UM-BBD data has also been used to develop a computational metabolic pathway prediction system that can be applied to chemicals for which biodegradation data is currently lacking. The UM-Pathway Prediction System (UM-PPS) relies on metabolic rules that are based on organic functional groups and predicts plausible biodegradative metabolism. The predictions are useful to environmental chemists that look for metabolic intermediates, for regulators looking for potential toxic products, for microbiologists seeking to understand microbial biodegradation, and others with a wide-range of interests. PMID:22587916

Phosphoketolase pathway engineering for carbon-efficient biocatalysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henard, Calvin Andrew; Freed, Emily Frances; Guarnieri, Michael Thomas

2015-12-01

Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Moreover, conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Here, we discuss recent advances and prospects formore » employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.« less

Spatial organization of multi-enzyme biocatalytic cascades.

PubMed

Quin, M B; Wallin, K K; Zhang, G; Schmidt-Dannert, C

2017-05-23

Industrial biocatalysis is an economically attractive option for the production of valuable chemicals. Our repertoire of cheap building blocks and commodity target molecules is vastly enhanced by multi-enzyme biocatalytic cascades. In order to achieve suitable titers in complex novel biocatalytic schemes, spatial organization may become necessary to overcome barriers caused by slow or inhibited enzymes as well as instability of biocatalysts. A number of spatial organization strategies are currently available, which could be integrated in the design of complex cascades. These include fusion proteins, immobilization on solid supports, multi-dimensional scaffolding, and encapsulation within vessels. This review article highlights recent advances in cascade biocatalysis, discusses the role of spatial organization in reaction kinetics, and presents some of the currently employed strategies for spatial organization of multi-enzyme cascades.

Fossil energy biotechnology: A research needs assessment. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-11-01

The Office of Program Analysis of the US Department of Energy commissioned this study to evaluate and prioritize research needs in fossil energy biotechnology. The objectives were to identify research initiatives in biotechnology that offer timely and strategic options for the more efficient and effective uses of the Nation`s fossil resource base, particularly the early identification of new and novel applications of biotechnology for the use or conversion of domestic fossil fuels. Fossil energy biotechnology consists of a number of diverse and distinct technologies, all related by the common denominator -- biocatalysis. The expert panel organized 14 technical subjects intomore » three interrelated biotechnology programs: (1) upgrading the fuel value of fossil fuels; (2) bioconversion of fossil feedstocks and refined products to added value chemicals; and, (3) the development of environmental management strategies to minimize and mitigate the release of toxic and hazardous petrochemical wastes.« less

Asymmetric reactions in continuous flow

PubMed Central

Mak, Xiao Yin; Laurino, Paola

2009-01-01

Summary An overview of asymmetric synthesis in continuous flow and microreactors is presented in this review. Applications of homogeneous and heterogeneous asymmetric catalysis as well as biocatalysis in flow are discussed. PMID:19478913

Introducing the concept of biocatalysis in the classroom: The conversion of cholesterol to provitamin D3.

PubMed

De Luca, Belén M; Nudel, Clara B; Gonzalez, Rodrigo H; Nusblat, Alejandro D

2017-03-04

Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course "design and optimization of biological systems" which is offered in the biochemistry curricula. We selected the ciliate tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D 3. The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties. © 2016 by The International Union of Biochemistry and Molecular Biology, 45(2):105-114, 2017. © 2016 The International Union of Biochemistry and Molecular Biology.

Structural DNA Nanotechnology: State of the Art and Future Perspective

PubMed Central

2015-01-01

Over the past three decades DNA has emerged as an exceptional molecular building block for nanoconstruction due to its predictable conformation and programmable intra- and intermolecular Watson–Crick base-pairing interactions. A variety of convenient design rules and reliable assembly methods have been developed to engineer DNA nanostructures of increasing complexity. The ability to create designer DNA architectures with accurate spatial control has allowed researchers to explore novel applications in many directions, such as directed material assembly, structural biology, biocatalysis, DNA computing, nanorobotics, disease diagnosis, and drug delivery. This Perspective discusses the state of the art in the field of structural DNA nanotechnology and presents some of the challenges and opportunities that exist in DNA-based molecular design and programming. PMID:25029570

Biocatalysis: Unmasked by stretching

NASA Astrophysics Data System (ADS)

Kharlampieva, Eugenia; Tsukruk, Vladimir V.

2009-09-01

The biocatalytic activity of enzyme-loaded responsive layer-by-layer films can be switched on and off by simple mechanical stretching. Soft materials could thus be used to trigger biochemical reactions under mechanical action, with potential therapeutic applications.

Technical and economic assessment of processes for the production of butanol and acetone. Phase two: analysis of research advances. Energy Conversion and Utilization Technologies Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1984-08-01

The initial objective of this work was to develop a methodology for analyzing the impact of technological advances as a tool to help establish priorities for R and D options in the field of biocatalysis. As an example of a biocatalyzed process, butanol/acetone fermentation (ABE process) was selected as the specific topic of study. A base case model characterizing the technology and economics associated with the ABE process was developed in the previous first phase of study. The project objectives were broadened in this second phase of work to provide parametric estimates of the economic and energy impacts of amore » variety of research advances in the hydrolysis, fermentation and purification sections of the process. The research advances analyzed in this study were based on a comprehensive literature review. The six process options analyzed were: continuous ABE fermentaton; vacuum ABE fermentation; Baelene solvent extraction; HRI's Lignol process; improved prehydrolysis/dual enzyme hydrolysis; and improved microorganism tolerance to butanol toxicity. Of the six options analyzed, only improved microorganism tolerance to butanol toxicity had a significant positive effect on energy efficiency and economics. This particular process option reduced the base case production cost (including 10% DCF return) by 20% and energy consumption by 16%. Figures and tables.« less

Non-aqueous homogenous biocatalytic conversion of polysaccharides in ionic liquids using chemically modified glucosidase.

PubMed

Brogan, Alex P S; Bui-Le, Liem; Hallett, Jason P

2018-06-25

The increasing requirement to produce platform chemicals and fuels from renewable sources means advances in biocatalysis are rapidly becoming a necessity. Biomass is widely used in nature as a source of energy and as chemical building blocks. However, recalcitrance towards traditional chemical processes and solvents provides a significant barrier to widespread utility. Here, by optimizing enzyme solubility in ionic liquids, we have discovered solvent-induced substrate promiscuity of glucosidase, demonstrating an unprecedented example of homogeneous enzyme bioprocessing of cellulose. Specifically, chemical modification of glucosidase for solubilization in ionic liquids can increase thermal stability to up to 137 °C, allowing for enzymatic activity 30 times greater than is possible in aqueous media. These results establish that through a synergistic combination of chemical biology (enzyme modification) and reaction engineering (solvent choice), the biocatalytic capability of enzymes can be intensified: a key step towards the full-scale deployment of industrial biocatalysis.

High-throughput strategies for the discovery and engineering of enzymes for biocatalysis.

PubMed

Jacques, Philippe; Béchet, Max; Bigan, Muriel; Caly, Delphine; Chataigné, Gabrielle; Coutte, François; Flahaut, Christophe; Heuson, Egon; Leclère, Valérie; Lecouturier, Didier; Phalip, Vincent; Ravallec, Rozenn; Dhulster, Pascal; Froidevaux, Rénato

2017-02-01

Innovations in novel enzyme discoveries impact upon a wide range of industries for which biocatalysis and biotransformations represent a great challenge, i.e., food industry, polymers and chemical industry. Key tools and technologies, such as bioinformatics tools to guide mutant library design, molecular biology tools to create mutants library, microfluidics/microplates, parallel miniscale bioreactors and mass spectrometry technologies to create high-throughput screening methods and experimental design tools for screening and optimization, allow to evolve the discovery, development and implementation of enzymes and whole cells in (bio)processes. These technological innovations are also accompanied by the development and implementation of clean and sustainable integrated processes to meet the growing needs of chemical, pharmaceutical, environmental and biorefinery industries. This review gives an overview of the benefits of high-throughput screening approach from the discovery and engineering of biocatalysts to cell culture for optimizing their production in integrated processes and their extraction/purification.

Improved strategies for electrochemical 1,4-NAD(P)H2 regeneration: A new era of bioreactors for industrial biocatalysis.

PubMed

Morrison, Clifford S; Armiger, William B; Dodds, David R; Dordick, Jonathan S; Koffas, Mattheos A G

Industrial enzymatic reactions requiring 1,4-NAD(P)H 2 to perform redox transformations often require convoluted coupled enzyme regeneration systems to regenerate 1,4-NAD(P)H 2 from NAD(P) and recycle the cofactor for as many turnovers as possible. Renewed interest in recycling the cofactor via electrochemical means is motivated by the low cost of performing electrochemical reactions, easy monitoring of the reaction progress, and straightforward product recovery. However, electrochemical cofactor regeneration methods invariably produce adventitious reduced cofactor side products which result in unproductive loss of input NAD(P). We review various literature strategies for mitigating adventitious product formation by electrochemical cofactor regeneration systems, and offer insight as to how a successful electrochemical bioreactor system could be constructed to engineer efficient 1,4-NAD(P)H 2 -dependent enzyme reactions of interest to the industrial biocatalysis community. Copyright © 2017 Elsevier Inc. All rights reserved.

Chemomimetic biocatalysis: exploiting the synthetic potential of cofactor-dependent enzymes to create new catalysts.

PubMed

Prier, Christopher K; Arnold, Frances H

2015-11-11

Despite the astonishing breadth of enzymes in nature, no enzymes are known for many of the valuable catalytic transformations discovered by chemists. Recent work in enzyme design and evolution, however, gives us good reason to think that this will change. We describe a chemomimetic biocatalysis approach that draws from small-molecule catalysis and synthetic chemistry, enzymology, and molecular evolution to discover or create enzymes with non-natural reactivities. We illustrate how cofactor-dependent enzymes can be exploited to promote reactions first established with related chemical catalysts. The cofactors can be biological, or they can be non-biological to further expand catalytic possibilities. The ability of enzymes to amplify and precisely control the reactivity of their cofactors together with the ability to optimize non-natural reactivity by directed evolution promises to yield exceptional catalysts for challenging transformations that have no biological counterparts.

News: Green Chemistry & Technology

EPA Science Inventory

A series of 21 articles focused on different features of green chemistry in a recent issue of Chemical Reviews. Topics extended over a wide range to include the design of sustainable synthetic processes to biocatalysis. A selection of perspectives follows as part of this colu

Biocatalysis for the production of industrial products and functional foods from rice and other agricultural produce.

PubMed

Akoh, Casimir C; Chang, Shu-Wei; Lee, Guan-Chiun; Shaw, Jei-Fu

2008-11-26

Many industrial products and functional foods can be obtained from cheap and renewable raw agricultural materials. For example, starch can be converted to bioethanol as biofuel to reduce the current demand for petroleum or fossil fuel energy. On the other hand, starch can also be converted to useful functional ingredients, such as high fructose and high maltose syrups, wine, glucose, and trehalose. The conversion process involves fermentation by microorganisms and use of biocatalysts such as hydrolases of the amylase superfamily. Amylases catalyze the process of liquefaction and saccharification of starch. It is possible to perform complete hydrolysis of starch by using the fusion product of both linear and debranching thermostable enzymes. This will result in saving energy otherwise needed for cooling before the next enzyme can act on the substrate, if a sequential process is utilized. Recombinant enzyme technology, protein engineering, and enzyme immobilization are powerful tools available to enhance the activity of enzymes, lower the cost of enzyme through large scale production in a heterologous host, increase their thermostability, improve pH stability, enhance their productivity, and hence making it competitive with the chemical processes involved in starch hydrolysis and conversions. This review emphasizes the potential of using biocatalysis for the production of useful industrial products and functional foods from cheap agricultural produce and transgenic plants. Rice was selected as a typical example to illustrate many applications of biocatalysis in converting low-value agricultural produce to high-value commercial food and industrial products. The greatest advantages of using enzymes for food processing and for industrial production of biobased products are their environmental friendliness and consumer acceptance as being a natural process.

Systems Biocatalysis: Development and engineering of cell-free "artificial metabolisms" for preparative multi-enzymatic synthesis.

PubMed

Fessner, Wolf-Dieter

2015-12-25

Systems Biocatalysis is an emerging concept of organizing enzymes in vitro to construct complex reaction cascades for an efficient, sustainable synthesis of valuable chemical products. The strategy merges the synthetic focus of chemistry with the modular design of biological systems, which is similar to metabolic engineering of cellular production systems but can be realized at a far lower level of complexity from a true reductionist approach. Such operations are free from material erosion by competing metabolic pathways, from kinetic restrictions by physical barriers and regulating circuits, and from toxicity problems with reactive foreign substrates, which are notorious problems in whole-cell systems. A particular advantage of cell-free concepts arises from the inherent opportunity to construct novel biocatalytic reaction systems for the efficient synthesis of non-natural products ("artificial metabolisms") by using enzymes specifically chosen or engineered for non-natural substrate promiscuity. Examples illustrating the technology from our laboratory are discussed. Copyright © 2014 Elsevier B.V. All rights reserved.

Enzymatic synthesis of novel phloretin glucosides.

PubMed

Pandey, Ramesh Prasad; Li, Tai Feng; Kim, Eun-Hee; Yamaguchi, Tokutaro; Park, Yong Il; Kim, Joong Su; Sohng, Jae Kyung

2013-06-01

A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.

Enzymatic Synthesis of Novel Phloretin Glucosides

PubMed Central

Pandey, Ramesh Prasad; Li, Tai Feng; Kim, Eun-Hee; Yamaguchi, Tokutaro; Park, Yong Il; Kim, Joong Su

2013-01-01

A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli. PMID:23542617

ECUT: Energy Conversion and Utilization Technologies program biocatalysis research activity. Potential membrane applications to biocatalyzed processes: Assessment of concentration polarization and membrane fouling

NASA Technical Reports Server (NTRS)

Ingham, J. D.

1983-01-01

Separation and purification of the products of biocatalyzed fermentation processes, such as ethanol or butanol, consumes most of the process energy required. Since membrane systems require substantially less energy for separation than most alternatives (e.g., distillation) they have been suggested for separation or concentration of fermentation products. This report is a review of the effects of concentration polarization and membrane fouling for the principal membrane processes: microfiltration, ultrafiltration, reverse osmosis, and electrodialysis including a discussion of potential problems relevant to separation of fermentation products. It was concluded that advanced membrane systems may result in significantly decreased energy consumption. However, because of the need to separate large amounts of water from much smaller amounts of product that may be more volatile than wate, it is not clear that membrane separations will necessarily be more efficient than alternative processes.

Proteins as templates for complex synthetic metalloclusters: towards biologically programmed heterogeneous catalysis

PubMed Central

Fehl, Charlie

2016-01-01

Despite nature’s prevalent use of metals as prosthetics to adapt or enhance the behaviour of proteins, our ability to programme such architectural organization remains underdeveloped. Multi-metal clusters buried in proteins underpin the most remarkable chemical transformations in nature, but we are not yet in a position to fully mimic or exploit such systems. With the advent of copious, relevant structural information, judicious mechanistic studies and the use of accessible computational methods in protein design coupled with new synthetic methods for building biomacromolecules, we can envisage a ‘new dawn’ that will allow us to build de novo metalloenzymes that move beyond mono-metal centres. In particular, we highlight the need for systems that approach the multi-centred clusters that have evolved to couple electron shuttling with catalysis. Such hybrids may be viewed as exciting mid-points between homogeneous and heterogeneous catalysts which also exploit the primary benefits of biocatalysis. PMID:27279776

Biotechnology and genetic engineering in the new drug development. Part III. Biocatalysis, metabolic engineering and molecular modelling.

PubMed

Stryjewska, Agnieszka; Kiepura, Katarzyna; Librowski, Tadeusz; Lochyński, Stanisław

2013-01-01

Industrial biotechnology has been defined as the use and application of biotechnology for the sustainable processing and production of chemicals, materials and fuels. It makes use of biocatalysts such as microbial communities, whole-cell microorganisms or purified enzymes. In the review these processes are described. Drug design is an iterative process which begins when a chemist identifies a compound that displays an interesting biological profile and ends when both the activity profile and the chemical synthesis of the new chemical entity are optimized. Traditional approaches to drug discovery rely on a stepwise synthesis and screening program for large numbers of compounds to optimize activity profiles. Over the past ten to twenty years, scientists have used computer models of new chemical entities to help define activity profiles, geometries and relativities. This article introduces inter alia the concepts of molecular modelling and contains references for further reading.

Biocatalysis with Sol-Gel Encapsulated Acid Phosphatase

ERIC Educational Resources Information Center

Kulkarni, Suhasini; Tran, Vu; Ho, Maggie K.-M.; Phan, Chieu; Chin, Elizabeth; Wemmer, Zeke; Sommerhalter, Monika

2010-01-01

This experiment was performed in an upper-level undergraduate biochemistry laboratory course. Students learned how to immobilize an enzyme in a sol-gel matrix and how to perform and evaluate enzyme-activity measurements. The enzyme acid phosphatase (APase) from wheat germ was encapsulated in sol-gel beads that were prepared from the precursor…

Biocatalyzed Regioselective Synthesis in Undergraduate Organic Laboratories: Multistep Synthesis of 2-Arachidonoylglycerol

ERIC Educational Resources Information Center

Johnston, Meghan R.; Makriyannis, Alexandros; Whitten, Kyle M.; Drew, Olivia C.; Best, Fiona A.

2016-01-01

In order to introduce the concepts of biocatalysis and its utility in synthesis to organic chemistry students, a multistep synthesis of endogenous cannabinergic ligand 2-arachidonoylglycerol (2-AG) was tailored for use as a laboratory exercise. Over four weeks, students successfully produced 2-AG, purifying and characterizing products at each…

Co-production of bio-ethanol, xylonic acid and slow-release nitrogen fertilizer from low-cost straw pulping solid residue.

PubMed

Huang, Chen; Ragauskas, Arthur J; Wu, Xinxing; Huang, Yang; Zhou, Xuelian; He, Juan; Huang, Caoxing; Lai, Chenhuan; Li, Xin; Yong, Qiang

2018-02-01

A novel bio-refinery sequence yielding varieties of co-products was developed using straw pulping solid residue. This process utilizes neutral sulfite pretreatment which under optimal conditions (160 °C and 3% (w/v) sulfite charge) provides 64.3% delignification while retaining 90% of cellulose and 67.3% of xylan. The pretreated solids exhibited excellent enzymatic digestibility, with saccharification yields of 86.9% and 81.1% for cellulose and xylan, respectively. After pretreatment, the process of semi-simultaneous saccharification and fermentation (S-SSF) and bio-catalysis was investigated. The results revealed that decreased ethanol yields were achieved when solid loading increased from 5% to 30%. An acceptable ethanol yield of 76.8% was obtained at 20% solid loading. After fermentation, bio-catalysis of xylose remaining in fermentation broth resulted in near 100% xylonic acid (XA) yield at varied solid loadings. To complete the co-product portfolio, oxidation ammoniation of the dissolved lignin successfully transformed it into biodegradable slow-release nitrogen fertilizer with excellent agricultural properties. Copyright © 2017 Elsevier Ltd. All rights reserved.

Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis

PubMed Central

French, Nigel G.; Scoble, Judith A.; Williams, Charlotte C.; Churches, Quentin I.; Frazer, Andrew R.; Taylor, Matthew C.; Coia, Greg; Simpson, Gregory; Turner, Nicholas J.; Scott, Colin

2017-01-01

Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formation with the generation of two new stereo-centres, with access to all four possible stereoisomers of a compound. Dihydroxyacetone phosphate (DHAP) is unstable so the provision of DHAP for DHAP-dependent aldolases in biocatalytic processes remains complicated. Our research has investigated the efficiency of several different enzymatic cascades for the conversion of glycerol to DHAP, including characterising new candidate enzymes for some of the reaction steps. The most efficient cascade for DHAP production, comprising a one-pot four-enzyme reaction with glycerol kinase, acetate kinase, glycerophosphate oxidase and catalase, was coupled with a DHAP-dependent fructose-1,6-biphosphate aldolase enzyme to demonstrate the production of several rare chiral sugars. The limitation of batch biocatalysis for these reactions and the potential for improvement using kinetic modelling and flow biocatalysis systems is discussed. PMID:29112947

Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis.

PubMed

Hartley, Carol J; French, Nigel G; Scoble, Judith A; Williams, Charlotte C; Churches, Quentin I; Frazer, Andrew R; Taylor, Matthew C; Coia, Greg; Simpson, Gregory; Turner, Nicholas J; Scott, Colin

2017-01-01

Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formation with the generation of two new stereo-centres, with access to all four possible stereoisomers of a compound. Dihydroxyacetone phosphate (DHAP) is unstable so the provision of DHAP for DHAP-dependent aldolases in biocatalytic processes remains complicated. Our research has investigated the efficiency of several different enzymatic cascades for the conversion of glycerol to DHAP, including characterising new candidate enzymes for some of the reaction steps. The most efficient cascade for DHAP production, comprising a one-pot four-enzyme reaction with glycerol kinase, acetate kinase, glycerophosphate oxidase and catalase, was coupled with a DHAP-dependent fructose-1,6-biphosphate aldolase enzyme to demonstrate the production of several rare chiral sugars. The limitation of batch biocatalysis for these reactions and the potential for improvement using kinetic modelling and flow biocatalysis systems is discussed.

Thermophilic enzymes and their applications in biocatalysis: a robust aldo-keto reductase.

PubMed

Willies, Simon; Isupov, Misha; Littlechild, Jennifer

2010-09-01

Extremophiles are providing a good source of novel robust enzymes for use in biocatalysis for the synthesis of new drugs. This is particularly true for the enzymes from thermophilic organisms which are more robust than their mesophilic counterparts to the conditions required for industrial bio-processes. This paper describes a new aldo-keto reductase enzyme from a thermophilic eubacteria, Thermotoga maritima which can be used for the production of primary alcohols. The enzyme has been cloned and over-expressed in Escherichia coli and has been purified and subjected to full biochemical characterization. The aldo-keto reductase can be used for production of primary alcohols using substrates including benzaldehyde, 1,2,3,6-tetrahydrobenzaldehyde and para-anisaldehyde. It is stable up to 80 degrees C, retaining over 60% activity for 5 hours at this temperature. The enzyme at pH 6.5 showed a preference for the forward, carbonyl reduction. The enzyme showed moderate stability with organic solvents, and retained 70% activity in 20% (v/v) isopropanol or DMSO. These properties are favourable for its potential industrial applications.

Biodiesel production from triolein and short chain alcohols through biocatalysis.

PubMed

Salis, Andrea; Pinna, Marcella; Monduzzi, Maura; Solinas, Vincenzo

2005-09-29

Oleic acid alkyl esters (biodiesel) were synthesised by biocatalysis in solvent-free conditions. Different commercial immobilised lipases, namely Candida antarctica B, Rizhomucor miehei, and Pseudomonas cepacia, were tested towards the reaction between triolein and butanol to produce butyl oleate. Pseudomonas cepacia lipase resulted to be the most active enzyme reaching 100% of conversion after 6h. Different operative conditions such as reaction temperature, water activity, and reagent stoichiometric ratio were investigated and optimised. These conditions were then used to investigate the effect of linear and branched short chain alcohols. Methanol and 2-butanol were the worst alcohols: the former, probably, due to its low miscibility with the oil and the latter because secondary alcohols usually are less reactive than primary alcohols. Conversely, linear and branched primary alcohols with short alkyl chains (C(2)--C(4)) showed high reaction rate and conversion. A mixture of linear and branched short chain alcohols that mimics the residual of ethanol distillation (fusel oil) was successfully used for oleic acid ester synthesis. These compounds are important in biodiesel mixtures since they improve low temperature properties.

Enzyme-Embedded, Microstructural Reactors for Industrial Biocatalysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baker, Sarah E.; Knipe, J. M.; Oakdale, J.

In this project we explored enzyme-catalyzed methane conversion to methanol. Industrial biological approaches to methane conversion using whole organisms are predicted to be more energy efficient than chemical approaches, but are limited by mass transfer of the gas phase reactants, methane and oxygen, to the organisms. We demonstrated that 3D printing the enzyme particulate Methane Mono Oxygenase (pMMO) embedded in a polymer can improve the kinetics of methane to methanol conversion. This improvement was likely due to the ability to increase the surface area of the catalytic material using 3D printing. We also demonstrated the first continuous use of pMMOmore » in a flow-through reactor. In order to understand the fundamental kinetic properties of pMMO, we conducted an in-depth study of pMMO kinetics using analytical tools developed in our lab. Finally, we developed a new copolymer system that allowed tuning of the gas permeability of the biocatalytic material.« less

Generic DART-MS platform for monitoring the on-demand continuous-flow production of pharmaceuticals: Advancing the quantitative protocol for caffeates in microfluidic biocatalysis.

PubMed

Xu, Yan; Zhang, Dong-Yang; Meng, Xiang-Yun; Liu, Xi; Sheng, Sheng; Wu, Guo-Hua; Wang, Jun; Wu, Fu-An

2017-04-15

Today, continuous processing is regarded as an effective on-demand production technique of pharmaceuticals. Homemade microreactors packed with immobilized lipase under continuous-flow conditions were first applied to tailor the production of high-value caffeic acid phenethyl ester (CAPE) from methyl caffeate (MC) and 2-phenylethanol (PE) in cyclohexane via transesterification; however, this method is challenging due to the lack of a rapid platform for monitoring caffeates in microfluidic biocatalysis. The reactants were directly analyzed using Direct Analysis in Real Time Mass Spectrometry (DART-MS), and the corresponding ionization parameters were investigated. Special ions produced from MC (parent ion m/z 192.87 and product ion m/z 133.44) and CAPE (parent ion m/z 282.93 and product ion m/z 178.87) were determined using DART-MS 2 in the negative ion mode. The peak areas of the select reaction monitoring (SRM) signals were calculated to develop the standard curves for quantitative analyses of the concentration. Reasonable linear regression equations of MC and CAPE were obtained in the range of 3.125-50.000mg/L, with linear coefficients (R 2 ) of 0.9515 and 0.9973, limits of detection (LOD) of 0.005 and 0.003mg/L, limits of quantification (LOQ) of 0.02 and 0.01mg/L, and recovery ranges of 92.50-97.11% and 90.11-97.60%, respectively. The results using DART-MS 2 were in good agreement with those using conventional High-Performance Liquid Chromatography with a UV detector (HPLC-UV) and were successfully applied to monitor the kinetics constants and mass transfer coefficients in a continuous-flow packed bed microreactor. Thus, the DART-MS 2 method is an efficient tool for analyzing caffeates in microfluidic biocatalysis with limited sample preparation and short operating time. Copyright © 2017 Elsevier B.V. All rights reserved.

Draft Genome Sequence of Methylomicrobium buryatense Strain 5G, a Haloalkaline-Tolerant Methanotrophic Bacterium

PubMed Central

Khmelenina, Valentina N.; Beck, David A. C.; Munk, Christine; Davenport, Karen; Daligault, Hajnalka; Erkkila, Tracy; Goodwin, Lynne; Gu, Wei; Lo, Chien-Chi; Scholz, Matthew; Teshima, Hazuki; Xu, Yan; Chain, Patrick; Bringel, Francoise; Vuilleumier, Stéphane; DiSpirito, Alan; Dunfield, Peter; Jetten, Mike S. M.; Klotz, Martin G.; Knief, Claudia; Murrell, J. Colin; Op den Camp, Huub J. M.; Sakai, Yasuyoshi; Semrau, Jeremy; Svenning, Mette; Stein, Lisa Y.; Trotsenko, Yuri A.

2013-01-01

Robust growth of the gammaproteobacterium Methylomicrobium buryatense strain 5G on methane makes it an attractive system for CH4-based biocatalysis. Here we present a draft genome sequence of the strain that will provide a valuable framework for metabolic engineering of the core pathways for the production of valuable chemicals from methane. PMID:23814105

Biosilica-Immobilized Enzymes for Biocatalysis (Preprint)

DTIC Science & Technology

2007-08-01

strengthening of dough Lipases, Proteases Accelerates cheese ripening Glucose Isomerase Production of high fructose corn syrup Pectinases Fruit juice...Manufacture of glucose syrups and starch modification Maltogenic alpha-amylase Improves shelf life of bread Lipoxygenase Used for bleaching and...such as pH, ionic strength or temperature. In any approach to immobilize enzymes, the goal is to achieve a high specific activity without

Expression of a Dianthus flavonoid glucosyltransferase in Saccharomyces cerevisiae for whole-cell biocatalysis.

PubMed

Werner, Sean R; Morgan, John A

2009-07-15

Glycosyltransferases are promising biocatalysts for the synthesis of small molecule glycosides. In this study, Saccharomyces cerevisiae expressing a flavonoid glucosyltransferase (GT) from Dianthus caryophyllus (carnation) was investigated as a whole-cell biocatalyst. Two yeast expression systems were compared using the flavonoid naringenin as a model substrate. Under in vitro conditions, naringenin-7-O-glucoside was formed and a higher specific glucosyl transfer activity was found using a galactose inducible expression system compared to a constitutive expression system. However, S. cerevisiae expressing the GT constitutively was significantly more productive than the galactose inducible system under in vivo conditions. Interestingly, the glycosides were recovered directly from the culture broth and did not accumulate intracellularly. A previously uncharacterized naringenin glycoside formed using the D. caryophyllus GT was identified as naringenin-4'-O-glucoside. It was found that S. cerevisiae cells hydrolyze naringenin-7-O-glucoside during whole-cell biocatalysis, resulting in a low final glycoside titer. When phloretin was added as a substrate to the yeast strain expressing the GT constitutively, the natural product phlorizin was formed. This study demonstrates S. cerevisiae is a promising whole-cell biocatalyst host for the production of valuable glycosides.

Acceleration of Aglycone Isoflavone and γ-Aminobutyric Acid Production from Doenjang Using Whole-Cell Biocatalysis Accompanied by Protease Treatment.

PubMed

Li, Yincong; Ku, Seockmo; Park, Myeong Soo; Li, Zhipeng; Ji, Geun Eog

2017-11-28

Recently, soybean isoflavone aglycones ( i.e. , daidzein and genistein) and γ-aminobutyric acid (GABA) have begun to receive considerable consumer attention owing to their potential as nutraceuticals. To produce these ingredients, multiple microorganisms and their enzymes are commonly used for catalysis in the nutraceutical industry. In this work, we introduce a novel fermentation process that uses whole-cell biocatalysis to accelerate GABA and isoflavone aglycone production in doenjang (a traditional Korean soybean paste). Microbial enzymes transform soybean isoflavone glycosides ( i.e. , daidzin and genistin) and monosodium glutamate into soybean isoflavone aglycones and GABA. Lactobacillus brevis GABA 100 and Aspergillus oryzae KACC 40250 significantly reduced the production time with the aid of a protease. The resulting levels of GABA and daidzein were higher, and genistein production resembled the levels in traditional doenjang fermented for over a year. Concentrations of GABA, daidzein, and genistein were measured as 7,162, 60, and 59 μg/g, respectively on the seventh day of fermentation. Our results demonstrate that the administration of whole-cell L. brevis GABA 100 and A. oryzae KACC 40250 paired with a protease treatment is an effective method to accelerate GABA, daidzein, and genistein production in doenjang.

Metabolic engineering is key to a sustainable chemical industry.

PubMed

Murphy, Annabel C

2011-08-01

The depletion of fossil fuel stocks will prohibit their use as the main feedstock of future industrial processes. Biocatalysis is being increasingly used to reduce fossil fuel reliance and to improve the sustainability, efficiency and cost of chemical production. Even with their current small market share, biocatalyzed processes already generate approximately US$50 billion and it has been estimated that they could be used to produce up to 20% of fine chemicals by 2020. Until the advent of molecular biological technologies, the compounds that were readily accessible from renewable biomass were restricted to naturally-occurring metabolites. However, metabolic engineering has considerably broadened the range of compounds now accessible, providing access to compounds that cannot be otherwise reliably sourced, as well as replacing established chemical processes. This review presents the case for continued efforts to promote the adoption of biocatalyzed processes, highlighting successful examples of industrial chemical production from biomass and/or via biocatalyzed processes. A selection of emerging technologies that may further extend the potential and sustainability of biocatalysis are also presented. As the field matures, metabolic engineering will be increasingly crucial in maintaining our quality of life into a future where our current resources and feedstocks cannot be relied upon.

Are Lipases Still Important Biocatalysts? A Study of Scientific Publications and Patents for Technological Forecasting

PubMed Central

Daiha, Karina de Godoy; Angeli, Renata; de Oliveira, Sabrina Dias; Almeida, Rodrigo Volcan

2015-01-01

The great potential of lipases is known since 1930 when the work of J. B. S. Haldane was published. After eighty-five years of studies and developments, are lipases still important biocatalysts? For answering this question the present work investigated the technological development of four important industrial sectors where lipases are applied: production of detergent formulations; organic synthesis, focusing on kinetic resolution, production of biodiesel, and production of food and feed products. The analysis was made based on research publications and patent applications, working as scientific and technological indicators, respectively. Their evolution, interaction, the major players of each sector and the main subject matters disclosed in patent documents were discussed. Applying the concept of technology life cycle, S-curves were built by plotting cumulative patent data over time to monitor the attractiveness of each technology for investment. The results lead to a conclusion that the use of lipases as biocatalysts is still a relevant topic for the industrial sector, but developments are still needed for lipase biocatalysis to reach its full potential, which are expected to be achieved within the third, and present, wave of biocatalysis. PMID:26111144

Biocatalysis with thermostable enzymes: structure and properties of a thermophilic 'ene'-reductase related to old yellow enzyme.

PubMed

Adalbjörnsson, Björn V; Toogood, Helen S; Fryszkowska, Anna; Pudney, Christopher R; Jowitt, Thomas A; Leys, David; Scrutton, Nigel S

2010-01-25

We report the crystal structure of a thermophilic "ene" reductase (TOYE) isolated from Thermoanaerobacter pseudethanolicus E39. The crystal structure reveals a tetrameric enzyme and an active site that is relatively large compared to most other structurally determined and related Old Yellow Enzymes. The enzyme adopts higher order oligomeric states (octamers and dodecamers) in solution, as revealed by sedimentation velocity and multiangle laser light scattering. Bead modelling indicates that the solution structure is consistent with the basic tetrameric structure observed in crystallographic studies and electron microscopy. TOYE is stable at high temperatures (T(m)>70 degrees C) and shows increased resistance to denaturation in water-miscible organic solvents compared to the mesophilic Old Yellow Enzyme family member, pentaerythritol tetranitrate reductase. TOYE has typical ene-reductase properties of the Old Yellow Enzyme family. There is currently major interest in using Old Yellow Enzyme family members in the preparative biocatalysis of a number of activated alkenes. The increased stability of TOYE in organic solvents is advantageous for biotransformations in which water-miscible organic solvents and biphasic reaction conditions are required to both deliver novel substrates and minimize product racemisation.

Biocatalytic synthesis of 4-pregnen-20,21-diol-3-one, a selective inhibitor of human 5alpha-reductase type II.

PubMed

Hannemann, Frank; Bernhardt, Rita; Jose, Joachim

2007-10-01

Biocatalysis, the conversion of substrates into valuable products by the use of enzymes, has some striking advantages in comparison to standard organic chemistry for drug synthesis. By biocatalysis, substrates that contain several identical reactive groups at different positions can be converted with high regio-selectivity and enantio-selectivity. In this study, an E. coli isolate (E132) was identified which was able to convert the steroid desoxycorticosterone into the product 4-pregnen-20,21-diol-3-one in real terms. The product was purified from the cell culture supernatant by HPLC and its structure was demonstrated by mass spectrometry and NMR spectroscopy. It was tested on inhibition of human 5alpha-reductases type I and type II. At a concentration of 10 microM, inhibition was 49.0% for type I and 81.8% for type II, whereas there was no inhibition of human aromatase (CYP19) at 20 microM and human 17alpha-hydroxylase-C17,20-lyase (CYP17) at 2.5 microM detectable. The IC50 value of 4-pregnen-20,21-diol-3-one for human 5alpha-reductase type II was determined to be 1.56 microM.

Are Lipases Still Important Biocatalysts? A Study of Scientific Publications and Patents for Technological Forecasting.

PubMed

Daiha, Karina de Godoy; Angeli, Renata; de Oliveira, Sabrina Dias; Almeida, Rodrigo Volcan

2015-01-01

The great potential of lipases is known since 1930 when the work of J. B. S. Haldane was published. After eighty-five years of studies and developments, are lipases still important biocatalysts? For answering this question the present work investigated the technological development of four important industrial sectors where lipases are applied: production of detergent formulations; organic synthesis, focusing on kinetic resolution, production of biodiesel, and production of food and feed products. The analysis was made based on research publications and patent applications, working as scientific and technological indicators, respectively. Their evolution, interaction, the major players of each sector and the main subject matters disclosed in patent documents were discussed. Applying the concept of technology life cycle, S-curves were built by plotting cumulative patent data over time to monitor the attractiveness of each technology for investment. The results lead to a conclusion that the use of lipases as biocatalysts is still a relevant topic for the industrial sector, but developments are still needed for lipase biocatalysis to reach its full potential, which are expected to be achieved within the third, and present, wave of biocatalysis.

Enantioselective Reduction by Crude Plant Parts: Reduction of Benzofuran-2-yl Methyl Ketone with Carrot ("Daucus carota") Bits

ERIC Educational Resources Information Center

Ravia, Silvana; Gamenara, Daniela; Schapiro, Valeria; Bellomo, Ana; Adum, Jorge; Seoane, Gustavo; Gonzalez, David

2006-01-01

The use of biocatalysis and biotransformations are important tools in green chemistry. The enantioselective reduction of a ketone by crude plant parts, using carrot ("Daucus carota") as the reducing agent is presented. The experiment introduces an example of a green chemistry procedure that can be tailored to fit in a regular laboratory session.…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pruski, Marek; Sadow, Aaron D.; Slowing, Igor I.

Here the catalysis research at the U.S. Department of Energy's (DOE's) National Laboratories covers a wide range of research topics in heterogeneous catalysis, homogeneous/molecular catalysis, biocatalysis, electrocatalysis, and surface science. Since much of the work at National Laboratories is funded by DOE, the research is largely focused on addressing DOE's mission to ensure America's security and prosperity by addressing its energy, environmental, and nuclear challenges through transformative science and technology solutions.

COLLABORATIVE RESEARCH AND DEVELOPMENT CONTRACT. Delivery Order 0038: Microbial Biotechnology and Biocatalysis

DTIC Science & Technology

2010-04-01

encapsulated lysozyme. Bacteriolytic activity was inves- tigated with Micrococcus lysodeikticus cells and by hydroly- sis of a synthetic membrane mimic...immobilized lysozyme in silica or tita- nia nanoparticles. Lysozyme activity determined by lytic assay with Micrococcus lysodeikticus cells (&) or with...voltage of 200 V and according to the manufacturer’s instruc- tions. Lysozyme activity assays were performed with Micrococcus lysodeikticus cells

Nanoparticle cages for enzyme catalysis in organic media.

PubMed

Wu, Changzhu; Bai, Shuo; Ansorge-Schumacher, Marion B; Wang, Dayang

2011-12-15

Encapsulation of enzymes in Pickering emulsions results in a large interfacial area of the enzyme-containing aqueous phase for biocatalysis in organic media. This immobilization technique minimizes enzyme inactivation through stabilizing immiscible liquids by particles, facilitates separation processes, and significantly increases catalytic performance of both stable and vulnerable enzymes. Thus, a broad technical applicability can be envisioned. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

[Polyketone Reaction in Biosynthetic Pathways of 2, 3, 5, 4'-Tetrahydroxy Stilhene-2-O-β-D-glucoside in Polygonum multiflorum by Biocatalysis].

PubMed

Lei, Lei; Xia, Wan-xia; Shao, Li; Zhao, Shu-jin

2015-10-01

2, 3, 5, 4'-Tetrahydroxy stilbene-2-O-β-D-glucoside (THSG), the active ingredient of Polygonum multiflorum, its polyketone reaction in the biosynthesis pathways was studied by biocatalysis method. The substrates 4-coumaroyl-CoA and malonyl-CoA were catalyzed in vitro by the crude enzyme extracted from Polygonum multiflorum callus, then the products were verified by HPLC and LC-MS methods. And the crude enzyme was analyzed by ammonium sulfate precipitation method and SDS-PAGE. HPLC chromatogram showed the same retention time of both the product and resveratrol standards; LC-MS spectra showed that the m/z of product was 227, which was consistent with resveratrol standards under the mode of negative ion; Ammonium sulfate (AS) precipitation method showed AS of 40% - 70% had catalytic activity,and 50% - 60% was the optimum; SDS-PAGE showed protein bands were obviously different among different AS concentration between 20% - 80%, the protein band of 42 kDa was found in AS of 50% - 60%, which had the same molecular weight with stilbene synthase. The product of polyketone reaction in the biosynthesis of THSG is resveratrol rather than THSG, so it is speculated that THSG is the conversion product of resveratrol instead of the direct product of the polyketone reaction.

Strategies for discovery and improvement of enzyme function: state of the art and opportunities

PubMed Central

Kaul, Praveen; Asano, Yasuhisa

2012-01-01

Summary Developments in biocatalysis have been largely fuelled by consumer demands for new products, industrial attempts to improving existing process and minimizing waste, coupled with governmental measures to regulate consumer safety along with scientific advancements. One of the major hurdles to application of biocatalysis to chemical synthesis is unavailability of the desired enzyme to catalyse the reaction to allow for a viable process development. Even when the desired enzyme is available it often forces the process engineers to alter process parameters due to inadequacies of the enzyme, such as instability, inhibition, low yield or selectivity, etc. Developments in the field of enzyme or reaction engineering have allowed access to means to achieve the ends, such as directed evolution, de novo protein design, use of non‐conventional media, using new substrates for old enzymes, active‐site imprinting, altering temperature, etc. Utilization of enzyme discovery and improvement tools therefore provides a feasible means to overcome this problem. Judicious employment of these tools has resulted in significant advancements that have leveraged the research from laboratory to market thus impacting economic growth; however, there are further opportunities that have not yet been explored. The present review attempts to highlight some of these achievements and potential opportunities. PMID:21883976

Evaluating the Development of Biocatalytic Technology for the Targeted Removal of Perchlorate from Drinking Water.

PubMed

Hutchison, Justin M; Guest, Jeremy S; Zilles, Julie L

2017-06-20

Removing micropollutants is challenging in part because of their toxicity at low concentrations. A biocatalytic approach could harness the high affinity of enzymes for their substrates to address this challenge. The potential of biocatalysis relative to mature (nonselective ion exchange, selective ion exchange, and whole-cell biological reduction) and emerging (catalysis) perchlorate-removal technologies was evaluated through a quantitative sustainable design framework, and research objectives were prioritized to advance economic and environmental sustainability. In its current undeveloped state, the biocatalytic technology was approximately 1 order of magnitude higher in cost and environmental impact than nonselective ion exchange. Biocatalyst production was highly correlated with cost and impact. Realistic improvement scenarios targeting biocatalyst yield, biocatalyst immobilization for reuse, and elimination of an electron shuttle could reduce total costs to $0.034 m -3 and global warming potential (GWP) to 0.051 kg CO 2 eq m -3 : roughly 6.5% of cost and 7.3% of GWP of the background from drinking water treatment and competitive with the best performing technology, selective ion exchange. With less stringent perchlorate regulatory limits, ion exchange technologies had increased cost and impact, in contrast to biocatalytic and catalytic technologies. Targeted advances in biocatalysis could provide affordable and sustainable treatment options to protect the public from micropollutants.

Mechanistic modeling of biocorrosion caused by biofilms of sulfate reducing bacteria and acid producing bacteria.

PubMed

Xu, Dake; Li, Yingchao; Gu, Tingyue

2016-08-01

Biocorrosion is also known as microbiologically influenced corrosion (MIC). Most anaerobic MIC cases can be classified into two major types. Type I MIC involves non-oxygen oxidants such as sulfate and nitrate that require biocatalysis for their reduction in the cytoplasm of microbes such as sulfate reducing bacteria (SRB) and nitrate reducing bacteria (NRB). This means that the extracellular electrons from the oxidation of metal such as iron must be transported across cell walls into the cytoplasm. Type II MIC involves oxidants such as protons that are secreted by microbes such as acid producing bacteria (APB). The biofilms in this case supply the locally high concentrations of oxidants that are corrosive without biocatalysis. This work describes a mechanistic model that is based on the biocatalytic cathodic sulfate reduction (BCSR) theory. The model utilizes charge transfer and mass transfer concepts to describe the SRB biocorrosion process. The model also includes a mechanism to describe APB attack based on the local acidic pH at a pit bottom. A pitting prediction software package has been created based on the mechanisms. It predicts long-term pitting rates and worst-case scenarios after calibration using SRB short-term pit depth data. Various parameters can be investigated through computer simulation. Copyright © 2016 Elsevier B.V. All rights reserved.

Strategies for discovery and improvement of enzyme function: state of the art and opportunities.

PubMed

Kaul, Praveen; Asano, Yasuhisa

2012-01-01

Developments in biocatalysis have been largely fuelled by consumer demands for new products, industrial attempts to improving existing process and minimizing waste, coupled with governmental measures to regulate consumer safety along with scientific advancements. One of the major hurdles to application of biocatalysis to chemical synthesis is unavailability of the desired enzyme to catalyse the reaction to allow for a viable process development. Even when the desired enzyme is available it often forces the process engineers to alter process parameters due to inadequacies of the enzyme, such as instability, inhibition, low yield or selectivity, etc. Developments in the field of enzyme or reaction engineering have allowed access to means to achieve the ends, such as directed evolution, de novo protein design, use of non-conventional media, using new substrates for old enzymes, active-site imprinting, altering temperature, etc. Utilization of enzyme discovery and improvement tools therefore provides a feasible means to overcome this problem. Judicious employment of these tools has resulted in significant advancements that have leveraged the research from laboratory to market thus impacting economic growth; however, there are further opportunities that have not yet been explored. The present review attempts to highlight some of these achievements and potential opportunities. © 2011 The Authors. Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

Mechanisms of Enhanced Catalysis in Enzyme-DNA Nanostructures Revealed through Molecular Simulations and Experimental Analysis.

PubMed

Gao, Yingning; Roberts, Christopher C; Toop, Aaron; Chang, Chia-En A; Wheeldon, Ian

2016-08-03

Understanding and controlling the molecular interactions between enzyme substrates and DNA nanostructures has important implications in the advancement of enzyme-DNA technologies as solutions in biocatalysis. Such hybrid nanostructures can be used to create enzyme systems with enhanced catalysis by controlling the local chemical and physical environments and the spatial organization of enzymes. Here we have used molecular simulations with corresponding experiments to describe a mechanism of enhanced catalysis due to locally increased substrate concentrations. With a series of DNA nanostructures conjugated to horseradish peroxidase, we show that binding interactions between substrates and the DNA structures can increase local substrate concentrations. Increased local substrate concentrations in HRP(DNA) nanostructures resulted in 2.9- and 2.4-fold decreases in the apparent Michaelis constants of tetramethylbenzidine and 4-aminophenol, substrates of HRP with tunable binding interactions to DNA nanostructures with dissociation constants in the micromolar range. Molecular simulations and kinetic analysis also revealed that increased local substrate concentrations enhanced the rates of substrate association. Identification of the mechanism of increased local concentration of substrates in close proximity to enzymes and their active sites adds to our understanding of nanostructured biocatalysis from which we can develop guidelines for enhancing catalysis in rationally designed systems. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Trehalose Analogues: Latest Insights in Properties and Biocatalytic Production

PubMed Central

Walmagh, Maarten; Zhao, Renfei; Desmet, Tom

2015-01-01

Trehalose (α-d-glucopyranosyl α-d-glucopyranoside) is a non-reducing sugar with unique stabilizing properties due to its symmetrical, low energy structure consisting of two 1,1-anomerically bound glucose moieties. Many applications of this beneficial sugar have been reported in the novel food (nutricals), medical, pharmaceutical and cosmetic industries. Trehalose analogues, like lactotrehalose (α-d-glucopyranosyl α-d-galactopyranoside) or galactotrehalose (α-d-galactopyranosyl α-d-galactopyranoside), offer similar benefits as trehalose, but show additional features such as prebiotic or low-calorie sweetener due to their resistance against hydrolysis during digestion. Unfortunately, large-scale chemical production processes for trehalose analogues are not readily available at the moment due to the lack of efficient synthesis methods. Most of the procedures reported in literature suffer from low yields, elevated costs and are far from environmentally friendly. “Greener” alternatives found in the biocatalysis field, including galactosidases, trehalose phosphorylases and TreT-type trehalose synthases are suggested as primary candidates for trehalose analogue production instead. Significant progress has been made in the last decade to turn these into highly efficient biocatalysts and to broaden the variety of useful donor and acceptor sugars. In this review, we aim to provide an overview of the latest insights and future perspectives in trehalose analogue chemistry, applications and production pathways with emphasis on biocatalysis. PMID:26084050

Biocatalysis in organic chemistry and biotechnology: past, present, and future.

PubMed

Reetz, Manfred T

2013-08-28

Enzymes as catalysts in synthetic organic chemistry gained importance in the latter half of the 20th century, but nevertheless suffered from two major limitations. First, many enzymes were not accessible in large enough quantities for practical applications. The advent of recombinant DNA technology changed this dramatically in the late 1970s. Second, many enzymes showed a narrow substrate scope, often poor stereo- and/or regioselectivity and/or insufficient stability under operating conditions. With the development of directed evolution beginning in the 1990s and continuing to the present day, all of these problems can be addressed and generally solved. The present Perspective focuses on these and other developments which have popularized enzymes as part of the toolkit of synthetic organic chemists and biotechnologists. Included is a discussion of the scope and limitation of cascade reactions using enzyme mixtures in vitro and of metabolic engineering of pathways in cells as factories for the production of simple compounds such as biofuels and complex natural products. Future trends and problems are also highlighted, as is the discussion concerning biocatalysis versus nonbiological catalysis in synthetic organic chemistry. This Perspective does not constitute a comprehensive review, and therefore the author apologizes to those researchers whose work is not specifically treated here.

Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae.

PubMed

Zhou, Yingbiao; Zhu, Yueming; Dai, Longhai; Men, Yan; Wu, Jinhai; Zhang, Juankun; Sun, Yuanxia

2017-01-01

Melibiose is widely used as a functional carbohydrate. Whole-cell biocatalytic production of melibiose from raffinose could reduce its cost. However, characteristics of strains for whole-cell biocatalysis and mechanism of such process are unclear. We compared three different Saccharomyces cerevisiae strains (liquor, wine, and baker's yeasts) in terms of concentration variations of substrate (raffinose), target product (melibiose), and by-products (fructose and galactose) in whole-cell biocatalysis process. Distinct difference was observed in whole-cell catalytic efficiency among three strains. Furthermore, activities of key enzymes (invertase, α-galactosidase, and fructose transporter) involved in process and expression levels of their coding genes (suc2, mel1, and fsy1) were investigated. Conservation of key genes in S. cerevisiae strains was also evaluated. Results show that whole-cell catalytic efficiency of S. cerevisiae in the raffinose substrate was closely related to activity of key enzymes and expression of their coding genes. Finally, we summarized characteristics of producing strain that offered advantages, as well as contributions of key genes to excellent strains. Furthermore, we presented a dynamic mechanism model to achieve some mechanism insight for this whole-cell biocatalytic process. This pioneering study should contribute to improvement of whole-cell biocatalytic production of melibiose from raffinose.

Thermophilic archaeal enzymes and applications in biocatalysis.

PubMed

Littlechild, Jennifer A

2011-01-01

Thermophilic enzymes have advantages for their use in commercial applications and particularly for the production of chiral compounds to produce optically pure pharmaceuticals. They can be used as biocatalysts in the application of 'green chemistry'. The thermophilic archaea contain enzymes that have already been used in commercial applications such as the L-aminoacylase from Thermococcus litoralis for the resolution of amino acids and amino acid analogues. This enzyme differs from bacterial L-aminoacylases and has similarities to carboxypeptidases from other archaeal species. An amidase/γ-lactamase from Sulfolobus solfataricus has been used for the production of optically pure γ-lactam, the building block for antiviral carbocyclic nucleotides. This enzyme has similarities to the bacterial signature amidase family. An alcohol dehydrogenase from Aeropyrum pernix has been used for the production of optically pure alcohols and is related to the zinc-containing eukaryotic alcohol dehydrogenases. A transaminase and a dehalogenase from Sulfolobus species have also been studied. The archaeal transaminase is found in a pathway for serine synthesis which is found only in eukaryotes and not in bacteria. It can be used for the asymmetric synthesis of homochiral amines of high enantioselective purity. The L-2-haloacid dehalogenase has applications both in biocatalysis and in bioremediation. All of these enzymes have increased thermostability over their mesophilic counterparts.

Genome-Scale Metabolic Reconstructions and Theoretical Investigation of Methane Conversion in Methylomicrobium buryatense Strain 5G(B1)

DOE PAGES

de la Torre, Andrea; Metivier, Aisha; Chu, Frances; ...

2015-11-25

Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and are attractive systems for bio-catalysis. However, the application of natural methanotrophic strains to large-scale production of value-added chemicals/biofuels requires a number of physiological and genetic alterations. An accurate metabolic model coupled with flux balance analysis can provide a solid interpretative framework for experimental data analyses and integration.

European Congress on Biotechnology (4th) Held in Amsterdam, The Netherlands, on June 1987

DTIC Science & Technology

1988-02-19

car be used and thus higher Nmaraso Fumarate enzyme loadings can be achieved with a Glucose isomerase High- fructose corn syrup large effectiveness...is only after most of the the following reactions: glucose + oxy- glucose has been metabolized that aerobic gen + glucose oxidasa + water - gluconic...are obtained by mixing water solutions of two water -soluble * Biocatalysis polymers. Both phases have a high water * Animal cell cultures content and do

Visible light-driven NADH regeneration sensitized by proflavine for biocatalysis.

PubMed

Nam, Dong Heon; Park, Chan Beum

2012-06-18

Harvest time: Proflavine drives the reduction of NAD(+) in the presence of a Rh-based electron mediator. Photoregenerated NADH was enzymatically active for oxidation by NADH-dependent L-glutamate dehydrogenase for the synthesis of L-glutamate. This work suggests that proflavine has the potential to become an efficient light-harvesting component in biocatalytic photosynthesis driven by solar energy. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Polyethylenimine-mediated synthetic insertion of gold nanoparticles into mesoporous silica nanoparticles for drug loading and biocatalysis.

PubMed

Pandey, Prem C; Pandey, Govind; Narayan, Roger J

2017-03-27

Mesoporous silica nanoparticles (MSNPs) have been used as an efficient and safe carrier for drug delivery and biocatalysis. The surface modification of MSNPs using suitable reagents may provide a robust framework in which two or more components can be incorporated to give multifunctional capabilities (e.g., synthesis of noble metal nanoparticles within mesoporous architecture along with loading of a bioactive molecule). In this study, the authors reported on a new synthetic route for the synthesis of gold nanoparticles (AuNPs) within (1) unmodified MSNPs and (2) 3-trihydroxysilylpropyl methylphosphonate-modified MSNPs. A cationic polymer, polyethylenimine (PEI), and formaldehyde were used to mediate synthetic incorporation of AuNPs within MSNPs. The AuNPs incorporated within the mesoporous matrix were characterized by transmission electron microscopy, energy dispersive x-ray analysis, and high-resolution scanning electron microscopy. PEI in the presence of formaldehyde enabled synthetic incorporation of AuNPs in both unmodified and modified MSNPs. The use of unmodified MSNPs was associated with an increase in the polycrystalline structure of the AuNPs within the MSNPs. The AuNPs within modified MSNPs showed better catalytic activity than those within unmodified MSNPs. MSNPs with an average size of 200 nm and with a pore size of 4-6 nm were used for synthetic insertion of AuNPs. It was found that the PEI coating enabled AuNPs synthesis within the mesopores in the presence of formaldehyde or tetrahydrofuran hydroperoxide at a temperature between 10 and 25 °C or at 60 °C in the absence of organic reducing agents. The as-made AuNP-inserted MSNPs exhibited enhanced catalytic activity. For example, these materials enabled rapid catalytic oxidation of the o-dianisidine substrate to produce a colored solution in proportion to the amount of H 2 O 2 generated as a function of glucose oxidase-catalyzed oxidation of glucose; a linear concentration range from 80 to 800 μM and a detection limit as low as 80 μM were observed. The mesoscale pores of the as developed AuNP-inserted MSNPs were also used to entrap the hydrophobic drug paclitaxel. The results of this study indicate the potential use of the AuNP-inserted MSNPs in biocatalysis and drug delivery.

The use of ene adducts to study and engineer enoyl-thioester reductases.

PubMed

Rosenthal, Raoul G; Vögeli, Bastian; Quade, Nick; Capitani, Guido; Kiefer, Patrick; Vorholt, Julia A; Ebert, Marc-Olivier; Erb, Tobias J

2015-06-01

An improved understanding of enzymes' catalytic proficiency and stereoselectivity would further enable applications in chemistry, biocatalysis and industrial biotechnology. We use a chemical probe to dissect individual catalytic steps of enoyl-thioester reductases (Etrs), validating an active site tyrosine as the cryptic proton donor and explaining how it had eluded definitive identification. This information enabled the rational redesign of Etr, yielding mutants that create products with inverted stereochemistry at wild type-like turnover frequency.

Virtual special issue on catalysis at the U.S. Department of Energy's National Laboratories

DOE PAGES

Pruski, Marek; Sadow, Aaron D.; Slowing, Igor I.; ...

2016-04-21

Here the catalysis research at the U.S. Department of Energy's (DOE's) National Laboratories covers a wide range of research topics in heterogeneous catalysis, homogeneous/molecular catalysis, biocatalysis, electrocatalysis, and surface science. Since much of the work at National Laboratories is funded by DOE, the research is largely focused on addressing DOE's mission to ensure America's security and prosperity by addressing its energy, environmental, and nuclear challenges through transformative science and technology solutions.

Cold and Hot Extremozymes: Industrial Relevance and Current Trends

PubMed Central

Sarmiento, Felipe; Peralta, Rocío; Blamey, Jenny M.

2015-01-01

The development of enzymes for industrial applications relies heavily on the use of microorganisms. The intrinsic properties of microbial enzymes, e.g., consistency, reproducibility, and high yields along with many others, have pushed their introduction into a wide range of products and industrial processes. Extremophilic microorganisms represent an underutilized and innovative source of novel enzymes. These microorganisms have developed unique mechanisms and molecular means to cope with extreme temperatures, acidic and basic pH, high salinity, high radiation, low water activity, and high metal concentrations among other environmental conditions. Extremophile-derived enzymes, or extremozymes, are able to catalyze chemical reactions under harsh conditions, like those found in industrial processes, which were previously not thought to be conducive for enzymatic activity. Due to their optimal activity and stability under extreme conditions, extremozymes offer new catalytic alternatives for current industrial applications. These extremozymes also represent the cornerstone for the development of environmentally friendly, efficient, and sustainable industrial technologies. Many advances in industrial biocatalysis have been achieved in recent years; however, the potential of biocatalysis through the use of extremozymes is far from being fully realized. In this article, the adaptations and significance of psychrophilic, thermophilic, and hyperthermophilic enzymes, and their applications in selected industrial markets will be reviewed. Also, the current challenges in the development and mass production of extremozymes as well as future prospects and trends for their biotechnological application will be discussed. PMID:26539430

Bioconversion of methane to lactate by an obligate methanotrophic bacterium

DOE PAGES

Henard, Calvin A.; Smith, Holly; Dowe, Nancy; ...

2016-02-23

Methane is the second most abundant greenhouse gas (GHG), with nearly 60% of emissions derived from anthropogenic sources. Microbial conversion of methane to fuels and value-added chemicals offers a means to reduce GHG emissions, while also valorizing this otherwise squandered high-volume, high-energy gas. However, to date, advances in methane biocatalysis have been constrained by the low-productivity and limited genetic tractability of natural methane-consuming microbes. Here, leveraging recent identification of a novel, tractable methanotrophic bacterium, Methylomicrobium buryatense, we demonstrate microbial biocatalysis of methane to lactate, an industrial platform chemical. Heterologous overexpression of a Lactobacillus helveticus L-lactate dehydrogenase in M. buryatense resultedmore » in an initial titer of 0.06 g lactate/L from methane. Cultivation in a 5 L continuously stirred tank bioreactor enabled production of 0.8 g lactate/L, representing a 13-fold improvement compared to the initial titer. The yields (0.05 g lactate/g methane) and productivity (0.008 g lactate/L/h) indicate the need and opportunity for future strain improvement. Additionally, real-time analysis of methane utilization implicated gas-to-liquid transfer and/or microbial methane consumption as process limitations. This work opens the door to develop an array of methanotrophic bacterial strain-engineering strategies currently employed for biocatalytic sugar upgrading to “green” chemicals and fuels.« less

Bioconversion of methane to lactate by an obligate methanotrophic bacterium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henard, Calvin A.; Smith, Holly; Dowe, Nancy

Methane is the second most abundant greenhouse gas (GHG), with nearly 60% of emissions derived from anthropogenic sources. Microbial conversion of methane to fuels and value-added chemicals offers a means to reduce GHG emissions, while also valorizing this otherwise squandered high-volume, high-energy gas. However, to date, advances in methane biocatalysis have been constrained by the low-productivity and limited genetic tractability of natural methane-consuming microbes. Here, leveraging recent identification of a novel, tractable methanotrophic bacterium, Methylomicrobium buryatense, we demonstrate microbial biocatalysis of methane to lactate, an industrial platform chemical. Heterologous overexpression of a Lactobacillus helveticus L-lactate dehydrogenase in M. buryatense resultedmore » in an initial titer of 0.06 g lactate/L from methane. Cultivation in a 5 L continuously stirred tank bioreactor enabled production of 0.8 g lactate/L, representing a 13-fold improvement compared to the initial titer. The yields (0.05 g lactate/g methane) and productivity (0.008 g lactate/L/h) indicate the need and opportunity for future strain improvement. Additionally, real-time analysis of methane utilization implicated gas-to-liquid transfer and/or microbial methane consumption as process limitations. This work opens the door to develop an array of methanotrophic bacterial strain-engineering strategies currently employed for biocatalytic sugar upgrading to “green” chemicals and fuels.« less

Bioconversion of methane to lactate by an obligate methanotrophic bacterium

PubMed Central

Henard, Calvin A.; Smith, Holly; Dowe, Nancy; Kalyuzhnaya, Marina G.; Pienkos, Philip T.; Guarnieri, Michael T.

2016-01-01

Methane is the second most abundant greenhouse gas (GHG), with nearly 60% of emissions derived from anthropogenic sources. Microbial conversion of methane to fuels and value-added chemicals offers a means to reduce GHG emissions, while also valorizing this otherwise squandered high-volume, high-energy gas. However, to date, advances in methane biocatalysis have been constrained by the low-productivity and limited genetic tractability of natural methane-consuming microbes. Here, leveraging recent identification of a novel, tractable methanotrophic bacterium, Methylomicrobium buryatense, we demonstrate microbial biocatalysis of methane to lactate, an industrial platform chemical. Heterologous overexpression of a Lactobacillus helveticus L-lactate dehydrogenase in M. buryatense resulted in an initial titer of 0.06 g lactate/L from methane. Cultivation in a 5 L continuously stirred tank bioreactor enabled production of 0.8 g lactate/L, representing a 13-fold improvement compared to the initial titer. The yields (0.05 g lactate/g methane) and productivity (0.008 g lactate/L/h) indicate the need and opportunity for future strain improvement. Additionally, real-time analysis of methane utilization implicated gas-to-liquid transfer and/or microbial methane consumption as process limitations. This work opens the door to develop an array of methanotrophic bacterial strain-engineering strategies currently employed for biocatalytic sugar upgrading to “green” chemicals and fuels. PMID:26902345

[Biotechnological potential of methylotrophic bacteria: a review of current status and future prospects].

PubMed

Trotsenko, Iu A; Doronina, N V; Khmelenina, V N

2005-01-01

Major results of the authors' findings on the implementation of biotechnological potential of aerobic methylobacteria and methanotrophs for obtaining forage proteins, biopolymers (polybutyrate and polysaccharides), enzymes (oxidoreductases), and bioprotectors (ectoin), as well as for degrading toxic C1 and Cn compounds have been reviewed. Unique features of the structural and functional organization of the metabolism of extremophilic (tolerant) methylotrophs are discussed, with a view for their prospective use in various fields of modern biotechnology, including biocatalysis and nanotechnology.

Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2′-deoxyadenosine substrates†

PubMed Central

Cobb, Steven L.; Deng, Hai; McEwan, Andrew R.; Naismith, James H.; O’Hagan, David; Robinson, David A.

2012-01-01

The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C–F bond. We now report that the enzyme will accept 2′-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C–F bond formation. PMID:16604208

Discovery of a diazo-forming enzyme in cremeomycin biosynthesis.

PubMed

Waldman, Abraham J; Balskus, Emily P

2018-05-17

The molecular architectures and potent bioactivities of diazo-containing natural products have attracted the interest of synthetic and biological chemists. Despite this attention, the biosynthetic enzymes involved in diazo group construction have not been identified. Here, we show the ATP-dependent enzyme CreM installs the diazo group in cremeomycin via late-stage N-N bond formation using nitrite. This finding should inspire efforts to use diazo-forming enzymes in biocatalysis and synthetic biology and enable genome-based discovery of new diazo-containing metabolites.

Process boundaries of irreversible scCO2 -assisted phase separation in biphasic whole-cell biocatalysis.

PubMed

Brandenbusch, Christoph; Glonke, Sebastian; Collins, Jonathan; Hoffrogge, Raimund; Grunwald, Klaudia; Bühler, Bruno; Schmid, Andreas; Sadowski, Gabriele

2015-11-01

The formation of stable emulsions in biphasic biotransformations catalyzed by microbial cells turned out to be a major hurdle for industrial implementation. Recently, a cost-effective and efficient downstream processing approach, using supercritical carbon dioxide (scCO2 ) for both irreversible emulsion destabilization (enabling complete phase separation within minutes of emulsion treatment) and product purification via extraction has been proposed by Brandenbusch et al. (2010). One of the key factors for a further development and scale-up of the approach is the understanding of the mechanism underlying scCO2 -assisted phase separation. A systematic approach was applied within this work to investigate the various factors influencing phase separation during scCO2 treatment (that is pressure, exposure of the cells to CO2 , and changes of cell surface properties). It was shown that cell toxification and cell disrupture are not responsible for emulsion destabilization. Proteins from the aqueous phase partially adsorb to cells present at the aqueous-organic interface, causing hydrophobic cell surface characteristics, and thus contribute to emulsion stabilization. By investigating the change in cell-surface hydrophobicity of these cells during CO2 treatment, it was found that a combination of catastrophic phase inversion and desorption of proteins from the cell surface is responsible for irreversible scCO2 mediated phase separation. These findings are essential for the definition of process windows for scCO2 -assisted phase separation in biphasic whole-cell biocatalysis. © 2015 Wiley Periodicals, Inc.

From water-in-oil to oil-in-water emulsions to optimize the production of fatty acids using ionic liquids in micellar systems.

PubMed

Santos, Luísa D F; Coutinho, João A P; Ventura, Sónia P M

2015-01-01

Biocatalysis is nowadays considered as one of the most important tools in green chemistry. The elimination of multiple steps involved in some of the most complex chemical synthesis, reducing the amounts of wastes and hazards, thus increasing the reaction yields and decreasing the intrinsic costs, are the major advantages of biocatalysis. This work aims at improving the enzymatic hydrolysis of olive oil to produce valuable fatty acids through emulsion systems formed by long alkyl chain ionic liquids (ILs). The optimization of the emulsion and the best conditions to maximize the production of fatty acids were investigated. The stability of the emulsion was characterized considering the effect of several parameters, namely, the IL and its concentration and different water/olive oil volumetric ratios. ILs from the imidazolium and phosphonium families were evaluated. The results suggest that the ILs effect on the hydrolysis performance varies with the water concentration and the emulsion system formed, that is, water-in-oil or oil-in-water emulsion. Although at low water concentrations, the presence of ILs does not present any advantages for the hydrolysis reaction, at high water contents (in oil-in-water emulsions), the imidazolium-based IL acts as an enhancer of the lipase catalytic capacity, super-activating 1.8 times the enzyme, and consequently promoting the complete hydrolysis of the olive oil for the highest water contents [85% (v/v)]. © 2015 American Institute of Chemical Engineers.

Biocatalysis and biomimetics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Burrington, J.D.; Clark, D.S.

1989-01-01

This book presents recent advances in catalytic science and biotechnology. The chapters illustrate how many of the key challenges in biotechnology can be addressed by bringing together traditionally separate disciplines within chemistry and biology. The authors focus on emerging enabling technologies at the interfaces of catalysis and biology that will provide new opportunities for the chemicals industries. Key aspects to be presented within this major theme of catalysis and biotechnology are biomimetics and hybrid catalysts, biocatalytic applications of computers and expert systems, enzyme solid-state structure and immobilization, enzyme structure-activity relationships, and the use of enzymes under novel conditions.

Developments in biotechnological research in Austria

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kubicek, C.P.

1996-12-01

Austria is a small European country with a small number of universities and biotechnological industries, but with great efforts in the implementation of environmental consciousness and corresponding legal standards. This review attempts to describe the biotechnological landscape of Austria, thereby focusing on the highlights in research by industry, universities, and research laboratories, as published during 1990 to early 1995. These will include microbial metabolite (organic acids, antibiotics) and biopolymer (polyhydroxibutyrate, S-layers) production; enzyme (cellulases, hemicellulases, ligninases) technology and biocatalysis; environmental biotechnology; plant breeding and plant protection; mammalian cell products; fermenter design; and bioprocess engineering. 234 refs.

Recent developments in biocatalysis in multiphasic ionic liquid reaction systems.

PubMed

Meyer, Lars-Erik; von Langermann, Jan; Kragl, Udo

2018-06-01

Ionic liquids are well known and frequently used 'designer solvents' for biocatalytic reactions. This review highlights recent achievements in the field of multiphasic ionic liquid-based reaction concepts. It covers classical biphasic systems including supported ionic liquid phases, thermo-regulated multi-component solvent systems (TMS) and polymerized ionic liquids. These powerful concepts combine unique reaction conditions with a high potential for future applications on a laboratory and industrial scale. The presence of a multiphasic system simplifies downstream processing due to the distribution of the catalyst and reactants in different phases.

Biocatalytic transformations in ionic liquids.

PubMed

van Rantwijk, Fred; Madeira Lau, Rute; Sheldon, Roger A

2003-03-01

Room temperature ionic liquids are non-volatile, thermally stable and highly polar; they are also moderately hydrophilic solvents. Here, we discuss their use as reaction media for biocatalysis. Enzymes of widely diverging types are catalytically active in ionic liquids or aqueous biphasic ionic liquid systems. Lipases, in particular, maintain their activity in anhydrous ionic liquid media; the (enantio)selectivity and operational stability are often better than in traditional media. The unconventional solvent properties of ionic liquids have been exploited in biocatalyst recycling and product recovery schemes that are not feasible with traditional solvent systems.

The growing impact of lyophilized cell-free protein expression systems

PubMed Central

Hunt, J. Porter; Yang, Seung Ook; Wilding, Kristen M.; Bundy, Bradley C.

2017-01-01

ABSTRACT Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis. PMID:27791452

Recents patents in the use of peroxidases.

PubMed

Alvarado, Berenize; Torres, Eduardo

2009-01-01

Peroxidases are hemoenzymes with a wide range of applications, from fine chemical synthesis to environmental biocatalysis. These outstanding biocatalysts are able to catalyze reactions such as heteroatom oxidation (N- and S-oxidation), epoxidation, hydroxylation, and the oxidation of alcohols and indole, often giving high yields and enantiomeric excess values. This makes these biocatalysts very useful for application to several biotechnological processes. In this paper, recent advances and patents surrounding the use of peroxidases are reviewed, covering different aspects related to the applications of peroxidases and the modifications carried out to improve their functionality as biocatalysts.

Rationally engineering natural protein assemblies in nanobiotechnology.

PubMed

Howorka, Stefan

2011-08-01

Multimeric protein assemblies are essential components in viruses, bacteria, eukaryotic cells, and organisms where they act as cytoskeletal scaffold, storage containers, or for directional transport. The bottom-up structures can be exploited in nanobiotechnology by harnessing their built-in properties and combining them with new functional modules. This review summarizes the design principles of natural protein assemblies, highlights recent progress in their structural elucidation, and shows how rational engineering can create new biomaterials for applications in vaccine development, biocatalysis, materials science, and synthetic biology. Copyright © 2011 Elsevier Ltd. All rights reserved.

Enzyme cascades activated on topologically programmed DNA scaffolds

NASA Astrophysics Data System (ADS)

Wilner, Ofer I.; Weizmann, Yossi; Gill, Ron; Lioubashevski, Oleg; Freeman, Ronit; Willner, Itamar

2009-04-01

The ability of DNA to self-assemble into one-, two- and three-dimensional nanostructures, combined with the precision that is now possible when positioning nanoparticles or proteins on DNA scaffolds, provide a promising approach for the self-organization of composite nanostructures. Predicting and controlling the functions that emerge in self-organized biomolecular nanostructures is a major challenge in systems biology, and although a number of innovative examples have been reported, the emergent properties of systems in which enzymes are coupled together have not been fully explored. Here, we report the self-assembly of a DNA scaffold made of DNA strips that include `hinges' to which biomolecules can be tethered. We attach either two enzymes or a cofactor-enzyme pair to the scaffold, and show that enzyme cascades or cofactor-mediated biocatalysis can proceed effectively; similar processes are not observed in diffusion-controlled homogeneous mixtures of the same components. Furthermore, because the relative position of the two enzymes or the cofactor-enzyme pair is determined by the topology of the DNA scaffold, it is possible to control the reactivity of the system through the design of the individual DNA strips. This method could lead to the self-organization of complex multi-enzyme cascades.

Magnetic biocatalysts and their uses to obtain biodiesel and biosurfactants

PubMed Central

López, Carmen; Cruz-Izquierdo, Álvaro; Picó, Enrique A.; García-Bárcena, Teresa; Villarroel, Noelia; Llama, María J.; Serra, Juan L.

2014-01-01

Nanobiocatalysis, as the synergistic combination of nanotechnology and biocatalysis, is rapidly emerging as a new frontier of biotechnology. The use of immobilized enzymes in industrial applications often presents advantages over their soluble counterparts, mainly in view of stability, reusability and simpler operational processing. Because of their singular properties, such as biocompatibility, large and modifiable surface and easy recovery, iron oxide magnetic nanoparticles (MNPs) are attractive super-paramagnetic materials that serve as a support for enzyme immobilization and facilitate separations by applying an external magnetic field. Cross-linked enzyme aggregates (CLEAs) have several benefits in the context of industrial applications since they can be cheaply and easily prepared from unpurified enzyme extracts and show improved storage and operational stability against denaturation by heat and organic solvents. In this work, by using the aforementioned advantages of MNPs of magnetite and CLEAs, we prepared two robust magnetically-separable types of nanobiocatalysts by binding either soluble enzyme onto the surface of MNPs functionalized with amino groups or by cross-linking aggregates of enzyme among them and to MNPs to obtain magnetic CLEAs. For this purpose the lipase B of Candida antarctica (CALB) was used. The hydrolytic and biosynthetic activities of the resulting magnetic nanobiocatalysts were assessed in aqueous and organic media. Thus, the hydrolysis of triglycerides and the transesterification reactions to synthesize biodiesel and biosurfactants were studied using magnetic CLEAs of CALB. The efficiency and easy performance of this magnetic biocatalysis validates this proof of concept and sets the basis for the application of magnetic CLEAs at industrial scale. PMID:25207271

Enzyme and microbial technology for synthesis of bioactive oligosaccharides: an update.

PubMed

Chen, Rachel

2018-04-01

Oligosaccharides, in either free or bound forms, play crucial roles in a wide range of biological processes. Increasing appreciation of their roles in cellular communication, interaction, pathogenesis, and prebiotic functions has stimulated tremendous interests in their synthesis. Pure and structurally defined oligosaccharides are essential for fundamental studies. On the other hand, for those with near term medical and nutraceutical applications, their large-scale synthesis is necessary. Unfortunately, oligosaccharides are notoriously difficult in their synthesis, and their enormous diverse structures leave a vast gap between what have been synthesized in laboratory and those present in various biological systems. While enzymes and microbes are nature's catalysts for oligosaccharides, their effective use is not without challenges. Using examples of galactose-containing oligosaccharides, this review analyzes the pros and cons of these two forms of biocatalysts and provides an updated view on the status of biocatalysis in this important field. Over the past few years, a large number of novel galactosidases were discovered and/or engineered for improved synthesis via transglycosylation. The use of salvage pathway for regeneration of uridine diphosphate (UDP)-galactose has made the use of Leloir glycosyltransferases simpler and more efficient. The recent success of large-scale synthesis of 2' fucosyllactose heralded the power of whole-cell biocatalysis as a scalable technology. While it still lags behind enzyme catalysis in terms of the number of oligosaccharides synthesized, an acceleration in the use of this form of biocatalyst is expected as rapid advances in synthetic biology have made the engineering of whole cell biocatalysts less arduous and less time consuming.

Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l‐erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase

PubMed Central

Wei, Yu‐Chia; Braun‐Galleani, Stephanie; Henríquez, Maria José; Bandara, Sahan

2017-01-01

Transketolase is a proven biocatalytic tool for asymmetric carbon‐carbon bond formation, both as a purified enzyme and within bacterial whole‐cell biocatalysts. The performance of Pichia pastoris as a host for transketolase whole‐cell biocatalysis was investigated using a transketolase‐overexpressing strain to catalyze formation of l‐erythrulose from β‐hydroxypyruvic acid and glycolaldehyde substrates. Pichia pastoris transketolase coding sequence from the locus PAS_chr1‐4_0150 was subcloned downstream of the methanol‐inducible AOX1 promoter in a plasmid for transformation of strain GS115, generating strain TK150. Whole and disrupted TK150 cells from shake flasks achieved 62% and 65% conversion, respectively, under optimal pH and methanol induction conditions. In a 300 μL reaction, TK150 samples from a 1L fed‐batch fermentation achieved a maximum l‐erythrulose space time yield (STY) of 46.58 g L−1 h−1, specific activity of 155 U gCDW−1, product yield on substrate (Yp/s) of 0.52 mol mol−1 and product yield on catalyst (Yp/x) of 2.23g gCDW−1. We have successfully exploited the rapid growth and high biomass characteristics of Pichia pastoris in whole cell biocatalysis. At high cell density, the engineered TK150 Pichia pastoris strain tolerated high concentrations of substrate and product to achieve high STY of the chiral sugar l‐erythrulose. © 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 34:99–106, 2018 PMID:29086489

Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

NASA Astrophysics Data System (ADS)

Viefhues, Martina; Sun, Shiwen; Valikhani, Donya; Nidetzky, Bernd; Vrouwe, Elwin X.; Mayr, Torsten; Bolivar, Juan M.

2017-06-01

Flow microreactors utilizing immobilized enzymes are of great interest in biocatalysis development. Most of the common devices are permanently closed, single-use systems, which allow limited physical and chemical surface modifications and evaluation methods. In this paper we will present resealable flowcells that overcome these limitations and moreover allow a quick and easy integration of sensor systems, because of the use of modular building blocks. The devices were utilized to study the enzyme activity of glucose oxidase immobilized on chemically modified glass surfaces under flow conditions, employing integrated optical oxygen sensors for on-line monitoring.

Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.

PubMed

Schrewe, Manfred; Julsing, Mattijs K; Lange, Kerstin; Czarnotta, Eik; Schmid, Andreas; Bühler, Bruno

2014-09-01

The oxyfunctionalization of unactivated C−H bonds can selectively and efficiently be catalyzed by oxygenase-containing whole-cell biocatalysts. Recombinant Escherichia coli W3110 containing the alkane monooxygenase AlkBGT and the outer membrane protein AlkL from Pseudomonas putida GPo1 have been shown to efficiently catalyze the terminal oxyfunctionalization of renewable fatty acid methyl esters yielding bifunctional products of interest for polymer synthesis. In this study, AlkBGTL-containing E. coli W3110 is shown to catalyze the multistep conversion of dodecanoic acid methyl ester (DAME) via terminal alcohol and aldehyde to the acid, exhibiting Michaelis-Menten-type kinetics for each reaction step. In two-liquid phase biotransformations, the product formation pattern was found to be controlled by DAME availability. Supplying DAME as bulk organic phase led to accumulation of the terminal alcohol as the predominant product. Limiting DAME availability via application of bis(2-ethylhexyl)phthalate (BEHP) as organic carrier solvent enabled almost exclusive acid accumulation. Furthermore, utilization of BEHP enhanced catalyst stability by reducing toxic effects of substrate and products. A further shift towards the overoxidized products was achieved by co-expression of the gene encoding the alcohol dehydrogenase AlkJ, which was shown to catalyze efficient and irreversible alcohol to aldehyde oxidation in vivo. With DAME as organic phase, the aldehyde accumulated as main product using resting cells containing AlkBGT, AlkL, as well as AlkJ. This study highlights the versatility of whole-cell biocatalysis for synthesis of industrially relevant bifunctional building blocks and demonstrates how integrated reaction and catalyst engineering can be implemented to control product formation patterns in biocatalytic multistep reactions. © 2014 Wiley Periodicals, Inc.

Microgel coating of magnetic nanoparticles via bienzyme-mediated free-radical polymerization for colorimetric detection of glucose

NASA Astrophysics Data System (ADS)

Wu, Qing; Wang, Xia; Liao, Chuanan; Wei, Qingcong; Wang, Qigang

2015-10-01

This study describes a new strategy for the fabrication of magnetic core-shell microgels by free-radical polymerization triggered by the cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP). The mild polymerization around the interface of the magnetic nanoparticles permits the mild coating of the microgel layer with excellent characteristics for various applications in biocatalysis and medical diagnostics, as well as in clinical fields. The immobilized bienzyme within the microgel has a largely retained activity relative to the non-immobilized one. The confining effect of the microgel and the well designed distance between the two enzymes can benefit the diffusion of intermediates to the HRP active site. The final microgels can be incontestably employed as sensitive biosensors for colorimetric glucose detection.This study describes a new strategy for the fabrication of magnetic core-shell microgels by free-radical polymerization triggered by the cascade reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP). The mild polymerization around the interface of the magnetic nanoparticles permits the mild coating of the microgel layer with excellent characteristics for various applications in biocatalysis and medical diagnostics, as well as in clinical fields. The immobilized bienzyme within the microgel has a largely retained activity relative to the non-immobilized one. The confining effect of the microgel and the well designed distance between the two enzymes can benefit the diffusion of intermediates to the HRP active site. The final microgels can be incontestably employed as sensitive biosensors for colorimetric glucose detection. Electronic supplementary information (ESI) available: Experimental details and ESI figures. See DOI: 10.1039/c5nr05716g

Nanobio interfaces: charge control of enzyme/inorganic interfaces for advanced biocatalysis.

PubMed

Deshapriya, Inoka K; Kumar, Challa V

2013-11-19

Specific approaches to the rational design of nanobio interfaces for enzyme and protein binding to nanomaterials are vital for engineering advanced, functional nanobiomaterials for biocatalysis, sensing, and biomedical applications. This feature article presents an overview of our recent discoveries on structural, functional, and mechanistic details of how enzymes interact with inorganic nanomaterials and how they can be controlled in a systematic manner using α-Zr(IV)phosphate (α-ZrP) as a model system. The interactions of a number of enzymes having a wide array of surface charges, sizes, and functional groups are investigated. Interactions are carefully controlled to screen unfavorable repulsions and enhance favorable interactions for high affinity, structure retention, and activity preservation. In specific cases, catalytic activities and substrate selectivities are improved over those of the pristine enzymes, and two examples of high activity near the boiling point of water have been demonstrated. Isothermal titration calorimetric studies indicated that enzyme binding is coupled to ion sequestration or release to or from the nanobio interface, and binding is controlled in a rational manner. We learned that (1) bound enzyme stabilities are improved by lowering the entropy of the denatured state; (2) maximal loadings are obtained by matching charge footprints of the enzyme and the nanomaterial surface; (3) binding affinities are improved by ion sequestration at the nanobio interface; and (4) maximal enzyme structure retention is obtained by biophilizing the nanobio interface with protein glues. The chemical and physical manipulations of the nanobio interface are significant not only for understanding the complex behaviors of enzymes at biological interfaces but also for desiging better functional nanobiomaterials for a wide variety of practical applications.

Biocatalysis of a Paclitaxel Analogue: Conversion of Baccatin III to N-Debenzoyl-N-(2-furoyl)paclitaxel and Characterization of an Amino Phenylpropanoyl CoA Transferase.

PubMed

Thornburg, Chelsea K; Walter, Tyler; Walker, Kevin D

2017-11-07

In this study, we demonstrate an enzyme cascade reaction using a benzoate CoA ligase (BadA), a modified nonribosomal peptide synthase (PheAT), a phenylpropanoyltransferase (BAPT), and a benzoyltransferase (NDTNBT) to produce an anticancer paclitaxel analogue and its precursor from the commercially available biosynthetic intermediate baccatin III. BAPT and NDTNBT are acyltransferases on the biosynthetic pathway to the antineoplastic drug paclitaxel in Taxus plants. For this study, we addressed the recalcitrant expression of BAPT by expressing it as a soluble maltose binding protein fusion (MBP-BAPT). Further, the preparative-scale in vitro biocatalysis of phenylisoserinyl CoA using PheAT enabled thorough kinetic analysis of MBP-BAPT, for the first time, with the cosubstrate baccatin III. The turnover rate of MBP-BAPT was calculated for the product N-debenzoylpaclitaxel, a key intermediate to various bioactive paclitaxel analogues. MBP-BAPT also converted, albeit more slowly, 10-deacetylbaccatin III to N-deacyldocetaxel, a precursor of the pharmaceutical docetaxel. With PheAT available to make phenylisoserinyl CoA and kinetic characterization of MBP-BAPT, we used Michaelis-Menten parameters of the four enzymes to adjust catalyst and substrate loads in a 200-μL one-pot reaction. This multienzyme network produced a paclitaxel analogue N-debenzoyl-N-(2-furoyl)paclitaxel (230 ng) that is more cytotoxic than paclitaxel against certain macrophage cell types. Also in this pilot reaction, the versatile N-debenzoylpaclitaxel intermediate was made at an amount 20-fold greater than the N-(2-furoyl) product. This reaction network has great potential for optimization to scale-up production and is attractive in its regioselective O- and N-acylation steps that remove protecting group manipulations used in paclitaxel analogue synthesis.

The CoFactor database: organic cofactors in enzyme catalysis.

PubMed

Fischer, Julia D; Holliday, Gemma L; Thornton, Janet M

2010-10-01

Organic enzyme cofactors are involved in many enzyme reactions. Therefore, the analysis of cofactors is crucial to gain a better understanding of enzyme catalysis. To aid this, we have created the CoFactor database. CoFactor provides a web interface to access hand-curated data extracted from the literature on organic enzyme cofactors in biocatalysis, as well as automatically collected information. CoFactor includes information on the conformational and solvent accessibility variation of the enzyme-bound cofactors, as well as mechanistic and structural information about the hosting enzymes. The database is publicly available and can be accessed at http://www.ebi.ac.uk/thornton-srv/databases/CoFactor.

Protein crystallography and site-direct mutagenesis analysis of the poly(ethylene terephthalate) hydrolase PETase from Ideonella sakaiensis.

PubMed

Liu, Bing; He, Lihui; Wang, Liping; Li, Tao; Li, Changcheng; Liu, Huayi; Luo, Yunzi; Bao, Rui

2018-03-30

Compared with traditional recycle strategies, biodegradation provides a sustainable solution for poly (ethylene terephthalate) (PET) wastes disposal. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET, which provides a prominent prospect on PET degradation. Based on the biochemical analysis, we propose that the wide substrate-binding pocket is critical for its excellent property on crystallized PET hydrolysis. Structure-guided site-directed mutagenesis exhibited improvement in PETase catalytic efficiency, providing valuable insight on how the molecular engineering of PETase can optimize its application in biocatalysis. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs).

PubMed

Sheldon, Roger A

2011-11-01

Cross-linked enzyme aggregates (CLEAs) have many economic and environmental benefits in the context of industrial biocatalysis. They are easily prepared from crude enzyme extracts, and the costs of (often expensive) carriers are circumvented. They generally exhibit improved storage and operational stability towards denaturation by heat, organic solvents, and autoproteolysis and are stable towards leaching in aqueous media. Furthermore, they have high catalyst productivities (kilograms product per kilogram biocatalyst) and are easy to recover and recycle. Yet another advantage derives from the possibility to co-immobilize two or more enzymes to provide CLEAs that are capable of catalyzing multiple biotransformations, independently or in sequence as catalytic cascade processes.

Network design and analysis for multi-enzyme biocatalysis.

PubMed

Blaß, Lisa Katharina; Weyler, Christian; Heinzle, Elmar

2017-08-10

As more and more biological reaction data become available, the full exploration of the enzymatic potential for the synthesis of valuable products opens up exciting new opportunities but is becoming increasingly complex. The manual design of multi-step biosynthesis routes involving enzymes from different organisms is very challenging. To harness the full enzymatic potential, we developed a computational tool for the directed design of biosynthetic production pathways for multi-step catalysis with in vitro enzyme cascades, cell hydrolysates and permeabilized cells. We present a method which encompasses the reconstruction of a genome-scale pan-organism metabolic network, path-finding and the ranking of the resulting pathway candidates for proposing suitable synthesis pathways. The network is based on reaction and reaction pair data from the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the thermodynamics calculator eQuilibrator. The pan-organism network is especially useful for finding the most suitable pathway to a target metabolite from a thermodynamic or economic standpoint. However, our method can be used with any network reconstruction, e.g. for a specific organism. We implemented a path-finding algorithm based on a mixed-integer linear program (MILP) which takes into account both topology and stoichiometry of the underlying network. Unlike other methods we do not specify a single starting metabolite, but our algorithm searches for pathways starting from arbitrary start metabolites to a target product of interest. Using a set of biochemical ranking criteria including pathway length, thermodynamics and other biological characteristics such as number of heterologous enzymes or cofactor requirement, it is possible to obtain well-designed meaningful pathway alternatives. In addition, a thermodynamic profile, the overall reactant balance and potential side reactions as well as an SBML file for visualization are generated for each pathway alternative. We present an in silico tool for the design of multi-enzyme biosynthetic production pathways starting from a pan-organism network. The method is highly customizable and each module can be adapted to the focus of the project at hand. This method is directly applicable for (i) in vitro enzyme cascades, (ii) cell hydrolysates and (iii) permeabilized cells.

Genomics of Clostridium

NASA Astrophysics Data System (ADS)

Jacobson, Mark Joseph; Johnson, Eric A.

The clostridia have a rich history and contemporary importance in industrial, environmental, and medical microbiology. Due to their ability to form endospores, clostridia are ubiquitous in nature and are found in many environments, especially in soils and the intestinal tract of animals including humans. Many clostridia cause devastating diseases of humans and animals, such as botulism, tetanus, and gas gangrene, through the production of protein toxins. The clostridia produce more protein toxins that are lethal for humans and animals than any other bacterial genus (Johnson, 2005; Van Heyningen, 1950). Other species are important in the formation of solvents and organic acids by anaerobic fermentations or as a source of unique enzymes for biocatalysis (Bradshaw and Johnson, 2010; Hatheway and Johnson, 1998).

White biotechnology: ready to partner and invest in.

PubMed

Kircher, Manfred

2006-01-01

It needs three factors to build an industry: market demand, product vision and capital. White biotechnology already produces high volume products such as feed additive amino acids and specialty products like enzymes for enantioselective biocatalysis. It serves large and diverse markets in the nutrition, wellness, pharmaceutical, agricultural and chemical industry. The total volume adds up to $ 50 billion worldwide. In spite of its proven track record, white biotechnology so far did not attract as much capital as red and even green biotechnology. However, the latest finance indicators confirm the continuously growing attractiveness of investment opportunities in white biotechnology. This article discusses white biotechnology's position and potential in the finance market and success factors.

Beyond relationships between homogeneous and heterogeneous catalysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dixon, David A.; Katz, Alexander; Arslan, Ilke

2014-08-13

Scientists who regard catalysis as a coherent field have been striving for decades to articulate the fundamental unifying principles. But because these principles seem to be broader than chemistry, chemical engineering, and materials science combined, catalytic scientists commonly interact within the sub-domains of homogeneous, heterogeneous, and bio-catalysis, and increasingly within even narrower domains such as organocatalysis, phase-transfer catalysis, acid-base catalysis, zeolite catalysis, etc. Attempts to unify catalysis have motivated researchers to find relationships between homogeneous and heterogeneous catalysis and to mimic enzymes. These themes have inspired vibrant international meetings and workshops, and we have benefited from the idea exchanges andmore » have some thoughts about a path forward.« less

Nitrilases in nitrile biocatalysis: recent progress and forthcoming research

PubMed Central

2012-01-01

Over the past decades, nitrilases have drawn considerable attention because of their application in nitrile degradation as prominent biocatalysts. Nitrilases are derived from bacteria, filamentous fungi, yeasts, and plants. In-depth investigations on their natural sources function mechanisms, enzyme structure, screening pathways, and biocatalytic properties have been conducted. Moreover, the immobilization, purification, gene cloning and modifications of nitrilase have been dwelt upon. Some nitrilases are used commercially as biofactories for carboxylic acids production, waste treatment, and surface modification. This critical review summarizes the current status of nitrilase research, and discusses a number of challenges and significant attempts in its further development. Nitrilase is a significant and promising biocatalyst for catalytic applications. PMID:23106943

Biocatalysis: applications and potentials for the chemical industry.

PubMed

Thomas, Stuart M; DiCosimo, Robert; Nagarajan, Vasantha

2002-06-01

The chemical industry is exploring the use of renewable feed stocks to improve sustainability, prompting the exploration of bioprocesses for the production of chemicals. Attractive features of biological systems include versatility, substrate selectivity, regioselectivity, chemoselectivity, enantioselectivity and catalysis at ambient temperatures and pressures. However, a challenge facing bioprocesses is cost competitiveness with chemical processes because capital assets associated with the existing commercial processes are high. The chemical industry will probably use biotechnology with existing feed stocks and processes to extract higher values from feed stocks, process by-products and waste streams. In this decade, bioprocesses that offer either a process or a product advantage over traditional chemical routes will become more widely used.

48 CFR 1301.671 - Assignment of program and project managers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... project managers. 1301.671 Section 1301.671 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE..., and Responsibilities 1301.671 Assignment of program and project managers. The Department's Program and Project Manager certification program for the assignment and certification of Program and Project Managers...

7 CFR 1210.311 - Programs and projects.

Code of Federal Regulations, 2010 CFR

2010-01-01

... PROMOTION PLAN Watermelon Research and Promotion Plan Definitions § 1210.311 Programs and projects. Programs and projects mean those research, development, advertising, or promotion programs or projects...

Multifunctional nanoparticle-protein conjugates with controllable bioactivity and pH responsiveness

NASA Astrophysics Data System (ADS)

Liu, Feng; Xue, Lulu; Yuan, Yuqi; Pan, Jingjing; Zhang, Chenjie; Wang, Hongwei; Brash, John L.; Yuan, Lin; Chen, Hong

2016-02-01

The modulation of protein activity is of significance for disease therapy, molecular diagnostics, and tissue engineering. Nanoparticles offer a new platform for the preparation of protein conjugates with improved protein properties. In the present work, Escherichia coli (E. coli) inorganic pyrophosphatase (PPase) and poly(methacrylic acid) (PMAA) were attached together to gold nanoparticles (AuNPs), forming AuNP-PPase-PMAA conjugates having controllable multi-biofunctionalities and responsiveness to pH. By treating with poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and regulating the pH, the bioactivity of the conjugate becomes ``on/off''-switchable. In addition, by taking advantage of the ability of AuNPs to undergo reversible aggregation/dispersion, the conjugates can be recycled and reused multiple times; and due to the shielding effect of the PMAA, the conjugated enzyme has high resistance to protease digestion. This approach has considerable potential in areas such as controlled delivery and release of drugs, biosensing, and biocatalysis.The modulation of protein activity is of significance for disease therapy, molecular diagnostics, and tissue engineering. Nanoparticles offer a new platform for the preparation of protein conjugates with improved protein properties. In the present work, Escherichia coli (E. coli) inorganic pyrophosphatase (PPase) and poly(methacrylic acid) (PMAA) were attached together to gold nanoparticles (AuNPs), forming AuNP-PPase-PMAA conjugates having controllable multi-biofunctionalities and responsiveness to pH. By treating with poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and regulating the pH, the bioactivity of the conjugate becomes ``on/off''-switchable. In addition, by taking advantage of the ability of AuNPs to undergo reversible aggregation/dispersion, the conjugates can be recycled and reused multiple times; and due to the shielding effect of the PMAA, the conjugated enzyme has high resistance to protease digestion. This approach has considerable potential in areas such as controlled delivery and release of drugs, biosensing, and biocatalysis. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr07436c

Properties and Synthetic Applications of Enzymes in Organic Solvents.

PubMed

Carrea; Riva

2000-07-03

Biotransformations already represent an effective and sometimes preferable alternative to chemical synthesis for the production of fine chemicals and optically active compounds. To further widen the versatility of the biological approach, the so-called "nonaqueous enzymology", which now represents an important area of research and biotechnological development, has emerged in the last ten years or so. This new methodology is especially suitable for the modification of precursors of pharmaceutical compounds and fine chemicals, which, in most cases, are insoluble or poorly soluble in water. Even though the idea of carrying out an enzymatic process in organic solvent was initially considered with scepticism, biocatalysis in such media is now investigated and exploited in numerous academic and industrial laboratories. One of the reasons that makes enzymatic catalysis in nonaqueous media so appealing, is the important new properties that enzymes exhibit in organic solvents. For example, they are often more stable and can catalyze reactions that are impossible or difficult in water. Furthermore, enzyme selectivity can also differ from that in water and can change, or even reverse, from one solvent to another. This phenomenon, which can be called "medium engineering", can be exploited as a valid alternative to protein engineering. The first part of this review examines the thermodynamic, kinetic, spectroscopic, and physical approaches that have been adopted to investigate the factors that affect activity, stability, structure, and selectivity of enzymes in organic solvents. These combined studies have brought the understanding of enzyme catalysis in organic solvents to a level almost comparable to that reached for biocatalysis in aqueous media. The second part surveys a number of the synthetic applications of enzymes in organic media, which span from the preparation of milligrams of specifically labeled compounds to the modification of fats on multiton scale and from the preparation of complex key intermediates for the pharmaceutical industry to the synthesis of polymers.

Amides in Nature and Biocatalysis.

PubMed

Pitzer, Julia; Steiner, Kerstin

2016-10-10

Amides are widespread in biologically active compounds with a broad range of applications in biotechnology, agriculture and medicine. Therefore, as alternative to chemical synthesis the biocatalytic amide synthesis is a very interesting field of research. As usual, Nature can serve as guide in the quest for novel biocatalysts. Several mechanisms for carboxylate activation involving mainly acyl-adenylate, acyl-phosphate or acyl-enzyme intermediates have been discovered, but also completely different pathways to amides are found. In addition to ribosomes, selected enzymes of almost all main enzyme classes are able to synthesize amides. In this review we give an overview about amide synthesis in Nature, as well as biotechnological applications of these enzymes. Moreover, several examples of biocatalytic amide synthesis are given. Copyright © 2016 Elsevier B.V. All rights reserved.

Directed evolution: an approach to engineer enzymes.

PubMed

Kaur, Jasjeet; Sharma, Rohit

2006-01-01

Directed evolution is being used increasingly in industrial and academic laboratories to modify and improve commercially important enzymes. Laboratory evolution is thought to make its biggest contribution in explorations of non-natural functions, by allowing us to distinguish the properties nurtured by evolution. In this review we report the significant advances achieved with respect to the methods of biocatalyst improvement and some critical properties and applications of the modified enzymes. The application of directed evolution has been elaborately demonstrated for protein solubility, stability and catalytic efficiency. Modification of certain enzymes for their application in enantioselective catalysis has also been elucidated. By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis.

Archaeal Enzymes and Applications in Industrial Biocatalysts.

PubMed

Littlechild, Jennifer A

2015-01-01

Archaeal enzymes are playing an important role in industrial biotechnology. Many representatives of organisms living in "extreme" conditions, the so-called Extremophiles, belong to the archaeal kingdom of life. This paper will review studies carried by the Exeter group and others regarding archaeal enzymes that have important applications in commercial biocatalysis. Some of these biocatalysts are already being used in large scale industrial processes for the production of optically pure drug intermediates and amino acids and their analogues. Other enzymes have been characterised at laboratory scale regarding their substrate specificity and properties for potential industrial application. The increasing availability of DNA sequences from new archaeal species and metagenomes will provide a continuing resource to identify new enzymes of commercial interest using both bioinformatics and screening approaches.

Challenges and prospects of xylitol production with whole cell bio-catalysis: A review.

PubMed

Dasgupta, Diptarka; Bandhu, Sheetal; Adhikari, Dilip K; Ghosh, Debashish

2017-04-01

Xylitol, as an alternative low calorie sweetener is well accepted in formulations of various confectioneries and healthcare products. Worldwide it is industrially produced by catalytic hydrogenation of pure d-xylose solution under high temperature and pressure. Biotechnological xylitol production is a potentially attractive replacement for chemical process, as it occurs under much milder process conditions and can be based on sugar mixtures derived from low-cost industrial and agri-waste. However, microbial fermentation route of xylitol production is not so far practiced industrially. This review highlights the challenges and prospects of biotechnological xylitol production considering possible genetic modifications of fermenting microorganisms and various aspects of industrial bioprocessing and product downstreaming. Copyright © 2017 Elsevier GmbH. All rights reserved.

Bacterial Diterpene Synthases: New Opportunities for Mechanistic Enzymology and Engineered Biosynthesis

PubMed Central

Smanski, Michael J.; Peterson, Ryan M.; Huang, Sheng-Xiong; Shen, Ben

2012-01-01

Diterpenoid biosynthesis has been extensively studied in plants and fungi, yet cloning and engineering diterpenoid pathways in these organisms remain challenging. Bacteria are emerging as prolific producers of diterpenoid natural products, and bacterial diterpene synthases are poised to make significant contributions to our understanding of terpenoid biosynthesis. Here we will first survey diterpenoid natural products of bacterial origin and briefly review their biosynthesis with emphasis on diterpene synthases (DTSs) that channel geranylgeranyl diphosphate to various diterpenoid scaffolds. We will then highlight differences of DTSs of bacterial and higher organism origins and discuss the challenges in discovering novel bacterial DTSs. We will conclude by discussing new opportunities for DTS mechanistic enzymology and applications of bacterial DTS in biocatalysis and metabolic pathway engineering. PMID:22445175

Introducing biobased ionic liquids as the nonaqueous media for enzymatic synthesis of phosphatidylserine.

PubMed

Bi, Yan-Hong; Duan, Zhang-Qun; Li, Xiang-Qian; Wang, Zhao-Yu; Zhao, Xi-Rong

2015-02-11

Biobased ionic liquids with cholinium as the cation and amino acids as the anions, which could be prepared from renewable biomaterials by simple neutralization reactions, have recently been described as promising and green solvents. Herein, they were successfully used as the reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for phosphatidylserine synthesis for the first time. Our results indicated that the highest phosphatidylserine yield of 86.5% was achieved. Moreover, 75% original activity of the enzyme was maintained after being used for 10 batches. The present work could be considered an alternative enzymatic strategy for preparing phosphatidylserine. Additionally, the excellent results make the biobased ionic liquids more promising candidates for use as environmentally friendly solvents in biocatalysis fields.

Progress in biocatalysis with immobilized viable whole cells: systems development, reaction engineering and applications.

PubMed

Polakovič, Milan; Švitel, Juraj; Bučko, Marek; Filip, Jaroslav; Neděla, Vilém; Ansorge-Schumacher, Marion B; Gemeiner, Peter

2017-05-01

Viable microbial cells are important biocatalysts in the production of fine chemicals and biofuels, in environmental applications and also in emerging applications such as biosensors or medicine. Their increasing significance is driven mainly by the intensive development of high performance recombinant strains supplying multienzyme cascade reaction pathways, and by advances in preservation of the native state and stability of whole-cell biocatalysts throughout their application. In many cases, the stability and performance of whole-cell biocatalysts can be highly improved by controlled immobilization techniques. This review summarizes the current progress in the development of immobilized whole-cell biocatalysts, the immobilization methods as well as in the bioreaction engineering aspects and economical aspects of their biocatalytic applications.

Issues in NASA Program and Project Management. Special Edition: A Collection of Papers on NASA Procedures and Guidance 7120.5A. Volume 14

NASA Technical Reports Server (NTRS)

Hoffman, Edward J. (Editor); Lawbaugh, William M. (Editor)

1998-01-01

A key aspect of NASA's new Strategic Management System is improving the way we plan, approve, execute and evaluate our programs and projects. To this end, NASA has developed the NASA Program and Project Management processes and Requirements-NASA Procedures and Guidelines (NPG) 7120.5A, which formally documents the "Provide Aerospace Products and Capabilities" crosscutting process, and defines the processes and requirements that are responsive to the Program/Project Management-NPD 7120.4A. The Program/Project Management-NPD 7120.4A, issued November 14, 1996, provides the policy for managing programs and projects in a new way that is aligned with the new NASA environment. An Agencywide team has spent thousands of hours developing the NASA Program and Project Management Processes and Requirements-NPG 7120.5A. We have created significant flexibility, authority and discretion for the program and project managers to exercise and carry out their duties, and have delegated the responsibility and the accountability for their programs and projects.

34 CFR 377.1 - What is the Demonstration Projects to Increase Client Choice Program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What is the Demonstration Projects to Increase Client... PROJECTS TO INCREASE CLIENT CHOICE PROGRAM General § 377.1 What is the Demonstration Projects to Increase Client Choice Program? The Demonstration Projects to Increase Client Choice Program is designed to...

7 CFR 1207.311 - Programs and projects.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan Definitions § 1207.311 Programs and projects. Programs and projects mean those research, development, advertising or promotion programs or projects developed by the...

34 CFR 664.5 - What definitions apply to the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2012 CFR

2012-07-01

... Projects Abroad Program? 664.5 Section 664.5 Education Regulations of the Offices of the Department of... PROJECTS ABROAD PROGRAM General § 664.5 What definitions apply to the Fulbright-Hays Group Projects Abroad... apply to this program: The following definitions apply to the Fulbright-Hays Group Projects Abroad...

34 CFR 664.5 - What definitions apply to the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2014 CFR

2014-07-01

... Projects Abroad Program? 664.5 Section 664.5 Education Regulations of the Offices of the Department of... PROJECTS ABROAD PROGRAM General § 664.5 What definitions apply to the Fulbright-Hays Group Projects Abroad... apply to this program: The following definitions apply to the Fulbright-Hays Group Projects Abroad...

34 CFR 664.5 - What definitions apply to the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2013 CFR

2013-07-01

... Projects Abroad Program? 664.5 Section 664.5 Education Regulations of the Offices of the Department of... PROJECTS ABROAD PROGRAM General § 664.5 What definitions apply to the Fulbright-Hays Group Projects Abroad... apply to this program: The following definitions apply to the Fulbright-Hays Group Projects Abroad...

34 CFR 664.5 - What definitions apply to the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... Projects Abroad Program? 664.5 Section 664.5 Education Regulations of the Offices of the Department of... PROJECTS ABROAD PROGRAM General § 664.5 What definitions apply to the Fulbright-Hays Group Projects Abroad... apply to this program: The following definitions apply to the Fulbright-Hays Group Projects Abroad...

34 CFR 664.5 - What definitions apply to the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Projects Abroad Program? 664.5 Section 664.5 Education Regulations of the Offices of the Department of... PROJECTS ABROAD PROGRAM General § 664.5 What definitions apply to the Fulbright-Hays Group Projects Abroad... apply to this program: The following definitions apply to the Fulbright-Hays Group Projects Abroad...

40 CFR 403.20 - Pretreatment Program Reinvention Pilot Projects Under Project XL.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Pretreatment Program Reinvention Pilot Projects Under Project XL. 403.20 Section 403.20 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... OF POLLUTION § 403.20 Pretreatment Program Reinvention Pilot Projects Under Project XL. The Approval...

10 CFR 420.32 - Program guidance/solicitation.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 3 2012-01-01 2012-01-01 false Program guidance/solicitation. 420.32 Section 420.32 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION STATE ENERGY PROGRAM Implementation of Special Projects... more project(s) under the special projects financial assistance with a detailed program guidance...

10 CFR 420.32 - Program guidance/solicitation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 3 2010-01-01 2010-01-01 false Program guidance/solicitation. 420.32 Section 420.32 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION STATE ENERGY PROGRAM Implementation of Special Projects... more project(s) under the special projects financial assistance with a detailed program guidance...

10 CFR 420.32 - Program guidance/solicitation.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 3 2014-01-01 2014-01-01 false Program guidance/solicitation. 420.32 Section 420.32 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION STATE ENERGY PROGRAM Implementation of Special Projects... more project(s) under the special projects financial assistance with a detailed program guidance...

10 CFR 420.32 - Program guidance/solicitation.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 3 2011-01-01 2011-01-01 false Program guidance/solicitation. 420.32 Section 420.32 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION STATE ENERGY PROGRAM Implementation of Special Projects... more project(s) under the special projects financial assistance with a detailed program guidance...

10 CFR 420.32 - Program guidance/solicitation.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 3 2013-01-01 2013-01-01 false Program guidance/solicitation. 420.32 Section 420.32 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION STATE ENERGY PROGRAM Implementation of Special Projects... more project(s) under the special projects financial assistance with a detailed program guidance...

42 CFR 50.208 - Program or project requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... GENERAL APPLICABILITY Sterilization of Persons in Federally Assisted Family Planning Projects § 50.208 Program or project requirements. (a) A program or project must, with respect to any sterilization...

The Many Dimensions of Program Management

NASA Technical Reports Server (NTRS)

Dean, Edwin B.

1992-01-01

For the purposes of this paper, program refers to a collection of activities or projects which must be performed according to a plan or schedule. The Space Exploration Initiative within the National Aeronautics and Space Administration (NASA) is an example. Dimensionality refers to both the various perspectives of a program and to the components within that perspective. It is, thus, appropriate to think of dimensions of dimensionality. For example, one dimension or perspective of a program is the projects which perform the program. Within the project dimension, the individual projects are the components of that dimensionality. The number of projects defines the spatial dimensionality of the project dimension. Thus, each perspective or dimension has a dimensionality of its own. The structure and associated values of all the various perspectives of a program define the program. A project refers to the collection of activities required to conceive, sell, design, develop, evaluate, produce, operate, support, evolve, and retire a given system. A project thus effects the life cycle of given system. A project is, thus, the system to conceive, sell, design, develop, evaluate, produce, operate, support, evolve, and retire a system. A program, thus, effects the life cycle of the collection of projects required to effect the collection of systems required to implement the program.

10 CFR 216.3 - Requests for assistance.

Code of Federal Regulations, 2014 CFR

2014-01-01

... associated with a program or project which may qualify as an eligible energy program or project and wishing... requesting DOE to determine whether a program or project maximizes domestic energy supplies and to find... description of the energy program or project for which assistance is requested and an assessment of its impact...

10 CFR 216.3 - Requests for assistance.

Code of Federal Regulations, 2013 CFR

2013-01-01

... associated with a program or project which may qualify as an eligible energy program or project and wishing... requesting DOE to determine whether a program or project maximizes domestic energy supplies and to find... description of the energy program or project for which assistance is requested and an assessment of its impact...

Clean Coal Technology Demonstration Program: Program Update 1998

DOE Office of Scientific and Technical Information (OSTI.GOV)

Assistant Secretary for Fossil Energy

1999-03-01

Annual report on the Clean Coal Technology Demonstration Program (CCT Program). The report address the role of the CCT Program, implementation, funding and costs, accomplishments, project descriptions, legislative history, program history, environmental aspects, and project contacts. The project descriptions describe the technology and provides a brief summary of the demonstration results.

48 CFR 301.607-70 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... General. In accordance with the Federal Acquisition Certification—Program and Project Managers (FAC-P/PM) program, HHS has established a certification program for Program or Project Managers. See HHS' Federal Acquisition Certification—Program and Project Managers Handbook (P/PM Handbook) for information on the methods...

Educational Project Management Instructional System. Module One. Project Management Executive Orientation.

ERIC Educational Resources Information Center

Cummings, C. Peter; Cook, Desmond L.

This module is the first in a self-instructional program designed to train public school personnel in how to manage educational projects. These may include federally funded projects (such as Title I projects), court-ordered programs (such as a desegregation plan), or locally initiated projects (such as a new math program). The purpose of this…

34 CFR 664.3 - Who is eligible to participate in projects funded under the Fulbright-Hays Group Projects Abroad...

Code of Federal Regulations, 2014 CFR

2014-07-01

... the Fulbright-Hays Group Projects Abroad Program? 664.3 Section 664.3 Education Regulations of the... EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM General § 664.3 Who is eligible to participate in projects funded under the Fulbright-Hays Group Projects Abroad Program? An individual is eligible to...

34 CFR 664.3 - Who is eligible to participate in projects funded under the Fulbright-Hays Group Projects Abroad...

Code of Federal Regulations, 2012 CFR

2012-07-01

... the Fulbright-Hays Group Projects Abroad Program? 664.3 Section 664.3 Education Regulations of the... EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM General § 664.3 Who is eligible to participate in projects funded under the Fulbright-Hays Group Projects Abroad Program? An individual is eligible to...

34 CFR 664.3 - Who is eligible to participate in projects funded under the Fulbright-Hays Group Projects Abroad...

Code of Federal Regulations, 2013 CFR

2013-07-01

... the Fulbright-Hays Group Projects Abroad Program? 664.3 Section 664.3 Education Regulations of the... EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM General § 664.3 Who is eligible to participate in projects funded under the Fulbright-Hays Group Projects Abroad Program? An individual is eligible to...

25 CFR 700.469 - Unallowable program and project costs.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 25 Indians 2 2013-04-01 2013-04-01 false Unallowable program and project costs. 700.469 Section... PROCEDURES Discretionary Funds § 700.469 Unallowable program and project costs. Costs for program or project... application for a demonstration effort under § 700.459 may include costs relating to the operation of the...

25 CFR 700.469 - Unallowable program and project costs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 2 2011-04-01 2011-04-01 false Unallowable program and project costs. 700.469 Section... PROCEDURES Discretionary Funds § 700.469 Unallowable program and project costs. Costs for program or project... application for a demonstration effort under § 700.459 may include costs relating to the operation of the...

25 CFR 700.469 - Unallowable program and project costs.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 25 Indians 2 2012-04-01 2012-04-01 false Unallowable program and project costs. 700.469 Section... PROCEDURES Discretionary Funds § 700.469 Unallowable program and project costs. Costs for program or project... application for a demonstration effort under § 700.459 may include costs relating to the operation of the...

25 CFR 700.469 - Unallowable program and project costs.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 2 2014-04-01 2014-04-01 false Unallowable program and project costs. 700.469 Section... PROCEDURES Discretionary Funds § 700.469 Unallowable program and project costs. Costs for program or project... application for a demonstration effort under § 700.459 may include costs relating to the operation of the...

48 CFR 301.607-78 - Contracting Officer designation of a Program/Project Manager as the Contracting Officer's...

Code of Federal Regulations, 2010 CFR

2010-10-01

... designation of a Program/Project Manager as the Contracting Officer's Technical Representative. 301.607-78... Contracting Officer designation of a Program/Project Manager as the Contracting Officer's Technical... acquisition. However, for those individuals serving as a Program or Project Manager under a FAC-P/PM...

Management of major system programs and projects. Handbook

NASA Technical Reports Server (NTRS)

1993-01-01

This Handbook establishes the detailed policies and processes for implementing NMI 7120.4, 'Management of Major System Programs and Projects'. It constitutes a comprehensive source of the specific policies and processes governing management of major development programs/projects and is intended as a resource to the entire program/project management (PPM) community.

Seven Key Principles of Program and Project Success: A Best Practices Survey

NASA Technical Reports Server (NTRS)

Bilardo, Vincent J.; Korte, John J.; Dankhoff, Walter; Langan, Kevin; Branscome, Darrell R.; Fragola, Joseph R.; Dugal, Dale J.; Gormley, Thomas J.; Hammond, Walter E.; Hollopeter, James J.;

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2012 CFR

2012-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2013 CFR

2013-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2014 CFR

2014-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2010 CFR

2010-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2011 CFR

2011-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

34 CFR 379.1 - What is the Projects With Industry (PWI) program?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 34 Education 2 2012-07-01 2012-07-01 false What is the Projects With Industry (PWI) program? 379.1... SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION PROJECTS WITH INDUSTRY General § 379.1 What is the Projects With Industry (PWI) program? The purpose of this program is to (a) Create...

34 CFR 379.1 - What is the Projects With Industry (PWI) program?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 34 Education 2 2013-07-01 2013-07-01 false What is the Projects With Industry (PWI) program? 379.1... SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION PROJECTS WITH INDUSTRY General § 379.1 What is the Projects With Industry (PWI) program? The purpose of this program is to (a) Create...

34 CFR 379.1 - What is the Projects With Industry (PWI) program?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 2 2014-07-01 2013-07-01 true What is the Projects With Industry (PWI) program? 379.1... SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION PROJECTS WITH INDUSTRY General § 379.1 What is the Projects With Industry (PWI) program? The purpose of this program is to (a) Create...

34 CFR 379.1 - What is the Projects With Industry (PWI) program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 2 2011-07-01 2010-07-01 true What is the Projects With Industry (PWI) program? 379.1... SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION PROJECTS WITH INDUSTRY General § 379.1 What is the Projects With Industry (PWI) program? The purpose of this program is to (a) Create...

A Novel Electrochemical Membrane Bioreactor as a Potential Net Energy Producer for Sustainable Wastewater Treatment

PubMed Central

Wang, Yun-Kun; Sheng, Guo-Ping; Shi, Bing-Jing; Li, Wen-Wei; Yu, Han-Qing

2013-01-01

One possible way to address both water and energy shortage issues, the two of major global challenges, is to recover energy and water resource from wastewater. Herein, a novel electrochemical membrane bioreactor (EMBR) was developed to recover energy from wastewater and meantime harvest clean water for reuse. With the help of the microorganisms in the biocatalysis and biodegradation process, net electricity could be recovered from a low-strength synthetic wastewater after estimating total energy consumption of this system. In addition, high-quality clean water was obtained for reuse. The results clearly demonstrate that, under the optimized operating conditions, it is possible to recover net energy from wastewater, while at the same time to harvest high-quality effluent for reuse with this novel wastewater treatment system. PMID:23689529

Functional Cellular Mimics for the Spatiotemporal Control of Multiple Enzymatic Cascade Reactions.

PubMed

Liu, Xiaoling; Formanek, Petr; Voit, Brigitte; Appelhans, Dietmar

2017-12-18

Next-generation therapeutic approaches are expected to rely on the engineering of biomimetic cellular systems that can mimic specific cellular functions. Herein, we demonstrate a highly effective route for constructing structural and functional eukaryotic cell mimics by loading pH-sensitive polymersomes as membrane-associated and free-floating organelle mimics inside the multifunctional cell membrane. Metabolism mimicry has been validated by performing successive enzymatic cascade reactions spatially separated at specific sites of cell mimics in the presence and absence of extracellular organelle mimics. These enzymatic reactions take place in a highly controllable, reproducible, efficient, and successive manner. Our biomimetic approach to material design for establishing functional principles brings considerable enrichment to the fields of biomedicine, biocatalysis, biotechnology, and systems biology. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pruski, Marek; Sadow, Aaron D.; Slowing, Igor I.

Catalysis research at the U.S. Department of Energy's (DOE's) National Laboratories covers a wide range of research topics in heterogeneous catalysis, homogeneous/molecular catalysis, biocatalysis, electrocatalysis, and surface science. Since much of the work at National Laboratories is funded by DOE, the research is largely focused on addressing DOE's mission to ensure America's security and prosperity by addressing its energy, environmental, and nuclear challenges through transformative science and technology solutions. The catalysis research carried out at the DOE National Laboratories ranges from very fundamental catalysis science, funded by DOE's Office of Basic Energy Sciences (BES), to applied research and development (R&D)more » in areas such as biomass conversion to fuels and chemicals, fuel cells, and vehicle emission control with primary funding from DOE's Office of Energy Efficiency and Renewable Energy.« less

Extremozymes: A Potential Source for Industrial Applications.

PubMed

Dumorné, Kelly; Córdova, David Camacho; Astorga-Eló, Marcia; Renganathan, Prabhaharan

2017-04-28

Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, and possess extraordinary properties of salt allowance, thermostability, and cold adaptivity. Extremozymes are very resistant to extreme conditions owing to their great solidity, and they pose new opportunities for biocatalysis and biotransformations, as well as for the development of the economy and new line of research, through their application. Thermophilic proteins, piezophilic proteins, acidophilic proteins, and halophilic proteins have been studied during the last few years. Amylases, proteases, lipases, pullulanases, cellulases, chitinases, xylanases, pectinases, isomerases, esterases, and dehydrogenases have great potential application for biotechnology, such as in agricultural, chemical, biomedical, and biotechnological processes. The study of extremozymes and their main applications have emerged during recent years.

Metagenomics as a Tool for Enzyme Discovery: Hydrolytic Enzymes from Marine-Related Metagenomes.

PubMed

Popovic, Ana; Tchigvintsev, Anatoly; Tran, Hai; Chernikova, Tatyana N; Golyshina, Olga V; Yakimov, Michail M; Golyshin, Peter N; Yakunin, Alexander F

2015-01-01

This chapter discusses metagenomics and its application for enzyme discovery, with a focus on hydrolytic enzymes from marine metagenomic libraries. With less than one percent of culturable microorganisms in the environment, metagenomics, or the collective study of community genetics, has opened up a rich pool of uncharacterized metabolic pathways, enzymes, and adaptations. This great untapped pool of genes provides the particularly exciting potential to mine for new biochemical activities or novel enzymes with activities tailored to peculiar sets of environmental conditions. Metagenomes also represent a huge reservoir of novel enzymes for applications in biocatalysis, biofuels, and bioremediation. Here we present the results of enzyme discovery for four enzyme activities, of particular industrial or environmental interest, including esterase/lipase, glycosyl hydrolase, protease and dehalogenase.

Formate production through biocatalysis

PubMed Central

Alissandratos, Apostolos; Kim, Hye-Kyung; Easton, Christopher J

2013-01-01

The generation of formate from CO2 provides a method for sequestration of this greenhouse gas as well as the production of a valuable commodity chemical and stabilized form of hydrogen fuel. Formate dehydrogenases are enzymes with the potential to catalyze this reaction; however they generally favor the reverse process, i.e., formate oxidation. By contrast, the formate dehydrogenase of the acetogen Clostridium carboxidivorans has been found to preferentially catalyze the reduction of CO2. This is in accord with its natural role to introduce CO2 as a carbon source in the Wood-Ljungdahl pathway. The direction of catalysis derives from the enzyme’s low affinity for formate. This enzyme is therefore an excellent candidate for biotechnological applications aimed at producing formic acid and derivative chemicals from CO2. PMID:23841981

Biotransformation of 20(R)-panaxatriol by Mucor racemosus and the anti-hepatic fibrosis activity of some products.

PubMed

Chen, Guangtong; Li, Jie; Yan, Sensen; Lin, Haijun; Wu, Juanjuan; Zhai, Xuguang; Song, Yan; Li, Jianlin

2017-08-01

Biocatalysis of 20(R)-panaxatriol (PT) was performed by the fungus Mucor racemosus. Six metabolites (1-6) including five new compounds were obtained, and their structures were elucidated as 20(R),25-epoxy-12β,24β-dihydroxydammaran-3,6-dione (2), 20(R),25-epoxy-12β,22β-dihydroxydammaran-3,6-dione (3), 20(R),25-epoxy-23β-hydroxydammaran-3,6,12-trione (4), 20(R),25-epoxy-12β,23α- dihydroxydammaran-3,6-dione (5), and 20(R),25-epoxy-12β-hydroxydammaran-3,6,23-trione (6) by spectroscopic analysis. Pharmacological studies revealed that compounds 2, 3 and 5 exhibited significant antihepatic fibrosis activity, while 4 and 6 showed cytotoxicity against HSC-T6 cells.

Archaeal Enzymes and Applications in Industrial Biocatalysts

PubMed Central

Littlechild, Jennifer A.

2015-01-01

Archaeal enzymes are playing an important role in industrial biotechnology. Many representatives of organisms living in “extreme” conditions, the so-called Extremophiles, belong to the archaeal kingdom of life. This paper will review studies carried by the Exeter group and others regarding archaeal enzymes that have important applications in commercial biocatalysis. Some of these biocatalysts are already being used in large scale industrial processes for the production of optically pure drug intermediates and amino acids and their analogues. Other enzymes have been characterised at laboratory scale regarding their substrate specificity and properties for potential industrial application. The increasing availability of DNA sequences from new archaeal species and metagenomes will provide a continuing resource to identify new enzymes of commercial interest using both bioinformatics and screening approaches. PMID:26494981

Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis.

PubMed

Kell, Douglas B; Swainston, Neil; Pir, Pınar; Oliver, Stephen G

2015-04-01

Because they mainly do not involve chemical changes, membrane transporters have been a Cinderella subject in the biotechnology of small molecule production, but this is a serious oversight. Influx transporters contribute significantly to the flux towards product, and efflux transporters ensure the accumulation of product in the much greater extracellular space of fermentors. Programmes for improving biotechnological processes might therefore give greater consideration to transporters than may have been commonplace. Strategies for identifying important transporters include expression profiling, genome-wide knockout studies, stress-based selection, and the use of inhibitors. In addition, modern methods of directed evolution and synthetic biology, especially those effecting changes in energy coupling, offer huge opportunities for increasing the flux towards extracellular product formation by transporter engineering. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

Maximizing the efficiency of multienzyme process by stoichiometry optimization.

PubMed

Dvorak, Pavel; Kurumbang, Nagendra P; Bendl, Jaroslav; Brezovsky, Jan; Prokop, Zbynek; Damborsky, Jiri

2014-09-05

Multienzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of the stoichiometry of the biocatalysts. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalyzing a five-step chemical conversion. Kinetic models of pathways with wild-type or engineered enzymes were built, and the enzyme stoichiometry of each pathway was optimized. Mathematical modeling and one-pot multienzyme experiments provided detailed insights into pathway dynamics, enabled the selection of a suitable engineered enzyme, and afforded high efficiency while minimizing biocatalyst loadings. Optimizing the stoichiometry in a pathway with an engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multienzyme processes. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Pericyclic reactions catalyzed by chorismate-utilizing enzymes

PubMed Central

Lamb, Audrey L.

2011-01-01

One of the fundamental questions of enzymology is how catalytic power is derived. This review focuses on recent developments in the structure-function relationships of chorismate-utilizing enzymes involved in siderophore biosynthesis to provide insight into the biocatalysis of pericyclic reactions. Specifically, salicylate synthesis by the two-enzyme pathway in Pseudomonas aeruginosa is examined. The isochorismate-pyruvate lyase is discussed in the context of its homologues, the chorismate mutases, and the isochorismate synthase is compared to its homologues in the MST-family (menaquinone, siderophore or tryptophan biosynthesis) of enzymes. The tentative conclusion is that the activities observed cannot be reconciled by inspection of the active site participants alone. Instead, individual activities must arise from unique dynamic properties of each enzyme that are tuned to promote specific chemistries. PMID:21823653

25 CFR 1000.240 - What construction programs included in an AFA are subject to this subpart?

Code of Federal Regulations, 2010 CFR

2010-04-01

..., coordination, responsibility for the construction project, day-to-day on-site management on site-management and administration of the project, which may include cost management, project budgeting, project scheduling and... Tribal, facilities and projects. (b) The following programs and activities are not construction programs...

Designing an Academic Project Management Program: A Collaboration between a University and a PMI Chapter

ERIC Educational Resources Information Center

Poston, Robin S.; Richardson, Sandra M.

2011-01-01

The demand for project management skills in industry is increasing resulting in a higher demand for project management educational programs. Universities are addressing industry demand by developing project management courses, degree offerings and certificate programs that focus on both technical and general project management skills. While…

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2010 CFR

2010-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2011 CFR

2011-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2013 CFR

2013-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2012 CFR

2012-07-01

... ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE CONTINUING AUTHORITIES PROGRAMS Pt. 263, App. A Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false History of Program and Project...

33 CFR Appendix A to Part 263 - History of Program and Project Limitations Continuing Authorities Program

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false History of Program and Project Limitations Continuing Authorities Program A Appendix A to Part 263 Navigation and Navigable Waters CORPS OF... Appendix A to Part 263—History of Program and Project Limitations Continuing Authorities Program Section...

Project management in the Apollo program: An interdisciplinary study

NASA Technical Reports Server (NTRS)

Drucker, E. E.; Pooler, W. S.; Wilemon, D. L.; Wood, B. D.

1972-01-01

Findings concerning project management in the NASA Apollo program are presented. The Apollo program in the context of the total NASA organization is examined along with the nature of project management and the manner in which project managers functioned in the Apollo program. The utilization of the in-house technical competence in the support of the Apollo program, and the formal and informal relationships between Apollo managers and the contractors are discussed.

22 CFR 216.2 - Applicability of procedures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... river basin development; (ii) Irrigation or water management projects, including dams and impoundments... projects, programs or activities authorized or approved by A.I.D. and to substantive amendments or extensions of ongoing projects, programs, or activities. (b) Exemptions. (1) Projects, programs or activities...

76 FR 12717 - Disability and Rehabilitation Research Projects and Centers Program; Field Initiated (FI...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-08

... DEPARTMENT OF EDUCATION Disability and Rehabilitation Research Projects and Centers Program; Field...; National Institute on Disability and Rehabilitation Research (NIDRR)--Disability and Rehabilitation Research Projects and Centers Program--Field Initiated (FI) Projects AGENCY: Office of Special Education...

Additionality and permanence standards in California's Forest Offset Protocol: A review of project and program level implications.

PubMed

Ruseva, T; Marland, E; Szymanski, C; Hoyle, J; Marland, G; Kowalczyk, T

2017-08-01

A key component of California's cap-and-trade program is the use of carbon offsets as compliance instruments for reducing statewide GHG emissions. Under this program, offsets are tradable credits representing real, verifiable, quantifiable, enforceable, permanent, and additional reductions or removals of GHG emissions. This paper focuses on the permanence and additionality standards for offset credits as defined and operationalized in California's Compliance Offset Protocol for U.S. Forest Projects. Drawing on a review of the protocol, interviews, current offset projects, and existing literature, we discuss how additionality and permanence standards relate to project participation and overall program effectiveness. Specifically, we provide an overview of offset credits as compliance instruments in California's cap-and-trade program, the timeline for a forest offset project, and the factors shaping participation in offset projects. We then discuss the implications of permanence and additionality at both the project and program levels. Largely consistent with previous work, we find that stringent standards for permanent and additional project activities can present barriers to participation, but also, that there may be a trade-off between project quality and quantity (i.e. levels of participation) when considering overall program effectiveness. We summarize what this implies for California's forest offset program and provide suggestions for improvements in light of potential program diffusion and policy learning. Copyright © 2017 Elsevier Ltd. All rights reserved.

23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false How will a bridge project be programmed for funding once... TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a bridge project be programmed for funding once eligibility has been determined? (a) All projects will be...

23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false How will a bridge project be programmed for funding once... TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a bridge project be programmed for funding once eligibility has been determined? (a) All projects will be...

Genetic Programming as Alternative for Predicting Development Effort of Individual Software Projects

PubMed Central

Chavoya, Arturo; Lopez-Martin, Cuauhtemoc; Andalon-Garcia, Irma R.; Meda-Campaña, M. E.

2012-01-01

Statistical and genetic programming techniques have been used to predict the software development effort of large software projects. In this paper, a genetic programming model was used for predicting the effort required in individually developed projects. Accuracy obtained from a genetic programming model was compared against one generated from the application of a statistical regression model. A sample of 219 projects developed by 71 practitioners was used for generating the two models, whereas another sample of 130 projects developed by 38 practitioners was used for validating them. The models used two kinds of lines of code as well as programming language experience as independent variables. Accuracy results from the model obtained with genetic programming suggest that it could be used to predict the software development effort of individual projects when these projects have been developed in a disciplined manner within a development-controlled environment. PMID:23226305

ePORT, NASA's Computer Database Program for System Safety Risk Management Oversight (Electronic Project Online Risk Tool)

NASA Technical Reports Server (NTRS)

Johnson, Paul W.

2008-01-01

ePORT (electronic Project Online Risk Tool) provides a systematic approach to using an electronic database program to manage a program/project risk management processes. This presentation will briefly cover the standard risk management procedures, then thoroughly cover NASA's Risk Management tool called ePORT. This electronic Project Online Risk Tool (ePORT) is a web-based risk management program that provides a common framework to capture and manage risks, independent of a programs/projects size and budget. It is used to thoroughly cover the risk management paradigm providing standardized evaluation criterion for common management reporting, ePORT improves Product Line, Center and Corporate Management insight, simplifies program/project manager reporting, and maintains an archive of data for historical reference.

Project Planning and Reporting

NASA Technical Reports Server (NTRS)

1982-01-01

Project Planning Analysis and Reporting System (PPARS) is automated aid in monitoring and scheduling of activities within project. PPARS system consists of PPARS Batch Program, five preprocessor programs, and two post-processor programs. PPARS Batch program is full CPM (Critical Path Method) scheduling program with resource capabilities. Can process networks with up to 10,000 activities.

Industrial Technology Modernization Program. Project 28. Automation of Receiving, Receiving Inspection and Stores

DTIC Science & Technology

1987-06-15

001 GENERAL DYNAMICS 00 FORT WORTH DIVISION INDUSTRIAL TECHNOLOGY MODERNIZATION PROGRAM Phase 2 Final Project Repc t JUNG 0 ?7 PROJECT 28 AUTOMATION...DYNAMICS FORT WORTH DIVISION INDUSTRIAL TECHNOLOGY MODERNIZATION PROGRAM Phase 2 Final Project Report PROJECT 28 AUTOMATION OF RECEIVING, RECEIVING...13 6 PROJECT ASSUMPTIONS 20 7 PRELIMINARY/FINAL DESIGN AND FINDINGS 21 8 SYSTEM/EQUIPMENT/MACHINING SPECIFICATIONS 37 9 VENDOR/ INDUSTRY ANALYSIS

Environmental Management Science Program Workshop

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1998-07-01

This program summary book is a compendium of project summaries submitted by principal investigators in the Environmental Management Science Program and Environmental Management/Energy Research Pilot Collaborative Research Program (Wolf-Broido Program). These summaries provide information about the most recent project activities and accomplishments. All projects will be represented at the workshop poster sessions, so you will have an opportunity to meet with the researchers. The projects will be presented in the same order at the poster session as they are presented in this summary book. Detailed questions about an individual project may be directed to the investigators involved.

Computer Aided Management for Information Processing Projects.

ERIC Educational Resources Information Center

Akman, Ibrahim; Kocamustafaogullari, Kemal

1995-01-01

Outlines the nature of information processing projects and discusses some project management programming packages. Describes an in-house interface program developed to utilize a selected project management package (TIMELINE) by using Oracle Data Base Management System tools and Pascal programming language for the management of information system…

Intergenerational Projects: Idea Book.

ERIC Educational Resources Information Center

Clay, Rebecca; Ventura-Merkel, Cathy; Eades-Goudy, Dianne; Dubich, Teresa

This book profiles 74 intergenerational programs in the United States. The programs range from basic tutoring projects to a sophisticated corporate-based day care center. Project selection was based on replicatable programs involving mutually beneficial exchanges. Grouped by subjects, profiles include programs targeting both young and old. Most…

Atmospheric Science Program. Summaries of research in FY 1994

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-06-01

This report provides descriptions for all projects funded by ESD under annual contracts in FY 1994. Each description contains the project`s title; three-year funding history (in thousands of dollars); the contract period over which the funding applies; the name(s) of the principal investigator(s); the institution(s) conducting the projects; and the project`s objectives, products, approach, and results to date (for most projects older than one year). Project descriptions are categorized within the report according to program areas: atmospheric chemistry, atmospheric dynamics, and support operations. Within these categories, the descriptions are ordered alphabetically by principal investigator. Each program area is preceded bymore » a brief text that defines the program area, states it goals and objectives, lists principal research questions, and identifies program managers. Appendixes provide the addresses and telephone numbers of the principal investigators and define the acronyms used.« less

34 CFR 645.1 - What is the Upward Bound Program?

Code of Federal Regulations, 2013 CFR

2013-07-01

... the following three types of projects: (1) Regular Upward Bound projects. (2) Upward Bound Math and Science Centers. (3) Veterans Upward Bound projects. (Authority: 20 U.S.C. 1070a-11 and 1070a-13) ...) The Upward Bound Program provides Federal grants to projects designed to generate in program...

34 CFR 645.1 - What is the Upward Bound Program?

Code of Federal Regulations, 2014 CFR

2014-07-01

... the following three types of projects: (1) Regular Upward Bound projects. (2) Upward Bound Math and Science Centers. (3) Veterans Upward Bound projects. (Authority: 20 U.S.C. 1070a-11 and 1070a-13) ...) The Upward Bound Program provides Federal grants to projects designed to generate in program...

34 CFR 645.1 - What is the Upward Bound Program?

Code of Federal Regulations, 2012 CFR

2012-07-01

... the following three types of projects: (1) Regular Upward Bound projects. (2) Upward Bound Math and Science Centers. (3) Veterans Upward Bound projects. (Authority: 20 U.S.C. 1070a-11 and 1070a-13) ...) The Upward Bound Program provides Federal grants to projects designed to generate in program...

34 CFR 645.1 - What is the Upward Bound Program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... the following three types of projects: (1) Regular Upward Bound projects. (2) Upward Bound Math and Science Centers. (3) Veterans Upward Bound projects. (Authority: 20 U.S.C. 1070a-11 and 1070a-13) ...) The Upward Bound Program provides Federal grants to projects designed to generate in program...

7 CFR 1486.103 - Are regional projects possible under the program?

Code of Federal Regulations, 2010 CFR

2010-01-01

... consideration provided such projects target qualifying emerging markets in the specified region. CCC may consider activities which target qualified emerging markets in a specific region, but are conducted in a... MARKETS PROGRAM General Information § 1486.103 Are regional projects possible under the program? Projects...

Project SAIL: A Summer Program Brings History Alive for Students.

ERIC Educational Resources Information Center

Hollingsworth, Patricia

2001-01-01

This project describes Project SAIL (Schools for Active Interdisciplinary Learning), a federally funded project providing in-depth staff development during a 3-week summer program for teachers, parents, and their gifted/talented economically disadvantaged students. The program theme, "Searching for Patterns in History," has been used with students…

QA program plan plutonium stabilization and handling project W-460

DOE Office of Scientific and Technical Information (OSTI.GOV)

SCHULTZ, J.W.

This Quality Assurance Program Plan (QAPP) identifies Project Quality Assurance (QA) program requirements for all parties participating in the design, procurement, demolition, construction, installation, inspection and testing for Project W-460.

23 CFR 668.113 - Program and project procedures.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Program and project procedures. 668.113 Section 668.113 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal-Aid Highways § 668.113 Program and project procedures. (a...

Design of training programs for a positive youth development program: Project P.A.T.H.S. in Hong Kong.

PubMed

Shek, Daniel T L; Chak, Yammy L Y

2010-01-01

To facilitate the implementation of the Secondary 1 to Secondary 3 program of the Project P.A.T.H.S. in Hong Kong, systematic training programs are designed for the potential program implementers. The rationales, objectives and design of the Secondary 1 to Secondary 3 training programs are outlined in this paper. The training programs cover theories of adolescent development, positive youth development, background and curricula of the Project P.A.T.H.S., factors affecting program implementation quality and evaluation of the project. Besides introducing the curriculum units, the training programs also focus on nature of learning and related theories (particularly experiential learning), teaching methods and instructional techniques, motivating students, and classroom management.

Aviation Safety Program: Weather Accident Prevention (WxAP) Project Overview and Status

NASA Technical Reports Server (NTRS)

Nadell, Shari-Beth

2003-01-01

This paper presents a project overview and status for the Weather Accident Prevention (WxAP) aviation safety program. The topics include: 1) Weather Accident Prevention Project Background/History; 2) Project Modifications; 3) Project Accomplishments; and 4) Project's Next Steps.

MG SPROUTS: A Project-in-a-Box Approach to Educational Programming

ERIC Educational Resources Information Center

Dorn,Sheri; Slagle, Krissy

2016-01-01

MG SPROUTS was developed as a "project-in-a-box" program, a self-contained educational programming tool for Extension agents working with master gardener Extension volunteers (MGEVs). The program design incorporates programmatic materials and project management materials and follows best management practices for volunteer management. MG…

Employment Projections and Program Priorities. AIR 1999 Annual Forum Paper.

ERIC Educational Resources Information Center

Rosenthal, Dan; Collier, Kitty

This study compared occupational projections for Alabama with graduation rates in corresponding academic programs to provide a context for state and institutional policy decisions on new program initiatives and to comply with recent program viability legislation. The study examined number of degrees conferred, employment projections, and…

Phase C/D program development plan. Volume 1: Program plan

NASA Technical Reports Server (NTRS)

1971-01-01

The Phase C/D definition of the Modular Space Station has been developed. The modular approach selected during the option period was evaluated, requirements were defined, and program definition and preliminary design were accomplished. The Space Station Project is covered in depth, the research applications module is limited to a project-level definition, and the shuttle operations are included for interface requirements identification, scheduling, and costing. Discussed in detail are: (1) baseline program and project descriptions; (2) phase project planning; (3) modular space station program schedule; (4) program management plan; (5) operations; (6) facilities; (7) logistics; and (8) manpower.

5 CFR 470.301 - Program expectations.

Code of Federal Regulations, 2010 CFR

2010-01-01

....301 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PERSONNEL MANAGEMENT RESEARCH PROGRAMS AND DEMONSTRATIONS PROJECTS Regulatory Requirements Pertaining to Demonstration Projects § 470.301 Program expectations. (a) Demonstration projects permit the Office of Personnel...

Water-resources investigations in Pennsylvania; programs and activities of the U.S. Geological Survey, 1993

USGS Publications Warehouse

McLanahan, L. O.

1993-01-01

Current activities of the Pennsylvania District of the USGS are described and include information on current projects, such as project objectives, approach, progress and plans, project location, cooperators, period of project, and project chief. Basic-data programs for surface water, ground water, and quality of water also are described. Also included is information on the basic mission and programs of the USGS; program funding and cooperation for fiscal year 1993; the USGS water- data program, National Water-Data Exchange, and National Water-Data Storage and Retrieval System; and Pennsylvania data-collection programs and hydrologic investigations. List of publications of the Pennsylvania District and maps published by the USGS, as well as information on how to obtain them, are included.

Lightning Mapper Sensor Lens Assembly S.O. 5459: Project Management Plan

NASA Technical Reports Server (NTRS)

Zeidler, Janet

1999-01-01

Kaiser Electro-Optics, Inc. (KEO) has developed this Project Management Plan for the Lightning Mapper Sensor (LMS) program. KEO has integrated a team of experts in a structured program management organization to meet the needs of the LMS program. The project plan discusses KEO's approach to critical program elements including Program Management, Quality Assurance, Configuration Management, and Schedule.

Civilian Agency Industry Working Group EVM World Update

NASA Technical Reports Server (NTRS)

Kerby, Jerald

2013-01-01

Objectives include: Promote the use of standards ]based, objective, and quantitative systems for managing projects and programs in the federal government. Understand how civilian agencies in general, manage their projects and programs. Project management survey expected to go out soon to civilian agencies. Describe how EVM and other best practices can be applied by the government to better manage its project and programs irrespective of whether work is contracted out or the types of contracts employed. Develop model policies aimed at project and program managers that are transportable across the government.

Two horses of a different color: CSM in Thailand and Colombia.

PubMed

1985-01-01

The Colombian Association for Family Welfare (PROFAMILIA) and Indonesia's Community Development Association (PDA) operate social marketing projects which might more appropriately be called social sales projects. Unlike other social marketing projects, these 2 projects seek profits from their contraceptive sales. The profits are then used to subsidize other programs operated by these 2 nonprofit organizations. Indonesia's PDA initiated its sales project in 1974. It operates both an urban contraceptive retail sales (CRS) program and a rural community-based distribution sales (CBD) program. The CRS program sells 3 types of condoms, which it delivers directly to the 1750 retailers involved in the program. The condoms sell for US$.03-US$.07, somewhat more than condoms sold in most social marketing projects. The CBD project covers 10,200 villages in 157 of Indonesia's 620 districts. Each village has a CBD volunteer who sells oral contraceptives (OCs) and condoms and also promotes family planning and rural development. The program sells Norinyl, Ovostat, and Eugynon for US$.19-US$.30/cycle. PDA runs other profit-making projects. For example, it sells promotional T-shirts and calendars at its vasectomy clinics and through its CBD program. PDA also established the taxable Population and Development Corporation, which engages in marketing activities. Profits from the corporation are channeled back to PDA. PROFAMILIA also operates both an urban sales program and a rural community-based distribution program. In the urban program, condoms are sold at normal retail prices and OCs at prices 30% below retail prices. The profits derived form the urban sales are used to subsidize the rural project. In 1981, the urban program began selling 6 noncontraceptive products, e.g., distilled water and disposable diapers. Currently, the program sells only distilled water, as the other products failed to yield a profit. A table provides sales information for both the PDA and PROFAMILIA programs.

40 CFR 122.25 - Aquaculture projects (applicable to State NPDES programs, see § 123.25).

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Aquaculture projects (applicable to... projects (applicable to State NPDES programs, see § 123.25). (a) Permit requirement. Discharges into aquaculture projects, as defined in this section, are subject to the NPDES permit program through section 318...

34 CFR 387.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What types of projects are authorized under this... INNOVATIVE TRAINING What Kinds of Projects Does the Department of Education Assist Under This Program? § 387.10 What types of projects are authorized under this program? The Experimental and Innovative Training...

23 CFR 140.603 - Programs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of bonds shall be prepared and submitted to FHWA. Project designations shall be the same as for regular Federal-aid projects except that the prefix letter “B” for bond issue shall be used as the first letter of...

Re-evaluation of Montana's air quality program.

DOT National Transportation Integrated Search

2013-08-01

This project examined the Montana DOTs current methods for determining projects for the Montana Air and Congestion Initiative (MACI) program, and made recommendations to improve and implement this program. A major project objective was to keep the...

NASA Space Flight Program and Project Management Handbook

NASA Technical Reports Server (NTRS)

Blythe, Michael P.; Saunders, Mark P.; Pye, David B.; Voss, Linda D.; Moreland, Robert J.; Symons, Kathleen E.; Bromley, Linda K.

2014-01-01

This handbook is a companion to NPR 7120.5E, NASA Space Flight Program and Project Management Requirements and supports the implementation of the requirements by which NASA formulates and implements space flight programs and projects. Its focus is on what the program or project manager needs to know to accomplish the mission, but it also contains guidance that enhances the understanding of the high-level procedural requirements. (See Appendix C for NPR 7120.5E requirements with rationale.) As such, it starts with the same basic concepts but provides context, rationale, guidance, and a greater depth of detail for the fundamental principles of program and project management. This handbook also explores some of the nuances and implications of applying the procedural requirements, for example, how the Agency Baseline Commitment agreement evolves over time as a program or project moves through its life cycle.

Emergency Immigration Education Act Programs. Summer ESL Welcome Program for Students of Limited English Proficiency, Summer Bilingual Program, Projects Omega, Wise, and Bell. Summer 1994. OER Report.

ERIC Educational Resources Information Center

Roman, Elliott M.

The Emergency Immigration Education Act supported three distinct programs in New York City in the summer of 1994: (1) the Summer English as a Second Language (ESL) Welcome Program for Students of Limited English Proficiency; (2) the Summer Bilingual Program; and (3) Projects Omega, Wise, and Bell. The projects served 3,443 students in all. The…

24 CFR 266.200 - Eligible projects.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Eligible projects. 266.200 Section... FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Program Requirements § 266.200 Eligible projects. (a) Minimum project size. Projects insured under this part must consist of...

24 CFR 266.200 - Eligible projects.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Eligible projects. 266.200 Section... FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Program Requirements § 266.200 Eligible projects. (a) Minimum project size. Projects insured under this part must consist of...

24 CFR 266.200 - Eligible projects.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Eligible projects. 266.200 Section... FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Program Requirements § 266.200 Eligible projects. (a) Minimum project size. Projects insured under this part must consist of...

24 CFR 266.200 - Eligible projects.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Eligible projects. 266.200 Section... FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Program Requirements § 266.200 Eligible projects. (a) Minimum project size. Projects insured under this part must consist of...

The DEVELOP National Program's Strategy for Communicating Applied Science Outcomes

NASA Astrophysics Data System (ADS)

Childs-Gleason, L. M.; Ross, K. W.; Crepps, G.; Favors, J.; Kelley, C.; Miller, T. N.; Allsbrook, K. N.; Rogers, L.; Ruiz, M. L.

2016-12-01

NASA's DEVELOP National Program conducts rapid feasibility projects that enable the future workforce and current decision makers to collaborate and build capacity to use Earth science data to enhance environmental management and policy. The program communicates its results and applications to a broad spectrum of audiences through a variety of methods: "virtual poster sessions" that engage the general public through short project videos and interactive dialogue periods, a "Campus Ambassador Corps" that communicates about the program and its projects to academia, scientific and policy conference presentations, community engagement activities and end-of-project presentations, project "hand-offs" providing results and tools to project partners, traditional publications (both gray literature and peer-reviewed), an interactive website project gallery, targeted brochures, and through multiple social media venues and campaigns. This presentation will describe the various methods employed by DEVELOP to communicate the program's scientific outputs, target audiences, general statistics, community response and best practices.

75 FR 1408 - Pick-Sloan Missouri Basin Program, Eastern and Western Division Proposed Project Use Power Rate

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-11

... of Proposed Pick-Sloan Missouri Basin Program, Eastern and Western Divisions, Project Use Power Rate...) for Project Use Power for the Pick-Sloan Missouri Basin Program (P-SMBP), Eastern and Western... DEPARTMENT OF THE INTERIOR Bureau of Reclamation Pick-Sloan Missouri Basin Program, Eastern and...

Selection and Validation of Model Early Childhood Projects: Final Report.

ERIC Educational Resources Information Center

Stock, John R.; And Others

Presented is the final report of a research program to select and describe outstanding Handicapped Children's Early Education Program (HCEEP) projects. Projects were analyzed in terms of 14 components of an HCEEP program (such as screening, programing, and inservice training). A detailed discussion of model selection and validation is appendixed.)…

76 FR 29253 - Part F Special Projects of National Significance Program Cooperative Agreement Under the Ryan...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-20

... Special Projects of National Significance Program Cooperative Agreement Under the Ryan White HIV/AIDS...-Competitive Supplemental Award of the Ryan White HIV/AIDS Program Part F Funds for the Special Projects of National Significance (SPNS) Program's Enhancing Linkages to HIV Care and Treatment in Jail Settings...

34 CFR 389.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What types of projects are authorized under this program? 389.10 Section 389.10 Education Regulations of the Offices of the Department of Education... Program? § 389.10 What types of projects are authorized under this program? Rehabilitation Continuing...

34 CFR 389.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 2 2011-07-01 2010-07-01 true What types of projects are authorized under this program? 389.10 Section 389.10 Education Regulations of the Offices of the Department of Education (Continued... Program? § 389.10 What types of projects are authorized under this program? Rehabilitation Continuing...

Association between postgraduate year 2 residency program characteristics and primary project publication.

PubMed

Swanson, Joseph M; Shafeeq, Hira; Hammond, Drayton A; Li, Chenghui; Devlin, John W

2018-03-15

The association among residency program and research mentor characteristics, program director perceptions, and the publication of the primary research project for postgraduate year 2 (PGY2) graduates was assessed. Using a validated electronic survey, residency program directors (RPDs) of critical care PGY2 graduates were asked about primary research project publication success, program and research project mentor characteristics, and RPDs' perceptions surrounding project completion. All 55 RPDs surveyed responded; 44 (79%) reported being a research project mentor. PGY2 research project publications in 2011 and 2012 totaled 26 (37%) and 27 (35%), respectively. A significant relationship existed between research project publication and the number of residents in the program ( p < 0.01); the perception among the RPDs that research project publication is important to their employer ( p < 0.01); and the research mentor's funding source ( p = 0.04), employer ( p < 0.01), number of prior publications ( p = 0.01), and research training ( p < 0.01). Variables independently associated with the publication of 2 or more research projects versus no publications included the number of graduates in the PGY2 program (odds ratio [OR], 5.6; p < 0.01), the RPD's perception that the employer valued research project publication (OR, 10.2; p < 0.01), and the number of prior publications by the least-experienced research mentor (OR, 23.5; p = 0.01). The publication of 1 research project versus no research projects was also independently associated with the RPD's perception that the employer valued research project publication (OR, 5.1; p = 0.04). A survey of RPDs of critical care PGY2 residents found that the number of PGY2 residents, the number of publications by the least experienced research mentor, and the perception that publishing the residents' research projects is important to the employer were independently associated with publication rates of residency research projects. Copyright © 2018 by the American Society of Health-System Pharmacists, Inc. All rights reserved.

Regional Interstate Planning Project Program . . . Vol. IX. California Program Evaluation Improvement Project. Seminar Report.

ERIC Educational Resources Information Center

Dearmin, Evalyn, Ed.; And Others

Program evaluation strategies and techniques based on materials developed by the California Evaluation Improvement Project were discussed at this meeting of the Regional Interstate Planning Project (RIPP). RIPP members represent the State Departments of Education of ten western states, and have met periodically over the past nine years to discuss…

Psychrophiles

NASA Astrophysics Data System (ADS)

Siddiqui, Khawar S.; Williams, Timothy J.; Wilkins, David; Yau, Sheree; Allen, Michelle A.; Brown, Mark V.; Lauro, Federico M.; Cavicchioli, Ricardo

2013-05-01

Psychrophilic (cold-adapted) microorganisms make a major contribution to Earth's biomass and perform critical roles in global biogeochemical cycles. The vast extent and environmental diversity of Earth's cold biosphere has selected for equally diverse microbial assemblages that can include archaea, bacteria, eucarya, and viruses. Underpinning the important ecological roles of psychrophiles are exquisite mechanisms of physiological adaptation. Evolution has also selected for cold-active traits at the level of molecular adaptation, and enzymes from psychrophiles are characterized by specific structural, functional, and stability properties. These characteristics of enzymes from psychrophiles not only manifest in efficient low-temperature activity, but also result in a flexible protein structure that enables biocatalysis in nonaqueous solvents. In this review, we examine the ecology of Antarctic psychrophiles, physiological adaptation of psychrophiles, and properties of cold-adapted proteins, and we provide a view of how these characteristics inform studies of astrobiology.

What are the Limitations of Enzymes in Synthetic Organic Chemistry?

PubMed

Reetz, Manfred T

2016-12-01

Enzymes have been used in organic chemistry and biotechnology for 100 years, but their widespread application has been prevented by a number of limitations, including the often-observed limited thermostability, narrow substrate scope, and low or wrong stereo- and/or regioselectivity. Directed evolution provides a means to address and generally solve these problems, especially since recent methodology development has made this protein engineering method faster, more efficient, and more reliable than in the past. This Darwinian approach to asymmetric catalysis has led to a number of industrial applications. Metabolic-pathway engineering, mutasynthesis, and fermentation are likewise enzyme-based techniques that enrich chemistry. This account outlines the scope, and particularly, the limitations, of biocatalysis. The complementary nature of enzymes and man-made catalysts is emphasized. © 2016 The Chemical Society of Japan & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Uncoupling binding of substrate CO from turnover by vanadium nitrogenase.

PubMed

Lee, Chi Chung; Fay, Aaron W; Weng, Tsu-Chien; Krest, Courtney M; Hedman, Britt; Hodgson, Keith O; Hu, Yilin; Ribbe, Markus W

2015-11-10

Biocatalysis by nitrogenase, particularly the reduction of N2 and CO by this enzyme, has tremendous significance in environment- and energy-related areas. Elucidation of the detailed mechanism of nitrogenase has been hampered by the inability to trap substrates or intermediates in a well-defined state. Here, we report the capture of substrate CO on the resting-state vanadium-nitrogenase in a catalytically competent conformation. The close resemblance of this active CO-bound conformation to the recently described structure of CO-inhibited molybdenum-nitrogenase points to the mechanistic relevance of sulfur displacement to the activation of iron sites in the cofactor for CO binding. Moreover, the ability of vanadium-nitrogenase to bind substrate in the resting-state uncouples substrate binding from subsequent turnover, providing a platform for generation of defined intermediate(s) of both CO and N2 reduction.

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions.

PubMed

Nikel, Pablo I; Chavarría, Max; Danchin, Antoine; de Lorenzo, Víctor

2016-10-01

The soil bacterium Pseudomonas putida is endowed with a central carbon metabolic network capable of fulfilling high demands of reducing power. This situation arises from a unique metabolic architecture that encompasses the partial recycling of triose phosphates to hexose phosphates-the so-called EDEMP cycle. In this article, the value of P. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed. The biochemical properties that make this bacterium adequate for hosting biotransformations involving redox reactions as well as toxic compounds and intermediates are discussed. Finally, novel developments and open questions in the continuous quest for an optimal microbial cell factory are presented at the light of current and future needs in the area of biocatalysis. Copyright © 2016 Elsevier Ltd. All rights reserved.

Biodecontamination of concrete

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rogers, R.D.

1995-12-31

This paper describes the development and results of a demonstration for a continuous bioprocess for mixed waste treatment. A key element of the process is a unique microbial strain, which tolerates high levels of aromatic solvents and surfactants. This microorganism is the biocatalysis of the continuous flow system designed for processing stored liquid scintillation wastes. During the past year, a process demonstration has been conducted on commercial formulation of liquid scintillation cocktails (LSQ). Based on data obtained from this demonstration, the Ohio Environmental Protection Agency granted the Mound Applied Technologies Laboratory a treatability permit allowing the limited processing of actualmore » mixed waste. Since August 1994, the system has been successfully processing stored {open_quotes}hot{close_quotes} LSC waste. This paper discusses the bioprocess, rates of processing, effluent, and implications of bioprocessing for mixed waste management.« less

Biosynthesis of monomers for plastics from renewable oils.

PubMed

Lu, Wenhua; Ness, Jon E; Xie, Wenchun; Zhang, Xiaoyan; Minshull, Jeremy; Gross, Richard A

2010-11-03

Omega-hydroxyfatty acids are excellent monomers for synthesizing a unique family of polyethylene-like biobased plastics. However, ω-hydroxyfatty acids are difficult and expensive to prepare by traditional organic synthesis, precluding their use in commodity materials. Here we report the engineering of a strain of the diploid yeast Candida tropicalis to produce commercially viable yields of ω-hydroxyfatty acids. To develop the strain we identified and eliminated 16 genes encoding 6 cytochrome P450s, 4 fatty alcohol oxidases, and 6 alcohol dehydrogenases from the C. tropicalis genome. We also show that fatty acids with different chain lengths and degrees of unsaturation can be more efficiently oxidized by expressing different P450s within this strain background. Biocatalysis using engineered C. tropicalis is thus a potentially attractive biocatalytic platform for producing commodity chemicals from renewable resources.

Chemical vs. biotechnological synthesis of C13-apocarotenoids: current methods, applications and perspectives.

PubMed

Cataldo, Vicente F; López, Javiera; Cárcamo, Martín; Agosin, Eduardo

2016-07-01

Apocarotenoids are natural compounds derived from the oxidative cleavage of carotenoids. Particularly, C13-apocarotenoids are volatile compounds that contribute to the aromas of different flowers and fruits and are highly valued by the Flavor and Fragrance industry. So far, the chemical synthesis of these terpenoids has dominated the industry. Nonetheless, the increasing consumer demand for more natural and sustainable processes raises an interesting opportunity for bio-production alternatives. In this regard, enzymatic biocatalysis and metabolically engineered microorganisms emerge as attractive biotechnological options. The present review summarizes promising bioengineering approaches with regard to chemical production methods for the synthesis of two families of C13-apocarotenoids: ionones/dihydroionones and damascones/damascenone. We discuss each method and its applicability, with a thorough comparative analysis for ionones, focusing on the production process, regulatory aspects, and sustainability.

Evaluation of programs for adolescents with greater psychosocial needs: community-based Project P.A.T.H.S. in Hong Kong.

PubMed

Shek, Daniel T L; Law, Moon Y M

2017-02-01

This study examined the perceptions of the Tier 2 Program of the Project P.A.T.H.S. (Positive Adolescent Training through Holistic Social programmes) among Chinese secondary school students displaying greater psychosocial needs. Results showed that participants held positive views of program qualities, implementer qualities, as well as the perceived effectiveness of the program. Significant grade differences in terms of various indicators of satisfaction related to program qualities, implementer qualities, and program effectiveness were found. Correlation analyses showed that there were significant inter-relationships amongst program qualities, implementer qualities and program effectiveness. In line with the predictions, both program qualities and implementer qualities were significant predictors of perceived program effectiveness. The present study provides support for the perceived effectiveness of the Tier 2 Program of the Project P.A.T.H.S. in the community-based project context.

Perceived value and outcomes of residency projects.

PubMed

Murphy, J E; Downhour, N

2001-05-15

Residency program directors' attitudes toward residency projects were studied. A questionnaire about the residency project experience was mailed in January 2000 to 446 pharmacy practice residency program and specialty residency program directors in the program database of the American Society of Health-System Pharmacists. Recipients responded to opinion statements on a 5-point scale. Responses to the opinion statements were separated into seven categories for analysis. A total of 278 usable questionnaires were returned, for a raw response rate of 63.6%. During the preceding three years, residency directors had served as primary advisors on 917 projects; 171 had served as advisor on at least one of every type of project allowed in the accreditation standards. Of the 917 projects, 364 were presented at national professional meetings, 124 were published, and 484 were believed to have resulted in a positive change in pharmacy services. There were no significant differences in total response scores among any of the subgroups analyzed. There was strong agreement that residency projects were valuable and should continue to be part of the residency program experience. The directors' views of the importance of original research as a project option were more neutral. Overall residency program directors had positive perceptions of the value of residency projects to both residents and institutions and believed that they should continue to be a requirement of residency programs.

Kodak Skills Enhancement Program. U.S. Department of Education National Workplace Literacy Project. Final Report.

ERIC Educational Resources Information Center

Beaudin, Bart P.

The Kodak Skills Enhancement program was a workplace literacy project funded through the U.S. Department of Education's National Workplace Literacy Program. The project goals were as follows: (1) establish a positive climate within the Kodak corporate environment to ensure program effectiveness by garnering support at all levels; (2) determine the…

National Evaluation of the Even Start Family Literacy Program: Report on Migrant Even Start Projects.

ERIC Educational Resources Information Center

Levin, Marjorie; Gamse, Beth; Swartz, Janet; Tao, Fumiyo; Tarr, Hope

In fall 1994, 14 state Migrant Education Programs were receiving direct federal grants to administer Migrant Even Start projects. These projects provide migrant families with an integrated program of early childhood education, adult education, and parenting education. As part of the national evaluation of the Even Start Family Literacy Program,…

Demonstration, Developmental and Research Project for Programs, Materials, Facilities and Educational Technology for Undereducated Adults: Ohio State Module. ABE Life-Centered Curriculum Development and Teaching Technique. Final Report.

ERIC Educational Resources Information Center

Morehead State Univ., KY.

Three types of instruction were used in the Ohio Module Project: traditional classes, programmed learning centers, and home instruction. Four major objectives of the project are: (1) to determine the kind of training program necessary to prepare paraprofessionals to operate an instructional program utilizing programmed materials, (2) to compare…

"2+2" Articulated Health Occupations Project. Nursing Program. Second Year Final Report.

ERIC Educational Resources Information Center

Paris Independent School District, TX.

A project was conducted to develop a 2 + 2 articulated training program in health careers to link the last 2 years of secondary and the first 2 years of postsecondary training. During the second year of the secondary project, the first year of training was implemented and the model program was further developed and refined. Project tasks included…

48 CFR 301.607 - Certification of Program and Project Managers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Certification of Program and Project Managers. 301.607 Section 301.607 Federal Acquisition Regulations System HEALTH AND HUMAN... Responsibilities 301.607 Certification of Program and Project Managers. ...

Use of a structured template to facilitate practice-based learning and improvement projects.

PubMed

McClain, Elizabeth K; Babbott, Stewart F; Tsue, Terance T; Girod, Douglas A; Clements, Debora; Gilmer, Lisa; Persons, Diane; Unruh, Greg

2012-06-01

The Accreditation Council for Graduate Medical Education (ACGME) requires residency programs to meet and demonstrate outcomes across 6 competencies. Measuring residents' competency in practice-based learning and improvement (PBLI) is particularly challenging. We developed an educational tool to meet ACGME requirements for PBLI. The PBLI template helped programs document quality improvement (QI) projects and supported increased scholarly activity surrounding PBLI learning. We reviewed program requirements for 43 residency and fellowship programs and identified specific PBLI requirements for QI activities. We also examined ACGME Program Information Form responses on PBLI core competency questions surrounding QI projects for program sites visited in 2008-2009. Data were integrated by a multidisciplinary committee to develop a peer-protected PBLI template guiding programs through process, documentation, and evaluation of QI projects. All steps were reviewed and approved through our GME Committee structure. An electronic template, companion checklist, and evaluation form were developed using identified project characteristics to guide programs through the PBLI process and facilitate documentation and evaluation of the process. During a 24 month period, 27 programs have completed PBLI projects, and 15 have reviewed the template with their education committees, but have not initiated projects using the template. The development of the tool generated program leaders' support because the tool enhanced the ability to meet program-specific objectives. The peer-protected status of this document for confidentiality and from discovery has been beneficial for program usage. The document aggregates data on PBLI and QI initiatives, offers opportunities to increase scholarship in QI, and meets the ACGME goal of linking measures to outcomes important to meeting accreditation requirements at the program and institutional level.

Use of a Structured Template to Facilitate Practice-Based Learning and Improvement Projects

PubMed Central

McClain, Elizabeth K.; Babbott, Stewart F.; Tsue, Terance T.; Girod, Douglas A.; Clements, Debora; Gilmer, Lisa; Persons, Diane; Unruh, Greg

2012-01-01

Background The Accreditation Council for Graduate Medical Education (ACGME) requires residency programs to meet and demonstrate outcomes across 6 competencies. Measuring residents' competency in practice-based learning and improvement (PBLI) is particularly challenging. Purpose We developed an educational tool to meet ACGME requirements for PBLI. The PBLI template helped programs document quality improvement (QI) projects and supported increased scholarly activity surrounding PBLI learning. Methods We reviewed program requirements for 43 residency and fellowship programs and identified specific PBLI requirements for QI activities. We also examined ACGME Program Information Form responses on PBLI core competency questions surrounding QI projects for program sites visited in 2008–2009. Data were integrated by a multidisciplinary committee to develop a peer-protected PBLI template guiding programs through process, documentation, and evaluation of QI projects. All steps were reviewed and approved through our GME Committee structure. Results An electronic template, companion checklist, and evaluation form were developed using identified project characteristics to guide programs through the PBLI process and facilitate documentation and evaluation of the process. During a 24 month period, 27 programs have completed PBLI projects, and 15 have reviewed the template with their education committees, but have not initiated projects using the template. Discussion The development of the tool generated program leaders' support because the tool enhanced the ability to meet program-specific objectives. The peer-protected status of this document for confidentiality and from discovery has been beneficial for program usage. The document aggregates data on PBLI and QI initiatives, offers opportunities to increase scholarship in QI, and meets the ACGME goal of linking measures to outcomes important to meeting accreditation requirements at the program and institutional level. PMID:23730444

Optical projects in the Clinic program at Harvey Mudd College

NASA Astrophysics Data System (ADS)

Yang, Q.

2017-08-01

Clinic program is the senior capstone program at Harvey Mudd College (HMC). Multidisciplinary and industry-sponsored projects allow a team of students to solve a real-world problem over one academic year. Over its 50 plus years, Clinic program has completed numerous optics related projects. This report gives an overview of the Clinic program, reviews recent optical projects and discusses how this program supports the learning of the HMC engineering students. A few sample optical projects with more details are presented to provide an insight of what challenges that undergraduates can overcome. Students achieve learning within the optics discipline and the related engineering disciplines. The experiences in these optical projects indicate the great potential to bringing optical hands-on projects into the undergraduate level. Because of the general engineering curriculum at HMC, these projects often work the best with a multidisciplinary nature even if the core of the project is optically focused. Students gain leadership training, oral and written communication skills and experiences in team work. Close relationship with the sponsor liaisons allows for the students to gain skills in professional conduct, management of tight schedule and a specified budget, and it well prepares the students to their engineering practice. Optical projects have their own sets of specific challenges, so it needs to be chosen properly to match the undergraduate skill sets such as those of HMC engineering students.

Worker Dislocation. Case Studies of Causes and Cures.

ERIC Educational Resources Information Center

Cook, Robert F., Ed.

Case studies were made of the following dislocated worker programs: Cummins Engine Company Dislocated Worker Project; GM-UAW Metropolitan Pontiac Retraining and Employment Program; Minnesota Iron Range Dislocated Worker Project; Missouri Dislocated Worker Program Job Search Assistance, Inc.; Hillsborough, North Carolina, Dislocated Worker Project;…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Osborn, C.S.; Osborn, D.E.

Through a small grant from the Center for Renewable Resources and a matching grant from the Arizona Solar Energy Commission, the Arizona Solar Energy Association has produced a state-wide catalogue of model solar projects. This catalogue presents some of the best solar and conservation projects in the state. It includes solar buildings, educational programs, community development programs, agricultural and industrial projects, state and legislative efforts, and commercial and business programs. Project selection was based on five main considerations: (1) cost-effectiveness, (2) valuable use of resources, (3) generation of jobs and transfer of skills, (4) replicability, and (5) scope. Shorter descriptionsmore » of significant projects not meeting the selection criteria were also included. The development of the catalogue program, its use and impact as a networking tool and the development and implementation of a regular updating program are described. The success of this type of program on information exchange, public education, and cross fertilization are explored. Special emphasis projects from the catalogue are also described.« less

Project Awareness: A Multi-State Leadership Project Addressing Sex Discrimination Issues in Education. A Training Program.

ERIC Educational Resources Information Center

Adelberger, Audra; And Others

This report describes a training program designed to increase educators' awareness of sex bias and its consequences in education. A major objective of the program is to suggest strategies for increasing educational opportunities for girls and women. The training program was part of a two-year project undertaken by seven state education agencies…

Programming your way out of the past: ISIS and the META Project

NASA Technical Reports Server (NTRS)

Birman, Kenneth P.; Marzullo, Keith

1989-01-01

The ISIS distributed programming system and the META Project are described. The ISIS programming toolkit is an aid to low-level programming that makes it easy to build fault-tolerant distributed applications that exploit replication and concurrent execution. The META Project is reexamining high-level mechanisms such as the filesystem, shell language, and administration tools in distributed systems.

Educator's Guide to Program Development in Natural Resources: Program Development Summary.

ERIC Educational Resources Information Center

Yoder, Jon; Maine, Neal

2001-01-01

Distinguishes between natural resource programs and natural resource projects and provides a project planning outline. Addresses critical elements and concerns in the development of natural resource programs. (DDR)

Virtual Special Issue on Catalysis at the U.S. Department of Energy’s National Laboratories

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pruski, Marek; Sadow, Aaron D.; Slowing, Igor I.

Catalysis research at the U.S. Department of Energy’s (DOE’s) National Laboratories covers a wide range of research topics in heterogeneous catalysis, homogeneous/molecular catalysis, biocatalysis, electrocatalysis, and surface science. Since much of the work at National Laboratories is funded by DOE, the research is largely focused on addressing DOE’s mission to ensure America’s security and prosperity by addressing its energy, environmental, and nuclear challenges through transformative science and technology solutions. The catalysis research carried out at the DOE National Laboratories ranges from very fundamental catalysis science, funded by DOE’s Office of Basic Energy Sciences (BES), to applied research and development (R&D)more » in areas such as biomass conversion to fuels and chemicals, fuel cells, and vehicle emission control with primary funding from DOE’s Office of Energy Efficiency and Renewable Energy. National Laboratories are home to many DOE Office of Science national scientific user facilities that provide researchers with the most advanced tools of modern science, including accelerators, colliders, supercomputers, light sources, and neutron sources, as well as facilities for studying the nanoworld and the terrestrial environment. National Laboratory research programs typically feature teams of researchers working closely together, often joining scientists from different disciplines to tackle scientific and technical problems using a variety of tools and techniques available at the DOE national scientific user facilities. Along with collaboration between National Laboratory scientists, interactions with university colleagues are common in National Laboratory catalysis R&D. In some cases, scientists have joint appointments at a university and a National Laboratory.« less

Focus on sustainability.

PubMed

Thompson, R J; Godiksen, L; Hansen, G; Gustafson, D J; Brinkerhoff, D W; Ingle, M D; Rounds, T; Wing, H

1990-01-01

In recent years, sustainability has become one of the most critical concepts in international development and is having a dramatic impact on the way development is conceptualized and carried out. The US Agency for International Development (USAID) is incorporating this concept into its programs and projects. Factors encouraging sustainability of projects and programs include host government policies that support or constrain program objectives, national and/or local commitment to project goals, managerial leadership that helps shape improved policies, collaboration at all staff levels in program management, financial resources that cover program operational costs, appropriate program technology, integration of the program with the social and cultural setting of the country, community involvement in the program, sound environmental management, technical assistance oriented to transferring skills and increasing institutional capacity, perception by the host country that the project is "effective," training provided by the project to transfer skill needed for capacity-building, integration of the program into existing institutional framework, and external political, economic and environmental factors. Impediments to sustainability are often inherent in the donor agency's programming process. This includes the implicit assumption that program objectives can be accomplished in a relatively short time frame, when in fact capacity-building requires a lengthy commitment. USAID professionals are pressured to show near-term results which emphasize outputs rather than purpose and goal-level accomplishments achievable only after extensive effort. The emphasis on obligating money and on the project paper as a sales document leads project designers to talk with a great deal more certainty about project results than is warranted by the complex development situation. Uncertainty and flexibility should be designed into projects so activities and objects can change as more information and on-site experience is gained. Instead of outputs, success should be measured in processes that will continue to produce long-term results. Emphasis should be placed on establishing policymaking processes and decision making procedures in the recipient country that will lead to sound economic policymaking on a continuing basis. Sustainable efforts in agriculture, health, rural development and their evaluation are examined for several USAID projects.

The Impact of a Dual-Project Action Learning Program: A Case of a Large IT Manufacturing Company in South Korea

ERIC Educational Resources Information Center

Yoon, Hyung Joon; Cho, Yonjoo; Bong, Hyeon-Cheol

2012-01-01

The primary purpose of this article is to evaluate the impact of a dual-project action learning program (DPALP) conducted in South Korea. A dual-project program requires each participant to carry out both team and individual projects. Cho and Egan's [2009. Action learning research: A systematic review and conceptual framework. "Human Resource…

Issues in NASA program and project management

NASA Technical Reports Server (NTRS)

Hoffman, Edward J. (Editor)

1994-01-01

This volume is the eighth in an ongoing series addressing current topics and lessons learned in NASA program and project management. Articles in this volume cover the following topics: (1) power sources for the Galileo and Ulysses Missions; (2) managing requirements; (3) program control of the Tropical Rainfall Measuring Mission; (4) project management method; (5) career development for project managers; and (6) resources for NASA managers.

Lessons Learned from FIPSE Projects II.

ERIC Educational Resources Information Center

Marcus, Dora; And Others

This monograph describes 30 college and university programs funded by the Fund for the Improvement of Postsecondary Education from 1989 to 1991. Each description includes information on program purpose, project activities, major insights and lessons, project continuation, and available information. The first group of 10 are programs focused on…

PROGRAMS TO UPGRADE CULTURALLY DEPRIVED YOUTH IN NEW YORK CITY.

ERIC Educational Resources Information Center

KARPAS, MELVIN R.

THE TWO PROGRAMS REVIEWED ARE THE DEMONSTRATION GUIDANCE PROJECT AND HIGHER HORIZONS PROGRAM. CHARACTERISTICS OF THE DEMONSTRATION GUIDANCE PROJECT INCLUDED EXTRA TEACHERS, SPECIAL TUTORING, GROUP AND INDIVIDUAL GUIDANCE AND COUNSELING, CLINICAL SERVICES, INTENSIVE COURSES IN ENGLISH, CULTURAL, AND ARTISTIC EVENTS. THE PROJECT STARTED WITH JUNIOR…

76 FR 15964 - Funding Priorities: Disability and Rehabilitation Research Projects and Centers Program

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-22

... past five years, the DBTACs have published numerous journal articles, held a series of research... and Rehabilitation Research Projects and Centers Program AGENCY: Office of Special Education and... Disability and Rehabilitation Research Projects and Centers Program administered by the National Institute on...

34 CFR 642.12 - What activities may a project conduct?

Code of Federal Regulations, 2011 CFR

2011-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Types of... project conduct? A Training program project may include on-site training, on-line training, conferences, internships, seminars, workshops, and the publication of manuals designed to improve the operations of Federal...

34 CFR 642.12 - What activities may a project conduct?

Code of Federal Regulations, 2012 CFR

2012-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Types of... project conduct? A Training program project may include on-site training, on-line training, conferences, internships, seminars, workshops, and the publication of manuals designed to improve the operations of Federal...

34 CFR 642.12 - What activities may a project conduct?

Code of Federal Regulations, 2014 CFR

2014-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Types of... project conduct? A Training program project may include on-site training, on-line training, conferences, internships, seminars, workshops, and the publication of manuals designed to improve the operations of Federal...

34 CFR 642.12 - What activities may a project conduct?

Code of Federal Regulations, 2013 CFR

2013-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Types of... project conduct? A Training program project may include on-site training, on-line training, conferences, internships, seminars, workshops, and the publication of manuals designed to improve the operations of Federal...

Fourth Year Status Report. Computerized Training Systems Project. Project ABACUS.

DTIC Science & Technology

1976-08-01

in 7 9. PERFORMING ORGANIZATION NAME AND ADOMEN ,,, 10. PROGRAM ELEMENT. PROJECT , TASK US Army Tra ining Support Center A R E A S WORK UNIT NUMBERS...transp ired during the fourth year of Project ABACUS, the A rmy ’s program for the development of a Computerized Training System. It inc l udes a...have transpired durlnq the fourth year of Project ABACUS, the Army ’s program for the developmen t o~ aprototype Computer i zed Training System. It

Lunar-Ultraviolet Telescope Experiment (LUTE) integrated program plan

NASA Technical Reports Server (NTRS)

Smith, Janice F. (Compiler); Forrest, Larry

1993-01-01

A detailed Lunar Ultraviolet Telescope Experiment (LUTE) program plan representing major decisions and tasks leading to those decisions for program execution are presented. The purpose of this task was to develop an integrated plan of project activities for the LUTE project, and to display the plan as an integrated network that shows the project activities, all critical interfaces, and schedules. The integrated network will provide the project manager with a frame work for strategic planning and risk management throughout the life of the project.

Four Tools for Science Fair Success

ERIC Educational Resources Information Center

Smith, Sherry Weaver; Messmer, Barbara; Storm, Bill; Weaver, Cheryl

2007-01-01

These teacher-tested ideas will guide students in creating true inquiry-based projects. Two of the ideas, the Topic Selection Wizard and Science Project Timeline, are appropriate for all science fair programs, even new ones. For existing programs, the Black Box of Project Improvement and After-School Project Clinic improve project quality and…

Interdisciplinary Early Childhood Handicapped Personnel Training Project.

ERIC Educational Resources Information Center

Swartz, Stanley L.

The report describes the Western Illinois University 0-6 Interdisciplinary Early Childhood Handicapped Personnel Training Project (WIU 0-6 Project)--a model project designed to demonstrate innovative methods to fill personnel needs for early childhood handicapped programs. The project is a 2 semester program to train professional educators in the…

23 CFR 660.112 - Project development.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Project development. 660.112 Section 660.112 Highways... PROGRAMS (DIRECT FEDERAL) Forest Highways § 660.112 Project development. (a) Projects to be administered by... Program. Projects to be administered by a cooperator shall be developed in accordance with Federal-aid...

23 CFR 660.112 - Project development.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 23 Highways 1 2013-04-01 2013-04-01 false Project development. 660.112 Section 660.112 Highways... PROGRAMS (DIRECT FEDERAL) Forest Highways § 660.112 Project development. (a) Projects to be administered by... Program. Projects to be administered by a cooperator shall be developed in accordance with Federal-aid...

23 CFR 660.112 - Project development.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 23 Highways 1 2014-04-01 2014-04-01 false Project development. 660.112 Section 660.112 Highways... PROGRAMS (DIRECT FEDERAL) Forest Highways § 660.112 Project development. (a) Projects to be administered by... Program. Projects to be administered by a cooperator shall be developed in accordance with Federal-aid...

23 CFR 660.112 - Project development.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 23 Highways 1 2012-04-01 2012-04-01 false Project development. 660.112 Section 660.112 Highways... PROGRAMS (DIRECT FEDERAL) Forest Highways § 660.112 Project development. (a) Projects to be administered by... Program. Projects to be administered by a cooperator shall be developed in accordance with Federal-aid...

Photovoltaic Subcontract Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Surek, Thomas; Catalano, Anthony

1993-03-01

This report summarizes the fiscal year (FY) 1992 progress of the subcontracted photovoltaic (PV) research and development (R D) performed under the Photovoltaic Advanced Research and Development Project at the National Renewable Energy Laboratory (NREL)-formerly the Solar Energy Research Institute (SERI). The mission of the national PV program is to develop PV technology for large-scale generation of economically competitive electric power in the United States. The technical sections of the report cover the main areas of the subcontract program: the Crystalline Materials and Advanced Concepts project, the Polycrystalline Thin Films project, Amorphous Silicon Research project, the Photovoltaic Manufacturing Technology (PVMaT)more » project, PV Module and System Performance and Engineering project, and the PV Analysis and Applications Development project. Technical summaries of each of the subcontracted programs provide a discussion of approaches, major accomplishments in FY 1992, and future research directions.« less

Sharing success: State energy program special projects results

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

2000-03-15

The State Energy Program was created in 1996 by an act of Congress through the consolidation of the State Energy Conservation Program (SECP) and the Institutional Conservation Program (ICP). Formerly, SECP provided funding for a variety of energy efficiency and renewable energy projects, and ICP assisted schools and hospitals with technical analysis and installation of energy conservation measures. Through these programs, more than 8,000 specific State conservation projects have been implemented since 1983 and more than 69,000 buildings have been made more energy efficient since 1979. The Department of Energy's Office of Energy Efficiency and Renewable Energy recognized the valuemore » of delivering programs through the States and created Special Projects in 1996. This report is an overview of State Energy Program operations, strategic focus, activities and accomplishments.« less

Involvement of the US Department of Defense in Civilian Assistance, Part I: a quantitative description of the projects funded by the Overseas Humanitarian, Disaster, and Civic Aid Program.

PubMed

Bourdeaux, Margaret Ellis; Lawry, Lynn; Bonventre, Eugene V; Burkle, Frederick M

2010-03-01

To review the history and goals of the US Department of Defense's largest civilian assistance program, the Overseas Humanitarian, Disaster and Civic Aid Program and to describe the number, geographic regions, years, key words, countries, and types of projects carried out under this program since 2001. Using the program's central database, we reviewed all approved projects since 2001 and tabulated them by year, combatant command, country, and key word. We also reviewed the project descriptions of projects funded between January 1, 2006, and February 9, 2008, and examined how their activities varied by combatant command and year. Of the 5395 projects in the database, 2097 were funded. Projects took place in more than 90 countries, with Southern, Pacific, and Africa Command hosting the greatest number. The most common types of projects were school, health, disaster response, and water infrastructure construction, and disaster-response training. The "global war on terror" was the key word most frequently tagged to project descriptions. Project descriptions lacked stated goals as well as implementation and coordination strategies with potential partners, and did not report outcome or impact indicators. The geographic reach of the program is vast and projects take place in a wide variety of public sectors. Yet their security and civilian assistance value remains unclear given the lack of stated project goals, implementation strategies, or measures of effectiveness. To facilitate transparency and policy discussion, we recommend project proposals include hypotheses as to how they will enhance US security, their relevance to the public sector they address, and outcome and impact indicators that can assess their value and effectiveness.

Alternative Basic Comprehensive Program (Project A.B.C.) Special Alternative Instructional Program. Final Evaluation Report 1992-93. OREA Report.

ERIC Educational Resources Information Center

Augustin, Marc A.

The Alternative Basic Comprehension Program (Project A.B.C.) for bilingual high school students was a special alternative instructional program funded by Title VII for the third year at two high schools in the Bronx. In the year under review, Project A.B.C. served 260 students of limited English proficiency (LEP). Participating students received…

42 CFR 56.503 - Project elements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... SERVICES Grants for Planning and Developing Migrant Health Programs § 56.503 Project elements. A project for the planning and development of a migrant health program supported under this subpart must: (a... an assessment of need of the population proposed to be served by the migrant health program for the...

34 CFR 647.4 - What activities and services may a project provide?

Code of Federal Regulations, 2010 CFR

2010-07-01

... PROGRAM General § 647.4 What activities and services may a project provide? A McNair project may provide... activities designed to prepare participants for doctoral study. (d) Tutoring. (e) Academic counseling. (f... programs. (g) Mentoring programs involving faculty members or students at institutions of higher education...

34 CFR 387.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 2 2011-07-01 2010-07-01 true What types of projects are authorized under this program...) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION EXPERIMENTAL AND....10 What types of projects are authorized under this program? The Experimental and Innovative Training...

34 CFR 387.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 2 2014-07-01 2013-07-01 true What types of projects are authorized under this program...) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION EXPERIMENTAL AND....10 What types of projects are authorized under this program? The Experimental and Innovative Training...

49 CFR 613.300 - Coordination of Federal and federally assisted programs and projects.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Coordination of Federal and federally assisted programs and projects. 613.300 Section 613.300 Transportation Other Regulations Relating to Transportation... Coordination of Federal and Federally Assisted Programs and Projects § 613.300 Coordination of Federal and...

77 FR 19682 - Proposed Information Collection; Wolf Livestock Demonstration Project Grant Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-02

...-FF09E30000] Proposed Information Collection; Wolf Livestock Demonstration Project Grant Program AGENCY: Fish... Interior and the Secretary of Agriculture to develop a Wolf Livestock Demonstration Project Grant Program... of livestock loss due to predation by wolves; and Compensate livestock producers for livestock losses...

76 FR 793 - Multistate Conservation Grant Program; Priority List for Conservation Projects

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-06

...; 91400-9410-0000-7B] Multistate Conservation Grant Program; Priority List for Conservation Projects... 2011 priority list of wildlife and sport fish conservation projects from the Association of Fish and Wildlife Agencies (AFWA). As required by the Wildlife and Sport Fish Restoration Programs Improvement Act...

7 CFR 1219.50 - Budgets, programs, plans, and projects.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Budgets, programs, plans, and projects. 1219.50... Order Budgets, Expenses, and Assessments § 1219.50 Budgets, programs, plans, and projects. (a) The Board shall submit to the Secretary, on a fiscal period basis, annual budgets of its anticipated expenses and...

Annual Program: Library Services and Construction Act, 1987-1988.

ERIC Educational Resources Information Center

South Carolina State Library, Columbia.

This report presents the 1978-1988 annual Library Services and Construction Act (LSCA) program for the South Carolina State Library. This program includes fiscal information and project descriptions for the following LCSA projects under Title I-Library Services: (1) Projects IA-General Administration; (2) IB-Library Interpretation; (3) IIA-General…

NASA scientific and technical information program multimedia initiative

NASA Technical Reports Server (NTRS)

Cotter, Gladys A.; Kaye, Karen

1993-01-01

This paper relates the experiences of the NASA Scientific and Technical Information Program in introducing multimedia within the STI Program framework. A discussion of multimedia technology is included to provide context for the STI Program effort. The STI Program's Multimedia Initiative is discussed in detail. Parallels and differences between multimedia and traditional information systems project development are highlighted. Challenges faced by the program in initiating its multimedia project are summarized along with lessons learned. The paper concludes with a synopsis of the benefits the program hopes to provide its users through the introduction of multimedia illustrated by examples of successful multimedia projects.

STI Program Multimedia Initiative

NASA Technical Reports Server (NTRS)

Cotter, Gladys A.; Kaye, Karen

1993-01-01

This paper relates the experience of the NASA Scientific and Technical Information Program in introducing multimedia within the STI Program framework. A discussion of multimedia technology is included to provide context for the STI Program effort. The STI Program's Multimedia Initiative is discussed in detail. Parallels and differences between multimedia and traditional information systems project development are highlighted. Challenges faced by the program in initiating its multimedia project are summarized along with lessons learned. The paper concludes with a synopsis of the benefits the program hopes to provide its users through the introduction of multimedia illustrated by examples of successful multimedia projects.

SELENIUM TREATMENT/REMOVAL ALTERNATIVES DEMONSTRATION PROJECT - MINE WASTE TECHNOLOGY PROGRAM ACTIVITY III, PROJECT 20

EPA Science Inventory

This document is the final report for EPA's Mine WAste Technology Program (MWTP) Activity III, Project 20--Selenium Treatment/Removal Alternatives Demonstration project. Selenium contamination originates from many sources including mining operations, mineral processing, abandoned...

THE ATLANTA SUPERSITE PROJECT

EPA Science Inventory

The Atlanta Supersites project is the first of two Supersites projects to be established during Phase I of EPA's Supersites Program; Phase 11 is being established through a Request for Assistance. The other initial project is in Fresno, California. The Supersites Program is par...

Introduction.

ERIC Educational Resources Information Center

Fortner, Rosanne

1994-01-01

Provides an introduction to Project WET (Water Education for Teachers). Examines approaches to water education, how the Project WET program is different from other water education projects, how the program is implemented in the classroom, what kinds of materials are available, and how to obtain Project WET. (LZ)

Relating Women's Experience and Skills to Competencies Required in Selected Occupations and Vocational Education Programs. Project Report, Task C1, Revised Competency Analysis: Credentialing Women's Life Experiences (Project ACCESS).

ERIC Educational Resources Information Center

Ekstrom, Ruth B.; And Others

Competency scales were developed for 10 direct-entry occupations and 10 vocational education programs as part of Project ACCESS, a project to assess women's life experience learning for entrance into employment and for advanced placement in vocational education programs. Development of the occupational scales involved employer interviews, followed…

Issues in NASA program and project management

NASA Technical Reports Server (NTRS)

Hoban, Francis T. (Editor)

1992-01-01

This volume is the fifth in an ongoing series on aerospace project management at NASA. Articles in this volume cover: an overview of the project cycle; SE&I management for manned space flight programs; shared experiences from NASA Programs and Projects - 1975; cost control for Mariner Venus/Mercury 1973; and the Space Shuttle - a balancing of design and politics. A section on resources for NASA managers rounds out the publication.

7 CFR 2903.6 - Project types.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.6 Project types. OEPNU intends to award continuation grants to successful Biodiesel Fuel Education Program applicants. A continuation...

7 CFR 2903.6 - Project types.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.6 Project types. OEPNU intends to award continuation grants to successful Biodiesel Fuel Education Program applicants. A continuation...

7 CFR 2903.6 - Project types.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.6 Project types. OEPNU intends to award continuation grants to successful Biodiesel Fuel Education Program applicants. A continuation...

7 CFR 2903.6 - Project types.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.6 Project types. OEPNU intends to award continuation grants to successful Biodiesel Fuel Education Program applicants. A continuation...

7 CFR 2903.6 - Project types.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGRICULTURE BIODIESEL FUEL EDUCATION PROGRAM Program Description § 2903.6 Project types. OEPNU intends to award continuation grants to successful Biodiesel Fuel Education Program applicants. A continuation...

The TMDL Program Results Analysis Project: Matching Results Measures with Program Expectations

EPA Pesticide Factsheets

The paper provides a detailed description of the aims, methods and outputs of the program evaluation project undertaken by EPA in order to generate the insights needed to make TMDL program improvements.

Elective Program Projects

ERIC Educational Resources Information Center

Estrada, Christelle

1976-01-01

Outlined is an interdisciplinary program in Ecology and Oceanography for grades six through eight. Numerous student projects are suggested in the outline and the course requirements and the project system are explained. (MA)

Project EX: A Program of Empirical Research on Adolescent Tobacco Use Cessation

PubMed Central

Sussman, Steve; McCuller, William J; Zheng, Hong; Pfingston, Yvonne M; Miyano, James; Dent, Clyde W

2004-01-01

This paper presents the Project EX research program. The historical background for Project EX is presented, including a brief summary of reasons youth fail to quit tobacco use, the disappointing status of previous cessation research, and the teen cessation trial that provided the template for the current project (Project TNT). Next, program development studies for Project EX are described. Through use of focus groups, a theme study (concept evaluation of written activity descriptions), a component study, and pilot studies, an eight-session program was developed. This program involves novel activities (e.g., "talk show enactments," games, and alternative medicine-type activities such as yoga and meditation) in combination with motivation enhancement and cognitive-behavioral strategies to motivate and instruct in cessation initiation and maintenance efforts. The outcomes of the first experimental trial of Project EX, a school-based clinic program, are described, followed by a posthoc analysis of its effects mediation. A second EX study, a multiple baseline single group pilot study design in Wuhan, China, is described next. Description of a second experimental trial follows, which tested EX with nicotine gum versus a natural herb. A third experimental trial that tests a classroom prevention/cessation version of EX is then introduced. Finally, the implications of this work are discussed. The intent-to-treat quit rate for Project EX is approximately 15% across studies, double that of a standard care comparison. Effects last up to a six-month post-program at regular and alternative high schools. Through a systematic protocol of empirical program development and field trials, an effective and replicable model teen tobacco use cessation program is established. Future cessation work might expand on this work.

Project EX: A Program of Empirical Research on Adolescent Tobacco Use Cessation

PubMed Central

Sussman, Steve; McCuller, William J; Zheng, Hong; Pfingston, Yvonne M; Miyano, James; Dent, Clyde W

2004-01-01

This paper presents the Project EX research program. The historical background for Project EX is presented, including a brief summary of reasons youth fail to quit tobacco use, the disappointing status of previous cessation research, and the teen cessation trial that provided the template for the current project (Project TNT). Next, program development studies for Project EX are described. Through use of focus groups, a theme study (concept evaluation of written activity descriptions), a component study, and pilot studies, an eight-session program was developed. This program involves novel activities (e.g., "talk show enactments," games, and alternative medicine-type activities such as yoga and meditation) in combination with motivation enhancement and cognitive-behavioral strategies to motivate and instruct in cessation initiation and maintenance efforts. The outcomes of the first experimental trial of Project EX, a school-based clinic program, are described, followed by a posthoc analysis of its effects mediation. A second EX study, a multiple baseline single group pilot study design in Wuhan, China, is described next. Description of a second experimental trial follows, which tested EX with nicotine gum versus a natural herb. A third experimental trial that tests a classroom prevention/cessation version of EX is then introduced. Finally, the implications of this work are discussed. The intent-to-treat quit rate for Project EX is approximately 15% across studies, double that of a standard care comparison. Effects last up to a six-month post-program at regular and alternative high schools. Through a systematic protocol of empirical program development and field trials, an effective and replicable model teen tobacco use cessation program is established. Future cessation work might expand on this work. PMID:19570278

Columbia River Basin Fish and Wildlife Program Annual Implementation Work Plan for Fiscal Year 1991.

DOE Office of Scientific and Technical Information (OSTI.GOV)

United States. Bonneville Power Administration; Northwest Power Planning Council; Columbia Basin Fish and Wildlife Authority

1990-09-01

The Columbia River Basin Fish and Wildlife Program (Program) was developed by the Northwest Power Planning Council (Council) in accordance with Public Law 96-501, the Pacific Northwest Electric Power Planning and Conservation Act (Act). The purpose of the Program is to guide the Bonneville Power Administration (BPA) and other Federal agencies in carrying out their responsibilities to protect, mitigate, and enhance fish and wildlife of the Columbia River Basin. The Act explicitly gives BPA the authority and responsibility to use the BPA fund for these ends, to the extent that fish and wildlife are affected by the development and operationmore » of hydroelectric generation in the Columbia River Basin. The Columbia River Basin Fish and Wildlife Program Annual Implementation Work Plan (AIWP) presents BPA's draft plans for implementing the Program during Fiscal Year (FY) 1991. The AIWP reflects the primary goals of the Council's Action Plan (Section 1400 of the Program): to provide a solid, timely, and focused basis for budgeting and planning. In addition, the AIWP provides a means to judge the progress and the success of Program implementation. The AIWP is based on the outline developed by the Policy Review Group (PRG) during Step 1 of the annual cycle of the Implementation Planning Process (IPP), which is described in Section III. This AIWP has been organized and written to meet the specific needs of Program Items 10.1-10.3. The AIWP includes schedules with key milestones for 1 and beyond, and addresses the Action Items assigned to BPA in Section 1400 of the 1987 Program and in subsequent amendments. All Program projects discussed in the AIWP are listed in Tables 1 and 2 according to their status as of September 1, 1990. Table 1 (pp. 3-14) lists completed, ongoing, and deferred projects. Table 2 (pp. 15-17) lists FY 1991 new-start projects. ''Ongoing'' status indicates that the project started in FY 1990 or before and that it is expected to continue through part or all of FY 1991. ''Deferred'' means that BPA implementation has been postponed to FY 1992 or later. ''Completed'' indicates completion during FY 1990. ''New'' denotes projects planned for BPA implementation in FY 1991. However, several of these new projects were still under review by the Policy Review Group as the FY 1991 AIWP went to press. The new projects still under review have been noted in Table 2 and in the text of the AIWP. A number of projects are expected to begin in late FY 1990 and have been listed in Table 1 of the Draft AIWP as ''Projected FY '90 Starts,'' based on their projected start dates. Several other projects are expected to end in late FY 1990. These projects have been listed in Table 1 as ''Projected FY '90 Completions,'' based on their projected completion dates. Section VIII describes BPA's non-Program, internal support projects. These projects were not subject to review by the PRG and have been included in the AIWP to help the PRG and the public to better understand what BPA is doing.« less

NASA's Morphing Project Research Summaries in Fiscal Year 2002

NASA Technical Reports Server (NTRS)

McGowan, Anna-Maria R.; Waszak, Martin R.

2005-01-01

The Morphing Project at the National Aeronautics and Space Agency s (NASA) Langley Research Center (LaRC) is part of the Breakthrough Vehicle Technologies Project, Vehicle Systems Program that conducts fundamental research on advanced technologies for future flight vehicles. The objectives of the Morphing Project are to develop and assess the advanced technologies and integrated component concepts to enable efficient, multi-point adaptability of flight vehicles; primarily through the application of adaptive structures and adaptive flow control to substantially alter vehicle performance characteristics. This document is a compilation of research summaries and other information on the project for fiscal year 2002. The focus is to provide a brief overview of the project content, technical results and lessons learned. At the time of publication, the Vehicle Systems Program (which includes the Morphing Project) is undergoing a program re-planning and reorganization. Accordingly, the programmatic descriptions of this document pertain only to the program as of fiscal year 2002.

Photovoltaic Subcontract Program. Annual report, FY 1992

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1993-03-01

This report summarizes the fiscal year (FY) 1992 progress of the subcontracted photovoltaic (PV) research and development (R&D) performed under the Photovoltaic Advanced Research and Development Project at the National Renewable Energy Laboratory (NREL)-formerly the Solar Energy Research Institute (SERI). The mission of the national PV program is to develop PV technology for large-scale generation of economically competitive electric power in the United States. The technical sections of the report cover the main areas of the subcontract program: the Crystalline Materials and Advanced Concepts project, the Polycrystalline Thin Films project, Amorphous Silicon Research project, the Photovoltaic Manufacturing Technology (PVMaT) project,more » PV Module and System Performance and Engineering project, and the PV Analysis and Applications Development project. Technical summaries of each of the subcontracted programs provide a discussion of approaches, major accomplishments in FY 1992, and future research directions.« less

7 CFR 1207.311 - Programs and projects.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan Definitions § 1207.311 Programs and projects. Programs and...

7 CFR 1207.311 - Programs and projects.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan Definitions § 1207.311 Programs and projects. Programs and...

7 CFR 1207.311 - Programs and projects.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan Definitions § 1207.311 Programs and projects. Programs and...

7 CFR 1207.311 - Programs and projects.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE POTATO RESEARCH AND PROMOTION PLAN Potato Research and Promotion Plan Definitions § 1207.311 Programs and projects. Programs and...

Visualizing the Complex Process for Deep Learning with an Authentic Programming Project

ERIC Educational Resources Information Center

Peng, Jun; Wang, Minhong; Sampson, Demetrios

2017-01-01

Project-based learning (PjBL) has been increasingly used to connect abstract knowledge and authentic tasks in educational practice, including computer programming education. Despite its promising effects on improving learning in multiple aspects, PjBL remains a struggle due to its complexity. Completing an authentic programming project involves a…

National Institute Of Education Project for Pre-Doctoral Fellows at the University of Tennessee, Knoxville.

ERIC Educational Resources Information Center

Banta, Trudy W.; And Others

A formative evaluation of a University of Tennessee, Knoxville, predoctoral project was conducted. The program, which was funded by the federal Minorities and Women's Program, was designed to increase the participation of minorities and women in educational research and development. The project, "Experimental Program for Opportunities in…

Educational Program Development for the Rural Disadvantaged. Research Report.

ERIC Educational Resources Information Center

Thomas, Hollie B.; And Others

As part of a far-reaching research project on educational needs of the rural disadvantaged, this phase of the Rural Education Disadvantaged Youth Project (Project REDY) dealt with the development and evaluation of a vocationally-oriented family-centered educational program. The model program, which was field-tested at a single site, focused upon:…

Project Citizen: Students Practice Democratic Principles While Conducting Community Projects

ERIC Educational Resources Information Center

Medina-Jerez, William; Bryant, Carol; Green, Carie

2010-01-01

Project Citizen is a program sponsored by the U.S. Department of Education's congressionally funded Center for Civic Education, which sponsors both domestic and international programs. The Center for Civic Education's Civitas International Programs pair U.S. states with countries around the world based on a variety of factors; including geographic…

42 CFR 51a.1 - To which programs does this regulation apply?

Code of Federal Regulations, 2014 CFR

2014-10-01

... (CISS) projects for the development and expansion of: maternal and infant health home visiting; projects... GRANTS FOR MATERNAL AND CHILD HEALTH § 51a.1 To which programs does this regulation apply? The regulation... Child Health (MCH) Federal Set-Aside project grant programs. Section 502(a) authorizes funding for...

42 CFR 51a.1 - To which programs does this regulation apply?

Code of Federal Regulations, 2012 CFR

2012-10-01

... (CISS) projects for the development and expansion of: maternal and infant health home visiting; projects... GRANTS FOR MATERNAL AND CHILD HEALTH § 51a.1 To which programs does this regulation apply? The regulation... Child Health (MCH) Federal Set-Aside project grant programs. Section 502(a) authorizes funding for...

42 CFR 51a.1 - To which programs does this regulation apply?

Code of Federal Regulations, 2013 CFR

2013-10-01

... (CISS) projects for the development and expansion of: maternal and infant health home visiting; projects... GRANTS FOR MATERNAL AND CHILD HEALTH § 51a.1 To which programs does this regulation apply? The regulation... Child Health (MCH) Federal Set-Aside project grant programs. Section 502(a) authorizes funding for...

42 CFR 51a.1 - To which programs does this regulation apply?

Code of Federal Regulations, 2011 CFR

2011-10-01

... (CISS) projects for the development and expansion of: maternal and infant health home visiting; projects... GRANTS FOR MATERNAL AND CHILD HEALTH § 51a.1 To which programs does this regulation apply? The regulation... Child Health (MCH) Federal Set-Aside project grant programs. Section 502(a) authorizes funding for...

42 CFR 51a.1 - To which programs does this regulation apply?

Code of Federal Regulations, 2010 CFR

2010-10-01

... (CISS) projects for the development and expansion of: maternal and infant health home visiting; projects... GRANTS FOR MATERNAL AND CHILD HEALTH § 51a.1 To which programs does this regulation apply? The regulation... Child Health (MCH) Federal Set-Aside project grant programs. Section 502(a) authorizes funding for...

Assigning a Thesis Project In the Two-Year Architectural Technology Program

ERIC Educational Resources Information Center

Obermeyer, Thomas

1977-01-01

The "thesis project" assigned in the sixth quarter of the eight-quarter architectural technology program at the Dakota County Area Vocational-Technical Institute in Rosemont, Minnesota, requires the students to design a building for a local public service organization or government agency. The complete project will include a program, a…

42 CFR 137.301 - How are project and program environmental review costs identified?

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false How are project and program environmental review... HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Construction Nepa Process § 137.301 How are project and program environmental review costs identified? (a) The...

42 CFR 137.301 - How are project and program environmental review costs identified?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false How are project and program environmental review... HUMAN SERVICES INDIAN HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES TRIBAL SELF-GOVERNANCE Construction Nepa Process § 137.301 How are project and program environmental review costs identified? (a) The...

Project "School:" A Handbook of a Validated Developmental Dropout Prevention Program's Methods and Procedures.

ERIC Educational Resources Information Center

Hooban, Louis; Pugsley, Robert

Project SCHOOL (School Concerned with Helping Others' Objectives and Learning) is a program developed to prevent dropping out through group counseling, parent counseling, and positive reinforcement. Project SCHOOL operates as a dropout prevention program in grades 1-12. Teachers and counselors, using a locally developed check list and other…

78 FR 25093 - Multistate Conservation Grant Program; Priority List and Approval for Conservation Projects

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-29

... Fish Restoration Act (16 U.S.C. 777 et seq.) and established the Multistate Conservation Grant Program...-9410-0000] Multistate Conservation Grant Program; Priority List and Approval for Conservation Projects... year 2013 priority list of wildlife and sport fish conservation projects from the Association of Fish...

7 CFR 3430.1004 - Project types and priorities.

Code of Federal Regulations, 2014 CFR

2014-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1004 Project types and priorities. (a) Project types. The Sun... on the basis of merit, quality, and relevance to advancing the purposes of the Sun Grant Program. (2... provided by NIFA for the Sun Grant Program that are not allocated to the Subcenter. For the Subcenter, the...

7 CFR 3430.1004 - Project types and priorities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1004 Project types and priorities. (a) Project types. The Sun... on the basis of merit, quality, and relevance to advancing the purposes of the Sun Grant Program. (2... provided by NIFA for the Sun Grant Program that are not allocated to the Subcenter. For the Subcenter, the...

7 CFR 3430.1004 - Project types and priorities.

Code of Federal Regulations, 2013 CFR

2013-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1004 Project types and priorities. (a) Project types. The Sun... on the basis of merit, quality, and relevance to advancing the purposes of the Sun Grant Program. (2... provided by NIFA for the Sun Grant Program that are not allocated to the Subcenter. For the Subcenter, the...

7 CFR 3430.1004 - Project types and priorities.

Code of Federal Regulations, 2012 CFR

2012-01-01

... ADMINISTRATIVE PROVISIONS Sun Grant Program § 3430.1004 Project types and priorities. (a) Project types. The Sun... on the basis of merit, quality, and relevance to advancing the purposes of the Sun Grant Program. (2... provided by NIFA for the Sun Grant Program that are not allocated to the Subcenter. For the Subcenter, the...

Program Assistance for Neophytes. Project PAN, 1988-89. OREA Evaluation Section Report.

ERIC Educational Resources Information Center

Berney, Tomi D.; Hriskos, Constantine

In its fourth year, Program Assistance for Neophytes (Project PAN) served 455 students at 2 high schools. The program provided support services and supplemental instruction to students of limited English proficiency (LEP) as well as English-proficient (EP) students. The project provided LEP students with a transitional period of bilingual…

Implementing a Project-Based Learning Model in a Pre-Service Leadership Program

ERIC Educational Resources Information Center

Albritton, Shelly; Stacks, Jamie

2016-01-01

This paper describes two instructors' efforts to more authentically engage students in a preservice leadership program's course called Program Planning and Evaluation by using a project-based learning approach. Markham, Larmer, and Ravitz (2003) describe project-based learning (PjBL) as "a systematic teaching method that engages students in…

34 CFR 425.1 - What is the Demonstration Projects for the Integration of Vocational and Academic Learning Program?

Code of Federal Regulations, 2013 CFR

2013-07-01

... of Vocational and Academic Learning Program? 425.1 Section 425.1 Education Regulations of the Offices... EDUCATION DEMONSTRATION PROJECTS FOR THE INTEGRATION OF VOCATIONAL AND ACADEMIC LEARNING PROGRAM General § 425.1 What is the Demonstration Projects for the Integration of Vocational and Academic Learning...

34 CFR 425.1 - What is the Demonstration Projects for the Integration of Vocational and Academic Learning Program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... of Vocational and Academic Learning Program? 425.1 Section 425.1 Education Regulations of the Offices... EDUCATION DEMONSTRATION PROJECTS FOR THE INTEGRATION OF VOCATIONAL AND ACADEMIC LEARNING PROGRAM General § 425.1 What is the Demonstration Projects for the Integration of Vocational and Academic Learning...

34 CFR 425.1 - What is the Demonstration Projects for the Integration of Vocational and Academic Learning Program?

Code of Federal Regulations, 2012 CFR

2012-07-01

... of Vocational and Academic Learning Program? 425.1 Section 425.1 Education Regulations of the Offices... EDUCATION DEMONSTRATION PROJECTS FOR THE INTEGRATION OF VOCATIONAL AND ACADEMIC LEARNING PROGRAM General § 425.1 What is the Demonstration Projects for the Integration of Vocational and Academic Learning...

34 CFR 425.1 - What is the Demonstration Projects for the Integration of Vocational and Academic Learning Program?

Code of Federal Regulations, 2014 CFR

2014-07-01

... of Vocational and Academic Learning Program? 425.1 Section 425.1 Education Regulations of the Offices... EDUCATION DEMONSTRATION PROJECTS FOR THE INTEGRATION OF VOCATIONAL AND ACADEMIC LEARNING PROGRAM General § 425.1 What is the Demonstration Projects for the Integration of Vocational and Academic Learning...

Undergraduate computational physics projects on quantum computing

NASA Astrophysics Data System (ADS)

Candela, D.

2015-08-01

Computational projects on quantum computing suitable for students in a junior-level quantum mechanics course are described. In these projects students write their own programs to simulate quantum computers. Knowledge is assumed of introductory quantum mechanics through the properties of spin 1/2. Initial, more easily programmed projects treat the basics of quantum computation, quantum gates, and Grover's quantum search algorithm. These are followed by more advanced projects to increase the number of qubits and implement Shor's quantum factoring algorithm. The projects can be run on a typical laptop or desktop computer, using most programming languages. Supplementing resources available elsewhere, the projects are presented here in a self-contained format especially suitable for a short computational module for physics students.

Initiating the 2002 Mars Science Laboratory (MSL) Technology Program

NASA Technical Reports Server (NTRS)

Caffrey, Robert T.; Udomkesmalee, Gabriel; Hayati, Samad A.; Henderson, Rebecca

2004-01-01

The Mars Science Laboratory (MSL) Project is an aggressive mission launching in 2009 to investigate the Martian environment and requires new capabilities that are currently are not available. The MSL Technology Program is developing a wide-range of technologies needed for this Mission and potentially other space missions. The MSL Technology Program reports to both the MSL Project and the Mars Technology Program (MTP). The dual reporting process creates a challenging management situation, but ensures the new technology meets both the specific MSL requirements and the broader Mars Program requirements. MTP is a NASA-wide technology development program managed by JPL and is divided into a Focused Program and a Base Program. The MSL Technology Program is under the focused program and is tightly coupled to MSL's mission milestones and deliverables. The technology budget is separate from the flight Project budget, but the technology's requirements and the development process are tightly coordinated with the Project. The MSL Technology Program combines the proven management techniques of flight projects with the commercial technology management strategies of industry and academia, to create a technology management program that meets the short-term requirements of MSL and the long-term requirements of MTP. This paper examines the initiation of 2002 MSL Technology program. Some of the areas discussed in this paper include technology definition, task selection, technology management, and technology assessment. This paper also provides an update of the 2003 MSL technology program and examines some of the drivers that changed the program from its initiation.

Distance Learning Programming.

ERIC Educational Resources Information Center

Lucero, Jesus Ricardo; And Others

This resource guide for distance learning information, courses, and programming covers: (1) audiographics programming by the Pennsylvania Teleteaching Project; (2) cable programming, including the Cable Alliance for Education's Cable in the Classroom projects, Consumer News and Business Channel, Nostalgia Television, PENNARAMA Channel, Silent…

Leveraging Independent Management and Chief Engineer Hierarchy: Vertically and Horizontally-Derived Technical Authority Value

NASA Technical Reports Server (NTRS)

Barley, Bryan; Newhouse, Marilyn

2012-01-01

In the development of complex spacecraft missions, project management authority is usually extended hierarchically from NASA's highest agency levels down to the implementing institution's project team level, through both the center and the program. In parallel with management authority, NASA utilizes a complementary, but independent, hierarchy of technical authority (TA) that extends from the agency level to the project, again, through both the center and the program. The chief engineers (CEs) who serve in this technical authority capacity oversee and report on the technical status and ensure sound engineering practices, controls, and management of the projects and programs. At the lowest level, implementing institutions assign project CEs to technically engage projects, lead development teams, and ensure sound technical principles, processes, and issue resolution. At the middle level, programs and centers independently use CEs to ensure the technical success of their projects and programs. At the agency level, NASA's mission directorate CEs maintain technical cognizance over every program and project in their directorate and advise directorate management on the technical, cost, schedule, and programmatic health of each. As part of this vertically-extended CE team, a program level CE manages a continually varying balance between penetration depth and breadth across his or her assigned missions. Teamwork issues and information integration become critical for management at all levels to ensure value-added use of both the synergy available between CEs at the various agency levels, and the independence of the technical authority at each organization.

Advances in the NASA Earth Science Division Applied Science Program

NASA Astrophysics Data System (ADS)

Friedl, L.; Bonniksen, C. K.; Escobar, V. M.

2016-12-01

The NASA Earth Science Division's Applied Science Program advances the understanding of and ability to used remote sensing data in support of socio-economic needs. The integration of socio-economic considerations in to NASA Earth Science projects has advanced significantly. The large variety of acquisition methods used has required innovative implementation options. The integration of application themes and the implementation of application science activities in flight project is continuing to evolve. The creation of the recently released Earth Science Division, Directive on Project Applications Program and the addition of an application science requirement in the recent EVM-2 solicitation document NASA's current intent. Continuing improvement in the Earth Science Applications Science Program are expected in the areas of thematic integration, Project Applications Program tailoring for Class D missions and transfer of knowledge between scientists and projects.

National Workplace Literacy Program. 1993 Abstracts.

ERIC Educational Resources Information Center

Office of Vocational and Adult Education (ED), Washington, DC. National Workplace Literacy Program.

This publication presents the abstracts of the 57 National Workplace Literacy Program 1993 projects. Each abstract provides the following information: project title; award number; project director; awardee; address; telephone and fax numbers; funds by fiscal year (federal and nonfederal); award period; federal project officer; objectives;…

7 CFR 210.28 - Pilot project exemptions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 4 2011-01-01 2011-01-01 false Pilot project exemptions. 210.28 Section 210.28... AGRICULTURE CHILD NUTRITION PROGRAMS NATIONAL SCHOOL LUNCH PROGRAM Additional Provisions § 210.28 Pilot project exemptions. Those State agencies or school food authorities selected for the pilot projects...

7 CFR 210.28 - Pilot project exemptions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 4 2010-01-01 2010-01-01 false Pilot project exemptions. 210.28 Section 210.28... AGRICULTURE CHILD NUTRITION PROGRAMS NATIONAL SCHOOL LUNCH PROGRAM Additional Provisions § 210.28 Pilot project exemptions. Those State agencies or school food authorities selected for the pilot projects...

7 CFR 1944.529 - Project selection.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 13 2012-01-01 2012-01-01 false Project selection. 1944.529 Section 1944.529...) PROGRAM REGULATIONS (CONTINUED) HOUSING Technical and Supervisory Assistance Grants § 1944.529 Project selection. (a) Projects must meet the following criteria: (1) Provide a program of supervisory assistance as...

7 CFR 1944.529 - Project selection.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 13 2014-01-01 2013-01-01 true Project selection. 1944.529 Section 1944.529...) PROGRAM REGULATIONS (CONTINUED) HOUSING Technical and Supervisory Assistance Grants § 1944.529 Project selection. (a) Projects must meet the following criteria: (1) Provide a program of supervisory assistance as...

7 CFR 1944.529 - Project selection.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 13 2013-01-01 2013-01-01 false Project selection. 1944.529 Section 1944.529...) PROGRAM REGULATIONS (CONTINUED) HOUSING Technical and Supervisory Assistance Grants § 1944.529 Project selection. (a) Projects must meet the following criteria: (1) Provide a program of supervisory assistance as...

7 CFR 1944.529 - Project selection.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 13 2011-01-01 2009-01-01 true Project selection. 1944.529 Section 1944.529...) PROGRAM REGULATIONS (CONTINUED) HOUSING Technical and Supervisory Assistance Grants § 1944.529 Project selection. (a) Projects must meet the following criteria: (1) Provide a program of supervisory assistance as...

Disease management projects and the Chronic Care Model in action: baseline qualitative research.

PubMed

Walters, Bethany Hipple; Adams, Samantha A; Nieboer, Anna P; Bal, Roland

2012-05-11

Disease management programs, especially those based on the Chronic Care Model (CCM), are increasingly common in The Netherlands. While disease management programs have been well-researched quantitatively and economically, less qualitative research has been done. The overall aim of the study is to explore how disease management programs are implemented within primary care settings in The Netherlands; this paper focuses on the early development and implementation stages of five disease management programs in the primary care setting, based on interviews with project leadership teams. Eleven semi-structured interviews were conducted at the five selected sites with sixteen professionals interviewed; all project directors and managers were interviewed. The interviews focused on each project's chosen chronic illness (diabetes, eating disorders, COPD, multi-morbidity, CVRM) and project plan, barriers to development and implementation, the project leaders' action and reactions, as well as their roles and responsibilities, and disease management strategies. Analysis was inductive and interpretive, based on the content of the interviews. After analysis, the results of this research on disease management programs and the Chronic Care Model are viewed from a traveling technology framework. This analysis uncovered four themes that can be mapped to disease management and the Chronic Care Model: (1) changing the health care system, (2) patient-centered care, (3) technological systems and barriers, and (4) integrating projects into the larger system. Project leaders discussed the paths, both direct and indirect, for transforming the health care system to one that addresses chronic illness. Patient-centered care was highlighted as needed and a paradigm shift for many. Challenges with technological systems were pervasive. Project leaders managed the expenses of a traveling technology, including the social, financial, and administration involved. At the sites, project leaders served as travel guides, assisting and overseeing the programs as they traveled from the global plans to local actions. Project leaders, while hypothetically in control of the programs, in fact shared control of the traveling of the programs with patients, clinicians, and outside consultants. From this work, we can learn what roadblocks and expenses occur while a technology travels, from a project leader's point of view.

Better than Nature: Nicotinamide Biomimetics That Outperform Natural Coenzymes.

PubMed

Knaus, Tanja; Paul, Caroline E; Levy, Colin W; de Vries, Simon; Mutti, Francesco G; Hollmann, Frank; Scrutton, Nigel S

2016-01-27

The search for affordable, green biocatalytic processes is a challenge for chemicals manufacture. Redox biotransformations are potentially attractive, but they rely on unstable and expensive nicotinamide coenzymes that have prevented their widespread exploitation. Stoichiometric use of natural coenzymes is not viable economically, and the instability of these molecules hinders catalytic processes that employ coenzyme recycling. Here, we investigate the efficiency of man-made synthetic biomimetics of the natural coenzymes NAD(P)H in redox biocatalysis. Extensive studies with a range of oxidoreductases belonging to the "ene" reductase family show that these biomimetics are excellent analogues of the natural coenzymes, revealed also in crystal structures of the ene reductase XenA with selected biomimetics. In selected cases, these biomimetics outperform the natural coenzymes. "Better-than-Nature" biomimetics should find widespread application in fine and specialty chemicals production by harnessing the power of high stereo-, regio-, and chemoselective redox biocatalysts and enabling reactions under mild conditions at low cost.

Nanoparticle-based biologic mimetics

PubMed Central

Cliffel, David E.; Turner, Brian N.; Huffman, Brian J.

2009-01-01

Centered on solid chemistry foundations, biology and materials science have reached a crossroad where bottom-up designs of new biologically important nanomaterials are a reality. The topics discussed here present the interdisciplinary field of creating biological mimics. Specifically, this discussion focuses on mimics that are developed using various types of metal nanoparticles (particularly gold) through facile synthetic methods. These methods conjugate biologically relevant molecules, e.g., small molecules, peptides, proteins, and carbohydrates, in conformationally favorable orientations on the particle surface. These new products provide stable, safe, and effective substitutes for working with potentially hazardous biologicals for applications such as drug targeting, immunological studies, biosensor development, and biocatalysis. Many standard bioanalytical techniques can be used to characterize and validate the efficacy of these new materials, including quartz crystal microbalance (QCM), surface plasmon resonance (SPR), and enzyme-linked immunosorbent assay (ELISA). Metal nanoparticle–based biomimetics continue to be developed as potential replacements for the native biomolecule in applications of immunoassays and catalysis. PMID:20049778

Protease activation in glycerol-based deep eutectic solvents.

PubMed

Zhao, Hua; Baker, Gary A; Holmes, Shaletha

2011-11-01

Deep eutectic solvents (DESs) consisting of mixtures of a choline salt (chloride or acetate form) and glycerol are prepared as easily accessible, biodegradable, and inexpensive alternatives to conventional aprotic cation-anion paired ionic liquids. These DES systems display excellent fluidity coupled with thermal stability to nearly 200 °C. In this work, the transesterification activities of cross-linked proteases (subtilisin and α-chymotrypsin), immobilized on chitosan, were individually examined in these novel DESs. In the 1:2 molar ratio mixture of choline chloride/glycerol containing 3% (v/v) water, cross-linked subtilisin exhibited an excellent activity (2.9 μmo l min(-1) g(-1)) in conjunction with a selectivity of 98% in the transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol. These highly encouraging results advocate more extensive exploration of DESs in protease-mediated biotransformations of additional polar substrates and use of DESs in biocatalysis more generally.

Bioinspired construction of multi-enzyme catalytic systems.

PubMed

Shi, Jiafu; Wu, Yizhou; Zhang, Shaohua; Tian, Yu; Yang, Dong; Jiang, Zhongyi

2018-06-18

Enzyme catalysis, as a green, efficient process, displays exceptional functionality, adaptivity and sustainability. Multi-enzyme catalysis, which can accomplish the tandem synthesis of valuable materials/chemicals from renewable feedstocks, establishes a bridge between single-enzyme catalysis and whole-cell catalysis. Multi-enzyme catalysis occupies a unique and indispensable position in the realm of biological reactions for energy and environmental applications. Two complementary strategies, i.e., compartmentalization and substrate channeling, have been evolved by living organisms for implementing the complex in vivo multi-enzyme reactions (MERs), which have been applied to construct multi-enzyme catalytic systems (MECSs) with superior catalytic activity and stabilities in practical biocatalysis. This tutorial review aims to present the recent advances and future prospects in this burgeoning research area, stressing the features and applications of the two strategies for constructing MECSs and implementing in vitro MERs. The concluding remarks are presented with a perspective on the construction of MECSs through rational combination of compartmentalization and substrate channeling.

Challenging Density Functional Theory Calculations with Hemes and Porphyrins.

PubMed

de Visser, Sam P; Stillman, Martin J

2016-04-07

In this paper we review recent advances in computational chemistry and specifically focus on the chemical description of heme proteins and synthetic porphyrins that act as both mimics of natural processes and technological uses. These are challenging biochemical systems involved in electron transfer as well as biocatalysis processes. In recent years computational tools have improved considerably and now can reproduce experimental spectroscopic and reactivity studies within a reasonable error margin (several kcal·mol(-1)). This paper gives recent examples from our groups, where we investigated heme and synthetic metal-porphyrin systems. The four case studies highlight how computational modelling can correctly reproduce experimental product distributions, predicted reactivity trends and guide interpretation of electronic structures of complex systems. The case studies focus on the calculations of a variety of spectroscopic features of porphyrins and show how computational modelling gives important insight that explains the experimental spectra and can lead to the design of porphyrins with tuned properties.

Oxide nanomaterials: synthetic developments, mechanistic studies, and technological innovations.

PubMed

Patzke, Greta R; Zhou, Ying; Kontic, Roman; Conrad, Franziska

2011-01-24

Oxide nanomaterials are indispensable for nanotechnological innovations, because they combine an infinite variety of structural motifs and properties with manifold morphological features. Given that new oxide materials are almost reported on a daily basis, considerable synthetic and technological work remains to be done to fully exploit this ever increasing family of compounds for innovative nano-applications. This calls for reliable and scalable preparative approaches to oxide nanomaterials and their development remains a challenge for many complex nanostructured oxides. Oxide nanomaterials with special physicochemical features and unusual morphologies are still difficult to access by classic synthetic pathways. The limitless options for creating nano-oxide building blocks open up new technological perspectives with the potential to revolutionize areas ranging from data processing to biocatalysis. Oxide nanotechnology of the 21st century thus needs a strong interplay of preparative creativity, analytical skills, and new ideas for synergistic implementations. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Effects of PEGylation on biomimetic synthesis of magnetoferritin nanoparticles

NASA Astrophysics Data System (ADS)

Yang, Caiyun; Cao, Changqian; Cai, Yao; Xu, Huangtao; Zhang, Tongwei; Pan, Yongxin

2017-03-01

Recent studies have demonstrated that ferrimagnetic magnetoferritin nanoparticles are a promising novel magnetic nanomaterial in biomedical applications, including biocatalysis, imaging, diagnostics, and tumor therapy. Here we investigated the PEGylation of human H-ferritin (HFn) proteins and the possible influence on biomimetic synthesis of magnetoferritin nanoparticles. The outer surface of HFn proteins was chemically modified with different PEG molecular weights (PEG10K and PEG20K) and different modification ratios (HFn subunit:PEG20K = 1:1, 1:2, 1:4). The PEGylated HFn proteins were used for biomimetic synthesis of ferrimagnetic magnetoferritin nanoparticles. We found that, compared with magnetoferritin using non-PEGylated HFn protein templates, the synthesized magnetoferritin using the PEGylated HFn protein templates possessed larger magnetite cores, higher magnetization and relaxivity values, and improved thermal stability. These results suggest that the PEGylation of H-ferritin may improve the biomineralization of magnetoferritin nanoparticles and enhance their biomedical applications.

Functionalized nanocompartments (Synthosomes): limitations and prospective applications in industrial biotechnology.

PubMed

Onaca, Ozana; Nallani, Madhavan; Ihle, Saskia; Schenk, Alexander; Schwaneberg, Ulrich

2006-01-01

Synthosomes are mechanically stable vesicles with a block copolymer membrane and an engineered transmembrane protein acting as selective gate. The polymer vesicles are nanometer-sized (50-1000 nm) and functionalized by loading them with enzymes for bioconversions or encapsulating charged macromolecules for selective compound recovery/release. The Synthosome system might become a novel technology platform for biocatalysis and selective product recovery. Progress in Synthosome research comprises employed block copolymers, transmembrane channel engineering, and functionalizations, which are discussed here in detail. The challenges in transmembrane protein engineering, as well as cost-effective production, in block copolymer design and the state of the art in Synthosome characterization comprising quantification of encapsulated protein, translocation efficiency, number of transmembrane channels per vesicle, and enzyme kinetics are also presented and discussed. An assessment of the Synthosome technology platform for prospective applications in industrial (white) biotechnology concludes this review.

Biosensors with Built-In Biomolecular Logic Gates for Practical Applications

PubMed Central

Lai, Yu-Hsuan; Sun, Sin-Cih; Chuang, Min-Chieh

2014-01-01

Molecular logic gates, designs constructed with biological and chemical molecules, have emerged as an alternative computing approach to silicon-based logic operations. These molecular computers are capable of receiving and integrating multiple stimuli of biochemical significance to generate a definitive output, opening a new research avenue to advanced diagnostics and therapeutics which demand handling of complex factors and precise control. In molecularly gated devices, Boolean logic computations can be activated by specific inputs and accurately processed via bio-recognition, bio-catalysis, and selective chemical reactions. In this review, we survey recent advances of the molecular logic approaches to practical applications of biosensors, including designs constructed with proteins, enzymes, nucleic acids, nanomaterials, and organic compounds, as well as the research avenues for future development of digitally operating “sense and act” schemes that logically process biochemical signals through networked circuits to implement intelligent control systems. PMID:25587423

Preparation of hollow silica nanospheres in O/W microemulsion system by hydrothermal temperature changes

NASA Astrophysics Data System (ADS)

Wang, Dandan; Li, Xiuyan; Liu, Zuohua; Shi, Xue; Zhou, Guowei

2017-01-01

Hollow silica nanospheres with wrinkled or smooth surfaces were successfully fabricated through a hydrothermal method. In this method, oil-in-water microemulsion (composed of cyclohexane, water, ethanol, and cetyltrimethylammonium bromide), and polyvinylpyrrolidone were utilized as template and capping agent, respectively. In such a facile synthesis, we can well realize the morphological transformation of spheres with radially oriented mesochannels to hollow structures of silica nanoparticle only by regulating the hydrothermal temperature from 100 °C to 200 °C. Synthesized samples with different mesostructures were then used as supports to immobilize Candida rugosa lipase (CRL). The immobilized CRL was employed as a new biocatalyst for biodiesel production through the esterification of heptanoic acid with ethanol. The conversion ratio of heptanoic acid with ethanol catalyzed by the immobilized CRL was also evaluated. Results of this study suggest that the prepared samples have potential applications in biocatalysis.

Recent Advances in Lipase-Mediated Preparation of Pharmaceuticals and Their Intermediates

PubMed Central

Carvalho, Ana Caroline Lustosa de Melo; Fonseca, Thiago de Sousa; de Mattos, Marcos Carlos; de Oliveira, Maria da Conceição Ferreira; de Lemos, Telma Leda Gomes; Molinari, Francesco; Romano, Diego; Serra, Immacolata

2015-01-01

Biocatalysis offers an alternative approach to conventional chemical processes for the production of single-isomer chiral drugs. Lipases are one of the most used enzymes in the synthesis of enantiomerically pure intermediates. The use of this type of enzyme is mainly due to the characteristics of their regio-, chemo- and enantioselectivity in the resolution process of racemates, without the use of cofactors. Moreover, this class of enzymes has generally excellent stability in the presence of organic solvents, facilitating the solubility of the organic substrate to be modified. Further improvements and new applications have been achieved in the syntheses of biologically active compounds catalyzed by lipases. This review critically reports and discusses examples from recent literature (2007 to mid-2015), concerning the synthesis of enantiomerically pure active pharmaceutical ingredients (APIs) and their intermediates in which the key step involves the action of a lipase. PMID:26690428

Rational assignment of key motifs for function guides in silico enzyme identification.

PubMed

Höhne, Matthias; Schätzle, Sebastian; Jochens, Helge; Robins, Karen; Bornscheuer, Uwe T

2010-11-01

Biocatalysis has emerged as a powerful alternative to traditional chemistry, especially for asymmetric synthesis. One key requirement during process development is the discovery of a biocatalyst with an appropriate enantiopreference and enantioselectivity, which can be achieved, for instance, by protein engineering or screening of metagenome libraries. We have developed an in silico strategy for a sequence-based prediction of substrate specificity and enantiopreference. First, we used rational protein design to predict key amino acid substitutions that indicate the desired activity. Then, we searched protein databases for proteins already carrying these mutations instead of constructing the corresponding mutants in the laboratory. This methodology exploits the fact that naturally evolved proteins have undergone selection over millions of years, which has resulted in highly optimized catalysts. Using this in silico approach, we have discovered 17 (R)-selective amine transaminases, which catalyzed the synthesis of several (R)-amines with excellent optical purity up to >99% enantiomeric excess.

NREL Advancements in Methane Conversion Lead to Cleaner Air, Useful Products

DOE Office of Scientific and Technical Information (OSTI.GOV)

2016-06-01

Researchers at NREL leveraged the recent on-site development of gas fermentation capabilities and novel genetic tools to directly convert methane to lactic acid using an engineered methanotrophic bacterium. The results provide proof-of-concept data for a gas-to-liquids bioprocess that concurrently produces fuels and chemicals from methane. NREL researchers developed genetic tools to express heterologous genes in methanotrophic organisms, which have historically been difficult to genetically engineer. Using these tools, researchers demonstrated microbial conversion of methane to lactate, a high-volume biochemical precursor predominantly utilized for the production of bioplastics. Methane biocatalysis offers a means to concurrently liquefy and upgrade natural gas andmore » renewable biogas, enabling their utilization in conventional transportation and industrial manufacturing infrastructure. Producing chemicals and fuels from methane expands the suite of products currently generated from biorefineries, municipalities, and agricultural operations, with the potential to increase revenue and significantly reduce greenhouse gas emissions.« less

Retuning the Catalytic Bias and Overpotential of a [NiFe]-Hydrogenase via a Single Amino Acid Exchange at the Electron Entry/Exit Site

PubMed Central

2017-01-01

The redox chemistry of the electron entry/exit site in Escherichia coli hydrogenase-1 is shown to play a vital role in tuning biocatalysis. Inspired by nature, we generate a HyaA-R193L variant to disrupt a proposed Arg–His cation−π interaction in the secondary coordination sphere of the outermost, “distal”, iron–sulfur cluster. This rewires the enzyme, enhancing the relative rate of H2 production and the thermodynamic efficiency of H2 oxidation catalysis. On the basis of Fourier transformed alternating current voltammetry measurements, we relate these changes in catalysis to a shift in the distal [Fe4S4]2+/1+ redox potential, a previously experimentally inaccessible parameter. Thus, metalloenzyme chemistry is shown to be tuned by the second coordination sphere of an electron transfer site distant from the catalytic center. PMID:28697596

Ultrasonic treatment of Viscozyme Cassava C preparation for improving cellulase activity

NASA Astrophysics Data System (ADS)

Tra, Tran Thi Thu; Vu, Huynh Minh; Man, Le Van Viet

2017-09-01

In this study, the effects of ultrasonic treatment on the cellulolytic activity of Viscozyme Cassava C preparation were investigated. The biocatalyst was treated with ultrasound at different enzyme concentrations (from 0.02 to 19.50 mg protein/mL), ultrasonic powers (from 0 to 12 W/mL) and times (from 0 to 120 seconds). The highest cellulase activity was achieved when the enzyme preparation was ultrasonicated at 7.3 W/mL for 40 sec, under which the cellulase activity increased by 18.1% over the control. The optimal pH and temperature of the sonicated and unsonicated biocatalysts were statistically similar. However, the half-life value of the sonicated preparation at 4 °C was 24.5% higher than that of the unsonicated preparation. This result indicated that ultrasonic treatment of the enzyme preparation could reduce its amount used in biocatalysis.

Biocatalytic induction of supramolecular order

NASA Astrophysics Data System (ADS)

Hirst, Andrew R.; Roy, Sangita; Arora, Meenakshi; Das, Apurba K.; Hodson, Nigel; Murray, Paul; Marshall, Stephen; Javid, Nadeem; Sefcik, Jan; Boekhoven, Job; van Esch, Jan H.; Santabarbara, Stefano; Hunt, Neil T.; Ulijn, Rein V.

2010-12-01

Supramolecular gels, which demonstrate tunable functionalities, have attracted much interest in a range of areas, including healthcare, environmental protection and energy-related technologies. Preparing these materials in a reliable manner is challenging, with an increased level of kinetic defects observed at higher self-assembly rates. Here, by combining biocatalysis and molecular self-assembly, we have shown the ability to more quickly access higher-ordered structures. By simply increasing enzyme concentration, supramolecular order expressed at molecular, nano- and micro-levels is dramatically enhanced, and, importantly, the gelator concentrations remain identical. Amphiphile molecules were prepared by attaching an aromatic moiety to a dipeptide backbone capped with a methyl ester. Their self-assembly was induced by an enzyme that hydrolysed the ester. Different enzyme concentrations altered the catalytic activity and size of the enzyme clusters, affecting their mobility. This allowed structurally diverse materials that represent local minima in the free energy landscape to be accessed based on a single gelator structure.

New Sustainable Model of Biorefineries: Biofactories and Challenges of Integrating Bio- and Solar Refineries.

PubMed

Abate, Salvatore; Lanzafame, Paola; Perathoner, Siglinda; Centi, Gabriele

2015-09-07

The new scenario for sustainable (low-carbon) chemical and energy production drives the development of new biorefinery concepts (indicated as biofactories) with chemical production at the core, but flexible and small-scale production. An important element is also the integration of solar energy and CO2 use within biobased production. This concept paper, after shortly introducing the motivation and recent trends in this area, particularly at the industrial scale, and some of the possible models (olefin and intermediate/high-added-value chemicals production), discusses the opportunities and needs for research to address the challenge of integrating bio- and solar refineries. Aspects discussed regard the use of microalgae and CO2 valorization in biorefineries/biofactories by chemo- or biocatalysis, including possibilities for their synergetic cooperation and symbiosis, as well as integration within the agroenergy value chain. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions.

PubMed

Liu, Jiaheng; Li, Huiling; Zhao, Guangrong; Caiyin, Qinggele; Qiao, Jianjun

2018-05-01

NAD and NADP, a pivotal class of cofactors, which function as essential electron donors or acceptors in all biological organisms, drive considerable catabolic and anabolic reactions. Furthermore, they play critical roles in maintaining intracellular redox homeostasis. However, many metabolic engineering efforts in industrial microorganisms towards modification or introduction of metabolic pathways, especially those involving consumption, generation or transformation of NAD/NADP, often induce fluctuations in redox state, which dramatically impede cellular metabolism, resulting in decreased growth performance and biosynthetic capacity. Here, we comprehensively review the cofactor engineering strategies for solving the problematic redox imbalance in metabolism modification, as well as their features, suitabilities and recent applications. Some representative examples of in vitro biocatalysis are also described. In addition, we briefly discuss how tools and methods from the field of synthetic biology can be applied for cofactor engineering. Finally, future directions and challenges for development of cofactor redox engineering are presented.

Biotransformation of (-)-(1R,4S)-Menthone and (+)-(1S,4R)-Menthone by the Common Cutworm Spodoptera litura Larvae.

PubMed

Marumoto, Shinsuke; Okuno, Yoshiharu; Hagiwara, Yuki; Miyazawa, Mitsuo

2017-08-01

Using biotransformation as a biocatalytic process has the advantage of being able to proceed under mild conditions and with high regio- and enantioselectivity. This study investigated the biotransformation of (-)-(1R,4S)-menthone (1) and (+)-(1S,4R)-menthone (2) by Spodoptera litura larvae. Compound 1 was converted to (-)-(1R,4S)-7-hydroxymenthone (1-1), (+)-(1R,3S,4S)-7-hydroxyneomenthol (1-2) and (-)-(1R,4S,8R)-p-menth-3-one-9-oic acid (1-3). The metabolism of substrate 2 generated three enantiomers of the above metabolites, designated as 2-1 to 2-3, respectively. The C-9 position of (-)-menthone and (+)-menthone was oxidized to carboxylic acid by S. litura, which is a metabolic pathway not observed in any other example of biocatalysis.

Catalytic mechanism of phenylacetone monooxygenases for non-native linear substrates.

PubMed

Carvalho, Alexandra T P; Dourado, Daniel F A R; Skvortsov, Timofey; de Abreu, Miguel; Ferguson, Lyndsey J; Quinn, Derek J; Moody, Thomas S; Huang, Meilan

2017-10-11

Phenylacetone monooxygenase (PAMO) is the most stable and thermo-tolerant member of the Baeyer-Villiger monooxygenase family, and therefore it is an ideal candidate for the synthesis of industrially relevant compounds. However, its limited substrate scope has largely limited its industrial applications. In the present work, we provide, for the first time, the catalytic mechanism of PAMO for the native substrate phenylacetone as well as for a linear non-native substrate 2-octanone, using molecular dynamics simulations, quantum mechanics and quantum mechanics/molecular mechanics calculations. We provide a theoretical basis for the preference of the enzyme for the native aromatic substrate over non-native linear substrates. Our study provides fundamental atomic-level insights that can be employed in the rational engineering of PAMO for wide applications in industrial biocatalysis, in particular, in the biotransformation of long-chain aliphatic oils into potential biodiesels.

Computational predictions of the new Gallium nitride nanoporous structures

NASA Astrophysics Data System (ADS)

Lien, Le Thi Hong; Tuoc, Vu Ngoc; Duong, Do Thi; Thu Huyen, Nguyen

2018-05-01

Nanoporous structural prediction is emerging area of research because of their advantages for a wide range of materials science and technology applications in opto-electronics, environment, sensors, shape-selective and bio-catalysis, to name just a few. We propose a computationally and technically feasible approach for predicting Gallium nitride nanoporous structures with hollows at the nano scale. The designed porous structures are studied with computations using the density functional tight binding (DFTB) and conventional density functional theory methods, revealing a variety of promising mechanical and electronic properties, which can potentially find future realistic applications. Their stability is discussed by means of the free energy computed within the lattice-dynamics approach. Our calculations also indicate that all the reported hollow structures are wide band gap semiconductors in the same fashion with their parent’s bulk stable phase. The electronic band structures of these nanoporous structures are finally examined in detail.

A green approach to the synthesis of novel ``Desert rose stone''-like nanobiocatalytic system with excellent enzyme activity and stability

NASA Astrophysics Data System (ADS)

Wang, Min; Bao, Wen-Jing; Wang, Jiong; Wang, Kang; Xu, Jing-Juan; Chen, Hong-Yuan; Xia, Xing-Hua

2014-10-01

3D hierarchical layer double hydroxides (LDHs) have attracted extensive interest due to their unique electronic and catalytic properties. Unfortunately, the existing preparation methods require high temperature or toxic organic compounds, which limits the applications of the 3D hierarchical LDHs in biocatalysis and biomedicine. Herein, we present a green strategy to synthesize ``Desert Rose Stone''-like Mg-Al-CO3 LDH nanoflowers in situ deposited on aluminum substrates via a coprecipitation method using atmospheric carbon dioxide. Using this method, we construct a novel ``Desert Rose Stone''-like nanobiocatalytic system by using HRP as the model enzyme. Compared with the free HRP, the HRP/Mg-Al-LDH nanobiocatalytic system exhibits higher catalytic activity and stability. A smaller apparent Michaelis-Menten constant (0.16 mM) of this system suggests that the encapsulated HRP shows higher affinity towards H2O2.

Biocatalysis of aromatic benzyl-propionate ester by different immobilized lipases.

PubMed

Sá, Amanda Gomes Almeida; de Meneses, Alessandra Cristina; Lerin, Lindomar Alberto; de Araújo, Pedro Henrique Hermes; Sayer, Cláudia; de Oliveira, Débora

2018-05-01

Benzyl propionate is an aromatic ester that possesses a fruity odor and is usually found in nature in the composition of some fruits such as plums and melons. This work aimed for the benzyl propionate synthesis by esterification using a new immobilized enzyme preparation with low-cost material from Candida antarctica (NS 88011) and three commercial immobilized lipases (Novozym 435, Lipozyme TL-IM and Lipozyme RM-IM). Novozym 435 had the best performance even when the solvent tert-butanol was absent of the reaction medium. Results from a 2 2 factorial design showed that an increase in the enzyme amount led to a higher conversion, even when the temperature was kept at the low value. Currently, no research had synthesized successfully benzyl propionate via esterification mediated by lipases; and we reached an ester conversion of ~ 44% after 24 h indicating that it is a promising route for benzyl propionate biotechnological production.

Sequence-controlled methacrylic multiblock copolymers via sulfur-free RAFT emulsion polymerization

NASA Astrophysics Data System (ADS)

Engelis, Nikolaos G.; Anastasaki, Athina; Nurumbetov, Gabit; Truong, Nghia P.; Nikolaou, Vasiliki; Shegiwal, Ataulla; Whittaker, Michael R.; Davis, Thomas P.; Haddleton, David M.

2017-02-01

Translating the precise monomer sequence control achieved in nature over macromolecular structure (for example, DNA) to whole synthetic systems has been limited due to the lack of efficient synthetic methodologies. So far, chemists have only been able to synthesize monomer sequence-controlled macromolecules by means of complex, time-consuming and iterative chemical strategies such as solid-state Merrifield-type approaches or molecularly dissolved solution-phase systems. Here, we report a rapid and quantitative synthesis of sequence-controlled multiblock polymers in discrete stable nanoscale compartments via an emulsion polymerization approach in which a vinyl-terminated macromolecule is used as an efficient chain-transfer agent. This approach is environmentally friendly, fully translatable to industry and thus represents a significant advance in the development of complex macromolecule synthesis, where a high level of molecular precision or monomer sequence control confers potential for molecular targeting, recognition and biocatalysis, as well as molecular information storage.

The DNP project: Quandaries for nursing scholars.

PubMed

Dols, Jean Dowling; Hernández, Christina; Miles, Heather

In the evolving Doctor of Nursing Practice (DNP) movement, there continues to be a lack of agreement about the final scholarly project. This study identifies and describes the faculty practices and challenges related to the DNP project across the United States. In a descriptive research study, 90 DNP program directors responded to an online survey describing the environment of the DNP program with emphasis on the final scholarly project. According to the respondents, 87% of faculty are somewhat or very dissatisfied with the DNP project. Elements that may contribute to the dissatisfaction are the reported lack of faculty knowledge of evidence-based practice and quality improvement, lack of consensus on the DNP project, lack of faculty resources for DNP projects, challenges with clinical sites for the DNP project, and students' scholarly writing skills. It is imperative to have academic/practice faculty oriented to DNP concepts; achieve consensus on the project title, type, depth, and outcomes; and have an ongoing dialog regarding DNP project design, execution, and challenges. Project implementation models need to be appropriate for the escalating DNP enrollment. Program support related to institutional review board relationships, student writing and statistical skills, and program-practice site partnerships are needed. Copyright © 2016 Elsevier Inc. All rights reserved.

NASA high performance computing and communications program

NASA Technical Reports Server (NTRS)

Holcomb, Lee; Smith, Paul; Hunter, Paul

1993-01-01

The National Aeronautics and Space Administration's HPCC program is part of a new Presidential initiative aimed at producing a 1000-fold increase in supercomputing speed and a 100-fold improvement in available communications capability by 1997. As more advanced technologies are developed under the HPCC program, they will be used to solve NASA's 'Grand Challenge' problems, which include improving the design and simulation of advanced aerospace vehicles, allowing people at remote locations to communicate more effectively and share information, increasing scientist's abilities to model the Earth's climate and forecast global environmental trends, and improving the development of advanced spacecraft. NASA's HPCC program is organized into three projects which are unique to the agency's mission: the Computational Aerosciences (CAS) project, the Earth and Space Sciences (ESS) project, and the Remote Exploration and Experimentation (REE) project. An additional project, the Basic Research and Human Resources (BRHR) project exists to promote long term research in computer science and engineering and to increase the pool of trained personnel in a variety of scientific disciplines. This document presents an overview of the objectives and organization of these projects as well as summaries of individual research and development programs within each project.

Manufacturing Methods and Technology (MMT) project execution report

NASA Astrophysics Data System (ADS)

Swim, P. A.

1982-10-01

This document is a summary compilation of the manufacturing methods and technology program project status reports (RCS DRCMT-301) submitted to IBEA from DARCOM major Army subcommands and project managers. Each page of the computerized section lists project number, title, status, funding, and projected completion date. Summary pages give information relating to the overall DARCOM program.

34 CFR 664.13 - What is a group research or study project?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 34 Education 3 2012-07-01 2012-07-01 false What is a group research or study project? 664.13... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM What Kinds of Projects Does the Secretary Assist Under This Program? § 664.13 What is a group research or study project...

34 CFR 664.13 - What is a group research or study project?

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 3 2014-07-01 2014-07-01 false What is a group research or study project? 664.13... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM What Kinds of Projects Does the Secretary Assist Under This Program? § 664.13 What is a group research or study project...

34 CFR 664.13 - What is a group research or study project?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 34 Education 3 2013-07-01 2013-07-01 false What is a group research or study project? 664.13... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM What Kinds of Projects Does the Secretary Assist Under This Program? § 664.13 What is a group research or study project...

34 CFR 664.13 - What is a group research or study project?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false What is a group research or study project? 664.13... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM What Kinds of Projects Does the Secretary Assist Under This Program? § 664.13 What is a group research or study project...

34 CFR 664.13 - What is a group research or study project?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false What is a group research or study project? 664.13... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD PROGRAM What Kinds of Projects Does the Secretary Assist Under This Program? § 664.13 What is a group research or study project...

24 CFR 266.520 - Program monitoring and compliance.

Code of Federal Regulations, 2010 CFR

2010-04-01

... AUTHORITIES HOUSING FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Project Management and Servicing § 266.520 Program monitoring and compliance. HUD will monitor the...

14 CFR 151.71 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-01-01

... FEDERAL AID TO AIRPORTS Project Programming Standards § 151.71 Applicability. (a) This subpart prescribes programming and design and construction standards for projects under the Federal-Aid Airport Program to assure...

14 CFR 151.71 - Applicability.

Code of Federal Regulations, 2012 CFR

2012-01-01

... FEDERAL AID TO AIRPORTS Project Programming Standards § 151.71 Applicability. (a) This subpart prescribes programming and design and construction standards for projects under the Federal-Aid Airport Program to assure...

14 CFR 151.71 - Applicability.

Code of Federal Regulations, 2013 CFR

2013-01-01

... FEDERAL AID TO AIRPORTS Project Programming Standards § 151.71 Applicability. (a) This subpart prescribes programming and design and construction standards for projects under the Federal-Aid Airport Program to assure...

24 CFR 266.520 - Program monitoring and compliance.

Code of Federal Regulations, 2012 CFR

2012-04-01

... AUTHORITIES HOUSING FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Project Management and Servicing § 266.520 Program monitoring and compliance. HUD will monitor the...

24 CFR 266.520 - Program monitoring and compliance.

Code of Federal Regulations, 2011 CFR

2011-04-01

... AUTHORITIES HOUSING FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Project Management and Servicing § 266.520 Program monitoring and compliance. HUD will monitor the...

24 CFR 266.520 - Program monitoring and compliance.

Code of Federal Regulations, 2013 CFR

2013-04-01

... AUTHORITIES HOUSING FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Project Management and Servicing § 266.520 Program monitoring and compliance. HUD will monitor the...

24 CFR 266.520 - Program monitoring and compliance.

Code of Federal Regulations, 2014 CFR

2014-04-01

... AUTHORITIES HOUSING FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Project Management and Servicing § 266.520 Program monitoring and compliance. HUD will monitor the...

14 CFR 151.71 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-01-01

... FEDERAL AID TO AIRPORTS Project Programming Standards § 151.71 Applicability. (a) This subpart prescribes programming and design and construction standards for projects under the Federal-Aid Airport Program to assure...

14 CFR 151.71 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-01-01

... FEDERAL AID TO AIRPORTS Project Programming Standards § 151.71 Applicability. (a) This subpart prescribes programming and design and construction standards for projects under the Federal-Aid Airport Program to assure...

Columbia River Basin Fish and Wildlife Program Annual Implementation Work Plan for Fiscal Year 1990.

DOE Office of Scientific and Technical Information (OSTI.GOV)

United States. Bonneville Power Administration; Northwest Power Planning Council; Columbia Basin Fish and Wildlife Authority

1990-01-01

The Columbia River Basin Fish and Wildlife Program (Program) was developed by the Northwest Power Planning Council (Council) in accordance with Public Law 96-501, the Pacific Northwest Electric Power Planning and Conservation Act (Act). The purpose of the Program is to guide the Bonneville Power Administration (BPA) and other Federal agencies in carrying out their responsibilities to protect, mitigate, and enhance fish and wildlife of the Columbia River Basin. The Act explicitly gives BPA the authority and responsibility to use the BPA fund for these ends, to the extent that fish and wildlife are affected by the development and operationmore » of hydroelectric generation in the Columbia River Basin. This document presents BPA's plans for implementing the Program during Fiscal Year (FY) 1990. The Columbia River Basin Fish and Wildlife Program Annual Implementation Work Plan (AIWP) reflects the primary goals of the Council's Action Plan (Section 1400 of the Program): to provide a solid, timely, and focused basis for budgeting and planning. In addition, the AIWP provides a means to judge progress and the success of Program implementation. The FY 1990 AIWP also follows the outline developed by the Policy Review Group (PRG) during Step 1 of initial cycle of the Implementation Planning Process (IPP), which is described in Section III. A number of new FY 1990 projects were still under review by the PRG as the AIWP went to press. These projects have been noted in Table 2, New FY 1990 Program Projects, and in the text of the AIWP. This AIWP has been organized and written to meet the specific needs of Program Action Items 10.1-10.3. The AIWP includes schedules with key milestones for FY 1990 and beyond, and addresses the Action Items assigned to BPA in Section 1400 of the 1987 Program. All BPA-funded Program projects discussed in the FY 1990 AIWP are listed in Tables 1 and 2 according to their status as of September 30, 1989. Table 1 (pp. 3-14) lists completed, ongoing, and deferred projects. Table 2 (pp. 15-18) lists all projects which BPA plans to fund as ''new'' projects in FY 1990. ''Ongoing'' status indicates that the project started in FY 1989 or before, and that it is expected to continue in FY 1990. ''Deferred'' means that BPA implementation has been postponed to FY 1991 or later. ''Completed'' indicates completion during FY 1989. ''New'' denotes projects that BPA plans to start in FY 1990. One new FY 1990 project was included in the draft FY 1990 AIWP as a ''conditional'' project, as a result of PRG recommendations at the May 25, 1989, PRG meeting. If specified conditions are met, BPA will begin to implement the project in FY 1990. Section VII describes BPA's non-Program, internal support projects. These projects were not subject to review by the PRG and have been included in the AIWP to help the PRG and the public to better understand what BPA is doing.« less

76 FR 66284 - Wind and Water Power Program

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-26

... projects and the overall Water Power Program research portfolio, a report will be compiled by DOE, which... DEPARTMENT OF ENERGY Office of Energy Efficiency and Renewable Energy Wind and Water Power Program... projects. The 2011 Wind and Water Power Program, Water Power Peer Review Meeting will review the Program's...

ASK Magazine. Volume 10

NASA Technical Reports Server (NTRS)

Hoffman, Edward (Editor); Laufer, Alexander (Editor); Post, Todd (Editor); Brady, Jody Lannen (Editor)

2003-01-01

The Academy of Program and Project Leadership (APPL) and ASK Magazine is presented. APPL is a research-based organization that serves NASA program and project managers, as well as project teams, at every level of development. In 1997, APPL was created from an earlier program to underscore the importance that NASA places on project management and project teams through a wide variety of products and services, including knowledge sharing, classroom and online courses, career development guidance, performance support, university partnerships, and advanced technology tools. ASK Magazine grew out of our Knowledge Sharing Initiative. The stories that appear in ASK are written by the 'best of the best' project managers, primarily from NASA, but also from other government agencies and industry. These stories contain genuine nuggets of knowledge and wisdom that are transferable across projects. Who better than a project manager to help another project manager address a critical issue on a project? Big projects, small projects-they're all here in ASK. APPL is one of our most exciting publications about project management.

Evaluation of the Florida coordinated school health program pilot schools project.

PubMed

Weiler, Robert M; Pigg, R Morgan; McDermott, Robert J

2003-01-01

The Florida Department of Education, with CDC funding, designed the Florida Coordinated School Health Program Pilot Schools Project (PSP) to encourage innovative approaches to promote coordinated school health programs (CSHP) in Florida schools. Each of eight pilot schools received $15,000 in project funding, three years of technical assistance including on-site and off-site assistance, a project office resource center, mailings of resource materials, needs assessment and evaluation assistance, and three PSP Summer Institutes. Project evaluators created a context evaluation, approaching each school independently as a "case study" to measure the school's progress in meeting goals established at baseline. Data were collected using the How Healthy is Your School? needs assessment instrument, a School Health Portfolio constructed by each school team, a Pilot Schools Project Team Member Survey instrument, midcourse team interviews, final team interviews, and performance indicator data obtained from pilot and control schools. The PSP posed two fundamental questions: "Can financial resources, professional training, and technical assistance enable individual schools to create and sustain a coordinated school health program?" and "What outcomes reasonably can one expect from a coordinated school health program, assuming programs receive adequate support over time?" First, activities at the eight schools confirmed that a coordinated school health programs can be established and sustained. Program strength and sustainability depend on long-term resources, qualified personnel, and administrative support. Second, though coordinated school health programs may improve school performance indicators, the PSP yielded insufficient evidence to support that belief. Future projects should include robust measurement and evaluation designs, thereby producing conclusive evidence about the influence of a coordinated school health program on such outcomes.

OBJECTIVES, DESIGN, AND PRELIMINARY RESULTS FROM THE ATLANTA SUPERSITE PROJECT

EPA Science Inventory

The Atlanta Supersites project is the first of two Supersites projects to be established during Phase I of EPA's Supersites Program; Phase II is being established through a Request for Assistance. The other initial project is in Fresno, California. The Supersite Program is part o...

12 CFR 952.5 - Community Investment Cash Advance Programs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... chapter. (2) Each Bank shall offer a CIP to provide financing for housing projects and for eligible... than CIP, involving a combination of housing projects and economic development projects, only the... respective CICA program. (2) For projects funded under CIP, both the housing and economic development...

12 CFR 952.5 - Community Investment Cash Advance Programs.

Code of Federal Regulations, 2012 CFR

2012-01-01

... chapter. (2) Each Bank shall offer a CIP to provide financing for housing projects and for eligible... than CIP, involving a combination of housing projects and economic development projects, only the... respective CICA program. (2) For projects funded under CIP, both the housing and economic development...

12 CFR 952.5 - Community Investment Cash Advance Programs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... chapter. (2) Each Bank shall offer a CIP to provide financing for housing projects and for eligible... than CIP, involving a combination of housing projects and economic development projects, only the... respective CICA program. (2) For projects funded under CIP, both the housing and economic development...

12 CFR 952.5 - Community Investment Cash Advance Programs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... chapter. (2) Each Bank shall offer a CIP to provide financing for housing projects and for eligible... than CIP, involving a combination of housing projects and economic development projects, only the... respective CICA program. (2) For projects funded under CIP, both the housing and economic development...

P.C.A.P. Project Profiles and General Profilles. Queensland Priority Country Area Program-Evaluation Series.

ERIC Educational Resources Information Center

Saide, Tom, Ed.

Thirty-eight projects designed to improve educational opportunities of rural Queensland children were initiated in 1977 and funded through the Disadvantaged Schools Program; the program was renamed the Country Area Program and made a permanent School Commission program in 1982. The program resulted from a 1977-79 Schools Commission report…

Project Parents: Awareness, Education, Involvement Program. O.E.E. Evaluation Report, 1981-1982.

ERIC Educational Resources Information Center

Lehman, Sheila; Frischholz, Edward J.

The Project Parents: Awareness, Education, Involvement Program is an educational program which is conducted by the New York City Schools and involves 144 parents with children in elementary level bilingual education programs or eligible for such programs. While the focus of the program is on parents, its goal is the enhanced educational…

Hypermedia and Research Methodology: An Associative Research Model. Final Report.

ERIC Educational Resources Information Center

City Univ. of New York, NY. Bernard Baruch Coll.

The purpose of the project described in this three-part report was to test the value of hypermedia for library instruction. The project included the development of a hypermedia program built around a marketing topic; implementation and testing of the program with one group of students assigned to use the new program; evaluation of the project by…

The Social Competency Program of the Reach Out to Schools Project. Project Report, 1991-92. No. 3.

ERIC Educational Resources Information Center

Krasnow, Jean H.; And Others

This document presents outcomes of the Social Competency Program: Reach Out to Schools Project, a program designed to help elementary students learn and practice interpersonal and problem-solving skills. It is based on the understanding that positive peer relationships and a supportive, caring classroom community are essential to students' social…

2017 TRIAD Small Business Advisory Panel

DTIC Science & Technology

2017-10-11

government service in 2007 as the Science & Technology (S&T) Project Manager for the United States Navy’s Unmanned Maritime Systems Program Office...National Renewable Energy Laboratory (NREL) as a Program Manager for over 110 energy and water projects on military installations. He worked... management , project management , program management , donor relation service, contract and subcontract policy development and implementation, data integrity

43 CFR 404.12 - Can Reclamation provide assistance with the construction of a rural water supply project under...

Code of Federal Regulations, 2010 CFR

2010-10-01

... the construction of a rural water supply project under this program? 404.12 Section 404.12 Public... RECLAMATION RURAL WATER SUPPLY PROGRAM Overview § 404.12 Can Reclamation provide assistance with the construction of a rural water supply project under this program? Reclamation may provide assistance with the...

Penn State's Comprehensive Bilingual Early Childhood Teacher Training Project. Results of Three Project Years, 1987-1990. Volume 1.

ERIC Educational Resources Information Center

Soto, Lourdes Diaz; Negron, Lillian

The results of a bilingual teacher training project developed to serve the needs of young limited-English-proficient children are presented. The report contains a program description, a rationale for program implementation, and program goals. Particular attention is focused on the following: demographic information, goal setting, course work,…

34 CFR 350.2 - What is the purpose of the Disability and Rehabilitation Research Project and Centers Program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What is the purpose of the Disability and..., DEPARTMENT OF EDUCATION DISABILITY AND REHABILITATION RESEARCH PROJECTS AND CENTERS PROGRAM General § 350.2 What is the purpose of the Disability and Rehabilitation Research Project and Centers Program? The...

Project COMPAS [Consortium for Operating and Managing Programs for the Advancment of Skills]: A Design for Change.

ERIC Educational Resources Information Center

Schermerhorn, Leora L., Ed.; And Others

Descriptive and evaluative information is provided in this report on Project COMPAS (Consortium for Operating and Managing Programs for the Advancement of Skills), a cooperative effort between seven community colleges which developed cognitive skills programs for entry-level freshmen. Chapter I reviews the unique features of Project COMPAS,…

Project Success Environment: A Practical Program for Implementing Behavior Modification in Urban Elementary Schools.

ERIC Educational Resources Information Center

Rollins, Howard; And Others

The results of a 3-year project that developed a practical program for the wide-scale implementation of behavior modification in urban schools are presented in this paper. The major outcomes of the project were (a) a practical, cost-effective behavior modification program that reduces discipline problems, increases student motivation, and…

The Power to Grow: Success Stories from the National Library Power Program.

ERIC Educational Resources Information Center

Sadowski, Michael

1994-01-01

Describes the National Library Program supported by the DeWitt Wallace-Reader's Digest Fund. The program's national goals and guidelines and results of eight projects are presented. Brief descriptions of five new projects and nine planning sites are given. Continuation plans and the possibility of funding beyond the three-year project period are…

New York State Energy Research and Development Authority. Research projects` update project status as of March 31, 1997

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1997-07-01

This report provides an update of the New York State Energy Research and Development Authority (NYSERDA) program. The NYSERDA research and development program has five major areas: industry, buildings, energy resources, transportation, and environment. NYSERDA organizes projects within these five major areas based on energy use and supply, and end-use sectors. Therefore, issues such as waste management, energy products and renewable energy technologies are addressed in several areas of the program. The project descriptions presented are organized within the five program areas. Descriptions of projects completed between the period April 1, 1996, and March 31, 1997, including technology-transfer activities, aremore » at the end of each subprogram section.« less

23 CFR 140.603 - Programs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Programs. 140.603 Section 140.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES REIMBURSEMENT Reimbursement for Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of...

23 CFR 140.603 - Programs.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 23 Highways 1 2013-04-01 2013-04-01 false Programs. 140.603 Section 140.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES REIMBURSEMENT Reimbursement for Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of...

23 CFR 140.603 - Programs.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 23 Highways 1 2014-04-01 2014-04-01 false Programs. 140.603 Section 140.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES REIMBURSEMENT Reimbursement for Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of...

RCP Local School Projects in South Carolina.

ERIC Educational Resources Information Center

Regional Curriculum Project, Atlanta, GA.

One of 6 state reports of projects and programs operating in cooperation with the Regional Curriculum Project, the document highlights major curriculum-change programs in South Carolina which were initiated in 1966. The 4 projects reported are "Curriculum Study in Berkeley County," which had as its purpose the identification and…

12 CFR 1292.5 - Community Investment Cash Advance Programs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... shall offer a CIP to provide financing for housing projects and for eligible targeted community lending... projects. (1) For projects funded under CICA programs other than CIP, involving a combination of housing... under CIP, both the housing and economic development components of the project must meet the appropriate...

50 CFR 84.30 - How are projects selected for grants?

Code of Federal Regulations, 2010 CFR

2010-10-01

... INTERIOR (CONTINUED) FINANCIAL ASSISTANCE-WILDLIFE SPORT FISH RESTORATION PROGRAM NATIONAL COASTAL WETLANDS CONSERVATION GRANT PROGRAM Project Selection § 84.30 How are projects selected for grants? Project selection is...) Provides public conservation benefits that are cost effective and long-term, i.e., at least 20 years; and...

7 CFR 3405.7 - Joint project proposals.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 15 2011-01-01 2011-01-01 false Joint project proposals. 3405.7 Section 3405.7... AGRICULTURE HIGHER EDUCATION CHALLENGE GRANTS PROGRAM Program Description § 3405.7 Joint project proposals. Applicants are encouraged to submit joint project proposals as defined in § 3405.2(m), which address regional...

China: Tradition and Transformation. Curriculum Projects. 1993 Fulbright Hays Seminar Abroad Program.

ERIC Educational Resources Information Center

National Committee on United States-China Relations, New York, NY.

Sixteen participants in the 1993 Fulbright Hays Seminar Abroad Program present curriculum projects that focus on tradition and transformation in China. The curriculum projects highlight the diversity of interests and disciplines of the participants and offer ideas for various educational levels. The projects include: (1) "Beijing…

7 CFR 1739.11 - Eligible project.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 11 2010-01-01 2010-01-01 false Eligible project. 1739.11 Section 1739.11 Agriculture... BROADBAND GRANT PROGRAM Community Connect Grant Program § 1739.11 Eligible project. To be eligible for a grant, the Project must: (a) Serve a Rural Area where Broadband Transmission Service does not currently...

Handicapped Children's Early Education Program. 1982-83 Overview and Directory.

ERIC Educational Resources Information Center

Assael, Daniel, Ed.

The document serves as a directory of federal projects in the Handicapped Children's Early Education Program (HCEEP). An introduction describes the HCEEP network and briefly summarizes results of a recent evaluation study. The overview section then describes the five types of HCEEP projects: deomonstration projects, outreach projects, state…

7 CFR 1739.11 - Eligible project.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 11 2011-01-01 2011-01-01 false Eligible project. 1739.11 Section 1739.11 Agriculture... BROADBAND GRANT PROGRAM Community Connect Grant Program § 1739.11 Eligible project. To be eligible for a grant, the Project must: (a) Serve a Rural Area where Broadband Transmission Service does not currently...

7 CFR 1739.11 - Eligible project.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 11 2012-01-01 2012-01-01 false Eligible project. 1739.11 Section 1739.11 Agriculture... BROADBAND GRANT PROGRAM Community Connect Grant Program § 1739.11 Eligible project. To be eligible for a grant, the Project must: (a) Serve a Rural Area where Broadband Transmission Service does not currently...

7 CFR 1739.11 - Eligible project.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 11 2013-01-01 2013-01-01 false Eligible project. 1739.11 Section 1739.11 Agriculture... BROADBAND GRANT PROGRAM Community Connect Grant Program § 1739.11 Eligible project. To be eligible for a grant, the Project must: (a) Serve a Rural Area where Broadband Transmission Service does not currently...

Project Senior. Innovations in Educational Programming for the Elderly. A Pilot Project: Thermopolis, Wyoming. [Final Report].

ERIC Educational Resources Information Center

Owsley, Jean

A pilot project, entitled Project Senior, was developed and implemented to provide innovative educational programing for older adults in the small rural setting of Thermopolis, Wyoming. Included among the major project activities were the following: a door-to-door survey of 759 persons over 55 years old to determine those courses most desired by…

Disease management projects and the Chronic Care Model in action: baseline qualitative research

PubMed Central

2012-01-01

Background Disease management programs, especially those based on the Chronic Care Model (CCM), are increasingly common in the Netherlands. While disease management programs have been well-researched quantitatively and economically, less qualitative research has been done. The overall aim of the study is to explore how disease management programs are implemented within primary care settings in the Netherlands; this paper focuses on the early development and implementation stages of five disease management programs in the primary care setting, based on interviews with project leadership teams. Methods Eleven semi-structured interviews were conducted at the five selected sites with sixteen professionals interviewed; all project directors and managers were interviewed. The interviews focused on each project’s chosen chronic illness (diabetes, eating disorders, COPD, multi-morbidity, CVRM) and project plan, barriers to development and implementation, the project leaders’ action and reactions, as well as their roles and responsibilities, and disease management strategies. Analysis was inductive and interpretive, based on the content of the interviews. After analysis, the results of this research on disease management programs and the Chronic Care Model are viewed from a traveling technology framework. Results This analysis uncovered four themes that can be mapped to disease management and the Chronic Care Model: (1) changing the health care system, (2) patient-centered care, (3) technological systems and barriers, and (4) integrating projects into the larger system. Project leaders discussed the paths, both direct and indirect, for transforming the health care system to one that addresses chronic illness. Patient-centered care was highlighted as needed and a paradigm shift for many. Challenges with technological systems were pervasive. Project leaders managed the expenses of a traveling technology, including the social, financial, and administration involved. Conclusions At the sites, project leaders served as travel guides, assisting and overseeing the programs as they traveled from the global plans to local actions. Project leaders, while hypothetically in control of the programs, in fact shared control of the traveling of the programs with patients, clinicians, and outside consultants. From this work, we can learn what roadblocks and expenses occur while a technology travels, from a project leader’s point of view. PMID:22578251

43 CFR 404.51 - Are proposed projects under the Rural Water Supply Program reviewed by the Administration?

Code of Federal Regulations, 2010 CFR

2010-10-01

... Water Supply Program reviewed by the Administration? 404.51 Section 404.51 Public Lands: Interior... SUPPLY PROGRAM Feasibility Studies § 404.51 Are proposed projects under the Rural Water Supply Program... the Reclamation's Rural Water Supply Program. This includes review under Executive Order 12322 to...

Coping in the Cyberworld: Program Implementation and Evaluation--A Pilot Project

ERIC Educational Resources Information Center

Lam, Cecilia Wing Chi; Frydenberg, Erica

2009-01-01

As increasing numbers of adolescents become involved in online activities, many also become victims of cyberharassment. This pilot project investigates how a program teaching coping skills (Best of Coping program, BOC) and a program teaching cybersafety (Cyber Savvy Teens program, CST) can optimise adolescents' capacity to cope online.…

7 CFR 1209.40 - Programs, plans, and projects.

Code of Federal Regulations, 2010 CFR

2010-01-01

... requirements for quality control, grade standards, supply management programs, or other programs that would..., plan, or project, no reference to a brand name, trade name, or State or regional identification of any...

A guide to federal-aid programs and projects

DOT National Transportation Integrated Search

1999-05-01

This guide provides basic information about Federal-aid programs, projects, and other program characteristics. The information sheets contained in the guide provide fiscal information, statutory and regulatory references, general eligibility and back...

Building Faculty Community: Fellowship in Graduate Medical Education Administration

PubMed Central

Edler, Alice A.; Dohn, Ann; Davidson, Heather A.; Grewal, Daisy; Behravesh, Bardia; Piro, Nancy

2009-01-01

Introduction The Department of Graduate Medical Education at Stanford Hospital and Clinics has developed a professional training program for program directors. This paper outlines the goals, structure, and expected outcomes for the one-year Fellowship in Graduate Medical Education Administration program. Background The skills necessary for leading a successful Accreditation Council for Graduate Medical Education (ACGME) training program require an increased level of curricular and administrative expertise. To meet the ACGME Outcome Project goals, program directors must demonstrate not only sophisticated understanding of curricular design but also competency-based performance assessment, resource management, and employment law. Few faculty-development efforts adequately address the complexities of educational administration. As part of an institutional-needs assessment, 41% of Stanford program directors indicated that they wanted more training from the Department of Graduate Medical Education. Intervention To address this need, the Fellowship in Graduate Medical Education Administration program will provide a curriculum that includes (1) readings and discussions in 9 topic areas, (2) regular mentoring by the director of Graduate Medical Education (GME), (3) completion of a service project that helps improve GME across the institution, and (4) completion of an individual scholarly project that focuses on education. Results The first fellow was accepted during the 2008–2009 academic year. Outcomes for the project include presentation of a project at a national meeting, internal workshops geared towards disseminating learning to peer program directors, and the completion of a GME service project. The paper also discusses lessons learned for improving the program. PMID:21975722

77 FR 31839 - Wind and Water Power Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-30

... DEPARTMENT OF ENERGY Office of Energy Efficiency and Renewable Energy Wind and Water Power Program... projects. The 2012 Wind and Water Power Program, Wind Power Peer Review Meeting will review wind technology development and market acceleration and deployment projects from the Program's research and development...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 23 Highways 1 2013-04-01 2013-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 23 Highways 1 2014-04-01 2014-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 23 Highways 1 2012-04-01 2012-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

7 CFR 1210.331 - Programs and projects.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., effectuation and administration of appropriate programs or projects for advertising and other sales promotion...; (d) A prohibition on advertising or other promotion programs that make any reference to private brand... PROMOTION PLAN Watermelon Research and Promotion Plan Research and Promotion § 1210.331 Programs and...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

Program theory-driven evaluation science in a youth development context.

PubMed

Deane, Kelsey L; Harré, Niki

2014-08-01

Program theory-driven evaluation science (PTDES) provides a useful framework for uncovering the mechanisms responsible for positive change resulting from participation in youth development (YD) programs. Yet it is difficult to find examples of PTDES that capture the complexity of such experiences. This article offers a much-needed example of PTDES applied to Project K, a youth development program with adventure, service-learning and mentoring components. Findings from eight program staff focus groups, 351 youth participants' comments, four key program documents, and results from six previous Project K research projects were integrated to produce a theory of change for the program. A direct logic analysis was then conducted to assess the plausibility of the proposed theory against relevant research literature. This demonstrated that Project K incorporates many of the best practice principles discussed in the literature that covers the three components of the program. The contributions of this theory-building process to organizational learning and development are discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

Columbia River Basin Fish and Wildlife Program Annual Implementation Work Plan for Fiscal Year 1992.

DOE Office of Scientific and Technical Information (OSTI.GOV)

United States. Bonneville Power Administration; Northwest Power Planning Council; Columbia Basin Fish and Wildlife Authority

1991-09-01

The Columbia River Basin Fish and Wildlife Program (Program) was developed by the Northwest Power Planning Council (Council) in accordance with Public Law 96-501, the Pacific Northwest Electric Power Planning and Conservation Act (Act). The purpose of the Program is to guide the Bonneville Power Administration (BPA) and other Federal agencies in carrying out their responsibilities to protect, mitigate, and enhance fish and wildlife of the Columbia River Basin. The Act explicitly gives BPA the authority and responsibility to use the BPA fund for these ends, to the extent that fish and wildlife are affected by the development and operationmore » of hydroelectric generation in the Columbia River Basin. The Columbia River Basin Fish and Wildlife Program Annual Implementation Work Plan (AIWP) presents BPA's plans for implementing the Program during Fiscal Year (FY) 1992. The AIWP reflects the primary goals of the Council's Action Plan (Section 1400 of the Program): to provide a solid, timely, and focused basis for budgeting and planning. In addition, the AIWP provides a means to judge the progress and the success of Program implementation. The AIWP is based on the outline developed by the Policy Review Group (PRG) during Step 1 of the annual cycle of the Implementation Planning Process (IPP), which is described in Section III. This AIWP has been organized and written to meet the specific needs of Program Action Items 10.1-10.3. The AIWP includes schedules with key milestones for FY 1992 and beyond, and addresses the Action Items assigned to BPA in Section 1400 of the 1987 Program and in subsequent amendments. All Program projects discussed in the AIWP are listed in Tables 1 and 2 according to their status as of May 21, 1991. Table 1 (pp. 3-14) lists completed, ongoing, and deferred projects. Table 2 (pp. 15-16) lists FY 1992 new-start projects. ''Ongoing'' status indicates that the project started in FY 1991 or before and that it is expected to continue through part or all of FY 1992. ''Deferred'' means that BPA implementation has been postponed to FY 1993 or later. ''Completed'' indicates completion during FY 1991. ''New'' denotes projects planned for BPA implementation in FY 1992. A number of projects are expected to begin in late FY 1991 and have been listed in Table 1 of the AIWP as ''Projected FY '91 Starts,'' based on their projected start dates. Several other projects are expected to end in late FY 1991. These projects have been listed in Table 1 as ''Projected FY '91 Completions,'' based on their projected completion dates.« less

Learning about the Earth through Societally-relevant Interdisciplinary Research Projects: the Honours Integrated Science Program at McMaster

NASA Astrophysics Data System (ADS)

Eyles, C.; Symons, S. L.; Harvey, C. T.

2016-12-01

Students in the Honours Integrated Science (iSci) program at McMaster University (Hamilton, Ontario, Canada) learn about the Earth through interdisciplinary research projects that focus on important societal issues. The iSci program is a new and innovative undergraduate program that emphasizes the links between scientific disciplines and focuses on learning through research and the development of scientific communication skills. The program accepts up to 60 students each year and is taught by a team of 18 instructors comprising senior and junior faculty, post-doctoral fellows, a lab coordinator, instructional assistant, a librarian and library staff, and an administrator. The program is designed around a pedagogical model that emphasizes hands-on learning through interdisciplinary research (Research-based Integrated Education: RIE) and is mostly project-based and experiential. In their freshman year students learn fundamental Earth science concepts (in conjunction with chemistry, physics, mathematics and biology) through research projects focused on environmental contamination, interplanetary exploration, the effect of drugs on the human body and environment, sustainable energy, and cancer. In subsequent years they conduct research on topics such as the History of the Earth, Thermodynamics, Plant-Animal Interactions, Wine Science, Forensics, and Climate Change. The iSci program attracts students with a broad interest in science and has been particularly effective in directing high quality students into the Earth sciences as they are introduced to the discipline in their first year of study through research projects that are interesting and stimulating. The structure of the iSci program encourages consideration of geoscientific applications in a broad range of societally relevant research projects; these projects are reviewed and modified each year to ensure their currency and ability to meet program learning objectives.

From Freshman Student to Upper-Secondary School Teacher in Chemistry: A New Approach with Projects and Group Work.

ERIC Educational Resources Information Center

Josephsen, Jens

1985-01-01

A special five and one-half year program for training upper secondary school chemistry teachers has been developed and tested over the past decade at Roskilde University near Copenhagen. The program (which emphasizes project work) and a student project on epoxy glue are described. Program graduates are generally problem-oriented and…

Alternative Basic Comprehension Program (Project A.B.C.). Final Evaluation Report, 1993-94. OER Report.

ERIC Educational Resources Information Center

Augustin, Marc A.; Yanping, Ann

The Alternative Basic Comprehension Program (Project A.B.C.) was an Elementary and Secondary Education Act Title VII-funded special alternative instructional program in its fourth year at two high schools in the Bronx (New York). In 1993-94, the project served 264 students of limited English proficiency from many countries. Participating students…

43 CFR 404.7 - What types of projects are eligible for consideration under the program?

Code of Federal Regulations, 2010 CFR

2010-10-01

... BUREAU OF RECLAMATION, DEPARTMENT OF THE INTERIOR RECLAMATION RURAL WATER SUPPLY PROGRAM Overview § 404.7... consideration under the program, a rural water supply project must: (a) Meet the definition of a rural water supply project in § 404.2; and (b) Be located in a Reclamation State, as defined in § 404.2. ...

International Service Learning and Short-Term Business Study Abroad Programs: A Case Study

ERIC Educational Resources Information Center

Le, Quan V.; Raven, Peter V.; Chen, Stanley

2013-01-01

A service learning project was recently incorporated into a short-term business study abroad program. The main objective was to assess whether there is a place for service learning projects and how they should be integrated into the program. A combination of two surveys were used, one taken before the project and one after. Reflection papers were…

34 CFR 379.11 - What additional types of project activities may be authorized under this program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What additional types of project activities may be... Program? § 379.11 What additional types of project activities may be authorized under this program? The... requirements of the ADA, as that Act relates to employment of individuals with disabilities. (Authority: 29 U.S...

25 CFR 170.623 - How are IRR Program projects and activities included in a self-governance agreement?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false How are IRR Program projects and activities included in a self-governance agreement? 170.623 Section 170.623 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE... self-governance agreement? To include an IRR Program project or activity in a self-governance agreement...

Space Science Research and Technology at NASA's Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

Johnson, Charles L.

2007-01-01

This presentation will summarize the various projects and programs managed in the Space Science Programs and Projects Office at NASA's Marshall Space Flight Center in Huntsville, Alabama. Projects in the portfolio include NASA's Chandra X-Ray telescope, Hinode solar physics satellite, various advanced space propulsion technologies, including solar sails and tethers, as well as NASA's Discovery and New Frontiers Programs.

Starfleet Deferred: Project Orion in the 1962 Air Force Space Program

NASA Astrophysics Data System (ADS)

Ziarnick, B.

Project Orion, the Cold War American program (1957-1965) studying nuclear pulse propulsion for space applications, has long interested space enthusiasts for what it was and what it might have been, but it has long been believed that neither the United States government nor the US Air Force took the program very seriously. However, recently declassified US Air Force documents shed more light on the classified history of Project Orion. Far from being ignored by Air Force leadership, through the efforts of the Strategic Air Command, Air Force leaders like General Curtis LeMay were convinced that Project Orion should be funded as a major weapons system. The high water mark of Project Orion was the 1962 Air Force Space Program proposal by the Air Force Chief of Staff to devote almost twenty percent of the Air Force space budget from 1962-1967 to Orion development before the program was cancelled by the civilian Secretary of the Air Force under pressure from the Department of Defense. This paper details the history of Project Orion in the 1962 Air Force Space Program proposal, and concludes with a few lessons learned for use by modern interstellar advocates.

Service learning at dementia care programs: an orientation and training program.

PubMed

Lambert-Shute, Jennifer J; Jarrott, Shannon E; Fruhauf, Christine A

2004-01-01

The present paper describes a project that addresses the unique challenge service-learners face at dementia care programs. The project was conducted in conjunction with two courses on aging that offer students a service-learning (S-L) option at a university adult day service (ADS) program that accepts service-learners from these courses. The intervention consisted of two elements: (a) an orientation and follow- up sessions in which students learned about the causes of dementia, the behavioral characteristics exhibited by persons with dementia, and the best methods of interacting effectively with cognitively impaired persons; and (b) a structured scrapbook project for service-learners to exercise their new knowledge and skills. The authors designed the project to increase students' comfort in the dementia care setting and to facilitate students' knowledge of the elderly. The following sections describe the project and its procedures, benefits, and challenges. Recommendations for continuing the project and for adapting the project to other dementia sites are provided.

Mobile-Based Nutrition and Child Health Monitoring to Inform Program Development: An Experience From Liberia.

PubMed

Guyon, Agnes; Bock, Ariella; Buback, Laura; Knittel, Barbara

2016-12-23

Implementing complex nutrition and other public health projects and tracking nutrition interventions, such as women's diet and supplementation and infant and young child feeding practices, requires reliable routine data to identify potential program gaps and to monitor trends in behaviors in real time. However, current monitoring and evaluation practices generally do not create an environment for this real-time tracking. This article describes the development and application of a mobile-based nutrition and health monitoring system, which collected monitoring data on project activities, women's nutrition, and infant and young child feeding practices in real time. The Liberia Agricultural Upgrading Nutrition and Child Health (LAUNCH) project implemented a nutrition and health monitoring system between April 2012 and June 2014. The LAUNCH project analyzed project monitoring and outcome data from the system and shared selected behavioral and programmatic indicators with program managers through a short report, which later evolved into a visual data dashboard, during program-update meetings. The project designed protocols to ensure representativeness of program participants. LAUNCH made programmatic adjustments in response to findings from the monitoring system; these changes were then reflected in subsequent quarterly trends, indicating that the availability of timely data allowed for the project to react quickly to issues and adapt the program appropriately. Such issues included lack of participation in community groups and insufficient numbers of food distribution points. Likewise, the system captured trends in key outcome indicators such as breastfeeding and complementary feeding practices, linking them to project activities and external factors including seasonal changes and national health campaigns. Digital data collection platforms can play a vital role in improving routine programmatic functions. Fixed gathering locations such as food distribution points represent an opportunity to easily access program participants and enable managers to identify strengths and weaknesses in project implementation. For programs that track individuals over time, a mobile tool combined with a strong database can greatly improve efficiency and data visibility and reduce resource leakages. © Guyon et al.

NASA High Performance Computing and Communications program

NASA Technical Reports Server (NTRS)

Holcomb, Lee; Smith, Paul; Hunter, Paul

1994-01-01

The National Aeronautics and Space Administration's HPCC program is part of a new Presidential initiative aimed at producing a 1000-fold increase in supercomputing speed and a 1(X)-fold improvement in available communications capability by 1997. As more advanced technologies are developed under the HPCC program, they will be used to solve NASA's 'Grand Challenge' problems, which include improving the design and simulation of advanced aerospace vehicles, allowing people at remote locations to communicate more effectively and share information, increasing scientists' abilities to model the Earth's climate and forecast global environmental trends, and improving the development of advanced spacecraft. NASA's HPCC program is organized into three projects which are unique to the agency's mission: the Computational Aerosciences (CAS) project, the Earth and Space Sciences (ESS) project, and the Remote Exploration and Experimentation (REE) project. An additional project, the Basic Research and Human Resources (BRHR) project, exists to promote long term research in computer science and engineering and to increase the pool of trained personnel in a variety of scientific disciplines. This document presents an overview of the objectives and organization of these projects, as well as summaries of early accomplishments and the significance, status, and plans for individual research and development programs within each project. Areas of emphasis include benchmarking, testbeds, software and simulation methods.

Columbia River Basin Fish and Wildlife Program Work Plan for Fiscal Year 1989.

DOE Office of Scientific and Technical Information (OSTI.GOV)

United States. Bonneville Power Administration. Division of Fish and Wildlife.

1988-11-01

The FY 1989 Columbia River Basin Fish and Wildlife Program Work Plan (Work Plan) presents Bonneville Power Administration's plans for implementing the Columbia River Basin Fish and Wildlife Program (Program) in FY 1989. The Work Plan focuses on individual Action Items found in the 1987 Program for which Bonneville Power Administration (BPA) has determined that it has authority and responsibility to implement. Each of the entries in the Work Plan includes objectives, background, and progress to date in achieving those objectives, and a summary of plans for implementation in FY 1989. Most Action Items are implemented through one or moremore » BPA-funded projects. Each Action Item entry is followed by a list of completed, ongoing, and planned projects, along with objectives, results, schedules, and milestones for each project. The FY 1989 Work Plan emphasizes continuation of 113 projects, most of which involve protection, mitigation, or enhancement of anadromous fishery resources. BPA also plans to start 20 new projects in FY 1989. The number of ongoing FY 1988 projects to be continued in FY 1989 and the number of new projects planned to start in FY 1989 are based on current (September 7, 1988) procurement expectations. Several projects presently in BPA's procurement process are expected to be contracted by September 30, 1988, the last day of FY 1988. Although these projects have not yet started, they have been listed in the Work Plan as ongoing FY 1988 projects, based on projected start dates in late September 1988. Throughout the Work Plan, those projects with projected start dates in September 1988 have been noted.« less

24 CFR 5.653 - Section 8 project-based assistance programs: Admission-Income-eligibility and income-targeting.

Code of Federal Regulations, 2010 CFR

2010-04-01

... assistance programs, except for the moderate rehabilitation and the project-based certificate or voucher... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Section 8 project-based assistance... Disabilities: Family Income and Family Payment; Occupancy Requirements for Section 8 Project-Based Assistance...

24 CFR 5.653 - Section 8 project-based assistance programs: Admission-Income-eligibility and income-targeting.

Code of Federal Regulations, 2011 CFR

2011-04-01

... assistance programs, except for the moderate rehabilitation and the project-based certificate or voucher... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Section 8 project-based assistance... Disabilities: Family Income and Family Payment; Occupancy Requirements for Section 8 Project-Based Assistance...

34 CFR 359.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2012 CFR

2012-07-01

... 34 Education 2 2012-07-01 2012-07-01 false What types of projects are authorized under this... Does the Secretary Assist Under This Program? § 359.10 What types of projects are authorized under this... clinical research and the analysis of standardized data in collaboration with other related projects...

The Monterey Model for Micro Business Special Education

ERIC Educational Resources Information Center

Moberg, Eric M.

2009-01-01

The following program description, literature review, and theoretical bases demonstrate the andragogical soundness of the two projects and a club of the Monterey Adult Transitions Program. The Bistro Project began as a suggestion of an Associate Superintendent, who ran a similar project in the Boston area. The Greenhouse Project began as a…

Project Tradition and Technology (Project TNT): The Hualapai Bilingual Academic Excellence Program.

ERIC Educational Resources Information Center

Reed, Michael D.; And Others

Project Tradition and Technology (TNT) at Peach Springs Elementary School (Peach Springs, Arizona) is 1 of 12 programs recognized nationally as an outstanding model of bilingual education by the U.S. Department of Education. Project TNT is a process-oriented curriculum development model that identifies the community's needs and expectations for…

2+2+2 Dissemination Project Final Report.

ERIC Educational Resources Information Center

Sloane, Diana; And Others

An overview is provided in this three-part final report of a project designed to disseminate information to assist California high schools, Regional Occupation Programs, and colleges in developing and strengthening 2+2+2 programs. Part I reviews the following project objectives: (1) hire a project coordinator and technical assistant; (2) develop a…

Research-Informed Curriculum Design for a Master's-Level Program in Project Management

ERIC Educational Resources Information Center

Bentley, Yongmei; Richardson, Diane; Duan, Yanqing; Philpott, Elly; Ong, Vincent; Owen, David

2013-01-01

This article reports on the application of Research-Informed Curriculum Design (RICD) for the development and implementation of an MSc Program in Project Management. The research focused on contemporary issues in project management and provided an analysis of project management approaches, tools, and techniques currently used in organizations.…

Hawaii English Program: Project End Evaluation Report 1970-1971.

ERIC Educational Resources Information Center

Hawaii State Dept. of Education, Honolulu.

This report is comprised of two reports: the Final Audit Report of the Hawaii English Project, submitted by the Northwest Regional Educational Laboratory, and the main report, the Hawaii English Program Project End Evaluation Report by the Hawaii English Project Staff. The Audit Report is limited to a review of data reduction, analysis, and…

Federal Programs Supporting Educational Change, Vol. 2: Factors Affecting Change Agent Projects.

ERIC Educational Resources Information Center

Berman, Paul; Pauly, Edward W.

This second volume in the change-agent series reports the interim results of an exploratory statistical analysis of a survey of a nationwide sample of 293 change-agent projects funded by four federal demonstration programs--Elementary Secondary Education Act (ESEA) Title III, Innovative Projects; ESEA Title VII, Bilingual Projects; Vocational…

10 CFR 611.102 - Eligible project costs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Eligible project costs. 611.102 Section 611.102 Energy... PROGRAM Direct Loan Program § 611.102 Eligible project costs. (a) Eligible costs are: (1) Those costs that.... (b) In determining the overall total cost of an Eligible Project, DOE and the applicant may include...

10 CFR 611.102 - Eligible project costs.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Eligible project costs. 611.102 Section 611.102 Energy... PROGRAM Direct Loan Program § 611.102 Eligible project costs. (a) Eligible costs are: (1) Those costs that.... (b) In determining the overall total cost of an Eligible Project, DOE and the applicant may include...

10 CFR 611.102 - Eligible project costs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Eligible project costs. 611.102 Section 611.102 Energy... PROGRAM Direct Loan Program § 611.102 Eligible project costs. (a) Eligible costs are: (1) Those costs that.... (b) In determining the overall total cost of an Eligible Project, DOE and the applicant may include...

10 CFR 611.102 - Eligible project costs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Eligible project costs. 611.102 Section 611.102 Energy... PROGRAM Direct Loan Program § 611.102 Eligible project costs. (a) Eligible costs are: (1) Those costs that.... (b) In determining the overall total cost of an Eligible Project, DOE and the applicant may include...

34 CFR 371.10 - What types of projects are authorized under this program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false What types of projects are authorized under this... REHABILITATION SERVICE PROJECTS FOR AMERICAN INDIANS WITH DISABILITIES What Kinds of Activities Does the Department of Education Assist Under This Program? § 371.10 What types of projects are authorized under this...

34 CFR 664.1 - What is the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2010 CFR

2010-07-01

... Group Projects Abroad Program is designed to contribute to the development and improvement of the study... 34 Education 3 2010-07-01 2010-07-01 false What is the Fulbright-Hays Group Projects Abroad... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD...

34 CFR 664.1 - What is the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2011 CFR

2011-07-01

... Group Projects Abroad Program is designed to contribute to the development and improvement of the study... 34 Education 3 2011-07-01 2011-07-01 false What is the Fulbright-Hays Group Projects Abroad... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD...

34 CFR 664.1 - What is the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2014 CFR

2014-07-01

... Group Projects Abroad Program is designed to contribute to the development and improvement of the study... 34 Education 3 2014-07-01 2014-07-01 false What is the Fulbright-Hays Group Projects Abroad... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD...

34 CFR 664.1 - What is the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2012 CFR

2012-07-01

... Group Projects Abroad Program is designed to contribute to the development and improvement of the study... 34 Education 3 2012-07-01 2012-07-01 false What is the Fulbright-Hays Group Projects Abroad... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD...

34 CFR 664.1 - What is the Fulbright-Hays Group Projects Abroad Program?

Code of Federal Regulations, 2013 CFR

2013-07-01

... Group Projects Abroad Program is designed to contribute to the development and improvement of the study... 34 Education 3 2013-07-01 2013-07-01 false What is the Fulbright-Hays Group Projects Abroad... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FULBRIGHT-HAYS GROUP PROJECTS ABROAD...

Project MOVE Program Planning Kit.

ERIC Educational Resources Information Center

Farris, Charlotte J.

This program planning kit contains information and activities on evaluation, change, leadership, and sex stereotyping which provided the basis for planning Project MOVE (Maximizing Options in Vocational Education) programs but which can be generalized and adapted for use in planning other programs. Assumptions ano guidelines based upon theory and…

Competency-Based Adult Education Research and Innovation Catalog.

ERIC Educational Resources Information Center

Bureau of Occupational and Adult Education (DHEW/OE), Washington, DC. Div. of Adult Education.

This document contains abstracts describing competency-based programs related to the Adult Performance Level (APL) project and adult education. The catalog is divided into two sections: state program implementation and local program implementation. The first section describes programs and research projects currently active in Alabama, California,…

Projection of Teachers' Salaries for Contract Negotiations.

ERIC Educational Resources Information Center

Ott, Jack P.

1982-01-01

Lists and explains a computer program written in BASIC which calculates teacher salaries using a salary index. Modification of this payroll program is suggested as a student project in schools which teach computer programing. (JJD)

Idaho Transportation Department : 2010 research program peer exchange.

DOT National Transportation Integrated Search

2010-05-01

The objectives of the peer exchange were to: : 1. Identify strengths, challenges, and opportunities for program and project management; : 2. Understand management expectations of the ITD Research Program; : 3. Review processes for project selection a...

Issues in NASA Program and Project Management. Special Report: 1997 Conference. Project Management Now and in the New Millennium

NASA Technical Reports Server (NTRS)

Hoffman, Edward J. (Editor); Lawbaugh, William M. (Editor)

1997-01-01

Topics Considered Include: NASA's Shared Experiences Program; Core Issues for the Future of the Agency; National Space Policy Strategic Management; ISO 9000 and NASA; New Acquisition Initiatives; Full Cost Initiative; PM Career Development; PM Project Database; NASA Fast Track Studies; Fast Track Projects; Earned Value Concept; Value-Added Metrics; Saturn Corporation Lessons Learned; Project Manager Credibility.

Project Manager Personality as a Factor for Success

DTIC Science & Technology

2008-03-01

the project manager includes planning , 1 organizing, directing, and controlling the project (Program Management Institute, 2004; Kerzner, 2006). By...programs identified 5 percent cost growth per year from 2004 to 2007, costing $165 billion dollars more than originally planned (United States...Bjeirmi, 1996) and point out that good project managers can "contribute to project success, but are unlikely to prevent failure" ( de Witt, 1988, p