Exploring abundance, diversity and variation of a widespread antibiotic resistance gene in wastewater treatment plants.

PubMed

Wei, Ziyan; Feng, Kai; Li, Shuzhen; Zhang, Yu; Chen, Hongrui; Yin, Huaqun; Xu, Meiying; Deng, Ye

2018-05-09

An updated sul1 gene sequence database was constructed and new degenerate primers were designed to better investigate the abundance, diversity, and variation of a ubiquitous antibiotic resistance gene, sul1, with PCR-based methods in activated sludge from wastewater treatment plants (WWTPs). The newly designed degenerate primers showed high specificity and higher coverage in both in-silico evaluations and activated sludge samples compared to previous sul1 primers. Using the new primers, the abundance and diversity of sul1 gene, together with 16S rRNA gene, in activated sludge from five WWTPs in summer and winter were determined by quantitative PCR and MiSeq sequencing. The sul1 gene was found to be prevalent and displayed a comparable abundance (0.081 copies per bacterial cell in average) to the total bacteria across all samples. However, compared to the significant seasonal and geographical divergences in the quantity and diversity of bacterial communities in WWTPs, there were no significant seasonal or geographical variations of representative clusters of sul1 gene in most cases. Additionally, the representative sul1 clusters showed fairly close phylogeny and there was no obvious correlation between sul1 gene and the dominant bacterial genera, as well as the int1 gene, suggesting that bacterial hosts of sul1 gene is not stable, the sul1 gene may be carried by mobile genetic elements, sometimes integrated with class 1 integrons and sometimes not. Thus mobile genetic elements likely play a greater role than specific microbial taxa in determining the composition of sul1 gene in WWTPs. Copyright © 2018. Published by Elsevier Ltd.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takei, Masao; Nakagawa, Hideyuki

The sea urchin Toxopneustes pileolus belonging to the family Toxopneustidae, they have well-developed globiferous pedicellariae with pharmacologically active substances. We have purified a novel sea urchin lectin-1 (SUL-1) from the large globiferous pedicellariae of T. pileolus. Dendritic cells (DC) are professional APC and play a pivotal role in controlling immune responses. This study investigated whether SUL-1 can drive DC maturation from human immature monocyte-derived DC in vitro. Human monocytes were cultured with GM-CSF and IL-4 for 6 days followed by another 1 day in the presence of SUL-1 or LPS. DC harvested on day 7 were examined using functional assays.more » The expression levels of CD1a, CD80, CD83, CD86 and HLA-DR as expressed by mean fluorescence intensity (MFI) on DC differentiated from immature DC after culture with 1.0 {mu}g/ml of SUL-1 for 1 day were enhanced and decreased endocytic activity. SUL-1-treated DC also displayed enhanced T cell stimulatory capacity in an MLR, as measured by T cell proliferation. Cell surface expression of CD80, CD83 and CD86 on SUL-1-treated DC was inhibited by anti-DC-SIGN mAb, while anti-DC-SIGN mAb had no influence on allogeneic T cell proliferation by SUL-1-treated DC. DC differentiated with SUL-1 induced the differentiation of naive T cell towards a helper T cell type 1 (Th1) response at DC/T (1:5) cells ratio depending on IL-12 secretion. In CTL assay, the production of IFN-{gamma} and {sup 51}Cr release on SUL-1-treated DC were more augmented than of immature DC or LPS-treated DC. SUL-1-treated DC expressed CCR7 and had a high migration to MIP-3{beta}. Intracellular Ca{sup 2+} mobilization in SUL-1-treated DC was also induced by MIP-3{beta}. These results suggest that SUL-1 bindings to DC-SIGN on surface of immature DC may lead to differentiate DC from immature DC. Moreover, it suggests that SUL-1 may be used on DC-based vaccines for cancer immunotherapy.« less

Dissemination of Trimethoprim-Sulfamethoxazole Drug Resistance Genes Associated with Class 1 and Class 2 Integrons Among Gram-Negative Bacteria from HIV Patients in South India.

PubMed

Ramesh Kumar, Marimuthu Ragavan; Arunagirinathan, Narasingam; Srivani, Seetharaman; Dhanasezhian, Aridoss; Vijaykanth, Nallusamy; Manikandan, Natesan; Balakrishnan, Sethuramalingam; Vignesh, Ramachandran; Balakrishnan, Pachamuthu; Solomon, Suniti; Solomon, Sunil S

2017-07-01

The antibiotic, trimethoprim-sulfamethoxazole (TMP-SMX), is generally used for prophylaxis in HIV individuals to protect them from Pneumocystis jiroveci infection. Long-term use of TMP-SMX develops drug resistance among bacteria in HIV patients. The study was aimed to detect the TMP-SMX resistance genes among gram-negative bacteria from HIV patients. TMP-SMX-resistant isolates were detected by the Kirby-Bauer disc diffusion method. While TMP resistance genes such as dfrA1, dfrA5, dfrA7, and dfrA17 and SMX resistance genes such as sul1 and sul2 were detected by multiplex PCR, class 1 and class 2 integrons were detected by standard monoplex PCR. Of the 151 TMP-SMX-resistant bacterial isolates, 3 were positive for sul1 alone, 48 for sul2 alone, 11 for dfrA7 alone, 21 for sul1 and sul2, 1 for sul1 and dfrA7, 23 for sul2 and dfrA7, 2 for sul2 and dfrA5, 41 for sul1, sul2, and dfrA7, and 1 for sul2, dfrA5, and dfrA7. Of 60 TMP-SMX-resistant isolates positive for integrons, 44 had class 1 and 16 had class 2 integrons. It was found that the prevalence of sul genes (n = 202; p < 0.001) was higher compared with dfr genes (n = 80; p < 0.001), and 87.4% (n = 132; p < 0.001) of TMP-SMX-resistant isolates also were positive for β-lactamase production. This type of study is reported for the first time from HIV patients in India. Therefore, this study indicates that dissemination of TMP-SMX resistance genes and class 1 and class 2 integrons along with β-lactamase production among gram-negative bacteria in HIV patients will certainly make their treatment to bacterial infections more complicated in clinical settings.

A new sulfonamide resistance gene (sul3) in Escherichia coli is widespread in the pig population of Switzerland.

PubMed

Perreten, Vincent; Boerlin, Patrick

2003-03-01

A new gene, sul3, which specifies a 263-amino-acid protein similar to a dihydropteroate synthase encoded by the 54-kb conjugative plasmid pVP440 from Escherichia coli was characterized. Expression of the cloned sul3 gene conferred resistance to sulfamethoxazole on E. coli. Two copies of the insertion element IS15Delta/26 flanked the region containing sul3. The sul3 gene was detected in one-third of the sulfonamide-resistant pathogenic E. coli isolates from pigs in Switzerland.

A New Sulfonamide Resistance Gene (sul3) in Escherichia coli Is Widespread in the Pig Population of Switzerland

PubMed Central

Perreten, Vincent; Boerlin, Patrick

2003-01-01

A new gene, sul3, which specifies a 263-amino-acid protein similar to a dihydropteroate synthase encoded by the 54-kb conjugative plasmid pVP440 from Escherichia coli was characterized. Expression of the cloned sul3 gene conferred resistance to sulfamethoxazole on E. coli. Two copies of the insertion element IS15Δ/26 flanked the region containing sul3. The sul3 gene was detected in one-third of the sulfonamide-resistant pathogenic E. coli isolates from pigs in Switzerland. PMID:12604565

Sulphursoil - Delano Development Corporation

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1985-06-01

A sizable amount of technical information has been accumulated on the effects of agricultural applications of the natural mineral product called Sul-Fe. This technical information supports the field observations of farmers, landscapers and gardeners who have used the product. Sul-Fe is often evaluated in terms of its sulphur content alone. When compared to elemental sulphur (100% sulphur), the 18 to 21% sulphur content of Sul-Fe seems relatively low. However, as the following technical data indicates, when judged on actual effects, Sul-Fe's complex mixture of minerals has several advantages over elemental sulphur. When judged on the basis of soil acidulation, Sul-Femore » has more immediate effects than elemental sulphur. The rapid acidifying effect is due to Sul-Fe's content of crystalline sulphuric acid. Sul-Fe also has long-term effects on soil pH due to its content of sulphur and sulfides and the time required to oxidize these materials. Elemental sulphur contains sulphur in only one chemical form which must be microbially oxidized before it becomes reactive in the soil solution, a reaction that takes quite some time in some soils. Sul-Fe is thus better than elemental sulphur in terms of immediate effects and comparable in terms of long term effects. Applied blends of Sul-Fe supplemented with elemental sulphur may provide for a maximization of both short and long term effects. An additional benefit derived from the use of Sul-Fe is the addition to the soil of a variety of trace nutrients including iron, calcium, zinc, copper, manganese, magnesium, and molybdenum.« less

Effects of corresponding and non-corresponding contaminants on the fate of sulfonamide and quinolone resistance genes in the Laizhou Bay, China.

PubMed

Li, Qianwei; Na, Guangshui; Zhang, Linxiao; Lu, Zihao; Gao, Hui; Li, Ruijing; Jin, Shuaichen

2018-03-01

The environmental behaviors and migration patterns of antibiotic resistance genes (ARGs) have attracted considerable research interest. However, there has been little research concerning the effects of corresponding and non-corresponding contaminants on the fate of ARGs in coastal environments. In the present study, the distribution of intI1, sul1, sul2, qnrS and aac(6')-Ib were analyzed in water and sediment samples of Laizhou Bay in the context of corresponding and non-corresponding contaminants. The abundance of intI1, sul1 and sul2 genes exhibited a clear decreasing trend extending from the inner estuary to the coastal area. Strong and positive correlations existed between sul1/sul2 and sulfonamide antibiotic residues in sediments, and between the abundances of intI1 and sul1/sul2. Statistical analyses indicated that non-corresponding contaminants were partially correlated with ARG abundances. These results suggest that non-corresponding contaminants may have direct or indirect influences on the abundances of ARGs and intI1 in the Laizhou Bay. Copyright © 2018 Elsevier Ltd. All rights reserved.

The 6-hydroxychromanol derivative SUL-109 ameliorates renal injury after deep hypothermia and rewarming in rats.

PubMed

Vogelaar, Pieter C; Roorda, Maurits; de Vrij, Edwin L; Houwertjes, Martin C; Goris, Maaike; Bouma, Hjalmar; van der Graaf, Adrianus C; Krenning, Guido; Henning, Robert H

2018-04-11

Mitochondrial dysfunction plays an important role in kidney damage in various pathologies, including acute and chronic kidney injury and diabetic nephropathy. In addition to the well-studied ischaemia/reperfusion (I/R) injury, hypothermia/rewarming (H/R) also inflicts acute kidney injury. Substituted 6-hydroxychromanols are a novel class of mitochondrial medicines that ameliorate mitochondrial oxidative stress and protect the mitochondrial network. To identify a novel 6-hydroxychromanol that protects mitochondrial structure and function in the kidney during H/R, we screened multiple compounds in vitro and subsequently assessed the efficacy of the 6-hydroxychromanol derivatives SUL-109 and SUL-121 in vivo to protect against kidney injury after H/R in rats. Human proximal tubule cell viability was assessed following exposure to H/R for 48/4 h in the presence of various 6-hydroxychromanols. Selected compounds (SUL-109, SUL-121) or vehicle were administered to ketamine-anaesthetized male Wistar rats (IV 135 µg/kg/h) undergoing H/R at 15°C for 3 h followed by rewarming and normothermia for 1 h. Metabolic parameters and body temperature were measured throughout. In addition, renal function, renal injury, histopathology and mitochondrial fitness were assessed. H/R injury in vitro lowered cell viability by 94 ± 1%, which was counteracted dose-dependently by multiple 6-hydroxychomanols derivatives. In vivo, H/R in rats showed kidney injury molecule 1 expression in the kidney and tubular dilation, accompanied by double-strand DNA breaks and protein nitrosylation. SUL-109 and SUL-121 ameliorated tubular kidney damage, preserved mitochondrial mass and maintained cortical adenosine 5'-triphosphate (ATP) levels, although SUL-121 did not reduce protein nitrosylation. The substituted 6-hydroxychromanols SUL-109 and SUL-121 ameliorate kidney injury during in vivo H/R by preserving mitochondrial mass, function and ATP levels. In addition, both 6-hydroxychromanols limit DNA damage, but only SUL-109 also prevented protein nitrosylation in tubular cells. Therefore SUL-109 offers a promising therapeutic strategy to preserve kidney mitochondrial function.

SulE, a Sulfonylurea Herbicide De-Esterification Esterase from Hansschlegelia zhihuaiae S113

PubMed Central

Hang, Bao-Jian; Hong, Qing; Xie, Xiang-Ting; Huang, Xing; Wang, Cheng-Hong; Li, Shun-Peng

2012-01-01

De-esterification is an important degradation or detoxification mechanism of sulfonylurea herbicide in microbes and plants. However, the biochemical and molecular mechanisms of sulfonylurea herbicide de-esterification are still unknown. In this study, a novel esterase gene, sulE, responsible for sulfonylurea herbicide de-esterification, was cloned from Hansschlegelia zhihuaiae S113. The gene contained an open reading frame of 1,194 bp, and a putative signal peptide at the N terminal was identified with a predicted cleavage site between Ala37 and Glu38, resulting in a 361-residue mature protein. SulE minus the signal peptide was synthesized in Escherichia coli BL21 and purified to homogeneity. SulE catalyzed the de-esterification of a variety of sulfonylurea herbicides that gave rise to the corresponding herbicidally inactive parent acid and exhibited the highest catalytic efficiency toward thifensulfuron-methyl. SulE was a dimer without the requirement of a cofactor. The activity of the enzyme was completely inhibited by Ag+, Cd2+, Zn2+, methamidophos, and sodium dodecyl sulfate. A sulE-disrupted mutant strain, ΔsulE, was constructed by insertion mutation. ΔsulE lost the de-esterification ability and was more sensitive to the herbicides than the wild type of strain S113, suggesting that sulE played a vital role in the sulfonylurea herbicide resistance of the strain. The transfer of sulE into Saccharomyces cerevisiae BY4741 conferred on it the ability to de-esterify sulfonylurea herbicides and increased its resistance to the herbicides. This study has provided an excellent candidate for the mechanistic study of sulfonylurea herbicide metabolism and detoxification through de-esterification, construction of sulfonylurea herbicide-resistant transgenic crops, and bioremediation of sulfonylurea herbicide-contaminated environments. PMID:22247165

A 90-day subchronic toxicity study with sodium formononetin-3'-sulphonate (Sul-F) delivered to dogs via intravenous administration.

PubMed

Li, Chunmei; Li, Guisheng; Gao, Yonglin; Sun, Chengfeng; Wang, Xiaoyan

2016-06-01

Sodium formononetin-3'-sulphonate (Sul-F) is a water-soluble derivate of formononetin, and an increasing number of studies have shown that Sul-F not only possesses favorable water solubility but also exhibits good lipid-lowering and bioactivities. In the current study, the toxicity of Sul-F was evaluated in dogs after 90-day intravenous infusion. Dogs were treated with Sul-F at dose of 0, 33.3, 100, and 300 mg/kg, and observed for 90-day followed by 28-day recovery period. Weekly measurement of body weight, temperature and food consumption were conducted. Ophthalmoscopy, ECG examination, urinalysis, serum biochemistry and hematology examination were performed at pre-test, on days 45 and 90, and following by 28-day recovery period. Histological examination was performed on day 90 and 28-day recovery period. No mortality, ophthalmic abnormalities or treatment-related findings in body weight, clinical chemistry, hematology, and histopathological examination were detected. However, a white crystal (non-metabolic Sul-F), transient vomiting and recoverable vascular stimulation were observed in 300 mg/kg/day Sul-F treated dogs. Under the conditions, the no-observed-adverse-effect-level (NOAEL) for Sul-F was 100 mg/kg in dogs. Copyright © 2016 Elsevier Inc. All rights reserved.

Evolution of corresponding resistance genes in the water of fish tanks with multiple stresses of antibiotics and heavy metals.

PubMed

He, Xiaolin; Xu, Yanbin; Chen, Jinliang; Ling, Jiayin; Li, Yafei; Huang, Lu; Zhou, Xiao; Zheng, Li; Xie, Guangyan

2017-11-01

Abuse of antibiotics and heavy metals in aquaculture has been widely concerned and might aggravate the spread of resistance genes in environment. To investigate the occurrence and proliferation of antibiotic resistance genes (ARGs) and heavy metal resistance genes (HMRGs), three commonly used antibiotics (tetracycline, sulfanilamide, cefotaxime) and two heavy metals (Zn and Cu) were designed to add individually or jointly in nine fish tanks including five individual exposure tanks of tetracycline (tet), sulfanilamide (sul), cefotaxime (cef), Cu, Zn and four combination exposure tanks of tetracycline + sulfanilamide (tet + sul), tetracycline + sulfanilamide + cefotaxime (tet + sul + cef), tetracycline + sulfanilamide + Cu (tet + sul + Cu), tetracycline + sulfanilamide + Zn (tet + sul + Zn) as well as the control during the experiment period of 180 days. Nineteen ARGs (tetA, tetB, tetC, tetD, tetE, tetG, tetM, tetO, tetQ, tetS, tetW, tetX, tetY, sul1, sul2, sul3, bla DHA , bla MOX , bla FOX ), two HMRGs (copA, czcA) and the class 1 integron gene (intI 1) in fish tanks water were investigated. The results showed that the residual rate of antibiotics and heavy metals ranged from 0.03% to 2.46% and 9.25%-52.97%, respectively, positively related to their original concentration and types. Tetracycline resistance genes were more sensitive to antibiotics and easier to be induced and developed than sulfanilamide resistance genes and AmpC β-lactamase resistance genes. The total relative abundances of ARGs in combined stresses exposure tanks (tet + sul, tet + sul + cef, tet + sul + Cu, tet + sul + Zn) were about 1.01-1.55 times more than the sum of their individual ones. The co-selective effects of cefotaxime on the abundance and diversity of tetracycline resistance genes were stronger than Zn and Cu. Besides, multivariate correlation analysis revealed that tetO, tetQ, tetW and sul3 were in significant correlation with the concentrations of Cu and Zn (r = 0.882-0.992, p < 0.05 or p < 0.01). The significant correlations between tetO and intI1 (p < 0.01), tetW and intI1 (p < 0.05), and sul3 and intI1 (p < 0.05) hinted a potentially serious and undesirable dissemination risk of ribosomal protection proteins gene of ARGs. Copyright © 2017 Elsevier Ltd. All rights reserved.

Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus.

PubMed

Hatakeyama, Tomomitsu; Ichise, Ayaka; Unno, Hideaki; Goda, Shuichiro; Oda, Tatsuya; Tateno, Hiroaki; Hirabayashi, Jun; Sakai, Hitomi; Nakagawa, Hideyuki

2017-08-01

The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contains several biologically active proteins. We have cloned the cDNA of one of the toxin components, SUL-I, which is a rhamnose-binding lectin (RBL) that acts as a mitogen through binding to carbohydrate chains on target cells. Recombinant SUL-I (rSUL-I) was produced in Escherichia coli cells, and its carbohydrate-binding specificity was examined with the glycoconjugate microarray analysis, which suggested that potential target carbohydrate structures are galactose-terminated N-glycans. rSUL-I exhibited mitogenic activity for murine splenocyte cells and toxicity against Vero cells. The three-dimensional structure of the rSUL-I/l-rhamnose complex was determined by X-ray crystallographic analysis at a 1.8 Å resolution. The overall structure of rSUL-I is composed of three distinctive domains with a folding structure similar to those of CSL3, a RBL from chum salmon (Oncorhynchus keta) eggs. The bound l-rhamnose molecules are mainly recognized by rSUL-I through hydrogen bonds between its 2-, 3-, and 4-hydroxy groups and Asp, Asn, and Glu residues in the binding sites, while Tyr and Ser residues participate in the recognition mechanism. It was also inferred that SUL-I may form a dimer in solution based on the molecular size estimated via dynamic light scattering as well as possible contact regions in its crystal structure. © 2017 The Protein Society.

Nanostructured Ceramics Obtained Under Extreme Conditions of Pressure and Temperature

DTIC Science & Technology

2011-11-20

Rio Grande do Sul Av. Bento Gonçalves 9500, Porto Alegre, POBox 15051 CEP 91501-970 Porto Alegre, RS, Brazil USAF, AFRL...Federal do Rio Grande do Sul P.O.Box 15051 91501-970 Porto Alegre, Rio Grande do Sul , Brasil URL: www.if.ufrgs.br/~marcia e-mail: marcia...nanoparticles in sílica xerogels by high pressure technique, Federal University of Rio Grande do Sul , December 2009. 2. Pamela Mantey dos

The novel compound Sul-121 inhibits airway inflammation and hyperresponsiveness in experimental models of chronic obstructive pulmonary disease

PubMed Central

Han, Bing; Poppinga, Wilfred J.; Zuo, Haoxiao; Zuidhof, Annet B.; Bos, I. Sophie T.; Smit, Marieke; Vogelaar, Pieter; Krenning, Guido; Henning, Robert H.; Maarsingh, Harm; Halayko, Andrew J.; van Vliet, Bernard; Stienstra, Stef; Graaf, Adrianus Cornelis van der; Meurs, Herman; Schmidt, Martina

2016-01-01

COPD is characterized by persistent airflow limitation, neutrophilia and oxidative stress from endogenous and exogenous insults. Current COPD therapy involving anticholinergics, β2-adrenoceptor agonists and/or corticosteroids, do not specifically target oxidative stress, nor do they reduce chronic pulmonary inflammation and disease progression in all patients. Here, we explore the effects of Sul-121, a novel compound with anti-oxidative capacity, on hyperresponsiveness (AHR) and inflammation in experimental models of COPD. Using a guinea pig model of lipopolysaccharide (LPS)-induced neutrophilia, we demonstrated that Sul-121 inhalation dose-dependently prevented LPS-induced airway neutrophilia (up to ~60%) and AHR (up to ~90%). Non-cartilaginous airways neutrophilia was inversely correlated with blood H2S, and LPS-induced attenuation of blood H2S (~60%) was prevented by Sul-121. Concomitantly, Sul-121 prevented LPS-induced production of the oxidative stress marker, malondialdehyde by ~80%. In immortalized human airway smooth muscle (ASM) cells, Sul-121 dose-dependently prevented cigarette smoke extract-induced IL-8 release parallel with inhibition of nuclear translocation of the NF-κB subunit, p65 (each ~90%). Sul-121 also diminished cellular reactive oxygen species production in ASM cells, and inhibited nuclear translocation of the anti-oxidative response regulator, Nrf2. Our data show that Sul-121 effectively inhibits airway inflammation and AHR in experimental COPD models, prospectively through inhibition of oxidative stress. PMID:27229886

Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells.

PubMed

Muriana, Francisco J G; Montserrat-de la Paz, Sergio; Lucas, Ricardo; Bermudez, Beatriz; Jaramillo, Sara; Morales, Juan C; Abia, Rocio; Lopez, Sergio

2017-08-01

Tyrosol (Tyr) is a phenolic compound found in virgin olive oil. After ingestion, Tyr undergoes extensive first pass intestinal/hepatic metabolism. However, knowledge about the biological effects of Tyr metabolites is scarce. We chemically synthesized Tyr glucuronate (Tyr-GLU) and sulphate (Tyr-SUL) metabolites and explored their properties against oxidative stress and inflammation in TNF-α-treated human umbilical vein endothelial cells (hECs). Tyr and Tyr-SUL prevented the rise of reactive oxygen species, the depletion of glutathione, and the down-regulation of glutathione peroxidase 1, glutamate-cysteine ligase catalytic subunit, and heme oxygenase-1 genes. Tyr-SUL and to a lower extent Tyr and Tyr-GLU prevented the phosphorylation of NF-κB signaling proteins. Tyr-GLU and Tyr-SUL also prevented the over-expression of adhesion molecules at gene, protein, and secretory levels, and the adhesion (Tyr-SUL > Tyr-GLU) of human monocytes to hECs. In vivo, Tyr, and most notably Tyr-SUL in a dose-dependent manner, ameliorated plantar and ear edemas in mice models of acute and chronic inflammation. This study demonstrates the antioxidant and/or anti-inflammatory properties of Tyr metabolites, with Tyr-SUL being the most effective.

The analysis of factors affecting the threshold on repeated 18F-FDG-PET/CT investigations measured by the PERCIST protocol in patients with esophageal carcinoma.

PubMed

Fencl, Pavel; Belohlavek, Otakar; Harustiak, Tomas; Zemanova, Milada

2012-11-01

When applying the PET Response Criteria in Solid Tumors protocol, a threshold value based on standardized uptake value corrected to lean body mass (SUL) in liver parenchyma, or in the blood pool, is used: to metabolically specify a measurable lesion; to calculate metabolic tumor volume (mTV) and its product total lesion glycolysis (TLG); and as a limit for response measurement. The problem with using changes in glucose metabolism as a marker for response to therapy is its reproducibility on test-retest examinations. Therefore, before the evaluation of tumor treatment response, we verified our diagnostic protocol for homogeneity using the PET Response Criteria in Solid Tumors quality parameters. In addition, we analyzed the effect of the time span between examinations on the average value of SUL (SUL MEAN) in liver parenchyma at three different points: first at baseline (BL), after the first course of chemotherapy (ChT1), and finally after finishing therapy (ChT3). We also analyzed the influence of SUL MEAN variation on mTV and TLG. Eighty-four patients with esophageal cancer were prospectively examined at BL using 2-deoxy-2-[18F]fluoro-D-glucose (18F-FDG)-PET/CT; 53 of 84 patients were examined after ChT1, 47 of 84 after ChT3, and 41 of 84 underwent all three examinations. Coefficient of variance (CV) and relative differences (RDw) were assessed for test-retest liver SUL values. The influence of liver SUL MEAN to mTV and TLG was modeled on BL examinations by artificial changes in liver SUL MEAN by ± 20%. No significant differences were found in test-retest liver SUL MEAN values. Comparing BL with ChT1, BL with ChT3, and ChT1 with ChT3, the CV of the liver SUL MEAN was 10.4, 10.7, and 10.3%; nevertheless, in 34.0, 38.3, and 36.6% of these examinations, respectively, the liver average SUL MEAN values exceeded the limit for inclusion in the study; that is, the difference was less than ± 0.3 U and ± 20%. The corresponding CV of blood background was 14.9, 16.5, and 17.2%. The artificial decrease of -20% in the liver SUL MEAN resulted in an increase of +43.6% in mTV and of +20.4% in TLG, whereas an increase of +20% in the liver SUL MEAN resulted in a decrease of -20.6% in mTV and -11.9% in TLG. SUL MEAN values in reference tissues (liver parenchyma or descending aorta) measured before chemotherapy did not differ significantly from those measured during chemotherapy. The CV of liver SUL MEAN was comparable to that seen in published data, but some patients had to be excluded from the study because of the individual variability of their mean liver SUL MEAN, which consequently hinders the clinical usage of mTV and TLG. Even in the standardized protocol, all potential sources of variability should be minimized.

Ferulic acid-4-O-sulfate rather than ferulic acid relaxes arteries and lowers blood pressure in mice.

PubMed

Van Rymenant, Evelien; Van Camp, John; Pauwels, Bart; Boydens, Charlotte; Vanden Daele, Laura; Beerens, Katrijn; Brouckaert, Peter; Smagghe, Guy; Kerimi, Asimina; Williamson, Gary; Grootaert, Charlotte; Van de Voorde, Johan

2017-06-01

Consumption of foods rich in ferulic acid (FA) such as wholegrain cereals, or FA precursors such as chlorogenic acids in coffee, is inversely correlated with risk of cardiovascular disease and type 2 diabetes. As a result of digestion and phase II metabolism in the gut and liver, FA is converted predominantly into ferulic acid-4-O-sulfate (FA-sul), an abundant plasma metabolite. Although FA-sul is the main metabolite, very little has been reported regarding its bioactivities. We have compared the ex vivo vasorelaxing effect of FA and FA-sul (10 -7 -3.10 -5 M) on isolated mouse arteries mounted in tissue myographs. FA-sul, but not FA, elicited a concentration-dependent vasorelaxation of saphenous and femoral arteries and aortae. The FA-sul-mediated vasorelaxation was blunted by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, a soluble guanylate cyclase (sGC) inhibitor. The role of sGC was confirmed in femoral arteries isolated from sGCα 1 (-/-) knockout mice. Furthermore, 4-aminopyridine, a specific inhibitor of voltage-dependent potassium channels, significantly decreased FA-sul-mediated effects. In anesthetized mice, intravenous injection of FA-sul decreased mean arterial pressure, whereas FA had no effect, confirming the results obtained ex vivo. FA-sul is probably one of the major metabolites accounting for the blood pressure-lowering effects associated with FA consumption. Copyright © 2017 Elsevier Inc. All rights reserved.

Novel sull binary vectors enable an inexpensive foliar selection method in Arabidopsis

USDA-ARS?s Scientific Manuscript database

Sulfonamide resistance is conferred by the sulI gene found on many Enterobacteriaceae R plasmids and Tn21 type transposons. The sulI gene encodes a sulfonamide insensitive dihydropteroate synthase enzyme required for folate biosynthesis. Transformation of tobacco, potato or Arabidopsis using sulI as...

Effect of UVA/LED/TiO2 photocatalysis treated sulfamethoxazole and trimethoprim containing wastewater on antibiotic resistance development in sequencing batch reactors.

PubMed

Cai, Qinqing; Hu, Jiangyong

2018-04-24

Controlling of antibiotics is the crucial step for preventing antibiotic resistance genes (ARGs) dissemination; UV photocatalysis has been identified as a promising pre-treatment technology for antibiotics removal. However, information about the effects of intermediates present in the treated antibiotics wastewater on the downstream biological treatment processes or ARGs development is very limited. In the present study, continuous UVA/LED/TiO 2 photocatalysis removed more than 90% of 100 ppb sulfamethoxazole (SMX)/trimethoprim (TMP), the treated wastewater was fed into SBR systems for over one year monitoring. Residual SMX/TMP (2-3 ppb) and intermediates present in the treated wastewater did not adversely affect SBR performance in terms of TOC and TN removal. SMX and TMP resistance genes (sulI, sulII, sulIII, dfrII, dfrV and dfr13) were also quantified in SBRs microbial consortia. Results suggested that continuous feeding of treated SMX/TMP containing wastewaters did not trigger any ARGs promotion during the one year operation. By stopping the input of 100 ppb SMX/TMP, abundance of sulII and dfrV genes were reduced by 83% and 100%, respectively. sulI gene was identified as the most persistence ARG, and controlling of 100 ppb SMX input did not achieve significant removal of sulI gene. A significant correlation between sulI gene and class 1 integrons was found at the level of p = 1.4E-10 (r = 0.94), and sulII gene positively correlated with the plasmid transfer efficiency (r = 2.442E-10, r = 0.87). Continuous input of 100 ppb SMX enhanced plasmid transfer efficiency in the SBR system, resulting in sulII gene abundance increasing more than 40 times. Copyright © 2018 Elsevier Ltd. All rights reserved.

Quantity of sucrose alters the tissue pattern and time course of insulin resistance in young rats.

PubMed

Pagliassotti, M J; Prach, P A

1995-09-01

To determine the effects of the amount of sucrose in the diet on insulin-stimulated glucose metabolism, euglycemic hyperinsulinemic clamps were performed on male Wistar rats after one of the following dietary treatments (n = 6-8/treatment): 1) high-starch diet (68% of total energy) for 8 wk (ST8), 16 wk (ST16), or 30 wk (ST30); 2) high-sucrose diet (68% of total energy) for 8 wk (SU8), 16 wk (SU16), or 30 wk (SU30); or 3) low-sucrose diet (18% of total energy) for 8 wk (SUL8), 16 wk (SUL16), or 30 wk (SUL30). Body weights were similar in starch- and sucrose-fed rats at 8 wk (502 +/- 9 g), 16 wk (563 +/- 10 g), and 30 wk (607 +/- 26 g). The glucose infusion rate (mumol.g-1.min-1) required to maintain similar glycemia during clamps was 73.1 +/- 8.8 in ST8, 29.7 +/- 4.9 in SU8 (P < 0.05 vs. ST8 and SUL8), and 76.4 +/- 8.2 in SUL8; 69.9 +/- 8.1 in ST16, 35.1 +/- 5.1 in SU16 (P < 0.05 vs. ST16 and SUL16), and 63.2 +/- 6.5 in SUL16; and 65.4 +/- 7.7 in ST30, 26.0 +/- 5.3 (P < 0.05 vs. ST30), and 36.3 +/- 6.0 in SUL30 (P < 0.05 vs. ST30). Impaired suppression of hepatic glucose production accounted for 43, 39, and 34% of the decrease in the glucose infusion rate in SU8 compared with ST8, SU16 compared with ST16, and SU30 compared with ST30, respectively, but 78% in SUL30 compared with ST30. These results suggest that both high- and low-sucrose diets can produce insulin resistance in young rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Abundance and persistence of antibiotic resistance genes in livestock farms: a comprehensive investigation in eastern China.

PubMed

Cheng, Weixiao; Chen, Hong; Su, Chao; Yan, Shuhai

2013-11-01

Increases of antibiotic resistance genes in the environment may pose a threat to public health. The purpose of this study was to investigate the abundance and diversity of tetracycline (tet) and sulfonamide (sul) resistance genes in eight livestock farms in Hangzhou, eastern China. Ten tet genes (tetA, tetB, tetC, tetG, tetL, tetM, tetO, tetQ, tetW, and tetX), two sul genes (sulI and sulII), and one genetic element associated with mobile antibiotic resistance genes [class 1 integron (intI1)] were quantified by real-time polymerase chain reaction. No significant difference was found in the abundance of the tet and sul genes in various scales of pig, chicken, and duck farms (P>0.05). The average abundance of ribosomal protection protein genes (tetQ, tetM, tetW, and tetO) in the manure and wastewater samples was higher than most of the efflux pump genes (tetA, tetB, tetC, and tetL) and enzymatic modification gene (tetX) (P<0.05), except for efflux pump gene tetG, which was abundant and showed no difference from tetM. Most ARGs had higher relative abundance in the wastewater lagoon than in manures even after treatment. Although the three ribosomal protection protein genes (tetQ, tetW, and tetO) had higher relative abundance, numbers were reduced during the complete wastewater treatment process in pig farms (P<0.05). The relative abundance of tetG, sulI, and sulII increased after the wastewater treatment and the removal of these three genes exhibited significant positive correlations with the intI1 gene (tetG: R(2)=0.60, P<0.05; sulI: R(2)=0.72, P<0.05; sulII: R(2)=0.62, P<0.05), suggesting that intI1 may be involved in their proliferation. As for tetM and sulII genes, a highly significant difference was found in manure samples between pig farms and duck farms (P<0.001). Phylogenetic analysis showed that tetM was more diverse in duck farms than in pig farms. Additionally, sulII sequence was conserved both in pig and duck farms. This is the first comprehensive study to detail the relative abundance of specific ARGs in animal manures and agricultural wastewater treatment systems, potentially providing knowledge for managing antibiotic resistance emanating from agricultural activities. © 2013.

Video and Field Observations of Wave Attenuation in a Muddy Surf Zone

DTIC Science & Technology

2011-01-01

Rio Grande do Sul ... Rio Grande , Rio Grande do Sul , Brazil. Tomazelli, L. 1993.0 regime dos ventos e a taxa de migracao das dunas eolicas costeiras do Rio Grande do Sul ...Institute of Oceanography. Federal University of Rio Grande . Av. Italia, km 8. CP. 474. Rio Grande . RS 96201-900. Brazil b College of Oceanic

Genotoxicity testing of sodium formononetin-3'-sulphonate (Sul-F) by assessing bacterial reverse mutation, chromosomal aberrations and micronucleus tests.

PubMed

Li, Chunmei; Gao, Yonglin; Wang, Yunzhi; Li, Guisheng; Fan, Xiaochen; Li, Yanshen; Guo, Chenghua; Tao, Jun

2017-06-01

As part of a safety evaluation, we evaluated the potential genotoxicity of sodium formononetin-3'-sulphonate (Sul-F) using bacterial reverse mutation assay, chromosomal aberrations detection, and mouse micronucleus test. In bacterial reverse mutation assay using five strains of Salmonella typhimurium (TA97, TA98, TA100, TA102 and TA1535), Sul-F (250, 500, 1000, 2000, 4000 μg/plate) did not increase the number of revertant colonies in any tester strain with or without S9 mix. In a chromosomal assay using Chinese hamster lung fibroblast (CHL) cells, there were no increases in either kind of aberration at any dose of Sul-F (400, 800, and 1600 μg/mL) treatment groups with or without S9 metabolic activation. In an in vivo bone marrow micronucleus test in ICR mice, Sul-F at up to 2000 mg/kg (intravenous injection) showed no significant increases in the incidence of micronucleated polychromatic erythrocytes, and the proportion of immature erythrocytes to total erythrocytes. The results demonstrated that Sul-F does not show mutagenic or genotoxic potential under these test conditions. Copyright © 2017. Published by Elsevier Inc.

Sulfamethoxazole and COD increase abundance of sulfonamide resistance genes and change bacterial community structures within sequencing batch reactors.

PubMed

Guo, Xueping; Pang, Weihai; Dou, Chunling; Yin, Daqiang

2017-05-01

The abundant microbial community in biological treatment processes in wastewater treatment plants (WWTPs) may potentially enhance the horizontal gene transfer of antibiotic resistance genes with the presence of antibiotics. A lab-scale sequencing batch reactor was designed to investigate response of sulfonamide resistance genes (sulI, sulII) and bacterial communities to various concentrations of sulfamethoxazole (SMX) and chemical oxygen demand (COD) of wastewater. The SMX concentrations (0.001 mg/L, 0.1 mg/L and 10 mg/L) decreased with treatment time and higher SMX level was more difficult to remove. The presence of SMX also significantly reduced the removal efficiency of ammonia nitrogen, affecting the normal function of WWTPs. All three concentrations of SMX raised both sulI and sulII genes with higher concentrations exhibiting greater increases. The abundance of sul genes was positive correlated with treatment time and followed the second-order reaction kinetic model. Interestingly, these two genes have rather similar activity. SulI and sulII gene abundance also performed similar response to COD. Simpson index and Shannon-Weiner index did not show changes in the microbial community diversity. However, the 16S rRNA gene cloning and sequencing results showed the bacterial community structures varied during different stages. The results demonstrated that influent antibiotics into WWTPs may facilitate selection of ARGs and affect the wastewater conventional treatment as well as the bacteria community structures. Copyright © 2017 Elsevier Ltd. All rights reserved.

Effect of temperature on sulfonamide antibiotics degradation, and on antibiotic resistance determinants and hosts in animal manures.

PubMed

Lin, Hui; Zhang, Jin; Chen, Hongjin; Wang, Jianmei; Sun, Wanchun; Zhang, Xin; Yang, Yuyi; Wang, Qiang; Ma, Junwei

2017-12-31

Animal manure is a main reservoir of antibiotic residues and antibiotic resistance. Here, the effect of temperature on sulfonamide antibiotics (SAs), sulfonamide-resistant (SR) genes/bacteria was investigated by aerobically incubating swine and chicken manures at different temperatures. In swine manure, the SAs concentration declined with increasing temperature, with a minimum at 60°C. In chicken manure, the greatest degradation of SAs was noted at 30°C. The reduction of relative abundance of antibiotic resistance genes (ARGs) and sul-positive hosts in swine manure was more pronounced during thermophilic than mesospheric incubation; neither temperature conditions effectively reduced these parameters in chicken manure. The relationship between the residual levels/distribution profiles of SAs, ARGs (sul1, sul2 and intI1), cultivable SR bacteria and sul-positive hosts was further established. The antibiotic residual profile, rather than antibiotic concentration, acted as an important factor in the prevalence of ARGs and sul-positive hosts in manure. Corynebacterium and Leucobacter from the phylum Actinobacteria tend to be main carriers of sul1 and intI1; the relative abundance of sul2 was significantly correlated with the relative abundance of cultivable SR bacteria. Overall, differences in resistant bacterial communities also constitute a dominant factor affecting ARG variation. This study contributes to management options for reducing the pollution of antibiotics and antibiotic resistance within manure. Copyright © 2017 Elsevier B.V. All rights reserved.

Direction control of anisotropy in the soft-magnetic underlayer for L10 Fe-Pt perpendicular media

NASA Astrophysics Data System (ADS)

Suzuki, Toshio

2005-05-01

Induced anisotropy of soft-magnetic underlayers (SUL) were pinned to radial and circumferential directions in double-layered perpendicular media, and effects of the directions on recording properties were studied for Fe-Pt media. A medium with the SUL of radial anisotropy showed a sharper cross-track profile than that of a medium with the SUL of circumferential anisotropy. Furthermore, signal-to-noise ratio (SNR) of the former was found to be 4dB higher than that of the latter at 500kfrpi. A SUL of radial anisotropy with an anisotropy-dispersion narrower could result in suppressing the fluctuation of write-field gradient and lead to further high SNR.

40 CFR 721.1550 - Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1).

Code of Federal Regulations, 2011 CFR

2011-07-01

...-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1). (a) Chemical substance and significant new... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1). 721.1550 Section 721.1550 Protection of Environment...

Differential paralog divergence modulates genome evolution across yeast species

PubMed Central

Lynch, Bryony; Huang, Mei; Alcantara, Erica; DeSevo, Christopher G.; Pai, Dave A.; Hoang, Margaret L.

2017-01-01

Evolutionary outcomes depend not only on the selective forces acting upon a species, but also on the genetic background. However, large timescales and uncertain historical selection pressures can make it difficult to discern such important background differences between species. Experimental evolution is one tool to compare evolutionary potential of known genotypes in a controlled environment. Here we utilized a highly reproducible evolutionary adaptation in Saccharomyces cerevisiae to investigate whether experimental evolution of other yeast species would select for similar adaptive mutations. We evolved populations of S. cerevisiae, S. paradoxus, S. mikatae, S. uvarum, and interspecific hybrids between S. uvarum and S. cerevisiae for ~200–500 generations in sulfate-limited continuous culture. Wild-type S. cerevisiae cultures invariably amplify the high affinity sulfate transporter gene, SUL1. However, while amplification of the SUL1 locus was detected in S. paradoxus and S. mikatae populations, S. uvarum cultures instead selected for amplification of the paralog, SUL2. We measured the relative fitness of strains bearing deletions and amplifications of both SUL genes from different species, confirming that, converse to S. cerevisiae, S. uvarum SUL2 contributes more to fitness in sulfate limitation than S. uvarum SUL1. By measuring the fitness and gene expression of chimeric promoter-ORF constructs, we were able to delineate the cause of this differential fitness effect primarily to the promoter of S. uvarum SUL1. Our data show evidence of differential sub-functionalization among the sulfate transporters across Saccharomyces species through recent changes in noncoding sequence. Furthermore, these results show a clear example of how such background differences due to paralog divergence can drive changes in genome evolution. PMID:28196070

The movable digital planetary from the Cruzeiro do Sul University as a distributing agent of astronomy

NASA Astrophysics Data System (ADS)

Voelzke, Marcos Rincon

2012-10-01

The Movable Digital Planetary from the Cruzeiro do Sul University has been working in order to publicize and to popularize Astronomy, in particular among students and teachers of Primary (EF) and Medium (EM) Education in municipal and state schools of the City of São Paulo, but also for the general public at large. The aim of this paper is to show and publicize the activities already undertaken by this planetary. In 2010, several presentations were recorded, such as: for the School Cruzeiro do Sul, in São Miguel Paulista, serving 161 children in the EF; Eighth Symposium on Education, Cruzeiro do Sul University, 75 students; NGO Educational Project Capuano, Anália Franco, 30 adults: Fair Student Guide in Shopping Center Norte, 455 people; NGO Association for Charitable Paulista, Burgo Paulista, 70 children; Workshop of Advanced Computing and Informatics, Cruzeiro do Sul University, 37 students; Day of Social Responsibility, Social Work in Don Bosco, Itaquera, 133 people! . In 2011 the presentations took place during the XIII Regional Meeting of Astronomy Education at Cruzeiro do Sul University, serving 112 teachers; College Cruzeiro do Sul, São Miguel Paulista, 356 children of the EF; College Brasilia from São Paulo, Anália Franco, 102 children in the EF and for the Scout Group Caramuru, São Paulo, 104 children. The applied methodology in all presentations consisted of the exhibition of two videos about Astronomy with a subsequent discussion about the presented issues. Previous surveys have shown a great interest in the majority of participants in wanting to learn more about the subject, which clearly explains the importance of education in non-formal places for the teaching of Astronomy

Incidence of the Recently Described Sulfonamide Resistance Gene sul3 among German Salmonella enterica Strains Isolated from Livestock and Food

PubMed Central

Guerra, Beatriz; Junker, Ernst; Helmuth, Reiner

2004-01-01

The sul3 gene recently described in Escherichia coli was found in 22 of 512 (4.3%) German Salmonella isolates from different regions and sources and of different serotypes, antimicrobial resistance phenotypes, and genomic groups. This is the first report on the prevalence of sul3 among Salmonella strains, and the findings support the strong potential of this determinant to spread within bacterial populations. PMID:15215132

Occurrence of sulfonamide-, tetracycline-, plasmid-mediated quinolone- and macrolide-resistance genes in livestock feedlots in Northern China.

PubMed

Mu, Quanhua; Li, Jin; Sun, Yingxue; Mao, Daqing; Wang, Qing; Luo, Yi

2015-05-01

Antibiotic resistance genes (ARGs) in livestock feedlots deserve attention because they are prone to transfer to human pathogens and thus pose threats to human health. In this study, the occurrence of 21 ARGs, including tetracycline (tet)-, sulfonamide (sul)-, plasmid-mediated quinolone (PMQR)- and macrolide-resistance (erm) genes were investigated in feces and adjacent soils from chicken, swine, and cattle feedlots in Northern China. PMQR and sul ARGs were the most prevalent and account for over 90.0 % of the total ARGs in fecal samples. Specifically, PMQR genes were the most prevalent, accounting for 59.6 % of the total ARGs, followed by sul ARGs (34.2 %). The percentage of tet ARGs was 3.4 %, and erm ARGs accounted for only 1.9 %. Prevalence of PMQR and sul ARGs was also found in swine and cattle feces. The overall trend of ARG concentrations in feces of different feeding animals was chicken > swine > beef cattle in the studied area. In soils, sul ARGs had the highest concentration and account for 71.1 to 80.2 % of the total ARGs, which is possibly due to the widely distributed molecular carriers (i.e., class one integrons), facilitating sul ARG propagation. Overall, this study provides integrated profiles of various types of ARGs in livestock feedlots and thus provides a reference for the management of antibiotic use in livestock farming.

Hospitalizations for primary care-sensitive conditions in Pelotas, Brazil: 1998 to 2012.

PubMed

Costa, Juvenal Soares Dias da; Teixeira, Ana Maria Ferreira Borges; Moraes, Mauricio; Strauch, Eliane Schneider; Silveira, Denise Silva da; Carret, Maria Laura Vidal; Fantinel, Everton

2017-01-01

To verify the hospitalization trend for primary care sensitive-conditions in Pelotas, Rio Grande do Sul, Brazil from 1998 to 2012. An ecological study compared hospitalizations rates of the city of Pelotas with the rest of state of Rio Grande do Sul. Analysis was conducted using direct standardization of rates, coefficients were stratified by sex and the Poisson regression was used. Hospitalizations for sensitive conditions decreased in Pelotas and Rio Grande do Sul. In Pelotas, a 63.8% decrease was detected in the period observed, and there was a 43.1% decrease in the state of Rio Grande do Sul. Poisson regression coefficients showed a decrease of 7% in Pelotas and of 4% in the rest of Rio Grande do Sul each year. During the study period, several changes were introduced in the Brazilian Unified Health System ("Sistema Único de Saúde") that may have influenced the results, including changes in administration, health funding, and a complete reworking of primary care through the creation of the Family Health Strategy program ("Estratégia Saúde da Família").

Emerging contaminants and nutrients synergistically affect the spread of class 1 integron-integrase (intI1) and sul1 genes within stable streambed bacterial communities.

PubMed

Subirats, Jèssica; Timoner, Xisca; Sànchez-Melsió, Alexandre; Balcázar, José Luis; Acuña, Vicenç; Sabater, Sergi; Borrego, Carles M

2018-07-01

Wastewater effluents increase the nutrient load of receiving streams while introducing a myriad of anthropogenic chemical pollutants that challenge the resident aquatic (micro)biota. Disentangling the effects of both kind of stressors and their potential interaction on the dissemination of antibiotic resistance genes in bacterial communities requires highly controlled manipulative experiments. In this work, we investigated the effects of a combined regime of nutrients (at low, medium and high concentrations) and a mixture of emerging contaminants (ciprofloxacin, erythromycin, sulfamethoxazole, diclofenac, and methylparaben) on the bacterial composition, abundance and antibiotic resistance profile of biofilms grown in artificial streams. In particular, we investigated the effect of this combined stress on genes encoding resistance to ciprofloxacin (qnrS), erythromycin (ermB), sulfamethoxazole (sul1 and sul2) as well as the class 1 integron-integrase gene (intI1). Only genes conferring resistance to sulfonamides (sul1 and sul2) and intI1 gene were detected in all treatments during the study period. Besides, bacterial communities exposed to emerging contaminants showed higher copy numbers of sul1 and intI1 genes than those not exposed, whereas nutrient amendments did not affect their abundance. However, bacterial communities exposed to both emerging contaminants and a high nutrient concentration (1, 25 and 1 mg L -1 of phosphate, nitrate and ammonium, respectively) showed the highest increase on the abundance of sul1 and intI1 genes thus suggesting a factors synergistic effect of both stressors. Since none of the treatments caused a significant change on the composition of bacterial communities, the enrichment of sul1 and intI1 genes within the community was caused by their dissemination under the combined pressure exerted by nutrients and emerging contaminants. To the best of our knowledge, this is the first study demonstrating the contribution of nutrients on the maintenance and spread of antibiotic resistance genes in streambed biofilms under controlled conditions. Our results also highlight that nutrients could enhance the effect of emerging contaminants on the dissemination of antibiotic resistance. Copyright © 2018 Elsevier Ltd. All rights reserved.

How fast was the Matuyama-Brunhes geomagnetic reversal? A new subcentennial record from the Sulmona Basin, central Italy

NASA Astrophysics Data System (ADS)

Sagnotti, Leonardo; Giaccio, Biagio; Liddicoat, Joseph C.; Nomade, Sebastien; Renne, Paul R.; Scardia, Giancarlo; Sprain, Courtney J.

2016-02-01

A recent study of the Matuyama-Brunhes (M-B) geomagnetic field reversal recorded in exposed lacustrine sediments from the Sulmona Basin (Italy) provided a continuous, high-resolution record indicating that the reversal of the field direction at the terminus of the M-B boundary (MBB) occurred in less than a century, about 786 ka ago. In the sediment, thin (4-6 cm) remagnetized horizons were recognized above two distinct tephra layers-SUL2-19 and SUL2-20-that occur ˜25 and ˜35 cm below the MBB, respectively. Also, a faint, millimetre-thick tephra (SUL2-18) occurs 2-3 cm above the MBB. With the aim of improving the temporal resolution of the previous Sulmona MBB record and understanding the possible influence of cryptotephra on the M-B record in the Sulmona Basin, we performed more detailed sampling and analyses of overlapping standard and smaller samples from a 50 cm-long block that spans the MBB. The new data indicate that (i) the MBB is even sharper than previously reported and occurs ˜2.5 cm below tephra SUL2-18, in agreement with the previous study; (ii) the MBB coincides with the rise of an intensity peak of the natural remanent magnetization (NRM) intensity, which extends across SUL2-18; (iii) except for a 2-cm-thick interval just above tephra SUL2-18, the rock magnetic parameters (k, ARM, Mr, Ms, Bc, Bcr) indicate exactly the same magnetic mineralogy throughout the sampled sequence. We conclude that either SUL2-18 resulted in the remagnetization of an interval of about 6 cm (i.e. during the NRM intensity peak spanning ˜260 ± 110 yr, according to the estimated local sedimentation rate), and thus the detailed MBB record is lost because it is overprinted, or the MBB is well recorded, occurred abruptly about 2.5 cm below SUL2-18 and lasted less than 13 ± 6 yr. Both hypotheses challenge our understanding of the geomagnetic field behaviour during a polarity transition and/or of the NRM acquisition process in the Sulmona lacustrine sediment.

Antimicrobial Resistance Genes in Pigeons from Public Parks in Costa Rica.

PubMed

Blanco-Peña, K; Esperón, F; Torres-Mejía, A M; de la Torre, A; de la Cruz, E; Jiménez-Soto, M

2017-11-01

Antimicrobial resistance is known to be an emerging problem, but the extent of the issue remains incomplete. The aim of this study was to determine the presence or absence of nine resistance genes (bla TEM , catI, mecA, qnrS, sulI, sulII, tet(A), tet(Q), vanA) in the faeces of 141 pigeons from four urban parks in Alajuela, Guadalupe, Tres Ríos and San José in Costa Rica. The genes were identified by real-time PCR directly from enema samples. About 30% of the samples were positive for genes catI and sulI; between 13% and 17% were positive for qnrS, sulII, tet(A) and tet(Q); and 4% were positive for bla TEM . The mecA and vanA genes were not detected. The average of antimicrobial resistance genes detected per pigeon was 2. Eight different patterns of resistance were identified, without differences in the sampling areas, being the most common pattern 2 (sulII positive samples). During rainy season, the genes more frequently found were sulI and tet(A). In conclusion, the urban inhabiting pigeons tested are currently carrying antimicrobial resistance genes, potentially acting as reservoirs of resistant bacteria and vectors to humans. To the authors' knowledge, this is the first study carried out on direct detection of resistance genes in the digestive metagenomes of pigeons. © 2017 The Authors. Zoonoses and Public Health Published by Blackwell Verlag GmbH.

Relationships between sulfachloropyridazine sodium, zinc, and sulfonamide resistance genes during the anaerobic digestion of swine manure.

PubMed

Zhang, Ranran; Gu, Jie; Wang, Xiaojuan; Qian, Xun; Duan, Manli; Sun, Wei; Zhang, Yajun; Li, Haichao; Li, Yang

2017-02-01

In this study, swine manure containing sulfachloropyridazine sodium (SCPS) and zinc was subjected to mesophilic (37°C) anaerobic digestion (AD). The absolute abundances (AAs) of antibiotic resistance genes (ARGs) were evaluated, as well as intI1 and intI2, and the degradation of SCPS according to variation in the amount of bio-available zinc (bio-Zn). In digester that only contained SCPS, the concentrations of SCPS were lower than that digesters both contain SCPS and Zn. Compared with the control digester, the addition of SCPS increased the AAs of sul1, sul3, drfA1, and drfA7 by 1.3-13.1 times. However, compared with the digester with SCPS but no added Zn, the AAs of sul3, drfA1, and drfA7 were decreased by 21.4-70.3% in the presence of SCPS and Zn, whereas sul1 and sul2 increased 1.3-10.7 times. There were significant positive correlations (P<0.05) between the concentrations of SCPS with several ARGs and bio-Zn. Copyright © 2016. Published by Elsevier Ltd.

Abundances and profiles of antibiotic resistance genes as well as co-occurrences with human bacterial pathogens in ship ballast tank sediments from a shipyard in Jiangsu Province, China.

PubMed

Lv, Baoyi; Cui, Yuxue; Tian, Wen; Li, Jing; Xie, Bing; Yin, Fang

2018-08-15

Ship ballasting operations may transfer harmful aquatic organisms across global ocean. This study aims to reveal the occurrences and abundances of antibiotic resistance genes (ARGs) and human bacterial pathogens (HBPs) in ballast tank sediments. Nine samples were collected and respectively analyzed by real-time quantitative PCR and high-throughput sequencing technologies. Ten ARGs (aadA1, blaCTX-M, blaTEM, ermB, mefA, strB, sul1, sul2, tetM, and tetQ) and the Class-I integron gene (intI1) were highly prevalent (10 5 -10 9 gene copies/g) in ballast tank sediments. The sul1 was the most abundant ARG with the concentration of 10 8 -10 9 copies/g and intI1 was much more abundant than the ARGs in ballast tank sediments. The strong positive correlations between intI1 and ARGs (blaCTX-M, sul1, sul2 and tetM) indicated the potential spread of ARGs via horizontal gene transfer. In ballast tank sediments, 44 bacterial species were identified as HBPs and accounted for 0.13-21.46% of the total bacterial population although the three indicator pathogenic microbes (Vibrio cholerae, Escherichia coli, and Enterococci) proposed by the International Maritime Organization were not detected. Pseudomonas pseudoalcaligenes, Enterococcus hirae, Shigella sonnei and Bacillus anthracis were the dominant pathogens in ballast tank sediments. Zn and P in sediments had positive effects on the ARGs. Network analysis results indicated that sul1 and sul2 genes existed in several bacterial pathogens. Ballast tank sediments could be regarded as a carrier for the migration of ARGs. It is important to manage ballast tank sediments reasonably in order to prevent the dissemination of ARGs and bacterial pathogens. Copyright © 2018 Elsevier Inc. All rights reserved.

Lavras do Sul: A New Equilibrated Ordinary L5 Chondrite from Rio Grande do Sul, Brazil

NASA Astrophysics Data System (ADS)

Zucolotto, M. E.; Antonello, L. L.; Varela, M. E.; Scorzelli, R. B.; Ludka, Isabel P.; Munayco, P.; dos Santos, E.

2012-03-01

The new Brazilian chondrite, Lavras do Sul, was found in 1985 at Lavras do Sul, Rio Grande do Sul State-Brazil (33°30'48″S; 53°54'65″W). It consists of a single mass weighing about 1 kg, covered by a black fusion crust with grayish interior. Four polished thin sections were prepared from a slice weighing 67 g on deposit at the Museu Nacional/UFRJ. It consists mostly of chondrules and chondrule fragments dispersed in a recrystallized matrix. Most chondrules are poorly defined and range in size from 300 to 2,000 μm, although some of them show distinct outlines, particularly when viewed under cross-polarized transmitted and reflected light. The texture of chondrules varies from non-porphyritic (e.g., barred-olivine, radial-pyroxene) to porphyritic ones (e.g., granular olivine as well as olivine-pyroxene). The meteorite contains mainly olivine (Fa24.9), low-Ca pyroxene (Fs22.6) and metal phases, with minor amounts of plagioclase, chromite and magnetite. Mössbauer Spectroscopy studies indicate that the metal phase is kamacite, tetrataenite and antitaenite. Veins of secondary iddingsite crosscut the thin section and some ferromagnesian silicates. The chemical composition indicates that Lavras do Sul is a member of the low iron L chondrite group. The poorly delineated chondritic texture with few well-defined chondrules, the occurrence of rare clinopyroxene and plagioclase (and maskelynite) with apparent diameters ranging from 5 to 123 μm led us to classify Lavras do Sul as an equilibrated petrologic type 5. The shock features of some minerals suggest a shock stage S3, and the presence of a small amount of secondary minerals such as iddingsite and goethite, a degree of weathering W1. The meteorite name was approved by the Nomenclature Committee (Nom Com) of the Meteoritical Society (Meteoritic Bulletin Nº99).

A continuous flow MFC-CW coupled with a biofilm electrode reactor to simultaneously attenuate sulfamethoxazole and its corresponding resistance genes.

PubMed

Li, Hua; Song, Hai-Liang; Yang, Xiao-Li; Zhang, Shuai; Yang, Yu-Li; Zhang, Li-Min; Xu, Han; Wang, Ya-Wen

2018-05-08

A continuous flow microbial fuel cell constructed wetland (MFC-CW) coupled with a biofilm electrode reactor (BER) system was constructed to remove sulfamethoxazole (SMX). The BER unit powered by the stacked MFC-CWs was used as a pretreatment unit, and effluent flowed into the MFC-CW for further degradation. The experimental results indicated that the removal rate of 2 or 4 mg/L SMX in a BER unit was nearly 90%, and the total removal rate in the coupled system was over 99%. As the hydraulic retention time (HRT) was reduced from 16 h to 4 h, the SMX removal rate in the BER decreased from 75% to 48%. However, the total removal rate in the coupled system was still over 97%. The maximum SMX removal rate in the MFC-CW, which accounted for 42%-55% of the total removal, was obtained in the anode layer. In addition, the relative abundances of sul genes detected in the systems were in the order of sulI > sulII > sulIII, and significant positive correlations of sul gene copy numbers versus SMX concentration and 16S rRNA gene copy numbers were observed. Furthermore, significant negative correlations were identified between sul genes, 16S rRNA gene copy numbers, and HRT. The abundances of the sul genes in the effluent of the MFC-CW were lower than the abundances observed in the BER effluent. High-throughput sequencing revealed that the microbial community diversity of the BER was affected by running time, power supply forms and HRT. Bio-electricity from the MFC-CW may reduce microbial community diversity and contribute to reduction of the antibiotic resistance gene (ARG) abundance in the BER. Taken together, the BER-MFC-CW coupled system is a potential tool to treat wastewater containing SMX and attenuate corresponding ARG abundance. Copyright © 2018 Elsevier B.V. All rights reserved.

Occurrences and Characterization of Antibiotic-Resistant Bacteria and Genetic Determinants of Hospital Wastewater in a Tropical Country.

PubMed

Le, Thai-Hoang; Ng, Charmaine; Chen, Hongjie; Yi, Xin Zhu; Koh, Tse Hsien; Barkham, Timothy Mark Sebastian; Zhou, Zhi; Gin, Karina Yew-Hoong

2016-12-01

Wastewater discharged from clinical isolation and general wards at two hospitals in Singapore was examined to determine the emerging trends of antibiotic resistance (AR). We quantified the concentrations of 12 antibiotic compounds by analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS), antibiotic-resistant bacteria (ARB), the class 1 integrase gene (intI1), and 16 antibiotic resistance genes (ARGs) that confer resistance to 10 different clinically relevant antibiotics. A subset of 119 antibiotic-resistant isolates were phylogenetically classified and tested for the presence of ARGs encoding resistance to β-lactam antibiotics (bla NDM , bla KPC , bla SHV , bla CTX-M ), amikacin [aac(6')-Ib], co-trimoxazole (sul1, sul2, dfrA), ciprofloxacin (qnrA, qnrB), and the intI1 gene. Among these resistant isolates, 80.7% were detected with intI1 and 66.4% were found to carry at least 1 of the tested ARGs. Among 3 sampled locations, the clinical isolation ward had the highest concentrations of ARB and the highest levels of ARGs linked to resistance to β-lactam (bla KPC ), co-trimoxazole (sul1, sul2, dfrA), amikacin [aac(6')-Ib], ciprofloxacin (qnrA), and intI1 We found strong positive correlations (P < 0.05) between concentrations of bacteria resistant to meropenem, ceftazidime, amikacin, co-trimoxazole, and ciprofloxacin and abundances of bla KPC , aac(6')-Ib, sul1, sul2, dfrA, qnrA, and intI1 genes. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Phytoseiid mites (Acari: Mesostigmata) from Araucaria Forest of the State of Rio Grande do Sul, Brazil, with new records and descriptions of four new species.

PubMed

Gonçalves, Dinarte; Cunha, Uemerson Silva Da; Bampi, Paula Maria; Moraes, Gilberto José De; Ferla, Noeli Juarez

2015-10-20

This paper reports on the Phytoseiidae from an Araucaria forest in the State of Rio Grande do Sul, describing four new species, namely Transeius kroeffis n. sp., Typhlodromalus araucariae n. sp., Typhlodromips pompeui n. sp. and Typhlodromips salvadorii n. sp.. Iphiseiodes moraesi Ferla & Silva, Neoseiulus tunus (DeLeon), Typhlodromips japi Lofego, Demite & Feres, Typhlodromips pallinii Gonçalves, Silva & Ferla, Typhloseiopsis dorsoreticulatus Lofego, Demite & Feres are reported for the first time from this type of habitat in Rio Grande do Sul state, Brazil.

Genetic progress in sunflower crop in Rio Grande do Sul State, Brazil.

PubMed

Follmann, D N; Cargnelutti Filho, A; Lorentz, L H; Boligon, A A; Caraffa, M; Wartha, C A

2017-04-13

The sunflower has adaptability to growing regions with different climatic and soil characteristics, showing drought tolerance and high-quality oil production. The State of Rio Grande do Sul is the third largest sunflower producer in Brazil, with research related to the sunflower breeding initiated after the decade of 1950. The aim of this study was to evaluate the genetic progress for grain yield, oil content, and oil yield of sunflower (Helianthus annuus L.) in the State of Rio Grande do Sul. Data of grain yield, oil content, and oil yield obtained from 58 sunflower cultivar yield trials in 19 municipalities in Rio Grande do Sul during the period from 2005 to 2014 were used. Genetic progress was studied according to the methodology proposed by Vencovsky and data from sunflower cultivar yield trials were used. Annual genetic progress of sunflower during the period of 10 years (2005-2014) was 132.46 kg⋅ha -1 ⋅year -1 for grain yield, -0.17%/year for oil content, and 48.11 kg⋅ha -1 ⋅year -1 for oil yield. The sunflower-breeding programs in the State of Rio Grande do Sul were efficient for the traits grain yield and oil yield and presented no efficiency for oil content.

Octadecylpropyl Sulfamide Reduces Neurodegeneration and Restores the Memory Deficits Induced by Hypoxia-Ischemia in Mice

PubMed Central

Kossatz, Elk; Silva-Peña, Daniel; Suárez, Juan; de Fonseca, Fernando R.; Maldonado, Rafael; Robledo, Patricia

2018-01-01

The PPAR-α agonist, oleoylethanolamide (OEA) has neuroprotective properties in stroke models. However, its rapid degradation represents a limitation for an effective therapeutic approach. In this study, we evaluated the effects of a stable OEA-modeled compound, octadecylpropyl sulfamide (SUL) on the cognitive, behavioral, cellular and molecular alterations associated with hypoxia-ischemia (HI) in mice. Mice subjected to HI were treated with the PPAR-α antagonist GW6471 (GW) (1 mg/kg) followed 15 min later by SUL (3 and 10 mg/kg). Behavioral, motor, and cognitive tests were carried out 24 h and 7 days after the HI. The levels of microglia, reactive astrocytes and neuronal nuclei were studied using immunofluorescence, and the expression of genes related to the N-acyl-ethanolamides/endocannabinoid signaling systems was determined by qRT-PCR at the end of the experimental sequence. HI induced brain damage in the ipsilateral hippocampus and cortex, which lead to severe memory impairments, and motor coordination deficits. Significant neuronal loss, increased microglia and reactive astrocytes, and compensatory changes in genes associated with the inflammation/immune and endocannabinoid systems were observed in these brain structures of lesioned mice. SUL reversed the memory and motor deficits, decreased the overexpression of microglia and astrocytes, and reduced neurodegeneration induced by HI. Cnr1 and Cnr2 gene expression was modulated by SUL in both sham and HI mice, while Pparα and Faah expression was regulated in HI mice. GW completely blocked the beneficial actions of SUL. These findings suggest that treatment with SUL reduces brain damage and the associated motor and memory deficits induced by HI probably by normalizing the changes in neuroinflammation/immune system mediators. PMID:29725299

Octadecylpropyl Sulfamide Reduces Neurodegeneration and Restores the Memory Deficits Induced by Hypoxia-Ischemia in Mice.

PubMed

Kossatz, Elk; Silva-Peña, Daniel; Suárez, Juan; de Fonseca, Fernando R; Maldonado, Rafael; Robledo, Patricia

2018-01-01

The PPAR-α agonist, oleoylethanolamide (OEA) has neuroprotective properties in stroke models. However, its rapid degradation represents a limitation for an effective therapeutic approach. In this study, we evaluated the effects of a stable OEA-modeled compound, octadecylpropyl sulfamide (SUL) on the cognitive, behavioral, cellular and molecular alterations associated with hypoxia-ischemia (HI) in mice. Mice subjected to HI were treated with the PPAR-α antagonist GW6471 (GW) (1 mg/kg) followed 15 min later by SUL (3 and 10 mg/kg). Behavioral, motor, and cognitive tests were carried out 24 h and 7 days after the HI. The levels of microglia, reactive astrocytes and neuronal nuclei were studied using immunofluorescence, and the expression of genes related to the N -acyl-ethanolamides/endocannabinoid signaling systems was determined by qRT-PCR at the end of the experimental sequence. HI induced brain damage in the ipsilateral hippocampus and cortex, which lead to severe memory impairments, and motor coordination deficits. Significant neuronal loss, increased microglia and reactive astrocytes, and compensatory changes in genes associated with the inflammation/immune and endocannabinoid systems were observed in these brain structures of lesioned mice. SUL reversed the memory and motor deficits, decreased the overexpression of microglia and astrocytes, and reduced neurodegeneration induced by HI. Cnr1 and Cnr2 gene expression was modulated by SUL in both sham and HI mice, while Ppar α and Faah expression was regulated in HI mice. GW completely blocked the beneficial actions of SUL. These findings suggest that treatment with SUL reduces brain damage and the associated motor and memory deficits induced by HI probably by normalizing the changes in neuroinflammation/immune system mediators.

Comparative in vitro activity of sulfametrole/trimethoprim and sulfamethoxazole/trimethoprim and other agents against multiresistant Gram-negative bacteria.

PubMed

Livermore, David M; Mushtaq, Shazad; Warner, Marina; Woodford, Neil

2014-04-01

Sulfamethoxazole/trimethoprim is standard therapy for infections caused by opportunist non-fermenters except Pseudomonas aeruginosa and Acinetobacter. Sulfametrol(e)/trimethoprim is an alternative to sulfamethoxazole/trimethoprim available in some EU countries, with possible pharmacological advantages. We compared their activities against (i) non-fermenters, (ii) multiresistant Enterobacteriaceae and (iii) reference strains with sul1 and sul2. Test isolates were recent submissions to the reference laboratory, or were Escherichia coli previously shown to have sul1 or sul2. Identification was by MALDI-ToF, by 16S rRNA gene sequencing or with API20NE strips. MICs were determined by CLSI agar dilution. The Stenotrophomonas maltophilia and Burkholderia series were enhanced by inclusion of 25% sulfamethoxazole/trimethoprim-resistant isolates; other series were not enhanced. MICs of sulfametrole/trimethoprim for non-fermenters tracked those of sulfamethoxazole/trimethoprim, being equal in 97/170 cases, 2-fold higher in 57/170 cases and 2-fold lower in 12/170 cases. Despite supplementing the Burkholderia and S. maltophilia collections with sulfamethoxazole/trimethoprim-resistant organisms, the antifolate combinations retained better activity against these and other non-fermenters than did piperacillin/tazobactam, moxifloxacin, ticarcillin/clavulanate, tigecycline, cefotaxime or imipenem. By contrast, few (5%-20%) of the extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacteriaceae were susceptible to the sulphonamides or their trimethoprim combinations, probably reflecting widespread co-carriage of sul1 and sul2, which both conferred resistance. Antifolate combinations remain the most active antimicrobials against less common non-fermenters, importantly including S. maltophilia and Burkholderia spp., but resistance is prevalent among ESBL- and carbapenemase-producing Enterobacteriaceae. Sulfametrole/trimethoprim had similar activity to sulfamethoxazole/trimethoprim against non-fermenters.

Sulphonated Formononetin Induces Angiogenesis through Vascular Endothelial Growth Factor/cAMP Response Element-Binding Protein/Early Growth Response 3/Vascular Cell Adhesion Molecule 1 and Wnt/β-Catenin Signaling Pathway.

PubMed

Dong, Zhaoju; Shi, Yanan; Zhao, Huijuan; Li, Ning; Ye, Liang; Zhang, Shuping; Zhu, Haibo

2018-01-01

Sodium formononetin-3'-sulphonate (Sul-F) is a derivative of the isoflavone formononetin. In this study, we investigated whether Sul-F can regulate angiogenesis and the potential mechanism in vitro. We examined the effects of Sul-F on cell proliferation, cell invasion, and tube formation in the human umbilical vein endothelial cell line (HUVEC). To better understand the mechanism involved, we investigated effects of the following compounds: cAMP response element-binding protein (CREB) inhibitor 2-naphthol-AS-E-phosphate (KG-501), early growth response 3 (Egr-3) siRNA, vascular endothelial growth factor (VEGF) antagonist soluble VEGF receptor 1 (sFlt-1), VEGF receptor 2 blocker SU-1498, Wnt5a antagonist WIF-1 recombinant protein (WIF-1), and inhibitor of Wnt/β-catenin recombinant Dickkopf-1 protein (DKK-1). HUVEC proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). A scratch adhesion test was used to assess cell invasion ability. Matrigel tube formation assay was performed to test capillary tube formation ability. Activation of the VEGF/CREB/Egr-3/Vascular cell adhesion molecule 1 (VCAM-1) pathway in HUVEC was tested by Western blot analysis. Our results suggest that Sul-F induced angiogenesis in vitro by enhancing cell proliferation, invasion, and tube formation. The increase in proliferation and tube formation by Sul-F was counteracted by DKK-1, WIF-1, SU1498, KG-501, sFlt-1, and Egr-3 siRNA. These results may suggest that Sul-F induces angiogenesis in vitro via a programed Wnt/β-catenin pathway and VEGF/CREB/Egr-3/VCAM-1 signaling axis. © 2017 S. Karger AG, Basel.

40 CFR 721.625 - Alkylated diarylamine, sul-furized (generic name).

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) TOXIC SUBSTANCES CONTROL ACT SIGNIFICANT NEW USES OF CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.625 Alkylated diarylamine, sul-furized (generic name). (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified generically...

Underreporting of gestational, congenital and acquired syphilis among indigenous peoples in Mato Grosso do Sul State, Brazil, 2011-2014.

PubMed

Tiago, Zuleica da Silva; Picoli, Renata Palópoli; Graeff, Samara Vilas-Boas; Cunha, Rivaldo Venâncio da; Arantes, Rui

2017-01-01

to describe the distribution, incidence, and underreporting of syphilis among indigenous peoples from Mato Grosso do Sul, Brazil. descriptive study performed with secondary data of the Information System for Notifiable Diseases (Sinan) and of the Special Indigenous Sanitary District of Mato Grosso do Sul (DSEI-MS), from 2011 to 2014; the data from both sources were compared to identify underreporting. the highest incidence rates of syphilis in pregnant women were observed in 2014 (41.1/1,000 live births) and of congenital syphilis, in 2013 (10.7/1,000 live births); the highest numbers of underreporting of cases were for syphilis in pregnant women on Sinan (45/79), of congenital syphilis at DSEI-MS (8/17) in 2014, and of acquired syphilis on Sinan in 2011 and 2013 (5/9 and 10/18, respectively). syphilis has a high incidence; underreporting hides the extent of the disease in indigenous peoples from Mato Grosso do Sul.

First record of Scobina poeciloides (Ashmead, 1895) (Hymenoptera: Argidae) for Brazil and update of geographical distribution of three species of Scobina Lepeletier & Serville, 1828 for the State of Rio Grande do Sul.

PubMed

Lemes, J R A; Köhler, A

2017-01-01

It is recorded for the first time in the state of Rio Grande do Sul the occurrence of Scobina melanocephala (Lepeletier, 1823), Scobina thoracica (Jorgensen, 1913) and Scobina poeciloides (Ashmead, 1895), being this last the first record for Brazil. Scobina melanopyga (Klug, 1834) and Scobina torquata (Konow, 1903) were also found in the study. The analyzed material was collected utilizing Malaise traps in tobacco (Nicotiana tabacum L.) fields and is deposited at the Entomological Collection of Santa Cruz do Sul.

PREVALENCE OF SULFONAMIDE AND FLORFENICOL RESISTANCE GENES IN ESCHERICHIA COLI ISOLATED FROM YAKS (BOS GRUNNIENS) AND HERDSMEN IN THE TIBETAN PASTURE.

PubMed

Zhang, Anyun; Yang, Yunfei; Wang, Hongning; Lei, Changwei; Xu, Changwen; Guan, Zhongbin; Liu, Bihui; Huang, Xi; Peng, Linyao

2015-07-01

To determine the antimicrobial susceptibility profiles and prevalence of resistance genes in Escherichia coli isolated from yaks (Bos grunniens) and herdsmen in nine plateau pastures in Tibet, we isolated 184 nonidentical strains of E. coli from yaks and herdsmen. Antimicrobial susceptibility testing of 15 antimicrobials was conducted and the prevalence of sulfonamide resistance genes (sul1, sul2, and sul3) and florfenicol resistance genes (floR, cfr, cmlA, fexA, pexA, and estDL136) was determined. Escherichia coli isolated from yaks had a high resistance rate to sulfamethoxazole (44%), sulphafurazole (40.4%), and florfenicol (11.4%). Escherichia coli isolated from herdsmen had a high resistance rate to sulfamethoxazole (57%) and sulphafurazole (51%). In addition, sul genes were present in 93% of sulfonamide-resistant isolates (84/90), and 17 floR genes and four cmlA genes were found in 19 florfenicol-resistant isolates. Even though florfenicol is prohibited from use in humans, three floR genes were detected in strains isolated from herdsmen. The three floR-positive isolates from herdsmen had pulsed-field gel electrophoresis patterns similar to isolates from yaks. In addition to documenting the sul and floR genes in E. coli isolated from yaks and herdsmen in the Tibetan pasture, we demonstrated the potential risk that antimicrobial-resistant E. coli could spread among herdsmen and yaks.

Genotype by environment interaction in Nelore cattle from five Brazilian states

PubMed Central

Diaz, Iara Del Pilar Solar; de Oliveira, Henrique Nunes; Bezerra, Luis Antônio Framartino; Lôbo, Raysildo Barbosa

2011-01-01

Records from 75,941 Nelore cattle were used to determine the importance of genotype by environment interaction (GEI) in five Brazilian states. (Co)variance components were estimated by single-trait analysis (with yearling weight, W450, considered to be the same trait in all states) and multiple-trait analysis (with the record from each state considered to be a different trait). The direct heritability estimates for yearling weight were 0.51, 0.39, 0.44, 0.37 and 0.41 in the states of Goiás, Mato Grosso, São Paulo, Mato Grosso do Sul and Minas Gerais, respectively. The across-state genetic correlation estimates between Goiás and Mato Grosso, Goiás and Minas Gerais, São Paulo and Minas Gerais, and Mato Grosso do Sul and Minas Gerais ranged from 0.67 to 0.75. These estimates indicate that GEIs are biologically important. No interactions were observed between Goiás and São Paulo, Goiás and Mato Grosso do Sul, Mato Grosso and São Paulo, Mato Grosso and Mato Grosso do Sul, Mato Grosso and Minas Gerais, or São Paulo and Mato Grosso do Sul (0.82 to 0.97). Comparison of single and multiple-trait analyses showed that selection based on the former was less efficient in the presence of GEI, with substantial losses (up to 10%) during selection. PMID:21931516

Reconnaissance for uranium in the coal of Sao Paulo, Santa Catarina, and Rio Grande do Sul, Brazil

USGS Publications Warehouse

Haynes, Donald D.; Pierson, Charles T.; White, Max G.

1958-01-01

Uranium-bearing coal and carbonaceous shale of the Rio Bonito formation of Pennsylvanian age have been found in the States of Sao Paulo, Santa Catarlna and Rio Grande do Sul, Brazil. The uranium oxide content of the samples collected in the State of Sao Paulo ranges from 0.001 percent to 0.082 percent. The samples collected in Santa Catarina averaged about 0.002 percent uranium oxide; those collected in Rio Grande do Sul, about 0.003 percent uranium oxide. Since the field and laboratory investigations are still in their initial stages, only raw data on the radioactivity and uranium content of Brazilian coals are given in this report.

Non-Linear Stability of Magnetically Focused Particle Beams

DTIC Science & Technology

2012-04-01

Grande do Sul, P.O.Box 15051,91501-970 Porto Alegre, RS, Brasil N/A 9. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR...Grande do Sul, Brasil www.if.ufrgs.br/~rizzato Grant: FA9550-09-1-0283 Final Performance Report April/2012 • Objectives Our main program involves the

Occurrence and prevalence of antibiotic resistance in landfill leachate.

PubMed

Wang, Yangqing; Tang, Wei; Qiao, Jing; Song, Liyan

2015-08-01

Antibiotic resistance (AR) is extensively present in various environments, posing emerging threat to public and environmental health. Landfill receives unused and unwanted antibiotics through household waste and AR within waste (e.g., activated sludge and illegal clinical waste) and is supposed to serve as an important AR reservoir. In this study, we used culture-dependent methods and quantitative molecular techniques to detect and quantify antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in 12 landfill leachate samples from six geographic different landfills, China. Five tested ARGs (tetO, tetW, bla(TEM), sulI, and sulII) and seven kinds of antibiotic-resistant heterotrophic ARB were extensively detected in all samples, demonstrating their occurrence in landfill. The detected high ratio (10(-2) to 10(-5)) of ARGs to 16S ribosomal RNA (rRNA) gene copies implied that ARGs are prevalent in landfill. Correlation analysis showed that ARGs (tetO, tetW, sulI, and sulII) significantly correlated to ambient bacterial 16S rRNA gene copies, suggesting that the abundance of bacteria in landfill leachate may play an important role in the horizontal spread of ARGs.

New Form Discovery for the Analgesics Flurbiprofen and Sulindac Facilitated by Polymer-Induced Heteronucleation

PubMed Central

GRZESIAK, ADAM L.; MATZGER, ADAM J.

2008-01-01

The selection and discovery of new crystalline forms is a longstanding issue in solid-state chemistry of critical importance because of the effect molecular packing arrangement exerts on materials properties. Polymer-induced heteronucleation has recently been developed as a powerful approach to discover and control the production of crystal modifications based on the insoluble polymer heteronucleant added to the crystallization solution. The selective nucleation and discovery of new crystal forms of the well-studied pharmaceuticals flurbiprofen (FBP) and sulindac (SUL) has been achieved utilizing this approach. For the first time, FBP form III was produced in bulk quantities and its crystal structure was also determined. Furthermore, a novel 3:2 FBP:H2O phase was discovered that nucleates selectively from only a few polymers. Crystallization of SUL in the presence of insoluble polymers facilitated the growth of form I single crystals suitable for structure determination. Additionally, a new SUL polymorph (form IV) was discovered by this method. The crystal forms of FBP and SUL are characterized by Raman and FTIR spectroscopies, X-ray diffraction, and differential scanning calorimetry. PMID:17567888

Comparative study on mortality due to cardiovascular diseases in São Caetano do Sul, São Paulo, Brazil, between 1980 and 2010.

PubMed

Luz, Fernanda Eugenio da; Santos, Brigitte Rieckmann Martins Dos; Sabino, Wilson

2017-01-01

Analysis of the mortality due to cardiovascular diseases (CVD) can provide subsidies for preventive and control measures. The goal of this article is to compare CVD mortality rates in São Caetano do Sul, the state of São Paulo and the country as a whole. Standardized mortality and mortality due to CVD were calculated for the 1980-2010 period. We found a significant reduction in cardiovascular mortality in all three study units during this period, with the largest reduction in CVD in São Caetano do Sul. The largest mortality rate was found in the state of São Paulo. In adults 30 to 59, the CVD mortality rate in São Caetano do Sul was three times as high in men as in women, yet among adults 60 and older, CVD mortality was higher in women than in men. The lower rate is the result of implementing different healthcare policies. However, specific interventions are required that focus on changes in lifestyle, especially among adult men and the elderly.

Spiramycin/cotrimoxazole versus pyrimethamine/sulfonamide and spiramycin alone for the treatment of toxoplasmosis in pregnancy.

PubMed

Valentini, P; Buonsenso, D; Barone, G; Serranti, D; Calzedda, R; Ceccarelli, M; Speziale, D; Ricci, R; Masini, L

2015-02-01

To compare the effectiviness of spiramycin/cotrimoxazole (Sp/C) versus pyrimethamine/sulfonamide (Pyr/Sul) and spiramycin alone (Spy) on mother-to-child transmission of toxoplasmosis infection in pregnancy. Retrospective study of pregnant women evaluated for suspected toxoplasmosis between 1992 and 2011. A total of 120 mothers and their 123 newborns were included. Prenatal treatment consisted of spiramycin in 43 mothers (35%), spiramycin/cotrimoxazole in 70 (56.9%) and pyrimethamine/sulfonamide in 10 (8.1%). A trend toward reduction in toxoplasmosis transmission was found when Sp/C was compared with Pyr/Sul and particularly with Spy alone (P=0.014). In particular, Spy increased the risk of congenital infection when compared with Sp/C (odds ratio (OR) 4.368; 95% CI: 1.253 to 15.219), but there was no significant reduction when Sp/C was compared with Pyr/Sul (OR 1.83; 95% CI: 0.184 to 18.274). The treatment based on Sp/C has significant efficacy in reducing maternal-fetal transmission of Toxoplasma gondii when compared with Pyr/Sul and particularly to Spy. Randomized controlled trials would be required.

Cardioprotective effect of sulphonated formononetin on acute myocardial infarction in rats.

PubMed

Zhang, Shumin; Tang, Xuexi; Tian, Jingwei; Li, Chunmei; Zhang, Guanbo; Jiang, Wanglin; Zhang, Zunting

2011-06-01

This study was designed to investigate the therapeutic effect of sodium formononetin-3'-sulphonate (Sul-F), a water-soluble derivate of formononetin, on acute myocardial infarction in rats. The results showed that treatment with Sul-F significantly prevented the elevation of ST-segment level, decreased the contents of creatine kinase-MB, lactate dehydrogenase, alanine aminotransferase and cardiac troponin T in serum and reduced the myocardium necrosis scores. The number of apoptosis cardiocytes is well accordance with the up-regulated expression of Bcl-2 and the down-regulated expression of Bax. Meanwhile, Sul-F significantly increased the cardiac mitochondrial ATP content, improved ATP synthase activity, decreased thiobarbituric acid-reactive substances content and attenuated the decrease in superoxide dismutase and glutathione peroxidase activities. These findings indicate that Sul-F has a protective potential against myocardial infarction injury. A possible mechanism for the protective effect is the elevated expression of endogenous antioxidant defence enzymes degraded lipid peroxidation products and improved energy metholism of cardiac mitochondrial, thus attenuating cardiocyte apoptosis. © 2011 The Authors. Basic & Clinical Pharmacology & Toxicology © 2011 Nordic Pharmacological Society.

Characterization of Integrons and Sulfonamide Resistance Genes among Bacteria from Drinking Water Distribution Systems in Southwestern Nigeria.

PubMed

Adesoji, Ayodele T; Ogunjobi, Adeniyi A; Olatoye, Isaac O

2017-01-01

The emergence of antibiotic resistance among pathogenic bacteria in clinical and environmental settings is a global problem. Many antibiotic resistance genes are located on mobile genetic elements such as plasmids and integrons, enabling their transfer among a variety of bacterial species. Water distribution systems may be reservoirs for the spread of antibiotic resistance. Bacteria isolated from raw, treated, and municipal tap water samples from selected water distribution systems in south-western Nigeria were investigated using the point inoculation method with seeded antibiotics, PCR amplification, and sequencing for the determination of bacterial resistance profiles and class 1/2 integrase genes and gene cassettes, respectively. sul1,sul2, and sul3 were detected in 21.6, 27.8, and 0% of the isolates, respectively (n = 162). Class 1 and class 2 integrons were detected in 21.42 and 3.6% of the isolates, respectively (n = 168). Genes encoding resistance to aminoglycosides (aadA2, aadA1, and aadB), trimethoprim (dfrA15, dfr7, and dfrA1), and sulfonamide (sul1) were detected among bacteria with class 1 integrons, while genes that encodes resistance to strepthothricin (sat2) and trimethoprim (dfrA15) were detected among bacteria with class 2 integrons. Bacteria from these water samples are a potential reservoir of multidrug-resistant traits including sul genes and mobile resistance elements, i.e. the integrase gene. © 2016 S. Karger AG, Basel.

Structure analysis of a heteropolysaccharide from Taraxacum mongolicum Hand.-Mazz. and anticomplementary activity of its sulfated derivatives.

PubMed

Chen, MiaoMiao; Wu, Jianjun; Shi, Songshan; Chen, Yonglin; Wang, Huijun; Fan, Hongwei; Wang, Shunchun

2016-11-05

A homogenous water-soluble polysaccharide, DPSW-A, with a deduced chemical structure was extracted from the herb Taraxacum mongolicum Hand.-Mazz. Moreover, 80.813-kDa DPSW-A is composed of three types of monosaccharide, namely rhamnose, arabinose, and galactose, at a molar ratio of 1.0:10.7:11.9. The main chain of DPSW-A contains Terminal-Galp, 1,3-Galp, 1,6-Galp, 1,3,6-Galp, and 1,2,4-Rhap; the branched chain contains Terminal-Araf, 1,5-Araf, and 1,3,5-Araf. The sulfated derivatives prepared from DPSW-A showed inhibitory effects on complement activation through the classical pathway (CH50: Sul-DPSW-A, 3.94±0.43μg/mL; heparin, 104.40±3.82μg/mL) and alternative pathway (AP50: Sul-DPSW-A, 42.76±0.46μg/mL; heparin, 43.42±0.22μg/mL). Mechanism studies indicated that Sul-DPSW-A inhibited complement activation by blocking C1q, C1r, C1s, and C9, but not C2, C3, C4, and C5. In addition, Sul-DPSW-A displayed limited anticoagulant effects. These results suggest that Sul-DPSW-A prepared from DPSW-A is valuable for treating diseases caused by excessive complement system activation. Copyright © 2016 Elsevier Ltd. All rights reserved.

Genetic redundancy and persistence of plasmid-mediated trimethoprim/sulfamethoxazole resistant effluent and stream water Escherichia coli.

PubMed

Suhartono, Suhartono; Savin, Mary; Gbur, Edward E

2016-10-15

Antibiotic resistant bacteria may persist in effluent receiving surface water in the presence of low (sub-inhibitory) antibiotic concentrations if the bacteria possess multiple genes encoding resistance to the same antibiotic. This redundancy of antibiotic resistance genes may occur in plasmids harboring conjugation and mobilization (mob) and integrase (intI) genes. Plasmids extracted from 76 sulfamethoxazole-trimethoprim resistant Escherichia coli originally isolated from effluent and an effluent-receiving stream were used as DNA template to identify sulfamethoxazole (sul) and trimethoprim (dfr) resistances genes plus detect the presence of intI and mob genes using PCR. Sulfamethoxazole and trimethoprim resistance was plasmid-mediated with three sul (sul1, sul2 and sul3 genes) and four dfr genes (dfrA12, dfrA8, dfrA17, and dfrA1 gene) the most prevalently detected. Approximately half of the plasmids carried class 1 and/or 2 integron and, although unrelated, half were also transmissible. Sampling site in relationship to effluent input significantly affected the number of intI and mob but not the number of sul and dfr genes. In the presence of low (sub-inhibitory) sulfamethoxazole concentration, isolates persisted regardless of integron and mobilization gene designation, whereas in the presence of trimethoprim, the presence of both integron and mobilization genes made isolates less persistent than in the absence of both or the presence of a gene from either group individually. Regardless, isolates persisted in large concentrations throughout the experiment. Treated effluent containing antibiotic resistant bacteria may be an important source of integrase and mobilization genes into the stream environment. Sulfamethoxazole-trimethoprim resistant bacteria may have a high degree of genetic redundancy and diversity carrying resistance to each antibiotic, although the role of integrase and mobilization genes towards persistence is unclear. Copyright © 2016 Elsevier Ltd. All rights reserved.

A New species and records of Gripopterygidae (Plecoptera) from Rio Grande do Sul State, Southern Brazil.

PubMed

Novaes, Marcos Carneiro; Da Conceição Bispo, Pitágoras

2016-10-17

Specimens of Gripopterygidae (Plecoptera) from Rio Grande do Sul State, southern Brazil were studied. A new species, Tupiperla sepeensis n. sp. is described. Tupiperla misionera Froehlich 2002 is a new record for Brazil and Gripopteryx reticulata Brauer 1866 and Tupiperla tessellata Brauer 1866 are new records for southern Brazil.

Why Children Fail in First Grade in Rio Grande do Sul: Implications for Policy and Research.

ERIC Educational Resources Information Center

Wolff, Laurence

This study, exploring why first grade children from Rio Grande do Sul, Brazil, fail in school, utilized computerized techniques of statistical analysis to measure the relationships of various school and family characteristics with student achievement. Four types of schools--urban state, rural state, municipal, and private--were used to test the…

[History of genetics in Brazil: a view from the Museu da Genética at the Universidade Federal do Rio Grande do Sul].

PubMed

Souza, Vanderlei Sebastiao de; Dornelles, Rodrigo Ciconet; Coimbra Junior, Carlos E A; Santos, Ricardo Ventura

2013-06-01

This work addresses the context of the creation, as well as the structure and contents, of the Museum of Genetics (Museu da Genética), created in 2011 and located in the Department of Genetics of the Federal University of Rio Grande do Sul (Universidade Federal do Rio Grande do Sul), in Porto Alegre, Brazil. The materials available at the Museum of Genetics are a rich resource for research on the history of genetics in Brazil (and especially the genetics of human populations) beginning with the second half of the twentieth century. Despite the prominence of the field of genetics in Brazil, little research has been done on this topic.

Palaeomagnetic directions of the volcanic rocks from Gramado Xavier, Rio Grande do Sul State, South Brazil: implications for time duration of the volcanic activity.

NASA Astrophysics Data System (ADS)

Raposo, M. I. B.; Canon-Tapia, E.; Guimarães, L. F.; Janasi, V. A.

2015-12-01

The magmatism in the LIP Paraná-Etendeka comprises basic and acid rocks. On the Paraná side, these rocks are basalt tholeiitic with high (>2%) and low TiO2 content, and dacites, rhyodacites, rhyolites and quartz latites forming the acid types Chapecó and Palmas. The volcanic acid Palmas are found in the South part of Brazil, and based on TiO2 and P2O5 contents are subdivided into Caxias do Sul, Santa Maria, Anita Garibaldi, Jacuí, Clevelândia and Barros Cassal units. In the studied region, the first stratigraphic sequence is low TiO2 basalt followed by Caxias do Sul, Barros Cassal and Santa Maria on top. We sampled all these units in the Gramado Xavier (Rio Grande do Sul State, South Brazil) region. To determine the mean magnetization directions of each site, samples were demagnetized by both thermal and AF techniques. The results show that the basalt flows recorded both normal and reverse polarities of the geomagnetic field. All sites from Caxias do Sul registered an anomalous direction suggesting an excursion of the geomagnetic field. Sites from Barros Cassal present both normal and reverse polarities. All sites from Santa Maria unit show a reverse polarity of the geomagnetic field. The normal and reverse polarities recorded in the different units are similar indicating contemporaneity of the magmatic source. Due to the existence of only one reversal event, a short duration of volcanism is suspected.

Bacterial community structure and abundances of antibiotic resistance genes in heavy metals contaminated agricultural soil.

PubMed

Zhang, Fengli; Zhao, Xiaoxue; Li, Qingbo; Liu, Jia; Ding, Jizhe; Wu, Huiying; Zhao, Zongsheng; Ba, Yue; Cheng, Xuemin; Cui, Liuxin; Li, Hongping; Zhu, Jingyuan

2018-04-01

Soil contamination with heavy metals is a worldwide problem especially in China. The interrelation of soil bacterial community structure, antibiotic resistance genes, and heavy metal contamination in soil is still unclear. Here, seven agricultural areas (G1-G7) with heavy metal contamination were sampled with different distances (741 to 2556 m) to the factory. Denaturing gradient gel electrophoresis (DGGE) and Shannon index were used to analyze bacterial community diversity. Real-time fluorescence quantitative PCR was used to detect the relative abundance of ARGs sul1, sul2, tetA, tetM, tetW, one mobile genetic elements (MGE) inti1. Results showed that all samples were polluted by Cadmium (Cd), and some of them were polluted by lead (Pb), mercury (Hg), arsenic (As), copper (Cu), and zinc (Zn). DGGE showed that the most abundant bacterial species were found in G7 with the lightest heavy metal contamination. The results of the principal component analysis and clustering analysis both showed that G7 could not be classified with other samples. The relative abundance of sul1 was correlated with Cu, Zn concentration. Gene sul2 are positively related with total phosphorus, and tetM was associated with organic matter. Total gene abundances and relative abundance of inti1 both correlated with organic matter. Redundancy analysis showed that Zn and sul2 were significantly related with bacterial community structure. Together, our results indicate a complex linkage between soil heavy metal concentration, bacterial community composition, and some global disseminated ARG abundance.

Field data observed during the geological excursion in the west-central region of the Sul-Riogrande Shield

NASA Technical Reports Server (NTRS)

Parada, N. D. J. (Principal Investigator); Ohara, T.

1984-01-01

Outcrops are studied in the Copper Project test area of the Rio Grande do Sul State of Brazil. The accuracy of LANDSAT-MSS data is checked against field data. A preliminary geological map is included on a scale of 1:500,000 that describes 820 outcrop over an area of 1,700 kilometers.

First record of Scybalocanthon nigriceps (Harold, 1868) (Coleoptera: Scarabaeidae: Scarabaeinae) in Rio Grande do Sul state, southern Brazil.

PubMed

Ferreira, Sheila C; Mare, Rocco A DI; Silva, Pedro G DA

2017-01-01

The dung beetle, Scybalocanthon nigriceps (Harold, 1868), is recorded in Rio Grande do Sul state, Brazil, for the first time, at the Moreno Fortes Biological Reserve, municipality of Dois Irmãos das Missões, northwest region of the state, expanding the area of occurrence and distribution of this species in the country.

Epidemiological and biological aspects on Ornithodoros brasiliensis (mouro tick), an argasidae tick only found on the highlands region of Rio Grande do Sul state, southern Brazil

USDA-ARS?s Scientific Manuscript database

The soft tick Ornithodoros brasiliensis (Acari: Argasidae) is present in farms along the highlands of Rio Grande do Sul state in southern Brazil. Reports of human parasitism by O. brasiliensis drew the attention of local health authorities. A preliminary epidemiological survey was conducted to ident...

Sulphonamide and Trimethoprim Resistance Genes Persist in Sediments at Baltic Sea Aquaculture Farms but Are Not Detected in the Surrounding Environment

PubMed Central

Muziasari, Windi Indra; Managaki, Satoshi; Pärnänen, Katariina; Karkman, Antti; Lyra, Christina; Tamminen, Manu; Suzuki, Satoru; Virta, Marko

2014-01-01

Persistence and dispersal of antibiotic resistance genes (ARGs) are important factors for assessing ARG risk in aquaculture environments. Here, we quantitatively detected ARGs for sulphonamides (sul1 and sul2) and trimethoprim (dfrA1) and an integrase gene for a class 1 integron (intI1) at aquaculture facilities in the northern Baltic Sea, Finland. The ARGs persisted in sediments below fish farms at very low antibiotic concentrations during the 6-year observation period from 2006 to 2012. Although the ARGs persisted in the farm sediments, they were less prevalent in the surrounding sediments. The copy numbers between the sul1 and intI1 genes were significantly correlated suggesting that class 1 integrons may play a role in the prevalence of sul1 in the farm sediments through horizontal gene transfer. In conclusion, the presence of ARGs may limit the effectiveness of antibiotics in treating fish illnesses, thereby causing a potential risk to the aquaculture industry. However, the restricted presence of ARGs at the farms is unlikely to cause serious effects in the northern Baltic Sea sediment environments around the farms. PMID:24651770

First 'Rauisuchian' archosaur (Pseudosuchia, Loricata) for the Middle Triassic Santacruzodon assemblage zone (Santa Maria Supersequence), Rio Grande do Sul State, Brazil.

PubMed

Lacerda, Marcel B; Schultz, Cesar L; Bertoni-Machado, Cristina

2015-01-01

The 'Rauisuchia' are a group of Triassic pseudosuchian archosaurs that displayed a near worldwide distribution. In Brazil, their fossils are found only in the Santa Maria Formation (Paraná Basin) of the Rio Grande do Sul State, specifically in the Middle Triassic Dinodontosaurus assemblage zone (AZ) and the Late Triassic Hyperodapedon AZ (Rauisuchus tiradentes). Between these two cenozones is the Santacruzodon AZ (Middle Triassic), whose record was, until now, restricted to non-mammalian cynodonts and the proterochampsian Chanaresuchus bonapartei. Here we present the first occurrence of a rauisuchian archosaur for this cenozone, from the Schoenstatt outcrop, located near the city of Santa Cruz do Sul and propose a new species, based on biostratigraphical evidence and a comparative osteological analysis.

First 'Rauisuchian' archosaur (Pseudosuchia, Loricata) for the Middle Triassic Santacruzodon Assemblage Zone (Santa Maria Supersequence), Rio Grande do Sul State, Brazil

PubMed Central

Lacerda, Marcel B.; Schultz, Cesar L.; Bertoni-Machado, Cristina

2015-01-01

The ‘Rauisuchia’ are a group of Triassic pseudosuchian archosaurs that displayed a near worldwide distribution. In Brazil, their fossils are found only in the Santa Maria Formation (Paraná Basin) of the Rio Grande do Sul State, specifically in the Middle Triassic Dinodontosaurus assemblage zone (AZ) and the Late Triassic Hyperodapedon AZ (Rauisuchus tiradentes). Between these two cenozones is the Santacruzodon AZ (Middle Triassic), whose record was, until now, restricted to non-mammalian cynodonts and the proterochampsian Chanaresuchus bonapartei. Here we present the first occurrence of a rauisuchian archosaur for this cenozone, from the Schoenstatt outcrop, located near the city of Santa Cruz do Sul and propose a new species, based on biostratigraphical evidence and a comparative osteological analysis. PMID:25714091

[Positivism and medical science in Rio Grande do Sul: the Faculdade de Medicina de Porto Alegre].

PubMed

Weber, B T

1998-01-01

The article analyzes conflicts and interests at one of Rio Grande de Sul's main centers for medical science, the Faculdade de Medicina de Porto Alegre. It explores the meaning and impact of the emergence of this specific, exclusive field of knowledge in a state where positivist principles of professional freedom were adopted by successive administrations during the early period of the Republic. Physicians there launched an entrenched war to uphold the principles of science over faith and politics, challenging the positivism of the party which held power in Rio Grande do Sul throughout the years. This perspective grew and developed in a climate of conflict and doubts among physicians, within a political context that differed from the rest of Brazil.

Prevalence of antibiotic resistance genes in bacteriophage DNA fraction from Funan River water in Sichuan, China.

PubMed

Yang, Yanxian; Shi, Wenjin; Lu, Shao-Yeh; Liu, Jinxin; Liang, Huihui; Yang, Yifan; Duan, Guowei; Li, Yunxia; Wang, Hongning; Zhang, Anyun

2018-06-01

To better understand the role that bacteriophages play in antibiotic resistance genes (ARGs) dissemination in the aquatic environment, 36 water samples were collected from the Funan River in Sichuan, China. The occurrence of 15 clinically relevant ARGs and one class 1 integron gene int1 in phage-particle DNA were evaluated by PCR. The abundance of ARGs (bla CTX-M , sul1, and aac-(6')-1b-cr) was determined by quantitative PCR (qPCR). High prevalence of the int1 gene (66.7%) was found in the phage-particle DNA of tested samples, followed by sul1 (41.7%), sul2 (33.3%), bla CTX-M (33.3%), aac-(6')-lb-cr (25%), aph(3')-IIIa (16.7%), and ermF (8.3%). The qPCR data showed higher gene copy (GC) numbers in samples collected near a hospital (site 7) and a wastewater treatment plant (WWTP) (site 10) (P < .05). Particularly the absolute abundance of aac-(6')-lb-cr gene was significantly higher than the bla CTX-M and sul1 genes with the gene copy (GC) numbers of 5.73 log 10  copy/mL for site 7 and 4.99 log 10  copy/mL for site 10. To our best knowledge, this is the first study to report the presence of sul2, aac-(6')-lb-cr, ermF and aph(3')-IIIa genes in bacteriophage DNA derived from aquatic environments. Our findings highlight the potential of ARGs to be transmitted via bacteriophages in the aquatic environment. Copyright © 2018 Elsevier B.V. All rights reserved.

Increased Abundance and Transferability of Resistance Genes after Field Application of Manure from Sulfadiazine-Treated Pigs

PubMed Central

Jechalke, Sven; Kopmann, Christoph; Rosendahl, Ingrid; Groeneweg, Joost; Weichelt, Viola; Krögerrecklenfort, Ellen; Brandes, Nikola; Nordwig, Mathias; Ding, Guo-Chun; Siemens, Jan; Heuer, Holger

2013-01-01

Spreading manure containing antibiotics in agriculture is assumed to stimulate the dissemination of antibiotic resistance in soil bacterial populations. Plant roots influencing the soil environment and its microflora by exudation of growth substrates might considerably increase this effect. In this study, the effects of manure from pigs treated with sulfadiazine (SDZ), here called SDZ manure, on the abundance and transferability of sulfonamide resistance genes sul1 and sul2 in the rhizosphere of maize and grass were compared to the effects in bulk soil in a field experiment. In plots that repeatedly received SDZ manure, a significantly higher abundance of both sul genes was detected compared to that in plots where manure from untreated pigs was applied. Significantly lower abundances of sul genes relative to bacterial ribosomal genes were encountered in the rhizosphere than in bulk soil. However, in contrast to results for bulk soil, the sul gene abundance in the SDZ manure-treated rhizosphere constantly deviated from control treatments over a period of 6 weeks after manuring, suggesting ongoing antibiotic selection over this period. Transferability of sulfonamide resistance was analyzed by capturing resistance plasmids from soil communities into Escherichia coli. Increased rates of plasmid capture were observed in samples from SDZ manure-treated bulk soil and the rhizosphere of maize and grass. More than 97% of the captured plasmids belonged to the LowGC type (having low G+C content), giving further evidence for their important contribution to the environmental spread of antibiotic resistance. In conclusion, differences between bulk soil and rhizosphere need to be considered when assessing the risks associated with the spreading of antibiotic resistance. PMID:23315733

Trimethoprim-sulfonamide Use during the First Trimester of Pregnancy and the Risk of Congenital Anomalies

PubMed Central

Hansen, Craig; Andrade, Susan E.; Freiman, Heather; Dublin, Sascha; Haffenreffer, Katie; Cooper, William O.; Cheetham, T.Craig; Toh, Sengwee; Li, De-Kun; Raebel, Marsha A.; Kuntz, Jennifer L.; Perrin, Nancy; Rosales, A.Gabriela; Carter, Shelley; Pawloski, Pamala A.; Maloney, Elizabeth M.; Graham, David J.; Sahin, Leyla; Scott, Pamela E.; Yap, John; Davis, Robert

2016-01-01

Background Sulfonamide antibacterials are widely used in pregnancy, but evidence about their safety is mixed. The objective of this study was to assess the association between first-trimester sulfonamide exposure and risk of specific congenital malformations. Methods Mother-infant pairs were selected from a cohort of 1.2 million liveborn deliveries (2001–2008) at 11 US health plans comprising the Medication Exposure in Pregnancy Risk Evaluation Program (MEPREP). Mothers with first-trimester trimethoprim-sulfonamide (TMP-SUL) exposures were randomly matched 1:1 to 1) a primary comparison group: mothers exposed to penicillins and/or cephalosporins, and 2) a secondary comparison group: mothers with no dispensing of an antibacterial, antiprotozoal, or antimalarial medication during the same time period. The outcomes were cardiovascular abnormalities, cleft palate/lip, clubfoot, and urinary tract abnormalities. Results We first identified 7,615 infants in the TMP-SUL exposure group, of which 7595 (99%) were exposed to a combination of TMP-SUL and the remaining 1% to sulfonamides alone. After matching (1:1) to the comparator groups and only including those with complete data on covariates there were 20,064 (n=6,688 per group) in the primary analyses. Overall, cardiovascular defects (1.52%) were the most common and cleft lip/palate (0.10%) the least common that were evaluated. Compared to penicillin/cephalosporin exposure, and no antibacterial exposure, TMP-SUL exposure was not associated with statistically significant elevated risks for cardiovascular, cleft lip/palate, clubfoot, or urinary system defects. Conclusions First trimester TMP-SUL exposure was not associated with a higher risk of the congenital anomalies studied, compared to exposure to penicillins and/or cephalosporins, or no exposure to antibacterials. PMID:26599424

An empirical assessment of the Healthy Early Childhood Program in Rio Grande do Sul State, Brazil.

PubMed

Ribeiro, Felipe Garcia; Braun, Gisele; Carraro, André; Teixeira, Gibran da Silva; Gigante, Denise Petrucci

2018-01-01

We investigate the effect of a family-based primary health care program (Healthly Early Childhood Program) on infant mortality in the state of Rio Grande do Sul, Brazil. We estimate infant mortality's counterfactual trajectories using the differences-in-differences approach, combined with the use of longitudinal data for all municipalities in the state of Rio Grande do Sul. Our main result is that the program reduced the number of deaths caused by external causes. The length of exposure to the program seems to potentiate the effects. For the number of deaths by general causes, there is no evidence of impact. Our findings are consistent with the nature of the program that aims to improve adults care with children. The Healthly Early Childhood Program is effective in reducing the number of avoidable deaths in infants.

Comparison of organochlorine chemical body burdens of female breast cancer cases with cancer free women in Rio Grande do Sul, Brazil--Pilot Study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Erdmann, C.A.; Petreas, M.X.; Caleffi, M.

This pilot study collected preliminary data to examine known and suspected breast cancer risk factors among women living in rural and urban areas in the state of Rio Grande do Sul, Brazil by questionnaire. In addition, the body burden levels of a panel of organochlorines was measured in a small clinic-based prospective sample.

Towards a Pedagogy of a New Social Contract: Lessons from Participatory Budgeting in the State of Rio Grande do Sul (Brazil).

ERIC Educational Resources Information Center

Streck, Danilo R.

This paper analyzes the pedagogical dimension within the process of participatory budgeting in the state of Rio Grande do Sul, Brazil, taking into consideration the local and regional culture, as well as the political milieu. The question is whether, in this social movement which involved around 400,000 people in 2001, signs can be identified that…

Towards a Pedagogy of a New Social Contract: Lessons from the Participatory Budget in the State of Rio Grande do Sul (Brazil)

ERIC Educational Resources Information Center

Streck, Danilo Romeu

2004-01-01

The paper analyses the pedagogical dimension of the process of Participatory Budgeting in the State of Rio Grande do Sul (Brazil), taking into consideration the local and regional culture as well as the wider political milieu. The question this paper engages with is whether, in this social movement involving around 400,000 people in 2001, there…

Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine.

PubMed

Heuer, Holger; Solehati, Qodiah; Zimmerling, Ute; Kleineidam, Kristina; Schloter, Michael; Müller, Tanja; Focks, Andreas; Thiele-Bruhn, Sören; Smalla, Kornelia

2011-04-01

Two soils were amended three times with pig manure. The abundance of sulfonamide resistance genes was determined by quantitative PCR 2 months after each application. In both soils treated with sulfadiazine-containing manure, the numbers of copies of sul1 and sul2 significantly increased compared to numbers after treatments with antibiotic-free manure or a control and accumulated with repeated applications.

[Incidence of meningitis caused by Haemophilus influenzae in the state of Rio Grande do Sul 1999-2010: impact of vaccination campaign].

PubMed

Schossler, João Guilherme Stadler; Beck, Sandra Trevisan; de Campos, Marli Matiko Anraku; Farinha, Lourdes Boufleur

2013-05-01

This article seeks to analyze and update the epidemiological situation of meningitis caused by Haemophilus influenzae type b in the past 10 years in the state of Rio Grande do Sul (RS). It is a retrospective, descriptive study, which used the data notification system of meningitis and vaccination campaign coverage, stored in the Epidemiological TABNET online database, for the period from 1999 to 2010. Cases notified and confirmed were used and the selection criteria were the year when the symptoms were detected, age, diagnosis, and evolution. Nineteen health centers in the state of Rio Grande do Sul were analyzed. The z-test was used to evaluate comparisons between the proportions. In the period studied, 3043 confirmed cases of bacterial meningitis were reported, of which 6.77% were caused by H. influenzae. The incidence and mortality rates of meningitis caused by H. influenzae, without taking age group into consideration, fell significantly (95.6%) after 1999. Children under one year old continue to be the most affected (52%), there being no change in lethality. The results presented revealed a positive impact of Hib vaccination strategies in the state of Rio Grande do Sul over the past ten years.

Oral health self-perception in quilombola communities in Rio Grande do Sul: a cross-sectional exploratory study.

PubMed

Bidinotto, Augusto Bacelo; D'Ávila, Otávio Pereira; Martins, Aline Blaya; Hugo, Fernando Neves; Neutzling, Marilda Borges; Bairros, Fernanda de Souza; Hilgert, Juliana Balbinot

2017-01-01

There's a shortage of evidence on the oral health of quilombolas. This study aims to describe oral health self-perception, as well as to verify its associated factors in quilombola communities in the state of Rio Grande do Sul. The data for this cross-sectional health survey were collected by application of a questionnaire. Since this study was part of a survey on nutritional security, the probabilistic cluster sample was estimated for the outcome of nutritional insecurity, comprising 583 individuals across quilombola communities in Rio Grande do Sul. The association between the outcome of negative oral health self-perception and sociodemographic, general health, and oral health variables was measured by prevalence ratios obtained through Poisson regressions with robust variance and 95% confidence intervals. Negative self-rated oral health was reported by 313 (53.1%) of the individuals. Satisfaction with chewing ability and satisfaction with oral appearance were associated with a higher prevalence of negative perception of oral health, while there was no association between the outcome and number of teeth. Use of alcohol had a borderline association with the outcome. Satisfaction with appearance and chewing ability are factors associated with oral-health self-perception of the quilombolas in Rio Grande do Sul.

Reservoir Considerations and Direct Uses of São Pedro do Sul Hydromineral and Geothermal Field, Northern Portugal

NASA Astrophysics Data System (ADS)

Ferreira Gomes, L. M.; Neves Trota, A. P.; Sousa Oliveira, A.; Soares Almeida, S. M.

2017-12-01

São Pedro do Sul Hydromineral and Geothermal Field, located in the northern interior zone of Portugal (Lafões zone), has the greatest widespread utilization of geothermal energy in Portugal mainland and is the most important thermal centre from the economical revenues point of view, obtained from direct and indirect utilization of the thermal water, mostly for wellness, health, and leisure of human beings. Recent utilization includes district and greenhouses heating and even cosmetic applications. The Hydromineral Field includes two exploitable zones: the Termas and Vau Poles. The waters are recognised for their mineral and medicinal effects, since the time of the Romans about 2000 years ago and, later on, on the 12th century, by the first King of Portugal, D. Afonso Henriques. The traditional spring and the 500 m well (AC1), located in the Termas Pole, currently supplies artesian hot water flow of about 16.9 L/s with a temperature of 67 °C. Despite the low flow rate of the actual two exploration wells drilled in the Vau Pole, the geothermal potential is high; a new deep well is planned to be drilled in this zone where is expected to obtain fluid temperature of around 75 °C. The occurrence of São Pedro do Sul mineral water, included in the sulphurous type waters, are linked to Hercynian granitoids, emplaced between 290 and 321 Myr. There is a close relationship between the placement of the main hot springs and the Verin-Chaves-Penacova fault, namely Verin (Spain), Chaves, Moledo, and S. Pedro do Sul (Portugal) hot springs. Heat flow generated at shallow crustal zones by the radiogenic host mineral of the granitic rocks, added to the deep Earth heat flow, heats the cold water inflow along fractures. Open fracture network along the main faults allows the hot fluids reach the surface, thus giving chance to the occurrence of hot springs and mineralized cold springs. Coupling between fracture opening and density difference between cold water inflow and hot water upflow is assumed to be the main driven factors that explain the occurrence of hot spring in regions with normal to slightly abnormal geothermal gradient. Actual thermal output of the captured fluid in the São Pedro do Sul Hydrothermal area is not fully used, namely in summer times. Thus the main focus for the concessionary includes the saturation of the actual Termas heat power capacity either in the SPA utilization and expansion for newer users in the district heating system. Further studies must be conducted in order to ascertain for possible source deep exploitation to refine quantitatively the São Pedro do Sul reservoir conceptual model in order to make sustainable wise management of this important natural resource, critical for the São Pedro do Sul municipality development and also contributing for the Portuguese sustainable economic growth. In this paper we give new insights for the knowledge of São Pedro do Sul Hydromineral and Geothermal Field, namely the geothermal reservoir, the concession hot water exploitation and future perspectives for upcoming sustainable developments of this valuable natural resource.

Longivena, a new robber-fly genus from Brazil (Diptera, Asilidae, Asilinae).

PubMed

Vieira, Rodrigo; Rafael, José Albertino

2014-01-01

Longivena gen. n. and five new species are described and illustrated from caatinga and cerrado habitats from Brazil: Longivenadigitata sp. n., type-species (Maranhão, Bahia, Minas Gerais and Mato Grosso do Sul states), Longivenabilobata sp. n. (Maranhão state), Longivenaflava sp. n. (Mato Grosso do Sul state), Longivenalimeiraoliverai sp. n. (Maranhão state), Longivenaspatulata sp. n. (Maranhão state). An illustrated key is also provided.

The Hemiptera type-material housed in the "Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul" of Porto Alegre, Brazil.

PubMed

Ruschel, Tatiana Petersen; Guidoti, Marcus; Barcellos, Aline

2013-01-01

We provide a commented and referenced list on the type material deposited in the "Museu de Ciencias Naturais, Fundação Zoobotânica do Rio Grande do Sul", Porto Alegre, Brazil. Geographic coordinates are available on a digital repository for free access. High-resolution images of the specimens are available under request.

Rhipicephalus sanguineus (ACARI: IXODIDAE) BITING A HUMAN BEING IN PORTO ALEGRE CITY, RIO GRANDE DO SUL, BRAZIL

PubMed Central

MENTZ, Márcia Bohrer; TROMBKA, Marcelo; da SILVA, Guilherme Liberato; SILVA, Carlos Eugênio

2016-01-01

We report the finding of a female brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae) on the scalp of a male patient in Porto Alegre, Rio Grande do Sul, Brazil. Human parasitism by this tick is rare and has seldomly been reported in the literature, despite its recognized importance since it can act as a vector of Rickettsia rickettsii, the agent of spotted fever. PMID:27074329

Synopsis of Dorstenia (Moraceae) in Rio Grande do Sul, Southern Brazil.

PubMed

Boeni, Bruna De Oliveira; Singer, Rodrigo Bustos

2015-01-01

A taxonomic synopsis of Dorstenia (Moraceae) in Rio Grande do Sul (RS), Southern Brazil, is presented. Three species were recorded: D. brasiliensis, D. carautae, a new record for the state of RS, and D. tenuis. All species are described and illustrated through detailed photos of living specimens. A taxonomic key to separate the species, as well as details on distribution, overall phenology, habitat, conservation status and ecology are presented.

Rhipicephalus sanguineus (ACARI: IXODIDAE) BITING A HUMAN BEING IN PORTO ALEGRE CITY, RIO GRANDE DO SUL, BRAZIL.

PubMed

Mentz, Márcia Bohrer; Trombka, Marcelo; Silva, Guilherme Liberato da; Silva, Carlos Eugênio

2016-01-01

We report the finding of a female brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae) on the scalp of a male patient inPorto Alegre, Rio Grande do Sul, Brazil. Human parasitism by this tick is rare and has seldomly been reported in the literature, despite its recognized importance since it can act as a vector of Rickettsia rickettsii, the agent of spotted fever.

Occurrence of lace bug Vatiga illudens and Vatiga manihotae (Hemiptera: Tingidae) in Mato Grosso do Sul, midwestern Brazil.

PubMed

Bellon, Patrícia P; Wengrat, Ana P G S; Kassab, Samir O; Pietrowski, Vanda; Loureiro, Elisângela S

2012-09-01

Nymphs and adults of the lace bug (Hemiptera: Tingidae) have been found in cassava crops (Manihot esculenta) in Mato Grosso do Sul State, Brazil. The insects were collected in the field and taken to the laboratory where they were identified based on some morphological traits of the species Vatiga manihotae (Drake) and V. illudens (Drake), which are first reported in the aforementioned state.

Rhipicephalus sanguineus sensu lato (Ixodidae) in synantropic rodents in Rio Grande do Sul, Brazil.

PubMed

Winkel, Kathleen Tavares; Ribeiro, Paulo Bretanha; Antunes, Lidiane Oliveira; Cárcamo, Marcial Corrêa; Vianna, Elvia Elena Silveira

2014-01-01

Rhipicephalus sanguineus, the brown dog tick, is responsible for maintaining and transmitting various pathogens, both in animals and human beings, and it is of great sanitary importance. This communication reports the first occurrence of Rhipicephalus sanguineus sensu lato parasitizing Rattus norvegicus in the state of Rio Grande do Sul, Brazil, and it is also the first record of this tick species parasitizing Rattus rattus in Brazil. The rodents were captured from the port area, located in the city of Pelotas, Rio Grande do Sul, Brazil. We collected 6 larvae of this tick species from 2 male R. rattus individuals, and 3 larvae from 2 female R. norvegicus individuals; parasitized specimens of both rodent species were captured from different sites within the experimental area. This record broadens the number of Rhipicephalus sanguineus sensu lato hosts in urban areas, indicating the need for continued monitoring on population density for both R. sanguineus and synanthropic rodents.

Molecular identification of Hepatozoon canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil.

PubMed

Ramos, Carlos Alberto do Nascimento; Babo-Terra, Verônica Jorge; Pedroso, Thatianna Camillo; Souza Filho, Antônio Francisco; de Araújo, Flábio Ribeiro; Cleveland, Herbert Patric Kellermann

2015-01-01

The aim of this study was to investigate the occurrence of Hepatozoon species infecting dogs in the municipality of Campo Grande, Mato Grosso do Sul (MS), Brazil, using blood samples (n = 165) drawn from dogs. The species Hepatozoon canis was identified in 3.63% of the tested animals using molecular tools. Further studies are needed to determine the clinical relevance of this infection and the main arthropod vectors involved in its transmission.

An application of cluster analysis for determining homogeneous subregions: The agroclimatological point of view. [Rio Grande do Sul, Brazil

NASA Technical Reports Server (NTRS)

Parada, N. D. J. (Principal Investigator); Cappelletti, C. A.

1982-01-01

A stratification oriented to crop area and yield estimation problems was performed using an algorithm of clustering. The variables used were a set of agroclimatological characteristics measured in each one of the 232 municipalities of the State of Rio Grande do Sul, Brazil. A nonhierarchical cluster analysis was used and the pseudo F-statistics criterion was implemented for determining the "cut point" in the number of strata.

First record of the Lesser Snouted Treefrog Scinax nasicus (Cope, 1862) in Brazilian coast and new species records for the state of Rio Grande do Sul.

PubMed

Dalmolin, D A; Rosa, F O; Freire, M D; Fonte, L F M; Machado, I F; Paula, C N; Loebmann, D; Périco, E

2017-01-01

Herein, we provide new occurrence records of Scinax nasicus (Cope, 1862) for the state of Rio Grande do Sul, Southern Brazil. All new records here provide are located on Southern half of the state. Besides this, we provide the first record for species in Brazilian coastal zone. Those records improve considerably our knowledge regarding species distribution in Southern Brazil.

Pharmacokinetics and Pharmacodynamics of Amoxicillin-Sulbactam, a Novel Aminopenicillin–β-Lactamase Inhibitor Combination, against Escherichia coli

PubMed Central

Bantar, Carlos; Nicola, Federico; Arenoso, Hector J.; Galas, Marcelo; Soria, Liliana; Dana, Diego; Rossi, Alicia; Bianchini, Hebe; Jasovich, Abel

1999-01-01

We evaluated the pharmacokinetics of amoxicillin-sulbactam (AMX-SUL), a novel drug combination, and its pharmacodynamics against Escherichia coli in 12 volunteers receiving a single oral dose (1,000 mg). Peak serum bactericidal and urine inhibitory activities in most volunteers were observed against E. coli strains for which AMX-SUL MICs were low (2- to 4-mg/liter) (2 strains) and high (≥16-mg/liter) (47 strains), respectively. PMID:10348782

Shattering a myth - Whooping cough susceptible to antibiotics.

PubMed

Syed, Muhammad Ali; Jamil, Bushra; Bokhari, Habib

2016-05-01

Bordetella parapertussis is the causative agent of a milder form of pertussis or whooping cough. Little is reported about the antibiotic resistance patterns and mechanism of drug resistance of Bordetella parapertussis. The objective of this study has been to investigate antimicrobial resistance, distribution of integrons and presence of gene cassettes to quinolones (qnr) and sulfonamides (sul) among B. parapertussis strains' isolated from Pakistan. Thirty-five (35) samples were collected from various hospitals of Pakistan from children (median age 3 years) with pertussis-like symptoms, all were tested and confirmed to be B. Parapertussis. Resistance profile of Ampicillin, Cephalexin, Sulphamethoxazole, Chloramphenicol, Ofloxacin, Nalidixic acid, Gentamycin and Erythromycin were investigated through all samples. Majority of the isolates were found to be resistant to the afore-mentioned antibiotics except erythromycin. All isolates were resistant to quinolones phenotypically, but qnr genes were detected in only 25.7% (9/35) of isolates. On the other hand, 71.4% (25/35) isolates were resistant to sulfonamides phenotypically. From these 71% strains showing phenotypical resistance, 96% (24/25) were found to possess sul genes. Only two isolates were carrying class 1 integrons, which also harbored sul gene and qnr gene cassettes. It can be safely concluded that the phenotypic resistance patterns seemed mostly independent of presence of integrons. However, interestingly both integrons harboring strains were resistant to quinolones and sulfonamides and also possessed qnr and sul genes.

A historical legacy of antibiotic utilization on bacterial seed banks in sediments

PubMed Central

Junier, Thomas; Bayrychenko, Zhanna; Filippidou, Sevasti; Beck, Karin; Greub, Gilbert; Bürgmann, Helmut

2018-01-01

The introduction of antibiotics for both medical and non-medical purposes has had a positive effect on human welfare and agricultural output in the past century. However, there is also an important ecological legacy regarding the use of antibiotics and the consequences of increased levels of these compounds in the environment as a consequence of their use and disposal. This legacy was investigated by quantifying two antibiotic resistance genes (ARG) conferring resistance to tetracycline (tet(W)) and sulfonamide (sul1) in bacterial seed bank DNA in sediments. The industrial introduction of antibiotics caused an abrupt increase in the total abundance of tet(W) and a steady increase in sul1. The abrupt change in tet(W) corresponded to an increase in relative abundance from ca. 1960 that peaked around 1976. This pattern of accumulation was highly correlated with the abundance of specific members of the seed bank community belonging to the phylum Firmicutes. In contrast, the relative abundance of sul1 increased after 1976. This correlated with a taxonomically broad spectrum of bacteria, reflecting sul1 dissemination through horizontal gene transfer. The accumulation patterns of both ARGs correspond broadly to the temporal scale of medical antibiotic use. Our results show that the bacterial seed bank can be used to look back at the historical usage of antibiotics and resistance prevalence. PMID:29312823

Records of ticks on humans in Rio Grande do Sul state, Brazil.

PubMed

Reck, José; Souza, Ugo; Souza, Getúlio; Kieling, Eduardo; Dall'Agnol, Bruno; Webster, Anelise; Michel, Thais; Doyle, Rovaina; Martins, Thiago F; Labruna, Marcelo B; Marks, Fernanda; Ott, Ricardo; Martins, João Ricardo

2018-05-18

More than seventy tick species have been reported in Brazil. Despite the emergence of tick-borne diseases in Neotropical region, there are still limited data available on tick species parasitizing humans in Brazil. Rio Grande do Sul is the southernmost state of Brazil, comprising the only part of Brazilian territory inside the Pampa biome, as well as the transition between subtropical and temperate zones. Here, we report on human parasitism by ticks in Rio Grande do Sul state between 2004 and 2017. Seventy cases of human parasitism by ticks were recorded, with a total of 81 tick specimens collected. These included 11 tick species belonging to three genera of Ixodidae (hard-ticks), Amblyomma, Haemaphysalis and Rhipicephalus; and one genus of Argasidae, Ornithodoros. The most prevalent tick species associated to cases of human parasitism were Amblyomma parkeri (24%), Rhipicephalus sanguineus sensu lato (22%), Amblyomma aureolatum (15%) and Amblyomma ovale (12%). A spatial analysis showed two major hot spots of human parasitism by ticks in Rio Grande do Sul state. The findings of this study highlight the need for permanent monitoring of human parasitism by ticks in order to provide a better understanding of tick and tick-borne disease eco-epidemiology, and the early identification of potential cases of tick-borne diseases, particularly in spotted fever endemic regions. Copyright © 2018 Elsevier GmbH. All rights reserved.

Does human activity impact the natural antibiotic resistance background? Abundance of antibiotic resistance genes in 21 Swiss lakes.

PubMed

Czekalski, Nadine; Sigdel, Radhika; Birtel, Julia; Matthews, Blake; Bürgmann, Helmut

2015-08-01

Antibiotic resistance genes (ARGs) are emerging environmental contaminants, known to be continuously discharged into the aquatic environment via human and animal waste. Freshwater aquatic environments represent potential reservoirs for ARG and potentially allow sewage-derived ARG to persist and spread in the environment. This may create increased opportunities for an eventual contact with, and gene transfer to, human and animal pathogens via the food chain or drinking water. However, assessment of this risk requires a better understanding of the level and variability of the natural resistance background and the extent of the human impact. We have analyzed water samples from 21 Swiss lakes, taken at sampling points that were not under the direct influence of local contamination sources and analyzed the relative abundance of ARG using quantitative real-time PCR. Copy numbers of genes mediating resistance to three different broad-spectrum antibiotic classes (sulfonamides: sul1, sul2, tetracyclines: tet(B), tet(M), tet(W) and fluoroquinolones: qnrA) were normalized to copy numbers of bacterial 16S rRNA genes. We used multiple linear regression to assess if ARG abundance is related to human activities in the catchment, microbial community composition and the eutrophication status of the lakes. Sul genes were detected in all sampled lakes, whereas only four lakes contained quantifiable numbers of tet genes, and qnrA remained below detection in all lakes. Our data indicate higher abundance of sul1 in lakes with increasing number and capacity of wastewater treatment plants (WWTPs) in the catchment. sul2 abundance was rather related to long water residence times and eutrophication status. Our study demonstrates the potential of freshwater lakes to preserve antibiotic resistance genes, and provides a reference for ARG abundance from lake systems with low human impact as a baseline for assessing ARG contamination in lake water. Copyright © 2015 Elsevier Ltd. All rights reserved.

[Interaction Between Sulfonamide Antibiotics Fates and Chicken Manure Composting].

PubMed

Lin, Hui; Wang, Jian-mei; Sun, Wan-chun; Fu, Jian-rong; Chen, Hong-jin; Ma, Jun-wei

2016-05-15

Based on aerobic manure composting with or without the addition of a mixture of sulfadimethoxine SM2 and sulfamonomethoxine SMM (1:1, m/m), changes in the physic-chemical properties of manure compost, the microbial community physiological profiles, the antibiotics concentration and the abundances of five antibiotic resistance genes (ARGs) during the composting were tracked. The results indicated that the introduction of sulfonamide antibiotics led to inhibition on the basal respiration of manure compost during the early composting period, delayed the formation of thermophilic temperature and reduced the conversion of nutrients such as organic matter, ammonia nitrogen and nitrate nitrogen. Meanwhile, the introduction of sulfonamide antibiotics dramatically affected the physiological profile of microbial community in manure in the middle stage of composting. HPLC-MS/MS results showed that both SMM and SM2 in manure were completely degraded within 14 days, while the degradation rate of SMM was faster than that of SM2. For both composting treatments with or without addition of exogenous antibiotics, the relative abundance of sull and sul2 showed an initial decline in the first 14 or 21 days and a slight increase thereafter. The addition of exogenous antibiotics showed insignificant enhancement on increasing the relative abundance of sul1 and IntI1 in manure, but resulted in an apparent increase in sul2 relative abundance. Although the fates of tetQ and tetW during composting were different from that of sulfonamide ARGs, the introduction of sulfonamide antibiotics into manure increased the relative abundance of tetracycline ARGs. Redundancy analysis indicated that composting temperature correlated negatively with sul1, sul2 and IntI1 relative abundance in manure but had no obvious relationship with tetQ and tetW relative abundance. All the ARGs detected in this work correlated negatively with C/N ratio and the nitrate nitrogen concentration of manure compost but positively correlated with pH, moisture and ammonia nitrogen concentration of manure compost.

Modulatory effects of Ampicillin/Sulbactam on glial glutamate transporters and metabotropic glutamate receptor 1 as well as reinstatement to cocaine-seeking behavior.

PubMed

Hammad, Alaa M; Alasmari, Fawaz; Althobaiti, Yusuf S; Sari, Youssef

2017-08-14

Glutamatergic system has an important role in cocaine-seeking behavior. Studies have reported that chronic exposure to cocaine induces downregulation of glutamate transporter-1 (GLT-1) and cystine/glutamate exchanger (xCT) in the central reward brain regions. Ceftriaxone, a β-lactam antibiotic, restored GLT-1 expression and consequently reduced cue-induced reinstatement of cocaine-seeking behavior. In this study, we investigated the reinstatement to cocaine (20mg/kg, i.p.) seeking behavior using a conditioned place preference (CPP) paradigm in male alcohol-preferring (P) rats. In addition, we investigated the effects of Ampicillin/Sulbactam (AMP/SUL) (200mg/kg, i.p.), a β-lactam antibiotic, on cocaine-induced reinstatement. We also investigated the effects of AMP/SUL on the expression of glial glutamate transporters and metabotropic glutamate receptor 1 (mGluR1) in the nucleus accumbens (NAc) core and shell and the dorsomedial prefrontal cortex (dmPFC). We found that AMP/SUL treatment reduced cocaine-triggered reinstatement. This effect was associated with a decrease in locomotor activity. Moreover, GLT-1 and xCT were downregulated in the NAc core and shell, but not in the dmPFC, following cocaine-primed reinstatement. However, cocaine exposure increased the expression of mGluR1 in the NAc core, but not in the NAc shell or dmPFC. Importantly, AMP/SUL treatment normalized GLT-1 and xCT expression in the NAc core and shell; however, the drug normalized mGluR1 expression in the NAc core only. Additionally, AMP/SUL increased the expression of GLT-1 and xCT in the dmPFC as compared to the water naïve group. These findings demonstrated that glial glutamate transporters and mGluR1 in the mesocorticolimbic area could be potential therapeutic targets for the attenuation of reinstatement to cocaine-seeking behavior. Copyright © 2017 Elsevier B.V. All rights reserved.

Spatio-temporal drought characteristics of the tropical Paraiba do Sul River Basin and responses to the Mega Drought in 2014-2016

NASA Astrophysics Data System (ADS)

Nauditt, Alexandra; Metzke, Daniel; Ribbe, Lars

2017-04-01

The Paraiba do Sul River Basin (56.000 km2) supplies water to the Brazilian states Sao Paulo and Rio de Janeiro. Their large metropolitan areas were strongly affected by a Mega drought during the years 2014 and 2015 with severe implications for domestic water supply, the hydropower sector as well as for rural agricultural downstream regions. Longer drought periods are expected to become more frequent in the future. However, drought characteristics, low flow hydrology and the reasons for the recurrent water scarcity in this water abundant tropical region are still poorly understood. In order to separate the impact of human abstractions from hydro-climatic and catchment storage related hydrological drought propagation, we assessed the spatio-temporal distribution of drought severity and duration establishing relationships between SPI, SRI and discharge threshold drought anomalies for all subcatchments of the PdS based on a comprehensive hydro-meteorological data set of the Brazilian National Water Agency ANA. The water allocation model "Water Evaluation and Planning System (WEAP)" was established on a monthly basis for the entire Paraiba do Sul river basin incorporating human modifications of the hydrological system as major (hydropower) reservoirs and their operational rules, water diversions and major abstractions. It simulates reasonable discharges and reservoir levels comparable to the observed values. To evaluate the role of climate variability and drought responses for hydrological drought events, scenarios were developed to simulate discharge and reservoir level the impact of 1. Varying meteorological drought frequencies and durations and 2. Implementing operational rules as a response to drought. Uncertainties related to the drought assessment, modelling, parameter and input data were assessed. The outcome of this study for the first time provides an overview on the heterogeneous spatio-temporal drought characteristics of the Paraiba do Sul river basin and useful tools to support decision making and stakeholders as the River Basin Authority AGEVAP (Water Management Agency for the Paraiba do Sul).

Molecular epidemiological tracing of a cattle rabies outbreak lasting less than a month in Rio Grande do Sul in southern Brazil.

PubMed

Itou, Takuya; Fukayama, Toshiharu; Mochizuki, Nobuyuki; Kobayashi, Yuki; Deberaldini, Eduardo R; Carvalho, Adolorata A B; Ito, Fumio H; Sakai, Takeo

2016-02-12

Vampire bat-transmitted cattle rabies cases are typically encountered in areas where the disease is endemic. However, over the period of a month in 2009, an outbreak of cattle rabies occurred and then ended spontaneously in a small area of the Rio Grande do Sul State in southern Brazil. To investigate the epidemiological characteristics of this rabies outbreak in Rio Grande do Sul, 26 nucleotide sequences of rabies virus (RABV) genomes that were collected in this area were analyzed phylogenetically. Nucleotide sequence identities of the nucleoprotein gene and G-L intergenic region of the 26 RABVs were greater than 99.6 %. Phylogenetic analysis showed that all RABVs clustered with the vampire bat-related cattle RABV strains and that the RABVs were mainly distributed in southern Brazil. The findings of the present study suggested that a small population of rabid vampire bats carrying a single RABV strain produced a spatiotemporally restricted outbreak of cattle rabies in southern Brazil.

[Job stress in agents at the socio-educational service centers in the state of Rio Grande do Sul].

PubMed

Greco, Patrícia Bitencourt Toscani; Magnago, Tânia Solange Bosi de Souza; Beck, Carmem Lúcia Colomé; Urbanetto, Janete de Souza; Prochnow, Andrea

2013-03-01

The study was both to understand the association of work stress, socio-demographic and labor characteristics, habits and working conditions of the Socio-educational agents in the state of Rio Grande do Sul Brazil. It was a cross-sectional study with 881 agents of the Socio-educational Service Centers in the state of Rio Grande do Sul. The Brazilian version of the Job Stress Scale for assessment of work stress has been applied. Were classified in a situation of high strain 19.2% of the agents. The following factors were related to job stress, the need for counseling lack of leisure time, day shift work, dissatisfaction with the workplace, the need for absence from work due to health problems and insufficient scale work. There is a need to further research working conditions and execution of Occupational Health Service acting in order to minimize the effects of psychological demands at work of a socio-educational agent

Two new species of Hyalella (Crustacea, Amphipoda, Hyalellidae) from state of Rio Grande do Sul, Southern Brazil.

PubMed

Streck, Morgana Tais; Cardoso, Giovanna Monticelli; Rodrigues, Stella Gomes; Graichen, Daniel Angelo Sganzerla; Castiglioni, Daniela DA Silva

2017-10-17

There are 68 known species of Hyalella worldwide, with 23 occurring in Brazil. The state of Rio Grande do Sul, Southern Brazil, has the largest diversity of the genus in the country, with nine species recorded. The current study aimed to describe two new species of Hyalella from state of Rio Grande do Sul, both of them in the Northwest region of the state, one found in a small spring and another in an artificial pond. Hyalella georginae n. sp. presents several clusters of simple setae on antenna 2, maxilliped very slender, gnathopod 2 dactylus not reaching the lobe of propodus, pleopods rami with short plumose setae and a peculiar pattern of setae on uropods and telson. Hyalella gauchensis n. sp. presents antenna 2 with few setae, maxilliped very slender, gnathopod 2 dactylus reaching the lobe of propodus and pleopods rami with long plumose setae. From this work, the number of Hyalella species found in Brazil increases to 25 and 70 for the genus.

Spatial analysis of American Visceral Leishmaniasis in Mato Grosso do Sul State, Central Brazil.

PubMed

Correa Antonialli, Suely Aparecida; Torres, Thais Gisele; Paranhos Filho, Antonio Conceição; Tolezano, José Eduardo

2007-05-01

To map American Visceral Leishmaniasis (AVL) in Mato Grosso do Sul State (Central Brazil). The distribution of AVL was mapped, using the Geographic Information System. The disease was endemic to the Corumbá Region from 1913 to 1998. Spatial and temporal analysis indicated that the expansion route and dissemination through the State of the disease has been from west to east, coinciding with three different human interventions; two of them, a federal highway and a rail-road, were constructed in the early twentieth century, from east to west, from Bauru city, in São Paulo State, to Corumbá city, in Mato Grosso do Sul State. The third anthropogenic intervention was the construction of a gas pipeline that started in 1998, and attracted thousands of workers. This construction route has the same direction, west to east, and timescale as the observed expansion and dissemination of AVL. The results relate the expansion of the disease to intense human traffic along the route of spread.

[Demographic characteristics and mortality among indigenous peoples in Mato Grosso do Sul State, Brazil].

PubMed

Ferreira, Maria Evanir Vicente; Matsuo, Tiemi; Souza, Regina Kazue Tanno de

2011-12-01

The present study aimed to assess mortality rates and related demographic factors among indigenous peoples in the State of Mato Grosso do Sul, Central-West Brazil, compared to the State's general population. Mortality rates were estimated based on data obtained from the Health Care Database for Indigenous Peoples and monthly patient care records as well as demographic data from the Brazilian Unified National Health System (SUS) and mortality data from the SUS Mortality Database. Compared to the overall population, among indigenous peoples there were proportionally more individuals under 15 years of age and fewer elderly, besides higher mortality rates at early ages and from infectious and parasitic diseases. Indigenous men showed significantly higher mortality rates from external causes and respiratory and infectious diseases, while among women the mortality rates from external causes and infectious diseases were higher. Suicide rates among young indigenous individuals were also particularly alarming. Indigenous people's health conditions are worse than those of the general population in Mato Grosso do Sul.

Wild animals as sentinels of Paracoccidioides brasiliensis in the state of Rio Grande do Sul, Brazil.

PubMed

Albano, A P N; Klafke, G B; Brandolt, T M; Da Hora, V P; Minello, L F; Jorge, S; Santos, E O; Behling, G M; Camargo, Z P; Xavier, M O; Meireles, M C A

2014-04-01

Paracoccidioides brasiliensis, a dimorphic pathogenic fungus, causes the principal form of systemic mycosis in Brazil. The literature furnishes only limited data on the ecology of this fungus in the state of Rio Grande do Sul, the southernmost state of Brazil. The purpose of this study was to evaluate the prevalence of fungal infection in wild animals, using serological tests and using the animals as sentinels of the presence of P. brasiliensis in three specified mesoregions of Rio Grande do Sul. A total of 128 wild animals from the three mesoregions were included in the study. The serum samples were evaluated by immunodiffusion and the enzyme-linked immunosorbent assay (ELISA) technique to detect anti-gp43 antibodies from P. brasiliensis. Two conjugates were tested and compared with the ELISA technique. Although no positive samples were detected by immunodiffusion, 26 animals (20%), belonging to 13 distinct species, were found to be seropositive by the ELISA technique. The seropositive animals were from two mesoregions of the state. The results were similar according to the gender, age, and family of the animals, but differed significantly according to the conjugate used (p < 0.001), showing more sensitivity to protein A-peroxidase than to protein G-peroxidase. The finding that wild animals from the state of Rio Grande do Sul are exposed to P. brasiliensis suggests that the fungus can be found in this region despite the often-rigorous winters, which frequently include below-freezing temperatures.

Irrigated rice area estimation using remote sensing techniques: Project's proposal and preliminary results. [Rio Grande do Sul, Brazil

NASA Technical Reports Server (NTRS)

Parada, N. D. J. (Principal Investigator); Deassuncao, G. V.; Moreira, M. A.; Novaes, R. A.

1984-01-01

The development of a methodology for annual estimates of irrigated rice crop in the State of Rio Grande do Sul, Brazil, using remote sensing techniques is proposed. The project involves interpretation, digital analysis, and sampling techniques of LANDSAT imagery. Results are discussed from a preliminary phase for identifying and evaluating irrigated rice crop areas in four counties of the State, for the crop year 1982/1983. This first phase involved just visual interpretation techniques of MSS/LANDSAT images.

Assessment of the trophic status of four coastal lagoons and one estuarine delta, eastern Brazil.

PubMed

Cotovicz Junior, Luiz Carlos; Brandini, Nilva; Knoppers, Bastiaan Adriaan; Mizerkowski, Byanka Damian; Sterza, José Mauro; Ovalle, Alvaro Ramon Coelho; Medeiros, Paulo Ricardo Petter

2013-04-01

Anthropogenic eutrophication of aquatic ecosystems continues to be one of the major environmental issues worldwide and also of Brazil. Over the last five decades, several approaches have been proposed to discern the trophic state and the natural and cultural processes involved in eutrophication, including the multi-parameter Assessment of Estuarine Trophic Status (ASSETS) index model. This study applies ASSETS to four Brazilian lagoons (Mundaú, Manguaba, Guarapina, and Piratininga) and one estuarine delta (Paraíba do Sul River), set along the eastern Brazilian coast. The model combines three indices based on the pressure-state-response (PSR) approach to rank the trophic status and forecast the potential eutrophication of a system, to which a final ASSETS grade is established. The lagoons were classified as being eutrophic and highly susceptible to eutrophication, due primarily to their longer residence times but also their high nutrient input index. ASSETS classified the estuary of the Paraíba do Sul river with a low to moderate trophic state (e.g., largely mesotrophic) and low susceptibility to eutrophication. Its nutrient input index was high, but the natural high dilution and flushing potential driven by river flow mitigated the susceptibility to eutrophication. Eutrophication forecasting provided more favorable trends for the Mundaú and Manguaba lagoons and the Paraíba do Sul estuary, in view of the larger investments in wastewater treatment and remediation plans. The final ASSETS ranking system established the lagoons of Mundaú as "moderate," Manguaba as "bad," Guarapina as "poor," and Piratininga as "bad," whereas the Paraíba do Sul River Estuary was "good."

Antibiotic resistance genes in municipal wastewater treatment systems and receiving waters in Arctic Canada.

PubMed

Neudorf, Kara D; Huang, Yan Nan; Ragush, Colin M; Yost, Christopher K; Jamieson, Rob C; Truelstrup Hansen, Lisbeth

2017-11-15

Domestic wastewater discharges may adversely impact arctic ecosystems and local indigenous people, who rely on being able to hunt and harvest food from their local environment. Therefore, there is a need to develop efficient wastewater treatment plants (WWTPs), which can be operated in remote communities under extreme climatic conditions. WWTPs have been identified as reservoirs of antibiotic resistance genes (ARGs). The objective of this work was to quantify the presence of nine different ARG markers (int1, sul1, sul2, tet(O), erm(B), mecA, bla CTX-M , bla TEM , and qnr(S)) in two passive systems (waste stabilization ponds [WSPs]) and one mechanical filtration plant operating in two smaller and one large community, respectively, in Nunavut, Canada. Measurement of water quality parameters (carbonaceous oxygen demand, ammonia, total suspended solids, Escherichia coli and total coliforms) showed that the WWTPs provided only primary treatment. Low levels of the ARGs (2logcopies/mL) were observed in the effluent, demonstrating that bacteria residing in three northern WWTPs harbour ARGs conferring resistance to multiple clinically-relevant classes of antibiotics. Our results indicate that long-term storage in WSPs benefitted removal of organic material and some ARGs. However, one WSP system showed evidence of the enrichment of sul1, sul2, mecA, tet(O) and qnr(S). Further research is needed to fully understand if these ARG releases pose a risk to human health, especially in the context of traditional hunting and fishing activities. Copyright © 2017 Elsevier B.V. All rights reserved.

Abundances of Tetracycline, Sulphonamide and Beta-Lactam Antibiotic Resistance Genes in Conventional Wastewater Treatment Plants (WWTPs) with Different Waste Load

PubMed Central

Voolaid, Veiko; Ritz, Christian; Tenson, Tanel; Virta, Marko; Kisand, Veljo

2014-01-01

Antibiotics and antibiotic resistant bacteria enter wastewater treatment plants (WWTPs), an environment where resistance genes can potentially spread and exchange between microbes. Several antibiotic resistance genes (ARGs) were quantified using qPCR in three WWTPs of decreasing capacity located in Helsinki, Tallinn, and Tartu, respectively: sulphonamide resistance genes (sul1 and sul2), tetracycline resistance genes (tetM and tetC), and resistance genes for extended spectrum beta-lactams (blaoxa-58, blashv-34, and blactx-m-32). To avoid inconsistencies among qPCR assays we normalised the ARG abundances with 16S rRNA gene abundances while assessing if the respective genes increased or decreased during treatment. ARGs were detected in most samples; sul1, sul2, and tetM were detected in all samples. Statistically significant differences (adjusted p<0.01) between the inflow and effluent were detected in only four cases. Effluent values for blaoxa-58 and tetC decreased in the two larger plants while tetM decreased in the medium-sized plant. Only blashv-34 increased in the effluent from the medium-sized plant. In all other cases the purification process caused no significant change in the relative abundance of resistance genes, while the raw abundances fell by several orders of magnitude. Standard water quality variables (biological oxygen demand, total phosphorus and nitrogen, etc.) were weakly related or unrelated to the relative abundance of resistance genes. Based on our results we conclude that there is neither considerable enrichment nor purification of antibiotic resistance genes in studied conventional WWTPs. PMID:25084517

Effect of manure application on abundance of antibiotic resistance genes and their attenuation rates in soil: field-scale mass balance approach.

PubMed

Fahrenfeld, Nicole; Knowlton, Katharine; Krometis, Leigh Anne; Hession, W Cully; Xia, Kang; Lipscomb, Emily; Libuit, Kevin; Green, Breanna Lee; Pruden, Amy

2014-01-01

The development of models for understanding antibiotic resistance gene (ARG) persistence and transport is a critical next step toward informing mitigation strategies to prevent the spread of antibiotic resistance in the environment. A field study was performed that used a mass balance approach to gain insight into the transport and dissipation of ARGs following land application of manure. Soil from a small drainage plot including a manure application site, an unmanured control site, and an adjacent stream and buffer zone were sampled for ARGs and metals before and after application of dairy manure slurry and a dry stack mixture of equine, bovine, and ovine manure. Results of mass balance suggest growth of bacterial hosts containing ARGs and/or horizontal gene transfer immediately following slurry application with respect to ermF, sul1, and sul2 and following a lag (13 days) for dry-stack-amended soils. Generally no effects on tet(G), tet(O), or tet(W) soil concentrations were observed despite the presence of these genes in applied manure. Dissipation rates were fastest for ermF in slurry-treated soils (logarithmic decay coefficient of -3.5) and for sul1 and sul2 in dry-stack-amended soils (logarithmic decay coefficients of -0.54 and -0.48, respectively), and evidence for surface and subsurface transport was not observed. Results provide a mass balance approach for tracking ARG fate and insights to inform modeling and limiting the transport of manure-borne ARGs to neighboring surface water.

Tet and sul antibiotic resistance genes in livestock lagoons of various operation type, configuration, and antibiotic occurrence

USGS Publications Warehouse

McKinney, C.W.; Loftin, K.A.; Meyer, M.T.; Davis, J.G.; Pruden, A.

2010-01-01

Although livestock operations are known to harbor elevated levels of antibiotic resistant bacteria, few studies have examined the potential of livestock waste lagoons to reduce antibiotic resistance genes (ARGs). The purpose of this study was to determine the prevalence and examine the behavior of tetracycline [tet(O) and tet(W)] and sulfonamide [sul(I) and su/(II)] ARGsin a broad cross-section of livestock lagoons within the same semiarid western watershed. ARGs were monitored for one year in the water and the settled solids of eight lagoon systems by quantitative polymerase chain reaction. In addition, antibiotic residues and various bulk water quality constituents were analyzed. It was found that the lagoons of the chicken layer operation had the lowest concentrations of both tet and sul ARGs and low total antibiotic concentrations, whereas su ARGs were highest in the swine lagoons, which generally corresponded to the highest total antibiotic concentrations. A marginal benefit of organic and small dairy operations also was observed compared to conventional and large dairies, respectively. In all lagoons, su ARGs were observed to be generally more recalcitrant than tet ARGs. Also, positive correlations of various bulk water quality constituents were identified with tet ARGs but not sul ARGs. Significant positive correlations were identified between several metals and tet ARGs, but Pearson's correlation coefficients were mostly lower than those determined between antibiotic residues and ARGs. This study represents a quantitative characterization of ARGs in lagoons across a variety of livestock operations and provides insight into potential options for managing antibiotic resistance emanating from agricultural activities. ?? 2010 American Chemical Society.

Bucrates lanista Rehn 1918 (Tettigoniidae: Conocephalinae): The First Record from the Brazilian Pantanal, the First Description of the Male, the First Karyotypic Report for the Genus, and the First Telomeric Hybridization of the Subfamily.

PubMed

Chamorro-Rengifo, Juliana; Olivier, Renan da Silva; Araujo, Douglas

2016-10-01

Bucrates lanista, the most southerly distributed species in the genus Bucrates Burmeister, was originally described from Brazil based on a female collected in the state of Rio Grande do Sul, but the species has not been recorded since 1918. In this work, we report that B. lanista inhabits the Pantanal Wetland in the state of Mato Grosso do Sul and, for the first time, describe the male. Individuals of B. lanista are gregarious and present a brown/green color dimorphism; this behavior and color variation are also observed in species of closely related genera. Individuals from the Pantanal vary slightly from those of Rio Grande do Sul. The karyotype was determined to be 2n♂ = 21 = 20 + X0 and 2n♀ = 22 = 20 + XX. The X chromosome is metacentric and the largest of the complement, and all of the autosomes are submetacentrics. All chromosomes solely present telomeric (TTAGG)n repeats at their ends, and some chromosomes present positive and negative DAPI bands.

Rhipicephalus (Boophilus) microplus in the western-central region of Rio Grande do Sul, Brazil: multiresistant tick.

PubMed

Machado, Fabrício Amadori; Pivoto, Felipe Lamberti; Ferreira, Maiara Sanitá Tafner; Gregorio, Fabiano de Vargas; Vogel, Fernanda Silveira Flores; Sangioni, Luís Antônio

2014-01-01

The aim of the present study was to assess the acaricide resistance of tick populations in the western-central region of Rio Grande do Sul (Brazil), which has not previously been reported. Fifty-four cattle farms were visited and specimens of Rhipicephalus (Boophilus) microplus were collected and subjected to the adult immersion test, using nine commercial acaricides in the amidine, pyrethroid and organophosphate groups. Climatic data, including monthly precipitation, were recorded. The results from the present study demonstrated that seven of the acaricides analyzed presented mean efficacy values of less than 95%, with large differences among the products tested. Nine of them exhibited satisfactory and unsatisfactory acaricide results on at least one farm. In conclusion, the farms located in the western-central region of Rio Grande do Sul, Brazil, exhibited populations of R. (Boophilus) microplus with variable degrees of susceptibility to different acaricides, thus suggesting that resistance to the active compounds exists. It is suggested that treatment protocols should be implemented at the beginning of winter and summer, using the acaricides that showed efficacy in the adult immersion test.

Type catalog from the fish collection of the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, Brazil.

PubMed

Bertaco, Vinicius A; Azevedo, Marco A

2018-03-07

The catalog of type material deposited at the fish collection of the Museu de Ciências Naturais (MCN), Fundação Zoobotânica do Rio Grande do Sul, includes 315 specimens from 96 lots, representing six holotypes and 309 paratypes of 34 nominal species. Currently, two of them are synonymy and one have a questionable taxonomic status. Corrections and additional information from the original descriptions are included in the remarks for each species or lot. Photos of holotypes and paratypes are presented.

Natural infection of gastrointestinal nematodes in long-nosed armadillos Dasypus novemcinctus Linnaeus, 1758 from Pantanal wetlands, Aquidauana sub-region, Mato Grosso do Sul State, with the description of Hadrostrongylus speciosum n. gen. et n. sp. (Molineidae: Anoplostrongylinae).

PubMed

Lux Hoppe, Estevam G; do Nascimento, Adjair Antonio

2007-03-15

This study evaluated the gastrointestinal helminth fauna of long-nosed armadillos, Dasypus novemcinctus, from the Pantanal wetlands, Aquidauana sub-region, Aquidauana County, Mato Grosso do Sul State, Brazil. Thirteen species of nematodes, comprising seven genera and four families, were recovered from their gastrointestinal tracts. The following descriptors of infection were determined: prevalence, variation of intensity, average intensity and abundance. Hadrostrongylus speciosum n. gen. et n. sp. is first described here.

[Violence and social distress among transgender persons in Santa Maria, Rio Grande do Sul State, Brazil].

PubMed

Souza, Martha Helena Teixeira de; Malvasi, Paulo; Signorelli, Marcos Claudio; Pereira, Pedro Paulo Gomes

2015-04-01

The authors conducted an ethnographic research with transgender persons in Santa Maria, Rio Grande do Sul State, Brazil, in 2012, using participant observation, semi-structured interviews, and following their everyday lives. These individuals invariably experienced physical and symbolic violence and the resulting distress, a condition they had to deal with in their careers and daily practices and tasks. The article discusses the violence experienced by transvestites (in the family, school, police precincts, and health services), specifically seeking to understand how such violence relates to their experiences with health services and how the latter respond.

Studies of Brazilian meteorites. XIII - Mineralogy, petrology, and chemistry of the Putinga, Rio Grande do Sul, chondrite

NASA Technical Reports Server (NTRS)

Keil, K.; Lange, D.; Ulbrich, M. N. C.; Gomes, C. B.; Jarosewich, E.; Roisenberg, A.; Souza, M. J.

1978-01-01

The Putinga, Rio Grande do Sul chondrite is described and classified as an L6. The mineral composition and some significant ratios of elements are reported, and the reasons for assignment to the L group and to petrologic type 6 are explained. The analysis suggests that maskelynite of oligoclase composition was formed by solid-state shock transformation of previously existing well-crystallized plagioclase at estimated shock pressures of about 250-350 kbar. This finding indicates that recrystallization (formation of well-crystallized oligoclase) preceded shock transformation formation of the maskelynite.

A biogeochemical and isotopic view of Nitrogen and Carbon in rivers of the Alto Paraíba do Sul basin, São Paulo State, Brazil

NASA Astrophysics Data System (ADS)

Ravagnani, E. D. C.; Coletta, L. D.; Lins, S. R. M.; Antonio, J.; Mazzi, E. A.; Rossete, A. L. M.; Andrade, T. M. B.; Martinelli, L. A.

2014-12-01

The magnitude of potential flows of elements in tropical ecosystems is not well represented in the literature, even being very important. The Paraíba do Sul River drains the three more economically developed states in Brazil: São Paulo, Minas Gerais and Rio de Janeiro and its basin is considered extremely altered. Despite its economic and social importance (~ 5.3 mi inhabitants), we don't know much about carbon and nitrogen transport into its rivers and how these are affected by soil use changes. This work aimed to investigate these nutrients, using an isotopic and a biogeochemical approach, at some third order (Paraibuna, Paraitinga and Paraíba do Sul), second and first order rivers, all inserted at the Alto Paraíba do Sul Basin. In general, the low dissolved organic carbon, dissolved inorganic carbon, total dissolved nitrogen and inorganic N concentrations found in the first order rivers, showed the lower variation, despite changes in the soil use. Forested rivers presented higher DOC (3.3 mg.L-1) and TDN (14.2 mM) concentrations than the pasture rivers (2.6 mg.L-1 and 13.8 mM), while these presented higher DIC concentrations than those ones (90.2 mM and 71.2 mM). In third order rivers, the concentrations were also very low. Both carbon and nitrogen contents at the fine and coarse fractions of the suspended particulate material (SPM) were lower at Paraitinga and Paraiba do Sul Rivers. At the Paraibuna River, the fine fraction of SPM presented 25% of C concentration. The concentrations found at the coarse fraction were also higher at this river. The N concentrations were higher at the fine fraction and, consequently, this fraction presented higher C:N ratio. These observations allow us to say that the coarse fraction might be related to plant material, while the fine fraction is probably related to the soils. The δ13C in the SPM was lower in the Paraibuna River, probably due to the predominance of forest, while in the other ones pasture was the main soil use. The Paraibuna River presented the lower δ15N in the SPM results, similar to the results found at the Montane Forest, predominant at this basin, but are lower than the δ15N results found in the soils of the region. The higher results found in the Paraíba do Sul and Paraitinga Rivers may be explained by the highest N losses that occur in pasture grasses and soils.

Removal of selected PPCPs, EDCs, and antibiotic resistance genes in landfill leachate by a full-scale constructed wetlands system.

PubMed

Yi, Xinzhu; Tran, Ngoc Han; Yin, Tingru; He, Yiliang; Gin, Karina Yew-Hoong

2017-09-15

Landfill leachate could be a significant source of emerging contaminants (ECs) and antibiotic resistance genes (ARGs) into the environment. This study provides the first information on the occurrence of selected ECs and ARGs in raw leachate from 16-year old closed landfill site in Singapore. Among the investigated ECs, acetaminophen (ACT), bisphenol A (BPA), clofibric acid (CA), caffeine (CF), crotamiton (CTMT), diclofenac (DCF), N,N-diethyl-m-toluamide (DEET), gemfibrozil (GFZ), lincomycin (LIN), salicylic acid (SA), and sulfamethazine (SMZ) were the most frequently detected compounds in raw landfill leachate. The concentrations of detected ECs in raw landfill leachate varied significantly, from below quantification limit to 473,977 ng/L, depending on the compound. In this study, Class I integron (intl1) gene and ten ARGs were detected in raw landfill leachate. Sulfonamide resistance (sul1, sul2, and dfrA), aminoglycoside resistance (aac6), tetracycline resistance (tetO), quinolone resistance (qnrA), and intl1 were ubiquitously present in raw landfill leachate. Other resistance genes, such as beta-lactam resistance (blaNMD1, blaKPC, and blaCTX) and macrolide-lincosamide resistance (ermB) were also detected, detection frequency of <50%. The removal of target ECs and ARGs by a full-scale hybrid constructed wetland (CW) was also evaluated. The vast majority of ECs exhibited excellent removal efficiencies (>90%) in the investigated hybrid CW system. This hybrid CW system was also found to be effective in the reduction of several ARGs (intl1, sul1, sul2, and qnrA). Aeration lagoons and reed beds appeared to be the most important treatment units of the hybrid CW for removing the majority of ECs from the leachate. Copyright © 2017 Elsevier Ltd. All rights reserved.

Prevalence of sulfonamide and tetracycline resistance genes in drinking water treatment plants in the Yangtze River Delta, China.

PubMed

Guo, Xueping; Li, Jing; Yang, Fan; Yang, Jie; Yin, Daqiang

2014-09-15

The occurrence and distribution of antibiotic resistance genes (ARGs) in drinking water treatment plants (DWTPs) and finished water are not well understood, and even less is known about the contribution of each treatment process to resistance gene reduction. The prevalence of ten commonly detected sulfonamide and tetracycline resistance genes, namely, sul I, sul II, tet(C), tet(G), tet(X), tet(A), tet(B), tet(O), tet(M) and tet(W) as well as 16S-rRNA genes, were surveyed in seven DWTPs in the Yangtze River Delta, China, with SYBR Green I-based real-time quantitative polymerase chain reaction. All of the investigated ARGs were detected in the source waters of the seven DWTPs, and sul I, sul II, tet(C) and tet(G) were the four most abundant ARGs. Total concentrations of ARGs belonging to either the sulfonamide or tetracycline resistance gene class were above 10(5) copies/mL. The effects of a treatment process on ARG removal varied depending on the overall treatment scheme of the DWTP. With combinations of the treatment procedures, however, the copy numbers of resistance genes were reduced effectively, but the proportions of ARGs to bacteria numbers increased in several cases. Among the treatment processes, the biological treatment tanks might serve as reservoirs of ARGs. ARGs were found in finished water of two plants, imposing a potential risk to human health. The results presented in this study not only provide information for the management of antibiotics and ARGs but also facilitate improvement of drinking water quality. Copyright © 2014 Elsevier B.V. All rights reserved.

Inactivation of antibiotic resistance genes in municipal wastewater by chlorination, ultraviolet, and ozonation disinfection.

PubMed

Zhuang, Yao; Ren, Hongqiang; Geng, Jinju; Zhang, Yingying; Zhang, Yan; Ding, Lili; Xu, Ke

2015-05-01

This study investigated the inactivation of two antibiotic resistance genes (ARGs)-sul1 and tetG, and the integrase gene of class 1 integrons-intI1 by chlorination, ultraviolet (UV), and ozonation disinfection. Inactivation of sul1, tetG, and intI1 underwent increased doses of three disinfectors, and chlorine disinfection achieved more inactivation of ARGs and intI1 genes (chlorine dose of 160 mg/L with contact time of 120 min for 2.98-3.24 log reductions of ARGs) than UV irradiation (UV dose of 12,477 mJ/cm(2) for 2.48-2.74 log reductions of ARGs) and ozonation disinfection (ozonation dose of 177.6 mg/L for 1.68-2.55 log reductions of ARGs). The 16S rDNA was more efficiently removed than ARGs by ozone disinfection. The relative abundance of selected genes (normalized to 16S rDNA) increased during ozonation and with low doses of UV and chlorine disinfection. Inactivation of sul1 and tetG showed strong positive correlations with the inactivation of intI1 genes (for sul1, R (2)  = 0.929 with p < 0.01; for tetG, R (2)  = 0.885 with p < 0.01). Compared to other technologies (ultraviolet disinfection, ozonation disinfection, Fenton oxidation, and coagulation), chlorination is an alternative method to remove ARGs from wastewater effluents. At a chlorine dose of 40 mg/L with 60 min contact time, the selected genes inactivation efficiency could reach 1.65-2.28 log, and the cost was estimated at 0.041 yuan/m(3).

Estimativas de possiveis recursos de petroleo e gas na America Central e na America do Sul [Estimates of possible petroleum and gas resources in Central American and South America

USGS Publications Warehouse

Schenk, C.S.

2001-01-01

O U.S. Geological Survey recentemente completou estimativas de possíveis recursos de petróleo e gás em 130 áreas petrolíferas pré-determinadas no mundo (USGS, 2000). Vinte e três destas áreas ficam na América do Sul, na América Central, e no Caribe (fig. 1). Os resultados estão apresentados na tabela 1. Nas 23 áreas, estimamos um total de 105 BBO e um total de 487 TCFG. A região composta de América Central mais América do Sul ficou em terceiro lugar no mundo em termos de possíveis recursos de petróleo e gás. No primeiro lugar ficou o Oriente Médio e no segundo lugar ficou a antiga União Soviética (USGS, 2000). As áreas com maiores probabilidades de encontrar depósitos gigantes de petróleo e gás se localizam nas áreas do Oceano Atlântico começando com a Bacia de Santos no sul até a Bacia Guyana-Suriname no norte. As possibilidades de existirem depósitos gigantes são maiores nas áreas submersas do mar até profundidades de 3,600 m. Diversos depósitos gigantes de petróleo foram descobertos no mar na Bacia de Campos e ainda podem serem encontrados depósitos similares na Bacia de Campos e suas imediações.

The HLA-A, -B and -DRB1 polymorphism in a large dataset of South Brazil bone marrow donors from Rio Grande do Sul.

PubMed

Boquett, J A; Nunes, J M; Buhler, S; de Oliveira, M Z; Jobim, L F; Jobim, M; Fagundes, N J R; Schüler-Faccini, L; Sanchez-Mazas, A

2017-01-01

Human leukocyte antigen (HLA) genes are very informative in population genetics studies and their variability has been widely used to reconstruct the history of geographic and/or demographic expansions of human populations. The characterization of HLA diversity at the population level is also fundamental in clinical studies, particularly for bone marrow transplantation programs. In this study, we investigated the HLA molecular variation in Rio Grande do Sul, South Brazil, in order to identify possible regional differences across this state. More than 97,000 bone marrow donors were typed at the HLA- A, -B and -DRB1 loci and analyzed by considering two kinds of subdivisions based on both self-identified ethnicity and place of residence: (a) the official geographic subdivision defined by the Brazilian Institute of Geography and Statistics and (b) known information about the colonization history of the state. HLA allele and haplotype frequencies were estimated and compared among the defined subgroups. The results indicate a lack of correlation between genetic variation and geography and thus no clear HLA genetic structure based on geographic criteria. On the other hand, major differences were observed regarding ethnicity. In addition, local populations from Rio Grande do Sul were found to be genetically similar to their corresponding parental European populations from Germany, Italy and Portugal, as documented by historical data. Overall, this study provides a thorough characterization of the HLA genetic variation in Rio Grande do Sul and a better understanding of its demographic history, being most useful for the development of more efficient strategies in bone marrow donors' recruitment. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Competition between agricultural, urban, and sand-mining areas at the Paraíba do Sul basin in southeastern Brazil

NASA Astrophysics Data System (ADS)

Ronquim, Carlos C.; Cordeiro, Guilherme P. L.; Amorim, Mariana de; de C. Teixeira, Antônio H.; Leivas, Janice F.; Galdino, Sergio

2017-10-01

This work was performed in the Paraíba do Sul basin, within the limits of the São Paulo state, southeastern Brazil, in order to assess the dynamics of the land-use and land-cover changes at the Paraíba do Sul river's floodplains between 1985 and 2016. We focused on investigating the development of agricultural areas used for the production of wetland rice and of areas featuring artificial lakes produced by sand mining. We mapped the land cover in 1985 using images made by the Landsat 5 satellite's Moderate Resolution Imaging Spectroradiometer (MODIS) and Thematic Mapper (TM) sensors, which were segmented to produce vectors featuring homogeneous characteristics, and which were classified by means of visual interpretation. Similarly, we applied the maximum likelihood classification and used spectral curve inserts and adjustments to study and analyze the same area using a Landsat 8's Operational Land Imager (OLI) image made in 2016. Our results show significant reduction of areas used for rice crops, and increase in areas featuring sandmining pits. The rice crop areas decreased approximately 43% from 24,131.4 ha in 1985 to 13,789.8 ha in 2016. Over this 30-year period, the area covered by sand-mining lakes increased from 615 ha to 3,876 ha (+ 630%), and the number of lakes increased from 54 to 316. Sand mining and urbanization are the main factors causing the reduction in wetland rice areas. The absence of environmental management actions at the basin interferes with the rice production, which depends on the Paraíba do Sul river's floodplains.

Dopamine D2 receptor antagonist sulpiride enhances dexamethasone responses in the treatment of drug-resistant and metastatic breast cancer.

PubMed

Li, Jian; Yao, Qing-Yu; Xue, Jun-Sheng; Wang, Li-Jie; Yuan, Yin; Tian, Xiu-Yun; Su, Hong; Wang, Si-Yuan; Chen, Wen-Jun; Lu, Wei; Zhou, Tian-Yan

2017-09-01

Recent evidence shows that dopamine D2-like receptor (D2DR) antagonists, such as trifluoperazine and thioridazine, are effective for cancer therapy and inhibition of cancer stem-like cells (CSCs). In this study, we investigated the anti-cancer effects of combination therapy of dexamethasone (DEX) and sulpiride (SUL), an atypical antipsychotic, against drug-resistant and metastatic breast cancers and further explored the underlying mechanisms. Oral administration of SUL (25, 100 mg·kg -1 ·d -1 ) alone did not inhibit the tumor growth in human breast cancer MCF-7/Adr xenograft model, but dose-dependently decreased the proportion of CSCs in vitro and in vivo. In contrast, combination therapy of SUL (50 mg·kg -1 ·d -1 ) and DEX (8 mg·kg -1 ·d -1 ) markedly suppressed the tumor growth in MCF-7/Adr xenograft model with little systemic toxicity and lung metastasis in murine metastatic breast cancer 4T1 xenograft model. Among the metastasis-associated biomarkers analyzed, the combination therapy significantly decreased the levels of MMP-2, but increased E-cadherin levels in 4T1 xenograft tumors. Moreover, the combination therapy significantly inhibited the cell colony formation, migration and invasion of 4T1 and human breast cancer MDA-MB-231 cells in vitro. Addition of a specific D2DR agonist 7-OH-DPAT to the combination therapy reversed the enhanced anti-cancer effects in vivo and CSC population loss in tumor tissues. Our data demonstrate that SUL remarkably enhances the efficacy of DEX in the treatment of drug-resistant and metastatic breast cancer via the antagonism of D2DR, which might result from the eradication of CSCs.

Prognostic stratification model for patients with stage I non-small cell lung cancer adenocarcinoma treated with surgical resection without adjuvant therapies using metabolic features measured on F-18 FDG PET and postoperative pathologic factors.

PubMed

Kang, Yeon-Koo; Song, Yoo Sung; Cho, Sukki; Jheon, Sanghoon; Lee, Won Woo; Kim, Kwhanmien; Kim, Sang Eun

2018-05-01

In the management of non-small cell lung cancer (NSCLC), the prognostic stratification of stage I tumors without indication of adjuvant therapy, remains to be elucidated in order to better select patients who can benefit from additional therapies. We aimed to stratify the prognosis of patients with stage I NSCLC adenocarcinoma using clinicopathologic factors and F-18 FDG PET. We retrospectively enrolled 128 patients with stage I NSCLC without any high-risk factors, who underwent curative surgical resection without adjuvant therapies. Preoperative clinical and postoperative pathologic factors were evaluated by medical record review. Standardized uptake value corrected with lean body mass (SUL max ) was measured on F-18 FDG PET. Among the factors, independent predictors for recurrence-free survival (RFS) were selected using univariate and stepwise multivariate survival analyses. A prognostic stratification model for RFS was designed using the selected factors. Tumors recurred in nineteen patients (14.8%). Among the investigated clinicopathologic and FDG PET factors, SUL max on PET and spread through air spaces (STAS) on pathologic review were determined to be independent prognostic factors for RFS. A prognostic model was designed using these two factors in the following manner: (1) Low-risk: SUL max  ≤ 1.9 and no STAS, (2) intermediate-risk: neither low-risk nor high-risk, (3) high-risk: SUL max> 1.9 and observed STAS. This model exhibited significant predictive power for RFS. We showed that FDG uptake and STAS are significant prognostic markers in stage I NSCLC adenocarcinoma treated with surgical resection without adjuvant therapies. Copyright © 2018 Elsevier B.V. All rights reserved.

Female homicide in Rio Grande do Sul, Brazil.

PubMed

Leites, Gabriela Tomedi; Meneghel, Stela Nazareth; Hirakata, Vania Noemi

2014-01-01

This study aimed to assess the female homicide rate due to aggression in Rio Grande do Sul, Brazil, using this as a "proxy" of femicide. This was an ecological study which correlated the female homicide rate due to aggression in Rio Grande do Sul, according to the 35 microregions defined by the Brazilian Institute of Geography and Statistics (IBGE), with socioeconomic and demographic variables access and health indicators. Pearson's correlation test was performed with the selected variables. After this, multiple linear regressions were performed with variables with p < 0.20. The standardized average of female homicide rate due to aggression in the period from 2003 to 2007 was 3.1 obits per 100 thousand. After multiple regression analysis, the final model included male mortality due to aggression (p = 0.016), the percentage of hospital admissions for alcohol (p = 0.005) and the proportion of ill-defined deaths (p = 0.015). The model have an explanatory power of 39% (adjusted r2 = 0.391). The results are consistent with other studies and indicate a strong relationship between structural violence in society and violence against women, in addition to a higher incidence of female deaths in places with high alcohol hospitalization.

Trypanosoma cruzi strains from triatomine collected in Bahia and Rio Grande do Sul, Brazil.

PubMed

Ribeiro, Aline Rimoldi; Mendonça, Vagner José; Alves, Renata Tomé; Martinez, Isabel; Araújo, Renato Freitas de; Mello, Fernanda; da Rosa, João Aristeu

2014-04-01

Collection of triatomines in domestic, peridomestic and sylvatic environments in states of Bahia and Rio Grande do Sul, Northeastern and Southern Brazil respectively, and isolation of Trypanosoma cruzi strains. First, the captured triatomines were identified using insect identification keys, then their intestinal content was examined by abdominal compression, and the samples containing trypanosomatid forms were inoculated in LIT medium and Swiss mice. Six triatomine species were collected in cities in Bahia, namely Panstrongylus geniculatus (01), Triatoma melanocephala (11), T. lenti (94), T. pseudomaculata (02), T. sherlocki (26) and T. sordida (460), and two in cities in Rio Grande do Sul, namely T. circummaculata (11) and T. rubrovaria (115). Out of the specimens examined, T. cruzi was isolated from 28 triatomine divided into four different species: T. melanocephala (one), T. lenti (one), T. rubrovaria (16) and T. sordida (10). Their index of natural infection by T. cruzi was 6.4%. The isolation of T. cruzi strains from triatomines found in domestic and peridomestic areas shows the potential risk of transmission of Chagas disease in the studied cities. The maintenance of those T. cruzi strains in laboratory is intended to promote studies that facilitate the understanding of the parasite-vector-host relationship.

Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil.

PubMed

Albano, Ana Paula Neuschrank; Klafke, Gabriel Baracy; Brandolt, Tchana Martinez; Da Hora, Vanusa Pousada; Nogueira, Carlos Eduardo Wayne; Xavier, Melissa Orzechowski; Meireles, Mário Carlos Araújo

2015-06-01

Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil.

Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil

PubMed Central

Albano, Ana Paula Neuschrank; Klafke, Gabriel Baracy; Brandolt, Tchana Martinez; Da Hora, Vanusa Pousada; Nogueira, Carlos Eduardo Wayne; Xavier, Melissa Orzechowski; Meireles, Mário Carlos Araújo

2015-01-01

Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil. PMID:26273267

Contamination profiles of antibiotic resistance genes in the sediments at a catchment scale.

PubMed

Su, Hao-Chang; Pan, Chang-Gui; Ying, Guang-Guo; Zhao, Jian-Liang; Zhou, Li-Jun; Liu, You-Sheng; Tao, Ran; Zhang, Rui-Quan; He, Liang-Ying

2014-08-15

The aim of this study was to investigate the contamination profiles of tetracycline, sulfonamide, and macrolide resistance genes, as well as integrons in sediments of Dongjiang River basin of South China by real time quantitative polymerase chain reaction. sul2 was the most abundant resistance gene, with the average concentration of 6.97×10(8) copies/g and 1.00×10(8) copies/g in the dry and wet seasons, respectively, followed by ermF, sul3, sul1, intI1, tetA, ermB, tetX, tetM, tetQ, tetO, tetW, tetS, ermC, and tetB. The abundance of intI2 gene was the lowest in the sediment samples. Significant correlations existed between the ARGs and sediment properties as well as metals (Cu and Zn) and corresponding antibiotic classes, suggesting that the contamination of ARGs is related to chemical pollution of the sediments in the river basin. Principal component analysis showed distinct groupings of the sampling sites, reflecting that human activities are the key player in the dissemination of ARGs in the catchment environment. Copyright © 2014 Elsevier B.V. All rights reserved.

Fasciola hepatica IN BOVINES IN BRAZIL: DATA AVAILABILITY AND SPATIAL DISTRIBUTION

PubMed Central

Bennema, Sita C.; Scholte, Ronaldo Guilherme Carvalho; Molento, Marcelo Beltrão; Medeiros, Camilla; Carvalho, Omar dos Santos

2014-01-01

Fasciolosis is a disease of importance for both veterinary and public health. For the first time, georeferenced prevalence data of Fasciola hepatica in bovines were collected and mapped for the Brazilian territory and data availability was discussed. Bovine fasciolosis in Brazil is monitored on a Federal, State and Municipal level, and to improve monitoring it is essential to combine the data collected on these three levels into one dataset. Data were collected for 1032 municipalities where livers were condemned by the Federal Inspection Service (MAPA/SIF) because of the presence of F. hepatica. The information was distributed over 11 states: Espírito Santo, Goiás, Minas Gerais, Mato Grosso do Sul, Mato Grosso, Pará, Paraná, Rio de Janeiro, Rio Grande do Sul, Santa Catarina and São Paulo. The highest prevalence of fasciolosis was observed in the southern states, with disease clusters along the coast of Paraná and Santa Catarina and in Rio Grande do Sul. Also, temporal variation of the prevalence was observed. The observed prevalence and the kriged prevalence maps presented in this paper can assist both animal and human health workers in estimating the risk of infection in their state or municipality. PMID:24553606

Evaluation of natural foci of Panstrongylus megistus in a forest fragment in Porto Alegre, State of Rio Grande do Sul, Brazil.

PubMed

Santos, José Eloy Dos; Viola, Mariana Gubert; Lorosa, Elias Seixas; Machado, Evandro Marques de Menezes; Ruas Neto, Antonio Leite; Corseuil, Elio

2013-01-01

Panstrongylus megistus is commonly found in wild environments of the State of Rio Grande do Sul, Brazil. The aim of this study was to characterize the network of refuges used by triatomine in a forest fragment of Porto Alegre and to identify Trypanosoma cruzi infection, associated hosts and the epidemiological importance of both hosts and triatomines. Techniques including the spool-and-line method and active searching (transects) were used to identify natural foci. The food source for each triatomine was determined using the precipitin test, and the infection of marsupials was determined by xenodiagnosis. A total of 33 adults (domestic environment) and 27 nymphs (wild environment) of P. megistus were found in addition to 43 Didelphis albiventris specimens. The infection rates of triatomine adults, triatomine nymphs and opossums with T. cruzi I were 64%, 73% and 69%, respectively. Birds, rodents and opossums were the main resources used by triatomine. This work presents the first characterization of a natural focus of P. megistus in Rio Grande do Sul. The natural characteristics of this focus and its implication in the transmission of T. cruzi are discussed.

Genetic characterization of the mechanisms of resistance to amoxicillin/clavulanate and third-generation cephalosporins in Salmonella enterica from three Spanish hospitals.

PubMed

de Toro, María; Sáenz, Yolanda; Cercenado, Emilia; Rojo-Bezares, Beatriz; García-Campello, Marta; Undabeitia, Esther; Torres, Carmen

2011-09-01

The mechanisms of antimicrobial resistance were characterized in 90 Salmonella enterica isolates either resistant or with intermediate resistance to amoxicillin/clavulanate (AMC(R/I)) or resistant to third-generation cephalosporins (C3G(R)). These isolates were recovered in three Spanish hospitals during 2007-2009. The C3G(R) phenotype was expressed by three isolates that carried the following extended-spectrum β-lactamase genes: phage-associated bla(CTX M-10) in S. Virchow, bla(CTX-M-14a) surrounded by ISEcp1 and IS903 in S. Enteritidis, and bla(CTX-M-15) linked to ISEcp1 and orf477 in S. Gnesta (first description in this serotype). The AMC(R/I) phenotype was found in 87 isolates (79 S. Typhimurim, 7 S. Enteritidis, and one S. Thompson). The bla(PSE-1) gene, followed by bla(OXA-1) was mostly found among S. Typhimurim, and the bla(TEM-1) gene among S. Enteritidis. Three different gene combinations [bla(PSE-1) +floR+aadA2+sul+tet(G); bla(OXA-1) +catA+aadA1/strA-strB+sul+tet(B) and bla(TEM-1) + cmlA1+aadA/strA-strB+sul+tet(A)/tet(B) genes] were associated with the ampicillin-chloramphenicol-streptomycin-sulfonamides-tetracycline phenotype in 68 AMC(R/I) S. enterica isolates. Class 1 integrons were observed in 79% of the isolates and in most of them (45 isolates) two integrons including the aadA2 and bla(PSE-1) gene cassettes, respectively, were detected. The bla(OXA-1) +aadA1 arrangement was detected in 23 isolates, and the aac(6')-Ib-cr+bla(OXA-1) +catB3+arr3 in another one. Non-classic class 1 integrons were found in three isolates: dfrA12+orfF+aadA2+cmlA1+aadA1 (1 isolate), dfrA12+orfF+aadA2+ cmlA1+aadA1+qacH+IS440+sul3 (1 isolate) and dfrA12+orfF+aadA2+cmlA1+aadA1+qacH+IS440+ sul3+orf1+mef(B)Δ-IS26 (1 isolate). Taken together, these results underline the need for clinical concern regarding β-lactam resistance in Salmonella and thus for continuous monitoring.

Occurrence and distribution of antibiotics, antibiotic resistance genes in the urban rivers in Beijing, China.

PubMed

Xu, Yan; Guo, Changsheng; Luo, Yi; Lv, Jiapei; Zhang, Yuan; Lin, Haixia; Wang, Li; Xu, Jian

2016-06-01

The occurrence and distribution of sulfonamide and tetracycline, corresponding bacterial resistant rate and resistance genes (ARGs) and two integrase genes were investigated in seven urban rivers in Beijing, China. The total concentration of sulfonamide and tetracycline ranged from 1.3 × 10(1)-1.5 × 10(3) ng/L and 3.9 × 10(1)-5.4 × 10(4) ng/L for water, and 1.0 × 10(0)-2.7 × 10(2) and 3.1 × 10(1)-1.6 × 10(2) ng/g for sediment, respectively. The sul resistant rate was 2-3 times higher than tet resistant rate in both surface water and sediment. The average rate of sul resistance and tet resistance were up to 81.3% and 38.6% in surface water, 89.1% and 69.4% in the sediment, respectively. The sul1, tetA and tetE genes were predominant in term of the absolute abundance. The absolute abundance of ARGs in Wenyu River and Qinghe River, which were close to the direct discharging sites, were 5-50 times higher than those in the other investigated urban rivers, suggesting that the source release played an important role in the distribution of ARGs. The sul1 and sul2 genes had positive correlation (p < 0.05) with sulfonamides, and the tet resistance genes was significantly correlated with tetracyclines (p < 0.05), indicating that some ARGs and antibiotics in the urban rivers had identical sources of pollution. Considering principal component analysis, sampling sites (QH5, QH6, B1, B2, B3 and BX2) intimated that a complex interplay of processes govern fate and transport of ARGs in the junction of rivers. These results are significant to understand the fate, and the contribution of ARGs from the source release. In view of the large-scale investigation of urban rivers system in Beijing, it reflected the bacterial resistance in sewage drainage system. Such investigation highlights the management on controlling the pollutant release which was seemed as a major driving force for the maintenance and propagation of many ARGs during the development of urbanization in the future. Copyright © 2016 Elsevier Ltd. All rights reserved.

[Mental illness in women in Porto Alegre, Rio Grande do Sul, Brazil (1870-1910)].

PubMed

Vasconcellos, Cristiane Teresinha de Deus Virgili; Vasconcellos, Silvio José Lemos

2007-05-01

The relationship between female gender and mental illness is complex, remaining largely a product of women's social situation as daughters, wives, and mothers. The main objective of this article is to discuss the historical aspects related to mental illness in women in Porto Alegre, Rio Grande do Sul, Brazil, from 1870 to 1910. The authors consulted records from several so-called insane asylums as well as periodical articles published during the period. These documents provide good insight into how psychiatrists and lay society interpreted mental disorders in women. The research contributes to an understanding of the historical issues related to diagnosis of mental illness and the implications for current practice.

A Study of the Perceptions of Roles, Responsibilities, and Problem Areas during Facility Transition in the Military Construction Program.

DTIC Science & Technology

1987-09-01

priority based on the r sul-s of the rank order analysis of the combined USAF/COE Sa a jrouo n1 or :v.e USA’ dana qrouo. The COE data group sablisn...between the Resident Engineer and the DEEC is needed. O&M Manuals. The USAF conmnents anI concerns a bo,1t -ie &M M4ani’L3 4ere: 1. Som etnes g-ttinj the...teR-2sidJent Engineer- .ini tn- Chieff oDf Contract Man age-!n-enit ( DEEC is needed. Along the same line , onie COE comnmen t statedI that thne

Comparative morphology of immature stages of four species of Chinavia (Hemiptera, Pentatomidae), with a key to the species of Rio Grande do Sul, Brazil

PubMed Central

Fürstenau, Brenda Bianca Rodrigues Jesse; Schwertner, Cristiano Feldens; Grazia, Jocelia

2013-01-01

Abstract Chinavia Orian (1965) is one of the most diverse genera of Pentatomidae, distributed in the Afrotropical, Neotropical and Nearctic Regions. Thirty-two species are recorded for Brazil, some of them having potential economic impact because they are found on crops and referred to as pests. The morphology of the five nymphal instars of Chinavia armigera (Stål, 1859), Chinavia aseada (Rolston, 1983), Chinavia brasicola (Rolston, 1983) and Chinavia runaspis (Dallas, 1851) are described here. Through a comparative study, identification keys were developed to allow an early identification of the 12 Chinavia species of Rio Grande do Sul. PMID:24039512

Paraiba do Sul river delta, Brazil

NASA Image and Video Library

1996-01-20

STS072-738-019 (11-20 Jan. 1996) --- The Delta of the Paraiba do Sul River, northeast of Rio de Janeiro, Brazil, stands out in this 70mm frame exposed from the Earth-orbiting Space Shuttle Endeavour. The brown color of the river water and offshore sediment plume show that the river is in flood stage. This delta attracts much attention from orbit because of its prominent beach ridges either side of the river mouth. River sediment from inland supplies the material which is redistributed by coastal currents to form the parallel beach ridges. The lower 20 miles of the river appear in this scene. The river flows into the Atlantic in an easterly direction.

Referring Quality Assessment of Primary Health Care for Endocrinology in Rio Grande do Sul, Brazil.

PubMed

Monteiro Grendene, Gabriela; Szczecinski Rodrigues, Átila; Katz, Natan; Harzheim, Erno

2015-01-01

This paper presents results of an assessment of the quality research of endocrinology referrals in the public health system in the state of Rio Grande do Sul. From the analysis of 4,458 requests for endocrinology referrals, it was found that 15% of referrals had insufficient information for evaluation and 71% showed no clinical justification for authorization of referencing. The partial results of the study indicated that the lack of information makes it impossible to clinically regulate these requests. The use of referencing protocols associated with telemedicine tools can assist doctors in primary health care in the clinical management and make access to specialized services more equitable and timely.

Friendship IAP, Maryland. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

DTIC Science & Technology

1982-05-04

USAFETAC PSYCHROMETRIC SUMMARY’k A1 dEATHER SERVXCE/MAC STATIO STATION 041A vt LS MONT. PAGE I1 - i(Fl WI~III~i~i1IIi ET SUL& TESIPEXATUnE DEPRESION (F...WEATHER SERVICE/MAC L2AD0. FRIENDOSHIP IAP MD 74-S1orf STATION STATION *464 VtAAS M. PAGE 2 -nn-nn Th.~.WIT SUL$ TEMPERATURE DEPRESION (F) ITOTAL TOTAL () 0...Alte WEAT.4ER SERVICE/IAC 776ff F7hnSHTP Ti A mn-t ER STAION NWA YA PAGE 1 WE? BULB TEMPERATURE DEPRESION (F) TOTAL 1 TOTAL (I 0 1.2 j3-43. 7. 9. . 1o 1

Integrated evaluation of the geology, aerogammaspectrometry and aeromagnetometry of the Sul-Riograndense Shield, southernmost Brazil.

PubMed

Hartmann, Léo A; Lopes, William R; Savian, Jairo F

2016-03-01

An integrated evaluation of geology, aerogammaspectrometry and aeromagnetometry of the Sul-Riogran-dense Shield is permitted by the advanced stage of understanding of the geology and geochronology of the southern Brazilian Shield and a 2010 airborne geophysical survey. Gamma rays are registered from the rocks near the surface and thus describe the distribution of major units in the shield, such as the Pelotas batholith, the juvenile São Gabriel terrane, the granulite-amphibolite facies Taquarembó terrane and the numerous granite intrusions in the foreland. Major structures are also observed, e.g., the Dorsal de Canguçu shear. Magnetic signals register near surface crustal compositions (analytic signal) and total crust composition (total magnetic signal), so their variation as measured indicates either shallow or whole crustal structures. The Caçapava shear is outstanding on the images as is the magnetic low along the N-S central portion of the shield. These integrated observations lead to the deepening of the understanding of the largest and even detailed structures of the Sul-Riograndense Shield, some to be correlated to field geology in future studies. Most significant is the presence of different provinces and their limits depending on the method used for data acquisition - geology, aerogammaspectrometry or aeromagnetometry.

Persistence of antibiotic resistance genes and bacterial community changes in drinking water treatment system: From drinking water source to tap water.

PubMed

Su, Hao-Chang; Liu, You-Sheng; Pan, Chang-Gui; Chen, Jun; He, Liang-Ying; Ying, Guang-Guo

2018-03-01

As emerging contaminants, antibiotic resistance genes (ARGs) have become a public concern. This study aimed to investigate the occurrence and diversity of ARGs, and variation in the composition of bacterial communities in source water, drinking water treatment plants, and tap water in the Pearl River Delta region, South China. Various ARGs were present in the different types of water. Among the 27 target ARGs, floR and sul1 dominated in source water from three large rivers in the region. Pearson correlation analysis suggested that sul1, sul2, floR, and cmlA could be potential indicators for ARGs in water samples. The total abundance of the detected ARGs in tap water was much lower than that in source water. Sand filtration and sedimentation in drinking water treatment plants could effectively remove ARGs; in contrast, granular activated carbon filtration increased the abundance of ARGs. It was found that Pseudomonas may be involved in the proliferation and dissemination of ARGs in the studied drinking water treatment system. Bacteria and ARGs were still present in tap water after treatment, though they were significantly reduced. More research is needed to optimize the water treatment process for ARG removal. Copyright © 2017 Elsevier B.V. All rights reserved.

[Telling a history ... the creation process of the Brazilian Nurses Association-Rio Grande do Sul Chapter].

PubMed

Mancia, J R; Burlamaque, C S

2001-01-01

This work is a description of the most relevant facts of the branch of the Brazilian Association of Nursing (ABEn) in Rio Grande do Sul federal state, along its fifty years of work. The data was collected in primary sources such as records of the association, newspaper articles, congress minutes, reports from ABEn's members, and pictures. The study contextualizes nursing in the period, which precedes the creation of this branch. It presents biographical aspects of the first directors of the association. Reports the trajectory of this branch, focusing on its struggle to legitimate the nursing profession as a profession that demands higher education and to include it in the career plans of public and private organizations. Describes the efforts of the association to meet the demands of the professional and educational legislation. Presents the work done to strengthen ABEn-Rio Grande do Sul through the organization of campaigns, scientific events and the event Semana da Enfermagem (congress of nursing). It also acknowledges the decisive role of ABEn-RS in the creation and consolidation of the Syndicate of Nursing and of the Federal/Regional Board of Nursing. Finally the study demonstrates that, although it defends specific professional interests, it also has a profound commitment with the quality of the health service provided in Brazil.

[Factors related to the quality of life of the elderly in programs for senior citizens].

PubMed

Dawalibi, Nathaly Wehbe; Goulart, Rita Maria Monteiro; Prearo, Leandro Campi

2014-08-01

Population aging is a social phenomenon that demands the attention of health professionals. This article seeks to analyze the influence of possible intervening factors on the quality of life of the elderly. It was a cross-sectional, quantitative, descriptive and exploratory study, in which 182 elderly of both genders participated. They were aged ≥ 60 years and were enrolled in programs for senior citizens from three universities located in the cities of Sao Paulo, Sao Caetano do Sul and Mogi das Cruzes. To evaluate the subjective perception of quality of life, the World Health Organization Quality of Life questionnaire (WHOQOL-Bref) was used. Multiple linear regression analysis was performed on the dependent and independent variables. A significance level of 5% was adopted. The results showed that the absence of disease positively influenced the quality of life on the four domains evaluated. In the environmental domain, full tertiary education, old age, the absence of disease and being a resident in Sao Caetano do Sul positively influenced the quality of life of the elderly. The factors associated with better quality of life were: full tertiary education, old age, the absence of disease and being a resident in Sao Caetano do Sul, in that order of importance.

Trypanosoma cruzi strains from triatomine collected in Bahia and Rio Grande do Sul, Brazil

PubMed Central

Ribeiro, Aline Rimoldi; Mendonça, Vagner José; Alves, Renata Tomé; Martinez, Isabel; de Araújo, Renato Freitas; Mello, Fernanda; da Rosa, João Aristeu

2014-01-01

OBJECTIVE Collection of triatomines in domestic, peridomestic and sylvatic environments in states of Bahia and Rio Grande do Sul, Northeastern and Southern Brazil respectively, and isolation of Trypanosoma cruzi strains. METHODS First, the captured triatomines were identified using insect identification keys, then their intestinal content was examined by abdominal compression, and the samples containing trypanosomatid forms were inoculated in LIT medium and Swiss mice. RESULTS Six triatomine species were collected in cities in Bahia, namely Panstrongylus geniculatus (01), Triatoma melanocephala (11), T. lenti (94), T. pseudomaculata (02), T. sherlocki (26) and T. sordida (460), and two in cities in Rio Grande do Sul, namely T. circummaculata (11) and T. rubrovaria (115). Out of the specimens examined, T. cruzi was isolated from 28 triatomine divided into four different species: T. melanocephala (one), T. lenti (one), T. rubrovaria (16) and T. sordida (10). Their index of natural infection by T. cruzi was 6.4%. CONCLUSIONS The isolation of T. cruzi strains from triatomines found in domestic and peridomestic areas shows the potential risk of transmission of Chagas disease in the studied cities. The maintenance of those T. cruzi strains in laboratory is intended to promote studies that facilitate the understanding of the parasite-vector-host relationship. PMID:24897051

Social representations of the health care of the Mbyá-Guarani indigenous population by health workers 1

PubMed Central

Falkenberg, Mirian Benites; Shimizu, Helena Eri; Bermudez, Ximena Pamela Díaz

2017-01-01

ABSTRACT Objective: to analyze the social representations of health care of the Mbyá-Guarani ethnic group by multidisciplinary teams from the Special Indigenous Health District in the south coast of Rio Grande do Sul state (Distrito Sanitário Especial Indígena Litoral Sul do Rio Grande do Sul), Brazil. Method: a qualitative method based on the theory of social representations was used. Data were collected via semi-structured interviews with 20 health workers and by participant observation. The interviews were analyzed with ALCESTE software, which conducts a lexical content analysis using quantitative techniques for the treatment of textual data. Results: there were disagreements in the health care concepts and practices between traditional medicine and biomedicine; however, some progress has been achieved in the area of intermedicality. The ethnic boundaries established between health workers and indigenous peoples based on their representations of culture and family, together with the lack of infrastructure and organization of health actions, are perceived as factors that hinder health care in an intercultural context. Conclusion: a new basis for the process of indigenous health care needs to be established by understanding the needs identified and by agreement among individuals, groups, and health professionals via intercultural exchange. PMID:28177056

[Thermal requirements and estimate of the annual number of generations of Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) on strawberry crop].

PubMed

Nondillo, Aline; Redaelli, Luiza R; Botton, Marcos; Pinent, Silvia M J; Gitz, Rogério

2008-01-01

Frankliniella occidentalis (Pergande) is one of the major strawberry pests in southern Brazil. The insect causes russeting and wither in flowers and fruits reducing commercial value. In this work, the thermal requirements of the eggs, larvae and pupae of F. occidentalis were estimated. Thrips development was studied in folioles of strawberry plants at six constant temperatures (16, 19, 22, 25, 28 and 31 degrees C) in controlled conditions (70 +/- 10% R.H. and 12:12 L:D). The number of annual generations of F. occidentalis was estimated for six strawberry production regions of Rio Grande do Sul State based on its thermal requirements. Developmental time of each F. occidentalis stages was proportional to the temperature increase. The best development rate was obtained when insects were reared at 25 masculineC and 28 masculineC. The lower threshold and the thermal requirements for the egg to adult stage were 9.9 masculineC and 211.9 degree-days, respectively. Considering the thermal requirements of F. occidentalis, 10.7, 12.6, 13.1, 13.6, 16.5 and 17.9 generations/year were estimated, respectively, for Vacaria, Caxias do Sul, Farroupilha, Pelotas, Porto Alegre and Taquari producing regions located in Rio Grande do Sul State, Brazil.

Antibiotic resistance phenotypes and virulence-associated genes in Escherichia coli isolated from animals and animal food products in Tunisia.

PubMed

Badi, Souhir; Cremonesi, Paola; Abbassi, Mohamed Salah; Ibrahim, Chourouk; Snoussi, Majdi; Bignoli, Giulia; Luini, Mario; Castiglioni, Bianca; Hassen, Abdennaceur

2018-05-01

Livestock and food products of animal origin constitute important reservoirs of intestinal and extraintestinal pathogenic Escherichia coli including antibiotic-resistant E. coli isolates. To assess potential risks to public health related to E. coli strains of animal origin in Tunisia, 65 E. coli isolates recovered from healthy animals and food products of animal origin were studied. Antimicrobial susceptibility was determined according to CLSI guidelines and genes encoding antibiotic resistance as well as virulence factors were investigated by PCR. High rates of antibiotic resistance were observed to kanamycin (78.4%), gentamicin (75.3%) and streptomycin (75.3%, encoded by strA-strB (7 isolates)), amoxicillin (64.6%), amoxicillin/clavulanic acid (60%), tetracycline (44.6%; tetA (8 isolates) and tetB (7 isolates)), nalidixic acid (27.6%, qnrS (3 isolates), qnrB (2 isolates) and qnrA (one isolate)) and sulfonamides (36.9%; sul1 (1 isolate), sul2 (4 isolates), and sul3 (1 isolate)). Virulotypes classified some isolates as STEC (3%), MNEC (1.5%) and atypical EPEC (1.5%). This study demonstrated high rates of antimicrobial resistance and the presence of some pathogenic pathovars from animal origins that are a cause of concern for public health.

Antimicrobial Resistance and Resistance Genes in Aerobic Bacteria Isolated from Pork at Slaughter.

PubMed

Li, Lili; Heidemann Olsen, Rikke; Ye, Lei; Yan, He; Nie, Qing; Meng, Hecheng; Shi, Lei

2016-04-01

The aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the People's Republic of China. The organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). High levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to nitrofurantoin, cefotaxime, gentamicin, ciprofloxacin, and chloramphenicol (7.8 to 29.2%). Across species, genes conferring antimicrobial resistance were observed with the following frequencies: blaTEM, 40.7%; blaCMY-2, 15.2%; blaCTX-M, 11.5%; sul2, 27.2%; sul1, 14.4%; tet(A), 5.4%; tet(L), 5.4%; tet(M), 5.0%; tet(E), 3.7%; tet(C), 3.3%; tet(S), 2.5%; and tet(K), 0.8%. Various antimicrobial resistance genes were found in new carriers: blaTEM in Lactococcus garvieae, Myroides odoratimimus, Aeromonas hydrophila, Staphylococcus sciuri, Raoultella terrigena, Macrococcus caseolyticus, Acinetobacter ursingii, Sphingobacterium sp., and Oceanobacillus sp.; blaCMY-2 in Lactococcus lactis, Klebsiella oxytoca, Serratia marcescens, Acinetobacter baumannii, and Myroides phaeus; tet(L) in M. caseolyticus; sul1 in Vibrio cincinnatiensis; sul2 in Acinetobacter bereziniae, Acinetobacter johnsonii, and V. cincinnatiensis; and the class 1 integron and gene cassette aadA2 in V. cincinnatiensis. Approximately 6.6% of isolates contained class 1 integrons, and one isolate harbored class 2 integrons. Plasmid associated intI1 and androgen receptor- encoding genes were transferred into Escherichia coli J53 and E. coli DH5α by conjugation and transformation experiments, respectively. Our study highlights the importance of aerobic bacteria from pork as reservoirs for antimicrobial resistance genes and mobile genetic elements that can readily be transferred intra- and interspecies.

HOSPITALIZATIONS FOR CHOLECYSTITIS AND CHOLELITHIASIS IN THE STATE OF RIO GRANDE DO SUL, BRAZIL

PubMed Central

NUNES, Emeline Caldana; ROSA, Roger dos Santos; BORDIN, Ronaldo

2016-01-01

ABSTRACT Background: The cholelithiasis is disease of surgical resolution with about 60,000 hospitalizations per year in the Sistema Único de Saúde (SUS - Brazilian National Health System) of the Rio Grande do Sul state. Aim: To describe the profile of hospitalizations for cholecystitis and cholelithiasis performed by the SUS of Rio Grande do Sul state, 2011-2013. Methods: Hospital Information System data from the National Health System through morbidity list for cholelithiasis and cholecystitis (ICD-10 K80-K81). Variables studied were sex, age, number of hospitalizations and approved Hospitalization Authorizations (AIH), total amount and value of hospital services generated, days and average length of stay, mortality, mortality and case fatality ratio, from health regions of the Rio Grande do Sul. Results: During 2011-2013 there were 60,517 hospitalizations for cholecystitis and cholelithiasis, representing 18.86 hospitalizations per 10,000 inhabitants/year, most often in the age group from 60 to 69 years (41.34 admissions per 10,000 inhabitants/year) and female (27.72 hospitalizations per 10,000 inhabitants/year). The fatality rate presented an inverse characteristic: 13.52 deaths per 1,000 admissions/year for males, compared with 7.12 deaths per 1,000 admissions/year in females. The state had an average total amount spent and value of hospital services of R$ 16,244,050.60 and R$ 10,890,461.31, respectively. The health region "Capital/Gravataí Valley" exhibit the highest total expenditure and hospital services, and the largest number of deaths, and average length of stay. Conclusion: The hospitalization and lethality coefficients, the deaths, the length of stay and spending related to admissions increased from 50 years old. Females had a higher frequency and higher values ​​spent on hospitalization, while the male higher coefficient of mortality and mean hospital stay. PMID:27438030

Occurrence of antibiotics and antibiotic resistance genes in a sewage treatment plant and its effluent-receiving river.

PubMed

Xu, Jian; Xu, Yan; Wang, Hongmei; Guo, Changsheng; Qiu, Huiyun; He, Yan; Zhang, Yuan; Li, Xiaochen; Meng, Wei

2015-01-01

The extensive use of antibiotics has caused the contamination of both antibiotics and antibiotic resistance genes (ARGs) in the environment. In this study, the abundance and distribution of antibiotics and ARGs from a sewage treatment plant (STP) and its effluent-receiving river in Beijing China were characterized. Three classes of antibiotics including tetracycline, sulfonamide and quinolone were quantified by LC-MS/MS. In the secondary effluent they were detected at 195, 2001 and 3866 ng L(-1), respectively, which were higher than in the receiving river water. A total of 13 ARGs (6 tet genes: tetA, tetB, tetE, tetW, tetM and tetZ, 3 sulfonamide genes: sul1, sul2 and sul3, and 4 quinolone genes: gryA, parC, qnrC and qnrD) were determined by quantitative PCR. For all ARGs, sulfonamide resistance genes were present at relatively high concentrations in all samples, with the highest ARG concentration above 10(-1). ARGs remained relatively stable along each sewage treatment process. The abundances of detected ARGs from the STP were also higher than its receiving river. Bivariate correlation analysis showed that relative tet gene copies (tetB/16S-rRNA and tetW/16S-rRNA) were strongly correlated with the concentrations of tetracycline residues (r(2)>0.8, p<0.05), while no significant correlations occurred between sulfonamides and sul genes. A negative correlation between the relative abundance of quinolone resistance gene (qnrC/16S-rRNA) and the concentrations of enrofloxacin (ENR) was also determined. The difference of ARGs levels in the raw influent and secondary effluent suggested that the STP treatment process may induce to increase the abundance of resistance genes. The results showed that the sewage was an important repository of the resistance genes, which need to be effectively treated before discharge into the natural water body. Copyright © 2014 Elsevier Ltd. All rights reserved.

The importance of integrons for development and propagation of resistance in Shigella: the case of Latin America.

PubMed

Barrantes, Kenia; Achí, Rosario

In Latin America, the disease burden of shigellosis is found to coexist with the rapid and rampant spread of resistance to commonly used antibiotics. The molecular basis of antibiotic resistance lies within genetic elements such as plasmids, transposons, integrons, genomic islands, etc., which are found in the bacterial genome. Integrons are known to acquire, exchange, and express genes within gene cassettes and it is hypothesized that they play a significant role in the transmission of multidrug resistance genes in several Gram-negative bacteria including Shigella. A few studies have described antibiotic resistance genes and integrons among multidrug resistant Shigella isolates found in Latin America. For example, in Brazil, Bolivia, Chile, Costa Rica and Peru, class 1 and class 2 integrons have been detected among multidrug resistant strains of Shigella; this phenomenon is more frequently observed in S. flexneri isolates that are resistant to trimethoprim, sulfamethoxazole, streptomycin, ampicillin, chloramphenicol, and tetracycline. The gene cassette sul2, which is frequently detected in Shigella strains resistant to the sulfonamides, suggests that the sulfonamide-resistant phenotype can be explained by the presence of the sul2 genes independent of the integron class detected. It is to be noted that sul3 was negative in all isolates analyzed in these studies. The high frequency of sulfonamide (as encoded by sul2) and trimethoprim resistance is likely to be a result of the recurrent use of trimethoprim sulfamethoxazole as a popular regimen for the treatment of shigellosis. The observed resistance profiles of Shigella strains confirm that ampicillin and trimethoprim-sulfamethoxazole are ineffective as therapeutic options. In-depth information regarding antibiotic resistance mechanism in this pathogen is needed in order to develop suitable intervention strategies. There is a pressing need for regional and local antimicrobial resistance profiling of Shigella to be included as a part of the public health strategy. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

Dissemination of antibiotic resistance genes in representative broiler feedlots environments: identification of indicator ARGs and correlations with environmental variables.

PubMed

He, Liang-Ying; Liu, You-Sheng; Su, Hao-Chang; Zhao, Jian-Liang; Liu, Shuang-Shuang; Chen, Jun; Liu, Wang-Rong; Ying, Guang-Guo

2014-11-18

Livestock operations are known to harbor elevated levels of antibiotic resistance genes (ARGs) that may pose a threat to public health. Broiler feedlots may represent an important source of ARGs in the environment. However, the prevalence and dissemination mechanisms of various types of ARGs in the environment of broiler feedlots have not previously been identified. We examined the occurrence, abundance and variation of ARGs conferring resistance to chloramphenicols, sulfonamides and tetracyclines in the environments of two representative types of broiler feedlots (free range and indoor) by quantitative PCR, and assessed their dissemination mechanisms. The results showed the prevalence of various types of ARGs in the environmental samples of the broiler feedlots including manure/litter, soil, sediment, and water samples, with the first report of five chloramphenicol resistance genes (cmlA, floR, fexA, cfr, and fexB) in broiler feedlots. Overall, chloramphenicol resistance genes and sulfonamides sul genes were more abundant than tetracyclines tet genes. The ARG abundances in the samples from indoor boiler feedlots were generally different to the free range feedlots, suggesting the importance of feeding operations in ARG dissemination. Pearson correlation analysis showed significant correlations between ARGs and mobile genetic element genes (int1 and int2), and between the different classes of ARGs themselves, revealing the roles of horizontal gene transfer and coselection for ARG dissemination in the environment. Further regression analysis revealed that fexA, sul1 and tetW could be reliable indicator genes to surrogate anthropogenic sources of ARGs in boiler feedlots (correlations of fexA, sul1 and tetW to all ARGs: R = 0.95, 0.96 and 0.86, p < 0.01). Meanwhile, significant correlations were also identified between indicator ARGs and their corresponding antibiotics. In addition, some ARGs were significantly correlated with typical metals (e.g., Cu, Zn, and As with fexA, fexB, cfr, sul1, tetW, tetO, tetS: R = 0.52-0.71) and some environmental parameters (e.g., TOC, TN, TP, NH3-N with fexA, fexB, cfr, sul1, tetW, tetO, tetQ, tetS: R = 0.53-0.87) (p < 0.01). Further redundancy analysis demonstrated that the distribution and transportation of ARGs from the boiler feedlots to the receiving environments were correlated with environmental variables. The findings highlight the contribution of some chemicals such as antibiotics and metals to the development of ARGs in broiler feedlots environments; and the observed ARG dissemination mechanism in the broiler feedlots facilitates the development of effective mitigation measures.

Tephra layers from Holocene lake sediments of the Sulmona Basin, central Italy: implications for volcanic activity in Peninsular Italy and tephrostratigraphy in the central Mediterranean area

NASA Astrophysics Data System (ADS)

Giaccio, B.; Messina, P.; Sposato, A.; Voltaggio, M.; Zanchetta, G.; Galadini, F.; Gori, S.; Santacroce, R.

2009-12-01

We present a new tephrostratigraphic record from the Holocene lake sediments of the Sulmona basin, central Italy. The Holocene succession is represented by whitish calcareous mud that is divided into two units, SUL2 (ca 32 m thick) and SUL1 (ca 8 m thick), for a total thickness of ca 40 m. These units correspond to the youngest two out of six sedimentary cycles recognised in the Sulmona basin that are related to the lake sedimentation since the Middle Pleistocene. Height concordant U series age determinations and additional chronological data constrain the whole Holocene succession to between ca 8000 and 1000 yrs BP. This includes a sedimentary hiatus that separates the SUL2 and SUL1 units, which is roughly dated between <2800 and ca 2000 yrs BP. A total of 31 and 6 tephra layers were identified within the SUL2 and SUL1 units, respectively. However, only 28 tephra layers yielded fresh micro-pumices or glass shards suitable for chemical analyses using a microprobe wavelength dispersive spectrometer. Chronological and compositional constraints suggest that 27 ash layers probably derive from the Mt. Somma-Vesuvius Holocene volcanic activity, and one to the Ischia Island eruption of the Cannavale tephra (2920 ± 450 cal yrs BP). The 27 ash layers compatible with Mt. Somma-Vesuvius activity are clustered in three different time intervals: from ca 2000 to >1000; from 3600 to 3100; and from 7600 to 4700 yrs BP. The first, youngest cluster, comprises six layers and correlates with the intense explosive activity of Mt. Somma-Vesuvius that occurred after the prominent AD 79 Pompeii eruption, but only the near-Plinian event of AD 472 has been tentatively recognised. The intermediate cluster (3600-3100 yrs BP) starts with tephra that chemically and chronologically matches the products from the "Pomici di Avellino" eruption (ca 3800 ± 200 yrs BP). This is followed by eight further layers, where the glasses exhibit chemical features that are similar in composition to the products from the so-called "Protohistoric" or AP eruptions; however, only the distal equivalents of three AP events (AP3, AP4 and AP6) are tentatively designated. Finally, the early cluster (7600-4700 yrs BP) comprises 12 layers that contain evidence of a surprising, previously unrecognised, activity of the Mt. Somma-Vesuvius volcano during its supposed period of quiescence, between the major Plinian "Pomici di Mercato" (ca 9000 yrs BP) and "Pomici di Avellino" eruptions. Alternatively, since at present there is no evidence of a similar significant activity in the proximal area of this well-known volcano, a hitherto unknown origin of these tephras cannot be role out. The results of the present study provide new data that enrich our previous knowledge of the Holocene tephrostratigraphy and tephrochronology in central Italy, and a new model for the recent explosive activity of the Peninsular Italy volcanoes and the dispersal of the related pyroclastic deposits.

Profile and scientific production of Brazilian National Council of Technological and Scientific Development researchers in Pediatrics.

PubMed

Oliveira, Maria Christina L; Martelli, Daniella Reis B; Pinheiro, Sergio Veloso; Miranda, Debora Marques; Quirino, Isabel Gomes; Leite, Barbara Gusmão L; Colosimo, Enrico Antonio; e Silva, Ana Cristina S; Martelli-Júnior, Hercílio; Oliveira, Eduardo Araujo

2013-09-01

To evaluate the profile and the scientific production of researchers in Pediatrics with scholarship from the National Counsel of Technological and Scientific Development. The Lattes curricula of 34 researchers in Pediatrics with active scholarships, from 2006 to 2008 were included in the analysis. The variables of interest were: gender, affiliation, time since PHD, tutoring of undergraduate students, mentorship of masters and doctors, scientific production and the papers' impact. In a total of 411 researchers in Medicine, 34 (8%) belonged to Pediatrics. Males (77%) and scholars in the category 2 of productivity (62%) prevailed. Three states of Brazil were responsible for approximately 90% of the researchers: São Paulo (53%), Minas Gerais (21%), and Rio Grande do Sul (15%). During their academic career, the Pediatrics researchers have published 3,122 articles with a median of 89 articles per researcher (interquartile range - IQ=51-119). Of the total, 40 and 59% articles were indexed in the Web of Science and Scopus databases, respectively. The Pediatrics researchers have published papers in 599 journals with a median impact factor of 2.35 (IQ=1.37-3.73) for the 323 indexed journals. The Pediatrics researchers have a relevant scientific output from the quantity point of the view, but there is a need to improve quality.

[Establishing ward-independent, intensive treatment concept in a psychiatric hospital : A model project within the new German remuneration system].

PubMed

Noeker, M; Juckel, G

2017-03-01

Model projects according to § 64b of the Social Code V in the context of the new remuneration system in psychiatry and psychosomatics, offer great possibilities to improve the treatment of people with mental illnesses. This article presents the model project of the University Hospital Bochum, which is essentially characterized by improved transition through the internal hospital sections so that patients can be quickly transferred from inpatient and daycare sections to high frequency outpatient sections with ward-independent therapies (SUL), including outreach home treatment. The SUL is also intended to facilitate preadmission crises, to significantly reduce duration of inpatient treatment and to maximize post-inpatient continuity of treatment.

Macroergonomic intervention for work design improvement and raw materials waste reduction in a small footwear components company in Rio Grande do Sul-Brazil.

PubMed

Cornelli, Renata; Guimarães, Lia Buarque de Macedo

2012-01-01

This article presents a macroergonomic intervention carried out in a small footwear components company located in the state of Rio Grande do Sul, Brazil. The company's demand was related to the waste of the expensive raw-material (thermoplastic polyurethane or TPU) used to manufacture the components (high heels pegs). According to the managerial staff, the waste was workers responsibility due to the craft characteristic of the process. A participative method was used to evaluate the problems, propose and implement solutions, as well as evaluate their impact on the workers and the Company. Improvements in the work conditions resulted in increase of workers' satisfaction with the work and in 31.5% waste reduction.

Helminth parasites of Leptodactylus podicipinus (Anura: Leptodactylidae) from south-eastern Pantanal, State of Mato Grosso do Sul, Brazil.

PubMed

Campião, K M; da Silva, R J; Ferreira, V L

2009-12-01

Forty-three specimens of Leptodactylus podicipinus (Anura: Leptodactylidae) were collected in the south-eastern Pantanal, municipality of Corumbá, State of Mato Grosso do Sul, Brazil in February and July 2007, and examined for endoparasites. Forty (93%) specimens were infected with at least one helminth species. The predominant parasites were nematodes (Aplectana sp., Cosmocerca podicipinus, Oswaldocruzia lopesi, Physalopteroides venancioi, Rhabdias sp.), but the trematode Catadiscus propinquus also showed high prevalence. The trematodes Infidum infidum and Travtrema stenocotyle were also found, but in only one specimen. Adult frogs showed higher parasite diversity than subadults. Leptodactylus podicipinus was preferentially infected by direct life-cycle parasites and was reported as a new host record for seven helminth species.

The tadpole of Hypsiboas guentheri (Boulenger) (Anura: Hylidae).

PubMed

Almeida-Silva, Diego; Neto, Antonio Mollo; Mendes, Humberto Fonseca; Verdade, Vanessa Kruth

2016-10-28

Hypsiboas guentheri is a species of treefrog that inhabits the surrounding vegetation of temporary and permanent ponds at the coastal region of Southern Brazil, in the States of Santa Catarina and Rio Grande do Sul (Langone 1997; Frost 2016). It belongs to the H. pulchellus species group (Faivovich et al. 2004, Duellman et al. 2016), this group contains 39 species (Frost 2016), but formal descriptions of tadpoles are missing for 15 of them (Kolenc et al. 2008; Pinheiro et al., 2016). Herein we describe tadpoles of H. guentheri collected in February 2014 in the accumulated water on an underpass for wildlife crossing at the RS-486 highway (29º30'44.8" S 50º06'29.9" W), Itati municipality, Rio Grande do Sul State, Brazil.

[Workers with signs of smallpox in the collection of Regional Office of Labor, Rio Grande do Sul, 1933-1944].

PubMed

Lopes, Aristeu Elisandro Machado

2016-01-01

Work Register Booklet was created in Brazil in 1932. Soon, Regional Labor Inspectorates emerged - after renamed as Regional Office of Labor. In Rio Grande do Sul, this office was settled in 1933 in Porto Alegre. Procedures for making this booklet consisted of filling a professional qualification form with workers' personal and professional information. One of the fields consisted of requester's distinguishing signs, like visible marks and lack of limbs. The purpose of this article is to analyse the presence of one of these distinguishing signs. We use 3x4cm photos of workers who presented smallpox signs, as well as other information written in the fields of their forms.

Use of LANDSAT images to study cerrado vegetation. [Mato Grosso Sul, Brazil

NASA Technical Reports Server (NTRS)

Parada, N. D. J. (Principal Investigator); Filho, P. H.

1982-01-01

Channel 5 and 7 LANDSAT imagery at the scale of 1:250,000 made during passes in the dry and rainy seasons were used to select the optimal season for cerrado characterization in Mato Grosso do Sul State. The study area is located around the cities of Campo Grande and Tres Lagoas, a region being used for reforestation and rangeland activities. Imagery acquired during the dry season permitted a good discrimination between "cerrado" (woodsy pasture) vegetation and reforestation. In relation to the altered areas, only the recently modified area presented good discrimination of cerrado vegetation. Imagery of the rainy season did not provide a reasonable separation between cerrado and reforestation areas but the altered area could be easily discriminated.

A new species of tree crickets Oecanthus (Orthoptera, Gryllidae, Oecanthinae) in tobacco plantation from Southern Brazil, with body color variation.

PubMed

Milach, Elisa Machado; Martins, Luciano De P; Da Costa, Maria Kátia Matiotti; Gottschalk, Marco Silva; De Oliveira, Gabriel Lobregat; Redü, Darlan Rutz; Neutzling, Alexandre Schneid; Dornelles, José Eduardo Figueiredo; Vasconcellos, Lucas Azevedo; Zefa, Edison

2015-09-15

We provide herein a description of a new species of Oecanthus collected from the tobacco plantation in southern Brazil, municipality of São Lourenço do Sul, State of Rio Grande do Sul. Description focused metanotal gland features, phallic sclerites, and calling song. A large sampling of individuals was distributed into four groups according to body and appendages color and dotted. We also rank all kind of marks present in the scape and pedicel. We compare linear morphometric variables of the metanotal glands and tegmina, as well as calling song parameters between groups in order do define all of them as the same species. Photographs and measurements are provided.

Moral sensitivity in Primary Health Care nurses.

PubMed

Nora, Carlise Rigon Dalla; Zoboli, Elma Lourdes Campos Pavone; Vieira, Margarida M

2017-04-01

to characterize the profile and describe the moral sensitivity of primary health care nurses. this is a quantitative, transversal, exploratory, descriptive study. The data were collected through the Moral Sensitivity Questionnaire translated and adapted to Brazil. 100 primary health care nurses participated, from Rio Grande do Sul, Brazil. The data collection took place during the months of March and July 2016, in an online form. The analysis of the data occurred through descriptive statistical analysis. the nurses had an average moral sensitivity of 4.5 (out of 7). The dimensions with the greatest moral sensitivity were: interpersonal orientation, professional knowledge, moral conflict and moral meaning. the nurses of Rio Grande do Sul have a moderate moral sensitivity, which may contribute to a lower quality in Primary Health Care.

Association between components of family caregivers' sense of burden and types of paid care services provided in Japan.

PubMed

Nakagawa, Yoshinori; Nasu, Seigo

2011-08-01

The aim was to identify significant relationships between the 21 components of caregivers' sense of burden in the Zarit Burden Interview and specific paid care services provided in Japan's long-term care insurance system. We defined a service utilization level (SUL) that represents the amount of care services that caregivers were consuming. We calculated the ratios of people, among those with the same SUL value, whose sense of burden was weaker than a specific level. Finally, we conducted regression analysis and checked how this ratio varied compared to the change in SUL values. For 12 among 22 components, the use of paid care services in general were significantly and linearly related with a smaller number of people having the strongest sense of burden. Several pairs of burden components and care service types were identified indicating that the type of care services effectively alleviated that burden component. (1) Paid care services do relieve caregivers' sense of burden. (2) Measures to increase the ratio of people with the weakest sense of burden by encouraging the use of care services do not necessarily match those that decrease the ratio of people feeling the heaviest burden. (3) Policies that encourage caregivers to use more care services can be more effective if policy makers know which type of care service is related with a burden component.

Analysis of suicide mortality in Brazil: spatial distribution and socioeconomic context.

PubMed

Dantas, Ana P; Azevedo, Ulicélia N de; Nunes, Aryelly D; Amador, Ana E; Marques, Marilane V; Barbosa, Isabelle R

2018-01-01

To perform a spatial analysis of suicide mortality and its correlation with socioeconomic indicators in Brazilian municipalities. This is an ecological study with Brazilian municipalities as a unit of analysis. Data on deaths from suicide and contextual variables were analyzed. The spatial distribution, intensity and significance of the clusters were analyzed with the global Moran index, MoranMap and local indicators of spatial association (LISA), seeking to identify patterns through geostatistical analysis. A total of 50,664 deaths from suicide were registered in Brazil between 2010 and 2014. The average suicide mortality rate in Brazil was 5.23/100,000 population. The Brazilian municipalities presenting the highest rates were Taipas do Tocantins, state of Tocantins (79.68 deaths per 100,000 population), Itaporã, state of Mato Grosso do Sul (75.15 deaths per 100,000 population), Mampituba, state of Rio Grande do Sul (52.98 deaths per 100,000 population), Paranhos, state of Mato Grosso do Sul (52.41 deaths per 100,000 population), and Monjolos, state of Minas Gerais (52.08 deaths per 100,000 population). Although weak spatial autocorrelation was observed for suicide mortality (I = 0.2608), there was a formation of clusters in the South. In the bivariate spatial and classical analysis, no correlation was observed between suicide mortality and contextual variables. Suicide mortality in Brazil presents a weak spatial correlation and low or no spatial relationship with socioeconomic factors.

Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16

PubMed Central

2014-01-01

Background Ovine epididymitis is predominantly associated with Brucella ovis infection. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats (VNTR) analyses (MLVA) have proved to be a powerful tool for epidemiological trace-back studies. Thus, the aim of this study was to evaluate the genetic diversity of Brucella ovis isolates from Rio Grande do Sul State, Brazil, by MLVA16. Findings MLVA16 genotyping identified thirteen distinct genotypes and a Hunter-Gaston diversity index of 0.989 among the fourteen B. ovis genotyped strains. All B. ovis MLVA16 genotypes observed in the present study represented non-previously described profiles. Analyses of the eight conserved loci included in panel 1 (MLVA8) showed three different genotypes, two new and one already described for B. ovis isolates. Among ten B. ovis isolates from same herd only two strains had identical pattern, whereas the four isolates with no epidemiologic information exhibited a single MLVA16 pattern each. Analysis of minimal spanning tree, constructed using the fourteen B. ovis strains typed in this study together with all nineteen B. ovis MLVA16 genotypes available in the MLVAbank 2014, revealed the existence of two clearly distinct major clonal complexes. Conclusions In conclusion, the results of the present study showed a high genetic diversity among B. ovis field isolates from Rio Grande do Sul State, Brazil, by MLVA16. PMID:25015223

Genetic diversity of Brucella ovis isolates from Rio Grande do Sul, Brazil, by MLVA16.

PubMed

Dorneles, Elaine M S; Freire, Guilherme N; Dasso, Maurício G; Poester, Fernando P; Lage, Andrey P

2014-07-12

Ovine epididymitis is predominantly associated with Brucella ovis infection. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats (VNTR) analyses (MLVA) have proved to be a powerful tool for epidemiological trace-back studies. Thus, the aim of this study was to evaluate the genetic diversity of Brucella ovis isolates from Rio Grande do Sul State, Brazil, by MLVA16. MLVA16 genotyping identified thirteen distinct genotypes and a Hunter-Gaston diversity index of 0.989 among the fourteen B. ovis genotyped strains. All B. ovis MLVA16 genotypes observed in the present study represented non-previously described profiles. Analyses of the eight conserved loci included in panel 1 (MLVA8) showed three different genotypes, two new and one already described for B. ovis isolates. Among ten B. ovis isolates from same herd only two strains had identical pattern, whereas the four isolates with no epidemiologic information exhibited a single MLVA16 pattern each. Analysis of minimal spanning tree, constructed using the fourteen B. ovis strains typed in this study together with all nineteen B. ovis MLVA16 genotypes available in the MLVAbank 2014, revealed the existence of two clearly distinct major clonal complexes. In conclusion, the results of the present study showed a high genetic diversity among B. ovis field isolates from Rio Grande do Sul State, Brazil, by MLVA16.

Assessing the risk of bovine fasciolosis using linear regression analysis for the state of Rio Grande do Sul, Brazil.

PubMed

Silva, Ana Elisa Pereira; Freitas, Corina da Costa; Dutra, Luciano Vieira; Molento, Marcelo Beltrão

2016-02-15

Fasciola hepatica is the causative agent of fasciolosis, a disease that triggers a chronic inflammatory process in the liver affecting mainly ruminants and other animals including humans. In Brazil, F. hepatica occurs in larger numbers in the most Southern state of Rio Grande do Sul. The objective of this study was to estimate areas at risk using an eight-year (2002-2010) time series of climatic and environmental variables that best relate to the disease using a linear regression method to municipalities in the state of Rio Grande do Sul. The positivity index of the disease, which is the rate of infected animal per slaughtered animal, was divided into three risk classes: low, medium and high. The accuracy of the known sample classification on the confusion matrix for the low, medium and high rates produced by the estimated model presented values between 39 and 88% depending of the year. The regression analysis showed the importance of the time-based data for the construction of the model, considering the two variables of the previous year of the event (positivity index and maximum temperature). The generated data is important for epidemiological and parasite control studies mainly because F. hepatica is an infection that can last from months to years. Copyright © 2015 Elsevier B.V. All rights reserved.

Analysis of extreme wave events in the southern coast of Brazil

NASA Astrophysics Data System (ADS)

Guimarães, P. V.; Farina, L.; Toldo, E.

2014-06-01

Using the model SWAN, high waves on the Southwestern Atlantic generated by extra-tropical cyclones are simulated from 2000 to 2010 and their impact on the Rio Grande do Sul coast is studied. The modeled waves are compared with buoy data and good agreement is found. The six extreme events in the period which presented significant wave heights above 5 m, on a particular point of interest, are investigated in detail. It is found that the cyclogenetic pattern between the latitudes 31.5 and 34° S, is the most favorable for developing high waves. Hovmöller diagrams for deep water show that the region between the south of Rio Grande do Sul up to latitude 31.5° S is the most energetic during a cyclone's passage, although the event of May 2008 indicate that the location of this region can vary, depending on the cyclone's displacement. On the oher hand, the Hovmöller diagrams for shallow water show that the different shoreface morphologies were responsable for focusing or dissipating the waves' energy; the regions found are in agreement with the observations of erosion and progradation regions. It can be concluded that some of the urban areas of the beaches of Hermenegildo, Cidreira, Pinhal, Tramandaí, Imbé and Torres have been more exposed during the extreme wave events at Rio Grande do Sul coast, and are more vulnerable to this natural hazard.

Behavior of tetracycline and sulfamethazine with corresponding resistance genes from swine wastewater in pilot-scale constructed wetlands.

PubMed

Liu, Lin; Liu, Yu-Hong; Wang, Zhen; Liu, Chao-Xiang; Huang, Xu; Zhu, Ge-Fu

2014-08-15

Four pilot-scale constructed wetlands (free water surface, SF; horizontal subsurface flow, HSF; vertical subsurface flows with different water level, VSF-L and VSF-H) were operated to assess their ability to remove sulfamethazine (SMZ) and tetracycline (TC) from wastewaters, and to investigate the abundance level of corresponding resistance genes (sulI, sulII, tetM, tetW and tetO) in the CWs. The results indicated that CWs could significantly reduce the concentration of antibiotics in wastewater, and the mass removal rate range of SMZ and TC were respectively 11%-95% and 85%-95% in the four systems on the basis of hydraulic equilibrium; further relatively high removal rate was observed in VSF with low water level. Seasonal condition had a significant effect on SMZ removal in the CWs (especially SMZ in SF), but TC removal in VSFs were not considered to have statistically significant differences in winter and summer. At the end period, the relative abundances of target genes in the CWs showed obvious increases compared to initial levels, ranging from 2.98 × 10(-5) to 1.27 × 10(-1) for sul genes and 4.68 × 10(-6) to 1.54 × 10(-1) for tet genes after treatment, and those abundances showed close relation to both characteristic of wastewater and configuration of CWs. Copyright © 2014 Elsevier B.V. All rights reserved.

Social representations of the health care of the Mbyá-Guarani indigenous population by health workers.

PubMed

Falkenberg, Mirian Benites; Shimizu, Helena Eri; Bermudez, Ximena Pamela Díaz

2017-02-06

to analyze the social representations of health care of the Mbyá-Guarani ethnic group by multidisciplinary teams from the Special Indigenous Health District in the south coast of Rio Grande do Sul state (Distrito Sanitário Especial Indígena Litoral Sul do Rio Grande do Sul), Brazil. a qualitative method based on the theory of social representations was used. Data were collected via semi-structured interviews with 20 health workers and by participant observation. The interviews were analyzed with ALCESTE software, which conducts a lexical content analysis using quantitative techniques for the treatment of textual data. there were disagreements in the health care concepts and practices between traditional medicine and biomedicine; however, some progress has been achieved in the area of intermedicality. The ethnic boundaries established between health workers and indigenous peoples based on their representations of culture and family, together with the lack of infrastructure and organization of health actions, are perceived as factors that hinder health care in an intercultural context. a new basis for the process of indigenous health care needs to be established by understanding the needs identified and by agreement among individuals, groups, and health professionals via intercultural exchange. analisar as representações sociais do cuidado em saúde entre trabalhadores que atuam em equipes multidisciplinares no Distrito Sanitário Especial Indígena Litoral Sul do Rio Grande do Sul, junto à etnia Mbyá-Guarani. utilizou-se método qualitativo, fundamentado na teoria das Representações Sociais. Os dados foram coletados por meio de entrevistas semiestruturadas com 20 trabalhadores e da observação participante. As entrevistas analisadas com o software ALCESTE, o qual realiza a análise lexical de conteúdo por meio de técnicas quantitativas de tratamento de dados textuais. verificou-se que existe tensão entre as concepções e práticas de cuidado da medicina tradicional e da biomedicina, mas observam-se alguns avanços na perspectiva da intermedicalidade. Fronteiras étnicas estabelecidas entre os trabalhadores e os indígenas, baseadas em suas representações de cultura e família, da mesma maneira que a falta de infraestrutura e os modos de organização do trabalho em saúde são percebidos como fatores que dificultam o cuidado em contextos de interculturalidade. é preciso estabelecer novas bases para o processo de cuidar em saúde indígena, a partir da compreensão das necessidades representadas, negociadas entre sujeitos individuais e coletivos e os profissionais nas instituições de saúde em um diálogo intercultural de múltiplas vozes. analizar las representaciones sociales de la atención de la salud del grupo étnico Mbyá-Guaraní por equipos multidisciplinarios del Distrito Sanitario Especial Indígena en la costa sur del estado de Rio Grande do Sul (Distrito Sanitário Especial Indígena Litoral Sul do Rio Grande do Sul), Brasil. se utilizó un método cualitativo basado en la teoría de las representaciones sociales. Los datos fueron recogidos a través de entrevistas semi-estructuradas con 20 trabajadores de la salud y por observación de los participantes. Las entrevistas fueron analizadas con el programa ALCESTE, que realiza un análisis de contenido léxico utilizando técnicas cuantitativas para el tratamiento de datos textuales. hubo desacuerdos en los conceptos y prácticas de atención médica entre medicina tradicional y biomedicina; sin embargo, se han logrado algunos avances en el área de la intermedicina. Las barreras étnicas establecidas entre los trabajadores de la salud y los pueblos indígenas basados en sus representaciones de la cultura y la familia, junto con la falta de infraestructura y organización de las acciones de salud, son percibidas como factores que obstaculizan la atención sanitaria en un contexto intercultural. es necesario establecer una nueva base para el proceso de atención de salud indígena mediante la comprensión de las necesidades identificadas y por acuerdo entre individuos, grupos y profesionales de la salud a través del intercambio intercultural.

A toxic cyanobacterial bloom in an urban coastal lake, Rio Grande do Sul state, Southern Brazil

PubMed Central

de Carvalho, Luciana Retz; Pipole, Fernando; Werner, Vera Regina; Laughinghouse IV, Haywood Dail; de Camargo, Antonio Carlos M.; Rangel, Marisa; Konno, Katsuhiro; Sant’ Anna, Célia Leite

2008-01-01

Reports of cyanobacterial blooms developing worldwide have considerably increased, and, in most cases, the predominant toxins are microcystins. The present study reports a cyanobacterial bloom in Lake Violão, Torres, Rio Grande do Sul State, in January 2005. Samples collected on January 13, 2005, were submitted to taxonomical, toxicological, and chemical studies. The taxonomical analysis showed many different species of cyanobacteria, and that Microcystis protocystis and Sphaerocavum cf. brasiliense were dominant. Besides these, Microcystis panniformis, Anabaena oumiana, Cylindrospermopsis raciborskii, and Anabaenopsis elenkinii f. circularis were also present. The toxicity of the bloom was confirmed through intraperitoneal tests in mice, and chemical analyses of bloom extracts showed that the major substance was anabaenopeptin F, followed by anabaenopeptin B, microcystin-LR, and microcystin-RR. PMID:24031304

PER-8, a Novel Extended-Spectrum β-Lactamase PER Variant, from an Acinetobacter baumannii Clinical Isolate in Nepal.

PubMed

Tada, Tatsuya; Shrestha, Shovita; Shimada, Kayo; Ohara, Hiroshi; Sherchand, Jeevan B; Pokhrel, Bharat M; Kirikae, Teruo

2017-03-01

A novel PER-type extended-spectrum β-lactamase, PER-8, was identified in an Acinetobacter baumannii clinical isolate obtained in Nepal. The amino acid sequence of PER-8 has a substitution at position 39 (Gly to Glu) compared with that of PER-7. The k cat / K m ratio of PER-8 for aztreonam was lower than that of PER-7, while the k cat / K m ratio of PER-8 for imipenem was higher than that of PER-7. The genomic environment surrounding bla PER-8 was intI1 bla PSE-1 qacEDI sulI IS CR1-bla PER-8 gts sulI orfX on a 100-kb plasmid. Copyright © 2017 American Society for Microbiology.

Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil.

PubMed

Nõlvak, Hiie; Truu, Marika; Kanger, Kärt; Tampere, Mailiis; Espenberg, Mikk; Loit, Evelin; Raave, Henn; Truu, Jaak

2016-08-15

Soil fertilization with animal manure or its digestate may facilitate an important antibiotic resistance dissemination route from anthropogenic sources to the environment. This study examines the effect of mineral fertilizer (NH4NO3), cattle slurry and cattle slurry digestate amendment on the abundance and proportion dynamics of five antibiotic resistance genes (ARGs) and two classes of integron-integrase genes (intI1 and intI2) in agricultural grassland soil. Fertilization was performed thrice throughout one vegetation period. The targeted ARGs (sul1, tetA, blaCTX-M, blaOXA2 and qnrS) encode resistance to several major antibiotic classes used in veterinary medicine such as sulfonamides, tetracycline, cephalosporins, penicillin and fluoroquinolones, respectively. The non-fertilized grassland soil contained a stable background of tetA, blaCTX-M and sul1 genes. The type of applied fertilizer significantly affected ARGs and integron-integrase genes abundances and proportions in the bacterial community (p<0.001 in both cases), explaining 67.04% of the abundance and 42.95% of the proportion variations in the grassland soil. Both cattle slurry and cattle slurry digestate proved to be considerable sources of ARGs, especially sul1, as well as integron-integrases. Sul1, intI1 and intI2 levels in grassland soil were elevated in response to each organic fertilizer's application event, but this increase was followed by a stage of decrease, suggesting that microbes possessing these genes were predominantly entrained into soil via cattle slurry or its digestate application and had somewhat limited survival potential in a soil environment. However, the abundance of these three target genes did not decrease to a background level by the end of the study period. TetA was most abundant in mineral fertilizer treated soil and blaCTX-M in cattle slurry digestate amended soil. Despite significantly different abundances, the abundance dynamics of bacteria possessing these genes were similar (p<0.05 in all cases) in different treatments and resembled the dynamics of the whole bacterial community abundance in each soil treatment. Copyright © 2016. Published by Elsevier B.V.

Genetic diversity of Mycobacterium tuberculosis isoniazid monoresistant and multidrug-resistant in Rio Grande do Sul, a tuberculosis high-burden state in Brazil.

PubMed

Esteves, Leonardo Souza; Dalla Costa, Elis Regina; Vasconcellos, Sidra Ezidio Gonçalves; Vargas, Andrei; Ferreira Junior, Sérgio Luis Montego; Halon, Maria Laura; Ribeiro, Marta Osorio; Rodenbusch, Rodrigo; Gomes, Harrison Magdinier; Suffys, Philip N; Rossetti, Maria Lucia R

2018-05-01

Tuberculosis (TB) remains a major public health problem in the world and Brazil is among the countries with the highest incidence and prevalence rates, and Rio Grande do Sul, a Brazilian state, occupy a prominent position. Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) further aggravates this scenario, making it more difficult to treat and control the disease. Isoniazid monoresistance (IMR) may increase the risk of progression to MDR-TB and treatment failure. However, most drug resistance molecular tests only focus on detecting rifampicin (RIF) resistance.In the present study, we characterized a total of 63 drug resistant isolates of M. tuberculosis (35 MDR, 26 IMR and two isolates monoresistant to rifampicin [RMR]) of the Rio Grande do Sul state by MIRU-VNTR (24 loci), spoligotyping, presence of RD Rio , fbpC 103 , pks15/1 and sequencing of the katG, rpoB and inhA genes. We observed a higher proportion of the LAM family 30/63 (47.61%). In IMR, mutations were found in the katG gene (98% at codon 315) in 72.5%, and mutations in the promoter region of the inhA gene in 6.25% of the isolates. In MDR-TB and RMR-TB isolates, 92.1% had mutations in the rpoB gene (57% at codon 531). The presence of a 12 bp insertion between codons 516 and 517 of the rpoB gene in MDR-TB isolates was found in five isolates. In conclusion, we observed that the highest frequency of IMR-TB and MDR-TB strains belong to the LAM and Haarlem genotypes in Rio Grande do Sul state. A significant number of isolates previously characterized as Mycobacterium pinnipedi2 through spoligotyping were found to belong to the M. tuberculosis LAM family. This was responsible for a number of significant cases and the molecular profile of this strain and the pattern of mutations related to drug resistance were analyzed. These findings may contribute to a better understanding about the spread of M. tuberculosis resistant in southern of Brazil. Copyright © 2018 Elsevier Ltd. All rights reserved.

Accumulation of Pharmaceuticals, Enterococcus, and Resistance Genes in Soils Irrigated with Wastewater for Zero to 100 Years in Central Mexico

PubMed Central

Siebe, Christina; Willaschek, Elisha; Sakinc, Tuerkan; Huebner, Johannes; Amelung, Wulf; Grohmann, Elisabeth; Siemens, Jan

2012-01-01

Irrigation with wastewater releases pharmaceuticals, pathogenic bacteria, and resistance genes, but little is known about the accumulation of these contaminants in the environment when wastewater is applied for decades. We sampled a chronosequence of soils that were variously irrigated with wastewater from zero up to 100 years in the Mezquital Valley, Mexico, and investigated the accumulation of ciprofloxacin, enrofloxacin, sulfamethoxazole, trimethoprim, clarithromycin, carbamazepine, bezafibrate, naproxen, diclofenac, as well as the occurrence of Enterococcus spp., and sul and qnr resistance genes. Total concentrations of ciprofloxacin, sulfamethoxazole, and carbamazepine increased with irrigation duration reaching 95% of their upper limit of 1.4 µg/kg (ciprofloxacin), 4.3 µg/kg (sulfamethoxazole), and 5.4 µg/kg (carbamazepine) in soils irrigated for 19–28 years. Accumulation was soil-type-specific, with largest accumulation rates in Leptosols and no time-trend in Vertisols. Acidic pharmaceuticals (diclofenac, naproxen, bezafibrate) were not retained and thus did not accumulate in soils. We did not detect qnrA genes, but qnrS and qnrB genes were found in two of the irrigated soils. Relative concentrations of sul1 genes in irrigated soils were two orders of magnitude larger (3.15×10−3±0.22×10−3 copies/16S rDNA) than in non-irrigated soils (4.35×10−5±1.00×10−5 copies/16S rDNA), while those of sul2 exceeded the ones in non-irrigated soils still by a factor of 22 (6.61×10–4±0.59×10−4 versus 2.99×10−5±0.26×10−5 copies/16S rDNA). Absolute numbers of sul genes continued to increase with prolonging irrigation together with Enterococcus spp. 23S rDNA and total 16S rDNA contents. Increasing total concentrations of antibiotics in soil are not accompanied by increasing relative abundances of resistance genes. Nevertheless, wastewater irrigation enlarges the absolute concentration of resistance genes in soils due to a long-term increase in total microbial biomass. PMID:23049795

Accumulation of pharmaceuticals, Enterococcus, and resistance genes in soils irrigated with wastewater for zero to 100 years in central Mexico.

PubMed

Dalkmann, Philipp; Broszat, Melanie; Siebe, Christina; Willaschek, Elisha; Sakinc, Tuerkan; Huebner, Johannes; Amelung, Wulf; Grohmann, Elisabeth; Siemens, Jan

2012-01-01

Irrigation with wastewater releases pharmaceuticals, pathogenic bacteria, and resistance genes, but little is known about the accumulation of these contaminants in the environment when wastewater is applied for decades. We sampled a chronosequence of soils that were variously irrigated with wastewater from zero up to 100 years in the Mezquital Valley, Mexico, and investigated the accumulation of ciprofloxacin, enrofloxacin, sulfamethoxazole, trimethoprim, clarithromycin, carbamazepine, bezafibrate, naproxen, diclofenac, as well as the occurrence of Enterococcus spp., and sul and qnr resistance genes. Total concentrations of ciprofloxacin, sulfamethoxazole, and carbamazepine increased with irrigation duration reaching 95% of their upper limit of 1.4 µg/kg (ciprofloxacin), 4.3 µg/kg (sulfamethoxazole), and 5.4 µg/kg (carbamazepine) in soils irrigated for 19-28 years. Accumulation was soil-type-specific, with largest accumulation rates in Leptosols and no time-trend in Vertisols. Acidic pharmaceuticals (diclofenac, naproxen, bezafibrate) were not retained and thus did not accumulate in soils. We did not detect qnrA genes, but qnrS and qnrB genes were found in two of the irrigated soils. Relative concentrations of sul1 genes in irrigated soils were two orders of magnitude larger (3.15 × 10(-3) ± 0.22 × 10(-3) copies/16S rDNA) than in non-irrigated soils (4.35 × 10(-5)± 1.00 × 10(-5) copies/16S rDNA), while those of sul2 exceeded the ones in non-irrigated soils still by a factor of 22 (6.61 × 10(-4) ± 0.59 × 10(-4) versus 2.99 × 10(-5) ± 0.26 × 10(-5) copies/16S rDNA). Absolute numbers of sul genes continued to increase with prolonging irrigation together with Enterococcus spp. 23S rDNA and total 16S rDNA contents. Increasing total concentrations of antibiotics in soil are not accompanied by increasing relative abundances of resistance genes. Nevertheless, wastewater irrigation enlarges the absolute concentration of resistance genes in soils due to a long-term increase in total microbial biomass.

Occurrence and transformation of veterinary antibiotics and antibiotic resistance genes in dairy manure treated by advanced anaerobic digestion and conventional treatment methods.

PubMed

Wallace, Joshua S; Garner, Emily; Pruden, Amy; Aga, Diana S

2018-05-01

Manure treatment technologies are rapidly developing to minimize eutrophication of surrounding environments and potentially decrease the introduction of antibiotics and antibiotic resistant genes (ARGs) into the environment. While laboratory and pilot-scale manure treatment systems boast promising results, antibiotic and ARG removals in full-scale systems receiving continuous manure input have not been evaluated. The effect of treatment on ARGs is similarly lacking. This study examines the occurrence and transformation of sulfonamides, tetracyclines, tetracycline degradation products, and related ARGs throughout a full-scale advanced anaerobic digester (AAD) receiving continuous manure and antibiotic input. Manure samples were collected throughout the AAD system to evaluate baseline antibiotic and ARG input (raw manure), the effect of hygenization (post-pasteurized manure) and anaerobic digestion (post-digestion manure) on antibiotic and ARG levels. Antibiotics were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the ARGs tet(O), tet(W), sul1 and sul2 were analyzed by quantitative polymerase chain reaction (Q-PCR). Significant reductions in the concentrations of chlortetracycline, oxytetracycline, tetracycline and their degradation products were observed in manure liquids following treatment (p < 0.001), concomitant to significant increases in manure solids (p < 0.001). These results suggest sorption is the major removal route for tetracyclines during AAD. Significant decreases in the epimer-to-total residue ratios for chlortetracycline and tetracycline in manure solids further indicate degradation is desorption-limited. Moreover, sul1 and sul2 copies decreased significantly (p < 0.001) following AAD in the absence of sulfonamide antibiotics, while tetracyclines-resistant genes remained unchanged. A cross-sectional study of dairy farms utilizing natural aeration and liquid-solid separation treatments was additionally performed to compare levels of antibiotics and ARGs found in AAD with the levels in common manure management systems. The concentration of antibiotics in raw manure varied greatly between farms while minimal differences in ARGs were observed. However, significant (p < 0.01) differences in the levels of antibiotics and ARGs (except tet(W)) were observed in the effluents from the three different manure management systems. Copyright © 2018 Elsevier Ltd. All rights reserved.

Brazil: Duck Lagoon

Atmospheric Science Data Center

2013-04-18

article title:  Brazil - The Duck Lagoon     View Larger Image ... the Brazilian state of Rio Grande do Sul, translates to "the Duck Lagoon". It was named by 16th century Jesuit settlers, who asked the King ...

Sourcing Brazilian marijuana by applying IRMS analysis to seized samples.

PubMed

Shibuya, Elisa K; Souza Sarkis, Jorge E; Neto, Osvaldo Negrini; Moreira, Marcelo Z; Victoria, Reynaldo L

2006-06-27

The stable carbon and nitrogen isotopic ratios were measured in marijuana samples (Cannabis sativa L.) seized by the law enforcement officers in the three Brazilian production sites: Pernambuco and Bahia (the country's Northeast known as Marijuana Polygon), Pará (North or Amazon region) and Mato Grosso do Sul (Midwest). These regions are regarded as different with respect to climate and water availability, factors which impact upon the isotope fractionations of these elements within plants. It was possible to differentiate samples from the dry regions (Marijuana Polygon) from those from Mato Grosso do Sul and Pará, that present heavier rainfall. The results were in agreement with the climatic conditions of the suspected regions of origin and this demonstrates that seized samples can be used to identify the isotopic signatures of marijuana from the main producing regions in Brazil.

[Does time spent traveling to regional hub cities to receive healthcare influence mortality in small towns in Rio Grande do Sul State, Brazil?

PubMed

Zuanazzi, Pedro Tonon; Cabral, Pedro Henrique Vargas; Stella, Milton André; Moraes, Gustavo Inácio de

2017-12-18

The current study aims to determine whether the time spent travelling to regional hub cities to receive healthcare affects mortality from avoidable causes and the standardized crude mortality rate in towns with up to 5,000 inhabitants in Rio Grande do Sul State, Brazil. Without adjusting for control variables, the longest time spent to reach cities with 100,000 inhabitants or more was associated with an increase in both rates. However, while the pattern in the avoidable mortality rate was similar after including controls, the standardized crude mortality rate reversed its signal. This suggests that if other socioeconomic and healthcare characteristics are kept constant, the distance to reference cities is associated with both a reduction in deaths from avoidable causes and an increase in other causes of death.

Independent Life Skills among psychosocial care network users of Rio Grande do Sul, Brazil.

PubMed

Rodrigues, Cândida Garcia Sinott Silveira; Jardim, Vanda Maria da Rosa; Kantorski, Luciane Prado; Coimbra, Valeria Cristina Christello; Treichel, Carlos Alberto Dos Santos; Francchini, Beatriz; Bretanha, Andreia Ferreira; Neutzling, Aline Dos Santos

2016-08-01

This is a cross-sectional study that aims to identify the prevalence of lower independent living skills and their associations in 390 users of psychiatric community-based services in the state Rio Grande do Sul, Brazil. For tracing the outcome it was used the "scale Independent Living Skills Survey", adopting a cut-off value lower than 2. The crude and adjusted analyses were conducted on binary logistic regressions and they considered a hierarchical model developed through a systematic literature review. In adjusted analysis the level of the same variables were adjusted to each other and to previous levels. The statistical significance remained as a < 0.05 p-value. The prevalence of smaller independent living skills was 33% and their associations were: younger age; no partner; lower education; resident at SRT; diagnosis of schizophrenia and younger diagnosis.

Army Command and Control Study - 82 (ACCS-82). Volume I. Executive Summary and Study Report.

DTIC Science & Technology

1979-09-30

36H 7.9 .409 7.9 .409 Sul -rOTAL iuo.4 .904 .946 .9Y4 1.u53 1.079 4 10.6 42.1 045 4.1 .183 4.9 219 4 .4 .196 5.5 .245 6.5 .290 25 .154 2.6 .160 4.1 .252...100.0 b 39.4 (1) 44.1 .044 3.9 .173 3.9 .173 6.4 .214 7.1 .315 7.4 .328 (2) b5.9 .056 0.5 .19? 3.2 .180 b. .2yu 6.5 .36b 6.8 .382 SUl -7OTAL 100.0 100.0...PLANNERS + 162.6 - + 18.0 + 180.6 CORPS RIO -AFFILIA- TION + 89.2 - + 10.4 + 99.6 DIV R/O-AFFILIATION + 446.1 - + 52.1 + 498.2 INSTALLATION MOB + 624.5

Co-occurrence of mobile genetic elements and antibiotic resistance genes in municipal solid waste landfill leachates: A preliminary insight into the role of landfill age.

PubMed

Yu, Zhuofeng; He, Pinjing; Shao, Liming; Zhang, Hua; Lü, Fan

2016-12-01

Since municipal solid waste (MSW) landfill harbours miscellaneous wastes, pollutants and microorganisms, it gradually becomes a huge potential reservoir for breeding antibiotic resistance genes (ARGs). The objective of this study was to determine the prevalence and diversity of ARGs associated with various mobile genetic elements (MGEs) in MSW landfill leachates. The relationship of ARGs with leachate characteristics was also studied to explore the influence of landfill age. Seven sulfonamides (sulfapyridine, sulfadiazine, sulfathiazole, sulfamethoxazole, sulfamerazine, sulfamethazine and sulfaquinoxaline), three encoded ARGs (sul-I, sul-II and sul-III) and four types of MGEs (plasmids, transposons, integrons and insertion sequences) were quantified in leachates with landfill ages ranging from 3 months-6 years. ARGs increased to an absolute concentration of 10 6 copies/μL and were positively correlated (p < 0.05) to MGEs. Significant correlations (p < 0.05) were also discovered among ARGs and the increasing humic acids, heavy metals (Zn, Cu and Co) and antibiotics (except for sulfathiazole and sulfaquinoxaline), implying landfilling might contribute to the enrichment of ARGs in the long-term. Non-target full scans revealed the role of persistent unknown compounds in stimulating the ARGs dissemination. Overall, this study demonstrates the exacerbation of ARGs pollution in landfill environment and a detailed delineation of the complex inter-relationships between ARGs and the substances harbouring in landfills is badly required. Copyright © 2016 Elsevier Ltd. All rights reserved.

Engineering the growth pattern and cell morphology for enhanced PHB production by Escherichia coli.

PubMed

Wu, Hong; Chen, Jinchun; Chen, Guo-Qiang

2016-12-01

E. coli JM109∆envC∆nlpD deleted with genes envC and nlpD responsible for degrading peptidoglycan (PG) led to long filamentous cell shapes. When cell fission ring location genes minC and minD of Escherichia coli were deleted, E. coli JM109∆minCD changed the cell growth pattern from binary division to multiple fissions. Bacterial morphology can be further engineered by overexpressing sulA gene resulting in inhibition on FtsZ, thus generating very long cellular filaments. By overexpressing sulA in E. coli JM109∆envC∆nlpD and E. coli JM109∆minCD harboring poly(3-hydroxybutyrate) (PHB) synthesis operon phbCAB encoded in plasmid pBHR68, respectively, both engineered cells became long filaments and accumulated more PHB compared with the wild-type. Under same shake flask growth conditions, E. coli JM109∆minCD (pBHR68) overexpressing sulA grown in multiple fission pattern accumulated approximately 70 % PHB in 9 g/L cell dry mass (CDM), which was significantly higher than E. coli JM109∆envC∆nlpD and the wild type, that produced 7.6 g/L and 8 g/L CDM containing 64 % and 51 % PHB, respectively. Results demonstrated that a combination of the new division pattern with elongated shape of E. coli improved PHB production. This provided a new vision on the enhanced production of inclusion bodies.

Paracoccidioidomycosis in southern Rio Grande do Sul: a retrospective study of histopathologically diagnosed cases.

PubMed

de Souza, Silvana Pereira; Jorge, Valéria Magalhães; Xavier, Melissa Orzechowski

2014-01-01

Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the fungus Paracoccidioides brasiliensis and is endemic to Brazil. The aim of this study was to perform a retrospective analysis of the PCM cases in the countryside south of Rio Grande do Sul, Brazil. The files from four histopathology laboratories located in the city of Pelotas were obtained, and all of the epidemiological and clinical data from the PCM diagnosed cases were collected for analysis. A total of 123 PCM cases diagnosed between 1966 and 2009 were selected. Of these patients, 104 (84.5%) were male, and 17 were female. The patients ranged from 02 to 92 years of age. Fifty-two cases (41.9%) were obtained from the oral pathology laboratory, and the remaining 71 cases (58.1%) were obtained from the three general pathology laboratories. Of all of the patients studied, 65.2% lived in rural zones and worked in agriculture or other related fields. Data on the evolution of this disease was available for 43 cases, and the time frame ranged from 20 to 2920 days (mean = 572.3 days). An accurate diagnosis performed in less than 30 days only occurred in 21% of the cases. PCM is endemic to the countryside of Rio Grande do Sul. Therefore, it is recommended that PCM be included as a differential diagnosis, mainly for individuals between 30 and 60 years of age, living in rural zones and who have respiratory signs and associated-oropharyngeal lesions.

Variations in the Prevalence of Risk Factors for Coronary Artery Disease in Rio Grande do Sul-Brazil: A Comparative Analysis between 2002 and 2014.

PubMed

Gus, Iseu; Ribeiro, Rodrigo Antonini; Kato, Sérgio; Bastos, Juliano; Medina, Claudio; Zazlavsky, Claudio; Portal, Vera Lucia; Timmers, Rita; Markoski, Melissa Medeiros; Gottschall, Carlos Antônio Mascia

2015-12-01

Due to the importance of coronary artery disease (CAD), continuous investigation of the risk factors (RFs) is needed. To evaluate the prevalence of RFs for CAD in cities in Rio Grande do Sul State, and compare it with that reported in a similar study conducted in the same cities in 2002. Cross-sectional study on 1,056 healthy adults, investigating the prevalence and absolute and relative frequencies of the following RFs for CAD: obesity, systemic arterial hypertension (SAH), dyslipidemias, smoking, sedentary lifestyle, diabetes mellitus, and family history, as well as age and sex. Data was collected in 19 cities, host of the Offices of the Regional Coordinators of Health, as in the 2002 study. Twenty-six percent of the sample consisted of older adults and 57% were women. The prevalence of sedentary lifestyle was 44%, history family 50%, smoking 23%, overweight/obesity 68%, dyslipidemia (high cholesterol levels) 43%, SAH 40%, and diabetes 11%. When compared to the 2002 study, the prevalence of active smoking and sedentary behavior decreased, whereas the prevalence of hypertension, dyslipidemia and obesity increased. Obesity is the most prevalent RF in women, and SAH the most prevalent in men. The prevalence of RFs for CAD in Rio Grande do Sul State remains high. Hypertension, obesity and dyslipidemia are still prevalent and require major prevention programs. Smoking and physical inactivity have decreased in the state, suggesting the efficacy of related campaigns.

Analysis of extreme wave events on the southern coast of Brazil

NASA Astrophysics Data System (ADS)

Guimarães, P. V.; Farina, L.; Toldo, E. E., Jr.

2014-12-01

Using the wave model SWAN (simulating waves nearshore), high waves on the southwestern Atlantic generated by extra-tropical cyclones are simulated from 2000 to 2010, and their impact on the Rio Grande do Sul (RS) coast is studied. The modeled waves are compared with buoy data and good agreement is found. The six extreme events in the period that presented significant wave heights above 5 m, on a particular point of interest, are investigated in detail. It is found that the cyclogenetic pattern between the latitudes 31.5 and 34° S is the most favorable for developing high waves. Hovmöller diagrams for deep water show that the region between the south of Rio Grande do Sul up to a latitude of 31.5° S is the most energetic during a cyclone's passage, although the event of May 2008 indicates that the location of this region can vary, depending on the cyclone's displacement. On the other hand, the Hovmöller diagrams for shallow water show that the different shoreface morphologies were responsible for focusing or dissipating the waves' energy; the regions found are in agreement with the observations of erosion and progradation regions. It can be concluded that some of the urban areas of the beaches of Hermenegildo, Cidreira, Pinhal, Tramandaí, Imbé and Torres have been more exposed during the extreme wave events on the Rio Grande do Sul coast, and are more vulnerable to this natural hazard.

Paracoccidioidomycosis in southern Rio Grande do Sul: A retrospective study of histopathologically diagnosed cases

PubMed Central

de Souza, Silvana Pereira; Jorge, Valéria Magalhães; Xavier, Melissa Orzechowski

2014-01-01

Paracoccidioidomycosis (PCM) is a systemic mycosis caused by the fungus Paracoccidioides brasiliensis and is endemic to Brazil. The aim of this study was to perform a retrospective analysis of the PCM cases in the countryside south of Rio Grande do Sul, Brazil. The files from four histopathology laboratories located in the city of Pelotas were obtained, and all of the epidemiological and clinical data from the PCM diagnosed cases were collected for analysis. A total of 123 PCM cases diagnosed between 1966 and 2009 were selected. Of these patients, 104 (84.5%) were male, and 17 were female. The patients ranged from 02 to 92 years of age. Fifty-two cases (41.9%) were obtained from the oral pathology laboratory, and the remaining 71 cases (58.1%) were obtained from the three general pathology laboratories. Of all of the patients studied, 65.2% lived in rural zones and worked in agriculture or other related fields. Data on the evolution of this disease was available for 43 cases, and the time frame ranged from 20 to 2920 days (mean = 572.3 days). An accurate diagnosis performed in less than 30 days only occurred in 21% of the cases. PCM is endemic to the countryside of Rio Grande do Sul. Therefore, it is recommended that PCM be included as a differential diagnosis, mainly for individuals between 30 and 60 years of age, living in rural zones and who have respiratory signs and associated-oropharyngeal lesions. PMID:24948940

Thymineless death is inhibited by CsrA in Escherichia coli lacking the SOS response.

PubMed

Hamilton, Holly M; Wilson, Ray; Blythe, Martin; Nehring, Ralf B; Fonville, Natalie C; Louis, Edward J; Rosenberg, Susan M

2013-11-01

Thymineless death (TLD) is the rapid loss of colony-forming ability in bacterial, yeast and human cells starved for thymine, and is the mechanism of action of common chemotherapeutic drugs. In Escherichia coli, significant loss of viability during TLD requires the SOS replication-stress/DNA-damage response, specifically its role in inducing the inhibitor of cell division, SulA. An independent RecQ- and RecJ-dependent TLD pathway accounts for a similarly large additional component of TLD, and a third SOS- and RecQ/J-independent TLD pathway has also been observed. Although two groups have implicated the SOS-response in TLD, an SOS-deficient mutant strain from an earlier study was found to be sensitive to thymine deprivation. We performed whole-genome resequencing on that SOS-deficient strain and find that, compared with the SOS-proficient control strain, it contains five mutations in addition to the SOS-blocking lexA(Ind(-)) mutation. One of the additional mutations, csrA, confers TLD sensitivity specifically in SOS-defective strains. We find that CsrA, a carbon storage regulator, reduces TLD in SOS- or SulA-defective cells, and that the increased TLD that occurs in csrA(-) SOS-defective cells is dependent on RecQ. We consider a hypothesis in which the modulation of nucleotide pools by CsrA might inhibit TLD specifically in SOS-deficient (SulA-deficient) cells. Copyright © 2013 Elsevier B.V. All rights reserved.

Potential pathogens, antimicrobial patterns and genotypic diversity of Escherichia coli isolates in constructed wetlands treating swine wastewater.

PubMed

Ibekwe, A M; Murinda, Shelton E; DebRoy, Chitrita; Reddy, Gudigopura B

2016-02-01

Escherichia coli populations originating from swine houses through constructed wetlands were analyzed for potential pathogens, antimicrobial susceptibility patterns, and genotypic diversity. Escherichia coli isolates (n = 493) were screened for the presence of the following virulence genes: stx1, stx2 and eae (Shiga toxin-producing E. coli [STEC]), heat-labile enterotoxin (LT) genes and heat stable toxin STa and STb (enterotoxigenic E. coli (ETEC), cytotoxin necrotizing factors 1 and 2 (cnf1 and cnf2 [necrotoxigenic E. coli- NTEC]), as well as O and H antigens, and the presence of the antibiotic resistance genes blaTEM, blaSHV, blaCMY-2, tet A, tet B, tet C, mph(A), aadA, StrA/B, sul1, sul2 and sul3. The commensal strains were further screened for 16 antimicrobials and characterized by BOX AIR-1 PCR for unique genotypes. The highest antibiotic resistance prevalence was for tetracycline, followed by erythromycin, ampicillin, streptomycin, sulfisoxazole and kanamycin. Our data showed that most of the isolates had high distribution of single or multidrug-resistant (MDR) genotypes. Therefore, the occurrence of MDR E. coli in the wetland is a matter of great concern due to possible transfer of resistance genes from nonpathogenic to pathogenic strains or vice versa in the environment. Published by Oxford University Press on behalf of FEMS 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.

A new species of Pareiorhaphis (Siluriformes: Loricariidae) from the headwaters of the Arroio Garapiá, coastal drainage of Rio Grande do Sul state, Brazil.

PubMed

Pereira, Edson H L; Lehmann, Pablo A; Schvambach, Lucas J; Reis, Roberto E

2015-10-30

Pareiorhaphis garapia, new species, is described based on specimens collected in the headwaters of the Arroio Garapiá, Rio Maquiné basin, a coastal drainage of Rio Grande do Sul State, southern Brazil. The new species is distinguished from all other Pareiorhaphis species in having the nuchal plate covered by thick skin, the exposed posterior process of the cleithrum comparatively narrow, and the last segment of the preopercular ramus of the latero-sensory canal reduced to an ossified tubule. The absence of a dorsal-fin spinelet, the reduced number of plates in the dorsal and mid-dorsal series of lateral plates, and morphometric traits also distinguish the new species from its congeners. The restricted geographic distribution of P. garapia, endemic to a headwater stream of the Rio Maquiné basin, and the syntopic occurrence of P. nudulus are discussed.

Tuberculosis among prison staff in Rio Grande do Sul.

PubMed

Busatto, Caroline; Nunes, Luciana de Souza; Valim, Andréia Rosane de Moura; Valença, Mariana Soares; Krug, Suzane Frantz; Becker, Daniela; Allgayer, Manuela Filter; Possuelo, Lia Gonçalves

2017-04-01

to evaluate the risk of infection and illness caused by Mycobacterium tuberculosis among health care and security staff in prisons in two regions of Rio Grande do Sul (RS). cross-sectional study involving prison staff. An interview and sputum smear microscopy and culture were performed. Latent infection was evaluated according to the result of the tuberculin test (TT), self-referred. among staff who had a TT, 10 (83.3%) in the central region and 2 (16.7%) in the southern region were considered reactors. Length of employment among prison officers who reacted to TT was 15.3 years, and among health care workers, 4.1 years (p = 0.01). No cases of active tuberculosis (TB) were identified. prevalence of latent TB was 27.9%. Length of employment between different professional categories and their working regions was considered a risk factor for latent TB.

[Use of dental services by preschool children in Canela, Rio Grande do Sul State, Brazil].

PubMed

Kramer, Paulo Floriani; Ardenghi, Thiago Machado; Ferreira, Simone; Fischer, Laura de Almeida; Cardoso, Luciana; Feldens, Carlos Alberto

2008-01-01

The aim of this study was to assess the use of dental services and age at first dental visit in preschool children in Canela, Rio Grande do Sul State, Brazil. A representative sample of under-five children was surveyed on National Children's Vaccination Day. Children's parents completed questionnaires containing socio-demographic data and age at first dental visit. Data were analyzed using multiple logistic regression. 192 children were examined. 13.3% of the sample had already visited the dentist at least once, but only 4.3% had their first dental visit by one year of age. The number of children who had already visited a dentist increased with age. Girls showed higher odds of having visited a dentist (OR = 1.46; 95%CI: 1.01-2.1). Public health strategies are needed to determine the effectiveness of health promotion and improve the use of dental services by preschool children.

Participatory methodologies in research with children: creative and innovative approaches.

PubMed

Pereira, Viviane Ribeiro; Coimbra, Valéria Cristina Christello; Cardoso, Clarissa de Souza; Oliveira, Naiana Alves; Vieira, Ana Cláudia Garcia; Nobre, Márcia de Oliveira; Nino, Magda Eliete Lamas

2017-05-18

To describe the use of participatory methodologies in research with children. Experience report with a qualitative approach, conducted with children between six and eleven years of age, from a municipal school in Pelotas and in the Psychosocial Children and Youth Care Center, in São Lourenço do Sul, both municipalities of the Rio Grande do Sul State. Data collection was based on records made in field and observation diaries, held from April to July 2016. The report pointed out that the Photovoice promoted motivation in the group, in addition to increasing the self-esteem and self-confidence of children. The Five Field Map made it possible to help children express feelings through the game. Photovoice and the Five Field Map are seen as tools that enable new methodological approaches in research with children, facilitating the construction of the proposed activities aimed at innovative and creative research processes in health/nursing.

Variations in the Prevalence of Risk Factors for Coronary Artery Disease in Rio Grande do Sul-Brazil: A Comparative Analysis between 2002 and 2014

PubMed Central

Gus, Iseu; Ribeiro, Rodrigo Antonini; Kato, Sérgio; Bastos, Juliano; Medina, Claudio; Zazlavsky, Claudio; Portal, Vera Lucia; Timmers, Rita; Markoski, Melissa Medeiros; Gottschall, Carlos Antônio Mascia

2015-01-01

Background Due to the importance of coronary artery disease (CAD), continuous investigation of the risk factors (RFs) is needed. Objective To evaluate the prevalence of RFs for CAD in cities in Rio Grande do Sul State, and compare it with that reported in a similar study conducted in the same cities in 2002. Methods Cross-sectional study on 1,056 healthy adults, investigating the prevalence and absolute and relative frequencies of the following RFs for CAD: obesity, systemic arterial hypertension (SAH), dyslipidemias, smoking, sedentary lifestyle, diabetes mellitus, and family history, as well as age and sex. Data was collected in 19 cities, host of the Offices of the Regional Coordinators of Health, as in the 2002 study. Results Twenty-six percent of the sample consisted of older adults and 57% were women. The prevalence of sedentary lifestyle was 44%, history family 50%, smoking 23%, overweight/obesity 68%, dyslipidemia (high cholesterol levels) 43%, SAH 40%, and diabetes 11%. When compared to the 2002 study, the prevalence of active smoking and sedentary behavior decreased, whereas the prevalence of hypertension, dyslipidemia and obesity increased. Obesity is the most prevalent RF in women, and SAH the most prevalent in men. Conclusions The prevalence of RFs for CAD in Rio Grande do Sul State remains high. Hypertension, obesity and dyslipidemia are still prevalent and require major prevention programs. Smoking and physical inactivity have decreased in the state, suggesting the efficacy of related campaigns. PMID:26761368

Sulforaphane enhances the activity of the Nrf2-ARE pathway and attenuates inflammation in OxyHb-induced rat vascular smooth muscle cells.

PubMed

Zhao, X-D; Zhou, Y-T; Lu, X-J

2013-09-01

A growing body of evidence indicates that the nuclear factor erythroid 2-related factor 2-antioxidant response element (Nrf2-ARE) pathway plays a protective role in many physiological stress processes such as inflammatory damage, oxidative stress, and the accumulation of toxic metabolites, which are all involved in the cerebral vasospasm following subarachnoid hemorrhage (SAH). We hypothesized that the Nrf2-ARE pathway might have a protective role in cerebral vasospasm following SAH. In our study, we investigate whether the oxyhemoglobin (OxyHb) can induce the activation of the Nrf2-ARE pathway in vascular smooth muscle cells (VSMCs), and evaluate the modulatory effects of sulforaphane (SUL) on OxyHb-induced inflammation in VSMCs. As a result, both the protein level and the mRNA level of the nuclear Nrf2 were significantly increased, while the mRNA levels of two Nrf2-regulated gene products, both heme oxygenase-1 and NAD(P)H: quinone oxidoreductase-1, were also up-regulated in VSMCs induced with OxyHb. A marked increase of inflammatory cytokines such as IL-1β, IL-6 and TNF-α release was observed at 48 h after cells were treated with OxyHb. SUL enhanced the activity of the Nrf2-ARE pathway and suppressed cytokine release. Our results indicate that the Nrf2-ARE pathway was activated in OxyHb-induced VSMCs. SUL suppressed cytokine release via the activation of the Nrf2-ARE pathway in OxyHb-induced VSMCs.

Quantification and molecular characterization of Salmonella isolated from food samples involved in salmonellosis outbreaks in Rio Grande do Sul, Brazil

PubMed Central

Mürmann, Lisandra; dos Santos, Maria Cecília; Longaray, Solange Mendes; Both, Jane Mari Corrêa; Cardoso, Marisa

2008-01-01

Data concerning the prevalence and populations of Salmonella in foods implicated in outbreaks may be important to the development of quantitative microbial risk assessments of individual food products. In this sense, the objective of the present study was to assess the amount of Salmonella sp. in different foods implicated in foodborne outbreaks in Rio Grande do Sul occurred in 2005 and to characterize the isolated strains using phenotypic and genotypic methods. Nineteen food samples involved in ten foodborne outbreaks occurred in 2005, and positive on Salmonella isolation at the Central Laboratory of the Health Department of the State of Rio Grande do Sul, were included in this study. Food samples were submitted to estimation of Salmonella using the Most Probable Number (MPN) technique. Moreover, one confirmed Salmonella colony of each food sample was serotyped, characterized by its XbaI-macrorestriction profile, and submitted to antimicrobial resistance testing. Foods containing eggs, mayonnaise or chicken were contaminated with Salmonella in eight outbreaks. Higher counts (>107 MPN.g-1) of Salmonella were detected mostly in foods containing mayonnaise. The isolation of Salmonella from multiple food items in five outbreaks probably resulted from the cross-contamination, and the high Salmonella counts detected in almost all analyzed samples probably resulted from storing in inadequate temperature. All strains were identified as S. Enteritidis, and presented a unique macrorestriction profile, demonstrating the predominance of one clonal group in foods involved in the salmonellosis outbreaks. A low frequency of antimicrobial resistant S. Enteritidis strains was observed and nalidixic acid was the only resistance marker detected. PMID:24031261

Analysis of antibiotic multi-resistant bacteria and resistance genes in the effluent of an intensive shrimp farm (Long An, Vietnam).

PubMed

Pham, Thi Thu Hang; Rossi, Pierre; Dinh, Hoang Dang Khoa; Pham, Ngoc Tu Anh; Tran, Phuong Anh; Ho, To Thi Khai Mui; Dinh, Quoc Tuc; De Alencastro, Luiz Felippe

2018-05-15

In Vietnam, intensive shrimp farms heavily rely on a wide variety of antibiotics (ABs) to treat animals or prevent disease outbreak. Potential for the emergence of multi-resistant bacteria is high, with the concomitant contamination of adjacent natural aquatic habitats used for irrigation and drinking water, impairing in turn human health system. In the present study, quantification of AB multi-resistant bacteria was carried out in water and sediment samples from effluent channels connecting a shrimp farming area to the Vam Co River (Long An Province, Vietnam). Bacterial strains, e.g. Klebsiella pneumoniae and Aeromonas hydrophila, showing multi-resistance traits were isolated. Molecular biology analysis showed that these strains possessed from four to seven different AB resistance genes (ARGs) (e.g. sul1, sul2, qnrA, ermB, tetA, aac(6)lb, dfrA1, dfr12, dfrA5), conferring multidrug resistance capacity. Sequencing of plasmids present within these multi-resistant strains led to the identification of a total of forty-one resistance genes, targeting nine AB groups. qPCR analysis on the sul2 gene revealed the presence of high copy numbers in the effluent channel connecting to the Vam Co River. The results of the present study clearly indicated that multi-resistant bacteria present in intensive shrimp cultures may disseminate in the natural environment. This study offered a first insight in the impact of plasmid-born ARGs and the related pathogenic bacteria that could emerged due to inappropriate antibiotic utilization in South Vietnam. Copyright © 2018 Elsevier Ltd. All rights reserved.

Proliferative effect of plants used for wound healing in Rio Grande do Sul state, Brazil.

PubMed

Alerico, Gabriela C; Beckenkamp, Aline; Vignoli-Silva, Márcia; Buffon, Andréia; von Poser, Gilsane L

2015-12-24

Wounds are normally resolved in a few days, but chronic wounds represent a major burden because of economic and social factors. Thereby, the search for new agents is ongoing and natural products become a great target. Also, Brazil as a consumer of herbal medicines with rich social diversity is promising for ethnopharmacological studies. The study aims to find the plants popularly used for wound healing purposes in Rio Grande do Sul state, and test the traditional knowledge through an in vitro screening. Ethnobotanical studies from state of Rio Grande do Sul were analyzed to find the most used plants to treat wounds. The selected species were collected, identified and ethanolic and aqueous extracts were prepared. After, proliferative capacity was accessed by MTT assay in a keratinocyte cell line (HaCaT). The survey comprehended almost all state regions and led to 117 plant species from 85 genera, from which 14 were selected for in vitro testing. Aqueous extracts from Achyrocline satureioides DC Lam., Matricaria recutita L., Melia azedarach L. and Mirabilis jalapa L. demonstrated the ability to stimulate keratinocyte growth up to 120% in concentrations of 25 µg/mL and 50 µg/mL. The ethanolic extract of A. satureioides was able to stimulate keratinocyte and fibroblast proliferation on the lower concentration tested, 1 µg/mL, being the most promising species. The traditional knowledge collected from the ethnobotanical studies was accessed by in vitro investigation and extracts from Achyrocline satureioides, Matricaria recutita, Melia azedarach and Mirabilis jalapa can influence positively cell proliferation. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Antibiotic resistance genes in surface water of eutrophic urban lakes are related to heavy metals, antibiotics, lake morphology and anthropic impact.

PubMed

Yang, Yuyi; Xu, Chen; Cao, Xinhua; Lin, Hui; Wang, Jun

2017-08-01

Urban lakes are impacted by heavy human activities and represent potential reservoirs for antibiotic resistance genes. In this study, six urban lakes in Wuhan, central China were selected to analyze the distribution of sulfonamide resistance (sul) genes, tetracycline resistance (tet) genes and quinolone resistance (qnr) genes and their relationship with heavy metals, antibiotics, lake morphology and anthropic impact. sul1 and sul2 were detected in all six lakes and dominated the types of antibiotic resistance genes, which accounted for 86.28-97.79% of the total antibiotic resistance gene abundance. For eight tested tet genes, antibiotic efflux pumps (tetA, tetB, tetC, and tetG) genes were all observed in six lakes and had higher relative abundance than ribosomal protection protein genes (tetM and tetQ). For 4 plasmid mediated quinolone resistance genes, only qnrD is found in all six lakes. The class I integron (intI1) is also found to be a very important media for antibiotic resistance gene propagation in urban lakes. The results of redundancy analysis and variation partitioning analysis showed that antibiotic and co-selection with heavy metals were the major factors driving the propagation of antibiotic resistance genes in six urban lakes. The heavily eutrophic Nanhu Lake and Shahu Lake which located in a high density building area with heavy human activities had the higher relative abundance of total antibiotic resistance genes. Our study could provide a useful reference for antibiotic resistance gene abundance in urban lakes with high anthropic impact.

Antibiotic resistance genes fate and removal by a technological treatment solution for water reuse in agriculture.

PubMed

Luprano, Maria Laura; De Sanctis, Marco; Del Moro, Guido; Di Iaconi, Claudio; Lopez, Antonio; Levantesi, Caterina

2016-11-15

In order to mitigate the potential effects on the human health which are associated to the use of treated wastewater in agriculture, antibiotic resistance genes (ARGs) are required to be carefully monitored in wastewater reuse processes and their spread should be prevented by the development of efficient treatment technologies. Objective of this study was the assessment of ARGs reduction efficiencies of a novel technological treatment solution for agricultural reuse of municipal wastewaters. The proposed solution comprises an advanced biological treatment (Sequencing Batch Biofilter Granular Reactor, SBBGR), analysed both al laboratory and pilot scale, followed by sand filtration and two different disinfection final stages: ultraviolet light (UV) radiation and peracetic acid (PAA) treatments. By Polymerase Chain Reaction (PCR), the presence of 9 ARGs (ampC, mecA, ermB, sul1, sul2, tetA, tetO, tetW, vanA) were analysed and by quantitative PCR (qPCR) their removal was determined. The obtained results were compared to the reduction of total bacteria (16S rDNA gene) and of a faecal contamination indicator (Escherichia coli uidA gene). Only four of the analysed genes (ermB, sul1, sul2, tetA) were detected in raw wastewater and their abundance was estimated to be 3.4±0.7 x10(4) - 9.6±0.5 x10(9) and 1.0±0.3 x10(3) to 3.0±0.1 x10(7) gene copies/mL in raw and treated wastewaters, respectively. The results show that SBBGR technology is promising for the reduction of ARGs, achieving stable removal performance ranging from 1.0±0.4 to 2.8±0.7 log units, which is comparable to or higher than that reported for conventional activated sludge treatments. No reduction of the ARGs amount normalized to the total bacteria content (16S rDNA), was instead obtained, indicating that these genes are removed together with total bacteria and not specifically eliminated. Enhanced ARGs removal was obtained by sand filtration, while no reduction was achieved by both UV and PAA disinfection treatments tested in our study. Copyright © 2016 Elsevier B.V. All rights reserved.

Operational Lessons Learned During Bioreactor Demonstrations for Acid Rock Drainage Treatment

EPA Science Inventory

The U.S. Environmental Protection Agency’s Mine Waste Technology Program (MWTP) has emphasized the development of biologically-based treatment technologies for acid rock drainage (ARD). Progressively evolving technology demonstrations have resulted in significant advances in sul...

Concept Maps as Tools for Teaching.

ERIC Educational Resources Information Center

Moreira, Marco A.

1979-01-01

Discusses how concept maps with two dimensional diagrams which show hierarchical relationships among concepts of a discipline can be used in teaching physics. An example for teaching a course in electromagnetism at the Federal University of Rio Grande do Sul, Brazil is presented. (HM)

Redescription, lectotype designation and new records of Anastrepha luederwaldti Lima (Diptera, Tephritidae)

USDA-ARS?s Scientific Manuscript database

The previously poorly known species Anastrepha luederwaldti Lima, 1934 is redescribed based on a reexamination of the syntypes from São Paulo and additional specimens from Santa Catarina and Rio Grande do Sul, Brazil. A lectotype is designated....

Relationships between antibiotics and antibiotic resistance gene levels in municipal solid waste leachates in Shanghai, China.

PubMed

Wu, Dong; Huang, Zhiting; Yang, Kai; Graham, David; Xie, Bing

2015-04-07

Many studies have quantified antibiotics and antibiotic resistance gene (ARG) levels in soils, surface waters, and waste treatment plants (WTPs). However, similar work on municipal solid waste (MSW) landfill leachates is limited, which is concerning because antibiotics disposal is often in the MSW stream. Here we quantified 20 sulfonamide (SA), quinolone (FQ), tetracycline (TC), macrolide (ML), and chloramphenicol (CP) antibiotics, and six ARGs (sul1, sul2, tetQ, tetM, ermB, and mefA) in MSW leachates from two Shanghai transfer stations (TS; sites Hulin (HL) and Xupu (XP)) and one landfill reservoir (LR) in April and July 2014. Antibiotic levels were higher in TS than LR leachates (985 ± 1965 ng/L vs 345 ± 932 ng/L, n = 40), which was because of very high levels in the HL leachates (averaging at 1676 ± 5175 ng/L, n = 40). The mean MLs (3561 ± 8377 ng/L, n = 12), FQs (975 ± 1608 ng/L, n = 24), and SAs (402 ± 704 ng/L, n = 42) classes of antibiotics were highest across all samples. ARGs were detected in all leachate samples with normalized sul2 and ermB levels being especially elevated (-1.37 ± 1.2 and -1.76 ± 1.6 log (copies/16S-rDNA), respectively). However, ARG abundances did not correlate with detected antibiotic levels, except for tetW and tetQ with TC levels (r = 0.88 and 0.81, respectively). In contrast, most measured ARGs did significantly correlate with heavy metal levels (p < 0.05), especially with Cd and Cr. This study shows high levels of ARGs and antibiotics can prevail in MSW leachates and landfills may be an underappreciated as a source of antibiotics and ARGs to the environment.

Antimicrobial resistance and phylogenetic groups in isolates of Escherichia coli from seagulls at the Berlengas nature reserve.

PubMed

Radhouani, H; Poeta, P; Igrejas, G; Gonçalves, A; Vinué, L; Torres, C

2009-08-01

Fifty-three faecal samples from yellow-legged gulls (Larus cachinnans) at the Berlengas nature reserve in Portugal were cultured on Levine agar plates not supplemented with antimicrobial agents, and one Escherichia coli colony was isolated and identified from each sample. The percentages of resistant isolates for each of the drugs were ampicillin (43.4 per cent), tetracycline (39.6 per cent), nalidixic acid (34.0 per cent), streptomycin (32.1 per cent), trimethoprim-sulfamethoxazole (SXT) (26.4 per cent), ciprofloxacin (18.9 per cent), chloramphenicol (18.9 per cent), gentamicin (7.5 per cent), tobramycin (7.5 per cent) amikacin (5.7 per cent) and amoxicillin-clavulanic acid (1.9 per cent). All the isolates were susceptible to cefoxitin, ceftazidime, cefotaxime, aztreonam and imipenem. The following resistance genes were detected: bla(TEM) (17 of 23 ampicillin-resistant isolates), tet(A) and/or tet(B) (18 of 21 tetracycline-resistant isolates), aadA (12 of 17 streptomycin-resistant isolates), cmlA (all chloramphenicol-resistant isolates), aac(3)-II with or without aac(3)-IV (all four gentamicin-resistant isolates), and sul1 and/or sul2 and/or sul3 (all 14 SXT-resistant isolates). The intI1 gene was detected in 10 of 14 SXT-resistant isolates, and three of them also contained class 2 integrons; four different gene cassette arrangements were identified among class 1 integrons (aadA, dfrA1+aadA1, dfrA12+orfF+aadA2 and sat+psp+aadA2) and one among the class 2 integrons (dfrA1+sat+aadA1). Ninety per cent of the isolates were included in the A or B1 phylogenetic groups.

Serological and molecular inquiry of Chagas disease in an Afro-descendant settlement in Mato Grosso do Sul State, Brazil

PubMed Central

Martins, Mariana Furquim da Silva; Pereira, Mariane Barroso; Ferreira, Juliana de Jesus Guimarães; França, Adriana de Oliveira; Cominetti, Marlon Cézar; Ferreira, Eduardo de Castro; Dorval, Maria Elizabeth Moraes Cavalheiros; Rossi, Cláudio Lúcio; Mazon, Sílvia de Barros; de Almeida, Eros Antonio; Costa, Sandra Cecília Botelho; Marcon, Gláucia Elisete Barbosa

2018-01-01

Furnas do Dionísio is a Brazilian Afro-descendant settlement in the city of Jaraguari, 21.4 miles from Campo Grande, Mato Grosso do Sul, Brazil. Approximately 96 families live in this quilombola (Maroon) settlement, also known in Brazil as a remnant community of descendants of African slaves. Recent studies found 20% of households were infested by triatomines, 18% of insects captured in the community were infected by Trypanosoma cruzi, and 22.7% of dogs presented T. cruzi antibodies. The low prevalence of Chagas disease observed in humans in Mato Grosso do Sul State is attributed to its arrival via colonist migration and subsequent transplacental transmission. In order to gain a better understanding of the T. cruzi cycle in residents of the study community, serological and molecular tests were carried out to diagnose Chagas disease. In the present study, 175 residents between 2 and 80 years old were included. A total of 175 participants were interviewed and 170 provided blood samples, which were tested for T. cruzi antibodies with serological tests. Molecular diagnosis was performed in 167 participants by PCR (KDNA) and NPCR (satellite DNA) tests. One of the 170 samples tested positive for all serological tests performed. The overall frequency of Chagas disease in the community was low (0.6%). Interview responses revealed that 66.3% knew of triatomine insects and 65.7% reported having had no contact with them. Physical improvements to residences, together with vector surveillance and control by the State and municipal governments and local ecological conservation contribute to the low frequency of the Chagas disease in this quilombola community. PMID:29315305

Anxiolytic effects of a semipurified constituent of guaraná seeds on rats in the elevated T-maze test.

PubMed

Roncon, Camila Marroni; Biesdorf de Almeida, Camila; Klein, Traudi; de Mello, João Carlos Palazzo; Audi, Elisabeth Aparecida

2011-02-01

The objective of this study was to investigate the effects of chronic administration of a semi-purified extract (Purified Extract A--PEA; 4, 8, or 16 mg/kg) of PAULLINIA CUPANA (guaraná) seeds on rats submitted to the elevated T-maze (ETM) model of generalized anxiety and panic disorders. The selective serotonin (5-HT) reuptake inhibitor (SSRI) paroxetine (PAR; 3 mg/kg), was used as a positive control. To evaluate possible serotonergic and dopaminergic neurotransmission involvement in the action of PEA during the ETM test, ineffective doses of metergoline (MET; 5-HT (2A/2C) antagonist receptor) or sulpiride (SUL; dopaminergic receptor antagonist) were acutely administered together with the PEA. The locomotion of the rats was assessed in a circular arena following each drug treatment. Both PEA (8 and 16 mg/kg) and PAR (3 mg/kg) increased one-way escape latencies from the open arm of the ETM, indicating a panicolytic effect compared to the control group. MET, in higher doses (1, 2 or 3 mg/kg), produced a panicolytic effect in the ETM test, whereas SUL did not (10, 20 or 40 mg/kg). The panicolytic effect produced by PEA (8 mg/kg) was blocked by both MET (2 mg/kg) and SUL (20 mg/kg), whereas the panicolytic effect produced by PAR (3 mg/kg) was blocked only by MET (2 mg/kg). These results show that chronic treatment with PEA produces a panicolytic effect during the ETM test, and that the dopaminergic and the serotonergic neurotransmission systems are involved in this effect. © Georg Thieme Verlag KG Stuttgart · New York.

Characterization of antimicrobial resistance of Vibrio parahaemolyticus from cultured sea cucumbers (Apostichopus japonicas).

PubMed

Jiang, Y; Yao, L; Li, F; Tan, Z; Zhai, Y; Wang, L

2014-08-01

This study was aimed to evaluate the antimicrobial resistance and molecular resistance mechanisms of 87 Vibrio parahaemolyticus isolates from cultured sea cucumbers (Apostichopus japonicus). The results showed that all isolates were resistant to ampicillin and cephazolin, fewer of them were resistant to streptomycin (43·7%), cefuroxime sodium (18·4%), tetracycline (4·6%), sulphamethoxazole/trimethoprim (2·3%) and four quinolones (2·3%). More than half (56·2%) of the isolates displayed multiple resistance to at least three antimicrobials. The resistance genes were detected in all antimicrobial-resistant isolates except two tetracycline-resistant isolates. Among all these tested resistance genes, blaTEM , sul2, strA and strB were predominant, and none of blaSHV , blaCTX-M , blaOXA , sul1, sul3, tetA, tetM and tetQ genes was detected. Point mutations were found in quinolone resistance-determining regions of gyrA and parC genes in quinolone-resistant isolates. All isolates harboured class 1 integrons but only one carried gene cassette without any resistance genes, and none of them was positive to class 2, 3 integrons and SXT constins. These results indicate that the antimicrobial-resistant V. parahaemolyticus isolates from sea cucumbers and resistance genes could be potential risks to public health or other environments. This study is the first report on characterization of antimicrobial resistance of Vibrio parahaemolyticus from sea cucumbers (Apostichopus japonicus). Our findings reveal a high level of resistance to some antimicrobials and prevalence of the resistance genes in V. parahaemolyticus isolates from sea cucumbers and underline the need for prudent use of antimicrobials in aquaculture to minimize the spread of antimicrobial-resistant V. parahaemolyticus. © 2014 The Society for Applied Microbiology.

Evolutionary Relationships and Functional Diversity of Plant Sulfate Transporters

PubMed Central

Takahashi, Hideki; Buchner, Peter; Yoshimoto, Naoko; Hawkesford, Malcolm J.; Shiu, Shin-Han

2011-01-01

Sulfate is an essential nutrient cycled in nature. Ion transporters that specifically facilitate the transport of sulfate across the membranes are found ubiquitously in living organisms. The phylogenetic analysis of known sulfate transporters and their homologous proteins from eukaryotic organisms indicate two evolutionarily distinct groups of sulfate transport systems. One major group named Tribe 1 represents yeast and fungal SUL, plant SULTR, and animal SLC26 families. The evolutionary origin of SULTR family members in land plants and green algae is suggested to be common with yeast and fungal SUL and animal anion exchangers (SLC26). The lineage of plant SULTR family is expanded into four subfamilies (SULTR1–SULTR4) in land plant species. By contrast, the putative SULTR homologs from Chlorophyte green algae are in two separate lineages; one with the subfamily of plant tonoplast-localized sulfate transporters (SULTR4), and the other diverged before the appearance of lineages for SUL, SULTR, and SLC26. There also was a group of yet undefined members of putative sulfate transporters in yeast and fungi divergent from these major lineages in Tribe 1. The other distinct group is Tribe 2, primarily composed of animal sodium-dependent sulfate/carboxylate transporters (SLC13) and plant tonoplast-localized dicarboxylate transporters (TDT). The putative sulfur-sensing protein (SAC1) and SAC1-like transporters (SLT) of Chlorophyte green algae, bryophyte, and lycophyte show low degrees of sequence similarities with SLC13 and TDT. However, the phylogenetic relationship between SAC1/SLT and the other two families, SLC13 and TDT in Tribe 2, is not clearly supported. In addition, the SAC1/SLT family is absent in the angiosperm species analyzed. The present study suggests distinct evolutionary trajectories of sulfate transport systems for land plants and green algae. PMID:22629272

Evolutionary relationships and functional diversity of plant sulfate transporters.

PubMed

Takahashi, Hideki; Buchner, Peter; Yoshimoto, Naoko; Hawkesford, Malcolm J; Shiu, Shin-Han

2011-01-01

Sulfate is an essential nutrient cycled in nature. Ion transporters that specifically facilitate the transport of sulfate across the membranes are found ubiquitously in living organisms. The phylogenetic analysis of known sulfate transporters and their homologous proteins from eukaryotic organisms indicate two evolutionarily distinct groups of sulfate transport systems. One major group named Tribe 1 represents yeast and fungal SUL, plant SULTR, and animal SLC26 families. The evolutionary origin of SULTR family members in land plants and green algae is suggested to be common with yeast and fungal SUL and animal anion exchangers (SLC26). The lineage of plant SULTR family is expanded into four subfamilies (SULTR1-SULTR4) in land plant species. By contrast, the putative SULTR homologs from Chlorophyte green algae are in two separate lineages; one with the subfamily of plant tonoplast-localized sulfate transporters (SULTR4), and the other diverged before the appearance of lineages for SUL, SULTR, and SLC26. There also was a group of yet undefined members of putative sulfate transporters in yeast and fungi divergent from these major lineages in Tribe 1. The other distinct group is Tribe 2, primarily composed of animal sodium-dependent sulfate/carboxylate transporters (SLC13) and plant tonoplast-localized dicarboxylate transporters (TDT). The putative sulfur-sensing protein (SAC1) and SAC1-like transporters (SLT) of Chlorophyte green algae, bryophyte, and lycophyte show low degrees of sequence similarities with SLC13 and TDT. However, the phylogenetic relationship between SAC1/SLT and the other two families, SLC13 and TDT in Tribe 2, is not clearly supported. In addition, the SAC1/SLT family is absent in the angiosperm species analyzed. The present study suggests distinct evolutionary trajectories of sulfate transport systems for land plants and green algae.

Chlorine disinfection increases both intracellular and extracellular antibiotic resistance genes in a full-scale wastewater treatment plant.

PubMed

Liu, Shan-Shan; Qu, Hong-Mei; Yang, Dong; Hu, Hui; Liu, Wei-Li; Qiu, Zhi-Gang; Hou, Ai-Ming; Guo, Jianhua; Li, Jun-Wen; Shen, Zhi-Qiang; Jin, Min

2018-06-01

The emergence and spread of antibiotic resistance has posed a major threat to both human health and environmental ecosystem. Although the disinfection has been proved to be efficient to control the occurrence of pathogens, little effort is dedicated to revealing potential impacts of disinfection on transmission of antibiotic resistance genes (ARGs), particularly for free-living ARGs in final disinfected effluent of urban wastewater treatment plants (UWWTP). Here, we investigated the effects of chlorine disinfection on the occurrence and concentration of both extracellular ARGs (eARGs) and intracellular ARGs (iARGs) in a full-scale UWWTP over a year. We reported that the concentrations of both eARGs and iARGs would be increased by the disinfection with chlorine dioxide (ClO 2 ). Specifically, chlorination preferentially increased the abundances of eARGs against macrolide (ermB), tetracycline (tetA, tetB and tetC), sulfonamide (sul1, sul2 and sul3), β-lactam (ampC), aminoglycosides (aph(2')-Id), rifampicin (katG) and vancomycin (vanA) up to 3.8 folds. Similarly, the abundances of iARGs were also increased up to 7.8 folds after chlorination. In terms of correlation analyses, the abundance of Escherichia coli before chlorination showed a strong positive correlation with the total eARG concentration, while lower temperature and higher ammonium concentration were assumed to be associated with the concentration of iARGs. This study suggests the chlorine disinfection could increase the abundances of both iARGs and eARGs, thereby posing risk of the dissemination of antibiotic resistance in environments. Copyright © 2018 Elsevier Ltd. All rights reserved.

Noroviruses associated with outbreaks of acute gastroenteritis in the State of Rio Grande do Sul, Brazil, 2004-2011.

PubMed

de Andrade, Juliana da Silva Ribeiro; Rocha, Monica Simões; Carvalho-Costa, Felipe Aníbal; Fioretti, Julia Monassa; Xavier, Maria da Penha Trindade Pinheiro; Nunes, Zenaida Maria Alves; Cardoso, Jeanice; Fialho, Alexandre Madi; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira

2014-11-01

Acute gastroenteritis norovirus (NoV) in a country of continental dimensions like Brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. To demonstrate the role of NoV in outbreaks occurring in the State of Rio Grande do Sul, Southern Brazil, we determined its prevalence, as well as the genotypes associated, and evaluated clinical and epidemiological aspects. NoV investigation was carried out in rotavirus group A negative stool samples from 2265 patients from 741 outbreaks that occurred in the State of Rio Grande do Sul, Brazil, during a period of eight years (2004-2011). NoV detection and nucleotide sequencing for genotype characterization was carried by using sets of primers targeting a conservative Rd-Rp polymerase genome region and the viral capsid gene, respectively. NoVs were detected in 817 stool samples (36.1%) and associated with 327 outbreaks (44.1%). NoV GII.2, GII.3, GII.4, GII.6, GII.12, GII.13, GII.14, GII.15, GII.17, GII.21; and GI.1 and GI.3 were characterized. GII.4 was the most frequently detected (72.3%), with five variants identified (Asia_2003, Hunter_2004, Yerseke_2006a, Den_Haag_2006b, New Orleans_2009). This study describes the first detection of GI.1 and GII.13 and GII.15 in Brazil and demonstrates NoV winter-spring seasonality in this region of the country. NoVs were responsible for almost 50% of outbreaks, with about 70% of them resulting from genotype GII.4 and its variants. The seasonality observed could help health authorities to establish a system of active surveillance in order to reduce NoV impact especially in congregate settings. Copyright © 2014 Elsevier B.V. All rights reserved.

Epidemiologic situation of tuberculosis in Rio Grande do Sul: an analysis about Sinan's data between 2003 and 2012 focusing on indigenous peoples.

PubMed

Mendes, Anapaula Martins; Bastos, João Luiz; Bresan, Deise; Leite, Maurício Soares

2016-01-01

This article analyzes the epidemiological situation of tuberculosis in the state of Rio Grande do Sul, emphasizing the indigenous population. The data are based on the Information System of Grievance Notification (Sinan) between 2003 and 2012. The notified cases of tuberculosis were analyzed according to age, sex, zone of residence, input type, means of diagnosis, clinical form, anti-HIV exam, medical care, supervised treatment (in Portuguese, TDO), closure, and race. The highest incidence rates in the period were among Afro-Brazilians, yellow, and indigenous peoples. The cases affected mainly adult men living in urban areas. Indigenous peoples showed the highest rates of notifications among people aged less than 10 years (12%). In the sputum test, missing information and not-performed exams reached more than 50.0% in all periods and groups. The cure was more prevalent among white people (66.2%); indigenous, brown, and Afro-Brazilian people presented the lowest cure rates: 59.4, 58.4, and 60%, respectively. Tuberculosis is one of the biggest problems in Rio Grande do Sul. The actions of diagnosis, clinical form, and treatment of the cases have not been implemented as proposed. The indigenous peoples' situation is similar and diverse at the same time in comparison with other peoples from different areas of Brazil. Nevertheless, it is unfavorable on a balanced evaluation of the whole scenario. Furthermore, the discrepancies among races are evident: the indigenous and Afro-Brazilian peoples fill the spread sheet, in general terms, on the worst situation, whereas the white people fill the data with the best health situation.

Dissemination of antibiotic resistance genes and their potential removal by on-farm treatment processes in nine swine feedlots in Shandong Province, China.

PubMed

Ben, Weiwei; Wang, Jian; Pan, Xun; Qiang, Zhimin

2017-01-01

This work investigated the dissemination of antibiotic resistance genes (ARGs) encoding resistance to sulfonamide and tetracycline antibiotics in nine swine feedlots located in Shandong Province of China, and examined their potential removal by various on-farm treatment processes. Results indicate that the target ARGs were widely distributed in swine wastes, with mean relative abundances ranging from 3.3 × 10 -5 (tetC) to 5.2 × 10 -1 (tetO) in swine manure and from 7.3 × 10 -3 (tetC) to 1.7 × 10 -1 (tetO) in swine wastewater. The mean relative ARG abundances ranged from 9.9 × 10 -5 (tetW) to 1.1 × 10 -2 (tetO) in soils and from 3.1 × 10 -4 (tetW) to 1.1 × 10 -2 (sul2) in receiving river sediments, indicating that the farmland application of swine manure compost and the discharge of swine wastewater promoted the dissemination of ARGs into adjacent environments. Microbial fermentation bed (MFB) could reduce the relative ARG abundances by 0-1.18 logs. However, septic tank, biogas digester and natural drying methods were relatively ineffective for ARG removal, and the relative abundances of some ARGs (i.e., tetC, tetG, sul1, and sul2) even increased by 0.74-3.90 logs in treated wastes. Bacterial diversity analysis indicates that the evolution of bacterial communities in the MFB played a crucial role in eliminating the ARGs. This study helps the effective assessment and management of ecological risks arising from ARGs in swine feedlots. Copyright © 2016 Elsevier Ltd. All rights reserved.

Stability-Indicating HPTLC Method for Studying Stress Degradation Behavior of Sulbutiamine HCl

PubMed Central

Farid, Nehal F.; Abdelwahab, Nada S.

2016-01-01

Sulbutiamine (SUL) is an ester of thiazides with neurotropic action. A new stability indicating HPTLC method has been developed and validated for the determination of SUL in the presence of different degradation products. The drug was subjected to different stress conditions following ICH strategy such as hydrolytic degradation (neutral, alkaline and acidic hydrolysis), oxidation, photodegradation and dry heat degradation. The drug demonstrated degradation under all decomposition conditions except neutral hydrolysis and dry heat, where the drug was completely degraded with 0.1 N NaOH, 1 N HCl and 30% H2O2 while it was partially degradaed by 0.1 N HCl, 3% H2O2 and UV light. Structure elucidation of the resulting degradation products was performed using ESI-Q-MS–MS. A well-defined peak for SUL was obtained at Rf = 0.46 and was completely separated from all obtained degradation products. Chromatographic separation was carried out on HPTLC aluminum plates precoated with silica gel 60 F254 using acetone–methylene chloride–ammonia buffer (pH 8.5 ± 0.2) (7:3:0.5, v/v) as a developing system. Densitometric scanning of the separated peaks was performed at 254 nm. System suitability testing parameters were calculated to ascertain the quality performance of the developed method. The method was validated with respect to USP guidelines regarding accuracy, precision, specificity, robustness and ruggedness. Good correlation coefficients were achieved in the range of 0.4–5.0 µg/band, and the limit of detection and limit of quantitation were found to be 0.11 and 0.33 µg/band, respectively. The utility of the suggested method was verified by application to Arcalion forte® tablets where no interference from additives was found. PMID:26759487

Serological and molecular inquiry of Chagas disease in an Afro-descendant settlement in Mato Grosso do Sul State, Brazil.

PubMed

Martins, Mariana Furquim da Silva; Pereira, Mariane Barroso; Ferreira, Juliana de Jesus Guimarães; França, Adriana de Oliveira; Cominetti, Marlon Cézar; Ferreira, Eduardo de Castro; Dorval, Maria Elizabeth Moraes Cavalheiros; Rossi, Cláudio Lúcio; Mazon, Sílvia de Barros; de Almeida, Eros Antonio; Costa, Sandra Cecília Botelho; Marcon, Gláucia Elisete Barbosa

2018-01-01

Furnas do Dionísio is a Brazilian Afro-descendant settlement in the city of Jaraguari, 21.4 miles from Campo Grande, Mato Grosso do Sul, Brazil. Approximately 96 families live in this quilombola (Maroon) settlement, also known in Brazil as a remnant community of descendants of African slaves. Recent studies found 20% of households were infested by triatomines, 18% of insects captured in the community were infected by Trypanosoma cruzi, and 22.7% of dogs presented T. cruzi antibodies. The low prevalence of Chagas disease observed in humans in Mato Grosso do Sul State is attributed to its arrival via colonist migration and subsequent transplacental transmission. In order to gain a better understanding of the T. cruzi cycle in residents of the study community, serological and molecular tests were carried out to diagnose Chagas disease. In the present study, 175 residents between 2 and 80 years old were included. A total of 175 participants were interviewed and 170 provided blood samples, which were tested for T. cruzi antibodies with serological tests. Molecular diagnosis was performed in 167 participants by PCR (KDNA) and NPCR (satellite DNA) tests. One of the 170 samples tested positive for all serological tests performed. The overall frequency of Chagas disease in the community was low (0.6%). Interview responses revealed that 66.3% knew of triatomine insects and 65.7% reported having had no contact with them. Physical improvements to residences, together with vector surveillance and control by the State and municipal governments and local ecological conservation contribute to the low frequency of the Chagas disease in this quilombola community.

Occurrence and diversity of antibiotic resistance in untreated hospital wastewater.

PubMed

Wang, Qiang; Wang, Panliang; Yang, Qingxiang

2018-04-15

Antibiotics, antibiotic-resistant bacteria (ARB), antibiotic-resistance genes (ARGs), and mobile genetic elements (MGEs) have been reported in many environments. However, the investigation of their occurrence and diversity in untreated hospital wastewater is still insufficient. High concentrations of antibiotic residues were found in hospital wastewater using solid-phase extraction and UPLC-MS/MS analysis. The concentrations of six of 14 antibiotics reached μg/L levels in the hospital wastewater, which is higher than reported in other aquatic environments. Results of high-throughput sequencing analysis indicated that sequences affiliated to genera Escherichia and Acinetobacter were the predominant in the cultivable multiple-antibiotic-resistant bacteria (CMARB) recovered from the wastewater of three hospitals in China, with compositions of 34%-74%. Notably, several genera containing clinically pathogenic or opportunistic CMARB (e.g., Escherichia, Acinetobacter, Aeromonas, Myroides, Enterococcus, Proteus, Pseudomonas, and Streptococcus) were detected at high relative abundances in the wastewaters of the three hospitals. High-capacity quantitative PCR showed that 131-139 unique ARGs of the 178 targeted genes were detected in the hospital wastewaters. The high prevalence of five MGEs and 12 ARGs was confirmed with qPCR, and some positive correlations between ARGs and MGEs were identified, such as between intI1 and qnrD, intI2 and sul3, intI3 and tetX, Tn916/Tn1545 and sul2, and ISCR1 and sul3. These results suggest that highly abundant antibiotic-resistant pathogens and highly mobile ARGs already exist in the human body, and that their release from hospitals without effective treatment poses high risks to environments and human health. Copyright © 2017 Elsevier B.V. All rights reserved.

Antibiotic Resistance, Virulence, and Genetic Background of Community-Acquired Uropathogenic Escherichia coli from Algeria.

PubMed

Yahiaoui, Merzouk; Robin, Frédéric; Bakour, Rabah; Hamidi, Moufida; Bonnet, Richard; Messai, Yamina

2015-10-01

The aim of the study was to investigate antibiotic resistance mechanisms, virulence traits, and genetic background of 150 nonrepetitive community-acquired uropathogenic Escherichia coli (CA-UPEC) from Algeria. A rate of 46.7% of isolates was multidrug resistant. bla genes detected were blaTEM (96.8% of amoxicillin-resistant isolates), blaCTX-M-15 (4%), overexpressed blaAmpC (4%), blaSHV-2a, blaTEM-4, blaTEM-31, and blaTEM-35 (0.7%). All tetracycline-resistant isolates (51.3%) had tetA and/or tetB genes. Sulfonamides and trimethoprim resistance genes were sul2 (60.8%), sul1 (45.9%), sul3 (6.7%), dfrA14 (25.4%), dfrA1 (18.2%), dfrA12 (16.3%), and dfrA25 (5.4%). High-level fluoroquinolone resistance (22.7%) was mediated by mutations in gyrA (S83L-D87N) and parC (S80I-E84G/V or S80I) genes. qnrB5, qnrS1, and aac(6')-Ib-cr were rare (5.3%). Class 1 and/or class 2 integrons were detected (40.7%). Isolates belonged to phylogroups B2+D (50%), A+B1 (36%), and F+C+Clade I (13%). Most of D (72.2%) and 38.6% of B2 isolates were multidrug resistant; they belong to 14 different sequence types, including international successful ST131, ST73, and ST69, reported for the first time in the community in Algeria and new ST4494 and ST4529 described in this study. Besides multidrug resistance, B2 and D isolates possessed virulence factors of colonization, invasion, and long-term persistence. The study highlighted multidrug-resistant CA-UPEC with high virulence traits and an epidemic genetic background.

Evolution of Regions Containing Antibiotic Resistance Genes in FII-2-FIB-1 ColV-Colla Virulence Plasmids.

PubMed

Moran, Robert A; Hall, Ruth M

2018-05-01

Three ColV virulence plasmids carrying antibiotic resistance genes were assembled from draft genome sequences of commensal ST95, ST131, and ST2705 Escherichia coli isolates from healthy Australians. Plasmids pCERC4, pCERC5, and pCERC9 include almost identical backbones containing FII-2 and FIB-1 replicons and the conserved ColV virulence region with an additional ColIa determinant. Only pCERC5 includes a complete, uninterrupted F-like transfer region and was able to conjugate. pCERC5 and pCERC9 contain Tn1721, carrying the tet(A) tetracycline resistance determinant in the same location, with Tn2 (bla TEM ; ampicillin resistance) interrupting the Tn1721 in pCERC5. pCERC4 has a Tn1721/Tn21 hybrid transposon carrying dfrA5 (trimethoprim resistance) and sul1 (sulfamethoxazole resistance) in a class 1 integron. Four FII-2:FIB-1 ColV-ColIa plasmids in the GenBank nucleotide database have a related transposon in the same position, but an IS26 has reshaped the resistance gene region, deleting 2,069 bp of the integron 3'-CS, including sul1, and serving as a target for IS26 translocatable units containing bla TEM , sul2 and strAB (streptomycin resistance), or aphA1 (kanamycin/neomycin resistance). Another ColV-ColIa plasmid containing a related resistance gene region has lost the FII replicon and acquired a unique transfer region via recombination within the resistance region and at oriT. Eighteen further complete ColV plasmid sequences in GenBank contained FIB-1, but the FII replicons were of three types, FII-24, FII-18, and a variant of FII-36.

Telehealth in Rio Grande do Sul, Brazil: Bridging the Gaps.

PubMed

Harzheim, Erno; Gonçalves, Marcelo Rodrigues; Umpierre, Roberto Nunes; da Silva Siqueira, Ana Célia; Katz, Natan; Agostinho, Milena R; Oliveira, Elise B; Basso, Josué; Roman, Rudi; Dal Moro, Rafael G; Pilz, Carlos; Heinzelmann, Ricardo S; Schmitz, Carlos André Aita; Hauser, Lisiane; Mengue, Sotero Serrate

2016-11-01

In 1988, Brazil adopted a universal healthcare model in which access is mediated by the primary care level. However, difficulties have emerged in the coordination of care between the primary and specialized levels. Telehealth was thus proposed as a means to overcome this challenge. This article describes initiatives developed by a large Brazilian program, TelessaúdeRS/UFRGS, in the fields of teleconsultation, telediagnosis, tele-education, and information technology development to support the public healthcare system. TelessaúdeRS/UFRGS was established in 2010 to develop a telehealth platform and a support system for primary care teams with a special focus on optimizing the flow between primary and specialized levels of care. To define priorities, TelessaúdeRS analyzes the health needs of the Brazilian population and the most common inquiries it receives from primary care health professionals. This information is then combined with the best available scientific evidence for development of services. Since 2010, over 50,000 clinical consultations have been provided. More than 15,000 healthcare professionals have benefited from teleconsultations and from telediagnosis and tele-education activities. All services were provided using information technology solutions developed by the Telessaúde team, including smartphone apps and a Web-based National Telehealth Platform. The case of TelessaúdeRS/UFRGS shows that even in the presence of structural limitations, telemedicine is potentially useful to improve the quality of care and streamline the flow between different levels of care.

Translations on Environmental Quality, Number 140

DTIC Science & Technology

1977-07-05

the Hering Company of Blumenau, taking into consideration its needs for water for industrial reuse in future expansions. Other companies are also...River: completely dry. Lake Agua Preta - formed by the Doce River: completely dry. Lake Campello - formed by the Paraiba do Sul River; one of the

Giant-geode endowment of tumuli in the Veia Alta flow, Ametista do Sul

NASA Astrophysics Data System (ADS)

Hartmann, L. A.; Pertille, J.; Duarte, L. C.

2017-08-01

Tumuli are a common feature of pahoehoe basaltic flows, interspersed with pits, and furnished the necessary volume of rock in the Paraná volcanic province for hydrothermal alteration and ballooning to form large cavities (1-2 m common). Filling by amethyst and other minerals resulted in the largest world deposit of geodes, Ametista do Sul. The flat-lying fracture positioned 1 m below the 2-3 m thick geodic level crosses the plateau and is a major guide for exploration and gallery opening. The geodes are limited on the top by the platy joint layer, which is covered by an auto-breccia with undulating lower limit. This wave mimicks and is internal to the structure of tumuli and pits at the surface. This field-oriented survey of galleries selected out of 300 active mines resulted in the description of the internal structure of the remarkable Veia Alta pahoehoe flow, in addition to observations in Uruguay.

[Clinical-epidemiologic profile of paracoccidioidomycosis at the Stomatology Department of São Lucas Hospital, Pontificia Universidade Católica of Rio Grande do Sul].

PubMed

Verli, Flaviana Dornela; Marinho, Sandra Aparecida; Souza, Sílvio Correa de; Figueiredo, Maria Antonia Zancanaro de; Yurgel, Liliane Soares

2005-01-01

The aim of the article was to describe clinical and epidemiological features of 61 oral paracoccidioidomycosis cases from the Stomatology Department of São Lucas Hospital, analyzed from July 1976 to June 2004. The State of Rio Grande do Sul is an endemic region due to ecoepidemiologic conditions, that are favorable for the development of the yeast. Men are the most affected gender, with 58 (95%) affected compared to 3 (5%) women. Ii is most prevalent (70.5%) between 40 and 59 years of age. Agricultural labor was the most common occupation with 27/61 (44.3%) patients. Tobacco smoking was prevalent in 52 (85.3%) of the sample, and alcoholism was a frequent finding. All the patients showed stomatologic manifestations, with ulcers and mulberry-like stomatitis the most prevalent, these oral lesions were also observed in many anatomical sites. Alterations were present in 32 (65.3%) of the pulmonary radiographs.

[Managerial performance in public health services: a case study in Mato Grosso do Sul, Brazil].

PubMed

Barbieri, Ana Rita; Hortale, Virginia Alonso

2005-01-01

This paper presents part of a doctoral dissertation that developed a theoretical model capable of identifying managerial performance in various administrative levels of a Municipal Health Secretariat. The methodology was a case study of the Municipal Health Secretariat in Campo Grande, capital of the State of Mato Grosso do Sul, Brazil. The theoretical model was based on recent debates emphasizing the need to modernize public administration, with an emphasis on efficacy and efficiency in the organizations as a whole. Some 31 interviews were conducted with the objective of identifying the managers' performance, through questions based on their daily practices in planning, organization, direction, and control. Managers from higher hierarchical levels obtained better results, while those in basic health units generally developed activities and complied with decisions passed down by imposition, with limited capacity to plan, organize, or control activities pertaining to their management sphere. These results stem partially from the charismatic leadership and centralizing administration of the current management in the municipal health system.

[Strategic health planning based on determinants: case of the municipality of Campo Bom, Rio Grande do Sul State. A methodological proposal for the decentralized management].

PubMed

González, Martín Maximino León

2009-10-01

With the purpose to analyze the health strategic planning model based on determinants experienced in the municipality of Campo Bom, Rio Grande do Sul State, it was conducted an observational, qualitative study, of documental analysis as well as an evaluation of new process technologies in local health administration. This study contains an analysis of the methodological coherency and applicability of this model, based on the revision of the elaborated plans. The plans presented at Campo Bom case shows the possibility of integration and applicability at local level, of a health strategic planning model oriented to the new health concepts considering elements of different theoretical developments that enables the response to the most common local needs and situations. It was identified evolutional stages of health planning and analyzed integrative elements of the model and limitations of its application, pointing to the need of support the deepening on the study and the development of the field.

Multidrug-resistant Commensal Escherichia coli in Children, Peru and Bolivia

PubMed Central

Pallecchi, Lucia; Benedetti, Marta; Fernandez, Connie; Vallejos, Yolanda; Guzman, Elisa; Villagran, Ana Liz; Mantella, Antonia; Lucchetti, Chiara; Bartalesi, Filippo; Strohmeyer, Marianne; Bechini, Angela; Gamboa, Herlan; Rodríguez, Hugo; Falkenberg, Torkel; Kronvall, Göran; Gotuzzo, Eduardo; Paradisi, Franco; Rossolini, Gian Maria

2006-01-01

Using a rapid screening method, we investigated the prevalence of fecal carriage of antimicrobial drug–resistant Escherichia coli in 3,174 healthy children from 4 urban settings in Peru and Bolivia. High resistance rates were observed for ampicillin (95%), trimethoprim-sulfamethoxazole (94%), tetracycline (93%), streptomycin (82%), and chloramphenicol (70%). Lower resistance rates were observed for nalidixic acid (35%), kanamycin (28%), gentamicin (21%), and ciprofloxacin (18%); resistance to ceftriaxone and amikacin was uncommon (<0.5%). In a random sample of 1,080 resistant E. coli isolates, 90% exhibited a multidrug-resistance (MDR) phenotype. The 2 most common MDR phenotypes (ampicillin/tetracycline/trimethoprim-sulfamethoxazole and ampicillin/tetracycline/trimethoprim-sulfamethoxazole/chloramphenicol) could be transferred en bloc in conjugation experiments. The most common acquired resistance genes were blaTEM, tet(A), tet(B), drfA8, sul1, sul2, and catI. These findings underscore the magnitude of the problem of antimicrobial drug resistance in low-resource settings and the urgent need for surveillance and control of this phenomenon. PMID:16707045

[Frequency of healthy eating habits measured by the 10 Steps to Healthy Eating score proposed by the Ministry of Health: Pelotas, Rio Grande do Sul State, Brazil].

PubMed

Vinholes, Daniele Botelho; Assunção, Maria Cecília Formoso; Neutzling, Marilda Borges

2009-04-01

This study aimed to measure frequency of healthy eating habits and associated factors using the 10 Steps to Healthy Eating score proposed by the Ministry of Health in the adult population in Pelotas, Rio Grande do Sul State, Brazil. A cross-sectional population-based survey was conducted on a cluster sample of 3,136 adult residents in Pelotas. The frequency of each step to healthy eating was collected with a pre-coded questionnaire. Data analysis consisted of descriptive analysis, followed by bivariate analysis using the chi-square test. Only 1.1% of the population followed all the recommended steps. The average number of steps was six. Step four, salt intake, showed the highest frequency, while step nine, physical activity, showed the lowest. Knowledge of the population's eating habits and their distribution according to demographic and socioeconomic variables is important to guide local and national strategies to promote healthy eating habits and thus improve quality of life.

Public health agendas addressing violence against rural women - an analysis of local level health services in the State of Rio Grande do Sul, Brazil.

PubMed

da Costa, Marta Cocco; Lopes, Marta Julia Marques; Soares, Joannie dos Santos Fachinelli

2015-05-01

This study analyses health managers' perceptions of local public health agendas addressing violence against rural women in municipalities in the southern part of the State Rio Grande do Sul in Brazil. It consists of an exploratory descriptive study utilizing a qualitative approach. Municipal health managers responsible for planning actions directed at women's health and primary health care were interviewed. The analysis sought to explore elements of programmatic vulnerability related to violence in the interviewees' narratives based on the following dimensions of programmatic vulnerability: expression of commitment, transformation of commitment into action, and planning and coordination. It was found that local health agendas directed at violence against rural women do not exist. Health managers are therefore faced with the challenge of defining lines of action in accordance with the guidelines and principles of the SUS. The repercussions of this situation are expressed in fragile comprehensive services for these women and programmatic vulnerability.

Multibiomarker approach in fish to assess the impact of pollution in a large Brazilian river, Paraiba do Sul.

PubMed

Linde-Arias, Ana Rosa; Inácio, Alan F; Novo, Leonardo A; de Alburquerque, Carla; Moreira, Josino C

2008-12-01

This article examines the advantages of the use of biomarkers as environmental indicators by applying it to Paraiba do Sul watershed, one of the most important Brazilian water bodies, which is in a critical environmental situation. We use a multibiomarker approach in fish as an integrated strategy to assess the impact of pollution. It comprehends a general biomarker of fish health, the condition factor (CF), and specific biomarkers of contaminant exposure such as metallothionein (MT), acetylcholinesterase (AChE) activity and biliary polycyclic aromatic hydrocarbons (PAH) metabolites. Our results revealed different effects in the fish from diverse locations with varying degrees of pollution. Furthermore, fish located just upstream of the water-treatment plant of the metropolitan area of Rio de Janeiro has shown to be affected by metals. This study indicates the usefulness of integrating a set of biomarkers to define the effects of anthropogenic inputs in aquatic bodies under complex polluted situations.

A preliminary index of biotic integrity for monitoring the condition of the Rio Paraiba do Sul, southeast Brazil.

PubMed

Araujo, Francisco Gerson; Fichberg, Ilana; Pinto, Benjamin Carvalho Teixeira; Peixoto, Magna Galvao

2003-10-01

The biodiversity of many Brazilian rivers is seriously threatened by industrial and municipal pollution, and Rio Paraiba do Sul, located between two major industrial centers is one example of this situation. A survey of the fish assemblage was conducted from October 1998 to September 1999 and the data were used to develop an index of biotic integrity (IBI). We sampled three zones in bracketing a large urban-industrial complex to evaluate water quality changes and the usefulness of the IBI as a monitoring tool. Water quality was classified as poor upstream of the effluent discharges, very poor near the discharges, and poor-fair downstream of the discharges, with this latter situation revealing the current biological capacity of the river. Physical and chemical habitat characteristics were also measured at each site to construct an independent environmental index to validate the IBI. The habitat and IBI indices were highly correlated, suggesting this IBI would be applicable to other large rivers in southeast Brazil.

Polychlorinated biphenyls in umbilical cord serum of newborns from Rio Grande do Sul state, Brazil.

PubMed

Mohr, Susana; Sifuentes dos Santos, Joice; Schwanz, Thiago Guilherme; Wagner, Roger; Mozzaquatro, Joseane Oliveira; Lorenzoni, Alessandra Scherer; Costabeber, Ijoni Hilda

2015-12-07

Polychlorinated biphenyls (PCBs) are food-chain contaminants that have been shown to contaminate foods worldwide. The newborn are exposed to these organochlorine compounds across the placenta and through breastfeeding. They are proven to be carcinogenic and may contribute to congenital malformation etiology. This study examined levels of five PCB congeners (28, 52, 138, 153 and 180) in umbilical cord serum samples from 148 newborns from Rio Grande do Sul state, Brazil. Serum concentrations of PCBs were analyzed by gas chromatography with electron capture detection and mass spectrometry. Levels of ∑PCBs ranged from 0.35 to 55.17 ng/ml in umbilical cord serum positive samples, and PCB 138 was the most prevalent congener. Only 7.4% of samples presented no PCB congener. Some PCB congener cord serum levels were related to the locale of the mothers' residence, smoking and drinking habits, fruit consumption, and congenital malformation. Copyright © 2015 Elsevier B.V. All rights reserved.

[Femicide in Porto Alegre, Rio Grande do Sul State, Brazil: gender iniquities in dying].

PubMed

Meneghel, Stela Nazareth; Margarites, Ane Freitas

2017-12-18

Femicide is the murder of women as the result of gender inequalities. It is the most extreme form of violence against women. The theoretical and methodological frame of reference used in this study was patriarchy theory and critical discourse analysis. We analyzed the discourses from 64 police inquiries categorized as femicides in Porto Alegre, Rio Grande do Sul State, Brazil, from 2006 to 2010. The victims were mostly poor young women living in outlying areas of the city with high rates of prostitution and women murdered by the drug traffic, deaths not routinely classified as femicides by the police. Many inquiries were shelved for purported lack of evidence, and many other cases were not even started. The discourse in the police reports often demeaned and blamed the victims, although some criticized the inequalities between men and women and identified the lethal effects of male chauvinism. Police inquiries are important sources for studying femicide in society, adding abundant information on the crimes' victims, perpetrators, and scenarios.

Identification of priority Health Regions in the State of Rio Grande do Sul, Brazil, for Health Surveillance actions.

PubMed

Mondini, Renata Petzhold; Menegolla, Ivone Andreatta; Silva, Eduardo Viegas da

2017-10-01

This paper aimed to identify the priority Health Regions of Rio Grande do Sul (RS) to implement Health Surveillance strengthening actions. This is a descriptive study with data from time series of 11 (eleven) Health Surveillance indicators of the Ministry of Health's 2016 Guidelines, Objectives, Targets and Indicators Journal agreed by the Bipartite Interagency Committee/RS. The selected indicators are synthesized to produce a Composite Health Surveillance Assessment Indicator (ICAVES) for each of the 30 Health Regions of the state, creating values ranging from zero (worst) to 1 (best), using the Human Development Index (HDI) construction calculation method. The lowest rates of the composite indicator are found in the Health Regions "20-Rota da Produção" and "19-Região do Botucaraí". These two Health Regions are priorities for the strengthening of collective Health Surveillance actions and the management of health risks and diseases, considering horizontal equity as guideline of the Unified Health System.

[Environmental, social, and roadway vulnerability in accidents involving transportation of hazardous products: a case study of the BR-101 highway between Osório and Torres in Rio Grande do Sul State, Brazil].

PubMed

Tinoco, Maria Auxiliadora Cannarozzo; Nodari, Christine Tessele; Pereira, Kimberllyn Rosa da Silva

2016-09-19

This study aimed to assess the environmental and social vulnerability and identify critical highway stretches for accidents involving transportation of hazardous products on the BR-101 highway between the cities of Osório and Torres in Rio Grande do Sul State, Brazil. The study's approach consisted of a multiple-criteria analysis combining highway safety analysis and environmental and social vulnerability analysis in the occurrence of accidents with hazardous products, plus cartographic analysis techniques. Thirty-eight kilometers of the highway showed high vulnerability, of which 8 kilometers with critical vulnerability, associated with bridges over rivers, water uptake points, a tunnel, environmental preservation areas, and an urban area. These stretches should be prioritized when developing action plans for accident mitigation and development of public policies for this highway. This proved to be an unprecedented approach when compared to existing studies and a potentially useful tool for decision-making in emergency operations.

Bryozoans from rio grande do sul continental shelf, southern Brazil.

PubMed

Ramalho, Laís V; Calliari, Lauro

2015-05-06

The continental shelf of Rio Grande do Sul (RS) is predominantly composed of unconsolidated sediments with a few hard substrates represented principally by beachrock. In this area there are elongate deposits of shell gravel material which are interpreted as indicators of the palaeo-shorelines. These Pleistocene deposits are overlapped by Holocene sediments (Recent), but are exposed during erosive events caused by extra-tropical cyclones, which provide the mixture of both sediments mainly during autumn and winter. The few studies on bryozoans made in this area previously recorded seven species, one fossil and the other six from Recent fluvial and marine environments. The aim of the present study was to describe the eight most abundant bryozoan species that occur in the inner RS shelf. Of these, four are new records for RS State (Arachnopusia aff. pusae, Hippomonavella brasiliensis, Turbicellepora pourtalesi, and Lifuella gorgonensis), and the other four are new to science (Chaperia taylori, Micropora nodimagna, Cellaria riograndensis, and Exochella moyani).

Comparative study of the floral biology and of the response of productivity to insect visitation in two rapeseed cultivars (Brassica napus L.) in Rio Grande do Sul.

PubMed

Blochtein, B; Nunes-Silva, P; Halinski, R; Lopes, L A; Witter, S

2014-11-01

Planning the artificial pollination of agricultural crops requires knowledge of the floral biology and reproductive system of the crop in question. Many studies have shown that rapeseed (Brassica napus Linnaeus) is self-compatible and self-pollinated, but its productivity may be increased by insect visitation. In the present study, the floral biology and the response of productivity to insect visitation of two rapeseed cultivars (Hyola 420 and Hyola 61) were analyzed and compared in three regions of Rio Grande do Sul, Brazil. The rapeseed flowers presented three stages during anthesis, with the time periods varying between the cultivars. Both cultivars are self-compatible, but free visitation of insects increased productivity by 17% in the Hyola 420 cultivar and by approximately 30% in the Hyola 61 cultivar. Therefore, it is concluded that the cultivar Hyola 61 is more dependent on insect pollination than Hyola 420.

[Regional health systems management: a case study in Rio Grande do Sul, Brazil].

PubMed

Lima, Juliano de Carvalho; Rivera, Francisco Javier Uribe

2006-10-01

This article analyzes the management system in a health district in the State of Rio Grande do Sul, Brazil, through qualitative analysis, using a case study as the methodology and macro-organization theory as the analytical framework. For the current management system in the 6th Health Region, a clear mission statement and wide acceptance by health workers are facilitating factors for the current organizational practices within the health system. Nevertheless, the way health coordinators are currently prioritizing their time has diverted necessary resources from critical problems towards more remedial issues. The 6th Health Region has encouraged social control (or public oversight) in order to improve accountability. However, there is room for improvement in quality assurance management, since there were no well-defined goals, objectives, or accountability. Decentralized consultancy provided to the municipalities and the funding model itself have both promoted decentralization and autonomy, although the strategy requires better regional integration and greater commitment in managerial practices.

[Mental health actions in the north of the state of Rio Grande do Sul, Brazil].

PubMed

Martins, Ricardo Vianna; Rossettob, Maíra; Sartori, Queli Daiane Nogueira; Pinto, Ader Campos; Van Der Sand, Isabel Cristina Pacheco; Hildebrandt, Leila Mariza

2012-03-01

The objective this study is to describe the actions and intervention projects in mental health in the health services of the cities that are part of Rio Grande do Sul's 15th Regional Health Coordinating Body (15a Coordenadoria Regional de Saúde). This is an exploratory, descriptive and qualitative research. A questionnaire was applied to health professionals and managers who were part of regional mental health forums. From the content analysis as proposed by Minayo, the following categories emerged- one which describes mental health care in the health services of the municipalities, including aspects related to mental disorders, to the organization of the mental health team, to the main developed actions and to the difficulties found; the other category is about intervention projects in mental health that, according to the subjects, will be implanted in the cities. Relevant points the regional network of mental health care were identified which will be helpful in planning and improving care.

Mental health care for Brazilian juvenile offenders.

PubMed

Costa, Nilson do Rosário; Silva, Paulo Roberto Fagundes da

2017-05-01

This paper analyzes the use of psychoactive drugs by juvenile offenders in Brazil in socio-educational facilities (USEs). It describes the guidelines of the national public policy and the quality of mental healthcare coordination by subnational public governments. This work draws on the hypothesis that USEs vertical governance is associated with the use of psychoactive medication. This is comparative study of two cases in Rio Grande do Sul and Minas Gerais. Data resulted from a sample of medical records and interviews with key informants. The paper shows that vertical governance observed in Rio Grande do Sul is directly associated with high prevalence of mental health disorder diagnosis, use of psychoactive medication and psychiatric medicalization by juvenile offenders deprived of liberty. These findings indicate that sanctions of imprisonment for illegal acts are producing a set of medicalization decisions that undermine juveniles' health rights. The national mental health policy guidelines encourage cautious decisions. Psychotherapies and rehabilitation actions are the advocated first-line interventions. The poor management of the psychopharmacological intervention favors multiple prescriptions.

Sulthiame but not levetiracetam exerts neurotoxic effect in the developing rat brain.

PubMed

Manthey, Daniela; Asimiadou, Stella; Stefovska, Vanya; Kaindl, Angela M; Fassbender, Jessica; Ikonomidou, Chrysanthy; Bittigau, Petra

2005-06-01

Antiepileptic drugs (AEDs) used to treat seizures in pregnant women, infants, and young children can cause cognitive impairment. One mechanism implicated in the development of neurocognitive deficits is a pathologic enhancement of physiologically occurring apoptotic neuronal death in the developing brain. We investigated whether the newer antiepileptic drug levetiracetam (LEV) and the older antiepileptic drug sulthiame (SUL) have neurotoxic properties in the developing rat brain. SUL significantly enhanced neuronal death in the brains of rat pups ages 0 to 7 days at doses of 100 mg/kg and above, whereas LEV did not show this neurotoxic effect. Dosages of both drugs used in the context of this study comply with an effective anticonvulsant dose range applied in rodent seizure models. Thus, LEV is an AED which lacks neurotoxicity in the developing rat brain and should be considered in the treatment of epilepsy in pregnant women, infants, and toddlers once general safety issues have been properly addressed.

Epidemiological study on leishmaniasis in an area of environmental tourism and ecotourism, State of Mato Grosso do Sul, 2006-2007.

PubMed

Andrade, Ana Rachel Oliveira de; Nunes, Vânia Lúcia Brandão; Galati, Eunice Aparecida Bianchi; de Arruda, Carla Cardozo Pinto; Santos, Mirella Ferreira da Cunha; Rocca, Maria Elizabeth Gizi; Aquino, Ricardo Braga

2009-01-01

The aims of this study were to carry out a serological survey of canine leishmaniasis and identify the phlebotomine fauna in the urban area of Bonito, Mato Grosso do Sul. The serological survey was conducted on a sample of 303 dogs, by means of the indirect immunofluorescence test. Phlebotomines were captured using automated light traps. The serological survey found that 30% of the dogs were seropositive, both from the center and from all districts of the town. A total of 2,772 specimens of phlebotomines were caught and the species most found was Lutzomyia longipalpis (90.4%), which corroborated its role as the vector of for canine visceral leishmaniasis in the region. Phlebotomines of the species Bichromomyia flaviscutellata (the main vector for Leishmania (Leishmania) amazonensis) and Nyssomyia whitmani (the vector for Leishmania (Viannia) brasiliensis) were also caught. The findings indicate the need for continuous epidemiological surveillance, with attention towards diminishing the vector breeding sites and the transmission of these diseases in that region.

Identification of an emergent bacterial blight of garlic in Brazil

USDA-ARS?s Scientific Manuscript database

Outbreaks of a bacterial blight disease occurred on garlic (Allium sativum) cultivars Roxo Caxiense, Quiteria and Cacador in Southern Brazil, and threatened the main production regions of Rio Grande do Sul State. Symptoms were characterized by watersoaked reddish streaks along the leaf midrib, follo...

40 CFR 721.1550 - Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1).

Code of Federal Regulations, 2014 CFR

2014-07-01

...). In addition, the following human health hazard statement shall appear on each label as specified at... they are true and do not alter the meaning of the required statement. Human health hazard statements...

40 CFR 721.1550 - Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1).

Code of Federal Regulations, 2012 CFR

2012-07-01

...). In addition, the following human health hazard statement shall appear on each label as specified at... they are true and do not alter the meaning of the required statement. Human health hazard statements...

Executive Summary Report - FY80 Geotechnical Siting Investigations.

DTIC Science & Technology

1980-11-30

MX siting investigation, geotech- nical summary, prime characterization sites, Rio Grande /Highlands Candidate Siting Province report. TR-26d , 19781...s * Subsurface projectlro of to sul rock limits ab ser i Oelneation of ro-k f o- Geologic high (-7000 !ps -) w- we velocities -Obta r Borings

Leinendera achaeta sp. n., a new species of robber fly from Brazil (Diptera, Asilidae, Asilinae).

PubMed

Camargo, Alexssandro; Vieira, Rodrigo; Köhler, Andreas; Rafael, José Albertino

2016-01-01

The third species of the Neotropical genus Leinendera Carrera, 1945, Leinendera achaeta sp. n., is described from Rio Grande do Sul state, Brazil. The habitus, wing and male terminalia are described and illustrated, and a key to the three Brazilian species is provided.

UCS-PROMOVE: The Engineer of the Future

ERIC Educational Resources Information Center

Villas-Boas, V.

2010-01-01

The Universidade de Caxias do Sul (UCS) elaborated the cooperative project called "The engineer of the future", with the objective of promoting science and engineering among high school teachers and students. This project aims to improve the quality of the teaching and to increase the interest of students in technological areas, leading…

Nonformal Continuing Education in Rural Brazil.

ERIC Educational Resources Information Center

Chesterfield, Ray; Schutz, Paulo

1978-01-01

The authors describe a nonformal adult vocational education program developed in Rio Grande do Sul, a rural Brazilian state, which offers in-school vocational education as well as adult continuing education. Truck trailers with vocational equipment and trained personnel visit rural communities to provide learning experiences both in and out of the…

Rice Water use efficiency and yield under continuous and intermittent irrigation

USDA-ARS?s Scientific Manuscript database

In the Brazilian state of Rio Grande do Sul, rice (Oryza sativa L.) is predominantly grown using continuous fl ood irrigation, which requires large quantities of fresh water. Due to increasing scarcity and demand for water, modern agricultural systems need to produce more food with less water. Th e ...

Developing Student Critical Thinking Skills through Teaching Psychology: An Interview with Claudio S. Hutz.

ERIC Educational Resources Information Center

McCarthy-Tucker, Sherri

2001-01-01

Presents an interview with Claudio S. Hutz, who is dean of Instituto de Psicologia at Universidade Federal do Rio Grande do Sul in Porto Alegre, Brazil, where he has been teaching psychology since 1977. Discusses topics such as teaching psychology in Brazil and developing critical thinking skills. (CMK)

Antibiotic resistance genes persist longer in soils with subsurface banded poultry litter

USDA-ARS?s Scientific Manuscript database

The objective of this study was to determine the concentration of AR genes for sulfonamide (sulI), tetracycline (tetW), streptomycin (strpB) and for the class one integrase (intI1) gene in soils with subsurface banded PL. Field scale plots were established with triplicate treatments of either no fer...

Vowel Harmony: A Variable Rule in Brazilian Portuguese.

ERIC Educational Resources Information Center

Bisol, Leda

1989-01-01

Examines vowel harmony in the "Gaucho dialect" of the Brazilian state of Rio Grande do Sul. Informants from four areas of the state were studied: the capital city (Porto Alegre), the border region with Uruguay, and two areas of the interior populated by descendants of nineteenth-century immigrants from Europe, mainly Germans and…

Communicating with Low-Income and Low Education Farmers in a Developing Country.

ERIC Educational Resources Information Center

Fett, John H.

Selected findings from a number of communication research projects conducted by, or in collaboration with, the Economic Studies and Research Institute (IEPE), Federal University of Rio Grande do Sul, Brazil, are reported. Communication research in IEPE during the past 4 years has concentrated on mass media performance and use, because extension…

Determinants of Educational Performance in Southern Brazil.

ERIC Educational Resources Information Center

Hansen, David O.

The study examined the effects of socioeconomic origin (SES) and mental ability (MA) on levels of educational (EA) and occupational aspiration (OA), academic performance (AP), and significant others' influence (SOI) for 1,950 5th grade elementary and 4th year gymnastic students in Rio Grande do Sul, Brazil. Representative samples of approximately…

SEASONAL VARIATION OF THE PARTICLE SIZE DISTRIBUTION OF POLYCYCLIC AROMATIC HYDROCARBONS AND OF MAJOR AEROSOL SPECIES IN CLAREMONT, CALIFORNIA. (R827352C020)

EPA Science Inventory

As part of the Southern California Particle Center and Supersite (SCPCS) activities, we measured, during all seasons, particle size distributions of 12 priority pollutant polycyclic aromatic hydrocarbons (PAHs), concurrently with elemental carbon (EC), organic carbon (OC), sul...

Detection of Sarcocystis felis-like protozoon in South American wild felids from Brazil

USDA-ARS?s Scientific Manuscript database

Wild felids are thought to share parasites with domestic cats. However, little is known of the coccidian parasites of wild felids. Weinvestigated the presence of Sarcocystis spp. in tissues of six species of Neotropical small felids killed in road accidents in the state of Rio Grande do Sul, Brazil ...

Non-Linear Stability of Magnetically Focused Particle Beams

DTIC Science & Technology

2008-11-30

PROJECT NUMBER N/A 5e. TASK NUMBER N/A 5f. WORK UNIT NUMBER N/A 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Instituto de Fisica ...Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39.18 Felipe Barbedo Rizzato Institute de Fisica , Universidade Federal do Rio Grande do Sul

Influence of zeolite and superphosphate as additives on antibiotic resistance genes and bacterial communities during factory-scale chicken manure composting.

PubMed

Peng, Shuang; Li, Huijie; Song, Dan; Lin, Xiangui; Wang, Yiming

2018-04-30

Factory-scale chicken manure composting added with zeolite (F), superphosphate (G), or zeolite and ferrous sulfate (FL) simultaneously, were evaluate for their effects on the behaviors of antibiotic resistance genes (ARGs) and bacterial communities. After composting, ARGs in manure decreased by 67.3% in the control, whereas the reductions were 86.5%, 68.6% and 72.2% in F, G and FL, respectively. ARGs encoding ribosomal protection proteins (tetO, tetB(P), and tetM) were reduced to a greater extent than tetG, tetL, sul1 and sul2. Bacteria pathogens were also effectively removed by composting. Network analysis showed that Firmicutes were the important potential host bacteria for ARGs. The bacterial communities and environmental factors, as well as the intI gene, contributed significantly to the variation of ARGs. The ARGs and integrons were reduced more when zeolite was added than when superphosphate was added; thus, it may be useful for reducing the risks of ARGs in chicken manure. Copyright © 2018 Elsevier Ltd. All rights reserved.

The ichthyofauna of limnic systems in Quaternary deposits of extreme southern Brazil

PubMed Central

Assumpção, Cindy M.; Quintela, Fernando M.; Corrêa, Fabiano; Loebmann, Daniel

2016-01-01

Abstract The Quaternary in the state of Rio Grande do Sul (RS), southern Brazil, is geologically represented by the coastal plain and was originated by successive events of Pleistocene-Holocene marine transgressions and the occurrence of alluvial deposits. This paper aimed to characterize the fish assemblage occurring in a swampy Quaternary area adjacent to Lagoa Pequena, a lacustrine system connected to the west margin of the Laguna dos Patos estuary. A checklist is also provided of the ichthyofauna so far recorded in limnic systems of Quaternary deposits in the state of Rio Grande do Sul. A total of 42 species was recorded, distributed in nine orders, 18 families and 31 genera. Characidae and Cichlidae were the most representative families, comprising 15 and 4 species respectively. A bibliographic revision associated to our sample data revealed the occurrence of 156 species in limnic systems inserted in RS Quaternary deposits (114 limnic, 15 marine/estuarine/limnic, ten marine/estuarine, nine estuarine/limnic and eight marine). Characiformes and Siluriformes are the most diverse orders, corroborating the Neotropical pattern. Seven species can be considered endemic to RS Quaternary deposits. PMID:28174498

Evaluating the effects of activated carbon on methane generation and the fate of antibiotic resistant genes and class I integrons during anaerobic digestion of solid organic wastes.

PubMed

Zhang, Jingxin; Mao, Feijian; Loh, Kai-Chee; Gin, Karina Yew-Hoong; Dai, Yanjun; Tong, Yen Wah

2018-02-01

The effects of activated carbon (AC) on methane production and the fate of antibiotic resistance genes (ARGs) were evaluated through comparing the anaerobic digestion performance and transformation of ARGs among anaerobic mono-digestion of food waste, co-digestion of food waste and chicken manure, and co-digestion of food waste and waste activated sludge. Results showed that adding AC in anaerobic digesters improved methane yield by at least double through the enrichment of bacteria and archaea. Conventional digestion process showed ability in removing certain types of ARGs, such as tetA, tetX, sul1, sul2, cmlA, floR, and intl1. Supplementing AC in anaerobic digester enhanced the removal of most of the ARGs in mono-digestion of food waste. The effects tended to be minimal in co-digestion of co-substrates such as chicken manure and waste activated sludge, both of which contain a certain amount of antibiotics. Copyright © 2017 Elsevier Ltd. All rights reserved.

Occurrence of 15 Haplotypes of Linepithema micans (Hymenoptera: Formicidae) in Southern Brazil.

PubMed

Ramalho, Manuela Oliveira; Martins, C; Campos, T; Nondillo, A; Botton, M; Bueno, O C

2017-08-01

The ant genus Linepithema is widely known, thanks to the pest species Linepithema humile (Mayr), which is easily mistaken for Linepithema micans (Forel) due to their morphological similarity. Like L. humile, L. micans is associated to the main grapevine pest in Brazil, Eurhizococcus brasiliensis (Wille), also known as ground pearl. Therefore, the present study uses mtDNA fragments to expand the knowledge of haplotype diversity and distribution of L. micans in the state of Rio Grande do Sul (Brazil), to understand the genetic differences of the populations identified in this study. We identified 15 haplotypes of L. micans spread across different localities. Twelve of these haplotypes were new for the species. The high haplotype diversity uncovered in Rio Grande do Sul (Brazil) for this species was predictable, as L. micans is in its native environment. Additional studies that take gene flow into account may reveal interesting aspects of diversity in these populations. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

[Pulmonary tuberculosis among residents of municipalities in Mato Grosso do Sul State, Brazil, bordering on Paraguay and Bolivia].

PubMed

Marques, Marli; Ruffino-Netto, Antonio; Marques, Ana Maria Campos; Andrade, Sonia Maria Oliveira de; Silva, Baldomero Antonio Kato da; Pontes, Elenir Rose Jardim Cury

2014-12-01

This study analyzed the epidemiological profile of pulmonary tuberculosis from 2007 to 2010 in municipalities (counties) in Mato Grosso do Sul State, Brazil, that border on Paraguay and Bolivia. In the border region, the incidence rate (49.1/100,000 inhabitants), mortality rate (4.0/100,000 inhabitants), and treatment dropout rate (11.3%) were 1.6, 1.8, and 1.5 times higher than in the non-border region. Among indigenous individuals in the border region, the rates for incidence (253.4/100,000 inhabitants), mortality (11.6/100,000 inhabitants), and HIV/TB co-infection (1.9/100,000 inhabitants) were 6.4, 3.2, and 1.9 times higher than in non-indigenous individuals in this region. Living in the border regions was inversely associated with HIV/TB co-infection. Indigenous ethnicity was associated statistically with not abandoning TB treatment. The study concludes that the population residing in these municipalities along the border is exposed to high risk of pulmonary TB and TB mortality and treatment dropout, thus requiring special health surveillance interventions.

[The role of cultural identities and public health services in the municipalization process taken place in recent decades on small towns of Rio Grande do Sul, Brazil].

PubMed

Mejía, Margarita Gaviria; Périco, Eduardo; Oliveira, Laura Barbieri

2015-05-01

The paper presents a preliminary results of an ethnographic study in which we observe how is socially experienced the municipality process in six counties of the Forqueta Watershed in Rio Grande do Sul, where the municipal fragmentation has been used as an administrative strategy since the 1990s. Deal about cultural elements and social actions that support construction and/or reconstruction identities to define territories-county's borders. Sociological and anthropological theories have been used to think the identities and the assumption that the integration of social spaces into a territory creates the social necessity to produce a territorial identity, closely linked to a socio-political context and cultural setting. We realize that the decentralization process in small municipalities helps stem the rural exodus, being health services determinant in curbing the migratory flow that characterized these locations reality in recent decades as a result of the agribusiness growth. Today, in these same places, health services represent the main support of collective identity with the territory-county and, instead of emigration, stimulate the immigration.

[Working and health conditions of preschool teachers of the public school network of Pelotas, State of Rio Grande do Sul, Brazil].

PubMed

da Silva, Luciane Goulart; da Silva, Marcelo Cozzensa

2013-11-01

This study describes the working and health conditions of preschool teachers from the public school network in Pelotas, State of Rio Grande do Sul. A descriptive census was conducted in schools of the city and the state that offered preschool classes. The questionnaire included social and demographic, behavioral, nutritional, health and work issues. All teachers were female, more than 55% were classified as being overweight, 12.6% were smokers and 73% were not sufficiently physically active during their leisure time. With respect to the working conditions, 66.7% reported working in an uncomfortable posture, 40.5% considered the desks and furniture inadequate, 50.5% replied that the intervals between classes and activities are insufficient for resting. The prevalence of back, thoracic, neck and shoulder pain was high, and 17.8% tested positive for minor psychiatric disorders. The prevalence rates for occupational exposure and poor health conditions of preschool teachers are significant and can interfere in the quality of life and work of these individuals.

Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization.

PubMed

Svobodová, Kateřina; Semerád, Jaroslav; Petráčková, Denisa; Novotný, Čeněk

2018-05-30

Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, bla TEM , ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100 μg/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.

Workplace Accident Prevalence and Associated Factors among Tobacco Farm in São Lourenço do Sul-RS, Brazil.

PubMed

Zago, Adriana Marchon; Meucci, Rodrigo Dalke; Fiori, Nadia; Carret, Maria Laura Vidal; Faria, Neice Muller Xavier; Fassa, Anaclaudia Gastal

2018-05-01

Agriculture has the highest risk of accidents. In Brazil the reality of this situation is unknown owing to scarcity of studies and underreporting of workplace accidents in rural areas. This article aims to evaluate workplace accident prevalence and associated factors among tobacco farm in Sao Lourenco do Sul-RS, Brazil. Cross-sectional study with 488 tobacco farmers, assessing sociodemographic, behavioural, labour characteristics and association with workplace accidents occurring in their lifetime. The injury prevalence was 24%. Being male (PR 1.62; 95%CI 1.04-2.52), and tenant farmer (PR 1.87; 95%CI 1.29-2.72), bundling tobacco leaves (PR 2.00; 95%CI 1.14-3.52) and having minor psychiatric disorders (PR 1.58; 95%CI 1.06-2.35) were positively associated with accidents. 46% of serious injuries caused superficial lesions and 26% caused fractures. Rural workplace accident prevention policies need to be established, particularly for tobacco farming. Larger studies are needed to understand work process-related aspects that increase the risk of accidents.

Genetic identification of Rickettsia sp. strain Atlantic rainforest in an endemic area of a mild spotted fever in Rio Grande do Sul state, Southern Brazil.

PubMed

Figueiredo Voizzoni, Vinicius; Barbosa Silva, Arannadia; Medeiros Cardoso, Karen; Barbosa Dos Santos, Fernanda; Stenzel, Barbara; Amorim, Marinete; Vilges de Oliveira, Stefan; Salles Gazeta, Gilberto

2016-10-01

Rickettsia sp. strain Atlantic rainforest causes a less severe rickettsiosis, with two cases confirmed until now. The tick species Amblyomma ovale is appointed as the main vector of this bacterium. The southern region of Brazil has reported patients with spotted fever who have milder symptoms. In 2013, during an investigation of rickettsiosis cases, an A. ovale tick was found attached to a man in an area where there were two cases. The parasite was processed for molecular analysis and the rickettsial infection was confirmed based on phylogenetic analysis of genes ompA, ompB and geneD (sca4). In the present study the human pathogenic Rickettsia sp. strain Atlantic rainforest was identified in the state of Rio Grande do Sul, Southern Brazil. Since A. ovale, its main vector, is found frequently parasitizing dogs, animals that can cross international borders freely in southern Brazil, this bacteria can bring major concerns in terms of public health. Copyright © 2016 Elsevier B.V. All rights reserved.

Prevalence of minor psychiatric disorders in socio-educational agents in the state of Rio Grande do Sul.

PubMed

Greco, Patrícia Bitencourt Toscani; Magnago, Tânia Solange Bosi de Souza; Urbanetto, Janete de Souza; da Luz, Emanuelli Mancio Ferreira; Prochnow, Andrea

2015-01-01

To determine the prevalence and factors associated with minor psychiatric disorders (MPD) in socio-educational agents. It is a cross-sectional study with 381 socio-educational agents the Centers for Socio-Educational Services in the State of Rio Grande do Sul, Brazil. The Brazilian versions of the Scale of Demand-control-social support at work and the Self Reporting Questionnaire-20 have been applied. The results showed a prevalence of suspicion MPD of 50.1%. They showed to be related to suspicion of MPD : being female (55.7%), having age up to 44 years old (58.5%), no physical activity (57.4%), do not have time for leisure (75%), make use of medication (61.4%), require medical attention (56.9%) and psychological counseling (72.7%), not being satisfied with the workplace (61.7%) and need for time off from work (65.6%). The study provides important data about the mental health of agents, showing the need for the involvement of managers and of the health service of worker's health in planning actions to promote health of these workers.

[Prenatal care in public and private health services: a population-based survey in Rio Grande, Rio Grande do Sul State, Brazil].

PubMed

Cesar, Juraci A; Sutil, Andréa T; Santos, Gabriela B dos; Cunha, Carolina F; Mendoza-Sassi, Raúl A

2012-11-01

This study aimed to evaluate public and private prenatal care for women in Rio Grande, Rio Grande do Sul State, Brazil. Women who gave birth at the two local maternity hospitals from January 1 to December 31, 2010, answered a standardized questionnaire. The interview sites in the public sector were primary health care units with and without the Family Health Strategy (FHS) and outpatient clinics; the private sector included clinics operated by health plans and private physicians' offices. The chi-square test was used to compare proportions. The response rate was 97.2% (2,395 out of 2,464). Among the 23 target variables and indicators, seven showed a clear advantage for mothers who had received prenatal care under the FHS and six for health plan clinics and private offices. Four variables showed virtually universal coverage at all five study sites. Prenatal care showed better coverage for pregnant women treated in the private sector. Pregnant women treated under the FHS showed similar coverage to that in the private sector.

Anisotropy of Magnetic Susceptibility of Silicic Rocks From Quarries in the Vicinity of São Marcos, Rio Grande Do Sul, South Brazil: Implications for Emplacement Mechanisms.

NASA Astrophysics Data System (ADS)

Canon-Tapia, E.; Raposo, M. I. B.

2017-12-01

The Paraná-Etendeka Large Igneous Province includes felsic volcanic rocks whose mechanism of emplacement and location of their eruptive sources are controversial. Opening of several quarries of dimension stone near the city of Sao Marcos, Rio Grande do Sul, Brazil, offers a unique opportunity to study in detail some of those products. Here, we present the results of a study of the anisotropy of magnetic susceptibility (AMS) completed in some rocks that had been interpreted as the roots of volcanic conduits. Our results, and reexamination of the textural features of the rocks, lead to a reinterpretation that suggests that these rocks were emplaced subaerially, and involved assimilation and remelting of clastic components of previous lavas. The extremely high eruption temperatures of the lavas promoted the development of peperite-like textures and mixing patterns similar to those observed in plutonic environments. Due to the inferred conditions of emplacement, it is unlikely that the eruptive vents are located far from the area of study, therefore ruling out the long-travelled nature of these products.

Infection rate in adult patients with open fractures treated at the emergency hospital and at the ULBRA university hospital in Canoas, Rio Grande do Sul, Brazil.

PubMed

Guerra, Marcelo Teodoro Ezequiel; Gregio, Fernando Machado; Bernardi, Adriane; Castro, Cyntia Cordeiro de

2017-01-01

To identify the infection rate in adult patients with open fractures treated at two tertiary hospitals in the city of Canoas, Rio Grande do Sul, Brazil. This quantitative descriptive study was conducted at Hospital de Pronto Socorro de Canoas. Eligible participants were adults aged 18-60 years with open fractures who were admitted to the orthopedic trauma service from January to May 2014 and followed-up for one year. A total of 133 patients with open fractures were included; most were men (92.48%), with a mean age of 36 years. There was a predominance of Gustilo-Anderson type III fractures. The infection rate was 18.80%, being more frequent in Gustilo-Anderson type III fractures (72.00%). The most commonly observed bacteria were Staphylococcus aureus and Enterobacter aerogenes . The infection rate in open fractures of patients initially treated at the emergency department of HPSC was 18.8%. The infections occurred predominantly in Gustilo-Anderson type III fractures. The bacteria with the highest incidence in infections were Staphylococcus aureus and Enterobacter aerogenes .

Excessive consumption of dietary supplements among professionals working in gyms in Pelotas, Rio Grande do Sul State, Brazil, 2012.

PubMed

Cava, Tatiane Araujo; Madruga, Samanta Winck; Teixeira, Gesiane Dias Trindade; Reichert, Felipe Fossati; Silva, Marcelo Cozzensa da; Rombaldi, Airton José

2017-01-01

to investigate the prevalence and factors associated with excessive consumption of dietary supplements among professionals working at gyms in Pelotas, Rio Grande do Sul State, Brazil. this is a cross-sectional study with all local fitness professionals identified in 2012; excessive consumption of dietary supplements was defined as the use of three or more types of supplements simultaneously; multivariate analysis was carried out using Poisson regression with robust variance. 497 professionals were interviewed; the prevalence of excessive consumption of dietary supplements was 10.5% (95%CI 7.9;13.5); there was association with the male sex (PR=3.2; 95%CI 1.6;6.7) and with length of time of dietary supplement consumption ≥4 years when compared to <1 year (PR=2.8; 95%CI 1.7;4.7); lower consumption was found among professionals with higher levels of education, regardless of whether they had a degree in physical education or not (p=0,007). prevalence of excessive consumption of dietary supplements can be considered high and was associated with sociodemographic variables.

Luiz Rau creek and the city of Novo Hamburgo, Rio Grande do Sul.

PubMed

Schemes, C; Castilhos-Araujo, D; Lima-Magalhães, M

2015-12-01

This article presents a reflection on the past and current history, uses, and significance of the Luiz Rau creek to the municipality of Novo Hamburgo, Rio Grande do Sul. Its waters have always been important to the region, quenching the thirst of the local population and their livestock and providing venues for shared social interactions, but also as a destination for municipal industrial and household waste, which has polluted the waters of the creek. Our primary objective is to present and discuss these aspects with the purpose of elucidating the historical importance of this watercourse to the city of Novo Hamburgo. Toward that end, we conducted an exploratory survey to obtain the necessary inputs for such a discussion. We also employed texts from the now-defunct Jornal 5 de Abril and from Jornal NH, the highest-circulating newspaper in the region, to illustrate some situations experienced by the community. We found that municipal waste continues to be dumped into the creek, which has made it rather unloved by the local residents, but it remains firmly present in their daily lives.

Re-emergence of Chorioptes bovis (Acari: Psoroptidae) in cattle in the state of Rio Grande do Sul, Brazil.

PubMed

Vieira, Maria Isabel Botelho; Bordin, Tiago; Dall'Agnol, Bruno; Zanchin, Fabiane; Motta, Adriana Costa Da; Noro, Mirela

2014-01-01

Here we describe an outbreak of chorioptic mange in cattle, 56 years after its first identification in Brazil. Between the months of June and July 2011, dermatitis characterized by alopecia and crusted and thickened skin at the insertion of the tail and in the ischiorectal fossa was recognized in 40 (35.7%) out of 112 Holstein cows on a farm in the northeastern mesoregion of the state of Rio Grande do Sul, Brazil. After diagnosing mange caused by Chorioptes bovis, the cows were weighed and treated with 0.5% ivermectin, as a pour-on single dose, and were separated into two groups: cows in early lactation and those in late lactation. The survival rate of C. bovis and the healing rate in the two groups of infested cows were monitored every seven days through skin scrapings. After 28 days of evaluation, the cure rate through treatment was greater among cows in early lactation (p <0.0001). The survival rate of C. bovis was higher in cows in late lactation.

Psychiatric hospitalizations in the Rio Grande do Sul State (Brazil) from 2000 to 2011.

PubMed

Horta, Rogério Lessa; da Costa, Juvenal Soares Dias; Balbinot, Alexandre Didó; Watte, Guilherme; Teixeira, Vanesa Andina; Poletto, Simone

2015-01-01

To examine the variation in the rates of psychiatric hospitalization and the mean hospital stay time in the public health system in the state of Rio Grande do Sul, in the south of Brazil, from 2000 to 2011. This was an ecological study. Data were collected from DATASUS. The rates were obtained from diagnosis of admissions due to psychoactive substance use and to other causes, stratified by the gender of the patients. The data were analyzed using Poisson regression and Spearman correlation coefficient. Increasing hospitalization rates were observed for women with disorders due to substance use (p < 0.001) and other causes (p < 0.001), and among men with disorders due to the use of alcohol or other drugs (p < 0.001). This elevation of the rates remained statistically significant and inversely correlated to the length of hospital stay (p < 0.001). In a period of expansion of the local care networks for mental health, an increase in the occupancy of psychiatric beds in the state was noticed, with shorter length of stay and greater diversity of gender and causes of hospitalization.

[Healthcare profile of rabies pre-exposure prophylaxis in the state of Rio Grande do Sul, Brazil, 2007-2014].

PubMed

Mota, Roberta Silva Silveira da; Schuch, Luiz Filipe Damé; Schuch, Dóris Gómez Marcos; Osmari, Christieli Prestes; Guimarães, Tássia Gomes

2016-01-01

to describe the profile of healthcare provision regarding rabies pre-exposure prophylaxis (RPrEP) in the state of Rio Grande do Sul, Brazil, as compared with the Technical Standards for Rabies Prophylaxis in Humans. this was a descriptive study using Notifiable Disease Information System data on anti-rabies healthcare provided between 2007 and 2014. only 2.4% of anti-rabies healthcare related to RPrEP (5,721/239,245), 42.5% of these were veterinary, biology and zootechnics students and 10.3% were professionals from the same areas; individuals aged 20 to 64 accounted for 71.8% of the total number of people vaccinated and the frequency of this form of prophylaxis was 53.5/100,000 inhabitants for the state as a whole, varying between 13.1 to 185.1/100,000 inhabitants in the state's different health districts. RPrEP frequency was found to be low, suggesting that this rabies prevention tool has been neglected, leaving a large number of people at occupational risk.

Relation of the activities of the IPDF/INPE project (reforestation subproject) during the year 1979. [Mato Grosso do Sul, Brazil

NASA Technical Reports Server (NTRS)

Dejesusparada, N. (Principal Investigator); Filho, P. H.; Shimabukuro, Y. E.; Demedeiros, J. S.; Desantana, C. C.; Alves, E. C. M.

1981-01-01

The state of Mato Grosso do Sul was selected as the study area to define the recognizable classes of Eucalyptus spp. and Pinus spp. by visual and automatic analyses. For visual analysis, a preliminary interpretation key and a legend of 6 groups were derived. Based on these six groups, three final classes were defined for analysis: (1) area prepared for reforestation; (2) area reforested with Eucalyptus spp.; and (3) area reforested with Pinus spp. For automatic interpretation the area along the highway from Ribas do Rio Pardo to Agua Clara was classified into the following classes: eucalytus, bare soil, plowed soil, pine and "cerrado". The results of visual analysis show that 67% of the reforested farms have relative differences in area estimate below 5%, 22%, between 5% and 10%; and 11% between 10% and 20%. The reforested eucalyptus area is 17 times greater than the area of reforested pine. Automatic classification of eucalyptus ranged from 73.03% to 92.30% in the training areas.

Anisotropy of magnetic susceptibility of silicic rocks from quarries in the vicinity of São Marcos, Rio Grande do Sul, South Brazil: Implications for emplacement mechanisms

NASA Astrophysics Data System (ADS)

Cañón-Tapia, Edgardo; Raposo, M. Irene B.

2018-04-01

The Paraná-Etendeka Large Igneous Province includes acid volcanic rocks that can be found throughout its extension. Several aspects concerning those rocks remain controversial, including their mechanism of emplacement and location of their eruptive sources. Opening of several quarries of dimension stone near the city of Sao Marcos, Rio Grande do Sul, Brazil, offers a unique opportunity to study in detail the acid products. Here, we present the results of a study of the anisotropy of magnetic susceptibility (AMS) completed in some rocks that had been interpreted as the roots of volcanic conduits. Our results, and reexamination of the textural features of the rocks, lead to a reinterpretation that suggests that these rocks were emplaced subaerially, and involved assimilation and remelting of clastic components of previous products. Due to the inferred conditions of emplacement, it is unlikely that the eruptive vents are located far from the area of study, therefore ruling out the long-travelled nature of these products.

Taxonomic revision of the Neotropical genus Pityocera Giglio-Tos, 1896 (Diptera: Tabanidae: Scionini).

PubMed

Krolow, Tiago Kütter; Henriques, Augusto Loureiro; Gorayeb, Inocêncio De Sousa; Limeira-de-Oliveira, Francisco; Buestán, Jaime

2015-01-07

The genus Pityocera Giglio-Tos is revised based on examination of external morphology and genitalia of type material and specimens from Brazilian and foreign institutions. Five currently valid species in three subgenera are recognized: P. (Elaphella) cervus (Wiedemann, 1828); P. (Pityocera) festai Giglio-Tos, 1896; P. (Pseudelaphella) nana (Walker, 1850); P. (Pseudelaphella) nigribasis Fairchild, 1964; P. (Pseudelaphella) patellicornis (Kröber, 1930). Five new species are described: P. (Pseudelaphella) barrosi Gorayeb & Krolow sp. nov. (Brazil: Mato Grosso do Sul); P. (Pseudelaphella) gorayebi Limeira-de-Oliveira & Krolow sp. nov. (Brazil: Maranhão, Tocantins and Bahia); P. (Pseudelaphella) pernaquila Gorayeb & Krolow sp. nov. (Brazil: Pará and Rondônia); P. (Pseudelaphella) rhinolissa Krolow & Henriques sp. nov. (Brazil: Pará, Maranhão, Tocantins, Rondônia, Mato Grosso, Goiás and Mato Grosso do Sul; Bolivia: Santa Cruz); P. (Pseudelaphella) ecuadorensis Buestán & Krolow sp. nov. (Ecuador: Manabí, Guayas, Santa Elena and Loja). We provide diagnosis, descriptions, redescriptions, distribution records, illustrations and discussion for all species, as well as a key for identification of species. 

Analysis of the characteristics appearing in LANDSAT multispectral images in the geological structural mapping of the midwestern portion of the Rio Grande do Sul shield. M.S. Thesis - 25 Mar. 1982; [Brazil

NASA Technical Reports Server (NTRS)

Parada, N. D. J. (Principal Investigator); Ohara, T.

1982-01-01

The central-western part of Rio Grande do Sul Shield was geologically mapped to test the use of MSS-LANDSAT data in the study of mineralized regions. Visual interpretation of the images a the scale of 1:500,000 consisted, in the identification and analysis of the different tonal and textural patterns in each spectral band. After the structural geologic mapping of the area, using visual interpretation techniques, the statistical data obtained were evaluated, specially data concerning size and direction of fractures. The IMAGE-100 system was used to enlarge and enhance certain imagery. The LANDSAT MSS data offer several advantages over conventional white and black aerial photographs for geological studies. Its multispectral characteristic (band 6 and false color composition of bands 4, 5 and 7 were best suitable for the study). Coverage of a large imaging area of about 35,000 sq km, giving a synoptical view, is very useful for perceiving the regional geological setting.

Poaceae Pollen from Southern Brazil: Distinguishing Grasslands (Campos) from Forests by Analyzing a Diverse Range of Poaceae Species

PubMed Central

Radaeski, Jefferson N.; Bauermann, Soraia G.; Pereira, Antonio B.

2016-01-01

This aim of this study was to distinguish grasslands from forests in southern Brazil by analyzing Poaceae pollen grains. Through light microscopy analysis, we measured the size of the pollen grain, pore, and annulus from 68 species of Rio Grande do Sul. Measurements were recorded of 10 forest species and 58 grassland species, representing all tribes of the Poaceae in Rio Grande do Sul. We measured the polar, equatorial, pore, and annulus diameter. Results of statistical tests showed that arboreous forest species have larger pollen grain sizes than grassland and herbaceous forest species, and in particular there are strongly significant differences between arboreous and grassland species. Discriminant analysis identified three distinct groups representing each vegetation type. Through the pollen measurements we established three pollen types: larger grains (>46 μm), from the Bambuseae pollen type, medium-sized grains (46–22 μm), from herbaceous pollen type, and small grains (<22 μm), from grassland pollen type. The results of our compiled Poaceae pollen dataset may be applied to the fossil pollen of Quaternary sediments. PMID:27999585

[Evaluation of the inclusion of organic food from family-based agriculture in school food in municipalities of rural territories of the state of Rio Grande do Sul, Brazil].

PubMed

dos Santos, Fernanda; Fernandes, Patrícia Fogaça; Rockett, Fernanda Camboim; de Oliveira, Ana Beatriz Almeida

2014-05-01

Organic food enables the promotion of Food and Nutritional Safety (FNS) and sustainable regional development. In this context, the National School Food Program (NSFD) seeks to comply with the requirements of FNS. This study evaluated the inclusion of organic food in school food in the municipalities of rural territories of the state of Rio Grande do Sul by means of interviews with local managers. Eight territories were visited, albeit of its 153 municipalities only 102 comprised the sample for this study. Of these, 20.58% said they buy organic produce from family farms. The Center South Territory revealed the highest percentage of purchase, in which 40% of the municipalities visited purchased organic produce, followed by the Center Mountain Territory with 33.3%, while the lowest percentage was 7.1% in the Countryside Territory. The study identified the need for intersectoral action to develop organic production, as well as stimulate the consumption of these foods in the school environment, in order to meet the requirements of FNS.

Occurrence of two novel actinospore types (Cnidaria: Myxozoa) in fish farms in Mato Grosso do Sul state, Brazil.

PubMed

Milanin, Tiago; Atkinson, Stephen D; Silva, Márcia R M; Alves, Roberto G; Tavares, Luiz Eduardo R; Ribeiro, Amanda M; Maia, Antonio A M

2018-06-01

We investigated the involvement of oligochaetes in the life cycles of fresh water myxozoan parasites in Brazil. In a fish farm in the State of Mato Grosso do Sul, we examined 192 oligochaetes and found that two (1%) released Aurantiactinomyxon type actinospores. We identified infected oligochaetes by morphology: both were Pristina synclites, from family Naididae. This is the first report of the involvement of this species in the life cycle of myxozoans. Small-subunit ribosomal DNA sequences of Aurantiactinomyxon type 1 (1882 nt) and Aurantiactinomyxon type 2 (1900 nt) did not match any previously sequenced myxozoan in the NCBI database, with the highest BLAST search similarities of 83% with Myxobolus batalhensis MF361090 and 93% with Henneguya maculosus KF296344, respectively, and the two aurantiactinomyxons were only 75% similar to each other (over ~ 1900 bases). Phylogenetic analyses showed that Aurantiactinomyxon type 1 had closest affinities with myxozoans from fish hosts in Order Characiformes, and Aurantiactinomyxon type 2 had affinities with myxozoans from fish of Order Siluriformes.

[Epidemiological trends for malaria in the cities of the upper Paraguay River basin, Mato Grosso do Sul, Brazil 1990-1996].

PubMed

Matsumoto, W K; Vicente, M G; Silva, M A; de Castro, L L

1998-01-01

Through the Regional Office of the Brazilian National Health Foundation in the State of Mato Grosso do Sul, we obtained numerical data on malaria for the upper Paraguay basin (UPB): 159 cases in 1990, 126 in 1991, 135 in 1992, 61 in 1993, 143 in 1994, 41 in 1995, and 20 in 1996, the majority of which were imported cases. There were no autochthonous cases in 1990, and since 1991 the rates of over 15% dropped to around 1.60%. Imported cases, corresponding to 0. 63% in 1990, increased in 1991 and 1992 to some 1.50%, and to 3.28% in 1993. Induced cases were recorded only in 1991 and 1992 (less than 1%). Most cases were between 16 and 45 years of age. There was a predominance of Plasmodium vivax in the thick blood smears. Although autochthonous cases of malaria are not the majority, the disease is still an important public health problem in the UPB in the presence of the Anopheles (N.) darlingi vector and human migration into the region.

[Court-ordered access to treatment of rare genetic diseases: Fabry Disease in the state of Rio Grande do Sul, Brazil].

PubMed

Sartori Junior, Dailor; Leivas, Paulo Gilberto Cogo; Souza, Mônica Vinhas de; Krug, Bárbara Corrêa; Balbinotto, Giacomo; Schwartz, Ida Vanessa Doederlein

2012-10-01

Court-ordered access to high-cost drugs for rare genetic diseases, such as Fabry Disease (alpha-galactosidase-A deficiency), is a growing phenomenon as yet lacking systematic study. An observational, cross-sectional and retrospective study was conducted to characterize the lawsuits related to access to treatment for Fabry Disease by Enzyme Replacement Therapy in the State of Rio Grande do Sul prior to 2007. The study identified 13 lawsuits and 17 plaintiffs, 11 requesting alfa and 6 betagalsidase. The State of RS, the Federal Government, and 5 municipalities figured as defendants, in the form of joinder of parties or otherwise. There were 13 requests for interlocutory relief of which 12 were granted, and 2 sentences were handed down, both favorable. "Risk of death" was alleged by doctors in 4 prescriptions and by lawyers in the 13 lawsuits. The data suggest the lack of discussions combining aspects of medical efficacy and safety, cost-effectiveness, economic impact, and legal and constitutional arguments, which requires a specific policy for rare genetic diseases to standardize access to treatment.

Orthopantomography and INAIL assessment of dental injury. Istituto Nazionale per l'Assicurazione contro gli Infortuni sul Lavoro.

PubMed

Iorio, M; Robetti, I; Malavenda, P

1993-12-01

The protection of workers' health is defended by the Italian Constitution, and sees its most extensive application in the work of the INAIL (Istituto Nazionale per l'Assicurazione contro gli Infortuni sul Lavoro-National Institute for Assurance against Work-related Accidents). In the T.U. (Testo Unico-Complete Text of Legislation) 1124/1965, dental injury is compensated with fixed percentages for permanent impairment, depending on whether the prosthesis applied is effectual or otherwise. The INAIL's primary task of assigning the necessary treatment and recovering the claimant's occupational aptitude requires that it meets in full the cost of his dental rehabilitation. In Turin in 1981 a systematic procedure was set up for the provision and monitoring of the dental treatment received by the injured person. Legislation relating to prostheses has further widened the scope of dental treatment. To deal correctly with the legal medicine aspects of dental rehabilitation, the authors illustrate a working procedure which is based on the reading of radiological evidence in order to determine the prior situation, extent of injury, results of the treatment and evaluation of residual permanent injury.

Biomarkers and bioindicators of the environmental condition using a fish species (Pimelodus maculatus Lacepède, 1803) in a tropical reservoir in Southeastern Brazil.

PubMed

Araújo, F G; Morado, C N; Parente, T T E; Paumgartten, F J R; Gomes, I D

2018-05-01

The Funil Reservoir receives a large amount of xenobiotics from the Paraíba do Sul River (PSR) from large number of industries and municipalities in the watershed. This study aimed to assess environmental quality along the longitudinal profile of the Paraíba do Sul River-Funil Reservoir system, by using biomarkers and bioindicators in a selected fish species. The raised hypothesis is that Funil Reservoir acts as a filter for the xenobiotics of the PSR waters, improving river water quality downstream the dam. Two biomarkers, the ethoxyresorufin-O-deethylase activity (EROD), measured as fluorimetricly in S9 hepatic fraction, and the micronuclei frequency (MN), observed in erythrocytes of the cytoplasm, and three bioindicators, the hepatosomatic index (HSI), gonadosomatic index (GSI) and condition factor (CF) were used in Pimelodus maculatus, a fish species widely distributed in the system. Four zones were searched through a longitudinal gradient: 1, river upstream from the reservoir; 2, upper reservoir; 3, lower reservoir; 4, river downstream of the reservoir. EROD activity and HSI and GSI had significant differences among the zones (P<0.05). The upper reservoir had the lowest EROD activity and HSI, whereas the river downstream of the reservoir had the highest EROD and lowest GSI. The river upstream from the reservoir showed the highest HSI and GSI. It is suggested that the lowest environmental condition occur at the river downstream of the reservoir, where it seems to occur more influence of xenobiotics, which could be associated with hydroelectric plant operation. The hypothesis that Funil reservoir acts as a filter decanting pollution from the Paraíba do Sul River waters was rejected. These results are novel information on this subject for a native fish species and could be useful for future comparisons with other environments.

Down-regulation of the detoxifying enzyme NAD(P)H:quinone oxidoreductase 1 by vanadium in Hepa 1c1c7 cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anwar-Mohamed, Anwar; El-Kadi, Ayman O.S.

2009-05-01

Recent data suggest that vanadium (V{sup 5+}) compounds exert protective effects against chemical-induced carcinogenesis, mainly through modifying various xenobiotic metabolizing enzymes. In fact, we have shown that V{sup 5+} down-regulates the expression of Cyp1a1 at the transcriptional level through an ATP-dependent mechanism. However, incongruously, there is increasing evidence that V{sup 5+} is found in higher amounts in cancer cells and tissues than in normal cells or tissues. Therefore, the current study aims to address the possible effect of this metal on the regulation of expression of an enzyme that helps maintain endogenous antioxidants used to protect tissues/cells from mutagens, carcinogens,more » and oxidative stress damage, NAD(P)H:quinone oxidoreductase 1 (Nqo1). In an attempt to examine these effects, Hepa 1c1c7 cells and its AhR-deficient version, c12, were treated with increasing concentrations of V{sup 5+} in the presence of two distinct Nqo1 inducers, the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and isothiocyanate sulforaphane (SUL). Our results showed that V{sup 5+} inhibits the TCDD- and SUL-mediated induction of Nqo1 at mRNA, protein, and catalytic activity levels. At transcriptional level, V{sup 5+} was able to decrease the TCDD- and SUL-induced nuclear accumulation of Nrf2 and the subsequent binding to antioxidant responsive element (ARE) without affecting Nrf2 protein levels. Looking at post-transcriptional level; we found that V{sup 5+} did not affect Nqo1 mRNA transcripts turn-over rates. However, at the post-translational level V{sup 5+} increased Nqo1 protein half-life. In conclusion, the present study demonstrates that V{sup 5+} down-regulates Nqo1 at the transcriptional level, possibly through inhibiting the ATP-dependent activation of Nrf2.« less

Removal of antibiotics and antibiotic resistance genes from domestic sewage by constructed wetlands: Effect of flow configuration and plant species.

PubMed

Chen, Jun; Ying, Guang-Guo; Wei, Xiao-Dong; Liu, You-Sheng; Liu, Shuang-Shuang; Hu, Li-Xin; He, Liang-Ying; Chen, Zhi-Feng; Chen, Fan-Rong; Yang, Yong-Qiang

2016-11-15

This study aims to investigate the removal of antibiotics and antibiotic resistance genes (ARGs) in raw domestic wastewater by various mesocosm-scale constructed wetlands (CWs) with different flow configurations or plant species including the constructed wetland with or without plant. Six mesocosm-scale CWs with three flow types (surface flow, horizontal subsurface flow and vertical subsurface flow) and two plant species (Thaliadealbata Fraser and Iris tectorum Maxim) were set up in the outdoor. 8 antibiotics including erythromycin-H2O (ETM-H2O), monensin (MON), clarithromycin (CTM), leucomycin (LCM), sulfamethoxazole (SMX), trimethoprim (TMP), sulfamethazine (SMZ) and sulfapyridine (SPD) and 12 genes including three sulfonamide resistance genes (sul1, sul2 and sul3), four tetracycline resistance genes (tetG, tetM, tetO and tetX), two macrolide resistance genes (ermB and ermC), two chloramphenicol resistance genes (cmlA and floR) and 16S rRNA (bacteria) were determined in different matrices (water, particle, substrate and plant phases) from the mesocosm-scale systems. The aqueous removal efficiencies of total antibiotics ranged from 75.8 to 98.6%, while those of total ARGs varied between 63.9 and 84.0% by the mesocosm-scale CWs. The presence of plants was beneficial to the removal of pollutants, and the subsurface flow CWs had higher pollutant removal than the surface flow CWs, especially for antibiotics. According to the mass balance analysis, the masses of all detected antibiotics during the operation period were 247,000, 4920-10,600, 0.05-0.41 and 3500-60,000μg in influent, substrate, plant and effluent of the mesocosm-scale CWs. In the CWs, biodegradation, substrate adsorption and plant uptake all played certain roles in reducing the loadings of nutrients, antibiotics and ARGs, but biodegradation was the most important process in the removal of these pollutants. Copyright © 2016 Elsevier B.V. All rights reserved.

Evaluation of the organic matter sources using the δ13C composition of individual n-alkanes in sediments from Brazilian estuarine systems by GC/C/IRMS

NASA Astrophysics Data System (ADS)

Maioli, Otávio Luiz Gusso; de Oliveira, Cristiane Rossi; Dal Sasso, Marco Aurélio; Madureira, Luiz Augusto dos Santos; Azevedo, Débora de Almeida; de Aquino Neto, Francisco Radler

2012-12-01

The δ13C composition of individual n-alkanes (from C16 to C34) was measured from surface sediments of five Brazilian estuarine systems affected by different organic matter sources, such as harbor area, industries, urban centers and sugar cane crops, in order to determine the origins of the organic matter. The aliphatic hydrocarbon fraction was analyzed by gas chromatography-combustion-isotope ratio mass spectrometry (GC/C/IRMS). The levels of n-alkanes in the studied areas ranged from 0.34 to 18.14 μg kg-1, being relatively low in comparison to high polluted environments. The Carbon Preference Index (CPI) calculated in the C23-C34 range indicates that n-alkanes are mainly inherited from cuticular waxes of higher plants. The δ13C composition of all n-alkanes detected in the sediment samples ranged from -39.6 to -18.3‰ showing different sources for the studied estuarine systems. Through Principal Component Analysis (PCA) it was possible to verify the petrogenic influence in the n-alkane sources, especially in the Paraíba do Sul sediment samples. Differences up to 15‰ of the δ13C values between n-alkanes of odd and even carbon number (C26 and C27) also indicated mixture of petrogenic and biogenic sources in Paraíba do Sul River. High (less negative) δ13C n-alkane values of odd carbon number were obtained from two sampling sites located close to an ethanol plant, indicating residues discharge of sugar cane (C4 plant). Influence of C3 plants that are the main components of dense ombrophile forest was observed in the Itajaí-Açu sediments by the decrease of δ13C (about 10‰ compared to the Paraíba do Sul River δ13C).

Effect of the selective pressure of sub-lethal level of heavy metals on the fate and distribution of ARGs in the catchment scale.

PubMed

Xu, Yan; Xu, Jian; Mao, Daqing; Luo, Yi

2017-01-01

Our previous study demonstrated that high levels of antibiotic resistance genes (ARGs) in the Haihe River were directly attributed to the excessive use of antibiotics in animal agriculture. The antibiotic residues of the Xiangjiang River determined in this study were much lower than those of the Haihe River, but the relative abundance of 16 detected ARGs (sul1, sul2 and sul3, qepA, qnrA, qnrB, qnrD and qnrS, tetA, tetB, tetW, tetM, tetQ and tetO, ermB and ermC), were as high as the Haihe River particularly in the downstream of the Xiangjiang River which is close to the extensive metal mining. The ARGs discharged from the pharmaceutical wastewater treatment plant (PWWTP) are a major source of ARGs in the upstream of the Xiangjiang River. In the downstream, selective stress of heavy metals rather than source release had a significant influence on the distinct distribution pattern of ARGs. Some heavy metals showed a positive correlation with certain ARG subtypes. Additionally, there is a positive correlation between individual ARG subtypes and heavy metal resistance genes, suggesting that heavy metals may co select the ARGs on the same plasmid of antibiotic resistant bacteria. The co-selection mechanism between specific metal and antibiotic resistance was further confirmed by these isolations encoding the resistance genotypes to antibiotics and metals. To our knowledge, this is the first study on the fate and distribution of ARGs under the selective pressure exerted by heavy metals in the catchment scale. These results are beneficial to understand the fate, and to discern the contributors of ARGs from either the source release or the selective pressure by sub-lethal levels of environmental stressors during their transport on a river catchment scale. Copyright © 2016 Elsevier Ltd. All rights reserved.

Analysis of antimicrobial resistance genes detected in multidrug-resistant Salmonella enterica serovar Typhimurium isolated from food animals.

PubMed

Glenn, LaShanda M; Lindsey, Rebecca L; Frank, Joseph F; Meinersmann, Richard J; Englen, Mark D; Fedorka-Cray, Paula J; Frye, Jonathan G

2011-09-01

Multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium is the most prevalent penta-resistant serovar isolated from animals by the U.S. National Antimicrobial Resistance Monitoring System. Penta-resistant isolates are often resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline. To investigate MDR in Salmonella Typhimurium (including variant 5-), one isolate each from cattle, poultry, and swine with at least the ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline phenotype were selected for each year from 1997 to 2007 (n = 33) for microarray analysis of antimicrobial resistance, incompatibility IncA/C, and HI1 plasmid genes. Cluster analysis based on these data separated 31 of the isolates into two groups A and B (15 and 16 isolates, respectively). Isolates in group A were phage type DT104 or U302 and were mostly swine isolates (7/15). Genes detected included intI1, bla(PSE-1), floR, aadA, sulI, tet(G), and tetR, which are often found in Salmonella Genomic Island I. Isolates in group B had numerous IncA/C plasmid genes detected and were mostly cattle isolates (9/16). Genes detected included bla(CMY-2), floR, aac(3), aadA, aphA1, strA, strB, sulI, sulII, dfrA, dhf, tet(A)(B)(C)(D), and tetR, which are often found on MDR-AmpC IncA/C plasmids. The IncA/C replicon was also detected in all group B isolates. The two remaining isolates did not cluster with any others and both had many HI1 plasmid genes detected. Linkage disequilibrium analysis detected significant associations between plasmid replicon type, phage type, and animal source. These data suggest that MDR in Salmonella Typhimurium is associated with DT104/Salmonella Genomic Island I or IncA/C MDR-AmpC encoding plasmids and these genetic elements have persisted throughout the study period.

Tracking antibiotic resistance genes in soil irrigated with dairy wastewater.

PubMed

Dungan, Robert S; McKinney, Chad W; Leytem, April B

2018-09-01

The application of dairy wastewater to agricultural soils is a widely used practice to irrigate crops and recycle nutrients. In this study, small-scale field plots were irrigated monthly (6 times) with dairy wastewater (100%), wastewater diluted to 50% with irrigation (canal) water, and diluted wastewater spiked with copper sulfate (50 mg Cu L -1 ), while control plots were irrigated with canal water. In addition, half of all plots were either planted with wheat or were left as bare soil. Biweekly soil samples were collected during this period and processed to determine the occurrence and abundance of antibiotic resistance genes [bla CTX-M-1 , erm(B), sul1, tet(B), tet(M), and tet(X)] and a class 1 integron-integrase gene (intI1) via quantitative real-time PCR (qPCR). Only sul1 and tet(X) were detected in soil (3 out of 32 samples) before the wastewater treatments were applied. However, the occurrence and relative abundance (normalized to 16S rRNA gene copies) of most genes [erm(B), intI1, sul1, and tet(M)] increased dramatically after wastewater irrigation and levels were maintained during the entire study period. bla CTX-M-1 was the only gene not detected in wastewater-treated soils, which is likely related to its absence in the dairy wastewater. Relative gene levels in soil were found to be statistically similar among the treatments in most cases, regardless of the wastewater percentage applied and presence or absence of plants. The key result from this study is that dairy wastewater irrigation significantly enlarges the reservoir of ARGs and intI1 in soils, while detection of these genes rarely occurred in soil irrigated only with canal water. In addition, elevated levels of Cu in the wastewater and treated soil did not produce a concomitant increase of the ARG levels. Published by Elsevier B.V.

Post-collisional subvolcanic rhyolites associated with the Neoproterozoic Pelotas Batholith, southern Brazil

NASA Astrophysics Data System (ADS)

Oliveira, Diego Skieresz de; Sommer, Carlos Augusto; Philipp, Ruy Paulo; Lima, Evandro Fernandes de; Basei, Miguel Ângelo Stipp

2015-11-01

Neoproterozoic volcanic and subvolcanic rhyolitic systems in southernmost Brazil are correlated with acid magmatism linked to different petrotectonic associations of the Sul-Rio-Grandense Shield. A portion of this volcanism in the Dom Feliciano Belt is associated with the Pelotas Batholith, which resulted from magmatic episodes associated with the Ediacaran post-collisional evolution of southern Brazil. Ana Dias Rhyolite is the main subvolcanic occurrence of this volcanism that took place in the Quitéria region, in the central part of Rio Grande do Sul State. The acid magmatism has been commonly associated with the most differentiated granite suite phases during the final stages of emplacement of the Pelotas Batholith. The Ana Dias Rhyolite is characterized as an intrusive body with rocks that present a porphyritic to seriated texture and a gradational variation to fine-grained equigranular rocks. New zircon U-Pb dating indicates crystallization age of 581.9 ± 1.9 Ma for the Ana Dias Rhyolite. Geochemistry data characterize the rhyolites as belonging to the alkaline series; they present a metaluminous to peraluminous character; elevated SiO2 and alkali concentrations, high FeOt/FeOt + MgO ratios and agpaitic index; and low Al2O3, CaO, and MgO contents. The Zr, Rb, Y, Nb, and Ga concentrations are moderate when compared with the relatively low Ba and Sr contents. These geochemistry characteristics are common in acid magmas with alkaline affinity. The behavior of certain trace elements and REE demonstrate enrichment in more incompatible elements, in addition to the negative anomaly of Ba, the slight enrichment in Ce relative to adjacent elements, as well as the enrichment in K2O and Rb relative to Nb, suggesting magmas derived from mantle sources enriched in incompatible elements with some crustal contamination. The chemical characteristics are similar to those of A-type granites associated with Neoproterozoic post-collision magmatism in the Sul-Rio-Grandense Shield.

Plants used as antidiabetics in popular medicine in Rio Grande do Sul, southern Brazil.

PubMed

Trojan-Rodrigues, M; Alves, T L S; Soares, G L G; Ritter, M R

2012-01-06

Plants are widely as antidiabetics. The study of these plants is essential because many of them may have undesirable effects, such as acute or chronic toxicity; or their use may even delay or discourage the adoption of the proper and effective treatment. The present study surveyed the plant species that are popularly used to treat diabetes mellitus in the state of Rio Grande do Sul in southern Brazil. Sixteen ethnobotanical surveys performed in the state were consulted, and the species used to treat diabetes were listed. For species cited in at least two of the studies, scientific data related to antidiabetic activity were searched in the ISI Knowledge database. The scientific binomial of each species was used as keywords, and data found in review papers were also included. A total of 81 species in 42 families were mentioned; the most important families were Asteraceae and Myrtaceae. Twenty eight species were cited at least twice as being used to treat diabetes in the state. For 11 of these, no scientific data regarding antidiabetic activity could be located. The species most frequently mentioned for use with diabetes were Syzygium cumini (Myrtaceae) and Bauhinia forficata (Fabaceae), in 12 studies each, followed by Sphagneticola trilobata (Asteraceae), in six studies; and Baccharis trimera (Asteraceae), Bidens pilosa (Asteraceae), Cynara scolymus (Asteraceae), and Leandra australis (Melastomataceae) in four studies each. Bauhinia forficata and Syzygium cumini have been studied in more detail for antidiabetic activity. A considerable number of plant species are traditionally used for the treatment of diabetes melitus in the Rio Grande do Sul State. The majority of those plants that have been studied for antidiabetic activity showed promising results, mainly for Bauhinia forficata and Syzygium cumini. However, for most of the plants mentioned, the studies are not sufficient to guarantee the efficacy and safety in the use of these plants in the treatment against diabetes. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Removal of antibiotics and antibiotic resistance genes from domestic sewage by constructed wetlands: Optimization of wetland substrates and hydraulic loading.

PubMed

Chen, Jun; Wei, Xiao-Dong; Liu, You-Sheng; Ying, Guang-Guo; Liu, Shuang-Shuang; He, Liang-Ying; Su, Hao-Chang; Hu, Li-Xin; Chen, Fan-Rong; Yang, Yong-Qiang

2016-09-15

This study aimed to assess removal potential of antibiotics and antibiotic resistance genes (ARGs) in raw domestic wastewater by various mesocosm-scale horizontal subsurface-flow constructed wetlands (CWs) planted Cyperus alternifolius L. with different design parameters. Twelve CWs with three hydraulic loading rates (HLR 10, 20 and 30cm/day) and four substrates (oyster shell, zeolite, medical stone and ceramic) were set up in order to select the best optimized wetland. The result showed that 7 target antibiotics compounds including erythromycin-H2O, lincomycin, monensin, ofloxacin, sulfamerazine, sulfamethazine and novobiocin were detected, and all selected 18 genes (three sulfonamide resistance genes (sul1, sul2 and sul3), four tetracycline resistance genes (tetG, tetM, tetO and tetX), two macrolide resistance genes (ermB and ermC), three quinolone resistance genes (qnrB, qnrD and qnrS) and four chloramphenicol resistance genes (cmlA, fexA, fexB and floR)) and two integrase genes (int1 and int2) were positively detected in the domestic wastewaters. The aqueous removal rates of the total antibiotics ranged from17.9 to 98.5%, while those for the total ARGs varied between 50.0 and 85.8% by the mesocosm-scale CWs. After considering their aqueous removal rates in combination with their mass removals, the CW with zeolite as the substrate and HLR of 20cm/day was selected as the best choice. Combined chemical and biological analyses indicate that both microbial degradation and physical sorption processes were responsible for the fate of antibiotics and ARGs in the wetlands. The findings from this study suggest constructed wetlands could be a promising technology for the removal of emerging contaminants such as antibiotics and ARGs in domestic wastewater. Copyright © 2015 Elsevier B.V. All rights reserved.

Stability-Indicating HPTLC Method for Studying Stress Degradation Behavior of Sulbutiamine HCl.

PubMed

Farid, Nehal F; Abdelwahab, Nada S

2016-04-01

Sulbutiamine (SUL) is an ester of thiazides with neurotropic action. A new stability indicating HPTLC method has been developed and validated for the determination of SUL in the presence of different degradation products. The drug was subjected to different stress conditions following ICH strategy such as hydrolytic degradation (neutral, alkaline and acidic hydrolysis), oxidation, photodegradation and dry heat degradation. The drug demonstrated degradation under all decomposition conditions except neutral hydrolysis and dry heat, where the drug was completely degraded with 0.1 N NaOH, 1 N HCl and 30% H2O2 while it was partially degradaed by 0.1 N HCl, 3% H2O2 and UV light. Structure elucidation of the resulting degradation products was performed using ESI-Q-MS-MS. A well-defined peak for SUL was obtained at Rf = 0.46 and was completely separated from all obtained degradation products. Chromatographic separation was carried out on HPTLC aluminum plates precoated with silica gel 60 F254 using acetone-methylene chloride-ammonia buffer (pH 8.5 ± 0.2) (7:3:0.5, v/v) as a developing system. Densitometric scanning of the separated peaks was performed at 254 nm. System suitability testing parameters were calculated to ascertain the quality performance of the developed method. The method was validated with respect to USP guidelines regarding accuracy, precision, specificity, robustness and ruggedness. Good correlation coefficients were achieved in the range of 0.4-5.0 µg/band, and the limit of detection and limit of quantitation were found to be 0.11 and 0.33 µg/band, respectively. The utility of the suggested method was verified by application to Arcalion forte® tablets where no interference from additives was found. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Phenotypic and genotypic bacterial antimicrobial resistance in liquid pig manure is variously associated with contents of tetracyclines and sulfonamides.

PubMed

Hölzel, C S; Harms, K S; Küchenhoff, H; Kunz, A; Müller, C; Meyer, K; Schwaiger, K; Bauer, J

2010-05-01

Antibiotic residues as well as antibiotic-resistant bacteria in environmental samples might pose a risk to human health. This study aimed to investigate the association between antibiotic residues and bacterial antimicrobial resistance in liquid pig manure used as fertilizer. Concentrations of tetracyclines (TETs) and sulfonamides (SULs) were determined by liquid chromatography-mass spectrometry in 305 pig manure samples; antibiotic contents were correlated to the phenotypic resistance of Escherichia coli (n = 613) and enterococci (n = 564) towards up to 24 antibiotics. In 121 samples, the concentration of the TET resistance genes tet(M), tet(O) and tet(B) was quantified by real-time-PCR. TETs were found in 54% of the samples. The median sum concentration of all investigated TETs in the positive samples was 0.73 mg kg(-1). SULs were found with a similar frequency (51%) and a median sum concentration of 0.15 mg kg(-1) in the positive samples. Associated with the detection of TETs and/or SULs, resistance rates were significantly elevated for several substances - some of them not used in farm animals, e.g. chloramphenicol and synercid. In addition, multiresistant isolates were found more often in samples containing antibiotics. Analysis of the resistance genes tet(M) and tet(O) already showed a significant increase in their concentrations - but not in tet(B) - in the lowest range of total TET concentration. Mean tet(M) concentrations increased by the factor of 4.5 in the TET concentration range of 0.1-1 mg kg(-1), compared to negative manure samples. Antibiotic contamination of manure seems to be associated with a variety of changes in bacterial resistance, calling for a prudent use of antibiotics in farm animals. This study provides an interdisciplinary approach to assess antimicrobial resistance by combining the microbiological analysis of bacterial resistance with high quality chemical analysis of antibiotic residues in a representative number of environmental samples.

[Drug resistance profile of Mycobacterium tuberculosis in the state of Mato Grosso do Sul, Brazil, 2000-2006].

PubMed

Marques, Marli; Cunha, Eunice Atsuko Totumi; Ruffino-Netto, Antonio; Andrade, Sonia Maria de Oliveira

2010-01-01

To determine the drug resistance profile of Mycobacterium tuberculosis in the state of Mato Grosso do Sul, Brazil, between 2000 and 2006. Descriptive study of reported tuberculosis cases in the Brazilian Case Registry Database. We included only those cases in which M. tuberculosis culture was positive and sensitivity to drugs (rifampicin, isoniazid, streptomycin and ethambutol) was tested. Löwenstein-Jensen and Ogawa-Kudoh solid media were used for cultures, as was an automated liquid medium system. Sensitivity tests were based on the proportion method. Among the 783 cases evaluated, males predominated (69.7%), as did patients in the 20-49 year age bracket (70%), a diagnosis of pulmonary tuberculosis (94.4%) and positive HIV serology (8.6%); 645 (82.4%) were new cases, and 138 (17.6%) had previously been treated. Resistance to at least one drug was found in 143 cases (18.3%). The primary resistance (PR) rate was, respectively, 8.1%, 1.6%, 2.8% and 12.4%, for monoresistance, multidrug resistance (MDR), other patterns of resistance and resistance to at least one drug, whereas the acquired resistance (AR) rate was 14.5%, 20.3%, 10.9% and 45.7%, respectively, and the combined resistance (CR) rate was 9.2%, 4.9%, 4.2% and 18.3%, respectively. In PR, streptomycin was the most common drug, whereas isoniazid was the most common in AR and CR (7.2% and 3.7%, respectively). These high levels of resistance undermine the efforts for tuberculosis control in Mato Grosso do Sul. Acquired MDR was 12.7 times more common than was primary MDR, demonstrating that the previous use of drug therapy is an indicator of resistance. These levels reflect the poor quality of the health care provided to these patients, showing the importance of using the directly observed treatment, short course strategy, as well as the need to perform cultures and sensitivity tests for the early diagnosis of drug resistance.

Knowledge-In-Action: An Example with Rigid Body Motion

ERIC Educational Resources Information Center

Da Costa, Sayonara Salvador Cabral; Moreira, Marco Antonio

2005-01-01

This paper reports the analysis of the resolution of a paper-and-pencil problem, by eight undergraduate students majoring in engineering (six) and physics (two) at the Pontifcia Universidade Catlica do Rio Grande do Sul, in Porto Alegre, Brazil. The problem concerns kinetics of a rigid body, and the analysis was done in the light of Johnson-Lairds…

The Better Early Childhood Development Program: An Innovative Brazilian Public Policy

ERIC Educational Resources Information Center

Schneider, Alessandra; Ramires, Vera Regina; Paiva, Maria da Graca Gomes; Almeida, Leila

2009-01-01

This paper presents the pioneering experience of the Programa Primeira Infancia Melhor (the Better Early Childhood Development Program), also known as "PIM" that has been developed since 2003 in the State of Rio Grande do Sul, Brazil. PIM's goal is to "provide guidance to families, based on their own culture and experiences, to…

Final Report for Low Pressure Tests of the CPU-400 Pilot ...

EPA Pesticide Factsheets

... t - >r ;y|ij;ist r,d^ (l . i 1 ' 'ji T rrtx i :lf: :" ' t*1" ' ' • n ' f] ; 0 f'1 !t 'i . 3r ~'':Vn to !,1 .UPS So : ' W-istc Fuel ?j ...

Description of the female, pupa and gall of Pisphondylia brasiliensis Couri and Maia, 1992 (Diptera: Cecidomyiidae, Schizomyiina) with new records.

PubMed

Maia, V C; Fleury, G; Soares, G L G; Isaias, R M S

2010-11-01

The gall of Pisphondylia brasiliensis on Guapira opposita, its female and pupa are described and illustrated. The geographic distribution of this species is now widened to Minas Gerais and Rio Grande do Sul (Brazil). For the first time, a female of the genus is described.

The Virtual Learning Environment ROODA: An Institutional Project of Long Distance Education

ERIC Educational Resources Information Center

Behar, Patricia Alejandra; Leite, Silvia Meirelles

2006-01-01

This article describes ROODA (http://www.homer.nuted.edu.ufrgs.br), a virtual learning environment and one of the official Long Distance Education platforms that has been in use since 2005 at the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. It is free software that integrates syncronous and assyncronous…

The Process of Social In(ex)clusion in Schools: Data from Reality.

ERIC Educational Resources Information Center

de Castro, Marta Luz Sisson; Menezes, Janaina S. S.

Brazil has one of the most inequitable income distributions in the world, and schools perpetuate the processes of social exclusion. This paper examines processes of social exclusion in the municipal elementary schools of the state of Rio Grande do Sul, Brazil. Interviews with municipal superintendents focused on the schools' welfare services,…

Water balance in paired watersheds with eucalyptus and degraded grassland in Pampa biome

USDA-ARS?s Scientific Manuscript database

Rangelands of the Pampa biome, which cover regions of Argentina, Uruguay and Brazil (176,496 km2 – 2.07% of Brazilian territory and 63% of Rio Grande do Sul State territory, southern region of Brazil) in South America (total area of 750,000 km2), are being substituted by crops and commercial eucalyp...

The Pedagogical and Ethical Legacy of a "Successful" Educational Reform: The "Citizen School Project"

ERIC Educational Resources Information Center

Fischman, Gustavo E.; Gandin, Luis Armando

2016-01-01

The "Citizen School Project" ("Escola Cidadã") was implemented from 1993 to 2004 in Porto Alegre, capital of the Brazilian state of Rio Grande do Sul. This article presents the conception behind the "Citizen School Project," the basic mechanisms created to implement and evaluate its strengths and weaknesses, and some…

AFOSR Technical Summaries.

DTIC Science & Technology

1988-03-01

0 0 C C4’C ~~~~4𔃾’SENk4󈧰 L +0 InAILS EC ’*U UESCW C 4𔃺 .CU SUL >0LE - II a- LOVW - M0r >ff4’ LU).- C%5C0- -L--fllA- 104 -100 4.1) 41S0 I 0-O A 0 4

US EPA, Pesticide Product Label, MAQUAT 128-NHQ, 03/26/2013

EPA Pesticide Factsheets

2013-04-06

... '"-'•v •M 1 i «z! «©•• if|i ifA ;m*iu1 yen m••^ t' .•*. &7-: ^ : } ... If ' u - §• ^ sW:S * i ™.i§i oS£sI-°J^ •"'K Su^l^ §£ °pS"^ rS" — — „- S ^sin'tzja"!"-? ...

Regional and field-specific factors affect the composition of Fusarium head blight pathogens in subtropical no-till wheat agroecosystem of Brazil

USDA-ARS?s Scientific Manuscript database

A multiyear survey of >200 wheat fields in Paraná (PR) and Rio Grande do Sul (RS) states was conducted to assess the extent and distribution of Fusarium graminearum species complex (FGSC) diversity in the southern Brazilian wheat agroecosystem. Five species and three trichothecene genotypes were fou...

"I Will Count My Sheep": Creativity and the "Everyday Life Project"

ERIC Educational Resources Information Center

Torres, Cecilia A.; Souza, Jusamara

2005-01-01

This article reports on a project called, "Everyday life as a perspective on music education in the classroom." Part of this project involved the construction of texts and lyrics by a group of students from year 5, in weekly music lessons at a regular primary school from Porto Alegre, Rio Grande do Sul State, Brazil. The article…

Wheat cultivation: Identifying and estimating area by means of LANDSAT data

NASA Technical Reports Server (NTRS)

Dejesusparada, N. (Principal Investigator); Mendonca, F. J.; Cottrell, D. A.; Tardin, A. T.; Lee, D. C. L.; Shimabukuro, Y. E.; Moreira, M. A.; Delima, A. M.; Maia, F. C. S.

1981-01-01

Automatic classification of LANDSAT data supported by aerial photography for identification and estimation of wheat growing areas was evaluated. Data covering three regions in the State of Rio Grande do Sul, Brazil were analyzed. The average correct classification of IMAGE-100 data was 51.02% and 63.30%, respectively, for the periods of July and of September/October, 1979.

History from Children's Perspectives: Learning to Read and Write Historical Accounts Using Family Sources

ERIC Educational Resources Information Center

Schmidt, Maria Auxiliadora; Garcia, Tania Maria F. Braga

2010-01-01

This paper presents the results of an investigation which was part of a project called "Recreating Histories". It is concerned with the analysis of historical narratives created by the children who participated in the project and an analysis of historical sources kept by families who live in Campina Grande do Sul (Brazil). It draws on…

Theatre and Dance with Deaf Students: Researching Performance Practices in a Brazilian School Context

ERIC Educational Resources Information Center

Berselli, Marcia; Lulkin, Sergio A.

2017-01-01

The article presents performance practices created with deaf students in the project "Theater and dance with deaf students," an outreach activity of the Federal University of Rio Grande do Sul (UFRGS). The project took place in the Bilingual Deaf Municipal Elementary School Salomão Watnick in Porto Alegre, Brazil from 2013 to 2015. The…

[Study of sand flies (Diptera, Psychodidae, Phlebotominae) in the urban area of Campo Grande, Mato Grosso do Sul State, Brazil, from 1999 to 2000].

PubMed

de Oliveira, Alessandra Gutierrez; Andrade Filho, José Dilermando; Falcão, Alda Lima; Brazil, Reginaldo Peçanha

2003-01-01

From February 1999 to February 2000, sand flies were captured weekly with CDC light traps at five sites in the urban area of Campo Grande, Mato Grosso do Sul State, Brazil. Traps were placed in 11 different ecotopes in the environment (ground level, tree canopies, and forest edge) and the peridomicile (chicken coops and banana trees). A total of 1,245 sand flies were captured, belonging to 28 species: 4 species from genus Brumptomyia Fran a & Parrot, 1921 and 24 from genus Lutzomyia Fran a, 1924. The species were: B. avellari, B. brumpti, B. galindoi, B. pintoi, L. aragaoi, L. bourrouli, L. campograndensis, L. cerradincola, L. christenseni, L. claustrei, L. cortelezzii, L. corumbaensis, L. cruzi, L. damascenoi, L. flaviscutellata, L. hermanlenti, L. lenti, L. longipalpis, L. longipennis, L. migonei, L. punctigeniculata, L. quinquefer, L. renei, L. shannoni, L. sordellii, L. teratodes, L. termitophila, and L. whitmani. L. longipalpis and L. cruzi, vectors of visceral leishmaniasis, and L. whitmani, L. flaviscutellata and L. migonei, vectors of cutaneous leishmaniasis, were captured in the urban area. The most frequent species were L. termitophila, L. aragaoi, L. lenti, L. longipennis, and L. longipalpis.

[Necessities of professionals of the family health teams from the "4a Coordenadoria Regional de Saúde do Estado do Rio Grande do Sul" for training and improvement].

PubMed

Ferreira, Maria Evanir Vicente; Schimith, Maria Denise; Cáceres, Nilton Carlos

2010-08-01

This study had the aim to verify the necessities of training and improvement of the 4th Regional Coordination in Health of Rio Grande do Sul State family health teams. The data collection was carried out through the application of questionnaires with objective questions, composed by two parts: field and competence nucleus. For data analyses, Normality, Chi-Square and G tests were used as statistical procedures for Easy and Difficult attributions indicated by the professionals. Professionals have shown less difficulty with generic attributions, related with basic methods and techniques of each health area. However, they have revealed a lot of difficulties with attributions relating to the search of new partners in health inside communities, stimulation of the people participation in the discussion of health rights, and completion of the SIA/SIAB forms, and others. Even after 12 years of the foundation of the Family Health Program (PSF), several difficulties are still observed concerning the way to work with this new health strategy in Brazil. Proposals are made for the complete understanding and improvement of the PSF strategy.

Simultaneous selection for cowpea (Vigna unguiculata L.) genotypes with adaptability and yield stability using mixed models.

PubMed

Torres, F E; Teodoro, P E; Rodrigues, E V; Santos, A; Corrêa, A M; Ceccon, G

2016-04-29

The aim of this study was to select erect cowpea (Vigna unguiculata L.) genotypes simultaneously for high adaptability, stability, and yield grain in Mato Grosso do Sul, Brazil using mixed models. We conducted six trials of different cowpea genotypes in 2005 and 2006 in Aquidauana, Chapadão do Sul, Dourados, and Primavera do Leste. The experimental design was randomized complete blocks with four replications and 20 genotypes. Genetic parameters were estimated by restricted maximum likelihood/best linear unbiased prediction, and selection was based on the harmonic mean of the relative performance of genetic values method using three strategies: selection based on the predicted breeding value, having considered the performance mean of the genotypes in all environments (no interaction effect); the performance in each environment (with an interaction effect); and the simultaneous selection for grain yield, stability, and adaptability. The MNC99542F-5 and MNC99-537F-4 genotypes could be grown in various environments, as they exhibited high grain yield, adaptability, and stability. The average heritability of the genotypes was moderate to high and the selective accuracy was 82%, indicating an excellent potential for selection.

Sand fly captures with Disney traps in area of occurrence of Leishmania (Leishmania) amazonensis in the state of Mato Grosso do Sul, mid-western Brazil.

PubMed

Dorval, Maria Elizabeth Cavalheiros; Alves, Tulia Peixoto; Cristaldo, Geucira; Rocha, Hilda Carlos da; Alves, Murilo Andrade; Oshiro, Elisa Teruya; Oliveira, Alessandra Gutierrez de; Brazil, Reginaldo Peçanha; Galati, Eunice Aparecida Bianchi; Cunha, Rivaldo Venancio da

2010-01-01

The work was conducted to study phlebotomine fauna (Diptera: Psychodidae) and aspects of American cutaneous leishmaniasis transmission in a forested area where Leishmania (Leishmania) amazonensis occurs, situated in the municipality of Bela Vista, State of Mato Grosso do Sul, Brazil. The captures were conducted with modified Disney traps, using hamster (Mesocricetus auratus) as bait, from May 2004 to January 2006. Ten species of phlebotomine sandflies were captured: Brumptomyia avellari, Brumptomyia brumpti, Bichromomyia flaviscutellata, Evandromyia bourrouli, Evandromyia lenti, Lutzomyia longipalpis, Psathyromyia campograndensis, Psathyromyia punctigeniculata, Psathyromyia shannoni and Sciopemyia sordellii. The two predominant species were Ev bourrouli (57.3%) and Bi flaviscutellata (41.4%), present at all sampling sites. Two of the 36 hamsters used as bait presented natural infection with Leishmania. The parasite was identified as Leishmania (Leishmania) amazonensis. Analysis of the results revealed the efficiency of Disney traps for capturing Bichromomyia flaviscutellata and the simultaneous presence of both vector and the Leishmania species transmitted by the same can be considered a predictive factor of the occurrence of leishmaniasis outbreaks for the human population that occupies the location.

A review of the influence of treatment strategies on antibiotic resistant bacteria and antibiotic resistance genes.

PubMed

Sharma, Virender K; Johnson, Natalie; Cizmas, Leslie; McDonald, Thomas J; Kim, Hyunook

2016-05-01

Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in the aquatic environment have become an emerging contaminant issue, which has implications for human and ecological health. This review begins with an introduction to the occurrence of ARB and ARG in different environmental systems such as natural environments and drinking water resources. For example, ARG or ARB with resistance to ciprofloxacin, sulfamethoxazole, trimethoprim, quinolone, vancomycin, or tetracycline (e.g., tet(A), tet(B), tet(C), tet(G), tet(O), tet(M), tet(W), sul I, and sul II) have been detected in the environment. The development of resistance may be intrinsic, may be acquired through spontaneous mutations (de novo), or may occur due to horizontal gene transfer from donor bacteria, phages, or free DNA to recipient bacteria. An overview is also provided of the current knowledge regarding inactivation of ARB and ARG, and the mechanism of the effects of different disinfection processes in water and wastewater (chlorination, UV irradiation, Fenton reaction, ozonation, and photocatalytic oxidation). The effects of constructed wetlands and nanotechnology on ARB and ARG are also summarized. Copyright © 2015 Elsevier Ltd. All rights reserved.

Chlamydophila psittaci in free-living Blue-fronted Amazon parrots (Amazona aestiva) and Hyacinth macaws (Anodorhynchus hyacinthinus) in the Pantanal of Mato Grosso do Sul, Brazil.

PubMed

de Freitas Raso, Tânia; Seixas, Gláucia Helena Fernandes; Guedes, Neiva Maria Robaldo; Pinto, Aramis Augusto

2006-10-31

Chlamydophila psittaci (C. psittaci) infection was evaluated in 77 free-living nestlings of Blue-fronted Amazon parrots (Amazona aestiva) and Hyacinth macaws (Anodorhynchus hyacinthinus) in the Pantanal of Mato Grosso do Sul, Brazil. Tracheal and cloacal swab samples from 32 wild parrot and 45 macaw nestlings were submitted to semi-nested PCR, while serum samples were submitted to complement fixation test (CFT). Although all 32 Amazon parrot serum samples were negative by CFT, cloacal swabs from two birds were positive for Chlamydophila DNA by semi-nested PCR (6.3%); these positive birds were 32 and 45 days old. In macaws, tracheal and cloacal swabs were positive in 8.9% and 26.7% of the samples, respectively. Complement-fixing antibodies were detected in 4.8% of the macaw nestlings; macaw nestlings with positive findings were between 33 and 88 days old. These results indicate widespread dissemination of this pathogen in the two evaluated psittacine populations. No birds had clinical signs suggestive of chlamydiosis. To the best of our knowledge, this is the first report on C. psittaci in free-living Blue-fronted Amazon parrots and Hyacinth macaws in Brazil.

Ichnology of deglaciation deposits from the Upper Carboniferous Rio do Sul Formation (Itararé Group, Paraná Basin) at central-east Santa Catarina State (southern Brazil)

NASA Astrophysics Data System (ADS)

Lima, João Henrique Dobler; Netto, Renata Guimarães; Corrêa, Camila Graziele; Lavina, Ernesto Luiz Corrêa

2015-11-01

Trace fossil assemblages dominated by arthropod trackways are common in sediments deposited during the Late Paleozoic Ice Age. Ichnofaunas preserved in glacially-influenced sedimentary successions were previously reported from Paraná Basin in southern Brazil. The ichnofauna of the Rio do Sul Formation preserved in the rhythmites exposed in Trombudo Central quarries (Santa Catarina State, southern Brazil) is revised in this paper. Cruziana problematica, Diplichnites gouldi, Diplopodichnus biformis, Glaciichnium liebegastensis, Gluckstadtella elongata isp. nov., Helminthoidichnites tenuis, Mermia carickensis, Protovirgularia dichotoma, Treptichnus pollardi and Umfolozia sinuosa were recorded. Two trace fossil suites were recognized. The undermat miners suite is dominated by H. tenuis, indicating the presence of surface grazers (insect larvae, isopods and amphipods). C. problematica, D. gouldi and U. sinuosa dominate the overmat grazers suite, as result of displacement of terrestrial and aquatic arthropods. The integrated sedimentological and ichnological data from Trombudo Central region suggests colonization of ephemeral, shallow water bodies filled by freshwater from glacier melting. The deposition of the rhythmites took place in a glaciolacustrine context represented by shallow ponds in marginal marine settings.

Surveillance for yellow Fever virus in non-human primates in southern Brazil, 2001-2011: a tool for prioritizing human populations for vaccination.

PubMed

Almeida, Marco A B; Cardoso, Jader da C; Dos Santos, Edmilson; da Fonseca, Daltro F; Cruz, Laura L; Faraco, Fernando J C; Bercini, Marilina A; Vettorello, Kátia C; Porto, Mariana A; Mohrdieck, Renate; Ranieri, Tani M S; Schermann, Maria T; Sperb, Alethéa F; Paz, Francisco Z; Nunes, Zenaida M A; Romano, Alessandro P M; Costa, Zouraide G; Gomes, Silvana L; Flannery, Brendan

2014-03-01

In Brazil, epizootics among New World monkey species may indicate circulation of yellow fever (YF) virus and provide early warning of risk to humans. Between 1999 and 2001, the southern Brazilian state of Rio Grande do Sul initiated surveillance for epizootics of YF in non-human primates to inform vaccination of human populations. Following a YF outbreak, we analyzed epizootic surveillance data and assessed YF vaccine coverage, timeliness of implementation of vaccination in unvaccinated human populations. From October 2008 through June 2009, circulation of YF virus was confirmed in 67 municipalities in Rio Grande do Sul State; vaccination was recommended in 23 (34%) prior to the outbreak and in 16 (24%) within two weeks of first epizootic report. In 28 (42%) municipalities, vaccination began more than two weeks after first epizootic report. Eleven (52%) of 21 laboratory-confirmed human YF cases occurred in two municipalities with delayed vaccination. By 2010, municipalities with confirmed YF epizootics reported higher vaccine coverage than other municipalities that began vaccination. In unvaccinated human populations timely response to epizootic events is critical to prevent human yellow fever cases.

[The impact of low birth weight related to gestational depression on federal funding of public health: a study in Pelotas, Rio Grande do Sul State, Brazil].

PubMed

Menezes, Leticia Oliveira de; Pinheiro, Ricardo Tavares; Quevedo, Luciana de Avila; Oliveira, Sandro Schreiber de; Silva, Ricardo Azevedo da; Pinheiro, Karen Amaral Tavares; Santo, Graciela Coelho Espírito; Jansen, Karen

2012-10-01

Low birth weight is related to morbidity and mortality and sequelae during infant development, thereby impacting health system costs. It is thus important to evaluate factors that influence low birth weight and to estimate their impact on the Brazilian Unified National Health System (SUS). This was a nested prospective study in a cohort of pregnant women who received prenatal care and gave birth in the National Health System in hospitals with ICUs in the city of Pelotas, Rio Grande do Sul State, Brazil. Gestational depression was associated with a fourfold risk of low birth weight (PR = 3.94; CI: 1.49-10.36). Based on the population-attributable fraction, in the overall population an estimated 36.17% of low birth weight infants are born to mothers with an episode of depression during pregnancy, with an estimated cost of more than R$76 million (U$38 million) in Brazil. The study recommends the expansion of preventive and therapeutic mental health care measures for pregnant women and the adequate use of resources in the Unified National Health System to improve neonatal outcomes.

Surveillance for Yellow Fever Virus in Non-Human Primates in Southern Brazil, 2001–2011: A Tool for Prioritizing Human Populations for Vaccination

PubMed Central

Almeida, Marco A. B.; Cardoso, Jader da C.; dos Santos, Edmilson; da Fonseca, Daltro F.; Cruz, Laura L.; Faraco, Fernando J. C.; Bercini, Marilina A.; Vettorello, Kátia C.; Porto, Mariana A.; Mohrdieck, Renate; Ranieri, Tani M. S.; Schermann, Maria T.; Sperb, Alethéa F.; Paz, Francisco Z.; Nunes, Zenaida M. A.; Romano, Alessandro P. M.; Costa, Zouraide G.; Gomes, Silvana L.; Flannery, Brendan

2014-01-01

In Brazil, epizootics among New World monkey species may indicate circulation of yellow fever (YF) virus and provide early warning of risk to humans. Between 1999 and 2001, the southern Brazilian state of Rio Grande do Sul initiated surveillance for epizootics of YF in non-human primates to inform vaccination of human populations. Following a YF outbreak, we analyzed epizootic surveillance data and assessed YF vaccine coverage, timeliness of implementation of vaccination in unvaccinated human populations. From October 2008 through June 2009, circulation of YF virus was confirmed in 67 municipalities in Rio Grande do Sul State; vaccination was recommended in 23 (34%) prior to the outbreak and in 16 (24%) within two weeks of first epizootic report. In 28 (42%) municipalities, vaccination began more than two weeks after first epizootic report. Eleven (52%) of 21 laboratory-confirmed human YF cases occurred in two municipalities with delayed vaccination. By 2010, municipalities with confirmed YF epizootics reported higher vaccine coverage than other municipalities that began vaccination. In unvaccinated human populations timely response to epizootic events is critical to prevent human yellow fever cases. PMID:24625681

Cystic Fibrosis: Brazilian ENT Experience

PubMed Central

Sih, Tania; Godinho, Ricardo; Franco, Leticia Paiva; Piltcher, Otávio

2012-01-01

Most published studies about Cystic Fibrosis (CF) are European or North American. There are still few publications about the characteristics of fibrocystic populations in developing countries. The incidence of cystic fibrosis (CF) in Brazil varies among different regions (1 : 10,000 in Minas Gerais, 1 : 9,500 in Paraná, 1 : 8,700 in Santa Catarina, and 1 : 1600 in Rio Grande do Sul). The prevalence of the DF508 mutation also varies according to population: 33% in Sao Paulo, 49% in Rio Grande do Sul, 27% in Santa Catarina, and 52% in Minas Gerais. Cough and nasal obstruction are the most common symptoms. The variation in nasal polyposis prevalence may be explained by population genotypic characteristics in a country that spans a continent. Findings on nasal endoscopy and computed tomography (CT) have better correlation than do this information compared with surgical and clinical history. Microbiologic studies suggest a high level of early contamination of the airways. Sensorineural hearing loss (SNHL) occurs in these patients as a result of ototoxic antibiotics. The data compiled in this paper is useful, but also lead to the general agreement that more research would be welcome due to the unique characteristics of this country. PMID:22611403

Gastrointestinal parasites and prey items from a mass stranding of false killer whales, Pseudorca crassidens, in Rio Grande do Sul, Southern Brazil.

PubMed

Andrade, A L; Pinedo, M C; Barreto, A S

2001-02-01

The gastrointestinal tract of 14 false killer whales, 6 males and 8 females, stranded in June 1995 in southern Brazil, with total standard lengths from 338 to 507 cm, were analysed for endoparasites and food items. A pregnant female had a male foetus of 77.5 cm. Parasites were found in all 14 false killer whales. The nematode Anisakis simplex (Rudolphi, 1809) was found in the stomach of 57% of the animals and the acanthocephalan Bolbosoma capitatum (Linstow, 1889) Porta, 1908 was present in the intestine of all specimens and showed densities up to 600 m-1. An unidentified cestode (Tethrabothridae) was found also in the intestines of 14% of the individuals. The high infections of B. capitatum and A. simplex were not directly related with the cause of death. In the stomachs of four females, beaks of at least eight specimens of the oceanic and epipelagic species Ommastrephes bartramii (Lesueur, 1821) were found, with mantle lengths ranging from 189.8 to 360.9 mm. The distribution of O. bartramii in the coast of Rio Grande do Sul is consistent with false killer whales feeding in continental shelf waters.

Genetic characterization of cassava (Manihot esculenta) landraces in Brazil assessed with simple sequence repeats

PubMed Central

2009-01-01

Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava (Manihot esculenta) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves (HS = 0.52). Cluster analysis and principal component based scatter plotting showed greater similarity among landraces from São Paulo, Mato Grosso do Sul and Amazonas, whereas those from Minas Gerais were clustered into a sub-group within this group. The plants from Mato Grosso, mostly collected in the municipality of General Carneiro, provided the highest differentiation. The migration of human populations is one among the possible reasons for this closer resemblance or greater disparity among plants from the various regions. PMID:21637653

[Therapeutic itineraries of transvestites from the central region of the state of Rio Grande do Sul, Brazil].

PubMed

Souza, Martha Helena Teixeira de; Signorelli, Marcos Claudio; Coviello, Denise Martin; Pereira, Pedro Paulo Gomes

2014-07-01

The scope of this paper is to shed light on the therapeutic itineraries of transvestites from Santa Maria in the central region of the state of Rio Grande do Sul in southern Brazil. The study sought to follow the complex trajectories followed by transvestites in their quest for health care. Field research was conducted between January and November 2012 with transvestites from different cities in the state who were living in Santa Maria at the time. It involved qualitative methodology using ethnographic research. The results showed that the interviewees avoid institutionalized health services, opting for other forms of health care. In this respect, it is noteworthy that of the group of 49 transvestites who were included in this study, 48 sought health care in "African religion groups" or "batuque" ("drumming"), as they refer to them. The transvestites stated that they opted for "African religion groups" as they saw them as places that were able to afford forms of care and protection, without questioning bodily changes and sexual orientation. This article may help to shed light on some of the unusual trajectories of transvestites in their quest for health care.

Reconnaissance for radioactive materials in the southern part of Brazil

USGS Publications Warehouse

Pierson, Charles T.; Haynes, Donald D.; Filho, Evaristo Ribeiro

1957-01-01

During 1954-1956 a reconnaissance for radioactive minerals was made with carborne, airborne and handborne scintillation equipment in the southern Brazilian states of Rio de Janeiro, Sao Paulo, Parana, Santa Catarina and Rio Grande do Sul. During the traverse covering more than 5,000 kilometers the authors checked the radioactivity of Precambrian igneous and metamorphic rocks, Paleozoic, Mesozoic and Cenozoic sedimentary rocks, and Mesozoic alkalic intrusive and basaltic extrusive rocks. The 22 samples collected contained from 0.003 to 0.029 percent equivalent uranium oxide and from 0.10 to 0.91 percent equivalent thorimn; two samples were taken from radioactive pegmati tes for mineralogic studies. None of the localities is at present a commercial source of uranium or thorium; however, additional work should be done near the alkalic stock at Lages in the State of Santa Catarina and at the Passo das Tropas fossil plant locality near Santa Maria in the state of Rio Grande do Sul. Near Lages highly altered alkalic rock from a dike contained 0.026 percent uranium oxide. At Passo das Tropas highly altered, limonite-impregnated sandstone from the Rio do Rasto group of sedimentary rocks contained 0.029 percent uranium oxide.

[Trends and inequalities in risk behaviors among adolescents: a comparison of birth cohorts in Pelotas, Rio Grande do Sul State, Brazil].

PubMed

Arroyave, Leidy Johanna Ocampo; Restrepo-Méndez, María Clara; Horta, Bernardo Lessa; Menezes, Ana Maria Baptista; Gigante, Denise Petrucci; Gonçalves, Helen

2016-10-10

This study focuses on trends and inequalities in health risk behaviors among adolescents. A cross-sectional study compared two birth cohorts in the city of Pelotas, Rio Grande do Sul State, Brazil. The sample included 1,281 adolescents from the 1982 cohort and 4,106 from the 1993 cohort, followed in 2001 and 2011, respectively. The study recorded alcohol intake, illegal drug use, smoking, sexual initiation < 16 years, lack of condom use, and multiple sex partners. Total prevalence rates were calculated for each cohort, stratified by gender and per capita income, besides absolute and relative measures of inequality. There was a decrease from 2001 to 2011 in prevalence rates for trying alcohol, illegal drug use, smoking, and lack of condom use, and an increase in the number of sex partners. The gap between boys and girls increased for non-use of condoms and decreased for the other behaviors. The gap between income groups decreased for sexual initiation before 16 years of age and increased for episodes of intoxication. Socioeconomic inequalities persist, despite the downward trend in prevalence of risk behaviors.

Access to treatment for phenylketonuria by judicial means in Rio Grande do Sul, Brazil.

PubMed

Trevisan, Luciano Mangueira; Nalin, Tatiele; Tonon, Tassia; Veiga, Lauren Monteiro; Vargas, Paula; Krug, Bárbara Corrêa; Leivas, Paulo Gilberto Cogo; Schwartz, Ida Vanessa Doederlein

2015-05-01

Treatment of phenylketonuria (PKU) includes the use of a metabolic formula which should be provided free of charge by the Unified Health System (SUS). This retrospective, observational study sought to characterize judicial channels to obtain PKU treatment in Rio Grande do Sul (RS), Brazil. Lawsuits filed between 2001- 2010 and having as beneficiaries PKU patients requesting treatment for the disease were included. Of 20 lawsuits filed, corresponding to 16.8% of RS patients with PKU, 19 were retrieved for analysis. Of these, only two sought to obtain therapies other than metabolic formula. In all the other 17 cases, prior treatment requests had been granted by the State Department of Health. Defendants included the State (n = 19), the Union (n = 1), and municipalities (n = 4). In 18/19 cases, the courts ruled in favor of the plaintiffs. Violation of the right to health and discontinuation of State-provided treatment were the main reasons for judicial recourse. Unlike other genetic diseases, patients with PKU seek legal remedy to obtain a product already covered by the national pharmaceutical assistance policy, suggesting that management failures are a driving factor for judicialization in Brazil.

Serological Investigation into Paracoccidioides brasiliensis Infection in Dogs from Southern Rio Grande do Sul, Brazil.

PubMed

Teles, Alessandra Jacomelli; Klafke, Gabriel Baracy; Cabana, Ângela Leitzke; Albano, Ana Paula Neuschrank; Xavier, Melissa Orzechowski; Meireles, Mário Carlos Araújo

2016-04-01

Paracoccidioides brasiliensis is a dimorphic fungus and major cause of paracoccidioidomycosis (PCM), which is a systemic and endemic mycosis in Brazil. In Southern Brazil, an increased number of cases were detected since the 1990 s. Therefore, in order to determine areas with the presence of the fungus, this study aimed to investigate infection by P. brasiliensis in dogs from Southern Brazil. Indirect ELISA was used to detect antibodies against P. brasiliensis gp43. One hundred and ninety-six stray and semi-domiciled dogs from the municipalities of Pelotas and Capão do Leão, Rio Grande do Sul were included in this study. P. brasiliensis infection was detected in 58 animals (29.6%) with no significant difference for gender, age and breed. Seropositive animals were detected in all neighborhoods in the city of Pelotas as well as in the neighboring municipality Capão do Leão. The detection of antibodies against gp43 in dogs suggests the presence and wide distribution of the fungus in Pelotas and Capão do Leão, warning for the possibility of PCM disease in dogs as well as in humans from this region.

Risk factors and infection due to Cryptosporidium spp. in dogs and cats in southern Rio Grande do Sul.

PubMed

Moreira, Andrios da Silva; Baptista, Cristiane Telles; Brasil, Carolina Litchina; Valente, Júlia de Souza Silveira; Bruhn, Fábio Raphael Pascoti; Pereira, Daniela Isabel Brayer

2018-01-01

This study investigated the frequency of oocysts of Cryptosporidium spp. in feces from dogs and cats in five municipalities in the southern region of the state of Rio Grande do Sul. The risk factors associated with infection were also investigated. Feces samples from 110 dogs and 18 cats were stained using the auramine method. At the time of feces sampling, a questionnaire with semi-open-ended questions was applied to the animal guardians and all data obtained underwent statistical analysis. The real frequency of oocysts of Cryptosporidium spp. was 24.63% (27 dogs and two cats). Only four samples of dog feces were diarrheic and no presence of oocysts was observed in any of them. Variables that represented risk factors for infection were: homemade food, untreated water, circulation of animals on grassy terrain and living in the same environment as other animals (cattle). The results made it possible to inferring that within the population studied, the frequency of parasitism due to Cryptosporidium spp. in dogs was relevant and emphasize the asymptomatic nature of this infection. The adopting control measures are highlighted, particularly in relation to variables that represent risk factors for this infection.

[Seroepidemiology of Toxoplasma gondii in elderly individuals treated under the Family Health Strategy, Porto Alegre, Rio Grande do Sul, Brazil].

PubMed

Engroff, Paula; Ely, Luísa Scheer; Guiselli, Samilla Roversi; Goularte, Fabiana Henriques; Gomes, Irenio; Viegas, Karin; Carli, Geraldo Attilio De

2014-08-01

The aim of this study was to evaluate the seroprevalence of Toxoplasma gondii and relate it to the socioeconomic, hygienic, sanitary and health conditions of the elderly of the Family Health Strategy (FHS) in the city of Porto Alegre, Rio Grande do Sul, Brazil. The research involved a cross-sectional study in which a questionnaire with epidemiologic questions was applied and blood samples were taken. The assessment of IgG and IgM anti-T. gondii was performed using the ELISA technique. Seroprevalence was evaluated among 599 elderly individuals with 88% for IgG anti-T. gondii and with 0.8% for IgM. In the multivariate analysis, the variables that associated themselves independently with positive IgG were age range, personal income and wearing spectacles. Those associated with positive IgM were age, self-rated health and wearing spectacles. The results call attention to the high prevalence of IgG anti-T. gondii in elderly individuals in the FHS in Porto Alegre, generating concern in the event that the reactivation of toxoplasmosis and the development of more severe symptoms of this infection occur.

[Health on the borders: access to and demands on the Brazilian National Health System by foreigners and non-resident Brazilians in cities along the border with MERCOSUR countries from the perspective of municipal health secretaries].

PubMed

Giovanella, Ligia; Guimarães, Luisa; Nogueira, Vera Maria Ribeiro; Lobato, Lenaura de Vasconcelos Costa; Damacena, Giseli Nogueira

2007-01-01

In the context of forming common markets, border areas require special attention, since they anticipate the effects of integration processes. Along borders, different political, monetary, security, and social systems coexist; the intensification of flows resulting from integration raises challenges for the health systems, requiring specific policies focused on guaranteeing the right to health. This article presents the results of a study on the conditions for access to (and demands for) health services in the MERCOSUR border cities. A survey was performed with municipal health secretaries in the 69 Brazilian cities in the States of Rio Grande do Sul, Santa Catarina, Paraná, and Mato Grosso do Sul that border on the other MERCOSUR countries. The study attempted to identify the services demanded by the border population, mechanisms used for access, flows between services and systems, response strategies, and local agreements. Initiatives for cooperation between Brazilian and foreign local administrators were identified in nearly half of the municipalities and can orient the formulation of guidelines for border situations, allowing improvement in comprehensive access to health care.

Engineering the bacterial shapes for enhanced inclusion bodies accumulation.

PubMed

Jiang, Xiao-Ran; Wang, Huan; Shen, Rui; Chen, Guo-Qiang

2015-05-01

Many bacteria can accumulate inclusion bodies such as sulfur, polyphosphate, glycogen, proteins or polyhydroxyalkanoates. To exploit bacteria as factories for effective production of inclusion bodies, a larger intracellular space is needed for more inclusion body accumulation. In this study, polyhydroxybutyrate (PHB) was investigated as an inclusion bodies representative to be accumulated by Escherichia coli JM109SG. Various approaches were taken to increase the bacterial cell sizes including deletion on actin-like protein gene mreB, weak expression of mreB in mreB deletion mutant, and weak expression of mreB in mreB deletion mutant under inducible expression of SulA, the inhibitor of division ring protein FtsZ. All of the methods resulted in different levels of increases in bacterial sizes and PHB granules accumulation. Remarkably, an increase of over 100% PHB accumulation was observed in recombinant E. coli overexpressing mreB in an mreB deletion mutant under inducible expression of FtsZ inhibiting protein SulA. The molecular mechanism of enlarged bacterial size was found to be directly relate to weakened cytoskeleton which was the result of broken skeleton helix. Copyright © 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Evaluation of the Efficiency of Selective Collection in a Small Town on the State of Rio Grande do Sul - Brazil

NASA Astrophysics Data System (ADS)

Schneider, V. E.; Poletto, M.; Peresin, D.; Carra, S. H. Z.; Vanni, D.

2017-07-01

With the increase of population concentration in urban areas, there is an increase in the solid waste generation, which demands the search for alternatives and solutions for the environmentally correct destination of these. In this context, this work presents an evaluation on the forms of organic and selective domestic waste collection and the potential for the recyclability of the waste destined to the same, based on the physical characterization and gravimetric composition of the solid wastes generated in the town of Antônio Prado, located in the state of Rio Grande do Sul, Brazil, between 2014 and 2016. It is observed that the population has significant information regarding the correct disposal of waste in the selective collection, since 60% of the waste destined to the same is effectively recyclable. Plastic (24.8%), paper (10.9%), glass (8.8%) and cardboard (8.4%) are the most representative materials in recycled waste samples in the urban area. The importance of continuity and improvement of environmental education programs is essential, due to the evolution in the quantity and complexity of products and materials currently manufactured, and to the method of mechanized waste collection used by the municipality.

Hemoparasites of the genus Trypanosoma (Kinetoplastida: Trypanosomatidae) and hemogregarines in Anurans of the São Paulo and Mato Grosso do Sul States - Brazil.

PubMed

Leal, Denise D M; O'dwyer, Lucia H; Ribeiro, Vitor C; Silva, Reinaldo J; Ferreira, Vanda L; Rodrigues, Rozangela B

2009-06-01

Wild animals are exposed to numerous pathogens, including hemoparasites. The Trypanosoma and hemogregarinegroup are frequently reported as parasites in anurans (frogs, tree frogs and toads). The identification of these hemoparasites is usually made through stage observation of their morphology in the peripheral blood of the host. There areno studies, however, based on the biological cycle of these hemoparasites. The objective of the present study was toevaluate the presence of hemogregarines and Trypanosoma spp. in anurans captured in the States of São Paulo andMato Grosso do Sul- Brazil and to perform the morphological and morphometric characterization of these hemoparasites. The species of anurans examined were: Dendropsophus nanus, D. minutus, Leptodactylus chaquensis L. podicipinus, L. labyrinthicus, L. fuscus, Bufo granulosus, B. schneideri, Phyllomedusa hypocondrialis, Trachicephalus venulosus, Scinax fuscovarius and Hypsiboas albopunctatus. Of the total of 40 animals studied, four (10%)were positive for hemogregarines and eight (20%) were positive for Trypanosoma spp. Hemogregarine gamontsshowed variable morphology and, in addition to intraerythrocytic forms, extraerythrocytic forms were also observed.Extremely different forms of Trypanosoma were observed, as described in the literature, with the broad and oval forms being the most common.

Insects galls of Pantanal areas in the State of Mato Grosso do Sul, Brazil: characterization and occurrence.

PubMed

Ascendino, Sharlene; Maia, Valéria C

2018-01-01

Two Pantanal areas were investigated in Corumbá, Mato Grosso do Sul. The field works were carried out in five trails, in August and December/2011 and April/2012. Seventy-six gall morphotypes were found in 52 host plants, 22 of them were identified at species level and 30 at genus. Fabaceae, Myrtaceae and Sapindaceae were the plant families with the greatest richness of gall. Leaves were the plant organ with the greatest gall richness. Six gall shapes were found - globoid, lenticular, fusiform, cylindrical, conical, and marginal roll. The three first were the most common. Five colors were observed - green, brown, red, yellow, and black. Green and brown predominated. Most were glabrous and one-chambered. These features have been indicated in several Brazilian biomes. Diptera (Cecidomyiidae) were the most frequent gallers. The associated fauna comprised parasitoids (Eulophidae, Braconidae, Eupelmidae, Eurytomidae, Platygastridae, and Pteromalidae), inquilines (Cecidomyiidae: Trotteria Kieffer, 1902, Hymenoptera: Tanaostigmoides Ashmead, 1896, Hemiptera, Lepidoptera and Thysanoptera) and predators (Pseudoscorpionida: Olpiolum medium Beier, 1931, Cecidomyiidae: Lestodiplosini and Hymenoptera: Formicidae). Parasitoids were the most frequent. Multiparatism was observed. Our data represent the first contribution to the knowledge of the associated fauna in the Pantanal.

Fate of tetracycline, sulfonamide and fluoroquinolone resistance genes and the changes in bacterial diversity during composting of swine manure.

PubMed

Selvam, Ammaiyappan; Xu, Delin; Zhao, Zhenyong; Wong, Jonathan W C

2012-12-01

This study monitored the abundance of antibiotic resistant genes (ARGs) and the bacterial diversity during composting of swine manure spiked with chlortetracycline, sulfadiazine and ciprofloxacin at two different levels and a control without antibiotics. Resistance genes of tetracycline (tetQ, tetW, tetC, tetG, tetZ and tetY), sulfonamide (sul1, sul2, dfrA1 and dfrA7) and fluoroquinolone (gyrA and parC) represented 0.02-1.91%, 0.67-10.28% and 0.00005-0.0002%, respectively, of the total 16S rDNA copies in the initial composting mass. After 28-42 days of composting, these ARGs, except parC, were undetectable in the composting mass indicating that composting is a potential method of manure management. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of bacterial 16S rDNA of the composting mass indicated that the addition of antibiotics up to 100, 20 and 20mg/kg of chlortetracycline, sulfadiazine and ciprofloxacin, respectively, elicited only a transient perturbation and the bacterial diversity was restored in due course of composting. Copyright © 2012 Elsevier Ltd. All rights reserved.

Profile and scientific production of CNPq researchers in Nephrology and Urology.

PubMed

Oliveira, Eduardo A; Pécoits-Filho, Roberto; Quirino, Isabel G; Oliveira, Maria Christina; Martelli, Daniela Reis; Lima, Leonardo S; Martelli, Hercílio

2011-03-01

This study aimed at evaluating the profile and scientific production of researchers in Nephrology and Urology, receiving grants in the area of Clinical Medicine from the Brazilian National Research Council. The standardized online curriculum vitae (Curriculum Lattes) of 39 researchers in Medicine receiving grants in the 2006-2008 triennium were included in the analysis. The variables analyzed were: gender, affiliation, time from completion of the PhD program, scientific production, and supervision of undergraduate students, and master's and PhD programs. Males (74.4%) and category 2 grants (56.4%) predominated. The following three Brazilian states are responsible for 90% of the researchers: São Paulo (28; 71.8%); Rio Grande do Sul (4; 10.3%); and Minas Gerais (3; 7.7%). Four institutions are responsible for 70% of the researchers: UNIFESP (14; 36%); USP (8; 20.5%); UFMG (3, 7.7%); and UNICAMP (3; 7.7%). Considering the academic career, the assessed researchers published 3,195 articles in medical journals, with a median of 75 articles per researcher (QI = 52-100). The researchers received a total of 25,923 citations at the database Web of Science®, with a median of 452 citations per researcher (QI = 161-927). The average number of citations per article was 13.8 citations (SD = 11.6). The Southeastern region of Brazil concentrates researchers in Nephrology and Urology. Our study has shown an increase in the scientific production of most researchers in the last five years. By knowing the profile of researchers in Nephrology and Urology, more effective strategies to encourage the scientific production and the demand for resources to finance research projects can be defined.

Attitudine Linguistica e Memoria. Alcune Considerazioni sul MLAT. (Modern Language Aptitude Test). (Linguistic Aptitude and Memory. Some Considerations on the MLAT).

ERIC Educational Resources Information Center

Sorace, Antonella

1982-01-01

Examines the Modern Language Aptitude Test and identifies as the lowest common denominator in three of its four parts an individual's short-term Memory capability. Concludes that this test cannot indicate an individual's linguistic aptitude because it does not take into consideration the role of two key aspects of language learning: long-term…

Howitzer Ammunition System Procurement (HASP).

DTIC Science & Technology

1991-07-01

machine tools , etc.) * Most critical part of base to reassemble. IPP * Industry to plan round-specific...beyond allowed tolerances. - Conducting tolerance studies and funding machining studies at sul’on "’actors. " Facility development was controlled by the...Manufacturing Balimoy Mfg. of Venice, Inc. Action Manufacturing Co. Lanson Industries Inc. Hercules Aerospace Company CIMA Machine & Tool Co., Inc. Talley Defense Systems Tracor Aerospace Inc. BMY E49030APPBMAC

Corrosion Fatigue

DTIC Science & Technology

1981-10-01

particularly under conditions of cathodic polarization. Sul- fate ion , while less damaging under free corrosion conditions, is equally aggressive at...Editing and Reproduction Ltd Harford 11ouse, 7-9 Charlotte St, London, WIP 1HD I I - PREFACE Failure by fatigue and degradation by corrosion continue to...of halide ions . In the unstressed state, this degrada- tion may be manifested by localized corrosion such as pitting, crevice corrosion or ex

A new species of Rhodnius from Brazil (Hemiptera, Reduviidae, Triatominae)

PubMed Central

da Rosa, João Aristeu; Justino, Hernany Henrique Garcia; Nascimento, Juliana Damieli; Mendonça, Vagner José; Rocha, Claudia Solano; de Carvalho, Danila Blanco; Falcone, Rossana; Oliveira, Maria Tercília Vilela de Azeredo; Alevi, Kaio Cesar Chaboli; de Oliveira, Jader

2017-01-01

Abstract A colony was formed from eggs of a Rhodnius sp. female collected in Taquarussu, Mato Grosso do Sul, Brazil, and its specimens were used to describe R. taquarussuensis sp. n. This species is similar to R. neglectus, but distinct characters were observed on the head, thorax, abdomen, female external genitalia and male genitalia. Chromosomal differences between the two species were also established. PMID:28769676

40 CFR 721.1550 - Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1).

Code of Federal Regulations, 2010 CFR

2010-07-01

...)(vi), (a)(5)(vii), (a)(6)(i), (a)(6)(ii), and (c). (ii) Hazard communication program. Requirements as...). In addition, the following human health hazard statement shall appear on each label as specified at... they are true and do not alter the meaning of the required statement. Human health hazard statements...

Improvement of the efficient referencing and sample positioning system for micro focused synchrotron X-ray techniques

NASA Astrophysics Data System (ADS)

Spangenberg, T.; Göttlicher, J.; Steininger, R.

2016-05-01

An efficient referencing and sample positioning system is a basic tool for a micro focus beamline at a synchrotron. The seven years ago introduced command line based system was upgraded at SUL-X beamline at ANKA [1]. A new combination of current server client techniques offers direct control and facilitates unexperienced users the handling of this frequently used tool.

Molecular Basis of Sulfonamide and Trimethoprim Resistance in Fish-Pathogenic Aeromonas Isolates ▿

PubMed Central

Kadlec, Kristina; von Czapiewski, Ellen; Kaspar, Heike; Wallmann, Jürgen; Michael, Geovana Brenner; Steinacker, Ulrike; Schwarz, Stefan

2011-01-01

Sulfonamide-trimethoprim-resistant Aeromonas salmonicida and motile Aeromonas spp. from diseased fish of the GERM-Vet study carried the sul1 gene together with mostly cassette-borne trimethoprim resistance genes, including the novel gene dfrA28. The seven dfrA and dfrB genes identified were located mostly in class 1 integrons which commonly harbored other gene cassettes. PMID:21764945

Proceedings of the Fourth Symposium on Conference on Dynamics of Cosmic Gases, Varenna Italy (Lake of Como), August 18-30, 1960; ATTI DEL IV SYMPOSIUM SULLA DINAMICA DEL GAS COSMICO, VARENNA SUL LAGO DI COMO 18-30 AGOSTO 1960

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1961-01-01

Fifteen papers are included: separate abstracts have been prepared for l0. A single abstract has been prepared to cover the remaining 5 papers, which consist of observational studies. Extensive discussions are included. (T.F.H.)

On the seismic behavior of the main tower of the San Felice sul Panaro (Italy) fortress

NASA Astrophysics Data System (ADS)

Castellazzi, Giovanni; D'Altri, Antonio Maria; de Miranda, Stefano; Magagnini, Stefano; Tralli, Antonio

2016-12-01

The medieval fortresses are a very common and distinctive type among the Emilian historical constructions and the earthquakes of May 20th and 29th, 2012 underlined their high vulnerability. Among those heavily damaged, there is the fortress of San Felice sul Panaro located between the two epicenters. This study presents some FE results regarding the behavior under seismic actions of the main tower (Mastio tower). The Mastio has peculiar geometric features and represents a typical example of non-isolated tower. In fact, it is constrained in very different ways by the surrounding parts of the fortress along two of its sides: on the north side it is constrained by the perimeter wall until one third of his high, while a stiffer building constrains it on the west side. In order to remodel the entire fortress, a multidisciplinary project involving the Municipality of San Felice sul Panaro and four Universities of the Emilia- Romagna (Bologna, Ferrara, Parma and Modena) together with the University of Genoa is going on. The study, oriented to the structural restoration, produced an accurate survey of the entire building including a fine definition of architectural peculiarities, historical stages and materials evolution. Based on such geometrical data, we developed a detailed 3D realistic mesh, with a point-by-point characterization of each single geometric element. We performed both pushover and nonlinear dynamic analyses using accelerograms data measured near the fortress on May 29th. A damage-plasticity material model exhibiting softening in both tension and compression, already available in the commercial code Abaqus, has been used for masonry in nonlinear dynamic analyses. On the other hand, pushover analyses have been performed utilizing similar constitutive equations available on code DIANA. The effects of higher modes of vibration have been taken into account by means of the modal pushover analysis technique. For the sake of conciseness, only some preliminary findings are presented in this paper. The results obtained with pushover analyses fit reasonably well with nonlinear dynamic simulations.

Prevalence of antibiotic resistance genes from effluent of coastal aquaculture, South Korea.

PubMed

Jang, Hyun Min; Kim, Young Beom; Choi, Sangki; Lee, Yunho; Shin, Seung Gu; Unno, Tatsuya; Kim, Young Mo

2018-02-01

The wide use of antibiotics in aquaculture for prophylactic and therapeutic purposes can potentially lead to the prevalence of antibiotic resistance genes (ARGs). This study reports for the first time the profile of ARGs from effluents of coastal aquaculture located in South Jeolla province and Jeju Island, South Korea. Using quantitative PCR (qPCR), twenty-two ARGs encoding tetracycline resistance (tetA, tetB, tetD, tetE, tetG, tetH, tetM, tetQ, tetX, tetZ, tetBP), sulfonamide resistance (sul1, sul2), quinolone resistance (qnrD, qnrS, aac(6')-Ib-cr), β-lactams resistance (bla TEM , bla CTX , bla SHV ), macrolide resistance (ermC), florfenicol resistance (floR) and multidrug resistance (oqxA) and a class 1 integrons-integrase gene (intI1) were quantified. In addition, Illumina Miseq sequencing was applied to investigate microbial community differences across fish farm effluents. Results from qPCR showed that the total number of detected ARGs ranged from 4.24 × 10 -3 to 1.46 × 10 -2 copies/16S rRNA gene. Among them, tetB and tetD were predominant, accounting for 74.8%-98.0% of the total ARGs. Furthermore, intI1 gene showed positive correlation with tetB, tetD, tetE, tetH, tetX, tetZ tetQ and sul1. Microbial community analysis revealed potential host bacteria for ARGs and intI1. Two genera, Vibrio and Marinomonas belonging to Gammaproteobacteria, showed significant correlation with tetB and tetD, the most dominant ARGs in all samples. Also, operational taxonomic units (OTUs)-based network analysis revealed that ten OTUs, classified into the phyla Proteobacteria, Cyanobacteria/Chloroplast, Bacteroidetes, Verrucomicrobia and an unclassified phylum, were potential hosts of tetracycline resistance genes (i.e., tetA, tetG, tetH, tetM, tetQ and tetZ). Further systematic monitoring of ARGs is warranted for risk assessment and management of antibacterial resistance from fish farm effluents. Copyright © 2017 Elsevier Ltd. All rights reserved.

Prediction of Sustained Virological Response to Peginterferon-based Therapy for Chronic Hepatitis C: Regression Analysis of a Cohort from Rio Grande do Sul, Brazil

PubMed Central

Fendt, Lúcia; Amaral, Karine; D Picon, Paulo

2017-01-01

Aim: Peginterferon plus ribavirin (peg-IFN/RBV) is still the standard of care for treatment of hepatitis C virus (HCV) in many countries. Given the high toxicity of this regimen, our study aimed to develop a prediction tool that can identify which patients are unlikely to benefit from peg-IFN/RBV and could thus postpone treatment in favor of new-generation direct-acting antivirals. Materials and methods: Binary regression was performed using demographic, clinical, and laboratory covariates and sustained virological response (SVR) outcomes from a prospective cohort of individuals referred for therapy from 2003 to 2008 in a public HCV treatment center in Rio Grande do Sul, Brazil. Results: Of the 743 participants analyzed, 489 completed 48 weeks of treatment (65.8%). A total of 202 of those who completed peg-IFN/RBV therapy achieved SVR (27.2% responders), 196 did not (26.4%), and 91 had missing viral load (VL) at week 72 (12.2% loss to follow-up). The remainder discontinued therapy (n = 254 [34.2%]), 78 (30.7%) doing so due to adverse effects. Baseline covariates included in the regression model were sex, age, human immunodeficiency virus, infection status, aspartate transaminase, alanine transaminase, hemoglobin, platelets, serum creatinine, prothrombin time, pretreatment VL, cirrhosis on liver biopsy, and treatment naivety. A predicted SVR of 17.9% had 90.0% sensitivity for detecting true nonresponders. The negative likelihood ratio at a predicted SVR of 17.9% was 0.16, and the negative predictive value was 92.6%. Conclusion: Easily obtainable variables can identify patients that will likely not benefit from peg-IFN-based therapy. This prediction model might be useful to clinicians. Clinical significance: To our knowledge, this is the only prediction tool that can reliably help clinicians to postpone peg-IFN/RBV therapy for HCV genotype 1 patients. How to cite this article: Picon RV, Fendt L, Amaral K, Picon PD. Prediction of Sustained Virological Response to Peginterferon-based Therapy for Chronic Hepatitis C: Regression Analysis of a Cohort from Rio Grande do Sul, Brazil. Euroasian J Hepato-Gastroenterol 2017;7(1):27-33. PMID:29201768

Prediction of Sustained Virological Response to Peginterferon-based Therapy for Chronic Hepatitis C: Regression Analysis of a Cohort from Rio Grande do Sul, Brazil.

PubMed

V Picon, Rafael; Fendt, Lúcia; Amaral, Karine; D Picon, Paulo

2017-01-01

Peginterferon plus ribavirin (peg-IFN/RBV) is still the standard of care for treatment of hepatitis C virus (HCV) in many countries. Given the high toxicity of this regimen, our study aimed to develop a prediction tool that can identify which patients are unlikely to benefit from peg-IFN/RBV and could thus postpone treatment in favor of new-generation direct-acting antivirals. Binary regression was performed using demographic, clinical, and laboratory covariates and sustained virological response (SVR) outcomes from a prospective cohort of individuals referred for therapy from 2003 to 2008 in a public HCV treatment center in Rio Grande do Sul, Brazil. Of the 743 participants analyzed, 489 completed 48 weeks of treatment (65.8%). A total of 202 of those who completed peg-IFN/RBV therapy achieved SVR (27.2% responders), 196 did not (26.4%), and 91 had missing viral load (VL) at week 72 (12.2% loss to follow-up). The remainder discontinued therapy (n = 254 [34.2%]), 78 (30.7%) doing so due to adverse effects. Baseline covariates included in the regression model were sex, age, human immunodeficiency virus, infection status, aspartate transaminase, alanine transaminase, hemoglobin, platelets, serum creatinine, prothrombin time, pretreatment VL, cirrhosis on liver biopsy, and treatment naivety. A predicted SVR of 17.9% had 90.0% sensitivity for detecting true nonresponders. The negative likelihood ratio at a predicted SVR of 17.9% was 0.16, and the negative predictive value was 92.6%. Easily obtainable variables can identify patients that will likely not benefit from peg-IFN-based therapy. This prediction model might be useful to clinicians. To our knowledge, this is the only prediction tool that can reliably help clinicians to postpone peg-IFN/RBV therapy for HCV genotype 1 patients. How to cite this article: Picon RV, Fendt L, Amaral K, Picon PD. Prediction of Sustained Virological Response to Peginterferon-based Therapy for Chronic Hepatitis C: Regression Analysis of a Cohort from Rio Grande do Sul, Brazil. Euroasian J Hepato-Gastroenterol 2017;7(1):27-33.

Macrophytes may not contribute significantly to removal of nutrients, pharmaceuticals, and antibiotic resistance in model surface constructed wetlands.

PubMed

Cardinal, Pascal; Anderson, Julie C; Carlson, Jules C; Low, Jennifer E; Challis, Jonathan K; Beattie, Sarah A; Bartel, Caitlin N; Elliott, Ashley D; Montero, Oscar F; Lokesh, Sheetal; Favreau, Alex; Kozlova, Tatiana A; Knapp, Charles W; Hanson, Mark L; Wong, Charles S

2014-06-01

Outdoor shallow wetland mesocosms, designed to simulate surface constructed wetlands to improve lagoon wastewater treatment, were used to assess the role of macrophytes in the dissipation of wastewater nutrients, selected pharmaceuticals, and antibiotic resistance genes (ARGs). Specifically, mesocosms were established with or without populations of Typha spp. (cattails), Myriophyllum sibiricum (northern water milfoil), and Utricularia vulgaris (bladderwort). Following macrophyte establishment, mesocosms were seeded with ARG-bearing organisms from a local wastewater lagoon, and treated with a single pulse of artificial municipal wastewater with or without carbamazepine, clofibric acid, fluoxetine, and naproxen (each at 7.6μg/L), as well as sulfamethoxazole and sulfapyridine (each at 150μg/L). Rates of pharmaceutical dissipation over 28d ranged from 0.073 to 3.0d(-1), corresponding to half-lives of 0.23 to 9.4d. Based on calculated rate constants, observed dissipation rates were consistent with photodegradation driving clofibric acid, naproxen, sulfamethoxazole, and sulfapyridine removal, and with sorption also contributing to carbamazepine and fluoxetine loss. Of the seven gene determinants assayed, only two genes for both beta-lactam resistance (blaCTX and blaTEM) and sulfonamide resistance (sulI and sulII) were found in sufficient quantity for monitoring. Genes disappeared relatively rapidly from the water column, with half-lives ranging from 2.1 to 99d. In contrast, detected gene levels did not change in the sediment, with the exception of sulI, which increased after 28d in pharmaceutical-treated systems. These shallow wetland mesocosms were able to dissipate wastewater contaminants rapidly. However, no significant enhancement in removal of nutrients or pharmaceuticals was observed in mesocosms with extensive aquatic plant communities. This was likely due to three factors: first, use of naïve systems with an unchallenged capacity for nutrient assimilation and contaminant removal; second, nutrient sequestration by ubiquitous filamentous algae; and third, dominance of photolytic processes in the removal of pharmaceuticals, which overshadowed putative plant-related processes. Copyright © 2014 Elsevier B.V. All rights reserved.

Antimicrobial Resistance and Molecular Characterization of Salmonella enterica Serovar Enteritidis from Retail Chicken Products in Shanghai, China.

PubMed

Zhou, Xiujuan; Xu, Li; Xu, Xuebin; Zhu, Yuding; Suo, Yujuan; Shi, Chunlei; Shi, Xianming

2018-05-30

Salmonella enterica serovar Enteritidis is the leading global cause of salmonellosis. A total of 146 Salmonella Enteritidis isolates obtained from retail chicken products in Shanghai, China were characterized for their antimicrobial susceptibilities, virulence and antibiotic resistance gene profiles, and molecular subtypes using pulsed-field gel electrophoresis (PFGE). Approximately 42% (61/146) of the isolates were susceptible to all 13 antimicrobials tested. More than half of the isolates (50.70%) were resistant to ampicillin, 49.32% to sulfisoxazole, 17.12% to tetracycline, and 15.75% to doxycycline. Thirty (20.55%) isolates were resistant to three or more antimicrobials. The avrA, mgtC, and sopE virulence genes were identified in all isolates, while 97.2% and 92.4% were positive for bcfC and spvC genes, respectively. Genes associated with resistance to streptomycin (aadA), β-lactams (blaTEM, blaCMY, blaSHV, and blaCTX), tetracycline (tetA and tetB), and sulfonamides (sulI, sulII, and sulIII) were detected among corresponding resistant isolates. A total of 41 PFGE patterns were identified from 77 antimicrobial resistance (AMR) isolates and were primarily grouped into seven clusters (A-G), each with 90% similarity. The majority of Salmonella Enteritidis isolates (63.63%, 49/77) shared the same PFGE cluster, indicating potential cross contamination during processing and cutting or working during retailing and marketing. A significantly (p < 0.05) lower percentage (<25%) of isolates belonging to clusters D and E were resistant to sulfisoxazole compared with those belonging to clusters A, B, C, F, and G (>80%), indicating that sulfisoxazole resistance might be associated with genetic content (PFGE profiles) of Salmonella Enteritidis. This study provides important and updated information about the baseline antimicrobial-resistant data for food safety risk assessment of Salmonella Enteritidis from retailed chicken in Shanghai, which is the first step for the development and implementation of China's AMR National Action Plan, and can be helpful for future surveillance activities to ensure the safety of the chicken supply.

H2S Injection and Sequestration into Basalt - The SulFix Project

NASA Astrophysics Data System (ADS)

Gudbrandsson, S.; Moola, P.; Stefansson, A.

2014-12-01

Atmospheric H2S emissions are among major environmental concern associated with geothermal energy utilization. It is therefore of great importance for the geothermal power sector to reduce H2S emissions. Known solutions for H2S neutralization are both expensive and include production of elemental sulfur and sulfuric acid that needs to be disposed of. Icelandic energy companies that utilize geothermal power for electricity production have decided to try to find an environmentally friendly and economically feasible solution to reduce the H2S emission, in a joint venture called SulFix. The aim of SulFix project is to explore the possibilities of injecting H2S dissolved in water into basaltic formations in close proximity to the power plants for permanent fixation as sulfides. The formation of sulfides is a natural process in geothermal systems. Due to basalt being rich in iron and dissolving readily at acidic conditions, it is feasible to re-inject the H2S dissolved in water, into basaltic formations to form pyrite. To estimate the mineralization rates of H2S, in the basaltic formation, flow through experiments in columns were conducted at various H2S concentrations, temperatures (100 - 240°C) and both fresh and altered basaltic glass. The results indicate that pyrite rapidly forms during injection into fresh basalt but the precipiation in altered basalt is slower. Three different alteration stages, as a function of distance from inlet, can be observed in the column with fresh basaltic glass; (1) dissolution features along with precipitation, (2) precipitation increases, both sulfides and other secondary minerals and (3) the basalt looks to be unaltered and little if any precipitation is observed. The sulfur has precipitated in the first half of the column and thereafter the solution is possibly close to be supersaturated with respect to the rock. These results indicate that the H2S sequestration into basalt is possible under geothermal conditions. The rate limiting step is the availability of iron released from the dissolving rock. The rapid precipitation of secondary phases in the column suggests the possibility of decreased porosity in the vicinity of the injection well.

Longitudinal characterization of antimicrobial resistance genes in feces shed from cattle fed different subtherapeutic antibiotics

PubMed Central

2011-01-01

Background Environmental transmission of antimicrobial-resistant bacteria and resistance gene determinants originating from livestock is affected by their persistence in agricultural-related matrices. This study investigated the effects of administering subtherapeutic concentrations of antimicrobials to beef cattle on the abundance and persistence of resistance genes within the microbial community of fecal deposits. Cattle (three pens per treatment, 10 steers per pen) were administered chlortetracycline, chlortetracycline plus sulfamethazine, tylosin, or no antimicrobials (control). Model fecal deposits (n = 3) were prepared by mixing fresh feces from each pen into a single composite sample. Real-time PCR was used to measure concentrations of tet, sul and erm resistance genes in DNA extracted from composites over 175 days of environmental exposure in the field. The microbial communities were analyzed by quantification and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S-rRNA. Results The concentrations of 16S-rRNA in feces were similar across treatments and increased by day 56, declining thereafter. DGGE profiles of 16S-rRNA differed amongst treatments and with time, illustrating temporal shifts in microbial communities. All measured resistance gene determinants were quantifiable in feces after 175 days. Antimicrobial treatment differentially affected the abundance of certain resistance genes but generally not their persistence. In the first 56 days, concentrations of tet(B), tet(C), sul1, sul2, erm(A) tended to increase, and decline thereafter, whereas tet(M) and tet(W) gradually declined over 175 days. At day 7, the concentration of erm(X) was greatest in feces from cattle fed tylosin, compared to all other treatments. Conclusion The abundance of genes coding for antimicrobial resistance in bovine feces can be affected by inclusion of antibiotics in the feed. Resistance genes can persist in feces from cattle beyond 175 days with concentrations of some genes increasing with time. Management practices that accelerate DNA degradation such as frequent land application or composting of manure may reduce the extent to which bovine feces serves as a reservoir of antimicrobial resistance. PMID:21261985

Genetic characterization of antibiotic resistance in enteropathogenic Escherichia coli carrying extended-spectrum beta-lactamases recovered from diarrhoeic rabbits.

PubMed

Poeta, P; Radhouani, H; Gonçalves, A; Figueiredo, N; Carvalho, C; Rodrigues, J; Igrejas, G

2010-05-01

A total of 52 Escherichia coli strains isolated from diarrhoeic rabbits were investigated for their enteropathogenic E. coli (EPEC) pathotype by PCR amplification of eae and bfp virulence genes. A total of 22 EPEC isolates were identified, serotyped and studied for antibiotic resistance and screened for the detection of extended-spectrum beta-lactamases (ESBLs). The EPEC isolates belonged to three serogroups (O26, O92 and O103). The most common serogroup (O103:K-:H2) was observed among 17 EPEC strains, the O92:K-serogroup in three isolates (the antibiotic sensitive ones) and the remaining O26:K-serogroup in two isolates (the ESBLs isolates). Resistances to ampicillin and tetracycline were the most frequent and detected followed by resistance to nalidixic acid, streptomycin, trimethoprim-sulphamethoxazole, cefoxitin, gentamicin and ciprofloxacin. All the isolates were sensitive for amikacin, ceftazidime, aztreonam, imipenem, chloramphenicol, tobramycin and amoxicillin + clavulanic acid. Two isolates recovered from two adult animals showed an intermediate susceptibility to cefotaxime, and a positive screening test for ESBL was demonstrated in both. The bla(TEM) gene was demonstrated in the majority of ampicillin-resistant isolates. The aac(3)-II or aac(3)-IV genes were detected in the four gentamicin-resistant isolates. In addition, the aadA gene was detected in 60% of streptomycin-resistant isolates. The tet(A) or tet(B) genes were identified in all tetracycline-resistant isolates. A total of nine EPEC isolates showed the phenotype SXT-resistant, and the sul1 and/or sul2 and/or sul3 genes were detected in all of them. Our findings showed that the molecular detection by the eae and bfp genes by PCR followed by serotyping is useful for monitoring trends in EPEC infections of rabbits allowing the identification of their possible reservoirs. The detection of genes involved in the resistance to antibiotics of different families in a relatively high proportion of faecal E. coli isolates of rabbits is of great interest and could be considered a serious public health problem.

Using Real-Time Weather Data from an Unmanned Aircraft System to Support the Advanced Research Version of the Weather Research and Forecast Model

DTIC Science & Technology

2012-04-01

east central region of the domain, the Rio Grande River and Rio Grande valley are captured by the lowest contoured elevation region running NW-to...ARMY CECRLCECRL GP ATTN DR DETSCH HANOVER NH 03755-1290 1 CD USAF ROME LAB TECH CORRIDOR W STE 262 RL SUL 26 ELECTR PKWY BLD 106 GRIFFISS

Twenty-Five Year Epidemiology of Invasive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates Recovered at a Burn Center

DTIC Science & Technology

2009-01-01

elements and tend to carry resis- tance determinants in addition to mecA. In addition to varying antimicrobial resistance, the presence of certain...Antimicrobial susceptibility testing BMD testing was performed using CLSI criteria to determine antimicrobial susceptibility, broth microdilution performed...minocycline, trimethoprim –sul- famethoxazole, ciprofloxacin, rifampin, tigecycline and gen- tamicin. Wells containing daptomycin were supplemented with 50 mg

Predicting the geographic distribution of Lutzomyia longipalpis (Diptera: Psychodidae) and visceral leishmaniasis in the state of Mato Grosso do Sul, Brazil

PubMed Central

de Almeida, Paulo Silva; Sciamarelli, Alan; Batista, Paulo Mira; Ferreira, Ademar Dimas; Nascimento, João; Raizer, Josué; Andrade, José Dilermando; Gurgel-Gonçalves, Rodrigo

2013-01-01

To understand the geographic distribution of visceral leishmaniasis (VL) in the state of Mato Grosso do Sul (MS), Brazil, both the climatic niches of Lutzomyia longipalpis and VL cases were analysed. Distributional data were obtained from 55 of the 79 counties of MS between 2003-2012. Ecological niche models (ENM) of Lu. longipalpis and VL cases were produced using the maximum entropy algorithm based on eight climatic variables. Lu. longipalpis showed a wide distribution in MS. The highest climatic suitability for Lu. longipalpis was observed in southern MS. Temperature seasonality and annual mean precipitation were the variables that most influenced these models. Two areas of high climatic suitability for the occurrence of VL cases were predicted: one near Aquidauana and another encompassing several municipalities in the southeast region of MS. As expected, a large overlap between the models for Lu. longipalpis and VL cases was detected. Northern and northwestern areas of MS were suitable for the occurrence of cases, but did not show high climatic suitability for Lu. longipalpis . ENM of vectors and human cases provided a greater understanding of the geographic distribution of VL in MS, which can be applied to the development of future surveillance strategies. PMID:24402151

Predicting the geographic distribution of Lutzomyia longipalpis (Diptera: Psychodidae) and visceral leishmaniasis in the state of Mato Grosso do Sul, Brazil.

PubMed

Almeida, Paulo Silva de; Sciamarelli, Alan; Batista, Paulo Mira; Ferreira, Ademar Dimas; Nascimento, João; Raizer, Josué; Andrade Filho, José Dilermando; Gurgel-Gonçalves, Rodrigo

2013-12-01

To understand the geographic distribution of visceral leishmaniasis (VL) in the state of Mato Grosso do Sul (MS), Brazil, both the climatic niches of Lutzomyia longipalpis and VL cases were analysed. Distributional data were obtained from 55 of the 79 counties of MS between 2003-2012. Ecological niche models (ENM) of Lu. longipalpis and VL cases were produced using the maximum entropy algorithm based on eight climatic variables. Lu. longipalpis showed a wide distribution in MS. The highest climatic suitability for Lu. longipalpis was observed in southern MS. Temperature seasonality and annual mean precipitation were the variables that most influenced these models. Two areas of high climatic suitability for the occurrence of VL cases were predicted: one near Aquidauana and another encompassing several municipalities in the southeast region of MS. As expected, a large overlap between the models for Lu. longipalpis and VL cases was detected. Northern and northwestern areas of MS were suitable for the occurrence of cases, but did not show high climatic suitability for Lu. longipalpis. ENM of vectors and human cases provided a greater understanding of the geographic distribution of VL in MS, which can be applied to the development of future surveillance strategies.

Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil

PubMed Central

de Oliveira, Everton Falcão; Casaril, Aline Etelvina; Mateus, Nathália Lopes Fontoura; Murat, Paula Guerra; Fernandes, Wagner Souza; Oshiro, Elisa Teruya; de Oliveira, Alessandra Gutierrez; Galati, Eunice Aparecida Bianchi

2015-01-01

Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandflies. The specimens were caught in the city of Corumbá, state of Mato Grosso do Sul (Brazil) between October 2012-March 2014, and dissected to investigate flagellates and/or submitted to molecular analysis to detect Leishmania DNA. A total of 1,164 females (77.56% of which were Lutzomyia cruzi) representing 11 species were investigated using molecular analysis; 126 specimens of Lu. cruziwere dissected and also submitted to molecular analysis. The infection rate based on the presence of Leishmania DNA considering all the sandfly species analysed was 0.69%; only Leishmania (Leishmania) amazonensis was identified in Lu. cruzi by the molecular analysis. The dissections were negative for flagellates. This is the first record of the presence of L. (L.) amazonensis DNA in Lu. cruzi, and the first record of this parasite in this area. These findings point to the need for further investigation into the possible role of this sandfly as vector of this parasite. PMID:26602870

Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil.

PubMed

Oliveira, Everton Falcão de; Casaril, Aline Etelvina; Mateus, Nathália Lopes Fontoura; Murat, Paula Guerra; Fernandes, Wagner Souza; Oshiro, Elisa Teruya; Oliveira, Alessandra Gutierrez de; Galati, Eunice Aparecida Bianchi

2015-12-01

Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandflies. The specimens were caught in the city of Corumbá, state of Mato Grosso do Sul (Brazil) between October 2012-March 2014, and dissected to investigate flagellates and/or submitted to molecular analysis to detect Leishmania DNA. A total of 1,164 females (77.56% of which were Lutzomyia cruzi) representing 11 species were investigated using molecular analysis; 126 specimens of Lu. cruziwere dissected and also submitted to molecular analysis. The infection rate based on the presence of Leishmania DNA considering all the sandfly species analysed was 0.69%; only Leishmania (Leishmania) amazonensis was identified in Lu. cruzi by the molecular analysis. The dissections were negative for flagellates. This is the first record of the presence of L. (L.) amazonensis DNA in Lu. cruzi, and the first record of this parasite in this area. These findings point to the need for further investigation into the possible role of this sandfly as vector of this parasite.

Obesity and arterial hypertension in schoolchildren from Santa Cruz do Sul--RS, Brazil.

PubMed

Reuter, Éboni Marília; Reuter, Cézane Priscila; Burgos, Leandro Tibiriçá; Reckziegel, Miriam Beatris; Nedel, Fúlvio Borges; Albuquerque, Isabella Martins de; Pohl, Hildegard Hedwig; Burgos, Miria Suzana

2012-01-01

To verify the prevalence of obesity and hypertension in schoolchildren from Santa Cruz do Sul - RS, Brazil, in 2005 and 2008. The study was performed with two consecutive cross-sectional measurements, consisting of a stratified cluster sample, totaling 414 students, aged between 7 and 17 years, of which 215 (51.9%) were males and 199 (48.1%) were females. Obesity was assessed by body mass index (BMI) and percentage of body fat (%BF). Hypertension was measured by blood pressure values, both systolic (SBP) and diastolic (DBP). BMI assessment showed 18.6% and 22.3% of excess weight in males and 22.6% and 14.6% in females (in 2005 and 2008, respectively). Regarding obesity, the prevalence was 4.7% in both years for males and a reduction from 12.6% to 9.0% was observed in females. When analyzing the difference between assessments, there was significance in the BMI classification (p = 0.022) and %BF (p = 0.017) only in females. Statistically significant changes in SBP were found only in males (p < 0.001). The levels of excess weight, obesity, and %BF in females, as well as the increased levels of systolic blood pressure in males, demonstrate the need for early intervention through more effective public health campaigns.

Genetic divergence among invasive and native populations of the yellow peacock cichlid Cichla kelberi.

PubMed

Marques, A C P B; Franco, A C S; Salgueiro, F; García-Berthou, E; Santos, L N

2016-12-01

This study used the hypervariable domain of the mitochondrial DNA (mtDNA) control region (CR) to assess the genetic divergence among native and invasive populations of Cichla kelberi, which is considered the first peacock cichlid introduced and established throughout Brazil and is among the most invasive populations of this genus worldwide. The maximum likelihood tree based on 53 CR sequences with strong bootstrap support revealed that C. kelberi forms a monophyletic clade, confirming that all 30 C. kelberi studied belong to this morphotype. Additionally, the haplotype analysis of the C. kelberi sequences from 11 sampling sites revealed that invasive populations are much less diverse than native ones and largely dominated by a single haplotype that prevailed in reservoirs at the Paraíba do Sul River basin. Two haplotypes were recorded exclusively in an invasive population at Porto Rico, southern Brazil, and one private haplotype was detected in two reservoirs from Paraíba do Sul (Pereira Passos and Paracambi), suggesting more than one introduction event and that native populations should be better evaluated to encompass the entire genetic diversity of native C. kelberi. The possible route and pathways of C. kelberi introduction are also briefly discussed. © 2016 The Fisheries Society of the British Isles.

The sea-level highstand correlated to marine isotope stage (MIS) 7 in the coastal plain of the state of Rio Grande do Sul, Brazil.

PubMed

Lopes, Renato P; Dillenburg, Sergio R; Schultz, Cesar L; Ferigolo, Jorge; Ribeiro, Ana Maria; Pereira, Jamil C; Holanda, Elizete C; Pitana, Vanessa G; Kerber, Leonardo

2014-12-01

The coastal plain of the state of Rio Grande do Sul, in southern Brazil, includes four barrier-lagoon depositional systems formed by successive Quaternary sea-level highstands that were correlated to marine isotope stages (MIS) 11, 9, 5 and 1, despite the scarcity of absolute ages. This study describes a sea-level highstand older than MIS 5, based on the stratigraphy, ages and fossils of the shallow marine facies found in coastal barrier (Barrier II). This facies outcrops along the banks of Chuí Creek, it is composed of fine, well-sorted quartz sand and contains ichnofossils Ophiomorpha nodosa and Rosselia sp., and molluscan shells. The sedimentary record indicates coastal aggradation followed by sea-level fall and progradation of the coastline. Thermoluminescence (TL) and electron spin resonance (ESR) ages from sediments and fossil shells point to an age of ∼220 ka for the end of this marine transgression, thus correlating it to MIS 7 (substage 7e). Altimetric data point to a maximum amplitude of about 10 meters above present-day mean sea-level, but tectonic processes may be involved. Paleoceanographic conditions at the time of the highstand and correlations with other deposits in the Brazilian coasts are also discussed.

Antibiotic-resistant genes and antibiotic-resistant bacteria in the effluent of urban residential areas, hospitals, and a municipal wastewater treatment plant system.

PubMed

Li, Jianan; Cheng, Weixiao; Xu, Like; Strong, P J; Chen, Hong

2015-03-01

In this study, we determined the abundance of 8 antibiotics (3 tetracyclines, 4 sulfonamides, and 1 trimethoprim), 12 antibiotic-resistant genes (10 tet, 2 sul), 4 antibiotic-resistant bacteria (tetracycline, sulfamethoxazole, and combined resistance), and class 1 integron integrase gene (intI1) in the effluent of residential areas, hospitals, and municipal wastewater treatment plant (WWTP) systems. The concentrations of total/individual targets (antibiotics, genes, and bacteria) varied remarkably among different samples, but the hospital samples generally had a lower abundance than the residential area samples. The WWTP demonstrated removal efficiencies of 50.8% tetracyclines, 66.8% sulfonamides, 0.5 logs to 2.5 logs tet genes, and less than 1 log of sul and intI1 genes, as well as 0.5 log to 1 log removal for target bacteria. Except for the total tetracycline concentration and the proportion of tetracycline-resistant bacteria (R (2) = 0.330, P < 0.05), there was no significant correlation between antibiotics and the corresponding resistant bacteria (P > 0.05). In contrast, various relationships were identified between antibiotics and antibiotic resistance genes (P < 0.05). Tet (A) and tet (B) displayed noticeable relationships with both tetracycline and combined antibiotic-resistant bacteria (P < 0.01).

Prevalence of Antibiotic Resistance Genes and Bacterial Community Composition in a River Influenced by a Wastewater Treatment Plant

PubMed Central

Marti, Elisabet; Jofre, Juan; Balcazar, Jose Luis

2013-01-01

Antibiotic resistance represents a global health problem, requiring better understanding of the ecology of antibiotic resistance genes (ARGs), their selection and their spread in the environment. Antibiotics are constantly released to the environment through wastewater treatment plant (WWTP) effluents. We investigated, therefore, the effect of these discharges on the prevalence of ARGs and bacterial community composition in biofilm and sediment samples of a receiving river. We used culture-independent approaches such as quantitative PCR to determine the prevalence of eleven ARGs and 16S rRNA gene-based pyrosequencing to examine the composition of bacterial communities. Concentration of antibiotics in WWTP influent and effluent were also determined. ARGs such as qnrS, bla TEM, bla CTX-M, bla SHV, erm(B), sul(I), sul(II), tet(O) and tet(W) were detected in all biofilm and sediment samples analyzed. Moreover, we observed a significant increase in the relative abundance of ARGs in biofilm samples collected downstream of the WWTP discharge. We also found significant differences with respect to community structure and composition between upstream and downstream samples. Therefore, our results indicate that WWTP discharges may contribute to the spread of ARGs into the environment and may also impact on the bacterial communities of the receiving river. PMID:24205347

[Nutritional status of Kaingáng Indians enrolled in 12 indigenous schools in the State of Rio Grande do Sul, Brazil].

PubMed

Castro, Teresa Gontijo de; Schuch, Ilaine; Conde, Wolney Lisboa; Veiga, Juracilda; Leite, Maurício Soares; Dutra, Carmem Lucia Centeno; Zuchinali, Priccila; Barufaldi, Laura Augusta

2010-09-01

The study's objective was to characterize the nutritional status of 3,254 Kaingáng Indians in indigenous schools in Rio Grande do Sul State, Brazil. This was a school-based study. Weight (W), height (H), and waist circumference (WC) were measured according to World Health Organization guidelines (1995). Children's nutritional status classification included H/A, W/A, and W/H according to the National Center for Health Statistics (WHO, 1995) and H/A, W/A, and body mass index/age (BMI/A) according to WHO (2006). Adolescents were classified for BMI/A (WHO, 1995 and 2006) and H/A (WHO, 2006). Adults were classified for BMI (WHO, 1995) and WC (WHO, 2003). Adolescents represented 56% of the sample, children 42.5%, adults 1.4%, and elderly 0.1%. Prevalence rates for stunting were 15.1% (WHO, 1983) and 15.5% (WHO, 2006) in children and 19.9% in adolescents. Prevalence rates for overweight were 11% (WHO, 1983) and 5.7% (WHO, 2006) in children, 6.7% in adolescents, and 79.2% in adults. 45.3% of adults were at increased risk of metabolic diseases. A nutritional transition was observed in the group, characterized by significant prevalence of stunting in children and adolescents and prominent overweight in all age groups.

Prevalence of antibiotic resistance genes and bacterial pathogens in long-term manured greenhouse soils as revealed by metagenomic survey.

PubMed

Fang, Hua; Wang, Huifang; Cai, Lin; Yu, Yunlong

2015-01-20

Antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and HPB carrying ARGs pose a high risk to soil ecology and public health. Here, we used a metagenomic approach to investigate their diversity and abundance in chicken manures and greenhouse soils collected from Guli, Pulangke, and Hushu vegetable bases with different greenhouse planting years in Nanjing, Eastern China. There was a positive correlation between the levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in manures and greenhouse soils. In total, 156.2–5001.4 μg/kg of antibiotic residues, 22 classes of ARGs, 32 HPB species, and 46 species of HPB carrying ARGs were found. The highest relative abundance was tetracycline resistance genes (manures) and multidrug resistance genes (greenhouse soils). The dominant HPB and HPB carrying ARGs in the manures were Bacillus anthracis, Bordetella pertussis, and B. anthracis (sulfonamide resistance gene, sul1), respectively. The corresponding findings in greenhouse soils were Mycobacterium tuberculosis and M. ulcerans, M. tuberculosis (macrolide-lincosamide-streptogramin resistance protein, MLSRP), and B. anthracis (sul1), respectively. Our findings confirmed high levels of antibiotics, ARGs, HPB, and HPB carrying ARGs in the manured greenhouse soils compared with those in the field soils, and their relative abundance increased with the extension of greenhouse planting years.

Arsenic in groundwater of the Paraiba do Sul delta, Brazil: An atmospheric source?

PubMed

Mirlean, N; Baisch, P; Diniz, D

2014-06-01

High concentrations of arsenic (>50μg L(-1)) have been detected for the first time in groundwater of the wave-dominated Paraiba do Sul delta, Brazil. The deltaic shallow groundwater aquifer is enriched in arsenic fixed by authigenic sulfides. A study of palynomorphs confirmed that aquifer sediments were formed in inter-dune lakes/swamps lately covered by eolian sands. The organic sediments of contemporaneous inter-dune lake/swamp contain very high concentration of As: up to 180mg kg(-1) and 163μg L(-1) in dry gyttja material and interstitial water, respectively. The As in recent lake/swamp sediments is retained by iron hydroxides in upper and probably by sulfides in lower layers. In the absence of connection of inter-dune lakes/swamps with fluvial currents, the atmospheric input of As could be considered as the principal source in sediments. The calculation demonstrates the possibility of high concentrations of As accumulation in sediments of inter-dune lakes/swamps from atmospheric precipitations within several centuries before they will be covered by eolian sands and turned into shallow aquifer. Considering the commonalities of wave-dominated delta formations, we can predict more prevalent As accumulation in delta plain groundwater. Copyright © 2014 Elsevier B.V. All rights reserved.

Genetic analyses, phenotypic adaptability and stability in sugarcane genotypes for commercial cultivation in Pernambuco.

PubMed

Dutra Filho, J A; Junior, T C; Simões Neto, D E

2015-10-05

In the present study, we assessed the agro-industrial performance of 22 sugarcane genotypes adaptable to edaphoclimatic conditions in production microregions in the State of Pernambuco, Brazil, and we recommended the commercial cultivation of select genotypes. The variables analyzed were as follows: sucrose percentage in cane juice, tonnage of saccharose per hectare (TPH), sugarcane tonnage per hectare (TCH), fiber, solid soluble contents, total recoverable sugar tonnage (ATR), and total recoverable sugar tonnage per hectare (ATR t/ha). A randomized block design with 4 repeats was used. Combined variance of the experiments, genetic parameter estimates, and environment stratification were analyzed. Phenotypic adaptability and stability were analyzed using the Annicchiarico and Wricke methods and analysis of variance. Genetic gain was estimated using the classic index and sum of ranks. Genotype selection was efficient for TPH, TCH, and ATR t/ha. Genotypes presented a great potential for improvement and a similar response pattern in Litoral Norte and Mata Sul microregions for TPH and TCH and Litoral Norte and Litoral Sul microregions for ATR t/ha. Genotypes SP78-4764, RB813804, and SP79-101 showed better productivity and phenotypic adaptability and stability, according to the Wricke and Annicchiarico methods. These genotypes can be recommended for cultivation in the sugarcane belt in the State of Pernambuco.

Metazoan parasites of Mandi-amarelo Pimelodus maculatus and of Jundiá Rhamdia quelen (Osteichthyes: Siluriformes) of Paraíba do Sul River, Volta Redonda, Rio de Janeiro.

PubMed

Venancio, Aline Cristine Pinto; de Aguiar, Gesilene Ribeiro; Lopes, Patrícia da Silva; Alves, Dimitri Ramos

2010-01-01

Forty-one specimens of mandi-amarelo Pimelodus maculatus Lacépède, 1803 (Siluriformes: Pimelodidae) and 54 specimens of jundiá Rhamdia quelen (Quoy & Gaimard, 1824) (Siluriformes: Heptapteridae) were collected from the Paraíba do Sul River, Volta Redonda, State of Rio de Janeiro, Brazil between November 2007 and October 2008. These fish underwent necropsy so their infracommunities of metazoan parasites could be studied. The same three species of parasites were collected in the two fish species studied. These were one monogenean, one nematode, and one hirudinean. Cucullanus pinnai (Travassos, Artiga, and Pereira, 1928) (Nematoda: Cucullanidae) and Aphanoblastella sp. (Monogenea: Dactylogyridae) were the dominant species with the highest prevalence in P. maculatus and R. quelen. The parasite species of P. maculatus and R. quelen showed an atypical over-dispersed pattern of distribution. No parasite species showed significant correlation between the body total length of the siluriform hosts and their prevalence and abundance. The parasite species richness showed a mean value of 0.87 ± 0.67 (0-2) and 0.57 ± 0.56 (0-2) in P. maculatus and R. quelen, respectively, and no correlation with the body total length.

[Seasonal differences in falls and fractures among the elderly in the southern Brazilian state of Rio Grande do Sul].

PubMed

Caberlon, Iride Cristofoli; Bós, Ângelo José Gonçalves

2015-12-01

Falls and fractures in the elderly represent a significant public health problem, associated with higher rates of morbidity and mortality, reduction of functional capacity, institutionalization of the elderly and early death. The scope of this study was to investigate falls and fractures in the elderly living in the metropolitan and mountainous region of the state of Rio Grande do Sul, analyzing the associated factors, seasonality and severity thereof. It is a cross-sectional, retrospective, descriptive, analytical and quantitative study of the elderly attended for falls from January 1 to December 31 of 2010 using secondary data from emergency units of the Brazilian Unified Health System. Data were collected from all health care reports filed in medical departments. The total number of bulletins for the elderly attended for falls was 6,556: 71% were women; 26.8% were in winter; 30% involved fractures (32% women and 28% men) (p < 0.0001). In only 17.2% of the cases the fall site was recorded, and 58% were outside the home. Winter was the season with 34% of confirmed fractures (p = 0.0002) with more severe outcomes (26.3%). Most falls and their consequences can be prevented and avoided. It is paramount to implement programs and multifactorial actions for intervention.

Aids mortality trends according to sociodemographic characteristics in Rio Grande do Sul State and Porto Alegre City, Brazil: 2000-2011.

PubMed

Cunha, Ana Paula da; Cruz, Marly Marques da; Torres, Raquel Maria Cardoso

2016-01-01

to analyze AIDS mortality trends in Rio Grande do Sul State (RS) and Porto Alegre City (POA) according to sociodemographic characteristics in the period 2000-2011. this was an ecological time series study of AIDS mortality rates; Prais-Winsten regression model was used. the standardized mortality AIDS rate showed a stationary trend in RS (1.3%; 95%CI: -0..;6.7) and in POA (-0.3%; 95%CI: -5.1;3.9); there was an increasing trend in the following categories: women in RS (4.1%; 95%CI: 3.0; 5.3) and in POA (2.7%; 95%CI: 1.8; 3.5), people with brown skin color in RS (4.5%; 95%CI: 1.9; 7.2) and in POA (4.6%; 95%CI: 1.5; 7.9), the 40-49 age group in RS (4.0%; 95%CI: 1.3; 6.7), the 50-59 age group in RS (5.8%; 95%CI: 1.9; 9.9) and in POA (6.0%; 95%CI: 2.1; 9.9), and the >60 age group in RS (4.0%; 95%CI: 1.1; 6.9). AIDS mortality increased among women, individuals with brown skin color and older age groups in RS and POA.

Immunological Profile of the Yellow Clam Mesodesma mactroides (Mesodesmatidae) from the Southern Coast of Rio Grande do Sul, Brazil.

PubMed

Silva Santos, Juan Jethro; Carvalho, Yuri Bovi; de Alcantara Lopes, Diogo Luiz; Romano, Luis Alberto

2016-03-01

The yellow clam Mesodesma mactroides (Mesodesmatidae) is a sandy beach bivalve that is distributed from Rio de Janeiro, Brazil, to the south of Buenos Aires province, Argentina. The yellow clam population has been declining in recent decades. To increase our understanding of this species, we evaluated the immunological status of yellow clams collected during different seasons from various areas in the state of Rio Grande do Sul, Brazil. We characterized the hemocytes, determined the differential hemocyte counts (DHCs), calculated the apoptotic index, and evaluated the incidence of parasites in yellow clams through histological analysis. We identified two types of hemocyte (hyaline and granular) that showed significant variation in DHCs among sampling areas during the summer and winter. The apoptotic index only exhibited significant variation during the summer. Histopathological analysis results did not significantly differ among sampling areas. This work demonstrated that environmental variation (e.g., temperature and salinity) associated with anthropogenic actions may be affecting the immune system of yellow clams. However, more studies are needed to determine the full influence of these factors on the yellow clam's immune system and thus contribute to future management and aquaculture of the species. Received May 10, 2015; accepted October 28, 2015.

Identification and antimicrobial suceptibility profile of bacteria causing bovine mastitis from dairy farms in Pelotas, Rio Grande do Sul.

PubMed

Freitas, C H; Mendes, J F; Villarreal, P V; Santos, P R; Gonçalves, C L; Gonzales, H L; Nascente, P S

2018-01-08

Mastitis is an inflammatory process of the udder tissue caused mainly by the bacteria Staphylococcus aureus. The indiscriminate use of antibiotics fosters conditions that favor the selection of resistant microorganisms, suppressing at the same time susceptible forms, causing a serious problem in dairy cattle. Given the importance in performing an antibiogram to select the most adequate antimicrobial therapy, the aim of this study was to identify bacteria isolated from cow's milk with mastitis, in dairy farms situated in the city of Pelotas, Rio Grande do Sul, and to determinate the susceptibility profile of these isolates against the antibiotics used to treat this illness. A total of 30 isolates of Staphylococcus spp., were selected from milk samples from the udder quarters with subclinical mastitis whose species were identified through the Vitek system. The susceptibility profile was performed by the disk diffusion assay, against: ampicillin, amoxicillin, bacitracin, cephalexin, ceftiofur, enrofloxacin, gentamicin, neomycin, norfloxacin, penicillin G, tetracycline and trimethoprim. In the antibiogram, 100.0% of the isolates were resistant to trimethoprim and 96.7% to tetracycline and neomycin, three strains of Staphylococcus spp., (10.0%) presented resistance to the 12 antibiotics tested and 24 (80.0%) to at least eight. These results showed the difficulty in treating mastitis, due to the pathogens' resistance.

The sea-level highstand correlated to marine isotope stage (MIS) 7 in the coastal plain of the state of Rio Grande do Sul, Brazil.

PubMed

Lopes, Renato P; Dillenburg, Sergio R; Schultz, Cesar L; Ferigolo, Jorge; Ribeiro, Ana Maria; Pereira, Jamil C; Holanda, Elizete C; Pitana, Vanessa G; Kerber, Leonardo

2014-12-09

The coastal plain of the state of Rio Grande do Sul, in southern Brazil, includes four barrier-lagoon depositional systems formed by successive Quaternary sea-level highstands that were correlated to marine isotope stages (MIS) 11, 9, 5 and 1, despite the scarcity of absolute ages. This study describes a sea-level highstand older than MIS 5, based on the stratigraphy, ages and fossils of the shallow marine facies found in coastal barrier (Barrier II). This facies outcrops along the banks of Chuí Creek, it is composed of fine, well-sorted quartz sand and contains ichnofossils Ophiomorpha nodosa and Rosselia sp., and molluscan shells. The sedimentary record indicates coastal aggradation followed by sea-level fall and progradation of the coastline. Thermoluminescence (TL) and electron spin resonance (ESR) ages from sediments and fossil shells point to an age of ∼220 ka for the end of this marine transgression, thus correlating it to MIS 7 (substage 7e). Altimetric data point to a maximum amplitude of about 10 meters above present-day mean sea-level, but tectonic processes may be involved. Paleoceanographic conditions at the time of the highstand and correlations with other deposits in the Brazilian coasts are also discussed.

Genetic progress in homogeneous regions of wheat cultivation in Rio Grande do Sul State, Brazil.

PubMed

Follmann, D N; Cargnelutti Filho, A; Lúcio, A D; de Souza, V Q; Caraffa, M; Wartha, C A

2017-03-30

The State of Rio Grande do Sul (RS) stands out as the largest wheat producer in Brazil. Wheat is the most emphasized winter cereal in RS, attracting public and private investments directed to wheat genetic breeding. The study of genetic progress should be performed routinely at breeding programs to study the behavior of cultivars developed for homogeneous regions of cultivation. The objectives of this study were: 1) to evaluate the genetic progress of wheat grain yield in RS; 2) to evaluate the influence of cultivar competition trial stratification in homogeneous regions of cultivation on the study of genetic progress. Grain yield data of 122 wheat cultivars evaluated in 137 trials arranged in randomized block design with three or four replications were used. Field trials were carried out in 23 locations in RS divided into two homogeneous regions during the period from 2002 to 2013. Genetic progress for RS and homogeneous regions was studied utilizing the method proposed by Vencovsky. Annual genetic progress for wheat grain yield during the period of 12 years in the State of RS was 2.86%, oscillating between homogeneous regions of cultivation. The difference of annual genetic progress in region 1 (1.82%) in relation to region 2 (4.38%) justifies the study of genetic progress by homogeneous regions of cultivation.

Ecological aspects of phlebotomines (Diptera: Psychodidae) in endemic area of visceral leishmaniasis, Campo Grande, State of Mato Grosso do Sul, Brazil.

PubMed

Oliveira, Alessandra Gutierrez; Galati, Eunice Aparecida Bianchi; Fernandes, Carlos Eurico; Dorval, Maria Elizabeth Cavalheiros; Brazil, Reginaldo Peçanha

2012-01-01

Aspects of phlebotomine behavior were investigated in the city of Campo Grande, Mato Grosso do Sul state. The insects were captured weekly during December 2003 to November 2005, with Centers for Disease Control light traps at seven different sites including forests and residential areas. In total, 11,024 specimens (7,805 males and 3,219 females) were collected, from which 9,963 (90.38%) were identified as Lutzomyia longipalpis, the proven vector of American visceral leishmaniasis agent. The remaining 9.62% comprised 21 species. L. longipalpis was the most frequent species in all sampled sites, and the first in the ranking of standardized species abundance index. In residential areas this species clearly predominated in the peridomicile (90.96%), in contrast to the intradomicile (9.04%); in animal shelters, it was more numerous in hen houses and prevailed at ground level, inside, and at forest edge around the residences; this aspect is worrying because this insect may remain sheltered in forested environments during the use of insecticides in homes. In the forest environment, other probable or proven vector of cutaneous leishmaniasis agents were also captured such as Lutzomyia whitmani (=Nyssomyia whitmani, sensu Galati), Lutzomyia antunesi (=Nyssomyia antunesi, sensu Galati), and Lutzomyia flaviscutellata (=Bichromomyia flaviscutellata, sensu Galati).

Vulnerability Assessment of a Coastal Dune System at São Francisco do Sul Island, Santa Catarina, Brazil

NASA Astrophysics Data System (ADS)

Alquini, Fernanda; Bertoni, Duccio; Sarti, Giovanni; Ciccarelli, Daniela; Pozzebon, Alessandro; Ferreira de Melo Júnior, João Carlos; Voos Vieira, Celso

2016-10-01

In this paper a Coastal Dune Vulnerability Index (CDVI) has been applied on a beach located in the eastern side of Sao Francisco do Sul Island (Brazil). The aim of this study is to assess the vulnerability of a coastal dune system and to identify the areas that result most sensitive to environmental changes. The CDVI has been applied along six transects traced out on two sectors that have been selected based on dune characteristics: Zone A is characterized by well developed parabolic dunes, whereas Zone B is characterized by transverse dunes. The analysis involved 51 quantitative and qualitative variables, divided into five groups: geomorphological dune system condition, marine influence, Aeolian effect, vegetation condition and human effect. The total CDVI was computed as the unweighted average of the partial vulnerability indices. In summary, the total vulnerability can be classified as medium: the geomorphological factor must be monitored at Grande beach, in particular the blowouts in Zone A and the frontal dune retreat in Zone B. The results of the study confirm that the management of coastal areas might be improved using a tool such as the CDVI, which can be easily applied on a regular basis to take under control the factors that mostly affect the evolution of the site.

[Environmental and biological determinants of neuropsychomotor development in a sample of children in Canoas/RS].

PubMed

Pilz, Elsa Maria Luz; Schermann, Lígia Braun

2007-01-01

The object of this study is to determine the prevalence of potential delays in neuropsychomotor development and their possible association with, on one hand, environmental and biological factors, and maternal competence on the other, in a sample of children up to six years old living in Canoas, in Rio Grande do Sul state. A questionnaire was submitted to mothers including questions on social, economic and reproduction factors; child's conditions at birth; child's pathologies; family structure; child care and elements on maternal competence. The potential for neuropsychomotor development delay was assessed by the Denver II Test. Forty clusters were visited in Canoas, a city in Rio Grande do Sul state, in accordance with the cluster probabilistic sampling process. From 197 children assessed by this analytical cross-section study, there was a 27% (n=53) prevalence of potential delay in neuropsychomotor development. The multivariate analysis showed that factors associated with potential development delays were: low income (or = 9,3); mothers with less than 18-month intervals between pregnancies (or=3,9) ; and lack of support from child's father (or=7,0). These results support the importance of implementing income generating programs, health education, and family planning in order to prevent child development delays.

Intestinal parasitism among waste pickers in Mato Grosso do Sul, Midwest Brazil

PubMed Central

Higa, Minoru German; Cardoso, Wesley Márcio; Weis, Sabrina Moreira dos Santos; França, Adriana de Oliveira; Pontes, Elenir Rose Jardim Cury; da Silva, Patrícia Vieira; de Oliveira, Márcia Pereira; Dorval, Maria Elizabeth Moraes Cavalheiros

2017-01-01

ABSTRACT The purpose of this study was to estimate the prevalence of intestinal parasites in both cooperative-affiliated and independent waste pickers operating at the municipal sanitary landfill in Campo Grande, Mato Grosso do Sul, Brazil, and associate these findings with hemoglobin, eosinophils, vitamin A and C levels and interleukin 5 and 10 (IL-5 and IL-10) production. Biological samples were collected, in addition to clinical, epidemiological, and sociodemographic data. Stool analyzes were based on sedimentation by centrifugation and on spontaneous sedimentation. High-performance liquid chromatography was used to determine vitamin A and C levels. ELISA was employed to quantify interleukins. Intestinal parasites were found in 29 of the 66 subjects assessed (43.9%). Endolimax nana (22.7%), Entamoeba coli (21.1%), Giardia lamblia (6.1%), Entamoeba histolytica/E. dispar (4.5%), and Ascaris lumbricoides (4.5%) were the most prevalent species. Pathogenic parasites were detected in 11 individuals (16.7%). Hypovitaminoses A and C were detected in 19.6% (13/66) and 98.4% (65/66) of subjects, respectively. IL-5 and IL-10 production was observed in 21 (31.8%) and 32 (48.4%) subjects, respectively. Infection with pathogenic intestinal parasites was not a cause of vitamin A and C deficiency or IL-5 and IL-10 production among these workers. PMID:29267595

Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water.

PubMed

Shi, Peng; Jia, Shuyu; Zhang, Xu-Xiang; Zhang, Tong; Cheng, Shupei; Li, Aimin

2013-01-01

This study aimed to investigate the chlorination effects on microbial antibiotic resistance in a drinking water treatment plant. Biochemical identification, 16S rRNA gene cloning and metagenomic analysis consistently indicated that Proteobacteria were the main antibiotic resistant bacteria (ARB) dominating in the drinking water and chlorine disinfection greatly affected microbial community structure. After chlorination, higher proportion of the surviving bacteria was resistant to chloramphenicol, trimethoprim and cephalothin. Quantitative real-time PCRs revealed that sulI had the highest abundance among the antibiotic resistance genes (ARGs) detected in the drinking water, followed by tetA and tetG. Chlorination caused enrichment of ampC, aphA2, bla(TEM-1), tetA, tetG, ermA and ermB, but sulI was considerably removed (p < 0.05). Metagenomic analysis confirmed that drinking water chlorination could concentrate various ARGs, as well as of plasmids, insertion sequences and integrons involved in horizontal transfer of the ARGs. Water pipeline transportation tended to reduce the abundance of most ARGs, but various ARB and ARGs were still present in the tap water, which deserves more public health concerns. The results highlighted prevalence of ARB and ARGs in chlorinated drinking water and this study might be technologically useful for detecting the ARGs in water environments. Copyright © 2012 Elsevier Ltd. All rights reserved.

Synthesis of benthic flux components in the Patos Lagoon coastal zone, Rio Grande do Sul, Brazil

NASA Astrophysics Data System (ADS)

King, J. N.

2012-12-01

The primary objective of this work is to synthesize components of benthic flux in the Patos Lagoon coastal zone, Rio Grande do Sul, Brazil. Specifically, the component of benthic discharge flux forced by the terrestrial hydraulic gradient is 0.8 m3 d-1; components of benthic discharge and recharge flux associated with the groundwater tidal prism are both 2.1 m3 d-1; components of benthic discharge and recharge flux forced by surface-gravity wave setup are both 6.3 m3 d-1; the component of benthic discharge flux that transports radium-228 is 350 m3 d-1; and components of benthic discharge and recharge flux forced by surface-gravity waves propagating over a porous medium are both 1400 m3 d-1. (All models are normalized per meter shoreline.) Benthic flux is a function of components forced by individual mechanisms and nonlinear interactions that exist between components. Constructive and destructive interference may enhance or diminish the contribution of benthic flux components. It may not be possible to model benthic flux by summing component magnitudes. Geochemical tracer techniques may not accurately model benthic discharge flux or submarine groundwater discharge (SGD). A conceptual model provides a framework on which to quantitatively characterize benthic discharge flux and SGD with a multifaceted approach.

Occurrence and distribution of antibiotic resistance genes in water supply reservoirs in Jingjinji area, China.

PubMed

Zhang, Kai; Niu, Zhi-Guang; Lv, Zhiwei; Zhang, Ying

2017-11-01

Jingjinji area occupies important position in developing of the Chinese economy, while there exists a sharp conflict between economic growth and limited water resources in this area. To ensure the safety of water consumption of cities in Jingjinji area, we investigated the abundance of three classes of antibiotic resistance genes (ARGs) in water and sediment of six water supply reservoirs in this area. The results showed that the detection frequency of sul1, tetM and ermB were 100%. However, the content ranges of these genes were different (10 -5 to 10 -2 /16S gene copies for sul1, 10 -5 to 10 -3 /16S gene copies for ermB, and 10 -5 to 10 -3 /16S gene copies for tetM). The content of ribosome protection proteins (RPP) genes were the highest in all selected tet genes. The highest abundance of ARGs in water and sediment samples was sampled from Panjiakou reservoir and Guanting reservoir, respectively. Except COD, chla and tetM, there are no significant correlation between water quality parameters and ARGs. Overall, this study provides integrated profiles of the three types of ARGs in water supply reservoirs of Jingjinji area and thus helps to re-evaluate the effects of human activities to water supply reservoirs.

Synthesis of benthic flux components in the Patos Lagooncoastal zone, Rio Grande do Sul, Brazil

USGS Publications Warehouse

King, Jeffrey N.

2012-01-01

The primary objective of this work is to synthesize components of benthic flux in the Patos Lagoon coastal zone, Rio Grande do Sul, Brazil. Specifically, the component of benthic discharge flux forced by the terrestrial hydraulic gradient is 0.8 m3 d-1; components of benthic discharge and recharge flux associated with the groundwater tidal prism are both 2.1 m3 d-1; components of benthic discharge and recharge flux forced by surface-gravity wave setup are both 6.3 m3 d-1; the component of benthic discharge flux that transports radium-228 is 350 m3 d-1; and components of benthic discharge and recharge flux forced by surface-gravity waves propagating over a porous medium are both 1400 m3 d-1. (All models are normalized per meter shoreline.) Benthic flux is a function of components forced by individual mechanisms and nonlinear interactions that exist between components. Constructive and destructive interference may enhance or diminish the contribution of benthic flux components. It may not be possible to model benthic flux by summing component magnitudes. Geochemical tracer techniques may not accurately model benthic discharge flux or submarine groundwater discharge (SGD). A conceptual model provides a framework on which to quantitatively characterize benthic discharge flux and SGD with a multifaceted approach.

The influence of industrial and agricultural waste on water quality in the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil).

PubMed

da Rocha, Monyque Palagano; Dourado, Priscila Leocadia Rosa; de Souza Rodrigues, Mayara; Raposo, Jorge Luiz; Grisolia, Alexeia Barufatti; de Oliveira, Kelly Mari Pires

2015-07-01

Water quality monitoring is used to determine the impact of human activities on the environment. We evaluated water quality in the Água Boa stream, located within the municipality of Dourados, State of Mato Grosso do Sul, Brazil, by analyzing physico-chemical, chemical, and microbiological parameters, as well as chlorophyll concentrations. Five sets of water samples were collected between December 2012 and November 2013 from three locations within the stream. The results showed the presence of Escherichia coli and antibiotic-resistant Pseudomonas spp. strains and high concentrations of organic matter (total dissolved solids), inorganic species (Mg, Ca, and Fe), and agrochemical residues (thiamethoxam). The main stream water contaminants are derived from urban, industrial, and agricultural activities within the watershed. Given the presence of contaminants, it is important that such findings are disseminated in order to highlight the risks that contact with this water may pose to human health. To preserve the environment and improve site conditions, people would need to participate by demanding that normative national and international standards be respected and that the situation be supervised by the competent governmental agencies; this would make it possible to reverse or minimize contamination problems within the Água Boa stream.

Mitochondrial genetic variability of Didelphis albiventris (Didelphimorphia, Didelphidae) in Brazilian localities

PubMed Central

Sousa, Luciene C.C.; Gontijo, Célia M.F.; Botelho, Helbert A.; Fonseca, Cleusa G.

2012-01-01

Didelphis albiventris is a well-known and common marsupial. Due to its high adaptability, this very widespread generalist species occurs under various environmental conditions, this even including protected regions and disturbed urban areas. We studied a 653 bp fragment of cytochrome oxidase c (COI) from 93 biological samples from seven Brazilian localities, with linear distances ranging between 58 and about 1800 km to analyze the effects of geographic distances on variability and genetic differentiation. The haplotype network presented nine haplotypes and two genetic clusters compatible with the two most distant geographic areas of the states of Minas Gerais, in the southeast, and Rio Grande do Sul, in the extreme south. As each cluster was characterized by low nucleotide and high haplotype diversities, their populations were obviously composed of closely related haplotypes. Surprisingly, moderate to high FST differentiation values and a very weak phylogeographic signal characterizes interpopulation comparisons within Minas Gerais interdemes, these being correlated with the presence of privative haplotypes. On a large rgeographic scale, a comparison between demes from Minas Gerais and Rio Grande do Sul presented high FST values and a robust phylogeographic pattern. This unexpected scenario implies that mtDNA gene flow was insufficient to maintain population cohesion, reflected by the observed high differentiation. PMID:22888303

Holocene history of a lake filling and vegetation dynamics of the Serra Sul dos Carajás, southeast Amazonia.

PubMed

Guimarães, José T F; Sahoo, Prafulla K; Souza-Filho, Pedro W M; Figueiredo, Mariana M J Costa DE; Reis, Luiza S; Silva, Marcio S DA; Rodrigues, Tarcísio M

2017-07-24

Down-core changes in sedimentary facies, elemental geochemistry, pollen, spore, δ13C, δ15N and radiocarbon records from a filled lake, named R4, of the Serra Sul dos Carajás were used to study the relationship between the paleomorphological and paleoecological processes and their significance for Holocene paleoclimatology of the southeast Amazonia. The sediment deposition of the R4 lake started around 9500 cal yr BP. Increase of detrital components from 9500 to 7000 cal yr BP suggests high weathering of surrounding catchment rocks and soils, and deposition into the lake basin under mudflows. At that time, montane savanna and forest formation were already established suggesting predominance of wet climate. However, from 7000 to 3000 cal yr BP, a decline of detrital input occurred. Also, forest formation and pteridophytes were declined, while palms and macrophytes were remained relatively stable, indicating that water levels of the lake is likely dropped allowing the development of plants adapted to subaerial condition under drier climate conditions. After 3000 cal yr BP, eutrophication and low accommodation space lead to high lake productivity and the final stage of the lake filling respectively, and forest formation may has acquired its current structure, which suggests return of wetter climate conditions.

Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues.

PubMed

Ye, Mao; Sun, Mingming; Feng, Yanfang; Wan, Jinzhong; Xie, Shanni; Tian, Da; Zhao, Yu; Wu, Jun; Hu, Feng; Li, Huixin; Jiang, Xin

2016-05-15

Considering the potential threat of vegetables growing in antibiotic-polluted soil with high abundance of antibiotic-resistant genes (ARGs) against human health through the food chain, it is thus urgent to develop novel control technology to ensure vegetable safety. In the present work, pot experiments were conducted in lettuce cultivation to assess the impedance effect of biochar amendment on soil sulfonamides (SAs), antibiotic-resistant bacteria (ARB), and ARG enrichment in lettuce tissues. After 100 days of cultivation, lettuce cultivation with biochar amendment exhibited the greatest soil SA dissipation as well as the significant improvement of lettuce growth indices, with residual soil SAs mainly existing as the tightly bound fraction. Moreover, the SA contents in roots and new/old leaves were reduced by one to two orders of magnitude compared to those without biochar amendment. In addition, isolate counts for SA-resistant bacterial endophytes in old leaves and sul gene abundances in roots and old leaves also decreased significantly after biochar application. However, neither SA resistant bacteria nor sul genes were detected in new leaves. It was the first study to demonstrate that biochar amendment can be a practical strategy to protect lettuce safety growing in SA-polluted soil with rich ARB and ARGs. Copyright © 2015 Elsevier B.V. All rights reserved.

Biodiversity and spatial distribution of epiphytic ferns on Alsophila setosa Kaulf. (Cyatheaceae) caudices in Rio Grande do Sul, Brazil.

PubMed

Schmitt, J L; Windisch, P G

2010-08-01

The extractive exploitation of the tree fern Alsophila setosa Kaulf. alters forest formations and diminishes the availability of micro-habitat for epiphytes. A survey of epiphytic fern communities on A. setosa at 16 study sites in different forest formations in the State of Rio Grande do Sul, Brazil, documented the occurrence of 31 species representing 16 genera and six families. The greatest richness of species occurred in Polypodiaceae (39%) and in the genus Asplenium L. (22%). Habitual holoepiphyte was the predominant ecological category, representing 61% of the species. Similarity analysis demonstrated heterogeneity in the composition of the epiphytic ferns at the study sites and indicated that the vegetation type is not the main factor for floristic difference. The lowest total specific richness (5) was recorded for the seasonal deciduous forest site. The majority of the sites presented similar averages of phorophyte height and epiphyte richness per caudex. In 25% of the sites the height of the host plants presented significant correlation with specific richness. Considering that the majority of the epiphytes are habitual and that some of them occur exclusively or preferentially on tree ferns, the maintenance of these host plants in the vegetation is essential for the conservation of epiphytic species.

Microcomputer Network for Computerized Adaptive Testing (CAT)

DTIC Science & Technology

1984-03-01

PRDC TR 84-33 \\Q.�d-33- \\ MICROCOMPUTER NETWOJlt FOR COMPUTERIZED ADAPTIVE TESTING ( CAT ) Baldwin Quan Thomas A . Park Gary Sandahl John H...ACCEIIION NO NPRDC TR 84-33 4. TITLE (-d Sul>tlllo) MICROCOMP UTER NETWORK FOR COMPUTERIZED ADA PTIVE TESTING ( CAT ) 1. Q B. uan T. A . Park...adaptive testing ( CAT ) Bayesian sequential testing 20. ABSTitACT (Continuo on ro•••• aide II noco .. _, _., ld-tlly ,.,. t.loclt _._.) DO Computerized

Bibliography on Future Trends in Terrorism

DTIC Science & Technology

1998-09-01

Bernhard Restel, et alia. "La police en interfaces: Colloque universitaire de l’Institut Suisse de Police," Revue Internationale de Criminologie et...Convenzione di Ginevra per l’Istituzione di una Corte Penale Internazionale sul Terrorismo : Un document da tornare a leggere," Il Politico, [Rome], 62...The author, who is president of Peru , points out that terrorist organizations in Peru have struggled for a decade to erode the democratic system and

New species and records of cunaxid mites (Acari: Cunaxidae) from soil in Southern Brazil.

PubMed

Rocha, Matheus Dos Santos; Rodrigues, Everton Nei Lopes; Ferla, Noeli Juarez

2015-07-02

Neocunaxoides promatae sp. nov., Bonzia flechtmanni sp. nov. and Dactyloscirus multiscutus sp. nov. are described from soil and leaf litter in Atlantic rainforest and Atlantic Araucaria forest in natural environments in São Francisco de Paula municipality, Rio Grande do Sul State, Brazil. In addition, the species Pulaeus quadrisolenidius Castro & Den Heyer 2009 and Lupaeus lectus Castro & Den Heyer (2009) are registered for the first time in this State.

Impact of Alloying on Microbiologically Influenced Corrosion. A Review

DTIC Science & Technology

1993-09-01

H.A. Videla, Acta MIC. Chromium and molybdenum sion 47,9 (1991): p. 674. Cientifica Venezolana 35 (1984): p. 244. additions as high as 20 and 6%, re...conductivity, described a model that predicts sul- logical settlement. Biofilms produce ease of soldering and brazing , and fide-induced corrosion in the pres...highly aerated, the area immediately under the de- (Cu3,S16) formation hasbeen reported high -velocity seawater applications. posit becomes a

Real-Time Prediction of Tropical Cyclone Intensity Using COAMPS-TC

DTIC Science & Technology

2012-01-01

tropospheric (UT) cloud fields (i.e., cirrus clouds) long after the initial eruption cycle from gradual particle settling and re-entrainment back into the... troposphere . Volcanic sul- fur dioxide and hydrogen sulfide vapor molecules are photo-oxidized in the LS, forming gaseous sulphuric acid, which in...concentration over the eastern United States at 1815 UTC on the 17th shown in Fig. 5(a), derived from NASA Ozone Monitoring Instrument (OMI) measurements

Latin America Report, No. 2756

DTIC Science & Technology

1983-10-25

144; BRAZIL CHILE Bigger Harvests Expected in Parana, Rio Grande do Sul (0 ESTADO DE SAO PAULO, various dates) 14 Parana Production Rio...to equipourNavy adequately." 6362 CSOj 3342/6 COUNTRY SECTION CHILE CABINET SHAKEUP, POLITICAL SITUATION ANALYZED Jarpa’s Mission Santiago...ERCILLA in Spanish 17 Aug 83 pp 8-9 /Xrticle by Carmen Gardewi£7 /Text/ In only 1 week, Chile has become a different country. The rapidity with which

U. S. (United States) Air Force Fuel Cell Application Analysis.

DTIC Science & Technology

1982-01-01

Desulfurizer and shift cata- lyst temperatures are maintained by controlling the amount of gas entering or by-passing the external water vaporizer. If...rich gas . The sul- fur content of the desulfurized fuel gas must be less than 1 ppm. Reforming takes place in a nickel catalyst bed, operating at... Control Supplemental Firing Fuel Cell Temperature Recirculation Air Temperature Control via Cooler Fan Speed Exhaust Gas Water Load Following damper

Brazil wheat yield covariance model

NASA Technical Reports Server (NTRS)

Callis, S. L.; Sakamoto, C.

1984-01-01

A model based on multiple regression was developed to estimate wheat yields for the wheat growing states of Rio Grande do Sul, Parana, and Santa Catarina in Brazil. The meteorological data of these three states were pooled and the years 1972 to 1979 were used to develop the model since there was no technological trend in the yields during these years. Predictor variables were derived from monthly total precipitation, average monthly mean temperature, and average monthly maximum temperature.

Translationa on Environmental Quality No. 184.

DTIC Science & Technology

1978-11-06

sonnel and facilities for quak« defection . The team sent by the Minis- try of Science and Technology also urged the government to conduct a nation...Municipal Department of Water and Sewers, Napoleao Rodrigues de Freitas shows why. Pesticides DMAE [Municipal Department of Water and Sewers] report...do Sul region," the report states, "causes great concern for the DMAE because it serves as a supply 15 source for the city of Porto Alegre. The

Characterization of a Mud Deposit Offshore of the Patos Lagoon, Southern Brazil

DTIC Science & Technology

2009-02-01

Journal Article 3. DATES COVERED (From - To) 4. TITLE AND SUBTITLE Characterization of mud deposit offshore of the Patos lagoon, southern Brazil 5a...deposition of mud on the beach along the shoreface of Rio Grande do Sul, Brazil dramatically influences the normal operations in the littoral zone...Continental Shelf Research journal homepage: www.elsevier.com/locate/csr Characterization of a mud deposit offshore of the Patos Lagoon, southern Brazil

HOSPITALIZATIONS FOR CHOLECYSTITIS AND CHOLELITHIASIS IN THE STATE OF RIO GRANDE DO SUL, BRAZIL.

PubMed

Nunes, Emeline Caldana; Rosa, Roger Dos Santos; Bordin, Ronaldo

2016-01-01

The cholelithiasis is disease of surgical resolution with about 60,000 hospitalizations per year in the Sistema Único de Saúde (SUS - Brazilian National Health System) of the Rio Grande do Sul state. To describe the profile of hospitalizations for cholecystitis and cholelithiasis performed by the SUS of Rio Grande do Sul state, 2011-2013. Hospital Information System data from the National Health System through morbidity list for cholelithiasis and cholecystitis (ICD-10 K80-K81). Variables studied were sex, age, number of hospitalizations and approved Hospitalization Authorizations (AIH), total amount and value of hospital services generated, days and average length of stay, mortality, mortality and case fatality ratio, from health regions of the Rio Grande do Sul. During 2011-2013 there were 60,517 hospitalizations for cholecystitis and cholelithiasis, representing 18.86 hospitalizations per 10,000 inhabitants/year, most often in the age group from 60 to 69 years (41.34 admissions per 10,000 inhabitants/year) and female (27.72 hospitalizations per 10,000 inhabitants/year). The fatality rate presented an inverse characteristic: 13.52 deaths per 1,000 admissions/year for males, compared with 7.12 deaths per 1,000 admissions/year in females. The state had an average total amount spent and value of hospital services of R$ 16,244,050.60 and R$ 10,890,461.31, respectively. The health region "Capital/Gravataí Valley" exhibit the highest total expenditure and hospital services, and the largest number of deaths, and average length of stay. The hospitalization and lethality coefficients, the deaths, the length of stay and spending related to admissions increased from 50 years old. Females had a higher frequency and higher values ​​spent on hospitalization, while the male higher coefficient of mortality and mean hospital stay. A colelitíase é doença de resolução cirúrgica com cerca de 60.000 internações por ano no Sistema Único de Saúde no estado do Rio Grande do Sul. Descrever o perfil das internações por colecistite e colelitíase na rede pública do estado no triênio 2011-2013. Emprego de dados do Sistema de Informações Hospitalares do Sistema Único de Saúde, através da lista de morbidades para colelitíase e colecistite (CID-10 K80-K81). As variáveis estudadas foram: sexo, idade, número de internações e de Autorizações de Internação Hospitalar aprovadas, valor total e valor dos serviços hospitalares gerados, dias e média de permanência, óbitos, coeficiente de mortalidade e letalidade, a partir das regiões de saúde do estado. No triênio ocorreram 60.517 internações por colecistite e colelitíase, o que representou 18,86 internações por 10.000 habitantes/ano, mais frequente na faixa etária dos 60 aos 69 anos (41,34 internações por 10.000 habitantes/ano) e no sexo feminino (27,72 hospitalizações por 10.000 habitantes/ano). O coeficiente de letalidade apresentou característica inversa: 13,52 óbitos para 1.000 internações/ano para o sexo masculino, contra 7,12 óbitos para 1.000 internações/ano no sexo feminino. O estado apresentou médias de valor total gasto e de valor dos serviços hospitalares de R$16.244.050,60 e R$10.890.461,31, respectivamente. A região de saúde "Capital/Vale do Gravataí" apresentou o maior valor total gasto e de serviços hospitalares, e o maior número de óbitos, média e dias de permanência. Os coeficientes de internação e de letalidade, os óbitos, os dias de permanência e os gastos referentes às internações aumentam a partir dos 50 anos de idade. O sexo feminino apresentou maior frequência e maiores valores gastos com a internação, enquanto o masculino maior coeficiente de letalidade e média de permanência hospitalar.

Evolution of microbial communities during electrokinetic treatment of antibiotic-polluted soil.

PubMed

Li, Hongna; Li, Binxu; Zhang, Zhiguo; Zhu, Changxiong; Tian, Yunlong; Ye, Jing

2018-02-01

The evolution of microbial communities during the electrokinetic treatment of antibiotic-polluted soil (EKA) was investigated with chlortetracycline (CTC), oxytetracycline (OTC) and tetracycline (TC) as template antibiotics. The total population of soil microorganisms was less affected during the electrokinetic process, while living anti-CTC, anti-OTC, anti-TC and anti-MIX bacteria were inactivated by 10.48%, 31.37%, 34.76%, and 22.08%, respectively, during the 7-day treatment compared with antibiotic-polluted soil without an electric field (NOE). Accordingly, samples with NOE treatment showed a higher Shannon index than those with EKA treatment, indicating a reduction of the microbial community diversity after electrokinetic processes. The major taxonomic phyla found in the samples of EKA and NOE treatment were Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria. And the distribution of Actinobacteria, Cyanobacteria, and Chloroflexi was greatly decreased compared with blank soil. In the phylum Proteobacteria, the abundance of Alphaproteobacteria was greatly reduced in the soils supplemented with antibiotics (from 13.40% in blank soil to 6.43-10.16% after treatment); while Betaproteobacteria and Deltaproteobacteria showed a different trend with their abundance increased compared to blank soil, and Gammaproteobacteria remained unchanged for all treatments (2.36-2.78%). The varied trends for different classes indicated that the major bacterial groups changed with the treatments due to their different adaptability to the antibiotics as well as to the electric field. SulI being an exception, the reduction ratio of the observed antibiotic resistance genes (ARGs) including tetC, tetG, tetW, tetM, intI1, and sulII in the 0-2cm soil sampled with EKA versus NOE treatment reached 55.17%, 3.59%, 99.26%, 89.51%, 30.40%, and 27.92%, respectively. Finally, correlation analysis was conducted between antibiotic-resistant bacteria, ARGs and taxonomic bacterial classes. It was found that sulII was the most representative of many different bacteria among the seven ARGs studied. This is the first report on the changes in microbial communities before and after EKA, and the present results demonstrated that the application of EKA is a useful and effective approach to suppressing both antibiotic resistant microorganisms and ARGs. Copyright © 2017 Elsevier Inc. All rights reserved.

High Prevalence of CTX-M-15-Type ESBL-Producing E. coli from Migratory Avian Species in Pakistan.

PubMed

Mohsin, Mashkoor; Raza, Shahbaz; Schaufler, Katharina; Roschanski, Nicole; Sarwar, Fatima; Semmler, Torsten; Schierack, Peter; Guenther, Sebastian

2017-01-01

The increased presence of clinically relevant multidrug resistant bacteria in natural environments is an emerging challenge for global health care. Little is known regarding the occurrence of extended-spectrum beta-lactamase producing Escherichia coli (ESBL- E. coli ) from environmental sentinels in Pakistan. The goal of the current study was to gain insights into the prevalence and phylogenetic relationships of ESBL- E. coli recovered from wild birds in Pakistan during winter migration. After initial screening of fecal samples on selective chromogenic agar, ESBL- E.coli were analyzed phenotypically using the Vitek-2 automated system. Genotypic characterization was performed using whole genome sequencing (WGS) followed by an in-depth in silico analysis. Of 150 birds screened, 26 (17.3%) were fecal carriers of ESBL- E. coli . Of these, 88.4% isolates exhibited multidrug resistance (MDR) phenotypes. Resistance to cefotaxime, ceftazidime, ampicillin, doxycycline, tetracycline and sulfamethoxazole/trimethoprim (CTX-CAZ-AM-DC-TE-SXT) represented the most common pattern of MDR (76.9%). WGS data analysis found bla CTX-M-15 as the predominant ESBL genotype (92.3%). Other genes encoding resistance to sulfonamides ( sul1/sul2/sul3 ), aminoglycosides ( strA, strB, aadA1, aadA2, aadA5, aac(3)-IId-like, aac(3)-IVa-like and aph(4)-Ia) , trimethoprim (dfrA14 or dfrA17) , tetracyclines [ tet(A)/tet(B) ], and fluoroquinolones ( qnr S1) were detected commonly, often encoded on IncF-type plasmids (76.9%). ESBL- E. coli were assigned to 17 different sequence types (STs) of which ST10 and ST7097 (4 isolates each) were the most abundant followed by ST4720, ST93, and ST1139 (2 isolates each). Core-genome phylogeny of the isolates found low numbers (0-29) of single nucleotide polymorphisms (SNPs) in isolates belonged to ST7097 originated from two different locations (Chashma barrage and Rasul barrage). Similar trends were found among isolates belong to ST1139. In addition, WGS-based plasmid typing and S1-digestion found plasmids of the same pMLST type (IncF[F-:A-:B53]) and similar sizes in different bacterial and avian hosts suggesting horizontal gene transfer as another possibility for the spread of ESBL- E. coli in avian wildlife in Pakistan.

Isolation, Antimicrobial Susceptibility Profile and Detection of Sul1, blaTEM, and blaSHV in Amoxicillin-Clavulanate-Resistant Bacteria Isolated From Retail Sausages in Kampar, Malaysia

PubMed Central

Tew, Lih-Shin; She, Li-Yen; Chew, Choy-Hoong

2016-01-01

Background Due to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern. Objectives In this study, we aimed to detect the presence and discover the molecular determinants of foodborne bacteria in retail sausages resistant towards the antibacterial agent amoxicillin-clavulanate. Methods Two grams of sausages were chopped into small pieces and transferred into sterile Luria-Bertani (LB) enrichment broths overnight before they were plated on MacConkey agar petri dishes. The bacteria isolated were then screened for amoxicillin-clavulanate resistance, and an antimicrobial susceptibility test of each isolate was performed by using the disc diffusion method. Double synergy and phenotypic tests were carried out to detect the presence of extended spectrum β-lactamase (ESBL). API 20E kit was used to identify the Enterobacteriaceae. All isolates were further examined by polymerase chain reaction (PCR) for resistant genes blaOXA-1, blaOXA-10, plasmid-mediated AmpC (blaCMY and blaDHA), and the chromosome-mediated AmpC, Sul1, blaTEM, and blaSHV genes. Results A total of 18 amoxicillin-clavulanate resistant isolates were obtained from seven different types of retail sausages. Only half of them were identified as Enterobacteriaceae, but none were ESBL-producers. All the 18 isolated strains demonstrated resistance towards amoxicillin-clavulanate, penicillin and oxacillin (100%), cefotaxime (71.4%), cefpodoxime (66.7%), and ampicillin (83.3%). blaTEM was the most frequently detected β-lactamase gene. Both plasmid- and chromosomal-bound blaTEM genes were detected in all of the isolated Enterobacteriaceae. blaSHV and Sul1 accounted for 22.2% and 11.1% of the amoxicillin-clavulanate resistant isolates, respectively, whereas blaAMPC, blaCMY, blaDHA, blaOXA-1, and blaOXA-10 were not found in any of the isolates. The only one ESBL-producing bacteria detected in this study was Chryseobacterium meningosepticum, which harbored the blaTEM gene. Conclusions The multidrug resistant bacteria that carry antibiotic resistant genes from retail sausages may increase the risk of transmission to humans via the consumption of contaminated sausages. Stricter measures must be taken to address the use of antibiotics in animal agriculture and to consider their potential impact on human health. PMID:27942365

Isolation, Antimicrobial Susceptibility Profile and Detection of Sul1, blaTEM, and blaSHV in Amoxicillin-Clavulanate-Resistant Bacteria Isolated From Retail Sausages in Kampar, Malaysia.

PubMed

Tew, Lih-Shin; She, Li-Yen; Chew, Choy-Hoong

2016-10-01

Due to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern. In this study, we aimed to detect the presence and discover the molecular determinants of foodborne bacteria in retail sausages resistant towards the antibacterial agent amoxicillin-clavulanate. Two grams of sausages were chopped into small pieces and transferred into sterile Luria-Bertani (LB) enrichment broths overnight before they were plated on MacConkey agar petri dishes. The bacteria isolated were then screened for amoxicillin-clavulanate resistance, and an antimicrobial susceptibility test of each isolate was performed by using the disc diffusion method. Double synergy and phenotypic tests were carried out to detect the presence of extended spectrum β-lactamase (ESBL). API 20E kit was used to identify the Enterobacteriaceae . All isolates were further examined by polymerase chain reaction (PCR) for resistant genes bla OXA-1, bla OXA-10, plasmid-mediated AmpC ( bla CMY and bla DHA), and the chromosome-mediated AmpC, Sul 1, bla TEM, and bla SHV genes. A total of 18 amoxicillin-clavulanate resistant isolates were obtained from seven different types of retail sausages. Only half of them were identified as Enterobacteriaceae , but none were ESBL-producers. All the 18 isolated strains demonstrated resistance towards amoxicillin-clavulanate, penicillin and oxacillin (100%), cefotaxime (71.4%), cefpodoxime (66.7%), and ampicillin (83.3%). bla TEM was the most frequently detected β-lactamase gene. Both plasmid- and chromosomal-bound bla TEM genes were detected in all of the isolated Enterobacteriaceae . bla SHV and Sul 1 accounted for 22.2% and 11.1% of the amoxicillin-clavulanate resistant isolates, respectively, whereas bla AMPC, bla CMY, bla DHA, bla OXA-1, and bla OXA-10 were not found in any of the isolates. The only one ESBL-producing bacteria detected in this study was Chryseobacterium meningosepticum , which harbored the bla TEM gene. The multidrug resistant bacteria that carry antibiotic resistant genes from retail sausages may increase the risk of transmission to humans via the consumption of contaminated sausages. Stricter measures must be taken to address the use of antibiotics in animal agriculture and to consider their potential impact on human health.

Geographical and Temporal Variability in Susceptibility to Cry1F Toxin From Bacillus thuringiensis in Spodoptera frugiperda (Lepidoptera: Noctuidae) Populations in Brazil.

PubMed

Farias, Juliano R; Horikoshi, Renato J; Santos, Antonio C; Omoto, Celso

2014-12-01

The genetically modified maize TC1507 event with the cry1F gene (Cry1F maize) has been used to control Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in Brazil since the 2009-2010 cropping season. As part of the insect resistance management program, we conducted studies to determine the baseline susceptibility to Cry1F before the widespread planting of Cry1F maize. Subsequently, we evaluated the geographical and temporal variability of susceptibility to this toxin in populations of S. frugiperda collected from major maize-growing regions in Brazil. The baseline susceptibility to Cry1F was determined using a diet-overlay bioassay for a susceptible reference population and four field populations of S. frugiperda. We then monitored the susceptibility to Cry1F in 43 populations of S. frugiperda sampled in nine States of Brazil between 2011 and 2013. In the baseline study, the MIC50 (the concentration that inhibits molting to second instars in 50% of individuals) ranged from 3.59 to 72.47 ng Cry1F toxin per centimeter square. Based on the upper limit of the MIC99 value of the joint analysis from the baseline susceptibility data, the concentrations of 200 and 2,000 ng of Cry1F toxin per centimeter square were defined as diagnostic concentrations for potentially resistant individuals, and these were used to monitor the susceptibility of S. frugiperda to Cry1F. Survival at 2,000 ng Cry1F toxin per centimeter square increased significantly throughout the cropping seasons in S. frugiperda populations from São Paulo, Santa Catarina, Rio Grande do Sul, Bahia, Mato Grosso, Goiás, Mato Grosso do Sul, and Paraná. The highest survival (>50%) was reached in populations collected from Bahia, Mato Grosso, Goiás, Mato Grosso do Sul, and Paraná during the 2012-2013 cropping season. Therefore, a significant decrease in susceptibility to Cry1F was detected in S. frugiperda throughout cropping seasons, especially in regions with intensive maize production in Brazil, according to field-evolved resistance that has been demonstrated in Brazilian populations. © 2014 Entomological Society of America.

Turbofan Engine Technology Evaluation System, User’s Guide.

DTIC Science & Technology

1984-04-01

MOL I GROUP I SUL O. Gas Turbine Engine Parametric Computer Program 2105 2101 2103I Simulation Design & off design Turbofan Engine LComputer...show the high pressure turbine and the two cooling air ducts highlighted in the engine drawing. H$ANGE UALUE (S) HIGH PRESSURE TURBINE COOLING VANE ...ducted off to be used for high and low pressure vane and rotor cooling in the turbines before it enters the burner section of the engine at station 31

Gymnotus refugio, a new and endangered species of electric fish of the Gymnotus pantherinus species-group from southern Brazil (Gymnotiformes: Gymnotidae).

PubMed

Giora, Julia; Malabarba, Luiz Roberto

2016-01-19

Gymnotus refugio, new species, is described from coastal rivers of Rio Grande do Sul and Santa Catarina states, Brazil. It is diagnosed from most species of the Gymnotus pantherinus species-group by the coloration pattern, further differing from G. pantherinus and G. capitimaculatus on the basis of morphometric and meristic characters. The new species represents the most meridional record of the G. pantherinus species-group.

Worldwide Report, Telecommunications Policy, Research and Development, No. 258

DTIC Science & Technology

1983-01-28

3379, December 1979. [20] F. Vatalaro: Caratteristiche di un filtro digitale dpplicato al sistema DME di precisione per MLS, F.U.B., Relazione 43...Spring 1980. [8] F. Chiarini, M. Gori: Possibilitä di miglioramento della precisione di un DME in banda L öfferta da u’n sistema di antenne a...Vecchio, G. Pedrini, F. Vatalaro: Rapporto sul programma per la determinazione delta forma d’onda impulsiva ottima per un sistema DME di precisione in

Corrosion and Coatings Review of Specifications and Drawings of Facilities for the Oman Military Construction Program at Masirah Island, Oman.

DTIC Science & Technology

1984-12-01

5 Background Objective Approach Scope of Work Organization 2 SUMMARY OF ENVIRONMENTAL CONDITIONS AT MASIRAH ISLAND ................. 7 Soil ...CONDITIONS AT MASIRAH ISLAND Soil Resistivity Values for the soil resistivity at this location are not readily avail- able. It is, however, considered...to be a very aggressive soil with high amounts of chlorides and sulfates, which can be converted to corrosive sul- -. fides. Soil samples will be

Pope AFB, Fayetteville, North Carolina Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

DTIC Science & Technology

1984-06-01

TEMPERATURE DEPRESION (P) ITOTAL TOTAL ___ (F) * 1 .2 3.4 9.6 7-L 9.10 11.121* 1 3-1 I5 171,,IV.2021.2223.,42S.2627.209.2S 831 .S..SDyBb hWet b.Ib...WEATHIER SERVICE/MAC 723030 POPE AFB MC STINNM74-83 vm * PAGE I wnEDnn. T.... ____ ___ WET SUL& TEMPERATURE DEPRESION (F) TOTAL ___TOTAL *(F) 0 1.2

MM&T: Bibliography on Optical Testing with Appendix.

DTIC Science & Technology

1982-02-26

OTTENUTE CON UN RETICOLO A TRATTI INCLINATI SUL PIANO DE SIM1ETRIA DEL SISTEMA OTTICO" Nuovo Cimento, 8, 310 (1931) No abstract provided. SCANDONE, F...FRANCIA, G. "SULLA FRANGE D’INTERFERENZA DELLE ONDE ABERRANTI" Ottica. 6, 151 (1941) No abstract provided. TORALDO DI FRANCIA, G. "SAUGIO DI UNA TEORIA ...No abstract provided. TORALDO DI FRANCIA, G. "INTRODUZIONE ALIA TEORIA GEOMETR[CA E INTERFERENZIALE DELLE ONDE ABERRANTI" Atti. Fond. Giorgio Ronchi

[The prevalence of nosocomial infection in Intensive Care Units in the State of Rio Grande do Sul].

PubMed

Lisboa, Thiago; Faria, Mario; Hoher, Jorge A; Borges, Luis A A; Gómez, Jussara; Schifelbain, Luciele; Dias, Fernando S; Lisboa, João; Friedman, Gilberto

2007-12-01

To determine the prevalence of intensive care unit (ICU)-acquired infections and the risk factors for these infections, identify the predominant infecting organisms, and evaluate the relationship between ICU-acquired infection and mortality. A 1-day point prevalence study. Sixteen ICU of the State of Rio Grande do Sul-Brazil, excluding coronary care and pediatric units. All patients < 12 yrs occupying an ICU bed over a 24-hour period. The 16 ICU provided 174 case reports. rates of ICU-acquired infection, resistance patterns of microbiological isolates, and potential risks factors for ICU-acquired infection and death. A total of 122 patients (71%) was infected and 51 (29%) had ICU-acquired infection. Pneumonia (58.2%), lower tract respiratory infection (22.9%), urinary tract infection (18%) were the most frequents types of ICU infection. Most frequently microorganisms reported were staphylococcus aureus (42% [64% resistant to oxacilin]) and pseudomonas aeruginosa (31%). Six risk factors for ICU acquired infection were identified: urinary catheterization, central vascular line, tracheal intubation for prolonged time (> 4 days), chronic disease and increased length of ICU stay (> 30 days). The risks factors associated with death were age, APACHE II, organ dysfunction, and tracheal intubation with or without mechanical ventilation. ICU-acquired infection is common and often associated with microbiological isolates of resistant organisms. This study may serve as an epidemiological reference to help the discussion of regional infection control policies.

Paediatric dialysis and renal transplantation in the state of Rio Grande do Sul, Brazil.

PubMed

Garcia, C; Goldani, J; Garcia, V

1992-01-01

Renal replacement therapy (RRT) for Brazilian children with uraemia has been utilized since 1970 in the state of Rio Grande do Sul. One hundred and eighty patients receiving this therapy between 1970 and 1988 have been reviewed. The annual acceptance rate of new paediatric patients in this period increased from 0.6 to 6.5 patients per million child population. Glomerulonephritis (36.1%) and pyelonephritis including urological anomalies (31.7%) were the most frequent causes of end-stage renal disease. Outpatient hospital haemodialysis was the primary form of dialytic treatment in patients 5-15 years of age. Continuous ambulatory peritoneal dialysis was more often used in patients less than 5 years of age. The survival after 1 year on dialysis was 79.9% for children aged 5-15 years starting dialysis during the period 1985-1988. Fluid overload with congestive heart failure and infection were the main causes of death in children on dialysis. Eighty-four children received 93 grafts; only 14 (15%) were from cadaveric donors. One-year patient and graft survival of first living-related donor transplants were 92.2% and 78.5% respectively during the period 1985-1988. Infection accounted for 43.5% of deaths after transplantation. We conclude that RRT is becoming increasingly successful for children in our region but that greater emphasis upon patient compliance with all forms of RRT and upon cadaver kidney donation is needed.

SAND FLIES (DIPTERA: PSYCHODIDAE) IN AN ENDEMIC AREA OF LEISHMANIASIS IN AQUIDAUANA MUNICIPALITY, PANTANAL OF MATO GROSSO DO SUL , BRAZIL

PubMed Central

de FIGUEIREDO, Helen Rezende; SANTOS, Mirella Ferreira da Cunha; CASARIL, Aline Etelvina; INFRAN, Jucelei Oliveira de Moura; RIBEIRO, Leticia Moraes; FERNANDES, Carlos Eurico dos Santos; de OLIVEIRA, Alessandra Gutierrez

2016-01-01

SUMMARY The Aquidauana municipality is considered an endemic area of leishmaniasis and an important tourist site in Mato Grosso do Sul State. The aim of this study was to investigate the sand fly fauna in the city of Aquidauana. Captures were carried out twice a month, from April 2012 to March 2014 with automatic light traps and active aspiration, in the peridomicile and domicile of six residences. A total of 9,338 specimens were collected, 3,179 and 6,159 using light traps and active aspiration, respectively. The fauna consisted of: Brumptomyia brumpti, Evandromyia aldafalcaoae, Ev. evandroi, Ev. lenti, Ev. orcyi, Ev. sallesi, Ev. termitophila, Ev. walkeri, Lutzomyia longipalpis and Psathyromyia bigeniculata. The most abundant species captured was Lutzomyia longipalpis, present in all the ecotopes, predominantly in peridomicile areas, and mainly males. Leishmania DNA was not detected in the insects. It was observed the abundance of the sand fly fauna in the region, as well as the high frequency of Lu. longipalpis, the main vector of L. infantum. The results of this study show the need to increase the monitoring and more effective control measures. It is noteworthy that the studied region presents several activities related to tourism and recreation, increasing the risk of transmission of leishmaniasis to this particular human population. PMID:27982353

Preliminary Analysis of IR Cloudtop Temperatures of Sprite Producing Storms over Argentina Observed from Brazil, and Comparison with US Case Study

NASA Astrophysics Data System (ADS)

Sao Sabbas, F. T.; Pautet, P.; Taylor, M. J.; Pinto, O.; Thomas, J.; Solorzano, N.; Holzworth, R.; Bailey, M.; Schuch, N.; Michels, M.

2006-12-01

We will present the preliminary results of the cloudtop temperature characteristics of two very active sprite- producing Mesoscale Convective System (MCS) which occurred over Argentina in the evening of February 22, and March 04, 2006. These were prolific storms, e.g. the first one produced more than 400 TLEs, including sprites, halos and possibly elves. The events were observed from the INPE Observatorio Espacial Sul-OES (Southern Space Observatory), located at the center of Rio Grande do Sul State, the most Southern State of Brazil. Except for the lack of triangulated locations for the sprites and halos recorded, the methodology used for this study is the same as for the paper Sao Sabbas and Sentman [2003], where a sprite producing storm over the central U.S. was observed during the night of July 22, 1996. We analyzed the IR satellite data provided by GOES-12 and the lightning information from the Brazilian Lightning Detection Network in combination with data from the World Wide Lightning Location Network WWLLN. We will also show a comparison between the obtained results and the results presented at the Sao Sabbas and Sentman [2003] paper. Sao Sabbas, F.T. and D. D. Sentman, Dynamical Relationship of IR Cloudtop Temperatures With Occurrence Rates of Cloud-to-Ground Lightning and Sprites, Geophys. Res. Lett., 30 (5), 40-1-40-4, 2003.

Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure

PubMed Central

Zhang, Hao; Li, Xunan; Yang, Qingxiang; Sun, Linlin; Yang, Xinxin; Zhou, Mingming; Deng, Rongzhen; Bi, Linqian

2017-01-01

Antibiotic contamination in agroecosystems may cause serious problems, such as the proliferation of various antibiotic resistant bacteria and the spreading of antibiotic resistance genes (ARGs) in the environment or even to human beings. However, it is unclear whether environmental antibiotics, antibiotic resistant bacteria, and ARGs can directly enter into, or occur in, the endophytic systems of plants exposed to pollutants. In this study, a hydroponic experiment exposing pakchoi (Brassica chinensis L.) to tetracycline, cephalexin, and sulfamethoxazole at 50% minimum inhibitory concentration (MIC) levels and MIC levels, respectively, was conducted to explore plant growth, antibiotic uptake, and the development of antibiotic resistance in endophytic systems. The three antibiotics promoted pakchoi growth at 50% MIC values. Target antibiotics at concentrations ranging from 6.9 to 48.1 µg·kg−1 were detected in the treated vegetables. Additionally, the rates of antibiotic-resistant endophytic bacteria to total cultivable endophytic bacteria significantly increased as the antibiotics accumulated in the plants. The detection and quantification of ARGs indicated that four types, tetX, blaCTX-M, and sul1 and sul2, which correspond to tetracycline, cephalexin, and sulfamethoxazole resistance, respectively, were present in the pakchoi endophytic system and increased with the antibiotic concentrations. The results highlight a potential risk of the development and spread of antibiotic resistance in vegetable endophytic systems. PMID:29099753

Biofilms as a sink for antibiotic resistance genes (ARGs) in the Yangtze Estuary

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guo, Xing-pan; Yang, Yi; Lu, Da-pei

Biofilms are ubiquitous throughout aquatic environments and they are thought to promote the acquisition and dissemination of antibiotic resistant genes (ARGs). This study focused on the occurrence and distribution of five types of ARG in naturally-occurring biofilms, in comparison to associated sediment and water samples, from the Yangtze Estuary, which borders the meta-city of Shanghai, China. The detection frequency and abundances of most ARGs showed the following order: biofilm > sediment > water, which can be attributed to a high level of antibiotics and metals that can accelerate the generation and propagation of ARGs in biofilms. Most of ARG abundancesmore » were contributed by extracellular DNA (eDNA) in biofilm and sediment samples. ARGs (sul1, sul2, tetAandtetW) in eDNA were significantly correlated with TOC in both biofilm and sediment samples. Furthermore, both intracellular DNA-associated ARGs per gram of microbial biomass carbon (MBC) and eDNA-associated ARGs per gram of non-MBC and were higher in biofilms than sediments, and the partitioning coefficients of ARGs in eDNA between biofilm and water were higher than those between sediment and water. Our results provide new insight for evaluating the occurrence and abundance of ARGs in aquatic environments, confirming that biofilms are a significant sink for ARGs in the estuarine environment.« less

Metagenomic Characterization of Antibiotic Resistance Genes in Full-Scale Reclaimed Water Distribution Systems and Corresponding Potable Systems.

PubMed

Garner, Emily; Chen, Chaoqi; Xia, Kang; Bowers, Jolene; Engelthaler, David M; McLain, Jean; Edwards, Marc A; Pruden, Amy

2018-06-05

Water reclamation provides a valuable resource for meeting nonpotable water demands. However, little is known about the potential for wastewater reuse to disseminate antibiotic resistance genes (ARGs). Here, samples were collected seasonally in 2014-2015 from four U.S. utilities' reclaimed and potable water distribution systems before treatment, after treatment, and at five points of use (POU). Shotgun metagenomic sequencing was used to profile the resistome (i.e., full contingent of ARGs) of a subset ( n = 38) of samples. Four ARGs ( qnrA, bla TEM , vanA, sul1) were quantified by quantitative polymerase chain reaction. Bacterial community composition (via 16S rRNA gene amplicon sequencing), horizontal gene transfer (via quantification of intI1 integrase and plasmid genes), and selection pressure (via detection of metals and antibiotics) were investigated as potential factors governing the presence of ARGs. Certain ARGs were elevated in all ( sul1; p ≤ 0.0011) or some ( bla TEM , qnrA; p ≤ 0.0145) reclaimed POU samples compared to corresponding potable samples. Bacterial community composition was weakly correlated with ARGs (Adonis, R 2 = 0.1424-0.1734) and associations were noted between 193 ARGs and plasmid-associated genes. This study establishes that reclaimed water could convey greater abundances of certain ARGs than potable waters and provides observations regarding factors that likely control ARG occurrence in reclaimed water systems.

Topology mapping to characterize cyanobacterial bicarbonate transporters: BicA (SulP/SLC26 family) and SbtA.

PubMed

Price, G Dean; Howitt, Susan M

2014-09-01

This mini-review addresses advances in understanding the transmembrane topologies of two unrelated, single-subunit bicarbonate transporters from cyanobacteria, namely BicA and SbtA. BicA is a Na(+)-dependent bicarbonate transporter that belongs to the SulP/SLC26 family that is widespread in both eukaryotes and prokaryotes. Topology mapping of BicA via the phoA/lacZ fusion reporter method identified 12 transmembrane helices with an unresolved hydrophobic region just beyond helix 8. Re-interpreting this data in the light of a recent topology study on rat prestin leads to a consensus topology of 14 transmembrane domains with a 7+7 inverted repeat structure. SbtA is also a Na(+)-dependent bicarbonate transporter, but of considerably higher affinity (Km 2-5 μM versus >100 μM for BicA). Whilst SbtA is widespread in cyanobacteria and a few bacteria, it appears to be absent from eukaryotes. Topology mapping of SbtA via the phoA/lacZ fusion reporter method identified 10 transmembrane helices. The topology consists of a 5+5 inverted repeat, with the two repeats separated by a large intracellular loop. The unusual location of the N and C-termini outside the cell raises the possibility that SbtA forms a novel fold, not so far identified by structural and topological studies on transport proteins.

Contextual determinants of neonatal mortality using two analysis methods, Rio Grande do Sul, Brazil.

PubMed

Zanini, Roselaine Ruviaro; Moraes, Anaelena Bragança de; Giugliani, Elsa Regina Justo; Riboldi, João

2011-02-01

To analyze neonatal mortality determinants using multilevel logistic regression and classic hierarchical models. Cohort study including 138,407 live births with birth certificates and 1,134 neonatal deaths recorded in 2003, in the state of Rio Grande do Sul, Southern Brazil. The Information System on Live Births and mortality records were linked for gathering information on individual-level exposures. Sociodemographic data and information on the pregnancy, childbirth care and characteristics of the children at birth were collected. The associated factors were estimated and compared by traditional and multilevel logistic regression analysis. The neonatal mortality rate was 8.19 deaths per 1,000 live births. Low birth weight, 1- and 5-minute Apgar score below eight, congenital malformation, pre-term birth and previous fetal loss were associated with neonatal death in the traditional model. Elective cesarean section had a protective effect. Previous fetal loss did not remain significant in the multilevel model, but the inclusion of a contextual variable (poverty rate) showed that 15% of neonatal mortality variation can be explained by varying poverty rates in the microregions. The use of multilevel models showed a small effect of contextual determinants on the neonatal mortality rate. There was found a positive association with the poverty rate in the general model, and the proportion of households with water supply among preterm newborns.

A new third-order sequence stratigraphic framework applied to the Triassic of the Paraná Basin, Rio Grande do Sul, Brazil, based on structural, stratigraphic and paleontological data

NASA Astrophysics Data System (ADS)

Horn, B. L. D.; Melo, T. M.; Schultz, C. L.; Philipp, R. P.; Kloss, H. P.; Goldberg, K.

2014-11-01

The Santacruzodon assemblage zone was originally defined as a vertebrate fossil assemblage composed basically of non-mammalian cynodonts found in Santa Cruz do Sul and Venâncio Aires municipalities in Southern Brazil. This assemblage zone was positioned at the top of the Sequence I, in the Triassic Santa Maria Supersequence, Paraná Basin. However, the Santacruzodon assemblage zone does not occur across the entire area of the Santa Maria Supersequence. Based on new paleontological, structural and sedimentological data, we propose the existence of a new third-order sequence (Santa Cruz Sequence) between Sequences I and II in the Santa Maria Supersequence. Satellite image analysis was used to identify regional, NW- and NE-oriented lineaments that limit the occurrence zone. Outcrop data allowed the identification of a regional, angular unconformity that bounds the new sequence. The faunal content allowed the correlation of the new Santa Cruz Sequence with Madagascar's Isalo II fauna, corresponding to the Ladinian (Middle Triassic). New names were suggested for the sequences in the Santa Maria Supersequence, since the Santa Cruz Sequence was deposited between the former Sequences I and II. This unit was deposited or preserved exclusively on the hanging wall of normal faults, being absent from the adjacent structural blocks.

South Brazilian wines: culturable yeasts associated to bottled wines produced in Rio Grande do Sul and Santa Catarina.

PubMed

Ramírez-Castrillón, Mauricio; Mendes, Sandra Denise Camargo; Valente, Patricia

2017-04-01

A comprehensive understanding of the presence and role of yeasts in bottled wines helps to know and control the organoleptic quality of the final product. The South Region of Brazil is an important wine producer, and the state of "Rio Grande do Sul" (RS) accounts for 90% of Brazilian wines. The state of "Santa Catarina" (SC) started the production in 1975, and is currently the fifth Brazilian producer. As there is little information about yeasts present in Brazilian wines, our main objective was to assess the composition of culturable yeasts associated to bottled wines produced in RS and SC, South of Brazil. We sampled 20 RS and 29 SC bottled wines produced between 2003 and 2011, and we isolated culturable yeasts in non-selective agar plates. We identified all isolates by sequencing of the D1/D2 domain of LSU rDNA or ITS1-5.8 S-ITS2 region, and comparison with type strain sequences deposited in GenBank database. Six yeast species were shared in the final product in both regions. We obtained two spoilage yeast profiles: RS with Zygosaccharomyces bailii and Pichia membranifaciens (Dekkera bruxellensis was found only in specific table wines); and SC with Dekkera bruxellensis and Pichia manshurica. Knowledge concerning the different spoilage profiles is important for winemaking practices in both regions.

Evaluation of Aster Images for Characterization and Mapping of Amethyst Mining Residues

NASA Astrophysics Data System (ADS)

Markoski, P. R.; Rolim, S. B. A.

2012-07-01

The objective of this work was to evaluate the potential of Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), subsystems VNIR (Visible and Near Infrared) and SWIR (Short Wave Infrared) images, for discrimination and mapping of amethyst mining residues (basalt) in the Ametista do Sul Region, Rio Grande do Sul State, Brazil. This region provides the most part of amethyst mining of the World. The basalt is extracted during the mining process and deposited outside the mine. As a result, mounts of residues (basalt) rise up. These mounts are many times smaller than ASTER pixel size (VNIR - 15 meters and SWIR - 30 meters). Thus, the pixel composition becomes a mixing of various materials, hampering its identification and mapping. Trying to solve this problem, multispectral algorithm Maximum Likelihood (MaxVer) and the hyperspectral technique SAM (Spectral Angle Mapper) were used in this work. Images from ASTER subsystems VNIR and SWIR were used to perform the classifications. SAM technique produced better results than MaxVer algorithm. The main error found by the techniques was the mixing between "shadow" and "mining residues/basalt" classes. With the SAM technique the confusion decreased because it employed the basalt spectral curve as a reference, while the multispectral techniques employed pixels groups that could have spectral mixture with other targets. The results showed that in tropical terrains as the study area, ASTER data can be efficacious for the characterization of mining residues.

Educational Activities for Rural and Urban Students to Prevent Skin Cancer in Rio Grande do Sul, Brazil.

PubMed

Velasques, Kelle; Michels, Luana Roberta; Colome, Leticia Marques; Haas, Sandra Elisa

2016-01-01

Excessive exposure to the sun during childhood is strongly associated with the development of skin cancer in the future. The only way to prevent the development of skin cancer is to protect against ultraviolet radiation, which can be achieved through strategic awareness during childhood and adolescence. The aim of this work was to evaluate the impact of educational activities for rural and urban students to promote the use of sunscreens and prevent skin cancer. This study was carried out with students (9-12 years) of rural (n=70) and urban (n=70) schools in Rio Grande do Sul state, Brazil. The educational interventions were lectures and games. The impact of this strategy was evaluated through the application of a questionnaire before and after the interventions. Before the intervention, it was found around 50% of rural and urban students were not aware of the damage caused by sun exposure, often exposing themselves to UV radiation without use sunscreen ( ~ 25 %) and at the most critical times of the day/year. After the lectures we observed an improvement in the behavior of the students with regard to sun exposure and knowledge about skin cancer. The results of this study emphasize the importance of prevention strategies for skin cancer and promoting the use of sunscreens based educational strategies. The interventions were of great value in relation to disseminating knowledge on the subject.

[The perception of the young and long-lived elderly 'Gauchos' (from the State of Rio Grande do Sul, Brazil) about the public spaces they live in Resumo].

PubMed

Navarro, Joel Hirtz do Nascimento; Andrade, Francini Porcher; Paiva, Tiago Sousa; da Silva, Diovana Ourique; Gessinger, Cristiane Fernanda; Bós, Ângelo José Gonçalves

2015-02-01

By 2050, the number of Brazilians living in urban areas will be over 200 million and 29% of the population will be elderly. The long-lived elderly are 80 or more years old and the young elderly are between 60 and 79 years of age. The scope of this article was to verify the difference in perception between the young elderly and the long-lived elderly from Rio Grande do Sul (RS) about the urban environment they live in. This is a population-based, observational, descriptive, retrospective study with a quantitative analysis paradigm. Data was analyzed from Elderly Profile research in RS conducted by the Geriatric and Gerontological Institute of PUCRS in partnership with the RS School of Public Health. The sample consisted of 6913 questionnaires answered by the elderly from 59 cities. Data analysis was performed for each age group and independent variables were processed using the Chi-square test, with p under 0.05. Results showed that the perception of difficulties such as a lack of park benches and safety strips, short traffic light times for pedestrians, high steps and bad-smelling public toilets was greater among the young elderly. The long-lived elderly noticed these facts less, though they admitted that they frequent community environments less often.

[Food insecurity and inadequate food intake among students of municipal schools, São Leopoldo, state of Rio Grande do Sul, Brazil].

PubMed

Ruschel, Letícia Fialho; Henn, Ruth Liane; Backes, Vanessa; Melo, Priscila de; Marques, Luana Araújo da Silva; Olinto, Maria Teresa Anselmo

2016-06-01

This research involves a school-based cross sectional study to evaluate the association between food insecurity (FI) and inadequate food intake (IFI), among 782 children (mean age of 6.9±0.5) of the 1st year of primary school in the municipal schools of São Leopoldo in the state of Rio Grande do Sul. Data were gathered from mothers or guardians. FI was obtained through the Brazilian Food Insecurity Scale, and food consumption using the food frequency questionnaire. Foods were classified as healthy eating markers (HEM) and unhealthy (UEM) and received scores according to intake frequency. HEM: 0 - 1 day - zero; 2 to 3 days - 0.25; 4 to 5 days - 0.75; 6 to 7 days - 1, and the reverse for the UEM. The scores could range from 0 to 25 (HEMS) and 0 to 19 (UEMS). A standardized total score (TS) was obtained [HEMS * (19/44) + UEMS * (25/44)] and could range from 0 to 22. The scores were categorized into terciles and the 1st considered IFI. The FI was 45.1% and the average scores were 5.9 (HEMS), 15.9 (UEMS) and 11.6 (TS). No association was found between IA and IFI. High IA and low food scores require intersectoral approaches to ensure access to food in quantity and quality appropriate for this population.

Morphometric Analysis of Longipalpis (Diptera: Psychodidae) Complex Populations in Mato Grosso do Sul, Brazil.

PubMed

Santos, Mirella F C; Andrade Filho, José D; Fernandes, Carlos E S; Mateus, Nathália L F; Eguchi, Gabriel U; Fernandes, Wedson D; Brazil, Reginaldo P; Oliveira, Everton F; Oliveira, Alessandra G

2015-05-01

Owing to the existence of cryptic species that are difficult to distinguish morphologically, the search for new taxonomic characters and methods for identifying and classifying sand flies continues. Lutzomyia longipalpis (Lutz & Neiva, 1912) and Lutzomyia cruzi (Mangabeira, 1938) (Diptera: Psychodidae) are two such species that occur in sympatry in some regions of Mato Grosso do Sul State (MS). Twenty females and twenty males from each of the five populations of Lu. longipalpis and one population of Lu. cruzi from MS were examined. An outlying population of Lu. longipalpis from Estrela de Alagoas, State of Alagoas, was used to compare the degree of divergence among the groups in MS. Specimens were cleared, mounted on slides, identified, and measured using LAS-Leica. The principal component analysis of morphometric characters showed a high degree of variation among females, while males varied to a lower degree. The populations of Alagoas and Miranda demonstrated the greatest variation. The first region, Alagoas, is geographically distant from the others and occurs under distinctly different ecological conditions, which likely accounts for the variation. Further studies should be made to elucidate the factors that contribute to the differences found between the populations of MS. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Plant Growth, Antibiotic Uptake, and Prevalence of Antibiotic Resistance in an Endophytic System of Pakchoi under Antibiotic Exposure.

PubMed

Zhang, Hao; Li, Xunan; Yang, Qingxiang; Sun, Linlin; Yang, Xinxin; Zhou, Mingming; Deng, Rongzhen; Bi, Linqian

2017-11-03

Antibiotic contamination in agroecosystems may cause serious problems, such as the proliferation of various antibiotic resistant bacteria and the spreading of antibiotic resistance genes (ARGs) in the environment or even to human beings. However, it is unclear whether environmental antibiotics, antibiotic resistant bacteria, and ARGs can directly enter into, or occur in, the endophytic systems of plants exposed to pollutants. In this study, a hydroponic experiment exposing pakchoi ( Brassica chinensis L.) to tetracycline, cephalexin, and sulfamethoxazole at 50% minimum inhibitory concentration (MIC) levels and MIC levels, respectively, was conducted to explore plant growth, antibiotic uptake, and the development of antibiotic resistance in endophytic systems. The three antibiotics promoted pakchoi growth at 50% MIC values. Target antibiotics at concentrations ranging from 6.9 to 48.1 µg·kg -1 were detected in the treated vegetables. Additionally, the rates of antibiotic-resistant endophytic bacteria to total cultivable endophytic bacteria significantly increased as the antibiotics accumulated in the plants. The detection and quantification of ARGs indicated that four types, tet X, bla CTX-M , and sul 1 and sul 2, which correspond to tetracycline, cephalexin, and sulfamethoxazole resistance, respectively, were present in the pakchoi endophytic system and increased with the antibiotic concentrations. The results highlight a potential risk of the development and spread of antibiotic resistance in vegetable endophytic systems.

Facial profile esthetic preferences: perception in two Brazilian states.

PubMed

Oliveira, Marina Detoni Vieira de; Silveira, Bruno Lopes da; Mattos, Cláudia Trindade; Marquezan, Mariana

2015-01-01

The aim of this study was to assess the regional influence on the perception of facial profile esthetics in Rio de Janeiro state (RJ) and Rio Grande do Sul state (RS), Brazil. Two Caucasian models, a man and a woman, with balanced facial profiles, had their photographs digitally manipulated so as to produce seven different profiles. First year dental students (laypeople) assessed the images and classified them according to their esthetic preference. The result of the t test for independent samples showed differences among states for certain facial profiles. The female photograph identified with the letter 'G' (mandibular retrusion) received higher scores in RS state (p = 0.006). No differences were found for male photographs (p > 0.007). The evaluators' sex seemed not to influence their esthetic perception (p > 0.007). Considering all evaluators together, ANOVA/Tukey's test showed differences among the profiles (p ≤ 0.05) for both male and female photographs. The female photograph that received the highest score was the one identified with the letter 'F' (dentoalveolar bimaxillary retrusion/ straight profile). For the male profiles, photograph identified with the letter 'E' (dentoalveolar bimaxillary protrusion/ straight profile) received the best score. Regional differences were observed regarding preferences of facial profile esthetics. In Rio de Janeiro state, more prominent lips were preferred while in Rio Grande do Sul state, profiles with straight lips were favored. Class III profiles were considered less attractive.

Characterization of egg laying hen and broiler fecal microbiota in poultry farms in Croatia, Czech Republic, Hungary and Slovenia.

PubMed

Videnska, Petra; Rahman, Md Masudur; Faldynova, Marcela; Babak, Vladimir; Matulova, Marta Elsheimer; Prukner-Radovcic, Estella; Krizek, Ivan; Smole-Mozina, Sonja; Kovac, Jasna; Szmolka, Ama; Nagy, Bela; Sedlar, Karel; Cejkova, Darina; Rychlik, Ivan

2014-01-01

Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae, both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products.

Characterization of Egg Laying Hen and Broiler Fecal Microbiota in Poultry Farms in Croatia, Czech Republic, Hungary and Slovenia

PubMed Central

Videnska, Petra; Rahman, Md. Masudur; Faldynova, Marcela; Babak, Vladimir; Matulova, Marta Elsheimer; Prukner-Radovcic, Estella; Krizek, Ivan; Smole-Mozina, Sonja; Kovac, Jasna; Szmolka, Ama; Nagy, Bela; Sedlar, Karel; Cejkova, Darina; Rychlik, Ivan

2014-01-01

Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae, both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products. PMID:25329397

Antibiotic pollution in the Katari subcatchment of the Titicaca Lake: Major transformation products and occurrence of resistance genes.

PubMed

Archundia, D; Duwig, C; Lehembre, F; Chiron, S; Morel, M-C; Prado, B; Bourdat-Deschamps, M; Vince, E; Aviles, G Flores; Martins, J M F

2017-01-15

An increasing number of studies pointed out the ubiquitous presence of medical residues in surface and ground water as well as in soil compartments. Not only antibiotics can be found in the environment but also their transformation products about which little information is generally available. The development of bacterial resistance to antibiotics is particularly worrying as it can lead to sanitary and health problems. Studies about the dissemination of antibiotics and associated resistances in the Bolivian Altiplano are scarce. We provide baseline information on the occurrence of Sulfamethoxazole (SMX) and Trimethoprim (TMP) antibiotics as well as on the most common human SMX transformation products (TP) and on the occurrence of sulfonamide resistance genes. The studied water and soil compartments presented high levels of antibiotic pollution. This situation was shown to be mainly linked with uncontrolled discharges of treated and untreated wastewaters, resulting on the presence of antibiotics in the Titicaca Lake. SMX TPs were detected in surface waters and on soil sampled next to the wastewater treatment plant (WWTP). SMX resistance genes sulI and sulII were widely detected in the basin hydrological network, even in areas unpolluted with antibiotics. Mechanisms of co-selection of antibiotic- and metal- resistance may be involved in the prevalence of ARG's in pristine areas with no anthropogenic activity and free of antibiotic pollution. Copyright © 2016 Elsevier B.V. All rights reserved.

Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters.

PubMed

Norman, Anders; Hestbjerg Hansen, Lars; Sørensen, Søren J

2005-05-01

Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) revealed that the promoter for the ColD plasmid-borne cda gene had responses 12, 5, and 3 times greater than the recA, sulA, and umuDC promoters, respectively, and also considerably higher sensitivity. Furthermore, we showed that when the SOS-GFP construct was introduced into an E. coli host deficient in the tolC gene, the minimal detection limits toward mitomycin C, MNNG, nalidixic acid, and formaldehyde were lowered to 9.1 nM, 0.16 microM, 1.1 microM, and 141 microM, respectively, which were two to six times lower than those in the wild-type strain. This study thus presents a new SOS-GFP whole-cell biosensor which is not only able to detect minute levels of genotoxins but, due to its use of the green fluorescent protein, also a reporter system which should be applicable in high-throughput screening assays as well as a wide variety of in situ detection studies.

Effects of flooding of the River Paraná on the temporal activity of Anopheles (Nyssorhynchus) darlingi Root (Diptera: Culicidae), at the border state of Mato Grosso do Sul and São Paulo, Brazil.

PubMed

Gomes, Almério de Castro; Paula, Marcia Bicudo de; Natal, Delsio; Gotlieb, Sabina Léa Davidson; Mucci, Luis Filipe

2010-01-01

Study of the temporal activity of malaria vectors during the implantation of a hydroelectric power station on the River Paraná, intended to generate electrical energy. The river separates the States of São Paulo and Mato Grosso do Sul, in Brazil. The objective was to verify whether alterations occurred in the wealth and diversity indices of Anopheles, following two successive floods, extended to the temporal activity and nycthemeral rhythm followed over a five year period. Mosquito capture was performed monthly using the Human Attraction Technique and Shannon Traps. The first, executed for 24h, provided the nycthemeral rhythm and the second, lasting 15 h, permitted the tracking of Anopheles during the two floods. The bimodal pattern of Anopheles darlingi defined before these floods was modified throughout the environment interventions. The same effect had repercussions on the populations of An albitarsis s.l., An triannulatus and An galvaoi. Activity prior to twilight was less affected by the environment alterations. The dam construction provoked changes in Anopheles temporal activity patterns, permitting classification of the area as an ecologically steady and unstable situation. Differences observed in Anopheles behavior due to the capture methods revealed the influence of solo and multiple attractiveness inside the populations studied.

Epidemiological findings and laboratory evaluation of sporotrichosis: a description of 103 cases in cats and dogs in southern Brazil.

PubMed

Madrid, Isabel Martins; Mattei, Antonella Souza; Fernandes, Cristina Gevehr; Nobre, Márcia de Oliveira; Meireles, Mário Carlos Araújo

2012-04-01

Sporotrichosis is a subcutaneous mycosis, which affects mainly small animals, and is considered an important public health disease. This paper describes the epidemiological and laboratory characteristics of 103 clinical cases of sporotrichosis diagnosed over a 10-year period in southern Brazil. The 92 cats and 11 dogs from eight municipalities in Rio Grande do Sul State developed especially the disseminated cutaneous and fixed cutaneous forms of the disease. Respiratory signs such as sneezing, serous nasal discharge and dyspnea were found in about 57% of the animals. The detection of Sporothrix schenckii in different clinical samples showed highest isolation in testicles (46.6%), oral cavity (45.2%) and conjunctival mucosa (38.1%). A differentiated histological pattern was found between the fixed cutaneous and disseminated cutaneous (DC) manifestations of the disease; well-organized granulomas of nodular distribution and various fungal structures prevailed in the DC form in cats. Melanin detection in S. schenckii cells by the Fontana-Masson technique was positive in 45.4% of the samples. The study revealed that the State of Rio Grande do Sul is an endemic sporotrichosis area and demonstrated the possibility of involvement of other pathways in the infection and spread of the disease. In addition, it emphasized the importance of laboratory tests for mycosis confirmation, especially in dogs that develop clinical manifestations without the presence of cutaneous lesions.

The validation & verification of an LC/MS method for the determination of total docosahexaenoic acid concentrations in canine blood serum.

PubMed

Dillon, Gerald Patrick; Keegan, Jason D; Wallace, Geoff; Yiannikouris, Alexandros; Moran, Colm Anthony

2018-06-01

Docosahexaenoic acid (DHA), is an omega 3 fatty acid (n-3 FA) that has been shown to play a role in canine growth and physiological integrity and improvements in skin and coat condition. However, potential adverse effects of n-3 FA specifically, impaired cellular immunity has been observed in dogs fed diets with elevated levels of n-3 FA. As such, a safe upper limit (SUL) for total n-3 FAs (DHA and EPA) in dogs has been established. Considering this SUL, sensitive methods detecting DHA in blood serum as a biomarker when conducting n-3 FA supplementation trials involving dogs are required. In this study, an LC-ESI-MS/MS method of DHA detection in dog serum was validated and verified. Recovery of DHA was optimized and parallelism tests were conducted with spiked samples demonstrating that the serum matrix did not interfere with quantitation. The stability of DHA in serum was also investigated, with -80 °C considered suitable when storing samples for up to six months. The method was linear over a calibration range of 1-500 μg/mL and precision and accuracy were found to meet the requirements for validation. This method was verified in an alternative laboratory using a different analytical system and operator, with the results meeting the criteria for verification. Copyright © 2018. Published by Elsevier Inc.

Abundance of antibiotic resistance genes and bacterial community composition in wild freshwater fish species.

PubMed

Marti, Elisabet; Huerta, Belinda; Rodríguez-Mozaz, Sara; Barceló, Damià; Marcé, Rafael; Balcázar, Jose Luis

2018-04-01

This study was aimed to determine the abundance of four antibiotic resistance genes (bla TEM , ermB, qnrS and sulI), as well as bacterial community composition associated with the intestinal mucus of wild freshwater fish species collected from the Foix and La Llosa del Cavall reservoirs, which represent ecosystems with high and low anthropogenic disturbance, respectively. Water and sediments from these reservoirs were also collected and analyzed to determine the pollution level by antibiotics. The bla TEM gene was only detected in brown trout and Ebro barbel, which were collected from La Llosa del Cavall reservoir. In contrast, the sulI and qnrS genes were only detected in common carp, which were collected from the Foix reservoir. Although the ermB gene was also detected in common carp, the values were below the limit of quantification. Likewise, water and sediment samples from the Foix reservoir had higher concentrations and more classes of antibiotics than those from La Llosa del Cavall. Pyrosequencing analysis of 16S rRNA genes revealed significant differences in bacterial communities associated with the intestinal mucus of fish species. Therefore, these findings suggest that anthropogenic activities are not only increasing the pollution of aquatic environments, but also contributing to the emergence and spread of antibiotic resistance in organisms that inhabit such environments. Copyright © 2017 Elsevier Ltd. All rights reserved.

Prevalence of sulfonamide-resistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan.

PubMed

Hsu, Jih-Tay; Chen, Chia-Yang; Young, Chu-Wen; Chao, Wei-Liang; Li, Mao-Hao; Liu, Yung-Hsin; Lin, Chu-Ming; Ying, Chingwen

2014-07-30

Antibiotics are commonly used in swine feed to treat and prevent disease, as well as to promote growth. Antibiotics released into the environment via wastewater could accelerate the emergence of antibiotic-resistant bacteria and resistance genes in the surrounding environment. In this study, we quantified the occurrence of sulfonamides, sulfonamide-resistant microorganisms and resistance genes in the wastewater from a swine farm in northern Taiwan and its surrounding natural water bodies and soils. Sulfonamide levels were similar in the receiving downstream and upstream river water. However, the prevalence of sulfonamide-resistant bacteria and resistance genes, as analyzed by cultivation-dependent and -independent molecular approaches, was significantly greater in the downstream compared to the upstream river water samples. Barcoded-pyrosequencing revealed a highly diverse bacterial community structure in each sample. However, the sequence identity of the sulfonamide resistance gene sul1 in the wastewater and downstream environment samples was nearly identical (99-100%). The sul1 gene, which is genetically linked to class 1 integrons, was dominant in the downstream water bodies and soils. In conclusion, the increased prevalence of sulfonamide resistance genes in the wastewater from a swine farm, independent of the persistent presence of sulfonamides, could be a potential source of resistant gene pools in the surrounding environment. Copyright © 2014 Elsevier B.V. All rights reserved.

Do drying and rewetting cycles modulate effects of sulfadiazine spiked manure in soil?

PubMed

Jechalke, Sven; Radl, Viviane; Schloter, Michael; Heuer, Holger; Smalla, Kornelia

2016-05-01

Naturally occurring drying-rewetting events in soil have been shown to affect the dissipation of veterinary antibiotics entering soil by manure fertilization. However, knowledge of effects on the soil microbial community structure and resistome is scarce. Here, consequences of drying-rewetting cycles on effects of sulfadiazine (SDZ) in soil planted with Dactylis glomerata L. were investigated in microcosms. Manure containing SDZ or not was applied to the pregrown grass and incubated for 56 days in a climate chamber. Water was either added daily or reduced during two drying events of 7 days, each followed by a recovery phase. Total community DNA was analyzed to reveal the effects on the bacterial community structure and on the abundance of sul1, sul2, intI1 ,intI2, qacE+qacEΔ1, traN and korB genes relative to 16S rRNA genes. 16S rRNA gene-based DGGE fingerprints indicated that drying-rewetting cycles modulated the effects of SDZ on the bacterial community structure in the soil. Furthermore, the SDZ treatment increased the relative abundance of sulfonamide resistance and integrase genes compared to the control. However, this increase was not different between moisture regimes, indicating that drying-rewetting had only a negligible effect on the selection of the resistome by SDZ in the manured soil. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Evaluation of sperm quality of Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae).

PubMed

Silva, A C; Varela, A S; Cardoso, T F; Silva, E F; Loebmann, D; Corcini, C D

2017-01-01

Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus.

Ecology of the parasitic endohelminth community of Pseudoplatystoma fasciatum (Linnaeus, 1776) (Siluriformes: Pimelodidae) from the Aquidauana River, Pantanal, State of Mato Grosso do Sul, Brazil.

PubMed

Campos, C M; Fonseca, V E; Takemoto, R M; Moraes, F R

2009-02-01

This study investigated the structure and diversity of the endohelminth community and its interactions with Pseudoplatystoma fasciatum, caught in the Aquidauana River, in the State of Mato Grosso do Sul, Brazil. Ten helminth species were represented in 1,228 specimens of parasites found in the intestine and mesentery of 33 specimens of P. fasciatum. Cestodes were observed in the intestine, while nematodes Cucullanus sp. in the mesentery. Contracaecum sp. Type 1, Spatulifer rugosa and Choanoscolex abscisus showed the highest mean intensity and mean abundance and Nomimoscolex sudobin showed the highest prevalence. Simpson's index indicated dominance in the endohelminth infracommunities (C = 1.0792) and Choanoscolex abscisus was considered the central species. A clumped pattern of dispersion according to Green's index was related. 69.69% of hosts analyzed had between 2 and 5 species of endohelminths. Mean diversity was H = 0.5517 (SD = 0.4209). Two pairs of species showed significant positive association and four pairs presented significant positive correlation among abundance data. Significant negative correlations between total length and prevalence and abundance of Peltydocotyle rugosa and Nomimoscolex sudobim were found. However, no significant correlation was observed between condition factor and abundance, as well as total length and diversity. There was significant prevalence of Harriscolex kaparari in male hosts.

Paleoenvironmental evolution based on benthic foraminifera biofacies of the Paraíba do Sul Deltaic Complex, eastern Brazil

NASA Astrophysics Data System (ADS)

Gasparini, Sarah Pereira; Vilela, Claudia Gutterres

2017-12-01

The paleoecology and distribution of benthic foraminiferal assemblages were analyzed in the core 2-MU-1-RJ well, drilled in the Paraíba do Sul Deltaic Complex, Rio de Janeiro (Brazil). An abundant assemblage was found in the upper portion of the well core, inferred to be pleistocenic deposits. The coastal dynamic was recognized from five biofacies based on clusters, the Planktonic/Benthic (P/B) ratios and indicator species distribution in the core. Several biofacies were identified along the core depending on the species dominance. From the bottom to the top of the core, the biofacies succession represents the environmental changes in the coastal area associated to sea-level oscillations. The biofacies ABP dominated by Ammonia parkinsoniana and Bolivina spp. and Pararotalia cananeiaensis represents an inner shelf environment; biofacies QP dominated by shelf miliolids species; biofacies PGH, dominated by P. cananeiaensis, Gavelinopsis praegeri, and Hanzawaia nitidula, represents the estuary complex with middle or outer shelf influence; biofacies QL represents hypersaline waters dominated by lagoonal miliolids; and biofacies HP characterized by Haynesina germanica and P. cananeiaensis is associated with paralic environments. Marine ingressions are recorded and those biofacies show the pleistocenic coastal hydrodinamic in the deltaic complex. The foraminiferal biofacies contribute with detailed information to sedimentary facies previously characterized in the study area by the reconstruction of paleoenvironment succession.

A Numerical Study of the Plata River Plume Along the Southeastern South American Continental Shelf

DTIC Science & Technology

2005-01-01

Aires Depto. de Oceanografia, Servicio de Hidrografia Naval and Depto. CiEncias de la Atm6sfera y los Ocdanos, Buenos Aires, Argentina ABSTRACT The...descarrega no oceano Aguas de uma bacia de drenagem que cobre uma ampla Area da Amdrica do Sul. Sua pluma extende-se ao longo do norte da Argentina ...Instituto Nacional de Investigaci6n y Orlanski, I. 1976. A simple boundary condition for Desarrollo Pesquero, 109pp. unbounded hyperbolic flows. J

Department of the Army Supply Bulletin, Army Medical Department Supply Information, SB8-75-S3

DTIC Science & Technology

2002-03-20

CITRATE INJ R 6505011282441 METHADONE HCL TABS100 R 6505011403199 ALPRAZOLAM TABS 100S Q 6505011403200 ALPRAZOLAM 1MG 100S Q 6505011403201... ALPRAZOLAM TABS 100S Q 6505011403202 ALPRAZOLAM TABS IS100 Q 6505011439269 ALPRAZOLAM TAB 100S Q 6505011461137 OXAZEPAM CAPS10MG100S Q 6505011468044...6505011932690 SUFENTANIL CITRATE IN R 6505011938484 MORPHINE SUL SOL120ML R 6505011947256 SUFENTANIL CITRATE IN R 6505011969501 ALPRAZOLAM TABS 500S Q

A Comparison of Long-Duration Secondary-Power Schemes for Space Vehicles

DTIC Science & Technology

1959-02-02

to:" 3. Iridium . 19_ 7I? d 2.454 5,300 Beta. eak G- ’al 1.000 4.52 10’ N SulIphur -35 b7 W8 119 445 BtLA I1.00 3.72 IS Availat’Nt, _i es 5 Calo1.r...BROMIDE 28 84 41 tar area. BISMUrTH TRICHLORIDE 435 826 99 ZINC CHLORIDE 541 1349 378 case of a space power system, this receiving tem- Working-Fluid

Neolasioptera pantaneira, a new species of Cecidomyiidae (Diptera) associated with Aeschynomene denticulata (Fabaceae) from Brazil.

PubMed

Maia, V C; Catian, G; Leme, F M

2017-03-01

A new species of gall midge that induces stem galls on Aeschynomene denticulata (Fabaceae) is described based on material collected in Pantanal (Mato Grosso do Sul, Brazil). This species is unique among the Brazilian congeners in having spatula with three convex teeth, cruciate, with defined anterolateral extensions, four lateral papillae on each side of the spatula, and three pairs of terminal papillae (larva), four-segmented palpi (adult), 17 flagellomeres (female), ovipositor about 11 times length of seventh tergite.

JPRS Report, Science & Technology, Europe & Latin America, Brazil: Selections from the 1987 Federal S&T Budget.

DTIC Science & Technology

1988-02-24

of Goias 67 . Federal University of the State of Rio de Janeiro 68 . Federal University of Juiz de Fora 68 . Federal University of Minas Gerais...Federal University of Rio Grande do Norte 72 . Federal University of Rio Grande do Sul • 72 . Federal University of Rio de Janeiro 73...University of Rio de Janeiro 75 . School of Agrarian Sciences of Para 76 . School of Medicine of the Minas Triangle 77 . Federal Technological Education

Models in Insurance: Paradigms, Puzzles, Communications and Revolutions.

DTIC Science & Technology

1980-06-01

80.7] G. Buoro, G. Pavesi and G. Zucchiatti, "Osservazioni sul Sistema di Calcolo del Margine di Solvabiliti," 71-80. [80.8] J. Calcanis and A...Vegas, "Un Ensayo sobre la Concepci6n Sistema aplicada a la Empresa de Seguros," 443-462. [80.41] K. H. Wolff, "Zur numerischen Berechnung der... Teoria della Credibilit&," Giornale dell’ Istituto degli Attuari, 27, 219-231 (1964). (D51 N. De Pril, "The Efficiency of a Bonus-Malus System," AB, 10

Factors associated with postpartum weight retention in a Brazilian cohort.

PubMed

Zanotti, Joana; Capp, Edison; Wender, Maria Celeste Osório

2015-04-01

To identify the factors associated with weight retention after pregnancy. A cohort study was performed with 145 women receiving maternity care at a hospital in Caxias do Sul, Rio Grande do Sul, Brazil, aged 19 to 45 years, between weeks 38 and 42 of pregnancy. The patients were evaluated at one month, three months, and six months after delivery. Student's t-test or one-way analysis of variance (ANOVA) was used to compare groups, as indicated; correlations were assessed with Pearson's and Spearman's tests, as indicated; to identify and evaluate confounders independently associated with total weight loss, a multivariate linear regression analysis was performed and statistical significance was set at p ≤ 0.05. There was a significant positive association between total weight gain - and a negative association with physical exercise during pregnancy - with total weight loss. Higher parity, inter-pregnancy interval, calorie intake, pre-pregnancy body mass index (BMI), weight gain related to pre-pregnancy BMI, presence and severity of depression, and lack of exclusive breastfeeding were directly associated with lower weight loss. Among nominal variables, level of education and marital status were significantly associated with total weight loss. In the present study, lower weight retention in the postpartum period was associated with higher educational attainment and with being married. Normal or below-normal pre-pregnancy BMI, physical activity and adequate weight gain during pregnancy, lower parity, exclusive breastfeeding for a longer period, appropriate or low calorie intake, and absence of depression were also determinants of reduced weight retention.

[Prevalence of metabolic syndrome in indigenous people over 40 years of age in Rio Grande do Sul, Brazil].

PubMed

da Rocha, Ana Karina Silva; Bós, Angelo José Gonçalves; Huttner, Edison; Machado, Denise Cantarelli

2011-01-01

To investigate the prevalence of the metabolic syndrome (MS) among indigenous people older than 40 years of age from two cities in the State of Rio Grande do Sul, southern Brazil. A descriptive, analytic, cross-sectional study was conducted in two municipalities, Porto Alegre and Nonoai, between July and August 2009. A total of 150 indigenous people older than 40 years of age (range: 40-104 years), participated in the study. MS prevalence was determined based on National Cholesterol Education Program - Adult Treatment Panel III criteria. Blood samples and anthropometric data were collected. The participants also answered a questionnaire on eating habits, which was then contrasted to the 10 steps to healthy eating proposed by the World Health Organization and recommended by the Brazilian Ministry of Health. MS prevalence was 65.3%, affecting women more than men (P < 0.001). Changes in waist circumference, fasting glucose, and HDL-cholesterol and presence of hypertension, hypertriglyceridemia, and obesity were associated with MS. Age, smoking, and sedentary lifestyle were not associated with MS. Indigenous people with MS had a poor diet, with low intake of fruit and vegetables, low levels of physical activity, high consumption of sweets and soft drinks, and high prevalence of obesity. A high prevalence of MS was observed among the indigenous people surveyed, especially in women. Education and motivation for healthy behaviors is possibly the best way to manage MS and promote health in a population that is still neglected by public health policies.

[Association between preventive care provided in public dental services and caries prevalence].

PubMed

Celeste, Roger Keller; Nadanovsky, Paulo; De Leon, Antonio Ponce

2007-10-01

To assess the association between preventive care provided in public dental services and young people's oral health. Oral health data on 4,033 young people aged 15 to 19 years living in 85 municipalities of the state of Rio Grande do Sul, Southern Brazil, were obtained from the national oral health survey "Saúde Bucal Brasil 2003" for the period 2002-2003. The following variables were studied: age, gender, income, education, time elapsed since last dental visit, reason for dental visit, and water fluoridation. Data on dental care services were obtained from the national database of public health services. Statistical analysis was performed using multilevel logistic regression. Youngsters from the 21 municipalities with the lowest preventive care (scaling + fluoride + sealants) rates per 100 inhabitants were 2.27 (95% CI: 1.45;3.56) more likely to have non-filled dental cavities than those from the 21 municipalities with the highest care rates. After adjustment for a number of individual and contextual factors this likelihood decreased to 1.76 (95% CI: 1.13;2.72). The variance attributable to variables at municipal level was 14.1% for the empty model and decreased to 10.5% for the fully adjusted model. Rio Grande do Sul public dental services may have contributed for the reduction in the number of non-filled cavities in young people. However, it was not possible to detect the impact of this service on total dental caries experience.

Facial profile esthetic preferences: perception in two Brazilian states

PubMed Central

de Oliveira, Marina Detoni Vieira; da Silveira, Bruno Lopes; Mattos, Cláudia Trindade; Marquezan, Mariana

2015-01-01

OBJECTIVE: The aim of this study was to assess the regional influence on the perception of facial profile esthetics in Rio de Janeiro state (RJ) and Rio Grande do Sul state (RS), Brazil. METHODS: Two Caucasian models, a man and a woman, with balanced facial profiles, had their photographs digitally manipulated so as to produce seven different profiles. First year dental students (laypeople) assessed the images and classified them according to their esthetic preference. RESULTS: The result of the t test for independent samples showed differences among states for certain facial profiles. The female photograph identified with the letter 'G' (mandibular retrusion) received higher scores in RS state (p = 0.006). No differences were found for male photographs (p > 0.007). The evaluators' sex seemed not to influence their esthetic perception (p > 0.007). Considering all evaluators together, ANOVA/Tukey's test showed differences among the profiles (p ≤ 0.05) for both male and female photographs. The female photograph that received the highest score was the one identified with the letter 'F' (dentoalveolar bimaxillary retrusion/ straight profile). For the male profiles, photograph identified with the letter 'E' (dentoalveolar bimaxillary protrusion/ straight profile) received the best score. CONCLUSION: Regional differences were observed regarding preferences of facial profile esthetics. In Rio de Janeiro state, more prominent lips were preferred while in Rio Grande do Sul state, profiles with straight lips were favored. Class III profiles were considered less attractive. PMID:26154461

Characterization of Disulfide-Linked Peptides Using Tandem Mass Spectrometry Coupled with Automated Data Analysis Software

NASA Astrophysics Data System (ADS)

Liang, Zhidan; McGuinness, Kenneth N.; Crespo, Alejandro; Zhong, Wendy

2018-05-01

Disulfide bond formation is critical for maintaining structure stability and function of many peptides and proteins. Mass spectrometry has become an important tool for the elucidation of molecular connectivity. However, the interpretation of the tandem mass spectral data of disulfide-linked peptides has been a major challenge due to the lack of appropriate tools. Developing proper data analysis software is essential to quickly characterize disulfide-linked peptides. A thorough and in-depth understanding of how disulfide-linked peptides fragment in mass spectrometer is a key in developing software to interpret the tandem mass spectra of these peptides. Two model peptides with inter- and intra-chain disulfide linkages were used to study fragmentation behavior in both collisional-activated dissociation (CAD) and electron-based dissociation (ExD) experiments. Fragments generated from CAD and ExD can be categorized into three major types, which result from different S-S and C-S bond cleavage patterns. DiSulFinder is a computer algorithm that was newly developed based on the fragmentation observed in these peptides. The software is vendor neutral and capable of quickly and accurately identifying a variety of fragments generated from disulfide-linked peptides. DiSulFinder identifies peptide backbone fragments with S-S and C-S bond cleavages and, more importantly, can also identify fragments with the S-S bond still intact to aid disulfide linkage determination. With the assistance of this software, more comprehensive disulfide connectivity characterization can be achieved. [Figure not available: see fulltext.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guo, Xiaofeng; Xu, Hongwu

Polyhalite is an important coexisting mineral with halite in salt repositories for nuclear waste disposal, such as Waste Isolation Pilot Plant (WIPP) in Carlsbad, New Mexico. The thermal stability of this mineral is a key knowledge in evaluating the integrity of a salt repository in the long term, as water may release due to thermal decomposition of polyhalite. Previous studies on structural evolution of polyhalite at elevated temperatures laid the basis for detailed calorimetric measurements. Using high-temperature oxide-melt drop-solution calorimetry at 975 K with sodium molybdate as the solvent, we have determined the standard enthalpies of formation from constituent sulfatesmore » (ΔH° f,sul), oxides (ΔH° f,ox) and elements (ΔH° f,ele) of a polyhalite sample with the composition of K 2Ca 2Mg(SO 4) 4·1.95H 2O from the Salado formation at the WIPP site. The obtained results are: ΔH° f,sul = -152.5 ± 5.3 kJ/mol, ΔH° f,ox = -1926.1 ± 10.5 kJ/mol, and ΔH° f,ele = -6301.2 ± 9.9 kJ/mol. Furthermore, based on the estimated formation entropies of polyhalite, its standard Gibbs free energy of formation has been derived to be in the range of -5715.3 ± 9.9 kJ/mol to -5739.3 ± 9.9 kJ/mol. In conclusion, these determined thermodynamic properties provide fundamental parameters for modeling the stability behavior of polyhalite in salt repositories.« less

Ground-dwelling ant fauna of sites with high levels of copper.

PubMed

Diehl, E; Sanhudo, C E; Diehl-Fleig, Ed

2004-02-01

Richness and diversity of ant species are related to environmental factors such as vegetation, soil, presence of heavy metals, and insecticides, which allow the use of the assemblage members as terrestrial indicators of environmental conservation status. This study presents the results of ground ants surveyed in Minas do Camaquã in the municipality of Cacapava do Sul (Camaquã Basin), State of Rio Grande do Sul. Collections were performed in four sites, which high levels of copper in the soil, three of which--a mine, a liquid reject, and a solid reject-, had sparse or no plant cover, and one site where Pinus has been used for rehabilitation. Parque das Guaritas was the control site, since it presented normal levels of copper and a dense savanna cover. For each site, three transect lines extending 100 m were draw, and at each 10 m sardine baits were distributed; after two hours the ants present were collected. Hand collections in all five sites were performed during one hour (capture effort). A total of 51 species belonging to 17 genera were collected. The control site was the richest in ant species (r = 45). Sites with high level of copper and poor plant cover presented the lowest richness: mine (r = 14), solid reject (r = 15), and liquid reject (r = 16). In contrast, the site planted with Pinus presented an increment in richness (r = 24) of ground-dwelling ants, suggesting a reahabilitation process.

Cytogenetic and Molecular Data Support the Occurrence of Three Gymnotus Species (Gymnotiformes: Gymnotidae) Used as Live Bait in Corumbá, Brazil: Implications for Conservation and Management of Professional Fishing.

PubMed

de Sousa, Tatiane Pires; Marques, Débora Karla Silvestre; Vitorino, Carla de Andrade; Faria, Karina de Cassia; Braga, Gisele da Silva Ferreira; Ferreira, Daniela Cristina; Venere, Paulo Cesar

2017-04-01

In the Pantanal of Mato Grosso do Sul, electric fish (Gymnotus spp.) are the primary source of live bait, accounting for more than three-quarters of total sales. Based on chromosomal and molecular markers, the present study attempted to identify the Gymnotus species used as bait in the region of Corumbá, Mato Grosso do Sul, Brazil. Three species were detected, based on their distinct karyotypes: G. paraguensis (2n = 54), G. sylvius (2n = 40), and G. pantanal (2n = 39-40, X 1 X 2 Y/X 1 X 1 X 2 X 2 ), with no evidence being found of interspecific hybrids. All three species presented a single nucleolar organizer regions (NOR) (heterochromatin CMA 3 + /DAPI - ) and pericentromeric heterochromatin in almost all chromosomes, with a few distal and/or interstitial blocks. G. sylvius and G. pantanal had one and two pairs of chromosomes with 5S rDNA sites, respectively, while G. paraguensis had 17 chromosome pairs with these markers. The three species formed well-defined clusters in the DNA barcoding analysis. The integrated analysis of the cytogenetic and DNA barcoding data confirmed that the diversity of Gymnotus species exploited as live bait in the study region has been underestimated. These findings indicate that the markers analyzed represent valuable tools for the conservation and fishery management of the Gymnotus stocks exploited.

Transmission of yellow fever vaccine virus through breast-feeding - Brazil, 2009.

PubMed

2010-02-12

In April, 2009, the state health department of Rio Grande do Sul, Brazil, was notified by the Cachoeira do Sul municipal health department of a case of meningoencephalitis requiring hospitalization in an infant whose mother recently had received yellow fever vaccine during a postpartum visit. The Field Epidemiology Training Program of the Secretariat of Surveillance in Health of the Brazilian Ministry of Health assisted state and municipal health departments with an investigation. This report summarizes the results of that investigation, which determined that the infant acquired yellow fever vaccine virus through breast-feeding. The mother reported 2 days of headache, malaise, and low fever occurring 5 days after receipt of yellow fever vaccine. The infant, who was exclusively breast-fed, was hospitalized at age 23 days with seizures requiring continuous infusion of intravenous anticonvulsants. The infant received antimicrobial and antiviral treatment for meningoencephalitis. The presence of 17DD yellow fever virus was detected by reverse transcription--polymerase chain reaction (RT-PCR) in the infant's cerebrospinal fluid (CSF); yellow fever--specific immunoglobulin M (IgM) antibodies also were present in serum and CSF. The infant recovered completely, was discharged after 24 days of hospitalization, and has had normal neurodevelopment and growth through age 6 months. The findings in this report provide documentation that yellow fever vaccine virus can be transmitted via breast-feeding. Administration of yellow fever vaccine to breast-feeding women should be avoided except in situations where exposure to yellow fever viruses cannot be avoided or postponed.

Air-drying beds reduce the quantities of antibiotic resistance genes and class 1 integrons in residual municipal wastewater solids.

PubMed

Burch, Tucker R; Sadowsky, Michael J; LaPara, Timothy M

2013-09-03

This study investigated whether air-drying beds reduce antibiotic resistance gene (ARG) concentrations in residual municipal wastewater solids. Three laboratory-scale drying beds were operated for a period of nearly 100 days. Real-time PCR was used to quantify 16S rRNA genes, 16S rRNA genes specific to fecal bacteria (AllBac) and human fecal bacteria (HF183), the integrase gene of class 1 integrons (intI1), and five ARGs representing a cross-section of antibiotic classes and resistance mechanisms (erm(B), sul1, tet(A), tet(W), and tet(X)). Air-drying beds were capable of reducing all gene target concentrations by 1 to 5 orders of magnitude, and the nature of this reduction was consistent with both a net decrease in the number of bacterial cells and a lack of selection within the microbial community. Half-lives varied between 1.5 d (HF183) and 5.4 d (tet(X)) during the first 20 d of treatment. After the first 20 d of treatment, however, half-lives varied between 8.6 d (tet(X)) and 19.3 d (AllBac), and 16S rRNA gene, intI1, and sul1 concentrations did not change (P > 0.05). These results demonstrate that air-drying beds can reduce ARG and intI1 concentrations in residual municipal wastewater solids within timeframes typical of operating practices.

Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou, China.

PubMed

Chen, Hong; Zhang, Mingmei

2013-08-06

This study aimed at quantifying the concentration and removal of antibiotic resistance genes (ARGs) in three municipal wastewater treatment plants (WWTPs) employing different advanced treatment systems [biological aerated filter, constructed wetland, and ultraviolet (UV) disinfection]. The concentrations of tetM, tetO, tetQ, tetW, sulI, sulII, intI1, and 16S rDNA genes were examined in wastewater and biosolid samples. In municipal WWTPs, ARG reductions of 1-3 orders of magnitude were observed, and no difference was found among the three municipal WWTPs with different treatment processes (p > 0.05). In advanced treatment systems, 1-3 orders of magnitude of reductions in ARGs were observed in constructed wetlands, 0.6-1.2 orders of magnitude of reductions in ARGs were observed in the biological aerated filter, but no apparent decrease by UV disinfection was observed. A significant difference was found between constructed wetlands and biological filter (p < 0.05) and between constructed wetlands and UV disinfection (p < 0.05). In the constructed wetlands, significant correlations were observed in the removal of ARGs and 16S rDNA genes (R(2) = 0.391-0.866; p < 0.05). Constructed wetlands not only have the comparable ARG removal values with WWTP (p > 0.05) but also have the advantage in ARG relative abundance removal, and it should be given priority to be an advanced treatment system for further ARG attenuation from WWTP.

Unchanging pattern of prevalence of esophageal cancer, overall and by histological subtype, in the endoscopy service of the main referral hospital in the central region of Rio Grande do Sul State, in Southern Brazil.

PubMed

Fagundes, R B; de Carli, D; Xaubet, R V; Cantarelli, J C

2016-08-01

Squamous cell carcinoma (SCC) and adenocarcinoma (ADC) are the two main histological types of esophageal cancer. Southern Brazil has the highest rates of esophageal cancer in South America, and the most prevalent subtype of esophageal cancer has been SCC. This study assessed the trend changes in the histological types of esophageal cancer, in a 20-year period, in the central region of Rio Grande do Sul State, Brazil. We searched all cases of esophageal cancer from 1993 to 2012 by their histological diagnosis, grouping the patients in 4-year time periods to evaluate time trends. Among 18 441 upper gastrointestinal endoscopies we identified 686 cases of esophageal cancer. Histological study confirmed the diagnosis of SCC in 640 (93.3%) patients and ADC in 46 (6.7%). Overall, 522 men were diagnosed with esophageal carcinoma; from these, 489 (93.6%) presented SCC, and 33 (6.3%) ADC. Among women, 164 had the diagnosis of esophageal cancer, 151 (92%) SCC, and 13 (7.9%) ADC. The proportion found among men and women was 3.1:1, respectively. The prevalence rate of esophageal cancer, along a 20 year-period, remained stable, as well as the rates of SCC and ADC. SCC was the most common type of esophageal cancer, and ADC presented very low prevalence. © 2015 International Society for Diseases of the Esophagus.

Seroprevalence of Brucella ovis in rams and associated flock level risk factors in the state of Rio Grande do Sul, Brazil.

PubMed

Machado, G; Santos, D V; Kohek, I; Stein, M C; Hein, H E; Poeta, A S; Vidor, A C M; Corbellini, L G

2015-09-01

A cross-sectional study based on a planned probabilistic sampling was carried out to estimate animal and flock prevalence of Brucella ovis in rams, as well as to determine risk factors at the flock level. Data regarding the flocks were collected by means of a questionnaire applied on 705 farms in the state of Rio Grande do Sul, Brazil, using one-stage cluster sampling. From the 705 flocks, 20 (2.5%, CI95%: 2.0-3.1%) had at least one positive ram. At the animal level, out of 1800 rams, 52 were positive (2.89%, CI95%: 0.4-5.3%). Statistical analysis identified the following as risk factors: average age of rams in the flocks (PR: 1.99, CI95%: 1.19-3.32); farms larger than 5 km(2) (500 ha) on extension area (PR: 7.46CI95%: 2.03-27.43); and the lack of lambing paddocks (PR: 5.56, CI95%: 1.70-18.11). This study provided relevant information for authorities to elaborate plans for the first Brazilian state based B. ovis disease control and eradication program. To the authors' knowledge, this is the first study that shows the importance of lambing paddocks in order to keep pre-lambing and lambing ewes away from the rest of the flock, the lack of this infrastructure was considered an important risk factor for B. ovis. Copyright © 2015 Elsevier B.V. All rights reserved.

Identification of foot and mouth disease risk areas using a multi-criteria analysis approach

PubMed Central

Silva, Gustavo Sousa e; Weber, Eliseu José; Hasenack, Heinrich; Groff, Fernando Henrique Sautter; Todeschini, Bernardo; Borba, Mauro Riegert; Medeiros, Antonio Augusto Rosa; Leotti, Vanessa Bielefeldt; Canal, Cláudio Wageck; Corbellini, Luis Gustavo

2017-01-01

Foot and mouth disease (FMD) is a highly infectious disease that affects cloven-hoofed livestock and wildlife. FMD has been a problem for decades, which has led to various measures to control, eradicate and prevent FMD by National Veterinary Services worldwide. Currently, the identification of areas that are at risk of FMD virus incursion and spread is a priority for FMD target surveillance after FMD is eradicated from a given country or region. In our study, a knowledge-driven spatial model was built to identify risk areas for FMD occurrence and to evaluate FMD surveillance performance in Rio Grande do Sul state, Brazil. For this purpose, multi-criteria decision analysis was used as a tool to seek multiple and conflicting criteria to determine a preferred course of action. Thirteen South American experts analyzed 18 variables associated with FMD introduction and dissemination pathways in Rio Grande do Sul. As a result, FMD higher risk areas were identified at international borders and in the central region of the state. The final model was expressed as a raster surface. The predictive ability of the model assessed by comparing, for each cell of the raster surface, the computed model risk scores with a binary variable representing the presence or absence of an FMD outbreak in that cell during the period 1985 to 2015. Current FMD surveillance performance was assessed, and recommendations were made to improve surveillance activities in critical areas. PMID:28552973

Clonality and antimicrobial resistance gene profiles of multidrug- resistant Salmonella enterica serovar infantis isolates from four public hospitals in Rio de Janeiro, Brazil.

PubMed

Fonseca, E L; Mykytczuk, O L; Asensi, M D; Reis, E M F; Ferraz, L R; Paula, F L; Ng, L K; Rodrigues, D P

2006-08-01

In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de Janeiro, Brazil, hospitals between 1996 and 2001, were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing in order to determine their genetic relatedness and antimicrobial resistance profiles. Thirty-four serovar Infantis strains were resistant to at least two antibiotic classes, and all 35 strains were susceptible to fluoroquinolones, cephamycin, and carbapenem. Extended-spectrum beta-lactamase (ESBL) screening by double-disk diffusion indicated that 32 serovar Infantis strains (91.4%) produced beta-lactamases that were inhibited by clavulanic acid. Antimicrobial resistance gene profiles were determined by PCR for a subset of 11 multidrug-resistant serovar Infantis strains, and putative ESBLs were detected by isoelectric focusing. Ten serovar Infantis strains carried bla(TEM), catI, ant(3")Ia and/or ant(3")Ib, sulI and/or sulII, and tet(D) genes as well as an integron-associated aac(6')-Iq cassette. Eight strains possessed at least four different beta-lactamases with pI profiles that confirmed the presence of both ESBLs and non-ESBLs. Our PFGE profiles indicated that 33 serovar Infantis strains isolated from Rio de Janeiro hospitals came from the same genetic lineage.

Characterization of Disulfide-Linked Peptides Using Tandem Mass Spectrometry Coupled with Automated Data Analysis Software

NASA Astrophysics Data System (ADS)

Liang, Zhidan; McGuinness, Kenneth N.; Crespo, Alejandro; Zhong, Wendy

2018-01-01

Disulfide bond formation is critical for maintaining structure stability and function of many peptides and proteins. Mass spectrometry has become an important tool for the elucidation of molecular connectivity. However, the interpretation of the tandem mass spectral data of disulfide-linked peptides has been a major challenge due to the lack of appropriate tools. Developing proper data analysis software is essential to quickly characterize disulfide-linked peptides. A thorough and in-depth understanding of how disulfide-linked peptides fragment in mass spectrometer is a key in developing software to interpret the tandem mass spectra of these peptides. Two model peptides with inter- and intra-chain disulfide linkages were used to study fragmentation behavior in both collisional-activated dissociation (CAD) and electron-based dissociation (ExD) experiments. Fragments generated from CAD and ExD can be categorized into three major types, which result from different S-S and C-S bond cleavage patterns. DiSulFinder is a computer algorithm that was newly developed based on the fragmentation observed in these peptides. The software is vendor neutral and capable of quickly and accurately identifying a variety of fragments generated from disulfide-linked peptides. DiSulFinder identifies peptide backbone fragments with S-S and C-S bond cleavages and, more importantly, can also identify fragments with the S-S bond still intact to aid disulfide linkage determination. With the assistance of this software, more comprehensive disulfide connectivity characterization can be achieved. [Figure not available: see fulltext.

First parasitological, histopathological and molecular characterization of Echinococcus vogeli Rausch and Bernstein, 1972 from Cuniculus paca Linnaeus, 1766 in the Cerrado biome (Mato Grosso do Sul, Brazil).

PubMed

Bittencourt-Oliveira, Fernanda; Teixeira, Paulo; Alencar, Alba; Menezes, Rodrigo; Corrêa, Christiane; Neves, Leandro; Almeida, Fernanda; Daipert-Garcia, Daniel; Machado-Silva, José Roberto; Rodrigues-Silva, Rosângela

2018-01-30

Polycystic echinococcosis (PE) is caused by Echinococcus vogeli metacestodes (larval stage) in Neotropical countries. E. vogeli is trophically-transmitted between predators bush dogs (Speothos venaticus) and prey pacas (Cuniculus paca). In Brazil, reported PE cases are restricted to the Amazon biome. In this study, metacestodes from a paca hunted in Mato Grosso do Sul state (Cerrado biome) were identified morphological and histopathological techniques and further confirmed by molecular testing (sequencing of cytochrome C oxidase subunit I (cox1) gene) for the first time. Images of the whole liver showed superficial bubble-like hepatic masses. The parasitological analysis revealed large hooks (41.3 ± 1.2 μm length/12.8 ± 0.8 μm width) and small hooks (33.0 ± 1.5 μm length/11.1 ± 1.2 μm width), consistent with E. vogeli. Microscopically, the liver showed protoscoleces, a thick laminated layer, fibrosis, and inflammatory infiltrate in the adventitial layer. The DNA sequencing confirmed E. vogeli with 99% homology with sequences deposited in the GenBank. In addition, this finding greatly extends the geographic range of animal polycystic echinococcosis into the Cerrado. It is likely to occur in new biomes, where bush dogs and pacas share a given area in a trophic relationship. Copyright © 2017 Elsevier B.V. All rights reserved.

Variable effects of oxytetracycline on antibiotic resistance gene abundance and the bacterial community during aerobic composting of cow manure.

PubMed

Qian, Xun; Sun, Wei; Gu, Jie; Wang, Xiao-Juan; Sun, Jia-Jun; Yin, Ya-Nan; Duan, Man-Li

2016-09-05

Livestock manure is often subjected to aerobic composting but little is known about the variation in antibiotic resistance genes (ARGs) during the composting process under different concentrations of antibiotics. This study compared the effects of three concentrations of oxytetracycline (OTC; 10, 60, and 200mg/kg) on ARGs and the succession of the bacterial community during composting. Very similar trends were observed in the relative abundances (RAs) of each ARG among the OTC treatments and the control during composting. After composting, the RAs of tetC, tetX, sul1, sul2, and intI1 increased 2-43 times, whereas those of tetQ, tetM, and tetW declined by 44-99%. OTC addition significantly increased the absolute abundances and RAs of tetC and intI1, while 200mg/kg OTC also enhanced those of tetM, tetQ, and drfA7. The bacterial community could be grouped according to the composting time under different treatments. The highest concentration of OTC had a more persistent effect on the bacterial community. In the present study, the succession of the bacterial community appeared to have a greater influence on the variation of ARGs during composting than the presence of antibiotics. Aerobic composting was not effective in reducing most of the ARGs, and thus the compost product should be considered as an important reservoir for ARGs. Copyright © 2016 Elsevier B.V. All rights reserved.

Biophysical parameters in a wheat producer region in southern Brazil

NASA Astrophysics Data System (ADS)

Leivas, Janice F.; de C. Teixeira, Antonio Heriberto; Andrade, Ricardo G.; de C. Victoria, Daniel; Bolfe, Edson L.; Cruz, Caroline R.

2014-10-01

Wheat (Triticum aestivum) is the second most produced cereal in the world, and has major importance in the global agricultural economy. Brazil is a large producer of wheat, especially the Rio Grande do Sul state, located in the south of the country. The purpose of this study was to analyze the estimation of biophysical parameters - evapotranspiration (ET), biomass (BIO) and water productivity (WP) - from satellite images of the municipalities with large areas planted with wheat in Rio Grande do Sul (RS). The evapotranspiration rate was obtained using the SAFER Model (Simple Algorithm for Retrieving Evapotranspiration) on MODIS (Moderate Resolution Imaging Spectroradiometer) images taken in the agricultural year 2012. In order to obtain biomass and water productivity rates we applied the Monteith model and the ratio between BIO and ET. In the beginning of the cycle (the planting period) we observed low values for ET, BIO and WP. During the development period, we observed an increase in the values of the parameters and decline at the end of the cycle, for the period of the wheat harvest. The SAFER model proved effective for estimating the biophysical parameters evapotranspiration, biomass production and water productivity in areas planted with wheat in Brazilian Southern. The methodology can be used for monitoring the crops' water conditions and biomass using satellite images, assisting in estimates of productivity and crop yield. The results may assist the understanding of biophysical properties of important agro-ecosystems, like wheat crop, and are important to improve the rational use of water resources.

Toward shrimp consumption without chemicals: Combined effects of freezing and modified atmosphere packaging (MAP) on some quality characteristics of Giant Red Shrimp (Aristaeomorpha foliacea) during storage.

PubMed

Bono, Gioacchino; Okpala, Charles Odilichukwu R; Alberio, Giuseppina R A; Messina, Concetta M; Santulli, Andrea; Giacalone, Gabriele; Spagna, Giovanni

2016-04-15

The combined effects of freezing and modified atmosphere packaging (MAP) (100% N2 and 50% N2+50% CO2) on some quality characteristics of Giant Red Shrimp (GRS) (Aristaeomorpha foliacea) was studied during 12-month storage. In particular, the quality characteristics determined proximal and gas compositions, melanosis scores, pH, total volatile basic-nitrogen (TVB-N), thiobarbituric acid (TBA) as well as free amino acid (FAA). In addition, the emergent data were compared to those subject to vacuum packaging as well as conventional preservative method of sulphite treatment (SUL). Most determined qualities exhibited quantitative differences with storage. By comparisons, while pH and TVB-N statistically varied between treatments (P<0.05) and TBA that ranged between ∼0.15 and 0.30 mg MDA/kg appeared least at end of storage for 100% N2 treated-group, the latter having decreased melanosis scores showed such treatments with high promise to keep the colour of GRS sample hence, potential replacement for SUL group. By comparisons also, while some individual FAA values showed increases especially at the 100% N2-treated group, the total FAAs statistically differed with storage (P<0.05). The combination of freezing and MAP treatments as preservative treatment method shows high promise to influence some quality characteristics of GRS samples of this study. Copyright © 2015 Elsevier Ltd. All rights reserved.

Survival and SOS response induction in ultraviolet B irradiated Escherichia coli cells with defective repair mechanisms.

PubMed

Prada Medina, Cesar Augusto; Aristizabal Tessmer, Elke Tatjana; Quintero Ruiz, Nathalia; Serment-Guerrero, Jorge; Fuentes, Jorge Luis

2016-06-01

Purpose In this paper, the contribution of different genes involved in DNA repair for both survival and SOS induction in Escherichia coli mutants exposed to ultraviolet B radiation (UVB, [wavelength range 280-315 nm]) was evaluated. Materials and methods E. coli strains defective in uvrA, oxyR, recO, recN, recJ, exoX, recB, recD or xonA genes were used to determine cell survival. All strains also had the genetic sulA::lacZ fusion, which allowed for the quantification of SOS induction through the SOS Chromotest. Results Five gene products were particularly important for survival, as follows: UvrA > RecB > RecO > RecJ > XonA. Strains defective in uvrA and recJ genes showed elevated SOS induction compared with the wild type, which remained stable for up to 240 min after UVB-irradiation. In addition, E. coli strains carrying the recO or recN mutation showed no SOS induction. Conclusions The nucleotide excision and DNA recombination pathways were equally used to repair UVB-induced DNA damage in E. coli cells. The sulA gene was not turned off in strains defective in UvrA and RecJ. RecO protein was essential for processing DNA damage prior to SOS induction. In this study, the roles of DNA repair proteins and their contributions to the mechanisms that induce SOS genes in E. coli are proposed.

Factors associated with caries: a survey of students from southern Brazil

PubMed Central

Borges, Tássia Silvana; Schwanke, Natalí Lippert; Reuter, Cézane Priscila; Neto, Léo Kraether; Burgos, Miria Suzana

2016-01-01

Abstract Objective: To describe the factors associated with dental caries among students from Santa Cruz do Sul, Rio Grande do Sul, Brazil. Methods: A cross-sectional study was conducted in a random sample of 623 students of both genders, aged 10-17 years old. Tooth decay was performed using the index of the World Health Organization (1997), DMFT (permanent dentition) that expresses the sum of decayed, missing and filled teeth per person. The maternal educational level was rated using criteria of the Brazilian Association of Market Research Companies. The remaining variables were obtained by a structured questionnaire. Poisson regression analysis was used to test the association between variables using robust models and a subsequently adjusted model. Data were expressed as prevalence ratio (PR). Results: Multivariate analysis identified the following factors related to the experience of dental caries: residence in rural municipalities (PR: 1.15; 95%CI: 1.0-1.3), attending a city school (PR: 3.30; 95%CI: 1.1-9.4) or a state school (PR: 3.40; 95%CI: 1.1-9.6); and having an illiterate mother or a mother that only attended up to the 4th year of school (PR: 1.67; 95%CI: 1.1-2.4) or high school (PR: 1.54; 95%CI: 1.1-2.2). Conclusions: The presence of caries in students in southern Brazil was associated with residence in rural areas, mother with little education and attendance to a public school. PMID:27477791