Sample records for protein engineering security

  1. Probing Enzyme-Surface Interactions via Protein Engineering and Single-Molecule Techniques

    DTIC Science & Technology

    2017-06-26

    SECURITY CLASSIFICATION OF: The overall objective of this research was to exploit protein engineering and fluorescence single-molecule methods to... Engineering and Single-Molecule Techniques The views, opinions and/or findings contained in this report are those of the author(s) and should not...Status: Technology Transfer: Report Date: 1 FINAL REPORT Project Title: Probing Enzyme-Surface Interactions via Protein Engineering and

  2. Integrated Controlling System and Unified Database for High Throughput Protein Crystallography Experiments

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gaponov, Yu.A.; Igarashi, N.; Hiraki, M.

    2004-05-12

    An integrated controlling system and a unified database for high throughput protein crystallography experiments have been developed. Main features of protein crystallography experiments (purification, crystallization, crystal harvesting, data collection, data processing) were integrated into the software under development. All information necessary to perform protein crystallography experiments is stored (except raw X-ray data that are stored in a central data server) in a MySQL relational database. The database contains four mutually linked hierarchical trees describing protein crystals, data collection of protein crystal and experimental data processing. A database editor was designed and developed. The editor supports basic database functions to view,more » create, modify and delete user records in the database. Two search engines were realized: direct search of necessary information in the database and object oriented search. The system is based on TCP/IP secure UNIX sockets with four predefined sending and receiving behaviors, which support communications between all connected servers and clients with remote control functions (creating and modifying data for experimental conditions, data acquisition, viewing experimental data, and performing data processing). Two secure login schemes were designed and developed: a direct method (using the developed Linux clients with secure connection) and an indirect method (using the secure SSL connection using secure X11 support from any operating system with X-terminal and SSH support). A part of the system has been implemented on a new MAD beam line, NW12, at the Photon Factory Advanced Ring for general user experiments.« less

  3. Information technology security system engineering methodology

    NASA Technical Reports Server (NTRS)

    Childs, D.

    2003-01-01

    A methodology is described for system engineering security into large information technology systems under development. The methodology is an integration of a risk management process and a generic system development life cycle process. The methodology is to be used by Security System Engineers to effectively engineer and integrate information technology security into a target system as it progresses through the development life cycle. The methodology can also be used to re-engineer security into a legacy system.

  4. Security Requirements Management in Software Product Line Engineering

    NASA Astrophysics Data System (ADS)

    Mellado, Daniel; Fernández-Medina, Eduardo; Piattini, Mario

    Security requirements engineering is both a central task and a critical success factor in product line development due to the complexity and extensive nature of product lines. However, most of the current product line practices in requirements engineering do not adequately address security requirements engineering. Therefore, in this chapter we will propose a security requirements engineering process (SREPPLine) driven by security standards and based on a security requirements decision model along with a security variability model to manage the variability of the artefacts related to security requirements. The aim of this approach is to deal with security requirements from the early stages of the product line development in a systematic way, in order to facilitate conformance with the most relevant security standards with regard to the management of security requirements, such as ISO/IEC 27001 and ISO/IEC 15408.

  5. Systems Security Engineering

    DTIC Science & Technology

    2010-08-22

    Commission (IEC). “Information technology — Security techniques — Code of practice for information security management ( ISO /IEC 27002 ...Information technology — Security techniques — Information security management systems —Requirements ( ISO /IEC 27002 ),”, “Information technology — Security...was a draft ISO standard on Systems and software engineering, Systems and software assurance [18]. Created by systems engineers for systems

  6. 40 CFR 1068.225 - What are the provisions for exempting engines/equipment for national security?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... engines/equipment for national security? 1068.225 Section 1068.225 Protection of Environment ENVIRONMENTAL... security? (a) An engine/equipment is exempt without a request if it will be used or owned by an agency of...) Manufacturers may request a national security exemption for engines/equipment not meeting the conditions of...

  7. 40 CFR 91.1008 - National security exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1008 National security exemption. (a)(1) Any marine SI engine, otherwise subject to this part... request a national security exemption for any marine SI engine, otherwise subject to this part, which does...

  8. DNA Nanotechnology-Enabled Interfacial Engineering for Biosensor Development.

    PubMed

    Ye, Dekai; Zuo, Xiaolei; Fan, Chunhai

    2018-06-12

    Biosensors represent biomimetic analytical tools for addressing increasing needs in medical diagnosis, environmental monitoring, security, and biodefense. Nevertheless, widespread real-world applications of biosensors remain challenging due to limitations of performance, including sensitivity, specificity, speed, and reproducibility. In this review, we present a DNA nanotechnology-enabled interfacial engineering approach for improving the performance of biosensors. We first introduce the main challenges of the biosensing interfaces, especially under the context of controlling the DNA interfacial assembly. We then summarize recent progress in DNA nanotechnology and efforts to harness DNA nanostructures to engineer various biological interfaces, with a particular focus on the use of framework nucleic acids. We also discuss the implementation of biosensors to detect physiologically relevant nucleic acids, proteins, small molecules, ions, and other biomarkers. This review highlights promising applications of DNA nanotechnology in interfacial engineering for biosensors and related areas.

  9. Designing Light-Activated Charge-Separating Proteins with a Naphthoquinone Amino Acid

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lichtenstein, Bruce R.; Bialas, Chris; Cerda, José F.

    2015-09-14

    The first principles design of manmade redox-protein maquettes is used to clarify the physical/chemical engineering supporting the mechanisms of natural enzymes with a view to recapitulate and surpass natural performance. Herein, we use intein-based protein semisynthesis to pair a synthetic naphthoquinone amino acid (Naq) with histidine-ligated photoactive metal–tetrapyrrole cofactors, creating a 100 μs photochemical charge separation unit akin to photosynthetic reaction centers. By using propargyl groups to protect the redox-active para-quinone during synthesis and assembly while permitting selective activation, we gain the ability to employ the quinone amino acid redox cofactor with the full set of natural amino acids inmore » protein design. Direct anchoring of quinone to the protein backbone permits secure and adaptable control of intraprotein electron-tunneling distances and rates.« less

  10. Providing security for automated process control systems at hydropower engineering facilities

    NASA Astrophysics Data System (ADS)

    Vasiliev, Y. S.; Zegzhda, P. D.; Zegzhda, D. P.

    2016-12-01

    This article suggests the concept of a cyberphysical system to manage computer security of automated process control systems at hydropower engineering facilities. According to the authors, this system consists of a set of information processing tools and computer-controlled physical devices. Examples of cyber attacks on power engineering facilities are provided, and a strategy of improving cybersecurity of hydropower engineering systems is suggested. The architecture of the multilevel protection of the automated process control system (APCS) of power engineering facilities is given, including security systems, control systems, access control, encryption, secure virtual private network of subsystems for monitoring and analysis of security events. The distinctive aspect of the approach is consideration of interrelations and cyber threats, arising when SCADA is integrated with the unified enterprise information system.

  11. Systems Security Engineering

    DTIC Science & Technology

    2010-08-22

    practice for information security management ( ISO /IEC 27002 ),” “Information technology — Security techniques — Information security management...systems —Requirements ( ISO /IEC 27002 ),”, “Information technology — Security techniques — Information security risk management ( ISO /IEC 27005).” from...associated practice aids. Perhaps the most germane discovery from this effort was a draft ISO standard on Systems and software engineering, Systems and

  12. Integration of Biological Specificity with Solid-State Devices for Selective Chemical Sensing

    DTIC Science & Technology

    2016-01-29

    materials onto a single sensor chip. We demonstrate a path to combine a large number of DNA aptamers with nanoscale device arrays to achieve integrated...solid-state, sensor chips with specificity. 15. SUBJECT TERMS DNA sensors aptamers chemiresistors nanosensors LSER specificity vapor 16. SECURITY...and engineering. In particular, DNA and RNA aptamers are a class of man- made receptors with a high degree of specificity that rivals proteins. DNA

  13. Genetically engineering milk.

    PubMed

    Whitelaw, C Bruce A; Joshi, Akshay; Kumar, Satish; Lillico, Simon G; Proudfoot, Chris

    2016-02-01

    It has been thirty years since the first genetically engineered animal with altered milk composition was reported. During the intervening years, the world population has increased from 5bn to 7bn people. An increasing demand for protein in the human diet has followed this population expansion, putting huge stress on the food supply chain. Many solutions to the grand challenge of food security for all have been proposed and are currently under investigation and study. Amongst these, genetics still has an important role to play, aiming to continually enable the selection of livestock with enhanced traits. Part of the geneticist's tool box is the technology of genetic engineering. In this Invited Review, we indicate that this technology has come a long way, we focus on the genetic engineering of dairy animals and we argue that the new strategies for precision breeding demand proper evaluation as to how they could contribute to the essential increases in agricultural productivity our society must achieve.

  14. 40 CFR 1068.315 - What are the permanent exemptions for imported engines/equipment?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... security exemption. You may import an engine or piece of equipment under the national security exemption in... engine. (d) Extraordinary circumstances exemption. You may import a nonconforming engine or piece of.... You may import a nonconforming engine or piece of equipment if we grant hardship relief for a small...

  15. 77 FR 4853 - In the Matter of Airbee Wireless, Inc., Axial Vector Engine Corp. (n/k/a Avec Corporation), and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-31

    ... Vector Engine Corp. (n/k/a Avec Corporation), and Exploration Drilling International, Inc.; Order of... securities of Axial Vector Engine Corp. (n/k/a Avec Corporation) because it has not filed any periodic...(k) of the Securities Exchange Act of 1934, that trading in the securities of the above-listed...

  16. Nuclear Security Education Program at the Pennsylvania State University

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Uenlue, Kenan; The Pennsylvania State University, Department of Mechanical and Nuclear Engineering, University Park, PA 16802-2304; Jovanovic, Igor

    The availability of trained and qualified nuclear and radiation security experts worldwide has decreased as those with hands-on experience have retired while the demand for these experts and skills have increased. The U.S. Department of Energy's National Nuclear Security Administration's (NNSA) Global Threat Reduction Initiative (GTRI) has responded to the continued loss of technical and policy expertise amongst personnel and students in the security field by initiating the establishment of a Nuclear Security Education Initiative, in partnership with Pennsylvania State University (PSU), Texas A and M (TAMU), and Massachusetts Institute of Technology (MIT). This collaborative, multi-year initiative forms the basismore » of specific education programs designed to educate the next generation of personnel who plan on careers in the nonproliferation and security fields with both domestic and international focus. The three universities worked collaboratively to develop five core courses consistent with the GTRI mission, policies, and practices. These courses are the following: Global Nuclear Security Policies, Detectors and Source Technologies, Applications of Detectors/Sensors/Sources for Radiation Detection and Measurements Nuclear Security Laboratory, Threat Analysis and Assessment, and Design and Analysis of Security Systems for Nuclear and Radiological Facilities. The Pennsylvania State University (PSU) Nuclear Engineering Program is a leader in undergraduate and graduate-level nuclear engineering education in the USA. The PSU offers undergraduate and graduate programs in nuclear engineering. The PSU undergraduate program in nuclear engineering is the largest nuclear engineering programs in the USA. The PSU Radiation Science and Engineering Center (RSEC) facilities are being used for most of the nuclear security education program activities. Laboratory space and equipment was made available for this purpose. The RSEC facilities include the Penn State Breazeale Reactor (PSBR), gamma irradiation facilities (in-pool irradiator, dry irradiator, and hot cells), neutron beam laboratory, radiochemistry laboratories, and various radiation detection and measurement laboratories. A new nuclear security education laboratory was created with DOE NNSA- GTRI funds at RSEC. The nuclear security graduate level curriculum enables the PSU to educate and train future nuclear security experts, both within the United States as well as worldwide. The nuclear security education program at Penn State will grant a Master's degree in nuclear security starting fall 2015. The PSU developed two courses: Nuclear Security- Detector And Source Technologies and Nuclear Security- Applications of Detectors/Sensors/Sources for Radiation Detection and Measurements (Laboratory). Course descriptions and course topics of these courses are described briefly: - Nuclear Security - Detector and Source Technologies; - Nuclear Security - Applications of Detectors/Sensors/Sources for Radiation Detection and Measurements Laboratory.« less

  17. High-throughput proteomics : optical approaches.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Davidson, George S.

    2008-09-01

    Realistic cell models could greatly accelerate our ability to engineer biochemical pathways and the production of valuable organic products, which would be of great use in the development of biofuels, pharmaceuticals, and the crops for the next green revolution. However, this level of engineering will require a great deal more knowledge about the mechanisms of life than is currently available. In particular, we need to understand the interactome (which proteins interact) as it is situated in the three dimensional geometry of the cell (i.e., a situated interactome), and the regulation/dynamics of these interactions. Methods for optical proteomics have become availablemore » that allow the monitoring and even disruption/control of interacting proteins in living cells. Here, a range of these methods is reviewed with respect to their role in elucidating the interactome and the relevant spatial localizations. Development of these technologies and their integration into the core competencies of research organizations can position whole institutions and teams of researchers to lead in both the fundamental science and the engineering applications of cellular biology. That leadership could be particularly important with respect to problems of national urgency centered around security, biofuels, and healthcare.« less

  18. MTR STACK, TRA710, CONTEXTUAL VIEW, CAMERA FACING SOUTH. PERIMETER SECURITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    MTR STACK, TRA-710, CONTEXTUAL VIEW, CAMERA FACING SOUTH. PERIMETER SECURITY FENCE AND SECURITY LIGHTING IN VIEW AT LEFT. INL NEGATIVE NO. HD52-1-1. Mike Crane, Photographer, 5/2005 - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  19. 14 CFR 49.15 - Fees for recording.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... therein $5.00 (2) Conveyance, made for security purposes, of a specifically identified aircraft engine or propeller, or any assignment or amendment thereof, or supplement thereto, recorded under Subpart D— For each engine or propeller 5.00 (3) Conveyance, made for security purposes, of aircraft engines, propellers...

  20. 14 CFR 49.15 - Fees for recording.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... therein $5.00 (2) Conveyance, made for security purposes, of a specifically identified aircraft engine or propeller, or any assignment or amendment thereof, or supplement thereto, recorded under Subpart D— For each engine or propeller 5.00 (3) Conveyance, made for security purposes, of aircraft engines, propellers...

  1. 14 CFR 49.15 - Fees for recording.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... therein $5.00 (2) Conveyance, made for security purposes, of a specifically identified aircraft engine or propeller, or any assignment or amendment thereof, or supplement thereto, recorded under Subpart D— For each engine or propeller 5.00 (3) Conveyance, made for security purposes, of aircraft engines, propellers...

  2. 14 CFR 49.15 - Fees for recording.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... therein $5.00 (2) Conveyance, made for security purposes, of a specifically identified aircraft engine or propeller, or any assignment or amendment thereof, or supplement thereto, recorded under Subpart D— For each engine or propeller 5.00 (3) Conveyance, made for security purposes, of aircraft engines, propellers...

  3. 14 CFR 49.15 - Fees for recording.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... therein $5.00 (2) Conveyance, made for security purposes, of a specifically identified aircraft engine or propeller, or any assignment or amendment thereof, or supplement thereto, recorded under Subpart D— For each engine or propeller 5.00 (3) Conveyance, made for security purposes, of aircraft engines, propellers...

  4. Transport and homeostasis of potassium and phosphate: limiting factors for sustainable crop production.

    PubMed

    Luan, Mingda; Tang, Ren-Jie; Tang, Yumei; Tian, Wang; Hou, Congong; Zhao, Fugeng; Lan, Wenzhi; Luan, Sheng

    2017-06-01

    Potassium (K) and phosphate (Pi) are both macronutrients essential for plant growth and crop production, but the unrenewable resources of phosphorus rock and potash have become limiting factors for food security. One critical measure to help solve this problem is to improve nutrient use efficiency (NUE) in plants by understanding and engineering genetic networks for ion uptake, translocation, and storage. Plants have evolved multiple systems to adapt to various nutrient conditions for growth and production. Within the NUE networks, transport proteins and their regulators are the primary players for maintaining nutrient homeostasis and could be utilized to engineer high NUE traits in crop plants. A large number of publications have detailed K+ and Pi transport proteins in plants over the past three decades. Meanwhile, the discovery and validation of their regulatory mechanisms are fast-track topics for research. Here, we provide an overview of K+ and Pi transport proteins and their regulatory mechanisms, which participate in the uptake, translocation, storage, and recycling of these nutrients in plants. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  5. Meet EPA Engineer Steve Clark

    EPA Pesticide Factsheets

    Steve Clark is an environmental engineer in EPA’s National Homeland Security Research Center (NHSRC). His research focuses on water security, exploring ways to protect and decontaminate pipes and other water “infrastructure.”

  6. IDEF3 Formalization Report

    DTIC Science & Technology

    1991-10-01

    SUBJECT TERMS 15. NUMBER OF PAGES engineering management information systems method formalization 60 information engineering process modeling 16 PRICE...CODE information systems requirements definition methods knowlede acquisition methods systems engineering 17. SECURITY CLASSIFICATION ji. SECURITY... Management , Inc., Santa Monica, California. CORYNEN, G. C., 1975, A Mathematical Theory of Modeling and Simula- tion. Ph.D. Dissertation, Department

  7. Security engineering: systems engineering of security through the adaptation and application of risk management

    NASA Technical Reports Server (NTRS)

    Gilliam, David P.; Feather, Martin S.

    2004-01-01

    Information Technology (IT) Security Risk Management is a critical task in the organization, which must protect its resources and data against the loss of confidentiality, integrity, and availability. As systems become more complex and diverse, and more vulnerabilities are discovered while attacks from intrusions and malicious content increase, it is becoming increasingly difficult to manage IT security. This paper describes an approach to address IT security risk through risk management and mitigation in both the institution and in the project life cycle.

  8. Security Quality Requirements Engineering (SQUARE): Case Study Phase III

    DTIC Science & Technology

    2006-05-01

    Security Quality Requirements Engineering (SQUARE): Case Study Phase III Lydia Chung Frank Hung Eric Hough Don Ojoko-Adams Advisor...Engineering (SQUARE): Case Study Phase III CMU/SEI-2006-SR-003 Lydia Chung Frank Hung Eric Hough Don Ojoko-Adams Advisor Nancy R. Mead...1 1.1 The SQUARE Process ............................................................................... 1 1.2 Case Study Clients

  9. 9. Photocopy of engineering drawing. SECURITY UPGRADES, SLC17: PLANS, SECTIONS, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Photocopy of engineering drawing. SECURITY UPGRADES, SLC17: PLANS, SECTIONS, AND DETAILS, JANUARY 1993. - Cape Canaveral Air Station, Launch Complex 17, Facility 28425, East end of Lighthouse Road, Cape Canaveral, Brevard County, FL

  10. 8. Photocopy of engineering drawing. SECURITY UPGRADES, SLC17: ELEVATIONS AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Photocopy of engineering drawing. SECURITY UPGRADES, SLC17: ELEVATIONS AND BUILDING SECTION, JULY 1992. - Cape Canaveral Air Station, Launch Complex 17, Facility 28425, East end of Lighthouse Road, Cape Canaveral, Brevard County, FL

  11. Volpe engineers use biometrics to help ease border crush

    DOT National Transportation Integrated Search

    1997-01-01

    Using technology previously reserved for military and other high security applications, engineers from the Safety and Security Systems Division of the Volpe Center have developed a number of automated biometric systems to speed the processing of freq...

  12. Secure web book to store structural genomics research data.

    PubMed

    Manjasetty, Babu A; Höppner, Klaus; Mueller, Uwe; Heinemann, Udo

    2003-01-01

    Recently established collaborative structural genomics programs aim at significantly accelerating the crystal structure analysis of proteins. These large-scale projects require efficient data management systems to ensure seamless collaboration between different groups of scientists working towards the same goal. Within the Berlin-based Protein Structure Factory, the synchrotron X-ray data collection and the subsequent crystal structure analysis tasks are located at BESSY, a third-generation synchrotron source. To organize file-based communication and data transfer at the BESSY site of the Protein Structure Factory, we have developed the web-based BCLIMS, the BESSY Crystallography Laboratory Information Management System. BCLIMS is a relational data management system which is powered by MySQL as the database engine and Apache HTTP as the web server. The database interface routines are written in Python programing language. The software is freely available to academic users. Here we describe the storage, retrieval and manipulation of laboratory information, mainly pertaining to the synchrotron X-ray diffraction experiments and the subsequent protein structure analysis, using BCLIMS.

  13. Process Security in Chemical Engineering Education

    ERIC Educational Resources Information Center

    Piluso, Cristina; Uygun, Korkut; Huang, Yinlun; Lou, Helen H.

    2005-01-01

    The threats of terrorism have greatly alerted the chemical process industries to assure plant security at all levels: infrastructure-improvement-focused physical security, information-protection-focused cyber security, and design-and-operation-improvement-focused process security. While developing effective plant security methods and technologies…

  14. Design tools for complex dynamic security systems.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Byrne, Raymond Harry; Rigdon, James Brian; Rohrer, Brandon Robinson

    2007-01-01

    The development of tools for complex dynamic security systems is not a straight forward engineering task but, rather, a scientific task where discovery of new scientific principles and math is necessary. For years, scientists have observed complex behavior but have had difficulty understanding it. Prominent examples include: insect colony organization, the stock market, molecular interactions, fractals, and emergent behavior. Engineering such systems will be an even greater challenge. This report explores four tools for engineered complex dynamic security systems: Partially Observable Markov Decision Process, Percolation Theory, Graph Theory, and Exergy/Entropy Theory. Additionally, enabling hardware technology for next generation security systemsmore » are described: a 100 node wireless sensor network, unmanned ground vehicle and unmanned aerial vehicle.« less

  15. Cyber-Informed Engineering

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Anderson, Robert S.; Benjamin, Jacob; Wright, Virginia L.

    A continuing challenge for engineers who utilize digital systems is to understand the impact of cyber-attacks across the entire product and program lifecycle. This is a challenge due to the evolving nature of cyber threats that may impact the design, development, deployment, and operational phases of all systems. Cyber Informed Engineering is the process by which engineers are made aware of both how to use their engineering knowledge to positively impact the cyber security in the processes by which they architect and design components and the services and security of the components themselves.

  16. 40 CFR 94.908 - National security exemption.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Exclusion and Exemption Provisions § 94.908 National security exemption. (a)(1) Any marine engine, otherwise subject to this part, that is... armor, permanently affixed weaponry, specialized electronic warfare systems, unique stealth performance...

  17. 40 CFR 94.908 - National security exemption.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Exclusion and Exemption Provisions § 94.908 National security exemption. (a)(1) Any marine engine, otherwise subject to this part, that is... armor, permanently affixed weaponry, specialized electronic warfare systems, unique stealth performance...

  18. 40 CFR 94.908 - National security exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Exclusion and Exemption Provisions § 94.908 National security exemption. (a)(1) Any marine engine, otherwise subject to this part, that is... armor, permanently affixed weaponry, specialized electronic warfare systems, unique stealth performance...

  19. 40 CFR 94.908 - National security exemption.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Exclusion and Exemption Provisions § 94.908 National security exemption. (a)(1) Any marine engine, otherwise subject to this part, that is... armor, permanently affixed weaponry, specialized electronic warfare systems, unique stealth performance...

  20. 40 CFR 94.908 - National security exemption.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Exclusion and Exemption Provisions § 94.908 National security exemption. (a)(1) Any marine engine, otherwise subject to this part, that is... armor, permanently affixed weaponry, specialized electronic warfare systems, unique stealth performance...

  1. Apparatus, system and method for providing cryptographic key information with physically unclonable function circuitry

    DOEpatents

    Areno, Matthew

    2015-12-08

    Techniques and mechanisms for providing a value from physically unclonable function (PUF) circuitry for a cryptographic operation of a security module. In an embodiment, a cryptographic engine receives a value from PUF circuitry and based on the value, outputs a result of a cryptographic operation to a bus of the security module. The bus couples the cryptographic engine to control logic or interface logic of the security module. In another embodiment, the value is provided to the cryptographic engine from the PUF circuitry via a signal line which is distinct from the bus, where any exchange of the value by either of the cryptographic engine and the PUF circuitry is for communication of the first value independent of the bus.

  2. Design of the subject of quality engineering and security of the product of the degree in engineering in industrial design and development of product based in the methodology of the case

    NASA Astrophysics Data System (ADS)

    González, M. R.; Lambán, M. P.

    2012-04-01

    This paper presents the result of designing the subject Quality Engineering and Security of the Product, belonging to the Degree of Engineering in Industrial Design and Product Development, on the basis of the case methodology. Practical sessions of this subject are organized using the whole documents of the Quality System Management of the virtual company BeaLuc S.A.

  3. The study on network security based on software engineering

    NASA Astrophysics Data System (ADS)

    Jia, Shande; Ao, Qian

    2012-04-01

    Developing a SP is a sensitive task because the SP itself can lead to security weaknesses if it is not conform to the security properties. Hence, appropriate techniques are necessary to overcome such problems. These techniques must accompany the policy throughout its deployment phases. The main contribution of this paper is then, the proposition of three of these activities: validation, test and multi-SP conflict management. Our techniques are inspired by the well established techniques of the software engineering for which we have found some similarities with the security domain.

  4. Capturing security requirements for software systems.

    PubMed

    El-Hadary, Hassan; El-Kassas, Sherif

    2014-07-01

    Security is often an afterthought during software development. Realizing security early, especially in the requirement phase, is important so that security problems can be tackled early enough before going further in the process and avoid rework. A more effective approach for security requirement engineering is needed to provide a more systematic way for eliciting adequate security requirements. This paper proposes a methodology for security requirement elicitation based on problem frames. The methodology aims at early integration of security with software development. The main goal of the methodology is to assist developers elicit adequate security requirements in a more systematic way during the requirement engineering process. A security catalog, based on the problem frames, is constructed in order to help identifying security requirements with the aid of previous security knowledge. Abuse frames are used to model threats while security problem frames are used to model security requirements. We have made use of evaluation criteria to evaluate the resulting security requirements concentrating on conflicts identification among requirements. We have shown that more complete security requirements can be elicited by such methodology in addition to the assistance offered to developers to elicit security requirements in a more systematic way.

  5. Capturing security requirements for software systems

    PubMed Central

    El-Hadary, Hassan; El-Kassas, Sherif

    2014-01-01

    Security is often an afterthought during software development. Realizing security early, especially in the requirement phase, is important so that security problems can be tackled early enough before going further in the process and avoid rework. A more effective approach for security requirement engineering is needed to provide a more systematic way for eliciting adequate security requirements. This paper proposes a methodology for security requirement elicitation based on problem frames. The methodology aims at early integration of security with software development. The main goal of the methodology is to assist developers elicit adequate security requirements in a more systematic way during the requirement engineering process. A security catalog, based on the problem frames, is constructed in order to help identifying security requirements with the aid of previous security knowledge. Abuse frames are used to model threats while security problem frames are used to model security requirements. We have made use of evaluation criteria to evaluate the resulting security requirements concentrating on conflicts identification among requirements. We have shown that more complete security requirements can be elicited by such methodology in addition to the assistance offered to developers to elicit security requirements in a more systematic way. PMID:25685514

  6. Development of undergraduate nuclear security curriculum at College of Engineering, Universiti Tenaga Nasional

    NASA Astrophysics Data System (ADS)

    Hamid, Nasri A.; Mujaini, Madihah; Mohamed, Abdul Aziz

    2017-01-01

    The Center for Nuclear Energy (CNE), College of Engineering, Universiti Tenaga Nasional (UNITEN) has a great responsibility to undertake educational activities that promote developing human capital in the area of nuclear engineering and technology. Developing human capital in nuclear through education programs is necessary to support the implementation of nuclear power projects in Malaysia in the near future. In addition, the educational program must also meet the nuclear power industry needs and requirements. In developing a certain curriculum, the contents must comply with the university's Outcomes Based Education (OBE) philosophy. One of the important courses in the nuclear curriculum is in the area of nuclear security. Basically the nuclear security course covers the current issues of law, politics, military strategy, and technology with regard to weapons of mass destruction and related topics in international security, and review legal regulations and political relationship that determine the state of nuclear security at the moment. In addition, the course looks into all aspects of the nuclear safeguards, builds basic knowledge and understanding of nuclear non-proliferation, nuclear forensics and nuclear safeguards in general. The course also discusses tools used to combat nuclear proliferation such as treaties, institutions, multilateral arrangements and technology controls. In this paper, we elaborate the development of undergraduate nuclear security course at the College of Engineering, Universiti Tenaga Nasional. Since the course is categorized as mechanical engineering subject, it must be developed in tandem with the program educational objectives (PEO) of the Bachelor of Mechanical Engineering program. The course outcomes (CO) and transferrable skills are also identified. Furthermore, in aligning the CO with program outcomes (PO), the PO elements need to be emphasized through the CO-PO mapping. As such, all assessments and distribution of Bloom Taxonomy levels are assigned in accordance with the CO-PO mapping. Finally, the course has to fulfill the International Engineering Alliance (IEA) Graduate Attributes of the Washington Accord.

  7. SAFETY AND SECURITY BUILDING, TRA614. ELEVATIONS. SECTIONS. TWO ROOF LEVELS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SAFETY AND SECURITY BUILDING, TRA-614. ELEVATIONS. SECTIONS. TWO ROOF LEVELS. BLAW-KNOX 3150-814-2, 3/1950. INL INDEX NO. 531-0614-00-098-100703, REV. 6. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  8. SAFETY AND SECURITY BUILDING, TRA614. SIMPLIFIED FLOOR LAYOUT AND WEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SAFETY AND SECURITY BUILDING, TRA-614. SIMPLIFIED FLOOR LAYOUT AND WEST ELEVATION. BLAW-KNOX 3150-14-1, 1/1950. INL INDEX NO. 531-0614-00-098-100024, REV. 2. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  9. Systems Security Engineering Capability Maturity Model SSE-CMM Model Description Document

    DTIC Science & Technology

    1999-04-01

    management is the process of accessing and quantifying risk , and establishing an acceptable level of risk for the organization. Managing risk is an...Process of assessing and quantifying risk and establishing acceptable level of risk for the organization. [IEEE 13335-1:1996] Security Engineering

  10. 77 FR 1942 - Homeland Security Science and Technology Advisory Committee (HSSTAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-12

    ... developments in systems engineering, cyber- security, knowledge management and how best to leverage related... contribution to a diverse range of science and technology topic areas (including chemical, biological, and... technology capabilities and needs, and the latest thinking in systems engineering), and their depth of...

  11. Photonics engineering: snapshot applications in healthcare, homeland security, agriculture, and industry

    NASA Astrophysics Data System (ADS)

    Sumriddetchkajorn, Sarun

    2015-01-01

    Throughout my experience in photonics engineering, this article shows that photonics is indeed a key technology enabler for enhancing our competitiveness. In particular, I snapshot the achievements of NECTEC research teams in implementing devices and systems suitable for healthcare, homeland security, agriculture, and industry.

  12. Processing multilevel secure test and evaluation information

    NASA Astrophysics Data System (ADS)

    Hurlburt, George; Hildreth, Bradley; Acevedo, Teresa

    1994-07-01

    The Test and Evaluation Community Network (TECNET) is building a Multilevel Secure (MLS) system. This system features simultaneous access to classified and unclassified information and easy access through widely available communications channels. It provides the necessary separation of classification levels, assured through the use of trusted system design techniques, security assessments and evaluations. This system enables cleared T&E users to view and manipulate classified and unclassified information resources either using a single terminal interface or multiple windows in a graphical user interface. TECNET is in direct partnership with the National Security Agency (NSA) to develop and field the MLS TECNET capability in the near term. The centerpiece of this partnership is a state-of-the-art Concurrent Systems Security Engineering (CSSE) process. In developing the MLS TECNET capability, TECNET and NSA are providing members, with various expertise and diverse backgrounds, to participate in the CSSE process. The CSSE process is founded on the concepts of both Systems Engineering and Concurrent Engineering. Systems Engineering is an interdisciplinary approach to evolve and verify an integrated and life cycle balanced set of system product and process solutions that satisfy customer needs (ASD/ENS-MIL STD 499B 1992). Concurrent Engineering is design and development using the simultaneous, applied talents of a diverse group of people with the appropriate skills. Harnessing diverse talents to support CSSE requires active participation by team members in an environment that both respects and encourages diversity.

  13. Protein engineering and its applications in food industry.

    PubMed

    Kapoor, Swati; Rafiq, Aasima; Sharma, Savita

    2017-07-24

    Protein engineering is a young discipline that has been branched out from the field of genetic engineering. Protein engineering is based on the available knowledge about the proteins structure/function(s), tools/instruments, software, bioinformatics database, available cloned gene, knowledge about available protein, vectors, recombinant strains and other materials that could lead to change in the protein backbone. Protein produced properly from genetic engineering process means a protein that is able to fold correctly and to do particular function(s) efficiently even after being subjected to engineering practices. Protein is modified through its gene or chemically. However, modification of protein through gene is easier. There is no specific limitation of Protein Engineering tools; any technique that can lead to change the protein constituent of amino acid and result in the modification of protein structure/function is in the frame of Protein Engineering. Meanwhile, there are some common tools used to reach a specific target. More active industrial and pharmaceutical based proteins have been invented by the field of Protein Engineering to introduce new function as well as to change its interaction with surrounding environment. A variety of protein engineering applications have been reported in the literature. These applications range from biocatalysis for food and industry to environmental, medical and nanobiotechnology applications. Successful combinations of various protein engineering methods had led to successful results in food industries and have created a scope to maintain the quality of finished product after processing.

  14. 123. ARAI Substation (ARA726) plan, elevation, security fence details, and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    123. ARA-I Substation (ARA-726) plan, elevation, security fence details, and sections. Norman Engineering Company 961-area/SF-726-E-1. Date: January 1959. Ineel index code no. 068-0726-10-613-102778. - Idaho National Engineering Laboratory, Army Reactors Experimental Area, Scoville, Butte County, ID

  15. SAFETY AND SECURITY BUILDING, TRA614. FLOOR, ROOF, AND FOUNDATION PLANS. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SAFETY AND SECURITY BUILDING, TRA-614. FLOOR, ROOF, AND FOUNDATION PLANS. ROOM FUNCTIONS. DOOR AND ROOM FINISH SCHEDULE. BLAW-KNOX 3150-814-1, 3/1950. INL INDEX NO. 531-0614-00-098-100702, REV. 6. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  16. Choosing a Global Positioning System Device for Use in U.S. Army Corps of Engineers Regulatory Districts

    DTIC Science & Technology

    2017-12-01

    Information Systems Center of Expertise (RS/GIS CX) (CEERD-RZR), U.S. Army Engineer Research and Development Center, Cold Regions Research and...GIS Geographic Information Systems GPS Global Positioning System HH Handheld IWR U.S. Army Engineer Institute for Water Resources n/a Not...Applicable NAE U.S. Army New England Regulatory District RS/GIS Remote Sensing/Geographic Information Systems SD Secure Digital SDHC Secure Digital High

  17. About the Transportation Secure Data Center | Transportation Secure Data

    Science.gov Websites

    Center | NREL About the Transportation Secure Data Center About the Transportation Secure Data Center The Transportation Secure Data Center (TSDC) makes vital transportation data broadly available large, colorful map of the United States. NREL data experts and engineers analyze large sets of complex

  18. Micromotor-based lab-on-chip immunoassays

    NASA Astrophysics Data System (ADS)

    García, Miguel; Orozco, Jahir; Guix, Maria; Gao, Wei; Sattayasamitsathit, Sirilak; Escarpa, Alberto; Merkoçi, Arben; Wang, Joseph

    2013-01-01

    Here we describe the first example of using self-propelled antibody-functionalized synthetic catalytic microengines for capturing and transporting target proteins between the different reservoirs of a lab-on-a-chip (LOC) device. A new catalytic polymer/Ni/Pt microtube engine, containing carboxy moieties on its mixed poly(3,4-ethylenedioxythiophene) (PEDOT)/COOH-PEDOT polymeric outermost layer, is further functionalized with the antibody receptor to selectively recognize and capture the target protein. The new motor-based microchip immunoassay operations are carried out without any bulk fluid flow, replacing the common washing steps in antibody-based protein bioassays with the active transport of the captured protein throughout the different reservoirs, where each step of the immunoassay takes place. A first microchip format involving an `on-the-fly' double-antibody sandwich assay (DASA) is used for demonstrating the selective capture of the target protein, in the presence of excess of non-target proteins. A secondary antibody tagged with a polymeric-sphere tracer allows the direct visualization of the binding events. In a second approach the immuno-nanomotor captures and transports the microsphere-tagged antigen through a microchannel network. An anti-protein-A modified microengine is finally used to demonstrate the selective capture, transport and convenient label-free optical detection of a Staphylococcus aureus target bacteria (containing proteinA in its cell wall) in the presence of a large excess of non-target (Saccharomyces cerevisiae) cells. The resulting nanomotor-based microchip immunoassay offers considerable potential for diverse applications in clinical diagnostics, environmental and security monitoring fields.Here we describe the first example of using self-propelled antibody-functionalized synthetic catalytic microengines for capturing and transporting target proteins between the different reservoirs of a lab-on-a-chip (LOC) device. A new catalytic polymer/Ni/Pt microtube engine, containing carboxy moieties on its mixed poly(3,4-ethylenedioxythiophene) (PEDOT)/COOH-PEDOT polymeric outermost layer, is further functionalized with the antibody receptor to selectively recognize and capture the target protein. The new motor-based microchip immunoassay operations are carried out without any bulk fluid flow, replacing the common washing steps in antibody-based protein bioassays with the active transport of the captured protein throughout the different reservoirs, where each step of the immunoassay takes place. A first microchip format involving an `on-the-fly' double-antibody sandwich assay (DASA) is used for demonstrating the selective capture of the target protein, in the presence of excess of non-target proteins. A secondary antibody tagged with a polymeric-sphere tracer allows the direct visualization of the binding events. In a second approach the immuno-nanomotor captures and transports the microsphere-tagged antigen through a microchannel network. An anti-protein-A modified microengine is finally used to demonstrate the selective capture, transport and convenient label-free optical detection of a Staphylococcus aureus target bacteria (containing proteinA in its cell wall) in the presence of a large excess of non-target (Saccharomyces cerevisiae) cells. The resulting nanomotor-based microchip immunoassay offers considerable potential for diverse applications in clinical diagnostics, environmental and security monitoring fields. Electronic supplementary information (ESI) available. See DOI: 10.1039/c2nr32400h

  19. Hybrid vehicle motor alignment

    DOEpatents

    Levin, Michael Benjamin

    2001-07-03

    A rotor of an electric motor for a motor vehicle is aligned to an axis of rotation for a crankshaft of an internal combustion engine having an internal combustion engine and an electric motor. A locator is provided on the crankshaft, a piloting tool is located radially by the first locator to the crankshaft. A stator of the electric motor is aligned to a second locator provided on the piloting tool. The stator is secured to the engine block. The rotor is aligned to the crankshaft and secured thereto.

  20. Putting engineering back into protein engineering: bioinformatic approaches to catalyst design.

    PubMed

    Gustafsson, Claes; Govindarajan, Sridhar; Minshull, Jeremy

    2003-08-01

    Complex multivariate engineering problems are commonplace and not unique to protein engineering. Mathematical and data-mining tools developed in other fields of engineering have now been applied to analyze sequence-activity relationships of peptides and proteins and to assist in the design of proteins and peptides with specified properties. Decreasing costs of DNA sequencing in conjunction with methods to quickly synthesize statistically representative sets of proteins allow modern heuristic statistics to be applied to protein engineering. This provides an alternative approach to expensive assays or unreliable high-throughput surrogate screens.

  1. 40 CFR 85.1708 - National security exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false National security exemption. 85.1708... Vehicle Engines § 85.1708 National security exemption. A manufacturer requesting a national security... agency of the Federal Government charged with responsibility for national defense. [39 FR 32611, Sept. 10...

  2. Development and Validation of Mechanical Engineering Trade Skills Assessment Instrument for Sustainable Job Security in Yobe State

    ERIC Educational Resources Information Center

    Adamu, Gishua Garba; Dawha, Josphine Musa; Kamar, Tiamiyu Salihu

    2015-01-01

    Mechanical Engineering Trade Skills Assessment Instrument (METSAI) is aimed at determining the extent to which students have acquired practical skills before graduation that will enable them get employment for sustainable job security in Yobe state. The study employed instrumentation research design. The populations of the study were 23 mechanical…

  3. 40 CFR 90.908 - National security exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false National security exemption. 90.908... Exemption of Nonroad Engines from Regulations § 90.908 National security exemption. (a)(1) Any nonroad... defense, will be considered exempt from this part for purposes of national security. No request for...

  4. 40 CFR 89.908 - National security exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false National security exemption. 89.908... Provisions § 89.908 National security exemption. (a)(1) Any nonroad engine, otherwise subject to this part... regulations for purposes of national security. No request for exemption is necessary. (2) Manufacturers may...

  5. Common Criteria Related Security Design Patterns for Intelligent Sensors—Knowledge Engineering-Based Implementation

    PubMed Central

    Bialas, Andrzej

    2011-01-01

    Intelligent sensors experience security problems very similar to those inherent to other kinds of IT products or systems. The assurance for these products or systems creation methodologies, like Common Criteria (ISO/IEC 15408) can be used to improve the robustness of the sensor systems in high risk environments. The paper presents the background and results of the previous research on patterns-based security specifications and introduces a new ontological approach. The elaborated ontology and knowledge base were validated on the IT security development process dealing with the sensor example. The contribution of the paper concerns the application of the knowledge engineering methodology to the previously developed Common Criteria compliant and pattern-based method for intelligent sensor security development. The issue presented in the paper has a broader significance in terms that it can solve information security problems in many application domains. PMID:22164064

  6. Common criteria related security design patterns for intelligent sensors--knowledge engineering-based implementation.

    PubMed

    Bialas, Andrzej

    2011-01-01

    Intelligent sensors experience security problems very similar to those inherent to other kinds of IT products or systems. The assurance for these products or systems creation methodologies, like Common Criteria (ISO/IEC 15408) can be used to improve the robustness of the sensor systems in high risk environments. The paper presents the background and results of the previous research on patterns-based security specifications and introduces a new ontological approach. The elaborated ontology and knowledge base were validated on the IT security development process dealing with the sensor example. The contribution of the paper concerns the application of the knowledge engineering methodology to the previously developed Common Criteria compliant and pattern-based method for intelligent sensor security development. The issue presented in the paper has a broader significance in terms that it can solve information security problems in many application domains.

  7. 40 CFR 91.1002 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

  8. 40 CFR 91.1002 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

  9. 40 CFR 91.1002 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

  10. 40 CFR 91.1002 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1002... means an exemption granted under § 91.1104(b) for the purpose of exporting new marine SI engines... national security. Manufacturer-owned marine engine means an uncertified marine SI engine owned and...

  11. Chemical Facility Security: Reauthorization, Policy Issues, and Options for Congress

    DTIC Science & Technology

    2009-07-13

    Process Safety, American Institute of Chemical Engineers , before the Senate Committee on Environment and Public Works, June 21, 2006, S.Hrg. 109-1044. See...example, Testimony by Dennis C. Hendershot, Staff Consultant, Center for Chemical Process Safety, American Institute of Chemical Engineers , before...CRS Report for Congress Prepared for Members and Committees of Congress Chemical Facility Security: Reauthorization, Policy Issues, and

  12. 47 CFR 0.332 - Actions taken under delegated authority.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... safety, homeland security, national security, emergency management and preparedness, and disaster management communications—the Public Safety and Homeland Security Bureau. (d) Complaints involving equal... frequencies shared with broadcast, common carrier, or government services—Office of Engineering and Technology...

  13. 46 CFR 113.27-1 - Engineers' assistance-needed alarm.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Engineers' assistance-needed alarm. 113.27-1 Section 113.27-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engineers' Assistance-Needed Alarm § 113.27-1 Engineers...

  14. 46 CFR 113.27-1 - Engineers' assistance-needed alarm.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Engineers' assistance-needed alarm. 113.27-1 Section 113.27-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engineers' Assistance-Needed Alarm § 113.27-1 Engineers...

  15. 46 CFR 113.27-1 - Engineers' assistance-needed alarm.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Engineers' assistance-needed alarm. 113.27-1 Section 113.27-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engineers' Assistance-Needed Alarm § 113.27-1 Engineers...

  16. 46 CFR 113.27-1 - Engineers' assistance-needed alarm.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Engineers' assistance-needed alarm. 113.27-1 Section 113.27-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engineers' Assistance-Needed Alarm § 113.27-1 Engineers...

  17. 46 CFR 113.27-1 - Engineers' assistance-needed alarm.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Engineers' assistance-needed alarm. 113.27-1 Section 113.27-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engineers' Assistance-Needed Alarm § 113.27-1 Engineers...

  18. Protein engineering and the use of molecular modeling and simulation: the case of heterodimeric Fc engineering.

    PubMed

    Spreter Von Kreudenstein, Thomas; Lario, Paula I; Dixit, Surjit B

    2014-01-01

    Computational and structure guided methods can make significant contributions to the development of solutions for difficult protein engineering problems, including the optimization of next generation of engineered antibodies. In this paper, we describe a contemporary industrial antibody engineering program, based on hypothesis-driven in silico protein optimization method. The foundational concepts and methods of computational protein engineering are discussed, and an example of a computational modeling and structure-guided protein engineering workflow is provided for the design of best-in-class heterodimeric Fc with high purity and favorable biophysical properties. We present the engineering rationale as well as structural and functional characterization data on these engineered designs. Copyright © 2013 Elsevier Inc. All rights reserved.

  19. Final Rule for Gasoline Spark-Ignition Marine Engines; Exemptions for New Nonroad Compression-Ignition Engines at or Above 37 Kilowatts and New Nonroad Spark-Ignition Engines at or Below 19 Kilowatts

    EPA Pesticide Factsheets

    These standards apply for outboard engines, personal watercraft engines, and jet boat engines. This rule also adds a national security exemption for Nonroad Compression-Ignition (CI) and Small SI sectors.

  20. Pharmaceutical and industrial protein engineering: where we are?

    PubMed

    Amara, Amro Abd-Al-Fattah

    2013-01-01

    The huge amount of information, the big number of scientists and their efforts, labs, man/hrs, fund, companies all and others factors build the success of the amazing new branch of genetic engineering the 'protein engineering' (PE). It concerns with the modification of protein structure/function(s) or building protein from scratch. The engineered proteins usually have new criteria(s). Engineering proteins can be mediated on the level of genes or proteins. PE fined its way in different important sectors including industrial, pharmaceutical and medicinal ones. Aspects about PE and its applications will be discussed with this review. The concept, tools, and the industrial applications of the protein, engineered proteins and PE will be under focus. In order to get up to date knowledge about the applications of PE in basic protein and molecular biology, several examples are discussed. PE can play a significant role in different industrial and pharmaceutical sectors if used wisely and selectively.

  1. Hardening Logic Encryption against Key Extraction Attacks with Circuit Camouflage

    DTIC Science & Technology

    2017-03-01

    camouflage; obfuscation; SAT; key extraction; reverse engineering; security; trusted electronics Introduction Integrated Circuit (IC) designs are...Encryption Algorithms”, Hardware Oriented Security and Trust , 2015. 3. Rajendran J., Pino, Y., Sinanoglu, O., Karri, R., “Security Analysis of Logic

  2. 46 CFR 113.35-9 - Mechanical engine order telegraph systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Mechanical engine order telegraph systems. 113.35-9 Section 113.35-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-9 Mechanical engine order...

  3. 46 CFR 113.35-9 - Mechanical engine order telegraph systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Mechanical engine order telegraph systems. 113.35-9 Section 113.35-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-9 Mechanical engine order...

  4. 46 CFR 113.35-9 - Mechanical engine order telegraph systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Mechanical engine order telegraph systems. 113.35-9 Section 113.35-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-9 Mechanical engine order...

  5. 46 CFR 113.35-9 - Mechanical engine order telegraph systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Mechanical engine order telegraph systems. 113.35-9 Section 113.35-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-9 Mechanical engine order...

  6. Utilizing Current Commercial-off-the-Shelf Facial Recognition and Public Live Video Streaming to Enhance National Security

    DTIC Science & Technology

    2014-09-01

    biometrics technologies. 14. SUBJECT TERMS Facial recognition, systems engineering, live video streaming, security cameras, national security ...national security by sharing biometric facial recognition data in real-time utilizing infrastructures currently in place. It should be noted that the...9/11),law enforcement (LE) and Intelligence community (IC)authorities responsible for protecting citizens from threats against national security

  7. Process Improvement Should Link to Security: SEPG 2007 Security Track Recap

    DTIC Science & Technology

    2007-09-01

    the Systems Security Engineering Capability Maturity Model (SSE- CMM / ISO 21827) and its use in system software developments ...software development life cycle ( SDLC )? 6. In what ways should process improvement support security in the SDLC ? 1.2 10BPANEL RESOURCES For each... project management, and support practices through the use of the capability maturity models including the CMMI and the Systems Security

  8. Introducing the CERT (Trademark) Resiliency Engineering Framework: Improving the Security and Sustainability Processes

    DTIC Science & Technology

    2007-05-01

    business processes and services. 4. Security operations management addresses the day-to-day activities that the organization performs to protect the...Management TM – Technology Management Security Operations Management SOM – Security Operations Management 5.7.2 Important Operations Competency...deals with the provision of access rights to informa- tion and technical assets SOM – Security Operations Management , which addresses the fundamental

  9. Chemical Facility Security: Reauthorization, Policy Issues, and Options for Congress

    DTIC Science & Technology

    2010-11-15

    American Institute of Chemical Engineers , before the Senate Committee on Environment and Public Works, June 21, 2006, S.Hrg. 109-1044. See also...American Institute of Chemical Engineers , before the Senate Committee on Environment and Public Works, June 21, 2006, S.Hrg. 109-1044. 57 The DHS...CRS Report for Congress Prepared for Members and Committees of Congress Chemical Facility Security: Reauthorization, Policy Issues, and

  10. Exploring Effects of Organizational Culture upon Implementation of Information Security Awareness and Training Programs within the Defense Industry Located in the Tennessee Valley Region

    ERIC Educational Resources Information Center

    Grant, Robert Luther

    2017-01-01

    Data breaches due to social engineering attacks and employee negligence are on the rise. The only known defense against social engineering attacks and employee negligence is information security awareness and training. However, implementation of awareness and training programs within organizations are lagging in priority. This research used the…

  11. A Systematic Comprehensive Computational Model for Stake Estimation in Mission Assurance: Applying Cyber Security Econometrics System (CSES) to Mission Assurance Analysis Protocol (MAAP)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Abercrombie, Robert K; Sheldon, Frederick T; Grimaila, Michael R

    2010-01-01

    In earlier works, we presented a computational infrastructure that allows an analyst to estimate the security of a system in terms of the loss that each stakeholder stands to sustain as a result of security breakdowns. In this paper, we discuss how this infrastructure can be used in the subject domain of mission assurance as defined as the full life-cycle engineering process to identify and mitigate design, production, test, and field support deficiencies of mission success. We address the opportunity to apply the Cyberspace Security Econometrics System (CSES) to Carnegie Mellon University and Software Engineering Institute s Mission Assurance Analysismore » Protocol (MAAP) in this context.« less

  12. 77 FR 56662 - Homeland Security Science and Technology Advisory Committee (HSSTAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-13

    ... as new developments in systems engineering, cyber-security, knowledge management and how best to... Management; Notice of Federal Advisory Committee Meeting. SUMMARY: The Homeland Security Science and... the Under Secretary on policies, management processes, and organizational constructs as needed. Agenda...

  13. 78 FR 66949 - Homeland Security Science and Technology Advisory Committee (HSSTAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-07

    ... Technology, such as new developments in systems engineering, cyber-security, knowledge management and how... Management; Notice of Federal Advisory Committee Meeting. SUMMARY: The Homeland Security Science and... also advises the Under Secretary on policies, management processes, and organizational constructs as...

  14. Protein Engineering Approaches in the Post-Genomic Era.

    PubMed

    Singh, Raushan K; Lee, Jung-Kul; Selvaraj, Chandrabose; Singh, Ranjitha; Li, Jinglin; Kim, Sang-Yong; Kalia, Vipin C

    2018-01-01

    Proteins are one of the most multifaceted macromolecules in living systems. Proteins have evolved to function under physiological conditions and, therefore, are not usually tolerant of harsh experimental and environmental conditions. The growing use of proteins in industrial processes as a greener alternative to chemical catalysts often demands constant innovation to improve their performance. Protein engineering aims to design new proteins or modify the sequence of a protein to create proteins with new or desirable functions. With the emergence of structural and functional genomics, protein engineering has been invigorated in the post-genomic era. The three-dimensional structures of proteins with known functions facilitate protein engineering approaches to design variants with desired properties. There are three major approaches of protein engineering research, namely, directed evolution, rational design, and de novo design. Rational design is an effective method of protein engineering when the threedimensional structure and mechanism of the protein is well known. In contrast, directed evolution does not require extensive information and a three-dimensional structure of the protein of interest. Instead, it involves random mutagenesis and selection to screen enzymes with desired properties. De novo design uses computational protein design algorithms to tailor synthetic proteins by using the three-dimensional structures of natural proteins and their folding rules. The present review highlights and summarizes recent protein engineering approaches, and their challenges and limitations in the post-genomic era. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  15. Applications of yeast surface display for protein engineering

    PubMed Central

    Cherf, Gerald M.; Cochran, Jennifer R.

    2015-01-01

    The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine. PMID:26060074

  16. 40 CFR 89.902 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... EMISSIONS FROM NEW AND IN-USE NONROAD COMPRESSION-IGNITION ENGINES Exemption Provisions § 89.902 Definitions... an exemption granted under § 89.1004(b) for the purpose of exporting new nonroad engines. National... security. Manufacturer-owned nonroad engine means an uncertified nonroad engine owned and controlled by a...

  17. 40 CFR 89.902 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... EMISSIONS FROM NEW AND IN-USE NONROAD COMPRESSION-IGNITION ENGINES Exemption Provisions § 89.902 Definitions... an exemption granted under § 89.1004(b) for the purpose of exporting new nonroad engines. National... security. Manufacturer-owned nonroad engine means an uncertified nonroad engine owned and controlled by a...

  18. 40 CFR 89.902 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... EMISSIONS FROM NEW AND IN-USE NONROAD COMPRESSION-IGNITION ENGINES Exemption Provisions § 89.902 Definitions... an exemption granted under § 89.1004(b) for the purpose of exporting new nonroad engines. National... security. Manufacturer-owned nonroad engine means an uncertified nonroad engine owned and controlled by a...

  19. 40 CFR 89.902 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... EMISSIONS FROM NEW AND IN-USE NONROAD COMPRESSION-IGNITION ENGINES Exemption Provisions § 89.902 Definitions... an exemption granted under § 89.1004(b) for the purpose of exporting new nonroad engines. National... security. Manufacturer-owned nonroad engine means an uncertified nonroad engine owned and controlled by a...

  20. 46 CFR 15.825 - Engineers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Engineers. 15.825 Section 15.825 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.825 Engineers. (a) An individual in charge of an engineering watch on a mechanically propelled...

  1. 46 CFR 129.560 - Engine-order telegraphs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

  2. 46 CFR 11.505 - Engineer officer structure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Engineer officer structure. 11.505 Section 11.505 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN REQUIREMENTS FOR OFFICER ENDORSEMENTS Professional Requirements for Engineer Officer § 11.505 Engineer officer structure...

  3. 46 CFR 11.505 - Engineer officer structure.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Engineer officer structure. 11.505 Section 11.505 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN REQUIREMENTS FOR OFFICER ENDORSEMENTS Professional Requirements for Engineer Officer § 11.505 Engineer officer structure...

  4. 46 CFR 11.505 - Engineer officer structure.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Engineer officer structure. 11.505 Section 11.505 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN REQUIREMENTS FOR OFFICER ENDORSEMENTS Professional Requirements for Engineer Officer § 11.505 Engineer officer structure...

  5. 46 CFR 11.505 - Engineer officer structure.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Engineer officer structure. 11.505 Section 11.505 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN REQUIREMENTS FOR OFFICER ENDORSEMENTS Professional Requirements for Engineer Officer § 11.505 Engineer officer structure...

  6. 46 CFR 15.825 - Engineers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Engineers. 15.825 Section 15.825 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.825 Engineers. (a) An individual in charge of an engineering watch on a mechanically propelled...

  7. 46 CFR 15.825 - Engineers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Engineers. 15.825 Section 15.825 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.825 Engineers. (a) An individual in charge of an engineering watch on a mechanically propelled...

  8. 46 CFR 15.825 - Engineers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Engineers. 15.825 Section 15.825 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.825 Engineers. (a) An individual in charge of an engineering watch on a mechanically propelled...

  9. 46 CFR 15.825 - Engineers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Engineers. 15.825 Section 15.825 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.825 Engineers. (a) An individual in charge of an engineering watch on a mechanically propelled...

  10. 46 CFR 129.560 - Engine-order telegraphs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

  11. 46 CFR 129.560 - Engine-order telegraphs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

  12. 46 CFR 129.560 - Engine-order telegraphs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Engine-order telegraphs. 129.560 Section 129.560 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS ELECTRICAL INSTALLATIONS Miscellaneous Electrical Systems § 129.560 Engine-order telegraphs. No OSV need carry an engine...

  13. Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry.

    PubMed

    Gao, Xiaoye; Fang, Jie; Xue, Bin; Fu, Linglan; Li, Hongbin

    2016-09-12

    Constructing hydrogels from engineered proteins has attracted significant attention within the material sciences, owing to their myriad potential applications in biomedical engineering. Developing efficient methods to cross-link tailored protein building blocks into hydrogels with desirable mechanical, physical, and functional properties is of paramount importance. By making use of the recently developed SpyCatcher-SpyTag chemistry, we successfully engineered protein hydrogels on the basis of engineered tandem modular elastomeric proteins. Our resultant protein hydrogels are soft but stable, and show excellent biocompatibility. As the first step, we tested the use of these hydrogels as a drug carrier, as well as in encapsulating human lung fibroblast cells. Our results demonstrate the robustness of the SpyCatcher-SpyTag chemistry, even when the SpyTag (or SpyCatcher) is flanked by folded globular domains. These results demonstrate that SpyCatcher-SpyTag chemistry can be used to engineer protein hydrogels from tandem modular elastomeric proteins that can find applications in tissue engineering, in fundamental mechano-biological studies, and as a controlled drug release vehicle.

  14. A Summary of the Naval Postgraduate School Research Program.

    DTIC Science & Technology

    1984-06-01

    Administrative Sciences, Operations Research, National Security Affairs, Physics, Electrical Engineering , Meterology, Aeronautics, Oceanography and Mechanical ...Oceans and Major Seas -------------------------------- 290 DEPARTMENT OF MECHANICAL ENGINEERING 291 Mechanical Engineering Department Summary 293...in Buried Pipes Using Sulphur Hexaflouride as a Tracer Gas," American Society of Mechanical Engineers , The Journal of Engineering for Power

  15. Protein engineering for metabolic engineering: current and next-generation tools

    PubMed Central

    Marcheschi, Ryan J.; Gronenberg, Luisa S.; Liao, James C.

    2014-01-01

    Protein engineering in the context of metabolic engineering is increasingly important to the field of industrial biotechnology. As the demand for biologically-produced food, fuels, chemicals, food additives, and pharmaceuticals continues to grow, the ability to design and modify proteins to accomplish new functions will be required to meet the high productivity demands for the metabolism of engineered organisms. This article reviews advances of selecting, modeling, and engineering proteins to improve or alter their activity. Some of the methods have only recently been developed for general use and are just beginning to find greater application in the metabolic engineering community. We also discuss methods of generating random and targeted diversity in proteins to generate mutant libraries for analysis. Recent uses of these techniques to alter cofactor use, produce non-natural amino acids, alcohols, and carboxylic acids, and alter organism phenotypes are presented and discussed as examples of the successful engineering of proteins for metabolic engineering purposes. PMID:23589443

  16. Protein engineering for metabolic engineering: current and next-generation tools.

    PubMed

    Marcheschi, Ryan J; Gronenberg, Luisa S; Liao, James C

    2013-05-01

    Protein engineering in the context of metabolic engineering is increasingly important to the field of industrial biotechnology. As the demand for biologically produced food, fuels, chemicals, food additives, and pharmaceuticals continues to grow, the ability to design and modify proteins to accomplish new functions will be required to meet the high productivity demands for the metabolism of engineered organisms. We review advances in selecting, modeling, and engineering proteins to improve or alter their activity. Some of the methods have only recently been developed for general use and are just beginning to find greater application in the metabolic engineering community. We also discuss methods of generating random and targeted diversity in proteins to generate mutant libraries for analysis. Recent uses of these techniques to alter cofactor use; produce non-natural amino acids, alcohols, and carboxylic acids; and alter organism phenotypes are presented and discussed as examples of the successful engineering of proteins for metabolic engineering purposes. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Engineering Institute

    Science.gov Websites

    Search Site submit National Security Education Center Los Alamos National LaboratoryEngineering Institute Addressing national needs by fostering specialized recruiting and strategic partnerships Los Alamos National LaboratoryEngineering Institute Menu NSEC Educational Programs Los Alamos Dynamics Summer

  18. Security Primitives for Reconfigurable Hardware-Based Systems

    DTIC Science & Technology

    2010-05-01

    work, we propose security primitives using ideas centered around the notion of “moats and drawbridges .” The primitives encompass four design properties...Santa Bar- bara, CA 93106; email: sherwood@cs.ucsb.edu; R. Kastner, Department of Computer Science and Engineering , University of California, San...fingerprint reader), the other to control the ethernet IP core—and an AES encryption engine used by both of the processor cores. These cores are all implemented

  19. Suggestions for Strategic Planning for the Office of Nonproliferation Research and Engineering

    DTIC Science & Technology

    2002-01-01

    The Office of Nonproliferation Research and Engineering, part of the recently formed National Nuclear Security Administration within the Department...of Energy, conducts wide-ranging research for diverse end users at the federal and local level. As such, the Office faces a number of challenges in...planning methods. This report grew out of support provided to an advisory committee for the Office of Nonproliferation and National Security, an

  20. 33 CFR 165.768 - Security Zone; MacDill Air Force Base, Tampa Bay, FL.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Security Zone; MacDill Air Force....768 Security Zone; MacDill Air Force Base, Tampa Bay, FL. (a) Location. The following area is a security zone which exists concurrent with an Army Corps of Engineers restricted area in § 334.635 of this...

  1. 33 CFR 165.768 - Security Zone; MacDill Air Force Base, Tampa Bay, FL.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Security Zone; MacDill Air Force....768 Security Zone; MacDill Air Force Base, Tampa Bay, FL. (a) Location. The following area is a security zone which exists concurrent with an Army Corps of Engineers restricted area in § 334.635 of this...

  2. 33 CFR 165.768 - Security Zone; MacDill Air Force Base, Tampa Bay, FL.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Security Zone; MacDill Air Force....768 Security Zone; MacDill Air Force Base, Tampa Bay, FL. (a) Location. The following area is a security zone which exists concurrent with an Army Corps of Engineers restricted area in § 334.635 of this...

  3. 33 CFR 165.768 - Security Zone; MacDill Air Force Base, Tampa Bay, FL.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Security Zone; MacDill Air Force....768 Security Zone; MacDill Air Force Base, Tampa Bay, FL. (a) Location. The following area is a security zone which exists concurrent with an Army Corps of Engineers restricted area in § 334.635 of this...

  4. 46 CFR 11.540 - Endorsements for engineers of mobile offshore drilling units.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Endorsements for engineers of mobile offshore drilling units. 11.540 Section 11.540 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE... § 11.540 Endorsements for engineers of mobile offshore drilling units. Endorsements as chief engineer...

  5. 46 CFR 11.540 - Endorsements for engineers of mobile offshore drilling units.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Endorsements for engineers of mobile offshore drilling units. 11.540 Section 11.540 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE... § 11.540 Endorsements for engineers of mobile offshore drilling units. Endorsements as chief engineer...

  6. 46 CFR 11.540 - Endorsements for engineers of mobile offshore drilling units.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Endorsements for engineers of mobile offshore drilling units. 11.540 Section 11.540 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE... § 11.540 Endorsements for engineers of mobile offshore drilling units. Endorsements as chief engineer...

  7. 46 CFR 11.540 - Endorsements for engineers of mobile offshore drilling units.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Endorsements for engineers of mobile offshore drilling units. 11.540 Section 11.540 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE... § 11.540 Endorsements for engineers of mobile offshore drilling units. Endorsements as chief engineer...

  8. 40 CFR 85.1702 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... POLLUTION FROM MOBILE SOURCES Exclusion and Exemption of Motor Vehicles and Motor Vehicle Engines § 85.1702... for the purpose of exporting new motor vehicles or new motor vehicle engines. (2) National security...-certification vehicle engine means an uncertified heavy-duty engine owned by a manufacturer and used in a manner...

  9. 40 CFR 85.1702 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... POLLUTION FROM MOBILE SOURCES Exclusion and Exemption of Motor Vehicles and Motor Vehicle Engines § 85.1702... for the purpose of exporting new motor vehicles or new motor vehicle engines. (2) National security...-certification vehicle engine means an uncertified heavy-duty engine owned by a manufacturer and used in a manner...

  10. 40 CFR 85.1702 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... POLLUTION FROM MOBILE SOURCES Exclusion and Exemption of Motor Vehicles and Motor Vehicle Engines § 85.1702... for the purpose of exporting new motor vehicles or new motor vehicle engines. (2) National security...-certification vehicle engine means an uncertified heavy-duty engine owned by a manufacturer and used in a manner...

  11. 40 CFR 85.1702 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... POLLUTION FROM MOBILE SOURCES Exclusion and Exemption of Motor Vehicles and Motor Vehicle Engines § 85.1702... for the purpose of exporting new motor vehicles or new motor vehicle engines. (2) National security...-certification vehicle engine means an uncertified heavy-duty engine owned by a manufacturer and used in a manner...

  12. 77 FR 21587 - Bayer Cropscience, LP, Including On-Site Leased Workers From Jacobs PSG, Middough Associates, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-10

    ...., Digital Management Group, Mercury Air Group, Inc., Greenwood, and Professional Maintenance of Charleston... Solutions, Becht Engineering, Engineering Support Systems, Manufacturing Management Services, US Securities, WB Wells, Belcan, American Engineers, CH2M Hill Engineers, Inc., Digital Management Group, Mercury...

  13. 46 CFR 62.35-35 - Starting systems for internal-combustion engines.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Starting systems for internal-combustion engines. 62.35-35 Section 62.35-35 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE... Starting systems for internal-combustion engines. The starting systems for propulsion engines and for prime...

  14. 46 CFR 62.35-35 - Starting systems for internal-combustion engines.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Starting systems for internal-combustion engines. 62.35-35 Section 62.35-35 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE... Starting systems for internal-combustion engines. The starting systems for propulsion engines and for prime...

  15. 46 CFR 62.35-35 - Starting systems for internal-combustion engines.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Starting systems for internal-combustion engines. 62.35-35 Section 62.35-35 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE... Starting systems for internal-combustion engines. The starting systems for propulsion engines and for prime...

  16. 46 CFR 62.35-35 - Starting systems for internal-combustion engines.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Starting systems for internal-combustion engines. 62.35-35 Section 62.35-35 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE... Starting systems for internal-combustion engines. The starting systems for propulsion engines and for prime...

  17. 46 CFR 62.35-35 - Starting systems for internal-combustion engines.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Starting systems for internal-combustion engines. 62.35-35 Section 62.35-35 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE... Starting systems for internal-combustion engines. The starting systems for propulsion engines and for prime...

  18. 40 CFR 1042.635 - National security exemption.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Special... CONTROL INFORMATION”. (2) Your corporate name and trademark. (3) Engine displacement, family... prohibitions in § 1068.101(a)(1) do not apply to engines exempted under this section. (a) An engine is exempt...

  19. 40 CFR 1042.635 - National security exemption.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Special... CONTROL INFORMATION”. (2) Your corporate name and trademark. (3) Engine displacement, family... prohibitions in § 1068.101(a)(1) do not apply to engines exempted under this section. (a) An engine is exempt...

  20. 40 CFR 1042.635 - National security exemption.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Special... CONTROL INFORMATION”. (2) Your corporate name and trademark. (3) Engine displacement, family... prohibitions in § 1068.101(a)(1) do not apply to engines exempted under this section. (a) An engine is exempt...

  1. 40 CFR 1042.635 - National security exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Special... CONTROL INFORMATION”. (2) Your corporate name and trademark. (3) Engine displacement, family... prohibitions in § 1068.101(a)(1) do not apply to engines exempted under this section. (a) An engine is exempt...

  2. 40 CFR 1042.635 - National security exemption.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Special... CONTROL INFORMATION”. (2) Your corporate name and trademark. (3) Engine displacement, family... prohibitions in § 1068.101(a)(1) do not apply to engines exempted under this section. (a) An engine is exempt...

  3. 46 CFR 31.35-5 - Communications; alarm systems, telephone and voice tube systems, engine telegraph systems, etc...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... OF HOMELAND SECURITY TANK VESSELS INSPECTION AND CERTIFICATION Electrical Engineering § 31.35-5... vessels are subject to the regulations contained in subchapter J (Electrical Engineering) of this chapter...

  4. 46 CFR 31.35-5 - Communications; alarm systems, telephone and voice tube systems, engine telegraph systems, etc...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... OF HOMELAND SECURITY TANK VESSELS INSPECTION AND CERTIFICATION Electrical Engineering § 31.35-5... vessels are subject to the regulations contained in subchapter J (Electrical Engineering) of this chapter...

  5. 46 CFR 31.35-5 - Communications; alarm systems, telephone and voice tube systems, engine telegraph systems, etc...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... OF HOMELAND SECURITY TANK VESSELS INSPECTION AND CERTIFICATION Electrical Engineering § 31.35-5... vessels are subject to the regulations contained in subchapter J (Electrical Engineering) of this chapter...

  6. Protein Engineering: Case Studies of Commercialized Engineered Products

    ERIC Educational Resources Information Center

    Walsh, Gary

    2007-01-01

    Programs in biochemistry invariably encompass the principles of protein engineering. Students often display increased understanding and enthusiasm when theoretical concepts are underpinned by practical example. Herein are presented five case studies, each focusing upon a commercial protein product engineered to enhance its application-relevant…

  7. Protein engineering approaches to chemical biotechnology.

    PubMed

    Chen, Zhen; Zeng, An-Ping

    2016-12-01

    Protein engineering for the improvement of properties of biocatalysts and for the generation of novel metabolic pathways plays more and more important roles in chemical biotechnology aiming at the production of chemicals from biomass. Although widely used in single-enzyme catalysis process, protein engineering is only being increasingly explored in recent years to achieve more complex in vitro and in vivo biocatalytic processes. This review focuses on major contributions of protein engineering to chemical biotechnology in the field of multi-enzymatic cascade catalysis and metabolic engineering. Especially, we discuss and highlight recent strategies for combining pathway design and protein engineering for the production of novel products. Copyright © 2016. Published by Elsevier Ltd.

  8. 77 FR 14955 - DoD Information Assurance Scholarship Program (IASP)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-14

    ... IA and information technology (IT) management, technical, digital and multimedia forensics, cyber..., digital and multimedia forensics, electrical engineering, electronics engineering, information security...

  9. CrossTalk: The Journal of Defense Software Engineering. Volume 20, Number 3, March 2007

    DTIC Science & Technology

    2007-03-01

    Capability Maturity Model ® Integration (CMMI®). CMU Software Engineering Institute <www.sei.cmu.edu/cmmi>. 5. ISO /IEC 27001 :2005. Information Security...international standards bodies – International Organization for Standardi- zation ( ISO ) and International Electro- technical Commission (IEC) – are working on a...number of projects that affect soft- ware security: • The ISO Technical Management Board (TMB) performs strategic planning and coordination for ISO

  10. An Artificial Neural Network-Based Decision-Support System for Integrated Network Security

    DTIC Science & Technology

    2014-09-01

    group that they need to know in order to make team-based decisions in real-time environments, (c) Employ secure cloud computing services to host mobile...THESIS Presented to the Faculty Department of Electrical and Computer Engineering Graduate School of Engineering and Management Air Force...out-of-the-loop syndrome and create complexity creep. As a result, full automation efforts can lead to inappropriate decision-making despite a

  11. The Need for Cyber-Informed Engineering Expertise for Nuclear Research Reactors

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Anderson, Robert Stephen

    Engineering disciplines may not currently understand or fully embrace cyber security aspects as they apply towards analysis, design, operation, and maintenance of nuclear research reactors. Research reactors include a wide range of diverse co-located facilities and designs necessary to meet specific operational research objectives. Because of the nature of research reactors (reduced thermal energy and fission product inventory), hazards and risks may not have received the same scrutiny as normally associated with power reactors. Similarly, security may not have been emphasized either. However, the lack of sound cybersecurity defenses may lead to both safety and security impacts. Risk management methodologiesmore » may not contain the foundational assumptions required to address the intelligent adversary’s capabilities in malevolent cyber attacks. Although most research reactors are old and may not have the same digital footprint as newer facilities, any digital instrument and control function must be considered as a potential attack platform that can lead to sabotage or theft of nuclear material, especially for some research reactors that store highly enriched uranium. This paper will provide a discussion about the need for cyber-informed engineering practices that include the entire engineering lifecycle. Cyber-informed engineering as referenced in this paper is the inclusion of cybersecurity aspects into the engineering process. A discussion will consider several attributes of this process evaluating the long-term goal of developing additional cyber safety basis analysis and trust principles. With a culture of free information sharing exchanges, and potentially a lack of security expertise, new risk analysis and design methodologies need to be developed to address this rapidly evolving (cyber) threatscape.« less

  12. 46 CFR 111.01-15 - Temperature ratings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS... assumed for all rotating electrical machinery in boiler rooms, engine rooms, auxiliary machinery rooms...-rotating electrical equipment in boiler rooms, in engine rooms, in auxiliary machinery rooms, and on...

  13. 46 CFR 111.01-15 - Temperature ratings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS... is assumed for all rotating electrical machinery in boiler rooms, engine rooms, auxiliary machinery...-rotating electrical equipment in boiler rooms, in engine rooms, in auxiliary machinery rooms, and on...

  14. 46 CFR 111.01-15 - Temperature ratings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS... is assumed for all rotating electrical machinery in boiler rooms, engine rooms, auxiliary machinery...-rotating electrical equipment in boiler rooms, in engine rooms, in auxiliary machinery rooms, and on...

  15. 46 CFR 111.01-15 - Temperature ratings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS... is assumed for all rotating electrical machinery in boiler rooms, engine rooms, auxiliary machinery...-rotating electrical equipment in boiler rooms, in engine rooms, in auxiliary machinery rooms, and on...

  16. 46 CFR 111.01-15 - Temperature ratings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS... assumed for all rotating electrical machinery in boiler rooms, engine rooms, auxiliary machinery rooms...-rotating electrical equipment in boiler rooms, in engine rooms, in auxiliary machinery rooms, and on...

  17. Micromotor-based lab-on-chip immunoassays.

    PubMed

    García, Miguel; Orozco, Jahir; Guix, Maria; Gao, Wei; Sattayasamitsathit, Sirilak; Escarpa, Alberto; Merkoçi, Arben; Wang, Joseph

    2013-02-21

    Here we describe the first example of using self-propelled antibody-functionalized synthetic catalytic microengines for capturing and transporting target proteins between the different reservoirs of a lab-on-a-chip (LOC) device. A new catalytic polymer/Ni/Pt microtube engine, containing carboxy moieties on its mixed poly(3,4-ethylenedioxythiophene) (PEDOT)/COOH-PEDOT polymeric outermost layer, is further functionalized with the antibody receptor to selectively recognize and capture the target protein. The new motor-based microchip immunoassay operations are carried out without any bulk fluid flow, replacing the common washing steps in antibody-based protein bioassays with the active transport of the captured protein throughout the different reservoirs, where each step of the immunoassay takes place. A first microchip format involving an 'on-the-fly' double-antibody sandwich assay (DASA) is used for demonstrating the selective capture of the target protein, in the presence of excess of non-target proteins. A secondary antibody tagged with a polymeric-sphere tracer allows the direct visualization of the binding events. In a second approach the immuno-nanomotor captures and transports the microsphere-tagged antigen through a microchannel network. An anti-protein-A modified microengine is finally used to demonstrate the selective capture, transport and convenient label-free optical detection of a Staphylococcus aureus target bacteria (containing proteinA in its cell wall) in the presence of a large excess of non-target (Saccharomyces cerevisiae) cells. The resulting nanomotor-based microchip immunoassay offers considerable potential for diverse applications in clinical diagnostics, environmental and security monitoring fields.

  18. 48 CFR 1804.470-3 - IT security requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the provisioning of services or products (e.g., research and development, engineering, manufacturing... Policies are available at the NASA IT Security Policy Web site at: http://www.nasa.gov/offices/ocio...

  19. 48 CFR 52.204-2 - Security Requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Agreement (DD Form 441), including the National Industrial Security Program Operating Manual (DOD 5220.22-M... architect-engineer contract, add the following paragraph (e) to the basic clause: (e) The Contractor shall...

  20. 46 CFR 11.540 - Endorsements as engineers of mobile offshore drilling units (MODUs).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Endorsements as engineers of mobile offshore drilling units (MODUs). 11.540 Section 11.540 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT... Engineer Officer Endorsements § 11.540 Endorsements as engineers of mobile offshore drilling units (MODUs...

  1. HCCI Combustion Engines Final Report CRADA No. TC02032.0

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Aceves, S.; Lyford-Pike, E.

    This was a collaborative effort between Lawrence Livermore National Security, LLC (formerly The Regents of the University of California)/Lawrence Livermore National Laboratory (LLNL) and Cummins Engine Company (Cwnmins), to advance the state of the art on HomogeneousCharge Compression-Ignition (HCCI) engines, resulting in a clean, high-efficiency alternative to diesel engines.

  2. 46 CFR 58.10-10 - Diesel engine installations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Diesel engine installations. 58.10-10 Section 58.10-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MAIN AND AUXILIARY... installations. (a) The requirements of § 58.10-5 (a), (c), and (d) shall apply to diesel engine installations...

  3. Results in Developing an Engineering Degree Program in Safeguards and Security of Nuclear Materials at Moscow Engineering Physics Institute

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kryuchkov, Eduard F.; Geraskin, Nikolay I.; Killinger, Mark H.

    The world’s first master’s degree program in nuclear safeguards and security, established at Moscow Engineering Physics Institute (MEPhI), has now graduated nine classes of students. Most of the graduates have gone on to work at government agencies, research organizations, or obtain their PhD. In order to meet the demand for safeguards and security specialists at nuclear facilities, MEPhI established a 5½ year engineering degree program that provides more hands-on training desired by facilities. In February 2004, the first students began their studies in the new discipline Nuclear Material Safeguards and Nonproliferation. This class, as well as other subsequent classes, includedmore » students who started the program in their third year of studies, as the first 2½ years consists of general engineering curriculum. Fourteen students made up the first graduating class, receiving their engineering degrees in February 2007. The topics addressed in this paper include specific features of the program caused by peculiarities of Russian education legislation and government quality control of academic education. This paper summarizes the main joint actions undertaken by MEPhI and the US National Laboratories in conjunction with the U.S. Department of Energy, to develop the engineering degree program. Also discussed are the program’s specific training requirements, student internships, and job placement. The paper concludes with recommendations from a recent international seminar on nonproliferation education and training.« less

  4. KIVA-hpFE. Predictive turbulent reactive and multiphase flow in engines - An Overview

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Carrington, David Bradley

    2016-05-23

    Research and development of KIVA-hpFE for turbulent reactive and multiphase flow particularly as related to engine modeling program has relevance to National energy security and climate change. Climate change is a source problem, and energy national security is consumption of petroleum products problem. Accurately predicting engine processes leads to, lower greenhouse gas (GHG) emission, where engines in the transportation sector currently account for 26% of the U.S. GHG emissions. Less dependence on petroleum products leads to greater energy security. By Environmental Protection Agency standards, some vehicles are now reaching 42 to the 50 mpg mark. These are conventional gasoline engines.more » Continued investment and research into new technical innovations, the potential exists to save more than 4 million barrels of oil per day or approximately $200 to $400 million per day. This would be a significant decrease in emission and use of petroleum and a very large economic stimulus too! It is estimated with further advancements in combustion, the current emissions can be reduced up to 40%. Enabling better understanding of fuel injection and fuel-air mixing, thermodynamic combustion losses, and combustion/emission formation processes enhances our ability to help solve both problems. To provide adequate capability for accurately simulating these processes, minimize time and labor for development of engine technology, are the goals of our KIVA development program.« less

  5. Social engineering: mitigating a stealthy risk.

    PubMed

    Maas, Jos

    2014-01-01

    Can a Healthcare Facility (HCF) be victimized by Social Engineering (SE)? Yes, says the author If so, what can you do about it? This article explains what Social Engineering is; how it is used; and how to use proactive security to prevent such an attack.

  6. 14 CFR 49.51 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Air Carrier Aircraft Engines, Propellers... aircraft engine, propeller, or appliance maintained by or on behalf of an air carrier certificated under 49 U.S.C. 44705 for installation or use in aircraft, aircraft engines, or propellers, or any spare...

  7. 14 CFR 49.51 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Air Carrier Aircraft Engines, Propellers... aircraft engine, propeller, or appliance maintained by or on behalf of an air carrier certificated under 49 U.S.C. 44705 for installation or use in aircraft, aircraft engines, or propellers, or any spare...

  8. 14 CFR 49.51 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Air Carrier Aircraft Engines, Propellers... aircraft engine, propeller, or appliance maintained by or on behalf of an air carrier certificated under 49 U.S.C. 44705 for installation or use in aircraft, aircraft engines, or propellers, or any spare...

  9. 14 CFR 49.51 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Air Carrier Aircraft Engines, Propellers... aircraft engine, propeller, or appliance maintained by or on behalf of an air carrier certificated under 49 U.S.C. 44705 for installation or use in aircraft, aircraft engines, or propellers, or any spare...

  10. 14 CFR 49.51 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Air Carrier Aircraft Engines, Propellers... aircraft engine, propeller, or appliance maintained by or on behalf of an air carrier certificated under 49 U.S.C. 44705 for installation or use in aircraft, aircraft engines, or propellers, or any spare...

  11. Biotechnology Conference: Protein Engineering Held in Oxford, United Kingdom on 5-8 April 1987.

    DTIC Science & Technology

    1987-07-27

    engineered by protein engineering was reported by J. new variants which are now being checked. Brange (Novo Research Institute, Bags- Studies of a cassette...to Brange . Therefore, multidomain protein consisting of five Brange and his group applied protein en- putative domains: the fribonectin finger

  12. Introducing the CERT (Trademark) Resiliency Engineering Framework: Improving the Security and Sustainability Processes

    DTIC Science & Technology

    2007-05-01

    Organizational Structure 40 6.1.3 Funding Model 40 6.1.4 Role of Information Technology 40 6.2 Considering Process Improvement 41 6.2.1 Dimensions of...to the process definition for resiliency engineering. 6.1.3 Funding Model Just as organizational structures tend to align across security and...responsibility. Adopting an enter- prise view of operational resiliency and a process improvement approach requires that the funding model evolve to one

  13. Architecture and Assessment: Privacy Preserving Biometrically Secured Electronic Documents

    DTIC Science & Technology

    2015-01-01

    very large public and private fingerprint databases comprehensive risk analysis and system security contribution to developing international ...Safety and Security Program which is led by Defence Research and Development Canada’s Centre for Security Science, in partnership with Public Safety...201 © Sa Majesté la Reine (en droit du Canada), telle que représentée par le ministre de la Défense nationale, 201 Science and Engineering

  14. Mobile Security: A Systems Engineering Framework for Implementing Bring Your Own Device (BYOD) Security through the Combination of Policy Management and Technology

    ERIC Educational Resources Information Center

    Zahadat, Nima

    2016-01-01

    With the rapid increase of smartphones and tablets, security concerns have also been on the rise. Traditionally, Information Technology (IT) departments set up devices, apply security, and monitor them. Such approaches do not apply to today's mobile devices due to a phenomenon called Bring Your Own Device or BYOD. Employees find it desirable to…

  15. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Carrington, David Bradley; Waters, Jiajia

    Research and development of KIVA-hpFE for turbulent reactive and multiphase flow particularly as related to engine modeling program has relevance to National energy security and climate change. Climate change is a source problem, and energy national security is consumption of petroleum products problem. Accurately predicting engine processes leads to, lower greenhouse gas (GHG) emission, where engines in the transportation sector currently account for 26% of the U.S. GHG emissions. Less dependence on petroleum products leads to greater energy security. By Environmental Protection Agency standards, some vehicles are now reaching 42 to the 50 mpg mark. These are conventional gasoline engines.more » Continued investment and research into new technical innovations, the potential exists to save more than 4 million barrels of oil per day or approximately $200 to $400 million per day. This would be a significant decrease in emission and use of petroleum and a very large economic stimulus too! It is estimated with further advancements in combustion, the current emissions can be reduced up to 40%. Enabling better understanding of fuel injection and fuel-air mixing, thermodynamic combustion losses, and combustion/emission formation processes enhances our ability to help solve both problems. To provide adequate capability for accurately simulating these processes, minimize time and labor for development of engine technology, are the goals of our KIVA development program.« less

  16. 46 CFR 15.915 - Engineer Officer Endorsements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Engineer Officer Endorsements. 15.915 Section 15.915 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Equivalents § 15.915 Engineer Officer Endorsements. The following licenses and MMC officer...

  17. 46 CFR 59.01-2 - Incorporation by reference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS... Engineering Standards (CG-521), 2100 2nd St., SW., Stop 7126, Washington, DC 20593-7126, and is available from the sources listed below. (b) American Society of Mechanical Engineers (ASME) International, Three...

  18. 46 CFR 59.01-2 - Incorporation by reference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS... Engineering Standards (CG-ENG), 2100 2nd St., SW., Stop 7126, Washington, DC 20593-7126, and is available from the sources listed below. (b) American Society of Mechanical Engineers (ASME) International, Three...

  19. 46 CFR 59.01-2 - Incorporation by reference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS...-ENG), Attn: Office of Design and Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther... below. (b) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York...

  20. 46 CFR 59.01-2 - Incorporation by reference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS...-ENG), Attn: Office of Design and Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther... below. (b) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York...

  1. 46 CFR 59.01-2 - Incorporation by reference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING REPAIRS TO BOILERS... Engineering Standards (CG-521), 2100 2nd St., SW., Stop 7126, Washington, DC 20593-7126, and is available from the sources listed below. (b) American Society of Mechanical Engineers (ASME) International, Three...

  2. 46 CFR 15.915 - Engineer Officer Endorsements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Engineer Officer Endorsements. 15.915 Section 15.915 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Equivalents § 15.915 Engineer Officer Endorsements. The following licenses and MMC officer...

  3. 46 CFR 15.915 - Engineer Officer Endorsements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Engineer Officer Endorsements. 15.915 Section 15.915 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Equivalents § 15.915 Engineer Officer Endorsements. The following licenses and MMC officer...

  4. 46 CFR 15.915 - Engineer Officer Endorsements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Engineer Officer Endorsements. 15.915 Section 15.915 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Equivalents § 15.915 Engineer Officer Endorsements. The following licenses and MMC officer...

  5. 46 CFR 15.915 - Engineer officer endorsements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Engineer officer endorsements. 15.915 Section 15.915 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Equivalents § 15.915 Engineer officer endorsements. The following licenses and MMC officer...

  6. Software Assurance in Acquisition: Mitigating Risks to the Enterprise. A Reference Guide for Security-Enhanced Software Acquisition and Outsourcing

    DTIC Science & Technology

    2009-02-01

    management, available at <http://www.iso.org/ iso /en/CatalogueDetailPage.CatalogueDetail?CSNUMBER=39612&ICS1=35&ICS2=40 &ICS3=>. ISO /IEC 27001 . Information...Management of the Systems Engineering Process. [ ISO /IEC 27001 ] ISO /IEC 27001 :2005. Information technology -- Security techniques -- Information security...software life cycles [ ISO /IEC 15026]. Software assurance is a key element of national security and homeland security. It is critical because dramatic

  7. Cyber security best practices for the nuclear industry

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Badr, I.

    2012-07-01

    When deploying software based systems, such as, digital instrumentation and controls for the nuclear industry, it is vital to include cyber security assessment as part of architecture and development process. When integrating and delivering software-intensive systems for the nuclear industry, engineering teams should make use of a secure, requirements driven, software development life cycle, ensuring security compliance and optimum return on investment. Reliability protections, data loss prevention, and privacy enforcement provide a strong case for installing strict cyber security policies. (authors)

  8. Testing plasmid stability of Escherichia coli using the Continuously Operated Shaken BIOreactor System.

    PubMed

    Sieben, Michaela; Steinhorn, Gregor; Müller, Carsten; Fuchs, Simone; Ann Chin, Laura; Regestein, Lars; Büchs, Jochen

    2016-11-01

    Plasmids are common vectors to genetically manipulate Escherichia coli or other microorganisms. They are easy to use and considerable experience has accumulated on their application in heterologous protein production. However, plasmids can be lost during cell growth, if no selection pressure like, e.g., antibiotics is used, hampering the production of the desired protein and endangering the economic success of a biotechnological production process. Thus, in this study the Continuously Operated Shaken BIOreactor System (COSBIOS) is applied as a tool for fast parallel testing of strain stability and operation conditions and to evaluate measures to counter such plasmid loss. In specific, by applying various ampicillin concentrations, the lowest effective ampicillin dosage is investigated to secure plasmid stability while lowering adverse ecological effects. A significant difference was found in the growth rates of plasmid-bearing and plasmid-free cells. The undesired plasmid-free cells grew 30% faster than the desired plasmid-bearing cells. During the testing of plasmid stability without antibiotics, the population fraction of plasmid-bearing cells rapidly decreased in continuous culture to zero within the first 48 h. An initial single dosage of ampicillin did not prevent plasmid loss. By contrast, a continuous application of a low dosage of 10 µg/mL ampicillin in the feed medium maintained plasmid stability in the culture. Consequently, the COSBIOS is an apt reactor system for measuring plasmid stability and evaluating methods to enhance this stability. Hence, decreased production of heterologous protein can be prevented. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1418-1425, 2016. © 2016 American Institute of Chemical Engineers.

  9. Key Future Engineering Capabilities for Human Capital Retention

    NASA Astrophysics Data System (ADS)

    Sivich, Lorrie

    Projected record retirements of Baby Boomer generation engineers have been predicted to result in significant losses of mission-critical knowledge in space, national security, and future scientific ventures vital to high-technology corporations. No comprehensive review or analysis of engineering capabilities has been performed to identify threats related to the specific loss of mission-critical knowledge posed by the increasing retirement of tenured engineers. Archival data from a single diversified Fortune 500 aerospace manufacturing engineering company's engineering career database were analyzed to ascertain whether relationships linking future engineering capabilities, engineering disciplines, and years of engineering experience could be identified to define critical knowledge transfer models. Chi square, logistic, and linear regression analyses were used to map patterns of discipline-specific, mission-critical knowledge using archival data of engineers' perceptions of engineering capabilities, key developmental experiences, and knowledge learned from their engineering careers. The results from the study were used to document key engineering future capabilities. The results were then used to develop a proposed human capital retention plan to address specific key knowledge gaps of younger engineers as veteran engineers retire. The potential for social change from this study involves informing leaders of aerospace engineering corporations on how to build better quality mentoring or succession plans to fill the void of lost knowledge from retiring engineers. This plan can secure mission-critical knowledge for younger engineers for current and future product development and increased global competitiveness in the technology market.

  10. 46 CFR 167.65-60 - Examination of boilers and machinery by engineer.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Examination of boilers and machinery by engineer. 167.65-60 Section 167.65-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL... machinery by engineer. It shall be the duty of an engineer when he assumes charge of the boilers and...

  11. 46 CFR 167.65-60 - Examination of boilers and machinery by engineer.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Examination of boilers and machinery by engineer. 167.65-60 Section 167.65-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL... machinery by engineer. It shall be the duty of an engineer when he assumes charge of the boilers and...

  12. 46 CFR 167.65-60 - Examination of boilers and machinery by engineer.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Examination of boilers and machinery by engineer. 167.65-60 Section 167.65-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL... machinery by engineer. It shall be the duty of an engineer when he assumes charge of the boilers and...

  13. 46 CFR 167.65-60 - Examination of boilers and machinery by engineer.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Examination of boilers and machinery by engineer. 167.65-60 Section 167.65-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL... machinery by engineer. It shall be the duty of an engineer when he assumes charge of the boilers and...

  14. Agent of opportunity risk mitigation: people, engineering, and security efficacy.

    PubMed

    Graham, Margaret E; Tunik, Michael G; Farmer, Brenna M; Bendzans, Carly; McCrillis, Aileen M; Nelson, Lewis S; Portelli, Ian; Smith, Silas; Goldberg, Judith D; Zhang, Meng; Rosenberg, Sheldon D; Goldfrank, Lewis R

    2010-12-01

    Agents of opportunity (AO) are potentially harmful biological, chemical, radiological, and pharmaceutical substances commonly used for health care delivery and research. AOs are present in all academic medical centers (AMC), creating vulnerability in the health care sector; AO attributes and dissemination methods likely predict risk; and AMCs are inadequately secured against a purposeful AO dissemination, with limited budgets and competing priorities. We explored health care workers' perceptions of AMC security and the impact of those perceptions on AO risk. Qualitative methods (survey, interviews, and workshops) were used to collect opinions from staff working in a medical school and 4 AMC-affiliated hospitals concerning AOs and the risk to hospital infrastructure associated with their uncontrolled presence. Secondary to this goal, staff perception concerning security, or opinions about security behaviors of others, were extracted, analyzed, and grouped into themes. We provide a framework for depicting the interaction of staff behavior and access control engineering, including the tendency of staff to "defeat" inconvenient access controls. In addition, 8 security themes emerged: staff security behavior is a significant source of AO risk; the wide range of opinions about "open" front-door policies among AMC staff illustrates a disparity of perceptions about the need for security; interviewees expressed profound skepticism concerning the effectiveness of front-door access controls; an AO risk assessment requires reconsideration of the security levels historically assigned to areas such as the loading dock and central distribution sites, where many AOs are delivered and may remain unattended for substantial periods of time; researchers' view of AMC security is influenced by the ongoing debate within the scientific community about the wisdom of engaging in bioterrorism research; there was no agreement about which areas of the AMC should be subject to stronger access controls; security personnel play dual roles of security and customer service, creating the negative perception that neither role is done well; and budget was described as an important factor in explaining the state of security controls. We determined that AMCs seeking to reduce AO risk should assess their institutionally unique AO risks, understand staff security perceptions, and install access controls that are responsive to the staff's tendency to defeat them. The development of AO attribute fact sheets is desirable for AO risk assessment; new funding and administrative or legislative tools to improve AMC security are required; and security practices and methods that are convenient and effective should be engineered.

  15. PIA: An Intuitive Protein Inference Engine with a Web-Based User Interface.

    PubMed

    Uszkoreit, Julian; Maerkens, Alexandra; Perez-Riverol, Yasset; Meyer, Helmut E; Marcus, Katrin; Stephan, Christian; Kohlbacher, Oliver; Eisenacher, Martin

    2015-07-02

    Protein inference connects the peptide spectrum matches (PSMs) obtained from database search engines back to proteins, which are typically at the heart of most proteomics studies. Different search engines yield different PSMs and thus different protein lists. Analysis of results from one or multiple search engines is often hampered by different data exchange formats and lack of convenient and intuitive user interfaces. We present PIA, a flexible software suite for combining PSMs from different search engine runs and turning these into consistent results. PIA can be integrated into proteomics data analysis workflows in several ways. A user-friendly graphical user interface can be run either locally or (e.g., for larger core facilities) from a central server. For automated data processing, stand-alone tools are available. PIA implements several established protein inference algorithms and can combine results from different search engines seamlessly. On several benchmark data sets, we show that PIA can identify a larger number of proteins at the same protein FDR when compared to that using inference based on a single search engine. PIA supports the majority of established search engines and data in the mzIdentML standard format. It is implemented in Java and freely available at https://github.com/mpc-bioinformatics/pia.

  16. 40 CFR 91.704 - Exemptions and exclusions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Engines § 91.704 Exemptions and exclusions. (a) Individuals and businesses are eligible to import...) A display marine engine may be imported by any person for purposes related to a business or the... permitted by this paragraph: (1) National security exemption. A nonconforming marine engine may be imported...

  17. 40 CFR 91.704 - Exemptions and exclusions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Engines § 91.704 Exemptions and exclusions. (a) Individuals and businesses are eligible to import...) A display marine engine may be imported by any person for purposes related to a business or the... permitted by this paragraph: (1) National security exemption. A nonconforming marine engine may be imported...

  18. 46 CFR 52.01-1 - Incorporation by reference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther King Jr. Avenue SE., Washington, DC 20593...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990...

  19. 46 CFR 52.01-1 - Incorporation by reference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther King Jr. Avenue SE., Washington, DC 20593...) American Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990...

  20. 46 CFR 63.05-1 - Incorporation by reference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY... is also available for inspection at the U.S. Coast Guard, Office of Design and Engineering Standards.../AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical Engineers (ASME...

  1. 46 CFR 127.620 - Marine engineering requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Marine engineering requirements. 127.620 Section 127.620 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS CONSTRUCTION AND... engineering requirements. Steering gear on OSVs authorized for carriage of more than 240 persons must comply...

  2. CTE's Role in Science, Technology, Engineering and Mathematics

    ERIC Educational Resources Information Center

    Hyslop, Alisha

    2010-01-01

    For the last several years, concern has been brewing about America's underinvestment and underperformance in science, technology, engineering and mathematics--the fields collectively known as STEM. STEM can be described as an initiative for securing America's leadership in science, technology, engineering and mathematics fields and identifying…

  3. Practical and Efficient Searching in Proteomics: A Cross Engine Comparison

    PubMed Central

    Paulo, Joao A.

    2014-01-01

    Background Analysis of large datasets produced by mass spectrometry-based proteomics relies on database search algorithms to sequence peptides and identify proteins. Several such scoring methods are available, each based on different statistical foundations and thereby not producing identical results. Here, the aim is to compare peptide and protein identifications using multiple search engines and examine the additional proteins gained by increasing the number of technical replicate analyses. Methods A HeLa whole cell lysate was analyzed on an Orbitrap mass spectrometer for 10 technical replicates. The data were combined and searched using Mascot, SEQUEST, and Andromeda. Comparisons were made of peptide and protein identifications among the search engines. In addition, searches using each engine were performed with incrementing number of technical replicates. Results The number and identity of peptides and proteins differed across search engines. For all three search engines, the differences in proteins identifications were greater than the differences in peptide identifications indicating that the major source of the disparity may be at the protein inference grouping level. The data also revealed that analysis of 2 technical replicates can increase protein identifications by up to 10-15%, while a third replicate results in an additional 4-5%. Conclusions The data emphasize two practical methods of increasing the robustness of mass spectrometry data analysis. The data show that 1) using multiple search engines can expand the number of identified proteins (union) and validate protein identifications (intersection), and 2) analysis of 2 or 3 technical replicates can substantially expand protein identifications. Moreover, information can be extracted from a dataset by performing database searching with different engines and performing technical repeats, which requires no additional sample preparation and effectively utilizes research time and effort. PMID:25346847

  4. Practical and Efficient Searching in Proteomics: A Cross Engine Comparison.

    PubMed

    Paulo, Joao A

    2013-10-01

    Analysis of large datasets produced by mass spectrometry-based proteomics relies on database search algorithms to sequence peptides and identify proteins. Several such scoring methods are available, each based on different statistical foundations and thereby not producing identical results. Here, the aim is to compare peptide and protein identifications using multiple search engines and examine the additional proteins gained by increasing the number of technical replicate analyses. A HeLa whole cell lysate was analyzed on an Orbitrap mass spectrometer for 10 technical replicates. The data were combined and searched using Mascot, SEQUEST, and Andromeda. Comparisons were made of peptide and protein identifications among the search engines. In addition, searches using each engine were performed with incrementing number of technical replicates. The number and identity of peptides and proteins differed across search engines. For all three search engines, the differences in proteins identifications were greater than the differences in peptide identifications indicating that the major source of the disparity may be at the protein inference grouping level. The data also revealed that analysis of 2 technical replicates can increase protein identifications by up to 10-15%, while a third replicate results in an additional 4-5%. The data emphasize two practical methods of increasing the robustness of mass spectrometry data analysis. The data show that 1) using multiple search engines can expand the number of identified proteins (union) and validate protein identifications (intersection), and 2) analysis of 2 or 3 technical replicates can substantially expand protein identifications. Moreover, information can be extracted from a dataset by performing database searching with different engines and performing technical repeats, which requires no additional sample preparation and effectively utilizes research time and effort.

  5. 78 FR 9951 - Excepted Service

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-12

    ...) Not to exceed 3000 positions that require unique cyber security skills and knowledge to perform cyber..., distributed control systems security, cyber incident response, cyber exercise facilitation and management, cyber vulnerability detection and assessment, network and systems engineering, enterprise architecture...

  6. Crosstalk: The Journal of Defense Software Engineering. Volume 22, Number 3

    DTIC Science & Technology

    2009-04-01

    international standard for information security management systems like ISO /IEC 27001 :2005 [1] existed. Since that time, the organization has developed control...of ISO /IEC 27001 and the desire to make decisions based on business value and risk has prompted Ford’s IT Security and Controls organi- zation to begin...their conventional application security operation.u References 1. ISO /IEC 27001 :2005. “Information Technology – Security Techniques – Information

  7. Atomic force microscopy reveals the mechanical design of a modular protein

    PubMed Central

    Li, Hongbin; Oberhauser, Andres F.; Fowler, Susan B.; Clarke, Jane; Fernandez, Julio M.

    2000-01-01

    Tandem modular proteins underlie the elasticity of natural adhesives, cell adhesion proteins, and muscle proteins. The fundamental unit of elastic proteins is their individually folded modules. Here, we use protein engineering to construct multimodular proteins composed of Ig modules of different mechanical strength. We examine the mechanical properties of the resulting tandem modular proteins by using single protein atomic force microscopy. We show that by combining modules of known mechanical strength, we can generate proteins with novel elastic properties. Our experiments reveal the simple mechanical design of modular proteins and open the way for the engineering of elastic proteins with defined mechanical properties, which can be used in tissue and fiber engineering. PMID:10823913

  8. Atomic force microscopy reveals the mechanical design of a modular protein.

    PubMed

    Li, H; Oberhauser, A F; Fowler, S B; Clarke, J; Fernandez, J M

    2000-06-06

    Tandem modular proteins underlie the elasticity of natural adhesives, cell adhesion proteins, and muscle proteins. The fundamental unit of elastic proteins is their individually folded modules. Here, we use protein engineering to construct multimodular proteins composed of Ig modules of different mechanical strength. We examine the mechanical properties of the resulting tandem modular proteins by using single protein atomic force microscopy. We show that by combining modules of known mechanical strength, we can generate proteins with novel elastic properties. Our experiments reveal the simple mechanical design of modular proteins and open the way for the engineering of elastic proteins with defined mechanical properties, which can be used in tissue and fiber engineering.

  9. Protein Design for Pathway Engineering

    PubMed Central

    Eriksen, Dawn T.; Lian, Jiazhang; Zhao, Huimin

    2013-01-01

    Design and construction of biochemical pathways has increased the complexity of biosynthetically-produced compounds when compared to single enzyme biocatalysis. However, the coordination of multiple enzymes can introduce a complicated set of obstacles to overcome in order to achieve a high titer and yield of the desired compound. Metabolic engineering has made great strides in developing tools to optimize the flux through a target pathway, but the inherent characteristics of a particular enzyme within the pathway can still limit the productivity. Thus, judicious protein design is critical for metabolic and pathway engineering. This review will describe various strategies and examples of applying protein design to pathway engineering to optimize the flux through the pathway. The proteins can be engineered for altered substrate specificity/selectivity, increased catalytic activity, reduced mass transfer limitations through specific protein localization, and reduced substrate/product inhibition. Protein engineering can also be expanded to design biosensors to enable high through-put screening and to customize cell signaling networks. These strategies have successfully engineered pathways for significantly increased productivity of the desired product or in the production of novel compounds. PMID:23558037

  10. Protein design for pathway engineering.

    PubMed

    Eriksen, Dawn T; Lian, Jiazhang; Zhao, Huimin

    2014-02-01

    Design and construction of biochemical pathways has increased the complexity of biosynthetically-produced compounds when compared to single enzyme biocatalysis. However, the coordination of multiple enzymes can introduce a complicated set of obstacles to overcome in order to achieve a high titer and yield of the desired compound. Metabolic engineering has made great strides in developing tools to optimize the flux through a target pathway, but the inherent characteristics of a particular enzyme within the pathway can still limit the productivity. Thus, judicious protein design is critical for metabolic and pathway engineering. This review will describe various strategies and examples of applying protein design to pathway engineering to optimize the flux through the pathway. The proteins can be engineered for altered substrate specificity/selectivity, increased catalytic activity, reduced mass transfer limitations through specific protein localization, and reduced substrate/product inhibition. Protein engineering can also be expanded to design biosensors to enable high through-put screening and to customize cell signaling networks. These strategies have successfully engineered pathways for significantly increased productivity of the desired product or in the production of novel compounds. Copyright © 2013 Elsevier Inc. All rights reserved.

  11. Beyond directed evolution - semi-rational protein engineering and design

    PubMed Central

    Lutz, Stefan

    2010-01-01

    Over the last two decades, directed evolution has transformed the field of protein engineering. The advances in understanding protein structure and function, in no insignificant part a result of directed evolution studies, are increasingly empowering scientists and engineers to device more effective methods for manipulating and tailoring biocatalysts. Abandoning large combinatorial libraries, the focus has shifted to small, functionally-rich libraries and rational design. A critical component to the success of these emerging engineering strategies are computational tools for the evaluation of protein sequence datasets and the analysis of conformational variations of amino acids in proteins. Highlighting the opportunities and limitations of such approaches, this review focuses on recent engineering and design examples that require screening or selection of small libraries. PMID:20869867

  12. Aircraft Engine Exhaust Nozzle System for Jet Noise Reduction

    NASA Technical Reports Server (NTRS)

    Thomas, Russell H. (Inventor); Czech, Michael J. (Inventor); Elkoby, Ronen (Inventor)

    2014-01-01

    The aircraft exhaust engine nozzle system includes a fan nozzle to receive a fan flow from a fan disposed adjacent to an engine disposed above an airframe surface of the aircraft, a core nozzle disposed within the fan nozzle and receiving an engine core flow, and a pylon structure connected to the core nozzle and structurally attached with the airframe surface to secure the engine to the aircraft.

  13. Pre-Engineering Program: Science, Technology, Engineering and Mathematics (STEM)

    DTIC Science & Technology

    2013-08-29

    educators in the Urbana-Champaign area. 15. SUBJECT TERMS STEM: science, technology , engineering, mathematics 16. SECURITY CLASSIFICATION OF: 19a. NAME...9132T-13-1-0002 4. TITLE AND SUBTITLE Pre-Engineering Program: Science, Technology , Engineering and Mathematics (STEM) 5c. PROGRAM ELEMENT NUMBER N...project was focused on underserved children in grades 1-6 who need, but have limited access to, out-of-school time STEM (science, technology

  14. Status of Social Engineering Awareness in Business Organizations and Colleges/Universities

    ERIC Educational Resources Information Center

    Hauser, Deanna Mae

    2017-01-01

    Social engineers manipulate individuals into divulging confidential information or compromising personal or organizational security. The purpose of this qualitative case study was to examine the potential lack of social engineering awareness that affects employees at companies in southeastern Michigan. The research method consisted of interviews…

  15. 40 CFR 90.902 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Exclusion and Exemption of Nonroad Engines from Regulations § 90.902 Definitions. The definitions in subpart A of this part apply to this... exporting new nonroad engines. National security exemption means an exemption granted under § 90.1004(b) for...

  16. 40 CFR 90.902 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Exclusion and Exemption of Nonroad Engines from Regulations § 90.902 Definitions. The definitions in subpart A of this part apply to this... exporting new nonroad engines. National security exemption means an exemption granted under § 90.1004(b) for...

  17. 40 CFR 90.902 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Exclusion and Exemption of Nonroad Engines from Regulations § 90.902 Definitions. The definitions in subpart A of this part apply to this... exporting new nonroad engines. National security exemption means an exemption granted under § 90.1004(b) for...

  18. 40 CFR 90.902 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Exclusion and Exemption of Nonroad Engines from Regulations § 90.902 Definitions. The definitions in subpart A of this part apply to this... exporting new nonroad engines. National security exemption means an exemption granted under § 90.1004(b) for...

  19. 46 CFR 113.35-13 - Mechanical engine order telegraph systems; operation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Mechanical engine order telegraph systems; operation. 113.35-13 Section 113.35-13 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED...-13 Mechanical engine order telegraph systems; operation. If more than one transmitter operates a...

  20. 46 CFR 15.820 - Chief engineer.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Chief engineer. 15.820 Section 15.820 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.820 Chief engineer. (a) There must be an individual holding an MMC or license endorsed as...

  1. 46 CFR 52.01-1 - Incorporation by reference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... inspection at the U.S. Coast Guard, Office of Design and Engineering Standards (CG-ENG), 2100 2nd St., SW... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001...

  2. 46 CFR 63.05-1 - Incorporation by reference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY... is also available for inspection at the U.S. Coast Guard, Office of Design and Engineering Standards... (“ANSI/AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical Engineers (ASME...

  3. 46 CFR 52.01-1 - Incorporation by reference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... inspection at the U.S. Coast Guard, Office of Design and Engineering Standards (CG-521), 2100 2nd St., SW... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001...

  4. 46 CFR 52.01-1 - Incorporation by reference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING POWER BOILERS General... inspection at the U.S. Coast Guard, Office of Design and Engineering Standards (CG-521), 2100 2nd St., SW... Society of Mechanical Engineers (ASME) International, Three Park Avenue, New York, NY 10016-5990: (1) 2001...

  5. 46 CFR 63.05-1 - Incorporation by reference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY... is also available for inspection at the U.S. Coast Guard, Office of Design and Engineering Standards... (“ANSI/AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical Engineers (ASME...

  6. 46 CFR 15.820 - Chief engineer.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Chief engineer. 15.820 Section 15.820 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.820 Chief engineer. (a) There must be an individual holding an MMC or license endorsed as...

  7. 46 CFR 15.820 - Chief engineer.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Chief engineer. 15.820 Section 15.820 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.820 Chief engineer. (a) There must be an individual holding an MMC or license endorsed as...

  8. 46 CFR 15.820 - Chief engineer.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Chief engineer. 15.820 Section 15.820 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.820 Chief engineer. (a) There must be an individual holding an MMC or license endorsed as...

  9. 46 CFR 15.820 - Chief engineer.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Chief engineer. 15.820 Section 15.820 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.820 Chief engineer. (a) There must be an individual holding an MMC or license endorsed as...

  10. Information engineering

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hunt, D.N.

    1997-02-01

    The Information Engineering thrust area develops information technology to support the programmatic needs of Lawrence Livermore National Laboratory`s Engineering Directorate. Progress in five programmatic areas are described in separate reports contained herein. These are entitled Three-dimensional Object Creation, Manipulation, and Transport, Zephyr:A Secure Internet-Based Process to Streamline Engineering Procurements, Subcarrier Multiplexing: Optical Network Demonstrations, Parallel Optical Interconnect Technology Demonstration, and Intelligent Automation Architecture.

  11. 46 CFR 11.514 - Service requirements for second assistant engineer of steam and/or motor vessels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... steam and/or motor vessels. 11.514 Section 11.514 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Engineer Officer § 11.514 Service requirements for second assistant engineer of steam and/or motor vessels... steam and/or motor vessels is: (a) One year of service as an assistant engineer, while holding a license...

  12. 46 CFR 11.524 - Service requirements for designated duty engineer of steam and/or motor vessels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... steam and/or motor vessels. 11.524 Section 11.524 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Engineer Officer § 11.524 Service requirements for designated duty engineer of steam and/or motor vessels... requirements for endorsements as DDE are: (1) For designated duty engineer of steam and/or motor vessels of any...

  13. 46 CFR 11.512 - Service requirements for first assistant engineer of steam and/or motor vessels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... steam and/or motor vessels. 11.512 Section 11.512 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Engineer Officer § 11.512 Service requirements for first assistant engineer of steam and/or motor vessels... steam and/or motor vessels is one year of service as an assistant engineer, while holding a license or...

  14. EPA Science Matters Newsletter: Advancing Ways to Clean Up Drinking Water Systems (Published November 2013)

    EPA Pesticide Factsheets

    To advance the science and engineering of decontaminating pipe systems and safely disposing of high-volumes of contaminated water, Agency homeland security researchers are developing a Water Security Test Bed (WSTB).

  15. 48 CFR - Unknown Title

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Section Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND SECURITY ACQUISITION REGULATION (HSAR) SPECIAL CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACTS Special Aspects of Contracting for Constructio...

  16. 48 CFR - Unknown Title

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Section Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND SECURITY ACQUISITION REGULATION (HSAR) SPECIAL CATEGORIES OF CONTRACTING CONSTRUCTION AND ARCHITECT-ENGINEER CONTRACTS Special Aspects of Contracting for Constructio...

  17. IntegromeDB: an integrated system and biological search engine.

    PubMed

    Baitaluk, Michael; Kozhenkov, Sergey; Dubinina, Yulia; Ponomarenko, Julia

    2012-01-19

    With the growth of biological data in volume and heterogeneity, web search engines become key tools for researchers. However, general-purpose search engines are not specialized for the search of biological data. Here, we present an approach at developing a biological web search engine based on the Semantic Web technologies and demonstrate its implementation for retrieving gene- and protein-centered knowledge. The engine is available at http://www.integromedb.org. The IntegromeDB search engine allows scanning data on gene regulation, gene expression, protein-protein interactions, pathways, metagenomics, mutations, diseases, and other gene- and protein-related data that are automatically retrieved from publicly available databases and web pages using biological ontologies. To perfect the resource design and usability, we welcome and encourage community feedback.

  18. A novel quantum scheme for secure two-party distance computation

    NASA Astrophysics Data System (ADS)

    Peng, Zhen-wan; Shi, Run-hua; Zhong, Hong; Cui, Jie; Zhang, Shun

    2017-12-01

    Secure multiparty computational geometry is an essential field of secure multiparty computation, which computes a computation geometric problem without revealing any private information of each party. Secure two-party distance computation is a primitive of secure multiparty computational geometry, which computes the distance between two points without revealing each point's location information (i.e., coordinate). Secure two-party distance computation has potential applications with high secure requirements in military, business, engineering and so on. In this paper, we present a quantum solution to secure two-party distance computation by subtly using quantum private query. Compared to the classical related protocols, our quantum protocol can ensure higher security and better privacy protection because of the physical principle of quantum mechanics.

  19. An ontology-based search engine for protein-protein interactions

    PubMed Central

    2010-01-01

    Background Keyword matching or ID matching is the most common searching method in a large database of protein-protein interactions. They are purely syntactic methods, and retrieve the records in the database that contain a keyword or ID specified in a query. Such syntactic search methods often retrieve too few search results or no results despite many potential matches present in the database. Results We have developed a new method for representing protein-protein interactions and the Gene Ontology (GO) using modified Gödel numbers. This representation is hidden from users but enables a search engine using the representation to efficiently search protein-protein interactions in a biologically meaningful way. Given a query protein with optional search conditions expressed in one or more GO terms, the search engine finds all the interaction partners of the query protein by unique prime factorization of the modified Gödel numbers representing the query protein and the search conditions. Conclusion Representing the biological relations of proteins and their GO annotations by modified Gödel numbers makes a search engine efficiently find all protein-protein interactions by prime factorization of the numbers. Keyword matching or ID matching search methods often miss the interactions involving a protein that has no explicit annotations matching the search condition, but our search engine retrieves such interactions as well if they satisfy the search condition with a more specific term in the ontology. PMID:20122195

  20. An ontology-based search engine for protein-protein interactions.

    PubMed

    Park, Byungkyu; Han, Kyungsook

    2010-01-18

    Keyword matching or ID matching is the most common searching method in a large database of protein-protein interactions. They are purely syntactic methods, and retrieve the records in the database that contain a keyword or ID specified in a query. Such syntactic search methods often retrieve too few search results or no results despite many potential matches present in the database. We have developed a new method for representing protein-protein interactions and the Gene Ontology (GO) using modified Gödel numbers. This representation is hidden from users but enables a search engine using the representation to efficiently search protein-protein interactions in a biologically meaningful way. Given a query protein with optional search conditions expressed in one or more GO terms, the search engine finds all the interaction partners of the query protein by unique prime factorization of the modified Gödel numbers representing the query protein and the search conditions. Representing the biological relations of proteins and their GO annotations by modified Gödel numbers makes a search engine efficiently find all protein-protein interactions by prime factorization of the numbers. Keyword matching or ID matching search methods often miss the interactions involving a protein that has no explicit annotations matching the search condition, but our search engine retrieves such interactions as well if they satisfy the search condition with a more specific term in the ontology.

  1. VisANT 3.0: new modules for pathway visualization, editing, prediction and construction.

    PubMed

    Hu, Zhenjun; Ng, David M; Yamada, Takuji; Chen, Chunnuan; Kawashima, Shuichi; Mellor, Joe; Linghu, Bolan; Kanehisa, Minoru; Stuart, Joshua M; DeLisi, Charles

    2007-07-01

    With the integration of the KEGG and Predictome databases as well as two search engines for coexpressed genes/proteins using data sets obtained from the Stanford Microarray Database (SMD) and Gene Expression Omnibus (GEO) database, VisANT 3.0 supports exploratory pathway analysis, which includes multi-scale visualization of multiple pathways, editing and annotating pathways using a KEGG compatible visual notation and visualization of expression data in the context of pathways. Expression levels are represented either by color intensity or by nodes with an embedded expression profile. Multiple experiments can be navigated or animated. Known KEGG pathways can be enriched by querying either coexpressed components of known pathway members or proteins with known physical interactions. Predicted pathways for genes/proteins with unknown functions can be inferred from coexpression or physical interaction data. Pathways produced in VisANT can be saved as computer-readable XML format (VisML), graphic images or high-resolution Scalable Vector Graphics (SVG). Pathways in the format of VisML can be securely shared within an interested group or published online using a simple Web link. VisANT is freely available at http://visant.bu.edu.

  2. Engineering Software for Interoperability through Use of Enterprise Architecture Techniques

    DTIC Science & Technology

    2003-03-01

    Response Home/ Business Security . To detect flood conditions (i.e. excess water levels) within the monitored area and alert authorities, as necessary...Response; Fire Detection & Response; and Flood Detection & Response. Functional Area Description Intruder Detection & Response Home/ Business ... Security . To monitor and detect unauthorized entry into the secured area and sound alarms/alert authorities, as necessary. Fire Detection

  3. An Evaluation Methodology for the Usability and Security of Cloud-based File Sharing Technologies

    DTIC Science & Technology

    2012-09-01

    FISMA, ISO 27001 , FIPS 140-2, and ISO 270001) indicate a cloud-based service’s compliance with industry standard security controls, management and...Information Assurance IEEE Institute of Electrical and Electronics Engineers IT Information Technology ITS Insider Threat Study ISO International...effectively, efficiently and with satisfaction” (International Organization for Standardization [ ISO ], 1998). Alternately, information security

  4. A study on an information security system of a regional collaborative medical platform.

    PubMed

    Zhao, Junping; Peng, Kun; Leng, Jinchang; Sun, Xiaowei; Zhang, Zhenjiang; Xue, Wanguo; Ren, Lianzhong

    2010-01-01

    The objective of this study was to share the experience of building an information security system for a regional collaborative medical platform (RCMP) and discuss the lessons learned from practical projects. Safety measures are analyzed from the perspective of system engineering. We present the essential requirements, critical architectures, and policies for system security of regional collaborative medical platforms.

  5. Introduction to current and future protein therapeutics: a protein engineering perspective.

    PubMed

    Carter, Paul J

    2011-05-15

    Protein therapeutics and its enabling sister discipline, protein engineering, have emerged since the early 1980s. The first protein therapeutics were recombinant versions of natural proteins. Proteins purposefully modified to increase their clinical potential soon followed with enhancements derived from protein or glycoengineering, Fc fusion or conjugation to polyethylene glycol. Antibody-based drugs subsequently arose as the largest and fastest growing class of protein therapeutics. The rationale for developing better protein therapeutics with enhanced efficacy, greater safety, reduced immunogenicity or improved delivery comes from the convergence of clinical, scientific, technological and commercial drivers that have identified unmet needs and provided strategies to address them. Future protein drugs seem likely to be more extensively engineered to improve their performance, e.g., antibodies and Fc fusion proteins with enhanced effector functions or extended half-life. Two old concepts for improving antibodies, namely antibody-drug conjugates and bispecific antibodies, have advanced to the cusp of clinical success. As for newer protein therapeutic platform technologies, several engineered protein scaffolds are in early clinical development and offer differences and some potential advantages over antibodies. Copyright © 2011 Elsevier Inc. All rights reserved.

  6. Introduction to current and future protein therapeutics: A protein engineering perspective

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Carter, Paul J., E-mail: pjc@gene.com

    2011-05-15

    Protein therapeutics and its enabling sister discipline, protein engineering, have emerged since the early 1980s. The first protein therapeutics were recombinant versions of natural proteins. Proteins purposefully modified to increase their clinical potential soon followed with enhancements derived from protein or glycoengineering, Fc fusion or conjugation to polyethylene glycol. Antibody-based drugs subsequently arose as the largest and fastest growing class of protein therapeutics. The rationale for developing better protein therapeutics with enhanced efficacy, greater safety, reduced immunogenicity or improved delivery comes from the convergence of clinical, scientific, technological and commercial drivers that have identified unmet needs and provided strategies tomore » address them. Future protein drugs seem likely to be more extensively engineered to improve their performance, e.g., antibodies and Fc fusion proteins with enhanced effector functions or extended half-life. Two old concepts for improving antibodies, namely antibody-drug conjugates and bispecific antibodies, have advanced to the cusp of clinical success. As for newer protein therapeutic platform technologies, several engineered protein scaffolds are in early clinical development and offer differences and some potential advantages over antibodies.« less

  7. A Formal Specification and Verification Method for the Prevention of Denial of Service in Ada Services

    DTIC Science & Technology

    1988-03-01

    Mechanism; Computer Security. 16. PRICE CODE 17. SECURITY CLASSIFICATION IS. SECURITY CLASSIFICATION 19. SECURITY CLASSIFICATION 20. UMrrATION OF ABSTRACT...denial of service. This paper assumes that the reader is a computer science or engineering professional working in the area of formal specification and...recovery from such events as deadlocks and crashes can be accounted for in the computation of the waiting time for each service in the service hierarchy

  8. Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins

    PubMed Central

    Polstein, Lauren R.; Gersbach, Charles A.

    2014-01-01

    The coupling of light-inducible protein-protein interactions with gene regulation systems has enabled the control of gene expression with light. In particular, heterodimer protein pairs from plants can be used to engineer a gene regulation system in mammalian cells that is reversible, repeatable, tunable, controllable in a spatiotemporal manner, and targetable to any DNA sequence. This system, Light-Inducible Transcription using Engineered Zinc finger proteins (LITEZ), is based on the blue light-induced interaction of GIGANTEA and the LOV domain of FKF1 that drives the localization of a transcriptional activator to the DNA-binding site of a highly customizable engineered zinc finger protein. This chapter provides methods for modifying LITEZ to target new DNA sequences, engineering a programmable LED array to illuminate cell cultures, and using the modified LITEZ system to achieve spatiotemporal control of transgene expression in mammalian cells. PMID:24718797

  9. Fn3 proteins engineered to recognize tumor biomarker mesothelin internalize upon binding

    PubMed Central

    Sirois, Allison R.; Deny, Daniela A.; Baierl, Samantha R.; George, Katia S.

    2018-01-01

    Mesothelin is a cell surface protein that is overexpressed in numerous cancers, including breast, ovarian, lung, liver, and pancreatic tumors. Aberrant expression of mesothelin has been shown to promote tumor progression and metastasis through interaction with established tumor biomarker CA125. Therefore, molecules that specifically bind to mesothelin have potential therapeutic and diagnostic applications. However, no mesothelin-targeting molecules are currently approved for routine clinical use. While antibodies that target mesothelin are in development, some clinical applications may require a targeting molecule with an alternative protein fold. For example, non-antibody proteins are more suitable for molecular imaging and may facilitate diverse chemical conjugation strategies to create drug delivery complexes. In this work, we engineered variants of the fibronectin type III domain (Fn3) non-antibody protein scaffold to bind to mesothelin with high affinity, using directed evolution and yeast surface display. Lead engineered Fn3 variants were solubly produced and purified from bacterial culture at high yield. Upon specific binding to mesothelin on human cancer cell lines, the engineered Fn3 proteins internalized and co-localized to early endosomes. To our knowledge, this is the first report of non-antibody proteins engineered to bind mesothelin. The results validate that non-antibody proteins can be engineered to bind to tumor biomarker mesothelin, and encourage the continued development of engineered variants for applications such as targeted diagnostics and therapeutics. PMID:29738555

  10. Home - Defense Technology Security Administration

    Science.gov Websites

    , engineering, and math (STEM) focused Girl Scout troops 2210 and 5064 into the Mark Center to showcase their to welcome science, technology, engineering, and math (STEM) focused Girl Scout troops 2210 and 5064

  11. 77 FR 36292 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-Border Security...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ... research, development, engineering and production of border security-related systems; and (iii) to insert.... Patricia A. Brink, Director of Civil Enforcement, Antitrust Division. [FR Doc. 2012-14766 Filed 6-15-12; 8...

  12. Deep sequencing methods for protein engineering and design.

    PubMed

    Wrenbeck, Emily E; Faber, Matthew S; Whitehead, Timothy A

    2017-08-01

    The advent of next-generation sequencing (NGS) has revolutionized protein science, and the development of complementary methods enabling NGS-driven protein engineering have followed. In general, these experiments address the functional consequences of thousands of protein variants in a massively parallel manner using genotype-phenotype linked high-throughput functional screens followed by DNA counting via deep sequencing. We highlight the use of information rich datasets to engineer protein molecular recognition. Examples include the creation of multiple dual-affinity Fabs targeting structurally dissimilar epitopes and engineering of a broad germline-targeted anti-HIV-1 immunogen. Additionally, we highlight the generation of enzyme fitness landscapes for conducting fundamental studies of protein behavior and evolution. We conclude with discussion of technological advances. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Addressing social resistance in emerging security technologies

    PubMed Central

    Mitchener-Nissen, Timothy

    2013-01-01

    In their efforts to enhance the safety and security of citizens, governments and law enforcement agencies look to scientists and engineers to produce modern methods for preventing, detecting, and prosecuting criminal activities. Whole body scanners, lie detection technologies, biometrics, etc., are all being developed for incorporation into the criminal justice apparatus.1 Yet despite their purported security benefits these technologies often evoke social resistance. Concerns over privacy, ethics, and function-creep appear repeatedly in analyses of these technologies. It is argued here that scientists and engineers continue to pay insufficient attention to this resistance; acknowledging the presence of these social concerns yet failing to meaningfully address them. In so doing they place at risk the very technologies and techniques they are seeking to develop, for socially controversial security technologies face restrictions and in some cases outright banning. By identifying sources of potential social resistance early in the research and design process, scientists can both engage with the public in meaningful debate and modify their security technologies before deployment so as to minimize social resistance and enhance uptake. PMID:23970863

  14. 76 FR 13667 - Chrysler Group LLC; Formerly Known as Chrysler LLC; Kenosha Engine Plant; Including On-Site...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ...; Formerly Known as Chrysler LLC; Kenosha Engine Plant; Including On-Site Leased Workers From Caravan Knight..., K+S Services, Inc., G4S Secure Solutions, Crassociates, Inc., CES, INC., Evans Distribution Systems... of Chrysler Group, LLC, formerly known as Chrysler, LLC, Kenosha Engine Plant, Kenosha, Wisconsin...

  15. 40 CFR 91.1004 - Who may request an exemption.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (CONTINUED) CONTROL OF EMISSIONS FROM MARINE SPARK-IGNITION ENGINES Exclusion and Exemption of Marine SI Engines § 91.1004 Who may request an exemption. (a) Any person may request a testing exemption under § 91.1005. (b) Any marine SI engine manufacturer may request a national security exemption under § 91.1008...

  16. 46 CFR 32.35-5 - Installation of internal combustion engines-TB/ALL.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Installation of internal combustion engines-TB/ALL. 32.35-5 Section 32.35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL... combustion engines—TB/ALL. Each internal combustion engine located on the weather deck shall be provided with...

  17. 46 CFR 32.35-5 - Installation of internal combustion engines-TB/ALL.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Installation of internal combustion engines-TB/ALL. 32.35-5 Section 32.35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL... combustion engines—TB/ALL. Each internal combustion engine located on the weather deck shall be provided with...

  18. 46 CFR 32.35-5 - Installation of internal combustion engines-TB/ALL.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Installation of internal combustion engines-TB/ALL. 32.35-5 Section 32.35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL... combustion engines—TB/ALL. Each internal combustion engine located on the weather deck shall be provided with...

  19. 46 CFR 32.35-5 - Installation of internal combustion engines-TB/ALL.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Installation of internal combustion engines-TB/ALL. 32.35-5 Section 32.35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL... combustion engines—TB/ALL. Each internal combustion engine located on the weather deck shall be provided with...

  20. 46 CFR 32.35-5 - Installation of internal combustion engines-TB/ALL.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Installation of internal combustion engines-TB/ALL. 32.35-5 Section 32.35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY TANK VESSELS SPECIAL... combustion engines—TB/ALL. Each internal combustion engine located on the weather deck shall be provided with...

  1. 46 CFR 15.865 - Qualified member of the engine department (QMED).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Qualified member of the engine department (QMED). 15.865 Section 15.865 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MANNING REQUIREMENTS Computations § 15.865 Qualified member of the engine department (QMED). (a) Every...

  2. PLUMBBOB Series, 1957

    DTIC Science & Technology

    1981-09-15

    Organization included radiological safety, security, transportation, cominuni- cations, engineering , and logistics. The Air Force Special Weapons Center (AFSWC...Test Organization by Reynolds Electrical and Engineering Company. Inc. * PLUMBBOB AFSWP Operation Summary Report * Weapons Test Reports for the Armed...Project 50.5 (Evaluation of’ Shielding for Engineer ileavy Equipment) .... ......... . 4.2.4 Project 50.6 (Protection Afforded by "Field

  3. An Evaluation of the Pavement Condition Index Prediction Model for Rigid Airfield Pavements

    DTIC Science & Technology

    1982-09-01

    UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGO(I*%A Data Entotoi) The United States Army Corps of Engineers, Construction Engineering Research Laboratory...Condition . . . 31 Pavement Design/ Construction ....... . 82 Aircraft Traffic ........ .............. 82 Climate Conditions ........ ............. 84...PATTERSON AFB . . . . . . . . . . . . . . . . . . . . . . . . . 155 C. DATA OBTAINED FROM THE CONSTRUCTION ENGINEERING RESEARCH LABORATORY. .. .. 168 D

  4. Measuring the Immeasurable: An Approach to Assessing the Effectiveness of Engineering Civic Assistance Projects Towards Achieving National Security Objectives

    DTIC Science & Technology

    2015-06-12

    MEASURING THE IMMEASURABLE: AN APPROACH TO ASSESSING THE EFFECTIVENESS OF ENGINEERING CIVIC ASSISTANCE PROJECTS TOWARDS ACHIEVING...SUBTITLE Measuring the Immeasurable: An Approach to Assessing the Effectiveness of Engineering Civic Assistance Projects Towards Achieving National...increasing reliance on Humanitarian and Civic Assistance (HCA), specifically engineering civic assistance projects (ENCAPs), as a way to shape the

  5. NREL Fuels and Engines R&D Revs Up Vehicle Efficiency, Performance

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    NREL bridges fuels and engines R&D to maximize vehicle efficiency and performance. The lab’s fuels and engines research covers the full spectrum of innovation—from fuel chemistry, conversion, and combustion to the evaluation of how fuels interact with engine and vehicle design. This innovative approach has the potential to positively impact our economy, national energy security, and air quality.

  6. An Integrated Approach for Physical and Cyber Security Risk Assessment: The U.S. Army Corps of Engineers Common Risk Model for Dams

    DTIC Science & Technology

    2016-07-01

    Common Risk Model for Dams ( CRM -D) Methodology,” for the Director, Cost Assessment and Program Evaluation, Office of Secretary of Defense and the...for Dams ( CRM -D), developed by the U.S. Army Corps of Engineers (USACE) in collaboration with the Institute for Defense Analyses (IDA) and the U.S...and cyber security risks across a portfolio of dams, and informing decisions on how to mitigate those risks. The CRM -D can effectively quantify the

  7. IntegromeDB: an integrated system and biological search engine

    PubMed Central

    2012-01-01

    Background With the growth of biological data in volume and heterogeneity, web search engines become key tools for researchers. However, general-purpose search engines are not specialized for the search of biological data. Description Here, we present an approach at developing a biological web search engine based on the Semantic Web technologies and demonstrate its implementation for retrieving gene- and protein-centered knowledge. The engine is available at http://www.integromedb.org. Conclusions The IntegromeDB search engine allows scanning data on gene regulation, gene expression, protein-protein interactions, pathways, metagenomics, mutations, diseases, and other gene- and protein-related data that are automatically retrieved from publicly available databases and web pages using biological ontologies. To perfect the resource design and usability, we welcome and encourage community feedback. PMID:22260095

  8. Protein-based hydrogels for tissue engineering

    PubMed Central

    Schloss, Ashley C.; Williams, Danielle M.; Regan, Lynne J.

    2017-01-01

    The tunable mechanical and structural properties of protein-based hydrogels make them excellent scaffolds for tissue engineering and repair. Moreover, using protein-based components provides the option to insert sequences associated with the promoting both cellular adhesion to the substrate and overall cell growth. Protein-based hydrogel components are appealing for their structural designability, specific biological functionality, and stimuli-responsiveness. Here we present highlights in the field of protein-based hydrogels for tissue engineering applications including design requirements, components, and gel types. PMID:27677513

  9. Laboratory Directed Research and Development FY2010 Annual Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jackson, K J

    2011-03-22

    A premier applied-science laboratory, Lawrence Livermore National Laboratory (LLNL) has at its core a primary national security mission - to ensure the safety, security, and reliability of the nation's nuclear weapons stockpile without nuclear testing, and to prevent and counter the spread and use of weapons of mass destruction: nuclear, chemical, and biological. The Laboratory uses the scientific and engineering expertise and facilities developed for its primary mission to pursue advanced technologies to meet other important national security needs - homeland defense, military operations, and missile defense, for example - that evolve in response to emerging threats. For broader nationalmore » needs, LLNL executes programs in energy security, climate change and long-term energy needs, environmental assessment and management, bioscience and technology to improve human health, and for breakthroughs in fundamental science and technology. With this multidisciplinary expertise, the Laboratory serves as a science and technology resource to the U.S. government and as a partner with industry and academia. This annual report discusses the following topics: (1) Advanced Sensors and Instrumentation; (2) Biological Sciences; (3) Chemistry; (4) Earth and Space Sciences; (5) Energy Supply and Use; (6) Engineering and Manufacturing Processes; (7) Materials Science and Technology; Mathematics and Computing Science; (8) Nuclear Science and Engineering; and (9) Physics.« less

  10. Meet EPA Engineer Shawn Ryan, Ph.D.

    EPA Pesticide Factsheets

    Shawn Ryan, Ph.D. is a chemical engineer at EPA's National Homeland Security Research Center. He has worked at EPA for 12 years, nine of which have been devoted to leading research to support decontamination and consequence management.

  11. Los Alamos National Security, LLC Request for Information from industrial entities that desire to commercialize Laboratory-developed Extremely Low Resource Optical Identifier (ELROI) tech

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Erickson, Michael Charles

    Los Alamos National Security, LLC (LANS) is the manager and operator of the Los Alamos National Laboratory for the U.S. Department of Energy National Nuclear Security Administration under contract DE-AC52-06NA25396. LANS is a mission-centric Federally Funded Research and Development Center focused on solving the most critical national security challenges through science and engineering for both government and private customers.

  12. A System Architecture to Support a Verifiably Secure Multilevel Security System.

    DTIC Science & Technology

    1980-06-01

    4] Newmann, P.G., R. Fabry, K. Levitt, L. Robin - provide a tradeoff between cost and system secur- son, J. Wensley , "On the Design of a Provably ity...ICS-80/05 NL 112. 11W1 --1.25 1111 6 Mli,’O~ll Rl OIIION W AII .q3 0 School of Information and Computer Science S =GEORGIA INSTITUTE OF TECHNOLOGY 808...Multilevel Security Systemt (Extended Abstract) George I. Davida Department of Electical Engineering and Computer Science University of Wisconsin

  13. Engineered Proteins: Redox Properties and Their Applications

    PubMed Central

    Prabhulkar, Shradha; Tian, Hui; Wang, Xiaotang; Zhu, Jun-Jie

    2012-01-01

    Abstract Oxidoreductases and metalloproteins, representing more than one third of all known proteins, serve as significant catalysts for numerous biological processes that involve electron transfers such as photosynthesis, respiration, metabolism, and molecular signaling. The functional properties of the oxidoreductases/metalloproteins are determined by the nature of their redox centers. Protein engineering is a powerful approach that is used to incorporate biological and abiological redox cofactors as well as novel enzymes and redox proteins with predictable structures and desirable functions for important biological and chemical applications. The methods of protein engineering, mainly rational design, directed evolution, protein surface modifications, and domain shuffling, have allowed the creation and study of a number of redox proteins. This review presents a selection of engineered redox proteins achieved through these methods, resulting in a manipulation in redox potentials, an increase in electron-transfer efficiency, and an expansion of native proteins by de novo design. Such engineered/modified redox proteins with desired properties have led to a broad spectrum of practical applications, ranging from biosensors, biofuel cells, to pharmaceuticals and hybrid catalysis. Glucose biosensors are one of the most successful products in enzyme electrochemistry, with reconstituted glucose oxidase achieving effective electrical communication with the sensor electrode; direct electron-transfer-type biofuel cells are developed to avoid thermodynamic loss and mediator leakage; and fusion proteins of P450s and redox partners make the biocatalytic generation of drug metabolites possible. In summary, this review includes the properties and applications of the engineered redox proteins as well as their significance and great potential in the exploration of bioelectrochemical sensing devices. Antioxid. Redox Signal. 17, 1796–1822. PMID:22435347

  14. A&M. Radioactive parts security storage area. camera facing northwest. Outdoor ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    A&M. Radioactive parts security storage area. camera facing northwest. Outdoor storage of concrete storage casks. Photographer: M. Holmes. Date: November 21, 1959. INEEL negative no. 59-6081 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  15. Systemic Vulnerabilities

    DTIC Science & Technology

    2014-10-01

    CRm CAL FA~WR£S Q I • Software Engineering Institute I Ccamt>gw l\\~llon Lniwndty 34 Basic attack tree Destroy Building Generate Sufficient...by computer-security company marketing literature that touts 11hacker proof software,11 11triple-DES security,11 and the like. In truth, unbreakable

  16. Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris.

    PubMed

    Prabhu, Ashish A; Boro, Bibari; Bharali, Biju; Chakraborty, Shuchishloka; Dasu, Veeranki V

    2017-01-01

    Process development involving system metabolic engineering and bioprocess engineering has become one of the major thrust for the development of therapeutic proteins or enzymes. Pichia pastoris has emerged as a prominent host for the production of therapeutic protein or enzymes. Regardless of producing high protein titers, various cellular and process level bottlenecks restrict the expression of recombinant proteins in P. pastoris. In the present review, we have summarized the recent developments in the expression of foreign proteins in P. pastoris. Further, we have discussed various cellular engineering strategies which include codon optimization, pathway engineering, signal peptide processing, development of protease deficient strain and glyco-engineered strains for the high yield protein secretion of recombinant protein. Bioprocess development of recombinant proteins in large-scale bioreactor including medium optimization, optimum feeding strategy and co-substrate feeding in fed-batch as well as continuous cultivation have been described. The recent advances in system and synthetic biology studies including metabolic flux analysis in understanding the phenotypic characteristics of recombinant Pichia and genome editing with CRISPR-CAS system have also been summarized. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  17. CrossTalk. The Journal of Defense Software Engineering. Volume 25, Number 6

    DTIC Science & Technology

    2012-12-01

    Cyber Security Threat Definition Communicable Noncommunicable Based on Risky Behavior Coordinated Trojan horse programs Threats hidden in a...for Cyber Security Threats Cyber Security Threat Communicable Noncommunicable Risky Behaviors Coordinated Type of Intervention (at the System...types of data are breached. Further, educational materials on risky behaviors (e.g., for home Internet users) as well as recommended guide- lines for

  18. Foundations for Security Aware Software Development Education

    DTIC Science & Technology

    2005-11-22

    depending on the budget, that support robustness. We discuss the educational customer base, projected lifetime, and complexity of paradigm shift that should...in Honour of Sir Tony Hoar, [6] Cheetham, C. and Ferraiolo, K., "The Systems Security Millenial Perspectives in Computer Science, Engineering...Capability Maturity Model", 21st 2002, 229-246. National Information Systems Security Conference, [15] Schwartz, J., "Object Oriented Extensions to October 5

  19. Study of Software Tools to Support Systems Engineering Management

    DTIC Science & Technology

    2015-06-01

    Management 15. NUMBER OF PAGES 137 16. PRICE CODE 17. SECURITY CLASSIFICATION OF REPORT Unclassified 18. SECURITY CLASSIFICATION OF THIS...AVAILABILITY STATEMENT Approved for public release; distribution is unlimited 12b. DISTRIBUTION CODE 13. ABSTRACT (maximum 200 words) According to a...PAGE Unclassified 19. SECURITY CLASSIFICATION OF ABSTRACT Unclassified 20. LIMITATION OF ABSTRACT UU NSN 7540–01–280–5500 Standard Form 298

  20. Finding the ’RITE’ Acquisition Environment for Navy C2 Software

    DTIC Science & Technology

    2015-05-01

    Boiler plate contract language - Gov purpose Rights • Adding expectation of quality to contracting language • Template SOW’s created Pr...Debugger MCCABE IQ Static Analysis Cyclomatic Complexity and KSLOC. All Languages HP Fortify Security Scan STIG and Vulnerabilities Security & IA...GSSAT (GOTS) Security Scan STIG and Vulnerabilities AutoIT Automated Test Scripting Engine for Automation Functional Testing TestComplete Automated

  1. American Security and the International Energy Situation. Volume 4. Collected Papers

    DTIC Science & Technology

    1975-04-15

    piograms as jet engine sales Wheat shipments may permit the Soviets to keep chemical industries onenled l.siim.«,, ,1 Pi.l.vs.., I...security and economic interde- pendence among Western advanced industrialized countries. Periodic con- flicts have been replaced by a "security... industrialized countries, creating an "interpenetration of econ- omies." Each development affects the dimensions of the access-to- resources

  2. Reverse and forward engineering of protein pattern formation.

    PubMed

    Kretschmer, Simon; Harrington, Leon; Schwille, Petra

    2018-05-26

    Living systems employ protein pattern formation to regulate important life processes in space and time. Although pattern-forming protein networks have been identified in various prokaryotes and eukaryotes, their systematic experimental characterization is challenging owing to the complex environment of living cells. In turn, cell-free systems are ideally suited for this goal, as they offer defined molecular environments that can be precisely controlled and manipulated. Towards revealing the molecular basis of protein pattern formation, we outline two complementary approaches: the biochemical reverse engineering of reconstituted networks and the de novo design, or forward engineering, of artificial self-organizing systems. We first illustrate the reverse engineering approach by the example of the Escherichia coli Min system, a model system for protein self-organization based on the reversible and energy-dependent interaction of the ATPase MinD and its activating protein MinE with a lipid membrane. By reconstituting MinE mutants impaired in ATPase stimulation, we demonstrate how large-scale Min protein patterns are modulated by MinE activity and concentration. We then provide a perspective on the de novo design of self-organizing protein networks. Tightly integrated reverse and forward engineering approaches will be key to understanding and engineering the intriguing phenomenon of protein pattern formation.This article is part of the theme issue 'Self-organization in cell biology'. © 2018 The Author(s).

  3. Protecting intellectual property in space; Proceedings of the Aerospace Computer Security Conference, McLean, VA, March 20, 1985

    NASA Technical Reports Server (NTRS)

    1985-01-01

    The primary purpose of the Aerospace Computer Security Conference was to bring together people and organizations which have a common interest in protecting intellectual property generated in space. Operational concerns are discussed, taking into account security implications of the space station information system, Space Shuttle security policies and programs, potential uses of probabilistic risk assessment techniques for space station development, key considerations in contingency planning for secure space flight ground control centers, a systematic method for evaluating security requirements compliance, and security engineering of secure ground stations. Subjects related to security technologies are also explored, giving attention to processing requirements of secure C3/I and battle management systems and the development of the Gemini trusted multiple microcomputer base, the Restricted Access Processor system as a security guard designed to protect classified information, and observations on local area network security.

  4. Cryptography and the Internet: lessons and challenges

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McCurley, K.S.

    1996-12-31

    The popularization of the Internet has brought fundamental changes to the world, because it allows a universal method of communication between computers. This carries enormous benefits with it, but also raises many security considerations. Cryptography is a fundamental technology used to provide security of computer networks, and there is currently a widespread engineering effort to incorporate cryptography into various aspects of the Internet. The system-level engineering required to provide security services for the Internet carries some important lessons for researchers whose study is focused on narrowly defined problems. It also offers challenges to the cryptographic research community by raising newmore » questions not adequately addressed by the existing body of knowledge. This paper attempts to summarize some of these lessons and challenges for the cryptographic research community.« less

  5. 46 CFR 128.130 - Vital systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... auxiliaries vital to the vessel's survivability and safety. (10) Any other marine-engineering system... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT... vessel's survivability and safety. For the purpose of this subchapter, the following are vital systems...

  6. 46 CFR 128.130 - Vital systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... auxiliaries vital to the vessel's survivability and safety. (10) Any other marine-engineering system... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT... vessel's survivability and safety. For the purpose of this subchapter, the following are vital systems...

  7. 46 CFR 128.130 - Vital systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... auxiliaries vital to the vessel's survivability and safety. (10) Any other marine-engineering system... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT... vessel's survivability and safety. For the purpose of this subchapter, the following are vital systems...

  8. 46 CFR 128.130 - Vital systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... auxiliaries vital to the vessel's survivability and safety. (10) Any other marine-engineering system... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT... vessel's survivability and safety. For the purpose of this subchapter, the following are vital systems...

  9. 7 CFR 1767.22 - Other income and deductions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... servicing organization for furnishing supervision, management, engineering, and similar services to others... servicing organization for furnishing supervision, management, engineering, and similar services to others... receivable which have not been declared or guaranteed, and interest or dividends upon reacquired securities...

  10. 7 CFR 1767.22 - Other income and deductions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... servicing organization for furnishing supervision, management, engineering, and similar services to others... servicing organization for furnishing supervision, management, engineering, and similar services to others... receivable which have not been declared or guaranteed, and interest or dividends upon reacquired securities...

  11. 7 CFR 1767.22 - Other income and deductions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... servicing organization for furnishing supervision, management, engineering, and similar services to others... servicing organization for furnishing supervision, management, engineering, and similar services to others... receivable which have not been declared or guaranteed, and interest or dividends upon reacquired securities...

  12. A Recommended Framework for the Network-Centric Acquisition Process

    DTIC Science & Technology

    2009-09-01

    ISO /IEC 12207 , Systems and Software Engineering-Software Life-Cycle Processes  ANSI/EIA 632, Processes for Engineering a System. There are...engineering [46]. Some of the process models presented in the DAG are:  ISO /IEC 15288, Systems and Software Engineering-System Life-Cycle Processes...e.g., ISO , IA, Security, etc.). Vetting developers helps ensure that they are using industry best industry practices and maximize the IA compliance

  13. Engineering Emergency: African Americans and Hispanics Lack Pathways to Engineering. Vital Signs: Reports on the Condition of STEM Learning in the U.S.

    ERIC Educational Resources Information Center

    Change the Equation, 2014

    2014-01-01

    A quality education that leads to good jobs offers a reliable pathway to economic security, yet the first step on that pathway remains inaccessible to far too many Americans, especially Americans of color. Nowhere is this inequity more apparent than in engineering. On average, people with engineering bachelor's degrees earn higher salaries than…

  14. 75 FR 76041 - Chrysler Group LLC Formerly Known as Chrysler LLC Kenosha Engine Plant Including On-Site Leased...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    ... Formerly Known as Chrysler LLC Kenosha Engine Plant Including On-Site Leased Workers From Caravan Knight..., K+S Services, Inc., G4S Secure Solutions, Crassociates, Inc., CES, Inc., Evans Distribution Systems..., LLC, formerly known as Chrysler, LLC, Kenosha Engine Plant, Kenosha, Wisconsin (subject firm). The...

  15. 36th International Symposium on Combustion (ISOC2016)

    DTIC Science & Technology

    2016-12-01

    GREENHOUSE GASES / IC ENGINE COMBUSTION I GAS TURBINE COMBUSTION I NOVEL COMBUSTION CONCEPTS, TECHNOLOGIES AND SYSTEMS 15. SUBJECT TERMS Reaction...pollutants and greenhouse gases; IC engine combustion; Gas turbine combustion; Novel combustion concepts, technologies and systems 16. SECURITY...PLENARY LECTURE TRANSFER (15 min) am Turbulent Flames IC Engines Laminar Flames Reaction Kinetics Gas Turbines Soot Solid Fuels/Pollutants

  16. Advanced Functional Nanomaterials for Biological Processes

    DTIC Science & Technology

    2014-01-01

    of this project, we performed research in the area of tissue engineering/bone regeneration and cancer nanotechnology . The primary focus of the tissue...photoacoustic approach. 15. SUBJECT TERMS: Tissue Engineering, Cancer detection, Cancer destruction, Nanoparticles 16. SECURITY CLASSIFICATION OF: 17...Nanocomposite Materials with Drug Delivery Capabilities for Tissue Engineering and Bone Regeneration; and B. Multifunctional Nanoparticles for Cancer Early

  17. Generic comparison of protein inference engines.

    PubMed

    Claassen, Manfred; Reiter, Lukas; Hengartner, Michael O; Buhmann, Joachim M; Aebersold, Ruedi

    2012-04-01

    Protein identifications, instead of peptide-spectrum matches, constitute the biologically relevant result of shotgun proteomics studies. How to appropriately infer and report protein identifications has triggered a still ongoing debate. This debate has so far suffered from the lack of appropriate performance measures that allow us to objectively assess protein inference approaches. This study describes an intuitive, generic and yet formal performance measure and demonstrates how it enables experimentalists to select an optimal protein inference strategy for a given collection of fragment ion spectra. We applied the performance measure to systematically explore the benefit of excluding possibly unreliable protein identifications, such as single-hit wonders. Therefore, we defined a family of protein inference engines by extending a simple inference engine by thousands of pruning variants, each excluding a different specified set of possibly unreliable identifications. We benchmarked these protein inference engines on several data sets representing different proteomes and mass spectrometry platforms. Optimally performing inference engines retained all high confidence spectral evidence, without posterior exclusion of any type of protein identifications. Despite the diversity of studied data sets consistently supporting this rule, other data sets might behave differently. In order to ensure maximal reliable proteome coverage for data sets arising in other studies we advocate abstaining from rigid protein inference rules, such as exclusion of single-hit wonders, and instead consider several protein inference approaches and assess these with respect to the presented performance measure in the specific application context.

  18. Structuring the Chief Information Security Officer Organization

    DTIC Science & Technology

    2015-09-07

    GP9 Objectively Evaluate Adherence CERT-RMM HRM Human Resource Management CERT-RMM ID Identity Management CERT-RMM IMC Incident Management and...Detect, triage, analyze, respond to, and recover from suspicious events and security incidents Security incident management IMC IR IR-1, IR- 2, IR-3...2015-TN-007 | SOFTWARE ENGINEERING INSTITUTE | CARNEGIE MELLON UNIVERSITY 6 Table 2: Source Acronyms3 CERT-RMM NIST 800-53 C2M2 IMC Incident

  19. A model for international border management systems.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Duggan, Ruth Ann

    2008-09-01

    To effectively manage the security or control of its borders, a country must understand its border management activities as a system. Using its systems engineering and security foundations as a Department of Energy National Security Laboratory, Sandia National Laboratories has developed such an approach to modeling and analyzing border management systems. This paper describes the basic model and its elements developed under Laboratory Directed Research and Development project 08-684.

  20. USSOF OPERATIONS IN AFRICA: PROSPECTS FOR FUTURE ENGAGEMENTS IN THE SAHEL

    DTIC Science & Technology

    2017-04-03

    Mechanical Engineering. He also obtained a Master of Arts degree in Terrorism , Organized Crime and Global Security from the Coventry University, United...was a staff officer in the Headquarters Nigerian Air Force, Abuja. iv Abstract As part of the Global War on Terror , AFRICOM was established...to secure the US security interests in Africa. AFRICOM, through the Trans-Saharan Counter Terrorism Partnership uses USSOF to engage Sahelian

  1. A Dynamic Security Framework for Ambient Intelligent Systems: A Smart-Home Based eHealth Application

    NASA Astrophysics Data System (ADS)

    Compagna, Luca; El Khoury, Paul; Massacci, Fabio; Saidane, Ayda

    Providing context-dependent security services is an important challenge for ambient intelligent systems. The complexity and the unbounded nature of such systems make it difficult even for the most experienced and knowledgeable security engineers, to foresee all possible situations and interactions when developing the system. In order to solve this problem context based self- diagnosis and reconfiguration at runtime should be provided.

  2. CrossTalk: The Journal of Defense Software Engineering. Volume 19, Number 5

    DTIC Science & Technology

    2006-05-01

    Coming Events Web Sites Call for Articles Visit CrossTalk at the SSTC BackTalk Transforming: Business , Security ,Warfighting CrossTalk 76 SMXG CO-SPONSOR...theme “Transforming: Business , Security , Warfighting.” Transformation is not just the current buzzword: Many industries and the military have...systems. Transforming: Business , Security ,Warfighting Characteristic edocfosenilnoillim001-01eziS Number of external interfaces 30-300 Number of

  3. A Practical Teaching Course in Directed Protein Evolution Using the Green Fluorescent Protein as a Model

    ERIC Educational Resources Information Center

    Ruller, Roberto; Silva-Rocha, Rafael; Silva, Artur; Schneider, Maria Paula Cruz; Ward, Richard John

    2011-01-01

    Protein engineering is a powerful tool, which correlates protein structure with specific functions, both in applied biotechnology and in basic research. Here, we present a practical teaching course for engineering the green fluorescent protein (GFP) from "Aequorea victoria" by a random mutagenesis strategy using error-prone polymerase…

  4. 46 CFR 193.05-10 - Fixed fire extinguishing systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....05-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH... combustion engine installations; (2) Gas turbine installations; (3) Enclosed spaces containing gasoline engines; (4) Chemical storerooms; (5) Any space containing auxiliaries with an aggregate power of 1,000...

  5. 46 CFR 193.05-10 - Fixed fire extinguishing systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....05-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH... combustion engine installations; (2) Gas turbine installations; (3) Enclosed spaces containing gasoline engines; (4) Chemical storerooms; (5) Any space containing auxiliaries with an aggregate power of 1,000...

  6. 46 CFR 193.05-10 - Fixed fire extinguishing systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....05-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH... combustion engine installations; (2) Gas turbine installations; (3) Enclosed spaces containing gasoline engines; (4) Chemical storerooms; (5) Any space containing auxiliaries with an aggregate power of 1,000...

  7. 46 CFR 112.35-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS... Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

  8. 46 CFR 112.35-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS... Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

  9. 46 CFR 112.35-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS... Generator as the Sole Emergency Power Source § 112.35-1 General. This subpart contains requirements applicable to emergency power installations having a manually controlled storage battery, diesel engine, or...

  10. 46 CFR 182.415 - Carburetors.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... acceptable means of backfire flame control. Installation of backfire flame arresters bearing basic Approval... acceptable means of backfire flame control for gasoline engines: (1) A backfire flame arrester complying with... and firmly secured to withstand vibration, shock, and engine backfire. Such installations do not...

  11. 46 CFR 182.415 - Carburetors.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... acceptable means of backfire flame control. Installation of backfire flame arresters bearing basic Approval... acceptable means of backfire flame control for gasoline engines: (1) A backfire flame arrester complying with... and firmly secured to withstand vibration, shock, and engine backfire. Such installations do not...

  12. 46 CFR 182.415 - Carburetors.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... acceptable means of backfire flame control. Installation of backfire flame arresters bearing basic Approval... acceptable means of backfire flame control for gasoline engines: (1) A backfire flame arrester complying with... and firmly secured to withstand vibration, shock, and engine backfire. Such installations do not...

  13. 46 CFR 182.415 - Carburetors.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... acceptable means of backfire flame control. Installation of backfire flame arresters bearing basic Approval... acceptable means of backfire flame control for gasoline engines: (1) A backfire flame arrester complying with... and firmly secured to withstand vibration, shock, and engine backfire. Such installations do not...

  14. 46 CFR 182.415 - Carburetors.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... acceptable means of backfire flame control. Installation of backfire flame arresters bearing basic Approval... acceptable means of backfire flame control for gasoline engines: (1) A backfire flame arrester complying with... and firmly secured to withstand vibration, shock, and engine backfire. Such installations do not...

  15. Artificially Engineered Protein Polymers.

    PubMed

    Yang, Yun Jung; Holmberg, Angela L; Olsen, Bradley D

    2017-06-07

    Modern polymer science increasingly requires precise control over macromolecular structure and properties for engineering advanced materials and biomedical systems. The application of biological processes to design and synthesize artificial protein polymers offers a means for furthering macromolecular tunability, enabling polymers with dispersities of ∼1.0 and monomer-level sequence control. Taking inspiration from materials evolved in nature, scientists have created modular building blocks with simplified monomer sequences that replicate the function of natural systems. The corresponding protein engineering toolbox has enabled the systematic development of complex functional polymeric materials across areas as diverse as adhesives, responsive polymers, and medical materials. This review discusses the natural proteins that have inspired the development of key building blocks for protein polymer engineering and the function of these elements in material design. The prospects and progress for scalable commercialization of protein polymers are reviewed, discussing both technology needs and opportunities.

  16. Teaching Network Security with IP Darkspace Data

    ERIC Educational Resources Information Center

    Zseby, Tanja; Iglesias Vázquez, Félix; King, Alistair; Claffy, K. C.

    2016-01-01

    This paper presents a network security laboratory project for teaching network traffic anomaly detection methods to electrical engineering students. The project design follows a research-oriented teaching principle, enabling students to make their own discoveries in real network traffic, using data captured from a large IP darkspace monitor…

  17. Enhancing the Security Cooperation Marine Air Ground Task Force to Satisfy the Needs of the Uncertain Global Security Environment

    DTIC Science & Technology

    2009-06-12

    and 2004 National Military Strategy (NMS) continue to germinate . Discussions surrounding the spread of effective democracies and alternate...additional civil-military operation capabilities such as enhanced engineering, additional medical and dental support, and transportation tailored for

  18. Reprogramming cellular functions with engineered membrane proteins.

    PubMed

    Arber, Caroline; Young, Melvin; Barth, Patrick

    2017-10-01

    Taking inspiration from Nature, synthetic biology utilizes and modifies biological components to expand the range of biological functions for engineering new practical devices and therapeutics. While early breakthroughs mainly concerned the design of gene circuits, recent efforts have focused on engineering signaling pathways to reprogram cellular functions. Since signal transduction across cell membranes initiates and controls intracellular signaling, membrane receptors have been targeted by diverse protein engineering approaches despite limited mechanistic understanding of their function. The modular architecture of several receptor families has enabled the empirical construction of chimeric receptors combining domains from distinct native receptors which have found successful immunotherapeutic applications. Meanwhile, progress in membrane protein structure determination, computational modeling and rational design promise to foster the engineering of a broader range of membrane receptor functions. Marrying empirical and rational membrane protein engineering approaches should enable the reprogramming of cells with widely diverse fine-tuned functions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Protein consensus-based surface engineering (ProCoS): a computer-assisted method for directed protein evolution.

    PubMed

    Shivange, Amol V; Hoeffken, Hans Wolfgang; Haefner, Stefan; Schwaneberg, Ulrich

    2016-12-01

    Protein consensus-based surface engineering (ProCoS) is a simple and efficient method for directed protein evolution combining computational analysis and molecular biology tools to engineer protein surfaces. ProCoS is based on the hypothesis that conserved residues originated from a common ancestor and that these residues are crucial for the function of a protein, whereas highly variable regions (situated on the surface of a protein) can be targeted for surface engineering to maximize performance. ProCoS comprises four main steps: ( i ) identification of conserved and highly variable regions; ( ii ) protein sequence design by substituting residues in the highly variable regions, and gene synthesis; ( iii ) in vitro DNA recombination of synthetic genes; and ( iv ) screening for active variants. ProCoS is a simple method for surface mutagenesis in which multiple sequence alignment is used for selection of surface residues based on a structural model. To demonstrate the technique's utility for directed evolution, the surface of a phytase enzyme from Yersinia mollaretii (Ymphytase) was subjected to ProCoS. Screening just 1050 clones from ProCoS engineering-guided mutant libraries yielded an enzyme with 34 amino acid substitutions. The surface-engineered Ymphytase exhibited 3.8-fold higher pH stability (at pH 2.8 for 3 h) and retained 40% of the enzyme's specific activity (400 U/mg) compared with the wild-type Ymphytase. The pH stability might be attributed to a significantly increased (20 percentage points; from 9% to 29%) number of negatively charged amino acids on the surface of the engineered phytase.

  20. Vulnerability survival analysis: a novel approach to vulnerability management

    NASA Astrophysics Data System (ADS)

    Farris, Katheryn A.; Sullivan, John; Cybenko, George

    2017-05-01

    Computer security vulnerabilities span across large, enterprise networks and have to be mitigated by security engineers on a routine basis. Presently, security engineers will assess their "risk posture" through quantifying the number of vulnerabilities with a high Common Vulnerability Severity Score (CVSS). Yet, little to no attention is given to the length of time by which vulnerabilities persist and survive on the network. In this paper, we review a novel approach to quantifying the length of time a vulnerability persists on the network, its time-to-death, and predictors of lower vulnerability survival rates. Our contribution is unique in that we apply the cox proportional hazards regression model to real data from an operational IT environment. This paper provides a mathematical overview of the theory behind survival analysis methods, a description of our vulnerability data, and an interpretation of the results.

  1. Additive Manufacturing and High-Performance Computing: a Disruptive Latent Technology

    NASA Astrophysics Data System (ADS)

    Goodwin, Bruce

    2015-03-01

    This presentation will discuss the relationship between recent advances in Additive Manufacturing (AM) technology, High-Performance Computing (HPC) simulation and design capabilities, and related advances in Uncertainty Quantification (UQ), and then examines their impacts upon national and international security. The presentation surveys how AM accelerates the fabrication process, while HPC combined with UQ provides a fast track for the engineering design cycle. The combination of AM and HPC/UQ almost eliminates the engineering design and prototype iterative cycle, thereby dramatically reducing cost of production and time-to-market. These methods thereby present significant benefits for US national interests, both civilian and military, in an age of austerity. Finally, considering cyber security issues and the advent of the ``cloud,'' these disruptive, currently latent technologies may well enable proliferation and so challenge both nuclear and non-nuclear aspects of international security.

  2. Design and implementation of a high performance network security processor

    NASA Astrophysics Data System (ADS)

    Wang, Haixin; Bai, Guoqiang; Chen, Hongyi

    2010-03-01

    The last few years have seen many significant progresses in the field of application-specific processors. One example is network security processors (NSPs) that perform various cryptographic operations specified by network security protocols and help to offload the computation intensive burdens from network processors (NPs). This article presents a high performance NSP system architecture implementation intended for both internet protocol security (IPSec) and secure socket layer (SSL) protocol acceleration, which are widely employed in virtual private network (VPN) and e-commerce applications. The efficient dual one-way pipelined data transfer skeleton and optimised integration scheme of the heterogenous parallel crypto engine arrays lead to a Gbps rate NSP, which is programmable with domain specific descriptor-based instructions. The descriptor-based control flow fragments large data packets and distributes them to the crypto engine arrays, which fully utilises the parallel computation resources and improves the overall system data throughput. A prototyping platform for this NSP design is implemented with a Xilinx XC3S5000 based FPGA chip set. Results show that the design gives a peak throughput for the IPSec ESP tunnel mode of 2.85 Gbps with over 2100 full SSL handshakes per second at a clock rate of 95 MHz.

  3. Transboundary Water: Improving Methodologies and Developing Integrated Tools to Support Water Security

    NASA Technical Reports Server (NTRS)

    Hakimdavar, Raha; Wood, Danielle; Eylander, John; Peters-Lidard, Christa; Smith, Jane; Doorn, Brad; Green, David; Hummel, Corey; Moore, Thomas C.

    2018-01-01

    River basins for which transboundary coordination and governance is a factor are of concern to US national security, yet there is often a lack of sufficient data-driven information available at the needed time horizons to inform transboundary water decision-making for the intelligence, defense, and foreign policy communities. To address this need, a two-day workshop entitled Transboundary Water: Improving Methodologies and Developing Integrated Tools to Support Global Water Security was held in August 2017 in Maryland. The committee that organized and convened the workshop (the Organizing Committee) included representatives from the National Aeronautics and Space Administration (NASA), the US Army Corps of Engineers Engineer Research and Development Center (ERDC), and the US Air Force. The primary goal of the workshop was to advance knowledge on the current US Government and partners' technical information needs and gaps to support national security interests in relation to transboundary water. The workshop also aimed to identify avenues for greater communication and collaboration among the scientific, intelligence, defense, and foreign policy communities. The discussion around transboundary water was considered in the context of the greater global water challenges facing US national security.

  4. Creation of security engineering programs by the Southwest Surety Institute

    NASA Astrophysics Data System (ADS)

    Romero, Van D.; Rogers, Bradley; Winfree, Tim; Walsh, Dan; Garcia, Mary Lynn

    1998-12-01

    The Southwest Surety Institute includes Arizona State University (ASU), Louisiana State University (LSU), New Mexico Institute of Mining and Technology (NM Tech), New Mexico State University (NMSU), and Sandia National Laboratories (SNL). The universities currently offer a full spectrum of post-secondary programs in security system design and evaluation, including an undergraduate minor, a graduate program, and continuing education programs. The programs are based on the methodology developed at Sandia National Laboratories over the past 25 years to protect critical nuclear assets. The programs combine basic concepts and principles from business, criminal justice, and technology to create an integrated performance-based approach to security system design and analysis. Existing university capabilities in criminal justice (NMSU), explosives testing and technology (NM Tech and LSU), and engineering technology (ASU) are leveraged to provide unique science-based programs that will emphasize the use of performance measures and computer analysis tools to prove the effectiveness of proposed systems in the design phase. Facility managers may then balance increased protection against the cost of implementation and risk mitigation, thereby enabling effective business decisions. Applications expected to benefit from these programs include corrections, law enforcement, counter-terrorism, critical infrastructure protection, financial and medical care fraud, industrial security, and border security.

  5. Joint Operating Environment: The Joint Force in a Contested and Disordered World

    DTIC Science & Technology

    2016-07-14

    spilling over borders, and creating wide-ranging international problems. The future of Science, Technology, and Engineering will see others reaching...10 Science, Technology, and Engineering and the Future Joint Force ..........................................15 Summary... Engineering – may lead to new and challenging conditions that will redefine the security environment of 2035.  Section 2: Contexts of Future Conflict

  6. Modulation of protein stability and aggregation properties by surface charge engineering.

    PubMed

    Raghunathan, Govindan; Sokalingam, Sriram; Soundrarajan, Nagasundarapandian; Madan, Bharat; Munussami, Ganapathiraman; Lee, Sun-Gu

    2013-09-01

    An attempt to alter protein surface charges through traditional protein engineering approaches often affects the native protein structure significantly and induces misfolding. This limitation is a major hindrance in modulating protein properties through surface charge variations. In this study, as a strategy to overcome such a limitation, we attempted to co-introduce stabilizing mutations that can neutralize the destabilizing effect of protein surface charge variation. Two sets of rational mutations were designed; one to increase the number of surface charged amino acids and the other to decrease the number of surface charged amino acids by mutating surface polar uncharged amino acids and charged amino acids, respectively. These two sets of mutations were introduced into Green Fluorescent Protein (GFP) together with or without stabilizing mutations. The co-introduction of stabilizing mutations along with mutations for surface charge modification allowed us to obtain functionally active protein variants (s-GFP(+15-17) and s-GFP(+5-6)). When the protein properties such as fluorescent activity, folding rate and kinetic stability were assessed, we found the possibility that the protein stability can be modulated independently of activity and folding by engineering protein surface charges. The aggregation properties of GFP could also be altered through the surface charge engineering.

  7. 46 CFR 50.01-15 - Scope of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... choice of materials for machinery, boilers, pressure vessels, safety valves, and piping systems upon which safety of life is dependent. (b) Since this subchapter contains the marine engineering details, it... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Basis and...

  8. 46 CFR 50.01-10 - Purpose of regulations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS... in this subchapter is to set forth minimum requirements for marine engineering details for various... thereof or supplemental thereto as well as to implement various international conventions for safety of...

  9. 46 CFR 50.01-15 - Scope of regulations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... choice of materials for machinery, boilers, pressure vessels, safety valves, and piping systems upon which safety of life is dependent. (b) Since this subchapter contains the marine engineering details, it... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Basis and...

  10. 46 CFR 50.01-15 - Scope of regulations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... choice of materials for machinery, boilers, pressure vessels, safety valves, and piping systems upon which safety of life is dependent. (b) Since this subchapter contains the marine engineering details, it... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Basis and...

  11. 46 CFR 50.01-15 - Scope of regulations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... choice of materials for machinery, boilers, pressure vessels, safety valves, and piping systems upon which safety of life is dependent. (b) Since this subchapter contains the marine engineering details, it... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Basis and...

  12. 46 CFR 50.01-10 - Purpose of regulations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS... in this subchapter is to set forth minimum requirements for marine engineering details for various... thereof or supplemental thereto as well as to implement various international conventions for safety of...

  13. 46 CFR 50.01-15 - Scope of regulations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... choice of materials for machinery, boilers, pressure vessels, safety valves, and piping systems upon which safety of life is dependent. (b) Since this subchapter contains the marine engineering details, it... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Basis and...

  14. 46 CFR 50.01-10 - Purpose of regulations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS... in this subchapter is to set forth minimum requirements for marine engineering details for various... thereof or supplemental thereto as well as to implement various international conventions for safety of...

  15. 46 CFR 50.01-10 - Purpose of regulations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS... in this subchapter is to set forth minimum requirements for marine engineering details for various... thereof or supplemental thereto as well as to implement various international conventions for safety of...

  16. 46 CFR 50.01-10 - Purpose of regulations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS... in this subchapter is to set forth minimum requirements for marine engineering details for various... thereof or supplemental thereto as well as to implement various international conventions for safety of...

  17. 46 CFR 193.05-10 - Fixed fire extinguishing systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....05-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH... provided for internal combustion engine installations in accordance with the following: (1) Enclosed spaces containing gasoline engines shall have fixed carbon dioxide systems. (2) If a fire extinguishing system is...

  18. 46 CFR 193.05-10 - Fixed fire extinguishing systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....05-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH... provided for internal combustion engine installations in accordance with the following: (1) Enclosed spaces containing gasoline engines shall have fixed carbon dioxide systems. (2) If a fire extinguishing system is...

  19. 46 CFR 112.25-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

  20. 46 CFR 112.25-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

  1. 46 CFR 112.25-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

  2. 46 CFR 112.25-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

  3. 46 CFR 112.25-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-1 General. This subpart contains requirements applicable to...

  4. 7 CFR 1767.17 - Operating expense instructions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Accounts § 1767.17 Operating expense instructions. (a) Supervision and engineering. The supervision and engineering includible in the operating expense accounts shall consist of the salary, employee pensions and benefits, social security and other payroll taxes, injuries and damages, and other expenses of...

  5. 14 CFR 49.41 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Specifically Identified Aircraft Engines and Propellers § 49.41 Applicability. This subpart applies to the recording of the following kinds of conveyances... aircraft engine of 550 or more rated takeoff horsepower, or the equivalent of that horsepower, or a...

  6. 14 CFR 49.41 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Specifically Identified Aircraft Engines and Propellers § 49.41 Applicability. This subpart applies to the recording of the following kinds of conveyances... aircraft engine of 550 or more rated takeoff horsepower, or the equivalent of that horsepower, or a...

  7. 14 CFR 49.41 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Specifically Identified Aircraft Engines and Propellers § 49.41 Applicability. This subpart applies to the recording of the following kinds of conveyances... aircraft engine of 550 or more rated takeoff horsepower, or the equivalent of that horsepower, or a...

  8. 14 CFR 49.41 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Specifically Identified Aircraft Engines and Propellers § 49.41 Applicability. This subpart applies to the recording of the following kinds of conveyances... aircraft engine of 550 or more rated takeoff horsepower, or the equivalent of that horsepower, or a...

  9. 14 CFR 49.41 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AIRCRAFT TITLES AND SECURITY DOCUMENTS Encumbrances Against Specifically Identified Aircraft Engines and Propellers § 49.41 Applicability. This subpart applies to the recording of the following kinds of conveyances... aircraft engine of 550 or more rated takeoff horsepower, or the equivalent of that horsepower, or a...

  10. 46 CFR 50.05-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Application § 50.05-1 General. (a) The regulations in this subchapter shall apply to the marine engineering details of... regulations. (e) Industrial systems and components on mobile offshore drilling units must meet subpart 58.60...

  11. 46 CFR 50.05-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Application § 50.05-1 General. (a) The regulations in this subchapter shall apply to the marine engineering details of... regulations. (e) Industrial systems and components on mobile offshore drilling units must meet subpart 58.60...

  12. 46 CFR 50.05-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Application § 50.05-1 General. (a) The regulations in this subchapter shall apply to the marine engineering details of... regulations. (e) Industrial systems and components on mobile offshore drilling units must meet subpart 58.60...

  13. 46 CFR 50.05-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Application § 50.05-1 General. (a) The regulations in this subchapter shall apply to the marine engineering details of... regulations. (e) Industrial systems and components on mobile offshore drilling units must meet subpart 58.60...

  14. 46 CFR 50.05-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING GENERAL PROVISIONS Application § 50.05-1 General. (a) The regulations in this subchapter shall apply to the marine engineering details of... regulations. (e) Industrial systems and components on mobile offshore drilling units must meet subpart 58.60...

  15. 46 CFR 169.605 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false General. 169.605 Section 169.605 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Machinery and Electrical... engine cooling water temperature, exhaust cooling water temperature and engine lubricating oil pressure...

  16. 46 CFR 196.15-10 - Sanitation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Sanitation. 196.15-10 Section 196.15-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS OPERATIONS Test... chief engineer shall be responsible only for the sanitary condition of the engineering department. ...

  17. 46 CFR 196.15-10 - Sanitation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Sanitation. 196.15-10 Section 196.15-10 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS OPERATIONS Test... chief engineer shall be responsible only for the sanitary condition of the engineering department. ...

  18. 40 CFR 90.612 - Exemptions and exclusions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... be imported by any person for purposes related to a business or the public interest. Such purposes do... or exclusions are permitted by this paragraph: (1) National security exemption. An engine may be imported under the national security exemption found at § 90.908. (2) Hardship exemption. The Administrator...

  19. 40 CFR 90.612 - Exemptions and exclusions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... be imported by any person for purposes related to a business or the public interest. Such purposes do... or exclusions are permitted by this paragraph: (1) National security exemption. An engine may be imported under the national security exemption found at § 90.908. (2) Hardship exemption. The Administrator...

  20. Energy and technology review

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Quirk, W.J.; Canada, J.; de Vore, L.

    1994-04-01

    This issue highlights the Lawrence Livermore National Laboratory`s 1993 accomplishments in our mission areas and core programs: economic competitiveness, national security, energy, the environment, lasers, biology and biotechnology, engineering, physics, chemistry, materials science, computers and computing, and science and math education. Secondary topics include: nonproliferation, arms control, international security, environmental remediation, and waste management.

  1. 75 FR 31807 - Federal Property Suitable as Facilities To Assist the Homeless; Republication

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-04

    .... Albert Johnson, Department of the Navy, Asset Management Division, Naval Facilities Engineering Command..., and 75A Reasons: Secured Area Bldgs. 3550, 3551 Naval Base San Diego CA Landholding Agency: Navy... Reasons: Secured Area Maine Bldgs. B496 and 497 Bangor Internatl Airport Bangor ME 04401 Landholding...

  2. 78 FR 76117 - Meeting of the National Commission on the Structure of the Air Force

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-16

    ... missions such as Homeland Defense, Homeland Security, and Defense Support to Civil Agencies. This will... Support, Training and Education, and other specific mission sets such as security forces, civil engineering and science and technology. --Projections and assumptions about future resource levels that will...

  3. 78 FR 76119 - Meeting of the National Commission on the Structure of the Air Force

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-16

    ... missions such as Homeland Defense, Homeland Security, and Defense Support to Civil Agencies. This will... Support, Training and Education, and other specific mission sets such as security forces, civil engineering and science and technology. --Projections and assumptions about future resource levels that will...

  4. 76 FR 6637 - Assumption Buster Workshop: Defense-in-Depth Is a Smart Investment for Cyber Security

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ... Technology Research and Development (NITRD) Program. ACTION: Call for participation. FOR FURTHER INFORMATION... regulations and restrictions. SUMMARY: The NCO, on behalf of the Special Cyber Operations Research and Engineering (SCORE) Committee, an interagency working group that coordinates cyber security research...

  5. Engine out of the Chassis: Cell-Free Protein Synthesis and its Uses

    PubMed Central

    Rosenblum, Gabriel; Cooperman, Barry S.

    2013-01-01

    The translation machinery is the engine of life. Extracting the cytoplasmic milieu from a cell affords a lysate capable of producing proteins in concentrations reaching tens of micromolar. Such lysates, derivable from a variety of cells, allow the facile addition and subtraction of components that are directly or indirectly related to the translation machinery and/or the over-expressed protein. The flexible nature of such cell-free expression systems, when coupled with high throughput monitoring, can be especially suitable for protein engineering studies, allowing one to bypass multiple steps typically required using conventional in vivo protein expression. PMID:24161673

  6. Protein engineering in designing tailored enzymes and microorganisms for biofuels production

    PubMed Central

    Wen, Fei; Nair, Nikhil U; Zhao, Huimin

    2009-01-01

    Summary Lignocellulosic biofuels represent a sustainable, renewable, and the only foreseeable alternative energy source to transportation fossil fuels. However, the recalcitrant nature of lignocellulose poses technical hurdles to an economically viable biorefinery. Low enzymatic hydrolysis efficiency and low productivity, yield, and titer of biofuels are among the top cost contributors. Protein engineering has been used to improve the performances of lignocellulose-degrading enzymes, as well as proteins involved in biofuel synthesis pathways. Unlike its great success seen in other industrial applications, protein engineering has achieved only modest results in improving the lignocellulose-to-biofuels efficiency. This review will discuss the unique challenges that protein engineering faces in the process of converting lignocellulose to biofuels and how they are addressed by recent advances in this field. PMID:19660930

  7. Perimeter security for Minnesota correctional facilities

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Crist, D.; Spencer, D.D.

    1996-12-31

    For the past few years, the Minnesota Department of Corrections, assisted by Sandia National Laboratories, has developed a set of standards for perimeter security at medium, close, and maximum custody correctional facilities in the state. During this process, the threat to perimeter security was examined and concepts about correctional perimeter security were developed. This presentation and paper will review the outcomes of this effort, some of the lessons learned, and the concepts developed during this process and in the course of working with architects, engineers and construction firms as the state upgraded perimeter security at some facilities and planned newmore » construction at other facilities.« less

  8. Software To Secure Distributed Propulsion Simulations

    NASA Technical Reports Server (NTRS)

    Blaser, Tammy M.

    2003-01-01

    Distributed-object computing systems are presented with many security threats, including network eavesdropping, message tampering, and communications middleware masquerading. NASA Glenn Research Center, and its industry partners, has taken an active role in mitigating the security threats associated with developing and operating their proprietary aerospace propulsion simulations. In particular, they are developing a collaborative Common Object Request Broker Architecture (CORBA) Security (CORBASec) test bed to secure their distributed aerospace propulsion simulations. Glenn has been working with its aerospace propulsion industry partners to deploy the Numerical Propulsion System Simulation (NPSS) object-based technology. NPSS is a program focused on reducing the cost and time in developing aerospace propulsion engines

  9. Engineering Proteins for Thermostability with iRDP Web Server

    PubMed Central

    Ghanate, Avinash; Ramasamy, Sureshkumar; Suresh, C. G.

    2015-01-01

    Engineering protein molecules with desired structure and biological functions has been an elusive goal. Development of industrially viable proteins with improved properties such as stability, catalytic activity and altered specificity by modifying the structure of an existing protein has widely been targeted through rational protein engineering. Although a range of factors contributing to thermal stability have been identified and widely researched, the in silico implementation of these as strategies directed towards enhancement of protein stability has not yet been explored extensively. A wide range of structural analysis tools is currently available for in silico protein engineering. However these tools concentrate on only a limited number of factors or individual protein structures, resulting in cumbersome and time-consuming analysis. The iRDP web server presented here provides a unified platform comprising of iCAPS, iStability and iMutants modules. Each module addresses different facets of effective rational engineering of proteins aiming towards enhanced stability. While iCAPS aids in selection of target protein based on factors contributing to structural stability, iStability uniquely offers in silico implementation of known thermostabilization strategies in proteins for identification and stability prediction of potential stabilizing mutation sites. iMutants aims to assess mutants based on changes in local interaction network and degree of residue conservation at the mutation sites. Each module was validated using an extensively diverse dataset. The server is freely accessible at http://irdp.ncl.res.in and has no login requirements. PMID:26436543

  10. Engineering Proteins for Thermostability with iRDP Web Server.

    PubMed

    Panigrahi, Priyabrata; Sule, Manas; Ghanate, Avinash; Ramasamy, Sureshkumar; Suresh, C G

    2015-01-01

    Engineering protein molecules with desired structure and biological functions has been an elusive goal. Development of industrially viable proteins with improved properties such as stability, catalytic activity and altered specificity by modifying the structure of an existing protein has widely been targeted through rational protein engineering. Although a range of factors contributing to thermal stability have been identified and widely researched, the in silico implementation of these as strategies directed towards enhancement of protein stability has not yet been explored extensively. A wide range of structural analysis tools is currently available for in silico protein engineering. However these tools concentrate on only a limited number of factors or individual protein structures, resulting in cumbersome and time-consuming analysis. The iRDP web server presented here provides a unified platform comprising of iCAPS, iStability and iMutants modules. Each module addresses different facets of effective rational engineering of proteins aiming towards enhanced stability. While iCAPS aids in selection of target protein based on factors contributing to structural stability, iStability uniquely offers in silico implementation of known thermostabilization strategies in proteins for identification and stability prediction of potential stabilizing mutation sites. iMutants aims to assess mutants based on changes in local interaction network and degree of residue conservation at the mutation sites. Each module was validated using an extensively diverse dataset. The server is freely accessible at http://irdp.ncl.res.in and has no login requirements.

  11. Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Raymond, Amy; Lovell, Scott; Lorimer, Don

    2009-12-01

    With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript. In this report, we compare heterologous protein expression levels from native sequences to that of codon engineered synthetic gene constructs designed by Gene Composer. A test set of proteins including a human kinase (P38{alpha}), viral polymerase (HCV NS5B), and bacterial structural protein (FtsZ) were expressed in both E. colimore » and a cell-free wheat germ translation system. We also compare the protein expression levels in E. coli for a set of 11 different proteins with greatly varied G:C content and codon bias. The results consistently demonstrate that protein yields from codon engineered Gene Composer designs are as good as or better than those achieved from the synonymous native genes. Moreover, structure guided N- and C-terminal deletion constructs designed with the aid of Gene Composer can lead to greater success in gene to structure work as exemplified by the X-ray crystallographic structure determination of FtsZ from Bacillus subtilis. These results validate the Gene Composer algorithms, and suggest that using a combination of synthetic gene and protein construct engineering tools can improve the economics of gene to structure research.« less

  12. Engineering building blocks for self-assembling protein nanoparticles

    PubMed Central

    2010-01-01

    Like natural viruses, manmade protein cages for drug delivery are to be ideally formed by repetitive subunits with self-assembling properties, mimicking viral functions and molecular organization. Naturally formed nanostructures (such as viruses, flagella or simpler protein oligomers) can be engineered to acquire specific traits of interest in biomedicine, for instance through the addition of cell targeting agents for desired biodistribution and specific delivery of associated drugs. However, fully artificial constructs would be highly desirable regarding finest tuning and adaptation to precise therapeutic purposes. Although engineering of protein assembling is still in its infancy, arising principles and promising strategies of protein manipulation point out the rational construction of nanoscale protein cages as a feasible concept, reachable through conventional recombinant DNA technologies and microbial protein production. PMID:21192790

  13. Protein Design for Nanostructural Engineering: General Aspects.

    PubMed

    Grove, Tijana Z; Cortajarena, Aitziber L

    2016-01-01

    This chapter aims to introduce the main challenges in the field of protein design for engineering of nanostructures and functional materials. First, we introduce proteins and illustrate the key characteristics that open many possibilities for the use of proteins in nanotechnology. Then, we describe the current state of the art of nanopatterning techniques and the actual needs of the emerging field of nanotechnology to develop new tools in order to achieve precise control and manipulation of elements at the nanoscale. In this sense, the increasing knowledge of protein science and advances in protein design allow to tackle current challenges such as the design of nanodevices, nanopatterned surfaces, and nanomachines. This book highlights the recent progresses of protein nanotechnology over the last decade and emphasizes the power of protein engineering through illustrative examples of protein based-assemblies and their potential applications.

  14. Surface Sites for Engineering Allosteric Control in Proteins

    PubMed Central

    Lee, Jeeyeon; Natarajan, Madhusudan; Nashine, Vishal C.; Socolich, Michael; Vo, Tina; Russ, William P.; Benkovic, Stephen J.; Ranganathan, Rama

    2010-01-01

    Statistical analyses of protein families reveal networks of coevolving amino acids that functionally link distantly positioned functional surfaces. Such linkages suggest a concept for engineering allosteric control into proteins: The intramolecular networks of two proteins could be joined across their surface sites such that the activity of one protein might control the activity of the other. We tested this idea by creating PAS-DHFR, a designed chimeric protein that connects a light-sensing signaling domain from a plant member of the Per/Arnt/Sim (PAS) family of proteins with Escherichia coli dihydrofolate reductase (DHFR). With no optimization, PAS-DHFR exhibited light-dependent catalytic activity that depended on the site of connection and on known signaling mechanisms in both proteins. PAS-DHFR serves as a proof of concept for engineering regulatory activities into proteins through interface design at conserved allosteric sites. PMID:18927392

  15. Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings

    PubMed Central

    Raphel, Jordan; Parisi-Amon, Andreina; Heilshorn, Sarah

    2012-01-01

    Photocrosslinkable, protein-engineered biomaterials combine a rapid, controllable, cytocompatible crosslinking method with a modular design strategy to create a new family of bioactive materials. These materials have a wide range of biomedical applications, including the development of bioactive implant coatings, drug delivery vehicles, and tissue engineering scaffolds. We present the successful functionalization of a bioactive elastin-like protein with photoreactive diazirine moieties. Scalable synthesis is achieved using a standard recombinant protein expression host followed by site-specific modification of lysine residues with a heterobifunctional N-hydroxysuccinimide ester-diazirine crosslinker. The resulting biomaterial is demonstrated to be processable by spin coating, drop casting, soft lithographic patterning, and mold casting to fabricate a variety of two- and three-dimensional photocrosslinked biomaterials with length scales spanning the nanometer to millimeter range. Protein thin films proved to be highly stable over a three-week period. Cell-adhesive functional domains incorporated into the engineered protein materials were shown to remain active post-photo-processing. Human adipose-derived stem cells achieved faster rates of cell adhesion and larger spread areas on thin films of the engineered protein compared to control substrates. The ease and scalability of material production, processing versatility, and modular bioactive functionality make this recombinantly engineered protein an ideal candidate for the development of novel biomaterial coatings, films, and scaffolds. PMID:23015764

  16. Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings.

    PubMed

    Raphel, Jordan; Parisi-Amon, Andreina; Heilshorn, Sarah

    2012-10-07

    Photocrosslinkable, protein-engineered biomaterials combine a rapid, controllable, cytocompatible crosslinking method with a modular design strategy to create a new family of bioactive materials. These materials have a wide range of biomedical applications, including the development of bioactive implant coatings, drug delivery vehicles, and tissue engineering scaffolds. We present the successful functionalization of a bioactive elastin-like protein with photoreactive diazirine moieties. Scalable synthesis is achieved using a standard recombinant protein expression host followed by site-specific modification of lysine residues with a heterobifunctional N-hydroxysuccinimide ester-diazirine crosslinker. The resulting biomaterial is demonstrated to be processable by spin coating, drop casting, soft lithographic patterning, and mold casting to fabricate a variety of two- and three-dimensional photocrosslinked biomaterials with length scales spanning the nanometer to millimeter range. Protein thin films proved to be highly stable over a three-week period. Cell-adhesive functional domains incorporated into the engineered protein materials were shown to remain active post-photo-processing. Human adipose-derived stem cells achieved faster rates of cell adhesion and larger spread areas on thin films of the engineered protein compared to control substrates. The ease and scalability of material production, processing versatility, and modular bioactive functionality make this recombinantly engineered protein an ideal candidate for the development of novel biomaterial coatings, films, and scaffolds.

  17. Apatite nano-crystalline surface modification of poly(lactide-co-glycolide) sintered microsphere scaffolds for bone tissue engineering: implications for protein adsorption.

    PubMed

    Jabbarzadeh, Ehsan; Nair, Lakshmi S; Khan, Yusuf M; Deng, Meng; Laurencin, Cato T

    2007-01-01

    A number of bone tissue engineering approaches are aimed at (i) increasing the osteconductivity and osteoinductivity of matrices, and (ii) incorporating bioactive molecules within the scaffolds. In this study we examined the growth of a nano-crystalline mineral layer on poly(lactide-co-glycolide) (PLAGA) sintered microsphere scaffolds for tissue engineering. In addition, the influence of the mineral precipitate layer on protein adsorption on the scaffolds was studied. Scaffolds were mineralized by incubation in simulated body fluid (SBF). Scanning electron microscopy (SEM) analysis revealed that mineralized scaffolds possess a rough surface with a plate-like nanostructure covering the surface of microspheres. The results of protein adsorption and release studies showed that while the protein release pattern was similar for PLAGA and mineralized PLAGA scaffolds, precipitation of the mineral layer on PLAGA led to enhanced protein adsorption and slower protein release. Mineralization of tissue-engineered surfaces provides a method for both imparting bioactivity and controlling levels of protein adsorption and release.

  18. Integrating the protein and metabolic engineering toolkits for next-generation chemical biosynthesis.

    PubMed

    Pirie, Christopher M; De Mey, Marjan; Jones Prather, Kristala L; Ajikumar, Parayil Kumaran

    2013-04-19

    Through microbial engineering, biosynthesis has the potential to produce thousands of chemicals used in everyday life. Metabolic engineering and synthetic biology are fields driven by the manipulation of genes, genetic regulatory systems, and enzymatic pathways for developing highly productive microbial strains. Fundamentally, it is the biochemical characteristics of the enzymes themselves that dictate flux through a biosynthetic pathway toward the product of interest. As metabolic engineers target sophisticated secondary metabolites, there has been little recognition of the reduced catalytic activity and increased substrate/product promiscuity of the corresponding enzymes compared to those of central metabolism. Thus, fine-tuning these enzymatic characteristics through protein engineering is paramount for developing high-productivity microbial strains for secondary metabolites. Here, we describe the importance of protein engineering for advancing metabolic engineering of secondary metabolism pathways. This pathway integrated enzyme optimization can enhance the collective toolkit of microbial engineering to shape the future of chemical manufacturing.

  19. Energy Optimization Audit at Humphreys Engineer Center

    DTIC Science & Technology

    2008-09-01

    EPDM (ethylene propylene diene M- class [ rubber ]). Doors There are three pairs of doors to the interior terrace (Figure 6) and exit with a high...System EISA Energy Independence and Security Act EPAct Energy Policy Act EPDM EPDM (ethylene propylene diene M-class [ rubber ]) ERDC Engineer

  20. 77 FR 44678 - Kimberly-Clark Worldwide, Inc., a Subsidiary of Kimberly-Clark Corporation, Everett Mill...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-30

    ..., UNISEVE Corporation, Jacobs Engineering, STAFFLOGIX Corporation, and Swift Trucking,Everett, WA; Amended... Security Services, Healthforce, UNISEVE Corporation, and Jacobs Engineering, Everett, Washington. The... STAFFLOGIX Corporation. The subject firm produces tissue products and wood pulp. Following the allegation...

  1. 46 CFR 77.03-1 - Installation and details.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS VESSEL CONTROL AND MISCELLANEOUS SYSTEMS AND EQUIPMENT Marine Engineering Systems § 77.03-1 Installation and details. (a) The installation of all systems of a marine engineering nature, together with the details of design, construction...

  2. 46 CFR 64.2 - Incorporation by reference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Incorporation by reference. 64.2 Section 64.2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND... sections affected, are: American Society of Mechanical Engineers (ASME) International Three Park Avenue...

  3. 46 CFR 128.320 - Exhaust systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Exhaust systems. 128.320 Section 128.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT AND SYSTEMS Main and Auxiliary Machinery § 128.320 Exhaust systems. No diesel-engine exhaust system...

  4. 46 CFR 128.320 - Exhaust systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Exhaust systems. 128.320 Section 128.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT AND SYSTEMS Main and Auxiliary Machinery § 128.320 Exhaust systems. No diesel-engine exhaust system...

  5. 46 CFR 128.320 - Exhaust systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Exhaust systems. 128.320 Section 128.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT AND SYSTEMS Main and Auxiliary Machinery § 128.320 Exhaust systems. No diesel-engine exhaust system...

  6. 46 CFR 128.320 - Exhaust systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Exhaust systems. 128.320 Section 128.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT AND SYSTEMS Main and Auxiliary Machinery § 128.320 Exhaust systems. No diesel-engine exhaust system...

  7. 46 CFR 128.320 - Exhaust systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Exhaust systems. 128.320 Section 128.320 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS MARINE ENGINEERING: EQUIPMENT AND SYSTEMS Main and Auxiliary Machinery § 128.320 Exhaust systems. No diesel-engine exhaust system...

  8. Secured independent tools in peritoneoscopy.

    PubMed

    Tsin, Daniel A; Davila, Fausto; Dominguez, Guillermo; Manolas, Panagiotis

    2010-01-01

    Secured independent tools are being introduced to aid in peritoneoscopy. We present a simple technique for anchoring instruments, powered lights, and micro machines through the abdominal wall. We used a laparoscopic trainer, micro alligator clips with one or two 2-0 nylon tails and cables for engines and lights. The above instruments were introduced via a 12-mm or 15-mm port. Clips were placed for traction, retraction and exposure, lights for illumination, and motors for potential work. A laparoscopy port closure or suture passer was introduced percutaneously to grab and extract the tails or cables outside of the simulated abdominal cavity. The engines and lights were powered by a direct electric current (DC) plugged into exteriorized cables. We used 2 to 3 clips for each, and engines performed well. This basic simulation adds independent instruments, lights, and engines. We replaced cannulas with threads or cables in an attempt to limit the number of ports. This technique further opens the door for innovations in wired machines in laparoscopy, single-port laparoscopy, or natural orifice surgery.

  9. Computationally mapping sequence space to understand evolutionary protein engineering.

    PubMed

    Armstrong, Kathryn A; Tidor, Bruce

    2008-01-01

    Evolutionary protein engineering has been dramatically successful, producing a wide variety of new proteins with altered stability, binding affinity, and enzymatic activity. However, the success of such procedures is often unreliable, and the impact of the choice of protein, engineering goal, and evolutionary procedure is not well understood. We have created a framework for understanding aspects of the protein engineering process by computationally mapping regions of feasible sequence space for three small proteins using structure-based design protocols. We then tested the ability of different evolutionary search strategies to explore these sequence spaces. The results point to a non-intuitive relationship between the error-prone PCR mutation rate and the number of rounds of replication. The evolutionary relationships among feasible sequences reveal hub-like sequences that serve as particularly fruitful starting sequences for evolutionary search. Moreover, genetic recombination procedures were examined, and tradeoffs relating sequence diversity and search efficiency were identified. This framework allows us to consider the impact of protein structure on the allowed sequence space and therefore on the challenges that each protein presents to error-prone PCR and genetic recombination procedures.

  10. Neurosecurity: security and privacy for neural devices.

    PubMed

    Denning, Tamara; Matsuoka, Yoky; Kohno, Tadayoshi

    2009-07-01

    An increasing number of neural implantable devices will become available in the near future due to advances in neural engineering. This discipline holds the potential to improve many patients' lives dramatically by offering improved-and in some cases entirely new-forms of rehabilitation for conditions ranging from missing limbs to degenerative cognitive diseases. The use of standard engineering practices, medical trials, and neuroethical evaluations during the design process can create systems that are safe and that follow ethical guidelines; unfortunately, none of these disciplines currently ensure that neural devices are robust against adversarial entities trying to exploit these devices to alter, block, or eavesdrop on neural signals. The authors define "neurosecurity"-a version of computer science security principles and methods applied to neural engineering-and discuss why neurosecurity should be a critical consideration in the design of future neural devices.

  11. Protocol vulnerability detection based on network traffic analysis and binary reverse engineering.

    PubMed

    Wen, Shameng; Meng, Qingkun; Feng, Chao; Tang, Chaojing

    2017-01-01

    Network protocol vulnerability detection plays an important role in many domains, including protocol security analysis, application security, and network intrusion detection. In this study, by analyzing the general fuzzing method of network protocols, we propose a novel approach that combines network traffic analysis with the binary reverse engineering method. For network traffic analysis, the block-based protocol description language is introduced to construct test scripts, while the binary reverse engineering method employs the genetic algorithm with a fitness function designed to focus on code coverage. This combination leads to a substantial improvement in fuzz testing for network protocols. We build a prototype system and use it to test several real-world network protocol implementations. The experimental results show that the proposed approach detects vulnerabilities more efficiently and effectively than general fuzzing methods such as SPIKE.

  12. Legacy System Engineering, VPERC Consortium

    DTIC Science & Technology

    2009-09-01

    REPORT Legacy System Engineering, VPERC Consortium, Final Report, University of Utah for Work Ending Joly 15, 2009. 14. ABSTRACT 16. SECURITY...Engineering, VPERC Consortium, Final Report, University of Utah for Work Ending Joly 15, 2009. Report Title ABSTRACT This paper is one of three...Sons, 1995. [3] Turner MJ, Clough RW, Martin HC, Topp LJ. “Stiffness and deflection analysis of complex structures.” Journal of the Aeronautical

  13. Transient Heat Transfer Properties in a Pulse Detonation Combustor

    DTIC Science & Technology

    2011-03-01

    strategies for future systems. 15. NUMBER OF PAGES 89 14. SUBJECT TERMS Pulse Detonation Engines, PDE , Heat Transfer 16. PRICE CODE 17. SECURITY...GUI Graphical User Interface NPS Naval Postgraduate School PDC Pulse Detonation Combustion PDE Pulse Detonation Engine RPL Rocket...a tactical missile with a Pulse Detonation Engine ( PDE ) and provide greater range for the same amount of fuel as compared to other current

  14. Designing Secure Systems on Reconfigurable Hardware

    DTIC Science & Technology

    2008-07-01

    Jeff White Department of Electrical and Computer Engineering University of California, Santa Barbara Santa Barbara, CA 93106 {nick callegari,valamehr...ece.ucsb.edu, jdwhite08@engineering.ucsb.edu Ryan Kastner Department of Computer Science and Engineering University of California, San Diego La Jolla...Transactions on Design Automation of Electronic Systems (TODAES), Vol. 13, No. 3, July 2008, 1-24 14. ABSTRACT see report 15. SUBJECT TERMS 16

  15. Status, Vision, and Challenges of an Intelligent Distributed Engine Control Architecture (Postprint)

    DTIC Science & Technology

    2007-09-18

    TERMS turbine engine control, engine health management, FADEC , Universal FADEC , Distributed Controls, UF, UF Platform, common FADEC , Generic FADEC ...Modular FADEC , Adaptive Control 16. SECURITY CLASSIFICATION OF: 19a. NAME OF RESPONSIBLE PERSON (Monitor) a. REPORT Unclassified b. ABSTRACT...Eventually the Full Authority Digital Electronic Control ( FADEC ) became the norm. Presently, this control system architecture accounts for 15 to 20% of

  16. The DTIC Review. Volume 5, Number 3. Cybernetics: Enhancing Human Performance

    DTIC Science & Technology

    2001-03-01

    Human Factors Engineering 16. SECURITY CLASSIFICATION OF: 17. LIMITATION 18. NUMBER 19a. NAME OF RESPONSIBLE PERSON OF ABSTRACT OF PAGES Phyllis...2 AD Number: A382305 Corporate Author: Arizona University - Tucson Department of Electrical and Computer Engineering Tucson, AZ...Visualization Aids AD-A382305 Aug 2000 Arizona University - Tucson Department of Electrical and Computer Engineering Tucson, AZ 2 THIS PAGE INTENTIONALLY

  17. Trainer Engineering Report (Final) for MILES. Volume 2. Revision

    DTIC Science & Technology

    1981-04-22

    formerly a separate document, Data Item AOOX. iii/iv 1A , SECURITY CLASSIFICATION OF THIS PAGE (Uhen Deaa Enterecd) ... __ . ....... REPORT DOCUMENTATION...NAVTRAEQUIPCEN, Orlando, FL 32813 3 14. MON’TORING AGENCY NAME & ADDRESS(II dilletent from CoftrollIn OGlue*) IS. SECURITY CLASS. (of thie twoot...OBSOLETE UNCLASSIFIED S/N 0102蓞-6601 I SECURITY CL.ASSIFICATION OF THIS iPAGE fUlses Data EaieteE i • CONTENTS .I * INTRODUCTION 1-1 1.1 1980 MILES 1-1

  18. Focus on Resiliency: A Process-Oriented Approach to Security

    DTIC Science & Technology

    2005-11-01

    by ANSI Std Z39-18 © 2005 Carnegie Mellon University CSI v1.0 2 Agenda About the SEI Characterizing the problem Security, resiliency, and risk A...2005 Carnegie Mellon University CSI v1.0 5 SEI Technical Programs Product Line Systems Dynamic Systems Software Engineering Process Management...University CSI v1.0 7 What is the problem? Is your organization’s security capability sufficient to identify and manage risks that result from failed

  19. Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins.

    PubMed

    Hussain, Hirra; Fisher, David I; Abbott, W Mark; Roth, Robert G; Dickson, Alan J

    2017-10-01

    Certain recombinant proteins are deemed "difficult to express" in mammalian expression systems requiring significant cell and/or process engineering to abrogate expression bottlenecks. With increasing demand for the production of recombinant proteins in mammalian cells, low protein yields can have significant consequences for industrial processes. To investigate the molecular mechanisms that restrict expression of recombinant proteins, naturally secreted model proteins were analyzed from the tissue inhibitors of metalloproteinase (TIMP) protein family. In particular, TIMP-2 and TIMP-3 were subjected to detailed study. TIMP proteins share significant sequence homology (∼50% identity and ∼70% similarity in amino acid sequence). However, they show marked differences in secretion in mammalian expression systems despite this extensive sequence homology. Using these two proteins as models, this study characterized the molecular mechanisms responsible for poor recombinant protein production. Our results reveal that both TIMP-2 and TIMP-3 are detectable at mRNA and protein level within the cell but only TIMP-2 is secreted effectively into the extracellular medium. Analysis of protein localization and the nature of intracellular protein suggest TIMP-3 is severely limited in its post-translational processing. To overcome this challenge, modification of the TIMP-3 sequence to include a furin protease-cleavable pro-sequence resulted in secretion of the modified TIMP-3 protein, however, incomplete processing was observed. Based on the TIMP-3 data, the protein engineering approach was optimized and successfully applied in combination with cell engineering, the overexpression of furin, to another member of the TIMP protein family (the poorly expressed TIMP-4). Use of the described protein engineering strategy resulted in successful secretion of poorly (TIMP-4) and non-secreted (TIMP-3) targets, and presents a novel strategy to enhance the production of "difficult" recombinant targets. Biotechnol. Bioeng. 2017;114: 2348-2359. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  20. Building a National Security Program at a Small School: Identifying Opportunities and Overcoming Challenges

    ERIC Educational Resources Information Center

    Grossman, Michael; Schortgen, Francis

    2016-01-01

    This article offers insights into the overall program development process and--institutional obstacles and constraints notwithstanding--successful introduction of a new national security program at a small liberal arts university at a time of growing institutional prioritization of science, technology, engineering, and mathematics (STEM) programs.…

  1. Academics and National-Security Experts Must Work Together

    ERIC Educational Resources Information Center

    Gansler, Jacques S.; Gast, Alice P.

    2008-01-01

    In the years since the September 11, 2001, terrorist attacks, the federal government's policies that deal with national security have changed significantly. In an effort to prevent the results of science and engineering research from being misused or falling into the wrong hands, government agencies that support studies are placing restrictions on…

  2. Analysis of Alternatives (AoA) of Open Colllaboration and Research Capabilities Collaboratipon in Research and Engineering in Advanced Technology and Education and High-Performance Computing Innovation Center (HPCIC) on the LVOC.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Vrieling, P. Douglas

    2016-01-01

    The Livermore Valley Open Campus (LVOC), a joint initiative of the National Nuclear Security Administration (NNSA), Lawrence Livermore National Laboratory (LLNL), and Sandia National Laboratories (SNL), enhances the national security missions of NNSA by promoting greater collaboration between world-class scientists at the national security laboratories, and their partners in industry and academia. Strengthening the science, technology, and engineering (ST&E) base of our nation is one of the NNSA’s top goals. By conducting coordinated and collaborative programs, LVOC enhances both the NNSA and the broader national science and technology base, and helps to ensure the health of core capabilities at LLNLmore » and SNL. These capabilities must remain strong to enable the laboratories to execute their primary mission for NNSA.« less

  3. Securing Sensitive Flight and Engine Simulation Data Using Smart Card Technology

    NASA Technical Reports Server (NTRS)

    Blaser, Tammy M.

    2003-01-01

    NASA Glenn Research Center has developed a smart card prototype capable of encrypting and decrypting disk files required to run a distributed aerospace propulsion simulation. Triple Data Encryption Standard (3DES) encryption is used to secure the sensitive intellectual property on disk pre, during, and post simulation execution. The prototype operates as a secure system and maintains its authorized state by safely storing and permanently retaining the encryption keys only on the smart card. The prototype is capable of authenticating a single smart card user and includes pre simulation and post simulation tools for analysis and training purposes. The prototype's design is highly generic and can be used to protect any sensitive disk files with growth capability to urn multiple simulations. The NASA computer engineer developed the prototype on an interoperable programming environment to enable porting to other Numerical Propulsion System Simulation (NPSS) capable operating system environments.

  4. 46 CFR 110.20-1 - Equivalents.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Equivalents. 110.20-1 Section 110.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Equivalents... engineering evaluations and tests to demonstrate the equivalence of the substitute. [CGD 94-108, 61 FR 28275...

  5. 46 CFR 110.20-1 - Equivalents.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Equivalents. 110.20-1 Section 110.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Equivalents... engineering evaluations and tests to demonstrate the equivalence of the substitute. [CGD 94-108, 61 FR 28275...

  6. 46 CFR 110.20-1 - Equivalents.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Equivalents. 110.20-1 Section 110.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Equivalents... engineering evaluations and tests to demonstrate the equivalence of the substitute. [CGD 94-108, 61 FR 28275...

  7. The identification of Gender Bias in the U.S. Military

    DTIC Science & Technology

    2018-03-01

    Opinion Form SSA Social Security Administration STEM Science, Technology, Engineering, and Math U.S. United States USN United States Navy xvi THIS...in science, technology, engineering, and math (STEM) related fields, with lifestyle choice being cited by some as the reasoning for this disparity

  8. 46 CFR 113.30-20 - General requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false General requirements. 113.30-20 Section 113.30-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND... for the operation of the vessel, such as the captain's and chief engineer's offices and staterooms...

  9. 46 CFR 113.30-20 - General requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false General requirements. 113.30-20 Section 113.30-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND... for the operation of the vessel, such as the captain's and chief engineer's offices and staterooms...

  10. 46 CFR 113.30-20 - General requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false General requirements. 113.30-20 Section 113.30-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND... for the operation of the vessel, such as the captain's and chief engineer's offices and staterooms...

  11. 46 CFR 113.30-20 - General requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false General requirements. 113.30-20 Section 113.30-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND... for the operation of the vessel, such as the captain's and chief engineer's offices and staterooms...

  12. 46 CFR 113.30-20 - General requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false General requirements. 113.30-20 Section 113.30-20 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND... for the operation of the vessel, such as the captain's and chief engineer's offices and staterooms...

  13. 46 CFR 112.25-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-3 Normal source for...

  14. 46 CFR 112.25-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-3 Normal source for...

  15. 46 CFR 112.35-5 - Manually started emergency systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

  16. 46 CFR 112.25-10 - Transfer of emergency loads.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

  17. 46 CFR 112.25-10 - Transfer of emergency loads.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

  18. 46 CFR 112.35-5 - Manually started emergency systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

  19. 46 CFR 112.25-10 - Transfer of emergency loads.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

  20. 46 CFR 112.25-10 - Transfer of emergency loads.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

  1. 46 CFR 112.35-5 - Manually started emergency systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

  2. 46 CFR 112.35-5 - Manually started emergency systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

  3. 46 CFR 112.50-7 - Compressed air starting.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-7 Compressed... emergency power source. If this compressor supplies other auxiliaries, there must be a non-return valve at...

  4. 46 CFR 112.25-10 - Transfer of emergency loads.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-10 Transfer of emergency loads. (a) When...

  5. 46 CFR 112.25-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-3 Normal source for...

  6. 46 CFR 112.35-5 - Manually started emergency systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....35-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-5 Manually...

  7. 46 CFR 112.25-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-3 Normal source for...

  8. 46 CFR 112.25-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Systems Having an Automatic Starting Diesel Engine or Gas Turbine Driven Emergency Power Source as the Sole Emergency Power Source § 112.25-3 Normal source for...

  9. 46 CFR 64.2 - Incorporation by reference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Incorporation by reference. 64.2 Section 64.2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND... Mechanical Engineers (ASME) International Three Park Avenue, New York, NY 10016-5990. ASME Boiler and...

  10. 46 CFR 64.2 - Incorporation by reference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Incorporation by reference. 64.2 Section 64.2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MARINE PORTABLE TANKS AND... Mechanical Engineers (ASME) International Three Park Avenue, New York, NY 10016-5990. ASME Boiler and...

  11. 46 CFR 110.20-1 - Equivalents.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Equivalents. 110.20-1 Section 110.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Equivalents... engineering evaluations and tests to demonstrate the equivalence of the substitute. [CGD 94-108, 61 FR 28275...

  12. 46 CFR 110.20-1 - Equivalents.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Equivalents. 110.20-1 Section 110.20-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Equivalents... engineering evaluations and tests to demonstrate the equivalence of the substitute. [CGD 94-108, 61 FR 28275...

  13. 78 FR 40511 - RG Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ..., Alliance Engineering, Inc., Washington Group International, Javan & Walter, Inc., Kinetic Technical... Consulting, Crown Security, Eastern Automation, Eds(Hp), Teksystems, URS Corporation, B More Industrial..., Alliance Engineering, Inc., Washington Group International, Javan & Walter, Inc., Kinetic Technical...

  14. 46 CFR 56.95-1 - General (replaces 136).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

  15. 46 CFR 56.95-1 - General (replaces 136).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

  16. 46 CFR 56.95-1 - General (replaces 136).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

  17. 46 CFR 56.95-1 - General (replaces 136).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

  18. 46 CFR 56.95-1 - General (replaces 136).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Inspection § 56.95-1 General (replaces 136). (a) The provisions in this subpart shall apply to... engineering design, and with the material, fabrication, assembly and test requirements of ASME B31.1, as...

  19. 46 CFR 113.35-1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Definitions. 113.35-1 Section 113.35-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING COMMUNICATION AND ALARM SYSTEMS AND EQUIPMENT Engine Order Telegraph Systems § 113.35-1 Definitions. As used in this subpart: (a...

  20. 46 CFR 112.50-3 - Hydraulic starting.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Hydraulic starting. 112.50-3 Section 112.50-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-3 Hydraulic...

  1. 46 CFR 112.50-3 - Hydraulic starting.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Hydraulic starting. 112.50-3 Section 112.50-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-3 Hydraulic...

  2. A malicious pattern detection engine for embedded security systems in the Internet of Things.

    PubMed

    Oh, Doohwan; Kim, Deokho; Ro, Won Woo

    2014-12-16

    With the emergence of the Internet of Things (IoT), a large number of physical objects in daily life have been aggressively connected to the Internet. As the number of objects connected to networks increases, the security systems face a critical challenge due to the global connectivity and accessibility of the IoT. However, it is difficult to adapt traditional security systems to the objects in the IoT, because of their limited computing power and memory size. In light of this, we present a lightweight security system that uses a novel malicious pattern-matching engine. We limit the memory usage of the proposed system in order to make it work on resource-constrained devices. To mitigate performance degradation due to limitations of computation power and memory, we propose two novel techniques, auxiliary shifting and early decision. Through both techniques, we can efficiently reduce the number of matching operations on resource-constrained systems. Experiments and performance analyses show that our proposed system achieves a maximum speedup of 2.14 with an IoT object and provides scalable performance for a large number of patterns.

  3. Engineered elastomeric proteins with dual elasticity can be controlled by a molecular regulator.

    PubMed

    Cao, Yi; Li, Hongbin

    2008-08-01

    Elastomeric proteins are molecular springs that confer excellent mechanical properties to many biological tissues and biomaterials. Depending on the role performed by the tissue or biomaterial, elastomeric proteins can behave as molecular springs or shock absorbers. Here we combine single-molecule atomic force microscopy and protein engineering techniques to create elastomeric proteins that can switch between two distinct types of mechanical behaviour in response to the binding of a molecular regulator. The proteins are mechanically labile by design and behave as entropic springs with an elasticity that is governed by their configurational entropy. However, when a molecular regulator binds to the protein, it switches into a mechanically stable state and can act as a shock absorber. These engineered proteins effectively mimic and combine the two extreme forms of elastic behaviour found in natural elastomeric proteins, and thus represent a new type of smart nanomaterial that will find potential applications in nanomechanics and material sciences.

  4. Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jang, Yeongseon; Choi, Won Tae; Heller, William T.

    Vesicles assembled from folded, globular proteins have potential for functions different from traditional lipid or polymeric vesicles. However, they also present challenges in understanding the assembly process and controlling vesicle properties. From detailed investigation of the assembly behavior of recombinant fusion proteins, this work reports a simple strategy to engineer protein vesicles containing functional, globular domains. This is achieved through tunable self-assembly of recombinant globular fusion proteins containing leucine zippers and elastin-like polypeptides. The fusion proteins form complexes in solution via high affinity binding of the zippers, and transition through dynamic coacervates to stable hollow vesicles upon warming. The thermalmore » driving force, which can be tuned by protein concentration or temperature, controls both vesicle size and whether vesicles are single or bi-layered. Lastly, these results provide critical information to engineer globular protein vesicles via self-assembly with desired size and membrane structure.« less

  5. Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins

    DOE PAGES

    Jang, Yeongseon; Choi, Won Tae; Heller, William T.; ...

    2017-07-27

    Vesicles assembled from folded, globular proteins have potential for functions different from traditional lipid or polymeric vesicles. However, they also present challenges in understanding the assembly process and controlling vesicle properties. From detailed investigation of the assembly behavior of recombinant fusion proteins, this work reports a simple strategy to engineer protein vesicles containing functional, globular domains. This is achieved through tunable self-assembly of recombinant globular fusion proteins containing leucine zippers and elastin-like polypeptides. The fusion proteins form complexes in solution via high affinity binding of the zippers, and transition through dynamic coacervates to stable hollow vesicles upon warming. The thermalmore » driving force, which can be tuned by protein concentration or temperature, controls both vesicle size and whether vesicles are single or bi-layered. Lastly, these results provide critical information to engineer globular protein vesicles via self-assembly with desired size and membrane structure.« less

  6. Dynamics simulations for engineering macromolecular interactions

    NASA Astrophysics Data System (ADS)

    Robinson-Mosher, Avi; Shinar, Tamar; Silver, Pamela A.; Way, Jeffrey

    2013-06-01

    The predictable engineering of well-behaved transcriptional circuits is a central goal of synthetic biology. The artificial attachment of promoters to transcription factor genes usually results in noisy or chaotic behaviors, and such systems are unlikely to be useful in practical applications. Natural transcriptional regulation relies extensively on protein-protein interactions to insure tightly controlled behavior, but such tight control has been elusive in engineered systems. To help engineer protein-protein interactions, we have developed a molecular dynamics simulation framework that simplifies features of proteins moving by constrained Brownian motion, with the goal of performing long simulations. The behavior of a simulated protein system is determined by summation of forces that include a Brownian force, a drag force, excluded volume constraints, relative position constraints, and binding constraints that relate to experimentally determined on-rates and off-rates for chosen protein elements in a system. Proteins are abstracted as spheres. Binding surfaces are defined radially within a protein. Peptide linkers are abstracted as small protein-like spheres with rigid connections. To address whether our framework could generate useful predictions, we simulated the behavior of an engineered fusion protein consisting of two 20 000 Da proteins attached by flexible glycine/serine-type linkers. The two protein elements remained closely associated, as if constrained by a random walk in three dimensions of the peptide linker, as opposed to showing a distribution of distances expected if movement were dominated by Brownian motion of the protein domains only. We also simulated the behavior of fluorescent proteins tethered by a linker of varying length, compared the predicted Förster resonance energy transfer with previous experimental observations, and obtained a good correspondence. Finally, we simulated the binding behavior of a fusion of two ligands that could simultaneously bind to distinct cell-surface receptors, and explored the landscape of linker lengths and stiffnesses that could enhance receptor binding of one ligand when the other ligand has already bound to its receptor, thus, addressing potential mechanisms for improving targeted signal transduction proteins. These specific results have implications for the design of targeted fusion proteins and artificial transcription factors involving fusion of natural domains. More broadly, the simulation framework described here could be extended to include more detailed system features such as non-spherical protein shapes and electrostatics, without requiring detailed, computationally expensive specifications. This framework should be useful in predicting behavior of engineered protein systems including binding and dissociation reactions.

  7. Dynamics simulations for engineering macromolecular interactions.

    PubMed

    Robinson-Mosher, Avi; Shinar, Tamar; Silver, Pamela A; Way, Jeffrey

    2013-06-01

    The predictable engineering of well-behaved transcriptional circuits is a central goal of synthetic biology. The artificial attachment of promoters to transcription factor genes usually results in noisy or chaotic behaviors, and such systems are unlikely to be useful in practical applications. Natural transcriptional regulation relies extensively on protein-protein interactions to insure tightly controlled behavior, but such tight control has been elusive in engineered systems. To help engineer protein-protein interactions, we have developed a molecular dynamics simulation framework that simplifies features of proteins moving by constrained Brownian motion, with the goal of performing long simulations. The behavior of a simulated protein system is determined by summation of forces that include a Brownian force, a drag force, excluded volume constraints, relative position constraints, and binding constraints that relate to experimentally determined on-rates and off-rates for chosen protein elements in a system. Proteins are abstracted as spheres. Binding surfaces are defined radially within a protein. Peptide linkers are abstracted as small protein-like spheres with rigid connections. To address whether our framework could generate useful predictions, we simulated the behavior of an engineered fusion protein consisting of two 20,000 Da proteins attached by flexible glycine/serine-type linkers. The two protein elements remained closely associated, as if constrained by a random walk in three dimensions of the peptide linker, as opposed to showing a distribution of distances expected if movement were dominated by Brownian motion of the protein domains only. We also simulated the behavior of fluorescent proteins tethered by a linker of varying length, compared the predicted Förster resonance energy transfer with previous experimental observations, and obtained a good correspondence. Finally, we simulated the binding behavior of a fusion of two ligands that could simultaneously bind to distinct cell-surface receptors, and explored the landscape of linker lengths and stiffnesses that could enhance receptor binding of one ligand when the other ligand has already bound to its receptor, thus, addressing potential mechanisms for improving targeted signal transduction proteins. These specific results have implications for the design of targeted fusion proteins and artificial transcription factors involving fusion of natural domains. More broadly, the simulation framework described here could be extended to include more detailed system features such as non-spherical protein shapes and electrostatics, without requiring detailed, computationally expensive specifications. This framework should be useful in predicting behavior of engineered protein systems including binding and dissociation reactions.

  8. Coordination and organization of security software process for power information application environment

    NASA Astrophysics Data System (ADS)

    Wang, Qiang

    2017-09-01

    As an important part of software engineering, the software process decides the success or failure of software product. The design and development feature of security software process is discussed, so is the necessity and the present significance of using such process. Coordinating the function software, the process for security software and its testing are deeply discussed. The process includes requirement analysis, design, coding, debug and testing, submission and maintenance. In each process, the paper proposed the subprocesses to support software security. As an example, the paper introduces the above process into the power information platform.

  9. European Science Notes. Volume 40, Number 3.

    DTIC Science & Technology

    1986-03-01

    to protein structures analysis and the UK Institute in Protein Engineering are discussed. Material 9ciences 9cole des Mine de Paris--France’s Premier...ellipsometry and for network analysis tation a.v.); (4) development of a meth- based on a microcomputer. A current R&D od for the rapid production of monoclon...Engineering, Cornell University, Ithaca, New York. Structure Analysis in Protein Engineering, K.M. Ulmer, University of Maryland, Adelphi, Maryland

  10. Molecular Bases of cyclodextrin Adapter Interactions with Engineered Protein Nanopores

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Banerjee, A.; Mikhailova, E; Cheley, S

    2010-01-01

    Engineered protein pores have several potential applications in biotechnology: as sensor elements in stochastic detection and ultrarapid DNA sequencing, as nanoreactors to observe single-molecule chemistry, and in the construction of nano- and micro-devices. One important class of pores contains molecular adapters, which provide internal binding sites for small molecules. Mutants of the {alpha}-hemolysin ({alpha}HL) pore that bind the adapter {beta}-cyclodextrin ({beta}CD) {approx}10{sup 4} times more tightly than the wild type have been obtained. We now use single-channel electrical recording, protein engineering including unnatural amino acid mutagenesis, and high-resolution x-ray crystallography to provide definitive structural information on these engineered protein nanoporesmore » in unparalleled detail.« less

  11. NewProt - a protein engineering portal.

    PubMed

    Schwarte, Andreas; Genz, Maika; Skalden, Lilly; Nobili, Alberto; Vickers, Clare; Melse, Okke; Kuipers, Remko; Joosten, Henk-Jan; Stourac, Jan; Bendl, Jaroslav; Black, Jon; Haase, Peter; Baakman, Coos; Damborsky, Jiri; Bornscheuer, Uwe; Vriend, Gert; Venselaar, Hanka

    2017-06-01

    The NewProt protein engineering portal is a one-stop-shop for in silico protein engineering. It gives access to a large number of servers that compute a wide variety of protein structure characteristics supporting work on the modification of proteins through the introduction of (multiple) point mutations. The results can be inspected through multiple visualizers. The HOPE software is included to indicate mutations with possible undesired side effects. The Hotspot Wizard software is embedded for the design of mutations that modify a proteins' activity, specificity, or stability. The NewProt portal is freely accessible at http://newprot.cmbi.umcn.nl/ and http://newprot.fluidops.net/. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  12. Towards An Engineering Discipline of Computational Security

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mili, Ali; Sheldon, Frederick T; Jilani, Lamia Labed

    2007-01-01

    George Boole ushered the era of modern logic by arguing that logical reasoning does not fall in the realm of philosophy, as it was considered up to his time, but in the realm of mathematics. As such, logical propositions and logical arguments are modeled using algebraic structures. Likewise, we submit that security attributes must be modeled as formal mathematical propositions that are subject to mathematical analysis. In this paper, we approach this problem by attempting to model security attributes in a refinement-like framework that has traditionally been used to represent reliability and safety claims. Keywords: Computable security attributes, survivability, integrity,more » dependability, reliability, safety, security, verification, testing, fault tolerance.« less

  13. Natural and Genetically Engineered Proteins for Tissue Engineering

    PubMed Central

    Gomes, Sílvia; Leonor, Isabel B.; Mano, João F.; Reis, Rui L.

    2011-01-01

    To overcome the limitations of traditionally used autografts, allografts and, to a lesser extent, synthetic materials, there is the need to develop a new generation of scaffolds with adequate mechanical and structural support, control of cell attachment, migration, proliferation and differentiation and with bio-resorbable features. This suite of properties would allow the body to heal itself at the same rate as implant degradation. Genetic engineering offers a route to this level of control of biomaterial systems. The possibility of expressing biological components in nature and to modify or bioengineer them further, offers a path towards multifunctional biomaterial systems. This includes opportunities to generate new protein sequences, new self-assembling peptides or fusions of different bioactive domains or protein motifs. New protein sequences with tunable properties can be generated that can be used as new biomaterials. In this review we address some of the most frequently used proteins for tissue engineering and biomedical applications and describe the techniques most commonly used to functionalize protein-based biomaterials by combining them with bioactive molecules to enhance biological performance. We also highlight the use of genetic engineering, for protein heterologous expression and the synthesis of new protein-based biopolymers, focusing the advantages of these functionalized biopolymers when compared with their counterparts extracted directly from nature and modified by techniques such as physical adsorption or chemical modification. PMID:22058578

  14. 77 FR 74520 - Encore Clean Energy, Inc., Energy & Engine Technology Corp., Equity Media Holdings Corporation...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-14

    ... SECURITIES AND EXCHANGE COMMISSION [File No. 500-1] Encore Clean Energy, Inc., Energy & Engine Technology Corp., Equity Media Holdings Corporation, eTotalSource, Inc., Extensions, Inc., Firepond, Inc., and GNC Energy Corporation; Order Withdrawing Trading Suspension as to Extensions, Inc. December 12...

  15. 46 CFR 13.501 - Original application for “Tankerman-Engineer” endorsement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Original application for âTankerman-Engineerâ endorsement. 13.501 Section 13.501 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE... Original application for “Tankerman-Engineer” endorsement. Each applicant for a “Tankerman-Engineer...

  16. U.S. Energy Prospects: An Engineering Viewpoint.

    ERIC Educational Resources Information Center

    National Academy of Engineering, Washington, DC. Commission on Education.

    With the Arab oil embargo of 1973, the United States became aware of its dependence on foreign fuel to maintain its productive capacity, employment base, political autonomy, strategic security, and living standard. An engineering Task Force on Energy was appointed to provide an informed assessment of the realistic strategies that could be…

  17. Science, engineering and technology—it is a military affair

    NASA Astrophysics Data System (ADS)

    Langley, Chris

    2006-11-01

    Military spending around the world is enormous. One of the key drivers of such spending is research and development, and the major role played here by scientists and engineers is examined in this article, together with broader security threats such as environmental factors that also demand scientific expertise.

  18. 46 CFR 112.35-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

  19. 46 CFR 112.35-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

  20. 46 CFR 112.35-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

  1. 46 CFR 112.35-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

  2. 46 CFR 112.35-3 - Normal source for emergency loads.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....35-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Manually Controlled Emergency Systems Having a Storage Battery or a Diesel Engine or Gas Turbine Driven Generator as the Sole Emergency Power Source § 112.35-3 Normal source...

  3. 46 CFR 63.05-1 - Incorporation by reference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY...: Office of Design and Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther King Jr. Avenue..., March 28, 1986 (“ANSI/AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical...

  4. 46 CFR 63.05-1 - Incorporation by reference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING AUTOMATIC AUXILIARY...: Office of Design and Engineering Systems, U.S. Coast Guard Stop 7509, 2703 Martin Luther King Jr. Avenue..., March 28, 1986 (“ANSI/AGA Z21.22”), 63.25-3; and (2) [Reserved] (c) American Society of Mechanical...

  5. 46 CFR 112.50-5 - Electric starting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Electric starting. 112.50-5 Section 112.50-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-5 Electric starting...

  6. 46 CFR 112.50-5 - Electric starting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Electric starting. 112.50-5 Section 112.50-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-5 Electric starting...

  7. 46 CFR 112.50-5 - Electric starting.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Electric starting. 112.50-5 Section 112.50-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-5 Electric starting...

  8. Fruit metabolite networks in engineered and non-engineered tomato genotypes reveal fluidity in a hormone and agroecosystem specific manner

    USDA-ARS?s Scientific Manuscript database

    Multiple strategies have been explored throughout the world to meet food security. These include molecular breeding, transgenic genotype development, reduced-tillage crop production, modification of the soil environment with cover crops or polyethylene mulches and tunnels, and organic farming. Unde...

  9. 78 FR 19530 - RG Steel Sparrows Point LLC, Formerly Known as Severstal Sparrows Point LLC, a Subsidiary of RG...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-01

    ..., Alliance Engineering, Inc., Washington Group International, Javan & Walter, Inc., Kinetic Technical... Consulting, Crown Security, Eastern Automation, EDS (HP), TekSystems, URS Corporation, B More Industrial... Service Company, Sun Associated Industries, Inc., MPI Consultants LLC, Alliance Engineering, Inc...

  10. 46 CFR 112.50-5 - Electric starting.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Electric starting. 112.50-5 Section 112.50-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-5 Electric starting...

  11. 46 CFR 112.50-5 - Electric starting.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Electric starting. 112.50-5 Section 112.50-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING EMERGENCY LIGHTING AND POWER SYSTEMS Emergency Diesel and Gas Turbine Engine Driven Generator Sets § 112.50-5 Electric starting...

  12. STEM Leader from the Roeper School: An Interview with Nuclear Engineer Clair J. Sullivan

    ERIC Educational Resources Information Center

    Ambrose, Don

    2016-01-01

    Clair J. Sullivan is an assistant professor in the Department of Nuclear, Plasma and Radiological Engineering at the University of Illinois at Urbana-Champaign (UIUC). Her research interests include radiation detection and measurements; gamma-ray spectroscopy; automated isotope identification algorithms; nuclear forensics; nuclear security;…

  13. Engineering Curriculum as Affected by Corporate Giving.

    ERIC Educational Resources Information Center

    Pettigrew, Chenits, Jr.; Hiatt, Diana Buell

    This study investigated the effects of corporate financial support on the University of California Los Angeles (UCLA) School of Engineering and Applied Sciences (SEAS) curriculum. The study evolved out of growing concerns that many university educators have related to the mushrooming of university efforts to secure corporate financial support…

  14. Design of Light-Controlled Protein Conformations and Functions.

    PubMed

    Ritterson, Ryan S; Hoersch, Daniel; Barlow, Kyle A; Kortemme, Tanja

    2016-01-01

    In recent years, interest in controlling protein function with light has increased. Light offers a number of unique advantages over other methods, including spatial and temporal control and high selectivity. Here, we describe a general protocol for engineering a protein to be controllable with light via reaction with an exogenously introduced photoisomerizable small molecule and illustrate our protocol with two examples from the literature: the engineering of the calcium affinity of the cell-cell adhesion protein cadherin, which is an example of a protein that switches from a native to a disrupted state (Ritterson et al. J Am Chem Soc (2013) 135:12516-12519), and the engineering of the opening and closing of the chaperonin Mm-cpn, an example of a switch between two functional states (Hoersch et al.: Nat Nanotechn (2013) 8:928-932). This protocol guides the user from considering which proteins may be most amenable to this type of engineering, to considerations of how and where to make the desired changes, to the assays required to test for functionality.

  15. Environmental Impact Statement for Proposed Closure of Los Angeles Air Force Base, California and Relocation of Space Systems Division

    DTIC Science & Technology

    1990-05-01

    FFRDC); and the Systems Engineering Technical Assistance (SETA) contractor to selected Air Force bases including: Vandenberg AFB California; March AFB...05/21/90 comptroller, acquisition civil engineering , legal, security, communications-computer systems, product assurance, and safety, among others...housing units were constructed in 1983. The Fort MacArthur Housing Area also includes administrative offices, several warehouses, and civil engineering

  16. Protein mechanics: from single molecules to functional biomaterials.

    PubMed

    Li, Hongbin; Cao, Yi

    2010-10-19

    Elastomeric proteins act as the essential functional units in a wide variety of biomechanical machinery and serve as the basic building blocks for biological materials that exhibit superb mechanical properties. These proteins provide the desired elasticity, mechanical strength, resilience, and toughness within these materials. Understanding the mechanical properties of elastomeric protein-based biomaterials is a multiscale problem spanning from the atomistic/molecular level to the macroscopic level. Uncovering the design principles of individual elastomeric building blocks is critical both for the scientific understanding of multiscale mechanics of biomaterials and for the rational engineering of novel biomaterials with desirable mechanical properties. The development of single-molecule force spectroscopy techniques has provided methods for characterizing mechanical properties of elastomeric proteins one molecule at a time. Single-molecule atomic force microscopy (AFM) is uniquely suited to this purpose. Molecular dynamic simulations, protein engineering techniques, and single-molecule AFM study have collectively revealed tremendous insights into the molecular design of single elastomeric proteins, which can guide the design and engineering of elastomeric proteins with tailored mechanical properties. Researchers are focusing experimental efforts toward engineering artificial elastomeric proteins with mechanical properties that mimic or even surpass those of natural elastomeric proteins. In this Account, we summarize our recent experimental efforts to engineer novel artificial elastomeric proteins and develop general and rational methodologies to tune the nanomechanical properties of elastomeric proteins at the single-molecule level. We focus on general design principles used for enhancing the mechanical stability of proteins. These principles include the development of metal-chelation-based general methodology, strategies to control the unfolding hierarchy of multidomain elastomeric proteins, and the design of novel elastomeric proteins that exhibit stimuli-responsive mechanical properties. Moving forward, we are now exploring the use of these artificial elastomeric proteins as building blocks of protein-based biomaterials. Ultimately, we would like to rationally tailor mechanical properties of elastomeric protein-based materials by programming the molecular sequence, and thus nanomechanical properties, of elastomeric proteins at the single-molecule level. This step would help bridge the gap between single protein mechanics and material biomechanics, revealing how the mechanical properties of individual elastomeric proteins are translated into the properties of macroscopic materials.

  17. National Computer Security Conference Proceedings (12th): Information Systems Security: Solutions for Today - Concepts for Tomorrow Held in Baltimore, Maryland on 10-13 October 1989

    DTIC Science & Technology

    1989-10-13

    and other non -technical aspects of the system). System-wide Perspective. The systerm that is being designed and engineered must include not just the...specifications and is regarded as the lowest-level (implementation) of detail.-’ Ihis decomposition follows the typical "top down" design methodology ...formal verification process has contributed to the security and correctness of the TCB design and implementation. FORMUL METHODOLOGY DESCRIPTION The

  18. Leadership, Management, and Organization for National Security Space: Report to Congress of the Independent Assessment Panel on the Organization and Management of National Security Space

    DTIC Science & Technology

    2008-07-01

    Engineering. General Ronald R. Fogleman, United States Air Force - Retired General Ron Fogleman is the Chairman of the Board of The Durango ...serves on the board of directors for AAR Corp, Alpha Security, Alliant Techsystems, First National Bank of Durango , Integrated Data Corporation...headquartered in McLean, Virginia. He is based in Colorado Springs. Specializing in strategic planning and the management of complex organizations

  19. Rationally designed synthetic protein hydrogels with predictable mechanical properties.

    PubMed

    Wu, Junhua; Li, Pengfei; Dong, Chenling; Jiang, Heting; Bin Xue; Gao, Xiang; Qin, Meng; Wang, Wei; Bin Chen; Cao, Yi

    2018-02-12

    Designing synthetic protein hydrogels with tailored mechanical properties similar to naturally occurring tissues is an eternal pursuit in tissue engineering and stem cell and cancer research. However, it remains challenging to correlate the mechanical properties of protein hydrogels with the nanomechanics of individual building blocks. Here we use single-molecule force spectroscopy, protein engineering and theoretical modeling to prove that the mechanical properties of protein hydrogels are predictable based on the mechanical hierarchy of the cross-linkers and the load-bearing modules at the molecular level. These findings provide a framework for rationally designing protein hydrogels with independently tunable elasticity, extensibility, toughness and self-healing. Using this principle, we demonstrate the engineering of self-healable muscle-mimicking hydrogels that can significantly dissipate energy through protein unfolding. We expect that this principle can be generalized for the construction of protein hydrogels with customized mechanical properties for biomedical applications.

  20. Pharmacokinetic and pharmacodynamic considerations for the next generation protein therapeutics.

    PubMed

    Shah, Dhaval K

    2015-10-01

    Increasingly sophisticated protein engineering efforts have been undertaken lately to generate protein therapeutics with desired properties. This has resulted in the discovery of the next generation of protein therapeutics, which include: engineered antibodies, immunoconjugates, bi/multi-specific proteins, antibody mimetic novel scaffolds, and engineered ligands/receptors. These novel protein therapeutics possess unique physicochemical properties and act via a unique mechanism-of-action, which collectively makes their pharmacokinetics (PK) and pharmacodynamics (PD) different than other established biological molecules. Consequently, in order to support the discovery and development of these next generation molecules, it becomes important to understand the determinants controlling their PK/PD. This review discusses the determinants that a PK/PD scientist should consider during the design and development of next generation protein therapeutics. In addition, the role of systems PK/PD models in enabling rational development of the next generation protein therapeutics is emphasized.

  1. Pharmacokinetic and pharmacodynamic considerations for the next generation protein therapeutics

    PubMed Central

    Shah, Dhaval K.

    2015-01-01

    Increasingly sophisticated protein engineering efforts have been undertaken lately to generate protein therapeutics with desired properties. This has resulted in the discovery of the next generation of protein therapeutics, which include: engineered antibodies, immunoconjugates, bi/multi-specific proteins, antibody mimetic novel scaffolds, and engineered ligands/receptors. These novel protein therapeutics possess unique physicochemical properties and act via a unique mechanism-of-action, which collectively makes their pharmacokinetics (PK) and pharmacodynamics (PD) different than other established biological molecules. Consequently, in order to support the discovery and development of these next generation molecules, it becomes important to understand the determinants controlling their PK/PD. This review discusses the determinants that a PK/PD scientist should consider during the design and development of next generation protein therapeutics. In addition, the role of systems PK/PD models in enabling rational development of the next generation protein therapeutics is emphasized. PMID:26373957

  2. Glycopolymer functionalization of engineered spider silk protein-based materials for improved cell adhesion.

    PubMed

    Hardy, John G; Pfaff, André; Leal-Egaña, Aldo; Müller, Axel H E; Scheibel, Thomas R

    2014-07-01

    Silk protein-based materials are promising biomaterials for application as tissue scaffolds, due to their processability, biocompatibility, and biodegradability. The preparation of films composed of an engineered spider silk protein (eADF4(C16)) and their functionalization with glycopolymers are described. The glycopolymers bind proteins found in the extracellular matrix, providing a biomimetic coating on the films that improves cell adhesion to the surfaces of engineered spider silk films. Such silk-based materials have potential as coatings for degradable implantable devices. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Iris Cryptography for Security Purpose

    NASA Astrophysics Data System (ADS)

    Ajith, Srighakollapu; Balaji Ganesh Kumar, M.; Latha, S.; Samiappan, Dhanalakshmi; Muthu, P.

    2018-04-01

    In today's world, the security became the major issue to every human being. A major issue is hacking as hackers are everywhere, as the technology was developed still there are many issues where the technology fails to meet the security. Engineers, scientists were discovering the new products for security purpose as biometrics sensors like face recognition, pattern recognition, gesture recognition, voice authentication etcetera. But these devices fail to reach the expected results. In this work, we are going to present an approach to generate a unique secure key using the iris template. Here the iris templates are processed using the well-defined processing techniques. Using the encryption and decryption process they are stored, traversed and utilized. As of the work, we can conclude that the iris cryptography gives us the expected results for securing the data from eavesdroppers.

  4. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Moore, Thomas W.; Quach, Tu-Thach; Detry, Richard Joseph

    Complex Adaptive Systems of Systems, or CASoS, are vastly complex ecological, sociological, economic and/or technical systems which we must understand to design a secure future for the nation and the world. Perturbations/disruptions in CASoS have the potential for far-reaching effects due to pervasive interdependencies and attendant vulnerabilities to cascades in associated systems. Phoenix was initiated to address this high-impact problem space as engineers. Our overarching goals are maximizing security, maximizing health, and minimizing risk. We design interventions, or problem solutions, that influence CASoS to achieve specific aspirations. Through application to real-world problems, Phoenix is evolving the principles and discipline ofmore » CASoS Engineering while growing a community of practice and the CASoS engineers to populate it. Both grounded in reality and working to extend our understanding and control of that reality, Phoenix is at the same time a solution within a CASoS and a CASoS itself.« less

  5. Protein design and engineering of a de novo pathway for microbial production of 1,3-propanediol from glucose.

    PubMed

    Chen, Zhen; Geng, Feng; Zeng, An-Ping

    2015-02-01

    Protein engineering to expand the substrate spectrum of native enzymes opens new possibilities for bioproduction of valuable chemicals from non-natural pathways. No natural microorganism can directly use sugars to produce 1,3-propanediol (PDO). Here, we present a de novo route for the biosynthesis of PDO from sugar, which may overcome the mentioned limitations by expanding the homoserine synthesis pathway. The accomplishment of pathway from homoserine to PDO is achieved by protein engineering of glutamate dehydrogenase (GDH) and pyruvate decarboxylase to sequentially convert homoserine to 4-hydroxy-2-ketobutyrate and 3-hydroxypropionaldehyde. The latter is finally converted to PDO by using a native alcohol dehydrogenase. In this work, we report on experimental accomplishment of this non-natural pathway, especially by protein engineering of GDH for the key step of converting homoserine to 4-hydroxy-2-ketobutyrate. These results show the feasibility and significance of protein engineering for de novo pathway design and overproduction of desired industrial products. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  6. Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals

    PubMed Central

    Thess, Andreas; Grund, Stefanie; Mui, Barbara L; Hope, Michael J; Baumhof, Patrick; Fotin-Mleczek, Mariola; Schlake, Thomas

    2015-01-01

    Being a transient carrier of genetic information, mRNA could be a versatile, flexible, and safe means for protein therapies. While recent findings highlight the enormous therapeutic potential of mRNA, evidence that mRNA-based protein therapies are feasible beyond small animals such as mice is still lacking. Previous studies imply that mRNA therapeutics require chemical nucleoside modifications to obtain sufficient protein expression and avoid activation of the innate immune system. Here we show that chemically unmodified mRNA can achieve those goals as well by applying sequence-engineered molecules. Using erythropoietin (EPO) driven production of red blood cells as the biological model, engineered Epo mRNA elicited meaningful physiological responses from mice to nonhuman primates. Even in pigs of about 20 kg in weight, a single adequate dose of engineered mRNA encapsulated in lipid nanoparticles (LNPs) induced high systemic Epo levels and strong physiological effects. Our results demonstrate that sequence-engineered mRNA has the potential to revolutionize human protein therapies. PMID:26050989

  7. 16-Lead ECG Changes with Coronary Angioplasty - Location of ST-T Changes with Balloon Occlusion of Five Arterial Perfusion Beds

    DTIC Science & Technology

    1991-08-01

    Southeastern Center for Electrical Engineering REPORT NUMBER Education (SCEEE) 11th and Massachusetts Avenues St Cloud , FL 34769 D T |C 9. SPONSORING...performing the report. Self-explanatory. Enter U.S. Security Classification in accordance with U.S. Security Block 9. S Qonsorin/ Monitorig Aaencv

  8. A&M. Radioactive parts security storage area, heat removal storage casks. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    A&M. Radioactive parts security storage area, heat removal storage casks. Plan, section, and details. Ralph M. Parsons 1480-7 ANP/GE-3-720-S-1. Date: November 1958. Approved by INEEL Classification Office for public release. INEEL index no. 034-0720-60-693-107459 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

  9. Information Operations & Security

    DTIC Science & Technology

    2012-03-05

    Fred B. Schneider, Cornell The Promise of Security Metrics • Users: Purchasing decisions – Which system is the better value? • Builders ...Engineering University of Maryland, College Park DISTRIBUTION A: Approved for public release; distribution is unlimited. Digital Multimedia Anti...fingerprints for multimedia content: • Determine the time and place of recordings • Detect tampering in the multimedia content; bind video and

  10. Improved Wireless Security through Physical Layer Protocol Manipulation and Radio Frequency Fingerprinting

    DTIC Science & Technology

    2014-09-18

    radios in a cognitive radio network using a radio frequency fingerprinting based method. In IEEE International Conference on Communications (ICC...IMPROVEDWIRELESS SECURITY THROUGH PHYSICAL LAYER PROTOCOL MANIPULATION AND RADIO FREQUENCY FINGERPRINTING DISSERTATION Benjamin W. Ramsey, Captain...PHYSICAL LAYER PROTOCOL MANIPULATION AND RADIO FREQUENCY FINGERPRINTING DISSERTATION Presented to the Faculty Graduate School of Engineering and

  11. Skills Conversion Project: Chapter 9, Security Systems and Criminal Justice. Final Report.

    ERIC Educational Resources Information Center

    National Society of Professional Engineers, Washington, DC.

    This report provides the findings of two skills conversion research teams on the placement of displaced aerospace and defense engineers, scientists, and technicians in the fields of security systems and criminal justice. The teams, located in Philadelphia and San Jose, concluded independently that a minimum of 2,000 positions could be created in…

  12. CMMI(Registered) for Acquisition, Version 1.3. CMMI-ACQ, V1.3

    DTIC Science & Technology

    2010-11-01

    and Software Engineering – System Life Cycle Processes [ ISO 2008b] ISO /IEC 27001 :2005 Information technology – Security techniques – Information...International Organization for Standardization and International Electrotechnical Commission. ISO /IEC 27001 Information Technology – Security Techniques...International Organization for Standardization/International Electrotechnical Commission ( ISO /IEC) body of standards. CMMs focus on improving processes

  13. CORBASec Used to Secure Distributed Aerospace Propulsion Simulations

    NASA Technical Reports Server (NTRS)

    Blaser, Tammy M.

    2003-01-01

    The NASA Glenn Research Center and its industry partners are developing a Common Object Request Broker (CORBA) Security (CORBASec) test bed to secure their distributed aerospace propulsion simulations. Glenn has been working with its aerospace propulsion industry partners to deploy the Numerical Propulsion System Simulation (NPSS) object-based technology. NPSS is a program focused on reducing the cost and time in developing aerospace propulsion engines. It was developed by Glenn and is being managed by the NASA Ames Research Center as the lead center reporting directly to NASA Headquarters' Aerospace Technology Enterprise. Glenn is an active domain member of the Object Management Group: an open membership, not-for-profit consortium that produces and manages computer industry specifications (i.e., CORBA) for interoperable enterprise applications. When NPSS is deployed, it will assemble a distributed aerospace propulsion simulation scenario from proprietary analytical CORBA servers and execute them with security afforded by the CORBASec implementation. The NPSS CORBASec test bed was initially developed with the TPBroker Security Service product (Hitachi Computer Products (America), Inc., Waltham, MA) using the Object Request Broker (ORB), which is based on the TPBroker Basic Object Adaptor, and using NPSS software across different firewall products. The test bed has been migrated to the Portable Object Adaptor architecture using the Hitachi Security Service product based on the VisiBroker 4.x ORB (Borland, Scotts Valley, CA) and on the Orbix 2000 ORB (Dublin, Ireland, with U.S. headquarters in Waltham, MA). Glenn, GE Aircraft Engines, and Pratt & Whitney Aircraft are the initial industry partners contributing to the NPSS CORBASec test bed. The test bed uses Security SecurID (RSA Security Inc., Bedford, MA) two-factor token-based authentication together with Hitachi Security Service digital-certificate-based authentication to validate the various NPSS users. The test bed is expected to demonstrate NPSS CORBASec-specific policy functionality, confirm adequate performance, and validate the required Internet configuration in a distributed collaborative aerospace propulsion environment.

  14. FY10 Engineering Innovations, Research and Technology Report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lane, M A; Aceves, S M; Paulson, C N

    This report summarizes key research, development, and technology advancements in Lawrence Livermore National Laboratory's Engineering Directorate for FY2010. These efforts exemplify Engineering's nearly 60-year history of developing and applying the technology innovations needed for the Laboratory's national security missions, and embody Engineering's mission to ''Enable program success today and ensure the Laboratory's vitality tomorrow.'' Leading off the report is a section featuring compelling engineering innovations. These innovations range from advanced hydrogen storage that enables clean vehicles, to new nuclear material detection technologies, to a landmine detection system using ultra-wideband ground-penetrating radar. Many have been recognized with R&D Magazine's prestigious R&Dmore » 100 Award; all are examples of the forward-looking application of innovative engineering to pressing national problems and challenging customer requirements. Engineering's capability development strategy includes both fundamental research and technology development. Engineering research creates the competencies of the future where discovery-class groundwork is required. Our technology development (or reduction to practice) efforts enable many of the research breakthroughs across the Laboratory to translate from the world of basic research to the national security missions of the Laboratory. This portfolio approach produces new and advanced technological capabilities, and is a unique component of the value proposition of the Lawrence Livermore Laboratory. The balance of the report highlights this work in research and technology, organized into thematic technical areas: Computational Engineering; Micro/Nano-Devices and Structures; Measurement Technologies; Engineering Systems for Knowledge Discovery; and Energy Manipulation. Our investments in these areas serve not only known programmatic requirements of today and tomorrow, but also anticipate the breakthrough engineering innovations that will be needed in the future.« less

  15. In-depth analysis of protein inference algorithms using multiple search engines and well-defined metrics.

    PubMed

    Audain, Enrique; Uszkoreit, Julian; Sachsenberg, Timo; Pfeuffer, Julianus; Liang, Xiao; Hermjakob, Henning; Sanchez, Aniel; Eisenacher, Martin; Reinert, Knut; Tabb, David L; Kohlbacher, Oliver; Perez-Riverol, Yasset

    2017-01-06

    In mass spectrometry-based shotgun proteomics, protein identifications are usually the desired result. However, most of the analytical methods are based on the identification of reliable peptides and not the direct identification of intact proteins. Thus, assembling peptides identified from tandem mass spectra into a list of proteins, referred to as protein inference, is a critical step in proteomics research. Currently, different protein inference algorithms and tools are available for the proteomics community. Here, we evaluated five software tools for protein inference (PIA, ProteinProphet, Fido, ProteinLP, MSBayesPro) using three popular database search engines: Mascot, X!Tandem, and MS-GF+. All the algorithms were evaluated using a highly customizable KNIME workflow using four different public datasets with varying complexities (different sample preparation, species and analytical instruments). We defined a set of quality control metrics to evaluate the performance of each combination of search engines, protein inference algorithm, and parameters on each dataset. We show that the results for complex samples vary not only regarding the actual numbers of reported protein groups but also concerning the actual composition of groups. Furthermore, the robustness of reported proteins when using databases of differing complexities is strongly dependant on the applied inference algorithm. Finally, merging the identifications of multiple search engines does not necessarily increase the number of reported proteins, but does increase the number of peptides per protein and thus can generally be recommended. Protein inference is one of the major challenges in MS-based proteomics nowadays. Currently, there are a vast number of protein inference algorithms and implementations available for the proteomics community. Protein assembly impacts in the final results of the research, the quantitation values and the final claims in the research manuscript. Even though protein inference is a crucial step in proteomics data analysis, a comprehensive evaluation of the many different inference methods has never been performed. Previously Journal of proteomics has published multiple studies about other benchmark of bioinformatics algorithms (PMID: 26585461; PMID: 22728601) in proteomics studies making clear the importance of those studies for the proteomics community and the journal audience. This manuscript presents a new bioinformatics solution based on the KNIME/OpenMS platform that aims at providing a fair comparison of protein inference algorithms (https://github.com/KNIME-OMICS). Six different algorithms - ProteinProphet, MSBayesPro, ProteinLP, Fido and PIA- were evaluated using the highly customizable workflow on four public datasets with varying complexities. Five popular database search engines Mascot, X!Tandem, MS-GF+ and combinations thereof were evaluated for every protein inference tool. In total >186 proteins lists were analyzed and carefully compare using three metrics for quality assessments of the protein inference results: 1) the numbers of reported proteins, 2) peptides per protein, and the 3) number of uniquely reported proteins per inference method, to address the quality of each inference method. We also examined how many proteins were reported by choosing each combination of search engines, protein inference algorithms and parameters on each dataset. The results show that using 1) PIA or Fido seems to be a good choice when studying the results of the analyzed workflow, regarding not only the reported proteins and the high-quality identifications, but also the required runtime. 2) Merging the identifications of multiple search engines gives almost always more confident results and increases the number of peptides per protein group. 3) The usage of databases containing not only the canonical, but also known isoforms of proteins has a small impact on the number of reported proteins. The detection of specific isoforms could, concerning the question behind the study, compensate for slightly shorter reports using the parsimonious reports. 4) The current workflow can be easily extended to support new algorithms and search engine combinations. Copyright © 2016. Published by Elsevier B.V.

  16. Algorithms for database-dependent search of MS/MS data.

    PubMed

    Matthiesen, Rune

    2013-01-01

    The frequent used bottom-up strategy for identification of proteins and their associated modifications generate nowadays typically thousands of MS/MS spectra that normally are matched automatically against a protein sequence database. Search engines that take as input MS/MS spectra and a protein sequence database are referred as database-dependent search engines. Many programs both commercial and freely available exist for database-dependent search of MS/MS spectra and most of the programs have excellent user documentation. The aim here is therefore to outline the algorithm strategy behind different search engines rather than providing software user manuals. The process of database-dependent search can be divided into search strategy, peptide scoring, protein scoring, and finally protein inference. Most efforts in the literature have been put in to comparing results from different software rather than discussing the underlining algorithms. Such practical comparisons can be cluttered by suboptimal implementation and the observed differences are frequently caused by software parameters settings which have not been set proper to allow even comparison. In other words an algorithmic idea can still be worth considering even if the software implementation has been demonstrated to be suboptimal. The aim in this chapter is therefore to split the algorithms for database-dependent searching of MS/MS data into the above steps so that the different algorithmic ideas become more transparent and comparable. Most search engines provide good implementations of the first three data analysis steps mentioned above, whereas the final step of protein inference are much less developed for most search engines and is in many cases performed by an external software. The final part of this chapter illustrates how protein inference is built into the VEMS search engine and discusses a stand-alone program SIR for protein inference that can import a Mascot search result.

  17. Tissue engineering skeletal muscle for orthopaedic applications

    NASA Technical Reports Server (NTRS)

    Payumo, Francis C.; Kim, Hyun D.; Sherling, Michael A.; Smith, Lee P.; Powell, Courtney; Wang, Xiao; Keeping, Hugh S.; Valentini, Robert F.; Vandenburgh, Herman H.

    2002-01-01

    With current technology, tissue-engineered skeletal muscle analogues (bioartificial muscles) generate too little active force to be clinically useful in orthopaedic applications. They have been engineered genetically with numerous transgenes (growth hormone, insulinlike growth factor-1, erythropoietin, vascular endothelial growth factor), and have been shown to deliver these therapeutic proteins either locally or systemically for months in vivo. Bone morphogenetic proteins belonging to the transforming growth factor-beta superfamily are osteoinductive molecules that drive the differentiation pathway of mesenchymal cells toward the chondroblastic or osteoblastic lineage, and stimulate bone formation in vivo. To determine whether skeletal muscle cells endogenously expressing bone morphogenetic proteins might serve as a vehicle for systemic bone morphogenetic protein delivery in vivo, proliferating skeletal myoblasts (C2C12) were transduced with a replication defective retrovirus containing the gene for recombinant human bone morphogenetic protein-6 (C2BMP-6). The C2BMP-6 cells constitutively expressed recombinant human bone morphogenetic protein-6 and synthesized bioactive recombinant human bone morphogenetic protein-6, based on increased alkaline phosphatase activity in coincubated mesenchymal cells. C2BMP-6 cells did not secrete soluble, bioactive recombinant human bone morphogenetic protein-6, but retained the bioactivity in the cell layer. Therefore, genetically-engineered skeletal muscle cells might serve as a platform for long-term delivery of osteoinductive bone morphogenetic proteins locally.

  18. Clinical translation of controlled protein delivery systems for tissue engineering.

    PubMed

    Spiller, Kara L; Vunjak-Novakovic, Gordana

    2015-04-01

    Strategies that utilize controlled release of drugs and proteins for tissue engineering have enormous potential to regenerate damaged organs and tissues. The multiple advantages of controlled release strategies merit overcoming the significant challenges to translation, including high costs and long, difficult regulatory pathways. This review highlights the potential of controlled release of proteins for tissue engineering and regenerative medicine. We specifically discuss treatment modalities that have reached preclinical and clinical trials, with emphasis on controlled release systems for bone tissue engineering, the most advanced application with several products already in clinic. Possible strategies to address translational and regulatory concerns are also discussed.

  19. Clinical translation of controlled protein delivery systems for tissue engineering

    PubMed Central

    Spiller, Kara L.; Vunjak-Novakovic, Gordana

    2013-01-01

    Strategies that utilize controlled release of drugs and proteins for tissue engineering have enormous potential to regenerate damaged organs and tissues. The multiple advantages of controlled release strategies merit overcoming the significant challenges to translation, including high costs and long, difficult regulatory pathways. This review highlights the potential of controlled release of proteins for tissue engineering and regenerative medicine. We specifically discuss treatment modalities that have reached preclinical and clinical trials, with emphasis on controlled release systems for bone tissue engineering, the most advanced application with several products already in clinic. Possible strategies to address translational and regulatory concerns are also discussed. PMID:25787736

  20. Modular protein domains: an engineering approach toward functional biomaterials.

    PubMed

    Lin, Charng-Yu; Liu, Julie C

    2016-08-01

    Protein domains and peptide sequences are a powerful tool for conferring specific functions to engineered biomaterials. Protein sequences with a wide variety of functionalities, including structure, bioactivity, protein-protein interactions, and stimuli responsiveness, have been identified, and advances in molecular biology continue to pinpoint new sequences. Protein domains can be combined to make recombinant proteins with multiple functionalities. The high fidelity of the protein translation machinery results in exquisite control over the sequence of recombinant proteins and the resulting properties of protein-based materials. In this review, we discuss protein domains and peptide sequences in the context of functional protein-based materials, composite materials, and their biological applications. Copyright © 2016 Elsevier Ltd. All rights reserved.

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