40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2010 CFR

2010-07-01

... mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2011 CFR

2011-07-01

... mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

40 CFR 405.70 - Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory.

Code of Federal Regulations, 2012 CFR

2012-07-01

... fluid mix for ice cream and other frozen desserts subcategory. 405.70 Section 405.70 Protection of... PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory § 405.70 Applicability; description of the fluid mix for ice cream and other frozen desserts subcategory. The provisions...

The Implement of a Multi-layer Frozen Soil Scheme into SSiB3 and its Evaluation over Cold Regions

NASA Astrophysics Data System (ADS)

Li, Q.

2016-12-01

The SSiB3 is a biophysics-based model of land-atmosphere interactions and is designed for global and regional studies. It has three soil layers, three snow layers, as well as one vegetation layer. Soil moisture of the three soil layers, interception water store for the canopy, subsurface soil temperature, ground temperature, canopy temperature and snow water equivalent are all predicted based on the water and energy balance at canopy, soil and snow. SSiB3 substantially enhances the model's capability for cold season studies and produces reasonable results compared with observations. However, frozen soil processes are ignored in the SSiB3 and may have effects on the interannual variability of soil temperature and deep soil memory. A multi-layer comprehensive frozen soil scheme (FSM), which is developed for climate study has been implemented into the SSiB3 to describe soil heat transfer and water flow affected by frozen processed in soil. In the coupled SSiB3-FSM, both liquid water and ice content have been taken into account in the frozen soil hydrologic and thermal property parameterization. The maximum soil layer depth could reach 10 meters thick depending on land conditions. To better evaluate the models' performance, the coupled offline SSiB3-FSM and SSiB3 have been driven from 1948 to 1958 by the Princeton global meteorological data set, respectively. For the 10yrs run, the coupled SSiB3-FSM almost captures the features over different regions, especially cold regions. In order to analysis and compare the differences of SSIB3-FSM and SSIB3 in detail, monthly mean surface temperature for different regions are compared with CAMS data. The statistical results of surface skin temperature show that high latitude regions, Africa, Eastern Australia, and North American monsoon regions have been greatly improved in SSIB3-FSM. For the global statistics, the RMSE of the surface temperature simulated by SSiB3-FSM can be improved about 0.6K compared to SSiB3. In this study, the improvements in the coupled SSiB3-FSM have also been analyzed.

Evaluation of snow and frozen soil parameterization in a cryosphere land surface modeling framework in the Tibetan Plateau

NASA Astrophysics Data System (ADS)

Zhou, J.

2017-12-01

Snow and frozen soil are important components in the Tibetan Plateau, and influence the water cycle and energy balances through snowpack accumulation and melt and soil freeze-thaw. In this study, a new cryosphere land surface model (LSM) with coupled snow and frozen soil parameterization was developed based on a hydrologically improved LSM (HydroSiB2). First, an energy-balance-based three-layer snow model was incorporated into HydroSiB2 (hereafter HydroSiB2-S) to provide an improved description of the internal processes of the snow pack. Second, a universal and simplified soil model was coupled with HydroSiB2-S to depict soil water freezing and thawing (hereafter HydroSiB2-SF). In order to avoid the instability caused by the uncertainty in estimating water phase changes, enthalpy was adopted as a prognostic variable instead of snow/soil temperature in the energy balance equation of the snow/frozen soil module. The newly developed models were then carefully evaluated at two typical sites of the Tibetan Plateau (TP) (one snow covered and the other snow free, both with underlying frozen soil). At the snow-covered site in northeastern TP (DY), HydroSiB2-SF demonstrated significant improvements over HydroSiB2-F (same as HydroSiB2-SF but using the original single-layer snow module of HydroSiB2), showing the importance of snow internal processes in three-layer snow parameterization. At the snow-free site in southwestern TP (Ngari), HydroSiB2-SF reasonably simulated soil water phase changes while HydroSiB2-S did not, indicating the crucial role of frozen soil parameterization in depicting the soil thermal and water dynamics. Finally, HydroSiB2-SF proved to be capable of simulating upward moisture fluxes toward the freezing front from the underlying soil layers in winter.

Development of a land surface model with coupled snow and frozen soil physics

NASA Astrophysics Data System (ADS)

Wang, Lei; Zhou, Jing; Qi, Jia; Sun, Litao; Yang, Kun; Tian, Lide; Lin, Yanluan; Liu, Wenbin; Shrestha, Maheswor; Xue, Yongkang; Koike, Toshio; Ma, Yaoming; Li, Xiuping; Chen, Yingying; Chen, Deliang; Piao, Shilong; Lu, Hui

2017-06-01

Snow and frozen soil are important factors that influence terrestrial water and energy balances through snowpack accumulation and melt and soil freeze-thaw. In this study, a new land surface model (LSM) with coupled snow and frozen soil physics was developed based on a hydrologically improved LSM (HydroSiB2). First, an energy-balance-based three-layer snow model was incorporated into HydroSiB2 (hereafter HydroSiB2-S) to provide an improved description of the internal processes of the snow pack. Second, a universal and simplified soil model was coupled with HydroSiB2-S to depict soil water freezing and thawing (hereafter HydroSiB2-SF). In order to avoid the instability caused by the uncertainty in estimating water phase changes, enthalpy was adopted as a prognostic variable instead of snow/soil temperature in the energy balance equation of the snow/frozen soil module. The newly developed models were then carefully evaluated at two typical sites of the Tibetan Plateau (TP) (one snow covered and the other snow free, both with underlying frozen soil). At the snow-covered site in northeastern TP (DY), HydroSiB2-SF demonstrated significant improvements over HydroSiB2-F (same as HydroSiB2-SF but using the original single-layer snow module of HydroSiB2), showing the importance of snow internal processes in three-layer snow parameterization. At the snow-free site in southwestern TP (Ngari), HydroSiB2-SF reasonably simulated soil water phase changes while HydroSiB2-S did not, indicating the crucial role of frozen soil parameterization in depicting the soil thermal and water dynamics. Finally, HydroSiB2-SF proved to be capable of simulating upward moisture fluxes toward the freezing front from the underlying soil layers in winter.

Ignition of deuterium-trtium fuel targets

DOEpatents

Musinski, Donald L.; Mruzek, Michael T.

1991-01-01

A method of igniting a deuterium-tritium ICF fuel target to obtain fuel burn in which the fuel target initially includes a hollow spherical shell having a frozen layer of DT material at substantially uniform thickness and cryogenic temperature around the interior surface of the shell. The target is permitted to free-fall through a target chamber having walls heated by successive target ignitions, so that the target is uniformly heated during free-fall to at least partially melt the frozen fuel layer and form a liquid single-phase layer or a mixed liquid/solid bi-phase layer of substantially uniform thickness around the interior shell surface. The falling target is then illuminated from exteriorly of the chamber while the fuel layer is at substantially uniformly single or bi-phase so as to ignite the fuel layer and release energy therefrom.

Ignition of deuterium-tritium fuel targets

DOEpatents

Musinski, D.L.; Mruzek, M.T.

1991-08-27

Disclosed is a method of igniting a deuterium-tritium ICF fuel target to obtain fuel burn in which the fuel target initially includes a hollow spherical shell having a frozen layer of DT material at substantially uniform thickness and cryogenic temperature around the interior surface of the shell. The target is permitted to free-fall through a target chamber having walls heated by successive target ignitions, so that the target is uniformly heated during free-fall to at least partially melt the frozen fuel layer and form a liquid single-phase layer or a mixed liquid/solid bi-phase layer of substantially uniform thickness around the interior shell surface. The falling target is then illuminated from exteriorly of the chamber while the fuel layer is at substantially uniformly single or bi-phase so as to ignite the fuel layer and release energy therefrom. 5 figures.

The Replacement of Monosaccharide by Mannitol or Sorbitol in the Freezing Extender Enhances Cryosurvival of Ram Spermatozoa.

PubMed

Wu, Guo Quan; Lv, Chun Rong; Jiang, Yan Ting; Wang, Si Yu; Shao, Qing Yong; Hong, Qiong Hua; Quan, Guo Bo

2016-10-01

In this study, the protective effects of monosaccharides (glucose and fructose) and sugar alcohols (mannitol, sorbitol, and xylitol) on frozen ram spermatozoa were evaluated and compared. The motility, moving velocity, and hypoosmotic swelling capability of spermatozoa frozen with monosaccharide or sugar alcohol were measured using a computer-assisted spermatozoa analyzer system. The acrosome status, membrane integrity, distribution of phosphatidylserine (PS), and mitochondrial membrane potential (MMP) were analyzed using fluorescence staining and flow cytometry. The results indicated that similar to glucose or fructose, the presence of sugar alcohol in the freezing extender cannot significantly improve the motility and moving velocity of ram spermatozoa equilibrated at 5°C. In terms of motility, pathway velocity, curve velocity, hypoosmotic swelling capability, acrosome and membrane integrity, and MMP, the inclusion of mannitol or sorbitol in the extender can significantly improve the quality of frozen-thawed ram spermatozoa compared to glucose or fructose. However, the effects of mannitol or sorbitol on linear velocity and PS distribution of frozen-thawed spermatozoa were similar to those of the monosaccharides (p > 0.05). In addition, the ability of xylitol to protect acrosome and maintain MMP in frozen-thawed spermatozoa was significantly higher compared with glucose or fructose (p < 0.05), although it could not improve the other evaluated parameters. Finally, there is no significant difference existing between mannitol and sorbitol with respect to the above evaluated parameters. In conclusion, the replacement of glucose or fructose by mannitol or sorbitol in a freezing extender can improve the postthaw quality of ram spermatozoa under specific freezing conditions. Moreover, the protective effects of mannitol and sorbitol on frozen-thawed ram spermatozoa are superior to that of xylitol. However, in the presence of sugar alcohols, the cryoinjury on spermatozoa membrane is still serious. In the future, the question of protecting the membrane of frozen-thawed spermatozoa needs further research.

Comparison of algorithms and parameterisations for infiltration into organic-covered permafrost soils

USDA-ARS?s Scientific Manuscript database

Infiltration into frozen and unfrozen soils is critical in hydrology, controlling active layer soil water dynamics and influencing runoff. Few Land Surface Models (LSMs) and Hydrological Models (HMs) have been developed, adapted or tested for frozen conditions and permafrost soils. Considering the v...

40 CFR 405.80 - Applicability; description of the ice cream, frozen desserts, novelties and other dairy desserts...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Applicability; description of the ice... PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts Subcategory § 405.80 Applicability; description of the ice cream, frozen desserts, novelties and other dairy...

40 CFR 405.80 - Applicability; description of the ice cream, frozen desserts, novelties and other dairy desserts...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Applicability; description of the ice... PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts Subcategory § 405.80 Applicability; description of the ice cream, frozen desserts, novelties and other dairy...

40 CFR 405.80 - Applicability; description of the ice cream, frozen desserts, novelties and other dairy desserts...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Applicability; description of the ice... PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts Subcategory § 405.80 Applicability; description of the ice cream, frozen desserts, novelties and other dairy...

40 CFR 405.80 - Applicability; description of the ice cream, frozen desserts, novelties and other dairy desserts...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Applicability; description of the ice... PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts Subcategory § 405.80 Applicability; description of the ice cream, frozen desserts, novelties and other dairy...

40 CFR 405.80 - Applicability; description of the ice cream, frozen desserts, novelties and other dairy desserts...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Applicability; description of the ice... PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts Subcategory § 405.80 Applicability; description of the ice cream, frozen desserts, novelties and other dairy...

Dissolved organic carbon and nitrogen release from Holocene permafrost and seasonally frozen soils

NASA Astrophysics Data System (ADS)

Wickland, K.; Waldrop, M. P.; Koch, J. C.; Jorgenson, T.; Striegl, R. G.

2017-12-01

Permafrost (perennially frozen) soils store vast amounts of carbon (C) and nitrogen (N) that are vulnerable to mobilization to the atmosphere as greenhouse gases and to terrestrial and aquatic ecosystems as dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) upon thaw. Such releases will affect the biogeochemistry of arctic and boreal regions, yet little is known about active layer (seasonally frozen) and permafrost source variability that determines DOC and TDN mobilization. We quantified DOC and TDN leachate yields from a range of active layer and permafrost soils in Alaska varying in age and C and N content to determine potential release upon thaw. Soil cores from the upper 1 meter were collected in late winter, when soils were frozen, from three locations representing a range in geographic position, landscape setting, permafrost depth, and soil types across interior Alaska. Two 15 cm-thick segments were extracted from each core: a deep active-layer horizon and a shallow permafrost horizon. Soils were thawed and leached for DOC and TDN yields, dissolved organic matter optical properties, and DOC biodegradability; soils were analyzed for C and N content, and radiocarbon content. Soils had wide-ranging C and N content (<1-44% C, <0.1-2.3% N), and varied in radiocarbon age from 450-9200 years before present - thus capturing typical ranges of boreal and arctic soils. Soil DOC and TDN yields increased linearly with soil C and N content, and decreased with increasing radiocarbon age. However, across all sites DOC and TDN yields were significantly greater from permafrost soils (0.387 ± 0.324 mg DOC g-1 soil; 0.271 ± 0.0271 mg N g-1 soil) than from active layer soils (0.210 ± 0.192 mg DOC g-1 soil; 0.00716 ± 0.00569 mg N g-1 soil). DOC biodegradability increased with increasing radiocarbon age, and was statistically similar for active layer and permafrost soils. Our findings suggest that the continuously frozen state of permafrost soils has preserved higher relative potential DOC and TDN yields compared to seasonally thawed soils exposed to annual leaching and decomposition, and that frozen soils undergo microbial processes that produce labile DOC over time.

The protective effect of rapid cold-hardening develops more quickly in frozen versus supercooled larvae of the Antarctic midge, Belgica antarctica.

PubMed

Kawarasaki, Yuta; Teets, Nicholas M; Denlinger, David L; Lee, Richard E

2013-10-15

During the austral summer, larvae of the terrestrial midge Belgica antarctica (Diptera: Chironomidae) experience highly variable and often unpredictable thermal conditions. In addition to remaining freeze tolerant year-round, larvae are capable of swiftly increasing their cold tolerance through the rapid cold-hardening (RCH) response. The present study compared the induction of RCH in frozen versus supercooled larvae. At the same induction temperature, RCH occurred more rapidly and conferred a greater level of cryoprotection in frozen versus supercooled larvae. Furthermore, RCH in frozen larvae could be induced at temperatures as low as -12°C, which is the lowest temperature reported to induce RCH. Remarkably, as little as 15 min at -5°C significantly enhanced larval cold tolerance. Not only is protection from RCH acquired swiftly, but it is also quickly lost after thawing for 2 h at 2°C. Because the primary difference between frozen and supercooled larvae is cellular dehydration caused by freeze concentration of body fluids, we also compared the effects of acclimation in dehydrated versus frozen larvae. Because slow dehydration without chilling significantly increased larval survival to a subsequent cold exposure, we hypothesize that cellular dehydration caused by freeze concentration promotes the rapid acquisition of cold tolerance in frozen larvae.

Active layer hydrology in an arctic tundra ecosystem: quantifying water sources and cycling using water stable isotopes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Throckmorton, Heather M.; Newman, Brent D.; Heikoop, Jeffrey M.

Climate change and thawing permafrost in the Arctic will significantly alter landscape hydro-geomorphology and the distribution of soil moisture, which will have cascading effects on climate feedbacks (CO 2 and CH 4) and plant and microbial communities. Fundamental processes critical to predicting active layer hydrology are not well understood. This study applied water stable isotope techniques (δ 2H and δ 18O) to infer sources and mixing of active layer waters in a polygonal tundra landscape in Barrow, Alaska (USA), in August and September of 2012. Results suggested that winter precipitation did not contribute substantially to surface waters or subsurface activemore » layer pore waters measured in August and September. Summer rain was the main source of water to the active layer, with seasonal ice melt contributing to deeper pore waters later in the season. Surface water evaporation was evident in August from a characteristic isotopic fractionation slope (δ 2H vs δ 18O). Freeze-out isotopic fractionation effects in frozen active layer samples and textural permafrost were indistinguishable from evaporation fractionation, emphasizing the importance of considering the most likely processes in water isotope studies, in systems where both evaporation and freeze-out occur in close proximity. The fractionation observed in frozen active layer ice was not observed in liquid active layer pore waters. Such a discrepancy between frozen and liquid active layer samples suggests mixing of meltwater, likely due to slow melting of seasonal ice. In conclusion, this research provides insight into fundamental processes relating to sources and mixing of active layer waters, which should be considered in process-based fine-scale and intermediate-scale hydrologic models.« less

Active layer hydrology in an arctic tundra ecosystem: quantifying water sources and cycling using water stable isotopes

DOE PAGES

Throckmorton, Heather M.; Newman, Brent D.; Heikoop, Jeffrey M.; ...

2016-04-16

Climate change and thawing permafrost in the Arctic will significantly alter landscape hydro-geomorphology and the distribution of soil moisture, which will have cascading effects on climate feedbacks (CO 2 and CH 4) and plant and microbial communities. Fundamental processes critical to predicting active layer hydrology are not well understood. This study applied water stable isotope techniques (δ 2H and δ 18O) to infer sources and mixing of active layer waters in a polygonal tundra landscape in Barrow, Alaska (USA), in August and September of 2012. Results suggested that winter precipitation did not contribute substantially to surface waters or subsurface activemore » layer pore waters measured in August and September. Summer rain was the main source of water to the active layer, with seasonal ice melt contributing to deeper pore waters later in the season. Surface water evaporation was evident in August from a characteristic isotopic fractionation slope (δ 2H vs δ 18O). Freeze-out isotopic fractionation effects in frozen active layer samples and textural permafrost were indistinguishable from evaporation fractionation, emphasizing the importance of considering the most likely processes in water isotope studies, in systems where both evaporation and freeze-out occur in close proximity. The fractionation observed in frozen active layer ice was not observed in liquid active layer pore waters. Such a discrepancy between frozen and liquid active layer samples suggests mixing of meltwater, likely due to slow melting of seasonal ice. In conclusion, this research provides insight into fundamental processes relating to sources and mixing of active layer waters, which should be considered in process-based fine-scale and intermediate-scale hydrologic models.« less

Heat stress control in the TMI-2 (Three Mile Island Unit 2) defueling and decontamination activities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schork, J.S.; Parfitt, B.A.

During the initial stages of the Three Mile Island Unit 2 (TMI-2) defueling and decontamination activities for the reactor building, it was realized that the high levels of loose radioactive contamination would require the use of extensive protective clothing by entry personnel. While there was no doubt that layered protective clothing protects workers from becoming contaminated, it was recognized that these same layers of clothing would impose a very significant heat stress burden. To prevent the potentially serious consequences of a severe reaction to heat stress by workers in the hostile environment of the TMI-2 reactor building and yet maintainmore » the reasonable work productivity necessary to perform the recovery adequately, an effective program of controlling worker exposure to heat stress had to be developed. Body-cooling devices produce a flow of cool air, which is introduced close to the skin to remove body heat through convection and increased sweat evaporation. The cooling effect produced by the Vortex tube successfully protected the workers from heat stress, however, there were several logistical and operational problems that hindered extensive use of these devices. The last type of cooling garment examined was the frozen water garment (FWG) developed by Elizier Kamon at the Pennsylvania State University as part of an Electric Power Research Institute research grant. Personal protection, i.e., body cooling, engineering controls, and administrative controls, have been implemented successfully.« less

Dynamic behavior of an unsteady trubulent boundary layer

NASA Technical Reports Server (NTRS)

Parikh, P. G.; Reynolds, W. C.; Jayaramen, R.; Carr, L. W.

1981-01-01

Experiments on an unsteady turbulent boundary layer are reported in which the upstream portion of the flow is steady (in the mean) and in the downstream region, the boundary layer sees a linearly decreasing free stream velocity. This velocity gradient oscillates in time, at frequencies ranging from zero to approximately the bursting frequency. For the small amplitude, the mean velocity and mean turbulence intensity profiles are unaffected by the oscillations. The amplitude of the periodic velocity component, although as much as 70% greater than that in the free stream for very low frequencies, becomes equal to that in the free stream at higher frequencies. At high frequencies, both the boundary layer thickness and the Reynolds stress distribution across the boundary layer become frozen. The behavior at higher amplitude is quite similar. At sufficiently high frequencies, the boundary layer thickness remains frozen at the mean value over the oscillation cycle, even though flow reverses near the wall during a part of the cycle.

Using the Cn2 and wind profiler method with wide-field laser-guide-stars adaptive optics to quantify the frozen-flow decay

NASA Astrophysics Data System (ADS)

Guesalaga, Andrés; Neichel, Benoit; Cortés, Angela; Béchet, Clémentine; Guzmán, Dani

2014-05-01

We use the spatio-temporal cross-correlations of slopes from five Shack-Hartmann wavefront sensors to analyse the temporal evolution of the atmospheric turbulence layers at different altitudes. The focus is on the verification of the frozen-flow assumption. The data come from the Gemini South Multiconjugate Adaptive Optics System (GeMS). First, we present the Cn2 and wind profiling technique. This method provides useful information for the operation of the adaptive optics system, such as the number of existing turbulence layers, their associated velocities, altitudes and strengths, and also a mechanism to estimate the dome-seeing contribution to the total turbulence. Next, by identifying the turbulence layers, we show that it is possible to estimate the rate of decay in time of the correlation among turbulence measurements. We reduce on-sky data obtained during the 2011, 2012 and 2013 campaigns. The first results suggest that the rate of temporal decorrelation can be expressed in terms of a single parameter that is independent of the layer altitude and turbulence strength. Finally, we show that the decay rate of the frozen-flow contribution increases linearly with the layer speed. The observed evolution of the decay rate confirms the potential interest of the predictive control for wide-field adaptive optics systems.

Monodisperse, polymeric microspheres produced by irradiation of slowly thawing frozen drops

NASA Technical Reports Server (NTRS)

Rhim, Won-Kyu (Inventor); Hyson, Michael T. (Inventor); Chung, Sang-Kun (Inventor); Colvin, Michael S. (Inventor); Chang, Manchium (Inventor)

1991-01-01

Monodisperse, polymeric microspheres are formed by injecting uniformly shaped droplets of radiation polymerizable monomers, preferably a biocompatible monomer, having covalent binding sites such as hydroxyethylmethacrylate, into a zone, impressing a like charge on the droplet so that they mutually repel each other, spheroidizing the droplets within the zone and collecting the droplets in a pool of cryogenic liquid. As the droplets enter the liquid, they freeze into solid, glassy microspheres, which vaporizes a portion of the cryogenic liquid to form a layer. The like-charged microspheres, suspended within the layer, move to the edge of the vessel holding the pool, are discharged, fall and are collected. The collected microspheres are irradiated while frozen in the cryogenic liquid to form latent free radicals. The frozen microspheres are then slowly thawed to activate the free radicals which polymerize the monomer to form evenly-sized, evenly-shaped, monodisperse polymeric microspheres.

Oscillatory interfacial instability between miscible fluids

NASA Astrophysics Data System (ADS)

Shevtsova, Valentina; Gaponenko, Yuri; Mialdun, Aliaksandr; Torregrosa, Marita; Yasnou, Viktar

Interfacial instabilities occurring between two fluids are of fundamental interest in fluid dynamics, biological systems and engineering applications such as liquid storage, solvent extraction, oil recovery and mixing. Horizontal vibrations applied to stratified layers of immiscible liquids may generate spatially periodic waving of the interface, stationary in the reference frame of the vibrated cell, referred to as a "frozen wave". We present experimental evidence that frozen wave instability exists between two ordinary miscible liquids of similar densities and viscosities. At the experiments and at the numerical model, two superimposed layers of ordinary liquids, water-alcohol of different concentrations, are placed in a closed cavity in a gravitationally stable configuration. The density and viscosity of these fluids are somewhat similar. Similar to the immiscible fluids this instability has a threshold. When the value of forcing is increased the amplitudes of perturbations grow continuously displaying a saw-tooth structure. The decrease of gravity drastically changes the structure of frozen waves.

Dissolved organic carbon and nitrogen release from boreal Holocene permafrost and seasonally frozen soils of Alaska

NASA Astrophysics Data System (ADS)

Wickland, Kimberly P.; Waldrop, Mark P.; Aiken, George R.; Koch, Joshua C.; Torre Jorgenson, M.; Striegl, Robert G.

2018-06-01

Permafrost (perennially frozen) soils store vast amounts of organic carbon (C) and nitrogen (N) that are vulnerable to mobilization as dissolved organic carbon (DOC) and dissolved organic and inorganic nitrogen (DON, DIN) upon thaw. Such releases will affect the biogeochemistry of permafrost regions, yet little is known about the chemical composition and source variability of active-layer (seasonally frozen) and permafrost soil DOC, DON and DIN. We quantified DOC, total dissolved N (TDN), DON, and DIN leachate yields from deep active-layer and near-surface boreal Holocene permafrost soils in interior Alaska varying in soil C and N content and radiocarbon age to determine potential release upon thaw. Soil cores were collected at three sites distributed across the Alaska boreal region in late winter, cut in 15 cm thick sections, and deep active-layer and shallow permafrost sections were thawed and leached. Leachates were analyzed for DOC, TDN, nitrate (NO3 ‑), and ammonium (NH4 +) concentrations, dissolved organic matter optical properties, and DOC biodegradability. Soils were analyzed for C, N, and radiocarbon (14C) content. Soil DOC, TDN, DON, and DIN yields increased linearly with soil C and N content, and decreased with increasing radiocarbon age. These relationships were significantly different for active-layer and permafrost soils such that for a given soil C or N content, or radiocarbon age, permafrost soils released more DOC and TDN (mostly as DON) per gram soil than active-layer soils. Permafrost soil DOC biodegradability was significantly correlated with soil Δ14C and DOM optical properties. Our results demonstrate that near-surface Holocene permafrost soils preserve greater relative potential DOC and TDN yields than overlying seasonally frozen soils that are exposed to annual leaching and decomposition. While many factors control the fate of DOC and TDN, the greater relative yields from newly thawed Holocene permafrost soils will have the largest potential impact in areas dominated by organic-rich soils.

Significance of vapor phase chemical reactions on CVD rates predicted by chemically frozen and local thermochemical equilibrium boundary layer theories

NASA Technical Reports Server (NTRS)

Gokoglu, Suleyman A.

1988-01-01

This paper investigates the role played by vapor-phase chemical reactions on CVD rates by comparing the results of two extreme theories developed to predict CVD mass transport rates in the absence of interfacial kinetic barrier: one based on chemically frozen boundary layer and the other based on local thermochemical equilibrium. Both theories consider laminar convective-diffusion boundary layers at high Reynolds numbers and include thermal (Soret) diffusion and variable property effects. As an example, Na2SO4 deposition was studied. It was found that gas phase reactions have no important role on Na2SO4 deposition rates and on the predictions of the theories. The implications of the predictions of the two theories to other CVD systems are discussed.

High enthalpy hypersonic boundary layer flow

NASA Technical Reports Server (NTRS)

Yanow, G.

1972-01-01

A theoretical and experimental study of an ionizing laminar boundary layer formed by a very high enthalpy flow (in excess of 12 eV per atom or 7000 cal/gm) with allowance for the presence of helium driver gas is described. The theoretical investigation has shown that the use of variable transport properties and their respective derivatives is very important in the solution of equilibrium boundary layer equations of high enthalpy flow. The effect of low level helium contamination on the surface heat transfer rate is minimal. The variation of ionization is much smaller in a chemically frozen boundary layer solution than in an equilibrium boundary layer calculation and consequently, the variation of the transport properties in the case of the former was not essential in the integration. The experiments have been conducted in a free piston shock tunnel, and a detailed study of its nozzle operation, including the effects of low levels of helium driver gas contamination has been made. Neither the extreme solutions of an equilibrium nor of a frozen boundary layer will adequately predict surface heat transfer rate in very high enthalpy flows.

How will the future warming climate impact the river discharge in the alpine mountain region of upper Heihe River Basin?

NASA Astrophysics Data System (ADS)

Wang, Y.; Yang, H.; Yang, D.; Gao, B.; Qin, Y.

2017-12-01

The Tibetan Plateau is more sensitive to the global climate change than other areas due to its special geography. Previous studies have shown that, besides the changes of temperature and precipitation, the changes in the cryosphere such as glacier and frozen ground also have important and far-reaching effects on the ecological and hydrological processes in the basin. In order to reliably predict the future runoff changing trend in the future, it's important to estimate the responses of cryosphere to the future climate change, as well as its impacts on the hydrological processes. Based on typical future climate scenarios (under emission scenario RCP4.5) from five general circulation models (GCMs) and one regional climate model (RCM), as well as a distributed eco-hydrological model (GBEHM), this study analyzes the possible future climate change (from 2011 to 2060) and its impacts on cryospheric and hydrological processes in upper Heihe River Basin, a typical cold mountain region located in the Northeast Tibetan Plateau. The results suggest that air temperature is expected to rise in the future by approximately 0.32 °C/10a, and precipitation is expected to rise slightly by about 3 mm/10a. Under the rising air temperature, the maximum frozen depth of seasonally frozen ground will decrease by about 4.1 cm/10a and the active layer depth of the frozen ground will increase by about 6.2 cm/10a. The runoff is expected to reduce by approximately 6 mm/10a and the evapotranspiration is expected to increase by approximately 9 mm/10a. These changes in hydrological processes are mainly caused by the air temperature rise. The impacts of air temperature change on the hydrological processes are mainly due to the changes of frozen ground. The thickening of active layer of the frozen ground increases the soil storage capacity, leading to the decrease of runoff and increase of evapotranspiration. Results show that, when the active layer depth increase by 1 cm, the runoff will decrease by about 1 2 mm and the evapotranspiration will increase by about 0.7 2 mm. Additionally, the changes from permafrost to seasonal frozen ground increase the groundwater infiltration, which also leads to the decrease of surface runoff.

Resilient modulus characterization of Alaskan granular base materials.

DOT National Transportation Integrated Search

2010-08-01

When spring comes to cold regions, the active layer (the top few feet of soil that freezes and thaws seasonally) thaws quickly, while : deeper soil remains frozen. The active layer becomes saturated with water from snowmelt that collects atop the fro...

Microphysical properties of frozen particles inferred from Global Precipitation Measurement (GPM) Microwave Imager (GMI) polarimetric measurements

NASA Astrophysics Data System (ADS)

Gong, Jie; Wu, Dong L.

2017-02-01

Scattering differences induced by frozen particle microphysical properties are investigated, using the vertically (V) and horizontally (H) polarized radiances from the Global Precipitation Measurement (GPM) Microwave Imager (GMI) 89 and 166 GHz channels. It is the first study on frozen particle microphysical properties on a global scale that uses the dual-frequency microwave polarimetric signals.From the ice cloud scenes identified by the 183.3 ± 3 GHz channel brightness temperature (Tb), we find that the scattering by frozen particles is highly polarized, with V-H polarimetric differences (PDs) being positive throughout the tropics and the winter hemisphere mid-latitude jet regions, including PDs from the GMI 89 and 166 GHz TBs, as well as the PD at 640 GHz from the ER-2 Compact Scanning Submillimeter-wave Imaging Radiometer (CoSSIR) during the TC4 campaign. Large polarization dominantly occurs mostly near convective outflow regions (i.e., anvils or stratiform precipitation), while the polarization signal is small inside deep convective cores as well as at the remote cirrus region. Neglecting the polarimetric signal would easily result in as large as 30 % error in ice water path retrievals. There is a universal bell curve in the PD-TBV relationship, where the PD amplitude peaks at ˜ 10 K for all three channels in the tropics and increases slightly with latitude (2-4 K). Moreover, the 166 GHz PD tends to increase in the case where a melting layer is beneath the frozen particles aloft in the atmosphere, while 89 GHz PD is less sensitive than 166 GHz to the melting layer. This property creates a unique PD feature for the identification of the melting layer and stratiform rain with passive sensors.Horizontally oriented non-spherical frozen particles are thought to produce the observed PD because of different ice scattering properties in the V and H polarizations. On the other hand, turbulent mixing within deep convective cores inevitably promotes the random orientation of these particles, a mechanism that works effectively in reducing the PD. The current GMI polarimetric measurements themselves cannot fully disentangle the possible mechanisms.

Microphysical Properties of Frozen Particles Inferred from Global Precipitation Measurement (GPM) Microwave Imager (GMI) Polarimetric Measurements

NASA Technical Reports Server (NTRS)

Gong, Jie; Wu, Dongliang

2017-01-01

Scattering differences induced by frozen particle microphysical properties are investigated, using the vertically (V) and horizontally (H) polarized radiances from the Global Precipitation Measurement (GPM) Microwave Imager (GMI) 89 and 166GHz channels. It is the first study on global frozen particle microphysical properties that uses the dual-frequency microwave polarimetric signals. From the ice cloud scenes identified by the 183.3 3GHz channel brightness temperature (TB), we find that the scatterings of frozen particles are highly polarized with V-H polarimetric differences (PD) being positive throughout the tropics and the winter hemisphere mid-latitude jet regions, including PDs from the GMI 89 and 166GHz TBs, as well as the PD at 640GHz from the ER-2 Compact Scanning Submillimeter-wave Imaging Radiometer (CoSSIR) during the TC4 campaign. Large polarization dominantly occurs mostly near convective outflow region (i.e., anvils or stratiform precipitation), while the polarization signal is small inside deep convective cores as well as at the remote cirrus region. Neglecting the polarimetric signal would result in as large as 30 error in ice water path retrievals. There is a universal bell-curve in the PD TB relationship, where the PD amplitude peaks at 10K for all three channels in the tropics and increases slightly with latitude. Moreover, the 166GHz PD tends to increase in the case where a melting layer is beneath the frozen particles aloft in the atmosphere, while 89GHz PD is less sensitive than 166GHz to the melting layer. This property creates a unique PD feature for the identification of the melting layer and stratiform rain with passive sensors. Horizontally oriented non-spherical frozen particles are thought to produce the observed PD because of different ice scattering properties in the V and H polarizations. On the other hand, changes in the ice microphysical habitats or orientation due to turbulence mixing can also lead to a reduced PD in the deep convective cores. The current GMI polarimetric measurements themselves cannot fully disentangle the possible mechanisms.

Relative influence upon microwave emissivity of fine-scale stratigraphy, internal scattering, and dielectric properties

USGS Publications Warehouse

England, A.W.

1976-01-01

The microwave emissivity of relatively low-loss media such as snow, ice, frozen ground, and lunar soil is strongly influenced by fine-scale layering and by internal scattering. Radiometric data, however, are commonly interpreted using a model of emission from a homogeneous, dielectric halfspace whose emissivity derives exclusively from dielectric properties. Conclusions based upon these simple interpretations can be erroneous. Examples are presented showing that the emission from fresh or hardpacked snow over either frozen or moist soil is governed dominantly by the size distribution of ice grains in the snowpack. Similarly, the thickness of seasonally frozen soil and the concentration of rock clasts in lunar soil noticeably affect, respectively, the emissivities of northern latitude soils in winter and of the lunar regolith. Petrophysical data accumulated in support of the geophysical interpretation of microwave data must include measurements of not only dielectric properties, but also of geometric factors such as finescale layering and size distributions of grains, inclusions, and voids. ?? 1976 Birkha??user Verlag.

Protein and solute distribution in drug substance containers during frozen storage and post-thawing: a tool to understand and define freezing-thawing parameters in biotechnology process development.

PubMed

Kolhe, Parag; Badkar, Advait

2011-01-01

Active pharmaceutical ingredient for biotechnology-based drugs, commonly known as drug substance (DS), is often stored frozen for longer shelf-life. Freezing DS enhances stability by slowing down reaction rates that lead to protein instability, minimizes the risk of microbial growth, and eliminates the risk of transport-related stress. High density polyethylene bottles are commonly used for storing monoclonal antibody DS due to good mechanical stress/strain resistant properties even at low temperatures. Despite the aforementioned advantages for frozen storage of DS, this is not devoid of risks. Proteins are known to undergo ice-water surface denaturation, cryoconcentration, and cold denaturation during freezing. A systematic investigation was performed to better understand the protein and solute distribution along with potential of aggregate formation during freeze and thaw process. A significant solute and protein concentration gradient was observed for both frozen and thawed DS bottles. In case of thawed DS, cryoconcentration was localized in the bottom layer and a linear increase in concentration as a function of liquid depth was observed. On the other hand, for frozen DS, a "bell shaped" cryoconcentration distribution was observed between the bottom layers and centre position. A cryoconcentration of almost three-fold was observed for frozen DS in the most concentrated part when freezing was conducted at -20 and -40 °C and 2.5-fold cryoconcentration was observed in the thawed DS before mixing. The information obtained in this study is critical to design freeze thaw experiments, storage condition determination, and process improvement in manufacturing environment. Copyright © 2011 American Institute of Chemical Engineers (AIChE).

Total reflection infrared spectroscopy of water-ice and frozen aqueous NaCl solutions.

PubMed

Walker, Rachel L; Searles, Keith; Willard, Jesse A; Michelsen, Rebecca R H

2013-12-28

Liquid-like and liquid water at and near the surface of water-ice and frozen aqueous sodium chloride films were observed using attenuated total reflection infrared spectroscopy (ATR-IR). The concentration of NaCl ranged from 0.0001 to 0.01 M and the temperature varied from the melting point of water down to 256 K. The amount of liquid brine at the interface of the frozen films with the germanium ATR crystal increased with salt concentration and temperature. Experimental spectra are compared to reflection spectra calculated for a simplified morphology of a uniform liquid layer between the germanium crystal and the frozen film. This morphology allows for the amount of liquid observed in an experimental spectrum to be converted to the thickness of a homogenous layer with an equivalent amount of liquid. These equivalent thickness ranges from a nanometer for water-ice at 260 K to 170 nm for 0.01 M NaCl close to the melting point. The amounts of brine observed are over an order of magnitude less than the total liquid predicted by equilibrium thermodynamic models, implying that the vast majority of the liquid fraction of frozen solutions may be found in internal inclusions, grain boundaries, and the like. Thus, the amount of liquid and the solutes dissolved in them that are available to react with atmospheric gases on the surfaces of snow and ice are not well described by thermodynamic equilibrium models which assume the liquid phase is located entirely at the surface.

Effects of Corrugated Temperature Sheets on Optical Propagation along Quasi-Horizontal Paths in the Stably Stratified Atmosphere

DTIC Science & Technology

2015-12-11

diameter) are consistent with theoretical predictions based on Taylor’s frozen- turbulence hypothesis and the geometrical - optics approximation. Short...theoretical predictions based on Taylor’s frozen- turbulence hypothesis and the geometrical - optics approximation. Short-term (less than a few seconds... turbulent , quasi-horizontal interfaces, or “sheets”. Collocated in- situ and optical field measurements conducted in the atmospheric surface layer

Neoproterozoic sand wedges: crack formation in frozen soils under diurnal forcing during a snowball Earth

NASA Astrophysics Data System (ADS)

Maloof, Adam C.; Kellogg, James B.; Anders, Alison M.

2002-11-01

Thermal contraction cracking of permafrost produced sand-wedge polygons at sea level on the paleo-equator during late Neoproterozoic glacial episodes. These sand wedges have been used as evidence for high (≥54°) paleo-obliquity of the Earth's ecliptic, because cracks that form wedges are hypothesized to require deep seasonal cooling so the depth of the stressed layer in the ground reaches ≥1 m, similar to the measured depths of cracks that form wedges. To test the counter hypothesis that equatorial cracks opened under a climate characterized by a strong diurnal cycle and low mean annual temperature (snowball Earth conditions), we examine crack formation in frozen ground subject to periodic temperature variations. We derive analytical expressions relating the Newtonian viscosity to the potential crack depth, concluding that cracks will form only in frozen soils with viscosities greater than ˜10 14 Pa s. We also show numerical calculations of crack growth in frozen soils with stress- and temperature-dependent rheologies and find that fractures may propagate to depths 3-25 times the depth of the thermally stressed layer in equatorial permafrost during a snowball Earth because the mean annual temperature is low enough to keep the ground cold and brittle to relatively great depths.

40 CFR 407.43 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true [Reserved] 407.43 Section 407.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 405.81 - Specialized definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. 405.81 Section 405.81 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties...

40 CFR 407.43 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true [Reserved] 407.43 Section 407.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 407.43 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true [Reserved] 407.43 Section 407.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 407.43 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false [Reserved] 407.43 Section 407.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 407.43 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true [Reserved] 407.43 Section 407.43 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

[Influence of S-nitrosoglutathione on agglutination and nitric oxide concentration in frozen platelets].

PubMed

Wu, Tao; Liu, Jing-Han; Li, Hui; Zhou, Wu; Wang, Shu-Ying

2012-04-01

The aim of this study was to investigate the influence of S-nitrosoglutathione (GSNO) on agglutination and nitric oxide (NO) concentration in frozen platelets. The agglutination of platelets was detected by using platelet agglutination apparatus, the level of NO in platelets was detected by the nitrate enzyme reduction method. The results showed that the rates of agglutination in freeze platelets and frozen platelets treated with GSNO were (35.47 ± 2.93) and (24.43 ± 3.07), which were significantly lower than that in fresh liquid platelets (63.44 ± 2.96). The level of NO concentration in frozen platelets was (22.16 ± 6.38), which was significantly lower than that in fresh liquid platelets (31.59 ± 16.88). The level of NO concentration in frozen platelets treated with GSNO was (45.64 ± 6.31), which was significantly higher than that in fresh liquid platelets (P < 0.01). It is concluded that GSNO increases the concentration of NO in frozen platelets, inhibits platelet activation and maintains platelet function, thus GSNO can be used as a frozen protective agent.

40 CFR 407.41 - Specialized definitions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 407.41 Section 407.41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 405.83 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true [Reserved] 405.83 Section 405.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts...

40 CFR 405.83 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true [Reserved] 405.83 Section 405.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts...

40 CFR 405.81 - Specialized definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. 405.81 Section 405.81 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other...

40 CFR 405.81 - Specialized definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. 405.81 Section 405.81 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other...

40 CFR 405.83 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true [Reserved] 405.83 Section 405.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts...

40 CFR 405.83 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true [Reserved] 405.83 Section 405.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts...

40 CFR 405.83 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false [Reserved] 405.83 Section 405.83 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts, Novelties and Other Dairy Desserts...

40 CFR 407.41 - Specialized definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. 407.41 Section 407.41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 407.41 - Specialized definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. 407.41 Section 407.41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

40 CFR 407.41 - Specialized definitions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Specialized definitions. 407.41 Section 407.41 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen Potato Products...

Sensitization of thermotolerant SCK cells to hyperthermia and freezing with reduction of intracellular pH: implications for cryosurgery.

PubMed

Burgher, Abram H; Swanlund, David J; Griffin, Robert J; Song, Chang W; Bischof, John C; Roberts, Kenneth P

2003-03-01

During cryosurgery, cells frozen slowly at the outer part of the ice ball undergo severe dehydration and are subject to solute effects injury, which may be caused in part by protein denaturation. This study was undertaken to determine whether heat shock proteins (HSPs), the molecular chaperones that stabilize proteins against denaturation, have a protective effect on cells during slow freezing. In addition, we aimed to determine whether acidic conditions, similar to those found in many solid tumors, would effect this protection. SCK cells were frozen at 5 degrees C/min to -10 degrees C or -20 degrees C before or after induction of thermotolerance, and at neutral or low pH conditions. Lethal damage was determined by clonogenics. Clonogenic survival was decreased by 50% in thermotolerant cells frozen to -10 degrees C after culture in acidic conditions (pH 6.6) compared with non-thermotolerant cells cultured at neutral pH. Induction of thermotolerance alone or low pH alone did not significantly sensitize SCK cells to freezing. All treatment groups were equally susceptible to killing when frozen to -20 degrees C. Our results show that induction of thermal tolerance does not protect SCK cells against subsequent freezing injury and that a low pH environment actually sensitizes these cells to freeze injury. Copyright 2003 Wiley-Liss, Inc.

Comparison of the effect of raw and blanched-frozen broccoli on DNA damage in colonocytes.

PubMed

Lynn, Anthony; Fuller, Zoë; Collins, Andrew R; Ratcliffe, Brian

2015-07-01

Consumption of cruciferous vegetables may protect against colorectal cancer. Cruciferous vegetables are rich in a number of bioactive constituents including polyphenols, vitamins and glucosinolates. Before consumption, cruciferous vegetables often undergo some form of processing that reduces their content of bioactive constituents and may determine whether they exert protective effects. The aim of this study was to compare the ability of raw and blanched-frozen broccoli to protect colonocytes against DNA damage, improve antioxidant status and induce xenobiotic metabolizing enzymes (XME). Fifteen Landrace × Large White male pigs were divided into five age-matched and weight-matched sets (79 days, SD 3, and 34·7 kg, SD 3·9, respectively). Each set consisted of siblings to minimize genetic variation. Within each set, pigs received a cereal-based diet, unsupplemented (control) or supplemented with 600 g day(-1) of raw or blanched-frozen broccoli for 12 days. The consumption of raw broccoli caused a significant 27% increase in DNA damage in colonocytes (p = 0·03) relative to the control diet, whereas blanched-frozen broccoli had no significant effect. Both broccoli diets had no significant effect on plasma antioxidant status or hepatic and colonic XME. This study is the first to report that the consumption of raw broccoli can damage DNA in porcine colonocytes. Copyright © 2015 John Wiley & Sons, Ltd.

Frozen soil parameterization in a distributed biosphere hydrological model

NASA Astrophysics Data System (ADS)

Wang, L.; Koike, T.; Yang, K.; Jin, R.; Li, H.

2010-03-01

In this study, a frozen soil parameterization has been modified and incorporated into a distributed biosphere hydrological model (WEB-DHM). The WEB-DHM with the frozen scheme was then rigorously evaluated in a small cold area, the Binngou watershed, against the in-situ observations from the WATER (Watershed Allied Telemetry Experimental Research). First, by using the original WEB-DHM without the frozen scheme, the land surface parameters and two van Genuchten parameters were optimized using the observed surface radiation fluxes and the soil moistures at upper layers (5, 10 and 20 cm depths) at the DY station in July. Second, by using the WEB-DHM with the frozen scheme, two frozen soil parameters were calibrated using the observed soil temperature at 5 cm depth at the DY station from 21 November 2007 to 20 April 2008; while the other soil hydraulic parameters were optimized by the calibration of the discharges at the basin outlet in July and August that covers the annual largest flood peak in 2008. With these calibrated parameters, the WEB-DHM with the frozen scheme was then used for a yearlong validation from 21 November 2007 to 20 November 2008. Results showed that the WEB-DHM with the frozen scheme has given much better performance than the WEB-DHM without the frozen scheme, in the simulations of soil moisture profile at the cold regions catchment and the discharges at the basin outlet in the yearlong simulation.

Hydrothermal variations in soils resulting from the freezing and thawing processes in the active layer of an alpine grassland in the Qilian Mountains, northeastern Tibetan Plateau

NASA Astrophysics Data System (ADS)

Wang, Qingfeng; Yang, Qianqian; Guo, Hong; Xiao, Xiongxin; Jin, Huijun; Li, Lili; Zhang, Tingjun; Wu, Qingbai

2018-06-01

Soil hydrothermal dynamics, resulting from the freezing and thawing processes in the active layer and their influencing factors, were studied in the upper Heihe River Basin (UHRB) in the Qilian Mountains, northeastern Tibetan Plateau. Soil temperature and water content measurements were taken in the active layer of the UHRB in alpine grassland from 2013 to 2014. The results showed that the thaw rate of the active layer was significantly smaller in alpine paludal meadows than the thaw rate in alpine meadows and alpine steppes. This was mainly related to the hydrothermal properties of soils in the active layer, such as moisture content, thermal conductivity, and specific heat. During the thawing process, the active layer soil water content was higher and fluctuated less in alpine paludal meadows. Conversely, the soil water content was lower and fluctuated more significantly in alpine meadows and alpine steppes. These findings could be explained by the prevalence of peat soils, with a low bulk density, and high clay and organic matter content. By contrast, the soil particles in the active layer of alpine meadows and alpine steppes were significantly coarser, with higher bulk density and lower organic matter content. During the freezing process, gravel content and soil texture had a great impact on the unfrozen water content in the frozen soils. However, the factors influencing the soil water retention in frozen soils are complex, and further study is needed. These results provide theoretical support for the evaluation of the hydrological characteristics of the alpine permafrost zone in the Qilian Mountains. Furthermore, the effect of frozen ground on hydrological changes due to climate change in the Heihe River Basin can be simulated and predicted, providing a scientific basis for the ecological conservation of the Qilian Mountains National Park.

Total reflection infrared spectroscopy of water-ice and frozen aqueous NaCl solutions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walker, Rachel L.; Searles, Keith; Willard, Jesse A.

2013-12-28

Liquid-like and liquid water at and near the surface of water-ice and frozen aqueous sodium chloride films were observed using attenuated total reflection infrared spectroscopy (ATR-IR). The concentration of NaCl ranged from 0.0001 to 0.01 M and the temperature varied from the melting point of water down to 256 K. The amount of liquid brine at the interface of the frozen films with the germanium ATR crystal increased with salt concentration and temperature. Experimental spectra are compared to reflection spectra calculated for a simplified morphology of a uniform liquid layer between the germanium crystal and the frozen film. This morphologymore » allows for the amount of liquid observed in an experimental spectrum to be converted to the thickness of a homogenous layer with an equivalent amount of liquid. These equivalent thickness ranges from a nanometer for water-ice at 260 K to 170 nm for 0.01 M NaCl close to the melting point. The amounts of brine observed are over an order of magnitude less than the total liquid predicted by equilibrium thermodynamic models, implying that the vast majority of the liquid fraction of frozen solutions may be found in internal inclusions, grain boundaries, and the like. Thus, the amount of liquid and the solutes dissolved in them that are available to react with atmospheric gases on the surfaces of snow and ice are not well described by thermodynamic equilibrium models which assume the liquid phase is located entirely at the surface.« less

Pre-selection by double layer density gradient centrifugation improves the fertilising capacity of frozen-thawed, capacitated stallion sperm.

PubMed

Morató, Roser; Soares, Juleide M De Souza; Orero, Guifré; Mogas, Teresa; Miró, Jordi

2013-06-01

The effect of combining double layer density gradient centrifugation (DL-DGC) with different capacitation treatments on the fertilising capacity of frozen-thawed stallion sperm was examined via a heterologous assay involving in vitro-matured, zona pellucida-free bovine oocytes. In a first experiment, aliquots of frozen-thawed stallion sperm were subjected to one of five capacitation treatments without DL-DGC - ionomycin at 1.0μM, 0.1μM, 0.05μM or 0.01μM, or caffeine at 200μg/mL. The fertilising capacity of the semen was then assessed at 18h by staining the above oocytes with 4,6-diamidino-2-phenylindole (DAPI) and examining for sperm penetration, the number of penetrated spermatozoa per oocyte, and male pronucleus formation. In a second experiment, aliquots of frozen-thawed stallion sperm were subjected to DL-DGC selection - or not - and then further subjected to the two best capacitation treatments (0.1μM and 0.05μM ionomycin). The fertilising capacity of the semen was then determined as above. The DL-DGC/capacitated sperm samples showed the highest mean penetration rates: 24.16% following capacitation with 0.1μM ionomycin, and 12.21% following capacitation with 0.05μM ionomycin. The capacitated but non-DL-DGC-selected sperm returned significantly lower values: 6.26% and 7.02% for the same ionomycin treatments respectively. These findings suggest that combining DL-DGC selection with ionomycin capacitation improves the fertilising capacity of frozen-thawed stallion sperm. Copyright © 2013 Elsevier B.V. All rights reserved.

40 CFR 405.73 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false [Reserved] 405.73 Section 405.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory...

40 CFR 405.73 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true [Reserved] 405.73 Section 405.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory...

40 CFR 405.73 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true [Reserved] 405.73 Section 405.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory...

40 CFR 405.71 - Specialized definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Specialized definitions. 405.71 Section 405.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts...

40 CFR 405.84 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Pretreatment standards for existing sources. 405.84 Section 405.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.73 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true [Reserved] 405.73 Section 405.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory...

40 CFR 405.84 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Pretreatment standards for existing sources. 405.84 Section 405.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.71 - Specialized definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Specialized definitions. 405.71 Section 405.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts...

40 CFR 405.84 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Pretreatment standards for existing sources. 405.84 Section 405.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.84 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Pretreatment standards for existing sources. 405.84 Section 405.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.84 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Pretreatment standards for existing sources. 405.84 Section 405.84 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.73 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true [Reserved] 405.73 Section 405.73 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen Desserts Subcategory...

40 CFR 405.71 - Specialized definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Specialized definitions. 405.71 Section 405.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Fluid Mix for Ice Cream and Other Frozen...

40 CFR 407.46 - Pretreatment standards for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Pretreatment standards for new sources. 407.46 Section 407.46 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED FRUITS AND VEGETABLES PROCESSING POINT SOURCE CATEGORY Frozen...

40 CFR 405.85 - Standards of performance for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Standards of performance for new sources. 405.85 Section 405.85 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.86 - Pretreatment standards for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Pretreatment standards for new sources. 405.86 Section 405.86 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts...

40 CFR 405.86 - Pretreatment standards for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Pretreatment standards for new sources. 405.86 Section 405.86 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts...

40 CFR 405.85 - Standards of performance for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Standards of performance for new sources. 405.85 Section 405.85 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.86 - Pretreatment standards for new sources.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Pretreatment standards for new sources. 405.86 Section 405.86 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts...

40 CFR 405.86 - Pretreatment standards for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Pretreatment standards for new sources. 405.86 Section 405.86 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts...

40 CFR 405.86 - Pretreatment standards for new sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Pretreatment standards for new sources. 405.86 Section 405.86 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen Desserts...

40 CFR 405.85 - Standards of performance for new sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Standards of performance for new sources. 405.85 Section 405.85 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.85 - Standards of performance for new sources.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Standards of performance for new sources. 405.85 Section 405.85 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

40 CFR 405.85 - Standards of performance for new sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Standards of performance for new sources. 405.85 Section 405.85 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS DAIRY PRODUCTS PROCESSING POINT SOURCE CATEGORY Ice Cream, Frozen...

Freeze and Thaw of CD4+CD25+Foxp3+ Regulatory T Cells Results in Loss of CD62L Expression and a Reduced Capacity to Protect against Graft-versus-Host Disease

PubMed Central

Pierini, Antonio; Baker, Jeanette; Armstrong, Randall; Pan, Yuqiong; Leveson-Gower, Dennis; Negrin, Robert; Meyer, Everett

2015-01-01

The adoptive transfer of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in murine models of allogeneic hematopoietic cell transplantation (HCT) has been shown to protect recipient mice from lethal acute graft-versus-host disease (GVHD) and this approach is being actively investigated in human clinical trials. Here, we examined the effects of cryopreservation on Tregs. We found that freeze and thaw of murine and human Tregs is associated with reduced expression of L-selectin (CD62L), which was previously established to be an important factor that contributes to the in vivo protective effects of Tregs. Frozen and thawed murine Tregs showed a reduced capacity to bind to the CD62L binding partner MADCAM1 in vitro as well as an impaired homing to secondary lymphoid organs in vivo. Upon adoptive transfer frozen and thawed Tregs failed to protect against lethal GVHD compared with fresh Tregs in a murine model of allogeneic HCT across major histocompatibility barriers. In summary, the direct administration of adoptively transferred frozen and thawed Tregs adversely affects their immunosuppressive potential which is an important factor to consider in the clinical implementation of Treg immunotherapies. PMID:26693907

Effects of vitamin E and organic selenium on oxidative stability of omega-3 enriched dark chicken meat during cooking.

PubMed

Perez, T I; Zuidhof, M J; Renema, R A; Curtis, J M; Ren, Y; Betti, M

2010-03-01

The influence of vitamin E and selenomethionine (SeMet) on lipid oxidation in frozen-raw and cooked omega-3 enriched dark chicken meat was evaluated. Feed was supplemented with 2 levels of vitamin E (250 and 50 IU/kg of feed) and selenium (0.1 mg of sodium selenite/kg of feed and 0.3 mg of SeMet/kg of feed). An extruded linseed product was used as the alpha-linolenic acid source. Fatty acid (FA) profile, oxysterols, and thiobarbituric reactive acid substances (TBARs) were analyzed in frozen-raw, boiled, pan-fried, and roasted meat. After 6 mo of storage, oxysterols in frozen-raw meat remained stable with either high or low levels of dietary antioxidants. During cooking, high levels of vitamin E reduced oxysterol formation, whereas high levels of SeMet were inconsistent and even increased oxysterols during roasting. TBARs in frozen-raw meat stored for 6 mo were inhibited by high levels of either antioxidant. Conversely, no protective effect during cooking was observed at this time of storage. After 12 mo at -30 degrees C no antioxidant protection was observed.

Single-layer centrifugation through PureSperm® 80 selects improved quality spermatozoa from frozen-thawed dog semen.

PubMed

Dorado, J; Alcaraz, L; Gálvez, M J; Acha, D; Ortiz, I; Urbano, M; Hidalgo, M

2013-08-01

The aim of this study was to investigate whether single-layer centrifugation (SLC) with PureSperm® 80 could select good quality spermatozoa, including those with specific motility patterns, from doses of frozen dog semen. Semen from 5 dogs was collected and cryopreserved following a standard protocol. After thawing, semen samples were divided into two aliquots: one of them was used as control and the other one processed by SLC. Assessment of sperm motility (assessed by computer-assisted semen analysis), morphology (Diff-Quick staining) and viability (triple fluorescent stain of propidium iodine/isothiocyanate-labeled peanut (Arachis hypogaea) agglutinin/Rhodamine 123), were performed on aliquots of fresh semen, frozen-thawed control and frozen-thawed SLC treated samples. A multivariate clustering procedure separated 26,051 motile spermatozoa into three subpopulations (sP): sP1 consisting of highly active but non-progressive spermatozoa (40.3%), sP2 consisting of spermatozoa with high velocity and progressive motility (30.0%), and sP3 consisting of poorly active and non-progressive spermatozoa (29.7%). SLC with PureSperm® 80 yielded sperm suspensions with improved motility, morphology, viability and acrosome integrity (P<0.001). The frozen-thawed SLC treated samples were enriched in sP2, reaching a proportion of 44.1% of the present spermatozoa. From these results, we concluded that SLC with PureSperm® 80 may be an alternative and successful method for improving the quality of frozen-thawed dog spermatozoa. Moreover, sP2 (high-speed and progressive spermatozoa) was more frequently observed after SLC. Finally, this study also demonstrated that the general motile sperm structure present in dogs remained constant despite the effect caused by either cryopreservation or separation by SLC through PureSperm® 80. Copyright © 2013 Elsevier B.V. All rights reserved.

A Rosetta Stone for in situ Observations of Magnetic Reconnection

NASA Astrophysics Data System (ADS)

Scudder, J. D.; Daughton, W. S.; Karimabadi, H.; Roytershteyn, V.

2015-12-01

Local conditions that constrain the physics of magnetic reconnection in space in 3D will be discussed, including those observable conditions presently used and new ones that enhance experimental closure. Three classes of tests will be discussed: i) proxies for unmeasurable theoretical properties II) observable properties satisfied by all layers that pass mass flux, including those of the reconnection layer, and (iii) observable kinetic tests that are increasingly peculiar to collisionless magnetic reconnection. A Rosetta Stone of state of the art observables will be proposed, including proxies for unmeasurable theoretical local rate of frozen flux violation and measures of the significance of frozen flux encountered. A suite of kinetic observables involving properties peculiar to electrons will also be demonstrated as promising litmus tests for certifying sites of collisionless magnetic reconnection.

Improved cryopreservability of stallion sperm using a sorbitol-based freezing extender.

PubMed

Pojprasath, T; Lohachit, C; Techakumphu, M; Stout, T; Tharasanit, T

2011-06-01

Cryopreservation of stallion semen is often associated with poor post-thaw sperm quality. Sugars are among the important components of a freezing extender and act as non-permeating cryoprotectants. This study aimed to compare the quality of stallion sperm frozen with glucose, fructose or sorbitol-containing freezing extenders. Semen was collected from six stallions of proven fertility and cryopreserved using a freezing extender containing different types of monosaccharide sugars (glucose, fructose or sorbitol). After thawing, the semen was examined for sperm motility, viability, acrosome integrity, plasma membrane functionality and sperm longevity. The fertility of semen frozen in the presence of sorbitol was also tested by artificial insemination. Sperm quality was significantly decreased following freezing and thawing (P < 0.05). Fructose was inferior for protecting sperm during cryopreservation when compared to sorbitol and glucose (P < 0.05). Although the viability, motility and acrosome integrity of sperm cryopreserved with a glucose-containing extender did not significantly differ from sperm frozen in the sorbitol-based extender when examined at 2 and 4 h post-thaw, all of these parameters plus plasma membrane functionality were improved for sperm frozen in the sorbitol extender than in the glucose extender when examined 10 min post-thaw. Two of four mares (50%) inseminated with semen frozen with a sorbitol-containing freezing extender became pregnant. It is concluded that different sugars have different abilities to protect against cryoinjury during freezing and thawing of stallion sperm. This study demonstrated that an extender containing sorbitol as primary sugar can be used to successfully cryopreserve equine sperm; moreover, the quality of frozen-thawed sperm appeared to be better than when glucose or fructose was the principle sugar in the freezing extender. Copyright © 2011 Elsevier Inc. All rights reserved.

Efficacy of freezing, frozen storage and edible antimicrobial coatings used in combination for control of Listeria monocytogenes on roasted turkey stored at chiller temperatures.

PubMed

Jiang, Zheng; Neetoo, Hudaa; Chen, Haiqiang

2011-10-01

The presence and growth of Listeria monocytogenes on ready-to-eat (RTE) turkey is an important food safety issue. The antilisterial efficacy of four polysaccharide-based edible coatings (starch, chitosan, alginate and pectin) incorporating sodium lactate (SL) and sodium diacetate (SD) as well as commercial preparations Opti.Form PD4, NovaGARD™ CB1, Protect-M and Guardian™ NR100 were compared against L. monocytogenes on roasted turkey. Pectin coating treatments incorporating SL/SD, Opti.Form PD4 with or without Protect-M, and NovaGARD™ CB1 displayed higher antimicrobial efficacy against. L. monocytogenes than the other antimicrobials and coating materials. In the second phase of the study, it was investigated whether frozen storage could enhance the antilisterial effectiveness of pectin coating treatments on chilled roasted turkey. Inoculated roasted turkey samples coated with pectin-based treatments were frozen for up to 4 weeks and subsequently stored at 4 °C for 8 weeks. Frozen storage significantly enhanced the antilisterial activity of various coating treatments; with selected treatments reducing the L. monocytogenes populations by as much as 1.1 log CFU/cm(2) during the subsequent 8-week chilled storage. This study demonstrates that pectin-based antimicrobial edible coatings hold promise in enhancing the safety of RTE poultry products and frozen storage has the potential to enhance their effectiveness. Copyright © 2011 Elsevier Ltd. All rights reserved.

Quantifying the streamflow response to frozen ground degradation in the source region of the Yellow River within the Budyko framework

NASA Astrophysics Data System (ADS)

Wang, Taihua; Yang, Hanbo; Yang, Dawen; Qin, Yue; Wang, Yuhan

2018-03-01

The source region of the Yellow River (SRYR) is greatly important for water resources throughout the entire Yellow River Basin. Streamflow in the SRYR has experienced great changes over the past few decades, which is closely related to the frozen ground degradation; however, the extent of this influence is still unclear. In this study, the air freezing index (DDFa) is selected as an indicator for the degree of frozen ground degradation. A water-energy balance equation within the Budyko framework is employed to quantify the streamflow response to the direct impact of climate change, which manifests as changes in the precipitation and potential evapotranspiration, as well as the impact of frozen ground degradation, which can be regarded as part of the indirect impact of climate change. The results show that the direct impact of climate change and the impact of frozen ground degradation can explain 55% and 33%, respectively, of the streamflow decrease for the entire SRYR from Period 1 (1965-1989) to Period 2 (1990-2003). In the permafrost-dominated region upstream of the Jimai hydrological station, the impact of frozen ground degradation can explain 71% of the streamflow decrease. From Period 2 (1990-2003) to Period 3 (2004-2015), the observed streamflow did not increase as much as the precipitation; this could be attributed to the combined effects of increasing potential evapotranspiration and more importantly, frozen ground degradation. Frozen ground degradation could influence streamflow by increasing the groundwater storage when the active layer thickness increases in permafrost-dominated regions. These findings will help develop a better understanding of the impact of frozen ground degradation on water resources in the Tibetan Plateau.

P-wave and surface wave survey for permafrost analysis in alpine regions

NASA Astrophysics Data System (ADS)

Godio, A.; Socco, L. V.; Garofalo, F.; Arato, A.; Théodule, A.

2012-04-01

In various high mountain environments the estimate of mechanical properties of slope and sediments are relevant for the link of the geo-mechanical properties with the climate change effects. Two different locations were selected to perform seismic and georadar surveying, the Tsanteleina glacier (Gran Paradiso) and the Blue Lake in Val d'Ayas in the massif of Monterosa. The analysis of the seismic and GPR lines allowed to characterize the silty soil (top layer) and underlying bedrock. We applied seismic survey in time lapse mode to check the presence of "active" layer and estimate the mechanical properties of the moraines material and their sensitivity to the permafrost changes. Mechanical properties of sediments and moraines in glacial areas are related to the grain-size, the compaction of the material subjected to the past glacial activity, the presence of frozen materials and the reactivity of the permafrost to the climate changes. The test site of Tsanteleina has been equipped with sensors to monitor the temperature of soil and air and with time domain reflectometry to estimate the soil moisture and the frozen and thawing cycle of the uppermost material. Seismic reflections from the top of the permafrost layer are difficult to identify as they are embedded in the source-generated noise. Therefore we estimate seismic velocities from the analysis of traveltime refraction tomography and the analysis of surface wave. This approach provides information on compressional and shear waves using a single acquisition layout and a hammer acts as source. This reduces the acquisition time in complex logistical condition especially in winter period. The seismic survey was performed using 48 vertical geophones with 2 m spacing. The survey has been repeated in two different periods: summer 2011 and winter 2011. Common offset reflection lines with a 200 MHz GPR system (in summer) permitted to investigate the sediments and obtain information on the subsoil layering. The processing of seismic data involved the tomographic interpretation of traveltime P-wave first arrivals by considering the continuous refraction of the ray-paths. Several surface-wave dispersion curves were extracted in f-k domain along the seismic line and then inverted through a laterally constrained inversion algorithm to obtain a pseudo-2D section of S-wave velocity. Georadar investigation (about 2 km of georadar lines in the first site) confirmed the presence both of fine and coarse sediments in the uppermost layer; the seismic data allowed the moraines to be characterized down to 20-25 meters of depth. At the elevation of 2700 m asl, we observed a general decrease of the P-wave traveltimes collected in November, when the near surface layer was in frozen condition, respect to the data acquired in June. The frozen layer is responsible of the inversion of P-wave velocity with depth; the higher velocity layer (frozen) cannot be detected in the tomographic interpretation of refraction tomographic of the P-wave arrivals. Compressional wave velocity ranges from 700 m/s on the uppermost part, to 2000-2500 m/s in the internal part of the sediments reaching values higher than 5000 m/s at depth about 20 m. The analysis of surface wave permitted to estimate a slight increase from summer to winter of the S-wave velocity, in the depth range between 0 to 5 m.

Hydrological Changes in the Arctic in Response to a Changing Climate

NASA Astrophysics Data System (ADS)

Hinzman, L. D.; Kane, D. L.; McNamara, J. P.; Nolan, M. A.; Romanovsky, V. E.; Yang, D.; Yoshikawa, K.

2003-12-01

The broadest impacts of climate change to the terrestrial arctic regions will result through consequent effects of changing permafrost structure and extent. As the climate differentially warms in summer and winter, the permafrost will become warmer, the active layer (the layer of soil above the permafrost that annually experiences freeze and thaw) will become thicker, the lower boundary of permafrost will become shallower and permafrost extent will decrease in area. These simple structural changes will affect every aspect of the surface water and energy balances. As the active layer thickens, there is greater storage capacity for soil moisture and greater lags and decays are introduced into the hydrologic response times to precipitation. When the frozen ground is very close to the surface, the stream and river discharge peaks are higher and the base flow is lower. As permafrost becomes thinner, there can be more connections between surface and subsurface water. As permafrost extent decreases, there is more infiltration to groundwater. This has significant impacts on large and small scales. The timing of stream runoff will change, reducing the percentage of continental runoff released during the summer and increasing the proportion of winter runoff. This is already becoming evident in Siberian Rivers. As permafrost becomes thinner and is reduced in spatial extent, the proportions of groundwater in stream runoff will increase as the proportion of surface runoff decreases, increasing river alkalinity and electrical conductivity. This could impact mixing of fresh and saline waters, formation of the halocline and seawater chemistry. Other important impacts will occur due to changing basin geomorphology. Currently the drainage networks in arctic watersheds are quite immature as compared to the more well-developed stream networks of temperate regions. These stream channels are essentially frozen in place as the major flood events (predominantly snowmelt) occur when the soils and streambeds are frozen solid. As the active layer becomes thicker, there could be significantly increased sediment loads delivered to the ocean. Presently, most small streams (<=ssim1,000 km2) in the Arctic are completely frozen from the bed to the surface when spring melt is initiated. However, in lower reaches of the rivers there are places where the channel is deep enough to prevent complete winter freezing. Break-up of the rivers differs dramatically in these places where the ice is not frozen fast to the bottom. Huge ice chunks are lifted by the flowing water, chewing up channels bottoms and sides and introducing massive sediments to the spring runoff.

Multiphysics Modeling of Microwave Heating of a Frozen Heterogeneous Meal Rotating on a Turntable.

PubMed

Pitchai, Krishnamoorthy; Chen, Jiajia; Birla, Sohan; Jones, David; Gonzalez, Ric; Subbiah, Jeyamkondan

2015-12-01

A 3-dimensional (3-D) multiphysics model was developed to understand the microwave heating process of a real heterogeneous food, multilayered frozen lasagna. Near-perfect 3-D geometries of food package and microwave oven were used. A multiphase porous media model combining the electromagnetic heat source with heat and mass transfer, and incorporating phase change of melting and evaporation was included in finite element model. Discrete rotation of food on the turntable was incorporated. The model simulated for 6 min of microwave cooking of a 450 g frozen lasagna kept at the center of the rotating turntable in a 1200 W domestic oven. Temperature-dependent dielectric and thermal properties of lasagna ingredients were measured and provided as inputs to the model. Simulated temperature profiles were compared with experimental temperature profiles obtained using a thermal imaging camera and fiber-optic sensors. The total moisture loss in lasagna was predicted and compared with the experimental moisture loss during cooking. The simulated spatial temperature patterns predicted at the top layer was in good agreement with the corresponding patterns observed in thermal images. Predicted point temperature profiles at 6 different locations within the meal were compared with experimental temperature profiles and root mean square error (RMSE) values ranged from 6.6 to 20.0 °C. The predicted total moisture loss matched well with an RMSE value of 0.54 g. Different layers of food components showed considerably different heating performance. Food product developers can use this model for designing food products by understanding the effect of thickness and order of each layer, and material properties of each layer, and packaging shape on cooking performance. © 2015 Institute of Food Technologists®

Change in frozen soils and its effect on regional hydrology, upper Heihe basin, northeastern Qinghai-Tibetan Plateau

NASA Astrophysics Data System (ADS)

Gao, Bing; Yang, Dawen; Qin, Yue; Wang, Yuhan; Li, Hongyi; Zhang, Yanlin; Zhang, Tingjun

2018-02-01

Frozen ground has an important role in regional hydrological cycles and ecosystems, particularly on the Qinghai-Tibetan Plateau (QTP), which is characterized by high elevations and a dry climate. This study modified a distributed, physically based hydrological model and applied it to simulate long-term (1971-2013) changes in frozen ground its the effects on hydrology in the upper Heihe basin, northeastern QTP. The model was validated against data obtained from multiple ground-based observations. Based on model simulations, we analyzed spatio-temporal changes in frozen soils and their effects on hydrology. Our results show that the area with permafrost shrank by 8.8 % (approximately 500 km2), predominantly in areas with elevations between 3500 and 3900 m. The maximum depth of seasonally frozen ground decreased at a rate of approximately 0.032 m decade-1, and the active layer thickness over the permafrost increased by approximately 0.043 m decade-1. Runoff increased significantly during the cold season (November-March) due to an increase in liquid soil moisture caused by rising soil temperatures. Areas in which permafrost changed into seasonally frozen ground at high elevations showed especially large increases in runoff. Annual runoff increased due to increased precipitation, the base flow increased due to changes in frozen soils, and the actual evapotranspiration increased significantly due to increased precipitation and soil warming. The groundwater storage showed an increasing trend, indicating that a reduction in permafrost extent enhanced the groundwater recharge.

Snowmelt and Infiltration Deficiencies of SSiB and Their Resolution with a New Snow-Physics Scheme

NASA Technical Reports Server (NTRS)

Sud, Y. C.; Mocko, David M.

1999-01-01

A two-year 1987-1988 integration of SSiB forced with ISLSCP Initiative I surface data (as part of the Global Soil Wetness Project, GSWP, evaluation and intercomparison) produced generally realistic land surface fluxes and hydrology. Nevertheless, the evaluation also helped to identify some of the deficiencies of the current version of the Simplified Simple Biosphere (SSiB) model. The simulated snowmelt was delayed in most regions, along with excessive runoff and lack of an spring soil moisture recharge. The SSIB model had previously been noted to have a problem producing accurate soil moisture as compared to observations in the Russian snowmelt region. Similarly, various GSWP implementations of SSIB found deficiencies in this region of the simulated soil moisture and runoff as compared to other non-SSiB land-surface models (LSMs). The origin of these deficiencies was: 1) excessive cooling of the snow and ground, and 2) deep frozen soil disallowing snowmelt infiltration. The problem was most severe in regions that experience very cold winters. In SSiB, snow was treated as a unified layer with the first soil layer, causing soil and snow to cool together in the winter months, as opposed to snow cover acting as an insulator. In the spring season, a large amount of heat was required to thaw a hard frozen snow plus deep soil layers, delaying snowmelt and causing meltwater to become runoff over the frozen soil rather than infiltrate into it.

Thermal properties and unfrozen water content of frozen volcanic ash as a modelling input parameters in mountainous volcanic areas

NASA Astrophysics Data System (ADS)

Kuznetsova, E.

2016-12-01

Volcanic eruptions are one of the major causes of the burial of ice and snow in volcanic areas. This has been demonstrated on volcanoes, e.g. in Iceland, Russia, USA and Chile, where the combination of a permafrost-favorable climate and a thin layer of tephra is sufficient to reduce the sub-tephra layer snow ablation substantially, even to zero, causing ground ice formation and permafrost aggradation. Many numerical models that have been used to investigate and predict the evolution of cold regions as the result of climatic changes are lacking the accurate data of the thermal properties —thermal conductivity, heat capacity, thermal diffusivity—of soils or debris layers involved. The angular shape of the fragments that make up ash and scoria makes it inappropriate to apply existing models to estimate bulk thermal conductivity. The lack of experimental data on the thermal conductivity of volcanic deposits will hinder the development of realistic models. The decreasing thermal conductivity of volcanic ash in the frozen state is associated with the development and presence of unfrozen water films that may have a direct mechanical impact on the movement or slippage between ice and particle, and thus, change the stress transfer. This becomes particularly significant during periods of climate change when enhanced temperatures and associated melting could weaken polythermal glaciers and affect areas with warm and discontinuous permafrost, and induce ice or land movements, perhaps on a catastrophic scale. In the presentation, we will summarize existing data regarding: (i) the thermal properties and unfrozen water content in frozen volcanic ash and cinder, (ii) the effects of cold temperatures on weathering processes of volcanic glass, (iii) the relationship between the mineralogy of frozen volcanic deposits and their thermal properties —and then discusses their significance in relation to the numerical modelling of glaciers and permafrost's thermal behavior.

Soil Variable Permeability and Water Phase Change Dynamics in a Wastewater Spray Irrigation Agricultural System Located in a Seasonably Cold Climate

NASA Astrophysics Data System (ADS)

Darnault, C. J. G.; Daniel, T. J.; Billy, G.; Hopkins, I.; Guo, L.; Jin, Z.; Gall, H. E.; Lin, H.

2017-12-01

The permeability of the upper meter of soils in frozen conditions, commonly referred to as the active layer, can vary exponentially given the time of year. Variable moisture contents along with temperature, radiation, and slope angle of the soil surface can result in variable depths of frozen soils, which can cause the formation of low permeability ice lenses well into the spring thaw period. The wastewater irrigation site known as the "Living Filter" located in State College, PA has been in continuous operation since 1962. On average 5500 m3/day of wastewater is applied to the site annually, even in the winter months when average temperatures can dip as low as -7 °C during the month of January. The Living Filter is not permitted to discharge to surface water and is intended to recharge the Spring Creek basin that directly underlies the site, therefore runoff from the site is not permitted. We hypothesize that water infiltrates the upper meter of the subsurface during the winter in several different ways such as preferential pathways in the ice layer created by plant stems and weak patches of ice thawed by the warm wastewater. 2D conceptual models of the phase change between ice and water in the soil were created in order to predict soil permeability and its change in temperature. The 2D conceptual models can be correlated between observed soil moisture content and soil temperature data in order to validate the model given spray irrigation and weather patterns. By determining the permeability of the frozen soils, irrigation practices can be adjusted for the winter months so as to reduce the risk of any accidental wastewater runoff. The impact of this study will result in a better understanding of the multiphase dynamics of the active layer and their implication on soil hydrology at the Living Filter and other seasonally frozen sites.

Historical and future changes of frozen ground in the upper Yellow River Basin

NASA Astrophysics Data System (ADS)

Wang, Taihua; Yang, Dawen; Qin, Yue; Wang, Yuhan; Chen, Yun; Gao, Bing; Yang, Hanbo

2018-03-01

Frozen ground degradation resulting from climate warming on the Tibetan Plateau has aroused wide concern in recent years. In this study, the maximum thickness of seasonally frozen ground (MTSFG) is estimated by the Stefan equation, which is validated using long-term frozen depth observations. The permafrost distribution is estimated by the temperature at the top of permafrost (TTOP) model, which is validated using borehole observations. The two models are applied to the upper Yellow River Basin (UYRB) for analyzing the spatio-temporal changes in frozen ground. The simulated results show that the areal mean MTSFG in the UYRB decreased by 3.47 cm/10 a during 1965-2014, and that approximately 23% of the permafrost in the UYRB degraded to seasonally frozen ground during the past 50 years. Using the climate data simulated by 5 General Circulation Models (GCMs) under the Representative Concentration Pathway (RCP) 4.5, the areal mean MTSFG is projected to decrease by 1.69 to 3.07 cm/10 a during 2015-2050, and approximately 40% of the permafrost in 1991-2010 is projected to degrade into seasonally frozen ground in 2031-2050. This study provides a framework to estimate the long-term changes in frozen ground based on a combination of multi-source observations at the basin scale, and this framework can be applied to other areas of the Tibetan Plateau. The estimates of frozen ground changes could provide a scientific basis for water resource management and ecological protection under the projected future climate changes in headwater regions on the Tibetan Plateau.

Pitting from Sublimation of Underlying Dry-Ice Layer

NASA Image and Video Library

2011-04-21

NASA Mars Reconnaissance Orbiter took these images of an area near Mars south pole where coalescing or elongated pits are interpreted as signs of an underlying deposit of frozen carbon dioxide, or dry ice.

Evaluation of spin freezing versus conventional freezing as part of a continuous pharmaceutical freeze-drying concept for unit doses.

PubMed

De Meyer, L; Van Bockstal, P-J; Corver, J; Vervaet, C; Remon, J P; De Beer, T

2015-12-30

Spin-freezing as alternative freezing approach was evaluated as part of an innovative continuous pharmaceutical freeze-drying concept for unit doses. The aim of this paper was to compare the sublimation rate of spin-frozen vials versus traditionally frozen vials in a batch freeze-dryer, and its impact on total drying time. Five different formulations, each having a different dry cake resistance, were tested. After freezing, the traditionally frozen vials were placed on the shelves while the spin-frozen vials were placed in aluminum vial holders providing radial energy supply during drying. Different primary drying conditions and chamber pressures were evaluated. After 2h of primary drying, the amount of sublimed ice was determined in each vial. Each formulation was monitored in-line using NIR spectroscopy during drying to determine the sublimation endpoint and the influence of drying conditions upon total drying time. For all tested formulations and applied freeze-drying conditions, there was a significant higher sublimation rate in the spin-frozen vials. This can be explained by the larger product surface and the lower importance of product resistance because of the much thinner product layers in the spin frozen vials. The in-line NIR measurements allowed evaluating the influence of applied drying conditions on the drying trajectories. Copyright © 2015 Elsevier B.V. All rights reserved.

Nonspherical and Spherical Characterization of Ice in Hurricane Erin for Wideband Passive Microwave Comparisons

NASA Technical Reports Server (NTRS)

Skofronick-Jackson, Gail; Holthaus, Eric; Albers, Cerese; Kim, Min-Jeong

2007-01-01

In order to better understand the characteristics of frozen cloud particles in hurricane systems, computed brightness temperatures were compared with radiometric observations of Hurricane Erin (2001) from the NASA ER-2 aircraft. The focus was oil the frozen particle microphysics and the high frequencies (2 85 GHz) that are particularly sensitive to frozen particles. Frozen particles in hurricanes are an indicator of increasing hurricane intensity. In fact "hot towers" associated with increasing hurricane intensity are composed of frozen ice cloud particles. (They are called hot towers because their column of air is warmer than the surrounding air temperature, but above about 5-7 km to the tops of the towers at 15-19 km, the cloud particles are frozen.) This work showed that indeed, one can model information about cloud ice particle characteristics and indicated that nonspherical ice shapes, instead of spherical particles, provided the best match to the observations. Overall, this work shows that while non-spherical particles show promise, selecting and modeling a proper ice particle parameterization can be difficult and additional in situ measurements are needed to define and validate appropriate parameterizations. This work is important for developing Global Precipitation Measurement (GPM) mission satellite algorithms for the retrieval of ice characteristics both above the melting layer, as in Hurricane Erin, and for ice particles that reach the surface as falling snow.

Independent technical support for the frozen soil barrier installation and operation at the Fukushima Daiichi Nuclear Power Station (F1 Site)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Looney, Brian B.; Jackson, Dennis G.; Truex, Michael J.

TEPCO is implementing a number of water countermeasures to limit the releases and impacts of contaminated water to the surrounding environment. The diverse countermeasures work together in an integrated manner to provide different types, and several levels, of protection. In general, the strategy represents a comprehensive example of a “defense in depth” concept that is used for nuclear facilities around the world. One of the key countermeasures is a frozen soil barrier encircling the damaged reactor facilities. The frozen barrier is intended to limit the flow of water into the area and provide TEPCO the ability to reduce the amountmore » of contaminated water that requires treatment and storage. The National Laboratory team supports the selection of artificial ground freezing and the incorporation of the frozen soil barrier in the contaminated water countermeasures -- the technical characteristics of a frozen barrier are relatively well suited to the Fukushima-specific conditions and the need for inflow reduction. Further, our independent review generally supports the TEPCO/Kajima design, installation strategy and operation plan.« less

78 FR 33345 - Certain Frozen Warmwater Shrimp From Malaysia: Preliminary Countervailing Duty Determination

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

... ``battered shrimp'' (see below). ``Battered shrimp'' is a shrimp-based product: (1) That is produced from... the dusting layer. When dusted in accordance with the definition of dusting above, the battered shrimp...

78 FR 33350 - Certain Frozen Warmwater Shrimp From Thailand: Preliminary Countervailing Duty Determination

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

... and prawns; and (7) certain ``battered shrimp'' (see below). ``Battered shrimp'' is a shrimp-based... of dusting above, the battered shrimp product is also coated with a wet viscous layer containing egg...

78 FR 33344 - Certain Frozen Warmwater Shrimp From India: Preliminary Countervailing Duty Determination

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

...; and (7) certain ``battered shrimp'' (see below). ``Battered shrimp'' is a shrimp-based product: (1... of dusting above, the battered shrimp product is also coated with a wet viscous layer containing egg...

Agriculture and Food: Critical Infrastructure and Key Resources Sector-Specific Plan as Input to the National Infrastructure Protection Plan

DTIC Science & Technology

2007-05-01

sliced egg sand- wich (closed-faced), frozen fish dinner, rabbit stew, shrimp-flavored instant noodles , venison jerky, buffalo burgers, alligator...meat or 2% or more poultry (e.g., chicken noodle ) Other products Cheese, onion, mushroom pizza; spaghetti sauces (less than 3% red meat), spaghetti...sauce with mushrooms and 2% meat, pork and beans, sliced egg sandwich (closed-faced), frozen fish dinner, rabbit stew, shrimp-flavored instant

Noncontact Infrared-Mediated Heat Transfer During Continuous Freeze-Drying of Unit Doses.

PubMed

Van Bockstal, Pieter-Jan; De Meyer, Laurens; Corver, Jos; Vervaet, Chris; De Beer, Thomas

2017-01-01

Recently, an innovative continuous freeze-drying concept for unit doses was proposed, based on spinning the vials during freezing. An efficient heat transfer during drying is essential to continuously process these spin frozen vials. Therefore, the applicability of noncontact infrared (IR) radiation was examined. The impact of several process and formulation variables on the mass of sublimed ice after 15 min of primary drying (i.e., sublimation rate) and the total drying time was examined. Two experimental designs were performed in which electrical power to the IR heaters, distance between the IR heaters and the spin frozen vial, chamber pressure, product layer thickness, and 5 model formulations were included as factors. A near-infrared spectroscopy method was developed to determine the end point of primary and secondary drying. The sublimation rate was mainly influenced by the electrical power to the IR heaters and the distance between the IR heaters and the vial. The layer thickness had the largest effect on total drying time. The chamber pressure and the 5 model formulations had no significant impact on sublimation rate and total drying time, respectively. This study shows that IR radiation is suitable to provide the energy during the continuous processing of spin frozen vials. Copyright © 2016 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.

Simulation of Soil Frost and Thaw Fronts Dynamics with Community Land Model 4.5

NASA Astrophysics Data System (ADS)

Gao, J.; Xie, Z.

2016-12-01

Freeze-thaw processes in soils, including changes in frost and thaw fronts (FTFs) , are important physical processes. The movement of FTFs affects soil water and thermal characteristics, as well as energy and water exchanges between land surface and the atmosphere, and then the land surface hydrothermal process. In this study, a two-directional freeze and thaw algorithm for simulating FTFs is incorporated into the community land surface model CLM4.5, which is called CLM4.5-FTF. The simulated FTFs depth and soil temperature of CLM4.5-FTF compared well with the observed data both in D66 station (permafrost) and Hulugou station (seasonally frozen soil). Because the soil temperature profile within a soil layer can be estimated according to the position of FTFs, CLM4.5 performed better in soil temperature simulation. Permafrost and seasonally frozen ground conditions in China from 1980 to 2010 were simulated using the CLM4.5-FTF. Numerical experiments show that the spatial distribution of simulated maximum frost depth by CLM4.5-FTF has seasonal variation obviously. Significant positive active-layer depth trends for permafrost regions and negative maximum freezing depth trends for seasonal frozen soil regions are simulated in response to positive air temperature trends except west of Black Sea.

Investigation on the Formation of Needle Frost. II,

DTIC Science & Technology

For example, clay forms mutual layers with the clay which had not yet frozen with ice. In the case of agar-agar, we obtained the same phenomenon from gelidium jelly as we do when making agar-agar. (Author)

78 FR 33342 - Certain Frozen Warmwater Shrimp From the Socialist Republic of Vietnam: Preliminary...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

... and prawns; and (7) certain ``battered shrimp'' (see below). ``Battered shrimp'' is a shrimp-based... of dusting above, the battered shrimp product is also coated with a wet viscous layer containing egg...

Ring Resonator for Detection of Melting Brine Under Shallow Subsurface of Mars

NASA Technical Reports Server (NTRS)

Ponchak, George E.; Jordan, Jennifer L.; Scardelletti, Maximillian C.

2016-01-01

Laboratory experimental evidence using Raman spectroscopy has shown that liquid brine may form below the shallow subsurface of Mars. A simpler experimental method to verify the presence of liquid brine or liquid water below Mars surface is needed. In this paper, a ring resonator is used to detect the phase change between frozen water and liquid water below a sandy soil that simulates the Mars surface. Experimental data shows that the ring resonator can detect the melting of thin layers of frozen brine or water up to 15 mm below the surface.

Transient analysis of spectrally asymmetric magnetic photonic crystals with ferromagnetic losses

NASA Astrophysics Data System (ADS)

Jung, K.-Y.; Donderici, B.; Teixeira, F. L.

2006-10-01

We analyze transient electromagnetic pulse propagation in spectrally asymmetric magnetic photonic crystals (MPCs) with ferromagnetic losses. MPCs are dispersion-engineered materials consisting of a periodic arrangement of misaligned anisotropic dielectric and ferromagnetic layers that exhibit a stationary inflection point in the (asymmetric) dispersion diagram and unidirectional frozen modes. The analysis is performed via a late-time stable finite-difference time-domain method (FDTD) implemented with perfectly matched layer (PML) absorbing boundary conditions, and extended to handle (simultaneously) dispersive and anisotropic media. The proposed PML-FDTD algorithm is based on a D - H and B - E combined field approach that naturally decouples the FDTD update into two steps, one involving the (anisotropic and dispersive) constitutive material tensors and the other involving Maxwell’s equations in a complex coordinate space (to incorporate the PML). For ferromagnetic layers, a fully dispersive modeling of the permeability tensor is implemented to include magnetic losses in a consistent fashion. The numerical results illustrate some striking properties of MPCs, such as wave slowdown (frozen modes), amplitude increase (pulse compression), and unidirectional characteristics. The numerical model is also used to investigate the sensitivity of the MPC response against excitation (frequency and bandwidth), material (ferromagnetic losses), and geometric (layer misalignment and thickness) parameter variations.

The effects of sulfur mustard exposure and freezing on transdermal penetration of tritiated water through ex vivo pig skin.

PubMed

Payne, O J; Graham, S J; Dalton, C H; Spencer, P M; Mansson, R; Jenner, J; Azeke, J; Braue, E

2013-02-01

The percutaneous absorption of tritiated water ((3)H(2)O) through sulfur mustard (SM) exposed abdominal pig skin was measured using in vitro Franz-type static diffusion cells. The barrier function to water permeation following exposure to liquid SM for 8 min and excision 3h later did not change significantly. A small, but statistically significant difference (P<0.05) in steady state penetration (Jss), permeability coefficient (Kp) and lag time (t(L)) of (3)H(2)O was observed between fresh skin and skin stored frozen (-20 °C) for up to two weeks. Steady-state penetration and Kp values were significantly higher (P < 0.05) in skin stored frozen compared with fresh skin. Fresh naïve skin had an average Kp of 1.65 × 10(-3) cm h(-1), whereas frozen naïve skin was 2.04 × 10(-3) cm h(-1). Fresh SM exposed skin had a mean Kp of 1.72 × 10(-3) cm h(-1), whereas frozen SM exposed skin was 2.31 × 10(-3) cm h(-1). Lag times were also shorter (P<0.05) in skin that had been stored frozen. Frozen, SM-exposed porcine abdominal skin may be used for in vitro penetration studies, but effects of treatment and storage on the barrier layer should be taken into account. Copyright © 2012 Elsevier Ltd. All rights reserved.

The case study of drillbit and borehole frozen water of the subglacial Lake Vostok, East Antarctica for microbial content

NASA Astrophysics Data System (ADS)

Bulat, Sergey; Doronin, Maxim; Dominique, Marie; Lipenkov, Vladimir; Lukin, Valery; Karlov, Denis; Demchenko, Leonid; Khilchenko, Margarita

The objective was to estimate microbial content and diversity in the subglacial Lake Vostok (buried beneath 4-km thick East Antarctic ice sheet) by studying the uppermost water layer which entered the borehole upon lake entry (February 5, 2012) and then shortly frozen within. The samples of so-called drillbit water frozen on a drill bit upon lake enter (RAE57) along with re-drilled so-called borehole-frozen water (RAE58) were provided for the study with the ultimate goal to discover the life in this extreme icy environment. The comprehensive analyses (constrained by Ancient DNA research criteria) of the first lake water samples - drillbit- (one sample) and borehole-frozen (3 different depths 5G-2N-3425, 3429 et 3450m), are nearly got finished. If the drillbit water sample was heavily polluted with drill fluid (at ratio 1:1), re-drilled borehole-frozen samples were proved to be rather clean but still strongly smelling kerosene and containing numerous micro-droplets of drill fluid making the ice non-transparent. The cell concentrations measured by flow cytofluorometry showed 167 cells per ml in the drillbit water sample while in borehole-frozen samples ranged from 5.5 (full-cylinder 3429m deep frozen water ice core) to 38 cells per ml (freeze-centre of 3450m deep moon-shape ice core). DNA analyses came up with total 44 bacterial phylotypes discovered by sequencing of different regions (v3-v5, v4-v8, v4-v6 et full-gene) of 16S rRNA genes. Amongst them all but two were considered to be contaminants (were present in our contaminant library, including drill fluid findings). The 1st remaining phylotype successfully passing all contamination criteria proved to be hitherto-unknown type of bacterium (group of clones, 3 allelic variants) showing less than 86% similarity with known taxa. Its phylogenetic assignment to bacterial divisions or lineages was also unsuccessful despite of the RDP has classified it belonging to OD1 uncultured Candidate Division. The 2nd phylotype was less remarkable and still dubious in terms of contamination. It was presented by just one clone and showed 93% similarity with Janthinobacterium sp of Oxalobacteraceae (Beta-Proteobacteria) - well-known ‘water-loving’ bacteria. No archaea were detected in lake water frozen samples. Thus, the unidentified and unclassified bacterial w123-10 phylotype for the first time discovered in the uppermost water layer in subglacial Lake Vostok might represent ingenious cell populations in the lake, making the life in the lake less elusive. The proof may come (as well as novel phylotype discoveries) with farther analyses (e.g., sample screening with w123-10-specific primers, 16S rRNA v4 region amplicon sequencing) of existing and newly requested moon-shape samples of borehole-frozen water which are on a way to laboratories. We are deeply grateful to Jean Robert Petit and Jean Martins, UJF-CNRS, Grenoble (France) for assistance in conducting some analyses.

75 FR 52718 - Certain Frozen Warmwater Shrimp From India: Final Results of Antidumping Duty Changed...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-27

...; and (8) certain battered shrimp. Dusted shrimp is a shrimp-based product: (1) That is produced from...; and (5) that is subjected to IQF freezing immediately after application of the dusting layer. Battered...

77 FR 73619 - Final Results of Antidumping Duty Changed Circumstances Review: Certain Frozen Warmwater Shrimp...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-11

... warmwater shrimp and prawns (HTSUS subheading 1605.20.10.40); (7) certain battered shrimp. Battered shrimp... of dusting above, the battered shrimp product is also coated with a wet viscous layer containing egg...

78 FR 33347 - Certain Frozen Warmwater Shrimp From Ecuador: Preliminary Negative Countervailing Duty Determination

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

... ``battered shrimp'' (see below). ``Battered shrimp'' is a shrimp-based product: (1) That is produced from..., the battered shrimp product is also coated with a wet viscous layer containing egg and/or milk, and...

75 FR 74684 - Notice of Final Results of Antidumping Duty Changed Circumstances Review: Certain Frozen...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-01

... battered shrimp. Dusted shrimp is a shrimp-based product: (1) That is produced from fresh (or thawed-from... subjected to IQF freezing immediately after application of the dusting layer. Battered shrimp is a shrimp...

78 FR 33346 - Certain Frozen Warmwater Shrimp From the People's Republic of China: Preliminary Countervailing...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

...; and (7) certain ``battered shrimp'' (see below). ``Battered shrimp'' is a shrimp-based product: (1... above, the battered shrimp product is also coated with a wet viscous layer containing egg and/or milk...

Saccharide antifreeze compositions

DOEpatents

Walters, Kent; Duman, John G; Serianni, Anthony S

2013-12-10

The invention provides an antifreeze glycolipid compounds and composition comprising a polysaccharide moiety of Formula I; ##STR00001## wherein D-Manp represents a D-mannopyranose moiety, D-Xylp represents a D-xylopyranose moiety, and n is about 5 to about 70; and one or more lipid moieties covalently linked to the polysaccharide moiety of Formula I or electrostatically associated with the polysaccaride moiety for Formula I. The antifreeze glycolipid compounds and compositions can be used for a variety of industrial, agricultural, medical, and cosmetic applications where recrystallization-inhibition, cyroprotection, or cryopreservation is desired. The antifreeze glycolipid compounds or compositions can be used as, for example, as cryoprotectants for tissue preservation and transplantation, improving the texture of processed frozen food and frozen meats, frostbit protection, crop protection, and green alternatives for land vehicle antifreeze and aircraft de-icing.

Object recognition using deep convolutional neural networks with complete transfer and partial frozen layers

NASA Astrophysics Data System (ADS)

Kruithof, Maarten C.; Bouma, Henri; Fischer, Noëlle M.; Schutte, Klamer

2016-10-01

Object recognition is important to understand the content of video and allow flexible querying in a large number of cameras, especially for security applications. Recent benchmarks show that deep convolutional neural networks are excellent approaches for object recognition. This paper describes an approach of domain transfer, where features learned from a large annotated dataset are transferred to a target domain where less annotated examples are available as is typical for the security and defense domain. Many of these networks trained on natural images appear to learn features similar to Gabor filters and color blobs in the first layer. These first-layer features appear to be generic for many datasets and tasks while the last layer is specific. In this paper, we study the effect of copying all layers and fine-tuning a variable number. We performed an experiment with a Caffe-based network on 1000 ImageNet classes that are randomly divided in two equal subgroups for the transfer from one to the other. We copy all layers and vary the number of layers that is fine-tuned and the size of the target dataset. We performed additional experiments with the Keras platform on CIFAR-10 dataset to validate general applicability. We show with both platforms and both datasets that the accuracy on the target dataset improves when more target data is used. When the target dataset is large, it is beneficial to freeze only a few layers. For a large target dataset, the network without transfer learning performs better than the transfer network, especially if many layers are frozen. When the target dataset is small, it is beneficial to transfer (and freeze) many layers. For a small target dataset, the transfer network boosts generalization and it performs much better than the network without transfer learning. Learning time can be reduced by freezing many layers in a network.

75 FR 47546 - Certain Frozen Warmwater Shrimp from the Socialist Republic of Vietnam: Preliminary Results of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-06

... subheading 1605.20.10.40); 7) certain dusted shrimp; and 8) certain battered shrimp. Dusted shrimp is a... after application of the dusting layer. Battered shrimp is a shrimp-based product that, when dusted in...

The temperature characteristics of biological active period of the peat soils of Bakchar swamp

NASA Astrophysics Data System (ADS)

Kiselev, M. V.; Dyukarev, E. A.; Voropay, N. N.

2018-01-01

The results of the study of the peculiarities of the temperature regime in the five basic ecosystems of oligotrophic bogs in the south taiga zone of Western Siberia in 2011-2016 are presented. The soil temperature regime was studied using the atmospheric-soil measuring complex at different depths from surface to 240 cm. All sites were divided into two groups according the bog water level: flooded sites (hollow and open fen) and drained sites (ridge, tall and low ryam). Waterlogged sites are better warmed in the summer period, and slowly freeze in the winter period. The analysis of the annual cycle of temperature showed that the maximum surface temperature is observed in July. The minimum temperature on the surface observed in February or January. The greatest temperature gradient was recorded in the upper 2 cm layer. The gradient at the open fen was -2 °C·cm-1 in February and 1.1 °C·cm-1 in October. The peak of formation of the seasonally frozen layer occurs at the end of autumn, beginning of winter. The degradation of the seasonally frozen layer was observed both from top and bottom, but degradation from the top is faster.

Seasonally frozen layer in natural and drained peatlands at the South of West Siberia, Russia

NASA Astrophysics Data System (ADS)

Dyukarev, Egor; Kiselev, Maxim; Voropay, Nadezhda; Preis, Yulia

2017-04-01

The temperature regime of soils in natural and drained peatlands at Bakchar bog located in the South Taiga zone of West Siberia is studied. Soil temperature for depths up to 320 cm was registered using autonomous temperature profile recorder during the period from August 2010 to September 2016. Maximal and minimal temperatures were registered at surface in July and February, consequently. Extreme soil temperatures at 320 cm depth shifts to December (maximum) and July (minimum) reducing absolute values. Annual peat soil temperature amplitude decrease with depth from 21,8 °C on surface to 1,1 °C at 320 cm. The analysis of daily, month and annual mean data of temperature in peat soil has shown that seasonally frozen layer was registered up to 20-60 cm depth. The duration of seasonally freeze layer existence varies from 130 to 180 days. Drained peatlands with the lowest water table have highest freeze depth. Soil at water-logged sedge-sphagnum fen in winter is warmer than soil in ryam ecosystem and mineral soil at upland. Maximal freezing depth in peatlands is up to 3 times lower than at drain areas.

Effect of olive leaf (Olea europea L.) extracts on protein and lipid oxidation of long-term frozen n-3 fatty acids-enriched pork patties.

PubMed

Botsoglou, Evropi; Govaris, Alexander; Ambrosiadis, Ioannis; Fletouris, Dimitrios; Botsoglou, Nikolas

2014-10-01

Our previous study has demonstrated the protective effects of olive leaf extracts on the oxidation of pork patties from n-3 fatty acid-enriched meat during refrigerated storage. The target of the present study was to examine these effects during frozen storage. Results showed that frozen storage accelerated (P=0.05) both lipid and protein oxidation in pork patties, but an addition of olive leaf extract at 200mg gallic acid equivalent/kg improved sensory attributes by delaying oxidation of lipids (reduction (P=0.05) of conjugated dienes, hydroperoxides and malondialdehyde), and of proteins (reduction (P=0.05) of protein carbonyls and inhibition (P=0.05) of the decrease of protein sulfhydryls). Copyright © 2014. Published by Elsevier Ltd.

Isothiocyanate concentrations and interconversion of sulforaphane to erucin in human subjects after consumption of commercial frozen broccoli compared to fresh broccoli.

PubMed

Saha, Shikha; Hollands, Wendy; Teucher, Birgit; Needs, Paul W; Narbad, Arjan; Ortori, Catharine A; Barrett, David A; Rossiter, John T; Mithen, Richard F; Kroon, Paul A

2012-12-01

Sulforaphane (a potent anticarcinogenic isothiocyanate derived from glucoraphanin) is widely considered responsible for the protective effects of broccoli consumption. Broccoli is typically purchased fresh or frozen and cooked before consumption. We compared the bioavailability and metabolism of sulforaphane from portions of lightly cooked fresh or frozen broccoli, and investigated the bioconversion of sulforaphane to erucin. Eighteen healthy volunteers consumed broccoli soups produced from fresh or frozen broccoli florets that had been lightly cooked and sulforaphane thio-conjugates quantified in plasma and urine. Sulforaphane bioavailability was about tenfold higher for the soups made from fresh compared to frozen broccoli, and the reduction was shown to be due to destruction of myrosinase activity by the commercial blanching-freezing process. Sulforaphane appeared in plasma and urine in its free form and as several thio-conjugates forms. Erucin N-acetyl-cysteine conjugate was a significant urinary metabolite, and it was shown that human gut microflora can produce sulforaphane, erucin, and their nitriles from glucoraphanin. The short period of blanching used to produce commercial frozen broccoli destroys myrosinase and substantially reduces sulforaphane bioavailability. Sulforaphane was converted to erucin and excreted in urine, and it was shown that human colonic flora were capable of this conversion. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Interface and thickness dependent domain switching and stability in Mg doped lithium niobate

DOE PAGES

Neumayer, Sabine M.; Ivanov, Ilia N.; Manzo, Michele; ...

2015-12-08

Controlling ferroelectric switching in Mg doped lithium niobate (Mg: LN) is of fundamental importance for optical device and domain wall electronics applications that require precise domain patterns. Stable ferroelectric switching has been previously observed in undoped LN layers above proton exchanged (PE) phases that exhibit reduced polarization, whereas PE layers have been found to inhibit lateral domain growth. Here, Mg doping, which is known to significantly alter ferroelectric switching properties including coercive field and switching currents, is shown to inhibit domain nucleation and stability in Mg: LN above buried PE phases that allow for precise ferroelectric patterning via domain growthmore » control. Furthermore, piezoresponse force microscopy (PFM) and switching spectroscopy PFM reveal that the voltage at which polarization switches from the "up" to the "down" state increases with increasing thickness in pure Mg: LN, whereas the voltage required for stable back switching to the original "up" state does not exhibit this thickness dependence. This behavior is consistent with the presence of an internal frozen defect field. The inhibition of domain nucleation above PE interfaces, observed in this study, is a phenomenon that occurs in Mg: LN but not in undoped samples and is mainly ascribed to a remaining frozen polarization in the PE phase that opposes polarization reversal. This reduced frozen depolarization field in the PE phase also influences the depolarization field of the Mg: LN layer above due to the presence of uncompensated polarization charge at the PE-Mg: LN boundary. Furthermore, these alterations in internal electric fields within the sample cause long-range lattice distortions in Mg: LN via electromechanical coupling, which were corroborated with complimentary Raman measurements.« less

Interface and thickness dependent domain switching and stability in Mg doped lithium niobate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Neumayer, Sabine M.; Rodriguez, Brian J., E-mail: gallo@kth.se, E-mail: brian.rodriguez@ucd.ie; Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4

2015-12-14

Controlling ferroelectric switching in Mg doped lithium niobate (Mg:LN) is of fundamental importance for optical device and domain wall electronics applications that require precise domain patterns. Stable ferroelectric switching has been previously observed in undoped LN layers above proton exchanged (PE) phases that exhibit reduced polarization, whereas PE layers have been found to inhibit lateral domain growth. Here, Mg doping, which is known to significantly alter ferroelectric switching properties including coercive field and switching currents, is shown to inhibit domain nucleation and stability in Mg:LN above buried PE phases that allow for precise ferroelectric patterning via domain growth control. Furthermore,more » piezoresponse force microscopy (PFM) and switching spectroscopy PFM reveal that the voltage at which polarization switches from the “up” to the “down” state increases with increasing thickness in pure Mg:LN, whereas the voltage required for stable back switching to the original “up” state does not exhibit this thickness dependence. This behavior is consistent with the presence of an internal frozen defect field. The inhibition of domain nucleation above PE interfaces, observed in this study, is a phenomenon that occurs in Mg:LN but not in undoped samples and is mainly ascribed to a remaining frozen polarization in the PE phase that opposes polarization reversal. This reduced frozen depolarization field in the PE phase also influences the depolarization field of the Mg:LN layer above due to the presence of uncompensated polarization charge at the PE-Mg:LN boundary. These alterations in internal electric fields within the sample cause long-range lattice distortions in Mg:LN via electromechanical coupling, which were corroborated with complimentary Raman measurements.« less

Melting Frozen Droplets Using Photo-Thermal Traps

NASA Astrophysics Data System (ADS)

Dash, Susmita; de Ruiter, Jolet; Varanasi, Kripa

2017-11-01

Ice buildup is an operational and safety hazard in wind turbines, power lines, and airplanes. While traditional de-icing methods are energy-intensive or environmentally unfriendly, passive anti-icing approach using superhydrophobic surfaces fails under humid conditions, which necessitates development of passive deicing methods. Here, we investigate a passive technique for deicing using a multi-layer surface design that can efficiently absorb and convert the incident solar radiation to heat. The corresponding increase in substrate temperature allows for easy removal of frozen droplets from the surface. We demonstrate the deicing performance of the designed surface both at very low temperatures, and under frost and snow coverage.

Calculations of electric currents in Europa

NASA Technical Reports Server (NTRS)

Colburn, D. S.; Reynolds, R. T.

1986-01-01

Electrical currents should flow in the Galilean satellite, Europa, because it is located in Jupiter's corotating magnetosphere. The possible magnitudes of these currents are calculated by assuming that Europa is a differentiated body consisting of an outer H2O layer and a silicate core. Two types of models are considered here: one in which the water is completely frozen and a second in which there is an intermediate liquid layer. For the transverse electric mode (eddy currents), the calculated current density in a liquid layer is approximately 10 to the -5/Am. For the transverse magnetic mode (unipolar generator), the calculated current density in the liquid is severely constrained by the ice layer to a range of only 10 to the -10 to -11th power/ Am, for a total H2O thickness of 100 km, provided that neither layer is less than 4 km thick. The current density is less for a completely frozen H2O layer. If transient cracks were to appear in the ice layer, thereby exposing liquid, the calculated current density could rise to a range of 10 to the -6 to 10 to the -5/Am, depending on layer thicknesses, which would require an exposed area of 10 to the -9 to 10 to the -8 of the Europa surface. The corresponding total current of 2.3x10 to the 5th power A could in 1 yr. electrolyze 7x10 to the 5th power kg of water (and more if the cells were in series), and thereby store up to 10 the 8th power J of energy, but it is not clear how electrolysis can take place in the absence of suitable electrodes. Electrical heating would be significant only if the ice-layer thickness were on the order of 1 m, such as might occur if an exposed liquid surface were to freeze over; the heating under this condition could hinder the thickening of the ice layer.

Chemically frozen multicomponent boundary layer theory of salt and/or ash deposition rates from combustion gases

NASA Technical Reports Server (NTRS)

Rosner, D. E.; Chen, B.-K.; Fryburg, G. C.; Kohl, F. J.

1979-01-01

There is increased interest in, and concern about, deposition and corrosion phenomena in combustion systems containing inorganic condensible vapors and particles (salts, ash). To meet the need for a computationally tractable deposition rate theory general enough to embrace multielement/component situations of current and future gas turbine and magnetogasdynamic interest, a multicomponent chemically 'frozen' boundary layer (CFBL) deposition theory is presented and its applicability to the special case of Na2SO4 deposition from seeded laboratory burner combustion products is demonstrated. The coupled effects of Fick (concentration) diffusion and Soret (thermal) diffusion are included, along with explicit corrections for effects of variable properties and free stream turbulence. The present formulation is sufficiently general to include the transport of particles provided they are small enough to be formally treated as heavy molecules. Quantitative criteria developed to delineate the domain of validity of CFBL-rate theory suggest considerable practical promise for the present framework, which is characterized by relatively modest demands for new input information and computer time.

Heat Transfer to Surfaces of Finite Catalytic Activity in Frozen Dissociated Hypersonic Flow

NASA Technical Reports Server (NTRS)

Chung, Paul M.; Anderson, Aemer D.

1961-01-01

The heat transfer due to catalytic recombination of a partially dissociated diatomic gas along the surfaces of two-dimensional and axisymmetric bodies with finite catalytic efficiencies is studied analytically. An integral method is employed resulting in simple yet relatively complete solutions for the particular configurations considered. A closed form solution is derived which enables one to calculate atom mass-fraction distribution, therefore catalytic heat transfer distribution, along the surface of a flat plate in frozen compressible flow with and without transpiration. Numerical calculations are made to determine the atom mass-fraction distribution along an axisymmetric conical body with spherical nose in frozen hypersonic compressible flow. A simple solution based on a local similarity concept is found to be in good agreement with these numerical calculations. The conditions are given for which the local similarity solution is expected to be satisfactory. The limitations on the practical application of the analysis to the flight of the blunt bodies in the atmosphere are discussed. The use of boundary-layer theory and the assumption of frozen flow restrict application of the analysis to altitudes between about 150,000 and 250,000 feet.

Heat transfer in nonequilibrium boundary layer flow over a partly catalytic wall

NASA Astrophysics Data System (ADS)

Wang, Zhi-Hui

2016-11-01

Surface catalysis has a huge influence on the aeroheating performance of hypersonic vehicles. For the reentry flow problem of a traditional blunt vehicle, it is reasonable to assume a frozen boundary layer surrounding the vehicles' nose, and the catalytic heating can be decoupled with the heat conduction. However, when considering a hypersonic cruise vehicle flying in the medium-density near space, the boundary layer flow around its sharp leading-edge is likely to be nonequilibrium rather than frozen due to rarefied gas effects. As a result, there will be a competition between the heat conduction and the catalytic heating. In this paper, the theoretical modeling and the direct simulation Monte Carlo (DSMC) method are employed to study the corresponding rarefied nonequilibrium flow and heat transfer phenomena near the leading edge of the near space hypersonic vehicles. It is found that even under identical rarefication degree, the nonequilibrium degree of the flow and the corresponding heat transfer performance of the sharp leading edges could be different from that of the big blunt noses. A generalized model is preliminarily proposed to describe and to evaluate the competitive effects between the homogeneous recombination of atoms inside the nonequilibrium boundary layer and the heterogeneous recombination of atoms on the catalytic wall surface. The introduced nonequilibrium criterion and the analytical formula are validated and calibrated by the DSMC results, and the physical mechanism is discussed.

Stagnation-point heat transfer correlation for ionized gases

NASA Technical Reports Server (NTRS)

Bade, W. L.

1975-01-01

Based on previous laminar boundary-layer solutions for argon, xenon, nitrogen, and air, it is shown that the effect of gas ionization on stagnation-point heat transfer can be correlated with the variation of the frozen Prandtl number across the boundary layer. A formula is obtained for stagnation-point heat transfer in a noble gas and is shown to be valid from the low-temperature range to the region of strong ionization. It is concluded that the considered effect can be well correlated by the 0.7 power of the Prandtl-number ratio across the boundary layer.

Antifreeze glycoproteins from antarctic notothenioid fishes fail to protect the rat cardiac explant during hypothermic and freezing preservation.

PubMed

Wang, T; Zhu, Q; Yang, X; Layne, J R; Devries, A L

1994-04-01

The antarctic notothenioid fishes avoid freezing through the action of circulating antifreeze glycoproteins (AFGPs). This study investigated whether AFGPs could serve as cryoprotectants for the isolated rat heart under three different storage conditions. (1) Hearts were flushed with 15 mg AFGP/ml cardioplegic solution (CP) and stored for 9 h at 0 degrees C. This AFGP concentration has been reported to protect pig oocytes during hypothermic storage. (2) Hearts were flushed with 10 mg AFGP/ml CP-14 and stored frozen at -1.4 degrees C for 3 h. At this concentration the AFGPs significantly reduce the solution freezing point and also change the crystal morphology from dendritic to spicular. (3) Hearts were flushed with 10 micrograms AFGP/ml CP-15 and stored frozen at -1.4 degrees C for 5 h. At this low concentration the AFGPs have a strong inhibitory effect on ice recrystallization, but have little effect on the freezing point and less apparent effect on the crystal habit. After hypothermic or freezing storage, the functional viability was assessed by determining cardiac output (CO) during working reperfusion. No difference in CO was found between AFGP-treated and untreated hearts after 9 h of 0 degree C storage. CO in hearts frozen in CP-14 without AFGPs recovered to 50% of the freshly perfused control hearts. Hearts frozen in the presence of high concentrations of AFGPs (10 mg/ml CP-14) failed to beat upon thawing and reperfusion, although their tissue ice content was less than that found in hearts without AFGP treatment.(ABSTRACT TRUNCATED AT 250 WORDS)

Breeding of Freeze-tolerant Yeast and the Mechanisms of Stress-tolerance

NASA Astrophysics Data System (ADS)

Hino, Akihiro

Frozen dough method have been adopted in the baking industry to reduce labor and to produce fresh breads in stores. New freeze-tolerant yeasts for frozen dough preparations were isolated from banana peel and identified. To obtain strains that have fermentative ability even after several months of frozen storage in fermented dough, we attempted to breed new freeze-tolerantstrain. The hybrid between S.cerevisiae, which is a isolated freeze-tolerant strain, and a strain isolated from bakers' yeast with sexual conjugation gave a good quality bread made from frozen dough method. Freeze-tolerant strains showed higher surviving and trehalose accumulating abilities than freeze-sensitive strains. The freeze tolerance of the yeasts was associated with the basal amount of intracellular trehalose after rapid degradation at the onset of the prefermentation period. The complicated metabolic pathway and the regulation system of trehalose in yeast cells are introduced. The trehalose synthesis may act as a metabolic buffer system which contribute to maintain the intracellular inorganic phosphate and as a feedback regulation system in the glycolysis. However, it is not known enough how the trehalose protects yeast cells from stress.

Physical-Chemical Basis of the Protection of Slowly Frozen Human Erythrocytes by Glycerol

PubMed Central

Rall, W. F.; Mazur, Peter; Souzu, Hiroshi

1978-01-01

One theory of freezing damage suggests that slowly cooled cells are killed by being exposed to increasing concentrations of electrolytes as the suspending medium freezes. A corollary to this view is that protective additives such as glycerol protect cells by acting colligatively to reduce the electrolyte concentration at any subzero temperature. Recently published phase-diagram data for the ternary system glycerol-NaCl-water by M. L. Shepard et al. (Cryobiology, 13:9-23, 1976), in combination with the data on human red cell survival vs. subzero temperature presented here and in the companion study of Souzu and Mazur (Biophys. J., 23:89-100), permit a precise test of this theory. Appropriate liquidus phase-diagram information for the solutions used in the red cell freezing experiments was obtained by interpolation of the liquidus data of Shepard and his co-workers. The results of phase-diagram analysis of red cell survival indicate that the correlation between the temperature that yields 50% hemolysis (LT50) and the electrolyte concentration attained at that temperature in various concentrations of glycerol is poor. With increasing concentrations of glycerol, the cells were killed at progressively lower concentrations of NaCl. For example, the LT50 for cells frozen in the absence of glycerol corresponds to a NaCl concentration of 12 weight percent (2.4 molal), while for cells frozen in 1.75 M glycerol in buffered saline the LT50 corresponds to 3.0 weight percent NaCl (1.3 molal). The data, in combination with other findings, lead to two conclusions: (a) The protection from glycerol is due to its colligative ability to reduce the concentration of sodium chloride in the external medium, but (b) the protection is less than that expected from colligative effects; apparently glycerol itself can also be a source of damage, probably because it renders the red cells susceptible to osmotic shock during thawing. PMID:667300

Further results on the stagnation point boundary layer with hydrogen injection.

NASA Technical Reports Server (NTRS)

Wu, P.; Libby, P. A.

1972-01-01

The results of an earlier paper on the behavior of the boundary layer at an axisymmetric stagnation with hydrogen injection into a hot external airstream are extended to span the entire range from essentially frozen to essentially equilibrium flow. This extension is made possible by the employment of finite difference methods; the accurate treatment of the boundary conditions at 'infinity,' the differencing technique employed and the formulation resulting in block tri-diagonal matrices are slight variants in the present work.

A method for the direct numerical simulation of hypersonic boundary-layer instability with finite-rate chemistry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marxen, Olaf, E-mail: olaf.marxen@vki.ac.be; Aeronautics and Aerospace Department, von Karman Institute for Fluid Dynamics, Chaussée de Waterloo, 72, 1640 Rhode-St-Genèse; Magin, Thierry E.

2013-12-15

A new numerical method is presented here that allows to consider chemically reacting gases during the direct numerical simulation of a hypersonic fluid flow. The method comprises the direct coupling of a solver for the fluid mechanical model and a library providing the physio-chemical model. The numerical method for the fluid mechanical model integrates the compressible Navier–Stokes equations using an explicit time advancement scheme and high-order finite differences. This Navier–Stokes code can be applied to the investigation of laminar-turbulent transition and boundary-layer instability. The numerical method for the physio-chemical model provides thermodynamic and transport properties for different gases as wellmore » as chemical production rates, while here we exclusively consider a five species air mixture. The new method is verified for a number of test cases at Mach 10, including the one-dimensional high-temperature flow downstream of a normal shock, a hypersonic chemical reacting boundary layer in local thermodynamic equilibrium and a hypersonic reacting boundary layer with finite-rate chemistry. We are able to confirm that the diffusion flux plays an important role for a high-temperature boundary layer in local thermodynamic equilibrium. Moreover, we demonstrate that the flow for a case previously considered as a benchmark for the investigation of non-equilibrium chemistry can be regarded as frozen. Finally, the new method is applied to investigate the effect of finite-rate chemistry on boundary layer instability by considering the downstream evolution of a small-amplitude wave and comparing results with those obtained for a frozen gas as well as a gas in local thermodynamic equilibrium.« less

Effects of a type I antifreeze protein (AFP) on the melting of frozen AFP and AFP+solute aqueous solutions studied by NMR microimaging experiment.

PubMed

Ba, Yong; Mao, Yougang; Galdino, Luiz; Günsen, Zorigoo

2013-01-01

The effects of a type I AFP on the bulk melting of frozen AFP solutions and frozen AFP+solute solutions were studied through an NMR microimaging experiment. The solutes studied include sodium chloride and glucose and the amino acids alanine, threonine, arginine, and aspartic acid. We found that the AFP is able to induce the bulk melting of the frozen AFP solutions at temperatures lower than 0 °C and can also keep the ice melted at higher temperatures in the AFP+solute solutions than those in the corresponding solute solutions. The latter shows that the ice phases were in super-heated states in the frozen AFP+solute solutions. We have tried to understand the first experimental phenomenon via the recent theoretical prediction that type I AFP can induce the local melting of ice upon adsorption to ice surfaces. The latter experimental phenomenon was explained with the hypothesis that the adsorption of AFP to ice surfaces introduces a less hydrophilic water-AFP-ice interfacial region, which repels the ionic/hydrophilic solutes. Thus, this interfacial region formed an intermediate chemical potential layer between the water phase and the ice phase, which prevented the transfer of water from the ice phase to the water phase. We have also attempted to understand the significance of the observed melting phenomena to the survival of organisms that express AFPs over cold winters.

Alaskan frozen soil impact tests of the B83-C/S and Strategic Earth Penetrator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dockery, H.A.; Clarke, J.B.; Stull, S.P.

To assess the penetrability of the B83 strategic bomb and a Strategic Earth Penetrator design into frozen soil and ice, Lawrence Livermore National Laboratory and Sandia National Laboratories, assisted by the US Air Force and US Army, conducted a series of tests in 1987. In April, Strategic Earth Penetrator units were dropped into multi-year sea ice and frozen tundra near Prudhoe Bay, Alaska. Calculated impact velocity ranged from 200 to 308 ft/s into ice and from 200 to 444 ft/s into frozen tundra. Tests in May include drops of a B83 design with specially designed ogive nose shape, a B83more » with a cap over the production ''cookie cutter'' nose, and a Strategic Earth Penetrator. The May tests were conducted near Fairbanks, Alaska, at Eielson Air Force Base and at Donnelly Flats on the Fort Greely Military Reservation. The type of frozen soil encountered at Eielson was very homogeneous in composition; however. Two drops impacted areas with very thin frozen soil layers at depths of about 24 in. below the surface. Velocities of these drops prior to impact ranged from 256 to 308 ft/s, and peak axial deceleration ranged from 160 to 490 g. The units penetrated to depths of 7.5-12 ft. Three other events impacted in a target area where frozen soil averaging 35 in. thick extended essentially to the surface. We calculated velocities prior to impact at 200-256 ft/s; and penetration depths of 3.2-9.6 ft. The geologic material at Donnelly Flats was primarily a very hard, rocky glacial deposit with a variable degree of ice bonding. Here, the test units dropped from 10,000 ft above ground level and achieved an average calculated velocity of 802 ft/s. Depth of penetration ranged from 7.6 to 13.5 ft.« less

Features of seasonal temperature variations in peat soils of oligotrophic bogs in south taiga of Western Siberia

NASA Astrophysics Data System (ADS)

Kiselev, M. V.; Dyukarev, E. A.; Voropay, N. N.

2018-03-01

The work presents the results of the study of the peculiarities of the temperature regime in the five basic ecosystems of oligotrophic bogs in the south taiga zone of Western Siberia in 2011-2016. The soil temperature regime was studied using the atmospheric-soil measuring complex at different depths from surface down to 240 cm. All sites were divided into two groups according to the bog water level: flooded sites (hollow and open fen) and drained sites (ridge, tall and low ryam). The waterlogged sites are better warmed in the summer period and slowly freeze in the winter period. The analysis of the annual cycle of temperature showed that the maximum surface temperature is in July. The minimum temperature on the surface is observed in February or January. The greatest temperature gradient was recorded in the upper 2 cm layer. The gradient at the open fen was -2 °C/cm in February and 1.1 °C/cm in October. The peak of formation of the seasonally frozen layer occurs at the end of autumn or in the beginning of winter. The degradation of the seasonally frozen layer was observed both from top and bottom, but the degradation rate from the top is faster.

Spatio-temporal characteristics of large scale motions in a turbulent boundary layer from direct wall shear stress measurement

NASA Astrophysics Data System (ADS)

Pabon, Rommel; Barnard, Casey; Ukeiley, Lawrence; Sheplak, Mark

2016-11-01

Particle image velocimetry (PIV) and fluctuating wall shear stress experiments were performed on a flat plate turbulent boundary layer (TBL) under zero pressure gradient conditions. The fluctuating wall shear stress was measured using a microelectromechanical 1mm × 1mm floating element capacitive shear stress sensor (CSSS) developed at the University of Florida. The experiments elucidated the imprint of the organized motions in a TBL on the wall shear stress through its direct measurement. Spatial autocorrelation of the streamwise velocity from the PIV snapshots revealed large scale motions that scale on the order of boundary layer thickness. However, the captured inclination angle was lower than that determined using the classic method by means of wall shear stress and hot-wire anemometry (HWA) temporal cross-correlations and a frozen field hypothesis using a convection velocity. The current study suggests the large size of these motions begins to degrade the applicability of the frozen field hypothesis for the time resolved HWA experiments. The simultaneous PIV and CSSS measurements are also used for spatial reconstruction of the velocity field during conditionally sampled intense wall shear stress events. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1315138.

Surface geophysical methods for characterising frozen ground in transitional permafrost landscapes

USGS Publications Warehouse

Briggs, Martin A.; Campbell, Seth; Nolan, Jay; Walvoord, Michelle Ann; Ntarlagiannis, Dimitrios; Day-Lewis, Frederick D.; Lane, John W.

2017-01-01

The distribution of shallow frozen ground is paramount to research in cold regions, and is subject to temporal and spatial changes influenced by climate, landscape disturbance and ecosystem succession. Remote sensing from airborne and satellite platforms is increasing our understanding of landscape-scale permafrost distribution, but typically lacks the resolution to characterise finer-scale processes and phenomena, which are better captured by integrated surface geophysical methods. Here, we demonstrate the use of electrical resistivity imaging (ERI), electromagnetic induction (EMI), ground penetrating radar (GPR) and infrared imaging over multiple summer field seasons around the highly dynamic Twelvemile Lake, Yukon Flats, central Alaska, USA. Twelvemile Lake has generally receded in the past 30 yr, allowing permafrost aggradation in the receded margins, resulting in a mosaic of transient frozen ground adjacent to thick, older permafrost outside the original lakebed. ERI and EMI best evaluated the thickness of shallow, thin permafrost aggradation, which was not clear from frost probing or GPR surveys. GPR most precisely estimated the depth of the active layer, which forward electrical resistivity modelling indicated to be a difficult target for electrical methods, but could be more tractable in time-lapse mode. Infrared imaging of freshly dug soil pit walls captured active-layer thermal gradients at unprecedented resolution, which may be useful in calibrating emerging numerical models. GPR and EMI were able to cover landscape scales (several kilometres) efficiently, and new analysis software showcased here yields calibrated EMI data that reveal the complicated distribution of shallow permafrost in a transitional landscape.

Cold Regions Environmental Considerations

DTIC Science & Technology

2009-02-03

braided streams, variable discharge, seasonal breakup) limited seasonally limited abundant Hydrology (frozen lakes and bogs) not present seasonally...fuel hoses may crack increasing the potential for fuel spills. Extreme care must be used when handling cables at cold temperatures, protecting the

Submerged combustion melting processes for producing glass and similar materials, and systems for carrying out such processes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Charbonneau, Mark William

Processes of controlling submerged combustion melters, and systems for carrying out the methods. One process includes feeding vitrifiable material into a melter vessel, the melter vessel including a fluid-cooled refractory panel in its floor, ceiling, and/or sidewall, and heating the vitrifiable material with a burner directing combustion products into the melting zone under a level of the molten material in the zone. Burners impart turbulence to the molten material in the melting zone. The fluid-cooled refractory panel is cooled, forming a modified panel having a frozen or highly viscous material layer on a surface of the panel facing the moltenmore » material, and a sensor senses temperature of the modified panel using a protected thermocouple positioned in the modified panel shielded from direct contact with turbulent molten material. Processes include controlling the melter using the temperature of the modified panel. Other processes and systems are presented.« less

Dueling Mechanisms for Dry Zones around Frozen Droplets

NASA Astrophysics Data System (ADS)

Bisbano, Caitlin; Nath, Saurabh; Boreyko, Jonathan

2016-11-01

Ice acts as a local humidity sink, due to its depressed saturation pressure relative to that of supercooled water. Hygroscopic chemicals typically exhibit annular dry zones of inhibited condensation; however, dry zones do not tend to form around ice because of inter-droplet frost growth to nearby liquid droplets that have already condensed on the chilled surface. Here, we use a humidity chamber with an embedded Peltier stage to initially suppress the growth of condensation on a chilled surface containing a single frozen droplet, in order to characterize the dry zone around ice for the first time. The length of the dry zone was observed to vary by at least two orders of magnitude as a function of surface temperature, ambient humidity, and the size of the frozen droplet. The surface temperature and ambient humidity govern the magnitudes of the in-plane and out-of-plane gradients in vapor pressure, while the size of the frozen droplet effects the local thickness of the concentration boundary layer. We develop an analytical model that reveals two different types of dry zones are possible: one in which nucleation is inhibited and one where the net growth of condensate is inhibited. Finally, a phase map was developed to predict the parameter space in which nucleation dry zones versus flux dry zones are dominant.

Accelerated retinal ganglion cell death in mice deficient in the Sigma-1 receptor.

PubMed

Mavlyutov, Timur A; Nickells, Robert W; Guo, Lian-Wang

2011-04-26

The sigma-1 receptor (σR1), a ligand-operated chaperone, has been inferred to be neuroprotective in previous studies using σR1 ligands. The σR1 specificity of the protective function, however, has yet to be firmly established, due to the existence of non-σR1 targets of the ligands. Here, we used the σR1-knockout mouse (Sigmar1(-/-)) to demonstrate unambiguously the role of the σR1 in protecting the retinal ganglion cells against degeneration after acute damage to the optic nerve. Retinal σR binding sites were labeled with radioiodinated σR ligands and analyzed by autoradiography. Localization of the σR1 was performed by indirect immunofluorescence on frozen retinal sections. Retinal ganglion cell death was induced by acute optic nerve crush in wild-type and Sigmar1(-/-) mice. Surviving cells in the ganglion cell layer were counted on Nissl-stained retinal whole mounts 7 days after the crush surgery. Photoaffinity labeling indicated the presence of the σR1 in the retina, in concentrations equivalent to those in liver tissue. Immunolabeling detected this receptor in cells of both the ganglion cell layer and the photoreceptor cell layer in wild-type retinas. Quantification of cells remaining after optic nerve crush showed that 86.8±7.9% cells remained in the wild-type ganglion cell layer, but only 68.3±3.4% survived in the Sigmar1(-/-), demonstrating a significant difference between the wild-type and the Sigmar1(-/-) in crush-induced ganglion cell loss. Our data indicated faster retinal ganglion cell death in Sigmar1(-/-) than in wild-type mice under the stresses caused by optic nerve crush, providing direct evidence for a role of the σR1 in alleviating retinal degeneration. This conclusion is consistent with the previous pharmacological studies using σR1 agonists. Thus, our study supports the idea that the σR1 is a promising therapeutic target for neurodegenerative retinal diseases, such as glaucoma.

Accelerated retinal ganglion cell death in mice deficient in the Sigma-1 receptor

PubMed Central

Mavlyutov, Timur A.; Nickells, Robert W.

2011-01-01

Purpose The sigma-1 receptor (σR1), a ligand-operated chaperone, has been inferred to be neuroprotective in previous studies using σR1 ligands. The σR1 specificity of the protective function, however, has yet to be firmly established, due to the existence of non-σR1 targets of the ligands. Here, we used the σR1-knockout mouse (Sigmar1−/−) to demonstrate unambiguously the role of the σR1 in protecting the retinal ganglion cells against degeneration after acute damage to the optic nerve. Methods Retinal σR binding sites were labeled with radioiodinated σR ligands and analyzed by autoradiography. Localization of the σR1 was performed by indirect immunofluorescence on frozen retinal sections. Retinal ganglion cell death was induced by acute optic nerve crush in wild-type and Sigmar1−/− mice. Surviving cells in the ganglion cell layer were counted on Nissl-stained retinal whole mounts 7 days after the crush surgery. Results Photoaffinity labeling indicated the presence of the σR1 in the retina, in concentrations equivalent to those in liver tissue. Immunolabeling detected this receptor in cells of both the ganglion cell layer and the photoreceptor cell layer in wild-type retinas. Quantification of cells remaining after optic nerve crush showed that 86.8±7.9% cells remained in the wild-type ganglion cell layer, but only 68.3±3.4% survived in the Sigmar1−/−, demonstrating a significant difference between the wild-type and the Sigmar1−/− in crush-induced ganglion cell loss. Conclusions Our data indicated faster retinal ganglion cell death in Sigmar1−/− than in wild-type mice under the stresses caused by optic nerve crush, providing direct evidence for a role of the σR1 in alleviating retinal degeneration. This conclusion is consistent with the previous pharmacological studies using σR1 agonists. Thus, our study supports the idea that the σR1 is a promising therapeutic target for neurodegenerative retinal diseases, such as glaucoma. PMID:21541278

Hydrated salt minerals on Ganymede's surface: evidence of an ocean below.

PubMed

McCord, T B; Hansen, G B; Hibbitts, C A

2001-05-25

Reflectance spectra from Galileo's near-infrared mapping spectrometer (NIMS) suggests that the surface of Ganymede, the largest satellite of Jupiter, contains hydrated materials. These materials are interpreted to be similar to those found on Europa, that is, mostly frozen magnesium sulfate brines that are derived from a subsurface briny layer of fluid.

In Vivo Efficacy of Fresh vs. Frozen Osteochondral Allografts in the Goat at 6 Months is Associated with PRG4 Secretion

PubMed Central

Pallante-Kichura, Andrea L.; Chen, Albert C.; Temple-Wong, Michele M.; Bugbee, William D.; Sah, Robert L.

2014-01-01

The long-term efficacy of osteochondral allografts is due to the presence of viable chondrocytes within graft cartilage. Chondrocytes in osteochondral allografts, especially those at the articular surface that normally produce the lubricant proteoglycan-4 (PRG4), are susceptible to storage-associated death. The hypothesis of this study was that the loss of chondrocytes within osteochondral grafts leads to decreased PRG4 secretion, after graft storage and subsequent implant. The objectives were to determine the effect of osteochondral allograft treatment (FROZEN vs. FRESH) on secretion of functional PRG4 after (i) storage, and (ii) 6months in vivo in adult goats. FROZEN allograft storage reduced PRG4 secretion from cartilage by ~85% compared to FRESH allograft storage. After 6months in vivo, the PRG4-secreting function of osteochondral allografts was diminished with prior FROZEN storage by ~81% versus FRESH allografts and by ~84% versus non-operated control cartilage. Concomitantly, cellularity at the articular surface in FROZEN allografts was ~96% lower than FRESH allografts and non-operated cartilage. Thus, the PRG4-secreting function of allografts appears to be maintained in vivo based on its state after storage. PRG4 secretion may be not only a useful marker of allograft performance, but also a biological process protecting the articular surface of grafts following cartilage repair. PMID:23362152

Personal cooling in nuclear power stations. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kamon, E.

1983-03-01

Two approaches to personal, non-restrictive cooling of workers exposed to high-temperature work environments in nuclear power plants were evaluated. Both approaches involved a cooling garment designed to be worn under the protective clothing donned in penetration into radiation areas. One garmet was developed to cool by direct body contact with small packets of frozen water enclosed in the pockets of a shirt. The other garmets cooled by circulating a cooled liquid through capillaries in a vest and head cap (System A) or a vest (System B). Testing was conducted in a laboratory simulation of high ambient temperature (55/sup 0/C) andmore » moderate metabolic heat production (200 to 300 kcal/h). Exposure time without cooling (control) was 52 minutes (Group 1) for the workloads demanding 200 kcal/h (48 minutes for Group 2). A long garmet with 7.2 kg of frozen water (LFWG) increased mean exposure time over the control by 242% (163% for the same garmet with 6.2 kg of frozen water). A short-version garmet with 3.8 kg of frozen water (SFWG) increased the stay time by 115%. The circulating-liquid garmets increased mean exposure time 35% (System A) and 27% (System B) over the control. In field observation, the LFWG with 6.2 kg of frozen water improved stay time by 125%.« less

Wave Runup on a Frozen Beach Under High Energy Conditions

NASA Astrophysics Data System (ADS)

Didier, D.; Bernatchez, P.; Dumont, D.; Corriveau, M.

2017-12-01

High and mid-latitude beaches have typical morphological characteristics influenced by nearshore processes prevailing under ice conditions during cold season. Nearshore ice complexes (NIC) offer a natural coastal protection by covering beach sediments, while offshore ice-infested waters dissipate incoming waves. Climate change contributes to sea ice shrinking therefore reducing its protection against erosion and flooding. In the Estuary and Gulf of the St. Lawrence (ESL, GSL) (eastern Canada), sea ice cover undergoes an overall shrinking and simulated future projections tend toward a negligible effect on wave climate by 2100. Quantifying the effect of nearshore dynamics on frozen beaches is therefore imperative for coastal management as more wave energy at the coast is expected in the future. To measure the effect of a frozen beach on wave runup elevations, this study employs a continuous video recording of the swash motion at 4Hz. Video-derived wave runup statistics have been extracted during a tidal cycle on a frozen beach, using the Pointe-Lebel beach (ESL) as a test case. Timestack analysis was combined with offshore water levels and wave measurements. A comparison of runup under icy conditions (Dec. 30 2016) with a runup distribution during summer was made under similar high energy wave conditions. Results indicate high runup excursions potentially caused by lowered sediment permeability due to high pore-ice saturation in the swash zone, accentuating the overwash of the eroding coastline and thus the risk of flooding. With projected reduction in coastal sea ice cover and thus higher wave energy, this study suggests that episodes of degradation and weakening could influence the coastal flood risk in mid- and high-latitude cold environments.

Protocols for dry DNA storage and shipment at room temperature

PubMed Central

Ivanova, Natalia V; Kuzmina, Masha L

2013-01-01

The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica® DNAstable® plates, home-made trehalose and polyvinyl alcohol (PVA) plates on 96-well panels of insect DNA stored at 56 °C and at room temperature. Controls included unprotected samples that were stored dry at room temperature and at 56 °C, and diluted samples held at 4 °C and at −20 °C. PCR and selective sequencing were performed over a 4-year interval to test the condition of DNA extracts. Biomatrica® provided better protection of DNA at 56 °C and at room temperature than trehalose and PVA, especially for diluted samples. PVA was the second best protectant after Biomatrica® at room temperature, whereas trehalose was the second best protectant at 56 °C. In spite of lower PCR success, the DNA stored at −20 °C yielded longer sequence reads and stronger signal, indicating that temperature is a crucial factor for DNA quality which has to be considered especially for long-term storage. Although it is premature to advocate a transition to DNA storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All three forms of DNA preservation enable shipment of dry DNA and PCR products between barcoding facilities. PMID:23789643

Detecting a liquid and solid H2O layer by geophysical methods

NASA Astrophysics Data System (ADS)

Yoshikawa, K.; Romanovsky, V.; Tsapin, A.; Brown, J.

2002-12-01

The objective is to detect the hydrological and cryological structure of the cold continuous permafrost subsurface using geophysical methods. We believe that a lot of water potentially exists as solid and liquid phases underground on Mars. It is likely that the liquid fluid would be high in saline concentration (brine). The ground freezing process involves many hydrological processes including enrichment of the brine layer. The brine layer is an important environment for ancient and/or current life to exist on terrestrial permafrost regions. The existence of a Martian brine layer would increase the possibility of the existence of life, as on Earth. In situ electric resistivity measurement will be the most efficient method to determine brine layer as well as massive H2O ice in the permafrost. However, the wiring configuration is unlikely to operate on the remote planetary surface. Satellite-born Radar and/or EM methods will be the most accessible methods for detecting the hydrological and cryological structure. We are testing several geophysical methods at the brine layer site in Barrow and massive pingo ice site in Fairbanks, Alaska. The radar system is affected by the dielectric properties of subsurface materials, which allows for evidence of liquid phase in the frozen ground. The dielectric constant varies greatly between liquid water and frozen ground. The depth of the terrestrial (and probably Martian) brine layer is frequently located deeper than the maximum detecting depth of the impulse type of the ground penetrating radar system. Once we develop a radar system with a deeper penetrating capability (Lower frequency), the dispersion of the ground ice will be the key function for interpretation of these signals. We will improve and use radar signals to understand the hydrological and cryological structure in the permafrost. The core samples and borehole temperature data validate these radar signals.

Flexible packaging for microelectronic devices

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Benjamin John; Nielson, Gregory N.; Cruz-Campa, Jose Luis

An apparatus, method, and system, the apparatus and system including a flexible microsystems enabled microelectronic device package including a microelectronic device positioned on a substrate; an encapsulation layer encapsulating the microelectronic device and the substrate; a protective layer positioned around the encapsulating layer; and a reinforcing layer coupled to the protective layer, wherein the substrate, encapsulation layer, protective layer and reinforcing layer form a flexible and optically transparent package around the microelectronic device. The method including encapsulating a microelectronic device positioned on a substrate within an encapsulation layer; sealing the encapsulated microelectronic device within a protective layer; and coupling themore » protective layer to a reinforcing layer, wherein the substrate, encapsulation layer, protective layer and reinforcing layer form a flexible and optically transparent package around the microelectronic device.« less

Simultaneous accumulation of proline and trehalose in industrial baker's yeast enhances fermentation ability in frozen dough.

PubMed

Sasano, Yu; Haitani, Yutaka; Hashida, Keisuke; Ohtsu, Iwao; Shima, Jun; Takagi, Hiroshi

2012-05-01

Freeze tolerance is a necessary characteristic for industrial baker's yeast because frozen-dough baking is one of the key technologies for supplying oven-fresh bakery products to consumers. Both proline and trehalose are known to function as cryoprotectants in yeast cells. In order to enhance the freeze tolerance of yeast cells, we constructed a self-cloning diploid baker's yeast strain with simultaneous accumulation of proline, by expressing the PRO1-I150T allele, encoding the proline-feedback inhibition-less sensitive γ-glutamyl kinase, and trehalose, by disrupting the NTH1 gene, encoding neutral trehalase. The resultant strain retained higher tolerance to oxidative and freezing stresses than did the single proline- or trehalose-accumulating strain. Interestingly, our results suggest that proline and trehalose protect yeast cells from short-term and long-term freezing, respectively. Simultaneous accumulation of proline and trehalose in industrial baker's yeast also enhanced the fermentation ability in the frozen dough compared with the single accumulation of proline or trehalose. These results indicate that baker's yeast that accumulates both proline and trehalose is applicable for frozen-dough baking. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Characterizations of low-temperature electroluminescence from ZnO nanowire light-emitting arrays on the p-GaN layer.

PubMed

Lu, Tzu-Chun; Ke, Min-Yung; Yang, Sheng-Chieh; Cheng, Yun-Wei; Chen, Liang-Yi; Lin, Guan-Jhong; Lu, Yu-Hsin; He, Jr-Hau; Kuo, Hao-Chung; Huang, JianJang

2010-12-15

Low-temperature electroluminescence from ZnO nanowire light-emitting arrays is reported. By inserting a thin MgO current blocking layer in between ZnO nanowire and p-GaN, high-purity UV light emission at wavelength 398 nm was obtained. As the temperature is decreased, contrary to the typical GaN-based light emitting diodes, our device shows a decrease of optical output intensity. The results are associated with various carrier tunneling processes and frozen MgO defects.

Chemical transformation of iodate(IO3-) and nitrite(NO2-) in frozen solution and its environmental implications.

NASA Astrophysics Data System (ADS)

Kim, Kitae

2017-04-01

Ice is ubiquitous on earth and involved in various chemical reactions in the environment. Most chemical reactions are slowed down when temperature decreases according to Arrhenius equation. However, several chemical processes can be enhanced in frozen state. Reactive halogen species play important roles in the global environment. In particular, the presence of gaseous halogens in the polar and marine boundary layers is of great interest because these highly reactive species can affect ozone and mercury depletion events, oxidizing capacity, and DMS(dimethylsulfide) oxidation to form cloud-condensation nuclei. Among halogen compounds, the sources and emission mechanisms of inorganic iodine species in the polar region remain unclear. Iodide(I-) and iodate(IO3-) are the most dominant iodine species in nature and their chemical transformation or I-/IO3- ratio in frozen state remains poorly understood. Recent study shows that the production of active iodine compounds from frozen iodate salts during photochemical reaction. In previous study, nitrite(NO2-) oxidation to nitrate(NO3-), which is very slow reaction in aqueous solution, was significantly (105 times) accelerated in frozen state. The enhanced proton and oxygen concentration within ice grain boundaries are suspected that the enhanced oxidation of nitrite by freezing. Here we investigate chemical transformation of iodate/nitrite mixture in ice. The results show that the chemical reaction between iodate and nitrite in ice can potentially provide a new pathway for the source of reactive iodine species to the polar atmosphere. The detailed experimental conditions and mechanism will be discussed in the presentation.

Sensitivity of Historical Simulation of the Permafrost to Different Atmospheric Forcing Data Sets from 1979 to 2009

NASA Astrophysics Data System (ADS)

Guo, Donglin; Wang, Huijun; Wang, Aihui

2017-11-01

Numerical simulation is of great importance to the investigation of changes in frozen ground on large spatial and long temporal scales. Previous studies have focused on the impacts of improvements in the model for the simulation of frozen ground. Here the sensitivities of permafrost simulation to different atmospheric forcing data sets are examined using the Community Land Model, version 4.5 (CLM4.5), in combination with three sets of newly developed and reanalysis-based atmospheric forcing data sets (NOAA Climate Forecast System Reanalysis (CFSR), European Centre for Medium-Range Weather Forecasts Re-Analysis Interim (ERA-I), and NASA Modern Era Retrospective-Analysis for Research and Applications (MERRA)). All three simulations were run from 1979 to 2009 at a resolution of 0.5° × 0.5° and validated with what is considered to be the best available permafrost observations (soil temperature, active layer thickness, and permafrost extent). Results show that the use of reanalysis-based atmospheric forcing data set reproduces the variations in soil temperature and active layer thickness but produces evident biases in their climatologies. Overall, the simulations based on the CFSR and ERA-I data sets give more reasonable results than the simulation based on the MERRA data set, particularly for the present-day permafrost extent and the change in active layer thickness. The three simulations produce ranges for the present-day climatology (permafrost area: 11.31-13.57 × 106 km2; active layer thickness: 1.10-1.26 m) and for recent changes (permafrost area: -5.8% to -9.0%; active layer thickness: 9.9%-20.2%). The differences in air temperature increase, snow depth, and permafrost thermal conditions in these simulations contribute to the differences in simulated results.

Near-Surface Profiles of Water Stable Isotope Components and Indicated Transitional History of Ice-Wedge Polygons Near Barrow

NASA Astrophysics Data System (ADS)

Iwahana, G.; Wilson, C.; Newman, B. D.; Heikoop, J. M.; Busey, R.

2017-12-01

Wetlands associated with ice-wedge polygons are commonly distributed across the Arctic Coastal Plain of northern Alaska, a region underlain by continuous permafrost. Micro-topography of the ice-wedge polygons controls local hydrology, and the micro-topography could be altered due to factors such like surface vegetation, wetness, freeze-thaw cycles, and permafrost degradation/aggradation under climate change. Understanding status of the wetlands in the near future is important because it determines biogeochemical cycle, which drives release of greenhouse gases from the ground. However, transitional regime of the ice-wedge polygons under the changing climate is not fully understood. In this study, we analyzed geochemistry of water extracted from frozen soil cores sampled down to about 1m depth in 2014 March at NGEE-Arctic sites in the Barrow Environmental Observatory. The cores were sampled from troughs/rims/centers of five different low-centered or flat-centered polygons. The frozen cores are divided into 5-10cm cores for each location, thawed in sealed plastic bags, and then extracted water was stored in vials. Comparison between the profiles of geochemistry indicated connection of soil water in the active layer at different location in a polygon, while it revealed that distinctly different water has been stored in permafrost layer at troughs/rims/centers of some polygons. Profiles of volumetric water content (VWC) showed clear signals of freeze-up desiccation in the middle of saturated active layers as low VWC anomalies at most sampling points. Water in the active layer and near-surface permafrost was classified into four categories: ice wedge / fresh meteoric / transitional / highly fractionated water. The overall results suggested prolonged separation of water in the active layer at the center of low-centered polygons without lateral connection in water path in the past.

State transitions and physicochemical aspects of cryoprotection and stabilization in freeze-drying of Lactobacillus rhamnosus GG (LGG).

PubMed

Pehkonen, K S; Roos, Y H; Miao, S; Ross, R P; Stanton, C

2008-06-01

The frozen and dehydrated state transitions of lactose and trehalose were determined and studied as factors affecting the stability of probiotic bacteria to understand physicochemical aspects of protection against freezing and dehydration of probiotic cultures. Lactobacillus rhamnosus GG was frozen (-22 or -43 degrees C), freeze-dried and stored under controlled water vapour pressure (0%, 11%, 23% and 33% relative vapour pressure) conditions. Lactose, trehalose and their mixture (1 : 1) were used as protective media. These systems were confirmed to exhibit relatively similar state transition and water plasticization behaviour in freeze-concentrated and dehydrated states as determined by differential scanning calorimetry. Ice formation and dehydrated materials were studied using cold-stage microscopy and scanning electron microscopy. Trehalose and lactose-trehalose gave the most effective protection of cell viability as observed from colony forming units after freezing, dehydration and storage. Enhanced cell viability was observed when the freezing temperature was -43 degrees C. State transitions of protective media affect ice formation and cell viability in freeze-drying and storage. Formation of a maximally freeze-concentrated matrix with entrapped microbial cells is essential in freezing prior to freeze-drying. Freeze-drying must retain a solid amorphous state of protectant matrices. Freeze-dried matrices contain cells entrapped in the protective matrices in the freezing process. The retention of viability during storage seems to be controlled by water plasticization of the protectant matrix and possibly interactions of water with the dehydrated cells. Highest cell viability was obtained in glassy protective media. This study shows that physicochemical properties of protective media affect the stability of dehydrated cultures. Trehalose and lactose may be used in combination, which is particularly important for the stabilization of probiotic bacteria in dairy systems.

Arctic Oases? - River Aufeis Maintain Perennial Groundwater Habitat in the Arctic

NASA Astrophysics Data System (ADS)

Huryn, A. D.; Gooseff, M. N.; Briggs, M. A.; Terry, N.; Kendrick, M.; Hendrickson, P. J.; Grunewald, E. D.

2017-12-01

Aufeis are massive accumulations of ice found along many arctic rivers, with aufeis on some Alaskan rivers covering 20+ km2 and attaining thicknesses of 5+ m. Although aufeis are prominent landscape features, understanding of their ecology is poor. We propose that aufeis function as summer oases by providing meltwater and nutrients to downstream habitats and winter oases due to insulating layers of ice that maintain below-ground freshwater habitat that would otherwise be frozen in regions of continuous permafrost. To gain information about the ecological roles of aufeis, we installed 50 1-m deep wells in a 1.5 km2aufeis field along the Kuparuk River, an arctic tundra river in Alaska. We assessed the predictions that aufeis provide perennially-unfrozen, below-ground habitat for a specialized invertebrate community while functioning as hot spots for nutrient regeneration. Surface geophysical measurements made during the time of maximum ice thickness in 2017 indicated most of the Kuparuk feature was underlain by several meters of shallow frozen cobbles that were further underlain by a 10 m thick+ zone of thawed material. Ground penetrating radar and surface nuclear magnetic resonance data indicated several spatially-discrete thawed preferential flow zones through the shallow frozen sediment layer that may result from groundwater upwelling observed flowing through fractures in the icepack. Freshwater invertebrates sampled from the wells represent a rich community consisting of flatworms, annelids, copepods, ostracods, chironomids (Krenosmittia, Trichotanypus) and stoneflies. Although the potential for below-ground NH4+ and NO3-regeneration was indicated, analyses were complicated bya surprisingly strong and dynamic coupling of surface and below-ground water.

Centrifugal accelerator, system and method for removing unwanted layers from a surface

DOEpatents

Foster, Christopher A.; Fisher, Paul W.

1995-01-01

A cryoblasting process having a centrifugal accelerator for accelerating frozen pellets of argon or carbon dioxide toward a target area utilizes an accelerator throw wheel designed to induce, during operation, the creation of a low-friction gas bearing within internal passages of the wheel which would otherwise retard acceleration of the pellets as they move through the passages. An associated system and method for removing paint from a surface with cryoblasting techniques involves the treating, such as a preheating, of the painted surface to soften the paint prior to the impacting of frozen pellets thereagainst to increase the rate of paint removal. A system and method for producing large quantities of frozen pellets from a liquid material, such as liquid argon or carbon dioxide, for use in a cryoblasting process utilizes a chamber into which the liquid material is introduced in the form of a jet which disintegrates into droplets. A non-condensible gas, such as inert helium or air, is injected into the chamber at a controlled rate so that the droplets freeze into bodies of relatively high density.

Nitrite-embedded packaging film effects on fresh and frozen beef color development and stability as influenced by meat age and muscle type.

PubMed

Claus, James R; Du, Chen

2013-11-01

Muscles (Longissimus lumborum, LL; Psoas major, PM, semitendinosus, ST) were aged (2, 9d postmortem), cut into steaks, anaerobically packaged (nitrite-embedded film, NEF), and displayed (fresh, 19d; frozen, 39d). Fresh NEF increased (P<0.05) in redness (first 48h). Upon opening fresh NEF (d 6) and overwrapping in PVC film, redness declined (P<0.05). NEF cooked LL had more red surface compared to non-NEF. Meat age influenced NEF color. Intact NEF maintained acceptable red color throughout display. Residual nitrite and nitrate associated with fresh NEF and nitrate in NEF cooked LL were found (P<0.05) in the outer layer. Consideration should be given to providing sufficient time for nitric oxide myoglobin development when using NEF which may be influenced by meat age and muscle differences. NEF packaging has potential to extend fresh beef color display life. NEF appears to offer the opportunity to display bright red beef in frozen display by limiting typical effects of photooxidation. Copyright © 2013 Elsevier Ltd. All rights reserved.

Effects of arginine and trehalose on post-thawed bovine sperm quality.

PubMed

Öztürk, Caner; Güngör, Şükrü; Ataman, Mehmet Bozkurt; Bucak, Mustafa Numan; Başpinar, Nuri; Ili, Pınar; Inanç, Muhammed Enes

2017-09-01

The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 ± 8.21%), CASA motility (12.2 ± 5.69%) and progressive motility (3.52 ± 2.13%), compared with the controls (43 ± 2.73%, 55.4 ± 6.78% and 33.48 ± 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 ± 3.99% and 44.1 ± 2.18%) compared with the control (13 ± 8.15 and 31.7 ± 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.

Matched case-control phase 2 study to evaluate the use of a frozen sock to prevent docetaxel-induced onycholysis and cutaneous toxicity of the foot.

PubMed

Scotté, Florian; Banu, Eugeniu; Medioni, Jacques; Levy, Eric; Ebenezer, Christelle; Marsan, Sandrine; Banu, Adela; Tourani, Jean Marc; Andrieu, Jean-Marie; Oudard, Stéphane

2008-04-01

Onycholysis occurs in approximately 30% of patients treated with docetaxel. The efficacy and safety of an Elasto-Gel frozen sock (FS) was investigated for the prevention of docetaxel-induced nail and skin toxicity of the feet. Patients receiving docetaxel at a dose of 70 to 100 mg/m(2) every 3 weeks were eligible for this matched case-control study. Each patient wore an FS for 90 minutes on the right foot. The unprotected left foot acted as control. Nail and skin toxicities were assessed using National Cancer Institute Common Toxicity Criteria (version 3) and compared using a 2-sample Wilcoxon matched-pairs rank test adjusted for tied values. Fifty consecutive patients were included between April 2005 and January 2007. Nail toxicity was significantly lower in the FS-protected foot compared with the control foot (grade 0: 100% versus 79%; and grade 1 and 2: 0% versus 21%, respectively) (P= .002). Skin toxicity was grade 0: 98% versus 94%; and grade 1 and 2: 2% versus 6% in the FS-protected and the control feet, respectively. The median times until toxicity occurrence were not found to differ significantly between the groups. One patient experienced discomfort because of cold intolerance. Cold therapy using FS significantly reduced the incidence of docetaxel-induced foot nail toxicity, as previously demonstrated using frozen gloves for the hands.

Effects of different extenders on DNA integrity of boar spermatozoa following freezing-thawing.

PubMed

Hu, Jian-hong; Li, Qing-wang; Jiang, Zhong-liang; Li, Wen-ye

2008-12-01

The sperm-rich fraction, collected from eight mature Yorkshire boars, was frozen in an extender containing 9% LDL (w/v), 100mM trehalose, or 20% yolk (v/v), respectively. Sperm DNA integrity was assessed using the single-cell gel electrophoresis (SCGE). Other sperm quality characteristics such as motility, acrosome and membrane integrity were also monitored. The results showed that freezing-thawing caused an increase in sperm DNA fragmentation, and extender containing 9% LDL could significantly protect sperm DNA integrity (P<0.05) from the damage caused by cryopreservation and decrease DNA damages compared with extender containing 100mM trehalose and 20% yolk (v/v). No significant difference in damaged DNA was detected between frozen and unfrozen semen samples for extender of 9% LDL and 100mM trehalose, but cryopreservation could increase the degree of DNA damage (P<0.05), the percentage of damaged DNA degree of grade 2 and 3 was significantly increased. The deterioration in post-thaw sperm DNA integrity was concurrent with reduced sperm characteristics. The data here demonstrated that the cryoprotectant played a fundamental role in reducing boar sperm DNA damage and protecting DNA integrity. It can be suggested that evaluation of sperm DNA integrity, coupled with correlative and basic characteristics such as motility, acrosome integrity and membrane integrity, may aid in determining the quality of frozen boar semen.

14 CFR Appendix G to Part 417 - Natural and Triggered Lightning Flight Commit Criteria

Code of Federal Regulations, 2010 CFR

2010-01-01

... time. A cumulus cloud formed locally and a cirrus layer that is physically separated from that cumulus... launch point at the same time. Bright band means an enhancement of radar reflectivity caused by frozen.... Cloud means a visible mass of water droplets or ice crystals produced by condensation of water vapor in...

14 CFR Appendix G to Part 417 - Natural and Triggered Lightning Flight Commit Criteria

Code of Federal Regulations, 2011 CFR

2011-01-01

... time. A cumulus cloud formed locally and a cirrus layer that is physically separated from that cumulus... launch point at the same time. Bright band means an enhancement of radar reflectivity caused by frozen.... Cloud means a visible mass of water droplets or ice crystals produced by condensation of water vapor in...

THE ACTION OF EXTREME COLD ON LEUKEMIC CELLS OF MICE

PubMed Central

Breedis, Charles

1942-01-01

Suspensions of leukemic cells of mice from three different strains of leukemia were subjected to rapid or slow freezing and rapid or slow thawing. Suspensions rapidly frozen to –196°C. were in all cases innocuous, whereas those frozen slowly were capable of transmitting leukemia. The infectivity of slowly frozen material varied from an estimated 0.0001 per cent to 1 per cent of that of fresh material, and this figure probably represents the percentage of surviving leukemic cells. Particles of spleen and lymph node reacted to slow and rapid freezing in the same manner as suspensions prepared from them. For one of the strains rapid thawing was less injurious than slow thawing; for the other two the rate of thawing seemed to be immaterial. Infectivity was equally well preserved after freezing to –21°C. whether freezing occurred spontaneously after supercooling or was initiated near the freezing point by inoculation with ice, or whether thawing was slow or rapid. Suspensions already slowly frozen at temperatures of –2° or lower, whether spontaneously or by inoculation with ice, could no longer be completely inactivated by subsequent rapid cooling to –196°C. Unfrozen suspensions initially above the freezing point or supercooled to –2°C. or –8°C. and then rapidly cooled to –196°C. were inactivated. This protective action of previous slow freezing was most marked when the initial temperature of the frozen suspension was –15°C. or lower; when it was –2°C. protection was barely detected. These observations indicate that the changes which are peculiar to rapid freezing alone and lead to complete inactivation take place during rapid transition from the liquid to the solid state, in a range of temperature lying between –15°C. and the freezing point. Temperature measurements carried out in this range showed that suspensions were about equally infections whether the temperature at their centers dropped from 0°C. to –15°C. in 30 minutes or in 1 minute; when the drop occurred in 12 seconds or less, the suspensions became innocuous. PMID:19871231

Biologically active antimicrobial and antioxidant substances in the Helianthus annuus L. bee pollen.

PubMed

Fatrcová-Šramková, Katarína; Nôžková, Janka; Máriássyová, Magda; Kačániová, Miroslava

2016-01-01

The objective of this study was to measure the content of flavonoids, polyphenols, and carotenoids in the Helianthus annuus L. bee pollen. It was also to evaluate the ability of the dried, frozen, and freeze-dried extracts of sunflower (H. annuus) pollen, its scavenged free radicals and reducing action. Another aim of this study was to investigate the antimicrobial in vitro action of the H. annuus pollen extracts against the Gram-positive and Gram-negative bacteria and fungi. All pollen extracts showed medium antiradical activity and reductive ability. The most effective was the freeze-dried extract in both evaluation systems. The evaluation of the protective effects of DNA using a biosensor showed an opposite trending-frozen ˃ dried ˃ freeze-dried pollen. For the evaluation of antiradical activity, the DPPH method was used, and reductive ability was assessed by means of phosphomolybdic complex formation. The comparison of the polyphenols content shows higher values in freeze-dried bee pollen than in the dried and frozen pollen. The highest content of flavonoids was found in the frozen samples and the most carotenoids were present in the dried samples. In our study, the best antibacterial effects of the dried sunflower bee pollen extracts were found against Paenibacillus larvae, Pseudomonas aeruginosa, and Enterococcus raffinosus. The best inhibitory properties of the frozen sunflower bee pollen extracts were found against Escherichia coli, Pseudomonas aeruginosa, and Paenibacillus larvae. Very good inhibitory effects of freeze-dried sunflower bee pollen were found against Paenibacillus larvae, Brochotrix thermosphacta, and Enterococcus raffinosus. The best antifungal activity of the sunflower bee pollen was found in the frozen bee pollen extracts against Aspergillus ochraceus and freeze-dried bee pollen extracts against Aspergillus niger.

Preparation of a Frozen Regolith Simulant Bed for ISRU Component Testing in a Vacuum Chamber

NASA Technical Reports Server (NTRS)

Klenhenz, Julie; Linne, Diane

2013-01-01

In-Situ Resource Utilization (ISRU) systems and components have undergone extensive laboratory and field tests to expose hardware to relevant soil environments. The next step is to combine these soil environments with relevant pressure and temperature conditions. Previous testing has demonstrated how to incorporate large bins of unconsolidated lunar regolith into sufficiently sized vacuum chambers. In order to create appropriate depth dependent soil characteristics that are needed to test drilling operations for the lunar surface, the regolith simulant bed must by properly compacted and frozen. While small cryogenic simulant beds have been created for laboratory tests, this scale effort will allow testing of a full 1m drill which has been developed for a potential lunar prospector mission. Compacted bulk densities were measured at various moisture contents for GRC-3 and Chenobi regolith simulants. Vibrational compaction methods were compared with the previously used hammer compaction, or "Proctor", method. All testing was done per ASTM standard methods. A full 6.13 m3 simulant bed with 6 percent moisture by weight was prepared, compacted in layers, and frozen in a commercial freezer. Temperature and desiccation data was collected to determine logistics for preparation and transport of the simulant bed for thermal vacuum testing. Once in the vacuum facility, the simulant bed will be cryogenically frozen with liquid nitrogen. These cryogenic vacuum tests are underway, but results will not be included in this manuscript.

Biopolymers for Sample Collection, Protection, and Preservation

DTIC Science & Technology

2015-05-19

biopolymer. The cured polymer results in a solid protective film that is stable to many organic solvents, but quickly removed by the application of the water...the biopolymer. The cured polymer results in a solid protective film that is stable to many organic solvents, but quickly removed by the appli- cation...frozen after that. RNAlater® has been demonstrated to Ta bl e 1 C om pa ri so n of ce ll an d nu cl ei c ac id pr es er va tio n te ch ni qu es Sy st

Effects of retail style or food service style packaging type and storage time on sensory characteristics of bacon manufactured from commercially sourced pork bellies.

PubMed

Lowe, B K; Bohrer, B M; Holmer, S F; Boler, D D; Dilger, A C

2014-06-01

Objectives were to characterize differences in pork bellies that were stored frozen for different durations prior to processing and characterize sensory properties of the bacon derived from those bellies when stored in either retail or food service style packaging. Bellies (n = 102) were collected from 4 different time periods, fresh bellies (never frozen) and bellies frozen for 2, 5, or 7 mo, and manufactured into bacon under commercial conditions. Food service bacon was packaged in oxygen-permeable polyvinyl lined boxes layered on wax-covered lined paper and blast frozen (-33 °C) for 45 or 90 d after slicing. Retail bacon was vacuum-packaged in retail packages and refrigerated (2 °C) in the dark for 60 or 120 d after slicing. At the end of respective storage times after slicing, bacon was analyzed for sensory attributes and lipid oxidation. Off-flavor and oxidized odor of bacon increased (P < 0.01) with increasing storage time in both packaging types. Lipid oxidation increased (P < 0.01) as storage time increased from day 0 to day 45 in food service packaged bacon from frozen bellies, but was unchanged (P ≥ 0.07) with time in food service packaged bacon from fresh bellies. Lipid oxidation was also unchanged (P ≥ 0.21) over time in retail packaged bacon, with the exception of bellies frozen for 5 mo, which was increased from day 0 to day 90. Overall, off-flavor, oxidized odor, and lipid oxidation increased as storage time after processing increased. Freezing bellies before processing may exacerbate lipid oxidation as storage time after processing was extended. Bacon can be packaged and managed several different ways before it reaches the consumer. This research simulated food service (frozen) and retail packaged (refrigerated) bacon over a range of storage times after slicing. Off-flavor and oxidized odor increased as storage time after processing increased in both packaging types. Lipid oxidation increased as storage time after slicing increased to a greater extent in food service packaging. © 2014 Institute of Food Technologists®

Colloid centrifugation of fresh stallion semen before cryopreservation decreased microorganism load of frozen-thawed semen without affecting seminal kinetics.

PubMed

Guimarães, T; Lopes, G; Pinto, M; Silva, E; Miranda, C; Correia, M J; Damásio, L; Thompson, G; Rocha, A

2015-01-15

Freezability of equine semen may be influenced by microorganism population of semen. The objective of this study was to verify the effect of single-layer density gradient centrifugation (SLC) of fresh semen before cryopreservation on semen's microbial load (ML) and sperm cells kinetics after freezing-thawing. For that, one ejaculate was collected from 20 healthy stallions and split into control (C) samples (cryopreserved without previous SLC) and SLC samples (subjected to SLC). Semen cryopreservation was performed according to the same protocol in both groups. Microbial load of each microorganism species and total microbial load (TML) expressed in colony-forming units (CFU/mL) as well as frozen-thawed sperm kinetics were assessed in both groups. Additional analysis of the TML was performed, subdividing the frozen-thawed samples in "suitable" (total motility ≥ 30%) and "unsuitable" (total motility < 30%) semen for freezing programs, and comparing the C and SLC groups within these subpopulations. After thawing, SLC samples had less (P < 0.05) TML (88.65 × 10(2) ± 83.8 × 10(2) CFU/mL) than C samples (155.69 × 10(2) ± 48.85 × 10(2) CFU/mL), mainly due to a reduction of Enterococcus spp. and Bacillus spp. A relationship between post-thaw motility and SLC effect on ML was noted, as only in samples with more than 30% total motility was ML reduced (P < 0.05) by SLC (from 51.33 × 10(2) ± 33.26 × 10(2) CFU/mL to 26.68 × 10(2) ± 12.39 × 10(2) CFU/mL in "suitable" frozen-thawed semen vs. 240.90 × 10(2) ± 498.20 × 10(2) to 139.30 × 10(2) ± 290.30 × 10(2) CFU/mL in "unsuitable" frozen-thawed semen). The effect of SLC on kinetics of frozen-thawed sperm cells was negligible. Copyright © 2015 Elsevier Inc. All rights reserved.

A SIMPLE FREEZE-FRACTURE REPLICATION METHOD FOR ELECTRON MICROSCOPY

PubMed Central

Bullivant, Stanley; Ames, Adelbert

1966-01-01

A simple method to achieve results similar to the freeze-etching technique of Moor et al. (1961) is described. The frozen tissue is cut under liquid nitrogen with a razor blade outside the evaporator rather than inside with a cooled microtome. The conditions of the experiment do not favor sublimation, and it is proposed that the structure of the replica be explained by local faults in the cleavage plane which leaves structures, such as membranes, standing above the ice. Micrographs of replicas of glycerol-protected frozen small intestine of mouse prepared by the method are presented and the structural details they show are discussed. The problem of vapor-deposited contamination is discussed. It is concluded that this is a practical method for obtaining electron micrographs that are relatively free of artifact, and that further improvements may be expected from the use of rapidly frozen fresh tissue and a clean vacuum system, possibly of the ion-pumped type. PMID:5962938

Systematic study on dynamic atomic layer epitaxy of InN on/in +c-GaN matrix and fabrication of fine-structure InN/GaN quantum wells: Role of high growth temperature

NASA Astrophysics Data System (ADS)

Yoshikawa, Akihiko; Kusakabe, Kazuhide; Hashimoto, Naoki; Hwang, Eun-Sook; Imai, Daichi; Itoi, Takaomi

2016-12-01

The growth kinetics and properties of nominally 1-ML (monolayer)-thick InN wells on/in +c-GaN matrix fabricated using dynamic atomic layer epitaxy (D-ALEp) by plasma-assisted molecular beam epitaxy were systematically studied, with particular attention given to the effects of growth temperature. Attention was also given to how and where the ˜1-ML-thick InN layers were frozen or embedded on/in the +c-GaN matrix. The D-ALEp of InN on GaN was a two-stage process; in the 1st stage, an "In+N" bilayer/monolayer was formed on the GaN surface, while in the 2nd, this was capped by a GaN barrier layer. Each process was monitored in-situ using spectroscopic ellipsometry. The target growth temperature was above 620 °C and much higher than the upper critical epitaxy temperature of InN (˜500 °C). The "In+N" bilayer/monolayer tended to be an incommensurate phase, and the growth of InN layers was possible only when they were capped with a GaN layer. The InN layers could be coherently inserted into the GaN matrix under self-organizing and self-limiting epitaxy modes. The growth temperature was the most dominant growth parameter on both the growth process and the structure of the InN layers. Reflecting the inherent growth behavior of D-ALEp grown InN on/in +c-GaN at high growth temperature, the embedded InN layers in the GaN matrix were basically not full-ML in coverage, and the thickness of sheet-island-like InN layers was essentially either 1-ML or 2-ML. It was found that these InN layers tended to be frozen at the step edges on the GaN and around screw-type threading dislocations. The InN wells formed type-I band line-up heterostructures with GaN barriers, with exciton localization energies of about 300 and 500 meV at 15 K for the 1-ML and 2-ML InN wells, respectively.

Lea's Pies

NASA Technical Reports Server (NTRS)

1996-01-01

The Technology Transfer Office at Stennis Space Center worked with a pie company owner to develop an inexpensive container that would protect pies and keep them in a near frozen condition for shipping in 48 hours. A NASA engineer made a thermal barrier envelope from a metalized mylar called 'space blanket material,' developed during the Apollo era. The envelope protects the pies from heat transfer. Pictured, a NASA engineer removes the temperature logger from a pecan pie shipped to him in a prototype envelope.

Protocols for dry DNA storage and shipment at room temperature.

PubMed

Ivanova, Natalia V; Kuzmina, Masha L

2013-09-01

The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica(®) DNAstable(®) plates, home-made trehalose and polyvinyl alcohol (PVA) plates on 96-well panels of insect DNA stored at 56 °C and at room temperature. Controls included unprotected samples that were stored dry at room temperature and at 56 °C, and diluted samples held at 4 °C and at -20 °C. PCR and selective sequencing were performed over a 4-year interval to test the condition of DNA extracts. Biomatrica(®) provided better protection of DNA at 56 °C and at room temperature than trehalose and PVA, especially for diluted samples. PVA was the second best protectant after Biomatrica(®) at room temperature, whereas trehalose was the second best protectant at 56 °C. In spite of lower PCR success, the DNA stored at -20 °C yielded longer sequence reads and stronger signal, indicating that temperature is a crucial factor for DNA quality which has to be considered especially for long-term storage. Although it is premature to advocate a transition to DNA storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All three forms of DNA preservation enable shipment of dry DNA and PCR products between barcoding facilities. © 2013 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.

75 FR 60817 - Non-Frozen Concentrated Apple Juice From China

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-01

... changes, if any, in the supply and demand conditions or business cycle for the Domestic Like Product that... disclosure of business proprietary information (BPI) under an administrative protective order (APO) and APO..., a U.S. or foreign trade or business association, or another interested party (including an...

Comparison of different histological protocols for the preservation and quantification of the intestinal mucus layer in pigs.

PubMed

Röhe, Ilen; Hüttner, Friedrich Joseph; Plendl, Johanna; Drewes, Barbara; Zentek, Jürgen

2018-02-05

The histological characterization of the intestinal mucus layer is important for many scientific experiments investigating the interaction between intestinal microbiota, mucosal immune response and intestinal mucus production. The aim of this study was to examine and compare different fixation protocols for displaying and quantifying the intestinal mucus layer in piglets and to test which histomorphological parameters may correlate with the determined mucus layer thickness. Jejunal and colonal tissue samples of weaned piglets (n=10) were either frozen in liquid nitrogen or chemically fixed using methacarn solution. The frozen tissue samples were cryosectioned and subsequently postfixed using three different postfixatives: paraformaldehyde vapor, neutrally buffered formalin solution and ethanol solution. After dehydration, methacarn fixed tissues were embedded in paraffin wax. Both sections of cryopreserved and methacarn fixed tissue samples were stained with Alcian blue (AB)-PAS followed by the microscopically determination of the mucus layer thickness. Different pH values of the Alcian Blue staining solution and two mucus layer thickness measuring methods were compared. In addition, various histomorphological parameters of methacarn fixed tissue samples were evaluated including the number of goblet cells and the mucin staining area. Cryopreservation in combination with chemical postfixation led to mucus preservation in the colon of piglets allowing mucus thickness measurements. Mucus could be only partly preserved in cryosections of the jejunum impeding any quantitative description of the mucus layer thickness. The application of different postfixations, varying pH values of the AB solution and different mucus layer measuring methods led to comparable results regarding the mucus layer thickness. Methacarn fixation proved to be unsuitable for mucus depiction as only mucus patches were found in the jejunum or a detachment of the mucus layer from the epithelium was observed in the colon. Correlation analyses revealed that the proportion of the mucin staining area per crypt area (relative mucin staining) measured in methacarn fixed tissue samples corresponded to the colonal mucus layer thickness determined in cryopreserved tissue samples. In conclusion, the results showed that cryopreservation using liquid nitrogen followed by chemical postfixation and AB-PAS staining led to a reliable mucus preservation allowing a mucus thickness determination in the colon of pigs. Moreover, the detected relative mucin staining area may serve as a suitable histomorphological parameter for the assessment of the intestinal mucus layer thickness. The findings obtained in this study can be used for the implementation of an improved standard for the histological description of the mucus layer in the colon of pigs.

Formation mechanism of the protective layer in a blast furnace hearth

NASA Astrophysics Data System (ADS)

Jiao, Ke-xin; Zhang, Jian-liang; Liu, Zheng-jian; Xu, Meng; Liu, Feng

2015-10-01

A variety of techniques, such as chemical analysis, scanning electron microscopy-energy dispersive spectroscopy, and X-ray diffraction, were applied to characterize the adhesion protective layer formed below the blast furnace taphole level when a certain amount of titanium- bearing burden was used. Samples of the protective layer were extracted to identify the chemical composition, phase assemblage, and distribution. Furthermore, the formation mechanism of the protective layer was determined after clarifying the source of each component. Finally, a technical strategy was proposed for achieving a stable protective layer in the hearth. The results show that the protective layer mainly exists in a bilayer form in the sidewall, namely, a titanium-bearing layer and a graphite layer. Both the layers contain the slag phase whose major crystalline phase is magnesium melilite (Ca2MgSi2O7) and the main source of the slag phase is coke ash. It is clearly determined that solid particles such as graphite, Ti(C,N) and MgAl2O4 play an important role in the formation of the protective layer, and the key factor for promoting the formation of a stable protective layer is reasonable control of the evolution behavior of coke.

Ice-dynamical and Basal Properties of Sofiyskiy Glacier, Altai Mts, Russia, Based On GPS and Radio-echo Sounding Surveys

NASA Astrophysics Data System (ADS)

Pattyn, F.; de Smedt, B.; de Brabander, S.; Decleir, H.

It is expected that central-Asian glaciers ­ compared to other glaciers in the world ­ exhibit a different response to changing climate due to (i) the extreme continentality that gives rise to aridity and large seasonal temperature variations, and (ii) the coinci- dence of both accumulation and ablation season in summer. A detailed four-year field survey was carried out on Sofiyskiy Glacier, a glacier in the South Chuya Range (Altai Mountains), Russia. Field observations revealed that this glacier retreated steadily during the 20th Century at a rate of 18.3 m a-1. Based on radio-echo sounding (RES) and mass-balance mea- surements, we carried out numerical model experiments to simulate historic front vari- ations (De Smedt and Pattyn, in prep.). Results demonstrated that although Sofiyskiy Glacier exhibits a low static sensitivity to climatic changes, its dynamic sensitivity seems very large compared to glaciers from the European Alps. Basal sliding plays an important role in the glacier's behaviour. An analysis of RES measurements led to the determination of the bed reflection power (BRP). Applying a three-layer reflectivity model (Born and Wolf, 1986) the BRP was compared with the theoretical bed reflectivity for a conductive layer of varying porosity and thickness (considering a water layer, saturated till or frozen material). From this comparison it was possible to delineate zones of high basal water content, saturated till or ice frozen to the bedrock.

Martian "Swiss Cheese"

NASA Image and Video Library

2000-04-24

This image is illuminated by sunlight from the upper left. Looking like pieces of sliced and broken swiss cheese, the upper layer of the martian south polar residual cap has been eroded, leaving flat-topped mesas into which are set circular depressions such as those shown here. The circular features are depressions, not hills. The largest mesas here stand about 4 meters (13 feet) high and may be composed of frozen carbon dioxide and/or water. Nothing like this has ever been seen anywhere on Mars except within the south polar cap, leading to some speculation that these landforms may have something to do with the carbon dioxide thought to be frozen in the south polar region. On Earth, we know frozen carbon dioxide as "dry ice." On Mars, as this picture might be suggesting, there may be entire landforms larger than a small town and taller than 2 to 3 men and women that consist, in part, of dry ice. No one knows for certain whether frozen carbon dioxide has played a role in the creation of the "swiss cheese" and other bizarre landforms seen in this picture. The picture covers an area 3 x 9 kilometers (1.9 x 5.6 miles) near 85.6°S, 74.4°W at a resolution of 7.3 meters (24 feet) per pixel. This picture was taken by the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) during early southern spring on August 3, 1999. http://photojournal.jpl.nasa.gov/catalog/PIA02367

Production of Molecular Iodine and Tri-iodide in the Frozen Solution of Iodide: Implication for Polar Atmosphere.

PubMed

Kim, Kitae; Yabushita, Akihiro; Okumura, Masanori; Saiz-Lopez, Alfonso; Cuevas, Carlos A; Blaszczak-Boxe, Christopher S; Min, Dae Wi; Yoon, Ho-Il; Choi, Wonyong

2016-02-02

The chemistry of reactive halogens in the polar atmosphere plays important roles in ozone and mercury depletion events, oxidizing capacity, and dimethylsulfide oxidation to form cloud-condensation nuclei. Among halogen species, the sources and emission mechanisms of inorganic iodine compounds in the polar boundary layer remain unknown. Here, we demonstrate that the production of tri-iodide (I3(-)) via iodide oxidation, which is negligible in aqueous solution, is significantly accelerated in frozen solution, both in the presence and the absence of solar irradiation. Field experiments carried out in the Antarctic region (King George Island, 62°13'S, 58°47'W) also showed that the generation of tri-iodide via solar photo-oxidation was enhanced when iodide was added to various ice media. The emission of gaseous I2 from the irradiated frozen solution of iodide to the gas phase was detected by using cavity ring-down spectroscopy, which was observed both in the frozen state at 253 K and after thawing the ice at 298 K. The accelerated (photo-)oxidation of iodide and the subsequent formation of tri-iodide and I2 in ice appear to be related with the freeze concentration of iodide and dissolved O2 trapped in the ice crystal grain boundaries. We propose that an accelerated abiotic transformation of iodide to gaseous I2 in ice media provides a previously unrecognized formation pathway of active iodine species in the polar atmosphere.

Degradation and stabilization of ice wedges: Implications for assessing risk of thermokarst in northern Alaska

NASA Astrophysics Data System (ADS)

Kanevskiy, Mikhail; Shur, Yuri; Jorgenson, Torre; Brown, Dana R. N.; Moskalenko, Nataliya; Brown, Jerry; Walker, Donald A.; Raynolds, Martha K.; Buchhorn, Marcel

2017-11-01

Widespread degradation of ice wedges has been observed during the last decades in numerous areas within the continuous permafrost zone of Eurasia and North America. To study ice-wedge degradation, we performed field investigations at Prudhoe Bay and Barrow in northern Alaska during 2011-2016. In each study area, a 250-m transect was established with plots representing different stages of ice-wedge degradation/stabilization. Field work included surveying ground- and water-surface elevations, thaw-depth measurements, permafrost coring, vegetation sampling, and ground-based LiDAR scanning. We described cryostratigraphy of frozen soils and stable isotope composition, analyzed environmental characteristics associated with ice-wedge degradation and stabilization, evaluated the vulnerability and resilience of ice wedges to climate change and disturbances, and developed new conceptual models of ice-wedge dynamics that identify the main factors affecting ice-wedge degradation and stabilization and the main stages of this quasi-cyclic process. We found significant differences in the patterns of ice-wedge degradation and stabilization between the two areas, and the patterns were more complex than those previously described because of the interactions of changing topography, water redistribution, and vegetation/soil responses that can interrupt or reinforce degradation. Degradation of ice wedges is usually triggered by an increase in the active-layer thickness during exceptionally warm and wet summers or as a result of flooding or disturbance. Vulnerability of ice wedges to thermokarst is controlled by the thickness of the intermediate layer of the upper permafrost, which overlies ice wedges and protects them from thawing. In the continuous permafrost zone, degradation of ice wedges rarely leads to their complete melting; and in most cases wedges eventually stabilize and can then resume growing, indicating a somewhat cyclic and reversible process. Stabilization of ice wedges after their partial degradation makes them better protected than before degradation because the intermediate layer is usually 2 to 3 times thicker on top of stabilized ice wedges than on top of initial ice wedges in undisturbed conditions. As a result, the likelihood of formation of large thaw lakes in the continuous permafrost zone triggered by ice-wedge degradation alone is very low.

Data analysis and hydrological modelling of frozen ground, shallow groundwater formation and river flow co-evolution at small watersheds of Russia in continuous, discontinuous permafrost and the zone of seasonal ground freezing

NASA Astrophysics Data System (ADS)

Lebedeva, Luidmila; Semenova, Olga

2015-04-01

Frozen ground distribution and its properties control the presence of aquifuge and aquifers. Correct representation of interactions between infiltrating water, ground ice, permafrost or seasonal freezing table and river flow is challenging for hydrological modelling in cold regions. Observational data of ground water levels, thawing depths in different landscapes or topographical units and meteorological information with high temporal and spatial resolution are required to analyze seasonal and interannual evolution of groundwater in active layer and its linkage to river flow. Such data are extremely rare in vast and remote regions of Russia. There are few historical datasets inherited from former USSR containing unique collection of long-term daily observations of water fluxes, frozen ground characteristics and groundwater levels. The data from three water balance stations were employed in our study with overall goal to analyze co-evolution of thawing layer, shallow groundwater and river flow by data processing and process-based modelling. Three instrumented small watersheds are situated in continuous, discontinuous permafrost zones and at the territory with seasonally frozen ground. They present different climates, landscapes and geology. The Kolyma water-balance station is located in mountainous region of continuous permafrost in North-Eastern Russia. The watershed area of 22 km2 is covered by bare rocks, mountain tundra, sparse larch forest and wet larch forest depending on slope aspect and inclination. The Bomnak water-balance station (22 km2) is situated in discontinuous permafrost zone in upper part of the Amur River basin and characterized by unmerged permafrost. Dominant landscapes are birch forest and bogs. The Pribaltiyskaya water-balance station (40 km2) located in Latvia is characterized by seasonally frozen ground and is covered by mixed forest and arable land. Process-based Hydrograph model was employed in the study. The model was developed specifically for cold regions. It describes all essential processes of land hydrological cycle including detailed algorithm of water and heat dynamics in soil accounting for water phase change. The model parameters relate to basin characteristics and could be assessed in the field. It allows avoiding parameters calibration and transferring model parameterization schemes to ungauged basins in similar conditions. The model was applied and tested against internal states of watersheds (snow, soil thawing/freezing, etc.) and runoff. Different role of frozen ground in formation of shallow groundwater and river flow in continuous, discontinuous and non-permafrost area is highlighted by comparative analysis of observations and simulations in three studied basins. The changes of fractional input of surface and subsurface components into river flow during warm seasons were assessed for each watershed. We concluded that verified hydrological model with meaningful parameters that adequately describe river flow formation and internal hydrological processes and ground freezing/thawing in the catchment could be used in scenario simulations, future predictions and transferring the results between scales.

Rime and graupel: Description and characterization as revealed by low-temperature scanning electron microscopy

USGS Publications Warehouse

Rango, A.; Foster, J.; Josberger, E.G.; Erbe, E.F.; Pooley, C.; Wergin, W.P.

2003-01-01

Snow crystals, which form by vapor deposition, occasionally come in contact with supercooled cloud droplets during their formation and descent. When this occurs, the droplets adhere and freeze to the snow crystals in a process known as accretion. During the early stages of accretion, discrete snow crystals exhibiting frozen cloud droplets are referred to as rime. If this process continues, the snow crystal may become completely engulfed in frozen cloud droplets. The resulting particle is known as graupel. Light microscopic investigations have studied rime and graupel for nearly 100 years. However, the limiting resolution and depth of field associated with the light microscope have prevented detailed descriptions of the microscopic cloud droplets and the three-dimensional topography of the rime and graupel particles. This study uses low-temperature scanning electron microscopy to characterize the frozen precipitates that are commonly known as rime and graupel. Rime, consisting of frozen cloud droplets, is observed on all types of snow crystals including needles, columns, plates, and dendrites. The droplets, which vary in size from 10 to 100 μm, frequently accumulate along one face of a single snow crystal, but are found more randomly distributed on aggregations consisting of two or more snow crystals (snowflakes). The early stages of riming are characterized by the presence of frozen cloud droplets that appear as a layer of flattened hemispheres on the surface of the snow crystal. As this process continues, the cloud droplets appear more sinuous and elongate as they contact and freeze to the rimed crystals. The advanced stages of this process result in graupel, a particle 1 to 3 mm across, composed of hundreds of frozen cloud droplets interspersed with considerable air spaces; the original snow crystal is no longer discernible. This study increases our knowledge about the process and characteristics of riming and suggests that the initial appearance of the flattened hemispheres may result from impact of the leading face of the snow crystal with cloud droplets. The elongated and sinuous configurations of frozen cloud droplets that are encountered on the more advanced stages suggest that aerodynamic forces propel cloud droplets to the trailing face of the descending crystal where they make contact and freeze.

Protected electrode structures and methods

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mikhaylik, Yuriy V.; Laramie, Michael G.; Kopera, John Joseph Christopher

2017-08-08

An electrode structure and its method of manufacture are disclosed. The disclosed electrode structures may be manufactured by depositing a first release layer on a first carrier substrate. A first protective layer may be deposited on a surface of the first release layer and a first electroactive material layer may then be deposited on the first protective layer. The first release layer may have a low mean peak to valley surface roughness, which may enable the formation of a thin protective layer with a low mean peak to valley surface roughness.

Flow Pathways of Snow and Ground Ice Melt Water During Initial Seasonal Thawing of the Active Layer on Continuous Permafrost

NASA Astrophysics Data System (ADS)

Sjoberg, Y.; Johansson, E.; Rydberg, J.

2017-12-01

In most arctic environments, the snowmelt is the main hydrologic event of the year as a large fraction of annual precipitation rapidly moves through the catchment. Flow can occur on top of the frozen ground surface or through the developing active layer, and flow pathways are critical determinants for biogeochemical transport. We study the linkages between micro topography, active layer thaw, and water partitioning on a hillslope in Greenland during late snowmelt season to explore how seasonal subsurface flow pathways develop. During snowmelt, a parallel surface drainage pattern appears across the slope, consisting of small streams, and water also collects in puddles across the slope. Thaw rates in the active layer were significantly higher (T-test p<0.01) on wet parts of the slope (0.8 cm/day), compared to drier parts of the slope (0.6 cm/day). Analyses of stable water isotopic composition show that snow had the lightest isotopic signatures, but with a large spread of values, while seasonally frozen ground and standing surface water (puddles) were heavier. The stream water became heavier over the two-week sampling period, suggesting an increasing fraction of melted soil water input over time. In contrast, standing surface water (puddles) isotopic composition did not change over time. In boreal catchments, seasonal frost has previously been found to not significantly influence flow pathways during most snowmelt events, and pre-event groundwater make out most of the stream water during snowmelt. Our results from a continuous permafrost environment show that both surface (overland) and subsurface flow pathways in the active layer are active, and that a large fraction of the water moving on the hillslope comes from melted ground ice rather than snow in the late snowmelt season. This suggests a possibility that flow pathways during snowmelt could shift to deeper subsurface flow following degradation of continuous permafrost.

Effect of different cryo-protectants on the viability of frozen/thawed semen from boars of the Piau breed.

PubMed

Pinho, R O; Lima, D M A; Shiomi, H H; Siqueira, J B; Silva, H T; Lopes, P S; Guimarães, S E F; Guimarães, J D

2014-05-01

The objective of this study was to evaluate the effect of different cryo-protectants (glycerol, dimethylacetamide and dimethylformamide alone or combined and added to lactose-egg yolk extender) on the viability of frozen/thawed semen from the Piau breed as assessed by in vitro testing. Frozen semen samples (n=20) were used from five male swine. Five different freezing extenders, including 2% glycerol (Group 1 - G), 2% glycerol and 3% dimethylacetamide (Group 2 - GA), 2% glycerol and 3% dimethylformamide (Group 3 - GF), 5% dimethylacetamide (Group 4 - A) and 5% dimethylformamide (group 5 - F), were evaluated. To assess post-thawing sperm quality, sperm motility and morphology were evaluated. Sperm viability was determined using the hypoosmotic swelling test, supravital staining, and a fluorescent assay (carboxyfluorescein diacetate and propidium iodide). The mean total sperm motility of semen immediately after thawing was 46.2±1.3, 57.7±1.5, 53.2±2.1, 51.7±1.2, and 46.5±1.6% for groups 1-5, respectively. Groups 2 (GA) and 3 (GF) had greater motility values (P<0.05). Fluorescent assay values of 22.3±2.3%, 35.2±3.7%, 30.8±3.4%, 36.6±3.7%, and 26.5±3.8% were obtained for Groups 1-5, respectively, showing that Group 4 (A) sperm had greater viability than those from Group 1 (G), although there was no differences between the other treatments (P>0.05). The other complementary tests (hypoosmotic swelling test and supra-vital staining) demonstrated that sperm in Groups 2 (GA), 3 (GF) and 4 (A) had the greatest viability and there were no significant differences among these three groups (P>0.05). The most effective cryo-protectant combinations likely minimized and controlled the deleterious processes that occur in the sperm cell during freezing/thawing, thus improving post-thawing sperm viability. In conclusion, the combination of amides (3%) and glycerol (2%) or dimethylacetamide (5%) alone were more efficient at cryo-protection than glycerol alone for semen freezing in the Piau swine breed. Copyright © 2014 Elsevier B.V. All rights reserved.

Radioprotection provides functional mechanics but delays healing of irradiated tendon allografts after ACL reconstruction in sheep.

PubMed

Seto, Aaron U; Culp, Brian M; Gatt, Charles J; Dunn, Michael

2013-12-01

Successful protection of tissue properties against ionizing radiation effects could allow its use for terminal sterilization of musculoskeletal allografts. In this study we functionally evaluate Achilles tendon allografts processed with a previously developed radioprotective treatment based on (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) crosslinking and free radical scavenging using ascorbate and riboflavin, for ovine anterior cruciate ligament reconstruction. Arthroscopic anterior cruciate ligament (ACL) reconstruction was performed using double looped allografts, while comparing radioprotected irradiated and fresh frozen allografts after 12 and 24 weeks post-implantation, and to control irradiated grafts after 12 weeks. Radioprotection was successful at preserving early subfailure mechanical properties comparable to fresh frozen allografts. Twelve week graft stiffness and anterior-tibial (A-T) translation for radioprotected and fresh frozen allografts were comparable at 30 % of native stiffness, and 4.6 and 5 times native A-T translation, respectively. Fresh frozen allograft possessed the greatest 24 week peak load at 840 N and stiffness at 177 N/mm. Histological evidence suggested a delay in tendon to bone healing for radioprotected allografts, which was reflected in mechanical properties. There was no evidence that radioprotective treatment inhibited intra-articular graft healing. This specific radioprotective method cannot be recommended for ACL reconstruction allografts, and data suggest that future efforts to improve allograft sterilization procedures should focus on modifying or eliminating the pre-crosslinking procedure.

Two-Dimensional Phosphorene-Derived Protective Layers on a Lithium Metal Anode for Lithium-Oxygen Batteries.

PubMed

Kim, Youngjin; Koo, Dongho; Ha, Seongmin; Jung, Sung Chul; Yim, Taeeun; Kim, Hanseul; Oh, Seung Kyo; Kim, Dong-Min; Choi, Aram; Kang, Yongku; Ryu, Kyoung Han; Jang, Minchul; Han, Young-Kyu; Oh, Seung M; Lee, Kyu Tae

2018-05-04

Lithium-oxygen (Li-O 2 ) batteries are desirable for electric vehicles because of their high energy density. Li dendrite growth and severe electrolyte decomposition on Li metal are, however, challenging issues for the practical application of these batteries. In this connection, an electrochemically active two-dimensional phosphorene-derived lithium phosphide is introduced as a Li metal protective layer, where the nanosized protective layer on Li metal suppresses electrolyte decomposition and Li dendrite growth. This suppression is attributed to thermodynamic properties of the electrochemically active lithium phosphide protective layer. The electrolyte decomposition is suppressed on the protective layer because the redox potential of lithium phosphide layer is higher than that of electrolyte decomposition. Li plating is thermodynamically unfavorable on lithium phosphide layers, which hinders Li dendrite growth during cycling. As a result, the nanosized lithium phosphide protective layer improves the cycle performance of Li symmetric cells and Li-O 2 batteries with various electrolytes including lithium bis(trifluoromethanesulfonyl)imide in N,N-dimethylacetamide. A variety of ex situ analyses and theoretical calculations support these behaviors of the phosphorene-derived lithium phosphide protective layer.

Anomalous plasma diffusion and the magnetopause boundary layer

NASA Technical Reports Server (NTRS)

Treumann, Rudolf A.; Labelle, James; Haerendel, Gerhard; Pottelette, Raymond

1992-01-01

An overview of the current state of anomalous diffusion research at the magnetopause and its role in the formation of the magnetopause boundary layer is presented. Plasma wave measurements in the boundary layer indicate that most of the relevant unstable wave modes contribute negligibly to the diffusion process at the magnetopause under magnetically undisturbed northward IMF conditions. The most promising instability is the lower hybrid drift instability, which may yield diffusion coefficients of the right order if the highest measured wave intensities are assumed. It is concluded that global stationary diffusion due to wave-particle interactions does not take place at the magnetopause. Microscopic wave-particle interaction and anomalous diffusion may contribute to locally break the MD frozen-in conditions and help in transporting large amounts of magnetosheath plasma across the magnetospheric boundary.

Interrelated structures of the transport shock and collisional relaxation layer in a multitemperature, multilevel ionized gas

NASA Technical Reports Server (NTRS)

Vinolo, A. R.; Clarke, J. H.

1973-01-01

The gas dynamic structures of the transport shock and the downstream collisional relaxation layer are evaluated for partially ionized monatomic gases. Elastic and inelastic collisional nonequilibrium effects are taken into consideration. In the microscopic model of the atom, three electronic levels are accounted for. By using an asymptotic technique, the shock morphology is found on a continuum flow basis. This procedure gives two distinct layers in which the nonequilibrium effects to be considered are different. A transport shock appears as the inner solution to an outer collisional relaxation layer. The results show four main interesting points: (1) on structuring the transport shock, ionization and excitation rates must be included in the formulation, since the flow is not frozen with respect to the population of the different electronic levels; (2) an electron temperature precursor appears at the beginning of the transport shock; (3) the collisional layer is rationally reduced to quadrature for special initial conditions, which (4) are obtained from new Rankine-Hugoniot relations for the inner shock.

Optimizing Catalysts for Solar Fuel Production: Spectroscopic Characterization of the Key Reaction Intermediates

DTIC Science & Technology

2013-04-01

which freezes ions into well defined structures and coats them with an inert layer of weakly bound adducts. These cold aggregates were then...evaporation of the cryogenic solvent. Instrument development. Cryogenic ion processing. Cold ion spectroscopy. Trapped reaction intermediates U U U...spectrometer. The key advance incorporated into this instrument is the introduction of a cryogenic (10K) ion processing stage, where ions can be frozen

Subchronic treatment with acai frozen pulp prevents the brain oxidative damage in rats with acute liver failure.

PubMed

de Souza Machado, Fernanda; Kuo, Jonnsin; Wohlenberg, Mariane Farias; da Rocha Frusciante, Marina; Freitas, Márcia; Oliveira, Alice S; Andrade, Rodrigo B; Wannmacher, Clovis M D; Dani, Caroline; Funchal, Claudia

2016-12-01

Acai has been used by the population due to its high nutritional value and its benefits to health, such as its antioxidant properties. The aim of this study was to evaluate the protective effect of acai frozen pulp on oxidative stress parameters in cerebral cortex, hippocampus and cerebellum of Wistar rats treated with carbon tetrachloride (CCl 4 ). Thirty male Wistar rats (90-day-old) were orally treated with water or acai frozen pulp for 14 days (7 μL/g). On the 15th day, half of the animals received treatment with mineral oil and the other half with CCl 4 (3.0 mL/kg). The cerebral cortex, hippocampus and cerebellum were dissected and used for analysis of creatine kinase activity (CK), thiobarbituric acid reactive substances (TBARS), carbonyl, sulfhydryl, and the activity of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). Statistical analysis was performed by ANOVA followed by Tukey's post-test. CCl 4 was able to inhibit CK activity in all tissues tested and to provoke lipid damage in cerebral cortex and cerebellum, and protein damage in the three tissues tested. CCl 4 enhanced CAT activity in the cerebral cortex, and inhibited CAT activity in the hippocampus and cerebellum and reduced SOD activity in all tissues studied. Acai frozen pulp prevented the inhibition of CK, TBARS, carbonyl and CAT activity in all brain structures and only in hippocampus for SOD activity. Therefore, acai frozen pulp has antioxidant properties and maybe could be useful in the treatment of some diseases that affect the central nervous system that are associated with oxidative damage.

Spawn viability in edible mushrooms after freezing in liquid nitrogen without a cryoprotectant.

PubMed

Mata, Gerardo; Pérez-Merlo, Rosalía

2003-08-01

Five strains of edible mushrooms (Lentinula boryana, Lentinula edodes, Pleurotus djamor, Pleurotus pulmonarius, and Volvariella volvacea) were studied. Spawn were prepared from sorghum seeds and then incubated for 14 days under optimum conditions for each species. Once covered by mycelia, the sorghum seeds were placed in polycarbonate vials for freezing in liquid nitrogen. The effect of adding a cryoprotective solution before freezing (either 10% glycerol v/v or 5% dimethylsulfoxide v/v) was evaluated as a function of mycelial growth and percent viability. Three main treatments were undertaken: (1) freezing with a glycerol or dimethylsulfoxide cryoprotectant, (2) freezing with water and (3) freezing without cryoprotectant or water. Samples were maintained frozen for a week, after which time they were thawed (10 min at 30 degrees C) and the seeds placed in Petri dishes with a culture medium. A recovery rate of 96.8% was obtained for the total number of samples summed over all strains and treatments. In contrast, 99.2% of the samples frozen without cryoprotectant were recovered. The recovery of frozen mycelia was delayed with respect to a control group, which was not frozen. However, no difference was observed in percent recovery and mycelial diameter when a new series of spawn was prepared from mycelia that had been previously frozen. Results obtained from this experiment demonstrate that an adequate recovery of mycelia can be obtained without using a cryoprotectant. This capacity might enable large quantities of commercial mushroom strains to be handled at reduced production costs. It is suggested that the mycelia survived freezing without cryoprotectants because they were embedded and protected within the sorghum seeds used to elaborate the spawn.

Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing-thawing.

PubMed

Shen, Tao; Jiang, Zhong-Liang; Liu, Hong; Li, Qing-Wang

2015-08-01

Salvia miltiorrhiza polysaccharides (SMPs) were extracted from S. miltiorrhiza in this study. The aim of the present study was to evaluate the effect of SMP on the motility of boar sperm, including the antioxidant effect of SMP on boar sperm and the effect of SMP on the in vivo fertilizing ability of frozen-thawed boar sperm. Fifty ejaculates from 5 Swagger boars were collected and diluted with an extender, which contained 3% glycerol (v/v) with five concentrations of SMP (0.2, 0.4, 0.6, 0.8, and 1.0mg/mL). The semen was frozen in 0.25mL straws at 1.0×10(9) cells/mL. Sixty gilts were inseminated using fresh semen, frozen semen with 0.4mg/mL of SMP and frozen semen without SMP. The results indicate that the addition of SMP to the extender results in a higher percentage of motile sperm post-thaw (P<0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxalacetic transaminease and catalase were all determined to be significantly higher than the control group after adding SMP to the extender (P<0.05). The artificial insemination (AI) results demonstrated that the litter size was significantly higher in the 0.4mg/mL of SMP group than in the control group (P<0.05). In conclusion, during the process of freezing, SMP can protect boar sperm from peroxidative damage and increase sperm motility and litter size during the process of freezing-thawing. The optimal concentration of SMP for the frozen extenders in this study was determined to be 0.4mg/mL. Copyright © 2015. Published by Elsevier B.V.

Presence of rapidly degrading permafrost plateaus in south-central Alaska

USGS Publications Warehouse

Jones, Benjamin M.; Baughman, Carson; Romanovsky, Vladimir E.; Parsekian, Andrew D.; Babcock, Esther; Stephani, Eva; Jones, Miriam C.; Grosse, Guido; Berg, Edward E

2016-01-01

Permafrost presence is determined by a complex interaction of climatic, topographic, and ecological conditions operating over long time scales. In particular, vegetation and organic layer characteristics may act to protect permafrost in regions with a mean annual air temperature (MAAT) above 0 °C. In this study, we document the presence of residual permafrost plateaus in the western Kenai Peninsula lowlands of south-central Alaska, a region with a MAAT of 1.5 ± 1 °C (1981–2010). Continuous ground temperature measurements between 16 September 2012 and 15 September 2015, using calibrated thermistor strings, documented the presence of warm permafrost (−0.04 to −0.08 °C). Field measurements (probing) on several plateau features during the fall of 2015 showed that the depth to the permafrost table averaged 1.48 m but at some locations was as shallow as 0.53 m. Late winter surveys (augering, coring, and GPR) in 2016 showed that the average seasonally frozen ground thickness was 0.45 m, overlying a talik above the permafrost table. Measured permafrost thickness ranged from 0.33 to  >  6.90 m. Manual interpretation of historic aerial photography acquired in 1950 indicates that residual permafrost plateaus covered 920 ha as mapped across portions of four wetland complexes encompassing 4810 ha. However, between 1950 and ca. 2010, permafrost plateau extent decreased by 60.0 %, with lateral feature degradation accounting for 85.0 % of the reduction in area. Permafrost loss on the Kenai Peninsula is likely associated with a warming climate, wildfires that remove the protective forest and organic layer cover, groundwater flow at depth, and lateral heat transfer from wetland surface waters in the summer. Better understanding the resilience and vulnerability of ecosystem-protected permafrost is critical for mapping and predicting future permafrost extent and degradation across all permafrost regions that are currently warming. Further work should focus on reconstructing permafrost history in south-central Alaska as well as additional contemporary observations of these ecosystem-protected permafrost sites south of the regions with relatively stable permafrost.

Lens and dendrite formation during colloidal solidification

NASA Astrophysics Data System (ADS)

Worster, Grae; You, Jiaxue

2017-11-01

Colloidal particles in suspension are forced into a variety of morphologies when the suspending fluid medium is frozen: soil is compacted between ice lenses during frost heave; ice templating is a recent and growing technology to produce bio-inspired, micro-porous materials; cells and tissue can be damaged during cryosurgery; and metal-matrix composites with tailored microstructure can be fabricated by controlled casting. Various instabilities that affect the microscopic morphology are controlled by fluid flow through the compacted layer of particles that accumulates ahead of the solidification front. By analysing the flow in connection with equilibrium phase relationships, we develop a theoretical framework that identifies two different mechanisms for ice-lens formation, with and without a frozen fringe, identifies the external parameters that differentiates between them and the possibility of dendritic formations, and unifies a range of apparently disparate conclusions drawn from previous experimental studies. China Scholarship Council and the British Council.

Adsorption-induced conformational changes of antifreeze glycoproteins at the ice/water interface.

PubMed

Uda, Yukihiro; Zepeda, Salvador; Kaneko, Fumitoshi; Matsuura, Yoshiki; Furukawa, Yoshinori

2007-12-27

The conformation of antifreeze glycoprotein (AFGP) molecules adsorbed at the ice/water interface was studied by attenuated total reflection (ATR)-FTIR spectroscopy. Measurements were carried out for AFGP/D2O solution films formed on the surface of an ATR prism as a function of temperature. Using the FTIR spectrum from the O-D stretching band of D2O molecules, we monitored the supercooled and frozen states of the film and measured the thickness of the quasi-liquid layer (QLL) at the ice/prism interfaces. The AFGP structure was determined for the liquid, supercooled, and frozen states of the solution film using the amide I band spectra. No noticeable differences in conformation were observed in the solution conformation from room temperature down to the 15 K supercooling studied, whereas the alpha-helical content of AFGP suddenly increased when the supercooled solution film froze at -15 degrees C. This change in conformation can increase the overall interaction between the AFGP molecules and ice surface and allow a stronger adsorption. In contrast, the alpha-helical content of AFGP in the frozen film gradually decreased with increasing temperature and finally returned to its solution-state level at the melting point of D2O ice. This gradual decrease in the alpha-helix content directly correlates with the measured increase in QLL thickness. Finally, we conclude that the differences in the alpha-helix signals between the frozen and supercooled states indicate the conformational change of AFGP molecules upon adsorption at the ice/water interface, emphasizing the importance of the structure-function relationship, even for this highly flexible antifreeze.

WISDOM GPR performance assessment in a cold artificial environment

NASA Astrophysics Data System (ADS)

Dechambre, M.; Ciarletti, V.; Biancheri-Astier, M.; Saintenoy, A.; Costard, F.; Hassen-Khodja, R.

2012-04-01

The WISDOM (Water Ice Subsurface Deposit Observation on Mars) GPR is one of the instruments that have been selected as part of the Pasteur payload of ESA's 2018 ExoMars Rover mission. WISDOM has been designed to obtain information about the nature of the subsurface along the rover path with the objective to explore the first ~ 3 m of the soil with a vertical resolution of a few centimetres. The sub-surface properties that can be addressed with WISDOM are variations in composition, texture, stratification (e.g., number, thickness and orientation of layers), the presence of unconformities and other structural characteristics (such as fractures and the deformation of strata). It is then essential to quantify the performances of WISDOM in controlled conditions, and several full polarimetric measurements have been carried out with the prototype in a cold artificial environment. The main objectives are the detection of different interface between homogeneous materials with WISDOM. The characterization of the material (porosity, % of water, dielectric properties, thickness and depth, temperature ...) is well-controlled. The cold room facility of IDES at Orsay (France) has been used, the ambient temperature ranged from -7° C to -10° C. A tank laying on the metallic floor (height: 0.5m, width: 0.80 m, length: 1.20m) in macrolon can contain liquid or frozen water or layers (dielectric contrasts) of home-maid permafrost (frozen saturated sand) with and without embedded objects or fractures. The temperature inside the medium (ice or permafrost) is controlled, the radar antennas are put on a sheet of polystyrene over the tank. Frequent measurements were performed (every 2cm) along a track from one side to the other side of the tank. The experimental conditions were: (1)dry cold sand (Fontainebleau sand) : porosity 35% density 2,67 (2) saturated wet sand : 35% of water (3) permafrost (frozen saturated sand) : 35% of ice content 1 layer: 3 consecutive experiments : 10cm dry sand ( 1) 10cm saturated sand (2) 10cm permafrost(3) 2 layers :previous 10cm permafrost in the bottom +3 consecutive experiments : 10cm dry sand ( 1) 10cm saturated sand (2) 10cm permafrost(3) . Basalt rocks and air fractures are or are not embedded in the layers Values of the permittivity of dry sand and permafrost were retrieve by two different ways. 1.Retrieval of the sand and permafrost permittivity from delay measurements knowing the layer thickness d ɛr = ct/2d = n2 2. Retrieval of the sand and permafrost permittivity from amplitude measurements knowing a calibration reference (reflection over a metallic plate), R is the Fresnel coefficient between the air and the medium. Aint/ = R = n- 1-,ɛ = 1+-R-= n2 Aair n +1 r 1- R Results : Internal layering is observed. The transition between dry sand and permafrost can be detected. The permittivity can be retrieved from delay or amplitude measurements as well from delay measurements : dry sand ɛr = 2.71 permafrost ɛr = 3.72 from amplitude measurements : dry sand ɛr = 2.73 permafrost ɛr = 3.35 Embedded objects are detected, Fracture and its orientation is detected.

Multilayer graphene as an effective corrosion protection coating for copper

NASA Astrophysics Data System (ADS)

Ravishankar, Vasumathy; Ramaprabhu, S.; Jaiswal, Manu

2018-04-01

Graphene grown by chemical vapor deposition (CVD) has been studied as a protective layer against corrosion of copper. The layer number dependence on the protective nature of graphene has been investigated using techniques such as Tafel analysis and Electroimpedance Spectroscopy. Multiple layers of graphene were achieved by wet transfer above CVD grown graphene. Though this might cause grain boundaries, the sites where corrosion is initiated, to be staggered, wet transfer inherently carries the disadvantage of tearing of graphene, as confirmed by Raman spectroscopy measurements. However, Electroimpedance Spectroscopy (EIS) reflects that graphene protected copper has a layer dependent resistance to corrosion. Decrease in corrosion current (Icorr) for graphene protected copper is presented. There is only small dependence of corrosion current on the layer number, Tafel plots clearly indicate passivation in the presence of graphene, whether it be single layer or multiple layers. Notwithstanding the crystallite size, defect free layers of graphene with staggered grain boundaries combined with passivation could offer good corrosion protection for metals.

Fill and spill drives runoff connectivity over frozen ground

NASA Astrophysics Data System (ADS)

Coles, A. E.; McDonnell, J. J.

2018-03-01

Snowmelt-runoff processes on frozen ground are poorly understood at the hillslope scale. This is especially true for hillslopes on the northern Great Plains of North America where long periods of snow-covered frozen ground with very shallow slopes mask any spatial patterns and process controls on connectivity and hillslope runoff generation. This study examines a 4.66 ha (46,600 m2) hillslope on the northern Great Plains during the 2014 spring snowmelt season to explore hillslope runoff processes. Specifically, we explore the spatial patterns of runoff production source areas and examine how surface topography and patterns of snow cover, snow water equivalent, soil water content, and thawed layer depth - which we measured on a 10 m grid across our 46,600 m2 hillslope - affect melt water partitioning and runoff connectivity. A key question was whether or not the controls on connectivity are consistent with the fill and spill mechanism found in rain-dominated and unfrozen soil domains. The contrast between the slow infiltration rates into frozen soil and the relatively fast rates of snowmelt delivery to the soil surface resulted in water accumulation in small depressions under the snowpack. Consequently, infiltration was minimal over the 12 day melt period. Instead, nested filling of micro- and meso-depressions was followed by macro-scale, whole-slope spilling. This spilling occurred when large patches of ponded water exceeded the storage capacity behind downslope micro barriers in the surface topography, and flows from them coalesced to drive a rapid increase in runoff at the hillslope outlet. These observations of ponded water and flowpaths followed mapable fill and spill locations based on 2 m resolution digital topographic analysis. Interestingly, while surface topography is relatively unimportant under unfrozen conditions at our site because of low relief and high infiltrability, surface topography shows episodically critical importance for connectivity and runoff generation when the ground is frozen.

Methane emission through ebullition from an estuarine mudflat: 2. Field observations and modeling of occurrence probability

NASA Astrophysics Data System (ADS)

Chen, Xi; Schäfer, Karina V. R.; Slater, Lee

2017-08-01

Ebullition can transport methane (CH4) at a much faster rate than other pathways, albeit over limited time and area, in wetland soils and sediments. However, field observations present large uncertainties in ebullition occurrences and statistic models are needed to describe the function relationship between probability of ebullition occurrence and water level changes. A flow-through chamber was designed and installed in a mudflat of an estuarine temperate marsh. Episodic increases in CH4 concentration signaling ebullition events were observed during ebbing tides (15 events over 456 ebbing tides) and occasionally during flooding tides (4 events over 455 flooding tides). Ebullition occurrence functions were defined using logistic regression as the relative initial and end water levels, as well as tidal amplitudes were found to be the key functional variables related to ebullition events. Ebullition of methane was restricted by a surface frozen layer during winter; melting of this layer during spring thaw caused increases in CH4 concentration, with ebullition fluxes similar to those associated with large fluctuations in water level around spring tides. Our findings suggest that initial and end relative water levels, in addition to tidal amplitude, partly regulate ebullition events in tidal wetlands, modulated by the lunar cycle, storage of gas bubbles at different depths and seasonal changes in the surface frozen layer. Maximum tidal strength over a few days, rather than hourly water level, may be more closely associated with the possibility of ebullition occurrence as it represents a trade-off time scale in between hourly and lunar periods.

75 FR 4350 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Preliminary Results of New...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-27

... From the Socialist Republic of Vietnam: Preliminary Results of New Shipper Review AGENCY: Import...'') of August 1, 2008, through January 31, 2009. If these preliminary results are adopted in our final results of review, we will instruct U.S. Customs and Border Protection (``CBP'') to assess antidumping...

29 CFR 1926.350 - Gas welding and cutting.

Code of Federal Regulations, 2010 CFR

2010-07-01

... or valve protection caps to pry cylinders loose when frozen. Warm, not boiling, water shall be used... fuel gas cylinder is opened, there is found to be a leak around the valve stem, the valve shall be closed and the gland nut tightened. If this action does not stop the leak, the use of the cylinder shall...

Ozone Layer Protection

MedlinePlus

... Offices Labs and Research Centers Contact Us Share Ozone Layer Protection The stratospheric ozone layer is Earth’s “ ... to ozone-depleting substances, and sun safety. Stratospheric Ozone Layer Basic Ozone Layer Science Health and Environmental ...

Formation of lacustrine plains in west-central Alaska as a result of permafrost degradation and aggradation

NASA Astrophysics Data System (ADS)

Kanevskiy, M. Z.; Jorgenson, M. T.; Shur, Y.; O'Donnell, J.; Harden, J. W.; Fortier, D.

2012-12-01

Perennially frozen lacustrine sediments containing a large amount of ground ice comprise a significant part of the upper permafrost of the lowlands of west-central Alaska, including Koyukuk Flats and Innoko Flats. Study sites are located in the discontinuous permafrost zone, where permafrost was encountered mainly within uplifted peat plateaus. The upper part of studied sections is formed by frozen peat up to 3 m thick underlain by lacustrine silt, which is mostly ice-rich. Cryogenic structure of lacustrine sediments at different sites has common features: (1) prevalence of layered, braided, and reticulate cryostructures; (2) high variability in the ice content of sediments; (3) high density and low water content of soil aggregates separated by ice lenses. Volume of visible ice in silt reaches at places 40% and more. The thickness of ice lenses generally varies from 1 to 5 cm and occasionally reaches 10 cm. Remnants of peat plateaus are surrounded by unfrozen bogs and fens, formed as a result of thawing and settling of ice-rich lacustrine silt. Modern thermokarst scars initially form at places where ice-rich silt is not protected by a thick layer of organic material. Further development of thermokarst bogs includes lateral enlargement of thaw bulbs and collapsing of the margins of peat plateaus. Lacustrine silt within taliks is covered by woody peat accumulated under forests during the stage of permafrost plateau formation and then by aquatic sphagnum peat accumulated in taliks after collapse. We relate the formation of ice-rich lacustrine sediments to development of lake thermokarst, which affected ice-rich silty yedoma deposits during the transition from Pleistocene to Holocene. Terrain development in lacustrine lowlands of west-central Alaska includes five stages related to permafrost aggradation and degradation from the late Pleistocene to the present time: 1) formation of the ice-rich syngenetic permafrost (yedoma) during the late Pleistocene; 2) yedoma degradation in the yearly Holocene and formation of thaw lakes; 3) complete yedoma degradation under thaw lakes and refreezing of thawed sediments at elevated areas; 4) peat accumulation and freezing of sediments in thaw lake basins; and 5) new cycle of thermokarst and formation of taliks under thaw lakes, bogs and fens. Stages of terrain development of lacustrine lowlands since the Late Pleistocene

The corrosion protection of AA2024-T3 aluminium alloy by leaching of lithium-containing salts from organic coatings.

PubMed

Visser, Peter; Liu, Yanwen; Zhou, Xiaorong; Hashimoto, Teruo; Thompson, George E; Lyon, Stuart B; van der Ven, Leendert G J; Mol, Arjan J M C; Terryn, Herman A

2015-01-01

Lithium carbonate and lithium oxalate were incorporated as leachable corrosion inhibitors in model organic coatings for the protection of AA2024-T3. The coated samples were artificially damaged with a scribe. It was found that the lithium-salts are able to leach from the organic coating and form a protective layer in the scribe on AA2024-T3 under neutral salt spray conditions. The present paper shows the first observation and analysis of these corrosion protective layers, generated from lithium-salt loaded organic coatings. The scribed areas were examined by scanning and transmission electron microscopy before and after neutral salt spray exposure (ASTM-B117). The protective layers typically consist of three different layered regions, including a relatively dense layer near the alloy substrate, a porous middle layer and a flake-shaped outer layer, with lithium uniformly distributed throughout all three layers. Scanning electron microscopy and white light interferometry surface roughness measurements demonstrate that the formation of the layer occurs rapidly and, therefore provides an effective inhibition mechanism. Based on the observation of this work, a mechanism is proposed for the formation of these protective layers.

Rapid runoff via shallow throughflow and deeper preferential flow in a boreal catchment underlain by frozen silt (Alaska, USA)

USGS Publications Warehouse

Koch, Joshua C.; Ewing, Stephanie A.; Striegl, Robert G.; McKnight, Diane M.

2013-01-01

In high-latitude catchments where permafrost is present, runoff dynamics are complicated by seasonal active-layer thaw, which may cause a change in the dominant flowpaths as water increasingly contacts mineral soils of low hydraulic conductivity. A 2-year study, conducted in an upland catchment in Alaska (USA) underlain by frozen, well-sorted eolian silt, examined changes in infiltration and runoff with thaw. It was hypothesized that rapid runoff would be maintained by flow through shallow soils during the early summer and deeper preferential flow later in the summer. Seasonal changes in soil moisture, infiltration, and runoff magnitude, location, and chemistry suggest that transport is rapid, even when soils are thawed to their maximum extent. Between June and September, a shift occurred in the location of runoff, consistent with subsurface preferential flow in steep and wet areas. Uranium isotopes suggest that late summer runoff erodes permafrost, indicating that substantial rapid flow may occur along the frozen boundary. Together, throughflow and deep preferential flow may limit upland boreal catchment water and solute storage, and subsequently biogeochemical cycling on seasonal to annual timescales. Deep preferential flow may be important for stream incision, network drainage development, and the release of ancient carbon to ecosystems

A Novel Adjustable Concept for Permeable Gas/Vapor Protective Clothing: Balancing Protection and Thermal Strain.

PubMed

Bogerd, Cornelis Peter; Langenberg, Johannes Pieter; DenHartog, Emiel A

2018-02-13

Armed forces typically have personal protective clothing (PPC) in place to offer protection against chemical, biological, radiological and nuclear (CBRN) agents. The regular soldier is equipped with permeable CBRN-PPC. However, depending on the operational task, these PPCs pose too much thermal strain to the wearer, which results in a higher risk of uncompensable heat stress. This study investigates the possibilities of adjustable CBRN-PPC, consisting of different layers that can be worn separately or in combination with each other. This novel concept aims to achieve optimization between protection and thermal strain during operations. Two CBRN-PPC (protective) layers were obtained from two separate manufacturers: (i) a next-to-skin (NTS) and (ii) a low-burden battle dress uniform (protective BDU). In addition to these layers, a standard (non-CBRN protective) BDU (sBDU) was also made available. The effect of combining clothing layers on the levels of protection were investigated with a Man-In-Simulant Test. Finally, a mechanistic numerical model was employed to give insight into the thermal burden of the evaluated CBRN-PPC concepts. Combining layers results in substantially higher protection that is more than the sum of the individual layers. Reducing the airflow on the protective layer closest to the skin seems to play an important role in this, since combining the NTS with the sBDU also resulted in substantially higher protection. As expected, the thermal strain posed by the different clothing layer combinations decreases as the level of protection decreases. This study has shown that the concept of adjustable protection and thermal strain through multiple layers of CBRN-PPC works. Adjustable CBRN-PPC allows for optimization of the CBRN-PPC in relation to the threat level, thermal environment, and tasks at hand in an operational setting. © The Author(s) 2017. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

Change in Spatial Distribution of Permafrost in the Source Area of the Yellow River: A Numerical Prediction

NASA Astrophysics Data System (ADS)

Ma, S.; Sheng, Y.; Wu, J.; Hu, X.; Li, J.

2017-12-01

Permafrost plays an important role in the climate system through its influence on energy exchanges, hydrological processes, natural hazards and carbon budgets. As a response to the global warming, permafrost is degrading with various manifestations, such as increase in permafrost temperature, thickening of active layer, permafrost disappearance. The Source Area of the Yellow River is located in the mosaic transition zones of seasonally frozen ground, and discontinuous and continuous permafrost on the northeastern Qinghai-Tibet Plateau. Based on the prediction results of the climate model in the IPCC Fifth Assessment Report, this article attempts to forecast the change of the typical permafrost types in the SAYR by using the numerical simulation method. And we calculate the spatial distribution of permafrost in the past and predict the change trend of permafrost in the future. The results show that only a small part of the permafrost in this region has degraded in1972 2012 and the degraded area is about 279 km2. The seasonal frozen soil is mainly distributed in the valley of Re Qu, Xiaoyemaling and Tangchama in the south of the two lake basins. There is little area difference on the permafrost degrading into the seasonal frozen soil under the scenarios of RCP2.6, RCP6.0, RCP8.5 in 2050. The degrading area of permafrost is 2224 km2, 2347 km2, 2559 km2. They account for 7.5%, 7.9%, 8.6% of the Source Area, respectively. And the seasonal frozen soil is sporadically distributed in Lena Qu, Duo Qu, Baima Qu. They widely spread on Yeniugou, Yeniutan and four Madio lakes being located in the Yellow River valley of the eastern part of Ngoring Lake. In 2100, the area of permafrost degradation is 5636 km2, 9769 km2, 15548 km2. They accounts for 19%, 32.9% and 52.3% of the source area, respectively. The permafrost mainly degenerate in the area of Xingsuhai, Gamaletan, Duogerong. Permafrost influences hydrology by providing an impermeable barrier to the movement of liquid water. The decrease in ground ice content, as caused by permafrost degradation, facilitates the percolation of more water to deeper soil layers, thus resulting in the reallocation of runoff. These results provide useful references for evaluating the level of permafrost degradation in response to climate warming on the SAYR.

Electrode structure and method for making the same

DOEpatents

Affinito, John D.; Lowe, Gregory K.

2015-05-26

Electrode structures, and more specifically, electrode structures for use in electrochemical cells, are provided. The electrode structures described herein may include one or more protective layers. In one set of embodiments, a protective layer may be formed by exposing a lithium metal surface to a plasma comprising ions of a gas to form a ceramic layer on top of the lithium metal. The ceramic layer may be highly conductive to lithium ions and may protect the underlying lithium metal surface from reaction with components in the electrolyte. In some cases, the ions may be nitrogen ions and a lithium nitride layer may be formed on the lithium metal surface. In other embodiments, the protective layer may be formed by converting lithium to lithium nitride at high pressures. Other methods for forming protective layers are also provided.

ARC-1989-AC89-0114-324

NASA Image and Video Library

1989-02-28

Arctic Ozone Expedition Stavanger Norway These clouds in the polar regions north of Stravanger, Norway are representative of what are called 'Type 1' and Type 2' polar stratospheric clouds. Type 1 are seen in the lower portion and consist of cloud particles comprised of nitrogen trihydrates. Seen edge-on as in this view, they appear as a thin dark orange or brown layer. Multiple layering can be seen. The Type 2 clouds are above and consist mostly of water molecules frozen as ice. The size of the particles in Type 2 clouds, the water-ice clouds, are much larger than the particle size of the Type 1 clouds - which are on the order of the wavelength of light.

Hazards protection for space suits and spacecraft

NASA Technical Reports Server (NTRS)

Kosmo, Joseph J. (Inventor); Dawn, Frederic S. (Inventor)

1990-01-01

A flexible multi-layered covering article for protection against the hazards of exposure to the environment of outer space is disclosed. The covering includes an outer layer section comprising an outermost lamina of woven expanded tetrafluoroethylene yarns (Gore Tex) for protecting against abrasion and tearing, an underlying weave of meta-aramid yarns (Nomex) and para-aramid yarns (Kevlar) for particle impart protection, and electrostatic charge dissipation and control system incorporated therein, and a chemical contaminants control barrier applied as a coating. A middle section includes a succession of thermal insulating layers of polymeric thermoplastic or thermoforming material, each of which is coated with a metal deposit of high infra-red emissivity and low solar radiation absorption characteristics and separated from adjacent insulating layers by a low thermal conductance material. The covering further includes a radiation attenuating layer of a tungsten-loaded polymeric elastomer binder for protecting against bremsstrahlung radiation and an inner layer of rip-stop polyester material for abrasion protection. A chloroprene coating may be supplied the polyester-material for added micrometeroid protection. Securing means of low heat conductance material secures the multi-layers together as a laminar composite.

Cryopreservation of veliger larvae of trumpet shell, Charonia sauliae: an essential preparation to artificial propagation

NASA Astrophysics Data System (ADS)

Kang, Kyoung Ho; Zhang, Zhifeng; Bao, Zhenmin; Shao, Mingyu

2009-09-01

Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants (ethylene glycol, 1, 2-propanediol, dimethyl sulfoxide and glycerol) were employed at three concentrations (1.0, 1.5 and 2.0 molL-1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants after 1 h storage were cultured, and then survival rates were determined at 24, 72 and 120 h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL-1 showed the best protective effect in all experiments ( p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1, 7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL-1 dimethyl sulfoxide after 1 h, 1 d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23% ±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods ( p>0.05).

Lactoferrin increases sperm membrane functionality of frozen equine semen.

PubMed

Martins, H S; da Silva, G C; Cortes, S F; Paes, F O; Martins Filho, O A; Araujo, Mss; Stahlberg, R; Lagares, M A

2018-06-01

During cryopreservation, sperm was submitted to an increase in reactive oxygen species generation. This work aimed to improve the quality of frozen equine sperm after the addition of antioxidants lactoferrin (Lf) and catalase (Cat) to a freezing extender. Semen from six stallions was frozen with the extenders: F1) control, INRA 82 freezing extender, F2) F1 + 500 μg/ml Lf and F3) F1 + 200 IU/ml Cat. After thawing, sperm motility parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome-reacted sperm were evaluated with a computer-assisted sperm analysis, a hypoosmotic swelling test and epifluorescent microscopy, respectively. Nitrite, hydroperoxide and iron concentrations of frozen semen were measured with spectrophotometry. The percentage of functional membrane sperm treated with Lf was higher (50.7% ± 11.6%) compared to that of the control (37.6% ± 15.6%), while the iron (61.4 ± 11.6 vs 73.3 ± 13.8 mg/dl) and nitrite concentrations (16.3 ± 7.1 vs 25.9 ± 4.2 μM/μg protein) were lower, respectively (p < .05). Thus, it can be suggested that Lf protect stallion spermatozoon during freezing as it has increased the percentage of sperm with functional membrane and decreased the lipid oxidant agents. © 2018 Blackwell Verlag GmbH.

Influence of dielectric protective layer on laser damage resistance of gold coated gratings

NASA Astrophysics Data System (ADS)

Wu, Kepeng; Ma, Ping; Pu, Yunti; Xia, Zhilin

2016-03-01

Aiming at the problem that the damage threshold of gold coated grating is relatively low, a dielectric film is considered on the gold coated gratings as a protective layer. The thickness range of the protective layer is determined under the prerequisite that the diffraction efficiency of the gold coated grating is reduced to an acceptable degree. In this paper, the electromagnetic field, the temperature field and the stress field distribution in the grating are calculated when the silica and hafnium oxide are used as protective layers, under the preconditions of the electromagnetic field distribution of the gratings known. The results show that the addition of the protective layer changes the distribution of the electromagnetic field, temperature field and stress field in the grating, and the protective layer with an appropriate thickness can improve the laser damage resistance of the grating.

Small molecule ice recrystallization inhibitors mitigate red blood cell lysis during freezing, transient warming and thawing

NASA Astrophysics Data System (ADS)

Briard, Jennie G.; Poisson, Jessica S.; Turner, Tracey R.; Capicciotti, Chantelle J.; Acker, Jason P.; Ben, Robert N.

2016-03-01

During cryopreservation, ice recrystallization is a major cause of cellular damage. Conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol function by a number of different mechanisms but do not mitigate or control ice recrystallization at concentrations utilized in cryopreservation procedures. In North America, cryopreservation of human red blood cells (RBCs) utilizes high concentrations of glycerol. RBC units frozen under these conditions must be subjected to a time-consuming deglycerolization process after thawing in order to remove the glycerol to <1% prior to transfusion thus limiting the use of frozen RBC units in emergency situations. We have identified several low molecular mass ice recrystallization inhibitors (IRIs) that are effective cryoprotectants for human RBCs, resulting in 70-80% intact RBCs using only 15% glycerol and slow freezing rates. These compounds are capable of reducing the average ice crystal size of extracellular ice relative to a 15% glycerol control validating the positive correlation between a reduction in ice crystal size and increased post-thaw recovery of RBCs. The most potent IRI from this study is also capable of protecting frozen RBCs against the large temperature fluctuations associated with transient warming.

Oxidative stability of cooked, frozen, reheated beef patties: effect of antioxidants.

PubMed

Colindres, Paola; Brewer, M Susan

2011-03-30

The effect of selected antioxidants (grape seed extract (GS), oleoresin rosemary (OR), water-soluble oregano extract (WO), propyl gallate (PG), butylated hydroxyanisole (BHA)), butylated hydroxytoluene (BHT)) on sensory, color and oxidative stability of cooked, frozen, reheated ground beef patties was evaluated. Beef lean and trim were ground; antioxidants and salt were added. Patties were cooked (71 °C), overwrapped in commercial polyvinyl chloride film, and stored frozen (-18 °C), then evaluated monthly for 6 months. Flavor, odor and color were determined using a descriptive panel. Instrumental color was determined by a spectrocolorimeter. Lipid oxidation was determined using thiobarbituric acid reactive substances (TBARS). After 6 months of storage, PG and GS samples had lower rancid odor scores and TBARS than controls. Control samples and those containing BHT did not differ statistically in sensory grassy or rancid odor, indicating that they were the most oxidized. TBARS correlated with grassy, rancid, cardboard and beef odors during the 6-month storage period. Based on TBARS, the order of effectiveness of the antioxidants was PG and GS > OR > BHA > WO and BHT > control. TBARS were well correlated with sensory evaluations of odor and flavor. Antioxidants also protected a* values during storage. Copyright © 2011 Society of Chemical Industry.

Small molecule ice recrystallization inhibitors mitigate red blood cell lysis during freezing, transient warming and thawing

PubMed Central

Briard, Jennie G.; Poisson, Jessica S.; Turner, Tracey R.; Capicciotti, Chantelle J.; Acker, Jason P.; Ben, Robert N.

2016-01-01

During cryopreservation, ice recrystallization is a major cause of cellular damage. Conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol function by a number of different mechanisms but do not mitigate or control ice recrystallization at concentrations utilized in cryopreservation procedures. In North America, cryopreservation of human red blood cells (RBCs) utilizes high concentrations of glycerol. RBC units frozen under these conditions must be subjected to a time-consuming deglycerolization process after thawing in order to remove the glycerol to <1% prior to transfusion thus limiting the use of frozen RBC units in emergency situations. We have identified several low molecular mass ice recrystallization inhibitors (IRIs) that are effective cryoprotectants for human RBCs, resulting in 70–80% intact RBCs using only 15% glycerol and slow freezing rates. These compounds are capable of reducing the average ice crystal size of extracellular ice relative to a 15% glycerol control validating the positive correlation between a reduction in ice crystal size and increased post-thaw recovery of RBCs. The most potent IRI from this study is also capable of protecting frozen RBCs against the large temperature fluctuations associated with transient warming. PMID:27021850

Small molecule ice recrystallization inhibitors mitigate red blood cell lysis during freezing, transient warming and thawing.

PubMed

Briard, Jennie G; Poisson, Jessica S; Turner, Tracey R; Capicciotti, Chantelle J; Acker, Jason P; Ben, Robert N

2016-03-29

During cryopreservation, ice recrystallization is a major cause of cellular damage. Conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol function by a number of different mechanisms but do not mitigate or control ice recrystallization at concentrations utilized in cryopreservation procedures. In North America, cryopreservation of human red blood cells (RBCs) utilizes high concentrations of glycerol. RBC units frozen under these conditions must be subjected to a time-consuming deglycerolization process after thawing in order to remove the glycerol to <1% prior to transfusion thus limiting the use of frozen RBC units in emergency situations. We have identified several low molecular mass ice recrystallization inhibitors (IRIs) that are effective cryoprotectants for human RBCs, resulting in 70-80% intact RBCs using only 15% glycerol and slow freezing rates. These compounds are capable of reducing the average ice crystal size of extracellular ice relative to a 15% glycerol control validating the positive correlation between a reduction in ice crystal size and increased post-thaw recovery of RBCs. The most potent IRI from this study is also capable of protecting frozen RBCs against the large temperature fluctuations associated with transient warming.

Article having an improved platinum-aluminum-hafnium protective coating

NASA Technical Reports Server (NTRS)

Nagaraj, Bangalore Aswatha (Inventor); Williams, Jeffrey Lawrence (Inventor)

2005-01-01

An article protected by a protective coating has a substrate and a protective coating having an outer layer deposited upon the substrate surface and a diffusion zone formed by interdiffusion of the outer layer and the substrate. The protective coating includes platinum, aluminum, no more than about 2 weight percent hafnium, and substantially no silicon. The outer layer is substantially a single phase.

Alkali metal protective garment and composite material

DOEpatents

Ballif, III, John L.; Yuan, Wei W.

1980-01-01

A protective garment and composite material providing satisfactory heat resistance and physical protection for articles and personnel exposed to hot molten alkali metals, such as sodium. Physical protection is provided by a continuous layer of nickel foil. Heat resistance is provided by an underlying backing layer of thermal insulation. Overlying outer layers of fireproof woven ceramic fibers are used to protect the foil during storage and handling.

Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility.

PubMed

Gül, Aziz; Şahinler, Nuray; Onal, Ali G; Hopkins, Brandon K; Sheppard, Walter S

2017-10-01

Cryopreservation is an advanced method used to protect germplasm in liquid nitrogen. Honey bees are of special interest to protect because of their pollination activity and critical role in agriculture. There has been important progress in the cryopreservation of honey bee germplasm in recent years, leading to practical recovery of genetic material for breeding purposes following freezing. However, there remains room for improvement and the goal of the present study was to evaluate the effect of different "extenders" added post-thaw on the fertilization rate of cryopreserved honey bee semen. The purpose of adding extender post-thaw was to dilute the cryoprotectant to remove chemicals after centrifugation because of potential adverse effects. The control consisted of frozen-thawed semen without the addition of an extender; treatment groups included the addition of one of the following extenders: glucose solution, fresh ram semen plasma, fresh honey bee semen plasma, extender solution. All of the above treatments and frozen-thawed control were compared to fresh semen. For each group, 15 virgin queens were instrumentally inseminated with the semen-diluent solution and introduced into nucleus colonies to determine the brood patterns of the queens. Percentages of worker brood produced in the fresh semen, frozen-thawed semen control, glucose, fresh ram semen plasma, fresh honey bee semen plasma, and extender solution supplemented groups were 98.±1.1%, 47.0 ± 0.9%, 3.0 ± 0.8%, 0.3 ± 0.1%, 48.1 ± 4.1% and 40.3 ± 2.4%, respectively. Similiarly, spermatozoa numbers in the spermathecae of the same treatment groups were 3.6 × 10 6 , 1.6 × 10 6 , 7.3 × 10 5 , 4.7 × 10 5 , 8.1 × 10 5 , and 4.6 × 10 5 spermatozoa for the same treatment, respectively. The differences in both worker brood percentage and sperm count in the spermatheca were statistically significant (P < 0.01) among all treatment groups, except the frozen-thawed control group and fresh drone semen plasma group. We found a positive correlation between sperm count in the spermatheca and the percentage of worker brood (r = 0.91). With the exception of fresh honey bee semen plasma, the fertility rate was reduced following the addition of various plasmas and diluents post-freezing. Copyright © 2017 Elsevier Inc. All rights reserved.

European Foundation Designs for Seasonally Frozen Ground

DTIC Science & Technology

1992-03-01

104 173. Design thermal conductivity of mineral wool for frost protection...inside of theo - foundation wall, consisted of 50 mm of Rockwool o3 wallo -"o 0 ° (a mineral wool ). This insulation guides heat from the house down...Thermal Insulation Laboratory 1982.) by introducing mineral wool (Fig. 49c) gives a Table 4. Relationship between floor surface tempera- further slight

Permafrost degradation stimulates carbon loss from experimentally warmed tundra

Treesearch

S.M. Natali; E.A.G. Schuur; E. Webb; C.E. Hicks Pries; K.G. Crummer

2014-01-01

A large pool of organic carbon (C) has been accumulating in the Arctic for thousands of years because cold and waterlogged conditions have protected soil organic material from microbial decomposition. As the climate warms this vast and frozen C pool is at risk of being thawed, decomposed, and released to the atmosphere as greenhouse gasses. At the same time, some C...

Fabrication and performance of polymer-nanocomposite anti-reflective thin films deposited by RIR-MAPLE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Singaravelu, S.; Mayo, D. C.; Park, H-. K.

2014-07-01

Design of polymer anti-reflective (AR) optical coatings for plastic substrates is challenging because polymers exhibit a relatively narrow range of refractive indices. Here, we report synthesis of a four-layer AR stack using hybrid polymer: nanoparticle materials deposited by resonant infrared matrix-assisted pulsed laser evaporation. An Er: YAG laser ablated frozen solutions of a high-index composite containing TiO2 nanoparticles and poly(methylmethacrylate) (PMMA), alternating with a layer of PMMA. The optimized AR coatings, with thicknesses calculated using commercial software, yielded a coating for polycarbonate with transmission over 97 %, scattering <3 %, and a reflection coefficient below 0.5 % across the visiblemore » range, with a much smaller number of layers than would be predicted by a standard thin film calculation. The TiO2 nanoparticles contribute more to the enhanced refractive index of the high-index layers than can be accounted for by an effective medium model of the nanocomposite.« less

Cryopreservation of mouse spermatozoa. I. Effect of seeding on fertilizing ability of cryopreserved spermatozoa.

PubMed

Songsasen, N; Leibo, S P

1997-11-01

To examine the effect of seeding to induce ice formation during cryopreservation on their survival, spermatozoa from B6D2F1 mice were cooled to and held at -4 degrees C for 30 min in phosphate-buffered saline (PBS) alone, in egg yolk-supplemented PBS, or in PBS with raffinose + glycerol as cryoprotective additives (CPAs). Seeding and holding spermatozoa at -4 degrees C did not affect their viability as judged by vital staining. Egg yolk protected spermatozoa against chilling injury, as cooling them to -4 degrees C in the presence of egg yolk yielded higher survivals than those cooled without egg yolk (34.4 +/- 3.4 v 9.0 +/- 1.3% in three replicates of >400 spermatozoa/replicate). To study effects of seeding on their fertilizing ability, spermatozoa in the raffinose-glycerol-egg yolk solution were frozen to -196 degrees C either without seeding or after seeding at -4 degrees C. Development of 222 oocytes into two-cell embryos after in vitro fertilization (IVF) with spermatozoa frozen without seeding was 43%; development rates of 186, 186, and 207 oocytes after IVF with spermatozoa frozen after seeding and being held at -4 degrees C for 5, 10, or 30 min were 46, 44, and 9%, respectively. In a direct comparison, after IVF with seeded or unseeded spermatozoa the respective cleavage rates into two-cell embryos were 83% of 275 oocytes and 69% of 304 oocytes, a difference that was small but significant by chi2 analysis. An additional 925 oocytes were fertilized with spermatozoa after being seeded and frozen to -196 degrees C in four separate batches of CPA solutions. Overall, after IVF with frozen sperm, 68% of those oocytes cleaved into two-cell embryos and 59% developed into 544 blastocysts. Based on these results, we concluded that embryo production by IVF seemed to be improved using spermatozoa frozen after being seeded. Mouse spermatozoa cryopreserved by the method described here are capable of fertilizing oocytes at a rather high rate. Copyright 1997 Academic Press.

Eighteen-Year Cryopreservation Does Not Negatively Affect the Pluripotency of Human Embryos: Evidence from Embryonic Stem Cell Derivation

PubMed Central

Rungsiwiwut, Ruttachuk; Numchaisrika, Pranee; Ahnonkitpanit, Vichuda; Isarasena, Nipan; Virutamasen, Pramuan

2012-01-01

Abstract Human embryonic stem (hES) cells are considered to be a potential source for the therapy of human diseases, drug screening, and the study of developmental biology. In the present study, we successfully derived hES cell lines from blastocysts developed from frozen and fresh embryos. Seventeen- to eighteen-year-old frozen embryos were thawed, cultured to the blastocyst stage, and induced to form hES cells using human foreskin fibroblasts. The Chula2.hES cell line and the Chula4.hES and Chula5.hES cell lines were derived from blastocysts developed from frozen and fresh embryos, respectively. The cell lines expressed pluripotent markers, including alkaline phosphatase (AP), Oct3/4, stage-specific embryonic antigen (SSEA)-4, and tumor recognition antigen (TRA)-1-60 and TRA-1-81 as detected with immunocytochemistry. The real-time polymerase chain reaction (RT-PCR) results showed that the cell lines expressed pluripotent genes, including OCT3/4, SOX2, NANOG, UTF, LIN28, REX1, NODAL, and E-Cadherin. In addition, the telomerase activities of the cell lines were higher than in the fibroblast cells. Moreover, the cell lines differentiated into all three germ layers both in vitro and in vivo. The cell lines had distinct identities, as revealed with DNA fingerprinting, and maintained their normal karyotype after a long-term culture. This study is the first to report the successful derivation of hES cell lines in Thailand and that frozen embryos maintained their pluripotency similar to fresh embryos, as shown by the success of hES cell derivation, even after years of cryopreservation. Therefore, embryos from prolonged cryopreservation could be an alternative source for embryonic stem cell research. PMID:23514952

The impacts of recent permafrost thaw on land-atmosphere greenhouse gas exchange

USGS Publications Warehouse

Hayes, Daniel J.; Kicklighter, David W.; McGuire, A. David; Chen, Min; Zhuang, Qianlai; Yuan, Fengming; Melillo, Jerry M.; Wullschleger, Stan D.

2014-01-01

Permafrost thaw and the subsequent mobilization of carbon (C) stored in previously frozen soil organic matter (SOM) have the potential to be a strong positive feedback to climate. As the northern permafrost region experiences as much as a doubling of the rate of warming as the rest of the Earth, the vast amount of C in permafrost soils is vulnerable to thaw, decomposition and release as atmospheric greenhouse gases. Diagnostic and predictive estimates of high-latitude terrestrial C fluxes vary widely among different models depending on how dynamics in permafrost, and the seasonally thawed 'active layer' above it, are represented. Here, we employ a process-based model simulation experiment to assess the net effect of active layer dynamics on this 'permafrost carbon feedback' in recent decades, from 1970 to 2006, over the circumpolar domain of continuous and discontinuous permafrost. Over this time period, the model estimates a mean increase of 6.8 cm in active layer thickness across the domain, which exposes a total of 11.6 Pg C of thawed SOM to decomposition. According to our simulation experiment, mobilization of this previously frozen C results in an estimated cumulative net source of 3.7 Pg C to the atmosphere since 1970 directly tied to active layer dynamics. Enhanced decomposition from the newly exposed SOM accounts for the release of both CO2 (4.0 Pg C) and CH4 (0.03 Pg C), but is partially compensated by CO2 uptake (0.3 Pg C) associated with enhanced net primary production of vegetation. This estimated net C transfer to the atmosphere from permafrost thaw represents a significant factor in the overall ecosystem carbon budget of the Pan-Arctic, and a non-trivial additional contribution on top of the combined fossil fuel emissions from the eight Arctic nations over this time period.

78 FR 2946 - United States Standards for Grades of Frozen Vegetables

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-15

... these proposed revisions are: frozen asparagus, frozen lima beans, frozen speckled butter beans, frozen... document. United States Standards for Grades of Frozen Lima Beans Update address for AMS. Change ``U.S... obtain color standards for frozen lima beans. United States Standards for Grades of Frozen Mixed...

The usage of phase change materials in fire fighter protective clothing: its effect on thermal protection

NASA Astrophysics Data System (ADS)

Zhao, Mengmeng

2017-12-01

The thermal protective performance of the fire fighter protective clothing is of vital importance for fire fighters. In the study fabrics treated by phase change materials (PCMs) were applied in the multi-layered fabrics of the fire fighter protective clothing ensemble. The PCM fabrics were placed at the different layers of the clothing and their thermal protective performance were measured by a TPP tester. Results show that with the application of the PCM fabrics the thermal protection of the multi-layered fabrics was greatly increased. The time to reach a second degree burn was largely reduced. The location of the PCM fabrics at the different layers did not affect much on the thermal protective performance. The higher amount of the PCM adds on, the higher thermal protection was brought. The fabrics with PCMs of a higher melting temperature could contribute to higher thermal protection.

Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination

PubMed Central

Lieberman, Daniel; McClure, Elizabeth; Harston, Stephen; Madan, Damian

2016-01-01

Artificial insemination of dairy cattle is a common practice in the developing world that can improve farmer incomes and food security. Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and stored is crucial to the success of this process. Here we describe simple technological improvements to protect semen from inadvertent thermal fluctuations that occur when users mishandle semen using standard equipment. We show that when frozen semen is mishandled, characteristics of semen biology associated with fertility are negatively affected. We describe several design modifications and results from thermal performance tests of several improved prototypes. Finally, we compare semen that has been mishandled in standard and improved equipment. The data suggest that our canister improvements can better maintain characteristics of semen biology that correlate with fertility when it is mishandled. PMID:27313137

Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination.

PubMed

Lieberman, Daniel; McClure, Elizabeth; Harston, Stephen; Madan, Damian

2016-06-17

Artificial insemination of dairy cattle is a common practice in the developing world that can improve farmer incomes and food security. Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and stored is crucial to the success of this process. Here we describe simple technological improvements to protect semen from inadvertent thermal fluctuations that occur when users mishandle semen using standard equipment. We show that when frozen semen is mishandled, characteristics of semen biology associated with fertility are negatively affected. We describe several design modifications and results from thermal performance tests of several improved prototypes. Finally, we compare semen that has been mishandled in standard and improved equipment. The data suggest that our canister improvements can better maintain characteristics of semen biology that correlate with fertility when it is mishandled.

Maintaining semen quality by improving cold chain equipment used in cattle artificial insemination

NASA Astrophysics Data System (ADS)

Lieberman, Daniel; McClure, Elizabeth; Harston, Stephen; Madan, Damian

2016-06-01

Artificial insemination of dairy cattle is a common practice in the developing world that can improve farmer incomes and food security. Maintaining the fertilizing potential of frozen semen as it is manipulated, transported and stored is crucial to the success of this process. Here we describe simple technological improvements to protect semen from inadvertent thermal fluctuations that occur when users mishandle semen using standard equipment. We show that when frozen semen is mishandled, characteristics of semen biology associated with fertility are negatively affected. We describe several design modifications and results from thermal performance tests of several improved prototypes. Finally, we compare semen that has been mishandled in standard and improved equipment. The data suggest that our canister improvements can better maintain characteristics of semen biology that correlate with fertility when it is mishandled.

Permafrost Meta-Omics and Climate Change

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mackelprang, Rachel; Saleska, Scott R.; Jacobsen, Carsten Suhr

2016-06-29

Permafrost (i.e., soil that has been frozen for at least 2 consecutive years) represents a habitat for microbial life at subzero temperatures (Gilichinsky et al. 2008). Approximately one quarter of the Earth’s surface is underlain by permafrost, which contains 25-50% of the total global soil carbon pool (Schuur et al. 2008, Tarnocai et al. 2009). This carbon is largely protected from microbial decomposition by reduced microbial activity in frozen conditions, but climate change is threatening to induce large-scale permafrost thaw thus exposing it to degradation. The resulting emissions of greenhouse gasses (GHGs) can produce a positive feedback loop and significantlymore » amplify the effects of global warming. Increasing temperatures at high latitudes, changes in precipitation patterns, and frequent fire events have already initiated a widespread degradation of permafrost (Schuur et al. 2015).« less

Preliminary Guidelines and Standard Operating Procedure for Drainage and Erosion Control at McMurdo Station

DTIC Science & Technology

2014-12-01

along the impermeable frozen soil layer. Soil freeze–thaw action disrupts soil structures, displaces soils particles , and creates voids both in...discharging along the bank face). This was caused by excess pore water pressure. Soil piping removes soil particles from their in-situ position, leaving...soil particles ERDC/CRREL SR-14-3 30 c. Maintain original side and bed slopes during the clearing efforts. d. Shape the channels to minimize

Moessbauer spectroscopy analysis of {sup 57}Fe-doped YBaCo{sub 4}O{sub 7+{delta}}: Effects of oxygen intercalation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tsipis, E.V.; Department of Ceramics and Glass Engineering, CICECO, University of Aveiro, 3810-193 Aveiro; Waerenborgh, J.C.

2009-03-15

Moessbauer spectroscopy of layered YBaCo{sub 3.96}Fe{sub 0.04}O{sub 7+{delta}} ({delta}=0.02 and 0.80), where 1% cobalt is substituted with {sup 57}Fe isotope, revealed no evidence of charge ordering at 4-293 K. The predominant state of iron cations was found trivalent, irrespective of their coordination and oxygen stoichiometry variations determined by thermogravimetric analysis. The extremely slow kinetics of isothermal oxidation at 598 K in air, and the changes of Fe{sup 3+} fractions in the alternating triangular and Kagome layers in oxidized YBaCo{sub 3.96}Fe{sub 0.04}O{sub 7.80}, may suggest that oxygen intercalation is accompanied with a substantial structural reconstruction stagnated due to sluggish cation diffusion.more » Decreasing temperature below 75-80 K leads to gradual freezing of the iron magnetic moments in inverse correlation with the content of extra oxygen. The formation of metal-oxygen octahedra and resultant structural distortions extend the temperature range where the paramagnetic and frozen states co-exist, down to 45-50 K. - Graphical abstract: Moessbauer spectroscopy of layered YBaCo{sub 3.96}Fe{sub 0.04}O{sub 7+{delta}} ({delta}=0.02 and 0.80), with 1% {sup 57}Fe isotope substituted for cobalt, revealed no evidence of charge ordering at 4-293 K. The predominant state of iron cations was found trivalent, irrespective of their coordination and oxygen stoichiometry variations determined by thermogravimetric analysis. Decreasing temperature below 75-80 K leads to gradual freezing of the iron magnetic moments in inverse correlation with the content of extra oxygen extending the temperature range where the paramagnetic and frozen states co-exist down to 45-50 K.« less

Snow Water Equivalent Retrieval By Markov Chain Monte Carlo Based on Memls and Hut Snow Emission Model

NASA Astrophysics Data System (ADS)

Pan, J.; Durand, M. T.; Vanderjagt, B. J.

2014-12-01

The Markov chain Monte Carlo (MCMC) method had been proved to be successful in snow water equivalent retrieval based on synthetic point-scale passive microwave brightness temperature (TB) observations. This method needs only general prior information about distribution of snow parameters, and could estimate layered snow properties, including the thickness, temperature, density and snow grain size (or exponential correlation length) of each layer. In this study, the multi-layer HUT (Helsinki University of Technology) model and the MEMLS (Microwave Emission Model of Layered Snowpacks) will be used as observation models to assimilate the observed TB into snow parameter prediction. Previous studies had shown that the multi-layer HUT model tends to underestimate TB at 37 GHz for deep snow, while the MEMLS does not show sensitivity of model bias to snow depth. Therefore, results using HUT model and MEMLS will be compared to see how the observation model will influence the retrieval of snow parameters. The radiometric measurements at 10.65, 18.7, 36.5 and 90 GHz at Sodankyla, Finland will be used as MCMC input, and the statistics of all snow property measurement will be used to calculate the prior information. 43 dry snowpits with complete measurements of all snow parameters will be used for validation. The entire dataset are from NorSREx (Nordic Snow Radar Experiment) experiments carried out by Juha Lemmetyinen, Anna Kontu and Jouni Pulliainen in FMI in 2009-2011 winters, and continued two more winters from 2011 to Spring of 2013. Besides the snow thickness and snow density that are directly related to snow water equivalent, other parameters will be compared with observations, too. For thin snow, the previous studies showed that influence of underlying soil is considerable, especially when the soil is half frozen with part of unfrozen liquid water and part of ice. Therefore, this study will also try to employ a simple frozen soil permittivity model to improve the performance of retrieval. The behavior of the Markov chain in soil parameters will be studied.

Parametric study of solar thermal rocket nozzle performance

NASA Technical Reports Server (NTRS)

Pearson, J. Boise; Landrum, D. Brian; Hawk, Clark W.

1995-01-01

This paper details a numerical investigation of performance losses in low-thrust solar thermal rocket nozzles. The effects of nozzle geometry on three types of losses were studied; finite rate dissociation-recombination kinetic losses, two dimensional axisymmetric divergence losses, and compressible viscous boundary layer losses. Short nozzle lengths and supersonic flow produce short residence times in the nozzle and a nearly frozen flow, resulting in large kinetic losses. Variations in geometry have a minimal effect on kinetic losses. Divergence losses are relatively small, and careful shaping of the nozzle can nearly eliminate them. The boundary layer in these small nozzles can grow to a major fraction of nozzle radius, and cause large losses. These losses are attributed to viscous drag on the nozzle walls and flow blockage by the boundary layer, especially in the throat region. Careful shaping of the nozzle can produce a significant reduction in viscous losses.

Spacecraft ceramic protective shield

NASA Technical Reports Server (NTRS)

Larriva, Rene F. (Inventor); Nelson, Anne (M.); Czechanski, James G. (Inventor); Poff, Ray E. (Inventor)

1995-01-01

A low areal density protective shield apparatus, and method for making same, for protecting spacecraft structures from impact with hypervelocity objects, including a bumper member comprising a bumper ceramic layer, a bumper shock attenuator layer, and a bumper confining layer. The bumper ceramic layer can be SiC or B.sub.4 C; the bumper shock attenuator layer can be zirconia felt; and the bumper confining layer can be aluminum. A base armor member can be spaced from the bumper member and a ceramic fiber-based curtain can be positioned between the bumper and base armor members.

[In vitro activity of human bone marrow cells after cryopreservation in liquid nitrogen for 21 - 25 years].

PubMed

Huang, You-Zhang; Shen, Jian-Liang; Gong, Li-Zhong; Zheng, Pei-Hao; Liu, Yi; Yin, Wen-Jie; Cen, Jian; Wang, Ning; Zhao, De-Feng

2010-02-01

The aim of this study was to investigate the best method to preserve human bone marrow cells and the effectiveness of long term cryopreservation at -80 degrees C. The human bone marrow cells in 20 samples were firstly frozen by a programmed freezer or -80 degrees C refrigerator, and then were preserved in liquid nitrogen with DMSO-AuP (10% dimethylsulfonamide, 10% autologous plasma) or DMSO-HES-HuA (5% dimethylsulfonamide, 6% hydroxyethyl starch, 4% human serum albumin) as cryoprotectant for 21 to 25 years. They were thawed in 38 degrees C. The cell sample frozen in -80 degrees C refrigerator was frozen at a low frozen speed of 1 degrees C/min which was the same as the programmed freezer before -30 degrees C. Before detection the bone marrow cells were taken from liquid nitrogen and were thawed in 38 degrees C, then the suspension of bone marrow cells was prepared for detection. The cell morphology and recovery rate of erythrocytes, nucleocytes and platelets; the recovery rate of hematopoietic stem progenitors cells, as well as mesenchymal stem cells were determined. The results showed that the protective effectiveness of DMSO-HES-HuA was better than DMSO-AuP. The mature erythrocytes were destroyed lightly [(3.5 +/- 1.5)% versus (12.6 +/- 4.8)%], the hemolysis rate was lower [(3.3 +/- 1.6)% versus (23.1 +/- 5.1)%]. Osmotic fragility of erythrocytes in the former was not changed, but was dropped in the latter. The recovery rates of red cell, platelet, granulocyte-macrophage colony forming units and long term culture-initiating cells were higher in the former than that in the latter [(96.1 +/- 1.8)%, (70.0 +/- 9.5)%, (49.2 +/- 10.9)%, (54.2 +/- 13.8)% versus (76.3 +/- 5.6)%, (52.7 +/- 8.1)%, (43.5 +/- 12.3)%, (47.2 +/- 13.6)% respectively]. With each kind of cryoprotectant or frozen method, the frozen MSC could keep the original growth properties. With the same cryoprotectant and different frozen method, the cryopreservative effectiveness was not different. The influence of the cryoprotectant prescriptions and the frozen methods on the cryopreservative effectiveness was little. It is concluded that the human bone marrow cells with DMSO-AuP or DMSO-HES-HuA as cryoprotectant, frozen by a programmed freezer or -80 degrees C refrigerator, could be then preserved in liquid nitrogen for long time. When the preserving time was as long as 21 to 25 years, the morphology, the recovery rate and the activity of various kinds of cells were still good. The method of freezing by -80 degrees C refrigerator with 5% DMSO-6% HES-4% HuA and preserving in liquid nitrogen would be convenient, cheap and easily-manipulated for preservation of the human bone marrow cells.

Degradation of microbial fluorescence biosignatures by solar ultraviolet radiation on Mars

NASA Astrophysics Data System (ADS)

Dartnell, Lewis R.; Patel, Manish R.

2014-04-01

Recent and proposed robotic missions to Mars are equipped with implements to expose or excavate fresh material from beneath the immediate surface. Once brought into the open, any organic molecules or potential biosignatures of present or past life will be exposed to the unfiltered solar ultraviolet (UV) radiation and face photolytic degradation over short time courses. The key question, then, is what is the window of opportunity for detection of recently exposed samples during robotic operations? Detection of autofluorescence has been proposed as a simple method for surveying or triaging samples for organic molecules. Using a Mars simulation chamber we conduct UV exposures on thin frozen layers of two model microorganisms, the radiation-resistant polyextremophile Deinococcus radiodurans and the cyanobacterium Synechocystis sp. PCC 6803. Excitation-emission matrices (EEMs) are generated of the full fluorescence response to quantify the change in signal of different cellular fluorophores over Martian equivalent time. Fluorescence of Deinococcus cells, protected by a high concentration of carotenoid pigments, was found to be relatively stable over 32 h of Martian UV irradiation, with around 90% of the initial signal remaining. By comparison, fluorescence from protein-bound tryptophan in Synechocystis is much more sensitive to UV photodegradation, declining to 50% after 64 h exposure. The signal most readily degraded by UV irradiation is fluorescence of the photosynthetic pigments - diminished to only 35% after 64 h. This sensitivity may be expected as the biological function of chlorophyll and phycocyanin is to optimize the harvesting of light energy and so they are readily photobleached. A significant increase in a ~450 nm emission feature is interpreted as accumulation of fluorescent cellular degradation products from photolysis. Accounting for diurnal variation in Martian sunlight, this study calculates that frozen cellular biosignatures would remain detectable by fluorescence for at least several sols; offering a sufficient window for robotic exploration operations.

Method of depositing a protective layer over a biaxially textured alloy substrate and composition therefrom

DOEpatents

Goyal, Amit; Kroeger, Donald M.; Paranthaman, Mariappan; Lee, Dominic F.; Feenstra, Roeland; Norton, David P.

2002-01-01

A laminate article consists of a substrate and a biaxially textured protective layer over the substrate. The substrate can be biaxially textured and also have reduced magnetism over the magnetism of Ni. The substrate can be selected from the group consisting of nickel, copper, iron, aluminum, silver and alloys containing any of the foregoing. The protective layer can be selected from the group consisting of gold, silver, platinum, palladium, and nickel and alloys containing any of the foregoing. The protective layer is also non-oxidizable under conditions employed to deposit a desired, subsequent oxide buffer layer. Layers of YBCO, CeO.sub.2, YSZ, LaAlO.sub.3, SrTiO.sub.3, Y.sub.2 O.sub.3, RE.sub.2 O.sub.3, SrRuO.sub.3, LaNiO.sub.3 and La.sub.2 ZrO.sub.3 can be deposited over the protective layer. A method of forming the laminate article is also disclosed.

Biocide squirting from an elastomeric tri-layer film.

PubMed

Sonntag, Philippe; Hoerner, Pierre; Cheymol, André; Argy, Gilles; Riess, Gérard; Reiter, Günter

2004-05-01

Protective layers typically act in a passive way by simply separating two sides. Protection is only efficient as long as the layers are intact. If a high level of protection has to be achieved by thin layers, complementary measures need to be in place to ensure safety, even after breakage of the layer-an important issue in medical applications. Here, we present a novel approach for integrating a biocide liquid into a protective film (about 300-500 microm thick), which guarantees that a sufficient amount of biocide is rapidly released when the film is punctured. The film is composed of a middle layer, containing the liquid in droplet-like compartments, sandwiched between two elastomeric boundary layers. When the film is punctured, the liquid squirts out of the middle layer. A theoretical model was used to determine the size and density of droplets that are necessary to ensure a sufficient quantity of biocide is expelled from an adequately elastic matrix to provide protection at the site of damage. We demonstrate the utility of this approach for the fabrication of surgical gloves.

'Scaling' analysis of the ice accretion process on aircraft surfaces

NASA Technical Reports Server (NTRS)

Keshock, E. G.; Tabrizi, A. H.; Missimer, J. R.

1982-01-01

A comprehensive set of scaling parameters is developed for the ice accretion process by analyzing the energy equations of the dynamic freezing zone and the already frozen ice layer, the continuity equation associated with supercooled liquid droplets entering into and impacting within the dynamic freezing zone, and energy equation of the ice layer. No initial arbitrary judgments are made regarding the relative magnitudes of each of the terms. The method of intrinsic reference variables in employed in order to develop the appropriate scaling parameters and their relative significance in rime icing conditions in an orderly process, rather than utilizing empiricism. The significance of these parameters is examined and the parameters are combined with scaling criteria related to droplet trajectory similitude.

Perspective of Life Search in Martian Econiches

NASA Astrophysics Data System (ADS)

Demidov, N. E.

2017-05-01

Mars may be divided on five ecological niches according to presence and state of water: permanent polar caps, dry regolith, subpermafrost aquifers, cryopegs and ice containing regolith. Basic limiting factors for the search of life in this econiches are: absence of water (dry regolith), depth of burial (cryopegs and subpermafrost aquifers), age (ice containing permafrost and polar caps). High priority targets for the search of life on Mars are represented by permanently frozen deposits of young polar volcanoes and ash layers in polar caps. During volcanic eruptions microorganisms from subpermafrost aquifers could propagate to the surface and survive in permafrost or ice for million years, as it is known to happen on Earth. Possibility of specific lithic habitats in dry layer must also be taken into account.

Standard deviation of vertical two-point longitudinal velocity differences in the atmospheric boundary layer.

NASA Technical Reports Server (NTRS)

Fichtl, G. H.

1971-01-01

Statistical estimates of wind shear in the planetary boundary layer are important in the design of V/STOL aircraft, and for the design of the Space Shuttle. The data analyzed in this study consist of eleven sets of longitudinal turbulent velocity fluctuation time histories digitized at 0.2 sec intervals with approximately 18,000 data points per time history. The longitudinal velocity fluctuations were calculated with horizontal wind and direction data collected at the 18-, 30-, 60-, 90-, 120-, and 150-m levels. The data obtained confirm the result that Eulerian time spectra transformed to wave-number spectra with Taylor's frozen eddy hypothesis possess inertial-like behavior at wave-numbers well out of the inertial subrange.

Chemistry in protoplanetary disks

NASA Astrophysics Data System (ADS)

Semenov, D. A.

2012-01-01

In this lecture I discuss recent progress in the understanding of the chemical evolution of protoplanetary disks that resemble our Solar system during the first ten million years. At the verge of planet formation, strong variations of temperature, density, and radiation intensities in these disks lead to a layered chemical structure. In hot, dilute and heavily irradiated atmosphere only simple radicals, atoms, and atomic ions can survive, formed and destroyed by gas-phase processes. Beneath the atmosphere a partly UV-shielded, warm molecular layer is located, where high-energy radiation drives rich chemistry, both in the gas phase and on dust surfaces. In a cold, dense, dark disk midplane many molecules are frozen out, forming thick icy mantles where surface chemistry is active and where complex (organic) species are synthesized.

Penetration and decontamination of americium-241 ex vivo using fresh and frozen pig skin.

PubMed

Tazrart, A; Bolzinger, M A; Moureau, A; Molina, T; Coudert, S; Angulo, J F; Briancon, S; Griffiths, N M

2017-04-01

Skin contamination is one of the most probable risks following major nuclear or radiological incidents. However, accidents involving skin contamination with radionuclides may occur in the nuclear industry, in research laboratories and in nuclear medicine departments. This work aims to measure the penetration of the radiological contaminant Americium ( 241 Am) in fresh and frozen skin and to evaluate the distribution of the contamination in the skin. Decontamination tests were performed using water, Fuller's earth and diethylene triamine pentaacetic acid (DTPA), which is the recommended treatment in case of skin contamination with actinides such as plutonium or americium. To assess these parameters, we used the Franz cell diffusion system with full-thickness skin obtained from pigs' ears, representative of human skin. Solutions of 241 Am were deposited on the skin samples. The radioactivity content in each compartment and skin layers was measured after 24 h by liquid scintillation counting and alpha spectrophotometry. The Am cutaneous penetration to the receiver compartment is almost negligible in fresh and frozen skin. Multiple washings with water and DTPA recovered about 90% of the initial activity. The rest remains fixed mainly in the stratum corneum. Traces of activity were detected within the epidermis and dermis which is fixed and not accessible to the decontamination. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Review of Thermosyphon Applications

DTIC Science & Technology

2014-02-11

method (Johnston 1981) is typically used to protect permafrost. A passive method maintains the frozen state of the soil. The most widely used passive...technique is to incorporate ventilated air spaces beneath elevated buildings (Shur and Goering 2009). This method is recommended for locations with...permafrost temperatures be- low –3°C (Johnston 1981) and provides a measure of thermal stability. Ac- tive methods concentrate on removing the thaw

Freeze-thaw stress of Alhydrogel ® alone is sufficient to reduce the immunogenicity of a recombinant hepatitis B vaccine containing native antigen.

PubMed

Clapp, Tanya; Munks, Michael W; Trivedi, Ruchit; Kompella, Uday B; Braun, LaToya Jones

2014-06-24

Preventing losses in vaccine potency due to accidental freezing has recently become a topic of interest for improving vaccines. All vaccines with aluminum-containing adjuvants are susceptible to such potency losses. Recent studies have described excipients that protect the antigen from freeze-induced inactivation, prevent adjuvant agglomeration and retain potency. Although these strategies have demonstrated success, they do not provide a mechanistic understanding of freeze-thaw (FT) induced potency losses. In the current study, we investigated how adjuvant frozen in the absence of antigen affects vaccine immunogenicity and whether preventing damage to the freeze-sensitive recombinant hepatitis B surface antigen (rHBsAg) was sufficient for maintaining vaccine potency. The final vaccine formulation or Alhydrogel(®) alone was subjected to three FT-cycles. The vaccines were characterized for antigen adsorption, rHBsAg tertiary structure, particle size and charge, adjuvant elemental content and in-vivo potency. Particle agglomeration of either vaccine particles or adjuvant was observed following FT-stress. In vivo studies demonstrated no statistical differences in IgG responses between vaccines with FT-stressed adjuvant and no adjuvant. Adsorption of rHBsAg was achieved; regardless of adjuvant treatment, suggesting that the similar responses were not due to soluble antigen in the frozen adjuvant-containing formulations. All vaccines with adjuvant, including the non-frozen controls, yielded similar, blue-shifted fluorescence emission spectra. Immune response differences could not be traced to differences in the tertiary structure of the antigen in the formulations. Zeta potential measurements and elemental content analyses suggest that FT-stress resulted in a significant chemical alteration of the adjuvant surface. This data provides evidence that protecting a freeze-labile antigen from subzero exposure is insufficient to maintain vaccine potency. Future studies should focus on adjuvant protection. To our knowledge, this is the first study to systematically investigate how FT-stress to adjuvant alone affects immunogenicity. It provides definitive evidence that this damage is sufficient to reduce vaccine potency. Copyright © 2014 Elsevier Ltd. All rights reserved.

Study on the Weak Stress in Flexural MEMS Cantilever

NASA Astrophysics Data System (ADS)

Ge, Yuetao; Ren, Yan

2018-03-01

In order to design a better piezoresistive MEMS cantilever beam, especially for cantilever beams that will detect weak forces or will be subjected to weak forces, this paper uses study on the weak stress in flexural MEMS cantilever. The sensor design structure, divided into protective layer, piezoresistive layer, support layer. The protective layer is responsible for protecting the piezoresistive layer so that the varistor is insulated from the outside; the piezoresistive layer is used to make the varistor; the support layer forms the main part of the cantilever beam, the majority of the cantilever beam. This paper has some value for cantilever multilayer structure design and cantilever beam size design.

Conventional freezing plus high pressure-low temperature treatment: Physical properties, microbial quality and storage stability of beef meat.

PubMed

Fernández, Pedro P; Sanz, Pedro D; Molina-García, Antonio D; Otero, Laura; Guignon, Bérengère; Vaudagna, Sergio R

2007-12-01

Meat high-hydrostatic pressure treatment causes severe decolouration, preventing its commercialisation due to consumer rejection. Novel procedures involving product freezing plus low-temperature pressure processing are here investigated. Room temperature (20°C) pressurisation (650MPa/10min) and air blast freezing (-30°C) are compared to air blast freezing plus high pressure at subzero temperature (-35°C) in terms of drip loss, expressible moisture, shear force, colour, microbial quality and storage stability of fresh and salt-added beef samples (Longissimus dorsi muscle). The latter treatment induced solid water transitions among ice phases. Fresh beef high pressure treatment (650MPa/20°C/10min) increased significantly expressible moisture while it decreased in pressurised (650MPa/-35°C/10min) frozen beef. Salt addition reduced high pressure-induced water loss. Treatments studied did not change fresh or salt-added samples shear force. Frozen beef pressurised at low temperature showed L, a and b values after thawing close to fresh samples. However, these samples in frozen state, presented chromatic parameters similar to unfrozen beef pressurised at room temperature. Apparently, freezing protects meat against pressure colour deterioration, fresh colour being recovered after thawing. High pressure processing (20°C or -35°C) was very effective reducing aerobic total (2-log(10) cycles) and lactic acid bacteria counts (2.4-log(10) cycles), in fresh and salt-added samples. Frozen+pressurised beef stored at -18°C during 45 days recovered its original colour after thawing, similarly to just-treated samples while their counts remain below detection limits during storage.

Microbiological examination of ready-to-eat foods and ready-to-bake frozen pastries from university canteens.

PubMed

Kotzekidou, Parthena

2013-06-01

During a 10-year inspection survey (2001-2010), a microbiological study of ready-to-eat (RTE) foods and ready-to-bake frozen pastries from 15 canteens of the university campus was undertaken to determine their microbiological quality. The cumulative study revealed that the aerobic colony counts for the RTE product groups were as follows: from 10(6) to 10(8) CFU/g for 50% of sandwiches; under the detection limit (<10 CFU/g) for 88.6% of oven baked pastries; <10(5) CFU/g for 86.5% of desserts oven baked; from 10(3) to 10(9) CFU/g for desserts with dairy cream. The highest mean Enterobacteriaceae counts were recorded for desserts with dairy cream. The highest percentages of foodborne pathogens were: 20% Listeria monocytogenes and 12.5% Staphylococcus aureus in desserts with dairy cream; 17.5% Salmonella spp. and 8.5% presumptive Escherichia coli O157 in sandwiches; 14.6% Bacillus cereus in oven baked pastries. Aerobic colony counts were in the range 10(7)-10(8) CFU/g for 48.8% of frozen pastries; whereas Enterobacteriaceae counts between 10(3) and 10(4) CFU/g were detected in 35.3%. Foodborne pathogens prevalences for frozen pastries were as follows: B. cereus, 31.8%; Salmonella spp., 28.6%; presumptive E. coli O157, 25%; S. aureus, 8.7%; L. monocytogenes, 8.7%. Improved sanitary conditions in the processing plants and precautionary measures are necessary for consumer protection. Copyright © 2013 Elsevier Ltd. All rights reserved.

The role of the cubic structure in freezing of a supercooled water droplet on an ice substrate

NASA Astrophysics Data System (ADS)

Takahashi, T.; Kobayashi, T.

1983-12-01

The possibility of the formation of a metastable cubic (diamond) structure and its role in freezing of a supercooled water droplet on an ice substrate are discussed in terms of two-dimensional nucleation. The mode of stacking sequence of new layers formed by two-dimensional nucleation is divided into single and multi-nucleation according to the degree of supercooling and to the size of the supercooled droplet. In the case of single nucleation a frozen droplet develops into a complete hexagonal single crystal or an optically single crystal (containing discontinuous stacking faults). In the case of multi-nucleation attention is paid to the size effect and the stacking direction of the nucleus to calculate the waiting time in the nucleation. Then the frozen droplets are crystallographically divided into three categories: completely single crystals, optically single crystals (containing a small cubic structure, i.e. stacking faults) and polycrystals with a misorientation of 70.53° between the c-axes.

Buried CO2 Ice traces in South Polar Layered Deposits of Mars detected by radar sounder

NASA Astrophysics Data System (ADS)

Castaldo, L.; Mège, D.; Orosei, R.; Séjourné, A.

2014-12-01

SHARAD (SHAllow RADar) is the subsurface sounding radar provided by the Italian Space Agency (ASI) as a facility instrument to NASA's 2005 Mars Reconnaissance Orbiter (MRO). The Reduced Data Record of SHARAD data covering the area of the South Polar Layered Deposits (SPLD), has been used. The elaboration and interpretation of the data, aimed to estimate electromagnetic properties of surface layers, has been performed in terms of permittivity. The theory of electromagnetic scattering from fractal surfaces, and the estimation of geometric parameters from topographic data by Mars Orbiter Laser Altimeter (MOLA) which was one of five instruments on board the Mars Global Surveyor (MGS) spacecraft, has been used. A deep analysis of inversion has been made on all Mars and extended to the South Polar Caps in order to extract the area with a permittivity constant of CO2 ice. Several corrections have been applied to the data, moreover the calibration of the signal requires the determination of a constant that takes into account the power gain due to the radar system and the surface in order to compensate the power losses due to the orbitographic phenomena. The determination of regions with high probability of buried CO2 ice in the first layer of the Martian surface, is obtained extracting the real part of the permittivity constant of the CO2 ice (~2), estimated by other means. The permittivity of CO2ice is extracted from the Global Permittivity Map of Mars using the global standard deviation of itself as following: ɛCO2ice=ɛCO2ice+ Σ (1)where Σ=±std(ɛMapMars)/2Figure 1(a) shows the south polar areas where the values of the permittivity point to the possibility of a CO2 ice layer. Figure 1(b) is the corresponding geologic map. The comparison between the two maps indicates that the area with probable buried CO2 overlaps Hesperian and Amazonian polar units (Hp, Hesperian plains-forming deposits marked by narrow sinuous, anabranching ridges and irregular depressions, and Apu, Amazonian layered plateaus). From this analysis, the south polar cap could be covered by a thin frozen carbon dioxide coating. The perennial south polar cap is probably made of frozen carbon dioxide ca. 8 meters thick.

Are there carbonate deposits in the Valles Marineris, Mars?

NASA Technical Reports Server (NTRS)

Nedell, Susan S.; Mckay, Christopher P.

1989-01-01

The precipitation of 30 mbar of Martian atmosphere CO2 as carbonates in lakes is suggested to be the source of thick sequences of layered deposits found in the Valles Marineris. Support is adduced for this scenario from processes occurring in the perennially frozen dry valley lakes of Antarctica, where the lake water is supersaturated with atmospheric gases. Atmospheric CO2 would have accumulated in such Martian lakes as temperature fell, and the presence of an insulating ice cover would have allowed liquid water to exist.

The Complementary Effect of Cholesterol and Vitamin E Preloaded in Cyclodextrins on Frozen Bovine Semen: Motility Parameters, Membrane Integrity and Lipid Peroxidation.

PubMed

Khellouf, A; Benhenia, K; Fatami, S; Iguer-Ouada, M

During cryopreservation sperm cells suffer from two major deleterious impacts: oxidative stress and cold shock. To investigate in bovine species the benefit of cholesterol and vitamin E, both loaded in cyclodextrins, as a double protection against oxidative stress and cold shock. Semen was collected from nine mature bulls using an artificial vagina and each ejaculate was split into four equal aliquots. The control aliquot was diluted with Fraction A (Tris+citric acid+fructose+penicillin) without further supplementation; the treated samples were diluted in Fraction A supplemented with cholesterol-loaded cyclodextrins (CD-CHL), vitamin E-loaded cyclodextrins (CD-Vit E) or CD-CHL in association with CD-Vit E (CD-CHL-VitE). After incubation at 22°C for 15 min and addition of Fraction B (Fraction A+egg yolk+glycerol), all aliquots were frozen in 0.25 ml straws. Straws were then thawed individually at 37C for 30 seconds in a water bath and immediately analyzed for motility, using Computer Aided Semen Analysis (CASA), membrane integrity and oxidative stress status. The results showed that samples treated with CD-CHL and CD-Vit E were protected against the deleterious impact of freezing thawing process. However, the optimal protection was observed when the two complexes CD-CHL and CD-Vit E were simultaneously used. All analysed semen parameters including motility, membrane integrity and oxidative stress status were significantly improved in CD-CHL-Vit E compared to all other treatments. Cholesterol and vitamin E, both preloaded in cyclodextrins to increase their solubility, appeared as a powerful protection in cryopreserved bovine semen to fight simultaneously cold shock and oxidative stress.

78 FR 55676 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Preliminary Results of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-11

...The Department of Commerce (the ``Department'') is conducting the ninth administrative review and eleventh new shipper review (``NSR'') of the antidumping duty order on certain frozen fish fillets (``fish fillets'') from the Socialist Republic of Vietnam (``Vietnam'').\\1\\ The Department has preliminarily determined that new shipper review respondent, Golden Quality Seafood Corporation (``Golden Quality''), as well as administrative review mandatory respondents, Hung Vuong Group (``HVG'') \\2\\ and Vinh Hoan Corporation (``Vinh Hoan''),\\3\\ sold subject merchandise in the United States at prices below normal value (``NV'') during the period of review (``POR'') August 1, 2011, through July 31, 2012. If these preliminary results are adopted in the final results, the Department will instruct U.S. Customs and Border Protection (``CBP'') to assess antidumping duties on all appropriate entries of subject merchandise during the POR. Interested parties are invited to comment on these preliminary results. ---------------------------------------------------------------------------

Atomic-Layer-Deposited Transparent Electrodes for Silicon Heterojunction Solar Cells

DOE PAGES

Demaurex, Benedicte; Seif, Johannes P.; Smit, Sjoerd; ...

2014-11-01

We examine damage-free transparent-electrode deposition to fabricate high-efficiency amorphous silicon/crystalline silicon heterojunction solar cells. Such solar cells usually feature sputtered transparent electrodes, the deposition of which may damage the layers underneath. Using atomic layer deposition, we insert thin protective films between the amorphous silicon layers and sputtered contacts and investigate their effect on device operation. We find that a 20-nm-thick protective layer suffices to preserve, unchanged, the amorphous silicon layers beneath. Insertion of such protective atomic-layer-deposited layers yields slightly higher internal voltages at low carrier injection levels. However, we identify the presence of a silicon oxide layer, formed during processing,more » between the amorphous silicon and the atomic-layer-deposited transparent electrode that acts as a barrier, impeding hole and electron collection.« less

Viability of Lactobacillus acidophilus in synbiotic guava mousses and its survival under in vitro simulated gastrointestinal conditions.

PubMed

Buriti, Flávia C A; Castro, Inar A; Saad, Susana M I

2010-02-28

The effects of refrigeration, freezing and substitution of milk fat by inulin and whey protein concentrate (WPC) on Lactobacillus acidophilus La-5 viability and resistance to gastric and enteric simulated conditions in synbiotic guava mousses effects were investigated. Refrigerated mousses supplemented with WPC presented the best probiotic viability, ranging from 7.77 to 6.24 log cfu/g during 28 days of storage. The highest probiotic populations, above 7.45 log cfu/g, were observed for all frozen mousses during 112 days of storage. Decreased L. acidophilus survival during the in vitro gastrointestinal simulation was observed both for refrigerated and frozen mousses. Nonetheless, for the refrigerated mousses, the addition of inulin enhanced the probiotic survival during the in vitro assays in the first week of storage. L. acidophilus survival in simulated gastrointestinal fluids was also improved through freezing. The frozen storage may be used to provide increased shelf-life for synbiotic guava mousses. Even though the protective effect of inulin and WPC on the probiotic microorganism tested was shown to be more specific for the refrigerated products, the partial replacement of milk fat by these ingredients may also help, as it improves the nutritional value of mousses in both storage conditions. (c) 2009 Elsevier B.V. All rights reserved.

Investigation on transmission and reflection characteristics of plasma array to 6 GHz high-power microwave

NASA Astrophysics Data System (ADS)

Yang, Liu; Yang, Zhongcun; Wan, Jianing; Liu, Hao

2016-10-01

For the safety of electronic equipment, a double-layer barrier of cylindrical plasma array was designed, and its protective performance to high-power microwave (HPM) were analyzed and the protective performance experiment was conducted. Combining the density distribution characteristic of the discharge plasma, the shielding effectiveness of the double-layer plasma on 6GHz HPM pulse was studied. The experiment results indicate that the protective effectiveness of two layers plasma array is better than that of one layer. Two layers plasma array can make the peak electric field of transmission waveform less than interference threshold of electronic equipment to achieve better protection effectiveness. Transmission attenuation of one layer and two layers plasma array to HPM can reach -6.6066dB and -24.9357dB. The results also show that for the existence of multiple reflection, even the plasma electron density is not high enough, it can realize a strong attenuation. The experiment results in this paper are of great significance in protecting against HPM and electromagnetic pulse.

Protecting peroxidase activity of multilayer enzyme-polyion films using outer catalase layers.

PubMed

Lu, Haiyun; Rusling, James F; Hu, Naifei

2007-12-27

Films constructed layer-by-layer on electrodes with architecture {protein/hyaluronic acid (HA)}n containing myoglobin (Mb) or horseradish peroxidase (HRP) were protected against protein damage by H2O2 by using outer catalase layers. Peroxidase activity for substrate oxidation requires activation by H2O2, but {protein/HA}n films without outer catalase layers are damaged slowly and irreversibly by H2O2. The rate and extent of damage were decreased dramatically by adding outer catalase layers to decompose H2O2. Comparative studies suggest that protection results from catalase decomposing a fraction of the H2O2 as it enters the film, rather than by an in-film diffusion barrier. The outer catalase layers controlled the rate of H2O2 entry into inner regions of the film, and they biased the system to favor electrocatalytic peroxide reduction over enzyme damage. Catalase-protected {protein/HA}n films had an increased linear concentration range for H2O2 detection. This approach offers an effective way to protect biosensors from damage by H2O2.

Apparatus and method for the electrolytic production of metals

DOEpatents

Sadoway, Donald R.

1991-01-01

Improved electrolytic cells and methods for producing metals by electrolytic reduction of a compound dissolved in a molten electrolyte are disclosed. In the improved cells and methods, a protective surface layer is formed upon at least one electrode in the electrolytic reduction cell and, optionally, upon the lining of the cell. This protective surface layer comprises a material that, at the operating conditions of the cell: (a) is not substantially reduced by the metal product; (b) is not substantially reactive with the cell electrolyte to form materials that are reactive with the metal product; and, (c) has an electrochemical potential that is more electronegative than that of the compound undergoing electrolysis to produce the metal product of the cell. The protective surface layer can be formed upon an electrode metal layer comprising a material, the oxide of which also satisfies the protective layer selection criteria. The protective layer material can also be used on the surface of a cell lining.

Development of Pinhole-Free Amorphous Aluminum Oxide Protective Layers for Biomedical Device Applications

PubMed Central

Litvinov, Julia; Wang, Yi-Ju; George, Jinnie; Chinwangso, Pawilai; Brankovic, Stanko; Willson, Richard C.; Litvinov, Dmitri

2013-01-01

This paper describes synthesis of ultrathin pinhole-free insulating aluminum oxide layers for electronic device protection in corrosive liquid environments, such as phosphate buffered saline (PBS) or clinical fluids, to enable emerging biomedical applications such as biomolecular sensors. A pinhole-free 25-nm thick amorphous aluminum oxide layer has been achieved using ultra-high vacuum DC magnetron reactive sputtering of aluminum in oxygen/argon plasma followed by oxygen plasma post-processing. Deposition parameters were optimized to achieve the best corrosion protection of lithographically defined device structures. Electrochemical deposition of copper through the aluminum oxide layers was used to detect the presence (or absence) of pinholes. FTIR, XPS, and spectroscopic ellipsometry were used to characterize the material properties of the protective layers. Electrical resistance of the copper device structures protected by the aluminum oxide layers and exposed to a PBS solution was used as a metric to evaluate the long-term stability of these device structures. PMID:23682201

Continuous fiber reinforced mesh bond coat for environmental barrier coating system

DOEpatents

Zhang, James; Das, Rupak; Roberts III, Herbert Chidsey; Delvaux, John McConnell

2017-09-26

A gas turbine blade may have a bond coat applied to its surface. A porous substrate may be applied to the bond layer and one or more protective layers may be applied to the bond layer such that the fiber mesh is embedded between the bond layer and the protective layer to prevent creep.

Heat Pipes for Alaskan Oil

NASA Technical Reports Server (NTRS)

1976-01-01

McDonnel Douglas Corporation is using a heat-pipe device, developed through the space program, to transport oil from Alaska's rich North Slope fields. It is being used to keep the ground frozen along the 798- mile pipeline saving hundreds of millions of dollars and protecting the tundra environment. Heatpipes are totally automatic, they sense and respond to climatic conditions with no moving parts, require no external power, and never need adjustment or servicing.

Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA.

PubMed

Washetine, Kevin; Kara-Borni, Mehdi; Heeke, Simon; Bonnetaud, Christelle; Félix, Jean-Marc; Ribeyre, Lydia; Bence, Coraline; Ilié, Marius; Bordone, Olivier; Pedro, Marine; Maitre, Priscilla; Tanga, Virginie; Gormally, Emmanuelle; Mossuz, Pascal; Lorimier, Philippe; Marquette, Charles Hugo; Mouroux, Jérôme; Cohen, Charlotte; Lassalle, Sandra; Long-Mira, Elodie; Clément, Bruno; Dagher, Georges; Hofman, Véronique; Hofman, Paul

2018-06-11

Collected specimens for research purposes may or may not be made available depending on their scarcity and/or on the project needs. Their protection against degradation or in the event of an incident is pivotal. Duplication and storage on a different site is the best way to assure their sustainability. The conservation of samples at room temperature (RT) by duplication can facilitate their protection. We describe a security system for the collection of non-small cell lung cancers (NSCLC) stored in the biobank of the Nice Hospital Center, France, by duplication and conservation of lyophilized (dried), encapsulated DNA kept at RT. Therefore, three frozen tissue collections from non-smoking, early stage and sarcomatoid carcinoma NSCLC patients were selected for this study. DNA was extracted, lyophilized and encapsulated at RT under anoxic conditions using the DNAshell technology. In total, 1974 samples from 987 patients were encapsulated. Six and two capsules from each sample were stored in the biobanks of the Nice and Grenoble (France) Hospitals, respectively. In conclusion, DNA maintained at RT allows for the conservation, duplication and durability of collections of interest stored in biobanks. This is a low-cost and safe technology that requires a limited amount of space and has a low environmental impact.

Photovoltaic module and laminate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bunea, Gabriela E.; Kim, Sung Dug; Kavulak, David F.J.

A photovoltaic module is disclosed. The photovoltaic module has a first side directed toward the sun during normal operation and a second, lower side. The photovoltaic module comprises a perimeter frame and a photovoltaic laminate at least partially enclosed by and supported by the perimeter frame. The photovoltaic laminate comprises a transparent cover layer positioned toward the first side of the photovoltaic module, an upper encapsulant layer beneath and adhering to the cover layer, a plurality of photovoltaic solar cells beneath the upper encapsulant layer, the photovoltaic solar cells electrically interconnected, a lower encapsulant layer beneath the plurality of photovoltaicmore » solar cells, the upper and lower encapsulant layers enclosing the plurality of photovoltaic solar cells, and a homogenous rear environmental protection layer, the rear environmental protection layer adhering to the lower encapsulant layer, the rear environmental protection layer exposed to the ambient environment on the second side of the photovoltaic module.« less

Interspecies somatic cell nuclear transfer in Asiatic cheetah using nuclei derived from post-mortem frozen tissue in absence of cryo-protectant and in vitro matured domestic cat oocytes.

PubMed

Moulavi, F; Hosseini, S M; Tanhaie-Vash, N; Ostadhosseini, S; Hosseini, S H; Hajinasrollah, M; Asghari, M H; Gourabi, H; Shahverdi, A; Vosough, A D; Nasr-Esfahani, M H

2017-03-01

Recent accomplishments in the field of somatic cell nuclear transfer (SCNT) hold tremendous promise to prevent rapid loss of animal genetic resources using ex situ conservation technology. Most of SCNT studies use viable cells for nuclear transfer into recipient oocytes. However, preparation of live cells in extreme circumstances, in which post-mortem material of endangered/rare animals is improperly retained frozen, is difficult, if not impossible. This study investigated the possibility of interspecies-SCNT (iSCNT) in Asiatic cheetah (Acinonyx jubatus venaticus), a critically endangered subspecies, using nuclei derived from frozen tissue in absence of cryo-protectant at -20 °C and in vitro matured domestic cat oocytes. No cells growth was detected in primary culture of skin and tendon pieces or following culture of singled cells prepared by enzymatic digestion. Furthermore, no live cells were detected following differential viable staining and almost all cells had ruptured membrane. Therefore, direct injection of donor nuclei into enucleated cat oocytes matured in vitro was carried out for SCNT experiments. Early signs of nuclear remodeling were observed as early as 2 h post-iSCNT and significantly increased at 4 h post-iSCNT. The percentages of iSCNT reconstructs that cleaved and developed to 4-16 cell and morula stages were 32.3 ± 7.3, 18.2 ± 9.8 and 5.9 ± 4.3%, respectively. However, none of the iSCNT reconstructs developed to the blastocyst stage. When domestic cat somatic and oocytes were used for control SCNT and parthenogenetic activation, the respective percentages of oocytes that cleaved (51.3 ± 13.9 and 77.3 ± 4.0%) and further developed to the blastocyst stage (11.3 ± 3.3 and 16.8 ± 3.8%) were comparable. In summary, this study demonstrated that enucleated cat oocytes can partially remodel and reactivate non-viable nuclei of Asiatic cheetah and support its reprogramming back to the embryonic stage. To our knowledge, this is the first report of iSCNT in cheetah using non-viable frozen cells. Copyright © 2016 Elsevier Inc. All rights reserved.

Permittivity estimation over Mars by using SHARAD data: the Cerberus Palus area

NASA Astrophysics Data System (ADS)

Alberti, Giovanni; Castaldo, Luigi; Orosei, Roberto; Frigeri, Alessandro; Cirillo, Giuseppe

2012-09-01

Cerberus Palus is a thoroughly studied region of Mars, characterized by evident platy textures that were interpreted either as evidence for a frozen sea close to Mars' equator or as being resultant of lava, mud or ice-flows coming from Cerberus Fossae through Athabasca Valles. Radargrams provided by radar sounder SHARAD clearly show the presence of subsurface layers in the area. By exploiting the great amount of available data, authors have performed an accurate quantitative analysis aimed to estimate electromagnetic properties of surface and subsurface layers, in terms of permittivity and attenuation. To this aim, a simplified electromagnetic approach has been used, but taking into account effects of scattering due to surface roughness, for avoiding overestimated results. This has been done by using theory of electromagnetic scattering from fractal surfaces and by estimating needed parameters from topographic data provided by MOLA. Three distinct geologic formations have been analyzed, namely a part of Zephyria Planum, the Cerberus plains and the bedrock beneath the plains. The retrieved electromagnetic parameters have been also modeled as a mixture of volcanic rocks with either ice or air. The Zephyria Planum material was found to be significantly porous [50-60%] with an attenuation more likely compatible with empty pores. Ambiguous results were obtained for the plains material, being the resulting porosity high in both the cases of empty [40-50%] and of ice-filled [80%] pores. The obtained results do not allow for evidence of a frozen sea on Cerberus Palus area.

Stable water isotopic composition of the Antarctic subglacial Lake Vostok: implications for understanding the lake's hydrology.

PubMed

Ekaykin, Alexey A; Lipenkov, Vladimir Y; Kozachek, Anna V; Vladimirova, Diana O

2016-01-01

We estimated the stable isotopic composition of water from the subglacial Lake Vostok using two different sets of samples: (1) water frozen on the drill bit immediately after the first lake unsealing and (2) water frozen in the borehole after the unsealing and re-drilled one year later. The most reliable values of the water isotopic composition are: -59.0 ± 0.3 ‰ for oxygen-18, -455 ± 1 ‰ for deuterium and 17 ± 1 ‰ for d-excess. This result is also confirmed by the modelling of isotopic transformations in the water which froze in the borehole, and by a laboratory experiment simulating this process. A comparison of the newly obtained water isotopic composition with that of the lake ice (-56.2 ‰ for oxygen-18, -442.4 ‰ for deuterium and 7.2 ‰ for d-excess) leads to the conclusion that the lake ice is very likely formed in isotopic equilibrium with water. In turn, this means that ice is formed by a slow freezing without formation of frazil ice crystals and/or water pockets. This conclusion agrees well with the observed physical and chemical properties of the lake's accreted ice. However, our estimate of the water's isotopic composition is only valid for the upper water layer and may not be representative for the deeper layers of the lake, so further investigations are required.

Raman scattering evidence of hydrohalite formation on frozen yeast cells.

PubMed

Okotrub, K A; Surovtsev, N V

2013-02-01

We studied yeast cells in physiological solution during freezing by Raman microspectroscopy technique. The purpose was to find out the origin of a sharp peak near ∼3430cm(-1) in Raman spectrum of frozen mammalian cells, observed earlier (J. Dong et al., Biophys. J. 99 (2010) 2453), which presumably could be used as an indicator of intracellar ice appearance. We have shown that this line (actually doublet of 3408 and 3425cm(-1)) corresponds to Raman spectrum of hydrohalite (NaCl⋅2H(2)O), which is formed as the result of the eutectic crystallization of the liquid solution around the cells. We also show that the spatial distribution of hydrohalite in the sample significantly depends on the cooling rate. At lower cooling rate (1°C/min), products of eutectic crystallization form layer on the cell surface which thickness varies for different cells and can reach ∼1μm in thickness. At higher cooling rate (20°C/min), the hydrohalite distribution appears more homogeneous, in the sample, and the eutectic crystallization layer around the cells was estimated to be less than ∼20nm. These experimental results are consistent with scenarios predicted by the two-factor hypothesis for freezing induced cell injury. This work demonstrates a potential of Raman microspectroscopy to study peculiarities of the eutectic crystallization around single cells in vivo with the high spatial resolution. Copyright © 2012 Elsevier Inc. All rights reserved.

The distribution of serum albumin in the rat testis, studied by electron microscope immunocytochemistry on ultrathin frozen sections.

PubMed

Christensen, A K; Komorowski, T E; Wilson, B; Ma, S F; Stevens, R W

1985-05-01

The distribution of serum albumin is of interest in the rat testis because this protein is the principal carrier for testosterone in the plasma and interstitial fluid of this species. We have localized extravascular serum albumin in the rat testis at the electron microscope level, using gold particle immunocytochemistry on ultrathin frozen sections of tissue fixed lightly by perfusion. The same localization was obtained with three different antisera. Preabsorption and normal rabbit serum controls were negative, and Western blots of testis extracts showed major activity only at the molecular weight of albumin. Serum albumin occurred in substantial concentration throughout extracellular space in the interstitial tissue, as well as in the space between the boundary layer and the base of the seminiferous epithelium. Immunoreactivity extended between Sertoli cells, as well as around spermatogonia and early primary spermatocytes (to stage 11), but did not traverse the Sertoli-Sertoli junctions that comprise the blood-testis barrier. Macrophages in the interstitial tissue showed some endocytic activity. If perfusion fixation was carried out in a manner that flushed most of the albumin from the interstitial space, then a layer of albumin remained on the surface of Leydig cells and many macrophages but was minimal or absent on the surface of other cell types that are normally in contact with albumin, such as Sertoli cells, spermatogonia, myoid cells, lymphatic endothelium, fibroblasts, or cells of blood vessels.

Acquisition of thin coronal sectional dataset of cadaveric liver.

PubMed

Lou, Li; Liu, Shu Wei; Zhao, Zhen Mei; Tang, Yu Chun; Lin, Xiang Tao

2014-04-01

To obtain the thin coronal sectional anatomic dataset of the liver by using digital freezing milling technique. The upper abdomen of one Chinese adult cadaver was selected as the specimen. After CT and MRI examinations verification of absent liver lesions, the specimen was embedded with gelatin in stand erect position and frozen under profound hypothermia, and the specimen was then serially sectioned from anterior to posterior layer by layer with digital milling machine in the freezing chamber. The sequential images were captured by means of a digital camera and the dataset was imported to imaging workstation. The thin serial section of the liver added up to 699 layers with each layer being 0.2 mm in thickness. The shape, location, structure, intrahepatic vessels and adjacent structures of the liver was displayed clearly on each layer of the coronal sectional slice. CT and MR images through the body were obtained at 1.0 and 3.0 mm intervals, respectively. The methodology reported here is an adaptation of the milling methods previously described, which is a new data acquisition method for sectional anatomy. The thin coronal sectional anatomic dataset of the liver obtained by this technique is of high precision and good quality.

21 CFR 160.110 - Frozen eggs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen eggs. 160.110 Section 160.110 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.110 Frozen eggs. (a) Frozen eggs, frozen whole eggs, frozen mixed eggs is the food prepared by freezing...

21 CFR 160.110 - Frozen eggs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen eggs. 160.110 Section 160.110 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.110 Frozen eggs. (a) Frozen eggs, frozen whole eggs, frozen mixed eggs is the food prepared by freezing...

Magnetic Reconnection and Modification of the Hall Physics Due to Cold Ions at the Magnetopause

NASA Technical Reports Server (NTRS)

Andre, M.; Li, W.; Toldeo-Redondo, S.; Khotyaintsev, Yu. V.; Vaivads, A.; Graham, D. B.; Norgren, C.; Burch, J.; Lindqvist, P.-A.; Marklund, G.;

Fourier transform wavefront control with adaptive prediction of the atmosphere.

PubMed

Poyneer, Lisa A; Macintosh, Bruce A; Véran, Jean-Pierre

2007-09-01

Predictive Fourier control is a temporal power spectral density-based adaptive method for adaptive optics that predicts the atmosphere under the assumption of frozen flow. The predictive controller is based on Kalman filtering and a Fourier decomposition of atmospheric turbulence using the Fourier transform reconstructor. It provides a stable way to compensate for arbitrary numbers of atmospheric layers. For each Fourier mode, efficient and accurate algorithms estimate the necessary atmospheric parameters from closed-loop telemetry and determine the predictive filter, adjusting as conditions change. This prediction improves atmospheric rejection, leading to significant improvements in system performance. For a 48x48 actuator system operating at 2 kHz, five-layer prediction for all modes is achievable in under 2x10(9) floating-point operations/s.

Recent progress in the study of protective rust-layer formation on weathering steel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamashita, M.; Misawa, T.

Latest understanding of protective rust layer on weathering steel and its application for structural steels is discussed. Phase transformation of the weathering steel rust layer during long-time exposure brings {alpha}-(Fe{sub 1{minus}x},Cr{sub x})OOH, Cr-substituted goethite, as the final protective rust layer. It is said that the Cr content in the Cr-substituted goethite layer increases gradiently with reaching the rust-steel interface. This increase in the Cr content gives densely packed fine crystal structure end cation selective ability, which impedes the penetration of aggressive corrosives including anions such as Cl{sup {minus}} and SO{sub 4}{sup 2{minus}}. Quite recently, new surface-treatment technique employing Cr{sub 2}(SO{submore » 4}){sub 3}, was proposed, which provides a possibility for obtaining the protective rust layer in a relatively short period even in the severe environment such as coastal region.« less

Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA.

PubMed

Wang, Hongyang; Owens, James D; Shih, Joanna H; Li, Ming-Chung; Bonner, Robert F; Mushinski, J Frederic

2006-04-27

Gene expression profiling by microarray analysis of cells enriched by laser capture microdissection (LCM) faces several technical challenges. Frozen sections yield higher quality RNA than paraffin-imbedded sections, but even with frozen sections, the staining methods used for histological identification of cells of interest could still damage the mRNA in the cells. To study the contribution of staining methods to degradation of results from gene expression profiling of LCM samples, we subjected pellets of the mouse plasma cell tumor cell line TEPC 1165 to direct RNA extraction and to parallel frozen sectioning for LCM and subsequent RNA extraction. We used microarray hybridization analysis to compare gene expression profiles of RNA from cell pellets with gene expression profiles of RNA from frozen sections that had been stained with hematoxylin and eosin (H&E), Nissl Stain (NS), and for immunofluorescence (IF) as well as with the plasma cell-revealing methyl green pyronin (MGP) stain. All RNAs were amplified with two rounds of T7-based in vitro transcription and analyzed by two-color expression analysis on 10-K cDNA microarrays. The MGP-stained samples showed the least introduction of mRNA loss, followed by H&E and immunofluorescence. Nissl staining was significantly more detrimental to gene expression profiles, presumably owing to an aqueous step in which RNA may have been damaged by endogenous or exogenous RNAases. RNA damage can occur during the staining steps preparatory to laser capture microdissection, with the consequence of loss of representation of certain genes in microarray hybridization analysis. Inclusion of RNAase inhibitor in aqueous staining solutions appears to be important in protecting RNA from loss of gene transcripts.

Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA

PubMed Central

Wang, Hongyang; Owens, James D; Shih, Joanna H; Li, Ming-Chung; Bonner, Robert F; Mushinski, J Frederic

2006-01-01

Background Gene expression profiling by microarray analysis of cells enriched by laser capture microdissection (LCM) faces several technical challenges. Frozen sections yield higher quality RNA than paraffin-imbedded sections, but even with frozen sections, the staining methods used for histological identification of cells of interest could still damage the mRNA in the cells. To study the contribution of staining methods to degradation of results from gene expression profiling of LCM samples, we subjected pellets of the mouse plasma cell tumor cell line TEPC 1165 to direct RNA extraction and to parallel frozen sectioning for LCM and subsequent RNA extraction. We used microarray hybridization analysis to compare gene expression profiles of RNA from cell pellets with gene expression profiles of RNA from frozen sections that had been stained with hematoxylin and eosin (H&E), Nissl Stain (NS), and for immunofluorescence (IF) as well as with the plasma cell-revealing methyl green pyronin (MGP) stain. All RNAs were amplified with two rounds of T7-based in vitro transcription and analyzed by two-color expression analysis on 10-K cDNA microarrays. Results The MGP-stained samples showed the least introduction of mRNA loss, followed by H&E and immunofluorescence. Nissl staining was significantly more detrimental to gene expression profiles, presumably owing to an aqueous step in which RNA may have been damaged by endogenous or exogenous RNAases. Conclusion RNA damage can occur during the staining steps preparatory to laser capture microdissection, with the consequence of loss of representation of certain genes in microarray hybridization analysis. Inclusion of RNAase inhibitor in aqueous staining solutions appears to be important in protecting RNA from loss of gene transcripts. PMID:16643667

Coating with overlay metallic-cermet alloy systems

NASA Technical Reports Server (NTRS)

Gedwill, M. A.; Levine, S. R.; Glasgow, T. K. (Inventor)

1984-01-01

A base layer of an oxide dispersed, metallic alloy (cermet) is arc plasma sprayed onto a substrate, such as a turbine blade, vane, or the like, which is subjected to high temperature use. A top layer of an oxidation, hot corrosion, erosion resistant alloy of nickel, cobalt, or iron is then arc plasma sprayed onto the base layer. A heat treatment is used to improve the bonding. The base layer serves as an inhibitor to interdiffusion between the protective top layer and the substrate. Otherwise, the 10 protective top layer would rapidly interact detrimentally with the substrate and degrade by spalling of the protective oxides formed on the outer surface at elevated temperatures.

Terminologie alimentaire (Food Terminology).

ERIC Educational Resources Information Center

Pelletier, Jean-Francois

1980-01-01

Translations and descriptions are given in French for a number of English food terms: convenience foods, fast foods, fast foods industry, fast foods restaurant, frozen foods, deep frozen foods, fast frozen foods, quick frozen foods, dry frozen foods. (MSE)

Frozen shoulder and the Big Five personality traits.

PubMed

Debeer, Philippe; Franssens, Fien; Roosen, Isabelle; Dankaerts, Wim; Claes, Laurence

2014-02-01

In the past, several studies have suggested the existence of a "periarthritic personality" in patients with frozen shoulder. We conducted a study to determine differences in personality traits in patients with primary and secondary frozen shoulders. We prospectively evaluated 118 patients (84 women and 34 men; mean age, 53.8 years; SD 7.56) with a frozen shoulder. Of these patients, 48 had an idiopathic frozen shoulder and 70 had a secondary frozen shoulder. Personality traits were determined by the NEO Five-Factor Inventory (NEO-FFI) scale. This questionnaire measures the 5 major personality traits and is based on the norms determined in a neutral test situation for 2415 controls. Compared with healthy controls, no differences in personality traits were found in patients with primary and secondary frozen shoulder, except for Conscientiousness and Extraversion, for which patients with secondary frozen shoulder scored significantly higher than healthy controls. Patients with primary frozen shoulder scored significantly higher on Openness to Experience than did patients with secondary frozen shoulder; on the other 4 Big Five personality traits, no significant differences were found between patients with primary and secondary frozen shoulder. More specifically, patients with idiopathic frozen shoulder did not score higher on the trait Neuroticism as would be expected from previous publications. Our study results do not indicate that patients with an idiopathic frozen shoulder have a specific personality compared with healthy controls. Only a few differences were found in personality traits when the entire frozen shoulder group was compared with healthy controls and between patients with primary and secondary frozen shoulders. The results of this study suggest that these differences are not sufficient to speak about a specific "frozen shoulder personality." Copyright © 2014 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Mosby, Inc. All rights reserved.

Effect of Surface Preparation on Residual Stresses in Multilayer Coatings and the Consequences for Disbondment Following Construction Damage and Exposure to In-Service Stress

DOT National Transportation Integrated Search

2009-01-01

Underground pipelines are protected by a combination of cathodic protection and a protective coating. Multi-layer coatings offer protection against corrosion and from mechanical damage during construction or during service. Multi-layer coatings are w...

75 FR 32306 - Perishable Agricultural Commodities Act: Impact of Post-Default Agreements on Trust Protection...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-08

...); American Banana Co. v. Republic Nat. Bank of N.Y., 362 F.3d 33 (2nd Cir. 2004); Patterson Frozen Foods, Inc. v. Crown Foods, Int'l, 307 F.3d 666, 667 (7th Cir. 2002); Greg Orchards Produce, Inc. v. P. Roncone J., 180 F.3d 888, 892 (7th Cir. 1999); Idahoan Fresh v. Advantage Produce, Inc., 157 F.3d 197, 205...

The Eccentric Behavior of Nearly Frozen Orbits

NASA Technical Reports Server (NTRS)

Sweetser, Theodore H.; Vincent, Mark A.

2013-01-01

Frozen orbits are orbits which have only short-period changes in their mean eccentricity and argument of periapse, so that they basically keep a fixed orientation within their plane of motion. Nearly frozen orbits are those whose eccentricity and argument of periapse have values close to those of a frozen orbit. We call them "nearly" frozen because their eccentricity vector (a vector whose polar coordinates are eccentricity and argument of periapse) will stay within a bounded distance from the frozen orbit eccentricity vector, circulating around it over time. For highly inclined orbits around the Earth, this distance is effectively constant over time. Furthermore, frozen orbit eccentricity values are low enough that these orbits are essentially eccentric (i.e., off center) circles, so that nearly frozen orbits around Earth are bounded above and below by frozen orbits.

Nozzle Flow with Vibrational Nonequilibrium. Ph.D. Thesis

NASA Technical Reports Server (NTRS)

Landry, John Gary

1995-01-01

Flow of nitrogen gas through a converging-diverging nozzle is simulated. The flow is modeled using the Navier-Stokes equations that have been modified for vibrational nonequilibrium. The energy equation is replaced by two equations. One equation accounts for energy effects due to the translational and rotational degrees of freedom, and the other accounts for the affects due to the vibrational degree of freedom. The energy equations are coupled by a relaxation time which measures the time required for the vibrational energy component to equilibrate with the translational and rotational energy components. An improved relaxation time is used in this thesis. The equations are solved numerically using the Steger-Warming flux vector splitting method and the Implicit MacCormack method. The results show that uniform flow is produced outside of the boundary layer. Nonequilibrium exists in both the converging and diverging nozzle sections. The boundary layer region is characterized by a marked increase in translational-rotational temperature. The vibrational temperature remains frozen downstream of the nozzle, except in the boundary layer.

Greenland Ice Sheet in 3D Cutaway

NASA Image and Video Library

2017-12-08

Peering into the thousands of frozen layers inside Greenland’s ice sheet is like looking back in time. Each layer provides a record of what Earth’s climate was like at the dawn of civilization, or during the last ice age, or during an ancient period of warmth similar to the one we experience today. Scientists using ice-penetrating radar data collected by NASA’s Operation IceBridge and earlier airborne campaigns have built the first-ever comprehensive map of layers deep inside the Greenland Ice Sheet. View the full video: youtu.be/u0VbPE0TOtQ Credit: NASA’s Goddard Space Flight Center NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

On spontaneous formation of current sheets: Untwisted magnetic fields

NASA Astrophysics Data System (ADS)

Bhattacharyya, R.; Low, B. C.; Smolarkiewicz, P. K.

2010-11-01

This is a study of the spontaneous formation of electric current sheets in an incompressible viscous fluid with perfect electrical conductivity, governed by the magnetohydrodynamic Navier-Stokes equations. Numerical solutions to two initial value problems are presented for a three-dimensional, periodic, untwisted magnetic field evolving, with no change in magnetic topology under the frozen-in condition and at characteristic fluid Reynolds numbers of the order of 500, from a nonequilibrium initial state with the fluid at rest. The evolution converts magnetic free energy into kinetic energy to be all dissipated away by viscosity so that the field settles into a minimum-energy, static equilibrium. The solutions demonstrate that, as a consequence of the frozen-in condition, current sheets must form during the evolution despite the geometric simplicity of the prescribed initial fields. In addition to the current sheets associated with magnetic neutral points and field reversal layers, other sheets not associated with such magnetic features are also in evidence. These current sheets form on magnetic flux surfaces. This property is used to achieve a high degree of the frozen-in condition in the simulations, by describing the magnetic field entirely in terms of the advection of its flux surfaces and integrating the resulting governing equations with a customized version of a general-purpose high-resolution (viz., nonoscillatory) hydrodynamical simulation code EULAG [J. M. Prusa et al., Comput. Fluids 37, 1193 (2008)]. Incompressibility imposes the additional global constraint that the flux surfaces must evolve with no change in the spatial volumes they enclose. In this approach, current sheet formation is demonstrated graphically by the progressive pressing together of suitably selected flux surfaces until their separation has diminished below the minimal resolved distance on a fixed grid. The frozen-in condition then fails in the simulation as the field reconnects through an effecting numerical resistivity. The principal results are related to the Parker theory of current-sheet formation and dissipation in the solar corona.

Martian 'Swiss Cheese'

NASA Technical Reports Server (NTRS)

2000-01-01

This image is illuminated by sunlight from the upper left.

Looking like pieces of sliced and broken swiss cheese, the upper layer of the martian south polar residual cap has been eroded, leaving flat-topped mesas into which are set circular depressions such as those shown here. The circular features are depressions, not hills. The largest mesas here stand about 4 meters (13 feet) high and may be composed of frozen carbon dioxide and/or water. Nothing like this has ever been seen anywhere on Mars except within the south polar cap, leading to some speculation that these landforms may have something to do with the carbon dioxide thought to be frozen in the south polar region. On Earth, we know frozen carbon dioxide as 'dry ice'. On Mars, as this picture might be suggesting, there may be entire landforms larger than a small town and taller than 2 to 3 men and women that consist, in part, of dry ice.

No one knows for certain whether frozen carbon dioxide has played a role in the creation of the 'swiss cheese' and other bizarre landforms seen in this picture. The picture covers an area 3 x 9 kilometers (1.9 x 5.6 miles) near 85.6oS, 74.4oW at a resolution of 7.3 meters (24 feet) per pixel. This picture was taken by the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) during early southern spring on August 3, 1999.

Malin Space Science Systems and the California Institute of Technology built the MOC using spare hardware from the Mars Observer mission. MSSS operates the camera from its facilities in San Diego, CA. The Jet Propulsion Laboratory's Mars Surveyor Operations Project operates the Mars Global Surveyor spacecraft with its industrial partner, Lockheed Martin Astronautics, from facilities in Pasadena, CA and Denver, CO.

21 CFR 102.26 - Frozen “heat and serve” dinners.

Code of Federal Regulations, 2012 CFR

2012-04-01

... Nonstandardized Foods § 102.26 Frozen “heat and serve” dinners. (a) A frozen “heat and serve” dinner: (1) Shall... consists of all of the following: (1) The phrase “frozen ‘heat and serve’ dinner,” except that the name of... precede the word “dinner” (e.g., “frozen chicken dinner” or “frozen heat and serve beef dinner”). The...

21 CFR 102.26 - Frozen “heat and serve” dinners.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Nonstandardized Foods § 102.26 Frozen “heat and serve” dinners. (a) A frozen “heat and serve” dinner: (1) Shall... consists of all of the following: (1) The phrase “frozen ‘heat and serve’ dinner,” except that the name of... precede the word “dinner” (e.g., “frozen chicken dinner” or “frozen heat and serve beef dinner”). The...

21 CFR 102.26 - Frozen “heat and serve” dinners.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Nonstandardized Foods § 102.26 Frozen “heat and serve” dinners. (a) A frozen “heat and serve” dinner: (1) Shall... consists of all of the following: (1) The phrase “frozen ‘heat and serve’ dinner,” except that the name of... precede the word “dinner” (e.g., “frozen chicken dinner” or “frozen heat and serve beef dinner”). The...

The Development of Alternative Food Cost Indexes

DTIC Science & Technology

1974-11-01

Frozen frufts, vegetables , fruit juices , and cake mixes are added and the quantity of purchased bread increased in this FCI. Comment was made that some... Grain , Frozen Vegetables , Mixed, Frozen Beans, Green, Frozen Peaches, Canned Apples, Fresh Oranges, Fresh Strawberries, Frozen Juice , Pineapple...also falls to recognize such accepted food service practices as the use of frozen fruits , vegetables , and juices , or prepared cake mixes, Tj,e

Operational Guides for Frozen Products Prepared by the F. E. Warren Foil Pack Facility

DTIC Science & Technology

1985-07-01

steaks and shake off excess. c. Place dredged steaks on cookie sheets in one layer with no more than one quarter steak overlap. Place about 25-26 steaks...not be more than 11 oz. c. Place foils on cookie sheets and bake in oven until topping if golden brown. d. Cover, lable, and freeze. 47...attachment on mixer. b. Add eggs and vanilla and mix well. c. Add flour, salt, and cinnamon; mix until dough is well blended. d. Spread 5 ib of dough on

Cryo-transmission electron microscopy of frozen-hydrated sections of Escherichia coli and Pseudomonas aeruginosa.

PubMed

Matias, Valério R F; Al-Amoudi, Ashraf; Dubochet, Jacques; Beveridge, Terry J

2003-10-01

High-pressure freezing of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1 in the presence of cryoprotectants provided consistent vitrification of cells so that frozen-hydrated sections could be cut, providing approximately 2-nm resolution of structure. The size and shape of the bacteria, as well as their surface and cytoplasmic constituents, were nicely preserved and compared well with other published high-resolution techniques. Cells possessed a rich cytoplasm containing a diffuse dispersion of ribosomes and genetic material. Close examination of cells revealed that the periplasmic space was compressed during cryosectioning, a finding which provided supporting evidence that this space is filled by a compressible gel. Since the outer membrane and peptidoglycan layer are bonded together via lipoproteins, the space between them (although still part of the periplasmic space) was not as compacted. Even when this cryosectioning compression was taken into account, there was still substantial variability in the width of the periplasmic space. It is possible that the protoplast has some capacity to float freely within the periplasm.

Cold-stage microscopy system for fast-frozen liquids.

PubMed

Talmon, Y; Davis, H T; Scriven, L E; Thomas, E L

1979-06-01

The least artifact-laden fixation technique for examining colloidal suspensions, microemulsions, and other microstructured liquids in the electron microscope appears to be thermal fixation, i.e., ultrafast freezing of the liquid specimen. For rapid-enough cooling and for observation in TEM/STEM a thin sample is needed. The need is met by trapping a thin layer ( approximately 100 nm) of liquid between two polyimide films ( approximately 40 nm thickness) mounted on copper grids and immersing the resulting sandwich in liquid nitrogen at its melting point. For liquids containing water, polyimides films are used since this polymer is far less susceptible to the electron beam damage observed for the commonly used polymer films such as Formvar and collodion in contact with ice. Transfer of the frozen sample into the microscope column without deleterious frost deposition and warming is accomplished with a new transfer module for the cooling stage of the JEOL JEM-100CX microscope, which makes a true cold stage out of a device originally intended for cooling specimens inside the column. Sample results obtained with the new fast-freeze, cold-stage microscopy system are given.

Effects of various cryoprotectants on the quality of frozen-thawed immature bovine (Qinchuan cattle) calf testicular tissue.

PubMed

Zhang, X-G; Li, H; Hu, J-H

2017-11-01

To investigate the effects of different concentrations of various cryoprotectants (CPs) on the cell viability as well as expression of spermatogenesis-related genes, such as CREM, Stra8 and HSP70-2 in frozen-thawed bovine calf testicular tissue, immature bovine (Qinchuan cattle) calf testicular tissue was collected and cryopreserved in the cryomedia containing different concentrations (5%, 10%, 15% and 20%) of the following three CPs: glycerol, ethylene glycol (EG) and dimethyl sulphoxide (DMSO) respectively. After 1 month cryopreservation in liquid nitrogen, cell viability was evaluated using Trypan blue exclusion under a bright-field microscope. The mRNA expression of the three genes was also evaluated using qRT-PCR. The results indicated that different concentrations of glycerol, EG and DMSO in cryomedia during cryopreservation could protect bovine calf testicular tissue in various ways to avoid freezing or cryopreservation-induced expression changes in spermatogenesis-related genes. The highest cell viability and the three spermatogenesis-related genes (CREM, Stra8 and HSP70-2) expression level came from the cryomedia containing glycerol, EG and DMSO at 10% concentration respectively (p < .05). Meanwhile, compared with the other CPs, the frozen-thawed bovine calf testicular tissue treated with 10% DMSO exhibited the highest cell viability and mRNA expression level of the spermatogenesis-related genes (CREM, Stra8 and HSP70-2). © 2017 Blackwell Verlag GmbH.

Reduced glutathione and procaine hydrochloride protect the nucleoprotein structure of boar spermatozoa during freeze-thawing by stabilising disulfide bonds.

PubMed

Yeste, Marc; Flores, Eva; Estrada, Efrén; Bonet, Sergi; Rigau, Teresa; Rodríguez-Gil, Joan E

2013-01-01

One important change the head of boar spermatozoa during freeze-thawing is the destabilisation of its nucleoprotein structure due to a disruption of disulfide bonds. With the aim of better understanding these changes in frozen-thawed spermatozoa, two agents, namely reduced glutathione (GSH) and procaine hydrochloride (ProHCl), were added at different concentrations to the freezing media at different concentrations and combinations over the range 1-2mM. Then, 30 and 240 min after thawing, cysteine-free residue levels of boar sperm nucleoproteins, DNA fragmentation and other sperm functional parameters were evaluated. Both GSH and ProHCl, at final concentrations of 2mM, induced a significant (P<0.05) increase in the number of non-disrupted sperm head disulfide bonds 30 and 240 min after thawing compared with the frozen-thawed control. This effect was accompanied by a significant (P<0.05) decrease in DNA fragmentation 240 min after thawing. Concomitantly, 1 and 2mM GSH, but not ProHCl at any of the concentrations tested, partially counteracted the detrimental effects caused by freeze-thawing on sperm peroxide levels, motility patterns and plasma membrane integrity. In conclusion, the results show that both GSH and ProHCl have a stabilising effect on the nucleoprotein structure of frozen-thawed spermatozoa, although only GSH exerts an appreciable effect on sperm viability.

Interfacial band-edge engineered TiO2 protection layer on Cu2O photocathodes for efficient water reduction reaction

NASA Astrophysics Data System (ADS)

Choi, Jaesuk; Song, Jun Tae; Jang, Ho Seong; Choi, Min-Jae; Sim, Dong Min; Yim, Soonmin; Lim, Hunhee; Jung, Yeon Sik; Oh, Jihun

2017-01-01

Photoelectrochemical (PEC) water splitting has emerged as a potential pathway to produce sustainable and renewable chemical fuels. Here, we present a highly active Cu2O/TiO2 photocathode for H2 production by enhancing the interfacial band-edge energetics of the TiO2 layer, which is realized by controlling the fixed charge density of the TiO2 protection layer. The band-edge engineered Cu2O/TiO2 (where TiO2 was grown at 80 °C via atomic layer deposition) enhances the photocurrent density up to -2.04 mA/cm2 at 0 V vs. RHE under 1 sun illumination, corresponding to about a 1,200% enhancement compared to the photocurrent density of the photocathode protected with TiO2 grown at 150 °C. Moreover, band-edge engineering of the TiO2 protection layer prevents electron accumulation at the TiO2 layer and enhances both the Faraday efficiency and the stability for hydrogen production during the PEC water reduction reaction. This facile control over the TiO2/electrolyte interface will also provide new insight for designing highly efficient and stable protection layers for various other photoelectrodes such as Si, InP, and GaAs. [Figure not available: see fulltext.

Evaluation of atomic layer deposited alumina as a protective layer for domestic silver articles: Anti-corrosion test in artificial sweat

NASA Astrophysics Data System (ADS)

Park, Suk Won; Han, Gwon Deok; Choi, Hyung Jong; Prinz, Fritz B.; Shim, Joon Hyung

2018-05-01

This study evaluated the effectiveness of alumina fabricated by atomic layer deposition (ALD) as a protective coating for silver articles against the corrosion caused by body contact. An artificial sweat solution was used to simulate body contact. ALD alumina layers of varying thicknesses ranging from 20 to 80 nm were deposited on sputtered silver samples. The stability of the protective layer was evaluated by immersing the coated samples in the artificial sweat solution at 25 and 35 °C for 24 h. We confirmed that a sufficiently thick layer of ALD alumina is effective in protecting the shape and light reflectance of the underlying silver, whereas the uncoated bare silver is severely degraded by the artificial sweat solution. Inductively coupled plasma mass spectrometry and X-ray photoelectron spectroscopy were used for in-depth analyses of the chemical stability of the ALD-coated silver samples after immersion in the sweat solution.

78 FR 45907 - United States Standards for Grades of Frozen Vegetables

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-30

... standards covered by these revisions are: frozen asparagus, frozen lima beans, frozen speckled butter beans..., lima beans, speckled butter beans, cooked squash, summer squash, etc., and not other vegetables. AMS... document. United States Standards for Grades of Frozen Lima Beans Update address for AMS. Change ``U.S...

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly...

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 2 2013-04-01 2013-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly...

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 2 2012-04-01 2012-04-01 false âFresh,â âfreshly frozen,â âfresh frozen,â âfrozen fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly...

Cryopreservation of Autologous Blood (Red Blood Cells, Platelets and Plasma)

NASA Astrophysics Data System (ADS)

Ebine, Kunio

Prevention of post-transfusion hepatitis is still a problem in cardiovascular surgery. We initiated the cryopreservation of autologous blood for the transfusion in elective cardiovascular surgery since 1981. This study includes 152 surgical cases in which autologous frozen, allogeneic frozen, and/or allogeneic non-frozen blood were used. In the 152 surgical cases, there were 69 cases in which autologous blood only (Group I) was used; 12 cases with autologous and allogeneic frozen blood (Group II); 46 cases with autologous and allgeneic frozen plus allogeneic non-frozen blood (Group III); and 25 cases with allogeneic frozen plus allogeneic non-frozen blood (Group IV). No hepatitis developed in Groups I (0%) and II (0%), but there was positive hepatitis in Groups III (4.3%) and IV (8.0%) . In 357 cases of those who underwent surgery with allogeneic non-frozen whole blood during the same period, the incidence rate of hepatitis was 13.7% (49/357). Patients awaiting elective surgery can store their own blood in the frozen state. Patients who undergo surgery with the cryoautotransfusion will not produce any infections or immunologic reactions as opposed to those who undergo surgery with the allogeneic non-frozen blood.

Immunization against rodent malaria with cryopreserved irradiated sporozoites of Plasmodium berghei

DOE Office of Scientific and Technical Information (OSTI.GOV)

Orjih A.U.; Nussenzweig, R.S.

1980-05-01

The preparation and storage of Plasmodium berghai sporozoites for immunization purposes is described. The sporozoites were irradiated and frozen either at -75/sup 0/C or in liquid nitrogen. After various periods sporozoites were thawed and injected into A/J mice. At the end of the immunization period the animals were challenged with infective sporozoites of P. berghei and monitored for parasitemia. It was found that the storage did not appreciably alter the ability of the irradiated sporozoites to induce protective immunity in the recipient animals. The highest protection (80 to 100%) has induced with sporozoites maintained in 10% serum and stored atmore » -75/sup 0/C.« less

Formation mechanism of the graphite-rich protective layer in blast furnace hearths

NASA Astrophysics Data System (ADS)

Jiao, Ke-xin; Zhang, Jian-liang; Liu, Zheng-jian; Liu, Feng; Liang, Li-sheng

2016-01-01

A long campaign life of blast furnaces is heavily linked to the existence of a protective layer in their hearths. In this work, we conducted dissection studies and investigated damage in blast furnace hearths to estimate the formation mechanism of the protective layer. The results illustrate that a significant amount of graphite phase was trapped within the hearth protective layer. Furthermore, on the basis of the thermodynamic and kinetic calculations of the graphite precipitation process, a precipitation potential index related to the formation of the graphite-rich protective layer was proposed to characterize the formation ability of this layer. We determined that, under normal operating conditions, the precipitation of graphite phase from hot metal was thermodynamically possible. Among elements that exist in hot metal, C, Si, and P favor graphite precipitation, whereas Mn and Cr inhibit this process. Moreover, at the same hot-face temperature, an increase of carbon concentration in hot metal can shorten the precipitation time. Finally, the results suggest that measures such as reducing the hot-face temperature and increasing the degree of carbon saturation in hot metal are critically important to improve the precipitation potential index.

The principles of cryostratigraphy

NASA Astrophysics Data System (ADS)

French, Hugh; Shur, Yuri

2010-08-01

Cryostratigraphy adopts concepts from both Russian geocryology and modern sedimentology. Structures formed by the amount and distribution of ice within sediment and rock are termed cryostructures. Typically, layered cryostructures are indicative of syngenetic permafrost while reticulate and irregular cryostructures are indicative of epigenetic permafrost. 'Cryofacies' can be defined according to patterns of sediment characterized by distinct ice lenses and layers, volumetric ice content and ice-crystal size. Cryofacies can be subdivided according to cryostructure. Where a number of cryofacies form a distinctive cryostratigraphic unit, these are termed a 'cryofacies assemblage'. The recognition, if present, of (i) thaw unconformities, (ii) other ice bodies such as vein ice (ice wedges), aggradational ice and thermokarst-cave ('pool') ice, and (iii) ice, sand and gravelly pseudomorphs is also important in determining the nature of the freezing process, the conditions under which frozen sediment accumulates, and the history of permafrost.

Short-term Clinical Results of Manipulation Under Ultrasound-Guided Brachial Plexus Block in Patients with Idiopathic Frozen Shoulder and Diabetic Secondary Frozen Shoulder.

PubMed

Ando, Akira; Hamada, Junichiro; Hagiwara, Yoshihiro; Sekiguchi, Takuya; Koide, Masashi; Itoi, Eiji

2018-01-01

This study examined the effectiveness of manipulation under ultrasound-guided brachial plexus block in patients with recalcitrant idiopathic frozen shoulder and diabetic secondary frozen shoulder (diabetic frozen shoulder). Forty-four idiopathic frozen shoulders and 10 diabetic frozen shoulders with failed conservative treatment for at least 3 months were included in this study. The manipulation was performed under ultrasound-guided brachial plexus block and visual analogue scale, range of motion, and Constant scores were measured before manipulation and at the last follow-up examination. No major complications were observed during the procedure. Sufficient improvement was not obtained in two patients during the procedure and to avoid complications, the procedure was discontinued and subsequently arthroscopic capsular release was performed. Visual analogue scale, range of motion towards all directions, and Constant scores were significantly improved after the manipulation in both the idiopathic frozen shoulder and diabetic frozen shoulder groups, however the diabetic group showed inferior results compared with those of the idiopathic group. This manipulation was effective and shortened the duration of symptoms in most of the idiopathic and diabetic frozen shoulders without major complications during the procedure. Diabetic frozen shoulder showed inferior clinical results and difficulty in recovery in range of motion, which indicated that diabetic frozen shoulder should be discussed as a different entity.

Application of an Automated Fish Biomonitoring System at Old O-Field for Continuous Acute Toxicity Effluent Monitoring

DTIC Science & Technology

1996-05-01

Tommy R. Shedd, Henry S. Gardner , Robert A. Finch, and Robert C. Bishoff U.S. Army Biomedical Research and Development Laboratory Fort Detrick...Zeigler Bros., Inc., Gardners , PA) plus frozen brine shrimp (Living World, Virginia Pet Supply, Fairfax VA). Fish were not fed during the 2-week...Environmental Protection Agency, Washington, DC. 3. Diamond , J.M. and T.R.Shedd. 1995. Standard Guide for Ventilatory Behavioral Toxicology Testing

76 FR 20217 - Perishable Agricultural Commodities Act: Impact of Post-Default Agreements on Trust Protection...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

... N.Y., 362 F.3d 33 (2nd Cir. 2004); Patterson Frozen Foods, Inc. v. Crown Foods, Int'l, 307 F.3d 666, 667 (7th Cir. 2002); Greg Orchards Produce, Inc. v. P. Roncone J., 180 F.3d 888, 892 (7th Cir. 1999); Idahoan Fresh v. Advantage Produce, Inc., 157F.3d 197, 205 (3d Cir. 1998); In re Lombardo Fruit and...

Armenia, Azerbaijan, and Georgia: Political Developments and Implications for U.S. Interests

DTIC Science & Technology

2011-04-15

of the diplomats] to implement in Azerbaijan the experiments of Arab countries, some of them should be declared persona non grata.” An official of...protections for human rights, including by releasing two Internet bloggers jailed for hooliganism in November 2009 (they were released in November 2010).6...the “frozen Nagorno Karabakh conflict,” in which “heightened rhetoric and distrust on both sides and violent incidents along the Line of Contact

21 CFR 160.150 - Frozen egg whites.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen egg whites. 160.150 Section 160.150 Food... HUMAN CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.150 Frozen egg whites. (a) Frozen egg whites, frozen egg albumen is the food prepared by freezing...

21 CFR 160.190 - Frozen egg yolks.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen egg yolks. 160.190 Section 160.190 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.190 Frozen egg yolks. (a) Frozen egg yolks, frozen yolks is the food prepared by freezing egg yolks that...

21 CFR 160.190 - Frozen egg yolks.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen egg yolks. 160.190 Section 160.190 Food and... CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.190 Frozen egg yolks. (a) Frozen egg yolks, frozen yolks is the food prepared by freezing egg yolks that...

21 CFR 160.150 - Frozen egg whites.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen egg whites. 160.150 Section 160.150 Food... HUMAN CONSUMPTION EGGS AND EGG PRODUCTS Requirements for Specific Standardized Eggs and Egg Products § 160.150 Frozen egg whites. (a) Frozen egg whites, frozen egg albumen is the food prepared by freezing...

Vitamin E Analogue Improves Rabbit Sperm Quality during the Process of Cryopreservation through Its Antioxidative Action

PubMed Central

Zhu, Zhendong; Fan, Xiaoteng; Lv, Yinghua; Zhang, Nan; Fan, Chuning; Zhang, Pengfei; Zeng, Wenxian

2015-01-01

The process of cryopreservation results in high concentration of reactive oxygen species which is detrimental to spermatozoa. The aim of this study was to investigate whether addition of vitamin E analogue to freezing extender can facilitate the cryosurvival of spermatozoa in rabbits, and how vitamin E protects spermatozoa against damages during the process of preservation. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentrations of Trolox (a vitamin E analogue). The level of radical oxygen species (ROS) in spermatozoa that was exposed to Trolox was significantly lower than that of the control during each step of the process of preservation. The percentage of frozen-thawed spermatozoa with lipid peroxidation in the Trolox treatments was significantly lower than that of the control. The motility, intact acrosome, membrane integrity and mitochondrial potentials of the frozen-thawed spermatozoa in the treatment of 200 μM Trolox were significantly higher than those of the control. These observations suggest that addition of vitamin E to a freezing extender leads to higher integrity of acrosome, plasma membrane and mitochondrial membrane potential as well as higher motility. Vitamin E protects spermatozoa through its capacity to quench ROS accumulation and lipid peroxidation during the process of preservation. Addition of Trolox is recommended to facilitate the improvement of semen preservation for the rabbit breeding industry. PMID:26700473

Puncture detecting barrier materials

DOEpatents

Hermes, R.E.; Ramsey, D.R.; Stampfer, J.F.; Macdonald, J.M.

1998-03-31

A method and apparatus for continuous real-time monitoring of the integrity of protective barrier materials, particularly protective barriers against toxic, radioactive and biologically hazardous materials has been developed. Conductivity, resistivity or capacitance between conductive layers in the multilayer protective materials is measured by using leads connected to electrically conductive layers in the protective barrier material. The measured conductivity, resistivity or capacitance significantly changes upon a physical breach of the protective barrier material. 4 figs.

Puncture detecting barrier materials

DOEpatents

Hermes, Robert E.; Ramsey, David R.; Stampfer, Joseph F.; Macdonald, John M.

1998-01-01

A method and apparatus for continuous real-time monitoring of the integrity of protective barrier materials, particularly protective barriers against toxic, radioactive and biologically hazardous materials has been developed. Conductivity, resistivity or capacitance between conductive layers in the multilayer protective materials is measured by using leads connected to electrically conductive layers in the protective barrier material. The measured conductivity, resistivity or capacitance significantly changes upon a physical breach of the protective barrier material.

Four studies on effects of environmental factors on the quality of National Atmospheric Deposition Program measurements

USGS Publications Warehouse

Wetherbee, Gregory A.; Latysh, Natalie E.; Lehmann, Christopher M.B.; Rhodes, Mark F.

2011-01-01

Selected aspects of National Atmospheric Deposition Program / National Trends Network (NADP/NTN) protocols are evaluated in four studies. Meteorological conditions have minor impacts on the error in NADP/NTN sampling. Efficiency of frozen precipitation sample collection is lower than for liquid precipitation samples. Variability of NTN measurements is higher for relatively low-intensity deposition of frozen precipitation than for higher-intensity deposition of liquid precipitation. Urbanization of the landscape surrounding NADP/NTN sites is not affecting trends in wet-deposition chemistry data to a measureable degree. Five NADP siting criteria intended to preserve wet-deposition sample integrity have varying degrees of effectiveness. NADP siting criteria for objects within the 90 degrees cones and trees within the 120 degrees cones projected from the collector bucket to sky are important for protecting sample integrity. Tall vegetation, fences, and other objects located within 5 meters of the collectors are related to the frequency of visible sample contamination, indicating the importance of these factors in NADP siting criteria.

Factors affecting the musculoskeletal disorders of workers in the frozen food manufacturing factories in Thailand.

PubMed

Thetkathuek, Anamai; Meepradit, Parvena; Jaidee, Wanlop

2016-01-01

The purpose of this research was to study factors affecting musculoskeletal disorders. The sample population of the study was 528 factory workers from the frozen food industry, as well as a controlled group of 255 office workers. The samples were collected during interviews using the Nordic questionnaire to assess musculoskeletal disorders, and to assess the risk by the rapid upper limb assessment and rapid entire body assessment techniques. The findings of the study were that most symptoms were found in the dissecting department, higher than in the controlled group. The details of the symptoms were, accordingly: elbow pain (adjusted odds ratio, 35.1; 95% CI [17.4, 70.9]). Regarding the risk of alcohol drinking, workers were exposed to more risks when alcohol was consumed. It is suggested that workers' health should be monitored regularly. People who work in a cold environment should be encouraged to wear body protection and to avoid drinking.

Effects of egg yolk, glycerol and the freezing rate on the viability and acrosomal structures of frozen ram spermatozoa.

PubMed

Watson, P F; Martin, I C

1975-04-01

The influence of egg yolk, glycerol and the freezing rate on the survival of ram spermatozoa and on the structure of their acrosomes after freezing was investigated. Egg yolk was shown to be beneficial not only during chilling but also during freezing; of the levels examined, 1-5% gave the greatest protection. Although the presence of glycerol in the diluent improved the survival of spermatozoa, increasing concentrations produced significant deterioration of the acrosomes. With closely controlled linear cooling rates, no overall difference was detected in the survival of spermatozoa frozen at rates between 6 and 24 degrees C per min. However, a significant interaction between freezing rate and the inclusion of glycerol in the diluent showed that glycerol was less important at the highest freezing rate. A sudden cooling phase near to the freezing point following the release of the latent heat of fusion was not detrimental to spermatozoa.

Modelling high Arctic deep permafrost temperature sensitivity in Northeast Greenland based on experimental and field observations

NASA Astrophysics Data System (ADS)

Rasmussen, Laura Helene; Zhang, Wenxin; Hollesen, Jørgen; Cable, Stefanie; Hvidtfeldt Christiansen, Hanne; Jansson, Per-Erik; Elberling, Bo

2017-04-01

Permafrost affected areas in Greenland are expected to experience a marked temperature increase within decades. Most studies have considered near-surface permafrost sensitivity, whereas permafrost temperatures below the depths of zero annual amplitude is less studied despite being closely related to changes in near-surface conditions, such as changes in active layer thermal properties, soil moisture and snow depth. In this study, we measured the sensitivity of thermal conductivity (TC) to gravimetric water content (GWC) in frozen and thawed permafrost sediments from fine-sandy and gravelly deltaic and fine-sandy alluvial deposits in the Zackenberg valley, NE Greenland. We further calibrated a coupled heat and water transfer model, the "CoupModel", for one central delta sediment site with average snow depth and further forced it with meteorology from a nearby delta sediment site with a topographic snow accumulation. With the calibrated model, we simulated deep permafrost thermal dynamics in four 20-year scenarios with changes in surface temperature and active layer (AL) soil moisture: a) 3 °C warming and AL water table at 0.5 m depth; b) 3 °C warming and AL water table at 0.1 m depth; c) 6 °C warming and AL water table at 0.5 m depth and d) 6 °C warming and AL water table at 0.1 m depth. Our results indicate that frozen sediments have higher TC than thawed sediments. All sediments show a positive linear relation between TC and soil moisture when frozen, and a logarithmic one when thawed. Gravelly delta sediments were highly sensitive, but never reached above 12 % GWC, indicating a field effect of water retention capacity. Alluvial sediments are less sensitive to soil moisture than deltaic (fine and coarse) sediments, indicating the importance of unfrozen water in frozen sediment. The deltaic site with snow accumulation had 1 °C higher mean annual ground temperature than the average snow depth site. Permafrost temperature at the depth of 18 m increased with 1.5 °C and 3.5 °C in the scenarios with 3 °C and 6 °C warming, respectively. Increasing the soil moisture had no important additional effect to warming, although an increase in thermal offset was indicated. We conclude that below-ground sediment properties affect the sensitivity of TC to GWC, that surface temperature changes can influence the deep permafrost within a short time scale, and that differences in snow depth affect surface temperatures. Sediment type and the type of precipitation should thus be considered when estimating future High Arctic deep permafrost sensitivity.

Cross-layer shared protection strategy towards data plane in software defined optical networks

NASA Astrophysics Data System (ADS)

Xiong, Yu; Li, Zhiqiang; Zhou, Bin; Dong, Xiancun

2018-04-01

In order to ensure reliable data transmission on the data plane and minimize resource consumption, a novel protection strategy towards data plane is proposed in software defined optical networks (SDON). Firstly, we establish a SDON architecture with hierarchical structure of data plane, which divides the data plane into four layers for getting fine-grained bandwidth resource. Then, we design the cross-layer routing and resource allocation based on this network architecture. Through jointly considering the bandwidth resource on all the layers, the SDN controller could allocate bandwidth resource to working path and backup path in an economical manner. Next, we construct auxiliary graphs and transform the shared protection problem into the graph vertex coloring problem. Therefore, the resource consumption on backup paths can be reduced further. The simulation results demonstrate that the proposed protection strategy can achieve lower protection overhead and higher resource utilization ratio.

Assessing the Rationale and Effectiveness of Frozen Plasma Transfusions: An Evidence-based Review.

PubMed

Tinmouth, Alan

2016-06-01

Frozen plasma is a commonly used blood product. The primary indications for frozen plasma are the treatment and prevention of bleeding in patients with prolonged coagulation tests. However, there is a lack of well-conducted clinical trials to determine the appropriate indications for frozen plasma. The rationale and evidence for frozen plasma transfusions are reviewed, including the evidence or lack of evidence supporting common indications. Targeting indications in which frozen plasma transfusions are clearly not beneficial as supported by the current evidence provides an opportunity to improve the current use of frozen plasma and reduce adverse transfusion events. Copyright © 2016 Elsevier Inc. All rights reserved.

76 FR 11447 - Agency Information Collection Activities; Proposed Collection; Comment Request; Protection of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-02

... Protection regulations, the science of ozone layer depletion, and related topics. SUPPLEMENTARY INFORMATION... compliance with the Montreal Protocol on Substances that Deplete the Ozone Layer (Protocol) and the CAA.... obligations under Article 2H of the Montreal Protocol on Substances that Deplete the Ozone Layer (Protocol...

Analysis of ERS 1 synthetic aperture radar data of frozen lakes in northern Montana and implications for climate studies

USGS Publications Warehouse

Hall, Dorothy K.; Fagre, Daniel B.; Klasner, Fritz; Linebaugh, Gregg; Liston, Glen E.

1994-01-01

Lakes that freeze each winter are good indicators of regional climate change if key parameters, such as freeze-up and breakup date and maximum ice thickness, are measured over a decade-scale time frame. Synthetic aperture radar (SAR) satellite data have proven to be especially useful for measurement of climatologically significant parameters characteristic of frozen lakes. In this paper, five lakes in Glacier National Park, Montana, have been studied both in the field and using Earth Remote-Sensing Satellite (ERS) 1 SAR data during the 1992-1993 winter. The lakes are characterized by clear ice, sometimes with tubular or rounded bubbles, and often with a layer of snow ice on top of the clear ice. They are also often snow covered. Freeze-up is detected quite easily using ERS 1 SAR data as soon as a thin layer of ice forms. The effect of snow ice on the backscatter is thought to be significant but is, as yet, undetermined. On the five lakes studied, relative backscatter was found to increase with ice thickness until a maximum was reached in February. Breakup, an often ill-defined occurrence, is difficult to detect because surface water causes the SAR signal to be absorbed, thus masking the ice below. Comparison of the bubble structure of thaw lakes in northern Alaska with lakes in northern Montana has shown that the ice structure is quite different, and this difference may contribute to differential SAR signature evolution in the lakes of the two areas.

Accommodating permafrost in contaminant transport modeling, a preliminary approach to modify the TREECS modeling tools

NASA Astrophysics Data System (ADS)

Ryder, J. L.; Dortch, M. S.; Johnson, B. E.

2017-12-01

Efforts are underway to adapt TREECS (Training Range Environmental Evaluation and Characterization System) for use in arctic or subarctic conditions where the extent and duration of snowpack and frozen ground may influence the development and concentration of contaminant plumes. TREECS is a multi-media model designed to aid facility managers in the long term stewardship of Army properties. TREECS includes sub-models for mass loading, soil, vadose zone, aquifer, and stream transport. Potential changes to the sub-models to improve the ability to model contaminant transport in areas with permafrost include accurately representing the dissolution of contaminants over a wider range of temperatures, estimating snow depth and ablation for both the hydrology and thermal conditions, determining ground freeze/thaw state and an average active layer depth, a more precise method to estimate a vertical transport time to a water table, and a soil interflow routine that adapts for permafrost condition. In this presentation we will show three sub-model comparisons 1) the use of the National Weather Service SNOW-17 model and the current TREECS snowmelt routines for input hydrology, 2) a Continuous Frozen Ground Index (CFGI) model and the Geophysical Institute Permafrost Lab model (GIPL 1.0) for determining active layer depth and summer season length, and 3) the use of HYDRUS-1D and the current TREECS vadose zone model for transport to the water table. The performance vs input needs, assumptions, and limitations of each approach, as well as the physical system uncertainties will also be discussed.

High resolution track etch autoradiography

DOEpatents

Solares, G.; Zamenhof, R.G.

1994-12-27

A detector assembly is disclosed for use in obtaining alpha-track autoradiographs, the detector assembly including a substantially boron-free substrate; a detector layer deposited on the substantially boron-free substrate, the detector layer being capable of recording alpha particle tracks and exhibiting evidence of the alpha tracks in response to being exposed to an etchant, the detector layer being less than about 2 microns thick; and a protective layer deposited on the detector layer, the protective layer being resistant to the etchant and having a thickness of about 0.5 to 1 microns. 13 figures.

High resolution track etch autoradiography

DOEpatents

Solares, Guido; Zamenhof, Robert G.

1994-01-01

A detector assembly for use in obtaining alpha-track autoradiographs, the detector assembly including a substantially boron-free substrate; a detector layer deposited on the substantially boron-free substrate, the detector layer being capable of recording alpha particle tracks and exhibiting evidence of the alpha tracks in response to being exposed to an etchant, the detector layer being less than about 2 microns thick; and a protective layer deposited on the detector layer, the protective layer being resistant to the etchant and having a thickness of about 0.5 to 1 microns.

Frozen Section Evaluation of Margin Status in Primary Squamous Cell Carcinomas of the Head and Neck: A Correlation Study of Frozen Section and Final Diagnoses.

PubMed

Layfield, Eleanor M; Schmidt, Robert L; Esebua, Magda; Layfield, Lester J

2018-06-01

Frozen section is routinely used for intraoperative margin evaluation in carcinomas of the head and neck. We studied a series of frozen sections performed for margin status of head and neck tumors to determine diagnostic accuracy. All frozen sections for margin control of squamous carcinomas of the head and neck were studied from a 66 month period. Frozen and permanent section diagnoses were classified as negative or malignant. Correlation of diagnoses was performed to determine accuracy. One thousand seven hundred and ninety-six pairs of frozen section and corresponding permanent section diagnoses were obtained. Discordances were found in 55 (3.1%) pairs. In 35 pairs (1.9%), frozen section was reported as benign, but permanent sections disclosed carcinoma. In 21 cases, the discrepancy was due to sampling and in the remaining cases it was an interpretive error. In 20 cases (1.1%), frozen section was malignant, but the permanent section was interpreted as negative. Frozen section is an accurate method for evaluation of operative margins for head and neck carcinomas with concordance between frozen and permanent results of 97%. Most errors are false negative results with the majority of these being due to sampling issues.

21 CFR 161.175 - Frozen raw breaded shrimp.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen raw breaded shrimp. 161.175 Section 161.175... § 161.175 Frozen raw breaded shrimp. (a) Frozen raw breaded shrimp is the food prepared by coating one..., other than those provided for in this paragraph, are not suitable ingredients of frozen raw breaded...

21 CFR 161.175 - Frozen raw breaded shrimp.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen raw breaded shrimp. 161.175 Section 161.175... § 161.175 Frozen raw breaded shrimp. (a) Frozen raw breaded shrimp is the food prepared by coating one..., other than those provided for in this paragraph, are not suitable ingredients of frozen raw breaded...

Inorganic-Organic Coating via Molecular Layer Deposition Enables Long Life Sodium Metal Anode.

PubMed

Zhao, Yang; Goncharova, Lyudmila V; Zhang, Qian; Kaghazchi, Payam; Sun, Qian; Lushington, Andrew; Wang, Biqiong; Li, Ruying; Sun, Xueliang

2017-09-13

Metallic Na anode is considered as a promising alternative candidate for Na ion batteries (NIBs) and Na metal batteries (NMBs) due to its high specific capacity, and low potential. However, the unstable solid electrolyte interphase layer caused by serious corrosion and reaction in electrolyte will lead to big challenges, including dendrite growth, low Coulombic efficiency and even safety issues. In this paper, we first demonstrate the inorganic-organic coating via advanced molecular layer deposition (alucone) as a protective layer for metallic Na anode. By protecting Na anode with controllable alucone layer, the dendrites and mossy Na formation have been effectively suppressed and the lifetime has been significantly improved. Moreover, the molecular layer deposition alucone coating shows better performances than the atomic layer deposition Al 2 O 3 coating. The novel design of molecular layer deposition protected Na metal anode may bring in new opportunities to the realization of the next-generation high energy-density NIBs and NMBs.

Atomic layer deposited oxide films as protective interface layers for integrated graphene transfer

NASA Astrophysics Data System (ADS)

Cabrero-Vilatela, A.; Alexander-Webber, J. A.; Sagade, A. A.; Aria, A. I.; Braeuninger-Weimer, P.; Martin, M.-B.; Weatherup, R. S.; Hofmann, S.

2017-12-01

The transfer of chemical vapour deposited graphene from its parent growth catalyst has become a bottleneck for many of its emerging applications. The sacrificial polymer layers that are typically deposited onto graphene for mechanical support during transfer are challenging to remove completely and hence leave graphene and subsequent device interfaces contaminated. Here, we report on the use of atomic layer deposited (ALD) oxide films as protective interface and support layers during graphene transfer. The method avoids any direct contact of the graphene with polymers and through the use of thicker ALD layers (≥100 nm), polymers can be eliminated from the transfer-process altogether. The ALD film can be kept as a functional device layer, facilitating integrated device manufacturing. We demonstrate back-gated field effect devices based on single-layer graphene transferred with a protective Al2O3 film onto SiO2 that show significantly reduced charge trap and residual carrier densities. We critically discuss the advantages and challenges of processing graphene/ALD bilayer structures.

Influence of microclimate on the sustainability and reliability of weathering steel bridge

NASA Astrophysics Data System (ADS)

Kubzova, M.; Krivy, V.; Kreislova, K.

2018-04-01

Reliability and sustainability of bridge structures designed from weathering steel are influenced by the development of a sufficiently protective layer of corrosion products on its surface. The development of this protective layer is affected by several parameters such as air pollution around the bridge structure, the microclimate under the bridge, the location of surface within the bridge structure and the time of wetness. Design of structural details also significantly influences the development of the protective corrosion layer. The article deals with the results of the experimental tests carried out on the road bridge located in the city of Ostrava in the Czech Republic. The development of the protective corrosion layer on the surface of the bridge is significantly influenced by the intensive traffic under the bridge construction and the design solution of the bridge itself. Attention is focused mainly on the influence of chloride deposition on the protective function of the corrosion layer. Corrosion samples were placed on the bridge to evaluate the influence of the above-mentioned parameters. The deposition rate of chlorides spreading from the road to surfaces of the steel structure is also measured.

Fuel tank for liquefied natural gas

NASA Technical Reports Server (NTRS)

DeLay, Thomas K. (Inventor)

2012-01-01

A storage tank is provided for storing liquefied natural gas on, for example, a motor vehicle such as a bus or truck. The storage tank includes a metal liner vessel encapsulated by a resin-fiber composite layer. A foam insulating layer, including an outer protective layer of epoxy or of a truck liner material, covers the composite layer. A non-conducting protective coating may be painted on the vessel between the composite layer and the vessel so as to inhibit galvanic corrosion.

Deuterium-Tritium Beta-Layering Within a National Ignition Facility Scale Polymer Target in the LANL Cryogenic Pressure Loader

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ebey, Peter S.; Dole, James M.; Geller, Drew A.

2005-11-15

Beta-layering, the process of beta-decay heat-driven mass redistribution, has been demonstrated in a deuterium-tritium (D-T)-filled polymer sphere of the type required for fusion ignition experiments at the National Ignition Facility. This is the first report, to the best of the authors' knowledge, of a D-T layer formed in a permeation-filled sphere. The 2-mm-diam sphere was filled with D-T by permeation; cooled to cryogenic temperatures while in the high-pressure permeation vessel; and, while cold, removed to an optical axis where the D-T was frozen, melted, and beta-layered in a series of experiments over several weeks' time. This work was performed inmore » the Los Alamos National Laboratory cryogenic pressure loader system. The beta-layering time constant was 24.0 {+-} 2.5 min, less than the theoretical value of 26.8 min, and not showing the significant increase due to build-up of {sup 3}He often observed in beta-layered samples. Supercooling of the liquid D-T was observed. Neither the polymer target nor its tenting material showed visual signs of degradation after 5 weeks of exposure to D-T. Small external thermal gradients were used to shift the D-T material back and forth within the sphere.« less

Classification of permafrost active layer depth from remotely sensed and topographic evidence

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peddle, D.R.; Franklin, S.E.

1993-04-01

The remote detection of permafrost (perennially frozen ground) has important implications to environmental resource development, engineering studies, natural hazard prediction, and climate change research. In this study, the authors present results from two experiments into the classification of permafrost active layer depth within the zone of discontinuous permafrost in northern Canada. A new software system based on evidential reasoning was implemented to permit the integrated classification of multisource data consisting of landcover, terrain aspect, and equivalent latitude, each of which possessed different formats, data types, or statistical properties that could not be handled by conventional classification algorithms available to thismore » study. In the first experiment, four active layer depth classes were classified using ground based measurements of the three variables with an accuracy of 83% compared to in situ soil probe determination of permafrost active layer depth at over 500 field sites. This confirmed the environmental significance of the variables selected, and provided a baseline result to which a remote sensing classification could be compared. In the second experiment, evidence for each input variable was obtained from image processing of digital SPOT imagery and a photogrammetric digital elevation model, and used to classify active layer depth with an accuracy of 79%. These results suggest the classification of evidence from remotely sensed measures of spectral response and topography may provide suitable indicators of permafrost active layer depth.« less

The effect of the osmolality of sugar-containing media, the type of sugar, and the mass and molar concentration of sugar on the survival of frozen-thawed mouse sperm.

PubMed

Koshimoto, Chihiro; Mazur, Peter

2002-08-01

Several factors have contributed to problems in mouse sperm cryopreservation, and we and others have found ways to ameliorate them. These include high sensitivity to several types of mechanical stresses and to oxygen-derived free radicals, low tolerance to osmotic cell volume changes, and rather rigorous requirements for cooling and warming rates. Another important factor is the cryoprotective agent. Mouse sperm are unusual in that our best results have been obtained in media containing the nonpermeating sugar raffinose (18% w/v) and lacking glycerol. This paper deals with questions about the basis of the protective action of sugars, and whether raffinose is unusual or unique in its ability to confer protection. More specifically, we investigated whether protection was more related to the total osmolality of the freezing solution, to the mass concentration of sugar, or to the molarity of the sugar, and we looked to see whether there are effects attributable to specific sugars. To investigate these questions, mouse sperm were frozen at the optimal rate of 25 degrees C/min in solutions prepared with different proportions of three sugars-raffinose, sucrose, and glucose-dissolved in 1/4x PBS. In the first experimental series, the total osmolality and the total sugar molarity were varied from 400 to 700 mOsm and from 300 to 530 mM, respectively, while holding the mass concentration of sugar constant at 18% (w/v). In the second experimental series, the mass concentration of sugars was varied from 10 to 18% while the sugar molarity and solution osmolality remained constant at 300 mM and 420 mOsm, respectively. The results suggest that protection against freezing and thawing depends more on the mass concentration of the sugar than on its molar concentration, a conclusion that has mechanistic implications.

Infrared thermography of the pig thorax: an assessment of selected regions of interest by computed tomographical and anatomical parameters.

PubMed

Menzel, A; Siewert, C; Gasse, H; Seifert, H; Hoeltig, D; Hennig-Pauka, I

2015-04-01

Current methods of diagnosis of respiratory diseases in swine are invasive, time-consuming and expensive. Infrared thermography (IRT) of the thorax might provide a new method of high specificity to select swine affected with lung alterations for further diagnostics. In this study, layer thickness of different tissues was determined in frozen thorax slices (FTS) by computed tomography (CT) and then related to skin temperatures measured by IRT in healthy pigs. The aim was to determine appropriate regions of interest (ROI) for evaluation of IRT images. Organ layer thicknesses measured in CT images correspond to those measured in FTS. Temperature differences between lung ROIs and abdomen ROIs were positively correlated with lung layer thickness at certain localizations, and negatively correlated with the thickness of the thorax wall and of inner organ layers. Reference values of differences between skin temperatures were established for two ROIs on the thorax with potential practical use for lung health status determination. Respective ROIs were located on vertical lines crossing the 7th (right) and the 10th (left) thoracic vertebrae. The presence of ribs affected skin temperature significantly. © 2014 Blackwell Verlag GmbH.

The Hydromagnetic Interior of a Solar Quiescent Prominence. I. Coupling between Force Balance and Steady Energy Transport

NASA Astrophysics Data System (ADS)

Low, B. C.; Berger, T.; Casini, R.; Liu, W.

2012-08-01

This series of papers investigates the dynamic interiors of quiescent prominences revealed by recent Hinode and SDO/AIA high-resolution observations. This first paper is a study of the static equilibrium of the Kippenhahn-Schlüter diffuse plasma slab, suspended vertically in a bowed magnetic field, under the frozen-in condition and subject to a theoretical thermal balance among an optically thin radiation, heating, and field-aligned thermal conduction. The everywhere-analytical solutions to this nonlinear problem are an extremely restricted subset of the physically admissible states of the system. For most values of the total mass frozen into a given bowed field, force balance and steady energy transport cannot both be met without a finite fraction of the total mass having collapsed into a cold sheet of zero thickness, within which the frozen-in condition must break down. An exact, resistive hydromagnetic extension of the Kippenhahn-Schlüter slab is also presented, resolving the mass-sheet singularity into a finite-thickness layer of steadily falling dense fluid. Our hydromagnetic result suggests that the narrow, vertical prominence Hα threads may be falling across magnetic fields, with optically thick cores much denser and ionized to much lower degrees than conventionally considered. This implication is discussed in relation to (1) the recent SDO/AIA observations of quiescent prominences that are massive and yet draining mass everywhere in their interiors, (2) the canonical range of 5-60 G determined from spectral polarimetric observations of prominence magnetic fields over the years, and (3) the need for a more realistic multi-fluid treatment.

Lipid class and fatty acid composition of a little-known and rarely collected alga Exophyllum wentii Weber-van Bosse from Bali Island, Indonesia.

PubMed

Illijas, Muhammad I; Indy, Jeane R; Yasui, Hajime; Itabashi, Yutaka

2009-01-01

The lipid class and fatty acid composition of a little-known and rarely collected alga Exophyllum wentii from Bali Island, Indonesia were determined for fresh and frozen-thawed samples using thin-layer chromatography, gas-liquid chromatography, and high-performance liquid chromatography. Glycoglycerolipids, which mainly consisted of mongalactosyldiacylglycerols (MGDG) and digalactosyldiacylglycerols (DGDG), were the predominant lipid components, accounting for 67% and 56% of the total polar lipid content in the fresh and frozen-thawed samples, respectively. Phospholipids, including phosphatidylcholines (PC) and phosphatidylglycerols (PG), were detected with lesser amounts in both samples (16-17% of the total polar lipid content). Free fatty acids (FFA), sterols and triacylglycerols (TAG) were also detected in minor quantities; however, the FFA content in the frozen-thawed sample increased to up to 20% of the total lipid content, suggesting that hydrolysis of the membrane lipids had occurred. A crude enzyme preparation from the alga showed activities for hydrolyzing the acyl groups of the phospholipids and glycoglycerolipids. Palmitic acid (16:0) and arachidonic acid (20:4n-6) were the major fatty acids in both the total lipid and in individual polar lipid classes as well as the dominant fatty acids released from the membrane lipids by enzymatic hydrolysis. The high level of 20:4n-6 (29%) in the total lipid and the presence of considerable amounts of PC (11% of the total polar lipid) and PG (6.2%) support classification of E. wentii into the Division Rhodophyta.

Simultaneous enhancement of photovoltage and charge transfer in Cu{sub 2}O-based photocathode using buffer and protective layers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Changli; Delaunay, Jean-Jacques, E-mail: jean@mech.t.u-tokyo.ac.jp; Hisatomi, Takashi

2016-07-18

Coating n-type buffer and protective layers on Cu{sub 2}O may be an effective means to improve the photoelectrochemical (PEC) water-splitting performance of Cu{sub 2}O-based photocathodes. In this letter, the functions of the buffer layer and protective layer on Cu{sub 2}O are examined. It is found that a Ga{sub 2}O{sub 3} buffer layer can form a buried junction with Cu{sub 2}O, which inhibits Cu{sub 2}O self-reduction as well as increases the photovoltage through a small conduction band offset between the two semiconductors. The introduction of a TiO{sub 2} thin protective layer not only improves the stability of the photocathode but alsomore » enhances the electron transfer from the photocathode surface into the electrolyte, thus resulting in an increase in photocurrent at positive potentials. These results show that the selection of overlayers with appropriate conduction band positions provides an effective strategy for obtaining a high photovoltage and high photocurrent in PEC systems.« less

Effect of gamma-irradiation on frozen shrimps for decontamination of pathogenic bacteria

NASA Astrophysics Data System (ADS)

Ito, Hitoshi; Rashid, Harun Or; Sangthong, Naruemon; Adulyatham, Pitaya; Rattagool, Pongpen; Ishigaki, Isao

1993-07-01

Twelve samples of imported frozen shrimps were used in this study. The total aerobic bacteria were at 2 × 10 4 to 6 × 10 6 per gram. A few of Vibrio parahaemolyticus, V. mimicus, V. alginolyticus, V. vulnificus, V. fluvialis and Listeria monocytogenes were isolated from many samples. However, Salmonella was not detected in any of the samples. After exposure to 4-5 kGy of gamma-rays, the total aerobic bacteria in frozen shrimps were reduced by approximately 2-3 log cycles. The dose necessary to reduce the vibrio isolates and Aeromonas hydrophila at a level of below 10 -4 per gram was about 3 kGy in frozen shrimps, whereas about 3.5 kGy was required for L. monocytogenes and Salmonella typhimurium. In this study, unpleasant off-odor was clearly detected in the non-frozen shrimps irradiated at 2.5 kGy. On the other hand, off-odor was negligible in the frozen product below 5 kGy irradiation. No remarkable changes of peroxide values were also obtained up to 9 kGy of irradiation in the frozen shrimps. However peroxide values of non-frozen shrimps were clearly increased even irradiated at 4 kGy. Trimethylamine content was not changed at doses below 10 kGy in both of frozen and non-frozen shrimps. Shelf-life of defrosted shrimps were extended ca. 2 times under non-frozen market conditions.

A global, open-source database of flood protection standards

NASA Astrophysics Data System (ADS)

Scussolini, Paolo; Aerts, Jeroen; Jongman, Brenden; Bouwer, Laurens; Winsemius, Hessel; de Moel, Hans; Ward, Philip

2016-04-01

Accurate flood risk estimation is pivotal in that it enables risk-informed policies in disaster risk reduction, as emphasized in the recent Sendai framework for Disaster Risk Reduction. To improve our understanding of flood risk, models are now capable to provide actionable risk information on the (sub)global scale. Still the accuracy of their results is greatly limited by the lack of information on standards of protection to flood that are actually in place; and researchers thus take large assumptions on the extent of protection. With our work we propose a first global, open-source database of FLOod PROtection Standards, FLOPROS, covering a range of spatial scales. FLOPROS is structured in three layers of information, and merges them into one consistent database: 1) the Design layer contains empirical information about the standard of protection presently in place; 2) the Policy layer contains intended protection standards from normative documents; 3) the Model layer uses a validated numerical approach to calculate protection standards for areas not covered in the other layers. The FLOPROS database can be used for more accurate risk assessment exercises across scales. As the database should be continually updated to reflect new interventions, we invite researchers and practitioners to contribute information. Further, we look for partners within the risk community to participate in additional strategies to implement the amount and accuracy of information contained in this first version of FLOPROS.

A Limited Survey of Heavy Metal Concentrations in Fresh and Frozen Cuttlefish Ink and Mantle Used As Food.

PubMed

Conficoni, Daniele; Alberghini, Leonardo; Bissacco, Elisa; Contiero, Barbara; Giaccone, Valerio

2018-02-01

Cuttlefish ink is consumed as a delicacy worldwide. The current study is the first assessment of heavy metal concentrations in cuttlefish ink versus mantle under different storage methods. A total of 212 samples (64 of fresh mantle, 42 of frozen mantle, 64 of fresh ink, and 42 of frozen ink) were analyzed for the detection of the following heavy metals: arsenic (As), chromium (Cr), iron (Fe), lead (Pb), mercury (Hg), and cadmium (Cd). The median As concentrations were 12.9 mg/kg for fresh mantle, 8.63 mg/kg for frozen mantle, 10.8 mg/kg for frozen ink, and 0.41 mg/kg for fresh ink. The median Cr concentrations were 0.06 mg/kg for fresh mantle and frozen ink, 0.03 mg/kg for frozen mantle, and below the limit of quantification (LOQ) for fresh ink. The median Fe concentrations were 4.08 mg/kg for frozen ink, 1.51 mg/kg for fresh mantle, 0.73 mg/kg for frozen mantle, and below the LOQ for fresh ink. The median Pb concentrations of almost all samples were below the LOQ; only two frozen ink, one fresh ink, one frozen mantle, and one fresh mantle sample exceeded the limit stipulated by the European Union. The Hg concentrations were statistically similar among the four categories of samples; the median Hg concentrations were below the LOQ, and the maximum concentrations were found in frozen ink, at 1.62 mg/kg. The median Cd concentrations were 0.69 mg/kg for frozen ink and 0.11 mg/kg for frozen mantle, fresh mantle and fresh ink concentrations were below the LOQ, and in 11.3% of the tested samples, Cd concentrations were higher than the European Union limit. The probability of samples having a Cd concentration above the legal limit was 35.75 times higher in frozen than in fresh products. Fresh ink had significantly lower concentrations of As, Cr, Fe, and Cd, but the concentrations of Hg and Pb were not significantly different from those of other products. Frozen ink had significantly higher concentrations of Cd, Cr, and Fe, but concentrations of As were lower than those in fresh mantle, pointing out a possible role for the freezing process and for different fishing zones as risk factors for heavy metal contamination.

Metal Surface Modification for Obtaining Nano- and Sub-Nanostructured Protective Layers.

PubMed

Ledovskykh, Volodymyr; Vyshnevska, Yuliya; Brazhnyk, Igor; Levchenko, Sergiy

2017-12-01

Regularities of the phase protective layer formation in multicomponent systems involving inhibitors with different mechanism of protective action have been investigated. It was shown that optimization of the composition of the inhibition mixture allows to obtain higher protective efficiency owing to improved microstructure of the phase layer. It was found that mechanism of the film formation in the presence of NaNO 2 -PHMG is due to deposition of slightly soluble PHMG-Fe complexes on the metal surface. On the basis of the proposed mechanism, the advanced surface engineering methods for obtaining nanoscaled and sub-nanostructured functional coatings may be developed.

Metal Surface Modification for Obtaining Nano- and Sub-Nanostructured Protective Layers

NASA Astrophysics Data System (ADS)

Ledovskykh, Volodymyr; Vyshnevska, Yuliya; Brazhnyk, Igor; Levchenko, Sergiy

2017-03-01

Regularities of the phase protective layer formation in multicomponent systems involving inhibitors with different mechanism of protective action have been investigated. It was shown that optimization of the composition of the inhibition mixture allows to obtain higher protective efficiency owing to improved microstructure of the phase layer. It was found that mechanism of the film formation in the presence of NaNO2-PHMG is due to deposition of slightly soluble PHMG-Fe complexes on the metal surface. On the basis of the proposed mechanism, the advanced surface engineering methods for obtaining nanoscaled and sub-nanostructured functional coatings may be developed.

Theoretical and Computational Studies of Stability, Transition and Flow Control in High-Speed Flows

DTIC Science & Technology

2011-02-22

A. H. Nayfeh. Nonparallel stability of boundary layers with pressure gradients and suction. Technical Report AGARD - CP -224, 1977. [Squ33] H. B. Squire...only. µ = µr ( T Tr )3/2 Tr + Ts T + Ts , (2.13) 8 K = µcp Pr , (2.14) where µr = 1.7894 × 10−5 Ns/m2, Tr = 288.0 K, Ts = 110.33 K, and cp is the...fraction of species s Cpf = frozen specific heat, cal/g-mole-K Cp ,s = specific heat at constant pressure of species s, cal/g-mole Dij = binary diffusion

Anti-contamination device for cryogenic soft X-ray diffraction microscopy

DOE PAGES

Huang, Xiaojing; Miao, Huijie; Nelson, Johanna; ...

2011-05-01

Cryogenic microscopy allows one to view frozen hydrated biological and soft matter specimens with good structural preservation and a high degree of stability against radiation damage. We describe a liquid nitrogen-cooled anti-contamination device for cryogenic X-ray diffraction microscopy. The anti-contaminator greatly reduces the buildup of ice layers on the specimen due to condensation of residual water vapor in the experimental vacuum chamber. We show by coherent X-ray diffraction measurements that this leads to fivefold reduction of background scattering, which is important for far-field X-ray diffraction microscopy of biological specimens.

Protection layers on a superconducting microwave resonator toward a hybrid quantum system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Jongmin, E-mail: jongmin.lee@sandia.gov; Sandia National Laboratories, Albuquerque, New Mexico 87123; Park, Dong Hun, E-mail: leomac@umd.edu

2015-10-07

We propose a protection scheme of a superconducting microwave resonator to realize a hybrid quantum system, where cold neutral atoms are coupled with a single microwave photon through magnetic dipole interaction at an interface inductor. The evanescent field atom trap, such as a waveguide/nanofiber atom trap, brings both surface-scattered photons and absorption-induced broadband blackbody radiation which result in quasiparticles and a low quality factor at the resonator. A proposed multiband protection layer consists of pairs of two dielectric layers and a thin nanogrid conductive dielectric layer above the interface inductor. We show numerical simulations of quality factors and reflection/absorption spectra,more » indicating that the proposed multilayer structure can protect a lumped-element microwave resonator from optical photons and blackbody radiation while maintaining a reasonably high quality factor.« less

76 FR 13429 - Notice of Availability of Final Environmental Impact Statement for the Proposed Blackfoot Bridge...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-11

... 1B would incorporate a layer of impermeable material called a laminated geosynthetic clay liner, or... inches of topsoil; 1 foot of weathered alluvium cover material; 6 inches of drainage/protective layer material; GCLL; 6 inches of a protective sub-grade layer (weathered alluvium or other earthen material...

DOE Office of Scientific and Technical Information (OSTI.GOV)

James, S. R.; Knox, H. A.; Abbott, R. E.

Cross correlations of seismic noise can potentially record large changes in subsurface velocity due to permafrost dynamics and be valuable for long-term Arctic monitoring. We applied seismic interferometry, using moving window cross-spectral analysis (MWCS), to 2 years of ambient noise data recorded in central Alaska to investigate whether seismic noise could be used to quantify relative velocity changes due to seasonal active-layer dynamics. The large velocity changes (>75%) between frozen and thawed soil caused prevalent cycle-skipping which made the method unusable in this setting. We developed an improved MWCS procedure which uses a moving reference to measure daily velocity variationsmore » that are then accumulated to recover the full seasonal change. This approach reduced cycle-skipping and recovered a seasonal trend that corresponded well with the timing of active-layer freeze and thaw. Lastly, this improvement opens the possibility of measuring large velocity changes by using MWCS and permafrost monitoring by using ambient noise.« less

Sperm characteristics and heterologous in vitro fertilisation capacity of Iberian ibex (Capra pyrenaica) epididymal sperm, frozen in the presence of the enzymatic antioxidant catalase.

PubMed

López-Saucedo, J; Paramio, M T; Fierro, R; Izquierdo, D; Catalá, M G; Coloma, M A; Toledano-Díaz, A; López-Sebastián, A; Santiago-Moreno, J

2014-06-01

The aim of this work was to evaluate the protective effect of catalase (CAT) on frozen/thawed ibex epididymal sperm recovered post mortem, and to detect any harmful effect this might have on sperm fertilisation capacity. Epididymal spermatozoa were diluted using a Tris-citric acid-glucose medium (TCG) composed of 3.8% Tris (w/v), 2.2% citric acid (w/v), 0.6% glucose (w/v), 5% glycerol (v/v), and 6% egg yolk (v/v). Sperm masses from the right epididymis were diluted with TCG medium, while those from the left were diluted with TCG medium supplemented with 200IU/mL CAT. Heterologous in vitro fertilisation (IVF) was used to assess the fertilisation capacity of this sperm. The addition of CAT to the extender did not improve frozen/thawed sperm variables. Moreover, a reduced fertilisation capacity was detected: sperm diluted with TCG provided 25.5% 2PN zygotes, while just 13.2% was recorded for that diluted with TCG-CAT (P<0.01). The percentage of cleaved embryos at 48hpi was higher (P<0.01) with the TCG sperm than with the TCG-CAT sperm (16.7% vs. 7.6%). The use of 200IU/mL CAT as an additive cannot, therefore, be recommended for the preservation of ibex epididymal sperm. Other antioxidants should, however, be tested in both this and related wild mountain ungulates. Copyright © 2014 Elsevier Inc. All rights reserved.

Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa.

PubMed

Nicolas, M; Alvarez, M; Borragán, S; Martinez-Pastor, F; Chamorro, C A; Alvarez-Rodriguez, M; de Paz, P; Anel, L

2012-04-01

Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample. Copyright © 2012 Elsevier Inc. All rights reserved.

Rhodiola sacra aqueous extract (RSAE) improves biochemical and sperm characteristics in cryopreserved boar semen.

PubMed

Zhao, H-W; Li, Q-W; Ning, G-Z; Han, Z-S; Jiang, Z-L; Duan, Y-F

2009-03-15

Although Rhodiola sacra aqueous extract (RSAE) has been used in many studies as an antioxidant, its effects on semen characteristics and its antioxidant properties during cryopreservation of boar sperm have never been evaluated. Semen was collected from five Duroc boars (2-4-year-old) twice weekly and frozen-thawed in extender with RSEA. Motion characteristics were assessed with a computer-aided semen analysis (CASA) system, whereas other sperm quality end points were assessed by routine methods. The effective concentration of RSEA in extender ranged from 4 to 8mg/L and the effect of RSEA on sperm quality was better in glycerol-free extender than extender containing glycerol (P<0.05). In frozen-thawed boar semen, there was a direct correlation (P<0.05) between RSEA concentration and glutathione (GSH) concentrations, mitochondrial activity, and hypoosmotic swelling test (HOST), and an inverse correlation (r=-0.982, P<0.05) between RSEA concentration and malondialdehyde (all end points were significantly higher at 6mg/L than in the control group). In summary: (i) the effective concentration of RSEA in extender ranged from 4 to 8mg/L; (ii) the effect of RSEA on sperm quality was better in extender without glycerol; and (iii) there was a significant correlation between RSEA concentrations and concentrations of GSH and MAD in frozen-thawed boar semen (antioxidant effects of RSEA were concentration-dependent). Further studies are needed to define the active ingredient in RSEA that protects boar sperm against ROS.

Effect of ethanolic flax (Linum usitatissimum L.) extracts on lipid oxidation and changes in nutritive value of frozen-stored meat products.

PubMed

Waszkowiak, Katarzyna; Szymandera-Buszka, Krystyna; Hęś, Marzanna

2014-01-01

Flaxseed (Linum usitatissimum L.) is an important source of phenolic compounds, mainly lignans. Antioxidant capacities of flaxseed extracts that contain the compounds have been reported earlier. However, there is a lack of accessible information about their activity against lipid oxidation in meat products. Therefore, the effect of ethanolic flaxseed extracts (EFEs) on lipid stability and changes in nutritive value of frozen-stored meat products (pork meatballs and burgers) was determined. EFEs from three Polish flax varieties (Szafir, Oliwin, Jantarol) were applied in the study. During 150-day storage of meat products, the lipid oxidation (peroxide and TBARS value) and thiamine retention were periodically monitored, alongside with methionine and lysine availability and protein digestibility. The addition of EFEs significantly limited lipid oxidation in stored meatballs and burgers. EFE from brown seeds of Szafir var. was superior to the others from golden seeds of Jantarol and Oliwin. Moreover, the extracts reduced changes in thiamine and available lysine content, as well as protein digestibility, during storage time. The effect of EFE addition on available methionine retention was limited. The ethanolic flaxseed extracts exhibit antioxidant activity during frozen storage of meat products. They can be utilized to prolong shelf-life of the products by protecting them against lipid oxidation and deterioration of their nutritional quality. However, antioxidant efficiency of the extracts seems to depend on chemical composition of raw material (flax variety). Further investigations should be carried on to explain the issue.

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa.

PubMed

Neuhauser, S; Dörfel, S; Handler, J

2015-05-01

Preservation of epididymal spermatozoa is important to save genetic material of endangered species and breeds, or in case of unexpected injury, which will end the breeding career of valuable sires. Seminal plasma (SP) influences sperm quality in a dose-dependent manner and its addition to preserved semen immediately before insemination may be beneficial for sperm fertility. Increased plasma membrane stability of epididymal spermatozoa reduces freezing injury of cells, and the addition of SP after freezing and thawing might have activating and protecting effects on spermatozoa within the female genital tract. In this study, epididymal spermatozoa were harvested by retrograde flush of the epididymal cauda immediately after routine castration and frozen. Seminal plasma was collected from other six stallions. Homologous SP (SP from the same species, but from a different animal) was added to frozen-thawed epididymal spermatozoa at concentrations of 0, 5, 20, 50 and 80% SP. Addition of SP increased sperm motility and influenced kinematic values in a dose-dependent manner (p < 0.05). Motility improved at concentrations of 20 and 50% SP, but did not further increase at 80% SP. There was no difference in sperm motility among SP from six different donor stallions regardless of the concentrations of SP (p > 0.05). Total and progressive motility of ten frozen-thawed epididymal spermatozoa samples collected from different stallions after dilution with extender and 5, 20, 50 or 80% SP differed significantly (p < 0.05). In conclusion, addition of homologous SP to frozen-thawed stallion epididymal spermatozoa immediately improved motility in a dose-dependent manner regardless of semen quality of SP donor stallions. This might positively influence fertility when SP is added before insemination. Moreover, there seems to be a threshold level of SP concentration for optimal improvement of sperm motility. © 2015 American Society of Andrology and European Academy of Andrology.

Study on preferred crystal orientations of Mg-Zr-O composite protective layer in AC-PDP

NASA Astrophysics Data System (ADS)

Bingang, G.; Chunliang, L.; Zhongxiao, S.; Liu, L.; Yufeng, F.; Xing, X.; Duowang, F.

2006-11-01

In order to study the preferred crystal orientations of Mg-Zr-O composite protective layers in PDP, Mg-Zr-O composite protective layers were deposited by Electron-beam Evaporator using (MgO+ZrO{2}) powder mixture as evaporation source material. X-ray diffractometer (XRD) was used to determine preferred crystal orientations of Mg-Zr-O composite protective layers, surface morphologies of films were analyzed by FESEM and voltage characteristics were examined in a testing macroscopic discharge cell of AC-PDP. On the basis of experimental analysis, the influence of oxide addition and deposition conditions on preferred orientations of Mg-Zr-O composite protective layers were investigated. The results showed that the preferred orientations of Mg-Zr-O films were determined by lattice distortion of MgO crystal. The deposition conditions have great effects on the preferred orientations of Mg-Zr-O films. The preferred orientations affect voltage characteristics through affecting surface morphology of Mg-Zr-O films. A small amount of Zr solution in MgO can decrease firing voltage compared with using pure MgO film. Firing voltage is closely related with the [ ZrO{2}/(MgO+ZrO{2})] ratio of evaporation source materials.

Lowering of the firing voltage and reducing of the discharge delay time in alternating current plasma display panels by a discontinuous spin-coated LaB{sub 6} film on the MgO protective layer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deng, Jiang, E-mail: dj78291@163.com; Zeng, Baoqing; Zhongshan Institute, University of Electronic Science and Technology of China, 528402 zhongshan

2014-03-15

A spin coated LaB{sub 6} discontinuous film is covered on MgO protective layer to improve the discharge performance of alternating current plasma display panels. Under the premise of high transmittance of more than 90%, a very small amount of polycrystal LaB{sub 6} powders added in an organic solvent are chosen as the coating solution. The discharge characteristics results show that with 250 torr 5% Xe-Ne pressure, the firing voltage and discharge delay time of the test panel with LaB{sub 6}/MgO double protective layer are decreased by 13.4% and 36.5%, respectively, compared with that of conventional MgO protective layer, likely owingmore » to the low work function of LaB{sub 6.} Furthermore, the aging time of the proposed structure is comparable to that of pure MgO protective layer. Therefore, it will not increase the production costs and is highly suitable to be applied for alternating current plasma display panels with low electrical power consumption.« less

Reflection and Transmission of P-Waves in an Intermediate Layer Lying Between Two Semi-infinite Media

NASA Astrophysics Data System (ADS)

Singh, Pooja; Chattopadhyay, Amares; Srivastava, Akanksha; Singh, Abhishek Kumar

2018-05-01

With a motivation to gain physical insight of reflection as well as transmission phenomena in frozen (river/ocean) situation for example in Antarctica and other coldest place on Earth, the present article undertakes the analysis of reflection and transmission of a plane wave at the interfaces of layered structured comprised of a water layer of finite thickness sandwiched between an upper half-space constituted of ice and a lower isotropic elastic half-space, which may be useful in geophysical exploration in such conditions. A closed form expression of reflection/transmission coefficients of reflected and transmitted waves has been derived in terms of angles of incidence, propagation vector, displacement vector and elastic constants of the media. Expressions corresponding to the energy partition of various reflected and transmitted waves have also been established analytically. It has been remarkably shown that the law of conservation of energy holds good in the entire reflection and transmission phenomena for different angles of incidence. A numerical examples were performed so to graphically portray the analytical findings. Further the deduced results are validated with the pre-established classical results.

Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers.

PubMed

Macias, Maria I; Grande, Jesús; Moreno, Ana; Domínguez, Irene; Bornstein, Rafael; Flores, Ana I

2010-11-01

The objective of the study was to isolate and characterize a population of mesenchymal stem cells (MSCs) from human term placental membranes. We isolated an adherent cell population from extraembryonic membranes. Morphology, phenotype, growth characteristics, karyotype, and immunological and differentiation properties were analyzed. The isolated placental MSCs were from maternal origin and named as decidua-derived mesenchymal stem cells (DMSCs). DMSCs differentiated into derivatives of all germ layers. It is the first report about placental MSC differentiation into alveolar type II cells. Clonally expanded DMSCs differentiated into all embryonic layers, including pulmonary cells. DMSCs showed higher life span than placental cells from fetal origin and proliferated without genomic instability. The data suggest that DMSCs are true multipotent MSCs, distinguishing them from other placental MSCs. DMSCs could be safely used in the mother as a potential source of MSCs for pelvic floor dysfunctions and immunological diseases. Additionally, frozen DMSCs can be stored for both autologous and allogeneic tissue regeneration. Copyright © 2010 Mosby, Inc. All rights reserved.

Protecting nickel with graphene spin-filtering membranes: A single layer is enough

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martin, M.-B.; Dlubak, B.; Piquemal-Banci, M.

2015-07-06

We report on the demonstration of ferromagnetic spin injectors for spintronics which are protected against oxidation through passivation by a single layer of graphene. The graphene monolayer is directly grown by catalytic chemical vapor deposition on pre-patterned nickel electrodes. X-ray photoelectron spectroscopy reveals that even with its monoatomic thickness, monolayer graphene still efficiently protects spin sources against oxidation in ambient air. The resulting single layer passivated electrodes are integrated into spin valves and demonstrated to act as spin polarizers. Strikingly, the atom-thick graphene layer is shown to be sufficient to induce a characteristic spin filtering effect evidenced through the signmore » reversal of the measured magnetoresistance.« less

[Adaptability of sweet corn ears to a frozen process].

PubMed

Ramírez Matheus, Alejandra O; Martínez, Norelkys Maribel; de Bertorelli, Ligia O; De Venanzi, Frank

2004-12-01

The effects of frozen condition on the quality of three sweet corn ears (2038, 2010, 2004) and the pattern (Bonanza), were evaluated. Biometrics characteristics like ear size, ear diameter, row and kernel deep were measured as well as chemical and physical measurement in fresh and frozen states. The corn ears were frozen at -95 degrees C by 7 minutes. The yield and stability of the frozen ears were evaluated at 45 and 90 days of frozen storage (-18 degrees C). The average commercial yield as frozen corn ear for all the hybrids was 54.2%. The industry has a similar value range of 48% to 54%. The ear size average was 21.57 cm, row number was 15, ear diameter 45.54 mm and the kernel corn deep was 8.57 mm. All these measurements were found not different from commercial values found for the industry. All corn samples evaluated showed good stability despites the frozen processing and storage. Hybrid 2038 ranked higher in quality.

Outcomes Related to the Use of Frozen Plasma or Pooled Solvent/Detergent-Treated Plasma in Critically Ill Children.

PubMed

Camazine, Maraya N; Karam, Oliver; Colvin, Ryan; Leteurtre, Stephane; Demaret, Pierre; Tucci, Marisa; Muszynski, Jennifer A; Stanworth, Simon; Spinella, Philip C

2017-05-01

To determine if the use of fresh frozen plasma/frozen plasma 24 hours compared to solvent detergent plasma is associated with international normalized ratio reduction or ICU mortality in critically ill children. This is an a priori secondary analysis of a prospective, observational study. Study groups were defined as those transfused with either fresh frozen plasma/frozen plasma 24 hours or solvent detergent plasma. Outcomes were international normalized ratio reduction and ICU mortality. Multivariable logistic regression was used to determine independent associations. One hundred one PICUs in 21 countries. All critically ill children admitted to a participating unit were included if they received at least one plasma unit during six predefined 1-week (Monday to Friday) periods. All children were exclusively transfused with either fresh frozen plasma/frozen plasma 24 hours or solvent detergent plasma. None. There were 443 patients enrolled in the study. Twenty-four patients (5%) were excluded because no plasma type was recorded; the remaining 419 patients were analyzed. Fresh frozen plasma/frozen plasma 24 hours group included 357 patients, and the solvent detergent plasma group included 62 patients. The median (interquartile range) age and weight were 1 year (0.2-6.4) and 9.4 kg (4.0-21.1), respectively. There was no difference in reason for admission, severity of illness score, pretransfusion international normalized ratio, or lactate values; however, there was a difference in primary indication for plasma transfusion (p < 0.001). There was no difference in median (interquartile range) international normalized ratio reduction, between fresh frozen plasma/frozen plasma 24 hours and solvent detergent plasma study groups, -0.2 (-0.4 to 0) and -0.2 (-0.3 to 0), respectively (p = 0.80). ICU mortality was lower in the solvent detergent plasma versus fresh frozen plasma/frozen plasma 24 hours groups, 14.5% versus 29.1%%, respectively (p = 0.02). Upon adjusted analysis, solvent detergent plasma transfusion was independently associated with reduced ICU mortality (odds ratio, 0.40; 95% CI, 0.16-0.99; p = 0.05). Solvent detergent plasma use in critically ill children may be associated with improved survival. This hypothesis-generating data support a randomized controlled trial comparing solvent detergent plasma to fresh frozen plasma/frozen plasma 24 hours.

Mapping the Distribution of Traditional Iñupiat Ice Cellars in Barrow, Alaska

NASA Astrophysics Data System (ADS)

Klene, A. E.; Nyland, K.; Brown, J.; Shiklomanov, N. I.; Nelson, F. E.

2012-12-01

Historically, ice cellars excavated in permafrost have been essential to the Iñupiat residents of Barrow, Alaska, and remain so today. These traditional facilities, ranging in age from more than a century to newly excavated, allow secure, year-round frozen storage of subsistence harvests over long periods. Temperatures within the cellars are critical because bacteria can damage meat even at temperatures below the freezing point, and have traditionally been close to those of surrounding permafrost. Climatic change has been suspected of compromising and causing some ice cellars in Barrow to fill with water. Temperatures were monitored in five ice cellars, with little change observed over five years of observation, although sloughing was observed in one cellar. The lack of knowledge about the ice cellars as part of the local infrastructure led to a collaboration begun in 2012 with the North Slope Borough's Department of Planning and Community Services. Several meetings were held in August 2012 with local residents and stakeholders to assemble a GIS data layer of ice-cellar locations and conditions for use by researchers and by Borough representatives. Applications range from developing plans for snow plowing and construction to the protection of foodstuff quality and important cultural resources. Results from this collaboration will lead to improved understanding of the practical aspects of ice cellars use and maintenance in this urban Arctic environment.

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2010 CFR

2010-10-01

... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed foods...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 846.302-72 - Frozen processed foods.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Frozen processed foods... CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Clauses 846.302-72 Frozen processed foods. The contracting officer shall insert the clause at 852.246-72, Frozen processed foods, in solicitations and contracts for...

48 CFR 870.111-5 - Frozen processed food products.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed food... DEPARTMENT SUPPLEMENTARY REGULATIONS SPECIAL PROCUREMENT CONTROLS Controls 870.111-5 Frozen processed food products. (a) The following frozen processed food products must have a label complying with the Federal...

48 CFR 852.246-72 - Frozen processed foods.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Frozen processed foods. 852.246-72 Section 852.246-72 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS... Frozen processed foods. As prescribed in 846.302-72, insert the following clause: Frozen Processed Foods...

Effect of freezing on the rheological, chemical and colour properties of Serpa cheese.

PubMed

Alvarenga, Nuno; Canada, João; Sousa, Isabel

2011-02-01

The effect of freezing on the properties of a raw ewes'-milk semi-soft cheese (Serpa cheese) was studied using small amplitude oscillatory (SAOS) and texture measurements, colour and chemical parameters. The freezing was introduced at three different stages of the ripening process (28, 35 and 42 days), and the cheeses were maintained frozen for 12 months. Cheeses were submitted to a slow or fast freezing method, and to different storage temperatures: -10 and -20°C (three replicates for each set conditions). Chemical data showed that only the proteolysis indicators exhibited differences between frozen and non-frozen samples; frozen samples showed higher values of NPN than the non-frozen samples, indicating that the freezing process did not prevent the secondary proteolysis of cheese. Frozen samples showed a significantly (P<0·05) stronger structure than the non-frozen, as indicated by hardness. However, the differences between the frozen and non-frozen samples were not significantly for storage modulus (G' 1Hz) and loss tangent (tan δ 1Hz) (P>0·05). Freezing affected mainly colour parameters: frozen samples were more luminous, and more yellow-green. The results allowed us to conclude that the damages caused by freezing to cheese properties could be minimized if this type of storage is introduced at the end of ripening (42 d) using a freezing temperature of -20°C.

Incidence and prognostic factors for postoperative frozen shoulder after shoulder surgery: a prospective cohort study.

PubMed

Koorevaar, Rinco C T; Van't Riet, Esther; Ipskamp, Marcel; Bulstra, Sjoerd K

2017-03-01

Frozen shoulder is a potential complication after shoulder surgery. It is a clinical condition that is often associated with marked disability and can have a profound effect on the patient's quality of life. The incidence, etiology, pathology and prognostic factors of postoperative frozen shoulder after shoulder surgery are not known. The purpose of this explorative study was to determine the incidence of postoperative frozen shoulder after various operative shoulder procedures. A second aim was to identify prognostic factors for postoperative frozen shoulder after shoulder surgery. 505 consecutive patients undergoing elective shoulder surgery were included in this prospective cohort study. Follow-up was 6 months after surgery. A prediction model was developed to identify prognostic factors for postoperative frozen shoulder after shoulder surgery using the TRIPOD guidelines. We nominated five potential predictors: gender, diabetes mellitus, type of physiotherapy, arthroscopic surgery and DASH score. Frozen shoulder was identified in 11% of the patients after shoulder surgery and was more common in females (15%) than in males (8%). Frozen shoulder was encountered after all types of operative procedures. A prediction model based on four variables (diabetes mellitus, specialized shoulder physiotherapy, arthroscopic surgery and DASH score) discriminated reasonably well with an AUC of 0.712. Postoperative frozen shoulder is a serious complication after shoulder surgery, with an incidence of 11%. Four prognostic factors were identified for postoperative frozen shoulder: diabetes mellitus, arthroscopic surgery, specialized shoulder physiotherapy and DASH score. The combination of these four variables provided a prediction rule for postoperative frozen shoulder with reasonable fit. Level II, prospective cohort study.

Protective coatings for sensitive materials

DOEpatents

Egert, Charles M.

1997-01-01

An enhanced protective coating to prevent interaction between constituents of the environment and devices that can be damaged by those constituents. This coating is provided by applying a synergistic combination of diffusion barrier and physical barrier materials. These materials can be, for example, in the form of a plurality of layers of a diffusion barrier and a physical barrier, with these barrier layers being alternated. Further protection in certain instances is provided by including at least one layer of a getter material to actually react with one or more of the deleterious constituents. The coating is illustrated by using alternating layers of an organic coating (such as Parylene-C.TM.) as the diffusion barrier, and a metal coating (such as aluminum) as the physical barrier. For best results there needs to be more than one of at least one of the constituent layers.

Passivation coating for flexible substrate mirrors

DOEpatents

Tracy, C. Edwin; Benson, David K.

1990-01-01

A protective diffusion barrier for metalized mirror structures is provided by a layer or coating of silicon nitride which is a very dense, transparent, dielectric material that is impervious to water, alkali, and other impurities and corrosive substances that typically attack the metal layers of mirrors and cause degradation of the mirrors' reflectivity. The silicon nitride layer can be deposited on the substrate before metal deposition thereon to stabilize the metal/substrate interface, and it can be deposited over the metal to encapsulate it and protect the metal from corrosion or other degradation. Mirrors coated with silicon nitride according to this invention can also be used as front surface mirrors. Also, the silver or other reflective metal layer on mirrors comprising thin, lightweight, flexible substrates of metal or polymer sheets coated with glassy layers can be protected with silicon nitride according to this invention.

Spontaneous De-Icing Phenomena on Extremely Cold Surfaces

NASA Astrophysics Data System (ADS)

Song, Dong; Choi, Chang-Hwan

2017-11-01

Freezing of droplets on cold surfaces is universal phenomenon, while the mechanisms are still inadequately understood. Here we report spontaneous de-icing phenomena of an impacting droplet which occur on extreme cold surfaces. When a droplet impacts on cold surfaces lower than -80°, it takes more than two times longer for the droplet to freeze than the ones at -50°. Moreover, the frozen droplet below -80° breaks up into several large parts spontaneously in the end. When a droplet impacts on the extreme cold surfaces, evaporation and condensation occur immediately as the droplet approaches the substrate. A thick layer of frost forms between the droplet and substrate, decreasing the contact area of the droplet with substrate. It leads to impede the heat transfer and hence extends the freezing time significantly. On the extremely cold substrate, the droplet freezes from the center to the edge area, in contrast to a typical case freezing from the bottom to the top. This novel from-center-to-edge freezing process changes the internal tension of the frozen droplet and results in the instantaneous breakup and release eventually, which can be taken advantage of for effective deicing mechanisms.

Atomic Layer Deposited (ALD) coatings for future astronomical telescopes: recent developments

NASA Astrophysics Data System (ADS)

Moore, Christopher Samuel; Hennessy, John; Jewell, April D.; Nikzad, Shouleh; France, Kevin

2016-07-01

Atomic Layer Deposition (ALD) can create conformal, near stoichiometric and pinhole free transmissive metal fluoride coatings to protect reflective aluminum films. Spectral performance of astronomical mirror coatings strongly affect the science capabilities of astronomical satellite missions. We are utilizing ALD to create a transmissive overcoat to protect aluminum film mirrors from oxidation with the goal of achieving high reflectance (> 80%) from the UV ( 100 nm) to the IR ( 2,000 nm). This paper summarizes the recent developments of ALD aluminum fluoride (AlF3) coatings on Al. Reflectance measurements of aluminum mirrors protected by ALD AlF3 and future applications are discussed. These measurements demonstrate that Al + ALD AlF3, even with an interfacial oxide layer of a few nanometers, can provide higher reflectance than Al protected by traditional physical vapor deposited MgF2 without an oxide layer, below 115 nm.

76 FR 64001 - United States Standards for Grades of Frozen Okra

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-17

... to undertaking research and other work associated with revising the grade standards, AMS sought...-327] United States Standards for Grades of Frozen Okra AGENCY: Agricultural Marketing Service, USDA... (USDA) has revised the United States Standards for Grades of Frozen Okra. The grade standards for frozen...

Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation.

PubMed

Kumar, Pradeep; Kumar, Dharmendra; Sikka, P; Singh, P

2015-01-01

The variety of mammalian cells has been successfully cryopreserved by use of the silk protein sericin due to its strong free-radical-scavenging and potent antioxidant activity. The present study was conducted to examine the protective role of sericin on buffalo spermatozoa during cryopreservation. Semen of four breeding bulls was collected twice a week using artificial vagina technique. The ejaculates of four bulls were pooled, divided into five equal fractions, diluted with the extender supplemented with different concentrations of sericin (0, 0.25, 0.5, 1.5 and 2%) and then cryopreserved. Post-thawed motility was objectively assessed by computer assisted sperm analyzer. Sperm plasma membrane integrity was assessed by hypo-osmotic swelling test (HOST). Malondialdehyde (MDA) concentration, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in frozen-thawed extended seminal plasma by spectrophotometry. The extender supplemented with 0.25, 0.5 and 1% sericin resulted in the higher sperm motility and GPx acivity. Furthermore, plasma membrane integrity and SOD activity were found to be higher (P<0.05) in group supplemented with 0.25 and 0.5% sericin (P<0.05). The MDA concentration was found to be significantly lower (P<0.05) in 0.25 and 0.5% sericin treated groups than control and other treated groups. In conclusion, the supplementation of 0.25-0.5% sericin in semen extender improves frozen-thawed semen quality through protecting sperm from oxidative stress. Copyright © 2014 Elsevier B.V. All rights reserved.

Quality of Bovine Chilled or Frozen-Thawed Semen after Addition of Omega-3 Fatty Acids Supplementation to Extender

PubMed Central

Abavisani, Abbas; Arshami, Javad; Naserian, Abbas Ali; Sheikholeslami Kandelousi, Mohammad Ali; Azizzadeh, Mohammad

2013-01-01

Background: This study was conducted to evaluate the potential protective effects of omega-3 poly unsaturated fatty acids (Ω-3 PUFAs) on bovine sperm quality in response to cooling and cryopreservation. Materials and Methods: In this experimental study included ejaculates from five proven fertile bulls, allocated to the control and the four experimental groups. For group 1, polyethylene glycol (PEG) as a solvent was added alone to the extender, while for groups 2, 3 and 4, different concentration of omega-3 PUFAs (1, 2.5 and 5%, respectively) in combination with PEG were added to the semen extender. Motility [using computer aided sperm analysis (CASA)], viability and morphology of bovine sperm were investigated after 24 and 48 hours in both cold liquid storage and frozen-thawed conditions. Results: Our findings showed that PEG has some detrimental effects on sperm quality. Cooling as well as cryopreservation decreased significantly most of measured variables of sperm as compared to fresh semen, whereas the treatments did not improve sperm quality. Furthermore, levels of some variables were decreased significantly during treatments (p<0.05). Conclusion: Addition of Ω-3 PUFAs to semen extenders cannot be effectively introduced to conservation media as well as sperm membrane in order to protect spermatozoa in response to cooling and freezing. It can be suggested if Ω-3 PUFAs is supplemented to the diet of bulls in order to modify the fatty acid compositions of sperm, they might perform their preventive properties. PMID:24520481

Layer Protecting the Surface of Zirconium Used in Nuclear Reactors.

PubMed

Ashcheulov, Petr; Skoda, Radek; Skarohlíd, Jan; Taylor, Andrew; Fendrych, Frantisek; Kratochvílová, Irena

2016-01-01

Zirconium alloys have very useful properties for nuclear facilities applications having low absorption cross-section of thermal electrons, high ductility, hardness and corrosion resistance. However, there is also a significant disadvantage: it reacts with water steam and during this (oxidative) reaction it releases hydrogen gas, which partly diffuses into the alloy forming zirconium hydrides. A new strategy for surface protection of zirconium alloys against undesirable oxidation in nuclear reactors by polycrystalline diamond film has been patented- Czech patent 305059: Layer protecting the surface of zirconium alloys used in nuclear reactors and PCT patent: Layer for protecting surface of zirconium alloys (Patent Number: WO2015039636-A1). The zirconium alloy surface was covered by polycrystalline diamond layer grown in plasma enhanced chemical vapor deposition apparatus with linear antenna delivery system. Substantial progress in the description and understanding of the polycrystalline diamond/ zirconium alloys interface and material properties under standard and nuclear reactors conditions (irradiation, hot steam oxidation experiments and heating-quenching cycles) was made. In addition, process technology for the deposition of protective polycrystalline diamond films onto the surface of zirconium alloys was optimized. Zircaloy2 nuclear fuel pins were covered by 300 nm thick protective polycrystalline diamond layer (PCD) using plasma enhanced chemical vapor deposition apparatus with linear antenna delivery system. The polycrystalline diamond layer protects the zirconium alloy surface against undesirable oxidation and consolidates its chemical stability while preserving its functionality. PCD covered Zircaloy2 and standard Zircaloy2 pins were for 30 min. oxidized in 1100°C hot steam. Under these conditions α phase of zirconium changes to β phase (more opened for oxygen/hydrogen diffusion). PCD anticorrosion protection of Zircaloy nuclear fuel assemblies can significantly prolong lifetime of Zirconium alloy in nuclear reactors even above Zirconium phase transition temperatures. Even after ion beam irradiation (10 dpa, 3 MeV Fe(2+)) the diamond film still shows satisfactory structural integrity with both sp(3) and sp(2) carbon phases. Zircaloy2 under the carbon-based protective layer after hot steam oxidation test differed from the original Zircaloy2 material composition only very slightly, proving that the diamond coating increases the material resistance to high temperature oxidation. Zirconium alloys nuclear fuel pins' surfaces were covered by compact and homogeneous polycrystalline diamond layers consisting of sp(3) and sp(2) carbon phases with a high crystalline diamond content and low roughness. Diamond withstands very high temperatures, has excellent thermal conductivity and low chemical reactivity, it does not degrade over time and (important for the nuclear fuel cladding) being pure carbon, it has perfect neutron cross-section properties. Moreover, polycrystalline diamond layers consisting of crystalline (sp(3)) and amorphous (sp(2)) carbon phases could have suitable thermal expansion. Zirconium alloys coated with polycrystalline diamond film are protected against undesirable changes and processes. Further, the polycrystalline diamond layer prevents the reaction between the alloy surface and water vapor. During such reaction, water molecules dissociate and initiate formation of zirconium dioxide and hydrogen, accompanied by the release of large amount of heat. Thus the protective layer prevents the formation of hydrogen and the release of reaction heat. Few relevant patents to the topic have been reviewed and cited.

X-ray mirror prototype based on cold shaping of thin glass foils

NASA Astrophysics Data System (ADS)

Basso, Stefano; Civitani, Marta; Ghigo, Mauro; Hołyszko, Joanna; Pareschi, Giovanni; Salmaso, Bianca; Vecchi, Gabriele; Burwitz, Vadim; Pelliciari, Carlo; Hartner, Gisela D.; Breunig, Elias

2017-08-01

The Slumping Glass Optics technology for the fabrication of astronomical X-ray mirrors has been developed in recent years in USA and Europe. The process has been used for making the mirrors of the Nustar, mission. The process starts with very thin glass foils hot formed to copy the profile of replication moulds. At INAF - Osservatorio Astronomico di Brera a process based on cold shaping is being developed, based on an integration method involving the use of interconnecting ribs for making stacks. Each glass foil in the stack is shaped onto a very precise integration mould and the correct shape is frozen by means of glued ribs that act as spacers between one layer and the next one (the first layers being attached to a thick substrate). Therefore, the increasing availability of flexible glass foils with a thickness of a few tens of microns (driven by electronic market for ultra-thin displays) opens new possibilities for the fabrication of X-ray mirrors. This solution appears interesting especially for the fabrication of mirrors for hard X-rays (with energy > 10 keV) based on multilayer coatings, taking advantage from the intrinsic low roughness of the glass foils that should grant a low scattering level. The stress frozen on the glass due to the cold shaping is not negligible, but it is kept into account in the errors of the X-ray optics design. As an exercise, we have considered the requirements and specs of the FORCE hard Xray mission concept (being studied by JAXA) and we have designed the mirror modules assuming the cold slumping as a fabrication method. In the meantime, a prototype (representative of the FORCE mirror modules) is being design and integrated in order to demonstrate the feasibility and the capacity to reach good angular resolution.

Mask fabrication process

DOEpatents

Cardinale, Gregory F.

2000-01-01

A method for fabricating masks and reticles useful for projection lithography systems. An absorber layer is conventionally patterned using a pattern and etch process. Following the step of patterning, the entire surface of the remaining top patterning photoresist layer as well as that portion of an underlying protective photoresist layer where absorber material has been etched away is exposed to UV radiation. The UV-exposed regions of the protective photoresist layer and the top patterning photoresist layer are then removed by solution development, thereby eliminating the need for an oxygen plasma etch and strip and chances for damaging the surface of the substrate or coatings.

Displacements of Metallic Thermal Protection System Panels During Reentry

NASA Technical Reports Server (NTRS)

Daryabeigi, Kamran; Blosser, Max L.; Wurster, Kathryn E.

2006-01-01

Bowing of metallic thermal protection systems for reentry of a previously proposed single-stage-to-orbit reusable launch vehicle was studied. The outer layer of current metallic thermal protection system concepts typically consists of a honeycomb panel made of a high temperature nickel alloy. During portions of reentry when the thermal protection system is exposed to rapidly varying heating rates, a significant temperature gradient develops across the honeycomb panel thickness, resulting in bowing of the honeycomb panel. The deformations of the honeycomb panel increase the roughness of the outer mold line of the vehicle, which could possibly result in premature boundary layer transition, resulting in significantly higher downstream heating rates. The aerothermal loads and parameters for three locations on the centerline of the windward side of this vehicle were calculated using an engineering code. The transient temperature distributions through a metallic thermal protection system were obtained using 1-D finite volume thermal analysis, and the resulting displacements of the thermal protection system were calculated. The maximum deflection of the thermal protection system throughout the reentry trajectory was 6.4 mm. The maximum ratio of deflection to boundary layer thickness was 0.032. Based on previously developed distributed roughness correlations, it was concluded that these defections will not result in tripping the hypersonic boundary layer.

Effect of frozen storage on the structure and enzymatic activities of myofibrillar proteins of rabbit skeletal muscle.

PubMed

Kang, J O; Ito, T; Fukazawa, T

1983-01-01

The effect of frozen storage on the biochemical properties of myofibrils, and of their major constituents, actin and myosin, was investigated. Extractability of myofibrillar proteins increased slightly for 3 weeks during frozen storage of muscle, decreasing thereafter. The change in myofibrillar ATPase activity during frozen storage was consistent with that of a reconstituted acto-heavy meromyosin (HMM) complex prepared from frozen stored muscle at the same weight ratio of actin to myosin as in situ. However, myosin ATPase activity showed a different pattern of change when compared with myofibrillar ATPase activity. The maximum velocity of acto-HMM ATPase activity and the apparent dissociation constant of the acto-HMM complex decreased for 1 week during frozen storage, increasing thereafter, indicating that the affinity of actin for myosin was greatest in muscle which had been frozen for 1 week. Copyright © 1983. Published by Elsevier Ltd.

Deployable Debris Shields For Space Station

NASA Technical Reports Server (NTRS)

Christiansen, Eric L.; Cour-Palais, Burton G.; Crews, Jeanne

1993-01-01

Multilayer shields made of lightweight sheet materials deployed from proposed Space Station Freedom for additional protection against orbiting debris. Deployment mechanism attached at each location on exterior where extra protection needed. Equipment withdraws layer of material from storage in manner similar to unfurling sail or extending window shade. Number of layers deployed depends on required degree of protection, and could be as large as five.

Quantitative HAADF STEM of SiGe in presence of amorphous surface layers from FIB preparation.

PubMed

Grieb, Tim; Tewes, Moritz; Schowalter, Marco; Müller-Caspary, Knut; Krause, Florian F; Mehrtens, Thorsten; Hartmann, Jean-Michel; Rosenauer, Andreas

2018-01-01

The chemical composition of four Si 1-x Ge x layers grown on silicon was determined from quantitative scanning transmission electron microscopy (STEM). The chemical analysis was performed by a comparison of the high-angle annular dark field (HAADF) intensity with multislice simulations. It could be shown that amorphous surface layers originating from the preparation process by focused-ion beam (FIB) at 30 kV have a strong influence on the quantification: the local specimen thickness is overestimated by approximately a factor of two, and the germanium concentration is substantially underestimated. By means of simulations, the effect of amorphous surface layers on the HAADF intensity of crystalline silicon and germanium is investigated. Based on these simulations, a method is developed to analyze the experimental HAADF-STEM images by taking the influence of the amorphous layers into account which is done by a reduction of the intensities by multiplication with a constant factor. This suggested modified HAADF analysis gives germanium concentrations which are in agreement with the nominal values. The same TEM lamella was treated with low-voltage ion milling which removed the amorphous surface layers completely. The results from subsequent quantitative HAADF analyses are in agreement with the nominal concentrations which validates the applicability of the used frozen-lattice based multislice simulations to describe the HAADF scattering of Si 1-x Ge x in STEM. Copyright © 2017 Elsevier B.V. All rights reserved.

75 FR 22370 - Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's Republic of China...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

...-893, A-549-822, A-552-802] Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's... certain frozen warmwater shrimp from Brazil, Ecuador, India, the People's Republic of China, Thailand, and... Frozen and Canned Warmwater Shrimp From Ecuador, 69 FR 76913 (December 23, 2004) (Ecuador Final...

Abacus of Frozen Conflicts

DTIC Science & Technology

2010-06-01

NAVAL POSTGRADUATE SCHOOL MONTEREY, CALIFORNIA THESIS ABACUS OF FROZEN CONFLICTS by Reshad Karimov June 2010 Thesis...SUBTITLE Abacus of Frozen Conflicts 6. AUTHOR(S) Reshad Karimov 5. FUNDING NUMBERS 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Naval...PAGE INTENTIONALLY LEFT BLANK iii Approved for public release; distribution is unlimited ABACUS OF FROZEN CONFLICTS Reshad Karimov Research

76 FR 23277 - Certain Frozen Warmwater Shrimp From Brazil, India, the People's Republic of China, Thailand, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-26

... Fair Value: Certain Frozen and Canned Warmwater Shrimp From Brazil, 69 FR 76910 (December 23, 2004); Notice of Final Determination of Sales at Less Than Fair Value: Certain Frozen and Canned Warmwater... Fair Value and Negative Final Determination of Critical Circumstances: Certain Frozen and Canned...

21 CFR 161.176 - Frozen raw lightly breaded shrimp.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen raw lightly breaded shrimp. 161.176 Section 161.176 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Shellfish § 161.176 Frozen raw lightly breaded shrimp. Frozen raw lightly breaded shrimp complies with the...

21 CFR 161.176 - Frozen raw lightly breaded shrimp.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen raw lightly breaded shrimp. 161.176 Section 161.176 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Shellfish § 161.176 Frozen raw lightly breaded shrimp. Frozen raw lightly breaded shrimp complies with the...

21 CFR 146.137 - Frozen orange juice.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen orange juice. 146.137 Section 146.137 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135...

75 FR 69628 - Non-Frozen Apple Juice Concentrate From the People's Republic of China: Final Results of Sunset...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-15

... DEPARTMENT OF COMMERCE International Trade Administration [A-570-855] Non-Frozen Apple Juice... order on non-frozen apple juice concentrate from the People's Republic of China (``PRC''). Because the... June 5, 2000, the Department issued an antidumping duty order on certain non-frozen apple juice...

75 FR 29726 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Notice of Partial Rescission...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-27

... DEPARTMENT OF COMMERCE International Trade Administration [A-552-801] Certain Frozen Fish Fillets... antidumping duty order on certain frozen fish fillets from the Socialist Republic of Vietnam (``Vietnam''). See Notice of Antidumping Duty Order: Certain Frozen Fish Fillets From the Socialist Republic of...

76 FR 20626 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Extension of Time Limit for...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

... DEPARTMENT OF COMMERCE International Trade Administration [A-552-801] Certain Frozen Fish Fillets... and requests for revocation in part for certain frozen fish fillets from the Socialist Republic of... administrative reviews and requests for revocation in part for certain frozen fish fillets from the Socialist...

21 CFR 146.120 - Frozen concentrate for lemonade.

Code of Federal Regulations, 2011 CFR

2011-04-01

... lemonade is the frozen food prepared from one or both of the lemon juice ingredients specified in paragraph... percent by weight. (b) The lemon juice ingredients referred to in paragraph (a) of this section are: (1) Lemon juice or frozen lemon juice or a mixture of these. (2) Concentrated lemon juice or frozen...

78 FR 39708 - Certain Frozen Fish Fillets From the Socialist Republic of Vietnam: Final Results of Antidumping...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-02

... From the Socialist Republic of Vietnam: Final Results of Antidumping Duty New Shipper Reviews; 2011... frozen fish fillets (``frozen fish fillets'') from the Socialist Republic of Vietnam (``Vietnam'').\\1...'' section of this notice. \\1\\ See Certain Frozen Fish Fillets From the Socialist Republic of Vietnam...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 3 2011-01-01 2011-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 3 2012-01-01 2012-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 3 2014-01-01 2014-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

7 CFR 58.318 - Butter, frozen or plastic cream melting machines.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 3 2013-01-01 2013-01-01 false Butter, frozen or plastic cream melting machines. 58... Service 1 Equipment and Utensils § 58.318 Butter, frozen or plastic cream melting machines. Shavers, shredders or melting machines used for rapid melting of butter, frozen or plastic cream shall be of...

Fabrication of low-temperature solid oxide fuel cells with a nanothin protective layer by atomic layer deposition

PubMed Central

2013-01-01

Anode aluminum oxide-supported thin-film fuel cells having a sub-500-nm-thick bilayered electrolyte comprising a gadolinium-doped ceria (GDC) layer and an yttria-stabilized zirconia (YSZ) layer were fabricated and electrochemically characterized in order to investigate the effect of the YSZ protective layer. The highly dense and thin YSZ layer acted as a blockage against electron and oxygen permeation between the anode and GDC electrolyte. Dense GDC and YSZ thin films were fabricated using radio frequency sputtering and atomic layer deposition techniques, respectively. The resulting bilayered thin-film fuel cell generated a significantly higher open circuit voltage of approximately 1.07 V compared with a thin-film fuel cell with a single-layered GDC electrolyte (approximately 0.3 V). PMID:23342963

Calculation of wave-functions with frozen orbitals in mixed quantum mechanics/molecular mechanics methods. II. Application of the local basis equation.

PubMed

Ferenczy, György G

2013-04-05

The application of the local basis equation (Ferenczy and Adams, J. Chem. Phys. 2009, 130, 134108) in mixed quantum mechanics/molecular mechanics (QM/MM) and quantum mechanics/quantum mechanics (QM/QM) methods is investigated. This equation is suitable to derive local basis nonorthogonal orbitals that minimize the energy of the system and it exhibits good convergence properties in a self-consistent field solution. These features make the equation appropriate to be used in mixed QM/MM and QM/QM methods to optimize orbitals in the field of frozen localized orbitals connecting the subsystems. Calculations performed for several properties in divers systems show that the method is robust with various choices of the frozen orbitals and frontier atom properties. With appropriate basis set assignment, it gives results equivalent with those of a related approach [G. G. Ferenczy previous paper in this issue] using the Huzinaga equation. Thus, the local basis equation can be used in mixed QM/MM methods with small size quantum subsystems to calculate properties in good agreement with reference Hartree-Fock-Roothaan results. It is shown that bond charges are not necessary when the local basis equation is applied, although they are required for the self-consistent field solution of the Huzinaga equation based method. Conversely, the deformation of the wave-function near to the boundary is observed without bond charges and this has a significant effect on deprotonation energies but a less pronounced effect when the total charge of the system is conserved. The local basis equation can also be used to define a two layer quantum system with nonorthogonal localized orbitals surrounding the central delocalized quantum subsystem. Copyright © 2013 Wiley Periodicals, Inc.

Shock Chemistry of Organic Compounds Frozen in Ice Undergoing Impacts at 5 km s-1

NASA Astrophysics Data System (ADS)

Burchell, M. J.; Parnell, J. P.; Bowden, S.

2009-12-01

How complex organics developed is a key question in the study of the origin of life. One possibility is that existing molecules underwent shock driven synthesis into more complex forms. This could have occurred during high speed impacts onto planetary surfaces. Such impacts may also break apart existing complex molecules. Here we consider the case of impacts on icy bodies where existing organic molecules are frozen into the ice. As described in an earlier paper [1], a suite of 3 molecules were used; β,β carotene, stearic acid and anthracene. They have a range of origins (biological to abiological) and masses (178-536 daltons). They were mixed together and frozen in a water ice layer at 160 K. The ice targets were then impacted by stainless steel projectiles. The ejecta from the shots were collected at various angles of ejection and later analyzed by UV-VIS spectrometry and GC-MS. All the compounds were found in the ejecta although the concentrations varied significantly with angle of ejection [1]. In addition, some so far unidentified additional compounds were also found in the ejecta. Here the peak shock pressures in the experiments are estimated for the first time and the physical properties of the ejecta are discussed in more detail. We find for example that compared to impacts in pure water ice, the cratering efficiency in the organic rich ice is a factor of ˜4.5 times greater and the fraction of material removed as low angle spall is reduced. We also discuss the implications for application to space missions such as LCROSS to the Moon.

Frozen-Thawed Embryo Transfer Cycles Have a Lower Incidence of Ectopic Pregnancy Compared With Fresh Embryo Transfer Cycles.

PubMed

Zhang, Xinyu; Ma, Caihong; Wu, Zhangxin; Tao, Liyuan; Li, Rong; Liu, Ping; Qiao, Jie

2017-01-01

To evaluate the risk of ectopic pregnancy of embryo transfer. A retrospective cohort study on the incidence of ectopic pregnancy in fresh and frozen-thawed embryo transfer cycles from January 1 st , 2010, to January 1 st , 2015. Infertile women undergoing frozen-thawed transfer cycles or fresh transfer cycles. In-vitro fertilization, fresh embryo transfer, frozen-thawed embryo transfer, ectopic pregnancy. Ectopic pregnancy rate and clinical pregnancy rate. A total of 69 756 in vitro fertilization-embryo transfer cycles from 2010 to 2015 were analyzed, including 45 960 (65.9%) fresh and 23 796 (34.1%) frozen-thawed embryo transfer cycles. The clinical pregnancy rate per embryo transfer was slightly lower in fresh embryo transfer cycles compared with frozen-thawed embryo transfer cycles (40.8% vs 43.1%, P < .001). Frozen-thawed embryo transfer is associated with a lower incidence of ectopic pregnancy per clinical pregnancy, compared with fresh embryo transfers (odds ratio = 0.31; 95% confidence interval = 0.24-0.39). Female age and body mass index have no influence on ectopic pregnancy. In the frozen-thawed embryo transfer cycles, blastocyst transfer shows a significantly lower incidence of ectopic pregnancy (0.8% vs 1.8%, P = .002) in comparison with day 3 cleavage embryo transfer. The risk of ectopic pregnancy is lower in frozen-thawed embryo transfer cycles than fresh embryo transfer cycles, and blastocyst transfer could further decrease the ectopic pregnancy rate in frozen-thawed embryo transfer cycles.

Utility of intraoperative frozen sections in surgical decision making for acute invasive fungal rhinosinusitis.

PubMed

Papagiannopoulos, Peter; Lin, Diana Murro; Al-Khudari, Samer; Rajan, Kumar; Reddy, Swathi; Gattuso, Paulo; Tajudeen, Bobby; Batra, Pete S

2017-05-01

Acute invasive fungal rhinosinusitis (AIFRS) represents a fulminant, potentially fatal, disease process in immunocompromised patients. The diagnosis often rests on high index of clinical suspicion, with relative paucity of data on the diagnostic and therapeutic implications of intraoperative frozen sections. Retrospective review was performed for 18 cases undergoing endoscopic sinus surgery for AIFRS. Reliability of intraoperative frozen section diagnosis was evaluated for all patients using final pathology as the gold standard. A total of 66 frozen sections were performed. Diagnostic accuracy of frozen sections illustrated sensitivity of 72.7% (95% confidence interval [CI], 0.57 to 0.85), specificity of 100% (95% CI, 0.85 to 1.00), positive predictive value (PPV) of 100% (95% CI, 0.89 to 1.00), and negative predictive value (NPV) of 64.7% (95% CI, 0.46 to 0.80). There was no statistically significant difference in sensitivity of frozen sections in cases of Mucor and Aspergillus at 68.8%% and 76.2%, respectively (p = 0.61). This study represents the largest series assessing the diagnostic accuracy of frozen section analysis in AIFRS. Frozen section analysis is an effective tool for guiding intraoperative decision making in patients with AIFRS with a high PPV. A Low NPV underscores the importance of clinical suspicion and intraoperative decision making based on endoscopic findings when negative frozen section results are encountered. Further, frozen section analysis appears to be equally effective in detecting either Mucor or Aspergillus. © 2017 ARS-AAOA, LLC.

Rapid movement of frozen debris-lobes: implications for permafrost degradation and slope instability in the south-central Brooks Range, Alaska

USGS Publications Warehouse

Daanen, R.P.; Grosse, G.; Darrow, M.M.; Hamilton, T.D.; Jones, Benjamin M.

2012-01-01

We present the results of a reconnaissance investigation of unusual debris mass-movement features on permafrost slopes that pose a potential infrastructure hazard in the south-central Brooks Range, Alaska. For the purpose of this paper, we describe these features as frozen debris-lobes. We focus on the characterisation of frozen debris-lobes as indicators of various movement processes using ground-based surveys, remote sensing, field and laboratory measurements, and time-lapse observations of frozen debris-lobe systems along the Dalton Highway. Currently, some frozen debris-lobes exceed 100 m in width, 20 m in height and 1000 m in length. Our results indicate that frozen debris-lobes have responded to climate change by becoming increasingly active during the last decades, resulting in rapid downslope movement. Movement indicators observed in the field include toppling trees, slumps and scarps, detachment slides, striation marks on frozen sediment slabs, recently buried trees and other vegetation, mudflows, and large cracks in the lobe surface. The type and diversity of observed indicators suggest that the lobes likely consist of a frozen debris core, are subject to creep, and seasonally unfrozen surface sediment is transported in warm seasons by creep, slumping, viscous flow, blockfall and leaching of fines, and in cold seasons by creep and sliding of frozen sediment slabs. Ground-based measurements on one frozen debris-lobe over three years (2008–2010) revealed average movement rates of approximately 1 cm day−1, which is substantially larger than rates measured in historic aerial photography from the 1950s to 1980s. We discuss how climate change may further influence frozen debris-lobe dynamics, potentially accelerating their movement. We highlight the potential direct hazard that one of the studied frozen debris-lobes may pose in the coming years and decades to the nearby Trans Alaska Pipeline System and the Dalton Highway, the main artery for transportation between Interior Alaska and the North Slope.

Assessment of frozen storage duration effect on quality characteristics of various horse muscles.

PubMed

Seong, Pil Nam; Seo, Hyun Woo; Kim, Jin-Hyoung; Kang, Geun Ho; Cho, Soo-Hyun; Chae, Hyun Seok; Park, Beom Young; Van Ba, Hoa

2017-12-01

The study aimed at assessing the effects of frozen storage duration on quality characteristics, lipid oxidation and sensory quality of various horse muscles. Five representative muscles: longissimus dorsi (LD), gluteus medius (GM), semimembranosus (SM), biceps femoris (BF), and triceps brachii (TB) at 24 h post-mortem obtained from 28-mo-old Jeju female breed horses (n = 8) were used in the present investigation. The muscles were vacuum-packaged and frozen at -20°C for 120, 240, and 360 days. All the samples were analyzed for thawing and cooking losses, pH, Warner-Bratzler shear forces (WBSF), color traits, total volatile basic nitrogen (TVBN), thiobarbituric acid reactive substances (TBARS) and sensory traits. The muscle samples analyzed on day 0 of frozen storage (fresh, non-frozen) were used for comparison. Results revealed that thawing and cooking losses significantly (p<0.05) increased in all the muscles after 120 days and then remained unchanged up to 360 days of frozen storage. The TBARS and TVBN contents significantly increased as increasing frozen storage time up to 360 days (p<0.05). While, significant decreases in WBSF values were observed for all the muscles with increased frozen storage time (p<0.05). Frozen storage variously affected the color traits of the muscles for instance; the redness of LD, GM, and BF muscles showed a decreasing tendency during frozen storage while it was not changed in TB and SM muscles. Furthermore, the frozen storage did not produce detrimental effects on sensory quality as it did not cause flavor and juiciness defects whereas it partially improved the tenderness of all the muscles studied. Based on the results obtained from our work, it is concluded that frozen storage could be applied to increase the long-term shelf life of horsemeat while still retaining its sensory quality.

Synbiotic yogurt-ice cream produced via incorporation of microencapsulated lactobacillus acidophilus (la-5) and fructooligosaccharide.

PubMed

Ahmadi, Abbas; Milani, Elnaz; Madadlou, Ashkan; Mortazavi, Seyed Ali; Mokarram, Reza Rezaei; Salarbashi, Davoud

2014-08-01

Yogurt-ice cream is a nutritious product with a refreshing taste and durability profoundly longer than that of yogurt. The probiotic Lactobacillus acidophilus (La-5) cells either in free or encapsulated form were incorporated into yog-ice cream and their survivability were studied. Fructooligosaccharide (FOS) as a prebiotic compound at three levels (0, 4 & 8 % w/w) was added to yogurt-ice cream mix and its effects on some chemical properties, overrun and firmness of product were evaluated. The higher the incorporated FOS concentration, the lower were the pH value and higher the total solid content of treatments. FOS incorporation (8 %) significantly increased the overrun of treatments and reduced their firmness. The viable counts of free probiotics decreased from ~9.55 to ~7.3 log cfu/g after 60 days of frozen storage while that of encapsulated cells merely decreased less than 1 log cycle. Encapsulation with alginate microbeads protected the probiotic cells against injuries in the freezing stage as well as, during frozen storage.

Effects of different freezing methods on calcium enriched papaya (Carica papaya L.).

PubMed

Lovera, Nancy N; Ramallo, Laura; Salvadori, Viviana O

2018-06-01

The effect of calcium impregnation on drip loss, colour, mechanical properties, sensory perception and freezing time on frozen-thawed papaya was studied, evaluating different freezing methods: cryogenic, tunnel and household freezer freezing. Osmotic dehydration as pre-treatment was also evaluated. Freezing in liquid nitrogen was considered an inappropriate method for papaya preservation due to cracking. Calcium impregnation and osmotic dehydration increased tissue firmness and decreased freezing time (freezing time for fresh, calcium impregnated and osmo-dehydrated fruit was 23, 17 and 5 min in a tunnel and 118, 83 and 60 min in a household freezer, respectively). Calcium lactate was the most effective way to protect tissue's firmness before and after a freeze-thaw cycle (maximum stress values approx. 300-400% of the raw tissue for tunnel freezing and 260% for household freezer). Microstructure analysis showed better tissue integrity retention in papaya samples impregnated with calcium lactate than in those with calcium gluconate, after a freezing-thawing cycle, in agreement with the drip loss results. In spite of these results, consumers preferred frozen papaya without pre-treatment or impregnated with calcium gluconate.

Characterization of Textiles Used in Chefs' Uniforms for Protection Against Thermal Hazards Encountered in the Kitchen Environment.

PubMed

Zhang, Han; McQueen, Rachel H; Batcheller, Jane C; Ehnes, Briana L; Paskaluk, Stephen A

2015-10-01

Within the kitchen the potential for burn injuries arising from contact with hot surfaces, flames, hot liquid, and steam hazards is high. The chef's uniform can potentially offer some protection against such burns by providing a protective barrier between the skin and the thermal hazard, although the extent to which can provide some protection is unknown. The purpose of this study was to examine whether fabrics used in chefs' uniforms were able to provide some protection against thermal hazards encountered in the kitchen. Fabrics from chefs' jackets and aprons were selected. Flammability of single- and multiple-layered fabrics was measured. Effect of jacket type, apron and number of layers on hot surface, hot water, and steam exposure was also measured. Findings showed that all of the jacket and apron fabrics rapidly ignited when exposed to a flame. Thermal protection against hot surfaces increased as layers increased due to more insulation. Protection against steam and hot water improved with an impermeable apron in the system. For wet thermal hazards increasing the number of permeable layers can decrease the level of protection due to stored thermal energy. As the hands and arms are most at risk of burn injury increased insulation and water-impermeable barrier in the sleeves would improve thermal protection with minimal compromise to overall thermal comfort. © The Author 2015. Published by Oxford University Press on behalf of the British Occupational Hygiene Society.

Monitoring recharge in areas of seasonally frozen ground in the Columbia Plateau and Snake River Plain, Idaho, Oregon, and Washington

USGS Publications Warehouse

Mastin, Mark; Josberger, Edward

2014-01-01

Seasonally frozen ground occurs over approximately one‑third of the contiguous United States, causing increased winter runoff. Frozen ground generally rejects potential groundwater recharge. Nearly all recharge from precipitation in semi-arid regions such as the Columbia Plateau and the Snake River Plain in Idaho, Oregon, and Washington, occurs between October and March, when precipitation is most abundant and seasonally frozen ground is commonplace. The temporal and spatial distribution of frozen ground is expected to change as the climate warms. It is difficult to predict the distribution of frozen ground, however, because of the complex ways ground freezes and the way that snow cover thermally insulates soil, by keeping it frozen longer than it would be if it was not snow covered or, more commonly, keeping the soil thawed during freezing weather. A combination of satellite remote sensing and ground truth measurements was used with some success to investigate seasonally frozen ground at local to regional scales. The frozen-ground/snow-cover algorithm from the National Snow and Ice Data Center, combined with the 21-year record of passive microwave observations from the Special Sensor Microwave Imager onboard a Defense Meteorological Satellite Program satellite, provided a unique time series of frozen ground. Periodically repeating this methodology and analyzing for trends can be a means to monitor possible regional changes to frozen ground that could occur with a warming climate. The Precipitation-Runoff Modeling System watershed model constructed for the upper Crab Creek Basin in the Columbia Plateau and Reynolds Creek basin on the eastern side of the Snake River Plain simulated recharge and frozen ground for several future climate scenarios. Frozen ground was simulated with the Continuous Frozen Ground Index, which is influenced by air temperature and snow cover. Model simulation results showed a decreased occurrence of frozen ground that coincided with increased temperatures in the future climate scenarios. Snow cover decreased in the future climate scenarios coincident with the temperature increases. Although annual precipitation was greater in future climate scenarios, thereby increasing the amount of water available for recharge over current (baseline) simulations, actual evapotranspiration also increased and reduced the amount of water available for recharge over baseline simulations. The upper Crab Creek model shows no significant trend in the rates of recharge in future scenarios. In these scenarios, annual precipitation is greater than the baseline averages, offsetting the effects of greater evapotranspiration in future scenarios. In the Reynolds Creek Basin simulations, precipitation was held constant in future scenarios and recharge was reduced by 1.0 percent for simulations representing average conditions in 2040 and reduced by 4.3 percent for simulations representing average conditions in 2080. The focus of the results of future scenarios for the Reynolds Creek Basin was the spatial components of selected hydrologic variables for this 92 square mile mountainous basin with 3,600 feet of relief. Simulation results from the watershed model using the Continuous Frozen Ground Index provided a relative measure of change in frozen ground, but could not identify the within-soil processes that allow or reject available water to recharge aquifers. The model provided a means to estimate what might occur in the future under prescribed climate scenarios, but more detailed energy-balance models of frozen-ground hydrology are needed to accurately simulate recharge under seasonally frozen ground and provide a better understanding of how changes in climate may alter infiltration.

The S-layer Protein DR_2577 Binds Deinoxanthin and under Desiccation Conditions Protects against UV-Radiation in Deinococcus radiodurans

PubMed Central

Farci, Domenica; Slavov, Chavdar; Tramontano, Enzo; Piano, Dario

2016-01-01

Deinococcus radiodurans has the puzzling ability to withstand over a broad range of extreme conditions including high doses of ultraviolet radiation and deep desiccation. This bacterium is surrounded by a surface layer (S-layer) built of a regular repetition of several proteins, assembled to form a paracrystalline structure. Here we report that the deletion of a main constituent of this S-layer, the gene DR_2577, causes a decrease in the UVC resistance, especially in desiccated cells. Moreover, we show that the DR_2577 protein binds the carotenoid deinoxanthin, a strong protective antioxidant specific of this bacterium. A further spectroscopical characterization of the deinoxanthin-DR_2577 complex revealed features which could suggest a protective role of DR_2577. We propose that, especially under desiccation, the S-layer shields the bacterium from incident ultraviolet light and could behave as a first lane of defense against UV radiation. PMID:26909071

The S-layer Protein DR_2577 Binds Deinoxanthin and under Desiccation Conditions Protects against UV-Radiation in Deinococcus radiodurans.

PubMed

Farci, Domenica; Slavov, Chavdar; Tramontano, Enzo; Piano, Dario

2016-01-01

Deinococcus radiodurans has the puzzling ability to withstand over a broad range of extreme conditions including high doses of ultraviolet radiation and deep desiccation. This bacterium is surrounded by a surface layer (S-layer) built of a regular repetition of several proteins, assembled to form a paracrystalline structure. Here we report that the deletion of a main constituent of this S-layer, the gene DR_2577, causes a decrease in the UVC resistance, especially in desiccated cells. Moreover, we show that the DR_2577 protein binds the carotenoid deinoxanthin, a strong protective antioxidant specific of this bacterium. A further spectroscopical characterization of the deinoxanthin-DR_2577 complex revealed features which could suggest a protective role of DR_2577. We propose that, especially under desiccation, the S-layer shields the bacterium from incident ultraviolet light and could behave as a first lane of defense against UV radiation.

Protective coatings for sensitive materials

DOEpatents

Egert, C.M.

1997-08-05

An enhanced protective coating is disclosed to prevent interaction between constituents of the environment and devices that can be damaged by those constituents. This coating is provided by applying a synergistic combination of diffusion barrier and physical barrier materials. These materials can be, for example, in the form of a plurality of layers of a diffusion barrier and a physical barrier, with these barrier layers being alternated. Further protection in certain instances is provided by including at least one layer of a getter material to actually react with one or more of the deleterious constituents. The coating is illustrated by using alternating layers of an organic coating (such as Parylene-C{trademark}) as the diffusion barrier, and a metal coating (such as aluminum) as the physical barrier. For best results there needs to be more than one of at least one of the constituent layers. 4 figs.

Corrosion product layers on magnesium alloys AZ31 and AZ61: Surface chemistry and protective ability

NASA Astrophysics Data System (ADS)

Feliu, S.; Llorente, I.

2015-08-01

This paper studies the chemical composition of the corrosion product layers formed on magnesium alloys AZ31 and AZ61 following immersion in 0.6 M NaCl, with a view to better understanding their protective action. Relative differences in the chemical nature of the layers were quantified by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive analysis of X-ray (EDX) and low-angle X-ray diffraction (XRD). Corrosion behavior was investigated by Electrochemical Impedance Spectroscopy (EIS) and hydrogen evolution measurement. An inhibitive effect from the corrosion product layers was observed from EIS, principally in the case of AZ31, as confirmed by hydrogen evolution tests. A link was found between carbonate enrichment observed by XPS in the surface of the corrosion product layer, concomitant with the increase in the protective properties observed by EIS.

The role of organic soil layer on the fate of Siberian larch forest and near-surface permafrost under changing climate: A simulation study

NASA Astrophysics Data System (ADS)

SATO, H.; Iwahana, G.; Ohta, T.

2013-12-01

Siberian larch forest is the largest coniferous forest region in the world. In this vast region, larch often forms nearly pure stands, regenerated by recurrent fire. This region is characterized by a short and dry growing season; the annual mean precipitation for Yakutsk was only about 240 mm. To maintain forest ecosystem under such small precipitation, underlying permafrost and seasonal soil freezing-thawing-cycle have been supposed to play important roles; (1) frozen ground inhibits percolation of soil water into deep soil layers, and (2) excess soil water at the end of growing season can be carried over until the next growing season as ice, and larch trees can use the melt water. As a proof for this explanation, geographical distribution of Siberian larch region highly coincides with continuous and discontinuous permafrost zone. Recent observations and simulation studies suggests that existences of larch forest and permafrost in subsurface layer are co-dependent; permafrost maintains the larch forest by enhancing water use efficiency of trees, while larch forest maintains permafrost by inhibiting solar radiation and preventing heat exchanges between soil and atmosphere. Owing to such complexity and absence of enough ecosystem data available, current-generation Earth System Models significantly diverse in their prediction of structure and key ecosystem functions in Siberian larch forest under changing climate. Such uncertainty should in turn expand uncertainty over predictions of climate, because Siberian larch forest should have major role in the global carbon balance with its huge area and vast potential carbon pool within the biomass and soil, and changes in boreal forest albedo can have a considerable effect on Northern Hemisphere climate. In this study, we developed an integrated ecosystem model, which treats interactions between plant-dynamics and freeze-thaw cycles. This integrated model contains a dynamic global vegetation model SEIB-DGVM, which simulates plant and carbon dynamics. It also contains a one-dimensional land surface model NOAH 2.7.1, which simulates soil moisture (both liquid and frozen), soil temperature, snowpack depth and density, canopy water content, and the energy and water fluxes. This integrated model quantitatively reconstructs post-fire development of forest structure (i.e. LAI and biomass) and organic soil layer, which dampens heat exchanges between soil and atmosphere. With the post-fire development of LAI and the soil organic layer, the integrated model also quantitatively reconstructs changes in seasonal maximum of active layer depth. The integrated model is then driven by the IPCC A1B scenario of rising atmospheric CO2, and by climate changes during the twenty-first century resulting from the change in CO2. This simulation suggests that forecasted global warming would causes decay of Siberian larch ecosystem, but such responses could be delayed by "memory effect" of the soil organic layer for hundreds of years.

Thermal Protection Supplement for Reducing Interface Thermal Mismatch

NASA Technical Reports Server (NTRS)

Stewart, David A. (Inventor); Leiser, Daniel B. (Inventor)

2017-01-01

A thermal protection system that reduces a mismatch of thermal expansion coefficients CTE between a first material layer (CTE1) and a second material layer (CTE2) at a first layer-second layer interface. A portion of aluminum borosilicate (abs) or another suitable additive (add), whose CTE value, CTE(add), satisfies (CTE(add)-CTE1)(CTE(add)-CTE2)<0, is distributed with variable additive density,.rho.(z;add), in the first material layer and/or in the second material layer, with.rho.(z;add) near the materials interface being relatively high (alternatively, relatively low) and.rho.(z;add) in a region spaced apart from the interface being relatively low (alternatively, relatively high).

Modeling a two-layer flow system at the subarctic, subalpine tree line during snowmelt

NASA Astrophysics Data System (ADS)

Leenders, Erica E.; Woo, Ming-Ko

2002-10-01

In the subarctic it is common to encounter a two-layer flow system consisting of a porous organic cover overlying frozen or unfrozen mineral soils with much lower hydraulic conductivities. The "simple lumped reservoir parametric," or "semidistributed land-use-based runoff processes" (SLURP), model was adapted to simulate runoff generated by such a flow system from an upland shrub land to an open woodland downslope. A subalpine site in Wolf Creek, Yukon, Canada, was subdivided into two aggregated simulation areas (ASA), each being a unit characterized by a set of parameters. The model computes the vertical water balance and flow generation from several storages, and then routes the water out of the ASA. When applied to the 1999 snowmelt season, the model simulated the very low lateral flow and a large increase in storage in the mineral soil, as was observed in the field. The model was used to assess the sensitivity of the two-layer flow system under a range of temperature, snow cover, and frost conditions. Results show that within the range of possible climatic conditions, the hydrologic system is unlikely to yield significant runoff across the subalpine tree line, but if ground ice is abundant in the soil pores, percolation will be limited and fast flow from the surface layer is enhanced.

75 FR 61127 - Non-Frozen Apple Juice Concentrate from the People's Republic of China: Extension of Time Limit...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-04

... DEPARTMENT OF COMMERCE International Trade Administration [A-570-855] Non-Frozen Apple Juice... order on non-frozen apple juice concentrate from the People's Republic of China (``PRC'').\\1\\ This... currently due no later than October 28, 2010. \\1\\ See Certain Non-Frozen Apple Juice Concentrate from the...

75 FR 81564 - Certain Non-Frozen Apple Juice Concentrate From the People's Republic of China: Final Results of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-28

... DEPARTMENT OF COMMERCE International Trade Administration [A-570-855] Certain Non-Frozen Apple... order covering certain non-frozen apple juice concentrate from the People's Republic of China. See...: Certain Non-Frozen Apple Juice Concentrate From the People's Republic of China, 65 FR 35606 (June 5, 2000...

Solution-deposited F:SnO₂/TiO₂ as a base-stable protective layer and antireflective coating for microtextured buried-junction H₂-evolving Si photocathodes.

PubMed

Kast, Matthew G; Enman, Lisa J; Gurnon, Nicholas J; Nadarajah, Athavan; Boettcher, Shannon W

2014-12-24

Protecting Si photocathodes from corrosion is important for developing tandem water-splitting devices operating in basic media. We show that textured commercial Si-pn(+) photovoltaics protected by solution-processed semiconducting/conducting oxides (plausibly suitable for scalable manufacturing) and coupled to thin layers of Ir yield high-performance H2-evolving photocathodes in base. They also serve as excellent test structures to understand corrosion mechanisms and optimize interfacial electrical contacts between various functional layers. Solution-deposited TiO2 protects Si-pn(+) junctions from corrosion for ∼24 h in base, whereas junctions protected by F:SnO2 fail after only 1 h of electrochemical cycling. Interface layers consisting of Ti metal and/or the highly doped F:SnO2 between the Si and TiO2 reduce Si-emitter/oxide/catalyst contact resistance and thus increase fill factor and efficiency. Controlling the oxide thickness led to record photocurrents near 35 mA cm(-2) at 0 V vs RHE and photocathode efficiencies up to 10.9% in the best cells. Degradation, however, was not completely suppressed. We demonstrate that performance degrades by two mechanisms, (1) deposition of impurities onto the thin catalyst layers, even from high-purity base, and (2) catastrophic failure via pinholes in the oxide layers after several days of operation. These results provide insight into the design of hydrogen-evolving photoelectrodes in basic conditions, and highlight challenges.

Enhanced softgoods structures for spacesuit micrometeoroid/debris protective systems

NASA Technical Reports Server (NTRS)

Remington, Brian; Cadogan, David; Kosmo, Joseph

1992-01-01

A lightweight, flexible thermal micrometeoroid garment (TMG) design for enhanced space suit micrometeoroid/debris (M/D) protection is described. It will consist of an outer layer comprised of orthofabric, multilayers of aluminized Mylar, and a layer of silicone rubber loaded with micron sized particles of tungsten. The shield layers would fragment and/or vaporize the M/D projectile while the backup sheet would stop the resultant debris cloud.

Effect of Age and Bulls on Fresh Semen Quality and Frozen Semen Production of Holstein Bulls in Indonesia

NASA Astrophysics Data System (ADS)

Argiris, A.; Ondho, Y. S.; Santoso, S. I.; Kurnianto, E.

2018-02-01

Artificial Insemination is a compatible method of reproduction in an effort to increase dairy productivity. Artificial Insemination Center as a producer of frozen semen was required to maximize bulls in producing high quality frozen semen optimally. The purpose of this research was to determine effect of age and bulls on fresh semen quality and frozen semen production of Holstein bulls in Indonesia. The research was conducted at Lembang and Singosari AI Centers. The material used were 24.634 data of qualified fresh semen and frozen semen production from 81 Holstein Bulls aged 1-9 years that used as frozen semen producer in period of 2008 to 2016. The variables observed in this research were data of age of bulls, fresh semen volume (mL); sperm motility (%); mass movement; concentrations (million/mL) and frozen semen doses at each production at age of bulls. Nested design was applied to obtain and analyze data. Results showed that Age and bulls have significant effect (P<0,01) to volume, mass, motility, concentration and frozen semen production. Increasing the age of bulls resulted in increase semen volume until 7-year-old, while semen concentration decreased from 3 years old with increasing age. Frozen semen production, mass movement and motility shown the same relative value on 3-9 years old except on 1 to 2 years old had increase. Bulls would produce frozen semen optimally on 3-9 years old. Indeed, with knowledge of this factor, AI Centre might adapt management of AI bulls to improve semen production.

[Canopy vertical structure and understory plant regeneration of an evergreen broadleaved forest in Damingshan, Guangxi, China.

PubMed

Zhou, Xiao Guo; Wen, Yuan Guang; Zhu, Hong Guang; Wang, Lei; Li, Xiao Qiong

2017-02-01

In order to reveal the dynamics of canopy vertical structure and its effects on understory regeneration, we built 24 permanent plots (20 m×20 m) on the upslope, midslopeand downslope, respectively, in a typical evergreen broadleaved forest in Damingshan, Guangxi, China. We measured the crown area of each tree with diameter at breast height (DBH)≥1.0 cm, and surveyed the understory regeneration in growing season from 2009 to 2011. The results showed that the total canopy cover significantly increased from 54.0% in 2009 to 67.4% in 2011 after the frozen disaster in 2008. A significant difference existed in the cover and increment of different canopy layers. The canopy cover in the upper layers was markedly higher than that in the middle and lower layers. The increment of canopy coverage in the middle and lower layers was significantly higher than that in the upper layer. There were 55 regenerated woody plant species, and the dominant families and species of regenerated plants were in accord with those in the evergreen broadleaved forest. Biodiversity index of regenerated plants in the same slope position was significantly different among different years, and no significant difference was observed among different slope positions in the same year. The correlation between the coverage at different canopy layers and the species richness and abundance of regenerated plants was not significant. Total canopy cover and canopy coverage at the middle and lower layers were significantly negatively correlated with the Shannon index, Simpson index, and Pielou evenness index of the understory regenerated plants. It indicated that canopy coverage had a significant influence on the regeneration of understory, and the middle and lower layers had a stronger influence on the biodiversity of regenerated plants.

Combination of metallic impregnation and autoradiography of brain sections. A method for differentiation of proliferating glial cells in the brain of adult rats and mice.

PubMed

Korr, H

1978-12-29

After labeling with 14C-thymidine, frozen sections or paraffin sections of the brain of adult mice or rats were first stained by metallic impregnation and then coated with chrome alum gelatine and with an emulsion layer of about 10 micron. On the autoradiographs 14C-tracks are readily recognized above labelled astrocytes or oligodendrocytes, and these can be well discriminated, if the sections are processed by the silver carbonate method of Rio-Hortega. In contrast, no labelling is obtained, if the gold chloride sublimate method of Cajal is applied.

Engendering Long-Term Air and Light Stability of a TiO2-Supported Porphyrinic Dye via Atomic Layer Deposition.

PubMed

Hoffeditz, William L; Son, Ho-Jin; Pellin, Michael J; Farha, Omar K; Hupp, Joseph T

2016-12-21

Organic and porphyrin-based chromophores are prevalent in liquid-junction photovoltaic and photocatalytic solar-cell chemistry; however, their long-term air and light instability may limit their practicality in real world technologies. Here, we describe the protection of a zinc porphyrin dye, adsorbed on nanoparticulate TiO 2 , from air and light degradation by a protective coating of alumina grown with a previously developed post-treatment atomic layer deposition (ALD) technique. The protective Al 2 O 3 ALD layer is deposited using dimethylaluminum isopropoxide as an Al source; in contrast to the ubiquitous ALD precursor trimethylaluminum, dimethylaluminum isopropoxide does not degrade the zinc porphyrin dye, as confirmed by UV-vis measurements. The growth of this protective ALD layer around the dye can be monitored by an in-reactor quartz crystal microbalance (QCM). Furthermore, greater than 80% of porphyrin light absorption is retained over ∼1 month of exposure to air and light when the protective coating is present, whereas almost complete loss of porphyrin absorption is observed in less than 2 days in the absence of the ALD protective layer. Applying the Al 2 O 3 post-treatment technique to the TiO 2 -adsorbed dye allows the dye to remain in electronic contact with both the semiconductor surface and a surrounding electrolyte solution, the combination of which makes this technique promising for numerous other electrochemical photovoltaic and photocatalytic applications, especially those involving the dye-sensitized evolution of oxygen.

Impact of rockfalls on protection measures: an experimental approach

NASA Astrophysics Data System (ADS)

Yuan, J.; Li, Y.; Huang, R.; Pei, X.

2015-01-01

The determination of rockfall impact force is crucial in designing the protection measures. In the present study, laboratory tests are carried out by taking the weight and shape of the falling rock fragments, drop height, incident angle, platform on the slideway and cushion layer on the protection measures as factors to investigate their influences on the impact force. The test results indicate that the impact force is positively exponential to the weight of rockfall and the instantaneous impact velocity of the rockfall approaching the protection measures. The impact velocity is found to be dominated not only by the drop height but also by the shape of rockfall as well as the length of the platform on the slideway. A great drop height and/or a short platform produce a fast impact velocity. Spherical rockfalls experience a reater impact velocity than cubic and cylindrical ones. A layer of cushion on the protection measures may reduce the impact force to a greater extent. The reduction effects are dominated by the cushion material and the thickness of the cushion layer. The thicker the cushion layer, the greater the reduction effect and the less the impact force. The stiffer the buffer material, the less the buffering effect and the greater the impact force. The present study indicates that the current standard in China for designing protection measures may overestimate the impact force by taking no consideration for the rockfall shape, platform and cushion layer.

Cryo-irradiation as a terminal method for the sterilization of drug aqueous solutions.

PubMed

Maquille, Aubert; Habib Jiwan, Jean-Louis; Tilquin, Bernard

2008-05-01

The aim of this study is to evaluate the specificities of the irradiation of drugs in frozen aqueous solution. The structures of the degradation products were determined to gain insight into the radiolysis mechanisms occurring in frozen aqueous solutions. Metoclopramide hydrochloride and metoprolol tartrate were chosen as models. The frozen solutions were irradiated at dry ice temperature by high energy electrons at various doses. The drug purity (chemical potency) and the radiolysis products were quantified by HPLC-DAD. Characterization of the degradation products was performed by LC-APCI-MS-MS. The structures of the radiolysis products detected in irradiated frozen aqueous solutions were compared to those detected in solid-state and aqueous solutions (previous studies). For both metoclopramide and metoprolol, solute loss upon irradiation of frozen aqueous solutions was negligible. Five radiolysis products present in traces were identified in irradiated metoclopramide frozen solutions. Three of them were previously identified in solid-state irradiated metoclopramide crystals. The two others were formed following reactions with the hydroxyl radical (indirect effect). Only one fragmentation product was observed in irradiated metoprolol frozen solutions. For both drugs, radiosterilization of frozen solutions, even at high doses (25 kGy), was found to be possible.

Effects of enzymes to improve sensory quality of frozen dough bread and analysis on its mechanism.

PubMed

Wang, Xuan; Pei, Dudu; Teng, Yuefei; Liang, Jianfen

2018-01-01

Baking quality of frozen dough is negatively affected by dough weakening and by a reduction in both yeast viability and activity during freezing and frozen storage. The objective of this study was to investigate effects of different enzymes, such as α-amylase, xylanase, celluase, glucose oxidase, and lipase on the texture and sensory quality of bread after frozen storage, as well as on dough properties, in terms of fermentation characteristics, freezable water contents and microstructure. Except for α-amylase, other enzymes improved the bread sensory quality and got higher overall acceptability, especially xylanase. Dough fermentative behavior showed that the maximum heights of frozen dough were increased by 33.2, 19.7 and 7.4%, respectively with xylanase, cellulase and lipase. Cellulase lowered gas holding ability of dough. Thermodynamic properties indicated that addition of enzyme decreased the freezable water contents in frozen dough. Scanning electronic microscopy revealed that freezing and frozen storage disrupted dough gluten network causing separation of starch granules from the gluten matrix. Inclusion of cellulase, xylanase and lipase made the frozen dough having a more continuous gluten network and smoother surface, and glucose oxidase increased the stability of the gluten work.

Permafrost thaw and climate warming may decrease the CO2, carbon, and metal concentration in peat soil waters of the Western Siberia Lowland.

PubMed

Raudina, T V; Loiko, S V; Lim, A; Manasypov, R M; Shirokova, L S; Istigechev, G I; Kuzmina, D M; Kulizhsky, S P; Vorobyev, S N; Pokrovsky, O S

2018-09-01

Soil pore waters are a vital component of the ecosystem as they are efficient tracers of mineral weathering, plant litter leaching, and nutrient uptake by vegetation. In the permafrost environment, maximal hydraulic connectivity and element transport from soils to rivers and lakes occurs via supra-permafrost flow (i.e. water, gases, suspended matter, and solutes migration over the permafrost table). To assess possible consequences of permafrost thaw and climate warming on carbon and Green House gases (GHG) dynamics we used a "substituting space for time" approach in the largest frozen peatland of the world. We sampled stagnant supra-permafrost (active layer) waters in peat columns of western Siberia Lowland (WSL) across substantial gradients of climate (-4.0 to -9.1°C mean annual temperature, 360 to 600mm annual precipitation), active layer thickness (ALT) (>300 to 40cm), and permafrost coverage (sporadic, discontinuous and continuous). We analyzed CO 2 , CH 4 , dissolved carbon, and major and trace elements (TE) in 93 soil pit samples corresponding to several typical micro landscapes constituting the WSL territory (peat mounds, hollows, and permafrost subsidences and depressions). We expected a decrease in intensity of DOC and TE mobilization from soil and vegetation litter to the supra-permafrost water with increasing permafrost coverage, decreasing annual temperature and ALT along a latitudinal transect from 62.3°N to 67.4°N. However, a number of solutes (DOC, CO 2 , alkaline earth metals, Si, trivalent and tetravalent hydrolysates, and micronutrients (Mn, Co, Ni, Cu, V, Mo) exhibited a northward increasing trend with highest concentrations within the continuous permafrost zone. Within the "substituting space for time" climate change scenario and northward shift of the permafrost boundary, our results suggest that CO 2 , DOC, and many major and trace elements will decrease their concentration in soil supra-permafrost waters at the boundary between thaw and frozen layers. As a result, export of DOC and elements from peat soil to lakes and rivers of the WSL (and further to the Arctic Ocean) may decrease. Copyright © 2018 Elsevier B.V. All rights reserved.

Continuing Measurements of CO2 Crystals with a Hand-Held 35 GHz Radiometer

NASA Technical Reports Server (NTRS)

Foster, J.; Chang, A.; Hall, D.; Tait, A.; Wergin, W.; Erbe, E.

2000-01-01

In order to increase our knowledge of the Martian polar caps, an improved understanding of the behavior of both frozen H2O and CO2 in different parts of the electromagnetic spectrum is needed. The thermal microwave part of the spectrum has received relatively little attention compared to the visible and infrared wavelengths. A simple experiment to measure the brightness temperature of frozen CO2 was first performed in the winter of 1998 using a 35 GHz radiometer. in experiments performed during the winter of 1999 and 2000, passive microwave radiation emanating from within layers of manufactured CO2 (dry ice) crystals was again measured with a 35 GHz handheld radiometer. Both large (0.8 cm) and small (0.3 cm) cylindrical-shaped dry ice pellets, at a temperature of 197 K (-76 C), were measured. A 1 sq m plate of aluminum sheet metal was positioned beneath the dry ice so that microwave emissions from the underlying soil layers would be minimized. Non-absorbing foam was positioned around the sides of the plate in order to keep the dry ice in place and to assure that the incremental deposits were level. Thirty-five GHz measurements of this plate were made through the dry ice deposits in the following way. Layers of dry ice were built up and measurements were repeated for the increasing CO2 pack. First, 7 cm of large CO2 pellets were poured onto the sheet metal plate, then an additional 7 cm were added, and finally, 12 cm were added on top of the 14 cm base. Hand-held 35 GHz measurements were made each time the thickness of the deposit was increased. The same process was repeated for the smaller grain pellets. Furthermore, during the past winter, 35 GHz measurements were taken of a 25 kg (27 cm x 27 cm x 27 cm) solid cube Of CO2, which was cut in half and then re-measured. Additional information is contained in the original extended abstract.

Multilayer ultra-high-temperature ceramic coatings

DOEpatents

Loehman, Ronald E [Albuquerque, NM; Corral, Erica L [Tucson, AZ

2012-03-20

A coated carbon-carbon composite material with multiple ceramic layers to provide oxidation protection from ultra-high-temperatures, where if the carbon-carbon composite material is uninhibited with B.sub.4C particles, then the first layer on the composite material is selected from ZrB.sub.2 and HfB.sub.2, onto which is coated a layer of SiC coated and if the carbon-carbon composite material is inhibited with B.sub.4C particles, then protection can be achieved with a layer of SiC and a layer of either ZrB.sub.2 and HfB.sub.2 in any order.

Numerical analysis and comparison of three types of herringbone frame structure for highway subgrade slopes protection

NASA Astrophysics Data System (ADS)

Nie, Yihua; Tang, Saiqian; Xu, Yang; Mao, Kunli

2018-04-01

In order to obtain mechanical response distribution of herringbone frame structure for highway subgrade slopes protection and select the best structure type, 3D numerical models of three types herringbone frame structure were established and analyzed in finite element software ANSYS. Indoor physical model of soil slope protected by herringbone frame structure was built and mechanical response of the frame structure was measured by loading tests. Numerical results indicate slope foot is the stress most disadvantageous location. Comparative analysis shows that structure composed of mortar rubble base layer and precast concrete blocks paving layer is the best one for resisting deformation and structure with cement mortar base layer and precast concrete blocks paving layer is the best one for being of low stress.

Effect of sugar and acid on the acceptability of frozen yogurt to a student population.

PubMed

Guinard, J X; Little, C; Marty, C; Palchak, T R

1994-05-01

One hundred and forty-one college students tasted and rated on a nine-point hedonic scale their degree of liking for nine samples of vanilla frozen yogurt varying in sugar and lactic acid. Subjects were also asked to complete a questionnaire about consumption of frozen yogurt and other dairy products. Degree of liking differed significantly among samples, and the samples best liked were those with the lowest acidity, .23 to .29%, independent of sugar concentration. Degree of liking of frozen yogurt failed to correlate with dairy product consumption or hunger at the time of testing. No significant difference existed between male and female students for overall degree of liking of frozen yogurt or overall dairy product intake, yet the questionnaire revealed a significantly higher consumption of frozen yogurt among female students. The results of this study suggest that, for the student population tested, frozen yogurt should combine the sensory properties of ice cream (low acidity) with the nutritional properties of yogurt (low fat, active enzyme culture).

Intraoperative consultation of central nervous system lesions. Frozen section, cytology or both?

PubMed

Sharifabadi, Ali Haidari; Haeri, Hayedeh; Zeinalizadeh, Mehdi; Zargari, Neda; Razavi, Amirnader Emami; Shahbazi, Nargess; Tahvildari, Malahat; Azmoudeh-Ardalan, Farid

2016-03-01

Frozen section is the traditional method of assessing central nervous system (CNS) lesions intraoperatively. Our aim is to determine the diagnostic accuracy of frozen section and/or cytological evaluation of CNS lesions in our center. A total of 157 patients with CNS lesions underwent open surgical biopsy or excision in our center during a period of 2 years (2012-2013). All specimens were studied cytologically; of these specimens, 146 cases were also examined by frozen section. Cytology and frozen section slides were studied separately by two general pathologists who were blind to final diagnoses. The final diagnoses were based on permanent sections and IHC studies. The accuracy rates of frozen section analysis and cytological evaluation were 87% and 86%, respectively. If the two methods were considered together, the accuracy rate improved to about 95%. Cytological evaluation is an acceptable alternative to frozen section analysis and also a great supplement to the diagnosis of CNS lesions. Copyright © 2015 Elsevier GmbH. All rights reserved.

Metallic corrosion processes reactivation sustained by iron-reducing bacteria: Implication on long-term stability of protective layers

NASA Astrophysics Data System (ADS)

Esnault, L.; Jullien, M.; Mustin, C.; Bildstein, O.; Libert, M.

In deep geological environments foreseen for the disposal of radioactive waste, metallic containers will undergo anaerobic corrosion. In this context, the formation of corrosion products such as magnetite may reduce the rate of corrosion processes through the formation of a protective layer. This study aims at determining the direct impact of iron-reducing bacteria (IRB) activity on the stability of corrosion protective layers. Batch experiments investigating iron corrosion processes including the formation of secondary magnetite and its subsequent alteration in the presence of IRB show the bacteria ability to use structural Fe(III) for respiration which leads to the sustainment of a high corrosion rate. With the bio-reduction of corrosion products such as magnetite, and H 2 as electron donor, IRB promote the reactivation of corrosion processes in corrosive environments by altering the protective layer. This phenomenon could have a major impact on the long-term stability of metallic compounds involved in multi-barrier system for high-level radioactive waste containment.

MOFwich: Sandwiched Metal-Organic Framework-Containing Mixed Matrix Composites for Chemical Warfare Agent Removal.

PubMed

Peterson, Gregory W; Lu, Annie X; Hall, Morgan G; Browe, Matthew A; Tovar, Trenton; Epps, Thomas H

2018-02-28

This work describes a new strategy for fabricating mixed matrix composites containing layered metal-organic framework (MOF)/polymer films as functional barriers for chemical warfare agent protection. Through the use of mechanically robust polymers as the top and bottom encasing layers, a high-MOF-loading, high-performance-core layer can be sandwiched within. We term this multifunctional composite "MOFwich". We found that the use of elastomeric encasing layers enabled core layer reformation after breakage, an important feature for composites and membranes alike. The incorporation of MOFs into the core layer led to enhanced removal of chemical warfare agents while simultaneously promoting moisture vapor transport through the composite, showcasing the promise of these composites for protection applications.

Flexible Multi-Shock Shield

NASA Technical Reports Server (NTRS)

Christiansen, Eric L. (Inventor); Crews, Jeanne L. (Inventor)

2005-01-01

Flexible multi-shock shield system and method are disclosed for defending against hypervelocity particles. The flexible multi-shock shield system and method may include a number of flexible bumpers or shield layers spaced apart by one or more resilient support layers, all of which may be encapsulated in a protective cover. Fasteners associated with the protective cover allow the flexible multi-shock shield to be secured to the surface of a structure to be protected.

Corrosion resistant PEM fuel cell

DOEpatents

Li, Yang; Meng, Wen-Jin; Swathirajan, Swathy; Harris, Stephen Joel; Doll, Gary Lynn

2001-07-17

The present invention contemplates a PEM fuel cell having electrical contact elements (including bipolar plates/septums) comprising a titanium nitride coated light weight metal (e.g., Al or Ti) core, having a passivating, protective metal layer intermediate the core and the titanium nitride. The protective layer forms a barrier to further oxidation/corrosion when exposed to the fuel cell's operating environment. Stainless steels rich in CR, Ni, and Mo are particularly effective protective interlayers.

Corrosion resistant PEM fuel cell

DOEpatents

Li, Yang; Meng, Wen-Jin; Swathirajan, Swathy; Harris, Stephen Joel; Doll, Gary Lynn

2002-01-01

The present invention contemplates a PEM fuel cell having electrical contact elements (including bipolar plates/septums) comprising a titanium nitride coated light weight metal (e.g., Al or Ti) core, having a passivating, protective metal layer intermediate the core and the titanium nitride. The protective layer forms a barrier to further oxidation/corrosion when exposed to the fuel cell's operating environment. Stainless steels rich in CR, Ni, and Mo are particularly effective protective interlayers.

Corrosion resistant PEM fuel cell

DOEpatents

Li, Yang; Meng, Wen-Jin; Swathirajan, Swathy; Harris, Stephen J.; Doll, Gary L.

1997-01-01

The present invention contemplates a PEM fuel cell having electrical contact elements (including bipolar plates/septums) comprising a titanium nitride coated light weight metal (e.g., Al or Ti) core, having a passivating, protective metal layer intermediate the core and the titanium nitride. The protective layer forms a barrier to further oxidation/corrosion when exposed to the fuel cell's operating environment. Stainless steels rich in CR, Ni, and Mo are particularly effective protective interlayers.

21 CFR 101.95 - “Fresh,” “freshly frozen,” “fresh frozen,” “frozen fresh.”

Code of Federal Regulations, 2010 CFR

2010-04-01

... fresh.â 101.95 Section 101.95 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Descriptive Claims That Are Neither Nutrient Content Claims nor Health Claims § 101.95 “Fresh,” “freshly... frozen by a freezing system such as blast-freezing (sub-zero Fahrenheit temperature with fast moving air...

Recommendations for Improving the Food Service Operations at MCB Camp Pendleton, CA MCAS El Toro and MCAS(H) Santa Ana, CA, MCB Camp Lejeune, NC, and MCDEC, Quantico, VA

DTIC Science & Technology

1979-12-01

Green Beans Nicoise Egg Plant Parmesan or Zucchini Parmesan Club Spinach Dinner: Cheese Ravioli (frozen) rotate or Beef Ravioli (frozen) Cheese...Manicotti (frozen) rotate or Beef Manicotti (frozen) Meat Sauce (for ravioli and manicotti) Spaghetti w/ Meatballs Assorted Pizzas Baked Lasagna Veal Parmesan

Parylene-C passivation and effects on rectennas' wireless power transfer performance

NASA Astrophysics Data System (ADS)

Cooper, Camille; Eldridge, Keisharra; Kim, Min H.; Yoon, Hargsoon; Choi, Sang H.; Song, Kyo D.

2014-04-01

In this study, the effect of Parylene-C coated as a passivation layer on various rectennas is investigated in terms of their wireless power transfer performance. A passivation has been used for protection of rectenna circuits and their packaging in order for protection of the circuit elements and electrical insulation. Especially, wireless power receiving rectennas attached on sensors or on moving vehicles such as airship needs proper protection while they are exposed to harsh environment. In this research, a layer of Parylene-C thin film is used for passivation on rectennas and electromagnetic coupling by the coating is assessed by the measurement of receiving power levels. In this research, an electrochemical analysis method will also be introduced to measure the degree of water protection by a Parylene-C layer.

Silicon nitride protective coatings for silvered glass mirrors

DOEpatents

Tracy, C. Edwin; Benson, David K.

1988-01-01

A protective diffusion barrier for metalized mirror structures is provided by a layer or coating of silicon nitride which is a very dense, transparent, dielectric material that is impervious to water, alkali, and other impurities and corrosive substances that typically attack the metal layers of mirrors and cause degradation of the mirrors' reflectivity. The silicon nitride layer can be deposited on the substrate before metal deposition to stabilize the metal/substrate interface, and it can be deposited over the metal to encapsulate it and protect the metal from corrosion or other degradation. Mirrors coated with silicon nitride according to this invention can also be used as front surface mirrors.

Silicon nitride protective coatings for silvered glass mirrors

DOEpatents

Tracy, C.E.; Benson, D.K.

1984-07-20

A protective diffusion barrier for metalized mirror structures is provided by a layer or coating of silicon nitride which is a very dense, transparent, dielectric material that is impervious to water, alkali, and other impurities and corrosive substances that typically attack the metal layers of mirrors and cause degradation of the mirrors' reflectivity. The silicon nitride layer can be deposited on the substrate prior to metal deposition thereon to stabilize the metal/substrate interface, and it can be deposited over the metal to encapsulate it and protect the metal from corrosion or other degradation. Mirrors coated with silicon nitride according to this invention can also be used as front surface mirrors.

7 CFR 58.327 - Frozen cream.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Quality Specifications for Raw Material § 58.327 Frozen cream. To produce frozen cream eligible for official certification, the quality of...

7 CFR 58.327 - Frozen cream.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Specifications for Dairy Plants Approved for USDA Inspection and Grading Service 1 Quality Specifications for Raw Material § 58.327 Frozen cream. To produce frozen cream eligible for official certification, the quality of...

Frozen yogurt and ice cream were less healthy than yogurt, and adding toppings reduced their nutrition value: evidence from 1999-2014 National Health and Nutrition Examination Survey.

PubMed

An, Ruopeng; Jiang, Ning

2017-06-01

It was hypothesized that frozen yogurt and ice cream would be less healthy than yogurt. We examined daily energy and nutrient intake from yogurt, frozen yogurt, and ice cream among US adults. In-person 24-hour dietary recall data (n=6453) came from the 1999-2014 National Health and Nutrition Examination Survey. Regression analyses were performed to examine the differences in energy/nutrient intake from frozen yogurt and ice cream (with/without toppings) in comparison to yogurt. Approximately 5.3%, 0.9%, and 14.3% of US adults consumed yogurt, frozen yogurt, and ice cream on any given day, respectively. Among frozen yogurt and ice cream consumers, 29.7% and 14.8% added toppings to their consumption, respectively. Compared with yogurt, frozen yogurt consumption with and without toppings was associated with increased daily energy intake by 214.6 and 97.9kj, respectively; whereas ice cream consumption with and without toppings was associated with increased daily energy intake by 427.2 and 343.5kj, respectively. Compared with yogurt, frozen yogurt consumption was associated with a decreased intake of most vitamins/minerals under examination, but increased intake of sugar, total/saturated fat, cholesterol, fiber, and iron. Adding toppings to frozen yogurt further increased total and saturated fat intake. Compared with yogurt, ice cream consumption was associated with a decreased intake of multiple micronutrients, but increased intake of sugar, total/saturated fat, cholesterol, fiber, vitamins A and E, and iron. Adding toppings to ice cream further increased sugar intake. In conclusion, frozen yogurt and ice cream were less healthy than yogurt, and adding toppings made them even less desirable. Copyright © 2017 Elsevier Inc. All rights reserved.

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae.

PubMed

Teets, Nicholas M; Kawarasaki, Yuta; Lee, Richard E; Denlinger, David L

2011-03-01

In this study, we examined the effects of repeated cold exposure (RCE) on the survival, energy content and stress protein expression of larvae of the Antarctic midge, Belgica antarctica (Diptera: Chironomidae). Additionally, we compared results between larvae that were frozen at -5°C in the presence of water during RCE and those that were supercooled at -5°C in a dry environment. Although >95% of larvae survived a single 12 h bout of freezing at -5°C, after five cycles of RCE survival of frozen larvae dropped below 70%. Meanwhile, the survival of control and supercooled larvae was unchanged, remaining around 90% for the duration of the study. At the tissue level, frozen larvae had higher rates of cell mortality in the midgut than control and supercooled larvae. Furthermore, larvae that were frozen during RCE experienced a dramatic reduction in energy reserves; after five cycles, frozen larvae had 25% less lipid, 30% less glycogen and nearly 40% less trehalose than supercooled larvae. Finally, larvae that were frozen during RCE had higher expression of hsp70 than those that were supercooled, indicating a higher degree of protein damage in the frozen group. Results were similar between larvae that had accumulated 60 h of freezing at -5°C over five cycles of RCE and those that were frozen continuously for 60 h, suggesting that the total time spent frozen determines the physiological response. Our results suggest that it is preferable, both from a survival and energetic standpoint, for larvae to seek dry microhabitats where they can avoid inoculative freezing and remain unfrozen during RCE.

A simple temperature-based method to estimate heterogeneous frozen ground within a distributed watershed model

NASA Astrophysics Data System (ADS)

Follum, Michael L.; Niemann, Jeffrey D.; Parno, Julie T.; Downer, Charles W.

2018-05-01

Frozen ground can be important to flood production and is often heterogeneous within a watershed due to spatial variations in the available energy, insulation by snowpack and ground cover, and the thermal and moisture properties of the soil. The widely used continuous frozen ground index (CFGI) model is a degree-day approach and identifies frozen ground using a simple frost index, which varies mainly with elevation through an elevation-temperature relationship. Similarly, snow depth and its insulating effect are also estimated based on elevation. The objective of this paper is to develop a model for frozen ground that (1) captures the spatial variations of frozen ground within a watershed, (2) allows the frozen ground model to be incorporated into a variety of watershed models, and (3) allows application in data sparse environments. To do this, we modify the existing CFGI method within the gridded surface subsurface hydrologic analysis watershed model. Among the modifications, the snowpack and frost indices are simulated by replacing air temperature (a surrogate for the available energy) with a radiation-derived temperature that aims to better represent spatial variations in available energy. Ground cover is also included as an additional insulator of the soil. Furthermore, the modified Berggren equation, which accounts for soil thermal conductivity and soil moisture, is used to convert the frost index into frost depth. The modified CFGI model is tested by application at six test sites within the Sleepers River experimental watershed in Vermont. Compared to the CFGI model, the modified CFGI model more accurately captures the variations in frozen ground between the sites, inter-annual variations in frozen ground depths at a given site, and the occurrence of frozen ground.

The outcome of manipulation under general anesthesia for the management of frozen shoulder in patients with diabetes mellitus.

PubMed

Jenkins, Emily F; Thomas, William J C; Corcoran, John P; Kirubanandan, Ravisankar; Beynon, Celia R; Sayers, Adrian E; Woods, David A

2012-11-01

Frozen shoulder has a greater incidence, more severe course, and resistance to treatment in patients with diabetes mellitus compared with the general population. We hypothesized that diabetic patients with frozen shoulder undergoing treatment with manipulation under general anaesthesia (MUA) would have the same outcome as patients without diabetes. We retrospectively analyzed data collected during a 10-year period of referrals for frozen shoulder. In all cases, a standardized MUA protocol was followed once the diagnosis of frozen shoulder in the frozen phase was made; this included an early repeat MUA in individuals with recurrence. We compared outcomes for patients documented as having diabetes with a nondiabetic control group and assessed the effect of insulin dependence and frozen shoulder etiology within the diabetic group. Of a consecutive series of 315 frozen shoulders, 36 patients (39 shoulders) were included in the diabetic group, with 256 patients (274 shoulders) as controls. There was a significant improvement in range of movement and Oxford Shoulder Score (P all <.001), with no difference between diabetic and control groups at early or late follow-up (mean, 41 months). A repeat procedure was required in 36% of diabetic patients compared with 15% of control patients. Recurrence in the diabetic group was influenced by etiology (47% of primary vs 0% of secondary frozen shoulders) and insulin requirement (39% insulin-dependent vs. 31% non-insulin-dependent). We provide a strategy for the management of diabetic frozen shoulders using MUA and estimates of success and recurrence rates that may be useful when informing consent. Copyright © 2012 Journal of Shoulder and Elbow Surgery Board of Trustees. All rights reserved.

Capacitors Would Help Protect Against Hypervelocity Impacts

NASA Technical Reports Server (NTRS)

Edwards, David; Hubbs, Whitney; Hovater, Mary

2007-01-01

A proposal investigates alternatives to the present bumper method of protecting spacecraft against impacts of meteoroids and orbital debris. The proposed method is based on a British high-voltage-capacitance technique for protecting armored vehicles against shaped-charge warheads. A shield, according to the proposal, would include a bare metal outer layer separated by a gap from an inner metal layer covered with an electrically insulating material. The metal layers would constitute electrodes of a capacitor. A bias potential would be applied between the metal layers. A particle impinging at hypervelocity on the outer metal layer would break apart into a debris cloud that would penetrate the electrical insulation on the inner metal layer. The cloud would form a path along which electric current could flow between the metal layers, thereby causing the capacitor to discharge. With proper design, the discharge current would be large enough to vaporize the particles in the debris cloud to prevent penetration of the spacecraft. The shield design can be mass optimized to be competitive with existing bumper designs. Parametric studies were proposed to determine optimum correction between bias voltage, impacting particle velocity, gap space, and insulating material required to prevent spacecraft penetration.

Remote sensing of frozen lakes on the North Slope of Alaska

USGS Publications Warehouse

French, N.; Savage, S.; Shuchman, R.; Edson, R.; Payne, J.; Josberger, E.

2004-01-01

We used synthetic aperture radar (SAR) images from the ERS-2 remote sensing satellite to map the freeze condition of lakes on Alaska's North Slope, the geographic region to the north of the Brooks Range. An mage from March 1997, to coincide with the period of maximum freeze depth, was used for the frozen lake mapping. Emphasis was placed on distinguishing between lakes frozen to the lakebed and lakes with some portion unfrozen to the bed (a binary classification). The result of the analysis is a map identifying lakes as frozen to the lakebed and lakes not frozen to the lakebed. This analysis of one SAR image has shown the feasibility of a simple technique for mapping frozen lake condition for supporting decision making and understanding impacts of climate change on the North Slope.

An approach to successful freezing of demi-embryos derived from day-7 bovine embryos.

PubMed

Niemann, H; Brem, G; Sacher, B; Smidt, D; Kräusslich, H

1986-04-01

The developmental capacity of frozen/thawed bisected embryos (n = 33) derived from day-7 bovine embryos was investigated and compared to ordinary embryos after freezing and thawing (n = 28) and to freshly bisected embryos (n = 19). The freezing and thawing protocol was identical for ordinary and demi-embryos. The percentage of intact embryos classified as excellent, good, or poor after thawing was 92.9 and 96.3% for ordinary and demi-embryos, respectively. Pregnancy rates of 53.8 (8 15 ), 46.2 (6 13 ), and 47.5% (9 19 ) were obtained when frozen/thawed ordinary embryos and frozen/thawed demi-embryos classified as excellent or good and sealed with an additional zona pellucida from hatched pig blastocysts or freshly bisected embryos were transferred. One pair of identical twins resulted from the transfer of frozen/thawed demi-embryos sealed with an additional zona pellucida. Transfer of four frozen/thawed demi-embryos without an additional zona pellucida led to one pregnancy. In contrast, demi-embryos derived from frozen/thawed ordinary embryos (n = 8) as well as frozen/thawed demi-embryos classified as poor (n = 6) did not result in any pregnancies although two halves were transferred per recipient. It is concluded that sealing the punctured zona pellucida improves the developmental capacity of frozen/thawed demi-embryos derived from day-7 bovine embryos, and freezing demi-embryos is more efficient compared to the splitting of frozen/thawed ordinary embryos.

Application of waterproof breathable fabric in thermal protective clothing exposed to hot water and steam

NASA Astrophysics Data System (ADS)

Su, Y.; Li, R.; Song, G.; Li, J.

2017-10-01

A hot water and steam tester was used to examine thermal protective performance of waterproof and breathable fabric against hot water and steam hazards. Time to cause skin burn and thermal energy absorbed by skin during exposure and cooling phases was employed to characterize the effect of configuration, placing order and properties of waterproof and breathable fabric on the thermal protective performance. The difference of thermal protective performance due to hot water and steam hazards was discussed. The result showed that the configuration of waterproof and breathable fabric presented a significant effect on the thermal protective performance of single- and double-layer fabric system, while the difference between different configurations in steam hazard was greater than that in hot water hazard. The waterproof and breathable fabric as outer layer provided better protection than that as inner layer. Increasing thickness and moisture regain improved the thermal protective performance of fabric system. Additionally, the thermal energy absorbed by skin during the cooling phase was affected by configuration, thickness and moisture regain of fabric. The findings will provide technical data to improve performance of thermal protective clothing in hot water and steam hazards.

Ultrasonic measurements of thin zinc layers on concrete

NASA Astrophysics Data System (ADS)

Jansen, Henri; Brooks, Bill; Nguyen, Vinh; Koretsky, Milo

2008-05-01

In order to protect bridges at the coast from corrosion, a thin layer (approximately 0.5 mm) of zinc is sprayed on the concrete of the bridge. When this zinc layer is electrically connected to the reinforcing steel (rebar) and placed at a positive potential with respect to the rebar, oxidation is favored at the zinc layer and reduced at the rebar. The resulting protection of the rebar fails when the zinc layer delaminates from the concrete or when the zinc oxidation product layer becomes too thick. We have used ultrasonic detection to investigate the properties of the zinc layer. This method has been applied very successfully in the semiconductor industry. We present the details of the method and the expected response. Unfortunately, we are not able to measure changes in the zinc layer, because either the frequency we use (10-20 MHz) is too low, or scattering in the concrete is a dominant effect.